LOCUS       DAZUCE010000001        80328 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZUCE010000001 DAZUCE010000000
VERSION     DAZUCE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.001"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            188..532
                     /gene="yejG"
                     /locus_tag="ACHY03_000001"
     CDS             188..532
                     /gene="yejG"
                     /locus_tag="ACHY03_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFS3903295.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(536..2125)
                     /gene="yejF"
                     /locus_tag="ACHY03_000002"
     CDS             complement(536..2125)
                     /gene="yejF"
                     /locus_tag="ACHY03_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFS3903296.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(2127..3152)
                     /gene="yejE"
                     /locus_tag="ACHY03_000003"
     CDS             complement(2127..3152)
                     /gene="yejE"
                     /locus_tag="ACHY03_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3903297.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(3152..4203)
                     /gene="yejB"
                     /locus_tag="ACHY03_000004"
                     /pseudo
     CDS             complement(3152..4203)
                     /gene="yejB"
                     /locus_tag="ACHY03_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
     gene            complement(4204..6018)
                     /gene="yejA"
                     /locus_tag="ACHY03_000005"
     CDS             complement(4204..6018)
                     /gene="yejA"
                     /locus_tag="ACHY03_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFS3903298.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSRIIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(6100..7656)
                     /gene="pdeN"
                     /locus_tag="ACHY03_000006"
     CDS             complement(6100..7656)
                     /gene="pdeN"
                     /locus_tag="ACHY03_000006"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFS3903299.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITEHD
                     MLKEQEATQLFAWLHSVGIEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKSLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(7837..8403)
                     /gene="mepS"
                     /locus_tag="ACHY03_000007"
     CDS             complement(7837..8403)
                     /gene="mepS"
                     /locus_tag="ACHY03_000007"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFS3903300.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(8815..9528)
                     /gene="lpxT"
                     /locus_tag="ACHY03_000008"
     CDS             complement(8815..9528)
                     /gene="lpxT"
                     /locus_tag="ACHY03_000008"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFS3903301.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSSF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(9567..10553)
                     /gene="yeiR"
                     /locus_tag="ACHY03_000009"
     CDS             complement(9567..10553)
                     /gene="yeiR"
                     /locus_tag="ACHY03_000009"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFS3903302.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(10671..12137)
                     /gene="yeiQ"
                     /locus_tag="ACHY03_000010"
     CDS             complement(10671..12137)
                     /gene="yeiQ"
                     /locus_tag="ACHY03_000010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS3903303.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIKQAWQQIAQFGAHQALLNTLKI"
     gene            complement(12360..12932)
                     /gene="yeiP"
                     /locus_tag="ACHY03_000011"
     CDS             complement(12360..12932)
                     /gene="yeiP"
                     /locus_tag="ACHY03_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFS3903304.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            13087..13341
                     /gene="yeiW"
                     /locus_tag="ACHY03_000012"
     CDS             13087..13341
                     /gene="yeiW"
                     /locus_tag="ACHY03_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFS3903305.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKV
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(13338..14519)
                     /gene="setB"
                     /locus_tag="ACHY03_000013"
     CDS             complement(13338..14519)
                     /gene="setB"
                     /locus_tag="ACHY03_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFS3903306.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            14887..16017
                     /gene="fruB"
                     /locus_tag="ACHY03_000014"
     CDS             14887..16017
                     /gene="fruB"
                     /locus_tag="ACHY03_000014"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFS3903307.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            16017..16955
                     /gene="fruK"
                     /locus_tag="ACHY03_000015"
     CDS             16017..16955
                     /gene="fruK"
                     /locus_tag="ACHY03_000015"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFS3903308.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            16972..18663
                     /gene="fruA"
                     /locus_tag="ACHY03_000016"
     CDS             16972..18663
                     /gene="fruA"
                     /locus_tag="ACHY03_000016"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFS3903309.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            19087..20028
                     /gene="psuK"
                     /locus_tag="ACHY03_000017"
     CDS             19087..20028
                     /gene="psuK"
                     /locus_tag="ACHY03_000017"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFS3903310.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            20016..20954
                     /gene="psuG"
                     /locus_tag="ACHY03_000018"
     CDS             20016..20954
                     /gene="psuG"
                     /locus_tag="ACHY03_000018"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFS3903311.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            21048..22298
                     /locus_tag="ACHY03_000019"
     CDS             21048..22298
                     /locus_tag="ACHY03_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFS3903312.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSEMPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(22366..23064)
                     /gene="yeiL"
                     /locus_tag="ACHY03_000020"
     CDS             complement(22366..23064)
                     /gene="yeiL"
                     /locus_tag="ACHY03_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFS3903313.1"
                     /translation="MKEIHNNDLKLQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            23194..24135
                     /gene="rihB"
                     /locus_tag="ACHY03_000021"
     CDS             23194..24135
                     /gene="rihB"
                     /locus_tag="ACHY03_000021"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFS3903314.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            24235..25485
                     /gene="nupX"
                     /locus_tag="ACHY03_000022"
     CDS             24235..25485
                     /gene="nupX"
                     /locus_tag="ACHY03_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFS3903315.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(25541..26629)
                     /gene="yeiI"
                     /locus_tag="ACHY03_000023"
     CDS             complement(25541..26629)
                     /gene="yeiI"
                     /locus_tag="ACHY03_000023"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFS3903316.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLMPMV"
     gene            complement(26632..27489)
                     /gene="nfo"
                     /locus_tag="ACHY03_000024"
     CDS             complement(26632..27489)
                     /gene="nfo"
                     /locus_tag="ACHY03_000024"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFS3903317.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHTFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(27563..28612)
                     /gene="yeiH"
                     /locus_tag="ACHY03_000025"
     CDS             complement(27563..28612)
                     /gene="yeiH"
                     /locus_tag="ACHY03_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFS3903318.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            28711..29592
                     /gene="yieE"
                     /locus_tag="ACHY03_000026"
     CDS             28711..29592
                     /gene="yieE"
                     /locus_tag="ACHY03_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFS3903319.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPC"
     gene            29797..31266
                     /gene="lysP"
                     /locus_tag="ACHY03_000027"
     CDS             29797..31266
                     /gene="lysP"
                     /locus_tag="ACHY03_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFS3903320.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPGKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            31558..33537
                     /gene="cirA"
                     /locus_tag="ACHY03_000028"
     CDS             31558..33537
                     /gene="cirA"
                     /locus_tag="ACHY03_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFS3903321.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33569..34405)
                     /gene="yeiG"
                     /locus_tag="ACHY03_000029"
     CDS             complement(33569..34405)
                     /gene="yeiG"
                     /locus_tag="ACHY03_000029"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFS3903322.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            34663..35331
                     /gene="folE"
                     /locus_tag="ACHY03_000030"
     CDS             34663..35331
                     /gene="folE"
                     /locus_tag="ACHY03_000030"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFS3903323.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            35348..36505
                     /gene="yeiB"
                     /locus_tag="ACHY03_000031"
     CDS             35348..36505
                     /gene="yeiB"
                     /locus_tag="ACHY03_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFS3903324.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            36647..37687
                     /gene="galS"
                     /locus_tag="ACHY03_000032"
     CDS             36647..37687
                     /gene="galS"
                     /locus_tag="ACHY03_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFS3903325.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            37967..38965
                     /gene="mglB"
                     /locus_tag="ACHY03_000033"
     CDS             37967..38965
                     /gene="mglB"
                     /locus_tag="ACHY03_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFS3903326.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            39026..40546
                     /gene="mglA"
                     /locus_tag="ACHY03_000034"
     CDS             39026..40546
                     /gene="mglA"
                     /locus_tag="ACHY03_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFS3903327.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            40562..41572
                     /gene="mglC"
                     /locus_tag="ACHY03_000035"
     CDS             40562..41572
                     /gene="mglC"
                     /locus_tag="ACHY03_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFS3903328.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(41815..43050)
                     /gene="preA"
                     /locus_tag="ACHY03_000036"
     CDS             complement(41815..43050)
                     /gene="preA"
                     /locus_tag="ACHY03_000036"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFS3903329.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPILAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(43044..44282)
                     /gene="preT"
                     /locus_tag="ACHY03_000037"
     CDS             complement(43044..44282)
                     /gene="preT"
                     /locus_tag="ACHY03_000037"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFS3903330.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQCARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MVADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(44476..44715)
                     /gene="yeiS"
                     /locus_tag="ACHY03_000038"
     CDS             complement(44476..44715)
                     /gene="yeiS"
                     /locus_tag="ACHY03_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFS3903331.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(44718..45437)
                     /gene="sanA"
                     /locus_tag="ACHY03_000039"
     CDS             complement(44718..45437)
                     /gene="sanA"
                     /locus_tag="ACHY03_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFS3903332.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(45587..46471)
                     /gene="cdd"
                     /locus_tag="ACHY03_000040"
     CDS             complement(45587..46471)
                     /gene="cdd"
                     /locus_tag="ACHY03_000040"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFS3903333.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(46601..47296)
                     /gene="yohK"
                     /locus_tag="ACHY03_000041"
     CDS             complement(46601..47296)
                     /gene="yohK"
                     /locus_tag="ACHY03_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFS3903334.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(47293..47691)
                     /gene="yohJ"
                     /locus_tag="ACHY03_000042"
     CDS             complement(47293..47691)
                     /gene="yohJ"
                     /locus_tag="ACHY03_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFS3903335.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            complement(47822..48730)
                     /locus_tag="ACHY03_000043"
     CDS             complement(47822..48730)
                     /locus_tag="ACHY03_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3903336.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            48857..50215
                     /locus_tag="ACHY03_000044"
     CDS             48857..50215
                     /locus_tag="ACHY03_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3903337.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            50227..51255
                     /gene="gtdA"
                     /locus_tag="ACHY03_000045"
     CDS             50227..51255
                     /gene="gtdA"
                     /locus_tag="ACHY03_000045"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HFS3903338.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            51271..51972
                     /locus_tag="ACHY03_000046"
     CDS             51271..51972
                     /locus_tag="ACHY03_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFS3903339.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            51981..52625
                     /gene="maiA"
                     /locus_tag="ACHY03_000047"
     CDS             51981..52625
                     /gene="maiA"
                     /locus_tag="ACHY03_000047"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HFS3903340.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            52640..53833
                     /locus_tag="ACHY03_000048"
     CDS             52640..53833
                     /locus_tag="ACHY03_000048"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HFS3903341.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            53985..54935
                     /gene="dusC"
                     /locus_tag="ACHY03_000049"
     CDS             53985..54935
                     /gene="dusC"
                     /locus_tag="ACHY03_000049"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFS3903342.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSHDIARAIQAIDIDKLR"
     gene            complement(55323..55406)
                     /gene="yohP"
                     /locus_tag="ACHY03_000050"
     CDS             complement(55323..55406)
                     /gene="yohP"
                     /locus_tag="ACHY03_000050"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFS3903343.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            55630..57066
                     /gene="mdtQ"
                     /locus_tag="ACHY03_000051"
     CDS             55630..57066
                     /gene="mdtQ"
                     /locus_tag="ACHY03_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFS3903344.1"
                     /translation="MNRDSFYPAIACFPLLLMLTGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            57119..57880
                     /gene="yohF"
                     /locus_tag="ACHY03_000052"
     CDS             57119..57880
                     /gene="yohF"
                     /locus_tag="ACHY03_000052"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3903345.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(58010..58588)
                     /gene="yohD"
                     /locus_tag="ACHY03_000053"
     CDS             complement(58010..58588)
                     /gene="yohD"
                     /locus_tag="ACHY03_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFS3903346.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            58758..59345
                     /gene="yohC"
                     /locus_tag="ACHY03_000054"
     CDS             58758..59345
                     /gene="yohC"
                     /locus_tag="ACHY03_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFS3903347.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            59519..60451
                     /gene="pbpG"
                     /locus_tag="ACHY03_000055"
     CDS             59519..60451
                     /gene="pbpG"
                     /locus_tag="ACHY03_000055"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFS3903348.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(60490..62205)
                     /gene="dld"
                     /locus_tag="ACHY03_000056"
     CDS             complement(60490..62205)
                     /gene="dld"
                     /locus_tag="ACHY03_000056"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFS3903349.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            62401..64698
                     /gene="bglX"
                     /locus_tag="ACHY03_000057"
     CDS             62401..64698
                     /gene="bglX"
                     /locus_tag="ACHY03_000057"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFS3903350.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVDAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            64950..65867
                     /gene="osmF"
                     /locus_tag="ACHY03_000058"
     CDS             64950..65867
                     /gene="osmF"
                     /locus_tag="ACHY03_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFS3903351.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            65874..67031
                     /gene="yehY"
                     /locus_tag="ACHY03_000059"
     CDS             65874..67031
                     /gene="yehY"
                     /locus_tag="ACHY03_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFS3903352.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            67024..67950
                     /gene="yehX"
                     /locus_tag="ACHY03_000060"
     CDS             67024..67950
                     /gene="yehX"
                     /locus_tag="ACHY03_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFS3903353.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLVEA"
     gene            67955..68686
                     /gene="yehW"
                     /locus_tag="ACHY03_000061"
     CDS             67955..68686
                     /gene="yehW"
                     /locus_tag="ACHY03_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFS3903354.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(68667..68774)
                     /gene="yohO"
                     /locus_tag="ACHY03_000062"
     CDS             complement(68667..68774)
                     /gene="yohO"
                     /locus_tag="ACHY03_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFS3903355.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(68834..69565)
                     /gene="mlrA"
                     /locus_tag="ACHY03_000063"
     CDS             complement(68834..69565)
                     /gene="mlrA"
                     /locus_tag="ACHY03_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFS3903356.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            69787..71472
                     /gene="btsS"
                     /locus_tag="ACHY03_000064"
     CDS             69787..71472
                     /gene="btsS"
                     /locus_tag="ACHY03_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFS3903357.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            71469..72188
                     /gene="btsR"
                     /locus_tag="ACHY03_000065"
                     /gene_synonym="yehT"
     CDS             71469..72188
                     /gene="btsR"
                     /locus_tag="ACHY03_000065"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFS3903358.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            72235..72705
                     /gene="yehS"
                     /locus_tag="ACHY03_000066"
     CDS             72235..72705
                     /gene="yehS"
                     /locus_tag="ACHY03_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFS3903359.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(72747..73208)
                     /gene="yehR"
                     /locus_tag="ACHY03_000067"
     CDS             complement(72747..73208)
                     /gene="yehR"
                     /locus_tag="ACHY03_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFS3903360.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YIYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKASGFKEVK"
     gene            complement(73333..75336)
                     /gene="yehQ"
                     /locus_tag="ACHY03_000068"
     CDS             complement(73333..75336)
                     /gene="yehQ"
                     /locus_tag="ACHY03_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFS3903361.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVTSLPSVSVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSVIGQAELLANDLTA
                     VGFYRLAHMLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(75333..76469)
                     /gene="yehP"
                     /locus_tag="ACHY03_000069"
     CDS             complement(75333..76469)
                     /gene="yehP"
                     /locus_tag="ACHY03_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFS3903362.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNSRQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVHQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(76462..78741)
                     /gene="yehM"
                     /locus_tag="ACHY03_000070"
     CDS             complement(76462..78741)
                     /gene="yehM"
                     /locus_tag="ACHY03_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFS3903363.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALARYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHASALAKMWRECPQEIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGKPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNAITEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DANFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(78752..79840)
                     /gene="yehL"
                     /locus_tag="ACHY03_000071"
     CDS             complement(78752..79840)
                     /gene="yehL"
                     /locus_tag="ACHY03_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFS3903364.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDTAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEGDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT        19399 a        20121 c        21401 g        19407 t
ORIGIN      
        1 atcgcagaaa cgggtacata tagccccgca aacgtgacca cgcccgcaga tattacttaa
       61 atcagagcca tagaggccac gcaggcgagg catcaatctt tacgatctgt ataaagacgg
      121 attgttgatg atgtgttaaa attgatgtaa ataaattgtg aagtaaatgt gcttccgggg
      181 aaaataagtg acttcattac aactctcaat agtccatcga ctgccgcaga actatcgctg
      241 gtctgctggt ttcgcaggtt cgaaggttga accgattccg caaaatggac cgtgcggtga
      301 caacagcctg gtggcgctta aattgcttag cccggatggt gataatgcat ggtcggtgat
      361 gtataaacta agccaggcgt taagcgacat cgaagttcca tgttcggtgc tggagtgtga
      421 aggtgagccg tgcctgtttg taaatcgcca ggacgagttt gctgcaacat gccgattgaa
      481 aaattttggt gtggcaattg ccgaaccctt ttcaaactac aatccttttt aagcgtcagc
      541 tcaacgccag tagctgacgc gtatactcct gctgcggtgt ggcaaacacg cgcgcgcacg
      601 gtccttgttc cactacttcc ccttgtcgca gtacgataac ctgatgacat aacgcgcgga
      661 caacgtgcaa atcgtggctg ataaacaaat aggccagttg atgcttttgt tgcaatgatt
      721 tcaatagcgt caatatttgc gcctgtaccg ttttgtcgag tgatgatgtc ggttcatcaa
      781 gtatgatcag cgagggctta agaattaatg ccctggcaat cgcaatacgt tgtcgctgac
      841 caccagagaa ctccgccgga taacggtggc gtgtttcagg atctaatccc acttcatgca
      901 tcacggctat cacttgttgt tcgcgttgtg cggcagaaag cgtcggctgg tgaacccgta
      961 agccttcctc aataatctgc aaaacgttga gtcgtgggtt gagcgaggag tttggatcct
     1021 gaaataccac ctgaatgcga tgacgaatag gtaacagctg gcggcgattt aaattttgca
     1081 gtggctgacc gtcaaagatg atgctgccct gagaattaat cagtcgcagc agcgccagtc
     1141 ccgtcgtgct tttcccggaa ccggactcgc ccactaaacc cagtgtttca cccgctcgta
     1201 gcgtaaaact gatgtttttc accaccacat tatgatccac aatgcgcttc aaaatccctt
     1261 tgcgaatggg gaaggcaacc tgaagctgtt caacatccag caacgttgag gcaggttctg
     1321 gcaatggcac cgggtcgcct gacggttcac tgttgagtag cttttgtgtg taaggatgag
     1381 tgggtgaggc aaatagcgta gcggcgttat tttgctcgac acagcgaccg ttttgcatta
     1441 ccgccacgcg gtgggccagt tttctgacaa tgctgaggtt atgagtaata aacagcatgc
     1501 ccatattcag ctcgccttgc agttcgcgca acagctgtaa aatctgcgcc tggacagaga
     1561 cgtccagtgc ggtggtcggt tcatcggcaa ttaataattc cggtcgcgtt aacagtgcca
     1621 tcgcaatcat cacccgctgg cgttcgccgc cggagagctg atgcggataa tctgtcagcc
     1681 gttttgccgc ctggcggata ccgacgcgat caaggcagtt aagaatttcg ccacgagccg
     1741 cttcccgacg catcccgcgg tggagtgaaa gcacttcata aagctgtttt tccagggtat
     1801 gcaatggatt taacgacacc atcggctcct gaaaaatcat ggcgatctta ttaccgcgta
     1861 caccgcgcaa cgtttgatcg ctggcatgaa gcagcgattc gccatgaaaa cgaatatcgc
     1921 cggagagata ttcaaccggc ggggatggga gcaggcgtaa aattgacagc gcggtaacgc
     1981 ttttgcctga acctgactca cccaccagcg ccagcgtttc gccagcctca atctgtagtg
     2041 aaacatcatt gactactgta cgtacggttt gctgatggcg aaaacccacc gacaaatttt
     2101 caatcgctaa cagagtttgc gtcatactac accgccttat taggatcaaa tgcgtcgcgg
     2161 acggcttcac caataaagat cagcaaagac aataatatcg ccaccgacaa gaaggcggtg
     2221 atcccaagcc acggagcctg aaggttattt ttcccttgta acagcagttc accgagtgac
     2281 ggtgaaccga gcggcagacc gaagccgagg aaatcgagtg aggtcagggt ggtgatcgaa
     2341 ctacataaaa taaacggtaa aaaggtgagg gtcgcgacca tggcattagg caacatatga
     2401 cgcaggatga tactgcgatc gctgacgcca agcgcctgcg ccgcacgaat gtagtcgaaa
     2461 ttacgggtac gtaaaaactc cgcccgcacc acgccgacca gactcatcca gccaaacaag
     2521 acagtaattg ccagcagcca ccagaagtta ggctgtacga cgctggaaag taaaataatc
     2581 aaaaacagcg tcggcatccc cgaccatact tcaataaagc gctgccccca gagatcgacc
     2641 ttaccgccgt aatagccttg tagcgccccc gccagcacgc ccatcacgct ggaacagaga
     2701 gtcagcatca ggccaaacag aaccgagatc cgcgtgccat agagaatacg tgccagcaca
     2761 tcgccgccgt tggcatccgt tcccagccag ttttgccggg aaggtgggga agggaagggc
     2821 ttatcggtag caaagttgat actggtagca ccaaagcgaa tcggtgccca cagtacccag
     2881 ccattatttt ccagccgttg tttcagccac gggtcctgat aatcagcctg acttgccagt
     2941 gggccgccaa aatcgctttc gctgtagttt ttcaacaacg ggaaatacca actgccgtca
     3001 taacgcacca gcaacggttt atcgttggcg ataagttcag aacacaaact caaaccaaac
     3061 aaaacgagga aaatccataa cgaccagtag ccgcgacggt tatggcgaaa acgcgcccaa
     3121 cgggcctgat tgacggggct gagtcgcgac attaacgtcc ctcaaaatct atacgcggat
     3181 cgaccagcgt atagctgata tcactgacaa tattcagcag caggccaatc agggtgaaaa
     3241 tatacaaggt accaaacatt acaggataat cgcgcgagac ggtcgcttcg tagcccagta
     3301 agccaagacc attgagtgaa aacatcacct caatcagcag cgagccggta aaaaacatgc
     3361 tgataaacgt cgccggaaaa ccggcaatca ccagcagcat ggcgttgcgg aacacatgtt
     3421 tccagagaat atttttttca cttacccctt tcgcacgcgc ggtcaccacg tattgcttgc
     3481 gcacttcatc aaggaatgag ttttttgtca gcatagtcag cgccgcaaag ccaccaatca
     3541 ccgtcgccag caccggcagc gtgatatgcc acagataatc ggtgattttc tgataccacg
     3601 gcagcgaatc aaagttagcg gaaaccaggc cgcgtagagg gaacaggtcg aaatagctgc
     3661 cgcctgcgaa gaagacaatc agcaggatgg caaacaaaaa ggccggaatg gcgtagccga
     3721 tgatgataaa tgcgctactc cagacgtcaa agcggctccc attataaaca gctttgcgaa
     3781 tgcctaacgg aatcgacacc agatagataa tcagcgtgct ccacaatccg agggtgatgg
     3841 aaaccggcag actgtcttta attcaaagcg gatgtagtcc cagagcattt tgaagtaacg
     3901 ttcgtggatc ggcttatcga aaccgtagcg atgagtgatc tcggcgatca cttctggatc
     3961 taatccgcgt ccgccacggt aattactgtc gctgatatta ccgacgcccg tttgcgcatg
     4021 gctggcgcga acgccttcac cgcctgcgcc gggtaatact ccggcattac caaactcaat
     4081 ggcggcgatg gcctggtcga ccggaccgcc aggcgcaatt tgcacgatga aaaagttgat
     4141 agtgataatc gcccataacg ttgggatcac cagcaacaga cggcgaatca gataagcgcc
     4201 catctactct ccctgttgtc tggctgacgg cagtttggcc gctttattaa catcatacca
     4261 ccaggtatcg atacccagac tgtaaacagg gcgcacagcg ggttgggaga atttatccca
     4321 ccaggcgaga cggtcttccg ccatgtacca cattggcagc atgtaataat tccacgttaa
     4381 tacgcgatcc agtgctcgcc ctaacggcag taatttttct ttatttccct gcgcggcaat
     4441 aatttggttg atcagcgagt cgataatccg gctttgcacg ccgggggcat tataagtgga
     4501 attgatatat tccgatgacc aggaaatctg taaatcggaa ctgggccacg gcatcgcccg
     4561 ccataggcgc ggcatcatgt catagtcgcg actgcgcatg cggttggtga tttgcgagtt
     4621 atccaccttg cgaatgtcca tgttgatacc cagccgttgc aggctgtgct ggaacggcaa
     4681 tacccactga ctattgctgc ttgcaggaag caataattca aagctgagcg gctgacccgt
     4741 agtggcatta acgcgttgct gacctttcag cacccagccc gcttcgttga gaagtttgtc
     4801 ggcttttaac aggttgtcac gatcgtagcc atcgcctttg gataccggcg gctggtagat
     4861 ttgtgtgaag acttctggcg gtagatcttt tttcattggt gccagaagca ccagctccgc
     4921 ggcgtcgggg taatttctgg ccgcgtattc ggtattctga aaataactgt tcgtgcgact
     4981 ccaggcattg taaaacaacg ccttgttcat ccattcaaag tcaaaggcga gagtgattgc
     5041 ttcccgcacc cggcgatcgc tgaatactgg acgttggata ttaaacgcca gccagcgcgt
     5101 atcctgggct gattcgttct tttgctcatc tttgatgatg tattttttat cgaaattttt
     5161 accggtataa cgcgtggccc agtttttagc gtcgttttcc atacgcaaat caaaggcacc
     5221 tgctttaaac gcttcaaagg cgacattatc atcgaggtaa taatcgtagc gaatggtgtc
     5281 gaaattccag cgtccacggt ttaccggtaa gtttgctgcc cagtaatctt tcacgcggga
     5341 atagacaata ttttgcccca ttttccagga cgtaattcgg tacggaccac tggcaagcgg
     5401 aggcgtggcg agcggatcgc taagtttgtg atccttccag tacttttctg gaaataccgg
     5461 cagcgaaaac agactcaaca tatcttcttt gccgggttta gctaactcaa tgcgcacggt
     5521 taacggcgca atggctttga cggtggtgcc tttgtagacc agacgaaatt gcggcacgcc
     5581 ttcagtcata aatttttgaa aagtaaactc tacatcgcgg gcagtaatgg gcgaaccatc
     5641 atgaaaacgg gcgcgcggat taatagcgac ctccacccag gaataatcgt cagcatagcg
     5701 tgcgctttca gcaatcagcg ggtaataact gcctggttcg tcatcggaag tcgtaaatag
     5761 cgtgtcgtac agctgctcgg tgcgtgcgcc cgggttgccg cgcagtgcat agcggttgaa
     5821 attatcgaag gtacctaggg ctgacaacgt tatctgccca ccttttggcg cggcggggtt
     5881 cacataatca aaatggttga aattaaacgc gtaccggggt tcgcccagca cggcaaaggc
     5941 atagctttcc ttgatagcct gcgcctgcgc accaaaggtg aacagagcga taaacagcag
     6001 cagtatgcgc acaatcatct gcaacggatt tccttttact catcattagc cgcaatccgg
     6061 catgctttca ctatgaataa taagggacgt aagcacacct taccactgcg gcgtatacgg
     6121 tttcttcagc cagcggacaa aatcgtccag cggtaacggg cgactaatcc agtagccttg
     6181 catgaaatta acgccgcgtt cgcttagcca tcgcgcctgt tccggcgttt cgaccccttc
     6241 agcaaccgtc agcatattga ggcttttcgc cagcgtcagc accgcgtcaa gtacgggtga
     6301 agtgatcgtt tccgtaccga tggcgttgat aaatccacgg tcaattttca gataatcgag
     6361 cgtaaaacgc tcaagataga taagcgcgct gtgcccggtg ccgaagtcat caatagcaat
     6421 ttctatgccg accgagtgca gccaggcgaa gagttgtgtg gcttcttgct ctttcagcat
     6481 atcgtgctcg gtaatttcca gcacaatctg gaaatggtgt gcgggcaggg aagtgagcag
     6541 tttctggata tctgctttaa agctttcgct gtgcagatgg tccggcgcaa tgttaatacc
     6601 aaatttgacg cctaccggca gcactttttc taattctgcg gcatcgcggg caattaactc
     6661 aaacaggtgc tgagtcagcg gcacaatcat cttttgagat tcggcaaagt taatgaaggc
     6721 atccggggga atttctcccg cgacaggatg ccgccagcgt agcagtactt ccaggcccgt
     6781 tactcgcaaa gcttgtgtat ccaccaccgg ttgatacgcc acgtaaaatt gttcgcgctt
     6841 gatggcggtc atgatttctc tgccggggcg catacgtacg ctcatcaggt aatagcagag
     6901 caggccaacg acagttccac tcatgccgcc cagtaaaaat gcgtaccaca catcgttcca
     6961 tgtccagtca tctgcataaa gccgaacggt cagaggaatg cccgcaattt tagtttcacg
     7021 gactggcatg tcggttaatt cgttaacgtt catcaaacgt gaagaaaagg tagatagcgc
     7081 agtattgcca ataatgaggg cgacgccatc gtaatcttcc tgccgtgaac tataaaagag
     7141 tgaaggcgtc aggttgagat tcagagcggc aaagacgccg ctatttttca gcaaagggtt
     7201 gcgataccag atgacgattg cgggtttgtt cggcaccatc ggcgtgccgg gtaagatcgc
     7261 catatcgaca tttttattaa tgtcgagcgc cggaatcaat tcattgagtg gaatgtccat
     7321 ctcaccggtc gcagatgaac agaatgtttt tttatctttc accagcacaa acgtacggac
     7381 attcatgcta aacgccgcgc gggcggtcag ttcggggtta gcctgctggc aggtatctaa
     7441 ggtcagcggc tgaagccggt cagtagtgga tttcaggtcg gcaaaatagg tatcgagata
     7501 cttttgtacg tcggtaatca gtgtgtcgta tttgacttcg tgcttatgcc aggccaccaa
     7561 aaactgaagg cagctcacca gtatcgcaat catcacgcct gcaacaatgc aggtaagcag
     7621 gagcttacgt ccagaattgg gagcgcgtat gaacatgatt ccgttaattt cctgaaatta
     7681 cgacaacatt taaaagataa caatgaccgg actatagcct ggatcaagac gcggaaacat
     7741 cattttctct gatgaatgaa aacaaaaaag cactgcctaa gcagtgcttt cagttaactc
     7801 gtcaggatag ccaagggatt gcatccaaac ggtttattag ctgcggctga gaacccggcg
     7861 tgcttcgttg taacgcttct tccagtacgg ttcattcatg ctggaaataa taacaccact
     7921 gctggtggaa gcatggacaa actgattgtt gccgatataa ataccgacat ggcgtcccgt
     7981 tgaaccggca cggaacagaa ctaaatcacc cgtacgcaaa ttactgcggg aaacagattt
     8041 acccatttcc tgctgttcgt aagtcgaacg cggaagttct aagccaaatt gctcacggaa
     8101 tgtacgctgt acaaaaccag aacaatcgat acctttttta gtgctgccgc ccagacgata
     8161 acgtacgcct ttccagtcag catactgatc cataattcgc gattttacgt cgacattacg
     8221 aaccaggttt tcaaattcat cctgagaagc ttgcagtgat gatgtttcac tacccactgc
     8281 acgtgtctca ggatgcatat tctttgcggt gttatttgca ctacatgcag aaagcagaac
     8341 cgctactgca atcgcgggga tcccgcgcaa gatatatctc aaaatcggtt gagatttgac
     8401 catgttgttt gttttccctt gaagtcctta acgacaaata tcgttataaa aaaatgccaa
     8461 gcgacacgag actaattacc tgggcatgtt gaaacaattc cttaaggcca aatctgtgga
     8521 ctaacgcaca aaattatatg ctgtgaggat aatctaccgc cgaggcaaaa cgagttcgag
     8581 attacccgat tggtgcggtc ttggcgagcg gttttacatg attttttata agaaaaagtg
     8641 atacagaaag ttaataagcg ggattggtca attaaagtac gattgagtga aacgcatgca
     8701 atccattcat ttacttagag aatgattttt gctttcgatg gatgaaagca cggtgcgcat
     8761 catcaggatt atcctcacta taaaaataaa cctgatgatg ttaattactg tgagttattt
     8821 gttttggaaa tgtttgtttt ttcccggtag tgatttgtca aaaaaggtga ttaatcgatc
     8881 acttaatggc gtcagcaaca cccagggcaa accgatcaat atcacggtca tcgaaccgac
     8941 aatgatgtca gtaaaccagt gtgcgccaat cattactctg ggaaacgcaa aaaccacaaa
     9001 aataataagg gcgataagac ctgcaacttt gccgaaataa cgccacatga atgacgaaaa
     9061 aataagcagc atcatgccgt gatcgccggg aaagctatct cgtgaggcat ctttcgtggg
     9121 aacagagagc agttcgctga cgcggttaat atcggtaaaa gtcaatgttg ggctggcccg
     9181 ttttacagga attaatgcct gacccagctg gtttaatacc actgcagtta atagcatgac
     9241 cagaccaata atcacgatac gtcgtctgcc tggggcgttt tctttcagcc agaaactcag
     9301 catcaacatt cccatcgcca gcagtgaaca accgtcgaaa gcgcgattgt tggtcaatgc
     9361 aaccagccac aaaaaggcct tactttcgac cagtttctga ttaaagaaat aaaaaatatc
     9421 cgcatcaatc ggcaaccaga atccatgatt aacgggaata taccaggaaa gaaacagcgc
     9481 gaggccgaca atattcaaca acactatttg cggcaaattt ttaatcatat tttctctaat
     9541 tatcttactg aaaaacgcag gcaaccttac gcggtagtcg ctaaacgaag cttcaacagc
     9601 gcgctctgca aggcattcca gtcagcttcg ctgctggaaa tcagctcaat acggctgtcc
     9661 ggtggcgcaa cgttttgcgt ttcaatgtgc aggtcatcgc cctgacggtt gattcgcacc
     9721 agcccttcgg gaatacgcag cacgcctttg acgcgttcca ccggtgcaag tcgcgcccat
     9781 tccagaatgc caatggtgtc gaataccgta tcagcgtcga atatccagcc gcaggcctga
     9841 tgtccttgtc cactgttcag actgcgacgc cagcgttgat gctctggcag gcttaacgct
     9901 gctaaccctt ttttcacgac atgctgatga gaatgcgcgg cgctggcggg caactcggct
     9961 aaattgcgac gcggcaaatc cagaagatga ccgtcaactt tcccatgctc actgtgaatt
    10021 aattgccgat cgccaccatt ttgctgccac cagcgctgta gcgcttgctc actttcaggc
    10081 gtcgcacggt cggatttatt ggcgacaatg atgtctgcgg cagccagctg gtcacggaag
    10141 ttttcattgc tggcgctttt ttcgtccagc agcaggcgtg gatcgagaat gcacaacgtg
    10201 gcgcgcagat ctatccacgg ttcatagact ggtgcggtta acagatcgag gatctgtttc
    10261 ggatggccca gcccggtcgg ctctatcaac aagcggtctg gttttccctg acgcagtaag
    10321 gtattcaacc ctacctgcat gggtaaacca ttaacgcagc acatgcagcc gccggggatc
    10381 tctttcagca atgcgccgct atcggcgagc aaagcaccat caattccgac ttccccaaat
    10441 tcattaacca gtaccgccca tttttcgttg ggatctttat gggctaacag atgaagaatc
    10501 gacgtagttt tcccgctgcc gagaaaaccg gtgatgaggt tggtcctggt cacaacggct
    10561 ccgcaatact ctgataagtt aactgagcgt taatcctggc taaaaacggc agaaaagaga
    10621 aggggagagc ggacgaaggc gagcttcatc cgctttaacc gcagaaatcg ttaaattttg
    10681 agggtgttta atagcgcctg atgcgcgccg aattgtgcga tttgttgcca ggcttgtttg
    10741 atggcctgca caaaatgcgg gttatctggc agatcatcac cgaaaacttc acgcagggaa
    10801 agcagggcgg tgacgcgttg ttcactgctg ctgcccgcaa caagttcgcg aattttatcg
    10861 ctaagcggat cgcgaacatc aatggcattt ccggcatcat caacgccgct gacgtaacgc
    10921 atccagcctg caacgcctaa tgccagcaac gaccagtccg tttcgcgccc cagatgtatg
    10981 cgaatacctg ccagcatgcg ttgcggtaat ttctggctgc catccatcgc gatttgccag
    11041 gtcttatgtt tcagcgccgg attagcaaaa cgtgcaatta acttatccgc atattgtgtt
    11101 aaatcgacat ctttaattcg cagtgtcggc gcttgctcat tcagcattaa tgttctggcg
    11161 gcatggcgaa atgcgcgatc ctgcatgcaa tcactgatgt gggcgaatcc tgagaggtaa
    11221 cccagataag cgagaaaaga gtggctgcca ttaagcatcc gcagtttcat ctcttcccat
    11281 ggcaggacat cattcaccat ttgtacacct gcgacttccc aggcaggacg cccagcgacg
    11341 aagttatctt ccaccaccca ctggataaac ggttcgcagc taatcgcgca gggatcattc
    11401 acccccagat gctggctgat ttccgccagt gattcgtcgg ttgcagccgg aacaatgcgg
    11461 tcgaccatgg ttcccggaaa actgacgtgt tcttttatcc acccggcgag ttctggcgaa
    11521 cgtttttctg ccattcccag caccgcgttt ttcaccacat ggccattgtc gggaatattg
    11581 tcgcaggaga gcacggtaaa cggtgtaagg ccgcgctcac ggcggcgttt cagtgcttcg
    11641 acgagaatac ctggcgcgga gtgaggttct tcaggggttt gtagatcgtg aataatccgc
    11701 ggattgctgg tgtcgagtgc accggtggcc gggtcaatac aatagccttt ttcggtaatc
    11761 gtcagggaaa caattgccac ctgtggctcg caaaatttct caataattgc cgctaaggaa
    11821 tccagttttg cattcaggca ttcgtgaacg gcaccgacaa ttatcgcctg attgccgtcc
    11881 gcccctttct ccagcacggt gtataaatgg ttctgtgcgc ggagctggct catcagttga
    11941 tcaccgctga acaagctgat ttcacagatc ccccagtcgc cgccctgggc attcagcaca
    12001 cgatcggtca gtaacgcctg atgtgcgcgg tgaaaggctc caaagccaaa atgaacgata
    12061 cgtgattgca actgctggcg atcgtagcgt ggagcgtgta catgatgcgg gagcgtaacg
    12121 gaggcgattg tattcatagg agtcacgtct tagtaacgat taattaacaa tgacagtgta
    12181 aagttatatg acagcaattg aaattggtct ggattgttaa tgcgatgaag tgtgagggcg
    12241 atcaatcaac gagtgaaaat tgagggcgta aggtgttcag gaagcgcaaa atagaagatt
    12301 ggtataataa attaaaaaag ggacagcctg agctatccct tttctgcatg cggctgaagt
    12361 tagtcagcac gccccatata acggcgttct tcgatatgga tacgaatttt ttcgcccggg
    12421 cttaagtatt ctggtacctg aatcaccaga ccagtgctca atgtcgccgg tttgttacgg
    12481 gcgctggcag atgccccttt gatgcctggt gcggtttcaa cgatttccag atcaacggtc
    12541 tgcggcagct caagcgccag cagttgaccg tcccaggtca gcacctgcat gtccggcatg
    12601 ccgccctctg gcataaacag caactcttct tcaatctgat ctttggtgaa ggtatacggg
    12661 gtatagtctt ctttatccat aaagacatat tcgttgccat cgacatagga gaagtcaacg
    12721 taacggcggg tcagcgtcac ggtgtcaacg atatcatctc ctttgaaacg ctcttctact
    12781 ttcagcccgg tacggacatc agaaaaacgc attttgtaca gcgtagcggc accgcgggca
    12841 gtgggcgact gaatatcaat gtccttaacc agcagcagtt tgccgttgta attcagtacc
    12901 ataccttttt taatttcgtt cgctcttggc atcgaaaaat tcctgtagtc gaaaatgtca
    12961 aaaatatcgc gacaaagtac tcgcgacggg cctttcgggc aagtggtatt cgcacttttg
    13021 ctggtgcaaa aaaggtggta ctgtgcgcgc tcatcaattc ggtggttaac cttaagagaa
    13081 caacgtatgg aatgccgtcc gggttgcggg gcgtgttgca ctgccccttc aatttccagc
    13141 cctattcccg gtatgccaga tggcaagccc gccaatacgc catgcattca gcttgatgaa
    13201 cagcaacgct gtaaagtttt cacctcgcca ctgcggccga aagtctgtgc aggcttacaa
    13261 gccagcgctg aaatgtgcgg taactcgcgt cagcaggcaa tgacctggct tatcgatctg
    13321 gaaatgctga ccgcgcctta aacatcttta atccgcagta agcaaaacag agtagcgata
    13381 atcatcacca tcgcaaacca gaacacagcg tgataattcc agatctcggc gacgatgccc
    13441 gccactgatc ctgcgatgat ccagcccacg cgcgacgtgt tggtatagag cgtggtggct
    13501 gaacctgcct gaccgggcat caaatcctga aaatagagca tcccgatgcc gcccagaatg
    13561 ccaataaaaa tagcatttag cagctgcaag cccaacagaa tggcaggtga atgcgccatc
    13621 agcattcctg cgtaaaaaca gacgccaccc acggcggcaa cgcgcattaa gaagcgctta
    13681 cccagacgtt tggcaaaata tccggcaatc aacatggtcg ggatttccag cccggcggcg
    13741 gtccccatca tcacaccggc cagtttctcg ggaagatgca gttcgttgat aataaatagc
    13801 ggcatgttga tgatgtacag gctgttcgag ccccacatca atgtacaaat gacaaacagc
    13861 agcagcgtat cgcgacggtt acggcgcggc gcttcaaccg tgccggtcgc cagcggaagc
    13921 tctttttgca tcgacggtaa aaacagccac accatcacgc cgcaaacaat aaatgctacc
    13981 gctgcgctca gatacattac cgtaaagctg aaacccatcg ctaaggcata agccagcggt
    14041 gggccaatga cccatgccag tgaaacctga gcgcgtaaaa aagagctgaa catcaccgcc
    14101 tcacgtccgg ttttgtcggc atgttcacgg gcaagggcaa acatttgcgg gttagcggtc
    14161 gagccaaagc tgctaagaaa gacgccaacg aatagcaaaa caaagtagtt gcgattccag
    14221 gcaaaaaggg tgcaggccag cacgcctaac aggcagcaaa agacaatcag cgatttgcga
    14281 tcgccgcgct tatcagagcg cccggcgaga aactgactca ccagaatccc aatgacagcg
    14341 ctgccggtaa agaagaatcc caccatcgcc ggacgggcat gtacttcatc ggtaagaaaa
    14401 atactgagtg tcggggtttg cagagcgccc gcaataccgg tgagaaaggc aacgattaaa
    14461 aacgccgtcg aggtcaggtc aaacgatttc gcgctggaga ctgcggggga gttatgcatg
    14521 ctggtcgatc actggaagag gaagacgcgg agtttacgct gcttgtcaga aaataaacag
    14581 acagcgaaat caaaagcgcc acgaataatc aaaatgccat ttcactgttg ctgattggct
    14641 gctgacgctg ctgaaatgaa attgctgata acggattttc ccatcagcaa ttcggaaaaa
    14701 tgacaaaaaa ttgtgcagca catcaaactt ttgctcataa ctttacggct ttccttgcgt
    14761 gctgaaacag aagtattatg ctttcttgaa acgtttcagc gcgatcttgt ctttaaccct
    14821 aagccaggtt ggcgcttttt ttctcataga agctgaatcg tttcaattca gcaagagagg
    14881 agaactatgt tccagttatc cgtacaggac atccatccgg gcgaaaaggc cggagacaaa
    14941 gaagaggcga ttcgccaggt cgctgcggcg ctggtgcagg ccggtaatgt agcagaaggc
    15001 tacgtcaatg gcatgctggc gcgcgaacag caaacctcaa cgttcctcgg caatggtatt
    15061 gctattccac acggcactac cgacacccgc gatcaggtgc tgaaaaccgg cgttcaggta
    15121 tttcagttcc cggaaggcgt cacctggggt gacggtcagg tagcgtacgt agcgatcggt
    15181 attgctgcca gctcggatga gcatctgggc ctgctacgcc agctgaccca cgtactgagc
    15241 gatgattccg ttgctgaaca actgaagtca gcaacaacag cagaagaact tcgcgcattg
    15301 ctgatgggcg aaaagcagag tgagcagctg aagctcgaca acgaaatgct gacgctggat
    15361 atcgtcgcca gcgatctgct gactcttcag gcgctgaacg ctgcgcgtct gaaagaagcg
    15421 ggggcagttg acgccacttt cgtcaccaaa gccatcaatg aacaaccgct gaacctcgga
    15481 cagggcatct ggctgagcga tagcgccgaa ggcaatttgc gtagcgcgat tgctgtaagc
    15541 cgtgcggcaa atgcttttga tgtggacggc gaaacggcag ccatgctggt gagtgtggcg
    15601 atgaatgacg atcagcccat tgcggttctt aagcgtctcg ctgatttgtt gctcgacaat
    15661 aaagctgacc gcttgctgaa agcggatgcg gcaacgttgc tggcgctgct gaccagcgat
    15721 gatgcgccga ccgacgacgt gttaagcgcg gagtttgtgg tgcgcaatga acacggcctg
    15781 catgctcgtc caggtaccat gctggtcaac accattaaac aatttaacag tgatattacc
    15841 gtgacaaacc ttgatggcac cggcaaaccg gcaaacggac gtagtctgat gaaagttgtg
    15901 gcacttggcg ttaagaaagg tcatcgccta cgctttaccg cccagggtgc agatgctgaa
    15961 caggcgctga aagcaatcgg cgacgctatc gctgctggtc ttggggaggg cgcataatga
    16021 gcagacgtgt tgctactatc acccttaatc cggcttatga ccttgttggt ttctgcccgg
    16081 aaattgaacg cggcgaagtg aacctggtga aaaccaccgg tctgcatgcg gcgggtaaag
    16141 gcatcaacgt ggccaaagta ttaaaagacc tgggaattga tgtcaccgtt ggcggcttcc
    16201 tcggtaaaga caatcaggat ggttttcagc aactgttcag cgagctgggc attgccaacc
    16261 gtttccaggt tgtacagggg cgcacccgaa ttaacgttaa gctgacggaa aaagacggcg
    16321 aagtgaccga cttcaacttc tcgggttttg aagtcactcc cgccgactgg gaacgctttg
    16381 tgactgattc tctgagctgg ctcggtcagt tcgatatggt ctgtgtcagc ggaagcttac
    16441 cgtcaggcgt cagcccggaa gcgttcaccg actggatgac tcgcctgcgt agtcagtgcc
    16501 cttgcattat ctttgatagt agccgtgaag cgttagtagc aggtttgaaa gcggcaccgt
    16561 ggctggtgaa acctaaccgc cgcgagctgg aaatctgggc aggccgtaaa ctgcctgaaa
    16621 tgaaagatgt gattgaagct gcgcatgcgc tgcgtgaaca aggtattgcg catgtcgtta
    16681 tttcactggg tgccgaaggc gcgctttggg ttaatgcctc cggcgaatgg atcgccaaac
    16741 caccgtcagt cgatgtcgta agcaccgttg gcgcagggga ttctatggtt ggtggcctga
    16801 tttatggctt gctaatgcgt gaatccagtg aacacacact gcgtctggcg actgctgttg
    16861 cagccctggc ggtaagtcaa agcaatgtgg gtattaccga tcgtccgcag ttggccgcaa
    16921 tgatggcgcg cgtcgactta caacctttta actgacagca ggagaggcat aatgaaaacg
    16981 ctgctgatta ttgacgctaa tctcggtcag gcacgcgcct atatggcgaa gaccctgctg
    17041 ggcgcggcgg cgcgaaaagc aaaactggaa atcatcgaca atccgaacga cgcagaaatg
    17101 gcgattgttc tcggtgattc catcccgaat gacagcgcgc tgaacggtaa aaatgtctgg
    17161 ctgggcgata tttcccgggc agttgcgcac cctgagctgt tcctgagtga agccaaaggc
    17221 catgcgaaac cttacactgc gccggtcgct gcgacagcac cggttgccgc cagcggtccg
    17281 aaacgcgtag ttgcggtgac tgcttgcccg actggcgtag cacacacctt tatggcggct
    17341 gaagccattg aaaccgaagc gaaaaaacgt ggctggtggg tgaaagttga aacccgtggt
    17401 tctgttggtg cgggtaatgc aatcaccccc gaagaagtag ccgcagcgga tctggtgatt
    17461 gtggcggcag atatcgaagt ggatctggcg aaatttgctg gtaaaccgat gtatcgtacc
    17521 tctaccggtc tggcgctgaa gaaaaccgcg caggaactgg ataaagcggt tgctgaagca
    17581 acgccgtatg aaccggcggg caaagctcaa acggcgacct ctgaaggtaa gaaagagagt
    17641 gcaggcgcat accgtcactt gctgacgggc gtctcttaca tgctgccgat ggtcgttgca
    17701 ggtggtctgt gtatcgcgct ttcttttgct tttggtatcg aagcgtttaa agagccgggt
    17761 acgttggcag cggcgctgat gcagattggt ggtggttcag cctttgcgct gatggtgccg
    17821 gtactggcag gttacattgc cttctccatt gccgatcgtc cgggtctcac gccgggtctg
    17881 attggcggta tgctggcggt cagcaccggt tctggcttca ttggcggtat tattgcgggc
    17941 ttcctggctg gttacattgc gaagttaatc agtacgcaac tgaaactgcc acagagtatg
    18001 gaggcgctga aaccgatcct gatcattccg ctaatttcca gtctggtggt cggtctggcg
    18061 atgatctacc tgatcggtaa accggttgct ggcattctcg aagggttgac tcactggctg
    18121 cagaccatgg ggactgcgaa tgcggttctg ctgggggcga tcctcggtgg catgatgtgt
    18181 actgacatgg gcggtccggt aaataaagca gcgtacgcat tcggtgtggg tctgctgagt
    18241 actcaaacct atggcccgat ggcggcgatt atggcggcag gtatggtgcc accgctggca
    18301 atgggtctgg caacaatggt ggcgcgtcgc aaattcgaca aagcgcagca ggaaggtggc
    18361 aaagccgctc tggttcttgg tctgtgcttt atttcggaag gtgcaattcc gtttgctgct
    18421 cgtgatccga tgcgtgtgct gccgtgctgt atcgtgggtg gggcgctgac tggcgcaatc
    18481 tcaatggcga ttggtgcgaa actgatggca ccgcacggtg gtctgtttgt tctgctgatc
    18541 cctggcgcta ttacgccggt actgggttac ctggtagcaa ttattgccgg tacgctggtg
    18601 gcgggtttgg cctatgcctt cctgaaacgt ccggaagtgg acgcagtagc gaaagccgcg
    18661 taataaaagg tgtgttacag ggcagaaatt tactgccctg tatcattgga tgagcgcaaa
    18721 gagaatgacc ggtttgtgaa tgtaatttta tgaatttaaa tggttttatc tttcagtaga
    18781 aacctgaaac gttcatcaaa cactaaggtt atttcctcaa gggcagattc ttttctgccc
    18841 ttttttagtg ttcatgttct gtgcatcccg tcacaaattc ctcgcttttg taattcaggc
    18901 atttaatcgc attttgtgag cctgctcaaa tctgcccgct ttgcctctct ggcggcaatt
    18961 tactttacgt aaaacatgca tactatgagc acatgtttaa atatgaacac atgtttaaac
    19021 cgtctttgca gtatctatat ccttgtggat tgacagggtt ggtaattttt taccagagga
    19081 cgtgctatgc gcgaaaagga ttatgtcgta attataggtt cggcgaatat tgatgtcgcc
    19141 ggatattcac atgaatcatt aaattatgcg gattcaaatc caggtaaaat aaaatttaca
    19201 cctggtggag tagggcgaaa tattgcacaa aacctggcgt tgctgggtaa caaagcctgg
    19261 ttactgagcg cagtaggtag tgatttttat ggtcaatcgc tgctaacgca aaccaatcaa
    19321 tctggcgttt atgtcgataa atgcctgatt gtgccgggag aaaatacgtc gagttattta
    19381 tcattactcg ataataccgg tgaaatgctg gttgctataa atgacatgaa tattagcaac
    19441 gctattacag ctgaatatct cgcacagcac cgtgaattta ttcagagggc aaaggtcatt
    19501 gtcgcggact gtaatatcag tgaagaggca ctggcatgga ttctggataa tgctgccaac
    19561 gtacccgtat ttgtcgatcc ggtttcagcg tggaaatgtg tcaaagtgcg cgaccgtcta
    19621 aatcagatcc acactctcaa gccaaaccgc cttgaagcgg aaaccctgag tgggattgcg
    19681 ctgtcagggc gtgaagatgt ggcaaaggtt gccgcctggt tccatcaaca tggcctgaac
    19741 cgactggtat tgagcatggg cggcgacggc gtttattaca gtgatatcag cggtgaaagt
    19801 ggttggtctg cgccgatcaa aaccaatgtt attaatgtta ccggagcggg cgatgccatg
    19861 atggcgggac ttgcttcgtg ttgggtagac ggaatgccgt ttgccgaatc tgttcgtttc
    19921 gcacagggat gttcgtcaat ggcgctctcc tgtgaataca ccaataaccc cgatttatcg
    19981 attgccaacg ttatatcgtt agtggagaac gcagaatgtc tgaattaaaa atttcccctg
    20041 aattattaca aatttccccg gaagtgcagg acgctttaaa aaacaaaaaa ccggttgtgg
    20101 cgctggaatc gaccattatt tctcacggga tgccgttccc acaaaatgcc cagaccgcaa
    20161 ttgaagtaga agaaactatt cgtaaacagg gcgcagtacc tgccactatc gccattattg
    20221 gcggcgtgat gaaagtgggt ttaagcaaag aagaaattga attactgggt cgtgaagggc
    20281 ataacgtgac caaagttagt cgtcgcgatt taccttttgt tgttgccgcc ggaaaaaatg
    20341 gcgcaaccac tgtggcttca acgatgatta ttgcggcgct tgccggaatt aaagtatttg
    20401 ccaccggcgg aattggtggt gttcatcgcg gggcggaaca taccttcgat atttctgccg
    20461 atttgcaaga actggcaaat actaatgtca ccgttgtttg tgccggggcg aaatctattc
    20521 tcgatttagg attaaccact gagtatttag aaaccttcgg tgtgccgtta attggctatc
    20581 agactaaagc gctgcctgcg tttttctgcc gcaccagccc atttgaagtc agtattcgcc
    20641 ttgatagtgc cacggaaatc gctcgtgcaa tggcagtgaa atggcaaacc ggtttgaacg
    20701 gtggtctggt agtggcgaat ccgatcccgg aacagtttgc gatgccggag gaatctatca
    20761 atgcagccat agatcaagcc gtcgccgaag ccgaagagca gggcgttatt ggtaaagaaa
    20821 gtacaccgtt cctgctggcg cgtgttgctg aactgaccgg cggtgacagc ctgaaatcca
    20881 acatccaact ggtgttcaac aacgccattc tggcgagcga aattgccaaa gaataccagc
    20941 gtctcgcggg ttaatttctg acgggcagaa agccgtcgcc cgtactgatc atacataccg
    21001 aaacctggcg ctgacgccgg gtttactggc atgaagggaa aatcattatg gatataatga
    21061 gaagtgttgt ggggatggtg gtgttactgg caatagcatt tctgttgtcg gtaaataaaa
    21121 agagcatcag tttgcgcacg gttggagccg cactgctgct gcaaatcgct attggtggca
    21181 tcatgctcta ttttccgccg ggaaaatggg cagtagaaca ggcggcatta ggcgttcata
    21241 aagtgatgtc ttacagtgat gccggtagcg ccttcatttt tggttcgctg gtcgggccga
    21301 aaatggatgt cttgttcgac ggtgcaggct ttatcttcgc ctttcgcgta cttccggcga
    21361 ttatcttcgt cactgcgctt atcagcctgc tgtactacat tggcgtaatg ggactgctaa
    21421 ttcgtattct tggcagcatt ttccagaaag ctcttaacat cagcaaaatc gagtcttttg
    21481 ttgcggttac cactattttc ctcgggcaga acgagatccc ggcgatcgtt aaaccgttta
    21541 tcgatcgcat gaatcgcaac gagctgttta ccgcaatttg tagcgggatg gcatccattg
    21601 ccggttcgat gatgattggt tacgccggaa tgggcgtgcc gattgactac ctgttagcgg
    21661 catcgctgat ggctatccca ggcggtattt tgtttgcacg tattcttagc ccagcaaccg
    21721 agccttcgca ggtcacgttt gaaaatctgt cgttcagcga aatgccgcca aaaagcttta
    21781 tcgaagcagc ggcgagcggt gcgatgaccg ggctgaaaat cgccgccggt gtggcgacgg
    21841 tggtcatggc gtttgtcgca attattgcgc tgattaatgg cattattggc ggaattggcg
    21901 gctggtttgg ttttgccaat gcctctctgg aaagtatttt tggctatgtg ctggcaccgc
    21961 tggcgtggat catgggtgtg gactggagtg atgccaacct tgcgggtagc ctgattgggc
    22021 agaaactggc gattaacgaa ttcgtcgctt acctgagttt ctccccatac ctgcaaacgg
    22081 gcggcacgct ggaagtgaaa accattgcga ttatctcctt tgcgctttgt ggttttgcta
    22141 actttggttc tatcggtgtt gtcgttgggg cattttcggc tatttcgcca aaacgtgctc
    22201 cggaaatcgc ccagcttggt ttacgggcgc tggcagcagc aacgctttcc aacctgatga
    22261 gcgcgaccat tgccgggttc tttattggtt tagcttgagt ttcattgccg gatgtactcg
    22321 cttattaagc gttgcatccg gcaatcgcac tattgcaaat aatttttact gcatcatccc
    22381 ggagaattta ttctccgggt ccatctccag cgccagccct gacaactgct ttctgttttt
    22441 aattagatat cctttcttgc ttttaattaa taaaccgtcg tgaatgaatt gcgcgagaac
    22501 atataaaaga tgtcgataag aaacgcctaa atactctgct gcctgggtat gtttttcgtg
    22561 ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca ctaacggaaa
    22621 tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta aggtgacgca
    22681 aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt tcatagggag
    22741 cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt ctttatcgat
    22801 taactcaatc tcgccaataa aacagggcgc ggcaaagaaa tcgatcagcg acacgcgacc
    22861 attagctagc gtggcgtaaa gcctggcgcg gcctcgcgtc aggtaaaaca gccaggacgg
    22921 ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc gcgtatcggc
    22981 tgaaacatcc gttaaaaagc aatccttaaa cgcggattca ctcatcaatt gcagcttaag
    23041 atcattattg tggatttctt tcacgatgtt tatccgctgt gagatttctc atatttagca
    23101 tacgtacgct gttgttaaga ttcaatcatc ggcccggtcg aattttcttt gctgtagaaa
    23161 aaataacgca actggaaaca gaggaaataa acaatggaaa agagaaaaat tattctggat
    23221 tgtgatccgg gtcatgatga tgctattgca atgatgatgg cagcgaaaca tccggcaata
    23281 gatttattag gcatcactat tgtagcgggt aatcagacgc ttgataaaac attaattaat
    23341 ggcctgaatg tttgccagaa actggagatt aatgttccgg tttatgcggg gatgccgcag
    23401 cccattatgc gtcaacaaat cgttgccgat aatattcacg gtgaaaccgg actggatggc
    23461 ccggtattcg agccgctgac ccgccaggca gaaagcactc atgcggtgaa atacatcatt
    23521 gataccctga tggcaagcga tggcgatatc actctggtgc cggttggtcc gctttcaaat
    23581 atcgcggtgg caatgcgtat gcaacccgcg atcctgccca aaatccgtga aattgtgcta
    23641 atgggcggcg cttacggtac aggcaacttc acgccatctg ccgagttcaa catctttgct
    23701 gacccggaag ccgcacgcgt ggtgttcacc tccggcgttc cattagtgat gatgggcctc
    23761 gatcttacca accagaccgt ttgcaccccg gacgtaattg ctcggatgga aagggcaggc
    23821 ggccctgccg gagagctatt cagcgacatc atgaacttca ctctcaaaac gcagttcgaa
    23881 aactacggcc tggctggcgg cccggtgcac gacgccacct gcatcggtta tctgattaac
    23941 cctgatggca ttaaaaccca ggagatgtac gtcgaagtgg acgtcaacag tggcccttgc
    24001 tatgggcgta ccgtctgcga cgagctgggc gttcttggca agcccgctaa taccaaagtc
    24061 ggcatcacta ttgataccga ctggttctgg ggcttagtcg aagagtgcgt gcgcggctac
    24121 atcaaaaccc attaagtgtt gataacaagc cgggtcagta tcgcaagatg caggcccggc
    24181 tatttctgag gggcagaaag ccgtcgccca atatgacatg aagagataat aactatggat
    24241 gtcatgagaa gtgttctggg aatggtggta ttgctgacga ttgcgttttt actgtcagta
    24301 aacaagaaga agatcagcct gcgtaccgtt ggcgcggcgc tagtgttaca ggttgtgatt
    24361 ggcgggatta tgctttggtt accgccaggg cgttgggtcg ctgaaaaagt cgcttttggc
    24421 gtgcataaag tgatggcgta cagcgatgca ggtagcgcat ttatcttcgg ttcgctggtc
    24481 gggccgaaaa tggacaccct gtttgatggc gcaggattta tctttggttt cagggtgtta
    24541 ccggcaatta tcttcgtcac cgcgctggtg agtattctct attacatcgg tgtgatgggg
    24601 atcttaattc gcattctcgg cggtatattc cagaaagcat taaatatcag caagatcgag
    24661 tcattcgtcg cggtcactac cattttcctc gggcaaaacg aaattccggc gatcgtcaaa
    24721 ccctttatcg atcgtctgaa tcgcaatgaa ttatttacag cgatttgtag tggcatggcc
    24781 tcgattgctg gttcgacaat gattggttac gccgcactgg gcgtacctgt ggaatatttg
    24841 ctggcggcat cgttaatggc gatcccaggc ggaatcttgt ttgcccgcct gttaagcccg
    24901 gctacggaat cttcgcaggt ttcttttaat aacctctctt tcaccgaaac accgccaaaa
    24961 agcattattg aagcggcagc gacaggggcg atgaccgggc tgaaaatcgc tgcgggtgtg
    25021 gcaacagtgg tgatggcatt tgttgccatc attgcgttaa ttaacggtat tatcggcggc
    25081 gttggcggct ggtttggttt tgaacatgcc tcgctggagt ccattttagg ttacctgctg
    25141 gccccactgg cgtgggtaat gggtgttgac tggagtgatg cgaatcttgc cgggagtttg
    25201 attggacaga aactggcaat aaatgaattt gtcgcttatc tcaatttctc accctatctg
    25261 caaacggctg gcactctgga tgctaaaacc gtggcgatta tttccttcgc gttgtgcggt
    25321 ttcgctaact ttggttctat cggggtggtg gtgggggcgt tttctgcggt tgcgccacac
    25381 cgtgcgccgg aaatcgccca gcttggttta cgcgcgctgg cggcggcgac actttctaac
    25441 ctgatgagtg ccaccattgc cgggttcttt attggtttag cgtagtgtgc gatttatgag
    25501 ccggataata tgcggtcaag cgtcgaatcc ggcaacattg ttacaccatt ggcattaatg
    25561 aaagcgcgtt atcggcagac aaggtagggt tatttaagct gccgctggcg cgtgaaattg
    25621 ccgcgcaggc catcgcaaaa cgggcgctgt cacggaaatt gtttccttcc agaaagctat
    25681 ataccaggcc cgccataaaa ccatcgtcag caccaaaact gtctactgtc gtatgcgctg
    25741 gtgcggtcag caaaaattgt tctccatcct tttcgctgca ataaactgac tcatcgggca
    25801 aataaacaaa cagttgctga acaccttgct gatgcaacgc attcactgca gcattacggt
    25861 cagcatcgct ggtgatcgcc tgtccccata aaatttccag ctccggtaaa gtgggtttca
    25921 gggtgtgaat atgcgccagc cagtgtttga ttttgcccgc tttgaattct gaaacggtat
    25981 cgacaaacac cgggatctca ccagcgaggg taaagaccca ttccagcgcc tcggctgtca
    26041 ggttgcaatc agccagtacc acgcccgcat gacgaagtaa atcgcgcgac ccgttcaata
    26101 attgcggcga taactgttcc agcagatggg tatcgttaat cgccagcacg gtttcatcgt
    26161 ctcgattggc aattgccaga tacgtcgatg tgctttgacc atgcaaacga acgcagccgg
    26221 agacattcac gccggcacgg cgcgtttctt ccaggagcat ttcgccataa aagtcatcgc
    26281 caatcactga aagcaaatgg acgtcgcggc ctaacagcgc cagattgtgg gcgatgttgc
    26341 gtcccacgcc gcccgctgag caatgaattg taccgggatg agaagccgat tgcgggtaac
    26401 ggatatccgc catcccgcga atatccatat tgattgtccc caccactacg cagtattcct
    26461 gctcggtgag aatgtaacct ttgcctttaa tcaggccttt gcgcattaaa tccataatat
    26521 gcgccgcaac gcgcgaacgg ctgatttgca gcatgtccgc aatttcgttc tgctgaatca
    26581 gcgggttacg ccgtaaaatt gcaaggatct ccttttcccg gttattcatc ttcaggctac
    26641 cgctttttca gtttgttgcg ctttcagcca ggcgatctct tctgcccaga tatccgggtt
    26701 gatggtttcg aggatcagcg gaatgccgtc gaaacggtcg tcctgcatga tccagcggaa
    26761 cgcatcatga ccgatattgc cttcaccgag gctatgatgg cggtcaacgc ggctgccaaa
    26821 ggtgcttttc gcatcgttaa ggtgcatccc gcgcagatac ttaaagccga caatacgggc
    26881 aaaatcagcg aacgttttct cgcattcggc tggagtacgc aaatcatacc cggcagcgaa
    26941 agtatggcag gtatcaatgc agacgccgac acgggattta tcttccacgc cgtcgataat
    27001 cgccgcgaga tgttcgaatt taaaccctaa gttactgccc tgaccggcgg tgttttcaat
    27061 caccgcggtc acaccttgag ttttatccag cgcaatgttg atggattcgg caatacgcgc
    27121 aaggcaatcc tcttctgaaa tctgcatcag atggctgcca gggtggaagt tgagcaaaga
    27181 aagccccagc tgttcgcaac gctgcatttc atctataaag gcatcgcgcg atttttccag
    27241 agcttcagtg accggatgtc cgaggttaat cagataactg tcgtggggaa gaatttgcgc
    27301 cgatgtgtag tgatattttt cacaggcggc tttgaattca tcgatggttt gcgtcgtgag
    27361 cggtgcggca cgccactgac gttggttttt ggtgaacaag gcaaacgcgg ttgcgtcgat
    27421 ttcggcggcg cgaattgcgg catttgccag accgccagca gcactaacgt gcgctccaat
    27481 gtatttcatg cgaggactcc tgttaaaccc gctggaggaa aacggtaatg atagcgggtt
    27541 aacaggagca agatgtagtg gtttatgcga tgacgctttg aatcacatag ttaatcgcac
    27601 caccaccaac aatcagccag gcaaacagta ccagtgccat cagcagaggt ttcgccccag
    27661 cttttttcag tgcgctgacg tgagtggtca gacccagcgc cgccatcgcc attgccagca
    27721 ggaaggtatc cagcgttacc agcatgttca ccacgctctg cggtaacagg tggaacgagt
    27781 taaagatggc aactacgatg aacaagatgg caaaccacgg aatagtgatt ttgcttttct
    27841 cgccgctgtt cgccccagaa agctgtttaa cacgcgccgc cagcaggatg aggaacggag
    27901 ccagcatcat cacgcgcagc attttggaaa taactgctgc gttttccgca tccgggctga
    27961 tggcatgacc tgccgccacc acctgcgcca cttcgtgcac agtagaacca atgtagatac
    28021 cgaaagtttc cggactaaac cattgagaca tcagcggata tatcgccggg tagaggaaaa
    28081 tcgcgacggt cccgaagata acaacggttg caacagccac ggttacttta ctggcttccg
    28141 ctttcactac cggctcagtc gccagtaccg cggcagcacc acagatactg ctaccggcac
    28201 cgatcaacca gctggtgtgc ttatccagac caaacacttt ctgccccagg aagcaagcca
    28261 gcaggaaggt actggacagc gtcaacacgt caatgatgat cccactgata ccgacatcgg
    28321 caatttgcga gaacgtcaga cggaagccat aaagaatgat acccagacgt aataaatatt
    28381 gcttggcaaa cagcacacca ccgtcacagc ttttccagat gtgcggatag atggtgttgc
    28441 ctaaaaccat ccccaacaag attgcgaggg tgagggcact aaacccggca cccgcaaccg
    28501 cgggaatgga accaccccac agggcgaccc cggtgataac tgcactcagg gctaaccccg
    28561 gaataaaatg ccacagtgta cgatgttgtt tctgtaaggt gatattcgtc ataaccctct
    28621 cctttaaccg ggcataaggt tacgactaac tggtttaaaa ataaaattga ttatatattt
    28681 ataattaatc tttataagtg gtaagcgact atgcacatca ccctccggca gttggaagtt
    28741 tttgcagaag tattgaaaag tggatcaacc acccaggcgt cggtgatgct ggcgttgtcg
    28801 caatcagcag tgagcgcagc cttgaccgac ctggaagggc aacttggcgt gcaactgttt
    28861 gatcgcgtgg ggaaaagact ggttgttaat gaacacgggc ggctgctcta tccgcgtgcg
    28921 ttggcattgc ttgaacaggc ggttgaaatc gaacaactgt ttcgcgaaga caacggcgcg
    28981 attcgtatct atgccagtag taccatcggt aactacattc tgcctgcagt tatcgcccgt
    29041 tatcgccatg attatccgca gttgccgatt gaacttagcg ttgggaacag ccaggacgtg
    29101 atgcaagcgg tgctggattt ccgcgttgat attggcttta ttgaaggacc gtgccacagc
    29161 actgaaatca tttctgaacc gtggctggaa gacgagctgg tggttttcgc cgcgccgact
    29221 tcgccgttgg cccgtggtcc ggtcacctta gaacagctgg ccgctgcgcc gtggatcctg
    29281 cgtgaacgcg gttccggcac gcgggagatt gtcgattatc tgttgctgtc acatttaccg
    29341 aagtttgaga tggcgatgga attaggtaac tccgaggcaa tcaaacatgc ggtgcgtcat
    29401 gggttgggaa ttagttgcct gtcgcgacgg gtgattgaag accaattgca ggcaggcaca
    29461 ttaagtgaag ttgcggtccc tctgccgcgc ctgatgcgta cgttgtggcg tatacatcat
    29521 cggcaaaaac acctttccaa cgcgctacgg cgctttctgg actattgcga tcccgcgaat
    29581 gtaccgtgtt aagttgctgt acaagaacat gctggtgctg tgtcgatttc gtgacgcagc
    29641 gccctcagca tgcattcgcc agaaaagaga ttggctgctt tacttataat ccctgggcga
    29701 tcatgaaggt gtcttataac cgtgtatttc tgccggaagg attgccaatc gtctgctaca
    29761 atcgcgcctc atttttaaga tggatagcat ttttgtatgg tttccgaaac taaaaccaca
    29821 gaagcgccgg gcttacgccg tgaattaaag gcgcgtcacc tgacgatgat tgccattggc
    29881 ggttccatcg gtacaggtct ttttgttgcc tctggcgcaa cgatttctca ggcaggtccg
    29941 ggcggggcat tgctctcgta tatgctgatt ggcctgatgg tttacttcct gatgaccagt
    30001 ctcggtgaac tggctgcata tatgccggtt tccggttcgt ttgccactta cggtcagaac
    30061 tatgttgaag aaggctttgg cttcgcgctg ggctggaact actggtacaa ctgggcggtg
    30121 actattgccg ttgacctggt tgcagctcag ctggtcatga gctggtggtt cccggataca
    30181 ccgggctgga tctggagtgc gttgttcctc ggcgttatct tcctgctgaa ctacatctct
    30241 gttcgcggct ttggtgaagc ggaatactgg ttctcactga tcaaagtcac gacagttatt
    30301 gtctttatca tcgttggcgt gctgatgatt atcggtatct tcaaaggcgc gcagcctgcg
    30361 ggctggagca actggacaat cggcgaagcg ccgtttgctg gtggttttgc ggcgatgatc
    30421 ggcgtagcta tgattgtcgg cttctctttc cagggaaccg agctgatcgg tattgctgca
    30481 ggcgagtccg aagatccggg gaaaaacatt ccacgcgcgg tacgtcaggt gttctggcga
    30541 atcctgttgt tctatgtgtt cgcgatcctg attatcagcc tgattattcc gtacaccgat
    30601 ccgagcctgc tgcgtaacga tgttaaagac atcagcgtta gtccgttcac cctggtgttc
    30661 cagcacgcgg gtctgctctc tgcggcggcg gtgatgaacg cagttattct gacggcggtg
    30721 ctgtcagccg gtaactccgg tatgtatgcg tctactcgta tgctgtacac cctggcgtgt
    30781 gacggtaaag cgccgcgcat tttcgctaaa ctgtcgcgtg gtggcgtgcc gcgtaatgcg
    30841 ctgtatgcga cgacggtgat tgccggtctg tgcttcctga cctccatgtt tggcaaccag
    30901 acggtatacc tgtggctgct gaacacctcc gggatgaccg gttttatcgc ctggctgggg
    30961 attgccatta gccactaccg cttccgtcgc ggttacgtat tgcagggaca cgacattaac
    31021 gatctgccgt accgttcagg tttcttccca ctgggtccga tcttcgcatt cattctgtgt
    31081 ctgattatca ctttgggcca gaactacgaa gcattcctga aagataccat tgactggggc
    31141 ggcgtagcgg caacgtatat tggtatcccg ctgttcctga ttatttggtt cggctacaag
    31201 ctgattaaag gaacccactt cgtacgctac agcgaaatga agttcccgca gaacgataag
    31261 aaataagttt ctcccttcct tgctaagccc tctcaaccga gagggctttt tcaattccat
    31321 ttccctgaca aatcatgcgg atataaaatt taacatttgg attgataatt gttatcgttt
    31381 gcattatcgt tacgccgcaa tcaaaaaagg ctgacaaatc agaggctggt ccggcttgct
    31441 gggatgatca cctgcataca aataagtcca ccgcgatgct gccgtacgca aggggacgtg
    31501 aagaagatgt gagcgataac ccattttatt ttcgtagtta cctcatggag atatggaatg
    31561 tttaggttga accctttcgt acgggtcggg ctgtgtttgt ccgctatttc ttgtgcatgg
    31621 cctgtgttag cggtcgatga tgatggcgaa acgatggttg tcactgcatc ttccgttgaa
    31681 caaaacctta aagatgcacc cgccagtatc agcgtcatta cccaggaaga cctgcagcga
    31741 aaaccggtac agaatctgaa ggatgtcctc aaagaagtgc ctggcgtaca actgacgaac
    31801 gaaggggata accgtaaggg cgttagtatt cgtggtctgg acagcagcta caccctgatt
    31861 cttgtcgacg gtaaacgcgt taactcccgc aatgccgtct tccgccacaa tgatttcgat
    31921 ctgaactgga tcccggtcga ttccatcgaa cgtattgaag tggtccgtgg cccgatgtcg
    31981 tcgctgtacg gttccgatgc gctcggcggt gtagtgaata tcatcaccaa aaaaatcggt
    32041 cagaaatggt cgggtaccgt taccgtcgat accaccattc aggaacatcg cgatcgcggt
    32101 gatacctata acggtcagtt ctttaccagt ggaccattaa ttgatggtgt gctgggaatg
    32161 aaagcttacg gcagcctggc aaaacgtgaa aaggatgacc cgcaaaactc aacgaccacc
    32221 gataccggag aaacgccgcg tattgaagga ttctccagcc gcgacggcaa tgtcgaattt
    32281 gcctggacac cgaatcaaaa tcacgatttt actgccggat acggtttcga ccgtcaggat
    32341 cgtgattccg actcgctgga caaaaaccgc ctggaacgcc agaactactc cgtcagccat
    32401 aatgggcgtt gggattacgg caccagcgaa ctgaaatact acggtgagaa agtcgagaac
    32461 aaaaaccctg gcaacagcag cccgataact tccgaaagca atacggtcga cggcaaatac
    32521 acgttgccgc tgacggcgat taatcagttt ctcacggttg gcggtgaatg gcgtcacgac
    32581 aaacttagcg atgcggtgaa cctgaccggg ggaaccagct ccaaaacgtc tgccagccag
    32641 tacgcgctgt ttgtggaaga tgaatggcga atcttcgagc cgctggcgct gacgaccggc
    32701 gtacgtatgg acgatcatga aacctacggt gaacactgga gtccgcgtgc ctacctggtt
    32761 tataacgcca ccgacaccgt aacggtgaaa gggggctggg cgacggcatt taaagcgcct
    32821 tctctgttgc aacttagccc tgactggacg agcaattcct gccgtggcgc atgtaagatt
    32881 gtgggtagcc cggatctgaa accagaaacc agcgaaagtt gggagctggg gctgtactac
    32941 atgggtgaag aaggctggct ggaaggggtt gaatccagcg ttaccgtttt ccgtaacgat
    33001 gtgaaagatc gtatcagcat cagccgtacg tctgacgtca atgctgcacc gggctaccaa
    33061 aactttgttg gttttacagc gaacggtgtg ccggtattta gctactacaa cgttaacaaa
    33121 gcacgtattc agggcgtgga aaccgaactg aaaattccgt tcaacgatga atggaaactg
    33181 tcactcaact acacctacaa cgatggtcgt gatgtcagca acggcgaaaa caaaccgcta
    33241 tccgatctgc cgttccatac cgctaacggt acgctggact ggaaaccgct ggcactggaa
    33301 gactggtcat tctatgtttc tggtcactat accgggcaga aacgagccga cagcgcgacg
    33361 gctaaaacac cgggcggtta taccatctgg aataccggcg cggcctggca ggtgactaaa
    33421 gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag tcgtgatgac
    33481 tacagctata acgaagacgg acgtcgttac tttatggcag tggattatcg cttctgatga
    33541 gaagatgccc ggcgaaccgg gcggactttc acttcagtaa atactgcgca tggaagcgca
    33601 ggtgatcctc tataaaagag gcgatgaagt agtaactgtg atcatatccc ggctgaatac
    33661 gcagcgtcat cggccacgct ttctggcgcg cggcttctgc cagaactgcg ggttgcaact
    33721 gatcggcaag aaactgatca ttatcgccct gatcgataag cgtcgggatc gcatcctgcg
    33781 cgttactggc atacatcagc gcgcaactgt cccattccag ccatgcattt ttgtcctcac
    33841 ctaaatagcg gctaaacgct ttgattcccc acgggacgct gcacggattc acaattggcg
    33901 caaaggccga aacgctggtg tatttgcccg ggtttttcag cgccataatc agcgcaccgt
    33961 gaccacccat tgaatgaccg ctaatagcgc agcggtcgct gacattaaat tgcgactgaa
    34021 ccagcgccgg taattcatcg cgcagataat catacatccg gtaatgcgtc gcccacggcg
    34081 gttgcgtggc attaagataa aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg
    34141 caaccttttc gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc agttctgccg
    34201 ccacgcgctg ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa
    34261 gccagtacag cactggtggc ggggtgtgat cacgaggggg tgggagaaag atactgaacg
    34321 tcatcgggca gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac
    34381 agcggtgctc ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc
    34441 atcataccgt ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg
    34501 ttttaacatc tgctgatgaa aggcagcggc aattacaata attatcgctg tgaatactgg
    34561 attatgtgcg ccgcctcacg cacaataatc aggctgtaaa tcagcttaat aactttgccc
    34621 ccacgcaggg cggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa
    34681 gaagcggccc tggttcatga agcgttagtt gcgcgaggac tggaaacacc gctgcgcccg
    34741 cccgtgcatg aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa
    34801 atcatgcagc tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc
    34861 atcgctaaaa tgtatgtcga tgaaattttc tccggtctgg attacgccaa cttcccgaaa
    34921 atcaccctca ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact
    34981 ctgaccagta cctgtgaaca ccattttgtt accattgatg gcaaagcgac ggtggcttat
    35041 atcccgaaag attcggtgat cggtctgtca aaaattaacc gtattgtgca gttctttgcc
    35101 cagcgtccgc aggtgcagga acgtctgacg cagcaaattc tcattgcgct acaaacgctg
    35161 ctgggcacca ataacgtggc tgtctctatc gacgcggtgc attactgcgt gaaggcgcgt
    35221 ggcatccgcg atgcaaccag tgccacgaca acgacctctc ttggtggatt gttcaaatcc
    35281 agtcagaata cgcgccacga gtttctgcgc gctgtgcgtc atcacaactg attaaaaggc
    35341 aggaaccatg gagcgcaacg tcacgctcga ttttgttcgc ggcgtcgcca ttctggggat
    35401 cctgctatta aacatcagcg cctttgggct accaaaggcg gcttatctta atcccgcctg
    35461 gtacggcgct attacgccgc aggatgcatg gacttgggca tttctcgatc tcatcggcca
    35521 ggtgaaattc ctcacgcttt ttgcgctgct gtttggtgct ggcctgcaaa tgttgctgcc
    35581 ccgtggcaga cgctggatcc agtcgcggtt aacgctgtta gtcttgctgg gctttattca
    35641 cggtttattg ttctgggacg gcgatattct gctggcttac gggctggtgg gcttaatctg
    35701 ctggcggctg gtgcgcgatg cgccatcggt aaaaagcctg tttaataccg gcgtcatgct
    35761 ttatctggtg gggcttggcg ttttgctgtt attggggttg atttccgata gccagaccag
    35821 ccgcgcctgg acgccggatg catcggctat tttgtatgaa aaatactgga agcttcacgg
    35881 cggcgttgaa gcgatcagta atcgtgccga tggtgttggc aacagtttac tggcactggg
    35941 cgcacagtat ggctggcaac tggctgggat gatgctcatt ggtgccgcat tgatgcgcag
    36001 tggctggctg aaagggcagt tcagcttacg tcactatcgt cgtactggtt ttgtgctggt
    36061 ggcgattggg gtgatcatta accttcctgc catcgccctg caatggcggc tggactgggc
    36121 atatcgctgg tgcgccttct tacttcagat gccgcgggaa ctgagtgcac cgtttcaggc
    36181 gattggctat gcgtcgctgt tttatggttt ctggccgcaa ttgagccgct ttaagctggt
    36241 gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac tatctattgc aaacgctgat
    36301 ttgtaccacg cttttttatc acctcggttt gtttatgcag tttgaccgtc tggaattgct
    36361 ggcgtttgtt attccggtat ggctggcgaa tattctcttc tctgttatct ggctgcgtta
    36421 cttccgccag gggccggtgg aatggctctg gcgtcagtta actttgcgtg ctgccggacc
    36481 agcaatatct aaaacatcaa gataacgatc tggatcacat tcgttaacaa aacggctgta
    36541 accgtttcca ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg acaaaatagc
    36601 cgccagcaag cagtcattta ctgcaatctc ataacaggta gtgaatatga tcaccattcg
    36661 tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt tcccgggtgc tcaataacag
    36721 cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg agctggatta
    36781 tcggccaaac gccaatgccc aggcgctggc aactcaggtt agcgacacca ttggcgtggt
    36841 ggtgatggac gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg atctggtcgc
    36901 ccagcagcat cagaaatacg tgctaatcgg caatagctat catgaagcgg aaaaagagcg
    36961 tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgca ttgattgttc actcaaaagc
    37021 attgagtgac gatgaactgg cgcaatttat ggataacatt cccggtatgg tgttaatcaa
    37081 ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg gataatctca gcggtgcccg
    37141 aatggcaacg cgcatgttgc tgaataacgg acatcaacgt attggttatc tttcttccag
    37201 tcacggcatt gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca
    37261 ggatattatt ccgccggaaa gctggattgg cactggcacg ccggacatgc cgggtggaga
    37321 ggcggcgatg gttgaactgc tggggcgcaa tctacaactt accgctgtat ttgcttataa
    37381 cgacaatatg gccgctggcg cactgacagc attaaaagat aatggcattg cgattccgtt
    37441 acatctctca atcatcggtt tcgatgatat tcccatcgcc cgttacaccg acccgcaatt
    37501 aacgaccgtg cgttatccca ttgcttcaat ggctaaatta gccaccgaac tggccttgca
    37561 gggtgcagca ggcaatattg atcctcgtgc cagccactgt tttatgccga cgttagtgcg
    37621 tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc actaactcaa caaatcaggc
    37681 gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc ttaacaatgt gatagctatg
    37741 attgcaccgt tttaacgttg taacccgtat gtaacagtga ataatcactt tcaccgaggt
    37801 aatagcgtca taacaacaat taaagccgtt ttctggagcg ttaccgggca tggaagaacg
    37861 aattttaaaa agtgagcttc ggcgttcagt aacacttcat taactctact gccccgccga
    37921 gcatttatct caagcactac cctgcataag aaaaaccgga gataccatga ataagaaggt
    37981 gttaaccctg tctgctgtga tggccagcat gttattcggt gccgctgcac acgctgctga
    38041 tactcgcatt ggtgtaacaa tctataagta cgacgataac tttatgtctg tggtgcgcaa
    38101 ggctattgag caggatgcga aagccgcgcc agatgttcag ctgctgatga atgattctca
    38161 gaatgaccag tccaagcaga acgatcagat cgacgtattg ctggcgaaag gggtgaaggc
    38221 actggcaatc aacctggttg acccggcagc tgcgggtacg gtgattgaga aagcgcgtgg
    38281 gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc tggatagcta
    38341 cgacaaagcc tactacgttg gcactgactc caaagagtcc ggcattattc agggcgattt
    38401 gattgctaaa cactgggcgg cgaatcaggg ttgggatctg aacaaagacg gtcagattca
    38461 gttcgtactg ctgaaaggtg aaccgggcca tccggatgca gaagcacgta ccacttacgt
    38521 gattaaagaa ttgaacgata aaggcatcaa aactgaacag ttacagttag ataccgcaat
    38581 gtgggacacc gctcaggcga aagataagat ggacgcctgg ctgtctggcc cgaacgccaa
    38641 caaaatcgaa gtggttatcg ccaacaacga tgcgatggca atgggcgcgg ttgaagcgct
    38701 gaaagcacac aacaagtcca gcattccggt gtttggcgtc gatgcgctgc cagaagcgct
    38761 ggcgctggtg aaatccggtg cactggcggg caccgtactg aacgatgcta acaaccaggc
    38821 gaaagcgacc tttgatctgg cgaaaaacct ggccgatggt aaaggtgcgg ctgatggcac
    38881 caactggaaa atcgacaaca aagtggtccg cgtaccttat gttggcgtag ataaagacaa
    38941 cctggctgaa ttcagcaaga aataagactg attgggcgca gtctattact gcgcccttat
    39001 tgtatgacca acaaggtaaa attatatggt cagctcaacg actccgtcct ccggggaata
    39061 cttgttggaa atgagcggta tcaacaagtc ttttcctggt gttaaggcac ttgataacgt
    39121 taatttaaaa gtccggccac attctatcca tgcattaatg ggggaaaacg gtgcaggaaa
    39181 atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca ccattttatt
    39241 ccagggtaaa gagatcgatt tccattcggc caaagaagca ctggaaaatg gtatttcgat
    39301 ggtacaccag gagttaaacc tggtattaca acgttcggtg atggacaaca tgtggcttgg
    39361 gcgatacccc accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg aaaccaaagc
    39421 gatttttgat gaactggata ttgatatcga tccgcgtgcg cgcgtcggca cattatctgt
    39481 ttcgcaaatg cagatgatcg aaatcgccaa agcgttttcc tataacgcga aaattgtgat
    39541 tatggatgaa ccgacttctt cgttaaccga aaaagaggtc aatcatctgt tcactattat
    39601 tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa tggaagaaat
    39661 cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg ccaccgaacc
    39721 gctggcagga ctgacgatgg ataagatcat cgccatgatg gttgggcgtt ctcttaacca
    39781 gcgtttccct gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc gtaacctgac
    39841 gtcactgcgc cagccgtcga tccgtgatgt ctcgtttgat ctgcataaag gggagatcct
    39901 cggtattgcc ggtctggtgg gggcgaaacg taccgatatt gttgagacgt tatttggtat
    39961 tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa aagatcaata accataatgc
    40021 caacgaagcc ataaaccacg gatttgcact ggtaactgag gagcgccgct caacaggaat
    40081 ttatgcctat ctggatattg gttttaactc gttaatttcc aatattcgca actacaaaaa
    40141 taaagttggt ttactggata actcgcggat gaaaagcgat acccagtggg tgattgattc
    40201 gatgcgggta aaaacaccgg ggcatcggac gcaaattggt tcgctctcag gtggtaatca
    40261 gcaaaaggtg attattggtc gctggctact aacgcaacca gaaatattaa tgctcgatga
    40321 accgacgcgc ggtattgatg tcggggcgaa gtttgagatc tatcagttaa ttgccgaact
    40381 ggcgaagaaa ggcaagggga ttattattat ctcctctgaa atgcctgagt tgttagggat
    40441 aacagaccgt attctggtca tgagcaatgg tctcgtttcc ggaattgtcg atacaaaaac
    40501 aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt aggggcttcc
    40561 catgagtgcg ttaaataaga aaagttttct tacttacctg aaagagggcg gtatttacgt
    40621 cgttctttta gttttgctgg cgattattat tttccaggac ccaacatttt taagtctgtt
    40681 gaacttaagt aatattctca cccagtcatc ggtgcgtatt attatcgcgc tcggtgtggc
    40741 agggttaatt gtcacccagg ggaccgacct ttctgcgggg cgtcaagtag ggctggcggc
    40801 agtggtggca gcgacattat tgcagtccat ggataacgcc aacaaagtgt tcccggaaat
    40861 ggcgacgatg ccgattgcgc tggttattct gattgtctgt gccattggtg cggtgatcgg
    40921 tttgatcaac ggtctgatta tcgcttatct caacgttacg ccgttcatta ccacgcttgg
    40981 cacgatgatc atcgtctatg gcatcaactc gctctattac gatttcgtcg gggcgtcgcc
    41041 aatttctggt tttgacagtg gcttctctac ctttgctcag ggctttgtcg cgctggggag
    41101 tttccgtctc tcttacatca ccttctacgc gttgattgcg gtggcgttcg tctgggtgtt
    41161 gtggaacaaa acccgcttcg gtaagaacat ttttgccatt ggcggtaacc cggaagcggc
    41221 aaaagtatct ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt tgtctggcgt
    41281 gttctatgcc tttggcggga tgttagaagc cggacgtatc ggctctgcca ccaacaacct
    41341 cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggcg tatcgttcag
    41401 cggcggtgtg gggacggtga ttggcgtggt gaccggggta attattttta ccgtcatcaa
    41461 ctatggcctg acgtacatcg gtgtaaaccc gtactggcag tacatcatca aaggggcgat
    41521 tattatcttc gccgtggcac tggattcact gaagtacgcg cgtaagaagt gatatgattg
    41581 tggttcgcat tcggtcataa acgcacataa catcatgatc tgtgtggttt tgtaggccgg
    41641 ataaggcgtt cacgccgcat ccggcgacag gagccaaatg ccggatgcaa cgctggcgcg
    41701 ttttatccgg cctacaatga gctcaacata ctgatttgta tggttttgta ggccggataa
    41761 ggcgttcaag ccgcatccgg cgacaggaac caaatgccgg atgcgtctct ggttttacaa
    41821 cgttaccggg tgttctttct cgcctttctt aaacttcact tccccgagct caatacaacc
    41881 caccgggcag acgtgaccac aaagcagaca acccacacat ttctcggtat tacaatgcgg
    41941 tgtgcgggtt ttctcgctcc attccatcgc ctggtgaccg ccgtcgtaac aggaaatata
    42001 acagcgtcca cagccaacac atttatcaag attgatacgg ggatagacaa tataactgcg
    42061 gtctaaatct tccgccggga caatattgtt attcgccaga cctaccatct cctgcaacga
    42121 atcaaatccc tgatcggcga gataatgcga caacccgctc gccatatctt ctacgatgcg
    42181 atagccgtac tgcataatgc cggtggtcac ctgtaacgtt gctgcgccga gcaataaaaa
    42241 ctccgccgca tcctcccagg tttcaatgcc gccgataccg ctgattggga aatcgcgcag
    42301 ttctggatgg gtgcgcattt gctgaatgaa gcgcagggcg atcggtttta ccgctttacc
    42361 ggaatatccg gaaatactcg attttccgtt aacgatcggc ataccgattt tctgattaag
    42421 atcgatattg gtgatggatt taacggtgtt aatcgccgca atgccatctg cgccgccgcg
    42481 cttcgccgcc agcgccactt cgcacatatc accgatattc ggcgtcatct tcgccagtat
    42541 tggcagcgtg gaaccccgtt tcactgcccg acaatatttt tctaccagct ccgggctttg
    42601 cccgacatcg ctacccatcg catgagaagt catttgcgga caggagaagt tacactcgat
    42661 catatccgcg ccagcttctt gcaccaggcg cgccagctcc tcccattgct gctcattttc
    42721 ccccatgatc gaagcgatca atactttgtc cgggtaatct tccttcagcc gacgcagggc
    42781 ggccagattc tcttccaacg gatgttcagc aatctgctcc atatttttga agccgataaa
    42841 accggtatct tctttcacca gatgatcaaa acgcggcgag acttcgttgg cgataaaaaa
    42901 gccgatcgtt ttaaacacca cgccgcccca acctgtgtcg taggctttgg cacacatctc
    42961 atagcagttg cctaccggcg aagaagagag gcagaacggg ttgggaaact tcacgccgca
    43021 aaaagtaatc gaaagatctt tcgttaacat gagcaagctc cctctaaata gtgatgaatc
    43081 gcctcggcgg cttctttccc ggttttcacg gcatagacca cggttttgtc accttcaaca
    43141 atatcgccag cagcaaagac ttgcggatcg cgggtctgat aattttgtgt tttgatggtg
    43201 ttacgctgcg gctctaactc cgcaaaggca tccagtctgg catgctgacc gacggcgaga
    43261 ataattttat ctgccaccat cgtcagttcg cccgataacc gtacatgctt aaacgtgact
    43321 ttattgcctt ccacggctac tggcgtgaat ccatcgatga tcgaaacgcc cagctcccgg
    43381 gcgctggcaa actctttttc gcttgccgga aactcatcta actcttcacg agctacgcaa
    43441 gttaccgcct gacagccgag aactttcagc gtgctggcaa cgtccatcgc gacatcaccg
    43501 ccgccgataa ttaatgcgct ttgtggaatg ctgatatcgc cttgcgcctg gcgtgcacat
    43561 tgcaagaagt caacggcaat ctcaacgtcg ctatgctcaa acagcggtag cccggaaccg
    43621 ctcgataacc ccacggtgac cagtaccgcg cggttttccg ctttcagttg ctccagggtt
    43681 agtgttttac cgacttcgtt gttgcactta atggtcacgc ccattttttc aatacgggcg
    43741 atctctgcat ccagtactga ttgcggtaac cggaattgcg gaatgccgtt acgcaaccag
    43801 ccaccgggtt gtgcttcttt ctcatagatc gtgacgtcat aaccctggtt tgtcagtgtc
    43861 acactggcct gcaatccggc aggacctgcg ccaataatcg cgactttgcc gagcgtttta
    43921 gtaccgggct gataaatttc catcccggtt tgttgttcaa aatcagtaac aaaacgctgt
    43981 aagcggccga tatcaatggg agcatcaaca ccggcacggg tacaaccgct ttggcataat
    44041 ttctccgtcg ggcacactct ggcacaaacg gcaccgaggg cattattttc gcgaattgtc
    44101 tcggcagcgc ctttaaaatt acgaaagtag attgagcgaa taaatttccc cggatcggtc
    44161 tgtgccgggc aagcctgact acagggagcg tcgtgacata ataaacagcg agaggcttct
    44221 ttaatcgcca gtaacggcgt aaaagccgga gtgagttcat ccagataatt ttgttgcggc
    44281 atgtgatctt ccttaatgct atgagtaatt aaggcgaacg ctaaataaat attgcgtgct
    44341 gatattaagg ctatgcgcgt ggggaaattt aaactgtgaa aggattcaca aatagctgcg
    44401 gttcagaatg ttatatccgc agcttattga gtgaagtaat aattattctc aataattaac
    44461 agaaaagaga aaatattaac gatagaaaat aagataaaca atcatgccga tagataaaat
    44521 tccgaagccg atatatgcca ccatataagc gaaaaagagt ccgggatttt ttgctcgcca
    44581 gacatacacc ggttccggtg catttttaac ccgtttatca atcgcgcctg cgcgccagcc
    44641 tttccatgct tcgcggaaac aaaatatcgg gataaggaaa aaaacggcaa cgacaaaaaa
    44701 ctgctgtaca tccataacta ctttccttgt ttcttttgta attcaagtaa ctgttcgggt
    44761 gtgacggcgg gatacccctg cgcatcttcc ggtacctggt gcatagccgg aatagggacc
    44821 agcggcccta aaaaacgcgg ttcacgttta aaaatataaa ggtcagccag cgcaccgaaa
    44881 cgggcggcaa attcacgaat acgtactgac agcatatctt tcggtgacgg tacggcataa
    44941 cactgagcct gaatccccat atgcagcgca ataaataatg cccgctcaca gtggaaacgt
    45001 tgggtgataa taatgaaatc attagtatcg aaaactttgc gtgtacgcac gatggaatcc
    45061 agcgtacgaa agcctgcgta atcgagaaca atatctgatg ggtcgacacc agcagcgatt
    45121 aaatctttgc gcatggtcat cggctcatta taactttgca atgcgttatc gccgctcagt
    45181 aatagataat ttaccttacc gctgttatag gcattaatcg ctccttgaat gcggtagcga
    45241 taatactgat taattacgcc agtacgataa tattttgctg ttccgagcac cacaccgacc
    45301 tggcggtagg ggagatcctg caattcgtcg tagatataag gcgcggtttt ccagctcatc
    45361 cagcgatcga ggccgagcac agtcaacagc agcaagccga taaggactaa caggctgagg
    45421 aacacgcgct ttaacatgca ggagactcga tctaaggaaa atttccatca ggctacttta
    45481 cccgctggtt ttgcgcaaga aacggcaaac tgtttgccgg gattttcgac accaggagag
    45541 ggctggcaag ccggacaagg cgttcacgcc gcatccggca ccaggcttaa gcgagaagca
    45601 cgcggtcgat actgtgacag ccgagcgctt tcagcgttgc ggaggtggca tcccactgaa
    45661 tcaacggcgc atcggctttt tctgccagaa ccgcgcgctg gatatccggg taatcataac
    45721 ccttgagatt caacagaatt aacgctccct gcaacggcgg cagagtcggg ttgaatgcgg
    45781 cgttttcagc gtagctgccg ctgaaaattc gaccgtcttt acattccagc gcaacaccgc
    45841 ttggcgactt actgtaaggc atgtgcgaac ggtttgccgc cgcaatcgcc gcctgagaaa
    45901 gcgcatcacc tgtcagcgca tacccgtgat cctgctcgtc catcagcagc gttttaatct
    45961 ccagatcttt cggcccaaag gcatccggca gatagtcacg cagcgcgtgt gcctcgcggc
    46021 ccggcagatg aatacgcaga tccagaccgc tgttcagttc attcataaac tgacggcagt
    46081 gaccacaagg cgtgtagtta acggtgatgg ctgcaagcgc tttttcacca ctcaaccagg
    46141 cgtggctgat cgcgctttgt tcggcatgaa cggtttgctg cattgtcgca ccaataaatt
    46201 ccatattggc accaaaatac caggttccgc tcacaccgcg cgcaattgcg ccaacattaa
    46261 aattcgacaa tggcgtacgc gcacaggccg ccgccagcgg aagtagtgcg aatgccagcg
    46321 cgtcttcgtc cagccccgtt gcgctcttca gcgatgagac ttgctccccg gtcaacaaag
    46381 cggggaagta cttgtctgcc agaataggtt ccagtgcaga ttgcaagtta tccgcaagtt
    46441 gggcaaaagc ggtttgaaaa cgtggatgca tggcgttgcc tcataatcat gtaatgggat
    46501 aacagtttac gaacccgtta tggctttata tgtgatctga atctcattaa ttatgcaacg
    46561 cgtgaaactt acatcaaaaa tgcgcgacgc atcgcaaatt ttagcccatt actgccagaa
    46621 taatcgggaa aaggaacggt gcgatcagcg aagtaattat cccgcataac accagcgcta
    46681 gcgaactaaa tgcaccttcc tgataatcca gctcggcgca gcgcgccgta ccgagggcgt
    46741 gcgaggcagt ccccattgcc agaccgcgcg cagctttggt acgaatacgc atcgcattaa
    46801 gcaatgtatg gccaaatacc gcgccgagga tgccgacgaa aatcacgcaa actgcgctga
    46861 ttgccggaat accgccaata ctgccgccaa ccgccattgc aataggcgtg gtgactgatt
    46921 tcggcaggat tgacgcggcg atttccggtg aagcgcccat caacaatgcc acggaagtcc
    46981 ccgtcaccat cgcaaccacg ctgccgataa aacagatggt gatgatcgat ttccagcgcg
    47041 cgcggatctg gtgtagctgc tcatataaag gataggccag cgcgacgacc gccggttgca
    47101 gcagatcgtt tagcacttcg ctacctttaa agtaactgtc gtaagagatg ccggtcagca
    47161 ttaaaaaagg aatgatcacc accattgcta ccagcaacgg gttaagcagg ggaaacttat
    47221 accgtgccgc cagtttgcgg gcggcaaaaa agacaatcaa cgtcagcggt aatgaccacc
    47281 agatattcgc catcattctt ctgatccttt ctgacctacg actttacgtt caccgtgtac
    47341 cagttgcgaa ctccagctca ccaccagaaa aaccaccagc gtactgattg cacaggaaac
    47401 cactaccggg ccaaactgtg cgcggagcaa atcaaaatat tgcatgacgc ctacgccaat
    47461 cggcacaaac aatagcgcca tatagcgaat cagtacgtag caccccggat tgacccattt
    47521 cgccggaaga atttgcaagg ccagcaggac aaacaggatc agcatcccga tgatgctgcc
    47581 cggaatggtt accggtagca gggaagcaat gaaaatgcct gcatacaggc aggcataaat
    47641 caggacgaaa gcgcgtaaat attgccagat aatgttcagt gtcttactca taatcaacac
    47701 cccagatgag atggattcat catacaatta attcgtaaaa cgtgctaccg atcacattaa
    47761 gaattctcag catagttgaa ttttagggag gtaacctcgg gcgagcgccc cgttcagcac
    47821 actaattctg ctgtaaaaag tgcagaaact gacttaaagc ataagaaggc gtttcgttat
    47881 tgcgccagaa cacaccgacg ctacctttct gttctatctt tggcaggttg agtatcacca
    47941 gttgtccctg gctggcataa cgttgcgcca ggcgtaatga caaaatagag atcatatcgc
    48001 tgctttgtaa caggttggta ctgacgttca tcgacgccga ctcaatcgtg ttttctggca
    48061 gcattacgcc gtggtctacc agcgcattat caatactaat gcgaatgggg gtacccgtcg
    48121 gccagacgat ccagcgccag ttggcaagat cattccagct gagggtggcg tatttcgcca
    48181 gtgggtgctc agggcgtgcg acaaagcata ctggctcggt gtacagcacc tgataattca
    48241 gtggcagctg caatgcgcga ccgccaattc gcccaacgac cacatcgaca gaacctgcca
    48301 atagatcttg cagcaatggg gtcatcacct tctcttcgat attcaggtgt agagttggca
    48361 tcttcgtaag caaatgcaaa atcgcctggg atacgcagtc agtggcgacg ggagaacagc
    48421 caattttcag actgccgacc agtccacctt gtttaaaacg ggccagttca tgttgcgatc
    48481 gctccatgtc attgattaac cgttgggcat gttgcaataa caatttgccg ccttcagtcg
    48541 gacgtaatcc tttgctgtgc cgctcaaaaa gtgtgattcc aatctcatct tcgagttgtg
    48601 acaaccattt cgaaagcgca ggttgggtga tattcatcat tcgtgcgaca tgggttagat
    48661 tgccctgttc acccagcgcc accagcattt caaggtgatg tagttttaat ttttgcgccc
    48721 agttcgccat ttgccataac ctccaggtta tctctctgcc cgaaatgcca ttgtacatct
    48781 catctcttta atcacaatat cgcaacatat acaaaacaaa agaaacatct accttacctg
    48841 cactgaggca tttactatga ctcaacgacg tgatttacaa gcacttatcg atgctgctcc
    48901 ggtcggcaag atgcagtggc gcgttatcat ctgctgtttt ctggtggtaa tgctcgatgg
    48961 ttttgacacc gcggctatcg gatttatcgc ccctgatatt cgcactcact ggcaactcac
    49021 cgcaggcgat ctcgccccgc tttttggcgc gggtctgcta ggattaaccg caggcgcact
    49081 gctttgtggc ccgttgtccg atcgctttgg tcgcaagcgg gtgattgagc tgtgcgtctt
    49141 tttgtttggc gcgttaagcc tcgcctcggc tttctcaccg gatctgcaaa cgctggtctt
    49201 cctgcgcttt ctcaccggcc tgggtctggg cggcgcgatg ccgaacacca tcaccatgac
    49261 ctcggagtat ctgcctgccc gccgccgcgg cgcgctggtg acgctgatgt tttgcggctt
    49321 tacgctgggt tcggcgtttg gcgggattgt cagtgcgcag ctggtgccgg tgattggctg
    49381 gcacggcatt ctggtgttgg ggggcgtgtt accgctgatg ctgtttgtcg cactgctggt
    49441 agttctgccg gaatcgccac gctggcaggt gcgccgccag ctaccgcagg cggtgattgc
    49501 gaaaaccgtc agcgccatca cccgcgagcg ttatgtcgat acccactttt atctcattga
    49561 atcagcctcc gttacaaaag gcagcattcg tcaactgttt atgggacgtc agttacccat
    49621 cacgttgatg ctgtgggtag tgttctttat gagcctgctg attatttacc tgctctcaag
    49681 ctggatgccg acgctactga accatcgcgg catcgacctg caacacgcgt cctgggtcac
    49741 cgccgcgttc cagattggtg gtacgttagg cgcgctggcg ctgggtgtgc tgatggataa
    49801 gttcaatcct ttccgcgtgc tggcgctgag ctatgccata ggcgctatat gcatcgtgat
    49861 gattggctta agccaggatg gattatggtt gatggcgctg gcgattttcg gcactggcat
    49921 tggcattagc ggatcgcagg tggggctgaa tgcgctgacc gcaacacttt atccaacaca
    49981 aagtcgcgcc acgggcgtga gctggtcaaa tgctatcggc cgctgcggcg cgattgttgg
    50041 ttcgctgtct ggcggcgtga tgatggcgat gaatttctct ttcgacaccc tgtttttcat
    50101 tatcgctgtg cctgcggcga tcagtgcggt gatgttaacc ctgctgatta ccgtggttcg
    50161 ccagtcgacc tctgtccctg actcactgcc tcgcgcaggc gttgtgaacg aataaggagc
    50221 atcccaatga ctgataacaa tcaaaatagc cgcgagcaat tttatcagca tatttccggg
    50281 caaaacctga ccccgctgtg ggagtcactg caccatctgg tgccgaaaac gcccaacgct
    50341 aactgtgcgc cagcgtactg gaattatcag gagatccgcc cgctgctact ggagagcggc
    50401 ggcctgattg gtgcgaaaga ggccgtgcgc cgcgtgctgg tgctggaaaa cccggcgctg
    50461 cgcgggcagt cttccattac cgcgacttta tatgccggtt tgcaactgat catgccgggc
    50521 gaagtggcac cgagtcatcg tcataatcag tcggcgctgc gttttattgt tgaaggcaaa
    50581 ggggcattta ccgccgttga tggcgaacgc acgccaatga atgaaggcga ttttatcctg
    50641 accccgcagt ggcgctggca cgatcacggt aaccctggcg acgaaccggt tatctggctc
    50701 gatgggctgg atctgccatt agtgaatatt ctgggctgcg gttttgccga agattatccg
    50761 gaagagcaac aaccggtaac gcgtaaagag ggagattatc tgccgcgtta cgctgccaat
    50821 atgttgccgc tgcggcatca gacggggaac tcctcgccga tctttaacta tcgttatgac
    50881 cgcagccgcg aagtgctgca cgatttaacc cgactgggcg atgccgacga gtgggatggc
    50941 tacaaaatgc gctacgtcaa cccggtcacc ggcggctatc cgatgccgtc gatgggcgct
    51001 ttcctgcaac tgctgccgaa agggtttgcc tcacgcgttg cgcgtaccac tgacagcacc
    51061 atctaccatg tggtggaagg tagcgggcag gtcatcatcg gcaatgaaac cttcagcttt
    51121 agtgcaaaag atatcttcgt ggtgccgacc tggcatggcg tgtcgttcca gaccacgcaa
    51181 gattccgttt tatttagttt ttcggacaga ccggtacaag aagccctggg gctgttccgc
    51241 gaagcgcgtt attaatttag gagagaaaaa atgactcaat acgtattcga accgcaagcg
    51301 cccgttaccg taccggttgt tggcagtgat gaacaattcc ccgtgcgccg cgtttactgc
    51361 gtcggacgta actatgccgc tcacgcccgc gaaatgggct ttgatccgga tcgtgaaccg
    51421 ccattcttct tctgcaaacc ggcggatgcc gttgttccgg ttgccgctgg tgaaacgctg
    51481 gcgctgccgt atccggcaca gaccgataac taccattacg aaattgaact ggtggtggcg
    51541 attggtaaaa aaggcagcga tatcccgctg gaacacgccc atgaatatgt ctggggttat
    51601 gccacgggtc ttgatatgac tcggcgtgac cgtcaaatgg aaatgcgcca gatgggccgc
    51661 ccgtgggaaa tcggtaaagc gtttgatctc tcagcgccca tcgcgccgct gcacaaagcc
    51721 cgcgatatcg gcggtattga taatgcgcca atctggttac aggttaacgg cgaagatcat
    51781 cagcgcagcg atattcgtca tctcatctgg tcggtgaatg aaaccatcag ctatctgtca
    51841 ggcttctttg agctgcaacc gggcgatctg atcttcaccg gtacgccgga aggggttggc
    51901 ccggtagtga aaggtgatgt gatcacaggc aacgtcgagg gcttaacgcc tatcgcggtg
    51961 aagattgtct gaggtgcatg atgaagctgt acagtttttt taatagttca gcgtcgtacc
    52021 gggtacgcat tgcgctggcg ctgaaaggca ttaattacca gaccgaaggg gtgaatatcc
    52081 gtattggtca gcaaaatgaa ctggcctatc ggcggatgaa tccggtcggg ctggtgccga
    52141 cgctgctgac tgacgagggc gaatcactcg gacaatcgct ggcgattatt gactggctgg
    52201 agaggcacta cccgcaagtt ccgctggtgc cgcaggaaga accggcccgc aacaaggtgc
    52261 tggaaattgt ctatgccatc gcttgtgata ttcatccatt gaataacttg cgcgtgctgc
    52321 gctacctgac cgaagaactg aacgtcagtg aagaagaaaa aaaacgttgg tatgcgcact
    52381 ggatccagca agggttaagc gcggtggaac aactgttgcg ccagaaccag tcggggcaat
    52441 tttgtgtggg cgagacgccg acgctggcag attgctgcct tgtcccgcag tgggcgaacg
    52501 cgctacgcat gaactgcgat ttgagcgggt atccacgctg caaagcggtg tacgacgcct
    52561 gcacacagct gccagcgttc attgccgcag cgccagaaaa tcaacaagat aaaatttcag
    52621 cctgaccagg agaactataa tgacgaaagt gacacgcgca gtaattgtgg gaggcgggat
    52681 cggcggtgcg gcaactgcgc tgtcactggc ccgcctgggg atcaaagtgg tgctgctgga
    52741 aaaggcgcat gagatcggtg agattggcgc gggcatccag ttggggccaa atgcgttttc
    52801 ggcgctggac agcctcggcg tcggcgaagt tgcccgccag cgcgcggtgt ttaccgatca
    52861 cattaccatg atggatgcgg tgaacgccga agaagtggtg tgcattgaaa ccgggcaggc
    52921 ttttcgtgac catttcggcg gcccttacgc ggtgatccac cgggttgata ttcacgcctc
    52981 ggtgtgggaa gcggtactga cgcatccgga cgtggaatat cacacctcca ccaacgtcgt
    53041 ggatattcgc gagacggaag acgatgtcac cgtgtttgac gatcgcggca atagctggac
    53101 cggcgatatt ctgattggct gtgacggcgt gaagtcagtg gtgcgccaga gcttattggg
    53161 cgatgcgccg cgtgtcaccg gacatgttgt ctaccgtgcg gttatcgatc gtgccgatat
    53221 gccggaagat ttgcgcatta acgcaccggt actgtgggcg gggccgcatt gtcacttggt
    53281 gcattaccct ttgcgcggcg gcaagcagta taacctggtg gtaacattcc acagccgcca
    53341 gcaggaagag tggggcgtga aggatggtag taaagaggaa gtgctctctt actttgaagg
    53401 gattcacccc cgtccgcgcc agatgttgga taaaccgact tcatggcgac gctggtcaac
    53461 cgctgaccgt gaaccggtgg cgaagtgggg aacgaagcgc ataacgcttg tgggcgattc
    53521 ggcccatccg gtggcgcaat atatggcgca gggagcctgt atggcgctgg aagatgcagt
    53581 aacgctgggt aaggcgctgg cgcagtgtga gggtgatgcg gcgcaagcct ttgcgctgta
    53641 tgagtcggta cgaattccgc gcaccgcgcg catcgtctgg tcgacccgtg aaatggggcg
    53701 ggtttaccac gccgcagggg tagaacgcca ggtacgtaat ctgctgtgga aagggaaatc
    53761 gcaggcagaa ttttatcgcg gcatggagtg gttgtacggc tggaaagaag ataactgttt
    53821 gcaaccacgc tgagcacttc ttcacccaat caggcggtcc cctcgcccct tcgggagagg
    53881 gttagggtga gggtaaaaat cttgcccgaa ttaatcagta agtcagtttg tctgcaaaca
    53941 caggaggcgc taacatagcg cctcatttat ttgcgggtga tgatatgcgt gtgttactgg
    54001 caccgatgga gggagtgctt gactctctgg tgcgtgaatt gctgaccgaa gttaacgact
    54061 acgatctgtg catcaccgag ttcgtccgcg tggtggatca actgctgccg gtaaaagtct
    54121 ttcatcgcat ttgccctgag ctacaaaacg ccagccggac accatccggt acgctggtgc
    54181 gcgtgcagtt gttgggtcag ttcccacaat ggctggcaga gaacgccgcc cgtgcggtgg
    54241 aattaggttc ctggggcgtg gatctcaatt gcggctgccc gtcgaaaacg gtcaacggta
    54301 gcggtggtgg ggcgacgtta ctcaaagatc ctgaactcat ctaccagggt gcaaaagcga
    54361 tgcgtgaagc tgtaccggcg catttgcccg tcagcgtgaa agtgcgtctg ggctgggaca
    54421 gcggtgagaa gaaatttgaa atcgccgatg cggttcaaca ggctggcgct acggagctgg
    54481 tggtgcatgg gcggacaaaa gagcagggtt accgcgcgga gcatattgac tggcaggcga
    54541 ttggtgagat tcgccagcgg ctgaatattc cggtgattgc caacggtgaa atctgggact
    54601 ggcagagcgc gcaacaatgc atggcgatca gcggctgcga cgcagtgatg attggtcgcg
    54661 gggcgctcaa tattcccaac ctgagccggg tagtaaaata taacgaaccg cgaatgccgt
    54721 ggccggaggt ggttgctttg ctgcaaaaat atacccgtct ggaaaagcag ggcgataccg
    54781 ggttatatca cgtagcgcgg attaaacagt ggttgagtta tttgcgtaaa gaatacgatg
    54841 aagcaacgga attatttcag catgttcggg tgttgaataa ttcccatgat attgcaaggg
    54901 ctattcaggc aattgatatc gataagctgc gataatagtg gaatcgcagt ttatcagaga
    54961 caatgaatcg cttattcagc ttcatcatct tgttgcgctc ctttcatgaa ctaagccaca
    55021 tattgccact ggcgcaagga gcgcgcaggg ggaggacaat cgcctcctcc ccctgcaccc
    55081 ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc ccttcggcta
    55141 ccgggtcggg cgcgaggaaa cgtccttgta agacgcgccc tcagcccaca tccatgtggg
    55201 ctgccccggc cttcggggaa cgcttcagcg attttgacgc caccaacacc ggagctgttt
    55261 gcaaatcaag tacacaaaat atctagttcc tgacctgaca acttatatgt catatctgat
    55321 ttttaaaata tcatcttaaa tacgccagtc accaccagta gcccaatcag gaaaataatc
    55381 aatacagccc agagtataat tttcattggc gttcctttat ttgtgagtgc tgaataagta
    55441 taggaaaaaa cgaagccctt tgccgttatc cataaacgat taagataaat cggaagatat
    55501 cattcactta gtgtatattt ttctggatat gtcgttttat tgctactgat gtattgccac
    55561 gcgaaactaa aaaatatatc gcaacacacg gtaatctccg cgcacctttt cttacctgat
    55621 tattccccca tgaatcgtga ttccttttat ccagccatcg cctgttttcc gctgttactg
    55681 atgctgaccg ggtgtgcgcc tatgcatgaa acccgccagg cgttaagcca gcaaacgccc
    55741 gctgcacaag ttgataccgc attacccacg gcgctgaaaa atggttggcc agacagccaa
    55801 tggtggctgg agtatcacga taatcaactc acttcattaa ttaacaatgc actacagaac
    55861 gcaccggata tgcaggtggc tgaacagcgc attcagctag ctgaagccca ggcgaaggca
    55921 attgccactc aggatggtcc gcagatcgac ttttctgcgg atatggagcg gcaaaaaatg
    55981 tcggcagaag ggctaatggg gccgtttgct ctgaacgatc cggccgcagg tacgaccggt
    56041 ccgtggtaca ccaacggaac ttttggctta acggcgggct ggcatctcga tatctgggga
    56101 aagaatcggg cggaggttac tgcccgcctg ggtacggtta aagcacgggc ggcggaacgc
    56161 gagcaaaccc gccaattgct ggctggcagc gtagcccgcc tgtactggga gtggcaaacc
    56221 caggcggcgt taaacacggt cttgcagcaa atcgaaaaag agcagaacac cattatcgcg
    56281 accgatcgcc agctatatca gaacgggatt acttcttcag ttgaaggtgt ggaaaccgat
    56341 attaatgcca gcaaaacccg gcagcagctc aacgatgtcg cggggaaaat gaaaattatt
    56401 gaggcacggt taagcgcact tacaaatcac cagacaaagt cattgaagct taaaccggtc
    56461 gcgttgccga aagtggcaag ccagcttcct gatgaactgg ggtactcctt actggcccgg
    56521 cgggcagatt tgcaggcggc gcactggtac gttgagtcat cgctaagcac cattgatgcg
    56581 gcaaaagcgg cattttatcc tgacatcaac ctgatggcct tcctgcaaca ggatgcgttg
    56641 cacttaagcg atctgttccg tcattccgcg cagcaaatgg gcgttacggc aggcctgacg
    56701 ctacccattt tcgatagtgg tcgtcttaac gccaatctcg atatcgcaaa agccgaaagc
    56761 aacttgtcta tcgccagcta caacaaagcg gtggttgaag cggtgaatga cgtggcgcgg
    56821 gcagccagtc aggttcagac actggcggag aaaaaccagc atcaggcgca aattgagcgc
    56881 gatgccttgc gtgtggtagg tcttgcgcag gcccgcttta acgcgggcat cattgctggt
    56941 tcccgcgtca gcgaagccag aatccccgct ctgcgtgagc gggccaatgg cctgttattg
    57001 caagggcagt ggctggatgc ctccattcaa ctcactggtg cgttgggcgg gggttacaaa
    57061 cgctgatgac atgtgacttc tatacttaac gtttattcag cgttaagtgg agaactcgat
    57121 ggcacaggtc gcgataatta ccgcctccga ttcggggatc ggcaaagagt gcgcattatt
    57181 attggcgcag caggggtttg atattggcat tacctggcac tctgatgaag aaggggcaaa
    57241 agataccgcg cgtgaggtgg ttagccacgg cgtacgtgcg gagatcgtgc aactggatct
    57301 cggcaatctg ccagaaggtg cgcaggcgct ggagaaactc attcaacggc tggggcgtat
    57361 tgatgtgctg gtgaataatg cgggcgcaat gaccaaagcg ccgtttcttg atatggcttt
    57421 cgatgagtgg cgcaagattt ttaccgttga tgtcgatggt gcatttttat gctcacaaat
    57481 tgcggcccgc cagatggtga aacaagggca gggcggtcgc atcatcaaca ttacgtcggt
    57541 acatgaacat acgccgctgc cggatgccag cgcctacaca gccgctaaac atgcgctcgg
    57601 tgggttaacc aaagcgatgg cgctggagct ggtcagacat aagattttgg tgaacgcagt
    57661 cgcgcctggg gcgatcgcca cgccaatgaa tggcatggat gacagcgacg tgaagcccga
    57721 cgcggaacct tcgattcctc tgcggcgttt tggcgcaacg catgagattg ccagcctggt
    57781 agcgtggctg tgctcagagg gcgcaaatta caccaccggg cagtcgttga tagtggatgg
    57841 cggctttatg ttggcgaatc cgcagttcaa cccagaatag cgcagtgagg tgcgtggtta
    57901 tttcaatggg taaaattttg gtaggcatga taagacgcac aagcgccgca tcaggcatgg
    57961 tgcaccggat gtcggatgcg gtacgcacgc cttatccaga gcaatggttt tacgcctgat
    58021 tatccggctt tttcttcccg cgccgtttca gccaccagcg cacgcctacc accagaacca
    58081 cgaccagaat caaccagacc cagtgcttca aatgctgatc gagattgtgc aaccacggcg
    58141 caatcacctg accaccagcg taaccgatag tggtaaaaat caacgcccag gcaaatgcgc
    58201 cgaggatatt cagtggcaga aagattttcg gcggcagctg gctggcacca atcagcgtcg
    58261 ggccaatgac ccgaaagcca tacataaagc gcgtaccaat gacaaacaga tacggatggc
    58321 gttggatcag tttctgcgcc cgctcaattt tatcctgatg tttcgagaaa cggcgtaaca
    58381 gcttgccgcc aaaccgccgc ccgcacagat agagcacctg gtcgccaatc atgccgccaa
    58441 gcgctacaga aagtaccacc agcgggaact ttaatagccc ctgatgtgcc gcaacgcctc
    58501 ccagcaaagt cacggtttca ccttccgcca ggctaccgat aaccagcgcg gcataaccat
    58561 attgtgagat gagtgtattg agatccatag gccagtctgt ctccctttgg ttgcctgtaa
    58621 gcatacaccc tgtgaatcat tcacgctgaa aaaggacgta catcagacgg tgcaaattac
    58681 atgagcgaac aaaaaataat cacaagctga cttatacttg aactgtcagg ttcatgaact
    58741 cacaacaagg gggcggtatg agccatgtct ggggactgtt ttcccatccc gatcgtgaaa
    58801 tgcaggtgat taatcgcgaa aacgaaacga tttctcacca ttacacccac cacgttttgc
    58861 tgatggcggc gattccggtg atttgcgcct tcattggcac tacacagatt ggctggaatt
    58921 ttggcgatgg cactatcctg aagttatcct ggtttaccgg actggcgctg gctgtcctgt
    58981 tttatggcgt tatgctcgct ggggtcgcgg tgatggggcg ggtcatctgg tggatggcgc
    59041 gtaattatcc gcagcgtccg tcacttgcgc actgcatggt cttcgcgggc tacgttgcaa
    59101 ctccgctgtt tttaagcggt ctggtggcgc tttatccgct ggtctggttg tgtgcgctgg
    59161 tcggcacggt ggcactgttt tacaccggtt acctacttta ccttggtatt ccgagcttcc
    59221 tgaatatcaa caaagaggaa ggccttagct tctccagttc gacgctggcg attggcgtgc
    59281 tggtactgga agtgctgctc gcccttaccg ttatactatg gggctacggt tatcgtttgt
    59341 tctagaaagt tctttgacgt tgcattgctg gcgtaaatgt cagcaatgca gcatttcctc
    59401 acgattctcc tttgacgatc gcactttttg ctcgttatga tggcgtcgct agcctcagta
    59461 aatccttact ccggcggtgc gcaacccgtg cgcgtgaacc actatctgaa tgctcatcat
    59521 gccgaaattt cgagtttctt tatttagcct ggccctgatg ctggctgtgc cttttgcacc
    59581 gcaggccgtt gcgaaaacgg cagccgctac caccgcttca caaccggaaa ttgcctccgg
    59641 tagcgcgatg attgttgatc tgaataccaa caaagtgatc tattcgaacc acccggatct
    59701 ggtgcgtccg attgcgtcta tcagcaaatt aatgactgcg atggtcgtgc tggatgcacg
    59761 actgccactg gatgaaaaac taaaagtgga tatcagccag acgccggaga tgaaaggggt
    59821 ctattcgcgc gtacgactga atagcgaaat cagccgtaaa gatatgctgt tgctggcgct
    59881 gatgtcttca gaaaaccgcg cggcggcaag ccttgcgcac cattatcccg gtggttacaa
    59941 agcctttatt aaggcgatga atgcgaaagc taaatcactc ggaatgaaca atacgcgctt
    60001 tgttgaaccg accggattgt cggtgcataa cgtttcaacc gcccgtgact taaccaaact
    60061 gctcattgcc agcaaacaat atccgttgat cggtcagtta agtactaccc gggaagatat
    60121 ggcaaccttc tctaacccga cgtatacgct gccgttccgc aatactaacc atctggtgta
    60181 tcgcgataac tggaatattc agttaaccaa aaccggcttt accaatgcgg cgggccattg
    60241 tctggtgatg cgtacggtta tcaataataa accggtggcg ctggtagtga tggatgcgtt
    60301 tggcaaatat acccattttg ccgatgccag ccgcctgcgt acctggattg aaaccggtaa
    60361 agtgatgcct gtgccggcag cagcgttgag ctataaaaaa caaaaagccg cccaaatggc
    60421 ggcggcgggg cagacggcac agaacgatta agtgaattac ggatggcgat actctgccat
    60481 ccgtcatttt tactccactt cctgccagtt tttccgctta ctggttttac cgatccccgg
    60541 attcatactg ttggtcggat cgttctcgcg ataaaacttc tgcaacgtct ccggtgcttt
    60601 atacaaatga ccgacgttat gttcggcagg gtattgcgcg ccgcgctgct gtagcagttc
    60661 cagcatctgt tctttcaacg catgcacatc cacgcctttt ttaacgatgt aatcctgatg
    60721 gaagacatag cacataaaat ggccgtaata gagcttgtgc accagctggc tgtcgatctc
    60781 cggcggtaaa tgctcatacc actcggtgtc gttacgccgc agagcgatat ccagcgccag
    60841 aatgtcttcg acttcatcgg aatgcaccgc ctgataacga attgctgcgc ccgcagcggc
    60901 gaaacggtgt aaaaacgctt tgctgccttc ctccggcgta cagacaaaga aatcgccttc
    60961 ggcctgtttg aaataatcca ccagccacga tttggcttcg cccacgccat cgcccgccat
    61021 ttttaacagc agatgatgct cgtatttatc gcgccagttt ttcatgcgcg gcggtaaatg
    61081 gctggggaac aggtgaccga atttttgcat cgcacggtca gtaaaatgcg gacggaagaa
    61141 tttcactttc tccagcatcg catcggtgcg tcccttgaga ttaaagaaga acggcatctt
    61201 gtcggtgcct aacttatcaa tcatcaggaa ggtgtctttg ccgtattttt ccgcaatatc
    61261 gtagatatcc cggtgcatat attccccggc aaccggcaga ttttcaaagt tagccagaat
    61321 atgacggcgg atttcggtca gcacttccgg ctggttggtg ccgatataaa acacttgctg
    61381 atttttttcc gcttcgaagg tatcaagacg tactgcaaag acggccagct tcccggcgca
    61441 accagaagat tcaaataacc gatccggatc ggcgttataa cgtgcgggcg tgtcggcttc
    61501 aatatcacga acgcggtgga catagtcata atcgtgggcg tgacgaccat cgtgacgcac
    61561 atcgtcatct ttgatgcgat cgtcatccag cttgctaagg atctgctccg gcgtttcgcc
    61621 cagatcaatc cccagatggt tcaccagcgt cagtttgcca tcttcattta tacgcgcgaa
    61681 taacgacatt tcggtatacg ccgggccacg ctgcaccagc gagccgccgg agttgttaca
    61741 aataccgccg atgaccgatg cgcctataca cgatgatcca atcaccgagt gcggttcgcg
    61801 tcccagcggt ttgagggctt tttccagcga ataaagcgtg gtgcccggat aggccagcac
    61861 ctgttcgccc ttgccgagaa cgtgcagctt gtcgagacgc agggtgctga tgatcacgat
    61921 atcgcgatca taatcgttac cgtttggcgt cgatccttcg gtcaggcctg tattggcagc
    61981 ctgcatcaga ataattttgt cggcggtgac gcaggctttc agcacccgcc acaattccag
    62041 tagtgagcca gggaaaacga cagccagcgc gtcgccctga ccagaacgga agcccttgcg
    62101 atagcgggcc gtttttgcgg gatcggtgag caggtgtgaa tgaccgacca gacgggcaag
    62161 ttcattcaaa aaggctttat tatcagttgt tgtcatggaa gacattttcc actccttgtg
    62221 gtggcgaaaa aatatcacaa actagaatag cgccttgtat gacaattcag cgagtgaata
    62281 ttttgaaaat tcagacaata aactgagcat tctttttctc tctccatcat gcttatggca
    62341 aactacggtt tttgcaccat tttcgctgcc acgtcgggca acaaaggaag aaaaatccat
    62401 atgaaatggc tatgttcagt aggaatcgcg gtgagtctgg ccctgcagcc agcactggcg
    62461 gatgatttat tcggtaacca tccattaacg cctgaagcgc gggatgcgtt cgtcaccgaa
    62521 ctgcttaaga aaatgacagt tgatgagaaa attggtcagc tgcgcttaat cagcgtcggc
    62581 ccggataacc cgaaagaggc gatccgcgag atgatcaaag acggtcaggt tggggcgatt
    62641 ttcaacaccg taacccgtca ggatatccgc gccatgcagg atcaggtgat ggaattaagc
    62701 cgcctgaaaa ttcctctttt ctttgcttac gacgtgctgc acggtcagcg cacggtgttc
    62761 ccgattagcc tcggtctggc ctcgtctttt aacctcgatg cagtaaaaac ggtcggacgt
    62821 gtctctgctt atgaagcggc agatgatggc ctgaatatga cctgggcacc gatggtcgat
    62881 gtctcgcgcg atccgcgctg gggacgtgct tccgaaggtt ttggcgaaga tacgtatctc
    62941 acctcaataa tgggtaaaac catggtggac gcgatgcagg gtaaaagccc ggcagatcgc
    63001 tactcggtga tgaccagcgt caaacacttt gccgcatacg gtgcggtaga aggcggtaaa
    63061 gagtacaaca ccgtcgatat gagtccgcag cgcctgttta atgattatat gccgccgtac
    63121 aaagcggggc tggacgcagg cagcggcgcg gtgatggtgg cgctgaactc gctgaacggc
    63181 acgccagcca cctccgattc ctggctgctg aaagatgttc tgcgcgacca gtggggcttt
    63241 aaaggcatca ccgtttccga tcacggtgca atcaaagagc tgattaaaca tggcacggcg
    63301 gcagacccgg aagatgcggt gcgcgtggcg ctgaaatccg gaatcaacat gagtatgagc
    63361 gacgagtact actcgaagta tctgcctggg ttgattaaat ccggtaaagt gacgatggca
    63421 gagctggacg atgctgcccg ccatgtactg aacgttaaat atgatatggg gttgtttaac
    63481 gacccataca gccatctcgg tccgaaagag tctgacccgg tggataccaa tgccgaaagc
    63541 cgcttgcacc gcaaagaagc gcgtgaagtg gcgcgtgaaa gcctggtgtt gctgaaaaac
    63601 cgtctcgaaa cgttaccgct gaaaaaatcc gcaaccattg cggtggttgg cccgctggca
    63661 gacagcaagc gtgacgtgat gggaagctgg tcggcggcag gtgttgcaga tcaatccgtg
    63721 accgtgctga ccgggattaa aaatgccgtc ggtgaaaacg gtaaagtgct gtacgccaaa
    63781 ggggcgaacg tcaccagtga caaaggcatt atcgatttcc tgaatcagta tgaagaagcg
    63841 gtcaaagtcg atccgcgttc gccgcaagag atgattgatg aagcggtgca gacggcgaaa
    63901 caatctgatg tggtggtggc tgtagtcggt gaagcacagg ggatggcgca cgaggcctcc
    63961 agccgtaccg atattactat tccgcaaagt caacgtgact tgattgcggc gctgaaagcc
    64021 accggtaaac cgctggtgct ggtgctgatg aacgggcgtc cgctggcgct ggtgaaagaa
    64081 gatcagcagg ctgatgcgat tctggaaacc tggtttgcgg ggactgaagg cggtaatgca
    64141 attgccgatg tattgtttgg cgattacaac ccgtccggca agctgccgat gtccttcccg
    64201 cgttctgtcg ggcagatccc ggtgtactac agccatctga acaccggtcg tccgtataat
    64261 gccgacaagc cgaacaaata cacttcgcgt tattttgatg aagctaacgg ggcgctttat
    64321 ccgttcggct atggtctgag ctataccact ttcaccgtct ctgatgtgaa actttctgcg
    64381 ccgaccatga agcgtgacgg caaagtgacg gccagcgtgc aggtgacgaa caccggtaaa
    64441 cgcgaagggg ccacggtagt gcagatgtac ttgcaggatg tgacggcttc catgagccgt
    64501 ccagtgaagc agctgaaagg ctttgagaaa atcaccctga agccgggcga aactcagacc
    64561 gtcagcttcc cgattgatat cgaggcgctg aagttctgga atcaacagat gaaatatgac
    64621 gccgagcctg gcaagttcaa tgtctttatc ggcactgatt ccgcacgcgt taagaaaggc
    64681 gagtttgagt tgctgtaatg ctttagcaag ggcgcgacgt ttgcgccctt tgcctgatgt
    64741 tcatatctta acttgctgaa ttttcgccct ttgtaggccg gataaggcgc tcgcgccgca
    64801 tccggcagga aacgccggac caaatatgcc tcttaagtat ttatctgatt ctgcaactga
    64861 atccgtccgc tcaggctaat cccgctatta tcaactattc ttttctctta attcgctgaa
    64921 aacaacagta aaagaatgag gaaagcatca tgccactctc aaaggtctgg gcaggttcac
    64981 tggttttgtt ggcagccgtg agcctgccgc tgcacgcggc ttcccccgtt aaagtcggtt
    65041 caaaaatcga taccgaaggc gcgctgctcg gcaatatcat tttgcaagta ctggaaagcc
    65101 acggcgtacc gacggtcaat aaagtgcaac tcggaacgac tcctgtggtg cgcggggcga
    65161 ttacttccgg tgaactggat atctatccgg aatataccgg taatggcgct ttcttcttta
    65221 aagatgagaa cgatgcggca tggaaaaacg cgcagcaagg ttacgagaaa gtcaaaaaac
    65281 tcgatgcaga gcaaaacaag ttaatctggc tgacgcccgc accagcgaat aacacctgga
    65341 ccatcgccgt gcgtcaggat gtggcagaga aaaacaaact cacttcgctt gctgacctga
    65401 gtcgttatct gaaagagggc ggcaccttca aactggcagc ctcggcagag tttatcgaac
    65461 gcgccgatgc cttacccgcg tttgaaaaag cctatggctt taagctcggt caggatcagt
    65521 tgctgtcact ggctggtggc gacacggcgg tgacgatcaa agccgctgcc cagcaaacct
    65581 ctggcgttaa tgccgcaatg gcttacggca ccgacggtcc ggtcgcggcg ctggggctgc
    65641 aaaccttaag cgatccgcaa ggcgtgcaac ctatctacgc gcctgcacca gtggtgcgtg
    65701 aatcggtgtt gaaggagtat ccgcaaatgg cacagtggct acagccagtc ttcgccagcc
    65761 tcgatgaaaa aacattgcag caactgaatg ccagcattgc agtggaagga ctggatgcca
    65821 aaaaagtggc tgccgactac ctgaaacaaa aagggtggac gaagtaattt cccgtgactt
    65881 atctacgtat taatcctgtt ctggcgctgc tgctgttgct gacggcaatc gcagcggcgc
    65941 tgccgtttat cagttacgcg cctaatcgtt tagtctcggg tgaagggcgt catctctggc
    66001 aactgtggcc gcaaacgatc tggatgctgg tgggcgttgg ttgcgcctgg ctgacagcct
    66061 gttttattcc cgctaaaaaa ggcagcattt ttgcactcat tctggcgcaa ttcgtcttcg
    66121 tattgctggt gtggggagct ggaaaggcgg cgacccaact ggcgcaaaat ggcagtgcgc
    66181 tggcgcgtac cagtctcggc agtggtttct ggctggctgc ggcgctggca ttgctggcct
    66241 gtagcgatgc catccgccga atctccacgc atccgctgtg gcgctggttg ttgcatatgc
    66301 agattgccat tataccgctg tggttgctgt actccggcac gcttaacgat ctctcactaa
    66361 tgaaagaata cgccaaccgt caggatgtgt ttgacgacgc gctggcacaa catctgacgt
    66421 tgctgtttgg tgcggtgctg cctgcgttag tgattggtgt gccattgggc atctggtgct
    66481 acttttccac cgcgcggcag ggggcgattt tttctctgct caatgtcatt cagaccgtgc
    66541 cttcggtggc gctctttggc ctgttgattg cgccgcttgc cgcgctggtg acggcctttc
    66601 cgtggctggg gaagctcggc atagcaggaa ccggaatgac acccgcactg attgcgctgg
    66661 tgctctatgc cttgctgccg ctggtgcgcg gcgtggtagt cggcttgaac cagatcccgc
    66721 gcgatgtgct ggagagcgcc agagcgatgg gcatgagcgg ggcgcagcga ttcctgcatg
    66781 ttcagttacc gctggcgtta ccggtatttc tgcgcagcct gcgggtggtg atggtgcaaa
    66841 cggtaggcat ggcggtgatt gcggcgttaa tcggcgcagg cggttttggt gcgctggttt
    66901 tccaggggct gttaagcagc gccattgatt tagtgttgct gggggtgatc ccggtaattg
    66961 ttctggcggt gcttaccgac gcgctgttcg atttgcttat cgcactgctg aaggtgaaac
    67021 gtaatgattg aatttagcca tgtcagcaaa ctgttcggcg cacaaaaagc cgttaacgat
    67081 ctcaatctca attttcagga agggagtttt tcggtgctga ttggcacatc tggttccggc
    67141 aaatccacca ccctgaaaat gattaaccgc ctggtggaac atgacagcgg agagctccgc
    67201 tttgccggag aagaaattcg ctcgctgcca gtactggagt tgcgccgccg tatgggctat
    67261 gccattcaat ctattggcct gtttccccac tggagcgtgg cacaaaacat tgccaccgtg
    67321 ccgcaattac aaaaatggtc gcgggcgagg attgacgatc gtatcgacga attaatggcg
    67381 ctactggggc tggagtcaaa tttacgtgag cgttatccac atcagctttc cggtggtcag
    67441 cagcaacgtg tgggagtagc gcgcgcactg gctgccgatc cgcaagtctt actgatggat
    67501 gaaccttttg gcgcactgga cccggtaacg cgcggcgcgt tgcaacaaga gatgacgcgc
    67561 attcaccgtt tgctggggcg taccattgtg ctggtcacgc atgatattga tgaggcgcta
    67621 cggctggcag aacatctggt attgatggat cacggtgaag tggtgcagca ggggaatccg
    67681 ctgacgatgc tgactcgtcc ggcgaatgat tttgtccgcc agttttttgg acgtagtgaa
    67741 ctgggtgtgc gcctgctttc gttacgtagt gtggcggatt acgtgcgtcg cgaagaacgg
    67801 gcagaaggtg aggcactggc agaagagatg acgctgcgcg atgcgctctc cctgtttgtc
    67861 gcgcggggat gcgaggtgct gccggtggtg aacacgcagg gccagcctag cggcacgctg
    67921 cattttcagg atctgctggt ggaggcgtaa gcgtatgaag atgttgcgcg atccgctgtt
    67981 ctggctcatt gctttgtttg tggcactgat tttctggctg ccttacagcc agccgctgtt
    68041 tgctgccttg ttcccacaac tgccacgacc cgtttatcag caagaaagtt ttgcagctct
    68101 ggcactggct catttctggc tggtgggaat ttcgagtctg tttgcggtga tcattggtac
    68161 tggtgccgga attgcagtca ctcgcccgtg gggcgcggaa tttcgcccac tggtggaaac
    68221 tattgccgcc gttgggcaga cttttccgcc cgttgcggtg ctggcgattg ctgttccggt
    68281 gatcggcttt ggtctgcaac cagcgattat cgccttgatc ctgtacggcg tgctgcccgt
    68341 cctgcaggcg acacttgccg ggctgggagc gattgatgcc agcgtgacag aagttgcgaa
    68401 gggtatggga atgagtcgtg gtcagcgact gcgtaaggtc gagctaccgc tggcggctcc
    68461 ggtgattctg gcgggcgtgc gaacttcggt gattatcaac attggtacgg cgacgatcgc
    68521 ctcaacggta ggggccagca cgctgggtac gcccatcatc atcgggctta gcggatttaa
    68581 taccgcgtat gtgatccagg gggcgttact ggtggcactg gcggcgatca tcgcagaccg
    68641 cctgtttgaa aggctggtgc aggcgcttag ccagcacgca aaataaaggt ataacctgcg
    68701 agcatgacgc caccaattcc gcctaacgcc ataaacagga acagggcgat gaccccaatt
    68761 ttagctatgc gcataatgca ctccttatgt taacgaaagg attgtacagt aaagcgcatt
    68821 tgttaacgaa tcattaaatg ccgagtggga aaatatcatg gccttgttcc tgccaactgg
    68881 tgagttgctg ctgttgggcg gaggttcgat tttcaccgca ccacaccagc aatgtacggc
    68941 cttcgaacag ttcagggcgt agttgattga gcgagtgggc gaggacatca atgcgccatc
    69001 cttgttgact ggcaatccag ccctccagcc acagacgggt ggtatcctga atattccagc
    69061 caaccaccag cgcatcttta ccctgttttt tacgtgccga agccagacaa atggcgatgt
    69121 agttgatcag tacgccgtcg aggatcgcca gcagcgcctg gagagtcggt tgttggcact
    69181 gaagccgtcg gcgcagagga ataaacagat gcgtggtgag cgtctgggcg gggtaatcct
    69241 gaccgcgctc tttgatccac gttcgcaggc tatgcagatt gccgctttgc aagtaagtca
    69301 gtaatgtttc ttgctgatcg cgccagccgt tctgcacatc aacattttca ttactgagca
    69361 gcattttaac tttgctgacc tgcacgccgt tgtcgatcca gcgtttgatc tcgcggatcc
    69421 ggtcaatatc ggcatcgttg aacagccgat gaccgccgtc tgtccgttgc ggtttcagca
    69481 atccgtaacg cctctgccac gcgcgtaacg tgacagggtt aatatcacaa agcaacgcca
    69541 cttcaccaat tgtgtaaagc gccatcgtct cacccttgct cgcgaggtcc cggtttaact
    69601 ttagacgcag ttttgcgaac caggtagttt tgcccgtttt ttgtgcattt atagggtgat
    69661 tttatttttg ccaggcgatt ttgagtgatc gtactcacga attctcattt ttctgcaaga
    69721 gttcaaagaa agttaaacgc aggcaatgta tgttacgcgt tttaaaggga agtgtggttt
    69781 gcgggtatgt acgattttaa tctggtgttg ctgctgcttc agcagatgtg cgttttttta
    69841 gtcattgcat ggttaatgag taaaacgcca ttattcatac cgttaatgca ggtcacggtt
    69901 cgtctgccgc ataagtttct ctgctacatc gtcttttcca tcttctgcat catgggcacc
    69961 tggtttgggt tgcacattga cgattctatt gccaataccc gtgcgatagg cgcggtaatg
    70021 ggcggcttac tcggcggtcc ggtcgtcggt gggctggttg gcctgaccgg cgggttacat
    70081 cgatattcga tggggggcat gaccgcgcta agttgcatga tctcgaccat cgttgaagga
    70141 ttactcggtg gtctggtaca cagcatcctg atccgccgcg ggcgcactga taaagtcttt
    70201 aaccccatta ccgccggtgc cgtcacgttc gtcgctgaaa tggtgcaaat gttgatcatc
    70261 ctggcgatcg cccgacctta tgaagatgcg gtgcgtctgg tgagtaatat tgctgcgcca
    70321 atgatggtca ccaataccgt cggcgcggcg ctgtttatgc gtatattgct cgataaacgc
    70381 gcgatgtttg aaaaatacac ttcagctttt tctgccactg cgctgaaagt ggcggcctcg
    70441 acggaaggca ttttgcgcca ggggtttaac gaagtgaaca gcatgaaagt ggcacaggtg
    70501 ctgtatcagg agctggatat tggtgcagtc gcgattaccg atcgagagaa attgctggcc
    70561 tttaccggaa ttggtgacga ccaccattta cccggcaaac cgatttcttc gacttatacc
    70621 ttaaaagcga ttgaaaccgg tgaagtggtc tacgctgatg gcaacgaagt accttaccgt
    70681 tgctctttgc atccgcaatg caaactgggg tcgacgctgg taattccgtt acgtggtgaa
    70741 aatcagcggg tgatgggcac catcaaattg tatgaagcca aaaaccgttt attcagttca
    70801 atcaaccgca cgttgggtga ggggattgcg caattgcttt cggcgcagat ccttgccggg
    70861 caatatgagc ggcaaaaagc gatgctcacc cagtcagaga tcaaactgct tcacgcccag
    70921 gtgaatcccc attttttgtt taatgcgctt aacaccatta aagcggtgat ccgccgcgac
    70981 agcgaacagg ccagccagct ggtgcagtat ctttccactt ttttccgcaa aaacttaaag
    71041 cggccttcgg agtttgttac tctcgccgac gaaattgaac atgtgaacgc ttatctgcaa
    71101 attgaaaagg cgcgcttcca gtcgcggttg caggtcaaca ttgctattcc gcaagaatta
    71161 tcccagcagc aattgcccgc gtttaccctg caaccgatag tggaaaacgc cattaaacat
    71221 gggacatcac aactgctgga tacagggcga gtggcaatca gcgcccgacg tgaggggcaa
    71281 catttgatgc tggagatcga agacaatgcc ggtttgtatc aaccggtaac caatgccagt
    71341 gggctgggga tgaatctggt ggataagcgt ttacgtgaac ggtttggcga tgactatggg
    71401 ataagcgtcg cctgtgagcc tgatagttac acccgaataa cgttacgact accatggagg
    71461 gacgaggcat gattaaagtc ttaattgtcg atgatgaacc gttagcacgg gagaacctgc
    71521 gcgtattttt gcaggagcag agcgatattg aaatcgttgg tgagtgttca aacgccgtgg
    71581 aaggaatcgg cgcggtgcat aaactgcgcc cggatgtgct gtttctcgat atccagatgc
    71641 cgcgcatcag tggtctggaa atggtgggga tgcttgatcc ggaacatcgc ccgtatattg
    71701 tttttctcac cgcgtttgac gaatacgcta ttaaagcctt tgaagaacat gcctttgatt
    71761 atctgctgaa gccaattgat gaagcgcgac tggagaaaac gctggcgcga ttgcgtcagg
    71821 agcgcagcaa gcaggatgtt tcgctgttac cggaaaatca acaggcgctg aaatttatcc
    71881 cttgtacggg gcatagtcgg atttatttgc tgcaaatgaa agatgtggca tttgtcagca
    71941 gtcggatgag cggtgtctac gttaccagcc acgaagggaa agagggcttt accgaattga
    72001 cattacgtac tctggaaagt cgtacaccac tactgcgctg ccatcgtcag tatctggtta
    72061 acctcgcgca tttacaggag attcgtctgg aagataacgg ccaggccgag ttgattttgc
    72121 gtaatggctt aaccgtgccg gtcagccgcc gttatctgaa aagcttaaaa gaggcgattg
    72181 gcctgtaaaa gactgctaaa atggcttttt gcctcatcaa cacctgaagg cctcatgcta
    72241 agtaacgata ttctgcgcag cgtgcgctac attttgaaag ccaataataa tgacctggtg
    72301 cgtattctgg cgctgggtaa tgtcgaagcc accgcggaac agatcgccgt ctggctacgt
    72361 aaagaagacg aagagggttt tcagcgttgt ccggacattg ttttgtcgtc attcctcaat
    72421 ggcctgattt atgaaaaacg cggcaaggat gagtctgctc cggcactgga gccggaacgt
    72481 cgcattaata acaacatcgt gctgaaaaaa ttacgcatcg cgttttcgct gaaaaccgat
    72541 gacattctgg cgatcctcac cgaacagcag ttccgcgttt cgatgccgga aattacggcg
    72601 atgatgcgtg caccggatca taaaaacttc cgcgaatgcg gcgatcaatt tttacgttat
    72661 tttctgcgtg gactggcagc gcgccagcat gtgaagaaaa gctaagacgg gtatggcggc
    72721 catgcgcaaa catggccgcc gacagattat ttcacttctt taaaaccaga ggctttcatc
    72781 accagttcca tttgcgccat agtgatacct tttttggcat cttcagcaga aacgttgatt
    72841 cctgaaatac cctgcagggc tttaaaatcc actttttcca tatcgatagt cacgttttcc
    72901 tgcgcgtagg tatcggtata ggttaatttt tcttcaacac ccgcgatgtt tttgtatttg
    72961 gcgcttaacg gctcaagtgt cttggcagca tcttctttgg tggttgcacc aatggaggca
    73021 aattgaattt tggtttcaga agattgctta agcaccttgt cacctttgta gatataggta
    73081 atggcaattt cagtgccgtt cagattggcg ctgaatttct tcgattcttc tttgtcaccg
    73141 cagccagcaa gagagaaaac cagaacagat gcaacaacaa gggaaaacag cttattgaaa
    73201 gccttcatgt aaaactccat tttatttaat caaggaactg gtgactctca ccaggggcta
    73261 tataggatat gcctaatacc gtggcgtgag cagtccggaa ctggagtaga actcttagta
    73321 aaaagcacta tttcatcctt gttgctgaag catggggaat aattgttcgc acagcaaaac
    73381 accgttattc attgcttcta cccgtgcctc gctttctgta ttacgaaatt gtcccaacat
    73441 atgtgccagc cgataaaaac ccaccgcggt gaggtcattc gccagcaact ctgcctgacc
    73501 aataacactc tgttcctgat agcgccagcc gttatggagc agttgaataa gtaatgcctg
    73561 gcagcgcatc agcaactgat gagcagtaga cggcacaggc agaacgctga cagaaggtag
    73621 tgatgtcacc acaggcgcag tttctgcgtc cagcgcccag gcacgagtct ttgtcatcat
    73681 tacctgtggt tccagtgtca attgcccatc aacaaaactg acaaagccag aaaccagaca
    73741 cacggggtcg tctgtttgtt gcaaaagcgc cgccatgcgt tcaacggcat aaggcgcgct
    73801 ggctgaggct ggcagtgata acgtcagcag attatcttca ccttcgccgc tgattacctg
    73861 cgcatccagc gtctggcggc tgctatccca accgagagaa atacactcag cgaccggcag
    73921 aataaataag ttatcgacct gattaagagg ccgtatgcag gcggggggac gctggcgtaa
    73981 atattcccgc aaagccacaa tgcccggctg gcgtaacggc gcgctcaaca tttgccaggc
    74041 atcaggcgac agcggcacaa cgctgcttaa gcggttgcgg gtagctaaca gcagctcgcc
    74101 atcggcactg cgttttgctg cttgtgaaac aatttgcccg cccgccagtg cgccagcctg
    74161 aaaactaaac agccgacgcg tagctgccgg tgagttttcc tgttcacttc gtggccaact
    74221 gcgcgaaagg tgcaaaatac tgccggtgtc gggatcggta aaccagatgc gtaaaccata
    74281 ctgctcaata tcctgccagc aacgcatacc taaagacacc agtcgcagat gatcaagctt
    74341 tgcttctccg gcaatgccag cgccaacgac cgtgcgccac ggcacaggag gaacttcacc
    74401 gatactgtcg cgccgcgcca tctcttgtgc gcaatttaat cgactgttta atgccgcaag
    74461 ctgacgtaag cattctcctg catgatagtg gctggcgcgg gcgtggaagg catcaacgct
    74521 ggcgcgcagt tgccgtagtg attcactcac ccagcgccag ttgcagcgtt ccgccgcttg
    74581 ctgcgcgcgg ctgaaagcgg cctcgtagtg aataagcggc tggctgatgc cgcccagcca
    74641 taatgcctgg cttagttgct gaacatattg acgacacgcg ttgccctctt cgctggcaaa
    74701 cggatcgtca gatgatgtga cgtgttcgct gcgcatctgc cagattaaat gagtaaattc
    74761 cgcttgctga gttttggcct cgacgaaggc ctgcacagcc agtacgacat gttcgcaaag
    74821 tgtgccttca atacaatcac aacgggcgaa acgaatgctg ctacgggaat aaaaacgcac
    74881 atcgctcatc ggtaagcggg ctgagggaat ttcgccaggc gcacagaaca actcaatggt
    74941 gatgccttta gcgaccagca cctgtgcacg tttgcgggtg gcatcgggca gggtagccag
    75001 ttcttccagc cagattgcag gctgccactc ttcttctttt tccgtgggcc gggtggtggc
    75061 acaaagtcgc tgataactta acaccagcat cacgcgatga cggcacatgc cgctagcccc
    75121 gcaggtgcac tgagcctctt tcagtgcctg gccgttcgcc agctgggtac ggacaccgtc
    75181 actgaaggtg gcgattaaag cgccgttctc atggctgatt tccgggacgt tgccattttc
    75241 cagttcctta aggctgcgct taacaaaacc ggcattgctt aacgccgtca gggcctgcgg
    75301 tgtcagttct aataattccg gacgtagtga attcatgact gaagattctc cgcaagccat
    75361 gatgccagtt cgcccggtgt catggcggct atttgtgcgc cgacattaac cagcgcctgg
    75421 gccgtatcgc ggtcatagca aggcgttgct gtgctatcga gcgccgccag tcccagcact
    75481 ttgatgccgc tctggacaca ctttttcacc tgatgcgtca gcaatgatga tgaaccccct
    75541 tcgtaaaaat cgctcacgag gataatgacg ctttttgctg gttgttcaat aagttgccga
    75601 ccatattcca cggcactggc gatattggtc ccgccgccca actgtacttt cattaataac
    75661 tctaccggat cggcaacgtc tgccgtgaga tcaacgacgc ttgtgtcaaa cgccaccaga
    75721 tgggtacgaa tgccgggtaa ctgccacaaa caggccgcca tcaccgcaga gtggatcacc
    75781 gagtccacca tcgatccgct ttgatcaacc agtaagacca gttgccattg ttcactgtgg
    75841 cgtttaatgc ggctgttaaa gcggggggat tcgatataca acttgccgtg ttgcgggtgc
    75901 cagtgttgca ggttggcgcg cagagtactt ttgaaatcaa agtttcgcgc cagtggaata
    75961 aatgagcggc gacggcgatc gcggacacca gaaaaagcct gatgaacttc ctttgccagt
    76021 cgagccataa tttcttcaac aacctggcgc actatccggc gggcggcagc cagtacttcg
    76081 gggttcatca gatgtttggt gtgcaaaacg gcgcgtagca ggctttcaga aggctgcata
    76141 cgttccagca cgtcgagatt cgtcaccaca tcttcaatgc cgtagcgcag tacggcatcg
    76201 ctttccagcc gctcaatcac ctgttgcgga aacagcgtgt gaatactgtt gatccactca
    76261 ggggtggtga gatttgagcc acctaatcca ccggagcgtt caccacgctg gagccgttca
    76321 ggatcgcgcc catacagcca ctccagcgcg tggtctatct gccgggagtt gtcatccagc
    76381 ccacaaagcg tcgtttctgc cgcttcgcca agaattaatc gccagcgttg tagctcacgg
    76441 gtggtcagaa gatcgttcag ttcagacatg gaaaactccc cagttttgca gcgatgccag
    76501 tgcctgctgt tcgagttgtt gatgatgtgc aatggcttgt ggtggacaat gcaacggcat
    76561 ttgcagcgcc gaaacgggaa gttgcgccag ttgataatgc tcaagcacct gatgcgccag
    76621 cgtcccgcgt tctcgtggtg gtagccaggc catcgccgcg cgtaaatcgg gcagggcatt
    76681 gataaaattg gcatcactca gttgatttaa atgactgctg aaaccggcga taaatgccgg
    76741 ctgacaggcc agttgatggc gggccagcgc cagcaaaccg tgcagcgctt cgccggattg
    76801 tgccggggat aactgcgcca gcatcgtcag cgccgcttcg gtaatggcgt tgggatgttc
    76861 cagacgcatc agtgcgccaa gagccgcgcc gcgatccagc gccggggcat gaatcgcctg
    76921 actgcgtcgc tccagtaagg ctaccgctgc agaaagagaa acgccgggta aattaacgcc
    76981 gctatgtaga tcgcgcagga tatggcaaag cgcctgccag ctatgcaggt gagcgtgaaa
    77041 ctccttttca tccggtttgc cgttggattc acacagccag agcgtgcgat cgatagccgc
    77101 gcataacgtc gtttgtaata tctgcgcgcc ttgcataccg ctaatttcat ccagccgcca
    77161 taaggcatat agcacttcca gcgcctggcc catttcggca aattgatttt cctgggcgat
    77221 taataatgtg agttgctcca gtaattgttg actgaaggac gccagacccg ctaacgccgc
    77281 ctggctaaga catgtggtga tactgccgat tccacccgcg tccagcatat cggcttctaa
    77341 tttatggcgt gcggcttcct ggagtgtggc accaaagcag gcggcctcaa tcaatgccgc
    77401 atgctggcta agcgggcggg ttaatttcca gcgttcttca ccgttgcctg caagtgtcag
    77461 tgtgcttccc tgctggcgta caatccctgg gatctccagt attgccagcc gatgtaacac
    77521 ctgactttgc gctagtccat tggggttaaa gcgatttaag gtaagctccg ccggaagaga
    77581 gattgcggta cgttccagtt cgcaggtgac atcttttggc agaggcggtt gtggcgtaga
    77641 aggggcaagt ttaccgaatc cgtcacccgc taatgtgtct atcaacgtta gcagaatcgg
    77701 atcggtgtcg ggatgaataa ctccgcgata gctccacggc aacggcgcgt tcagggcttc
    77761 tttaatcagc gagcctgcta tggcatccag ccagtcagtg cgtaacggta gtgtatgacc
    77821 gcgcaactgt gccagcgcca tcgcatgcag atgagcggca gccatatccg cggtcgaagc
    77881 aggcaacttg tgctggcgca aacgggtaag gatagttttt agcagttgtt caccggcctg
    77941 ctgtaagccc cactgccagc accagttttg ccagaccggg gcaggcatac cggaaaggta
    78001 tcctgccagc acatcaaggc gcttttcgct gtatggcgtg agataacaac ctgtaatggc
    78061 atctgccagc gagggcaatt ccggcgtgtt aatttcctgc gggcattcgc gccacatctt
    78121 tgccagtgcc gaagcatgcc agccgccgca gacgactaac acgtcgccat tgttttgctg
    78181 catcgcccat ccaatccagc gggccataaa ggcttcgcgc tgacgattga gcgcatcgcc
    78241 gggggagtcg ccccgcagtt gggcaaaata gcgcgccagt gcagagggta atgcagtttg
    78301 ctggctttca tcttcgaaca agtgatccca cagggtgtcg ctgttatcca tgcgggtggc
    78361 acgcagcagc aaggtctggc tttcctcttg cgtatcaggc gaatcgtcct cttcttcact
    78421 ctgtgcccag caaggcaaat cgatgaagta agtttgtgcc tgaatacgac gagcggcttg
    78481 tagcgcctgc cactccggcg aaaattcagc aaatggcgtc caggcaccac gcccgggggc
    78541 tgcaccgtcc tgatactggc aataactgta aatagctacc ggaagctggt gggctaaaaa
    78601 cagttcgtct acccggtcat taaaatcagc cgggccttca atcaacacgt atcgtggccg
    78661 ctggctttcg attaacgatt tcaccagccg ggcgcaggcc ggactatgat gccggatgcc
    78721 gacaattaac ggctcgctca tgcatctttc ctcacggcag gcggtggcgg gcttgataat
    78781 aagcctgcca gtgagcttct ttatgtgtgg cgacacgctg ttcgaagtaa cggcgcagac
    78841 gagcgcgatc tccttcgtta tctttgacga tggttccggc aatacagtcc accaggtcgg
    78901 caggctcccc cgcgcgattc gctaaaaacc aggcgcggac gcccacggcg tgggccacgt
    78961 tcacggcttc agccgtggac ataatcgctg ttagtgtatc cattgacgtt ttcttttcgc
    79021 cgttggcacg cagatcgcgg aaggtgcgga ccagtaactc cagtaccgcg tcaggcactt
    79081 tatgcggaat accgctatgg gccagcaaac gcgccgaagc actggcgaca agctccagct
    79141 cctgggcgaa atccataatc ggaaagacag tttcaaaatc gaagcgacgc tttagcgcag
    79201 cactcatttc attgacgccg cgatcgcggg tattggcagt ggcaataata ttaaagcctt
    79261 ctcgcgcata gagctgactg gcttcaccag tgagttccgg caccgtcatc accctgtcag
    79321 aaagcatacc taacagacag tcttgcactt ccagcggtgt acgcgttatc tcttcaaaac
    79381 ggacgatttt gccgtcgcgc attccctggt aaagcggggc agggaccagc gcttccgttg
    79441 atggaccatg attgataagc agggcgtagt tccagccgta cttgatttga tcttcggtag
    79501 tagatgcgcc gccctggatg gttaacccgg catcgccgct aatagcggtt gccagtaatt
    79561 cagacaggag agattttgcg gtgccaggtt cccccaccaa catcaaaccg cgtccggtgg
    79621 caagagtgac taacatacgt tcgacagcgg agggactaat cactacttta cggctaatat
    79681 tttgcgcgct atcgccaagg ataaaagcac gggccgcagg caaacttaac tgccaaccgg
    79741 gcgggcaagg tgcggtatca ttttgtttta attttgccag ttcatcggcg tataacacag
    79801 cagcaggcgg acgctgcaga tgattattct gcggtgacat atattcatcc ctgaaatgta
    79861 ttaatgaaat aaacgatatt ttacgcgcaa taaatgtttg gcgcattagt tgtttgagcg
    79921 aaaaatatct ggaaagcaga tcacagtaaa taaaaaaacc tgctggtgag gcaggttctt
    79981 ttatttacta ttttcaggcc taacaccaca gcctgtgcaa catcgtgttc caacgaacga
    80041 attataacca cggctggacc ccgttgcaat taagataatg agatttataa tccagaccgt
    80101 tgggacgttg cgccggatcg aacctatttc aagtcctggc taccggacgg gtggtgctgc
    80161 aggtggtgcc ctgatccgga gtgcaacatc gtggtccaac atacgtaaac cagtatcgac
    80221 aggcagtgta agggtaaggt gctggtgttg cgctccttaa taaggagagc ggatggacac
    80281 gctgacacaa aagttaaccg tgctcattgc tgtactggag ctattagt
//
LOCUS       DAZUCE010000002        80141 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZUCE010000002 DAZUCE010000000
VERSION     DAZUCE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.002"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            41..625
                     /gene="yraH"
                     /locus_tag="ACHY03_000072"
     CDS             41..625
                     /gene="yraH"
                     /locus_tag="ACHY03_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3903365.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGV
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            705..1400
                     /gene="yraI"
                     /locus_tag="ACHY03_000073"
     CDS             705..1400
                     /gene="yraI"
                     /locus_tag="ACHY03_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3903366.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVSDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1429..3945
                     /gene="yraJ"
                     /locus_tag="ACHY03_000074"
     CDS             1429..3945
                     /gene="yraJ"
                     /locus_tag="ACHY03_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS3903367.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKLCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3956..5047
                     /gene="yraK"
                     /locus_tag="ACHY03_000075"
     CDS             3956..5047
                     /gene="yraK"
                     /locus_tag="ACHY03_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3903368.1"
                     /translation="MKRAPLMTGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5090..5950)
                     /gene="rsmI"
                     /locus_tag="ACHY03_000076"
     CDS             complement(5090..5950)
                     /gene="rsmI"
                     /locus_tag="ACHY03_000076"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFS3903369.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6015..8051
                     /gene="lpoA"
                     /locus_tag="ACHY03_000077"
     CDS             6015..8051
                     /gene="lpoA"
                     /locus_tag="ACHY03_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFS3903370.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRLEKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQVRYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWEKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8009..8404
                     /gene="yraN"
                     /locus_tag="ACHY03_000078"
     CDS             8009..8404
                     /gene="yraN"
                     /locus_tag="ACHY03_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFS3903371.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8424..9014
                     /gene="diaA"
                     /locus_tag="ACHY03_000079"
     CDS             8424..9014
                     /gene="diaA"
                     /locus_tag="ACHY03_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFS3903372.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9024..9599
                     /gene="dolP"
                     /locus_tag="ACHY03_000080"
     CDS             9024..9599
                     /gene="dolP"
                     /locus_tag="ACHY03_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFS3903373.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEAHINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9713..10753)
                     /gene="yraQ"
                     /locus_tag="ACHY03_000081"
     CDS             complement(9713..10753)
                     /gene="yraQ"
                     /locus_tag="ACHY03_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFS3903374.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10826..11461)
                     /gene="yraR"
                     /locus_tag="ACHY03_000082"
     CDS             complement(10826..11461)
                     /gene="yraR"
                     /locus_tag="ACHY03_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFS3903375.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11589..12107
                     /gene="yhbO"
                     /locus_tag="ACHY03_000083"
     CDS             11589..12107
                     /gene="yhbO"
                     /locus_tag="ACHY03_000083"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFS3903376.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGYSPDYLRGDNRFVIFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12087..12530)
                     /gene="yhbP"
                     /locus_tag="ACHY03_000084"
     CDS             complement(12087..12530)
                     /gene="yhbP"
                     /locus_tag="ACHY03_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFS3903377.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12581..12883
                     /gene="yhbQ"
                     /locus_tag="ACHY03_000085"
     CDS             12581..12883
                     /gene="yhbQ"
                     /locus_tag="ACHY03_000085"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFS3903378.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKEHLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12870..13373)
                     /gene="yhbS"
                     /locus_tag="ACHY03_000086"
     CDS             complement(12870..13373)
                     /gene="yhbS"
                     /locus_tag="ACHY03_000086"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3903379.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13367..13891)
                     /gene="ubiT"
                     /locus_tag="ACHY03_000087"
     CDS             complement(13367..13891)
                     /gene="ubiT"
                     /locus_tag="ACHY03_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFS3903380.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14100..15095
                     /gene="ubiU"
                     /locus_tag="ACHY03_000088"
     CDS             14100..15095
                     /gene="ubiU"
                     /locus_tag="ACHY03_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFS3903381.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMASNIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15104..15982
                     /gene="ubiV"
                     /locus_tag="ACHY03_000089"
     CDS             15104..15982
                     /gene="ubiV"
                     /locus_tag="ACHY03_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFS3903382.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDLVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16063..17070
                     /gene="yhbW"
                     /locus_tag="ACHY03_000090"
     CDS             16063..17070
                     /gene="yhbW"
                     /locus_tag="ACHY03_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFS3903383.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDIARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDVDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17188..18432)
                     /gene="mtr"
                     /locus_tag="ACHY03_000091"
     CDS             complement(17188..18432)
                     /gene="mtr"
                     /locus_tag="ACHY03_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFS3903384.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18586..20475)
                     /gene="deaD"
                     /locus_tag="ACHY03_000092"
     CDS             complement(18586..20475)
                     /gene="deaD"
                     /locus_tag="ACHY03_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFS3903385.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20468..20548)
                     /gene="yrbN"
                     /locus_tag="ACHY03_000093"
     CDS             complement(20468..20548)
                     /gene="yrbN"
                     /locus_tag="ACHY03_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFS3903386.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20655..21539)
                     /gene="nlpI"
                     /locus_tag="ACHY03_000094"
     CDS             complement(20655..21539)
                     /gene="nlpI"
                     /locus_tag="ACHY03_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFS3903387.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21648..23783)
                     /gene="pnp"
                     /locus_tag="ACHY03_000095"
     CDS             complement(21648..23783)
                     /gene="pnp"
                     /locus_tag="ACHY03_000095"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFS3903388.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24030..24299)
                     /gene="rpsO"
                     /locus_tag="ACHY03_000096"
     CDS             complement(24030..24299)
                     /gene="rpsO"
                     /locus_tag="ACHY03_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFS3903389.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24448..25392)
                     /gene="truB"
                     /locus_tag="ACHY03_000097"
     CDS             complement(24448..25392)
                     /gene="truB"
                     /locus_tag="ACHY03_000097"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFS3903390.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25392..25793)
                     /gene="rbfA"
                     /locus_tag="ACHY03_000098"
     CDS             complement(25392..25793)
                     /gene="rbfA"
                     /locus_tag="ACHY03_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFS3903391.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25958..28630)
                     /gene="infB"
                     /locus_tag="ACHY03_000099"
     CDS             complement(25958..28630)
                     /gene="infB"
                     /locus_tag="ACHY03_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFS3903392.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28655..30142)
                     /gene="nusA"
                     /locus_tag="ACHY03_000100"
     CDS             complement(28655..30142)
                     /gene="nusA"
                     /locus_tag="ACHY03_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFS3903393.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30170..30622)
                     /gene="rimP"
                     /locus_tag="ACHY03_000101"
     CDS             complement(30170..30622)
                     /gene="rimP"
                     /locus_tag="ACHY03_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFS3903394.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLL
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30829..30905)
                     /locus_tag="ACHY03_000102"
     tRNA            complement(30829..30905)
                     /locus_tag="ACHY03_000102"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30869..30871),aa:Met,seq:cat)
     gene            31253..32596
                     /gene="argG"
                     /locus_tag="ACHY03_000103"
     CDS             31253..32596
                     /gene="argG"
                     /locus_tag="ACHY03_000103"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFS3903395.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQMENLENKGQ"
     gene            complement(32604..34229)
                     /gene="yhbX"
                     /locus_tag="ACHY03_000104"
     CDS             complement(32604..34229)
                     /gene="yhbX"
                     /locus_tag="ACHY03_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS3903396.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34688..34774)
                     /locus_tag="ACHY03_000105"
     tRNA            complement(34688..34774)
                     /locus_tag="ACHY03_000105"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34738..34740),aa:Leu,seq:gag)
     gene            complement(34789..35121)
                     /gene="secG"
                     /locus_tag="ACHY03_000106"
     CDS             complement(34789..35121)
                     /gene="secG"
                     /locus_tag="ACHY03_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFS3903397.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35349..36686)
                     /gene="glmM"
                     /locus_tag="ACHY03_000107"
     CDS             complement(35349..36686)
                     /gene="glmM"
                     /locus_tag="ACHY03_000107"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFS3903398.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36679..37527)
                     /gene="folP"
                     /locus_tag="ACHY03_000108"
     CDS             complement(36679..37527)
                     /gene="folP"
                     /locus_tag="ACHY03_000108"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFS3903399.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37617..39551)
                     /gene="ftsH"
                     /locus_tag="ACHY03_000109"
     CDS             complement(37617..39551)
                     /gene="ftsH"
                     /locus_tag="ACHY03_000109"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFS3903400.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39651..40280)
                     /gene="rlmE"
                     /locus_tag="ACHY03_000110"
     CDS             complement(39651..40280)
                     /gene="rlmE"
                     /locus_tag="ACHY03_000110"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFS3903401.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40406..40699
                     /gene="yhbY"
                     /locus_tag="ACHY03_000111"
     CDS             40406..40699
                     /gene="yhbY"
                     /locus_tag="ACHY03_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFS3903402.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40855..41331)
                     /gene="greA"
                     /locus_tag="ACHY03_000112"
     CDS             complement(40855..41331)
                     /gene="greA"
                     /locus_tag="ACHY03_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFS3903403.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41579..43012
                     /gene="dacB"
                     /locus_tag="ACHY03_000113"
     CDS             41579..43012
                     /gene="dacB"
                     /locus_tag="ACHY03_000113"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFS3903404.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGINQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFVRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNDPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43053..44225)
                     /gene="cgtA"
                     /locus_tag="ACHY03_000114"
     CDS             complement(43053..44225)
                     /gene="cgtA"
                     /locus_tag="ACHY03_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFS3903405.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44241..45206)
                     /gene="yhbE"
                     /locus_tag="ACHY03_000115"
     CDS             complement(44241..45206)
                     /gene="yhbE"
                     /locus_tag="ACHY03_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS3903406.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQIVGALMLLSGLVMFFNTSLIEIFTKLTDYTWG
                     VIFGVGAATVWVIYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45333..45590)
                     /gene="rpmA"
                     /locus_tag="ACHY03_000116"
     CDS             complement(45333..45590)
                     /gene="rpmA"
                     /locus_tag="ACHY03_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFS3903407.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45611..45922)
                     /gene="rplU"
                     /locus_tag="ACHY03_000117"
     CDS             complement(45611..45922)
                     /gene="rplU"
                     /locus_tag="ACHY03_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFS3903408.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46181..47152
                     /gene="ispB"
                     /locus_tag="ACHY03_000118"
     CDS             46181..47152
                     /gene="ispB"
                     /locus_tag="ACHY03_000118"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFS3903409.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     DNYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47381..47659
                     /gene="sfsB"
                     /locus_tag="ACHY03_000119"
     CDS             47381..47659
                     /gene="sfsB"
                     /locus_tag="ACHY03_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFS3903410.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47707..48966)
                     /gene="murA"
                     /locus_tag="ACHY03_000120"
     CDS             complement(47707..48966)
                     /gene="murA"
                     /locus_tag="ACHY03_000120"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFS3903411.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49021..49275)
                     /gene="ibaG"
                     /locus_tag="ACHY03_000121"
     CDS             complement(49021..49275)
                     /gene="ibaG"
                     /locus_tag="ACHY03_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFS3903412.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49435..49728)
                     /gene="mlaB"
                     /locus_tag="ACHY03_000122"
     CDS             complement(49435..49728)
                     /gene="mlaB"
                     /locus_tag="ACHY03_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFS3903413.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGVTCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49728..50363)
                     /gene="mlaC"
                     /locus_tag="ACHY03_000123"
     CDS             complement(49728..50363)
                     /gene="mlaC"
                     /locus_tag="ACHY03_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFS3903414.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50382..50933)
                     /gene="mlaD"
                     /locus_tag="ACHY03_000124"
     CDS             complement(50382..50933)
                     /gene="mlaD"
                     /locus_tag="ACHY03_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFS3903415.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50938..51720)
                     /gene="mlaE"
                     /locus_tag="ACHY03_000125"
     CDS             complement(50938..51720)
                     /gene="mlaE"
                     /locus_tag="ACHY03_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFS3903416.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51728..52537)
                     /gene="mlaF"
                     /locus_tag="ACHY03_000126"
     CDS             complement(51728..52537)
                     /gene="mlaF"
                     /locus_tag="ACHY03_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFS3903417.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAKALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52747..53724
                     /gene="yrbG"
                     /locus_tag="ACHY03_000127"
     CDS             52747..53724
                     /gene="yrbG"
                     /locus_tag="ACHY03_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFS3903418.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53738..54724
                     /gene="kdsD"
                     /locus_tag="ACHY03_000128"
     CDS             53738..54724
                     /gene="kdsD"
                     /locus_tag="ACHY03_000128"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFS3903419.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54745..55311
                     /gene="kdsC"
                     /locus_tag="ACHY03_000129"
     CDS             54745..55311
                     /gene="kdsC"
                     /locus_tag="ACHY03_000129"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFS3903420.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55308..55883
                     /gene="lptC"
                     /locus_tag="ACHY03_000130"
     CDS             55308..55883
                     /gene="lptC"
                     /locus_tag="ACHY03_000130"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFS3903421.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55852..56409
                     /gene="lptA"
                     /locus_tag="ACHY03_000131"
     CDS             55852..56409
                     /gene="lptA"
                     /locus_tag="ACHY03_000131"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFS3903422.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56416..57141
                     /gene="lptB"
                     /locus_tag="ACHY03_000132"
     CDS             56416..57141
                     /gene="lptB"
                     /locus_tag="ACHY03_000132"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFS3903423.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57189..58622
                     /gene="rpoN"
                     /locus_tag="ACHY03_000133"
     CDS             57189..58622
                     /gene="rpoN"
                     /locus_tag="ACHY03_000133"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFS3903424.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58645..58932
                     /gene="hpf"
                     /locus_tag="ACHY03_000134"
     CDS             58645..58932
                     /gene="hpf"
                     /locus_tag="ACHY03_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFS3903425.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59050..59541
                     /gene="ptsN"
                     /locus_tag="ACHY03_000135"
     CDS             59050..59541
                     /gene="ptsN"
                     /locus_tag="ACHY03_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFS3903426.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59587..60441
                     /gene="rapZ"
                     /locus_tag="ACHY03_000136"
     CDS             59587..60441
                     /gene="rapZ"
                     /locus_tag="ACHY03_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFS3903427.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60438..60710
                     /gene="npr"
                     /locus_tag="ACHY03_000137"
     CDS             60438..60710
                     /gene="npr"
                     /locus_tag="ACHY03_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFS3903428.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60923..61555
                     /gene="yrbL"
                     /locus_tag="ACHY03_000138"
     CDS             60923..61555
                     /gene="yrbL"
                     /locus_tag="ACHY03_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFS3903429.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61552..62280)
                     /gene="mtgA"
                     /locus_tag="ACHY03_000139"
     CDS             complement(61552..62280)
                     /gene="mtgA"
                     /locus_tag="ACHY03_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFS3903430.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62277..62930)
                     /gene="elbB"
                     /locus_tag="ACHY03_000140"
     CDS             complement(62277..62930)
                     /gene="elbB"
                     /locus_tag="ACHY03_000140"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFS3903431.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGRECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63160..65496)
                     /gene="arcB"
                     /locus_tag="ACHY03_000141"
     CDS             complement(63160..65496)
                     /gene="arcB"
                     /locus_tag="ACHY03_000141"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFS3903432.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65592..66521)
                     /gene="yhcC"
                     /locus_tag="ACHY03_000142"
     CDS             complement(65592..66521)
                     /gene="yhcC"
                     /locus_tag="ACHY03_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFS3903433.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            67196..71656
                     /gene="gltB"
                     /locus_tag="ACHY03_000143"
     CDS             67196..71656
                     /gene="gltB"
                     /locus_tag="ACHY03_000143"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFS3903434.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71669..73087
                     /gene="gltD"
                     /locus_tag="ACHY03_000144"
     CDS             71669..73087
                     /gene="gltD"
                     /locus_tag="ACHY03_000144"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFS3903435.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73271..74398
                     /gene="yhcG"
                     /locus_tag="ACHY03_000145"
     CDS             73271..74398
                     /gene="yhcG"
                     /locus_tag="ACHY03_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFS3903436.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGVGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRLR"
     gene            complement(74458..74922)
                     /gene="nanQ"
                     /locus_tag="ACHY03_000146"
     CDS             complement(74458..74922)
                     /gene="nanQ"
                     /locus_tag="ACHY03_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFS3903437.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74919..75794)
                     /gene="nanK"
                     /locus_tag="ACHY03_000147"
     CDS             complement(74919..75794)
                     /gene="nanK"
                     /locus_tag="ACHY03_000147"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFS3903438.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75791..76480)
                     /gene="nanE"
                     /locus_tag="ACHY03_000148"
     CDS             complement(75791..76480)
                     /gene="nanE"
                     /locus_tag="ACHY03_000148"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative N-acetylmannosamine-6-phosphate
                     2-epimerase"
                     /protein_id="HFS3903439.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPDIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76528..78018)
                     /gene="nanT"
                     /locus_tag="ACHY03_000149"
     CDS             complement(76528..78018)
                     /gene="nanT"
                     /locus_tag="ACHY03_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFS3903440.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(78127..79020)
                     /gene="nanA"
                     /locus_tag="ACHY03_000150"
     CDS             complement(78127..79020)
                     /gene="nanA"
                     /locus_tag="ACHY03_000150"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFS3903441.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79142..79933)
                     /gene="nanR"
                     /locus_tag="ACHY03_000151"
     CDS             complement(79142..79933)
                     /gene="nanR"
                     /locus_tag="ACHY03_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFS3903442.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        19746 a        21658 c        19882 g        18855 t
ORIGIN      
        1 taaatatgat tatgtacttg ttacaaggat aaagttataa atgaataaag ttacaaaaac
       61 agctattgcg ggcttacttg cgcttttcgc aggtaatgcc gctgcaaccg atggtgaaat
      121 agtttttgat ggcgagattt taaaatccgc ttgtgaaatc aatgactctg ataagaaaat
      181 tgaagttgct cttggtcact ataatgccga acagtttcgt tctgtaggcg atcgtagccc
      241 aaaaattcca ttcactattc ctttagttaa ctgcccagtg actgggtggg agcacgataa
      301 cggcaacgtt gaagcgtctt tccgtttatg gctggaaacg cgggataatg gtaccgtccc
      361 taacttccct aacttggcaa aagtgggctc ttttgctggc acagcagcaa caggtgtggg
      421 tatccgtatt gacgatgcgg aaagcggaaa tcttatgcca ctaaatgcta tgggcaatga
      481 taacacggtt tatcagatcc ctgcggactc cgctggagtt gttaatgttg acctcatcgc
      541 ttactacgta tcaaccgttg aagcatcaga aatcacccca ggggaagcag acgctgtcgt
      601 taacgtaacg ctggattacc gttaatacgt tacggcgtta tctgaccttt cagataacgc
      661 ccttttcctt cctctttctc gttgtatcag gttgaaaaat aactatgtca aaacgaacat
      721 tcgcggtgat aataaccttg ttgtgtagct tctgtatcgg ccaggcgctt gcagggggta
      781 tcgttttaca gcgaacgcga gtgatctatg atgccagccg caaagaggct gcgttacctg
      841 tcgcaaacaa aggcgcagaa acgccttatt tactgcaatc atgggtagat aatatagatg
      901 gtacaagccg tgccccattt attataaccc caccgctatt tcgtcttgaa gctggcgatg
      961 attcatcact gcgaattatt aaaacagctg ataacctgcc tgaaaataaa gagtcgctgt
     1021 tctacattaa tgttcgtgcc attccagcaa agaaaaaatc agatgatgtt aatgctaacg
     1081 agttgacgct ggtatttaaa acacggatca aaatgtttta tcgccccgca cacctgaagg
     1141 gacgggtaag cgatgcgtgg aaatcactgg aatttaaacg tagtgatcat tcactcaata
     1201 tatataaccc aactgaatat tacgtcgtat ttgccggact ggcagtcgat aaagccgatc
     1261 tcacaagcaa aattgaatat atcgcgcccg gagaacataa acagttacca cttccagcat
     1321 ctggcggaaa gaacgtgaaa tgggctgcga tcaatgatta tggcggcagt tccgggacag
     1381 aaactcgtcc actgcaataa caaatataaa aacacaggtc atcagggaat gccacaacga
     1441 caccaccagg gacataaacg cacaccgaaa cagttggcgc tcattatcaa actctgtttg
     1501 ccgatggtgc tcactggcag cggcatgctt tgcactaccg ctaacgccga agagtattat
     1561 ttcgacccca ttatgctgga aaccacaaaa agtggtatgc aaacaaccga tctgtcacgt
     1621 ttttcaaaaa aatacgcaca actaccagga acttatcagg ttgatatctg gctgaataaa
     1681 aagaaggttt cacagaaaaa aattacattt accgccaatg cagagcaact tctgcagcca
     1741 cagtttacgg tagaacaact acgtgagctg ggtattaagg tggatgaaat cccggcgctg
     1801 gctgaaaaag atgacgatag cgtgatcaac tcgcttgaac aaatcattcc cggtacagct
     1861 gctgaatttg atttcaatca tcagcgactt aatttgagca ttccccaaat tgcactgtac
     1921 cgtgatgcaa gaggttacgt ctccccttct cgttgggacg atggtatacc aacgctgttt
     1981 accaactact cgtttacagg ttctgataac cgttaccgcc agggcaatcg tagccaacga
     2041 cagtacctaa atatgcaaaa tggtgccaat tttggcccct ggcgattacg taactattct
     2101 acgtggacac gcaacgatca ggcgtcaagc tggaacacta tcagtagtta tttacaacgt
     2161 gatatcaagg cgttgaagtc tcagttgctt ctgggagaaa gcgccaccag cggcagtatt
     2221 ttttccagct acacctttac tggcgtgcaa ctcgcttccg acgataatat gttgccaaac
     2281 agccagcgcg gatttgcccc aacggtacgc ggtatcgcaa acagtagtgc aatcgtgact
     2341 atcaggcaaa atggttatgt gatctatcaa agcaacgtgc cagcgggtgc ctttgaaatt
     2401 aacgatctct acccctcttc caacagcggc gatttagaag tcacgattga agaaagtgac
     2461 ggtacacaac gtcgctttat ccagccttat tcttcattac ccatgatgca gcgacctggg
     2521 catctaaagt atagcgcgac cgctggacgc tatcgcgctg atgcaaacag tgatagcaag
     2581 gaacccgaat ttgctgaagc cacggcaata tatggtttga ataatacttt tacgctgtat
     2641 ggcggcctgc tcggttctga agattattat gcgctgggga tcggtatcgg cggcacactt
     2701 ggcgcactgg gcgcgttgtc gatggatatc aacagagctg acacccaatt cgataaccag
     2761 cactcttttc atggctatca atggcgtacg cagtacatca aagatatccc ggaaaccaac
     2821 accaatatcg ctgtcagcta ctatcgctat accaacgatg gctattttag ttttgatgaa
     2881 gccaataccc gcaattggga ctataacagt cgccaaaaaa gtgaaattca attcaacatc
     2941 agccagacaa tatttgatgg ggtaagtctg tatgcctccg gttcgcagca agactattgg
     3001 ggcaataacg agaaaaacag gaatatctct gttggggttt ccggccagca atggggaatt
     3061 ggttacagcc tgaattatca atacagccgc tacactgatc aaaataatga ccgcgcactc
     3121 tctttgaatc tcagtattcc gttagaacgc tggttaccgc gtagccgggt ttcctatcag
     3181 atgaccagcc agaaagatcg cccaacccaa catgaaatgc gtcttgatgg ctcactgctg
     3241 gatgatggtc gcctgagcta tagcctggaa caaagtctgg atgacgataa caaccataac
     3301 agtagcctga acgccagtta ccgttcacct tatggaacct tcagtgccgg atacagttac
     3361 ggtaatgaca gcagccaata caattacggc gttaccggcg gcgtggttat ccatcctcat
     3421 ggcgtgacgc tctcgcaata tctgggcaac gcttttgcgc ttatcgatgc taacggggca
     3481 tctggcgtga ggatacaaaa ctatccgggg attgctaccg atccctttgg ctatgcagtg
     3541 gttccttatc tcacaactta tcaggaaaac cgtctctcgg tagatactac gcagctgccc
     3601 gataacgtcg atcttgaaca aacaacacag tttgtggtgc ccaatagagg tgcaatggta
     3661 gcggcgcgtt tcaacgccaa tatcggttat cgcgtacttg ttacagtcag cgatcgcaac
     3721 ggtaaaccgt tgccctttgg cgctcttgcc agcaacgatg agacggggca acaaagtatc
     3781 gtcgatgagg gcggcatact atatctctct gggatatcga gtaaatcaca aagctggact
     3841 gtacgctggg gaaatcaggc tgatcaacaa tgtcagtttg cttttagtac accggattca
     3901 gaaccaacaa cctctgtatt acaaggcacg gcgcagtgcc attaaggata aaaaaatgaa
     3961 aagagcgcct cttatgacag gacttttgtt gatatccaca tcctgcgctt atgcctcctc
     4021 aggagggtgt ggagccgaca gcactagcgg tgcgacaaat tacagcagtg tggttgatga
     4081 tgttacggtg aaccagacag ataacgtgac aggacgggag tttacctctg caacgctaag
     4141 tagcactaac tggcaatacg cctgttcctg ctctgcgggt aaggcagtta aacttgtcta
     4201 tatggtcagc cccgtactta ccaccactgg acatcagaca ggatattaca aactcaatga
     4261 cagcctggat attaaaacca cattacaggc aaacgacatt ccaggactca caaccgacca
     4321 ggttgtctct gttaacaccc gattcacaca gataaaaagc agcaccgtat attctgctgc
     4381 aacccaaacg ggtgtttgcc agggtgacac gtctcgttat ggacccgtta atattggtgc
     4441 gaacaccacc tttaccctgt atgtcaccaa gccatttctc ggctcgatga ccattccgaa
     4501 aacggatatt gccgtcatta aaggcgcgtg ggtcgatgga atgggaagcc cgtctacagg
     4561 tgacttccat gatttagtca agttatcgat acagggaaat ctcaccgccc cacagtcgtg
     4621 caaaattaat cagggcgatg ttattaaggt taattttgga ttcatcaatg gtcagaagtt
     4681 taccacccgc aatgccatgc cagacggttt tactccagta gactttgata tcacttatga
     4741 ctgtggtgat acttcaaaga ttaaaaactc gttgcaaatg cgcatcgacg gtacaactgg
     4801 ggtagtagac cagtacaacc tggtcgccag gcgaagaagt tcagacaatg tgcccgatgt
     4861 cggtattcgt attgaaaatc tcggcggcgg agttgcaaat attccttttc agaacggtat
     4921 ccttcccgtt gatccttccg ggcatggcac cgtcaatatg cgcgcctggc cagttaatct
     4981 ggtcggtggt gagctggaaa caggaaaatt tcagggcaca gccaccatta ccgtcatcgt
     5041 gcggtaaaag aagacgttgc gttttgatta acgcaacgct tcggcactct tacccctgct
     5101 gctccagcgc atacttatat agcgcatttt tcttcacgcc gtgaatttct gctgccagcg
     5161 ccgccgcttt tttcagcggc agttctgcct gcaacagcgc cagcgtgcgc agggcatcgg
     5221 cgggtaagtc ttcttcctgt gctttatgac cttcgacaat cagcaccatt tcgcctttgc
     5281 gacggttttc atcttctttt acccacgcca gcagttcgcc aacgggcgcg ccgtgaatgg
     5341 tttcccaggt tttggtcagc tcacgtgcca gaaccacgta gcgggattcg cctaataccg
     5401 caacgatatc ttccaggcta tctaacagac ggtgggtaga ttcataaaaa atcagcgtgc
     5461 gcggctccgc ttcaatggct tttagcgcat cacggcggcc ttttgatttg gcaggtaaaa
     5521 agccttcgta acagaaacgg tcagagggta aacccgcggc gcttaacgca gtgatagcag
     5581 cacacggtcc gggtagcggc accacacgaa tccccgcttc acggcaggta cgcaccagat
     5641 ggtagccagg atcgttaatt agcggcgttc cggcatcgga aaccagtgca atgttttgcc
     5701 cctcttgcag cttcgccagc agcgtttcgg ctttttgttg ttcgttatgg tcgtgcagcg
     5761 caaacaaccg ggcattaatc ccaaaatgtt gcagcaataa accggtgtga cgagtatcct
     5821 cggcggcaat cagatcaacg gcctgtaata cctctaacgc acgctgggtg atatccgcca
     5881 gattgccgat tggcgtcggt acaatgtaaa gctggccctg agaattatcc gccgattggt
     5941 gttgtttcat tgtgtcgtcc gtattgccga tttaatattg agcattgcgt aaaaaaaata
     6001 tcactggata cattatggta ccctcaacat tttctcgttt gaaagccgcg cgttgtctgc
     6061 ctgttgttct ggcagccctg attttcgccg gttgtggcac ccatactccc gatcagtcca
     6121 ctgcttatat gcagggcacg gcgcaggctg attctgcctt ttatcttcag cagatgcagc
     6181 aaagctctga tgataccagg atcaactggc aattactcgc cattcgtgca ctggtgaaag
     6241 aaggtaaaac cgggcaggcg gttgagttgt ttaaccaact accgcaagaa ctgaatgatt
     6301 ctcagcgtct cgagaaaaca ctgctggcgg tagagattaa actggcgcag aaagattttg
     6361 ctggcgcgca aaacttgctg gcgaaaatta cacccgccga tttagaacaa aaccagcaag
     6421 tccgttactg gcaggcaaaa atcgatgcca gccaggggcg tccttccatt gatttactgc
     6481 gcgcgttaat tgctcaggaa ccgctgcttg gcgcgaaaga aaaacagcag aatattgatg
     6541 ccacctggca ggcgctctcc tccatgactc aggaacaggc gaatacgctg gtgatcaacg
     6601 ccgacgaaaa tattctgcaa ggctggctgg atctgcagcg cgtctggttt gataaccgta
     6661 acgatcccga catgatgaaa gccgggatcg ccgactggga gaaacgttat ccgaacaacc
     6721 cgggcgcgaa aatgctgcca acgcagttgg ttaacgtaaa agcgtttaaa ccagcctcga
     6781 ccaacaaaat cgccctgctg ttgccactga atggccaggc agcggtattt ggtcgcacta
     6841 ttcagcaagg ctttgaagcg gcgaaaaata tcggcactca gccagtggca gctcaggtag
     6901 ctgccgcacc tgccgcagac gtagcagaac aacctcaacc tcaaaccgtg gatggcgttg
     6961 ccagcccggc acaagcctcg gttagcgatc tgaccggtga acagcctgca gcccagccgg
     7021 tgcctgtaag cgccccggcg acaagcaccg cagcggtaag cgcacccgca aatccatccg
     7081 ctgagctgaa aatctacgat acctcatcac aaccacttag ccagatctta agccaggttc
     7141 agcaggatgg cgcgagtatt gtggtcggtc cgttgctgaa aaataacgtt gaagagttgc
     7201 tgaagagcaa cactccgctg aacgtactgg cactgaacca gccggagaat atcgaaaatc
     7261 gcgtcaatat ttgttacttc gcgctttcac cggaagacga agcgcgcgat gcagcgcgtc
     7321 atattcgtga ccagggtaaa caagcgccgc tggtgctgat cccacgcagt tcattgggcg
     7381 atcgcgtagc caatgcgttt gcgcaagagt ggcagaaact gggcggcggc accgttctgc
     7441 aacaaaaatt tggttccacc agcgaattac gcgcgggtgt taacggcggt tctggtatcg
     7501 ctttaacggg taccccgatt actcccagag cgacaaccga ctccggcatg acgaccaaca
     7561 atccaacgct gcaaaccacg ccaaccgatg accagttcac caataatggc ggtcgtgtcg
     7621 atgcggtgta cattgtggca acgccgggtg aaatcgcttt tatcaaaccg atgatcgcca
     7681 tgcgtaacgg tagccagagc ggtgcaacgc tgtacgccag ctcccgcagt gcgcaaggga
     7741 ccgctggccc ggatttccga ctggagatgg aaggcttgca gtacagcgaa atcccgatgc
     7801 tggcaggcgg taatctaccg ttaatgcagc aggcactcag cgcggtgaat aacgattatt
     7861 cactggctcg catgtatgcg atgggcgtcg atgcctggtc gctggcaaat catttctcac
     7921 aaatgcgcca ggttcagggt tttgaaatca acggtaatac cggaagcctg acggctaacc
     7981 cggattgcgt gattaacagg aagttatcat ggctacagta ccaacaaggt caggtagtcc
     8041 ccgccagtta accaccaaac agaccggcga tgcgtgggaa gtacaagcgc gtcgctggct
     8101 ggaaggcaaa ggactgcgat ttgtcgccgc taacgtgaac gagcgtggcg gcgagatcga
     8161 tctgataatg cgtgaaggcc ggaccaccgt ttttgtcgag gtgcgttacc gccgttctgc
     8221 gctttatggc ggcgcggcag ccagtgtgac ccgcagcaaa caacacaaat tattacagac
     8281 tgcccgcttg tggctcgcgc gtcataatgg gagttttgat actgtggatt gccggttcga
     8341 tgtggtagcc ttcaccggga atgaggttga gtggattaag gatgccttta atgaccactc
     8401 ataattaagg tttaaggatt agcgtgcaag aaagaattaa agcttgcttc actgaaagca
     8461 ttcaaactca aattgcggcg gcagaggcgc ttccggatgc catctcccgt gcggccatga
     8521 cgctggttca gtctctgctc aatggcaaca aaatcctctg ttgtggtaat ggaacttccg
     8581 ctgccaatgc acagcatttt gctgccagca tgatcaaccg tttcgaaacg gagcggccca
     8641 gcttacctgc cattgcacta aatactgata atgttgtctt aacggcgatt gccaacgatc
     8701 gcttacatga tgaagtgtat gcaaaacagg tgcgggcgct gggtcatgcg ggagatgtat
     8761 tgttagccat ttccacccgt ggcaacagcc gcgatattgt aaaagcggta gaagctgccg
     8821 ttacgcgtga tatgaccatt gtggcattga ccggctatga cggcggtgaa cttgcaggtt
     8881 tgttagggcc acaggatgtg gagatccgca tcccttcgca tcgcagtgct cgcattcagg
     8941 aaatgcatat gctgacggta aattgcctgt gcgatctgat cgataacacg cttttccctc
     9001 accaggatga ttaaggagaa tacatgaagg cattatcgcc aatcgcagtc cttatttccg
     9061 cgctgctgtt gcaaggttgt gttgccgctg ccgtagtggg tactgctgct gtgggtacca
     9121 aagccgcaac tgacccacgc agtgtcggca cccaggtgga cgatggtacc ctggaagtgc
     9181 gcgtgaacag cgcattgtcg aaagacgaac agattaagaa agaagcgcac attaatgtaa
     9241 cggcctatca gggcaaagtg ctgctggttg ggcagtcacc aaacgctgaa ctttcggctc
     9301 gcgccaaaca aattgctatg ggcgtagacg gtgccaacga agtgtataac gagattcgtc
     9361 agggccagcc gattggtctg ggcgaagcgt ctaacgatac gtggatcacc accaaagtgc
     9421 gttcgcagct cttaaccagc gacctggtga aatcgtccaa cgtgaaagtg accaccgaaa
     9481 acggtgaagt gttcctgatg gggctggtga ctgaacgtga agcgaaagcg gcggcagata
     9541 ttgccagccg ggtgagcggc gtgaagcggg taactacggc gtttacgttt attaaatagc
     9601 agccccttgt aatgcctgat gcgacgcttg ccgcgtctta tcaggcctac aacgatgcaa
     9661 accgtaggtc ggataaggcg tttacgccgc atccgacatg ttttccctca aatcagaata
     9721 acagcgccag cccgccgaca atcacgcctg acactgccac catcgccccc gtcagccata
     9781 aggctttcgc cgggaacgct ttgcgcagca taatcagtga cggcaaactc accgccggga
     9841 gcgtcatcaa caatgccagc gccggagcag ttcccatacc tgccagcatc atcgtttgta
     9901 caatcggaat ttctgctgcc gtgggaatga caaacaagca tcctgccacc gccatcgcca
     9961 ccacccacat caggctgtta tcgacagcac catcggcatg ggggaataac cagacgcgag
    10021 cggcacccaa caccagtact gcaaggatgt aaaccgggat cgtactccag aaaagcgtcc
    10081 atagcgccct gccccagcgg ctaaaaaaac cgccctgtgc ttccggtatg tcaatttcga
    10141 ccggtgcctg cgtttgcggt gtttcacgca cccatttttg caccagcgtc gcaatcagca
    10201 acaccatcac cagcccggcc accagacgaa tcgccgcaaa accccagccg aggacaaacc
    10261 ccataaacac cagcgtcgcc gggtttaaca ccggattgcc catccagaat gccagcgcac
    10321 cgcccatcga cacctgttgg cgacgcattc ccgccgcgac cggagccgca cagcaggtac
    10381 acatcatgcc cggcagcgaa aacagcgttc ccagcagcgt gccgcgaaag cgcgattgcc
    10441 caagcgtacg caacaaccag tcacgcggga tcagcacctg aatcaacgaa ccgaggatca
    10501 cccccagcac tgccgctttc cataccgcga ggaaatagat catcgcgtaa tccaacgccg
    10561 cctgccacgg attagcatcc gcctgcgcaa ggatagattt accgatactg tgggtttcgg
    10621 cagcagtaaa cgctttgccg tagtaaggtt gccatttcac ataccagaga ccggcaatga
    10681 caacgagaaa gaaaagcgcg ggtttccacc actgaatggg cgttgccgcc tgagatgaag
    10741 actgaccagt catagcattc cccaggaata agttatgata attgagcgcg tgaatattac
    10801 gctcactatc aatccttggg aataattatt cagctctttt gcgtaattct gaagagctta
    10861 aaatcgtcac gccttcatgc tcaggccgca tcgcctctgc cagcatgacc cgcgccacgt
    10921 ctctcgcatc aatggatttc cagttaccgg gtaacaaacg gaacagcggc gcaaaaagcg
    10981 tttcgttcat ccgctgtttg ctacgatcgc ccagtaacat cgacgggcga gcaatggtca
    11041 atttcggcca gttctgggcg attaatgctt cttccatctc ccctttgacg cggttataga
    11101 aaaacggcga gtgggcattg gcacccatag cactgaccac cagcatatgc tgcgcgccca
    11161 gtcgccgccc ggttaatgcg gtatccacta ccagcgtgta atcggcatga ataaacgcct
    11221 ctttgctccc cgcttctcgc cgcgtggtgc cgagacaaca aaacacaatg tcgatgggat
    11281 cggtgacctg cgccagcgcg tcagtcagtt gcggatcatg gggattaaac actcctggca
    11341 tatctcccaa cggacgtcgc gtcggcgcgg ctatggcgtt aactttcggt tcgttaatca
    11401 acatccgcag caggtgaccg cccaccagcc ccgttgcgcc tgtaatcagt acctgactca
    11461 tcttcgctcc tttacagaat tgtctacctt gcgctccacg gctcatgcac caggcttaat
    11521 agaccatgag gtaattatcc ccgattgtgg gaaattcgcc tcatccaatg caacaacgcg
    11581 gaggaagcat gagtaagaaa attgccgttt taatcactga tgaatttgaa gattcagaat
    11641 ttacttcacc cgcagacgag ttccgtaaag ccggacacga agtgattacc attgaaaaac
    11701 aagcgggtaa aacggtgaaa ggcaaaaaag gagaagccag cgtgaccatc gataaatcca
    11761 tcgatgaagt gacgccagcg gagtttgatg ccctgttgct accgggcggt tattcaccgg
    11821 attatctgcg tggcgacaac cgttttgtca tctttacccg tgattttgtg aatagcggca
    11881 aacctgtttt cgccatctgt cacggtccgc agctgctgat cagcgccgat gtgattcggg
    11941 ggcgcaaact gaccgcagtt aaaccgatca ttattgatgt taaaaatgcg ggcgcggaat
    12001 tttacgatca ggaagtcgtg gtcgataaag atcagctggt taccagccgg acgccggacg
    12061 atctgccagc gtttaaccgc gaggcgttac gcctgctcgg tgcctgagcc gcgtagccaa
    12121 atcatttttt taccaaagcc cagcgtgttg tcggtgaatt tgatttcatc gagccggatt
    12181 tcccacaccg gggccgacag cattctggca accggaaagc gacgattgta cgccttgcgc
    12241 gcgaggtcgc tttcctcacc ttccagcctg cggatctcac ctttaaactg cacgccgcga
    12301 attaacgcta ccgttttcgg ctgaccgttt accgttccgg caaccgcagc ctgcggccca
    12361 ctcatctgcg cgtggcgcgt tttttcttcc gtcaaaatgt agaaggcgac tttctgcgca
    12421 tcaaacagat aaaaagcatt agcgcaccag agttcccctt cctgctgcac acaccaggtg
    12481 acaacatgtt gttttgccaa ccaacggctg atggtgatga gtgtttccat tgctgttctc
    12541 ttttatactg tgggcctgac cttaacatgc gacgcagacg atgacaccct ggtttcttta
    12601 cttgatccgt accgccgaca ataagcttta taccgggatc accacggatg tcgaacgccg
    12661 ctatcagcag caccaaagcg gcaaaggggc gaaagcactg cgcgggaaag gtgaactgac
    12721 gctggcattt tccgcgccag tgggcgatcg ttcgctggcg ttacgggcgg aatatcgcgt
    12781 taagcaactg accaaacggc aaaaagagca tctggtagcg gaaggcgcag ggtttgcaga
    12841 gctgttaagc agtctgcaaa ccccggagat taaaagcgat tgaaatgctc gtgatactca
    12901 accagaccgg ttacgccatt cagcgcgtca tccgctaaac gatgtacctg gaaggcgctt
    12961 tcagtgcccg gccagcggca acgcagatcg tgatgcgccg ccagttcaaa gccgaaacga
    13021 ctgtacagcg ccggatcgcc cagcgtcacc actgcggcat agccgaactc attaagcgaa
    13081 tcgagtcctt catagaccag ttggcgtgcc agcccctgtc cacggtattt ttcatctacc
    13141 gccagcggtg ccatgccgac ccattgcagg tcttcgccct gcacatcaac cgggctaaat
    13201 gccacatagc caatgacctg accttcgtca tctgtcgcca ccagccccag cgtcagaaag
    13261 ccatcttcac gcagatcgtg aaccagcttc gcttccgcat cactttcgaa tgaacgacgc
    13321 agcagggcat caatacccgg cgcatcaatg ggaatttcta ctcgaattag catggttcac
    13381 ctaccgatgt ctgtttggtt tcaggcgcgg ttttcattcc cgcctcaaca aaatccgcca
    13441 gttgcagcag catcatgcgt aaggctttcg gcatttgctc cagttcaatg gcgtccatca
    13501 ggtttttcac atacagcccc agctccgtat cgccttcaat caccagccga cgctggaaga
    13561 agagcgtatc cggatcctgt ttacgcgccg caatcatcag cagatcgctg gcgtcggcac
    13621 taaaactcac atcagcttgc gcgttctggc tgaccaccag tttgccattc accaccgagg
    13681 taaaccattg caggtcaata tcacgcacat gaatacttaa ccagcggcct tcaagaaact
    13741 ccagctcgcc atcatccagc gcctgacgga attgccagct taagacctgc tcaagaacct
    13801 ggcgttttag cgcaaatggc gtcagtttta ccggtacact caacagagat ggccccaaat
    13861 gcacaatacg ggaacgcagt ttatccaaca cgagttttac tccctgtttc aacaatcatc
    13921 ctattttgcc atatcagaaa aataacatag cggtataaat caacaattcc atatgaaatt
    13981 gctgctacca ccaatacaac tttaactgcc ttaaatcaaa aattgtcgca gcaaggttaa
    14041 ctaaaatccc agttcgttaa catttttgcg ttttgatagc gcaaccttca ggaaaaatta
    14101 tggagctgct ctgccctgcc ggaaatctcc cggcgcttaa ggcggccatc gaaaacggcg
    14161 cagatgctgt ttatatcggg ctaaaagatg ataccaatgc ccgtcacttc gccggcctta
    14221 actttaccga gaaaaaattg caggaagcgg tgagttttgt ccatcaacat cgccgcaaac
    14281 ttcacatcgc gattaacact tttgcgcatc cggacggtta cgcccgttgg cagcgtgccg
    14341 tggatatggc ggcgcagctg ggtgccgacg cgctgatcct cgccgacctc gccatgctag
    14401 agtacgccgc cgagcgttac ccgcatattg agcgtcatgt gtcagtgcag gcttcggcga
    14461 ccaatgaaga ggcgattaac ttttatcatc gccattttga cgttgctcgc gtggtgctgc
    14521 cgcgcgtgtt gtcgattcat caggtgaaac aactggcacg ggtcacacct gtaccactgg
    14581 aagtctttgc ttttggcagc ctgtgcatta tgtcggaagg ccgttgctat ctgtcgtcgt
    14641 atctgacggg tgagtcgccc aacaccgtag gcgcgtgttc tccggcccgt ttcgtgcgct
    14701 ggcaacaaac gccgcagggg ctggaatccc gcctgaacga agtgctgatc gaccgttatc
    14761 aggacggcga aaacgcaggt tatccgacgt tgtgtaaagg gcgttatctg gtggacggcg
    14821 agcgctatca cgcgctggaa gaaccaacca gtctcaatac cctggaactg ctgccggagt
    14881 taatggcgtc gaatatcgct tcggtgaaaa ttgaaggccg ccagcgtagc ccggcgtatg
    14941 tcagccaggt ggcgaaagtc tggcgtcagg ctatcgaccg ttgtaaggcc gatccgcaaa
    15001 acttcgtacc gcaaagcgcg tggatggaga cgctcgggtc gatgtccgaa ggcacgcaaa
    15061 ccacccttgg cgcgtatcac cgtaaatggc aatgagaaga gcaatgaaat attccttagg
    15121 gccagtgctg tggtactggc caaaagagac gctggaagat ttttatcagc aggccgccac
    15181 cagcagcgcc gacgtgattt atcttggcga agcggtatgc agcaagcgcc gggcaaccaa
    15241 agttggcgac tggctggaga tggcaaaatc gctcgccgga agtggtaagc agatagtgct
    15301 ctccacgctg gcgctggtgc aggcatcatc tgaactgggc gaactgaaac gctatgttga
    15361 gaacggtgag tttctgattg aagccagcga tctcggcgtg gtgaatatgt gcgctgaacg
    15421 caaactgccg ttcgtcgccg gacacgcgct gaactgctat aacgcggtga cgctgaaaat
    15481 attgctcaaa cagggcatga tgcgctggtg tatgccggtg gagctttccc gcgactggct
    15541 ggtgaatctg cttaatcagt gcgatgagct gggcattcgc aaccagtttg aagtggaagt
    15601 cctgagctac ggtcatctac cgctggccta ctccgcccgc tgctttaccg cgcgttcgga
    15661 agaccgcccg aaagatgagt gtgaaacctg ctgcattaag tatccgaacg ggcgcaacgt
    15721 gctgtcgcag gaaaaccaac aagtgtttgt actcaatggc attcagacca tgagcggcta
    15781 cgtttacaac ctcggtaacg agctggcatc catgcagggg ctggtcgatc tggtccgcct
    15841 gtcaccgcag ggtactgaca ctttcgcgat gctcgacgcc ttccgcgcta atgaaaatgg
    15901 cgctgcacca ctgccgttga cggcgaacag cgactgtaac ggctactggc ggcggctggc
    15961 aggactggag ctgcaagcct aagtaaatag ctcactttgt taacaacttt aactactctt
    16021 taatgcagta ttaaagatta atcggtaaca aagtgagctg ttatgactga taaaaccatt
    16081 gcgttttcgc tactcgatct ggcccccatc cccgaaggtt cttcagcacg agaagcattc
    16141 tcccactctc tcgatatcgc ccgtctggct gaaaagcgcg gctatcatcg ctactggctg
    16201 gcggaacacc acaatatgac cggcattgcc agtgctgcaa catcggtatt gattggctat
    16261 ctggcggcaa ataccaccac gctgcatctg ggttctggcg gcgtgatgtt accgaaccat
    16321 tcgccattgg tgattgccga gcagttcggc acgcttaata cgctctatcc ggggcgaatc
    16381 gatttagggc tgggacgtgc tccaggtagc gatcagcgga caatgatggc gctacgtcgt
    16441 catatgagcg gcgatgttga taatttccct cgcgatgtcg cggagctggt ggactggttt
    16501 gacgcccgcg atcccaatcc gcatgtgcgc ccggttccgg gctatggcga gaaaatcccg
    16561 gtgtggttgt taggctccag cctttacagc gcgcaactgg cggcgcagct tggtctgccg
    16621 tttgcgtttg cctcacactt cgcgccggat atgttgttcc aggcgctgca tctttatcgc
    16681 agcaacttca aaccgtcggc acgactggaa aaaccatacg cgatggtgtg catcaatatt
    16741 atcgctgccg acagcaaccg cgacgctgaa tttctgttta cctcaatgca gcaagccttt
    16801 gtgaagctgc gccgtggcga aaccgggcaa ctgccgccgc cgattcaaaa tatggatcag
    16861 ttctggtcac cgtctgagca gtatggcgtg caacaggcgc tgagtatgtc gctggtgggc
    16921 gataaagcga aagtgcgtca tggtttgcag tcgatcctgc gcgaaaccga cgccgatgag
    16981 attatggtta acgggcagat tttcgaccac caggcgcgcc tgcattcgtt tgagctggcg
    17041 atggatgtta aggaagagtt gttgggatag tgtgtcttaa cgcgggaagc cttatccgag
    17101 ctggcaacgc tgtcctacat agacctgata agcgaagcgc atcaggcatt gtgtaggcag
    17161 cagaaatgtc ggataaggca tcgctgatta ctgatacacc ggcagtaaat taaagctcga
    17221 taaaatatgc accagtgcgt tgccgacgcc aaacaccaga atcagcgcaa tcatcggctt
    17281 gccaccccag acgcggaatt tcgggctgcc aaagcgttta cgcgatgcac gggctaacag
    17341 cgccggaaca attgccgccc agatggtagc cgctaaacca gcataaccaa tggcgtacag
    17401 gaatccgttc gggaacaaca gcccccccac aactggcggg gcaaaggtca gcaatgccgt
    17461 tttcaagcgc cccagggctg agtcatcgaa accaaacaga tctgccagat agtcaaacaa
    17521 acccagtgtt acgccaagga acgaactcgc taccgcaaag tttgagaaca cgaccagcag
    17581 aaggtccagg ctacggctgt tcagcacgcc gcttaatgct tgcaccagca catcaatgtt
    17641 accgcccttc tcggcgatgc cgataaactc cggacgaggg atgttgccca ttgtcgccag
    17701 caaccagatg gtatacagcg ccagtgccat cagcgtaccg tacaccaggc atttcacgat
    17761 ggttttcgga tctttgccgt aatacttcat caggcttggc acgttaccgt gataaccaaa
    17821 cgatgccaga cagaacggca gggtcatcaa cagatacggt gcataagacg cattgctttc
    17881 ggcgacgttg aacaatgtcg caggctgcac atgccccagc aggctaccaa aggtgaggaa
    17941 gaaggtaatg actttcgccc ccagcacaat cgctgtcatg cgactgacgg ctttagtgct
    18001 caaccacacc acaaacgcta ccagcaatgc aaaaccaaaa cccgccgccc gtgccgggac
    18061 gtttagtgac atctctgcga aggtgtgatg cagaatcgaa ccactggcag aaatataggc
    18121 ataggtcagg atatagagca caaaggcaat ggaaatgccg ttgaccacgt tccagccttt
    18181 gcccagcaaa tctttggtga tggtgtcaaa actcgaaccg attctgtaat tcaggttagc
    18241 ttccagaatc atcaagccgg aatgcagcat acagaaccag gtaaagatca gcgccgccat
    18301 tgaccagaaa aaccacgccc cggacatgac cactggcaga gaaaacatcc ctgcgccaat
    18361 aatggtgccg ccgataatca ccacgccgcc aagcagcgac ggtgacgttt gggtggtggt
    18421 tagtgttgcc atgagggcct ctctccagtg aaaaatagtg cgactgcgtt gttatgtatt
    18481 gcactgtacc agtacacgag tacaaaagac agaaaaaaag ccccgatggt tcaaatcggg
    18541 gctgtatata ttattttaca gactgtgttc gctgttcagc gatgattacg catcaccacc
    18601 gaaacgacga cgaccggtag agtcatcacg acgcggagcg cggccttcac gacgttcgcc
    18661 gctaaaacga cgaccatcac cacgaccacc ttcacggcgt tcaccgctga agttacgacc
    18721 accttcacga cgctcgccac tgaaaccacg accaccgcca cgacgctctc cgccagtatg
    18781 cggctgtgca tcgcccagta attgcatatt catcggcttg ttcagaatgc gagtacgtgt
    18841 aaagtgttgc agcacttcac ccggcatacc tttcggcagt tcgatggtgg agtgagaagc
    18901 aaacagctta atgttaccga tgtaacggct gctgatgtcg ccttcgttag caatcgcacc
    18961 aacgatatga cgaacttcaa caccatcatc gcggcccact tcaatacggt acaactgcat
    19021 gtcaccaaca tcacgacgtt cacgacgcgg acgatcttca cggtcgccac gcgggccacg
    19081 gtcgttacga tcgcgcggac cacggtcatc acggtcacgg aattcacgtt tcggacgcat
    19141 cggcgcatct ggcggaacaa tcagagtacg ttcaccctgt gccattttca gcagtgcggc
    19201 ggccagagtt tcgagatcca gctcttcacc ttctgcagtc ggctgaattt tgctcagcag
    19261 tgcgcggtat tgatccagat cgctgctttc cagctgctgc tgtactttag cggcgaattt
    19321 ttccagacgg cgtttgccca gcagttctgc gttcggcagt tctacttccg gaatagtcag
    19381 cttcatagta cgttcaatgt tgcgcagcag acgacgctcg cggttctcaa cgaacagcag
    19441 cgcgcggcca gcacgacccg cacgaccggt acgaccgata cggtgaacgt aagactcaga
    19501 atccatcggg atatcgtagt taaccaccag gctgatacgc tcaacgtcca ggccacgggc
    19561 cgcaacgtcg gtcgcaatca ggatgtccag acgaccatct ttcaggcgtt ccagtgtctg
    19621 ctcacgcagc gcctggttca tgtcaccgtt cagcgcggcg ctgttgtagc cgttacgctc
    19681 aagagcttca gccacttcca gagtcgcatt tttggtacga acgaagataa tcgccgcatc
    19741 aaaatcttcc gcttccagga aacgtaccag tgcttcgttt ttacgcatac cccagacagt
    19801 ccagtagctc tggctgatgt caggacgggt agtcacgctg gactgaatgc gcacttcctg
    19861 cggctctttc ataaagcggc gggtaatgcg acgaatcgct tccggcatgg ttgcagagaa
    19921 cagagcggtc tgatgacctt ccgggatctg cgccataatg gtttcaacgt cttcgatgaa
    19981 gcccatgcgc agcatttcgt cagcttcatc cagaaccaga ccgctcagtt tagagaggtc
    20041 cagagtgcca cgtttcaggt ggtccagcag acggcccgga gtaccgacaa cgatctgcgg
    20101 cccctgacgc agggcgcgta attgcacgtc ataacgctgg ccgccgtaca gggcaaccac
    20161 atttacgccg cgcatgtgtt tagagaaatc cgtcattgct tcagcaacct gtaccgccag
    20221 ttcgcgggtc ggtgccagca ccagaatctg tggtgctttc agctcaggat caagattctg
    20281 cagcagaggt aaagagaatg ctgcagtttt tccgctcccc gtctgggcca tacccagaac
    20341 gtcgcggcca ttcagcaaat gtggaataca ctctgcctga attggagatg gtttttcgta
    20401 acccagatcg ttaagggctt caaggatagg agccttcagg cccagatctg caaaagtggt
    20461 ttcgaattca gccatgtagt acgtgtgcct caaaattaat ggcggccagt ctacataact
    20521 catcatgaaa ttgatcagca attttcattg aaaagtgtga accggctcaa agtaggtgta
    20581 ttaacgaaca acaacgccct cacccgttaa ggtgatggca atcaaaaaag attacgggct
    20641 gatgtgtacg tcagctattg ctggtccgat tctgccaggt catcttggtc ctggcccagg
    20701 agcgataatt ccaacaatgc gtatcggtgc tcaacaaagt tatgaacgtt gttggcaacc
    20761 gccagtttga acagtgccgt ggcgctgtcc aaatccccca gacttaggta gtacttacct
    20821 aaatagaagt tggtttcact gagatgctca gcgagcgagg tgttatccgt tgcgtccgcc
    20881 ttgagccttt ccattaacgt ttgttcgcta atgttgccca ggtagaactc gacaatgttc
    20941 catccccact gttccttatc cgatttttcg aagtgctgtt tcaacacttc tttagcctgc
    21001 ttctcatcga gcttctgctc ggcgagataa agccacagac tacggaaagg atcattggga
    21061 tcgtcttgat aaaacgccag cagatcatct tgcgctaact tgtcacgacc gccgtaatat
    21121 aatgcgatcc cgcgattcaa gtgcgcgtag ttgtaagttg gatcaagctc aagtacagaa
    21181 tcaaacgctt cataggcagc atcaaaattg cctgcctgcg ttaaatatat gcctaagtaa
    21241 ttgaatactt caggcatatc cggtcggatt gccagcgctt gcgaaaaatc gttacgcgct
    21301 aatgccctca gaccgagact atcatacaac actccgcgct catataaaag ctgtgcgcgt
    21361 tcgtcatcgg ttaaagcccg actggcaagg atttgttcca tacgtgccag aatcacttcc
    21421 tgctgtaaag tcggttgcaa tggtaccgcg aggacttcac ttttacgcca ggaagtatta
    21481 ctgcatcctg caagcgtaag tgctgtcgca acgaaacacc agcgcaaaaa aggcttcatt
    21541 tcccactccc gaagaccacg gttgaatgaa cgtcctgttc ccggttgcta acaaggcgtc
    21601 ctgcccggtt aaaagccccc cgccgcagcg gagggcaaat ggcaacctta ctcgccctgt
    21661 tcagcagccg gagcttccgg tgctgcagca ggttgagact gctcagtcgc ttctttaatg
    21721 ctcagacgga tacggccctg gcgatcaact tccagaactt tcaccggtac ttcctgaccc
    21781 atctgcaggt aatcggtcac tttctcaacg cgtttgtcag cgatttgaga gatgtgtacc
    21841 agaccttctt taccgccgcc gatggcaaca aatgcgccga agtcaacgat acgggtcact
    21901 ttaccagtgt agacgcggcc cacttcgatt tctgcagtga tctcttcgat acgacgaata
    21961 gcgtgtttcg ctttctcgcc gtcggtcgct gcgatcttca cagtaccgtc atcttcgatt
    22021 tcgatggtgg tgccagtttc ttcggtcagg gcacggatca cagagccgcc tttaccgata
    22081 acgtctttga tcttgtccgg gttgatcttg atggtatgga tacgcggtgc gaactcagag
    22141 atatcgccac gcggcgcgtt gatcgcctgt tccattacgc ccaggatatg cagacgcgca
    22201 cctttagcct ggttcagcgc aacctgcatg atctctttgg tgataccttc aattttgata
    22261 tccatctgca atgcagagat accgtcgcgg gaacccgcaa ctttgaagtc catatcgccc
    22321 aggtgatctt cgtcgcccaa aatgtcagac agcacaacgt agttgtcgcc ttctttcacc
    22381 agacccattg cgatacccgc aacggcagct ttgattggca cacctgcgtc catcagcgcc
    22441 agagacgcgc cgcacacgga agccatagaa gaagaaccgt tggattcggt gatttcagac
    22501 actacacgta cggtgtacgg gaatttgtcc atatccggca tgactgccag cacgccgcgc
    22561 ttcgccagac gaccgtgacc aatttcacga cgcttcggag aaccgaccat gccggtttcg
    22621 cctacggagt acggagggaa gttgtagtgg aacaggaagg tatcggtacg ttcgcccatc
    22681 agttcatcaa gaacctgcgc gtcacgagca gtacccagcg ttgcggtaac cagcgcctgc
    22741 gtttcaccac gggtgaacag cgcagaaccg tgagtacgcg gcagcacgcc agtacgcaca
    22801 tccagaccac ggatcatatc tttttcacga ccatcgatac gcggttcgcc tgccagtacg
    22861 cggctacgaa caacgttttt ctcgatcgcg tgcagaattt cacccagttc gttttcgtcc
    22921 agggtttcgt cttcagcaag cagcgttgcg atggtttcag atttgatgac atcaacctgc
    22981 gcataacgct cttgtttgtc ggtgatgcgg taagcatcgc tcagacgagc ttcagccagt
    23041 gcagcaacgc gcgcgttcag cgcttcgttt accggctccg gctgccagtc ccaacgcggt
    23101 ttaccggctt ctttcaccag ttcattgatg ttctgaataa caacctgctg ttgttcatga
    23161 ccgaacacca ctgcgcccag catctggtct tcgctcaaca gttcagcttc agattcaacc
    23221 atcagcacgg cagcttcagt accggcaacc accaggtcca gcttgctctc tttcagctcg
    23281 tcctgagtcg ggttcagtac gtactggtca ttgatgtaac ctacgcgggc agcaccaatc
    23341 ggaccattga acggaatacc agacagagac agtgctgcgg aagcaccaat catcgcgacg
    23401 atatccgggt taacctgcgg gttaacagaa accacggtgg cgataacctg aacttcgttg
    23461 acgaaacctt ccgggaacag cgggcgaatc gggcggtcaa tcagacgcgc gatcagcgtt
    23521 tcgccttcgc ttgggcggcc ttcacgacgg aagaagctac ccgggatacg accagcagcg
    23581 taggtacgct cctgatagtt aacggtcagt gggaagaagt cctgacctgg tttggctttt
    23641 ttctggccaa caacggtaac gaataccgcg gtgtcatcca tgctaaccat aacagcggca
    23701 gtagcctgac gagccatcat gccggtttcc agagtcacgg tgtgttggcc gtactggaat
    23761 ttacgaacga tcggattaag caatgtaata tcctttctct ttcttagaca gtaccttacg
    23821 gcactggtgt taatacccga tcttctgcgc atcctcgcga ctaatgacaa ccctaaccca
    23881 gcccaatgtg ggtaaagcct ctcattagcc gcgcgaacct ctgcaacgga agatcattca
    23941 tagcaacaat acattagttt ccagtgaatt gctgccgtca gcttgaaaaa aggggccact
    24001 caggccccct tttctgaaac tcgcaagaat tagcgacgca gacccaggcg ctcgatgagc
    24061 tgggtgtaac gtgctacgtc tttacgtttc aggtagtcga gcagtttacg acgctgagaa
    24121 accatgcgca gcagaccacg acggctgtgg tgatcttttt tgtgctctgc aaagtggccc
    24181 tgcaggtgat tgatctgtgc agtcagcagt gctacctgaa cttcggtaga accggtgtcg
    24241 tttgcgtcac gaccaaactc agaaacgatt ttagctgttg cttcagtact tagagacatt
    24301 ttaaaactcc aaagtatata gaatgaaagg acgccgatct ctaattcagc gatcccagtg
    24361 tacgttacgc aaagtgttaa acaatttacg cgacgttaag cggcagtatt ctactcgtag
    24421 cgacctgtta tcgcaagacg gttaacatta cgccgggtat tcaaccacca ggcgacgagg
    24481 cgcaacgcgg ccttcatcgt caatttcgcc cataccgata aatttgccgt tctcaccttc
    24541 cgtgacgcga accagtcctt ccagcggtgc accagatgta cgaaccgggt taccattttt
    24601 gaagtaaaca gaagacgtta acggaagatt caccaccgga tagtccgaag ctggactgtc
    24661 cattggcatc agtaatggat caagtaactc cgcagccgga atatcctgct gttcagcttg
    24721 ctcaacaagt tcacgcaggt gctccagggt caccatccgt tcaaccggat atttacttac
    24781 cgccagacga cgcaggtaaa taacatgcgc gccacaaccg agtttttcac ccaggtcatc
    24841 aatgatggtg cggatataag tgccttttga gcagtgaatt tccagctcca gctcattgcc
    24901 ttcatggcga ataaacagca attcataaac ggtaatcgga cgcgcttcac gcggaacttc
    24961 aatgccctga cgcgcatatt cgtacagttt tttgccctgg tatttcagtg ctgaatacat
    25021 cgatgggatc tgttcgatat cgccacggaa agtatccagt gccgccgcca gttgttctgc
    25081 actaaaggtt accggacgct cttcaacgat ttgtccgtcg gcgtcagaag tatcggtacg
    25141 ctgtccaaga cgcgcaatga cccgatagcg tttgtcggag tccagcagat actgggaaaa
    25201 cttcgtcgct tccccgaggc aaatcggcaa catgccggtc gccagcgggt ccagcgcacc
    25261 ggtatgcccg gcacggttgg cgttataaat ccgtttcact ttttgcagcg catcgttgct
    25321 ggacataccc tgaggtttat ccagcaacaa aacgccgtta atgtcgcgac cgcgacgacg
    25381 aggacgactc attagtcctc cttgctgtcg tccgggttaa cacgacgttc ttcgtcatgt
    25441 ttgaccacgc tggtcaccag gtttgacatg cgcatccctt caaccagaga gttgtcgtag
    25501 aagaaggtca gttccggcac gatacgcagg cgcatcgctt tccccagcag gctgcggatg
    25561 aaaccagaag cttcttgcaa cgctttgatg cccgctttaa ccgcgtcttc atctttgtcg
    25621 ttgaggaacg tcacatatac tttggcatac gccaggtcgc gagacatttc gacaccggaa
    25681 acggtggtca tcatgcccag acgaggatct ttaatttcac gctgcaggat gagagcgatc
    25741 tctttttgca tttcctgcgc tacgcgctgc gggcgaccaa attctttcgc cataataaat
    25801 tctcctgaca aaaaaggggc tgttagcccc tttttaaaaa ttaatttcag gtggaagggc
    25861 tgttcacgtt ggcctgataa gacgcgccag cgtcgcatca ggcaatccat gccggatgcg
    25921 gcgtaaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggatct
    25981 cgatgatttc gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc
    26041 cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg
    26101 actccagctc gccttcatag ataaccacgt tgtcacgcag aacgcggatc gggttatgac
    26161 gtttaaccac accttcggta accatacagc ctgcgatggc accaaatttc ggtgatttga
    26221 acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac
    26281 cgctcatcgc cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat
    26341 ccaggctttc agcctcaatc actttacgcg cagatgcatc ggcacgaacg ttaaagccaa
    26401 ccagaatagc gttggatgct gccgccaggg tggcgtcggt ttcggtgata ccacctacgc
    26461 cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagtaag gagtcggaga
    26521 tcgcttctac agaaccctgt acgtctgcct tgaggactat attcacttcg tgaacttcgc
    26581 cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa
    26641 cttcgcggaa tttaccctga cgatagagtg caacttcacg cgctttcttc tcgtcacgca
    26701 caacggtaac ttcatcaccc gcagccggaa caccggacaa gccgaggatt tccaccggaa
    26761 tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac
    26821 cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa
    26881 cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca
    26941 tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca
    27001 gttcatcgat accggtaccc gctttcgcag atacgtgtac gaactggctt tcaccgcccc
    27061 actcttccgg caggatgccg tactgggaga gttcattctt aacgcgatcc ggatcggctt
    27121 ctggtttatc gatcttgttc actgcaacca ccaccggtac ctgcgccgct ttcgcgtgct
    27181 ggattgcttc gatggtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga
    27241 tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa cgcggcgtgc cccggggtgt
    27301 ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgggtaa
    27361 tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt
    27421 taccgtggtc aacgtgaccc atgatggtca caaccggagc gcgcggttca gccgcagccc
    27481 ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt
    27541 tgtggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg
    27601 ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct
    27661 tgttcgccag ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct
    27721 gagcaggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg cccttaccgc
    27781 cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt
    27841 tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat
    27901 cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat
    27961 cctcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt
    28021 cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttcg gctttacgct
    28081 tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat
    28141 cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag
    28201 cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag
    28261 ccgattcttc tgcctcacga cgggcttgct cttccgcttc acgctgcgct tgctcttccg
    28321 ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa ggtgcgtttc ttgcggactt
    28381 cgatttgtac cgatttgctt tttccaccgg taccaggaat gttaagggtg ctgcgtgttt
    28441 tacgttgcag cgtcaatttg tccgggcctg aatttttctg attcaggtgg tcaatcaaag
    28501 tctgtttctc ttgtgcagac acagagtcgt cagcagactt ccggatacct gcatcagcaa
    28561 attgctgtac caggcgttcc acggaggtct gtcgctctgc ggccagcgtt ttaatcgtta
    28621 catctgtcat gctgttcctt cctgctacag tttattacgc ttcgtcaccg aaccagcaaa
    28681 tattacgggc agccataatc agtgctccgg ctttttcgtc ggtcaaccct tcgatatcag
    28741 ccagatcatc aatgccctgt tcggcgagat cttccagcgt acaaacgcca cgggcggcca
    28801 gtttgaatgc caaatcacga tctacccctt caaggttcag cagatcgtca gccggtttgt
    28861 tatcaccgag gctttcttcc tgggcctgtg caatggtggc cagtgcattt ttagcacgct
    28921 cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat ttccaacagc tctttcatcg
    28981 gcacataggc cagttcttcc agcgtcgaga aaccttcttc taccagaaca gtcgcgaagt
    29041 cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc tgcgtgcgct tccgcctgat
    29101 gcttagcctg caggtcgtca acggtcatta cgttgagttc ccaaccgctc agctgcgaag
    29161 ccagacgcac gttctgaccg ttacggccaa tcgcctgtgc caggttaccg gcttcaacgg
    29221 cgatatccat ggtgtgttta tcttcatcca ccacgataga agcaacgtct gccggtgcca
    29281 ttgcgttaat cacgaactgc gccgggttat catcccacag gacgatatcg atacgctcgc
    29341 cgcccagttc agtagaaacc gcctgaacac gcgcgccacg catacctacg caagcaccta
    29401 ccggatcgat acgtttatcg ttggttttca ccgcgatttt cgcacgagaa cccgggtcgc
    29461 gagccgctgc tttaatttca atcacttctt cgccgatttc tggcacttca atacggaaca
    29521 gttcgatcag catttccggc ttggaacgag tgacgaacag ctgcgcgcca cgcgcttccg
    29581 ggcgaacgga atagagcacg ccacgaacgc ggtcgccagg gcggaagttt tcacgcggca
    29641 gcatatcttc gcgcaggatc acggcttcag cgttgttgcc cagatccaga gagatgttgt
    29701 cgcggtttac ttttttcacc acgccggtga tgatttcacc ttcgtgttca cggaactgat
    29761 caaccaccat cgcacgttcg gcttcacgca ctttctgcac gataacctgt tttgccgtct
    29821 gggtggtgat acggtcaaag gtaacagact caatctgatc ttcaacgtaa tcgcccaggt
    29881 tcaggctttc atcttcataa cgtgcggctt caagggtgat ttccttggtc ggctgggtga
    29941 cttcatcaac aactaaccag cgacggaagg tgtcaaaatc accgcttttg cgatcgatct
    30001 gtacgcgaac gtcgatctct tgttcatatt ttttctttgt tgctgtcgcc agcgcgcttt
    30061 ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc attggatacg gcttcaacta
    30121 cagccaaaat ttctttgttc atcgcgggct tttcacctca tccagactat taaaagtggg
    30181 gaaccaggtt cgccttctgg atattactca gcgcgaacac ttcatcttta ccttcgacgg
    30241 taactgtgat catttcaccg tctaccgctt tgataacacc ctgccattta cgacggtttt
    30301 gtaccgccat acggagaagc agagtcacct cttctccgac aaaacgggcg tagtgttcag
    30361 ccgtgaacag tgggcgatcg agacccggtg aggagacttc caggttataa gcaacggtga
    30421 tgggatcttc aacatccagc acagcactta cctggtggct cacatcagca caatcatcaa
    30481 cattgatgcc atcttcacta tcaatataga tgcgcagtgt ggatgtgcga ccgcgaataa
    30541 attcgatgcc aaccagttca aaacccaggg cctcaactgg cgcagtaatc atctctgtta
    30601 atttttgctc taatgtggac aagcccaccc ccaagacata aaaaaagggc ctaaagccca
    30661 gttattctgt agtcagataa caaaaaaccc cgataaatcg gggctttata taactgaacc
    30721 ctataaccgc aactgcggtc tggagcactt tccagaagga ttttttcaaa tcccactacg
    30781 aaggccgaag tcttcacagt atatttgaaa aaggactcta agggaaagtg gttgcggggg
    30841 ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg ctgctccacc
    30901 ccgcgcctga aacgtggcaa attctactcg ttttgggtaa aaaatgcaaa tactgctggg
    30961 atttggtgta ccgagacggg acgtaaaatc tgcaggcatt atagtgatcc atgccacatt
    31021 ttgtcaacgt ttattgctaa tcacgtgaat gaatatccag ttcactttca tttgttgaat
    31081 acttttgcct tctcctgatc ccccttaagc gcattatttt acaaaaaaca cactaaactc
    31141 ttcctgtctc tgataaaaga tgattaaatg aaaactcatt tattttgcat aaaaattcag
    31201 tgagagcgga aatccaggct catcatcagt taattaagca gggtgttatt ttatgacgac
    31261 gattctcaag catctcccgg taggtcaacg tattggtatc gctttttccg gcggtctgga
    31321 caccagtgcc gcactgctgt ggatgcgaca aaagggagcg gttccttatg catatactgc
    31381 aaacctgggc cagccagacg aagaggatta tgatgcgatc cctcgtcgtg ccatggaata
    31441 cggcgcggag aacgcacgtc tgatcgactg ccgcaaacaa ctggttgccg aaggtattgc
    31501 cgctattcag tgtggcgcat ttcataacac caccggtggc ctgacctatt tcaacacgac
    31561 gccgctgggc cgcgccgtga ctggtaccat gctggttgct gcgatgaaag aagatggcgt
    31621 gaatatctgg ggtgacggca gcacctataa aggaaacgat atcgaacgtt tctatcgtta
    31681 cggtctgctg accaatgctg aactgcagat ttacaaaccg tggcttgata ctgactttat
    31741 tgatgaactg ggcggtcgtc atgaaatgtc tgaatttatg attgcctgcg gtttcgacta
    31801 caaaatgtct gtcgaaaaag cttactccac ggactccaac atgcttggtg caacgcatga
    31861 agcgaaggat ctggaatacc tcaactccag cgtcaaaatc gtcaacccga ttatgggcgt
    31921 gaaattctgg gatgagagcg tgaagatccc ggcagaagaa gtcacagtac gctttgagca
    31981 aggtcatccg gtggcgttga acggtaaaac ctttagcgac gatgtagaaa tgatgctgga
    32041 agctaaccgc atcggcggtc gtcacggcct gggcatgagc gaccagattg aaaaccgtat
    32101 catcgaagcg aaaagccgtg gtatttacga agctccgggg atggcactgc tgcacattgc
    32161 gtatgaacgc ctgttgaccg gtattcacaa cgaagacacc attgagcagt atcacgcgca
    32221 tggtcgtcag ttgggccgtc tgctgtacca ggggcgttgg tttgactccc aggcgctgat
    32281 gctgcgtgac tctctgcaac gctgggttgc cagccagatc actggtgaag ttaccctgga
    32341 gctgcgccgt gggaacgatt attcaatcct gaataccgtc tcagagaacc tgacctacaa
    32401 gccagagcgt ctgacgatgg aaaaaggcga ctcggtgttc tcgccagatg atcgtatcgg
    32461 tcaactgacc atgcgcaacc tggatatcac cgatacccgc gagaaacttt tcggttatgc
    32521 caaaactggc ctgctttcct cctctgccgc ttcaggcgtg ccgcagatgg agaatctgga
    32581 aaacaaaggc cagtaattcg acatcaaccc tgcccttcag gggtggggtt accttgctta
    32641 tcctctgtaa actcctttct gagcatcaca taatcgaaaa catcatggtt tgcatccaca
    32701 acccgtgagt ctcctttata gtgtgcaatc acttcctcaa gcgtttgcgg ctgttccggc
    32761 tttgttaccc ccatccacgc attaataagg taattattgt aagctgtcga aaagatgttg
    32821 ttttccgttg tgcgatccac accatcccca agaacggggc tataccagat aaacatcggg
    32881 acatgatatg cctgctggct ggcctccctg cctccatgaa aatagacgtt cttcttcgta
    32941 gggtcacgtt ccaggccgtg gtcggcaaaa tacatgaccg aggcgcggcg atcttttaat
    33001 aattcaaaaa cctgacctag caaactatct gtgtaatgaa tggagttgtc atagcaggca
    33061 tcctgatcgt cctgcggttg aaacacggcg ctggattgcg gataggcgct acaagccggt
    33121 tcatggcttc catttaaatg aagaacaatc agtttctttt gctgcgtatt ttgctgtaat
    33181 gcttgcgata aatgcggcaa caacaattca tcaaatcctc tgacataaac tgtttccatg
    33241 gcacgcatgg cgatactggt aactgctgta ccattctgcc gaaaagcgga ttgcgagctt
    33301 agccagaaag tctgaaatcc tgcttgatta gccatattaa taatgttgtc ggggtaatta
    33361 tgaatgtcat ggctcaaaac agaatcagca gttaaagaaa ggggaaccga cagcgcagtg
    33421 taaggtgcac cgcttattgc ttgattaaac agcttgatct gttttctttg tgcttcgact
    33481 tgcggtgtcg tagagcgtgt atatccatac aaagacatat tgtcgacacg tacagactcc
    33541 cccacaatca acacgtaggt atcaatacct gtatccctga ctgataattg aaaatacggc
    33601 actgtgtttg caattgagag taatctttga tgctctttcg ctgctaaagc aaaataattg
    33661 agattgaaaa acggcgtata ggtagcaaat cgcgacgcta gtatatatgg actgaacgct
    33721 tttttatttt ttgcatcttt ataagcaaat tgacaagcgg aaaataaact tcccgaaatg
    33781 acaatcagca ataatattcc tgtcactttt tttgtcggca aggaaacatc atattttatt
    33841 attacagcca aaaaaagaag ggataaaaag gcaaaagcac atagataagg gctatacatt
    33901 cccagcattt tgacaacttc atccggatca ctctgcagca cactaatcgc aaatccatca
    33961 ttgaaagttg taccaaaggt acaccatgaa tagaggctaa tactcatgtc tgccgcagtc
    34021 agtaacacaa atggtgcggc aacaagcagg cgaagcggaa gggatgatat acgtttaaca
    34081 accaggatgg tcagaacgaa gaacagcaat cgctgaacca tatgcgctcc ggaagaggcg
    34141 actaagttcg taataccaaa aacaataaca ctaagataca acaaccaatg agatttaaaa
    34201 cttctagcaa atttgttgaa tactgtcatc cagaaatcca agttcacaac caatataaac
    34261 aattatcaac aatgtgcaat tttatatcag acatgttttt caactctgat ccatatcaga
    34321 acgaaacact gttagttttt tctttttaaa tgatgcggcc attgcgttct cagtaattaa
    34381 ataacattat caatgcgtct ctaaacatta cgcagaacgt agccatcagt agttatactg
    34441 acaggagcag gatggtttat cgagaagtaa acatatccgt attcgtaccg gaactggagt
    34501 tgacacaata aagtaccaat tatgtcagca gtagggaaaa gtttataacg aaggcgtacc
    34561 ggcaccatcg tttcaaggtg ccagctacga gtaaagcaac tggacgagat acagatacct
    34621 gacaaccatt cctcagacca ggaccaaaac gaaaaaagac gcttttcagc gtctcttttc
    34681 tggaatttgg taccgaggac gggacttgaa cccgtaagcc ctattgggca ctaccacctc
    34741 aaggtagcgt gtctaccaat tccaccacct cggcacggaa actacttttt agttcgggat
    34801 atcgctggtc ggcttagccg gagcagctgg ctgagtttgt tcggttttcg ccggcgcact
    34861 caggttttcc cattcgctac ctttattggt tttgttgcta ttgatgttac ccagcaccag
    34921 actgatgatg aagaataacg ttgccagcag cgccgtcatg cgggtcatga agttaccaga
    34981 accacttgaa ccaaacagcg tagcggaagc gcctgctccg aaggaggctc ccatatcagc
    35041 gcctttacct tgctgcagca tgattagacc aacaaggcca attgccacaa taaggaaaac
    35101 taccaaaaga gcttcgtaca taatcaacct gttccttgcg gagttgccgc gtaccaatgc
    35161 ttcaaccaat aaagcgggag tttataatct ttcccactga agcgggtgtg aatactaacc
    35221 aaagcgaatg accttcgcaa gggcaatttt atcgcattgt atcaactgcg gaaaaaaaca
    35281 gcaaaaccga tgtgttcgcc gagaaaaaag gcgacaatcg ccgccttttt agccagttat
    35341 ctaacgcttt aaacggcttt tactgcatcg gcgatgcggt gtgcaaattc agtcacctgc
    35401 gcttcgtctt cgccttccac catcacgcga attaacggtt cggtgccgga tttacgcaac
    35461 agcacacgtc cacggctacc cagcgccgct tcaacttctg cggtcaccgc tttgactgat
    35521 tcatgctcca gcggatcgcc gctacctgcg gtgtaacgca cgtttaccag gatttgcggg
    35581 aacattttca tgccgctgca aaggtcgtgc aggctcatat ggttacgcgc catcgccgcc
    35641 agcacctgca aaccagcaac gatgccgtca ccggtagtgg ttttatccag caggatcaca
    35701 tgaccggaat tctctgcacc gatacgccag cctttctcct gcattttttc cagtacgtag
    35761 cggtcaccca ctttcgcgcg cgcaaatgga attcctaact gtttcagcgc cagttcaagc
    35821 cccatgttgc tcatcaatgt acccacagcg ccaccacgca gctggccctg acgaagacct
    35881 tcacgcgcga tgatatacat gatctgatcg ccatcgactt tattgccttc atggtcaacc
    35941 ataatcacgc gatcgccgtc gccgtcgaag gcaataccga gatccgcttt ttcagccagc
    36001 acacgagcct ggagcgcgcg aacgtcggta gcccccactt cggcattgat gtttacaccg
    36061 tttggctcac aaccgatagc gataacattc gctcccagtt cgcgcagcac gttcggcgcg
    36121 atgtgataag tcgcaccgtt tgcgcaatcc accacaatct tcagttcact gaggctaagt
    36181 tcgttcggga acgtggcttt gcaaaactcg atatagcgac ccgcagcatc aacgatacgg
    36241 ctggctttac ccagttctgc cgaatcaaca cagctgatct ctttttccat ttccgcttcg
    36301 atggcttctt caactgcatc cggcagctta gtaccgtcaa tagaaaagaa tttaatgcca
    36361 ttatcgtaga acgggttatg cgatgcagat atcacaattc cggcctctgc gcggaaggta
    36421 cgcgtcagat aagccacggc cggtgttggc atcgggccag tgaagagtgc ggaaaggccc
    36481 gcagccgcca gccccgcttc cagtgctgac tccagcatat agccagaaat acgcgtgtct
    36541 ttaccaataa taatcttacg ggagccgtgg cgcgccagca ctttacccgc ggcccaaccc
    36601 agcttaagca caaaatcagg tgtgatcggc gcatccccta cacgaccacg aatcccatcg
    36661 gtaccgaaat atttacgatt actcatagcg tttgttttcc tttgcagaca gagtagcttc
    36721 caccactcgc atcgcttcta cggtttcttt gacgtcatga acacgaataa tgtgcgcgcc
    36781 ttgcattgcg gcaatgaccg cacaggccag actaccgctc aggcgctcgg acggccccac
    36841 gttcagcagc tgcccaatca tcgattttcg tgacataccc accaacagcg gcaggttgaa
    36901 atggtgaaat tcagccaggc gcgccagtaa tgaatagtta tgggagagat ttttaccgaa
    36961 accgaatccg gggtcgagca acaatttctc ttttgcgata cccgcctgct cgcaacgtgc
    37021 tatttgctca ataaagtagc gattcacttc tgcaaagaca tcgtcatact tcggggcttc
    37081 ctgcatggtt tttggatttc cctgcatatg catcagacaa accggtaaac cggtttctgc
    37141 agccgcctcc agagcgccag gttcggaaag ggagcggata tcgttaataa tgtgagcgcc
    37201 aactttcgct gactcacgga tgacttctgg tttggatgta tcgaccgaga tccagacttc
    37261 gaagcgttga gcaattgcct caaccacagg aataacacgt tgcaactctt cttcaacgct
    37321 aacttccgcc gcccctggcc gcgtggactc gccaccaacg tcaatgatcg tcgcgccagc
    37381 gttgatcatc agattcgcat gtttcaccgc atctatcagc gagttatgcg tgccaccatc
    37441 cgaaaaggaa tcaggcgtga cgttgaggat ccccattacg tgaggatggc taaggtccag
    37501 tgaagtaccc tgggcaaaga gtttcatgat gttatccctg gtatgaattg ataagaaaaa
    37561 accccggagc acgccccggg gttttcggta caaatacagt catctgatgc gggaacttac
    37621 ttgtcgccta actgctctga catggtgtta cccgggttcg gcgtacgcgg ttcatcaacc
    37681 ggacgtggag cctttggact accattgtcg ccagaattgt tagaagcgcc tggttcttcc
    37741 cagcccgctg gcggacgtac atcgcgacgt gccatcaggt catcaatctg cggtgcgtcg
    37801 atagtctcat atttcatgag agcatctttc atcgcatgca gaatatccat attgtcggtc
    37861 agaagctgac gcgcacgatt atagttacgt tcaatcagtg ctttcacttc ctggtcgatg
    37921 atacgtgcag tttcatcgga catatgtttc gctttcgcta cgctacggcc gaggaacact
    37981 tcaccttctt cttccgcgta cagcagtgga cccaatttct cagagaagcc ccactgagtc
    38041 accatgttac gtgccaggtt ggtcgcaact ttaatatcgt tggacgcacc ggtagaaaca
    38101 tgttccggcc cgtagatgat ctcttctgcc agacggccgc cgtacagcgt agaaatctgg
    38161 ctttccagtt tctgacggct ggcgctgatt gcgtcgccct caggcaggaa gaaagtcaca
    38221 cccaacgcac gaccgcgtgg gataatcgtc actttgtgca ccggatcgtg ctccggcacc
    38281 aggcgaccga taatcgcatg acccgcttcg tggtaagccg tcgattcttt ctgcgcttcc
    38341 gtcatcacca tggagcgacg ttccgcaccc atcatgattt tgtctttcgc tttctcgaac
    38401 tcaaccatcg acacaacgcg tttgttgcca cgagcagcga acagtgccgc ttcgttcacc
    38461 aggttcgcca ggtcagcacc ggagaaacca ggagtaccac gggcaatgat tgccgcgtcg
    38521 atatcgggtg ccaatggtac gcgacgcatg tgaaccttca ggatctgctc acgaccgcga
    38581 acatctggca agccaaccac aacctgacgg tcgaaacggc caggacgcag cagcgctggg
    38641 tcgagaacgt ccggacggtt agtcgcggcg ataacgatga taccttcgtt accttcgaag
    38701 ccatccatct caaccagcat ctggttcaga gtctgttcac gttcatcgtg accaccgccc
    38761 agaccagcgc cacgctggcg gcctacggcg tcgatttcat cgataaagat gatgcacggt
    38821 gccgctttct tcgcctgttc gaacatgtca cgaacacggg atgcacccac accgacgaac
    38881 atttctacga agtcagaacc ggagatagta aagaacggaa ctttcgcttc gcctgcaatc
    38941 gctttcgcca gcagcgtttt accggtaccc ggaggaccga ccatcaggac gcctttcggg
    39001 atcttaccgc cgagtttctg gaagcggctc ggctcgcgca gatactcaac cagttcagca
    39061 acttcttctt ttgcttcgtc gcagcccgca acgtcagcaa aggtcgtttt gatctgatct
    39121 tccgtcagca tgcgcgcttt gctcttacca aacgacatgg cacctttgcc accgccgccc
    39181 tgcatttgac gcatgaagaa gatccagaca ccaatcagca acagcatcgg gaaccaggag
    39241 atgaagatag aagccagcag gcttggttct tcaggcggtt caccgacaac cttgacgttc
    39301 ttggtcaaca ggttatccag tagtttcgga tcctgaaccg gaatgtaagt ggtataacgg
    39361 ttactatctt tcttggtaac gttgatttca cgtccgttga tacgcgcttc acgaacctgg
    39421 tcgttattga cctcttgtag gaaggtagag taatccacct tacggccatt agactcgctg
    39481 ggcccaaagc tctggaatac tgacatcagc acaacggcaa tgaccagcca gagtattagg
    39541 tttttcgcca tgtcactcaa gggattaacc tcttattaca actgtgttaa aaacagcgtc
    39601 aggatactct atatccagca tctttcaaac tttcgtctga aatctcccgg ttagggttta
    39661 cgcccggtcg ctacaatata cacttcccgc gaacgtgcac gagaagagtc cggcttacga
    39721 actttgacct tcgtaaacag ggagcgaatt tcccttagat actcatcgaa accttcgccc
    39781 tggaacacct tcactacaaa actgccacct ggcgctaata catcacgaca catttctagc
    39841 gccagttcca ccagatacat ggcacggggg atatccaccg ccggtgttcc gctcatgttt
    39901 ggtgccatat cggacatgac aacctggact ttgctgtcgc caacgcgctc cagcagtgct
    39961 ttcatcacca gttcatcacg aaaatcgccc tgaagaaagt ccacaccaac gataggatcc
    40021 ataggtaaaa gatcgcaagc gatgatgcgg cctttgccgc caatttgggt gaccacatat
    40081 tgtgaccaac cacccggagc agcaccaagg tcgacaaccg tcattcccgg tttaaagagt
    40141 ttgtcacttt gctgtatttc atcaagttta aaccaggcac gggaacgtaa cccctttttc
    40201 tgtgcctgtt gaacatattt atcgctaaag tgttcctgaa gccagcggct ggagctggca
    40261 gaacgcttct tacctgtcat ttaactttcc catggggata actcatcgta accaattgcg
    40321 taaattttta cgcgcctatt tggtgatata tgggagatgg cggtagaatg acccgttttc
    40381 aatcccaacg taagcaaaaa atacgatgaa tctgagtact aaacaaaaac agcacctgaa
    40441 aggtctggca catccgctca agccagttgt tctgcttggc agtaatggtt tgaccgaagg
    40501 ggtgctggcc gagattgaac aagcgttaga gcaccatgaa ctcatcaagg tgaaaatcgc
    40561 caccgaagat cgcgaaacta aaaccttgat cgtggaagct atcgtgcgcg aaaccggcgc
    40621 ctgtaatgta caggtcatcg gtaaaacgct ggtgctttat cgcccaacta aagaacgtaa
    40681 aatctcgctg ccacgctaag attatcctaa agttacacac atttgctgtg taaaacgagg
    40741 ggttttccgc aggcaggaga gcaaaatgcc acgctctgtt cgttgataaa aggccgcata
    40801 gcggcctttt tcctttcttt acaatacatc aacatcttga gtattgggta attcttacag
    40861 gtattccacc ttaattactt caaattctac ttcgccgccc ggcgttttga tgaccacaac
    40921 atcatcttct tctttgccga tcaggccacg agcaataggc gagtttacag aaatcaggtt
    40981 ttgtttaaag tcagcttcgt catcgccaac gatgcgataa gtctgttctt cgtcagaatc
    41041 cagattcagc accgttacgg tggcaccaaa aataacgcgc ccattgttgg gcattttggt
    41101 gacatcaatc acctgcgcgt tcgacagctt ggcttcgatg tctttaatac ggccttcgca
    41161 gaaaccctgc tgttcacgag ctgcgtggta ttcggcgttt tctttcaggt cgccatgctc
    41221 acgcgcttcc gcgatagcag caatgatttc aggacggcgc acagatttca gaaaatccag
    41281 ctcttcgcgt aatttttcag cgccgcgtaa ggtcatcgga atagcttgca tttgttatac
    41341 ctcttgaata ttcctgatgg ggcaagtttt cacccacccc ggctgttcgg cctgcccggc
    41401 ataactcctc accgggacca gaagcaaaaa aataccgacc cgggtacaag tcccaggtca
    41461 gctacaattc acattttgat agtcatttta ccctgaagtt cccgaagggt catcgtttac
    41521 tttatagggc gttgcgccgt agtatgacgg ctcgattcca ggttgttagc gcgagattat
    41581 gcgattttcc agatttatca tcggattgac cagctgtata gcgttcagtg ttcaggccgc
    41641 aaatgttgat gagtacatta ctcaactccc cgctggtgcc aaccttgccc tgatggtgca
    41701 aaaagtcggc gcgtcggccc ccgctattga ttaccacagt cagcagatgg cgctgcccgc
    41761 cagtacccag aaagtgatta ctgcgctggc ggcgttgatt caactcggcc ccgattttcg
    41821 tttcaccacg acgcttgaaa ccaaaggcaa tgtggagaac ggcgtactta agggtgactt
    41881 agtggcgcga tttggtgccg atccgacgct aaaacgtcag gacattcgta atatggtcgc
    41941 gactttgaaa aaatctggta tcaaccaaat cgatggcaat gtgttgatag atacctccat
    42001 tttcgccagc cacgataaag cccccggctg gccctggaac gatatgacac aatgctttag
    42061 cgccccgcct gccgccgcca ttgttgaccg caattgcttt tccgtctcgc tctacagtgc
    42121 ccaaaaaccc ggtgatatgg cctttgttcg cgtggcctct tattaccccg taactatgtt
    42181 cagccaggta cgcactctcc ctcgtggttc tgccgaagcg caatactgcg aactggatgt
    42241 cgtaccaggc gacctgaacc gctttacgct aacgggatgc ctgccacaac gttctgagcc
    42301 tctcccgttg gcttttgccg tgcaggatgg agccagctat gccggtgcaa ttctgaaaga
    42361 tgagttaaaa caggcaggta tcacctggag cggaacactg ctgcgccaga ctcaggttaa
    42421 cgaccccgga acggtagttg ccagtaaaca gtcggccccg ttgcacgatc tgcttaagat
    42481 tatgctgaaa aagtcggaca acatgatcgc cgatacggtt tttcgcatga tcggtcatgc
    42541 gcgcttcaat gtgcctggaa catggcgggc cgggtcggac gccgtacgtc agatcctgcg
    42601 ccagcaagcc ggtgtcgata ttggtaacac cattattgcc gatggttcag ggctttcgcg
    42661 gcataacctg attgcccccg ccaccatgat gcaggtgctg caatacattg cccaacacga
    42721 caatgaactt aactttatct ccatgttgcc gctggcgggc tatgacggct ctttacagta
    42781 ccgtgcaggt ctgcatcagg cgggcgtgga tggtaaagtc tcagcgaaaa caggttcgtt
    42841 gcagggggta tataacctgg cgggattcat taccacggcg agcgggcaac gaatggcatt
    42901 tgtacaatat ctttctggct atgcagtaga accagcagat cagcgtaatc gccgtattcc
    42961 gttagtgcga tttgaaagtc gcttgtataa agatatttat cagaataatt agtctaaaga
    43021 gaaaccccgg cacatggccg gggttttgct gattaacgct tgtaaatgaa ctcaacgcct
    43081 tcttcgtcgt cttcgtccca gtcgtcatcc cagtcttcat catcctcttc agcaatctct
    43141 tcaagctgct gacggtgata atcatcccac atgaattcga ctttctctgg ctgtttcgct
    43201 tcttcggcct gcaccactgg gttttcaatg ataaaggtca tcacatccca gcagagatct
    43261 ttcacgccca gtccgctcgc cgcagagatc agataatatt tatcttccca gcccagcgcc
    43321 tcagcgatcg ctttcgcttt ctcttcggct tctgccttat ccagcaggtc aatcttgttg
    43381 aagactaacc aacgcggttt cgccgccaga tcctggctgt atttttccag ctcgctgata
    43441 ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg gatcgatatc gatgaggtgc
    43501 aacaggacgc ggcaacgttc caggtgcttc aggaagcgaa tgcccagacc tgcgccttct
    43561 gcagcgcctt caatcagtcc tggaatatcg gcaacaacga agctcttttc gttgtccatt
    43621 cgtaccacac ccagacttgg caccagagtg gtaaacggat agtccgctac tttcggttta
    43681 gccgccgaga cagcgcggat aaatgtcgat ttaccggcat ttggcatccc cagcataccg
    43741 acgtcagcca gcaacatcag ctccagcagc agctcgcgct tatcgcccgg cgtgccattg
    43801 gttttctgac gtggtgtacg gttaacggac gatttgaaac gggtattgcc cagaccgtgc
    43861 cagccgccct tagcaaccag cagacgctga ccgtgtttgg tcatatcgcc catggtttca
    43921 ccggtgccct gatcgataac acgcgtacct actggtactt taatcgtcac gtctttaccg
    43981 cgcttaccgg tacaatctcg gcttgcgccg ttctggccgc gctctgcacg gaaagatttt
    44041 tcaaaacgat aatcgataag cgtgttcagg ttctcgtcgg cttccatcca tacgtcacca
    44101 ccgtcaccgc cgtcgccgcc atccgggccg cctttcggaa tatacttttc gcggcggaag
    44161 ctcacgcaac cattaccgcc atcacctgca acgaccagaa tcgatgcttc atcaacaaac
    44221 ttcattttat tctccgtaaa tcattcgcct gcgcggggtt gcgataccac cgttgtatgc
    44281 ttacgtaatc cgccccaaat acgatgacca atggcggaat acatcgcgcc cgcaaccacg
    44341 acaaacgcac cgagataacc taaaaggttt aacatcggtc tggcgaagaa atcgggccag
    44401 gccagtgata aaagatctga aaaaaacagc gtaaacagtg gggtgagtgt gattatcgca
    44461 ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca gggcgccata tcctaccaac
    44521 gtattcagtc cgcaaaaaat taaacatgcg agctgccagt ggctaagctg cgctatcact
    44581 ccaggctttg ccagagggaa gagcgcgatt gtacacaaag catacaataa aaacaggatc
    44641 tgcggtgagg ccagccgacg caatagtacc ttttgcgcca caccataaat cacccaaacc
    44701 gtcgccgcac cgaccccaaa aataacaccc caggtgtaat cggtaagttt tgtaaatatc
    44761 tcgatcagac tggtgttaaa gaacattacc aggccgctca ggagcatcaa tgcccccaca
    44821 atctgggtgc tgcgcatttt ctctttcaga ataaatacgc tggcaaccat catgccaacc
    44881 ggcgagagtt gcccaatcac ctgtgaggcg gtcgggctca agtattgcaa ggatgagctg
    44941 aacaggatga agttaccaaa cagcccggcg gtcgccactg ccagcaaaat caaccagcgt
    45001 ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca ccgcaagaat ggcaccaagg
    45061 ccaatacttg ccattaagaa acggtaaaat acgattgtcg gaggctccat tacctccagc
    45121 acctgcttca ttgcgattgg caacgcaccc cagcaaattg cggtagtgag cgccaaaaga
    45181 ataccaatgc ctgcctgctg cttcataccg taattcctgc aaaacgtatt tgcggattaa
    45241 cgccgcgccg tgtgcatttt tactggttat cgaatgtaaa aagccccgca acacgttgcg
    45301 gggctttcat ccgttaccgg gacgcgaaaa acttattcag cttcgatgct gataaattta
    45361 cggtttttcg ggcctttaac ttcgaatttc actttaccgt ctgctttagc aaacagagtg
    45421 tggtcacgac cgcaacctac gttagcgcca gcgtggaatt tggtaccacg ttgacgaaca
    45481 ataatgctac ccgccagaac ggattcgcca ccgaaacgct taacgcccag gcgtttagct
    45541 tctgaatcgc gaccgttacg tgtggagccg ccagcctttt tatgtgccat ttgaaatctc
    45601 tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc agtgaaccac tgacgatggc
    45661 cctgctgctt acgatagtgt ttacgacgac gaaacttaac gattttaact ttctcgccac
    45721 gaccgtgagc aacaacttca gctttgatta cgccgccatc aacgaaagga acgccgattt
    45781 tgacttcttc accgtttgcg atcatcagca cttcagcgaa ctcaacagtt tcgccagttg
    45841 cgatgtccag cttttccagg cgaacggtct gaccttcgct tactcggtgt tgtttaccac
    45901 cactttggaa aaccgcgtac atataaaact ccgcttccgc gcacaccttt tcaatgattc
    45961 agagtgcgct ataaatattc acaatagggc gcgaatatta cgcaaaacgc acgcctttga
    46021 caagtgctac agtcaataca cgaagaaaaa aaacacaact tgtacggtaa cgtttatctg
    46081 tgccattttt tcagtacaat caccctatat tcctaaccat aaaccctaag ttgcctttgt
    46141 tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg atgaatttag aaaaaatcaa
    46201 tgagttaacc gcgcaagata tggcgggtgt taatgcggca atccttgagc agcttaattc
    46261 cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc agcggcggcg gtaaacgtat
    46321 tcgtccgatg attgctgtac tggctgcacg agctgttggc tatgagggaa atgcgcatgt
    46381 caccattgct gccctgatcg agtttatcca cacggcgact ctgctacatg acgacgttgt
    46441 ggatgaatca gatatgcgca ggggtaaagc taccgccaac gccgcatttg gcaatgccgc
    46501 cagcgtgctg gtaggcgatt ttatttatac ccgcgctttc cagatgatga ccagcctcgg
    46561 ttcgctcaaa gtgctggaag tcatgtcaga agccgtaaac gtcatcgcag aaggtgaagt
    46621 tctgcaactg atgaacgtta acgatccgga catcactgaa gataactaca tgcgcgttat
    46681 ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag tgttccggga ttctggctgg
    46741 ctgtacgccg gaggaggaga aaggcctgca ggattatggg cgctatctcg gcactgcttt
    46801 ccagttgatc gacgatttac tcgattacaa tgccgatggc gaacagttag gtaaaaacgt
    46861 cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg ctgcatgcga tgcatcatgg
    46921 cacaccagaa caggcacaga tgatccgtac cgccatcgaa cagggtaacg gtcgccatct
    46981 tctggaaccg gttctggaag caatgaacgc ttgtggatct cttgaatgga cgcgtcagcg
    47041 cgccgaggaa gaagcagaca aagccatcgc agcgttacag gtgctcccgg acaccccttg
    47101 gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa cgcgatcgtt aatcccctcc
    47161 cctcatcccg cgcagtgcgc gggatgattc cagtaaactc cataagaata cctattcagc
    47221 gtaataatgc actgaaaaac cagtgtatac atctttatat attctgaata ttcacactct
    47281 ttacaggaac tttttagagc aatcggccat caggagtata gtgatgctcg acagaagaag
    47341 tgttctgaat gaaagcgact aacttaagga gtgaggaaag atggaaagta atttcattga
    47401 ctggcatccc gctgacatca ttgcgggttt gcgcaagaag ggaacgtcaa tggcggcgga
    47461 gtctcgcaga aatggtttga gttcctcaac gctggcgaac gcattatcgc gcccatggcc
    47521 gaaaggagag atgattattg cgaaggccct gggaactgac ccctgggtta tctggccatc
    47581 acgctaccat gatccgcaga cccatgagtt tatcgacaga acgcagttga tgcgtagcta
    47641 cactaaaccg aaaaaatgag tggtctggcg gtagccccgc gaacggggct gccagctttc
    47701 agacgattac tcgcctttca cacgctcaat atttgcacct aaagcgcgca gtttgtcttc
    47761 aatgcgttcg tagccacgat cgatgtgata aatacgatca accaccgtcg tcccttccgc
    47821 aatacagcca gccagcacca ggcttgctga tgcacgcaga tcggttgcca taacctgtgc
    47881 gccagaaagt ttttcaacac cgtgacaaat aacggtattg ctttcgattt cggcgtgtgc
    47941 gcccatacgg ctcagctctg gcacatgcat aaagcggttt tcaaagaccg tttcggtgat
    48001 gaatccggtc ccttcagcca ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg
    48061 gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc catgcatatc
    48121 caggctaatc cagtcttcgc cgacttcgat gtccgctcca gcatcacgca gtttcgccag
    48181 cacggcgtcg agagtatctg gctgcgcgtt acggcagata attttgccgc gagaaatcgc
    48241 cgccgccacc aggaaagtac cggtttcgat acgatccggt agcacgcgat agacaccgcc
    48301 gcctaaacgt tccacacctt cgatgacgat acgatcggtg ccctgaccgc taattttcgc
    48361 acccagcgta atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg ctgcgttttc
    48421 aataatcgtg gtgccttccg ccagggttgc agcacacatg atggtcaccg ttgcgccaac
    48481 gctgacttta tccatcacga tatgtgcgcc tttcagacga ccatcgacgg aagctttaac
    48541 gtaaccttct tccagtttga tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc
    48601 aaccggacgc gcaccgatcg tacaaccgcc aggtagtgaa acttgcccct gaccaaagcg
    48661 cgctaccagc ggccccagtg cccagataga agcacgcatg gttttaacca gatcgtaagg
    48721 tgcgcagaat acattaacgt cgcgggcatc aatatgcaca gaaccattac gttctacttt
    48781 cgcacccagc tggcttagca gcttcattga tgtatcgacg tctttcagtt tcgggacgtt
    48841 ctggatctct accggttctt ccgccagtag agcggcaaaa aggataggca gagcagcatt
    48901 tttagcgccg gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt
    48961 atccatttag tttgttctca gttaacaatt catatccgct accggcgaat cgcccatagc
    49021 tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg ctttgatcga
    49081 cacagcatga atgcggttat ccgcaatata ttccatcagc ggaccataga ccgtctgctg
    49141 ttttttaacc cgactcatgc cgtcaaacaa ctcacccacg gcaataacct gaaagtggct
    49201 gccatcgccg gaaacgtgga cttcctggag ggagagagcg ttcatcaaca cgctctgaat
    49261 ttcattattt tccatgggat cttcaatcat cagttaataa accagcgaaa catcttagag
    49321 caaagttgcg ctggcataaa taagcaaaaa gcctcgctga taaatcagac aaggctcgac
    49381 ttgcaggcag gtttgccgga caggcggtta acgccatatc cggcctgaaa aaatttaacg
    49441 aggcagcaca tcagcaggca aattatacaa tttcgccagg gtatacactt tgtcgtttac
    49501 cccctgaagc gtcacattgt tgccctgctt tttcgccaga tcgataagat ggagcagcag
    49561 tgccagtccc cccgtatcca cgcgggacac acggctaaga tcgatgcagg taaccccctt
    49621 caccgcttcc tcacgcattt cccaaagcgg tagcagaacg tcctgatcca gctctccgga
    49681 taacgccagc gtgtcacccg tctgcatcca gctcagtgac tcgctcatta ttttttctct
    49741 tccagagtga ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct
    49801 ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg tgatcatact gacgccttca
    49861 gcaatcatgt cgtaagcctg ccagttgccc gtctgggagt ttttacgcca ctggaagtcc
    49921 agacgcaccg gcgggcggcc attcgggtca ataatggtaa cgcgaatagg cacaatggtt
    49981 ttatcaccca gcggctgttc tggcgcaatc tgataggttt gaccgtgata catcgccagc
    50041 gcctgaccgt aagcctgctt caggtactca cggaaagcgg caaagtaagc atcacgctgg
    50101 gcgggagtcg cactcttgta atactggccc agcaccagcg caccggcgta tttcacctgt
    50161 acgtatggca gcagttcctg atcaacaatg gtacgcagat aatccgggtt ggcccgaatt
    50221 tgcggttgct cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc catcagctta
    50281 tacggattgg tctggtctgc cgcggttgcc gcactcagcg gtgcaatcac cagcaaagcg
    50341 accatcatta aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt tgtacccaca
    50401 ggttcagtgg tttcattatt acctggcgca gcagctggcg catcgccact attcttattg
    50461 tcatcgcctt tactaccgta aaggaactga ccaatgagat cttccagcac catcgcagac
    50521 ttggtgtcct gaattgtatc gccatccttc aggatagcag tccccagttc cgggtcttca
    50581 aaaccgacgt ttaatgccag atattgttct cccagcaggc cggaagtacg aatgctcagc
    50641 gaactggtat caggaatgtg gttataacgt tgttcaattt ccagcgttac gcgtggcaga
    50701 taggttttcg ggtccagcgt aatatccgcc acccggccca caacaacacc accaatgctg
    50761 accggagagc gcgctttcag gccgccaatg ttatcgaacg tcgcataaag cgtgtaggtc
    50821 ggttcagtac gtattgacgt cacgttcgcc gccttcaggc aaacaaacag cgccgccagc
    50881 agtgctgcta ataaaaagat acccacccaa atttcatttt ttttcgtttg catgaactca
    50941 attcccaaac atcaatgcgg tcagcacaaa atccagcccc agaacagcca gagacgagtg
    51001 gacaacggtg cgagtggttg cccggctaat cccggcagac gtcgggatgg catcgtagcc
    51061 gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt
    51121 gaccagatcc atacgccagt cgacggcatt ttgcattgcc gaccagaaga acccgctatc
    51181 aatgcctttc caactgacgc cgaccagcga tccgccccag atccccacgg cgacgaaaat
    51241 aaccgtcaac agtggtaatg aaataacccc agcccagaaa cggggagaaa taacccgacg
    51301 cagcggatcc accgccatca tctccatact ggagagttgc tctgtagcgc gcatcaggcc
    51361 gatttctgcg gttagcgccg aaccagcacg cccggcaaac aacaacgcgg caaccaccgg
    51421 ccccagttca cgcagtagcg ataacgccac cagcataccc agactggttt ccgcactata
    51481 agtggtcaga accagataac cttgcagccc caacaccatt ccgatgaaca cgccagaaac
    51541 tacaataatc agcatcgaca ggacgccgac attatagagc tggcgcacca gcagcggcgc
    51601 atgtttgcga aattccggtt tgccgaccag cgcattgaat aacattaacc cggcccgccc
    51661 gaacgttctc agggttttaa tccctttatg tccgagcgac gccagcgcat ttaacagcat
    51721 gagtggctta actccctggt aaaagatcag cgtgataatc gccggcagga tagcggaacg
    51781 gaacaggccc gtcagctatc ccgtccagaa actgacgtac gcgcggatca ggattcgcct
    51841 gcaacgcctt ggcactgcca tgagcgacaa tttttttgtc cgccaggatc caggcgtgat
    51901 ccgcaatact taacacttcc ggcacatcgt gagaaaccac cacacaagtc acgcccagcg
    51961 cgctgttcag ctcagaaatc agcttcacca gtacgcccat ggtaatggga tcttgcccaa
    52021 caaagggctc atcaaacatg atgagatccg gctccagcgc aatcgcacgc gccagcgctg
    52081 cacgccgcgc catcccaccg gaaagttcag aaggcattag tttagccgct ccacgcagcc
    52141 ccacggcctc cagcttcatc atcaccgtac tatgtaacaa tggcgcggga agttgggtat
    52201 gttcgcgcag tggataggcg acgttgtcaa atacgttcat atcagtgaac aacgccccgg
    52261 actgaaataa catgctcatc cgtttgcgca ctgtatacag gcgcgaacga gacatcgccg
    52321 gaatattctc accatcgaaa aggatctcac catgatctgg tgcgatttgc ccgccaatca
    52381 gacggagtag cgtcgtttta ccgatgcccg atggccccat gatcgccgtg atcttccctc
    52441 gcggcacggt cagggaaata ttatcgaaga tgcagcgatt gccacgcgta aaactgacat
    52501 cgcgcatatc gactaaattc gccacagact gctccataat tcacccttcg tcttgcgttg
    52561 attttctaag catggcgctc aatttaacct tgaacccaac atatttacag aatattaccc
    52621 gccgtggtta gcgaaagctg gcatttgttt tactttttag ccgcataaag tcaaaattaa
    52681 gaatccgtta cggctttctg aaaatcttca gcggaccggc gagtatacct gaagaaagga
    52741 cgttagatgc ttttagctac ggcactgtta attgttggtt tacttttggt cgtttacagt
    52801 gccgatcgcc tggtttttgc cgcgtctatt ctttgccgaa cctttggcat cccgccgctg
    52861 atcatcggca tgacggtggt cagtattggt acatcgttac cagaaatcat cgtctcgctt
    52921 gccgcgtctc tgcacgaaca acgcgattta gccgttggta cagccctcgg ctcaaacatt
    52981 atcaatatat tgctgatcct cggtctggct gcgctggttc gtccttttac cgtccattct
    53041 gatgttctac gccgtgaatt acccttaatg ttgttggtca gcgtggtggc cggttccgta
    53101 ctctatgacg gacaacttag tcgcagcgat ggtatctttc tcctctttct ggctgtgcta
    53161 tggctgctgt tcattgttaa acttgcacgt caggctgaac gtcaggggac tgacagcctg
    53221 accagagagc agcttgcaga gctgccgcgt gacggcggat tgcccgtcgc gtttttatgg
    53281 ctcggcattg cgcttatcat catgccagtg gccacgcgga tggtggttga taacgccacg
    53341 gtgctggcga attactttgc catcagcgag ttgacgatgg gtctgacggc aattgctatc
    53401 ggaaccagcc tgccggaact ggcaaccgca atagcggggg ttcgcaaagg tgaaaacgac
    53461 attgctgtcg gaaatatcat tggcgcaaac atttttaata ttgtcatcgt gttgggttta
    53521 cccgcgctga taacgccagg agagattgat ccactggcgt acagtcgtga ctacagcgtg
    53581 atgttgctgg tgagcattat ttttgcgttg ctgtgctggc ggcgctcccc gcaaccgggc
    53641 cgtggtgtag gggtattatt aactggcgga tttatcgtat ggctggcgat gttgtactgg
    53701 ttatcgccaa tactcgttga ataactggaa acgcattatg tcgcacgtag agttacaacc
    53761 gggttttgac tttcagcaag caggtaaaga agtcctggcg attgaacgtg aatgcctggc
    53821 ggagcttgat caatacatca atcagaattt cacgcttgcc tgtgaaaaga tgttctggtg
    53881 taaagggaaa gttgtcgtca tggggatggg gaaatcgggg cacatcgggc gcaaaatggc
    53941 cgcaacgttt gccagcaccg gtacaccttc atttttcgtc catcctggtg aagccgcgca
    54001 tggtgattta ggcatggtca ccccacagga tgtggtgatt gctatctcta actcaggtga
    54061 atccagcgaa atcactgcct taattccagt gcttaagcgt cttcacgtac cgttaatctg
    54121 catcaccggt cgcccggaga gcagcatggc gcgcgccgca gatgtgcatc tgtgtgttaa
    54181 agtagcgaaa gaagcctgtc cgttagggct ggcaccgacc agcagcacca ccgccacgct
    54241 ggttatgggc gatgccctcg ctgtcgcgct gttaaaagca cgcggcttta ctgctgaaga
    54301 ttttgcgctc tcacacccag gcggcgcact gggtcgtaaa cttctgctgc gcgtaaacga
    54361 tattatgcat acgggcgatg agatcccgca tgttaagaaa acggccagtc tgcgtgacgc
    54421 gttactggaa gttacccgca aaaatcttgg tatgactgtc atttgcgatg acaatatgat
    54481 gattgaaggc atctttaccg acggtgattt acgccgtgtc ttcgatatgg gcgtggatgt
    54541 tcgtcagtta agtattgccg atgtgatgac gccgggggga atacgcgtgc gccctggcat
    54601 tctggccgtt gaggcactga acttaatgca gtcccgccat atcacctccg tgatggttgc
    54661 cgatggcgac catttactcg gtgtgttaca tatgcatgat ttactgcgtg caggcgtagt
    54721 gtaaagattc aaggataaac aacaatgagc aaagcaggtg cgtcgcttgc gacctgttac
    54781 ggccctgtca gcgccgacgt tatagcaaaa gcagagaaca ttcgtctgct aatcctcgac
    54841 gttgatggcg tactgtcaga tggcctgatt tatatgggca ataacggcga agagctgaaa
    54901 gcgttcaatg ttcgtgacgg ttatggcatt cgttgtgcgc tcacctctga tattgaagtc
    54961 gctatcatta ccgggcgaaa ggctaaactg gtagaagatc gttgtgccac attggggatc
    55021 actcacttgt atcaggggca gtcaaacaaa ctgatcgcct ttagcgatct gctggaaaaa
    55081 ttggcgattg ccccggaaaa tgtggcttat gtcggcgatg atctcatcga ctggccggta
    55141 atggaaaaag taggtttaag cgtcgccgtg gccgatgcgc atccactgtt gatcccgcgc
    55201 gccgattacg tgacgcgcat tgctggcggt cgtggcgcag tgcgcgaagt ttgcgactta
    55261 ttactcctgg cgcagggcaa actggatgaa gccaaagggc aatcgatatg agtaaagcca
    55321 gacgttgggt tatcattgtg ctatcactgg cggttctggt gatgatcggc attaatatgg
    55381 ccgaaaaaga cgataccgcc caggtggtcg tcaacaacaa tgatcccacc tataaaagcg
    55441 agcatacgga cacgctcgtc tataacccag aaggggcgct aagctatcga ttgattgctc
    55501 aacacgttga atattattcc gatcaggccg tttcgtggtt tacgcagccg gtacttacca
    55561 cgtttgataa ggataaaatc ccgacatggt ccgtaaaagc agataaagcc aagctgacca
    55621 atgaccggat gctctattta tatggacacg ttgaagtcaa cgcactcgtg ccggactctc
    55681 aacttcgcag aatcacgacg gataacgcgc agatcaatct ggtgacgcag gatgttacct
    55741 ctgaagacct cgtcacgtta tacggaacaa catttaactc cagcggtctg aaaatgcgcg
    55801 gcaacttacg cagcaagaac gccgagctga ttgaaaaggt tagaacatcc tatgaaattc
    55861 aaaacaaaca aactcagcct taatcttgtg cttgccagct cacttctggc cgccagcatt
    55921 ccggcatttg ccgtaaccgg agacactgat cagccgatcc acattgaatc ggaccagcaa
    55981 tctcttgata tgcaaggcaa cgtggttacc tttaccggta atgtcatcgt cacccagggc
    56041 accatcaaaa ttaatgccga caaagtggtc gttacccgtc cgggcggcga acaaggtaaa
    56101 gaagtgattg acggctacgg taaaccggca acgttctacc agatgcagga caacggtaaa
    56161 cccgttgaag gtcacgcttc ccagatgcac tacgaactgg caaaagattt tgtcgttctg
    56221 acgggtaatg cttatctgca gcaggtcgat agcaacatta agggcgataa gatcacttac
    56281 ctggtgaaag agcagaaaat gcaggctttc agcgacaaag gcaagcgtgt aaccaccgtt
    56341 ctggtgccgt cgcagctgca ggacaaaaac aacaaaggcc agaccccggc acagaagaag
    56401 ggtaattaat tcgttatggc aacattaact gcaaagaacc ttgcaaaagc ctataaaggc
    56461 cgtcgcgtgg tagaagacgt cagcctgacc gtcaactccg gggaaattgt cggtctgctg
    56521 gggccaaacg gtgccggtaa gaccaccact ttctatatgg ttgttggcat tgtgccgcgc
    56581 gatgcgggca acatcattat tgatgatgac gatatcagtc tgctgcctct gcatgcacgc
    56641 gcgcgccgcg gtatcggcta tctgccacag gaagcctcca ttttccgtcg cctcagcgtt
    56701 tacgataacc tgatggcggt actgcaaatt cgtgacgact tgtctgctga acaacgtgaa
    56761 gaccgcgcga acgagctgat ggaagagttt cacattgagc acctgcgtga cagcatgggg
    56821 cagtcactct ccgggggtga acgtcgccgt gtagaaattg cccgcgcact ggctgcgaat
    56881 ccgaaattta ttctgctcga cgaaccgttt gccggggttg acccgatctc ggttatcgac
    56941 attaaacgca tcattgagca cctgcgcgac agcggcctgg gcgtgctgat cactgaccac
    57001 aacgtgcgtg aaacactggc ggtttgtgaa cgcgcttata tcgtcagtca ggggcatttg
    57061 atcgcccacg gcacgcctac agaaatctta caagacgaac acgttaagcg tgtatacctt
    57121 ggggaagact tcagactctg atagggtaga agtttgcgac gttttagcag gagagtacga
    57181 ttctgaacat gaagcaaggt ttgcaactca ggcttagcca acaactggcg atgacgccac
    57241 agctccaaca ggcaattcgt ctgttgcagt tgtcgacgct ggaacttcag caggagctac
    57301 agcaggcgct ggagagtaat ccgctgcttg agcaaatcga cactcatgaa gaaatcgaca
    57361 cccgcgaaac gcaagacagt gaaacgctgg acaccgccga cgcgctcgaa caaaaagaga
    57421 tgccggaaga gttgccgctc gatgccagtt gggacaccat ttacaccgct ggtacaccat
    57481 ccggcaccag cggtgactac attgacgacg agctaccggt ctatcagggc gaaacgacgc
    57541 agaccttgca ggattacctg atgtggcagg tcgagctgac accgttttcc gacactgacc
    57601 gcgctattgc tacctctatc gtcgatgccg ttgatgacac cggttatctg actgtcccgc
    57661 tggaagatat tctcgaaagt atgggcgatg aagagatcga catcgacgaa gttgaagccg
    57721 tccttaagcg gatccaacgg tttgatccgg tcggtgtggc ggcaaaagat ctgcgtgact
    57781 gcctgctgat ccaactctcc caattcgata aaaccacgcc gtggctggaa gaggccagac
    57841 tgatcattag cgatcatctc gatctgttag ccaatcacga cttccgcact ttaatgcgcg
    57901 tcacgcgtct gaaagaagat gtgctgaaag aagccgtcaa tctgatccag tcgctcgatc
    57961 cgcgccccgg gcagtcgatc cagactggcg aacctgagta tgtcattcca gatgtgctgg
    58021 tgcgtaagca taacggtcac tggacggtag aactcaacag tgacagcatt ccgcgtctgc
    58081 aaatcaacca gcactacgcc tcgatgtgca ataacgcgcg caacgatggt gacagccagt
    58141 ttatccgcag caatctgcag gatgccaaat ggttgatcaa gagtctggaa agccgtaacg
    58201 atacgctact gcgcgtgagt cgctgtatcg ttgaacagca gcaagccttc tttgagcaag
    58261 gcgaagaata tatgaaaccg atggtactgg ccgatatcgc ccaggctgtc gaaatgcatg
    58321 aatcgacgat atctcgcgtg accacgcaaa aatacctgca tagtccacga ggcatttttg
    58381 aactgaagta tttcttttcc agtcacgtca ataccgaggg cggcggcgaa gcttcctcta
    58441 cggcgattcg tgcgctggtg aagaaattaa tcgcggcgga aaacccagcg aaaccgttga
    58501 gcgacagcaa gttaacctct ttgctgtcgg aacaaggtat catggtggca cgccgcactg
    58561 ttgcgaagta ccgagagtct ttatccattc cgccgtcaaa ccagcgtaaa cagctcgttt
    58621 gacccaaccg ataaggaaga cactatgcag ctcaacatta ccggaaataa cgtcgagatc
    58681 accgaggcac tgcgcgaatt tgttacagcc aaatttgcca aacttgagca atattttgac
    58741 cgaatcaacc aggtctatgt tgttctgaaa gtggaaaaag tcacccacac ctcagatgca
    58801 acactgcatg taaacggtgg tgaaattcat gccagcgcgg aaggtcagga catgtacgct
    58861 gccattgatg gcttaattga taagctggca cgccagttga ccaaacataa agataaactg
    58921 aaacaacact aattgtccgg gcaattagca tgtgcatggc ggcctgttgt gcggcacaac
    58981 gggccatttg tacggttaat gctccgagcc tgttccactg tttgagtggg caggttctta
    59041 ggtgaaatta tgacaaataa tgatacaact ctacagctta gcagtgttct taacagggaa
    59101 tgtacgcgaa gccgcgtcca ctgtcagagc aaaaaacgcg ccctggaaat catcagcgag
    59161 ctggcggcga aacaacttag cctgccgcct caggtggttt ttgaagctat cctgacgcgg
    59221 gaaaaaatgg gcagtaccgg tatcggcaat ggtattgcca ttccgcatgg caaactggaa
    59281 gaagatactc tgcgtgccgt tggcgttttc gttcagctcg aaacgcctat agctttcgat
    59341 gccatcgaca accaaccggt ggacttactt tttgccctgc tggtgccggc agaccaaact
    59401 aaaacgcacc tgcatactct gtcgctggtg gcgaaacgtc tggcggacaa aaccatctgc
    59461 cgccgtttac gcgcagccca gagcgatgaa gagctgtatc aaatcattac ggataccgaa
    59521 ggtactccgg atgaagcgta gttattcggt aatgtctctt ttagacgttg tgaggagaaa
    59581 cagtacatgg tactgatgat cgtcagcgga cgttcaggtt caggtaaatc tgtcgccctg
    59641 cgtgcgctgg aagatatggg tttttactgc gtggataacc ttcccgtagt gttgttaccc
    59701 gatctggctc gaaccctggc cgatcgcgag atttctgccg ccgtcagcat tgatgttcgt
    59761 aatatgccgg agtcaccaga aatattcgaa caggcgatga gtaacctgcc tgacgctttc
    59821 tcaccgcaac tcctgttcct ggatgccgac cgtaatacct taattcgtcg ttacagtgac
    59881 acgcgccgac tgcatccgct ttccagcaaa aacctgtcgc tggaaagtgc tatcgacaaa
    59941 gaaagcgatc tgctggagcc tctgcgttcg cgagcggatc tgattgtcga tacctcagaa
    60001 atgtccgttc acgagctggc agaaatgctg cgtacccgtc tgctgggtaa acgtgaacgc
    60061 gaactgacaa tggtctttga gtctttcggc ttcaaacacg gtatccctat cgatgcagat
    60121 tacgtctttg acgtgcgctt cttgccgaac ccgcactggg atccgaaact gcgtccaatg
    60181 acaggtcttg ataagcctgt cgccgcgttc ctcgaccgcc acacagaagt acacaatttt
    60241 atctaccaga cgcgaagcta tcttgagcta tggttaccta tgctggaaac caacaaccgt
    60301 agctacctga cggtcgccat tggttgtacc ggcgggaagc accgttcggt gtatattgca
    60361 gagcaactgg ctgactactt ccgctcgcgc ggtaaaaacg tccagtcacg ccatcgtacg
    60421 ctggaaaaac gtaaaccatg accgtcaagc aaactgttga aatcacaaac aagctgggca
    60481 tgcatgcccg gcctgcaatg aagctgtttg aattaatgca gggttttgac gctgaagtgc
    60541 tgttacgtaa tgacgaaggc accgaggctg aagccaacag cgttattgcg ctgctgatgt
    60601 tggattctgc caaaggacgg cagattgaag ttgaagcgac cggtccacag gaagaggaag
    60661 cactggccgc cgttatcgcc ctctttaact ctggttttga tgaagattaa tcttcatcac
    60721 tttgacattc aaatcccttc aagctccccc ggcaataaga aaagcgcgta ctgaaatcgt
    60781 tctcaatcaa cgtcaattgt acattttgtg cgcttttcac tttcagaaga accttaagaa
    60841 aaccttaaga ggcattgttt aggttttgtt taagttaatc gaccatactg gagatcgtca
    60901 gaaaatattt ccaggagatg gcatgattcg cttatctgaa caaagtcccc tgggcaccgg
    60961 aagacatcgg aaatgttatg cgcatccgga agatgcccaa cgctgtatca agattgtcta
    61021 ccatcgtggc gatggtggcg ataaagagat ccgtcgtgag ttaaagtact acgcgcatct
    61081 tggtcgccgg ttaaaagact ggagtggaat accgcgctat cacggtaccg tcgagacgga
    61141 ttgcggaacc ggttatgttt atgatgtgat tgccgatttt gacggcaagc cctccattac
    61201 gctgaccgaa tttgctgaac aatgccgtta cgaagaagac gttgcacaac tgcgccaatt
    61261 actgaaacag ctgaagcgtt atttgcagga taaccgtatc gtgacgatgt cgctgaagcc
    61321 gcagaatatt ctctgtcatc gcatcagtga gtcagaggtt atccccgtcg tctgcgacaa
    61381 cattggtgaa agcacattga ttccactggc gacctggtcg aagtggtgtt gtctgcgtaa
    61441 gcaagagcgg ttatggaaac gatttatcgc ccaaccggcg ctggctattg ccctgcagaa
    61501 agatctgcaa cctcgtgaga gcaaaacgct ggccctcact tcgcgcgaag cttaatccag
    61561 ctggtgttgt tgcataaacg gctcaccgcc taactgatac atctgccgta aaatccacgc
    61621 ctgacggctg cgcacgtagc ccgatggtgc ggagactttg aaacgaagtg gattaggtaa
    61681 tacagctgcc agtaaggcag cttccgatcg ggtaagtttg ctcgcgggtt tgtggaaata
    61741 gcgttgtgcc gcagcttcga cgccaaacac accgtcgcca aattcggcga tattcaggta
    61801 aaccgtcagg atacgctttt tgctccagac tgtttctatc cccagcgtta atccggcttc
    61861 cagccctttt cgaacccagc tacgcccatc ccataaaaag agatttttgg ccgtctgctg
    61921 agaaatcgtt gaagcaccgc gaatacggtt ttcattgcgc tcgttgtgcg ccagggcttt
    61981 ctcaatggaa gcaacatcaa agccccagtg ctcaggaaat ttctgatctt ctgcggcaat
    62041 caccgccagt cccatccacg gcgagatttg atccatactg acccagtcag aatgtgccac
    62101 gtaacgaaaa ttgccatgca gccaggcgct gacctgtcgc tcgaccatta ccgctgagaa
    62161 aggaacaggc gcaacgctaa acaacgcgat gcccccgccc cagaaaacgg cgaggacaac
    62221 cattaaccgc agaagaaaac ggcgaacgaa actaaacacc gttaagcggc ttttactcat
    62281 tcagccagaa ccagcacgcg ggaaaccagc ttatcaatgc cgctcgccgc ttctgcaatg
    62341 ttctgcgcca gcatatatgc cggggtggtg acaatcttat tgtcttcatc aaccacgata
    62401 tcatcgacag gacacggcac atgctccgcg cccatctctt ccagcacttc tgcggtatcg
    62461 atatcagtac cgatggtcaa acgcagcggg aaatcgaaaa ttttcggcag catcgccggg
    62521 gcaatacaca taaaaccaag cggttttccg gcctgatgca tcgcttgtgc cagcgccttt
    62581 aattcacggt caacggtgca ttcgcgacca agactggcaa aattgcttaa attctttgcc
    62641 gcgccaaacc cccccggcac aatcaacgca tccagttcag cggcatcggc ctgggccaga
    62701 ggacggattt caccgcgcgt tattcgtgcc gcttcaatca gcacattgcg cgtttccgtc
    62761 atcgcttcgc cagttaaatg attgataaca tcaacctgct gcttatccgg tgcaaagcag
    62821 accgcctgcg caccgctgcg tgaaatagct aacaacgtca acaccgcttc atgaatttca
    62881 gaaccgtcat agacgccgca tccgctcaga attacgccaa ttttcttcat tgtgatcatc
    62941 ctttcgcaat tgactgaaac acattaattt ttttaataaa aatggtacgc atcacacatt
    63001 taactgattc atgtaacaaa tcatttaagt tttgctatct taactgcgtg cggcctgaaa
    63061 aacagtgctg tgcccttgta actcatcata ataatttacg gcgcagccaa gatttccctg
    63121 gtgttggcgc agtattcgcg caccccggtc tagccggggt cattttttag tggcttttgc
    63181 cacccacgct ttcagcactt ctacgtcgtg acgccactct tctttcatct cttcaatcca
    63241 ttcaccgacg ttatcttccc aggcaggaag gtcaggagac tgaatttgct gacccagctg
    63301 ttgcagatgg cgtaacccca ctgaccccgc cgcaccttta attttatgtc cttcctcaac
    63361 aatgcctttt ttatcctgcg ccgtcagatt cgactccagc acgctgacat agcccggcat
    63421 catcttctca aacaccgcta acccgtcggt gatcagcttc ggtcctacaa gttcgagata
    63481 ctgttccagc atgggaatat cgagcaatgc ttctgattta ctgttctctt ctgtcgtcac
    63541 cgtactctcc tcatcatcct gggtatccca gaatttcttg atcatcgcgg ttagcgccgg
    63601 aacagaaagc ggcttactca gcacatcatc cattccagca ttgaggtatt cttgtttgtc
    63661 tttcaacacg ttagcggtta aggccaccag cggcggtaaa tcctcgcgcg gataacgctt
    63721 cgtcagttca cgagagatat ccagcccggt catatctggc aactgaatat ccagcaacac
    63781 caggtcgtat tcgcccggtt taaacatctc cagcgccgcc ttgccggtca tggcgacatc
    63841 aacgctgtta cctaattttt ccagcacaga acgcgcgaca atcacgttca gttcaatgtc
    63901 ttccaccagc agcacattca gcgccggtaa aggcatatcg tcttcatcaa acgcatcatc
    63961 gacctcttct gctaccgacg gcgcgtggat cgtcaacgta aaggttgaac ctttaccctg
    64021 ttcgctggta accgtaatat cgccgcccat atttttcgcc agacgacgag aaacggccag
    64081 accaataccg gtgccggtgg caggtttacc gccatgactg tctttcacct gataatacat
    64141 ggcgaagatt ttatccagct catcctgcgg aatgccgata ccagaatctt ccacttcaaa
    64201 atgcagcata tcgccttcat cgtagcgcac gcgcacggta acctggcctt gctgggtgaa
    64261 tttgacggcg ttactgatga ggttccacag gatctgccgt aaacgcgtcc cgtcggtaat
    64321 gacctgatgc ggtaatggca gcgtcggctc cagattaaag cgcaatcctt tttgttgcgc
    64381 ctgcaaggcg gagagatttt ccagatcggc aaggaagctg gtgaaatcaa ccggctgatt
    64441 atcaagctgg accttgcgcc gttccatctt atccatgtcg ataatatcgt tgaagatatt
    64501 ccccagcgtg acggccgaaa catggatagt tttgagatat ttttcctgct cggcggtgag
    64561 ttcggtatcc agtaggatgc ggctcaggcc gacgataccg ttcagcggtg tacgcaattc
    64621 gtgactgatg gtggaaataa acgtcgtttt gtcgcggctg gcgcgttcaa gcgcatcctg
    64681 ataccgctta cgctcggtaa tgtcgcgacc aaagcccatc aaaccgtgac gtttacccac
    64741 gcggtcgtag tacggcactt tacgaatttc aaagcaggct ttgcgtccgt cgggataatc
    64801 cagccactgc tcataggtca gtgacacatt atgacggaac actttttcat cggtttcaat
    64861 gactttcgca gcggcttccg gtgagtaaac atcagcaggt ttcagatgaa ccagttgttt
    64921 ttcgcttttt ccggtcagca gctccatcgc gcggttacag ccggaaaact ctttatcttc
    64981 gttacgataa aaaaccaggt cgggtgaagc atcaaggaag gaacgtaaga atgaggattg
    65041 ctgctcgagc tgaatttgtg tctcttcgcg ctctttgatt tcaattttca gttggccgaa
    65101 ggtttcctgc agttctgctt ccgctttttc acgaacggca atttcctgat ttagctgggc
    65161 aatattatct tttaactgaa cgttgaggct caaatcgcgc tcgcgcatct cctccagttt
    65221 ttgcaccagc cgtgacagac gttgtcgtga ctcctccagt tgctcgacga ccaccgatag
    65281 aaagtagacc gcccacggcg taatcagcaa accaaagaag atagaacgaa taacatcaat
    65341 gctttcgacc tgaccatgca gcaccatggt taccgccatt tgtaccacaa tggcaagaac
    65401 gacgagggcc agcgccagca acattgagaa gcgcaccaga cctaacttca tcatcaggtc
    65461 aacataatac tgcgccagca gacgaatttg cttcattagg gaattccttc acgacaacct
    65521 gccccaataa tacccaattc tgacagttac gttataaatt gtgtgagaaa tgcggaattc
    65581 ccctcaatga cctactccgt aggaggtagc cacggacgtc ccagcgccga tccctgtacg
    65641 ccatgttcat tcagatagcg atccagctcg accattcccg tccatcgatt ttcgcaccac
    65701 agcggagcaa gcagcgtcgg gcgacgggcg ctggcggaaa tgcggtggta gatcacttcc
    65761 ggtggcgtat ggcgaatcat ttctccggca gtgagtgtgt aatcctccag ttcaataccg
    65821 ttcaaacgcc ccgcttccca ggctttcgcc ataatgctgc ctttcacaat atgcagcgga
    65881 tgcagcttta tgccgtccac gccggtttca accacgcgct ccagcgtttg caaacattca
    65941 gcctgccctt cgccaggcag gccgacaatt aagtgggaac acaccttcag cccgcgctga
    66001 cgtgccagct gggttgtacg ctgataacag gcaaaatcat ggccgcggtt gatgcgatgc
    66061 agtgttttgt cgtgggcggt ttgtagcccc agctccagcc acacttcgta accctggtcc
    66121 ttatattcgc aaagcagatc cagcaccgca tccggcacgc agtccgggcg ggtaccaaca
    66181 cacaaaccga caatattggc ctggctcacc gcctgctgat acatagaacg cagcacctga
    66241 acttccgcaa aggtgctggt atacgcctga aagtaggcca gatagcgttt agcgcggtta
    66301 actaaattcg cctggtgcgc cagttgctcg gcaatggaac gatgctgctg cgcttcatcg
    66361 gcaaacgagg ccacattaca gaatgtgcag ccaccacgcc cgatggtacc gtcacggtta
    66421 gggcagctaa aaccgccgtg cagcgtcagc ttatgcacct tttgcccata acgacgggtg
    66481 agatcaccac caaacatatt gactaatttc tgtaactgca taatctgata gaccgcgcct
    66541 tgaaaagagg ccaaagcctg ccatttttag ctcaattcgg cgatgacctg gatcaatcgt
    66601 cccggcctgc ttttatcaac tgcataatca atcaaaatta ccgaaatttc atgcataatc
    66661 acataaatca cttttgctta tcttgtgtca gattttttta tctccttatg gattttaggc
    66721 aaaaacagta gcatgaaacg ccattaccaa ttaaggcagt ataaaatgct ggttttctcg
    66781 tcagttcaag gcaggataag ggttaacacg cctttatgac agtcaggaat tggctgtttc
    66841 tctaacgacg ctccttttag ccttaattag aaaatctatt ttaatttcag tcaattaata
    66901 aagaattttg cgctaaagca catttctgta ccaataagct tgccatttga cctgtatcag
    66961 ctttcccgat aagttggaaa tccgctggaa gctttctgga tgagcagcct gctcatcata
    67021 tttatgcagt aattgagatc ccctcttcac cgtattaacc gatgcgaaaa ggacaacaag
    67081 ggggcgaatg cgaggcgcgc gtatgacacg caaaccccgt cgccacgctc tttctgtgcc
    67141 cgtgcgcagc ggttcggaag tggggttccc gcagagcctg ggggaggttc acgatatgtt
    67201 gtacgataaa tcccttgaga gggataactg tggtttcggc ctgatcgccc acatagaagg
    67261 cgaacctagc cacaaggtag tgcgtactgc aatacacgca ctggcccgca tgcagcaccg
    67321 tggcgcgatt ctcgccgatg gtaaaaccgg tgacggttgc ggcttgctgt tacaaaaacc
    67381 ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg cgtttagcga aaaactacgc
    67441 cgtcgggatg ctattcctga ataaagatcc tgaactcgcc gctgccgcac gccgcatcgt
    67501 tgaagaagaa ctgcaacgcg aaaccttgtc gattgtgggc tggcgtgatg tccccactaa
    67561 cgaaggcgtg ctgggtgaaa tcgccctctc ctctctgcca cgcattgagc aaatttttgt
    67621 gaacgccccg gcaggctggc gtccacgcga tatggagcgc cgtctgttta tcgcccgccg
    67681 ccgcattgaa aagcgtctcg aagccgacaa agacttctac gtctgtagcc tgtcgaatct
    67741 ggtgaacatc tataaaggtc tgtgtatgcc ggcggatctg ccgcgctttt atctggatct
    67801 tgcggacctg cgtctggaat cggccatttg cctgttccac cagcgcttct ccactaacac
    67861 cgtaccgcgc tggccgctgg cgcagccgtt ccgctatctg gcgcataacg gtgaaatcaa
    67921 caccattacc ggtaaccgtc agtgggcgcg tgcgcgtacc tataaattcc agacgccgct
    67981 tatccctgac ctgcacgacg ccgcaccgtt cgtcaacgaa accggctctg actccagttc
    68041 gatggataac atgctggaac tgctgctggc aggcgggatg gatatcatcc gcgctatgcg
    68101 tctgttagtg ccgcccgcct ggcagaacaa cccggatatg gacccggaac tgcgtgcctt
    68161 ctttgacttt aactccatgc atatggagcc gtgggatggc ccggcgggca tcgtgatgtc
    68221 tgacggtcgt tttgccgcct gtaacctcga ccgtaacggt ctgcgtccgg cgcgctacgt
    68281 catcaccaaa gataagctca tcacctgcgc ctctgaagtc ggtatctggg attatcagcc
    68341 tgacgaagtg gtcgaaaaag gccgcgtcgg gccaggcgaa ctgatggtta tcgacacccg
    68401 cagtgggcgt attctgcact cggcagaaac cgatgacgat ctgaaaagcc gccatccata
    68461 taaagagtgg atggagaaaa acgtccgccg actggtaccg tttgaagatc tgcccgatga
    68521 agaagtgggt agccgcgaac tggacgacga cacacttgcc agctaccaga aacagtttaa
    68581 ctacagcgcg gaagagctgg actccgtaat tcgcgtactg ggcgaaaacg gtcaggaagc
    68641 ggtcggttcg atgggcgacg acaccccatt cgccgtgctc tccagtcagc cgcgcattat
    68701 ttacgactac ttccgccagc agtttgccca ggtgactaac ccgccaatcg acccgctgcg
    68761 tgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt gaaatgaacg tcttttgcga
    68821 agcagagggc caggcgcacc gtttaagctt taaatcgccg attctgctct actccgattt
    68881 caaacagctc acgacgatga aagaggagca ctaccgcgca gatacgctgg atatcacctt
    68941 tgacgtcacc aaaaccacgc tcgaagcgac ggtcaaagag ctgtgcgaca aagccgaaaa
    69001 aatggtacgt agcggcaccg tgctgctggt gctctccgac cggaatatcg cgaaagatcg
    69061 cctgccggtt ccggctccga tggcggttgg cgcgatccag acccgtctgg tcgatcaaag
    69121 cctgcgttgc gatgccaaca tcatcgtcga aaccgccagc gcccgcgatc cgcaccattt
    69181 cgccgtgttg ctgggcttcg gtgcgacggc tatttatccg taccttgcct atgaaacgct
    69241 gggccgcctg gtagacaccc acgcgattgc taaagattat cgtaccgtga tgctcaacta
    69301 ccgtaacggc atcaacaaag gcttgtacaa aatcatgtcc aaaatgggca tctccaccat
    69361 cgcctcttac cgctgctcga aactgtttga agcggtcggt ctgcacgatg atgtagtggg
    69421 cctgtgcttc cagggggcgg tcagccgcat tgggggggcg agctttgaag acttccagca
    69481 ggatctgctg aatctgtcga aacgtgcctg gctggcgcgc aagcccatca gccagggcgg
    69541 cctgctgaaa tacgtccacg gcggcgaata ccacgcctac aacccggacg tggtgcgcac
    69601 gctgcaacag gcggtacaga gcggcgagta tagcgactat caggaatacg cgaagctggt
    69661 taatgagcgt ccggcaacca cgctgcgcga tctgctggca attacgccgg gtgaaaacgc
    69721 ggtcaacatt gccgatgttg aaccggcaag cgaactgttt aaacgcttcg ataccgcggc
    69781 gatgtctatc ggcgcgttaa gcccggaagc tcacgaggcg ctggcggaag cgatgaacag
    69841 catcggcggt aactctaact ccggtgaagg tggtgaagat ccggcgcgct acggcaccaa
    69901 caaagtgtcg cgcatcaagc aggtggcgtc cggtcgcttc ggtgttactc cggcgtacct
    69961 ggtcaatgct gacgtcattc agattaaagt cgcccagggc gcgaagccgg gcgaaggcgg
    70021 tcagttgccg ggtgataaag tcactcctta cattgccaaa ctgcgctatt cggtgccggg
    70081 cgtgacgctg atctccccgc cgccgcacca cgatatctac tctatcgagg acttagcgca
    70141 gctcattttc gacctcaagc aggttaaccc gaaagcgatg atctccgtga agctggtttc
    70201 cgaaccgggc gttggcacca tcgccaccgg tgtggcgaaa gcttatgccg atttaatcac
    70261 catcgcaggc tatgacggcg gcaccggcgc aagtccgctt tcatcggtga aatacgcagg
    70321 ctgtccgtgg gagctggggc ttgttgaaac ccaacaggcg ctggttgcca atggtctgcg
    70381 tcataagatc cgtttgcagg ttgatggcgg cctgaaaacc ggcgttgata tcatcaaagc
    70441 ggcgattctc ggcgcagaaa gcttcggctt cggcactggc ccaatggtcg cgctcggctg
    70501 taaatatctg cgtatttgcc atctgaacaa ctgcgcgacg ggtgttgcaa ctcaggatga
    70561 caaactgcgt aagaatcact atcacggcct gccattcaag gtgacgaatt actttgagtt
    70621 tatcgcccgt gaaacacgcg agctgatggc acagcttggc gtaacacgtc tggtggatct
    70681 gattggtcgc accgacctgc tgaaagagct ggacgggttc accgccaaac agcagaaact
    70741 ggcgctgtca aagctgctgg agaccgccga accgcatcca ggtaaggcac tctactgcac
    70801 cgaaaacaac ccgccgtttg ataacggcct gctgaacgcg cagttgctgc aacaggcgaa
    70861 accgtttgtc gatgagcgcc agagcaaaac cttctggttc gatatccgca acaccgaccg
    70921 ctctgtcggc gcgtcgcttt caggctatat cgcccagacg catggcgatc aggggctggc
    70981 agccgatccg attaaagcgt acttcaacgg cactgcaggc cagagcttcg gcgtgtggaa
    71041 cgcgggcggc gtggaactgt acctgaccgg tgatgccaac gactatgtcg gtaaaggcat
    71101 ggcgggcggc ttaatcgcca ttcgtcctcc ggttggttcc gccttccgca gccatgaagc
    71161 aagtattatc ggcaacacct gcctgtatgg cgcgaccggt ggtcgtctgt atgccgcagg
    71221 ccgcgcgggt gaacgtttcg gcgtgcgtaa ctccggtgct atcaccgtgg tagaaggcat
    71281 tggcgacaac ggttgtgaat atatgacggg tggtatcgtc tgcattctgg gtaaaaccgg
    71341 cgttaacttc ggtgcgggca tgaccggcgg cttcgcttac gttctcgatg aaagcggcga
    71401 tttccgcaaa cgcgttaacc cggaactggt cgaggtctta agcgttgacg atctggcgat
    71461 ccatgaagag catctgcgtg gtcttatcac cgagcatgtt cagcataccg gttctcagcg
    71521 tggtgaagag attctggcga actggtcaac cttcgccact aaatttgcgc tggttaaacc
    71581 gaagtccagt gatgtcaaag cactgctggg tcatcgtagt cgtagcgcag cagagttgcg
    71641 cgtgcaggcg cagtaagggg tagcaacaat gagtcagaat gtttatcaat ttatcgacct
    71701 gcagcgcgtt gatccgccaa agaaaccgct gaagatccgc aaaattgagt ttgttgaaat
    71761 ttacgagccg ttttccgaag gccaggccaa agcgcaggct gaccgctgcc tgtcgtgcgg
    71821 caacccatac tgcgagtgga aatgcccggt acacaactac atcccgaact ggcttaagct
    71881 cgccaacgag gggcgtattt ttgaagcggc agaactgtcg catcagacca acactctgcc
    71941 ggaagtatgc ggacgtgtct gcccgcaaga ccgtctgtgc gaaggttcct gcaccctgaa
    72001 cgatgagttt ggcgcggtga ccatcggcaa cattgagcgt tatatcaatg ataaagcgtt
    72061 cgaaatgggc tggcgtccgg atatgtccgg cgtgaaacag accggtaaaa aagtggcgat
    72121 tatcggcgca ggtccggcag gtctggcgtg tgcagatgtc ctgacgcgca acggcgtaca
    72181 agcggtcgtc ttcgaccgtc acccggaaat tggcggcttg ctgaccttcg gtattccggc
    72241 tttcaaactg gaaaaagagg taatgacgcg ccgccgtgaa atcttcaccg gcatgggtat
    72301 tgaattcaaa ctcaataccg aagtgggccg cgacgtgcag ctggacgatc tgctgagtga
    72361 ttacgatgcc gtgttccttg gcgtcgggac ttatcagtca atgcgcggcg ggctggaaaa
    72421 cgaagacgcc gatggcgtgt acgcagcgct gccgttcctc atcgccaaca ccaaacagtt
    72481 aatgggcttt ggtgaaacca acgacgaacc gttcgtcagc atggaaggta aacgcgtggt
    72541 agtacttggc ggtggcgaca ctgcgatgga ctgcgtgcgc acgtctgtac gccagggagc
    72601 gaagcacgta acctgtgcct atcgtcgtga tgaagagaac atgccaggtt cccgccgcga
    72661 agtgaaaaac gcgcgggaag aaggtgttga gttcaaattc aacgtccaac cgctggggat
    72721 tgaagtgaac ggtaacggca aagtcagcgg cgtaaaaatg gtgcgtaccg aaatgggcga
    72781 accggacgcc aaaggccgtc gccgcgcgga gatcgttgca ggttccgaac atatcgttcc
    72841 ggcagatgcg gtgatcatgg cgtttggttt ccgcccacac agcatggaat ggctggcaaa
    72901 acacagcgtc gagctggatt cgcaaggccg tatcatcgct ccggaaggca acgacaacgc
    72961 tttccagacc agcaacccga aaatctttgc tggcggcgat atcgtccgtg gttccgatct
    73021 ggtggtgacc gctattgccg aaggtcgtaa agcggcagac ggcattatga actggctgga
    73081 agtttaagct ctgtcccagc gccgcctcct cagaaaagag tcggcgctga aattctcctt
    73141 tcgttctgga aagcgcctcg cataatctga agaaaaccaa tgtaatacgc acatctggat
    73201 tgttaccggc agatgcccgg cgtatagctg attccattat tcaacgcagc gccatataaa
    73261 gggacagata atggaatcgc tctcagagag aacctcaaca ggctaccagc aaatccacga
    73321 cggcattatt catctggtcg atagcgcccg aacggaaacg gtacgtagcg ttaacgcgtt
    73381 aatgaccgcg acgtactggg aaattggccg acgaattgtc gaatttgaac aaggtggcga
    73441 ggccagggct gcgtatggtg cgcagctaat caagcgacta tcaaaggatt taagtctaag
    73501 gtataagcgt gggttctctg caaaaaactt acgccaaatg aggctttttt acctcttttt
    73561 tcaacatgtt gaaattcgcc agacagtgtc tggcgaatta acaccattgg gaattccgca
    73621 gacaccatct gcagaattcc catcggcaaa gatttggcag acgctgtctg ccaaatcttt
    73681 ccccctgccc tggtccacct acgtccgttt actttccgtt aaaaacgctg acgcccgcag
    73741 cttttatgaa aaagagacgc tccgctgtgg ctggtctgtt cgccagctag aacggcaaat
    73801 tgcgacccaa ttttatgagc gaacactgct gtcacatgac aaatcagcca tgctgcaaca
    73861 acacgctcct gccgagacgc atattcttcc gcaacaggcg atacgcgatc cctttgtgct
    73921 cgaatttctg gaattgaaag atgaatactc agaatccgat tttgaggagg cgctgatcaa
    73981 ccacctgatg gatttcatgc tggaacttgg ggatgatttt gcctttgttg gtcggcagcg
    74041 aaggttacgc attgatgaca actggtttcg tgtcgatctg ctgtttttcc accgccgttt
    74101 acgctgcctg ctaatcgtcg atctaaaagt gggcaaattc agctatagcg atgccggaca
    74161 gatgaatatg tatctcaact acgccaaaga gcactggacg ctaccggatg aaaatccgcc
    74221 catcggtctg gttctctgtg cagagaaagg agtcggagaa gcgcattatg cactggctgg
    74281 tttgcctaac accgttctgg cgagtgaata taagatgcaa ctacctgatg aaaaacgact
    74341 cgcggatgaa ctcgttcgaa cacaggcggt gctagaagaa ggctatagac tccgttaatt
    74401 tcagcggttt cgaattctgc agacactgtc tgcagaatct ttaaaacgaa ctaagtttta
    74461 agccattaaa tcagccttaa ccttcaccac aaccttttta atctcaccag gttcgccgac
    74521 aacgcatcct ggtttatgcg gttctcccgg cataaacacg gcgaacattc ccggttttaa
    74581 gttgatggtt tgctcgtttt caatggcgct gcaaagctgg taatcatctt catggtggaa
    74641 ctcttcacac tggcgcgcag tgcctgccgt gccaaacaga atccgttcct caccttttaa
    74701 taacagctgg atatcaatgt attgctcgtg caattccgct tttttctcga cgggcgattg
    74761 agtgttaaac gtcatgacat tcataaaaat attgtcgccc tgtaattcgt aacgacccgg
    74821 cgctttttct tgcggtctgg cagctaatgc cagcgttaac gcgtcctgta acgcaggatg
    74881 taacccagca gacggtaatg actgtacttc acccatcatc ataatttttc tccctgggcc
    74941 aacagcgcag ccccaagtaa acctgcatca tggcggtagt gcgccgccag taaatcaaca
    75001 tgaaatgccg ctggctcctg cgccagatac gtttccacca gcgccagata cccttctgcc
    75061 agaccaacgc tgccaccgac caccacgcac tgacaatcag ttgtggcttt aatatcagcg
    75121 atcagccttg ccagcacatg tgcggagcgg tgaatcagct gctgcgcctg ctcgtcgccc
    75181 tgcccggcgc gcatgaaaat agttttcgca tccgcgccag ccaactcccc ctgcgctgcc
    75241 gctgcaatac cgcgaccaga agcaatcgct tccacgcaac ctgtgcgtcc acagccgcag
    75301 actgggccgt gtggatcggc tagcgtatgc ccgatgtgcc ccgccagacc gccagggccg
    75361 gtaagcagtt tgccgccgct cactacaccg ccgccgacgc cagtggaaac ggtgataaac
    75421 accatctcgg ttacatcgcc ttccagcgcc tgatactctg cccatgctgc ggcctgcgcg
    75481 tcgttaatgg caatggtcgg caaattggta agttgttcca gcgttttgac taacggaaag
    75541 tgtaacaatc caccaagatt atgcggattc agcgccagca agctgccgtc gcgaataatc
    75601 ccggttgaag cgatggcaac ccgctgcgca tgagcttgca acggagagac taatgcggat
    75661 aaggcatcac gcagtgcctg cggagtctgg ctggcaggcg taggaagttc tcggcgatcg
    75721 cggatctgcc cgtcagcgcc aatcagcgcg gcggcaagtt tagtaccgcc gatatcaatc
    75781 gccagcgtgg tcatagcacc gcctttttca tcgctgtgtt gtaccactga caaatgtgtt
    75841 caagacgcgt aatggcagaa ccgaccgtca ccgcccacgc gccgtggcgc atcgcatcag
    75901 ccgcctgagc aggcgtgttg taacgccctt cggcaatcac tcgacatccg gcgtcactca
    75961 accttttcac cagcgtcaga tccggctctt ctggcgtttc aggcgtggta tagccagaaa
    76021 gcgtagtgcc aataatttcg gctcccagct tttggcatgc caggccgtct tccggcgttg
    76081 agcagtcggt catcgccagt aaaccgtgat gatgaatacg tgccagcagc gtttcaacag
    76141 gcaccggacg cggtcggtca gtgccgtcaa tggcgataat gtcagcgccc gcctgcgcca
    76201 gcgcatcgac atcttcaata taggccgtga tgcgtaccgg agaatcctcc agatcgcgtt
    76261 tcacaatccc gataatcggc acgctcacca ccgcacgcgt ggcttgcaga tttgccacac
    76321 cttcgatgcg aatggcaaca gcgcccgcct gctctgccgc taatgccatg gcagcgacaa
    76381 tgtcgggttt atcgagcggg ctgtccggaa ccggctggca ggagacaatc aggccaccgt
    76441 tagcagcgat tttttgatcc agttgtgcaa gtaacgacat acatcttccc ttagcgaaag
    76501 gcccggtaca gagaccgggc aacaggatta acttttggtt ttgactaaat cgtttttggc
    76561 gcggccaaat ggcacggcac cgctaaatgg tttgccgtcg atagcgtcat gagtacgcaa
    76621 tgcttccggg cgcagccaac gctgaacacg ggaaggcata tccagaccaa tcagcaggat
    76681 caccacaaat gtcagactga acgagagcga tgccagcgca gtacccagat ccagacgttg
    76741 agcgatcaac gcgccgatga ttggggccag tgcgccaccc aatgcgccaa cgttataggt
    76801 aaagcccagg cccgctgcac gctggtcggt atcgaaataa ccgccaatca gtttcggtaa
    76861 gatcccggcg atcccttgtc caagcatttg ctggaagaac agtaacagac cgagcaccca
    76921 gacgtttgcg ccgccaatcg caaataccgg aataatcagc agctgcgagg ccaacagact
    76981 acaaacgtac gctttgcggg ttcccagcca gtcaccgagg aagccaccga cgcagcaccc
    77041 caccgccgcg ccaaagccac taaagaacag cacattggcg acagtatgcg ggttataagc
    77101 cagatcggtt ttcagatacg ttggcagcag cgcctgaatc ggccatgagt agaggaaagc
    77161 aaacaacacg accaccatca gcattacgcc agttggccag cgtttgcccg tactctgcac
    77221 cataaagctg ataaagattg cggcgcataa cagcccaaga acagcgacga tcgcggcatt
    77281 ttgcaggtta ccggcgaagc agaaccacag cgcagtagcc gccgccagtg tcattacgat
    77341 attggcaatg cgatgttcac cacggtagag aatatccacc attgtgcgta ctggtgcttt
    77401 acctgcgtgt ttctctttcc agtcttccgc ttccgggatg tttttacgca gccagagagc
    77461 aaagataatt ggcaaaatgc cgataaagaa cagcgcacgc cagccccaga ccggaaccac
    77521 caggctgtag acctgagcgg caacgacggc ccccacagag aagcctgaaa tcagaaagcc
    77581 actggctttg ttacgcagat gttttggcca gctttcaatg acataggtgg cgctggaacc
    77641 gtattcaccc gccatcccca tgccgatgac cagacgagcg ataaacatgg tgatgtagcc
    77701 tggcgcaaag ccgcaggcca gcgtcccggc cgagaagaga acgatgctgg tgaccattgc
    77761 cagacgacgc ccgtagcggt cacccatagc gccgagcatc aggccgccga accagcgaga
    77821 gataaaggct gcggagatca gacttgccgc ctgcaccgtc gtcagcccga attcaccttg
    77881 tacttcggtg agtaccaggg cgattaaaac gaaatcaaaa ccgtcaagca gatatcccaa
    77941 ccaggcagcg gaaaatgccc gccattgtgc acgattgaga tggcgatacc acgggatatt
    78001 ctgggttgta gtactcattg tgagtctccc gcggtgggcg atgcccacac gctttggtat
    78061 gaaaattgta gggtacagat gcgtttattt cccctcaccc ggtaggggcg agcgagggga
    78121 aacaactcac ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata
    78181 tttttcatct accggaccaa acggtttgcg gcacagcggc acagacacga catccatata
    78241 atgcagaaca gttttcaggc cgcggaatac gcccgttttg atcagtaaat caatgacttt
    78301 attgcactca gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac
    78361 gatcccctga tagcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc
    78421 tgccagcaag ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg
    78481 ttcacgacgg atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac
    78541 gccaggcaac gtaacaagtg tgttgatctg atccagggtc agttttaccc cactcagggc
    78601 tggaatgttg tacaccacca tcggcaaccc atccgctgaa tcaataattg cccgatagtg
    78661 atcgcagtgt tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc
    78721 gaagccataa cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc
    78781 gacgtgggcg atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg
    78841 ttcacgctcg gaaaggcttt gtacaaaggc ttcgccggtc gaaccaccca cgtataaacc
    78901 gtcaatgccc tgctgaatat tgaactgaac caggcggcgc agactcgctt tatccagtgc
    78961 ttgttgttga tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat
    79021 aaatacctct gaagtgatgc ttgtctgata aacgatatac ctttatacct gttataccag
    79081 atcaattaag caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc
    79141 attatttctt tttgttggtg gtctgaccga aggcgtgcca ggtagcagag acgctgttga
    79201 gatgcgattg caacgcacga tcggcttcgt caggatcatg acgccggatc gcgtcaacga
    79261 tcgcaatatg ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg
    79321 ttggacgtgc ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt
    79381 tacctgggat ctccgccagc acgcggtgga aatcaacgtc tgagcgaatg aatgccgcgt
    79441 tgttatccag cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat
    79501 cggtggcgtg ttcagccgca tagcgcacca ggctggattc aaagaacaga cgtaattgtt
    79561 cgaaatgggc aatcccacca ggatgagaaa ggaaatcttt cgccatgccg gaaagctcac
    79621 cgatgatggt gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca
    79681 ccagaccttt gcgttttaac gctgccagcg cttcacgcac cgaaggacgc ccgacgttaa
    79741 agaacgccat cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc
    79801 ggatcatctg ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc
    79861 ggctacgcaa gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt
    79921 tcataaggcc cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag
    79981 gattgaaagt aggggaaaat ggcagggttt tctctttgtg cctcatcatt accataatta
    80041 acggaataat taactattgc gaaaaattaa tgtaacgcag ataaaaacat cccgattgaa
    80101 ttatttacaa gactattcac gagcattatg aatattatga a
//
LOCUS       DAZUCE010000003        66003 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZUCE010000003 DAZUCE010000000
VERSION     DAZUCE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.003"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(244..320)
                     /locus_tag="ACHY03_000152"
     tRNA            complement(244..320)
                     /locus_tag="ACHY03_000152"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(284..286),aa:Arg,seq:acg)
     gene            complement(324..416)
                     /locus_tag="ACHY03_000153"
     tRNA            complement(324..416)
                     /locus_tag="ACHY03_000153"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(380..382),aa:Ser,seq:gct)
     gene            complement(733..918)
                     /gene="csrA"
                     /locus_tag="ACHY03_000154"
     CDS             complement(733..918)
                     /gene="csrA"
                     /locus_tag="ACHY03_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFS3903443.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1153..3783)
                     /gene="alaS"
                     /locus_tag="ACHY03_000155"
     CDS             complement(1153..3783)
                     /gene="alaS"
                     /locus_tag="ACHY03_000155"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFS3903444.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3911..4411)
                     /gene="recX"
                     /locus_tag="ACHY03_000156"
     CDS             complement(3911..4411)
                     /gene="recX"
                     /locus_tag="ACHY03_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFS3903445.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4654..5715)
                     /gene="recA"
                     /locus_tag="ACHY03_000157"
     CDS             complement(4654..5715)
                     /gene="recA"
                     /locus_tag="ACHY03_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFS3903446.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5795..6292)
                     /gene="pncC"
                     /locus_tag="ACHY03_000158"
     CDS             complement(5795..6292)
                     /gene="pncC"
                     /locus_tag="ACHY03_000158"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFS3903447.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6437..7522)
                     /gene="mltB"
                     /locus_tag="ACHY03_000159"
     CDS             complement(6437..7522)
                     /gene="mltB"
                     /locus_tag="ACHY03_000159"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFS3903448.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7778..8341
                     /gene="srlA"
                     /locus_tag="ACHY03_000160"
                     /gene_synonym="gutA"
     CDS             7778..8341
                     /gene="srlA"
                     /locus_tag="ACHY03_000160"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFS3903449.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8338..9297
                     /gene="srlE"
                     /locus_tag="ACHY03_000161"
                     /gene_synonym="gutE"
     CDS             8338..9297
                     /gene="srlE"
                     /locus_tag="ACHY03_000161"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFS3903450.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGIAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9308..9679
                     /gene="srlB"
                     /locus_tag="ACHY03_000162"
                     /gene_synonym="gutB"
     CDS             9308..9679
                     /gene="srlB"
                     /locus_tag="ACHY03_000162"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFS3903451.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9683..10462
                     /gene="srlD"
                     /locus_tag="ACHY03_000163"
     CDS             9683..10462
                     /gene="srlD"
                     /locus_tag="ACHY03_000163"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFS3903452.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10568..10927
                     /gene="gutM"
                     /locus_tag="ACHY03_000164"
     CDS             10568..10927
                     /gene="gutM"
                     /locus_tag="ACHY03_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFS3903453.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10994..11767
                     /gene="srlR"
                     /locus_tag="ACHY03_000165"
                     /gene_synonym="gutR"
     CDS             10994..11767
                     /gene="srlR"
                     /locus_tag="ACHY03_000165"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFS3903454.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11760..12725
                     /gene="gutQ"
                     /locus_tag="ACHY03_000166"
     CDS             11760..12725
                     /gene="gutQ"
                     /locus_tag="ACHY03_000166"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFS3903455.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLVGAINLQDFYQAGII"
     gene            complement(12722..14236)
                     /gene="norR"
                     /locus_tag="ACHY03_000167"
     CDS             complement(12722..14236)
                     /gene="norR"
                     /locus_tag="ACHY03_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFS3903456.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14423..15862
                     /gene="norV"
                     /locus_tag="ACHY03_000168"
     CDS             14423..15862
                     /gene="norV"
                     /locus_tag="ACHY03_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFS3903457.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15859..16992
                     /gene="norW"
                     /locus_tag="ACHY03_000169"
     CDS             15859..16992
                     /gene="norW"
                     /locus_tag="ACHY03_000169"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFS3903458.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLLLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPEAALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTESQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPV"
     gene            complement(17412..19664)
                     /gene="hypF"
                     /locus_tag="ACHY03_000170"
     CDS             complement(17412..19664)
                     /gene="hypF"
                     /locus_tag="ACHY03_000170"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFS3903459.1"
                     /translation="MSKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHHWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRVFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19817..20344)
                     /gene="hydN"
                     /locus_tag="ACHY03_000171"
     CDS             complement(19817..20344)
                     /gene="hydN"
                     /locus_tag="ACHY03_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFS3903460.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20493..21503)
                     /gene="ascG"
                     /locus_tag="ACHY03_000172"
     CDS             complement(20493..21503)
                     /gene="ascG"
                     /locus_tag="ACHY03_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFS3903461.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVEMLLERGVKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEVIGRLIFMLDGGAYTPPKTFSGKLICRDSLKAL
                     PR"
     gene            21763..23220
                     /gene="ascF"
                     /locus_tag="ACHY03_000173"
     CDS             21763..23220
                     /gene="ascF"
                     /locus_tag="ACHY03_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFS3903462.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDS
                     PMLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAETRKHQSAQPTVAKEVSLN"
     gene            23229..24653
                     /gene="ascB"
                     /locus_tag="ACHY03_000174"
     CDS             23229..24653
                     /gene="ascB"
                     /locus_tag="ACHY03_000174"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFS3903463.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNPQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMYLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDSLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24812..25282)
                     /gene="hycI"
                     /locus_tag="ACHY03_000175"
     CDS             complement(24812..25282)
                     /gene="hycI"
                     /locus_tag="ACHY03_000175"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFS3903464.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(25275..25685)
                     /gene="hycH"
                     /locus_tag="ACHY03_000176"
     CDS             complement(25275..25685)
                     /gene="hycH"
                     /locus_tag="ACHY03_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFS3903465.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25682..26449)
                     /gene="hycG"
                     /locus_tag="ACHY03_000177"
     CDS             complement(25682..26449)
                     /gene="hycG"
                     /locus_tag="ACHY03_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFS3903466.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPSEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26449..26991)
                     /gene="hycF"
                     /locus_tag="ACHY03_000178"
     CDS             complement(26449..26991)
                     /gene="hycF"
                     /locus_tag="ACHY03_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFS3903467.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(27001..28710)
                     /gene="hycE"
                     /locus_tag="ACHY03_000179"
     CDS             complement(27001..28710)
                     /gene="hycE"
                     /locus_tag="ACHY03_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFS3903468.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQSGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28728..29651)
                     /gene="hycD"
                     /locus_tag="ACHY03_000180"
     CDS             complement(28728..29651)
                     /gene="hycD"
                     /locus_tag="ACHY03_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFS3903469.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIIKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29654..31480)
                     /gene="hycC"
                     /locus_tag="ACHY03_000181"
     CDS             complement(29654..31480)
                     /gene="hycC"
                     /locus_tag="ACHY03_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFS3903470.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLSVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIVLLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31477..32088)
                     /gene="hycB"
                     /locus_tag="ACHY03_000182"
     CDS             complement(31477..32088)
                     /gene="hycB"
                     /locus_tag="ACHY03_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFS3903471.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(32213..32674)
                     /gene="hycA"
                     /locus_tag="ACHY03_000183"
     CDS             complement(32213..32674)
                     /gene="hycA"
                     /locus_tag="ACHY03_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFS3903472.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32886..33236
                     /gene="hypA"
                     /locus_tag="ACHY03_000184"
     CDS             32886..33236
                     /gene="hypA"
                     /locus_tag="ACHY03_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFS3903473.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            33240..34112
                     /gene="hypB"
                     /locus_tag="ACHY03_000185"
     CDS             33240..34112
                     /gene="hypB"
                     /locus_tag="ACHY03_000185"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFS3903474.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            34103..34375
                     /gene="hypC"
                     /locus_tag="ACHY03_000186"
     CDS             34103..34375
                     /gene="hypC"
                     /locus_tag="ACHY03_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFS3903475.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            34375..35496
                     /gene="hypD"
                     /locus_tag="ACHY03_000187"
     CDS             34375..35496
                     /gene="hypD"
                     /locus_tag="ACHY03_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFS3903476.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     QRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35493..36503
                     /gene="hypE"
                     /locus_tag="ACHY03_000188"
     CDS             35493..36503
                     /gene="hypE"
                     /locus_tag="ACHY03_000188"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFS3903477.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36577..38655
                     /gene="flhA"
                     /locus_tag="ACHY03_000189"
     CDS             36577..38655
                     /gene="flhA"
                     /locus_tag="ACHY03_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFS3903478.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(38692..39045)
                     /gene="ygbA"
                     /locus_tag="ACHY03_000190"
     CDS             complement(38692..39045)
                     /gene="ygbA"
                     /locus_tag="ACHY03_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFS3903479.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTARHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(39077..39256)
                     /locus_tag="ACHY03_000191"
     CDS             complement(39077..39256)
                     /locus_tag="ACHY03_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3903480.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     EPFFFRVIVPFKIKL"
     gene            39332..41893
                     /gene="mutS"
                     /locus_tag="ACHY03_000192"
     CDS             39332..41893
                     /gene="mutS"
                     /locus_tag="ACHY03_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFS3903481.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGVENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLDRAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            41999..42655
                     /gene="pphB"
                     /locus_tag="ACHY03_000193"
     CDS             41999..42655
                     /gene="pphB"
                     /locus_tag="ACHY03_000193"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFS3903482.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQKEAISLLLKFHYLPHIIEITNDIIKYVIAHADYPGDEYQFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(42696..42932)
                     /locus_tag="ACHY03_000194"
     CDS             complement(42696..42932)
                     /locus_tag="ACHY03_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFS3903483.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(42943..44370)
                     /locus_tag="ACHY03_000195"
     CDS             complement(42943..44370)
                     /locus_tag="ACHY03_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFS3903484.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(44370..44962)
                     /locus_tag="ACHY03_000196"
                     /pseudo
     CDS             complement(44370..44962)
                     /locus_tag="ACHY03_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
     gene            45109..45516
                     /locus_tag="ACHY03_000197"
     CDS             45109..45516
                     /locus_tag="ACHY03_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFS3903485.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSAEEQELFVQLVRKMMNT"
     gene            complement(45636..46628)
                     /gene="rpoS"
                     /locus_tag="ACHY03_000198"
     CDS             complement(45636..46628)
                     /gene="rpoS"
                     /locus_tag="ACHY03_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFS3903486.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(46691..47785)
                     /gene="nlpD"
                     /locus_tag="ACHY03_000199"
     CDS             complement(46691..47785)
                     /gene="nlpD"
                     /locus_tag="ACHY03_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFS3903487.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(47970..48596)
                     /gene="pcm"
                     /locus_tag="ACHY03_000200"
     CDS             complement(47970..48596)
                     /gene="pcm"
                     /locus_tag="ACHY03_000200"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFS3903488.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(48590..49351)
                     /gene="surE"
                     /locus_tag="ACHY03_000201"
     CDS             complement(48590..49351)
                     /gene="surE"
                     /locus_tag="ACHY03_000201"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFS3903489.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHNHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(49332..50381)
                     /gene="truD"
                     /locus_tag="ACHY03_000202"
     CDS             complement(49332..50381)
                     /gene="truD"
                     /locus_tag="ACHY03_000202"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFS3903490.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(50378..50857)
                     /gene="ispF"
                     /locus_tag="ACHY03_000203"
     CDS             complement(50378..50857)
                     /gene="ispF"
                     /locus_tag="ACHY03_000203"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFS3903491.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(50857..51567)
                     /gene="ispD"
                     /locus_tag="ACHY03_000204"
     CDS             complement(50857..51567)
                     /gene="ispD"
                     /locus_tag="ACHY03_000204"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFS3903492.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQIPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(51586..51897)
                     /gene="ftsB"
                     /locus_tag="ACHY03_000205"
     CDS             complement(51586..51897)
                     /gene="ftsB"
                     /locus_tag="ACHY03_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFS3903493.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(52091..52414)
                     /gene="ygbE"
                     /locus_tag="ACHY03_000206"
     CDS             complement(52091..52414)
                     /gene="ygbE"
                     /locus_tag="ACHY03_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFS3903494.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(52464..53069)
                     /gene="cysC"
                     /locus_tag="ACHY03_000207"
     CDS             complement(52464..53069)
                     /gene="cysC"
                     /locus_tag="ACHY03_000207"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFS3903495.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(53069..54496)
                     /gene="cysN"
                     /locus_tag="ACHY03_000208"
     CDS             complement(53069..54496)
                     /gene="cysN"
                     /locus_tag="ACHY03_000208"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFS3903496.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(54498..55406)
                     /gene="cysD"
                     /locus_tag="ACHY03_000209"
     CDS             complement(54498..55406)
                     /gene="cysD"
                     /locus_tag="ACHY03_000209"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFS3903497.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            55658..56695
                     /gene="iap"
                     /locus_tag="ACHY03_000210"
     CDS             55658..56695
                     /gene="iap"
                     /locus_tag="ACHY03_000210"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFS3903498.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   56777..57171
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=56777..56805
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(57268..57561)
                     /gene="cas2e"
                     /locus_tag="ACHY03_000211"
     CDS             complement(57268..57561)
                     /gene="cas2e"
                     /locus_tag="ACHY03_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFS3903499.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(57558..58481)
                     /gene="cas1e"
                     /locus_tag="ACHY03_000212"
     CDS             complement(57558..58481)
                     /gene="cas1e"
                     /locus_tag="ACHY03_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFS3903500.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARHPAESDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(58478..59128)
                     /gene="cas6e"
                     /locus_tag="ACHY03_000213"
     CDS             complement(58478..59128)
                     /gene="cas6e"
                     /locus_tag="ACHY03_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFS3903501.1"
                     /translation="MYLSRITLHTAQLLPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGRDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(59110..59854)
                     /gene="cas5e"
                     /locus_tag="ACHY03_000214"
                     /pseudo
     CDS             complement(59110..59854)
                     /gene="cas5e"
                     /locus_tag="ACHY03_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
     gene            complement(59865..60920)
                     /gene="cas7e"
                     /locus_tag="ACHY03_000215"
     CDS             complement(59865..60920)
                     /gene="cas7e"
                     /locus_tag="ACHY03_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFS3903502.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGCMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(60932..61471)
                     /gene="casB"
                     /locus_tag="ACHY03_000216"
                     /gene_synonym="cse2"
     CDS             complement(60932..61471)
                     /gene="casB"
                     /locus_tag="ACHY03_000216"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFS3903503.1"
                     /translation="MSIVKDGHKATLRKWHEELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVAANVKAIDERQPFAAQLAAVMSEGRFTCL
                     SAVKTPDELLRQLRRAVKLLNGSVNLISLADDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            complement(61468..63030)
                     /gene="casA"
                     /locus_tag="ACHY03_000217"
                     /gene_synonym="cse1"
     CDS             complement(61468..63030)
                     /gene="casA"
                     /locus_tag="ACHY03_000217"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFS3903504.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQSSFAPKDYRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFEVLMGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCSHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQAPLWRKLWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQDDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHRFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFSRQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(63128..65827)
                     /locus_tag="ACHY03_000218"
     CDS             complement(63128..65827)
                     /locus_tag="ACHY03_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFS3903505.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRDEKEGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLHP
                     QLKVPEGARKNYEKISHSTLGYWLWHHYLSECEELLPASSLSTRKLKRVIAMWMPMTT
                     GHHGRPPDRIDELDNFLPEDKVAARDFLLEIKALCPLIEIPTFWDNDEGVELLKQLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGV
                     NTLFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFANSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIEMETLARFGKE
                     DGSLRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFKPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIAGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRQWCIEQRQDPEMANVILVTDDESCGYSAREGLIGKVD"
BASE COUNT        15597 a        18163 c        16936 g        15307 t
ORIGIN      
        1 ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcatcggg aaacttactt
       61 tactgcagat tttttgatac cgctactaaa gctgtatcaa gtaagagatg gtgcatccgg
      121 gaggattact cgccttgcgg ctcgcccttc gggccgttgc taaagcaacg ttatcctccc
      181 tggtgcctgc ggttagtacc gccacctctt ccataccttc atcgctgaag gttacggaga
      241 atatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta
      301 tccagctgag ctacggatgc aaatggcggt gaggcgggga ttcgaacccc ggatgcagct
      361 tttgaccgca tactccctta gcaggggagc gccttcagcc tctcggccac ctcaccacac
      421 gcctcttacg agtgcttcga agaacttgtt tatcgctcat cgtcgctgcg tggcgcacat
      481 attactttct gagacttata agtcaaacaa tttttcccac acttttatcg tttgcacact
      541 tcacgctcaa ttagtctgta aaaacgtcaa aaagagtgtt ttatcaacag aaaaacggag
      601 gtctgacaga tagcagtaat gtaaaaaata tggagactta agttgaatga acgggagtaa
      661 agcgaaaaga ctatagagtg aaggagaaat tttgagggtg cgtctcaccg ataaagatga
      721 gacgcggaaa gattagtaac tggactgctg ggatttttca gcctggatac gctggtagat
      781 ctcttcacgg tgaacagaaa cttccttcgg ggcatttacg ccaatacgta cctggttgcc
      841 ctttacccct aaaactgtca cggtgacctc atccccaatc atgagggtct caccaactcg
      901 acgagtcaga atcagcattc tttgctcctt gaaagattaa aagagtcggg tctctctgta
      961 tcccggcatt atccatcata taacgccaaa aagtaagcaa tgacaaacac attacatcta
     1021 agcagtcatg gcattacatt ctgttaaacc taagtttagc cgatatacac aacttcaacc
     1081 tgactttatc gttgtcgata gcgttaatgc gaatgccgtg aagcgagtcc acggcattgc
     1141 ctgacgctta tattattgca atttcgcgct gacccagcct ttcacactgg ctaacgctgc
     1201 aggtaaggcc gcagcatccg taccaccggc ttgcgccatg tcaggacgtc caccaccctt
     1261 gccgcccacc tgctgagcga ccataccaat cagttcccct gctttcacac gatctgtgac
     1321 gtccttagat acgcctgcaa tcagagaaac cttaccttcg gctaccgttg ccagcacgat
     1381 aattgtcgac cccagctgat tttttaaatc gtcaaccatg gtacgcaaca ttttcggctc
     1441 aacaccgcta agctcgctaa ccaacagctt aacaccatta acatcaattg ccttactgga
     1501 aagatttgcg ctctcctgtg cggcagcttg ttctttaagc tgttgtagtt ctttttctaa
     1561 ctgacgcgta cgttccagta ctgagcgcac tttatcagcc agattattgc tatcgccttt
     1621 cagcagatgt gcgacttcgc ttaagcgatc gctgtctgca tgaacggtgg cgatagcacc
     1681 ttctccggtt accgcttcga tacgacgaac gcctgcagca gtacccgatt cagagatgat
     1741 gcggaacaga ccaatatcac cagtgcggct ggcgtgagta ccgccacaca gctcggtgga
     1801 gaaatcgccc atgctcagca cgcgtacgcg ctcatcatac ttctcgccga acagcgccat
     1861 cgcacctttc gctttcgccg cttcgagatc catgatgttg gtttcgatcg gcaaattgcg
     1921 acgaatctgt gcgttcacca agtcttcgac cgcacgaatc tcttctggtt tcatcgcttc
     1981 gttgtgtgag aagtcgaagc gcagcacctt gtcgttaacc agtgaacctt tctgcgatac
     2041 atgagtaccc agaacctggc gcagcgcagc gtgcatcagg tgcgttgcgg agtgattcag
     2101 acgaatacgg gcgcgacgag cttcatcaac atcagcctgc accgcgtcgc ccactttcag
     2161 agaacccgca gcaagtttac cgatgtgacc aatcgcctgg ccgtatttct gcgtatcttc
     2221 caccgcaaag gagaagttag cgcctttcag ttcgccttta tcaccaacct gaccgccgga
     2281 ttccgcatag aatggcgttt gatccagcac gaccacagct tcctggcctg cattgatggc
     2341 atcaaccgct ttaccatcaa caaacagcgc ggtcactttg ccgttcagtt ccagatggtc
     2401 atagccttta aattcagatg cactgtcaac acggatcatt gcgttgtaat cggcaccaaa
     2461 gccgctggct tcgcgcgcgc gacgacgctg ctcttccatt gcagcttcaa aaccagcttc
     2521 gtcaactttg atgttgcgct cacgacaaac atcagccgtc aggtcaaccg ggaagccata
     2581 ggtgtcgtac agacggaaag cagtttcacc atccagcgta tcaccagaaa gttttgccag
     2641 ctcttcatcc agcaacgcca gaccgcgctc cagagtacga gcaaactgct cttcttcagt
     2701 cttcagcacc tgctcaacct gcgcctgctg gcgtttcagg tcttcacccg cagagcccat
     2761 aacgtcgatc agcggaccaa ccagtttgta gaagaaggtt tctttcgcgc cgagcatatt
     2821 accgtgacgc actgcgcgac gaatgatacg acgcagtaca taaccacggt tttcattgga
     2881 cggcattacg ccatccgcga tcaggaacgc acaagaacga atgtggtcag cgattacgcg
     2941 cagcgattta ttgctcagat cggttgcgcc agtgactttc gctaccgcct ggatcaacgt
     3001 gcggaacagg tcgatgtcat agttagagtt aacgtgttgc agcaccgcag caatacgctc
     3061 cagacccata ccggtatcta cagacggttt cggcagcggt tccatcgtgc catcggcctg
     3121 gcggttgaac tgcatgaaga cgatgttcca gatctcaatg tagcggtcgc cgtcttcttc
     3181 cggacttccc ggagggccac cccaaatgtg gtcgccgtga tcgtagaaga tttcggtgca
     3241 cgggccgcac ggaccagtgt cacccatctg ccagaagttg tcagatgcgt atggcgcacc
     3301 tttgttatcg ccgatgcgaa taatacgttc gcgcgggatc cctacttctt tttcccagat
     3361 ttcgtaggct tcgtcgtcgc tttcatagac ggtaacccac agacgctctt tcggcagggc
     3421 aaaccatttt tcgctggtca gcagttccca tgcaaactga atggcatcgt gtttgaaata
     3481 gtcgccgaag ctgaagttgc ccagcatttc gaagaaggta tggtgacgcg cggtgtaacc
     3541 gacgttttcc aggtcgttgt gtttaccacc cgcacgcacg cagcgttggg aagtggtagc
     3601 gcgggaataa ttacgcttgt cgagcccaag gaacacatcc ttgaactggt tcatcccggc
     3661 gttggtaaac aacaaagttg ggtcgttatg gggtaccagg gagctgctgg caactacctg
     3721 atgtccctta ctatggaaaa agtcgagaaa cgcctgacgg atctcagcgg tgctcttgct
     3781 cataattatc ctgaaatcaa gctaacgaaa tatcgccacc agctccagcg tgtcttaacc
     3841 gccgggctgg taactgaaaa gtgggaataa gataagtttt cttgactggg aagtaaaata
     3901 ccgtatgcgt tcagtcggca aaatttcgcc aaatatcctg gatatcttcc atcagatagc
     3961 cacgatagag cagaaaacgc tggatcttaa ctttttctga aaagacagtt ggcaaaggtt
     4021 caccatattt tcgcgtcgcc tgatcgcgcg ccagtgcgca ccagtcgatg tcacattcac
     4081 gcatcgcttt ttctgtcgct tcgcgggaaa tacctttctg attcagttcc tggcgaatac
     4141 gcgcaggtcc ataacctttg cggctacggc tggcgataaa gcgcgcaaca aatcggctgt
     4201 catcgagata gccatgttca tggcaccagg caataacgcg ctcgtaatct tctgccgtag
     4261 catcaatctc ttctgggcca tttttgccca taatcggtgc cgcgagttta cgtcgcagtt
     4321 cttgctcact gtgatcgcgc accgccagaa tgcgtaccgc acgatccaac aggcgagcat
     4381 atgccgggcg acgggacgtt gattctgtca tggcatatcc ttacaactta aaaaagcaaa
     4441 agggccgcag atgcgaccca ggttttgctg ataaacagat ttaatagtca atgagtgtgc
     4501 agaatgttcc cgcaggccca attcgttaga ggatcgaggt gagagtaatt tttcacgcca
     4561 gtgctggcct gttcccctca ccctaaccct ctccccaaag gggcgagggg accgtccgtg
     4621 cacacattat ctattatcat caataacgaa cgattaaaaa tcttcgttag tttctgctac
     4681 gccttcgcta tcatctacag agaaatccgg cgttgagttc gggttgctca gcagcaactc
     4741 acgtactttc ttctcgatct ctttcgcggt ttccgggtta tctttcagcc aggcagttgc
     4801 attcgcttta ccctgaccga tcttctcacc tttgtagctg taccacgcgc ctgctttctc
     4861 gatcagcttc tcttttacgc ccaggtcaac cagttcgccg tagaagttga taccttcgcc
     4921 gtagaggatc tggaattcag cctgtttaaa cggcgcagcg attttgttct tcaccacttt
     4981 cacgcgggtt tcgctaccca ccacgttttc gccctctttc accgcgccga tacgacggat
     5041 gtcgagacga acagaggcgt agaatttcag cgcgttacca ccggtagtgg tttccgggtt
     5101 accgaacatc acaccaattt tcatacggat ctggttgatg aagatcagca gcgtattgga
     5161 ctgcttcagg ttacccgcca gcttacgcat cgcctggctc atcatacgtg ccgcaaggcc
     5221 catgtgagag tcgccgattt cgccttcgat ttccgctttc ggcgtcagtg ccgccacgga
     5281 gtcaacgacg ataacgtcta ctgcgccaga acgcgccagg gcgtcacaga tttccagtgc
     5341 ctgctcgccg gtgtccggct gggagcacag caggttgtcg atatcgacgc ccagtttacg
     5401 tgcgtagatt gggtccagcg cgtgttcagc atcgataaac gcacaggttt taccttcacg
     5461 ctgcgctgcg gcgatcacct gcagcgtcag cgtggtttta ccggaagatt ccggtccgta
     5521 gatttcgacg atacggccca tcggcagacc acctgcccca agcgcgatat ccagtgaaag
     5581 cgaaccggta gagatggttt ccacatccat ggaacggtct tcacccaggc gcatgatgga
     5641 gcctttacca aattgtttct caatctggcc cagtgctgcc gccaacgctt tctgtttgtt
     5701 ttcgtcgata gccattttta ctcctgtcat gccgggtaat accggatagt caatatgttc
     5761 tgttgaagca attatactgt atgctcatac agtatcaagt gttttgtaga aattgttgcc
     5821 acaaggtctg caatgcatac gcagtagcct gacggcgcac cgcatcacgg tcgccgctga
     5881 agcattcccg ccgggtaatg ccttcaccgc gggcagtggc aaaagcaaac cagacggtgc
     5941 cgacaggctt ctcttcactg ccgccatccg gcccggcgat accactaata gacacggcgt
     6001 aatcagcacg agccgctttc agtgcgccta tcgccatttc caccacgacg ggttcgctca
     6061 ccgcgccatg ctgcgccagc gtctcttcac gtacgccgat catctgcgct ttggcttcgt
     6121 tactgtaggt gacaaatccg cgttcaaacc aggcggagct accggcaata tcggtaatca
     6181 ctttcgctac ccaaccgccg gtacaagact cagcagttgt tacggttgcg ccacgggctt
     6241 tcagcgcctg cccaacctgt tcgcttaact gcatcagttc actgtcagtc atcacaacct
     6301 ctgtcagtca aaaatttatg ccggacaaga tagcactttc gtcaggaaga tggtgttggc
     6361 gttgcaattt tacgaggagg attcagaaaa aagctgatta gccagaagga agctcacgcc
     6421 cccctcttgc aaatagttac tgcacgcgcg ccagcgccac ggcttgtcct aactgccaga
     6481 ccgccattgc gtaatgggtg ctgtggttat aacgggtaat ggtgtagaag ttcggcagac
     6541 cgtaccagta ctggtaaccg gtaccgacat ccagacgcag caggctggct tgttgatggt
     6601 tgcccagcgg ctgctgtggc gttaaacctg ccgcggcaag ctgcgaaatg ctgtacttgg
     6661 ttttgaagcc atttggcaag cctggagcct gaccgtttgc cattaccgcg acctgatcgc
     6721 ctttcaccca gccgtgcgct ttgaaataat tcgccacgct accgatcgca tcaaccggat
     6781 cccacaggtt gatatgcccg tcgccgctga aatctaccgc atattgtttg taagacgacg
     6841 gcataaactg tccgtagccc atcgccccgg caaaggaacc tttcagattg agcggatcgt
     6901 cctgctcgtc gcgcgccatc agcaggaagg tttccagctc gccagagaaa tactccgcgc
     6961 ggcgtgggta gttaaatgac agcgttgcca gcgcatcgag gatgcgagtt ttccccatca
     7021 cgcgccccca gcgggtttca acgccgataa tcccgacgat aatttccggc ggtacgccat
     7081 acacctgcca cgcgcgattc aacgcatctt catactgatt ccagaaaacc acaccgttct
     7141 gcacattgtc cggcgtaata aattttttgc gataacgcag ccatgcgccg ttcggacctg
     7201 atggcggttt caccgatgtc gttggtgcct ggttatccat cagccgcagt accgaatcca
     7261 gacgtttcgc ctgggagaga atttcctgca actgctgacg atcgaaaccg tgtttgttca
     7321 ccattttgtc gatgaactgc tgggcattcg ggttattggc gaagtcaccg cccatctgca
     7381 tcacattgtg ctgcggctca agcaggaagc cgccagacgg cgttccggtg gtcgtatcag
     7441 tctcagtagg ttttggcttg ctgctacagg cggcaagcaa cacaaaaagg ggaagcaatg
     7501 ttacataacg acgcttgaac atgaggggtc catttaacag attcaaccag gggcaagtat
     7561 ggtaaagcat cacgccccgc acaaggaagc ggtagtcact gcccgatacg gactttacat
     7621 aactcaactc atccccctcg ctatcctttt attcaaactt tcaaattaaa atatttatct
     7681 ttcattttgc gatcaaaata acacttttaa atctttcaat ctgattagat taggttgccg
     7741 tttggtaata aaacaataaa tcctgaagga gagaacaatg atagaaacca ttactcatgg
     7801 tgcagagtgg tttatcgggc tgttccaaaa gggcggagag gtgtttaccg ggatggtgac
     7861 cggcattctt ccgctgttga ttagcctgct ggttatcatg aacgcactga ttaattttat
     7921 cggtcagcat cgtattgaac gttttgctca acgttgcgcc ggtaaccctg tttcccgtta
     7981 cctgctgtta ccgtgcattg gcacgtttgt cttttgcaat ccgatgacct taagcctggg
     8041 tcgctttatg ccggaaaagt acaaacccag ctactacgcg gcggcctctt atagctgcca
     8101 ctcaatgaat ggcctctttc cccatatcaa ccctggcgaa ctgtttgttt atcttggcat
     8161 tgccagcggt ctgacaacgc tgaacctgcc acttggccca ctggcggtga gctatctgct
     8221 ggttggtctg gtcaccaact tcttccgcgg ctgggtgacc gatctgacca ccgctatttt
     8281 tgagaaaaag atgggcattc aacttgaaca aaaagttcat ctggcaggag caacatcatg
     8341 acgcgtattc ggatcgaaaa aggaacgggt ggctggggcg gcccgcttga gctggaagcc
     8401 acgccgggca aaaaaatcgt ctatatcacc gccggtaccc ggcctgcgat tgtcgacaaa
     8461 ctggcacagc ttactggctg gcaggctatt gacggattta aagaaggtga acccgcggag
     8521 gcagaaattg gcattgcggt aatcgactgt ggcggcacat tacgctgcgg catctatccg
     8581 aaacgacgta ttcccaccat taatatccac tcgacgggca agtccggtcc actggcgcag
     8641 tacattgtgg aagatattta tgtctctggc gtaaaagaag aaaacatcac tgtagtgggt
     8701 gatgcgacac cacaaccctc ttccgtgggc cgtgactatg acaccagcaa gaaaatcacc
     8761 gaacaaagcg atggtttact ggcgaaggtg ggaatgggta tgggttctgc cgtagccgtg
     8821 ttgtttcaat ctggtcgtga caccatcgac actgtattaa aaaccattct gccgtttatg
     8881 gcattcgttt cggcgctcat tggcatcatt atggcttctg gccttggtga ctggattgcc
     8941 cacggtcttg ctccgctggc gagccatcca ctgggtctgg tcatgctggc gctcatctgc
     9001 tccttcccac tgctttcacc tttcctcggc ccaggcgcag ttatcgcaca ggttatcggc
     9061 gtattgattg gcgtgcagat tggtctcggc aatattccgc cgcatctggc tttacctgcg
     9121 ctgtttgcca tcaacgcgca ggcggcctgc gacttcatcc cggtcggttt gtcgctggcg
     9181 gaagcccgtc aggacacggt tcgcgtcggt gtcccttctg ttctggtgag ccgcttttta
     9241 accggcgcac cgactgtact gatcgcctgg tttgtctccg gttttatcta tcaatagagg
     9301 ctgaaacatg accgttattt atcagaccac catcacccgt atcggcgcga gtgccactga
     9361 cgccctcagc gaccagatgc tcatcacctt tcgtgaaggc gcgcctgcgg atctcgaaga
     9421 gtattgcttc attcattgcc acggtgagtt aaaaggtgca ctccatcccg gtttgcaatt
     9481 ttcgctcggg cagcatcgct atccggtgac cgccgttggc agcgtggcgg aagacaacct
     9541 tcgcgaactg ggtcatgtca ccctgcgctt cgatggttta aacgaagcgg aatttccggg
     9601 cactgtccat gtggcaggcc ctgtccccga cgatatcgcg ccgggatcgg ttttgaagtt
     9661 tgaatctgtt aaggagtaaa aaatgaatca ggttgccgtt gtcatcggtg gtgggcaaac
     9721 cttaggcgcg ttcctgtgcc acggtctggc tgccgagggg tatcgcgtcg cggttgtcga
     9781 tattcagagc gacaaagccg caaatgtggc acaagaaatt aacgccgaat atggtgaagg
     9841 tatggcgtac ggttttggtg ctgacgccac cagtgagcaa agcgttctgg cgctctctcg
     9901 tggggtagat gaaatctttg gtcgcgtgga tttgctggtc tacagcgccg gaattgcaaa
     9961 agcagccttt atcagcgact tccagctcgg tgattttgac cgttcgctac aggtgaatct
    10021 ggtgggttat ttcctgtgtg cgcgtgaatt ttcgcgtttg atgatccgcg atgggattca
    10081 gggacgcatt attcagatca actcgaaatc cggcaaagtg ggcagcaaac acaactctgg
    10141 ctacagcgca gcgaaatttg gtggcgtagg gctgactcaa tcactggcgc tggatctggc
    10201 ggagtacggc attacggtgc attcactgat gctcggtaac ctgctgaaat cgccgatgtt
    10261 ccagtcgctg ttgccacaat acgcgaccaa gctgggtatc aaaccggatc aagtcgagca
    10321 gtattacatc gataaagtac cgctcaaacg cggctgcgat tatcaggatg tgctgaatat
    10381 gctgctgttc tacgccagtc ctaaggcatc gtactgcact ggtcagtcga tcaatgtcac
    10441 cggcggtcag gtgatgttct gatcaacagc ggagatccgt taaggatctc cgtgggacta
    10501 atagaatgcc tgatgcgcta cgctcatcag gcatacagga cttccgccac tacattaagg
    10561 aaaagttatg gtatccgcac tcatcactgt cgccgttatc gcctggtgtg cgcaactggc
    10621 cttaggcggc tggcaaattt ctcgttttaa ccgtgccttc gacacactat gccagcaagg
    10681 gcgggttggc gtgggccgtt ccagcgggcg ctttaaaccg cgggtcgtgg tcgccatcgc
    10741 cctggacgat cagcagcgca tcgtcgacac cttgtttatg aaaggactga ccgtcttcgc
    10801 ccgaccgcaa aaaattcccg caattaccgg tatgcatgcg ggtgatttac agcccgatgt
    10861 gatctttccc catgatccac tatcacagaa tgctctatca ttggcgctta aactgaaacg
    10921 tggataattt cgttgtgaat gttacttgct tgcgaagtta tcattttgaa acctaaatca
    10981 ggtaatcacg cccatgaaac ctcgtcagcg tcaggccgcc attctggagt atctgcaaaa
    11041 gcagggtaaa tgctcggttg aagaattggc gcaatacttt gacaccacag gcacaaccat
    11101 tcgcaaagat ctggtcattc tggaacatgc cggaaccgtc attcgtactt atggcggagt
    11161 ggtgttgaat aaagaggaat ccgatccgcc tatcgatcat aaaacactca tcaacaccca
    11221 caagaaagag ctgattgcag aagctgccgt tagttttatc catgatggcg attcgatcat
    11281 tcttgatgct ggcagtaccg ttttgcagat ggtccccctg ctctcgcgct ttaataacat
    11341 cacagtgatg accaacagcc tgcatatcgt caatgcgcta tccgaactgg ataacgaaca
    11401 aactatcctg atgccaggcg gaacatttcg taaaaaatcg gcctcatttc acgggcagct
    11461 ggcagagaat gccttcgagc atttcacctt cgataaattg tttatgggca ccgacggcat
    11521 cgatctcaat gcgggcgtaa ccaccttcaa cgaggtttat accgtcagta aggcaatgtg
    11581 caatgccgcg cgcgaagtga ttttgatggc ggactcatca aagtttggcc gtaaaagccc
    11641 caatgtagtt tgcagtcttg aaagcgtcga taagctaatt accgacgcag gtatcgatcc
    11701 ggcgtttcgt caggcgctgg aagagaaagg gatcgatgtg atcataaccg gagagagcaa
    11761 tgagtgaagc actactgaac gcgggacgtc agacgttaat gctggagttg caggaagcaa
    11821 gccgcttacc ggaacgtctg ggcgatgatt ttgttcgcgc cgccaatatc atcctgcact
    11881 gcgaaggcaa agtggtggtt tcgggaattg gcaaatcggg ccacattggt aagaaaatcg
    11941 ccgcaacgct tgccagtacc ggcacaccgg ctttttttgt ccatccggca gaagcgctgc
    12001 acggcgatct ggggatgatc gaaagccgcg atgtgatgct gtttatctct tactccggtg
    12061 gcgcgaagga actggatctg attattccgc gtctggaaga taaatctatc gcgctgctgg
    12121 cgatgaccgg caaaccgacg tcaccgctgg gcctggcggc gaaagcggtg ctggatatct
    12181 ccgtagaacg cgaagcctgc ccgatgcacc ttgcgccgac ctccagcacc gtcaataccc
    12241 tgatgatggg cgacgcgctg gcgatggcgg tcatgcaggc gcgcggattt aatgaagaag
    12301 attttgcccg ctcccaccca gccggggcac tgggcgctcg cttgctgaat aaagtgcatc
    12361 atctgatgcg tcgtgatgac gctatcccac aggtagcgtt aaccgccagc gtgatggatg
    12421 cgatgctgga actcagccgc accggtctgg ggctggtggc ggtatgtgac gctcaacaac
    12481 aggtacaagg cgtctttacc gacggcgatt tacgtcgctg gctggttggc ggcggcgcac
    12541 tcaccacgcc agttaatgaa gcgatgacga caggcggtac cacattacag gcacaaagcc
    12601 gcgccatcga cgccaaagag atcctgatga agcgcaaaat cactgccgca ccggtggtag
    12661 atgaaaacgg caaactcgtc ggcgcaatta acctgcagga tttctatcag gccgggatta
    12721 tttaatcctt caatcccaga cgtttcgcca gccgatgcag gttggcgacg tcggtttcca
    12781 gcattcgcgc gcaggctgcc cagttgtgat gattttgtgc cagtgcctga cgaatagttt
    12841 cacgctggaa cgcttctgtc gcttcacgca ggttttgctt aacaacgggc accgccgccg
    12901 cttctggcgg cggtaacgtc acctcaggaa aagcaaaatg ttgcgcctca agaatcactt
    12961 catcgccgtt gcgggtggct ctcgccagca ctaccgcccg atgaatagca tgttccagtt
    13021 cgcgcacgtt ccccggaaaa cgatagtgtt gcagtaaatt tcgcgctccg gcacttaata
    13081 ccacgcggga gagccccagc cgcaaacgac actgctcgca gaaatacccc gccagcagaa
    13141 tgacatcatc gccccgctca cgcagcggcg gcaccgaaag tggaaacacg ctcaggcggt
    13201 ggaacaagtc agcgcgaaat cgccctgcca gcacctcttc gcgtaaatcg cggttagtcg
    13261 ccgccagcac gcgcacatcg acccgcaaac tacggtcatc gccaacgcgc tgaatatcgc
    13321 catactgcaa caccctcagc agcttggcct gcaatgccaa cgacaactcg ccgatctcat
    13381 cgagaaacag cgtgccgtta tccgccattt caaacttccc actgcggtta ctgatagcgc
    13441 cagtaaacgc tcctttcaca tgcccgaaca actcactttc cgccacactt tccggcagtg
    13501 cggcacagtt gagatagacc agcggattca ccgcccttgg cgaggcttca tgaatcgctt
    13561 tcgccaccag ctccttaccg gttcccgtct caccgctgat taagacgttg agatcggacg
    13621 ccgccacaat ctcaatctct tttttcaatt gcgtcatgcc tggcgataag ccaatcatct
    13681 gcgtctgttt caccgcttca aacggcgcgg catcgcccgg cagcatattc tggctttcca
    13741 gttgctcaat cagcaacgca ttgcttaacg ctcccgccgc cagcgcggca atcaggcgta
    13801 actcttcgtc gctgaaaaca tcgaactgat cgggctgcat cccgtcgagc gtcaatgcgc
    13861 cgatcaggtt ttgcccggca aacagcggca gaccaacgca ggcgtgaacc ttcagactct
    13921 cctgccctgg aatcaaaccg tcatagggat cgggcaattc gctgtctgcg ggaaagcgca
    13981 ccacatcacc cgcgcgggca atcgcttcca gccgtggatg accttccagc gcaaagcgtc
    14041 taccgagtac atccttcgcc agcccgtcga tggcaagcgg aataaactgc cgcgagtcgt
    14101 aacgtagcaa cgcagacgca tcgcactcca gcacctgacg cagcgtggtg atcaggcgct
    14161 gaaaacgatc ctgatgacca atcccacgct gcaattcgat ggcgatattc gccagcacat
    14221 caacggaaaa actcatcttt gcctcactgt caatttgact atagatattg tcatatcgac
    14281 catttgattg atagtcattt tgactactca ttaatgagca taattttatt tatagagtaa
    14341 aaacaatcag ataaaaaact ggcacgcaat ctgcaattag caagacatct ttttacaaca
    14401 cgctgaataa attgaggttg ctatgtctat tgtggtgaaa aataacattc attgggtggg
    14461 tcaacgtgac tgggaagtgc gtgattttca cggcacggaa tataaaacgc tgcgcggcag
    14521 cagctacaac agctacctca tccgcgaaga aaaaaacgtg ctgatcgaca ccgtcgacca
    14581 taaattcagc cgcgaatttg tgcagaacct gcgtaatgaa atcgatctgg cggatatcga
    14641 ttacatcgtg attaaccatg cagaagagga ccacgccggg gcgctgaccg aactgatggc
    14701 acaaattccc gatacgccga tctactgtac agccaacgct atcgactcga taaatggtca
    14761 tcaccatcat ccggagtgga attttaatgt ggtgaaaact ggcgacacgc tggatatcgg
    14821 caacggcaaa cagctcattt ttgtcgaaac accaatgctg cactggccgg acagcatgat
    14881 gacttacctg acaggcgacg cggtgctgtt cagtaacgat gctttcggtc agcactactg
    14941 cgacgagcat ctgttcaacg atgaagtgga tcagacggag cttttcgagc agtgccagcg
    15001 ttactacgcc aatatcctga cgccgttcag ccgcctggta acgccgaaaa ttaccgagat
    15061 cctgggcttt aacttaccag tcgatatgat agcgacctcc cacggcgtgg tctggcgcga
    15121 taacccgacg caaattgtcg agctgtacct gaagtgggcc gctgattatc aggaagacag
    15181 aatcaccatt ttctacgaca ccatgtcgaa taacacccgc atgatggctg acgctatcgc
    15241 ccaggggatt gcggaaaccg acccacgcgt ggcggtgaaa attttcaacg tcgcccgaag
    15301 cgataaaaac gaaatcctga ccaatgtctt ccgctcaaaa ggcgtgctgg tcggcacttc
    15361 gacgatgaat aacgtgatga tgccgaaaat cgccgggctg gtggaggaga tgaccggatt
    15421 acgcttccgt aacaaacgcg ccagtgcttt cggctctcac ggctggagcg gcggtgcggt
    15481 ggatcgtctt tccacgcgcc tgcaggatgc gggtttcgaa atgtcgctta gcctgaaagc
    15541 gaaatggcga ccagaccagg acgctctgga gttatgccgt gaacacggtc gcgaaatcgc
    15601 ccgtcagtgg gcgctcgcgc cgctgccgca gagcacggtg aatacggtag ttaaagaaga
    15661 aacctctgcc accacgacgg ctgacctcgg cccacggatg cagtgcagcg tctgccagtg
    15721 gatttacgat ccggcaaaag gcgagccaat gcaggacgtt gcgccaggaa cgccgtggag
    15781 tgaagtcccg gataacttcc tctgcccgga atgctccctc ggcaaagacg tctttgacga
    15841 actggcatcg gaggcaaaat gagtaacggc attgtgatca ttggttcggg cttcgccgcc
    15901 cgccaactgg tgaaaaatat tcgcaaacag gacgccagta ttccattaac cctgattgcc
    15961 gccgacagca tggatgagta caacaaacct gacctcagcc atgttatcag tcaggggcaa
    16021 cgtgccgacg accttacccg ccagacggcg ggtgaatttg ccgagcagtt taatctgcta
    16081 ctgtttccgc aaacttgggt gacggatatc gatgccgaag cccgtgtggt gaaaagccag
    16141 aataatcagt ggcaatacga caagctggtg ctggcaaccg gtgccagcgc ctttgtcccg
    16201 ccagtgcctg ggcgtgagtt aatgctgacg ttaaatagtc agcaagagta tcgcgcctgt
    16261 gaaacgcaac tgcgggatgc ccgacgcgtg ttgattgttg gcggtggttt gattggtagc
    16321 gaactggcga tggatttttg tcgtgcaggc aaaatggtta cgctaatcga caacgctgcc
    16381 agtattctgg cgtcgttaat gccaccggaa gtaagcagtc gcttgcagca tcggttgacg
    16441 gagatgggcg ttcatctgct gttgaaatct cagttgcagg ggctggaaaa aacggattct
    16501 ggcattctgg caacgctgga ccaccagcgc agcatcgaag tggatgcggt aattgccgcc
    16561 accggactgc gcccggaagc cgccctggca cgacgcgccg ggctgacgat taatcgcggc
    16621 gtttgcgtcg atagttatct gcaaaccagt aatgccgata tttacgcgct gggcgattgc
    16681 gcggaaatta acggtcaggt attgccgttc ctccagccga ttcaacttag cgcgatggtg
    16741 ctggcaaaaa atcttctcgg caataacacg ccgctgaaac taccggcgat gctggtgaaa
    16801 atcaaaacgc cggaattacc gctgcatctg gcaggcgaaa cccagcgtca ggatttacgc
    16861 tggcagatta ataccgaaag ccagggaatg gtggcgcgcg gtgttgacga tgcagaccaa
    16921 cttcgcgcct ttgtggtcag tgaggatcgg atgaaagagg catttggatt gttgaaaaca
    16981 ttgccggtgt aggcgggcta ctgtgcccaa aatgtcggat gcgacgctga cgcgtcttat
    17041 ccgacctacg gggacgcatg tgttggtcac ataatgcgtt catgtcgcat ccgcctactg
    17101 tacccaaaat gtcggatgcg atgctggcgc gtcttacccg acctacgggg aggcatgtgt
    17161 tggtcacata atgcgttcat atcgcatcca gctactgtgc ccaaaatgtc ggatgcgatg
    17221 ctggcgcatc ttatccgacc tacggggagc gcatgtgttg gtcacataat gcgttcatgt
    17281 cgcatccagc tactgtaccc aaaatgtcgg atgcgatgct ggcgcatctt atccgaccta
    17341 cggggacgca cgtgtaggcc ggataaggcg tttacgccgc atccggcaat ggtgtccaaa
    17401 tgcaacacgt tttatccgtt ctggacttca cccgctaacc aacgcgccgc agcaataacc
    17461 ccctgcccca gagacaaacc gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg
    17521 aaatcagcga gataatgcgc cagacgtgca cgcagcaaac ggttatgaat aaccccgccg
    17581 ctaaatacca gcgtagtgat accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa
    17641 ccctgcgcca gcgcatcatg aaacgcccac gcgcgttgat taaccggtgc ctgccagttc
    17701 agccactgct gccagaaagt ggcgagatcc agttgattgt ccaccagcgg cattgtcacc
    17761 ggatgcgtca ctccgtggca tgaggctgcg agcgcctcca gagcacaagc cgcttcacct
    17821 tcataactta acgtggctgg cgcacagccc agtgccgccg ccactgcatc gaacacccgc
    17881 ccacacgatg acgccagcgg cgcgttaatt ccacgctcaa tggcccgcgc cagaacgctc
    17941 cagttttgct gttgtacact tgccgtttca gggtaattct gccactccgg cacaaagcgc
    18001 aggcactgcg ccagcaggtt tcgccacggc tgcttcgccg ccaaatcgcc acccggaagc
    18061 gccactgcag gcaagccgcc caggtgctgg cattcgcgat agttcacccg caggcactcg
    18121 ccgccccaca aagcgccgtt ctcccccata ccgataccgt cgagcgtcaa agcaatgaca
    18181 tcaccgccat ccagtggcca gtgatgttct gccagacacg ccgctgcgtg ggcatgatga
    18241 tgcagtaccg tttgcgtcgg cagattcatc tcgctcgccc actggctgga gacatagccc
    18301 ggatgtgcgt catgcacaac gtattgcggg gtgaaatcgt agatgttttg catcaggcgt
    18361 aacgcttcgc gccactgcat ctggatgcca tcgtcactta aatcgcccag atgctgactc
    18421 aacaccgctt gttcaccgcg caccaggcag aaggtgtttt tcagatccgc gccgagacac
    18481 agcacaggcg gaacattttt aaagcccgga ggcaaagcca gcgcatccgg cacatacccc
    18541 cgcgaacggc gcagcatttc gccgctttcg cgcaccaccg aatcatccat ccgctgcacg
    18601 atgtcgcggt tatgtatcaa gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt
    18661 tcgttgctga tagccggtgg tttaccgctc aggttgccag aggtcatcac cagcgggcat
    18721 tgcagttcct gtaacagcaa atgctggagc gggttcgcag gcaacattac cccgacttcg
    18781 ttaaggtcag gggcgatatc atcacaaagc tcaggtacgt attttttatc caccagcaca
    18841 atcggcgcgg cgggcgtggt aagcaactgg cgcgcagcgt ctggtaaacc gtcagccact
    18901 ggcaacatga ccgccagcgg tttcgccggg cgatgtttgc gcgcccgcag tgtcgccacc
    18961 gcgttactgt tacgtgcatc gcaggcaaga tgaaatccgc caatcccttt gatggcgaca
    19021 atgttgccca tttttaactg tgcgatagcc gcctgtaatg ccgcctcttg ttccgcatgt
    19081 tcaccatgac ttacccattc aagatgcggg ccacactccg ggcaggccac cggctgggcg
    19141 tggaagcgac gatcgagcgg gtcgcggtac tctttgtcac aagccggaca caacggaaac
    19201 gccgccatta cggtaaacgg gcggtcgtaa ggcatggcgc gaataatggt gaaacgcggg
    19261 ccacagtgag tacagttgat aaacggataa cgataacgcc gctcgcctgg ggtattcatt
    19321 tcggcaaggc aagcagggca agtagcggca tccgggacaa tttgcgtatt catggcacct
    19381 cccgcgctct ggcggatggt gaactcagtg ggcagttgtg accagatgta cggctcacgc
    19441 tcgacgctat caatacgcgc cagcggcggg cagtgctgat gcaattgaac aagaaacgtt
    19501 tccgggtctt ccagcagccg gacttctacg ccatcgccgt cattacagac atcgccgtga
    19561 agatttaatt gctgtgccag ttgccagaca aacggacgaa aaccgacgcc ctgcactttg
    19621 ccacgaatac gcagttggac accgcaagat gtgttttttg acattgagtt attcccgcca
    19681 tcatgaattg cgtaacccgc ccagccggac acgacagcgt cgcatccggc agtcacaggt
    19741 cggcgatacc gcccgctccg tattctacga atatttccgg gaattccttt gatgccagaa
    19801 cagttctgta agatttttag aacatcagcg ccgtacggcg gcgtttttct gcgctcagtt
    19861 gttcaagttt attacgatcg acacaaatca gcgcatgagt cgggcaagcc gccatacacg
    19921 ccggaccgtc ttcacgatgg ttgcacaggt cgcatttatt ggcttcggct ttgtcagccc
    19981 gtacattcag ccctgcgccg ctgttgcgaa tcaccggacg taccaccact tccatcgcgc
    20041 catacgggca agccacaacg caggttttgc aaccaatgca acgttcctgc atcacatgaa
    20101 caaacccttt atcacggctg atagcaccat tcgggcagac gttagcgcac ggtgcatctt
    20161 cacactgacg gcaaactgtc gccgtggaaa tgttcacacc tttaatgaca tggatacgcg
    20221 gtaaaaatgt ttccggggtc agcgatgcac agtcctgatt ttcctgatga gaaaccacgc
    20281 acgccacttc acaggtacgg caaccaatac atttactcgc atcagcaatg atgaaacggt
    20341 tcatcaaatt ctccagcaat gacagttaat gcgccgatac attcacaaat catgccagtt
    20401 tttaattcac tgttatttaa ggaaattaaa ttctgtgatg caggaaaaac gatgtcatcg
    20461 acactagtga cgatgacatg tgatgacagt gtttatcgcg gtaaagcttt gagtgagtcg
    20521 cggcagatca attttccgct gaaggttttc ggcggtgtgt aagcgccccc atcgagcata
    20581 aaaatcagcc gtccaataac ttcctgaatc atctcagtca ccggaatttt tacgctggag
    20641 agcgccggaa cggtgtaggg ggcaattgcg atatcatcga atccgataac tgacacctgc
    20701 tctggcaccg ctacgccgcg ctcgtgtaac gctttcatcg cacctatcgc catatcgtcg
    20761 ttactggcaa ctaacgcgct aaatttaacc ccacgttcga gcaacatttc taccccttcg
    20821 gccccgctgg cgggcgtcca tttaccgttc gcgataagtt tttcattgag cgcaatacca
    20881 tgctgcgcca gcgcgtcttt atacccggca agacgttcaa cgctggtggg ggaatccatc
    20941 gagccggtaa ggaaagcaat ctcctgatgt ccggcgttta tcaactctgc cacggcgtta
    21001 aagctggtct gtttatgatc gcaccagacg ctatggctgc tatttttgcg caggcggcga
    21061 ttaagcacca ttatcggctg actgtgcgcg tcaatgatgt catcaatttc atccacgctt
    21121 aaaaagcgcg gataaatcat gatcgcgtcg cagcgcagat ccagcagata ctgaatcgcc
    21181 tggcgctctt cttcggcgct gtgtttacca tctgccaata gcaactgccg ccctttctct
    21241 tccgccattc gcgcggcatg aaacagtaat tcactaaaat aaatgccgtg gtaaagcgtg
    21301 ttggtcacta ccagccccag cgtctgagta ctcttcgccg acagattgcg cgccagcaag
    21361 tttggacggt aaccgctctc ttctaccgcc tgaaacacgc gatctttagt ctcctggctg
    21421 acgtagccat tacctgaaag cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc
    21481 gccacttcca gcatcgtcgt catcattttc atccctttac acgcaatcaa cgcagtgtac
    21541 tgcaccgttt gccgattgtc cttgcgcaat cggcgggaaa aatattcagg tgaccggttt
    21601 cacaaatata aaaaatgaac aattaactct cttgcttatt tagtggcaac tattcatgat
    21661 tttgtgaaac cggtttctta attccgtttc agcatcggca tttttccgtc acgtcgactg
    21721 ataacaacta catctaccct actgataaca ggataaaatc cgatggccaa aaattatgcg
    21781 gcgctggcac gctcggtgat agcggcactg ggcggcgttg ataacatctc ggcagtcacg
    21841 cactgtatga cgcgtttgcg ctttgttatc aaagatgatg cgcttatcga cagcccgatg
    21901 ttaaaaacca tccccggcgt gctcggcgtg gtacgtagtg acaatcagtg tcaggtgatt
    21961 atcggcaata ccgtttcaca agcctttcag gaagtcgtca gcctgctgcc gggagatatg
    22021 cagcccgcac agcccgtggg taaacccaaa ctcacactgc gtcgcattgg tgcggggatc
    22081 ctcgatgcgc tgatcggcac catgtcaccg ctgatcccgg cgattatcgg cggatcgatg
    22141 gtcaaactgc tggcgatgat cctcgagatg agcggcgtgc tgacaaaagg atcgccgacc
    22201 ttaaccattc tgaatgtgat tggtgacggt gctttcttct tcctgccgct aatggtcgcg
    22261 gcatctgccg ccatcaaatt taaaaccaat atgtcgctgg cgattgccat cgcgggtgtg
    22321 ctggtacatc cgagttttat tgaattgatg gcgaaagcgg cccagggtga acatgtcgaa
    22381 tttgccctga ttccggtcac cgcggtgaaa tacacctaca cggtgatccc ggcgctggtg
    22441 atgacctggt gcctgtcata tatcgaacgc tgggtggatc gcattacacc ggcggtgacc
    22501 aaaaacttcc tcaaaccaat gctgattgtg ttgattgccg caccgctggc aatcctgctg
    22561 attggcccga ttggtatctg gatcggtagc gccatttcgg cgctggttta caccattcat
    22621 ggttatctgg gctggctttc agtcgccatt atgggcgcgc tgtggcctct gctggtaatg
    22681 accgggatgc accgcgtctt tacgccaacc atcattcaga ccattgccga aaccggcaaa
    22741 gaagggatgg tcatgccgtc agagatcggc gctaacctgt cgctgggcgg ttcatcactg
    22801 gcggtggcgt ggaaaacgaa aaacccggaa ctgcgccaga cggcgctggc tgcggcggca
    22861 tcagccatta tggcggggat ttccgaaccg gcgttatacg gcgtggcgat ccgcctgaaa
    22921 cgtccgctta tcgccagtct tatcagcggt tttatttgcg gcgcggttgc cggtatggcg
    22981 gggcttgcca gccactcaat ggcagcgccg gggctattta ccagcgtgca gttcttcgat
    23041 ccggcgaatc caatgagcat cgtctgggtg ttcgcggtca tggcgctggc ggtcgtgctg
    23101 tcgtttatcc tcacactgtt gctcggcttt gaggatattc ctgttgagga agcggctgcc
    23161 gagacgcgaa agcatcagag cgcacaaccg accgtcgcca aagaagtaag tcttaattga
    23221 ggatgaaaat gtcagtattt ccagaaggtt ttttatgggg cggcgcgctt gccgctaacc
    23281 agtctgaagg tgcgttccgt gaaggtggca aagggctgac cacggtcgat atgatcccac
    23341 acggcgagca tcgaatggcg gtgaaactgg ggctggaaaa acgttttcag ttgcgcgatg
    23401 acgagtttta tcccagccat gaggcgacgg atttttatca tcgttataaa gaagatatcg
    23461 ccctgatggc agagatggga tttaaggttt tccgcacctc aattgcctgg agccgcctct
    23521 tcccgcaggg cgatgaactg acgcccaatc cgcagggcat tgctttttat cgttctgtct
    23581 ttgaagagtg taaaaagtac ggtatcgaac cgctggtcac gttgtgccac ttcgatgtgc
    23641 cgatgtatct ggtcaccgaa tatggctcct ggcgtaaccg caagctggtg gagtttttca
    23701 gccgctacgc ccggacctgc tttgaagcat ttgatagcct ggtgaaatac tggctaacct
    23761 tcaatgaaat caacattatg ttgcatagcc cgttttccgg cgcgggtctg gtgtttgaag
    23821 aaggtgaaaa tcaggatcag gtgaaatatc aggctgcgca tcaccaactg attgccagtg
    23881 cattagccac caaaatcgcc catgaggtta acccgcaaaa tcaggtgggc tgtatgctgg
    23941 cgggcggcaa cttctaccca tacagctgca agccagaaga tgtctgggcg gcgctggaga
    24001 aagatcggga aaacctgttt tttatcgatg tgcaggcgcg gggcgcgtat ccggcttact
    24061 ctgcccgcgt atttcgcgaa aaaggggtaa ccatcaacaa agcaccgggc gatgatgaga
    24121 tcctgaaaaa caccgtcgat tttgtctcct tcagctatta cgcctcgcgc tgcgcctcgg
    24181 cagagatgaa cgccaataac agcagtgcgg cgaatgtagt gaaatcgctg cgtaacccgt
    24241 atctacaggt aagcgactgg ggctggggta ttgatccact cggtctgcgt atcaccatga
    24301 atatgatgta cgaccgttat cagaagccgc tgtttctggt ggaaaacggc ctgggcgcaa
    24361 aagatgaatt tgctgccaat ggcgagatta acgatgacta tcgcatcagc tatttacgcg
    24421 aacatatccg cgcaatgggc gaagcgattg cagacggcat tccgctgatg ggctacacca
    24481 catggggctg tattgattta gtttccgcct ctacgggtga aatgagcaaa cgctacggtt
    24541 ttgtctacgt agaccgtgac gacgcaggaa acggtacgct gacgcgcacg cgtaagaaat
    24601 cgttctggtg gtataaaaaa gtgattgcca gtaatggtga agatttagag taagtaacag
    24661 tgccggatgc ggcgtgaacg ccttatccgg cctacatgtc gcacgaacca ggaaagcccg
    24721 ataagcgcag cgcatcgggc aatttagcat ttggcatcag agctggttca ccccatcaag
    24781 aacatccctg tcctgattac ttattgaaaa actactcttc ttccaccgct aactgcgcga
    24841 agccgccgtt cccttcccag ccttccagtc gttgataaac ggtttctacc gcatctttaa
    24901 tcggctgggt catcgggtag taaaagccga cgatatccgg ctggatgccg aggaaaatca
    24961 cttcgccaat atcttctttc aactgatcga taaggtagtt caacggcatg ttatgggtag
    25021 tcatcataaa catctcggcg atatcatccg ggtcgatgat gcggatttcg ccggggttta
    25081 gccccatatc agtggcgtcg acaatcagca gtcgtgtcgg gcgcagttca cggatagcga
    25141 cgatgtcgtt ttccggtgcg ctaccgccgt caatcaccac ccagttacct ttcggcgcgg
    25201 cggcgcactt ttccgccagc agcggacctg cgccatcatc gcccatcatg ctattgccaa
    25261 cacagagtaa aacgtcagtc acgcagtctc ctcaccatca aatagatggc gttttcctga
    25321 tgaatatcat gcagcatact taacagcatt gtactccatt cctgctgctg cgaagtttgc
    25381 gctgcccggg ctttatcgaa tgcattggcc agcatcggca catggttaat gtcgatgacg
    25441 atctcaccgt acttcggcac accttccatt ttgcggcggg cttcgctgcc cgcctccaga
    25501 gtggcaatcc atgccagata ttcatcccac gggcaggtga gcgccgcttc caggcaatcg
    25561 ataaccccaa ggtggtgacc aatcgccagg ctgtaataga ctacctgctg cgcctcggcg
    25621 ggcgtggcat cgttctcatc aataaattta cggctcagtt gactgaacac caccttttca
    25681 ctcatcggat acgcgcctct tcaacaacat gattcagatg gctgacaatc tcgttcagac
    25741 gcggatcgtt ttccgcttcc agccagcgtg caacctgctc ttcgccctgc cctaattgcg
    25801 tcaggaagtc atcggcaatc tggcgaccgt aacgataacc cgccaggcgg cgtgcttcgc
    25861 gatccacttt cacgcgcagt ggctgcacca tatcgccatg cagaatctca gccggttgtt
    25921 catcctgctc actcggccca cgggcgtgaa ttttctgctc cagcaggccg agcgccattg
    25981 caaagccgta cagcgtggcg gcaggcgttg gcgggcagcc agggatataa acatccaccg
    26041 ggacgatttt atccgtaccg ccccacacgc agtagagatc gtggaagatc ccgccgctgt
    26101 taccgcaggc accgtaggag atacaaattt tcgggtccgg cgcggactgc cacgcacgca
    26161 gcgcagggga tcgcattgca cgggtgaccg cgccggtaaa cagtaaaata tccgcatgac
    26221 gcggtgaagg aacgacttta atgccgaagc gttctgcatc aaacagcggc gaaagcgtgg
    26281 cgaaaatttc gatttcgcag ccgttgcagc cgccgcagtc cacgcggtaa acgtaggcag
    26341 aacgtttgat ttttttcagt aacgacgcct tcatgctggc gatggattca tccaccgtca
    26401 tcgggaccgg aatgccgttg gcgtcacggg ggcctaataa attgctcatc agatggcctc
    26461 tttcatatgg cgagtcagtt caatacggtc ggacggcacc aggcatttct ggcgcttaca
    26521 ttccgggcaa gtctcaaagc tttcgcggtg gttttccgcg cggctgtcac cattgtgctt
    26581 aagcagagca atggcgtagt cgatctcttt ctggacggcg aaaggacgat tgcagacgcg
    26641 acagttgcac agcgcgaagc gggactgttg caggaagtct tctttcttcc acaccgccag
    26701 ttcgtactct tgcgacagtt tgattgccgc cgtcgggcaa acttcttcgc agcgtccaca
    26761 gaagatgcag cgcccgagat taaactccca ggccagctct cctgtggcga ggtcagtttc
    26821 aaccgttaag gcgtttgacg ggcaggcatt gacgcaggcc gcgcagccga tgcactgctg
    26881 cgggttctgc tctggcttac cacggaagtt tttatcaacc gcaatcggct ccagcggata
    26941 agacgaggtc gccgtgccgg ttttgatgac ttttttgata aaggtaaaca tggcgattcc
    27001 ttatttcagc ggcgagtttt tacgctcaat gctgtaacgc tcgagttctt tgtacggcac
    27061 cactttgctc ttcttcttac gcacatcgac cacggtcatg cggtcggtac aggagtagca
    27121 agggtcgagg ctaccgataa tcagcggcgc atcggaaacg gtattgccac gcagcatgta
    27181 gcgcagggtc ggccagttcg cgtaagtcgc ggcacggcag cgccagcggt acagcttctg
    27241 gttgtcgccg gtcatgctcc agtggatatc atcgccgcgc ggcgcttcgg caaagcccag
    27301 cgcaaagcgg tgcggaatgt aggtaaagcc ttccaccatc agtgggccac ccggcaggtt
    27361 atccagaccg tagtcgatca tgttcagcgc ggtatagact tcgttgatac gcactttcag
    27421 acgggaaata acgtcgcagc cctgctcgct gtggacttcc attggcagca ggccataacc
    27481 aacaaacggg tgatcggcgc gagtatcgcg agcgtgaccg ctggcacgga ccatcgggcc
    27541 gacgttactg aagtcgcgag cgatttccgg gtccagacga ccaatgccga cagtgcgttg
    27601 ttccatgttc ggtgtgctca acagcacatc caccagctcc tgcacttcac gacgcatctg
    27661 ttgcgccagc tggcgggtct ggatcatgtc atctttcagc agatcgcgac gaatcccgcc
    27721 gatcaggttc aggccgtagg ttttacgcgc accggtaagg atctctgcca ttttcatgga
    27781 ggtttcacgc acgcggaaga actgcataaa gccggagtca aagccggtaa agtggcaggc
    27841 aagaccgagg ttgagcagat gcgagtgcag gcgttccacc tccagcagaa tggcgcggat
    27901 catctgcgca cgttctggca cctgaatacc catcgcgttt tccaccgacg tggtgtaggc
    27961 ggtgctgtga gcaaagccgc agatcccgca cacacggtca gagaggaagg tcacttcgtt
    28021 gtaacccata cgggtttccg ccagtttttc catgccgcga tgaacgtaga acagacggta
    28081 gtcggcgtcg ataatgtttt cgccatcgac gaacagacgg aaatgacccg gttcgtcaga
    28141 agtgacgtgc agcggaccaa tcggcacgac gttgtttttc ttgtcgccca gttcgttgat
    28201 gaactcgtag gtttcagcat cggtggtcgg tgccggacgt tgacgataat ccatgctgtc
    28261 tttacgcagc ggataaagtt catccggcca gtcatccggc agcaccagac ggcgttcatc
    28321 cggcagccct accggaatca aaccgtacat atcgcgcact tcgcgctcgc cccacaccgc
    28381 cgccggaacg cgcggcgtca cggacggata ctccggtttg ttggcatcaa cttcgacgcg
    28441 aaccgttacc caacacttgg tgcccttctc catcgacagc acgtaataaa cggcgtaatg
    28501 accattcagt ttgcgttcgt cgttaccaaa cagcaccgac agccagccac tctgtttgta
    28561 gtaaagaaac tccaccactt ccggcaggta gttcaccttt acggtgacag tcagctgatc
    28621 tttggtctgc caggcgtggt ccagcacgac gcccggaaat gcctcattca gcgcggcgag
    28681 ataatgttga cctaattttt cttcagacat gctcaaactc tctttaatca cgccgccagc
    28741 aaggagacga acgctaaaaa tgcaaagcca aacccagccc aggtaatgcg cggagtaata
    28801 tcaagacgca gacgggccat gctgttttcg aacagcgcga taaccaggac gccgaccacc
    28861 agtttgatga tggcaatcac cagcgccagc agcagcccac cgacggtgaa ggtttccatt
    28921 tgtccccacg gaataaacac cccgacgaac atctgcagca ccaccaactg tttcaggctg
    28981 ataccccatt tcatgacgcc aaagccgctg ccgctgtact cagagagcgg accttcctgc
    29041 aactcctgct cggcttccgc caggtcgaac ggcagtttgc ccatttcgat aaaagtggcg
    29101 aacgcacagg cacaaagcgc cagcaccagc gggatgctct ggctcagcgg ccagtgataa
    29161 acggtgtcgg tgatgttgct gatgttggtg gaaccggcaa cctgtgcggc aacccacaga
    29221 ccaagcagca gcatcggttc gaccagcacg ccaagcatcg cttcacggct cgcgccgata
    29281 gcagtaaacg ggctaccggt atccagaccg gaaatggcaa agaagaaacg cgcgatggca
    29341 aagagataca gtaaggtgat caaatcgccc agttgcggca gcggagaacc aacggtcacc
    29401 accggcagcg cagtagcgat agtcagcatg acgcccacca tcacatacgg cgtcaggcgg
    29461 aacacccagc cggaggcatc cgggccaacg ctctgacgcc ccagcagttt gataatgtcg
    29521 cgatactcct gcaacacgcc cggcccgcga cggttatgca agcgggcgcg cgccacgcgg
    29581 gttataccgg agagcagcgg cgcaacggca aataacacca gcgcctgaat taacggatat
    29641 aaaacactca ttctcaggct cctcgtgaaa caataatcac caccagtacc gccagttcaa
    29701 ccagcgccat ccggcggaac agcaacgcac tcccctcgca ctgccagccc ggcaccagcg
    29761 acaccggatt cagccatttg cgcagtttca gcaccggcgc aaacgcctgt ttcaccggca
    29821 tggcaaaacc gtgagcggta atcaccattg atttttcgtg gtcgtagccg cacacccagg
    29881 ccgcgccgcg ggaacgcgat ggcaaacgat cgcctttgca aatcgccata atgatgaatg
    29941 gcagcagcgg gcaggcaatc agcagcaacg tgatcatcgg ttgagaaacg gtggtgttag
    30001 caggctccag cggcagaggt acagcagcag agagcatcgg caatagccac ggcgcagcaa
    30061 caccaccaat tacgcagcaa atcgccagtg ccactacgct tacgctcatc aggagcggcg
    30121 cacaggtggc gttttcggct tctttggtac gcggcgcacc gaggaacgtg acgccataga
    30181 ctttcgccat acacatcacc gccagcgcac cggtaattgc cagacccaca gcaagcagtg
    30241 gccccagcag acgggcaaca aacgcgccac tattgctcag tttgaaaaag gattgataga
    30301 taacccattc cccggcaaac ccgttcagcg gcggcagcgc agccattgcc atcaacccga
    30361 ctaacatggc gatggagata accggcattt tcttgccaat accaccgagt ttttcgatat
    30421 cgcgatgacc ggtacggaac cagacgctcc ccgccccgag gaacagtacg cttttgaaca
    30481 ggctatggtt aagcagatgg tacagaccac cgaccaggcc aagagcaatc agcgccggtt
    30541 gttcgagcgc gatacccgtt acgccagcgc ccagccccag caggataatg ccgatatttt
    30601 ccagggtgtg gtaagccagc agacgctgga tgttatgctc catcagcgca tacagaccac
    30661 cgacgaacgc ggtgatcata ccaagcacca gcagcacgat cccccaccac agcggtgcat
    30721 taccgcccag cagtgacagg gttaaaatgc ccagcaggcc aattttcatg actaccgtag
    30781 aaaacaacgc ggcagctggc gcagaggcgt tcgcatgtgc ctgcggcacc cagccgtgca
    30841 gcggaataat cccggccagc aggccaaagc caatcacact gagcagccag atatcggaac
    30901 cgagcggcag ctgttgcata cgcatatcca gcaggcgcag atccagcgtg ccgtaacgct
    30961 gccacagcag ccagcaagca atcgccagca gcagagtgcc aagacgcccc agcgcaaacc
    31021 acagtttgcc ctctttgctg ttgctggtga ggaacaccgc acacaaggcc atgacttcgg
    31081 ccattaccac gaacatgccg aggttgctgg caatgacggc gcagacggcg gcagccatca
    31141 acatattgat ctgcaagccg ttgcacttca cctgcgcgtg gcgatgccag tcaatgttgt
    31201 agaggctgac aaacagaccg cacagaccga gcgtaatcag ccaaatcgcg ttaagcggag
    31261 agatttgcac atcgtagctt accagcgaca gcgcaccgct cacgccaacc gcgccagtca
    31321 gtacagtgaa gcccgcggct gccgtataca gactaccaac cgcgccgcca attccggcta
    31381 tccagccgct tagcgccttt tgaaaagaaa agagaaatgc cagaacagcg gcggcgacaa
    31441 accacgccac gccgctattg atcagggaaa ttgcgctcat ttagcctctc cactttgagc
    31501 ctgctgaaac aaggtgagat cgccaaagtc cgtgttaaag gtcagctcac gcttacgttt
    31561 gctgacgcgg gcgatatcgg tgttatccac cagatgcagg gctttagtcg ggcacatccg
    31621 cacgcaggcc ggaccttgtt catcaaagct acaaaggtca catttgacgg cgatcgcgcg
    31681 aatacctggc acccagtcaa gcaatgtgct gacacgcgcc ggagcaggcg gtgccggtgg
    31741 cgctttcggg gtattggcgt ttgccggaat atccagcgga cggctgccgg aaaattcaat
    31801 tgcgccaaac gggcaggcga tgccgcacag tttgcagctt acgcacaggc tttcattcaa
    31861 ctgcacggcc ccatcaacgc gggtgatggc gttaaccggg cagaccaccg cgcagggtgc
    31921 atcttcacag tggtgacaga gctgcggcgc agattctttt tcatttagca tcactctcag
    31981 gcgcggcatt gattgcaggc cgtgctggcg atgcgtctct gaacaggcgg cctcacaagt
    32041 gtggcagccg atacagagcg tggagtcagc aattacaaaa cgattcacca ggcattcctc
    32101 aggtgattgt catttttgac gaaaacatgc cgatgaaatg tcattttcga cactcatcga
    32161 cacgcccatc cccaaacagg cgtaacgcct gcaaaacggg caaagcctca gctcatgctg
    32221 ccgggctttg tccctttacc agttggctta aattcaccgg cacattgttt tcaatagcgg
    32281 tgtataagct gtcgtaaacg ccagcgattt tttcgagata gtgttccaga acctgttcgc
    32341 gatcgcggat gttgaccttg ccatcaatca tgccgtcaat gctcagttgt gcctgcgcaa
    32401 tccgctgttt gtcttcgcca tcttttgttt cgacgtaata ctcgatggtg tggctgttaa
    32461 agccatccgc cagggtgacg taaatacgaa aatgttcaaa aaggacgaaa cagagttctt
    32521 tgtccggcgc gcagctcatg gcgtgatgca ggcgcgcttt cgataacgtg atcccctgaa
    32581 ccagcgaatt gcagtagatg tgccactggt cctgtaggcg acgatgccgc tgtgcgatgt
    32641 aatcggcttt ctcgcttatt tcccaaatag tcattgtcag gttacccgtt taacagagat
    32701 gccagcttta agcatttttt gtgccaactt ttaatttatt gttattaaag agatttttaa
    32761 gctaaagatg aatttcgtcg ccgtgtcgac gtgtcatttc gacatcatcg acattattca
    32821 ccgcagggat aatcaacact ggcacaatta ttgcttgtag ctggcaatag ttaatgggag
    32881 gcgatatgca cgaaataacc ctctgccaac gggcactgga attgatcgaa cagcaggccg
    32941 caaaacacgg cgcaaaacgc gtaactgggg tctggctcaa aattggcgca ttttcttgtg
    33001 tcgaaaccag ctctcttgcc ttttgttttg atctggtttg ccgcggcagc gtggcggaag
    33061 gttgtaaact gcacctcgaa gaacaagagg ccgaatgctg gtgtgaaaca tgccaacagt
    33121 atgtgacgct actgacccag cgcgtccgcc gctgtccaca gtgtcatggt gacatgctac
    33181 agattgtggc agacgacggt ttacagattc ggcggataga aatagaccag gagtgagcga
    33241 tgtgtacaac atgcggttgc ggtgaaggca acctgtatat cgagggtgat gaacataacc
    33301 ctcattccgc gtttcgtagc gcgccatttg ccccggcggc acgcccgaag atgaaaatca
    33361 ccggcattaa agcgcctgaa tttaccccca gccagactga agaaggcgac ctgcattacg
    33421 gtcatggcga agcgggcact cacgcgccgg gcatgagcca gcgccggatg ctggaagtcg
    33481 aaattgacgt gctggacaaa aacaaccgtc tggctgaacg caaccgcgcg cgctttgctg
    33541 cccgcaagca actggtgctc aacctggttt ccagccctgg ttccggtaaa accaccctgc
    33601 tgacggaaac cttaatgcgc ctgaaagaca gcgttccgtg cgcggttatt gaaggcgatc
    33661 agcaaaccgt gaacgatgcc gcacgcattc gcgctaccgg cacaccagcg attcaggtga
    33721 acaccggtaa aggctgccat cttgacgcac agatgattgc cgatgccgca ccgcgtctgc
    33781 cactggacga taacggtatt ctgtttatcg aaaacgttgg caacctcgta tgcccggcca
    33841 gcttcgatct cggtgaaaaa cacaaagtgg cggtgctttc cgttaccgaa ggtgaagaca
    33901 aaccgctgaa atatccgcat atgtttgccg ccgcctcgct gatgctgctc aacaaagttg
    33961 acctgttgcc gtatctcaac tttgacgttg agaagtgcat cgcctgcgcc cgcgaagtca
    34021 atccagaaat tgaaatcatc cttatttccg ccaccagcgg cgaagggatg gaccagtggc
    34081 tgaactggct ggagacacag cgatgtgcat aggcgttccc ggccagatcc gcaccattga
    34141 cggcaaccag gcgaaagtcg acgtctgcgg cattcagcgc gatgtcgatt taacgttagt
    34201 cggcagctgc gatgaaaacg gtcagccgcg cgtgggccag tgggtactgg ttcacgttgg
    34261 ctttgccatg agcgtaatta atgaagccga agcgcgcgac actctcgacg ccttacaaaa
    34321 catgtttgac gttgagccgg atgtcggcgc gctgttgtat ggcgaggaaa aataatgcgt
    34381 tttgttgatg aatatcgcgc gccggaacag gtgatgcagt taattgagca tctgcgcgaa
    34441 cgtgcttcac atctctctta caccgccgaa cgccctctgc ggattatgga agtgtgcggt
    34501 ggtcataccc acgccatttt taaattcggc ctcgaccagt tactgccgga aaacgttgag
    34561 tttatccacg gtccgggttg cccggtgtgc gtactgccga tgggcagaat cgacacctgc
    34621 gtggagattg ccagccatcc ggaagtcatc ttctgtacct ttggcgacgc catgcgcgtg
    34681 ccggggaaac agggatcgct gttgcaggca aaagcacgcg gtgccgatgt gcgcatcgtt
    34741 tactcgccga tggatgcgtt gaaactggcg caggagaatc caacccgcaa agtggtgttc
    34801 ttcggcttag gttttgaaac caccatgccg accaccgcta tcactctgca acaggcgaaa
    34861 gcgcgtgatg tgcagaattt ttacttcttc tgccagcaca ttacgcttat cccgacgctt
    34921 cgcagtttgc tggaacagcc ggataacggt atcgacgcgt tcctcgcgcc gggccacgtt
    34981 agtatggtta tcggcactga tgcctataat tttatcgcca gcgattttca gcgtccgctg
    35041 gtggtcgctg gtttcgaacc ccttgatcta ctgcaaggcg tggtgatgct ggtggagcag
    35101 aaaatagcgg cccacagcaa ggtagagaat cagtatcgtc gggtggtacc ggatgccggt
    35161 aacctgctgg cgcaacaggc gattgccgat gtgttctgtg tcaacggcga cagcgaatgg
    35221 cgcggcttag gcgtgattga atcttctggc gtgcacctga cgccggatta tcaacgattc
    35281 gatgccgaag cacatttccg cccggcaccg cagcaggtct gcgatgaccc gcgcgcgcgt
    35341 tgtggcgaag tcttgacggg caaatgtaag ccgcatcaat gcccgctgtt tggtaacacc
    35401 tgtaatcctc aaaccgcgtt tggtgcgctg atggtttcct ccgaaggagc gtgcgccgcg
    35461 tggtatcagt atcgtcagca ggagagtgaa gcgtgaataa tatccaactc gcccacggca
    35521 gcggcggcca ggcgatgcag caattaatca acagcctgtt tatggaagcc tttgccaacc
    35581 cgtggctggc agagcaggaa gatcaggcac gtcttgatct ggcgcaactg gtagcggaag
    35641 gcgaccgtct ggcgttctcc accgacagtt acgttattga cccgctgttc ttccctggcg
    35701 gtaatatcgg caagctggcg atttgcggca ccgccaatga cgttgcggtc agtggcgcta
    35761 ttccgcgcta tctctcctgt ggctttatcc tcgaagaagg attgccgatg gagacactga
    35821 aagccgtagt gaccagcatg gcagaaaccg cccgcacggc aggcattgcc atcgttactg
    35881 gcgatactaa agtggtgcag cgcggcgcgg cagataaact gtttatcaac accgcgggca
    35941 tgggcgcaat tccggcgaat attcactggg gcgcacagac gctaaccgca ggcgatgtat
    36001 tgctggttag cggtacactc ggcgaccacg gggcgactat ccttaacctg cgtgagcagc
    36061 tggggctgga tggcgaactg gtcagcgact gcgcggtgct gacgccgctt attcagacgc
    36121 tgcgtgacat tcccggcgtg aaagcgctgc gtgatgccac ccgtggtggt gtaaacgcgg
    36181 tggttcatga gttcgcggca gcctgcggtt gcggtattga aatttcagaa gcggcactgc
    36241 cggttaaacc agccgtgcgc ggcgtttgcg aactgctggg actggacgcc ctgaactttg
    36301 ccaacgaagg caaactggtg atcgccgttg aacgcaacgc ggcagagcaa gtgctggcag
    36361 cgttacattc ccatccactg gggaaagacg cggcgctgat tggtgaagtg gtggaacgta
    36421 aaggtgttcg tcttgccggt ctgtatggcg tgaaacgaac cctcgattta ccacacgccg
    36481 aaccgcttcc gcgtatatgc taataaaatt ctaaatctcc tatagttagt caatgacctt
    36541 ttgcaccgct ttgcggtgct ttcctggaag aacaaaatgt catatacacc gatgagtgat
    36601 ctcggacagc aagggttgtt cgacatcact cggacactat tgcagcagcc cgatctggcc
    36661 tcgctgtgtg aggctctttc gcaactggta aagcgttctg cgctcgccga caacgcggct
    36721 attgtgttgt ggcaagcgca gactcaacgt gcgtcttatt acgcatcgcg tgaaaaagac
    36781 acccccatta aatatgaaga cgaaactgtt ctggcacacg gtccggtacg cagcattttg
    36841 tcgcgccctg atacgctgca ttgcagttac gaagaatttt gtgaaacctg gccgcagctg
    36901 gccgcaggtg ggctataccc aaaatttggt cactattgcc tgatgccact ggcggcggaa
    36961 gggcatattt ttggtggctg tgaatttatt cgttatgacg atcgcccctg gagcgaaaaa
    37021 gagttcaatc gtctgcaaac atttacgcag atcgtttctg tcgtcaccga acaaatccag
    37081 agtcgcgtcg ttaacaatgt cgactatgag ttgttatgcc gggaacgcga taacttccgc
    37141 atcctggtcg ccatcaccaa cgcggtgctt tcccgcctgg atatggacga actggtcagc
    37201 gaagtcgcca aagaaatcca ttactatttc gatattgacg atatcagcat tgtcttacgc
    37261 agccaccgta aaaacaaact caacatctac tccactcact atcttgataa acagcatccc
    37321 gcccacgaac agagcgaagt cgatgaagcc ggaaccctca ccgaacgcgt gttcaaaagt
    37381 aaagagatgc tgctgattaa tctccacgag cgggatgatt tagcccccta tgaacgcatg
    37441 ttgttcgata cctggggcaa ccagattcaa accttgtgcc tgttaccgct gatgtctggc
    37501 gacaccatgc tgggcgtgct gaaactggcg caatgtgaag agaaagtgtt taccactacc
    37561 aatctgaatt tactgcgcca gattgccgaa cgtgtggcaa tcgctgtcga taacgccctc
    37621 gcctatcagg aaatccatcg tctgaaagaa cggctggttg atgaaaacct cgccctgacc
    37681 gagcagctca acaatgttga tagtgaattt ggcgagatta ttggccgcag cgaagccatg
    37741 tacagcgtgc ttaaacaagt tgaaatggtg gcgcaaagtg acagtaccgt gctgatcctc
    37801 ggtgaaactg gcacgggtaa agagctgatt gcccgtgcta tccataatct cagtgggcgt
    37861 aataatcgcc gcatggtcaa aatgaactgc gcggcgatgc ctgccggatt gctggagagc
    37921 gatctgtttg gtcatgaacg tggggctttt actggtgcca gcgcccagcg tatcggtcgt
    37981 tttgaactgg cggataaaag ctccctgttc ctcgacgaag tgggcgatat gccactggag
    38041 ttacagccga agttgctgcg tgtattgcag gaacaggagt ttgaacgcct cggcagcaac
    38101 aaaatcattc agacggacgt gcgtctaatc gccgcgacta accgcgatct gaaaaaaatg
    38161 gtcgccgacc gagagttccg tagcgatctc tattaccgcc tgaacgtatt tccgatccac
    38221 ctgccgccac tacgcgagcg tccggaagat attccgctgc tggcgaaagc ctttaccttc
    38281 aaaattgccc gtcgtctggg gcgcaatatc gacagcattc ctgccgagac gctgcgcacc
    38341 ttgagcaata tggagtggcc gggtaacgta cgcgaactgg aaaacgtcat tgagcgcgcg
    38401 gtattgctaa cacgcggcaa cgtgctgcag ctgtcattgc cagatattgc tttaccggag
    38461 cctgaaacgc cgcctgccgc aacggttgtc gctcaggagg gcgaagatga atatcagttg
    38521 attgttcgcg tgctgaaaga aactaacggt gtggttgccg ggcctaaagg cgctgcgcaa
    38581 cgtctggggc tgaaacgcac gaccctgctg tcacggatga agcgactggg aattgataaa
    38641 tcggcattga tttaactgca aattgccgga cagatctgac tgtccggcat actattcacg
    38701 aggttttttc ggacgatatt tttccggcag ttctggcacc ggacgcttgt catcgatgag
    38761 atgacgcgcg gttaagatcg gatgacgcca cagcattctc ggcccggccc aacgcataat
    38821 ctgtttcatc tcttcacgct ttgcaggctg gtaacagtgc accggacact gcttacaggc
    38881 tggtttctct tcgccgaaca cacatttatc cagccgcttt tgcgcgtaag caaacaacgc
    38941 ctcgtaatgc tccggctccg ctgacgcctg cgggcatttc gcttgataaa gatcgatcat
    39001 ttttttaatc gtcagttttt cacgagagat acgcttgccg gacatgctgc ctccacctca
    39061 ttaagatgca tttatattac aacttaatct taaagggcac tatgactcta aagaagaagg
    39121 gttcgccaac cgatacaatt ttgcgtactt gcttcataag catcacgcaa aagctgcaaa
    39181 acagcatctt tcccggaacc agcatcaaga actcgccgtt cgcttcttcc cctgaaatga
    39241 ttaactccgg tatcatgtgc gccttatgtg attacaacga aaataaaaac catcacaccc
    39301 catttaatat cagggaaccg gacataaccc catgagtgca atagaaaatt ttgacgccca
    39361 tacgccgatg atgcagcagt atctcaagct gaaagcccag catccagaga tcctgctgtt
    39421 ctaccggatg ggtgactttt acgaactgtt ttatgacgac gctaaacgcg cgtcgcagct
    39481 gctggatatt tcactgacca aacgcggtgc ttccgcggga gagccgatcc cgatggcggg
    39541 gatcccctac catgcggtgg aaaactatct cgccaaactg gtgaatcagg gcgagtccgt
    39601 tgccatctgc gaacaaattg gcgatccggc gaccagcaaa ggtccggttg agcgcaaagt
    39661 tgtgcgtatc gttacgccag gcaccattag cgatgaagcg ttgttacaag aacgccagga
    39721 caatctgctg gcggctatct ggcaggacag caaaggtttc ggctacgcga cgctggatat
    39781 cagctccggg cgttttcgcc tcagcgaacc ggctgaccgc gaaacgatgg cggcagagct
    39841 gcaacgtacc aacccggcag agctgttgta tgcagaagat ttcgccgaga tgtcgctgat
    39901 tgaaggtcgt cgcggtctgc gtcgtcgtcc gctgtgggag tttgaaatcg acactgcgcg
    39961 ccagcagttg aatctgcaat ttggcacccg cgatctggtc ggttttggcg tcgagaacgc
    40021 gccgcgcgga ctttgtgctg ccggttgtct gttgcagtat gcgaaagata cccaacgcac
    40081 caccctgccg catatccgtt ctatcacgat ggaacgtgag caggacagca tcattatgga
    40141 tgccgcaacg cgtcgtaatc tggaaatcac ccagaatctg gcgggtggcg tggaaaacac
    40201 tctggcttca gtgctcgact gcaccgtcac gccgatgggt agccgtatgc tgaaacgctg
    40261 gctgcatatg ccagtgcgcg acacccgtgt gttacttgag cgccagcaaa ctattggcgc
    40321 attgcaggat ttcaccgccg agttacagcc ggtactgcgt caggtgggcg atctggaacg
    40381 tattctggcg cgtctggcgt tgcgtactgc tcgcccgcgc gatctggccc gtatgcgcca
    40441 tgctttccag caactgccgg aactgcgcgc gcagttagaa aatgtcgata gtgcaccggt
    40501 acaggcgctg cgtgagaaga tgggcgagtt tgccgagctg cgcgatctgc tggatcgagc
    40561 aatcatcgac acaccgccgg tgctggtacg cgacggtggt gttatcgcat cgggctataa
    40621 cgaagagctg gatgagtggc gcgcgctggc tgacggcgcg accgattatc tggagcgtct
    40681 ggaagtccgc gagcgtgaac gtaccggcct ggacacgctg aaagttggct ttaatgcggt
    40741 gcacggctac tacattcaaa tcagccgtgg gcaaagccat ctggcaccca tcaactacat
    40801 gcgtcgccag acgctgaaaa acgccgagcg ctacatcatt ccagagctaa aagagtacga
    40861 agataaagtt ctcacctcaa aaggcaaagc actggcactg gaaaaacagc tttatgaaga
    40921 gttgttcgac ctgctgttgc cgcatctgga agcgttgcaa cagagcgcga gcgcgctggc
    40981 ggaactcgac gtgctggtga acctggcgga acgggcctat accctgaact acacctgccc
    41041 gacctttatt gataaaccgg gcattcgcat taccgaaggc cgccatccgg tggttgaaca
    41101 ggtgctgaac gagccattta tcgccaaccc gctgaatctg tcaccgcagc gccggatgtt
    41161 gattattacc ggcccgaaca tgggcggtaa aagtacctat atgcgccaga ccgcactgat
    41221 tgcgctgatg gcctacatcg gcagctatgt accggcacaa aaagtcgaga ttggacctat
    41281 cgatcgcatc tttacccgcg taggcgcggc agatgacctg gcttccgggc gctcaacctt
    41341 tatggtggag atgactgaaa ccgccaatat tttacataac gccaccgaat acagtctggt
    41401 gttaatggat gagatcgggc gtggaacgtc cacttacgat ggtctgtcac tggcatgggc
    41461 gtgcgcggaa aatctggcga ataagattaa ggcattgacg ctgtttgcta cccactattt
    41521 cgagctgacc cagttaccgg agaaaatgga aggcgtcgcc aacgtgcatc tcgatgcact
    41581 ggagcacggc gacaccattg cctttatgca tagcgtgcag gatggcgcgg cgagcaaaag
    41641 ctacggcctg gcggttgcag ctctggccgg cgtgccaaaa gaggtgatca aacgcgcgcg
    41701 gcagaaactg cgtgagctgg aaagcatttc accgaacgcc gccgctacgc aagtggatgg
    41761 tacacaaatg tctttgctgt ccgtaccgga agaaacctcg cctgcggtcg aggcactgga
    41821 aaacctcgat ccagattcac tcaccccgcg tcaggcgctg gaatggattt atcgcctgaa
    41881 gagtctggtg taataataat tcccgatagt cttttgctat cgggaatatt aacgataact
    41941 gacgaatcaa ataaaaatac cctgtataat aggaaagctt attttacagg gtaaaaccat
    42001 gccatctaca cgctatcaaa aaatcaatgc ccatcactat cgccatatat gggtcgttgg
    42061 tgatattcat ggtgaatatc agttattaca atcccgctta catcaactct ctttttgccc
    42121 tgaaacagac ttacttattt ctgtcggcga taacattgat cgtgggccgg agagtcttaa
    42181 cgtgctacgc ctgttaaacc agccctggtt tacatcggtt aaaggcaacc atgaagcgat
    42241 ggcgcttgat gcattcgaaa ctggcgatgg aaatatgtgg cttgccagcg gtggtgactg
    42301 gtttttcgat ttaaatgatt cagagcaaaa agaagctata agtctgttgc tgaaatttca
    42361 ttaccttcca catattattg aaattaccaa cgacatcata aaatatgtca tagcacatgc
    42421 ggattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg aattactctg
    42481 gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa acggcgctga
    42541 ttattttata tttggacata tgatgtttga taacattcag acgttcgcta accagattta
    42601 tattgatacc ggatcgccga aaagcgggcg gctgtcattt tataaaataa agtaatgagg
    42661 ccgggtaaag caaagccact acccggcact ttttattagc gcttaccttc cgccagcagc
    42721 ggcgggatgc tcggcaccat tggcgcgtca tcaatatctt tctgcgtcat gcggaacgct
    42781 tcgggataat gttcgcggct ggtacggcgc agcggttcgg tatcgcgcca ggtataaagg
    42841 caatgctggc actgatatac cgtccagaca tctttcaccg gcgatttcgc catcacttca
    42901 atctgttcat cggcacaacg tggacaaatc atcttgttct ccttatttac gtgcagccag
    42961 catagcggtc agtttttcag cccaggcttt ggtttccggc aaatccacca ccggctggct
    43021 gtagtgacca cggttgtccg gggcgacagg cgtggtggca tcgataatca gcttgtcggt
    43081 gatccccgcc gggcttgagc cagggtcgag ttccagcacg gacatattcg gcaactgcac
    43141 caaatcccct gccggattta ctttcgagga gagcgcccac atcacctgcg gcaggttgaa
    43201 cgggtcaacg tcttcatcga ccataatcac catctttacg tagcccagac cgtgcggcgt
    43261 ggtcatcgca cgcaggccca ccgcgcgggc aaagccgccg tagcgttttt tggtggagat
    43321 aatcgccagc aggccgtggg tgtacatggc gtttaccgcc tgcacttccg ggaactcggc
    43381 tttcagttgt tgatacagcg gtacgcaggt ggctggcccc atcaggtagt cgatttcggt
    43441 ccacggcatg ccgaggtaca gcgattcgaa aatcggcctg gtgcggtaag agactttatc
    43501 gatacgcacc acggtcatgt tacgcccgcc ggagtagtgc ccggtaaact caccgaacgg
    43561 cccctcgatt tcgcgtttac ggctttcgat gaccccttcg aggatcactt ctgaacccca
    43621 cggcacatca aaaccggtca gcggggcggt ggcgatcggg tacgggcttt cacgcagcgc
    43681 gcctgccatt tcatactcag actgatcgta tttcagcggc gtggccccca tcagcgtgat
    43741 gatcggatcg ttaccgagcg tgatcgcaat cggcagatct tcaccgcgct cttctgcttt
    43801 atgcagatgc agggcgatat cgtgcatcgg caccggttgc aggccgagct tacgcttgcc
    43861 cttcacttcc atgcggtaga tgccgacgtt ctgcttgccg aagttatctg ggtcgagcgg
    43921 atcgcgggaa accacgcacg ctttgtcgag atagaaaccg ccatcaccgt cgtttaaacg
    43981 aaacagcggc aggatatcga acagattaat ctcgtcacca tcgacggtgt tctgcgccca
    44041 ggctggattg gcgcggcgct ccggggcgat cgggaagtta tcccagcggc ggataaactc
    44101 atcaatctgt tttttaaccg gggtgtttgg cggcaggccc agggaaatcg cgtggttctg
    44161 ccaggaaccg atggtattca tcgccacgcg ggcatcggta aaaccgcgaa tattatcaaa
    44221 ccacagcgcc ggtgcaccat cgccgatacg cccggtggcg ttggcagcgg cagccagatc
    44281 cggttccgcg ttcacctctt cgctgatttt cagtaactgg ccgtggtcat caagcgcctg
    44341 taaaaagctg cgtaaatcat caaatgccat tattcattct cctgagaaaa attccggacc
    44401 tgcggcaatc cttgccagcg cctggcgtgg ggatgttcga ggccaaattg atccagcacg
    44461 cgggctacta catggtggac aatgtcatct accgtttcgg gatggttata aaaggcaggc
    44521 atcggcggca ccatcgccac gcccatgcgt gaaagtgcga gcatattttc gagatggatg
    44581 gtgctaagcg gcatttcacg cggcaccagc accagtttgc ggccttcttt gagcacgacg
    44641 tccgccgcgc gccctaccag gccatcagcg taaccagcgc ggataccggc gagcgttttc
    44701 atctgcacgg aataacgatc atgccgtcgg tacgaaagga acctgaggag atggtcgccg
    44761 cctgatccgc cgggttatgg ctgaagtcag cgagggcagc aacatcgcgg gcgctgtaag
    44821 gcgtttccag ttcaatggtg gttttcgccc acttcgacat caccagatga gtctcgacat
    44881 tcggcatctc ccgcagcgct tgcagtaatg ccacaccaag aggcgcaccg gtagcccctg
    44941 tcatcccgac gatcagtttc attgcttact ctcttaattg ttcgtatacg aacattatta
    45001 ttaaaatacg cctctgttct cattgcggca agcactgaaa agtaacaaca tccccttcgt
    45061 cactgaaaaa ccgctatgat agcggcagat agtttgcccg gagtttacat ggcgttacga
    45121 aataaagcgt tccatcagtt acgacagctt tttcagcagc acacggctcg ctggcagcac
    45181 gagttacctg acctgaccaa accacagtat gcggtgatgc gcgccattgc tgataagcct
    45241 ggtatcgaac aggtggcact gatagaagca gcagtcagca ccaaagcaac gttggcagaa
    45301 atgttggcaa gaatggagaa tcgtggctta gtcagacgtg aacatgatgc cgctgacaag
    45361 cggcgtcgct ttgtctggct gactgccgaa ggagaaaaga tacttgctgc ggcgataccg
    45421 attggtgaca gcgtggatga ggaatttttg gggcgtttga gtgcagaaga gcaggagctg
    45481 tttgtgcagc tggtgcgcaa gatgatgaac acataggatg caaattgccg ggtaggacgc
    45541 tgacgtgtct tatccaggcg acaaaaacac aagcttccca aacaaaagaa aaaggccagc
    45601 ctcgcttgag actggccttt ctgacagatg cttacttatt cgcggaacag cgcttcgata
    45661 ttcagcccct gcgtttgcag gatttcgcgc aaacggcgca ggccttcaac ctgaatctgg
    45721 cgaacacgtt cacgggtgag gccaatttca cgacctacat cttccagtgt tgccgcttcg
    45781 taccccagca aaccgaatcg acgtgccagt acttcacgct gtttggcgtt cagctcgaac
    45841 agccatttga cgatgctctg cttcatatcg tcatcttgcg tggtatcttc cggaccgttc
    45901 tctttttcat cggccaggat gtccagcaac gctttttcgg aatcaccacc cagcggggtg
    45961 tctaccgagg taatgcgctc gttaagacga agcatacggc tgacgtcatc aactggctta
    46021 tccagttgct ctgcgatctc ttccgcactt ggttcatggt ccagcttatg ggacaactca
    46081 cgtgcggttc gcaggtaaac gttcagctcc tttacgatgt gaatcggcaa acgaatagta
    46141 cgggtttggt tcataatcgc ccgttcaatc gtctggcgaa tccaccaggt tgcgtatgtt
    46201 gagaagcgga aaccacgttc cgggtcaaac ttctctaccg cgcggatcag ccccaggttg
    46261 ccctcttcga taaggtccag caacgccaga ccacgattac cataacggcg ggcaattttt
    46321 accaccagac gcaagttact ctcgatcatc cggcggcgag aggcgacatc tccacgcagt
    46381 gcgcgacgcg caaaataaac ttcttcttcg gccgttaaca gtggtgaata accaatctca
    46441 ccaaggtaaa gctgagtcgc gtccaacaca cgctgtgtgg ctccctgcga taacagttcc
    46501 tcttcggcca aatcgttatc actgggttcc tcttctacta aggccttttc gtcaaaaacc
    46561 tcaactccgt tctcatcaaa ttccgcatct tcatttaaat catgaacttt cagcgtattc
    46621 tgactcataa ggtggctcct acccgtgatc ccttgacgga acattcaagc aaaagcctgg
    46681 ttccgccgat ttatcgctgc ggcaaataac gcagcgggtt tacggatttc cccttgtaac
    46741 gaatttcaaa atgcaagcgt gttgaactgg ttccggtgct acccatggtt gctatttttt
    46801 gccccgcctt cacttcttgt tgttcccgga ccagcattgt gtcgttatgg gcgtaggcac
    46861 tcaggtaatc atcattatgt ttgatgataa tcagattacc gtagccgcgc agcgcgttac
    46921 cagcataaac aacgcggcca tctgcggtcg cgataattgc ctgtcctttg ctgcctgcga
    46981 tatcaatccc cttgttgccc ccctcagaag cgccaaaggt ttcgatcact ttgccctcag
    47041 tcggccagcg ccaggtggag ataggcgtac tggttgatgt actgctgaca gtcggctcgg
    47101 ttgtgcttgc tgttggtacc gttacaggcg ctgtgaccgt ggtcgcagtt ggcttgttgt
    47161 tcggcaacat tttgttagca ctctgttcac ccgaagactc agaatacgta attgtcggtt
    47221 gcgacgcaac agcaacggtg gaattttgtg caggcttgat cacaactcct tgctctgctg
    47281 cgtcggcctg ggtaatggca tttccgccag tgattggcgt accggaagca ttccccacct
    47341 gtaaggtctg accaacgttc agcgcgtatg gtgcctgaat attgttgcgc tgagcaaggt
    47401 cacggaaatc gttgccagta atccaggcga tatagaaaag tgtgtcgcct tttttcacgg
    47461 tgtatgtact gccgctataa ctgcctttcg gaatgttccc atactgacgg ttatagacga
    47521 tgcgtccgtt ttccatctgt accggctgct gagccactgg ctgcactggc tggatttgcg
    47581 gttgttgagt ggcctgaatt tgtggctgct gtaccggctg aatttgcggt tgctgggctg
    47641 tagacgtcgt ccccattttc ggcggcggcg taatcaacat accagaattg gtatttgcag
    47701 gcgcattgcc attaacggag ctgaccggtg ccggtggatt tgaagtgtca gaacagcctg
    47761 ccagccatag cgaaaccagt gacaaagccg caatgcggcg aacggtgaat tttgggcttc
    47821 ccgcgctcat ttatccccca ggaaaaattg gttaataacc agtgacataa ttaccgtgca
    47881 aggcaccata ctgaacactg gaaaagatgt tcacgatacg ctgacctgcg gcaaaataac
    47941 caggaaaaat ccaggtattt cctcacgttt taagccagct cacccttcac taaagggaca
    48001 aagcgcacgg cctccacggt atcgataata aattcgcctc cccgacgacg cacccgtttc
    48061 aaatactggt gctcctcccc tacgggtaag acgagaatcc cgccttcgtc cagctgcgtc
    48121 attagcgcag ttggaatttc cggcggtgcc gccgtaacaa tgatagcgtc aaacggcgca
    48181 cgtgcctgcc aaccttgcca tccatcgcca tgacgggttg aaacattatg taaatcaaga
    48241 tttttcaggc ggcgacgcgc ctgccactgc aaacctttaa tccgttcgac cgagcaaaca
    48301 tgctggacaa gatgcgccag gattgccgtt tgatatcccg aaccggtgcc aatttccagc
    48361 acccgcgact gcggcgtcag ctcgagtaat tcggtcattc gcgccaccat atacggctgc
    48421 gaaattgtct gcccctgacc tatcggcaag gcgatattgt cccaggcttt ttgttcaaac
    48481 gcttcatcaa cgaatttttc acgcggcacg gcagcaagtg cattcagcac ctgctcatcc
    48541 tgaatacctt gcgcacgtaa ttgatccaga agtgcttgta cgcgtctgct taccattgcg
    48601 tgccaactcc cacgctgttt aaccagtctg aaaccacatc ttgcgcgcta tgcgcagtta
    48661 aatccacatg cagcggcgtg atggaaacat agccctcatc taccgcagca aaatcggtcc
    48721 ccggaccggc atcacattta ccgcccggcg ggccaatcca gtacagcgta ttgccgcgcg
    48781 gatcttgctg cgggatcacc tgatctgccg gatgtcgtgt accgcagcgc gtcacgcgaa
    48841 tacctttgat ttgatccaag ggtaaatccg gaacgttaat attaagaata cgcccggtgc
    48901 gcagcggctc tttacacagt gcgcgcaaaa ttgaacaggt taccgccgcg gcagtgtcgt
    48961 aatgattatg cccgtcaagc gagacggcaa gcgccggaaa acctaaatga cggccttcca
    49021 tcgcggcggc taccgtaccg gaataaataa catcatcccc cagattcggc ccggcgttaa
    49081 ttccggacac aacaatgtcc gggcgcggac gcatcagagc attcacgcca agatagacgc
    49141 aatcggtcgg ggttcccatt tgcacagcaa tatcaccatt ttcaaaggta aacgtgcgca
    49201 gggaggattc cagtgtcaga gaatttgaag cgccgctgcg gttacgatcg ggggcgacca
    49261 cctgaacgtc agcaaactca cgcaaggctt tcgccagcgt ttgtataccg ggtgcatgta
    49321 ccccgtcatc attactcagc aatatgcgca taatcacctg ttgtgttgat aagttccctg
    49381 acaacgctgg ttgcaaaact acccgccgga agccagaaac ggatttctac ggtgacgtca
    49441 tcccaccaat tccagcttaa ttgttgcgga tacagcagca tcgctctgcg cgcggcttca
    49501 actttttcgc gcaccagtaa agcttgtaat tcagtttctg cggcgacagc tgcttgttcg
    49561 aatgccagcg cttcacgctg agttccccat tcgccactgc ccggcaatgc ggcggttatc
    49621 atcagctctt tatcgttgac gcgacgctgt aattccgcca gttcttcggt ggttgctaca
    49681 aaccagctac cacgtccggc taattgtagc gcatcgccgt caacaacttg attaacgtct
    49741 gcttttttga ggcgctcagc aacaatctga ttaaacaacg cactgcgggc tgccgacaac
    49801 caaaaactcc gtttattgcg atcgcgcacc ggagtattgg tttgcgccca gcgcagcgcg
    49861 ccctgcaagt tgctaccgcc aatcccaaaa cgttgggcac cgaagtagtt cggtacacct
    49921 tttacgcaaa tatcgatcag acgttgttca acgtcatcgc gattgctcac ttcgcgcaga
    49981 accagggtaa aggcgttacc tttcagcgcc cctaaacgca gcttgcgctt gtgccgcgca
    50041 tactccagca cctggcagcc ttccagttga aaggcgctca gatcgggcat ttccttgccc
    50101 ggcacgcgag cacataacca ctgttccgtt acagcatgtt tgtctttttg cccagcgaag
    50161 ctgacttcac gggaatgaat tttcaggaat ttcgccagtg catccgccac aaaacgggta
    50221 ttgcagccgt ttttgaggat tctaaccaga atatgctcac cttcaccatc aggctcaaag
    50281 cccaaatctt ccaccaccac aaagtcttcc ggattggctt tcagcagccc ggtgccttgc
    50341 ggtttaccgt ggaggtaagt gagattatca aactcaatca ttttgttgcc ttaatgagta
    50401 gcgccaccgc ttcacaggca atcccttccc cacgtccggt aaatccaagt ttttccgtag
    50461 tagtggcttt cacgttaaca tcatccatat ggcagccgag atcttcggca ataaacacgc
    50521 gcatttgtgg aatgtgcggc aacatcttcg gtgcctgagc gatgatagtg acatcgacgt
    50581 tgccaagggt ataacccttc gcctgaatac gacgccaggc ttcgcgtagc agctcgcggc
    50641 tgtcggcacc tttaaatgcc ggatcggtat ccgggaacag cttgccgata tcccccagcg
    50701 ccgccgcgcc aagcaatgca tcggtcaacg catggagcgc cacgtcgcca tcagaatgcg
    50761 ccagcaatcc tttttcgtaa ggaatgcgta cgccaccaat gataattggg ccttcaccgc
    50821 caaaggcgtg tacgtcaaaa ccgtgtccaa ttcgcattat gtattctcct gatggatggt
    50881 tcgggtgagg taaaactcgg ccagtgccaa atcttccggg cgcgtgactt taatgttatc
    50941 cgcgcggcct tcgaccaact gcggatgaaa tccgcaatat tccagcgccg aggcttcgtc
    51001 ggtaatagtc gcgccttcat ttagagcgcg cgtcagacag tcatgtaaca gctcacgagg
    51061 gaaaaattgc ggcgtcagcg cgtgccataa gccgttgcga tcaacggtat gagcaatggc
    51121 atttttgccc ggttcggcac gtttcatagt atcgcgcact ggtgcggcta ggatccctcc
    51181 cgtgcggctg gtttcgctca acgccaacaa tcgcgcgagg tcatcctgat gcagacaagg
    51241 acgagcggcg tcatgcacca atacccactg cgcgtcgcca gcggctttca gacctgccag
    51301 cacggaatcg gcacgctcat caccgccatc tacaacggtg atttgcggat gattcgccag
    51361 aggaatttgt gcaaaacggc tatcgccagg acttatggca atgacgacac gtttcacccg
    51421 gggatgcgcc agcagcgcat gcaccgagtg ttcaagaatg gtttgattac cgattgagag
    51481 atattgctta ggacattccg tttgcattcg acggccaaat ccggccgccg gaaccacggc
    51541 gcaaacatcc aaatgagtgg ttgccatgtt aattcccggg ctgatttatc gattgttttg
    51601 ccccgcagac tgtgcgcgct tcgacgcgtc aggcaccaga cgataaaaag tttcgcccgg
    51661 cctggtcatg ctgagttcat tacgcgcacg ctcttcgagc gcctcctggc cgccattgag
    51721 atcgtcaatt tcggcaaaaa gttgatcgtt tcgcgcttta agtttcgcgt ttgtagcttg
    51781 ctgtgccgcc acatcatcat tgacgcgggt atagtcatgt ataccgttct taccgaacca
    51841 cagcgaatac tgtagccaga ccagaatagc cagcaacagc agcgttagtt tacccatcct
    51901 gccccctgaa aaacggcatc atcatcccat gcatccgaag acgactctac atcctctgtt
    51961 ggggataccg cgacaacgcg ggcaaatgta ccacatttgt ccattgttac gtatacccag
    52021 ggcgtgcaga acataatctc attattagtt acggtttgaa ttatgaacag aggagacggg
    52081 aaagtacaaa ttagcccagt agccacataa acagtgcgcc aaacataatg cctactgtca
    52141 ccagggtgaa aacaatactg tagcgtagct ttccgtccat caatgaatgc agcgcaatcc
    52201 ccaccactac cgcaacgggc atcagcgcca gaaagaaagg ccaggtgtag ataaagaaga
    52261 acagcgtgtt agagccataa atcaacatcg gcatcgccag cgcaaataac caggagataa
    52321 aaccgaccac ggcaccaggc agtgaccatg tggtttcttc atcctcagta aggctgtcgt
    52381 tatttgttag tgtaatgtta tggctattac gcatatttga tcctgttact ttgacgaacc
    52441 gggcatggaa acccggtggt gtctcaggat ctgataatat cgttctgtct caacagatct
    52501 aataattgct gtaccaaatt tgttactaat tgttcaccat tgagatgaat ttctgccgat
    52561 tcaggcgctt cgtaaacgga atctattccc gtaaagttgc gcagttcacc ggcacgcgct
    52621 ttcttatata agccttttgg atcgcgggct tcgcaaatcg ccagcggcgt atcgacaaac
    52681 acttcgataa agcgcccttc tcctacgcgt tcgcgaacca tctggcgttc ggcgcggtgt
    52741 ggcgagataa atgcggtcag caccaccagt ccggcttcaa ccatcaaatt cgccacttca
    52801 ccgacgcgac ggatattctc tttacgatcg gcatcgctaa aaccgagatc gctgcataat
    52861 ccgtggcgaa cattatcgcc atccagcaga tacgtactga cgccgagttt atgtaacgcc
    52921 tcctccagcg ccccggcaac cgttgattta ccggacccgg agaggccggt aaaccacaga
    52981 actacaccac gatgaccgtg gtgtagctcg cgttgttgca cagtgaccgg atggctatgc
    53041 cagacgacgt tttcgtcatg cagcgccatt atttatcccc cagcaaatcg cgcgcgcccc
    53101 agtgtggaaa gtggcggcga accagggcat tcaattccag ttcgaatgca ctaaattcag
    53161 atggcgcagc agttgcctgg ctaactggct catgcaccat accggcacct acggtcacat
    53221 tgctcaggcg atcgataaaa atcagcccac cggtaaccgg gttttgctga taacgatcta
    53281 acaccagtgg ctcgtcaaaa gtgagatcca ccaggccgat gccgttcagc ggcaggtttt
    53341 caacttcgcg ttgggtaagg ttattaatat caacctgata acgaatgcca tcaacacgag
    53401 cacgcgtctt cttaccggca attttgatgt cataactctg gcccggggaa agcggctgtt
    53461 ccgccatcca taccacatcc accgacgcgc tctgcacagc tggtaacgct tcgtctgccg
    53521 ccagcagcag atcgccacgg ctgatgtcga tctcatccgt cagcaccagg gtgatcgctt
    53581 ctccggcaaa ggcttcttcg cgatcaccat caaaagtcac gatccgcgcg acgtttgatt
    53641 ccacaccaga gggcagcact tttacacgtt gcccgacttc cacgcgaccg gatgccagcg
    53701 ttccggcgta gccacgaaaa tcgagattcg ggcggttaac atactgcacc gggaagcgca
    53761 ttggctgggc atccaccact cgctggatct ccacggtttc cagcacttcg agcagtgttg
    53821 gaccgctgta ccacggcata ctttcacttt gcgaagccac gttgtcgcct tccagcgcag
    53881 agagcggcac aaagcgaata tccagattgc caggtaactg ttcggcgaaa gtcaggtagt
    53941 cttcacgaat gcgggtgaat gtctcttcac tgtaatccac cagatccatt ttgttgatcg
    54001 ctacgaccag atgtttgatc cccagcagcg tggagataaa actgtgacga cgggtttgat
    54061 cgagcacgcc tttacgggcg tcgatcaata agatcgccag ctcacacgtt gaggcgccgg
    54121 tcgccatatt gcgggtatac tgctcgtgcc cgggggtgtc ggcgataata aatttacgct
    54181 tctcggtaga gaaataacgg taagccacgt cgatagtaat accctgttcg cgttcagctt
    54241 gcaggccatc caccagcaac gccaaatcca gtttctcgcc ctgggtaccg tgtcgtttgc
    54301 tgtcgttatg cagcgatgag agctgatctt catagatttg gcgggtatcg tgcagcagac
    54361 gaccaatcag ggtacttttg ccgtcatcga cgctaccaca ggtcagaaaa cgcagcaggc
    54421 ttttatgttg ttgcgcaatc atccaggctt cgacgccgcc ttcattggcg atttgttgtg
    54481 caagtgcggt gttcatctta aaaatacccc tgacgttttt tcagctccat agaaccagcc
    54541 tggtcgcggt caatcacgcg cccctgacgt tcactggtgg tggaaaccag catctcttcg
    54601 atgatctccg gcagcgtttg tgcatttgac tccaccgcac cggtcagcgg ccagcagccc
    54661 agcgtacgga aacgcaccat ccgtttttta atcacttcgc ccggttgcag gtcgatacgg
    54721 ttgtcatcaa tcatcatcaa cataccgtcg cgttccagaa ccggacgttc cgcagcgaga
    54781 tacaacggaa caatgtcgat attttccagc cagatgtatt gccagatatc ctgctcggtc
    54841 cagttagaga gcgggaaaac gcggatgctt tcgcctttgt taatctgccc gttatagttg
    54901 tgccacagct ccgggcgctg atttttcggg tcccagcgat ggaagcgatc acggaaagag
    54961 tagatacgct ctttagcacg ggatttctct tcgtcgcggc gcgcaccacc gaaggcggca
    55021 tcaaaaccgt atttattcag cgcctgcttc aggccttcgg tcttcataat atcggtatgt
    55081 ttcgcgctgc cgtgcacgaa tggattaatc cccatcgcca cgccttccgg gtttttatgc
    55141 accagcagct cgcagccgta ggctttcgcc gtacgatcgc ggaactcata catctcgcgg
    55201 aatttccagc cggtatcgac atgcagcaac gggaaaggca gcgtacctgg ataaaacgcc
    55261 ttgcgcgcca gatgcagcat gacgctggaa tctttaccga tagagtagag catcaccgga
    55321 tttgagaatt ctgccgccac ctcgcgaata atgtggatgc tttccgcctc cagttgccgc
    55381 aggtgagtaa gtcgtatttg atccataacc gttcctttgc aataccgcta ttttcttgcc
    55441 atcagatgtt tcgactatag ggagcgtaag agaacgaatg aaattaccaa ttagaatgag
    55501 tagttcctta acggaataac gatttggcaa agctaatatc aaaaagtgct taaggcaccg
    55561 gatttcgggc gtttaggaag atttgaaatt gttttagcgc agcggcagtt tcatactatg
    55621 gcggtaaaaa aatttgcatg gtatttaagg actcactatg ttttccgcat tgcgccaccg
    55681 taccgctgcc ctggcgctcg gcgtatgctt tattctcccc gtacacgcct cgtcacctaa
    55741 acctggcgat tttgctaata ctcaggcacg acatattgct actttctttc cgggacgcat
    55801 gaccggaact cctgcagaaa tgttatctgc cgattatatt cgccaacagt ttcagcaaat
    55861 gggttatcgc agtgatattc ggacatttaa tagtcggtat atttataccg cccgcgataa
    55921 tcgtaagagc tggcataacg tgacgggaag tacggtgatt gccgctcatg aaggcaaagc
    55981 gccgcagcag atcatcatta tggcgcatct ggatacttac gccccgctga gcgatgctga
    56041 cgccgatgcc aatctcggcg ggctgacgtt acaaggaatg gatgataacg ccgcaggttt
    56101 aggtgtcatg ctggaactgg cagaacgcct gaaaaatacg cctaccgagt atggtattcg
    56161 atttgtggcg accagcggcg aagaggaagg gaaattaggc gctgagaatt tactcaagcg
    56221 gatgagtgac accgaaaaga aaaatacttt attggtgatt aatctcgata atctgatcgt
    56281 tggcgataag ctgtatttca acagtggcgt aaaaacacct gaagcagtaa ggaaattaac
    56341 gcgcgaccgg gcgctggcaa ttgcccgcag tcacggaatt gccgcaacga ccaatccggg
    56401 tttgaataaa aattatccga aaggcactgg gtgttgtaat gacgcagaaa tattcgacaa
    56461 agcgggcatt gctgtacttt cggtggaagc gactaactgg aatcttggga ataaggatgg
    56521 ttatcagcaa cgcgcaaaaa cagccgcatt ccctgcggga aatagctggc atgacgtaag
    56581 actggataat caacagcata ttgataaggc acttcctgga agaatagaac gtcgctgccg
    56641 tgatgttatg cggataatgc taccgttggt gaaggagctg gcgaaggcgt cttgatgggt
    56701 tggaaaatgg gagctgggtg ttctaccgca ggggcgggga attctaagtg atatccatca
    56761 tcgcatccag tgcgcccggt ttatccccgc tggcgcgggg aacactttat acacggatcc
    56821 tgtgtgccgt ggaccgccgg tttatccccg ctggcgcggg gaacaccaca aaccgcccat
    56881 cttcccgatt actgcagccg gtttatcccc gctggcgcgg ggaacaccta atgatgccac
    56941 ccagcgacaa aaacgccgcc ggtttatccc cgctggcgcg gggaacaccc acctgccgcg
    57001 acgggtgcca gcagatcacg cggtttatcc ccgctggcgc ggggaacaca cacccaaaca
    57061 caagcccaaa tcgcgaccga acggtttatc cccgctggcg cggggaacac gatcgcctgc
    57121 tccaacgtca gtccctgccc gtcggtttat ccccgctggc gcggggaaca cactaagcat
    57181 acatatctgt ttttaaacaa atttattcca catcaacaat ctaccaacta aattcaaaca
    57241 tttccttatt tttaaagaac acataaccta ttgattatca acaggaagaa aagaaaccaa
    57301 acgtaaccca tccaaatcca ccggaatacg tctgttttct ccccaggtct gaaattcaaa
    57361 acccgactcg gtattggtcg cccaggccat caccacattt ccgcaaccag ccagttgggt
    57421 aatttgctgc cagatcatct cccgaatacg ttttgatgta tcaccaacat acacaccggc
    57481 acgcacttcc agtagccaga ttgcgagccg tccacgtaag cgcggaggga cattttctgt
    57541 aacaaccacg accatgctca tccgccgcgc ccccggtgac cactatcacc cagcgtttca
    57601 ggttcaggga tggcaggcgg taacatatcc ggcgcgggtt gtggtggttc aatttcacct
    57661 gcagcaagga cttcctcaat taacggtatt aatttgcccg ttaacttagt gctacggaaa
    57721 atatcgcgac aggctaatct gacttcttta tcagattctg ccgggtgtct tgccgctatt
    57781 tcaaatgcct ttggcacaac cgaatcaaat ttaatgatat cggctatgtc ataaacaaat
    57841 gaaagcggtt tgccactatg aataaatcca atagcgggcg catatcccgc ggctaatact
    57901 gccgcttcag aaataccgta cagacatgat gtggcagcac tgatgcagcg attcacaaca
    57961 tcgccttttt cccagtcttt aggatcgtat ttgcgtccat tccatttcac accatattgt
    58021 ttcgccagta atgcataggt ctggcgaacg cgggatccct caattccccg tagctgatcc
    58081 actgaacggc gagctggcgg tggctcacga aaacgtaatt catacatttt gcgcaccacc
    58141 ttcaggcgta gatcttccgt taaagccagc tttgcctggt agagtaattt atctgcccgc
    58201 gcccctccgg gttgtccgga agagtaaacg cgaacgcccg cttcaccgac ccagaccagc
    58261 agtgttccca ccgtggcggc cagatgcacc gccgcgtggg aaactctcgt tcccggttcg
    58321 agcataatgc aggcgaccga tcccaccgga atgtgcgtgc ggatcccggt tttgtcgatc
    58381 agcacgaaag cgccgtccag tacgtcgatt tgaccgtact ggaggaagat catagaggtg
    58441 cgatctttta acgggatcgg actcagtggt acaaacgtca cgcctctgct ccgggtttga
    58501 tcagcatcag accacagccg aacgcgcggc ttttgccata cccctggctg agacgttgca
    58561 gaaataaccc gggatcggtg accgtaagca tccccgtata atccacgcta ctgaactgga
    58621 tcagttgccg ggagttttcc cgccgcagtt gctgttgtcg gtaggcatca accgaggtat
    58681 ccagcagtgt aaaaccgctt ctctccccct gcgctgccag ccagtccagc gccgcctgtt
    58741 gttgatgcaa ccagacatca cgcccttccg cctgtcccct cacctgccgt ttcgcctcca
    58801 tcagcagatc gtggcgcttg cccgctttac aaattgtggg attcgcccgc aggttgaaac
    58861 aaagggattg tccggtacgc agctccggag caaatgaccg gcattcgata gtgaaagttt
    58921 cactttccgc cgggcgttcc tgcgagagga caaaaaagcg aaacgcaccc tgaagttcct
    58981 cccgacgata aagaaattgc ctttctttgc cgccagggaa gagatcccac agccactgat
    59041 gcatcacata ttcaccgcga tccaccagat gcagcaactg cgcaggcaaa agctgagcgg
    59101 tatgtaaggt tattcttgag aggtacatgg ttcctccttg ctgagccacg gcccctgatt
    59161 gacggtgcgt tccccaaaca gccactgctg gcgatttaaa ggaacatccc ggcggcgtaa
    59221 tattttgctc acgaccaggc cgtcgtgctc cccttcccac cagcattcat cctgaagttt
    59281 cgggagtgag actttcaggt cgtggaaatg atcctgatac tgttggtatg cgttacgtaa
    59341 aacatcagac gccttgcctt cgagcagtaa cggcgcaagt ggtaatgcca gcggatgact
    59401 ttttcgcccc agataaagag gaaaaactgg atggcgtaaa ccgccctgca actgttcgag
    59461 gctgtaaggc gcatcggctg ttgttgccac cgccaccatc caccaggcgt cggtgtagta
    59521 gtcgcgccgg gagataatcg cgctcagaag atcagagtcg ctcaactctt cgcgacggct
    59581 gaaataacgc gctttgcgca cctcttttgg catctggatc gtgtgataat cccgtgccca
    59641 gcgcgggtta cggctggcgc aaaccaccag tgaatagtgg cgattaaacg cgtttaagcg
    59701 ttcggtatca tcacgccgaa tccctacccc ggcagccagc agacccagca atgctgagcg
    59761 cgaaggcagt tcatgggtat gacgcacttc gccgggcatc gacgccccag gatgccattg
    59821 gcccatgaag ctgaaaaatc agatattggc tcataagccg ccccttacgc gcagataaag
    59881 tccagtacgt ccttcatgct tccctgtttg ttcatcacgt caaaacttgc gcattcagtc
    59941 ttctgttcgt agaccgtatt catgttttcg cgaagcgttg taatacgctg caccgccaca
    60001 tctaactgac gggtaccatt aatgggttca tagaaagccg ccgccagaga acgcggttga
    60061 tcagtgcctt tttccgccag cgcccaggag gcgtaggcac ggctggcaaa gctattttgt
    60121 ttaccggttg gggaaacttt aagcgcggct tccgtaaaag cgcgtatggt ctgattagct
    60181 aacgcttcgt caccgccgag gttttctacc agcagatctt tatcaatgca gatataggtg
    60241 tagaaaagtg ctgaaccgaa tccggtttct ccgagatgcc cggcaccggc atcttcagag
    60301 gcctggcgca aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt ttcactgaca
    60361 ccaaacgcat gcgcgacctg gcaggcggct tcaacattaa actcaggttt attcgccagc
    60421 atacaaccaa acatagcgat atctaccgct atacggtctt tacgtaacaa cgcgagatct
    60481 tcctcttttg gcgcgcgctt ttcttctgcc agttgatggg ccagcgcttt tacggcgtca
    60541 aattctgccg ggctgatatg aactaattgt tcagtttcgg cgttagtgag cggatctttt
    60601 ggttttttgt cgtttttagc tttcccaaga taatctgcaa tttttgccgc ccattcgatg
    60661 gcttttttct cttcgattcc tttctcaatc aggatagttg ccgcctcacg cgcaattcgc
    60721 ccactgcgaa taccaatatg gcccgccagt gcctgttcaa aaagtgcaga agtgcgccac
    60781 gcacgtttca gactttgtga ggaaacgcgc agacgcgttg caccacccag taccacagtt
    60841 tttggcgcac cagtatcatc acggttaagg ttggcagcag ggtaagcggt taacaaatga
    60901 agctgaataa atgttgtcat gagatagtcc tttattgatt ttgttcgtta tcggcgtcgc
    60961 cagcctggta atattccagc gcccagcgga tacggataaa ttcggttggc cgctgctggc
    61021 ggcggtgatg attgagcagg tcgtcgctct cctgacacca gcggaaaata tcgtctgcca
    61081 aagagataag attgacggaa ccgtttaata atttgactgc gcgtcgtaac tggcgcagta
    61141 attcatccgg cgtttttact gccgacaggc aagtaaaacg cccctctgac attaccgccg
    61201 ccagctgtgc ggcaaaaggc tgccgttcat caatggcttt aacattggcg gcaacggcgg
    61261 cgaccagtgc cagtgcggta aaacgccact cttgtgcttc aattttccac aaagtgtggg
    61321 tctgcatcag cagagaacga aacccttctg acaagcaaac atcattaacc gttgtgctac
    61381 gccgcagact ggcacgattg ccgcgtttag cctgcaactc ttcgtgccat ttacgcagcg
    61441 tggctttgtg cccgtctttt acaatactca ttcagcagcc tcctgctttt tctccctggc
    61501 ggctttagca ctttgcttct ccgccgatgt tgtaaaatat tttttgcgcg cggtcatgac
    61561 gcgttttaaa tcaacgggct cataaggatt ggtgaataca cgctcgtcaa aatcctgacg
    61621 cgagaataac caaatctcct tattccattt gctgagtaat tcatccgcat cctgaccttc
    61681 ttcaatttgg cgcactaacc tcaggaagcg atgctgagtt ttgttccaga agtcgatatc
    61741 cacaaaactg aaatcacccc gtgcaccttt tggatcggag aaccatgctt ctttcaatgc
    61801 actccgtaac agacttagaa tgcgtgaagc cgtttgcgca gccagccgca gtttcggtat
    61861 ctggccttct tttttattga gcagcagcgg gaaacggtgt tcgtaccaac agcgggcttt
    61921 catgttgtcg aaatcatagc caaatcccca taggcctact tttgcctgct tgagactgct
    61981 ggcattaaag agtttcacca ccagagcggg aagttccgta ttgttttctg acttacccgt
    62041 ttcgataagg cctaaccagt cgcgccaaat taaaccgcct ggttgtggtt taactgagta
    62101 aaactcgcca ccctctttaa gtggtacacg gtaaggcgtt aatggatgct gccacatggc
    62161 ataattcgca ccgtaatttt tggtcgtcat caggctcaga agcgcgtcgt cctgctcacc
    62221 gcaaatatcg cagttgccga ctgtcgtggt attaaaatca atacgaatac gccgcggcat
    62281 tccccagtac gcctggagtt tattgacctg atcatcggtt accaccgcac cggccagttc
    62341 gctggtacgc gtcgggccaa gccaggggaa aaccagatcg tcaaattttt tgggtagcgg
    62401 taagtcggct tcatcctgcg gcatcacgtt gagccacagt ttgcgccaca agggggcttg
    62461 ttgattgccc tgatactcct gcaattcaat cagagtcgtc atcggcccac cgccgcgtaa
    62521 accggtgcga tagcctttgc cacctgacgg cgcatttaac tgtagggaga acagagctaa
    62581 cgcagaacaa tgagagcata cgtgttcagt cacgccacgc ttaataaagt ggtctttatt
    62641 aaacttcgtt gtttgagcgc cgggaatctc aggcagtagc gaagcgacct gaactttatc
    62701 gcccatgagc acctcgaaat cctgcataaa tgaaggtgaa tctgggccaa actggaaagc
    62761 gtgttctaat gacagcaatg cttcccgtag cttttcagct tccagcccgt cttcccagat
    62821 atcatcccaa cgacgataat cttttggcgc gaaactgctt tgtagtaacc ccagcaaaaa
    62881 ctgccatgcc gccccctgga gatctgcccg cggcgcagcg atatcgacaa cattttcatc
    62941 cgccagatcg actggcgcca gcttgcctgt tgttccgtct ttaaaacgaa cgggcaacca
    63001 cggggttgtc agaagtgaaa acgagttcat atgtatcctc tcgtaaaaaa gaccatccct
    63061 ggtcggttgt ccatcattcc atccttttcg gtgtatcaac tattcagaga gtcctgtcgc
    63121 aggtatctca atcaaccttg ccaatcaatc cctccctggc cgaatacccg caactttcgt
    63181 catcagtgac taaaatcacg tttgccattt ccggatcttg ccgctgttca atgcaccact
    63241 gcctgaacgc ttccccttcc agtaaagaaa actcatcccg atgttttttc caccagcttc
    63301 gacgcactct gacaacgctc atttcccatg cgtgagcacc ggtggcataa ggcttcacca
    63361 caccggcaat acaggtagcc agccacaggg aaacagattc ctcagccaga cgtgtcgaca
    63421 gcttttccgg aaggtaatcg ttgatattgg cggcatagcc aggcttgaag ttcaggacaa
    63481 actttttagc cattgcgcga tcgcagtaat atttgcccac ttgctcctgc tcgctgcggg
    63541 caaatccttc cggcattacc acgtcctcac cgtagactga ttcaataaga aggcgggctg
    63601 cgtgtggcat ttgaatagcg ccttgctcac gcagtacacg ctgcgtcagc cagattcgtc
    63661 catgatcggg atagacatat gcactgttac gcatggcact gccgaaccat tcgtcaccag
    63721 gagcgtcgtc ccagacggga gccagaatca gcaattcagg aggggaacgc tcgtcttttc
    63781 cgtcacgctt taactgacca ttaatatcgc ggatatgccg ctgtaatcgc cccgctcgct
    63841 gaatcagcaa atcaacaggg gccaggtcgg agatcatttc gtccaggtca caatcaacgc
    63901 tctgctctaa gacctgagta caaatgagga cttttccggc acgcagtgaa ccgtcttctt
    63961 taccaaagcg tgccagcgtc tccatttcaa ttcgctggcg atcgctaaaa gcaaagcggc
    64021 tatgaaagag tgaaaggctg gaagcgggaa tgacgccgcg ggcaagcagc tgacgatgaa
    64081 ccttaatagc gtcatcgaca gaattccgga tccaggcgat gcattttccc tgacttaccg
    64141 ccgattcgat acgcgcaata ctctcttgtt cactatgaag ccaacccacg ctgacgctac
    64201 gctcaacgtc tttgcgcgtc gctacccggt gtgagttcac atcggatttc gtgacatgcg
    64261 tcagccaggg gtaatcatcc ttttcaagga acggagcttc ttgctggccc tctgtgccac
    64321 gcgcaaaggc ggcgacgagt ttgtcgcgct gctgttggga taacgtagca gaaagcaaaa
    64381 tgacgctgtt tccgccacgc gcctgccgct cgatcagccc ttcaagaatg cacgacatgt
    64441 aagcatcaca ggcatggatc tcatcagcca gcaggatttt gttactcaac cccagaagcc
    64501 gcagattatt atgtttaaac ggcatcactg ccatcatcgc ctgatccagc gtgccgacgc
    64561 caatttcagc cagtagcgcc ttcttgttac tgttggcaaa ccaggccgca catccctgac
    64621 tgaatgtttg ttcatccggt tcttctgacc cgactaaatc accggaccag agtgattcat
    64681 tgaagcggtc cattaatgtg cgggcactgt gtgccagcac caggctgggg cgggactctg
    64741 gcgaatagaa agcaagccag gttttgacca gccgatcgta catggcattg gccgttgcca
    64801 ttgttggcag gccaaaaaac aaaccctgtg ctttcctcgc agccatcaac ctgtgcgcca
    64861 ggataagcgc cgcttctgtt ttacctgcgc cagtcacgtc ttccagaata aataactgtg
    64921 gccctggctg gctgatatcc agatccagca ccttttgctg taatggtgtc gggtgctcaa
    64981 taaaaggaaa cagcgtatta actccggtga aaggtgcggt ttctgctttt ggaggaaaga
    65041 cggttaaggc gttttgagcc tgaactaaag ttttctgcca gtaatcttta atatccattg
    65101 ggtgtgcgac gcgtggaaaa aatcgcgttg acgaacccgt ccagtctgcg agtacgactg
    65161 ttgcagagat ataccaggaa agttgtttta aaagttcaac tccctcgtca ttatcccaga
    65221 atgtgggaat ctctatgagc ggacacagtg ccttgatttc aaggagaaaa tctcgcgcgg
    65281 caactttgtc ttcaggcaga aaattatcca gttcatcaat acggtcaggg ggccgaccat
    65341 gatgcccggt agtcatgggc atccacatcg ctattacacg tttaagtttg cgagtagaga
    65401 gtgaagaggc aggaagcaat tcttcgcatt cacttaaata atgatgccac aaccagtagc
    65461 ccagcgttga atgagagatc ttttcgtaat tctttcttgc gccttccgga accttgagtt
    65521 gtgggtgcag gtaaagttgc tgaaatgagc gggcaaattt tccaatatcg tgccagcaca
    65581 acaaccaggc gaaaaattga gccgcctgtt ccttgtcaga aatccctaat tgacgaaaat
    65641 aatcagccag cccgaagcaa tttcttttaa ccattaaata gcccatcgcg gccacatcca
    65701 gcgaatgcca gcaaagaagg tgatagtcgt cacccccttc tttctcgtcg cgtcgggttt
    65761 ttccccagaa atcaaagaaa gtcacaatat ttttatcctt cagtaaactt aaaggatatt
    65821 tacgcatatt ttaaaaatta tctgtgatat atatcagcca ataaaacatt tatattgtga
    65881 ataaatttat gattttcatt tgaaattcat aatgataaat agagactata tattcacata
    65941 aaaaatataa atttaaaaaa tggtgcagga gaccaaacca tcacaattca atacgcgggg
    66001 ttt
//
LOCUS       DAZUCE010000004        65429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZUCE010000004 DAZUCE010000000
VERSION     DAZUCE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.004"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(134..613)
                     /gene="folK"
                     /locus_tag="ACHY03_000219"
     CDS             complement(134..613)
                     /gene="folK"
                     /locus_tag="ACHY03_000219"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFS3903506.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVPQPAIW"
     gene            complement(610..2007)
                     /gene="pcnB"
                     /locus_tag="ACHY03_000220"
     CDS             complement(610..2007)
                     /gene="pcnB"
                     /locus_tag="ACHY03_000220"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFS3903507.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2067..2963)
                     /gene="gluQRS"
                     /locus_tag="ACHY03_000221"
     CDS             complement(2067..2963)
                     /gene="gluQRS"
                     /locus_tag="ACHY03_000221"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFS3903508.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAHARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLLEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTQFTDLLRGSIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(3030..3485)
                     /gene="dksA"
                     /locus_tag="ACHY03_000222"
     CDS             complement(3030..3485)
                     /gene="dksA"
                     /locus_tag="ACHY03_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFS3903509.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3663..4367)
                     /gene="sfsA"
                     /locus_tag="ACHY03_000223"
     CDS             complement(3663..4367)
                     /gene="sfsA"
                     /locus_tag="ACHY03_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFS3903510.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4382..4912)
                     /gene="thpR"
                     /locus_tag="ACHY03_000224"
     CDS             complement(4382..4912)
                     /gene="thpR"
                     /locus_tag="ACHY03_000224"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFS3903511.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGDVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4986..7415
                     /gene="hrpB"
                     /locus_tag="ACHY03_000225"
     CDS             4986..7415
                     /gene="hrpB"
                     /locus_tag="ACHY03_000225"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFS3903512.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGVFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7610..10135
                     /gene="mrcB"
                     /locus_tag="ACHY03_000226"
     CDS             7610..10135
                     /gene="mrcB"
                     /locus_tag="ACHY03_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFS3903513.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDYDDYDDYEEEEP
                     MPRKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLPAA
                     VYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFPD
                     SKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGF
                     PDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLS
                     SERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGR
                     PVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLS
                     ARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKA
                     AVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGSL
                     AKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDAL
                     TRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQT
                     IASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGRQ
                     LGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSIY
                     QRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQQ
                     QPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10355..12598
                     /gene="fhuA"
                     /locus_tag="ACHY03_000227"
     CDS             10355..12598
                     /gene="fhuA"
                     /locus_tag="ACHY03_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFS3903514.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAATTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12649..13446
                     /gene="fhuC"
                     /locus_tag="ACHY03_000228"
     CDS             12649..13446
                     /gene="fhuC"
                     /locus_tag="ACHY03_000228"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFS3903515.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13446..14336
                     /gene="fhuD"
                     /locus_tag="ACHY03_000229"
     CDS             13446..14336
                     /gene="fhuD"
                     /locus_tag="ACHY03_000229"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFS3903516.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14333..16315
                     /gene="fhuB"
                     /locus_tag="ACHY03_000230"
     CDS             14333..16315
                     /gene="fhuB"
                     /locus_tag="ACHY03_000230"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFS3903517.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLIIAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16473..17753)
                     /gene="hemL"
                     /locus_tag="ACHY03_000231"
     CDS             complement(16473..17753)
                     /gene="hemL"
                     /locus_tag="ACHY03_000231"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFS3903518.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17919..17966
                     /gene="yadX"
                     /locus_tag="ACHY03_000232"
     CDS             17919..17966
                     /gene="yadX"
                     /locus_tag="ACHY03_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFS3903519.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17978..19399
                     /gene="clcA"
                     /locus_tag="ACHY03_000233"
     CDS             17978..19399
                     /gene="clcA"
                     /locus_tag="ACHY03_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFS3903520.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19423..19488
                     /gene="yadW"
                     /locus_tag="ACHY03_000234"
     CDS             19423..19488
                     /gene="yadW"
                     /locus_tag="ACHY03_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFS3903521.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19481..19825
                     /gene="erpA"
                     /locus_tag="ACHY03_000235"
     CDS             19481..19825
                     /gene="erpA"
                     /locus_tag="ACHY03_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFS3903522.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19872..20495)
                     /gene="yadS"
                     /locus_tag="ACHY03_000236"
     CDS             complement(19872..20495)
                     /gene="yadS"
                     /locus_tag="ACHY03_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFS3903523.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20533..21333)
                     /gene="btuF"
                     /locus_tag="ACHY03_000237"
     CDS             complement(20533..21333)
                     /gene="btuF"
                     /locus_tag="ACHY03_000237"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFS3903524.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21326..22024)
                     /gene="mtnN"
                     /locus_tag="ACHY03_000238"
     CDS             complement(21326..22024)
                     /gene="mtnN"
                     /locus_tag="ACHY03_000238"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFS3903525.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAIRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22108..23625
                     /gene="dgt"
                     /locus_tag="ACHY03_000239"
     CDS             22108..23625
                     /gene="dgt"
                     /locus_tag="ACHY03_000239"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFS3903526.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23755..25179
                     /gene="degP"
                     /locus_tag="ACHY03_000240"
     CDS             23755..25179
                     /gene="degP"
                     /locus_tag="ACHY03_000240"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFS3903527.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25334..26491
                     /gene="cdaR"
                     /locus_tag="ACHY03_000241"
     CDS             25334..26491
                     /gene="cdaR"
                     /locus_tag="ACHY03_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFS3903528.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26580..26966)
                     /gene="yaeH"
                     /locus_tag="ACHY03_000242"
     CDS             complement(26580..26966)
                     /gene="yaeH"
                     /locus_tag="ACHY03_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFS3903529.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27280..28104)
                     /gene="dapD"
                     /locus_tag="ACHY03_000243"
     CDS             complement(27280..28104)
                     /gene="dapD"
                     /locus_tag="ACHY03_000243"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFS3903530.1"
                     /translation="MQQLQNIIETAFERRAEITPASADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28135..30807)
                     /gene="glnD"
                     /locus_tag="ACHY03_000244"
     CDS             complement(28135..30807)
                     /gene="glnD"
                     /locus_tag="ACHY03_000244"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFS3903531.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEKTRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRYHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30869..31663)
                     /gene="map"
                     /locus_tag="ACHY03_000245"
     CDS             complement(30869..31663)
                     /gene="map"
                     /locus_tag="ACHY03_000245"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFS3903532.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32031..32756
                     /gene="rpsB"
                     /locus_tag="ACHY03_000246"
     CDS             32031..32756
                     /gene="rpsB"
                     /locus_tag="ACHY03_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFS3903533.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32891..33742
                     /gene="tsf"
                     /locus_tag="ACHY03_000247"
     CDS             32891..33742
                     /gene="tsf"
                     /locus_tag="ACHY03_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFS3903534.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33889..34614
                     /gene="pyrH"
                     /locus_tag="ACHY03_000248"
     CDS             33889..34614
                     /gene="pyrH"
                     /locus_tag="ACHY03_000248"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFS3903535.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34764..35321
                     /gene="frr"
                     /locus_tag="ACHY03_000249"
     CDS             34764..35321
                     /gene="frr"
                     /locus_tag="ACHY03_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFS3903536.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35413..36609
                     /gene="ispC"
                     /locus_tag="ACHY03_000250"
     CDS             35413..36609
                     /gene="ispC"
                     /locus_tag="ACHY03_000250"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFS3903537.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36798..37556
                     /gene="ispU"
                     /locus_tag="ACHY03_000251"
                     /gene_synonym="uppS"
     CDS             36798..37556
                     /gene="ispU"
                     /locus_tag="ACHY03_000251"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFS3903538.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37569..38426
                     /gene="cdsA"
                     /locus_tag="ACHY03_000252"
     CDS             37569..38426
                     /gene="cdsA"
                     /locus_tag="ACHY03_000252"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS3903539.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38438..39790
                     /gene="rseP"
                     /locus_tag="ACHY03_000253"
     CDS             38438..39790
                     /gene="rseP"
                     /locus_tag="ACHY03_000253"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFS3903540.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQQRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39820..42252
                     /gene="bamA"
                     /locus_tag="ACHY03_000254"
     CDS             39820..42252
                     /gene="bamA"
                     /locus_tag="ACHY03_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFS3903541.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42374..42859
                     /gene="skp"
                     /locus_tag="ACHY03_000255"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42374..42859
                     /gene="skp"
                     /locus_tag="ACHY03_000255"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFS3903542.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42863..43888
                     /gene="lpxD"
                     /locus_tag="ACHY03_000256"
     CDS             42863..43888
                     /gene="lpxD"
                     /locus_tag="ACHY03_000256"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFS3903543.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIDSGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43993..44448
                     /gene="fabZ"
                     /locus_tag="ACHY03_000257"
     CDS             43993..44448
                     /gene="fabZ"
                     /locus_tag="ACHY03_000257"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFS3903544.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44452..45240
                     /gene="lpxA"
                     /locus_tag="ACHY03_000258"
     CDS             44452..45240
                     /gene="lpxA"
                     /locus_tag="ACHY03_000258"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFS3903545.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45240..46388
                     /gene="lpxB"
                     /locus_tag="ACHY03_000259"
     CDS             45240..46388
                     /gene="lpxB"
                     /locus_tag="ACHY03_000259"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFS3903546.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDTHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTEYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46385..46981
                     /gene="rnhB"
                     /locus_tag="ACHY03_000260"
     CDS             46385..46981
                     /gene="rnhB"
                     /locus_tag="ACHY03_000260"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFS3903547.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47018..50500
                     /gene="dnaE"
                     /locus_tag="ACHY03_000261"
     CDS             47018..50500
                     /gene="dnaE"
                     /locus_tag="ACHY03_000261"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFS3903548.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRSEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50513..51472
                     /gene="accA"
                     /locus_tag="ACHY03_000262"
     CDS             50513..51472
                     /gene="accA"
                     /locus_tag="ACHY03_000262"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFS3903549.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51571..53712
                     /gene="ldcC"
                     /locus_tag="ACHY03_000263"
     CDS             51571..53712
                     /gene="ldcC"
                     /locus_tag="ACHY03_000263"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFS3903550.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEEWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53769..54158
                     /gene="yaeR"
                     /locus_tag="ACHY03_000264"
     CDS             53769..54158
                     /gene="yaeR"
                     /locus_tag="ACHY03_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS3903551.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54223..55518
                     /gene="tilS"
                     /locus_tag="ACHY03_000265"
     CDS             54223..55518
                     /gene="tilS"
                     /locus_tag="ACHY03_000265"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFS3903552.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQVFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLLWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADDLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GLGSVQLNAGGAIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGISFVWQRNA"
     gene            complement(55571..55831)
                     /gene="rof"
                     /locus_tag="ACHY03_000266"
     CDS             complement(55571..55831)
                     /gene="rof"
                     /locus_tag="ACHY03_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFS3903553.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55818..56018)
                     /gene="yaeP"
                     /locus_tag="ACHY03_000267"
     CDS             complement(55818..56018)
                     /gene="yaeP"
                     /locus_tag="ACHY03_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFS3903554.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56184..56729
                     /gene="yaeQ"
                     /locus_tag="ACHY03_000268"
     CDS             56184..56729
                     /gene="yaeQ"
                     /locus_tag="ACHY03_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFS3903555.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56726..57148
                     /gene="arfB"
                     /locus_tag="ACHY03_000269"
     CDS             56726..57148
                     /gene="arfB"
                     /locus_tag="ACHY03_000269"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFS3903556.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKEHLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKELTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57162..57872
                     /gene="nlpE"
                     /locus_tag="ACHY03_000270"
                     /gene_synonym="cutF"
     CDS             57162..57872
                     /gene="nlpE"
                     /locus_tag="ACHY03_000270"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFS3903557.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEAAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58072..58896)
                     /gene="yaeF"
                     /locus_tag="ACHY03_000271"
     CDS             complement(58072..58896)
                     /gene="yaeF"
                     /locus_tag="ACHY03_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFS3903558.1"
                     /translation="MDKPKAYYRLLLPCFLLLSACTVDISQPDSSATAVDVEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58949..60667)
                     /gene="proS"
                     /locus_tag="ACHY03_000272"
     CDS             complement(58949..60667)
                     /gene="proS"
                     /locus_tag="ACHY03_000272"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFS3903559.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60778..61485)
                     /gene="tsaA"
                     /locus_tag="ACHY03_000273"
     CDS             complement(60778..61485)
                     /gene="tsaA"
                     /locus_tag="ACHY03_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFS3903560.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61482..61886)
                     /gene="rcsF"
                     /locus_tag="ACHY03_000274"
     CDS             complement(61482..61886)
                     /gene="rcsF"
                     /locus_tag="ACHY03_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFS3903561.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62004..62819)
                     /gene="metQ"
                     /locus_tag="ACHY03_000275"
     CDS             complement(62004..62819)
                     /gene="metQ"
                     /locus_tag="ACHY03_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFS3903562.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKNGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62859..63512)
                     /gene="metI"
                     /locus_tag="ACHY03_000276"
     CDS             complement(62859..63512)
                     /gene="metI"
                     /locus_tag="ACHY03_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFS3903563.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63505..64536)
                     /gene="metN"
                     /locus_tag="ACHY03_000277"
     CDS             complement(63505..64536)
                     /gene="metN"
                     /locus_tag="ACHY03_000277"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFS3903564.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64724..65299
                     /gene="gmhB"
                     /locus_tag="ACHY03_000278"
     CDS             64724..65299
                     /gene="gmhB"
                     /locus_tag="ACHY03_000278"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFS3903565.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15380 a        16306 c        18080 g        15663 t
ORIGIN      
        1 aatgtaaatt aagtatattt aatgtgtaag gggggatgtg aggagtaaga taagaaaata
       61 attttttctt ggtggatgaa atagccgtat cattttgtat tcctctcgtt aagaaaataa
      121 aataatacag ctattaccag atggcaggtt gtggtactgc tagtgaagtg agtatttcac
      181 gtaatgtttc cccatcagga aacaccaact ccggcgcgat ttcaaacagc ggccacagca
      241 taaatccacg atttttcata tcgtagtgcg gtacggtcag tcgttcagta tttatcactt
      301 cattaccaaa cagcatgatg tcgagatcca gcgtgcgcgg tccccagcgt tcagctttac
      361 ggacgcgacc ttgctgcaat tcaatacgct gtgtgtgatt gagtagctct tcaggtgcaa
      421 gagaggtttc cagcgccacg gctgcgttta agtaatcggg ttgatcttgc ggccccagcg
      481 gtggggtgcg gtaaaacgaa gaaacggtaa gaatgtggct ttcagggata tcgcctaatg
      541 ctttcagggc agcattgacc tgctccagcg gagaggccag attgctgcct atggcaatat
      601 acgccactgt catgcggtac cctcacgacg tggtgcgcgt ttgcgtggac gacgagtgcg
      661 acgacgcggt gacggttctt catccagctc gttgagcatc cctttttggt ctggtggcgc
      721 ggaaacctgg aactcacccc accatttcac cagacgctgc agttcagcgt tacgctcaac
      781 ttcagctcgc aaggccaaca ggtcataagc cgcacggaac ttaggatgct ccagcagttt
      841 ccatgcacgt ttaccctgac gtcgggacat acgcaactgc aactgccaga tatcgcgggt
      901 taacgttgtc agacgtttcg ggattgccag tgaacggcag gcttcgtcca gcacgtcgtt
      961 catcgccagc gcgaaagcgt cgtgataggt caggccgctt tcctgggcga tcttctgtgc
     1021 cgtctccagc agtgggtacc agaacatggc ggcaaacagg aacgccgggt tcacgcgcat
     1081 atcgttatgg atacgcgtat cggtattctt cagcacctgt tcaatgatcc gctccatcgg
     1141 gctgtcgcca ttttccgtga agtagcgggt aatggtcggg aacagcggct ggaacagatg
     1201 atattcacac agcagcttat aggtttcgta accgtagccc gcttgtagca gtttaagcga
     1261 ttcttcaaac aggcgtgccg gtgggatatc gttcagcagg gtagcgaggc gagggatcgg
     1321 ttctgcggtt tccgggctga tgcgcatacc caatttggcg gcaaaacgta ccgcgcgcag
     1381 catacgtacc ggatcttcac ggtagcgcgt ttccgggtta ccaatcagac ggataacgcc
     1441 gtccttcaga tccttcatac cgccaacgta atcacggacg gtaaaatccg ctacgctgta
     1501 atacaggctg ttgatagtga aatcgcggcg ctgggcgtct tcttcgatgg agccgaaaat
     1561 gttgtcgcgc agcaacatgc cgttttgccc gcgttgggag gtcgtgcggt cgctgacgtt
     1621 accttcgtgg tgtccacgga aggttgcaac ttcgataatc tccgggccaa acatcacatg
     1681 agccagacgg aaacggcgac ctaccaggcg gcagttacgg aacagtttgc gcacctgctc
     1741 aggcgtggcg ttagtggtta cgtcaaaatc tttcggcttt ttgccgagta acaggtcgcg
     1801 cacgccgccg ccaaccagcc aggcttcgta tcccgcttta ttgagcctgt acattacctt
     1861 cagggcattt tcactgatat ctttgcggga aatagcatgc tgctcacgcg ggatcaccgt
     1921 cacctgtgga cgggcgactg cctgttcagc ctcgctttcc tcgcggctta gcaccttgcg
     1981 gcaaaaatta gcgactcggg taaaaatagt acacctcggt agtgtcaaac atcattcagg
     2041 acaaaaaaat agcggctaat catagctcag catgacgcat ttgagaatgt tgaatttaca
     2101 attgccgact caggcactgc ggtaagccgc cagtttttga cggctgactg aaggatttgc
     2161 tcgacgctga aatcctgcca gtgtgcttct gcctgctgcc ccagaaattg aagtgccgcg
     2221 attagtaccg ggcgtggatc gcctttcggc aacgcaggcg catgattctg cttggaaagt
     2281 ttagcgcctt gtggattaag cgccagcggc agatgaatgt aatctggcac tttccagcca
     2341 aaaagctggt acagcgagat ttgccgcact gtcggttcga tcaaatctgc cccgcggaca
     2401 atttcgctca cgccctggaa gtgatcatcg accaccaccg ccaggttata ggcaaacaaa
     2461 ccatcacggc gatgaataat aaaatcttcc cgtgccagtt tttcgtcggc gtgaatgctg
     2521 cctcgcagca ggtcagtaaa ctgggtgacc ggatgctgct ggcggatacg cactgcggcg
     2581 ttgtctggtc catgatgcaa ttcccggcaa tgaccgtcgt aaataccgcc aatgctttga
     2641 atacgtgcac gcgtgcaggt gcagtaataa cttagtcctt gctcaagtaa ccaggcgagt
     2701 gcttcgcgat aggcgtggtg acgttgcgat tgccagagaa catcgccgtc ccagtgcagg
     2761 ccgtaatgtt ccagctggcg caggatagtt tctgcggcac cgggaacttc acgaggcggg
     2821 tcgatatctt ctatgcgtac cagccagcga ccttgccggg cgtgagcctg caaatagctg
     2881 ccaagcgcag caatcagaga accaaaatga agctcgccgg aaggagaggg ggcgaagcgg
     2941 ccaatatagt gtgtgtctgt catctcttcg aacaaaaaat aaggcgggag catttcccgc
     3001 ctgtggtaaa cgtgatggaa cggctgtaat tagccagcca tctgtttttc gcgaatttca
     3061 gccagcgttt tgcagtcgat gcacagatcg gctgtcgggc gcgcttccag acggcgaata
     3121 ccaatttcaa caccgcagga ttcacagtag ccgaaatctt cgtcttccac ttttttcagc
     3181 gtcttctcga tctttttgat cagcttacgc tcgcgatcgc ggttacgcag ttcgaggctg
     3241 aactcttctt cctgggctgc acggtctacc gggtccggga agttggctgc ttcatcctgc
     3301 atatgtgtaa cggtgcgatc gacttcatcc ctgagttgat tacgccatgc ttccagaata
     3361 cgacggaagt gcgccagctg ggcttcattc atatactctt cgcccggctt ctcctgatat
     3421 ggttccaccc cagcgatggc gagaatactc agggacgatg ttttacggtt ttgcccttct
     3481 tgcatgttgc ttctccttaa cacgcactat cgatccccat gttcggggga aaaatgaggc
     3541 cgctataaat agcagatgct tttccggata gcaattatct aaacgtaaca cttgacaact
     3601 gtgtgaggaa aagcgtattt gcgcacgcga ccagaatgta aattaaccag ttacttactt
     3661 tactacaatg taaccggcag tgatttttta agagccatgc cttcagcaga aatttccgct
     3721 ttgtaagcca gaatttctac ccccctctgt tgagcttctg acaatagttg cgcgtatttc
     3781 tcatcgatgt ggcgcgcggg tgaaaaccgt gtaatggctg aatgcagcac ggcgaaaaag
     3841 ataaccgcac gctggccttc agccgctacg ctcatcaact cccgaaggtg tttctgacct
     3901 cgttcagtga ccgcatcggg aaaatatccc tgttcgttct ccgctaacgt aaccgatttc
     3961 acttcaatat agcagtctgg acgcgaatcc gcctgcaaca taaagtcaat acggctgcgt
     4021 tctgcgccgt attttacttc gcttttcagc gagctatagc ctgacagttc tgaaattgat
     4081 tcattaagga tagcctcttt cgtcaacctg ttagcccaaa gcgtgttgac gcaaataaat
     4141 gcgccgctct ggctttgagt taattcccag gtgtgtgggt atttccgttt ggtgttgtct
     4201 gaagtcgaat accagacggt atcgccaggc gttgcacaac cggtcatcgc acccgtattc
     4261 gggcagtgta gcgttaattc gcgaccatcg ggtgtgatca catcggctaa aaaacgtttg
     4321 taacgctgaa ttagcgtcgc gcgctgtaga gggggagaaa attccattgc gacaatcctt
     4381 gttattgcgt tagcgcccag cgttttagcg gcgtgtagcg tgtgcgtcca cgggcaaacg
     4441 aggaggcgta aagggtgaac tccgtcaccg catacgacca gttaaaacct ggcggcggga
     4501 ttgtcaccgc ctcgctggcg tcgcgcaata aggtaatatg tggatgaaac ggacgattgc
     4561 tttgaaaaca accgctgcgg gcagcctgtg aacggagcat attcgccagc tggattaagc
     4621 cgcgtggagg ctgacgcatc cctaaccaca ccacacgcga acgcagccat tgtccggcgt
     4681 catcaagcgt gagtgtgaaa ccaggttgac gaatccgtcc ggctaaaaga gaaagcgcct
     4741 tctctttctc ggcgctcacg tcgcctaaaa atgccagagt cagatgcaaa ttatcggcgg
     4801 cgaccggacg tcccgcctca agtgggaagt gtgtggcgcg ccagtggata atctgttcgc
     4861 ggatttctgc aggtaagtcg atagcaaaga acagacgttg cggttcagac atgtgaggca
     4921 ctcggttatg aattaccgcg atgctacaat gtggcgcgaa gaatgttaac cctctggagc
     4981 gttttgtgtc gtcgttgccc gttgctgccg tcttacctga attacttacc gctctcgatt
     5041 gtgcgccgca ggtgttatta agtgcgccga ccggggccgg gaaatcaacc tggctgccgc
     5101 tgcaactgct ggcgcatccc ggcattaacg ggaaaattat cctgctggag ccgcgtcgtc
     5161 tggcggcgcg taacgtcgcg caacggctgg cggaactgct taacgaaaag ccaggcgata
     5221 ccgttggcta ccggatgcgt gcgcaaaact gcgtcgggcc gaatacccgc ctggaagtgg
     5281 ttaccgaagg cgtgctgacg cggatgatcc agcgtgaccc ggaactgagc ggtgttggac
     5341 tggtgatcct tgatgaattt catgagcgca gcttgcaggc ggatttggcg ttggcgctgt
     5401 tactcgatgt gcaacaaggt ctgcgtgatg accttaaact gctgattatg tcggctacgc
     5461 tggataacga ccgcttgcag caaatgctgc cagaagcgcc cgtcgtcatc tcagaagggc
     5521 gctcgtttcc ggttgaacgc cgttatttac cgctgcccgc gcatcagcgt tttgacgaag
     5581 ccgtcgcggt tgccaccgcc gaaatgctgc gtcaggaaag tggatcatta ctgttatttt
     5641 tacctggcgt cggagaaatt cagcgtgtgc aggaacaact ggcttcgcgc atcggcagtg
     5701 atgtgttgct ctgcccgctg tatggcgcgt tgtcgctgaa cgatcagcga aaagcgatcc
     5761 tcccggcacc gcaagggatg cgcaaagtgg tgctggcgac caatattgct gaaaccagtt
     5821 taaccattga aggtattcgt ctggtggtgg attgtgccca ggagcgtgtg gcgcgttttg
     5881 atccgcgcac ggggcttacg cgactgatta ctcaacgcgt tagccaggca tccatgacgc
     5941 agcgtgccgg gcgcgccggg cgtctggagc cgggtatctg cctgcattta atcgccaagg
     6001 aacaagcaga acgcgccgcg gcgcaaagtg aaccggaaat attacaaagc gatctttccg
     6061 gtttgctgat ggaattactg caatggggat gcagcgatcc ggcgcagatg agctggctgg
     6121 atcaaccgcc aacggtgaat ctactggccg cgaaacgcct gttacggatg ctgggggcgc
     6181 tggacggtga acggcttagt gcgcaagggc aaaaaatggc agcgctgggt aacgatccgc
     6241 gtttagcggc aatgctggtt agcgcaaaga gcgacgacga agctgctacc gcggcaaaaa
     6301 ttgccgccat tctcgaagag ccgccacgga tgggcaatag tgacctgggt gtggcgtttt
     6361 cgcgcaatca accagcctgg cagcaacgta gtcagcaact gttaaaacgc ttaaacgtac
     6421 gcggcggtga ggcagacagt tcgcttatcg cgccgctact tgccggggtg tttgccgatc
     6481 gcattgctcg ccgccgtggg caagatggac gctatcaact ggcaaacggc atgggggcga
     6541 tgcttgatgc cgacgacgcg ctaagtcgcc acgaatggtt gatcgcgccg ttattattgc
     6601 agggcagcgc ctcgccggat gcgcggattt tactggcgtt accggtcgat attgatgaat
     6661 tagtacaacg ctgtccgcag ctggtgcagc aatctgacac cgtggaatgg gatgacgcgc
     6721 aaggtacgct gaaagcctgg cgtcggctgc aaatcggtca gttgacggtg aaagtgcagc
     6781 cgttggcgaa accgtcagaa gacgagttgc atcaggcgat gcttaacggc atccgtgata
     6841 aaggtttaag cgtgctcaac tggacggcgg aagcggaaca gctacgcttg cgtttgttat
     6901 gcgccggaaa gtggttgcca gaatatgact ggccagcggt tgatgatgaa agtttgttgg
     6961 cgacgctgga aacgtggctg ctgccacata tggccggtgt acattcacta cgcggcctga
     7021 aatcactcga catttatcag gcactacgcg gattacttga ttggggaatg cagcaacgtc
     7081 tggatagtga attgcctgcg cattacactg tgccgacggg aagccggatc gccattcgtt
     7141 atcatgaaga taacccgccc gcgctggcgg tgagaatgca ggagatgttt ggtgaggcca
     7201 ctaatccgac gatcgcccag gggcgcgtgc cgctggtgct ggaattgctt tcacctgccc
     7261 aaaggccgct gcaaatcacg cgtgatttgg gggccttctg gaaaggggcg tatcgtgagg
     7321 tgcaaaaaga gatgaaaggg cgttatccca aacacgtctg gccggacgac ccggcaaaca
     7381 cggcaccgac gcgacggacg aaaaagtatt cgtaaaacgg gtatgagcag tcccctcgcc
     7441 cctatgggga gagggatagg gtgaggggta atgtgcgcgc tgatgccctc accccggccc
     7501 tctcccacag ggagagggag aaaagcaaat ttgagagata tcttcttctg tcttgcaaca
     7561 gaagaacaga aaatcgggct tttgcgcctg aatattgcgg agaaaaagca tggccgggaa
     7621 tgaccgcgag ccaattggac gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag
     7681 ccgtcgtcgt tacgaagatg acgactacga cgattatgat gactatgagg aagaagaacc
     7741 gatgccgcgc aaaggtaagg gcaaagggcg taagcctcgt ggcaaacgcg gctggctatg
     7801 gctgctgtta aaactggcta tcgtttttgc cgtgctgatc gccatttacg gcgtttatct
     7861 cgatcaaaaa attcgtagtc gtattgatgg caaggtctgg caattgcccg cggcagttta
     7921 tggccgaatg gtcaaccttg agccagacat gaccatcagc aagaacgaga tggtgaagct
     7981 gctggaggca acccagtatc gtcaggtgtc gaaaatgacc cgccctggcg aatttaccgt
     8041 gcaggccaac agcattgaga tgatccgccg tccgtttgat ttcccggaca gtaaagaagg
     8101 gcaggtgcgt gcgcgtctga cctttgatgg cgatcatctg gcgacgatcg tcaatatgga
     8161 gaacaaccgt cagtttggct tcttccgtct tgatccacgc ctgatcacca tgatctcttc
     8221 gccaaacggt gagcaacgtt tgtttgtgcc gcgcagtggt ttcccggatt tgctggtgga
     8281 tactttgctg gcgacagaag accgtcattt ctacgagcat gacggcatca gcctctactc
     8341 catcggacgt gcggtgctgg caaacctgac tgccggacgc acggtgcagg gagcgagtac
     8401 actgacgcaa cagctggtga aaaacctgtt cctctccagc gaacgttctt actggcgtaa
     8461 agcgaatgaa gcctacatgg cgctgatcat ggacgcgcgt tacagcaaag accgtattct
     8521 tgagctgtat atgaacgagg tgtatctcgg tcagagcggc gacaacgaaa tccgcggctt
     8581 cccgctggcg agcttgtatt actttggtcg cccggtagaa gaattgagcc tcgatcagca
     8641 ggcgctgttg gttggtatgg tgaaaggggc gtccatctac aacccgtggc gtaacccgaa
     8701 actggcgctg gagcgacgta atctggtact gcgtctgctg caacagcaac agattattga
     8761 tcaagaactc tatgacatgt tgagtgcgcg tccgctgggg gttcagccgc gcggtggggt
     8821 gatctctcct cagccagcct ttatgcaact ggtgcgtcag gagcttcagg ctaaattagg
     8881 cgataaggta aaagatctct ccggcgtgaa gatcttcact acctttgact cggtggccca
     8941 ggacgcggca gaaaaagccg ccgtggaagg cattccggca cttaagaaac agcgtaagct
     9001 gagcgatctg gaaaccgcga ttgtggtcgt cgaccgcttt agtggtgaag ttcgtgcgat
     9061 ggtcggaggt tctgagccgc agtttgcggg ctacaaccgt gcgatgcagg cgcggcgttc
     9121 gattggttcc cttgcaaaac cagcgactta tctgacggcc ttaagccagc cgaaaatcta
     9181 tcgtctgaat acgtggattg cggatgcgcc aatcgcgctg cgtcagccga atggccaggt
     9241 ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc ggcagagtga tgctggtgga
     9301 tgcgttgacc cgttcgatga acgtgccgac ggtaaatctg gggatggcgc tggggctgcc
     9361 tgcggttacg gagacctgga tcaaactggg tgtaccgaaa gatcagttgc atccggttcc
     9421 ggcaatgctg ctgggggcgt tgaacttaac gccaatcgaa gtggcgcagg cattccagac
     9481 tatcgccagc ggcggtaacc gtgcaccgct ttctgcgctg cgttcggtaa ttgcggaaga
     9541 tggcaaagtg ctgtatcaga gcttcccgca ggcggaacgc gctgttccgg cacaggcggc
     9601 gtatctgaca ctatggacca tgcagcaggt ggtacaacgc ggtactgggc gtcagcttgg
     9661 ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg actaccaaca ataacgtaga
     9721 tacctggttt gcgggcattg acggcagcac ggtgaccatc acctgggtcg gccgtgataa
     9781 caaccagccg accaaactgt atggagccag cggggcaatg tcgatttatc agcgttatct
     9841 ggctaaccag acgccaacgc cgctgaatct tgttccgcca gaagatattg cagatatggg
     9901 cgtggactac gacggcaact ttgtttgcag cggtggcatg cgtgtcttgc cggtctggac
     9961 cagcgatccg caatcgctgt gccagcagag tgagatgcag cagcagccgt caggcaaccc
    10021 gtttgatcag tcttcacagc cgcagcaaca gccgcaacag caacctgctc agcaagagca
    10081 gaaagatagc gacggtgtag ccggttggat taaggatatg tttggtagta attaacatct
    10141 aagcgtgaaa taccggatgg cgagttgcca tccggtaaaa taacatccca cccaaagtat
    10201 taaccctttc ttttcatctg gttgtttatt aacccttcag gaacgctcag attgcgtacc
    10261 gcttgcgaac ccgccagcgt ttcgaatatt atcttatctt tataataatc attctcgttt
    10321 acgttatcat tcactttaca tcagagatat accaatggcg cgttccaaaa ctgctcagcc
    10381 aaaacactca ctgcgtaaaa tcgcagttgt agtagccaca gcggttagcg gcatgtctgt
    10441 ttatgcacag gcagcggttg aaccgaaaga agacactatc accgttaccg ctgcacctgc
    10501 gccgcaagaa agcgcatggg ggcctgctgc aactattgcg gcgcgacagt cagctaccgg
    10561 cactaaaacc gatacgccga ttcaaaaagt gccacagtct atttctgttg tgaccgccga
    10621 agagatggcg ctgcatcagc cgaagtcggt aaaagaagcg cttagctaca cgccgggtgt
    10681 ctctgttggt acgcgtggcg catccaacac ctatgaccac ctgatcattc gcggttttgc
    10741 ggcagaaggc caaagccaga ataactatct gaatggcctg aagttgcagg gcaacttcta
    10801 taacgatgcg gtcattgatc catatatgct ggaacgcgct gaaattatgc gtggcccggt
    10861 ttccgtgctt tacggtaaaa gcagtcctgg cggcctgttg aatatggtca gcaagcgtcc
    10921 gaccaccgaa ccgctgaaag aagttcagtt taaagccggt actgacagcc tgttccagac
    10981 tggttttgac tttagcgatg cgttggatga tgacggtgtt tactcttatc gcctgaccgg
    11041 tcttgcgcgt tctgccaatg cccagcagaa agggtcagaa gagcagcgtt atgctattgc
    11101 accggcgttc acctggcgtc cggatgataa aaccaatttc accttcctct cttacttcca
    11161 gaacgagccg gaaacgggtt attacggctg gttgccgaaa gagggaaccg ttgagccgct
    11221 gccgaacggt aagcgtctgc cgacagactt taacgaaggg gcgaagaaca acacctattc
    11281 tcgtaatgag aagatggtgg gctacagctt cgatcacgaa tttaacgaca cctttactgt
    11341 gcgtcagaac ctgcgctttg ctgaaaacaa aacctcgcaa aacagcgttt atggttacgg
    11401 cgtctgctcc gatccggcga atgcttacag caaacagtgt gcggcattag cgccagcgga
    11461 taaaggccat tatctggcac gtaaatacgt cgttgatgat gagaagctgc aaaacttctc
    11521 cgttgatacc cagttgcaga gcaagtttgc cactggcgat atcgaccaca ccctgctgac
    11581 cggtgtcgac tttatgcgta tgcgtaatga catcaacgcc tggtttggtt acgacgactc
    11641 cgtaccgctg ctcgatctgt acaatccggt gaataccgat ttcgacttca atgccaaaga
    11701 tccggcaaac tccggccctt accgcattct gaataagcag aaacaaacgg gcgtttatgt
    11761 tcaggatcag gcgcagtggg ataaagtgct ggtcaccctg ggcggtcgtt acgactgggc
    11821 agatcaagaa tctcttaacc gcgttgccgc gacgaccgat aaacgcgatg acaaacagtt
    11881 tacctggcgt ggtggtgtta actacctgtt tgataatggc gtaacacctt acttcagcta
    11941 tagcgaatcg tttgaacctt cttcgcaagt tgggaaggat ggtaatattt tcgcaccgtc
    12001 taaaggtaag cagtatgaag tcggcgtgaa atatgtaccg gaagatcgtc cgattgtagt
    12061 tactggtgcc gtgtataatc tcactaaaac caacaacctg atggcggacc ctgagggttc
    12121 cttcttctcg gttgaaggtg gtgagatccg cgcacgtggc gtagaaatcg aagcgaaagc
    12181 ggcgctgtcg gcgagtgtta acgtagtcgg ttcttatact tacaccgatg cggaatacac
    12241 caccgatact acctataaag gcaatacgcc ggcacaggtg ccaaaacaca tggcttcgct
    12301 gtgggctgac tataccttct ttgacggtcc gctttcaggt ctgacgctgg gcaccggtgg
    12361 tcgttatact ggctccagct atggtgatcc ggctaactcc tttaaagtgg gaagttatac
    12421 ggtcgtggat gcgttagtgc gttatgatct ggcgcgagtc ggcatggcgg gctccaacgt
    12481 ggcgctgcat gtcaacaacc tgttcgatcg tgaatacgtc gccagctgct ttaacactta
    12541 tggctgcttc tggggcgcag aacgtcaggt cgttgcaacc gcaaccttcc gtttctaatt
    12601 tctcttttgg ggcacggatt tccgtgcccg tttcacaagt tggctgttat gcaggaatac
    12661 acgaatcatt ccgataccac ttttgcactg cgtaatatct cctttcgtgt gcccgggcgc
    12721 acgcttttgc atccgctgtc gttaaccttt cctgccggga aagtgaccgg tctgattggt
    12781 cacaacggtt ctggtaaatc cactctgctc aaaatgcttg gccgtcatca gccgccgtcg
    12841 gaaggggaga ttcttcttga tgcccagccg ctggaaagct ggagcagcaa agcgtttgcc
    12901 cgcaaagtgg cttatttacc gcagcagctt cctccggcag aagggatgac cgtgcgtgaa
    12961 ctggtggcga ttggtcgtta cccgtggcat ggcgcactag ggcgctttgg tgcggcagat
    13021 cgcgaaaaag ttgaggaagc tatctcgctg gttggcttaa aaccgctggc gcatcggctg
    13081 gtcgatagcc tctctggcgg cgaacgtcag cgggcgtgga tcgccatgct ggtggcgcag
    13141 gatagccgtt gtctgttgct cgacgagcca acctcggcgc tggatatcgc ccaccaggtt
    13201 gatgtgctgg cgctggtgca tcgtttaagt caggaacgtg gtctgacggt cattgccgtg
    13261 ctgcacgata tcaatatggc ggcacgctac tgtgattatc tggtcgccct gcgcggcggt
    13321 gaaatgattg ctcagggaac gcctgcggaa attatgcgcg gcgaaaccct cgaaatgatt
    13381 tatggcatcc cgatgggtat tttgccgcat ccggcgggtg ctgcacctgt gagttttgtt
    13441 tattgatgag cggcttacct cttatttcgc gccgtcgact gttaacggcg atggcgcttt
    13501 ctccgttgtt atggcagatg aataccgccc acgcggcggc tattgatccc aatcgtattg
    13561 tggcgctgga gtggttgccg gtggaattac tgctggcgct cggcatcgtg ccttacggcg
    13621 tggcggatac catcaactat cgcctgtggg tcagcgaacc accattgccg gactcagtga
    13681 ttgacgttgg tttgcgtaca gaacctaacc tcgaactgct gaccgaaatg aaaccgtcgt
    13741 ttatggtctg gtcggcagga tatggccctt caccagaaat gctggctcgt attgcgccgg
    13801 ggcgtggatt taacttcagc gacggcaaac agccgctggc gatggcgcgt aaatcgctga
    13861 cggaaatggc ggatttactt aacctgcaaa gcgcagcaga aacgcattta gcgcaatatg
    13921 aagactttat ccgcagcatg aaaccccgct ttgtgaagcg tggtgcgcgt ccgttattgc
    13981 tgacgacgct tatcgatccg cgccatatgc tggtcttcgg tccaaacagc ttgttccagg
    14041 aaattctcga tgagtacggc atcccaaatg cctggcaagg ggaaaccaac ttctggggca
    14101 gtaccgccgt cagcatcgat cgtctggcag cgtataaaga cgttgatgtg ctctgttttg
    14161 atcacgacaa cagcaaagat atggatgcgc taatggcaac gccgctgtgg caggccatgc
    14221 cgtttgtccg cgccggacgc tttcagcgcg tacccgcagt ctggttttat ggtgcaacgc
    14281 tctcggcaat gcactttgtg cgcgttctgg ataacgccat tggaggtaaa gcgtgagtaa
    14341 acgtattgcg cttttcccgg cattattgct ggcgctgtta gtgattgtcg ctacggcgct
    14401 cacctggatg aacttctcgc aggcgctgcc gcgtagccag tgggcgcagg ctgcctggtc
    14461 gccggatatt gacgtcatcg agcagatgat ttttcactac agcttgttgc cgcgtctggc
    14521 gatttcgctg ctggtgggcg cgggcctggg gctggtgggc gtgctgtttc agcaagtgct
    14581 gcgtaacccg ctggcggaac cgacgacgtt gggcgttgct acaggcgcgc aactggggat
    14641 taccgtcact acgctctggg cgatccctgg tgcgatggcg agccagtttg ctgcgctggc
    14701 aggggcttgt gttgttggct taattgtctt tggcgtcgcg tgggggaaac ggctgtcgcc
    14761 ggtaacgctg attctcgcgg ggctggtagt gagcctttat tgtggagcaa tcaatcaatt
    14821 actggttatc ttccatcatg accagttgca aagcatgttt ctgtggagca ctggaacgct
    14881 gacgcaaacc gactggggcg gtgttgagcg tttatggccg cagttgctgg gcggtgtgat
    14941 gctgacgttg ctgctacttc gtccgttaac cctgatgggg cttgatgatg gcgtggcgcg
    15001 caatctcggg ctggccttgt cgctggcgcg tctggcggcg ctgtcgctgg cgattgtcat
    15061 cagtgcgctg ctggtgaatg cggtagggat tatcggcttt atcgggttgt tcgcgccgct
    15121 gctggcaaaa atgctggggg cgcggcgtct gctgccgcga ctgatgctgg cgtcgttgat
    15181 tggtgcgctg atcctctggc tttccgatca aatcatcctc tggctgactc gcgtgtggat
    15241 ggaagtgtcc accggttcgg tcactgcgtt gatcggtgcg ccgctgctac tgtggctgtt
    15301 gccgcgttta cgcagcatta gcgcgccgga tatgaaggtc aacgatcgtg tcgcggctga
    15361 acgccaacat gtgctggcgt ttgcccttgc tggcggtgtg ctgctgataa ttgctgtggt
    15421 ggtggcgctg tcgtttggtc gtgatgcgca cggctggacg tgggcgagcg gggcgctgct
    15481 cgatgattta atgccctggc gctggccgcg aattatggcg gcgctgtttg cgggcgtcat
    15541 gctggcggtg gcgggctgta ttattcagcg gctgaccgga aacccgatgg caagcccgga
    15601 agtgctgggg attagctccg gcgcggcgtt tggcgtggtg ttaatgctgt ttctggtgcc
    15661 gggtaatgcc tttggctggc tgttacccgc gggcagtatc ggtgccgcgg tgacgctgtt
    15721 gattattatg atcgccgccg ggcgcggtgg attttcccca caccgcatgt tactggcggg
    15781 gatggcgtta agtaccgcat tcaccatgct tttaatgatg ttgcaggcga gtggtgaccc
    15841 gcgaatggcg caagtgctga cctggatctc tggttcgacc tacaacgcca ccgatgcgca
    15901 ggtctggcgc accggaattg tgatggtgat tttgctggcg attaccccgc tgtgccgccg
    15961 ctggctgacc attttaccgc tgggtggtga taccgcgcgg gccgtggggg tggcgttaac
    16021 gccgacgcga attgcgctgc tgctgttagc ggcttgcctg acggcgaccg cgacgatgac
    16081 cattggaccg ttgagttttg ttggtttaat ggcaccgcat atcgcgcgga tgatgggctt
    16141 tcgacggacg atgccgcaca tcgtgatttc ggcgctggtg ggtggtttac tgctggtgtt
    16201 cgctgactgg tgtgggcgga tggtgctgtt tccattccag atcccggcgg ggctgctgtc
    16261 gacctttatc ggcgcgccat attttatcta tttgctgaga aagcagagcc gttaattttt
    16321 ccatataacg gccctgtacg cctggacgga taaagcgttt acgcgacaac ggatgcccga
    16381 tgcgacgctg gcgcgtctta tcgggcctac aaacggcccc gaatcgtagg tcggataagg
    16441 cgttcacgcc gcatccgaca aaccatgctg gatcacaact tcgcaaacac ccgacgtgca
    16501 gcatcgatgg tgttattgat atcttccatg ctgtgcgcca cagacataaa gcccgcttca
    16561 aacgctgacg gtgccaggta aacaccttcg tccagcatca tatggaagaa acgcttaaag
    16621 cgttccacgt cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag
    16681 aaaataccga acatgccgcc aacgtggttt accaccagcg gaattccggc ttcttctgcc
    16741 gcttccagca gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc gtgaacgccc
    16801 ggctgcgcga cttcattcag acaggcgaaa cccgctgcca tcgcaatcgg gttaccggaa
    16861 agcgtacccg cctgatagac cggacccgtc ggggccagcg catccattac atcacgacga
    16921 ccaccgaatg cgcctaccgg cattccaccg ccgatgattt tgccgaggca ggttaaatct
    16981 ggcactacgc cgtaataatc ctgtgcgcca gccagtgcca cgcggaagcc ggtcatcact
    17041 tcatcgatga tcagcaacgc gccaaattcg tcgcacagcg cacgcagacc tggcaggaac
    17101 tctggctgcg gcggaacaca gttcatattg cctgccaccg gctcgacgat aatacaggca
    17161 atctcttgcg ggtattgctc aaacgcggcg cgtacagaag ccagatcgtt ataagtacag
    17221 gttaaggtat gtttggcgaa atctgccgga acacccggcg agtttggctg gcctaacgtg
    17281 agtgcgccag aaccggcttt caccagcagg cagtcagcgt gaccgtggta acaaccttca
    17341 aatttaatga ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatcgtc
    17401 gcttcagtgc cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc
    17461 agttgcgcca ttttcacttc catttcggtc ggtgcaccaa agcttaaacc acgctcggcg
    17521 gcttcaatca cggcattgcg gatagccgga tggttatggc ccagcaccat cggcccccag
    17581 gaaccgacat aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct
    17641 ttttcgataa acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca
    17701 ccgccaggga tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt
    17761 tcctgattcg tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata
    17821 ctcgtcaaac atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt
    17881 ttgtgcattt gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc
    17941 cgtcatctta tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt
    18001 agaaacaccg caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg
    18061 cgataaaacc ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttgtcgggct
    18121 ggctgcggtt gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgtt
    18181 ggtacatact gctgataatt atccgctact gttaaccgtc gcttttctct gttcggcggt
    18241 gctggcgatg ttcggctact ttttggtgcg caaatacgcg cccgaagcag ggggatcggg
    18301 gatcccggaa attgaagggg cgctggaaga tcaacgtcct gttcgctggt ggcgtgtatt
    18361 gccggtgaaa ttctttggcg ggctggggac actcggcgga ggcatggtgt tggggcgcga
    18421 agggccaacc gtgcagatcg gcggtaatat cgggcggatg gtactcgatg ttttccgcct
    18481 gaaaggtgat gaagctcgcc atacgctgtt ggcaaccggt gcggcggcgg gtctggcggc
    18541 ggcctttaac gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt
    18601 tcgctatacg ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat
    18661 gtaccggatt tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgacgcccc
    18721 gctcaatacg ctttggcttt atctgatcct cggtattatt tttggcattt tcggccctat
    18781 ttttaataaa tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat
    18841 taccaaatgg gtgctaatgg gcggtgcgat tggcggtctg tgtggattgc tggggtttgt
    18901 ggcaccagca acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt
    18961 cagcatggga atgctggtgt ttatcttcgt cgcgcgggtc attaccacct tactctgctt
    19021 ctcttccggc gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg
    19081 aaccgctttc ggaatggttg ccgttgagct gtttccgcaa tatcaccttg aggcggggac
    19141 atttgctatt gccggaatgg gggcgttact ggcggcatct attcgcgcgc cgttaacggg
    19201 gatcattctg gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac
    19261 cggtcttggc gcaacactat tagcgcaatt taccggtggg aaaccgctat actcggcgat
    19321 tcttgcgcga acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc
    19381 agccagcgag aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg
    19441 ttagaatttg cccaattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact
    19501 gccgctggag tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga
    19561 taacccgaat ctgaaactac gcgtgtatat caccggtggc ggttgcagcg gcttccagta
    19621 tggtttcacc tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt
    19681 tggcctggtg gttgacccga tgagcctgca atatctggtc ggcggttctg ttgactatac
    19741 cgaaggtctg gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg
    19801 ttgcggttct tcctttagta tctaatctgt tgttcatgtg ccggataaac tatccggcac
    19861 atcttctctc ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa
    19921 cgaatagccg ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc
    19981 agtggtacgg aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca
    20041 gttgcgtaga tttctgtacg taaaatcatg gggatttcgc gggccagaac atcacgaatg
    20101 atcccgccgc caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc
    20161 gcattaaagg ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc
    20221 accggcaaca tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg
    20281 ctggttacca ttgcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga
    20341 tccagcgcca tgtcgcgaat tgtcccgccg cctactgcgg taaccacgcc caataccaga
    20401 acaccaaaag ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc
    20461 gctgtgccga ctatatccag ccaatagacg agcattgttc aatccccact gagcacctaa
    20521 aaaggtcagg cgctaatcta cctgtgaaag cgcattacag agctgttgtg cagcgaggat
    20581 aatacgtggg cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag
    20641 ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat
    20701 cgcctgtggc gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct
    20761 gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttttgaa tcgactcttt
    20821 tccactggta aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc
    20881 agcatattgc gctttcaatt gcgcgtactg atccagcagg gattgcgcgg cttgttcagc
    20941 cttatccggt tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat
    21001 gcttgtcgca tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg
    21061 ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc
    21121 cagattcatc ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc
    21181 ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc
    21241 gggagaaagc gtgatgacgc gcggtgccgc gttgagccac agtggcgcga gaaaagacag
    21301 ggcgaccagc gccctgaaca gtgacttagc catgtgccag tttctgcacc agtgactcaa
    21361 ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact
    21421 gttgatcggc cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc
    21481 agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc
    21541 ggattttcgc cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac
    21601 gtatagcgtt aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt
    21661 cagctttaaa gcctgccgga cagcctggta actgaccgta ttcataacca aatgccgtga
    21721 catccgcgtc gtgataacgt gcttcgtccg agacaacgat atcgcccact ttcaacgttg
    21781 gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca
    21841 gcaaagtggc acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct
    21901 cggttccatt cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacggt
    21961 tttcgatttt gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt
    22021 tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg
    22081 aagcgtattt ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac
    22141 tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc
    22201 ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag
    22261 acccaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg
    22321 gaagtccagc aggtggggcg ctacatcgcc aaagaaattt taagccgtct gaaagagctt
    22381 aaattgctgg aagcatacgg cctggatgaa ctgaccggac cttttgaaag cattgttgag
    22441 atgtcatgcc tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcggcg
    22501 ataaatgact ggtttcgcca gcgtttatac ccggaagatg ccgaaagcca gcctctgact
    22561 gacgatcgct gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag
    22621 ctgcggcgca agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt
    22681 ctggtgcata cattgatgcg gatgaatctc acctgggcgc aggttggcgg tattttaaaa
    22741 tatacccgtc cagcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa
    22801 aagccaggtt attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat
    22861 ttggcgcttt acagtcgttt tccattaacg tggattatgg aagctgccga cgacatctcc
    22921 tattgtgtgg cagatcttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt
    22981 tatcatcatt tgcacgaggc gtggggccag catgagaaag gttcgctctt ttcgctggtg
    23041 gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag
    23101 ttttttatgt atttacgggt aaacacccta aataaactgg taccctacgc ggcacaacga
    23161 tttattgata atctgcctgc aattttcgcc ggaacgttta atcatgcatt attggaagat
    23221 gccagcgaat gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtgttt
    23281 agccatccag atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta
    23341 gagatttatc gccctttatt aagcctgtcg ttatccgact ttactgaact ggtagaaaaa
    23401 gaacgggtga aacgtttccc tattgaatcg cgcttattcc acaaactctc gacgcgccat
    23461 cggctggcct atgtcgaagc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta
    23521 tgggaatatt attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc
    23581 tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa
    23641 gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg cgctataaaa
    23701 cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa
    23761 aaaaccacat tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc
    23821 tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca
    23881 ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc
    23941 gttaatacgc cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc
    24001 tgccaggaag gttctccgtt ccagagctct ccgttctgcc agggtggcca gggcggtaat
    24061 ggtggcggcc agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat
    24121 aaaggctatg tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccag
    24181 ctgagcgatg gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc
    24241 gcgctgattc agatccagaa cccgaaaaac ctgaccgcaa ttaagatggc ggattctgat
    24301 gcactgcgcg tgggtgatta caccgtagcg attggtaacc cgtttggttt gggcgagacg
    24361 gtaacttccg ggattgtctc tgcgctgggg cgtagcggcc tgaatgccga aaactacgaa
    24421 aacttcatcc agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac
    24481 ctgaacggcg aactgatcgg tatcaacacc gcgatcctag caccggacgg cggtaacatc
    24541 ggtatcggtt ttgctatccc gagtaacatg gtgaaaaacc tgacctcgca gatggtggaa
    24601 tacggccagg tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ctccgaactg
    24661 gcgaaagcga tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat
    24721 tcctccgctg caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag
    24781 ccgatcagca gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa
    24841 ctgaccctgg gcttgctgcg cgacggtaag caggtcaacg tgaatctgga actgcagcag
    24901 agcagccaga atcaggttga ttccagctcc atcttcaacg gcattgaagg tgctgagatg
    24961 agcaacaaag gcaaagatca gggcgtggta gtgaacaacg tgaaaacggg cactccggct
    25021 gcgcagatcg gcctgaagaa aggtgatgtg attattggcg cgaaccagca ggcagtgaaa
    25081 aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt
    25141 cagcgcggcg acagcaccat ctacctgtta atgcagtaat ctccctcaac cccttcctga
    25201 aaacgggaag gggttctcct tacaatctgt gaacttcacc acaactccat acatcttcat
    25261 catcctttag gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca
    25321 taacggagga cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc
    25381 acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgcg ggcgaattat
    25441 cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggtgcattgc tggtactttc
    25501 acagggacga gtcgtcgata tcgatgacgc ggtagcacgt catctgcacg gtgtgcggca
    25561 ggggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg
    25621 tgaaccagag aacctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct
    25681 ggaacagtcg cggttgatgc acttgttggc gcaggatagc cgtttgcggg aggaactggt
    25741 gatgaacctg attcaggcag aggagaatac tcccgcactt actgaatggg cgcaacggct
    25801 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25861 tggtgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25921 acgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25981 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    26041 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttcg ctgggcaact attttaccgg
    26101 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26161 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26221 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26281 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgtc acaatgtgca
    26341 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26401 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26461 tgtggcgtta caactggatg aagagcggta ggttatgcgt taaggttgag gcggcgctgg
    26521 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26581 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26641 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26701 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26761 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26821 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26881 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26941 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    27001 tattttagca tttggcctgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    27061 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27121 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27181 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgaaccaga
    27241 aagcaatcgg gcagcgtcgt gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag
    27301 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27361 gtagaggctg tatttgccat ctttcgacgg cagattacct gaaacaacca cagaccccgc
    27421 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27481 aatgtatacg cccatggaaa tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27541 agagcgcgcg ccgatgaagc aattatcttc aatgatggtt gggttagcct gcagcggttc
    27601 cagcacaccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27661 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27721 gacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27781 aggtggtaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27841 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27901 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27961 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    28021 gcgggtaacg gtgtctgcac tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    28081 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28141 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28201 attaagtgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28261 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28321 cgccagcagc ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28381 ggttggcaaa aacgtcactt cggtttcaac agtaaaatgg cgcaatttgg cgggttggcg
    28441 acggggctgc ggtggctgcc agctactttg tgtcagtact tgctccagac caaaccgaat
    28501 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28561 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tggacactta aattgcggcg
    28621 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28681 ctcggtgcct ccacgcgtag cctgcggact aagcaatacc agcggtttgc ttaaatcatg
    28741 ctgtaataaa tggcgcgcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28801 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28861 tagtgccagc gcctggagtt ggtgatagcg aacccgttcg cgcatatccg gcgtgttttg
    28921 cattccgcgt cgtagctgtt tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28981 ccagctattc cacagcgttt cgttggtggc acaaatgtca gccactgtca ggcataccag
    29041 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttccgggtc
    29101 ctgaatatcg cgacgctgag ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29161 gaccagctgt gtttcgcgtg agttcagccc gtggagttcg gcaaaatgca ctacatcctg
    29221 agcaccgaga atagagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29281 gatgaaaatc agctcagttg acggtaggcg cggccagacg tccacacaca acggatggcg
    29341 ctggcgcgtt ttttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29401 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gtgaccattg
    29461 cggcatatac gcgccgagta cgctatggcg atgcattggc aatagcccgc gccgcaccgc
    29521 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tccggaatat tacacagcgg
    29581 ttgttgcaga tggcgccggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29641 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29701 aaatagtgtt tcatcacgca agtcgatcag cgtgccgcgt agctgaaatt catcgtcaat
    29761 cggacggggc ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29821 catctggttg agttcactga cgcggcgcgt aacgcggaag taatccttca tcatccgctc
    29881 gaccggttcg ttaccttcac cactgtaatt cagacgctgg gcgacgctaa gctggcgatc
    29941 gaataacagg cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatccgcca
    30001 caatatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    30061 gaccatttca tccaacgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30121 gatatcgcgc aagccaccag ggctactttt aatgtctggt tcaaggttgt agctggtgcc
    30181 gtggtaacgc tgatggcgct ggttctgttc ttcaactttc gccgcgtaga acttgtcgga
    30241 aggccagaat ccttcgctga aaatatgttt ttgcagttcg aggaacagcg caacatcgcc
    30301 aattaataag cgggattcga tcaaattggt ggcgacggtt aaatccgata acccttccag
    30361 catgcactct tcaagcgtgc gcacgctatg acccacttcc agctttacat cccagagcag
    30421 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30481 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcgac
    30541 caatgccagg tcggcaatct ggctgaatcc tgcttcaatc cataatcgtt gcaggagctg
    30601 gtcgataaac tcggtgcgcg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30661 atcacccagc caacgctgga aagtatcgat atgggctttt atcccaccga cggttaattc
    30721 atcacggggc cagacgcatg gattttgcgg ttgaccgggc agggtgggga gagcggtgtt
    30781 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30841 aaaaaagccg gcattcgccg gcttcatttt attcgtcgtg cgagattatc gccgggatgg
    30901 tgtcatcctt gcgtagcgtc agaatttcgc agccgttatc agtcaccaca atagtatgct
    30961 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    31021 ggatctcttt tttacccgcg ttgaccattg gctcgatggt gaacgtcatc ccaggtttca
    31081 gtacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31141 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31201 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31261 ggtacaggct ttcttgcgtg atgcggcaca gacgttcgcc catgatggtc ggcttaccga
    31321 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31381 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31441 cattaattga gatgcaaacg gatttcggat aaccgtgata gccgaggcag gcagaaaccg
    31501 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31561 ccggtttaac atatggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31621 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31681 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31741 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattca
    31801 acgtctgatg ctgtacacag cgccaacaat tattggtgtc catgacgtat ttgtggtata
    31861 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31921 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31981 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    32041 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    32101 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32161 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gcttctcgca
    32221 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32281 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32341 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32401 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32461 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32521 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacctgggt attccggtat
    32581 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32641 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32701 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32761 gcacgctcgt acgagccctt atagaccagg tagtacacgt ttggttaggg ggcctgcata
    32821 tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc acatctccga
    32881 ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg agcgtactgg
    32941 cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca tcgagctggc
    33001 aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag gcaacgttgc
    33061 tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc tggaagttaa
    33121 ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag acaaagttct
    33181 ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac agttcgaaga
    33241 agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc gcgttgctgc
    33301 gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggcg ttctggttgc
    33361 tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg ctgcaagcaa
    33421 gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag aataccaggt
    33481 acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga aaatggttga
    33541 aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt tcgttatgga
    33601 accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga ctggcttcat
    33661 ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag cagaagttgc
    33721 tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc ctgagggcgg cttctttttg
    33781 tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg acgtacaatg
    33841 tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat ggctaccaat
    33901 gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct gcagggcact
    33961 gaaggcttcg gtattgatgc aagcatactg gatcgtatgg ctcaggaaat caaagaactg
    34021 gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt ccgtggcgct
    34081 ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat gctggcgacc
    34141 gtaatgaacg gcctggcaat gcgtgatgca ctgcaccgcg cctatgtgaa cgctcgtctg
    34201 atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga agctatcagc
    34261 ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc gttctttacc
    34321 accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt gctgaaagca
    34381 accaaagttg acggcgtgtt taccgctgat ccggcgaaag atccaaccgc aaccatgtac
    34441 gagcaactga cttacagcga agtgctggaa aaagagctga aagtcatgga cctggcggcc
    34501 ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaacatgaa caaaccgggt
    34561 gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga ataattcccg
    34621 tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggatctta cccgtaatat
    34681 gtttaatcag ggctatactt agcacacttc cactgtgtgt gactgtctgg tctgactgag
    34741 acaagttttc aaggattcgt aacgtgatta gcgatatcag aaaagatgct gaagtacgca
    34801 tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt
    34861 ctcccagcct gctggatggc attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc
    34921 agctggcaag cgtaacggta gaagattccc gtactctgaa aatcaacgtg tttgatcgtt
    34981 caatgtctcc ggccgttgaa aaagcgatta tggcgtccga tcttggcctg aacccgaact
    35041 ctgcgggtag cgacatccgt gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc
    35101 tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt aacgtgcgtc
    35161 gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa agagatcagc gaagacgacg
    35221 atcgccgttc tcaggacgat gtacagaaac tgactgatgc tgcaatcaag aaaattgaag
    35281 cggcgctggc agacaaagaa gcagaactga tgcagttctg atttcttgaa cgacaaaaac
    35341 gccgctcagt agatccttgc ggatcggctg gcggcgtttt gctttttatt ctgtctcaac
    35401 tctggatgtt tcatgaagca actcaccatt ctgggctcga ccggctcgat tggttgcagc
    35461 acgctggacg tggtgcgcca taatcccgaa cacttccgcg tagttgcgct ggtggcaggc
    35521 aaaaatgtca ctcgcatggt agaacagtgc ctggaattct ctccccgcta tgccgtaatg
    35581 gacgatgaag cgagtgcgaa acttcttaaa acgatgctac agcaacaggg tagccgcacc
    35641 gaagtcttaa gtgggcaaca agccgcttgc gatatggcag cgcttgagga tgttgatcag
    35701 gtgatggcag ccattgttgg cgctgctggg ctgttaccta cgcttgctgc gatccgcgca
    35761 ggtaaaacca ttttgctggc caataaagaa tcactggtta cctgcggacg tctgtttatg
    35821 gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg atagcgaaca taacgccatt
    35881 tttcagagtt taccgcaacc tatccagcat aatctgggat acgctgacct tgagcaaaat
    35941 ggcgtggtgt ccattttact taccgggtct ggtggccctt tccgtgagac gccattgcgc
    36001 gatttggcaa caatgacgcc cgatcaagcc tgccgtcatc cgaactggtc gatgggacgt
    36061 aaaatttctg tcgattcggc taccatgatg aacaaaggtc tggaatacat tgaagcgcgt
    36121 tggctgttta acgccagcgc cagccagatg gaagtgctga ttcacccgca gtcagtgatt
    36181 cactcaatgg tacgctacca ggacggcagt gttctggcgc agctgggtga accggatatg
    36241 cgtacgccaa ttgcccacac catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg
    36301 ctcgattttt gcaaactaag tgcgttgaca tttgccgcac cggattatga tcgttatcca
    36361 tgcctgaaac tggcgatgga ggcgttcgaa caaggccagg cagcgacgac agcattgaat
    36421 gccgcaaacg aaatcaccgt tgctgctttt cttgcgcaac aaatccgctt tacggatatc
    36481 gctgcgttga atttatccgt actggaaaaa atggatatgc gcgaaccaca atgtgtggac
    36541 gatgtgttat ctgttgatgc gaacgcgcgt gaagtcgcca gaaaagaggt gatgcgtctc
    36601 gcaagctgag gataatccgg ctacagagag tcgcgctatt tgttagcgta gggcttcagt
    36661 gatatagtct gcgccatctg atcgtaagta gttggcttta taaggtcagg tatgccgtgg
    36721 ttttacacgg cttttttttg tataggcttc agtattcctg agtaccgtaa accctgtcag
    36781 ggaataaaaa acgcgtgatg ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc
    36841 atggctgccg tcatgtagcg atcattatgg acggcaatgg tcgctgggca aaaaagcaag
    36901 ggaagattcg tgcctttggg cataaagccg gggcaaaatc cgtccgccgg gctgtctctt
    36961 ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga
    37021 accgaccagc gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg
    37081 aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat tattggcgat accagtcgct
    37141 ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc gctaacagcc gggaataccg
    37201 gtctgacgct gaatattgcg gcgaactacg gtggacgttg ggatatagtc cagggagtca
    37261 ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc agatcagata gatgaagaga
    37321 tgctaaacca gcatgtctgt atgcatgaac tggcccctgt agatttagta attaggactg
    37381 ggggggagca tcgcattagt aactttttgc tttggcaaat tgcctatgcc gaactttact
    37441 ttacagatgt tctctggccc gatttcgatg aacaagactt tgaaggggcg ttaaatgcct
    37501 ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg
    37561 ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg
    37621 cggcgttgtt tctgttgccg ccggtggggt tcgccattgt aacgctggtg gtctgcatgc
    37681 tggcagcgtg ggaatgggga cagcttagcg gttttaccac tcgttcgcag cgagtatggt
    37741 tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt tctgttgccg gaatatcacc
    37801 gaaatattca tcaaccgctg gttgaaatct cactttgggc ttcgctgggt tggtggattg
    37861 tcgcgctatt gctggtgctg ttttacccag gttccgcagc aatctggcgt aactctaaaa
    37921 cattgcgcct tatttttggc gtgctaacca ttgttccctt cttctggggc atgctggcgt
    37981 tacgggcctg gcactacgac gagaatcatt acagtggcgc aatatggctg ctctatgtca
    38041 tgatcctggt atggggcgct gactctggcg catatatgtt tggcaaattg tttggtaaac
    38101 ataagctggc accgaaggtt tctccgggta aaacctggca aggctttata ggtggactcg
    38161 cgaccgcagc agtaatctca tggggttatg gcatgtgggc gaatctcgac gtcgctcccg
    38221 tcaccttact catttgctct attgtcgcag cgttagcctc agtgctcggc gatctgactg
    38281 agagtatgtt taagcgtgaa gcaggaatta aggacagcgg tcatttaatt ccaggacacg
    38341 gtggtatttt agatcgtatt gatagcctga cggctgcggt accggtcttt gcttgcttgt
    38401 tgttactggt attcaggacg ctttaacgga aggtaatatg ctgagttttc tctgggattt
    38461 ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg catgaatttg gtcatttctg
    38521 ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca atagggtttg gtaaggcgct
    38581 ctggcggcga actgataagc tcggcaccga atatgttatc gccctgatcc cgttgggcgg
    38641 ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt ccggaactcc gccaccatgc
    38701 cttcaataat aaatctgtcg gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa
    38761 cttcattttt gctatctttg cctactggct ggtttttatt attggtgtgc ctggcgtacg
    38821 tccggtggtt ggcgaaatag cagccaattc gatagctgcg gaagcacaaa ttgcaccagg
    38881 tacggaacta aaagccgtag atggtatcga aacgcctgat tgggatgccg tgcgtttgca
    38941 gttggtcgat aaaattggcg atgaaagcac caccattaca gtagcgccat ttggcagcga
    39001 ccaacagcgg gatgtaaagc tcgatttacg tcactgggcg tttgaacctg ataaagaaga
    39061 tccggtatct tcgctgggga ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa
    39121 tgtgcaacca aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa
    39181 agtcgatggt cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc gggataaccc
    39241 gggtaaatcc ttagcgttag aaatcgaaag gcagggaagt cccttgtctt tgacattaat
    39301 cccggagagt aaaccgggta atggtaaagc gattggtttt gtcggtattg agccgaaagt
    39361 cattcctttg ccagatgagt ataaagttgt acgccagtat gggccgttca acgccattgt
    39421 cgaagccacg gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt
    39481 gatcaccggt gatgtgaaac tgaacaacct cagtgggccg atctctatcg ccaagggggc
    39541 tgggatgaca gcggaactcg gggttgttta ttacctgccg tttcttgcgc ttattagcgt
    39601 gaacttaggg ataattaacc tgtttccgtt gcccgtactt gacggggggc atctgctgtt
    39661 ccttgcgatc gaaaagatca agggcggacc ggtatccgag cgggttcaag acttttgtta
    39721 tcgcattggc tcgattctgc tggtgctgtt aatggggctt gcacttttca atgatttctc
    39781 tcggttatga gagttagtta ggaagaacgc ataataacga tggcgatgaa aaagttgctc
    39841 atagcgtcgc tgctgtttag cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa
    39901 gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct cagtatgccg
    39961 gtgcgcacag gcgacacggt taatgatgaa gatatcagta ataccattcg cgctctgttt
    40021 gctaccggca actttgagga tgttcgcgtc cttcgtgatg gtgataccct tctggttcag
    40081 gtaaaagaac gtccgaccat tgccagcatt actttctccg gtaacaaatc ggtgaaagat
    40141 gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc
    40201 accaccattg ccgatatcga gaaaggtctg gaagacttct actacagcgt cggtaaatat
    40261 agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca accgtgttga cctaaaactg
    40321 gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta acattgttgg taaccatgct
    40381 ttcaccaccg acgaactgat ctctcatttc caactgcgtg acgaagtgcc gtggtggaac
    40441 gtggtaggcg atcgtaaata ccagaaacag aaactggcgg gcgaccttga aaccctgcgc
    40501 agctactatc tggatcgcgg ttatgcccgt ttcaacatcg actctaccca ggtcagtctg
    40561 acgccagata aaaaaggtat ttacgtcacg gtgaacatca ccgaaggcga tcagtacaag
    40621 ctttctggcg ttgaagtgag cggcaacctt gccgggcact ccgctgaaat tgagcagctg
    40681 actaagatcg agccgggtga gctgtataac ggcaccaaag tgaccaagat ggaagatgac
    40741 attaaaaagc ttctcggtcg ctatggttat gcctatccgc gcgtacagtc gatgcccgaa
    40801 attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg taaccgtttc
    40861 tacgtgcgta agatccgttt tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc
    40921 gaaatgcgtc agatggaagg tgcatggctg gggagcgatc tggtcgatca gggtaaggag
    40981 cgtctgaatc gtctgggctt ctttgaaact gtcgataccg atacccaacg tgttccgggt
    41041 agcccggacc aggttgatgt agtctacaag gtgaaagaac gtaacactgg tagcttcaac
    41101 ttcgggattg gttacggtac tgaaagtggc gtgagcttcc aggctggcgt gcaacaggat
    41161 aactggttag gtacaggtta tgctgttggt atcaacggga ccaaaaacga ttaccagacc
    41221 tatgctgaac tgtcggtaac caacccgtac ttcaccgtag atggcgtaag cctcggtggt
    41281 cgtctcttct ataatgactt ccaggcagat gacgccgacc tgtccgacta taccaacaag
    41341 agttatggta cagacgtgac gttgggcttc ccgattaacg aatataactc gctgcgtgca
    41401 ggtctgggtt atgtacataa ctccctgtcc aacatgcagc cgcaggttgc gatgtggcgt
    41461 tatctgtact ctatgggtga acatccgagc acctctgatc aggataacag cttcaaaacg
    41521 gacgacttca cgttcaacta tggttggacc tataacaagc ttgaccgtgg ttacttcccg
    41581 acagatggtt cacgtgtcaa cctgaccggt aaagtgacca ttccgggatc ggataacgaa
    41641 tactacaaag tgacgttaga cacggcgact tatgtgccga tcgatgacga tcacaaatgg
    41701 gttgttctgg gtcgtacccg ctggggttat ggtgatggtt taggcggcaa agagatgccg
    41761 ttctacgaga acttctatgc cggtggttcc agcaccgtgc gtggcttcca gtccaatacc
    41821 attggtccga aagcagttta cttcccgcat caggccagta attatgatcc ggactatgat
    41881 tacgaatgtg cgactcagga cggcgcgaaa gacctgtgta aatcggatga tgctgtaggc
    41941 ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt tattagcgat
    42001 aagtatgcta actcggttcg tacttctttc ttctgggata tgggtaccgt ttgggataca
    42061 aactgggatt ccagccaata ttctggatat ccggactata gtgatccaag caatatccgt
    42121 atgtctgcgg gtatcgcatt acaatggatg tccccattgg ggccgttggt gttctcctac
    42181 gcccagccgt tcaaaaagta cgatggagac aaggcagaac agttccagtt taacatcggt
    42241 aaaacctggt aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat gactttaggc
    42301 gatcaatata agatcgccgg gccacgcaaa gaactgcacc ctccggtgca aatgggatgg
    42361 taaggagttt attgtgaaaa agtggttatt agcggcaggt ctcggtttag cactggcaac
    42421 ttctgctcag gcggctgaca aaattgcaat cgtcaatatg ggcagcctgt tccagcaggt
    42481 agcgcagaaa accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga
    42541 actgcagcgt atggaaaccg atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc
    42601 gggcagcgat cgcactaagc tggaaaaaga cgtgatggct cagcgccaga cttttgctca
    42661 gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact
    42721 ggttactcgt atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt
    42781 tgttgatgca aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt
    42841 actgaaacag gttaaataag taatgccttc aattcgactg gctgatttag cgcagcagtt
    42901 ggatgcagaa ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc
    42961 tgcacaaaca ggtcacatta cgttcatggt taacccaaaa taccgtgagc atttaggctt
    43021 gtgccaggcg tccgcggttg tcatgaccca ggacgatctt cctttcgcga aaagtgccgc
    43081 gctggtagtg aagaatccct acctgactta cgcgcgcatg gcgcaaattt tagataccac
    43141 gccgcagccc gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg
    43201 taacaacgta tcgattggcg ctaacgcggt gattgactcc ggcgttgaac tgggcgataa
    43261 cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg
    43321 tctctgggcg aacgtaacca tttaccatga gatccagatc ggtcagaatt gcctgatcca
    43381 gtccggaaca gtggtaggcg cagacggctt tggttatgcc aacgatcgtg gtaactgggt
    43441 gaagatccca cagattggtc gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac
    43501 aaccatcgat cgcggcgcgc tggatgacac ggttattggc aatggcgtga tcattgataa
    43561 ccagtgccag attgcacata acgtcgtgat tggcgacaat acggcggttg ccggtggcgt
    43621 cattatggcg ggcagcctga aaattggtcg ttactgcatg atcggcggag ccagcgtaat
    43681 caacgggcat atggaaatat gcgacaaagt gacggttacg ggcatgggta tggtgatgcg
    43741 tcccatcact gaaccaggcg tctattcctc aggcattccg ctgcaaccca acaaagtctg
    43801 gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct
    43861 tgagcgcaag gttaatcaac aagactaacg ttccatcttt tgttcgccaa actttacggc
    43921 ctgtctcatt cttacgattg cggcaggccg tgttattatt gtcgtttctt atattttgac
    43981 aggaagagta tcttgactac taacactcat actctgcaga ttgaagagat tttagaactt
    44041 ctgccgcacc gtttcccgtt cttactggtg gatcgcgtgc tggattttga agaaggtcgt
    44101 tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg ccatttccct
    44161 ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt
    44221 ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc tgtactactt cgccggtatt
    44281 gacgaagcgc gcttcaagcg cccggtcgtg cctggcgatc aaatgatcat ggaagtcact
    44341 ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt cgacggtaaa
    44401 gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg aggcctgata cgtgattgat
    44461 aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcgat tggcgcgaac
    44521 gctcacattg gtcctttttg tatcgttgga ccccatgtcg aaattggtga gggtaccgta
    44581 ctgaaatctc acgttgtcgt gaatggtcat actaaaattg gccgcgataa tgagatttat
    44641 cagttcgcct ccatcggcga agttaaccag gatctgaaat atgctggcga accgacccgc
    44701 gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc
    44761 cagggcggtg gattgacgaa ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt
    44821 gcgcacgatt gtacggtagg taaccgctgt attctcgcca acaacgcaac gctggcgggt
    44881 cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc
    44941 atcattggtg cgcatgtgat ggttggcggc tgctccggtg tggcgcagga cgtccctcct
    45001 tatgtcattg cgcagggtaa ccacgccacg ccgttcggtg tcaatatcga agggctgaag
    45061 cgccgcggat tcagccgtga ggcgattacc gctatccgca atgcgtataa gctgatttat
    45121 cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc ggaaacatat
    45181 ccggaagtga aagcctttac cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa
    45241 tgactgaaca gcgtccatta acgattgccc tggtcgccgg agaaacctcc ggcgatatcc
    45301 tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc tttgttggcg
    45361 ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg
    45421 cggtaatggg cattgttgaa gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg
    45481 atctgacaaa gcgttttggc gaactgaagc cagatgtttt tgttggtatt gatgcgcctg
    45541 acttcaatat taccctcgaa ggtaacctta aaaagcaggg tatcaaaacc attcattacg
    45601 tcagtccatc cgtctgggcg tggcgacaga aacgcgtttt caaaataggc agagccaccg
    45661 atctggtgct cgcatttctg cctttcgaaa aagcgtttta tgacaaatac aacgtaccgt
    45721 gccgctttat cggtcatacc atggctgatg ccatgccatt agatccagat aaaaatgccg
    45781 cccgtgatgt gctggggatc ccgcacgata ctcactgtct ggcattgttg ccgggaagtc
    45841 gtggcgcgga agttgagatg cttagcgccg atttcctgaa aacggcccag cttttgcgcc
    45901 agacgtaccc tgatctggag atcgtggtgc cgctggtgaa tgccaaacgc cgcgagcagt
    45961 ttgaacgcat caaagctgaa gtcgcgccag acctttcagt tcatttgctg gatgggatgg
    46021 gccgtgaggc gatggtcgcc agcgatgcgg cactactggc gtcggggacg gcagccctgg
    46081 agtgtatgct ggcgaaatgc ccgatggtgg tgggatatcg catgaagcct tttaccttct
    46141 ggttggcgaa gcggctggtg aaaactgaat atgtctcgct gccaaatctg ctggcgggca
    46201 gagagttagt caaagaatta ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc
    46261 tgttaccgct gttggcgaac gggaaaacca gccacgcgat gcacgatacc ttccgtgaac
    46321 tgcatcagca gatccgctgc aatgccgatg agcaggcggc acaagccgtt ctggagttag
    46381 cacaatgatc gaatttgtct atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg
    46441 acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg atccttgacc cggcgcgccc
    46501 gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa cgccgtctgg cgctctatga
    46561 agagatcaaa gagaaagcgt tgagctggag tctgggccgc gcggaacccc acgaaatcga
    46621 cgagctgaac attcttcatg cgaccatgct ggcgatgcag cgtgccgtcg ctgggctgca
    46681 tattgcgccg gaatatgtgt tgattgatgg taaccgctgc ccgaaattac cgatgcctgc
    46741 gatggctgtg gtgaaaggcg atagccgcgt accggaaatc agtgccgcgt ctatcctggc
    46801 gaaagtgacg cgcgacgccg aaatggcggc gctggatatt gttttcccgc aatatggttt
    46861 tgcccaacac aaagggtatc caaccgcttt tcatctggaa aaactggctg aacacggcgc
    46921 gaccgaacac catcggcgca gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg
    46981 attcttgtgt cgagattaag taaaccggaa tctgaagatg tctgaaccac gtttcgtaca
    47041 cctgcgggtg cacagcgact actcgatgat cgatggcctg gccaaaaccg caccgctggt
    47101 aaaaaaggcg gcggcgttgg gtatgccagc actggcgatc accgatttca ccaacctttg
    47161 cggtctggtg aagttctacg gagcgggaca tggcgcaggg attaagccta tcgtcggggc
    47221 agattttaac gtccagtgcg acctgctggg tgatgagtta acccacctga cggtactggc
    47281 ggcgaacaat accggctatc agaatctgac gttgctgatc tcaaaagcgt atcagcgcgg
    47341 gtacggtgcc gccgggccga tcatcgatcg cgactggctt atcgaattaa acgaagggtt
    47401 gatccttctt tccggcgggc gcatgggcga cgtcggacgc agtcttttgc gtggtaacag
    47461 cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc gctattttct
    47521 cgagctgatc cgcaccggca ggccggatga agaaagctat ctgcacgcgg cggtggaact
    47581 ggcggaagcg cgcggtttgc ccgtcgtggc gactaacgat gtgcgcttta tcgacagcag
    47641 cgactttgac gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc
    47701 taaacgcccg cgtaactatt cgccgcagca atatatgcgt agcgaagagg agatgtgcga
    47761 gctgtttgcc gacatccccg aagctcttgc caacaccgtt gagatcgcca aacgttgtaa
    47821 cgtaaccgtg cgtcttggtg aatacttcct gccgcagttc ccgaccgggg acatgagcac
    47881 cgaagattat ctggtcaagc gtgcaaaaga gggcctggaa gagcgtctgg cctttttatt
    47941 ccctgatgag gaagaacgtg ttaagcgccg ctcggaatat gacgaacgtc tggagactga
    48001 acttcaggtt atcaaccaga tgggcttccc gggctacttc ctcatcgtta tggaatttat
    48061 ccagtggtcg aaagataacg gtgtaccggt agggccaggc cgtggctccg gcgcaggctc
    48121 actggtggcc tacgcgctga aaatcaccga cctcgatccg ctggaatttg acctgctgtt
    48181 cgaacgtttc cttaacccgg aacgtgtctc catgcctgac ttcgacgttg acttctgtat
    48241 ggagaaacgc gatcaggtta tcgaacacgt agcggacatg tacggtcgtg atgcggtatc
    48301 gcagattatc accttcggta cgatggcggc gaaagcggtg atccgcgacg taggccgcgt
    48361 gctggggcat ccgtacggct ttgtcgatcg tatctcgaaa ctgatcccgc ccgatccggg
    48421 gatgacgctg gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct acgaagcgga
    48481 tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc
    48541 cggtaagcac gccggtgggg tggttatcgc gccgaccaaa attaccgatt ttgcgccgct
    48601 ttattgcgat gaagagggca aacatccggt cacccagttt gataaaagcg acgttgaata
    48661 cgccggactg gtgaagttcg acttccttgg tttgcgtacg ctcaccatca tcaactgggc
    48721 gctggagatg atcaacaagc ggcgggcgaa gaatggcgag ccgccgctgg atatcgccgc
    48781 gatcccgctg gatgacaaga aaagcttcga catgctgcaa cgctcggaaa ccacggcggt
    48841 attccagctt gaatcgcgcg gcatgaagga cctgatcaag cgtctgcaac ctgactgctt
    48901 cgaagatatg atcgcactgg tggcactgtt ccgccctggt ccgttgcagt cagggatggt
    48961 ggataacttt atcgaccgta aacatggtcg cgaagagatc tcctatccgg acgtgcagtg
    49021 gcagcatgaa agcctgaaac cggtactgga gccaacctac ggcattatcc tgtatcagga
    49081 acaggtcatg cagattgccc aggtgctttc tggttatacc ctcggtggcg cggatatgct
    49141 gcgtcgtgcg atgggtaaga aaaagccgga agagatggcg aagcagcgtt ctgtatttgc
    49201 tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg atgaaaatct tcgacctggt
    49261 ggagaaattc gcgggttacg gatttaacaa atcgcactct gcggcctatg ctttggtgtc
    49321 atatcaaacg ttatggctga aagcgcacta tcctgcggag tttatggcgg cggtaatgac
    49381 cgccgatatg gacaacaccg agaaggtggt gggtctggtg gatgagtgct ggcggatggg
    49441 gctgaaaatc ctgccaccag atataaactc cggtctttac catttccacg tcaacgacga
    49501 cggcgaaatc gtgtatggta ttggcgcgat caaaggggtc ggtgaaggtc cgattgaggc
    49561 catcatcgaa gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg
    49621 aaccgacacc aaaaagttaa accggcgagt gctggaaaaa ctgatcatgt ccggggcgtt
    49681 tgaccgtctt gggccacatc gcgcagcgct gatgaactcg ctgggcgatg cgttaaaagc
    49741 ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc gatatgttcg gcgtgctggc
    49801 cgaagagccg gaacaaattg aacaatccta cgccagctgc caaccgtggc cggagcaggt
    49861 ggtattagat ggggaacgtg aaacgttagg cctgtacctg accggacacc ctatcaacca
    49921 gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac
    49981 agaacgtggt aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac
    50041 caagcgcggc aatcgtatcg gtatctgcac gctggatgac cgttccgggc ggctggaagt
    50101 gatgttgttt actgacgccc tggataaata ccagcaattg ctggaaaaag accgcatact
    50161 tatcgtcagc ggacaggtca gctttgatga cttcagcggt gggcttaaaa tgaccgctcg
    50221 cgaagtgatg gatattgacg aagcccggga aaaatatgct cgcgggcttg ctatctcgct
    50281 gacggacagg caaattgatg accagctttt aaaccgactc cgtcagtctc tggaacccca
    50341 ccgctctggg acaattccag tacatctcta ctatcagagg gcggatgcac gcgcgcggtt
    50401 gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg
    50461 cctcattggt tcggagcagg tggaactgga gtttgactaa tacaggaata ctatgagtct
    50521 gaatttcctt gattttgaac agccgattgc agagctggaa gcgaaaatcg attctctgac
    50581 tgcggttagc cgtcaggatg agaaactgga tattaacatc gatgaagaag tgcatcgtct
    50641 gcgtgaaaag agcgtagaac tgacgcgtaa aatcttcgcc gatctcggtg catggcagat
    50701 tgcgcaactg gcacgccatc cacagcgtcc ttataccctg gattacgttc gcctggcatt
    50761 tgatgaattt gacgaactgg ctggcgaccg cgcgtatgca gacgataaag ctatcgtcgg
    50821 tggtatcgcc cgtctcgatg gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga
    50881 aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt accgcaaagc
    50941 actgcgtctg atgcaaatgg ctgaacgctt taagatgcct atcatcacct ttatcgacac
    51001 cccgggggct tatcctggcg tgggcgcaga agagcgtggt cagtctgaag ccattgcacg
    51061 caacctgcgt gaaatgtctc gcctcggcgt accggtagtt tgtacggtta tcggtgaagg
    51121 tggttctggc ggtgcgctgg cgattggcgt gggcgataaa gtgaatatgc tgcaatacag
    51181 cacctattcc gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa
    51241 agcgccgctg gcggctgaag cgatgggtat catcgctccg cgtctgaaag agctgaaact
    51301 gatcgactcc atcatcccgg aaccgctggg cggcgctcac cgtaacccgg aagcgatggc
    51361 ggcatcgttg aaagcgcaac tgctggcgga tctggccgat ctcgacgtgt taagcactga
    51421 agatttaaaa aatcgtcgtt atcagcgcct gatgagctac ggttacgcgt aattcgcaaa
    51481 agttctgaaa aagggtcact tcggtggccc ttttttatcg ccacggtttg agcaggctat
    51541 gattaaggaa ggattttcca ggaggaacac atgaacatca ttgccattat gggaccgcat
    51601 ggcgtctttt ataaagatga acccattaaa gaactggagt cggcgctggt ggcgcaaggc
    51661 tttcagatta tctggccaca aaacagcgtt gatttgctga agtttatcga acataaccca
    51721 cgaatttgcg gcgtgatttt tgactgggat gagtacagtc tcgatttatg tagcgatatc
    51781 aatcagctta atgaatatct cccgctttat gccttcatca acacccactc gacgatggat
    51841 gtcagcgtgc aggatatgcg gatggcgctc tggttttttg aatatgcgct ggggcaggcg
    51901 gaagatatcg ccattcgtat gcgtcagtat accaacgaat atcttgataa cattacccca
    51961 ccgttcacga aagccttgtt tacctacgtc aaagagcgga agtacacctt ttgtacgccg
    52021 gggcatatgg gcggcaccgc atatcaaaaa agcccggttg gctgtctgtt ttatgatttt
    52081 ttcggcggga atacccttaa ggctgatgtc tctatttcgg tcaccgagct tggttcgttg
    52141 ctcgaccaca ccgggccaca cctggaagcg gaagagtaca tcgcgcggac tttcggcgcg
    52201 gaacagagtt atatcgttac caacggaaca tcgacgtcga acaaaattgt gggtatgtac
    52261 gccgcgccat ccggcagtac gctgttgatc gaccgcaact gccataaatc actggcgcat
    52321 ctgttgatga tgaacgatgt agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg
    52381 attctgggtg ggatcccgcg ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct
    52441 gccaccacgc aagcacaatg gccggttcat gcggtgatca ccaactccac ctatgatggc
    52501 ttgctctaca acaccgactg gatcaaacag acgctggatg tcccgtcgat tcacttcgat
    52561 tctgcctggg tgccgtacac ccattttcat ccgatctacc agggtaaaag tggtatgagc
    52621 ggtgagcgtg ttgcgggaaa agtgatcttc gaaacgcaat cgacccacaa aatgctggcg
    52681 gcgttatcgc aggcttcgct gatccacatt aaaggtgagt atgacgaaga ggcgtttaac
    52741 gaagccttta tgatgcatac caccacctcg cccagttatc ccattgttgc ttcggttgag
    52801 acggcggcgg cgatgctgcg tggtaatccg ggcaaacggc tgattaaccg ttcggtggag
    52861 cgggcgctgc attttcgcaa agaggtccag cggttgcggg aagagtctga cggttggttt
    52921 ttcgatatct ggcaaccgcc gcaggtggat gaagccgaat gctggcccgt tgcgcctggc
    52981 gaagagtggc acggctttaa cgatgcggat gccgatcata tgtttctcga tccggttaaa
    53041 gtcactattt tgacaccggg gatggacgaa cagggcaata tgagcgagga ggggatcccg
    53101 gcggcgctgg tggcaaaatt cctcgacgaa cgtgggatcg tagtagagaa aaccggccct
    53161 tataacctgc tgtttctctt tagtattggc atcgataaaa ccaaagcaat gggattattg
    53221 cgtgggttga cggaatttaa gcgctcttac gatctcaacc tgcggatcaa aaatatgctg
    53281 cccgatctct atgctgaaga tcccgatttc taccgcaata tgcgtattca ggatctggcg
    53341 caagggatcc ataagctaat tcgtaaacac gatcttcccg gtttgatgtt gcgggcattc
    53401 gatactttgc cggaaatgat catgacgcca catcaggcat ggcagagaca gattaaaggt
    53461 gaagtagaaa ccattgcgct ggaacaactg gtcggtagag tatcggcaaa tatgatcctg
    53521 ccttatcctc ctggcgtacc gctgttgatg cctggagaaa tgctgaccaa agagagccgc
    53581 acggtactcg attttctact gatgctttgt tccgtagggc aacattaccc cggttttgaa
    53641 acggatattc acggcgcgaa acaggacgaa gacggcgttt accgcgtacg agtcctaaaa
    53701 atggcgggat aacttgccag agcggcttcc gggcgagtaa cgtgctgtta acaaataaag
    53761 gagacgttat gctgggttta aaacaggttc accatattgc gattattgcg acggattatg
    53821 cggtgagcaa agctttctac tgcgatattc ttggtttcac gctgcaaagc gaagtctatc
    53881 gcgaagcgcg cgactcgtgg aaaggggatt tggcgcttaa tgggcaatat gtgattgagc
    53941 ttttctcatt tccgttcccg ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc
    54001 atctggcttt tagcgttgat gacatcgatg cggcagtggc gcaccttgaa agccataacg
    54061 tgaagtgtga agccatccgt gtcgatccat acacgcaaaa acgcttcacc ttctttaacg
    54121 atccggacgg gctgccgttg gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc
    54181 ccggtaacgt gccgggcatt gctactgtaa aatcgcacca tcatgacact cacgctcaat
    54241 agacaacttc tcacctcacg ccagattctg gtggccttta gcggcgggct tgactccacc
    54301 gttctgctgc atcagttggt gcagtggcgg acggaaaatc cgggtgtcgc tctgcgcgct
    54361 attcatgtgc atcacggttt aagtgccaat gccgatgcct gggttacgca ttgcgaaaac
    54421 gtctgccaac agtggcaggt gccgctggtg gtcgaacgcg tacaacttgc gcaagagggg
    54481 ctggggattg aggcccaggc gcggcaggcg cgttatcagg tatttgcccg caccttgttg
    54541 cccggtgaag tgctggtcac cgcgcaacat ctcgacgatc aatgtgaaac ttttctgctg
    54601 gcgctaaaac gcggtagcgg ccctgccggg ctttcggcta tggcggaagt ttcggagttt
    54661 gccggaacac ggcttatccg cccgttgctt gcccgcacgc ggggggaact ggcgcagtgg
    54721 gcattggcgc atggtttact ctggattgaa gacgaaagta atcaggacga ctcatacgat
    54781 cgtaactttc tgcgcctgcg cgtagtgccg ttattgcagc agcgttggcc gcattttgcc
    54841 gaaacaacgg cccgcagcgc cgcactttgt gctgaacaag agagcctgct ggatgaactg
    54901 ctggcagatg atttagcaca ctgtcaatcg ccgcagggga cgctgcagat tgtgccaatg
    54961 ctggcgatga gtgatgcccg ccgcgcggcg attatccgcc gctggctggc agggcagaat
    55021 gcaccgatgc cttcccgcga cgcgttggtg aggatctggc aggaagtggc gctggcgcgg
    55081 gaagatgcct ccccctgttt acgtttgggc gcgtttgaaa tccgacgcta tcagtcgcaa
    55141 ctgtggtgga ttaaatccgt caccgggcaa agcgaaaaca ttgtgccgtg gcagacgtgg
    55201 cttcaaccgc tggaattacc ggcggggctg ggaagtgtac agcttaatgc gggaggtgct
    55261 attcgccctc cgcgtgcaga cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg
    55321 catattgtcg ggcgtaacgg cggacgtaag ctaaagaaaa tctggcaaga gctgggcgtg
    55381 ccgccgtggc tacgtgacac cacgccactg ctgttttatg gcgaaacgct gattgcggcg
    55441 gcaggggtat ttgtgacgca agaaggtgtg gctgaaggtg agaatggcat aagttttgtc
    55501 tggcagcgaa acgcttagtt aagtgaaggc cggatatgac gcattaagcg tcgcatccgg
    55561 cgaaagtcaa tcaggactcg ctcaccacca ccgtaccgat ttccgggtgg ctaaagctgg
    55621 taattttatc cagacgtagc tcacgggttt cgccagcggc ctcgacgacc agatactcca
    55681 catttttgcg ggagactaaa tcgctggctt ttgcctgcaa tttttcgcca tctttcagct
    55741 caagtgtcag cattaaatga tgctggcagg cgagctcaag attatcgtaa tcatcacaat
    55801 tgattggttg atacgtatca ttcattgaca taatcgctca ccagtaagtt tgccgcagcg
    55861 tatgctgctt tttccctgac agcctcagaa agggcgtcgt cggcagccat ttcattcagc
    55921 actttcaaaa cgcagcccag cgcgtccgga acgtatccta agtctccgct ggcgatttcc
    55981 gcgtaccgct tgcgtattaa ctcacaatat ttttccacat gccctcctgt cagcactctg
    56041 acttaaccgt ggatgcaagt ctaagcctac gaagataaac tctgtttcgc aaggtgacta
    56101 taccacactc atttctgcaa tatcagcgcc gcaactgcac gtattccgtt acaatggcct
    56161 cctgattcga aaggagtttt cttatggcgc ttaaagcgac aatttataaa gcgacggtta
    56221 atgtggccga tctcgaccgc aaccagtttc tcgatgcctc tctgacgctg gcgcgccatc
    56281 cttcagaaac ccaggagcgt atgatgctgc gcttgctggc gtggctgaaa tatgctgatg
    56341 aacgtctgca atttacccgt ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta
    56401 acgatcacct gggcattgat ttgtggattg agctggggct gccggatgag cggcggatta
    56461 agaaagcctg cacccaggcc gcagaagtgg cgctgtttac ctataatagt cgggcggcgc
    56521 aaatctggtg gcagcaaaat cagagcaaat gtgtgcagtt tgccaatctt tccgtctggt
    56581 atctggacga tgaacaactg gcgaaagtaa gcgcctttgc cgatcgtacc atgacgctgc
    56641 aggcaacgat tcaggatggc gtgatctggt tatcggatga taagaataat ctggaagtga
    56701 acttaaccgc ctggcaacaa ccttcatgat tgtgatttcc cgacatgttg ctattcccga
    56761 tggtgagctt gagatcaccg ccattcgtgc gcagggcgcg ggcgggcagc atgttaataa
    56821 gacctcaacg gctattcatc tgcgttttga cattcgggcg tccagcctgc cagagtatta
    56881 caaagagcat ctgctcgccg ccagccatca tttgatcagc agtgatggcg tgattgtcat
    56941 taaggcacag gaataccgca gtcaggaact gaaccgcgaa gcagctctgg cccggctggt
    57001 ggctgtgatt aaagaattaa caacagaaca aaaagcccga cgacccacgc ggcccacccg
    57061 tgcatcgaaa gagcgcaggc tggcatcgaa agcacaaaaa tcaagcgtga aggcgatgcg
    57121 cggcaaagtg cgcagcggtc gggaataaaa agaaggaatg gatggtgaaa aaagcgatag
    57181 tgacagcgat ggctgtaatc agcctcttta ctctgatggg ttgtaataat cgggccgaag
    57241 tcgatacgct ttctccggcg caggctgccg aattgaaacc gatgccgcaa agttggcgcg
    57301 gcgtgctgcc gtgtgccgat tgcgaaggaa tcgaaacctc tctgttcctc gaaaaagacg
    57361 gaacatgggt gatgaatgag cgttatctcg gtgctcgtga agaaccttcc tccttcgctt
    57421 cctacggtac atgggcgcga accgctgaca agctggtatt aaccgacagc aaaggtgaaa
    57481 agtcatatta tcgggcgaaa ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccga
    57541 ttgaatcgca gttcaactat acgctggaag cggcacaatc cagtttaccc atgacgccga
    57601 tgaccctgcg gggcatgtat ttttatatgg ctgatgcggc gaccttcact gattgcgcga
    57661 ccggaaaacg tttcatggta gcgaataacg cagagctgga gcgtggctac ctggctgcgc
    57721 gcggtcacag tgaaaaaccg gtgttactgt cagtagaagg tcactttacg cttgaggcta
    57781 atccggatac cggtgcgcca accaaagtat tagcacccga tacggcaggg aaattttacc
    57841 caaaccagga ttgcagtagt ttggggcagt aacccgtctt gagacagaaa caaacgcaaa
    57901 acaggccaga aggatatatt tcaacatttt gaatttgcac gttttttgta ggccggataa
    57961 ggcgtttacg ccgcatccgg caatggtgct caacgcctga tgcgacgctg gcgcgtctta
    58021 tcatgcctac aacccccctc ataccttaag gcggataagg catttacttt atcaccgggt
    58081 cagcccaaca aagcgacccg ccttgatata aatccctggc ttcaggtgcc caacatactg
    58141 taactgcgtt tctggcttaa acgcagagac atcaccaata cgcatatgca tcagatcggc
    58201 agggctaatc cagcccgatt gcgccagcgt aagcggatgc ccggctttgg caaatgcatc
    58261 agtgacaaat tccgaacaaa accacgactt tttgtctcct tcgcccacac tgcttaactg
    58321 cgctttcgcc aggccgctga cgcactgttg gcgaaaatcc tcggagaacg gattcagtga
    58381 gcacatctgg cgagtcacca taaaggggat aaattcgaca atgccgcgat agttataacc
    58441 gctatctttg attttattgg cgaacgcggt gatttctgtg gcttgttgcg gggtaagatc
    58501 cgggactcgt aagacgaaaa gtttatcact atgcttcatc gcttttttaa gggaaacaat
    58561 ctggacgcca gcgcctgtcg cttctgcaac gttattttca cccagataga ttgcaacgtg
    58621 actcacagag gaggtgctga aaacgcggat cccaaatgag gttaccccca ggcttgagga
    58681 gaacagcaaa tcgccgggtt tgagatcagg taccgttatt tcttttattg attgttcggt
    58741 gaaagagctt tgatgctgga atttgacagc ccatgttttt gcctcaacat ctaccgctgt
    58801 ggcagaggag tctggctggc tgatatcaac ggtacaggcc gaaagtaaaa gaaagcaggg
    58861 gagaagcagg cggtagtacg cctttggttt atccatttta tacaatccat gtaaaaaagg
    58921 gccctgaaat tcaggaccct ttctggcatc agcctttaat ctgtttcacc agatattcga
    58981 cgatgtcacc agtcttaatt aactgtttct cgccgttgcg acgatattta tattcgatat
    59041 cgtcgttgtc gaggttacgg tcgcccagca caatagtgtg cggaataccg atcagttcca
    59101 tatcggcaaa catcacgccc ggacgttctt ttcggtcatc cagcagcact tcgatacctt
    59161 gtgcacgcag ttcgctgtac agtttctcag caagctcttg tacgcggaag gatttgtgca
    59221 tgttcatcgg cagaatcgcc acctggaacg gcgcgatagc gtcaggccat acgatgccgc
    59281 gttcgtcgta gttctgctca atcgccgcag ctactacacg cgttaccccg ataccgtagc
    59341 aacccatcgt caggatttgg ttacggccat cttcaccctg tacggaggct ttcagtgctt
    59401 cggagtactt ggtacccagc tggaagatgt gaccaacttc gataccacgt ttgatcagca
    59461 gcgtaccctg gccatccggg cttggatcgc cagccaccac gttacggata tccgcaactt
    59521 ccggggtagc gacatcgcga tcccagttga tgccgaagta gtgtttacca tcgatgttag
    59581 caccagcagc gaaatcactc atcgccgcaa cggtacggtc aatcaccacc ggaatcggca
    59641 tgtttaccgg acccagtgaa cccggaccgg ctttaaccac ggcacgaatt tcttcttcgg
    59701 tcgcgaaagt cagcgggctg gcaacctgcg gcagtttttc tgctttaact tcgttcagtt
    59761 cgtggtcacc gcgcaccagc agcgcaacca gcgggaagct actgccttca accgctttaa
    59821 ccagcagagt cttaaccgtt ttctcaatcg gcagattgaa ctgttcaacc agttccgcga
    59881 tggttttcgc gttcggcgta tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt
    59941 ctttcggcgc gatagcttct gccagttcaa tgttcgctgc atagtcagag gtgtcggaga
    60001 agaccacatc gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc
    60061 cgatagaacc ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt
    60121 tgctgtaggc cgcatacatt gcatcgtagg tttcctgcag ggattcctga gaagtatgga
    60181 aagagtaagc atctttcatc aggaattcgc gggaacgcat gacgccgaaa cgcggacgca
    60241 cttcgtcgcg gaacttggtc tggatctgat agaagttcag cggcagctgt ttgtaagagc
    60301 taagctcgtt acgaatcagg tcagtgataa cttcttcatg agttgggccg agtacgaacg
    60361 gacgctcgcc acggtcaaca aaacgcagca gttccggacc gtactgttcc caacgaccac
    60421 tctcttgcca caaatcggct ggctgaacca ccggcatcga cacctcgatc gcaccggcgt
    60481 tgttcatctc ttcacgcacg atgttttcga cttttttcag aacgcgcacg ccggtcggca
    60541 gccaggtata taacccggag gccagcttgc ggatcatccc ggcgcgcagc atcagctgat
    60601 ggctgatcac ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg tattggctag
    60661 tacgcatgtt gttacggttc cagttggaag gtagaacagg ctcaaggcga gcctgggaca
    60721 aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta
    60781 acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa
    60841 atcatgtagc cagacggcat aggttttgcc cgtttcctca cctttacgat aggccgggcg
    60901 cgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac gcttctccag
    60961 cgtcaaaagc tgcttttcga cctctgcggt aaaactcacc gccatctctg cagctggcgc
    61021 actttgcgca tagctggcac tggcatcggg aagcgattcg gcaaagggga gatacggttt
    61081 gatatccact accggcgtac catcgaccag atccagactg ccgagcttca gaatcacgct
    61141 gtctttatgg caaacaacct ctttcagctc taccagcgac atgccaattg ggttagggcg
    61201 gaaagtagag cgtgttgcga aaacccccat tctggcgtta ccgccgaggc gcggtggacg
    61261 cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagga tccataaatg
    61321 gctgaacgct tccaggccgc gaacggcgtc ggcctggttg tagggagcaa tgagatgcag
    61381 ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaact tttctttata
    61441 gggcgagcga ataacgccta tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta
    61501 agcgcagagc cgatacatac agcctgacga tagcagcccg gcgtaccgct ggtgacttcg
    61561 cagctatgca gtaataccgc attggctttc attttcgagg cgttgatttg catccgctta
    61621 cgcgcggttg gaatgctcgg cggagagtcc tgattagagg cctggcaaga gtcgccactg
    61681 acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt ataaattcgg
    61741 accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg gggtgcagtg
    61801 ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag catgagtgct
    61861 actaaacaga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa aacgtcaata
    61921 ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga atgatattac
    61981 aaattgtgga aacagcctaa aaattaccag cctttaacag cgccgccgtt aaacactttg
    62041 tttgctgctt cgtaaacttc gtcagactga taagcctgga cgaatttctt cacgttctcg
    62101 gcgtctttgt tatcttcacg cgttacgatc aggtttacgt acggggagtc tttatcttcg
    62161 acaaagatac cgtttttcgc tggagtcagg ccaatctggc tggcataggt ggtgttaata
    62221 actgccagag cgatttgcgc gtcgtccaga gaacgcggca gttgcggtgc ttccagttca
    62281 acaattttca gattttttgg gttctcaaca acatcaagaa cggtcggcag caggccaacg
    62341 ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg accaaggtta
    62401 gttgggtcgt ttggcacggc aacctgcgaa ccatcctgca gttcatccag tgatttgatt
    62461 ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac cagtttgtag
    62521 ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata
    62581 tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt tgaaggttac cagctcaacg
    62641 tccaggccat atttgtcttt cgcaactttc tgcgcgactt ctgctacctg ctgttcggca
    62701 ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc gcagcctacc
    62761 agtgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt gaacgccata
    62821 ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt gacagcccgg
    62881 acgatgcggt cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa taccagtacc
    62941 gtattcatca ccgtcgcgtt atagccgatg tagccatact gatagccaat ctgacctaaa
    63001 ccaccggcac cgactgcacc acccatcgcg gaataaccga ccagggtaat cagggtgata
    63061 gttgccgcat tcaccagacc cggcaacgct tccggtaaca gcactttacg gacgatctgc
    63121 attggcgtgg cacccattgc gcgggaagct tcaattaacc cggttgggat ctccagcaga
    63181 gcgttctcga ccatacgggc aataaacggt gctgcaccaa cggttaacgg aacaattgct
    63241 gcctgcaaac caatcgatgt accgacaata acgcgggtaa acggaatcat ccataccagc
    63301 aagataatga acgggatgga acggaaaatg ttcacaatcg cagaaacggt acgatacagc
    63361 ttcgcgttag caataatttg ccccggacgc gtgacataaa gcaggacgcc aaccggcaga
    63421 ccaatcacaa acccaaaaaa tccggatacg aaggtcattg ccagcgtttc ccatacgcca
    63481 cgaaccagca gccacatcat cggctcagac ataacccagt acctctactt ttacatggtg
    63541 ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag
    63601 catgatgccg aacttaacgc caccggcgta atccatctgc gcgctaataa tgttgttgtt
    63661 gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt gaccggtaaa
    63721 ctccagacgc agcatcggca cgcagtcagt aaatggctcc gcttgcagac gttcctggta
    63781 atcttccggg atatccagat gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt
    63841 cggatgcgag aacacttcac ttaccgtgtc ctgctcgatc agttcgccat tactgataac
    63901 cgccacgcaa tcacaaatgc gcttcacaac gtccatttcg tgggtgatca acagaatcgt
    63961 caaacccaga cggcggttaa tgtctttcag cagttcgaga atagaacgtg tcgttgccgg
    64021 gtctagcgcg ctggtggctt catcacacag caatactttg ggattgctgg ctaatgcacg
    64081 ggcaatcgcc acacgttgtt tctgcccacc ggaaagattc gacgggtagc tatcatgctt
    64141 atctccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatct cgtctttcgg
    64201 tgtattgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg aagagagcag
    64261 gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact cggattctga
    64321 cagcgtggtc agttcctggc catcgaccag cacactaccc tcggttgggc gctccagcag
    64381 gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa cgccataaat
    64441 ttgcccagct ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg
    64501 gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat cgtcattaag
    64561 ttagtcgtgg catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag
    64621 gcatccagac gtctaaatca atcaggttta tgcgaagagc actttcttgc aggtcgaaac
    64681 atgcgatact agcgtcacat gccttattaa ggagctataa aaggtggcga agagcgtacc
    64741 cgcaattttt cttgaccgtg atggcaccat taatgtcgat cacggctatg tccatgagat
    64801 cgacaacttt gaatttatcg acggtgttat tgacgccatg cgcgagctta aaaaaatggg
    64861 ctttgcgctg gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc
    64921 acagtttgaa acgctgaccg agtggatgga ctggtcgctg gcggaccgag atgtcgatct
    64981 ggatggtatc tattattgcc cgcatcatcc gcagggtagt gttgaagagt ttcgccaggt
    65041 ctgcgattgc cgcaaaccac atccggggat gcttttgtca gcacgcgatt atttgcatat
    65101 tgatatggcc gcttcttata tggtgggcga taaattagaa gatatgcagg cagcggttgc
    65161 ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa cctattacac ctgaagcaga
    65221 aaacgcggca gattgggtgt taaatagcct ggcagacctg ccgcaagcga taaaaaagca
    65281 gcaaaaaccg gcgcaatgat taaaagatga gcggttgaaa taaaaatgca tttttccgct
    65341 tgtcttcctg agccgactcc ctataatgcg cctccatcga cacggcggat gtgaatcact
    65401 tcacacaaac agctggttcg gttgaagag
//
LOCUS       DAZUCE010000005        58640 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZUCE010000005 DAZUCE010000000
VERSION     DAZUCE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.005"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..107
                     /gene="yigG"
                     /locus_tag="ACHY03_000279"
     CDS             <1..107
                     /gene="yigG"
                     /locus_tag="ACHY03_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFS3903566.1"
                     /translation="EDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            121..501
                     /gene="yigF"
                     /locus_tag="ACHY03_000280"
     CDS             121..501
                     /gene="yigF"
                     /locus_tag="ACHY03_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFS3903567.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(544..1494)
                     /gene="corA"
                     /locus_tag="ACHY03_000281"
     CDS             complement(544..1494)
                     /gene="corA"
                     /locus_tag="ACHY03_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFS3903568.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1646..1702)
                     /gene="ysgD"
                     /locus_tag="ACHY03_000282"
     CDS             complement(1646..1702)
                     /gene="ysgD"
                     /locus_tag="ACHY03_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFS3903569.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1864..2628
                     /gene="yigE"
                     /locus_tag="ACHY03_000283"
     CDS             1864..2628
                     /gene="yigE"
                     /locus_tag="ACHY03_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFS3903570.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDENYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRILPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKAKLDVE
                     QLLYLDGTISHMYVKGGAIPWQRYSFVTMISVEQKS"
     gene            complement(2764..4926)
                     /gene="uvrD"
                     /locus_tag="ACHY03_000284"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(2764..4926)
                     /gene="uvrD"
                     /locus_tag="ACHY03_000284"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFS3903571.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMSLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMAENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(5010..5726)
                     /gene="yigB"
                     /locus_tag="ACHY03_000285"
     CDS             complement(5010..5726)
                     /gene="yigB"
                     /locus_tag="ACHY03_000285"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFS3903572.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(5726..6622)
                     /gene="xerC"
                     /locus_tag="ACHY03_000286"
     CDS             complement(5726..6622)
                     /gene="xerC"
                     /locus_tag="ACHY03_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFS3903573.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6619..7326)
                     /gene="yigA"
                     /locus_tag="ACHY03_000287"
     CDS             complement(6619..7326)
                     /gene="yigA"
                     /locus_tag="ACHY03_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFS3903574.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7323..8147)
                     /gene="dapF"
                     /locus_tag="ACHY03_000288"
     CDS             complement(7323..8147)
                     /gene="dapF"
                     /locus_tag="ACHY03_000288"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFS3903575.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDLDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8184..8387)
                     /gene="yifL"
                     /locus_tag="ACHY03_000289"
     CDS             complement(8184..8387)
                     /gene="yifL"
                     /locus_tag="ACHY03_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS3903576.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8576..10054
                     /locus_tag="ACHY03_000290"
     CDS             8576..10054
                     /locus_tag="ACHY03_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFS3903577.1"
                     /translation="MHYRNVVCLLACSLFLSSAWGCRLDAPEHNIYQKQGKGVVYLRP
                     YKETNLSLPDVNYKSLRRLPNLLIDPTTLDEWDKEPPQTDLTTDYLYEGAQAWYPHYS
                     WYSDGRNILYAGEVVQNPPGTPPVDVASFKAWGDFAADKHSLYFEGKRTDDNGGRNSL
                     DIKTLHQVEFRPPWAPDLLGLILRDANFLYINGHRLADPESFRVLAQKSWDQRGKFST
                     TFNPCVAVPFGPWDTLARTRTKILINGEQLVADPDTFSVVRWMPGSLLTWRDKNGLQR
                     KVLDKENLAWDEDLTKHCLDFSLLEKKVFWRQGPDCKQEELPGLDPEQFHPISDAVAQ
                     YQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPYHSGLHV
                     FESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLWRYRTADPKQV
                     RLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            10051..11376
                     /locus_tag="ACHY03_000291"
     CDS             10051..11376
                     /locus_tag="ACHY03_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFS3903578.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYGE
                     DEKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSNNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NAVHLTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDHSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTRVREENVRELHNRDIIIPLTDESCRNAAAEVQTLKP"
     gene            11424..11744
                     /gene="cyaY"
                     /locus_tag="ACHY03_000292"
     CDS             11424..11744
                     /gene="cyaY"
                     /locus_tag="ACHY03_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFS3903579.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(11784..14330)
                     /gene="cyaA"
                     /locus_tag="ACHY03_000293"
     CDS             complement(11784..14330)
                     /gene="cyaA"
                     /locus_tag="ACHY03_000293"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFS3903580.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            14717..15658
                     /gene="hemC"
                     /locus_tag="ACHY03_000294"
     CDS             14717..15658
                     /gene="hemC"
                     /locus_tag="ACHY03_000294"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFS3903581.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            15655..16395
                     /gene="hemD"
                     /locus_tag="ACHY03_000295"
     CDS             15655..16395
                     /gene="hemD"
                     /locus_tag="ACHY03_000295"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFS3903582.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDSAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            16417..17610
                     /gene="hemX"
                     /locus_tag="ACHY03_000296"
     CDS             16417..17610
                     /gene="hemX"
                     /locus_tag="ACHY03_000296"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFS3903583.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPQAAPQADTPAA
                     APQGE"
     gene            17613..18809
                     /gene="hemY"
                     /locus_tag="ACHY03_000297"
     CDS             17613..18809
                     /gene="hemY"
                     /locus_tag="ACHY03_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFS3903584.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAVMRRDGLMLTL
                     QNNPPQ"
     gene            19488..21143
                     /gene="aslA"
                     /locus_tag="ACHY03_000298"
     CDS             19488..21143
                     /gene="aslA"
                     /locus_tag="ACHY03_000298"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFS3903585.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLAAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(21311..22546)
                     /gene="aslB"
                     /locus_tag="ACHY03_000299"
     CDS             complement(21311..22546)
                     /gene="aslB"
                     /locus_tag="ACHY03_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFS3903586.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRYYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(22693..22769)
                     /locus_tag="ACHY03_000300"
     tRNA            complement(22693..22769)
                     /locus_tag="ACHY03_000300"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22733..22735),aa:Pro,seq:tgg)
     gene            complement(22812..22898)
                     /locus_tag="ACHY03_000301"
     tRNA            complement(22812..22898)
                     /locus_tag="ACHY03_000301"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22862..22864),aa:Leu,seq:cag)
     gene            complement(22919..22994)
                     /locus_tag="ACHY03_000302"
     tRNA            complement(22919..22994)
                     /locus_tag="ACHY03_000302"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22959..22961),aa:His,seq:gtg)
     gene            complement(23053..23129)
                     /locus_tag="ACHY03_000303"
     tRNA            complement(23053..23129)
                     /locus_tag="ACHY03_000303"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23093..23095),aa:Arg,seq:ccg)
     gene            complement(23232..24617)
                     /gene="yifK"
                     /locus_tag="ACHY03_000304"
     CDS             complement(23232..24617)
                     /gene="yifK"
                     /locus_tag="ACHY03_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3903587.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(24808..25548)
                     /gene="wecG"
                     /locus_tag="ACHY03_000305"
     CDS             complement(24808..25548)
                     /gene="wecG"
                     /locus_tag="ACHY03_000305"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFS3903588.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGEL"
     gene            complement(25551..26903)
                     /gene="wzyE"
                     /locus_tag="ACHY03_000306"
     CDS             complement(25551..26903)
                     /gene="wzyE"
                     /locus_tag="ACHY03_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFS3903589.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAVAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(26900..27979)
                     /gene="rffT"
                     /locus_tag="ACHY03_000307"
                     /gene_synonym="wecF"
     CDS             complement(26900..27979)
                     /gene="rffT"
                     /locus_tag="ACHY03_000307"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFS3903590.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTVLVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLKLFSEENLRVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(27976..29226)
                     /gene="wzxE"
                     /locus_tag="ACHY03_000308"
     CDS             complement(27976..29226)
                     /gene="wzxE"
                     /locus_tag="ACHY03_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFS3903591.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(29228..30358)
                     /gene="rffA"
                     /locus_tag="ACHY03_000309"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(29228..30358)
                     /gene="rffA"
                     /locus_tag="ACHY03_000309"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFS3903592.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKYNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDSYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(30363..31037)
                     /gene="rffC"
                     /locus_tag="ACHY03_000310"
                     /gene_synonym="wecD"
     CDS             complement(30363..31037)
                     /gene="rffC"
                     /locus_tag="ACHY03_000310"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFS3903593.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(31015..31896)
                     /gene="rfbA"
                     /locus_tag="ACHY03_000311"
     CDS             complement(31015..31896)
                     /gene="rfbA"
                     /locus_tag="ACHY03_000311"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFS3903594.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(31915..32982)
                     /gene="rffG"
                     /locus_tag="ACHY03_000312"
     CDS             complement(31915..32982)
                     /gene="rffG"
                     /locus_tag="ACHY03_000312"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFS3903595.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(32982..34244)
                     /gene="wecC"
                     /locus_tag="ACHY03_000313"
     CDS             complement(32982..34244)
                     /gene="wecC"
                     /locus_tag="ACHY03_000313"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFS3903596.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(34241..35371)
                     /gene="wecB"
                     /locus_tag="ACHY03_000314"
     CDS             complement(34241..35371)
                     /gene="wecB"
                     /locus_tag="ACHY03_000314"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFS3903597.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(35427..36473)
                     /gene="wzzE"
                     /locus_tag="ACHY03_000315"
     CDS             complement(35427..36473)
                     /gene="wzzE"
                     /locus_tag="ACHY03_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFS3903598.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(36485..37588)
                     /gene="wecA"
                     /locus_tag="ACHY03_000316"
     CDS             complement(36485..37588)
                     /gene="wecA"
                     /locus_tag="ACHY03_000316"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFS3903599.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(37828..39087)
                     /gene="rho"
                     /locus_tag="ACHY03_000317"
     CDS             complement(37828..39087)
                     /gene="rho"
                     /locus_tag="ACHY03_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFS3903600.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(39172..39273)
                     /gene="rhoL"
                     /locus_tag="ACHY03_000318"
     CDS             complement(39172..39273)
                     /gene="rhoL"
                     /locus_tag="ACHY03_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFS3903601.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(39414..39743)
                     /gene="trxA"
                     /locus_tag="ACHY03_000319"
     CDS             complement(39414..39743)
                     /gene="trxA"
                     /locus_tag="ACHY03_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFS3903602.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            39874..41139
                     /gene="rhlB"
                     /locus_tag="ACHY03_000320"
     CDS             39874..41139
                     /gene="rhlB"
                     /locus_tag="ACHY03_000320"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFS3903603.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            41275..42759
                     /gene="gppA"
                     /locus_tag="ACHY03_000321"
     CDS             41275..42759
                     /gene="gppA"
                     /locus_tag="ACHY03_000321"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFS3903604.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQDWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(42806..44827)
                     /gene="rep"
                     /locus_tag="ACHY03_000322"
     CDS             complement(42806..44827)
                     /gene="rep"
                     /locus_tag="ACHY03_000322"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFS3903605.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            45044..45493
                     /locus_tag="ACHY03_000323"
     CDS             45044..45493
                     /locus_tag="ACHY03_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFS3903606.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLKRGMHVEIASNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            45692..45973
                     /gene="ppiC"
                     /locus_tag="ACHY03_000324"
     CDS             45692..45973
                     /gene="ppiC"
                     /locus_tag="ACHY03_000324"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFS3903607.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(46019..47494)
                     /gene="ilvC"
                     /locus_tag="ACHY03_000325"
     CDS             complement(46019..47494)
                     /gene="ilvC"
                     /locus_tag="ACHY03_000325"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFS3903608.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQSKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            47644..48537
                     /gene="ilvY"
                     /locus_tag="ACHY03_000326"
     CDS             47644..48537
                     /gene="ilvY"
                     /locus_tag="ACHY03_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFS3903609.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(48589..50133)
                     /gene="ilvA"
                     /locus_tag="ACHY03_000327"
     CDS             complement(48589..50133)
                     /gene="ilvA"
                     /locus_tag="ACHY03_000327"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFS3903610.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(50136..51986)
                     /gene="ilvD"
                     /locus_tag="ACHY03_000328"
     CDS             complement(50136..51986)
                     /gene="ilvD"
                     /locus_tag="ACHY03_000328"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFS3903611.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(52051..52980)
                     /gene="ilvE"
                     /locus_tag="ACHY03_000329"
     CDS             complement(52051..52980)
                     /gene="ilvE"
                     /locus_tag="ACHY03_000329"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFS3903612.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(53000..53263)
                     /gene="ilvM"
                     /locus_tag="ACHY03_000330"
     CDS             complement(53000..53263)
                     /gene="ilvM"
                     /locus_tag="ACHY03_000330"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFS3903613.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(53260..54906)
                     /gene="ilvG"
                     /locus_tag="ACHY03_000331"
     CDS             complement(53260..54906)
                     /gene="ilvG"
                     /locus_tag="ACHY03_000331"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFS3903614.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(54909..54959)
                     /gene="ilvX"
                     /locus_tag="ACHY03_000332"
     CDS             complement(54909..54959)
                     /gene="ilvX"
                     /locus_tag="ACHY03_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFS3903615.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(55046..55144)
                     /gene="ilvL"
                     /locus_tag="ACHY03_000333"
     CDS             complement(55046..55144)
                     /gene="ilvL"
                     /locus_tag="ACHY03_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFS3903616.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            55497..57017
                     /gene="yifB"
                     /locus_tag="ACHY03_000334"
     CDS             55497..57017
                     /gene="yifB"
                     /locus_tag="ACHY03_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFS3903617.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(57042..57380)
                     /gene="maoP"
                     /locus_tag="ACHY03_000335"
     CDS             complement(57042..57380)
                     /gene="maoP"
                     /locus_tag="ACHY03_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFS3903618.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            57499..58338
                     /gene="hdfR"
                     /locus_tag="ACHY03_000336"
     CDS             57499..58338
                     /gene="hdfR"
                     /locus_tag="ACHY03_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFS3903619.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(58434..58509)
                     /locus_tag="ACHY03_000337"
     tRNA            complement(58434..58509)
                     /locus_tag="ACHY03_000337"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58474..58476),aa:Trp,seq:cca)
     gene            complement(58518..58594)
                     /locus_tag="ACHY03_000338"
     tRNA            complement(58518..58594)
                     /locus_tag="ACHY03_000338"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58558..58560),aa:Asp,seq:gtc)
BASE COUNT        14426 a        15790 c        14852 g        13572 t
ORIGIN      
        1 ccgaagattt tgcctgttca ggcaatggtt cgatgatttt tttgatttgt tatttttcca
       61 ctttcagtat gctaatgctt gcacctactg attcatcaaa acaatagtta tggagtattt
      121 atgagtaagg aatatatgaa cgatggttca ttaagtgaaa aatggaaata ccgatttaat
      181 ttttatgatc aacatggctt tcctggattc tggggagcaa caccagaata taaagcagca
      241 tttaaagcat taaaagtaag acaacgatta actattcaaa tgaatttcat tgctttcttc
      301 tgttcctgga tctatttatt tgttctgggc ttatggaaaa aagcaattat agttctatta
      361 cttggcattc tatctctatt tgttggtgca ttaattggcg ttaacattct gggaattgcc
      421 gtagcagcct atgttgctgt taacacgaac aaatggttct atgagaaaga ggtaaaaggg
      481 ttaaatacct ggagcctttg aataacaggt ggcttagcca gactaagcca ccactctcgt
      541 tttttacaac cagttcttcc gcttaaagta cagatacggt gccaggcccg cgaggatcat
      601 aaagataatc gcgcctgggt agccgaaact ccacttcagt tctggcataa actcaaagtt
      661 catcccgtag ctggaagcaa ccagcgttgg cggcaggaac acgacggata ccaccgagaa
      721 aattttgatg atgcggttct gctcgatgtt gataaaaccc attgccgcct gcatcaggaa
      781 gttcaccttc tggaacaggg attcgttatg cggcagcagg gattcgatat ctcgcaggat
      841 ttcacgcgcc tgctccagtt gcccacccgg taagcgtgct ttacgtacca ggaagttgag
      901 cgcgcgctgg gtatccatca gacacaggcg cactttccag ccgatatctt ccagttccgc
      961 cagagtggag agcgcctcgt cgtactcatc gccctgatgc ccttccataa tcacccggct
     1021 caattgctcc aggtcgctat agatattttc aatttcatct gccaactgtt cgattttggt
     1081 ttcgaacaga tccagcagca actcgtaggc gttaccgtct accatcgact ggctacgggc
     1141 acgcatacga tacagacgaa aagcgggcag ttcacgctca cgcagagtaa acagacgacc
     1201 atcacggatg gtaaatgcca cagtggagtt accggcgtga tcttccgcat cttcaaagaa
     1261 gaagaaggag tgaatatgca ggccgtcatc gtcttcaaag aaacgggccg atgcttcgat
     1321 gtcttccagt tccgggcggg ttgccaggct ctggccaagt tcagattgta cgcgcagtcg
     1381 ctcgtcgtcg tccggttcga caagatcaat ccatactgca tttacaaggg gttgtgactc
     1441 ttcgacttcc agccgggtca gtcggttatt ttccagttga aatgcgctca gcatgaccgg
     1501 gactcccaat gcttaaaata tcggacagtt cggtgggcac acagaaacaa attgggtttc
     1561 agaccattaa acagcctgac tcagcgcgac gggaaaaatt gaggtcgctg acaaccgcta
     1621 aggctatcag caaaaaggga tagccttagg agttgatcct ggatgacagg atagtgagcc
     1681 agtatctact gggtgtgtcc aaggcgaatg tcctcttaga gtgatcgtgc gcgcatgtta
     1741 cgccagcaaa attttgccgt caacacgcaa cagaacacca cagaacaata gttgaacttc
     1801 gcgctttttg gtactcgtca aggtcgctaa caggataaaa aaatcccaca tccaggacct
     1861 ataatggcgc atcggctact cattggtaaa ggaatgatca ccttgaatct caaacggatt
     1921 tttctcgccc tcaccttact tcccttgttt gctgttgccg ctgatgattg cgcactctca
     1981 gatccgacgc tgaccgtaca ggcgtatacc gttaatcctc aaacagagcg ggtgaaaatg
     2041 tactggcaaa aagccaatgg cgaagcgtgg ggaacgttac acgctctgct ggcggatatg
     2101 aatagtcagg gtcaggtgca gatggcgatg aacggcggca tctatgatga aaactatgcg
     2161 ccgctcggtt tgtacatcga aaacggtcag cagaaggtgg cgttaaatct cgcttcaggt
     2221 gaagggaatt tctttatccg tcctggcgga gtgttttatg tcgcgggaga taaagtcggc
     2281 atcgttcgtc tggatgcctt caaaaccagt aaagagattc agtttgcggt gcagtcaggg
     2341 ccaatgttga tggaaaacag tgtaattaat ccgcgtattc tccccaacgt cgcctcgcgc
     2401 aaaattcgta atggagtagg gattaataag caagggaacg ccgtgttttt acttagccag
     2461 caggcgacca acttttatga ttttgcctgc tatgccaaag cgaaactgga cgtcgagcag
     2521 ctcctttacc ttgatggcac gatttcacat atgtatgtga aaggcggtgc gattccctgg
     2581 caacgttatt ccttcgtcac catgatttcc gtcgaacaga aaagttagag tgtcagatac
     2641 tgaatatggt gctgatgccg gatgcggcgt aaacgcctta tccggcctac atgacattgc
     2701 aatttattga atctgcatga ttttgtaggc cgaataaggt gcgcagcacc acatccggca
     2761 acgttatact gtctccagcc gggcgtaagc cgccaccagc catttaatac cctggccctg
     2821 gaacgccacc tgcaaacggc tatgttcacc gctgccttcc atattgacga tagtgccttc
     2881 gccaaacttg gcgtggcgca cgcgctggcc gagtttgtaa ccgctgtcgt tctcagccat
     2941 cggtgtaccc atacgctgat ggctgaccgg gcggcttacc gtagcgcgca ggcgcacctc
     3001 ttccacacac tcttccggca actcgccgat aaagcgcgac gggcgatggt aaacttcttt
     3061 gccatacaga cggcgagttt ccgcgtaggt cagcgtcagt ttctgcatcg cgcgggttac
     3121 accaacgtag gccagacggc gttcttcttc cagacgtccg ccttcatcca gcgacatctg
     3181 gcttgggaac atgccctctt ccataccaac gataaacacc tgaggaaact ccagaccttt
     3241 cgccgagtgt agcgtcatca actgcaccgc atcctgccag gtatccgcct gcccttcacc
     3301 tgcttccagt gccgcatggg agaggaacgc ctgcagcggc attaaatctt cgtcttcttc
     3361 gttgtagctg aattggcgcg ttgccgtcac cagttcctct aagttttcga tacgcgtctg
     3421 acctttttcg cccttctcct gctcgtacat ggtacgcagg ccggagtctt taattacccg
     3481 gtcagtctgt acatgcagcg gcatatcggc agtttcctgc gctaaggcgt cgatcagttc
     3541 cataaaccgc tgtaaggcgc tggcagcacg tccggcgagg gctttttcct gcaacagttc
     3601 acgacatgcc tgccagagtg ttaactggcg atcgcgcgat gtctgacgta ccacgtccag
     3661 cgtccggtca ccaatacccc gcgttggcgt attcaccaca cgctcaaagg ccgcgtcgtc
     3721 gttgcggttg gcaatcaggc gcagatacga gagcgcatct ttgatttcct ggcgttcgaa
     3781 gaagcgcatc ccgccgtaaa tacggtacgg catactggcc tgcaataacg cctcttcgag
     3841 cacccgcgac tgggcgttgc tgcggtagag aatggcgcac tcggcaagcg ctccgccgtt
     3901 gtcctgccag gttttgatgc ggttaaccac aaaacgcgct tcatcgagtt cgttaaaagc
     3961 gcaatagagg gaaataggct caccgtccgc gccatcggtc cacagttttt tacccagacg
     4021 cccgttattg ttttcaatca gggcgttagc ggcgctcaga atattgctgg tagagcggta
     4081 gttttgctcc agacgaatag tttcagcacc ggggaaatca ttaaggaaac gctgaatatt
     4141 ctccacctgc gccccgcgcc agccgtagat tgactggtcg tcatcgccaa cgatcatcac
     4201 tttgccggta tcgcccgcca gcaggcggat ccacgcgtac tgaatgttgt tggtatcctg
     4261 gaattcgtcc accaggatgt tagtaaaacg ttcgcggtag tgttgcagga tatgcggctt
     4321 gttaagccac agttcgtggg cgcgcagcag cagctcggcg aagtccacca ggcccgcgcg
     4381 gtcacacgct tcctgatacg cctgatacac cttctgccag gtctgctcca ccggattacc
     4441 gtagctttga atatgatgcg gacgcaggcc ttcatctttc tggctgttga tgtaccacat
     4501 tgcctggcgc ggcggccact gcttctcgtc gaggctcatg gctttgatca gacgcttaag
     4561 caggcgtagc tggtcttcgc tgtcgaggat ctggaaatcc tgcggcaggt tggcgtccat
     4621 atggtgcgca cgcagcaaac ggtgcgccag cccgtggaaa gtgccgaccc acataccgcc
     4681 ctggctggtg cccatcagtt gcccgatacg atgacgcatc tccgccgccg ctttgttggt
     4741 aaacgtcacc gccataatcg agtatggcga gcagttttcc acgctcatca gccaggcgat
     4801 acgatgcacc agtacgcgcg tcttaccact gcccgcgccc gccagcacca gaaggttgct
     4861 gcgtggagcg gccaccgctt cgcgctgttt gtcattaagg ctgtcgagca ggtaagaaac
     4921 gtccattggc accgccgcgt aaaaataggt tgggcggaga agcgcaacgc cctccgccaa
     4981 aaagctgggt atatatacag atttgctgat tatatcagcg aggtcagaga tgccaaccgg
     5041 gaaatttcca gatgcggcag taaacggctg tcccaggttt gcatcagatc gccattttcc
     5101 ggtctgatcc aacaggcctg cattccgctg cgaattgccc cacccacgtc agtggtgaga
     5161 tcgtccccaa catgtaagat ctcgccgatc ggcacgttga gtttttccgc agccaaaaag
     5221 tacatatcgc tgaacggttt tgagcgcccg tgcgggccag cgcgcagcac aaactcaaaa
     5281 taatccccca aaccaaacag ttccggctgg gcgttaccgt tggtgatcgc caccagcggc
     5341 catttcttcg ccagctgttt taaggtgtcg tgagtttgct gcgggacgtc gattcggctg
     5401 cgccatttgg caaagttaat cattgctgcg tgtgcgcctg cactggcttc ttcggcactc
     5461 agcccggcgt cgagcatcgc ttgttcaatc gaacgaaaac gccaacgcgt cacgtcgtga
     5521 taaatctcgg gttccgcttc ccgtaccgcc tggcgcaggc gttgcagatc ttcattctgg
     5581 aagctgcgca gcgccggatg ataattttgc acaaaggtaa gcgcctctcg ctcggtgcgc
     5641 aaaatcaccg gacggttatc gtaaagggta tcatccaggt caaaggtgag cgccgagatg
     5701 cgccccaaag gccggtaaaa acgcattatt tcccccgttt ggcgcgtgga tgcgccgcat
     5761 cgtacaccga ggcaaggtgt tgaaaatcaa gatgagtata gatttgcgtg gtggagaggt
     5821 tggcatgacc cagtagctcc tgcacaccac gaagatcgcc gctcgactcc agcatatgcg
     5881 tagcgaacga gtgacgtaat ttatgtgggt gaacgtgatt attcagccct tgttttatgc
     5941 cccattcggc aaagcgtttc tgcacattac gcgcggagat acgcttgccc agtttcgaca
     6001 gaaaaagcgc gtcgtcttcg ctaccaaaca ggtcgcgcaa atcaagccag tgctcaatcc
     6061 acgccacagc gttgcgacca atcggcaggc ggcgctcttt gctgcctttc cccattaccc
     6121 acacttcacc agactccagg tcgaggtgtt tgatatccag ccccaccagc tcggaaagac
     6181 gcagacccgc gccgtacatc acttccagca ttgcacggtc gcgtacagcg aggggatcgt
     6241 tgatatcaat atccagcagc cgattcatat cgtcgacgtc gatgtttttc ggcagatgac
     6301 gcggcgcttt cggtgccgaa acacctttcg ccgggttagc tttgagttcg ttctggctga
     6361 ccagccagtc aaaaaagcta cgtagcgcag aaagccgtaa cgcgaggctt gctgctccca
     6421 gccctttacg gcgactgcgt acagcaaaat tgcgtaccat cgctgcatcg cattgttgcc
     6481 agctttgcag gccgttttcg ctggcaaaat tgatgatcgc ctcaagctga cgctggtagt
     6541 taagtagggt tatcgggcta agctgacgct ccacgctcag ataacgtagg tagcgttcta
     6601 catcggtgtg taaatcggtc atacgcgttc aatccaacgc tccagaagct ccggcaacat
     6661 cagcgcaatt tcatgaagta actgcgttcc ctgcccttgt tgatagtgac tggcatcgcg
     6721 actggtaaac agcacgacac ccaaatcagc atcgcttccc agcatcgaca tcgccaccga
     6781 tcccaccgct ttcgcttccg gtagcaccac cagcagctct ggtccgttaa gcggcccaag
     6841 atagtgctgt tcctgcccca aacgctgaat acgcagcggt tcgaaagact gacggcttaa
     6901 tgccagatga gtgtggttcg acggcgcacc taagcgccag cgatccggaa acaggcgcag
     6961 actcgcacct gccaggccga gatcgcgcgc ccagcggtga aagcgcatca gcatatcgtc
     7021 gagactgctg gcggcggtga gactgcgctg caggtagagt aggcgataaa acaggccttc
     7081 gttggcgata gcctgttcca tcaacagcgc catgttctct tccagaacat gaatatgatt
     7141 acgtgcgcgg gccatgtgcc actcgaccaa cgaaacggtg ccgcgtaccg gatgcggcac
     7201 acgtatcgct tctactgcgc gcgcattgcg gataaaaaac tcaggatttt taatcagata
     7261 atcgacaacc gcccggtcat caagctccgt gagtgtttcc tgcagttctt cccctggttg
     7321 cttcatagat gaataaatcc gtcgtagaca tgtaccgccg ggccagtcat atataacggg
     7381 tgacccggac ctttccaggc gatatcaaga cgaccgccgg ggagttccac gcgtacttct
     7441 tcggccagca aaccttgctg aatccctact gcaaccgccg cacacgcgcc gctgccgcag
     7501 gcctgggttt ctcctgcccc acgctcataa acgcgtaaac gaatatgctc gcgcttaacc
     7561 acttgcataa aaccgatatt ggctcgctcc ggaaaacgct cgtggctttc caaaacagga
     7621 ccaagcgttt ctaccgccgc ggtatcgaga tcatcgacct gaatcacgca atgcggattt
     7681 cccatcgaca ccacgccgca taagattgtc tgctcggcgg cgcgcataat ataggtcttt
     7741 tccgctttgt tagcgcgaaa cggcacagcg gaaggttcga agttgggttc gcccatattt
     7801 acgcggacca gatcatcatc ggtgacggtc agaaccatcc gcccgttggc ggtgctgacg
     7861 cggatatcgc gcttattggt cagccctttc aggcgcacaa aacgggcaaa gcagcgcgca
     7921 ccgttgccgc actgcgccac ttcactgcca tcagcattga aaatgcgata gtgaaaatcc
     7981 agttcaggat catacggcgg ctcaaccacc agcagctggt caaaccctac ccccaggtgc
     8041 cgatccgcca ggcgacgaat cagctccggt gaaaaaaaga cattctgcgt taccgcgtcg
     8101 acgaccataa aatcgttgcc aaggccatgc attttcgaga actgcatcat ttactccaat
     8161 cacgcgggta cagaaactga cttctagtaa ttcacctggg atggaccatc gccagtggcg
     8221 cgatcgtttt tatccggcac cgtggattgc gtttgcgtct ctaccggttt ggtcggcggc
     8281 ggtgcatttt tatctgcagg cgggaaatag agcggacctt tcagaccgca gcccgtcagg
     8341 ctgaagagag taagtaatac agtgagtgcc ttaaacacgt ttttcattat ggattgcctg
     8401 taaagttcat cgctttctgc tttctatcat cgcaggagaa ggcgtaaaag caagcgttta
     8461 gcgactcttt atgaagtctg aaaagtgagg aaatgctgcg agtataacgc ttactcgtga
     8521 ggatttatgt tctgctggca gtttcgtgat caacacctcg cgccaggatg gctttatgca
     8581 ctatcgcaat gtggtttgtt tgttggcttg ttcattgttc ttatcgtcgg catggggatg
     8641 tcggcttgat gcacctgaac ataatattta ccagaaacag ggaaaaggcg tggtgtacct
     8701 gcgcccttat aaagaaacaa atctttctct acctgacgtg aattacaaga gtttgcgtcg
     8761 gttacccaat ttattaatag acccgacaac actagacgaa tgggataaag aaccgcctca
     8821 gactgatttg acaaccgact acctctatga gggcgcacag gcctggtatc ctcattattc
     8881 ctggtatagc gatggtcgca atatcctcta cgcgggtgaa gttgtgcaaa accctcccgg
     8941 cacacctccc gtcgatgtcg cctcgttcaa ggcatggggt gattttgctg cagataaaca
     9001 cagtctctat tttgaaggca agcgcaccga tgacaatggt gggagaaata gcttagatat
     9061 caaaacgctg catcaggtgg aattccggcc tccctgggct cctgacttac tggggctgat
     9121 attacgtgac gctaatttct tgtatatcaa tgggcatcgc ctcgctgacc ccgagagttt
     9181 ccgtgtgctg gcgcagaagt cctgggatca gcgaggaaag ttttccacca cgtttaaccc
     9241 ctgcgttgcc gtaccattcg gcccctggga taccctggct cgtacacgga ccaaaatcct
     9301 gatcaacggt gaacagcttg ttgccgaccc ggacaccttt tccgtcgtgc gctggatgcc
     9361 cggctcactc ttgacctggc gtgataaaaa cgggctacag cgtaaagtcc tcgacaagga
     9421 aaatctggcg tgggatgagg atttaacaaa gcactgtctg gatttttctc tgctggaaaa
     9481 gaaagtgttc tggcgtcagg ggcctgactg taaacaggaa gaattaccgg gactcgatcc
     9541 ggaacagttt caccccatca gtgatgctgt cgcacagtat caggactcgc tttataccat
     9601 catcgaaaca gagtctggtg accgcaagct ggagatcgtg aaacttgatg atcccaatct
     9661 tattatcaac aaacgtttca acgccgggaa acgccacggc tatttactta cgcgtgccga
     9721 agggtggcca taccattccg gtttacacgt gtttgaatct gacggaccgc tgatcttact
     9781 ggataaccgt tctccggatg aacgcgaagc ccatcttaat gaccatccct ttttgcgcag
     9841 atggtatgcc cgcgataacc gctacgttta cagctttgat ggcgcgcagc tctggcgata
     9901 ccgcaccgct gatccgaaac aagttcgctt aatctggaag gaacaacatt cgggatatgg
     9961 ctacggggta aattacaaaa cgggatatct ggacggaaaa attaccgatg acggcgaatt
    10021 tattcctgcc ccgcgcaatg aggcgacaaa atgaagggtc aaataatcac agttgcactc
    10081 atcctgttgg ggcacacatt tctgacacct gctgtacagg ccatagggtt cgactattac
    10141 aatgatcacg gtgttatgtc ttacggtaaa ggttatgggg aggatgagaa aatcattgcc
    10201 caatttccga agatgaacag agccgatttg cgccttgtga caaatatatc cggggagcgg
    10261 gagttagtgg aaggctatat acccactgat gaaataagca taaaaaatga ggaataccac
    10321 tgggtgacag acggccgcgt tattctctgg cgtggcaaaa tcgttagcaa tccaccggga
    10381 acacccactg tcgatattgc cagctttcag gctatgggcc gcttcgcggt cgataaatat
    10441 agcctctatt ttgatggaca gcgcaccgaa agtaatagcg gcgcgtcacg tgttgatctg
    10501 gcgacactaa aagctatcga aggtaactct accacgctgg tggatagtaa aaatctctat
    10561 ttgtccggtc gacgtcaggg gagcagcaat aatgttactg tattagaaaa aagatggtgg
    10621 ggtattaatc cacgtcttat gagtgtaaac agaaatttgt attccaatga tctgcttatc
    10681 cgcagtgggc agaatattta tttaaatgcc gttcacctta cagcgaatgc agactcattt
    10741 gagataattc gctggatacc tcattcactg ctggtttttc gcgacaataa gggtctgcat
    10801 cgttatccct ttgggcaatt gtctggcaaa gcgatacccg tagatgatga cgtctctttt
    10861 gaagtagggg aaagtcgcgt tcgctggcgt aaacagctca cgcccgaccg tcagtggagc
    10921 aagtggatag acctaccagg tattgaacct gaacaatttc atctgattac tggcaatatt
    10981 gcgcaatata aagatcggtt gtatgtaaca aaattatcga catttggtga agaccagctt
    11041 gagataatcc cgctggatac gccagacctg gtcattgatc actcatttaa tagcggcaaa
    11101 cagcatgctt attttatccg ccaattacgg tcaaagagcg tgcaaattat tccagttaac
    11161 ggtccgctaa ctaaaaacga tcgcttcgct tatgacgatc gcaatgttta tacatggacc
    11221 gatacagagg taaggattac accctccccc tgcccggcga aaacacgcgt cagagaagag
    11281 aatgtgcgcg aacttcacaa cagagacatt attattccgt tgacggatga atcatgccgt
    11341 aacgcagccg cagaggtgca aactttgaag ccctgacccc atgccgctat actgccgcca
    11401 tcacttaaaa acaggataca acaatgaacg acagtgaatt tcatcgcctg gctgatcaac
    11461 tgtggctgac cattgaagag cgtctggacg actgggatgg cgacagcgat atcgactgcg
    11521 aaatcaacgg cggcgtgctg accattacct ttgagaatgg cagcaaaatc attatcaacc
    11581 gccaggaacc gctgcaccag gtatggctgg caaccaaaca aggcggctac cattttgatc
    11641 tgaaaggcga tgagtggatt tgcgatcgca gcggcgaaac cttctgggat ttgctggaac
    11701 aggcggcgac gcagcaggcg ggtgaaacag tcagtttccg ctaagattgc atgccggata
    11761 agcctcgctt tccggcacgt tcatcacgaa aaatactgct gtaatagcgg cgtatcgtga
    11821 tcctgattgg caggcggcat gttaccgata gattttgtgc ggaacggaat cacctgttca
    11881 cgaccatcaa ccttcacaat ctgatagaac tgcggcaggt tgaagttgat gaagcttgag
    11941 ccgtaggtaa aacggtcatg cgatgacgag tagaagcgac tgacgtcacg taccagctcc
    12001 tctttgctgc cttcgcagtg gtgatacacc tcaacccggt tgctttcgtc gagaatgtag
    12061 atattaaagc cattctcgtc ttgcgtttct tcgaaaaaga actggatgat cccttcgctg
    12121 gcaaagccgt ccaccaccgc cggtaatttg acgtgattgg tttcaacctg cactgacagg
    12181 ccgtgcagtt tgttatgcga aatcgcgcca taaaactcga tggcgttttc cagtttctgt
    12241 accgatacat tcaggcgttc gaagaacaac ccccaggttt gaccagaaac gcgcagcgcc
    12301 ttgaaacgcc cggtttcctg gcgggtgctg gaaagacgca attcaataca ctcagaaacc
    12361 agttgctgca cgcgagtacg aattaagccg cgcagatgct ggctataaca gaagacttcc
    12421 acgctatctg gcggtgcggc gtcctgatgc attttgccga gaatagtttt cagggcttcg
    12481 atcatcgatt gctcgccgtt gaagtgcagc gtacgcactt cgttccacga gttgcggtac
    12541 agcaggtcaa cgctacctac caggcaattt tgattctcgc caaagctgaa gacatccagc
    12601 ttacggaaat cgaaatgcac cacctgattg cggaacgccg ctgtcgggtc atattccagg
    12661 ttaacgataa tcgccagatg gcggatctca cacgggctgt agagcgcctt cggtgtcggt
    12721 gcaggtaagc gcagcgggaa atggtgcgac acgtcggcga ccatctcctg caacttaggc
    12781 aaatcgacaa tgccgttacc tttaatatac aaacgggtgc gcgaggtcag caggccatta
    12841 aaccatgccc acgccaccag cttattcagg taacggttat attccagcgg ctgatggctg
    12901 atgatcgact caatatttgg cgcgcggtta tacagatacc aacctgaacg gttagcacgg
    12961 cccggcggca cataaataaa ggtcagattc ggttccgaga gatcgggtga aatctgcggg
    13021 tttaccagcg ttactttacc tggcaaagct tcaaacgcgg catacagctt acgcgtcagc
    13081 acaccgatat cctgcggact ggcggagacg ctaaggttat tgcgacgcgc aaagcggatc
    13141 agattacggt agctctgcat catcgcgtcg agcagctcgt tgtgcgcctc acgcacctga
    13201 tcaatcttcc agttagcgcg gttatcgagc attgccagac gagcttcgtc ccaaccccac
    13261 tcgctcacta actggctcaa cactgcgcga cgccagccta cgcaggcgcg ttcacggctg
    13321 agtttttcgc acacttttaa atagaagcag cgacgtacta aatccagacg ggtaaaatct
    13381 tcaatcgccg tcaggtattc agtaacacgc tccagcatca tgcagtatgg atcgagacca
    13441 aacgatacaa tctcgccgtc gtgcaaacgc tgtttgatat ctttcgccag cagacgtggg
    13501 ttcgggtatt cccaggaata ggcttccagc agcagtgttt tcagcaccgc tttgtatggg
    13561 gaatcgatac tcttgtagag ctgccaaagg ctggcaccaa agtactcttc agcagaaagc
    13621 gagcttaagc caccaagatc cagccattca tttggcgtca gcacgccctg cgcgtaaagc
    13681 gtcatcacat agtcgtcgta atgctcttct tcgtcgcacg gcaccatatt ccacagaata
    13741 cgcttaccgg cgagacgcac ggcggtacga taaaattcgt caagcagcag tatatgctgg
    13801 gtggagccac aatcttcgcc ccccaggctg ccgctttcat tatgacggaa gcggttttca
    13861 tcaatcagga agaagctgac ttccacaccc agcgaggcgg cccagctttc cagcaggctg
    13921 catttacgtt gtagcaactg gcgctcttcg ctatcgagcc aggattgatg acagacccag
    13981 atatccaggt cagaggaaca actttgccct acggacgaag tgctgcccat ggtgtataca
    14041 ccagtaattg gaagctcacc tttcggcggg tcctgtactg acattccacg atacagttca
    14101 agctcgttca ggtagtggcg ttgagtttca tcaggcgtgt aaaggcaaat gcctttggga
    14161 acgttaccat caaggtaacc cggcattagc ggatggtgat agtgcaacaa tgtcggcagt
    14221 agactgtaga cctgttggaa tgcaggcccc atagcagcaa gcgcgcgatc cacacgcaat
    14281 tgatttatgg catccagtct ctgtttcaga gtctcaatat agaggtacaa gacgtatcgc
    14341 ctgatttgct acccgtcatg actgtgattc cgccaacatc aacggtaaca cgcggcattc
    14401 gggatatttc gtatgtcaaa ggtaaccgtt accacttttc gcgcctggtt tttttagttt
    14461 cacgacgaaa aaatggtcta aaacgtgatc aatttaacac cttgctgatt gaccgtaaag
    14521 aaagatgcgc tacatacaag tgtagcaccg tttattctct gtaaattcct tattacgacg
    14581 gcgtgaaacg cctgtcagga tccactgcca gacctcattt tacggtttgc gcaggcgtct
    14641 acgtttcacc acaacactga catcactctg gcaaggatgt taggatggac cacggatgat
    14701 aatgacggta acaagcatgt tagacaatgt tttaagaatt gccacacgcc aaagcccact
    14761 tgcactctgg caggcacact atgtcaaaga caagttgatg gcgagccatc cgggcctggt
    14821 cgttgaactg gtaccgatgg tgacgcgcgg cgatgtgatt cttgatacgc cgctggcgaa
    14881 agtaggcgga aaaggcttat ttgtaaaaga gctggaagtc gcgctcctcg aaaatcgcgc
    14941 cgatatcgcc gtacactcaa tgaaagatgt gccggttgaa ttcccgcaag gtctgggact
    15001 ggtcactatt tgtgagcgtg aagatcctcg cgatgccttt gtgtccaata actatgacag
    15061 tctggatgcg ttaccggcag gcagtatcgt cgggacgtcc agtttacgtc gccagtgcca
    15121 actggctgaa cgccgcccgg atctgattat ccgctccctg cgcggcaacg tcggcactcg
    15181 cctgagcaaa ctggataacg gcgaatacga tgccatcatt cttgccgtag ccggactaaa
    15241 acgtttaggt ctggagtcac gtattcgcgc cgcgttgcca cccgagattt ctcttccggc
    15301 ggtagggcaa ggtgcggtgg gtattgaatg ccgccttgat gatgcacgca ctcgcgagct
    15361 gcttgccgcg ctgaatcacc acgaaactgc actgcgcgtt accgcagaac gcgccatgaa
    15421 tacccgtctc gaaggcggat gtcaggtgcc aattggtagc tacgccgagc ttattgatgg
    15481 cgaaatctgg ctgcgtgcgc tggtcggcgc gccggacggt tcgcagatta ttcgcggtga
    15541 acgccgcggt gcgccgcaag atgccgaaca aatggggatt tcgctggcag aagagctact
    15601 gaataacggc gcgcgcgaga tcctcgctga agtctataac ggagacgccc cggcatgagt
    15661 atccttgtca cccgcccgtc tcccgctgga gaagagttag tgagccgtct gcgcacactg
    15721 gggcaggtgg cctggcattt tccgctgatt gagttttctc cgggtcgaca attaccgcaa
    15781 cttgctgatc aactggcagc gctgggggag agcgatctgt tgtttgccct ctcgcaacac
    15841 gcggttgctt ttgcccaatc acagctgcat cagcaagatc gtaaatggcc ccaactacct
    15901 acttatttcg ccattggacg caccaccgca ctggcgctac ataccgtaag cggacagaag
    15961 attctctacc cacaggatcg ggaaatcagc gaagtcttgc tacaattacc tgaattacaa
    16021 aatattgcgg gcaaacgtgc gctgatatta cgtggcaatg gcggtcgtga gctaattggg
    16081 gataccctga cggcgcgcgg tgctgaggtc actttttgtg aatgttatca acgatgcgca
    16141 atccattacg atagtgcaga agaagcgatg cgctggcaat cccgcgaggt gacgacggtc
    16201 gttgttacca gcggtgaaat gttgcagcaa ctctggtcgc tgatcccaca atggtatcgt
    16261 gagcactggt tactacactg tcgactattg gtcgtcagtg agcgtttggc gaaactcgcc
    16321 cgggaactgg gctggcaaga cattaaggtc gccgataacg ctgacaacga tgcgctttta
    16381 cgggcattac aataactctc ataacaggaa gccataatga cggaacaaga aaaaacctcc
    16441 gccgtggttg aagagaccag ggaggccgtg gacaccacgt cacaacctgt cgcaacagaa
    16501 aaaaagagta agaacaatac cgcattgatt ctcagcgcgg tggctatcgc tattgctctg
    16561 gcggcaggcg tcggtttgta tggctggggt aaacaacagg ccgtcaatca gaccgccacc
    16621 agcgatgccc tggctaacca actgacggca ttgcaaaaag cccaggagag ccaaaaagcc
    16681 gagctggaag gcattattaa gcaacaagct gcacaactta agcaggcgaa tcgtcagcaa
    16741 gaaacgctgg caaaacagtt ggatgaagtc caacaaaagg tcgccaccat ttccggcagc
    16801 gatgctaaaa cctggctgct ggctcaggcc gattttctgg tgaaactcgc cggacggaag
    16861 ctgtggagcg atcaggacgt cacgaccgct gcagcgttgc tgaaaagtgc agacgccagc
    16921 ctggcggata tgaatgaccc gagtctgatt accgttcgtc gggcaattac cgatgatatc
    16981 gccagccttt ctgcagtatc gcaggtggat tatgacggca tcatccttaa gcttaatcag
    17041 ctttcaaatc aggtagataa cctgcgtctg gccgataatg acagcgatgg ttcgccgatg
    17101 gattccgacg gtgaagagct ttccagttcc atcagcgaat ggcgtatcaa tctgcaaaaa
    17161 agctggcaga actttatgga caacttcatt acgattcgcc gtcgtgatga caccgccgta
    17221 ccgctgttag cgccaaatca ggatatctat ctgcgcgaaa atattcgctc tcgcctgctg
    17281 gtcgcagcac aagctgtacc gcgtcaccag caagagactt atcgccaggc gctggagaac
    17341 gtctccacct gggtacgtgc ttactacgat actgatgatg ccaccaccaa agcgttcctc
    17401 gacgaggtgg accagttaag ccagcaaaat atctcgatgg atcttccgga aaccctgcaa
    17461 agccaggcga tgctggaaaa actgatgcag actcgcgtgc gtaacctgct ggcacaaccg
    17521 gcagcgggga caacggaagc taaacctgca cctgcaccgc aagctgcacc gcaagctgat
    17581 actccggcag ccgcgccgca aggagaataa ccatgctaaa agtgttattg ctctttgtgt
    17641 tgctgattgc ggggatcgtg gttggcccga tgattgccgg tcatcagggt tatgtgctga
    17701 tccagaccga caactacaat atcgaaacca gcgtcacggg cctggcgatc atattgattc
    17761 tggcgatggt agtgctgttt gccattgagt ggctactgcg gcggatcttc cgcactggcg
    17821 cgcacacccg tgggtggttt gtcgggcgta agcgtcgccg tgcacgtaag cagaccgaac
    17881 aggcgctgct gaaactggcg gaaggcgatt atcagcaagt tgaaaagctg atggcgaaaa
    17941 atgccgatca cgctgaacaa ccggtggtga actatctact ggctgccgaa gccgcgcaac
    18001 aacgtggtga tgaagcgcgc gccaaccaac atctggaacg cgcagcggag ctggccggca
    18061 atgacaccat tccggtagaa atcacccgtg tacgtctgca actggcccgt aatgaaaacc
    18121 atgctgcacg ccacggcgtg gataagctgc tggaagttac gccacgccat ccggaagtat
    18181 tacgtctggc ggaacaggcg tatatccgca caggtgcatg gagttcgctg ctggatatta
    18241 tcccatcaat ggcgaaagcc catgttggtg atgaagaaca tcgtgcaatg ctggaacaac
    18301 aggcatggat tggcctgatg gatcaggcgc gtgccgataa cggtagcgaa ggtttgcgta
    18361 actggtggaa aaaccaaagc cggaaaacgc gtcatcaggt agcgttgcag gtggcaatgg
    18421 cggaacatct tattgaatgt gacgatcatg atactgccca gcaaattatc atcgatggcc
    18481 tgaaacgcca gtacgacgat cgcctactgc tgccgattcc tcgactgaaa acaaacaatc
    18541 cggaacagct ggaaaaagtg ctgcgccagc aaatcaaaaa cgtcggcgat cgcccgctgt
    18601 tgtggagcac actgggccag tcactgatga agcacggaga atggcaggaa gcatcgctcg
    18661 ccttccgcgc agcgctgaaa caacgtccgg acgcctacga ttacgcatgg cttgccgacg
    18721 cgctggacag actgcacaag ccggaagaag ccgcagtgat gcgtcgcgac ggtttgatgt
    18781 taacgttgca gaataacccg ccacagtagt tccttctcac ccggaggcaa gcacctccgg
    18841 ggccttcctg acacataaaa aaacgcctgc tcttattacg gagcaggcgt taaaacaggt
    18901 ctgtatgaca acaagtgggt gcttcactca acgttgtgtc catggtgtct gatgaggcat
    18961 aagcgacatc tgtcagtgga cgataagcac cgtaaacggc tctgcgtcat tccggagttt
    19021 atgaggcact aaggcgaaca taagagatgg aatgagcatc tactcgttta ttatgccaca
    19081 gagaatcggg aaataacatc ccttaacact tgttatgaga taattctgta atcctcttgg
    19141 cttcctgagt aataacttcc tgagtgaata tttaacctga gcttgatcct acacatactt
    19201 attatgaatg ataaaattca ttcaattaat aacgcatata ttaattgccg ttaaaactaa
    19261 aaacagcatc aataatcaac gcgatataat aaacctgcct tacatatcaa ctgcgccaga
    19321 ggtaggattg aaaacgctct cctgattttt aaattcattt cttggataaa taaataattt
    19381 atttttatca ctattattta tgtaatcatc ctgtcaggga gagggatctc aattatcaat
    19441 gcttaattac gtcatcattt tgttgacatg aaaccggagc attttgcatg gaattttcgt
    19501 tttcacccaa acttcttgtt gttgctgtcg ccgccgctct tcctctcatg gccagcgcag
    19561 cagatacccc gtcaactgcc accgcacgca aaggctttgc cggatacgat cacccaaacc
    19621 agtatctggt taaaccggcg accactattg ccgacaacat gatgccagta atgcagcatc
    19681 cggcgcagga taaagaaacc cagcagaagc tggcagaact tgagaaaaaa accggtaaga
    19741 aaccgaatgt ggttgttttc ttgctggacg atgtgggctg gatggacgtc ggttttaacg
    19801 gtggcggcgt ggcggtgggt aaccctacac cagatatcga cgccgttgcc agccaggggc
    19861 tgattttaac ttcggcgtat tctcaaccaa gctcttcccc aacccgcgcc accattctca
    19921 ccggacaata ctccatccac cacggcattc tgatgccgcc aatgtacggg caaccgggcg
    19981 ggctgcaagg gttaaccacg ctgccgcagt tgctgcacga tcagggctac gtcactcagg
    20041 ccatcgggaa atggcatatg ggggaaaaca aagagtcgca gccgcagaac gttggctttg
    20101 atgatttccg tggctttaac tcggtgtctg atatgtacac cgaatggcgc gacgttcacg
    20161 taaacccgga agtggccctg agtccggacc gttctgaata catcaagcaa ttaccattta
    20221 gcaaagatga cgttcatgcg gtgcgcggcg gcgaacaaca ggccattgcc gacattacgc
    20281 cgaaatatat ggaagatctg gatcaacgct ggatggaata tggcgttaag ttcctcgaca
    20341 agatggcgaa gagtgataag cctttcttcc tctattacgg cactcgcggc tgtcacttcg
    20401 ataactaccc gaacgccaaa tatgcgggta gctctccggc acgcacctcg tatggcgact
    20461 gcatggtgga aatgaacgat gtgttcgcta atctgtataa agcactggag aaaaacggtc
    20521 agcttgataa cacgctgatt gtctttacct ccgataacgg accggaagcc gaagtaccgc
    20581 cgcacggacg cacaccgttc cgtggtgcga aaggttcgac ctgggaaggc ggcgttcgcg
    20641 ttccgacttt cgtgtactgg aaagggatga tccaaccgcg taaatctgac ggtattgtcg
    20701 atctggcaga tctcttccct accgcgctgg atctggcagg gcatcctggc gcgaaagtgg
    20761 cgaatttagt gccgaaaacc acctttatcg atggtgtgga ccagacatct ttcttcctgg
    20821 gaacaaatgg tcagtctaac cgtaaggccg agcactactt cctcaacggt aaactcgctg
    20881 ctgtgcgtat ggatgaattc aagtatcacg tcctgattca gcaaccttac gcttataccc
    20941 agagcggata tcagggtgga ttcaccggca cagtaatgca aacagccgga tcgtcggtgt
    21001 ttaacctgta caccgatccg caggagagcg actctatcgg cgtgcgccat attccgatgg
    21061 gtgtaccgct acagaccgaa atgcacgcgt atatggagat cctgaaaaaa tatccaccac
    21121 gcgcgcagat taaatctgat taagccggcg ctatcgctga gatctgcctt tgccggatgc
    21181 gatgctgacg catcttatcc agcctacaga acgctgcaat ttattgaatt tgcacgatca
    21241 tgtaggccgg ataaggcgtt tacgccgcat ccggcaatca accgcaggcg gcctccaaat
    21301 gaccgctttt ttacttactc accaccagca aatgcgcctg cataatgtcg ctggccgggc
    21361 gaccgtgcgc cagcaaatca gccatagctt taagatatgg cggtagatgg cggaaataac
    21421 gctgatatcc ggcacacaaa taattcagcc ccggtttacc gctagcatcg agcatgaagc
    21481 ggtgtttcgg gcagcctccc caacatgctt ttaacacgtt acaactgcga cactgcgccg
    21541 gtaactgctt aaatttatct tcaccaaaag cctgctgttg cggggaatcg atcatttctg
    21601 caattgtttg ctggtgcata ttccccagcc gatattgcgg ataaacatag tgatcgcagg
    21661 cgtaaacgtc gccgttgtgc tcaacaatca ccgagcgccc acaggttggc tgatgatggc
    21721 aaaccgcacc cggcgcaccg acaaaattgg caaacgccca ttcgatattc atcacaaaaa
    21781 tcttgccgac atcacgtttg atccagtggt cgaaaatcgc caccagaaat tcaccaaatt
    21841 catcggggcg caccgaccat tccgttagct caccctgaat atcaccaggc gcatgcaatt
    21901 tcagtccttc gcgggcagcc gtttcatcag ccaggcgctc gaccaccgga ataaactgga
    21961 tgaattccac tcccgcatcg cacaaaaaat catatacctg caacggttgc tgcgcgctgg
    22021 tgcgattaac gcagaccagc acgttatagt cgacatgatg tttttgcagc agcgtcaggg
    22081 cacgcatcac cagcttatgc gttggtctgc cacctttggt cacgcgatat tgattgtgga
    22141 tctcagccgg accatccagc gataacccaa caagaaaatg attttctgcc agaaatgcac
    22201 accattcgtc atcgagcagc acgccgttag tctggaagct gttacttatc ttcctgccag
    22261 caccatattt cgcctgtagc gctacggcac ggcggtaaaa ctccagcccg agtagcgttg
    22321 gttcgccgcc ctgccaggta aaagccactt cgttttgcgg ttcgctggca gcgatatagt
    22381 aacggatata cgcttccagc gtgtcatcgt ccatatgcgt gactggtttt tcgcggtaaa
    22441 gggattgttt ttcgagataa aaacagtagt cacagttcag attacaatcg gaaccgctcg
    22501 gtttcgccat cacatgaaaa gcacgcgttg gaacctgttg cagcatggtc gctccttgcc
    22561 tgaagtcatc cttcaatacg ttaagggcga aatcataccg tggggcaatg agttattacg
    22621 ggcgtttatt gcgaaggaag ggtgatagaa tgcacagaaa acaaaaaacc ccgccgaagc
    22681 ggggttcaaa attggtcggc gagagaggat tcgaacctcc gacccactgg tcccaaacca
    22741 gttgcgctac caagctgcgc tactcgccga aatactgctt tttgaatttt tagttcaatt
    22801 ctttaaagtc gtggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca
    22861 cctgaagcta gcgcgtctac caattccgcc accttcgcat tgtcacaact tctaataatg
    22921 gggtggctaa tgggattcga acccacgaca actggaatca caatccaggg ctctaccaac
    22981 tgagctatag ccaccactac aaatcttgtt acgcggtatt actaccaccg cagctcaagc
    23041 gccgggacta aatggcgcgc ccgacaggat tcgaacctga gacctctgcc tccggagggc
    23101 agcgctctat ccagctgagc tacgggcgct tagcgccgtt gcgggggtgg ataatacgga
    23161 cttcccaccc ctctgtctag tcccttttta aataaattgc gcgtttggtt acggtttgtg
    23221 cgttttgctg cttattcctc cagtttatgc gctttcccgt ggcgattaag gccaaaaact
    23281 ttataaatcg ccgtcaccgc cagcataaag atgatgccaa caaacagcga catacgcgta
    23341 tcttcattaa agtacatgcc gatcaaaacg caaatcagga atgccattgt tacgtagttg
    23401 gcccacggga acaggattga gcggaacgga tggctggcaa tcgccgcttt atgtgcacgc
    23461 cgaaaacgca gctggcttat caatatcaca aaccatggca ccatccccgg aagcacgctg
    23521 gcactgtaga cgtagacaaa cacacgctgc ggattgggaa tgatgtagtt caggcatgag
    23581 ccaattagca gaatagcaat agatactgcc acacccgcaa ctggtacgcc gtgacgggaa
    23641 actttcgcca tcgccgccgg taactgacgg tttttcgcca gtgcgtagag catacgtccg
    23701 caactgtaca tgccgctgtt acagccagag agcgcagccg tcagcaccac aaagttgata
    23761 atgcccgccg ctgcggtaat accgattttg gcaaaagtca gtacgaacgg gctgccgttg
    23821 ctgccaattt cattccacgg gaagatggtg acgataacga aaatcgcgcc tacgtagaaa
    23881 atcaggatcc gccacagcac cttgcctacg gcactgcgca gcgtcacctg cggattcttc
    23941 gcttcaccgg cagtaatgcc aatcagctcc acgccctggt aggacgccac cacaatacac
    24001 agcgcggtca ggaacccttt ccagccaccc gcaaagaaac cgccatgctc tgtgagattg
    24061 ctaaaaccaa tcgactgccc accattgcca aagccaaaga aaatcacgcc caggccaatg
    24121 acaatcatca cgataatcgt ggtgactttg atcatcgcga accagaactc gatttcgccg
    24181 tacaaccgca ccgccgccag attcgccaac gccaccagcg ccaccgcgat caatgcgggt
    24241 atccactgcg ccatctccgg gaaccagaac tggacataaa cgccaatggc ggtaatttca
    24301 gagatcccca ccgccatcca cataaaccag taagaccagg cggtgagata accaaaaaac
    24361 gggctcatat aacgatgcgc ataaacggcg aacgaaccgg tcaccggttc gaggaacaac
    24421 atttcgccca ttgaacgcat gatgaaaaag acgaacagcc cggcgatgat ataggccaac
    24481 aatacggatg gcccggccca tttcaaggta ctggcggccc ccataaacag gccgacgcca
    24541 atggtgcccc ccagggcgat gagttcgata tgtcgagctt ccagcccacg ctgtagctct
    24601 ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat gctttccggt gttaccggtt
    24661 atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa ttcaggagaa atggcaaata
    24721 aagcattaaa aatttgaatg ctttgtgtaa taaaaagaca ggcaggcgac gaagtgacca
    24781 ctccgtcgct ttacaaagag aggaaaatca gagctcgccg gtgtaatgcc agcgtaaata
    24841 acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc gaaagcaggc ggtagagcca
    24901 ctccagcccc agcgtttgcc agattttcgg tgctcgtttc acgtgaccgg tgaaaacatc
    24961 gtaagtcccg ccaacgccca tatacagcgc atctggatgc accagacggc agtcgcgcat
    25021 gaagatctcc tgctttggcg atcccatcgc aacggtgacg atttgcgctc cgctggcatg
    25081 aatgcgttca aacagcgcct gacgctgctc gggtttaaaa taaccatcct gactgccaac
    25141 gatattcaca ttccactggt tgcgcagttt agcttcagtt tgcgccagca cttcaggttt
    25201 accgcccaca agaaatactg gcgtcccttc tttgcctgcg cgcgccatca gctcttccca
    25261 gagatcggca ccggcaacgc gggaaacctg cgcctgcggg tactttttac gtactgaacg
    25321 tacaacgctg atgccatccg catatttaaa ttcggcagcg ttaattaact ccctgacctc
    25381 ggcgttatct tcaatggtca gcattttttc agcattaatg gcaaccagcg ttccctgctt
    25441 aagctgcccg tcagcaaaca gataatcgag ggcgtgctgc atatcacgcc aaccaatcaa
    25501 ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg ttattcattg ttatccttca
    25561 acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga gtccggcgct ttcaaaaagc
    25621 cagtacaaca gttttgcgat catcagacat gcgccgaaga ccacgataaa aaagactacg
    25681 cgtgagacaa acgaatccag cccttcacgt gccagcacga tcatattgaa gatcgcccca
    25741 aagcagaaac tgtgcaatat cgcagcctta tagcgattag tctcgcggtt gcccagctca
    25801 tacagccagt cgaaccattt gatgatcagt ccaaccacaa tcgccccgag cgggatgaac
    25861 aacgcgccgc ccatcaccac cagtgagcct ataagcgtag gcgagatcgc cagtccggag
    25921 tggttattca gcacttccca ggtaaagtag ttggctgagt tcagcaccat actcgggcga
    25981 cccggccaca gccaggaagg gataaagaca tagaaatcgc ggacaattgg agccaggccc
    26041 tggaagtcga tgttgtcgta gttctgcaac agcaacgcca gattctccca cggtgaaaag
    26101 gtgtcgcgag tgagatagag aaacgtatag aacgcttcat cgccgctcac attcattcca
    26161 tagcgtttta acgccagcca gaacatgccc acaatcccca gcacgcccgc caccgccagc
    26221 atccacaacg aaatccagcc gcgaataatg ccaataaaca ggaagatagc gaatgcgatg
    26281 atgatattgg cgcgagtgcc gccgacaatc atataagtca gcaagccaaa agcgaccgtg
    26341 ctgacgagga aaaacagcca cgctttgctg tcctggcgca gaaaatagac caccagcatc
    26401 gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc cggagacttc actggaaaag
    26461 atctgactgt aggagttaag ccggaacagc aaaaagccgt tgtgcatgaa gaagatgccg
    26521 acgcttacca gcgcgatacc catcaggatc acccacgtaa gattggtctc cacgcggttc
    26581 atggtaaaca gcggacggcg cggtacatca gcaacgcgtt tgcgtaggcg ggttttgtag
    26641 gtgacatagt aaaccgcgta gaagcagcct gcagaaagca acgcctgcaa caagatttct
    26701 ggcggcgcga caccaacatc aaagcgaaat accagcacgc tggtcagcgg gaagccgaag
    26761 aaaaaggtga gcaaaaacag caatgaaaag aagacattga agttaaagcg cacacggcga
    26821 aactcaaacc aggtcagcgt ggcaataaac agcgtgcaga gcagccagac cacaaacagg
    26881 ccactgaatt gcagcagact catgcgacct ccccggcggc aatcgccaac gcccgctgcc
    26941 agccttgtag atagttaggg ctaaagaagg caatggtgtt tttatccacc gacgccaact
    27001 ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc ggtagtaaac agcaccggta
    27061 aatgctgttc cgtcatatcc tgccagaacg gattttcccg gttaagcaca caaggaatgc
    27121 ccgcctgaat cagtaagcac agcgtaccaa tgccctgctg gcgggcaaaa ataaagtaac
    27181 caagatcgca ctgacgaagt agcgccagat aggcgtcaaa ttccagtttt tcgctcagaa
    27241 ctcgcagatt ttcttcgctg aataacttca gccccgcctg acgaacttcc tcgatgtacg
    27301 cttcgttatt aggcggatat cccatcggca ccaccacttt taccgtatcg ccaaattgct
    27361 gatgaacggc gcgcaaggca gcaatatgtt cattgctgcg gtcaccggag ttccccacca
    27421 gaacggtcat tttcccttca cgttgccgat cgttcgccat cgtattgaga gaagggtcca
    27481 tccgcgtcgg gaagtacagc agttcgcccc gcacctttgg atgcgttttg gcaaaaaagc
    27541 tcaaatcacc gcgggtggca aatacacagc cgactcgctt ttgcgccagg cgacgtagcg
    27601 ggtaaaaaag cttatatctc aagccactgg aaagctcata caggtctgcc ccccagatat
    27661 gccagaaaaa ctggctgggc ttaatgccac cactcagcag agccagccac agtgtgggat
    27721 tgaactgacc gtggaaaaaa aatcgctgct gacggttagc ttttgctttc gcgatgaccg
    27781 cttccgccag cgattttttc ccagggaaaa attgcacaga aagcgccgga cagctatcac
    27841 ttaagccgtc gtccttgcca acaaccataa actcgcgcgc gtgctcgctc gtcgcggcca
    27901 gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg agggatatcc gatcccagta
    27961 cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa aacacgccac aacaaagaga
    28021 aaaatagacg atataggttg ccatataggc ctgcgccgcg cccagtgcac catgcgcagg
    28081 gattagccag tgggcaaata ccatcaataa agtgaactgg ctgacttccg ccagaatata
    28141 aaaccgcagt gaagctttgg cgatcaccag ataaccaaag acataagcgc ccacttttaa
    28201 cacatcaccc actaactgcc aggcaaagag atcgcgcata gcggtaaatt tattcgacaa
    28261 cagcagccag atagcaaaat cacgcagcaa ccagacggta aaactcgccg ccgccactgc
    28321 cggtaagaca aatttcagcg atttaaccac ttcccgggtg atatcgcgct tttccgttag
    28381 ccgcgacaac gtgggcagca aatatacgct gaacgatgcc gtaataaatt gcaggtaggc
    28441 atcggaaata ctgctcaccc cttgccagat ccccacctca tcccagctat actgcgccgc
    28501 cagcagttta cgcatcatga tgtaagcaac aggcaaggtc accgacgtaa tcaacgccat
    28561 gagcgtaaat ttgctcaact gccctgccag accgttatcc cagctgggtt tcagatagct
    28621 taacgggatg acaccacgtt tgatcaacat gatggcggca gggattacca ccagcgcggg
    28681 aatcagcgcc agacccagca acgccccttc ataaccgccc aaacggtaac tgacgtagta
    28741 cgcgagaacg ccaatcaagc tgccgacaat cagcgataac gcattacctg cggcatcgcg
    28801 aaagcctttc atcaacgcca gtaacaggtt gccccaggcg atccccattt gcaccaacgc
    28861 cactaaacgt accagcccct gatagtcggt attaccaaac aatccctggc tgattggcgc
    28921 tgctgccagc acaaaaacca gcgccatcag cgtagagaaa ccaagtacca tcgctgatga
    28981 agtgccgacc acgcggcgca gctgttgcgg attatcatgg tactgggcaa cgtatttggt
    29041 tacaccgtta aagatgccag ccccggcaag cacgccgagc acggtaatca actggcggaa
    29101 atttgccgcc agcccaagcc ccgccggacc aaatgacacc gccagcaact taccgaccag
    29161 taacccggca ccaatcttga ccagtgtact ggccgccgtc cacaaggacg cttttgccaa
    29221 cgacatatca ggagaagtag ttcaacaaag tcgcaattac cgtacgctga ttgacgggcg
    29281 acaggttgta gaacagcggc aggcgcagca ggcgctcgct ctctttggtg gtgtagctat
    29341 cttcaccgtg gaactcacca aagcgttccc ccgcagggca accgtgcagc ggaatgtaat
    29401 ggaacaccgc catgatttcc gcttctttca gaaagttaat caacgcgctc cggtcatcaa
    29461 tatcccgcag tttaatgtag aacatatgcg cgttctgcac acagccatcg ggaatcgacg
    29521 gcagctcgat acgcccggct ttcgccagag gtgctaacgc atcgtagtag ttttgccaca
    29581 gcgccagacg ttgctggttg atacgatccg ctgcttccag ttgcgcccac aggtacgcag
    29641 cttgcagatc ggacatcaaa tagctggagc caatatcgcg ccaggtatat ttatcgacct
    29701 gaccacggaa gaactggctg cggttagtgc ctttttcacg gatgatctcg gctcgttcga
    29761 ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc acccgccgtg tagtttttgg
    29821 tttcatggaa gctaaagcag ccaatatgac caatggttcc aagcgcacgc cctttgtaag
    29881 tggacatcac gccctgagcg gcatcttcta ccacaaacag attatacttt ttcgccaacg
    29941 ccataatggt gtccatttcg caggccacac ccgcgtaatg gaccggcacg ataacgcgcg
    30001 ttttgtcggt gatcgccgct tcaatcagcg tttcgtcgat gttcatggtg tccgggcgaa
    30061 catccacaaa gacgattttc gcgccacgca gcacaaaggc attggcggtg gagacaaagg
    30121 tgtagctcgg catgatcact tcatcgccag gctggatatc gagcagcagc gccgccatct
    30181 ccagcgaagc ggtgcaggac ggcgtcagta acactttggc gctgccaaaa cgttgctcca
    30241 gccactgctg gcagcgacgg gtaaaaccgc catcgccaca cagtttgccg ctacccattg
    30301 ccgactgcat atagtcgagt tcggttccca ccaccggcgg tgcgttaaat ggaatcatgt
    30361 gatcacctgt ataaccagta cgcggtgctt tctacattcg caccactttg tatgtatcgt
    30421 ttaagcgcgg cggtgttgcc catttgggtc gccacccgca aagttgtttt accgcgagca
    30481 tacgcccagt ttagcgccgt ttgcatcagc tcagcacctg caccgcgtcc agccagcagg
    30541 cctattcgcg catctgtcgc attgagttcc cgtaaagaga catagccgcg aatatcgccg
    30601 gacgccgcac gtaaaatcag acattgatga tcaaaggtgc cgcgcacggc attttcaatc
    30661 cactgtgcat aaaagcgact gctggcgtca ggcgcatacc acggcgcacg aaaacggctt
    30721 tgcgcaaatg cggcgctggc taactgacgt aatgcgggaa tatcggtctc ttgtgccact
    30781 acagcaccgc tatcactggc attgttcacg ggtagcgcca aatcaacttc accttctacc
    30841 agggagaatc ccagctgttg cagggcatcc agttcacccg tatttgatgc cgcaattttg
    30901 gcctgcaccc gtgaccacgg cgctaacgcg tctggcgtca ggagcggtgc ttcagacgta
    30961 atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt cccaggttag tggctcaata
    31021 ctggcgcgga cgggcacgaa gtaactccag cagatattgg ccgtagccag ttttcgctaa
    31081 tgaactggca gcacgcttca caccctcgtc atcgagccag ccgttacgcc aggcgatctc
    31141 ttccaggcag gcaatcttaa agccctggcg tttttccacc gtctgtacaa aggtgctggc
    31201 ttcaatcagg ctgtcgtgag tgccagtgtc cagccaggca aatccgcgcc cgagcagttc
    31261 aacggtcagg ttgcccgcct cgaggtacat ctggttgatg gaggtaatct ccagttcacc
    31321 acgctccgac ggcttcacct gctttgcgta ctccacgact ttactgtcgt agaaataaag
    31381 cccggtcacc gcccagtttg acttcggctg ttttggcttt tcttccagcg agatagcgcg
    31441 gaaattgtcg tcaaactcca ccacgccaaa gcgttccggg tccatcacct gatagccaaa
    31501 aaccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc ttcggactga agccctgacc
    31561 gaagaagatg ttatcgccca gcaccaaaca agaaggttca ccattaagga aggtttcacc
    31621 gatgataaag gcctgcgcca gaccgtccgg gctgggctgt tcggcatatt ccagctggat
    31681 accgaactca ctaccatcgc ccagcaggcg ctggaaataa cctttatctt ccggcgtagt
    31741 gatgatgaga atttcgcgga taccggccag catcagcacc gacagcggat agtaaatcat
    31801 tggcttatcg taaatcggca acagttgctt cgatacgccg cgcgtaatcg gatgcaatcg
    31861 ggtaccggaa ccgcccgcca ggataatacc tttcatgttt gcctccgctg aaaattagcc
    31921 tttcagacct aaacgctcgc cctgatagct gccgtcctgc acctgcttcc accagctttc
    31981 attagccaga taccactgaa ccgttttacg cattccactt tcaaaggttt cctgcggcag
    32041 ccagccaagt tcacgggcaa ttttcgaagc atcaatggca taacgcagat catgccccgg
    32101 acggtcagcg acaaaggtga tcaagtcacg atagtgcgcc acgccgtgcg gcttgttcgg
    32161 agccagttct tccagcagct cgcaaatggt ttccacaaca tcgagattct tacgctcgtt
    32221 gtgaccacca atattatagg tttcaccgac tttcccggtg gtcgccacgc aatacagcgc
    32281 gcgggcgtga tcttccacat acagccagtc acggatttgc tgcccgttgc catataccgg
    32341 cagcgattta cccgccagcg cgttgaggat catcagcggg atcagttttt ccggaaagtg
    32401 gtaagggccg tagttattcg agcagttggt gataagcgtc ggcagaccat aggtccgcag
    32461 ccaggcgcgc accagatggt cgctgctggc ttttgacgcg gaataagggc tgctcggcgc
    32521 atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc aggtcaccat atacttcgtc
    32581 agtggagata tgatgaaaac ggaacgctga ttttttatct tccgttagcg cattccagta
    32641 agcccgcgcc gcttcaagca atgtataagt cccgacaatg ttggtttcaa taaacgctgc
    32701 cgggccgtca atagaacggt caacatggct ttcggctgcc agatgcatga cacagtctgg
    32761 ctgatgctca gtgaatacgc gcgccagttc tgcccgatcg cagatatcaa ctttctcaaa
    32821 ggcaaagcgc tcgctttgcg cgaccggtgc cagcgacatc aggtttccgg cgtaggtcag
    32881 cttatcgacc actaccaccg cgtcgctcgt ttcgttgatg atataacgca ccagcgccga
    32941 gccaataaac ccggcaccac ctgttatcag aatttttctc atcagcgcca gactcctttg
    33001 gcatcgacga catactgctg atggacattg tcgccattga taactttgaa ctgactatga
    33061 tcgaccagca tcaccagcac gtctgccgtt gccagcgcct cgtcaagctg cgccagagta
    33121 caaagcccgg tcagtttttt cggcaactgg tggatgttag gctcaacaac cagagtttcg
    33181 ccgctatgcc actgggcgat cagttcagcg atttccatcg ccgggctttc gcgcaggtca
    33241 tcaatattcg gtttaaacgc cagaccaaag caggcgattt tcagttcact ggcgcgttta
    33301 tcggtagccg ccaggcaatc agccaccgcc gctttcacct gatcgataac ccagaacggt
    33361 ttgtgatcgt tcacttcgcg cgcggtacgg ataagccgcg cctgctgggg gttctgtgcc
    33421 acgataaacc acggatcaac agcaatgcag tgaccgccca cgccagggcc aggctgaaga
    33481 atattaacgc gagggtgacg attcgccagg cgaatcagtt cccagacgtt aatcccctga
    33541 tcggcacaaa tcagcgacaa ttcattagca aaagcgatat tcacatcgcg gaagctgttt
    33601 tcggtgagct tacacatttc cgccgtccgc gagttagtga cgacacactc accttcgagg
    33661 aaaattttgt acagttcgct ggcgcgggcc gaacaaaccg gcgtcatacc accaatcacg
    33721 cgatcgtttt taatcagctc gaccataacc tggcccggta acacgcgttc cgggcagtaa
    33781 gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga aagtgagatc cggacgcatc
    33841 tctgctaacc attctgccat cttctcggtt gaccccaccg gcgaggtgga ttcaaggatc
    33901 accagcgcgc cttttttcag cactggcgca atggagcgag cagccgattc aacgtaggtc
    33961 atatctggct catgatcgcc tttaaacggc gtgggtacag caatcagcca ggcatccgct
    34021 tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta cggcagtttt tactacgctc
    34081 gccaaatcag gctcgacgat atggatttcg ccacgattga tggtatcaac cgcgtgttgg
    34141 ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa acgctgctgc tgtaggcagc
    34201 ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac tcatagtgat atccgattat
    34261 tttttaacgc ttccagaatg cgagagcatg cctgaccatc accatacggg ttatgggcgc
    34321 ggctcatagt ttgatattcg ttttcgtctt ttaaaagacg cgtcacttcc tcgacaattc
    34381 gctgcttatc cgtgcccacc agacgcaccg tacccgccgt caccgcttcc ggacgctcag
    34441 tggtatcgcg catcaccagc accggtttcc ccagcgaagg cgcttcttcc tgaatgccgc
    34501 ctgagtcggt caaaatcagc caggcgtggt tcatcagcca gacaaacggt aaatactcct
    34561 ggggatcgat cagaatgaca tttttcacat gccccagaat gcgattgacc ggctctctga
    34621 cgttcgggtt gagatgcacc ggatagacaa tctggatgtc ctggtgcgtg gtggcgatgt
    34681 ctgccagcgc gtggcagatt tcttcaaagc cacgaccgaa actctcacgc ctgtgaccgg
    34741 tcaccagaat catcttttta tcggggtcga taaacgggta atttgccgcc agttctgaac
    34801 gcagcgtgtc gctgctcatc acctgatcac gcacccataa cagtgcatca atgactgtat
    34861 taccggtaat gaagattcgg ctatccgcaa cgttttcacg cagcaagttt tgccgggaag
    34921 tttcggttgg agagaagtga tacatcgcca gatgcccggt caatgtacgg ttagcctctt
    34981 ccggccacgg cgaatagaga tcgcccgtgc gcagaccagc ctcaacgtga ccaacaggaa
    35041 tacgctgata aaacgccgcc aggctggttg ccagcgtcgt cgtcgtatcg ccgtgaacca
    35101 gcacgacgtc tggtttgaac tcggcaagaa taggttttag cccttccaga atccgacagg
    35161 ttatctctgt caggccctgt cctggctgca ttatgttgag atcgtagtca ggtacaatgg
    35221 aaaagagttt cagcacctga tcgagcatct cccgatgctg cgcagtgacg caaactttag
    35281 cctcaaaaaa aggatctttt gccaacgcat gcaccaacgg agccatcttg atggcttccg
    35341 ggcgcgtacc aaatacagtc agtactttca catcgattct cttcgaataa gcggcgagca
    35401 cctttgcgct caccgcagca gtgttgctat ttcgagcaac ggcgggttaa tgcgacacca
    35461 gccccgatca gccccccgac aatgccccac ataatcatca ggaaggcacg acgtgggcta
    35521 tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat aggtctgaaa acgcggatcc
    35581 agggttggac caacattcag ggtgtttaac atggcccgat tctgatcata gtcgagatca
    35641 aaggccggac cgacggcctg taaattttcc agtcgagcct ggagcattgg acgcccaagc
    35701 aggaacattt ctgaatcagg taattcctcg gcaggcacat ctgtcgcact gcgcgaaata
    35761 ttatgctgct cagcaatttt cagcgcctgc tcaatgctgt tcatccggcg gtcgtagatg
    35821 gctttcgcca cctcttcctg acgcttcacc tgagctttca tctggatggt gcgtgccgcc
    35881 catgcgcctt tcagctcatc attcagatgg ctggctgcac gctggctggc aaaagcaaca
    35941 tactgacgta acaggttatt agcgtcaggc gcggtttcgg caataagctt cacgctgtca
    36001 ttgaccgcgc gggtaaagtc tccggggata aactggatgt tgttaatcat ttcatccagc
    36061 aacgccgcat cggctttgct gttgcccacc atccgctgct tgtaatagtc ggtttgcagc
    36121 cagaactctc tgcgggtatc ccacgaggcc agttgcataa caaactcttt ataggcttcg
    36181 tccatgaccg atggttggtc ggcagaagcc atgtttgaac ggacatccag gttacgcaaa
    36241 aattgctgct gcgagtaata tccccccagc atattcaccg ttggacgatc ggtaatcgcc
    36301 gtcgagctcc actcctgacg agcaaaaaac gtatacgcca gcgcgattaa cgcaaacgcc
    36361 agccccatgc caataatcca tagcttccca gcccacaagg tacgaaacaa cccacgaata
    36421 tccagttcat tttcagcgtc ttcggccggt ttcccaggca ttggttgtgt catcacatcc
    36481 tcatttattt ggttaaattg gggctgccac cacgatttct acgcagtctg cgttttacgc
    36541 gcttaataaa gcgagcaact ttccaggcac gcttaatgca atatccatag aggaagaatg
    36601 ctagcaaaaa gagcaccagc atgacccact ccgggacaaa atgagaatat tctgccagca
    36661 cgccaatgga agcgagcagt gctgcggcaa gggtaatcag cacaaacgcc tggcgggaag
    36721 taaacccggc acgcatgatc aaatggtgaa tatgctgacg gtcaggagag aatgggctca
    36781 tgcctttacg caggcgacgg tacataatcg ccaccatatc cattagcgga atggcgatta
    36841 tccacaaagc ggtaaccggg ctgatgggat gggttttgcc ctgggtcgtt tcgagcagga
    36901 tccagatcac ggtaaaacca atcagcgtac tgcccgcatc acccataaag actttgtagc
    36961 ggcgacccag gataccaagg ttaagcatga tgtatggcag gatggcggcg atcatcgcaa
    37021 agcaccagat tgcgaggctg gtttgcccgt cgaaccacaa aatcataccg attgctgcaa
    37081 acgagacgca ggacaacccg cccagcaagc catcaatgcc atcaaccatg ttgaacgcat
    37141 taatggccgc ccagacggca aatagcgtca ggaagtaacc aaacggtccg agcaccatct
    37201 cccaggagcc aaagatataa cccaggctac tgagataaag cttgccgaac accatcataa
    37261 caatgccaac agcggcctgt atggtggcac ggatttttac gctgatatca aaacggtcat
    37321 ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag atagagagat gcatgcggaa
    37381 tatagtaatc gacaattccg aacgtgaagc aaatccctgc gtaaaccgaa atccccccaa
    37441 cgagaggtat caatccctgg tgacgtttgc ggaagtttgg tttatccact aaaccgactt
    37501 tttttgccac cttacgggca aaaaacagaa acagtgtcgt gaataaaaaa atactgatga
    37561 gatcagtact cactgtcagt aaattcacaa tgcatgctct cagagaaaat tattagcaga
    37621 agtataacca cgaagacctt tattcagaag ggaaagtccg ttccgaaccc accagtcctg
    37681 tgcaaaaatt aaccatttga tatgcaaatt gcataacatg ccagcaaatt ccaggaattt
    37741 tcctaacatc gccagcgcgg cattaagatt acagatataa aagcaaaacg ccacgtaaac
    37801 acgtggcgtt tttggcataa gacaaattta tgagcgtttc atcatttcga agaaatcgtc
    37861 attggtcttg gtcattgcca gtttattaat gaggaattcc attgcatcga tttcgcccat
    37921 cgggtgaatg attttgcgca ggatccacat tttctgcagt tcttcctgag tcgtgagcag
    37981 ctcttcttta cgggtaccgg aacggttgta gtcgatagcc gggaagacgc gtttttcagc
    38041 gatcttacga gagaggtgca gttccatgtt gcctgtacct ttaaactctt cgtaaataac
    38101 ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg ataatggtca ggctgccgcc
    38161 ctcttccacg ttacgcgccg caccaaagaa gcgtttcgga cgatgaaggg cattggcgtc
    38221 cacaccaccg gtcaacactt tacctgacgc cggaacaacg gtgttgtaag cgcgcgccag
    38281 acgagtgatg gagtcgagca gaatgataac gtctttcttg tgctcaacca ggcgtttcgc
    38341 cttctcgatc accatttccg caacctgaac gtggcgagat gcgggttcgt caaaggtaga
    38401 agcaacaact tcacctttta ccagtcgctg catctcggtt acttcttccg gacgttcgtc
    38461 gatcagcaga accatcagca cacaatccgg gtggttgtaa gcaatgctct gagcaatgtt
    38521 ctgcagcagc atggttttac cggctttcgg cggtgccaca atcagaccac gctgaccacg
    38581 accgataggt gatgccagat ccagtacgcg agcggttaaa tcttcagtag aaccgttacc
    38641 acgttccata cgcagacgag agtttgcgtg cagcggggtt aagttctcaa agaggatttt
    38701 gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta actttcagca gcgcaaaata
    38761 gcgttcacct tctttcggcg ggcgaatctt accagagatg gtatcaccag tgcggaggtt
    38821 gaaacggcgg atttggctag gggaaacgta gatgtcatca ggaccggcga ggtaggagct
    38881 gtctgcggaa cggaggaaac caaatccatc ctgcaatatc tccagtacgc catcaccaaa
    38941 gatatcttcg ccactctttg cgtgctgctt caggatggca aaaataatgt cctgcttacg
    39001 catacgagcc aggttttcca gccccatatt ttcgccgaga gtgatcagct cagaaaccgg
    39061 cgtattcttt aattcggtaa gattcatagt ggtgtgagtt cttaaacttg gggtaaatct
    39121 cgaacttaat gttgtgaatg gtatggcagg atcatccatg cctgtttacg gtcatcgact
    39181 catgtctttt cgctgcctgg tcacaggaga gtacgcagaa ctgaaacgac aagacggatt
    39241 gagtgacgag ccagaaatct gttcacttcg catttaagat aaaacgggaa gcgttggaaa
    39301 ttacaagatt caaacttaat aaggtatgtt taatacgaag tcaacactaa gttagcatga
    39361 ctcacgccgg gcgtccagtt tttagcgacg gggcacccga acatgaaatt cccttacgcc
    39421 aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcgcccac tttggttgcc
    39481 gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc atatttcggc
    39541 gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc ctgatattcg
    39601 tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca ctctgcccag
    39661 aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact gtcgtcagtc
    39721 aggtgaataa ttttatcgct catatataac tccacaggaa taagcctggc gtgttggttt
    39781 cgttgttggt gtaacattaa ccaactaaag gttgacttta tttcaccgga tacgctttcg
    39841 taaagcaata gtaagctgat attctaccac actatgagca aaacacattt aacagaacag
    39901 aagttttccg acttcgccct gcatccgaag gttgtagaag cccttgaaaa aaaagggttt
    39961 cataactgta cgcccattca ggcactggcc cttccgctga cgctggcggg tcgtgacgta
    40021 gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt ttcttacgtc aacgtttcat
    40081 tatcttctct ctcatcctgc gattgccgat cgcaaggtga atcagccgcg tgccttaatt
    40141 atggcaccga cgcgtgaact tgccgtgcag atccatgccg atgcggaacc gctggcggaa
    40201 gctaccggcc tgaagctggg tctggcttac ggtggtgatg gttacgacaa acagctgaaa
    40261 gtgctggaaa gcggcgttga cattctgatt ggcaccacgg gtcgtttaat tgactacgcc
    40321 aagcagaacc acattaacct cggtgccatt caggtagtgg tactggacga agccgatcgc
    40381 atgtacgatc tgggctttat taaagatatc cgctggctgt tccgccgtat gccgcctgca
    40441 aaccagcgcc tcaacatgct gttctccgcc acgctttcgt accgggtacg tgaactggcg
    40501 ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac cggaacagaa aacgggccac
    40561 cgtattaaag aagagctttt ctacccttct aacgaagaaa aaatgcgttt gctgcaaacg
    40621 ctgatcgaag aagagtggcc agacagagcg attattttcg ccaataccaa acaccgttgt
    40681 gaagagatct ggggccacct ggcggcagat ggtcatcgtg tcggtttgtt gacaggcgat
    40741 gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta cccgtggcga tctggatatt
    40801 ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc cggcagtgac acacgtcttt
    40861 aactacgatt tacccgatga ctgtgaagat tacgttcacc gtattggtcg tacaggtcgc
    40921 gcaggcgcaa gcggtcactc tattagcctg gcgtgtgaag agtatgcatt gaatttgcct
    40981 gctattgaga cctatattgg tcactcaatt ccggtaagca aatacaatcc ggacgcattg
    41041 atgaccgatc tgccaaaacc gctgcgcctc acgcgcccgc gcacaggcaa tggtccgcgt
    41101 cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa taaaaaatgt catactcaaa
    41161 atcattcaaa ctgtatcaag atggcaagag aatgaatccc gatgagctta ctgtagtaag
    41221 tgattcaggt gaaagaatgc agccaacaca gagacagatt gaaggatgaa gagtatgggt
    41281 tccacctcgt cgctgtatgc agccattgat ctcggttcga atagttttca tatgctggtt
    41341 gtgcgcgagg tggctggaag catccagacg ctgacgcgaa ttaaacgcaa agtgcgtctg
    41401 gctgctggcc tgaatagcga aaatgccctc tctaatgaag caatggagcg cggttggcaa
    41461 tgtctgcgcc tgtttgctga acgtctgcaa gatatccctc cctcgcaaat tcgtgttgtc
    41521 gccacggcga cgttacgcct tgccgtcaat gcgggtgatt ttattgccaa agcgcaggaa
    41581 attctcggtt gtccggtaca ggtgatcagc ggtgaagagg aagcacgtct gatttatcag
    41641 ggcgttgctc acaccactgg tggtgccgat cagcgcctgg tggtggatat aggcggagcc
    41701 agtactgaac tggtaaccgg cacgggtgca caaaccacct cgttgttcag cctgtcgatg
    41761 ggctgcgtca cctggctgga acgctatttt gccgatcgta atctggggca ggaaaatttt
    41821 gatgctgcag aaaaagcggc acgcgaagtg ttacgtccgg ttgccgatga attacggtat
    41881 cacggctgga aagtgtgcgt tggtgcttcc ggcaccgtgc aggcgttaca ggaaatcatg
    41941 atggcacagg ggatggatga acgcattacc ctggaaaagt tgcagcaatt gaaacagcga
    42001 gccattcatt gcggtcggct ggaggaactg gagattgacg ggctgacgct ggaacgtgcg
    42061 ttagtgttcc cgagtggtct ggcgatcctg atcgccattt ttaccgaact gaatattcag
    42121 tgtatgaccc tggcgggcgg tgcgctgcgt gaaggcctgg tctacggcat gttgcatctg
    42181 gccgtcgagc aggatattcg cagccgtacg ctgcgtaata ttcaacgccg ctttatgatc
    42241 gatattgatc aggcacagcg cgtagccaaa gttgcggcta acttcttcga tcaggtggaa
    42301 aacgaatggc atcttgaagc aataagccgc gatttgctca tcagcgcctg tcagcttcat
    42361 gaaatcggcc tgagcgttga cttcaaacaa gcgccgcaac acgctgctta tctggtgcgt
    42421 aatctggatc ttcccggttt tacccccgca cagaaaaaat tgctggcgac gctactgctc
    42481 aaccagacta atccggtcga tctctcatcg ctgcatcagc aaaatgccgt accgccgcgc
    42541 gtcgcagaac aactctgccg tttactacgc ctggccatca tttttgccag ccgtcgccgt
    42601 gacgatctcg tgccagagat gacattacag gctaaccatg aactgttgac cttgacgctt
    42661 ccgcaagatt ggctaaccca acatccgctg ggtaaagaga ttattgctca ggaaagccag
    42721 tggcagagct atgtccactg gccgctggaa gtgcattaat gagtaagtgc cggatgcgat
    42781 gctgacgcat cttttccggc cttgattatt tccctcgttt tgccgccatc atcgctttca
    42841 gattcgccag atggctttgc cctttctgca tccgttcttc ggcgctgacc actttgcgct
    42901 cctgttccca aatcagatca tcctgcggca gctccagcaa aaagcggctc ggctccgggc
    42961 gcaccagttc gccgtactga cggcgttctt tacacagcgt aaaggtcaat tccttctggg
    43021 cgcgggtaat gccgacatag gccagccgcc gctcctcatc gatattatct tcatcgatgc
    43081 tgctctggtg cggcaaaaac ccttcttcca taccgaccat gtagacataa ggaaactcca
    43141 gccctttcga cgcgtggaga gtcatcagtt gcacctgatc cagctcttct tcactctcac
    43201 cacgctccat catgtcgcgc aaagtaaagc gcgtcaccac ctgggttaac gtcatcggct
    43261 catccagttc actgccttcg agcatctccg tcatccagct aaacagttgg ttgacgttct
    43321 tcatgcgcat ttcggcggct ttcgggctgg gcgatgtttc gtacagccag gattcataat
    43381 ccatgccgtg gatcagatcg cgcaccgcgg caatcggctc ccgctccgcc aggcgctgga
    43441 tttctgccaa ccagtgagtg aagcgggtca atgcttcata accacgtccg ctaagcgtct
    43501 gactcaggcc catatcaaag ctggcggtaa acatgctttt attgcgcgtc atcgcccatt
    43561 cacccaactt ttttagcgtc gccgggccaa tctctcgctt cggcgtgtta acgatacgca
    43621 gaaatgcgct gtcatcgtcc gggttagtca gcacgcgcag ataagccagc aagtccttga
    43681 tttcaggacg agagaaaaac gacgtaccac cagatatttt gtacgggatg cggttttgca
    43741 tcaggaattt ttcaaacacc cgcgactgat ggttgccgcg ataaagaatg gcgtaatctt
    43801 tgtactgcgt tttattgacg aagtgatggg cgatcagctc gccagtaacg cgctcggctt
    43861 catgttcctc gttattcgcg cttaaaactt ttagctctgt gccgtaaccc agttcggaga
    43921 acagacgctt ttcaaagacg tgcgggttat tggcgatcag gatgttcgcc gctttcaaaa
    43981 tacgtccgga agagcggtag ttctgctcaa gcttaatcac cttcagcgcc ggaaaatcct
    44041 gactcagcag caccaggttt tgcggacgtg caccgcgcca ggagtagatc gactggtcgt
    44101 catcgcccac cacggtaaag cgcgcgcggc tgcccaccag cagtttcacc agctcatact
    44161 ggctggtgtt ggtgtcctga tactcatcca ccagcagata gcgaattttg ttctgccagc
    44221 gctcgcggac ttcttcattg cgttgcagca gcaacgtcgg caataaaatc agatcatcga
    44281 agtcgagaac gttacaggct ttcaggtgtg catcatacag cccataacaa tgggcaaaaa
    44341 tacgatcccg ctcgcccttt gcctctgccg ccgcctgcgc cggagttttg agatcgttct
    44401 tccagttaga gatggtcgaa atcagttgtt gcaggagaac tttgtcatct tcaatcagcc
    44461 cctcggtcaa ctctttaagc aaagcaagct gatcggtatc gtcaaacaac gagaagttcg
    44521 ctttcatccc aagcgccgca tactcgcgtt tgatgatatc cagccccaac gtatggaaag
    44581 tggagatcat cagcccacgc gcctctttgc gccccagcgt ctgccctaca cgctctttca
    44641 tctcgcgcgc cgccttattg gtaaaggtta ccgccgcgat atgtcgcgcc tgataaccgc
    44701 aaccgcggat cagatgggcg attttattgg tgataacacg agttttaccg gaacccgcgc
    44761 ccgccagcac caggcagggg ccggtaacga attcgacagc ttgttgttgg ccggggttta
    44821 gacgcatagg tgtattgctc aatcttcgaa cggggggagg attgtagcag aatcgcattt
    44881 gaatgtgacc gcgtgtaagc cgctaaccgg ttggctgcgg atgtttaaat cagagagaga
    44941 agaccgaaat cggagttcat cgtgaatcca ttagacttag aaaatatcgg gtcgtaacgt
    45001 tgtgttatta ctttttcaga gtttactaag gagaggttta ctcgtggcga ttaatttcag
    45061 ccctaaggtt ggtgaaatac tggaatgcaa ctttggtaac tatcctgtta gccaaaatgg
    45121 accgttcagc acgacgtatt acgatggccg cattcctcct gaaatgataa aaaacaggtt
    45181 agttgtcgtg ctcaacggaa aaattaatgg caacgcatgt atcgtcgttc cattatcaac
    45241 cactcgcgac catgacaagt taaaacgtgg aatgcatgtt gaaatagcca gtaatgttat
    45301 caatgacctg caattctttg atcagcagat tcgttgggcc aaaaccgacc tggtacaaca
    45361 agtcagtcga aacagattaa acagagcaag aacttacaga gggtatctta atcaatgttt
    45421 gccacatgag ttggttgcag acatacagag ggcggtcatc aagtcaatca atgccatatc
    45481 gctcataaat tgacaataaa atcaccatcc ccgataatga ctttgtagcc catagaggcc
    45541 ctaagttttc tccccttgag ggacctgcga agcccggatt ttccgggctt ttgccatata
    45601 tgtaccagcc cctccctgac tgacaattac gttagcatct gcggatcatg ctaaaatcgc
    45661 cgcctgacaa ttattcactc aaggcacgat catggcaaaa acagcagcag cactgcatat
    45721 ccttgtaaaa gaagagaaac tggctctgga tcttctcgag cagattaaga acggggccga
    45781 ttttggcaag ctggcgaaga aacactccat ttgcccatca ggcaaacgcg gcggcgattt
    45841 aggtgaattc cgccagggtc agatggttcc ggcgttcgat aaagttgtgt tctcttgtcc
    45901 ggtactggag ccgactggcc cgctgcatac ccagttcggt taccacatca ttaaggtgct
    45961 ttaccgtaac taacagtaag gccttctcca ggagaaggcc ttgaactctg aatcgctatt
    46021 aacccgcaac agcaatgcgt ttcatatctg tcatatagcc gcgcagtttc ttacctacct
    46081 gctcaatcgc atggctgcga atcgcttcgt tcacatcacg cagttgcgcg ttatctaccg
    46141 cgccttccgg aatagcttta cccaggtcgc ccggttgcag ctctgccata aacggtttca
    46201 gcaacggcac acaagcgtaa gagaacagat agttaccgta ctcagcggta tcagagataa
    46261 ccacgttcat ttcgtacaga cgcttacggg cgatggtgtt ggcaatcagc ggcagttcgt
    46321 gcagtgattc atagtaagca gattcttcga tgatgccgga atcaaccatg gtttcaaacg
    46381 ccagctcaac gcccgctttc accatcgcga tcatcagtac gcctttatcg aagtactcct
    46441 gctcgccgat tttgccttca tactgcggcg cggtttcaaa cgcggttttg ccggtttctt
    46501 cacgccaggt cagcagtttc ttatcatcgt tggcccagtc cgccatcatg ccggaggaga
    46561 attcgccgga gatgatatcg tccatatgtt tctggaacag cggtgccatg atctctttca
    46621 gctgttcaga cagcgcgtaa gcacgcagtt tcgccgggtt agagagacgg tccatcatca
    46681 gggtgatgcc gccctgtttc agcgcttcgg tgatggtttc ccagccgaac tgaatcagtt
    46741 tttctgcgta tgccggatcg gtgccttctt ccaccagctt gtcgaagcac agcagagagc
    46801 cggcctgcaa cataccgcac agaatggttt gctcgcccat caggtcagat ttcacttccg
    46861 caacgaagga cgattccagc acgcccgcac ggtgaccacc ggttgctgcc gcccatgctt
    46921 tagcgatcgc cataccttcg cctttcggat cgttttccgg gtgaacggca atcagcgtcg
    46981 gtacgccgaa tccacgtttg tactcttcac gcacttcggt gccagggcat ttcggcgcaa
    47041 ccatgactac ggtgatatct ttacggatct gctcgcccac ttcgacgatg ttgaaaccat
    47101 gcgagtaacc cagcgccgcg ccgtctttca tcagtggttg tacggtgcgc actacatcag
    47161 aatgctgctt gtccggcgtc aggttaacca ccaaatccgc ctgcgggatc agttcttcgt
    47221 aagtaccaac tttaaaaccg ttttcggtcg ctttacgcca ggatgcgcgc ttttcggcaa
    47281 tcgcttcctt acgcagcgcg taggagatat ccagaccaga atcacgcatg ttcaggccct
    47341 ggttcagacc ctgtgcgcca cagccgacga tgaccacttt tttactctga aggtagctcg
    47401 cgccatcggc gaattcgtcg cggcccataa agcgacattt gcccaactgt gccagttgct
    47461 ggcgcaggtt cagtgtattg aagtagttag ccatggtgat tcctcgtgat gttgtgcttc
    47521 ttattgttcg gttcgcgttt gcgagattga attcactata tgacaggaaa tttattgcgg
    47581 aaattgatat attcacaacg tcatattgca atttttgcaa cgtcaacatc gagggctgtc
    47641 cctgtggatt tacgcgatct gaaaaccttc ctgcatctgg cggaaagccg ccattttggc
    47701 cgcagcgcgc gggcgatgca cgttagccca tccacgctct cacggcagat tcagcgcctg
    47761 gaagaagatc tcggtcagcc gctgtttgtg cgcgataacc gcacggtgac gctgactgaa
    47821 gcgggcgaag aactgcgcgt tttcgcccag caaacgctgt tgcaatatca gcaattgcgc
    47881 cacaccatcg accagcaagg accgtcgctc tctggcgaat tacatatctt ctgctcggtg
    47941 accgctgcct acagccatct gccgccgatt ctggatcgct tccgcgcgga acatccgtcg
    48001 gtggagatca aactcaccac tggtgatgcg gcagatgcaa tggaaaaagt ggtcacggga
    48061 gaagcggatc tggcgattgc cgggaaaccg gagacgctac ccggcgctgt ggcgttttcg
    48121 atgctggaga atctggcggt agtgctgatt gcgcccgcgc tgccctgccc ggtgcgtaat
    48181 caggtctcgg tagagaagcc ggactggtca acggtaccgt ttatcatggc cgatcagggg
    48241 ccagtacgcc gccgcattga actgtggttt cgacgcaata aaatcagcaa cccaatgatt
    48301 tacgccacgg ttggcgggca tgaagcgatg gtatcgatgg tggcgctcgg ctgtggtgtg
    48361 gcgctgttgc cggaagtggt gctggaaaac agcccggagc ctgtacgcaa ccgggtaatg
    48421 attttagatc gcagcgatga gaaaacgccg tttgaactgg gcgtgtgtgc acaaaaaaag
    48481 cggctgcatg agccgctgat tgaggcgttc tggaggattt tgccgaacca caaatgacgt
    48541 tgtcgcgcga gtaggcctga taagcgaagc gctatcaggc atttttccct aacccgccaa
    48601 aaagaacctg aacgccgggt tattggtttc gtcgtggcaa tcgtagccca gctcattcag
    48661 ccgggtttcg aaatccggtt catggtcgcc aagttcgaac gccgccagta cacgcccgta
    48721 gtcggtaccg tgactgcgat aatggaacag agaaatattc cagtgcgtac ccagcgtgtt
    48781 gaggaagcgc agcagcgcac ccggcgattc cggaaattcg aagctgtaga gacgttcctg
    48841 caacggatgc gatggacgcc ccccaaccat atagcgcacg tgtaacttcg ccatttcgtc
    48901 gtcggagaga tcaaccacgc tgtagccgcc gtcgttgagc atctgcaaaa tttctttgcg
    48961 ctcttcgagg ccacggctta agcgcacacc gacaaagatg caggcgtttt tggcatcggc
    49021 aaagcggtag ttgaattcgg tgaccgaacg tccgccaagc agttggcaga atttgaggaa
    49081 gctacctttc tcttccggaa tggttactgc cagcaacgct tcacgctgtt cacccagttc
    49141 acaccgttcc gagacgtagc gcaggccgtg gaagttcacg ttggcaccgg aaagaatatg
    49201 cgccagccgt tcgccgcgaa tgttgtgctg ggcgatatat tttttcattc ccgccagcgc
    49261 cagcgcgcca gagggttccg ccaccgcgcg cacatcttcg aataaatcct tcatcgccgc
    49321 acagatcgca tcactatcga cggtgatgat atcgtcgaga tactcctggc acaaacggaa
    49381 ggtttcgtcg ccgatgcgtt ttaccgcaac gccttcagca aacagcccca cgcgcggcag
    49441 atcaaccgga tgacccgcat ccagcgctgc tttcaggcag gcggagtctt ccgcttctac
    49501 ggcgattact ttgatttgcg gcatcagttg tttgatcagc accgccacgc cagcagccag
    49561 accgccgccg ccgactggca caaatacgcg gtcgagatgg gcgtcctgct ggagcagttc
    49621 cagcgccagc gtgccttgcc cggcaatcac catcggatgg tcgaacggcg gcacccaggt
    49681 gaacccctgc tgctgtgaca gttcgatcgc tttggctttc gcttcatcaa agttcgcgcc
    49741 gtggagcagc acttcgccgc cgaagccgcg caccgcgtcg actttgatgt cggcggtggc
    49801 ggttggcata acgatcaggg ccttcacgcc taaccgcgca gaagaaaacg cgacgccctg
    49861 cgcgtggtta cccgcagaag cagtgatcac gccgtgcgct ttttgttctt ccgtcaggcc
    49921 cgccatcatg gcgtatgcgc cgcgcagctt aaagctgtgc actggctggc gatcttcgcg
    49981 tttcaccaga atcacgttat caagacgaga cgagagcttt tccatttttt gtagcggcgt
    50041 gacctgcgcc gcttcgtaga ccggcgcgcg cagcaccgct cttaaatatt cagcaccttc
    50101 cggggcaccg gacaggggtt gcgagtcagc cattattacc cccccagttt cgatttatcg
    50161 cgcaccgcgc ctttgtcggc gctggttgcc aggctggcat aagcacgcag ggcaaaggag
    50221 acctgacgtt cacgattttt cggcgtccag gctttgtcac cccgagcttc ctgcgcttca
    50281 cgacgcgccg ccagttcggc atcgcttacc tgtaactgaa tgccacggtt cggaatgtcg
    50341 atggcgatca ggtcgccgtc ttcaatcagg ccaatgctgc cgccgcttgc cgcttccggt
    50401 gagacgtggc cgatagaaag gccagaggtg ccgccagaga aacgaccgtc ggtgatcagc
    50461 gcacaggctt taccgagccc cattgatttc aggaagctgg ttgggtagag catttcctgc
    50521 atccccggac caccttttgg gccttcatag cgaattacta ccacatctcc ggcgacaact
    50581 ttaccgccga gaatcgcttc taccgcgtcg tcctggcttt cgtatacttt cgccgggccg
    50641 gtgaatttga ggatgctgtc atcgacgccc gcggttttaa cgatgcagcc gttttctgcg
    50701 aaattaccgt agagcactgc caggccgccg tctttgctat aggcgtgttc cagcgagcgg
    50761 atacagccat tggcgcgatc gtcgtccagc gtatcccaac ggcaatcttg cgagaatgcc
    50821 tgggtggtac gaatgcccgc cgggccggcg cggaacatat tttttaccgc gtcatcctgg
    50881 gtcagcataa cgtcgtattg ttccagcgtt tgcggcaacg tcaggccaag tacgtttttc
    50941 acatcacggt tcagtaaccc cgcacgatcc agctcgccga gaataccgat aacaccacca
    51001 gcacggtgaa catcttccat atggtatttt tgggtgctcg gcgcgacttt gcataactgc
    51061 ggaaccttgc gggaaagctt atcgatatca ctcatggtga agtcgatttc cgcttcctgt
    51121 gccgccgcca gcaggtgaag tacggtgttg gtagagccgc ccatcgcgat atcgagcgtc
    51181 atagcgtttt caaacgccgc cttactggcg atattacgcg gcagtgcact atcgtcgttt
    51241 tgctcgtagt aacgtttggt caattcaaca atgcgtttac cagcattgag gaacagctgc
    51301 ttacggtcag agtgggttgc cagcagcgag ccgttgcccg gctgcgacag acccagcgct
    51361 tcggtcaggc agttcattga gttagcggta aacatcccgg agcaggaacc gcaggttggg
    51421 catgcggaac gttcaacctg atcgctctgg gagtcagata ctttcgggtc tgcgccttgg
    51481 atcatcgcat caaccagatc gagcttgatg atctgatcgg acagtttggt tttcccggcc
    51541 tccatcgggc cgccggaaac aaagatcacc ggaatattca ggcgcagaga ggccatcagc
    51601 atccccgggg tgattttgtc acagttagag atgcagacca tggcgtcggc gcagtgggcg
    51661 ttgaccatat actcaacgga atcagcgatc agttcgcgag atggcagtga ataaagcatc
    51721 cccccgtggc ccatggcaat cccatcatcc accgcaatgg tgttgaactc tttggcaacg
    51781 ccgccagccg cttcaatttg ttcggcgacc agtttaccga gatcgcgcag atggacgtga
    51841 cccggtacaa attgggtgaa cgagttcaca accgcgataa tcggcttacc gaaatcggcg
    51901 tcggtcattc cggtggcgcg ccacagcgca cgagcacccg ccatattacg accatgagtg
    51961 gtggtggcgg aacggtactt aggcatactt tatttactcc cagtgtctgt ctcgtaaatg
    52021 ggacggtgcg tgccgtccca ttttttgtat ttattgatta acttgatcta accagcccca
    52081 tttatcttcg gtttcgccgg taaagaggcc gaagaaggct tgctgaatgc gtttggtgac
    52141 cgggccacaa cggccttcgc caacctgaat accgtctaca ctgcgcactg gcgtgatttc
    52201 tgccgccgta ccggacataa agacttcatc cgccaggtac agggattcgc gcgacagcac
    52261 ctgctcacgc acttcaattc ccagctcttt cgccagtttg atgatggcat cacgggtaat
    52321 acccggcagc gcggaggagg tgaacggtgg ggtgaacagc acaccatctt tcacttcaaa
    52381 caggttttcg cctgcgcctt cagagatata accgttcaca tccagcgcga taccttcctg
    52441 ataaccgtgg cggcgcgctt cgctacccac cagcagggaa gagagatagt taccaccggc
    52501 ttttgccgca gttgggatgg tgtttggtgc tgcgcggttc caggaggaaa ccatcgcatc
    52561 gatcccctgc tccagcgctt ctgcgcccag atacgctccc cacgggaaag cggcgataat
    52621 cacatcggtt gagtatcccg ctggcgggtt tacgcccatg ccaacatcac caacgaagat
    52681 cagcggacgg atataggcgc tggtgagatt gtttttgcgg atcacgtcac gacaagcttc
    52741 catcagctca tcaatgctct gcgaaaccgg gaagcgatag attttggcgg agtcatgcag
    52801 acgctgcata tgctcacgat ggcggaatac aaccggtcct ttgtgcgagt cgtagcaacg
    52861 gatgccttca aaaaccgagg tgccatagtg cagcgcgtgc gacatcacat gcaccttcgc
    52921 gtcttcccag cgaaccatct ccccattgaa ccaaatgtaa tcagctttct tcgtggtcat
    52981 ttttatattc cttttgcgct caggcgcgga tttgttgtga tgtggttgtg ctctggcaga
    53041 tggcaacgtg tgcgacgtcc accagtttat ttaactgact aaacagtaag tcgaccgacc
    53101 gtgggctggc aacggtcaat tcgatattta tattttgtgc atcgctggcg gcggccatat
    53161 tcattgagca gacgtggaaa ccacgatgac gcaccacgcg taaaacacgt tctaaggttt
    53221 ctggattgaa gcgagccgat acattgacct gatgttgcat catgataatt tctccaacat
    53281 ttctgaatta ctggcgccag gcggcaccag cggccagacg ttctcaagtt cgtcgattga
    53341 gacatgaagc aggtatggcc catcactgtt cagcatggtg tcgagtgccg cttcaacctg
    53401 gtctttacgg gtgatgtgtt ggccagggat gccgaaggcg ctggctaaca tgaggaaatc
    53461 ggggttatca gtaagggtgg tttcgctgta tcgttcctga aaaaacagtt gctgccattg
    53521 tcgaaccatc cctaaccgtt ggttatcgag taagacgatt ttcaacggta actgcttgcg
    53581 ttttacggtg cccagctctt gcacattcat catgaaagag ccgtcaccgg agatacagac
    53641 aacggtatcg ttcggtcgcg cgacttgtgc gccaaccgcc gccggtaaac caaaacccat
    53701 ggtacctaag ccgctggagg tgatgaaatt ttccgggcga gtgtgggcga tgtgctgcgc
    53761 agcccacatc tggtgctgcc ccacatctgt ggtcacgacg caatccgcag gtttacgatc
    53821 cgacagttgt tttaacaaca acggcgcgta gatagcgtca ccgggatggt cgtaacgcca
    53881 ggcatgttca tcacgcagct gcgcgcagtg tagctgccag tcattgatat ttaacggctg
    53941 ctgtaatgct ggtaacagag catttaaatc accttgtaat gccacatgtg cctgacgcag
    54001 cttgttcatt tctgccgggt cgatatccat atggataaca ctggcgtgtg gtgcgaaggt
    54061 gttcagtttg ccggtcaccc ggtcatcaaa acgtgcaccc acggcgatca gcaagtcgca
    54121 ctcctgcacc gcgaagttcg ccgctttggt gccatgcatt cccagcatgc ccagatagta
    54181 cggataatct gcttcaactg cgcccagccc tttcagcgtg caggtggcag gcatttttgt
    54241 ggtagcgaga aattctcgta aagcaggaac tgcctgcgcc atacccacgc caccaccaac
    54301 gtacagcatc ggtttttgcg cttttgccag catctggcgc gcttgctcaa cttcggcatg
    54361 tgggaaagtc acttcgtttt caacggtggt gaaccacggt tccaggtcgc cgctggctaa
    54421 ctggatatct tttgggatat cgaccagaac cggaccagga cgacctgagc tggcaacgtc
    54481 gaatgcttca gccataatgc gcggcaactc ttccagcgac tgcaccagaa agctgtgctt
    54541 ggtacaggct aacgacaatc ccaggacatc cacttcctga aatgcgtcag tgccgataaa
    54601 cggtgcggac acttgaccgg tgatggcaac aacagggata gaatctaaca gtgcgtccgc
    54661 aagcccggtt atcaggttgg ttgcgcccgg accagacgtg gcgatacata cgccagtttt
    54721 gccggtagca cgggcataac cgatagccgc cattgccgca ccctgctcat gtcggcacag
    54781 caagtgctcc acgccgccgt catacaatgc atcgtaaacc ggcataattg cgccacccgg
    54841 ataaccgaaa acggtgttca caccctgtgc ccgcaacgca tgtaccaccc actgtgcgcc
    54901 attcatagtt agttccccgt cctgaatctt gagaaacaga attttgtgct gttattcatt
    54961 gtctgctcct cggttatgtt tttaaggtca aaaaaaaccc ccggaccttt cggtgcgggg
    55021 gtcttagttc gttaaggctt gatctctaag cctttcctcg tccaagtgca gccccgcacg
    55081 gtgggataat aatcaccacc acgctaatca cgaccaggct aatcactcgt agaagggctg
    55141 tcattttgtc ttttcttgca tcttgttcga aggaatgcct aaagagttac catagatttt
    55201 gcgaatagca caagatattt tttggccgtt ttttatgatg gctttaataa tttcaataga
    55261 aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaaa ttcaatttca gtacgaatgt
    55321 tgttccctcc ctcgtagttc atcctttgtc cgctgcattt ttgcgagcat cattccaccg
    55381 ccagaacaac tcaacttcac ttgcacaaat aaacaggaat cagccactaa aattcacgaa
    55441 ttagcgggct acagcagcga tatcttatgc acataatggt gactcaagga gggcttatgt
    55501 cactgtcaat tgttcatacc cgcgcagccc tgggagtaaa tgcgccccca atcactgttg
    55561 aggtacatat cagtaaaggt ctacccggtt taacgatggt gggcttacca gaaacaacgg
    55621 taaaagaagc ccgcgatcgc gtgcgcagcg ccattatcaa tagcggatat gaatatccgg
    55681 ccaaaaaaat taccatcaac ctggctccag ccgatctacc aaaagaaggg ggacgatatg
    55741 atttacctat cgccattgcg ttgctggcgg cctcagaaca gcttacagcc aataagttag
    55801 atgaatatga attagtcgga gaactggcgc ttacaggcgc tctgcgtggc gttcccggtg
    55861 caatctccag tgcaactgaa gctattaagt cgggcagaaa aattatcgtc gcgaaagata
    55921 acgaagatga agtggggcta attaacggtg aaggatgcct gatagccgat catctgcagg
    55981 ctgtctgtgc gtttctggaa ggtaagcaca ctctcgaacg cccgaaacca actgatgcag
    56041 tatcccgggc gctacaacat gatctcagtg atgttgtcgg tcaggagcaa ggaaagcgag
    56101 gactggaaat taccgccgct ggtgggcaca accttttact gattggaccg ccgggaacag
    56161 gtaaaacaat gctcgccagc cgtattaatg gcctgttgcc agatttaagc aatgaagagg
    56221 cactggagag cgctgcgata ttaagtctgg taaatgctga atcagtacaa aaacaatggc
    56281 ggcagcgccc gttccgctca cctcatcaca gtgcgtcgtt aactgcgatg gtgggcggtg
    56341 gtgcaattcc agggccaggt gaaatttcgc tggcgcataa cggcgtgctt tttcttgatg
    56401 agttacctga atttgaacgg cgtacactgg atgctttgcg agagccgatt gaatccgggc
    56461 agatccatct ttcacgcaca cgagcaaaaa taacctatcc agcccgtttc cagcttgttg
    56521 cggcgatgaa tcccagccct accggacatt atcagggaaa ccataaccgc tgcacgccag
    56581 aacagacatt acgttatctc aaccggctct cggggccctt tctcgaccgc ttcgatctct
    56641 cactggagat cccattacca ccccccggca ttttgagtaa aacggtagtg ccgggagaaa
    56701 gcagcaccac cgttaaacaa cgtgtaatgg ctgccagaga gcgccaattt aagcggcaga
    56761 ataagttgaa cgcctggctg gatagtccag aaatacgcaa attctgcaag cttgagagcg
    56821 aagatgcgca gtggctggaa gaaacgctga tccatctggg gttatcgatt cgtgcctggc
    56881 agcggttatt gaaagttgca cgaaccattg ctgatattga tcagtctgac attatcacac
    56941 gtcagcattt gcaggaggca gttagctatc gtgcgattga ccgtttgctc atccatctgc
    57001 aaaaactact gacataaaaa aagggcattt cgcccttttt attaatcgtc agaatcggtg
    57061 tagtcttcag caccttcaac ctgcggttta ccgccggaaa gggtgtgaaa acgttttgga
    57121 cgcttgatac gcgtcatata cttggaccac acgcgttctg cttctgtcac tggctcacgt
    57181 tcgccacggc atactgctac gaagagtttc tcttcctcgg taaccggctc gcgtttgcca
    57241 agatccaact cattgaaggc ataaccatga cgctcaagca gttgtgcctc tttgatggtg
    57301 aaatcaccat ggcgagagaa tccacgtgga taatgtttat tgtcgaaata tcgattagtc
    57361 gtcgtaaagc tttccgccat cctgcacgct cctaattctt tgaccgagct agttatggcg
    57421 cggagtatta gttacgcttg acagagtgta aaacaaaaca tttaaatcat aacgacaaat
    57481 aattttgcgg agagcactgt ggatacggaa ttgttaaaaa ctttcctgga agttagccga
    57541 acgcgtcact ttggtcgagc ggctgaatcg ctctatctga cccagtcagc agtgagcttt
    57601 cgaatcagac aactggaaaa tcaactgggt gtgaaccttt tcacccgcca cagaaacaat
    57661 atccgtttaa ccgctgccgg tgaaaaacta ctgccttatg cagaaacgct catgagcacc
    57721 tggcaggccg cccgtaagga ggtggcgcat acctcacgac ataacgagtt ttctatcggt
    57781 gccagcgcct cattgtggga atgtatgctt aatcagtggc tgggacgttt gtatcaaaat
    57841 caggatgccc atacaggctt acagttcgaa gcgcgaattg cccaacggca gtctctggta
    57901 aaacagctgc atgaacgcca gcttgatctt cttattacca ctgaagcgcc caaaatggac
    57961 gaatttagta gtcagttgct ggggtatttc actttagcgc tttataccag tgccccttct
    58021 aaactaaagg gagatcttaa ctatctgcga ctggagtggg ggccagattt tcaacagcat
    58081 gaggcaggtt tgatcggtgc tgacgaagtg cccattctga caaccagttc tgctgaactg
    58141 gcacagcaac agattgcgat gcttaatggt tgcacctggc tacccgtcag ctgggcgcgt
    58201 aaaaaaggcg gcctgcatac cgttgtcgat agcacaacac tttcacggcc gctttatgcc
    58261 atatggctgc aaaatagcga taaaaatacg ttgattcgcg atctattgaa aattaacgtg
    58321 ctggatgaag tgtattaatc tgaatggctg gcaaggatgc cggtagaagg atttacttca
    58381 gagagggtta tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggcagg
    58441 ggcggagaga ctcgaactcc caacacccgg ttttggagac cggtgctcta ccaattgaac
    58501 tacgccccta attagggtgg cggaacggac gggactcgaa cccgcgaccc cctgcgtgac
    58561 aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca accaccggtt
    58621 ttatgaccgg cttactgctt
//
LOCUS       DAZUCE010000006        53292 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZUCE010000006 DAZUCE010000000
VERSION     DAZUCE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.006"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            313..1629
                     /gene="fucP"
                     /locus_tag="ACHY03_000339"
     CDS             313..1629
                     /gene="fucP"
                     /locus_tag="ACHY03_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFS3903620.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1662..3437
                     /gene="fucI"
                     /locus_tag="ACHY03_000340"
     CDS             1662..3437
                     /gene="fucI"
                     /locus_tag="ACHY03_000340"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFS3903621.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3546..4964
                     /gene="fucK"
                     /locus_tag="ACHY03_000341"
     CDS             3546..4964
                     /gene="fucK"
                     /locus_tag="ACHY03_000341"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFS3903622.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4966..5388
                     /gene="fucU"
                     /locus_tag="ACHY03_000342"
     CDS             4966..5388
                     /gene="fucU"
                     /locus_tag="ACHY03_000342"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFS3903623.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5446..6177
                     /gene="fucR"
                     /locus_tag="ACHY03_000343"
     CDS             5446..6177
                     /gene="fucR"
                     /locus_tag="ACHY03_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFS3903624.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATYLVTA"
     gene            complement(6221..7321)
                     /gene="rlmM"
                     /locus_tag="ACHY03_000344"
     CDS             complement(6221..7321)
                     /gene="rlmM"
                     /locus_tag="ACHY03_000344"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFS3903625.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7314..7709)
                     /locus_tag="ACHY03_000345"
     CDS             complement(7314..7709)
                     /locus_tag="ACHY03_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFS3903626.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7728..8645)
                     /gene="gcvA"
                     /locus_tag="ACHY03_000346"
     CDS             complement(7728..8645)
                     /gene="gcvA"
                     /locus_tag="ACHY03_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFS3903627.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(8996..9223)
                     /locus_tag="ACHY03_000347"
     CDS             complement(8996..9223)
                     /locus_tag="ACHY03_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFS3903628.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9415..10620
                     /gene="csdA"
                     /locus_tag="ACHY03_000348"
     CDS             9415..10620
                     /gene="csdA"
                     /locus_tag="ACHY03_000348"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFS3903629.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10620..11063
                     /gene="csdE"
                     /locus_tag="ACHY03_000349"
     CDS             10620..11063
                     /gene="csdE"
                     /locus_tag="ACHY03_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFS3903630.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(11114..11920)
                     /gene="tcdA"
                     /locus_tag="ACHY03_000350"
     CDS             complement(11114..11920)
                     /gene="tcdA"
                     /locus_tag="ACHY03_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFS3903631.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12159..13256)
                     /gene="mltA"
                     /locus_tag="ACHY03_000351"
     CDS             complement(12159..13256)
                     /gene="mltA"
                     /locus_tag="ACHY03_000351"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFS3903632.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13465..13541
                     /locus_tag="ACHY03_000352"
     tRNA            13465..13541
                     /locus_tag="ACHY03_000352"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13499..13501,aa:Met,seq:cat)
     gene            complement(13615..14868)
                     /gene="amiC"
                     /locus_tag="ACHY03_000353"
     CDS             complement(13615..14868)
                     /gene="amiC"
                     /locus_tag="ACHY03_000353"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFS3903633.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15100..16431
                     /gene="argA"
                     /locus_tag="ACHY03_000354"
     CDS             15100..16431
                     /gene="argA"
                     /locus_tag="ACHY03_000354"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFS3903634.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16493..18319)
                     /gene="recD"
                     /locus_tag="ACHY03_000355"
     CDS             complement(16493..18319)
                     /gene="recD"
                     /locus_tag="ACHY03_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFS3903635.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTIAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDANTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18319..21861)
                     /gene="recB"
                     /locus_tag="ACHY03_000356"
     CDS             complement(18319..21861)
                     /gene="recB"
                     /locus_tag="ACHY03_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFS3903636.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDEARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIVENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDVLTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VREKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSARNKQVEMEFYLPISEP
                     LIASQLDTLIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQVHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(21854..24742)
                     /gene="ptrA"
                     /locus_tag="ACHY03_000357"
     CDS             complement(21854..24742)
                     /gene="ptrA"
                     /locus_tag="ACHY03_000357"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFS3903637.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKSANIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24918..28286)
                     /gene="recC"
                     /locus_tag="ACHY03_000358"
     CDS             complement(24918..28286)
                     /gene="recC"
                     /locus_tag="ACHY03_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFS3903638.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMTQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLANRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(28299..28622)
                     /locus_tag="ACHY03_000359"
     CDS             complement(28299..28622)
                     /locus_tag="ACHY03_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFS3903639.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVVIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(28607..29014)
                     /locus_tag="ACHY03_000360"
     CDS             complement(28607..29014)
                     /locus_tag="ACHY03_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFS3903640.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIGFSPRGWSDFCPLKEGALCQLP"
     gene            complement(29011..29574)
                     /locus_tag="ACHY03_000361"
     CDS             complement(29011..29574)
                     /locus_tag="ACHY03_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFS3903641.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(29565..30035)
                     /locus_tag="ACHY03_000362"
     CDS             complement(29565..30035)
                     /locus_tag="ACHY03_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFS3903642.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30220..31014)
                     /gene="thyA"
                     /locus_tag="ACHY03_000363"
     CDS             complement(30220..31014)
                     /gene="thyA"
                     /locus_tag="ACHY03_000363"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFS3903643.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(31021..31896)
                     /gene="lgt"
                     /locus_tag="ACHY03_000364"
     CDS             complement(31021..31896)
                     /gene="lgt"
                     /locus_tag="ACHY03_000364"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFS3903644.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(32047..34293)
                     /gene="ptsP"
                     /locus_tag="ACHY03_000365"
     CDS             complement(32047..34293)
                     /gene="ptsP"
                     /locus_tag="ACHY03_000365"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS3903645.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34306..34836)
                     /gene="rppH"
                     /locus_tag="ACHY03_000366"
     CDS             complement(34306..34836)
                     /gene="rppH"
                     /locus_tag="ACHY03_000366"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFS3903646.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(35189..35335)
                     /gene="ygdT"
                     /locus_tag="ACHY03_000367"
     CDS             complement(35189..35335)
                     /gene="ygdT"
                     /locus_tag="ACHY03_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFS3903647.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            35521..36210
                     /gene="mutH"
                     /locus_tag="ACHY03_000368"
     CDS             35521..36210
                     /gene="mutH"
                     /locus_tag="ACHY03_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFS3903648.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            36279..36992
                     /locus_tag="ACHY03_000369"
     CDS             36279..36992
                     /locus_tag="ACHY03_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFS3903649.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            37130..37348
                     /gene="ygdR"
                     /locus_tag="ACHY03_000370"
     CDS             37130..37348
                     /gene="ygdR"
                     /locus_tag="ACHY03_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFS3903650.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            37456..38496
                     /locus_tag="ACHY03_000371"
     CDS             37456..38496
                     /locus_tag="ACHY03_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFS3903651.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(38529..39722)
                     /gene="lplT"
                     /locus_tag="ACHY03_000372"
     CDS             complement(38529..39722)
                     /gene="lplT"
                     /locus_tag="ACHY03_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFS3903652.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(39715..41874)
                     /gene="aas"
                     /locus_tag="ACHY03_000373"
     CDS             complement(39715..41874)
                     /gene="aas"
                     /locus_tag="ACHY03_000373"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFS3903653.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            42460..43491
                     /gene="galR"
                     /locus_tag="ACHY03_000374"
     CDS             42460..43491
                     /gene="galR"
                     /locus_tag="ACHY03_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFS3903654.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNHPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(43498..44760)
                     /gene="lysA"
                     /locus_tag="ACHY03_000375"
     CDS             complement(43498..44760)
                     /gene="lysA"
                     /locus_tag="ACHY03_000375"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFS3903655.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSALQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAARQQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            44882..45817
                     /gene="lysR"
                     /locus_tag="ACHY03_000376"
     CDS             44882..45817
                     /gene="lysR"
                     /locus_tag="ACHY03_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFS3903656.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFHQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAIKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(45804..46496)
                     /gene="ygeA"
                     /locus_tag="ACHY03_000377"
     CDS             complement(45804..46496)
                     /gene="ygeA"
                     /locus_tag="ACHY03_000377"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFS3903657.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FQEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(46625..48043)
                     /gene="araE"
                     /locus_tag="ACHY03_000378"
     CDS             complement(46625..48043)
                     /gene="araE"
                     /locus_tag="ACHY03_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFS3903658.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(48358..49119)
                     /gene="kduD"
                     /locus_tag="ACHY03_000379"
     CDS             complement(48358..49119)
                     /gene="kduD"
                     /locus_tag="ACHY03_000379"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFS3903659.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(49149..49985)
                     /gene="kduI"
                     /locus_tag="ACHY03_000380"
     CDS             complement(49149..49985)
                     /gene="kduI"
                     /locus_tag="ACHY03_000380"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFS3903660.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVID
                     LR"
     gene            complement(50272..51453)
                     /locus_tag="ACHY03_000381"
     CDS             complement(50272..51453)
                     /locus_tag="ACHY03_000381"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFS3903661.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVIDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLAHIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            51708..52937
                     /locus_tag="ACHY03_000382"
     CDS             51708..52937
                     /locus_tag="ACHY03_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3903662.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGIKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT        13200 a        14345 c        13020 g        12727 t
ORIGIN      
        1 cacctaagcc aggaatgccc gttttcggca taaacaatta atacggtcat ctggtttgtg
       61 ttttttatga tttattttct gaaacgggca tgaaatttcg attattaaag tgatggtagt
      121 cacataaagt caccttccag ctaataagtg tgaccgccgt catattacag agcgtttttt
      181 atttgaaaat gaatccatga gttcatttca gacaggcaaa tattcactga tatgaagccc
      241 gaactcgctg gttttgcact tttgaaaaca taaccgatta cgtgcttaag cttctgaacc
      301 taagaggatg ctatgggaaa cacatcaata caaacgcaga gttaccgtgc ggtagataaa
      361 gatgcagggc aaagcagaag ttacattatt ccattcgcgc tgctgtgctc actgtttttt
      421 ctttgggcgg tagccaataa ccttaacgac attttattac ctcaattcca gcaggctttt
      481 acgctgacaa atttccaggc tggcctgatc caatcggcct tttactttgg ttatttcatt
      541 atcccaatcc ctgctgggat attgatgaaa aaactcagtt ataaagcagg gattattacc
      601 gggttatttt tatatgcctt gggtgctgca ttattctggc ccgccgcaga aataatgaac
      661 tacaccttat ttttagttgg cctatttatt attgcagccg gattaggttg tctggaaact
      721 gccgcaaacc cttttgttac ggtattaggg ccggaaagta gtggtcactt ccgcttaaat
      781 cttgcgcaaa catttaactc gtttggcgca attatcgcgg ttgtctttgg gcaaagtctt
      841 attttgtcta acgtgccaca tcaatcgcaa gacgttctcg ataaaatgtc tccagagcaa
      901 ttgagtgcgt ataaacacag cctggtatta tcggtacaga caccttatat gatcatcgtg
      961 gctatcgtgt tactggtcgc cctgctgatc atgctgacga aattcccggc attgcagagt
     1021 gataatcaca gtgacgccaa acaaggatcg ttctccgcat cgctttctcg cctggcgcgt
     1081 attcgccact ggcgctgggc ggtattagcg caattctgct atgtcggcgc acaaacggcc
     1141 tgctggagct atttgattcg ctacgctgta gaagaaattc caggtatgac tgcaggcttt
     1201 gccgctaact atttaaccgg aaccatggtg tgcttcttta ttggtcgttt caccggtacc
     1261 tggctcatca gtcgcttcgc accacacaaa gtcctggccg cctacgcatt aatcgctatg
     1321 gcactgtgcc tgatctcagc cttcgctggc ggtcatgtgg gcttaatagc cctgacttta
     1381 tgcagcgcct ttatgtcgat tcagtaccca acaatcttct cgctgggcat taagaatctc
     1441 ggccaggaca ccaaatacgg ttcgtccttc atcgttatga ccatcattgg cggcggtatt
     1501 gtcactccgg tcatgggttt tgtcagtgac gcggcgggca acatccccac tgctgaactg
     1561 atccccgcac tctgcttcgc ggtcatcttt atctttgccc gtttccgttc tcaaacggca
     1621 actaactgaa catattttcc gaataaagtg aggaatctgt aatgaaaaaa atcagcttac
     1681 cgaaaattgg tatccgcccg gttattgacg gtcgtcgcat gggtgttcgt gagtcgcttg
     1741 aagaacaaac aatgaatatg gcgaaagcta cggccgcact gctgaccgag aaactgcgcc
     1801 atgcctgcgg agctgccgtc gagtgtgtca tttccgatac ctgtatcgcg ggtatggctg
     1861 aagccgctgc ttgcgaagaa aaattcagca gtcagaatgt aggcctcacc attacggtaa
     1921 cgccttgctg gtgctatggc agtgaaacca tcgacatgga tccaacccgc ccgaaagcca
     1981 tttggggctt taacggcact gaacgtcccg gcgctgttta cctggcagcg gctctggcag
     2041 ctcacagcca gaaaggcatc ccggcattct ccatttacgg tcatgacgtt caggatgccg
     2101 atgacacatc gattcctgcc gatgttgaag aaaaactgct gcgctttgcc cgcgccggtt
     2161 tggccgtcgc cagcatgaaa ggtaaaagct atctgtcgct gggcggcgtt tcgatgggta
     2221 tcgccggttc tattgttgat cacaacttct ttgaatcctg gctgggaatg aaagtccagg
     2281 cggtggatat gaccgaactg cgtcgccgta tcgatcagaa gatttacgac gaagccgaac
     2341 tggaaatggc actggcctgg gctgataaaa acttccgcta tggcgaagat gaaaataaca
     2401 aacagtatca acgcaatgcc gagcaaagcc gcgcagttct gcgcgaaagt ttactgatgg
     2461 cgatgtgtat ccgcgacatg atgcaaggca acagcaaact ggccgatatt ggtcgcgtgg
     2521 aagaatcact tggctacaac gccatcgctg cgggcttcca gggacaacgc cactggaccg
     2581 atcaatatcc caatggcgac accgccgaag cgatccttaa cagttcattt gactggaatg
     2641 gcgtgcgcga accctttgtc gtggcgaccg aaaacgacag tcttaacggc gtggcaatgc
     2701 taatgggtca ccagctcacc ggcaccgctc aggtatttgc cgatgtgcgt acctactggt
     2761 caccagaagc aattgagcgt gtaacggggc ataaactgga tggactggca gaacacggca
     2821 tcatccattt gatcaactcc ggttctgctg cgctggacgg ttcctgtaaa caacgcgaca
     2881 gcgaaggtaa cccgacgatg aagccacact gggaaatctc tcagcaagag gctgacgctt
     2941 gcctcgccgc taccgaatgg tgcccggcga tccacgaata cttccgtggc ggcggttact
     3001 cttcccgctt ccttaccgaa ggcggcgtcc cgttcaccat gactcgtgtc aacatcatca
     3061 aaggcctggg accggtactg caaatcgcgg aaggctggag cgtggaattg ccgaaggatg
     3121 tgcatgacat cctcaacaaa cgcaccaact caacctggcc aaccacctgg tttgcaccgc
     3181 gcctcaccgg taaagggccg tttacggatg tgtactcggt aatggcgaac tggggcgcta
     3241 accatggggt tctgaccatc ggccacgttg gcgcagactt tatcactctc gcctccatgc
     3301 tgcgtatccc ggtatgtatg cacaacgttg aagagaccaa agtgtatcgt ccttctgcct
     3361 gggctgcgca cggcatggat attgaaggcc aggattaccg cgcttgccag aactacggtc
     3421 cgttgtacaa gcgttaatac attactccct gatgtgatgc ccggtcgctc cggctaccgg
     3481 gcctgaacaa gcaagagtgg ttagccggat aagcaatgtt atccggctat attgcaggag
     3541 cgattatgaa acaagaagtt atcctggtac tcgactgtgg cgcgaccaat gtcagggcca
     3601 tcgcggttaa tcggcaggga aaaattgttg cccgcgcctc aacgcctaat gccagcgata
     3661 tcgcgatgga aaacaacacc tggcaccagt ggtctttaga cgccattttg caacgctttg
     3721 ctgattgctg tcggcaaatc aatagtgaac tgactgaatg ccacatccgc ggtatcgccg
     3781 tcaccacctt tggtgtggat ggcgctctgg tagataagca aggcaatctg ctctatccga
     3841 tcattagctg gaaatgtccg cgaacagcag cggttatgga caatattgaa cggttaatct
     3901 ccgcacagcg gttgcaggct atttctggcg tcggagcctt tagtttcaat acgttatata
     3961 agttggtgtg gttgaaagaa aatcatccac aactgctgga acgcgcgcac gcctggctct
     4021 ttatttcgtc gctgattaac caccgtttaa ccggcgaatt cactactgat atcacgatgg
     4081 ccggaaccag ccagatgctg gatatccagc aacgcgattt cagtccacaa attttacaag
     4141 ccaccggtat tccacgccga ctcttccctc gtctggtgga agcgggtgaa cagattggta
     4201 cgctacagaa cagcgccgca gcaatgctcg gcttacccgt tggcataccg gtgatttccg
     4261 caggtcacga tacccagttc gccctttttg gcgctggtgc tgaacaaaat gaacccgtgc
     4321 tctcttccgg tacatgggaa attttaatgg tccgtagcgc ccaggttgat acttcgctgt
     4381 taagtcagta cgccggttcc acctgcgaac tggatagcca ggcagggttg tataacccag
     4441 gtatgcaatg gctggcatcc ggcgtgctgg aatgggtgag aaaactgttc tggacggctg
     4501 aaacaccctg gcaaatgttg attgaagaag ctcgtctgat cgcgcctggc gcggatggcg
     4561 taaaaatgca gtgtgattta ttgtcgtgtc agaacgctgg ctggcaagga gtgacgctta
     4621 ataccacgcg tgggcatttc tatcgcgcgg cgctggaagg gttaactgcg caattacagc
     4681 gcaatctaca gatgctggaa aaaatcgggc actttaaagc ctctgaatta ttgttagtcg
     4741 gtggaggaag tcgcaacaca ttgtggaatc agattaaagc caatatgctt gatattccgg
     4801 taaaagttct cgacgatgcc gaaacgaccg tcgcaggagc tgcgctgttc ggttggtatg
     4861 gcgtagggga atttaacagc ccggaagaag cccgtgcaca gattcattat cagtaccgtt
     4921 atttctaccc gcaaactgaa cctgaattta tagaggaagt gtgaaatgct gaaaacaatt
     4981 tcgccgttaa tttctcccga actattgaaa gtgctggcag agatgggaca tggagatgaa
     5041 attatttttt ccgatgctca ctttcccgcc cattcgatgg gaccgcaagt gatccgcgct
     5101 gatggcctgt tggtgagcga cttgctccag gcgattatcc cgttatttga gctggacagt
     5161 tatgcaccgc cgctggtgat gatggcagcg gtagaaggtg acactctcga tcctgaagta
     5221 gaacgacgtt accgtaatgc gctttcacta caagccccgt gtcctgacat catccgcatc
     5281 aatcgttttg cgttttatga acgggcgcaa aaagcctttg cgatcgttat cacaggcgaa
     5341 cgagcgaagt acgggaatat tcttttaaaa aaaggggtaa caccgtaatc tcataccggt
     5401 acgcccgcat gacgcgggcg gttatcgaat gatggggtga aaaatatgaa agcggcacgc
     5461 cagcaagcga tagtcgacct gctgctgaac cataccagcc tgaccacgga agctctctct
     5521 gaacagctaa aggtcagtaa agaaaccatt cgtcgcgatc tcaatgaatt acagacgcag
     5581 ggtaaaattc tgcgcaatca tggacgcgct aaatatatcc accgtcaaaa tcaagacagt
     5641 ggcgatccct ttcacatcag gctgaaaagc cattatgcgc ataaagcaga tatcgcgcgc
     5701 gaggcgctcg cgtggattga agaagggatg gtgatagcct tagacgccag ttcaacttgc
     5761 tggtatctgg cacgccagtt gcctgacatc aacattcagg tcttcaccaa tagccatccg
     5821 atttgccatg aactcggtaa acgcgaacgc attcaactga tcagttccgg cggcacactt
     5881 gagcgcaaat atggctgtta cgtcaatccc tcgctgattt cccaacttaa atcgctggaa
     5941 atcgatctgt ttattttttc ttgtgaaggg atcgatagca gcggcgcact gtgggactcc
     6001 aatgccatca acgctgatta caaatcaatg ctattaaaac gcgccgcgca atcgttgtta
     6061 ttgattgata aaagtaaatt taatcgttca ggggaagccc gcatcgggca tctggatgag
     6121 gtaacgcaca ttatttctga tgagcgccag gttgcaactt atttggtaac agcctgacgg
     6181 aaagggtagc aggccggaaa caacgccccg gccttgcctg ttatcgctcg tcgcgacgac
     6241 cacccaccgc agcccagatg cggcggacgt gcaccgtcac ttcttcgcga tcgtgataca
     6301 actgccgtgc ctgaatctga gcatttatgc catgttcatc aagctgtgcc tgaatatacg
     6361 ccagattgtg tgacacttct tcgtagcgtt ttttcatcgg cagtttgagg ttgaaaatgg
     6421 tttcacgaca ccagccatta accagccact gcgccatcaa tgccgcaact ttcgccggtt
     6481 tttcaaccat atcgcatacc atccaggaga tattgctgcg cgtcggacgg aatttgaaac
     6541 cgtcttcccg cagccacgtc acctgtccgg tatccatcag actttgcgcc atcgggccgt
     6601 tgtcgacgga ataaacccac atgttgcgct tcaccagttg gtaggtccag ccgcctgggc
     6661 aagcgcccaa atccaccgcc cacatcccgt tcgccaagcg ttcatcccac tcatccgcag
     6721 gaataaacac atgaaatgcc tcttccagtt tgagcgtgga acgactcggc gcatctgccg
     6781 gaaatttcag gcgcggaatg cccatataga acggcgaatt attgttgctg tatgagtaac
     6841 cggtataaca gcagcctggt gcaatgaaga atacatgcac taccggacgc ttcggcgttt
     6901 catagttcgc cagcaccccc gcatcgcgca aggcagcgcg tagcggcacg gtgaatttac
     6961 ggcagaattt cagcaactct ttgctttcgt tggtatcggc aacttcaaca cgcagttcac
     7021 cgcccttctc tactacgccc tgtaacatgc cgacaatggg ggtaatacga tcttctggcg
     7081 gcaaatgctg caagagttcc cccaccacaa accactggcg ggcaaaaatt aatgaactga
     7141 acggcagctc acggattaac ttatcgccat catcaggttg ataacattca taaatgacat
     7201 aacccgcatt ctctttcacg cgggcaaaac cgaaaatttc ccgctggccg gctttatcgg
     7261 taatttctgc ggcgcactct ttttcaaagc ccggacggca cagcaataca accttattca
     7321 tgacttacgc ccttgcgctt taaacggata gcaccaacta acattaacgc ccagcccgca
     7381 aggaagctca cgccgccaac cggagtgaca aacgcccaca aacgcagatg ggacagtgcc
     7441 aggcaataaa ggctgccgct gaacagcacc gtgcctaacg cgaggaaaac gctactccag
     7501 taaaaccaga tactgatgcg acgctgcatt gccaccgcca gacctaagat cgccagcgta
     7561 tgaaacgcct ggtattcgag gccggtctgg atccagccca tctcaacggc ccccatggtt
     7621 ttacttaaca catgcgcgcc aaaagcaccc agagccacaa aaatgaagcc gctaatggcg
     7681 gcgaaaatca gcataaaacg gctggtcatg gtcgtaccct acgtaaatta ttgttcataa
     7741 cgaaagcgga atttttcttg ttcagcagcg gctttcgcca ggatccattg gcgaaaggcg
     7801 gctattttac ccagttctgc ctggctgtca tgacaaacca gataaaaagc gtttttactg
     7861 accagaacat cattaaacgg gcaaacaaga cgtccggctt caatttcaga ttgcgccatc
     7921 acgttatttg ccagcgccac tccctgcccg tggatagccg cttgcagcac catggcgcta
     7981 tggctaaaaa ttggcccttg ctgaacgttg atatgattta accccaattg acgagtatat
     8041 gtctgccagt cacggcgtga cgcatcatgt aatagcgtat gtttagccag atcttccggg
     8101 gtcttcaagg gtttttcgcc agtcagcagt agcggcgaac acaccggcaa taaatattcg
     8161 gcgtacagtt tttccacccg tagccccggc cagttgcccc gaccataaaa tatcgccaca
     8221 tcaacatcat ccgccagctt atcttcctga cgatcaaccg cctggattcg aacgtcaatt
     8281 cccggataag ctgaattaaa gctggaaagt cgcggaacca accaatgaat ggcgaaacta
     8341 gggagtaaac tgaccgtcaa cgcccccttg gcgctacggg cctggagttt acgcgtcgct
     8401 tcggttaatt gcgaaaatat ctctttgata tcgaggaaat agctttgccc ttcctcggtc
     8461 agcaggagtg aacgattacg gcggcggaac agttttagcc ccaaaaaatc ctcaagagac
     8521 ttgatttgat gacttactgc ggcctgggtc acaaaaagct cttctgctgc gcgagtgaaa
     8581 cttaaatggc gtgctgcggc atcaaaaact cgtaaggcat ttagcggtgg taatcgttta
     8641 gacatggctt ttaaactttg atgttaaatg aatttaacaa ttagatcaca gtgtgtaacc
     8701 tattagtttt tttaatctga gccattataa attgtccgtt gagcttctac cagcaaatac
     8761 ctatagtggc ggcacttcct gagccggaac gaaaagtttt atcggaatgc gtgttctggt
     8821 gaacttttgg cttacggttg tgatgttgtg ttgttgtgtt tgcaattggt ctgcgattca
     8881 gaccatggta gcaaagctac cttttttcac ttcctgtaca tttaccctgt ctgtccatag
     8941 tgattaatgt agcaccgcct aattgcggtg ctttttttta ccttgcgatc gcgaattact
     9001 gatccagttc gaccatctct ttcacgtcgg tacggttgat ctgctgtttg ttgccattag
     9061 cgtctttata cgaaatcata ccggtatcgt tatcagtctg tggtttgccg tcagagacga
     9121 tggtacgtcc gtcattggtg tgcatcacat agttcgaacc ggaacaggcg ctcagggcaa
     9181 aagtcagcat acaggcagaa ataattgcgg cagtcttttt catcatcttc tccttaaagc
     9241 gattaataaa tcttaatagt attgattaac aggctaaaat taacgcctaa cactattcag
     9301 catatgttac tgacgcggct tcgccaggat atccagataa ttctgatggt tagcactctc
     9361 cttgtatcaa agtgaatttt gcgtcacgat cggtgcatca agccgaggag taccatgaac
     9421 gtttttaatc ccgcgcagtt tcgcgcccag tttcccgctc tacaggatgc gggcgtctat
     9481 ctcgacagcg ccgcgaccgc gcttaaacct gaagccgtgg ttgaagccac ccgacagttt
     9541 tacagtctga gcgccggaaa cgtccatcgc agccagtttg ccgaagccca acgcctgacc
     9601 gcgcgttatg aagctgcacg agagaaagtg gcgcaattac tgaatgcacc ggatgataaa
     9661 actatcgtct ggacgcgcgg caccactgaa tccatcaaca tggtggcaca atgctatgcg
     9721 cgtccgcgtc tgcaaccggg cgatgagatt attgtcagcg tggcagaaca ccacgctaac
     9781 ctcgtcccct ggctgatggt tgcccaacaa actggagcca aagtggtgaa attgccgctt
     9841 aatgcgcagc gactgccgga tgtcgatttg ttgccagaac tgattactcc ccgtagtcgg
     9901 attctggcgt tgggtcagat gtcgaacgtt actggcggtt gcccggatct ggcgcgagcg
     9961 attacctttg ctcattcagc cgggatggtg gtgatggttg atggtgctca gggggcggtg
    10021 catttccccg cggatgttca gcaactggat atcgatttct atgctttctc tggtcacaaa
    10081 ctgtatggcc cgacgggtat cggtgtgctg tatggcaaat cagaactgct ggaagcgatg
    10141 tcgccctggc tgggtggcgg caaaatgatt cacgaagtga gttttgacgg cttcacgact
    10201 caatctgcgc cgtggaaact ggaagcagga acgccaaatg tggctggtgt cataggatta
    10261 agcgcggcgc tggaatggct ggcagattac gatatcaacc aggccgaaag ctggagccgt
    10321 agtttagcaa cactggcgga agatgcgctg gcgaaacgtc caggcttccg ttcattccgc
    10381 tgtcaggatt ccagcctact ggcctttgat tttgctggcg ttcatcacag cgatatggtg
    10441 acgctgctgg cggagtacgg tattgccttg cgggccgggc aacattgcgc tcagccgcta
    10501 ctggcagaat taggcgtaac cggcacactg cgcgcctctt ttgcgccata taatacaaag
    10561 agtgatgtgg atgcgctggt gaatgccgtt gaccgcgcgc tggaattatt ggtggattaa
    10621 tgacgaaccc gcaattcgcc ggacatccgt tcggcacaac cgtaaccgca gaaacgttac
    10681 gcaatacctt cgcaccgttg acgcaatggg aagataaata tcgccagttg atcatgctgg
    10741 ggaaacagct tccggctttg ccagacgagt taaaagcgca ggctaaagag attgccggat
    10801 gcgaaaaccg cgtctggctg ggatataccg tggctgaaaa cggtaaaatg cacttctttg
    10861 gcgacagcga aggacgtata gtgcgcggcc tgctggcggt gctgttgact gccgttgagg
    10921 ggaaaaccgc cgccgaattg caggcacagt cgccgctggc attgtttgat gagctgggat
    10981 tgcgtgcgca acttagcgcc tcacgcagcc aggggttaaa tgcgttaagc gaggcgatta
    11041 tcgctgcgac gaagcaggtt taagcacaga accgggtcgg ataagacgtt tgcgtcgcat
    11101 ccgacctgat tgtttacccc tgacgcgccg ctttcgccat catcttcttc agcgcatgag
    11161 aaaccgcaac aaaaccaaag gtggcggtca ccatcgttgc cgcgccaaat ccagatgcac
    11221 aatccatccg ctttggccct tctgccgtgg ctttcatagc acaaaccgta ccatctgact
    11281 gcggatacac tagcgcttca gtagagaaca cgcaatcgac gccgagctta cctttactgt
    11341 ttttcacaac gccaaaatcg cttttcaggc gctcacgcaa cttcgccgcc agcgggtcct
    11401 gaatcgtttt cgccagatcg gtaacctgaa tctgcgttgg gtcaatctgc ccacccgcgc
    11461 caccggtcgt gaccagcggg attttattgc gccgacaata agcaatcagc gccgctttgg
    11521 gccgtacact atcaatggca tcaatcacgt acgaataacc cacgctcata tgctgcgcta
    11581 cgttatccgg cgtcacgaaa tcatccacca ccgttacacg gcactccggg ttaatctggc
    11641 gaatacgctc cgccataact tccgcttttg ccagcccaac gttatcgcgc agggcatgaa
    11701 tttgccgatt ggtattggtg acgcacacat catccatatc gataagcgtg attgcgccaa
    11761 tccccgtgcg cgccagcgct tccgccgccc aggaaccgac accgccaata ccgaccacac
    11821 aaatatgcgc gtcagcaaac agttgcagcg ctttttcacc atacagacgc gctgtgccac
    11881 caaaacgctg acgccatgca tcactaatta ccacagacat agcacctcag aattaaaaag
    11941 ggcgaggtta cccctcaccc taaacgcata aatgcctgat gcgctacgct tatcaggcct
    12001 acataactcc tgcaatattt tggatttgca tgatttgtag gcctgataag gcgttcacgc
    12061 cgcatccggc atgaacaacg cgcacttagt caataatcag gatgtgaggt tatccctcac
    12121 cctgtcatat ccgtaaaaac ggcatacaga atatcacatc agccgctaaa gacgttacct
    12181 gcgcccggag ccgttttcag cacccaaacc cgtccatagt ggttgtacca acctgcgcgg
    12241 tgtccggctt ccggcccgat cccttgatag atatcgaagt gttggccttt gattgcgcca
    12301 ccgacatcca gcgccaccat cagacgcagt tcatactgac cgttaaattt gccgttatta
    12361 tccagcagcg gcacttctgc cagcaaggtt gtacctggcg gaataatgga acgatcagag
    12421 gcaacagaag cacggccaac cagcggcacc gcacttgccc ctttcaccgg tgcaaaagat
    12481 tgcggtttaa agaaaacgaa agacgggttc tgttccagca gctcgcgaac ctcggcttca
    12541 ctgtgcgttt cgccccagtg acgaatcgcc tgcatcgaca tatcttcttt tttcacttcg
    12601 ccacggtcga tcagcacctt accaatgctg cgataggcat gaccgttttt ccctgcatag
    12661 ctgaaaaagt taagcggact gccatcacca aagtcgatat acccactacc ctgcacatcc
    12721 ataatgaagt tatccatcag ggagttactg taagcgagaa tatatttatc actcaatgcc
    12781 cccgcgtaga tctccgcacg agacggcaga cgaccacgtt ttggcggcat acggtaaata
    12841 ggatactgga actcgccctg gcgggtatgg cgcgcctgaa ttaccggcgt gtaatagcct
    12901 gtaaactgca cgttaccata gttgtcggca ccttccatct gccaggcatc aatgccgaac
    12961 tggcgcatat tgcgggtatc accgcctgcg cgcagccact cttgcaccgc gttataaaca
    13021 ttactctggt tgccatacag acgcggtgac gaattacgga tatggttaat ttgctcggca
    13081 aaatcaccgg cgttaatcgg cgcgccaacg gcatctggct ggttcaccag agagaaaggc
    13141 tgggtaaatt tcccgtcttt atattgctgt ccgcgatcgg ttggtttgga agagcaggcg
    13201 gcaagcattg ccacaaccgt gcccataaga aggtacttta cccaacgtcc tttcattgtt
    13261 ctcttcttca ggttaaaaaa taaggcgcaa cgaagataac aaaccaccgc gtttaatgaa
    13321 atgagcagcc tctccctgac gcaaattttg cacaaaaaat aggctttagt gatttgtttt
    13381 tgttcaaaat catgccaaat ccgtgatcgg ggtaaaaaaa aggttgcatg aaaacgcgag
    13441 cggagtatag tgcgcatcca cggacgcggg gtggagcagc ctggtagctc gtcgggctca
    13501 taacccgaag gtcgtcggtt caaatccggc ccccgcaacc aattattgaa caccctaacg
    13561 ggtgtttttt tgtttctggt ctcccataaa aaagcgccat tcagcgcctt tttatcatcc
    13621 ccttctcgcc agcgtcgccc catcggcaaa atacgcttta atccccgcaa gaatagactc
    13681 cgcaacttcc tgctggaaag tcgccgtttt cagtttacgc tcttcctcaa cgttactgat
    13741 aaacgccgtt tcgaccagaa tggagggaat atctggtgcc tttagtacgg caaacccggc
    13801 ctgttcaact tgatttttat gcagcttgtt gattttaccg agcttattca gcactgcttt
    13861 accaaacttc aggctgtcgg caatggtcag cgactgcacc atatcgaaca tggtgtggtc
    13921 gacatagcgg tcaccgcttt tgctcacgcc accaatcaag tccgaggcgt tctgggtttg
    13981 tgccagatat tttgccgcag tactggttgc acctttggtt gagagcgcaa acacagagga
    14041 accgctcggc tgacgactgg taaaggcgtc ggcatggata gagacaaaca ggtcagcacg
    14101 ctgtttctgg gcttttgcta cgcgcacttg caacggaatg aagatgtctt cattgcgcgt
    14161 catgtacacc ttcatattgc cctctttctc gatcagagag cgcaaacggc gagctatttg
    14221 caataccaca tctttttcgc gcgttttgta tttccccacc gcaccggagt cttcgccgcc
    14281 gtggccaggg tcgagcataa tgacaatcgg acgatcgcgc ccggccttgc ctggctgtgg
    14341 gccactctgc gcaggcggca cctgcttttc gaggtcgcct ttattgtaat cttccagcag
    14401 cgccagcaat gggtcctgca tgtcctgagc attggccgga taaaggtcca tcaccagacg
    14461 ttctttaaac ccggcgaccg gcgcgagggc aaacagctgc ggttttacgt tttgctttaa
    14521 ttcaaaaacc atccgtacgg tttgcgggtc aaattgcccg acgcgcgccg acttgatgaa
    14581 cgggtcgtct gcacgaattt gtgccgccat ccccttgagt accgagttca ggtttacatc
    14641 ttcgatatcc accaccacgc gttccgggtt actcaacgcg aactgcttat atttcagctg
    14701 acgatttgat tctaccgtca cgcgggtgta gctggacgca ggccagacgc gcaccgccac
    14761 gacctggctg accgcagcca ggctgacctg acttacgctc aatagccaca tggcacccgc
    14821 gccttgcagt aaacgacgac ggctgattgc agtgttggat cctgacatgc ctctcccgag
    14881 caaaagaaat ctaagctgtg taacaagtaa acgactaatt tgaccggaaa ctttagcgaa
    14941 agacgcataa tctgtcatct aataaacggt aaacattctt tttatattca cggcattact
    15001 gataaaaaag tcgctctcgc ataaaattta cacttgcacc ctgcgaaaaa acagaataaa
    15061 aatacaataa tttcgaataa tcatgcaaag aggtgtgccg tggtaaagga acgtaaaacc
    15121 gagctggtcg agggattccg ccattcggtt ccctatatca atacccaccg gggaaaaacg
    15181 tttgtcatca tgctcggcgg tgaagccatt gagcatgaga atttctccag tatcgttaat
    15241 gatatcgggt tgttgcacag cctcggcatc cgtctggtgg tggtctatgg cgcacgtccg
    15301 cagatcgacg caaatctggc tgcgcatcac cacgaaccgc tgtatcacaa gaatatacgt
    15361 gtgaccgacg ccaaaacact ggaactggtg aagcaggctg cgggaacatt gcaactggat
    15421 attactgctc gcctgtcgat gagtctcaat aacacgccgc tgcagggcgc gcatatcaac
    15481 gtcgtcagtg gcaattttat tattgcccag ccgctgggcg tcgatgacgg cgtggattac
    15541 tgccatagcg ggcgtatccg gcggattgat gaagacgcga tccatcgtca actggacagc
    15601 ggtgcaatag tgctaatggg gccggtcgct gtttcagtca ctggcgagag ctttaatctg
    15661 acctcggaag agattgcaac tcaactggcc atcaaactga aagctgaaaa gatgattggt
    15721 ttttgctctt cccagggcgt cactaatgac gacggtgata ttgtctccga acttttccct
    15781 aacgaagcgc aagcgcgggt agaagcccag gaagagaaag gcgattacaa ctccggtacg
    15841 gtgcgctttt tgcgtggcgc agtgaaagcc tgccgcagcg gcgtgcgtcg ctgtcattta
    15901 atcagttatc aggaagatgg cgcgctgttg caagagttgt tctcacgcga cggtatcggt
    15961 acgcagattg tgatggaaag cgccgagcag attcgtcgcg caacaatcaa cgatatcggc
    16021 ggtattctgg agttgattcg cccgctggag cagcaaggta ttctggtacg ccgttctcgc
    16081 gagcagctgg agatggaaat cgacaaattc accattattc agcgcgataa cacgactatt
    16141 gcctgcgccg cgctctatcc gttcccggaa gagaagattg gggaaatggc ctgtgtggca
    16201 gttcacccgg attaccgcag ctcatcacgg ggcgaggttc tgctggaacg cattgccgct
    16261 caggcgaagc agagcggctt aagcaaattg tttgtgctga ccacgcgcag tattcactgg
    16321 ttccaggaac gtggatttac cccagtggat attgatttac tgcccgagag caaaaagcag
    16381 ttgtacaact accagcgtaa atccaaagtg ttgatggcgg atttggggta agcgacgaaa
    16441 atcgccggat gcggcaggcg taacactcgt gcgtcgcatc cggcaattac gtttattccc
    16501 gcgaactaaa caacgccgcc agaccactgc gccgctcagt acgagtggcg attgccgcac
    16561 ttaatatacg ctcatcggca tacagcgaca gacggcgacg cgcgcgggtc accgcggtgt
    16621 ataccagctc tcgcgttact accggcgtgc gttggctcgg taaaatcaac gccgcatggt
    16681 cgaactccga tccctgcgat ttatgtaccg tcatcgccca cgtagtttcg tgctctggca
    16741 agcgactcgg ttgcacagac ttaatattgc cgtccggcat cgcaaaccag acgcgcgtcc
    16801 cctgcccgcg atccagcgca ataccgatat cgccattaaa caacccaagc gcgctgtcat
    16861 tacgggcaat catcactggt cgaccttcgt accaacgaga gtgcggatga cgatgaattt
    16921 tgcgcttctg ttgcataaac tgctcaattc gctcattcag tccagccacg ccaaacggcc
    16981 cttcccgcag ggcgcacaaa agctggtact cattgaacgc ctgaatgatt aaatccggct
    17041 cggcacgcgc ttgcagcaga tccagataac gtccgtaacc cgcaagagct tcctcaagca
    17101 tcgcaatata atcttcgccg ctctgtaaaa gtcgtttttc gatatcagta aaatcctgct
    17161 gaaaaacggt tttcactgcc gttttatcac cacggttgat cgccgcagct aactgaccaa
    17221 tgccagaatc gctgccgaaa cgatagcttt tttgcagcag gcagagactg tcgcgcaaag
    17281 atgccgcttc tgtgccagtt cctgccggaa cgtgagtccc cgtcaggcgg cttagctgcc
    17341 tggcacgctc ggcggtaaag cccgcgttgg cataagcgca gatatcgccc agcacagccc
    17401 cagcctcaac cgaggccagt tgatcacgat cgccgagaaa gatcactcgc gcatgatctg
    17461 gcaaggcgtc gatcagtctc gacatcatag gcagatcgat cattgacgct tcatctacca
    17521 ccagcacatc aagatgcagc gggttaccgg catgatgacg taaacgctgg ctacccggct
    17581 gcgcgcccag caatcggtgc aaagtgttgg catcttccgg aatgcgtttc ttttgttcat
    17641 cggtcagcgg taactgtcgc aaagccttgc cgagagattc ggttaagcgc gcggcagctt
    17701 tacccgttgg tgcagccaga cggatacggc agcgttcgcc gtcggccatt tgaattaacg
    17761 ctgccagcaa cttcgccacg gtggtcgttt taccggtgcc tgggccgccg gaaatcaccg
    17821 agatccgccg cgtcagcgcc actgccgctg caactttttg ccagttaatt tcatcgctta
    17881 ctggaaaaag tttgtccagg gtttgcgcca gtagagcttc atcaacctcg atggcatgat
    17941 tcacctcatt gaagaagcgc gctaccgtcc gctcgttaca ccacatgcga ttcaaataaa
    18001 gacgatcgcc acagaggatc atcggcgtgg gttcatctcc cctgctgacc gcttgagaag
    18061 ccaacaagca ttcttcccaa ttttgtagct caccgatttc actgacacag gtcgccaaca
    18121 gcggatgcga cgcctcgtta ttttccagtc gtgaaagcgg caaacaaacg tgtccctctc
    18181 cggcatcatg acttaacagt gccgccgcga tggtgacggc aggatgttca tctcccgcca
    18241 cggtcagggc aaattgcaca tccagcgggc gtagctgttt gtgctccaca gcttccagta
    18301 attgcttttg caatttcatt acgcctcctc cagggtcata ccggcaaaca tctcatccat
    18361 cagggcaatc aacccggcgt tgggtcgggt tgtgtaaatc ccctgttgcg gatgttcttt
    18421 atcaacgcca cgcaggaaca gataaataac gccgccaaag tggcgctcat agtcgtaatc
    18481 agcaatgcga tggcgcagat aacgatgcag cgccagggta taaagctgat attgcagatc
    18541 atagcggtgt acctgcattg ccgctgccat agcctgttgg gtgtaagccg aactgtcttc
    18601 acccaaccag ttggatttat agtcgagcag gtaataacgc ccttcgtggc ggaacaccag
    18661 gtcgataaag ccttttaaca tgccacgtac ctgcatgaac tccagcggcg ggcagcctgc
    18721 ggatagcggg tcaaactggc ggattaacgt atcaagctga ctggcgataa gcggttcact
    18781 aatcggcaga taaaactcca tctccacctg tttattgcgg gcggaaagtt gactcaggct
    18841 tacgccggtt tcattgagag gtgcctggag gacagccgtg atccactcgg tcaataccgg
    18901 ttcccactgc gattcaaagc cgccgagttc cagtttttcc cgcacccagt tcgggtcaac
    18961 cggctgggta aaatcgaggt cttcaaacaa actgtgcaag aatgtccccg gtgacgcacc
    19021 gcgcgggaat tgatgcggtg ttaacgtcgg ttcttcaacg acgctgacca cgcctgcggc
    19081 atcaacatcc agccgtggca tcagatcctg agcgataccg tgaccacgct gctgcaaacc
    19141 ggagtagctg gtgacacgcc agttatcgcc gggcaatcgt tgtagcgtcc tcgcattcag
    19201 ttctgcagtt aaaacatcat taacctgcca gggctggtta tcaccagttt gtgccgtttg
    19261 ccaggcaata tcatcatcgc ataacgcttc aatacaggtg cgaagccctg ccgcatcttg
    19321 cggttcccct ttttgcagca aacgcccgag cgcactttgg tggacgtcgg tgtcaccttt
    19381 tttatcgcca cgacggcgca ccagcggtgc aacgccgaga ctgcaatgcc aaaccgaacg
    19441 cgtcagcgcc acgtaaagca aacgcagatc ttccgccaga cgttcggcct ccgcgagatc
    19501 gacactttct ggcgcagcgt taagatccag aacagcctca aacgagtggc gatcgtgata
    19561 aaacgcctga tcctggacgc ggaaattggt aataaatggc aaccagacaa gcggatattc
    19621 cagccctttt gatttatgga tcgtgacaat ctgcaccaga tgtttatcac tttcgaggcg
    19681 catttgttgg ctggaggcat tactgtctgg ctcgaggata tgttgcgata accagcgtac
    19741 cagcgcatgt tcactttcca gctgcgttcc ggcttcttga agcagttcgc tgatatgcaa
    19801 gatatcggta agacgccgct caccgcctgc cgttgccagc aagttttcta caatgttacg
    19861 cgccgacatc agcgcccgca gcatcggcat aacgccacgt ttgcgccaga tttgccgata
    19921 accatcgaac tcttcaacta ccgcatccca cgcatgttcg tcattgttca gcgtttcaat
    19981 atccagcgca ttcagcccca tcattgacgt tgccagcgca ctgcgcaggg tgttctcacg
    20041 ttcgggcgtc atcaccgcct gcaacaacca aagcatttcc tgcgcttcca gagtttcaaa
    20101 aacactgtcg cggttcgaaa ggtaaacgga agggatttcc agcaacgtta aggcatcgcg
    20161 cacctgggcg gcctcctggc ggctgcgcac cagcacactg atgtccgaag cacgcaccgg
    20221 acgcgcttcg tcgccgttca tcagcaacgc ttcgccccgc tgtccggctt gtagccagtc
    20281 gcggatttgc gcagcacata cctgcgccat ggtactttga tagtcgccaa cgccgcagct
    20341 ttcgccttcc atcagccaca ttttcatcgc aggctgcgtt tcacctttaa atacaaaacg
    20401 taacgcctga tttttcccgg ctgatttcac tggaataaac ggtatttcgc gaaacatgaa
    20461 cgcgtcatca gtctggctga aaagcttgtt cacgctgttc accattcctg gtgcggaacg
    20521 ccagttggtg tctaaagtgt agtgggcgtg aacttcgcta cgcgccttca tataagtgaa
    20581 gatatccgca ccccggaatg catatatggc ctgtttcggg tcgccaatca gcaacaatgc
    20641 ggtttccggc tgatggtgcc agatacggcg aaaaattcgg tactgctggg ggtcggtatc
    20701 ctgaaattca tcgatcattg ccaccgggaa tcgcgtacgg atcgccgctg ccaacacctc
    20761 gccgctttcg ctacgcagcg cggaatcgag ccgacttaac atgtcatcaa aacccaattc
    20821 gccacggcgg cgtttctcac gcgctactgt ttcgcggatc tcagccaatg cgcgggtgat
    20881 caccagatcg cggatcgaca atggttctgc aagcagttga tcgatcgcct caaacagtgg
    20941 atgtcgcggg gtttcccccc cggccttcgt gcgatcttct aagaaacgct gggagaattt
    21001 ttccagcgac tccggcaact gataactgtt tgtctcttct tctgcccagg cgctgatctt
    21061 gtcgatccat ttagcctgat tgctacggtt aaacttgcgt cgatcaatac cagaagattc
    21121 gatcagcgca tccagttcac ccactgcgtc gcgccactgc tgttttaccg tatcaatacg
    21181 cgccacaatt tgcgcgtgac gggaagccag cgtttcatca tcgggcggcg gtgctttgat
    21241 aaccggcgct tcgccttgca gataacgatt aatatcgcgc agcaacgcct gcggcccttt
    21301 ccaggtttca aagacgacct gggctatttc acgcggcagc gggtagcagt ggcgacgcca
    21361 gaaatcggcg caggcctggt agcgtagcag agactcatct tcaatcagct gctgctcaaa
    21421 cagcatgccg gattcaaagg cattcaggtt gagcatgcgc tggcaaaagc cgtgaatagt
    21481 aaagactgcc gcttcatcca tctgccgttc ggctaacaac aaccactgcg cggcttgcgc
    21541 tttatcgtcg atctcttcca gcaggcgttc gtacagtgga ttgtcggtgg tttcacgcag
    21601 acaggcgatg cgcaactcgt ggatattgct acggatacga ccgcgcaatt ctgccgtggc
    21661 agcctcggta aaggtgacca ccagcagttc ttcaacggtc agcgggcggg gaaaggcggc
    21721 ggaaccgcct agtccaagta acaggcgcaa atagagcgcc gcaatcgtaa aggttttgcc
    21781 tgtgccggca gaggcttcaa tcaggcgctc gccctgtaag ggcaagcgca aaggatctag
    21841 tgtctcggcg acatcactca ttcttttcac tcatcagggg cattgtttgc tgcaacgcgc
    21901 tgacgttctc ccacactttc cagccttcag gatgcacata ttcggctttc ccgttctggc
    21961 tgccggaaat ctgcgacaga atagccatgc cttgcggctc gaccaccgcc tgatggaaga
    22021 aatcagcaag tttttgcggc gtcagcagtt ttatctgggc cacgatttta tcacgcgaat
    22081 cgaagcgcat attgccgcga tcgaaatctt tacttaactt cgatgcttct tcgccgagcg
    22141 tttgcggtgc ctgcagcatc tgggtaatta ccgcctgctg gatttgcgca aactcatctg
    22201 gcttcatcgc tcgcaatttt gcctctgcgg ttgggaaaaa cgccttgtaa cgctcccaca
    22261 agaatgaagg ctgtttatca ttgctttgca aaaggaagcc catgccccac tgacgcccca
    22321 cgctcattgg aaacgcaaac acggcatagc ccaattgttc ttcggtacgc aactgattgt
    22381 agaaccacgg ctgtacgatc tgccccaaca gagagctata ggctgagctg gtgtattcat
    22441 cgtagccagt cggtacaaat accgctgcca gtgcggagtc ggtgctgtta ccggcttttt
    22501 caaagatgac ggattgtttt ttatcgacca ctacatcttt gtttcgacac cactctgacc
    22561 catcagcgcc caactgtttt tgcacatcgc gtgccagcgt tgttgcctgg gcctcggtca
    22621 tgttgccgat aaccataaac tctggtcgag cccctgattt taaggcgtcg cgataggcca
    22681 gcacctcttt caaagtaatg gagggcaaaa ttttgcgccg ttcatctcgc gagaagtacg
    22741 gcacttgcga gagcatctgc gcgggcataa tcgcctgctc gaacgctttg cccttttctg
    22801 cggaatccat catctggtta taccaggact tcgcttgctc aagctgatct tccgtagcgg
    22861 tatagctaaa gtacccctcg agcaatgcct ggaacagctg cggcaggcgc tgggtgtaac
    22921 cattggcatt aaccataagg ccgttgttag cgttggtgga aaaacttatg ccaccaaccg
    22981 acgcctggtt gcttaactga tcaagcgcca gccctgcgag ataatcattg agcgcaaaca
    23041 tcacctgatt gcgggcgctg tccatggctt tcggattacg caaaatcagg ctgacatcag
    23101 ctttgggttc gctggaaaaa taacggcttg gcgcatacac cacgcgcaga tttgactcat
    23161 caacaatcag ctctggatgg tcatatttct tgtctgactt aatcagcgag aaatcgtcag
    23221 gaatataggg gttaagctcc ggtaaggaga gcgcaatatt ggcagatttt tgctgccagt
    23281 cagcgaaagt ttgttcgctg attttatcga cctgatacgg cgcatcgaca aaataagccg
    23341 ttttattgtg cggctctttc gggctgatat accagatacg cgcattctgc ggcgtcatca
    23401 tcgccagacg ttcttttact gctttagcat cgtaccgatc ggcaatattg actgcatcca
    23461 gcgtatgctc aaccggaacg cgaatcatgg tatctgccag ccattcgacg taatccatat
    23521 cacgggtgat tgacggataa cggaagtcta tatccagcac attcgccagt tcatcgaagt
    23581 attgtttatc gatccctttt tcacgtaaca gattgaggta gctaaaaatg gccgccacaa
    23641 cctgatcgcg attcgccaga cctttatcgg ttaaagacgc agagatcgct aatacaccgc
    23701 tgttgccgtt gacgatagga tcggagttgg cgctaatgcc ctcaactaat ccctgctttt
    23761 gcagccagtc agaaagtgta cctgggctgc gattaccaat cagataggta atcaattcat
    23821 ccgttttact gcggaacttc gctgagttgt tatcgatgcg aaactcaacg cgcagaactt
    23881 tacgcggcag cgctgggacg taatgaataa taatgccctt ttgggcgtcg gtgactaccg
    23941 gcacggtaat ttccggtttt ttgctctctt tgttcggcac gcgaccaaaa gtgtccgccg
    24001 ccatttttgc caactccggc agcggtttat tactgtaaat aaccgccttc atcagattgg
    24061 cggaatagta cttctcgtgg aaatctttca gcgcctgctg caccggatta ccaggtttgt
    24121 cgcttaaagt ttcgaggtta ccaccagaaa actttgaacc ggggtgtgcc gggttaatgg
    24181 tttctgcgct gacttgtgcc atgcgcatcc cgtcacgcgt acgcgccatg gttaattcgg
    24241 cattcaccgc attacgctca cgttcggcgt atttcttgtc gagcaagggt tctgcaatag
    24301 cgtccgccag gcggtctacc gcaccgggca aggcgtcgtt ctcaacttcc agatagaaag
    24361 ccgtgcgata cggtgccgtg ctggcattgt ggctgccacc gtgcattttg agatattcgg
    24421 ccagactgtc agcctgcggg tactttttcg accccatcag actcatatgt tcaaggtaat
    24481 gtgccagccc ctggtacgcc tcgggatctt ccagcgaccc aacgggcacc accagcgccg
    24541 agagcgattt aactgcctgc ggatcagaaa ccagcaagac caccataccg ttatccagac
    24601 gtatagcctg atactggcgg ttatctttat cacttttacg gatggtttcc tgaatcggct
    24661 gccatcccgt ttctgcctga cttaagggtg cccaaagggc aactaacaac aataatgctt
    24721 tgaaccaggt gctgcggggc attcacggac ctcataatca actttatttt ctggccagat
    24781 tcaacaggtt aacgcaaatc atctgccaga atttaatctt gtgctgcacg agtcagccta
    24841 tgtttatata accatcagtc cgtgactggt gcgcatcata aagtaagcgg atggattgcg
    24901 caatttttat acaactctca tgactgatta aagcgaaaca gcggtaacag gaaacgttgc
    24961 gactgttcaa cgattgcctc cattgtctct ggtgttaatt gccgccagag cctttgatac
    25021 cagatatcat caccttcgcc acgcaccatc atgttgcctt cgtaagcctg aaggaatttc
    25081 gtacgggctt tttgcaacgt ggaatcgtca tccagcatgg catcgttttg cgcgtcataa
    25141 caggttttta gccacgcgcc gccactttca ggtaacacca gcaatggcgc ggacatccct
    25201 tcacgatacc cctcaatcag ttgtgagagg taatgcaaag cctgttcggc tgcaagcggc
    25261 ggaaaacgcc actcgccgtc tttgcgtaga aaaaggcgac tttcaccatt accaccgctg
    25321 gcacagtaga caaggtgttc cagccaaagt tgcattccct gcgccacact taataaagag
    25381 ggacgccagc gcaacaagcc atccggctgc acctgcggca accagccagt tatctgcaca
    25441 ccgttgcaag cgagatcaat ttccatactc tgccccggct ggcgacaggc aatgactctg
    25501 ttggcaagct gctgcatctc ctggcactgt gtttcccaga aaatttcacc aaaagcgcca
    25561 tacggtaaat cccctgccgc ccggaagcgg cggaacaagc gttcggcatc atcctgctca
    25621 accagtgcat tcaataactg ctgattgatt tgataacggc taagtccttc cagaataaat
    25681 ggctcggtgt cggggatttc gctgtcttca gtacggaagt tcacctgcaa acgcatctgg
    25741 aaaaacgccc gcaccggatg tgcccagaat cgttgtagcg tttccagcgg cacggtttcc
    25801 ggtaaggtaa acggcagcgg ctgaacaaat tcagaatgtg ctttaccagc ctggctggcc
    25861 gcaggtagcc attcacgagc atagctttgt cgttcgcctg gctggtagtt ttgtggatca
    25921 aacggcatcc gggtatggag gcaagtaaga tgcgctttta cccttgcctc gctttcatca
    25981 cagttgagcg cttcatcgcc cggtagataa tgactttgcc cgatgtagtc gatcagttcc
    26041 tgcaccagta ccgacgggaa acgttcactg ttatcctgaa tggagcgccc gatatagctg
    26101 atataaagtt tttgttgcgc ggaaattaac gcttccagga acagatagcg gtcatcatcg
    26161 cgacggctac ggtcgccacg cttcggtttc tgggtcatca ggtcaaagcc caatggcgca
    26221 agctggcgtg gataaacgcc gtcgttcatt cccagcaggc aaaccacttt gaacggaatt
    26281 gaacgcattg gcatcagagt acaaatgtta accggtccgg cgagaaaacg ctggctgata
    26341 cgttcttgat ccagacgctg tgccagttca tcacgcaata gtgacagcgg caccgcgtcg
    26401 ccatactgcg cacctaaacc ttcggcgata atcgcctgcc attgttgttc gatcagcgtc
    26461 atcgccgctt cggtttccgc atccggcagg aagaaggcgt tgagcatatc gcgacaaacc
    26521 ggcaaccact cttccagcgg acgctcctgt gccagcccgc gacgccagat gtttagctgc
    26581 attagcagtg aagccagatg ccccaccagt tctgcaatta agccgctcga ttcatcataa
    26641 ggtagaaccg attgccactc gccctgcgcg ctctccatcg cgtagcccaa caacatacgc
    26701 gtcaggccaa atcgccaggt gtgttgtccg gtggcgggga gttccagctc gcgaacgttg
    26761 tcgtcatcta tgccccaacg aatgccggac tcgttgaccc actggcgtaa ataacgcagc
    26821 ccttcttcgg tgatgtcaaa ccgcgccgcc agcaccggca catccagcag cgccagcaca
    26881 tcctctgaca caaagcgact gtcaggcagt gataacaggc tgataaacgc ttccagtacc
    26941 ggatgtgact gccgcgcacg acggtcggaa atggcgtaag gcaggtaacg atccgcaggt
    27001 gcgctaccaa acactgcctg aataaacgga ctgtagctgt cgatgtcggc caccatcacg
    27061 atgatgtcgc gcggagtaag tgtcgggtct tcctccagca tcgccagcag gcgatcgtgc
    27121 aaaacttcaa cttcacgctg cgggctatgg caaacgtgga aggtgatact gctatccagt
    27181 ggatcaagcg ggcgtttgtt atcgctacgg gaaaactctt cgatgttcac accagcaacg
    27241 gcgcggtttt ccagttccag aatgtcagac tgaatgttat gcagcaggtt atctggcgtg
    27301 acatcgacaa aggcgtccag ctcctggctg ctctccaggt cagaaaggag ataaatgtag
    27361 tcgcgcccaa gcttacccca tgaagccagc agcgggttgc cgacatcctg ttcaccatcg
    27421 ctgttaaaga gctgcccggc attttcgcta tcgcgaaata gcggcaattc gcgatcttca
    27481 aaactgtgtc gacgctgacg ggtcagcagt ttcgccagat aagcaggatc tttaatatcg
    27541 ccccagtaat aacggcaggg gttggtaaac aggagatgga tttcaatatg tttacccagc
    27601 gcctggagcg cctggagata aacaggcggt aacgcggaaa taccgcatat aaagacgcgc
    27661 gaaggtaacc ccggcgggca ggtcgtcgcg gactccagcg tttcgataaa gcgctgatag
    27721 agattggcgc ggtgccagcg cggttgtccg agttcatgtg tatattccac cagcgccttc
    27781 cacaacggcg cttgccaggc ctgtgcctct cccagccctt caaccaaatg tcctgtttcc
    27841 cactgtgcca gccagtccgg acgatagacc agatactggt caaacaggtc cgccgctttt
    27901 gaggaaagct ggaacagctt tcgcttgtcg ctatcgtcag tcagataatg ccgcaacagg
    27961 gtaaagtctt cgcgatccag caattgcggc agcagagtca tcagtttcca gctcatgctc
    28021 tgtttgttaa aggcgctctc tttggggatc tccggtaaca cccggacgaa catatcccag
    28081 ataaagctcg caggcagcgg aaaatcaatg tttgccgcaa taccaaactt ttgcgacagg
    28141 gtcatttgca gccactgtgc cataccggta ctttgcacca gaatcatctc tggttcgaaa
    28201 ggatcgtcca gccgttcgcg ttcgacaata aactccatca acgcttccag cacgtccaga
    28261 cgattggaat ggtagaccct taacatagcg gctcctgact actgacgatt cgggcaatgc
    28321 aggcgggtca tctcgccttc tctgcccccg ggtgaaacta gcgtaacgct gatgctgaca
    28381 catcccgcct gcgatgtctg cattcggttg acctgccagt tggcaggtgg cgaaatcgcg
    28441 cgcagttgcg tttgctgcca accatgccgc cagagctgtt ggtactggtt tctgctggca
    28501 agactgttca ttaatgttcg ctggaaaccc gataacgcag tgacaatcac caccatcaac
    28561 accatcgcca acattacctc cggcaggcta aagccttgtt gattcttcag ggaagctgac
    28621 ataacgcccc ctctttcagc ggacaaaaat cgctccagcc gcgtggcgaa aaaccaatgt
    28681 ttccatcgat gacttcacct gttcgccaca acgaaacgcc ttcataaccg gcaatcaata
    28741 aggcttcatt atctgccaat aaacgcaaac aaacccgggc atcggttcca gcgtactgcg
    28801 agcactgaac tgctggctgc gtctgccagg agtgcatttt tccccacgcc agcgccgact
    28861 gaacgatggc ctggcggcgc aatgactgac tttccatgct cacgcgagag gcaaaactgc
    28921 gatcctgttg gctcattcct tgtaatagca agctacccaa aaccagcagc atcaggacca
    28981 gagccagtga cgaaacaccc ttttcgcggt tcacaggttg aatcctgtca cgctatagct
    29041 ggcattcacc acggtttgcg gttcagactt actggcagca cgcatattaa ccgtcaacac
    29101 cggcgagaag ccgctgacat cctgacgtac gacctgaaaa gtgtcgataa tgatggcatc
    29161 cggattagtg actttatccc agcccttacc ttcacaggat gtcgcaccgc gtagcgtttc
    29221 cagcacatgc tccttcagac gaaatccaat ctggtcggac tcttttaccg gttcgcgatc
    29281 ccagataccg ttactgttcg catcccactg cacaatgaca cagtcaccct gtccgacaat
    29341 ttccagccct tcgccggtac agatgccatg acaataaccc gccctctgga gatgcttcgc
    29401 gacggtaaat acccgcagcc agatttcatc ttccagtgcc agcttacggg tgctcgttaa
    29461 actttcacgc tgtaacgcag gcagaaagcg tgcagccccc aacaacaata cgctactgat
    29521 cgccatagca atcaacactt ccagcagaga aaaaccttgc tcttttacag gcatccttct
    29581 gtttctcctt gctgacaaag ccggagtctt ccccacggcg aaaccaccag ccaccactcg
    29641 cccgttgagt ttttgaagcg aatatgcccg gcccatgcgg tattgcgcag gccaaagaag
    29701 gcaagcgaag gtgtcaggtc gctcatttcg acttcgggcc agcgtggcac aaagaccaat
    29761 ggtgaactgc catgacaggt attggcccca gcagcggaac tcacaaggca ccataacgtc
    29821 ccctccctga taacgctgat actgtggtcg cggttatgcc agttggcatc ttcacgtaaa
    29881 tagagcaaat agtctcgcgc ctggctggcg gtttgccata gccgttgcga ctgctgccag
    29941 tattgccagc catagagtcc acttgcactt agcatgacca aaatcagcat cgcgaccagc
    30001 gtttcaatca gcgtataacc acgttgtgtt ttcatgccgg cagtatggag caaggagaaa
    30061 aaaagacgag ggccagtttc tatttcttcg gcgcatcttc cggactattt acgccgttgc
    30121 aggacgttgc aaaatttcgg gaaggtgtct cgaagaattt aatggagggt aaaaaaaacc
    30181 gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt
    30241 aatgcccgga tgtggatcgt agccttcaat ctcaaaatct tcgaaacggt agtcgaagat
    30301 ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg
    30361 cagatgagtt tgatccatat gattgctgta caggtgcgtg tcgccccccg tccagacaaa
    30421 atcgcccact tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc
    30481 aatgttaaac ggcaggccga ggaagacgtc acaggagcgc tgataaagct gacaagagag
    30541 tttgccgtct gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc
    30601 cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag
    30661 ctggttcagt accgtagtga tctggtcaat atgacgacca tccggcgttg gccaggcgcg
    30721 ccactgttta ccatacactg gcccgaggtc gccgttttca tcggcccatt cgtcccagat
    30781 ggtgacattg ttttcgtgta gataagcaat gttagtgtcg ccctgcaaaa accacagcag
    30841 ttcatggatg atggaacgca ggtggcaacg tttagttgtc accagcggga atccatcctg
    30901 caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt
    30961 tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc
    31021 tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc
    31081 gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt
    31141 aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat
    31201 caggaacaag cctgagacag ctcccattgg ccgcggttta cgaatataca ggttgaggat
    31261 aataaacagc accacacctt ccaacagcag ttcgtaaagc tgtgatgggt ggcgcggcag
    31321 cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc
    31381 ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca
    31441 caattcaccg ttaataaagt tgcccaaacg cccggcacca agaccaaacg gaatgagtgg
    31501 tgcgataaaa tcagaaacct ggaagaagga acgtttagta cggcgggcga agataatcat
    31561 caccacgata acgccaatca ggccgccgtg gaaggacatg ccgccgtccc agacacggaa
    31621 cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg
    31681 tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt
    31741 ccagccgctg cccggacgat tcgcccgacg tgttgccagc cacattgcaa aaatgaaacc
    31801 caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg
    31861 atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt
    31921 tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc
    31981 gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga
    32041 tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc
    32101 aacctgatgg cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc
    32161 tgcttcggca aaatcaatgc gccgcagcag gtattttacg cgcgctacag aacgtccgtt
    32221 catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc
    32281 catttcaccg cacaaacgga gatcgattcc atgtatttcc gcttcccggg cgatcatcgc
    32341 cagagctcgt aacattgcag gatgaagact gtcataaatg ttcgccaccc gggtattgtt
    32401 acgatcaacg gccaggatgt attgagtcag atcgttggtg ccaacagaga taaaatcgac
    32461 ccgctttgcc agatgcggca gcataaatac cattgacggt acttccagca tgatgccgat
    32521 acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat
    32581 caggcggcga gcttcgtcaa cttcatcgag gcttgtgacc atcggcaaca gaatattcag
    32641 gttgcccgta gcggcgttag cacgcagcat cgcccgcacc tggatcaaga agatctccgg
    32701 ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg
    32761 catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc
    32821 attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg acggaaaacc
    32881 actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacggctgcc
    32941 cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc
    33001 gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc
    33061 ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc
    33121 accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc
    33181 gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc
    33241 gccatctcgc acgacaacac ccactaagcg atcctggggc agctcagcaa gagttgtcgc
    33301 tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc
    33361 gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcgccag cgcgctcttt
    33421 gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac
    33481 cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt
    33541 gtccgaaagc aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgcaccggc
    33601 ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag
    33661 tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg
    33721 taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg
    33781 gattcgcgtc tggcgatatt gcccaaacaa ggcagtcaac tgcgactgag aaagaatagc
    33841 tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa
    33901 ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc
    33961 taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt
    34021 ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc
    34081 atcaaacgca agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag
    34141 gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc
    34201 aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac
    34261 cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg
    34321 tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacgct
    34381 cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac
    34441 cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat
    34501 attgatttct gcatcgccgc tcaccagctg caagagaaac catttttgtt tttggccgat
    34561 acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt
    34621 acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc
    34681 acggtacatc gcctgctctg cggattctcc ggggttaatt ccgccttgcg gaaattgcca
    34741 ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat
    34801 acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaat ataaagcttt
    34861 atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta
    34921 cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg
    34981 tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac
    35041 tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa atattcatta
    35101 tcagtcaatg cattacatgt aatttaagta atgaaaaaga gtaattcgtg acccaggtca
    35161 cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtatttatcc acagaatgtg
    35221 ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa
    35281 atggaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt
    35341 aagatcctgt ttattttcag tgaccagatt tggaaaaccc gttgcagtgt tgcgcaactc
    35401 gattaccggc aacctaaaaa gcaatataaa tcagagaatt gaacaacgca tgtggaaaaa
    35461 gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac
    35521 atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa
    35581 gcacagcaac tttctggtta tacattggga gaactggcgg cacttgccgg gctggttacg
    35641 ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt
    35701 gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact
    35761 atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgccccgtta
    35821 acgggcaata gcggagtgac ctgggaaacc agccacgtgc gccacaaact caaacgcgta
    35881 ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgttggatca
    35941 ccgttgctgt ggagcccgaa tgaagaggaa gaccggcagc tacgcgaaga ctgggaagaa
    36001 ttaatggata tgattgttct cggtcaggtt gagcggatta ccgctcgtca cggggagtat
    36061 ttacagatac gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg
    36121 ggcgaacgga ttctgacgct gccgcgcggc ttttatttga agaaaaattt caccagtgcg
    36181 ctgctggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca
    36241 tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg
    36301 atcctaacgc ctggcttgcg ctcggtacgc tgacgctgct agagatcgtt cttgggatcg
    36361 acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg
    36421 cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatcg
    36481 cctgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc
    36541 gtgacttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc
    36601 acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg
    36661 ctatcgtgca gattatgctg ctggatatta tcttcagcct cgattcagtg attaccgctg
    36721 tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga
    36781 tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc
    36841 tggctctctc tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc
    36901 acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca
    36961 acctgattcg caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc
    37021 gtactcactc ctgttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc
    37081 ggtttataaa cagactatta tcataggtgg gccaaatgag gataaccgga tgaaaaaatg
    37141 ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta
    37201 cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga
    37261 tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga
    37321 tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga
    37381 tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat
    37441 aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg
    37501 ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa
    37561 ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta
    37621 cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa
    37681 catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat
    37741 gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat
    37801 gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccacag
    37861 cgcccaacca actgcttcgg caaactcggt tatagctgga cagattctgc gcctgcggtt
    37921 tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat
    37981 atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa
    38041 cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt
    38101 gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc
    38161 ctgggtttcg gcacgctgac cgggaaatat ctcaacggtg caaaacccgc tggcgcacgt
    38221 aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg
    38281 gcgtatgttg atatcgccag acgtcatggc ctggatcctg ctcagatggc gctcgctttt
    38341 gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggagcagctg
    38401 aaaactaacg tcgaaagttt acatctggag ttaagcgaag acgtattagc tgaaattgaa
    38461 gcggtacatc aggtttatac ttatccggca ccataaagat taacgccggt taaaaccggc
    38521 gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag cgcaaacagc
    38581 gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc cagcgagtaa
    38641 atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc
    38701 cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg aacgacaaaa
    38761 aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcaacag ctcgtgttgc
    38821 agggagaaaa tcagtaccac cacgccaatc aaaatcccgg ctggcataca gcgtgacacg
    38881 gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc aatcgctacc
    38941 atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac cggtacccac
    39001 agcaccaaca ggaaacgcag cgttacaccc gctccccaga ataaactggt gcccaccagc
    39061 gaaaaacgcg tttcaccatt gcgccatagc gaggtgcagg cattcagaaa actgcgggtc
    39121 atgttgatga gattccagga ctgccccgga cgcgccgccg ccagtttggg aatgtagata
    39181 ttggcaacga ccgcaccacc gtaggccagt gcgcatgcgg ccagggcaac caggacatgc
    39241 cagtcagcca gcacaccacc ggctacggaa ccgagcaaaa tcgccgctat ggtagaagct
    39301 tccattaaac cgttagcttt tactaactta ctacccgtgg ttaattcgcc gagaataccg
    39361 tatttcgccg gtgaataggc tgcagcacca acacccacca gcgtatagcc gagaaacgga
    39421 ttgataccaa agcagatact ggctgcgccc agcagcttca gaccgttggc aaacatcatc
    39481 acccggcctt tggcgaagct atccgccacc tgcccgacaa acggcgcaaa aagaatgtaa
    39541 gcacctacaa acaccatttg caggatgggc tggctccact ccggatagaa ctgcgctttc
    39601 agtaacgcca gagtggcaaa cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc
    39661 acgataaccg ctttcatccc cttcgaccac aacgaagtgt tagtgtgcac tgactcactc
    39721 atcgtgttgt tccgcttcgt ctacccagct tttcaacgtg acaaagtcag gtttgccgct
    39781 gccaagtaat ggcatctgtt tcagatagcg aatatcacgc ggtacagcaa gctccggcac
    39841 gccgtgctcg cgggcatact gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa
    39901 aagcaccagt gcctcgcctt tgctggcatc gctcttaatc gcagtggcat ggactttatc
    39961 tggcgaaaca ccaagtgcca gttgttccac catttccaac gacaccattt cgcctgcaat
    40021 tttggcaaag cgttttgcgc ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac
    40081 gatatcgcca gtgtcatacc agccgcgctc catttcgccg cgaacattct cggcggtggg
    40141 cacttccagt acaccaggct tctccacccg cagatagccg ttcattatgt tcggcccttt
    40201 cagttgcagg cgtccgccct cttcgatacc agggaccgac aacaggcgcg catccatacc
    40261 gggtagaata cgccctaccg taccgggttt cgccgccatc ggtacgttga tagaaacgac
    40321 aggcgcgcat tcggtcacac cgtagccttc aaggatgcgc aggccaaatt tatcctgcca
    40381 aagctgtttg gtactttctt gtagtttttc tgcgcctgcc accacatagc gtagacgata
    40441 gaagtcatac gggttggcaa agcgcgcgta gtgaccgagg aaagtcgagg taccgaacaa
    40501 cacggtgcaa ctgcggtcat acaccaactc cggcacaatg cggtaatgca gcgggcttgg
    40561 ataaaggaac acttctgcac ctgtgagcag tggcgtaaac aggcctaccg tcagcccaaa
    40621 ggagtgaaac agcggcaacg ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt
    40681 tttaatctgc tcgacattcg ccagaatact tttatggcta tggacaacgc ctttcggatg
    40741 gccttcagaa ccggaggtaa agaggatcag cgcctcttct tccggctgct gttttacctg
    40801 tgccagacgc ggcatcagca aatgagcgaa gatccatact ttgtcggcag tggtgacatc
    40861 tgcttttaaa tcttccagat agacccagcg cacctgagta agttgctccg gcagatgcca
    40921 gagtttgcct ttatcgagaa actggcggga agtgaagatg gttttgattt cagccgccgt
    40981 aatggcgctg gtcagccctt ttaccccagc ggtgtagttc atcattgcgg gaatgcggcg
    41041 acgggcgatg gccccaaaaa tcactgccgc actgatgcct gcattgggca gcattaagcc
    41101 gatgcgttcg ccttcaacac tgtatttttc aaggatgcgt ccaacaaaca gcgttttagt
    41161 aagcaatttg cgataggagt ctggggtaaa gttgacgtct tcgacacatt tcttcccggc
    41221 tccgaagcgg tacattgcac tcagtaaaga ttcgtacagc gtttcacgcg ggcgcaccgc
    41281 catacgcgct tccatcatta tttgatgcag catttcgcca gcgattttgc gacggtcacg
    41341 ggcacgcggc gcatccggca tctccacctg cgttggtggc aaaatatgca gagtaatttg
    41401 cgggaacaag cgacgtttaa ccagaccttt caggcggctg aagtgcgtaa gttccgcccc
    41461 ttcaatacgc acaggaataa ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc
    41521 gtagattttc atcagcgagc ctgtggtggt gatgcgtcct tcagggaaaa tcaccactgg
    41581 tcggccctgt tccaccagac gtaccagatg tttaatcgcc ataggttgcg tcgggtcgag
    41641 aggaacaaag tcgataaatg atttcagcca acgcatatac cactgttgac ttattgaggt
    41701 gtaaacggca aataccggac gtacgggtaa aaacaatgcc agcagtatgc catcaataaa
    41761 agagacgtga ttaggcgtga ttagaacgcg ctcgcccttc aatgcctggg tgtcacccgt
    41821 aacgcgaacg cgatacaaaa cacggcacaa atttcgaaaa aagctaaaaa gcattccaac
    41881 tccctttgct ctgattcagt aaaagcgaat ggagggagat tacacgagat aaagaacgcg
    41941 agcgacagta aattaggtgc gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga
    42001 cagggtacga agagcgtacc gaataatctc accaatcaat acctctggga tcttgattgt
    42061 ggtctgcacg acgactcttc gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg
    42121 aggtgtgtaa attgtcggtt actgttacag attgatgacc ggcaaaaaaa aacctgcgca
    42181 tctgcgcagg ctggtgtaat tcatgtgctc aacccgaagt tgacttcacc tatcaatacc
    42241 tctgggatca ccactttagc aacctgaagc caaacgccac cagcggtcaa tcgcaacagc
    42301 gtttcgcaaa gtgtaaccaa aggtttgtaa tctctttttt tgtcttactt atttgcgttt
    42361 ggctcacatt cccacgatga aaacacgcca ccccttgaac caacgggcgt tttccgtaac
    42421 actgaaagaa tgtaagcgtt tacccactaa ggtattttca tggcgaccat aaaggatgta
    42481 gcccgactgg caggcgtttc agtcgccacc gtttcccgcg tcattaataa ttcacccaaa
    42541 gccagcgaag cgtcccggct ggctgtgcat agtgcaatgg agtctcttag ctatcacccg
    42601 aacgccaacg cccgtgcgct ggcgcagcag accactgaaa cagtcggtct ggtcgttggt
    42661 gatgtttccg atccgttttt cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac
    42721 accggtaatt tcttattgat tggcaacggt taccacaacg aacaaaaaga gcgtcaggct
    42781 attgagcaac tgatccgcca tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg
    42841 gatgccgatt tagcctcatt aatgaaacaa atgcccggta tggtgctgat caaccgtatc
    42901 ctgcctggct ttgaaaaccg ttgtattgct ctggacgatc gttacggtgc ctggctggca
    42961 acgcgtcatt taattcagca aggtcatacc cgcattggtt atctgtgctc taaccactct
    43021 atttctgacg ccgaagatcg tctgcaaggg tattacgatg cccttgctga aagtggtatt
    43081 gcggccaatg accggctcgt gacatttggc gaaccagacg aaagcggcgg cgaacaggca
    43141 atgaccgagc ttttgggacg aggaagaaat ttcactgcgg tagcctgtta taacgattca
    43201 atggcggcgg gtgcgatggg cgtgctcaat gataatggta ttgatgtacc aggtgagatt
    43261 tcgttaattg gctttgatga tgtgctggtg tcacgctatg tgcgaccgcg cctgaccacc
    43321 gtgcgttatc caatcgtgac gatggcgacc caggctgccg aactggcttt ggcgctggcg
    43381 gataatcacc ctctcccgga aatcactaat gtatttagtc cgacgctggt acgtcgtcat
    43441 tcagtgtcaa ctccgtcgct ggaggcaagt catcatgcaa ccagcgacta accgcagtta
    43501 aagcaattcc agcgccagta attcttcgat ggtctggcga cggcgaatca accgcgcctg
    43561 accattatca aacagaactt ctggtaacag cggacggctg ttgtagttgg atgacattga
    43621 tgcgccatat gcccccgtat catgcagtac cagataatcc cccgccttca cttccggcaa
    43681 ggcgcgggtt tcaacatttc ccccttcctg ctgggtaaag acatcgcccg attcacataa
    43741 tggcccggcg acgacggttt ccaccgttgg cgcgtgttcc agagaacgac catcagctgc
    43801 cagggcactg atatggtggt aactaccgta cattgccggg cgcatcagat cgttgaaccc
    43861 ggcatcaacc agcacaaagt ggcggctacc catttgtttg acgctccgca cctgagtaat
    43921 taacacgcca gactgcgcta ccagaaagcg acccggttca atttccagtt tcacagggtg
    43981 gcccaaatgg cgggcgattt gctgacgcgc ggcattccac agaccataat aatgctcggt
    44041 atcaaccgcc tcttcaccct gttgataagg aatagaaagc ccaccgcctg cagaaatagc
    44101 ctgtaaatcc tgaccgaatt cgatgacctg acgtaccata gcaccacaaa cttgttccag
    44161 atgggcataa tcaacgccag aaccaatgtg catgtgaatg ccgaccagct gcagatgatg
    44221 acgttgtatc acgtccagtg cggcgggcag atcggtgtac cagataccgt gcttgctgtt
    44281 ttcgccaccg gtattggttt tttggctatg tccgtgacca aaccccggat taacgcgcag
    44341 ccatacccga tgccctggcg aaacctggcc cagttggtcg agcatatcaa cagaacccgc
    44401 attcaccgga atttgcaatg cactgacgcg ttcaagcgtc gcctgatcga taacatctgc
    44461 cgtaaaaaca atatcatcgg ggtgcgtttg cggattgtaa cccgccgcca acgcgcgctc
    44521 tatttcgcct aacgagacgg aatccacttt cacgccctgc tcacgcatta agcgcaaaat
    44581 atgaatattg gaacaggctt tctgtgcaaa gcgcaccaca tcaaactgtt tcagcgctgc
    44641 aatctgccga cgaataattt gcgcatcgta gacccacacc gggcagccaa attcagcggg
    44701 caaacgcagc agattttcgg cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat
    44761 aacaaactcc tgatgagtgc ttttttatga ttacgccaca tcataaaaag aataaaaaat
    44821 atcgatttat gtcgagtcta tgcaaaaatg atatggatta ccggattgcg agagagcgct
    44881 aatggccgcc gttaacttac gtcatattga aatttttcat gcggtaatga ccgccggaag
    44941 cctgactgag gcagcacacc tgctacacac ctcacagcca accgtcagcc gcgaactggc
    45001 gcgctttgag aaggtgatcg ggctaaaatt gtttgagcgc gtacgtggac gattacatcc
    45061 taccgtgcaa ggactgcgtc tgtttgaaga agtgcaacga tcctggtacg gactggatcg
    45121 cattgtcagc gccgcagaaa gtctgcgcga gtttcaccag ggagaactgt ctattgcctg
    45181 cctgccggtc ttttcgcaat cttttttacc gcagctcctg caaccctttc tggcacgtta
    45241 tcccgatgtc agcttaaata tcgtgcccca ggaatcaccg ctacttgaag agtggctctc
    45301 ggcccagcgt catgatttag gactcactga gacactccat acgcctgcgg gaacagaacg
    45361 taccgaatta ctctctttag atgaagtgtg tgtgttacct ccgggtcatc cgctggcgat
    45421 aaaaaaggta ttaacgccgg atgattttca gggtgagaac tacatcagcc tttcccgtac
    45481 tgacagctat cgccagttgc tggatcagct atttactgaa catcaggtta aacgacgcat
    45541 gatcgtagaa acccacagcg ccgcgtcagt gtgcgcaatg gtacgggcgg gggtaggtgt
    45601 ttcggtggtt aacccgctca ccgcactgga ttatgcggca agcggtttag tggtgcggcg
    45661 gttcagtatt gcggttccgt tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc
    45721 agcgctggtg caggcgttta gtgagcattt acaagcggga ttaccgaaac tggtcacttc
    45781 tcttgacgct attctgtcgt cagctacgac agcataaaag cgacagcatc ctcggcatgg
    45841 atcgccgcag tatcaaacac aggcagaaca ctgcgctctt ctggcaccag taaaccaatc
    45901 tctgtgcagc caaaaatgac accctgcgcc ccctgttctg ccaggcgagc aatcacttgc
    45961 gcataataag tgcgtgacgc ttcggtaaat tgccccagac acagttcttc aaaaataatc
    46021 tgattaattt tcgcccgttc atccgcttca ggaataagac aattgatgga aaattgttcc
    46081 gtcagccgcc cgcgataaaa atcctgttcc atggtgtaac gcgtacccag cagcgccaca
    46141 cgagtcattc cggccccggt aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc
    46201 agagagcaac gagactctat agcatccgcc actttgtgca tcgtattggt acataacaca
    46261 atgccttctg cgcccgcccg ctgtaagcca agcgccgcct cagccagaat atccccggtt
    46321 ttgtcccact caccgcgacg ctggcactct tctatttctt gaaaatcgac gctatgtagc
    46381 agcacttgtg cagagtgaag cccaccaagc cgctgtttaa tgccttcatt tatcagacga
    46441 tagtaaggaa tggtggattc ccagctcatt cctcccagca aaccaattgt tttcattcct
    46501 ctctcctgat gtgtgttacc gcaacaaggc tgaataagga acgagacaaa cgcctcaacg
    46561 gccaggtgcc caatctctat taacgaaaaa agggccggat gtacagcaca tccggcccgt
    46621 gaaatcagac gccgatattt ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt
    46681 ccagggtgac atttttggtt tccggaatga gccagaaagt gatgcccaca aacgcaatgt
    46741 tcagcgcagt gtagagccag aacgtaccgg cagcgccaat gctgtcgagc agcgtcagga
    46801 aggtcgcgcc gataatcata ttcgacaccc agttcgtggt ggtcgaacag gtaataccga
    46861 aatcgcggca tttcagcggc tgaatttcag agcacaggat ccacaccact ggcgcggcgc
    46921 tcatcgcata accggcaata cacatcatcg tcatgccaac agagagccag gacaagccac
    46981 tggaagccgt accgttatca aactgcatca ggcaatagcc cagcaccaga gtgcctaacg
    47041 ccatcacgct gaaaccaatt ttcagagccg gtttacgacc tgctttatct accgtaaaga
    47101 ccgcaatgaa ggtcgcgaac ataaaggtca gtcccacgac cagagtcgca atcatctgtt
    47161 gttctgtggt cgtaaagccc gccattttga agatacgcgg cgcgtagtac atgatgatgt
    47221 tcataccggt gaactgctgc atcgcctgca acaacatacc gagaaacaca gcacgacgga
    47281 cgttacggtt gatcttaaac agtgcccaac cgccctgttt taacttcagg ctttcacgaa
    47341 tttcgttgag ttcttctcgc gctttttccg acgtatcgcg cagcatacgc aacacttctt
    47401 ccgcctcaat atgacgcccc ttttccgcca gccagcgcgg gctatttggc aggaagacca
    47461 ccagaataat cagcaggact gctggtaaag caagaacccc caacattgcg cgccagttac
    47521 cgctataact gaacgctgta tcggataaaa acgccagcac gatgccgagt gtgaccatca
    47581 actggtacat actgatcatc ttacctcgaa cgttttcact tgccatttca gaaagataca
    47641 gaggagcggt gtaagacgcg atcccgacag caatgcccag caccacacga gcggcgatta
    47701 acatctctac gctggtcgca aaagcggacc ctatagaacc gagtacaaac aggatggccc
    47761 ccgccatcag gctgtattta cgccccaggc ggaacgacag ccaaccatta aacagcgcac
    47821 caattgctgc gccgagcatc atgctactaa ccacccattc ctgcaaacga ctggtcagca
    47881 caaagtgatc ggtaatgaac ggcaacgctc cggcgattac gccgatatca agaccaaata
    47941 acaatcctgc gaccgcagca gctaccgaaa caaacatatt catacgccgc gtatcccgca
    48001 aagaacgtgg cgttaaagca gattccgtat tgatagtaac cattttttcc tgccagcaga
    48061 gagtaagaca tagtgaaaaa atacgtgaac aactcacaca ggtgtcaggt cggaaacagc
    48121 ataaatatgg attaaattgc tgcgacatgt cgttatgtga tggatattcc aattttcaaa
    48181 ttaagttgaa ttattgagat tattattaac cacctaattt tacagcagat aaaattcata
    48241 aagttcatta attgataatt gatatggatt atttcataac catgatatgg attatgatga
    48301 tctacaggta taaaaaaccc tgccatgcgg cagggtcata aaagtaagaa gaatgaatta
    48361 acgcgccagc caaccgccat ccacggcaat ggtataacca ttcacataat ctgaagcgct
    48421 ggaggcaagg aacactaccg gccccatcag gtcactcggc agtccccaac ggccagctgg
    48481 aatgcggtcg agaatttccg cgctacgttg ttcatctgcc cgcaattgtt gagtattgtt
    48541 ggtcgccatg taacccggag ctatcgcatt aacattaatg ttgtgtttag cccattcgtt
    48601 cgccatcaat cgcgtcacgc ccatcacacc gctttttgat gcggtataag aaggcacacg
    48661 gatcccgccc tggaaggaga gcattgacgc gatattgata atcttgccgc cattgccttg
    48721 cgcgataaag tgtttcgccg ctgcctgaga catgaagaat acgctcttga tattcaggtt
    48781 catgacatcg tcccagtcct tttcgctgaa ctcgagagca tcttcgcggc gaatcaatcc
    48841 ggcgttattc accaggatat caatatgacc aaactcagct accgcgcgat ccagcagtgc
    48901 tggaatgcca tcaatctttc gcagatcggc ggtcaggctt aaaaaacgac gccccagcgc
    48961 cgtgacctgc ttgatggttt cagtcggttc aacgatgtta atgccaacaa tgtcacagcc
    49021 cgcttgcgcc agccccaacg ccatcccctg gcccagccca gtatcacaac cagtgacgac
    49081 cgcaacttta ccttcgagag aaaatgcact taaaatcata acaatacctt attcgtttat
    49141 gcccacaact agcgcaaatc tataacggcc acatggtcca tatcatcaaa gacctggttt
    49201 tcaccgacca tgccccagat aaaggtataa gctttggttc cgacgccgga atgaatcgac
    49261 cagctcgggg agatcaccgc ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc
    49321 tgccccatca tgtggaaaac gcaggcgtca tcatccatat tgaaatagaa ataaacttcc
    49381 atccggcgct cgtgagtgtg acacggcatg gtgttccaca agttacctgg agccagctcc
    49441 gtcagcccca tactcaattg gcaggtttcc agcacatccg ggacaaaata tttgttaatt
    49501 gtgcgacggt tactggtgag gttatcgcct aacgtgactg gagatacttc gtccggtgtg
    49561 acttttttgg tgggatacgt cgtatgcgcg ggtgcgcaat tgtaataaaa ctttgccgga
    49621 gtggcggtat caatactggc aaagacaact tcttttgcac ctttaccaac atacagggcg
    49681 tcgcggtgac cgatttcata gcattgacca tcgacagtaa tcgtaccggc accgccaata
    49741 ttgataacac ctaactcgcg acgttcaagg aaatagctta cgccgagttg tttaccaact
    49801 tccccgccaa cggaaaccgt tttagttacc ggcataatgc cgccaacaat aattcggtca
    49861 atgtggctgt aaaccatggt gtactcatcg gcgacaaata ccttttcaac caaaaattca
    49921 ttgcgcagcc cctgggtatc cagcgttttt gcgtgcgcac tgtggatgct ctgtcttacg
    49981 tccacatcaa cctccgataa tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg
    50041 tttcgttttc tctataaagg gatgatatgc ccatgggata gggttttcaa ttaaaaataa
    50101 aacaatgttt catttttaag ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt
    50161 catgagaatg gaaaaatgag ggagcccaga aaatgtatgt cgacttgcca gccatcatta
    50221 ttcaacagat taatgacggc agatatttat cggtaaggag gaaatgcaga gctactcgtc
    50281 acgttcaatg gtcaatgcca cgccctggcc cccgccgata caaagcgttg ccagcccttt
    50341 gcgggcatta cgtttcacca tttcatgaac cagagaaacc aggattcggc aaccggaagc
    50401 gcctatcggg tgaccgagtg cgatcgcgcc accattgaca ttgacccgac gctcatccca
    50461 ttcaagcatc ttgccaaccg aaagcgcctg tgcagcaaac gcttcattag cctcgataag
    50521 atcgacatca gccaactgcc agcccacacg ctccaggcaa cggcgggtcg catacaccgg
    50581 cgcaattccc atcaatgctg gatctacacc aacgctggca aatgcgcgga tgtgggccag
    50641 cacgggtaaa ttcaacgctc gtgctttggc ttcgctcatc atcattaccg cagctgcgcc
    50701 atcgtttatg gatgatgcat tacccgctgt cacagaaccg agactatcaa atgaaggatt
    50761 taaacgggct aagccttctg cactggcgtc agtgcgtggc tgttcatcgg tatcaataac
    50821 caacgtctga ccattacttt gggtcattac cgggacgatc tcatctttaa atcgcccggc
    50881 gtcaatcgcc gctcgcgctt tttgttgcga gctaagtgcg taagcatcct gcaactgacg
    50941 gctgatgcca tattcgcgag ccagattttc ggcggtgaca ccaatatgat aatcattgaa
    51001 ggcatcccac aacccatcgt gcaccagact gtcaaccaac tggctattgc caagctgtgc
    51061 accggtgcgg ctatcagtca gaacatgtgg tgcgcggctc atgttttcct ggccaccggc
    51121 gatgacaata tcagcctcgc cacactgtat cgcctgagta gccagatgca gtgccttaag
    51181 cccggaaccg caaacgtcat taatcgtgat tgcagaaacg ctattaggaa gaccaccttt
    51241 aatagccgat tgccttgccg gattctgccc tgcccctgca gtcaacacct ggccaagaat
    51301 cacttcatcc accgcatatg caggcacgcc ggtacgttct attaacgctt tcacgaccag
    51361 gctaccaagt tccacggcgg aatgacccgc taacgcacca cgaaagcagc cgataggtgt
    51421 ccgtaacgcc ccgacaatca caacgtcttt catcacaacc tcgcacaaat taacagtgca
    51481 atagtaaatg attgttaatt acagttatct taattgttta aaaaaagtga tttttatcac
    51541 aaaggaaata tgcctgagta gcagtcagag acataactgg cacgtaaggt ttgcaaccac
    51601 taacccacca atagagaggt agatagggcg ttaatctccc atacttaacc tggtttatgg
    51661 taaattgccc tccattttgt ttaatttgta gatgatacgt tcagataatg tctaatattt
    51721 ggtcaaaaga agaaactctg tggagtttcg cgctctacgg cacagccgtt ggtgcaggca
    51781 cgctcttcct tcctattcag ttaggttcgg caggggcagt ggtcctgttt attactgctc
    51841 tggtcgcctg gcctttaaca tattggccac ataaagcctt atgccagttc atcctctcat
    51901 cgaaaacatc agcaggtgaa gggataacgg gcgcagtaac acactactat ggcaagaaga
    51961 ttggtaatct gattaccacg ctgtacttca tcgccttttt tgtcgtcgtg ttgatatacg
    52021 cagtggcaat taccaactca cttacagaac agctggcaaa gcatatggtg attgatcttc
    52081 gcatccgtat gttggtgagt ctgggtgttg tattaatcct gaatctcatt tttctgatgg
    52141 gacgtcatgc cactattcgg gtaatgggat ttttggtatt cccattgatt gcctatttct
    52201 tatttctttc catttacctt gtcggtagtt ggcaacctga tctattaaca acccaggtag
    52261 agttcaatca gaataccctt caccagatat ggatatcgat tcccgtgatg gttttcgcct
    52321 ttagccatac gcccattatt tctacgtttg ccatagacag acgtgaaaaa tatggcgaac
    52381 acgctatgga taaatgcaaa aaaattatga aagtcgctta tctcatcatc tgcataagtg
    52441 tactgttctt tgtctttagc tgcctgcttt ctattccacc ttcgtatatt gaagcggcta
    52501 aagaagaagg ggttaccatt ttatcggcgc tttctatgct gccgaatgcc ccagcatggt
    52561 tgtcaatttc cgggattatt gtcgcagtag ttgccatgtc gaaatcattc ctgggtacgt
    52621 actttggcgt tattgaaggt gccacagagg tcgtcaaaac aacactacag caggttggta
    52681 taaagaaaag tcgtgcattt aaccgcgcac tatcaattat gttggtatcg ctgattacct
    52741 tcattgtttg ttgcattaac ccgaacgcga tttcgatgat ttatgcgatc agcggcccgc
    52801 tcattgccat gatacttttc atcatgccta cgctatcaac atatctcatc ccggcgctta
    52861 aaccctggcg ttccatcgga aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg
    52921 taatgttctt tagctaatat ccattgtgaa tggttcaaaa ttgctacaat tttgaaccat
    52981 tccatttttc ataatgttca tttgttatta ttccgcccta tcacttctct cgtcatatcc
    53041 tagaattatc agttagttaa acacacaaag agatctgaac atcttaaata agactattta
    53101 agatgcataa cttagattcg caagatatct tctggtcatt caaaaacaat ttgcgatata
    53161 gatattctca tactatgaga tagttatctg gaccactctc aaccatacaa gattaaatca
    53221 gtgaaacata atcatattta ataacgagaa atgcattttt aaatgtctta atcagaatta
    53281 tagcaaatac ag
//
LOCUS       DAZUCE010000007        51676 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZUCE010000007 DAZUCE010000000
VERSION     DAZUCE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.007"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..778)
                     /gene="chbR"
                     /locus_tag="ACHY03_000383"
     CDS             complement(<1..778)
                     /gene="chbR"
                     /locus_tag="ACHY03_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFS3903663.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKT"
     gene            complement(786..1136)
                     /gene="chbA"
                     /locus_tag="ACHY03_000384"
     CDS             complement(786..1136)
                     /gene="chbA"
                     /locus_tag="ACHY03_000384"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFS3903664.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1187..2545)
                     /gene="chbC"
                     /locus_tag="ACHY03_000385"
     CDS             complement(1187..2545)
                     /gene="chbC"
                     /locus_tag="ACHY03_000385"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFS3903665.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(2630..2950)
                     /gene="chbB"
                     /locus_tag="ACHY03_000386"
     CDS             complement(2630..2950)
                     /gene="chbB"
                     /locus_tag="ACHY03_000386"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFS3903666.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3249..3587)
                     /gene="osmE"
                     /locus_tag="ACHY03_000387"
     CDS             complement(3249..3587)
                     /gene="osmE"
                     /locus_tag="ACHY03_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFS3903667.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3789..4616
                     /gene="nadE"
                     /locus_tag="ACHY03_000388"
     CDS             3789..4616
                     /gene="nadE"
                     /locus_tag="ACHY03_000388"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFS3903668.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            4846..5733
                     /gene="cho"
                     /locus_tag="ACHY03_000389"
     CDS             4846..5733
                     /gene="cho"
                     /locus_tag="ACHY03_000389"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFS3903669.1"
                     /translation="MVRRLTSSRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5693..6268)
                     /gene="ves"
                     /locus_tag="ACHY03_000390"
     CDS             complement(5693..6268)
                     /gene="ves"
                     /locus_tag="ACHY03_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFS3903670.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6471..6956)
                     /gene="spy"
                     /locus_tag="ACHY03_000391"
     CDS             complement(6471..6956)
                     /gene="spy"
                     /locus_tag="ACHY03_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFS3903671.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7286..8254)
                     /gene="astE"
                     /locus_tag="ACHY03_000392"
     CDS             complement(7286..8254)
                     /gene="astE"
                     /locus_tag="ACHY03_000392"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFS3903672.1"
                     /translation="MDNFLALTLTSKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8247..9590)
                     /gene="astB"
                     /locus_tag="ACHY03_000393"
     CDS             complement(8247..9590)
                     /gene="astB"
                     /locus_tag="ACHY03_000393"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFS3903673.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNKASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVVRQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHFTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFA
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPTRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMVLVLPQECREHAGVWCY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNIGSVYPFQREGGGNG"
     gene            complement(9587..11065)
                     /gene="astD"
                     /locus_tag="ACHY03_000394"
     CDS             complement(9587..11065)
                     /gene="astD"
                     /locus_tag="ACHY03_000394"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFS3903674.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFAERQAVVERFAGLLERNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVYGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(11062..12096)
                     /gene="astA"
                     /locus_tag="ACHY03_000395"
     CDS             complement(11062..12096)
                     /gene="astA"
                     /locus_tag="ACHY03_000395"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFS3903675.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(12093..13313)
                     /gene="astC"
                     /locus_tag="ACHY03_000396"
     CDS             complement(12093..13313)
                     /gene="astC"
                     /locus_tag="ACHY03_000396"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFS3903676.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLQLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEDCASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAVACEHFVSRGSS"
     gene            13759..14565
                     /gene="xthA"
                     /locus_tag="ACHY03_000397"
     CDS             13759..14565
                     /gene="xthA"
                     /locus_tag="ACHY03_000397"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFS3903677.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDEEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14732..15442
                     /gene="ydjX"
                     /locus_tag="ACHY03_000398"
     CDS             14732..15442
                     /gene="ydjX"
                     /locus_tag="ACHY03_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFS3903678.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFKAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTAIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            15447..16124
                     /gene="ydjY"
                     /locus_tag="ACHY03_000399"
     CDS             15447..16124
                     /gene="ydjY"
                     /locus_tag="ACHY03_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFS3903679.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENTAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16139..16846
                     /gene="ydjZ"
                     /locus_tag="ACHY03_000400"
     CDS             16139..16846
                     /gene="ydjZ"
                     /locus_tag="ACHY03_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFS3903680.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            16846..17394
                     /gene="ynjA"
                     /locus_tag="ACHY03_000401"
     CDS             16846..17394
                     /gene="ynjA"
                     /locus_tag="ACHY03_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFS3903681.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17404..18570
                     /gene="ynjB"
                     /locus_tag="ACHY03_000402"
     CDS             17404..18570
                     /gene="ynjB"
                     /locus_tag="ACHY03_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS3903682.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFSTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQGLLEFTKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            18543..20078
                     /gene="ynjC"
                     /locus_tag="ACHY03_000403"
     CDS             18543..20078
                     /gene="ynjC"
                     /locus_tag="ACHY03_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFS3903683.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWMEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNMDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLTIFALTALVAKWVDYVRQ
                     GLR"
     gene            20078..20731
                     /gene="ynjD"
                     /locus_tag="ACHY03_000404"
     CDS             20078..20731
                     /gene="ynjD"
                     /locus_tag="ACHY03_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS3903684.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALPGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            20798..22105
                     /gene="ynjE"
                     /locus_tag="ACHY03_000405"
     CDS             20798..22105
                     /gene="ynjE"
                     /locus_tag="ACHY03_000405"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFS3903685.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSANWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(22114..22734)
                     /gene="ynjF"
                     /locus_tag="ACHY03_000406"
     CDS             complement(22114..22734)
                     /gene="ynjF"
                     /locus_tag="ACHY03_000406"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS3903686.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            22821..23228
                     /gene="nudG"
                     /locus_tag="ACHY03_000407"
     CDS             22821..23228
                     /gene="nudG"
                     /locus_tag="ACHY03_000407"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFS3903687.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(23194..23466)
                     /gene="ynjH"
                     /locus_tag="ACHY03_000408"
     CDS             complement(23194..23466)
                     /gene="ynjH"
                     /locus_tag="ACHY03_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFS3903688.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLNTNPLVWQRVKP"
     gene            23702..25045
                     /gene="gdhA"
                     /locus_tag="ACHY03_000409"
     CDS             23702..25045
                     /gene="gdhA"
                     /locus_tag="ACHY03_000409"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFS3903689.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFDLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(25162..26201)
                     /gene="ynjI"
                     /locus_tag="ACHY03_000410"
                     /pseudo
     CDS             complement(25162..26201)
                     /gene="ynjI"
                     /locus_tag="ACHY03_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(26329..28290)
                     /gene="topB"
                     /locus_tag="ACHY03_000411"
     CDS             complement(26329..28290)
                     /gene="topB"
                     /locus_tag="ACHY03_000411"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS3903690.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHVPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(28295..29338)
                     /gene="selD"
                     /locus_tag="ACHY03_000412"
     CDS             complement(28295..29338)
                     /gene="selD"
                     /locus_tag="ACHY03_000412"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFS3903691.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(29455..30006)
                     /gene="ydjA"
                     /locus_tag="ACHY03_000413"
     CDS             complement(29455..30006)
                     /gene="ydjA"
                     /locus_tag="ACHY03_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFS3903692.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            30167..32023
                     /gene="sppA"
                     /locus_tag="ACHY03_000414"
     CDS             30167..32023
                     /gene="sppA"
                     /locus_tag="ACHY03_000414"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS3903693.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            32177..33193
                     /gene="ansA"
                     /locus_tag="ACHY03_000415"
     CDS             32177..33193
                     /gene="ansA"
                     /locus_tag="ACHY03_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFS3903694.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            33204..33845
                     /gene="pncA"
                     /locus_tag="ACHY03_000416"
     CDS             33204..33845
                     /gene="pncA"
                     /locus_tag="ACHY03_000416"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFS3903695.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(33938..35296)
                     /gene="ydjE"
                     /locus_tag="ACHY03_000417"
     CDS             complement(33938..35296)
                     /gene="ydjE"
                     /locus_tag="ACHY03_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3903696.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPAMVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFMLGGIGILLAWLLSGKYFIESPRWLAGKGQIAGAECQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(35414..36172)
                     /gene="ydjF"
                     /locus_tag="ACHY03_000418"
     CDS             complement(35414..36172)
                     /gene="ydjF"
                     /locus_tag="ACHY03_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS3903697.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(36309..37289)
                     /gene="ydjG"
                     /locus_tag="ACHY03_000419"
     CDS             complement(36309..37289)
                     /gene="ydjG"
                     /locus_tag="ACHY03_000419"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFS3903698.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPQQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(37299..38246)
                     /gene="ydjH"
                     /locus_tag="ACHY03_000420"
     CDS             complement(37299..38246)
                     /gene="ydjH"
                     /locus_tag="ACHY03_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFS3903699.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(38251..39087)
                     /gene="ydjI"
                     /locus_tag="ACHY03_000421"
     CDS             complement(38251..39087)
                     /gene="ydjI"
                     /locus_tag="ACHY03_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFS3903700.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(39108..40151)
                     /gene="ydjJ"
                     /locus_tag="ACHY03_000422"
     CDS             complement(39108..40151)
                     /gene="ydjJ"
                     /locus_tag="ACHY03_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFS3903701.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(40168..41547)
                     /gene="ydjK"
                     /locus_tag="ACHY03_000423"
     CDS             complement(40168..41547)
                     /gene="ydjK"
                     /locus_tag="ACHY03_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3903702.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFIFLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(41574..42650)
                     /gene="ydjL"
                     /locus_tag="ACHY03_000424"
     CDS             complement(41574..42650)
                     /gene="ydjL"
                     /locus_tag="ACHY03_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFS3903703.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNAWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(43020..43292)
                     /gene="yeaC"
                     /locus_tag="ACHY03_000425"
     CDS             complement(43020..43292)
                     /gene="yeaC"
                     /locus_tag="ACHY03_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFS3903704.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(43334..43747)
                     /gene="msrB"
                     /locus_tag="ACHY03_000426"
     CDS             complement(43334..43747)
                     /gene="msrB"
                     /locus_tag="ACHY03_000426"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFS3903705.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            44089..45084
                     /gene="gapA"
                     /locus_tag="ACHY03_000427"
     CDS             44089..45084
                     /gene="gapA"
                     /locus_tag="ACHY03_000427"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS3903706.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            45168..46052
                     /gene="yeaD"
                     /locus_tag="ACHY03_000428"
     CDS             45168..46052
                     /gene="yeaD"
                     /locus_tag="ACHY03_000428"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFS3903707.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAERGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(46103..46957)
                     /gene="yeaE"
                     /locus_tag="ACHY03_000429"
     CDS             complement(46103..46957)
                     /gene="yeaE"
                     /locus_tag="ACHY03_000429"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFS3903708.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASKCKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(47047..47793)
                     /gene="mipA"
                     /locus_tag="ACHY03_000430"
     CDS             complement(47047..47793)
                     /gene="mipA"
                     /locus_tag="ACHY03_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFS3903709.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            48229..50163
                     /gene="yeaG"
                     /locus_tag="ACHY03_000431"
     CDS             48229..50163
                     /gene="yeaG"
                     /locus_tag="ACHY03_000431"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFS3903710.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            50276..51559
                     /gene="yeaH"
                     /locus_tag="ACHY03_000432"
     CDS             50276..51559
                     /gene="yeaH"
                     /locus_tag="ACHY03_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFS3903711.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT        12939 a        13457 c        12511 g        12769 t
ORIGIN      
        1 acgttttaat aaacaaactc gggctactat aaccggcctc gaacgcaata tccgtcactg
       61 aatagttggt catttccagt tgttttttgg caaaattaat acggatttca ttaataatct
      121 gcattggcgt tttgccataa tatcgttgag tcgcccgcgt caaatattcc tgtgatttgg
      181 ctgacaacgt caccatattc tccagcgccg attcactaaa ctgctcttta tcatgcatct
      241 tttctaccgt acttttcagc cactgcggta catcatcaat cacctgttct tcgcggtaat
      301 gacgtaaacg gttaatgaca taaaaagtaa ccatctcaac aaactcttcc agccctgttt
      361 cgcggaaatt caatgaagaa atcactgttt ccacataggt gagaaaagcg ttattggtcc
      421 ggtatacctg cgaagcaaca aagcaataag gcaacaacgg caggtaatgc tgctcaaaaa
      481 agcgtttact gatcccaacg ttcaatatac gcgtggcacc aaactcataa aaactttgat
      541 ggtgcgaacc taacggaata aaaacaaaat cgccccgttc cagtaacacg cgcttaccgt
      601 taatctcctg gaaataacgc ccggttaata ccagagtaaa ttcataatag tcgtgctgat
      661 gcagtccgct gatactctca gttttgttat agataaacac atggaagttt ttgccattaa
      721 acaactgctg ttctcgggca gtggcaattt ccggcgcgtt aatcactggc tgcatcatcg
      781 actccttatg ccttcagttt ttcatgaagc tcaattaatt cggtaatcag ttcacgcgca
      841 agcatggacg tcattaaatg atcctgagcg tggaccagca ccagactcac tttcatctta
      901 ccttcgcccg catcgccttc aatcagtttc gtctgtacca gatgcgcttc attcaatgcc
      961 atccgtgact gatccatcat ggcttttgct gcggcaaaat cgccctgctt cgcctgtttc
     1021 agtgccgcat acgccaggct gcgcgcttgt ccggagttga tgataagccc catcaccact
     1081 tcttccagct cttcagcttc cgtttgcgta tcgggaatat tatcgagatc catcatacat
     1141 cgttcctctc tattcttacc ggcacgatta cccgtaccgg catcgattaa aatttcaggg
     1201 cgttagcgat atcttcttcg ctctcttctt tatcaatcgc gttttgtgct ttgttagcca
     1261 ccacaacaaa gggcagataa attaacgttg cgataccaag gttgaagagt gcaaccagca
     1321 atgcggcgac gctaccgttg gtgttaaaga aggctcccag accggttggc atggtccacg
     1381 gtgcaatatt ggtcaccgga ggaataatgc ccatgtagta cgctgccagg gtgattgccg
     1441 ccagaatcgg ttgtaccagt acaaacggga taaacatcac cgggttcatg ataattggca
     1501 gaccaaacag aatcggttcg ttaatctgga agatgccgga cggcagcgcc agttttgcca
     1561 cctgacgata atcagcacgg cgagaggcta taaagatagc caggatcagg cctaaagtcg
     1621 caccactgcc cccaaggaaa ataaaggagt ccagcatcgg cttggcccag atatggaagg
     1681 tcttaccggc tgccagcgcc gcttcgacgg aaccatattg ctgataggtc gcgatatttt
     1741 ccagcgccca cggcgtcata atgccgttgt ccagtgcggt cagcgccagc gcgccatgaa
     1801 taccgaagaa ccagagcagt ggaacaaaga tcacataggc ccagcccacc acgctaccca
     1861 acgatgccag aggggttgag atggtatcca taatgatctg atggaagttg gtgccccagg
     1921 tattcaacgc ccaggcaata atccccatca cggaaagaat aataaagccg ggaattaatg
     1981 ccgagaagga acgcgatact gaagcaggta cgctgtcggg cagtttaatg acccaattgc
     2041 ggcggacaat aaaggtaaac atttctgcca ccaccaggcc aataataatc ccggagatga
     2101 tattcgcccc acctaaccag tttgcgccaa ccgcataggc ctcaccgaca ctatatgggg
     2161 tgacggtcat aaatgctgca acggataaca acccagccgc cagcgcatcg actttacgct
     2221 cttctgccag cgccatgcca ataaagaacg gtgccattaa agacattatc cctaatgttc
     2281 cgttatatac gttgccgcca atacctttca gaccattaag tgtttcaatg gttgaggcgt
     2341 cgaggcgaat acctaaggaa taaaaaaacg acccctcccc aaagcttaga aaaacgttgt
     2401 taattaatac aaacatggcc cccgcaaggg ttaacggcat taagcgaata aagccatttt
     2461 tgattgcatt aacgtgtggc tgctttccta ttttaactgc aaaagggagg agtacctttt
     2521 caagcgatgc aataacatta ctcatagaaa aataccctta aaaaccgcaa tttaaatatt
     2581 gcggtattga tttatgaaat aactctttga cgggaaaatt taaaataaat taatttgctg
     2641 cggctttttt aatcgctgca accgcagcct taagcacgcc taaaccatcg actttgccat
     2701 aaagcagcga gtcaattact tcaaccggtt tgttgggtaa caaacgctgg atttcgggca
     2761 acatataagc aatctgcggc cctaataaca cgacatcggc attctgacct ttttcaccag
     2821 ccagtgtttc cggaaatgct tcaataatga ccggaacttc atatttttct gcctgtgcgc
     2881 gcatttttga taccagtaaa gaggtagaca tgcccgcaga acaaaacaga taaatgtgtt
     2941 tcttttccat aaaactgccc tcatcgacga ttatctgtca gccagacact ccgcaagcct
     3001 taacctgctt ccatgctctg ggtaacttgc gaaaccaaca tgactttttt tgtggctgaa
     3061 aggagtatac ggtatagacc tgagcaatag tatttctttg accttataaa ttgtctctcc
     3121 ttgacctgcg gttatgaccc tcttcacatt tcgggcaaat aattcgcgaa gataattaag
     3181 aactcaggcc aaaaaaaacc ggcgcaatgg ccggtttccg ttgttactca agcgttcaga
     3241 cgaagtgatt acttcgcagc ctgtggatca gtgtcgtatt cagcacaggt ctgataaccg
     3301 gagttgatga catgtccggt atcatctaac gcgacaaagt aggtttctgc tttaccatca
     3361 cgttgaccca ggatgtaggt ctggcaagta ccacgagcat ggatcatgct cacttcagac
     3421 gaaggtttac ccgcaatttg tgcaacctgc gcccggctca tgcctttttt gacgtctttc
     3481 accacaggct gtacaaactg gtctttggta cgatcataag ccgtacaacc cgccagcatg
     3541 gttaataccg ccgctgcact cagaattcct gccatattct tgttcatatt ccgtcctctt
     3601 gtttatcagc gtgttagata agcctggaat acattgggcg ctttttcaag cccatgaacg
     3661 aaacggctcc gctttcagag gattcctgta tgacgtttta accaccattc agcccgctgt
     3721 cgcttgtcgt ttcagtggca acgggttagc tttaaggaag ttttgtcttt tctgtctgga
     3781 ggggttcaat gacattgcaa caacaaataa taaaggcgct gggcgcaaaa ccgcagatta
     3841 atgccgaaga ggaaattcgt cgtagtgtcg attttctgaa aagctacctg cgaacttatc
     3901 cgttcattaa atcactggtg ctcgggatca gcggcggtca ggactccacg cttgccggaa
     3961 agctgtgcca gatggcgatt aatgagctgc gccaggaaac cggcaacgaa tcactgcaat
     4021 ttattgccgt acgcctgccc tatggtgttc aggccgacga acaagattgc caggatgcca
     4081 ttgcctttat tcaaccggat cgcgtattaa ccgttaatat caagggcgcg gtattggcta
     4141 gcgagcaggc attgcgggaa gcaggcattg aactgagcga ttttgtccgt ggcaatgaaa
     4201 aagcgcgtga gcggatgaaa gcacaatata gcattgcggg tatgaccagc ggtgtcgtgg
     4261 tgggcaccga tcatgcagca gaagccatta ccggattctt cactaaatat ggtgacggcg
     4321 gtacggacat taatccgctg tatcgtctca acaaacgtca gggtaaacag ttactggcag
     4381 cattaggttg cccggaacac ctttataaga aagcgccaac ggccgatctg gaagatgatc
     4441 gcccttctct gccagatgaa gtggcactcg gcgtgaccta tgacaatatc gacgactatc
     4501 tggaagggaa aaacgtacct gaacaggtcg ccagaacaat agagaactgg tatctgaaaa
     4561 ccgaacataa acgccgtccg ccaattaccg ttttcgatga tttctggaaa aagtaataat
     4621 ttgcacatat tggattgtgc gaaaaagagt aatttgttca cgccggatgc ggcgtgaacg
     4681 ccttattcga cctacaaaac tatgcaaatt caatacattg caggagtcga ataggcctga
     4741 caggcgtagc acgtaagacg gtgtaacctt tgtcatcgac ccgcctcttt tttaatcgct
     4801 tcccgcctgt tacactggat agataaccag cattcggagt caacagtggt acggcgttta
     4861 acttcttcgc ggctcgaatt tgaagctgcg gcaatttatg aatatcccga acatttacgt
     4921 tcattcctta atgacttacc cacccgacca ggggtgtatc tgtttcatgg cgaaagtgac
     4981 accatgccgc tctatatcgg caaaagcgtt aatatccgca gtcgggtgct ttctcattta
     5041 cgcaccccgg atgaagccgc catgctacgg caatcccggc gaattagctg gatctgcacc
     5101 gcaggcgaaa tcggcgctct gctccttgaa gcgcgattaa tcaaagaaca acagccgctg
     5161 tttaataaac ggttgcgccg caatcgccag ctctgtgccc tgcaattaaa tgaaaagcgc
     5221 gtcgatgtgg tgtatgccaa agaggtggat ttttcacgag cccccaacct gtttggcctg
     5281 tttgccaata ggcgcgcagc tttgcaagca ttgcagagca tcgctgatga acaaaaactt
     5341 tgttatggcc tgttgggact ggaaccgtta agtcgcggtc gtgcatgttt tcgttcagcg
     5401 ctaaaacgtt gcgccggagc atgctgcggt aaagagagcc atgaggaaca tgcgctacgc
     5461 ttgcgccagt ctctggagcg tttgcgggtg gtgtgttggc cttggcaagg ggcggtggcg
     5521 ctgaaagaac agcacccgga aatgactcaa tatcatatta ttcaaaactg gctgtggctg
     5581 ggggcggtta attcgctgga agaagcgaca acgttaattc ggacacccgc cgggtttgat
     5641 cacgacggtt ataaaattct ttgtaagccg ctgctttccg gtaactatga aattactgaa
     5701 cttgatccgg cgaatgacca gcgagccagt tgatttcact gaacaacagt ttaccttgcg
     5761 gttgtaaaag ccgcagggtg tgcctgccat cgaaccagca cgcgccctgg tcggtagtga
     5821 gcagtttgtc gcccagttgc cacgccccgt taatcacaaa caccaccccg ccgcgtgagc
     5881 caaaggtagt aaacgtacgt tcggcaattc tgactttcgc cttacagaca tccaggcgcg
     5941 tcataatatt gaaatccatc gacatctgcc ctgccgtcag tttcgctttt accacctggt
     6001 ccgccgcaaa ggcaaaaggc tgtaacggtt ttaaggtatg gttaaagtgg tctgcgcttt
     6061 caaggaacat ctcgccgcct tccagcaacg tcactatcct ttccataccg ggaaacaaag
     6121 aaaactcccc attcgccgcg atggatgcaa tgctggcacg ccagtaaaaa tcacgttttg
     6181 ccggtgggaa agtgcaaatt tcgcgcgttt caccggcagc gtttcgccac agattcaccg
     6241 acattttacg catatcaaag tattccatac tcgctcccgg cccgttcttt tcattgttat
     6301 cgttcaggta accgtggatg gttatcggca actcgggtga tttacgttag tggtgatcag
     6361 gcttttttta caactgtttg agaagagaaa agaaaaccgc cgatcctgtc caccgcatta
     6421 ctgcaaggta gtggacaaga ccggcggtct taagtttttt ggctgaaaga ttattcagca
     6481 gttgcaggca ttttaccttt tgccgctgga cgttctgtca gacgcttctc aaaattagca
     6541 ttaaattgct ttttctgttc cggcgtcagg atgttgtaaa ttttgttctg ggtttccatg
     6601 tgcgccagca tgttagcttt gcgctgttct tccatttttg cgatctgcgc ttcagctttt
     6661 gctttatcga aggtatcgct ggcaatgatg tcatgcattg cgcggcgttc ttccagcggc
     6721 ggacgtttca tctggtcacg ctggcctttc atgatttcgc ggatctgctg tttctgcgcg
     6781 tcggtcaggt tcaggtcttt gaacatcatg tcctgatgcg gaccgaactt gcctttgtgg
     6841 tgcatcatcg gcttcgcgtc agccggtgct gcggtagtgg tgtctgcggc atgggccagg
     6901 ttagccgcgc caagagccag ggtagaggca acaaacagtg cagttaattt acgcatattc
     6961 tatatccttc ctttcagtta tttattacgg ctttcttaag tagcgtgccg tgttgacgag
     7021 attaacttta ctgactttag cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtgta
     7081 aaaaacactt ttgcgccaat tatggagaaa aaaagaaaat ttgatggaga gtgatgagcg
     7141 aatattacaa cacgatgatt ttgcagagat tatgaagaac tataccggat gactggtgat
     7201 aaataaagca aataaccagg attaatctgt attaatttat aagaaagcaa cttaataccc
     7261 gcagaatgat ttctgcgggt aagtattagc ttattttttc gagcattaat cccgcgcgta
     7321 atcccaacgc taccaacgga ttagggaata acacatactc tacatcatgg gttacggtaa
     7381 aacgttcctc accgtcctgc gccagcaatg ttcctttctc aaacggcata aaattcagcg
     7441 tgtcacttgc catatgcatt tcgaaggacg gcgagtggcg agtaatttgc gaaaccaccc
     7501 gataacggag cggcggtgtt ctcacgatac cgacactctc accagatagc agcgcagcaa
     7561 ttgcgctggc agttactgca aactggcgaa gatcgttttg cccaaagggc aacgctttgc
     7621 caagttccag cgtacaggcc agcgcgccaa aatgtctggc gctgaaatgg gtaaacgtac
     7681 caccaggctc ctgatggaac accagcgcct ccagccccgc cgcacccagc cacgtcagaa
     7741 atttctcgtc ccagggaatg tcgcgttgcg gtaatacacc gaactgcgga tgcaaggagc
     7801 cacgaattgc ggtatgtaga tcaaggtgcc agcgcacaga ttctttgccc tggtcataaa
     7861 aatcttccag gcactgttcc agttcgcgcg cccgacaggt ttccccgctt tcagcaaaca
     7921 gctgccaacg accgccaaac attcggttca tatcgctatg gcaataacgt ttcccttgct
     7981 tcagcgcagg aggattcccg aggatcacca gcaaccgcca ccgtaacggg atctcgccgt
     8041 gagatatcgc gccaagcaat gcgtccagca tctccacagg tgccgtctca ttaccgtgga
     8101 tccccgcaga aatcaccaga gcgccttgcg gtggcgttaa tggcgtcagt tccagcacac
     8161 catcgcccag ccagcgccag cgaacaccgt tgatttctcg ctcggtgatg acaggttttt
     8221 tactcgttaa ggtcagagca agaaaattat ccattgcccc ctccctcgcg ctggaacgga
     8281 taaaccgaac cgatattcag taattggctc aatacatcca gtgcttcccg cccttcgcgc
     8341 agcaattgcg ggtcggccag atcggcagca gtaagacgat cgcggtagta acgatccacc
     8401 cagtcattga gcgcattaaa caaggtatcg ttcatcatca ccgccggatt caccgcccgg
     8461 cgttcttctt ctgtcaatac cacccgcaac cgcaggcacg ccgggccacc gccattcgcc
     8521 atgctttcac gtaaatcaaa gacttttagt tcgctgatcg ggttgtcagc ggcaaggagt
     8581 tcattgagat aacaccatac tccggcgtgt tcccgacact cctgtggcag caccaacacc
     8641 atggaaccat catcgcggct cagcagttgg ctgttaaaca gataggtaga caccgcatcc
     8701 gacacggaaa cctgagttgc cggaacttct atcgccataa aaccgttcac ccgcgcacgc
     8761 aggtttgcca gtaactgtga ctggcgagcg aacgcctgtt ggtggcaaaa cagcacctgg
     8821 cggttactca cggcaatcac gtcattatga aaaacgccct ggtcgataac gtccgggttt
     8881 tgctgggcga aaatcacctg ttggggattc acctgattca gccttgccac cgcctcgctg
     8941 gcttcgcgag tctgccgcgc cggatagcgg gtaggtcggg tatcattgcc ctcttctcgc
     9001 ccgtagacaa aaagttgcat acccggttca ccgtaatgac cgccgagacg gttgtggttt
     9061 gccgccccct catcaccgag caaagccacc tgcggcaacg ccgaatggac agcgaatttc
     9121 tcttcatcgt taaaaatcgc ttttaacagc gattcggtga cgggcgcttc cagcgaacgg
     9181 tgaaatttat tgttcaggtt ggcgaccgtg aaatgcactt tgccatccag cgtatcggca
     9241 gatggcgcga tcgttgccgc attggctacc cacattggcg aagcggaact gacgctggaa
     9301 agccagtgcg gtgcctggcg tacaaccttt tccagtacct gctcatcgct accactgaat
     9361 cccaactgac gcagcaccgg aataaacgga cgctcgtgcg gcgggatcac ggcctggggg
     9421 aatcccgcat cggcaagggt tttcattttc agtaagccct gttttgccgc cagtcgcggg
     9481 ttagagacct gaaaacggtg acgggtagag gctttattac caaacgacag gcccgcgtaa
     9541 tgatgcgtca gccctaccag cccgtcgaaa ttaacttccc aggcgttcat cgcaccacct
     9601 catcggaaaa atccagcccg gggttaagtg tcgcgggcag cgttaacgaa tccgactcca
     9661 ggctcgccat cggccaggcg cagtaatctg cggcatacca ggcgctgggg cgatggttac
     9721 cggaagcgcc aatgccgccg aatggcgcgg tactggcagc gccggtaagc ggtttgttcc
     9781 agttaacaat ccccgcccgc gcctccagca acagttgatc gaatttttcc cgctcggggg
     9841 aaaccagacc gcaagagagg ccaaagcggg tgttgttcgc catcctgatc gcttcatcga
     9901 aattgtcata acgccagacg cgcaataacg gtccgaacac ctcttcatct ggtacgccag
     9961 caacgcctgt catttcaatg atccccggcg tcagcaacga tgtccctgct tgtaataagc
    10021 gcggcgcaag cagggttcgt ccgcccatcg cttccagttg ctgccaggca gtaaccacct
    10081 gctgtgcggc ctgttcagaa atcagcccgc caataaacgg ctgcggttcg tcatcccagt
    10141 tgcccggcgt taatcgctgg ctgacggcaa ccagacgagc aagaaacgca tcgccctgcg
    10201 ccccgctttt cagcaataaa cggcgggcac aggtgcagcg ttgaccggct gtgacaaacg
    10261 ccgactgaat ggtcagatgg acagcggcgt cgatatccgc cacctcatcg ataattagcg
    10321 ggttattacc gcccatctca agggcgagaa ttttctccgg ctgaccggag agctggcgat
    10381 gcagctggta gccggtattg gcgctgccgg taaacagcaa gccgtcgaga tcctccaacg
    10441 cactcagcgc ctgacccgtc tcgcgcccgc cctgcaccag gttcagcacg ccaggcggta
    10501 agccagcctg ctgccataaa cgcattaccg cttcgccact ccacggtgtc agttcgctgg
    10561 gtttaaagat aatggtgtta cctgccagca atgccggaac gatatgtccg ttcggcaaat
    10621 gaccagggaa attatacggc ccatacaccg ccagcacgcc gtgcgggcga tgtcgcaggc
    10681 tcgccgcgcc gtccggcatt tcgctacgct gctcgccggt acgaacgtga tacgccttaa
    10741 ttgaaatcgc gattttattg atcatcgccg tcacttcggt tgccgcttcc caacgcggct
    10801 tgccggtttc tcgggcaatg attgcggtta attcggcttt attcctttcc agcaatccgg
    10861 caaagcgttc gacaactgcc tgacgttcag caaatgagag ccgcgcccag cgcggaaacg
    10921 ccgcacgggc tgcgcgacaa gcctgcccga cctgagcggc atcggcatca ttgccttgcc
    10981 ataacacctc gcccgatacc ggattacgct tcacacgcga tgcaccctgg cccgttatcc
    11041 agtcaccgtt aatccataaa gtcatgctgt tttctcctct gcgcacaggc gcaccagacg
    11101 aacgcgatcc cctgcatggc atttgagggc atccagttgt gcggcggtta aaatcaaacg
    11161 ctcggttgcc ggatcggtac gcaccagcac cacgcggaaa tggtgataat tttcattggc
    11221 gaccaggcag gccggaaaat cgccctgcgc aggctgccct tctgccactt ccaccagccg
    11281 acttttacgg atggcgcgca cgcggtcgat gtcacactca agcgtcggcc caccgtcgaa
    11341 gatgtcgata tagttgcggt agcgaaaacc ttctttctcc agcaccgcgc gggcaggcgc
    11401 ggtttgcgga tgtacctgac cgatgacgtc ctgggcttcc tgggataaaa agtgcgtata
    11461 gatcggatgt ttcggcatca gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa
    11521 atcggcgcgg ctaaaatcca tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga
    11581 atagccgtgt tcgtcaatca ccccgcgcat ttcagcaacc actttgtcat taaacttgtc
    11641 gcgaaaagcc gccataaaca taaagcgcga tttcgacagc aaatagccgt tgccctcttt
    11701 gcgccagtcc gggtcgagaa acagcgtgca cagctcgctg ctgccggtgt gatcgttact
    11761 gagaaacaac gtcggcaatg cgttatagac attcagctct ttcgaagcgt gaaccagcgt
    11821 gccgacgcga tagttgtacc agggatcgtt cagcccaact gccacctcaa tggcacaaat
    11881 ccccgccacg gtgcctgtct cgctatcttc cagcacgaac acataaccct gctcactttt
    11941 aggcagttcg ccttgccagg ttttgattgc cctttcgata cgcgccgaaa gcgtggcttc
    12001 attggcggga agcgacgtca ggccgccgcc cgttttgctg gcaagctgca tcagcgccga
    12061 gacatctgag cgctcaacgg gacggatgac catcatgatg aacctcggct aacaaagtgt
    12121 tcgcaagcaa ctgcaaagcg atccagtccg gtcgtcacct cttcttcgct gacattgagc
    12181 gcaggcgcaa aacgcaccac gttgccaccc gcaatcagta ccatcacgcc tgctttcgcc
    12241 gcttcctgag agatctgttt cgcttgcccg gcgtaatcgg cattcagtac acagccaatc
    12301 agcaaaccta agccgcgaac ttcactgaac aaaccatagc ggtgattaat aatattaaga
    12361 cgctcaacaa accagtcgtg acgttgctta accccgttaa gcatctctgg cgtattgatg
    12421 agctccagca ctttgcctgc caccgccgag gccagcggat tgccgccata ggtggtgcca
    12481 tgagtgccaa cggtcatcac gctggcgcag tcttcggttg ctaacaacgc accgaccggg
    12541 aaaccgccgc ccagcgcttt ggcggtagtt aacagatcag gcgtcacgcc gtagtgcata
    12601 taggcataca gttccccggt gcgcccgacg ccggtttgta cttcatcaaa aatcaacaac
    12661 gcattgtggc gatcacacaa ttcacgcaga ccttgtaaaa atgcgttgct ggctggcacc
    12721 acaccgcctt ccccctggat gggttcgaca atcaccgcac aggtagaatc gtcaatcagc
    12781 gcgctggcag agttaatatc gttatatgca gcatgacgaa tatccggcgg cagtggcgca
    12841 aaatcctgtg aataggctgg ctgcccaccc gcactgacag taaacagcgt gcgaccatga
    12901 aacgcatttt taaacgccac gataccgctc ttatggctgc cgtagcggtc gtgagcgaat
    12961 ttacgcgcca gttttagcgc cgcttcattg gcttccgcac cggagttaca aaagaagacg
    13021 cgatcggcaa acgtggcgtc gatcaatttt ttcgccagtt gcagtaccgg ctcgttggtg
    13081 taaccgttgc cggtatgcca gaacttactc gcctgttcgt tcagcgcttc acgcagttcc
    13141 ggatgcgcat ggcccagcgc gttcaccgca atgccacccg cgaagtcgat atactctttc
    13201 ccctgctgat cccacaagcg cgaaccttcg ccacgtaccg gtataaaggg tgccggagcg
    13261 taaacaggta tcatccattc atcaaagttt tcacgcgtaa ttggctgaga catagcgacc
    13321 tctacagtaa ataattcgtt atttatatgt taataataag taatgtttgc gctgtaaatg
    13381 tagattgcag ggttcgtgcc agccagtgat aaaagtgcat aaacggcgga ggccaactgg
    13441 aaatcaagga gttataacca aaccatatgc atttaaagtg catataaagt gaatacgttt
    13501 gcgatgtggg tgaataaaaa gaataaaaaa cgcaatgtta tgcagaagta aaatataatt
    13561 ctggaattgt gatcattgac gaaatttact ggaaattact gcgccattct gacgcagcgc
    13621 gcaccaaaag cgggcatttt ttgcgccatc gttgacatca ttaacaacca tcgatcaaat
    13681 cacttaacaa caggcggtaa gcaaagcgaa attctgctac catccacgca ctctttatct
    13741 gaataaatgg cagcgactat gaaatttgtc tcttttaata tcaacggcct gcgcgccaga
    13801 ccccaccagc ttgaagccat cgtcgaaaag caccaaccgg atgtgattgg cctgcaggag
    13861 acaaaagttc atgacgatat gtttcccctc gaagaggtgg cgaagctcgg ctacaacgtg
    13921 ttttatcacg ggcagaaagg ccattatggc gtggcgctgc tgaccaaaga gacgccgatt
    13981 gccgtgcgtc gcggctttcc cggtgacgaa gaagaggcgc agcggcggat tattatggcg
    14041 gaaatcccct cgccgctggg caatgtcacc gtgatcaacg gttacttccc gcagggtgaa
    14101 agccgcgacc atccgataaa attcccggca aaggcgcagt tttatcagaa tctgcaaaac
    14161 tacctggaaa ccgaactcaa gcgtgaaaat ccggtgctga ttatgggcga tatgaatatc
    14221 agtcctggcg acctggatat tggcattggc gaagagaacc gtaagcgctg gctgcgtacc
    14281 ggaaaatgtt ctttccttcc ggaggaacgc gaatggatgg agagactgat gagctgggga
    14341 ttggttgata ccttccgcca tgcgaatccg caaacagcag atcgtttctc atggtttgat
    14401 taccgctcaa aaggttttga cgataaccgt ggtctgcgca tcgacctgct gctcgccagc
    14461 caaccgctgg cagaatgttg cgtagaaacc ggcatcgact atgaaatccg cagcatggaa
    14521 aaaccgtccg atcacgcccc cgtctgggcg accttccgcc gctaatttag cagctctcct
    14581 ggctcaaact gggtcaggag aattaacctt gagaaaaatc aacaaactgt cagtaatgat
    14641 ttgttgcctg ccgtcctttg ttataccgtc actgcgtttt tagttgtctg accacttctc
    14701 tattatcaag tttgatatag gaaactccac gatgaacgct gagcgtaaat ttctttttgc
    14761 ctgtcttatt tttgcgctgg tcatttacgc tatccacgct ttcggtttat tcgatctgct
    14821 caccgattta ccccacttac agacactcat tcgccagagc ggattgttcg gttatagcct
    14881 ctatattctg ttattcatca ttgccaccct ctttctgtta ccaggaagca tactggtgat
    14941 cgcgggagga atagtttttg gcccgctctt agggacacta ctctcattaa ttgccgctac
    15001 gctggcctcc tcgtgctcat tcctgctggc gcgctggatg ggacgtgatt tactgctgaa
    15061 atacgtgggc catagccata ccttcaaggc tatagaaaaa ggcattgcgc gtaacggcat
    15121 cgattttctt attctgaccc gcttaattcc gctgtttccg tacaatattc aaaattacgc
    15181 ttacggatta accgctattg ctttctggcc ctataccctt atttcggcat taaccaccct
    15241 gcccggaatc gtaatttata ccgtgatggc aagcgatctc gccaatgaag gcattacgct
    15301 gcgctttatt ttacaactct gtctggcggg cctggcgctg tttattctcg tccagctcgc
    15361 caaactctac gcccgacaca aacatgtgga tctgtctgct tcgcgccgca acccacttac
    15421 tcacccaaaa aatgaaggat agaacgatgt tgcaacatta ttcagtgtca tggaaaaaag
    15481 gactgactgc actctgctta ctggctgttg ctgggcttag cggctgcgat caaaaagaga
    15541 atacggcggc aaaagtggaa tacgacggac tttcgaacag ccaaccgctg cgcgtcgatg
    15601 ccaataacca tacggtaacc atgctggtgc aaattaatgg tcgtttcctc accgacgaca
    15661 ctcgtcacgg tattgtgttt aaagatggct ccaacggaca taaatcgctg tttatgggtt
    15721 atgcgacccc gaaagcattt tatgaagccc tgaaagaggc aggtggtacg ccgggcgaaa
    15781 acatgacgat ggataataaa gaaacgactc atgtcacggg cagcaaactg gatatttcgg
    15841 tcaactggca aggggcggca aaaacgtatt ccttcgatga agtgattgtt gacagtaatg
    15901 gcaagaaact ggacatgcgc tttggcggta atttaacggc agcagaagag aagaaaacgg
    15961 gttgcctggt gtgtctggat agctgcccgg tcggcatcgt cagcaatgca acatacactt
    16021 atggtgcggt tgaaaaacga ggtgaagtta aattcaaagg caatgcctcg gttctcccgg
    16081 cggataacac gctggcaacg gttaccttta aaatcaccga ataaagccag gataaaggat
    16141 gatgaagatg caatcgcgta aaatctggta ctaccgtata accctcatca tcctgttgtt
    16201 cgctatgctg ctggcatggg cgctgcttcc cggcgtccat gagtttatca atcgcagcgt
    16261 tgcggcgttt gccgccgtgg accaacaggg tatagaacgc tttattcagt cttacggcgc
    16321 actggcggca gttgtctcgt tcttgttgat gattttacag gccattgctg caccgctacc
    16381 tgcgtttttg atcacctttg ccaatgcgtc gctgtttggc gcgttctggg gcggcttgct
    16441 gtcgtggacc agttcgatgg ccggcgcggc actgtgcttt tttatcgcca gagtgatggg
    16501 ccgcgaagtg gtggaaaaat taaccggcaa aaccgtactt gacagtatgg acggcttttt
    16561 cactcgctac ggcaaacaca ccatcctggt ctgtcggtta ttgccttttg tccctttcga
    16621 tccaatcagc tatgctgccg gtttgacttc aatacgtttt cgctcgtttt ttatcgccac
    16681 cgggcttggt cagttaccgg cgactattgt ttattcctgg gcgggcagca tgttaacagg
    16741 cggtaccttc tggtttgtca ccggactgtt tattctgttt gccctgaccg tggtgatttt
    16801 tatggcgaag aaaatatggc ttgagcgcca gaagaggaat gcctgatggg tttaccgccg
    16861 cttagcaaaa ttcctttcat tttacgtccg caggcgtggc tgcatcgtcg ccattacggc
    16921 gaggtgctaa gccccattcg ctggtggggg cggatcccgt ttatctttta tctggtgtcg
    16981 atgtttgttg gctggctgga gcgcaaacgc tcaccgctcg atccagtggt acgatcgctt
    17041 gtcagcgcgc gcattgcgca aatgtgcctg tgtgagtttt gtgtggatat caccagtatg
    17101 aaagtcgccg agcgcaccgg cagcagcgat aaactgctgg cagtggctga ctggcggcaa
    17161 agcccgctct ttagcgatga agaacggctg gcgctggagt acgccgaagc cgcaagcgta
    17221 acgccgccaa cggtcgatga tgccctgcgt acccgactgg ctgcgcattt tgacgctcag
    17281 gcgctcaccg aactgacggc attgatcggc ctgcaaaacc tgtcggctcg ttttaattct
    17341 gccatggaca ttcccgctca gggactgtgc cgtattccgg aaaaacgttc ttaaggagag
    17401 atgatgcgcc attgtgggtg gttgctggga ttgttatcgc tgttttctct ggcaacacat
    17461 gccagtgact ggcaagaaat taaaaatgag gccaaagggc aaactgtctg gtttaacgcc
    17521 tggggcggcg ataccgcaat taaccgctat ctcgactggg tgagcggcga gatgaaaacc
    17581 cattacgcta taaacctgaa gattgtccgt ctggcggatg ccgcagacgc ggtgaagcgc
    17641 attcagaccg aagccgcagc cggacgtaaa acgggcggtt cggtggatct gctctgggtg
    17701 aacggcgaaa acttcagcac cttaaaagag gccaatttac tgcaaaccga ctgggcagag
    17761 actctgccca actggcgcta tgtcgacaca cagctgccgg tgcgggaaga tttttcagtg
    17821 cctacagaag gggctgaatc gccctggggg ggcgcacaac tgacatttat cgcccgccgc
    17881 gatgttacgc cacagccacc acaaacgccg caaggcttac tggagtttac taaagccaat
    17941 cccggcacgg ttacctatcc gcgcccaccg gactttaccg gcacggcgtt tcttgaacag
    18001 ttgctgatta tgctgacgcc cgatcccgcc gcattaaaag aagcgccgga cgatgcgact
    18061 ttcgcccgtg tcactgctcc cttgtggcaa tatcttgatg cgctgcatcc gtatttgtgg
    18121 cgcgaaggaa aagatttccc gccttcgccc gcgcggatgg atgctctgct gaaagccggc
    18181 acattgcgcc tgtcgctgac ctttaacccc gcgcatgcgc agcaaaaaat cgccagcggc
    18241 gatttgcctg caagcagtta cagttttggc tttcgcgagg ggatgattgg caacgtgcat
    18301 ttcgtcacca ttcctgccaa cgcgaatgcc agtgctgcgg cgaaggtagt tgccaatttc
    18361 ttgctctcac ccgatgcgca attgcgtaaa gcagatcccg ctgtctgggg cgatccttct
    18421 gttctcgatc cgcaaaaact gcctgatggg cagcgcgaaa cattgcaatc aagaatgccg
    18481 caagatctgc cgccggtact ggctgaaccg cacgcaggtt gggtaaatgc gctggaacaa
    18541 gaatggctac accgttacgg tacgcattaa tctttttgct gtgggcgatg gtggcggtga
    18601 tttatgcacc gctgatcccg gcagctctca cgctgatatc gcctgctttg tcgttgacac
    18661 actggcaggc gttatttgcc gatccacagt taccgcaggc attactggca acgctggtgt
    18721 cgacaaccat cgcggcggtc ggggcgttgt tgattgccct gctggtgatt gtggcgctgt
    18781 ggcctgggcc gaaatggcag cgtatgtgca cccgtctgcc gtggctgctc gccattcccc
    18841 atgtggcttt tgccaccagc gcccttctgc tctttgctga cggagggctg ctttatgact
    18901 atttcccgta tttcactccg ccaatggaca aattgggtat tgggctgggc ttcacgctgg
    18961 cggtgaaaga aagcgcattt ctgctgtgga tcttagcagc ggtattgagt gaaaaacggc
    19021 ttttacagca agtcatcgtg ctggattcgc tgggctacag ccgctggcaa tgcctgaact
    19081 ggctgctgtt gccctccgtc gcgcctgcgc tggcaatggc gatgctggcg attgttgcct
    19141 ggtcgctgtc ggtcgtagat gtggcgatta ttcttgggcc aggtaatcca ccgacgctgg
    19201 cagtaattag ctggcagtgg ttaactcagg gcgacgccga tcaacaaacg aaaggcgcac
    19261 tcgccagcct gctgctgatg ttgttactcg ccgcctacgt tttgctgggc tatctgctat
    19321 ggtgtacctg gcggcgtact attccccgcg tagatggcgt tcgtaagcct gccacgcctt
    19381 tattgccagg cattaccctg gcgagttttt tacccttgac cggtgtgctg tgtgtggttc
    19441 tgctggcgat cctcgcggat cagtcgacga tcaatagtga agcgctcatc aacagcctga
    19501 caatggggct ggtggcgaca ttcatcgctt tgctcctgtt actgctgtgg atggaatggg
    19561 ggccacagcg tcgccagttg tggctatggt tgcccatttt attaccggca ctgccgctgg
    19621 tggcgggcca gtacacgctg gcgctatggc tgaacatgga tggaagctgg acggcggtgg
    19681 tctgggggca tctgctgtgg gtaatgccgt ggatgctgtt tatcctgcaa cccgcctggc
    19741 agcgcattga ttcacggtta attttgattg cacaaacact gggctggtcg cgggccaaaa
    19801 tcttctttta cgtgaaatgt ccgcttatgc tacgccctac gctgattgcc ttcgcggtgg
    19861 gattttcagt cagtattgcg cagtatatgc cgacgctgtg gctgggcgcg gggcgttttc
    19921 cgacgctcac cactgaggcg gtggcattaa gcagcggcgg cagcaacggt attctcgccg
    19981 cccaggcttt atggcaactg ctattaccgc ttactatttt tgccctgacc gcgttagtcg
    20041 caaaatgggt agattatgtc agacaaggac tccgctaatg ctctgcgtga aaaatgtttc
    20101 gctacgttta ccagaaagtt gcttgctgac aaacgttaac tttacggtgg ataaaggtga
    20161 cattgtcacg ttaatggggc cgtctggctg tggaaaatcc actctgtttt catggatgat
    20221 tggtgcactg cccggacagt tttcttgtac aggtgagcta tggctcaatg agcaacggat
    20281 tgacatgcta cccaccgcac agcgtcagat tggcattctt tttcaggatg cactgttatt
    20341 tgaccagttc agtgtcgggc aaaatttact gctggcgcta ccggcgacac ttaaggggaa
    20401 tgcccgacgt aatgccgtga atgatgcact tgagcgttca ggccttgacg gagctttcca
    20461 tcaggatcct gccactttgt ctggcggtca gcgagcgcgc gttgctctgc ttcgcgccct
    20521 tctcgctcag ccaaaagcgt tgctactgga tgagccattc agccgtcttg atgtggctct
    20581 acgtgataat tttcgccagt gggtgttcag cgaagttcgc gaactggcga tccccgtcgt
    20641 tcaggtaacg cacgatctcc aggatgttcc tgctgatagt tctgttctgg atatggcgca
    20701 gtggtcagaa aattacaaca aactgcgata acgcaaagtt tttcccaatg cgtcagttca
    20761 gaatggcgca ctcaaaacta caatgtcggg attttcgatg aaacgtgttt ctcaaatgac
    20821 cgcgctggca atggctttag ggctggcttg cgcttcttcg tgggccgctg aactggcgaa
    20881 gcctcttaca cttgaccagc ttcaacaaca aaatggcaaa gcgatagata ctcgccccag
    20941 cgcgttttat aacggctggc cacaaacctt aaatggccct tctggtcatg aacctgccgc
    21001 cttaaacctc tctgctaact ggctcgacaa aatgagcacc gaacagctca acgcgtggat
    21061 caagcaacat aacctgaaag ccgatgcccc ggtggcgctg tacggtaatg gcaaagatgt
    21121 cgacgccgtc aaaacgcgac tgcaaaaagc aggttttacg catatctcca ttctgagtga
    21181 cgcgctaagc gaaccttccc gtctacaaaa actgccgcac tttgagcagc tggtttatcc
    21241 gcaatggctg cacgacctgc aacaaggtaa agaggttacg gcgaaacctg ccggtgactg
    21301 gaaagtcatt gaagcggcct ggggcgctcc taagctttac cttatcagcc atattcccgg
    21361 cgctgactac atcgatacca acgaagtgga aagtgaaccg ctgtggaaca aagtttctga
    21421 tgaacaacta aaagcgatgc tggcaaaaca cggcattcgc catgacacca cggtcattct
    21481 gtacgggcgt gacgtatacg ctgcagcgcg tgtggcgcag attatgcttt atgccggcgt
    21541 gaaagatgtg cgcctgctgg atggcggctg gcaaacctgg tccgacgcgg gactgcctgt
    21601 tgagcgagga acgccaccga aagtgaaagc ggaaccggat ttcggcgtga agatcccggc
    21661 acaaccgcag ttgatgctcg atatggaaca ggcccgtgga ctgctgcatc gccaggatgc
    21721 atcgctggtg agcattcgtt cgtggccgga gtttatcggt acgaccagcg gttacagcta
    21781 tattaaacca aaaggtgaaa tagccggagc acgttgggga cacgctggta gcgactcgac
    21841 gcatatggaa gatttccata acccggatgg caccatgcgt agcgccgatg atattaccgc
    21901 tatgtggaaa gcatggaata tcaaaccaga tcagcaagtt tcattctact gcggcaccgg
    21961 ctggcgcgcg tccgaaacct ttatgtacgc acgagcaatg ggctggaata acgtctccgt
    22021 ttacgacggc ggctggtacg aatggagcag cgatccaaaa aatccggtag caaccggtga
    22081 acgcggcccg gacagcagca aataacattg caattactga cgctggagcg acttcagcgt
    22141 cagataccca ctccacaccc gcgtaaaggt cgtcatccag cacaacgcac cgaagatcca
    22201 cgcaaaccac gcaaaccatg cagggaataa acatcccagc acaaacagta agatcgtttc
    22261 tgtgccttcg gttaatcccc ccagataata aaacgattta tgcgcataac cgggattatc
    22321 aatctgatgt ttcgccgcca gcgcagcaaa agcgagaaaa ctgctacccg tgccgataaa
    22381 cgcaaacagc agccagccgc ctgccagggc gttttgctgc ggtgcggcga ggataaaccc
    22441 aaaaggcacc agcgcgtaaa aaagaaaatc gagagaaata tcgagaaagc cgcccgcgtc
    22501 ggtaagccct ctgcgccgcg ccagtgcgcc atccagacca tcaagcagac gattcaacaa
    22561 aatgacgact agcgccgcca gataccagcc cagagccaga aacggcagag ccagcacgcc
    22621 gatggcaaat cccactaatg ttaaaccatc tggcgtaatg cccggtttat caagaacccg
    22681 cacgcactga tgcaacaacg gtttaatccg gggatgaaga tggcggtcta gcacaggcac
    22741 tccttaaata taaagccttt ctgattgagc aacagtgcgg atattatggc atttttcgct
    22801 tatctgcccg tgtgtaattt atgaaaatga ttgaagttgt tgccgccatc attgaacgtg
    22861 atggcaaaat tttactcgcg caacgccccg cccagagcga tcaggcggga ttatgggagt
    22921 ttgccggtgg taaagtcgag ctggatgaaa gccagcagca ggcgctggtg cgtgagttaa
    22981 acgaagaatt gggcatcgaa gcaactgtgg gtgagtatgt tgccagccat cagcgagaag
    23041 tttcggggcg gattatccat cttcatgcct ggcacgtacc cgacttccac gggacgttac
    23101 aggcacatga acatcaggcg ctggtctggt gctcacctga agaggcgctg caatatccgc
    23161 tggcccctgc tgacattcca ctattagagg catttatggc tttacgcgct gccagaccag
    23221 cggattagtg ttaagggttt tgtcatcacg ctggcattgc agcagtattc cctcggcttt
    23281 aattaccgct ccttcagaat aatttttatc ctgataaaca cagcactgag tacagggctg
    23341 cgctgactgc ccgcctgaac tgaacacttc tggcggtacg tttacctcca cgtccggacg
    23401 ataatgcggg ttagccagtg caattaatgg aaatgctaat actacggcga acaatgctcg
    23461 actcacaggg aactccttaa cgttattgtc tctgctactg ataacggtag ccgggtggca
    23521 aaactttagc gtctgagtta tcgcatttgg ttatgagatt actctcgtta ttaatttgct
    23581 ttcctgggtc atttttttct tgcttaccgt cacattcttg atggtatagt cgaaaactgc
    23641 aaaagcacat gacataaaca acataagcac aatcgtatta atatataagg gttttatatc
    23701 tatggatcag acatattctc tggagtcatt cctcaaccat gtccaaaagc gcgacccgaa
    23761 tcaaaccgag ttcgcgcaag ccgttcgtga agtaatgacc acactctggc cttttcttga
    23821 acaaaatcca aaatatcgcc agatgtcatt actggagcgt ctggttgaac cggagcgcgt
    23881 gatccagttt cgcgtggtat gggttgatga tcgcaaccag gtacaggtca accgtgcatg
    23941 gcgtgtgcag ttcagctctg ccatcggccc gtacaaaggc ggtatgcgct tccatccgtc
    24001 agttaacctc tccattctca aattcctcgg cttcgaacaa accttcaaaa atgccctgac
    24061 tactctgccg atgggcggag gtaaaggcgg cagcgatttc gacccgaaag ggaaaagcga
    24121 aggcgaagtg atgcgtttct gccaggcgct gatgactgaa ctgtatcgcc acctgggcgc
    24181 ggataccgac gttccggcag gtgatatcgg ggttggtggt cgtgaagtcg gctttatggc
    24241 ggggatgatg aaaaagctct ccaacaatac cgcctgcgtc ttcaccggta agggcctttc
    24301 atttggcggc agtcttattc gcccggaagc taccggctac ggtctggttt atttcaccga
    24361 agcaatgcta aaacgccacg gtatgggttt tgaagggatg cgcgtttccg tttctggctc
    24421 cggcaacgtc gcccagtacg ctatcgaaaa agcgatggaa tttggtgctc gtgtgatcac
    24481 tgcgtcagac tccagcggca ctgtagttga tgaaagcgga ttcacgaaag agaaactggc
    24541 gcgtcttatc gaaatcaaag ccagccgcga tggtcgagtg gcagattacg ccaaagaatt
    24601 tgatctggtc tatctcgaag gccaacagcc gtggtctgta ccggttgata tcgccctgcc
    24661 ttgcgccacc cagaacgaac tggatgttga cgccgcgcat cagcttatcg ccaatggcgt
    24721 taaagccgtc gccgaagggg caaatatgcc gaccaccatc gaagcgactg aactgttcca
    24781 gcaggcaggc gtactgtttg caccaggtaa agcggctaat gctggcggtg tagcaacgtc
    24841 aggcctggaa atggcacaaa acgctgcgcg cctgggctgg aaagccgaga aagttgacgc
    24901 acgtttgcat cacatcatgc tggatatcca ccatgcctgt gttgagcatg gtggtgaagg
    24961 tgagcaaaca aactacgtgc agggcgcgaa cattgccggt tttgtgaagg ttgccgatgc
    25021 gatgctggcg cagggtgtga tttaagttgt aaacgcctga tggcgctacg cttatcaggc
    25081 ctacaaatgg gcataattca ttgcagttac gctctaatgt aggccgggca agcgcagcgc
    25141 ccccggcaat atttcaggct tttatgagta tttaacggat gatgctcccc agggaacatt
    25201 tcttatgggc caacggcatt tcttactgta gtgctcccaa aactgcttgt cgtaacgata
    25261 acacgcttca agttcagcat ccgttaactt tctgcgatag cagcagatat gccagtaaag
    25321 aaatcccatt agactatttt tttgataatc ttcttcgctt tcgaacaact cgtgcgcctt
    25381 tcgagaagca agcattatat aatgccaggc cagttcttct tcaattgtcc cgttttgaaa
    25441 agctgtgctt gatatcgaga tcatccatga taattccgcc gcccatatta gcttcgccga
    25501 ggatttaccg gagctatgat tagcgcaatc agagatatag tctgagggaa aaacagcaaa
    25561 tttattcaac aaggcgataa cctgctctgg ggcttcctcc atgtttgctt taaaggtatt
    25621 ggctccatgg tcgccagaaa gaaaatgctc cattaaggca caataacttt cgctatcttc
    25681 gataccccat tgatcctcta aagactcgcg tcttttactt atgatatcga tcgagtcaaa
    25741 aggaagcaca tgatattgga aggtatcttt gccaggttca ggctttcgcg gccagaactc
    25801 cagcgtttca gaccattgct tatgatagaa tcgataaggt gcgatcaatt gtagcgcctg
    25861 taacttctcg atactgagcg gctcaatacc tttagcctga taataatgca gttgttcttt
    25921 ttttgcttta aaaccggccc gaacaataag ccccatcgta attaatagat aaagaaaaga
    25981 gcatcccgca gtaatcaggc ctctttcatt caaaccgttg gatgttatcg ctgcgaatac
    26041 aaacattaca gcgacaacac atgttaaata accccccact tacaaagcag catggcctta
    26101 ttttctttaa tcatccgttc aaaattacta ttaaatattt cccagccatt aaaagaatac
    26161 ttctcgctcc caggatggtt ttgtaataaa acttttttca tcacattccc tgttattaca
    26221 tactttagag gcgataattt tatccatgca aaaaaaatat ccaacgaatt ttcttgatcc
    26281 gctggatatt tcagaatatg actcgaatag cacgaaagat tcgctcgctt acgctatcgc
    26341 cccgcttccg gcttcatctg ctggcggact ttttttcgca ctacgtttac gcggtgcagc
    26401 ctttttctta tcagcactgc caccactgcc cggagccaca atgccgcgaa actgccgcac
    26461 cggagtacgt ttggcttgat caataagctg atatagcgtc cccaccagcg gctgcataaa
    26521 gtcctgatag cgacactgct tttcgctgat ttgcgtcagc accgattccc agtgcgcggt
    26581 catgtccggt cgcgtagcca tttctggcag cgaatggaat agcgcttttc cggcgtcggt
    26641 ggagtggata tagcgccctt ttttggtgag gaaaccacgc ttgaacaaca gttcaataat
    26701 cccggcacgc gttgcttctg tccccagacc atcggtcgca cgaaggatct ttttcagatc
    26761 tttgtcctgc acaaagcgcg cgatcccggt catcgccgaa agcagtgttg catcggtaaa
    26821 atggcgaggc ggctgggttt gccgctctac cacttcacct ttttcacaca gcaactcatc
    26881 gcctttcgcc accacaggca atggcgtacc gtcgttttct tcatcgcgct ctttgctgcc
    26941 taacagcgtg cgccagcctg cttcagcaag aaaacgggct ttagcgacaa atttgccttt
    27001 ggcaatgtcc agttcgataa cacacttgcg gaacaccgca tccgggcaga actgcatcag
    27061 atactgacgg gcaatcaggt tatagacctt cgcttcgttc tccgtcaggt tgatcgcaga
    27121 actccgtgcg gtcggaatga tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg
    27181 gttgcgtata tctggatcta ccactggctg cggcaacaga tccggtacat gaacgctgat
    27241 ggcattcatc accgcgtggc gtccggcaaa atgttcttct ggcaaatagc gacaatcgga
    27301 acgcggataa gtgattagct tgtgcgtttc gtacagcttc tggcagatat caagcacgtt
    27361 ctgcgcgctc agaccaaagc gttttgccgc ttcaatctgc aacgccgaaa gcgaaaacgg
    27421 cagcggcgcg gattctgatt cccgtttatc gttatagctg gtgactatag ccggttgacc
    27481 actaatgcgg ttaaccacat gctccgccag tggacgatgt aacaagcgcc cttcttcatc
    27541 ctggtacggt tcacacgctt cgctcggttg ccagatagcg gtaaaccgct catcggcagg
    27601 tgtcacgata tgcgctttga cttcaaagaa gtctttcgcc acgaagtttt caatctcttc
    27661 atcgcggcgc accaccagcc cgagcacggg tgtctgcacg cgtcccacgg aaagtacgcc
    27721 ctgataaccg gcattgcgac cgagaatggt atacgcacgg gtcatgttga tgccgtatag
    27781 ccagtcggca cgcgctcgcg ccagcgcaga aacgcacaat ggcacaaact cactgttgga
    27841 acgaagacgg tcgatcgccc gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg
    27901 ctgcacctgc tggcgctttt ccggtgccag ttgtagatag tccagcactt catccaccag
    27961 cagttgccct tcgcgatccg ggtccccggc gtgaacgatt tcgctggctt catgcaggaa
    28021 ccgtttgatg acgttaagtt gtttggtcac ggagggtcgg ggctgtaatt gccacttttc
    28081 cgggacaatc ggcaaatccg caagattcca gcgcgcatag cggctgtcgt aggcgtctgg
    28141 ctgcgcctgc tcaagcaggt gaccgataca ccaggtcacc acctgaccat taccgcactc
    28201 gataaagcca tcgcctttac ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag
    28261 actcggtttt tcggcaataa acaaccgcat tgaattaacg gatctcaacc atggcacgac
    28321 cgccgcgcgc tggcaccagt tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg
    28381 tagctttaac ctcattttct gcttccggca tgaccgccag cagcaaaccg ccagaagttt
    28441 gcggatcgca cagcaaatcg cgcacttcac gcggcatttc acccatcaga tgaccgtagc
    28501 tggcaaagtt acgttcggtg ccgccaggca ctgcgcccaa cttaatgtac tcttcaaccc
    28561 ccggcaattt tgggatcgcg tcatagtcaa cgcgcgcctg cacacctgcg ccctgacaca
    28621 tttcgctcaa atgacccagc agaccaaaac cagtaacgtc ggtcatcgct tttacgcctt
    28681 cgatgttggc aaaggacgcg cctgcgatgt tcatccggca catcacttcc gtcgccagtc
    28741 cctgatgttc tggtttcaac agtgattttt tctcagccgt ggtaagaacg ccgatcccca
    28801 acggtttcgt caggaacagt ttgcatccgg cttgtgcggt actgtttttc ttcacccgct
    28861 cggtcggtac gatccccgtt accgccagac caaaaatcgg ctccggcgca tcgatggagt
    28921 gaccgccagc cagcgcaata cccgcctgac ggcatgcata gcgtccacct tcggttactt
    28981 cgcgggcaat ttccggggaa agtttgttaa tcggccagcc gaggatcgca atcgccataa
    29041 tcggtttgcc ccccatcgcg aagatatcgc tgatggcgtt agtcgccgca atgcggccaa
    29101 aatcgaaagg attatcaacg atcggcataa agaagtcggt ggtactgata acgctggtgc
    29161 cattgcccag atcgtacacc gccgcatcgt cgcgggtttc attacccaca agcaaattcg
    29221 gatcaacaaa cttcgcctgc tcactgtgca ggatggtttc caacactttt ggggaaattt
    29281 tacagccgca accagctccg tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg
    29341 acatctcctg tcaatgcaat ccgggtatgg taaccctcat tccgtgaagt gataagtgag
    29401 agtgtctgaa ttcctgcgcc tttgctcaca atccagacag tttcgcgaca attatcagaa
    29461 ataagttaca aacggcgtcg ggtccgggac gttaatcgac gtagatgctt tcagctgcgg
    29521 cgtaccgagg tagagaaaac cgacaatttt atcctgctca cggcaaccga atgcttcacg
    29581 cactaccgga ctttcagtta atgcgccact gcgccagatg ccgccaaacc cctgggcaac
    29641 ggctgccatt tgcatcgcca tgaccgcgca tccggcagac atttcctgtt cccagcgcgg
    29701 gactttatga ttttcttcgc atttcgccac caccgtgatg atgagcggtg cgcggaacgg
    29761 cgcattacgg gctttgtcga tagctttgtc atcactaccg gcagcaatcg ccccctgttc
    29821 cagtacggcg ctgaaacgct cgcgcccttc cccttcaatc acaaaaaaat gccacggttg
    29881 catggacttg tggtccggcg cacgcatacc cgcacgcagg atgttttgca gttgttcacc
    29941 cgttggcgcg ggttcagcca ggcgggaggc gctacggcga ttgatcaata gttcgagtgc
    30001 atccatttga ttaactcctg tcgtgatatt tattcacaaa attaacacga gagtggattt
    30061 tgttacagca cagtccgcaa ttcctgctga caagtaccgg ttgggtcatt acgataacca
    30121 catctattgc gcctgtgaca ggtgtgacct taagttggga gaatacatgc gaaccctttg
    30181 gcgatttatt gccggatttt ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat
    30241 ggtacttaac ctgttcttta tttttctcgt actggttggt gtggggatct ggatgcaggt
    30301 cagtggtggt gattcgaaag aaacggccag tcgtggtgca ctgctgctgg acatttctgg
    30361 tgtgatcgtc gataaacccg acagttctca gcggtttagt aaattaagcc gccaactact
    30421 tggtgccagt tccgatcgtc tgcaggaaaa ctcactgttt gatatcgtca acaccattcg
    30481 ccaggcgaag gacgaccgca atatcaccgg tatcgtgatg gatctgaaaa acttcgcagg
    30541 cggcgaccaa ccgtctatgc agtacatcgg caaagccctg aaagagtttc gtgacagcgg
    30601 gaaaccggtt tatgccgtcg gcgagaacta cagccagggg caatattatc tcgccagttt
    30661 cgccaataaa atttggctgt caccgcaagg cgtggtggat ttgcacggtt ttgctaccaa
    30721 cggtctgtac tacaaatcgt tgctggataa gctgaaagtt tccacccatg tgttccgcgt
    30781 gggtacgtat aaatctgccg ttgaaccgtt tattcgtgat gacatgtcac cggcagcccg
    30841 cgaagctgac agccgctgga ttggtgagct gtggcaaaac tatctgaata ctgttgccgc
    30901 taaccgacag atccctgctc agcaggtatt ccctggcgcg caagggttgc ttgagggttt
    30961 aaccaaaacc ggtggcgata ccgcgaaata tgcactggaa aacaagctgg tcgatgcact
    31021 ggcatcgagt gcggaaatcg aaaaagcact gaccaaagag ttcggctgga gtaagactga
    31081 taaaaattat cgcgccatca gttattacga ttacgcattg aaaacgccgg cagataccgg
    31141 tgacagcatc ggtgtcgtct tcgctaatgg cgcaattatg gatggcgagg aaactcaggg
    31201 gaatgttggc ggtgatacca ctgcggcaca aatccgcgac gctcgccttg acccgaaagt
    31261 gaaagcgatt gtcctgcgtg ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat
    31321 tcgcgctgaa ctggcagcag cccgtgcagc gggtaagcct gtggttgtat cgatgggcgg
    31381 catggcggca tctggtggtt actggatttc tacgccagct aattacattg tggctaaccc
    31441 cagcaccctg accggttcta tcggtatctt cggcgtgatc accaccgtag aaaatagtct
    31501 ggattcgatt ggtgttcata ctgatggtgt ctcaacttca ccgctggcag atgtttctat
    31561 caccagggca ctgccgccgg aagcgcagca gatgatgcag ttaagcattg agaatggcta
    31621 taaacgcttt atcacgctgg ttgctgatgc gcgtcattcg acgccggagc agattgataa
    31681 aatcgcccag ggccacgtct ggaccggtca ggatgcaaaa gctaacgggc tggtcgatag
    31741 tctcggggat ttcgatgatg cggttgccaa agcagcagag ctggcaaaag tgaaacagtg
    31801 gcatctggaa tactacgttg atgaaccgac cttcttcgac aaagtgatgg acaacatgtc
    31861 tggttctgtc cgggcaatgt tgccagatgc gttccaggcc atgttacctg caccgctggc
    31921 ctcggtagcc tctaccgtta aaagtgaaag tgacaagctg gccgcgttta acgacccaca
    31981 aaaccgttat gcgttttgcc tcacctgcgc caacgtgcgt taagtcttgt actgagtggc
    32041 cgacagatcg tcggccatat ttttaatcct cttcccgctg tctcgcccca tatttcctta
    32101 tccgcatagt atcaggtgcg ctccccctgc ctcacgtata tacttttgct ctttcgatat
    32161 cattcatatc aatatcatgc aaaagaaatc aatttacgtc gcctacacgg gcgggaccat
    32221 cgggatgcag cgttccgagc agggttatat accggtgtca ggtcatctac aacgccaact
    32281 ggcgctgatg ccggaattcc atcgcccgga gatgccagat ttcaccattc atgaatatac
    32341 gccgctgatg gattcttcag atatgacgcc ggaagactgg cagcatattg ctgaagatat
    32401 taaagcgcac tatgacgact atgatggttt tgtcattctg cacggcaccg atacgatggc
    32461 atataccgcc tctgcgctgt cgttcatgct cgaaaatctc ggtaaaccgg tcattgtgac
    32521 agggtcacaa atcccgctgg ctgagttacg ctctgacgga caaattaatc tgctgaatgc
    32581 gttgtacgtt gcggcgaatt atccgatcaa cgaagtaacg ctctttttca ataaccgatt
    32641 gtatcgcggc aaccgcacta ccaaagccca tgccgatggt tttgatgcgt ttgcctctcc
    32701 aaaccttcct ccgttactgg aagcaggtat ccatattcgt cgtttgaata cgccacccgc
    32761 cccgcacggt gaaggggaat taatcgttca tccaatcacc ccacaaccaa ttggcgtagt
    32821 gacgatttat ccagggattt ctgctgacgt cgtgcgcaat tttctgcgcc aaccggtgaa
    32881 agcattgatt ctgcgctcct atggcgtggg taatgcgcca caaaacaaag ccttcctgca
    32941 ggaattacaa gaagccagcg atcgcggtat tgtggtggtc aacctgacac aatgtatgtc
    33001 cggtaaagtg aatatgggtg gttatgccac cggtaacgcc ctcgcccatg ctggcgttat
    33061 tggcggtgca gatatgactg tagaagccac gctaaccaaa ctgcattacc tgcttagcca
    33121 ggaactggat actgaaacca ttcgcaaggc catgagccaa aacctgcgcg gcgaactgac
    33181 gccagatgat taaggagact gcaatgcccc ctcgcgccct gttactggtc gatttacaaa
    33241 atgatttctg tgctggtggc gcgctcgccg tgccggaagg tgacagtacg gtggatgtcg
    33301 ctaaccgcct gattgactgg tgccagtcgc gcggtgaagc ggttatcgcc agtcaggact
    33361 ggcacccggc gaatcacggc agttttgcca gccagcacgg tgtagagcct tatacgccag
    33421 gccaactcga cggtttgcca caaaccttct ggccagatca ctgtgtgcag aacagtgaag
    33481 gcgcacaatt acatccgtta ctgaaccaaa aagcgatcgc agcggtgttc cataaaggcg
    33541 aaaatccttt agttgacagt tacagtgcct tttttgataa cggccgtcgg cagaaaacct
    33601 ctctcgatga ctggttacgc gatcatgaaa tcgatgaatt gatcgttatg ggcctggcta
    33661 ctgactattg cgtgaagttt accgtgctgg acgcgttaca gttaggttat aaggtaaacg
    33721 tgattaccga tggttgtcgt ggcgtgaata tccagcccca ggacagtgcg cacgcgttta
    33781 tggagatgtc agcagctggg gcaacgctat atacgctggc agactgggaa gagacgcagg
    33841 ggtaatttta cgctggccta caattctgta ctggcattgt aggccaaata aaacacgtca
    33901 gtggcacatc tggcaattga tgccatcaac gaaagattta attcacctca gaaatctctt
    33961 ccaacgacac cttccgcgtt tcgatgccaa aaatggagag aaccagcgca cagagcaata
    34021 acatgacacc aagtaccata aacaccgtga tcgacccata atgtgttaat aatgccgcaa
    34081 cgccataggg cgtgaagact gcgacgatcc gtccgacggc attaacgaaa cccgaaccgc
    34141 gcaggcgtaa atgcgttggc caaagctccg ggatataaac cgccgacgcg aagcaaacat
    34201 acatgtataa aaagaagatc atcaccaggc catagattaa aatcgcccac tctgtagtct
    34261 gaattgaata gatatagcct aacacggcaa taataataag taaggtggag ccaaataacc
    34321 gacgaggaaa atgatcaata attaatgccg caataaatat tcctaccgga gcgccaatca
    34381 taataacagc ggtcattaat attgatttat cgacatcaat gccggagtta acaaatatgg
    34441 tcggtatcca tacggtgatg gtataaagcg aaatgttcat tgcaattaaa acagtaatcg
    34501 cgactaatgt acgtcgtaac atttcacctt taaacaggag ccagaaagta cccttgatta
    34561 ctttaacctt gctgttgctc tgatacgaag taagcggggg taaacgaata ctcttctctc
    34621 tttcaatttg ctgctctact tcacgaagtt ggcattctgc acctgcgatt tgccctttcc
    34681 ctgccagcca tcgtggcgac tcaataaagt atttacctga gagaagccag gctaacagta
    34741 tgccaatacc acccaacata aacattatcc gccaactaaa aaaagcgata accaccacac
    34801 ctatcgccgc agacagcatg ggcgaccagt taccaacaaa tgagagccgc gcggaccatt
    34861 ttccacgcac catcgcgggg ataaactccg taaatgaggc atagccaacc ataatcagcg
    34921 cccccattcc tgttcccatc aggaagcgaa agaagatgag ccagtacata tcagggacaa
    34981 aagcggcccc tgttgcagca ataccgacga tgagaagatt tatgcgaaac gccctgcgcc
    35041 gcccaaagta atcaccgatg aacccaccgg taagagagcc gatgaaataa ccaaacatta
    35101 atgccgaggt aaaggcggca ttgaggaaat tatttgacca tccattgctt accagctttg
    35161 ctaagacgac attaccggag taactcaaaa accctgttaa taacagacta aagcttataa
    35221 taccaaatat acgataatga aaccgggcca aaggcaagcg gtccagtctt gcgccaattt
    35281 gatcatattg ttccattggg tgcctcggat ttgctaaaaa aggtagcaat tgcggcaaat
    35341 tcacttgccg catctttatc atcattttat ttccaggcac aggaacaaat aatattgaca
    35401 acgtgtgcta aaattaccac accagctgga tattattgtc tttgcaaaat gctatccact
    35461 ctgcacccgg tgatttatca gttattatgt aattaatatg actaaagtca gctaactgga
    35521 caaaagcttt gcgatcaaac ttagagtgat caaccaataa cgcaacttct gtcgcctgac
    35581 ggatcattgt ctttttgatt tcagcttccg cttcattaga gtccagcgcg ccgctgttaa
    35641 tatcaagacc tttacaactc attaccatga tatcgacatg atagcggctg atgatctctt
    35701 tagtaattct tccttgcagt gaaagtgtat ttttgtttag ttccccaccc gttgaaacga
    35761 ctttaatttc tgactgagcc agaacatgaa tcgcttccgc cgagttggtt agcaacgtca
    35821 ggtcactacg gtcctgtaac aatttgagca attccattac ggtactactg gaatcggctg
    35881 ccatggtggt tttattgtcg ataaagggta gtgccttgcg tgcaataagc tgcttctctt
    35941 cataaaacga tgaagcgcgc ttataaaaat ggatattctc cgtcaacatc gctgtattta
    36001 aaacagcacc accataggtt ctggtcaaaa agccttcatc ttccagcttc tcaagatcgc
    36061 ggcgaatggt ttcttcggtt acctgaaaaa tcccactcaa atttgagact gtcacctttt
    36121 tatcgttggc aaccatttgc ttaattgcct gaatcctgtc ttttgccgcc acgattacac
    36181 ccctgtatct ttttacatca cattagcgcg attatcgcat aaccgatgtt tactttcaaa
    36241 ataacctgtt tgaatcacag attttcatca cagttttcac agaaacagag gtgaatcgtg
    36301 ttgagtattt aacgctccag ggcctctgcc atttccctca tcaatgttgc gtctgcatcc
    36361 gataagttga tattcagtgc cgcgacattt tcgcgtacct gttgcggtgc agtagcccca
    36421 ctaagaatgg agattaaatc actctgtttt aatatccacg ccagtgccag agtgggaatt
    36481 gtgcactgat aacgagcaca aagtggctgc cactgttcaa gcatatcaat cactttcagc
    36541 atgttttcac gctggaacca gactttattt gcccgagcgc cgcccggaac gtaatcacga
    36601 gtgatggtgc cggtcaacaa tccctgctct agcggggaat aaacctgaac cacaatgcca
    36661 ttatcacgac atagcggcag cagttcgttt tccattgccc ggtcgaggat actgtatttc
    36721 gcctgaataa tatccagttc accatattgc agatactcgc ggatatggtc ggcatcgacg
    36781 ttagcagcgc ctatagcgcg aattttccct tcagccttta actcattaag cactgcgaca
    36841 gtttcagcga tcggcgtata aaatggcggc accgactgcc agtgcgtcat gtagatatcg
    36901 atgtaatcaa tacccagacg ttgcaagctg gcttctacct cttcgcggat agattccggg
    36961 gaaaggtttt tatacaactg ccgatcgcca actttgttga ataaacttcc ttttcgttcc
    37021 cagacaatgc cgcatttggt ttctactaca acctgttcac ggggcaattt ttttaacgcc
    37081 tgaccgacga taacttcact attgccaaag ttatatcctg gtgcagtatc aatcaggtta
    37141 atgccgcaac gatgggcttc aagaatcgta tcaatacata tttgccgatc gagatcgcca
    37201 ttccatgcag gaccgccgcc aatggcccat gtccccaacc ccattcgcga aagcgtaata
    37261 tccgttgtgc ctaaaggtat ctttttcatt tgccttcatt atccttcgta ttcttccagc
    37321 aattgttcga ccagctttct gttttttacg ccggtggtgg caccgacgct tagaaccgag
    37381 atagccgccg ttgcattggc aaagcgtgcg cattcacgca gattttttcc ttctaacagt
    37441 gccgcaataa aacctgaagc aaagttatcg cccgcgccaa tggtgtcgat ggcggttatt
    37501 cctgcgaccg ccggcacctt catcgtcatg tcaccacgct tgataaagca gccgtcttta
    37561 cccgttttaa tcaccaccgt ttttacgccg cacgcaagaa agcagtcagc aatttcatcc
    37621 agtgtctctt tcccggtgag taattttgcc tcggcataat taggaaacag ataatcgaca
    37681 taactcaatg cttcgcaaat atcatccagc gtttcattca accgcggttt gatcatatcg
    37741 gcacagataa tcatctgccg ggctttagct tgcgtaaaaa tttctgttag cgctttacca
    37801 tccaatagtg gactgttgaa aatactggcc agtgataata atttcgcctg ggaaaaccgc
    37861 gcaaaatcaa catcgtcaat attcaatttc cacagactgc cgttacggtt tgtgacaaac
    37921 gtccgctcgc catcctccgt caccagtcca acattaatag aggtatctat gttgacgtcc
    37981 tgcttcaggc tttgaatatc aatattctct ttgcggcaat ggtcgagaat aaactgccct
    38041 gcggcatctt taccaatacg gctcattaat gctgtacgat ggcccagacg agaaataatt
    38101 gttgcttcat ttattgcatc acccccggtg gtcattgcga ttctttcaag aggataagaa
    38161 tccacatcaa agatattttt actgaccggt tgcaatggaa tatcaacaat agcggcacct
    38221 atacaaataa cgtcgagatt atccatatca ttattccgct ttgccatcag aaccgaacag
    38281 tttaattttt tccaatgccc gttcttttac agctttacgt acttcacgtt ctaaatgcag
    38341 gaaaggctga tcctggtttt ctttcactgc gaccattgcg gcctgacaga gctccgtatg
    38401 gatgttgatt ttcgcgatac ccaacgaaat tgcagtttta atgtcggcat cactgatccc
    38461 cgatgcgcca tgcaaaacca gcggaacaga cacggcatcg cgtacgcgtt tgacgacctc
    38521 aaagttcaat tgtggctctg acgtataaac cccatgctgg ttgccgatgg cgacagccaa
    38581 tgagtcacag cccgtacgtt caacaaattc agccgcctgg tcaggatcgg tatagtgata
    38641 gcccgccagc gcttcctcat aaacggtttc attaccgaca tgccctaatt ccgcctctac
    38701 cggaataccc agcggatgga agaaatcgac agcctctttg gttaagcgaa tattttcttc
    38761 gaaatcaaat gcggaagcat cacgcattaa tgaattcata ccatgagtcc aggcgttatg
    38821 aataatctcc atactccgac catgatccca atgagttatt accggcacag ttgcttttcg
    38881 tgccattgat accatcatgt gagagaaatc ttcaaatgag gtgttgccga caaaacctgt
    38941 gccaaaagaa ataataaccg gtgatttcgc ttcttcggct gcgtcgataa cgcccatcaa
    39001 catttctgca ttccatacgt taaaatgggc aattgcataa tgtttatttg tggcatcgtt
    39061 ttcccaatat ctgatatctg cgagcatgtt ctgttctccc gtattaatta atcgctaatt
    39121 ttaataacgc ctttaataat gtcgcgtttg ttgttaaccg actcttcaaa tgcctgttgt
    39181 acatcccgat aatcgtaaat atgcgtcacc atcgatttca catcgaatcg acctgaagaa
    39241 atagcttcaa tcgtaaccgg ataacgattg gcatagcgga ataccgtctg gatagtgact
    39301 tcgcgattta ttttgaggaa attgattgcc gaatcgccgg gtacagtacc aacaatcata
    39361 attttaccgc cgcgcattac cagataaggt gcctgtttaa cggtgaccgc agaacccgct
    39421 gtttcgaaaa caatatctgc gcccatgtct tcggtaaatt gctgacagcg tgcaatagtg
    39481 tcttcttttg cgccgttaat aaccactgtc gcaccaagct gttccgccat tgtcagacgt
    39541 ttttccagca catcaacgac ggcaatttcc gttgctccca ggcatttgca cgcttgcaac
    39601 gtcatcaaac caatacaacc cgctcccaga ataattatct tcttacccgg tttaacatct
    39661 gccagcatcg cggcatgcat cccgactgcg gcaggctcca ccagcgcccc ttccatcgtg
    39721 tccatattgt cgggcagttt gtaagtaaag ctctccggat gacacagata gtgcgttaat
    39781 gcgccgcggt agttgggttg tgtcgccata aaatcaacgt ccgggcagat gttatatttg
    39841 ccttccagac agtaacgaca gtgaccgcaa ggaacgccag gttcgatatt tacccgatcc
    39901 cccggtttaa atttgcgcac gcggcttccc acagccacaa ccgtcccggc gcattcatga
    39961 cccaggccaa tttcttgatt tgggtcttta ggcggaataa acggacctga ttcaaaacca
    40021 tgtacatctg aaccacaaat accgacatat tctactttaa tcaaaacttc atcttcttta
    40081 ggcactggta tttctgctga aataattttc attgtgcccg gcacctgcaa tattgctttt
    40141 gaatttttca ttttgaatcc acccgtatta tttattggct actgcatcaa tacttaaact
    40201 ttcaacggag acacctttgg tttcgattcc aatggtggcg atagcaattg cgacaataat
    40261 tgaaaccgcc cccagaaaaa taaagactcc cgtaacgcca taactactga gcagcactgc
    40321 aacggcataa ggtgcggcaa taccactgat tcgccctacc gcattcgcca gaccggagcc
    40381 acggagtttg gcctctgtcg gccagatctc agggacatac actgccgagg catagcaaac
    40441 gtacatatag acgaaagtaa tcaggaagaa accaatcaac gttatgagca acatgctggt
    40501 ttgcagtgaa tagatatatc cgagcaccgc aatcaggatt aatagcccca cacccattgt
    40561 tttacgcgga attttatcca tcaccagcat ggcaataaaa atgccaaatg gcgcgccaaa
    40621 catactcatg gtatttaaaa caatcgagtc ttttaaatta atcccctggg tcatgaatat
    40681 cgttggcaac cagttaatta gtgtgtactg cacaacgttc atggcaatca gcacacaaga
    40741 gcctaatatc acgcgtttca gtaatactcc tgtcagtaag gctgaatatg gcaccgcttg
    40801 tggtgcttta ccgtcatcag caataaccac gggcggcaaa ggtttacctg tatggcgtat
    40861 gacgccttct tctatactcc gcatgacttt ctcggcttcc tgataccgtc cgcgcgattc
    40921 cagccagcgc ggggattcag gaaagtagcg ccaggccagc gccgtagcga taagcgacaa
    40981 tattgcaggg ataagcagtt gtacccgcca gttccactct gcactaatca gcggcgtgag
    41041 tcccatcgct atcaatgaac agagcggata tgaccagttg ccaataaagg aaacccgact
    41101 tgaccacgtt ccacgatttc taccgggcat atattcggtg aaaccagcaa acagcgtaac
    41161 cagtaaagct cccagcccaa ctcccatcac aaaacggcag gcaataagga aatccatatt
    41221 cggtgagaaa gcaccgacaa ccatcgaggc aatatgaatg gcctcataga ggatgaacgc
    41281 atttctgcga ccggttttgt cgccaatgat gccgcccacc agcgcaccga gaaacattcc
    41341 ggcggtcgtg attgctgaga atgtggctgt ggtggaatta tctgtccagc ccaacgcttt
    41401 cagctgcgcg aggatcaagc caccaacggc attactccag cagacaagca agccaaacgc
    41461 gacgatggca aacattgatg aatgccagcg gcaatccggt aagcgatcca gccgtgcacc
    41521 acaatgcggt tttgttatct gttccatgcg ataacgttcc ttcaggagag tggttattca
    41581 tcaaagtcgt aagtcatgat cactttgatt gcggttttat cgaccatcgc atcaaaccct
    41641 tcgcgccatt gcgacaggcc gatacgatgc gtgatcatcg gtttgacttt gatagcgccg
    41701 ctggctaata gcctgatagc gttacgccat gaggtggagt cataggccat atgcccaatg
    41761 atgcttttgt tccaggcggt aatgtcatta atcgagaaat caagaggttt gaagcccatt
    41821 ccaacgcgta ccacttcccc gttcgggcgg agcatttcga tggcttgttt cagtgcgata
    41881 ttggcaccgg agcattcaat caccagaccc agattgtctt tgccacaaat ttgctggcag
    41941 cgcgccacca catcttcggt agaaccattt actactgccg tcgcacccag ttcttttgca
    42001 accgggaagc ggaccgccac atcttcttgc agaccaacga cgacgatatt taccgccccc
    42061 ataattcgcg ccatttgtac ggagaacagc ccgagtgggc cagtgccgat gacgaccaca
    42121 tcctgaccag gaaggaattt cgattgctgc gcgatggatt tgtaggcatt acagataggg
    42181 tcaagtacgg ctgcgtcctc ataatcaaca ccatcaggga tttcccacaa cgcatgacga
    42241 tgaattttga gtatttcacc aggaaccaga caatatttgg aaaaaccacc gccccaggca
    42301 ttattatcca gaccaaggtt tactttttct gtacaacaca gaaaatcacc ttgttcacag
    42361 gccggacaaa cgccgcagac gtgaccgctg ttatccgaca cgacgcgttg ccccactttc
    42421 cagtctttga ctttttcacc aacctgcgca atacaacctg cgaactcatg gccgcggata
    42481 gagttaaact catcagaacc gctatcgaca ttgtagtgct tcatgtctgc gccgcagatt
    42541 gccgcggctt taatttcaat cactacatct tccgggccac aaatgggttg tggcacatcg
    42601 atcatcttgt agccgccaaa ggctttgcca aaccgagcca gtgctttcat tgcgtcatcc
    42661 tctgtgtttt gttttgctta gatggtcatc atacttaccc acatgatttt tgaggtcaac
    42721 attaaatctg taaaacacag ataatgatct gcgttttaca actcagatca caattacgca
    42781 gaaaatggct ggttctgtta caggttgatg gaaggcggaa cgcaaaatga gcaaatttga
    42841 gagggattac cggccaaata ggtctgagac ttaagttcag gagaagtcat tcaggcgtga
    42901 acgcgacggc aagagaccgg gctggggctt tgcctggtcg tgacgagata attgagatga
    42961 gtcgtggggt tattgttggc tatccccttt ataaccgcgg caaaaagggg acaaaccact
    43021 tacttaaaca tggcaatagg ctttgctgag ataccaaaat cttcttttaa ctgttgtttg
    43081 ctcttcatga ccatctgacc atttgtgtca attgtcatat gctgtgcttc ggtattattg
    43141 cgggcttgcc acagcatcac cagttgcagg cagttctctt tttgttcctc ggtcaacgca
    43201 acgccatcag gccatttccc cagttcaacg gcggtcgaca aacgctggta tacctcaggc
    43261 gtcatgctgt tgataatgtc atcaagattc ataatttttc cgctcctgtg gaataagttg
    43321 ctgaatcgtt ttttcaacca ttgatttctt cgccgttttc gccatcagtg aagcgtaaag
    43381 aagcagagtt aacacaataa cgttcgcccg ttggctgcgg cccgtcgggg aagacatgcc
    43441 ccagatgggc atcacagtta ccgcaacgaa tttctatgcg ctgcattcca tgtgacaagt
    43501 ctttgatata acgaatggat tcttcactca ccggttcgta gaaactgggc cagccacagc
    43561 cggaatcata cttggtttgg gaatgaaaca gcggggcatc gcagatcaaa cagtgatata
    43621 cgccgtcacg cttgttatgc agtaaacgac ccgtaaatgg cggctctgtc ccatgattct
    43681 gcgtcacgta aaactgcatc tcggacaaat tttttttcag ttcttctgcc gaaggtttat
    43741 tagccatttg ctcacatctc actttaatcg tgctcacatt acgtgactga ttctaacaaa
    43801 acattaacac caactggcaa aattttgtcc taaacttgat ctcgacgaaa tggctgcacc
    43861 taaatcgtga tgaaaatcac atttttatcg taattgccct ttaaaattcg gggcgccgac
    43921 cccatgtggt ctcaagccca aaggaagagt gaggcgagtc agtcgcgtta tgcttaggca
    43981 caggattgat ttgtcgcaat gattgacacg attccgcttg acgctgcgta aggtttttgt
    44041 aattttacag gcaacctttt attcactaac aaatagctgg tggaatatat gactatcaaa
    44101 gtaggtatca acggttttgg ccgtatcggt cgcattgttt tccgtgctgc tcagaaacgt
    44161 tctgacatcg agatcgttgc aatcaacgac ctgttagacg ctgattacat ggcatacatg
    44221 ctgaaatatg actccactca cggccgtttc gacggtaccg ttgaagtgaa agacggtcat
    44281 ctgatcgtta acggtaaaaa aatccgtgtt accgctgaac gtgatccggc taacctgaaa
    44341 tgggacgaag ttggtgttga cgttgtcgct gaagcaactg gtctgttcct gactgacgaa
    44401 actgctcgta aacacatcac cgctggtgcg aagaaagtgg ttatgactgg tccgtctaaa
    44461 gacaacactc cgatgttcgt taaaggcgct aacttcgaca aatatgctgg ccaggacatc
    44521 gtttccaacg cttcctgcac caccaactgc ctggctccgc tggctaaagt tatcaacgat
    44581 aacttcggca tcatcgaagg tctgatgacc accgttcacg ctactaccgc tactcagaaa
    44641 accgttgatg gcccgtctca caaagactgg cgcggcggcc gcggcgcttc ccagaacatc
    44701 atcccgtcct ctaccggtgc tgctaaagct gtaggtaaag tactgccaga actgaatggc
    44761 aaactgactg gtatggcgtt ccgcgttccg accccgaacg tatctgtagt tgacctgacc
    44821 gttcgtctgg aaaaagctgc aacttacgag cagatcaaag ctgccgttaa agctgctgct
    44881 gaaggcgaaa tgaaaggcgt tctgggctac accgaagatg acgtagtatc taccgatttc
    44941 aacggcgaag tttgcacttc cgtgttcgat gctaaagctg gtatcgctct gaacgacaac
    45001 ttcgtgaaac tggtatcctg gtacgacaac gaaaccggtt actccaacaa agttctggac
    45061 ctgatcgctc acatctccaa ataagttgag atgacactgt gatctaaaaa gagcgacttc
    45121 ggtcgctctt ttttttacct gataaaatga agttaaagga ctgcgtcatg attaagaaaa
    45181 tttttgccct tccggtcatc gaacaaatct cccctgtcct ctcccgtcgt aaactggatg
    45241 aactggacct cattgtagtc gatcatcccc aggtaaaagc ctcttttgca ttacagggcg
    45301 cacaccttct ctcgtggaaa cctgcgggtg aagaagaagt tctgtggttg agcaacaaca
    45361 caccgttcaa aaatggcgtc gccattcgcg gtggcgtacc ggtttgctgg ccgtggtttg
    45421 gtccggcggc agaacgaggc ctgcctgcgc acggttttgc ccgcaacctg ccgtggacgc
    45481 tgaaatcaca tcgtgaagat gctgatggcg tagcgctgac ttttgaattg acacaaagcg
    45541 aagagacgaa aaaattctgg cctcacgact ttacgctgtt agcgcatttc cgcgtgggta
    45601 aaacctgtga aatcgatctt gaagcacacg gcgaatttga aaccacctct gccctgcata
    45661 cctactttaa cgtgggtgat atcgctaagg taagcgtcag tgggctgggc gatcgcttca
    45721 ttgataaagt aaatgacgcg aaagaagatg tactgaccga cggtattcag accttccctg
    45781 accgtaccga tcgcgtgtat ctgaatccgc aagattgcag cgtgattaat gatgaagcgc
    45841 tgaatcgtat tatcgccgta ggccaccagc atcatctgaa cgttgtaggc tggaacccgg
    45901 gaccggcgct ttcagttagc atgggcgata tgccggacga tggctacaag acctttgttt
    45961 gtgtagaaac ggcttacgct tcagaaacgc aaaaagtgac caaagaaaaa cctgcacatc
    46021 tggcgcaatc cattcgcgtt gcgaaacgtt aatttacgtt aatgttgtgt gccgggtgcg
    46081 atgcatccgg cacgtatcaa catcaaacca tatccagcgc agtttttcct tttggtgccg
    46141 gatatgcttt gtccagcatg gctaattccg ctgacgaaag ttcgacctca agcacagccg
    46201 cattctgttg gacatgggca atcgtggccg cttttggaat cgccatcaca ccctgatggc
    46261 tgattaccca cgccaacaat acttgtgccg cgctgatatt gtgagcatgt gcaatttcgt
    46321 tgactaccgc gttttttaac agtccattgc gcaaccgccc ggcctgggct aacggactgt
    46381 aagccatcac cggcatctgc tgttgctggc accaggggag tagatcgtac tcaatccctc
    46441 gtgaaccgag atggtaaagc acctgattag tggcacactg atttcccccc ggcagctgcc
    46501 agagttcctg catatcagca taatcaaggt tcgaaacgcc ccagcggcgg atttttccct
    46561 gggcgatcaa tttttccatc gctgcgacag tctcttcaaa agcgaaactg ccagaccagt
    46621 gtaataagta gagatcgaga taatcagtat tgagacggcg taaactggct tcgcatgcat
    46681 ttatcgcttt ttgcccgcca gcattccacg gatagacttt agagacgaga aagacgttat
    46741 cacgcagacc ggttaatgct tccccaacca ccttttcggc accaccatcg gcatacattt
    46801 cggcggtatc aatgagggtt aaaccgagct caatgcccgc gcgtagtgca gcaacttctg
    46861 ttttacactt actggcatct tcgcccatat accatgttcc ctgccctatg gctggcagtg
    46921 agacatcgcc actaaattga atcatttttt gttgcattgt ttcctcccag gtattgcacc
    46981 accgtaatgc aaaacagggc gtgacgccct gtttttatgc acaaaatgcc ctggaaagat
    47041 gcattatcag aatttgtagg tgatcccggt agaaatcagg ccagtccagg atttatccac
    47101 catcgggctg tcagtaactt catcagacag acgggtgtag cgcgcggtac cgtaaacact
    47161 ccagtcgccg aggaagttgt agctggcgct cagctccagg taagggctcc agctgtcgtt
    47221 cgggttatag ccacgcagac cgctgcgagc ggactctttg cgcgatacgc cataatagta
    47281 ttcgttctgg ttttcgctgt tccactgcac accaataccc ggagtcacgg tcaggccgcc
    47341 gttggtgtaa cgatacaacc aggccatatc ccagacgatg ccgttgctgt tatccagggt
    47401 atcgccagcc agggtggtac gcaggtaacc gtactgggta aagtgagcat aagacagacc
    47461 agccatcatg gtgctcttac ggtcatccag gtgacgcatt tggtgatcgc cactgtcttt
    47521 agctttgaag taaagcggcg accagtaagc ggtaattgaa agtttatccg ttgcgtcatt
    47581 ccacaggtag taaccaccac ctaagccacg gaaccagaag ttatcgcctt catagttgat
    47641 taccggtact gggtaaacat cggtatcgta atctttatat gggtgttcaa cgacacctac
    47701 gcctgcgccc agggaaaatt taccttcagc gtgcgctacg cctgcagacg ttgcgataag
    47761 cactccaagt gccagaagtt tgagtttggt cacaattaat cattccttaa acaaatgttt
    47821 agcgggcgac aaagtttacc cgtcaataca tcgaaaccca acctttttac gttttcattt
    47881 tttaaagtaa ctgtttaatt ttcctgacgc ggatgacacc gcgcttacag ccaaatgaat
    47941 ttagcgttac tggcgagcct ggtctttaca ttaattatgc aaaatttatg gatgagttgt
    48001 tgatatgcca ttgaaattaa gaaagccgtg taggcaagtt ttccatttgc catctacgct
    48061 taattttgaa ggtgtatcac cgggcacgtt gttctcatcg tcgataaaat ggcatgagag
    48121 ttgctgtgtt ttagcaagag acgtcgttca gtttacctct tccgggagcc tctactattc
    48181 atatgaacgg ctcttaacct gtgctaaaaa acgaaaggac ggcataccat gaatatattc
    48241 gatcactatc gccagcgata tgaagctgcc aaggacgaag agttcacact gcaggagttt
    48301 cttaccactt gtcggcaaga tcgcagtgct tatgccaacg cggctgagcg gctattgatg
    48361 gctatcggtg agcctgtcat ggtcgataca gcccaggaac ccagactttc tcgactcttt
    48421 tctaaccggg tcattgcacg ttatccggcg tttgaagagt tttacggcat ggaagacgcg
    48481 attgaacaga ttgtctctta tctgaaacac gcggctcagg ggctggaaga gaagaaacaa
    48541 atcctgtatc tgctggggcc tgtgggtggg ggtaaatcat cgcttgctga gcgactgaaa
    48601 tcattaatgc agctcgtacc gatttatgta ttgagcgcca acggtgagcg tagcccggtc
    48661 aacgatcatc cgttctgtct tttcaatccg caggaagatg cgcagattct ggaaaaagag
    48721 tatggcattc ctcgccgtta tctcggcacc atcatgtcgc cgtgggcggc aaaacgtctg
    48781 catgaatttg gtggcgatat tactaagttc cgggtagtga aggtctggcc gtccattctg
    48841 caacaaattg ctatcgccaa aacagagccc ggtgatgaga acaaccagga catctccgcg
    48901 ctggtaggga aagtcgatat tcgtaaactc gaacactacg cgcagaatga cccagacgcc
    48961 tatggctatt ccggcgcact gtgccgcgcc aaccagggga tcatggaatt cgttgagatg
    49021 tttaaagcac cgattaaagt gctgcatccc ttgttaaccg ccactcagga aggtaactac
    49081 aacgggacgg aaggtatctc cgccctgccg ttcaacggga ttattcttgc ccactcgaac
    49141 gagtccgaat gggtcacttt ccgtaataac aaaaacaacg aagccttcct cgatcgtgtt
    49201 tacatcgtga aggtgccgta ttgcttgcgc atttccgaag agatcaaaat ctacgagaaa
    49261 ttgcttaatc acagtgaatt gactcacgcc ccatgcgccc ctggcacgct cgaaacactg
    49321 tcacgttttt ccattctttc gcgcctgaaa gagccagaaa actccagcat ttattcaaag
    49381 atgcgggttt atgatggcga aagtctgaaa gacaccgatc ccaaagccaa gtcgtatcag
    49441 gaatatcgtg actacgccgg tgtcgatgaa gggatgaacg gtctgtcgac gcgttttgcg
    49501 tttaagatcc tctcccgcgt gttcaacttc gatcatgtag aagtggcggc aaacccggtc
    49561 catctgttct acgtcctgga acagcagatc gagcgcgagc agttcccaca agagcaggca
    49621 gaacgctatc tggagttcct gaaaggttat ctgatcccga aatatgccga gtttattggc
    49681 aaagagatcc agacggccta ccttgaatcc tattccgaat atgggcaaaa cattttcgac
    49741 cgttatgtta cctacgcgga tttctggatt caggatcagg agtatcgcga tccggatacc
    49801 gggcagctgt ttgaccgcga gtctcttaac gccgagctgg agaaaatcga gaaaccggcg
    49861 gggatcagta atccaaaaga tttccgcaac gagattgtta acttcgtact gcgcgccaga
    49921 gccaataaca gcggacgcaa tccgaactgg accagctatg aaaaactgcg cacggttatc
    49981 gagaagaaaa tgttctccaa taccgaggag ctgttgccgg ttatctcgtt taacgccaaa
    50041 acgtcaaccg acgagcagaa gaaacacgac gactttgtcg accgtatgat ggaaaaaggc
    50101 tacacccgta aacaggtgcg tttactgtgc gaatggtatt tgcgcgtacg taaatcgtct
    50161 taacaaccct ggcccggtac acatgctacc gggcctacaa cgacagcgaa ccgtgggcca
    50221 gagaaatggc aacacaggcg tagcatacag ttggcaaatg tagtacgggg ggcatatgac
    50281 ctggtttatt gaccggcgtc tgaacggcaa aaacaaaagc atggtgaatc gccagcgttt
    50341 tttacgccgt tataaagcgc aaattaaaca gtcgatctcc gaggccatta ataagcgttc
    50401 ggtgactgac gtcgacagcg gcgaatccgt atccattccc acggaagata ttagcgaacc
    50461 gatgtttcat caggggcgtg gcggcctgcg ccaccgcgta catccgggca atgaccattt
    50521 cgtccagaac gaccgaattg aacgtcccca gggtggcggc ggaggttccg gcagtggtca
    50581 gggccaggcc agccaggatg gtgaaggtca ggatgaattc gtctttcaga tttcgaaaga
    50641 tgagtatctt gatctgctct ttgaagattt ggccttaccg aatctgaaac aaaaccaaca
    50701 acgccagctg accgaatata aaacgcatcg ggcgggttat accgctaacg gcgttccggc
    50761 caatatcagc gttgtgcgtt cattgcagaa ctcactggcg cgacgcacag ccatgacggc
    50821 aggcaagcgg cgggaacttc atgcactgga agagaatttg gccatcatca gcaacagtga
    50881 acctgcgcag ctgctggaag aggaacgtct gcgcaaagaa attgcagaat tacgtgccaa
    50941 aattgaacgc gtccctttta ttgacacctt cgatttacgt tacaagaact acgagaagcg
    51001 gcccgatccc tccagccagg cagtgatgtt ttgcctgatg gacgtttccg gttcaatgga
    51061 tcaatccact aaagatatgg ctaagcgttt ttatattctg ctgtatctgt tcctcagcag
    51121 aacgtataag aacgtggaag tcgtatacat ccgccatcat acccaggcga aagaagtcga
    51181 tgaacatgag tttttctact cgcaggaaac tggcggcacc attgtttcca gcgccctgaa
    51241 actgatggat gaggtagtga aagagcgtta taacccggca cagtggaata tttacgctgc
    51301 acaagcatcg gacggcgata actgggccga tgactctccg ctttgccatg aaatcctggc
    51361 gaaaaaatta ttacctgttg ttcgttatta cagctatatc gaaattactc gtcgtgcaca
    51421 tcagacattg tggcgagaat atgagcatct gcaatctact ttcgacaact ttgcgatgca
    51481 gcacatccgc gaccaggatg atatttatcc ggtgttccgt gaactgtttc ataaacaaaa
    51541 tgcaacagct aaagactaaa actatcagcc gggtcattat cgcctggctg atttttagct
    51601 cactgtaaat tatctcattt attacataca ttagcttaca atcgctttaa atatgacagc
    51661 ataaccttta cataat
//
LOCUS       DAZUCE010000008        49547 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZUCE010000008 DAZUCE010000000
VERSION     DAZUCE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.008"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1214
                     /locus_tag="ACHY03_000433"
     CDS             <1..1214
                     /locus_tag="ACHY03_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFS3903712.1"
                     /translation="MTVNDNANDVAVTESYKPIWNWKISQYNVSDIKFETIIKLQFAD
                     RTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIED
                     EVLNVLLRENFNEQLDTNVNTIISILNRRDIILESLQPYLVINKDAVTPCTFLGDQTG
                     DRFSNICGDQFIIDLLKRIMSINDNVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDG
                     IKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINK
                     KHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNG
                     GRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNKNGLM
                     PLIRRRTLLQL"
     gene            1404..1517
                     /gene="yhiY"
                     /locus_tag="ACHY03_000434"
     CDS             1404..1517
                     /gene="yhiY"
                     /locus_tag="ACHY03_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFS3903713.1"
                     /translation="MMTTLLPVFTKPSPLAFNALRAGRICRFLLIPDGIIR"
     gene            1580..2647
                     /locus_tag="ACHY03_000435"
     CDS             1580..2647
                     /locus_tag="ACHY03_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFS3903714.1"
                     /translation="MDKSKRHLAWWVVGLLAVVAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2644..5379
                     /gene="rbbA"
                     /locus_tag="ACHY03_000436"
     CDS             2644..5379
                     /gene="rbbA"
                     /locus_tag="ACHY03_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFS3903715.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEVEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPSSQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5379..6503
                     /locus_tag="ACHY03_000437"
     CDS             5379..6503
                     /locus_tag="ACHY03_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3903716.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6576..6851
                     /locus_tag="ACHY03_000438"
     CDS             6576..6851
                     /locus_tag="ACHY03_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFS3903717.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6848..7207
                     /locus_tag="ACHY03_000439"
     CDS             6848..7207
                     /locus_tag="ACHY03_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFS3903718.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFCGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7257..8117)
                     /locus_tag="ACHY03_000440"
     CDS             complement(7257..8117)
                     /locus_tag="ACHY03_000440"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFS3903719.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(8149..8418)
                     /locus_tag="ACHY03_000441"
     CDS             complement(8149..8418)
                     /locus_tag="ACHY03_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFS3903720.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(8408..9916)
                     /locus_tag="ACHY03_000442"
     CDS             complement(8408..9916)
                     /locus_tag="ACHY03_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFS3903721.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(9909..11267)
                     /locus_tag="ACHY03_000443"
     CDS             complement(9909..11267)
                     /locus_tag="ACHY03_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFS3903722.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNTKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNIIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(11344..11625)
                     /locus_tag="ACHY03_000444"
     CDS             complement(11344..11625)
                     /locus_tag="ACHY03_000444"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS3903723.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKN"
     gene            complement(11622..12095)
                     /locus_tag="ACHY03_000445"
     CDS             complement(11622..12095)
                     /locus_tag="ACHY03_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS3903724.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(12120..12866)
                     /locus_tag="ACHY03_000446"
     CDS             complement(12120..12866)
                     /locus_tag="ACHY03_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS3903725.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(13065..13466)
                     /gene="nikR"
                     /locus_tag="ACHY03_000447"
     CDS             complement(13065..13466)
                     /gene="nikR"
                     /locus_tag="ACHY03_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFS3903726.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQKHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(13472..14278)
                     /gene="nikE"
                     /locus_tag="ACHY03_000448"
     CDS             complement(13472..14278)
                     /gene="nikE"
                     /locus_tag="ACHY03_000448"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFS3903727.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLHAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGEKLTFSSDAGRVLQNAILPAFPVRRRATEKV"
     gene            complement(14275..15039)
                     /gene="nikD"
                     /locus_tag="ACHY03_000449"
     CDS             complement(14275..15039)
                     /gene="nikD"
                     /locus_tag="ACHY03_000449"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFS3903728.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(15039..15872)
                     /gene="nikC"
                     /locus_tag="ACHY03_000450"
     CDS             complement(15039..15872)
                     /gene="nikC"
                     /locus_tag="ACHY03_000450"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFS3903729.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPNAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(15869..16813)
                     /gene="nikB"
                     /locus_tag="ACHY03_000451"
     CDS             complement(15869..16813)
                     /gene="nikB"
                     /locus_tag="ACHY03_000451"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFS3903730.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(16813..18387)
                     /gene="nikA"
                     /locus_tag="ACHY03_000452"
     CDS             complement(16813..18387)
                     /gene="nikA"
                     /locus_tag="ACHY03_000452"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3903731.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLHDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(18498..19085)
                     /gene="acpT"
                     /locus_tag="ACHY03_000453"
     CDS             complement(18498..19085)
                     /gene="acpT"
                     /locus_tag="ACHY03_000453"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFS3903732.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(19087..20316)
                     /locus_tag="ACHY03_000454"
     CDS             complement(19087..20316)
                     /locus_tag="ACHY03_000454"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFS3903733.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(20313..21044)
                     /locus_tag="ACHY03_000455"
     CDS             complement(20313..21044)
                     /locus_tag="ACHY03_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFS3903734.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(21044..21508)
                     /locus_tag="ACHY03_000456"
     CDS             complement(21044..21508)
                     /locus_tag="ACHY03_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFS3903735.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCAAGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(21505..22674)
                     /locus_tag="ACHY03_000457"
     CDS             complement(21505..22674)
                     /locus_tag="ACHY03_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFS3903736.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQIQPQVDDAIAKYGRERIAIVLGTS
                     TSGLHEGDTHVNLCTNGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(22676..23260)
                     /locus_tag="ACHY03_000458"
     CDS             complement(22676..23260)
                     /locus_tag="ACHY03_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFS3903737.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            complement(23257..25575)
                     /locus_tag="ACHY03_000459"
     CDS             complement(23257..25575)
                     /locus_tag="ACHY03_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFS3903738.1"
                     /translation="MTNANVLPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDVKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAQWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKDLKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTVATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITTLTGQSVDQKWFVVYGDSPQQTLRRLEKFTASLEYE
                     KKEGLISNYRTIPLNSLARQEEDLQLLKTAAPTVTKALQNAGLTAVNPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALMQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGFISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(25544..26149)
                     /locus_tag="ACHY03_000460"
     CDS             complement(25544..26149)
                     /locus_tag="ACHY03_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFS3903739.1"
                     /translation="MKFLPLLALLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGK
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFAAQ"
     gene            complement(26146..26568)
                     /locus_tag="ACHY03_000461"
     CDS             complement(26146..26568)
                     /locus_tag="ACHY03_000461"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFS3903740.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(26572..28248)
                     /locus_tag="ACHY03_000462"
     CDS             complement(26572..28248)
                     /locus_tag="ACHY03_000462"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS3903741.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVSEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAERHPAALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKHATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTASRARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVA
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LQLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(28239..28592)
                     /locus_tag="ACHY03_000463"
     CDS             complement(28239..28592)
                     /locus_tag="ACHY03_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFS3903742.1"
                     /translation="MKPHEIERHQAQTNHLEIILHLRADLFWFRGHFAVQPLLPGVAQ
                     IDWAMSYALNLLAPGWRFHSIQNIKFQSPLLPENRVTLALNWQEERQILSFSYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(28579..29940)
                     /locus_tag="ACHY03_000464"
     CDS             complement(28579..29940)
                     /locus_tag="ACHY03_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFS3903743.1"
                     /translation="MKQTLPLARWLTAPRPDDTPIAWLDESTWTLGDLRHDVAQLICR
                     LQQQPGERWALCFENSYLFIVALLATLHAGKTPVLPGHNRVIQLNEQRELFDGVLSDS
                     ELNWQGSLLLVASSPQVATQSFTFAAIAPEAFIELFTSGSTGQPKRVIKPVHLLDREA
                     ELLAERLGARLTGCRVVGSVLPQHLYGLTFRVFLPMALGLPLHAAMLWYVEQFAALSH
                     QHRYIFISSPAFLKRLDTQLSPPPVQVLLSAGGELPWQDVQHTASWLRVWPDEIYGST
                     ETGVIAWRYREQEQRRWLPFPGMQFQAEGDSFRLFSPLMAEDNGLLLDDLLQFSQDGQ
                     FHLMGRRGRIVKIEEKRISLQEVEQRLLALDGIREAAAVPVTRGGRQSIGVLLVLDDE
                     AHLQWQNGGGHSQEMAWRRLLRPTLEPVAIPRYWRVIDEMPVNSMNKRVYAQLQELFH
                     EAP"
     gene            complement(29937..30518)
                     /locus_tag="ACHY03_000465"
     CDS             complement(29937..30518)
                     /locus_tag="ACHY03_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3903744.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFVIWFGLAHNGLHWLLPLIALL
                     LLLRLRQTRRQAGPLQVVTQIVAVVGIALCVASVLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLWSAMPIVERLARLQEPDLPEKGMRYTRRVTQIWCGFFIINGGIALFTALYADMSL
                     WTAWNGMIAYLLMGTLMAGEWLVRRQVMKRDRA"
     gene            complement(30523..30774)
                     /locus_tag="ACHY03_000466"
     CDS             complement(30523..30774)
                     /locus_tag="ACHY03_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS3903745.1"
                     /translation="MTEQQTVYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEA"
     gene            complement(30786..31043)
                     /locus_tag="ACHY03_000467"
     CDS             complement(30786..31043)
                     /locus_tag="ACHY03_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFS3903746.1"
                     /translation="MQALYLEIKNLIISTLNLDELTPDDIDTDAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(31018..31839)
                     /locus_tag="ACHY03_000468"
     CDS             complement(31018..31839)
                     /locus_tag="ACHY03_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFS3903747.1"
                     /translation="MSKLLSRLEWTWRLVMTGLCFALFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCASDLRIVTIGCSEHLLDKQSKWYDVPPDRPFF
                     TVAVHGRVEINKFYDATSQEPALAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(31836..32558)
                     /locus_tag="ACHY03_000469"
     CDS             complement(31836..32558)
                     /locus_tag="ACHY03_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFS3903748.1"
                     /translation="MKFTLNITDWQALAPGLSEAQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLAMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIAAKQPIVSSSLSAGRDTFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPSEMPTWPYAVALVIEAGDEWQCETQSAIAGNETTLPQSMLFLQH
                     YLQNADTFSLPGERVQWRWSRG"
     gene            complement(32599..33657)
                     /locus_tag="ACHY03_000470"
     CDS             complement(32599..33657)
                     /locus_tag="ACHY03_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFS3903749.1"
                     /translation="MYEQDSLSALDAITEAQRIAFAPMLFQTALCLRNAGVLSYLDRQ
                     GKHGATLADITEHSHINEYAASVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWSTIYPALSQLPDAARESWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKYNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKVCIMELFWDAQRFEAASFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(33726..34103)
                     /locus_tag="ACHY03_000471"
     CDS             complement(33726..34103)
                     /locus_tag="ACHY03_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS3903750.1"
                     /translation="MKWFAAIAVVGALAGCARTAPIDQVHSTVSAGHTQEQVKKAILK
                     AGVERKWIMSEAGQGIIKARQQSRDHSAEIRINYTASSYDINYENSQNLQASGGQIHK
                     NYNRWVRNLDKDIQLNLSAGADL"
     gene            complement(34495..35544)
                     /locus_tag="ACHY03_000472"
     CDS             complement(34495..35544)
                     /locus_tag="ACHY03_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS3903751.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            35676..36893
                     /locus_tag="ACHY03_000473"
     CDS             35676..36893
                     /locus_tag="ACHY03_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3903752.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLALPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(36897..37454)
                     /gene="dcrB"
                     /locus_tag="ACHY03_000474"
     CDS             complement(36897..37454)
                     /gene="dcrB"
                     /locus_tag="ACHY03_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFS3903753.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(37527..38192)
                     /locus_tag="ACHY03_000475"
     CDS             complement(37527..38192)
                     /locus_tag="ACHY03_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFS3903754.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            38413..38658
                     /gene="tusA"
                     /locus_tag="ACHY03_000476"
     CDS             38413..38658
                     /gene="tusA"
                     /locus_tag="ACHY03_000476"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFS3903755.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(38760..40958)
                     /gene="zntA"
                     /locus_tag="ACHY03_000477"
     CDS             complement(38760..40958)
                     /gene="zntA"
                     /locus_tag="ACHY03_000477"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFS3903756.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSAEEPQASRLKENLSLITLIVMMVISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATHLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41032..41658)
                     /locus_tag="ACHY03_000478"
     CDS             complement(41032..41658)
                     /locus_tag="ACHY03_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFS3903757.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            41799..42158
                     /locus_tag="ACHY03_000479"
     CDS             41799..42158
                     /locus_tag="ACHY03_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFS3903758.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(42161..42430)
                     /locus_tag="ACHY03_000480"
     CDS             complement(42161..42430)
                     /locus_tag="ACHY03_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFS3903759.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(42420..43016)
                     /gene="rsmD"
                     /locus_tag="ACHY03_000481"
     CDS             complement(42420..43016)
                     /gene="rsmD"
                     /locus_tag="ACHY03_000481"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFS3903760.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            43166..44662
                     /gene="ftsY"
                     /locus_tag="ACHY03_000482"
     CDS             43166..44662
                     /gene="ftsY"
                     /locus_tag="ACHY03_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFS3903761.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAAEVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            44665..45333
                     /gene="ftsE"
                     /locus_tag="ACHY03_000483"
     CDS             44665..45333
                     /gene="ftsE"
                     /locus_tag="ACHY03_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFS3903762.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            45326..46384
                     /gene="ftsX"
                     /locus_tag="ACHY03_000484"
     CDS             45326..46384
                     /gene="ftsX"
                     /locus_tag="ACHY03_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFS3903763.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            46629..47483
                     /gene="rpoH"
                     /locus_tag="ACHY03_000485"
     CDS             46629..47483
                     /gene="rpoH"
                     /locus_tag="ACHY03_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFS3903764.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            47756..48859
                     /gene="livJ"
                     /locus_tag="ACHY03_000486"
     CDS             47756..48859
                     /gene="livJ"
                     /locus_tag="ACHY03_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFS3903765.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(49059..49442)
                     /gene="panM"
                     /locus_tag="ACHY03_000487"
     CDS             complement(49059..49442)
                     /gene="panM"
                     /locus_tag="ACHY03_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFS3903766.1"
                     /translation="MKLTIIRLEKFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTGGALDSLRVREVTRRRGVGQYLLEEVLSDNASVSSWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT        11714 a        13032 c        13467 g        11334 t
ORIGIN      
        1 atatgacagt aaatgataat gctaatgatg ttgcagttac tgagtcgtac aaacccatct
       61 ggaactggaa aatatcgcaa tacaatgtct ctgatattaa attcgaaaca attataaaac
      121 tccagttcgc tgacagaacc tatttctcca attgttcacc tgttgatccc acaagtacca
      181 gaccaaccta ttttggtgat accgatggct cagttggtgc agttttgtac gcgctttttg
      241 caacgggaca tttgggaatc atggctgaag gagaaaattt ccttagccag ttgcttaata
      301 tagaagatga agttctcaac gtattgctaa gggagaactt taatgagcaa cttgatacta
      361 atgttaatac aataatcagt atcctgaaca gaagagacat tattttagaa agtcttcagc
      421 cttatttagt tattaataaa gacgcagtga cgccatgtac gtttttgggt gaccaaaccg
      481 gcgatcgatt tagtaatatc tgtggcgatc aatttatcat cgatttgtta aaacgcataa
      541 tgagcatcaa tgataacgtt catgtattag ctggaaatca tgaaacgaat tgcaatggta
      601 actatatgca aaacttcacg cgcatgaaac cacttgatga ggatacctac gacgggataa
      661 aagattatcc tgtatgtttc tatgacccca aatacaaaat aatggcaaat catcatggga
      721 taacttttga tgatcagcga aaacgttaca ttataggacc gataacggtc tctattgatg
      781 aaatgaccaa tgctctcgat ccggtcgaat tagcggcaat tattaataaa aagcatcatg
      841 ctataattaa tggtaaaaag tttaaaacta gccgggctat ctcttgtcga tcctttaatc
      901 gttatttttc tgtttcgaca gattacagac ctaaacttga agcactatta gcatgctcac
      961 aaatgttagg tataaatcaa gttgttgcac ataacggtaa tggtggaaga gaacgtatag
     1021 gtgaaacggg gacagtttta ggtcttaacg cccgtgacag taaacatgct ggaagaatgt
     1081 tcagcatgca taattgccaa attaaccctg gtgcggggcc tgagataacc actccctgga
     1141 agtcttacca gcatgaaaaa aacaaaaatg gacttatgcc tttgattaga aggcgcacac
     1201 tgttacagct ctgaaccata gacaacatga atattaatgt tactatctgg ttgagactgt
     1261 tgataaaacg taataaggaa tgctctgttc ctgcaactga acaggcgtga aacgctatcc
     1321 aacaggatgg ataccgtttt gtcagccagt tttatagatc acatttatga gtatgcgtgt
     1381 ttcatttacg gccaggaatg ctgatgatga caacattact acccgttttc actaagcctt
     1441 ccccgttggc gttcaatgct ctgcgcgctg gtcgtatttg tcgtttcctt cttatcccgg
     1501 atggaataat ccgctgaacg tcgttcggcg gcagttgtaa cacgcgtcgt ttgcttacag
     1561 ggattatggc gggtcgatta tggataagag taagcgccat ctggcgtggt gggttgtcgg
     1621 attactggcg gtggttgcta tcgtggcgtg gtggctgttg cgcccggcag gtgtgccgga
     1681 aggctttgcc gtcagcaatg ggcgcattga agcgacggaa gtggatatcg ccagcaaaat
     1741 tgccgggcgt atcgacacca ttctggtgaa agaaggccag tttgttcgcg aaggcgaagt
     1801 gctggcgaag atggatactc gcgtgttgca ggagcagcga ctggaagcca tcgcgcaaat
     1861 caaagaggca caaagcgccg ttgccgccgc gcaggctttg ctggagcaac gacaaagcga
     1921 aactcgcgcc gcacagtcgc tggttaatca acgccaggcc gaactggact ccgtggctaa
     1981 acgtcatacg cgttcccgct cgctcgccca acggggggct atttctgcgc aacagctgga
     2041 tgacgatcgc gccgccgctg agagcgccag agcggcgctg gaatcagcaa aagctcaggt
     2101 ttcggcttct aaagccgcta tagaagcggc ccgtaccaat atcattcagg cccaaactcg
     2161 tgtcgaagcg gcacaagcca ctgaacggcg cattgccgca gatatcgatg acagcgaact
     2221 gaaagccccg cgtgacggac gcgtgcagta tcgggttgcc gagccaggcg aagtgctggc
     2281 ggcaggcggt cgggtgctga atatggtcga tctcagcgac gtctatatga ccttcttcct
     2341 gccaaccgaa caggcgggca cgctgaaact gggcggtgaa gcccggctga tcctcgatgc
     2401 cgcgccagat ctgcgtattc ctgcaaccat cagttttgtc gccagtgtcg cccagttcac
     2461 gccaaaaacc gtcgaaacca gcgatgaacg gctgaaactg atgttccgcg tcaaagcgcg
     2521 tatcccaccg gaattactcc agcagcatct ggaatatgtc aaaacgggct tgccgggcgt
     2581 agcgtgggtg cgggtgaatg aagaacttcc gtggcctgac gatctcgtgg tgaggttgcc
     2641 gcaatgacgc atctggaact ggttcccgtc ccgcctgtcg cgcaactggc gggcgtgagc
     2701 cagcattatg gaaaaaccgt tgcgctgaac aatatcacac tcgatattcc ggcccgctgt
     2761 atggtcgggc tgattggtcc ggatggcgtc gggaagtcga gtttgttgtc gttgatttcc
     2821 ggtgcccgcg tcattgagca gggcaatgtg atggtgctgg gcggcgatat gcgcgacccg
     2881 aagcatcgcc gcgacgtctg cccgcgcatt gcctggatgc cgcaagggct gggcaaaaac
     2941 ctctaccaca ccttgtcggt gtatgaaaac gtcgattttt tcgcccgcct gttcggtcac
     3001 gacaaagcgg agcgggaagt gcgaattaat gagctgctga ccagcaccgg gttagcaccg
     3061 tttcgcgatc gtccggcagg gaaactctcc ggcgggatga agcaaaaact cgggctgtgc
     3121 tgtgcgttaa tccacgaccc ggaactgttg attcttgatg agccaacaac gggggttgac
     3181 ccgctctccc gcgcccagtt ctgggatctg atcgacagta ttcgccagcg gcagagcaat
     3241 atgagcgtgc tggtcgccac cgcctatatg gaagaggccg aacgcttcga ctggctggta
     3301 gcgatgaatg ccggagaagt gctggcaact ggcagcgccg aagagctgcg gcagcaaacg
     3361 caaagcgcca cgctggaaga ggcatttatt aatctgttac cgcaagcaca acgccaggcg
     3421 catcaggcgg tagtgatccc accgtatcaa cctgaaaacg cagagattgc catcgaagcg
     3481 cgcgatctga ccatgcgttt tggttccttc gttgccgttg atcacgttaa tttccgcatt
     3541 ccacgcgggg agatttttgg ttttcttggt tcgaacggct gcggtaaatc caccaccatg
     3601 aaaatgctca ccggactgct gcccgccagc gaaggtgagg cgtggctgtt cgggcaaccg
     3661 gttgatccaa aagatatcga tacccgccgt cgggtgggct atatgtcgca ggcgttttcg
     3721 ctctataacg aactcaccgt gcggcaaaac cttgagttac atgcccgttt gtttcacatc
     3781 ccggaagcgg aaattcccgc gcgagtggct gaaatgagcg agcgttttaa actcaacgac
     3841 gttgaagatg ttctgccgga gtcattgccg ctcggcattc gccagcggct ttcgctggcg
     3901 gtggcggtga ttcatcgccc ggaaatgtta atcctcgatg aacctacttc tggcgtcgat
     3961 ccggtggcga gggatatgtt ctggcagttg atggtcgatc tctcgcgcca ggacaaagtg
     4021 actatcttca tctccaccca ctttatgaac gaagcggaac gctgcgaccg tatctcactg
     4081 atgcacgccg gaaaagtgct cgccagcggt acaccgcagg aactggttga gaaacgcgga
     4141 gctgccagtc tggaagaggc atttatcgcc tatttgcagg aagctgcagg gcagagcaac
     4201 gaagtcgaag cgccgcccgt gatacacgac accacccacg cgccgcgcca gggatttagc
     4261 ctgcgccgtc tgtttagcta cagccgccgc gaagcactgg aattgcgccg cgatccggtc
     4321 cgttcgacgc tggcgctgat gggaacggtg atcctgatgc tgataatggg ttacggcatc
     4381 agtatggatg tggaaaacct gcgctttgcg gtgctcgatc gtgaccagac cgtcagtagc
     4441 caggcgtgga cgctcaacct ctccggttcc cgttacttta tcgaacagcc gccgctcacc
     4501 agttatgacg agcttgatcg tcggatgcgt gcgggcgata tcacggtggc aattgagatc
     4561 ccgcccaatt tcgggcgtga tatcgcgcgt ggtacgcctg tggaactcgg tgtctggatc
     4621 gacggagcga tgcccagtcg tgccgaaacg gtaaaaggtt acgtgcaggc tatgcaccag
     4681 agctggctac aggatgtggc gagccgacaa tccacaccct ccagccaaag cgggctgatg
     4741 aacattgaga cgcgctatcg ctataacccg gacgtaaaaa gcctgccagc gattgttccg
     4801 gcggtgatcc cgcttctgct gatgatgatc ccgtcaatgc taagcgccct tagcgtggtg
     4861 cgggaaaaag agctagggtc gattatcaac ctttacgtga ctcccaccac ccgtagcgaa
     4921 tttttgcttg gcaaacagct accgtacatc gcgctgggga tgctgaactt tttcctgctc
     4981 tgcgccttgt cggtgtttgt gtttggcgta ccgcataaag gcagtttcct gacgctcacc
     5041 ctggcggcgc tgctgtatat catcatcgcc accggaatgg ggctgctgat ctccaccttt
     5101 atgaaaagcc agattgccgc cattttcggt acggcgatta tcacgttgat cccagcgaca
     5161 cagttttccg ggatgatcga tccggtagct tcgctggaag ggccgggacg ttggatcggc
     5221 gaggtttacc cgaccagtca ttttctaact atcgcccgcg ggacgttctc gaaagcgctg
     5281 gatctgactg atttgtggca actttttatc ccgttgctga tagccatccc gctggtgatg
     5341 ggcttaagca tcctgctgct gaaaaaacag gagggataat gcgccattta cgcaatattt
     5401 ttaatctggg tatcaaagag ttgcgcagtc tactcggtga taaagcgatg ctgacattga
     5461 ttgtcttctc gtttacggtg tcggtgtatt cgtcagcgac cgttacgcca ggatcgttga
     5521 acctggcacc gatcgccatt gccgatatgg atcaatcgca gttatcgaat cggatcgtta
     5581 acagcttcta tcgcccgtgg tttttgccac cggagatgat caccgccgat gagatggatg
     5641 ccggactgga cgccggacgc tatacctttg cgataaatat tccgcctaat tttcagcgtg
     5701 atgtcctcgc cggacgccag ccggatattc aggtgaacgt cgatgccacg cgcatgagtc
     5761 aggcatttac cggcaatggg tatatccaga atattatcaa cggtgaagtg aacagctttg
     5821 tcgcgcgcta ccgtgataat agcgaaccgt tggtatcgct ggaaacccgg atgcgcttta
     5881 acccgaacct cgatcccgcg tggtttggcg gggtgatggc gatcatcaac aacattacca
     5941 tgctggcgat tgtattgacc ggatcggcgc tgatccgcga gcgtgaacac ggcacggtgg
     6001 aacacttact ggtgatgccg ataacgccgt ttgagatcat gatggcgaag gtctggtcga
     6061 tggggctggt ggtgctggtg gtatcgggat tatcgctggt gctgatggtg aaaggcgtgc
     6121 tgggcgtacc gattgaaggc tcgatcccgc tgtttatgct gggcgtggcg ctcagtctgt
     6181 ttgccaccac gtcaatcggc atttttatgg ggacgatagc gcgttcaatg ccgcaactgg
     6241 ggctgctggt gattctggtg ctgctgccgc tgcaaatgct ttccggtgga tccacgccgc
     6301 gcgaaagtat gccgcagatg gtgcaggaca ttatgctgac catgccgacg acacactttg
     6361 ttagcctcgc gcaggccatt ctctaccgtg gtgcaggatt cgaaatcgtc tggccgcagt
     6421 tcctgacgct gatggcaatt ggcggcgcgt ttttcaccat tgcgctgctg cgattcagga
     6481 agacgattgg gacaatggcg taaggaaggg aaacttgact tcatactgtg atgtcatact
     6541 attgatgtca taaagtgaag tcataaggac atgccatgag aaccaaggta caggctttac
     6601 ggaagaaaca aaaaaataca ttggatcaaa tatttaaaac tcctgttcct caagggatca
     6661 agtggtctga tatagagtca ctggttaaag cattaggcgg agaaattaag gaaggaagag
     6721 gttcgcgttg taagttcata ctaaatatga gcgttgcgtg tttccatcgg cctcatccgt
     6781 cgccagatac cgacaaaggc gctgtagaaa gcgtgcgtga ctggttacta agtatagggg
     6841 taaaaccatg atcaaactga agacgccaaa ctctatggag attgccgggc aacctgctgt
     6901 tatcacttac gtgccagaac tgaatgcatt ttgtggcaaa ttcctgggct tgtcaggtta
     6961 ttgtgatttt gtctctgaca gcattcaggg gctgcaaaaa gaaggggaac tttccctgcg
     7021 agagtatctt gaggattgta aagctgcggg tattgagccg tatgcgcgta cagaaaagat
     7081 taagaccttt acgttacgct atccagaatc gctgagtgaa cgccttaata atgcggctgc
     7141 gcagcaacaa gtctctgtta atacctatat tattgaaaca ctgaatgagc gtttgaatca
     7201 tttataactt atagcggccc aacaatttct gttgcgccgc tatatttttg tggctcttaa
     7261 gatgcctttc taaccaaccg atgcccctca ttcatcatca ggattttgct atacacatct
     7321 tcctcgacgg cgttttttgc tgatgccatg actcgtgcca gattcgcctc gttgtgatta
     7381 ttcacatacg ctttgccgac ggcggtgata aacgctttgc gcagatcgct ggcgatattt
     7441 actttagcga cgcgataatt aacaaagctg cgtaatatct ccggggcgat accggagccg
     7501 ccgtgaataa ccaacggcac cggacagact tcggcaatgc gcttcagcag ggggatatcg
     7561 atacgcggta tatcgtccag gccatgaacg ttgccaatag aaactgccag catgtcgcaa
     7621 ccggtacgct cgacaaaggt cttcaccttt tccggttccg ttttgcaatc ggcttcgctg
     7681 acatgatcat cttctttgcc gagaatcgcc cccagctccg cttcaaccgg tacaccatag
     7741 gatttacaga aatcgacagc ttcctgggtg aaagcgatat tctcttcaaa agagaatgcg
     7801 gcaccgtcaa tcatgaccga ggtaaagccc gcgcgcaccg cctgtttcac atcctcctga
     7861 gttttcccgt gatccagatg aagactgacc ggaacatcca ttttatcggc gtgacgtttc
     7921 acgatttcat aaatgtaatc gtatcccgag agttgtgcat tggttggcgc aatctgaatg
     7981 aagttgggta atcccgaacg ctcaatagcg tttaaaattg aaattgtcgt ctccagattg
     8041 gtggtattaa atgcgcccgc taatacccgt ttttcctgaa tgcggtcgag cagtatccgt
     8101 ccattaacta atggcatatt gaaaacctca tcttttaata tcagttaatt attcagcttc
     8161 accgaagcgg tcgcgaaata agtcagtgag agtctgtatg gcctcgtttt catcatcacc
     8221 gtcgcagcgt atttccaccg tttttccgcc ttttattccg gcgcgcataa tattcatcat
     8281 gctggtggca ttaatatcat taccctcata gtgcagcgtc actttacagc tatatttttt
     8341 ggcggctttc gccagacagg ccgccggacg tgcatgcagg ccagtactgt ttctgacctc
     8401 cacggtttta ttcaacatag tgttcctccc gatgatgtaa tggcatcacg ccttcttgtt
     8461 gtaatttcaa ctgataccag cttttccatt tgtgctgata acgctgtaat tgatggtatc
     8521 tcgcccgatt cggtaacaaa cgttgggcta tagcgactgg cggcggtgga atatccggtg
     8581 cggcggttaa taatgctccg acagaaaccg cctcgttaaa tgcaattgct tcaaccggac
     8641 gctgtaaaat atccgccttt aattgcagcc aaagaggatt atgggttgcc ggaccgatca
     8701 cttttaacgc gctggtgcct ggcacgttaa aacaggcgaa gcagttggca aattccatgg
     8761 ttaatcccag tgatacgctg gcaattaaca tgtcgatact taacgtatcg ccaagcccat
     8821 aaaataatcc acgggtatgg cgatttttat agggcgaacc cgaaccgcga agatgtggaa
     8881 taaagacggg aatgtcatcg agcaaccaat tccccgccag ataatcagca tgtccgcgag
     8941 taagtgatgc ggcgatctcc tcatcggtta gccggaacgt gttgcgcagc cactccagcg
     9001 cgaaaccgcc cacgggcagc gacgcaaaca gggtgaactg gttggcaagt gagtaacagc
     9061 cgtttgccag cttgtcacgt ttggcctgtt catccagcgt cggttgtgta tccagttgca
     9121 gaatgccttc cgtggtcccc gtcgagttaa ggatatcgcc tggcatcatc tgcaacgctc
     9181 gcgcacccac catatggtca tgtccggcca gcgtcacgct gaccggttgc gagaacccaa
     9241 gcgtcttgca aagtgactcg ctgacccatc cggcgtgctc gcctggctgc accaatggcg
     9301 caaatgccgt acagggaacg tgtaacaacg ccgccgcttt cgctgaccat tcgcctttgc
     9361 gcacgtccag acacagcgtg cggctggcta aggtgatatc cgtgcgctgt tcgccagtca
     9421 gccgccagag cagcacttcc ggcgcatgta gccagcgcag gccgcggcgg ttacgcagcg
     9481 ggtaatgttc cagcaaccag cgcattttga aggcagagta attgctgtgt agtggtagac
     9541 cgcagatgtc ataaagtgcc gcactgtctg cctcgctaag cgttgccaga tactcttcac
     9601 cgcgacggtc ataccatgcc agcattggcg tcaggatctc gccatgctcg tcaaggaaca
     9661 cgcctgattc gccaaaactg gcaatgctga tccgtctgac tggcagcggc gaagcggcgt
     9721 tcagttgcgc tatcgcctgg cggacctcct gccagagggc gtcgatatcg aaatcgacat
     9781 cgccctgtgg ggagatctgc tttgccgtca cgaatttatg cgcgcccaac gtcgttgcgt
     9841 ccaggcagga aaagcaggtg actttgcaat tggtggtgcc aatatcgata acgatagctg
     9901 cgctgttatc aggcataaac cgtgctccgc gtcattcgga aaatgccata cagtaccagg
     9961 gcgataaccg gcaccagacc aatggcgatg atattgccgt tgaagatttc cagcagccag
    10021 aagcggaacg ggttaccgcc atcaaggaaa ctggaaacct ggccgctgga gccttcggca
    10081 aatttgaagt taacgtcttt cgccatgccg gtaataaacg gtgcgatttt agtcgcaatc
    10141 cacaggtcgg caataatcac cgggatcgcc gccagaacgg tgcggaaaat attgccacgg
    10201 ctggctaaag caatcatcga cgccattacc gccaggttgg caaggtcgcc aagcggcagg
    10261 acttcattac ctgggaggac aaacgcgata aagaccgaga ttggcgttag cagcagggcg
    10321 gtggagataa tcgccggatt acctacggca acggcgatat ccagtcctat atagagatcg
    10381 tcacggtcag ggtatttggt attgagatac tttttaatgg cttcagacag cggtaataaa
    10441 ccttccatca ggatgcgaac catgcgtggc aggatgaaca tcacaccgcc aatgctgatg
    10501 ccaagcagca acactttttt gaaatcgtat cctgcaatca cgccgagcag tatgcccaga
    10561 atagtgccaa ccatcatcgg ttcgccgaag atgccaaact ttttctgtac ggtttccggg
    10621 tcgatatgaa tacgattgag gccggggata tggtcgatga ttttgtcgac cagcagaccg
    10681 accgggaaga acaccaccga agagagcgtc ggcaatgaga tcccttccag tccaaagtat
    10741 ttttgtacca gtggcgcaga ccagtcggcg agtttaagta ctaccaccgc agcaatcgcc
    10801 gccgccagca aaccaaagaa caggctgccg gtgctgtaat aaaccaacgt accagcaagg
    10861 gcaaaatgcc agtagttcca catatctacg tcaacggtac gggttttatt taacgccagc
    10921 atcgccacgt taatcagaat ggtcagggga ataacaaacg gggcaattgg cgaaccccat
    10981 gtaatagcgg aaagcggcgg ccagcctaaa tctaccaccg gtaaattaat tccggtgcgt
    11041 tcaaccatcg cctgggccgc tggcccgaca ttgctggtca gaatatcgaa aatggcataa
    11101 ataccgacaa aaccaatgcc gactgttaat gacgaacgta aggctttagc gggttttact
    11161 ttaaaacaga gagcaagaat aaataacacc aacggcaaca ttaccgtcgg gccaaatccc
    11221 aatatatatt gcacaatatt atagagggta tgcgcgatat cgttcataat aaacctcttt
    11281 ttcaggcgag agcgcacctc gcgttagcgc ctgatggagt gggttgaaat taattattat
    11341 taattaattt ttaaggatcg aaacaatttg cgccagtact ttctcttcgc caataccggt
    11401 aataatcggc aagccgttaa ctaccgggac gtcgagttca taaggcactt tagtcgttga
    11461 gactaccagt gctacgccat cagtatttcc aggtaaagag gcaacgttgg tttgggaata
    11521 attgacgtta agaccgtgct ctttacaata ttccatgact ttttctgtta ccgccgtgga
    11581 tgtggcaata cccgtggcgc agacaaataa aatggtttga ctcatatttc acctcgttct
    11641 ttcattttgt ttaagattac tgtgtgtatt tctgttgttt ccatcgcaag cagggcttgc
    11701 ataaagggcg tatcctgaat catttccata atccagccca gggcatttaa ttgtttatta
    11761 ctttcgctta aggcgagcaa aaaaatcacc ctgacaggta cggggtcagg atcgccgccc
    11821 atatcggcaa actctaccgg ttccggcagg atgccgacgg caattgcatt gtgcctgaca
    11881 tgtttatggt ccgtgtgcgg aatagcgacg cacggtggtt cggtaggcag ccccgttggg
    11941 tacgtcgcct cacgtttcag tacgtgatcg gcaaaatcag ggacgaccag ctcggcggca
    12001 accaacggtt gcgctaactg acggatgatc tgttcaacgc ttaaccctgc cggaagtcgt
    12061 tgaatataat tttcctgaaa atgtatgcct tgcattttat tcctcttaaa aaagtgtact
    12121 taactcactt cccgccgttg cagtacggtg ccaagataat atttatttcc ttttaaccag
    12181 acgttgccaa actcaatggg taaacctcgg gagaaaaaga ctaactgctc cagatgcaaa
    12241 acgggtgcgt cttcactgat atctaaaaaa tgaccacgtt cattaccaat taatcgcgcg
    12301 gcatagcgac tttccgagta acgaattttt ctttgcgaca aactttctat tgttggaaat
    12361 aaattgtgtt gattaaaatc gatttccgca ataccggggc atagctcaat attgatacgg
    12421 ttctctatca gcatcgcttt ttcatcacca attgagcgta agcgttcaag gtaaagaata
    12481 tcctgaccgg gcgttattct taatttctct gccacataac gattagccgg ttcaatccgt
    12541 gacgtaataa cttcagtggt aaaatgtatt ttctggcttt ccagcgattc cgcgaatgac
    12601 aataatcctt cacctaacgg atatgccacg ttctcttttt tgacaaatgt tcccttaccc
    12661 tggacctgta ttaacacgcc ttcttttacc agttgggcga ccgctttttt aacggttccc
    12721 cgactcactt ccagcatatc cataatctgg tattccgaag gaatgcgatc gtcttccacc
    12781 agatctccgg tataaatact ttctcttatc cactccacca cttgttgata gagcggtatt
    12841 tgtgacgact tacttaatga tttcatggcg cgcacctgaa catttacagt tgtctatgga
    12901 caatcatgga caacttcaag cgtatagtag gtgaacggga gaaaaagagg atcgaagcgt
    12961 ctcacatttt gagggaccaa atcgtgatca aaaaaatgtg acgtgagtcg atatttgtca
    13021 gaagtttact gcgccgtaag gaaataacgg cgcgttggta ggtgttaatc ttccttcggc
    13081 aggcactgca aatgcccgtg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc
    13141 tgcacgtcac ccatgtcgcc tttcaacacg gcgatttcca gacagtcgtc gtgattaatg
    13201 tgcacatgca gcgtggcaac cgatagatcg tggtgatgat gctgggtgga aacaatacgg
    13261 ctggctaagt cgcgtttttc gtgttcatac acatacgaca gcaccgcgaa accttgtgtg
    13321 ccgtgcttct gggtagcttc ctgcgccagg gcgctacgca gaatatcgcg gatagcttcg
    13381 gaacggttgt tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca
    13441 tcaagcgtga tggtgactcg ttgcatttgc gttaaacctt ttctgtggcg cgacggcgca
    13501 cggggaatgc gggtaatatc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta
    13561 atttctctcc caccgcctgg gtttcgacga tttgtccgtt gtccatcacc atcactcgct
    13621 ggcaaaagcg ttccaccagg cgtaagtcat gagtgatgaa caggcaggca gtgccaaact
    13681 gttgttgtag ctttttcagc aggcgaatga cgcccgcctg taacacgaga tcaaggttag
    13741 aaacggcttc atccagaatc agcaatttcg gttcgaccgc cagcgcgcgt gccaggcaga
    13801 cgcgctggag ctggccgccg cttaactgcg gtgggcgctt gtcgagcacg ctgtcgtcga
    13861 gatcgaccgc gtgcagcatt tcgctggcac gcgccagttg ttcggatttt ttcagcgata
    13921 gcaggtggcg catcggttcc cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc
    13981 tgatggagtc ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat
    14041 tgagctttgc caacgattcg ccgcgccagc taatattccc ttgtgaggga gactctaagc
    14101 ctaccagcag ccgcgcgagg gtgcttttcc cgcagccgct gcgccccagc agggcgacgg
    14161 tttcgccgct tttcagggtc agggaaacgt tattaagcac cgcctggtgc tgatgttttc
    14221 cgctaaatcc accgtgcgca tagtgatggg aaaggccgga aacgttaagt aaagtcatga
    14281 tgccagctcc ataccgtaga gggcgagatg agcggaaacc aggctgcgcg tcaccgtatg
    14341 tttgggggcg ttaaacagcg tttcgacatc gccctgttcg acaatcttac cgtgcgacat
    14401 caccgccacg tcatccgcca gacgcgccac tacgcccata tcatgggtga ccagcagcat
    14461 ccccggtgcc tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac
    14521 cacgtcgagg tcggtggttg gttcatcggc gatgataaac ggtgattcgc aaagcaccgc
    14581 catcgcaatc atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag
    14641 cacgcgtgcg gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc
    14701 ggcgggtttc cccagcgcca ggcaagtctc ccgtgcgtgg gtgtgcatgg tgtgcagtgg
    14761 attaaaggcg ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca
    14821 aggcgaaacc ggtttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg
    14881 cagaatgccc agcgtcgcgg cgcaggtcag tgatttcccg ctgccgctac cgccgactaa
    14941 cgctagcaca cgcccgcgtt taagggttaa cgatacgccg tgcaccagcg gctgcgcggc
    15001 ctgtagcgcg atattacgca gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc
    15061 agatgagggt ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg
    15121 ataaacagcg ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc
    15181 gcgtcgttaa tcatcacgcc ccattcggcg gtaggcgcgg tcacgccgag gccgaggaag
    15241 gacatccccg cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc
    15301 gaagggatca ccgcacctgc cagatgatca acaaacactc gcacatggcc cgcgccagaa
    15361 agccgtgatg ccagcacaaa ctcgcgctgg cgcagtgaaa tcaccaggct gcgcaccatg
    15421 cgtgcatacc acgcccagtg cgacagggcg atggcgataa ttacgttggt caacccggtg
    15481 ccgagcacgc cgaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg
    15541 gcgacgcgca tggtagcctg atcaacgcgc ccgccaatca gcccggcgct gccgccaata
    15601 accagcccta atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg
    15661 gtcgctgcca tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc
    15721 tgcgcattcg gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc
    15781 caccactggc tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt
    15841 accgaccagc gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc
    15901 gcgggtccag cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca
    15961 ccatcatcag cgtaaagcac tggatcaccg gatagtcacg gttaaaaatc gccgacaccg
    16021 catagcgccc gacgcctggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca
    16081 gttcgccgat gtgcatcccc acggcagtga tcatcggcag cgaggcatta cgcagaatat
    16141 gacgtcgctc ggtctgtttg tcgttcaggc cgcgcagccg cgcccaggtg acgtgacgct
    16201 gaccggcgac gtccagcata ctggcgcgca gcagccgcgc gttaatcgcc agcgacataa
    16261 aggcaatgga aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta
    16321 gccattgcag atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca
    16381 tcgacacgcc aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc
    16441 gcgccgccca gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg
    16501 caagttccag cgtggcgggc aggaagttca gcatatcgtc cagtaccggg cgttggctgg
    16561 cgaatgagat accaaagtca aggtgcagcg ccttccacaa ccaggtgccg tactggacgt
    16621 acagcggctg atccagtccc agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg
    16681 gcaggttaga cagacgcaaa taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca
    16741 gaaaaatgat caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata
    16801 cgtaacgcaa cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat
    16861 cggcgcgtag gggatgttac ccagctccgg ttttgatacc accatcattg agatgtaact
    16921 gataggcaga taaaccgcct cgtcatgcag acgggtcaga atgtcgcgat acagtgcctg
    16981 acgttgtgct tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt
    17041 gtcggctaat ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag
    17101 gaaggcgtgt gggtcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc
    17161 gtcgcgctga cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc
    17221 aatctggcgc atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc
    17281 gatgaacgac agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc
    17341 aggcagcgtc caaccggctt tttccagcaa cgctttcgct ttctgcggat cgtactggcg
    17401 cggtttcagg ccgaggttgg cgtagggcac agaaggggca aacagggtgt cggcgacctg
    17461 ctgggtgcca tataacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc
    17521 ttcacgtact gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt
    17581 ttcgatcggc tgtgacagtt gggtatggta agccgggttc tggctaaagc gggcgaaggt
    17641 atcgagcggt aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc
    17701 caccgcgcgg gtagtagggt ccgggatgac gttaaaagtg atcttcttaa tcgccggctt
    17761 ttcgccccag tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg
    17821 caaaacccac ggtccggtgc caatcggcgc tttaatcccg ttcatggttt catggttttt
    17881 aaactgcgag ggggcgataa agcggaaagg gcgcggcagg gccagttctt gcaggaaagg
    17941 atagtaggcg cttttcaggg tgatttgcag ctctgtttta ctgagtgctt tgacatcaac
    18001 aatctggttt gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa
    18061 gttttctgcc gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcatg
    18121 cagggtgaag gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg
    18181 gatcaccgaa ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa
    18241 catctggtta ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt
    18301 ggtgatttca tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa
    18361 tagagtgcgg cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa
    18421 atcagtatga cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg
    18481 gcagtcgggt gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc
    18541 gtaaaggggg tgggggtgca gatcgcaagg cttaaatttt ctagctgaca atggctgact
    18601 gacagcgagg agtgataggt gctgtctacg ctgacgattt gccaggcgct gccgccacgc
    18661 tgtttaacga tggcttcttt gcgcgtccag attcgccaga acatttccag ttgctgatca
    18721 ggatgcacgg cgtccatctc agcgtgttcc ccgaggctga atacggcgtt cgccagccag
    18781 cgccagttgg cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcactc
    18841 aacagcaggg cgatatcgtc accgctatgg cttaagttga accatagcgg cgtttccggc
    18901 gcgaatgcag gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc
    18961 gaaagcaatg cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg
    19021 cgtaaacccg gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga
    19081 tacatatcag ggccaacgtt taatgatgat ggatgtatta atgccgccaa aggcaaagtt
    19141 gttactctgc aaaaactcac aatcaacctt gcgggcttca tgcatgatgt aatccagcgc
    19201 gccacaattc gggtctggtt tattcaggtt tagcgtaggc gcaaaccaac cttcgcgcat
    19261 catttgcaga ctcatccagg cttcgagcgc gccacaggca ccaagcgtat gaccaaaata
    19321 acttttcagc gaggagagcg gcacattatc accgtaaatg gttgctgtcg ccagactttc
    19381 tgccatatca ccgcgatcgg tcgccgtacc atgcgctgaa atatagccga tatcttgtgc
    19441 gcttaacccg gcgattttca gcgattgctc catacaatac tgcatggttt cgcgctgcgg
    19501 ctgggtaata tgtgccgcgt cgcagttagt cgcaaagccg acgatttcac cgtaaatggt
    19561 cgcgccgcgc gctttggcgt gctccagttc ttcgagaatg agcgtacctg cgccttcacc
    19621 aatcaccagc ccgtcgcggt tttcatcgaa cggtgaaggt gtggttttcg gcgcatcatt
    19681 gtgctggctg gtggcgaaaa gcgtatcaaa taccgctgct tctgaaggac ataactcttc
    19741 ggcaccacca gcaaccatca ccgtttgata accgtggcga atggcttccc acgcataacc
    19801 gattgcctgg ctgccggaag tacaggcgct ggaggttggg atcacccgcc cgcgcaggcc
    19861 gaaaaagagg ccagtattaa cggcggtggt gtgcggcatc atctgcacat aggtggtgcc
    19921 agtgatgtta ttggtgtgct tttccgtcag catggtggcg aactcgctta ccgggccggt
    19981 actgccggtc gatgaaccgt acgcaatgcc cgtttcgccg ctggttaata tcggatcgcc
    20041 aatcaaccct gcctgctcta acgccagctc gctggcacgg gtcgacattt gcgacacgcg
    20101 gcccatcgca cggatacgct tgcgggtgta gtgctccggt agcgtgaaat catcaacagg
    20161 cgcgccaagc agggtgtgca ggccatcgta gacctgccac tccggcattt tgcgcaccgc
    20221 attttcatac gccagtaagc gtgcagaaac gtcctgccag ttttcaccaa aggcagtcac
    20281 gccgcccatt cctgtaatca ctacgcgacg tgtcataaca tccctccgtt gatggaaata
    20341 acctggcggg tgacgtaacc cgcaatatca gacattaaat aactggcaag cccggcaact
    20401 tcttccgcct ggcccatacg tttcatgggg atcatcgaca ttgcctcttt cagagcgctc
    20461 tcttccattt cgatcatccc ggtatcaata agccccggcg cgatgcagtt gacggtgatt
    20521 ttgcgcttcg cgagttcgat agccagcgct tttgttgcgc cgataattcc ggctttggca
    20581 gcgctgtaat tcacctggcc gcgattgccc attacgccgg aaaccgagga tagcgtgatg
    20641 atgcgcccac cctggcgtgc gccgatcatc ggcataatgc acggctgaat gacgttgtag
    20701 aaactgtcga ggttggtgtg gatcaccgca tcccagtcgt catcgcttaa agccggaaaa
    20761 gcggcatcgc gggcgatccc ggcgttactg accacgccgt accaggcacc gtgttgcgcg
    20821 atctcatgct ccagcacttc ccgacactgt tcgcggtttg cgacgtcgaa actgagcaaa
    20881 cgcccgttac ccccgttggc gacaatggca ttcagcgttt cctgagcgcc tgtggcgtca
    20941 cggtgatagt gaacgccaat gttaaagccg tctgccgcca gttggcaggc gattgcgcga
    21001 ccaatgcctt tgctggcacc agtaaccaga actgaacgac tcatcaggac gctccttgtt
    21061 gaaacagggt ggtaagttct tccgcggtcg gctggaaggt gttaatgcgg cccgttgcct
    21121 cgccgtcgtt gatactgcac tcaaaactgc cgaagcgatc gtcctgcatc agcagtttga
    21181 cggtaatggt cagcgtctgg cctgcgggca gaatgccagc ggcgcaaagc aattcacgcg
    21241 cgcccagtat catccctaac tcaatactgt ttttgccctg ctggtggcga tgccagccag
    21301 accagacgcc gacggtttgc gccatcagtt caagggcaaa ccagccgggg agattgccgt
    21361 ccgggtcgag aaacggtgcc agtacgccac tgggcgaaac cgtcacgcgg cacaccgcgc
    21421 tgtcatcgct gacgctcacc acgtcttcca gcagtaacat gggcgcgtcg tggggcagat
    21481 aagcgccggg ggataaatag tggctcacga aaccctcccg agcagaatgc tggcgttatt
    21541 gccgccaaag gcgaaggaat tggacaggat caccggacgt gccagcggct gcggtttttc
    21601 gataatgccg cagggtggca gcgtgggatc gcgtggcgac aggctgaaat cttgggcggg
    21661 cagcggtaaa tcacgctgta agatcaacat actgattgct gcttcggtaa tgcccgccgc
    21721 gcccagcgtg tggccggtca aatgtttggt ggagctgcac ggtacgcgtt cgccaaacag
    21781 ggcgttaacc accatcgatt caatctggtc gttgagttgc gtggcggtgc cgtgcaggtt
    21841 gatatagccg acgtcatctg gcgtaagctg cgcatcggtc agtgcctgat taatggcgcg
    21901 gatcgcccct tcgccctgcg gatgcggggc cgaaatatgg taagcgtcac tcgattcacc
    21961 aacgcccagc aatgcgatgg gctgcggttc gcgggttaat aacatcagcc ccgcgccttc
    22021 gccaatggtg atacctgcgc ggtcacgacc gaacggctga cataaggttg gcgacagcga
    22081 ttcgaggctg tgaaaaccgt taatcggcat ccggctcagg gtgtctgcgc cacccacaat
    22141 ggcggcatcg accagccccg cctcgataag cctgcgcccg ctgattatcg ctctggcgct
    22201 ggaagagcag gcggttgaaa gggtataggc cgggccgtcg agcgccagcc agtggctgag
    22261 aaagcgcgac ggatcgccga gttcctgttg tgcgtagtgc catgtggtgc tcggctgccc
    22321 gttggtgcac aggttaacgt gcgtgtcgcc ttcatgcagg ccggaggtgc tggtgccaag
    22381 cacaatcgcg atgcgctcgc gaccatattt cgcgatagcg tcatcaacct gcggctgtat
    22441 ctgcgccagc gccgccagca ggatctgatt gttgcgcgag cgatgcgcag cgaatttttc
    22501 cgggataagc ggaagctcgc catccacgcc agccaggacc gcctgcggat gcccctgcaa
    22561 ccagcctgcg cgtgggcgca ttccgggggc gacaccgcga gccaggttgg ctgcgatttc
    22621 gtcaaggtta tttcccaggg cgttgatcat gccgacggcg gaaatataaa tcatatcagt
    22681 cacctaagta ttgaatggtg atgtggtatt taaagacatg ctgctcaatg ctgattggca
    22741 cgcgttttcc ctggcgattc agataggtga tttccgtgac cagtttgccg ctggcgttac
    22801 gcagctcgcg tttgtcaccg ttgtcacgaa gtgtccatcc tgcgggaagt tgcggttgcc
    22861 aggcgctaat cggccagtgg ctgagcatca cgtcagccag tacctgactt gcgggcggta
    22921 actgtgggac gacgatggat tgctcggcgc gtagcccttg cgcatcgtag gtcaccagaa
    22981 acaggcgaat gccgaccgac gacagcccgg caagggtgat tttctgatcg tcggcgttaa
    23041 gcatcactaa cagcgactgg gttttgccgt tgaagctgcc ggtcaacagt tgctgcgaat
    23101 tgactgcggg tgaaatccct ggtgccggca gcgtgatgcg cgtgccaggt tgcagccacg
    23161 cctgcgggcg gccttcctgt tctggttgcg agtggctaca gccggtgagg ataagcgtag
    23221 cggtcagggc gaacgctcgc cagaatgtgg atttgatcat ttttttgttc tctttttatc
    23281 gggcatagcc agcggcgaaa gcaggaaggc ggtgaaaata ccgctcacca gcacaatgcc
    23341 aaaactgctg atggcctggg tggcgctgaa taccaacatt cccagcgtca gcaaggtggt
    23401 gagcattgcc agcgcgatcg ccagtagcga agttaacggt gtaccgcgcg gattactgaa
    23461 aaacagcgtg tagttgatac cgatgccaag caccagcacc agcgccagca gcgaaaagag
    23521 attcaccgcc tgcccgctca tcgccagcac cgccagaccg cagcccagcg aaagcaccga
    23581 aggcaccagg ctgataaacc cttttcgcca gccgaggcgg gccactgcgc cgcaggcaat
    23641 cactgccagc gccaccagca acaagccggt taagacgtag cggtaaagtg cgaacaattc
    23701 atcaaaggtg cttttgcgat caacccaggc aatgccgcaa gggtaatatg tggcgatttc
    23761 ctgcatcaac gcgctacttt taaccccttc aaccggcacc agtacgccgc tttcgccgtt
    23821 ttccagcgtc agccacagca gacgccagcc ttcactggcg gggcttgcca gccattcatc
    23881 gaccttcact ggcatggcgt tgagatccgg gttcactgcc gtcagcccgg cattttgcag
    23941 cgcttttgtt actgtcgggg ccgccgtttt cagcagttgt aaatcttcct cctgccgcgc
    24001 aagggagttc agcggaatgg tgcggtagtt gctgataagc ccctcttttt tctcatactc
    24061 aagcgaggcg gtaaatttct ccagccgccg caatgtttgc tgtggggagt caccgtaaac
    24121 cacgaaccat ttttgatcga cgctctgccc ggtcagggtg gtgatggctt tctcctgggc
    24181 cagaatatgc tgcggtagcg cctgtaactg cgagatatcg tcatcgacgc gcagcattga
    24241 catccccgcc agcgaaaaca gcgccagcgc gacgggcaga ccccgcgaca gttttttatt
    24301 gcgccgccac gctgccagcc agcgcagcat cagcgccatc gccggaaccg ggcgcaccgg
    24361 caggccacgg cacagccacg gatgccagaa caggacggtc agacaagagg cgctcaaccc
    24421 gacggcggca aaaatcgcca tctggcgaat tccggggaag ggggcgagca tcataatcag
    24481 atatgccgcc acggtggtga gcagcgccag tagcaaggca ttgcgcactt tcgccaggct
    24541 ttgccacggc gaaacgtcat tgccgtgcac catccgctcg gtaaggtaat agagcgtgta
    24601 gtcagcggaa atgccaataa cgctcatgct catcaccagc gtcatcaggt gtaattcacc
    24661 gaaaatcaat aacgtggcga ccgttcccgc cagcgcgccg atgccgatgg aaatcacgca
    24721 aagcaacaat gggcgtaaag agcggaatac cgccacaatc agcaatatta cccccagcag
    24781 cgttgccacg cccagggtgg agatatcctg cttcgcctgt tggctggcgt aatcgctgta
    24841 aaacaccgtg ccgcgcgaga gcaactgcgc ctgtgggtaa cgcgttttca gatccttttc
    24901 cagcgtattc agggtcgtga tgagctggtg ggtttgctgc atatcaaacg acgatcccgc
    24961 cagttcaccg tgcagcagat accagtagtt gccctgggga tcctgcgtca ccagccagcc
    25021 gtccatcagt cgtaaacgct ggccgttttt cgccatcgcc agctgcgagc cgcgcattaa
    25081 catcagcgga tcgttttgca gctccttgcc gcttaccccg gagaatgcgg aataaagctg
    25141 ggagaggatc cactgcgcct gcgcttcgcc gccgttttgc aggcgggcgc gggtgtcggg
    25201 gtcaatcagg ccgttgcgat gctgccagaa aaatgctccc cacgcctgct ggctggcggc
    25261 atccattggc cctttaacgt cgccgagcgc agcggatttt tgcagcagcg tcagccactc
    25321 ctgagcgacc ctgggattag cctctttacc ggggctgacc agccacacca gttggcggtc
    25381 cagacgctgc ataaagccgt cattcagcgc tgggggaata tcgcccatcg cctgtttggg
    25441 taacatagcc agcacgctac tgttcagccg tgattgcggc agcaggatca gcaacgccgc
    25501 cgccatgacc aggcagacta gcccccataa cagcgcgggg cgtttactgg gcggcaaaac
    25561 gttggcgttc gtcatcggtc agttgcgctg gcgtcagttg atgttgggta agagcaatat
    25621 cggtgcgatc gccctgttta tcattaagtt gaatgctctc cagataggtt ttcccggcga
    25681 gatcgatggt gttgaaaatt ttatccagcg gcgtggtggt cggcgtcagg cgcagcgtcc
    25741 agcggccttc gcctttgtca gcaaattcga cgcggaagtt ttgttccagc actttgcgat
    25801 cggcctggaa cagcgcgcgc agcaggtggt taaactggaa catctgcggg ttgttttctg
    25861 ccgtgatgat ttgcggcggc tgaccgttga tcacctgcac catgcgttta tcgtccagta
    25921 atagctgcat ggggaatggt gaggtttgat cccacaacag cccctggtcg cgggcgatca
    25981 acatctggcc ctgagatcgc agcggctgcg gcagatcttt aatcgtccgg gtttgatcga
    26041 aatgggcgcg gatcaccggt tgttcggtaa agcgttgctg aagatcgtcc agggtcaggg
    26101 cgctcacaaa cgggctaatc agcagcgcca gcagcggtaa aaatttcatg gtttgactcc
    26161 catgcgttca aacagaatat cggggctgac aaagcacagt tcgcgacttt gctcgtcgac
    26221 cgcgacctga atggtgtatc cggtggtggc gcgtttgccc gtttcggcat cgaaaatctg
    26281 atagccaatc cgcagacggt tttcgaactc ttcgatatgc gcacgcacgc ggatgcgttg
    26341 ctcaaaggtg agggcgtggc gatatttcac ccgcgcatcg accactggcc acaaataacc
    26401 ggactctttc atctgccgat agccgtaatt gaactggttg agcagcgcct cgcgggccac
    26461 ttcaaagtag cgaaaatagt taccgtgcca ggccacgccc atcatatcga cgtcgtgaaa
    26521 gggaatggtc agctcgactt cagcggtaaa tcggggatcg ttaagcaccc tttactcctt
    26581 gtcctgaatt tccggcagtt gccagaaatc gaaaaaatta aaccagtcga ggggcgactg
    26641 gagcgcgtaa tgttccagac gcgcggcgta gtggtcgata gcgttttgca gcgcctgttg
    26701 gcgttcgccg cgcggcagct gcagtgggtc ggcaaaggtt tcgcagtgaa tatgcagctt
    26761 gccgtgctgg cgcagggcga aaatcagatt caccgggcag cgcaaaatag aggcgagaat
    26821 aaacggcccc tgtgggaagg gcgcaggctg gcccataaac ggactccagc agacgcgcca
    26881 gtcgccgccg cgttgcgggg cgacggcgat gcggtcaccg acaatcgcca cccattcgcc
    26941 gttatccagc ttctctttca gcaggatggc ggtttctggg ccgatatctg ttaccggcat
    27001 caggttaatg cctgcctgag gagccatctc ctgcattatc tgtttaaagc gttgggcatt
    27061 ttcgctaaac accagcgcgt taatggtttt gtagccctga atttttgcta gcgcccggca
    27121 cacttccaca tcgccaagat gcgaggccag cagcaatttg ccgcgcggat cgcggacgtc
    27181 aagcgccgct tctgcgcctg gcgcaaacag cacatcacgc cccagttgta gctcgccgcg
    27241 ccagctggcg attttgtcga gcatggcatt accgaaacgt agaaagtgct gatagctgtt
    27301 aagttttgcc gcctgtggtt gatgctgtcg tacacgggcg agccagtctt gcgacgcttt
    27361 gcgcgcacga gaagcagtga gccagtagac gcccaccacc gggtaaagta gcgcgctaaa
    27421 cgccgttctt cccagcagac gccagaccag cagcatcagg cgcattcccc ataatccttt
    27481 cacttcactc tgccgcgccc agtggctgga agagcggcgc atcagcagtg aaggaatacg
    27541 cggcaacatg ccgaaaaaca gacgcgtgtg catgagcgag atgcggacgt tatctttcag
    27601 ggcatcaaaa tgcgaaagcc cgtcttgcgg ataggtgacg cgggtcggca cgaaatagct
    27661 ggtgtttccc tgccagtaga ggcgcaccat cacttcagtg tcgaaatcca tccgcttgcc
    27721 gatggtggcg tgttttgcca gttgcagcgt tggcgcaacc ggataaacgc gaaagccgca
    27781 catgctgtct ttcagttgca gggagagcgt ttcgatccat acccagacgt gggtgaccca
    27841 gcgcccgtac agccgtgagc gggggatgga gtcatcgtaa attggctggc cggagatcag
    27901 cgccgcaggg tgtcgttcag cgagtgccag cagtttgggg atatcttcaa tcgcatgttg
    27961 accgtcagca tccacctgca ccgcatggct gaaccctgcg tctgccgctg cctgtaagcc
    28021 acgcatcacc gccgcgcctt tgcctgcgtt ttctgctaag cgaattaagg tcacgccagg
    28081 ctgttcgcta accagattat ccagttgctg ttgcgtggcg gcatcgctgc cgtcatccac
    28141 cacaatacag ggcagattaa atggcttaag acgcgccagc acgcccggca tcatcgcgcc
    28201 gtggttgtag cagggtatca acacgcaggg agaaaagttt accgacatag gcggatcttc
    28261 ccgctactgg cggtgtggcg ggcgtcaccg tcgtgacgct gatagctaaa gctcagaatc
    28321 tggcgttctt cctgccagtt aagcgcgagc gtgacgcggt tttccggcag caacggagac
    28381 tggaatttta tgttctgaat ggagtgaaaa cgccagccag gcgcgagcag attgagcgcg
    28441 tagctcatcg cccagtcgat ttgcgcaact cctgggagta atggttgcac ggcaaaatga
    28501 ccgcgaaacc agaacagatc cgccctgaga tgcagaataa tttcaaggtg attcgtttgt
    28561 gcctgatggc gctcgatttc atggggcttc atgaaataac tcctgtaatt gcgcatagac
    28621 acgcttgttc atactgttta ctggcatttc atcaataacg cgccagtaac gcggaatagc
    28681 aactggctcc agcgtcgggc gcagtaatcg ccgccaggcc atttcctggc tatgcccgcc
    28741 gccgttctgc cattgcagat gagcttcatc atccagcacc agcaaaacgc caatactctg
    28801 tcgcccgccg cgcgtaacgg gaacagccgc cgcctcgcga atgccgtcca gcgccagtag
    28861 acgttgttct acttcctgga gtgaaatacg tttttcttca atcttaacga tgcgcccacg
    28921 acgccccatc agatggaact gaccgtcctg gctaaactgc aagagatcgt cgagtaacag
    28981 gccgttatct tccgccatca gcggtgaaaa gagacgaaaa gaatcacctt cggcctggaa
    29041 ctgcatacca gggaagggta gccagcggcg ttgctcttgt tcgcggtaac gccaggcgat
    29101 gacaccggtt tccgtgctgc cgtagatttc atcaggccag acgcgtagcc agctcgcggt
    29161 gtgttgtacg tcctgccacg gtagctcacc gccagcggac agaagcacct gaacaggcgg
    29221 cggagaaagc tgggtatcca ggcgtttcaa aaaggccggg ctgctgatga agatatagcg
    29281 atgctgatga ctcaacgcgg caaactgttc gacataccag agcatggcgg cgtggagcgg
    29341 taatcccagc gccattggca ggaaaacgcg aaaagtcagg ccatataaat gctgcggcaa
    29401 tacggaaccg acgacacggc atccggtaag acgcgcgcct aatcgttcag ccagcagttc
    29461 tgcttcacga tccagcaaat gaacaggttt aatcacccgt ttcggctgtc ccgtggaacc
    29521 ggaggtaaac agctcgataa aagcctctgg cgctatagcg gcaaaggtga aggattgtgt
    29581 cgcgacttgt ggggaacttg ctaccaacag caatgaacct tgccagttaa gttcgctgtc
    29641 gctaagcaca ccatcgaaca gttcacgttg ctcgttaagc tggatgacac ggttatgccc
    29701 cggaagcacc ggtgtttttc cggcgtgtag cgtggcgagc agggcaacga taaacagata
    29761 gctgttctca aagcacagcg cccagcgttc acccggctgt tgctgcaagc ggcagataag
    29821 ttgcgcgaca tcgtgacgca aatcgcccag cgtccaggtg ctttcgtcca gccacgcaat
    29881 gggcgtgtca tcagggcgtg gcgcagtcag ccagcgtgca agcggtaagg tctgcttcat
    29941 gcgcgatctc ttttcatcac ctgacggcgc accagccatt caccagccat taatgttccc
    30001 atcaacaaat aagcaatcat gccgttccag gcggtccata acgacatatc ggcataaagc
    30061 gcggtaaaga gggcaattcc accgttgatg atgaaaaagc cgcaccagat ttgcgtgacg
    30121 cggcgggtat agcgcatgcc cttttccggc agatctggct cctgcaaacg cgccagccgc
    30181 tcaactatcg gcattgccga ccacagcgaa ccgccaaaaa ccgccagcat gaccgcgtta
    30241 accactaccg ggtaaaacag cagtagctgg tgcgttttca acagaacgct ggcgacacat
    30301 aatgcgatgc ccacgacagc gacgatttgt gtgacgacct ggagcggccc cgcctggcga
    30361 cgggtctggc gcaggcgcaa caaaagcagc aacgctatta gcggcaacag ccagtgcagg
    30421 ccgttatgcg ctaagccaaa ccaaatcaca aacggccagg cgagcagcaa taatccagtc
    30481 agcagcttaa tcatcggtag cgagcgaatg cccgacatca gatcaggctt cttgtagcag
    30541 gcgttctaca gcctcgacga catcctggac tgtacgcact gctttaaact cttccggctt
    30601 gattttctta ccggttttct tttgcaggtg cacaatcatg tcaacggcgt cgatgctgtc
    30661 cagttccaga tcttcgtaca ggcgcgcttc aggtttgatg tcctgcggat cgatttcaaa
    30721 cagcttaacc agcagggctg agacttcctg atagacggtt tgttgttctg tcatgatggc
    30781 tttcctcagg cacgttgcgc agcaatgaaa gaggccagag tggcgacgga gaaaaagtgc
    30841 tgacgcatct cttcgctttc cgcagagagc acgatgccgt actcattttt tactgccagt
    30901 cccagttcca gggcatcgat agagtccagc cctaaaccgt cgccaaacag cggcgcgtcg
    30961 gtatcgatgt catcaggcgt caactcgtcc agattgaggg tgctgatgat gaggttttta
    31021 atttccagat aaagcgcttg cattatttat tcctgagaga ggtaaacaac cttgttgtaa
    31081 ttgaagcaaa agatgccggt ttaactgcct tgctgccagc gccggttctt gtgaagttgc
    31141 atcgtaaaat ttattgattt caacccgacc atggactgca accgtaaaaa acggccggtc
    31201 aggtggaacg tcataccatt tactttgttt atccagcaga tgctcgctac agccgatcgt
    31261 cacgatgcgt aaatcgctgg cacagcgcac ggcaatattc gccgccccgc gctgcagcgt
    31321 cattttttca ccgggcttcg ttcgcgtgcc ttcggggaaa atcaggatag tgtcaccctg
    31381 tgccagtcgc tgttggcagc ggggtaacag ggtttcagct tcgctgttga ttaaataatc
    31441 tgccgcccgc acgacgccgc caagaaaagg attttttaac agcgcgcttt tcaccaggca
    31501 gtcagtttct ggcatgactg acgccagcag cacgtaatcg atcagcgtgg gatgattggc
    31561 tacgacgagg caaccgcgtt cctgacgtaa aatttcagcg ccatcaatac gataatccag
    31621 cacgccaagc cctttcgcga cagttaaaaa caggcgaaaa ctggcggcaa tgctgcgacg
    31681 cgccaaacga cggcggcggg aagtatccca caccaaaact aacaatatgt taaaccacac
    31741 gacagaaagc agcaggccgc cgagaccaaa cagggcaaaa cataacccgg tcatgaccag
    31801 tcgccaggtc cactccagac gtgacagcag cttactcatc cacggctcca gcgccactgt
    31861 acgcgctcgc caggcagtga aaatgtgtcc gcattttgta aatagtgctg taaaaacaac
    31921 atgctttgcg gcagtgttgt ttcattaccc gcaatggctg actgcgtttc acattgccac
    31981 tcatcgccag cttcaatcac cagcgcgacg gcgtacggcc aggtcggcat ttctgatggc
    32041 agttgtggat gatagaactc aggcagaaag ccatcaaaat cgaccatcaa cacgcgctgg
    32101 tagcccgctt gtaacaggct aagcacttca cacaaacctt gctggaaagt gtcgcgacca
    32161 gcagaaagcg atgacgaaac gatcggctgt ttggccgcaa tggtcaggtt acccacggaa
    32221 gagttgtgta ctgaaagtgc aaagtcggtc ggagaaagcg cctgttcggt tgccagtgca
    32281 tgaacaatgc ggtaattgcg ctccagttcg ccatgacgac tggtatacaa aacggcgtct
    32341 ggctgatggc ggcgtagcat tgccagaccg cattcaacag ccagtttgct gcctgaactg
    32401 aggcggcggg cggtcatcat tggcagttca cttaattttg ccaacggcgc ggcagggtca
    32461 atcgcccacg gctggcgcga ccatgcttgc cattgctgcg cttcgctaag tccaggcgcc
    32521 agtgcctgcc agtcggtaat atttaatgta aatttcatca acgcgcgccc gcggtaaacc
    32581 aaaattatta agtgacgatt atttcttttg gcatatcaaa agtgtatgcc caacgcctaa
    32641 attatcgtgg cgctctgcta cctggaatcc tgctttattc agatagtcgt aaaatttctc
    32701 tgcactatag aaacggctgt taccgtttgc catgcaggta aaataaagtg aggacgcgtt
    32761 caggctaaat gatgctgctt caaaccgctg cgcatcccag aacaactcca tgatgcaaac
    32821 ttttgcgcca ggcttcatta cgctggcgat tttgctgagt atgctaatta tttgttcggg
    32881 tgaaaaacag tctaaaaatt ggctcatcca ccagatatca gcctcgcccg gtaatggggc
    32941 atcgcttagc atatccaccg catggaaatc tatgcgatca gaaaaacctg cattcgcgat
    33001 attttctttc gctagtacga tttgttgtgg cagatctaat aacgtcacgg cgatgttttc
    33061 attgtatttg cagcaacgca aagcccactt acccgtattg ccgcctacat cgtacaatgt
    33121 tgtggggttg ttggcgaata cataaggcag tgcggcgtta aatgcgccat ctgaatagta
    33181 atgatcaaac gcaaaccagc tttcacgagc ggcgtctggt aattgcgaaa gtgccggata
    33241 tatcgtcgac cagtcgccaa atacttttaa cccggaaggt tttccttcat tgagtgaatc
    33301 ggcgaggaag aagagcccct ggtaacagac atcctgggtg aaatccatat tcacgcgagt
    33361 catagtgtca tgcaggaggt aatgaccgat tttcgccagg taataaattc cctctttaca
    33421 ggtaataatt cgcccgctta agcccatatc taacagaacg ctggcagcat attcgttgat
    33481 atggctatgt tcggtgatat cagccagcgt tgcgccatgt ttgccttgcc gatccagata
    33541 tgaaagtaca cccgcattac gtaggcagag tgctgtttga aaaagcatag gggcaaaggc
    33601 aatgcgttga gcttcggtaa tcgcatccag tgcgctaaga gaatcctgtt cgtacatgtt
    33661 gggcacaacc tgttaagagg gaattaaatt attatcggac cgcaacggcc cgactttagg
    33721 aggaattata aatctgcgcc cgctgacaaa ttcagttgaa tatctttgtc gagattgcgg
    33781 acccagcgat tgtagttttt atgaatttgt ccgcctgacg cttgaaggtt ctggctattt
    33841 tcataattga tatcgtagct ggatgccgtg tagttaatgc gtatttccgc tgaatgatcg
    33901 cgtgactgtt gacgtgcttt aattatgcct tgtccggctt cggacataat ccatttgcgt
    33961 tcaacgccag ctttcagaat cgcttttttc acttgttcct gggtatgacc agcgctgacg
    34021 gttgagtgaa cctgatcaat tggcgcagta cgcgcgcaac ccgctaaagc acccacaaca
    34081 gcaatagccg caaaccattt catgtaatgt ctcattagaa ccccattaaa gtattgtttt
    34141 tacgagtaaa gcaagtttta actgtaattg ctggcatcac tattctttat ttagagaaaa
    34201 aattacttgt gagataagtc agtaagtgca catcagagta aaaatctctg aaatgactaa
    34261 attactgcca gcagggacca actataagtg agtgagaaaa atgaggcaat aaaaataaca
    34321 caatgttatc agtgcattct ttgagaagga tttaatcgct attcggcgtt tatatatatt
    34381 tgacttaagg aatggttttt ttaaattaat tttctgaata atgtttcatc atttcctcaa
    34441 atcaaatggc cactgttaga taaacggtgg ccattgcctg ctcaggaggt gctattaacc
    34501 cggtaaccga cttttcggtc tgcccggccc cagtaaaatc gccagtttgc tgccgccttt
    34561 cgtcgtttcc atccagattt tacacacgct ggttaatggc accgaaagta gcatccctac
    34621 cgggccgagc agccatcccc aaattaacaa tgaaagaaat accaccatgg tggacatccc
    34681 caggcgatgg cccatcatcc gtggttctaa aatattgccg atgaccatat ggaccactaa
    34741 aaacaatgcg ccgaccagaa tacattcgta aacaccatta aacagcagca cctgaatcat
    34801 tggcggtacg gcggaaatta ccgcgccgat attgggcacg tagttgagca aaaacgccag
    34861 tactgcccac atcagcgcaa actgcacccc catcagctcc agccccagcc agacgatgac
    34921 acctgtccat aaactgagta gcgtctttaa cgccagataa tgcgaaacgc ctttaagtgc
    34981 gcggtgtagt ccggcgatat gaatctgtgg attattcaat gcaaaacgca ttttgtaagg
    35041 gacgtggcgc acttcaaaca gcataaaaac tacggtcatc accagcaaaa gcacgctcgc
    35101 cattgccccg gaaagcccgg tcattagcgc tgtggtgaag gtaaccactt tttccgagtc
    35161 catccgctgc agcattcgct ccggcgacat atgcaaatta agaaaaggca acatctcctg
    35221 caatttaaaa agtttgcgcg tcagctcctt attaaacttc ggcagcatag agataaattc
    35281 gttaaacgat gccgccagta cgccgaccag cgcggttagt gcgatcagca tcaccaccac
    35341 tacaatcgta atggcaacgg ggcgttgtac tccccgacga ataaaccagg tgacgagcgg
    35401 gttgaggacg atggcaaaaa acagcgccag taacagctgc acaatgatat ctgccgctgc
    35461 gtgaatgccc gcgaggatca ctaccagcga ggccagcttg agcagaatgt gcatgcccgt
    35521 tttatcgggt tgaggggttt ccattggggc ttccttgtga ctttttgtat taagtgtagc
    35581 gggagtcacg ccagcaatat tctgattctt actgctgatt ttcacgtcag cacgtggtaa
    35641 aaatgaaaca ctgttgtaaa aatgtggtga tcctcatgcc cgaacccgta gccgaacccg
    35701 cgctaaacgg attgcgcctg aatttgcgca ttgtctccat tgtcatgttt aacttcgcca
    35761 gctacctcac catcgggttg ccgctcgctg tattaccggg ctatgtccat gatgtgatgg
    35821 gctttagcgc tttctgggca ggattggtta tcagcctgca atattttgcc accttgctga
    35881 gccgtcctca tgccggacgt tacgccgatt tgctgggccc caaaaagatt gtcgtcttcg
    35941 gtttatgcgg ctgctttttg agcggtctgg gatatctgac ggcaggatta accgccagtc
    36001 tgcccgtcat cagcctgtta ttactttgcc tgggacgcgt gatccttggg attgggcaaa
    36061 gttttgccgg aacgggatcg accctgtggg gcgttggcgt ggttggctcg ctgcatatcg
    36121 ggcgggtgat ttcgtggaac ggcattgtca cttatggggc gatggcgatg ggtgcgccgt
    36181 taggcgtcgt gttttatcac tggggcggct tgcaggcgtt agcgttaatc attatgggcg
    36241 tggcgctggt ggccattttg ttggcgctcc cgcgtccgac ggtaaaagcc agtaaaggca
    36301 aaccgctgcc gtttcgcgcg gtgcttgggc gcgtctggct gtacggtatg gcgctggcac
    36361 tggcttccgc cggatttggc gtcatcgcca cctttatcac gctgttttat gacgctaaag
    36421 gttgggacgg tgcggctttc gcgctgacgc tgtttagctg tgcgtttgtc ggtacgcgtt
    36481 tgttattccc taacggcatt aaccgtatcg gcggcttaaa cgtagcgatg atttgcttta
    36541 gcgttgagat tatcggcctg ctgctggttg gcgtggcgac tatgccgtgg atggcgaaaa
    36601 tcggcgtctt actggcgggg gcagggtttt cgctggtatt cccggcattg ggtgtagtgg
    36661 cggtaaaagc ggttccgcag caaaatcagg gggcggcgct ggcaacttac accgtattta
    36721 tggatttatc gcttggcgtg accggaccac tggctgggct ggtgatgagt tgggcgggcg
    36781 tcccggtgat ttatctggcg gcggcgggac tggtcgcaat cgcgttatta ctgacgtggc
    36841 gattaaaaaa acggcctccg gaacacgtcc ctgaggccgc ctcatcatct taaaacttac
    36901 tgaataacca gcgtattaat gatgttttct gcggtggtct gcgctttttg ctgatcgtca
    36961 gctggcagag taatttgcat ggtcagcagt tgattaccca cgttacccag aataacggaa
    37021 gagtacgccg tctggccttt cgcggagata atactgtcta actgctgcat tttgtgacct
    37081 ttcagctcaa tggctttgtt ggtcactact tgcagctgcg gatcgcggct acgttgctga
    37141 tcttccaggc gcttcgccag caccgccaga tcttctttcg gatcatcgcc catgatgacg
    37201 atgactgctt tctgcccggt ggcgtcggac cagacatgca tgttattggc ctgcgttccc
    37261 agcttaccgc tctggtcggt catatccgct ggcagcgaga aacttaactt gccatcaagc
    37321 aggttgacgg gattcccggt agcgttactt tccgcgaccg aaccctgcgc cgtagcgtta
    37381 gtgtctttat catcacaggc cgcaagcccc ataaccagca ggccaattcc gacatattta
    37441 accagattgc gcattgactt cttcctttcg ataaacggcc ataacggctc attcatccat
    37501 cttatcacaa ctctgataac gaacctttaa ctcgcctgca aagcgttgat ttcggattta
    37561 tctgccagtc ttttcaacag catattgagt aatacgccat acattggcag gaagaaaacg
    37621 atactgatta acactttgaa acagtaatcg accagcgcga tttccatcca gtgttcagcc
    37681 ataaaggcat ccgggctacg ccagaaggca atgaagaaaa aggccagcgt gtcgctgacg
    37741 ttaccgaaca gtgtggacgc tgtcggtgcc agccaccagc ggcgactctg acgcaggcgg
    37801 ttaaaaacgt gcacgtcgag gatctgcccc agcgcgtagg ccatgaaact ggcggtggcg
    37861 atacgggcga caaacaggtt gaagtgggcg agtgcgccga atccctgcca ggaacccata
    37921 tagaatagcg acgagatgac gtaggagatc agcaacgccg ggatcatcac cgcaaagatg
    37981 atacgacggg ccagcggtgc gccaaaaata cgcacggtca ggtcggtagc aaggaaaata
    38041 aacgggaagc taaacgcgcc ccaggtggta tggaaaccaa aaatggagac gggaagctga
    38101 accagatagt tactggaggt gatcaccagc agatgaaata acgataacca gaacaacgcc
    38161 ttatagcgtt gagtttgcga gaaaacgttc atattgtacc tttttgatta accattgggg
    38221 tgagggaacc caatacgtac gacacgtctg ttaccttaac ataataacgt gcttaccctt
    38281 ttttcgagcc gccgcatgat actgctttac gttgacaatg caatggttaa ttttcacgca
    38341 atcgttaacc tggtttgctt acggactcac agggcgtaaa ctagcgccgt ttttttatgt
    38401 gatgagaaga aaatgaccga tctcttttcc agccctgacc acacactcga cgcacttggc
    38461 ctgcgctgcc cggaaccagt aatgatggtg cgcaaaacag tgcgcaatat gcagccaggt
    38521 gaaacgttgc tgattattgc tgacgatccg gcgacgaccc gcgatattcc tgggttttgt
    38581 acctttatgg aacacgaact ggttgctaaa gagacggatg gactgcctta tcgttatttg
    38641 attcgtaaag gcggttgatg ggggcagatt ggcttcgatg ccgccttttc ccctcaccct
    38701 aaccctctcc ccagaggaga gaggggaccg atcgcgctca atgttgcgat cggtttgcct
    38761 tatctcctgc gcaacaatct taacgcattc gctgtcacca gcaccgtcgc acccgtatct
    38821 gccagcaccg ccagccatag tccggtcatt cctaacagcg tggtgacgag gaagatccct
    38881 ttcagcccca gcgcaatagt gatgttctgg cggatattgg cgtgagtggc gcgtgccagt
    38941 tcaatcattt gcaccagacc gcgcaggtgg ttatgggtta atgctgcgtc ggcggtttcc
    39001 agcgccacgt ctgtgccgct gcccattgca atcccgatgg cggcagcttt catcgctggc
    39061 gcgtcgttaa taccgtcacc gaccatcgcc agcggcgcat gttgattcag ctcggtcacc
    39121 gctttgactt tatcttccgg caacaggccc gctttaaact ccagccccag ctccccggca
    39181 attgccgccg ctgcgcgtgg attatcgccg gtgaggatca cccctttgac gcccagcgcg
    39241 ttcagttcac tgatggcagt tgcagcatcg gcgcgcaggg tatcctgtaa cgcaatgaca
    39301 ccaagcacgt catcgttacg tactaccagc actaccgttt gcccggcgct ttccagttcg
    39361 ttaattaaac cagcaaatgc atcagcggga tgtttcccgg cagcgcaaat caatacgcgc
    39421 tcaccgttaa cctgcgcttc aatgccagac ccgaccagcg cccgctgtga ttcggcggcg
    39481 ggaatggcga gtgcagcaac ctgtgcttcg cgcacgatgg cctgcgccag tggatgcgtc
    39541 gcgccttgct cgaccgccgc cgccagtgtc agcagttcag attcactaat acccgttgcc
    39601 ggatgaatcg cggtaacgcg cggtttaccg acggtcagcg taccggtttt atcaaacgcc
    39661 acctgggtaa cacgacctag ctgctccagc gccgcaccgc ctttaattaa cgccccacga
    39721 cgtgccgctg ctgccagccc ggaggtaatc gccgcgggcg tggagataac taacgcacac
    39781 gggcagccaa tcagcagcag cgtcagccct ttataaatcc actcctgcca gcttgcggca
    39841 aacagcagcg gcggcaccag cgttaccagc agggcgacgg ccataatcgc tggcgtataa
    39901 atacggctga aacggtcgat aaaccgctca atgggcgcac ggcgctcttc ggcttcttcg
    39961 atcagtttca gaatccggtc aatggcgctg gcacccggtt ctgacagcac ttccagcgtt
    40021 accagacggt ctacgctggt ggctcctgca ggaactttat cgcccgttgc gcgctccacc
    40081 ggaatggatt cgccggtcag ggcgctttca tcaaaactgg caaacggtga gagcagttta
    40141 ccgtcggcag gcaaacgtcc acctgcggcg acttcaatca catctccagg gcgcaggctg
    40201 ttaatcgcca cctcttcccg ctcaccgtta cgcaggtgcg tggcggtttc tggtttcagc
    40261 gccattaacg cgctaacgcc ctgacgcgcg cggctggcgg cccagccttc cagtcgttca
    40321 ccaatcaaaa acagcagcaa caccatcgca gcttcagccg ttgcgccaat aaacagcgca
    40381 ccaatagcag ctacgctcat taaggtttca atggcgaagt aactgcctga tttgatcagc
    40441 cgtaatgcct gacgagcaat cgggtacagc ccaaccagcg tggtcgcgat aaacgccagt
    40501 tgcccgaacg gatgattgaa ctgctccaga ccccagctga ttaccatcat cacgattagc
    40561 gtaatcagcg acaaattctc tttcaggcgt gatgcttgcg gttcttcggc ggactgttca
    40621 tcgcgcaggg aatagcccgc tttttgcacc gcagattcaa cttgtgcacg gatgtcattg
    40681 tcggcatcga ccaccagttt ttcggtggcg aacagtacct gcacctgatt cacgcctgca
    40741 agctggcgca cggcattttc taccttgcgc gcacaggcgg cgcagtccat gccgctgact
    40801 ttccagctat agcgggtgcc ggagacgttt tcagagagag ttggcgtgct ggaacatgcg
    40861 ccgtcgcagc aacagtcgtt ggcgttctgt accgtggtta gcggtttgaa cgcagcaaat
    40921 tgaggggctt tcttgccgtg attgtcagga gtcgacatgg catcctccgg ttaagttttt
    40981 tctcattaac cgaaggatac actctggagt cgactccaga gtcaagtttt atcagagata
    41041 cagcgagcgg acgatgagga agtgaccggc aaagtagcag gccgcagcaa tcgcgttatc
    41101 cgcacggaag cgtcggcgat agtggctacc cagccagaca aagttactga taaacagcaa
    41161 tgaagcgccg acaaacgcag agagtgccgg agcggtcgga cggaagaacc acagttcacc
    41221 tgccagccac accatcacca gcgtcatgcc gataaacgtg cagatgggcc aacgatactc
    41281 ttccagccgc gtccagataa tcgccagtaa cagcgcgccc agaaccagca acaccagtgg
    41341 cagaggccag aagaaagaga gcgtcatctg actggcgaaa tagatggtgt acagcaggtg
    41401 cgagaggaaa aacgcgccga tggcgtacat cagacgttga cgtggcaaca gggttagcgc
    41461 atcgcccagc agtgaggcgc acagccccgc cagcaccaga tagctaatgg cgtcgaacat
    41521 cggcgcttgc caggccagta acaacaggag aagaagggtt aacggtttaa acacccagcg
    41581 ttgccaggtt ggcccacgat acgatgcatc cacagatagc catgcggaaa gacagacagc
    41641 gataaacgac caaagcatct tagttccttg aattcgttat tttttcgtag gcggaatgcc
    41701 tacggtcttg cttccagttt agtggcgagg gtggacggat gacaacacca taactggcag
    41761 ggtatgctta attccgcatt ttccgatgag ggataaagat gagcaaacca cctcttttct
    41821 ttattgttat cattggctta atcatcgtcg ccgcatcgtt tcgttttatg caacagcgac
    41881 gggaaaaagc tgataatgat atggcaccgc tccagcaaaa gctggtggtg gtgagcaaca
    41941 agcgggaaaa accgattaac gatcgccgtt cgcgtcagca ggaagtgact ccggcaggca
    42001 ccagtatgcg ctatgaggca agcttcaaac cgcaaagcgg aggaatggag cagacgtttc
    42061 gcctcgacgc ccagcagtac cacgccctga cagtgggcga taaaggtacg ctgagctata
    42121 aaggaacgcg ctttgttagc tttgttggcg aacaataagg ttatttttta actttaaatt
    42181 tcttctgcca gaccagcagt tcaaacacgc cgaaaaggaa aatccgcacc ttttcggcgg
    42241 tggtcatctg cgggccatct ttcggtaaag tggatttcaa tagcgccagc tgcataccat
    42301 gcatcagcac ggtaaaaatc agcgccacgt taacgaaaat attcagcggg cgcgggaagg
    42361 gatgcaccag gttgaggatt aaaaatcccc aaacgcagag cattaacaaa cgtccaatat
    42421 taatcagcat cactttctcc ttgtgcttcg cgttgataca gccgataagc cacctgaccc
    42481 gccacttttt cccgatgcaa tgaccagttt gccggaacag tgggcagacc gttttcgact
    42541 tcgctttcga cataaatcag ggcttcgtca gccagccagc cgttatcttc cagtaaattt
    42601 atcgtctctt ctaacaagcc acggcggaac ggtggatcga caaacacgat attatgcggt
    42661 gtgccttttt gcgccaggaa tgacatcgcg ttgctgttca ccacgcgtgc attgcctgct
    42721 tttagtgtcg ccagattctt aattaactgc tgagaaactg cacgatccat ctcaatcaac
    42781 gtggcccccg cagcgtagcg tgataacgct tccagcccca gcgcgccgct tccggcgaag
    42841 caatccagac attgggcgtc aacaatgacc ggagccagcc agttaaataa cgtttcgcgt
    42901 acgcggtcgg tggtggggcg cagacctggg ctatcaggaa ccgggagttt acggcctcgc
    42961 cactgcccgc caataatgcg gatttggccg ctgccggaat gattcggttt tttcatgata
    43021 aattgctcaa tccgccagat aacacatact tgcaggcggt gatgtgaatt aagttaagtg
    43081 atagactatt tcatcatttt tttagctgct atgtacatag cgttaacgct gtgccatgaa
    43141 gcaacagcga ggagtgtagt cgcaaatggc gaaagaaaaa aaacgtggct ttttttcctg
    43201 gctgggcttt ggtcaaaaag agcagacccc ggaaaaagag acagaagttc agaatgaaca
    43261 accggttgta gaagaaatcg ttcaggcgca agagcctgtg aaggcttctg aacaagccgt
    43321 tgaagagcag ccgcaggcgc atactgaagc cgaggcggaa acttttgctg ccgaagttgt
    43381 ggaagtcact gaacaggttg ctgaaagtga aaaagcgcag cctgaagcgg aagtcgttgc
    43441 acagccggaa ccggtcgtag aagaaacgcc ggagccagtg gctatcgaac gtgaagagct
    43501 gccgttgccg gaagacgtca acgccgaaga ggtttcgcca gaagagtggc aggctgaagc
    43561 ggaaaccgta gagattgtcg aagcggcgga agaagaagcg gctaaagaag aaattaccga
    43621 cgaagagctg gaaacacagg cgctggctgc cgaagcggca gaagaggcgg tgatggtggt
    43681 tcctccggca gaagaagagc agccggtgga agaaatcgct caggagcagg aaaaaccgac
    43741 caaagaaggt tttttcgcgc gcctgaaacg cagcctgtta aaaaccaaag aaaatctcgg
    43801 ttccggattt atcagcctgt tccgcggtaa aaaaatcgac gatgatctgt ttgaggagct
    43861 ggaagagcag cttttgatcg ccgatgtggg tgtggaaacc acacgtaaaa ttatcaccaa
    43921 tctgacggaa ggcgcatccc gcaagcagct tcgtgatgcc gaggcgctct atggcctgct
    43981 gaaagaagag atgggcgaga ttctggcgaa agtcgatgag ccgctgaatg ttgaaggtaa
    44041 aacgccgttc gtgatcctga tggtgggcgt caacggtgtg ggtaaaacca cgacgattgg
    44101 taagctggcg cgtcagtttg agcagcaggg taaatcggtg atgctggcgg cgggcgatac
    44161 tttccgtgca gcagcggttg aacagcttca ggtctggggt cagcgcaaca atattccggt
    44221 tattgcccag cataccggtg cggattccgc ctctgttatc ttcgacgcca ttcaggcagc
    44281 taaagcgcgt aatatcgacg tcttgattgc cgataccgcc ggacgcctgc agaacaaatc
    44341 gcacctgatg gaagagttga agaaaatcgt ccgcgtgatg aagaaactcg acgttgaagc
    44401 gccgcatgaa gttatgctga ctatcgatgc cagcaccggg cagaacgcgg taagccaggc
    44461 caaactgttc cacgaagccg ttggcttaac cggcatcacg ctaacgaaac tggacggcac
    44521 ggcgaaaggc ggggtaattt tctcggtggc tgaccagttt ggtatcccta tccgctacat
    44581 tggtgtcggc gaacgtattg aggatttgcg tccgtttaag gcggacgact ttatagaggc
    44641 actttttgcc cgagaggatt aacaatgatt cgctttgaac atgtcagcaa ggcttatctc
    44701 ggtgggagac aggcgctgca gggcgttacg ttccatatgc agccgggtga gatggcgttt
    44761 ctgaccggtc attccggcgc agggaaaagt accctcctga agctgatctg tgggattgag
    44821 cggcccagcg ccgggaaaat ctggtttagc ggccatgaca tcacgcgtct gaaaaaccgt
    44881 gaagttccgt ttctgcgccg ccagattggc atgattttcc aggatcacca tctactgatg
    44941 gaccgtactg tctacgataa cgtggcgatc ccgctgatta tcgctggtgc cagcggtgac
    45001 gatattcgtc gccgggtgtc ggcggcactg gataaagtcg ggctactgga caaagcgaag
    45061 aacttcccta ttcagctttc cggcggtgaa caacagcgtg ttggcattgc ccgcgcggtg
    45121 gtgaacaagc ctgcggtact gctggcggac gaaccgactg gtaacctgga cgacgcgttg
    45181 tcggaaggga ttctacgtct gtttgaagag tttaaccgcg ttggggtaac cgtattgatg
    45241 gcaacgcacg acatcaacct gatttcgcgg cgttcctatc gcatgctcac cctgagcgat
    45301 ggtcacttgc atggaggcgt gggccatgaa taagcgcgat gcaatcaatc atattcggca
    45361 gtttggcggg cgtcttgatc gcttccgtaa atcggtcggc ggctcaggcg acggcggtcg
    45421 taacgcaccg aaacgcgcga aatcctcgcc aaaaccggta aatcgcaaaa ccaacgtttt
    45481 caacgaacag gtgcgctatg ccttccacgg cgcattgcag gatctgaaaa gcaaaccgtt
    45541 cgccacgttt ttaacggtga tggttatcgc catctccctg acgctgccca gcgtctgtta
    45601 tatggtgtac aaaaacgtta accaggcggc gacgcaatat tatccgtcac cgcaaatcac
    45661 tgtttatctg caaaaaacgc tggacgatga cgctgctgcg ggcgtggtgg cacagttgca
    45721 ggccgagcaa ggcgtggaga aagtgaatta tctttctcgt gaagatgcac tgggcgagtt
    45781 ccgtaactgg tctggttttg gtggtgcgct ggatatgctg gaagaaaacc cgcttccggc
    45841 agtggcggtg gtgatcccga aactcgactt tcaggggacg gagtctctga atacgctgcg
    45901 tgatcgtatc acgcagatta acggcattga cgaagtgcgg atggatgaca gctggtttgc
    45961 ccgtctggcg gcgttgaccg ggctggtcgg gcgcgtttcg gcgatgatcg gcgtgttgat
    46021 ggtggcggca gtgttcctcg tcatcggtaa cagtgtacgc ctgagtatct ttgctcgtcg
    46081 tgactccatt aacgtgcaga aactgattgg tgcgacggat ggtttcatcc tgcgcccgtt
    46141 cctgtacggt ggcgcactgc tgggattttc tggcgcattg ttgtcactaa ttttgtcaga
    46201 aattctggta ctgcgtttgt catcggcggt tgcggaagtg gcacaggttt tcggaacgaa
    46261 gtttgatatc aatggcttat cgttcgatga atgcctgcta ttgctgctgg tatgctcgat
    46321 gattggctgg gtggcagcgt ggcttgccac ggtacaacat ttacgccact ttacgcctga
    46381 ataataaaag cgtgttatac tctttccctg caatgggttc cgtagcaggg aaagagaccc
    46441 cgttgtctct tcccggtatt tcatctctat gtcacatttt gtgcgtaatt tattcacaag
    46501 cttgcattga acttgtggat aaaatcacgg tctgataaaa cagtgaatga taacctcgtt
    46561 gctcttaagc tctggcacag ttgttgctac cactgaagcg ccagaagata tcgattgaga
    46621 ggatttgaat gactgacaaa atgcaaagtt tagctttagc cccagttggc aacctggatt
    46681 cctacatccg ggcagctaac gcgtggccga tgttgtcggc tgacgaggag cgggcgctgg
    46741 ctgaaaagct gcattaccat ggcgatctgg aagcagctaa aacgctgatc ctgtctcacc
    46801 tgcggtttgt tgttcatatt gctcgtaatt atgcgggcta tggcctgcca caggcggatt
    46861 tgattcagga aggtaacatc ggcctgatga aagcagtgcg ccgtttcaac ccggaagtgg
    46921 gtgtgcgcct ggtctccttc gccgttcact ggatcaaagc agagatccac gaatacgttc
    46981 tgcgtaactg gcgtatcgtc aaagttgcga ccaccaaagc gcagcgcaaa ctgttcttca
    47041 acctgcgtaa aaccaagcag cgtttgggct ggtttaacca ggatgaagtc gaaatggtgg
    47101 cccgtgaact gggcgtaacc agcaaagacg tacgtgagat ggaatcacgt atggcggcac
    47161 aggacatgac ctttgacctg tcttccgacg acgattccga cagccagccg atggcaccgg
    47221 tgctctatct gcaggataaa tcatctaact ttgccgacgg cattgaagat gataactggg
    47281 aagagcaggc ggcaaaccgc ctgaccgacg cgatgcaagg tctggacgag cgtagccagg
    47341 acatcatccg cgcgcgctgg ctggacgaag acaacaagtc cacgttgcag gaactggctg
    47401 accgttacgg tgtttccgct gagcgtgtgc gccagctgga aaagaacgcg atgaaaaaac
    47461 ttcgcgccgc cattgaagcg taatttccgc tattaagcag agaaccctgg atgagagtcc
    47521 ggggtttttg tttttttggg cctctgcaat aatcaatttc cccttccggc aaaacgccaa
    47581 tccccacgca gattgttaat aaactgtcaa aatagctatt ccaatatcat aaaaatgggg
    47641 tatgttttag cagagtatgc tgctaaagca cgggtagtca tgcataaaac gaaataaagt
    47701 gctgaatcac aacaccacaa cacacgtaat aaccagaaga atggggattc tcaggatgaa
    47761 tataaagggt aaagcgttac tggcaggatg tatcgcgctg gcattcagca atatggctct
    47821 ggcggaagat attaaagttg cggtcgtggg cgcaatgtcc ggtccggttg cgcagtacgg
    47881 tgaccaggag tttaccggcg cagagcaggc ggttgcggat atcaacgcta aaggcggcat
    47941 taaaggcaac aaactgcaaa tcgtaaaata tgacgatgcc tgtgacccga aacaggcggt
    48001 tgcggtggcg aacaaagtcg ttaacgacgg cattaaatat gtgattggtc acctctgttc
    48061 ttcatcaacg cagcctgcgt ctgacatcta cgaagacgaa ggcattttaa tgatcacccc
    48121 agcggcaacc gcgccggagc tgaccgcccg tggctatcag ctgatcctgc gcaccaccgg
    48181 cctggactcc gaccaggggc cgacggcggc gaaatatatt cttgagaaag tgaaaccgca
    48241 gcgtattgct atcgttcacg acaaacagca atacggcgaa ggtctggcgc gagcggtgca
    48301 ggacggcctg aagaaaggca atgctaacgt ggtgttcttt gatggtatca ccgccgggga
    48361 aaaagatttc tcaacgctgg tggcgcgtct gaaaaaagag aatatcgact tcgtttacta
    48421 cggcggttat cacccggaaa tggggcaaat cctgcgtcag gcacgcgcgg cagggttgaa
    48481 aactcagttt atggggccgg aaggcgtggc taacgtctcg ctgtctaaca ttgcgggcga
    48541 atcagcggaa gggctgctgg tgaccaagcc gaagaactac gatcaggttc cggcgaacaa
    48601 acccattgtt gatgcgatca aagcgaaaaa acaggaccca agcggcgcat tcgtctggac
    48661 cacctacgcc gcgctgcaat ctttgcaggc gggcctgaat cagtctgacg atccggctga
    48721 gatcgccaaa tacctgaaag cgaactcggt tgataccgtg atgggtccgc tgacgtggga
    48781 tgagaaaggt gacctgaaag gctttgagtt cggtgtattt gactggcacg ccaacggcac
    48841 ggcgaccgat gcgaagtaat cattaatcgg caactttggg ttgccgccaa attgctaata
    48901 tcgagtacgt tgcttcatgc cggatgcggc gtaaacgcct tatccggcct acaagatcca
    48961 aagaaatcag taaattgcag cacacattgt aggcctgata agcgtagcgc atcaggcaat
    49021 ttctcgttgg tcatcacttt tgaaatcaaa cttgacgatt aacgcttctc ccagccgccc
    49081 tgctgtgccg taaatcccag tgcctgcata aacgccgtca tcacaccgcg atcttctacg
    49141 cctgcatccg ccatccacca ggatgaaacg ctggcattat cacttaacac ctcttccagc
    49201 agatattgcc ccacgccgcg acgtcgggtg acttcccgca cgcgcagaga atccagtgcc
    49261 cccccggtgc cgctcaaggt tacgcgtaca gcagcaagca ggcgctcgtt gaaacgtgcg
    49321 gcgtagatgc ggtggttatc gtcaacctgt aaagaggaag gggaatactc cggccagatc
    49381 ttttgcaggt caatccggtc ctggtcgcta aatttttcta atcgaatgat ggtcagcttc
    49441 atgggtaacc cgtgtaaatc acaaaagtgg aaccagtgta gcgaaataat ttaatcggag
    49501 gctttctctt ttttatttct tttggcaggt gattaatttt ttaacag
//
LOCUS       DAZUCE010000009        49040 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZUCE010000009 DAZUCE010000000
VERSION     DAZUCE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.009"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..202
                     /locus_tag="ACHY03_000488"
     CDS             <1..202
                     /locus_tag="ACHY03_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS3903767.1"
                     /translation="SAQDRAFIYEFARMIFAYINDKNIENLLLSCFAEVDQKAIQRYV
                     PQPQDAEKFWSNLQYKLSISAH"
     gene            413..736
                     /gene="ybjQ"
                     /locus_tag="ACHY03_000489"
     CDS             413..736
                     /gene="ybjQ"
                     /locus_tag="ACHY03_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFS3903768.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            733..1563
                     /gene="amiD"
                     /locus_tag="ACHY03_000490"
     CDS             733..1563
                     /gene="amiD"
                     /locus_tag="ACHY03_000490"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFS3903769.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMIPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1560..2573)
                     /gene="ybjS"
                     /locus_tag="ACHY03_000491"
     CDS             complement(1560..2573)
                     /gene="ybjS"
                     /locus_tag="ACHY03_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS3903770.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2672..4102)
                     /gene="ybjT"
                     /locus_tag="ACHY03_000492"
     CDS             complement(2672..4102)
                     /gene="ybjT"
                     /locus_tag="ACHY03_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFS3903771.1"
                     /translation="MPQRILVLGASGYIGQHLVQALSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGESGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4113..5114)
                     /gene="ltaE"
                     /locus_tag="ACHY03_000493"
     CDS             complement(4113..5114)
                     /gene="ltaE"
                     /locus_tag="ACHY03_000493"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFS3903772.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKDAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5151..6869)
                     /gene="poxB"
                     /locus_tag="ACHY03_000494"
     CDS             complement(5151..6869)
                     /gene="poxB"
                     /locus_tag="ACHY03_000494"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFS3903773.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKAAEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7002..7970)
                     /gene="hcr"
                     /locus_tag="ACHY03_000495"
     CDS             complement(7002..7970)
                     /gene="hcr"
                     /locus_tag="ACHY03_000495"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFS3903774.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLASVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVIAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(7982..9634)
                     /gene="hcp"
                     /locus_tag="ACHY03_000496"
                     /gene_synonym="priS"
     CDS             complement(7982..9634)
                     /gene="hcp"
                     /locus_tag="ACHY03_000496"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFS3903775.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREVLKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9778..10677)
                     /gene="lysO"
                     /locus_tag="ACHY03_000497"
     CDS             complement(9778..10677)
                     /gene="lysO"
                     /locus_tag="ACHY03_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFS3903776.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11124..11819)
                     /gene="aqpZ"
                     /locus_tag="ACHY03_000498"
     CDS             complement(11124..11819)
                     /gene="aqpZ"
                     /locus_tag="ACHY03_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFS3903777.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            12245..13903
                     /gene="ybjD"
                     /locus_tag="ACHY03_000499"
     CDS             12245..13903
                     /gene="ybjD"
                     /locus_tag="ACHY03_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFS3903778.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(13900..14856)
                     /gene="ybjX"
                     /locus_tag="ACHY03_000500"
     CDS             complement(13900..14856)
                     /gene="ybjX"
                     /locus_tag="ACHY03_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFS3903779.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            15007..16122
                     /gene="macA"
                     /locus_tag="ACHY03_000501"
     CDS             15007..16122
                     /gene="macA"
                     /locus_tag="ACHY03_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFS3903780.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            16119..18065
                     /gene="macB"
                     /locus_tag="ACHY03_000502"
     CDS             16119..18065
                     /gene="macB"
                     /locus_tag="ACHY03_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFS3903781.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(18138..18362)
                     /gene="cspD"
                     /locus_tag="ACHY03_000503"
     CDS             complement(18138..18362)
                     /gene="cspD"
                     /locus_tag="ACHY03_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFS3903782.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            18512..18586
                     /gene="yljB"
                     /locus_tag="ACHY03_000504"
     CDS             18512..18586
                     /gene="yljB"
                     /locus_tag="ACHY03_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFS3903783.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            18685..19005
                     /gene="clpS"
                     /locus_tag="ACHY03_000505"
     CDS             18685..19005
                     /gene="clpS"
                     /locus_tag="ACHY03_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFS3903784.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            19036..21312
                     /gene="clpA"
                     /locus_tag="ACHY03_000506"
     CDS             19036..21312
                     /gene="clpA"
                     /locus_tag="ACHY03_000506"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFS3903785.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(21716..21803)
                     /locus_tag="ACHY03_000507"
     tRNA            complement(21716..21803)
                     /locus_tag="ACHY03_000507"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21767..21769),aa:Ser,seq:gga)
     gene            complement(22057..22275)
                     /gene="infA"
                     /locus_tag="ACHY03_000508"
     CDS             complement(22057..22275)
                     /gene="infA"
                     /locus_tag="ACHY03_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFS3903786.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(22560..23264)
                     /gene="aat"
                     /locus_tag="ACHY03_000509"
     CDS             complement(22560..23264)
                     /gene="aat"
                     /locus_tag="ACHY03_000509"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFS3903787.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQIIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(23306..25027)
                     /gene="cydC"
                     /locus_tag="ACHY03_000510"
     CDS             complement(23306..25027)
                     /gene="cydC"
                     /locus_tag="ACHY03_000510"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFS3903788.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(25028..26794)
                     /gene="cydD"
                     /locus_tag="ACHY03_000511"
     CDS             complement(25028..26794)
                     /gene="cydD"
                     /locus_tag="ACHY03_000511"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFS3903789.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(26917..27882)
                     /gene="trxB"
                     /locus_tag="ACHY03_000512"
     CDS             complement(26917..27882)
                     /gene="trxB"
                     /locus_tag="ACHY03_000512"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFS3903790.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            28427..28921
                     /gene="lrp"
                     /locus_tag="ACHY03_000513"
     CDS             28427..28921
                     /gene="lrp"
                     /locus_tag="ACHY03_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFS3903791.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            29056..33162
                     /gene="ftsK"
                     /locus_tag="ACHY03_000514"
     CDS             29056..33162
                     /gene="ftsK"
                     /locus_tag="ACHY03_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFS3903792.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     DITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPEPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPV
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            33317..33928
                     /gene="lolA"
                     /locus_tag="ACHY03_000515"
     CDS             33317..33928
                     /gene="lolA"
                     /locus_tag="ACHY03_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFS3903793.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            33939..35282
                     /gene="rarA"
                     /locus_tag="ACHY03_000516"
                     /gene_synonym="mgsA"
     CDS             33939..35282
                     /gene="rarA"
                     /locus_tag="ACHY03_000516"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFS3903794.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            35373..36665
                     /gene="serS"
                     /locus_tag="ACHY03_000517"
     CDS             35373..36665
                     /gene="serS"
                     /locus_tag="ACHY03_000517"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFS3903795.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            36904..39348
                     /gene="dmsA"
                     /locus_tag="ACHY03_000518"
     CDS             36904..39348
                     /gene="dmsA"
                     /locus_tag="ACHY03_000518"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFS3903796.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            39359..39976
                     /gene="dmsB"
                     /locus_tag="ACHY03_000519"
     CDS             39359..39976
                     /gene="dmsB"
                     /locus_tag="ACHY03_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFS3903797.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRICEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            39978..40841
                     /gene="dmsC"
                     /locus_tag="ACHY03_000520"
     CDS             39978..40841
                     /gene="dmsC"
                     /locus_tag="ACHY03_000520"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFS3903798.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAETQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(40877..41503)
                     /gene="ycaC"
                     /locus_tag="ACHY03_000521"
     CDS             complement(40877..41503)
                     /gene="ycaC"
                     /locus_tag="ACHY03_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFS3903799.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            41818..42966
                     /gene="ycaD"
                     /locus_tag="ACHY03_000522"
     CDS             41818..42966
                     /gene="ycaD"
                     /locus_tag="ACHY03_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3903800.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            43176..44606
                     /gene="ycaM"
                     /locus_tag="ACHY03_000523"
     CDS             43176..44606
                     /gene="ycaM"
                     /locus_tag="ACHY03_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS3903801.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(44607..44717)
                     /locus_tag="ACHY03_000524"
                     /pseudo
     CDS             complement(44607..44717)
                     /locus_tag="ACHY03_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(44816..45556)
                     /gene="pflA"
                     /locus_tag="ACHY03_000525"
     CDS             complement(44816..45556)
                     /gene="pflA"
                     /locus_tag="ACHY03_000525"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFS3903802.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(45748..48030)
                     /gene="pflB"
                     /locus_tag="ACHY03_000526"
     CDS             complement(45748..48030)
                     /gene="pflB"
                     /locus_tag="ACHY03_000526"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS3903803.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(48085..48942)
                     /gene="focA"
                     /locus_tag="ACHY03_000527"
     CDS             complement(48085..48942)
                     /gene="focA"
                     /locus_tag="ACHY03_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFS3903804.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        12016 a        12265 c        13038 g        11721 t
ORIGIN      
        1 aagcgctcag gatcgggcgt ttatatacga gtttgcaaga atgatctttg catatattaa
       61 tgacaaaaac attgagaact tattgctttc ttgttttgcc gaggtcgatc aaaaagcaat
      121 ccagaggtat gttcctcagc cacaagatgc ggaaaaattt tggagtaatt tacagtataa
      181 gttgagtatt tccgctcatt aatgttgctg gtacaacata atacgacagg ttagcggtgg
      241 cctataatga aaccaatacc gggttatgca acggataatc gtaaagactt aagtgggtgt
      301 ttttgacaca atgagatgat ttcctaaact gatcaaaacc ttcttcccaa catggcatat
      361 cagtccgata atgctttaga gatcgaaggc ttaacaaaca aggagatcga tcatgcaatt
      421 ttccacaact ccaactctgg aaggccagac catcgttgaa tattgcggtg tggtgactgg
      481 cgaagcgatt ttaggcgcga atattttccg tgatttcttt gccggtattc gcgatatcgt
      541 tggcggacgc tccggtgcct acgaaaaaga gctacgtaaa gcgcgggaga tcgcctttga
      601 ggagcttggc tcccaggcgc gggcgctggg ggccgatgcc gtagtcggta ttgatatcga
      661 ctacgaaacg gttgggcaaa acggcagtat gctgatggtt agcgtcagcg gtacggcggt
      721 gaaaacgcgt cgatgagaag agtcttctgg ctggtcgctg ccgctctgtt actggcaggg
      781 tgtgcaggcg aaaaaggcat tgtcgagaaa gagggatacc agcttgatac ccgacgccag
      841 gcgcaggcgg cgtatccgcg cattaaagtg ctggtgatcc actacaccgc agatgatttt
      901 gatagctcac tggcgacact gaccgataag caggtcagtt cgcattatct ggtccctgcg
      961 gtgccaccgc gatacaacgg taaaccgcgc atctggcaat tggtgccgga acaagaactg
     1021 gcctggcatg cggggattag cgcctggcgc ggggcaacgc gccttaacga cacctctatt
     1081 ggcattgagc tggaaaaccg tggctggcaa aaatcggcgg gggtgaaata ttttgccccg
     1141 tttgaaccgg cacagattca ggcgcttatt ccgctggcga aagatattat tgcccgttat
     1201 cacatcaagc cggaaaacgt agtggcacat gcggatatcg ccccgcagcg caaagacgat
     1261 ccggggccat tatttccctg gcagcaactg gcgcagcagg ggattggtgc ctggccggat
     1321 gcgcagcggg tgaactttta ccttgccggg cgcgcgccgc acacaccggt agataccgcg
     1381 tcattgctgg agcttttggc gcgctacggt tatgacgtta aacctgatat gataccgcgc
     1441 gagcagcggc gcgtgattat ggcgttccag atgcatttcc gcccgacgtt atataacggc
     1501 gaagcggatg cagaaactca ggcgattgcc gaagcgttgc tggagaaata cgggcaggat
     1561 tagcgcggca gttttccgtg gtcgcgcagc caggcggcag ttttctcgat accttcatcc
     1621 agggtgatga ccggctgata acctaactct tcctgtgccc gcgtaatatc cagcgtaaag
     1681 tcaaaattaa gcttcgagac gccgtagtgg gtcagcggcg gctcttttgc tgacttgcgg
     1741 cctaaacgct ccatgctgcg ggcgatcata tccagcatcg ggtaagggac ggaacgaata
     1801 cgacagtcaa tattcaactc gtcgatcagc ttctgcacga tgctgcgcag tgtgcgatgc
     1861 tcgccgttgg tgatgttgta cacacgcccg gaaggtagct tatcgcaggc ttcctggctt
     1921 gccagccaca tggcgtgcac ggcattttca tagtaggtca tatccaccag cgcactgccg
     1981 ccatgcggta acagaatgct gccgtagtgg tgcatcatat gcgcaagacg ggggataaag
     2041 actttatcgt gcggtccgaa cagactttgt gggcgcagaa tagtaaagcg cgtttgtgga
     2101 ttcgcctgcg aaagcatatt gatcacttct tcgctggctg ctttgctgcg ggcaaactcg
     2161 ttggcgaagc ggtgagggcg aaaatcttct ttaatatcgc gatggtggtg ataatcgaag
     2221 tacagggagg gggaagagat atgaataaag ttacgcacac cccaggcgac agcccattca
     2281 cccaggcggc gagtggcgcg aacgttagcc agatcgaaag cctgttgtgt cccccagggc
     2341 gaggtaaagc tggagcagtg ccacagcgta tcaatgcccg cgagcatcac tttagcttgt
     2401 gatgagacca gctcggtcag atccgccgga acaaactctg cgcccatttt ttccagcaat
     2461 ttacccattg cctcgttgcg accggtcgct cgcacgctga tgcctttctg gcataaaaac
     2521 tctaccgcgt ttcgacctaa gccgctggtg gcgccggtaa ccagtacctt catatcaatc
     2581 cactgttgtt gagaaaatga cgtgcgcatt cttccgtgat ttcccccttg atgcaatggg
     2641 aaacatgaaa gattaacgca ggttttatcg attaatctgt gctttgttct gccagtctgg
     2701 cgatttgttt tgccattccg cgaaaaataa acagatgcgc ggggatcatc aataaccagt
     2761 aaaacagccc cggcataccg tgcggatgcc agaaagcgcg gacatcgata gtacgatagt
     2821 cgcctttatc ttccagggta aaacacagtc gtcccagtcc cggcgctttc atgccaaata
     2881 acaacgtaag ttgtttttcc ggttcaacga caatcacttt ccagctatcc accgcatcgc
     2941 cggtctgtaa atattcgcgc tccgggcggc ctttcgccag cttatgaccg atcgcgcggt
     3001 ccatcaacgc ccgtgtctgc cacaaaatat tgccaaagaa ataacgctct ttaccgccga
     3061 tttggttcac tacctgccat aaagccgcaa ggctggcgga cgttttaacg gtaaaccccg
     3121 cctgtttggc gaaataaccg tactcaggtc gccagcgggc aaaggcctga gcgtcgtagc
     3181 cccagtcgct ggagttgacc agtttttctt cctctttcaa cgtgctacgt accgcgtcat
     3241 cgaaagcgat cagccgttgt gggatgagcg cacgtagcgc ggtgtcatcc gccagcagat
     3301 cgtgtttcag cccctgaatc aacgccctgg cggtggtggg cggtacggaa gtaatcacat
     3361 tgagaaacca caccgaaatc cagcgggtgg ggagggggat ggggatcaac cagcggcgct
     3421 taccgctcac cgccataaaa tgttcaaact gttgctgata actgagcacc tctggtccgg
     3481 cggcttcgaa gatgcggtgt tcgctggccg gatgatctaa cagcgccacc agatagtgca
     3541 gcaagttttc cagcgcgata ggcgtggtgc gtgagcgtac ccagcgtggc ggcgttaaca
     3601 ctggcaggtt gtaaaccata tcgcgcatca cttcgaacgc tgcggaacct gcgccaacga
     3661 taattccggc ccgaagttcg gtcactggta cattcgcttc acgaagaatg tccgccgtag
     3721 cctgacgagc acgcagatga tccgactgct catgtggcgg ggcctgcaac gaactgagaa
     3781 agattaattg cttaactggt acttcacgta gcgcatcgcg gacgttgaga gccacctggc
     3841 gctcctgagc gataaaatcg ccgctttcgc ccatgctgtg caccagaaaa tagacggtat
     3901 cgatatcctg taacagggcc ggaaggttat ccggccagct gagatcgact ttatggcaac
     3961 tgacgtttgc cagttgcagc tttgcaagcc tgtcgacatg acgtgccgcc gccaggatct
     4021 gatgcccttg ctggctgagt gcctgcacca gatgttgacc aatgtagcca ctggcaccaa
     4081 gaactaaaat gcgttgcggc acgtctccct ccttaacgcg ccaggaatgc gcgccagtgg
     4141 gcggcgactt ctgccagttg ttcgcgcgag acgtcaagat gggtcaccag gcggacaatc
     4201 ggcgaggcgt taatcagtac gtttctcgct ttcatgtatt cgcctaacgc ggcagcattt
     4261 tcttcgccga cgcggacaaa cagcatatta gtgtcctgac gcattacatc cgcgcccgct
     4321 tcgcgcagtt gctccgccat ccaggcggcg ttgtcgtgat cttcctgcaa gcgtgcgacg
     4381 ttatttttca gcgcatacat cccggcagca gccagaatgc cggactggcg catcccgcca
     4441 cctgtcattt tccgccagcg aatggcacgt ttaatgtaat cacgattacc gacgagtaat
     4501 gaaccgactg gcgtcccaag acctttcgac aggcaaatgg tgaacgaatc acaatattgc
     4561 gtgatctctt tcagttcgca gccgtaagcc accacggcat taaagatgcg cgcaccgtca
     4621 acatgcagcg ccagattgcg ctcgcgggta aattcccatg catctttaag gtattcacgc
     4681 ggcaacactt tgccgttgtg ggtgttttcc agactgagta atttggtgcg ggcgaaatgg
     4741 atatcgtcgg gtttgatttt catcgccact ttatccagcg gtagcgtgcc gtcggcagcc
     4801 gcgtcgatgg gttgcggctg aatactgccc agcaccgctg cgccaccggc ttcaaacaga
     4861 tagttatgcg cggcctgacc gacaatatac tcttcgccac gttcgcagtg actgagcaga
     4921 gcgaccaggt tggcctgagt gccggtaggc agaaaaatgg cggcttcttt accggaaagc
     4981 tctgctgcat agtcctgcag agcattaacg gtagggtcgt ctccgtaaac gtcgtcccca
     5041 accggggcgg ccatcatcgc ttcgagcatg gcgcggctcg gtcgggttac ggtatcactg
     5101 cgtaaatcaa tcatggcatg tccttattat gacgggaaat gccacccttt ttaccttagc
     5161 cagttcgttt tcgccagttc gatcacttca tcgccgcgtc cgctgatgat tgcgcgcagc
     5221 atatacaggc taaaaccttt ggcctgttcg agtttgatct gcggtggaat ggctaattct
     5281 tctttggcga ccaccacatc caccagcacc ggaccgtcga tggaaaaggc gcgttgcagg
     5341 gcttcatcga cttcagcggc tttttctaca cggatacccg taatgccgca cgcttcggca
     5401 atgcgggcaa agttggtgtc gtgaagctcg gtgccgtctg tcaggtagcc tccggctttc
     5461 atctccatcg ccacaaagcc cagcacgctg ttgttaaaga cgataatttt caccggcagt
     5521 ttcatctgta ctactgagag gaaatcgccc atcaacatgc taaaaccgcc atcgccgcac
     5581 atggcgacca cctgacgttc cggctcggtt gcctgcgcac ccagcgcctg cggcatggcg
     5641 ttagccatcg aaccgtggtt aaacgaacct aacaggcgac gcttgccgtt cattttcaga
     5701 taacgcgctg cccatactgt tggcgtgccg acatcacagg taaagatggc gtcatcggcg
     5761 gcaaaatgac taatttgctg cgccagatat tgcgggtgaa tggctttctc gctcggttta
     5821 gctaaatcgt ccagcccttt gcgggcatcg cggtaatctt ccagcgcttt atccagaaac
     5881 ttgcgatcgg ctttttcttc caccaatggc agcaatgcac gcagggtcga cttgatatcg
     5941 ccgaccagcg ccatatccac cttgctgtgt gcgccgatgc tcgctgggtt gatatcaatc
     6001 tgaataattt tggcatcggt cgggtagaaa gcacggtagg ggaactgggt gccgagcagc
     6061 actaaggtgt cggcgttcat catggtatgg aaacctgacg agaagccgat taacccggtc
     6121 attccaacat catacggatt atcgtattcg acatgttctt taccgcgcag ggcatgaaca
     6181 ataggtgctt taattttccc ggcaaactca actaactctt tatgcgcccc cgcgcagccg
     6241 ctgccacaca tcagggcgat attgctggaa taacgcagca gttgcgccag tttgcgtaac
     6301 tcttcttctt ccggcgtcac gaccggctgt ggcgcatgat accagtgggt agttgcccct
     6361 tctggcgcag gttttaacgc cacgtcgccc ggtaacacga caacggaaac gccacggtta
     6421 agcaccgctt tgcgcatggc aatcgccagc acttgtggga tctgctccgg gctggaaacc
     6481 agctcgcaat agtgactaca ttcgcggaat agctcttgtg ggtgggtttc ctggaaatag
     6541 ccgctgccta tttcgctgga gggaatatga gcggcaatcg ccagtaccgg aacgtgattg
     6601 cggtggcaat cgaacaggcc gttgatcaag tgcaggttgc ctgggccgca cgatccggca
     6661 cagaccgcca gttctcctga aagttgtgct tcagcgccag cggcaaacgc cgccacttct
     6721 tcatggcggg tggacatcca ctcgatggtg cccatgcgat taagactgtc gctaagaccg
     6781 ttcagagagt cgcccgtgac tccccagatg cgtttcacgc ctgccgattc gagtgttttg
     6841 gcgatataag ctgcaaccgt ttgtttcatg gttctccatc tcctgaatgt gataacggta
     6901 acaagtttag ttcatctgac ggagggggaa gggatgggag agaaaggagg cactaacggt
     6961 taaatagccc gatgaaagga atatcatcgg gcataaggcg attatgcgag aaccaaatcc
     7021 ccctgcggat ggcaggagca ggccagtacg taaccttcag cgatttcggc gtcggtcagc
     7081 gtcattgtac tgctcaccgt atattcaccg gaaaccactt tcgtcttaca gcagccgcaa
     7141 acacccgcgc ggcaggcggc gataaccgga acgttattgc tttccagcgc ctccagtagc
     7201 gtggtgccaa ccggggcgta aaattctcgt gccggttgca gtttggtgaa tttcagaccg
     7261 ctggtcgccg cttccgctac tggggtgaag aatttctctt taaagaaacg cgtcacgccg
     7321 agcgccttca cttcctgttc tacccaatcc atatacggtg ctggaccaca agtcatcacg
     7381 gtacgtgagg ctaaatcagg aacgcttgcc agcagttcgc gagtgagacg accagcgata
     7441 aagccttcgg taacgttatt ttccgccacc agcgttaccg gatagttacg ccactcatcg
     7501 gcgaaaataa catcctgcgg cgtacgcacg ttgtagatca cccgcacatc ggcctgtgga
     7561 cggttcttcg caagccagcg acgcatcgac ataatcggcg tgacgccgca gcctgccgcc
     7621 agcaacagga atttatcttc tgctttatcg tcgcaggtaa attcccccat cgcgtccgaa
     7681 agccagagat aatcaccgcg ttttacatcg cgcgtcagcc actgggagcc tacaccgtca
     7741 tcaatccgcc gcacggtcag ggtgatatat tcactcactc ctggcgtgga ggaaatggtg
     7801 taagcacgca gcgtttctgc tgagtttcgc acgctgacca gtgcatattg cccggcgcga
     7861 tatgggtagt aatcgtggca aatcagggaa atcgtccaca catccggcgt ttcttgctta
     7921 atgtgatgaa cctgcatccg ccacgggcat tgattcgttg gcatcgtcat ctgctaactc
     7981 cttacgcgct caacagttgc ttcatgtctt cttcaacagt ggtgatagaa cgcaggccga
     8041 atttctcgtt cagcaccgcc agcagatctg gtgtcaggaa accaggtgca gtcgggccgg
     8101 tgacgatatt tttcacgccc agagaaagca gcgtcagcag aatgacaatc gctttctgtt
     8161 caaaccagga gagcaccagc gacagcggca gatcgttgac accgcagccc agtttttctg
     8221 ccagcgtaac cgccagaata attgctgagt aagcatcgtt acactgaccg gcatccacca
     8281 gacgaggcag accttcgata tcgccaaact caagtttgtt aaagcgatat ttaccacagg
     8341 cgagagtcag gatcaggcag tcatccggca cgctggtggc gaaatcggtg aagtagtgac
     8401 gttcgccgcg tgcgccgtca cagccaccaa gcaggaagat atgacgcagt ttttcacggc
     8461 tcaccagatc aatcagcgta tccgctgcgc caagcagcgt ctggcggcca aagcccacgg
     8521 tgatcaggtg cggaatttcg ctgtacggga agcccgccat ttgttgcgcc tgggcgatga
     8581 ccgcagagaa atcttcgcca tccagatgac gcacgccagg ccagccaacg atgctgcggg
     8641 tccagatacg atcgtcataa gcgcctacgg ttgggtcgat gatgcagttc gaggtcatca
     8701 cgatggggcc agggaaacga gcgaactcca cttgctgatt ctgccagccg ctgccgtagt
     8761 taccgaccag atgcttgaat ttacgcagct ccgggtagcc atgcgcaggc agcatttcgc
     8821 cgtgggtgta gacattaacg cccgtgcctt cggtctgttc cagcaggttg tagagatctt
     8881 tgagatcgtg accggaaatc aggatgcatt tacccgccgt cgctttgacg ttgacctggg
     8941 ttggcgtcgg gtgaccgtat ttaccggttt cgcctgcatc cagaatgctc atcactttga
     9001 agttcatctg gccgatttcc attgaacact caagaagcgc gttcatatcg gcaggccagg
     9061 tccccagcca cgccatgatt ttatgatact gggcataaat atcgttgtcg tattgaccga
     9121 gaacgtgtgc gtgttccata taggccgccg cacctttcag gccatacagg cacagcagac
     9181 gcaggccgag aatgttttcg ccaatcgccg ctttatcttt gttaggggta aattctgctg
     9241 cctgacgttg cagctcgccg agatcgtcgc tcaccagttg caggtcagcc atcgggttat
     9301 cgacgcgcgc gttggcatct acagccaggc attgtgcttt cagcacctcg cgcagggcaa
     9361 tcgcttcacg agcgtagccg actatacgcg gagaatcgaa gttaacgttg gtcagggttg
     9421 agaaaaatgc gcgtggcgca aagctgtcta catcgtggtt gatgatgccg tattcacgcg
     9481 cttttaccgc ccaggccgaa agcccttgca gcgccgcgat gagtaaatcc tgaaggtcag
     9541 aggtttccgc cgttttacca cacatcccct gcgcgtatga gcagccgttt cctgccggag
     9601 tacggatagt ttgttcacat tgcacacaaa acatgatcac accttttaaa gttatattta
     9661 atatacatgt ttaaggttaa gacgcttaac gcggggataa aagggatttt tcatgcaact
     9721 ttaagggaga ttgatttagc gcaattttgg cggcagggat ctaccgccag agaggtatta
     9781 cgcagagaaa aaggcgatga ggatcggcac taacaggcta agaataaaac cgtgaacaat
     9841 tgccgccggg accatatcca gcccgccagt acgttgaaga acgggcaggg tgaaatccat
     9901 cgacgtcgca ccgcataagc ccagtgcggt agagcggctg cggcgaatca gcccagggat
     9961 caacataatt gcaatcagct cacgggccag atcattaaaa aacgccgcgc tcccgattac
    10021 cggaccaaaa gattcggtca ataaaatacc ggaaagagaa taccagccga aaccagaggc
    10081 cattgccagc gcggtattga tgggtagatc aagaataaag gcgttaatta aaccaccaat
    10141 taatgaactg gcaaccacca ccacggcgac aatcattccc cggcgattaa ggacaatctg
    10201 ctttaaggtc atgccattat tgcgcaactg aataccgacg aggaaaagta gcagaattaa
    10261 cgtgtattca ctggcttcgg tcgcgtgttg taagaaagcc agtccactca ggccaatggc
    10321 aaaaccaatc actactacgc cgcacagttt tagcgactcc agcgccatcg caatacgcga
    10381 cgggagtttt tcttgctgat ggtggttgcg ccacggcaga ccgcgctcca gccacatcag
    10441 ggcggcaata ttacacagta aaataacggt aatactgacg gcagaataat gcagaatcgc
    10501 caacaggtta ctggcgaggt tatcgagaaa cgccagactg atacccataa aaaagagaat
    10561 aaggtaaacc atccagctta agagctgatt aataactttt aatgcagctt tttggcgaag
    10621 cggaatgagg taacccacaa tcaggggaac cagaatgatt aacagcccag aaaacatgaa
    10681 aaaccagtcc ttgcaaagaa agaagtcgaa atgcgcgatg acacactact gaaagcggaa
    10741 ggacgagtaa agttgcaatt aaaaggaaat gttatgcatc agagggggat ttttaattct
    10801 atatgagtgt ttagcattgt tgcgctcctt tcatgtgcaa tgccatataa agccactggc
    10861 gcagggagcg gccagtcgcc gccgccccct gcacccccgg gctctggcga acaaaatcgc
    10921 cgctgcgcgg taccttcagc ttatcccctt cggcaaccgg gtcgggcgcg aggtaacgtc
    10981 ctgtaagacg cgccctcagc ccacatccat gtgggctgcc ccggccttcg gggaacgctt
    11041 cagcgatttt gacgccacca atacccgtgc ggtttgtaat gtcgaagata aaacaaccag
    11101 ggcagataaa atgccaatgt taatcaatcg cgtttttcca gcagagtccg gtaaatcaga
    11161 ccaccgataa tgccaccgac aattggcacc acccagaaga accacagttg ttctaatgcc
    11221 cagccgccct ggaagatggc aaccgcggtg ctgcgcgccg ggttaacaga agtgttagtc
    11281 accggaatac tgattaagtg aatcagggtt aaggccagac caatagcgat cggcgcaaaa
    11341 cctgccggag cgaatttgtc ggttgcgccg tggatcacca acaggaaacc tgcactcaat
    11401 accagttcaa ctaccagcgc ggaaagcatg gaataaccgc ctggtgaatg ctcgccataa
    11461 ccgttagaag caaaaccgct ggctgccgcg tcaaaacccg ttttaccact ggcaattaaa
    11521 tacagcagcg ccgctgcaac aataccgccg acaacctggg caattacgta gccaacgact
    11581 tcttttgccg gaaaacgtcc gccagcccat aaaccaatag tgaccgccgg gttaaaatga
    11641 ccaccagaaa tatgaccaac agcaaaggcc atcgtcagaa cggtcagacc gaacgccaac
    11701 gccacgccgg caaaaccaat gcctaattcc gggaagcctg cggccagcac agcactacca
    11761 cagccaccaa aaacaagcca gaaagtacca aaacattcag ctgctaattt tctgaacata
    11821 tccacctcaa ttaaaaattg accctgtgaa aaatatggtc gttttatagg gtcgtcgtaa
    11881 aaaagtgacg acggaaataa tgcgcggcta ttttaaaaac gaaggcgagt cattcaccag
    11941 ataaataaat ccagtaaatt tgatttaggg caacagcggg ttgccccata tagtcatttg
    12001 tctgattgac agtgtagtgc aagcaaaaga tttaatcctt taggcgtaat aaaaaataat
    12061 ttatcatgct aattatttga ttttgttgtt tttgcagact tatcagcaag agggagtata
    12121 acgcgattat tcgctcattt ttcagacatt tgccatgctt aaatgtgatg tcatcacgta
    12181 ttagcaaggc ctttcccgtt atactgccag cgtaaaggat aagtcacata tttctggagg
    12241 ggatatgatt cttgagcgcg ttgaaattgt gggttttcgc ggtatcaacc gtttgtcgtt
    12301 gatgctggaa caaaacaacg tcctgattgg ggagaacgcg tggggtaaat ccagcttgct
    12361 ggacgcctta accctgctgc tatcgccaga atcagatctc taccattttg agcgcgacga
    12421 tttctggttc ccgccgggag atatcaacgg gcgagaacat catctgcata ttattttgac
    12481 cttccgcgaa tcgctgccag gccgacatcg ggttcgccgt tatcgaccgc tggaagcgtg
    12541 ctggacgcca tgcaccgatg gctatcaccg tattttttat cgtctggaag gggagagtgc
    12601 ggaagacggc agcgtgatga cactgcgcag ttttctcgat aaagacggac atccgattga
    12661 tgttgaggat attaacgatc aggcacgcca cctggtgcgt ttaatgccgg tgctgcgctt
    12721 gcgtgatgcc cgttttatgc gccgtattcg taacggcacg gtgccaaatg tccccaatgt
    12781 ggaagtcacc gcgcgccagc tcgatttcct cgcccgtgag ttgtcctcac atccgcaaaa
    12841 tctctctgat ggacagattc gtcagggact ttccgcaatg gtgcagttgc ttgagcatta
    12901 tttctctgag cagggggctg gacaggcgcg atatcgttta atgcggcggc gggccagcaa
    12961 tgagcaacga agctggcgct atctggacat catcaaccgg atgattgacc gacctggagg
    13021 gcgctcgtat cgggttattt tgctcggcct gtttgctact ttattgcagg caaaaggcac
    13081 attgcgactg gataaagacg cccgtccatt gttgctgatc gaagatccag aaacgcgttt
    13141 acaccccatt atgctttcag ttgcctggca tctgttgaat cttttgcctt tacaacgtat
    13201 tgctaccacc aactctggcg agctgctttc gttaactcca gtggaacatg tttgccgcct
    13261 ggtgcgtgaa tcttctcgtg ttgccgcctg gcgtctgggg ccaagtggct tgagcaccga
    13321 agacagccgc cgcatctctt ttcacattcg ttttaatcgt ccgtcatcgc tgtttgctcg
    13381 ctgctggttg ctggtggaag gggaaacgga aacctgggtt atcaatgaac tggcgcgtca
    13441 gtgcggacat cattttgatg ccgaaggaat caaggtcatt gagtttgccc agtccgggct
    13501 aaagccactg gttaaatttg cccgccgaat ggggattgaa tggcatgtac tggtcgatgg
    13561 cgatgaagca ggaaagaaat atgccgctac ggtacgcagc ctgttgaata atgatcggga
    13621 agccgaacga gaacatttaa cggcgttacc ggcgctggat atggaacatt ttatgtatcg
    13681 ccagggattt tccgatgtgt tccaccgcgt ggcgcaaatc ccggaaaatg taccgatgaa
    13741 tctgcgcaaa attatctcga aagcgatcca tcgctcttcc aaacccgatc ttgccattga
    13801 agtggcaatg gaggccggac gtcgtggtgt ggactccgta ccgacgctgc tgaaaaaaat
    13861 gttctcacgc gtgctgtggc tggcgcgcgg tcgcgcggat taaccgcgaa acatcgtggc
    13921 catttgtggc tgaatagcgt cgagcatctc atagcgccga cggtattcag cccgtttttt
    13981 actggcgatt tcggcaatct cttttcgtgc tatctgtgct ggaaggcggt aatggcgttc
    14041 agcatcacat acgccgccaa ccgattccca gaaagcgttg taatcggcat ggatcttgcc
    14101 ttctttatcg cgataacgca ggctgcggta aatatgcgtt tcattgctga cggcaataat
    14161 ctgctctacc tgcaaacgtt gggcaaacag acaggccgct tccatcacga ggcgtttggg
    14221 aaatagcccg tggcaggctt tcgtcgcatt ctggatttcc tgatgtggaa tttcccattt
    14281 tgcgccttgc agtccaccaa taaacatcgt tcttttcccc tgatattcac acagggtaaa
    14341 cgtgatttct gccagaggaa taccttcgct gttgcggaac aggattgtgc tgtcaccttc
    14401 tttatccatt gagatcatca tggtcagctc aagcgtgaac tgctcgccgt ttttgccttc
    14461 cagcttcgcc agttgcagcc cgggggtatt caaatataag ctgaattctt ccgccgacat
    14521 acacccgcgg agtaacgcat aatggtaacg taacgcctcc agcaattgct tacggctaag
    14581 attcgccgca aggtaagggc gatgcagacg tacaggcagt cgcggctggc gcgttaacaa
    14641 cacattgaga ttaggccagt gggaaagttc gttcatccac tcaacgctta aacgcggcat
    14701 aatcaacgag cgcagcaaaa atttctggcg aaaactacgg cgatgccaga atttacccgg
    14761 ccgacactgt ccacgtgcca gactaagaaa aagtgacagg ctgctgagag attcagatga
    14821 cgtaaaggtc cgttcggtta gctgcgacat attcatgaaa tcaatggttg tacatgacgt
    14881 cgatttcacc attgcgtatc ttaaccaaac atcaatagtg tgattactaa cgtaaatttt
    14941 agggttttgt tgatatttcg ttgaagttaa tgacccggat tggcatatgg agtattcaga
    15001 atatttatga aaaagcggaa aaccgtgaag aagcgttacg ttattgcgct ggtgatagtc
    15061 atcgccggac tgattacgtt atggagaatt cttaacgcac ccgtgccgac ttatcagaca
    15121 ctgattgtgc gccccggtga tttacagcaa agcgtgctgg cgaccggaaa gctggacgcg
    15181 ctgcgtaagg ttgacgtggg cgcgcaggtc agcggtcagt tgaaaacgct gtcggtggcg
    15241 attggcgata aagtaaaaaa agaccagctt ttaggggtta ttgatcctga acaggctgaa
    15301 aaccagatca aggaggtcga agcaacgctg atggagctac gcgcgcagcg gcagcaggcg
    15361 gaagcggagc tgaaactggc gcgggtgacg tattcccgtc agcaacgtct ggcacaaacg
    15421 caggcggttt cacagcagga tctcgacacc gccgcgacgg agatggctgt gaaacaggcg
    15481 caaattggca ccattgacgc gcaaatcaag cgcaatcagg cttctctcga tacggctaaa
    15541 accaatctcg attacactcg catcgttgcc ccgatggccg gggaagtcac gcaaatcacc
    15601 actctgcaag gccagacggt gattgccgca caacaagcac cgaacattct gacgctggca
    15661 gatatgagca ccatgctggt aaaagcgcag gtttctgaag cggatgtaat ccacctgaag
    15721 ccggggcaaa aagcctggtt tacggtactt ggcgatccac tgacgcgcta cgaggggcaa
    15781 atcaaggatg tactaccgac gccggaaaag gttaacgacg ctattttcta ttacgcccgt
    15841 tttgaagtcc ccaaccccaa tggtttgctg cggctggata tgactgcgca agtgcatatt
    15901 cagctcaccg atgtgaaaaa tgtgctgacg atccctctgt cggcgttagg cgatccggtt
    15961 ggcgataatc gttataaagt caaattgttg cgtaatggtg aaacacgcga gcgtgaagtg
    16021 acgattggcg cacgtaacga taccgatgtt gagattgtca aagggctgga agcgggcgat
    16081 gaagtggtga ttggtgaggc caaaccagga gctgcacaat gacgcctttg ctcgaattaa
    16141 aggatattcg tcgcagctat cctgccggtg atgagcaggt tgaggtgctg aagggcatca
    16201 ccctcgatat ttatgccggt gagatggtag cgattgttgg cgcttcgggt tccggtaaat
    16261 cgaccctgat gaatattctc ggctgtctgg ataaggccac cagcggcacc tatcgcgtcg
    16321 ccggtcagga tgttgccaca ctggacgccg atgcgctggc gcaactgcgc cgcgagcatt
    16381 tcggctttat tttccagcgt taccatttgc tttcgcattt aaccgccgag cagaacgttg
    16441 aagtacccgc cgtctatgct ggtcttgagc ggaaacagcg gctgcttcgc gcccaggagt
    16501 tgctgcaacg gctggggctg gaagaccgta cagagtatta tccggcacag ctttcgggcg
    16561 gtcagcaaca gcgcgtcagc atcgcgcggg cattgatgaa cggcggtcag gtaattcttg
    16621 ccgatgaacc aaccggcgca ctggacagcc attctggcga agaggtgatg gcgatcctgc
    16681 atcagctgcg cgatcgcgga catacggtga ttatcgtcac ccacgatccg caggtcgctg
    16741 ctcaggccga gcgggtgatc gaaattcgcg acggcgaaat tgtgcgcaat cctcccgcca
    16801 ttgaaaaagt gaatgttgct ggcgggacgg aacctgttgt caacacggtg tctggctggc
    16861 ggcagtttgt cagcggtttt aacgaggcgc tgacgatggc atggcgggcg ctggcagcga
    16921 ataaaatgcg tactttactg accatgctgg ggattataat cggtattgcg tcggtggttt
    16981 ccattgtcgt ggtgggcgac gccgccaaac aaatggtgtt ggcggatatt cgttctattg
    17041 gtacgaatac tattgatgtc tatcccggga aagattttgg cgatgacgat ccgcaatatc
    17101 agcaggcgct gaagtacgat gacttaatcg ccatccaaaa acaaccgtgg gtcgcttcag
    17161 ccacacctgc cgtctcgcaa aacctgcgcc tgcgttacaa caatgttgat gttgctgcca
    17221 gtgccaatgg cgtgagcggc gattatttta atgtctatgg catgaccttc agtgaaggaa
    17281 acacctttaa tcaggaacag ctgaacggtc gtgcgcaggt cgtggttctc gacagtaata
    17341 ctcgccgcca gcttttcccc cataaagcag atgtggttgg cgaggtgatt ctggtcggca
    17401 atatgcccgc cagagtcatt ggtgtggcgg aagaaaaaca gtcgatgttt ggtagcagta
    17461 aagtgctgcg tgtctggcta ccttacagca cgatgtccgg gcgagttatg ggccagtcgt
    17521 ggctgaactc cattactgtc agggtgaaag aaggatttga cagcgccgag gcggaacagc
    17581 aactcacgcg tttactttca ctgcgccacg gtaagaagga tttctttacc tggaacatgg
    17641 acggcgtctt gaaaaccgtt gaaaagacca cacgtacttt acaactgttt ctgacgctgg
    17701 tggcggtgat ttcgctggtg gtgggcggta ttggtgtaat gaatattatg ctggtgtcag
    17761 tgaccgagcg gacgcgggaa attggcattc gcatggctgt aggtgcgcga gcaagcgatg
    17821 ttttgcaaca gttcctgatc gaagccgtac tggtttgcct ggtcggtggc gcgttgggaa
    17881 taacactgtc actgttaatt gctttcacct tgcagctttt cttacccggc tgggagattg
    17941 gtttttcacc gttggcgctg ctgctggcgt ttctctgctc gacggtcacc gggattttat
    18001 ttggctggtt acccgcacga aatgcggcac gactggatcc agtagatgct ctggcacgag
    18061 agtaattttt gagataaaaa tgccagccga tcgggctggc attttgcctt taggatgtac
    18121 acaatgagac agaagagcta tgcgactgcc gcttctactt cgacgggcac aataacactg
    18181 gcgtgattgc cttttggccc ctggtggaca tcaaactgaa cggattgtcc agcttttagc
    18241 gttctgtaac catccatctg aatggtggaa taatgagcga aaatatcttc gccgccgcct
    18301 tcagggcaga tgaaaccaaa ccctttggca ttgttgaacc acttaacagt acccttttcc
    18361 atgcttcgac atccttcgca aatcttatac aagtaagatg gaataaaccg gggtcagaga
    18421 gggggctgtt caaaacctcg ccaactctag aaatacaatt tagagaatta gggcgagccg
    18481 tcaagcattt gacaggggcc agggggcagg tatgaatcaa aaatttgaag cagttaacgc
    18541 tattgacagg aatgtgacag atgtcgctga tgccaacgat agatgatagt tatctatcat
    18601 gtggagtaga ttggtcaggc aaataagctc ttgtcagcgg cagggcgttc tgccgataac
    18661 cgtaaccgaa gatgataact gacaatgggt aaaacgaacg actggctgga ctttgatcaa
    18721 ctggcggaag aaaaagttcg cgacgcgcta aaaccgccat ctatgtataa agtgatatta
    18781 gtcaatgatg attacactcc gatggagttt gttattgacg tgttacaaaa attcttttct
    18841 tatgatgtag aacgtgcaac gcaattgatg ctcgctgttc actaccaggg gaaggccatt
    18901 tgcggagtct ttaccgccga ggttgcagaa accaaagtgg cgatggtgaa caagtacgcg
    18961 agggagaatg agcatccatt gctgtgtacg ctagaaaaag cctgaatgca ggcataaaaa
    19021 ttgggggagg tgcctatgct caatcaagaa ctggaactca gtttaaatat ggctttcgcc
    19081 agagcgcgcg agcaccgtca tgagtttatg accgtcgagc acttgttact ggcgctgctc
    19141 agtaacccat ctgcccggga ggcgctggaa gcgtgttctg tcgatttggt tgcgctccgt
    19201 caggaactgg aagcctttat tgaacaaacc acacccgttc tgcctgccag tgaagaggag
    19261 cgcgacacac agccgacgct gagttttcag cgtgtactgc aacgtgcggt cttccatgtc
    19321 cagtcctccg gtcgcaatga ggttaccggt gcaaacgttc tggtcgctat ctttagcgaa
    19381 caggagtcgc aggcggcata tctgttgcgt aaacatgaag tcagccgtct cgatgtggtg
    19441 aactttatct ctcatggcac gcgtaaagac gagccgacac agtcttctga tcctggcagc
    19501 cagccaaaca gcgaagaaca agctggtggg gaggaacgta tggagaattt cacgacgaac
    19561 ctgaatcagc ttgcgcgcgt gggcggaatc gacccactga ttggtcgtga gaaggagctg
    19621 gagcgtgcta ttcaggttct ctgccgtcgc cgtaaaaaca acccgctgct agtgggggaa
    19681 tctggtgtcg gtaaaaccgc gattgcagaa ggtcttgcct ggcgaattgt tcagggcgat
    19741 gtgccggaag tgatggctga ctgtacgatt tactctctcg atatcggttc tctgttagcg
    19801 ggcacaaaat atcgcggcga ctttgaaaaa cgttttaaag cgttgctcaa gcagctggag
    19861 caggacacta acagcatcct gtttattgat gagatccaca ccattatcgg tgcgggtgca
    19921 gcgtctggtg gtcaggtcga tgcggctaac ctaatcaaac cgttgctctc cagcggtaaa
    19981 attcgcgtaa ttggttcgac aacctatcag gagttcagca acattttcga gaaagaccgt
    20041 gctctggcgc gtcgcttcca gaaaattgat attactgaac cgtcgatcga agaaactgtt
    20101 caaatcatca atggcctgaa accgaagtat gaagcgcacc acgacgtgcg ttataccgca
    20161 aaagcggtgc gtgcggcggt agagctggcg gtgaaataca ttaacgatcg tcatctgccg
    20221 gataaagcga ttgatgttat cgacgaagcg ggcgctcgcg cacgcctgat gccggtaagc
    20281 aaacgcaaga aaaccgttaa tgtggcggat attgagtccg tggtggcccg tattgcacgc
    20341 attccagaga agagtgtttc tcagagtgac cgcgataccc tgaaaaacct cggcgatcgc
    20401 ctgaaaatgc tggtcttcgg tcaggataaa gccattgagg cgctgactga agccattaag
    20461 atggcgcgtg caggtttagg tcacgaacat aaaccggtcg gttcgttcct gtttgccggt
    20521 cctaccgggg tcgggaaaac agaggtgacg gtacagcttt cgaaagcttt gggcattgag
    20581 cttctgcgct ttgatatgtc cgagtatatg gaacgccata ccgtcagccg tctgattggt
    20641 gcgcctccgg gatacgttgg ttttgatcag ggcggtttgc tgactgatgc ggtcatcaag
    20701 catccacatg cggtgctgct gctggacgaa atcgagaaag cgcatccgga cgtgttcaat
    20761 attctgttgc aggtgatgga caacggtacg ctgaccgata acaacggacg caaagcggac
    20821 ttccgtaacg tggtactggt gatgaccact aacgccgggg tacgggaaac tgagcgcaaa
    20881 tccattggtc ttatccacca ggataacagc accgatgcga tggaagagat caagaagatc
    20941 tttacaccgg aattccgtaa ccgtctcgac aacattatct ggttcgatca tctgtcaact
    21001 gacgtgatcc atcaggtggt ggataaattc atcgtcgagt tgcaggttca gctggatcag
    21061 aaaggtgttt ctctggaagt gagccaggaa gcgcgtaact ggctggccga gaaaggttac
    21121 gaccgggcaa tgggcgctcg tccgatggcg cgtgtcatcc aggacaacct gaaaaaaccg
    21181 cttgccaacg aactgctgtt tggttcgctg gtggacggcg gtcaggtcac cgtcgcgctg
    21241 gataaagaga aaaatgagct gacttacgga tttcagagtg cacaaaagca caaggcggaa
    21301 gcagctcatt aatctgattg tcaggtaggt tggtgaagtc cgtaatctcg aaagaggtta
    21361 cggacttttt atttatgggg tggaggaggt tcagaccctt tttttgatga tgatggttag
    21421 ttgttgataa ttagtgctgc tggaaggtaa ggataaaaaa gggtgctgca ggagaatggg
    21481 atggttttgc tttattaaca acgggctaaa cgtgtagtat ttgaattcac tgccgtacag
    21541 gcagcttaga aagctgtatt cggcaaatga agtggatgtt gatggttcac tgccgtacag
    21601 gcagcttaga aattcacagg taacatactc cacccgccca ccatgttcac tgccgtacag
    21661 acagataaaa tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt aaatatggcg
    21721 gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca ggcgtgctcc
    21781 ttcagccact cggacacctc accaaattgt attgctgcca aacctcatgg gtggcaacgg
    21841 ggcgctacta tagggagttg gagtaaaacg gtcaagaaga attttaatga taattattgt
    21901 ttgctcatac tgtaaacaag ttgtgcagta tatctacatc gagacaagtt atggacttat
    21961 acttccaaag tacttcatac atatcacaaa ataaaaaggc cggttaaacc gaccttttac
    22021 tcgttctttc tcttcgccca tcaggcggta aaacaatcag cgactacgga agacaatgcg
    22081 gcctttgctc aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg tcaggatgcg
    22141 gatgtagttt ttgcgcattt taccggagat gtgtgcagta accacgtgac cgttttctaa
    22201 ctctacgcgg aacatggtat taggcaacgt ttcaagaacg gtaccttgca tttcaatatt
    22261 gtcttctttg gccatctaat cctctggggt atcactaccg taatttgaac cggcaagata
    22321 atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa acaggttcgg
    22381 cacattactc cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat aagggagcgg
    22441 tgagataaac gatgggcgtt acctgacgcg aaaaattcct tatcggcagc ggggtaatga
    22501 gcgtaaccaa ctctgcgacc gcaattataa cactctgggg agaaatgtgc cgaaaacatt
    22561 cattcttgtg gtgaaaacaa gcatcgtggt acccagaaat tattcggcaa tcgtccgagg
    22621 cgcatttgat tgagataatt aaggtaatcc cggcggggaa tttcgcaggc accaagcgat
    22681 gctgtgtgat cgttaaggac ctggcagtcg ataagcttac cgccatgacc gataaattcc
    22741 tcacagaata ccagaagcgc cgttttagac gcattttcca tccggctgaa catggactcg
    22801 ccacaaaata gcgttccctg ggccacgccg tacataccgc cgacaagctc atcttcacgc
    22861 cagacttcaa tggagtgggc atgaccgagt tcgtgaaggc gatggtaggc ttcgaccacg
    22921 ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac agccttcaat gatctgacca
    22981 aaagcgtaat tcatcgtgac acgatagggc gagcgtttat gaaatcgctt catactacgg
    23041 ctgatatgca gtgattctgg ccatagcacc gcgcggggat cgggcgacca ccagaggatg
    23101 gggtcgcctg gagaaaacca cggaaaaata ccacgctggt aagccattaa caggcgcgca
    23161 gggctaagat cgcccccaag tgccagcagg ccgttaggct cacgtaatgc gccttccggg
    23221 gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc aaaactccac
    23281 gcaagtcgga tcgttcaata atagcttaca aaccctgctt gaactggtaa taacgcccct
    23341 gtctggcaag cagttctgcg tgagtacctt gctcaataat ttgcccgttg tccatcacta
    23401 ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt gaccattaac accgttttct
    23461 cacgcatcat ttctgcaagc aattcaagga tctgactttc ggttgtggca tctaagcctt
    23521 cggtaggttc atccagcaac accagtggcg catcatgtaa cagcgcacgg gcgatagcca
    23581 gacggcgcag ttcaccaccg gagagctggc gtccgccttc acctaaccaa ctgttgagac
    23641 ctgcatcctc gagcagcttc tccagaccaa cgcgacgcaa gatctccgac agagcctcat
    23701 cactactgcc aggcgaggcg agtaacagat tatcacgcag cgtggcgcta aacagatgca
    23761 ctcgctgagg aacaacgctg atggtctgtc gtagagccgc ttcattcagg ctggctatgg
    23821 ggctatcgtt aagcaaaatc tcgccctgtt gcgggtccca tgcgcgggtc agcagttgta
    23881 acagtgttga tttgccgcat ccggttcgcc cgagaatcgc tatatgttcc ccggcgttta
    23941 cctgaagaga aatcccttta agtgcctgtt gggattgctc cggataagtg aactgaacat
    24001 cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg aaggtgacct
    24061 ccggtttttg atccgttaag tcagagatac gtacggcaga ggcaatgact tgccccagat
    24121 gctgaaatgc acccgttact ggtgccagtg cttcaaacgc ggctaacgcg caaaagacaa
    24181 acagggcaat taacgcgccg ggttgagcat tgccgccaac gccgccagac gccatccaca
    24241 gcatcaggat caccgctaac gcgccaatga gcagcattat cgcttgcgac aatgcggtca
    24301 gttcagattg acggcgttgc gcttccagcc attgaatttc tgtattctct agttgcgtgc
    24361 gataacgatc gctggcacca aaaatggtca gctcagcttg cccttgcagc caggccgtca
    24421 gttgttggcg atactgtccg cgaagatgag tcagattttg cccggtgctt tttcccgcac
    24481 gataaaacag cggtggcatc aggaaaagcg tcagtaacat aatgccgccc agcgtaaagg
    24541 cgagggtgaa atcaaggaaa cttaacccga ttgtcaccac cataatcacc acaaaagcgc
    24601 ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca tccgccacca
    24661 cgcggttgag caattcgccc tgacgatagc gcgccagtcc ggcaggggag aggggcaaca
    24721 atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg tcgtgactta
    24781 ccagacgttc aaaataacgc ccggcagtac gggtgattgc ggcaccacgc acgcccgcag
    24841 cgggtagcat atagttgaag ctgtacagtc cggcaacccc cgcaaccgct gaggccgaga
    24901 ggaaccagcc ggaaagtgtc aacagaccga tactggcgag cagcgtcaca attgccagca
    24961 caataccaag acttaacatc catttatgac gtttatacag tgccagatag ggtagcaaag
    25021 cgcgcattta aatctcctcc tgacgatggg ccagtaatgt ggcgaatggg ccaccagcca
    25081 cacttaattc cgcgtaacgt ccttgctcaa taatccggcc atcctgcata acccaaatga
    25141 catcccagtc agcaagatct tctaactggt gggtgaccat taacgttgtc tggcgcagag
    25201 aggcggcatt cagcgcctcc attacgcgct gttcactgtg agcatcaagg ctggcagcgg
    25261 gttcatccaa cagtaatagc gaacagggat ttagtaacgc acgggccacc gccacgcgct
    25321 gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac aggcgtatca acgccttgtg
    25381 gcaggagcgg tagaaactcg ctgacccagg cgttatccag cgctgcttgt aattcttgtt
    25441 cgctggcatc aggtcgcgcc agtagtacgt tatcccgcaa tgttgctgcc ggtaattgtg
    25501 ggttttgccc aacccaggag agatgtttac gccatgattc tggtgataaa tcgcgtaatt
    25561 ctatcccgtt gattcgtaat gatccctgat atgagagaaa accagaaagc gcgttcagca
    25621 gtgagctttt acctgaaccg ctgcgaccaa ccaacactgc acgttggcct gctggcaaag
    25681 taaagttcag cggtccggcc agcgttttac cttccggcga cgtgataaac agatcctcgg
    25741 cctcaatggt cagcggatcg gtcaatgcta attccgtctc accgcgctgc ggatgggcga
    25801 gcggggtttc cataaacgtt ttcagactgt cagctgcacc aacagcctgg gctttagcat
    25861 gataaaacgt accgagatcg cgtaatggct ggaaaaactc tggcgcaagg atcagggcca
    25921 gaaaacccgc agccagcgtc acgccagtat cgtagtgacc aaaatccagc tcgccgagat
    25981 aggaaaaacc aaagtagacc gccaccagag caattgacag cgaggtaaaa aattcgagaa
    26041 tgccggagga taaaaacgcc agtcgtagca cttccattgt ccgttggcgg aaatcttccg
    26101 aagcagaacg aatactttca atttcagctt caccacgacc aaaaatacgc aatgtttcca
    26161 tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga aagttacgtc
    26221 ggttagcatc ggcagccccc atcccaacca gcgccataaa caacggaatt aacggtgcag
    26281 tgcccagcag aatgagcgcc gcagcccagt tagaggggaa gatcgccacc acaatcagca
    26341 acggcaccga cactgccagc gccatttgcg gcaggtagcg tgcatagtaa tcatgcatat
    26401 cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc aggtttaccc tgaatccacg
    26461 ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag cggatatgct
    26521 gcccggcgtg ataacccacc cgttcgcgta accagaccac ccatgcgcgc agtacaaagg
    26581 tcagaaccag taacgtaaag ggaagcagca gggcttcacg gggaatattc tccataatca
    26641 tatgttgcag aattcgcgcc atgaaccagg cctgggcaat gatcaatatg ccgcttacaa
    26701 agcccagcag acgagaaata ttcagccaac gttgggagat gacgctttgc tgttttaacc
    26761 agcgggttaa ctctttttga cgagatttat tcattgcacg cttagcaggt gagttatcag
    26821 aattatttgc agagcaatgt tacaacgggg aaaaaataaa ggcgacccat agtcgcaagg
    26881 tgtcgccttc tttacttttg ttactgattt gtaaaattat tttgcgtcag ctaaaccatc
    26941 gaggtagcgt tccgcatcaa gtgctgccat gcagcctgta ccggccgaag taatggcctg
    27001 gcgataaatg tgatccatca cgtcgcctgc ggcaaagacg ccaggaatgc tggtctgggt
    27061 ggcattacca tgaatacccg actgtacttt gatgtagccg ttttccagtt ccagctgccc
    27121 ttcgaaaatc gcagtattcg ggctgtgacc gatagcaaca aacagaccgg caacgtcgag
    27181 tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga acgccagtga cacccatttg
    27241 atcgccggtc acctcttcca gcgtacggtt ggtgtgcaga atgatgttgc cgttctccac
    27301 tttatccatc agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac ggcggtgaat
    27361 cagatgcact tccgaagcga tgttagacag atacagcgcc tcttcaaccg cggtattgcc
    27421 gccgccgatg accgcaactt tctggttgcg atagaagaaa ccgtcgcagg ttgcacaagc
    27481 agaaacccca cggcctttaa aggcttcttc agagggcagg ccgagatagc gtgcagaagc
    27541 tccggtggca ataatcagcg cgtcgcaagt gtattcgccg ttatcgccat tcagacggaa
    27601 cggacggttt tgcagatcca ccttgttgat atgatcaaaa atgatctcag tttcaaactt
    27661 ggtggcatgt tcgtgcatgc gctccattaa taacggaccg gtcagatcgt ttggatcgcc
    27721 aggccagttt tccacttccg tggtggtggt cagttggccg cctttttcca tgccggtaat
    27781 cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc ccgccgggcc
    27841 tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc atgagatccc catagttgtt
    27901 ggcagacaat gggcaggatt gtaggaaatt tacagacgta aaaaaagagt atgacgattt
    27961 tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc cgtcattatc
    28021 acctctgcta cttaaatttc ccgctttata agccgattaa atgatgaata aacgcccctg
    28081 ttaatgaata tctggcatgt tgtactaaaa atcgatgttt tgctttgaca atcccctggt
    28141 gttttgcgaa aacattcgag gaagaaaaaa aacagtattc ttatatgcgc ataaccatgc
    28201 atgtaaatac catgtttacc gtgctagtga aatctacgta tggcgtggac agacgccatt
    28261 cgtgatgtcg atagctgcca caaggcaacg gtcttctcac cgtagaccca ggcattgcgc
    28321 gccgtgaatc ttcatgattt cggtctatcg tgacgggtag cgactctgaa cagtgatgtt
    28381 tcagggtcag acaggagtag ggaaggaata cagagagaca ataataatgg tagatagcaa
    28441 gaagcgccct ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg agttgcaaaa
    28501 ggatgggcgt atttctaacg tcgagctttc taaacgtgtg ggactttccc caacgccgtg
    28561 ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt cagggctata cggcgctgct
    28621 taacccccat tatctggatg catcacttct ggtattcgtt gagattactc tgaatcgtgg
    28681 cgcaccggat gtgtttgaac aattcaatac cgctgtacaa aaacttgaag aaattcagga
    28741 gtgtcattta gtatctggtg atttcgacta cctgttgaaa acacgcgtgc cggatatgtc
    28801 agcttaccgt aagttgctgg gggaaaccct gctgcgtctg cctggcgtca atgacacacg
    28861 gacatacgtt gttatggaag aagtcaagca gagtaatcgt ctggttatta agacgcgcta
    28921 acacggaaca ggtgcaaaat cggcgtattt tgattacact cctgttaatc catacagcaa
    28981 cagtactggg gtaacctggt actgttgtcc gttttagcat cgggcaggaa aagcctgtaa
    29041 cctggagagc ctttcttgag ccaggaatac actgaagaca aagaagtcac attgacaaag
    29101 ttaagtagcg gacgccgcct tctggaggcg ttgctgatcc ttattgtcct gtttgccgtc
    29161 tggttgatgg ctgccttact aagctttaac ccttcggacc ccagctggtc gcaaacggcc
    29221 tggcatgaac ctatccataa tttaggtggg atgcccggtg cgtggttggc agatacgctg
    29281 ttctttattt ttggcgtgat ggcttacacc attcccgtca ttattgtcgg cggttgttgg
    29341 tttgcctggc gtcatcagtc cagcgacgaa tacattgatt attttgccgt ttcgctacgc
    29401 atcattggcg ttttggcgct catccttacc tcctgtggtc tggcggcaat caacgctgac
    29461 gatatctggt attttgcctc cggtggcgtc attggcagct tactaagcac tacgctacaa
    29521 ccactgctac acagtagcgg gggaactatt gcgctgctct gcgtttgggc agcgggcctg
    29581 acgttgttca ccggttggtc atgggtgacc attgctgaaa aactcggcgg ctggatttta
    29641 aacattctca ccttcgccag taatcgtacc cgtcgcgatg atacctgggt cgatgaagat
    29701 gaatatgaag atgacgaaga gtatgaagat gaaaaccacg gcaaacagca tgaatcacgc
    29761 cgtgcccgta ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga aaaattcatt
    29821 aatccgatgg ggcggcaaac agatgctgcg ctgttctccg gtaagcggat ggatgatgaa
    29881 gaagatatta cctacactgc acgcggtgtg gctgccgacc ctgacgacgt cctattttcg
    29941 ggcaatcgtg caacgcagcc agaatatgac gaatacgatc cattattaaa tggtgcgcca
    30001 attaccgaac ctgtcgctgt agcagctgct gctaccacgg cgacacaaag ctgggctgcg
    30061 ccggttgaac ctgtgactca gacgccgcct gttgcctctg ttgatgttcc accttcgcaa
    30121 cctacagtag cctggcagcc tgaaccgggt ccacaaacgg gagagccggt tattgctcct
    30181 gcaccggaag gttacccaca gcagtcacaa tatgcgcagc ccgcagtgca atataatgag
    30241 ccgctgcaac aaccagtaca gccgcagcag ccgtattatg cacctgcagc tgaacaacct
    30301 gcgcaacaac cttactacgc tcctgcggcg gaacaacctg ttcagcaacc gtattacgca
    30361 actgcgccgg aacagcctgc acaacagccg tattatgccc ctgtgccaga acaaccggtg
    30421 gcaggtaacg cctggcaagc cgaagagcag caatccactt ttgctccaca gtctacatac
    30481 cagactgagc aaacttatca gcagccagcc gctcaggagc cgttgtacca acagccgcaa
    30541 cctgttgaac agcagcctgt tgtggagcct gaacccgttg tagaagagac aaaacccgcg
    30601 cgtccgccgc tttactactt tgaagaagtg gaagagaagc gagcccgtga acgtgaacaa
    30661 cttgcggcct ggtatcaacc gattccagaa ccggttaaag aaccagaacc gatcaaatct
    30721 tcgctgaaag caccttctgt tgcagcagta cctccagtag aagccgctgc cgctgtttct
    30781 ccgctggcat ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc aaccgttgcc
    30841 gcgccagtct tcagtctggc aaatagcggt ggaccgcgtc cgcaggtcaa agaggggatt
    30901 ggcccgcagt tgccacgacc gaaacgtatc cgcgtgccaa ctcgtcgtga attggcgtct
    30961 tacggtatta agctgccctc acagcgtgcg gcggaagaaa aagcccgtga agcccagcgc
    31021 aatcagtacg attctggcga tcagtacaac gatgatgaaa tcgatgcgat gcagcaggat
    31081 gaactggcac gtcagttcgc ccagacacag cagcaacgct atggcgaaca gtatcaacat
    31141 gatgtgcccg taaacgcaga agatgcagat gctgcggcag aggctgaact ggctcgtcag
    31201 tttgcgcaaa ctcaacaaca acgttattcc ggcgaacaac cggctggggc gaatccgttc
    31261 acgctggatg attttgaatt ttcaccaatg aaagcgttgc tggatgatgg tccacacgaa
    31321 ccactgttta cgccaattgt tgaacctgta cagcagccgc aacaaccggt tgcgccacag
    31381 cagcaatatc agcagccgca acagccggtt gcgccgcagc cgcagtatca gcagccacaa
    31441 cagcaggttg cgccgcagcc acaatatcag cagccacaac agccggttgc gccacagcag
    31501 caatatcagc agccgcaaca gccggttgcg ccgcagccgc agtatcaaca gccgcaacaa
    31561 ccggttgcac cgcagccgca gtatcagcag ccacaacagc cagttgcgcc acagccgcag
    31621 gataccctgc ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt gcataaaccg
    31681 acgacgccgc tgccttctct ggatttgctg acaccgccgc cgagcgaagt ggagccggta
    31741 gatacctttg cgcttgaaca aatggctcgc ctggtggaag cgcgtctggc tgatttccgt
    31801 attaaagccg atgtcgtcaa ttactctccg gggccagtta tcactcgctt tgaacttaac
    31861 ctggctcctg gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct tgcccgttca
    31921 ctttcgacgg tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtaggtctg
    31981 gagttaccaa ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga taacgccaaa
    32041 ttccgcgata atccgtcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg
    32101 gtggttgccg atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt
    32161 aaatctgtcg gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat
    32221 gtgcgtttca tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcattccg
    32281 catctgttaa cggaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt
    32341 gttaacgaga tggagcgtcg gtataaactg atgtctgcgc tgggtgtgcg taatctggcg
    32401 ggttataacg aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac
    32461 tggaagccgg gtgacagtat ggatgctcag catccggtgc tgaaaaaaga gccatacatt
    32521 gtggtgttgg tggacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag
    32581 ctgatagcac gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaact
    32641 cagcgtccat cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc
    32701 gcctttaccg tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa
    32761 tcactgctgg gtatggggga tatgctctac tctgggccga actccacgtt gccggtacgt
    32821 gtccatggtg cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg
    32881 cgtggtcgcc cacagtatgt tgatggcatc acctccgaca gcgaaagcga aggtggtgcg
    32941 ggtggtttcg atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc
    33001 actgaaaaac gcaaagcgtc aatttctggc gtacagcgtc agttccgcat tggttataac
    33061 cgtgcagcgc gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac
    33121 aacggtaatc gtgaagtgct ggccccaccg ccgtttgact aattaatgca ttgccggata
    33181 aggcgcggta gcgtcgcatc cggcactcta tcaactgaaa attcagtatt ttcttctttc
    33241 ctcaagctga ttattagcct ggaatagaga gtagagggaa ctcccggtcg ggagtgacgt
    33301 aatttgagga ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt
    33361 agcaagcagc gtttgggctg atgccgcaag cgatctgaaa agtcgcctgg ataaagtcag
    33421 cagcttccac gccagcttta cacaaaaagt gactgacggt agcggcgcgg cggtgcagga
    33481 aggtcagggc gatctgtggg tgaaacgtcc aaacttattc aactggcata tgacacaacc
    33541 tgatgaaagc attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga
    33601 gcaagctacg gcaacctggc tgaaagatgc caccggtaat acgccgttta tgctgattgc
    33661 ccgcaaccag tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt
    33721 cctgacgccg aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga
    33781 tggcacaatc catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcaact
    33841 gaaatcccag caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg
    33901 cgtcacggta gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt
    33961 tttcggataa tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt
    34021 atatcggtca gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct atcgaagccg
    34081 ggcatttaca ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg
    34141 aagtgattgc ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggcg
    34201 tgaaagagat tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca ggtcgccgca
    34261 ctattctttt tgttgacgaa gttcaccgtt tcaacaaaag ccagcaggat gcatttctgc
    34321 cacatattga agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg
    34381 agcttaattc ggcactgctt tcccgtgccc gtgtttatct gttgaaatcc ctgagtacag
    34441 aggatattga gcaagtacta actcaggcga tggaagacaa aacccgtggc tatggtggtc
    34501 aggatattgt tctgccagat gaaacacgac gtgccattgc agaactggtg aatggcgacg
    34561 cgcgccgggc gttaaatacg ctggaaatga tggcggatat ggccgaagtc gatgatagcg
    34621 gtaagcgggt cctgaagcct gaattactga ccgaaatcgc cggtgaacgt agcgcccgct
    34681 ttgataacaa aggcgatcgc ttttacgatc tgatttccgc actgcataag tcggtacgtg
    34741 gtagcgcacc cgatgcggcg ctgtactggt atgcgcgaat tattaccgct ggtggcgatc
    34801 cgttatatgt cgcgcgtcgc tgtttggcga tagcgtctga agatgtgggt aacgctgatc
    34861 cacgagcgat gcaggtggca attgcggcct gggattgctt tacccgcgtt ggcccggcgg
    34921 aaggtgaacg cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg
    34981 ctgtctacac tgcgtttaaa gccgcgctgg ccgatgctcg cgaacgcccg gattatgacg
    35041 tgccggttca tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg
    35101 aatatcgtta cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc
    35161 cggaaatagc acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg
    35221 gcgaaaagct cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt
    35281 aatgttatcg ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat
    35341 ctcccattta attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg
    35401 agccagacgc agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc
    35461 tgggcgctct tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg
    35521 agcgtaactc ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc
    35581 ctttacgtct ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg
    35641 atgctttaca ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg cctgcagatg
    35701 aagtgccggt aggtaaagac gaaaatgaca acgttgaagt cagccgctgg ggtaccccgc
    35761 gtgagtttga ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac tctggcctcg
    35821 actttgcagc tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa gggcagattg
    35881 ctcgcatgca ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct
    35941 acagtgagaa ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc
    36001 tgccgaaatt tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca
    36061 gtaactatgc gctgatccca acggcagaag ttccgctgac taacctggtg cgcggtgaaa
    36121 tcatcgatga agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg
    36181 aagccggttc atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag
    36241 ttgaaatggt gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg
    36301 gtcatgcaga aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgca
    36361 ctggcgacat gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac
    36421 agaacaccta ccgcgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta
    36481 tgcaggcacg ttgtcgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg
    36541 gttctggtct ggctgttggt cgtacgctgg ttgcggttat ggaaaactat cagcaggctg
    36601 atggtcgtat tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg
    36661 gctaatacct aatttttctg aatctaaaaa gcgcctgcgg gcgctttttt tgtctccctt
    36721 tgataccgaa caataattac tcctcactta cacgtaatac tactttcgag tgaaaatcta
    36781 cctatctctt tgattttcaa attattcgat gtatacaagc ctatatagcg atctgctata
    36841 gaaataatta cacaatacgg tttgttactg gaatcaatcg tgagcaagct tgagtgagcc
    36901 attatgaaaa cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg
    36961 gtaaaaacga cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt
    37021 agtcggattg cgcacgctgt cgatagcgcc attccaacaa aatcagacga aaaggttatc
    37081 tggagcgcct gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac
    37141 ggtgaaatca aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac
    37201 caggttcgcg cctgtttgcg tgggcgttcc atgcgtcgcc gtgtctataa tccggaccgc
    37261 ctgaaatatc cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc
    37321 tgggaagaag cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc
    37381 aacgaatcca tctatcttaa ctatggcacc ggtacgctgg gtggcaccat gacccgctcc
    37441 tggccgccgg gaaataccct ggtcgcgcgg ctgatgaact gctgcggcgg ttatctgaac
    37501 cattacggtg actactcttc cgcgcaaatt gcggaaggtc tgaactatac ctacggcggc
    37561 tgggcagatg gcaacagccc gtcggatatc gaaaacagta agctggtagt gctgtttggt
    37621 aataaccctg gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggcg
    37681 cgtcagaaat ctaatgcccg catgatcatc atcgatccgc gctataccga caccggtgcc
    37741 gggcgcgaag atgagtggat ccctattcgt ccaggaacag atgccgcact ggttaacggt
    37801 ctggcgtatg tcatgatcac cgaaaacctg gtggatcagg cattcctcga taaatattgc
    37861 gttggctatg acgagaaaac cctgccagcc agtgcgccga aaaatggcca ctataaagct
    37921 tatattctgg gtgaagggcc agatggcgtg gctaaaacac cggaatgggc ctcgcaaatc
    37981 accggtgttc cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg
    38041 gcgtttatca gccagggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt
    38101 gctatctcga tgctggcgat tctgaccggt aacgttggta ttaatggagg gaacagcggc
    38161 gcgcgtgaag gttcatacag cttaccgttt gtccgtatgc cgactctgga aaacccgatc
    38221 cagaccagca tttcgatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgacg
    38281 gcgctgcgtg atggtgtgcg cgggaaagat aagctggatg tgccgatcaa aatgatctgg
    38341 aactatgccg gtaactgcct gattaaccag cattctgaaa tcaaccgtac ccatgaaatc
    38401 cttcaggatg ataagaagtg cgagctgatt gtggttatcg actgccacat gacttcatcg
    38461 gcgaaatatg ctgacatcct gctgcctgac tgtaccgctt ccgaacagat ggactttgca
    38521 ctggatgcat cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg
    38581 cgctttgaat gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt
    38641 gagcaacagt ttactgaagg ccgtacccag gaagagtgga tgcggcatct gtatgcccag
    38701 tcgcgggaag cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt
    38761 aaaaagcgcg acccacaagg gcatcacgtt gcttataaag ccttccgtga agatccgcag
    38821 gcaaacccac tgactacgcc atcgggcaaa attgagattt attcgcaggc gctggctgac
    38881 attgccgcta cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctacacg
    38941 ccgggctttg aaagttatca ggatccgctg aacaaacagt atccgctgca gcttacgggt
    39001 ttccactata aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcggct
    39061 tgccgtcagg aaatgtggat caacccgctt gatgcccaaa aacgcggtat ccacaacggc
    39121 gataaagtca ggatctttaa cgatcgtggt gaggttcata ttgaggcgaa agtgacgcca
    39181 cgaatgatgc cgggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatgcaaaa
    39241 cgtgtcgata agggtggttg tatcaacgta ctgaccactc aacgtccgtc tcctctcgct
    39301 aaggggaatc cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat
    39361 gacaacccag tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga
    39421 gctggcttgt aaagactaca aagatttgac accagaagtc agcttccgcc gcatttgtga
    39481 atatgctggt ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta
    39541 ctatctgtcg atttcgtgca accactgcga agatccggct tgtactaaag tctgcccgag
    39601 cggtgcgatg cataaacgtg aagatggttt tgttgtggtc gatgaagatg tgtgcattgg
    39661 ctgccgctac tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg
    39721 ccatatgacc aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg
    39781 tgttgaatct tgtccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa
    39841 acatggcgat ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat
    39901 tgtgatcaaa cccaatgcca atagccgccc gaccggggat accactggct atctggcaaa
    39961 cccgaaggag gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg
    40021 tcttcgggca atgtgtggcg ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg
    40081 acctgcgagc agaaacccag cagcgtgtta tcgcctgcat gtttggctta tgggtgctga
    40141 tgggcattgg ctttatcgcc tctatgctcc atcttggttc accgatgcgc gcctttaact
    40201 cgctcaaccg ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt
    40261 ttgctgtagg cggcatcggc tggttgctgg caatgctgaa aaagctgtca ccggcattgc
    40321 gtacgctgtg gctggtagtg acgatggttc ttggcgtagt ctttgtctgg atgatggtgc
    40381 gtgtctataa cagcattgat accgttccga cctggtacag catctggacg ccgatgggct
    40441 tcttcctgac gatgttcatg ggcggcccgt tactgggtta cctgttgctg agtctggctg
    40501 gcgtcgatgg ctgggcgatg cgtctgctgc cagccatttc tgtacttgca ctggtagtga
    40561 gtggcgtgat gtcagtgatg cagggcgcag agctggcgac tattcatagt tctgtgcagc
    40621 aggccgcagc gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg
    40681 ctgttgccct gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc
    40741 tactatccgt ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct
    40801 atggtttgca tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt
    40861 cggccccggt tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac
    40921 gataatcagg aatatggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt
    40981 cacggtgcag ctcgcaagcc acgccgaacc atgtcatcaa ttgcgcacct gcttgcgaca
    41041 tgcgatccca tgccgaatgc cgggtaattt cattaaaggt ccctgatgcg tcagtcacga
    41101 caaacacatc aaacccctct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg
    41161 ttaccacacc ggcaataatt aactgttttt tacctgtcgc tttgactgct tttacaaaat
    41221 cttcgttatc ccaggcgtta atatttcccg ggcgagcaat gtaaggtgca tcggggaatt
    41281 gtgctttcag ttctggaact aatggaccgt taggaccggt ttcaaaactg gtggtaagaa
    41341 tagttggtaa gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact
    41401 tatcgggttc gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa
    41461 cggcagcatc atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag
    41521 agtcagtggt acgtctgaaa tcggtcagac gcgatgcatt gctctgaaag catagacggg
    41581 aaatatgagt ttgctgtgac catgaaattt ttcgactgaa cgaagcttat gaaatttgtg
    41641 ttagatcaat aaaaacaatc agatgagatt aatcatctct tcagtattag cgttatgaat
    41701 gagtaatata aatcgataat accgccgttg cgatatgcgc gtattttcac ccattgacaa
    41761 tgtttttggc ggtggcatga tgcgcatgaa atttgaactt cctcacggtt ttaattcatg
    41821 tccacgtata cccggcctgt catgcttttg ctgtctggcc tgcttttgtt gactctggcg
    41881 atcgcggtgt taaatacact cgtgccgctt tggctcgccc aggaacacat gtccacatgg
    41941 caggtaggcg ttgtcagctc atcctatttt accggcaacc ttgtcggtac attgctgaca
    42001 gggtatgtca ttaaacgcat tggctttaac cgcagctatt atctggcctc cttcattttt
    42061 gctgctggct gtgccggcct tggcctgatg attggtttct ggagctggtt ggcgtggcgt
    42121 tttgtcgctg gcgtcggctg tgccatgatt tgggtggttg ttgagagcgc gctgatgtgc
    42181 agtgggacgt cacgtaaccg tgggcgtttg cttgctgcgt atatgatggt ttattacgtg
    42241 ggaacgtttt taggccagtt actggtcagc aaagtctcaa ccgagctgat gagcgtattg
    42301 ccgtgggtta caggtttgac gttggcaggg atcttaccgc tgttgtttac acgtgtcctg
    42361 aatcagcagg ctgaaaacca tgattcaacg tcaattacgt ctatgctaaa actccgtcag
    42421 gcgcggcttg gcgtgaatgg ctgcattatc tcaggaatcg tgctgggatc tctatatggc
    42481 ctgatgccgc tgtacctcaa tcacaaaggg gtgagcaatg ccagcattgg tttctggatg
    42541 gcggtactgg tcagtgcggg tatcctcgga caatggccga ttggacgtct ggcggataag
    42601 tttggtcgac tgctggtgtt gcgtgttcag gtctttgtcg tcattctcgg cagtatcgcg
    42661 atgcttagcc aggcggcgat ggccccagcg ttattcatcc tcggtgccgc tggctttacg
    42721 ctatatccgg tggcgatggc ctgggcttgt gagaaagttg aacatcatca actggtggcg
    42781 atgaaccagg ccttactgtt gagctatacc gtgggaagtc tgcttggccc gtcatttacc
    42841 gctatgctaa tgcagaattt ctccgataat ttattgttta tcatgatcgc cagcgtatcg
    42901 tttatctatt tgctgatgct gcttcgcaac gccggtcata cgccgaaacc cgttgctcac
    42961 gtgtaaatga attcaagcag agtgtgaatt tactgtttca cactctgctt ttttgtttct
    43021 tctatctgac ttgctttttt ccaaatttta ttcgtttaaa aataaaatgt gcagcaggtt
    43081 ataattttgc atttcgctat ttccgcactt cttatttgcc gcgcataatc cctcgtttta
    43141 cagatgcccc tttaattttg gcgaaggatt tgtctatggc tgggaatgtt caggaaaaac
    43201 agctgcgatg gtacaacatt gcgctgatgt cttttatcac tgtctggggt tttggcaacg
    43261 ttgttaataa ctatgccaac caggggctgg tggttgtttt ttcatgggtg tttatctttg
    43321 cgctctattt cacaccttat gcgctaattg ttggtcagtt aggctcgacc ttcaaagatg
    43381 ggaaaggcgg ggtcagtacc tggattaaac acacgatggg acccggactg gcttacctcg
    43441 ccgcgtggac ctactgggtg gtgcatattc cctatctggc acaaaaaccc caggcaatac
    43501 tgattgcgct cggttgggcg atgaaaggcg acggttcgct aatcaaagaa tattcagtcg
    43561 tagcgttaca ggggttaacg ctggtgctgt ttatcttctt tatgtgggtt gcttcacgcg
    43621 gtatgaaatc gctgaaaatc gtcggttctg tggcagggat tgcgatgttt gttatgtcgc
    43681 tcctgtatgt ggcgatggcg gtaaccgcgc ctgcgattac tgaagtgcat attgcgacca
    43741 caaacattac ctgggaaacg ttcattcctc atatcgactt tacctacatt accactattt
    43801 caatgctggt tttcgcggtt ggcggagcag agaagatttc tccttacgtt aatcaaacgc
    43861 gcaacccagg aaaagaattt ccaaaaggga tgctatgcct ggcggtgatg gttgcggttt
    43921 gtgccattct gggctcactg gcgatgggga tgatgtttga ctcgcgtaat atcccggatg
    43981 acttaatgac taacggtcaa tattacgctt ttcagaagct gggcgagtat tacaacatgg
    44041 gtaatacttt aatggtgatt tacgccattg cgaataccct gggacaagta gctgcgctgg
    44101 tattctcgat tgatgccccg cttaaagtgc tattaggtga tgctgacagc aaatatattc
    44161 cagccagttt atgtcgtacc aacgcttctg gtacgcccgt taatggctat tttctgaccc
    44221 tggtgctggt ggcaattctg attatgctgc cgactctcgg catcggtgat atgaacaatc
    44281 tctataaatg gctgttgaat cttaactcgg tagtgatgcc gctgcgttat ttatgggtat
    44341 ttgttgcatt tattgcagtc gttcgcctgg cgcagaaata taaaccagaa tatgtcttta
    44401 ttcgtaataa gcctctggca atgaccgtcg ggatttggtg ttttgccttt accgcctttg
    44461 cctgtttgac ggggatcttc ccgaaaatgg aagccttcac tgcagagtgg accttccagt
    44521 tggcgctgaa tgttgcaacg ccgtttgtgc tggtaggatt aggactgata ttcccgctgc
    44581 tggcgcgtaa agcgaatagt aaataattat tgtggtcggt gacgctgact ctggcagatc
    44641 tgacccgttt ttacataatc gagaaatgcg cgcaagccgc aggacatata ctggcggttt
    44701 gggtaataca tttggaagct gtgctgtaag ggcgttagaa atagagctct gaattattta
    44761 tgcagcgcgg aataaaaaag cggcaactca ataaagttgc cgctttacgg ggaaattaga
    44821 acattacctt atgaccgtac tgctcaagaa tgcctttcac gcgttccatg gtttctttct
    44881 tcggtggttt aacaccatcg agtttgtatt cttcacccat cgccacccat ttgtgtttgc
    44941 ccagttcgtg gtaggggaga agttcgattt tctcaacgtt acccatatca cgggtaaatt
    45001 caccgaggcg atgcgctgaa tcgtcatcgt cagaccagcc tgggacaaca acgtagcgga
    45061 tccacacctt cacatttttg ttcgccagat atttagcgaa ctccagcgtg cggtggttgg
    45121 aaactccaac cagattttgg tggatctcgt cgttcatctg tttgagatcg agcattacca
    45181 ggtcggttac ttccagcagt tcatcaatca ccggatcgta acgacgaaca aaaccgttgg
    45241 tatccagaca ggtatgaatg ccttcttttt tacaggcgcg gaaccagtca cgaacaaact
    45301 cagcttgcag gattgcttca ccgccggatg cggtaacgcc gccgccggaa gcgttcataa
    45361 agtggcgata ggtcaccact tccttcatca aatcttcaac ggtaacttct ttaccgccat
    45421 gcgtgtccca ggtgtcgcgg ttatgacaat acaggcagcg catcaggcag ccctggaaaa
    45481 aggtgataaa gcgaataccc gggccgtcta cggttccaca ggattcaaag gagtgaatgc
    45541 gaccaataac tgacattgcg gtgtttctcc agatgtggcc catctgaggc cgtgttggtg
    45601 cgcagctcga aggctacgtc gagtctgttt tggcagtcac cttaaagtat agatagctga
    45661 caaaaaaggc tctcgtgcta aaaaaggccc cactttcgtg gagcctttat tgtacgcttt
    45721 ttactgtacg atttcagtca aatctaatta catagattga gtgaaggtac gagtaataac
    45781 gtcctgctgc tgttctttag tcagcgagtt gaaacgtact gcgtagccag atacacggat
    45841 ggtcagctgc ggatattttt ccgggttttc catcgcgtca agcagcattt cacggttcat
    45901 cacgttaacg ttcaggtgct gaccaccttc gatggatgct tcgtggtgga agtaaccatc
    45961 catcagacct gccaggttgg tcttacgaac ttcgtcgtct ttacccagtg cgttcggaac
    46021 gatagagaag gtgtaggaga taccatcttt agcgtaagca aacggcagtt tagcaacgga
    46081 agtcagagac gctacagcac ctttctggtc acgaccgtgc atcgggttag cacccggtcc
    46141 gaacggcgcg ccagcacgac gaccgtctgg ggtgttaccc gttttcttac catacacaac
    46201 gttagaagta atggtcagaa cagactgagt cgggatagcg tcacggtagg tatgcagttt
    46261 ctgaattttc ttcatgaaac gttctaccag gtcaacagcc aggtcatcta cacgcggatc
    46321 gttgttacca aactgcgggt attcgccttc gatttcgaag tcgatagcca gaccgtcttc
    46381 gtcacgaatc ggtttaactt tcgcatattt gattgcagac agggagtcag cagcaacgga
    46441 cagaccagcg ataccacacg ccatggtgcg gataacgtca cggtcgtgca gcgccatcag
    46501 agaggcttcg tagctgtact tgtcgtgcat gtagtgaatg atgttcagtg cagtgatgta
    46561 ctgtttagcc agccagtcca tgaagtgatc catgcgctcc atcacttcat cgtagttcag
    46621 gagatcgcct ttgatcggtt cagacttcgg accaacctgc attttcagtt tttcgtcaac
    46681 gccgccgttg attgcgtaca gcatggtttt cgccaggttt gcacgcgcac cgaagaactg
    46741 catttgttta ccaacgatca tcgggcttac gcagcaagcg atagcgtagt catcgttgtt
    46801 gaagtccgga cgcatcaggt catcgttctc atactgcaga gaagaggtgt cgatggacac
    46861 tttagcggcg aatttcttga agttcagcgg cagtttttca gaccacagaa tggtcatgtt
    46921 cggttccgga gacggaccca tggtgtacag ggtgttcagg aaacggaagc tgtttttggt
    46981 aaccagggta cgaccgtcga ggcccatacc accgatagat tcggttgccc agatcgggtc
    47041 gccagagaac agttcatcgt actccggagt acgcaggaag cgaaccatac gcagtttcat
    47101 gaccaggtgg tcaaccattt cctgcgcttc ttgttcggtg atcttgccag ctttcaggtc
    47161 acgttcgatg tacacatcca ggaaggtgga ggtacgaccg aaggacattg cagcaccgtt
    47221 ctgagactta acagcagcca ggtagccgaa gtaagtccac tggatagctt cctgagcgtt
    47281 ggtagccgga ccagagatgt cgtagccgta tttcgcagcc atttctttca tctgacccag
    47341 agcgcggtgc tgttcagcga tttcttcgcg cagacggata gtctgttcca ggtttacgcc
    47401 gttttccaga tcagcctgca gagaggtgaa ctgagcgtat ttgtctttca tcaggtagtc
    47461 gataccgtac agcgcaacgc gacggtagtc accgatgata cggccacggc cataagcatc
    47521 tggcagaccg gtcagaacac cggatttacg gcaacgcagg atgtccggag tgtaaacgtc
    47581 gaacacgccc tggttgtgag ttttacggta ttcagtgaag atttttttga tcatcgggtc
    47641 cagttcgcgg ttgtacgctt tgcaggaacc ttcgatcatt ttgataccac cgaacgggat
    47701 aagagcacgt ttcagcggag cttcagtctg cagaccaaca actttttcca acgctttgtt
    47761 gatgtagcca gcgtcgtgag aggtgatggt ggaagcaaca gcggtgtcaa agtcaactgg
    47821 cgcgtgagtg cggttttcca gtttaacgcc ttccattact ttgtcccaca gggtggtggt
    47881 cgcttcagta gcgccagcca ggaaggactc gtcaccctcg tacggagtgt agtttttctg
    47941 aatgaagtca cggacgttta cttcattctg ccagtcacct ttggtaaaac cttcccaggc
    48001 tgtggctaac ttttcattaa gctcggacat gtaacaccta ccttcttaag tggatttttt
    48061 atttactgcg tacttcgaca accattaatg gtgatcgttt tcacgcaggt aaatgaccca
    48121 gtatgtcaac ccaaccaaca aaccaccgcc gataatatta ccgatcgtaa ccggaatcag
    48181 gttatcagtg atgaaattca tcacggtcag gtgagaaaaa ttttccggtg cagaaccgac
    48241 agcggtccag aattccggag atgcgaagtc gcggattaca atacccatcg ggatcataaa
    48301 catgtttgcg atactgtgct caaaaccgct ggcaacaaac atcgcgaccg gcagcaccat
    48361 gataaacgct ttgtccatca ggctgcggcc agaataactc atccatactg ccagacatac
    48421 catcaggttt gccaggatac caaggcagac ggcctcaata aaagtatggt gcactttgtg
    48481 gtcggcggtt tgtaggacgt ttagtcccca ttgaccattt gcggtcatat actcgccgga
    48541 aagccacatt aaaagtacaa acagcagtgc gccgaccagg ttgccaaaat agacatttag
    48601 ccagttttta gccaactgac cccaggttat gcgcccactc gccttagcaa caacaatcaa
    48661 cactgtggaa gtaaagagat cggctccgca gacaacacaa agaatcagcc ccagagagaa
    48721 gcaaatgccg ccaaccagtt ttgccatgcc gaagggcatt gtgcctgtgc cagtggttgc
    48781 tgtgatatag aagacgaatg cgattgagat gaaaacaccg gcggtaatcg ccagatagaa
    48841 agtcttaagc ggatgtttcg ttgctttata gacacccgcc tcttcggcca ctttggccat
    48901 tgcagcagga agtaaaagat caaaagggtt gtcagctttc acactaactc tctctttatt
    48961 aagtcggcga cgagatacta acaaagcatt atagatgaga aattgatata gatcatatct
    49021 cgcctggctt ataggcccgt
//
LOCUS       DAZUCE010000010        48494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZUCE010000010 DAZUCE010000000
VERSION     DAZUCE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.010"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            95..1072
                     /gene="pspF"
                     /locus_tag="ACHY03_000528"
     CDS             95..1072
                     /gene="pspF"
                     /locus_tag="ACHY03_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFS3903805.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(1203..2468)
                     /gene="puuE"
                     /locus_tag="ACHY03_000529"
     CDS             complement(1203..2468)
                     /gene="puuE"
                     /locus_tag="ACHY03_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFS3903806.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(2506..3786)
                     /gene="puuB"
                     /locus_tag="ACHY03_000530"
     CDS             complement(2506..3786)
                     /gene="puuB"
                     /locus_tag="ACHY03_000530"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFS3903807.1"
                     /translation="MTEHTSSYYAASANKYTPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTTKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(3788..5275)
                     /gene="puuC"
                     /locus_tag="ACHY03_000531"
     CDS             complement(3788..5275)
                     /gene="puuC"
                     /locus_tag="ACHY03_000531"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFS3903808.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLANIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCTHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFKSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(5425..5982)
                     /gene="puuR"
                     /locus_tag="ACHY03_000532"
     CDS             complement(5425..5982)
                     /gene="puuR"
                     /locus_tag="ACHY03_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFS3903809.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(6009..6773)
                     /gene="puuD"
                     /locus_tag="ACHY03_000533"
     CDS             complement(6009..6773)
                     /gene="puuD"
                     /locus_tag="ACHY03_000533"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFS3903810.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            6985..8403
                     /gene="puuA"
                     /locus_tag="ACHY03_000534"
     CDS             6985..8403
                     /gene="puuA"
                     /locus_tag="ACHY03_000534"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFS3903811.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     ILGTLTPSAADPEFIGQILLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            8552..8716
                     /locus_tag="ACHY03_000535"
     CDS             8552..8716
                     /locus_tag="ACHY03_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3903812.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            8706..10091
                     /gene="puuP"
                     /locus_tag="ACHY03_000536"
     CDS             8706..10091
                     /gene="puuP"
                     /locus_tag="ACHY03_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFS3903813.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            10225..10470
                     /gene="ymjA"
                     /locus_tag="ACHY03_000537"
     CDS             10225..10470
                     /gene="ymjA"
                     /locus_tag="ACHY03_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFS3903814.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            10781..12424
                     /gene="sapA"
                     /locus_tag="ACHY03_000538"
     CDS             10781..12424
                     /gene="sapA"
                     /locus_tag="ACHY03_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFS3903815.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPEPTPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            12421..13386
                     /gene="sapB"
                     /locus_tag="ACHY03_000539"
     CDS             12421..13386
                     /gene="sapB"
                     /locus_tag="ACHY03_000539"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFS3903816.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            13373..14263
                     /gene="sapC"
                     /locus_tag="ACHY03_000540"
     CDS             13373..14263
                     /gene="sapC"
                     /locus_tag="ACHY03_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFS3903817.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            14263..15255
                     /gene="sapD"
                     /locus_tag="ACHY03_000541"
     CDS             14263..15255
                     /gene="sapD"
                     /locus_tag="ACHY03_000541"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFS3903818.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            15257..16063
                     /gene="sapF"
                     /locus_tag="ACHY03_000542"
     CDS             15257..16063
                     /gene="sapF"
                     /locus_tag="ACHY03_000542"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFS3903819.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(16114..17277)
                     /locus_tag="ACHY03_000543"
     CDS             complement(16114..17277)
                     /locus_tag="ACHY03_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFS3903820.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCMLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(17286..18659)
                     /locus_tag="ACHY03_000544"
     CDS             complement(17286..18659)
                     /locus_tag="ACHY03_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFS3903821.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDHRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTETTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(18663..21770)
                     /locus_tag="ACHY03_000545"
     CDS             complement(18663..21770)
                     /locus_tag="ACHY03_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFS3903822.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYLVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(21770..22891)
                     /locus_tag="ACHY03_000546"
     CDS             complement(21770..22891)
                     /locus_tag="ACHY03_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFS3903823.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(23040..23606)
                     /locus_tag="ACHY03_000547"
     CDS             complement(23040..23606)
                     /locus_tag="ACHY03_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS3903824.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            24084..24872
                     /gene="fabI"
                     /locus_tag="ACHY03_000548"
     CDS             24084..24872
                     /gene="fabI"
                     /locus_tag="ACHY03_000548"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFS3903825.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSNIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            25016..26143
                     /gene="yciW"
                     /locus_tag="ACHY03_000549"
     CDS             25016..26143
                     /gene="yciW"
                     /locus_tag="ACHY03_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFS3903826.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVDLFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISTLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            26211..28145
                     /gene="rnb"
                     /locus_tag="ACHY03_000550"
     CDS             26211..28145
                     /gene="rnb"
                     /locus_tag="ACHY03_000550"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFS3903827.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVRKDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSGWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFSA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(28223..28327)
                     /locus_tag="ACHY03_000551"
     CDS             complement(28223..28327)
                     /locus_tag="ACHY03_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3903828.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            28381..30366
                     /gene="pdeR"
                     /locus_tag="ACHY03_000552"
     CDS             28381..30366
                     /gene="pdeR"
                     /locus_tag="ACHY03_000552"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFS3903829.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(30348..30476)
                     /gene="ymiD"
                     /locus_tag="ACHY03_000553"
     CDS             complement(30348..30476)
                     /gene="ymiD"
                     /locus_tag="ACHY03_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFS3903830.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            30514..30687
                     /gene="yciZ"
                     /locus_tag="ACHY03_000554"
     CDS             30514..30687
                     /gene="yciZ"
                     /locus_tag="ACHY03_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFS3903831.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            30776..31525
                     /gene="yciT"
                     /locus_tag="ACHY03_000555"
     CDS             30776..31525
                     /gene="yciT"
                     /locus_tag="ACHY03_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFS3903832.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            31794..32012
                     /gene="osmB"
                     /locus_tag="ACHY03_000556"
     CDS             31794..32012
                     /gene="osmB"
                     /locus_tag="ACHY03_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFS3903833.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(32138..32464)
                     /gene="yciH"
                     /locus_tag="ACHY03_000557"
     CDS             complement(32138..32464)
                     /gene="yciH"
                     /locus_tag="ACHY03_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFS3903834.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(32464..33201)
                     /gene="pyrF"
                     /locus_tag="ACHY03_000558"
     CDS             complement(32464..33201)
                     /gene="pyrF"
                     /locus_tag="ACHY03_000558"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFS3903835.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHAFG
                     GARMMTAAREALVPYGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(33394..34563)
                     /gene="lapB"
                     /locus_tag="ACHY03_000559"
     CDS             complement(33394..34563)
                     /gene="lapB"
                     /locus_tag="ACHY03_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFS3903836.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMALLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQICYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(34570..34878)
                     /gene="lapA"
                     /locus_tag="ACHY03_000560"
     CDS             complement(34570..34878)
                     /gene="lapA"
                     /locus_tag="ACHY03_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFS3903837.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(35027..35791)
                     /gene="pgpB"
                     /locus_tag="ACHY03_000561"
     CDS             complement(35027..35791)
                     /gene="pgpB"
                     /locus_tag="ACHY03_000561"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFS3903838.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            35961..36551
                     /gene="ribA"
                     /locus_tag="ACHY03_000562"
     CDS             35961..36551
                     /gene="ribA"
                     /locus_tag="ACHY03_000562"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFS3903839.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(36615..39290)
                     /gene="acnA"
                     /locus_tag="ACHY03_000563"
     CDS             complement(36615..39290)
                     /gene="acnA"
                     /locus_tag="ACHY03_000563"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFS3903840.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPIYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(39454..39549)
                     /gene="ymiC"
                     /locus_tag="ACHY03_000564"
     CDS             complement(39454..39549)
                     /gene="ymiC"
                     /locus_tag="ACHY03_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFS3903841.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(39663..39830)
                     /gene="yciX"
                     /locus_tag="ACHY03_000565"
     CDS             complement(39663..39830)
                     /gene="yciX"
                     /locus_tag="ACHY03_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFS3903842.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECTVVIPDNWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(39833..39973)
                     /gene="ymiA"
                     /locus_tag="ACHY03_000566"
     CDS             complement(39833..39973)
                     /gene="ymiA"
                     /locus_tag="ACHY03_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFS3903843.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            complement(40292..41266)
                     /gene="cysB"
                     /locus_tag="ACHY03_000567"
     CDS             complement(40292..41266)
                     /gene="cysB"
                     /locus_tag="ACHY03_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFS3903844.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(41476..44073)
                     /gene="topA"
                     /locus_tag="ACHY03_000568"
     CDS             complement(41476..44073)
                     /gene="topA"
                     /locus_tag="ACHY03_000568"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFS3903845.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGDALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            44453..44704
                     /gene="yciN"
                     /locus_tag="ACHY03_000569"
     CDS             44453..44704
                     /gene="yciN"
                     /locus_tag="ACHY03_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFS3903846.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(44740..45789)
                     /gene="sohB"
                     /locus_tag="ACHY03_000570"
     CDS             complement(44740..45789)
                     /gene="sohB"
                     /locus_tag="ACHY03_000570"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFS3903847.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            46009..46767
                     /gene="yciK"
                     /locus_tag="ACHY03_000571"
     CDS             46009..46767
                     /gene="yciK"
                     /locus_tag="ACHY03_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFS3903848.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGHQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            46764..47354
                     /gene="cobO"
                     /locus_tag="ACHY03_000572"
     CDS             46764..47354
                     /gene="cobO"
                     /locus_tag="ACHY03_000572"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFS3903849.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(47394..48269)
                     /gene="rluB"
                     /locus_tag="ACHY03_000573"
     CDS             complement(47394..48269)
                     /gene="rluB"
                     /locus_tag="ACHY03_000573"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFS3903850.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
BASE COUNT        11670 a        12802 c        11987 g        12035 t
ORIGIN      
        1 ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc caggatgaat
       61 tagcgaatta cactaacaag tggcgaattt catcatggca gaatacaaag ataatttact
      121 cggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg caccgctgga
      181 caaaccggtg ctcatcatcg gcgagcgcgg aacaggtaaa gagctgatcg ccagccgtct
      241 gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg cggcgttaaa
      301 tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggcgcgttta ccggtgcgca
      361 aaaacgtcat ccagggagat ttgaacgtgc cgacggcggt acgctatttc ttgatgaact
      421 cgctacggca ccgatgatgg tgcaggagaa attattgcgc gtgattgagt acggtgaact
      481 ggagcgcgtt ggcggcagcc agccattgca ggtgaatgtg cggttggtat gcgcgacgaa
      541 tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc tcgaccgcct
      601 ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca ttatgctgat
      661 ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt tcccgggttt
      721 taccgagcac gccagagaaa cattgctgaa ttatcgttgg ccgggaaata ttcgtgaatt
      781 gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc cgcttgatga
      841 catcattatt gatcccttta aacggcgtcc gcctgaagac gctatcgccg tttcagaaac
      901 cacctcgctt ccaacactgc cgctggattt acgtgagttt cagatgcagc aggaaaaaga
      961 gttgctgcaa ctcagtttgc aacaagggaa atataaccag aagcgcgcgg ctgaattact
     1021 ggggttaacc tatcatcagt tccgcgcgtt gttgaaaaag caccagattt agcgaagttg
     1081 ggcaatacaa cttgccggat gcggcgtgaa cgccttatcc gtcatacata atcaatacgt
     1141 cgtttgcagg tctgataagc gcagcgtatc aggcattatt gcgttgcccc tgcttctgcg
     1201 tattaatctc tcagcgcatc ctgcaaaatt ttcattgccg catcgaattg cgcatccggg
     1261 atggtcagcg gatacaggaa acgaatcacg ttgccgtatg cgccacaggt cagtagaagc
     1321 agtccctgcg ccagcgcgcg ttgctggatt ttctgtgcaa tcgccgctga cggctcgccc
     1381 gtttgcggat cgttaaactc tgccgcaatc atagacccca gaccgcgtac cgccgcgatg
     1441 gccggaacgc tttctttggc atcaatcagc gtgtttgtca gacgctggcc cagttgattc
     1501 gcgcgttcgc agagtgattc tttgtcgata atgttgagca ccgcgtgcgc ggcagccacc
     1561 gccagcgggt taccggcgta ggtgccgcca agcccgcccg gcgcgggtgc gtccataata
     1621 ttcgcgttac cgaccacgcc cgaaagcggc atcccgcccg cgaggctttt cgccatcgtc
     1681 attaaatccg gcttatcggc gtaatgatcc atggcaaaca gcttaccggt acgcgcaaag
     1741 ccgctttgca cttcatcagc aatcatcaca ataccgtgct cgtcgcacag gcggcgaatg
     1801 gcggcaacca gctcttttgg cgcaacgttg aaaccgccct cgccctgcac cggttcgaaa
     1861 ataatggccg cgacctgctt cgcttcgatg tctgatttaa acaagcgttc gatggcgtcg
     1921 agggagtcct gtgttgaaat gccgtgtaaa tctgacggat aaggtacgtg atacaccgaa
     1981 ccagggaacg ggccgaagcc gattttgtac ggcgcgacct ttccagttaa cgccatagtc
     2041 atatacgtac gaccgtgaaa gccgccgcta aacgcaatca cgccagggcg tccggtatgg
     2101 gcgcgggcga ttttcaccgc gttttccacc gcttccgcac cggtggtgaa gaacgcggtt
     2161 ttggcctgcc cgctcaccgg ggcaagggcg ttgattttct ccgccagggt gacgtagctt
     2221 tcatatggta caatctgata cgcggtgtgg gtaaactgtt gcagctgctg ctccaccgcc
     2281 gcgaccagat cagggtggcg atgtccggta ttcagcaccg caatgcctgc ggcgaaatcg
     2341 atgtactcgt tgccctcaac atccttcagc gtggcgtttt cagccgactg ggcgaagaag
     2401 ttacacatca cgccaacccc gcgcggggtg gcagaaagac gacgctgatg gaattcattg
     2461 ttgctcatgt ttcacacctt ttgactcttc actgttaatc ggaaattaaa cgcccagacg
     2521 atcgcgcagg ctgtaataag ccgcgcccat cgcggtaaac ggcacacgca gcgtgcgccc
     2581 gccggggaac gggtaatgcg gcagattggc gaaggcgtcg aaacgttcgg cgtcgccgcg
     2641 cagcagttcg gcaatcaaac gtccggcgag atgagtacag gtgacgccgt ggccgctgta
     2701 accctgcatg taatagatgt tggtatcgag gcgaccgaac tgcggcatac gcgacagggt
     2761 cagcaggaag ttgcccgtcc agcgatagtc gattttcacg cccttcaact gcgggaaggt
     2821 tttcagcagt ttcggcacca caagacgctc aacgtcatcc gggtcgcgcg cgccgtacac
     2881 cacgccgccg ccgtacagca aacggttgtc ggcggtaaga cggtagtaat caagcagata
     2941 gttacagtct tccacacagt agtttttcgg gatcagcgaa cgggctaaat cttctgacag
     3001 ccgctcagtg gtgatcacct gcgtgccgca cggcatacta cgcttcgcca gttccggctc
     3061 cactttatcg cccaggtacg cattcccggc gacaatcaca tacttcgccg tcacctggcc
     3121 tttggtagta cgcaccacgg cgggcgtggt gtgctggatc tgcgtcaccg cagaaagctc
     3181 atacacgcgc ccgccgttca ggcggatagc gtccgcttca ccaatggcga ggttcagcgg
     3241 gtgaatatgc ccgccgctgt gatccagcag cgcaccagtg tagcgatcgc tggcgacctc
     3301 gcggcgaatg gcgttggcgt ccagcagctc cagctgttta ttgccgtagc gttcccagtt
     3361 ctctttctgc tcttcgagtg tggcgagctg tttatcattc atcgccacaa acaggccgcc
     3421 ggggcggtag tcgcagtcaa tctgatagcg tttgatacgt tcgcggatga tctcgccacc
     3481 ctcgaacatc atgctgccga gcatacgggc ggtgtccatg ccgtagcttt tttcgatcac
     3541 gtcgatgtcg cggctgtagg agttcacaag ctgcccgccg ttgcgtccgc ttgcgccgaa
     3601 gccaatgcgt gaggcttcaa gaaccaccac gtcaaagccc gcttccgcca gatgtagcgc
     3661 ggaagacagc ccggtatagc cgccgccgac cacgcaaacg tcgcaggtga ttgactcatt
     3721 cagcgtgtcg aatggcgtgt atttattcgc actggcggcg tagtaactgc tggtatgttc
     3781 ggtcatttca ggcctccagg cttatccaga tggttttcag ttcggtgaat ttttcaaggg
     3841 catgcaggga tttgtcgcga ccattgccgc tctgcttata gccgccaaac ggcacggtca
     3901 tatcgccgtc gttgtagtta ttgacgaaga cggaaccggc cttcaggcgg cggctcatac
     3961 ggtgcgcgcg ggagagatcg cgcgtccata ccgccgcgcc gaggccatac tggctgtcgt
     4021 tggcaagctg tagcgcctgg tcttctgatt tgaaacgcgt gaccaccagc accgggccga
     4081 aaatctcttc gcgacttaag gacgcattcg ggtctacatc cacaaagatg gtcgggccga
     4141 tggcggcagc cagcccggcg ttacggccat ccaacaacag ttgccctttg ctttcgcctt
     4201 cccgaataaa gctatggacc gagtcggcgt gggtgcagtc gattaaggtg cccatggtgg
     4261 ttgcggggtc gagcggatgc cccggctgcc agttttgcgc ctgctgtttt aacagggcta
     4321 agaattcatc ggcgatgctc tcttccagca acaggcgcgt tccggcgatg cacacctgac
     4381 cctggttgta gaagatgcct gctgcggtgg cgcttgccgc ctgttgcaaa tccgggcagt
     4441 cagcgaaaac gatgttggcg cttttgccgc ccgcttccag ccagacgcgt ttcatgttgc
     4501 tgtcgcccgc atctttcagc agctgtttcc cggtacgggt tgaaccggta aaggcgatgg
     4561 catcgatatc gttatgacgc gaaagcgcct gccccgcttc atgaccaaaa cccgttacta
     4621 cgttcagtac gccatccggc agacctgctt ctttcgccag cccggcgaga cgaatcgcac
     4681 tgagcggtga tttttcagac ggttttagaa tcacgctgtt tcccgccgcc agcgccgggc
     4741 cgagtttcca gcaagtcagc agcagcggga agttccacgg cacgatggcg gcaattacgc
     4801 cgaccggttc acgcacgatc atcgccagct catggctact ggtggtcgcc acttcgccat
     4861 acactttgtc gatcgcttcg gcgtaccagc gaatggcgcg cgccgcgccg ggaatatcat
     4921 cacgcagact gtgacgaatc ggtttgccgg tgtcgagagt ttccagcagt gccagctctt
     4981 cggcgtgggc ttccattaaa tcggcgagtt tattcagcac cgctttacgt tttgccggag
     5041 aagagagtga ccagtcgccg cgttcaaata cgccgcgtgc tgcgctcacc gcacggtcga
     5101 tatcgacgct cttgccgcgc gcaatattcg ccagcggtgc ctgggtaacc ggatcaacgg
     5161 tttcaaaggt ttcattttcc gccgcagcag tatattcacc gttaataaat aagcgggttt
     5221 caatggcgag acttaacgct ttatcctgcc agtaagccag atgatgaaaa ttcattatga
     5281 ctcctgtttc acgtctatca gatatatgca gtttgtgaac gtcagggcgc agagatcccc
     5341 gggccgtatg cccgtttatt tctggcgaca ttctttatgg attgccgggc gcattgcgct
     5401 tacccggctt acagaacaaa aagattaaaa cgtggtgggc gtatgggcgc tgataattcg
     5461 gcaaatacct gccgacgtat tactgaaact gtgcgggatg ccggtattaa tggcataact
     5521 ttgccccgcg acgaggtggt aatcctgacc attaatcgtc agaacaattt caccttccag
     5581 tacagtgcct atttcctcac cctgatgctt aattctttcc ccagtggttg tgcccggctg
     5641 gtacgtttca aagatcatcg ccagcgtgcg attcgggtta ccgttatgaa ccagcttcat
     5701 cgacacaccc tgactgccca tttcaattaa gtcgtcctga ttaatgacga cctgcggctc
     5761 atcaggtttt tccggctcgg aaaagaattc cgagagtgac agaccataca ccttcagcag
     5821 cttttgcagc gtactgatgg cagggctgac tttatcttgt tctatcgtac tgatagcact
     5881 gtgagtcagc ccggagagtt cggcggcacg acgttgtgaa agcccctgct gctggcggat
     5941 ttccgacaag cgttttcctg gcgccagtcc ctcatcactc atatttgcat ttccttaaac
     6001 tgtagtggtc agagtcgctg tttttcagcg atatggtgct gacaagcggt gataaagccc
     6061 tcgaacaata tacgcgaaag cgcgtactcg ctactgttcc attccgggtg ccactgtacg
     6121 cccagcgcaa aaggatgatt gatgacgcta accgcctcaa ctaagccatc cggcgagcga
     6181 gcttcaacac gcaaccgtgg gctaacgacc ttcgcccctt gtccatgcag agagtttacc
     6241 caaaagttgc tacattcagg taacaacgca gacagtaatc ccccctcttc aacctgaact
     6301 tcatgcgacg gtgcatattg ctgttccacc ggcagttcgg gatcttcccg atgttccagc
     6361 aattcaggct gttcgcacag cttgcgatgc aacgaaccac cggttgccac cactagttcc
     6421 tgtaaacccc ggcagatggc gaaaatgggg atgcgccttt cgagtgcggc attaattaac
     6481 gccatgctca gaagatcacg cccgggatcg gcgtcaggct catcgccgtt ttcaccatat
     6541 aggtgcggct gcacattgct gggactacca ggaagataaa tgccatcgag tttcggcaaa
     6601 agttgttcaa gtaaggacgg ttccgccagc gcatgtggca gcgcaattgg caagccgcct
     6661 gcatggatga tggcattcag gtacttttct tgcagagtct gggtcgcatg acccttaagc
     6721 ctgttcctgc acattacaac accgataacc ggattgttca ttatattttc catgctcaat
     6781 ctcacaaagt ggactaaatt atcgccatta ctgcggttta aatgaccttt atgttcaata
     6841 ttttttcaat ctagcagtgg gttttcattt ttgcaaactc aatttaacat ttgacaaaca
     6901 tttagtttgc atacagattc gaatggtggt cattatattt tacgctttga taacgagcgg
     6961 aaaacaaacc aaaggcgaag aatcatggaa accaatatcg ttgaagtaga gaactttgtt
     7021 cagcagtcag aagagaggcg gggtagcgcc tttacgcagg aagtgaaacg ctacctggag
     7081 cgctacccga atacgcaata tgttgatgtt ttacttaccg atttgaatgg ttgcttccgt
     7141 ggaaaacgta ttcctgtttc aagcctgaag aagctcgaga aagggtgtta tttcccggcc
     7201 tcggtgtttg caatggatat tctgggcaac gtagtagaag aagcgggcct gggtcaggaa
     7261 atgggtgaac cagatcgtac ttgtgtgcca atactcggta ccttaacccc ttctgccgcc
     7321 gatccagagt ttatcggtca gatactcctg accatggtcg atgaagatgg cgctcccttt
     7381 gacgttgagc cgcggaacgt acttaaccgc ctgtggcagc agctacgcca gcgcggattg
     7441 ttcccggttg tagcggtaga gctggagttc tatttactgg atcgtcagcg cgacgctgaa
     7501 gggtatctgc agccgccctg cgcgcccggc accgatgacc gcaatacaca aagccaggtt
     7561 tactcggttg ataacctcaa ccacttcgcc gatgtgctca atgatattga tgaactggcg
     7621 cagttacagt taattcccgc cgacggtgcg gtcgccgaag cctcgccggg tcagtttgaa
     7681 atcaaccttt accataccga taacgtgctg gaagcctgcg atgatgcgct ggcgctaaaa
     7741 cgcctggtgc gtctgatggc agaaaagcat aagatgcacg ccacttttat ggcgaagccg
     7801 tatgaagagc acgcgggcag cgggatgcat atccatatca gtatgcaaaa taatcgtggc
     7861 gagaacgtgc tttctgacgc ggaaggcgaa gattcgccgc tgctgaaaaa aatgctcgcc
     7921 ggaatgatcg atctgatgcc gtcatcaatg gcgttgctgg caccaaacgt gaactcgtat
     7981 cgccgcttcc agccggggat gtatgtaccg acgcaggcgt cgtggggcca taataaccgt
     8041 accgtcgccc tgcgtattcc atgcggcgat cgtcataatc accgcgtgga atatcgcgtg
     8101 gcgggtgccg atgccaaccc atatctggtg atggcagcga tttttgccgg tattttgcat
     8161 ggccttgata acgagctgcc gttgcaggaa gaagtcgaag gtaacgggct ggaacaggaa
     8221 ggcttaccct tcccgattcg ccagagcgat gccctgggtg agtttatcga gaatgatcac
     8281 ctgcgccgct atttaggcga acggttttgc catgtctatc acgcctgtaa aaatgatgag
     8341 ctgttgcagt ttgagcgcct catcacagaa acagaaattg agtggatgtt gaaaaacgcg
     8401 taatacccct tgcggggttg tttcatcggc ctgagcggag ccaggcctga catttgctgg
     8461 taatccagac attttccgga tgtcgcgtta tggagcgcag gcggcaacgg gcttccgtta
     8521 acgattaaga ctgtgacgag gaaatgagat gatgccgatg attaaatccc cgcatggcga
     8581 gggaggctgc gtatgcgccc ctcctgcgac tgactggact ccgccccctt tgcttccctt
     8641 gctaaacagg tttgatttca ggtcgacacg accgcaaacc ttattacgca ggggaggcag
     8701 caattatggc tattaattca ccactgaata ttgctgcgca acccggcaaa acccgtctgc
     8761 gaaaatcact gaaattgtgg caggtggtaa tgatgggtct ggcctatctc acgccgatga
     8821 ctgtgttcga tacttttggc attgtgtcag gcattagcga cggtcacgtt ccggcgtcct
     8881 atttgctggc gctggcgggc gtgctgttta ccgctatcag ctacggcaaa ctggttcgcc
     8941 agttcccgga ggcaggttcg gcctatacct acgcgcaaaa gtcgattaac ccgcacgtcg
     9001 gatttatggt cggctggtca tcactgctgg attatctctt tttgccaatg atcaacgttt
     9061 tgttggcgaa aatctatctc tccgccctct tcccggaagt gccgccgtgg gtgtgggtag
     9121 taaccttcgt cgccatttta accgccgcga atctgaagag cgtcaacctg gtcgctaact
     9181 tcaataccct gtttgtactg gtgcaaatct ccatcatggt ggtgtttatc ttcctggtag
     9241 ttcagggact gcataaagga gaaggcgttg gcaccgtctg gtcacttcag ccgtttatca
     9301 gcgagaacgc gcacctgatc ccgattatta ccggggcgac gattgtctgt ttctcgttcc
     9361 tcggtttcga tgcggtgacc acgctttcgg aagagacgcc ggacgccgca cgcgtgatcc
     9421 cgaaagccat ctttctgacg gcggtctacg gtggcgttat ctttatcgcg gcgtcgttct
     9481 ttatgcagct gttctttccc gatatcagcc gctttaaaga tccggacgcc gcactgcctg
     9541 aaattgcgct gtacgtcggc ggcaagctct tccagtcgat tttcctctgc accacgtttg
     9601 tgaacacgtt agcgtctggc ctggcctcac atgccagcgt gtcgcgcctg ctgtatgtaa
     9661 tggggcgtga caatgtgttc ccagaacgtg tgtttggcta tgtacacccg aaatggcgta
     9721 ccccggctct gaacgtcatt atggtcggaa ttgtcgcgat gtcggcgctg ttcttcgatt
     9781 tagttaccgc cacagcgtta attaacttcg gtgctctggt ggcgtttacc ttcgtgaatc
     9841 tgtcggtgtt taatcatttc tggcggcgca aaggaatgaa taaaagctgg aaggatcact
     9901 tccactattt gctgatgccg ctggttggcg cgctgacggt gggtgtgctg tgggttaacc
     9961 tcgagtcaac gtcactgaca ctcggtctgg tatgggcttc gttaggcggc gcatatttgt
    10021 ggtatctgat ccgccgctat cgcaaagtgc cgctgtacga aggtgacaga acgccagtga
    10081 gcgaaacgta atagtcggat gcgcggcgca ttatcctgcc tgagggtaat gcgccgcgcc
    10141 attaactata aaaagatgta tgaccgggat cacgagtgct atcatccgcc cttatgctta
    10201 gcgcattaac cccaggggca aataatgaac cacgatatcc cgctaaaata ttttgatatt
    10261 gccgatgagt acgcgaccga atgcgcagag cctgtcgcag aggctgaacg cacaccgctg
    10321 gcccactact tccagttact gctcacccgt ttgatgaaca atgaagagat cagcgaagaa
    10381 gcccaacatg aaatggctgc tgaagcgggg attaatcccg tacgcattga tgagatcgca
    10441 gagtttttga atcaatgggg taatgagtaa ttatatcatt gcaggcgcct ggtcagtatc
    10501 gttgcccgga taaagcgttc acgccgcatc cggctaaaaa tgacgcacta cagcctgctt
    10561 taatgggttt ctcacgatcg taccgctccg ttcctcgcta atttgttatg tgagttatag
    10621 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    10681 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    10741 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    10801 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aacccacccc
    10861 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    10921 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    10981 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccagaacttg ccgaaagctg
    11041 ggaagtactc gacaacggcg cgacctatcg cttccaccta cgtcgcgatg ttccgtttca
    11101 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    11161 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    11221 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgtaaactgg ataatcatac
    11281 cgttgagttc cgtctggctc agccggatgc ttcttttttg tggcacctcg caacccatta
    11341 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggaaca
    11401 actcgaccgt caaccggtcg gcactggacc gtatcagttg tcggaatacc gcgccgggca
    11461 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    11521 ggtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    11581 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    11641 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    11701 gccgctaaat aatcccgctg tccgccatgc gctggcactg gcgattaata accagcgcct
    11761 aatgcaatcc atctattatg gtacggctga aacagcggcc tctattttac cgcgcgcctc
    11821 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    11881 actgaaagcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    11941 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    12001 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    12061 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    12121 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    12181 cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcggcgcg
    12241 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattgccca ttttgccgct
    12301 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    12361 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    12421 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    12481 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    12541 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    12601 attaacggtc aaccaattgc cgaacagtta aaagaggtct tcccggcgac gatggaactg
    12661 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    12721 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    12781 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    12841 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    12901 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    12961 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    13021 ctgatgcgta tcagcactat cgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    13081 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    13141 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    13201 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    13261 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    13321 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    13381 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    13441 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcggggctgg ctgtactgtg
    13501 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttaccaatt
    13561 attgccgccg tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    13621 cggacgcgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    13681 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    13741 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    13801 cctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    13861 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    13921 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    13981 tctctggttt gctgtgatgc ctaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    14041 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    14101 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    14161 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgctgttgg ttaacctcct
    14221 tggcgacggt gtccgtcgtg caattattgc gggggtggaa taatgccatt actcgatatt
    14281 cgtaacctga ctattgaatt taaaaccggc gatgagtggg ttaaagccgt tgaccgggta
    14341 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    14401 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    14461 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaagctg
    14521 gttggtcaca acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    14581 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    14641 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt gggaattaaa
    14701 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    14761 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    14821 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    14881 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    14941 gataaaatta acgtgcttta ctgcggtcaa acggtggaaa ccgcgccgag taaggagtta
    15001 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    15061 agcgcgatgc cgcataaaag tcgcctcaat acgctacccg gcgctatccc actgcttgaa
    15121 cagttaccga ttggttgtcg tctggggcca cgttgcccat atgcacaacg agaatgcatt
    15181 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    15241 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    15301 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    15361 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    15421 gctggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    15481 tcatccactg cattttggcg attattcctt ccgcagtcag cgtattcgca tgatttttca
    15541 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    15601 gcgcctgaac accaacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    15661 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cccccgggca
    15721 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    15781 tgaagccctc gcttcactgg atatgtcgat gcgttcgcag ttgattaatc tgatgctgga
    15841 attacaggaa aaacagggta tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    15901 gcacattagc gaccaggtgc tggtgatgca tcagggagag gttgtcgagc gaggcagcac
    15961 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccggtcattt
    16021 tggcgaggca ttgacagcgg atgcgtggcg taaggaccgt tagtgttctg cacctcatcc
    16081 tgacaggggc cccccttcag gatgaggtat tcattacttt tgattgtcga ttctggtgag
    16141 agcaaccagg aatgccagtg tataacacac cgtaattgcc agactcattt ttaacagtgt
    16201 ttctccgcca aatcccgaca atggcgcggc tataccgccg aagacaaaca tgctcatccc
    16261 catcaacgca gacgccgttc cagattcctg cgtccctact gcactcatag cctctgcccc
    16321 gccaacagtg ccgatgccgc tacaaaatgc aatagtgaaa aataatgcca ccagtgccgg
    16381 aatgggcaat tgtgtccatg cgcataagac cgtgagcaat gcacacaaaa ttgccgctat
    16441 caggccacct cgcaacaatg tcatcgcatt aatccgtcgc gccagacgcg aaaagatcca
    16501 tgaagcaatg ataagtccga tgccgttaag gccaaacacc aggctaaatt gcattggact
    16561 aaagccaaat tccttctgca acacgaacga cgaagagccg atatacgcaa acaagcctgc
    16621 cagcataaaa ctttgaatca ggcaaaagcg cataaaacgg cggttctgca ccacgcttcg
    16681 tccggttaat agcaatgatg agcccctttt attttctggc aaggtctcat taataaataa
    16741 catgcagccc agcaacaata cggtgctaat ttcagccatt acccagaata aagtacgcca
    16801 gtcaaaagtg ctaacaatgt acccgcccag cactggcgac aacaccggtg ccaggccatt
    16861 caccgtcatt aacagcgcaa aaaactgggt caacgttact ccctgatatt tgtcacgagc
    16921 aatagagcga gagagcaccg aaccacccgc ccccgcgatc ccttgaataa aacgccagac
    16981 caccagccag taaatattgt tcgttgtcgc gcacaaaatg gaagaaacaa taaacaatag
    17041 caacgacaag attaacggac gtttacgccc tattttgtcg ctcagagggc caaataacag
    17101 ttgtccgacg ccaagaccga tcagtgatgc agtcagcgtt aattgcgtta tggtcgtggt
    17161 tgccgccagt tgctcagaca tctccggcaa agccggcaaa taaagatcgg tacacatcgg
    17221 gccgataccg gctaaaagac caagaatcaa tgcccatgaa agagagactc tagccatatt
    17281 attccttact tgagtgatga catgccgccg cccagcgact gccagagcgt gattcggttt
    17341 gtaaaatcag tcagttgcag tgccagcagc tcctgctgtg cgctccacaa gctgcgctgc
    17401 gccgtcaaca cggtcaggta atcaccaaca cccgtctgat agcgacgcaa gccaacatcg
    17461 accgtttgtt gctcagcttt aacatactgg cgctgtgcat ccagttgctc ttcaagcgtg
    17521 gtccgccgcg ccagtgcgtt agcgacatct ttaaatgcgc tctgaacggt tttttcatag
    17581 gtggcaatga gtccacgctt ttgtgcttct gcgtaacgaa gctgcgccag attacttccg
    17641 ccagtgaaca gcggtaatgt gaccgatggc gtaaatgacc agatctgcat tccgtggctg
    17701 aacaacgacg acaatgcgtc actgccaaca ccagcgctgg cagtcagcga aatcgtcggg
    17761 aaaaagttag cccgcgccgc gccaatatcg gcattggcgc tcttcaaatt atgttccgct
    17821 tcctggatat ccgggcgacg cagcagaacg tcagatgaca ctcccgcagg aaccagactt
    17881 atcatctgtt ccggcaggct ttccagtgtt cccggtagca aattttcctc aagcgttgta
    17941 cctgccagta agttcagcgc atttttatcc tgcatcactt gtgtctgata gctggcaacg
    18001 ctggcgcgcg cttgctgata aacactcatc gcctcactga catctgtcgc tgccgccgtg
    18061 ccaacctgtt gctggcgctg gataattttc aatgagtttt ctgcactggt catcgtttct
    18121 tttgccaggg ccaggttgct gttatcggcc gccagtgtca gccaggcggt gctgatttca
    18181 gcaatcagcg ttaaacgggt gttctgggct gtaaattcac tggcaagcca ggtttctcgc
    18241 gccgcccgag ataaactctg attacgacca aacagatcta acgtaaaact ggacaccgtg
    18301 ccgtcagcct cggcagtggt ttccgtacca ttcgccagcg aacgactgcg ggttgaactt
    18361 aaggccgcat tcaccgtcgg gaataacgac gcattagttt gcccatagag cgcccgtgct
    18421 gagtcaatat cagcaatcgc tttttgcaca tcgcggttac tgttcagtgc aatagtcacc
    18481 acctgctgca atcggtgatc gtggatcacc tgctgccagt catggctgat tgcctttccc
    18541 tggccctgtg ctcccggcaa ggtcgccggg attggggatt caggcgtgct gtagtgggga
    18601 tcgagcgaga cacatcccgc actcaacagc accagaaaaa taagagaacg acgcaacata
    18661 acttactcct gacggcgcgg tttaccggca aacaaacgtt tcaccagtac aaaaaacaga
    18721 ggaacaaaga aaatagccag caacgtagcg gtcagcgtac cgccaataat gcccgtacca
    18781 atggcgatgc ggctgttcgc ccctgcgccg gttgctatcg ccagcggcat tacccccgca
    18841 ataaacgcca gcgaggtcat gatgattggg cgtaaacgag tctgcgccgc gcgtaatgcc
    18901 gcacgactca aggaatagcc ctctgcaacc gccgcttcag caaattccac aatcagaatg
    18961 gcgtttttcg acgacaagcc gatagtggtt aatagcgcca cctggaagta aacatcgttg
    19021 tttaaatcac gcatccaggc cgccagcgcc gcgccaagaa gccccagagg gatcaccaga
    19081 atgaccgaga acgggactga ccagctttcg tacagtgctg caaggcagag gaaaaccacc
    19141 agaatagaaa ctgcatacag actcaatgcc tgaccactgg ccagtttctc ctgcaatgac
    19201 aaaccactcc aggcccaggt tgttcccgca ggaagactgt ttgccagttc ctccattttt
    19261 gtcattgcat cgccggaact aaagccagtc gcattttccc cctgaatttc ataggctgca
    19321 gaaccgttgt agcgcaccag tctttccggt ccatacagcc agcgggtggt cgcaaaagca
    19381 gagaatggtg tcatggcgtt atcactaccg cgcacaaacc atttacctaa gtctgacggc
    19441 gcggaacgaa attcactgtc gccctgaatg tagacttttt tcacacgacc acggtcaata
    19501 aagtcattca cataagtacc gccccacgca ctggacaggg tgtcggtgac atcattcaaa
    19561 ctcaacccta atgacaccgc tttattacta tcaatatcta cctgcaattg cggcatttgt
    19621 ggcaaatcat tggcacgaac agaatgtaac tccggacttt gattcgcttt ttctatcaac
    19681 tgattacgca tttgcagtag tgtttcgcga tcggttccac cgttagctaa caattcaaac
    19741 gtaaaaccat tgctttgccc cagcccatca acggctggcg gcgtcatcgc aaataccgtg
    19801 gcatcacgaa ttgtgcccag ctctttggtt gcccgtaggg cgatagcctg ggcggtgttt
    19861 tctgcccctt tacgttgaga ccagtttttc aacgaaacaa acgccatccc ggtgttctgt
    19921 ccgctgccgc taaaactgaa accatcaacg gtaaaaatga catcggtatt tgctttctcg
    19981 ttaatcagga accagtcaac aatctggcga ttcacttctg ctgtacgggc agcggtcgcc
    20041 ccggccggca gcgtgtactg caccatgatt tcgccctggt cttcagtggg taagaaactg
    20101 cccggcagtt tccacatcat cagggccatt ccaccaccca gtacgagata aagccccatc
    20161 gttcgagcag cacggcgcag gacataaatt acgcctcgct gatatttatc ttcagtacgg
    20221 cggtaaaagc ggttaaatgc gccgaaaaag ccttttttat gtggcggaac atgctggagg
    20281 acggaaccgc acagggcggg agtcaaggtc aatgccacca ccacggaaag cagcattgcg
    20341 gagataatgg ttatcgaaaa ctgacgataa attacccccg tagatccccc aaagaaggcc
    20401 ataggcagga atacggctga caacaccagc gcaatggcaa ccagtgcgcc agaaatctcg
    20461 cccattgatt tttccgtggc ttcacgcgcg ggcaaccctt cgtcacgcat aatgcgttcg
    20521 acgttctcca ctaccacaat ggcatcgtcc accagcaagc cgatcgccag caccattgca
    20581 aacaacgtca gggtattaat cgaatagcca aacaacgcaa gaacgccgaa tgtgcccagt
    20641 aaaacgacgg ggacagctaa cgccggaatc agtgtggcac gcaggttttg caggaataaa
    20701 tacatcacgc aaaccaccaa tacgatggct tcaaacagcg tctgaattac gtcctctaca
    20761 gagattttaa taaattcagt gctgtctttc gggtaagcaa tgtcgtatcc ctgcggcatg
    20821 ttccgctgga attcggcaat cttatccttg accagcgtcg ccgtattcag cgcattcgca
    20881 ccgggcgaaa gcattacagc aaccccggca gacgggtgac cgttaagttt gccgatagcg
    20941 gtataatctt cactgcccat ctcaacgcga gccacatctt ttatacgaac aactgcgccg
    21001 tctgactggc ttttcacgat aatatttttg aactgatcca ccgtctgcaa gcgagactgc
    21061 gcacgtaccg ttgcggtcag ttgctgagta ttcggtgaag gcaatgcccc tattttcccg
    21121 gcagtaacct gcacgttttg cgcttcaata gcactttgca cgtctgaagg cattagcgag
    21181 taagacgcca gtttggccgg gtcaagccag atgcgcatag cgtattccgc gccaaagact
    21241 tgcagactcc ccacaccttc aacacgcgcc agcgggtcct gaacgttact aaccaaccag
    21301 tcggcgatat ccgaactgga ggctttgtca gtggtgtcat acacggcagc aatcagcaaa
    21361 aagttgcttt gtgatttctc caccgtaaca cccgtttgct gcacttcgct gggtaggcgc
    21421 gactccgcct gctgaatttt attctgcacc tgaacctgtg cagtatctgg atcggtacct
    21481 tgttcaaagg tcacattaat actgaccgaa ccatcagagc tgctggttga gctgaaatag
    21541 agtaaattat caagcccagt gagttgttgc tcgataacct gagtcacgct gttctccagc
    21601 gtttcggcag aagcaccagt ataagtggct gaaattttaa tggtcggtgg cgcaacgtca
    21661 ggatattgcg cgacgggcaa tgtgcgaata gccagaatcc ccgccaacat aatcaaaatg
    21721 gcgataaccc aggcaaagac cgggcggcga acaaaaaagc gcgaaaacat caggcgttgc
    21781 ctccattagc ctgaacttca acagccttga ccgtctgccc tgaagtgact tttgcagaac
    21841 cttcaacaat cagtcggtcg ccgttgtgca ggccgtttag caccagccat ttatcaccat
    21901 atgtttctcc cgtttcgagc gttcgctgct ctactttatt gtctttattc accaccagcg
    21961 cagttgcatt gcctttagca tcgcgcgtga cgccctgttg cggcgcgaga atagcgtctt
    22021 ccatcacgcc ttcatcgaca cgagcgcgaa caaacattcc cggtaataac tgctgttgtg
    22081 gattggggaa aatcgcccgt aatgtcaccg aaccggtaga ctcatctaca gcgacttcgg
    22141 tgagttccag acgccctttt tcgctgtagg ttgtgccatc ttccagaata agtgagacgc
    22201 tcatggtgtc actgttggtc gccagtgact gtttacgcag acgtaataaa tcgacactgg
    22261 agcgagtgag gtcgacatac attgtatcca gaccacgaat agtcgttaac gctgtatctt
    22321 gcgacgcggt caccagtgcg ccaggggtta ccgacgaaat gccaatgcgc cctgaaatcg
    22381 gtgcggttac cgtggtccag tcaagattaa tgcgcgcagt ttctagtgcg gcttttttcg
    22441 cctctacact ggctttatct tgtgcacagg tagactgagc atcatcagca tcctgttgtg
    22501 aaacaccgtt ctctttcacc agtcgggcat aacgctgcgc tttctggcaa tcggctttta
    22561 ccagtgcctg tgcttgttgt aatgctgccc gagcttcatt ccatgcagcc tgataactgg
    22621 ccgcatcaat ctggtagagc ggctgtccag ccttgaccag atcaccttcc ttaaataagc
    22681 gtttctggat aattccccca acctgcggac gtacttcggc actgagcgca gcactggtgc
    22741 gtccggttaa ttcactgact accgagaccg gttgagattt gactgtaaca acgccgactt
    22801 ctgtttctga cggggatgaa ttgttacttt gcgtattatc acaaccagat aagagcagca
    22861 ttgccactac agatgttgct atatacttca ttattttctc cagggtgaga aataccctcc
    22921 tcattaaaat taatgaggat atgtcaggga tattcagtta taacgaaccc gcattgcgaa
    22981 atgaatttcg caatgaaata agggggatta atatcccccg ctattggtta attctgttct
    23041 tatttattta tcaacatact aacaatatcc tgatatattt cctgcagatg ttgtggatcg
    23101 cttttttgcg gtgttaaacg acgataaacc gtaccttcca tcatggtagc aaacacttca
    23161 acgcagcaac gaatgtgctc gtcgctaaga tggggatgca ttcttttcat atgtgcacaa
    23221 ccgttagcga acattctggc gtcagcttct tcaagcattg ccataacccg cgggttgcgt
    23281 gtggcttctg cagccacttc cagcatcagc gcgtcgtcgt cctcatttaa ggatttacgc
    23341 aaggcaagga cttccgggaa gtgatcggta cgcgcgtcaa tgtccatttg ggcaatacga
    23401 aagtcgatga tacggcgaac catctcttca ataatggcgt ctttgttggc gaagtaacga
    23461 tatatctgac ccacgctaag ctgagcttcg ctggcaattt gcgacatact ggcggcatga
    23521 aacccgcagc gacgaaaaca ccgacgggcg gcgttaatga tttcatcctg acgctgtttg
    23581 tggcgtatct ccagcttgct ggtcatttct gtctcctggc ctatcacttg gtgagaatga
    23641 tcgttctcat ttttgctgat tatacgtttc tccaaagtaa tggaaagtat ttttatctta
    23701 ttcatggtgc atattctgat aatgaagaga ttgtggagat tctgtgaaga tagttgacaa
    23761 tattaagttg gggtcaattc atcagaaagg cagctacttg ttgtcgccag ttctgttttc
    23821 atcatcaaac ttatcggagc taatacgaat agtttcatag tcagattcta taagatattt
    23881 ccccaatcta ccccttgcag gcgtcaagag tagataacgc aaaatgctct gtaccgcagc
    23941 ttctctccgg tacagaaagc gcaactatag ccacccacag ccaggttgat tataataacc
    24001 gtttatctgt tcgtactgtt tactaaaacg acgaatcgcc tgattttcag gcacaacaag
    24061 catcaacaat aaggattaaa gctatgggtt ttctttccgg taagcgcatt ctggtaaccg
    24121 gtgttgccag caaactgtcc atcgcctacg gtatcgctca ggcgatgcac cgcgaaggag
    24181 ctgaactggc attcacctac cagaacgaca aactgaaagg ccgcgtagaa gaatttgccg
    24241 ctcaattggg ttccaatatc gttctgcaat gcgatgttgc cgaagatgcc agcatcgaca
    24301 ccatgttcgc tgaactgggg aaagtttggc cgaagtttga cggtttcgta cactctattg
    24361 gttttgcacc tggcgatcag ctggatggtg actatgttaa cgccgttact cgtgaaggct
    24421 tcaaaattgc ccacgacatc agctcctaca gcttcgttgc aatggcaaaa gcttgccgct
    24481 ccatgctgaa tccgggttct gccctgctga ccctttccta ccttggcgct gagcgcgcta
    24541 tcccgaacta caacgttatg ggtctggcaa aagcgtctct ggaagcgaac gtgcgctata
    24601 tggcgaacgc gatgggtccg gaaggtgtgc gtgttaacgc catctctgct ggtccgatcc
    24661 gtactctggc ggcctccggt atcaaagact tccgcaaaat gctggctcat tgcgaagccg
    24721 ttaccccgat tcgccgtacc gttactattg aagatgtggg taactctgcg gcattcctgt
    24781 gctccgatct ctctgccggt atctccggtg aagtggtcca cgttgacggc ggtttcagca
    24841 ttgctgcaat gaacgaactc gaactgaaat aatcgttctg ttggtaaaga tgggcggcgt
    24901 tctgccgccc gttatctctg ttataccttt ctgatatttg ttatcgccga tccgtctttc
    24961 tccccttccc gccttgcgtc aggataacga tttcctttac gaccaaggag cgcccatgga
    25021 acaacgccac atcaccggca aaagccactg gtatcatgaa acgcaatcca gtactacgga
    25081 gtatgacgtt ctgcctctgg tcccggaagc cgcaaaggtc agcgatccct ttctactcga
    25141 cgtgatcctt gaaaaagaaa cgctggcccc cttcctttca tggctggacc ctgcgcgtgt
    25201 tcttgcagtg gatttgttcc ctgaccagct taccgtgacc cgttcacaga ccttcaccgc
    25261 ttatgaacgc ttgtcgacgg ccctgacggt tgctcaggtt tgcggcgtcc agcggttatg
    25321 taactactat tcggcgcgac ttacgccgct ccccgggcct gattccacca gggaaagtaa
    25381 tcatcggttg gcacaaatca cgcaatatgc ccgccaactg gctagctcgc cttctattat
    25441 cgacaaccga tcgcgccagc atctgaatga cgtcggtctt actgcctggg actgtgtgat
    25501 cattaaccaa atcattggtt ttattggctt tcaggcgcgg acaattgcga catttcaggc
    25561 ttatctcggg catccggtac gctggttacc cgggctggag atacaaaact acgccgacgc
    25621 gtcactgttt gctgatgaat cattacgctg gcgaagcagc tatgaggtgg aaaaactacc
    25681 tgaagaacac acaaaaagtt caactgcaga actttgccaa ctggccgaaa tactctctct
    25741 ccaccctatt tcactttccc ttctcgaaag gttgttaaac agcacacggg ttaatacaca
    25801 gccggataat cagcttgcgg cgttgttatg cgcgcggata aatggcagtc ctgcttgttt
    25861 tgccgcctgt atggattcat caaatgaata taaaaaaatc agcacccttc tgcgcaaggg
    25921 cgaaaatgaa attaaccaat gggctgaccg tcattctgtt gagcgcgcta ccgttcaggc
    25981 gatacaatgg ctgacccgag cacccgatcg ctttagcgcc gcccagttca gccctttact
    26041 cgaacacgaa aaatcatcaa cgcagattat taatctgctg gtatggagcg ggctgtgtgg
    26101 ctggataaat cgcttaaaaa tcgcgttggg tgagacatat taaccttgcc gcgtcagaca
    26161 gattcgcgta aaactgtcag ccgctctaat ggccaccaaa atagacaatt atgtttcagg
    26221 acaacccgct gctagcgcag cttaaacagc aactgcattc ccagacgcca cgcgctgaag
    26281 gggtggtaaa agccacagaa aaaggttttg gcttcctgga agtcgacgcg caaaaaagtt
    26341 atttcattcc gccgccgcag atgaaaaaag tcatgcatgg cgaccgaatt atcgcggtga
    26401 tccacagtga aaaagaacgt gaatccgcag agccagaaga actggttgaa ccgtttctga
    26461 ctcgtttcgt gggtaaagtt cagggcaaaa atgaccgtct ggccatcgtt cctgatcatc
    26521 cactcttaaa agacgccatt ccttgccgcg cagcccgtgg tctgaaccac gagtttaaag
    26581 aaggcgactg ggcagttgcc gaaatgcgcc gtcatccgct gaaaggcgat cgttctttct
    26641 atgctgaact gacacaatac atcacttttg gtgacgacca ctttgtaccg tggtgggtta
    26701 cccttgcgcg ccataatctg gaaaaagaag caccagacgg cgtcgctacc gaaatgctcg
    26761 atgaaggtct ggttcgtaaa gatctgaccg cgctggattt tgtcaccatc gacagtgcca
    26821 gcacagaaga tatggatgac gcccttttcg ctaaggcgtt gccggatgac aaacttcagc
    26881 tgattgtggc gattgccgat ccaaccgcgt ggattgctga aggcagcaag ctggacaaag
    26941 ccgcgaaaat tcgcgcattc accaactatc tgcctggctt caacatccct atgctgcctc
    27001 gcgagctttc tgacgatctc tgctcactgc gcgccaatga agtccgccca gtactggcat
    27061 gccgcatgac gctctccgct gatggcacca ttgaagataa tatcgaattc tttgccgcca
    27121 ccatcgaatc caaagcgaag ctggtgtatg accaggtttc tggctggctg gaaaataccg
    27181 gtgactggca gcctgaaagt gaagcaattg ccgaacaagt ccgtttgcta gcgcaaattt
    27241 gccaacgccg cggcgagtgg cgtcataacc acgcactggt gtttaaagat cgcccggatt
    27301 accgctttat tctcggtgaa aaaggtgaag tgctggatat cgtcgccgag cctcgtcgca
    27361 ttgccaaccg tatcgtcgaa gaagcgatga ttgccgctaa catttgtgcg gctcgcgtac
    27421 tgcgcgataa gctcggtttt ggtatctata acgtgcatat gggctttgat ccggcgaatg
    27481 ccgacgcgct ggcagcgttg ctgaaaacgc acggtctgca tgtcgatgcc gaagaagtgc
    27541 tcacgctgga cggtttctgc aaactgcgtc gtgaactgga tgcgcaacca actggtttcc
    27601 tcgacagccg cattcgtcgc ttccagtcat ttgctgaaat tagcactgaa cccggtcctc
    27661 actttggcct cggtctggaa gcatacgcca cctggacttc gccgatccgt aaatatggcg
    27721 acatgatcaa ccaccgtctg ctgaaagcgg ttatcaaagg cgaaactgcg acgcgtccac
    27781 aggatgaaat cactgtgcaa atggccgagc gtcgccgtct caaccggatg gcagaacgtg
    27841 atgttggtga ctggttatac gcacgcttcc tgaaagacaa agccgggacc gacacccgtt
    27901 tctcggcgga aattgtcgat atcagccgtg gcggcatgcg tgttcgtttg gttgataacg
    27961 gcgctatcgc ctttattccg gcacctttct tacacgctgt gcgcgatgaa ctggtttgca
    28021 gccaggaaaa cggcactgta caaattaaag gtgaaacggt ttacaaagta actgacgtta
    28081 ttgacgtcac cattgccgaa gtccgcatgg aaacccgcag cattattgcg cgcccggtcg
    28141 cgtaatctcc tttcacggcc cattcctcat ggatgggccg tttatttccc cgctctacct
    28201 tcgttatatc caggcagatt ttttaactga ctttcgtttg aaaactggcg tttttccaaa
    28261 actggattac aaataaatat ataaaaaaaa caatgcgttt agccgccgtt aatttcatgt
    28321 aagtcatgtt gttcctttta tttaaccgcg gactccgcag ttaaccggag gatatgcatc
    28381 atgaaaaccg ttagggagtc cacaacgttg tacaactttc tcggatcgca caatccatac
    28441 tggcggttga cggaaagcag cgatgttttg cgcttttcta ccaccgaaac cacagaacct
    28501 gaacgtacgt tgcagttatc tgccgaacag gctgctcgca tcagggaaat gacggtcatc
    28561 acctccagcc tgatgatgag tctgaccgtc gatgagagcg atctttctgt gcatctggta
    28621 ggacgaaaaa tcaataaacg ggaatgggct ggcaacgcgt ctgcatggca tgacacacca
    28681 gcagttgctc gtgatttatc acacggactt tcctttgctg agcaggtagt ttctgaagca
    28741 cattccgcaa tagtgattct cgacagccgg gggaatatcc aacgcttcaa tcggttatgt
    28801 gaagattaca cagggttgaa cgaacacgac gtcattggcc aaagcgtgtt taaactgttt
    28861 atgagccgtc gtgaagctgc ggcatccagg cgcaataacc gtgtattttt tcgaagcggc
    28921 aatgcatatg aagtcgaact gtggatacca acacgtaaag gccagcggct gtttctgttt
    28981 cgcaataaat ttgtccacag cggcagtggc aaaaacgaga tttttttaat ctgttccggc
    29041 accgacatta ccgaagagcg ccgcgctcag gagcgactgc gtattctggc aaataccgac
    29101 agtatcaccg gactgccgaa tcgtaacgca atgcaggagt taatcgatca cgctattaat
    29161 caggcagata acaataaagt tggggtcgtg tatcttgatt tggataattt taaaaaggtc
    29221 aacgacgcct atgggcattt gtttggtgac cagttattac gcgacgtgtc attggctatt
    29281 ttaagctgtc tcgaacatga ccaggtgttg gcgcgtccag gtggcgatga gtttctggta
    29341 ctggcatcca acacctcaca aagcgcgctg gaagcaatgg catcacgaat tttgacccgc
    29401 ttacggctcc cctttcgcat tggtttaatt gaagtttata ccagctgttc agtaggtatt
    29461 gcactctctc ccgaacatgg ttcggacagc gcggctatta ttcgtcacgc cgacacagca
    29521 atgtacacag caaaggaagg cggacgagga caattttgcg tttttacccc agaaatgaat
    29581 caacgggtat ttgaatatct ctggctggat accaacttgc gtaaagcact ggaaaacgat
    29641 cagttggtta ttcactatca accgaaaatc acctggcgtg gcgaagtgcg cagtctggaa
    29701 gcactagtac gttggcagtc acctgaacgt gggttgattc caccgttgga cttcatttcc
    29761 tacgcagaag agtcagggct aattgtgcct ttaggccgtt gggtgattct cgatgtcgta
    29821 cgccaggtgg cgaagtggcg ggataaaggc attaacctgc gagtggcggt aaacatttct
    29881 gcacgtcagc tcgccgatca aaccattttc accgccctga aacaggttct ccaggaactc
    29941 aattttgaat actgccctat tgatgttgaa ttgacagaga gttgtctgat tgagaatgat
    30001 gaactggcac tgtctgttat tcaacaattt agccaactag gtgcgcaagt gcatctggat
    30061 gattttggta ccggctactc ttcactttcg caactggcgc gctttccgat cgatgccatc
    30121 aaacttgacc aggtttttgt tcgagatatt cacaaacaac ctgtctcgca gtcactggtt
    30181 cgggcaatcg tcgctgtggc ccaggcattg aatcttcagg tgatcgccga aggtgtagag
    30241 agtgcaaagg aagatgcttt tttaaccaag aatgggatca atgagcggca aggatttttg
    30301 tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt ataaacgcta tctgaagcgc
    30361 gcataagtag atgaatatat atacttagcg ctatattccg tcattatata tcgtattccc
    30421 ggatgataat tagccgttgc tgttaaacac ttctctttta gcggcaattt ttgcattatt
    30481 cattttcccc cttttgctat aaggatgtcc ttgatgtctg aatttgatgc ccagcgcgtt
    30541 gccgaacgta ttgatattgt gttagatatc cttgtggcag gcgattacca ctctgcgatc
    30601 cataatctcg agattcttaa agcagaactc ttgcgccagg ttgctgaatc tacgccagac
    30661 atcccgaagg caccgtggga gatttaacaa tcgtgccttc aggttttcct atttgaggat
    30721 gcccggcgcg tcagtatttt ttattaagta ttataacgtt ataagaatta cagcgatgaa
    30781 ttcccgacaa caaactattc tacagatggt cattgaccag ggtcaggtta gcgtaaccga
    30841 tctggcaaaa gccactggag tttctgaagt taccattcgc caggatctca acaccctcga
    30901 aaaactgagt tacctccgcc gtgcacatgg ctttgcagtt tcgcttgata gtgatgacgt
    30961 cgaaacccgt atgatgagca actatacgct gaagcgtgaa ctcgccgagt ttgccgcgtc
    31021 actggttcaa ccgggcgaaa ccatctttat cgaaaatggc agcagcaatg ccctgctcgc
    31081 ccggactctg ggggagcaga agaaaaatgt cactatcatc acggtcagca gctacatcgc
    31141 gcatttgctg aaagacgcgc cttgtgaagt tattttgctc ggtggcgtgt accagaaaaa
    31201 aagcgaaagt atggttggcc cgttgacacg ccagtgcatc caacaggtac atttcagcaa
    31261 agcatttatt ggtattgatg gctggcaacc tgaaactgga ttcaccggtc gcgatatgat
    31321 gcgtaccgat gtggtgaatg ccgtgctgga aaaagagtgc gaagcgatag tcctgactga
    31381 cagctcgaaa tttggtgctg tacattcata ctccatcggt cccgttgagc gattcaatcg
    31441 cgtgattacc gattcgaaaa tacgcgccag cgatctgatg catcttgagc acagtaaact
    31501 caccgttcac gtcgttgaca tttaaccatt tcccttctct acggatgatt tgcagtttgg
    31561 caaatcatcc gctctaagat gattcctggt tgataattaa gactatttac ctgttattaa
    31621 cactcttaag atataaaatt attatcagcg atataacagg aagtcattat cacctgcgtg
    31681 atataaccct gcgcgcgagc ggatttcacg gaataatttc acccagctta ttcttagcta
    31741 ttatagttat agagagctta cttccgtgaa tcataaattc aggagagagt attatgtttg
    31801 taacgagcaa aaaaatgacc gcggctgttc tggcaattac cctggcaatg tctctgagtg
    31861 cctgttctaa ctggtctaaa cgggaccgca acaccgcaat cggcgcgggt gcaggggcat
    31921 taggcggtgc agtactgacc gatggcagta cgttgggtac attaggtggc gcagctgtcg
    31981 gtggtgttat tggtcaccag gtcggtaaat aatcgtaaat aaatgtcgat tatcgactcg
    32041 caattataat acgcttgatt ctgttcagta cgaaataact gaataataca cacagtaaaa
    32101 tattcgaaag ccacggatat atccgtggct ttttatgtta accgcctgcg agttttacct
    32161 tcatcccttt cgcttcaagc agtgatttta atagatcgcg cttatcgccc tggatttcaa
    32221 taactccatc tttaactgct ccgccgcagc cgcatttttt cttcagttcc gctgcaagtt
    32281 ttgtcagttc ggcatcatcg agatcgatac cggtaatcag gcaaacgccc ttacctttac
    32341 gtccactggt ctgacgctga atacgcacca caccatcgcc tttagggcgt actggggccg
    32401 ctttgggctc atcaatacgc ccggtttctg ttgagtagac cagacggctg ttggaatcac
    32461 tcatcatgca ctccgctgta aagaggcgtt gatcgctttc agcgtctgcg ctggatctac
    32521 cgattgcgtt accgggcgac caatcaccat ataatcaaca ccagccgcca acgcctgttc
    32581 tggcgtcata atgcggcgct ggtcacctgc ttcactcccc tgcggacgaa tgcccggtgt
    32641 aaccagtttg aactcctgac cgaatacctg tttaaagcgc acagcttcct gagcagaaca
    32701 caccacacca tcaaggccac atttttgcgt cagtgccgcc agacgttctg cataatctgc
    32761 aggtgacagt gtcacgccaa gatcggccag gtcgctggct tccatgctgg tcaacactgt
    32821 cacagcaatc aaaagcggtg catctttgcc atacggaacc agtgcctcac gcgctgcggt
    32881 catcatacgc gccccaccaa aggcatgaac attcaccatc cacacgccta agtcagctgc
    32941 agcagcgaca gcgtgcgctg cagtgttggg gatatcgtgg aatttcaggt caagaaagat
    33001 atcaaaacca cgctgttgaa gttcgcgcac aaactgtggc ccaaacaatg taaacatctc
    33061 tttgccgacc ttcagacgac aatcgcgtgg gtcgatcttg tcgacaaagg ccagcgcggc
    33121 atcacgatta tgataatcaa gggcaacaac cacaggagaa ttcgtaacag cgcgggaaga
    33181 agatgaagca gttaacgtca tgaccagacc ttcttgatga tgggcaccgg aagtggcgcg
    33241 aaacaggtaa acggcgagca ttctacccgt ggaaataaaa aattaacagt tgcgatttct
    33301 cccctggcag gctttttctg cgccgtcgtt gccggagcac aatgggacat caataactag
    33361 tatgttgtaa ctaaagtcgg tttttttaaa aaattacagg ccatcaagac cgcgaatcgg
    33421 tttaatagtt gaccaggcac gacaagacgg acaatgccag tagagggtgt atgcggtaaa
    33481 accacatttc tggcagcgat aacgaggctt actacgtacc ttctcgccaa ccatgtcacg
    33541 cagcaccatc agactctctt tggcacgccc ttcttccgct tcatttaagt ggtaatccat
    33601 tagcttatgg aacacacgca tggtcggatg acgctgaagc tggcgcgtaa tatagacctg
    33661 tgcggcctca ctaccgtcgc gcgcttcgat gatatccgca agcatcaatt cagcatcggc
    33721 accggtgttc tcttccaccg cgcgctgcag gaattctgcc cattcggcag ttttacccaa
    33781 ctgctgatag caaatttgca acatttccag cgtttcgcta accagttctc tgtcctggga
    33841 tatgacacgt tgcagacttt cgacggcttt ggcgtattct cctttcgcca taaacacgcg
    33901 tcccatcatg atggatacgc gggcgctgtt tttatctgcc gccgcccctt tttttagcaa
    33961 ggccatggca cgatcaagat cgtcgctggc catatgctgc agggctaact cacagtagaa
    34021 atgggcaatt tcgacgcgct gtttatcttt acccagcttc accaggcgtt cggcaacatc
    34081 aattgctttc tgccactcgc tggtagcctg gtagatttgt agcaactgtt gcagcgcgcc
    34141 aatgcggaag tcagtttcat cggtcagctg attgaacatg tcttccgcgc ggtcatataa
    34201 cccggcggcc atgtaatcac gccccagttg ttgaatcgcc aacagacgct gttcataggt
    34261 cagcgaggcg ctttccatta gggtctgatg gatgcgaata gcgcgatcaa cttcgccacg
    34321 cgaacggaac aggtttccga gcgtaaggtg ggcttcaacg gtgcctgtat cctctttaag
    34381 catatcgaga aacaggtcta ccgctttatc ctgttgatta ctaagcagga agttaacccc
    34441 cgctacgtaa tcacgcgaca agcggttggc ttcatcttgc ttgttttgtt gcgcacttct
    34501 gcggcccata taccagccat aggcagcggc tacaggcaaa agcagaaaca acaactccag
    34561 catagaaagt tattccttcg ccgctgacga gtgcggcact acagccacgt cagtcgcggg
    34621 tgaaagctgg ttttccagtc gctttatttt acgttcagcg cgcgccaggg aaacacgaac
    34681 tcgcagccag aacaggccac aaatcaacca accgatagca aaccccgcag caaacaatac
    34741 cgccagcaat gtggaaatac ggtactcccc ttgcgctaac agataattaa acgtcacctg
    34801 ttgatcgttc tgcgcaccca acgtgaccga aatcacgaag atcgctaaca ccagtaagaa
    34861 aatgagtaaa tatttcacat tacttcccgt tatgtggtta gagcgaataa attatggtca
    34921 acttaccgca atagcccttt aaagtaccat tttcgcgccg tctgcgaaat ggcatccacg
    34981 cgcattgttg atgatttaag ggctaaattc ggaaaatcaa ccagcgttaa ctttcttgtt
    35041 ctcgttgcgc tatttcgcga ttttcttccg caggtggtgt taatggccca caaattcgtt
    35101 gcgcaagcca cgttgccacc gccaccagcg cccacgaaat caacgtagct actaccagat
    35161 cgcgtggcca atgcatcccg agcagcaggc ggcttcccat gactccagtt gcccagacca
    35221 gcaagatagc aatggttaac gttcgccgac gcggccacag caaaccaacg gccagcagtg
    35281 cccaactggc agcaaacatc gtgtgaccgg aaggaaaggc aaaccccgtc tctttctgcc
    35341 agtgtgaacg caaatattgt ggaatatttt tctcttcagc caactgttct ttcactaaat
    35401 ttccgcgttc tgctcgcttt aaagtgtaga actcatcaac cggaatatga tgtgtttttt
    35461 ccagccagat aacaaaaggt cgtggttcct ggactttgtc tttgatccag gatttaacgc
    35521 cttgtcccac aaggattgcc gccgccagaa tggcaaataa tacaaaggca gccttaatgc
    35581 gaaaacgcag acaccagaga aaccagccga ataaaatcaa atgtgtaatg acgccccagg
    35641 gctgggtgac agtttcagta acccaaaaag ccgcttttag tagccaactt tgttctccag
    35701 gttgccaacg ccagccagaa atccatacgg ctactggcat aacaagcaat agtgcagctc
    35761 ccactgcggt acgtcgggca atcgaacgca tggcctctcc tttttgataa gtcccacaat
    35821 cataactgaa aatgccagtt ccaggaaaaa ttgacagatt tgtgccattc cgtgaacgat
    35881 cgacgcgtcg tgattaggtg aaccccttct cgttatggca aaataagcca atacagaacc
    35941 agcattatct ggagaatttc atgcagctta aacgtgtggc agaagccaaa ctgccaaccc
    36001 catggggcga tttcctgatg gtgggatttg aagaactggc aaccggacac gatcatgtcg
    36061 cgctagtcta tggcgatatt tccgggcata ccccggtact tgcgcgcgtc cattccgaat
    36121 gtctgaccgg tgacgccctg ttcagcttgc gctgcgattg tggcttccag ctcgaagcgg
    36181 cattgacgca aattgccgag gaaggccgtg gtattttgct gtatcaccgt caggaaggtc
    36241 gtaacattgg tctgctgaat aaaatccgcg cttacgcact gcaggatcaa ggttacgata
    36301 ctgtagaggc taaccaccag ttaggcttcg ccgctgatga gcgcgacttc actctttgcg
    36361 ctgatatgtt caaactcctt ggcgtcaatg aagtccgctt gttaaccaat aacccgaaaa
    36421 aagtcgaaat tctgaccgaa gcggggatta atattgttga acgcgtacca ttgattgtag
    36481 gtcgtaaccc caataacgaa cattatctcg ataccaaagc cgagaaaatg ggccatttgc
    36541 tgaacaaata accctcttgc attgtgtaat taatttgctt gccggaagca aaataaccgg
    36601 caagcaaata cttgttactt caacatatta cgaatgacat aatgcaaaat gccgtcgttc
    36661 tggtagtagg tcaactccgt cgcggtgtcg atacgacaac ggcagggtac gacttcctgg
    36721 ctaccatccg cgcgcgtaag cgtcaccgga accgtcgcgc cgggttgtag attttgcaga
    36781 tcgccaatat caatcttctc ttccccggtt agccctaacg ttttacgcgt tacgccttgc
    36841 ggaaattcca gcggcaggat gcccatgcca attaaattcg aacggtgaat tcgttcaaac
    36901 gattcggcaa tcaccacacg aataccaagc agacgcggac cttttgccgc ccagtcacga
    36961 ctggagcctg atccatactc tttcccggca atcaccgcca gcggcgtttg ctcctgctta
    37021 tagcgcatcg cagcatcata aatagagact acgtcgctgt caggtaaatg ccgcgtcatc
    37081 cccccttcaa cgccaggcac catttcatta cggatgcgaa tattggcgaa ggtgccgcgc
    37141 atcatcactt catggttacc acgccgcgaa ccgtaggagt taaagtcttt tcgctcaaca
    37201 ccccgacctt gtaggtatcg acccgctggg ctgtcgggct taatactgcc cgccggagag
    37261 atatggtcag tggtgactga atcccccagc attgcgagga tccgcgcacc gtgaatatct
    37321 tccacaggtg ctggtgttgc ctgcatttca tcaaagaaag gcgataaacg aatataggtt
    37381 gaatcctcct gccatccgta ggtatcggat cgtgcgacgt taatttcttt ccactctgca
    37441 gtgccttcaa agacttctgc gtactctttg cggaacattt ctgtggagac ttgttctacc
    37501 gcacgggcaa tttcttgtgc cgatggccag atatctttca gatatattgg atcgccttta
    37561 cgatcatgac caataggctc agaagccagg ttgatattca tatttcccgc cagcgcataa
    37621 gcaaccacca gcggcggcga ggccaaccag ttggttttaa ccagcggatg gatacggcct
    37681 tcaaagttac ggttgccgga cagcactgca ccgacggtta aatcgccttt tttgattgcc
    37741 gtttcgatag gatcgggcag cggcccagag ttaccaatac aggtggtaca accgtatccc
    37801 acaaggttaa accccagttc gtcgagatac ggtgtcaatt tcgcttttgc cagatagtca
    37861 gaaacaactt tcgagcctgg tgccagcgac gctttgaccc acggttgccg cttgaggccc
    37921 agagttacgg cttttttcgc cagcaagcct gcagccatca gcacacttgg gttagaggtg
    37981 ttggtgcacg aggttatcgc agcaatgacc acagcgccat caggtaactg atactgatgt
    38041 ccgttcataa cataatcgac cggctggcga tctttatgcg tggcattcac ttccagttcg
    38101 ttactggcgg caaatgcttt tggtacatcg ggcagtgcaa cgcgatcctg agggcgttta
    38161 ggccctgcca ggctcgcttc aacatcattc atatccagtt ctaacgtact ggtaaaaatc
    38221 ggttcatcgc ccgggttacg ccacatgccc tgcgctttgg catatttttc gaccaactcg
    38281 acctgatctt cgctgcgccc gcttaaacgc atgtaatcga gggttacagc atcgattggg
    38341 aagaagccac aggtggcacc atattctggc gacatattgg caatggtggc gcgatccgcc
    38401 aacggtagtg aatccagacc atcaccataa aattcgacga atttccccac cacgccatgt
    38461 ttgcgcagca tttgggtaac agtgagaacc aggtctgtgg cggtaatacc ttcacgtaat
    38521 tttccggtaa gtttaaagcc cactacgtcc gggataagca tggaaaccgg ctggcctaac
    38581 attgcggctt ctgcttcgat gccacccacg ccccacccca gcacgccaag gccgttgatc
    38641 atggtggtgt gcgagtcagt accaacgagt gtatccggat aagcaatcca ttcaccgtcc
    38701 tgcaattcac tccacactgc tttgccgaga tattcgaggt taacctgatg gcaaatgcct
    38761 gtgcctggcg gcacgacgct aaaccgactg aacgcctgct ttccccattt caggaacaca
    38821 taacgttcgt ggttgcgctc catttccagg cgaacgtttt cttcaaatgc ctcatcatca
    38881 ccaaaacgat cgacagtcac cgagtggtca atgaccaggt cgaccggtga gagcggatta
    38941 acctttgcag tatcgccgcc gaggcgttta accgcttcgc gcattgccgc cagatcaaca
    39001 acggcaggta cgccggtaaa gtcctgcatc agcacccttg ccgggcggta ggcaatttca
    39061 cggtcagcat gggcattttt cagccatccc gccagcgcgt ggatatcctc ttcggtaacc
    39121 gaattaccat cctgccagcg cagcaggttt tcgagcaaaa ctttgagtga cttgggtaga
    39181 cgggtgatat cgcccagtga tttggcagca agcggcaggc tgtagtagtg gtaagtttta
    39241 tctttggcct gcaacgtgtc cttactggct tctcgtaggg ttgacgacat agctcctcct
    39301 taatgacagg gttgcgaagc cctgattctc ttcagggtga atattaaaga taacacaaac
    39361 atcgcgtaac gatttgataa caacccaaat tgataaaaga ggagaaagct tgtggcaaag
    39421 caaatgaaaa ccccgccgca gcagggtcag ggatcagtga gtaatgatcc agaggagttc
    39481 ggcccagaag agcatcgaca gagaaaacgc gcccatccag gaccaatatt tcatatttgt
    39541 gttgatcata ggctacaccc ttaacggtta ttactttgtg ttgagataac tcaacctgtt
    39601 attgctgcgg tgtattaaaa gccctgtaat tcattaaaaa tgggtaataa gaatgatgat
    39661 aattattgtt tcggctgatc atcttcagca aacagttcta taaaggcttg ctgctgcgat
    39721 gttagttgcc agttgtctgg aataacgacg gtacattccc gtttcgtttc tgtttcattg
    39781 tcactatgct ggcataatgg tgacgactcc agttgctcct gaccaaccat agttatcccc
    39841 acactttcca aatcagcagt gcgacaacca cccagaaaag tgcagaacca agaaaaaccg
    39901 ccagccaggc tttacgtttt aattcaggat cgcggcgggg ttcctgattt ccagaaggca
    39961 ttgctaacct catataatcg ccatcactta tcagcaagac gttgaacgat tggtgtaatt
    40021 aatcatacga tgagacaaat cctaaagaaa ctttagtcaa aaatccagcg aatttacgaa
    40081 tcgtcttcag tgaataattc aatcttttga cctgaaatag atatttgaaa actggagttt
    40141 gcgaggcggg taattagaca ctactttaca ggtaatttac cctataaata tgagggataa
    40201 aaatattgtg cacggtatac aaataagtaa caaaagagaa tatattacgg caccttcgct
    40261 acataaaagg tgccgataat aacgcaagaa attatttttc cggcagtttt atatctttaa
    40321 acatgacctc aatttcttca ttggaacgca atgcgacagc cgcatcaacg acatcacgcg
    40381 ttaaatgtgg tgcaaaacgc tgaatgaaat catacatata actgcgcaaa aaagtactac
    40441 ggcgaaaacc aattttggtt gtactgtggc tgaagatatc gtgagcatca acgcgcacaa
    40501 ggtcgggatc ggcgaccgga tccaccgcca tgctggcaat gacccctacc cccagcccta
    40561 accggacgta agttttaatg acgtcagcat ccgttgccgt gaaaacgata cgcggcgtta
    40621 accctgcgcg attaaaggca gtatccagtt ctgaacgtcc ggtaaagccg aaggtatatg
    40681 tcaccaacgg atattgcgcc agttcttcaa tggtaatggc ttttttgcct gccagcgggt
    40741 gatccggagt gactacaata gcccgattcc agtggtagca cggtaacatc actaaatctt
    40801 catacagatg cagcgcttct gtggcgatag cgaaatcagc attgccttta gagacggcat
    40861 cagcaatttg tgtcggcgag ccttggtgca tatgcaaaga aacgcgagga taacgctcaa
    40921 taaagccttt gatgacgttt ggtaacgcgt agcgtgcctg ggtatgcgtg gtggcgatat
    40981 acagcgagcc tttatccggc caggtgtgct caccggcaac cgattttatg gcatcaactt
    41041 tcgacaggac ttcacgagcg atacgaatta tttcttgtcc tgctggcgtt acctgcgtca
    41101 ggtgcttgcc gcttcgggaa aaaatttgaa tgcctagctc gtcttccagc attctgactt
    41161 gtttactgat cccgggttgt gatgtgtaaa gtccttccgc tgttgatgag acattcaggt
    41221 tatgattgac cacctcaaca atatagcgaa gttgttgtaa tttcatgtta aaccatccac
    41281 ttagacgcca tcagaacatc gttttttcgt cacacctggc gggtgactaa tacgatttaa
    41341 taataaaaaa ggtgctaact ataaccacta tatcatttat agccttagtg tataggtgtg
    41401 aagaaaaaat aatatgcaat aaaaaagggc cgctttcgcg accctttgtt tataaaaacc
    41461 tgacagaatt aaaggttatt ttttcccttc aacccatttg ccatcaacat aaaatgctga
    41521 ccagccagtc gcctttccgt ctttttccga agagacatat tgctgtttgg ttttacggct
    41581 aaagcgaacc atagtcttat taccttccgg atcctgctgc ggcgcatcgg ccagataacg
    41641 cagtttttcc ggcagacgat cgcggaagcg atacagctct tccaccagcg gcgcacgcgt
    41701 ttcacgcgat ttcgggaaag tgttggcagc caggaaaaca cccgcagcgc cgtcacgcag
    41761 cacgaaataa gcatctgatt tttcgcacgg cagctcaggt aatggcaccg gatcttcttt
    41821 cggtggtgcc acttcgccgt tacgtaaaat cttacgcgtg tttttacact cttcgtttgt
    41881 gcaggccata tatttaccga aacgccccat tttcaggtgc atttcagaac cacatttttc
    41941 acactcaacg atcgggccgt cataaccttt aatgcggaat tcgccctctt cgatctcgta
    42001 accgtcgcag gttgggttat taccacagac atgcaactta cgtttcggat cgatgagata
    42061 gctgtccatc gccgtgccgc atttcgggca acgacgtttt gcgcgcagcg cgttggtttc
    42121 agcgtcttcg ccttccagca cgttcagcac ttcgttttcc ggcaccaggt taatggttgt
    42181 tttgcaacgc tctttcggcg gcagcgcata gccagaacag ccaaggaata ccccggtgct
    42241 cgctgtgcga atccccattt tgcgaccaca ggtcgggcag tcgatgctgg tcagaaccat
    42301 ctggttcgga cgcatacccc cctcttccgg atctttttca gctttatcta actgctgagt
    42361 gaaatccgag aagaagtgat ccagtacagc tttccactct gcttcgtgat ttgccacctg
    42421 gtcaaggctg ttttccatct gcgcggtgaa gtcgtagttc attaactcgc ggaaattctc
    42481 ttccaggcga tcggtgacga tttcgcccat tttttccgca tagaaacgac gattttctac
    42541 tcgcacatag ccacgatcct gaatggtcga aatgatcgac gcataggtag acggacgacc
    42601 gataccgcgt ttttcaagct ctttaaccag cgatgcttca ctgaaacggg ctggcggttt
    42661 ggtaaagtgc tgggctggtg tcagttcaac gagcgtcaga gcatcgcctt tatcgactgc
    42721 cggtaagata cgatcttcat cgcctttacg cagtgcaggc atcacttttg tccagccatc
    42781 aaagcgcaaa atacgaccgc gtgctttcag gcggaaatcg cccgcaccaa cggtcagcgt
    42841 cgtggagtca tatttcgctg gggtcatctg gcaagcaacg aactgacgcc agattaactg
    42901 gtacagtttc tgcgcatctg cttccatatc cttcaacgat tccgccatca cattgacgtc
    42961 agaaggacga atcgcttcgt gcgcttcctg tgagttttct ttgctggcgt actgattcgg
    43021 actttccggc agatatttct taccaaaatt atcgctgata taaccgcgaa ccatatttac
    43081 cgcgtcctga ctcaggttag tagagtcggt acgcatgtaa gtgatatagc ctgcttcata
    43141 caaacgctgc gccatcatca tggttttttt cacgccaaat ccaagacggg tgctggcagc
    43201 ttgttgcagc gtagaggtaa taaaaggagc gccaggttta ctggttgtcg gtttgtcttc
    43261 acgttccagc acgctgtagc gcgctttttc cagcagactt accgcagcct gagtttgttc
    43321 tttgttgacc ggacggaacg gtttgtcgtt ctgatgagtc acctgcaacg ccaacgcatc
    43381 accagatggc gtggtcgtgc tggcatcgac ttcccagaac tcttccggca cgaacgcttt
    43441 aatttcacgc tcacgctcga ccaccaggcg gactgccacc gactgcacac gaccggcaga
    43501 cagaccacga gcgatctttt tccatagcag cggcgaaacc atatacccca ccacgcggtc
    43561 cataaagcga cgcgcctgct gggcattaac acgatcaata ttcagctcac ccggtttgtt
    43621 aaacgcctgg cggatcgcgt ttttagtaat ttcgttaaac accactcggc tatagcgcgc
    43681 atcatcaccc ccaataactt cccgcaggtg ccatgcaatg gcttcccctt cgcggtcaag
    43741 gtcggttgcg agatagatgt ggtcggcttt ttcagccagt tgtttcagtt cagagacgac
    43801 cttctcttta ccaggcaaca cttcatagtg cgcctcccaa ttgtgccacg ggtcaacccc
    43861 catacggttg acgagagcgc cacgttcatc ctttttaggc tttttagccg tcttggtgga
    43921 ggtagagtcg gcactctttt tggcagctga gccactggtc ggcaaatcgc ggatgtgacc
    43981 gacgctggat ttcaccacgt agtcactacc cagatacttg ttgatcgttt tggcttttgc
    44041 cggggactca acgatgacaa gagctttacc catattcacc ttacctaatt tgattcttcc
    44101 aggaatgcgt cgcacgttga taaaccttcc actggcaacg agttaccgat atggactcgt
    44161 tctctgcgga tatcaacccc ttgtcgtgac tctgttcacg aaaattcagg tgactgagtt
    44221 aacaggtctt ggcctacagg cgctatagca cgacggaatt ttggtcgaat gtcaagcaaa
    44281 tctattgcca gattgacgaa agcgtcacac tgtacctgat aaaatccgat acgcaacttt
    44341 attagcatgc aaaatccaaa gttgccagta aggcgctctt gccaccagcg tggtatattt
    44401 gtctgcaagc catgcctggc gtagactaat gtccttgttt aaggagtaaa taatgaacaa
    44461 agagacacaa cccatcgacc gcgaaacgct gttgaaagag gccaacaaaa tcattcgtga
    44521 gcatgaggat acgctcgcag ggattgaagc caccggcgtc acgcagcgta atggcgtgct
    44581 ggtatttacc ggcgactact ttttagatga acaaggattg ccaacagcca aaagcaccgc
    44641 ggtgtttaat atgttcaaac acctggccca tgtgctttca gaaaaatatc acctggttga
    44701 ttaaagcaaa acgcgaggtc ttaccctcgc gtttgtcttt tacatcaatg gcttttgccc
    44761 ccgctgccac cagcgtaaca acaatcgatc ggcgctctct gccgcgctgc cggtgaatcg
    44821 gtcaatgagt cgtttacgct gcatatagcg tacattgacc acttcacggc cttccatcag
    44881 gctaagaata acttcatcac tggtgttgat ttcatcaacc aggcctttct ctactgcctg
    44941 ttgtccgtac cagtgttccc ccgttgccac ctgttcaata tccagagacg gacgcatacg
    45001 cttcacaaaa tctttaaata actgatgcgt ttcgttcagc tcttcgcgga atttctcccg
    45061 cccttcttcg gtattttcac ccagcaaggt cagcgtacgc ttatactgcc cggcggtgtg
    45121 cagttcgata tcaatatctt tgcttttcag gaagcggtta aagttgggca tttgcgccac
    45181 caccccaatg gaacccacaa tagcaaacgg tgcggaaaca attttgtccg ccacacaggc
    45241 catcatgtaa ccgccgctgg cagcaacttt gtctaccgta acagttaaag gaatgttttt
    45301 atcacgcaga cgctgcagct gcgaagccgc caacccgtaa ccatgcacca tgccaccagg
    45361 gctttccaga cggagcacaa cctgatcctg cggtttgaat gctgcgagta cagccgttat
    45421 ctcttcacgt agcgagttca cttcatgggc gtccatgctg cctttaaaat ccagcaccca
    45481 gacgcggggt ttactgtcag ttgccacctc gcccagtttg gctttcgctt ttgctgcttt
    45541 cgcttcttgc ttatgctttt tcttctgcgc tttgtgccac tgtttttgct gatgtgagtc
    45601 catcagcgcc gcggccagtt cttctttcat ctccttatac tgttcgctga gattgttgac
    45661 ccgtaactcg ccacgctggc gtttattacg ttgagcaaca ttgacaataa tggcggcaat
    45721 cgccgcaatc gctagcacaa cggtaacgat tttcgccaaa aacaaaccat attcagacaa
    45781 caattccacg agtccccacc ttggttaaac aacgctttgg ctcacagttt acaacaggca
    45841 tgttaagccg tctcgcagag atgttattgc agcgaaagta tgacaaagta tccattttgc
    45901 ggtgttatcg ccccaggctg attgaaatgc ccttctgttt caggcataaa gccccaaagt
    45961 cataaagtac actggcagcg cgacagacaa cgtcgttgag gagtagccat gcattaccag
    46021 ccaaaacaag atttactcaa tgatcgcatt atcctggtga cgggagccag tgatggtatt
    46081 ggtcgtgaag ccgcgatgac gtatgcacgc tatggtgcga cagtgattct gttgggccgt
    46141 aatgaagaaa aattacgtca ggtagccagc cacataaacg aagaaactgg gcatcagcca
    46201 cagtggttta ttctcgattt gctgacctgc aggtccgaag attgccaaca actggcacag
    46261 cgcattgccg ttaattatcc gcgtctggat ggtgttttgc ataatgccgg attgctcggc
    46321 gatgtttgtc caatgagcga acaaaatcca caggtctggc aggacgtcat acaggtcaac
    46381 gttaatgcca cctttatgct cacccaggca ctgcttcctt tattactcaa atcggacgcc
    46441 ggttcactgg tctttacttc atcaagcgtt ggacgtcagg gacgagccaa ctggggcgca
    46501 tatgcagcgt cgaaatttgc caccgaaggg atgatgcagg tactggccga tgaatatcag
    46561 cagcgcctgc gcgtcaactg cattaaccca ggcggtacgc gcaccgcaat gcgtgccagc
    46621 gccttcccga ccgaagatcc acagaaactt aaaacacccg ctgatatcat gccgctctac
    46681 ctctggctga tgggcgatga cagccgccgt aaaaccggca tgacctttga cgcccaaccg
    46741 ggccgtaaac caggaatttc ccaatgagtg atgaacgcta ccaacagcgt cagcagcgag
    46801 tgaaagaaaa agtagatgct cgtgtggccc aggcccagga tgaacgcggt attatcatcg
    46861 tctttaccgg caatggaaaa ggcaaaacca ccgcggcatt tggtacggca acacgcgcag
    46921 ttggtcatgg aaaaaaagtg ggcgtcgtgc agtttattaa aggcacctgg cctaatggcg
    46981 aacgcaatct gctggagcca catggcgttg agtttcaggt gatggcaacg ggctttacct
    47041 gggatacaca aaaccgcgag tctgataccg ccgcctgccg cgaagtctgg caacatgcaa
    47101 agcggatgct tgctgattcc tcactggata tggttttgct tgatgagctg acttatatgg
    47161 tggcgtatga ctatttgcca ctggaagaag tggtgcaggc gttaaatgaa cgtccacatc
    47221 aacagacggt gattatcacg ggtcgaggct gtcatcggga tattcttgaa ctggcggata
    47281 cggtaagtga gctgcgcccc atcaaacatg cgtttgatgc cggtgttaag gcgcaaatag
    47341 ggattgatta ttaatctgta aaaatgccgg atatgacgtc cggcatactt tgattaaccg
    47401 ttattattac gtccgccaga acgacgaccg ccgctcacct gactgtgacg tttcaccgca
    47461 cgacgaatct gattcgcctt catgcgacga cggtcttttt ctacagcgac tttagagctg
    47521 gtttctggcg gtagctccac cagttcgcgc agatagttag tctgggcgag gtccagctcc
    47581 gtccagccac cgcgtggcag accttttggc agtgggatat caccgtaacg aacacggatc
    47641 aggcggctca cctgcacacc aaccgcttcc cacagacgac gaacttcacg gttacggcct
    47701 tcggtcagag tcacgttgta ccactggttg atcccttcgc cgccgctgaa cttgatggtt
    47761 ttaaaagctg ccggaccatc ttccaactgc acgccacgac tcaaatcacg cagtttcgcg
    47821 tcgtcaacct gaccaaatac acgcacggca tattcacgtt caacttcacg gcttgggtgc
    47881 attaaacggt ttgccagttc accatcggtg gtgaacagca gcagaccaca ggtattaacg
    47941 tccaagcgac ccacggcaat ccagcgtgcg ccacgcagtt ttggcagacg gtcaaacacc
    48001 gttggacgtc cttccggatc gttacgggta cacaactcac cttccggttt gtaataggcc
    48061 agaacgcgac aaatttgttc agcggactca cgtaccgaaa tcaggtgacc atcgatacgg
    48121 attttcaatc caggggtaac ttcaacgcga tcgccgagtt tagcaatttt gccatcaaca
    48181 ctcacacgac cggcttcaat aatagattcg atttcacggc gagaaccgtg gccggcacgc
    48241 gccagcactt tctgtagctt ttcgctcata gagcttcctt ttggtgtcgc cttcccaggc
    48301 gtctgatatg cttttgtgca acagtgagtc agaacttaca tctgactcac tgatagtaaa
    48361 gtctgtttga actcttcagg ccttaagtaa acgacccgac gttttaactg gcctggcttt
    48421 gaggcgcaac atactacact atctttatgg ttaaagcttg tttcgattaa catcagcttg
    48481 attaccagcc acca
//
LOCUS       DAZUCE010000011        44208 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZUCE010000011 DAZUCE010000000
VERSION     DAZUCE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.011"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            49..1338
                     /gene="uraA"
                     /locus_tag="ACHY03_000574"
     CDS             49..1338
                     /gene="uraA"
                     /locus_tag="ACHY03_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFS3903851.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     IVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            1433..2134
                     /gene="hda"
                     /locus_tag="ACHY03_000575"
     CDS             1433..2134
                     /gene="hda"
                     /locus_tag="ACHY03_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFS3903852.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(2272..2631)
                     /gene="arsC"
                     /locus_tag="ACHY03_000576"
     CDS             complement(2272..2631)
                     /gene="arsC"
                     /locus_tag="ACHY03_000576"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFS3903853.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2652..4115)
                     /gene="bepA"
                     /locus_tag="ACHY03_000577"
     CDS             complement(2652..4115)
                     /gene="bepA"
                     /locus_tag="ACHY03_000577"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFS3903854.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4328..5389
                     /gene="yfgO"
                     /locus_tag="ACHY03_000578"
     CDS             4328..5389
                     /gene="yfgO"
                     /locus_tag="ACHY03_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFS3903855.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(5427..6275)
                     /gene="focB"
                     /locus_tag="ACHY03_000579"
     CDS             complement(5427..6275)
                     /gene="focB"
                     /locus_tag="ACHY03_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFS3903856.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTPFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(6297..8309)
                     /gene="hyfR"
                     /locus_tag="ACHY03_000580"
     CDS             complement(6297..8309)
                     /gene="hyfR"
                     /locus_tag="ACHY03_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFS3903857.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(8339..8752)
                     /gene="hyfJ"
                     /locus_tag="ACHY03_000581"
     CDS             complement(8339..8752)
                     /gene="hyfJ"
                     /locus_tag="ACHY03_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFS3903858.1"
                     /translation="MTEECGEIVFWTLRKKFIASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPQTEYEDWLAQVEEKQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSAWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(8745..9503)
                     /gene="hyfI"
                     /locus_tag="ACHY03_000582"
     CDS             complement(8745..9503)
                     /gene="hyfI"
                     /locus_tag="ACHY03_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFS3903859.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIHRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(9500..10045)
                     /gene="hyfH"
                     /locus_tag="ACHY03_000583"
     CDS             complement(9500..10045)
                     /gene="hyfH"
                     /locus_tag="ACHY03_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFS3903860.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(10055..11770)
                     /gene="hyfG"
                     /locus_tag="ACHY03_000584"
     CDS             complement(10055..11770)
                     /gene="hyfG"
                     /locus_tag="ACHY03_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFS3903861.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGDSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALRIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(11760..13340)
                     /gene="hyfF"
                     /locus_tag="ACHY03_000585"
     CDS             complement(11760..13340)
                     /gene="hyfF"
                     /locus_tag="ACHY03_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFS3903862.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHCLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(13345..13995)
                     /gene="hyfE"
                     /locus_tag="ACHY03_000586"
     CDS             complement(13345..13995)
                     /gene="hyfE"
                     /locus_tag="ACHY03_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFS3903863.1"
                     /translation="MTGSTIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(14007..15446)
                     /gene="hyfD"
                     /locus_tag="ACHY03_000587"
     CDS             complement(14007..15446)
                     /gene="hyfD"
                     /locus_tag="ACHY03_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFS3903864.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     SQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(15463..16410)
                     /gene="hyfC"
                     /locus_tag="ACHY03_000588"
     CDS             complement(15463..16410)
                     /gene="hyfC"
                     /locus_tag="ACHY03_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFS3903865.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLSMALPLFIAVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQEVQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16421..18439)
                     /gene="hyfB"
                     /locus_tag="ACHY03_000589"
     CDS             complement(16421..18439)
                     /gene="hyfB"
                     /locus_tag="ACHY03_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFS3903866.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVSIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYSRENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(18439..19056)
                     /gene="hyfA"
                     /locus_tag="ACHY03_000590"
     CDS             complement(18439..19056)
                     /gene="hyfA"
                     /locus_tag="ACHY03_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFS3903867.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCDFLPQGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(19309..19779)
                     /gene="bcp"
                     /locus_tag="ACHY03_000591"
     CDS             complement(19309..19779)
                     /gene="bcp"
                     /locus_tag="ACHY03_000591"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFS3903868.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHHVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(19779..20351)
                     /gene="gcvR"
                     /locus_tag="ACHY03_000592"
     CDS             complement(19779..20351)
                     /gene="gcvR"
                     /locus_tag="ACHY03_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFS3903869.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            20497..21375
                     /gene="dapA"
                     /locus_tag="ACHY03_000593"
     CDS             20497..21375
                     /gene="dapA"
                     /locus_tag="ACHY03_000593"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFS3903870.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21392..22426
                     /gene="bamC"
                     /locus_tag="ACHY03_000594"
     CDS             21392..22426
                     /gene="bamC"
                     /locus_tag="ACHY03_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFS3903871.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            22639..23352
                     /gene="purC"
                     /locus_tag="ACHY03_000595"
     CDS             22639..23352
                     /gene="purC"
                     /locus_tag="ACHY03_000595"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFS3903872.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            23520..24383
                     /gene="ypfJ"
                     /locus_tag="ACHY03_000596"
     CDS             23520..24383
                     /gene="ypfJ"
                     /locus_tag="ACHY03_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFS3903873.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            24398..26413
                     /gene="tmcA"
                     /locus_tag="ACHY03_000597"
     CDS             24398..26413
                     /gene="tmcA"
                     /locus_tag="ACHY03_000597"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFS3903874.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQILQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSNAL
                     VFDDENFTHTPQGNIVISAFEQTLWRIEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            26487..27185
                     /gene="ypfH"
                     /locus_tag="ACHY03_000598"
     CDS             26487..27185
                     /gene="ypfH"
                     /locus_tag="ACHY03_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS3903875.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(27266..27466)
                     /gene="ypfN"
                     /locus_tag="ACHY03_000599"
     CDS             complement(27266..27466)
                     /gene="ypfN"
                     /locus_tag="ACHY03_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFS3903876.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(27494..28621)
                     /gene="dapE"
                     /locus_tag="ACHY03_000600"
     CDS             complement(27494..28621)
                     /gene="dapE"
                     /locus_tag="ACHY03_000600"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFS3903877.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVDIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(28625..28981)
                     /gene="yffB"
                     /locus_tag="ACHY03_000601"
     CDS             complement(28625..28981)
                     /gene="yffB"
                     /locus_tag="ACHY03_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFS3903878.1"
                     /translation="MVTLYGIKNCDTIKKARRWLETNNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            29362..29421
                     /gene="ypfM"
                     /locus_tag="ACHY03_000602"
     CDS             29362..29421
                     /gene="ypfM"
                     /locus_tag="ACHY03_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFS3903879.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(29520..32633)
                     /gene="acrD"
                     /locus_tag="ACHY03_000603"
     CDS             complement(29520..32633)
                     /gene="acrD"
                     /locus_tag="ACHY03_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFS3903880.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPKLTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(32797..34497)
                     /gene="narQ"
                     /locus_tag="ACHY03_000604"
     CDS             complement(32797..34497)
                     /gene="narQ"
                     /locus_tag="ACHY03_000604"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFS3903881.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            34703..36682
                     /gene="aegA"
                     /locus_tag="ACHY03_000605"
     CDS             34703..36682
                     /gene="aegA"
                     /locus_tag="ACHY03_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFS3903882.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRGAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            36750..37325
                     /gene="nudK"
                     /locus_tag="ACHY03_000606"
     CDS             36750..37325
                     /gene="nudK"
                     /locus_tag="ACHY03_000606"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFS3903883.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            37451..38494
                     /gene="ypfG"
                     /locus_tag="ACHY03_000607"
     CDS             37451..38494
                     /gene="ypfG"
                     /locus_tag="ACHY03_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFS3903884.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITEFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38589..40592)
                     /gene="tkt"
                     /locus_tag="ACHY03_000608"
     CDS             complement(38589..40592)
                     /gene="tkt"
                     /locus_tag="ACHY03_000608"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS3903885.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(40612..41562)
                     /gene="tal"
                     /locus_tag="ACHY03_000609"
     CDS             complement(40612..41562)
                     /gene="tal"
                     /locus_tag="ACHY03_000609"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFS3903886.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            41851..44130
                     /gene="maeB"
                     /locus_tag="ACHY03_000610"
     CDS             41851..44130
                     /gene="maeB"
                     /locus_tag="ACHY03_000610"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFS3903887.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT        10648 a        11561 c        11825 g        10174 t
ORIGIN      
        1 ttttttttga gtaaagcgcc tataacacat aatacagagg ataatactat gacgcgccgt
       61 gctatcgggg tgagtgaaag accgccactt ttacagacaa tcccgcttag tttgcaacat
      121 ttgttcgcca tgtttggtgc aaccgtcctg gtgcccgtct tatttcatat taacccggcg
      181 actgtactgt tatttaacgg tattggaacg ctgctgtatc tcttcatctg taaagggaaa
      241 attccggctt atcttggttc cagctttgcc tttatttcac cggtattgct actgttgccg
      301 ttggggtatg aagtcgcgct gggcggcttt attatgtgcg gcgtgctgtt ctgcattgtt
      361 tcttttatcg tgaagaaagc ggggaccggc tggctggacg tgctgtttcc acctgcggca
      421 atgggcgcaa tcgttgccgt catcggtctg gagctggcgg gcgtagctgc cggtatggcg
      481 ggtttgctcc cggctgaagg gcaaacgcca gactccaaaa ccatcatcat ctctattacc
      541 accctggcgg tcacggtttt aggttccgtg ctgtttcgtg gtttcctggc aattatcccg
      601 attttaattg gcgtgctggt ggggtacgcg ctctctttcg caatgggaat tgtcgatacc
      661 acgccgatta ttaatgctca ctggtttgcg ctgccaaccc tctatacgcc gcgcttcgag
      721 tggtttgcca ttctgactat tctgccagcg gcgttagtgg ttattgccga acacgtaggg
      781 cacctggtag taacggctaa tatcgtcaaa aaagatctgc tgcgcgatcc aggtctgcac
      841 cgttcgatgt ttgctaacgg cctgtcgacc gtgatctccg gcttctttgg ctctacgcca
      901 aataccactt acggagaaaa cattggcgtg atggcgatca cccgtgttta cagtacctgg
      961 gttatcggcg gggcggcgat tttcgctatc ctgctttcct gcgtcggtaa actggctgcc
     1021 gctatccaga tgatcccatt gccggtaatg ggcggcgttt cgctgctgct ttatggtgtc
     1081 atcggtgctt ccggtattcg tgttttgatc gaatcgaaag tggattacaa caaagcacag
     1141 aacctgatcc tgacttccgt gattttgatc atcggcgtca gtggcgcgaa ggtaaacatc
     1201 ggcgcggcgg agttgaaagg tatggcgctg gcgaccatcg tcggtatcgg cttaagtttg
     1261 attttcaaac tgattagtgt gctgcgccct gaagaagtgg tgctggacgc agaagacgcg
     1321 gatataacag acaaataatg taccgaccgg gcagtgttgc tgcccggttc aaacatcatg
     1381 ggattctgtg gtaaacttct cgcgattttg tgaaatcctg gttgaggtat ctctgaacac
     1441 accggcacag ctctctttgc cactttatct tcctgacgac gaaacctttg caagtttctg
     1501 gccgggggat aactcctctt tactggccgc gctgcaaaac gtgctgcgtc aggaacatag
     1561 cggttacatc tatctctggg cacgcgaagg cgcggggcgc agccatctgc tgcacgcggc
     1621 ttgcgcggaa ttgtcgcagc gtggcgatgc ggtgggctat gtcccgctgg ataaacgcac
     1681 ctggtttgtt ccggaagtgc tcgacggtat ggagcatttg tcgctggtct gtatcgacaa
     1741 cattgagtgt attgcaggcg atgagttgtg ggagatggcg attttcgatc tctacaatcg
     1801 aattctggaa tcgggcaaaa cacggttgtt gatcaccggc gatcgtccac cgcggcagtt
     1861 gaatctggga ttaccggatc tcgcgtcgcg actcgactgg gggcagatct acaaattgca
     1921 gccactttct gatgaagata agttgcaggc gctacagtta cgcgcgcgtt tgcgtggttt
     1981 tgaactgccg gaagatgtgg ggcgtttctt gctgaagcgg ctcgacagag aaatgcgcac
     2041 gctatttatg acgttggatc agttggatcg tgcgtcgatt accgcgcaac gtaagctgac
     2101 cattccgttt gtgaaagaaa ttctgaagtt gtagataagg tgtttattgt cggatgcggc
     2161 gcgaacgcct tatccgaact acgcgaagtt aatccatagt attcgtaggc cggataagac
     2221 gtggcaagcg tcgcatccgg caatgtgctt caactagcga tggagcacat cttaacccac
     2281 gatttccagt acctgctccg gtggacgacc aatccgtgct ttgccattcg cgaccacaat
     2341 cgggcgctcc atcagcttcg gattatccac cattgcctga atcagcgcct cttcgctaag
     2401 cgaactgtca gccaggttca gttctttata aagatcctct ttctggcgca tcaattcacg
     2461 ggcgctattc atccccagca ttttcagcaa atcgcgcagc gttgccgcat cggcgggtgt
     2521 ctcaaggtag agcaccactt ctggctccac gccgttttct ttcagcaggt tcagcgtttc
     2581 ccggctcttt gaacagcgtg ggttatggta gatttttacc tgtttggtca ttgttcttcc
     2641 tttaatgcga attacatctt ggtataaggc ttaaagcgtt cctgcagctg gcgcaactgg
     2701 tcgatgcgcg catcgtaacg cgcttgttgc aggctgccta atttcacctg cgaactggcg
     2761 ctactcaaca gcgaaatggc ctgatcgagt cgtccggcga gcgcataacc ttctgctcgc
     2821 gcagccagtt cctgatcgcg gttatttagc gcggcttccg cctgtgccag taaatcccag
     2881 ccgttgctgt catctttatt attaaaggtg tagcgattga gaatattggc cgcttcttgt
     2941 ggttgaccgc cttgcagata agcgtttgcc aggttgagct gcaacaccgg attggtgcgc
     3001 aaatcgcggg catttttcag acgattgatc gcctcattgg ctttgttttg cccaagatcg
     3061 atatcagtag ccagatcgag ataccatgcg ttgccaggtt ctgccgccag taacggttgc
     3121 agcgttttac gcgcctcgtc gtatttattg gcttccatcg cctgtaaagc acgaccatat
     3181 tgcgccgctc gttgctgacg aacgtttcct ttcgcccatt catccagcaa atcactggtg
     3241 agctggttac gtccggaatt atacatcccc agtgtgcgcg ctttcgccag atagaaatct
     3301 tccgacgact gcaccaccat cgggcgcatc tgattagcac ggttgcgggc atctgccaga
     3361 cgactttccg gcaacgggtg agtcagtaaa atttccggcg ggcgcgagga gtaacgcgcc
     3421 tgatcgagta atttttcgag gaaggttggc atcgcctgcg gatcgaatcc cgagcgttgc
     3481 agcacctgaa taccaatgcg gtccgcttcc tgttcatttt gctgggtgaa actgatcatc
     3541 ccctgacgcg ttcccgccag tgtaccggtc agcgccgcca tccccgcctg cggactggcc
     3601 atcgccagta aaatagaacc taacgcgccg acccaggtca gcggcgcgct gcgctgctga
     3661 tcttccatcg ctcgcgccag gtgacgttgg gtgacgtggg agatttcgtg cgccataact
     3721 gaagccagtt gactttcgtt atcggaataa cggaacaggg cagagtgcag caccacgttg
     3781 ccgccaaaga aagcaaaggc gttaatttcg tcgttgttga tcagaaaaaa atgaaacggt
     3841 gtcttaaccg aattggcatg cgaaaccaga cgcatcccca gcgaattaat atattgcgtt
     3901 aacagcgggt cattaattaa cggcgcgctg ccgcgtagct ggcggacata atagtcgccc
     3961 atctgcattt cctgaccaat ggaaagcgtg cttcctgcgg aggttcccat atccggcaag
     4021 gtgtctgcac tgtcggcaaa tgccggggct acctgaccaa tggtcatagc agcaatgagg
     4081 gttgcaacca ggtttttttt caactgcctg aacataacct ctatcctgta tttctgaaga
     4141 tagtcatttg accgatactg tgaaatatcg ttcccaccgc gccgcctttg agtgtaacgg
     4201 cgaagattag cccactcaaa tgacttttca gtttcctgaa tccgtcataa aaagcgaagt
     4261 cttttttgtg acatttcgat acaattgcgg atcgcaatcc cgctcttgag gttcagggaa
     4321 ggtctttatg ctcgaaatgt tgatgcaatg gtatcgccgc cgttttagcg acccggaagc
     4381 gattgccttg ctggttattt tagttgccgg atttggcatt atctttttct ttagtggcct
     4441 gcttgctccg ttgctggtgg ctattgtgct ggcctatttg ctggaatggc caactgtgcg
     4501 cctgcaatct attggctgct cccgccgctg ggcgacgtcg attgtattgg tggttttcgt
     4561 cggtatattg ctactgatgg cgttcgtggt actgcctatc gcctggcagc agggcatcta
     4621 cttaatccgc gatatgccgg ggatgctcaa taagctttct gactttgccg ccacgttgcc
     4681 gcgccgctat ccggcgttaa tggatgcggg cattattgat gcaatggccg aaaatatgcg
     4741 cagtcggatg ctgaccatgg gcgattcggt ggtgaaaatt tccctcgcct cgctggtcgg
     4801 tttgctgacc attgccgtct atctggtgct ggtaccattg atggtcttct tcctgctgaa
     4861 agacaaagag cagatgctga atgccgttcg ccgggtgctg ccgcgtaacc gtggactggc
     4921 aggacaggtg tggaaggaga tgaatcaaca aatcaccaac tatatccgcg gcaaagtgct
     4981 ggagatgatc gtagtaggga tcgccacctg gctgggattc ttactcttcg ggctgaacta
     5041 ttcgctgctg ctggcggtgc tggtcggctt ctcggtactt attccgtata ttggcgcatt
     5101 tgtggtgacc attccggtgg ttggcgtggc gctattccag tttggtgcag gcactgaatt
     5161 ctggagctgt ttcgcggtgt atctgatcat tcaggcgctt gacggcaacc tgttagtacc
     5221 ggtgttgttc tccgaagcgg ttaatctgca tccgctggtg attattttat cggtggtgat
     5281 cttcggtggt ttgtggggat tctggggcgt attcttcgcc attccattgg cgacgctgat
     5341 caaagccgtg atccacgcct ggcccgatgg gcaaatagcg caagaataat cttcagcccg
     5401 acgaactgtt tcgccgggct gatttttcag gattcctgac gtaaataaat agcccgataa
     5461 cacatactca ccagcactgc accgccgata atattaccca gcatcaccgg gagcagattg
     5521 gcggtaataa aatggctgac cgtcagtgca ggaaaattgt ctgcgctact gtgcgccagt
     5581 tgccagaagg gggttggggc aaaatggcga atggcaatgg cgaacggaat cacaaacaaa
     5641 ttggcgatac agtgctcaaa gccactggcg acaaacaggg tgatgggcaa aatcatggcg
     5701 acgattttgt cgcataatga gcgcccgcaa taactcatcc acagcgccag gcaaaccatc
     5761 agattgcaca taatgccgag gctgacagat tcagtaaatg tatgatgcat tttgccctcg
     5821 gcgcagtgta aaaccgcgac tccccacatc gcgttttcac tcatcaccag cccggaaaac
     5881 cagattaaca aactgaaaca ggcaatacct gccagattac cgcaggccac cagaagtgcg
     5941 ttaatcagcc aagttcgcca actaataacg ccccgacttt ttgccatcac cgtcattacc
     6001 gacgaggtga acaggctggt gccgcaaacc gccagcaaaa taaagccgag tgtaaagcaa
     6061 aggccgccca ccagatgggt taatgcctgt gaagacgggg catcggcgat aacggaaagg
     6121 taaaaagtaa atccgatcgc cataaacacg ccagcggaca tcgccattaa aaagaccgac
     6181 actttactgc gggcaatttt atgggcggca atccgttcag cgattgccgc ttttctggcg
     6241 ctcaactgca agtcgaaaga gagtttgttt ctcatgcata ctcctgcaaa agcagattac
     6301 aacacctcgc gaaccgagat ccccagacgc tgcattcgtg acagcagcgt ggtgcgcttc
     6361 atccctaatc gtgtcgccgc gccacggggc ccggcaacaa tgccattggt ttctcgcaat
     6421 acctgaacaa tgcgctgacg ttcttcttcg tcattctctg gcgtcgtcgg gtgcagcaac
     6481 tgcgccattg aactgcgaag cgctgaatct tcatttagcg tcggcagtaa acggctttgt
     6541 cggacattta gatgtaaatt cagactgtta ccacgagtca acagtaccgc ccgctcaatc
     6601 acgttttcca gctcgcgcac gttgcccggc caatcccacg acatcaactg gcgtagtgcc
     6661 tcggtcggga tggcgtcaat tgcgcgattc atatggcgcg ccattttttg cgtgaaatgt
     6721 tttgctaaaa gagggatatc ttccggacgg tcgcgtagcg gcggcaattc cagtgggaag
     6781 acattcaggc gataaaagag atcgctgcga aactggcgat cttcaaccat ttgccacaaa
     6841 tcacggttgg tggcggcaat gactctgaca ttcaccggga tcgttctact cccgccgaga
     6901 cgctcaatct cccgctcctg caatacgcgc agcagtttag gctgaagttc taacggcaga
     6961 tcgccaattt catcgagaaa caacgtgccg ccatcggcaa tttcaaaacg accacgatgg
     7021 gtattaatcg caccagtaaa tgcccctttg tcatgaccga ataactcact ttccagaaga
     7081 ctggcgggga tggcagcgca gttgattttg accagcggct tgtcgcgtcg cgggctaagt
     7141 tgatggatcg ctctggcgat cacctctttg ccagttccgg tttcaccgca aatcaacacc
     7201 gtactgtcgc tcttcgccac aatatctacc tgctggagca ggtcttccat tgcctggctt
     7261 tgatagataa tgtcaccgac gcccagattc gaaaggagct gctcgctaag ctggtggttt
     7321 tcttgctgca aactttcctg caaatcggtc atgctacgcc aggcatcggc attgccaacg
     7381 gcaatagcga tgcgatcggc tatgtgttgt agtagctggc agttttcctc actaaagaga
     7441 gtggaagagg tatgcgccag caacaatgca cccggtgtat ggttgccaaa ggtaagcggt
     7501 ataaggagcg cagattcgca gccgttagat gcaagtaagt gtaataacgg gtcgcgttgc
     7561 cagagaaaca gatcgtctgc acggtgcgtc aaggtcggtt gtcgggtttg tagcgtttgt
     7621 gtcagcaata cactgtcgcc aggcatattg cgtggcagac acgcacaatg tgaggcagaa
     7681 agatcgctgc accacaggct gaacttctcg ttctttcgat gatcgcccag taccatactg
     7741 actgaagcca gaccgaaaaa atgatggatc tcacgagcga cgtcagcgat cagatcgtcg
     7801 agatcaagat gtgacagcac tgcattagtg atatccacca gtacccgatg atgatcgcga
     7861 tctttctcca gcaaacgcag cgccacctgt tgttcttcat cattgagcca tgattgcaga
     7921 accggggtga taatgcgtgc cagccagtta aacagtgtgc aatcctgggg agtaaaacgg
     7981 ctgccgtggg tacgaataaa gcgtagctcc gccattccac tactgccgag aaggacggcg
     8041 tgacgcgaat aatcgaccat ctcgccctga tactctactg ttgccgtcac atcgctgcca
     8101 tcatcatggt agcggtaata gaagttgctt ttgagaacat gcagttcaat aagttgtacc
     8161 gggctgaacg gcggcggcag cgggatgaag gcgcgtaata aagacgcctt gccgtgtttc
     8221 tgcgctaacc gctccgcgag cattccgttt accgcttcaa ttgttattcc ttgcggcggg
     8281 gcaaacatcg cctcgtctga catagccatc aacagtcctt attaatgaga aatccttctc
     8341 acgcaatttt tctggccatc agatagatgg ccggttcctg cacaatttca tccaacagat
     8401 gaatgaattg aatgctacgc gcctgccacg ccgacgtcgc gtcatctgcc agtggcgcga
     8461 atgcccgggt caacagggcg gtgtggctgg cgtcaataac aatctcacca aaggtcatca
     8521 cccccagcat cttacgtcgg gcttgcttct cttcgacctg tgcaagccaa tcttcgtatt
     8581 ccgtctgtgg gcagcggaag gcgacattca gacaatcaat cacgccaacg tgatgaccga
     8641 tagccaacga gtaatacatt acctgggagc tgtgttccgg catctcgtcg ctactggcga
     8701 taaatttttt tcgcagcgtc cagaaaacaa tttccccgca ctcttcagtc atgtaacccc
     8761 cggagtacgc gaaagagttg ctgaacgata ctattgagac gtggatcgtc ggcatcgcgc
     8821 aaccaggtgt taacccgatt tcctgtagga tcgtcgctta aatggcgcag gagccgatcg
     8881 caaatttctc gcccctgacg atagcccgcc agccgccgcg cttctcgctc aatggcgata
     8941 cgctgtgatg gtgggatctg cggccacaac ggttgcatag tcaccccggt gggatcgcga
     9001 taatccaccg cgtgaatctt ctgttgcagc aaaccgagcg ccacggcgaa accgtgaatg
     9061 gtggcggccg gtgttggcgg gcagccgggg atccaaacat caatggggac aatggtgtcg
     9121 ctgccgcccc agacgctgta gagatcgtgg aaaataccgc cgccgacacc gcacgcgccg
     9181 taggaaacac agattttatg atcgggggca gactcatacg cccgaagtgc aggcatacgc
     9241 atcgcccggg tgactgcgcc agtaaataac aaaatatcgg cgtgacgcgg tgatgaaaca
     9301 accttaatgc caaaacgttc tgcgtcgaat acgggtgtga tggcggcaaa aatttcgatt
     9361 tcacaggcgt tgcagccgcc gcaatcgacg cgataaacgt aagccgagcg atggatatcc
     9421 tgtagcaaat gccgcttcat cttttgcgtt tgctcgtcca gcgtgatggg ctggctgaca
     9481 tgttgtgtaa gcactggact catagctgct ccttagccac cagcggtaca tctgtatcgt
     9541 cgttgatcag cgtcgcgcgt tgtttgcact ccgggcagac gctcgcttgc gcccgcaaca
     9601 tttcgcggtt ttgtggcgca ttttgttgct gtgccaacaa ttcagcagcc agtgcgacgg
     9661 ttttttgcgg ggcaaacggg cgttcgcaac ggctgcaacg ttgtagatgg aacgtcgcgc
     9721 gggtatagag atcggcttta ttggtgacgg tcagttcaaa gttattggta agctggatgg
     9781 ctctggtcgg gcacacttct tcacaacgtc cgcagtaaat acaacgcccc agatagagct
     9841 gccaggtgcg cgaattttgc tggtcgtcgg tctggatagt cagcgcattt gccggacaag
     9901 cacaggcgca ggcaccacag gcaatacatt ggctgggcat caggtccggt ttcccgcgaa
     9961 agcctgggct gacctccagt ggcgcgaagg gatatttcac cgtcgcggtt ccggcgcgca
    10021 taatagtttt cagtaacttc agcatcttct gtccttactt cagcggcgaa cggttacgat
    10081 caatgccgta gcgttcgatc tctttatacg gcacggtttt tgactggcgc ttgcgcacat
    10141 caaccagcgt cacacggtcg gtacaggagt agcagggatc aaggctaccg ataatcagcg
    10201 gtgcgtcaga aacggtattg ccgcgtagca tgtaacgcag caccggccag ttggcgtagg
    10261 tggcggcacg acagcgccag cggaacaatt tttgattatc accgagcatg ctccagtgca
    10321 cgtcttcacc gcgtggcgct tcaacaaagc ccagcgcgaa tgcgtgaggt ttatagctaa
    10381 agccttcggt cagcagtggg gtatccggca tgttgtcgag agcaaattcc agcattgcca
    10441 gcgaatcaaa cgtctctttg acacggacca tcacgcggga gaaaacatcg ccgccggtga
    10501 aggtaaacag tgtttttgga atattgccgt agtcggcgta ggggtgatca aagcgcaaat
    10561 cacgggcaaa accactgccg cggatcagcg gccctacagg gctataatca cgggcgattt
    10621 gtcggtcgag aatgccaatg ccctgagtgc gttgttccat attcggcgtg gcaagcagca
    10681 tttctaccag ctccgacacg tcggcgcgca tctcgcgcac cagtttcagc gtttgcagac
    10741 gttgctcttt gagaatatcg cggcgaacac caccaatcag attcagaccg taggttttac
    10801 gcgacccggt cagcaattcc gccattgtca tcgacttttc ccgcacgcgg aaaaattgca
    10861 taaagccggt atcaaaacca acaaaatggc aggagaggcc aaggttgagc aaatgactgt
    10921 gtagccgttc gacttccagc agaatcgagc gaatggtatg cgctcgttgc ggcacctcaa
    10981 tcctcagtgc attttcaacc gagttggtat aggccacact gtgggcaaaa ccgcaaatcc
    11041 cacacacgcg gtccgataag aaggtcactt cgttgtagcc catccgcgtt tctgccagtt
    11101 tctccatgcc gcgatggaca taaaacaggc ggtaatcagc atcgacaatt tgctcgccat
    11161 ccacaaacaa gcggaagtga cccggttcat cggaggtgat atgcagcggg ccgacaggga
    11221 ttacccgcgc gtcgctgtcg ccctcgttga taaacggata cgtttcggta tcagtcgtcg
    11281 gttcagggcg cagtcgataa tccatcgcgt ctttgcgcag cggatgcata tcttccggcc
    11341 agtcatcggg caacaccagg cgacgctgat ccggcaggcc aaccggaatc agcccgtaca
    11401 tatcgcgaat ttctcgctcg ccccagaccg cggcagggac gcgcggtgtg actgacggaa
    11461 actcccgact gtcggcatcg accagtgcct tcaccacgat ccagcatttt tcggcccctt
    11521 ccatagaaag ggcataataa acggcgtaat gaccgttaag tgtccgctcg tcgttgccaa
    11581 acaggaccgg aagccagcca tcatgttgat aataaagata atgtacaacg tcaggcagca
    11641 gattgatttt caccgtaatg gtgacctgtt caggcgtttg tcgctcttca tccagcaccg
    11701 cgccggggaa ctgcgttttc agggcggcga gaatcgcttc gccacgattt gacgatgaat
    11761 taacgttcac tgtgtttctc cgaaacagat gcggtgcctg aaggcaaaaa atcatgccag
    11821 gtgctacgtt gtgcaggcag gtcgtgcgtc cctgagagga ctatagtgga agcgcccgcc
    11881 aggatcctga tgacaggttg tggaatatgc gttcccatcg ccagcatcat gaccagcaga
    11941 atcaccattg gcgaggtggt caaccagccg agatcacccc ggttaacggc ctgcggcggt
    12001 ttcgccatta acacccgcgc agccatccgt accaggcccg ccagcaccag cgttaacagc
    12061 aataacagca ggacgataat cagcagatga ttacgtgcca gtccggcggt aacggtcata
    12121 aattcgctaa gaaaaatgtt gaagggcggc atccctgcca gcgccagcgc accgccgcca
    12181 aacagcacgg cggtaaatgg catgattttg agcatcccac agacgacgtt gagatcgcgc
    12241 gtgccgtact tgagcagtac attgccggaa ccgcagaaca gcagcgtttt tgccagactg
    12301 tggtttaagg tgtgcagcag cgcggcaaaa attcccagcg gcccgccaat gcctagcgcc
    12361 accgcgacca gtcccatgtt ctccacgctg gagtacgcca gcagacgctt aatgtcccgc
    12421 tgtaccagaa tgaaaaatgc cgccacggca accgacaaca tgccgaagat gagcaacaac
    12481 cggttgggga aatcgctgcc gatggcctgg caaataatga tgtaatagcg aatcagcacc
    12541 aacagcgcgc agttcagcaa tacggcggag agtagggcgc tgaccggact cggcgcttca
    12601 ctgtgagcat ccggcagcca ggcgtgcatg ggaaatagcc cggttttggt accaaagcca
    12661 attagcacaa acacaaaggc caacagcatt aatgttggat caagcaagga cgattgctta
    12721 agaacctcgc tccagaatat cgccatttct gcctgcggca taacgctggc ggcgttggcg
    12781 tataccagca cggtaccgaa cagaccaaaa gcgacaccaa cagtacaaat aatgatgtac
    12841 ttccatgctg cttccagcga tgaacgctga ccgtaaatgc ctaccagaaa cgccgagctt
    12901 aaggtggtgg cttcgatcgc cgcccacatc acaatcaggt tattgctggt aacaaccagc
    12961 agcatggtga acaaaaacag atggaagaaa ccgtagtaat cgcacagcgt aacgggtgaa
    13021 agctcgccgt gagccacttc atgacgcatg tagccaatcg agtaaacacc ggtgagaaag
    13081 ccaatcacac caagaatggc gaggaacaaa ccgcccagac catcaatatg aagccacagt
    13141 cccgcagcga atatttctcc tgcatcagcg gcagtttgga ccacccagag tgccagaatc
    13201 agcagcagtg tgatccctaa gcaatgtaat acggtcaccg tgcgagtcgc agagtgcccc
    13261 cgtttccggc aggcaaaaca gagcagcgaa aaaagcagcg gcgtgagcag gagtaaagcg
    13321 aacatcacag aataactcat ctcattatcc cttcagcgcg gtcaagttgt tcacgtccag
    13381 cgtgccgtgg gtacgccata tttttcttgc cagtaacacc atcacaatga cggcgaagat
    13441 ggcgtcggta gcgataccta tttccaccag ttccggtgca cgccaggcaa gaagcgccag
    13501 caccagatgg gagccgtttt ccatcaggca gtaaccaaaa atttgccgca ggatattgcg
    13561 ctggctgaca atgcacagca ggccaagcag aaaatgacct aacgccaccg ccagcgctgg
    13621 tttcagcccg gtagccatcg gtagcttcac gggctgaacg acaaatgcgc aaagcaggac
    13681 aattaacgcc gcgagcagtg ccatcattgc cggaccgaat aacgcttttt ccgggatgtt
    13741 ctggggaata tttcgtgcag cgtaagtcat gattaacggt accagcagca ctttggtgat
    13801 aaaggcgctg gcggaccaga tcagcagttg ctctgcggcg aacaggcacg agagggtggc
    13861 gaaaatagac accagcacca gtgactggca ggcgtaaaat ccgcatgaca ggcgatagct
    13921 tttgacgcta atcacaaaca gcgatgtcag catcatcagt cccgccagat tatttacgat
    13981 cgtagaaccg gtcattcatc atctccttac tgcaaccagg tcgcggcgat tacgctggaa
    14041 atcagcgaca tcacaatcag tacaatcagc accaggcgca ttgaccctgg cagcggtgcg
    14101 gcatcggcga cggcctcgct cggtttgccg gggacgacgc gaccaaacca gcgaataaac
    14161 caggcgaaac tggcgaccga ttcaatcatc agcagaatca tggcgggcag caggatccag
    14221 tactccactg acaacgcaaa accggcagca aacagcggga atttactgaa gaagccgttg
    14281 aacggcggta cgccggtaat cgccagcgct gccacgcaga aacccacgcc tggcaacggc
    14341 agggtgtgca atacgccacg cagacgcggc aacaagcgcg tgccgcagct gtaactcagc
    14401 gcacctgcta caaggaaaaa caggctttta gcgaacgcgt ggttgacgat gtaggcgatg
    14461 ctaccctcca gcgccagccg cgagccgaag atggagagcg acaagccgaa gaacatccag
    14521 ccaagttgag tgatggtcga ccaggccagc aaccgcttca tatcctgctg tggcaaatac
    14581 atcagaaatc cataaagaat ggtgaccagg gccatgacca tgccaacacc gccaatcaca
    14641 tgcgggatat tgccgccgtc gataatggcg cgggcaaaaa tgtaaacgcc cactttcacc
    14701 atcgatgcgg cgtggagata ggcgctgatc ggtgttggcg cttccattgc gtccggtagc
    14761 cacgcttgca tcggtagctg ggccgatttc ccccacgcgg caaacaggat gccgccataa
    14821 accagataac gtgcgtcgcc gtgtaactcg ctcatcgcgc taagcgcaaa cgttccggtc
    14881 tgcaaaaaca gcgtggcggc ggcaagatac aaccccaacg agccgatatg agtgataagt
    14941 aacgctttta gtgctgaacg ctgtgattta tcgctctggt aataactgat caacgcccag
    15001 gagcagccgc ccgtaatttc aaaaaacaac aactgaccga gcagcgtcga ggagagtacc
    15061 agtcccgcca tcgcgccgat aaacaacagc aaaaatgcgt aataacgatt cgtgccgtta
    15121 tgcgggtgtt cgcgattttt atccgtcaga taacccgtgg agtagatcgt gaccagcaaa
    15181 ccgagaaaca ccaccacaaa cagaatcagc gtactcacgc gatcaatgac caggccaaac
    15241 aatgccacat cccccacgtt gaccaacgtc aacgtgacgg caactttatc ggcctgataa
    15301 aacgcggaga tcagcgacaa catgcacagc gtggtcagcg cggcgaacaa aaccccccat
    15361 tcggcggccc gacgttgtgg cgaaaacgaa acgaccagtg cgccgataaa aggcagcatt
    15421 aacgtcgtca gagcaagatt ttccatattc cgtcagtgct ccttacagac cggttaacca
    15481 gaagacccat gcaagcgcag caaggctgaa gccaagccag gtcacatggt gaatgagtaa
    15541 aaaacgcccg cgtgccagcg tgttttctgc gatagaggcc agtacaaaaa tcagcaaaac
    15601 cttgagcagc gtaacgacaa gtgaagtcag caggcaggcg agagaaagtt cttgcgcgcg
    15661 cccaaagggc agaaacaggg ccacaaacag tgctgccatc acgacctgtt tcagccccag
    15721 cccccatttc gccagcgcca gcccggcacc ggaatattct gtcagcgggc cttcctgtac
    15781 ttcctgttct gcttcagcaa catcaaaggg aatttttccc atctcaatga agcaggcaaa
    15841 accacaggcc agtaacgcca gtacggtggt tagcggcgag ttccagccca tcgccagcgt
    15901 attgctgatc atctcgatat gcgtggaatc cgctatcagt gccaatacca gcagtgagag
    15961 aatgagcatg ggttcgacca gaatgccgag cgtcaactcg cgactggcac cgactcccgc
    16021 aaacgggctt ccggtatcca gcccggaaag agcaaagaaa aaacgaaaca gggcaagaag
    16081 ataaataagg gtgatcagat cgccgccgcc cgcaaaaggg gaaacggcaa taaatagtgg
    16141 tagggccatc gacagcacca gcatgctgct gattaatacc cacggcatca ggcggaacat
    16201 cagacctgaa gatgtcggcg caacttcctg ccgtttaaac agtttgtgga tatcgcgata
    16261 atcctgccag atccccggtc cgcggcggga gtgcattcgc gcgcgtatct gccgggaaat
    16321 acccgtaaaa agtggggtta gcatcagcag aatcacggcc tgtgctaacg caaaaatgac
    16381 cagatatccg tcgcaaagag tttgtctcat ggtgatttcc ttagacggca atagcgatta
    16441 gcagcacaac cagtgcggcg accacataca ggcaatagag acgaaagtcg ccgctttgca
    16501 gatgctgtat ttctttggcc agccgttggg tggcgctcac gatggggcgg atcacccgct
    16561 catcccagaa gggttctgtg ctctgagccc tggcggtgac gtgcgcaaga cctttattta
    16621 gcctcagcgt agggtcgagc tgttgtcgta gacgaaataa cgcagagaag accacacgca
    16681 gcggctgcat cacgccattg cctgacggag ccatcgcgtt ttccctgctg tagccgcatg
    16741 cccaggcatc tcctgtgcga cgaaacgctg cgcggcgcga acgacaccac agccagtaaa
    16801 ggccaggcag caaaggcatc gccagtagta acagcaacag taatgagggg gtgacctgtg
    16861 tatgaaacgt gccgggtaca agtgctattc cgctggcgac agtgacggga ggggtattgg
    16921 taaacgcatg ggcaatatgc attatcttcg gtgccagcca actggcacta agcgcaatta
    16981 atacgcagag tgccgcgagc agtagcatgg cgacgatcat cgtatttggc acttcctgtg
    17041 cctcttcagc gtgtgtactg cgcggcgcac cacagaaagt aataccgtac atttttacga
    17101 agcacattac tgccagccca ccagtgactg ccagcattac catagcaata ggacccgcaa
    17161 gttgtagcgc tacggcttcc acacgactta gtgagaacag cgactgccag gtgtaccatt
    17221 cgctgataaa accattcagc ggaggaaggg ctgatatcgc gaggcaacca atcaggcatg
    17281 ctgcggctgt ccacggcatc cgtttcgcca gtgcccccat tttttccatg tcgtgggtat
    17341 gcaaacgcga aataatcgcg cccgcgccga gaaatagcag ccctttgaac agcgcatggt
    17401 tgagcagatg aaacagtgcg ccgagcagtc caaccacggt gagcagcggg tcgtgcagtg
    17461 acagaccgac catcgccaca ccgaccgcca gcaaaataat gctgacgttt tcgacggtac
    17521 tccaggccag cagccgtttg atatcctgtt ccgccagcgc atacagcacg cccaggagcg
    17581 cggagattgc gccgatcgcc atcaccagaa tgccccacca cagcggcaaa cccgtttgcg
    17641 ccagcagatc catcgccact ttcaggatgc cgaaaatacc gattttgacc attacgccag
    17701 acatcaacgc cgaagcgtgc gatggtgcgg cagggtgagc gcgcggcaac cagctgtgca
    17761 acggcatcat cccggctttc gcgccaaaac cgaaaaaggc cagcaggaac accgccgacg
    17821 ccagccccgg agaaagtgaa agcgtgcgga aactggcaaa atcgaggctg ccgctttcgc
    17881 gccccatcag caagaaggcg atcataatca gcaccgatcc ggcgtgggcg ataaaaaagt
    17941 agagcatgcc agcgttgatt gacgttttat cctgcctggc aatgaccaga aaccaggaag
    18001 acagtgacat catttcaaac agcacgatga accaaaaagc gttgtccatc accagcaggg
    18061 caaccatcga tgcgatgaaa atattcataa agaagcccat cgccgccgcg cctttgccct
    18121 cgtattcgcg catataagtc aatgaataga gcgaacaaac caccaccagc aaggagataa
    18181 ccagcaccat aaatgccgag aggctatcca ttcgcagggt taaatcggca aagtcaaaag
    18241 gggtggcaaa acgggcgact aaagttacgc ctgcatgtaa ttgcgtaatt ccgctgataa
    18301 tgccaatcac gccgcccacc agcgatgtga tgccggaaag cttaatagcc agtctgtcca
    18361 gaccgagtaa aaacagcgaa gccagactgg caaacagata gaggatcagc gaccaggtta
    18421 ataattgcag ggcatccatt agcgttgctc ctgagtgagg gaaaggggat cctccccgcc
    18481 attggcaaac cagcttgcgg caaggcgctg tttttctttc atctgacgtt gcaggctatc
    18541 gtcggtgatc agccgtaacg cctgattcgg gcaagcgcga acgcaggccg gaccttgtgg
    18601 caagaaatca cacaggtcgc atttcaccgc gacggtctgg acacccggtt cccagcgcag
    18661 caaagcatgt tgggttggcg cgttttcttc gccgtctttg agtgagcctt cagcctgaaa
    18721 aacatattgc gcaggggcat tcaccggacg gcttcctgaa gcgctgattg cgccaaatgg
    18781 gcagaccacg gcgcaaagct tgcagccaat acagaggctt tcgttgagtt ggatcgcatc
    18841 atccctctgg gagatggcat tgaccgggca gacctgcagg cacggggctt cctcacagtg
    18901 atgacacacg acaggggcag tgagtgttga cgtcttcgcc agggccaggc gcgggtgttg
    18961 ctgtaaacct tgcgttttat gcacgtccga acaggcagcg agacaggtat tacatcctgt
    19021 acaccacagt ggttcggcca ccacaaagcg gttcatgtcg gctccttgaa aatgatatga
    19081 aacattgcat ttcatattgc ataaaggatg ccagtctgat taatttaata tattcaataa
    19141 gttatgaatt aactggattc tgaacggtac aaaaatgatg gtcattaacg ttcagcaaaa
    19201 aatggattcg acacccgtgt cgcaggggaa agtgagaatg tgatcttgat cacgataagt
    19261 gaggtgtgct gaccgcaagc gcagccagca cggaatggag caaagtaatc aggcgtgttc
    19321 tttcagccag ttcagcacaa cgtcgtggtg attgctggtt ttgaaatcgt caaagacatg
    19381 ttcgattttg ccatcagcgt caatcaggaa gctgatgcga tgaataccat cgtaggtttt
    19441 gcccatgaag gacttttccc cccagacgcc gaattgctcg cacacatggt ggtcctcatc
    19501 agacaggagc gtaaagttaa gcagctcttt ttccgcaaaa cgggagagtt tttcgggttt
    19561 atcggtgctg atacccagca catcaacgcc cgcttttttc aactcatcca tgttatcgcg
    19621 taagccgcag gcctgtacgg tacagccggg ggtcatggct ttcgggtaga aataaaccag
    19681 aacacgctgt ccctggaagt cggtcaaatt aacttgttct ccgtcttgat ccggcaagct
    19741 aaatttcggt gcgatatcac cggctttcag tggattcatt acttaactcc atcctgttca
    19801 tcatgttggg aataattgac gacgttaata ctgccttgtg cattgagttc tgtacatagg
    19861 gctttgaacg cttgctcaat atttgctgcg tccgcagatg cggggctgtg ggcagtaatc
    19921 tgaatatgca actgcgcagc ccgttcattt tcagcaggtt gtgtgcgcga caccagctcc
    19981 gcaatgttca tatgatgcgc gtcgaaaagt gctgtgaagc gttcaattaa atgcggggag
    20041 tctgccacat cgacctgaac ccagacagat gctggcattg gcggacgcgg acgcgccgtc
    20101 gtgcgcttca tcacgattaa aagatccagt tcggcacctt tcaacggtaa cgttgattca
    20161 atcagagtaa tggcattcca tgaaccggaa agcagcatga taaacgtgaa ctcttctccc
    20221 agcatcgcca ggcgactgtc ttcaatatta cagccgcaac tactgacatg acgggtgatg
    20281 gtgttcacaa ttccagggcg atcggcaccc aacgcagtga tcaccagata atgttgcgat
    20341 gacagtgtca aactggttat tcctttaagg ggtgagttgt tcttaaggaa agcataaaaa
    20401 aaacatgcat acaacaatca gaacggttct gtctgcttgc ttttaatgcc ataccaaacg
    20461 taccattgag acacttgttt gcacagagga tggcccatgt tcacgggaag tattgtcgcg
    20521 attgttactc cgatggatga aaaaggtaat gtctgtcggg ctagcttgaa aaaactgatt
    20581 gattatcatg tcgccagcgg tacttcggcg atcgtttctg ttggcaccac tggcgagtcc
    20641 gctaccttaa atcatgacga acatgctgat gtggtgatga tgacgctgga gctggctgac
    20701 gggcgcattc cggtgattgc cgggaccggt gctaacgcta ctgcggaagc cattagcctg
    20761 acgcagcgct tcaatgacag tggtatcgtc ggctgcctga cggtaacccc ttactacaat
    20821 cgtccgtcgc aagaaggttt gtatcagcat ttcaaagcca tcgctgagca tactgacctg
    20881 ccgcaaattc tgtataatgt gccgtcccgt actggctgcg atctgctccc ggaaacggtg
    20941 ggccgtctgg cgaaagtaaa aaatattatc ggaatcaaag aggcaacagg gaacttaacg
    21001 cgtgtaaacc agatcaaaga gctggtttca gatgattttg ttctgctgag cggcgatgat
    21061 gcgagcgcgc tggacttcat gcaattaggc ggtcatgggg ttatttccgt tacggctaac
    21121 gtcgcagcgc gtgatatggc ccagatgtgc aaactggcag cagaagggca ttttgccgag
    21181 gcacgcgtta ttaatcagcg tctgatgcca ttacacaaca aactatttgt cgaacccaat
    21241 ccaatcccgg tgaaatgggc atgtaaggaa ctgggtcttg tggcgaccga tacgctgcgc
    21301 ctgccaatga caccaatcac cgacagtggt cgtgagacgg tcagagcggc gcttaagcat
    21361 gccggtttgc tgtaaagttt agggagattt gatggcttac tctgttcaaa agtcgcgcct
    21421 ggcaaaggtt gcgggtgttt cgcttgtttt attactcgct gcctgtagtt ctgactcacg
    21481 ctataagcgt caggtcagtg gtgatgaagc ctacctggaa gcggcaccgc ttgcggagct
    21541 tcatgccccg gctggaatga ttttgccggt gacctccggt gattatgcaa tcccggtgac
    21601 caacggtagt ggtgctgtcg gtaaggcgct ggacattcgt ccaccagccc agccgctggc
    21661 actagtttct ggcgcgcgta cccagttcac gggcgatacc gcttcattgc tggtggaaaa
    21721 tggtcgtggc aatactctgt ggccgcaggt ggttagcgtg ctgcaggcga aaaactacac
    21781 catcacccaa cgtgatgatg ctggtcagac actgaccacc gattgggtac aatggaaccg
    21841 tctggacgaa gacgagcagt atcgtggtcg ttatcaaatc tctgttaagc cgcagggtta
    21901 tcagcaggcg gttacggtta aactgctgaa cctggaacag gcgggcaaac cggttgcaga
    21961 cgcggcttcc atgcagcgtt acagcacgga gatgatgaac gttatttccg ccggtctgga
    22021 taaatctgcc actgacgccg cgaacgctgc gcaaaatcgt gcctccacca ctatggacgt
    22081 acaaagtgca gctgatgaca ccggtttacc aatgctggtc gtacgcgggc cgttcaatgt
    22141 ggtttggcaa cgtctgccag cggcgctgga aaaagtgggc atgaaagtga ccgacagcac
    22201 ccgttcgcag ggcaacatgg ccgtaactta taagccgctg tctgacagcg actggcagga
    22261 actgggcgcg agcgatccag gcctggcatc cggtgactat aaactgcagg ttggcgattt
    22321 agataaccgc agcagcctac agttcatcga tccgaaaggt catactctga ctcagagtca
    22381 gaacgacgcg ctggtagctg tcttccaggc tgcgtttagc aagtaaaaat acagggctgg
    22441 aatcatccgg cccttttttc tgatatgata cgcaaacgtg tgcgtcggca ggaaaacgcg
    22501 attttagcgg taattcgcac gaaatttgtt tgtcggacgt agttcggata aggcgttcac
    22561 gccgcatccg acaaaacatc cggcacacca gacagcaaaa gattttaaaa cgttaattca
    22621 cacccaggag tgataaagat gcaaaagcaa gctgagttgt atcgtggtaa agcgaaaacc
    22681 gtatacagca cggaaaaccc ggacctgttg gtgctcgaat tccgcaatga tacgtcagca
    22741 ggggatggcg cgcgcattga gcagtttgat cgcaaaggta tggtgaacaa caagttcaac
    22801 tacttcatta tgagcaaact ggctgaagcg ggtatcccga ctcaaatgga gcgtctgctc
    22861 tctgataccg aatgtctggt gaaaaagctg gatatggtac cggttgagtg tgtcgtgcgt
    22921 aaccgtgctg cgggctctct ggtgaaacgt cttggaatcg aagaaggtat tgagctgaac
    22981 ccgccgctgt tcgatctgtt cctgaaaaac gacgccatgc acgatccgat ggtcaacgaa
    23041 tcttactgcg aaacctttgg ctgggtgagc aaagagaacc tggcgcgtat gaaagagctg
    23101 acctacaaag cgaacgacgt gctgaaaaaa ctgttcgatg atgctggtct gattctggtc
    23161 gacttcaagc tggaatttgg tctgtacaaa ggcgaagtgg tactgggtga tgagttctcc
    23221 ccggacggta gccgcctgtg ggacaaagaa acgctggaga aaatggacaa agaccgtttc
    23281 cgccagagcc tcggtggcct gatcgaggcc tatgaagccg tcgcccgccg cctgggtgta
    23341 cagctggact gatttttctg ttcatcatct tgccgtgctg ctggcacggc aagacaacca
    23401 ttctcgtaag atgtgcattg aacgctcatt ccgccgccat ttcatcccgc tttcctgtgg
    23461 taatcttcct ctgaaccacc tacgatcatt tctataatga atatattgtt gaggtatcta
    23521 tgcgttggca agggcgacgt gaaagtgaca atgttgaaga caggcgcaat agctccggcg
    23581 gtccgtcaat gggcggtccc ggtttccgtc tgccgagcgg taaaggcggg ctgattttac
    23641 tgatagtcgt gctggttgca ggctactatg gcgttgattt aaccgggttg atgaccgggc
    23701 agccggtttc tcaacagcaa tcaacgcggt caattagccc aaatgaagac gaagccgcaa
    23761 aattcacctc ggtgattctg gcaaccacgg aagacacctg gggacaacag ttcgagaaga
    23821 tgggtaagac ctatcagcaa ccgaagctgg tcatgtaccg tggaatgacg cgtaccggat
    23881 gcggggcggg ccagtccata atggggccgt tctattgccc ggcggatggc acggtttata
    23941 tcgatctctc cttctatgat gacatgaaag acaaacttgg cgcggatggc gattttgccc
    24001 aggggtacgt tatcgcccat gaagtcggtc atcatgtgca gaaactgtta ggcatcgagc
    24061 cgaaagttcg tcaactgcaa caaaacgcga cgcaggcgga agtgaaccgc ttatctgtgc
    24121 gtatggaact ccaggccgac tgttttgccg gtgtctgggg gcatagtatg cagcagcaag
    24181 gcgttctgga aaccggcgat ctggaagagg cgctgaacgc ggcgcaggcc atcggcgatg
    24241 accgtttaca acagcaaagt caggggcgag tagtaccaga cagtttcact catggcactt
    24301 ctcagcaacg ctacagctgg tttaaacgtg gtttcgacag cggcgatccg gcacaatgca
    24361 atacttttgg taaaagcatt taacttccgg ggcagggatg gctgaactga ctgcgcttca
    24421 cacattaaca gcgcaaatga aacgtgaagg gatccgccgc ttgctggtgt tgagcgggga
    24481 agagggttgg tgttttgatc atgcgcttaa gttacgtgat gccttacctg gcgactggct
    24541 gtggatttcg ccgcagccag atgctgaaaa ccactgttct ccctcggcac tacaaacttt
    24601 acttgggcgc gagttccggc atgcggtatt cgacgcccgc cacggctttg atgccgctgc
    24661 ctttgccgca cttagcggaa cgttgaaagc gggaagctgg ctggttttgt tactccctgt
    24721 atgggaagag tgggaaaacc aacctgatgc cgactcgctg cgctggagtg attgccctga
    24781 ccctattgcg acgccgcatt ttgtccagca tttcaaacgc gtacttacgg cggataacga
    24841 cgctatcctc tggcggcaaa accagccgtt ctcgttggcg cattttactc cccgtactga
    24901 ctggcacccc gcgaccggcg caccacaacc agaacaacag caaatcttac agcagctact
    24961 gaccatgccg tcgggcgtgg cagcggtaac tgctgcgcgt gggcgcggta aatcggcgct
    25021 ggcagggcaa ctcatttccc gtattgcggg tagtgcgatt gtcactgcgc ccgcaaaagc
    25081 ggcaacggat gtactggcac aatttgcggg cgagaagttt cgctttattg cgccggatgc
    25141 cttgttagcc agcgatgagc aagccgactg gctggtggtc gacgaagccg cagccatacc
    25201 tgcgccgttg ttgcatcaac tggtatcgcg ttttcctcga acgttgttaa ccactacggt
    25261 gcagggctac gaaggcaccg gacgtggttt tttgctgaaa ttttgcgctc gctttccgca
    25321 tttacaccgt tttgaactgc aacagccgat ccgctgggca cagggatgcc cgctggaaaa
    25381 aatggttagt aatgcactgg tttttgacga tgaaaacttc acccatacac cacaaggcaa
    25441 tattgtcatt tccgcatttg aacagacgtt atggcgaatc gagccagaaa cgccgttaaa
    25501 ggtttatcag ttattgtctg gtgcgcacta ccggacttcg ccgctggatt tacgccgcat
    25561 gatggatgca ccagggcaac attttttaca ggcggctggc gaaaacgaga ttgccggagc
    25621 gctgtggctg gtggatgagg gtggattatc tcaagaactc agtcaggcgg tatgggcagg
    25681 ttttcgtcgc ccgcggggta atctggtggc ccagtcgctg gcggcgcacg gcagcaatcc
    25741 actggcggcg acattgcgtg gacggcgggt cagccggata gcagttcatc cggctcgtca
    25801 gcgggaaggc acagggcggc aacttattgc tggtgctttg caatatacgc aagacctcga
    25861 ctatctttcg gtaagttttg gttacaccgg ggagttatgg cgtttctggc aacgctgcgg
    25921 ttttgtgctg gtgcgaatgg gtaatcatcg tgaagccagc agcggttgct atacggcgat
    25981 ggcgctgtta ccgatgagtg atgcgggtaa acagctggct gaacgtgagc attaccgttt
    26041 acgtcgcgat gcgcaagctc tcgcgcagtg gaatggcgaa acactccctg ttgatccact
    26101 aaacaatgcc gtcctttctg acgacgactg gcttgaactg gccggttttg ctttcgctca
    26161 tcgtccgcta ttaacgtcgt taggttgctt attgcgtctg ctacaaacca gtgaactggc
    26221 attaccggcg ctgcgtgggc gtttacagaa aaacgtcagc gacgcgcagt tatgtaccac
    26281 acttaaactt tcaggccgca agatgttact ggtccgtcag cgggaagagg ccgcgcatgc
    26341 gctgttcgca cttaatgatg ttcgcactga gcgtctgcgc gatcgcataa cgcaatggca
    26401 attttttcac tgactccttc aattttctcc catggtcatt gtgagtcagc ggcgtaaaat
    26461 taatgacatc agttaaggag ttcgccatga aacatgacca ttttgttgtt caaagcccgg
    26521 ataaacctgc gcaacagttg ttactgcttt tccacggtgt cggggataac ccggtggcaa
    26581 tgggggaaat aggcagctgg tttgcaccgc tgtttcctga tgcgctggtg gtgagtgtcg
    26641 gtggcgcgga gccgagcggt aatccggcgg ggcgtcagtg gttttcagtg cagggtatta
    26701 cggaagataa tcgccaggcg cgtgtggatg cgatcatgcc gacgtttatt gaaacggtgc
    26761 gctactggca gaaacagagc ggagtagggg ctaacgccac ggcgctaatc ggtttctctc
    26821 aaggcgcgat tatggcgctg gagagcatta aagccgaacc gggccttgct tcgcgcgtca
    26881 ttgcctttaa cggacgctat gcgagcctgc cggaaaccgc gtctacagca acgacaattc
    26941 acctgattca tggcggtgaa gatccggtta tcgatctggc gcatgcggtt gcggcgcagg
    27001 aagcgttaat tagtgccggt ggtgacgtga cgctggatat cgtggaggat ttaggccatg
    27061 cgatcgacaa tcgcagtatg caatttgccc tggatcactt gcgttatacc attccgaaac
    27121 attatttcga cgaagcgcta agcggcggta agcctggcga tgatgatgtg attgaaatga
    27181 tgtaataata gccgggtagg cctgataaga ccgtatcagg catcatggcc ggatgcggcg
    27241 tgaacgcctt atccggccac tcagcttact tcttcggttg atcctttttc ggccagtcgt
    27301 cgtcatcgtc ccacttatcg ttgaaatcac gatgcggggg aagctccggc ttgttggcga
    27361 gaaatttctt atggtcgacg cgcttgagat ctttaatcac gttcagcagg acgcctacca
    27421 aaaagacaat caccagaatc caccaatatt tagccagcca gtccatgctt atttcctctt
    27481 gcagaaccac tcatcacgcg acgagctgtt ccatgatacg ttgatacata cgggcaagta
    27541 gctgcaggtc ggcagcgttc acacattcat taattttatg aatagtggca ttgaccggcc
    27601 cgagttccac cacctgcgcc cccatgcgag caataaagcg cccgtcggac gttccgcctg
    27661 tggtcagtag ctgcggttta atttcattat agtgctcaac cgcgttaacg accgcatcca
    27721 ccagtttacc gcgcgcggtc aaaaatggct gcccggaaag ccaccaatcc accgtatagc
    27781 gcagttgatg tttttcaagc agggcaagca cctgcgcttt gatcatctca tcagtcagtt
    27841 cggtgctgaa gcggaagtta aactgcacaa acagttcacc cggaataacg ttgttactgc
    27901 ccgttcccgc ctgaatattg gcaatctgca tactggtcgc cgggaagaat tcattgcctt
    27961 gatcccactc aatatccact aattcattaa ggaaaggtgc tgcgcgatgt accggattgt
    28021 cagccagatg tgggtaggca acatgcccct gaacgccatg aatggttagg ttgcaggtta
    28081 acgatccacg acgaccattt ttcaccacat cacctaccac ttcgatactc gacggttcgc
    28141 cgaccaggca gtaatcgaga cgctcattac gtgccattaa cgcttcgacg acttttaccg
    28201 taccattgtg tgcactggct tcttcatcag aggtgatcag aaatgccagt cgccccgtat
    28261 ggttgggatg ttgtgcgaca aaacgttctg ctgctaccac cattgccgcc agtgagcctt
    28321 tcatatctgc cgcaccgcgc ccgaataaca tgccgtcacg aatggtcggt tcaaacggcg
    28381 gattgatcca acggtcggcg tcgccaggcg gcaccacgtc ggtatgcccg gcaaaggcca
    28441 acgtttcacc ttgcccacgc catgcccaaa aattctgcgt atcggcaaaa tccatgcgtt
    28501 caacggtaaa accgatcgcc tgcaaacgtt caatcaacaa agcctggcat cctgcgtcat
    28561 caggactcag ggaagggcgg cgaataagct gttgtgtcag ctcaataacc gggcacgaca
    28621 tagactacac ctcatggaaa aattgctgat aactggaatc actgaaaccc agcagcatag
    28681 gcttaccggg cgcgcagagc aatggacgtt tgataattgc aggcatttca gtcattaatg
    28741 ccgccgcaga ggccgcatcg gtgattttat tgcgggtggt ttcgtccagt ttacgccagg
    28801 ttgtaccacg ggtgttgagt aacgcttccc agcctaattc gttgataaaa ccgttcaata
    28861 attcgctgtc cagcccatcg acgcggtaat catgaaaacg atagtcgatg ttattggttt
    28921 ccagccaacg gcgagccttt ttaatggtgt cacaattttt gatgccgtaa agtgtaacca
    28981 taatgaatcc tttttcgatt tatgagaata aatactcatt taagagtgaa aacattttgc
    29041 ctgcaaatga ttatcaggaa atataacatt atcgaagtat tcaaattaac aactattaaa
    29101 taatattaaa catttttaac gtaaacatta agaatattca tcttgttatc tgtctgtatt
    29161 ttaaccagaa ttaaatcagt attagatgcc gtatgaatat ctcgaaatgt tgctgtgtat
    29221 cacatacaaa tcatcaccgc aggaccatag tatttacata aggtttgtcc cccggaggta
    29281 gtcatcgcag caatcgagta aattagcttc accacggtat attgtttccg tagaataccc
    29341 ataattataa gagaggttgt tatgattgaa cgtgaactgg ggaactggaa agactttatc
    29401 gaagttatgc ttcgtaagta attcctgaga caaagtacaa acagcaagaa cccgctgagc
    29461 aggttcttaa tcggaaagtt gaaaaaggcg acgcattagc atgtcgcctt ttttattgct
    29521 tattccgggc gcggcttcag cgggaagcgg cggcgcacca gcacaaagaa cagcggcacg
    29581 aagtaaatag ccagaatagt agctgaaatc atcccgccca ttacgccagt acccaccgca
    29641 tgctgaccac cggaaccggc acccgtgctg gttgccattg gcaatacgcc gaagataaat
    29701 gccagcgagg tcatcagaat cgggcgtaaa cgctgacggc aggcgtggag cgttgcttca
    29761 aacaggtcgt ggcctttttg gttcatctcg ttagcaaact cgacgatcag gatggcgttt
    29821 ttcgccgata aaccaatgac cgttaacagg cccacctgga agtaaacgtc gttttccagc
    29881 ccgcgcatcc aggttgccag cagcgcgccg attaccccca gcggcacgac cagcattacc
    29941 gagaacggta ccgaccagct ttcatacaat gcagccagac acaggaatac caccagcaag
    30001 gaaatggcgt acagcgccgg agcctgcgcg ccggaaagcc gctcctgata cgacatcgcc
    30061 gtccactcca gaccaaagcc gtttggcagc tgcttcacta acgattccat aatatccatc
    30121 gccgtaccgg tactgacccc cggtgcggct tccccaacaa tttcaaccgc agaataaccg
    30181 ttatagcgtt ccagacgcgg cgagcctgtc tcccagcgtg aggtcgcgaa agcagagaag
    30241 ggcaccatgc cgccatcttt atttcggaca taccagagat tgatgtcatc tggcagcatg
    30301 cgatacggcg cagctgcctg cacatagact ttcttcacgc gaccgcgatc cataaagtca
    30361 ttcacatagc tcgaacccca ggcggtttgc agtgtgtcgt taatatcgtc gatagcaacg
    30421 cccagcgcct gagctttacg ctggtcgata tcaatctgca actgcggact gtcgtcgagg
    30481 ccgttatggc gcacacgggt tagcttcggg ttttccgccg ccagcgccag caactggtta
    30541 cgtgctgcca tcagcgcatc gtgacctgct ccagcgtggt cctgcaactc catatcaaaa
    30601 cccgcagaac tgccaagacc gctaattgct ggcgggctgc tggcgataac gcgagcttct
    30661 ttaatcttgt taaacgcctt cgttgcacgc tcgataatgg caaacgaggt gccggtctta
    30721 ctgtcgcgtt cgctccagtc tttcagacgg ataaacattc gcgccacgtt ttgcccgttg
    30781 cccccaggac cagagccaac ggtggcaaac accgacatga tgttgtcttt ttcatgggtg
    30841 aagtaatatt tctcgatttg ctcaacgact ttcagggtct gttgttgggt tgaaccgctg
    30901 ggcaattgta ccgaggtagt aaacatgcca cggtcttcca gcggtaagaa cgacgtcggc
    30961 aaacgcagga acaggaacac catgccgcca agcagcagga cataaatcac aatccagcgc
    31021 aggctacggt ggagaatttt cgccacccct ttttcgtagc gttcggcgtt gcggttaaac
    31081 atctggttaa accaggcaaa aaagcctttt tgcccgtgat gctcaccttt tttcagtggc
    31141 ttaagtagtg tggcacacag agccggagtg aggatcatcg ctaccagtac tgacagcacc
    31201 atcgccgcaa caatggtaat agagaactgg cgatagatgg caccggtggt gccgccgaag
    31261 aaggccattg gtacaaatac cgccgaaagc accatcgcaa tcccgaccag tgccccctgg
    31321 atctgcccca tcgatttacg tgtggcttcg cgaggagtga gtccttcctc actcataata
    31381 cgttcgacgt tttccaccac cacgatggcg tcatccacca gcagaccgat agccagtacc
    31441 atcgcaaaca tggttaaggt gttgacgctg taaccgaagg cgtaaagtac ggagaaggtt
    31501 cccatcaaca ccaccggcac ggcgatagtg ggtatcagcg tggcgcggaa gttttgcagg
    31561 aacagataca taacgaggaa aaccagggcg atagcttcca gcagcgtttt caccacgtct
    31621 tcaatggagg ctttaacaaa cgaggtggtt tcatacgcca ccttgtattc cagtccgtgc
    31681 gggaagtact gcgccagctc gtcgagacga ttgagcacca gctccgctgt cgccatttcg
    31741 tttgcgccgg aggccagttt tacccccaac ccggaggctg gcttaccatt gaagcggcta
    31801 agataatcgt atttctccgc ccccatttcg acggtggcga catcgcccag ccttacctct
    31861 gagccatcct gattcacccg caaggtgata tcgcggaact gttctggtgt ttgcagcagt
    31921 gactgggcgt taatggtggc gttgagcgcc tgcttatcga cggaaggtgt acccccaagt
    31981 tgcccaaccg caatctgcgc gttctgtgac tcaatggcat cagtgacatc tttagccgtc
    32041 atctggaaac tgttgagttt cgccgggtcc agccagatac gcatggaata ttgcgaacca
    32101 taggcatcga tatccccgac gccattcacg cggcttaacg ggtcctgaat attactggca
    32161 acataatcgg caatatcctg tttatccatc gaaccatcgg tagagacgaa agcaatggtc
    32221 agaatattgg tatcgccggt tttacgcacc gtcacgccct gattttgcac cgcctgcggt
    32281 aactttcgca tggctgattg cagctggttt tgtacttgct gcacggcttc gtccggatcg
    32341 gtgcctgctt taaaacttaa agtgacagat gcctgaccgg tgccactgct ctgagatgac
    32401 atatacatga gattatcgag gccggtcata ttttgctcga taacctgggt cacggtgttt
    32461 tccagcgtct gggccgatgc gccgggatag ttagcggtca ctcgcacatt cggtggcgcg
    32521 agatcggggt attgttcaac gggcaatgaa aaaatcgcca gggtacctgt cagacacaac
    32581 aggattgcca gcacccaggc aaaaatgggg cgatcaataa agaaattcgc cattaaaaga
    32641 ggacctcgtg tttctgcgta tcgttgtagg cttgacttgc ttcactgtag cggcaaggta
    32701 cgagccaatc gtggagaaaa aaaggagtta atgtaaatta tcatgccctt ttatactgaa
    32761 ccttaagtgc aagtattctt tggtcagtag gaggcattac atcaactgac tttcctcacc
    32821 ctccgcagag cgaaagctaa tactcactaa cgtgccgccg ccggaaggtt gtgaaaaagt
    32881 cagcgtccca cccagccgtt ccgcgcgttc gcgcatgata ttcagaccat aatgaccttc
    32941 gggttctttc ggttcaccga taccaatccc gttatcacgg atataaaccg tgtgattgcc
    33001 gtccggcgcg gtgacgcaac tgacggcgat ttcgctggcg ttggcgtgct tcatcgcatt
    33061 cagcaccgct tcgcgaataa tttgcaacaa atgcacctgc atttgcgcat ccagcgccag
    33121 ggttggcaga cggcagtcga gggtcagttt ggcgctggtt tgattttgta acgtatccag
    33181 catttccctc aacgcggagg ggagatccgc ctgctgcagc gtcaggcgga aagtagtcaa
    33241 cagctcgcgt aactgccgat aagcatcatt caacgcctgg gaaaaatcgg ccatgatact
    33301 ttgtgcggtg gcgttatctt ccggtatcga acgcttaagt aacgtcaact ggatacgtaa
    33361 gtaagaaagt acctgagcca gcgagtcgtg caattcgcgg gcgatggttg cacgttcttc
    33421 catcaacaat aactgctgaa aatgcttctg cgcctgatta aagtacaaac cgcgtcccag
    33481 catcgacgaa acgctgttaa gcagcggttc tgatgatgaa acgtgactat tttgccagtg
    33541 cagttcgccg taaaccgtct cttgcattgt caccggtaaa atctgcatcg gcaattccgg
    33601 gtttggttgc ccttcgctaa tccgccagtt ttcaccgaca tttaactcca gatattcagc
    33661 cgcttcattg tcgcgaacaa tctgcaaaat atggcggaaa caatgcacat caatctggct
    33721 ggtgttcagc gcctgcgaac attgatacaa cacctccaga cgacgcttgg cctcgtggag
    33781 atcgcgggtc ttttcttcta ctgacgcttc cagcgaacgg tacaatttat gcagctcgct
    33841 cgacatctgg ttaaaggttt ttgcaagtag accaagctca ttcggcagac tggtatccag
    33901 cggcggcgag tcgaactgcc cgtgttcaat acgctgactg gcggtaacca gctgattcag
    33961 cggggcaacc acctgatggc gtatgcggcg caaagtaaaa aagaccagcg tgaaaatacc
    34021 gatgccgcca gccagggaaa tcgccaccac cagcagcatt ttgcgttcag cgtagtgctg
    34081 taaagccagc acgaacaggt ctatctgatt aacataatta ttaatattgg cctgatacca
    34141 cggcaaatcg cccttgctga gccgattatt catctccagc cagttggcat tcagatgcgc
    34201 atagcgagtt tttactgctt ctggcacata ccagacgttg aggttggtta ataccggtga
    34261 atgcagtgcc tgctgaaata actggcgatg tgcattgagt tgtggactgc cactttgcaa
    34321 gtcgtagccc aggcggtaac tctgcatacg cagcgatccg gcaatattga tagcctcagc
    34381 gtcgcgcaaa ctgctcgcca gagttagcag agcgataccc gtggaaagaa tcgacagcag
    34441 cacaatgtaa aaaaaggccc gggccagact ggccgagacg ggtcgtttaa caatcacacg
    34501 cgtcttctcc acaaaaaatc attaatgttc cagttcgctg aaacgcaatt tagtcagtga
    34561 tgggattatt tgatctgcca cagtttatag cgaaatagtt acttattctg ggcacatgaa
    34621 cattgccgat tcccctgtaa gtcgggtaat aacaacactc atataaagaa taaggttttt
    34681 acaaccaaaa aagaaggtcg ttatgaatcg ttttattatg gccaacagtc agcaatgtct
    34741 gggttgtcat gcttgtgaaa tcgcctgtgt catggctcac aatgatgagc aacatgtcct
    34801 gagccaacac cattttcatc cccgaattac ggttatcaaa catcagcagc aacgtggtgc
    34861 agtgacctgt caccattgtg aagatgcgcc ctgcgcccgt agctgcccta atggcgcaat
    34921 cagccacgtt gatgacagca ttcaggtcaa tcagcaaaag tgtattggct gtaaatcctg
    34981 cgtggtggcc tgtccgtttg gtacgatgca aatcgtcctg acacccgtcg cagcgggcaa
    35041 agttaaagcc acggcgcata aatgcgacct ttgtgcaggg cgcgaaaacg gtcctgcctg
    35101 tgtcgagaat tgcccggcgg acgcgctgca actggtcact gacgccgcac tctccggcat
    35161 ggcgaaatcc cgccgcttgc gcaccgcgcg tcaggaacat caaccgtggc atgccagtac
    35221 cgctgcacaa gaaatgccgg taatgagtaa agtcgaacaa atgcaggcaa cgcccgcgcg
    35281 tggcgagccg gataaactgg cgattgaagc gcgcaaaacc ggttttgatg aaatttatct
    35341 gccattccgt gccgatcagg cacaacggga agcctcgcgc tgccttaaat gcggcgagca
    35401 cagcgtttgt gaatggacct gcccgctgca taaccatata ccgcagtgga ttgaactggt
    35461 gaaagccgga aacatcgacg ccgccgtcga gctttctcac ctgaccaaca ccctgccgga
    35521 aattaccgga cgcgtttgtc cgcaagaccg tttgtgtgaa ggtgcctgta ctattcgcga
    35581 tgagcacggc gcggtaacta tcggcaacat tgaacgctac atttcagatc aggcgttggc
    35641 gaaaggttgg cgtcctgact taagccatgt caccaaagtg gacaagcggg tggcgattat
    35701 cggtgcaggt ccggcagggc tggcctgtgc ggatgttctg acccgcaatg gcgtggcggt
    35761 gacggtgtac gatcgccatc cagaaattgg tggcttgctc actttcggca ttccttcttt
    35821 caaactggat aaatccctgc tggcacgccg tcgggaaatc ttcagcgcga tggggattca
    35881 cttcgaactc aattgtgaag tgggtaaaga tgtctctttg gattcgcttt tggaacaata
    35941 tgacgcggtc ttcgttggcg taggcactta ccgttccatg aaagcgggtt tacccaatga
    36001 agatgcgcca ggcgtttatg acgcgctgcc gttcctcatc gccaacacta aacaggtgat
    36061 ggggctcgaa gagctaccgg aagagccgtt tatcaatacc gccggactta acgtcgtggt
    36121 actgggcggc ggcgacaccg cgatggactg tgtgcgtact gcgctgcgcc acggcgcgag
    36181 taacgtcacc tgcgcttatc gtcgtgatga agctaacatg ccaggctcga agaaagaggt
    36241 gaagaacgcc cgcgaagagg gggccaactt cgaatttaac gtccagccgg tggcgcttga
    36301 gctgaatgaa caaggtcacg tctgcgggat tcgtttcctg cgcacgcgtc ttggcgagcc
    36361 ggatgcccag gggcgtcggc gtccagtacc ggtggaaggc agtgaatttg tcatgccagc
    36421 cgacgcggtg attatggcgt ttggcttcaa tccgcacggg atgccgtggc tggagtcgca
    36481 cggtgtaacg gtagacaaat ggggccgcat catcgcggat gtggaaagcc agtaccgtta
    36541 ccagaccacc aatccgaaaa tcttcgccgg tggcgacgcc gtgcgtggtg cggatctggt
    36601 ggttaccgca atggcagaag gacgtcatgc ggcacagggg attattgact ggctgggggt
    36661 aaaatcagtc aaatctcact gatagcctgc gcagacaaac ccgacttcac agcgtaatat
    36721 aattgttcat ttcgcgctgt ggagtcggta tgacgcaaca aatcaccctc attaaagaca
    36781 aaattctctc cgataactat ttcaccctgc acaacattac ttacgatctc acgcgcaaag
    36841 atggcgaagt tatccgccat aaacgtgaag tttacgatcg cggtaatggt gcgacgatcc
    36901 tcctgtacaa cgcgaagaaa aagaccgtgg ttctgattcg tcagttccgt gtcgctacct
    36961 gggttaatgg caatgaaagc gggcagctga ttgaaacctg cgccgggctg ctggataacg
    37021 acgaaccgga agtgtgcatt cgcaaagaag cgattgaaga gacgggctat gaagtgggcg
    37081 aagtgcgcaa attatttgaa ctgtatatgt cgccaggcgg tgtgactgag ctaatccact
    37141 tttttatcgc cgaatacagt gacaatcagc gcgctaacgc cggtggcggt gtcgaagatg
    37201 aagatattga agtgctcgag ctgccgttca gccaggcgct ggagatgatc aaaaccggcg
    37261 agatacgtga cggtaagacg gtgttattgc ttaactattt gcaaacgtca catttaatgg
    37321 actgaaaaat aacaataata tttcgttgtt tattgttggc tcaatccgac aaatccgatt
    37381 gagccgcgct acctgcgcaa cgaagatacg acttgtgctg tttgtttgaa cttctggggt
    37441 cgtaccgtcc atgcgctatc gcattttcct tctctttttt ttcgctttgt tgccgacgtc
    37501 tttggtgtgg gcggcaccag cgcaacgggc gttttccgac tggcaggtca cctgtaataa
    37561 ccaaaacttc tgcgtggcgc gtaatacggg cgatcataat ggactggtga tgaccctgag
    37621 ccgcagcgcc ggggcgcata ccgatgccgt tttacgtatt gagcgcggcg ggttgaagtc
    37681 gccggatgcg tctgaagggg aaattgcgcc acggctgctg ttagatggtg agccgttggc
    37741 gttaagtggt gacaagtggc ggatttcacc atggttatta gtaaccgatg atacggcaac
    37801 catcaccgag tttttgcaga tgattcagga agggaaggcg atcactttac gcgacggcaa
    37861 tcagaccatt tctctgagtg gcttaaaagc ggcgttgttg tttattgatg ctcagcaaaa
    37921 gcgtgttggc agtgaaaccg cgtggatcaa gaaaggggac gaaccgccgc tcagcgtacc
    37981 gcccgcgcct gcgctgaaag aggtcgtggt ggttaaccca acgccgacgc cactctcact
    38041 cgaagaacgc aacgatttgc tggattatgg aaactggcgg atgaatggtc tgcgctgctc
    38101 gcttgatcca ttgcgtcgtg aggtgaatgt cactgcgctg actgatgata aagcgctgat
    38161 gatgattagc tgtgaggcag gggcctataa caccattgat ttggcatgga ttgtgtcgcg
    38221 taaaaagcca ctagcctcgc gtccggttcg gttgcgtttg ccgttcaaca acgggcagga
    38281 gacgaatgaa ctggaactga tgaacgcaac atttgatgag aaatcgcgtg aactggtgac
    38341 cttagcgaaa gggcgcggat taagcgattg tggcattcag gcgcgctggc gctttgacgg
    38401 tcagcgtttt cgcctggtgc gttacgccgc agaacccacc tgcgataact ggcatgggcc
    38461 agacgcctgg cccacgttgt ggatcacccg ttaaacaaaa tggcgggaca tgtaggccgg
    38521 ataagacgct gtaggccgga taagacgcgt cagcgtcgca tccggcaatc agcatccggc
    38581 gatcaccatc aggcaccttt tactcccagc accttatgcg cttttgccac aatattctcg
    38641 gcggtaaagc caaagaacgg gaacagctta tccgccggag cagattcccc ataacccgtc
    38701 atcccgacaa ttgccccttt cagaccaaca tacttgtacc agtaatcggc aatacctgct
    38761 tccaccgcca cgcgagccgc aacgttagaa ggcaacaccg actcccgata ttcctcatcc
    38821 tgggcgtcga aaatatcggt cgagggcagg gaaactacgc gaacattgcg accctctccc
    38881 gctaatttct ctgccgcttg cagggtgatt tccatctctg aaccggtggc aatcagaata
    38941 atatccggct tgccgccgct gtctttcaac acataaccac cacgagcaat ctctttaacc
    39001 tgatccggcg tacgttctac ctgggccaga ttctgcctgg agaggatcag tgccgtcggt
    39061 ccgttgtggc gctcaaccgc cagcttccag cccaccgccg cttccacctg atcgcacggt
    39121 cgccaggtgc tgaaatttgg cgttaagcgc aggctggcca gttgctcaac agcctggtgc
    39181 gtcggaccat cttcgcccag accaattgag tcgtgggtat aaaccataat ctgccgcgct
    39241 ttcatcagtg ccgccatccg cgcggcgtta cgggcgtatt caacaaacat caggaacgtt
    39301 gcggtatacg gcacaaagcc gccgtggtgc gcaatgccgt tggcgatagc ggtcatgcca
    39361 aattcacgca ccccgtagtg aatgtagttg cccgccggat cttccttcag cgaaacagaa
    39421 cctttccaga tggtcaggtt gctgggagcc agatccgccg aaccgccgag cagctccggt
    39481 agcatcggcc cgtaagcgtt aagcgtattt tgcgaagcct tacgggtagc gattttcgcc
    39541 ggatttgcct gtaactcatt gatatatttc tgagtcgttt tctcccagtc cttcggtaaa
    39601 ccaccgctca tccgacgggt aaactcttct gccagttgcg gatgagcctt tttataggcg
    39661 gcaaacttct cattccagct ctgctgcgct ttttcgcctt tttcgcgggc atcccaggcg
    39721 tgataaatat ctttagggat ctcaaatggc ggatggtgcc agcccagttt ttgccgtgcc
    39781 agcgccactt cttcttcccc cagcggtgcg ccgtgcgcct cttccttacc tgctttattc
    39841 ggcgaaccaa agccaatcac cgtacggcag ataatcagcg acggcttatc tttcacgctt
    39901 tgcgcttcaa ggatcgcttc cttcaccgcc tgcggatcgt gaccgtcgat ttcatggatc
    39961 acatgccagt gataggcttc aaaacgtttt gccgtatcgt cggtaaacca gccttcggtt
    40021 tctccatcaa tcgaaatacc gttgtgatcg taaaaaccaa tcagcttgcc cagtcccagc
    40081 gtgcccgcca gcgaacagac ttcgtgggaa ataccttcca tcaggcagcc gtcgcccata
    40141 aacacatagg tgaagtgatc gacgatctca tggtctggct ggttaaactg tgcagccagc
    40201 gtacgttccg ctatcgccag cccgacggcg ttcgccaaac cttgtccaag aggaccggtg
    40261 gtggtttcaa ctccgggcgt atagccgatt tccgggtggc caggggtttt cgaatgcagt
    40321 tgacgaaagt tcttcagctc ttccagcggc aggtcgtaac cggtcagatg cagcaaactg
    40381 tagagcagca tcgacgcgtg accgttggaa agaataaagc ggtcgcgatc ataccaggtt
    40441 gggtcggtag ggttatgttt aagaaaatcg ttccacagca cttcggcaat atcagccatg
    40501 cccatcggcg cgccgggatg accagaattg gctttttgta ccgcatccat actgagtgcg
    40561 cgaatcgcat tggcaaggtc ttttcgggac atataacact ccgtggctgg tttatagttt
    40621 ggcggcaaga agatcttcca gtttgcgttg atcaacggcg aacagacgaa tgccttcaga
    40681 cagtttttct accgccatcg catcctgatt gtgctcccaa cggaactccg cttcgctcat
    40741 gggagctggg cgtgggaacg tctgagaagg tgggattaat ttacgtacca ctggcgaaac
    40801 tttttcctgc agctccttca gcaaattcgg tgcgatagtc agtcgatcgc agccggttaa
    40861 ggcgaggatt tgttcggtgc gacggaagct cgcgcccatc acaatggttt catagtggtg
    40921 ttgcttatag tagtcgtaga tattgcgcac cgatttaacg cccggatctt cttccaccac
    40981 atacgggtcc atcggcttgc gtgcctgata ccagtcataa atacgcccga caaacggcga
    41041 aatcagaaaa acgcctgctt ctgcacaggc ccgtgcctgg gcaaaagaaa acagcagcgt
    41101 caggttgcag ttaatacctt ctttttccag ctcttctgcc gcgcgaattc cttcccaggt
    41161 cgaagccagt ttgatcagaa tgcgtgattt ctcaacacct tgttgctgat acaagtccac
    41221 cagatggcgc gccttctcaa tactcttttc tttatcaaaa gagaggcgtg catcaacttc
    41281 tgttgacacg cgaccgggta cgattttgag aatttcagca ccgaaattga ccgccagttt
    41341 gtcacacgct gcgaccacct gttgttcctg ggtcttgcca ttttttttac cccaggcgat
    41401 agcatcgtct attaaatgct catattgtga taatccggca gccttgagta acagcgaagg
    41461 attggtggtg gcatcctggg gatgataatg gcgaatggac tcaatatcgc cgctgtctgc
    41521 cacgacagtg gtgaactgtt tgatgccgtc taactcgttc ataggaaata ctccttgaaa
    41581 agtaaagtgt tagatgagtg cgttaattca cacttctgag aaatttcgct aaacgcatca
    41641 aaaaagcata gcagacaggc atggtattgc tggattaagc aggtaacatc agtgttatag
    41701 gattattacc aaaacattat attaatggtg agagtttgga cttgctcaaa gtctgtagac
    41761 tccggcaagg taataatgtg cgccacgttg tgggcagggg ctgttgccca cacactttat
    41821 ttgtgaacgt tacgtgaaag gaacaaccaa atggatgacc agttaaaaca aagtgcactt
    41881 gatttccatg aatttccagt tccagggaaa atccaggttt ctccaaccaa gcctctggca
    41941 acgcagcgcg atctggcgct ggcctactca ccaggtgttg ccgcaccttg tcttgaaatc
    42001 gaaaaagacc cgttaaaagc ctacaaatat accgcgcgcg gtaacctggt ggcggtgatc
    42061 tctaacggta cggcggtgct ggggttaggc aacattggcg cgctggcagg caaaccggtg
    42121 atggaaggca agggcgttct gtttaagaaa ttcgccggga ttgatgtatt tgatattgaa
    42181 gttgatgaac tcgacccgga caaatttatt gaagtcgtcg ctgcgctcga accgaccttc
    42241 ggcggaatca acctcgaaga cattaaagcg ccagaatgtt tctatattga acagaaactg
    42301 cgcgagcgga tgaatattcc ggtattccac gacgatcagc acggcacggc aatcatcagc
    42361 actgccgcca tcctcaacgg cttgcgcgtg gtggagaaaa acatctccga cgtgcggatg
    42421 gtggtttccg gcgcgggtgc cgcagcaatc gcctgtatga acctgctggt agcgctgggg
    42481 ctgcaaaaac ataacatcgt ggtttgcgat tcaaaaggcg ttatctatca gggccgtgag
    42541 ccaaacatgg cggaaaccaa agccgcatat gcggtagtgg atgacggcaa acgtaccctc
    42601 gatgatgtga ttgaaggcgc agatattttc ctcggctgtt ccggcccgaa agtgctgacc
    42661 caggaaatgg tgaagaaaat ggctcgagcg ccaatgatcc tggcgctggc gaacccggaa
    42721 ccggaaattc tgccgccgct ggcgaaagaa gtgcgtccgg atgccatcat ctgtaccggc
    42781 cgttccgact acccgaacca ggtgaacaac gtcctgtgct tcccgttcat cttccgtggc
    42841 gcgctggacg ttggcgcaac cgccatcaac gaagagatga aactggcggc ggtacgtgct
    42901 attgcagaac tggcccatgc ggaacagagt gaagtggtgg cttcagcgta tggcgatcag
    42961 gatctgagct ttggtccgga atacatcatt ccaaaaccgt ttgatccgcg cttgatcgtt
    43021 aagatcgctc ctgcggtcgc gaaagcggcg atggagtcgg gcgtggcgac gcgtccgatt
    43081 gctgatttcg acgtttacat cgacaagctg actgagttcg tttacaaaac caacctgttt
    43141 atgaagccga ttttctccca ggctcgcaaa gcgccgaagc gcgtagttct gccggaaggg
    43201 gaagaggcgc gcgttctgca tgccactcag gaactggtaa cgctgggact ggcgaaaccg
    43261 atccttatcg gtcgtccgaa cgtgatcgaa atgcgcattc agaaactggg cttgcagatc
    43321 aaagcgggcg ttgattttga gatcgtcaat aacgaatccg atccgcgctt taaagagtac
    43381 tggaccgaat acttccagat catgaagcgt cgcggcgtca ctcaggaaca ggcgcagcgg
    43441 gcgctgatca gtaacccgac agtgatcggc gcgatcatgg ttcagcgtgg cgaagccgat
    43501 gcaatgattt gcggtacggt gggtgattat catgaacatt ttagcgtggt gaaaaatgtc
    43561 tttggttatc gcgatggcgt tcacaccgca ggtgccatga acgcgctgct gctgccgagt
    43621 ggtaacacct ttattgccga tacctacgtc aatgacgaac cagacgcaga agagctggcg
    43681 gagatcacct tgatggcggc agaaactgtc cgtcgtttcg gtattgaacc gcgcgttgct
    43741 ctgttgtcgc actccaactt tggttcttct gactgcccgt cgtcgagcaa aatgcgtcag
    43801 gcgctggaac tggtcaggga acgtgcgcca gaactgatga ttgatggtga aatgcacggc
    43861 gatgcagcgc tggtggaagc gattcgcaac gaccgtatgc cggacagccc tttgaaaggt
    43921 tctgccaata ttctggtgat gccgaacatg gaagctgccc gcattagtta caacttactg
    43981 cgcgtttcca gctcggaagg tgtgactgtc ggtccggtgc tgatgggcgt ggcgaaaccg
    44041 gttcacgtgt taacgccgat cgcatcggtg cgtcgtatcg tcaacatggt ggcgctggcc
    44101 gtggttgaag cgcaaaccca accgctgtaa ttctttttta actctcacgc ttaccctgaa
    44161 tattcagggt aagcggtttg gctgcaatat attagtaaag cttattac
//
LOCUS       DAZUCE010000012        44132 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZUCE010000012 DAZUCE010000000
VERSION     DAZUCE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.012"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(23..2212)
                     /gene="pdeA"
                     /locus_tag="ACHY03_000611"
     CDS             complement(23..2212)
                     /gene="pdeA"
                     /locus_tag="ACHY03_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFS3903888.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2420..2495)
                     /locus_tag="ACHY03_000612"
     tRNA            complement(2420..2495)
                     /locus_tag="ACHY03_000612"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2460..2462),aa:Ala,seq:ggc)
     gene            complement(2535..2610)
                     /locus_tag="ACHY03_000613"
     tRNA            complement(2535..2610)
                     /locus_tag="ACHY03_000613"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2575..2577),aa:Ala,seq:ggc)
     gene            2831..3190
                     /gene="yfeC"
                     /locus_tag="ACHY03_000614"
     CDS             2831..3190
                     /gene="yfeC"
                     /locus_tag="ACHY03_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFS3903889.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3192..3584
                     /gene="yfeD"
                     /locus_tag="ACHY03_000615"
     CDS             3192..3584
                     /gene="yfeD"
                     /locus_tag="ACHY03_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFS3903890.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3624..4832)
                     /locus_tag="ACHY03_000616"
     CDS             complement(3624..4832)
                     /locus_tag="ACHY03_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFS3903891.1"
                     /translation="MKRLQASKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKHIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLEWAYKNFFQKRAAFPR
                     FKKREQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSSGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            4840..5268
                     /gene="tnpA"
                     /locus_tag="ACHY03_000617"
                     /pseudo
     CDS             4840..5268
                     /gene="tnpA"
                     /locus_tag="ACHY03_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5383..6798)
                     /gene="gltX"
                     /locus_tag="ACHY03_000618"
     CDS             complement(5383..6798)
                     /gene="gltX"
                     /locus_tag="ACHY03_000618"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFS3903892.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYFQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            7057..7132
                     /locus_tag="ACHY03_000619"
     tRNA            7057..7132
                     /locus_tag="ACHY03_000619"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7090..7092,aa:Val,seq:tac)
     gene            7177..7252
                     /locus_tag="ACHY03_000620"
     tRNA            7177..7252
                     /locus_tag="ACHY03_000620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7210..7212,aa:Val,seq:tac)
     gene            7257..7332
                     /locus_tag="ACHY03_000621"
     tRNA            7257..7332
                     /locus_tag="ACHY03_000621"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7290..7292,aa:Lys,seq:ttt)
     gene            7452..7793
                     /locus_tag="ACHY03_000622"
     CDS             7452..7793
                     /locus_tag="ACHY03_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFS3903893.1"
                     /translation="MATISSTSIPSIQTQSSNMASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(7784..8710)
                     /gene="yfeR"
                     /locus_tag="ACHY03_000623"
     CDS             complement(7784..8710)
                     /gene="yfeR"
                     /locus_tag="ACHY03_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3903894.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            8800..9798
                     /gene="yfeH"
                     /locus_tag="ACHY03_000624"
     CDS             8800..9798
                     /gene="yfeH"
                     /locus_tag="ACHY03_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFS3903895.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            complement(9795..10013)
                     /gene="ypeB"
                     /locus_tag="ACHY03_000625"
     CDS             complement(9795..10013)
                     /gene="ypeB"
                     /locus_tag="ACHY03_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFS3903896.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(10015..12030)
                     /gene="ligA"
                     /locus_tag="ACHY03_000626"
     CDS             complement(10015..12030)
                     /gene="ligA"
                     /locus_tag="ACHY03_000626"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFS3903897.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(12101..13099)
                     /gene="zipA"
                     /locus_tag="ACHY03_000627"
     CDS             complement(12101..13099)
                     /gene="zipA"
                     /locus_tag="ACHY03_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFS3903898.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            13329..14090
                     /gene="cysZ"
                     /locus_tag="ACHY03_000628"
     CDS             13329..14090
                     /gene="cysZ"
                     /locus_tag="ACHY03_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFS3903899.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            14275..15246
                     /gene="cysK"
                     /locus_tag="ACHY03_000629"
     CDS             14275..15246
                     /gene="cysK"
                     /locus_tag="ACHY03_000629"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFS3903900.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            15630..15887
                     /gene="ptsH"
                     /locus_tag="ACHY03_000630"
     CDS             15630..15887
                     /gene="ptsH"
                     /locus_tag="ACHY03_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFS3903901.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            15932..17659
                     /gene="ptsI"
                     /locus_tag="ACHY03_000631"
     CDS             15932..17659
                     /gene="ptsI"
                     /locus_tag="ACHY03_000631"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFS3903902.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            17700..18209
                     /gene="crr"
                     /locus_tag="ACHY03_000632"
     CDS             17700..18209
                     /gene="crr"
                     /locus_tag="ACHY03_000632"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFS3903903.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(18252..19103)
                     /gene="pdxK"
                     /locus_tag="ACHY03_000633"
                     /gene_synonym="thiJ"
     CDS             complement(18252..19103)
                     /gene="pdxK"
                     /locus_tag="ACHY03_000633"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFS3903904.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVAVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            19208..19576
                     /gene="yfeK"
                     /locus_tag="ACHY03_000634"
     CDS             19208..19576
                     /gene="yfeK"
                     /locus_tag="ACHY03_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFS3903905.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(19579..20490)
                     /gene="cysM"
                     /locus_tag="ACHY03_000635"
     CDS             complement(19579..20490)
                     /gene="cysM"
                     /locus_tag="ACHY03_000635"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFS3903906.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVAMQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(20624..21721)
                     /gene="cysA"
                     /locus_tag="ACHY03_000636"
     CDS             complement(20624..21721)
                     /gene="cysA"
                     /locus_tag="ACHY03_000636"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFS3903907.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNELLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(21711..22586)
                     /gene="cysW"
                     /locus_tag="ACHY03_000637"
     CDS             complement(21711..22586)
                     /gene="cysW"
                     /locus_tag="ACHY03_000637"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFS3903908.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGTGMLVSAFILLVPMVYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFTVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(22586..23419)
                     /gene="cysT"
                     /locus_tag="ACHY03_000638"
     CDS             complement(22586..23419)
                     /gene="cysT"
                     /locus_tag="ACHY03_000638"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFS3903909.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(23419..24435)
                     /gene="cysP"
                     /locus_tag="ACHY03_000639"
     CDS             complement(23419..24435)
                     /gene="cysP"
                     /locus_tag="ACHY03_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFS3903910.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(24593..25384)
                     /gene="ucpA"
                     /locus_tag="ACHY03_000640"
     CDS             complement(24593..25384)
                     /gene="ucpA"
                     /locus_tag="ACHY03_000640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFS3903911.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(25513..26370)
                     /gene="murR"
                     /locus_tag="ACHY03_000641"
     CDS             complement(25513..26370)
                     /gene="murR"
                     /locus_tag="ACHY03_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFS3903912.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            26534..27430
                     /gene="murQ"
                     /locus_tag="ACHY03_000642"
     CDS             26534..27430
                     /gene="murQ"
                     /locus_tag="ACHY03_000642"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFS3903913.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     ATEAKKRLDQHGGFIRQVLDKE"
     gene            27434..28858
                     /gene="murP"
                     /locus_tag="ACHY03_000643"
     CDS             27434..28858
                     /gene="murP"
                     /locus_tag="ACHY03_000643"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFS3903914.1"
                     /translation="MVKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLFATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            28863..30167
                     /gene="pbp4b"
                     /locus_tag="ACHY03_000644"
     CDS             28863..30167
                     /gene="pbp4b"
                     /locus_tag="ACHY03_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFS3903915.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYIPGFADSPSDTIKGKNTLRIADLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVKLFSAETVKIF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(30225..31124)
                     /gene="yfeX"
                     /locus_tag="ACHY03_000645"
     CDS             complement(30225..31124)
                     /gene="yfeX"
                     /locus_tag="ACHY03_000645"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFS3903916.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(31220..31795)
                     /gene="yfeY"
                     /locus_tag="ACHY03_000646"
     CDS             complement(31220..31795)
                     /gene="yfeY"
                     /locus_tag="ACHY03_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFS3903917.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(31856..32305)
                     /gene="yfeZ"
                     /locus_tag="ACHY03_000647"
     CDS             complement(31856..32305)
                     /gene="yfeZ"
                     /locus_tag="ACHY03_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFS3903918.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGFGLALVMADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(32292..32717)
                     /gene="ypeA"
                     /locus_tag="ACHY03_000648"
     CDS             complement(32292..32717)
                     /gene="ypeA"
                     /locus_tag="ACHY03_000648"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFS3903919.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            32931..33800
                     /gene="amiA"
                     /locus_tag="ACHY03_000649"
     CDS             32931..33800
                     /gene="amiA"
                     /locus_tag="ACHY03_000649"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFS3903920.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            33804..34703
                     /gene="hemF"
                     /locus_tag="ACHY03_000650"
     CDS             33804..34703
                     /gene="hemF"
                     /locus_tag="ACHY03_000650"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFS3903921.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(34687..35760)
                     /gene="eutR"
                     /locus_tag="ACHY03_000651"
     CDS             complement(34687..35760)
                     /gene="eutR"
                     /locus_tag="ACHY03_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFS3903922.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGEGVTPNP"
     gene            complement(35806..36306)
                     /gene="eutK"
                     /locus_tag="ACHY03_000652"
     CDS             complement(35806..36306)
                     /gene="eutK"
                     /locus_tag="ACHY03_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFS3903923.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(36319..36978)
                     /gene="eutL"
                     /locus_tag="ACHY03_000653"
     CDS             complement(36319..36978)
                     /gene="eutL"
                     /locus_tag="ACHY03_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFS3903924.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(36988..37875)
                     /gene="eutC"
                     /locus_tag="ACHY03_000654"
     CDS             complement(36988..37875)
                     /gene="eutC"
                     /locus_tag="ACHY03_000654"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFS3903925.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCTTTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(37896..39257)
                     /gene="eutB"
                     /locus_tag="ACHY03_000655"
     CDS             complement(37896..39257)
                     /gene="eutB"
                     /locus_tag="ACHY03_000655"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFS3903926.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(39269..40672)
                     /gene="eutA"
                     /locus_tag="ACHY03_000656"
     CDS             complement(39269..40672)
                     /gene="eutA"
                     /locus_tag="ACHY03_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFS3903927.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(40669..41895)
                     /gene="eutH"
                     /locus_tag="ACHY03_000657"
     CDS             complement(40669..41895)
                     /gene="eutH"
                     /locus_tag="ACHY03_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFS3903928.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAYVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(42012..43199)
                     /gene="eutG"
                     /locus_tag="ACHY03_000658"
     CDS             complement(42012..43199)
                     /gene="eutG"
                     /locus_tag="ACHY03_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFS3903929.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVCLYA
                     AAQ"
     gene            complement(43189..44025)
                     /gene="eutJ"
                     /locus_tag="ACHY03_000659"
     CDS             complement(43189..44025)
                     /gene="eutJ"
                     /locus_tag="ACHY03_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFS3903930.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(44036..>44132)
                     /locus_tag="ACHY03_000660"
     CDS             complement(44036..>44132)
                     /locus_tag="ACHY03_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFS3903931.1"
                     /translation="TITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
BASE COUNT        10493 a        12152 c        11153 g        10334 t
ORIGIN      
        1 ttccccggct tttttacgcc tgttaatcag ctaatggctg cgggcgacca atcaaatacc
       61 cttgcagata ttgcaccccg agcttatgca atagcgcctg ttgctgttgc gtctcgacaa
      121 actccgcgac cacactcaat gacttcgctt tcgccagatc ggtaattgat ctcacaatca
      181 tcgcatccag cgtgttcgtg acgatatctt tcacaaagac gccatcaatt ttgatgatat
      241 cagcctgcaa acgctttaac cgttcgtagt tggcatagcc ggtgccaaag tcatcaatcg
      301 caatccggaa accaaactta tgcagctgct cgatgttgta catgctgctt tctgcgttag
      361 aaaacgcctg ctcctcggtg atctcaagaa tgaccgcctg cggggagatg tgataacgct
      421 taaatagacg aataatccgc ccggcaatat tcttttgcag cagcgtgagc ggcattaaat
      481 taactgaaaa gcgcgggcct tttttgtcgc aagggtgtgt tgccagccac ttcaacaagg
      541 attccagcac ctgcaaatca aaacgcgcac taaggttaaa ctgagcaata aggggcagaa
      601 acttatccgg ggtcataatg ccgccgtcat atttcagtcg cgcgaggatc tcatcataac
      661 cttcaccctc tttgttgcga attggctggg cgtagagcag caaatcaccc tgatctaacg
      721 ccgttcgaat ggtattcagc aatagcacct gtttagtggt ctgcccggaa accatctcct
      781 ctctgctatc cagcgccagc acatgatgat gtgcgcagga ttgctccgcc agccagctta
      841 actgccccaa caagggttgc aaggtttcct gatttccatc aaaacgcccc caggcagcac
      901 catagcccat atccagcccg gtattgttcc agtgaatttg ccgactattc aggatgttaa
      961 ccatatgctg gagtcgccct tccgtttccg gcccgctcag cactaacagc agttcactac
     1021 ccggcaattg atacaacttt tcgttttcct gcatcaatgg cagcagcgta cggtaaattg
     1081 atcggataca gtgaacgcgc atcatcaacc cgtaatgacg actcataaac tcaagattat
     1141 caatgcgcag gcagcaaaaa ctcttgcccg cctcttgctc cggcgcttgt tccaacgcac
     1201 gaaagttggg caggagcgtt aacggatcgg tcagcgcctg caaatgccag cggcgattaa
     1261 gccattcact gcgatgataa atgcgcacca tatagagcag gcaaacgcta aaggaaatca
     1321 gcaccgcgag aataaatgcc agcgaatatt cggtttccac cccttgcaaa aagttctggt
     1381 tgtaattcag caggcaaagc gttgaaacag cccaggtgag atttaaaaac ggatagcgaa
     1441 gcttaccgac cccgagggta aagatgatga agaaaacggg taccaggtaa ccggcaataa
     1501 agtcgttttc ataaggtgtg cacaacagaa gtagcagcac gcttaacgct gccagccagc
     1561 ttaaggtaaa tgcgcgtttc tctttgccca acgacggagc gatatccctg cgccacaata
     1621 tctgcgcaaa gtggggactt acaatcatgc gagtgagata gtagaaaagc atgttgtaaa
     1681 tcagcacagc ggtgaaaagg cttagcaaat caacgaccgt gaaaatggca tccgcatcgc
     1741 cgaaaaaggt ggatatcttt agcgggaaat caaagaagct ccccacaaga tacatgctgc
     1801 atttgatgcc gatcggcgtc accaaaccaa gccagaacag acgttgccag acatatcggc
     1861 tggtcaatcc ataacgccaa cgcgtcccca gctgccagcg taatattgcg caggcacaca
     1921 acacgacaaa cgtctggcta ccgagcatga ccgcagtctg taaaaaagag agatgaaagt
     1981 tccacagatt ggtgcaaaac atcccgcata aaacgggaac gacaccgcgc cagccaaaga
     2041 taaacaagat tgacagcatt acgcacagtg gcatccacgc cagaaagata taactggaat
     2101 ggataatggc aagtggcgaa ataaaacggg aaagctgaat aagtaccacg gtgagcgtaa
     2161 acgctagtgt gaatatcttg atatttttta tcaggttatg ctccacaaac atgagtacac
     2221 tactctcact gatcggggtt ttcaggcaca caaataataa gtttattcgc atcgcgaagc
     2281 aaacaagggc cagcctgttg ccgatttttc atccaatgat gctttcacca ggcggcgcta
     2341 acccggacgt agactgtatt acaaaagcgg caaaaagcag agacaaaaaa cccccgcttt
     2401 gcagcgaggg ttggaaattt ggtggagcta agcgggatcg aaccgctgac ctcttgcatg
     2461 ccatgcaagc gctctcccag ctgagctata gccccacgat gcgtttacgt accaaatttg
     2521 ctgggtgcaa aatttggtgg agctaagcgg gatcgaaccg ctgacctctt gcatgccatg
     2581 caagcgctct cccagctgag ctatagcccc gtcacgtaaa gcttgtcgag ttgacgggcg
     2641 gcatcatatg aattccgccc gaatgtgtca acggcaaatt gcaacgtgta gtttcaatcg
     2701 ctgaaaaatc aggcaaatga acaattttgg aaaaccgctc gcattcagta gttattcatg
     2761 tcacggtttc ctgtaaagtg gtgctataaa atgaactact aatagaccca catacattca
     2821 gggaattgtt atgttcaagg agaggatgac accagatgaa cttgccagac tgaccggcta
     2881 tagccgccag accattaata aatgggtacg caaggaaggc tggacaacgt caccaaaacc
     2941 tggcgtccag ggtggcaaag cccgactggt tcacgtcaat gaacaggttc gtgaatatat
     3001 tcgtaatgct gaacgtccag aaggtcaggg agaagcgcct gccctttctg gtgatgcgcc
     3061 gcttgaagta ttgctggtga cactggcgaa agagatgacg ccagttgaac aaaaacagtt
     3121 tacatccttg cttctgcggg aagggattat cggattgtta caacgcttag ggattcgcga
     3181 tagcaaataa tatgaaaaga ttacgcaata aaatgaccac cgaagaactg gctgaatgcc
     3241 tcggtgtggc taaacaaact gttaaccgtt ggatcagaga aaaaggctgg aaaacggaaa
     3301 aatttcccgg cgtgaaaggc ggtcgtgcca gacttattct ggtcgatacg caagtttgcg
     3361 agtttattca gaacacgcca gccttccata acacaccgat gcttctggaa gccgaagaac
     3421 cccttgccga atacgcaccg gggattcgca ctcctgctta tcggcaaatc atcagcgcaa
     3481 tagacaacat gactcacagt gagcaggaaa aagttgcgca atttttgtca cgcgaaggaa
     3541 ttcgtaattt ccttgctcgt ctcgacatag atgaatcagc ttgacatcct ccacgccctg
     3601 attgacgagg atccctgcta cgttcaggct gtcgcctgaa tcatttcggt gggttcctgc
     3661 ttcaacgggc ggcctgactg caccatccct ccacaggcaa gaacggcgtg ccccgccgct
     3721 aaaatgttac gagcgccgtt tacatcggcg ttcgctgtat atccacatac ctggcatctg
     3781 aatttacttt gtgacaggcg attttctttc gctgtatgac cacagcacgc gcaacgctgg
     3841 cttgtgtacg ctggcggaac agcaagcacc tgaccgccac gccagagctg cttgtacgca
     3901 agctggcggc gcatttcata ccagccctga tccagtatcg aacggtttaa acctgatttt
     3961 gcccggacat tgcgccccgg ctggctgacc gtacccgctg ctgactttga catgttgctg
     4021 accttcaaat cctcaatgac tatcattgcg tggtttttgc tgacggtcgt tgtgactttg
     4081 tgaaggtagt ccctgcggat atttgctata caggaatgca gtcgctgtat tttgcgtttc
     4141 tgcttctgcc agttgttgct gaatttgacc ttgcggctta actgtcgctg aagtctcgcc
     4201 agcttcttct ggtttttctg aaaactgttt acaggctcaa agactgtgcc atctgacagc
     4261 gtggcgagtt tagccacgcc agcatccagc ccgatcattg atgctgaagg gtgaaccgga
     4321 gttgatactt cactttctgt ctgaatactg atgtaccact taccgctgga ctggctgacc
     4381 gtgacatttt tcacaactcc cgtgacctga cggctattcc ggtagcgcat ccagcccagt
     4441 ttcggcagaa aaatacggct gttttcctga tcgagcttaa cgccctgcgg gtagcggaat
     4501 gcatcattct gttcccgctt tttgaatcgg ggaaaagcag cccgcttctg gaagaagttt
     4561 ttgtaagccc actcaaggtc tttcagtgac tgttgcaatg gctgtgaggg cgaatcttta
     4621 agccattgcg tttcagtggc gtttttccac tcaaccagcc aggaagccat tttcccgtaa
     4681 gggatgtgtt tattaccggc ctcatgattc tcattctgac gtgccagagc acgattgaaa
     4741 acgaaacgac atgcacctgc gaagcgcctc atttcacgct cctgttgacc accgggtctt
     4801 aactggaatt tagatgcctg tagtcgtttc acgggtcaaa ttatattttg gtctgtgaaa
     4861 aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcgcctggtc
     4921 tttgtcacca gataccgacg tcagattttt gaccatgacg cgacagaaaa acgacgcact
     4981 tacttttcaa atgtatgtgc tgattttgaa gctaaactgg ttgaaatgga tggcgaacca
     5041 gatcacgtcc atttgttaat taactatcct cccaaactgg cgatattcag tctggtaaac
     5101 agcctcaaag gcatatcgaa taggttactg cgacgagatc gaccagatat tgcagtcagg
     5161 tattactaca aaggcgttat gtggagtcct ggctattttg ccagtagctg cggaggtgcg
     5221 ccaatatccg tcatccgcca atacattgaa caacagcaaa cacctggtca ggtggaaaac
     5281 cgcgccttat atccccgccc tgaaggacgg ggttttacgg cgcaccggat aaataaaaaa
     5341 cggcaggggt atttctcctg ccgtttatct tttatgcgct aattactgct gattttcgcg
     5401 ttccgcaata aaagccagcg ctttgttgat acgctcgata ctgcgggtct taccgatcgc
     5461 gtgaacggta acgtccagcg ccggagactg ccccgcaccg gttacggcga cacgcagcgg
     5521 cataccaact ttacccatac ccacttccag ctcatccgcc gtcgcctgaa tagcgtgatg
     5581 aacgttttca gcggtccagt cagtaatcgc ggccagtttg tcacgaacca cttccagcgg
     5641 ctgacgcgct accggacgca gatgtttttt cgcggcgtcg gcatcgaact cagcaaaatc
     5701 ttcgtagaaa taacggcagc tctgtgccat ctctttcagc gtcttgcagc gttcgcccag
     5761 cagtttcacc agatcagcca gctgcgggcc gttacgggta tcgatatttt cctgctcaat
     5821 gtgccactgt aagtgagtag caacatactc cggcggcagc gcgttaatgt agtgatggtt
     5881 cagccacagc agcttgtcgg tgttgaacgc actggcagat ttgctgacgg cattcaaagt
     5941 gaagtatttg atcatctctt cacgagtgaa gatttcctga tcgccgtggg accagcccag
     6001 acgcaccaga tagttcagca gtgcttctgg caaataaccg tcatcacgat actgcattac
     6061 gctgaccgcc ccgtgacgtt tggacagttt tttaccgtca tcgccgttga tcatagaaac
     6121 gtgcgcgtaa accggcaccg gcgctttcag ggccttaaga atgttgatct ggcgtggcgt
     6181 gttgttgata tggtcttcgc cacggataac gtgggtgatt tccatatccc agtcatcgac
     6241 aaccacacag aagttatagg ttggggaacc atcggtacgg cggataataa gatcgtccag
     6301 ttcctggttg ctgaactcga tcggaccacg gatctgatcg tcaaaaacaa cagaaccttc
     6361 ctgcgggtta gcaaaacgta cgacacacgg ctcatcatca gcgtgatgct catgactgtg
     6421 gcggcagcga ccgtcataac gcggcttctc acctttcgcc atttgctctt cgcgcagcgc
     6481 ttccaggcgc tctttagagc aatagcattt ataagctgtg ccctcttcca gcatctgatc
     6541 gatcaccgcg ttgtagcgat caaaacgttt ggtctggaag tacggacctt catcccactc
     6601 caggctcagc cagttcatgc catccataat ggcttcgata gcttccggcg tggaacgctc
     6661 aagatcggtg tcttcaatac gcagcacgaa ctcaccgccg tgattacgtg caaaaagcca
     6721 ggagtaaaga gcagtacgcg cgccgccaac gtgcagatag cctgttgggc ttggcgcgaa
     6781 gcgagttttg attttcatga aatggcctta cgtttagaaa gatgccgaca accggcaaat
     6841 cctggaaaaa ttgagtgggc gatattctat cactcccgcc tgattcctca atgtagttcg
     6901 ggttgcaacc ttacgcatta ttgttttcgt tgacaaattg cgcaacacgg tctgttttgc
     6961 gatcatcttg cttaaattta cgacgaacga acaatttctt tagaaaatgc gttgactcat
     7021 tttgaactct ccctataatg cgactccaca cagcgggggt gattagctca gctgggagag
     7081 cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac caactactat
     7141 atgtagtctc cgccgtgtag caagaaattg agaagtgggt gattagctca gctgggagag
     7201 cacctccctt acaaggaggg ggtcggcggt tcgatcccgt catcacccac cacttcgggt
     7261 cgttagctca gttggtagag cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg
     7321 cacgacccac caatgtaaaa aagcgcccta aaggcgcttt tttgctatct gcgatttgta
     7381 agattgattt ttcttctcaa gttccttgtt cttctaccga tattacctcg tacagtgacg
     7441 aggactgaac aatggccact atctcttcaa cttcaattcc ttccatacaa acacagagta
     7501 gtaacatggc atcacaaggt agcgatgttg cttcacaaat cgcgcggata agtcagcaaa
     7561 tcatcaaact cactcaacaa attaaagaga ttgtagacac tagcggcagt gctgaagaca
     7621 aacaaaaaca ggccgaattg attcaacagc aaatcaccct actggaaaca caactggcgc
     7681 agttgcagag acagcaggca gaaaaggcgc aggaaaaaga gcagcgtttg tctctgaatg
     7741 tatcccttct aaatcctgtt gaaaaaacga ctcatatcga tatctatatc tgatacaacg
     7801 gatccccttc ccgcccagcc attagcgcat tgccctggtc tcgtaccacg tcccataacg
     7861 cttcagcggc ggtggaaagt gaacggttct tcctgcgcac cagcatcaac tggcgttcaa
     7921 caaccggcgt aatgcgcttt accaccagcg ggctaccttc aggcaatggt agcgccagtg
     7981 cgggaagaat actgatgccg atgcctgcgg ctaccatcgg gaatagcgtc gccggatgtc
     8041 caatctcctg cacaatattc gcctgaatac cattgcgcgc cagcgctgcg tcaatcagcg
     8101 gtcggctgcc tgacgcgtaa tcctgcaaca ccaatttcgc tccctgtaat gcctgccagg
     8161 gtacataatc ttccgcggcc aaagcgctat cccgatggca aagcagaaag aaaggctcgg
     8221 aaagaatcgc ttcacattgc agatcgccca ctgggcctgg atcgatgacg atgccaaagt
     8281 cgacatctcc ctggcgaata ctttccatca cccactgctg cggacgatcg tgcaggacaa
     8341 actgaatatc tggatagcgg cgatggcttt cagcaatgca ttgcggaata agatgcgcgg
     8401 aaatggtctg gctggcagcg acccgaactt ttccgctcag ttgttgcccc atacgcccgg
     8461 tatcgcgcaa cgtgctgtta agttcatcca gcagtcgctc aagacgcaaa gccaactgct
     8521 gccctgcatc tgtaagcacc acttcacgcg tggttctgtc cagcaggcga acaccagtat
     8581 gattttccag ctccttcaca ctgtgactta ctgccgactg gctcaggccg atacgctctc
     8641 ctgcacgact aaaacttttc tcctgcgcta ctgtgacgaa aacctttaat tgttttaaag
     8701 aataattcat ctgttttctt catgaatgga tgcaataaat caattttatt tctcaaactg
     8761 gaagaagcac aatagaacca tcgatcatct ggagtcttta tgaaactttt tcgtatcctc
     8821 gatcctttca ccttaaccct gatcacggtg gtgttgctgg cctctttctt tccggccaga
     8881 ggcgatttcg tccccttctt tgaaaatctg accaccgcag ctattgccct gctgttcttt
     8941 atgcacggcg cgaagttgtc gcgtgaggcg attattgctg gcggtggtca ctggcgactg
     9001 catttgtggg taatgtgcag caccttcgtg ctgtttccga ttctgggtgt actgtttgcc
     9061 tggtggaaac cggtaaatgt cgacccgatg ctctactccg gttttctcta cttgtgcatt
     9121 ctcccggcta ccgtgcagtc tgcaatcgcc ttcacatcaa tggcgggcgg taacgtcgcg
     9181 gcggcggttt gttctgcgtc agcatccagc ctgctgggga ttttcctttc accattgctg
     9241 gttggtctgg tgatgaatgt tcacggtgca gggggcagcc ttgagcaggt cggtaaaatt
     9301 atgctgcaac tgctgctgcc gtttgtgttg gggcatcttt cccggccgtg gattggtgac
     9361 tgggtgtcgc gcaataaaaa atggattgcg aaaactgacc agacgtccat tctgttggtg
     9421 gtttatacag cgttcagcga agccgtcgtt aatggtatct ggcataaagt tggctgggga
     9481 tcattgctgt ttatcgtggt ggtcagctgc gttcttctgg ctatcgtgat tgtagttaac
     9541 gtctttatgg cacgccgact gggcttcaat aaggcagatg aaattactat cgtcttttgt
     9601 ggttcgaaaa agagtctggc aaatggcatc ccgatggcaa acattctgtt ccccacatcg
     9661 gtgatcggta tgatggtgct gcccctgatg attttccatc agatccaatt gatggtctgt
     9721 gcggtgctgg cgcgtcgata caaacgccag accgaacagt tacaggcgca gcaggaaagc
     9781 agcaccgata aagcttaaag cggacgcttc aggggctgga ccagttgcgt cagcccctcg
     9841 gttttaatca gcagcgtgat ttgcattagc tcaccgagct tccctgccgg gaactcatct
     9901 ttgcgggcaa accacagcag atactcctcc ggcaggtcga ttaagcgacg ccctttgtat
     9961 ttgccaaacg gcattatcgt attggctatt tcaatcagct gctctttttc catctcagct
    10021 acccagcaaa cgcagcattt ccgcttcgtc gatgacttca atgcccagtt cctgcgcctt
    10081 cgccagttta gatcctgcag cttcacccgc tatcaccaga tcggttttct tcgacacgct
    10141 gcccgcgact ttcgcgccca gttcgaccag tcgagcttta gcgtcatcac gcgacatctg
    10201 gcttaagctg cccgtaagca ccacggtttt accagcaaac gggctgtcaa tctcttccgc
    10261 gttgataacg atcggcgcag gccagtgaac accttccgcc aacagctcgc tgatgacatt
    10321 gcggttgctt tcttcggcaa agaagttgtg aacgtgggat gcaacgacaa tgccaacatc
    10381 aggcaccttt tgcagctctt caatcgaagc ggcttccagc gcttccagcg tgccgaaata
    10441 tgccgccaga cctgctgcgg tggcctcgcc gacttcacgg atgccaagtg catagaggaa
    10501 gcgagcaaag gtggtttctt tcgctttttc cagcgcgtta accacgtttt gtgccgattt
    10561 tggccccata cgctccagtc cggtaagttt gcctgcggtg agtttgaaca gatccgccgg
    10621 agtgtggaca tattcttttt caaccagctg atcgatgatt ttgtcgccca ttccgtcaac
    10681 atccatcgca cggcgggaaa caaagtgttt cagcgactct ttacgctgcg caccgcaaat
    10741 caggccaccg gtacagcggg caaccgcttc accttccaca cgctcaacgt cagaaccaca
    10801 taccggacaa tgcgtcggga atacaacctc acgagtatct tccgggcgtt cagaaagcac
    10861 gacgttcacc acctgcggga tcacgtcgcc agcgcggcga atcaccactt tatcgccaat
    10921 gcgtaaacca agacgttcga tttcatccgc attgtgtaag gttgcgttac tcaccagcac
    10981 gcctgcaaca tggacaggtt ccagacgcgc aacaggcgta atcgcgccag tacgcccaac
    11041 ctgaaactcg acgtcacgca caaaggtcat ctgttcctgc gccgggaatt taaacgctac
    11101 cgcccagcgc ggggctcgcg cgacaaagcc aagctgctcc tgctgtgcca gtgagttgac
    11161 cttaatcacc acgccgtcga tatcaaagcc cagcgtcggg cggtcttctt ccactttgtg
    11221 atagaacgcc agcacttctt ccgccgattc acaaagcgtt acccgatcgc tgaccggcaa
    11281 cccccacttt ttaaattgca gtaaacggcc aagatgagta tccggcagct cgccaccttc
    11341 cagaacacca acgccatagc agaaaaaagt gagcggtcgc ttcgctgtaa tacgcggatc
    11401 aagctgacgc agtgaaccag ctgccgcatt acgtgggtta gcaaaaactt tcccgcccgt
    11461 gcgtcgcgca tcttcgttaa tcttttcgaa ccccgcctgc ggcaggaaca cttcaccacg
    11521 cacttccaga cgcgccggga tattctctcc gtgcagcttc agtggaatgg cgcgaatggt
    11581 acgcacgtta gacgtgatat cttccccggt ggtgccatcg ccacgggtcg cggcactgac
    11641 taaaacgcca ttttcataca gaatactgac agcaaggcca tccagcttca gctcacagca
    11701 ccaggtgact ttctcgttgc ttttcagacg gtcctgcact cgtttgttga aggcaagaaa
    11761 gctttcttca tcaaaaacgt tatccagcga cagcattggc acttcatggc gaatctggct
    11821 gaaagccgcc agcggcgcag cacctacacg ttgggtaggc gaatcaggcg taatcagttc
    11881 tggatgtttg gtttccagct cgcgcagttc gcgcatcagc ctgtcgtatt cagcgtcggg
    11941 aatttccggc gcatccatca catgataaag atattcatga tggcgaagcg tcgttcgcag
    12001 ttctgtcagt tgttgttcga ttgattccat atcgcaccat caatgctaaa aacccccgac
    12061 aagcgggggt tcgaagagga gttaatttgc cttaagtgta tcaggcgttg gcgtctttga
    12121 cttcgcggat gatgtcctgg tactcacgca atttctgcgg agtcatcata cggcgctgat
    12181 cgtcaagcac gacaccgccc acttcatcgg caatatgctg cgcagattgc agcatcagct
    12241 tgaagttctg cagctcgtca ccgtaagacg gtacctgcat aaagatagtg acacccggag
    12301 tagtgaaatc cttcatttca ggatcaaagg ttcccggttt caccatattc gccaggctga
    12361 ataacgccgg gccgctgcca tccgggctaa gatgacgatg gtaaatattc atatcgccaa
    12421 aaatgaagcc cgcttgttga atgctgttaa gaagcagttc accgtttagc tcgctaccgt
    12481 gatgcgccgc gacgttcatg ataatcaccg cttctttgcg cttcggttta tccataactg
    12541 gagccggttc cgctacaggc tcaggctgtg gtgccgctac gggttctgca ggctggaaag
    12601 cctgttgtgc cggttgcggt gctgaatgca ctggctgcgg cgctggcgcg acctgtggcg
    12661 aaactggctg ctgcaacggc tgttcaggct gcggctgata ggcaggctgc tgcacgggtt
    12721 gctgcaccgg ctgcgctgga cgcggagcat gttgcggcgg tacctgcgct tcaggcggct
    12781 gctggaccgg ttgacgcggc tgcgcagacg cataaggcgg ttggtactgg tgttgcggcg
    12841 acggacgagc agcctcatgc tcctgggcgt tagccggggc atgattcacg cggtgaacac
    12901 gaacctcacc aacgccctca tcatcttcga catcctcgtc ataagaatcg tcgtcacgtt
    12961 ttgacttcat tcgttttaat ggccgatcgc ggaacataga agatcgttct ttacggctgg
    13021 tccagaaacc atgtaccagt aaagcgatta tggcgatcgc gccaacaatg attaatatca
    13081 gacgcaaatc ctgcatcatt atattctctg ttgttctaac accttgccac cacggcaaac
    13141 atttactcac taagagtatt tgccgattac ctcaagtgca agtgcactat taactttcac
    13201 agcacaaaga tagatgaaat cgtgcttttt gctgtttttt cgaacatatc ctaactgtcc
    13261 attgcgcaat tacccggtaa aatacgcaca attttcctgg gattggtcaa aaggagctca
    13321 tcctgattat ggtttcatca ttcacatctg ccccacgcag cggtttttac tattttgcgc
    13381 aaggctggaa gctcgtctcg caacctggga ttcggcgttt cgttatttta ccgctgctgg
    13441 tcaatatttt gttgatgggg ggcgcattct ggtggctctt tacgcaactc gatgtctgga
    13501 tcccgactct catgagttat gttccggatt ggttacaatg gctgagctat ctgttgtggc
    13561 ctctggctgt catctctgtg ctgttagtgt ttggctattt cttctccacg attgctaact
    13621 ggattgccgc tccgtttaac ggtttattgg ctgaacaact ggaagcacga ttgactggcg
    13681 ctacaccgcc agataccggg attttcggta tcatgaaaga tgtgccgcga atcatgaaac
    13741 gcgaatggca aaaatttgcc tggtatctgc cgcgcgcaat tgtattacta attctttact
    13801 tcatccctgg tattgggcaa accgtcgcgc cggtactgtg gttcctgttt agcgcctgga
    13861 tgttagccat ccagtattgc gattacccct tcgataacca caaagtgccg tttaaagaga
    13921 tgcgcaccgc cctgcgcaca cgcaaaatca ccaatatgca gtttggtgct ttaaccagcc
    13981 tgtttacgat gatcccgctg cttaatctgt tcattatgcc cgttgccgtt tgtggcgcga
    14041 cggcgatgtg ggtcgattgc tatcgcgata aacacgcgat gtggcggtaa caatctaccg
    14101 gttattttgt aaaccgtttg tgtgaaacag gggtggctta tgccgcccct tattccatct
    14161 ttcatgtcat tatttccctt ctgtatatag atatgctaaa tccttacttc cgcatattct
    14221 ctgagcgggt atgctacctg ttgtatccca atttcataca gttaaggaca ggccatgagt
    14281 aagatttttg aagataactc gctgactatc ggtcacacgc cgctggttcg cctgaatcgc
    14341 atcggtaacg gacgcattct ggcgaaggtg gaatctcgta accccagctt cagcgttaag
    14401 tgccgtatcg gtgccaacat gatttgggat gccgaaaagc gcggcgtgct gaaaccaggc
    14461 gttgaactgg ttgaaccaac cagcggtaat accgggattg cactggccta tgtcgctgcc
    14521 gctcgcggtt acaaactcac cctgaccatg ccagaaacca tgagtattga acgccgcaag
    14581 ctgctgaaag cgttaggtgc aaacctggtg ctgaccgaag gtgctaaagg catgaaaggc
    14641 gcaatccaaa aagcagaaga aattgtcgcc agcaatccag agaaatacct gctgctgcaa
    14701 caattcagca atccggcaaa ccctgaaatt cacgaaaaaa ccaccggtcc ggagatctgg
    14761 gaagataccg acggtcaggt tgatgtattt attgctggcg ttgggactgg cggtacgctg
    14821 actggcgtca gccgctacat taaaggcacc aaaggtaaga ccgatcttat ctctgtcgcc
    14881 gttgagccaa ccgattctcc agttatcgcc caggcgctgg caggtgaaga gattaaacct
    14941 ggcccgcata aaattcaggg tattggcgct ggttttatcc cggctaacct cgatctcaag
    15001 ctggtcgata aagtcattgg catcaccaat gaagaagcca tttctaccgc ccgtcgtcta
    15061 atggaagaag aaggtattct tgcaggtatc tcttctggag cagctgttgc tgcggcgttg
    15121 aaactacaag aagatgaaag ctttaccaac aagaatattg tggttattct accgtcatcg
    15181 ggtgagcgtt atttaagcac cgcattgttt gccgatctct tcactgagaa agaattgcaa
    15241 cagtaatgcc agcttgttaa aaatgcgtaa aaaagcacct ttttaggtgc ttttttgtgg
    15301 cctgcttcaa actttcgccc ctcctggcat tgattcagcc tgtcggaact ggtatttaac
    15361 cagactaatt attttgatgc gcgaaattaa tcgttacagg aaaagccaaa gctgaatcga
    15421 ttttatgatt tggttcaatt cttcctttag cggcataatg tttaatgacg tacgaaacgt
    15481 cagcggtcaa cacccgccag caatggactg tattgcgctc ttcgtgcgtc gcgtctgtta
    15541 aaaactggcg ctaacaatac aggctaaagt cgaaccgcca ggctagactt tagttccaca
    15601 acactaaacc tataagttgg ggaaatacaa tgttccagca agaagttacc attaccgctc
    15661 cgaacggtct gcacacccgc cctgctgccc agtttgtaaa agaagctaag ggcttcactt
    15721 ctgaaattac tgtgacttcc aacggcaaaa gcgccagcgc gaaaagcctg tttaaactgc
    15781 agactctggg cctgactcaa ggtaccgttg tgactatctc cgcagaaggc gaagacgagc
    15841 agaaagcggt tgaacatctg gttaaactga tggcggaact cgagtaattt cccgggttct
    15901 tttaaaaatc agtcacaagt aaggtagggt tatgatttca ggcattttag catccccggg
    15961 tatcgctttc ggtaaagctc tgcttctgaa agaagacgaa attgtcattg accggaaaaa
    16021 aatttctgcc gaccaggttg atcaggaagt tgaacgtttt ctgagcggtc gtgccaaggc
    16081 atcagcccag ctggaaacga tcaaaacgaa agctggtgaa acgttcggtg aagaaaaaga
    16141 agccatcttt gaagggcata ttatgctgct cgaagatgag gagctggagc aggaaatcat
    16201 agccctgatt aaagataaac acatgacagc tgacgcagct gctcatgaag ttatcgaagg
    16261 tcaggcttct gccttggaag agctggatga tgaatacctg aaagaacgtg cggctgacgt
    16321 acgtgatatc ggtaagcgcc tgctgcgcaa catcctgggc ctgaagatta tcgacctgag
    16381 cgccattcag gatgaagtca ttctggttgc cgctgacctg acgccgtccg aaaccgcaca
    16441 gctgaacctg aagaaggtgc tgggtttcat caccgacgcg ggtggccgta cttcccacac
    16501 ctctatcatg gcgcgttctc tggaactgcc tgctatcgtg ggtaccggta gcgtcacctc
    16561 tcaggtgaaa aatgacgact atctgattct ggatgccgta aataatcagg tttacgtcaa
    16621 tccaaccaac gaagttattg ataaaatgcg cgctgttcag gagcaagtgg cttctgaaaa
    16681 agcagagctt gctaaactga aagatctgcc agctattacg ctggacggtc accaggtaga
    16741 agtatgcgct aacattggta cggttcgtga cgttgaaggt gcagagcgta acggcgctga
    16801 aggcgttggt ctgtatcgta ctgagttcct gttcatggac cgcgacgcgc tgcccactga
    16861 agaagaacag tttgctgctt acaaagcagt ggctgaagcg tgtggctcgc aggcggttat
    16921 cgttcgtacc atggacatcg gcggcgacaa agagctgcca tacatgaact tcccgaaaga
    16981 agagaacccg ttcctcggct ggcgcgctat ccgtatcgcg atggatcgta aagagatcct
    17041 gcgcgatcag ctccgcgcta tcctgcgtgc ctcggctttc ggtaaattgc gcattatgtt
    17101 cccgatgatc atctctgttg aagaagtgcg tgcactgcgc aaagagatcg aaatttacaa
    17161 acaggaactg cgcgacgaag gtaaagcgtt tgacgagtca attgaaatcg gcgtaatggt
    17221 ggaaacaccg gctgccgcaa caattgcacg tcatttagcc aaagaagttg atttctttag
    17281 tatcggcacc aatgatttaa cgcagtacac tctggcagtt gaccgtggta atgatatgat
    17341 ttcacacctt taccagccaa tgtcaccgtc cgtgctgaac ttgatcaagc aagttattga
    17401 tgcttctcat gctgaaggca aatggactgg catgtgtggt gagcttgctg gcgatgaacg
    17461 tgctacactt ctgttgctgg ggatgggtct ggacgaattc tctatgagcg ccatttctat
    17521 cccgcgcatt aagaagatta tccgtaacac gaacttcgaa gatgcgaagg tgttagcaga
    17581 gcaggctctt gctcaaccga caacggacga gttaatgacg ctggttaaca agttcattga
    17641 agaaaaaaca atctgctaat ccacgagatg cggcccaatt tactgcttag gagaagatca
    17701 tgggtttgtt cgataaactg aaatctctgg tttccgacga caagaaggat accggaacta
    17761 ttgagatcat tgctccgctc tctggcgaga tcgtcaatat cgaagacgtg ccggatgtcg
    17821 tttttgcgga aaaaatcgtt ggtgatggta ttgctatcaa accaacgggt aacaaaatgg
    17881 tcgcgcctgt agacggcacc attggtaaaa tctttgaaac caaccacgca ttctctatcg
    17941 aatctgatag cggcgttgaa ctgttcgtcc acttcggtat cgacaccgtt gaactgaaag
    18001 gcgaaggctt caagcgtatt gctgaagaag gtcagcgcgt gaaagttggc gatactgtca
    18061 ttgaatttga tctgccgctg ctggaagaga aagccaagtc taccctgact ccggttgtta
    18121 tctccaacat ggacgaaatc aaagaactga tcaaactgtc cggtagcgtg actgtgggtg
    18181 aaaccccggt tatccgcatc aagaaataat tcttgccgca gtgaaaaatg gcgcccatcg
    18241 gcgccatttt tttatgcttc cgccagcggc ggcaaaatca attcatcgct ctcatgctgc
    18301 tgggtgtagc gcatcacttc cagtacgcgc aaccccgcgc ggtgcaccgc atcgtttaat
    18361 gccttccctt tcagcaaacc actgataagc tgagcacaaa acaggtcgcc agtccctttc
    18421 aggtcggttt ttacccttga atgggaaatg acattcacgc tgtcggcact gaccaccgcc
    18481 acctgcatct cctgattttc ttcattaccg gaggcgctgg taatcaccac ccattttaat
    18541 gtgtctgaaa gcagactttt tgcggcagca atggcactgt cgagatcgcg gcaattttta
    18601 ccggtcagga tttccagctc aaagatattg ggggtaattc cctgcgccag cggcagtaaa
    18661 tattgtcgat acgcttcggg aaggtcaggt ttgacataaa ttccgctgtc aatatcgcca
    18721 atcaccggat cgaccatgat caataggtca ggatggtctt tgcgtagcgc agtcagccac
    18781 tcggcaagga ttttgatttg cgatgccgtt cccatatagc ccgtggttac agcacgaagt
    18841 tggcgcagcg catcacgctc ctgaagcgca cgcaaatagc cgctaaacca ttcgtccgga
    18901 atcgcaccac cgtagaaagt gtcataatgc ggcgtattgc tcagcaatac cgtcggcacg
    18961 gcaaagacat tcaggccgtt ctgtttgata gcaggcacgg caatgctgtt gcctacgctg
    19021 ccgtacacta cctgcgactg cacggcgacg atatccgcct gcagtgccct gctcttatcg
    19081 ttaaacaaca acaaactact catttaattt tttctccttg ccgatgatcc tcatcgtatt
    19141 ccaaccgaaa ctttacctga ttctggcagt caaatcggct attacaaaac aaggataagg
    19201 taattcagtg aagaaaatca tttgtctggt cattacacta ctaatgacac tccccgctta
    19261 cgcgaagtta actgcccatg aagaagcccg catcaacgcc atgctggagg gattagcaca
    19321 gaaaaaggat ttgatatttg tgcgcaacgg tgatgaacat acctgcgatg aagcggtttc
    19381 tcatctgcgt ctgaagctgg gcaatacccg aaaccgcatt gacactgccg agcagtttat
    19441 tgataaggtt gcttcgtcgt catcgattac cgggaagccg tatattgtga agatgcccgg
    19501 taaaagcgat gagaacgcac aacctttttt acatgcgcta attgcgcaga cggataaaac
    19561 ggtgcctgcg caataatctt aaatccccgc cccctgacta aaatgctctt ccccaaacac
    19621 cccggtggaa aggtagcgat cgccacgatc gcagatgatc gctaccacca ccgcaccagg
    19681 gttagctttt gccacccgca gtgctccggc aaccgcgccg ccggagctga cgccacagaa
    19741 tattccttcc cgcaccgcca gttcgcgcat ggtgttttcc gcatcgcgct gatgaatatc
    19801 cagcacctca tccaccagcg aagcgttgaa aatccccggc agatattccg caggccagcg
    19861 gcgaatgccc ggaatactgc tgccttcttc cggttgcatg gcaacaatgg tcaccggttt
    19921 ggattgttcg cgcataaagc gtgaaacgcc ggtgatagtg ccggttgttc ccatgctgga
    19981 gacaaaatgg gtaatgcgcc cgccagtctg ctgccagatt tccggcccgg tagtggtgta
    20041 atgggcgtaa gggttatcgg gattattgaa ctgatcgagc agctttcctt caccacgatt
    20101 cgccatctcc agcgccagat cgcgcgcacc ttccatgccc tgctctttgg tgacaagaat
    20161 cagttccgca ccataagcac gcatcgccgc acggcgttcc tggctcatgt tgtcgggcat
    20221 cagcaatttc atgcgatagc ctttaagcgc ggcaatcatt gccagcgcaa tgccggtgtt
    20281 accactggtg gcttcgatta acacatcacc cggtttaatt tccccgcgct tttccgcctc
    20341 gacgatcatc gaaagcgccg cacgatcttt caccgaacct gccgggttat tgccttccag
    20401 ttttaaccac acttcactgc cgttatccgg ccccattcgc tgcaacttca ccagaggcgt
    20461 attgcctatt gtttgttcta atgtactcac gatctttatc catacgtggt gtcgcctgat
    20521 gcgacgctta cgcgtcttat caggtctaca ggttacaaac cttgccatta aaaagcccgg
    20581 atcgcgggaa gcgcctccgg gcgtttaaca ttcactcaac ctatcaggcg ctttgtgcga
    20641 gagcaagttc ctcatcgcgg gtttcaatac gctcgtcgcc gttatacagc cgcgcatgtt
    20701 gcagaccaac gaataaacgc tcgccacgct gcggggcatc gtcgccatgc atcacgaccg
    20761 tcagcagttc gttgtaccac cccagcggct gcaccactaa ttgggtgtag tgacctttcg
    20821 ggctggcttc cagtacctgt accggcagcg gcgaatcgag actggtacgg cggctgatat
    20881 ccacttccca cgggcgcagg aagagatcca ccggcccctg atacgcaggt gtgtagccca
    20941 gcggccagcg atgggcgcca acatggaact gcccgccgcg aatggttccc tgcaggcggt
    21001 tcacttcgcc cataaactcg agcacaaaac gggtcgctgg ttcgcgccat acctggtccg
    21061 gcgcatcagc ctgctcgata ttgccctggc tcatcaccac tacacgatca gctacttcgg
    21121 tcgcttcttc ctggtcgtgg gtcacgaaca cgctggtgaa tttcagctct tcatgcagtt
    21181 gacgcagcca gcgacgcagc tctttacgca cctgcgcatc cagcgcgcca aacggttcat
    21241 caagcagcag aatttgcggt tccacagcca gcgcgcgcgc cagcgccacg cgctgtttct
    21301 ggccgccgga aagctgtgcc ggataacgat ccgccagatg agcaagctgc accatctcca
    21361 gcaattttgt cactttcgct ttgatcgccg cggcattcgg gcgctcgcga cgcggcagca
    21421 ccgtcaggcc aaaagcgata ttgtcgaaca ccgtcatatg gcggaacagc gcgtaatgct
    21481 ggaacacgaa accgacttta cgatcacgtg cgtgcaggcg gctcacgtcg gtaccgtgga
    21541 agcgaatatg cccgctggtt tgatgctcca gcccggcgat aatccgcagc agcgtggttt
    21601 ttccggaacc ggacggcccc agcaacgcga ccatctgacc tgaaggaata tccagtgaga
    21661 tatcgttcag cacctgggtg cgaccaaacg acttcttaat attggcaatc tcaatgctca
    21721 tgatgttcct cctgctgtgc gcgtttttcc tgattctcca ggcgccactg caacatactt
    21781 tttaaaaaca gggtgataat cgccatcagc gttaacagcg cggcggcggt aaaggagccg
    21841 actgtgttgt agtcctgctc cagcaattca atctgtaacg gcagcgacag ggtttcgcca
    21901 cgaatcgagc cggaaaccac cgacactgcg ccaaactcgc caatcgcgcg ggcgttggtc
    21961 agcaccacgc cataaagcag cgcccagcgg atgttcggca atgtgacacg gcggaacatc
    22021 tgccagccgg atgcgccaag caaaatcgcc gcttcgtctt cctgactgcc ctggcttaac
    22081 atcaccggca ccagttcgcg caccacaaac ggacacgtca cgaagatagt gaccagcacc
    22141 attccgggcc aggagaacat aatttgcaga ttatgttcat ccagccagcc gccgagcggg
    22201 ccgttggagc cgtagaacag cagatacacc agaccggcaa ccaccggtga cacggtaaaa
    22261 ggaatgtcca gtagcgtcag cagtaactgg cgtccgggga agttaaagcg cgtcaccagc
    22321 caggccagca gaatgccgaa caccaggttt accggtacgg caatcagcgc aatcatcacc
    22381 gtcagccaga tggcgtgcag catgtccgga tcggccagat tctgtaaaac cggcatcagc
    22441 cccttgctga atgcctgcac gaagatgtaa accatcggca ccagcaggat gaacgccgaa
    22501 accagcatcc cggtgccaat cagaaaccat ttgccccagt taatcgggcg cgcgtcataa
    22561 cgcttcaact gtgtcacttc cgccattaat gacctaccac acgccgacca aagcgacttt
    22621 gcagagtgtt aattgagaac agcagcagca gagatgccgc gaggatcacc gaggcaatcg
    22681 cgctcgccgc cgggtaatca aactcctgca agcgcacaaa aatcatcagc gacgtcactt
    22741 ccgtcttcca cgcgatattc ccggcgataa aaatcaccgc gccaaactca ccgaggctac
    22801 gggtaaacga caacgccacg cccgccacca gcgccggaga aagctccggc agcaccactt
    22861 tgcagaaact ctgccagcgc gttgcgccaa gcgtttctgc cgcctcttca tattccgggc
    22921 ctaactcttc cagcaccggc tgcacggtac gtaccacaaa cggaatgctg gtaaaggcca
    22981 tcgccaccgc aatcccaagc caggtgtagg tgactttgat atcaaacttc gccagccatt
    23041 caccgtaaaa accgttcacg gaaaagagcg aggccagcgt taaaccggcg actgccgttg
    23101 gcagcgcaaa gggtaaatcc atcagcgcat caagcagcgt gcggccaggg aagcgataac
    23161 gagttaagat ccacgccatc agcaggccga aaacgccgtt aaaaatcgat gccacaaacg
    23221 ccgacagcag cgtcactttg taggctgcga ccacctgcgg gttggtgatc acctcccagt
    23281 actgcgccca gctcatctgg gccagctgca tcaccagcgc ggagagtggc aggagcaaaa
    23341 tcaggcaaac aaacagcaga ctggtgccga ggcttaaggt aaagcccggc agcacgcgtc
    23401 ttgatgaaac tgcaaacatc agttacgccc cgccgctaac agcttgtcta actcgccgcc
    23461 gctggtgaag tgggttttca tcacttccgg ccaggagcca aatttgtctt ccacacggaa
    23521 cagttcggtc tgcgggaatt tatctttcag tttgtccatg acttccgggt tatttacgcg
    23581 gtagtaatag tcggtgatga tggtctgcgc ctgcgggctg tagagccagt tcaggtaggc
    23641 ttttgcggct ttttccgtac cgttggcctg cacgttttta tcgacccacg ccaccgggaa
    23701 ttccgccaga atgttggttt tcggaatcac cacttcaaag ccctgcgctt cgtactgttt
    23761 gcggatgttg ttcacttccg actcgaagct gatcaataca tcgcccagac cgcgctcggc
    23821 gaaggtggtg gtcgcgccac gaccgccggt atcgaacact tcaacgtttt tcaggaactg
    23881 agtcataaac tgttcggttt tggctttgtc gccaccgtca gctttgtccg ctgcgcccca
    23941 tgccgcaagg taggtgtaac gcgcgttacc agacgttttc gggttcggaa aaatcagctt
    24001 cacgtcggag cgcaccaggt cgttccagtc gtggatattc ttcgggttac ccttacgcac
    24061 caggaagccc atggtggagt agaacggcga gctattattc ggcaggcgcg tttgccagtc
    24121 cgccgggatc agcttgcctt tgtcatgcag gatttgtacg tcggtcacct ggttataagt
    24181 tacaacgtcg gcttttaagc cctgcaaaat cgccagcgcc tgttttgatg acccggcatg
    24241 agattgtttt atcgtcagtt tgtcgccgcc gttatctttg gcccattgtt gctcaaacgg
    24301 cgggttcagg gcggcaaaca gctcgcggga gacgtcataa gaactgttca gcagttccgt
    24361 tgcctgtaca tggcccgcca gcagcagaga agcgaccagc gcgagtgagt tctttttcag
    24421 taagttaacg gccattgcgc acccttataa atttaatgac tttctaatag ccatcatatt
    24481 tataacggac gtgaaaggag taacggtttt atataccgtt tggtgatttg gaagttgaaa
    24541 agggaataag acgaaatcct ccccacacat gcagggagga aacagaggtg aatcagatac
    24601 cgacgctgac cgtctccggc agtgtgctgc cgccatcaat cacattctgt gtaccggtta
    24661 aatagctgga ttcatccgat gcgaggaagg ccgccagttc gccgacttcc agcggatcgg
    24721 cgaggcgacg cagcgggatt gcttttgcca tatcagtcag caccgactct ggatcttccg
    24781 ggttcgactg gcgggcaatg ctttccgcca ttggcgtgcg gacgtatccc gggcagatgg
    24841 cgttaacgcg aataccggat tgcgcgtact ccaccgccag cgatttagtc aggccaacaa
    24901 tcgccgcttt cgttaaggcg tacgccgttt cacccggatc ggccaccata tcgccagtga
    24961 ccgaagacat catcacaatg cgaccatctt tgcgcgcaat catctccggc agcaccgcct
    25021 tcgtgacgtt ccatacgcct ttaatattga tgtcaatatg gaaatcgcga tcgtcatcgc
    25081 tcatgtcgag gaaactgccc agacgacaaa caccggcgtt attcaccagg atatcaatgc
    25141 gcccttcttt ttccttcgcg cgcttgatag ctgcggctac cgatgccggg tcacgcacat
    25201 cggcgacaac cgccgtacag cgatgaccac gaccacacag ttcgtccgcc agcttttcga
    25261 tctcagggga gatatccagc aagattaggt ttgcgccatg acgcgcaaaa gttctggcaa
    25321 ttccttcgcc aattccctgc aatgcgcccg taatcagtgc tgtcttgccc gtgagtttac
    25381 ccattttaat gctctccttg ttaatacaga gcgttacact ccattccctc tgaaaataca
    25441 ggataaacaa aatattgctg cggatcagct cactaattca ggaaaatctc aatccagtcg
    25501 ctgctttatg catcatttca ggcgttgtgt tagctcactg ctgcgctgaa tcattttcaa
    25561 tgactccaca tcattgagtt gcacaagacc aacaaacagt aaatcggtaa cagaattttg
    25621 cgccgtgcgg gtagacatcg acgagctacg ccattcggtt tcaccggaaa cggtatcgag
    25681 cgtgaaatgc gccagccgcc gcagcggaga gtcagccaga gaagtaatgg caatcaccgt
    25741 tgccccctgc tttcgtgccg cttctgcgca caagacaatc tcttttttac tgccgctgta
    25801 agagatagcg atttgtacat cgcctttttt caacgcctgg gaaacggtgg cttgtacgtg
    25861 cgtgtcggct tcgcaggcca cgcgatatcc aattttcatc agtttgaacg acagatcgcg
    25921 ccccaccagc gccgatccgc ccaggcccgt tatctgaata aattgcgctt tactgatgac
    25981 atcaatgatt ttttgcagcc gtgcgtagtc gaacaacgcg caggtttgct ccagcgccag
    26041 ctctttttcg cgattcagtt tgcgggcaat cacttccagc gaatcatcac tggtaatcga
    26101 actgtgcagg tgcagcgccg tggcattcgt tttctcccgg ctggcactgt attcgcctat
    26161 taacgccata cgcaattcag taaatccttg tgcgccgagc ttctgggcaa acttcacaat
    26221 actcgactgg ctgatgccga gctgtttcgc catcttgcgc gaagagacgg atttcagctc
    26281 actcacgttg gcccgcagaa agtcagcaat tttttgctcg ttctcggtaa attcactttc
    26341 cgcattgcgt attttcgtca ggtacaacat cttccactct cctgggctgg cagcaaaaat
    26401 tccgcttaag ttcttatgac gctcttcaca ctctgcgagt gtagactcaa tgattccttt
    26461 aagtctgtca attcgggata tatgattcca tacatctgca attatttgat cgtaaatagt
    26521 aaggtcacca ccgatgcaac ttgaaaagat gattaccgaa ggctcgaacg ccgcctcggc
    26581 tgaaattgac cgcgtatcaa cgctggaaat gtgccggatt atcaacgatg aagataaaac
    26641 cgtaccgctt gccgttgagc gcgtactgcc ggatatcgcc gcggcgatcg atgttatcca
    26701 cgcccaggta agcggcggcg gtcgtctgat ttatctcggt gcgggaacat ccggtcgtct
    26761 ggggattctg gatgccagcg aatgtccgcc cacctacggc gttaaaccgg gtttggtggt
    26821 tggtttgatt gctggaggcg aatatgccat tcagcacgcg gtggaaggcg ctgaagatag
    26881 ccgggaaggt ggtgttaacg atctgaaaaa tattaattta acggcacagg atgtggtggt
    26941 tggtattgcg gccagtggtc gcacgccgta tgtaattgcc ggactggaat acgcacgcca
    27001 gctcggctgc cgcacagtgg gaatttcctg taatccgggg agcgccgttt caaccaccgc
    27061 agagtttgcc attacaccga ttgtaggtgc cgaagtggtt accggttctt cgcggatgaa
    27121 agcaggtaca gcgcagaaac tggtgctcaa tatgctctcc accgggctga tgattaaatc
    27181 cggcaaagtg ttcggcaacc tgatggtcga tgtggtcgcc accaacgaaa aactgcatgt
    27241 gcgccaggtc aatatcgtta aaaacgccac cggatgtaat gccgaacaag cggaagcggc
    27301 gttaattgcc tgcgagcgca actgtaaaac ggccattgtg atggtgctga aaaatctcga
    27361 tgccacagaa gctaaaaaac gcctggatca acacggcggc tttattcgtc aggttttaga
    27421 caaggaataa cccatggtca aagagatcag cagtgaactt ctgaacacca ttcttacccg
    27481 tgtcggcgga ccgggaaata tcgccagttg tggtaactgt atgacgcgcc tgcgtctggg
    27541 tgtacatgac agttcactgg ttgatcccaa tatcaaaacg ctggaaggcg tgaagggcgt
    27601 cattttgacc agcgaccagg tgcaggtcgt ttttgggcct ggtaaagcgc atcgtgccgc
    27661 aaaagcgatg agcgagctgc tgggagaagc accggtacag gatgccgcag aaatcgccgc
    27721 ccagaacaaa cgtcagttaa aagccaaaca aacctccggc gtgcaacaat ttctcgctaa
    27781 attcgccacc atctttacgc cgcttatccc cggttttatt gccgccggtc tgctgctggg
    27841 gatagcgacg ttatttgcca cggtgatgca cgttccggca gatgctcagg gcacgctacc
    27901 cgacgcgctg aattttatga aggtgttcag caaaggtttg ttcaccttcc tggtgattct
    27961 ggtgggctat aacgccgccc aggcatttgg cggcacgggc gtcaatggcg caattattgc
    28021 cgcactcttt ttgctcggtt ataaccccgc tgcaaccacc ggttactacg ccggttttca
    28081 cgatttcttt ggtctgccca tcgatccgcg cggcaatatt atcggcgtgt tgattgccgc
    28141 gtgggcctgc gcccgcatcg aaggcatggt gcgccgcttt atgcccgacg atctcgacat
    28201 gctgctgacc tcgttaatca ccctactgat caccgctacg ctggcctacc tgatcattat
    28261 gccgctgggc ggctggttat tcgaaggtat gtcgtggctg tttatgcacc tgaacagtaa
    28321 tccgctcggt tgtgcggttt tagccgggct gttcctgatc gccgtggtat ttggcgtgca
    28381 tcagggcttt attcctgttt acctcgcgtt aatggacagc cagggattca acagcttatt
    28441 tcccatcctt tcgatggcag gcgcgggcca ggtgggcgcg gcgctggcac tctactggcg
    28501 ggcgcaaccg cacagtgcat tacgcagtca ggtacgcggg gcgattattc ccggcctgct
    28561 gggcgttggc gaaccgttga tttatggcgt caccctgccc cgcatgaaac cgtttattac
    28621 cgcctgttta ggcggcgcgg cgggcggttt gtttatcgga ttgatagcct ggtggggtct
    28681 gccgatgggc ttaaacagcg cctttgggcc gtctgggctg gtagctctgc cgttgatgac
    28741 ttcagcacaa ggcattcttc cggcgatggc agtctacgct ggcgggattc tggtggcatg
    28801 ggtttgcggg tttattttca ccacgctttt tggctgccgt aacgtcaatc tggactgata
    28861 tcatgaaacg gacaatgctc tatctttctc tgctggctgt tagttgtagc gtcagcgccg
    28921 caaaataccc tgttctgaca gaaagttcgc cagagaaagc agggttcaac gtcgaacggc
    28981 ttaaccagat ggatcgctgg attagccagc aagtggacgc cggttatccc ggcgtaaacc
    29041 tgctgattat aaaagataat cagattgtgt atcgcaaagc ctggggagcg gcgaaaaagt
    29101 acgatggcag cgtattgatg gaacagcctg tcaaagccac caccgggacg ctgtatgacc
    29161 tggcttccaa taccaagatg tacgccacta actttgctct gcaaaagcta atgagcgaag
    29221 gcaagctgca tcctgacgat ctgattgcga agtatattcc agggtttgcc gatagcccga
    29281 gcgatacgat taaaggcaaa aacacgctgc ggatcgccga cctgctgcat cacagcggcg
    29341 gtttcccggc ggacccacaa tatccgaata aagcggtcgc gggcgcgtta tattcccagg
    29401 ataaaggcca gacgctggag atgatcaagc gcacgccgct agagtatcag cccggcagca
    29461 agcatattta cagtgatgtc gattatatgc tgcttggatt tatcgtcgag tcggtcaccg
    29521 gtcagccgct tgaccgctat gttgaagagt cgatttatcg cccgctcggc ctgacacata
    29581 cggtatttaa cccgctgctg aaaggcttta aaccgcagca aattgccgcg actgaactga
    29641 acggcaatac ccgcgatgga gtgatccact tcccgaatat ccgcacttcc actctgtggg
    29701 gtcaggtaca cgatgaaaaa gccttttatt cgatgggcgg cgtttccggg catgcaggtt
    29761 tgttttccaa taccggcgat attgcggtgt taatgcaaac gatgctgaat ggcggtggct
    29821 atggcgatgt gaagctgttc agtgcggaaa cggtgaaaat attcaccacc agttctaagg
    29881 aagatgccac ttttggcctc ggctggcgcg tgaatggtaa tgccaccatg acgccgacgt
    29941 ttggcacgct ggcaagcccg caaacctacg gtcacactgg ctggaccgga acggtgaccg
    30001 ttatcgatcc ggtgaatcat atggcgattg tgatgttaag caacaagcca cattcgccgg
    30061 ttgccgatcc gcaaaagaat cccaatatgt tcgaaagcgg tcagctgccg attgcaacgt
    30121 atggttgggt agttgatcag gtgtatgcgg cgttaaagca aaagtaatta aaaggccagt
    30181 cagtaatgtt actgcctggc ctttaatcaa tgaatcagaa acgcttacag cgccatcaac
    30241 ttgtccagcg acggtgcgaa ataatagccg ccggttaccg gtttggtgaa acgcaacatc
    30301 gcatcacgct taccatcggt atcgccaaac atgctcagca gttgctgctc aatgttatgc
    30361 agacgcgcgc agtaggcgca gaagtacaga ccgtgcgtac cactggcggt gccgtacggc
    30421 aggctctggc gaacaatctt cagccctttg ccatcttctt tcagatcaac gcgggtgagg
    30481 tgagaggttt ccggacgttc gtcgccgtca atctcttcgt tggcctcttt ggtgcgcccg
    30541 atcatcatct cctgatcgtg aacgctcatc cggttgagct gcttcaggtt gtgctcccaa
    30601 cgctgcacaa acacatagct gccgccggca tccacgccgt ctttgataac cgccacttcg
    30661 cgacgcgtct cttcacccgc cgggttttct gtaccgtcaa caaagccgct caggtcacgc
    30721 tcttccaccc aacggaagcc gtggatctct tctttcactt caatgcagtc accaaaggct
    30781 tccatcgccg cctgggcgac agagaagttt acgtcgtgac gcagagaaag aatgtggatc
    30841 aacacatcga actgggtggt cggcgcaagg cctttaccgt agcccggaaa atctttcagc
    30901 tcttctgccc caacgccgcc gctcagagcg cgccaagtgt tgttaccaaa ggcaaccacc
    30961 gcaccaagat gcgcgtccgg gaatttcgct tcaaaagttg ccagtttgtc ggcaaatgtt
    31021 ttactggccg cacgcagggc gtcaacttcc cctttcacgt tggcttcgat ccaaatcgcc
    31081 gcgcggcaat gttctggcaa aatgccactc tgaacctgag acattgttcc tcctgaaaat
    31141 aataatgcca cgcaatccgt ggcattgttg agcgctattt tacgtgtttt ttcgcggcgg
    31201 cggtttgttc agacgcaaat taacgccgcc agataatttt actgactttc cagtttttca
    31261 gggtatcgtc cgaaggcatt aacccttcag gaccactcca ttccccgctg aactggtagc
    31321 taatatgttg actgccttcg gctttacatt cgacggcacg attatcatca ccatcagctt
    31381 tttggcaatt gccaaatgct ttgctgtaaa ggtcgctaaa cggtgtaccg attttaacac
    31441 cggtgtcagc aggaatatcg ctatccagca cgtcaatgcg actgatcgtg ccttgatcgc
    31501 cgttaatcac catcgccacg ttgtcgccct tcatcacttc aaaaaagcgc accacgttgc
    31561 cgttcgcggt tttcattccg ctgcgcaggc gataatcgcc atcaagcgca tcggcaatgg
    31621 cttgttcttg cagtggtgtg gacgccgtta attcaccaac gccctgctcg ctcactttgg
    31681 tggacgaccc aaaccagttc cacgggttag cggcagacca gttaactgac gacatcgtgg
    31741 aacagccggt cagcatcaat ggcatagcgc ataacattaa acgcagcgat ttcatgtcac
    31801 ttcctttggt tattcaataa cgttgcttgg agtgcaaatt caccaaaaag tgccgttatt
    31861 cttttacttc attaaaacaa gcgcgtaaac gtcgattggt cagcagccag attaacgcca
    31921 caatatccgc cattaccagc gccagaccga aaccgctaac ggattcaccg ttcagccaca
    31981 gccacggttg ccagcaaagt aaaaccactt gcgccaacag caacagaaaa tagagcacat
    32041 gccagatacg gggaaacgaa gcccgccgac cactcagcaa aaacgccagc accgccggaa
    32101 tgccaggaat cagccccagc cagaaattat cgtgatcggg ataaaacaga ttcagtagcg
    32161 cagtaccctg ctcgcgcgac gcaccggcaa taacaaacag cacccaggtt cgcgcctgaa
    32221 gcaatagcac aagccagaag agcaagggta aacgcaggcg accgtgcgca tcataatgga
    32281 caggatgaaa ctcagtactc ttcatcttca atcaaacgct tacccagact cagcacgtcg
    32341 gcgtgttcat atcccaggcg ttcatacatt ccgagcacca tgtcgttatc ttccggcacg
    32401 ttgatctgaa ttttcgggca gccgcgagca atcagctttt tctccagccg gttaagcaac
    32461 gcattggcaa tcccacgccc acgaaactct ggatgcacgc caagataata agcagacccg
    32521 cgatgcccgt cataaccgcc catcaccgtt ccgaccactt caccgtttac ctcagcgacc
    32581 agaaacaaac tgacgtcatg gttcatctta cgctcgatgt ccatttccgg atcgttccac
    32641 ggacgcagca agtcgcaacg ctcccaaagg gtgatgacct cttcgaaatc ttcctggcga
    32701 aatacgcgta tctccatggt attcgttacc tttttgcggg ttaaaaggct gattatggcg
    32761 tgaacggtcg aattagccaa tatctgacga aaatcggttg aaaaagtggc ataatgggga
    32821 gttgtcaact attgaaatga aaagtaaaac aattctcaac agcaaacggt cgtaacggat
    32881 tacgcgatac gatataacat ctggaacttt attattacaa ctcaggccgt atgagcactt
    32941 ttaaaccact aaaaacactc acttcgcgcc gccaggtgct gaaagccggt ttggctgccc
    33001 tgacgttgtc aggaatgtcg caagccatcg ccaaagacga acctttaaaa accagcaacg
    33061 gacacagcaa gccgaaagct aaaaaatctg gcggcaaacg tgtcgttgtt ctcgatccag
    33121 gccacggcgg gattgatacc ggagcgatcg ggcgcaacgg ttcgaaagaa aaacatgtgg
    33181 tgctggcgat tgctaaaaac gtccgttcca ttttgcgtaa tcatgggatt gatgcgcgtt
    33241 taacgcgttc tggcgatacg tttatcccac tttacgatcg cgttgaaatc gcccataaac
    33301 atggcgcaga tctgtttatg tcaattcatg ccgatggctt taccaacccg aaagctgccg
    33361 gtgcttcggt atttgccctc tctaaccgtg gggcaagtag cgcaatggcg aaatacctgt
    33421 ctgaacgcga aaaccgcgcc gatgaagttg ccggtaaaaa ggcgactgac aaggatcacc
    33481 tattgcagca agtgctgttt gatctggtgc aaaccgatac tattaaaaac agcctgacgc
    33541 ttggctcgca tattctgaag aagattaagc cggtgcataa actgcacagc cgcaacaccg
    33601 aacaagccgc gtttgtggtg ttgaaatcgc cgtcggttcc ttcggtgctg gtggaaacct
    33661 cgtttatcac caacccggaa gaagagcggc tgttaggcac ggcggcgttt cgtcagaaaa
    33721 tcgccacagc gattgctgaa ggcgtgatca gttatttcca ctggttcgac aaccagaaag
    33781 cacattcgaa aaagcgataa gttatgaaac ccgacgcaca ccaggttaaa cagtttctgc
    33841 tcaaccttca ggatacgatt tgtcagcagc tgaccgccgt cgatggcgca gaatttgtcg
    33901 aagatagctg gcagcgcgaa ggtggcggcg gcgggcgtag tcgggtgttg cgtaatggtg
    33961 gtgttttcga acaggcaggc gtcaactttt ctcacgtcca cggcgaggcg atgcctgctt
    34021 ccgccaccgc tcatcgcccg gaacttgccg ggcgcagttt cgaggcgatg ggcgtttctc
    34081 tggtagtgca tccgcataat ccgtatgttc ccaccagcca cgcgaatgtg cgctttttta
    34141 ttgccgaaaa accgggtgca gagccagtct ggtggtttgg cggcggcttc gatttaaccc
    34201 ctttctatgg ttttgaagaa gacgccattc actggcaccg caccgcccgt gacctgtgcc
    34261 tgccatttgg tgaagacgtt tatccccgtt acaaaaagtg gtgcgacgat tacttctacc
    34321 tcaaacatcg caacgaacag cgcggtattg gcgggctgtt ctttgatgat ctaaacacgc
    34381 cagatttcga ccactgtttt gcctttatgc aggcggtagg caaaggctac accgacgctt
    34441 atttaccaat tgtagagcga cgtaaagcga tggcctacgg cgagcgcgag cgcaattttc
    34501 agctctaccg tcgcggtcgt tatgtcgagt tcaatctagt ctgggatcgc ggcactctgt
    34561 ttggcctgca aactggcggg cgcaccgagt cgatcctgat gtcaatgccg ccactggtac
    34621 gctgggagta tgattatcag ccaaaagatg gcagcccaga agcggcgtta agtgagttta
    34681 ttaaggtcag ggattgggtg taactccctc accccattcc cgcatccgct gatgcaacgt
    34741 caacgacggc ttctcggcaa acagctgctg gtaatccgtg gcaaattgcc ccagatgcca
    34801 gaatccccac tgcatggcgg cgtcttttac cgttgtgctt tgcgaccacg ggcttatcag
    34861 ttcgcggcgt acggcgttca ggcgaatgcg tttcagccac gcgttcgggc caatgcctaa
    34921 aatagcgtga aacgcgtttt gtagcgtgcg gcggctgaca tgcagttgat tacacaagtc
    34981 cagcaccgtc accggttcgg acatgttttc cagcacatat tcacgggcgc gggaaagcaa
    35041 ccgacggtaa ctctgatgac tgatgctttc cgccgtcacc attggctgcg cttcttccag
    35101 catcgccccc atcgccatta gcaaattatc ccccagcact tttcgcactg caggctgatg
    35161 aagattttcc gggttctcgc aaaacgtcgc cagcgcctgt tggacaaagc cccacagcgc
    35221 ggctttatgc tgctctttca cttccagcgc cgactggcta cgcaacatat gtaatacccg
    35281 atccggatta tgcaaaaagt tagcctgacg ggtgatgaca tcttcagaaa gcactacgcc
    35341 caggatcgtg taatcgtccg gcgtacttaa ttcaaactcg gttccaccag ggcgcgtcgc
    35401 tatttctgcg cttcccagac attgcgaacc gataaatccc tgctcaccgc gcgtcgccgg
    35461 aatgccaaac cagaacgagt tcggccagac cagacacgac tgacgcagcg ccagaccggt
    35521 gtattcacga aaaacctgaa tatcatcgag tagaatttcc gtaaattcac catgaaactt
    35581 gcccggatgc agctgatcgt aaatctgctg ccaggcggta atcgttaaag catgttcata
    35641 gacatccgtt gtctgtcgtt gatgaacatt atccacttcg accttcggcg tgagcttcag
    35701 gttttcgggt aagggttcat gataaagatg gtgcaaattg gctgtacggg tctttttcat
    35761 gatgttaatg ccgggtgttg taggacaccc ggcacctccg acaggttaat ggggcttgag
    35821 acgataacga ctactgcgtt tacgtaacgt cccggcagaa aagagctgtt cgagcgcatt
    35881 tctggctttt tccagcggcc agccaaagtg ggcagccact tctcctgccg tcattccctg
    35941 acgtactgat gttaacagcg ccaacaactc gtcagcagat tccgcgacga taactggcgc
    36001 gtcgggttcc tttacgggtt gcttcggctg gcggttaaag ccagtaacca gccactgggt
    36061 gtcatcgtct ggccgaccga tctccttacg gctgatgaca cgcccggtac gcatcgcggc
    36121 agcacaacct gcgtccagcg cagcacgaca cgccgccaga tcgccttcca ccaccagcgt
    36181 taagcgacca gggtcaagca cttcgtgact gagcagacga acgttagctg ctttgagcat
    36241 ggcatccgct gcatctatcg cagcgaccat gccgtccact tccagcaatc ccagtgcatt
    36301 gatcatcggc aacctccatt acgcacgctg gattggatta cgcgcgattt ccagcactgc
    36361 atcggtaaag gcgttacagg ccgctttaca cgctgcctgg ctaccggtta aaaatgctgc
    36421 cgagtagtta gtttcagacg gtggcgggac ataggtcacc agctgtacgt cggcagattt
    36481 caacgctgca tcaataccgt aggtcgcttc cagcggcggt gccaccagat acgccatcgg
    36541 atcgccaagc gtgatcccgg cggttgatga gagataagaa ccggtacgcg aaactacatg
    36601 tgccaggaat gccgtatctt gcgcatcatt agcccactgg aaagcagcgc cattttcaat
    36661 atgcgcaacc atcgcatcca gacctgcacg cacttccgcc gggtttggcc caccaagcat
    36721 aatcagcact tcaccggcag tcggtgacgg gccgtgtgca gcgcccgcat acagcgagcg
    36781 gccatacacc acttccacca tcgcctgttt ggtcgcttcg tcagccgcaa tgtaggtgac
    36841 gtcatcggaa tcagcagaaa tcagcccgag gctacgaata tgcggcggca atttcagttc
    36901 acgcgcaaaa tcggcgttaa cggaggcaat cacccgcata gcggtgacag acggtcgaat
    36961 caaatctaaa gctggcatga tgcctcctta tcgggtcatg ttgatgccgg atgctttctg
    37021 ctccagcata cgtttcgcca aatccacaat gacggctgcg gcttcaaccg gcggcgtgcc
    37081 gccctggtga atgttagaga tacaggtgcg atcggcctca acggtggtcg ccatacgcgg
    37141 cgagtaaacg gcgtagcagg agaggctttc tgactgcccc aaccccggac gttcgcccac
    37201 cagcaggatc actactttcg cgccgaggat ctcaccgatc tgatcttcaa tcttcacgcg
    37261 accataacga acaaagaatg gtgtaccgac tttcagaccg gcctgtttca ggcccgccat
    37321 cagcggtggc aggatctctt cgtagttcac ggtgatcgca tcagttgaca ggccatcaga
    37381 aataacgacc tgcacgtccg gattggcaac acactgcgct ttcagcgctt caacagcttc
    37441 tgcacacagg cggcggccca tatccgggcg agtcaggtag agatttttgt cgctaatttc
    37501 agagcgtact tccagcaagc cctgcgcttt cacccactct tccggcactt ctttcagtac
    37561 ggtatctttc gaacgagagt gatcggccag gaaacgcagc agcgcctggg tacgcggacg
    37621 cggaccggca cgaccggtac aaacgcgggc cacggtgctg cgacgcagtt ctgttaacac
    37681 atccgcacga tgtgggtttt caacaccaat ccacgctttt gcttccgcag aacctaaatc
    37741 cagcgcacag ctttccgagg tcaccggtgc cgcacaggtg gtggtggtgc actttgcttc
    37801 tgacggcgcg ggggccgttt gtcccattga cgccatcacg ctgcgtacaa tttcttcaat
    37861 ctgtttttga tccatgatgt gttatctccg cgtcatcaga agaacagtga cggatcgccc
    37921 gcccgtttgg tcaggcgacc gtttgccata atgcccatgc tttccagcca gcgttcaaac
    37981 tccggtgacg ggcgcaggtt gagtaactga cgcacagtgg cggtatcgtg gaatgcggtg
    38041 gtctggtaat tgagcatgat gtcatcaccc agcggcatcc ccatgatgta gttgcagcct
    38101 gcggtggcga gcaggatcat caggttttcg ttgaggttct ggtcagcgtc agcgtggttg
    38161 gtgtaacagc agtcacagcc catagagatg ccgctcagct tgcccataaa gtgatcttct
    38221 aagccagcac ggataatctg gcggtcgttg tagagatact ccggcccaat aaaaccgacc
    38281 acggtgttga cgataaacgg atcgtaatga cgcgccagcc catagttacg tgcttccatc
    38341 gtcacctggt cagcgccgaa gttagcgcca gcggatagcg cagagccttg tccggtttcg
    38401 aagtagaggc agttttcccc ggcgatacgg ttgaactctg cgcccactgc gcgcgcttcg
    38461 tcgagcatcg ccagttccac gccaaactct ttcagccctt tttcgctgcc acagatactc
    38521 tggaaaatca gtccgccagg cgcgccgcga cgaatcgctt cgatctgggt ggtgacgtgc
    38581 gccagtacgc agccctgagt tgggatgttg aatttgtcga tcacgccata aatggtatcc
    38641 agcacgcggc ttaagttttc cacgtcgtca gtcaccgggt taacgccgat caccgcatcg
    38701 cccaccccga aggaaagccc ttcgtagatt tgcgcggcga tactttgcac gtcgtcacgg
    38761 gtgtcatttg gctgcaaacg ggcgctaaag gtgcccggaa taccgatggt ggtattggcc
    38821 tttttgatta ccggcatttt cttcgcgccg tagatcaggt ccgcgttgga gcaaatcttc
    38881 gctaccgccg cgaccacttc cgaggtcagc cctttgcggg taaaggcaat gtcgtccacg
    38941 ctggtttcat cgctcagcac atactcacgc agttcgctga tgctccagtt tttaatctgg
    39001 ttgtaggccg tttcgttaac atcgtcctga atcagccgcg tcacgcagtc atcttcatag
    39061 gcaatcaccg gattattgcg gatgtccgct acggtcattt ccgacaacac ctgctttgcc
    39121 gccacgcgct cctgtgaact tgccgctgcg acgcccgcca gcacatcccc cgaacgcagt
    39181 tcgttggctt tagccagcac ctcttttaca tccttaaact gatatacatt gccgaacaat
    39241 gtggtcttta gtttcataag tcgttccctc aggaaggaaa tgcgagtgat ttcaccgtca
    39301 ccggcacaac cgatccgcca aaaagaggcg taccaatgtc gatatagtcc cccgcgcgga
    39361 caatcacttc gtcaatgact gccaacggga gttgttgtag ctgtgggcgc aacaacatgc
    39421 ccagagcttt accaaagtcc tgcccggcca ccaccagcag gggatgcgga ttcggaaaac
    39481 gcgcgacgaa atcgaccaac gcgttgatga ccgtcagtac cgcggcgtaa cgcacaggca
    39541 gcgaggcggg aagcgccagc acgtacgcgt cagttttggg atcaagatcc agctgaatca
    39601 gcgcctgttg ccaggcactc accagatccg tttcatcaat cgggatcgcc accggcaaat
    39661 tgcgcagcgg cagttgtacg ccctcaagcc agattgtgct gccagagagc gaaagggtat
    39721 gcgcgcccgc gccaatcacc gtggcgcgca cggtttgcgc cggaaactgc acgttcatct
    39781 cacgcaggcg cggatggtca tgcagcgccg ttgccagaag cgggccaata tcggcaaaac
    39841 agaacgggtc ggcgggctgg tggcgataac attcgcccac gccgccagaa agcgtaatga
    39901 tttcgggcgt aacacctgcg ggcagcaaac cggtttgcat taatgcctgc gcgagcggcg
    39961 aaagcgttcc gtcaatcact tcgacaatca gctctgccat ccgccgggta acctgcacca
    40021 gctgcgcgcc ggtcagcgaa cgggcgtcgg tgcctgcgcc gaagcactca tccacaatca
    40081 tctgccccgg tttatgagcg taaaccacgc gcccctggct gtcggtttcc agcaggcgac
    40141 caccgacgtt gaggcaggca gtgccgctga tttttccggc atcgaacagg gcgtagttcg
    40201 cggtgccacc gccgatgtcg atattcagta cccgacacag ccgttgttca gaaagggttt
    40261 gtgccccggc tccgtgaccg gcgatcacgg attcgaggtg cggcccggcg ctggcaacga
    40321 caaaatcgcc cagcgactga gagagcgcca tcaccgccgg gcgagcattg cgggttttcg
    40381 cgctttcacc ggtgatgatg atggcaccag aatcaacact ttccggcgca atgcccgcag
    40441 cctgatattg ctctaaaatc agcgatttca gttccgcttc ttttaaacca ccctgtttat
    40501 cgacaggggt aaagaacacc gggctttgcc agctaatttc gcgtttgatg aattcgtagc
    40561 gcggcacctg cgacaccgcc gcacggttaa ccagttccag gtgggagaaa atcacctggg
    40621 tggtggtagt gccgatatcg ataccgacgc tcagtagctg gcgagtgttc acgattgtgc
    40681 ctccgcttcg gttttagtcg cggtcgcgtc ttcttttggc accagcatca tcgccacgcc
    40741 aatcgccgtt acgccgccga tcaacttgcc gacaatcatc gggaagatca tggcgttcat
    40801 gttggcagcg gcgaagccta agtggtcgcc cagggcgaaa gcagcggaaa cggcgaaggc
    40861 gcagttgatg actttgccgc gggtatccat ctgcttcatc atgccgaaca tcgggatgtt
    40921 gttggcaagc gttgccacca tgccggctgc cgcgatgttg ttcatattca gcactttacc
    40981 gacgctcatc agcggttttt caaaccagcg ggtcagcagc agcaccatcg gatacgcccc
    41041 taacagaacg caggagatag aaccgataac ttcaatggcg cgcatcacct caccgggttt
    41101 atcgccaggg gccataaaga taggatccag accggggatc agttcccagc caagcaggaa
    41161 tttcactacc gcagcggcaa gaccgagggt gatcaatgca acgagaaatt tggcgaagat
    41221 ctggaagccg ttgatcattt tttccgggat gaatttcagc cccagcgcca ccagaatcgc
    41281 aacaatgatc accgggatca tgttcatcag gatcagggcg aaagtgaatt ccaccggctg
    41341 gccgttgatc tgaacaccgg agtacatagc taccagacca ccagcgatac aaccaatcgg
    41401 aatggtcaca atgcccgcca gcacgccgag cgccagataa cgacggtcag aaggttcgat
    41461 aatgccgagc gccaccggaa tggaaaacac aatcgttggc cccatcatcg acccgagaat
    41521 taacccagag tatagccacg ctgctacgtc gccgcccgcc agctctttgg cgaggaagaa
    41581 gccgcccata tcgcacgcca gcagcgttcc ggcgaacatt gacgggtttg cgccgagcat
    41641 ttcgtaaacc gggataatta ccggcccgag tacgtaagcc agtaccggtg ccagcgcggt
    41701 cataccgacc atcgccagac ccagagcgcc cattgccata aagccttctt cgaactgacc
    41761 gcctgatcct tcgatacttt taccgaactt accgaggaaa cgagcagaac cgccgaactg
    41821 cgacaggatc ctgtctacgg cagctatcag cataaagaac atcatgatgt acatgatgat
    41881 ttcgttaatt cccatcgcct ttactccctg ttagttgtta tttattagcg gatgcggcgt
    41941 gaaaacctta tccgccctac atatgcaatc ccgtaggccg gataagacgc ggcaagcgtc
    42001 gcatccggca tttattgcgc cgctgcgtac aggcagacaa tctgctccag gctggcggta
    42061 cgcgggttgc tgcgcagaca aatatcttcc tgcgcggcct gcgcccatgc accgtaatgc
    42121 gcagatgtcg cgccaacatc gcccagtcgt ttaccaatcc caacttccgc aatcagctca
    42181 cttaccgcat taatagcgtc acgatcgtcg gattttttag ttcgcagtgc ccgaccaatc
    42241 tgactaaagc gttcgcgaca aaccatccgg ttaaactcca tcaccgttgg cagcaacata
    42301 gcgttcgcca gaccgtgcgg aatatgcagc gccgcgcccg gctgatgcgc catcgcgtgg
    42361 cacaacccaa gacccgcact ggaaaacgcc attcccgcca tacatgaagc cagcaacatg
    42421 ctctcgcgcg cggcaaggtc gtgaccgtag cccaccgctt tcggcagcga tttgccaatc
    42481 atcgcaatgg caccaatcgc cagactgtcg gtaaacggtg tagcgttcag ggcgctgtat
    42541 gcttcaatgg catgggttaa cgcatcaatg ccggtcatcg ccgtgacatg cgacggcaca
    42601 ccctcggtca atgcggcgtc gaggatcgcc acatccggca tcaacgaggc atgggctaac
    42661 acctgcttgc gcccgctcac cgcgtcgata atcaccgtta cgttggtggt ttcagagccg
    42721 gttccggcag tagtcggaat ggcaatcagc ggcaagcgcg gttgcagaac gctggtttct
    42781 gacatttctg ccagcgtgct atccgggttc gtcaccagca acgccacggc ttttgcggca
    42841 tccagcaccg agccgccgcc aaatgcgatc accccgtcac aacctgactc acgcaactgc
    42901 gccacggctg cacacacgtc ggtaatgcac ggttcgccca ccggacatgg ccagagcgtc
    42961 atggcgatac ctttaacggc caggctacgc gtcagcccgg cggtcatccc cgcctgatgc
    43021 aaaaagctgt ctgccatcac gaacagatgt ttcagcccac gcgtttgcgc ttgctgtccg
    43081 caactgctca ccgcgcccgg accgcaaagc gtcaccggtg gaacgctaaa tgtttttacc
    43141 cgttgcagat tcagggtatc gaacgcctga aagagcgcgg tctgcaattc attttgcata
    43201 tagcccctcc gctttctctc gcccactact ggcgatcgcc agcggggtca taaacaaact
    43261 gtgctgcggt aaatgcacct gtaacgccgg gaattgtttg cgaaacagtt ccgccacgcc
    43321 cggttgcata caagagccgc ccgccagcca taaatcagta atgccctgcc cttcaatatg
    43381 ccgggcgaca atgtccgcca ttttttcgta caccggtttc accgtaggcc aaatctcttc
    43441 accgtgaccg cgcttgtact gctccgcctc ttccagcgaa atacggcgat ttccggcgag
    43501 agtcagagag atgtgatgtc cgccggttgc ttcatccgcc gagtacgtca ccttgccctt
    43561 tttcacgatg gcaatgccgg tagtgccgcc gccgatatcc actacaccgg cgttgtccag
    43621 ttgcagcaga tccgcaactg cggttggctc atccagcaca tggctcactt ccagtccggc
    43681 agattccagc acgttgatgg aaatacgcgg gtccgtacct ggaggaaatg aagtcgccgc
    43741 atggctaaaa cgacgaccga attgctgctc gagcgtgtcg agatggcgac gaacaatggt
    43801 gacagcgccg aagaaatccc agacgatgcc gtcgcgaacc acatcggccc agtcgaggca
    43861 taccgccact ggctgaccgt cgcggtcgac aaccatcgac accacatcgc aggtgcccag
    43921 atccaccccc agccacagcg gggattccgt cgcggcaggc gtctggttac acagcgttgc
    43981 cgctgtttgc agacgtgggg tgagccattg ttcgtcgtgc gccatcggtt actccttaaa
    44041 caatgcgaaa cgcatcgact aatacacagc gacgcagacg gacaaacgtc cgcgcgctgg
    44101 ttaccccttc accggttggc gtggtgatgg tc
//
LOCUS       DAZUCE010000013        44101 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZUCE010000013 DAZUCE010000000
VERSION     DAZUCE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.013"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            325..699
                     /gene="tomB"
                     /locus_tag="ACHY03_000661"
     CDS             325..699
                     /gene="tomB"
                     /locus_tag="ACHY03_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFS3903932.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            725..943
                     /gene="hha"
                     /locus_tag="ACHY03_000662"
     CDS             725..943
                     /gene="hha"
                     /locus_tag="ACHY03_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFS3903933.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            1115..1666
                     /gene="maa"
                     /locus_tag="ACHY03_000663"
     CDS             1115..1666
                     /gene="maa"
                     /locus_tag="ACHY03_000663"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFS3903934.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            1782..2252
                     /gene="ylaC"
                     /locus_tag="ACHY03_000664"
     CDS             1782..2252
                     /gene="ylaC"
                     /locus_tag="ACHY03_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFS3903935.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKCELSFYDIFTLARAESTS"
     gene            2416..3966
                     /gene="pdeB"
                     /locus_tag="ACHY03_000665"
     CDS             2416..3966
                     /gene="pdeB"
                     /locus_tag="ACHY03_000665"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFS3903936.1"
                     /translation="MRTRHLVGLISGVLILSVLLPIGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGADCSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(4008..4361)
                     /gene="ybaA"
                     /locus_tag="ACHY03_000666"
     CDS             complement(4008..4361)
                     /gene="ybaA"
                     /locus_tag="ACHY03_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFS3903937.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4481..4577)
                     /gene="ffs"
                     /locus_tag="ACHY03_000667"
     ncRNA           complement(4481..4577)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHY03_000667"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4740..5051
                     /locus_tag="ACHY03_000668"
     CDS             4740..5051
                     /locus_tag="ACHY03_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFS3903938.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(5082..5654)
                     /gene="ybaY"
                     /locus_tag="ACHY03_000669"
     CDS             complement(5082..5654)
                     /gene="ybaY"
                     /locus_tag="ACHY03_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFS3903939.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5872..6732
                     /gene="tesB"
                     /locus_tag="ACHY03_000670"
     CDS             5872..6732
                     /gene="tesB"
                     /locus_tag="ACHY03_000670"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFS3903940.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKKMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6781..8067)
                     /gene="amtB"
                     /locus_tag="ACHY03_000671"
     CDS             complement(6781..8067)
                     /gene="amtB"
                     /locus_tag="ACHY03_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFS3903941.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(8097..8435)
                     /gene="glnK"
                     /locus_tag="ACHY03_000672"
     CDS             complement(8097..8435)
                     /gene="glnK"
                     /locus_tag="ACHY03_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFS3903942.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(8616..10397)
                     /gene="mdlB"
                     /locus_tag="ACHY03_000673"
     CDS             complement(8616..10397)
                     /gene="mdlB"
                     /locus_tag="ACHY03_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFS3903943.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIALLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10390..12162)
                     /gene="mdlA"
                     /locus_tag="ACHY03_000674"
     CDS             complement(10390..12162)
                     /gene="mdlA"
                     /locus_tag="ACHY03_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFS3903944.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFMYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     YQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(12192..12650)
                     /gene="decR"
                     /locus_tag="ACHY03_000675"
     CDS             complement(12192..12650)
                     /gene="decR"
                     /locus_tag="ACHY03_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFS3903945.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(12804..13622)
                     /gene="cof"
                     /locus_tag="ACHY03_000676"
     CDS             complement(12804..13622)
                     /gene="cof"
                     /locus_tag="ACHY03_000676"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFS3903946.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERNITLTFATGR
                     HALEMQHILGAISLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGASLTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            13722..15422
                     /gene="ybaE"
                     /locus_tag="ACHY03_000677"
     CDS             13722..15422
                     /gene="ybaE"
                     /locus_tag="ACHY03_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFS3903947.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWLNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15487..16182
                     /gene="queC"
                     /locus_tag="ACHY03_000678"
     CDS             15487..16182
                     /gene="queC"
                     /locus_tag="ACHY03_000678"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFS3903948.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(16234..16632)
                     /gene="fadM"
                     /locus_tag="ACHY03_000679"
     CDS             complement(16234..16632)
                     /gene="fadM"
                     /locus_tag="ACHY03_000679"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFS3903949.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSESFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(16738..17109)
                     /gene="ybaV"
                     /locus_tag="ACHY03_000680"
     CDS             complement(16738..17109)
                     /gene="ybaV"
                     /locus_tag="ACHY03_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFS3903950.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(17260..19131)
                     /gene="ppiD"
                     /locus_tag="ACHY03_000681"
     CDS             complement(17260..19131)
                     /gene="ppiD"
                     /locus_tag="ACHY03_000681"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS3903951.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAESMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(19323..19613)
                     /gene="hupB"
                     /locus_tag="ACHY03_000682"
     CDS             complement(19323..19613)
                     /gene="hupB"
                     /locus_tag="ACHY03_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFS3903952.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(19804..22158)
                     /gene="lon"
                     /locus_tag="ACHY03_000683"
     CDS             complement(19804..22158)
                     /gene="lon"
                     /locus_tag="ACHY03_000683"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFS3903953.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(22346..23620)
                     /gene="clpX"
                     /locus_tag="ACHY03_000684"
     CDS             complement(22346..23620)
                     /gene="clpX"
                     /locus_tag="ACHY03_000684"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFS3903954.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(23746..24369)
                     /gene="clpP"
                     /locus_tag="ACHY03_000685"
     CDS             complement(23746..24369)
                     /gene="clpP"
                     /locus_tag="ACHY03_000685"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFS3903955.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(24615..25913)
                     /gene="tig"
                     /locus_tag="ACHY03_000686"
     CDS             complement(24615..25913)
                     /gene="tig"
                     /locus_tag="ACHY03_000686"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS3903956.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(26257..26574)
                     /gene="bolA"
                     /locus_tag="ACHY03_000687"
     CDS             complement(26257..26574)
                     /gene="bolA"
                     /locus_tag="ACHY03_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFS3903957.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            26879..27457
                     /gene="yajG"
                     /locus_tag="ACHY03_000688"
     CDS             26879..27457
                     /gene="yajG"
                     /locus_tag="ACHY03_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS3903958.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            27501..28976
                     /gene="ampG"
                     /locus_tag="ACHY03_000689"
     CDS             27501..28976
                     /gene="ampG"
                     /locus_tag="ACHY03_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFS3903959.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            29436..30383
                     /gene="cyoA"
                     /locus_tag="ACHY03_000690"
     CDS             29436..30383
                     /gene="cyoA"
                     /locus_tag="ACHY03_000690"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFS3903960.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            30405..32396
                     /gene="cyoB"
                     /locus_tag="ACHY03_000691"
     CDS             30405..32396
                     /gene="cyoB"
                     /locus_tag="ACHY03_000691"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFS3903961.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVMIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            32386..33000
                     /gene="cyoC"
                     /locus_tag="ACHY03_000692"
     CDS             32386..33000
                     /gene="cyoC"
                     /locus_tag="ACHY03_000692"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFS3903962.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            33000..33329
                     /gene="cyoD"
                     /locus_tag="ACHY03_000693"
     CDS             33000..33329
                     /gene="cyoD"
                     /locus_tag="ACHY03_000693"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFS3903963.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            33341..34231
                     /gene="cyoE"
                     /locus_tag="ACHY03_000694"
     CDS             33341..34231
                     /gene="cyoE"
                     /locus_tag="ACHY03_000694"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFS3903964.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            34380..35744
                     /gene="yajR"
                     /locus_tag="ACHY03_000695"
     CDS             34380..35744
                     /gene="yajR"
                     /locus_tag="ACHY03_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3903965.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETAGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(35873..36364)
                     /gene="yajQ"
                     /locus_tag="ACHY03_000696"
     CDS             complement(35873..36364)
                     /gene="yajQ"
                     /locus_tag="ACHY03_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFS3903966.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            36532..37443
                     /gene="panE"
                     /locus_tag="ACHY03_000697"
     CDS             36532..37443
                     /gene="panE"
                     /locus_tag="ACHY03_000697"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFS3903967.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIFNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            37406..37996
                     /gene="yajL"
                     /locus_tag="ACHY03_000698"
     CDS             37406..37996
                     /gene="yajL"
                     /locus_tag="ACHY03_000698"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFS3903968.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLMVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(38050..39498)
                     /gene="thiI"
                     /locus_tag="ACHY03_000699"
     CDS             complement(38050..39498)
                     /gene="thiI"
                     /locus_tag="ACHY03_000699"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFS3903969.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            39704..39946
                     /gene="xseB"
                     /locus_tag="ACHY03_000700"
     CDS             39704..39946
                     /gene="xseB"
                     /locus_tag="ACHY03_000700"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFS3903970.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            39946..40845
                     /gene="ispA"
                     /locus_tag="ACHY03_000701"
     CDS             39946..40845
                     /gene="ispA"
                     /locus_tag="ACHY03_000701"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFS3903971.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKK
                     LAEQSLDTSALEALADYIIQRNK"
     gene            40870..42732
                     /gene="dxs"
                     /locus_tag="ACHY03_000702"
     CDS             40870..42732
                     /gene="dxs"
                     /locus_tag="ACHY03_000702"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFS3903972.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRLMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKSIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            42787..43761
                     /gene="yajO"
                     /locus_tag="ACHY03_000703"
     CDS             42787..43761
                     /gene="yajO"
                     /locus_tag="ACHY03_000703"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFS3903973.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            43992..>44101
                     /locus_tag="ACHY03_000704"
     CDS             43992..>44101
                     /locus_tag="ACHY03_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3903974.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERL"
BASE COUNT        10424 a        11687 c        10928 g        11062 t
ORIGIN      
        1 gtaattcttt attcattcct cggttattac gtcatattca gagcaatcct ggtattagtg
       61 tcaccaattt catctggcga taatcctgaa atgttatgaa cagttcgagc aaactgcttt
      121 tacctgctgc gggttggtgc tagtatgaaa aagtgagccc tgtcccgctt ccttcctaat
      181 tgtaattttt cgtaataatg cgatgaaaac ctgcaaagag tggcttatag ttaagctaac
      241 aaacgagagg gcaagtccag gtcagtaagt tttttccatc ccgaaaggtg tccgttagtt
      301 caaccgctaa gaaggggacg cgttatggat gaatactcac ccaaaagaca tgatatcgca
      361 cagcttaagt ttctctgtga aactctgtat catgactgcc ttgcaaacct tgaagaaagc
      421 aatcatggct gggttaacga cccaacctcg gcgatcaacc tccagttgaa tgaactgatt
      481 gagcatattg cgaccttcgc acttaattac aaaattaagt ataatgaaga caataagctc
      541 attgagcaga tcgacgaata tctggatgac acctttatgt tgttcagcag ttatggtatt
      601 aatatgcagg atcttcagaa atggcggaag tcaggtaatc gactattccg ttgttttgtc
      661 aatgcgacga aagagaatcc tgcgagttta tcttgttaga attattacaa ccatgggtag
      721 aagtatgtcc gaaaaacctt taacgaaaac cgattattta atgcgtttac gtcgttgcca
      781 gacaattgac acgctggagc gtgttatcga gaaaaataaa tacgaattat cagataatga
      841 actggcggta ttttactcag ccgcagatca ccgcctcgcc gaattgacca tgaataaact
      901 gtacgacaag atcccttcct cagtatggaa atttattcgc taataaaaaa ttcgctttcg
      961 gagctataac cggctgttta ttaagaattt tatacttttt cgccatgaag acatacccta
     1021 tgtgatcttt atcacacaga tgtaatggga acgttctctt cactgacttt tcgtcttact
     1081 gtgttgccgc attttcagca accggaggtc agtaatgagc acagaaaaag aaaagatgat
     1141 tgctggtgag ttgtatcgct cggcagatga gacgttatct cgcgatcgcc tgcgcgctcg
     1201 tcagcttatt caccgataca atcattccct ggcggaagag cacacattac gccagcaaat
     1261 tctcgctgat ctattcggtc aggtgacaga ggcttatatt gagccaacgt ttcgctgtga
     1321 ctatggctat aacatttttc tcggtaataa ttttttcgcc aacttcgatt gcgtgatgct
     1381 tgatgtctgc cctattcgca tcggtgataa ctgtatgttg gcaccaggcg ttcatatcta
     1441 cacggcaaca catcccatcg accctgtagc acgtaatagc ggtgctgaac tggggaaacc
     1501 cgtcaccatc ggtaataacg tctggattgg cggacgcgcg gtcattaacc ctggtgtgac
     1561 cattggtgat aacgtcgtgg tagcctcagg tgcagttgtc acaaaagatg tcccggacaa
     1621 cgttgtcgtg ggcggtaatc cagccagaat aattaaaaaa ttgtaatcgg tttttcgcaa
     1681 ctgttatgca aaattgtggt aaatctgtta ctttccctct actattccca cgttaaaata
     1741 gggtgttccc tggaaagttg cagataccac gaaggcaaac gatgaccgaa atacaacgcc
     1801 tgctgaccga aacgattgag tctctgaata cccgcgaaaa acgcgacaac aaaccccgct
     1861 ttagtatcag ttttatccgt aaacatccgg ggctgtttat cggtatgtac gttgcttttt
     1921 ttgccaccct ggcggtgatg ttgcagtccg aaacgctttc aggctctgtc tggctactgg
     1981 ttgtattatt tatcctgctt aatggtttct tctttttcga tgtctaccca cgctaccgct
     2041 atgaagatat cgacgtgctg gatttccgcg tttgctataa cggcgaatgg tacaacacgc
     2101 gctttgtacc tgccgcgctg gttgaagcca tcttgaactc tccgcgtgtc gcggatgttc
     2161 ataaggaaca actgcaaaaa atgatcgtcc gtaaatgtga actgtctttt tacgatattt
     2221 ttaccctcgc tcgcgccgaa tcaacatctt aagttaggat tacataccag gcgtaaagct
     2281 ctgcgcctgg tcaaatgaca acgatcgttt ccacccatca cttcatgaaa taccagctct
     2341 acctccttat ctcctgccag cctttttcca caatcagata tactttccct acactgtgtt
     2401 aataaggata tgctggtgag aacacgacat ctggtcggtc ttatttcggg agtactgatt
     2461 ctttcagtat tgctgcctat cggcttaagc atctggctgg cccatcagca ggtagaaaca
     2521 tcgtttattg aagagctgga tacctattcc tcccgcgtcg ctattcgggc caataaggtg
     2581 gcgacacaag ggaaagatgc gctgcaagag ctggaaagat ggcaaggcgc tgactgtagt
     2641 gaagcccatc tcatggaaat gcgtcgggta tcttacagtt atcgctatat tcaggaagtg
     2701 gtttatatcg ataacaacgt tccccaatgt tcgtctctgg agcatgaaag tccgcccgat
     2761 accttccccg agccaggtaa aatttcgaaa gatggttatc gtgtctggtt aacatcacat
     2821 aacgatttag gcattatccg ttacatggtc gccatgggaa cggcacatta tgtcgtcatg
     2881 atcgaccccg cttcctttat tgatgtcatt ccctatagct catggcaaat tgatgccgcc
     2941 attattggca atgcccataa cgttgtcata accagcagcg atgaaattgc tcagggaatt
     3001 attaccaggc tacaaaaaac acccggtgag catatcgaaa ataatggaat catttacgat
     3061 atcctgccct tcccggagat gaatatttca attatcacct gggcttcaac gaaaatgttg
     3121 cagaaaggct ggcatcggca agtctttatt tggttaccgc tcgggttggt gattggcctg
     3181 ctggcagcga tgtttgtgct gcgtattttg cgccgtatcc agtcaccgca tcatcggctg
     3241 caggatgcta tcgaaaatcg tgatatttgc gtgcactatc agccgattgt ctccttagcc
     3301 aatggcaaaa ttgtcggtgc tgaggcactg gcgcgctggc cgcagacaga cggtagttgg
     3361 ttgtcaccag atagttttat tccgctggca cagcaaacgg gcctttctga gccattgacg
     3421 ctactgatta taagaagcgt ctttgaagat atgggcgact ggctgcgtca gcatccacag
     3481 cagcatattt cgatcaatct tgaatccacc gtgctcacct cggaaaaaat cccgcaattg
     3541 ctgcgtgaaa tgatcaatca ctatcaggtt aatcccagac agatcgcgct tgaactcact
     3601 gaacgcgagt ttgccgatcc gaaaaccagc gccccgataa tttctcgcta ccgggaggcg
     3661 ggccatgaaa tttatcttga tgattttggt acggggtatt caagtttaag ttatttacag
     3721 gatctggatg tcgacattct gaagatcgat aaatctttcg ttgatgcgct ggaatataaa
     3781 aatgtcacgc cgcatatcat cgaaatggca aaaacactga aactgaaaat ggtagcggag
     3841 ggaatcgaaa ccagtaaaca agaagagtgg ctacgccagc atggcgtgca ctacggtcag
     3901 ggctggctct acagcaaggc attaccgaaa gaagatttct tacgctgggc cgagcaacat
     3961 ttgtgaattt ttgggctgac ggcacgccgc acagcccgtc acgctaccta ttcgtcgatg
     4021 attgactcga atccgccata gatcattcgc ttgccgtcaa acggcatgga ctcgccgaac
     4081 tctttcatcc gtgggtccga catcatcttt tgattagctg cgtcgcggac ctcttttgaa
     4141 gggtattcaa tccagctaaa gacaacctct tcattctctt ccgctttcac cgccatacga
     4201 aaatcggtca ctttgccatc cggtacatcg ctggcccagc attcgacaat acgaagcgcg
     4261 ccgaactctt taaataatgg cgcagcctta gcggccattt cccgataggc atccttttta
     4321 tcggcaggaa cggcaaccac aaaaccatca acatacttca tcagacatac ctccgaagcg
     4381 tactgcgcag ccagacaatt cttgcgacgt cagttgtggt taaagtttag tcacgctggg
     4441 attttttgac gaaacggtgg gaagcagaaa tgggtggggg ccctgccagc tacatcccgg
     4501 cacacgcgtc atctgccttg gctgcttcct tccggacctg acctggtaaa cagagtagcg
     4561 ttgcgggaga accaacagag cccccattga gagcgttgag aaccaacgcg caggcgcatt
     4621 atcactgctg ccccggctaa ttgcaaccca ttgcgtgcca gatgctggtt tcttgcgcaa
     4681 tgcgacttca ctcgggcgtt tttccagatt atgctgaaaa attacctcag gaagaaaaga
     4741 tggaaaaaga agattcattt ccccaacgcg tctggcaaat cgtcgccgct attcccgaag
     4801 gctatgtaac cacttacggt gatgtggcga aactggcggg atcgccccgc gccgcgcgcc
     4861 aggtgggcgg tgtgttaaag cgtctccctg aaggcagcac cttaccctgg caccgggtgg
     4921 ttaatcgcca cggcacaatt tcgctaaccg gaccggattt acagcgtcag cgacaggcat
     4981 tactggcaga aggtgtgatg gtatcgggaa gcgggcaaat cgacttgcag cgttatcgct
     5041 ggaactacta accctctccg ggcggagagg gttaacgtcg cttaatattg cgtaggagct
     5101 ggaactgccg aagacggatt cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg
     5161 ccaccgctgg cctgaaccgg cacggcagtt tgctgtaccg gcaccaatgt caggtcggct
     5221 ttagttccgc cctggttgat caccggctga acggtatcgg tgataaatac cagtttgtca
     5281 ttcacggtaa tcgccgcact caacagaata cgcgcgttcg gctgaacatc tgccgggtta
     5341 aatggcagaa caaagctgaa tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc
     5401 agcacttttg acggtgcatc ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca
     5461 tcaggcggca gtgcgacttt ctgacggatc cagacggtac cggagacatt cggttgctgg
     5521 atagcaggtt gttgtgttgc tgaaatagac gtatttgcag ccggggctgg cgtctgaata
     5581 tccgcgcttt tatctgcgca agccgccaac gcaatcgcaa ccgctaaacc actggccatg
     5641 tgcacgagtt tcattcattt ctccttatta tcaatgcacc agcgggctaa ctttcctcgc
     5701 cggaagagtg gttaacaaaa tagtaacgtc aacaagtgtg gcacacatca cgcatttctg
     5761 cctgtaatta gcccgtaatt cagaccattg cacccatcgg accagtagca aatttgcgtt
     5821 atactcaact cactttggct tgctgcggca gctttgttac tggagagtta tatgagtcag
     5881 gcgctaaaaa atttactgac attgttaaat ctggaaaaaa ttgaggaagg actctttcgc
     5941 ggccagagtg aagatttagg tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc
     6001 ttgtatgctg caaaagagac cgtccctgaa gagcggctgg tacattcgtt tcacagctac
     6061 tttcttcgcc ctggcgatag taagaagccg attatttatg atgtcgaaac gctgcgtgac
     6121 ggtaacagct tcagcgcccg ccgggttgct gctattcaaa acggcaaacc gattttttat
     6181 atgacagcct ctttccaggc accagaagcg ggtttcgaac atcaaaaaaa aatgccgtcc
     6241 gcgccagcgc ctgatggcct gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg
     6301 ctgccgccag tgctgaaaga taaattcatc tgcgatcgtc ccctggaagt ccgtccggtg
     6361 gagtttcata acccactgaa aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc
     6421 gcaaatggta gcgtgccgga tgacctgcgc gttcatcagt atctgctcgg ttacgcttct
     6481 gatcttaact tcctgccggt agctctacag ccgcacggca tcggttttct cgaaccgggg
     6541 attcagattg ccaccattga ccattccatg tggttccatc gcccgtttaa tttgaatgaa
     6601 tggctgctgt atagcgtgga gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt
     6661 gagttttata cccaggacgg cgtgctggtt gcctcgaccg ttcaggaagg ggtgatgcgt
     6721 aatcacaatt aaaaaaacag ccggaggtga aaaccgtccg gctgtttttt gcagtgcttg
     6781 ttacgcgtta taggcattct cgccgtggct gttgacatcc agcccttctc gctcctgctc
     6841 ttccggtaca cgcagaccaa ccgtcagatc cgccagtttg tagccaataa atgccaccac
     6901 accggaccag acgatcgtaa tggcgatgct ttccaactgt accagcaact gatggcccat
     6961 cgtcacacct tcagcgaagc ccacgccgcc cagcgagctg gcggcaaaaa tcccggtcat
     7021 gatacagccg acaatgccac aaacgccgtg cacaccgaag acatcgcagg gatcatccac
     7081 ccgcagcaag cgtttgagca tggtaacgcc ccacaagccc gccagaccag ctaccacgcc
     7141 gataatcaac gcgccgccaa caccaatgta gccacaggct ggcgtcacgc cgaccagacc
     7201 ggcaatcgcg ccagaacacg cccccagcag tgaaggctta ccacgcagcg cccattcacc
     7261 gaagatccag ccaagaattg ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc
     7321 cgcgatttca ttcgccgtgc ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag
     7381 aatggcagtc ccggtgaaga ccatcggcag gttgtgcggt ttaaacgcct ctttgccgaa
     7441 gcccacgcgt ttgcctatca gatacgcgcc caccagaccg gcgattgcgg cgttaatatg
     7501 caccaccgtg ccacccgcga aatccagcgc accgtgagaa gccagcaaac caccgcccca
     7561 caccatatgc gcaatcggaa tgtaagagag cgtcagccat accaccacga aaatcaacac
     7621 agctgagaag cggattcgtt ccgccagcgc cccaactatc aagcctacgg taatgcaggc
     7681 aaacgatccc tgaaacgcta cgtggatata ctgataaatg ctgcccatca ccgccgtcag
     7741 ttcgatgttt ttcagcatca accagttaat gttgccgaag aagttgttgc cctcaccaaa
     7801 cgccagcgag taaccgtaaa ccacccagag aatacagacc agtgcaaatg tcaccgtcac
     7861 ctgcgtcagc atcgacagca cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat
     7921 ccccggaata gtcataaaca gcaccagcgc agtacaaatc atcataaacg cattgtcggc
     7981 tttatcggcc accgcaggtg cagccattac cagtcccggc agcatcgcca gtgaagcaag
     8041 cccagttttt atcgtcgcta tcttcatttt tcgttcctgt tgctgtgtgc cagagattac
     8101 agcgccgctt cgtcggcttc gccggtacga atacgaatga cgcgttgcaa ttcagcgacg
     8161 aagattttgc cgtcgccaat ttttccggtg taagccgcct tactgacgat atcgatcact
     8221 tcatcgagtt ggtcatcagc aatcgccaca tcaattttta cttttggcag gaagttgaca
     8281 ctgtattccg ccccccggta cagctcggca tgccctttct gacgcccgaa acctttcact
     8341 tcggtgacgg tcaggccctg aataccaatg gaagataacg cttcacgaac gtcttccagc
     8401 ttgaatggtt tgattatcac ggtcaccagc ttcatagatc ccctccggtc agaattcggt
     8461 aatggttctg ctacacgaag aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa
     8521 agagagcagg aagttaagaa gtttgaaaat gaaaacgcac tatgacagtg cacagtgcgt
     8581 tacatttttg caccaacgat gaaatattgc gctattcagg cgctcaatga ttcctcttca
     8641 cgcacgcttg ctgccagctc ttcgcccgca agttgcagtt gatacatctg ccagtagcgt
     8701 ccttgggccg ccagcagttg ctggtgagtg ccctgctcca cggcttgccc acgatgaagc
     8761 accagaatgg tgtcggcatc gacaattgtc gataagcggt gagcaatcac caccagcgtg
     8821 gtatgttcac gcaccgctgc cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg
     8881 ctggcggttg cctcatcaag gatcaggatt tgcggcgtct ccaccaacac gcgtgccagt
     8941 gccagcagtt gcttttgccc gacagagaga ttattcccct gctcgcccag cggcgtgtaa
     9001 ataccgtcgc tcatgctacg cgccagctcc gccagttgca cggtttccag cgcctgccag
     9061 acgcgttctt cggagatatc acgccccagc gtcacgttgg cgaggaaggt atccgccagc
     9121 accaccggat cttgctgcac cattgccacg ccctggcgca gcgcgctgtg acttagcgaa
     9181 cttaatggac gaccatcaag gcgaatctca ccttccgtta gcgggtaata gcccatcaat
     9241 aaactggcga gggtactttt accactgccg gtatgcccga ccagcgccac aaagttacgc
     9301 gaaggcacag agagattaat gttctttagc accagattgt catcgcgata agcaaatgac
     9361 acgttatcga cttcgatggt gccgctctgt aacgggcgat catcattgcc atattgctgg
     9421 cgcggtccgt ccatcagctc aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc
     9481 gcctgctgcg tggtcagttc gattaagggt tcgttaagtc gcccaagata gctgataaac
     9541 gcatacaaca cgcccacttc aatggtgccg ctggcggaga agccaaacag catcaacaag
     9601 ccacagagaa tgagcgatga aaacagactc agcagcggac gcagcagaaa accgtcgagg
     9661 cgcagggttt gcatcctcgc catatagtgt gaacgactgg cctcacccat gcgttcgcca
     9721 aatcgcgcct gctggcggaa ctgctggata acgctcatgc cattgatgat ttcgttaaag
     9781 ccgtcgttga tatccgccag ataggcgcgc acacgacgga caatcggcgt actgtaacgc
     9841 tggtatatca ccattaccac cagcaccacc gggaaaatca ttatcgccac cagcgccatt
     9901 cgccagtcga ggctgaacat cgccaccagc atcgcgccca ccagtgcggc actgcgcagg
     9961 acagttgcca ctacggtaac gtagagatcg cggatcactt cagtgtcatt agtgacgcgg
    10021 gaaatcacct gcccgacggg ttgggtatca aactcgctta atggctggcg taacgcagca
    10081 tccatcacgt cggtacgcaa ctgttgcact acgcctactg ccgcccgatt aaacagcagc
    10141 gattgcgcgt aatgtagccc ggcggcaaac agttgtagcc caacatacgc cgcagccagc
    10201 cctgcaacca ctttcaacgg caggttattt ttcgctacca tattgtcgat aaaatagctg
    10261 ataagcagcg gcccactgac ttctgccgct gccgcaaccc acatcatcag gagcgcaatc
    10321 cccagcggtt tacgccacgg cgaaccgtac gctaacagac gcttgagagt cggccacagt
    10381 tggctaaaac tacgcatcga cggcctcctc gcgaatttcc ggagcgtcgt cgagcgccgc
    10441 ctccagttgt tgatagcgat acatatcgcg ataccagccg ctttgttgtg ccagctcatc
    10501 atgattgcca cgctgggcga tatgtccgtg ctgcatcaca ataatttcac tggcttccgt
    10561 cagtgccgaa aggcgatggg cactgatgat taccgttctt ccctgtcccc actggcgcag
    10621 gttatgcagg atctggtact cagtgcgtcc gtccaccgcc gaaagcgcat catcaaggat
    10681 gaggatttcc gcgttgacta ataacgcacg agcaatggag atacgctgtt tttgcccgcc
    10741 ggaaagcatc acaccgcgct cgcccacctc tgtatcgtaa ccttgcggta gacgcaaaat
    10801 atcgtcatgt acgctggcta accgcgcgac atgctcaatc tcttgctggg tggcattcgg
    10861 gcaacccagc gcgatgttat tcgccacggt gtcagaaaaa aggaatggcg tctggctaac
    10921 taccgccagg cggctacgcc agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa
    10981 gcgaatatcc ccctcgctga cgtcgaaatg acgctgaatg agcgacaaca gggtactttt
    11041 gccggaacca gtcggcccgc agatacccag catctgaccg ggtttcaggg cgaaattgac
    11101 gttttccagc gcaggatggt cagtctgcgg atacatgaac tggtgaatat ttacatccag
    11161 ttcgccacgc ccttccggca cgggttcgct accatcgttc accaccggcg cttccgccag
    11221 catcgcgcga atacggctgt acgcagcact accacgttcc acaatgttaa acatccatgc
    11281 cagcgccagc attggccaaa tcatcagacc taaatacatc ataaaactgg tgagctggcc
    11341 cagcgttaaa ctgccctgca ccaccatcca actaccgccg ccaatcgcca gcaagttcgc
    11401 cataccaatc gcgatataga tggtcgggtc gaaacgagca tcaatacgcg ccacccgcat
    11461 gttttttttg ccggtatctt cggcatccgc ggcaaataac gccgactggc gatcttccag
    11521 accaaaggct ttgatcatgc ggatactggt gaggctttcc tgggtgcggt cattaagact
    11581 ggaaaacgcc gcctgtgcca gcttaaagcg ttcatgcaaa gcatcgccgt tgcgcttaat
    11641 catgatcgcc atcactggca tcggtaacag ggcaaataag gtcaactgcc agctaatttg
    11701 cgtagacatc ataatcaaca cagcgcagcc cattaccagt gaatccacca gcgtcagcac
    11761 cccttctccg gcggcaaaca cgacgcgatc gacgtcatta gtcgcacgag ccatgaggtc
    11821 acccgtgcga tgacgcaggt aaaactcagg atgctgccgg cttagctgac ggtaataatc
    11881 ttcacgcagt tcaacagcca gttgataaga cgcaccaaac agcaatacgc gccagacgta
    11941 acgcaggaga taaaccacaa cggcaatcag caccatggtg gcgatccaca tcaggatctg
    12001 cccggtagta aagtgttgtt ctgtcacgcc atcgacaaca ataccaacca cttttggtgg
    12061 aaccagttgc agcatcgcga taatgacaag caaggcgaca gccccgagat agcgacgcca
    12121 ttcccgacgg aaataccagc ttaattgagc aaataatcgc acgcgttgtg tcctgacctg
    12181 attctggata tttattcgat gggtaaagaa gtggtgtatt taatctgttc catcgcgaag
    12241 ctggaagtga cgtccgacag ccccggcacg ctgtttacca gacgcttata aaactcgtcg
    12301 tagcgtttca tgtcggcaac ctggacgcgc atcagataat cgtattcacc agccatgcgc
    12361 cagaacccca gcacttctgg catttcggta accaccgtga caaagcggca ataccattcg
    12421 ctgctgtgat gttgcgtttt tatcagcaca aaggcggtca gaccgaggcc tattttttcc
    12481 ggatccagca gggcgacttt gccgataagg ataccgtcgt cctccagccg tttcaggcgc
    12541 ttccagcaag gggtggttgt cagattaacg gcttcagcca gtgcctgcaa agagagggtg
    12601 caatcctgct gcagtaaggc cagcagctta cggtcaattt tatctaacat agcccttcca
    12661 cagagaattt ttttctcttt taattctatt ttaaaggtga aatcgcgaca atttattctg
    12721 tgcggtttaa acaccacggc acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt
    12781 gcccggtgct ggaaggaatc tcgttattcg ggggaataag gtagatgtgg atagtccagc
    12841 cagtgcgtca aatagtgaga gacagcctga tttcggcaat gtccaatcac cggtaaatgc
    12901 gggagctccg cgcgcagttg cggcatcgca ttgcccataa taaatccgct accgacgctg
    12961 cctaacattt cgcgatcgtt catcgcatca ccaaaggcca tgcaatcgcg caacgataaa
    13021 cctaaatgtt gggtcagtac cgtcaatgaa gcgcctttat tgcagcccac cggcagcact
    13081 tcaaggcaat ccgtggcgga aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc
    13141 tggatctgca aacgtgtaag atcgtcgtga tcgccacaga agcagatctt ggtgacgctg
    13201 ccgagtggca tttttttgac atcgattatt tgataacgaa aaccgctata gacaaatgcc
    13261 tgcaacaacg cagggatctc tttcccggta aaccaaccgt cgtcattgaa aatatgcatg
    13321 ctggctcggg tatcccattg ctgatacagc accagctccg cgacatccgc aggtaaatca
    13381 tcacgatgta aaagttcacc ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa
    13441 tacgcatcca gcgatatcgc cccgagaata tgctgcatct ccagcgcatg acgccccgtg
    13501 gcaaaagtga gggtaatgtt gcgttcacgc agtcgcgcca aagtagagag ggttttctca
    13561 cctaaatgat ggtcaggcat caataaagtg ccatccatat caaatgctgc cagacgagcc
    13621 atttctttct ccactctgtg acacggttaa tggtgattga gtatcacctg atatatacgg
    13681 aagtaatagt gaatagttaa ataatattgt tccgggttta tatgcgactg ctcaaccgtc
    13741 ttaaccagta tcaacgtctg tggcaacctt ccgccggaaa gccgcaaacc gtcaccgtca
    13801 gcgaactggc cgaacgctgt ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg
    13861 cacaggaggc gggatggctg gagtggcagg cgcagtcagg acgcggaaag cgcggacaat
    13921 tacgctttct ggtcacgcca gaatcgctac gcaatgcgat gatggaaaag gcactggaaa
    13981 ccggaaaaca gcaagatgtg ctggagctgg cgcaactggc cccaggtgag ctgcgcactc
    14041 tgttacagcc gtttatgggc ggacaatggc taaacgatac acccacgttg cgtattccct
    14101 actatcgccc gctcgaaccg ctacaaccgg gctttttgcc cggccgtgcc gagcagcatc
    14161 tcgccggaca gatattttcc ggcctgaccc gcttcgataa taatacccag cgcccgattg
    14221 gcgatttagc gcatcactgg gaaacctcta ctgacggatt acgctgggac ttttatcttc
    14281 gttcaaccct acactggcat aacggcgatg cagtaaaagc ctcacactta caccagcgat
    14341 tattgatgct gttacaactg ccagcactgg atcaattatt tattagcgtg aagcgtattg
    14401 aagtcaccca tccgcagtgt ctgaccttct ttttacatcg ccctgattac tggcttgcac
    14461 accggctggc gagctattgc agccatctgg cgcatccgca attcccactg atcggcacgg
    14521 gtccttttcg cttaacacaa ttcacagcgg agctggtgcg cctggaaagc catgattatt
    14581 accatttacg tcatccgcta cttaaagcgg ttgagtactg gataactccg ccgcttttcg
    14641 aaaaagattt gggaaccagt tgtcggcatc ccgtgcaaat caccatcggc aaaccggagg
    14701 agctgcaacg ggtcagccag gtcagtagtg gcatcagttt aggtttttgc tatttaacgt
    14761 tgcgcaaaag tccccgactc tccctctggc aggcgcgaaa agtgatctcc attattcatc
    14821 aatccggttt attacaaacg ttagaagtcg gagaaaacct gatcaccgcc agtcatgcat
    14881 tactgccagg ctggactatt ccgcattggc aagtaccgga tgaagtcaaa ctaccgaaaa
    14941 ccttgacgct ggtttatcac ctaccgatag aacttcatac catggcagaa cgtctacagg
    15001 cgacactggc agcggaaggc tgcgaactca caattatttt tcataacgca aaaaactggg
    15061 acgacacgac cctactggca cacgcagacc tcatgatggg cgacagatta attggcgaag
    15121 caccggaata taccctggag caatggctgc gttgcgatcc actgtggcca catgttttcg
    15181 acgctccagc atatgcacat ttgcaatcga cactggacgc ggtgcaagta atgcctgatg
    15241 aggaaaaccg atttaatgcc ctgaaagcgg tttttagcca gttaatggca gacgcgacgc
    15301 tgacgccgct attcaactat cactatcgca ttagtgcccc tcccggcgtg aacggtgtgc
    15361 ggctgacacc gcgcggctgg tttgaattta ccgaagcctg gcttcccgcg ccgtcgcaat
    15421 gaatagctgg tccgggttaa tcgcaggcgt taccataacg cttttattat tcaagcagga
    15481 ttatctatga aacgtgctgt cgttgtgttc agtggaggcc aggattccac cacctgtctg
    15541 gtgcaggcat tacaacaata tgatgaagtc cattgcgtga cgttcgatta cggtcagcgc
    15601 catcgcgcag aaatcgacgt ggcacgcgaa ctggcgctga aactgggggc acgcgcgcat
    15661 aaggttcttg atgtaacgct gctcaacgag ctggcggtca gcagcctgac gcgtgacagc
    15721 attccggtgc ctgattatga acctgaagcc gatggcatcc cgaatacgtt tgtcccaggg
    15781 cgtaatattt tgttcctgac gctggcggca atatatgcgt atcaggtaaa agcagaagcc
    15841 gtaattactg gcgtctgcga aacggatttc tccggctacc cggattgccg cgatgagttt
    15901 gtgaaagcac taaaccatgc cgtcagtttg ggcatggcga aagatattcg ttttgaaacg
    15961 ccgctgatgt ggattgataa agcggaaacc tgggcgctgg cagattatta cggcaaactg
    16021 gatttagtcc gtaacgaaac gttgacctgc tataacggca ttaaaggcga cggttgcggt
    16081 cattgtgcgg catgtaattt acgcgccaac ggtttgaatc attatctggc cgataaaccg
    16141 acggtgatgg cagcgatgaa gcagaaaacc gggttgaagt aattattccg ggtgtcgccg
    16201 gatgcggctt gagcatccgg caccacaaaa cgtttactta accatctgct ccagcttttc
    16261 gcgcaattcc ccttccagag ctaatgcttt ctgcgtttta agatcaatac aaacaaacgt
    16321 aataagcgca tccgctacca cctgcccttc cggctccagt gtaatgacct ggcttaagat
    16381 gccgctttta ccgtttaatt gctgcaactg actggtaata gttaacaggt cgcttaatac
    16441 cgccgggcga cgatagttaa tattgatatt gaccacgacg aaggcgatgt tatgggccgt
    16501 catccactga aaactctcac tattttccaa cccatcccag cgggcttctt cgagaaattc
    16561 aaggtagcgg gcgttgttga cgtgctggta aacgtcgaga tgatatccac gaactttgat
    16621 ttgtgtttgc atagcgcaat aaccttacgt tgtttttata aaaactgtta taagtacaga
    16681 ggtcataact ggtatgacct cttcagtctg gcaaaaaatt gccactatgc aaattaatta
    16741 cagggttaat accgccagat tacgttccac cagcgaattg cccatccccg gcacctgctt
    16801 taggtcctcc accgttttaa acggaccgta ctcttcgcga taactgacaa tcgcctgcgc
    16861 tttcttcagg ccaacgccat tcatcgcgcg ggctagctct tccgcgctgg cattattaat
    16921 gctgacccgg gtgccttctt cgtcactggc tttcgccggt actgctgctt tactttgtgc
    16981 tgcaggagct tccgctttgg tttctaccgc cgtcggtttc gccacagaag ctgccgccag
    17041 cgcgctatga gacattccgg cacaggccag ggacagggta atgagcagtg ctttaattcc
    17101 gtgtttcata ctgttttctc cttgtttgtt aacagtggaa tcaccttaac gggagtgaaa
    17161 acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg cgaaagcgac cttttcttgt
    17221 tacagctgca aagcattttt ttgcgaggcg gcttcaggat tattgctgtt ccagcgcatc
    17281 gccaattttg attttcgcct ctttacgcag gttactcatc agagcttcaa agacgatttg
    17341 tgcgttgttc tgggtgatac cctgcaccat cgcttttttc tgatcttccg gcattgaacc
    17401 ttgtttcact tcatccagcg ccagcagaac cacattacct tgcatatcgg tcgccatacc
    17461 gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac gccgcctggc taatcgggtc
    17521 acgaccggaa cggcttaagg ttttcggctc gccaaatttc agaccggcag cctgcataga
    17581 ttccgcacct ttgccggctt tcaaatcaac cagcagtttc tcagcatcca ctttcgcctg
    17641 ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt tcctgaacat ctgccaacgg
    17701 tttcaccgct tccggtttgt gctcgctgat gcgcagcacg aatgcgcggt cgccgtctac
    17761 ggtgatgatg tcagagttga tgcccggcgc gccgttttca cctaccagac cgccgttaaa
    17821 gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc aggttatctt tgctgaacca
    17881 acccgtctga gtggctttaa cgccggcagc ttgctctgca ccggccagag actcggtgtc
    17941 gttgcttgcc gcatcgctca ctttctgctg cagcgcgtag tacgcatcga gggctttttc
    18001 gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct aacgatttca ctttcgctgg
    18061 ctgaatgtcg tccagacgta caatcaggaa accgaccgaa gatttgatga caccagacag
    18121 ttggcctttt tctttcagac cagcattttt cagttcatcc gggatagtgg catcttctaa
    18181 ccaacccata tcgccgccgt tacgagcaga gataatatcg gcagattttt ctttggctaa
    18241 tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc gctttcgctt catcttcagt
    18301 tttggtctgg atgatgctgt agcgggtacg ctgcggctgg gtgaattgat cctgatgctg
    18361 gtcgtagtag ctctggatat ccgcatcgct aaccggttgc tgcatcgttg cggcatccag
    18421 cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa ttgtttttgt tttgttcgta
    18481 gtagctggca atctcctgtt cggtcacagg ctgcttcgcc gccagcgcgt taacatcgat
    18541 agtcgcttca cgtaccacgc gttgttgagc gaccagtgcc gccagctcgt cggtttcacc
    18601 tttcagcata aaatcggtac cggcaacgcc gttaatcagc tgttgggtag tgagctggtt
    18661 acgcagcgcc tgggcgtact gatcggcggt catccccatc tggttgagga taccgttata
    18721 gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg gtcgcgaaaa tcgcctgttt
    18781 aacctgctca tcgctgatac ccagtttcag ctcacgtgcg tactgatcca gcagcgcctc
    18841 gtcgatcaga cgattcagca cctgttgacg cagggttttc atatagcctt cgttcgctgc
    18901 cagctcggag tattgatcgc ccaactgttg ctgcatgcga ttacgctcgc tgttgaaggc
    18961 gttctcgaat tgcccacggc tgatttcctg gtcattcact tttgcggcgt agttattgcc
    19021 tccgccaatc aggtaaccac tcacgccggt caatatgaac gacacgataa tgataccgaa
    19081 aataatcttg agcacgagac tgtttgcagc cgtgcgtaag ctgtccatca tggtgtaaca
    19141 acactccgct gtagatgact gtgtacctca cgcaacgtat cgatgcgcgc aaaggcctat
    19201 tgtgacaaga aacgggggca attgtcagcc cacaagacac agaaagtcac cgaatacaaa
    19261 taaaaaaggc acatcagtag atgcgccctt gaacttcgtc acatccccac tggggacaac
    19321 gcttagttta ccgcgtcttt cagtgcttta cctgcacgga agctcggtac tttagcggca
    19381 gcgatggtga tctctttacc ggtctgcggg ttgcggccag tacgggcagc acgctcttta
    19441 acggcaaaag taccaaaacc taccagtgct acatcatccc cttctttcag agattcagtt
    19501 acggaagcaa taatagcatc taacgcacgg ccagccgcag ctttagagat atcagcccct
    19561 gcagcaatct tgtcgatcaa ttgagattta ttcactcttc tcttcctctt tataatttat
    19621 atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt atatcaggcc agccatcccc
    19681 ttacaacacc ccttaataag ggcaagcccg atccgccatc taacttagcg agacaaaaaa
    19741 aggctggcaa gcccgaatta gcctgccagc cctgttttta ttagtgcatt ttgcgcgagg
    19801 tcactatttt gcagtcacaa cctgcatgcc agacggttca ttttgcagcg ccagagtcag
    19861 aacttcctca atgcgcttca caggatgaat gtccagatcg gcaattacgt tgtcaggaat
    19921 ctcttccaga tcgcgtttat tttcgaacgg aattaacact gttttaatcc cgccgcgatg
    19981 cgctgccagt agtttttctt tcaaaccacc gatcggcagt acctgcccac gcagagtgat
    20041 ctcaccggtc attgccacat cggcacgaac cgggttaccg gtcaggcaag aaaccagcgc
    20101 ggtgcacata gcaataccgg cactcggacc atctttcggc gtcgcacctt ccggtacgtg
    20161 gacgtggatg tcacgttttt cgtaaaaatc agggttgatc cccagttttt ccgcacgcgc
    20221 acgaaccacc gttaacgccg cctgaataga ctcctgcatc acttcgccga gcgaaccggt
    20281 ataggtcagt ttgcctttac ccggaacaca tgcggtttca atggtcagca agtcaccgcc
    20341 cacttcagtc cacgccagac cggttacctg accgacacgg ttttcgttat ccgcgcgtcc
    20401 atagtcaaaa cgctgaacac cgagatagtc atgcaggtta tcgccgttaa tttcgatatg
    20461 ttttaatgac ttatcgagca gtaactgctt aaccgcttta cggcacagtt tggagatttc
    20521 acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa cgaataatgc cgataatggc
    20581 gctatcgtcg acggtcagct cacctttttt cagtgcatta cgttcaatct gcttcggcag
    20641 caggtgacgt ttggcgatgt tcagtttttc atcttcggta taaccggaga ggcgaatcac
    20701 ttccatacga tccagcagcg gtgccggaat gttcatggag ttcgacgtcg cgacaaacat
    20761 cacgtcgctg agatcgtaat ccacttccag gtagtggtcg ctgaacgcta cgttctgctc
    20821 tggatccagc acttcaagca gtgcagaggc cggatcgcct cgcatgtcag aagacatttt
    20881 gtcgatctca tcgagcagga acagcgggtt tttcacgccc actttcgcca ttttctggat
    20941 cagtttaccc ggcatagaac cgatgtaagt acggcggtga ccacggattt ccgcttcatc
    21001 acgcacgccg cccagcgcca tacggacata tttacgacca gtggctttgg cgatggactg
    21061 acccagggac gttttaccta cccccggcgg ccctaccagg cagaggatcg gtcccttgat
    21121 tttgttgaca cggctttgaa ccgcaagata ctcaaggatt cgatctttca cgcgctccag
    21181 accataatgg tcggtatcaa ggatttcctg cgcctgacgc aggtcttttt tgaccttgct
    21241 gcgcgcattc cacggtacct gtaccatcca gtcgatataa ccacgcacta cggtcgcttc
    21301 tgccgacatc ggagacatca ttttcagctt ctgcagctct gcttccgctt tctcttttgc
    21361 ctctttcggc attttcgccg cgtcgatttt gcgcttcagg gcttcgtttt cgtccggcgc
    21421 gtcgtccatt tcaccgagtt ctttctgaat agctttcatt tgctcgttca gatagtactc
    21481 acgctgggat ttctccatct gctttttaac gcggttgcga atgcgtttct caacctgcag
    21541 cagatcgatt tccgattcca tcattgccat cagatattcc agacgttcgt taacgtcgga
    21601 catctccaga acggactgtt tgtcagccag tttcagcggc atatgtgcag caatggtatc
    21661 cgccagacgc gccggatcgt cgatgctatt cagcgacgtc agcacttctg gtgggatttt
    21721 tttgttcagc ttgatgtagc cttcgaactg gctgattgca gtacgcacca gcacttcctg
    21781 ttctcgctca tcaatggtcg gcgactccag atactccgcc ttcgcagaaa agtgttcgcc
    21841 attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc tcgaccagca ctttgacggt
    21901 gccgtcaggc agtttcagca tctgcaatat agaggccacg gtcccgacgg tgaaaagatc
    21961 gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg accagcataa tttttttatc
    22021 atggtccatc gccgcttcca gacaacggat agatttttcc cgcccgacaa ataaggggat
    22081 gaccatgtgc ggataaacca ccacatcgcg cagcggcaat acggggattt caatgcgttc
    22141 agaacgctca ggattcatag agctctctct tagtttaatt tccgccaggt aatcagatga
    22201 cacgactgtg cttcacgcca tctattaaca tgtacgtcag tatatgggga tgtttccccc
    22261 acattcaacg ccgagaatag aggaaaaatt aaaggggaga taaaatcccc cctttttggt
    22321 taactaattg tatgggaatg gttaattatt caccagatgc ctgttgcgct tccggcttgc
    22381 cataaatcag caacggtttg ctttgaccat caattaccga ctcgtcgata accacttttt
    22441 cgacatcttc catggacggc agatcgtaca tggtatcgag cagtgcggct tctacgatgg
    22501 aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt cttagcgata gcatccagcg
    22561 cctcgtcacg gaattccaga tccacgcctt ccagattaaa cagcgcctga tactgcttgg
    22621 tcagggcgtt tttcggctct ttgaggatct gaatcagagc ttcttcgctc agttcattca
    22681 acgttgcgac aaccggcaga cgaccaataa actcagggat aagaccaaac ttgatcagat
    22741 cttccggttc aacctgcgcc agcagctcgc cttcgcttgc tttgtcggac ttcgctttta
    22801 ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg atgggaaatc actttatcca
    22861 gaccggcaaa cgcaccgcca caaataaaca ggatcttaga ggtatcaacc tgcaagaatt
    22921 cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc agctaccgta ccttcgatca
    22981 gtttcaacag tgcctgctgt acgccttcac cggaaacgtc acgggtaata gacgggttgt
    23041 ctgacttacg agaaatcttg tcgatttcat cgatgtagac aatcccgcgc tgcgctttct
    23101 gtacgtcgta atcgcacttt tgcaacagct tctgaatgat gttttcaacg tcttcgccca
    23161 cataaccggc ttcggtcagc gtggttgcgt cggccatggt gaacgggacg tccagcaggc
    23221 gcgccagcgt ttcagccagc agcgttttac cggaaccggt cggaccgatc agcagaatgt
    23281 tacttttgcc caactcgacg ccattgctag tatcgccgtt gcgcagacgt ttgtagtggt
    23341 tgtataccgc gaccgccagc acttttttcg cctgttcctg gccgataacg taatcgtcca
    23401 ggtggttacg aatttcatgc ggcgtcggta gcgcactgcg ttcacgatgc ggtgcaactt
    23461 ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac acattcgtcg cagatataca
    23521 cggatggacc ggcaatcagc ttgcgcactt catgctggct tttgccgcaa aaagagcaat
    23581 acagcaattt gcctgagcca tctttgcgtt tatctgtcat gggtcaaaac ctcttctttg
    23641 ttctttgtgc cgcacacgac gacgcaaatg ccattctcag gcgcaagccg cttacagcgt
    23701 tgtgccgccc tggataagta tagcggcaca gttgcgcctc tggcatcaat tacgatgggt
    23761 cagaatcgaa tcgaccagac cgtattccac cgcttcaggg gcggaaagga agcgatcgcg
    23821 ctcggtatca cgttcaatct gttctaatga ttgacccgta tgaagcgcca taagttcatt
    23881 catacgccct ttaactttca gaatttcacg ggcatgaatt tcgatatcgg tcgcctggcc
    23941 ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa ttcggcaggc aaaaacgttt
    24001 accttttgcc cctgcggtca gcaagaaagc gcccatcgag gccgcctggc ccatacagat
    24061 ggtgctgaca tcaggcttga taaactgcat ggtgtcatag atagacatcc cggcagtgat
    24121 caccccgcct ggggagttaa tgtacagata gatatctttt tctgggtttt ctgcttccag
    24181 gaacagcatc tgcgccacaa tcaggttagc catgtggtct tcaacctggc cagtcagaaa
    24241 aatgacgcgt tccttaagta gacgagaata gatatcaaaa gagcgctcac cacgtgaggt
    24301 ctgttcaatg accatcggca ccagcgccat atggggtgca aagttatctc gttcgccgct
    24361 gtatgacatt tccgtctcct ggataaaatt gaaaaaacct gctgtaccga ttgtaacctt
    24421 atggacggat tatcagctag tccctgtgtt atgggtacgg catcaccata tttcaagcat
    24481 aacaatcttt tgttgttacg ctaacaccga aagaggcttt tcgcacggtt ccatgcaaaa
    24541 ttcatgacaa aaaaaagccc accacctggc ggtgacgggc ctttgtgcga atttagcgcg
    24601 ttatgctgcg taaattacgc ctgctggttc atcagctcgt tgaaagtggt ttctttttca
    24661 gtcactttcg ctttcgccag tacagcttca acagcctgtt cttccagagc aacattgcgc
    24721 atgttgtcca tcagttcttt gtttttgctg tagaactcga taacttcttt cggatcttcg
    24781 tacgcagaag ccatctcttc gatcaggcct ttcacgcgct cttcgtcagc tttcagctcg
    24841 ttggtgcgga taacttcgcc cagcagcagg ccaacaacta cgcggcgttt agcctgttct
    24901 tcgaacagtt cgcgcggcag ttccagagct tgtttttcgt tgccaccgaa acgctgtgca
    24961 gcctgacgac gcagaacgtc gatttcgctg tcgatcagcg cagccggtac gtcgatgtcg
    25021 ttagctttta ccagaccttc gatcgcctga gacttaacgc ggttacggat ggcgctcttc
    25081 agctcgcgct ccatgttttt acgcacttca gcgcgcagac cttctacgga accatcttca
    25141 acgccgaaac gtttgatgaa ttctgcagtc agttccggca gttcacgctc ttcaactttc
    25201 ttcaggttga tagcgaattt cgctgcttta cctttcaggt tttctgcgtg gtattcttcc
    25261 gggaaggtca cgtcgatggt gaactcttcg ccagctttgt ggcctttgat accgtcttca
    25321 aagcccggga tcatacgacc ctggcccatc gccagtacga aatcagacgc tttaccgcct
    25381 tcgaactctt cgccgtctac agaaccggtg aagtcgatgg ttacgcggtc ttctgcttca
    25441 acagcgccgt ctttttcttt ccaggtcgcc tgctgtttac gcagagtatc cagcatgccg
    25501 tcaacgtcag cgtcggtcac ttcaacgatc ggtttttcaa cttcgatcgc ttccagaccc
    25561 tgcagttcaa cttccggata aacttcaaac tctacagagt aggtgaagtc ttcacccagc
    25621 ttgtattcgc ccggaacata agtcggtgcg ccagccggat tgattttttc tttaatgatg
    25681 gcgtcaatga agttacggct catcaggtca cccagaacgt cctggcgtac agacgcgcca
    25741 taacgctgag caacgatatt cattggcact ttgcctttgc ggaagccgtc aatacgtact
    25801 tttttcgcaa cgttgaccag ctcgctttta acagcggtct cgatgctgtc agcagcgata
    25861 gtaatcgtta cacggcggcc aaggccttga gtggtttcaa ctgaaacttg catcttgtta
    25921 cctcaaaaaa tcacagtgct cggtcaactc ttaccccgca agcacaggtt gttggcttcg
    25981 cggaaccggg atgttcttat aatcaatcac attccctgtt accagaatca tcccgaaagc
    26041 attcaaatag gacgcggcat tatagcggca tcacttttat gagtcgagaa cggttattgc
    26101 gcgctgtcgc gtattttttc acaattcccg ggcaattttc gtctgaatgc tgggcaaaaa
    26161 gcgcaaaatc cagacaaaac aaaacggccc gcaggccgtt tttcataaaa tctttagcaa
    26221 catactggaa aagcgccgac agttgcaaat gcgtttttac gcgatgcttc ctgctccacg
    26281 acagggagga gaggcaaaga cggtgtcctg caacccttcc cactccttaa tagtgtaagt
    26341 atgcagagcc agcgcatgaa cggtagtaga gagttcctcc gctaaagtac tgtaaatcat
    26401 tcgatgacga ttcagaaaac gttcacccgt aaaacgatcg ctgaccagca caactttaaa
    26461 atggctttca gagccggctg ggacattgtg acgatagctt tcatccacta cttcgaggaa
    26521 tacgggttgg aacgccgccc ttaatttttc ttctatccgc tcacgtatca tcatgaaata
    26581 tctcctccga caacgctgag atgtcatcca tccctttaaa tactagccgc ttttaccgtt
    26641 tccattgcag cttaacaaca aatatttagg tttcgtcctg gtttttccgt caagcgtatg
    26701 aagtttactg atttaaagat tgaaacacag gcctcgcatc agcgtttctt ttaccagggg
    26761 cgattatttc gtcaacatga tgaatttaca tgccttacct acttcccctc gccgttggca
    26821 atgttatgat ggcgggaatt tttaccaaac acgcttaaga gtctctgaga gcccgaacat
    26881 gttcaaaaaa atcctcttcc cgttagttgc tctgtttatg cttgcaggat gcgcaaaacc
    26941 gccaacaact attgaagttt ccccgacgat tacactgcca cagcaggatc caagcctgat
    27001 gggcgtcacc gtaagcatta atggtgccga tcagcgtacc gatcaggcgc tggcaaaagt
    27061 cacccgcgat aatcaaatcg ttaccctgac cgcctcccgc gatctgcgtt tcctgctgca
    27121 agaagtgctg gaaaaacaga tgaccgcgcg tggttacatg gttggtccga atggcccggt
    27181 taatctgcaa atcatcgtta gccaactgta tgctgacgtg tcccagggca acgtgcgcta
    27241 caacatcgct accaaagcgg atatcgccat catcgctacc gcgcagaatg gcaacaaaat
    27301 gaccaaaaac tatcgtgcca gctacaacgt tgaaggtgcg ttccaggcct ctaacaaaaa
    27361 tatcgccgat gcggttaaca gtgtgctgac cgataccatc gctgatatgt ctcaggacac
    27421 cagcattcac gagttcatca agcagaacgc gcgttaattt ctgccctctg gcccggtgca
    27481 agccgggcct gtagacgccc atgtccagtc aatatttacg tatttttcaa cagccgcgtt
    27541 cagccatatt gctgatcctg ggttttgctt ccgggctacc gctcgccctg acatccggca
    27601 ccttacaggc ctggatgacg gtcgagaata tcgatctcaa aaccattggt ttcttctctc
    27661 tggtaggcca ggcttacgtt tttaaattcc tctggtcacc gctgatggac cgctacacgc
    27721 ccccattttt tgggcggcgg cgcggttggc tgctcgccac gcaaatcctg ttattagtcg
    27781 ccattgcggc gatgggtttt ctcgaaccag gcacccaact ccgctggatg gcggcgctgg
    27841 cggtggtgat cgctttttgc tctgcctccc aggatattgt cttcgatgcg tggaaaaccg
    27901 atgtgctccc ggcagaagaa cgtggtgcgg gcgcggcaat cagcgttctg ggttaccgtt
    27961 tagggatgct ggtttccggc ggcctagccc tgtggctggc agataaatgg ctgggctggc
    28021 agggcatgta ctggttgatg gcggcgctgt tgatcccctg tattatcgcg acgttgcttg
    28081 caccagaacc aaccgacacc attcctgtgc caaaaacgct ggaacaagcg gttgttgcac
    28141 ctctgcgaga tttctttggt cgcaataatg cctggcttat tttgcttctt atcgtgctgt
    28201 ataagctggg cgacgcattc gccatgagcc tgacaaccac gtttttgatt cgcggcgtcg
    28261 ggtttgatgc gggtgaagta ggcgtggtaa acaaaacgct tggcttacta gcgaccattg
    28321 ttggcgcatt gtacggtggg attttgatgc agcgcctgtc tctgttccgg gcactgctga
    28381 ttttcggcat tttacaaggt gcgtctaacg ctggttactg gctgctgtcg attactgata
    28441 agcatctcta cagcatgggc gcagccgtct ttttcgaaaa cctctgtggc gggatgggca
    28501 catcagcctt tgtcgcgctg ttaatgacgc tatgtaataa gtcattttcc gctactcaat
    28561 ttgccctgct ctcagcactt tctgctgtag ggcgagttta tgtcggcccc gtggcgggtt
    28621 ggtttgttga agcacacggc tggtcgacat tctatctatt ctccgtcgcc gctgccgtac
    28681 cagggcttat tttgctgctg gtttgccgcc agacgcttga atatacacga gtaaatgaca
    28741 actttatctc ccgtaccgca tatccggcag gttatgcctt tgccatgtgg acactggcgg
    28801 cgggcgtcag cctgttggcc gtgtggttac tgctgttgac gatggacgcg ctggatttga
    28861 cgcacttctc tttcctgcct gctctgctgg aagtcggggt tttagtcgcc ctttctggcg
    28921 tcgtgcttgg tggtttgctg gattatctgg cgctacgaaa aacgcatctg acgtaatctg
    28981 taaatattat ttagctgtag taatcactcg ccgtaattat tacggctaaa taaacatcag
    29041 catcgcttat taatatttgt tcttttgtgc ggcttagcgt ttggaaatta ttggcgccat
    29101 ttataaattc tatttttctt acacgattca gctaatgagt ctttattttc tcaccaccca
    29161 gttgtcactc caatgataat tatttgttaa ataattgttt tatttcgcat tggttatacc
    29221 aattgcccgt ccagactcct tctgcactcc ccttttgtta taacgccctt ttgcaacagc
    29281 ttcttaaaat caacctgata tgttttgcaa catatgtgac ctgacagcca aatccaagta
    29341 acaggaattt aatcatgttt acagtaatgt aaccttcccg taaaatgccc acacacttta
    29401 aacgccacca gatcccgtgg aattgaggtc gttaaatgag actcaggaaa tacaataaaa
    29461 gtttgggatg gttgtcatta tttgcaggca ctgtattgct cagtggctgt aattctgcgc
    29521 tgttagatcc caaaggacag attggtctgg agcaacgttc actgatactg acggcatttg
    29581 gcctgatgtt gattgtcgtt attcccgcaa tcttgatggc tgttggtttc gcctggaagt
    29641 accgtgcgag caataaagat gctaagtaca gcccgaactg gtcacactcc aataaagtgg
    29701 aagctgtggt ctggacggta cctatcttaa tcatcatctt ccttgcggta ctgacctgga
    29761 aaaccactca cgctcttgag cctagcaagc cgctggcaca cgacgagaag cccattacca
    29821 tcgaagtggt ttccatggac tggaaatggt tcttcatcta cccggaacag ggcattgcta
    29881 ccgtgaatga aatcgctttc ccggcgaaca ctccggtgta cttcaaagtg acctccaact
    29941 ccgtgatgaa ctccttcttc attccgcgtc tgggtagcca gatttatgcc atggccggta
    30001 tgcagactcg cctgcatctg atcgccaacg aacccggcac ttatgacggt atctccgcca
    30061 gctacagcgg cccgggcttc tcaggcatga agttcaaagc tattgcaaca ccggatcgcg
    30121 ccgcattcga ccagtgggtc gcaaaagcga agcagtcgcc gaacaccatg tctgacatgg
    30181 ctgcgttcga aaaactggcc gcgcctagcg aatacaacca ggtggaatat ttctccaacg
    30241 tgaaaccaga cttgtttgct gatgtgatta acaagtttat ggctcacggt aagagcatgg
    30301 acatgaccca gccagaaggt gagcacagtg cacacgaagg tatggaaggc atggacatga
    30361 gccacgcgga atccgcccat taaaggggtt gaggaagaat aaagatgttc ggaaaattat
    30421 cacttgatgc agtcccgttc catgaaccta tcgtcatggt tacgatcgct ggcattattt
    30481 tgggaggtct ggcgctcgtt ggcctgatca cttacttcgg taagtggacc tacctgtgga
    30541 aagagtggct gacctccgtc gaccataaac gcctcggtat catgtatatc atcgtggcga
    30601 ttgtgatgtt gctgcgtggt tttgctgacg ccattatgat gcgtagccag caggctcttg
    30661 cctcggcggg tgaagcgggc ttcctgccac ctcaccacta tgatcagatc ttcaccgcgc
    30721 acggcgtgat tatgatcttc ttcgtggcga tgcctttcgt tatcggtctg atgaacctgg
    30781 tggttccgct gcagatcggc gcgcgtgacg ttgcgttccc gttcctcaac aacttaagct
    30841 tctggtttac cgttgttggt gtgattctgg ttaacgtttc tctcggcgtg ggcgaatttg
    30901 cgcagaccgg ctggctggcc tatccaccgc tatcgggaat agagtacagt ccgggagtcg
    30961 gtgtcgatta ctggatatgg agtctccagc tatccggtat aggtacgacg cttaccggta
    31021 tcaacttctt cgttaccatt ctgaagatgc gcgcaccggg catgaccatg ttcaagatgc
    31081 cagtatttac ctgggcatca ctgtgcgcga acgtactgat tattgcttcc ttcccaattc
    31141 tgacggttac cgtcgcgttg ttgaccctgg atcgctatct gggcacccat ttctttacca
    31201 acgatatggg tggcaacatg atgatgtata tcaacctgat ttgggcctgg ggccacccgg
    31261 aagtttacat cctgatcctg cctgttttcg gtgtgttctc cgaaattgcg gcaaccttct
    31321 cgcgtaaacg tctgtttggt tatacctcgc tggtatgggc aaccgtctgt atcaccgtgc
    31381 tgtcgttcat cgtttggctg caccacttct ttacgatggg tgcaggcgcg aacgtaaacg
    31441 ccttctttgg tatcaccacg atgattatcg ccatcccgac cggggtgaag atcttcaact
    31501 ggctgttcac catgtatcag ggccgcatcg tgttccattc ggcgatgctg tggaccatcg
    31561 gttttatcgt caccttctcg gtgggcggta tgactggcgt gctgctggca gtaccgggcg
    31621 cggacttcgt tctgcataac agcctgttcc tgattgcgca cttccataac gttatcatcg
    31681 gcggcgtagt cttcggctgc ttcgcaggga tgacctactg gtggcctaaa gcgttcggtt
    31741 tcaaactgaa cgaaacctgg ggtaaacgcg cgttctggtt ctggatcatc ggcttcttcg
    31801 ttgcctttat gccactgtac gcgctgggct tcatgggcat gacccgtcgt ttgagccagc
    31861 agattgaccc gcagttccac accatgctga tgattgcagc cagcggtgca gtaatgattg
    31921 cgctgggtat tctctgcctc gttattcaga tgtacgtttc tattcgcgac cgcgaccaga
    31981 accgtgacct gactggcgac ccgtggggtg gccgtacgct ggagtgggca acctcttccc
    32041 cgcctccgtt ctataacttt gccgttgtgc cgcacgttca cgagcgtgat gcattctggg
    32101 aaatgaaaga gaaaggcgaa gcgtataaaa agcctgacca ctatgaagaa attcatatgc
    32161 cgaaaaacag cggtgcaggt atcgtcattg cagctttctc caccatcttc ggtttcgcca
    32221 tgatatggca tatctggtgg ctggcgattg ttggcttcgc aggcatgatc atcacctgga
    32281 tcgtgaaaag cttcgacgag gacgtggatt actacgtgcc ggtggcagaa atcgaaaaac
    32341 tggaaaacca gcatttcgat gagattacta aggcagggct gaaaaatggc aactgatact
    32401 ttgacgcacg cgactgccca cgcgcacgaa cacgggcacc acgatgcagg cggaaccaaa
    32461 atcttcggat tttggatcta cctgatgagc gactgcattc tgttctctat cttgtttgct
    32521 acctatgccg ttctggtgaa cggcaccgca ggcggcccga caggtaagga cattttcgaa
    32581 ctgccgttcg ttctggttga aactttcttg ctgttgttca gctccatcac ctacggcatg
    32641 gcggctatcg ccatgtacaa aaacaacaaa agccaggtta tctcctggct ggcgttgacc
    32701 tggttgtttg gcgccggatt tatcgggatg gaaatctatg aattccatca cctgattgtt
    32761 aacggcatgg gcccggatcg cagcggcttc ctgtcagcgt tctttgcgct ggtcggcacg
    32821 cacggtctgc acgtcacttc cggtcttatc tggatggcgg tgctgatggt acaaatcgct
    32881 cgtcgcggcc tgaccagcac taaccgtacc cgcatcatgt gcctgagcct gttctggcac
    32941 ttcctggatg tggtttggat ctgtgtgttc actgttgttt atctgatggg ggcgatgtaa
    33001 tgagtcattc taccgatcac agcggcgcgt cccatggcag cgtaaaaacc tacatgacag
    33061 gctttatcct gtcgatcatt ctgacggtga ttccgttctg gatggtgatg acaggggctg
    33121 cctctccggc cgtaattctg ggaacaatcc tggcaatggc agtagtacag gttctggtgc
    33181 atctggtgtg cttcctgcac atgaatacca aatcagatga aggctggaac atgacggcgt
    33241 ttgtcttcac cgtgctaatc atcgctatcc tggttgtagg ctccatctgg attatgtgga
    33301 acctcaacta caacatgatg atgcactaag agcggcggtt atgatgttta agcaatacct
    33361 gcaagtaacg aaaccaggca tcatctttgg caacctgatc tcggtgattg ggggattcct
    33421 gctggcctca aagggcagca ttgattatcc cctgtttatc tacacgctgg ttggggtgtc
    33481 actggttgtg gcgtcgggtt gtgtgtttaa caactacatc gacagggata tcgacagaaa
    33541 gatggaaagg acgaagaatc gggtgctggt taaaggcctg atctctcctg ctgtctcgct
    33601 ggtgtacgcc accttgctgg gtattgctgg ctttatgctg ctgtggtttg gcgcgaatcc
    33661 gctggcctgc tggctggggg tgatgggctt tgtggtttat gtcggcgttt atagcctgta
    33721 catgaaacgc cactcagtct acggcacatt gattggttcg ctctccggcg ctgcgccgcc
    33781 ggtgattggc tactgtgcag taaccggcga gttcgatagc ggcgcagcga tcctgctggc
    33841 tatcttcagc ctgtggcaga tgcctcactc ctatgccatc gccattttcc gctttaagga
    33901 ttaccaggcg gcaaacattc cggtattgcc agtggtaaaa ggcatttcgg tggcgaagaa
    33961 tcacatcacg ctgtatatca tcgcctttgc cgttgccacg ctgatgctct ctcttggcgg
    34021 ttacgctgga tataaatatc tggtggtcgc cgcggcggtg agcgtctggt ggttaggtat
    34081 ggccctgcgc ggttataaag ttgctgatga cagaatctgg gcgcgcaagc tgttcggctt
    34141 ctctatcatc gccatcactg ccctctcggt gatgatgtcc gttgatttta tggtgccgga
    34201 ctcgcatacg ctgctggctg ctgtgtggta acacaacctc tctattaaaa aggtgctacg
    34261 gcaccttttt tcttagcatt agaaacatat ccctctcgaa atatttacta aaaaatccgc
    34321 atgtttaccc cattcgtttg ccgctttaca ctagtcgcga atttaaaaca gaggtggtaa
    34381 tgaacgatta taaaatgaca ccaggtgagc ggcgcgcgac ctggggttta gggaccgtat
    34441 tctcgttgcg catgctgggc atgttcatgg ttctgccggt tctgaccacg tacggcatgg
    34501 ctctgcaagg tgccagcgaa gcattaatcg gtattgccat tggtatttat ggtctgactc
    34561 aggccgtttt tcagattccg tttggcctgc tttccgaccg cattggtcgc aaaccattaa
    34621 ttgtcggtgg gctggcagtg tttgccgccg gtagcgttat cgctgcgctt tctgactcca
    34681 tctggggaat tattctgggc cgggcgctac aaggctccgg tgcgattgcc gctgccgtta
    34741 tggcgctgct ttccgatctc acgcgcgaac aaaaccgcac caaagcaatg gcgtttatcg
    34801 gcgtgagctt tggcattacc tttgccattg cgatggtgct tggcccgatc atcactcaca
    34861 aacttgggct gcacgcgctg ttctggatga tcgctattct ggcaacaacc ggcattgcgt
    34921 tgaccatttg ggttgtaccc aacagtagca ctcacgtact taatcgtgag tccggaatgg
    34981 tgaaaggcag tttcagtaaa gtgctggcgg aaccgcggct gctaaaactc aactttggca
    35041 ttatgtgtct gcatattttg ttaatgtcga cgtttgttgc cctgccaggg caactggctg
    35101 atgcggggtt cccggcggct gaacactgga aggtctatct ggcgacaatg ctaatcgcct
    35161 ttggctcggt cgtgcctttc attatctacg ctgaagttaa gcgcaaaatg aagcaagtct
    35221 ttgtcttctg cgtcgggttg atcgtggttg cggaaattgt gttgtggaac gcgcaaacgc
    35281 agttctggca actggtggtc ggcgtgcagc ttttctttgt ggcgtttaat ttgatggaag
    35341 ccctcctgcc ctcacttatc agtaaagagt cgccagcagg ttacaaaggt acggcgatgg
    35401 gtgtttactc caccagccag tttcttggcg tggcgattgg cggttcgctg ggcggctgga
    35461 ttgacggcat gtttgacggc caaggcgtat ttctcgctgg cgcaatgctg gccgcagtgt
    35521 ggctggcagt cgccagtacc atgaaagaac cgccgtatgt cagcagctta cgcattgaaa
    35581 tcccggcgga cattgccgca aacgaggcgt taaaagtgcg tttgctggaa actgcgggtg
    35641 tcaaagaagt gttgattgca gaagaagaac attcagctta tgtgaaaatc gacagcaaag
    35701 tgacgaatcg ctttgaggtt gagcaggcaa ttcgccaggc gtaaaaaaac aggcgaaatt
    35761 gcctgctgtg ctacgtttat caggcctaca aagttcctga attacaaacc tttgtaggcc
    35821 gaaccaggcg ttcatgccgc atctgacatg aacaaagcgc aggttgtcgc gattaatcgc
    35881 ggaagttttt gaactggaac ggctgaccca gatcgccacc gcgcaccatc gccatgacag
    35941 cctgcaaatc atcacgagat ttgcccgtca cacggatctc atcgccctga atttgcgcct
    36001 gcactttcag tttgctgtct ttgatcatct tgacgatttt tttctgagtg gcgctctcaa
    36061 tgccctgttt cagtttcgct tccacaaacc aggttttacc gctatgaacg atattttccg
    36121 gtacatccag tgaactgcct tcaatgccgc gcttcagcag cttggcacgc aaaatatcca
    36181 gcaactgatt gacctggaag tcggactcgc tcaacacttt gatggttttg ctggcgtcgt
    36241 tcagctcaaa tgaggcttca acgttacgga agtcaaaacg ggactccact tcgcggctcg
    36301 cgttatcgac cgcgttacgt gcttcctgaa gatcaacttc agagacaata tcgaaagatg
    36361 gcatcttttc ctctcccttc atttttgatg cgaagcataa tacctgcaaa gttaaataac
    36421 caacagtcct gcaccaggac gtatccctta attgtgcata gtcgcaacag catgcacaat
    36481 taagggatag ccttattgaa cacccggagt ggttgcgggt gaggaggaac aatgaaaatt
    36541 accgtattgg gatgcggtgc cttagggcaa ttatggctta cagcactttg taaacagggt
    36601 cacgaagttc agggctggct gcgcgtaccg caaccttatt gtagtgtgaa tctggttgag
    36661 actgatggtt cgatatttaa cgaatcgctg accgccaacg atcccgattt tcttgccacc
    36721 agcgatctgc tcctggtgac gctgaaagca tggcaggttt ccgatgccgt caaaagcctc
    36781 gcgtccacac tgcctgtaac cacgccaata ctgttaattc acaacggcat gggcaccatc
    36841 gaagagttgc aaaacattca gcagccatta ctgatgggca ccaccaccca tgccgcccgc
    36901 cgcgacggca atgtcattat tcatgtggca aacggtatca cgcatattgg cccggcacgg
    36961 caacaggacg gtgattacag ctatctggcg gatattttgc agacagtgtt gcctgacgtc
    37021 gcctggcata acaatattcg cgccgagctg tggcgcaagc tggcagtcaa ctgtgtgatt
    37081 aatccactga ccgccatctt caattgcccg aacggtgaat tacgtcatca tccgcaagaa
    37141 attatgcaga tatgcgaaga agtcgcggcg gtgatcgaac gcgaagggca tcatacttca
    37201 gcagaagatt tgcgtgatta cgtgatgcag gtgattgatg ccacagcgga aaatatctcg
    37261 tcgatgttgc aggatatccg cgcgctgcgc cacaccgaaa tcgactatat caatggtttt
    37321 ctcttacgcc gcgcccgcgc gcatgggatt gccgtaccgg aaaacacccg cctgtttgaa
    37381 atggtaaaaa gaaaggagag tgaatatgag cgcatcggca ctggtttgcc tcgcccctgg
    37441 tagtgaagag actgaagccg tcaccacgat cgacctcatg gttcgcggcg gtatcaaagt
    37501 caccactgcc agcgtagcca gcgatggcaa cctggcaatt acctgctcgc gcggcgtgaa
    37561 gctgctggcg gatgcgccgc tggtcgaagt ggctgatggc gaatatgacg tgatcgtact
    37621 gcctggtggc attaaaggcg cggagtgttt tcgcgatagt accctgctgg ttgaaaccgt
    37681 taaacagttc caccgttccg ggcgtatcgt cgcggctatt tgcgccgcgc cagccaccgt
    37741 gctggtgccg cacgatatct ttccgattgg caatatgacc ggcttcccga cgctgaaaga
    37801 caaaattccc gctgaacaat ggcaggacaa gcgcgtcgtc tgggatgcac gggtaaaatt
    37861 gctgaccagc caggggccgg gtacagctat cgactttggt ctgaaaatta tcgacctgct
    37921 ggttgggcgc gagaaagccc atgaagtggc atcacaactg gtgatggcgg cagggattta
    37981 taattattac gagtagtgtc ggatgcggca aacgtcgcat ctgaccagat gcgacgtaaa
    38041 aaccacaaat tacgggcgat ataccttcac attgttaaag ccctgctcgc gcagatagag
    38101 cgcctgcaga cggctcatca ccccgcgctc acaccacagc agccaggttt tgttctggtc
    38161 gagatcgcca aatttggtgc tcagtttata gaacggcaga gaaaccacat caatcccttc
    38221 gactttcagt ggcttatctt cctgttcatc gacagaacgg atatcgagga tcacgtcgtt
    38281 cgggccaaag ccattgacgg tttccacttc caccacttcc tgctcggtct gctgggcgat
    38341 ttcgcggata tcaacgttat tcgcttcctc aaccacttta tcgagaatac tgaagtcgaa
    38401 cttctcttct tccgcttcaa tcttcgactt aaccgctttc accgtcgggc ttttggagat
    38461 aacaccgcaa tattccggca tcgtgcgagc aaagtcttcg gtgccaatct ggcgagccag
    38521 gttgatgatg tgctctttgt cgtaagagat cagcggacgc aggatcagcg tatcagagac
    38581 gttatcaatc aggcgcaggt tggtcagcgt ctgactggac acctggccga gcgcttcgcc
    38641 ggtgaccagc gcctgtacgc cgtaacgttc agccacttta gacgcggcac gcaccatcat
    38701 acgtttgagg ataacgccca tctgaccgtc gtcgattttc tcgagaattt ccccgacgac
    38761 cggctcgaaa ttaatagcga caaaacgcac gcggtgggag ctgccaaagc ggttccacag
    38821 ataatgtgcc acctgacgca cgccaatttc atgcgctgcg ccgccgaggt taaagaagca
    38881 gtaatgcacg cggcagccgc gacgcatcaa catataactg gaaacaccgg agtcgaaacc
    38941 accggaaatg agcgacagca catcttcctg ggtgccgatc gggaaaccgc caataccttc
    39001 gtagcggcct ttaatcagca ggagacgatc gtcttccact tccagatgga cagtcacatc
    39061 cggattggtc agcttcacgc gcgcggattc aatatgctga tttaaaccgc cgccgacgta
    39121 acgttccaca tcaatcgagc taaaatcatg tttgccacgg cgcttcacgc gtacgcagaa
    39181 ggttttgcct tccagctgat cgcgatactg aaccaacgct ttctcgaaaa tatcgtgcat
    39241 gtcggtaaac ggcacgtctt cgacttcgag aatatggtgg ataccaggaa tacgggtcag
    39301 ggcgtcgcga atagtcagac gctggttttc atctttcgcg cgaacttcga tgttatccca
    39361 gtggcggacg acagcgagcg tttcatcata gtgctttaaa acgttacgaa tgttcccggt
    39421 aaggattttt ataaagcgca agcgcacaga ttggcttttg atggtgattt ccgggaacaa
    39481 tttaatgata aacttcatgg cggcaatggt tcgttggcaa gccttaagcg acttgtatag
    39541 ggaaaaatac agcagcccac acctgcggct gcatccaagc gcggaagtat accactaaca
    39601 tcgctttgct gcgcacatca ccttaccatt gcgcgttatt tgctatttgc cctgagtccg
    39661 ttaccatgac ggggcgaaaa atattgagag tcagacattc attatgccga agaaaaatga
    39721 ggcgcccgcc agctttgaaa aggcgctgag cgagctggaa cagattgtaa cccgtctgga
    39781 aagtggcgac ctgccgctgg aagaggcgct gaacgagttc gaacgcggcg tgcagctggc
    39841 acgtcagggg caggccaaat tacaacaagc cgaacagcgc gtacaaattc tgctgtctga
    39901 caatgaagac gcctctctaa ccccttttac accggacaat gagtaatgga ctttccgcag
    39961 caactcgaag cctgcgttaa gcaggccaac caggcgctga gccgttttat cgccccactg
    40021 ccctttcaga acactcccgt ggtcgaaacc atgcagtatg gcgcattatt aggtggtaag
    40081 cgcctgcgac ctttcctggt ttatgccacc ggtcatatgt tcggcgttag cacaaacacg
    40141 ctggacgcac ccgctgccgc cgtagagtgt atccacgctt actcattaat tcatgatgat
    40201 ttaccggcga tggatgatga cgatctgcgt cgcggcttgc cgacctgcca tgtgaagttt
    40261 ggcgaagcga acgcgattct cgctggcgat gctttacaaa cgctggcgtt ctcgattcta
    40321 agcgatgccg atatgccgga agtgtcggat cgcgacagaa tttcgatgat ttctgaactg
    40381 gcgagcgcca gcggtattgc cggaatgtgc ggtggtcagg cattagattt agacgcggaa
    40441 ggcaaacacg tacctctgga cgcgcttgag cgtatacatc gtcataaaac tggcgcattg
    40501 attcgcgccg ccgttcgcct tggtgcatta agcgccggag ataaagggcg tcgtgctctg
    40561 ccagtactcg acaagtacgc agagagcatc ggccttgcct tccaggttca ggatgacatc
    40621 ctggatgtgg taggagatac tgcaacgttg ggaaaacgcc agggtgccga ccagcaactt
    40681 ggtaaaagta cctaccctgc acttctgggt cttgagcaag cccggaagaa agcccgggat
    40741 ctgatcgacg atgcccgcca gtcgctgaaa aaactggctg aacagtcact cgatacctcg
    40801 gcactggaag cgctagcgga ctacatcatc cagcgtaata aataaacaat aagtattaat
    40861 aggcccctga tgagttttga tattgccaaa tacccgaccc tggcactggt cgactccacc
    40921 caggagttac gactgttgcc gaaagagagt ttaccgaaac tctgcgacga actgcgccgc
    40981 tatttactcg acagcgtgag ccgttccagc gggcacttcg cctccgggct gggcacggtc
    41041 gaactgaccg tggcgctgca ctacgtctat aacactccgt ttgaccaatt gatttgggat
    41101 gtggggcatc aggcttatcc gcataaaatt ttgaccggac gccgcgacaa aatcggcacc
    41161 atccgtcaga aaggtggtct gcacccgttc ccgtggcgcg gcgaaagcga atatgacgta
    41221 ttaagcgtcg ggcattcatc aacctccatc agtgccggaa ttggtattgc ggttgctgcc
    41281 gagaaagaag gcaaaaatcg ccgcaccgtc tgtgtgattg gcgatggcgc gattaccgca
    41341 ggcatggcgt ttgaagcgat gaatcacgcg ggcgatatcc gtcctgatat gctggtggtc
    41401 ctcaacgaca acgaaatgtc gatttccgaa aatgtcggcg cgcttaacaa ccatctggcg
    41461 cagctgcttt ccggtaagct ttactcttca ctgcgcgaag gcgggaaaaa agttttctct
    41521 ggcgtgccac ccattaaaga gctgctcaaa cgtaccgaag aacatattaa aggcatggta
    41581 gtacctggca cgttgtttga agagctgggc tttaactaca tcggcccggt tgacggtcac
    41641 gatgtgctgg ggcttatcac cacgcttaag aacatgcgcg acctgaaagg cccgcagttc
    41701 ctgcatatca tgaccaaaaa aggtcgtggt tatgaaccgg cagaaaaaga cccaatcact
    41761 ttccacgccg tgcctaaatt tgatccctcc agcggttgtt tgccgaaaag tagcggtggt
    41821 ttgccgagct attcaaaaat ctttggcgac tggttgtgcg aaacggcagc gaaagataac
    41881 aagctgatgg cgattactcc ggcgatgcgt gaaggttccg gcatggtcga gttttcacgt
    41941 aaattcccgg atcgctactt cgatgtggca attgccgagc aacacgcggt gacctttgcc
    42001 gccggtctgg cgattggtgg gtacaaaccc attgtcgcga tttactccac tttcctgcaa
    42061 cgcgcctatg atcaggtgtt gcatgacgtg gcgattcaaa agcttccggt cctgttcgcc
    42121 atcgaccgtg cgggcattgt tggtgctgac ggtcaaaccc atcagggcgc ttttgatctc
    42181 tcttacctgc gctgcatacc ggaaatggtc attatgactc cgagcgatga aaacgaatgt
    42241 cgcctgatgc tctataccgg ctatcactat aacgatggcc cgtccgcggt gcgctacccg
    42301 cgcggcaacg cggttggcgt ggaactgacg ccgctggaaa aactaccaat tggcaaaagc
    42361 attgtgaagc gtcgtggcga gaaactggcg atccttaact ttggtacgct gatgccagaa
    42421 gcggcgaaag tcgccgaatc gctgaacgct acgctggtcg atatgcgttt tgtgaaaccg
    42481 cttgatgaag cgttaattct ggaaatggcc gccagccatg aagcgctggt caccgtagaa
    42541 gaaaacgcca ttatgggcgg cgcaggtagc ggagtgaacg aagtgctgat ggcccatcgt
    42601 aaaccagtac ccgtgctgaa cattggtctg ccggacttct ttattccgca aggaactcag
    42661 gaagaaatgc gcgccgaact cggcctcgat gccgccggta tggaagccaa aatcaaggcc
    42721 tggctggcat aatccctact ccactcctgc tatgcttaag aaattattca tggcaggagt
    42781 ggcagcatgc aatacaaccc cttaggaaaa actgaccttc gcgtttcccg actttgcctc
    42841 ggctgtatga cctttggcga gccagatcgc ggtaatcacg catggacact gccggaagag
    42901 agcagccgtc ccataattaa acgcgcgctg gaaggcggca taaatttctt tgataccgcc
    42961 aacagttatt ctgacggcag cagcgaagag atcgtcggtc gcgcactgcg ggacttcgcc
    43021 cgtcgtgaag acgtggtcgt tgcgaccaaa gtgttccatc gcgttggtga tttaccggaa
    43081 ggattatccc gtgcgcaaat tttgcgctct atcgacgaca gcctgcgacg tctcggcatg
    43141 gattatgtcg atatcctgca aattcatcgc tgggattaca acacgccgat cgaagagacg
    43201 ctggaagccc tcaacgacgt ggtaaaagcc gggaaagcgc gttatatcgg cgcgtcatcc
    43261 atgcacgctt cgcagtttgc tcaggcactg gaactacaaa aacagcacgg ctgggcgcag
    43321 tttgtcagta tgcaggatca ctacaatctg atttatcgcg aagaagagcg cgagatgctg
    43381 ccgctgtgtt atcaggaagg cgtggcggtc attccgtgga gtccgctggc gcgggggcga
    43441 ctgacgcgtc cgtggggaga aaccaccgca cggctggtgt ctgatgaggt ggggaaaaat
    43501 ctctataaag aaagcgatga aaatgacgcg cagatcgcag agcggttaac gggcgtcagt
    43561 gaagaactcg gtgcaacacg agcacaagtt gcgctggcct ggttgttgag taaaccgggc
    43621 attgccgcac cgattatcgg tacatcgcgg gaagaacagc ttgatgagct gctgaacgcg
    43681 gtggatatca cgttgaagcc ggaacagatt gctgaactgg aaacgccgta taaaccgcat
    43741 ccggtcgtag gatttaagta aatttcgctt ttcacccatt ttatctgctg aattccccat
    43801 cgtctgcgat gatggggaca aggtacgtcc catcaccttc tgtaaccata atccacctct
    43861 gcaagagaaa ttcttcgtga atgagtttgc agctattcat cgtcttcatc cctttgagcg
    43921 atttttgata gtttctccaa gtaatatccc ctaaaactgt cttatttagt caacgtaaga
    43981 ggattacaat catgatgaaa accatcacca aacaaccgat cctgtttact gatgtaccgg
    44041 ttgcagattt aagaaactca atgaagcaag atttaaatca aaatcttatt gaaaggctat
    44101 g
//
LOCUS       DAZUCE010000014        41472 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZUCE010000014 DAZUCE010000000
VERSION     DAZUCE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.014"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..100
                     /locus_tag="ACHY03_000705"
     CDS             <1..100
                     /locus_tag="ACHY03_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3903975.1"
                     /translation="LIVSGALLTEVNNNLSLSGNNLLVVNNLQNSR"
     gene            complement(132..908)
                     /gene="sseB"
                     /locus_tag="ACHY03_000706"
     CDS             complement(132..908)
                     /gene="sseB"
                     /locus_tag="ACHY03_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFS3903976.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDVEEIIQAAGSVATDTLPADEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1051..2334)
                     /gene="pepB"
                     /locus_tag="ACHY03_000707"
     CDS             complement(1051..2334)
                     /gene="pepB"
                     /locus_tag="ACHY03_000707"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFS3903977.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2393..2593)
                     /gene="iscX"
                     /locus_tag="ACHY03_000708"
     CDS             complement(2393..2593)
                     /gene="iscX"
                     /locus_tag="ACHY03_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFS3903978.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2605..2940)
                     /gene="fdx"
                     /locus_tag="ACHY03_000709"
     CDS             complement(2605..2940)
                     /gene="fdx"
                     /locus_tag="ACHY03_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFS3903979.1"
                     /translation="MPKIVILPHQELCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(2942..4792)
                     /gene="hscA"
                     /locus_tag="ACHY03_000710"
     CDS             complement(2942..4792)
                     /gene="hscA"
                     /locus_tag="ACHY03_000710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFS3903980.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALVADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(4809..5324)
                     /gene="hscB"
                     /locus_tag="ACHY03_000711"
     CDS             complement(4809..5324)
                     /gene="hscB"
                     /locus_tag="ACHY03_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFS3903981.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5420..5743)
                     /gene="iscA"
                     /locus_tag="ACHY03_000712"
     CDS             complement(5420..5743)
                     /gene="iscA"
                     /locus_tag="ACHY03_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFS3903982.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(5760..6146)
                     /gene="iscU"
                     /locus_tag="ACHY03_000713"
     CDS             complement(5760..6146)
                     /gene="iscU"
                     /locus_tag="ACHY03_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFS3903983.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6174..7388)
                     /gene="iscS"
                     /locus_tag="ACHY03_000714"
     CDS             complement(6174..7388)
                     /gene="iscS"
                     /locus_tag="ACHY03_000714"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFS3903984.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7500..7988)
                     /gene="iscR"
                     /locus_tag="ACHY03_000715"
     CDS             complement(7500..7988)
                     /gene="iscR"
                     /locus_tag="ACHY03_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFS3903985.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8258..8998)
                     /gene="trmJ"
                     /locus_tag="ACHY03_000716"
     CDS             complement(8258..8998)
                     /gene="trmJ"
                     /locus_tag="ACHY03_000716"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFS3903986.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9117..9920
                     /gene="suhB"
                     /locus_tag="ACHY03_000717"
     CDS             9117..9920
                     /gene="suhB"
                     /locus_tag="ACHY03_000717"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFS3903987.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            10065..10919
                     /gene="yfhR"
                     /locus_tag="ACHY03_000718"
     CDS             10065..10919
                     /gene="yfhR"
                     /locus_tag="ACHY03_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3903988.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     GRNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGEGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            11110..12390
                     /gene="csiE"
                     /locus_tag="ACHY03_000719"
     CDS             11110..12390
                     /gene="csiE"
                     /locus_tag="ACHY03_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFS3903989.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVCFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12382..13521)
                     /gene="hcaT"
                     /locus_tag="ACHY03_000720"
     CDS             complement(12382..13521)
                     /gene="hcaT"
                     /locus_tag="ACHY03_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFS3903990.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(13681..14571)
                     /gene="hcaR"
                     /locus_tag="ACHY03_000721"
     CDS             complement(13681..14571)
                     /gene="hcaR"
                     /locus_tag="ACHY03_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFS3903991.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            14707..16068
                     /gene="hcaE"
                     /locus_tag="ACHY03_000722"
     CDS             14707..16068
                     /gene="hcaE"
                     /locus_tag="ACHY03_000722"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFS3903992.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYTEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16065..16583
                     /gene="hcaF"
                     /locus_tag="ACHY03_000723"
     CDS             16065..16583
                     /gene="hcaF"
                     /locus_tag="ACHY03_000723"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFS3903993.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            16583..16903
                     /gene="hcaC"
                     /locus_tag="ACHY03_000724"
     CDS             16583..16903
                     /gene="hcaC"
                     /locus_tag="ACHY03_000724"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFS3903994.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVQCPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            16900..17712
                     /gene="hcaB"
                     /locus_tag="ACHY03_000725"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             16900..17712
                     /gene="hcaB"
                     /locus_tag="ACHY03_000725"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFS3903995.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            17722..18924
                     /gene="hcaD"
                     /locus_tag="ACHY03_000726"
     CDS             17722..18924
                     /gene="hcaD"
                     /locus_tag="ACHY03_000726"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFS3903996.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENKVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTTNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNHAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            19021..19443
                     /gene="yphA"
                     /locus_tag="ACHY03_000727"
     CDS             19021..19443
                     /gene="yphA"
                     /locus_tag="ACHY03_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFS3903997.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19491..20363)
                     /gene="yphB"
                     /locus_tag="ACHY03_000728"
     CDS             complement(19491..20363)
                     /gene="yphB"
                     /locus_tag="ACHY03_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFS3903998.1"
                     /translation="MTIYTLSHGALKLGVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20375..21469)
                     /gene="yphC"
                     /locus_tag="ACHY03_000729"
     CDS             complement(20375..21469)
                     /gene="yphC"
                     /locus_tag="ACHY03_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFS3903999.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAIQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(21502..22500)
                     /gene="yphD"
                     /locus_tag="ACHY03_000730"
     CDS             complement(21502..22500)
                     /gene="yphD"
                     /locus_tag="ACHY03_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3904000.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22525..24036)
                     /gene="yphE"
                     /locus_tag="ACHY03_000731"
     CDS             complement(22525..24036)
                     /gene="yphE"
                     /locus_tag="ACHY03_000731"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFS3904001.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGEIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(24059..25042)
                     /gene="yphF"
                     /locus_tag="ACHY03_000732"
                     /pseudo
     CDS             complement(24059..25042)
                     /gene="yphF"
                     /locus_tag="ACHY03_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(25139..28420)
                     /gene="yphG"
                     /locus_tag="ACHY03_000733"
     CDS             complement(25139..28420)
                     /gene="yphG"
                     /locus_tag="ACHY03_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFS3904002.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDLFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGAAPEKRLARLENNLEIALKRDDMTAELIN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICAKAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            28538..29731
                     /gene="yphH"
                     /locus_tag="ACHY03_000734"
     CDS             28538..29731
                     /gene="yphH"
                     /locus_tag="ACHY03_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFS3904003.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(29929..31182)
                     /gene="glyA"
                     /locus_tag="ACHY03_000735"
     CDS             complement(29929..31182)
                     /gene="glyA"
                     /locus_tag="ACHY03_000735"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFS3904004.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            31512..32702
                     /gene="hmpA"
                     /locus_tag="ACHY03_000736"
     CDS             31512..32702
                     /gene="hmpA"
                     /locus_tag="ACHY03_000736"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFS3904005.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(32747..33085)
                     /gene="glnB"
                     /locus_tag="ACHY03_000737"
     CDS             complement(32747..33085)
                     /gene="glnB"
                     /locus_tag="ACHY03_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFS3904006.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33146..34480)
                     /gene="glrR"
                     /locus_tag="ACHY03_000738"
     CDS             complement(33146..34480)
                     /gene="glrR"
                     /locus_tag="ACHY03_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFS3904007.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(34470..35183)
                     /gene="qseG"
                     /locus_tag="ACHY03_000739"
     CDS             complement(34470..35183)
                     /gene="qseG"
                     /locus_tag="ACHY03_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFS3904008.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(35348..36775)
                     /gene="qseE"
                     /locus_tag="ACHY03_000740"
     CDS             complement(35348..36775)
                     /gene="qseE"
                     /locus_tag="ACHY03_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFS3904009.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37351..41238)
                     /gene="purL"
                     /locus_tag="ACHY03_000741"
                     /gene_synonym="purI"
     CDS             complement(37351..41238)
                     /gene="purL"
                     /locus_tag="ACHY03_000741"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFS3904010.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
BASE COUNT         9728 a        11532 c        10482 g         9730 t
ORIGIN      
        1 acttattgtt tctggtgcgc tgttaaccga ggtaaataat aacctgagtc tctccggcaa
       61 caatttactg gtggttaaca accttcagaa cagcaggtaa gcccgaatgc cgccctttgg
      121 ggcggcatat tttagattat ccggttctgt ttaaagtcac gcaaaaaacc accccaacga
      181 cgttcataga atggcgcaat atgttcggta ataaagtggc taattccttt ttcccctttt
      241 ttcacctgac aaatatcgat tggttcatcg gcaggtaatg tatcggtcgc tacacttccc
      301 gccgcctgaa taatttcttc gacatcacca tcggcttcaa tgccaataag taaattaggc
      361 tgtgcctctt cgttctcttt aattgaacat ataaaagcac gcttcaccgg cttaatagtt
      421 ttaaataagg tggtgagtga atcaatcatt tgtgctggcg gctctgcgac ttccgataat
      481 atcagcgatt caccgccttc caggatttcc tggctgctca gcggatttcc ctcttcacca
      541 atcaacaaac tgatttcacg cggcataaat tctttaccgg tcggcagttt ggcattaagg
      601 aagagcgttt cgccaagggt catctcaaac aatgtgcgaa cgggcattac gacaaatgcc
      661 tgttcgtctt caaccgcctg ttgaagtgct tctaacgagg taaaaaaagg aatgacgctg
      721 gtgccgtctt ctttttccca gtgctgtaaa tcaagcgcgc tatcttcaac cacagcctcg
      781 ccctgcgccg ccgtaccagg cacccagacg gtggattcca gtagagtacg gaaaaaggcc
      841 gggcggtgcg ccggttcggt tgctgctttt tccagcaggt cttcaagttc attttttgtt
      901 tcggacataa gaaccacaat tcattcaacg ttcggggcac aagtgcctga tgcgccatac
      961 ttatcaggcc tacaagggat tcacaatttg ttgaatttgc aatactttgt aggccggata
     1021 aggcgtttac gccgcatccg gcatattagt ttacgccgtt aacagattag caatcgtgcg
     1081 cacaccaagt cccgtagcac ccgcagacca ctgttcaacc ggcgctttac ggtaagtcgc
     1141 cgagcagtcg atatgcagcc agccttgctg atagttctca acaaagtgcg acaggaagcc
     1201 cgctgccgtg ctcgcgcctg ccggatacgc cgcgcttccg gtattgttca gttcggcaaa
     1261 gttagacggc agctggctgc ggtggaattc cgccagcggc agacgccaga atggttcgtt
     1321 ctcttgtgag gcactcgcca gcaaacgacc ggcaagcgca tcgtcaaaac tgaacagcgc
     1381 gtgataatca ttacccagcg cagttttcgc cgccccggtg agggtcgccg catcaatgat
     1441 catttccggt ttctgcgcac tggcatcaat cagaccatcg gcaagcacca gacgcccttc
     1501 ggcatcagtg ttcatcactt caactttttt accgttgcga taggtgatga tatcgcccag
     1561 cttgaacgca ttgccgctaa tcaggttatc cgcacagcag aggaacagct tcacgcgctt
     1621 gttcagtccg cgcgtaatgg caaatgccag cgccccggta accgttgccg cgccgcccat
     1681 gtccgacttc atcgagtcca taaacgcagt ctgtttgatg ctgtagccac cggagtcaaa
     1741 agtgatacct ttacctacca ggcacgcgta cactggcgct tctttatcgc cagttgggtt
     1801 gtaatccagc gccagcaata ccggagaacg ttctgaaccg cgtccgacgg tgtgcagccc
     1861 catataacct tgctcacgca gatcttcgcc tttggtgatc cgataagtca cacgatcgcc
     1921 cgcgacgttg ctgatcagat caacagcacg ctgtgccagt tgcgatggcc ccagctcttc
     1981 cgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc atcaggcggt tatcgagttc
     2041 ctggcgctgg gcatcgtcca gatccggcca ctccacttta cgcgtgcctt tcggggcttt
     2101 gtaaccttgc cagaatgccc agcagcgatc cgcatcccag ccttcaccgc ttagctgaac
     2161 atgcttgatg cccagaccgt caatcttgcg cgccgcacgc tggatcagcc ccagatcgtc
     2221 tgccccgttc aaatgcaggg taatgccatc attattaatg ctgtaagttg ctttttctcc
     2281 ccagcgcgcg tcggcaggtt gggtagaaag ggtaattttc atcgcttctg tcattttagt
     2341 tatccttatt cgtttaacag caaaacgggc tacccccagg cagcccgttc atttactcgg
     2401 cttcgtccag ccagactaac aaaatcgctt cgaggatttt ctcgttggat gcctgcgggt
     2461 cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat atcggtgaat cgaaccgttt
     2521 tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc aatttcgcgg ctatcagtcc
     2581 acttaagtcc catactaacc tctgttaatg ctcacgcgca tggttgatag tgtaacgcgg
     2641 gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg cagcttaaac ggctttccgg
     2701 ctccagtccc caggctttgt ccagcatgtc gtcttcctgc tctgagcttt ccggcagtga
     2761 gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa gcacaggatt tttcacaggc
     2821 gtgttcaatc tcgataccgt tacgcagagc tgcgtcgaga atggtttcac cgctattagc
     2881 ttccagaaca gcgccatcag ggcagagctc ctgatgaggc aaaataacaa tctttggcat
     2941 attaaacctc gtccacggaa tggcctttca gcgcacgacg aaccgactgg tccatgcggc
     3001 gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat cgcttgttcg atggcgtcaa
     3061 cgtcatcgcc ctgcgccact tcactcaggt gagcggcagc atcgtcaatg acctgacgtt
     3121 ctgcggcgct taacagcgcg gcatcagcaa ccagcgcgcc gtgcagactt tccagcacac
     3181 gcgccgcttc tactttttgt tctgccagca ttcgggcttt tacgtcctgc tcggcatagc
     3241 tcattgagtc tttgatcatc gaagcgattt cgctatcggt cagaccgtaa gacggtttga
     3301 cctgaataga cgcctcaacg ccggtggatt tctccatcgc cgtcacgctc aaaagaccgt
     3361 cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc agccggtagc gccggaatac
     3421 cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac cagttcgcgc tcaccctgca
     3481 ttacatggat agacatcgcc gtctgaccat ctttaaaggt ggtgaaatcc tgagcgcggg
     3541 ccaccggaat agtggtatta cgcggaatca ctttctccac caggccgccc atcgtttcga
     3601 ggcccagcga cagtgggatc acatcaagca gcagcatttc gctgtctggc ttgttaccca
     3661 ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac tttatccggg tcgatggaag
     3721 tcagcggtgg acgaccgaaa aattcgccta cccgttcacg caccagcggc acgcgagtag
     3781 aaccgcccac catcaccact tccagcactt catcagcttc tacacccgcg tctttcagcg
     3841 cgcgacgaca agccagtaag gttcgtttta ccagtggcgc gatcagttca ttgaattgtt
     3901 cacggctgat ttcgccctgc cagcccgcaa cgttaacggt cacggagtcc gcatcgctca
     3961 gcgcgatttt ggctgcaatg gcggcatcca gcagttcacg ctgaacgcgg ttatcgctac
     4021 gatcaggaat gcccgcctgc tcgcgaatgt aatccgccag cagatggtcg aaatcatcgc
     4081 cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa cacgccgcga cttaagcgca
     4141 gaatggaaat atcaaacgtc ccgccaccga ggtcataaac ggcgatcacg ccttcctgac
     4201 cggaatccag cccgtaggcg atagccgcag cggtcggttc gttaagtaag cgcaggacgt
     4261 gaaggcccgc cagacgcgcc gcgtctttgg tgccctgacg ctgggcatcg tcaaagtacg
     4321 ccggaacggt gataactaca ccatccagct cgcctgccag ggcttcagtt gcccgcgccg
     4381 ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt cagcagcccc gccgccgttt
     4441 caatcatcgg caggccgttt tcgctggcct ggaattgata aggcagatgc ggatagcgtt
     4501 gctggatatc agccagcgag cgtcccatca ggcgtttaac agaactaatt gtgttggcgg
     4561 tatcgagcgc tgcattagta cgcgcgtcat aacccaccga atgcccttgc tgttgatagt
     4621 gaacaacaga tggcagcagg tgacggcctt catggtcggc taacgtttcg gcctgaccgc
     4681 tgcgcactgt cgccaccagc gagttggttg tgcccaggtc aataccggcc gccagacgac
     4741 gctgatgcgg cgcagcactc aaaccaggtt cactaatttg taataaggcc atgtttagct
     4801 tccagaaatt aaaaatcgag cagtttttct tcgagttgtt cggcactgct tcgcagttta
     4861 tcgagaaaac gcagcttacg cacggtatcc gccgccgcgt cccaggtctc gttgtctaac
     4921 tgttcaacca tcaactgatg gcgggtatca aacatctttt tcacacgttt gataaagctt
     4981 tccagccgcg cttcatcttt cgcctgttcg atctcgtcca gctcttcgcg cagctccaac
     5041 tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct cgctggcgag atcaaagccg
     5101 tgcaaagaaa gcaaatattc cgcgcgcatt aacggatgac gcagcgtttg ccaggcctgg
     5161 ttaatggttg cagattgctg tacggcggcg agttgttccg cctggcttcc gctggcgaat
     5221 ttatcaggat gatactgacg ttgtagatcc tgaaaacgca ggctcagcgc ctgggtatcg
     5281 agttgataac gggcaggcaa gccaaagagg gtgaagtaat ccataacatt ctcagggctg
     5341 cgatccgccg cgataacggc gaatcaggta aaacaaaccc cacgcgcagg cgaccacggt
     5401 ggggttatcg gtatgcgcat caaacgtgga agctttcgcc gcaaccacac tcatctttaa
     5461 cgttcgggtt ggtgaattta aacccttcgt tcaggccttc ttttacgaag tccagctgcg
     5521 taccgtccag aaattgcagg cttttgccat cgaccacgac tttcacgcct ttgtcttcaa
     5581 acacgatgtc ttccggcgtc ggttcgtcaa caaattccag tacataagcc atacctgaac
     5641 atccggaggt tctcacgccc agacgcaggc caaacccttt gccgcggtta gccagaaagg
     5701 tatttactcg cgctgctgca ctgtcgctca gtgtaatcga cataaccaaa cctcaactct
     5761 tattttgctt cacgtttgct tttatagtcc gcaatggcgg ctttgatcgc gtcttctgcc
     5821 agaatagaac agtgaatttt caccggcggc agttcaagtt cttcagcaat atcggtgttt
     5881 ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc attcggtgac cagggagctg
     5941 gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg cgtcttcaat gataccttca
     6001 tcgttgactt taatctgcaa cttcatcacg tcgccacagg ccggtgcacc caccatgccg
     6061 ctgccgacgt tctcgtcgtt gttgtcaaag gaacccacgt tacgcggatt ctcgtaatgg
     6121 tcgataactt tttcgctgta agccattata aattctcctg attccgatac cgattaatga
     6181 tgagcccatt cgatgctgtt cagatccacg ccctgcttgt acatttccca cagcggagaa
     6241 aggtcacgca gacgaccgat ggatttacga actaactcga tggtgtagtc gatctcttct
     6301 tcagtagtaa aacgacctaa agagaaacgg atagagctat gtgccagctc gtcgttcagc
     6361 cccagcgcgc gcagcacgta ggacggttcg aggcttgctg acgtacaggc ggaaccggaa
     6421 gaaaccgcga ggtctttcag cgccataatc agcgactcac cttcaacgta gttgaagctg
     6481 acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt tcaggtaaac ttcttcgata
     6541 tctttgatgc cgttccacag acggttacgc aggccgcgca gacgttccat ctcggtcgcc
     6601 atctcttctt ttgcgatgcg ataggcttcg cccattccga cgatctggtg aacaggcaga
     6661 gtgccggaac gcataccgcg ttcgtgaccg ccgccgtgca tttgcgcttc gatgcgtacg
     6721 cgcggtttac gacgcacata cagcgcaccg atacctttcg ggccatagat tttgtgaccg
     6781 gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga taggcagttt gcccacgctc
     6841 tgggttgcat caacgtgata gataatgcca cgagcacggc acatttcgcc gatagccgca
     6901 atatcctgta ccacgccgat ttcgttattc acgtgcatga tagaaacgag gatggtgtcg
     6961 tcacgcatcg ccgcttcgag ttctttcagg tcgataatac cgttacgctg cggtgccagg
     7021 taggtgactt cgaaaccttc gcgctccagc tgacggcagg tatccagtac cgctttgtgt
     7081 tcggttttgc tggtgatgat gtgcttgcct tttttctgat aaaagttggc tgcacctttg
     7141 atcgccaggt tgtcagattc ggttgcacca gaggtaaaga cgatttcacg cggatcagcg
     7201 ccgaccagat cggcaatctg attacgggcg atatctaccg cttcttcagc ctgccagccg
     7261 aaacggtgag aacgggaggc cgggttacca aaggttccgt ccatcgtcat aaactgcatc
     7321 attttctcgg caacacgcgg gtccaccggc gtggttgcgg agtagtcgag ataaatcggt
     7381 aatttcattg ctctataaac tccgtacatc actcaatgca aggaatcagg ctaccggctg
     7441 gatgtacgac cgtgtttacg gagtatttag cactccggcc tgattctgaa ttctttttat
     7501 taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg tgcgtggcgc gtcgtgagta
     7561 tgctgacgac cagacacatc cagcacttcc tggttattaa ccagttcgcc taaagtaatg
     7621 ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc acagcgcgtg ggtcaggcat
     7681 ttatcgccgc cctggcaacc gcctttaccc tgacaacggg tagcatctac agattcgtca
     7741 acggcgctaa tcacttcgcc aacggcgatg ctgctggcat ctttgcctaa cagataacca
     7801 ccgcctggtc cacgtacgct ggaaaccaga ccatttttac gcagacggga aaacagttgt
     7861 tccagataag aaagggaaat tccctgacgt tcggaaatat cagccaacgg taccgggccc
     7921 gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg catagcgccc tttagatgtc
     7981 agtctcatgt cttacttcac ctcaaactcg cccctgcccg gggtttttta ttgtaaaagt
     8041 gggggtattg catagcaggg tcaagtctga cattcccgag taaattggtc aactatttac
     8101 ttgactgatt tagtcgggta tttaaccttc agtgccattt ttttatcgtg gcgtttgtac
     8161 ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg gttctgttca ctgtaggcct
     8221 gataagacgc gttaagcatc gcatcaggca accactgtta ttcggcctta ttacctttat
     8281 tctgctgctc tatagaagcc agaatcccgc gcaggatatt caattcctgg ctttccgggc
     8341 gcgcacgggt aaacagacgg cgcaatttat tcatcacctg ccccggatgg ttttcacgga
     8401 taaaaccggt tgccagcagc gtttgttcca gatgaccata aaaacgctcc agatcatcga
     8461 ccagcggata cggcgtctct tcatgctcga cctgctcgcc attttcctga gttgccagcc
     8521 aggccatacg cacttcatag gcaataacct gaaccgccat tgccaggttc agcgaactgt
     8581 attccgggtt tgccgcaatc gcgacatgat aatggcattt ctgcaactct tcattggtca
     8641 agccgacgcg ctcgcgacca aacaccagcg ccaccggggt atttgccgct tcagcgacgc
     8701 ttttcagacc gcattcgcgc gggtcgagca tcggccacgg cagcgtgcgg gaacgtgcgc
     8761 tggtgcccac caccaggcta caaccggcta acgcttcgtc gagcgtatcg acgatgtgag
     8821 cattaccaat cacatcgctg gcccccgccg ccagggcaat agcctgggag tcaggtttca
     8881 ccagtggatt aaccagccac agattggtta atcccattgt tttcatggca cgggcaacag
     8941 aacccatatt gccggtgtgt gacgtctcca ccagcacaat tcgaatattt tgcagcattg
     9001 tctttcttcg tctaaagatt attcgcgcat cttatcataa aacgaagaca gatgccgatc
     9061 tcgctgctat actctgcgcc gttttcccgt tctttaacat ccagtgagag agaccgatgc
     9121 atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc gggtaattta attgccaaaa
     9181 actatgaaac cccggacgct gtagaagcga gccagaaagg cagtaacgat ttcgtgacca
     9241 acgtagataa agctgccgaa gcggtgatta tcgacacgat tcgtaaatct tacccacagc
     9301 acaccatcat caccgaagaa agcggtgaac ttgaaggtac tgatcaggat gttcaatggg
     9361 ttatcgatcc actggatggc actaccaact ttatcaaacg tctgccgcac ttcgcggtat
     9421 ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt ggtatacgat cctatgcgta
     9481 acgaactgtt caccgccact cgcggtcagg gcgcacagct gaacggctac cgtctgcgcg
     9541 gcagcaccgc tcgcgatctc gacggtacta ttctggcgac cggcttcccg ttcaaagcaa
     9601 aacagtacgc cactacctac atcaacatcg tcggcaaact gttcaacgaa tgtgcagact
     9661 tccgtcgtac cggttctgcg gcgctggatc tggcttacgt cgctgcgggt cgtgttgacg
     9721 gtttctttga aatcggtctg cgcccgtggg acttcgctgc aggcgagctg ctggttcgtg
     9781 aagcgggcgg catcgtcagc gacttcaccg gtggtcataa ctatatgctg accggtaaca
     9841 tcgttgctgg taacccgcgt gttgttaaag ccatgctggc gaacatgcgt gacgagttaa
     9901 gcgacgctct gaagcgttaa tgactcaggc gggtgatatc actcacccgc cctcgccttt
     9961 caggcgctat tccgaaatac ttcctcaccg ctttactttc tttcgtcact ctcccaccat
    10021 ctttccccga ttaatggatg ataaagaagt cgcaaggaaa ggttatggcg ctgccagtga
    10081 acaaacgcgt tctcaaaatt ctgtttattc tctttgttgt tgccttctgc gtttatttag
    10141 tgccgcgcgt tgccatcaac ttcttctatt atcctgacga taaaatttac ggtcccgacc
    10201 cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg tactcgtctg caaggctggt
    10261 ttatcccttc ttcgacgggc cctgctgaca acgccatcgc aaccatcatt catgctcacg
    10321 gcaatgccgg aaatatgtcc gcccactggc cgctggtcag ttggttaccc gggcgtaatt
    10381 tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc aaaaggcacg ccgtcccagg
    10441 ccggattgct ggacgatacg caaagcgcca tcaatgtggt gcgccatcgc agtgatgtaa
    10501 acccacaacg gctggtgctg ttcgggcaga gtattggcgg ggcgaatatt cttgctgtta
    10561 ttggtgaggg tgatcgtgaa ggcatacgtg cggtgatcct cgactccaca tttgcctctt
    10621 atgcaactat cgccaaccag atgatccccg gcagtggcta cttacttgat gagagttaca
    10681 gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct tttactcatt cacggtaaag
    10741 ctgatcacgt tatcccatgg cagcacagcg aaaagttgta tagcctggca aaagagccaa
    10801 aacggttgat cctaatcccg gatggcgaac acattgatgc tttttccgat cgtcacggcg
    10861 atgtttatcg cgaacagatg gtggacttta tccttagcgc gttgaatccg cagaactaac
    10921 ccatgatcgc tagcacgata atcattcaca aaaccacctt aagacatgct aatccactgg
    10981 tcagaacagt ttaagatgag aaaaattctg tgacgcttgc caacatttct gatgattagc
    11041 attcccttcg ccatttccct gagcaaactt tagctattct tatcaattat gcttatggga
    11101 gatctacaga tgatgcctac gcttgctcca ccatctgtcc tttcggctcc ccagcgccgc
    11161 tgccagatct tgctgacgct ctttcagccg gggttgaccg ccaccatggc aaccttcagc
    11221 gagcttaatg gtgtggatga tgatattgcc agtcttgata tcagcgaaac aggacgggag
    11281 atcctgcgct atcatcaact cacactgacg actggttatg acggtagcta ccgggttgaa
    11341 ggtacagtgc ttaaccaacg tttgtgttta tttcactggc tacgacgtgg tttccgtctg
    11401 tgtccgtcat ttattaccag ccaattcacc cccgccctga agagtgaact gaagcggcgc
    11461 ggaattgcgc gtaactttta cgacgatacc aatctacaag cgttagtgaa tctctgctcc
    11521 cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt tcctgtgtct gtatctgcaa
    11581 tattgtttgc tgcagcacca cgctggaatt acgcctcagt ttaatccgct ccaacgtcgc
    11641 tgggcggaat cctgccttga atttcaggta gcgcaggaaa ttggacgcca ctggcagcgt
    11701 cgggcgctcc agcctgtacc acctgatgag ccactgttta tggcactact tttttccatg
    11761 ttgcgggttc ccgatccatt gcgggatgcg catcagcggg acagacaatt gcgtcagtct
    11821 atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg tttgttttta tgatgaacag
    11881 gggttatgtg atcagcttta tacccacctc gcccaggcgt taaatcgcag tttgtttgcc
    11941 atcggtattg ataataccct gccggaagag ttcgccagac tgtatccacg cctggtacgc
    12001 accacccgcg cggcgctggc cggatttgaa agtgaatacg gcgtccatct ttctgatgag
    12061 gaaagtggtc tggtcgcggt gattttcggt gcctggctaa tgcaggaaaa cgatctgcat
    12121 gaaaaacaga ttattctact gaccgggaat gatagcgagc gagaagcgca gattgagcag
    12181 cagttacgcg aactaacgtt actgccgctc aacattaaac atatgtcggt aaaggcattt
    12241 ttgcagacag gcgctccgcg tggcgcggca ctgattattg ctccttatac catgccgtta
    12301 ccgctctttt caccaccgct gatctatacg gacctgacac tgacaacaca tcaacaggag
    12361 cagatccgca aaatgctcga atcagcatga gggaacaact ttcgggcgca aaaacattgc
    12421 cggaagcgcc accagcgcca tcacccagaa cacgccgtgg cccagatatt gatacaggaa
    12481 accggcgaaa acggtcatga tagcgatact gccgcccatc gcgacggcag agtaaaccgc
    12541 ctgtaaacgg atgacttcgc taccctggcg agcggcaata tagcgcatgg cggccaggtg
    12601 gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact atcaaccacg gcaacgccgt
    12661 agttgctccc ataatgcccc agcgcactac gccgcaaatc gccgagatca acagcatatc
    12721 gcgtgcacta caacggcgga aaagtttatt actcagtgca aagataatga cttccgccac
    12781 cacgcccagc gaccacaaat accccaccgc cgaggccgag tagccagctg cctgccagta
    12841 aatggcgcta aaaccgtaat aggccgcatg cgccccctgc aataaacaaa cgcaggccag
    12901 aaagcgccag ttctggcgaa ccagcgccaa ccacgctgac caaccggtgc tctcctgttg
    12961 gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga aagccgagca gcatggatgc
    13021 cacgcccaac gtcaacagca cgaggatcac ccgataatca aacatagtga ccagtttgcc
    13081 cgtcagcgcc gagccaatga caaacgccac cgagccccac agtcgcactt tgccgtaatc
    13141 aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg gtcaacggta ccagcggtga
    13201 gaaaaagagg ttaaagccaa tcatcaccag catcagccac gctacgtgcg cccccgccca
    13261 gaaggcgaca gcaaagagaa gtgtcagcag tgccagcacg cgcaaggcgg aaatcaggcg
    13321 agaaggatcg ctgacgcggg gcgcgatgag caaactcccg agaaaacggg caaccagacc
    13381 tgcccccaat aacaggccga tggtttctgg cgttaaacca atccctttaa gccagacgct
    13441 ccagaaaggt agaaaaatgc cgtaactaaa aaagtatgtg aaatagccga gcgccaacca
    13501 gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag gcgttagtct ggagttaatc
    13561 gaatgttggt gcaagttgaa aaataagaat attcgagcct gcttcccgat gtccgtaacg
    13621 ccagttcaca agcactcaaa aactgcgatc cgtgacgcat gttgcaaaga taatgcgtaa
    13681 ttatgccgtt acgcttgcca aacgttcctg cacaatggcg atgaaatcgc gtaatgccgg
    13741 cttcatctcc cctttcttcc acgccatcag taaagcaatg gaaggcacat ttccggcaat
    13801 gggacgaaaa acaacctgcc cggtattaaa attattcata taaccgggta tcaaagtgac
    13861 gcccagcccc atgcccacca gattcatggt caccagaata ttcgttgcca cctggacgat
    13921 atttggctgg ctgttttctt gtgcaaacca cgctttaacg atcggcgcaa gcgaaccgga
    13981 atacgccgga tcggtactga cgaaattcac gccatccagt tgcgcggcgg tgatctcttt
    14041 ttcatgtgct aaagggtgat caaccggtaa caccaccact aatggttcgt caaaaagctc
    14101 cagataatca atctccgggc tataaacagg atggcgcatt aagccgacgt cgagttcacc
    14161 acggcgaatt ttttcctcct gttgcgtggt gattaaactc accagctcaa tcaaggtgtc
    14221 tggctgtctg agacgaaaca tcggtaatac ttttggcagt aaattcactt ccgccgatgg
    14281 cacaaagcca atggttaatt gtctgtcttc ctgaacaatt ttccgcgccc gtaatttggc
    14341 attttccgct tgttcgagga ttgccagcgc gtcctggaga aaacattctc ccgccgccgt
    14401 caacgcgact ttgcgcttat ccctcaccag taacggaaca ccgacacagt tttcaagatc
    14461 gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt ttttccgccg cacgggtaaa
    14521 gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc cgtagttcca tcaccttccc
    14581 cttgttatcg aaaaaacgtc tcagctggta gaaattaact atttcacata ttagcaacca
    14641 accagcaaca tccttatggc acaaaaatag aaggtcaata catcttatct ttcaggatta
    14701 aaaaatatga ccacaccctc agatttgaac atttaccaac tgattgacac ccaaaacggt
    14761 cgcgtcactc cgcgtattta taccgacccg gacatttacc aactggagct tgagcgtatt
    14821 ttcggtcgtt gctggttatt tctcgcccac gaaagccaga tcccaaaacc cggtgatttc
    14881 tttaacacct acatgggaga agatgcagtt gtcgtggtgc gccagaaaga cggcagcatc
    14941 aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg tgagttatgc agattgcggc
    15001 aacactcgcg cctttacctg cccgtatcac ggctggtctt atggcattaa cggcgagttg
    15061 atcgatgtac cgctggaacc tcgcgcctac ccacaagggt tgtgtaaatc ccactggggg
    15121 ctaaacgaag ttccttgtgt ggagagttat aaagggctga tttttggcaa ctgggatacc
    15181 agcgcaccgg gcctgcgtga ttacctgggt gacattgcct ggtatctgga tggcatgctg
    15241 gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac aaaagtgggt gatcaactgc
    15301 aactggaaat tcccggcaga gcagttcgcc agtgaccagt atcatgctct gttcagccat
    15361 gcttctgccg ttcaggtatt aggggcgaaa gatgatggca gcgataagcg cctcggtgat
    15421 ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg cgctgcaatt tggtcaggac
    15481 ggtcacggta gcggtttctt ctttactgaa aaaccggatg ctaatgtctg ggtcgatggc
    15541 gcagtttcaa gctattaccg cgaaacctat accgaagcag aacaacgttt aggtgaagtt
    15601 cgcgccctgc gcctggcggg tcataacaat atattcccca cgctttcatg gctaaacggc
    15661 actgccacac tccgcgtctg gcatccgcgc ggccctgatc aagttgaagt gtgggcgttc
    15721 tgtattactg acaaagccgc ctccgatgaa gttaaagccg cttttgaaaa cagcgccact
    15781 cgtgcttttg gtcctgctgg ttttctcgag caggatgact cggagaactg gtgtgaaatc
    15841 cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac tgtgtctgga aatggggctt
    15901 ggtcaggaaa agcgccgcga cgacggcatt cctggcatta ctaactatat cttttcagaa
    15961 actgccgctc gtggaatgta ccaacgctgg gccgatcttc tgagtagcga aagctggcag
    16021 gaagtgctcg ataaaaccgc cgcttaccag caggaggtga tgaaatgagt gcgcaagttt
    16081 cactggagtt acatcaccgc attagccagt ttctctttca cgaagccagc ttactggacg
    16141 actggaaatt tcgtgactgg ctggcgcagc tcgacgaaga gattcgttac accatgcgca
    16201 ccacagttaa cgcgcaaaca cgcgaccgcc gcaaaggcgt ccagccaccg acaacctgga
    16261 tttttaatga caccaaagac cagctggagc ggcgaatcgc ccgtctggaa acgggcatgg
    16321 catgggcaga agagccgccg tcacgcaccc gccacttaat cagcaactgc cagataagcg
    16381 aaaccgacat cccaaacgta tttgctgttc gggtgaatta tctgctttat cgggcacaaa
    16441 aagagcgcga tgaaacgttc tatgtcggga ctcgttttga caaagttcgc cgtctggaag
    16501 atgacaactg gcgcttgctg gaacgggata tcgtcctgga tcaagcggta atcacttccc
    16561 ataacctgag tgtactgttc tgatgaatcg aatttatgcg tgtcccgttg cggatgtgcc
    16621 ggagggtgag gctctccgga tcgatacctc gcccgtcatc gccttgttca acgttggcgg
    16681 cgagttttat gccattaacg atcgttgcag ccatggtaat gcgtcgatgt cagaagggta
    16741 tctggaagat gacgccacgg tgcagtgccc gctacacgcc gccagttttt gcctgaaaac
    16801 ggggaaagcg ttatgcctgc ccgctaccga tccgctcacc acttatccag tacacgttga
    16861 aggtggtgac attttcatcg acttaccgga ggcgcagcca tgagcgatct acataacgag
    16921 tccatcttta ttaccggcgg cggatcggga ttagggctgg cgctggtcga gcgatttatc
    16981 gaagaaggcg cgcaggttgc cacgctggaa ctgtcggcgg cgaaagtcgc cagtctgcgt
    17041 cagcgatttg gtgaacatat tctggcggtg gaaggcaacg tgacctgtta tgccgattat
    17101 caacgcgcgg tcgatcagat cctgactcgt tccggcaagc tggattgttt tatcggcaat
    17161 gcgggcatct gggatcacaa tgcctcactg gttaatactc ccgcagagac gctcgaaacc
    17221 ggcttccacg agctgtttaa cgtcaacgta ctcggttacc tgctgggtgc aaaagcctgc
    17281 gctccggcgt taatcgccgg tgaaggcagc atgattttca cactatcaaa tgccgcctgg
    17341 tatcccggcg gcggtggccc gctgtacacc gccagtaaac atgccgcaac cgggcttatt
    17401 cgccaactgg cttatgaact ggctccgaaa gtacgggtga atggcgtcgg cccttgtggt
    17461 atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa gtgaaacctc aataatgcag
    17521 tctctgacgc cggagaaaat tgccgccatt ttaccgctgc aatttttccc gcaaccggct
    17581 gattttacgg gtccgtatgt gatgttggca tcgcggcgca ataatcgcgc attaagcggt
    17641 gtgatgatca acgctgatgc gggtttagca attcgcggca ttcgccacgt agcggctggg
    17701 ttggatcttt aaggaagtac gatgaaagaa aaaacgatca ttattgtcgg tggcgggcaa
    17761 gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt tcaccggtga gctgcatctg
    17821 ttttccgatg agcaacatct tccttatgaa cgccccccgc tctcgaaatc catgttgctg
    17881 gaagattccc cacagttgca gtctgtgtta cccgctcact ggtggcagga aaacaaggtt
    17941 catctgcatt ccggtgtaac catcaaaaca ttgggccgcg acacacgaga gttagtgtta
    18001 gccaacggcg aaagctggca ctgggatcag ctttttatag caaccggcgc ggcagctcga
    18061 ccgctgccgt tgcttgatgc actgggagaa cgctgcttta ccctgcgcca tgccggtgat
    18121 gccgccagac tgcgagaagt tctgcagccc gaacggtcag tcgtgattgt cggtgccgga
    18181 actattggtc tggaactggc tgccagcgcc acgcagcgca gatgtaaggt gacagtgatt
    18241 gaactggcgg caaccgtcat gggccgtaat gcaccaccgc ccgtgcaacg ctatctttta
    18301 cagcgccatc agcaggctgg tgtgcgcatt ctgctcaata atgccattga acatgtggtc
    18361 gatggtgaaa aagtagaact gacgctgcaa agtggcgaga cgcttcaggc cgatgtggtg
    18421 atttacggta ttggtatcag cgccaacgac caactggctc gcgaggccaa ccttgatact
    18481 accaatggca ttgtcattga tgaggcttgc cgcacctgcg atcccgcgat ctttgccggt
    18541 ggcgatgtgg caatcactcg tcttgataat ggtgcactac accgctgcga aagctgggaa
    18601 aacgccaata accatgcgca aattgccgct gccgcaatgt tagggctacc gcttccgcta
    18661 ctgccgccgc cgtggttctg gagtgatcag tacagtgata acttacagtt tattggcgat
    18721 atgcgtggcg atgactggct ttgtcgtggc aacccggaaa cccagaaagc gatttggttt
    18781 aatctgcaaa acggcgtgct tatcggtgcg gtaacgctga atcagggacg tgagattcgc
    18841 tcaatccgca aatggatcca gagcggcaaa acgtttgatg cgaaacagct gacagatgag
    18901 aacatcgcgc ttaaatcact gtaaccagga taattagcga atatctcaac gcctggggcg
    18961 tggcgaggta ccagagtgtg tattacgttt aaatcacatt atcttgcaaa gggattggtt
    19021 atgaactcac tacgttattt tgattttggt gctgcccgcc ccgttttgtt attaattgcc
    19081 cgtatcgccg tggtcctaat tttcattatt tttggttttc ccaaaatgat gggctttgac
    19141 ggtacggtcc aatatatggc ctcgctgggc gcaccgatgc cgatgctggc agcgattatt
    19201 gcggtagtta tggaagtgcc cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg
    19261 ctggcggtac tgtttatttt ctacacgctg ggtacggcgg tgattggtca ccattactgg
    19321 gatatgaccg gcgatgccgt cgggccaaat atgattaatt tctggaagaa tgtcagtatc
    19381 gctggcgcgt tcttgctatt ggcaattacc gggccggggg caatttctct cgatcgccgt
    19441 taggaaagat gccggatgcg gcgtgaacgc cttatccggc attttataaa ttacagccac
    19501 tctacccgca acgacatctc tgaggttgtt gattcccccg gcgcaagcgc aatgaggtca
    19561 cccccttccg ggcgatgatg gtcatccggc gcatggctca tcggctccag acagaagaaa
    19621 tcaaacgcat atcctttatc aaacgccggg tcggaaacaa agatgaaata acacggtgca
    19681 ggtggcgtcg tttctatgat gatggcataa ccctcctgcg gctgctcgat gcgggcctga
    19741 ccattccatc cggcaaaacc attgttcacc cactggcgcg gcaacggcgc tagctggtta
    19801 aaatccagtt cttgcggtag ctgctcgcaa aactcacccg ccagccactg ctcccgctcc
    19861 agccagtaac cgctcgcctg tgcctgaatc cgcgtttgcg gcgacaacgg aaaataagga
    19921 tgccagccgg taccaaatgg cagcgtctct gccccttgat tggtgacaga gagcgtcacc
    19981 gtcagcgtat ccgccgttaa atgaaacgcc tgacttaccc gatagtgata gacaccgctg
    20041 cgatgttcat acaccagaca caaactatca tcgctgtgcg agacacattg ccactcgccc
    20101 agccagccat cgccgtgtag atagtgcgca tcccactcaa cattcggttg cagttgatac
    20161 tcacgcccct gccagacaaa acggttgccg ctcacccggt ttgcaaatgg caccagcgga
    20221 aaacaagagg catcggttgc cacaccgctt tttttaccag gacgtaagag cggcgtcgta
    20281 tcgcgccaga agccttcgat tacgccgccc tggtcagaaa cgcccagctt tagcgccccg
    20341 tgggataagg tatagatggt catccgctaa ctccttaatc cgggaagtta atcacaactt
    20401 tcccgcattt gccgctcgcc atcagcgcat aggcatcacc tgcctgttcc agcgagaagc
    20461 gatgggtaat ggcgttacgc ggccacagct tccagtcagt caaatcgtgg gcgcattttt
    20521 ccatatggaa cagactggtc acccaggagc caataatccg ccgttgatgg tgcatcagat
    20581 cggcgctgac ctcgaattcc acttttccgg tttcaccaat gtaaaccacc cgcccccagt
    20641 cagcagtgga ttgcagtgcc agcaagcgac ctgcggcatt accggaacaa tcgagcgcaa
    20701 catccgcgcc accgtgggta agttcagcaa ttatctgcgg cagaccttcg gtggtcgcta
    20761 aatagccgtg atccatcacc cctaactgta ttgccatcgc cagacgttcc ggcagcatat
    20821 caacgccgat gatccgtttt gcaccgcgac ctttcgccag catcatcgcc atcatgccga
    20881 ccggccccag accgaccacc agcacgttat cgctgccgga aacttcgccg cgcaaaatcc
    20941 cttcataggc cgtgccaacg ccgcaactga taaacgcacc atcttcgtag ctcagcgcat
    21001 ccggcaggag gatcagatct ttttcttccg ccagcagata ttcggcatga ccgccgtcac
    21061 gctgccagcc gtaagccgct tttcctttgc cagtacaaga gataggaaag ccgcggcggc
    21121 agttcgggca aaaaccacag ccagaaatgt gatacaccag cacgcgatca ccctctttaa
    21181 aatggcgaca gccttgcccc atcgccacaa tttgcccgca cggctcatga ccgttgataa
    21241 agccctggta taacggttta tcgggcgccg ccgccgtggc gcggtgttga tggtagatat
    21301 agtggacatc gcttccgcaa atcccggagg atttcatttt gatcagtacc tggttaatcc
    21361 ccggcgtcgg caccgcaact tcccgcagat cgacggtcga atttcctggt aaataagctg
    21421 ccagcatcgt tttcataaat ccctcattaa caatacgatt aattttcatc cctgccgcac
    21481 ccgcgccagg ggcgttaagg tttagcgttt cgctttactg cttcgctggg tcagcaagat
    21541 attcgccagg actgccacca cgatgatgac gccgcgtacc acctgctgga aaaaggagtt
    21601 aatcccaagc aataccagac cgttaccgat taacgtaatc accagcacac caagcaacgt
    21661 accgaacaga gaaccgcgtc cgccggaaag tgccgtaccg ccgaccacga ccgcggcgat
    21721 gacgtcaaac tccagaccgt ttgccgcacc tgcgttaccg gaaccgaggc gcgccgccaa
    21781 caaaatgccg gtcaccgccg ctaataatcc cgaaagggta aagataagaa tgcgtacccg
    21841 acgaacgtta ataccgcaca actgcgccgc cgtggcatta ccgccaacgg caaaaaccga
    21901 gcgcccgaag gcggtttttc ggctaatgaa cacaaacagc gcaaacagca ccatcatgat
    21961 cagcgcggat accggcacac cgagaaattg tccgcccagc cagtccagca cctcgttttc
    22021 gtcaatcggc actggcagtg cgttggtcat aaacagcccc attccgcgca gggcgctcca
    22081 cagccccaga gtggccacga aacttggcac gttaaacacg ccacgcagca ccccggcgag
    22141 cgttcccatc agcgcgccta acagcaacac cagcagacaa gcaatcgcca acggaacttc
    22201 aaattgcagc aaaaatgcca ggcacaccga gacaaaagcc accatcggcc caacgctgac
    22261 atcaatttca ccggagataa taatcagcgt catcgcccag gcagcgatcc caatggtggc
    22321 ggcatcgcgc agcacattca tctggttatt caatgagata aagccaggcg cgttcaggga
    22381 gaagaccaga taaagaatgg caatcaccac cagcaaaccg atctcattaa tatggcgact
    22441 gacaaattgt ttgagcgaga cgctcttgcc ctgcggcaat ggtaatgacg aagcagacat
    22501 cttcaggttc ctcggtaaaa ttcatcagtg cacagacaga atggcggaca tcagctcatc
    22561 cacattgacc ggagcgtgaa actcctgcga gaacgtgccg tgctgtaata acaggatgcg
    22621 gtcacacacc agcggcaact cctccacttc actggagata aacaccacgc tttttccttc
    22681 ggcagccagc tcacggacaa tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg
    22741 cgttggctcg tcgagcaaca aaatctggct ggcggcatag acccaacgac cgatcaccac
    22801 tttttgctga ttgccgccag aaagcgtacc gatgggcgtt tcactactgg cggccttgac
    22861 cgtcatccgc tgcatcacct cttcggtcag gcggcggatg gtggaccatt gcagcacacc
    22921 gttggcgctg attttttgcc gattggtcag cactgtattt tcgtcaacgc ccaaccaggg
    22981 aatgatcccc gcttctttgc ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc
    23041 gccgtaatcg gggcgcgtga ttttctcgcc gttgataaca atttcgccct gttcatacgt
    23101 ctccagccca acaatcgcct tcagcaattc actgcgccct gcccccagca gaccagcaat
    23161 gccgagcact tcgccacgac gtagcgtaaa actgatatcc tccagcttgg gcttatggcg
    23221 taacgcacgg acttccagca cggcctgatc cacaatttcc tgaggtgcaa ccggcgcaat
    23281 atcaacgtga tcgcgcccta gcattagcga cacaatatga tgcgtggaag tgttttcgag
    23341 catcacatcg ccagccacct gcccgtcgcg cataacggtg gcacaggaag caatgcggcg
    23401 aatttcttcc atccggtggc tgacataaat caccgccacg cccagtgccg acatcttttt
    23461 caccgcgctg atcaccagtt caacttccgc actcgcaagc gaactggtag gttcatcaag
    23521 aatgaccacg cgcggctcgc ccttcatcac ccgcgcaatt tccaccagct gcttatgcgc
    23581 cgggcttagt gttgaaacaa gttgttcagg actaacgtca acgcccagcg cctgtaagca
    23641 acgttgggca tcctgcgcca tttgcaggta atcaatcatg ccgttgcggc ggggccactg
    23701 accgaggcag aggttttccg ccaccgtcag cccttccacc agacttaact cctgataaac
    23761 cgcgcgtacc cccagttcag cagcacggcg agtcagcgta gcttcgtcac cttccagtcg
    23821 cgtctcgcca atccagattt caccgctatc cggacgttca ctgccggtaa gcattcgaat
    23881 gagggtcgat ttgcccgcgc cgtttttgcc taacagcgca cgaacctcgc ctttattgag
    23941 cgtgaagtta acgttatcca acgcaacgac gccgggataa cgcttatttc ctgccaccac
    24001 ttttgctacc gggactgcct ctgttgccgt gaacataggg acctctgcga atcagcgatt
    24061 agggcagacc atcaacgtgc gttgccagcc actgtttacc gtcttccgtt ttggtataga
    24121 gatcgattgg cacctgaatc actttttcac cgtcggcttg tttattgata accttcaatg
    24181 tttacgcgaa aacagcattg cccattttct taccggaaat atccactacc gctttcagca
    24241 cctgattgtt ttccagctcc tgagcaattt cagtggtcat atccgaaccg aaaacggcaa
    24301 tttttcctgc ctgattttga ttacgtaccg cttttaccgc gccgagtgtc gcaccgcccg
    24361 attctcccat aatggcgttg agatccggcg tggagataat tagtttttca ccaacggaaa
    24421 tcgctttatc taaaacagtc ccttcctgat tagcgacaat ttgcgcgccg ggaacgcggg
    24481 cttttaatac ttcttcaaac cctttacgtc gttgcacaca aacttcaaag gcttcgcaat
    24541 tgatgacggc aattttcggc tggtcaattt tgttggcaat aaaataatcg gcggcagcgt
    24601 tacccagttt tttaccaaat tccagcgggt cacccaccag atatgccgag acatatttat
    24661 cgacaccctt ttgattaata caggtgttgt agcaaatcac cggaatatcc gcttcactgg
    24721 cgcgacgaac ggtacggcta ctgccatttt cagacactgc cgataaaata atagcatcga
    24781 cattacgcgc cacgagggta tcaacaaagg tactttcttt cgaaatatca ccctgggcgt
    24841 tagtttcaat taactgtacc tgtactgaag aatcttttgc cgcatcctga acgccctgac
    24901 gcactccagc gtaatatccc tgagtatcaa ggtatattgc gccaatggtc atttctttat
    24961 ccgcagccct ggcaaataaa gcgctaccta atagcgtagc cattaataat agattacggg
    25021 ttgttctcat ttttttaggc atagagcctc ctgtagggtt tttattaaca acggcttatt
    25081 ctaattattt tgtgatgagc ggcagcgcgt gccgctcaaa tattacagtt aatagggttt
    25141 agtgaacgta gttaaaaata aaaggcatca cagtggtgaa taatgccgct ttcggccagg
    25201 ccggattaat cgccgtcaat tcagcgcgag cagactcata ttgcgcaacc tccccgagtc
    25261 ccgcggacgc cagcatcgcc accatcaggc atttttcttt atgctgctgt tgtaaatcgc
    25321 catacagcga caacaagtca ggttgcgaga cggcaaagaa atccgcttcg atactggttt
    25381 tcgccatctc ttgcgcccac tgtttcattt cactaaaaag ttgctgtgcg atttgttgtt
    25441 cgcccagcag tcgcagcgcc attccttgcc agaagagata atcaaccggc tgatcgttgt
    25501 aataactgtg aatgttaatg gtgcgatcgc cagtcgccgc cagacgtaaa caacgcgtcg
    25561 cttcagtttc atcgccctgc gccttggcgc atatcgcctg ccagaaccag atgtcgttat
    25621 cagtttgccc cggtaaacgg ccttcgctta aattctccgg ataatgcagc gcggcatgaa
    25681 gcagttcgct ggcctgctgc ggctctctgg catcaagatg ctgccaggcg cgtaataact
    25741 ggttgaggat aaactgactg gtgaccttcc cttccccgcc ttcccacggg tggaatttgc
    25801 gcgtggcgag aatgtccgcc gctttgtctg cctgaccggt gagatgccac aaattaatta
    25861 attctgcggt catgtcgtca cgtttcaggg caatttccag attattttcc agacgcgcca
    25921 gtcgtttctc cggtgcggct ccgcttaact tatccagtaa atcccgttcg aaaagcagac
    25981 gtgcatcctg tggcgcaagc tgataagcat tatcaagata acgcgcggcc agctcataat
    26041 cgtgttgctt attccacgca tggatcgcta acccgcgcca gccgtcggca aactccggcg
    26101 acatctctac gcaacgttgc cataaggcaa tggctttgtt gtagctacgt ttgttgtagt
    26161 agaagcaggc cagtaaatgg cgagcaaacc agcactcttc aatactctcc agcgccgcca
    26221 cttcttccag cgtattcggg aaacggacaa actgcgggaa gagatcaatg gcttttgcga
    26281 ccagttcgcc acgttcggct ttcggcagca agctggcttg caggtaaagc ggcagcgtgc
    26341 gctggcagtc cagtgcgttc agcatctctg ccgccagggt gggcattccc cagttaatca
    26401 gttgcccggc ggtcatcagg gcgttaacgt cgcgtccctg acacagaccg cgccactgcg
    26461 cgagagctga ttcgctacga ccatcgaacc agttcagcca ccacagagtg gcgttcagcg
    26521 gataatcgcg cagcagtttc tcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca
    26581 ccagcagcag attatgcagg caaagcactt cctgattggt tgggcaggcg cgaagacttt
    26641 gttggcaaaa atccagcccc gcgtcgaagt taccattacg cgccgccagt cgtgccagac
    26701 cataatagcc tccggcttta ctgttgccgc tccagaccgc acgccagaaa tcctcttcgg
    26761 cttgttgata ttgtccctga cgttcgtaag cactggcgcg aatcaaactc gcctgtccgc
    26821 actgcggatt tttgttcagc gcatgtgcgc gtttcagagc ctgagtggca tacgccaccg
    26881 cttgcgggaa atctgcgcgg ttatactcca gcatcgccag cgccagatta cagcggtaat
    26941 ccagcggatc cagcgccacg ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat
    27001 gatattgctc cagatgctga ccgataaacc aggcttcatc tgtactggta atgtcttgtg
    27061 ctgccagtgg cgctttggcg acgtccggca acggcaactc ttgcggctga tgttcctgat
    27121 aactcagtac aatattgccg tcggcatcgg agagctcaat ggtcagcctt tcaggattga
    27181 taccgtgcaa cacgccctgg atggcggtcg caggcatcag tgccacggca tcatcaagta
    27241 acgcgttgca tttgccgatt tcgcggatcg ccaggcggta tccgttcaac ggagagatgg
    27301 catacagccc ccactcaatc ccccgctcac tacgctggag ttttatcacc gcatcgcggg
    27361 aggcattttg caccatgccc aaagaatgat aaggcaggaa atactgctca aaacgcttct
    27421 cttcgtaagc atcaagccag gtaaaatcgg gctggttatc ggcaaaaata ccggtcatca
    27481 gttcgatata cgggccgtta ttatcagtta ggctcttatc ccacgcctgg ccaaattcac
    27541 tgtgtcccca gctccactgc tttttacctg gcgcaatatg gtggttggca acgtgtagca
    27601 aaccgccatc ttcatcgtga caccacgcgc caacaaaatc gtactgcgat ttttcggcca
    27661 tatatgaggt tggaacgggc acatttttat agcgagaaat gtccactcca gcggagtagt
    27721 ccactttgta gtaagtgccg gtagcgatgg ggaaagcgga gactgcccgt ttgccgtgat
    27781 caaacaccgc cgttacatcc ggcgggaaga cgctctgatg cccttccccc cctttcactg
    27841 ccgggttggc ccaccacaag aaatgacgcg gtgtggcgtt gccgttatag acgcggctgg
    27901 cgatttccag cgccgcccgg tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat
    27961 gcatcggctc cgtttcgcct acccacaccg tctgtgcgcc gtcttcatgg gcttcgaggg
    28021 tgaaatcaac gggcataaag gtggtcgggc gatggtgttg cggccagtta aactcaatcc
    28081 caccggagat ccacggtccc agcagcccca ccagcgcagg tttaatgact tcattgtgat
    28141 agacaaaatc gcgctgtttc actttatccc atgcgcgatg cacccgaccg cccaattccg
    28201 gcaggatcat cactttgatg tagtcgtttt ccagccacac cgcctgccag gatttcaggg
    28261 ttttctgctc gctcagcgta tcggtcacgc cgtagggata aaccgcgccg gacgatccct
    28321 gataaacgcg attttcgagg aacatgggat gtatatcctg cggcccggtt tcataggtcg
    28381 ggatctcaac gcgctcttgc cacactttta ctggagtcat ggtgccctca atattaacaa
    28441 gacatactga attaaaagat ttgtggcagt gtattgaaca atctggcaat gttttcgcgg
    28501 aataatcacg caattaacta aacaaggttt agtgaagatg agagcctgca ttaacaatca
    28561 acagattcgc caccataaca aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc
    28621 caataaatca acgctggccc ggctggcgca aatttcgatt ccggcagtca gtaatatttt
    28681 gcaggaactg gaaagcgaaa aacgggtggt gaatatcgac gatgaaagcc agacgcgcgg
    28741 gcatagtagc ggtacatggc tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt
    28801 gacgcccacc agtattgagt gtcaggttgc taatgcttgt ttaagtccga aaggtgaatt
    28861 tgagtattta cagattgatg caccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg
    28921 ctggcatcgc caccgtaaat tgtggccgga ccgcaccatc aatctggcgc tggcaatcca
    28981 cggtcaggtt gatcctgtga ccggagtgtc gcaaaccatg ccgcaagcgc cctggacaac
    29041 gccggttgag gtgaagtatc tgctggaaga gaagctcggc attcgggtga tggtcgataa
    29101 tgactgcgtg atgctggcgc tggcggaaaa atggcaaaat aattcgcagg aacgggattt
    29161 ctgcgtgatc aacgttgatt acggcattgg ctcgtcgttc gtgattaacg agcaaattta
    29221 tcgcggcagt ttgtatggta gcggacagat tggtcacacc atcgttaatc cggatggcgt
    29281 cgtctgcgac tgtggacgtt acggctgcct ggaaaccgtc gcctcgttaa gcgcattaaa
    29341 aaaacaggcg cgggtatggc taaaatcaca accggttaat actcagcttg atcctgaaaa
    29401 actgactaca gcgcagttaa tcgctgcctg gcaaagtgga gaaccgtgga tcaccagctg
    29461 ggttgatcgc tctgccaatg ccattggttt gagtctgtat aacttcctca acatcctcaa
    29521 tattaatcag atttggttgt acggtcgcag ttgtgccttt ggtgagcact ggcttaatac
    29581 cattattcgc cagacaggat ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc
    29641 gacgcaaatt ggcttcgggc aattaagccg cgcacaacag gtgctgggga ttggctattt
    29701 gtatgttgag gcgcagttac gacagatttg atggcgcgat aacgtagaaa ggcttcccga
    29761 aggaagcctt gatgatcata aacgaaaaat tgcctgatgc gctacgctta tcaggcctac
    29821 acggagattg caatatattg aatttgcaag attttgtagg cctgagaagg tgttcatgcc
    29881 gcatccaaca tgaacaacga gcacattgtc agcaaatcac cgtttcgctt atgcgtaaac
    29941 cgggtaacgt gcgcagatgt cgagaacttt acctttgatg cgctcgataa cggcttcatc
    30001 attgatgctg tccagcacgt cacacatcca gccagccagt tctttcgctt ctgcttcttt
    30061 gaagccgcga cgcgtaattg ccggagtacc cacgcgaata ccggaggtca caaacgggct
    30121 cttcggatcg ttcggtacgc tgtttttgtt gacggtgatg ttagcacggc ccagagcggc
    30181 gtctgcttct ttaccggtca ggtttttatc gaccagatca accaggaaca gatggttatc
    30241 agtaccgccg gaaaccactt tgtagccgcg ttccaggaac acttctacca tcgctttagc
    30301 gtttttagcg acctgctgct ggtaagtttt gaactcaggc tccatcgctt ctttcagagc
    30361 aaccgcttta ccggcgatta cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc
    30421 agagttcagt tttttgtaca gctcttcgct accacctttc gccaggatca gaccgccgcg
    30481 cggacccgcc agggttttgt gagtggtggt agtaacaacg tgagcatgag gaaccgggtt
    30541 cgggtagacg ccagcagcaa ccaggcccgc aacgtgcgcc atatcaacga acaggtaagc
    30601 accgatgctg tcagcgattt cacgcatttt cgcccagtca accacgccgg aatatgcaga
    30661 gaaaccaccg ataatcattt tcggcttgtg ttctttggct tgtttttcca gatcggcgta
    30721 gtcgatatga ccggtagcat cgataccgta aggaacgatg ttgtacagtt taccggagaa
    30781 gttaaccgga gaaccgtgag tcaggtgacc gccatgcgcc aggttcatac ccagaacggt
    30841 atcacctggt tccagcagcg cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg
    30901 ctggacgtta gcgtagtcag cgccgaacag ttctttcgca cgatcgatcg ccagttgttc
    30961 aacgatatca acatactcgc aaccgccgta gtagcgtttg cccggataac cttcagcata
    31021 tttgttggtc agctgagaac cctgcgcctg cattacgcgc gggctggtgt agttttcgga
    31081 ggcgatcagt tcgatgtgct cttcctgacg tactttttcc tgctccatag cctgccacag
    31141 ttcggcatca taatcggcaa tgttcatttc acgctttaac atccgcatct cctgactcag
    31201 ctaacaataa aattttttcg cctaattttg gcggtcctga tggacaacgg cgaacagtat
    31261 aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc
    31321 aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg
    31381 tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt
    31441 acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag atgcaaaaaa
    31501 aggaaggcca tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac
    31561 tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc
    31621 cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc
    31681 tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag
    31741 cggtagaaaa aatcgcgcag aagcacacca gtttccagat caaaccggaa cagtacaaca
    31801 tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc
    31861 tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg
    31921 aaatctataa cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca
    31981 ttgtggctaa aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg
    32041 gtggcgcagt ggcagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag
    32101 gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct
    32161 atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg
    32221 ccaatgttgg cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt atggctgtcg
    32281 cagatgacac accagtgacg ttaatctctg ccggtgttgg tcaaacgcca atgctggcaa
    32341 tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag
    32401 aaaatggcga tgttcacgcc tttgccgatg aagttaagga actggggcag tcactgccgc
    32461 gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt
    32521 ttgatagcga aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa
    32581 tgcagttcta tctctgcggc ccggttggct tcatgcagtt tgccgcgaaa cagttagtgg
    32641 atctgggcgt gaagcaggaa aacattcatt acgaatgctt tggcccgcat aaggtgctgt
    32701 gatttgatgt tgccggatgg aaacatccgg caacccttga cgcggtttaa attgccgcgt
    32761 cgtcctcttc accggtacgg atgcgaatga cccgtgccac gtcaaagacg aagattttac
    32821 cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa
    32881 tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg
    32941 cgccgcggta cagctcggta tggcctttct ggcggccaaa acctttcacc tcagtgaccg
    33001 tcatgccggt aatgccgact tcagccagtg cttcgcggac atcgtccagc ttgaagggtt
    33061 taataatcgc atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt cttttgatct
    33121 gctaaacgta acacataact ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc
    33181 gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga
    33241 cgttgccttt ggtaatttgc agcagcttac gcaaatagtt gagttcaaac tgattacgtg
    33301 cctcaacaaa ggttggcagc gccgtgtttt cgccctccag cgcctgctct actaatgcat
    33361 cactaatcac cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc
    33421 gcacattacc cggccagctc gcggtcatca ggcgtttcat cgcatcggta gagaacgcgc
    33481 ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agtagcggaa
    33541 tgtcttctgt gcgttccgcc agtgccggaa ttttcaggct gacaacgttg aggcggtaat
    33601 agagatcttc acggaattcc ccgcgcgcca tcgcttttgg caggtcacgg tgagtggcag
    33661 aaataatccg cacgtcgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct
    33721 cctgcaacac gcgcagcagt ttgacctgca acggtgcggg catatcgcca atctcatcga
    33781 gaaacagcgt accgccttcc gctgcctgga ataaaccttc gcgattgctg acagcgccag
    33841 taaacgcgcc acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcac
    33901 cacagttaat agcaataaat ggtttgctgt tgcgcgggct ggcgttatgg atagcctgag
    33961 caaaaatctc tttcccggtg ccgctctggc cattaatcaa aacgctgacg tctgattgcg
    34021 ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaatcgcct
    34081 cgcgccagcg ttcgtcggtg gctggcgcgg attgttccag cgcatcatca attgcctgat
    34141 atagcgcgtc tttatcaaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag
    34201 caacggcatc gggaatagaa ccatgcgcgg taagaataat taccggcatt cccggctgca
    34261 ctttctggat ttcagcaaac agctgcatac cgtccatttc atccatccgc aggtcgctga
    34321 taactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg
    34381 tgaccacact gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat
    34441 cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg
    34501 gatggcgcgg gcttttcacc ctcgtgcggc gtatccggcg agaaatttcc agccggtttg
    34561 cgggtcgaga gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc
    34621 agctgctgtt gtagaacgtg atgttgctgg cgcaaagtat ccagctcgct gtcactggac
    34681 tgctggagtt tgctgtagcg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg
    34741 tcgcgccaga gttgatatag aggccgaacc tgtgccggga tctcggtact tagcgcatca
    34801 atgcgcgcga ccagctggcg gcgctcatac ggtgtaattt tggcgtcggc gagcaaaatc
    34861 ccttgtttaa aggtattttg ccagctgccg tcgtcatatt ggcgggcttg ctgacgcgac
    34921 tgcgcaggca ttaaacgatc agcacaatcc atcgctcgca gccagtaaag cggattggtt
    34981 tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag atagtcagcc
    35041 agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc
    35101 tgcacgcaac ccagcaatgc cagacatggc agccctgcca gccacagccg tcggggcaat
    35161 aatcgttgaa aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat
    35221 tgttgcagac agtttggaca tggacatcgc ggagaaaccg gagcgtacat gtcgtacgtg
    35281 aggatttcga gcactgcccg gggccgaact gacaaataaa atagcctgat gggataggct
    35341 gttagcatta tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc
    35401 cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc
    35461 ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg
    35521 gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa
    35581 tataaacccg cgcaccatgt aaactgctgc gaaggcaaat gttaccggat tcagccccgt
    35641 agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca
    35701 ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc
    35761 tatgagcaga aaccactgtc tccaccagcg gtgctaattc aacattctcc agttccaccg
    35821 cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc
    35881 ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa
    35941 cctgatcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat
    36001 gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta
    36061 accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac gaaacgctat
    36121 tgcccagaga acgcccttcc cccaaccggt tgatcatcct ctcgatattt ttcaccggac
    36181 caataatcat ccgcgtgaaa agcagtacca ttaccagact caccagaaat agcaccagcg
    36241 attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag
    36301 agaacactac tgtgcgcgtg gcctgtacca tttcggtatt ggcactggca aaagcttcca
    36361 gacgcgcggc agcagcagca tcgggaccac tgttgttgca ctgaagttgg gccagattgt
    36421 tcaagtcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat
    36481 cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt
    36541 ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg gtcattgctt
    36601 cgctgcgccg ggcatcgata agcgtggtgc ggttgaccag cgccgcctgg tcgctaagcg
    36661 cattcaggct ttgccaggct tgccatgcca gcaccaacag gggcagcaga atcagcaaaa
    36721 atgccagcat taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt
    36781 tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag
    36841 ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga
    36901 tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat
    36961 tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactggt
    37021 tactattatg cagtaactgt gccaataaag aaaatagttt ggtaacgtac tgattatacg
    37081 ctgctttgag gggtttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct
    37141 aaaaagagac aacttaagat aaacttatta gattacaata aaatcaataa tttaagtgtc
    37201 gccaggaagc gacacggtaa accacccatt gtcgtgattt acagacacaa aaaagctccc
    37261 tgagttggga gcttataata gcggttggta tttaatgccg gatgcggcgt gaatgcctca
    37321 tccgggctgc aaaaccaatg ggctgacgac ttaccccaac tgcttacgtg cattgcggaa
    37381 aatgcgcatc cacgggccat cctcgcccca gttttcagga tgccaggagt tgctgacagt
    37441 acggaaaaca cgttccgggt gcggcatcat aatggtgact cgaccgcttt cagtcgtgac
    37501 cgccgtaata ccgttcggag aaccgttcgg gttagccggg taggtttcag tgactttgcc
    37561 gaagttatcg acatagcgca gtgccaccag ccctttgctt tccagtgccg ccagatgcgc
    37621 cgcatcacgc acttccacgc gcccttcacc atgagagaca gcaatcggca tttgcgagcc
    37681 caccatcccc tgcaacagca gcgacgggct ttgggtcact tcaaccaggc tgaaacgcgc
    37741 ttcaaagcga tcggaggtat tgcgcacaaa acgtggccac aactcactac ctgggatcag
    37801 ttcacgcaga ttagacatca tctggcaacc gttacatacc cccagcgcca gcgtttgcgg
    37861 acggtggaag aaggttgcaa actcatcgcg tacacggtca ttgaacagga tagacttcgc
    37921 ccaaccttca ccggcaccca gcacatcacc gtaggagaaa ccaccgcacg cgaccagggc
    37981 gtggaaatcc tccaggcccg tgcgtccagc cagcaggtcg ctcatatgca cgtcgatagc
    38041 atcgaagcct gcacggtgga aagctgccgc catttcaaca tgcgagttca cgccctgctc
    38101 acgcagcaca gcaactttcg gacgtgcacc agtggcaata tacggtgctg ccacatcttc
    38161 gttgatatcg aacgacagtt ttacattcag gcccggatcg gcgtcgtttg atttcgcctg
    38221 atgctcctga tcggcacact ccgggttgtc acgcaggcgc tgcatctgcc aggtagtttc
    38281 tgcccaccag acacgcaacg tagtgcggct ttcgctgaat acagtctgcc cgttggcggt
    38341 aatcacaaaa cggtcaccgg aaaccgcctg cccaacataa tggacgcaat cagcaagccc
    38401 atgctgtgcc agtacggact cgaccgcttc acggtcagcg gcacgaacct gaatcaccgc
    38461 acccagttct tcgttaaaca acgccgccag gcgatcgtca cccagagtgg cgatatccgc
    38521 gtcaatgcca caatgaccag caaaggccat ttccgccagc gttaccagca ggccgccatc
    38581 agagcggtcg tgatacgcca gcagcttacg ctgtgcaacc agcgcctgaa tcgcgtcata
    38641 gaagcctttc agttgcgcga catcgcgtac atctgccggt ttgtcgccaa gctgacgata
    38701 aacctgcgcc agcgccgttg cgcccagcgc gttattgcct ttgcccaaat caatcagcag
    38761 cagtgcgtta tcttcggtag aaagctgcgg cgtgatggtg tgacgtacat cttccacgcg
    38821 ggcaaatgca gaaatcacca gcgacagcgg cgacgtcatt tcgcgctctt cgttaccttc
    38881 ctgccagcgg gttttcatcg acatagagtc tttacccacc gggatcgtca ggcccagcgc
    38941 cggacaaagc tcttcgccca cggctttaac ggcttcatac aggcccgcat cttcaccagg
    39001 gtggcctgcc gccgccatcc agttggcgga aagtttaatg cgtttgatat cgccaatctg
    39061 tgttgcggca atattggtta acgcttcacc gaccgccaga cgggcagagg cggcgaaatc
    39121 cagcagcgcc accggcgcac gctcgccaat cgccatcgct tcaccgtagt agctgtcgag
    39181 gctggcggta gtgaccgcgc agttagcgac cggcacctgc cacggcccca ccatctgatc
    39241 gcgcgccacc atgccggtta cgctgcggtc accgatggtc accaggaaag ttttctccgc
    39301 tacggtcggc agatgcagta cgcgtttcac cgcgtctgca atggtgatcc cttcacggac
    39361 cagcgcatcg cccttcgctt tcagcgtttg tacatcgcgg gtcatcttcg gcgttttacc
    39421 aagcaggacg tccagcggca gatcgatcgg ctgattatca aaatgacgat cgtgcagaga
    39481 aagatgcagt tcttcggtcg cttcaccaat caccgcgtag ggtgcgcgct cacgcttaca
    39541 caattcgtca aacagcggta attgatcggc agcaaccgcc agcacgtagc gttcctggga
    39601 ttcgttacac cagatttcca gcgggctcat gcccggttcg tcgcttagaa tatcgcgcag
    39661 ttcaaattta ccgccgcgcc cgccgtcgct caccagttcc ggcatcgcgt tagaaagacc
    39721 gccagcgccg acgtcgtgga taaacaggat tgggttggca tcaccaagct gccagcaacg
    39781 gtcgatcact tcctggcagc gacgctccat ctccgggttg tcgcgctgta cggaagcaaa
    39841 gtcgaggtcg gcatcagact gacctgacgc catagaagac gctgcaccac caccaagacc
    39901 gatgttcatt gccgggccgc cgagaacgac cagcttcgca ccgacgttga tctcgccttt
    39961 ttgtacgtga tcggcgcgaa tgttgccgat cccgcccgcc agcatgatcg gtttgtgata
    40021 accgcgcagc tcttcgccgt tgtggctgtt cactttttct tcataagtac ggaagtagcc
    40081 attcagtgcc ggacgaccaa attcgttgtt aaatgccgcg ccgcccagcg ggccttcggt
    40141 catgatgtcc agcgcggtga caatgcgctc aggcttaccg aaatcttctt cccacggctg
    40201 ttcgaagcca ggaattcgca ggttggaaac ggagaaacca accaaacccg ctttcggctt
    40261 cgcgccgcgc ccggtggcac cttcatcgcg gatttcaccg ccagaaccgg tcgccgcgcc
    40321 cggccacgga gaaatcgccg tcgggtggtt gtgagtttcg actttcatca gaatatgcgc
    40381 cggttcctga tggaaatcgt agcggcccgt ttcgtggtca gcaaagtagc ggcccacttc
    40441 agaaccttcc attacggcgg cgttatcttt ataagcagag agaacgtgat ctggcgtggt
    40501 ttcgaaagta tttttgatca tcttgaacag cgatttcggc tgctgttcac cgtcgataat
    40561 ccagtcggca ttaaaaattt tgtggcggca gtgctcagag ttcgcctgag cgaacatata
    40621 cagctcgatg tcgttcgggt tacgaccaag ctttgtgaaa gcatcctgca gatagtcaat
    40681 ttcatcttcc gccagagcca ggccaagacg caggttagcg tcgatcagcg cctgacggcc
    40741 ctgccccagc aaatcaacgc tggtaaccgg agtcggttga tggtgggcaa acaactgctc
    40801 tgcatcatct aaagcaaaaa agaccgtttc catcatgcgg tcgtgcagtt cagcggtaac
    40861 ctgctgccat tgttcattgg tcagcgtacc ggcttctata tagtaagcaa cgccgcgctc
    40921 aaggcggttt acctgttgta gcccgcagtt atgggcaata tcggtcgctt tcgaagacca
    40981 gggagagatg gtgccaggac gcggggtcac cagcaggagt ttgccttgcg gggcgtggct
    41041 ggcgagtgcc gggccatatt tcagcaggcg ttcaagttgt gcgtgctcat catcgtttaa
    41101 cggcgcattg aggtcagcaa aatggacata ctcggcgtaa atattgtgaa ccgggagcct
    41161 ggcagcctga aaacgtgcca gcagtttgtt gattcggaat gccgacagtg caggcgaacc
    41221 acgcagaatt tccatcataa gtctctcgtc ttctaagctt tcggtgtacc caaggggggg
    41281 aaacgggcgt cattataaag aatctgatgc gctgacgaaa ccgtttgcgt ggaaataaaa
    41341 tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt aataagttgc catctggcgc
    41401 aggtttacgc aaaatgccgc tcatttatga gtaaaccttt cactattatt acgttttttc
    41461 aagctgggac gc
//
LOCUS       DAZUCE010000015        40805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZUCE010000015 DAZUCE010000000
VERSION     DAZUCE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.015"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            175..297
                     /locus_tag="ACHY03_000742"
     CDS             175..297
                     /locus_tag="ACHY03_000742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904011.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
     gene            294..860
                     /gene="mntP"
                     /locus_tag="ACHY03_000743"
     CDS             294..860
                     /gene="mntP"
                     /locus_tag="ACHY03_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFS3904012.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(857..1666)
                     /gene="rlmA"
                     /locus_tag="ACHY03_000744"
     CDS             complement(857..1666)
                     /gene="rlmA"
                     /locus_tag="ACHY03_000744"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFS3904013.1"
                     /translation="MSFSCPLCHQPLTREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLNEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1832..2041)
                     /gene="cspE"
                     /locus_tag="ACHY03_000745"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1832..2041)
                     /gene="cspE"
                     /locus_tag="ACHY03_000745"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFS3904014.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2054..2197)
                     /gene="yobF"
                     /locus_tag="ACHY03_000746"
     CDS             complement(2054..2197)
                     /gene="yobF"
                     /locus_tag="ACHY03_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFS3904015.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2868..3155)
                     /gene="yebO"
                     /locus_tag="ACHY03_000747"
     CDS             complement(2868..3155)
                     /gene="yebO"
                     /locus_tag="ACHY03_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFS3904016.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
     gene            complement(3230..3373)
                     /gene="mgrB"
                     /locus_tag="ACHY03_000748"
     CDS             complement(3230..3373)
                     /gene="mgrB"
                     /locus_tag="ACHY03_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFS3904017.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3532..3771
                     /gene="yobH"
                     /locus_tag="ACHY03_000749"
     CDS             3532..3771
                     /gene="yobH"
                     /locus_tag="ACHY03_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFS3904018.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(3915..4706)
                     /gene="kdgR"
                     /locus_tag="ACHY03_000750"
     CDS             complement(3915..4706)
                     /gene="kdgR"
                     /locus_tag="ACHY03_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFS3904019.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4883..6256
                     /gene="yebQ"
                     /locus_tag="ACHY03_000751"
     CDS             4883..6256
                     /gene="yebQ"
                     /locus_tag="ACHY03_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3904020.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(6305..7186)
                     /gene="htpX"
                     /locus_tag="ACHY03_000752"
     CDS             complement(6305..7186)
                     /gene="htpX"
                     /locus_tag="ACHY03_000752"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFS3904021.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7378..9426)
                     /gene="prc"
                     /locus_tag="ACHY03_000753"
     CDS             complement(7378..9426)
                     /gene="prc"
                     /locus_tag="ACHY03_000753"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFS3904022.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9446..10144)
                     /gene="proQ"
                     /locus_tag="ACHY03_000754"
     CDS             complement(9446..10144)
                     /gene="proQ"
                     /locus_tag="ACHY03_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFS3904023.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10241..10738)
                     /gene="msrC"
                     /locus_tag="ACHY03_000755"
     CDS             complement(10241..10738)
                     /gene="msrC"
                     /locus_tag="ACHY03_000755"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFS3904024.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10868..12151
                     /gene="yebS"
                     /locus_tag="ACHY03_000756"
     CDS             10868..12151
                     /gene="yebS"
                     /locus_tag="ACHY03_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFS3904025.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12120..14753
                     /gene="yebT"
                     /locus_tag="ACHY03_000757"
     CDS             12120..14753
                     /gene="yebT"
                     /locus_tag="ACHY03_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFS3904026.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSFSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVINRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            14833..16272
                     /gene="rsmF"
                     /locus_tag="ACHY03_000758"
     CDS             14833..16272
                     /gene="rsmF"
                     /locus_tag="ACHY03_000758"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFS3904027.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVDNFPFSPVKD
                     REAAQIRQAAAGVGLNWDENLRLWQRDKEVWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNENAFELTPQEAEEWYRGRDVYPQAEPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            16390..16626
                     /gene="yebV"
                     /locus_tag="ACHY03_000759"
     CDS             16390..16626
                     /gene="yebV"
                     /locus_tag="ACHY03_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFS3904028.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16731..16922
                     /gene="yebW"
                     /locus_tag="ACHY03_000760"
     CDS             16731..16922
                     /gene="yebW"
                     /locus_tag="ACHY03_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFS3904029.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRSAQEYGDF"
     gene            complement(16923..17579)
                     /gene="pphA"
                     /locus_tag="ACHY03_000761"
     CDS             complement(16923..17579)
                     /gene="pphA"
                     /locus_tag="ACHY03_000761"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFS3904030.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(17975..18316)
                     /gene="yebY"
                     /locus_tag="ACHY03_000762"
     CDS             complement(17975..18316)
                     /gene="yebY"
                     /locus_tag="ACHY03_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFS3904031.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDVAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(18328..19200)
                     /gene="copD"
                     /locus_tag="ACHY03_000763"
     CDS             complement(18328..19200)
                     /gene="copD"
                     /locus_tag="ACHY03_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFS3904032.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLSPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(19204..19578)
                     /gene="yobA"
                     /locus_tag="ACHY03_000764"
     CDS             complement(19204..19578)
                     /gene="yobA"
                     /locus_tag="ACHY03_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFS3904033.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            19717..19947
                     /gene="holE"
                     /locus_tag="ACHY03_000765"
     CDS             19717..19947
                     /gene="holE"
                     /locus_tag="ACHY03_000765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFS3904034.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            20049..20705
                     /gene="yobB"
                     /locus_tag="ACHY03_000766"
     CDS             20049..20705
                     /gene="yobB"
                     /locus_tag="ACHY03_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFS3904035.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            20729..21391
                     /gene="exoX"
                     /locus_tag="ACHY03_000767"
     CDS             20729..21391
                     /gene="exoX"
                     /locus_tag="ACHY03_000767"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFS3904036.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(21388..23448)
                     /gene="ptrB"
                     /locus_tag="ACHY03_000768"
     CDS             complement(21388..23448)
                     /gene="ptrB"
                     /locus_tag="ACHY03_000768"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFS3904037.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATSSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(23657..24316)
                     /gene="yebE"
                     /locus_tag="ACHY03_000769"
     CDS             complement(23657..24316)
                     /gene="yebE"
                     /locus_tag="ACHY03_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFS3904038.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERATIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(24642..24998)
                     /gene="yebF"
                     /locus_tag="ACHY03_000770"
     CDS             complement(24642..24998)
                     /gene="yebF"
                     /locus_tag="ACHY03_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFS3904039.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(25065..25355)
                     /gene="yebG"
                     /locus_tag="ACHY03_000771"
     CDS             complement(25065..25355)
                     /gene="yebG"
                     /locus_tag="ACHY03_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFS3904040.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            25489..26667
                     /gene="purT"
                     /locus_tag="ACHY03_000772"
     CDS             25489..26667
                     /gene="purT"
                     /locus_tag="ACHY03_000772"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFS3904041.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(26723..27364)
                     /gene="kdgA"
                     /locus_tag="ACHY03_000773"
                     /gene_synonym="eda"
     CDS             complement(26723..27364)
                     /gene="kdgA"
                     /locus_tag="ACHY03_000773"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFS3904042.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(27401..29212)
                     /gene="edd"
                     /locus_tag="ACHY03_000774"
     CDS             complement(27401..29212)
                     /gene="edd"
                     /locus_tag="ACHY03_000774"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFS3904043.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(29447..30922)
                     /gene="zwf"
                     /locus_tag="ACHY03_000775"
     CDS             complement(29447..30922)
                     /gene="zwf"
                     /locus_tag="ACHY03_000775"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFS3904044.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            31260..32129
                     /gene="hexR"
                     /locus_tag="ACHY03_000776"
                     /gene_synonym="yebK"
     CDS             31260..32129
                     /gene="hexR"
                     /locus_tag="ACHY03_000776"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFS3904045.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            32257..33699
                     /gene="pyk"
                     /locus_tag="ACHY03_000777"
     CDS             32257..33699
                     /gene="pyk"
                     /locus_tag="ACHY03_000777"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFS3904046.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(33830..34801)
                     /gene="lpxM"
                     /locus_tag="ACHY03_000778"
                     /gene_synonym="msbB"
     CDS             complement(33830..34801)
                     /gene="lpxM"
                     /locus_tag="ACHY03_000778"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFS3904047.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(34921..36243)
                     /gene="mepM"
                     /locus_tag="ACHY03_000779"
     CDS             complement(34921..36243)
                     /gene="mepM"
                     /locus_tag="ACHY03_000779"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFS3904048.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(36259..37191)
                     /gene="znuA"
                     /locus_tag="ACHY03_000780"
     CDS             complement(36259..37191)
                     /gene="znuA"
                     /locus_tag="ACHY03_000780"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFS3904049.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            37270..38025
                     /gene="znuC"
                     /locus_tag="ACHY03_000781"
     CDS             37270..38025
                     /gene="znuC"
                     /locus_tag="ACHY03_000781"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFS3904050.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            38022..38807
                     /gene="znuB"
                     /locus_tag="ACHY03_000782"
     CDS             38022..38807
                     /gene="znuB"
                     /locus_tag="ACHY03_000782"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFS3904051.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(38951..39961)
                     /gene="ruvB"
                     /locus_tag="ACHY03_000783"
     CDS             complement(38951..39961)
                     /gene="ruvB"
                     /locus_tag="ACHY03_000783"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFS3904052.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(39970..40581)
                     /gene="ruvA"
                     /locus_tag="ACHY03_000784"
     CDS             complement(39970..40581)
                     /gene="ruvA"
                     /locus_tag="ACHY03_000784"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFS3904053.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(40720..40785)
                     /gene="yobI"
                     /locus_tag="ACHY03_000785"
     CDS             complement(40720..40785)
                     /gene="yobI"
                     /locus_tag="ACHY03_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFS3904054.1"
                     /translation="MYIFITHFFTEYVILKYLLPI"
BASE COUNT         9877 a        10453 c         9993 g        10482 t
ORIGIN      
        1 acaaggcaat aaaataatat attccaacag ttgttttata ttctcaaaat atgttaaggt
       61 tgcgccctca tttggggagt agccaatttc cagattccgg aaatgtacgt gtcaacatac
      121 tcgttgcaaa acgtggcacg tacggactga agactttcag tcaggcgaga ccatatgcac
      181 atcaatcgct atgcctgcat gaacgcgaaa acccatcgcg gcttgttgtt catgtatgca
      241 tgtttgctgg gggcgatgat gtgttttatg gataccccgg tcaggacgtt gtcatgaata
      301 tcactgctac tgttcttctt gcgtttggta tgtcgatgga tgcatttgct gcatcaatcg
      361 gtaaaggtgc caccctccat aaaccgaaat tttctgaagc attgcgaacc ggccttattt
      421 ttggtgccgt cgaaaccctg acgccgctga tcggctgggg aatgggcatg ttagccagcc
      481 ggtttgtcct tgaatggaat cactggattg cgtttgtgct gctgatattc ctcggcgggc
      541 gaatgattat tgagggtttt cgtggcgcag atgatgaaga tgaagagccg cgccgtcggc
      601 acggtttctg gctactggta accaccgcga ttgccaccag cctggatgcc atggctgtgg
      661 gtgttggtct tgctttcctt caggtcaaca ttatcgcgac cgcattggcc attggttgtg
      721 caaccttgat tatgtcgaca ttagggatga tggttggtcg ctttatcggc tcaattattg
      781 ggaaaaaagc ggaaattctc ggcgggctgg tgctgatcgg catcggcgtc cagatcctct
      841 ggacgcactt ccacggttaa taagaacgct gccagaggtg aatattaaag tccgtctggc
      901 agtcgaacac ttcttttgct gccagtgttt gccagacttc tggcttcgca cgccaggcaa
      961 acggcgtcat ctgcaataat gcgacggctt catcaccgcg aagacgcatc ggataacaca
     1021 actcatcact ctgctgtaat gtaaaacctt ccagttgttc tgcatgaggt gcatgaagat
     1081 gtacttcatt gtaaatcagc cccttcagct ccatcaaatg tcgcggtccc ggcgtggcag
     1141 taatgaccca gccgccgggc ttcactactc gtgctaattc ttctgcttta cacggcgcgt
     1201 aaatacgtat tatggcgtcc atactggtat cggaaaacgg caaacggtgg ctggaagcga
     1261 cacaaagagt gacctgcgga tagcgtttcg ccgccgcttt tatcgctacc ttcgaaacat
     1321 ccagaccaaa ggtggtgatt tcgggcaacg catcggcaaa tgcgtgtgtg taataccctt
     1381 caccacagcc aatatccagc accgccgtgg ccttttcatt aagccgttcc cgcagttggg
     1441 cgacaattgc atcacgcagc ggctgataat gtccggcatc taagaatgcg cggcgtgctt
     1501 gcatcatttc cgcgctgtcg cccggatcac gagaccgttt atgctgaacg ggcagcagat
     1561 tgacataccc ttctttcgcc atatcaaact gatgtcgttg gggacagata tagctgtttt
     1621 tttcacgcgt aagaggctga tggcaaagtg gacaggaaaa cgacatgaca actccggcag
     1681 gatactaaag gccgcaagtg taacgcgaat cacgcggcgg gcaaatatcc gaacgccagg
     1741 agcgaagata aagcatagta aaaagcctcg cattcgacga ggctttatag cgagattgaa
     1801 gcgtattcac acttcagatc agtggattcg atcagatagc tgttacgtta acagctgccg
     1861 gacctttctg gccgtcctga atttcgaact caacgttctg accttcagcc agagttttga
     1921 agccattacc ctggatagcg gagaagtgta cgaacacgtc tttgctgcca tcagcaggag
     1981 taatgaagcc aaaaccttta gactcgttga accacttaac ctgaccttta atctttgcca
     2041 tttgaaaaat tccttagatt gttttcttcg cccgcaggca taacatagat aaaactgaca
     2101 cattactgca tgaggcacca atataaggct cggcagagaa gcggtattca acgtcaacgt
     2161 gtttactcag gacttcttta ctgaaaatgc cacacataaa cagaactgta cctcgtttaa
     2221 cccgaaatct gttatcacat acaacgttaa ttatggcaag ccatttttaa acatgtctcg
     2281 atcagacgca caaatctcga tacgtcttcc actttttttg cacacttatg caacggaata
     2341 cgcgccgata tatcattgtg cttaaccttg ccagttcagg cagatactta aactggcgta
     2401 gtttctaaca tagttcaata agttcagttg ttccaggcca gtgggagaag ttattacata
     2461 gtgcgtgcaa tatcacattt tctgctatgc aatgaataaa aagttatatc actttttctc
     2521 ataaaacagt cagttaacgg ctatcaatta ccctaaagag aagtcaatcc ccaaagggat
     2581 tgtaaattta aaataagaaa aattgatgag tgagcaaaaa aaatcaagag agaaacgttt
     2641 cgctactaaa taataccacg cttaatcaca aatccgatgt aaacatccta ataaattaat
     2701 gaggaaaata tctttttctg atatcccgtt acgcaaataa ataatatgat aattatttga
     2761 agtcagacca gagttttaat ttgaagcagt tgcaccatga cagtgcgtat aatcaggatg
     2821 aattgagtaa cccttcaacg acggggttac tcaagattga ggaaggatta tcgttcggcg
     2881 accaggcgaa taatatcttc gtcttcaacc gatttttgac tctcaatggt ctttttatct
     2941 gctttctctg gttcgtttgc ttcgcacaaa cgacgtaaca gtgcattttg acgtttttgc
     3001 tgatccagca acgcctcaag cagttcaatc tgttcgttag tccgagaact ggcacgatta
     3061 ataaaaaacc acaagatgag cccgataaga agaaccacca ccgatacaac caaagacgca
     3121 atgttcatca cgcctgaatt tacaacttcg ttcatttcac cacctctgag tagaggcgct
     3181 attctaccac tgctggagag gaagaaaatc tagtgctgaa aaaatgatat caccacggga
     3241 taaactggtt aatggcacaa attccgctga aaaattgtac atcctgatcg cacatcatgt
     3301 tgaatacctg cgcccaaagc agcaagcaag ccaacaccac gacaaccaga acgacccatc
     3361 gaaacttttt cactccaatc tccgtttcac ctaccttatg tcattaaact agcatgcgtt
     3421 agttaaacag cgcctaacta tgtcgatatt cgtgcttttt cggaatgagt cgcttgcttc
     3481 agcgcatatt gccgctacga ttaagcgaac ataaaaagag aagaggttgt aatgcgattc
     3541 atcattcgca cagttatgct gatagcactg gtatggatag gattattact cagcggatat
     3601 ggcgttttga ttggaagcaa agagaatgcc gcaggattag gtttgcaatg tacctatctg
     3661 actgccagag gcaccagcac ggtacaatac ttgcacacta aaagtggttt tttggggata
     3721 acagattgcc cgctgttacg caaaagcaat atcgtggtcg ataatggtta agtgtaataa
     3781 ataaccagat tctcactcat gagtaaaata ctcttatagc aagcgcgttt aatataacgc
     3841 caccttcaac tttatttttc tcatgctaaa taaagaacct gtctgtttga tattccgaca
     3901 ggttcttact ctcatcagaa cggatagtcg tgataaccca tttgtgcaga aattttgcgc
     3961 gctgcggtat gcaacattgc gacatattcc tgtaaacgct cttcagagaa acgcaacgtc
     4021 gggaaggaga tgctcaaacc ggcaatgacc acgccaaagc gatcaaatac cggaaccgca
     4081 atgcatcgca gtccttcttc ctgctcttcg ttatcttcgc catacccctg ctcgcgcacc
     4141 tggtccagaa ccggtaataa cgcttctgta ctggtgatgg tccgctcggt actgcgttta
     4201 tactccacgc cctcaagaat ttgcttcact tcatcgcgat cgcgccatgc cagcagcacc
     4261 ttaccaatcg cggtgctgta cagcggatta cgacgcccaa tccgtgaata catgcgcaaa
     4321 ttgtacatag agtcaatttt gtgaatgtaa acaatactgt cttcgtccag tgcgccgagg
     4381 tggatagttt ctttggtcag gcgggagatc tcacgcatct ggatatctgc gctacgaatt
     4441 aaatcgacgt tttgtaacgc gcgagcgccc agttcaaaca atttcagggt cagggaatat
     4501 ttctccgact ccccttcctg cgccacataa cctaaggttt tcatggtctg taaaaagcga
     4561 taaacggtgc tttttgacat catgacgcgc tgcgacagct cggttatccc tatttcgcgc
     4621 tcttcaccca gcgcctgcaa aatgccaaaa acttttagca cggaagatac agaatcaggc
     4681 tgtttatcca gatctgcgtt agccatttat cacctcattg cgagtgtttt ataaaaatca
     4741 gaactgtttt ttattataat ttcgcaccag ggtggtcgca atccatcttt tgccggttag
     4801 ttacaattct gcgacatcca ccgtgaatat cagtgctaga atcatacccc tgttgattat
     4861 tcactaaaga tataaaattc ctatgccaaa agttcaggcc gacggcctgc cgttgcccca
     4921 gcgatacggt gcgatattaa ccattgtgat tggtatttcg atggctgtcc ttgacggcgc
     4981 aatcgccaac gtcgccctgc caacaatcgc cacggacctt catgccacgc cagccagttc
     5041 catctgggta gtgaacgcct atcaaatcgc cattgtcatc tccctgctct cgttttcgtt
     5101 tctgggcgat atgtttggct atcgacgtat ttataaatgc ggtctggtcg tttttctgtt
     5161 gtcttcactg ttctgcgccc tttctgattc gctgcaaatg ctcacccttg cgcgtgtcat
     5221 acaaggtttc ggcggtgcag cgttgatgag cgttaatacc gcacttatcc gcctgatcta
     5281 tccacaacgt tttctgggta gagggatggg cataaactcg tttattgttg ccgtctcttc
     5341 tgctgccggg ccgacaattg ctgcagcaat cctctccatc gcatcctgga aatggttatt
     5401 tttaatcaac gtaccgttag gtattatcgc cctgcttctg gcgatgcgtt ttctgccacc
     5461 caatggttct cgcgccagta aacctcgttt cgacctgccc agcgccgtga tgaacgcgtt
     5521 aaccttcggc ctgcttatca ctgcgttgag tggtttcgct caggggcaat cgctgacgtt
     5581 aattgctgcg gaactggtgg tgatggttgt tgttggtatt ttctttattc gccgccagct
     5641 ttctcttccc gtaccgctgc taccggtgga tttactgcgt atcccgctgt tttcactttc
     5701 tatttgcaca tctgtttgct ctttctgcgc acaaatgctg gcaatggttt ccctgccctt
     5761 ttacctgcaa accgtgctcg ggcgtagtga agtcgaaaca ggtttacttc tgacaccgtg
     5821 gccgttagca acgatggtga tggctccgtt ggcaggctat ttgattgaac gcgtacatgc
     5881 aggattgctg ggtgctttag gattattcat catggctgcg gggctttttt ccctggttct
     5941 gctgcccgcg tcacctgcgg atatcaatat tatctggccg atgatcttat gtggcgctgg
     6001 atttggcttg ttccagtcac ccaataacca caccattatt acctccgctc ctcgcgaacg
     6061 tagcggtgga gccagtggca tgttaggaac ggctcgtcta ctgggtcaga gtagcggcgc
     6121 ggcgctggtg gcgctgatgc taaatcagtt tggagataat ggtacacacg tctcgctgat
     6181 ggctgcggct attctggcag tgattgctgc ctgtgtcagt ggtttacgta tcactcagcc
     6241 acgatccagg gcataaaaaa agcgcgtcga tcaggacgcg ctttttagtg tttacttcat
     6301 aatattactt caggtattca cccgtacgca gagcttcaat tcgtttatcc agcggcgggt
     6361 gagtcatgaa caactcactg agcgatttcg acttaccgtt aatgcagaaa gccatcatgc
     6421 tggttgcttc ttgcggttca tagctggttt tcaggcgctg cagcgcggca atcattttct
     6481 cgcgaccaac cagttttgcc gaaccggcat cagcatggaa ttcacgatga cgcgagaacc
     6541 acatggtgat aatgctcgcc agaataccaa acaccagttc cagaaccgtt gcaaccgcaa
     6601 agtagatcag cgggttgccg ttgctctctt caccttcatc acgatttccg cccataaaac
     6661 ccgcggcaag ctgcgccaag atacgggaaa taaagataac gaaggtgttc accacgccct
     6721 gaatcagcgt catggtgacc atatcaccat tggcgatgtg gctgatttcg tgagcaatta
     6781 ccgcctcggc ttcatccggg ctcatgttct gcagcaaacc ggtgctgaca gcaaccagag
     6841 aggcatcacg gcgcgcaccg gttgcaaaag cgttgatgtc cggcgcatgg tagatagcca
     6901 cttgcggcat agcgatcccc gcctgacgag cctgggttgc tacagtattg accagccaac
     6961 gttccctttc gttacgcggt tgctcgatca cttccccgcc aacagatcgt aatgccatcc
     7021 atttggacat cagaagcgaa acgaaggaac caccaaaacc gaacagcaag gccatgatca
     7081 tcagcccctg aacgctgctc gactgtatcc ctgtcaggct cagtaccagc ccgaaaacga
     7141 ccattacggc caggttcgtt agcaggaaga gcgcgattcg catcataatt ttctttttac
     7201 ctcagtttaa caaaacgcaa tatgcgatac ccacatcgta tgggttacgc gactattttc
     7261 aagtctggat agtgcgtaag tcaccagaaa gacacaactt tacattttgt agcatctgat
     7321 ttacggcatc ttgccgctgt taaaaaatca ggcacaattt cttgtgcctg attgatatta
     7381 cttgacgggt gcgggttgtt ccgcgggtct ggctttttca agcttcgcca gatcgagtgc
     7441 gatattcacc gtctcatcca gataaggatc cggctcctgg taatctttcg gtagatcatc
     7501 cagtttcttc aactccggtt taccttcgcg tttaaagcgt tcgttcaaac gcgccagacg
     7561 cgtcgcatca tcttcattat tctctttctc acgcacagcg taattcagag aaacgatatt
     7621 gcgcttgtcc ttcatagcgt tgaagcgcgc gatatccttc atgatgttct ggaactcagg
     7681 atctttcgcg atacgcgcat tatgttcctt cagtagctcc ggttcaaagg ccgttaaatc
     7741 tcctgatttc acataagtcg cggcatcaat gctatcccac ggcagcgcgt tatcttcgaa
     7801 tttctcaccc gtttccgttt cttcattacc cgtcggcatg atgatgtctg gcgttacgcc
     7861 tttacgttgc gtactgccgc cgttaacgcg atagaatttc tggatcgtgt actgcacaga
     7921 acccagcgct ggccattcag gacgtaacat ctgatcgtag atacggttta atgaacggta
     7981 ttgctgaacg gtgcctttac caaacgtcgg ttcacccaca accagcgcac gaccgtaatc
     8041 ctgcattgcc gcggcaaaga tttctgaagc cgaagcactg aagcggtcaa ccagtaccac
     8101 cagcgggcct ttatagaaaa cctgtccgtc ggtatcgctg tcttcacgaa ctttaccgtt
     8161 gttatcacgg acctgaacaa taggacccgc aggaataaac agacctgaga gcgataccgc
     8221 ttcggtcaac gccccaccgc cattgctacg caggtcgatg atcacactgc tgacattctg
     8281 tttttccagt ttctgcagtt gtactttgac atcgtctgtc aaacccacat agaagcccgg
     8341 aatatccagt acgccgactt tctctttacc gacggtcttc accgacattt taaccgcgcg
     8401 gtcttcgaga cgaatacgtt cacgggtcaa tgttacagtg cgggtcttgg tccctttacc
     8461 agcaggtaaa atttccagac gaactttact gcccttcggc cctttaatta aggcaaccac
     8521 atcatcaaga cgccagccaa tcacgtcaac catcggcttg cctgtttgac caacaccgac
     8581 aattttgtca ccaacgctga tagctttact cttcgctgcc ggaccacctg ccaccatcga
     8641 attgataacg gtgtagtcat catccatttg cagcactgcg ccaatacctt ccagcgacaa
     8701 actcatttca gtgttgaact gttcggtatt acgcggggaa agatagttgg tatgcgggtc
     8761 gatttcacgc gcaaacgccg tcattgccag cgagaaaaca tcttcgctgt tggtttgcgc
     8821 cagacgacga atggcaaatt tgtagcggcg agtcagggtt tcacgaattt ctttatccgt
     8881 ttttcctgcc agcttcaggc ttaactcgtc gaatttgact ttactgtccc acagcgcgtt
     8941 caactcagcc tcgtttttcg gccagggcgc tttgctgcgg tcaaggttat aagtgtcgtt
     9001 gccggtgaaa tccatcggct tttctagtac cgacaaagcg tactggtaac gctcaaaacg
     9061 gcgcttttgc gccagattgt agagatcgta gaaaacgtcg agtttgcctg aacgcagttc
     9121 atcgcctaac tcggttttct ttttcgcgaa ctgttcaaca tcgcttgcca acagcacgtt
     9181 gtggctgtaa tcgagcagat tcaggtagcg gtcaaagatt ttggccgaaa atgcctgatc
     9241 gaggtcgaac tggcgataat gagaacgggt gaagcgcgac gttacgcgct cactcaccgt
     9301 cgcatgctgc gtctcttcct ttaataccgg aatttgatca gcacgcgtga tatcttctac
     9361 agcgaaggtc tggcctgcta ttgcaagcag gccagctaac gcggtaagcc taaaaaacat
     9421 gttcatgcct ggcccggcct ccgtttcaga acaccaggtg ttctgcgcgc acaatcaaag
     9481 acatacccga attcagctgg acgcggacgc cgtctttggt gatttctaat acggtggcat
     9541 ccatcgcgtt ttgacccgct ttcaccttca gggcttgtcc gacagtcaga gctgaaatgt
     9601 cagaaactgg ggtgtgctgt tcttcgcgag gtgcttttgc tgtttttggc gctttctcta
     9661 ccggcttttg cgcacgaggt ttacgttcag cgccttcttt gcggcgtggc gtggtcggac
     9721 gtggcttgcg ttcgcgacgc ggtgcgtctt ctttctcacc agcagctgcg gcagcttcgc
     9781 gttttttcgc ttgctgttca gcacgctgtg cctgaacacg cgctttcgct tcttcaagct
     9841 gcttgcgagc atgctctaca tgttgctcgt ccagctcacc gcatgggttg ccgtcaagat
     9901 cgacacgcgt tgcgccgggt ttaacaccgt aaagataacg ccagctcgaa gtgtagagac
     9961 gtaaagcgga tcgtaattgc gttttgctca ggttcatttc cccggcaaca cgatcgacca
    10021 aatcctgaaa aataccgatt ttcagcggac gcgcttcacc ttccgcactg aaacagtggg
    10081 gaaaacgttc ggccagaaac gcgattactt ctttactgct attcaactta ggttgatttt
    10141 ccatgaaatt tcctgattac aacggacgta gccaacaagc gcaggcatga acaggcgtca
    10201 ttataatgac gctatcagta aatgctacgt tatccgttga ttatcctgcg acgctcgcaa
    10261 agaatttttt gtaatccgtc gttgcaagca ctttttcaag ctgtgccaca agctgacgta
    10321 agccttgctc gtcctcgtct gtaaagcgac cgaagacggt actatcgatg tcgagaacac
    10381 caataatctg atttttcacc accagcggca gaacaatttc agaattactc gccgcatcac
    10441 aggcaatatg cccgtcaaac gcatgaacat cctcgatacg ctgcacttga ttgcgggcaa
    10501 ccgcagtgcc gcacacgcca cgcccgacgg gtatccggac acaggcaatt ttgccctgaa
    10561 atggtccgag caccagtgta tcgtcctcaa gcaaataaaa acctgcccag tttacgtcag
    10621 tgagacgctc atataacaac gcactggtgt tcgcaagcgt tgccagaaaa ctggtttctc
    10681 ccgccatcag cgcgttaaag tcgcgattta aatccgcgta aaattctgtt ttgttcatta
    10741 tataatcact tggttgtctt acctggaact gccagcctat taaaataagc attaaatgcg
    10801 ttaatgctca agatcattcc catcatgggt taagattaat gttaattctt attacatttg
    10861 gcacgtcatg gctcttaaca caccacaaat tacgccgaca aaaaagataa cagtgagggc
    10921 aatcggcgag gaactgccgc gtggtgatta ccaacgttgc ccgcaatgtg acatgctgtt
    10981 tagcctgccc gagataaatt ctcatcaaag tgcctattgt ccgcgctgtc aggcaaaaat
    11041 tcgtgacggg cgcgactggt cgctaacgcg cctggcggca atggccttca ccatgctgtt
    11101 gctgatgccg tttgcctggg gcgaaccgct gttgcatatc tggctgttag gcattcgtat
    11161 tgacgccaac gttatgcaag gcatctggca aatgaccaaa cagggcgatg cgataacggg
    11221 gtcgatggtc tttttctgcg ttattggtgc cccgctcatt ctggtgacct ccatagctta
    11281 tttatggttt ggtaaccgac tgggaatgaa tctacgtccg gtactgctga tgcttgagcg
    11341 acttaaagag tgggtaatgc ttgatatcta cctggtcggc attggcgttg cttctataaa
    11401 ggtacaggat tatgcccata tccaggcggg tgtcggcttg ttctcttttg tggcgttggt
    11461 gattttaacg acggtgacgt tgtcacatct taatgtcgag gagctgtggg agcgatttta
    11521 tccgcagcgc cccgctacgc gtagggacga gaaacttcgt gtctgtcttg ggtgccattt
    11581 taccggctat cctgatcagc gtggtcgctg cccgcgttgc catattccgc tacgcctgcg
    11641 tcgccgtcat agtttgcaaa aatgctgggc ggcgctgtta gcgtcaatcg ttttgttgtt
    11701 acctgccaac ctgttgccta tttctatcat ttatctgaat ggtggccggc aggaagatac
    11761 gattctttcc gggattatgt cgctggcaag tagcaacatt gccgttgcgg gaatcgtgtt
    11821 tatcgccagt attctggtac cgtttactaa agtgatcgtc atgttcactt tactgttgag
    11881 cattcatttt aaatgccagc aaggtttacg cacacgcatt ctgttactgc gaatggtgac
    11941 ctggattggt cgctggtcga tgctcgacct gtttgtcata tctttaacca tgtcgctgat
    12001 taatcgcgat cagatcctcg cttttactat gggaccggct gcgttttatt tcggcgcagc
    12061 ggtaattttg actattcttg ctgtggaatg gctggacagc cgcttacttt gggatgcaca
    12121 tgagtcagga aacgcccgct tcgacgactg aagcgcagat taaaaataaa cgccgtatct
    12181 cacctttctg gctgctgccg ttcatcgcgc taatgattgc cggttggctg atttgggaca
    12241 gttatcagga ccggggtaat accgtcacca tcgactttat gtcggcggat ggtattgttc
    12301 caggccgtac gcctgttcgt tatcagggcg ttgaagtcgg aacagtgcag gatatcagcc
    12361 tcagcgacga tcttcgtaag attgaagtca aggtcagcat caaatccgat atgaaagatg
    12421 cgctgcgcga agagacacag ttctggctgg tgacgccaaa ggcctcgttg gcaggtgtct
    12481 ccgggctgga cgccctcgtc ggtggtaact atatcggcat gatgccaggt aaaggtaaag
    12541 agcaggatca ctttgtcgca cttgataccc aaccgaaata tcggctggat aacggcgatc
    12601 tgatgatcca cctgcaagcc cccgatctcg gttcgctgaa cagcggttca ttggtctatt
    12661 tccgcaagat cccggtggga aaagtctacg actatgccat caatcccaac aagcaaggcg
    12721 tggtgattga tgtcctgatc gagcggcgtt ttaccgacct ggtgaaaaaa ggtagccgtt
    12781 tctggaacgt ttccggcgtt gatgccaacg tcagtatcag tggcgcgaag gtgaaactgg
    12841 aaagtctggc ggcactggtt aacggtgcga ttgccttcga ttcacctgaa gagtcgaaac
    12901 ctgccgaggc ggaagatact tttggtctgt atgaagatct ggcccacagc cagcgtggcg
    12961 taataataaa actggaactg ccgagtgggg ccggattaac cgccgactcg acgccgttaa
    13021 tgtatcaggg gctggaagtc ggacagctaa ctaaactgga tttaaatcct ggtggtaaag
    13081 tcactgggga aatgaccgtt gatcccagcg tcgttaccct gcttcgtgaa aatacccgca
    13141 tcgaattacg taatccgaaa ttatccttta gcgatgccaa tctcagcgcc ctgctgactg
    13201 gcaaaacctt cgagttggta cccggcgatg gcgagccacg caaagagttc gttgttgtgc
    13261 caggcgaaaa agcactgctg catgaacctg atgttctgac gctgaccctg accgcgccgg
    13321 aaagttacgg tattgatgcg ggtcagccgc tcattcttca cggcgtgcag gtaggccagg
    13381 ttatcaatcg taaactcacc agcaaaggcg tcacctttac cgtcgccatc gagcctcagc
    13441 atcgagaact ggtaaaaggc gatagcaaat ttgtcgtcaa cagccgtgtc gatgtgaagg
    13501 tggggctgga tggcgttgag tttcttggtg ccagcgcctc agaatggatt aacggcggga
    13561 tacgtattct gccgggcgat aaaggcgaga tgaaagccag ctatccactg tatgccaatc
    13621 tggaaaaagc gctggagaac agccttagcg atttacccac cacaaccgtg agtttgagtg
    13681 cagagacgct gccggatgtg caggcaggat cggtagtgct gtaccgtaaa tttgaagttg
    13741 gtgaagttat taccgtgcgt ccgcgagcta acgcgtttga tatcgatctg catattaagc
    13801 cggagtatcg caaccttctg accagcaata gcgtgttctg ggcagaaggc ggggcgaaag
    13861 ttcagctgaa tggtagtggc ctgaccgtac aggcatcccc gctctccaga gcattaaagg
    13921 gagccattag cttcgataac ctcagcggtg ccagcgccag tcagcgtaaa ggcgataagc
    13981 gtattctgta tgcttccgaa acagcggccc gtgcggttgg cgggcagatt acgcttcacg
    14041 ctttcgatgc cggaaaactg gcggtcggga tgcctattcg ctatctcggt attgatatcg
    14101 ggcaaatcca gacgctggat ctgattaccg cgcgcaatga agtacaggca aaggcggtgc
    14161 tctatccgga atatgtccag acctttgctc gcggtggtac gcgcttctca gtggtcacac
    14221 cgcaaatttc ggcagctggc gttgagcatc ttgatactat cctccagccg tatatcaacg
    14281 tcgaaccagg ccggggcaat cctcgccgcg actttgaatt acaagaggcc accattactg
    14341 attcgcgtta cctggatggc ttaagcatta ttgttgaagc gccggaagcc ggttcgttag
    14401 gcatcggtac gcctgtgctg ttccgtggtc tggaagtcgg tacggttacc ggaatgacgc
    14461 tggggacatt gtcagatcgc gtgatgattg cgatgcgcat cagtaaacgc tatcaacacc
    14521 tggtgcgtaa caattccgtc ttctggttgg catcgggtta cagtctggac tttggtctga
    14581 cgggcggcgt agtgaaaacc ggcaccttta accagtttat ccgtggcggc atcgccttcg
    14641 ccacgcctcc gggtacgcca ctggcaccga aagcccagga aggcaagcac ttcctgttgc
    14701 aggaaagtga accgaaagag tggcgtgaat ggggaactgc gcttcccaaa taatgcccac
    14761 tgctccggcg tgcctgcgcc ggagcgttta tgctaaactg cgcgcctgtt tttttgccag
    14821 tggtatatgc tcgtggccca acacaccgtt tatttcccgg acgcctttct gacgcaaatg
    14881 cgcgaagcga tgccttcgac gctctctttt gatgattttc ttgctgcctg tcagcgcccg
    14941 ttgcgccgca gcattcgcgt taatacgctg aaaatctccg ttgctgattt cctgcaatta
    15001 accgctcctt atggctggac gcttacgcca attccgtggt gtgaagaagg tttctggatt
    15061 gaacgcgaca atgaagatgc attgccattg ggtagtaccg ccgagcattt aagtggcctg
    15121 ttttatattc aggaagccag ttcaatgttg cccgtcgccg ccttgtttgc tgacggtaat
    15181 gcaccacagc gggtgatgga tgtcgctgct gcgccaggct ccaaaacgac gcaaattgcc
    15241 gcgcggatga ataacgaagg ggcaatcctt gccaatgagt tttccgccag tcgggtaaaa
    15301 gtgttacatg ccaatatcag ccgctgtggt atcagtaatg ttgcgctcac acattttgat
    15361 ggccgcgtgt ttggtgcggc attgccagaa atgttcgatg ccattttgct ggacgctccc
    15421 tgctccggcg aaggcgtggt gcgtaaagat cccgatgcgc taaaaaactg gtcaccagaa
    15481 agcaatcagg aaatcgcagc tacacaacgg gagcttatcg acagcgcctt tcatgcatta
    15541 cgtcctggtg gtacgctggt ttactcgacc tgtaccttaa accgggaaga aaacgaagcc
    15601 gtttgcctgt ggcttaaaga gacttaccct gacgcagtag agtttttacc acttggcgat
    15661 ctcttccctg gtgcaaacaa agcgctgacc gaagaaggct ttttgcatgt tttcccacaa
    15721 atttacgact gcgaaggctt cttcgttgct cgtctgcgta aaactcaggc cattcccgcc
    15781 ttacccaccc caaaatacaa agtggacaat ttcccgttca gcccggtgaa agatcgcgaa
    15841 gccgcacaaa ttcgtcaggc ggctgcaggt gttggcttaa actgggatga aaacctgcgc
    15901 ctctggcagc gtgacaaaga agtgtggttg ttcccggtgg gcattgaagc cctgatcggt
    15961 aaagtccgat tttctcgcct ggggattaaa cttgccgaaa cgcacaacaa aggttatcgc
    16021 tggcagcatg aagcggttat tgcccttgcc tcccccgaca acgagaacgc ttttgaactg
    16081 acaccgcaag aagcggagga gtggtatcgc gggcgcgatg tttacccgca agccgagcca
    16141 gtggcggatg acgtgttggt tactttccag catcagccga ttggtttagc caaacggatt
    16201 ggttcgcgac tgaaaaacag ctacccgcgt gaactggtgc gggacgggaa actttttacc
    16261 agtaacgcat gacagcgcac aaaaaaagcg cactttttga ctggcacatt cggcggcctc
    16321 aactaggctg aaaaatggtg cgatcggact ggtcgtacca caatcggcag ctaaatggag
    16381 agcacgaaga tgaaaaccag tgtgcgcata ggcgcttttg aaatcgacga cggcgaatta
    16441 cacggtgaat cgccgggtga tcgaacgtta accattcctt gtaaatctga ccccgattta
    16501 tgtatgcaac tggatgcctg ggatgctgaa accagtatcc ctgccctgct aaatggcgaa
    16561 cactctgtcc tttaccgtac ccgttacgat caacaatctg atgcctggat tatgcgtctt
    16621 gcctgatcca aaaagaaccc gtcggcatgg cgggttattt gtcctggtta ttcccccgtt
    16681 gtaaaatctc tcctaaactt aacggtacgg caccacactt cggggatgaa atgttcgcgc
    16741 tggtactttt tgtttgctac ctggatggcg gttgtgaaga tattgttgtg gatgtctaca
    16801 acacggaaca gcagtgtctt tattctatga gcgatcaacg gatccgccat ggcggttgtt
    16861 ttccgattga ggattttata gatggtttct ggcgatcagc acaggagtat ggtgattttt
    16921 aattattgca attgcacgaa agtcagttcg cccccaaaga cagcgccggt atcaatataa
    16981 tgcaggttgc caatatccac gcgatggcgc aacggtgtat gaccaaacca gaaatgatca
    17041 gcacctgtaa ttccctgccc tttttggcgt tcacttaatc gcgagcggct ccacaagacc
    17101 tgatgcaaat caacgtcctt ttgccattca taaacatcat ctggataatc ggcatgagca
    17161 ataacatgtt tgccggtgcg gctgtgtact tcaagaataa agggcaaatg ctgacatttt
    17221 tccagcgccg ttttcgcttg tttctgttga ttatctgcca gcgcaataaa ccagtcgccg
    17281 ccattcatca accacaaaga catctgctgg gatgccagcg catccatcgc catctgttca
    17341 tgattgcctc ttaccgcacg aacccaatgt tgttccagta actgcagaca acgtaaactt
    17401 tgcggcccac gatcgataac gtctcccact gagataagta aatctcgcca cggatcaaaa
    17461 cgacaatgcc ataatttgcg gcgcaactgc tcaagacaac cgtgtatatc gccagaaagc
    17521 cagatatgtc gccattgatg acccgcaatt ctctgataaa cgggcgcagg ctgtttcatc
    17581 aatattttcc tcccgcgcta aagatcacat aatcttaaca agaatgttaa aaaacgctgg
    17641 actcagacag tagagtgtgt gttatggttg actataaagt cagcgaagga aatgcttctg
    17701 gcttttaaca gataaaaaga gaccgaacac gattcctgta ttcggtccag ggaaatggct
    17761 cttgggagag agccgtgcgc taaaagttgg cattaatgca ggcttagttg ccttgccctt
    17821 taagaataga tgacgacgcc aggttttcca gtttgcgtgc aaaatggtca ataaaaagcg
    17881 cggtggtcat cagcttaaat gttaaaaacc gcccgttctg gtgaaagaac taaggcggtt
    17941 tttttattgg aaatcaaaag gctattttag gtaattaaca gagtttttca gctcgttcta
    18001 taaacggtgc cagactcatt ttttcgccgg gattgttggg atcatcaatc tgaatcaccg
    18061 aaatgggttg ggctttagtc ttcccactgg caacttcctt ctgtgcgaca tcgtttaaag
    18121 gatactgcac gagggtactt gggttgatga catacaaagc attacccggt cggcaagtca
    18181 gcatcacctc ttcgcgatta aacgcccatt tgtctttacc cacttcaaaa cggctgacgg
    18241 taatcacctg cggtgcagcc agcgccgctg cagaactggt gagtaacaga aacgccagaa
    18301 tacttttttt catcatttcg taatccatca aaaaggttcg agagtcgcaa gcaggctgat
    18361 gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca caaaccaggg
    18421 tgccgcacga tcttcatcct gcctcatgcg cggtacaaga acataacgat tagccagcgc
    18481 aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc cccagtaagt
    18541 cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc ttgccagtac
    18601 gccgatcacc gcaaaatgcc cgcaccaaga aaaacgcatc agcgcctgaa tagcctggtg
    18661 tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc ccccaaacca
    18721 cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg ccgttacccc
    18781 ttcattcagc gtcgcatgcc ccactcccgc cagcagaata aattgcgcgg tagtgagcat
    18841 aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg ttacgagggc
    18901 gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag cctgccaaat
    18961 gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa cggcgagcat
    19021 tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca gaaaacgctt
    19081 cgccagcaaa cgacgaatcg ttaagggtga cagccaggca ccgtacattg cgaagccaaa
    19141 gaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa acgccagcat
    19201 gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca ccgaaacaac
    19261 atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa caatcagttg
    19321 cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt tttttggccc
    19381 cgtgattttt gcaccactga atcctgtttc aacaccttcc gagaagttta aagtgattgc
    19441 ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta aatgcgcatg
    19501 tgcccagacg gaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt agcgaaggga
    19561 gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta cagagcataa
    19621 ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatccag gctcactctt
    19681 agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct ggcaaaactg
    19741 gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt ggcatttaaa
    19801 gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc tgaacatttg
    19861 cgcagctggt ttcgcgagcg tcttatcgcc caccgtttgg cttcggtcaa tctgtcacgt
    19921 ttaccttacg agcccaaact taaataaaac ttatacagag ttacactttc ttacataacg
    19981 cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat tttgaaaagg
    20041 aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt aaaacctcac
    20101 tcaccgaaca ggtagctcat catctggagt ttgtgagggc cgccgccagg caacaatgtc
    20161 agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcacga cgtgcccttc
    20221 ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg acctggcgaa
    20281 tgaccatcat agccggcctt cccgttgaat ataacgatcg ctttattcgt ggaattgcgg
    20341 tgttcgcacc gtggcgaaaa acgccgggga tctaccatca gagtcatggt gcatgtctgg
    20401 gccgtcgttc cagaaccatc acggtggtcg atgaacaacc gcaaggtatg gatatggacc
    20461 cgacctgttc actgtttacg acagggcaat gtctggggga acccgaccta ctggcatcta
    20521 cccgccgctt acaatttttt tcacatcagt actccattgc cgtgctgatg gcaaatgccc
    20581 gtggtaacag tgcactatgg gatgaacatg gtcgtctcat cgttcgcgcc gatcgtggtt
    20641 cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc attccattac
    20701 gctaggcttt ttcggcctgg agcatgccat gttgcgcatt atcgatacag aaacctgcgg
    20761 tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa aaatcgtcaa
    20821 ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga tggcgattca
    20881 tcgcatcacc gaagccatgg tagccgataa accgtggatt gaagatgtga tcccacacta
    20941 ttacggtagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg tactgcctga
    21001 gatgcccggt gagtggattt gcactatgaa actggcccgt cgtttgtggc cagggatcaa
    21061 gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc cgccgggcct
    21121 gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta tcgatattat
    21181 gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc cgtcgttgat
    21241 gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg ccgaacgcga
    21301 tccgggctat ctgcgctggt tgtttaataa cctggacagc atgagcccgg agctgcgttt
    21361 aacactgaaa cattatctgg aaaataccta atccgcaggc gtagcgggta atgttccctg
    21421 cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt taaagcggcc
    21481 agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat ggttatcggt
    21541 tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca cctgagaatc
    21601 gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt tgtcatacgg
    21661 gctgtagctt ttcatatact cgtaatattg cggatcctgc ggattccccc actcttcaaa
    21721 ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat caacaaacgg
    21781 tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa cgcccatcaa
    21841 catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat agcccagttt
    21901 taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct tcagaaattt
    21961 accgtcttcg taccattgtt gccccagctc accgccgccg cgaacatgga cgatggcgta
    22021 gacaaaacca cgatctaaca aactcaagcg gctaaaactg aaatcggcat caatacttgc
    22081 gccgtaagaa ccatagccat acaccagcaa cgggttgtgc cctttgcgaa aatgtttgcg
    22141 atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc acaggtgttc
    22201 actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata cacgacgctc
    22261 accggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag aataaccata
    22321 acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat aggctggatc
    22381 atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca aactggttaa
    22441 cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc cttccagcat
    22501 gatgttttcg cgtggcggaa ttaactcttc ccactgttgc tcatcacgca tacgggtacg
    22561 gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat gctggtagtg
    22621 atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct cggcatcggc
    22681 catttccgcg tccagcaggc gaacttcact gctggtggcg ctggccagat gaatgactac
    22741 atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt tttcttcgta
    22801 aatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct gataaggcag
    22861 cagcgtcacc ggatgcttgc gtacatagta gaaagtccag gagtcatttg cccagacaaa
    22921 gctgggttca acgttatcca gcagttccgg gtaccagtta cctgtttcca gattacgaaa
    22981 acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa tggtgttatc
    23041 gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagccgcgc gcttgttggc
    23101 atcgagcaat atttcccact cgtcccactc ttcactgaac gccgattgac gctggtagat
    23161 agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt tgatgtaggg
    23221 cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc gatcctgcaa
    23281 ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca ggtagtccag
    23341 gacttccggc tgagaacgcg tatcgtcccg cagccagtag taattatcga tgcgcgtatc
    23401 gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt tattgttctt
    23461 tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc gaaacagggc
    23521 agtcattgct tagatgatga caggtaatgg cgcgaatatc gaatgttatg cagacagaga
    23581 aaatcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc aacttcaggc
    23641 tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga tcgcgttcaa
    23701 tgccttcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc aggtaactgc
    23761 gttccataaa atggtcaata tcaatagccg cgcagctcag gaaatagatt tccagcgcct
    23821 cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt tcgattgcct
    23881 gctcaatgag tacccgcccc tgctcttcca cgccggcttc acgcaattgc tggtcgattg
    23941 tcgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagact agcgcaagga
    24001 tcaaacgttc cgtacgctca tccagcggcg tactttgcgc gccaaactgc ggttcgtcct
    24061 gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc gctaccgctc
    24121 cgccgccaac cagcaacgcg ttggtgccat atttcgtaag aagtttacgt gctgatttat
    24181 ttgcgaccag cagtccagcc agtccaccta atgcgcccgg cactaaaagt ttgcccaatc
    24241 cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat tgcagttgat
    24301 ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta gcatagtgga
    24361 tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg ggcggttggc
    24421 cgttattcaa agaaattcgc gccaggtaag gtgtttactg tatatcaggt gataagggta
    24481 ttttaggtga aagtagtttg acgttttgag tggcattgtt tgatgctgat tgcccgatgc
    24541 gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tttggggcgg acaaggcact
    24601 cgcgcccatc cgccaacggt caatcgaatt atcagaaccc attaacgccg ctgatattcc
    24661 gccattcccg ctttgcagtc cacgctgacc tggtaatgaa tatcggcact tttaccacgc
    24721 acggttagcg gtactgacca tttatcatct ttaccctgaa tgtcctgcaa actgacccaa
    24781 gccacgggat cggcctgacc gacaattttt tgatcatctg cccagcgcgc cacgcgattt
    24841 tgttgataat cacgttttac actcgcggca attccggcag catccagacc ttcacacttt
    24901 gggaaagtga ccgacttgct ggtttcatta ttggcagcga aaactgatgc gcaggcagaa
    24961 accaacaaca gccctaaaaa cgcccctctt tttttcatgt ttttctccat agcacaatga
    25021 ttcaggagaa agcatggtac aaattgtcag gagcgcaagt tgcttcaggc cgcgtgtgag
    25081 gcatcttcct cttcggattc agcaccgacg acctgtgggg acggtaattt gccggttttc
    25141 agaatggtgc tcagcacatc tttttgttct gccagccata gcgaaagggc ttcacgttgc
    25201 tcgtcttcca tttgcactgg agaatttgtc agccaggtgt caagcagatc cgccgtatca
    25261 agcattttgt cataagcatc ggcttccttt ttgctggtaa acgacatttt ctcttcgccc
    25321 tcacgaatga ctacgtattt aacttcaacc gccatttgca gcctctcata ataactgtga
    25381 ttttatacag tatatttctt ttcggttgag aaatcaacac cagcaataaa gacacacgca
    25441 aacgttttcg tttatactgc gcgcggaatt aatcagggga tattagttat gacgttatta
    25501 ggcactgcgc tgcgtccggc agcaactcgc gtgatgttat taggctccgg tgaactgggt
    25561 aaagaagtgg caatcgagtg tcagcgtctc ggcgtagagg tgattgccgt cgatcgctat
    25621 gccgacgcac cagccatgca tgtcgcgcat cgctcccatg tcattaatat gcttgatggt
    25681 gatgcattac gccgtgtggt tgaactggaa aaaccacatt atatcgtgcc ggagatcgaa
    25741 gctattgcca ccgatatgct gatccaactt gaagaggaag gactgaatgt tgtcccctgc
    25801 gctcgcgcaa cgaaattaac gatgaatcgc gagggtatcc gtcgcctggc ggcagaagag
    25861 ctgcagctgc ccacttccac ttatcgtttt gccgatagcg aaagcctttt ccgcgaggcg
    25921 gttgccgcca ttggctatcc ctgcattgta aaaccggtga tgagctcttc cggcaagggg
    25981 cagacgttta ttcgctctgc agagcaactt gctcaggcat gggaatacgc tcagcaaggc
    26041 ggtcgcgccg gagcgggccg cgtaattgtt gaaggcgtcg ttaagtttga cttcgaaatt
    26101 accctgctaa ccgtcagcgc ggtggatggc gtccatttct gtgcaccagt aggtcatcgc
    26161 caggaagatg gcgactaccg tgaatcctgg caaccacagc aaatgagccc gcttgccctt
    26221 gaacgtgcgc aggagattgc ccgtaaagtg gtgctggcac tgggcggtta tggtctgttt
    26281 ggtgtcgagc tgtttgtctg tggtgatgag gtgattttca gtgaggtctc ccctcgtcca
    26341 catgataccg ggatggtgac gttaatttct caagatctct cagagtttgc cctgcatgta
    26401 cgtgccttcc tcggacttcc ggttggcggg atccgtcagt atggtcctgc agcttctgcc
    26461 gttattctgc cacaattgac cagtcagaat gtcacgtttg ataatgtgca gaatgccgta
    26521 ggcgcagatt tgcagattcg tttatttggt aagccggaaa ttgatggcag ccgtcgtctg
    26581 ggggtggcac tggctactgc agagagtgtt gttgacgcca ttgaacgcgc gaagcacgcc
    26641 gccggacagg taaaagtaca gggttaaacc cggcaaataa acgctacaaa aatgcccgat
    26701 cctcgatcgg gcattttgac ttttacagct tagcgccttc tacagcttca cgcgccagct
    26761 tagtaatgcg gtcgtaatcg cccgcttcca gcgcatctgc cggaaccaac caggaaccac
    26821 cgatgcacag cacgcttttc agcgccaggt agtcacggta gttagccgga gaaataccac
    26881 ccgtcgggca gaaacggacc tgggagaacg gacccgcgat cgcctgcagg gctttcacgc
    26941 cgccgttagc ttcagccggg aagaatttga actctttcaa accgtagtcc atacccagca
    27001 tcagttcgga aacagtgctg atccccggaa tcagcggaat agtcccttcg gtagcagctt
    27061 tcagcagcgg ctcggtcaga cccgggctaa ttgcgaactg tgcacccgct tcagtgactt
    27121 ctgccagctg ctgtggattc agcaccgtac cggcacccac aatcgcttca ggcacttctt
    27181 tggcgatagc acggatagcg tcaactgcac actcggtacg cagagtcact tccagaacgc
    27241 gcaccccacc agcaaccaac gcttttgcca tcggcaccgc gtgttccagt ttttttacca
    27301 cgataaccgg tacaaccggg ccggtggtca ggattgattc tgcacttgtt ttccagtttt
    27361 tcatcagagt tttctctcgc ctgattacaa atttgtcgtc ttaaaaagtg atacaggttg
    27421 cgccctgttc ggcaccggac agtttttcac gcaaggcgct gaataattca cgtcctgttc
    27481 ccacgcgtga cgcgctcagg tcaggaatgt gtggttcgcg agcagccagt tccgcttcgt
    27541 ctaccagcag cgtcagttcg cctgtctgtc cattcacacg aatgatgtcc ccgtcgcgca
    27601 cttttgccag cagcccgcca tcgtaggctt ctggtgttac gtggatagct gacggcactt
    27661 tacctgaagc gccggagagt cgtccatcgg taactaacgc aattttgaaa caccggtcca
    27721 ataatacacc aagtggcggc atgagtttat gtaattctgg cattccgttc gcttttggcc
    27781 cctgatgacg gacaacaacg acacaatcgc ggtccagcaa acccgcttca aaggccggca
    27841 taacgtcatg ctggctttca aaaacaaccg ctggcgcttc aatcacctgg ttctcaaccg
    27901 gcacggcaga ggttttcata accgcacggc ccaggttacc gcttaacact tttgtcccac
    27961 catgatgaga gaaaggttgt tcgaaggaag cgatcacatt gctgtcgagt gatttttccg
    28021 ccccttcccg ccagtccagt tcaccattat tcagccatgg ttcaagggta taacgagaca
    28081 gaccaaagcc tgccaccgta ttgacatctt catgcagcag gcctgctttg agcagttcac
    28141 gcaccagaac cggtacgcca cctgccgcct ggaagtggtt aatatcggcc ggaccgttcg
    28201 ggtagagacg agccatcagc ggtacaacat cagaaaggtc agagaagtca tcccagttaa
    28261 tctgaatacc ggccgcgcgc gccatcgcca ccaggtgcat ggtgtggtta gtggaaccac
    28321 cggtcgccag cagtgcaacg ataccgttca ccaccacttt ctcatcgatc atcttaccga
    28381 tcggcatcca ttcattacca ttaccggtca tgcgtgtaac ctgacgcgca gcggcagcgg
    28441 tcaaagcatc gcgcagcgga gaatccggat gaacaaaaga agagcctggc aactgcatcc
    28501 ccataaactc caccaccatc tggttggtgt tggcagtacc gtagaaagta catgttcccg
    28561 gcgcatggta agacgcggct tctgactcca gtaaggccat gcggtccact ttaccttcgg
    28621 cataaagctg gcgaatacgc actttttctt tatttggcaa accgcttgcc atcggtccag
    28681 acggcacaaa caccgcaggc aaatgaccaa acgacagggc tgccatcgtc agacccggga
    28741 caatcttgtc gcacaccccg aggaacagag caccatcaaa catgttatgg gacagcccga
    28801 ccgccgcaga catcgctatc acttcgcggc ttagcagcga caattccatt ccatcctgcc
    28861 cctgggtgac accatcacac atcgccggaa caccacccgc aacctgacca accgcattcg
    28921 cttcatgcag ggctttacga atgatttctg gatagtgttc ataaggctgg tgagcggaga
    28981 gcatgtcgtt ataggaggtg atgatggcga tattgttacg caacatgctt ttcaaagagg
    29041 ctttgtcttc tggctggcag gcagcgaaac cgtgtgccag gttaccgcat gccaactgcg
    29101 aacgatgaac ggtcgacgtt ttcgcttgtt ctatccgggc gagataagca gagcgagtct
    29161 cgcgcgaacg ttcaatgatt cgatttgtta cgcgtaacaa ttgtggattc ataaaggctc
    29221 ctgaaattga gttgtcagag caggatgatt cacaacgcgt ttcattcaga ggatttatga
    29281 gtgaaacgcc tgtaaccgga gctcataggg caaaaacgtt tcagtcagtg taataaaaaa
    29341 agcctcgtgg gtgaatccgc acgaggcctg aaagtgtaaa aattgttcta caatctgtgc
    29401 aagatcatgt taccggtaaa ataaccataa aggataagcg cagatattac tcaaactcat
    29461 tccaggaacg accatcacgg gtaatcatcg ccaccgaggc aacgggtccc caggttccgg
    29521 cctgatacgg tttcggcgca tcattgtcca tcgcccacgc ctcagtaatg gagtctaccc
    29581 atttccaggc ttcttccact tcatcgcgac gtacaaacag tgcctgaata ccacgcatgg
    29641 tttccagcag caaacgttca taggcatccg ccagatgcgt ctgattaaag gtttctgaat
    29701 agctcagatc cagcttggtg atttgcaggt tatgtttgtg gtcaaggcca ggaactttat
    29761 tcagtacctg gatatccacg ccttcatcag gttgcagacg gatagtcagt ttattctgcg
    29821 gcagatcctg ccacgactct ttaaacagat tcagttcagg tgttttgaaa tagaccacga
    29881 cttcagaaca tttggtcggc agacgtttac cagtacgcag gtagaatggc acaccggccc
    29941 agcgccagtt atcaatgtcg acgcggatcg ccacgaaggt ttctgtattg ctgctcttgt
    30001 tcgcgccctc ttcttccaga tatcccggca cttttttgcc ctgggcgaag cccgcagtat
    30061 attgcccgcg tacggttttt tcgcgtacgt tggagcggtc gatgcggcgc agagacttca
    30121 gtactttcac tttttcatcg cgaatgctgt ctgcgctcag gtcagacggc ggagacatcg
    30181 caatcatgca aagaatttgc agcaggtggt tctggatcat gtcgcgcatc tgaccggctt
    30241 tatcaaaata gccccagcgc ccttcgatcc ccacttcttc tgccacggta atctcaacat
    30301 gatcaatggt gcgattgtcc cagttattca caaacaggga gttagcaaaa cgcagcgcca
    30361 acaggttcag caccgtttct ttaccaagat agtggtcgat acggtaaacc tggcactcct
    30421 cgaagtattc gccaacctga tcattgattt cctgcgaggt cgccagcgac gtccccagcg
    30481 gtttctccat gactacgcgt gccggtttag cattcagttt tgcctcgcca agccctttgc
    30541 aaattgcgcc aaaagtgctg ggcggcatgg caaagtagtt aatggtgata cgattttttt
    30601 gatccagcat cgcgccgaga cggttgaatg cagcagtgtc attgacatcg agattacaaa
    30661 aatccagacg tgcactcagg gtgtcccata aaccttcatc aatggtttct ttcatgaaag
    30721 tttcgagcgc ctcgcggaca actttggtat atgccgcttt atcccagtca gcacgcccta
    30781 cgccgataat ccgggtgtcc gggttgagct gaccggcttt ttccagttga tacagggaag
    30841 gcagcaattt acgacgcgca aggtcgcctt tcgcgccgaa aatgaccagg tcacaggcct
    30901 gggctgtttg cgttaccgcc atgtcattct ccttaagtta actaacccgg tacttaagcc
    30961 agggtatact tgtaattttc ttacggtgca ctgtactgct tttacgagct tgcgaaaact
    31021 gtaaacgctt atccacccgt gcgattacgg taaaagcgcg taaagtgcgg caaaactgat
    31081 aaaaaaatca tcgttttcca ttgggtaaaa atctgacact gatcatgtta tgaaaaaaaa
    31141 taacaacttt tttatctgct tttgtcatta acacagcaca caggcgtaat atctgacaaa
    31201 actgcatttc gatttctttc agtgcggaaa tcgtcattac ccgtgagtct ctttacatca
    31261 tgaatatgct ggaaaaaatc cagtctcagc tggaacattt gagcaaatcg gagcgcaaag
    31321 ttgccgaggt cattctggct tcgcccgata acgcgatcca ttcgagtatt gctgctctgg
    31381 cgctggaagc caatgttagc gaaccgacgg tgaatcgttt ctgtcgcagc atggacacgc
    31441 gcggttttcc tgattttaaa cttcatctgg cacagagtct ggcgaatggc actccctatg
    31501 ttaatcgcaa cgtcaatgaa gatgacagcg ttgaatcata cacagggaaa atatttgagt
    31561 ccgcaatggc aacgctcgat catgtccgtc attcactgga taaatctgcc atcaaccgcg
    31621 ccgtcgactt gctcactcag gcaaaaaaaa tcgccttttt cggattaggc tcttcagccg
    31681 ccgttgccca cgatgcgatg aataagttct ttcgttttaa tgttccggtg gtgtactccg
    31741 atgatatcgt gctgcaacgc atgagttgta tgaattgtag cgacggagac gtggtggtgc
    31801 tgatttctca cactggaaga acaaaaaatc tggtcgagct ggcgcagctg gcacgcgaaa
    31861 acgacgccat ggtgattgcc ctcacctctg cgggtacccc gctcgcccgg gaagcaacgc
    31921 tggcaattac cctcgacgta ccggaagata ctgacattta tatgcccatg gtttctcgac
    31981 ttgcacagct gaccgtgata gatgtgctgg cgacaggatt tactttgcga cgcggtgcaa
    32041 aattcagaga taacttgaag cgggtcaagg aagcgctgaa ggaatcgcgt tttgataagc
    32101 agttacttaa tttaagtgac gatcgctaaa agcgactgtc actgttctaa tcttatacga
    32161 catccgaatg agattaattt atcgccatcg cggcgttatt tcattcggat ttcatgttca
    32221 agcaacacct ggttgtttca gtcaacggag tattacatgt ccagaaggct tcgcagaaca
    32281 aaaatcgtta ccacgttagg cccagcaaca gatcgcgata ataatcttga aaaagttatc
    32341 gcggcgggtg ccaacgttgt acgtatgaac ttttctcacg gctcgcctga agatcacaaa
    32401 atgcgcgcgg ataaagttcg tgagattgcc gcaaaactgg ggcgtcatgt ggctattctg
    32461 ggtgacctcc aggggcccaa aatccgtgta tccaccttta aggaaggcaa agttttcctc
    32521 aatattgggg ataaattcct gctcgacgcc aacctgggta aaggtgaagg cgacaaagaa
    32581 aaagtcggta tcgactacaa aggcctgcct gctgacgtcg tgcctggtga catcctgctg
    32641 ctggacgatg gtcgcgtcca gttaaaagta ctggaagttc agggcatgaa agtgttcacc
    32701 gaagtcaccg tcggtggtcc cctctccaac aataaaggta tcaacaaact tggcggcggt
    32761 ttgtcggctg aagcgctgac cgaaaaagac aaagcagaca ttaagactgc ggcgttgatt
    32821 ggcgtagatt acctggctgt ctccttccca cgctgtggcg aagatctgaa ctatgcccgt
    32881 cgcctggcac gcgatgcagg atgtgatgcg aaaattgttg ccaaggttga acgtgcggaa
    32941 gccgtttgca gccaggatgc aatggatgac atcatcctcg cctctgacgt ggtaatggtt
    33001 gcacgtggcg acctcggtgt ggaaattggc gacccggaac tggtcggcat tcagaaagcg
    33061 ttgatccgtc gtgcgcgtca gctaaaccga gcggtaatca cggcgaccca gatgatggag
    33121 tcaatgatta ctaacccgat gccgacgcgt gcagaagtca tggacgtagc aaacgccgtt
    33181 ctggatggta ctgacgctgt gatgctgtct gcagaaactg ccgctgggca gtatccgtca
    33241 gaaactgttg cagccatggc gcgcgtttgc ctgggtgcgg aaaaaatccc gagcatcaac
    33301 gtttctaaac accgtctgga cgttcagttc gacaatgtgg aagaagctat tgccatgtca
    33361 gcaatgtacg cagctaacca cctgaaaggc gttacggcga tcattaccat gaccgaatcg
    33421 ggtcgtaccg cgctgatgac ctcccgtatc agctctggtc taccaatttt cgccatgtcg
    33481 cgccatgaac gtacgctgaa cctgactgct ctctatcgtg gcgttacgcc ggtgcacttt
    33541 gatagcgcta atgacggcgt agcagctgcc agcgaagcgg ttaatctgct gcgcgataaa
    33601 ggttacttga tgtctggtga cctggtgatt gtcacccagg gcgacgtgat gagtaccgtg
    33661 ggttctacta ataccacgcg tattttaacg gtagagtaag tacgttgccg gatgcggcga
    33721 aaacgccaca tccggcctac agttcaatga tagttcaaca gatttcgaat attctgaagc
    33781 aaacttgaac ttatcatcag gcgaaggcct ctcctcgcga gaggcttttt tatttgatgg
    33841 gataaagatc tttgcgctta tacggctgga tttcgcccgg tttgcgagtt ttcagcaatt
    33901 ttagtatcca ggtgtattgt tctggtcgcg gaccaacaaa aatctcgact tcttcattca
    33961 tccgccgcgc aatcgtatga tcatccgcct ctaacagatc atccatcggt gggcgcacct
    34021 gaatcgtcag acgatgcgtc ttgccatcat aaatcggaaa cagcggtaca acgcgcgcac
    34081 ggcacacttt catcaaacga ccaatcgcgg gcaacgtcgc tttataggtg gcaaagaaat
    34141 ccacaaattc gctgtgctct gggccatgat cctgatcggg taaataatat ccccagtacc
    34201 cctgacgtac cgactggatg aatggtttaa taccgtcatt tctcgcatgc agacgaccgc
    34261 caaagcgacg acgcaccgtg ttccagacat aatcaaaaac cgggttgccc tgattatgga
    34321 acatcgctgc cattttctgc ccttgcgagg ccatcagcat ggcaggaata tcgacggccc
    34381 aaccgtgcgg caccagaaag ataactttct cgttattacg ccgcatctct tcgatgatct
    34441 ccagcccttg ccagtcaact cgcggctgaa ttttctccgg cccgcgtatt gccaactcag
    34501 ccatcattgc catcgcttgc ggcgcggtgg caaacatctc atcaacaatc gcttcgcgtt
    34561 cagcttcact acgttctgga aagcagagcg acagattgat taacgcacga cggcgtgagc
    34621 tttttcccag tcgtccggca aaacgtccca gccgtgccag aatgggatca cggaactttg
    34681 gcggcgttaa agcgataccc gccatcgctg ctacgcccag ccatgctccc cagtagcgcg
    34741 ggtggcgaaa ggatttatca aactcaggaa tgtattcgct attatttttt ttcgtttcca
    34801 tgcttttcca gtttcggata aggcaaaaat caatctggtg atagtgtagc ggcgcaactt
    34861 gccccgcacc aaataaaaaa agccggtact gactgcgtac cggctgcgaa tggatgttaa
    34921 ttaatcaaac cgtagctgcg gcacaatctc tttggcctgc gccaggaatt cgcgacgatc
    34981 ggagccggtc agcccttcgg tacgcggcag ttttgccgtc agcgggttta cggcctgctg
    35041 gtttatccat acttcatagt gcagatgcgg cccggttgaa cgtccggtat taccggaaag
    35101 cgcgatacgg tcgccacgtt tcaccttctg tcccggtttc accagaatct tgcgcaagtg
    35161 catataacgc gtggtgtagc tgcgaccatg acgaatagcc acataataac ctgctgcgcc
    35221 actgcgtttg gcaaccacca cttcaccgtc acccactgaa agcactggcg taccctgcgg
    35281 catggcgaaa tcaacacctc tgtgtggtgc aacgcgaccg gtcaccggat tagtacgacg
    35341 cgggttaaag ttagacgaga tacggaactg tttcgccgtc gggaatcgca agaatccttt
    35401 cgccagacca gtaccgttac gatcgtagaa tttgccatct tcagcgcgga tagcgtaata
    35461 atctttacct tctgaacgca aacgtacgcc cagcagctgg ctttgctcac gtttaccatc
    35521 aagcatttct cgagacatta ataccgcaaa ttcatcgcct tttttcagtt tgcggaaatc
    35581 catttgccac tgcatggctt taatcactgc gctcacttcg gcgctggtta aaccggcgtt
    35641 tctggcgctg gcaacaaagc tccccccgac ggtacctttc agcagattgt taacccactc
    35701 tccttgctgc atttcgctgg tcattttaaa accgttagca gcagtacggt cataggttcg
    35761 ggtttcacga cgagacactt cccaggtgag gcgctgcagt tcgccgtccg cggttaatgt
    35821 ccaggagagt tgttgaccga ttttcaggtt acgcaattct ttgtcggccg cagccagttg
    35881 ggtgatatca cccatatcaa taccatactg attgagaatg ctgcttagcg tatcgccagt
    35941 ggaaacaaca tattcatgca cgcccgcttc accggcaatt ttgtcatcca gttcgtcctg
    36001 gggaatggct tcatcttctt gtgcagcttg atcaatcggt tcactggctt caggtaagag
    36061 cgaacgaatt tcgttctgtt ccagctcaat ggttttgaca attggcgtgg catcgcggtg
    36121 ataaacatag ggccgccaga cagcgacggc cagagtaaga acggtgagcg accccaacat
    36181 aacgcggtgt ggtcgcggta aattattaaa cgccagggcg acagagcggg ctatctgttg
    36241 cacgtaatca cttcctcatt aatctccttt caggcagctc gcatactggt tggctaattg
    36301 actcaggaat tctgaatagc ttgttttacc cagtttgata ttcgtcccca ggggatccaa
    36361 cgttcccata cgaacggatg tccctcgtgc gacgctctca acgaccgctg gcctgaactg
    36421 tggctcagca aaaacgcagg ttgctttttg ctcaaccaac tgtgttctta tttcatgtaa
    36481 acgctgcgcg ccaggttgaa tctcagggtt aacggtaaaa tgaccaagcg gtgtcagtcc
    36541 gaactgtttt tcgaaatagc cgtaagcatc gtgaaaaacg aaataacctt tccccttgag
    36601 cggcgcgagc tcgttaccaa cctgcgtttc ggttgaagct aattgtgcct caaaatcctt
    36661 caggttggcg tcaagtttgg ctcgactttg cggcataagt tccactaatt ttccatggat
    36721 tgcaaccgct gtagcccgcg ctatctctgg ggaaagccaa agatgcatgt tgaaatcgcc
    36781 gtgatggtga tcttcgtcac ttttttccgc gtggtcgtga tcatcatcat cgccgtgaat
    36841 acttttcatc agtagcggtt tcacatcttc tagctgcgca atcgttacct gcttcgcttc
    36901 tggtaatttg cttaccggtt tttgcataaa cgcttccatc tccgggccaa cccaaacgac
    36961 taagtccgcg ttctgtaagc gttttacatc cgatggacgc agtgaataat catgttctga
    37021 agcgccgtca ggaagtaaaa cctccgtttc tgttacccca tcagcaatgg cagaagcgat
    37081 gaacccaacg ggtttaagcg aagcgacaac ggcggcatct gcggcctgtg ttgcaccgcc
    37141 ccagagagcg gcggataatg ctgcgaaaag aagcgttttt ttatgtaaca taatgcgacc
    37201 aatcatcgta atgaatatga gaagtgtgat attataacat ttcatgacta ctgcaagact
    37261 aaaattaaca tgacaagtct ggtttccctg gaaaatgtct cggtttcttt tggccaacgc
    37321 cgcgtcctct ctgatgtgtc gctggaactt aaacctggaa aaattttgac tttacttggg
    37381 ccaaatggcg caggtaagtc gacactggta cgggtagtgc tcgggctggt aacacccgat
    37441 gaaggggtta tcaagcgcaa cggaaaactg cgcatcggct atgtaccgca gaagctgtat
    37501 ctcgacacca cgttgccact gaccgtaaac cgttttttac gcttacgccc tggtacacat
    37561 aaagaagata ttttgcctgc actaaaacgt gtccaggccg ggcacctgat taacgcaccg
    37621 atgcaaaagc tctcgggtgg cgaaacgcag cgtgtactgt tagcgcgagc attgttaaat
    37681 cgcccgcaat tattagtgct ggatgaaccc actcaaggcg tggatgtaaa tggccaggtg
    37741 gcgttatatg accttattga ccaactgcgt cgtgaactgg attgtggcgt tttaatggtc
    37801 tctcacgatc tgcatctggt aatggcaaaa accgatgaag tgctgtgcct gaatcaccac
    37861 atttgttgtt ccggcacacc ggaagttgtt tccctgcatc cggagtttat ttcaatgttt
    37921 ggtcctcgtg gtgctgaaca actgggtatc tatcgccatc atcataatca tcgtcacgat
    37981 ttacagggac gaattgtttt gcgtcgggga aatgatcgct catgattgaa ttattatttc
    38041 ccggttggtt agccgggatc atgctcgcct gtgccgcggg tccgcttggt tcgtttgtag
    38101 tctggcgtcg tatgtcttat ttcggtgata cgctggctca tgcctcatta cttggtgtcg
    38161 cgtttggttt gttgctggac gtgaatccat tctatgcggt gattgccgtt acgctgctgc
    38221 tggcgggcgg tctagtatgg ctggagaagc gtccacagct ggcgatcgac acgttattag
    38281 ggattatggc gcacagtgcc ctgtcgctgg gcctggtggt cgttagtctg atgtctaaca
    38341 ttcgtgttga tttgatggct tacctgttcg gtgatttact ggcagtgacg ccagaagatc
    38401 tcatctctat tgcgattggc gtggtcatcg tggtggcgat tttgttctgg caatggcgca
    38461 atttgctgtc gatgacgatt agcccggatc tggcgtttgt tgatggtgtg aaattacagc
    38521 gcgtgaaatt gttgttgatg ctggtgacgg cattgacgat tggtgtagcg atgaaatttg
    38581 tcggcgcgtt gattattact tcgctgctga ttattcctgc agctactgca cgtcgctttg
    38641 cccgcacgcc ggaacagatg gctggtgtcg ctgttttggt ggggatggtg gcagtgactg
    38701 gcggtttaac cttttccgca ttttacgata cgccggcggg tccgtcggta gtactatgtg
    38761 ctgcactatt gtttattatc agtatgatga aaaaacaggc cagttaattt aaacctaaaa
    38821 tgtcggatgc ggcgcgagcg ccttttccgc cctacggttc gctatctctg gtatatgcct
    38881 gataagacgc gaatagcgtc gcatcaggca cactgccaat gtcggatgcg gtgcgagcga
    38941 ccaatccgac ttacggcatt tctggcggcg ttatgccaaa gtgattccac gcccgcgtcg
    39001 tcgccatacg cccgcgcggt gtacgctgca aaaagccttg ctgaatcaaa taaggttcca
    39061 gcacatcctc aatggtttca cgttcttcgc caatagccgc cgccaggtta tccagaccta
    39121 ccggcccacc aaagaactta tcgattaccg ccagcaacaa tttgcggtcc atataatcga
    39181 aaccttcagc atcgacattc aacatatcca gcgcctgagc agcgatatct gccgagatgg
    39241 tgccatcgtg cttcacttcg gcgaaatcac gcactcgacg cagcagacgg ttggcaatgc
    39301 gcggcgtacc gcgagcgcga cgagcaactt ccagcgcgcc gtcatcactc atctcaagcc
    39361 ccataaagcg tgcgctgcga ctgacgatat attgcagatc cggcacctga taaaactcca
    39421 gacgttgcac aataccaaaa cggtcgcgca acggtgatgt cagcgaacct gcgcgcgtgg
    39481 ttgcaccaat cagggtaaac ggcggcaaat caattttaat ggagcgtgcc gccggacctt
    39541 caccaatcat gatatccagt tggtagtctt ccattgccgg gtacagcact tcttcaacaa
    39601 ctggcgataa acggtggatc tcatcaataa acagcacgtc atgcggttca aggttagtga
    39661 gcatcgcagc caaatcgccc gccttttcca gcaccggacc agaagtcgtg cgtaaattaa
    39721 cgcccatttc attggcgaca atgttggcaa gcgtagtttt acccaacccc ggaggaccaa
    39781 aaatcaacaa atgatcgagg gcatcgccgc gcagtttcgc tgctttgatg aaaatctcca
    39841 tctgtgaacg aacctgcggc tgaccaacat actcttccag taatttaggg cgaatggcgc
    39901 gatctgctac atcttccggc aaagtggtac cggcagaaat cagacggtct gcttcaatca
    39961 tcctttacct cataacgcgg cgcgtagggc ttcgcgaatt aatgtttcac tgctggcgtc
    40021 agggcgagcg attttgctca ccatgcggct tgcttcttgt ggtttatagc ccagcgccac
    40081 cagcgcggca accgcttctt gttcagcatc gtcggtcgcc gggctggcag gagacgtgag
    40141 taccaggtcg gcggctggcg taaagagatc gccatgcaaa cctttaaatc ggtctttcat
    40201 ttcaacaatc aagcgttcgg cggttttttt gccaatacct ggcagtttca ccagtgcccc
    40261 cacttcttca cgctcaacgg cattaacaaa ctgctgcgct gacattccgg agaggatcgc
    40321 cagcgccaac ttcgggccga cgccgttggt tttgatcaac tctttgaaca atgtgcgctc
    40381 ttgtttattg ttaaaaccgt acagcagttg cgcgtcttca cgcaccacaa agtgggtgaa
    40441 aacgatcgct tcctgacccg cttcagggag ttcataaaaa caggtcatcg gcatatgcac
    40501 ttcatagcct acgccgccca cttcaattaa caccagcggg ggttgttttt caatgatgat
    40561 gcctctgagt ctgcctatca catgacgctc ctgcgtaatg aatcaaagat aatgctgtat
    40621 gataaaaaaa tgctggatag atatccagcg aaggatgaag aaaacttgcg aggtgtctcg
    40681 atgaactgaa aaatggcaca gtataattta ttctacaggt tatattggaa gcaaatattt
    40741 taatattaca tattcagtga agaaatgcgt aataaaaata tacattgcgc ctcctgaaaa
    40801 aataa
//
LOCUS       DAZUCE010000016        38173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZUCE010000016 DAZUCE010000000
VERSION     DAZUCE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.016"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..792
                     /gene="ytfI"
                     /locus_tag="ACHY03_000786"
     CDS             <1..792
                     /gene="ytfI"
                     /locus_tag="ACHY03_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFS3904055.1"
                     /translation="LLTLREFFRCATQIDKSSYQVLSSKNETVNAMDKFLISFSLKDN
                     GAEYTMTLRGSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIP
                     KRIKGYIHQAIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDN
                     FAIVIESLGFALKIYGGDESFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAI
                     VSANLAADSDITKEKICEKYKSTIEAFKKEQLALPVLVRRKNSET"
     gene            complement(855..1409)
                     /gene="ytfJ"
                     /locus_tag="ACHY03_000787"
     CDS             complement(855..1409)
                     /gene="ytfJ"
                     /locus_tag="ACHY03_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFS3904056.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            1734..1940
                     /gene="ytfK"
                     /locus_tag="ACHY03_000788"
     CDS             1734..1940
                     /gene="ytfK"
                     /locus_tag="ACHY03_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFS3904057.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2019..3362)
                     /gene="paeA"
                     /locus_tag="ACHY03_000789"
     CDS             complement(2019..3362)
                     /gene="paeA"
                     /locus_tag="ACHY03_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFS3904058.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(3685..4323)
                     /gene="msrA"
                     /locus_tag="ACHY03_000790"
     CDS             complement(3685..4323)
                     /gene="msrA"
                     /locus_tag="ACHY03_000790"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFS3904059.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4529..6262
                     /gene="tamA"
                     /locus_tag="ACHY03_000791"
     CDS             4529..6262
                     /gene="tamA"
                     /locus_tag="ACHY03_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFS3904060.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6259..10038
                     /gene="tamB"
                     /locus_tag="ACHY03_000792"
     CDS             6259..10038
                     /gene="tamB"
                     /locus_tag="ACHY03_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFS3904061.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10041..10382
                     /gene="ytfP"
                     /locus_tag="ACHY03_000793"
     CDS             10041..10382
                     /gene="ytfP"
                     /locus_tag="ACHY03_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFS3904062.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10594..10845
                     /gene="chpS"
                     /locus_tag="ACHY03_000794"
     CDS             10594..10845
                     /gene="chpS"
                     /locus_tag="ACHY03_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFS3904063.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10839..11189
                     /gene="chpB"
                     /locus_tag="ACHY03_000795"
     CDS             10839..11189
                     /gene="chpB"
                     /locus_tag="ACHY03_000795"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFS3904064.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(11269..11799)
                     /gene="ppa"
                     /locus_tag="ACHY03_000796"
     CDS             complement(11269..11799)
                     /gene="ppa"
                     /locus_tag="ACHY03_000796"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFS3904065.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            12109..13065
                     /gene="ytfQ"
                     /locus_tag="ACHY03_000797"
     CDS             12109..13065
                     /gene="ytfQ"
                     /locus_tag="ACHY03_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFS3904066.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13205..14707
                     /gene="ytfR"
                     /locus_tag="ACHY03_000798"
     CDS             13205..14707
                     /gene="ytfR"
                     /locus_tag="ACHY03_000798"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFS3904067.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14721..15743
                     /gene="ytfT"
                     /locus_tag="ACHY03_000799"
     CDS             14721..15743
                     /gene="ytfT"
                     /locus_tag="ACHY03_000799"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFS3904068.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALSTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15730..16725
                     /gene="yjfF"
                     /locus_tag="ACHY03_000800"
     CDS             15730..16725
                     /gene="yjfF"
                     /locus_tag="ACHY03_000800"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFS3904069.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(16758..17756)
                     /gene="fbp"
                     /locus_tag="ACHY03_000801"
     CDS             complement(16758..17756)
                     /gene="fbp"
                     /locus_tag="ACHY03_000801"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFS3904070.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            17932..19305
                     /gene="mpl"
                     /locus_tag="ACHY03_000802"
     CDS             17932..19305
                     /gene="mpl"
                     /locus_tag="ACHY03_000802"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFS3904071.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19461..20012)
                     /gene="yjgA"
                     /locus_tag="ACHY03_000803"
     CDS             complement(19461..20012)
                     /gene="yjgA"
                     /locus_tag="ACHY03_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFS3904072.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20106..21458
                     /gene="pmbA"
                     /locus_tag="ACHY03_000804"
     CDS             20106..21458
                     /gene="pmbA"
                     /locus_tag="ACHY03_000804"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFS3904073.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21642..22028
                     /gene="cybC"
                     /locus_tag="ACHY03_000805"
     CDS             21642..22028
                     /gene="cybC"
                     /locus_tag="ACHY03_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFS3904074.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAVALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(22073..22537)
                     /gene="nrdG"
                     /locus_tag="ACHY03_000806"
     CDS             complement(22073..22537)
                     /gene="nrdG"
                     /locus_tag="ACHY03_000806"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFS3904075.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22727..24865)
                     /gene="nrdD"
                     /locus_tag="ACHY03_000807"
     CDS             complement(22727..24865)
                     /gene="nrdD"
                     /locus_tag="ACHY03_000807"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFS3904076.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25259..26914)
                     /gene="treC"
                     /locus_tag="ACHY03_000808"
     CDS             complement(25259..26914)
                     /gene="treC"
                     /locus_tag="ACHY03_000808"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFS3904077.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKKLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IHPWQPEQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(26964..28385)
                     /gene="treB"
                     /locus_tag="ACHY03_000809"
     CDS             complement(26964..28385)
                     /gene="treB"
                     /locus_tag="ACHY03_000809"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFS3904078.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28504..29451)
                     /gene="treR"
                     /locus_tag="ACHY03_000810"
     CDS             complement(28504..29451)
                     /gene="treR"
                     /locus_tag="ACHY03_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFS3904079.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            29636..29689
                     /gene="mgtL"
                     /locus_tag="ACHY03_000811"
     CDS             29636..29689
                     /gene="mgtL"
                     /locus_tag="ACHY03_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFS3904080.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29830..32526
                     /gene="mgtA"
                     /locus_tag="ACHY03_000812"
     CDS             29830..32526
                     /gene="mgtA"
                     /locus_tag="ACHY03_000812"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFS3904081.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32732..33118)
                     /gene="ridA"
                     /locus_tag="ACHY03_000813"
     CDS             complement(32732..33118)
                     /gene="ridA"
                     /locus_tag="ACHY03_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFS3904082.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33191..33652)
                     /gene="pyrI"
                     /locus_tag="ACHY03_000814"
     CDS             complement(33191..33652)
                     /gene="pyrI"
                     /locus_tag="ACHY03_000814"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFS3904083.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRAKDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33665..34600)
                     /gene="pyrB"
                     /locus_tag="ACHY03_000815"
     CDS             complement(33665..34600)
                     /gene="pyrB"
                     /locus_tag="ACHY03_000815"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFS3904084.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34604..34738)
                     /gene="pyrL"
                     /locus_tag="ACHY03_000816"
     CDS             complement(34604..34738)
                     /gene="pyrL"
                     /locus_tag="ACHY03_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFS3904085.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35019..35414)
                     /gene="yjgH"
                     /locus_tag="ACHY03_000817"
     CDS             complement(35019..35414)
                     /gene="yjgH"
                     /locus_tag="ACHY03_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HFS3904086.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(35545..36258)
                     /gene="bdcA"
                     /locus_tag="ACHY03_000818"
     CDS             complement(35545..36258)
                     /gene="bdcA"
                     /locus_tag="ACHY03_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3904087.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGSLDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36329..36922
                     /gene="bdcR"
                     /locus_tag="ACHY03_000819"
     CDS             36329..36922
                     /gene="bdcR"
                     /locus_tag="ACHY03_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFS3904088.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            37067..37519
                     /gene="tabA"
                     /locus_tag="ACHY03_000820"
     CDS             37067..37519
                     /gene="tabA"
                     /locus_tag="ACHY03_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFS3904089.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            37642..>38173
                     /gene="yjgL"
                     /locus_tag="ACHY03_000821"
     CDS             37642..>38173
                     /gene="yjgL"
                     /locus_tag="ACHY03_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjgL"
                     /protein_id="HFS3904090.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQRE"
BASE COUNT         9246 a         9441 c        10244 g         9242 t
ORIGIN      
        1 ctgttaactt tgagggagtt ctttcgttgc gcaactcaga ttgataaaag tagttatcag
       61 gtgctttcat caaaaaatga aactgttaac gccatggata agtttcttat cagtttcagc
      121 cttaaagata acggtgccga atatacaatg acattgcgag gcagtggatt tgaatacgaa
      181 gaaataccca taacaataaa tgaatataac tcattcatgg atttcaaaaa ccgcgagttt
      241 ccgttagaac aaaacagaag attgtatgcc tgggatattt tgcagaaaaa acagtctgac
      301 ataccgaaaa ggattaaagg ttatattcac caggcgattg gtgacgtgtc tttaggttat
      361 gccttattgg aggatatagt aagcaaatta aaacgaggta aatttgaact ccaaatacca
      421 ggaggtggaa ttaaagaatg cgatggatgg tatatttacg aaaaaataat tgatgataat
      481 tttgccatag tcatagaatc tttgggcttt gcgcttaaaa tctatggagg ggatgaaagt
      541 tttcgtaatg gttcctctgt tgttttggag gatgaagatt actctcttat ttataatttt
      601 ctggttaatg caggttgcca acaagtcgaa ttagccgaac aagtggatgc gatagtaagt
      661 gcgaatctgg ccgcagatag tgatattacc aaagaaaaga tttgcgagaa atataaatcg
      721 accattgagg cgtttaaaaa agagcaacta gcgttaccag tattagttcg gcgtaagaac
      781 tcagagactt aattccccta tcccatagat aacgataggg gaaaattatt tttccagaat
      841 ttatgttatt gccattattt gagcaacttt tgcagcaggt ccatcacttg ctgcacctct
      901 tccggtgtga gcgccccgtc tttggcccat tgcacgcgac cgtctttatc cagcaccact
      961 acagcagaac tttcttcatc cagctgccag gcaccgcgcg cgacgccatt gctatcgaca
     1021 ataaactgcg accagggata aagcttttta ttactctcca gactgctgcg cacaaacatg
     1081 ccggaacccg gaatcgcgtc gtcggtgtta acaatggtgg tggtctggta acgatcgtgc
     1141 ggtaacttcg ctgatttaat cgcttcaatc agcgtcgcgt ttttctcttt tgcagaggtg
     1201 cgaccagcaa tatgttgcag tactcgcact tttcccacta actgcgcgct gttccaggtt
     1261 ttgtagctaa actgatcttt atcaagcacc aactcgcccc gatcggtaat gccaatcggc
     1321 ggcactcgct gaccggtttc gaactgatgt gcggaagcca tcatcggcaa cagcaggcag
     1381 gtgagtgcca gaatcttgcg tagggtcatg gtgtttcctt cttatgatat gcaggtgatc
     1441 cgaccacttg ggtctgagtt ttaatcatat gtgctattta tcgaatttcc cgcaagtgtg
     1501 atgccagttt gcggtcaagc gcacaaatca tatgaaaaat gaatgcttat actgaagacc
     1561 gcgcttcggt aaaaagataa ttctgaataa ttgtaacctt taggtaaaaa aagttatacg
     1621 cggtggaaac attgcccgga tagtctatag tcactaagca ttaaaatttg cgcctcataa
     1681 tagttgggcc gattgtggca ccgcacaggc gtaatactca gcaggagata acaatgaaaa
     1741 ttttccaacg gtacaaccca cttcaggtgg cgaagtacgt aaagatcctg ttccgtggac
     1801 ggttatacat caaggacgtt ggcgcttttg aatttgataa gggtaagatt cttatcccaa
     1861 aagtgaagga taagctgcat ttgtcagtga tgtccgaagt taaccgtcag gttatgcgtc
     1921 tgcaaacaga gatggcttaa ccaaagtgct atgcagtaaa aagtgctatg cagtaataag
     1981 acggctcctg attcaggagc cgttgatgtt tctgggggtt acgcgacgct ttcttcttta
     2041 tctttagcgt caggcagttt tggcgaaagg gcggtggcct tgctgtcgat ccgggtcacc
     2101 agcagctggt cgatgcggta gttatcgata tccaccactt caaatttgta gccggcgaat
     2161 ttcaccgaat cggtgcgttt cgggatctta cgcagcataa acatcataaa gccgccgatg
     2221 gtttcgtagt tgcccgactg cgggaattcg tcaatatcca gcacgcgcat gacgtcgtca
     2281 atcggggtac cgccgtcaat cagccatgag ttctcatcac gggcgacaat ctgctcttcc
     2341 agcccctgac cgaccagatc gcccatcagc gtggtcatca cgtcgttgag ggtgatgatc
     2401 cccaccacca gcgcgtactc gttcatgatc accgcgaagt cttcacctgc ggttttaaaa
     2461 ctttccaacg cctctgaaag ggttaacgta tccggcacaa tcagcgtgtt gcgaatttgt
     2521 acgccgctgt tcagtgccag gctttggtta gccagcacgc ggttcagcag gtctttagaa
     2581 tcgacataac cgatgatgtg gtcaatatct tcattacaga cgaggaactt agagtgcgga
     2641 tgttccgcca ccttattctt caggctttgc tcatcttcgt ggagatcaaa ccaaatcacg
     2701 ttttcacgcg gtgtcattga cgacggaacg gtacgggatt ccagctcaaa gacgttttca
     2761 atcagctcgt gttcctgttt acgtaacacg cccgccagcg caccggcttc cactaccgcg
     2821 tagatgtcat cagaagtgat gtcatcttta cgtaccattg gcagtttgaa aatacggaag
     2881 atcatgttcg ccaggccgtt gaagaaccac accagcgggg tgcaaacgta caggcagaag
     2941 cgcatcgggt tgatgatacg caaagccacc gcttctggcg caatcatacc gatgcgtttc
     3001 ggggttaaat ccgcaaacag gataaacatg ccagtcacta acgagaaaga gagaataaag
     3061 ctcagttgct cagagagctc tgccgacata tagcgggaga acaggctgtg aaaagctgga
     3121 gaaaatgccg catcaccgac gataccgccg agaatcgcca ctgcgttcag accgatttgg
     3181 accacggtaa agaacatgcc gggattttcc tgcatattca gaacgcgttg ggcatttata
     3241 ttgccttcat cagccagcag tttaagtttg attttgcgtg aggcggcaag cgagatctcg
     3301 gacatcgaga agaacgcact tacagcgatc aagcagagta tgactaaaat actgtttaac
     3361 atatcttatc cgacctttca ggccagatcc tcggaagggg aagttgatta atttgtgtga
     3421 aatacacatt gaaagccgat tcgaagaaag tgaaccggca ttttcagtgg ctagtatagc
     3481 gtaaggtact gtaaagtcgc cagagggtta aaattcagac tgctgataaa tgcaatactg
     3541 cctgatgcgc tatgccgatc aggcctacgc agtccttgca atatattgaa tttggacgat
     3601 tttgtaggcc tggtaaggcg tttacgccgc atccggcata aataaagcgc actttatcaa
     3661 caatctacat ttgtacccgt aacgctatgc ttccggtggc agacagacgc caattccgcc
     3721 aattccacag taaccatacg ggtttttatg cagatattgc tggtggtcat cttcggcata
     3781 ataaaacggt gtggcgttag cgatttccgt ggtgatgtga cgatcgtcat cggcggcaag
     3841 catcgccgcc tgaaaacgtt ccagactggc gcgagctgcg gcatcctgtt ctggggtcag
     3901 cggataaatc gctgaacgat actgcgtgcc gtggtcattg ccctgacgca ttccctgggc
     3961 gggatcgtga ttctcccaaa atacctgtag caactgctca tagctgatga cggaaggatc
     4021 gtaaacaatg cgtaccgctt cggcatgacc cgtatcaccg gagcacactt cccgataagt
     4081 cggatttggc gtatagccgc cggtatagcc tgcggcggtg ctgtaaacgc cgggtaactg
     4141 ccagaacaga cgctccacac cccagaaaca acccatcgca aaaatggcaa tctccattcc
     4201 gtcaggtaca ttggtcattg agtgaccgtt gaccgcatgc agcgtggcta cgggcatcgg
     4261 ggtgttacgt ccaggcaggg catcggcggg ggaaaccaga tgctttttat caaataaact
     4321 catggtgtcg ctctcccgaa aatcggtcat tggggttaag gttgtaacaa gaggcgtatt
     4381 tgcacacaat aaccaccgtg aataggtcta aagtaaaaca taagaaatat ttggggttta
     4441 gtctgctttt taatccatat tactggattt ttgttaagcc gtttaacggc gttccagggg
     4501 caggaaaaaa ggatattcag gagaaaatgt gcgctatatc cgacagttat gctgtgtaag
     4561 cttactctgc ttaagcggat ctgccgtcgc cgcgaacgtc cgtctacagg tcgaggggtt
     4621 atcgggacag ctggaaaaga acgttcgtgc gcagctttct acgattgaaa gtgatgaagt
     4681 gacgccagac cgtcgctttc gcgcacgcgt cgatgatgct atccgcgaag gtctgaaagc
     4741 gctgggttat taccagccga ccattgaatt tgatctccgt ccaccgccaa agaaagggcg
     4801 gcaggtattg atcgccaaag tcacgccagg cgtgccggtg ttaattggcg gcaccgatgt
     4861 ggtattgcgc ggcggcgcgc ggaccgataa agactatttg aaattgctcg atactcgccc
     4921 ggctattggc acggtactga accagggcga ttatgaaaat ttcaaaaagt ccttaaccag
     4981 cattgcgttg cgtaaaggtt atttcgatag cgaatttacc aaagcgcagc tgggcattgc
     5041 gctcggcctg cataaagcct tctgggatat tgattataac agtggcgaac gttaccgctt
     5101 tgggcatgtg acctttgaag gatcacaaat ccgcgatgaa tacctgcaaa atctggtgcc
     5161 gtttaaagag ggcgatgagt acgaatcgaa agatctggca gaactgaacc gccgactttc
     5221 tgctaccggc tggtttaact cggtggtggt ggctccacaa tttgataaag cgcgcgaaac
     5281 gaaagtatta ccattgacgg gcgtggtttc gccgcgaaca gaaaacacca tcgaaaccgg
     5341 ggtcggttac tctacggacg tgggaccgcg cgtgaaagcg acgtggaaaa agccgtggat
     5401 gaactcttat ggtcacagtc tgaccaccag taccagtatt tccgcgccgg aacagatcct
     5461 cgacttcagc tataaaatgc cgctgctgaa gaatccactg gaacaatatt atttggtgca
     5521 gggcggtttt aagcgcactg acctgaacga taccgagtct gactccacta cgctggtggc
     5581 ttctcgctac tgggatctct ccagcggctg gcagcgtgcc attaacctgc gctggagtct
     5641 cgaccacttt actcagggtg aaattaccaa caccacgatg ctgttttatc ctggggtgat
     5701 gattagccgc acgcgttctc gtggtggcct gatgccaacc tggggcgact cgcaacgcta
     5761 ctctatcgac tactccaaca cggcctgggg ttcagatgtc gatttctccg ttttccaggc
     5821 gcagaacgtc tggatccgca cactgtacga tcgccatcgt tttgttacac gcggcacgct
     5881 gggctggatt gaaaccggtg atttcgacaa agtaccgccg gatctgcgtt tcttcgccgg
     5941 gggcgatcgc agtattcgcg gctacaaata caaatctatc gccccgaaat acgccaacgg
     6001 tgacctgaaa ggggcatcga agttgataac cggatcgctg gagtaccagt acaacgtgac
     6061 cggaaaatgg tggggcgcgg tgtttgtcga tagtggcgaa gcggtaagcg atattcgccg
     6121 cagcgacttt aaaaccggta ccggggtcgg cgtgcgctgg gaatcgccgg tcgggccaat
     6181 caaactcgat tttgccgtac cggtcgcgga taaagacgaa cacgggttac agttttacat
     6241 cggtctgggg ccagaattat gagtttatgg aaaaaaatca gcctcggcgt ggttatcgtt
     6301 atcttactgt tgctgggatc ggtggcgttt ctggtgggca ccaccagcgg cctgcatctg
     6361 gtatttaaag cggcggatcg ctgggtgcca ggactggata ttggcaaggt caccggcggc
     6421 tggcgcgatc tcaccttgtc tgacgttcgt tatgagcagc caggcgtggc ggtaaaagca
     6481 ggcaatctgc atctggcggt cgggcttgag tgcctgtgga acagtagcgt ttgtattaat
     6541 gacctggcgc tgaaagacat tcaggtcaac atcgacagta aaaaaatgcc tccttctgaa
     6601 caggttgaag aagaggaaga tagcggtccg ctggatctct ccacgccgta tcccatcacc
     6661 ctgacacggg tggcactgga caacgtcaac atcaagattg atgacaccac ggtatcggtg
     6721 atggacttca cctccggcct gaactggcag gagaaaaccc tgaccctgaa accgacgtcg
     6781 ctgaaaggcc tgctgattgc tctgccgaaa gtggcggaag tggcgcagga agaagtggtc
     6841 gaaccgaaaa ttgaaaatcc gcagccggag gaaaagccgc tcggcgaaac gctcaaagat
     6901 ctcttttctc gcccggtatt gccggaaatg accgacgtgc atttgccgct taacctgaac
     6961 attgaagagt ttaagggcga gcagctgcgc gtgacgggcg acacggacat caccgtgagc
     7021 accatgctgc tgaaagtgag cagcattgac ggcaatacta aactggacgc cctggatatc
     7081 gattccaatc aagggatcgt caacgccagc ggcacggcgc agctgtcaga caactggccg
     7141 gtggatatca ccctcaacag cacactgaac gtggagccgt tgaaaggtga aaaggtgaag
     7201 ctgaaagtgg gcggcgcgct gcgcgaacag ctggagatag gcgttaacct ttccggtccg
     7261 gtggatatgg atttacgcgc ccagacgcga ctggcggaag ccggattgcc gctcaacgtg
     7321 gaagtgaaca gcaaacagct ttactggccg ttcactggtg agaagcagta tcaggcggat
     7381 gatctgaaac tgaaacttac cggcaaaatg accgattaca cgctctctat gcgtacggca
     7441 gtgaagggac aggagatccc gcccgcgact attaccctcg acgccaaagg taatgaacag
     7501 caggtcaatc tcgacaaact caccgtcgcg gcgctggaag ggaaaactga actcaaggcg
     7561 ttgctcgact ggcaacaggc cattagttgg cgcggtgagc taacgcttaa tggcattaac
     7621 accgccaaag agttcccgga gtggccgtcg aaactcaatg gcttgattaa aacccgcggt
     7681 agcctgtacg gcggcacctg gcagatggag gtgccagaac tgaagctgac cggtaacgtc
     7741 aaacagaaca aagtgaacgt tgacggcacg ctgaaaggca acagttatat gcagtggatg
     7801 atcccagggc ttcatctgga actggggcca aacagtgccg aagtgaaagg cgagctgggg
     7861 gtaaaagatc tcaatcttga tgccaccatc aacgcgccgg ggctggataa cgcgctgccg
     7921 gggcttggcg gtacagcgaa agggctggtg aaagtacgcg gcacggtgga agcgccacaa
     7981 ctactggcag atatcaccgc gcgcggcctg cgctggcagg aactttccgt ggcgcaggtt
     8041 cgcgtggaag gcgacattaa atccaccgat cagatcgccg ggaaactcga cgtacgcgtt
     8101 gagcaaattt cgcagccgga tgtaaatatc aacctcgtca ccctgaatgc caaaggcagc
     8161 gaaaagcagc acgagctaca gttgcggatt cagggcgagc ctgtctccgg gcagcttaat
     8221 ctggcaggaa gttttgatcg caaagaagaa cgctggaagg gaactcttag caatacccgc
     8281 ttccagacgc cggtcggccc gtggtcgctg acccgcgata ttgcgctgga ttaccgcaat
     8341 aaggagcaaa aaatcagcat cgggccacac tgctggctta acccgaatgc ggaattgtgt
     8401 gtgccgcaaa ctatcgatgc gggggccgaa gggcgtgcgg tggtgaatct caaccgcttc
     8461 gacctcgcca tgctgaaacc gtttatgcca gaaaccactc aggccagcgg tatcttcacg
     8521 ggtaaagcgg acgttgcctg ggacaccacc aaagaggggc tgccgcaggg cagtatcacc
     8581 ctttcggggc gtaacgtgca ggtaacgcaa accgtcaacg atgcggcgct gcccgtggcg
     8641 tttcagacgc tgaatctgac ggcggaattg cgtaacaacc gtgccgaatt gggctggacc
     8701 atccgcctga ccaataacgg ccagtttgat ggacaggtgc aggtgaccga tccgcaaggc
     8761 cgccgtaatc ttggtggcaa cgtcaatatc cgtaacttca accttgcgat gataaacccc
     8821 atctttaccc gtggggaaaa agcagcgggg atggtgagtg ccaacttgcg tctgggtggt
     8881 gatgtgcaaa gcccgcagtt gtttggtcaa cttcaggtta ctggtgtgga tatcgacggc
     8941 aactttatgc cgtttgatat gcaaccgagc cagcttgcgg tcaactttaa cggtatgcgc
     9001 tcgacgcttg ccggtacagt acggacccag cagggggaaa tctacctgaa cggtgatgcc
     9061 gactggagcc aaattgaaaa ctggcgggcg cgggtaacgg cgaaaggcag taaagtgcgg
     9121 atcaccgtgc cgccgatggt acgaatggat gtatcgccag atgttgtatt cgaggctaca
     9181 ccaaacctgt ttaccctcga tggtcgcgta gatgtcccgt gggcgcgcat cgtggtgcac
     9241 gatctgccgg aaagcgcagt aggcgtctcc agcgatgtgg tgatgcttaa cgataacctg
     9301 caaccggaag agccgaaaac ggcgtcgatt ccgattaaca gtaacctgat tgtccacgtt
     9361 ggcaacaatg tgcgcattga cgcctttggc ctgaaagcgc ggctgacggg cgatcttaac
     9421 gtcgttcagg acaaacaagg gctgggcctg aacgggcaga tcaacatccc tgaagggcgc
     9481 ttccatgcct atggtcagga tctgattgtg cgtaagggtg agttactgtt ctctggtccg
     9541 ccggatcaac cgtatcttaa tattgaagct attcggaacc cggatgctac agaagacgac
     9601 gtaatcgccg gagttcgcgt cactggtctg gcggacgaac cgaaagcgga gatcttctct
     9661 gacccggcga tgtcgcaaca agctgcattg tcttatttgc tacgtggaca agggctggag
     9721 agcgatcaga gcgacagtgc ggcaatgacc tcgatgctga ttggtctggg ggttgcgcaa
     9781 agtggccaga ttgtgggtaa aatcggcgag acgtttggcg taagcaattt agcgctcgac
     9841 acccagggag taggcgactc ctcccaggtg gtggtcagcg gctatgtatt gccaggtctg
     9901 caagtgaaat acggcgtggg tatatttgac tctatagcaa cactcacgtt acgttatcgc
     9961 ctgatgccta agctatatct ggaagccgtg tctggtgtag accaggcact ggatttgctc
    10021 tatcagttcg agttttagca atgcgaatat ttgtctacgg cagtttacgc cacaaacaag
    10081 gcaacagtca ctggatgacc aatgcccagt tactgggcga tttcagtatc gataactacc
    10141 agttgtatag cctgggccac tatccaggcg cagttccggg gaacggaaca gtacacggtg
    10201 aagtttatcg tattgacaac gccacgctgg ccgaacttga tgccttgcgc accaggggcg
    10261 gtgaatacgc gcgccagttg attcagacgc cgtacgggag tgcatggatg tacgtttatc
    10321 aacgacccgt cgatggatta aagctaattg aaagcggcga ctggttagac agggataagt
    10381 aaccatatgc atacgccacc ttcgggtggc gttgtttttt gcgagacgac tcgcattctg
    10441 ttttgtaatt ccctcacctt ttgcttttct ctccgagccg ctttccatat ctattaacgc
    10501 ataaaaaact ctgctggcat tcacaaatgc gcaggggtaa aacgtttcct gtagcaccgt
    10561 gagttatact ttgtataact taaggaggtg cagatgcgta ttaccataaa aagatggggg
    10621 aacagtgcag gtatggtcat tcccaatatc gtaatgaaag aacttaactt acagccgggg
    10681 cagagcgtgg aagcgcaggt gagcaacaac caactgattc tgacacccat ctccaggcgc
    10741 tactcgcttg atgaactgct ggcacagtgt gacatgaacg ccgcggaact tagcgagcag
    10801 gatgtctggg gtaaatccac ccctgcgggt gacgaaatat ggtaaagaaa agtgaatttg
    10861 aacggggaga cattgtgctg gttggctttg atccagcaag cggccatgaa cagcaaggtg
    10921 ctggtcgacc tgcgcttgtg ctctccgttc aagcctttaa tcaactggga atgacgctgg
    10981 tggcccccat tacgcagggt ggaaattttg cccgttatgc cggatttagc gttcctttac
    11041 attgcgaaga aggcgatgtg cacggcgtgg tgctggttaa tcaggtgcgg atgatggatc
    11101 tacgcgcccg gctggcaaag cgtattggtc tggctgcgga tgaggtggtg gaagaggcgt
    11161 tattacgctt gcaggcggtg gtggaataag gtgtgtttat ttatcgcggg cataaaaaaa
    11221 cccttactaa ccgaagcccg gcgttcaggg ttattgcgct ataagaactt atttattctt
    11281 ggcgcgctcg aaggaggcaa cgatttcagc tttagcggct tctgcgtttt cccaaccttc
    11341 aactttcacc cacttgcctt tttcgaggtc tttgtagtgc tcgaagaagt gagcgatctg
    11401 cgctttcagc agctctggca gatcgttaac gtctttaatg tgatcgtatt ctttgctcag
    11461 cttggtgtgc ggaacagcaa tcagtttcgc atcttcaccg gcttcgtcgg tcattttcag
    11521 aacgccaacc ggacggcaac ggatcacaga acccggctgc agcgggtacg gagtcgggac
    11581 cagtacgtca accgggtcac cgtccagaga cagggtgtgg ttgatgtaac cgtagttgca
    11641 cggatagaac atcgcggtgg acatgaagcg gtcaacgaac agtgcgccgc tctctttgtc
    11701 gatttcgtat ttgatcggat ctgcgttagc cgggatctca ataacaacgt agatgtcttc
    11761 cggcagatct ttacccgcag ggacgttgag taagctcatg tctgtttcct ttaaaaatat
    11821 gtggcaaaca agtgccgagt attatagcca actcgcgccg aatgtcttcg cttgttttcg
    11881 tcttcatttt cctttttcgc cagttttaag acggtataca gagcaggaaa atccataacc
    11941 gtagccgcat ttttcatagt gagatgaaag cgattacaaa cttgtgatta acgttttatt
    12001 tacttttttg aagtgtgatg taacgcaatc cgttacataa cgaattgtct atagtttttt
    12061 cgcgaacatc ttttcaccaa taataattac cccgacgagg acaaccctat gtggaaacgc
    12121 ttacttgtag tctctgcagt ctcggcagcc atgtcgtcta tggcgttggc cgctccatta
    12181 accgtaggat tttcgcaggt cggatcggaa tccggctggc gcgccgcaga aaccaatgtg
    12241 gcgaaaagtg aggccgaaaa acgcggaatt acgctgaaaa ttgccgatgg tcagcaaaag
    12301 caggaaaacc agattaaagc ggtacgttcc ttcgttgcac aaggggtgga tgcgatcttt
    12361 atcgctccgg tggtcgcgac aggttgggaa ccggtattaa aagaggcgaa agatgccgaa
    12421 atcccggtct tcttgctcga ccgttccatc gatgtgaaag acaaatctct ctatatgacc
    12481 accgttactg ccgacaacat cctcgaaggt aagttgatcg gtgactggct ggtaaaagaa
    12541 gtgaatggca aaccatgcaa cgtggtggag ctgcagggca ccgttggggc cagcgtcgcc
    12601 attgaccgta agaaaggctt tgccgaagcc attaagaatg cgccaaatat caaaatcatc
    12661 cgctcgcagt caggtgactt cacccgcagt aaaggcaaag aagtcatgga gagctttatc
    12721 aaagcggaaa acaacggcaa aaacatctgc atggtttacg cccataacga cgacatggtg
    12781 attggtgcaa ttcaggcaat taaagaagcg ggcctgaaac cgggcaaaga tatcctcacg
    12841 ggttccattg acggtgtacc ggacatctac aaagcgatga tggatggcga agcgaacgcc
    12901 agtgttgaac tgacgccgaa catggcggga cccgcttttg acgcgctgga gaaatacaaa
    12961 aaagacggca ccatgcctga aaagctgacg ttaaccaaat ccacccttta cctgcctgat
    13021 accgcaaaag aagagttaga gaagaagaaa aatatggggt attgagggtt gctatgcctg
    13081 atgccgattc gtaggccgga taaggcgctc gcgccgcatc cggcgatggt gcactgaagc
    13141 ctgatgtgac gcttaccgcg tcttatcatg cctactggga gcacgcttta caccggggga
    13201 aaccatgacg accgaccaac accaggaaat cctccgcacc gaaggattaa gtaaattttt
    13261 ccccggcgtc aaagcgttag acaacgttga tttcagcctg cgccgtggcg aaatcatggc
    13321 gctgctcggt gaaaacgggg cgggaaaatc gacgctaatc aaagcattaa ccggtgttta
    13381 ccacgccgat cgcggcacca tctggctgga aggccaggct atctcaccga aaaataccgc
    13441 ccatgcacaa caactcggca tcggaaccgt ttatcaggaa gtcaacctgc tacccaatat
    13501 gtcggtcgct gataatctat tcataggtcg cgaacccaaa cgtttcgggc ttctacgccg
    13561 taaagagatg gaaaagcgcg ccaccgaact gatggcatct tacggtttct ccctcgacgt
    13621 gcgtgaaccg cttaaccgct tttcagtcgc gatgcagcaa atcgtcgcca tttgccgggc
    13681 tatcgatctc tctgccaaag tgctgatcct cgatgaaccc accgccagtc tcgacaccca
    13741 ggaagtagag ttactgtttg gcctgatgcg tcagttgcgc gatcgcggcg tcagcctgat
    13801 ctttgtcact cactttctcg atcaggtcta tcaggtcagc gatcggatca ccgtcttacg
    13861 caacggcagt ttcgtaggct gtcgggaaac gcgcgagcta ccgcagatcg aactggtaaa
    13921 aatgatgctg gggcgcgagc tggataccca cgcgctacag cgtgccgggc gaacattgtt
    13981 gagcgacaaa cccgttgcag cgttcaaaaa ttacggcaaa aaaggaacga tcgcaccgtt
    14041 tgatctcgaa gtacgccccg gcgagatcgt cggtctggct ggcttgctgg gatcaggacg
    14101 taccgaaacc gccgaagtga tcttcggtat caaacctgct gacagcggca ctgcgttgat
    14161 caaaggcaaa ccgcaaaccc tgcgatcgcc acatcaggcg tcggtactgg gtatcggctt
    14221 ctgcccggaa gacaggaaaa ccgatggcat catcgctgct gcctcggtgc gggaaaatat
    14281 catcctcgct ctccaggccc agcgcggctg gctacgacct atttcccgta aagaacagca
    14341 agagattgcc gaacgcttta tccgccagct tggcattcgc acaccttcaa ctgaacaacc
    14401 gattgaattt ctctccggcg gcaatcagca aaaagtattg ctttcacgtt ggctactgac
    14461 ccgaccgcaa tttctgatcc ttgatgagcc aacccgcggc attgatgttg gtgcccacgc
    14521 cgagatcatc cgcctgattg aaacgctatg cgccgatggt ctggcgctgc tggtgatctc
    14581 ctccgaactg gaagagctgg tgggctatgc cgatcgggtg atcatcatgc gcgatcgcaa
    14641 acaggtggcg gagatcccgc tggcagagct ttccgttccg gcgatcatga acgccattgc
    14701 ggcgtaagga gaacagtgtg atgcctcaat ctcttccgga caccactacg ccaaaaaggc
    14761 gctttcgctg gccaacggga atgccgcagc tggcggcact gttgctggtg ctgctggtcg
    14821 atagcctggt ggccccgcat ttctggcagg tggtgcttca ggatgggcgt ttgttcggta
    14881 gccctatcga cattcttaac cgtgcggccc ccgttgcgct gttggcgatt ggcatgacgc
    14941 tggtgatcgc aacaggaggg attgatctct ctgtgggggc ggtgatggct atcgccggag
    15001 ccacaacggc tgcgatgacg gtcgcgggat tcagcctgcc gattgttttg ttaagcgccc
    15061 tgagcactgg catcctggcg ggattgtgga acggcatact ggtagcgatc ctcaaaattc
    15121 agccgtttgt cgccactctg atcctgatgg tcgccgggcg cggcgtggcg caactgatca
    15181 cctccggaca gatcgtcacg tttaactcgc cggatctctc atggtttggc agtggatcgc
    15241 tattgttcct gccaacgccg gtcattatcg cggtgctgac gcttctcctg ttctggctgt
    15301 tgacccgcaa aacggcgctg gggatgttta tcgaagccgt tggtatcaac attcgggcgg
    15361 caaaaaatgc cggagtcaac acgcgaatca tcgtcatgct cacctacgtg ttgagcgggc
    15421 tatgtgcggc gattgcgggc attatcgtgg cggcggatat tcgcggtgcc gatgccaata
    15481 acgccgggtt atggctggag ctggacgcca ttctcgcggt ggtgattggc ggcggatcgc
    15541 tgatgggcgg gcgctttaac ctgctgcttt cggtggtggg ggcgctgatt attcagggga
    15601 tgaacaccgg aattttgctt tcgggctttc cgccagagat gaaccaggtg gtgaaagcgg
    15661 tggtggtgct ttgcgtgctg attgttcagt cgcaacgctt tatcagtctg attaaaggag
    15721 tgcgtagccg tgataaaacg taacttgccg cttatgatca ccatcggcgt ctttgtgttg
    15781 ggttatcttt actgcctgac ccagtttccc ggttttgctt ccacaagagt gatctgcaat
    15841 atcctgaccg ataacgcctt tcttgggatc attgccgttg gcatgacctt tgtgatcctc
    15901 tccggtggga tcgatctctc cgtcggttcg gtgatcgcct ttaccggcgt gtttctggca
    15961 aaagtgattg gcgatttcgg cctctcgccg ttgctggcat ttccgctggt gctggtaatg
    16021 ggctgtgcct ttggcgcatt tatggggctt ctgatcgacg ccctgaagat cccggcattt
    16081 atcattacgc tggcgggaat gttctttttg cgcggcgtca gctatctcgt ttcggaagag
    16141 tcgatcccga taaaccatcc catttatgac acgctctcaa gccttgcgtg gaaaatccct
    16201 ggcggcggtc gcttaagtgc gatggggctg ctgatgctgg cggtggtggt tatcggcata
    16261 ttcctcgcgc atcgtacccg ttttggtaat caggtatacg ccattggcgg caatgcgacg
    16321 tcggcaaacc tgatggggat ttctacccgt agcactacta ttcgcattta tatgctctcc
    16381 accggactgg caacgctggc ggggattgtc ttctcgattt atacccaagc cggatatgcg
    16441 ctggcgggcg taggtgtgga actggacgct atcgcctcag tggtgattgg cggtacgctt
    16501 ttgagcggtg gcgttggaac ggtattaggg acgctttttg gcgtggcgat tcagggactg
    16561 attcagactt acataaactt tgatggcacg ctgagctcat ggtggacgaa aatcgccatc
    16621 ggtattttgt tgtttatttt tatagcatta cagcgtggat taacggtgct gtgggagaat
    16681 cgtcagagtt cgccagtgac aagagtcaac attgcgcagc gataaaaacg cctctccgtg
    16741 tggagaggcg caggagatta cgcgtccggg aactcacgga taaagcgttc gacatcttca
    16801 accatatggt cgttgccgac aaagaacgaa cggcgctggt gcagggtttc cgggataata
    16861 tccagaatac gctctttgcc atcgctcgct ttaccgcccg cttgttccgc caggaatgcc
    16921 atcgggttgc actcatacag caaacgcagt ttgccgtccg ggtggctggc ggtgcttggg
    16981 tagagataaa taccgccttt cagcaggtta cggtggaaat ccgcaaccag tgaaccgata
    17041 taacgcgagg tatacgggcg attggtggat ttatcttctt cctggcagaa tttaatgtac
    17101 ttcttcaccc cgttcggaaa cttaatgtag tttccttcgt tgatggagta ggttttgcct
    17161 ttctccggga agcgcatccg ttcctggcac aggcagaaaa cgcccagcga aggatcgtaa
    17221 gtaaaggcgt gaacaccgca tccggtagtg taaaccagca tggtagagga gccgtatacc
    17281 acgtaacctg ccgcaacctg tttgttacca ggctggagga aatcttcttc cgttaccggc
    17341 gtgccaacag gcgtaacgcg gcggtagatg gagaaaatgg taccgacaga gacgttaaca
    17401 tcgatgttgg acgagccatc cagtgggtcc atcagcacca cgtattttgc atgttcacag
    17461 ccttcaaaga caacaatctc atcttcttct tcagaggcaa tgcccgcaac gatatcgcgt
    17521 gctttcagtg cggctttcag tttttcatta gcgaacaagt cgagtttctg ttgaacctcg
    17581 ccctgcacgt tctcagcacc gctggcaccc aggatatcaa ccagtcctgc tttgttgata
    17641 tcgcgatgga taatcttggc gcccagtttt attgccgaca gcaaagcagt gagctcaccg
    17701 gtagcatgag aaaactcgtg ctgcttttcg acaataaatt cacctaacgt tttcataaaa
    17761 ctttccctgc aatgtttatg gagtaaagcg accgcaacaa tcttaacaaa taatctcaat
    17821 gttgcgctca ggtgaatcgc gccagcaaat tgcggattat cctgaaatgc gtttctcact
    17881 tgcccgacat atgcgtaaaa tgagcggcag attaaaaaag gatagtgacg tatgcgcatt
    17941 catattttag gaatttgtgg cacgtttatg ggcggtctgg cgatgctggc gcgccagtta
    18001 ggccatgaag taacgggttc ggacgccaat gtgtatccgc cgatgagcac cttacttgag
    18061 aagcaaggca ttgaactgat tcagggttac gatgccagcc agctcgatcc gcagccggat
    18121 ctggtgatta ttggcaacgc catgacccgt ggaaatccgt gtgtggaagc ggtactggaa
    18181 aaaaacatcc cttatatgtc aggtccacag tggctgcacg attttgtgct gcgcgatcgc
    18241 tgggtgctgg ccgttgccgg tacacacggc aaaactacca ccgcgggaat ggcgacctgg
    18301 attctggaac agtgcggtta caaaccggga tttgtgatcg gcggtgtgcc ggggaacttt
    18361 gaggtttcgg ctcgtctggg cgaaagcgac ttctttgtta tcgaagcgga tgagtatgac
    18421 tgcgccttct tcgacaaacg ctctaaattt gtccattact gcccgcgtac gctgatcctc
    18481 aacaaccttg agttcgatca cgccgatatc tttgacgacc tgaaagcgat ccagaaacag
    18541 ttccaccatc tggtgcgtat cgttccgggg cagggccgta ttatctggcc agaaaacgac
    18601 atcaacctga aacagaccat ggcgatgggc tgctggagcg agcaggagct ggtgggcgag
    18661 cagggtcact ggcaggcgaa aaagctaacc actgacgctt ctgaatggga agtcttgttg
    18721 gatggcgaaa aagtgggcga agtgaaatgg tcgctggtcg gtgaacataa tatgcacaat
    18781 ggcctgatgg cgattgcggc ggcgcgtcat gttggtgtag cgccggcaga tgccgctaac
    18841 gcgctgggtt cgtttattaa cgctcgccgc cgtctggagt tgcgtggtga agcgaatggc
    18901 gtcacggtat atgacgattt tgcccatcat ccgacggcga ttctggcaac gcttgcggcg
    18961 ctgcgtggca aagttggtgg tacggcgcgc attattgctg tgctggagcc gcgctcgaat
    19021 accatgaaaa tggggatctg caaagacgat ctggcacctt cattaggtcg tgccgatgaa
    19081 gtcttcctgc tgcaaccggc gcatattccg tggcaggtgg cagaagtggc agaagcctgc
    19141 gttcagcctg cacactggag tggcgatgtg gatacgctgg cagatatggt ggtgaaaacc
    19201 gctcagcctg gcgaccatat tctggtgatg agcaacggcg gttttggtgg gatccatcag
    19261 aaactgctgg atggtctggc gaagaaggcg gaagccgcgc agtaattcgg ccttagcctg
    19321 atatagtgtg cgtaggccgg ataaggcgtt cacgccgcat ccggcatttg agcatagtgc
    19381 ctgatgcgac gcttgatgcg tcttatcagg cctacaggtg caccgcatcc ggcaaaccat
    19441 actcacatca acaacgaaaa ttacccttca ttctccgcca gttcgcgcag atactggaaa
    19501 atctggcgtg cggatttcgg cggcttattc ccttctttct ctttcttcgc gttgcggatc
    19561 agagtacgca gttgctgacg atcggcatcg ggccacagat ttaatacctc agcgatggcg
    19621 tcatcacctt gatcgatcag acgatcgcgc aggttttcca gtttatggaa cagcaccacc
    19681 tgctggttgt gacggttttt cagcttatcc agcgcctgac ggatcggttc tacatcgcgc
    19741 tggcgcagca tcttaccgat gagctgcaac tggcggcggc gaccttccat cttaatacgc
    19801 tgggcgagct caatagccgc gcgcagatcc gcatctaacg ggatcttatc cagcgcgttt
    19861 ttccccagat caacaatttc cgcgccaagg cgttttagct cctcggcatc acgtttaatt
    19921 tcacttttac tgacccagat aatttcatcg tcttcgtctt cgatgtcatc accgggaacg
    19981 tcgtcgagcc agtcttcggg ctgcttagtc atctcaggct ccttaaaaaa agaggctaat
    20041 gttaccagtt aagatgcgca ctgaaaaacg gttctctgtt agacttcaga gaaactctct
    20101 acattatggc acttgcaatg aaagtaatct ctcaagttga agcgcagcgc aagattctgg
    20161 aagaagcagt ttccactgcg ctggagttgg cctcaggcaa atcggacggt gcggaagttg
    20221 ccgtcagcaa gaccaccggc attagcgtaa gcacgcgtta tggtgaagtg gagaatgttg
    20281 aattcaatag cgatggcgcg ctggggatca ctgtttatca ccagaaccgc aaaggtagcg
    20341 catcatccac cgatttaagc ccgcaggcca ttgcccgtac tgtacaggcg gcgctggata
    20401 ttgcccgtta tacctcgcca gatccctgtg ccggcgtggc agacaaagag ctgctggcct
    20461 ttgacgcacc agatctcgac ttgttccacc ctgcggaagt ttccccggat gaagccattg
    20521 aactggcggc ccgcgcagaa caggcggcat tgcaggcgga caaacgcatc accaataccg
    20581 aaggtggcag ctttaacagc cactacggtg tcaaagtttt tggcaacagc cacggcatgt
    20641 tgcagggtta ctgctcaacg cgtcattcgc tctccagctg tgtaattgcc gaagaaaatg
    20701 gcgatatgga gcgtgattac gcctacacca ttggtcgtgc gatgagcgat ctgcaaacgc
    20761 cagagtgggt tggggccgac tgtgctcgcc gtactttatc gcgcctgtca ccgcgtaaac
    20821 tctccaccat gaaagcgccg gtcatttttg ccaatgaagt ggcaaccggg ctttttggcc
    20881 atctggtggg ggcgatagcg ggtggatcgg tttaccgtaa atctaccttc ctgctggatt
    20941 cgctgggtaa acaaattctg ccggactggc tgaccattga agagcatccg catctgctga
    21001 aagggctggc gtcgacgcca ttcgacagcg aaggtgtgcg caccgagcgt cgcgatatta
    21061 ttaaagatgg catcctgact cagtggctgc tgaccagcta ttcggcgcga aaactggggc
    21121 tgaaaagcac cggacatgcg ggcggtattc acaactggcg gattgccgga caaggtctaa
    21181 gcttcgagca gatgctcaaa gagatgggca ccgggctggt ggtgacggaa ttgatgggcc
    21241 agggcgtgag tgcaattacc ggtgattatt cccgtggtgc ggcgggcttc tgggtagaaa
    21301 acggcgaaat tcagtatccg gttagcgaaa tcaccatcgc aggtaattta aaagatatgt
    21361 ggcgcaatat tgtcaccgtc ggtaacgata ttgaaacacg cagtaatata cagtgtggtt
    21421 ctgtgctgtt gccggagatg aaaatcgccg gacagtaaaa aaactggcgc gacctgccgc
    21481 gctataaact aaaaaattac acaaaatcat tcgcactgca tcgagacgga aagtgaatga
    21541 gggccgggga gcgtactcgc agtacgtgac ccggtcgaat gagcgtagtc gtcgatgagg
    21601 cagtgtgaag gatgaagtgt aaataaaaaa ggaagtgagc aatgcgaaaa agcctgttag
    21661 ctattcttgc agtctcctcg ttggtattca gttcggcgtc gtttgccgct gatcttgaag
    21721 acaatatgga aaccctcaac gacaatttaa aagtggtcga aaaagccgat aacgcggcgc
    21781 aagtcaaaga cgcgttaacg aagatgcgcg ccgtagcgct ggatgcgcaa aaagcaacgc
    21841 cgccgaagct cgaaggcaaa tcaccggaca gcccggagat gaaagacttc cgtcacggtt
    21901 tcgatattct ggtcggtcag attgacgacg cgctgaagct ggcaaatgaa ggtaaagtaa
    21961 aagaagcgca ggctgccgca gagcaactga aaacgacccg caacgcctat caccagaagt
    22021 atcgttaatt cctcatttcc ctgttgccta cactcaggta acagggaact tatcatcgca
    22081 aatgatgcac cacctgattg ctgctgccgc gccagatcag ggaagggtct tttaaatcct
    22141 gcacaaattt gccgtcgacc agcacgttaa tcaaatcaac aacctgcatt tgcgcagcgt
    22201 tgagttcgtc gagtttatag cctgtccaca cccagatgtc tttacccgga cactcggcgc
    22261 ggatgcgttg taccagtttc agaatatccg gcacgttttg cggatgcagc ggatcgccgc
    22321 cggagaggga aataccctgg cgtttgatac gagtgtcatt cagatcgtta atgatctggt
    22381 cttccattgc tttagtaaat ggctgaccgg aatttacccg ccatgtgctt ttgttatagc
    22441 aaccggggca ttcatgaaca cacccggaga caaacagggt gcagcgagtg ccgggaccgt
    22501 tgacgatgtc gacaggatag tactgatgat aattcattat ttcgcctggc ttatcagtcc
    22561 gcgtgtaggc tgtatcaggc aatcaacgcc tgatgcgacg ctgtcgcgtc ttatcaggcc
    22621 tacagccgtt gccaaatgta ggccggataa ggcgtttacg ccgcatccgg caatcaacgc
    22681 gggatgcgac gctttcacat cacatccggc atttgcttag tgcagatcaa cctatctgcc
    22741 cattccccaa atgtttaacg cggcgcttaa cttcttcctg cttaccggcg ttaaacggac
    22801 gtgcatccgg gctacctaaa tatccgcaca cgcggcgagt taccgacaca cgggaggcgt
    22861 catggttacc acatttcggg caagtgaagc ctttgctggt gcactcgaac tcaccggtaa
    22921 agccacactc gtagcactca tcaatcggtg tattggtgcc gtaatacggt acatgctgat
    22981 agctgtaatc ccagacatct tccagcgcct tcaggttgtg ctgaatgttt ggatactcgc
    23041 cgtagcaaat gaaaccaccg ttcgccagcg gcgggtaagg cgcttcaaag tcgatcttgt
    23101 cgtacgggtt caccttcttc tccacatcga ggtggaaact gttggtgtag taacctttat
    23161 cggtcacgcc cggcaccacg ccaaactcag cagtatcgag acggcagaag cgatcgcaca
    23221 ggttttcact cggcgtgctg tagagactga aaccataacc cgtttcttct ttccactgat
    23281 ccactgcctg acgcagacgt tcaacaatgg cgataccttt cgcgcgaagc tgctcgttgt
    23341 cgtaaacatg ctcgccgccg aacagcgcgt taatggtttc gtggatgccg atgtaaccca
    23401 gcgaaataga cgcacgaccg tttttgaaga tttcagaaac atcatcgtca gcattgagac
    23461 gcacgccaca agcaccttcc atatagagga tcggggccac gcgcgctttc acgccttcga
    23521 gacgagcgat acgggtcatc agcgccttac gtgccagcac cagacgttca tccagcagct
    23581 tccagaaggt ggcttcatcg ccttttgctt ccagagcaat acgcggcagg ttcaggctga
    23641 tcacgccgag gttgttacga ccatcgtgga tctgctcgcc gttttcattt tcccacacgc
    23701 cgaggaagct gcggcagccc atcggagttt taaacgaacc ggtgactttc actacctgat
    23761 cgtagttcag gatatccgga tacatgcgct tgcttgcgca ctccagcgcc agctgtttga
    23821 tgtcgtagtt cggatcgcct tttttatggt tcaggccatc gcgaatcgca aacaccagtt
    23881 tcgggaacac cgcagtttta cggtttttac cgaggcctgc gatacggtta cgcaggatcg
    23941 attcctgaat caggcgcgat tcccagctgg tgcccaggcc aaaaccaaag gttacaaacg
    24001 gcgtctgacc gttggcggtg tgcagggtgt ttacttcgta ctccagtgac tggaaggcat
    24061 cgtagcactc tttgatggtt cgagagttag cgtagccttc ggcgtccggg atgttccact
    24121 cttctgcggt cttgcgatgt ttgttgtagc tggcagtgac aaacggtgcc agcacttcat
    24181 cgatacggtt aatggtggtg ccgccataaa tatggctggc aacctgagca ataatctgcg
    24241 cagttaccgc ggttgccgta gagatcgact tcggcggttc aatctcggcg ttccccattt
    24301 taaagccctg ggtcagcatg cctttcaggt cgatcaacat gcagttgaac atcgggaaga
    24361 acggtgagta atcgagatcg tgatagtgaa tatcgccacg ctcatgtgcc tgcaccacgt
    24421 cacgcggcag caggtgctga cgtgcatagt gtttagccac gatcccggcc agcaggtcgc
    24481 gctgggttgg aatcaccttg ctgtctttgt tggcgttttc gttgagtaac gaggcgttgg
    24541 tctgctcgac cagaccacgg atctcctgat tcaggcgacc gcgtttttca cgttcaatgt
    24601 cgcgatcgtg acggtactcg atgtaagcac gagccagttg tttgtatgga cctgacatca
    24661 gctggttttc aacagcggtc tggatctcat tgatatccac ctggttgcgg ccctgcatct
    24721 gctcgctgac aactgcagca acagtggcgc aataatcggc atcatcgact tccgctgctt
    24781 tagctgcacg cagaatcgct tctttgatgc gctctgattt aaacggcact ttgcagccgt
    24841 ctcgtttcat cacatgcggt gtcatgatcg ctccatattt ttaagaacag gttatccaca
    24901 gaaattggga aaggcgtttt ccggtttttt cgtttcgttt tccggtgctt tccgcaatcg
    24961 ccatccgctt atccacaacg ccgcaccttt ttcgtgggga gctgtagtag caattatagt
    25021 cgattaatac aacatattgg gttgggacgc attttaaagt ctatatatag tgctttgcat
    25081 caaggatgtt tgaacttttt ttgatgtagc tcaaagtaaa aagcagagcg tacggatgac
    25141 gggcgctaca gcgttatgta aattttttaa tgaatttgct ggttgaaaaa tcaacaaaaa
    25201 caacatactg acagacaaaa ccccggaatg accggcatta ccggggctta gggaagattt
    25261 acttctgtaa ccaccagaca gcctcaaaag gccgtaaagt catggcacag ggttgtggtg
    25321 aggcttcttc gtagttatgc atcacaagct gccagttgcc gcgcatttgc tctggctgcc
    25381 aggggtggat ctcacggcta aggttggcaa tgaccagcaa ggtttgcccc ttccattcac
    25441 ggcgatagca ccacaataca gggctgtttg gcagcagatc ctggtaattg ccccatgtca
    25501 ggatggcttc ctgcttacgc agtgcgatta acttttggta ggtgtaaaac accgaggaat
    25561 catcggccaa cgcggcttct acgttgattt gttgatagtt atcgcccagg ccaatccacg
    25621 gttcgccagc cgtaaacccg gcattatcgc cgttgctcca ttgcatgggc gtgcgactgt
    25681 tgtcacggga tttactggcg aggattgcca ataactcgtc ggcatcacgc ccatcgttgc
    25741 gcagctcggc aaacatattg aggctctcta cgtcgcgata gtcagtaatg cgcgtgaaat
    25801 gcgggttggt catgccaatc tcttcgccct ggtagatata cggcgttccc tgcatgccat
    25861 gcagcaccat cgccagcatt tttgccgcag gcacgcggta ttcaccttca tcaccaaagc
    25921 gagaaacaat gcgcggctga tcgtggttac accagaacaa ggcattccat gccacgttgt
    25981 gcattccttg ttgccagtgg cggaacaatt ttttcaacgc cacaaagtca ggtttagcca
    26041 gcgtccattt ttcaccaccg ggataatcga ccttcaggtg atgaaaatta aaggtcatcg
    26101 acaattcact gcctgtcaga gccgcgtatc gctggcaatg ctcaaggctg gtggaggaca
    26161 tttcacctac ggtcattaac ccacgtggcg taaacacatc gcggttcatc tcgtgcaaaa
    26221 actcgtgtgc tcgtggcccg tcggtgtaga agcgacgccc gtcgccatcc aggtcatcag
    26281 ggaaacgcgg gtctttggag ataagattca ccacatccag gcgcaacccg tcgaccccac
    26341 gatcggccca gaactcacag acttttttca gctctgcgcg taccgctgga ttctcccagt
    26401 tgagatccgc ctgttctggt gcaaagagat gcaaatagta ctgttcgctt tccgcatgcc
    26461 agcgccacgc actaccgcca aattttgaac gccagttgtt cggtggcgtt tctggttcgc
    26521 catcgcgcca gatataaaac tggcggtaag ggctttcttt gttcagcgcc tcgcgaaacc
    26581 aggcatgttg ggtagaggta tggttaaaca ccatatcgag aatgatacga atcccgcgcg
    26641 attttgcctg cgtcaccagt tcgtcaaaat cgtccagcgt gccgtaggtg ggatcaatcg
    26701 ccgtatagtt cgctacgtcg taaccgttat cgacctgggg agagacataa aagggggtta
    26761 gccagatggc atcaacgccc agtttatgca gatagtccag gcgttggata acgccacgta
    26821 aatcgccggt accgctaccc gtggtgtcct gaaaactctt tggataaatc tggtagataa
    26881 cgccgttttg ccaccagtgg ggaagattag tcattacgtt attcctgcga atgcgagggg
    26941 gcgcaattgc gccccgaaga aaattaaaca atgtccagcg tgcccaggcg gtatttccgc
    27001 tgatagataa acgaggtgag tacaatcggg atgatgatgg cgatagccat tgccagcgca
    27061 aacacctgcc agtagctcgg ttgaatcgag agaattcccg gcaggccgcc tacgccgatg
    27121 ccattcgcca taacgccgtt caggccgcat agcaatcctg ccagaccaga accaatcatc
    27181 gcacacagca tcgggaagcg atatttcagg ttgatgccgt acattgcagg ctcagtgacc
    27241 ccaagccagg cggagatcgc cgcaggcacg gagatctcgc gttcattgtg cttgcggctg
    27301 gaaatgataa tgcctatcac ggcggagccc tgagcgatat tcgacagcgc aatcagcggc
    27361 cacactggcg tgccacccat gctttgaatc atctgcaaat caatagcaag cgtggtctgg
    27421 tgtaccccgg tgatcaccag cggggcgtac aggaagccaa acaatgccgc gccaatcgga
    27481 gcaaagctgc cggtcatcag gtgacgtacc gcaaaggcaa cgccatcgcc aatcatgcga
    27541 ccaaacggac caatcagcgc atgggcgagg aacaccgcga ggatcagcga acagacgggc
    27601 accaccacca gatagaggta atctggcacg atgcgtttaa ggcgagtttc aataacgccc
    27661 agcgccagcc cggctaatag tgccgggatc acctgcgcct gatagcccac tttggcgatg
    27721 ctgaacatgc caaagtccca cacttccggc agctgctgcc cgagcagata agcgttcatc
    27781 agctgtggag aaaccagcgt tacgccaagt acgataccaa ggatcggcgt gccgcccatt
    27841 tttttcaccg ctgaccagca aatcccgacc ggcaggtaga agaagatcgc ttcaccgatc
    27901 aaccacagaa aatcgtagat cgtttgcagg gaagggtaca tttgtgccag cgtttgaccg
    27961 ttgctcatgg gcaaatcgcc gatcacattg cgaaaaccga ggatcaaacc gccgctaatc
    28021 aacgcgggca gcaacgggaa gaagatctct gcgaaatgag agatcaactg ctcatgccat
    28081 ttcatattct gccgggcggc tttttttacc tgctctttat caacctgcgc ctgtccggtt
    28141 gacgctatca gtgcttgata gtaatcaccc acgttggtgc caatcaccac ctgaaattgc
    28201 ccggcattgg tgaaacagcc tttcaccatg gggagttgct caatttcttt cggtctggca
    28261 ttggccggtt ggttgaggac aaagcgcagg cgagtaatac agtggctcac cgtcgcaata
    28321 ttgccgcgcc cgccgaccag ttcaatcaac cgatcgatat ccgtttggtt tattttgctc
    28381 atcataaagc cccatggcag atgacatttt tggttggctg cagaatatat tgcgcggaaa
    28441 aatttaaaaa cgggaacgtt cccgaaacgc agcgaagatc acaatttatc gttcaggaaa
    28501 cgatcaggac agggtggcgg ggatgatgat ttgttgcggt tcgctgcgcc cggttacctg
    28561 tgcgatcaac tggcaggccg cctggcgtcc agcttcggcg taaccaggat ctacggtgac
    28621 gatctccgga tggaggaatt tcattaacgg cgtattaccg acgctcgcca gttgcaaggt
    28681 gtcgatgcgt tgctcttgca ggtatttact tgcgccaagt gccagcgtgt cggttgcgca
    28741 cagtaaggcg gtagtttcag gcgtaatcac ttttgctacg ttctcatagc cttgcttcat
    28801 agcaaggcct ggcagggcgg caacgggatg cagtttatgc gctttgcaga acgccaggta
    28861 ggcttcgtga cgtcgcttac cggttgtcac gtcactgtgc ggcacgccga gataactgat
    28921 attacgatgc ccctggtcat acagccgttg catcaggatc ttgattgccc cttcgtcgtc
    28981 ataacagacc gaagcaaagc cttttgcgtc acgcgccagc agaaccagcg atgactgcca
    29041 gtgggctaac atttcttctg ttatgccagt aaaaccgaac agcactacgc cgtcgatatt
    29101 acgccgtttc agcaccccca aatgttcggc aactaattgc ggggaaaact gactttccat
    29161 catgattggg tcgtaacctt gttcatagaa cgctggcagc atggtttgaa cggcgagatt
    29221 ttctgacaac gaatccagac gggtaacaat gatggcgacc actttatcgc tttgcccacg
    29281 catagcgcgc gcagagcggg aaggggaaaa tccatgctga ttcatcactg cttcaacacg
    29341 ctcgcgggtg cgctggctca cgccgctttc gttattcagc acccgggaaa ctgtagattt
    29401 ccccacgccg cttaagcgcg cgatgtcttt gatggtcagc cgattttgca tcctgttgtc
    29461 ctgtaacgtg ttgtttaatt atttgagcct aactttaccc gtgcattcag caatgggtaa
    29521 agtctggttt atcgttggtt tagttgccag caggtattat atcgccatag atgctacgaa
    29581 tattattgga ttctccttat tatttgcggc gcttttttca cttaccggag gttatatgga
    29641 acctgatccc acgcctctcc ctcgacggag attaaaactt ttccggtaag cccgtctttt
    29701 cacggcgtta ccggatgcgt aaggccgtga cgttttaacg tccctgctca gctttattac
    29761 cttcaggtaa ggcttcgcca cgcctgaaga catttctgta ctgtttcaga cagtgcggag
    29821 ggactcctta tgtttaaaga aatttttacc cggctcattc gccatttacc ttcccgtctg
    29881 gttcatcgtg atccattgcc tggcgcgcag cagacagtga atacggtggt cccgccgtcc
    29941 ttaagtgcgc attgcctgaa aatggcggtg atgcccgaag aagaattgtg gaaaacgttc
    30001 gacacccatc cggaagggtt aaatcaggcg gaagtggaat ctgcccgcga acaacatggt
    30061 gaaaataaat tacccgcaca acaaccgtcg ccgtggtggg tacatttatg ggtctgctat
    30121 cgcaacccct ttaatatttt actcaccatt ctcggcgcta tttcttacgc cacggaagat
    30181 ttatttgctg cgggcgttat cgcgctaatg gtcgctattt caacgttgct gaactttatt
    30241 caggaagcac gttccactaa agcggcagat gccctgaaag cgatggtcag caataccgcg
    30301 acggtgctgc gcgtaattaa cgacaaaggc gaaaatggct ggctggagat cccgatcgac
    30361 cagctggtgc ccggcgatat tataaaactg gcggcggggg atatgatccc ggcagattta
    30421 cgtatcttgc aagcgcggga tctgttcgtc gctcaggcgt cgttaaccgg tgagtctctg
    30481 cccgtggaaa aagccgctac cactcgccag ccggagcaca gcaatccgct ggagtgcgac
    30541 acgctgtgtt ttatgggcac caccgtggtg agcggcacgg cacaagcaat ggtgattgct
    30601 acaggtgcca atacctggtt tggtcaactg gcggggcgtg ttagtgagca ggaaagcgag
    30661 ccgaatgcct ttcagcaagg gatcagccgc gtcagtatgc tgctgattcg ctttatgctg
    30721 gtgatggcac cggtggtgct gttaatcaat ggctacacca aaggcgactg gtgggaagcg
    30781 gcgctgtttg cgctttcggt cgcggtaggc ctaacgccgg aaatgttgcc gatgattgtt
    30841 acctcgacgc tggcgcgcgg cgcagtaaag ctgtcgaaac agaaagtgat cgtcaaacat
    30901 ctggatgcta ttcagaactt tggcgcaatg gatattctgt gcactgataa aaccggcacc
    30961 ctgacgcagg ataaaattgt gctggagaat cataccgata tctccggtaa aaccagcgaa
    31021 cgcgtgctgc atagcgcgtg gttgaacagt cattatcaga ccggacttaa aaacctgctc
    31081 gatacagcgg tgctcgaagg tacggatgaa gagtcagcgc gctcgctggc cagtcgttgg
    31141 caaaaaatcg atgagattcc gtttgatttc gagcgtcgcc ggatgtcggt ggtagtggca
    31201 gaaaataccg agcaccatca gctggtttgc aaaggcgcat tgcaggaaat cctcaatgtg
    31261 tgttcgcaag tgcgtcacaa tggcgagatt gtaccgctcg atgacaccat gctgcgtaag
    31321 attaagcggg ttactgatac gctgaatcgt caggggctgc gcgtggttgc ggtggcgacg
    31381 aaatacctgc cagcgcgtga aggagattac cagcgggcgg atgaatccga cctgatcctc
    31441 gaaggatata ttgctttcct tgatccgcca aaagagacaa ccgctccggc actgaaggca
    31501 ttaaaagcga gtggaattac cgtaaaaatc cttactggcg acagtgagtt agttgcagcg
    31561 aaagtgtgcc atgaagtggg gctggacgcg ggagaggtgg tcattggtag tgatattgaa
    31621 acgctatctg acgacgaact ggcaaatctt gcacagcgca ccacgctgtt tgcccgcctg
    31681 acgccgatgc ataaagaacg cattgtgacc ttactgaagc gcgaagggca tgtggttggc
    31741 tttatgggcg atggtattaa tgatgcgccc gccttacgcg ctgcggatat cggcatttct
    31801 gtggacggcg cggtagatat tgcccgtgaa gcggctgata tcatcctgct ggaaaaaagc
    31861 ctgatggtgc tggaagaggg ggttattgag ggacgtcgca ctttcgccaa catgctgaaa
    31921 tacatcaaaa tgacggcgag ttctaacttc ggtaatgtgt tcagcgtgct ggtggcgagt
    31981 gctttcttgc ccttcctgcc gatgttgccg ttacacttgc ttattcagaa cctgctgtac
    32041 gatgtgtcac aggtggcgat cccgtttgat aacgtcgacg acgagcaaat tcaaaagccg
    32101 cagcgttgga atccggcgga tctggggcgc tttatgatct tcttcggacc gatcagctcg
    32161 atcttcgata ttttgacgtt ttgcctgatg tggtgggtat tccatgccaa cacgccggaa
    32221 acgcaaacgc tgttccagtc gggatggttt gtggtgggct tactgtcgca aacgctgatt
    32281 gtgcatatga tccgcacgcg ccgtgtgccg tttattcaga gctgtgcatc gtggccgtta
    32341 atgatcatga ccgtgatcgt gatgattgtc ggtatcgcat tgccgttttc accgctggcc
    32401 agttatctgc agctgcaggc gctgccgtta agctatttcc cgtggctggt tgcgattctg
    32461 gcagggtata tgacattaac ccagttggtg aaagggttct atagccgtcg ttacggctgg
    32521 caataaagaa tcaactgggc atggtgaccc ggattcagat tgcagacaaa gtgagttttg
    32581 ttcatgccgg atgcggcgta aatgccttat ccggccaaca aattcaataa attgcaggaa
    32641 tcacgtaggc ctgataagcg tagcgcatca ggcgattttg cgtttgtcat cagacttaat
    32701 ccgggcatga tagcccggat ttccatcaag attagcgacg aacagcgatc gcttcgatct
    32761 caatcttcac gtctttcggc agacgggcaa cttcaacgca agaacgtgcc gggaaggtgg
    32821 cgttgtgttc ggtgaagaag gcttcgtaag tggcgtttac ggttgcgaag tcgttcagat
    32881 cttttacaaa cacggtagtt ttaacgatgt cgcccacttt cagaccagcg gcttcgacga
    32941 tcgcttttac gttatccagc gactgacgtg cctgtgcagc gacgtctgcc ggtacttcgc
    33001 ccgttttcgg atttaccggg atctgaccgg aggtgatgat catattgccc agatcaacgc
    33061 cctgtacgta aggaccgata gctgccggtg cattttccgt cgcgatagtt ttgctcatga
    33121 tttctccttt attacagcgg taaaaagtct ggctcattat agggagccag acttttatta
    33181 ccaaccgcaa ttaattggcc agcaccacat tatgggaaaa ctctttttca cagtatttgc
    33241 atttgagcgc gatgtccttg gcgcgttttc gcacggcaaa gctggatgaa accggttcgg
    33301 catggctgat acagttgctg ttcgggcaga ccagcacatt gtcgatgcgc tccggcagac
    33361 ttgggcgcga tttacccacc acttcatagt tgtcgatacg gttaaccgtg gcttgcggcg
    33421 catacaatgc cagttgatct acctggtctt cactcaaaaa ggtattttcg attttgatca
    33481 gatctttgcg gcccatctcg ccagaaggca ggttcagacc gatggtgatg cgctgatccg
    33541 tttcggtcag cttgaacaga ctcaacagct taaaaccgat ctgggcgggg atatggtcaa
    33601 ttaccgtgcc gcgtttaata gcttcaacct gcaatttatt atcgtgtgtc atctctattt
    33661 ccccttacag taccagatcg cgattcagaa ccagtgccag taacgcctgg cgagcgaaaa
    33721 tcccgttgcc tgcctgctgg aagtaccagg cgtgtggcgt tttatcaaca tccgtcgcaa
    33781 tctcatcaac acgcggcagc ggatgcagca ctttcatatt ggctttggcg ttgtggagat
    33841 cgctggcgcg aagaacaaac tgcgctttca cgttggcgta ctcggacggg tccagacgct
    33901 ctttttgcac gcgggtcatg tacaggatgt caacttccgc catcacttct tcaatagagc
    33961 tgtgcagact ccaggcgatc cctttttcat caagcatatc cagaatatat tgcggcattg
    34021 ccagcgcgtc cggcgcgatg aagtaaaaac ggttgccgtc gaacttcgct aacgcctgag
    34081 tcagggagtg aacggtgcgg ccatatttca ggtcaccaac cattgcgacg tggagattgt
    34141 ccagacgccc ctgggtttcc tgaatagtga ataagtccag caaggtttgc gtcggatgtt
    34201 ggttggagcc atcaccggca ttcagtaccg gtacattgcc ggaaaactcg gtggccaggc
    34261 gcgccgcacc ttcctgcgga tgacgcatca ctatcgcatc aacgtaagtg ctgataaccg
    34321 aaatggtatc ggccagcgtt tcgccctttt tacccagtga tgtattggcg ctgtcggaaa
    34381 aacccaccac gctggcaccc aggcggtgca tggaagtttc gaaagagagg cgggtacggg
    34441 tagaggcttc gaagaaacag ctggcaatga ctttgtgctt caacagctct ggttgcgggt
    34501 ttgctttcag tttcgccgct gtcgccagca ccagattaag atcatcgcga ctaaggtcgt
    34561 ttatggaaat gatatgtttc tgatatagcg gattagccat cttttatctc ctgacgcctg
    34621 ggcaaaaaaa agcccctcga ttgaggggct gggaatgggt gatcaacggg aagaaaaacg
    34681 gcaggccagc gtcttttttc agacgcggta agacaaaatg tcgaacacac tgaaccatac
    34741 atcctcccgg caaattgtcc ggcattatac tcatcgtcag aagcggtgca agcatttgat
    34801 gcaatatttt gtcagcgcaa acggtttata tgaatttaaa gtcaaaatat atgcattttt
    34861 atttactgtg attctggagg ggaactgtcg ttcggtatca ataattgcag acatttcctg
    34921 cttttttctt tttcactttc acgcaatcag atatgcattt tattccattc atccgactta
    34981 tagggcggag tgtgaaaacg aacggctaac actattgctt actgctcagg gatgcgcgct
    35041 atcactttaa tttcaaaatc aaagcctgcc agccatgtaa cacccaccgc cgtccagttt
    35101 ggataaggtg gggcgctaaa tatttcattt ttcaccgtca tgatgtcttc gaattgtttt
    35161 tctggatcgg tatgaaagct cgtaacatca atgatatcgt caaaagtgca tcccgcagct
    35221 gccagggtcg catgcaaatt atcaaatgcc agtctgactt gttgctgaaa atcgggttct
    35281 ggtgttccgt cctctcgact tcctacttgc ccggaaacaa acagcaaatc gccggaacga
    35341 atagccgcag aataacgatg ctcagcatac agtgaatgtc gtccagcagg gaaaacagcg
    35401 gttctttcta ccatttggtt atcctcaaga tttacgacat gaacagaaga tttatattta
    35461 ccaggagccg cttttagcgg acgacgtgag taaacaaaac ccagacatca tgggaaatgg
    35521 ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg ccatcaatgg tatgcatcgc
    35581 gccggtaaca aaactggctt ctggccctgc taaccatgcg accataccag cgacctcttc
    35641 cggttgccca tgtcttttga tggccataaa accatgcaac atatcgcgca ttggcccgtt
    35701 ggcgggatta gcgtcggtat caattggccc tggctggacg acgttgatgg tgatcccacg
    35761 cggtccaaaa tcacgggcca gcccgcgcgc catgccttgt agggcagatt tactggcggc
    35821 ataagcagcc atgcctgcaa caggcatacg atcgccattc acggagccga tgattaagat
    35881 gcgcccgcct tcgggcatct gccgggcggc ttcaacagag gcatggtaag gagcatgaat
    35941 attgattttg aaaaggcgat caatatcgtc ggcatttaat tccagggcat cgccaaagac
    36001 gccaatacct gcatttacca ccaggatatc caatgagccg ctcttacgaa cgacatcaat
    36061 gacagcgtct ctgtcagcac tatctgtgaa tactgctgtc gctccagtct cttgtgccag
    36121 gtgttcagcg gcatctttcg accccgcata ggtgaatcgt acattggccc catcggtgac
    36181 gaaacgacgt acgatagcgg caccgatacc acgactgcca ccgaggatga gaactgtctt
    36241 acctgtaaaa gcgcccataa ggactccttg atttattatg taacatgcat tacaaaactg
    36301 ttttaacttt ctgtcaacaa gttttgtaat ggtcactaaa aaacaatctc gcgttccagg
    36361 tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg gcaaaagtgc tttttcacca
    36421 aaaaggtttc gatgctgtca gtgttgctga agttactgat tatcttggta ttaacccccc
    36481 gagcctctac gcggcttttg gcagtaaggc tgggttattt agccgtgtac tcaatgaata
    36541 tgtcggtacg gaagctattc cgcttgccga tattcttcgt gatgatcgtc cggtagggga
    36601 gtgcctggct gaggtattaa aagaagcggc gcgcagatat agccaaaacg gcggctgcgc
    36661 tggctgtatg gttcttgaag gcattcatag tcatgatcca caagcgcgtg atattgccgt
    36721 tcaatattat cacgccgcag aaacggccat ttatgactat atcgccaggc ggcatccaca
    36781 aagcgcacaa tgtgtgactg attttatgag taccgtgatg tcagggcttt ctgcgaaggc
    36841 acgggagggg cactccatcg aacaactctg tgcaacagct gcactggcgg gggaagcgat
    36901 aaaaactatt ctcaaggagt gatgcgggcc ttgacccgaa aggcgggaac gcgcctgccg
    36961 ataagttgtg ataagacaat aattcacgcg ttaaggctag cggaattgat catcttttcg
    37021 tataacgatg gaaatgaaac gttgttttaa ttaaggagtg gaaaagatga tcatcggaaa
    37081 tattcataat cttcagccgt ggctacccca ggagttacgc caggcgattg agcatatcaa
    37141 agcacacgtt acggcagaaa cgccaaaggg caagcacgat atcgaaggca atcgtctgtt
    37201 ttatcttatc tcggaagata tgaccgagcc gtacgaagct cgccgtgcgg agtaccatgc
    37261 ccgctatctc gacattcaga ttgtgttaaa aggtcaggaa ggcatgacct tcagcacaca
    37321 accggcaggt acgccggata ccgactggtt agctgataaa gacatcgcat ttttgccgga
    37381 aggtgttgat gagaaaacag ttatccttaa tgaaggtgat tttgttgtgt tttatccggg
    37441 ggaagtgcat aaaccgctgt gcgcagtggg cgcgccagcc cgggttcgca aagcagtagt
    37501 gaagatgctg atggcgtgat gacttttcgc cgtaaataac tccaggttta cggcgagttt
    37561 gtgaaaagag cgttttttga tatttttttg tgagtaaaat ttgtaatgct tagacgttct
    37621 tattaactca aggagttcgt catgagcaaa atatcagatt tgaattattc tcaacacatt
    37681 acattagccg acaattttaa acaaaaaaat gaagccttag atacctggta tgtgggaatg
    37741 aatgattttg cccgaattgc cggggggcag aatagcagaa gcaatattct ttctcctaga
    37801 gcatttttgg agtttttggc aaagatattt accctgggtt atgtggattt tagcaaacgc
    37861 tccaacgaag cgggtagaaa tatgatggct catattgagt cctcatctta ttctaaagat
    37921 actgatggca atgaaaaaat gaagttttac atgaataatc ctgaagggga gcgagcggat
    37981 ttatctaagg tgaagataga aattacactt gcctcagctt ctactaaagg gattcgtgaa
    38041 ggacatacag tcattatatt taaacaatct gatggttcga ctaatcgtta tgaagggaag
    38101 tcttttgaaa gaaaagatga tagttcatta cacctgatta ctaacaaggt tctggcgtgt
    38161 taccaacgcg aag
//
LOCUS       DAZUCE010000017        36471 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZUCE010000017 DAZUCE010000000
VERSION     DAZUCE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.017"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            236..1609
                     /gene="aroP"
                     /locus_tag="ACHY03_000822"
     CDS             236..1609
                     /gene="aroP"
                     /locus_tag="ACHY03_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFS3904091.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1652..2506)
                     /gene="ampE"
                     /locus_tag="ACHY03_000823"
     CDS             complement(1652..2506)
                     /gene="ampE"
                     /locus_tag="ACHY03_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFS3904092.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLHALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLTDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2503..3054)
                     /gene="ampD"
                     /locus_tag="ACHY03_000824"
     CDS             complement(2503..3054)
                     /gene="ampD"
                     /locus_tag="ACHY03_000824"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFS3904093.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWTRFRALVSKETT"
     gene            3142..4035
                     /gene="nadC"
                     /locus_tag="ACHY03_000825"
     CDS             3142..4035
                     /gene="nadC"
                     /locus_tag="ACHY03_000825"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFS3904094.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4238..4678
                     /gene="ppdD"
                     /locus_tag="ACHY03_000826"
     CDS             4238..4678
                     /gene="ppdD"
                     /locus_tag="ACHY03_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFS3904095.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4688..6073
                     /gene="gspE"
                     /locus_tag="ACHY03_000827"
     CDS             4688..6073
                     /gene="gspE"
                     /locus_tag="ACHY03_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFS3904096.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6063..7265
                     /gene="hofC"
                     /locus_tag="ACHY03_000828"
     CDS             6063..7265
                     /gene="hofC"
                     /locus_tag="ACHY03_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFS3904097.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQQDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7300..8343)
                     /gene="guaC"
                     /locus_tag="ACHY03_000829"
     CDS             complement(7300..8343)
                     /gene="guaC"
                     /locus_tag="ACHY03_000829"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFS3904098.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLCSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8499..8543)
                     /locus_tag="ACHY03_000830"
     CDS             complement(8499..8543)
                     /locus_tag="ACHY03_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904099.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8568..9188
                     /gene="coaE"
                     /locus_tag="ACHY03_000831"
     CDS             8568..9188
                     /gene="coaE"
                     /locus_tag="ACHY03_000831"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFS3904100.1"
                     /translation="MRFIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9188..9931
                     /gene="zapD"
                     /locus_tag="ACHY03_000832"
     CDS             9188..9931
                     /gene="zapD"
                     /locus_tag="ACHY03_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFS3904101.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9941..10138
                     /gene="yacG"
                     /locus_tag="ACHY03_000833"
     CDS             9941..10138
                     /gene="yacG"
                     /locus_tag="ACHY03_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFS3904102.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10460..10858)
                     /gene="mutT"
                     /locus_tag="ACHY03_000834"
     CDS             complement(10460..10858)
                     /gene="mutT"
                     /locus_tag="ACHY03_000834"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFS3904103.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRVYVG"
     gene            complement(10918..13623)
                     /gene="secA"
                     /locus_tag="ACHY03_000835"
     CDS             complement(10918..13623)
                     /gene="secA"
                     /locus_tag="ACHY03_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFS3904104.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDITGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13685..14197)
                     /gene="secM"
                     /locus_tag="ACHY03_000836"
     CDS             complement(13685..14197)
                     /gene="secM"
                     /locus_tag="ACHY03_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFS3904105.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14427..15344)
                     /gene="lpxC"
                     /locus_tag="ACHY03_000837"
     CDS             complement(14427..15344)
                     /gene="lpxC"
                     /locus_tag="ACHY03_000837"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFS3904106.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15445..16596)
                     /gene="ftsZ"
                     /locus_tag="ACHY03_000838"
     CDS             complement(15445..16596)
                     /gene="ftsZ"
                     /locus_tag="ACHY03_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS3904107.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16657..17919)
                     /gene="ftsA"
                     /locus_tag="ACHY03_000839"
     CDS             complement(16657..17919)
                     /gene="ftsA"
                     /locus_tag="ACHY03_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFS3904108.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17916..18746)
                     /gene="ftsQ"
                     /locus_tag="ACHY03_000840"
     CDS             complement(17916..18746)
                     /gene="ftsQ"
                     /locus_tag="ACHY03_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFS3904109.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18748..19668)
                     /gene="ddlB"
                     /locus_tag="ACHY03_000841"
     CDS             complement(18748..19668)
                     /gene="ddlB"
                     /locus_tag="ACHY03_000841"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS3904110.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19661..21136)
                     /gene="murC"
                     /locus_tag="ACHY03_000842"
     CDS             complement(19661..21136)
                     /gene="murC"
                     /locus_tag="ACHY03_000842"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFS3904111.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21190..22257)
                     /gene="murG"
                     /locus_tag="ACHY03_000843"
     CDS             complement(21190..22257)
                     /gene="murG"
                     /locus_tag="ACHY03_000843"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFS3904112.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22254..23498)
                     /gene="ftsW"
                     /locus_tag="ACHY03_000844"
     CDS             complement(22254..23498)
                     /gene="ftsW"
                     /locus_tag="ACHY03_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFS3904113.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23498..24814)
                     /gene="murD"
                     /locus_tag="ACHY03_000845"
     CDS             complement(23498..24814)
                     /gene="murD"
                     /locus_tag="ACHY03_000845"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFS3904114.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKISGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24817..25899)
                     /gene="mraY"
                     /locus_tag="ACHY03_000846"
     CDS             complement(24817..25899)
                     /gene="mraY"
                     /locus_tag="ACHY03_000846"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFS3904115.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25893..27251)
                     /gene="murF"
                     /locus_tag="ACHY03_000847"
     CDS             complement(25893..27251)
                     /gene="murF"
                     /locus_tag="ACHY03_000847"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFS3904116.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27248..28735)
                     /gene="murE"
                     /locus_tag="ACHY03_000848"
     CDS             complement(27248..28735)
                     /gene="murE"
                     /locus_tag="ACHY03_000848"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFS3904117.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEEHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28722..30488)
                     /gene="ftsI"
                     /locus_tag="ACHY03_000849"
     CDS             complement(28722..30488)
                     /gene="ftsI"
                     /locus_tag="ACHY03_000849"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFS3904118.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30504..30869)
                     /gene="ftsL"
                     /locus_tag="ACHY03_000850"
     CDS             complement(30504..30869)
                     /gene="ftsL"
                     /locus_tag="ACHY03_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFS3904119.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30866..31807)
                     /gene="rsmH"
                     /locus_tag="ACHY03_000851"
     CDS             complement(30866..31807)
                     /gene="rsmH"
                     /locus_tag="ACHY03_000851"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFS3904120.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31809..32267)
                     /gene="mraZ"
                     /locus_tag="ACHY03_000852"
     CDS             complement(31809..32267)
                     /gene="mraZ"
                     /locus_tag="ACHY03_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFS3904121.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32869..33873)
                     /gene="cra"
                     /locus_tag="ACHY03_000853"
     CDS             complement(32869..33873)
                     /gene="cra"
                     /locus_tag="ACHY03_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFS3904122.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVRASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33955..34041)
                     /locus_tag="ACHY03_000854"
     CDS             complement(33955..34041)
                     /locus_tag="ACHY03_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFS3904123.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34053..34544)
                     /gene="ilvN"
                     /locus_tag="ACHY03_000855"
     CDS             complement(34053..34544)
                     /gene="ilvN"
                     /locus_tag="ACHY03_000855"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFS3904124.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34547..36271)
                     /gene="ilvI"
                     /locus_tag="ACHY03_000856"
     CDS             complement(34547..36271)
                     /gene="ilvI"
                     /locus_tag="ACHY03_000856"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFS3904125.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRSNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(36213..>36471)
                     /locus_tag="ACHY03_000857"
     CDS             complement(36213..>36471)
                     /locus_tag="ACHY03_000857"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904126.1"
                     /translation="NCCKWWDLADLLSFLSFVMEDFIVSFHLSSCLFLLPGVYVSGCQ
                     LLKQDRTVNVFYTFFPSNSEAGHGDVVWSRDGRPIAYRSGR"
BASE COUNT         8712 a        10225 c         8983 g         8551 t
ORIGIN      
        1 aatgagaggg gttgaggctg agctttacaa acggtttctt tttaagcaac tcatcttcaa
       61 ccatgcataa agcgggtgca ttcgctgccg cataccatta ttcttgatct gacggaagtc
      121 tttttgtaac aattcaaact tctttgatgt aaacaaatta atacaacaaa cggaattgca
      181 aacttacaca cgcatcactg cgtagatcaa aaaaacaatc accgcacgag gtttcatgat
      241 ggaaggtcaa cagcacggcg agcagctaaa gcgcggcctt aaaaaccgcc atattcagct
      301 tatcgcgctg ggtggcgcga tagggacagg gttattcctg ggtagcgcct ccgtaataca
      361 gtccgcaggg ccagggatta tcctgggtta cgccattgct ggttttatcg cctttctgat
      421 catgcgtcag ctgggtgaaa tggtggtcga agaacctgtc gcaggctcct ttagccactt
      481 tgcttataaa tactggggca gctttgctgg cttcgcttct ggctggaact actgggtact
      541 gtacgtttta gttgccatgg cagagctgac tgctgtgggt aaatacattc agttctggta
      601 tccggaaatc ccaacctggg tttctgccgc cgtgttcttt gtggtgatta acgccatcaa
      661 cctgaccaac gtaaaagtgt ttggtgagat ggagttctgg tttgccatta tcaaagttat
      721 tgcggtagta gcgatgatca tcttcggcgg ctggctgctg ttcagtggta acggcggtcc
      781 gcaggcaagc gttagcaacc tgtgggatca gggtggtttc ctgccacacg gcttcaccgg
      841 gctggtgatg atgatggcga ttatcatgtt ctcgttcggt ggtctggaac tggtggggat
      901 caccgcagca gaagctgata acccggagca aagtatcccg aaagcgacta accaggttat
      961 ctaccgcatc ctgattttct atattggttc gttagccgtt ctgctctcac tgatgccgtg
     1021 gacccgcgtt accgccgata ccagtccgtt tgtgctgatc ttccacgagt taggcgatac
     1081 ctttgtggcg aatgcgctga acatcgtggt actgactgcg gcgctctccg tgtacaacag
     1141 ctgcgtatat tgcaacagcc gtatgctgtt tggtctggca caacagggta atgcgccaaa
     1201 agcgctggcg tctgtcgata aacgcggcgt accggttaac accattctgg tgtctgcgct
     1261 ggttacagca ttgtgcgtat tgattaacta tcttgctccg gaatccgcat ttggcctgtt
     1321 aatggcactg gtggtatccg cactggtgat caactgggcg atgatcagtc tggcgcatat
     1381 gaagttccgt cgcgccaagc aggaacaagg cgtggtaact cgcttccctg ctctgcttta
     1441 tccgctgggt aactggatct gcctgctgtt tatggcggcg gtactggtga ttatgctgat
     1501 gaccccagga atggcgattt cggtatacct gatcccggta tggctgatcg tgttaggtat
     1561 cggctatctg tttaaagaga aaaccgccaa agccgtaaaa gcgcattaat ctctctacgc
     1621 cctcacccgt acagggtgag ggcaataatc tttacaccaa cgccccgtaa atcgtcagta
     1681 gcgcaatcac caccacgacc acgaacgagg ttttcttcgc cattgaaacc gctgccttcg
     1741 gcgtctcaac cttatcgaca tgcggttcac gcgccagtga gaactgtgcc agacgcgtta
     1801 atacctggta ttgcgatgta tggaaatcgg tcagtgaagc aaaccaggct ggtaacgctt
     1861 tctcaccgtg accaatcaag gcataaacta cgcccgcaag gcgaactggt acccaatcca
     1921 gtacatgaag cacggcgtca atgccggact gtaaacgatg atgcggcgtc tgatatcgcg
     1981 ccagccagta ttgccatgca cgcaagaacg cataccccat cagcgtaacg ggtccccagg
     2041 ttccccccac aatcagccag aacagcggtg caagataaaa acgaaagtta atccacagca
     2101 atgcattttg cagctcacgc aaatactcac gttcgtcgca gcctgccggg acgccgtgaa
     2161 tcatggtgag ttcgccagcc atcgtggcac gggcatggct atcattacgt gaagctgctg
     2221 tcagataagc atgataatga agacgaactt tacctgcgcc aatacacagc aaaccaatca
     2281 gcagccacac cagcagcgtg gggacgttga acaatactcc ctgcaatgcg tgtaacagta
     2341 aaaaagtcac gcccatcgca ataatggtca tgcctaacgt gcgcccgaga gaaaaatgtt
     2401 tcacccgccg aaagaacgct tcaagacgat gatcaagctg ccagtgctcg cccaacttaa
     2461 acaggcgctc gaaaattaac accagtaagg ttgtaaatag cgtcatgttg tctccttgct
     2521 gaccagcgca cgaaaccgtg tccaatcaaa tgcagggccg ggatcggttt tccgatccgg
     2581 cgcaatatca caatggcccg tcatgttatt agcgatatcc ggatagcgat caatcagtgc
     2641 ccgcgtaacc gccgcaagct gttgatactg cgcatcggta tacgccagcg tatcggtgcc
     2701 ttcaagctca atcccaatag aaaaatcatt gcagcgttcg cgcccctgat actgagagac
     2761 tcccgcatgc caggcacgtt tatcgaaagg aacatactgg actatttcac catcacggcg
     2821 aatcaaacag tgagcggaga cgcgcaaatg ggcgatctca gcaaagaaag gatgtgcctg
     2881 cggatcaata gttccagtga ataatgcgtc gatccacgga ccgccaaact cgcctggcgg
     2941 caggctaata ttgtgcacca ccagcagggt gggtgtttcg tcgtccgggc ggcaatcgta
     3001 atgtggtgag ggaacgcggc gcgcgccaac cagccacccc tgttctaaca acatgcagga
     3061 tctccttata tgtggtgcta atacccggtt cagagtagca tgtttctacc ttatgattcg
     3121 ttagctatct ggagttttaa catgccgcct cgccgctata accctgacac ccgacgtgac
     3181 gagctgctgg aacgcattaa tctcgatatc cccggcgcgg tggcccaggc gctgcgggaa
     3241 gatttaggcg gaacagtcga tgccaacaat gacattacgg caaaactttt accggaaaat
     3301 tctcgctctc atgccacggt gatcacccgc gagaatggcg tattttgcgg caaacgctgg
     3361 gttgaagagg tgtttattca actggcaggc gacgatgtca ccataatctg gcatgtggat
     3421 gacggcgatg tcatcaacgc caatcaaccc ttgttcgaac ttgaaggccc atcccgcgtg
     3481 ctgttaacgg gcgaacgcac tgcgcttaat tttgtgcaaa ccctttcagg agttgccagt
     3541 aaggtacgcc actatgtcga attactggaa ggcaccaaca cgcagttgtt ggatacgcgt
     3601 aaaaccttac ccggtctgcg ttcagcgctg aaatacgcgg tactttgcgg cggcggagcg
     3661 aatcaccgtc tggggctttc tgatgccttc ctgatcaaag aaaaccatat tattgcctcc
     3721 ggctcagtgc gccaggcggt cgaaaaagcg tcctggctgc acccggatgc gccagtagaa
     3781 gtcgaagtag agaatctgga agaacttgat gaggccctga aagccggagc cgatatcatc
     3841 atgctggata acttcgaaac agaacagatg cgcgaagccg tcaaacgcac caacggcaag
     3901 gcgctactgg aagtgtctgg caacgtcact gacaaaacac tgcgcgaatt tgccgaaacg
     3961 ggcgtggact ttatctccgt cggtgcgcta actaaacacg tacaagcact cgacctttca
     4021 atgcgttttc gctaatagct gacagattta tccgctccat agtgggcgga taatctcccc
     4081 ttccccaacc ttcttcgtaa cgcctcgcaa atttcttgtc tttcgctgca acgaaataaa
     4141 aaacctcgga agcagctttc ccgtttcgtt ttttgcccct cgcctgtgct gatggcgttt
     4201 gccaaagtag caccaaccaa atcaaggagc gaaacagatg gacaagcaac gcggttttac
     4261 acttatcgaa ctgatggtgg ttattggcat cattgccatt ttaagcgcca ttggtattcc
     4321 cgcttatcaa aactacctgc gcaaagccgc actcaccgac atgctacaaa cctttgtgcc
     4381 ttaccgtacc gccgtagagt tgtgcgcgct ggaacatggt ggattagata cctgcgacgg
     4441 tggcagcaat ggcattccct cgcctaccac cacccgctat gtttcagcca tgagtgtggc
     4501 aaagggcgtg gtgtcgctga ccgggcaaga aagtctcaat gggctaagcg tcgtcatgac
     4561 gccaggttgg gataacgcaa acggcgtcac cggctgggcg cgcaactgca atattcaaag
     4621 tgacagcgca ttgcagcaag cctgcgaaga tgtcttccgc tttgatgacg ccaactaagg
     4681 agcggcaatg aatattccac agctcacggc cctgtgcctg cgttatcagg gagtcttgct
     4741 ggatgccagc gaagaagtgg ttcatgttgc ggtggtcgat gccccctcac atgagttgct
     4801 ggacgcattg catttcgcta ccaccaaacg tattgagatc acctgctgga cgcgccaaca
     4861 aatggaaggt cacgccagtc gcacacaaca gacattgccc gtagctgttc aggagaagca
     4921 tcagcccaaa gcagagttgc tgactcgaac gttacaatct gcgctggaac aacgcgcgtc
     4981 tgatattcat atcgaaccag cggacaatgc ctaccgcatc cgcttgcgta tcgacggcgt
     5041 attgcatcct ttaccggatg tttcaccgga tgccggagtc gcgttaaccg ccagattaaa
     5101 agtgctggga aacctggata ttgcggaaca tcgcctgccg caggatgggc aattcactgt
     5161 cgaactggca ggaaacgccg tctcatttcg tattgcgacc ttaccatgtc ggggtggtga
     5221 aaaggtggta ttaaggttgt tacagcaggt gagccaggca ctggatgtca acacgcttgg
     5281 aatgcagccg ttacaactgg cggactttgc tcatgccttg caacaaccac agggactggt
     5341 gctggtaact ggccctacag gcagcggcaa aacggtcacg ctttatagtg ccctgcaaac
     5401 gctgaatacc gctgacatta atatttgtag cgtcgaagat ccggttgaga tccccatagc
     5461 cggactaaac cagacgcaaa tccatccgcg tgccgggctc acctttcagg gcgttttgcg
     5521 tgcgttattg cgccaggatc ctgacgtcat catgatcgga gagatccgcg atggcgaaac
     5581 agcagagatc gctattaaag ccgcgcaaac cggtcacctg gtgttgtcta ccctacacac
     5641 caattccacc tgcgaaacgc tggtacgttt acagcaaatg ggagtcgccc gctggatgct
     5701 ctcatcagcg cttacgctgg taatagccca gcgtctggta cgtaaacttt gcccacattg
     5761 tcgccagcag caaggggagc ccatccatat tccagacaat gtatggccgt cgccgctgcc
     5821 ccactggcag gcacccggtt gtgtacattg ctaccacggt ttttatggtc gcacggcctt
     5881 atttgaagtt ctgcccataa caccggtcat acgtcagctt atttccgcta ataccgacgt
     5941 tgaatcgctg gaaactcacg cccgacaggc gggtatgcgt acgctttttg aaaacggctg
     6001 cctcgccgtg gagcaaggct taaccacctt tgaagagtta atccgcgtac tggggatgcc
     6061 gcatggcgag taagcaactc tggcgctggc atggcataac cggcgacggc aatgcgcaag
     6121 atgggatgct atgggcagag agccgtactt tactgcttat ggcactacag caacagatgg
     6181 ttaccccact aagcctgaag cgaatcgcca tcaattctgc gcagtggcga ggagataaaa
     6241 gcgcggaagt cattcatcaa ctggcgacgc tactcaaagc cgggttaacg ctttctgaag
     6301 ggctggcact gctggcggaa cagcatccca gtaagcaatg gcaagcgttg ctgcaatcgc
     6361 tggcgcacga tctcgaacag ggcattgcct tttccaatgc cttattaccc tggtcagagg
     6421 tatttccgcc gctctatcag gcgatgatcc gcacgggtga actgaccggt aagctggatg
     6481 aatgctgctt tgaactggcg cgtcagcaaa aagcccagcg tcagttgacc gacaaagtga
     6541 aatcagcgtt gcgttatccc atcatcattt tagcgatggc aatcatggtg gttgtggcaa
     6601 tgctgcattt tgttctgccg gagtttgccg ctatatataa gaccttcaac accccactac
     6661 cggcactaac gcaggggatc atgacgttgg cagactttag tggcgaatgg ggctggctgc
     6721 tggtgttgtt cggctttctg ctggcgatag ccaataagtt gctgatgcgc cgcccgacct
     6781 ggcttatcgc gcggcagaaa ttgctgttac gcatcccgat tatggggtca ctgatgcggg
     6841 gacaaaaact cacgcagatc tttacgattc tggcgctgac tcaaagtgca ggcattactt
     6901 ttttacaggg cgtagagagc gtcagagaaa caatgcgctg cccgtactgg gtgcaacttc
     6961 tgacacaaat ccagcaagat atcagtaacg gtcatcccat ctggctggcg ctaaaaaatg
     7021 ccggtgagtt tagcccgctc tgtttgcaat tagtgagaac gggagaggcc tccggctcgc
     7081 tggatctcat gttagacaac ctcgcccatc atcatcggga taacacaatg gcgctggcgg
     7141 ataacctagc agccttactg gaaccggcgt tgctgatcat aacgggagga attatcggta
     7201 cgctggtggt ggcgatgtat ctgccaattt tccatttagg cgatgcgatg agtgggatgg
     7261 gataacgctg gcgtgttgct ccacaccagc gttgggagat tacaggttgt tgaagatgcg
     7321 gttttcttgt tcctgcacac ggataaatgt ggtgcgcttg gtcagttctt tcaggcgtga
     7381 agccccaacg tatgtacaag ctgaacacag gccgcccaaa atatcacgcg cggtattttc
     7441 aaccgggcct cgcagcggca gcttaacggt tttaccttct gctgcgcgat attccgcaac
     7501 gccgccaacg tgacgtttca tcgcagactc ggagctcatg ccgtagaaca gcataaattt
     7561 ctcgccgttc tcctcaacga tgcgaccgcc gctctcttcg tggcccgcca gcatgccgcc
     7621 aagcatgacg aaatcggcac cgccgccaaa ggctttcgcc acatcgcccg gcgtggtgca
     7681 gccaccatcg ctgacgatca ttccgcccag accgtgcgca gcatcggcac attcgattac
     7741 cgcagaaagt tgcggataac cgacgcctgt tttgacacga gttgtacaaa cagaacctgg
     7801 gccaatgcca actttaacga tatcggcacc ggagaggata agctcctcac acatttcacc
     7861 agtcactacg ttgccagcac aaatggtttt ggtcggccac gcttcacgcg ctttcgcaac
     7921 gaactgcacg aagtgttcgg aataaccatt cgccacatca atacaaacga agtttaatgc
     7981 cgggttcagg tcgagaatct gtttagtttt ttcgaaatcc gcatcagacg taccggtaga
     8041 aaccatcaca tgtttcagca catcagcgga agaattgttg ataaacgctt gccactcttc
     8101 gacagaatag tgtttatgca cagcagtcaa aatatcaaaa gaagccagcg cagaggccat
     8161 agaaaatgtg cctacggtgt ccatatttgc ggcgataatc ggcacgccgg accagctctg
     8221 acctgaatgt ttgaaggtga attgacgttc cagttcaaca tcggaacggc ttttaagagt
     8281 ggagcgttta gggcggatga gaacgtcttt aaaacctaac ttcagatctt cttcaatacg
     8341 catgtgcgga ttcctggggt taatggctat aagggtaaga gcgcaactcc agtgacgcta
     8401 tcatacgcac taatcaatgc ggcgcaagac tgcgaaattg tcttttttta cgctacaatc
     8461 ccataaattt acctggtaaa tagtgagcga acgtttcatc aatttcgctt tcgtgcgctc
     8521 atttttaacg ttttgataat catgatgaat tcccgggaat ataaattatg aggtttatag
     8581 ttgccttaac gggaggcatt ggcagtggca agagtaccgt tgccaatgcg tttgctgatc
     8641 tcggaattaa cgtcattgat gccgatatta ttgcgcgtca ggtggttgaa ccaggtgcac
     8701 ctgcgctaca tgccattgct gatcactttg gcgctaacat gattgctgct gatggaacat
     8761 tgcagcgccg ggccttgcgc gagcggatct tcgccaaccc ggaagagaaa aactggctta
     8821 acgccctgct gcatccgctg attcaacaag agacgcaaca ccagatccag caagcaactt
     8881 ccccctatgt actgtgggtt gtgccattgc tggtagaaaa ctcactgtat aaaaaagcga
     8941 atcgagtgct ggtggtggat gtcagcccag aaacgcaact taagcgcacc atgcagcgcg
     9001 atgatgtaac tcgcgagcat gtcgaacaaa tccttgctgc tcaggcaacg cgcgaagccc
     9061 gccttgccgt ggcagatgac gtcattgata ataacggcgc accggatgct atcgcatcgg
     9121 atgttgcccg cctgcacgca cactatttgc agcttgcgtc gcagtttgtc tcacaggaaa
     9181 aaccgtaatg cagacccagg tcctttttga acatccacta aatgaaaaaa tgcgtacatg
     9241 gctgcgcatt gagtttttga ttcagcaact caccgttaat ttacccatcg ttgaccacgc
     9301 tggcgcgctg catttcttcc gtaatgtcag tgaattactg gatgttttcg agcgcggcga
     9361 agtccgcact gagctgttga aagaacttga ccggcagcaa cgtaaactcc agacctggat
     9421 tggcgtgcct ggcgtggacc agagccgtat tgaagcatta attcagcagt taaaagcggc
     9481 ggggagcgta ttaatttccg cgccgcgtat cgggcaattt ctgcgtgaag atcgtttgat
     9541 tgctctggtg cgtcagcgac tgagcatccc aggcggctgt tgcagctttg atttaccaac
     9601 attgcacatt tggctgcatc taccccaggc gcagcgcgac agccaggtag aaacctggat
     9661 tgccagcctg aacccgctca cccaggcact taccatggtg ctggatttaa ttcgccagtc
     9721 ggcccccttc cgtaaacaaa ccagcctgaa tggtttttat caggataacg gtggcgatgc
     9781 cgacttgctg cgcctgaatc tgtcgctcga ttcacagctt tatccgcaaa tttccggaca
     9841 taagagccgt tttgccattc gttttatgcc gctggacact gaaaacgggc aggtaccgga
     9901 acgtctggat ttcgaactgg cctgttgcta aggagtaaaa atgtcagaaa ctattacggt
     9961 gaattgccca acctgcggga aaacggtggt gtggggtgaa atcagcccgt ttcggccatt
    10021 ttgctccaaa cgttgtcagc tgatcgacct cggagaatgg gctgctgaag aaaaacgaat
    10081 ccccagcagt ggcgatcttt ccgaaagcga tgactggagc gaagaaccaa agcagtgaca
    10141 tttgtctgat gccgcacgta ggcctgataa gacgcggaca gcgtcgcatc aggcatcttg
    10201 tgcaaatgtc ggatgcggcg tgaacgcctt atccgaccta caaaaacaaa tttatcaatg
    10261 agttagaaat ccacgtaggc ctgataagac gcgccagcgt cgcatcaggc atcgtgtgcg
    10321 aatgtcggat gcggcgtaaa cgccttatcc gacctacaaa aacaaattta tcaatgagtt
    10381 agaaatccac gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg tgtgcaaatg
    10441 tcggatgcgg cgtgaacgcc tatccgacat acacacgctt aagcttcgca attaccggtt
    10501 cattggctgg cggaaaatca tcggcattaa gaccgactag cgacatccac tcaccgggtt
    10561 gcccttcttt accccacggc tccccttccc agctttcgac cagccagaac cacagagtta
    10621 tatgcctgtc cgggaattca tattccagtt tttcaaatag cgaaaaatgt tgtggggtaa
    10681 tcccgacttc ttcctgaagt tcacgcacca ccgcctgttc cggcgtttca cccatttcaa
    10741 ttttaccgcc gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgcgtta
    10801 taaagatttc attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg
    10861 tcttaaacct ataaaaaagg cgcagaatcc tgcgcctttt acttcaacag ttagctttta
    10921 ttgcaggcgg ccatggcact gcttgtattt tttaccagaa ccgcacgggc aaggatcgtt
    10981 acgtcctact ttgcgctcac cggtttgcgc cgccagtgca gctgcggctg cagagtcgtc
    11041 atcctgatgg ctaagctgct gcatttgcgc taaacgctcg gcttccatac gacgctgttg
    11101 ttccagctcc tcaacctctt caggcatacg tacctgaact ttgctcagcg tgctgataac
    11161 ttcatatttc aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc
    11221 ctgcttcgga tctttctgtg cgtagccacg caggtggata ccctgacgca gatagtccat
    11281 cgctgccagg tgctctttcc acagggagtc aagcgtttgc agcatgacgc ctttctcgaa
    11341 gtgacgcatc atctctgcac caaccacttc ttctttacgc tgatacactt cgatggactg
    11401 cgccagaatg cgctcacgca gcgtctcttc atgcagttct ggttctttat ccagccactc
    11461 agcaattggt aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccgtaatatc
    11521 ccacatttct tccagcgact gtggcggaat gtaggcatca atggtggctt tgaacacatc
    11581 ttcacgaatg ctgttaatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg
    11641 ggagtaaatg gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg
    11701 aatgtcgaag ttacggcttt caactttacg ctgggcgtta gcaatcgctt tggtcaccca
    11761 cgggtgttca atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg
    11821 gtcggaagca aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc
    11881 agcatccccc tgacgaccag aacgaccgcg caactggtta tcgatacgac gggattcgtg
    11941 acgctcggta ccgatgatat gcaggccacc tgctgccagt accgcatcgt gacgtacctg
    12001 ccagtcggct ttaatttttt caatttgctc tgcggtcgga ttttccagcg cggcaacttc
    12061 tgcctgccag ctaccaccga gcacaatatc tgtaccacga cccgccatgt tggtcgcgat
    12121 agtcaccgca gccggataac ctgcctgagc aacaatcgcc gcttcgttgg cgtggaattt
    12181 ggcgttcagg acattgtgct taataccggc tttggtcagt tcgtttgaca ccagctccga
    12241 tttttcgatg gagatagtac ccaccagcac cggctggcct ttcgcagtac gttctttgat
    12301 atcttcaatg atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt ccggcagatc
    12361 tttacgaatc attggacggt tggtcggaac aacgacggta tccagtttgt agatggagct
    12421 aaattcgaaa gcttcggtat cagcagtacc ggtcatcccc gccagttttt catacagacg
    12481 gaagtagttc tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc
    12541 ttctttcgct tccacagcct ggtgcagacc atcggaccag cgacggccct gcatggtacg
    12601 accggtgtgt tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcacg
    12661 ggtaaacagc gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc
    12721 cggagagtac agagactccc cttcatccat gatgccctct ttcaccagca gttcttcaat
    12781 caggaccaga ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca ccgagaagtg
    12841 gccttcgccc tggaaggttt cggagtcttc tttttcctgg cggatcaggt gtggaataat
    12901 tttattcacg cgtttataca tttccgagct gtcttctgcc ggaccggaaa tgatcagcgg
    12961 tgtacgcgct tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt
    13021 acgctgtaca cgttcttcag ggctgaacgc catattgtcg cgcaggtagt caaagccgta
    13081 ttcgttgttc gtaccgtaag tgatgtcagc tgcgtaagct tcgcgctttg ccggtgctgg
    13141 catgcccggc aggttgatac cgacagtcag gccaaggaat tcaaacagcg gacggttgtt
    13201 ttcggcgtca cgttgcgcca ggtagtcgtt gacggtaact acatgcacgc ctttaccggt
    13261 tagtgcgttc aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat
    13321 ttcggcgatg cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg
    13381 cataccaaaa acgcgcttac ttgcctcacg caccacggcg aaagcttccg ggatcagatt
    13441 ttccagcact tcgccttttt ccagacgcgc acgaaactct gcggttttcc ctttcagttc
    13501 ttcgtcggag agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg
    13561 catccggcgc agggtgcgat cgttacgact accgaaaact ttagttaata atttgattag
    13621 cataataaaa tctcaaacgc cccgcgttgc ggagttaata aaatgaagta aaggtttatt
    13681 gttgttagct gaggcgttga gggccagcgc ggatgccttg cgcctggctt atccagacgg
    13741 gcgtgctgaa ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa cccttttcag
    13801 acggcgtgcc ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca agatgttgcg
    13861 cctgaagagg caaagattct tcagcaacgg gcagcgtttg cggtgccatt gcgaaggaaa
    13921 gatgacggat taccgtgcga atggcatgtt gatgccagta atcaacggaa tagttcgaat
    13981 tcgggcggcg cgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag
    14041 gctcgtggtt gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg
    14101 caggcaaacc taaactcgcc gcaaccatcc ctaataagag atgcggccag aagtagcgtt
    14161 taccaaactg tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcgtatt
    14221 caaacgtatt tagcgcacaa atcttatcat taaagcctga agtccacagc acgaatgaag
    14281 ataagttgtg cgtaaaagtg cttaagaaaa cagcgttcgc accgtttttt atagctctta
    14341 taaaattcaa aaaaaacgac tggttcacct ggccggagag agtgccagat ttgccagtcg
    14401 aattttatac gacagtataa atgtcgttat gccagtacgg ctgaaggcgc tttgaaggcc
    14461 aacggcagtt ctgcgtcgtc ctggaaggtc acatattccc aggcttcctg tttcgccagg
    14521 acagcctgca gcagtttgtt attcagtgca tgaccggatt tataagcggt aaatgcacca
    14581 ataatattgt gaccacacat gaacaagtca ccgatcgcat cgagcatttt gtgacgcaca
    14641 aattcgtctt caaaacgcag gccgtcttcg ttcagtacgc gataatcgtc aacaacgatg
    14701 gcacaatcga agctgccgcc caggcacaaa ccacgggact gcagatattc gatatcacgc
    14761 atgaaaccga acgtacgcgc acggctgatc tggcgcataa acgcatcagc ggagaagttc
    14821 atcgcatagc gctggttgct ggaatcaata gccggatggt taaaatcgat ggtgaaatcc
    14881 agcgaaaaac cattgtacgg cttaaattca gcccacttat cgccatcttc gacacgaaca
    14941 gtctctttga tgcgaacaaa tttcttggcg cagttcaact cgtcgatacc ggcgtcaagc
    15001 agcaggtata caaacggagc ggcgctgccg tccatgatcg ggatttccgg cgcgttaact
    15061 tcgataacaa tgttatcgat gcccaggccc gcgagagcag cattgaggtg ctctacggtt
    15121 gaaatccgta catcatgctc gttgaccaga cacgtacaga gcatggtatc acgcacagat
    15181 ttggcatcgg ccgggaaatc taccggtgga ttcaagtcgg tgcgacgata gatgaccccg
    15241 gtgttggccg gcgcagggcg taacgtcagg gtgactttct tgccggtatg taaaccgaca
    15301 cccgtcgcct gaacgatacg tttaagtgtc ctttgtttga tcatcgtatt atctcgccaa
    15361 attacctatc caaccgaagt gtactataca ttcggcgggc cagtttagca caaagagcct
    15421 cgaaacccaa attccagtca attcttaatc agcttgctta cgcaggaatg ctgggatatc
    15481 cagataatcc ggctctttcg cagtttgcgg cgcattgtca ttcacgactt tagcaaccgg
    15541 cttctgctcc tgggtcagcg gagccatccc atgctgctgg tagcgatcca tcactggctg
    15601 ctgaacctgc ttattggtca ccagagtgat ttcaggacgt ttgtccatgc cgatacctgt
    15661 cgcaacaacg gttacgcgca gctcgtcatt catatccggg tcaagagaag taccgataac
    15721 cacggtcgcg ttgtcggaag caaatgcacg gatggtgtta cctaccgttt cgaactcatc
    15781 cagacgcagg tcgaagcccg ctgtgatgtt aaccagcacg ccgcgtgcgc cagacaggtc
    15841 gatatcttcc agcagcggag aagagatagc catttcagca gcttcttccg cacggtcttc
    15901 accgcttgcc acgccagaac ccatcattgc gtagcccatt tcagacatta cggtgcgtac
    15961 gtctgcaaag tccacgttca tcaaacccgg acgagtaatc agttcggcga taccttgcac
    16021 agcgcctttc agtacatcgt tcgctgcgcc aaacgcatcc agcagggaga taccgcggcc
    16081 cagaactttc agcagtttgt cgttcgggat agtgatcaga gagtccacat gcttggacag
    16141 ttcagtgatc ccctgctcgg cgaatgccat acgcttcttg ccttcaaagt tgaaaggctt
    16201 agtgacgaca gcaacggtca ggatacccaa atcttttgcc acttcagcga cgactggtgc
    16261 tgcacctgta ccggtaccac cacccatacc cgcagcaata aagaccatgt ctgcaccttc
    16321 cagcgccgca cgcagtgcat cgcgatcctc atcagccgca ttgcggccaa cttctggatt
    16381 agcgcctgcg cccagtcctt tggtgatacc gctaccgatt tgaatcgtct gtccaaccgc
    16441 tgttttacgc agcgcttgtg catcggtatt taccgcgaag aattcaacac cttcaatgcg
    16501 ctcgcgcacc atgtgttcaa cagcattacc gccgccgccg ccgacgccga tgactttaat
    16561 caccgcgtca ttggtaagtt ccattggttc aaacatagtt tctctccgat ttgtgcctgt
    16621 cgcctgaggc cgtaatcatc gtcggcctca taaaaattaa aactcttttc gcagccaact
    16681 attgagtcgc ttgatccacg agccaactga tgctgtaaca cgtttttcta cttcagcttc
    16741 accgttaaga tgtgactctt tcccatagtg aagcaatccc accgccgtcg aataatacgg
    16801 ctcctgagca taatccgtta aaccggtaat gttcagcggc gcgccgatac gcacttgcgt
    16861 atgaaacacg cgctgagcac aggctgcaag accttcgatc tgcgctgcgc caccggttaa
    16921 tacaatgcct gccgccaggt gatgtttaac cccttgttgg cgaagctttt cctgcaactg
    16981 caatatctct tcgttgacca ggttgagcag ctcggtatag cgcggctcga tcacctctgc
    17041 cagggtctga cgttgcagac tccgcggcgg acgaccgcct acgctcggca cttccacgct
    17101 ctcatctttt ccaacgatgg aacccagcgc acaaccgtgg cgaactttaa tcgcttcggc
    17161 gtcgcttggc ggcgtgccaa aggcgtaagc gatatcactg gtcacgacat tgccagcata
    17221 aggaattacc ttagtgtggc gcaatgcccc accggtataa acggcgatat ccattgtacc
    17281 accaccgata tcgacgacgc agacacccag ttcacgttca tcttccgtca ataccgaata
    17341 acttgatgcc agaccggcaa atatcagttg gtcaactttc agcccacaac gttcaaccgc
    17401 tttgacgatg tttttcgcca tatcgttgtg acatgtgatc aggtgcactt ttgcctgcat
    17461 ccgcacgccc gaaagtccta ccggattctt gatcccttcc tgatagtcaa tcgcatactc
    17521 ttgcgggatc acatgcagca cacgatgctc atcgcgcaca cgcaccgatt tcgcggtatg
    17581 gacgacgttt tccacatctt cttgcgtcac ttcttcttca gaaataggca ccataccaat
    17641 ttcattctgg cagctgatgt gcttaccaga aagcgccaga tataccgaag agatctgaca
    17701 atctgccatc aattctgcct ggtcaatggc acgttgtacg cacttgacca cggattcgag
    17761 gtcgttcacc ccgcctttat ccataccacg cgacgggcag ctgcccacgc caatgatatt
    17821 gaccataccg tcgggcagaa cttcccctac taaagcggca accttcgcgg taccaatctc
    17881 cagtcctact accagttttc tgtccgtcgc cttgatcatt gttgttctgc ctgtgcctga
    17941 ttttgttgct gagtagattc ctctggcggc aagggcgccc agcctactgc cgctccagag
    18001 tcataacgca aatcaacgta gctaatccgt ttgccatcgg tttgcgcctg ctgctgtaaa
    18061 accggataaa gttctacaaa gcgagccaaa cgtttcatcg tatcgccccg gccaagattg
    18121 agcttaatat cgttattcag cgtcaactgc caggaacgcc gcgcggtcat cgccgcttcc
    18181 ttcagagtaa atctgtcctt tgccagcatc tgccccattt cgcgatagcc ctgcaacact
    18241 tcattggcgc tgccttccgg gccatacagc attggaagca cctgcttgct ggtgcgatct
    18301 ggcggcacgc tgaaggtatt tccttccgcg tctaccatgt gttgatcatt ccaccgcgca
    18361 atcggcacat attcaaccag atgaatcttc aattcatcag gccactgctt tctgacgctc
    18421 acctgcttaa tccacggcag gcgttgttct atttgcgtct ggatgatgtt gacatcctgg
    18481 gtcataaagg tacccggctc acccaatgcc aggatcgact gccggatatc gtcattacgc
    18541 gtgtaatggc gttcaccggt caacaccagc tttgagagcg gcaggcgttg cgcatcttcc
    18601 atccagccca acacgaccca gccgctcacc aacactgtcg ttaaaacggt cagcaggaaa
    18661 aggatccccg ccagacgcgt tccattattg cggcgagaag aaacctcttc ttcgctgttt
    18721 cgcgtgttca gagcagcctg cgacatatta gtccgccagt tccagaattc gtactaccaa
    18781 ctgcgagaag ctcatacctg cctgacgtgc cgccatcggc accaggctgt ggctggtcat
    18841 acccggtgag gtattggctt ccagcagata aaactgtcca tcgctgtcca gcataacgtc
    18901 aatacgtccc catcctttgc aacctaacgt cgtccatgct ttcagcacta atgcctgcaa
    18961 attggcctct tgtgacgctt ccagaccagc ggggcagaaa tactgtgtct catcagagag
    19021 atacttcgcc tcataatcat agaaggttcc ggccggttga atacgtattg acggtaaaat
    19081 ttcttcaccg agtatcgcaa ccgtgaactc cggcccactt agccattttt caatcaatac
    19141 ttcttcatcg tgctgaaatg ccaatcttaa tgcatcttgt agagcatttt ctgctactac
    19201 ttttgacatt cccacactgg aaccttcgcg gctcggctta acgataaccg gcaaacccag
    19261 agcagaaatt tctgctaact gcttatcgct aaggcctttt tcaaactctg cgcgggttaa
    19321 cgctacccac ggcgcgaccg gtaaaccagc accttgccac agcagtttgc tgcgtagttt
    19381 atccattgaa agcgcagatg ccatcactcc gctgccggta taaggcaagc ccatcagctc
    19441 gagcatgccc tgcagcgtac catcttcacc gccgcgaccg tgtagcgcga taaacacttt
    19501 ctgaaagccc atcgacttta gttgcgtcac gtcgacttct ttcgggtcga caggatacgc
    19561 atcaataccg ccttcacgca gcccggctaa caccgctgcg ccagaattca gagaaacttc
    19621 ccgctcagcg gaagtcccgc ccaacaggac cgcgatttta tcagtcatgt tgttcttcct
    19681 ccggagtttg cggcttcaat ttgatttcag ctaaagaacg ggcaattttt ccgatattac
    19741 cagccccctg aacgagaatc aggtcgttac cggttaatac cggtgccagc atctcggcta
    19801 cctgcgcagg gtccggcacc agaatgggat caattttccc acgtccacga attgtgcgac
    19861 acagcgaacg gctgtccgct cccggaattg gcgcttcgcc agccggatac acttccagca
    19921 tcaacagggt atcaacctgc gtcagcacat tggcgaaatc atcatacagg tcgcgcgtac
    19981 gggtaaaacg gtgcggctga aacagcatta ccaggttttt atccggccag cctgcgcgcg
    20041 ccgctttaat agtggcgtcc acttccgtcg ggtggtggcc gtagtcatcg accagcattg
    20101 ccgtaccgct tttaccattc actggctcca gcgggaattc accgaggaaa tcgaaacggc
    20161 gccccgtccc ctggaagctc tccagcgcac gcaaaatagc ctcgtcgtca attccctctt
    20221 ccgtagcaac cgcaaccgca gctgcggcgt tcagcgcgtt atgacgacct ggcgcattca
    20281 gggtgacgcg catcggctct ttgtcctggc gcagcagcgt aaagtgcccc tgcgggccaa
    20341 tctgctgata atcttctaca cgcacgtcgg catcttcgct gaagccataa gttgtggtct
    20401 gacgtcccac acgcggtaac aattcgcgga tcaccggatc atcaacacac atcaccgcac
    20461 gaccgtaaaa cggcaggttg tgcagaaaat taataaaagt ctgttttaaa ttctcaaagt
    20521 cgccctggta ggtatccatg tggtcggctt cgatattggt gacaatcgcc accatcggtt
    20581 gcagatgcag gaacgatgca tcactctcat ctgcttcggc aatcaggtac cgaccatgcc
    20641 ccaaacgcgc atgaaccccc gccgctttta ccagcccgcc gttaacgaag gttgggtcga
    20701 gccccgcttc tgcgtagatg ctggagacca tcgcggtagt tgtcgttttg ccgtgcgttc
    20761 ctgcaatggc gatgccatga cgaaaacgca ttaactcagc cagcatttcg gcacgacgga
    20821 tcaccggaat acgcgcttca tgagctgcga caatttccgg gttatcggca gaaatggcgc
    20881 tggaaacaac gaccacgctg gcatcacgta cgttttccgg gcgatggttg aaataaatcg
    20941 tcgctcccag attcattaac tgctgcgtga ccggatttgg cgctaaatcg gaaccactga
    21001 tctgataacc ttcattggcc agaacttcgg caataccacc cataccggca ccaccgatgc
    21061 cgacaaaatg tatgtgccga acgcgacgca tttcgggcac gatggaacgc agttttgcca
    21121 attgttgtgt attcattctt tacgccatta acttcttaaa ttcatacgat gcaaaaggca
    21181 tcgctacaat tacgcccggg cagcccggct cacttcattt gccactcgct cggtggcatc
    21241 cggaatggat gcagcgcggg cgcgttctgc catggttaat aaggtttctc gcgaccaccc
    21301 ggccagggtg ttggcgacag catccacgct aagctgtggc tgctcgataa ttttggctgc
    21361 gcccgctttt tccagcggta gcgcattcca gtattgctgg cggtctttat gttgaaacgg
    21421 cacaaacaac gccggtagtc ctgccgcggc gatttcactc accgttaacg caccggagcg
    21481 gcaaacgacg acatccgccc acgcatacgc cgccgccata tcatcaataa attccgtcac
    21541 tttatgctgc ggttgccccg cttcggcata cgcctgttca acggattgtt gcgaaccttt
    21601 gccgctctga tgccagatag tgactgaatc acccagtttc gcagcaacct gcggcattgt
    21661 ctggttaaga atgcgtgcgc cctgggaacc acccactacc agcacacgaa ccggaccttc
    21721 acgtccagcc aaacgttgct gcggcaacgg tagcgccaac acatcggtac gcaccgggtt
    21781 acccaccact tccgcattgg ggaaagcacc tggaaacgcc tgcatcactt tggtggcaat
    21841 cttcgccagc catttattgg ttaagcccgc aataccgttt tgttcatgaa gtacaaccgg
    21901 aatgcctaac gaccacgcgg ccagaccacc aggacctgat acgtagccgc ccataccgag
    21961 caccacgtca ggtttgtacg ctttcataat cgcccgcgcc tgacgccagg cattgaagat
    22021 acgcagcggc gcagctatca gtgcttttat accttttcca cgcagaccag agatacgaat
    22081 gaaatcaatt tcgatgccat gttttggcac taagtccgct tccatacggt cggcagtccc
    22141 cagccagcga acttgccaac cctgagccat tagatggtgc gcaaccgcca gtcccgggaa
    22201 tacatgtcca ccggttccgc ctgccatcac cattaatcgc tttccttgac cactcatcgt
    22261 gaacctcgta caaacgcctg cgctttctcc agacgcgttt cataatcaat acgcaacagc
    22321 atcatgatgg ctgtcgacat aatcagtaag ctcgaaccac cgtaactgat cagcggcaat
    22381 gtcagacctt tggtcggtaa catccctgcc gccgcgccta cgttaaccag cgcctggaag
    22441 ctaaaccaga tgccaatgga acaggcgaga aaaccggaaa aacggtggtc aatttctaac
    22501 gctttgcggc caatcgacat cgcgcgaaaa gcgacgaaga ataccattaa aagtgccagc
    22561 accacaccga cataccccag ttcttcgccg ataatggcga aaataaagtc agtgtgcgct
    22621 tccggcagat actccagttt ttgtaccgag ttacctaaac cttgccccca aagttcgccg
    22681 cgaccaaacg ccatcagcga ttgcgttaac tgatagccgc tgccaaaggg atcttcccac
    22741 gggttccaga atgcggtaac acggcggata cggtacggtt cggcgaggat cagcaacaca
    22801 accgctgaaa tgcccatacc gataatggca atgaactgcc acaatttcgc tcccgcaagg
    22861 aacaacatcg ccagcgtagt cacaaacaac accaccaccg taccaaggtc tggctgtgcc
    22921 agcagtaaca ctgccaacac cagaatcacg cccatcggtt tcaggaagcc gcgcaggtta
    22981 ttacgtactt cgtcaccttt acgcaccaga tagttggcga tatagcaaaa cagcgacagt
    23041 tttgtcagct ccgcaggctg gatacgcagc aaaccgagat cgatccaacg cgatgcccct
    23101 ttaacagagc tacccactac caacacaatc atcagcagga tgatagatcc gagcagcatc
    23161 gtggcactgt agcgttgcca gaactccatc ggcagacgca gcgtaatgat cgccagaatg
    23221 aatgccagga tcagatagac gccatcacgc ttcgcgaaga agaacggatc gttggttaag
    23281 cgttgcccta tgggcattga cgccgaggtc accatgataa agccaatcgc cgcgaggccg
    23341 aaggtcagcc acagtaaggt gcgatcgtac atgatcaggc tgtcggtatc tttttcccgc
    23401 gagcccatca cccagccctt tagcgccgtg gagatccaga ccaggatact gaatcctggc
    23461 aggcgcggca ttttcaggcg agggagagat aaacgcatca acctaactcc ttcgccagac
    23521 gggcaaactc attgcctcgt tgttcaaagt tcttgaactg atcaaggctg gcacaggccg
    23581 gggagagcag aaccatatct cctggttgaa cacgcggagc cagcaagcgc atcgcctgtt
    23641 ccatagtttc ggtttgttct gccacttccg ggcgtaacgc cgccagctgc gcgccgtcac
    23701 gaccgaaaca atacagacgt acgttatcgc cattcaggta acgcgccagt gggctaaagt
    23761 ccgccgattt accatcgccg cccagcaaca aatgcagcgt gccgtctacg tgcaggccat
    23821 tcagcgccgc ttccgtactg ccgacgttgg tcgcttttga atcgttaatc caacgtacgc
    23881 cgttatgctc cagcacaact tcaaagcgat gcggcagacc agtgaatgtg gttaacgctt
    23941 tcaggctgct ggctcgcggt aaccctgcgg catctgccag tgccagcgcc gccagcgcat
    24001 tggtgtagtt atgctgcccg gaaattttca tttctttcac gttcagcact ttctcgcctt
    24061 tcacccgcag ccaggtttcg ccctgctgat gattcaggtg atagtcaccc atgttgacgc
    24121 caaagctgac gcagcgttca tccgcaccgc gaatcggcat tgttaaggca tcatcagcat
    24181 taaccacgca aactttcgcg ttttcgtaaa tgcgcagttt tgctgcacga tactgttgta
    24241 aaccaaacgg atagcgatcc atatgatctt cagtcacgtt cagaatggtc gctgctaccg
    24301 cctgtaagct ggaggtggtt tccagctgga agctcgacag ttccagcacg tacagttcac
    24361 actcatcatc cagtagcatc aacgcaggca ggccaatatt gccacccaca ccaacgttaa
    24421 cccccgccgc tttcgccatt tcacccacta gcgtggtgac cgtgcttttg ccgttagaac
    24481 cggtaatcgc cacaatcggt gcttgtgctt cgcgacagaa cagctcgata tcgccaacga
    24541 tttcgattcc ggcatcagcg gcagcgctta aggatggatg cgccagtgca ataccgggac
    24601 tggcgacaat cagatctgcc gccatcagcc attcatcatt cagactgccc gtgtggcgtt
    24661 caacggcttc gggtaattta tccaggccag gcggtgtcat acgcgtatcc ataacgcgcg
    24721 gcgtcacacc gcgagcgagg aaaaagtcca cgcaggaaag cccggtgagg cccaggccga
    24781 taatgacaac atttttaccc tggtaatcag ccatgattaa cgtaccttca gcgttgccag
    24841 accaatcagg accagcatca gcgaaataat ccagaaacgc acaatgacgc gcggttccgg
    24901 ccagcctttc agttcatagt ggtgatgaat cggtgccatg cggaaaatac gttgtccgcg
    24961 cagtttaaag gagccgacct gcaggatgac agaaagcgtt tctaccacga acacgccccc
    25021 cataatcacc agcaggaatt cctgacgtag cagtacggcg ataatgccta acgcaccacc
    25081 taacgccagc gaacctacat cgcccataaa gacctgcgcc ggataggtgt taaaccacag
    25141 gaagcccagt cctgccccga ctatcgcggt acagacaata accagttccc cggcgtgtcg
    25201 cagatacggt atgtgtaaat agctggcaaa gttcatgttg ccggtcgccc acgccaccag
    25261 cgcaaaacca ccggcgacaa atacggtcgg cataattgcc agaccgtcaa gaccatcggt
    25321 caggtttacc gcgttgccag tacccacaat gacgaagtaa gccagcagaa tgtagaacag
    25381 ccccagttgc ggcatcacat ctttaaagaa tgggaccacc agctgcgttg cgggcgtgtc
    25441 tttgccaaca aggtacagcg cgaaggcgac acccagcgca atgaccgaca tccagaaata
    25501 cttccaacga gcgatcaacc ctttggtgtc tttacgcact actttgcgat aatcatcaac
    25561 aaagccaata acaccgtaac ctaccagcac caccaacacg caccagacgt acggattgga
    25621 cgggtaagcc cacagcagta cggagatcac aatcgccgtc aggatcataa tcccgcccat
    25681 ggtcggcgta ccgcgcttgc tgaagtgtga ttcaggaccg tcgttacgca ccacctgacc
    25741 aaaggagagt ttttgcaaat gagcaatcat gcgcgggccc atccacaatg agatgaacag
    25801 cgcggtcagc aggctgacga tggcgcgaaa cgtcagatag gaaaagacgt taaagccgga
    25861 ataatatttg accaaatgtt cggccagcca aactaacatg tcccattctc ctgtaaagcg
    25921 cgtactacct cttccatggc ggcactacgt gaacccttaa ctaaaatcgt aattacctgt
    25981 tgctcagcaa tcagtgattt aagacgcgta attaacgcag ttttatcagc aaaatgttcg
    26041 ccaacgccgc tggcggtgct gatagcatgg ctttgtttac ccacgcttaa cacgcggtca
    26101 ataccagcag cttttgccgc ctcgcccacc tgtacatggc aggcttcgct ttcagcgccc
    26161 agttccgcca tatcgcccac caccagcacg cggtagcccg gcatttcagc cagtacctgg
    26221 actgctgcgg tcattgaacc gacattggcg ttgtaggagt cgtcgagcag caactggttt
    26281 tctgccagtt ggatggggaa cagacggcct ggaacagctt tcagatttgc cagccccgct
    26341 ttgatagcat caagcgttgc gcccacggac atggagagcg cagcggctgc cagcgcattc
    26401 gcaatattgt gacgccccgg caacggcagc agaacatcca cgctaccggt tggggtttgc
    26461 agggtaaatt ccgtaccgtg cgaagtcaca tggatattgg tggcggtgaa atcgctgttg
    26521 gcggcattag gtgagaaacg ccacactttg cgtgagccaa ttacgctctg ccaattcagc
    26581 cagtcgttgt tgtcggcgtt cataatggcg ataccgtttt ccggcaggcc gctaaagatt
    26641 tcacctttcg ctttcgcgac acccgcaagc gagccaaaac cttccagatg cgccgctgcc
    26701 aggttgttga ccagcgcagc ttccgggcga gtcagactca cagtccaggc aatttcgccc
    26761 tgatggttcg cgccaagttc aataactgcg taatcgtatt ccggcgttaa gcgcaacagc
    26821 gtcatcggta caccgatgtc gttgttgaga ttgcctgccg tataaagcgt gttgccgcac
    26881 tggcttaaaa tcgccgccgt catctcttta acggaggttt tgccggagga ccccgtcaga
    26941 gcaaccacgc gcgccggaac ttgctggcga acccatgcag ccagttcacc aaacgccaga
    27001 cgcgtatcct tgacgattaa ctgcggcagg tcgatatcca gcggacggct aaccagtagt
    27061 gcgcctgcgc cgccagcttt cgcctggtcg gcaaaatcat gagcatcgaa acgttcgcct
    27121 ttcagggcaa caaacaggca gcccggcgtc agttttcggg tatcagtggt tacagcatca
    27181 agggtgatat ctgcaccttg cagttcaccg ttgagaatgt cggtaagttg gctaagggtt
    27241 acgctaatca tgcaatcacc cccagcagac gcgccaccgt gacgcgatcg gagtagtcca
    27301 gacgctgatt gccaacaatc tggtaatctt catggccttt gcccgcgacc agcaccacat
    27361 cgttctcttt agcctgcata acggcgcaag tcaccgcttc agcacggcct tccatcactt
    27421 ttgcatgtcc ggcatctaac attcccgcca gaatatcgtt gatgatggca cgcggttctt
    27481 cggtacgcgg gttatcgtcc gtcaccaccg ccacgtcagc aaactcttcg gcaattgcgc
    27541 ccatcagtgg acgcttacct ttatcgcgat cgccaccaca gccaaagaca caccacagct
    27601 tgcccgcaca gtgcagacgc gccgcctgta aggctttttc cagtgcatcc ggtgtatgcg
    27661 cgtaatccac caccaccgtc ggtttgcctg gcgcagtgaa cacttccata cgtccgcaaa
    27721 ccggttgcag acgcgcggcg gttttcaaca gatcagccaa tggatagccg agtgccagca
    27781 gtgtcgccag cgcgagcagc aggttgctga cgttaaaagc gcccatcaga tggctttcaa
    27841 tttcgccatc gccccaactt gagctaaagc gaatggtcgc accgctgtcg tgatagttca
    27901 cgtcgatcgc ttttaaccag cgtccgtgac agttcggatt gatatgctct tccattgata
    27961 ccgcaaccgc gtccggcagt tttgccagcc agcggcggcc cacttcatcg tcggcgttaa
    28021 taatcgcctg accgcaatga tgctcagaat aaagcagcca tttcgcggct tcgtagtgtt
    28081 ccatatcacc atgataatca aggtgatcgc ggcttaagtt ggtaaagacc gacgccgcaa
    28141 atttcaatgc cgccacacgg tgctgtacca gcccgtggga ggaaacttcc attgcgcaaa
    28201 acgtcgcgcc ctgatccacc agccccgcca gctcatgctg aacatcgact gccgaaccag
    28261 tggtattttc tgtcgggatc actttcccca gcagaccgtt accaacggtg cccattaccg
    28321 cgctggtttc gccaagcagt tggctccact gcgccagcag ctgggtcgtc gtggttttgc
    28381 cgttggtgcc cgttacgccc acgagacgta aattgtcaga gggttcatgg taaaagcggc
    28441 ccgccagtgc agataaacgc tcgttgagct ggctgagata gatgaccggt acgccgtgca
    28501 tttcacggat ttcaccgtcg gtcgcctcac ctttcgcctc tgcaataatg gcagccacac
    28561 cttgcgctat cgcctgcggg atatatcgac gcccgtccgc ctgatgacct actacagcta
    28621 caaagagatc gcccgccgca gccacacggc tgtcgagtgt catctctcgc agtgctcgcg
    28681 aaggtgcgtc tggcacccac ggagcaagaa ggtcgcgcaa attacgatct gccacctgtc
    28741 ccctcgcctt gattaatcac aaattcattt ttatcgcccg ttgtcagcgc atccggctcg
    28801 atgttcatgg tacgcaatac gccgcccatg atggcaccaa agaccggcgc ggaaacggcg
    28861 ccgccgtagt atttacccgc ctgcggatcg ttgataacaa caaccagcgc gaagcgcggc
    28921 tgactcgcag gcgcaacgcc tgcggtataa gcaatatatt tattgatgta gcgaccgtcc
    28981 ggcccgacct ttttcgcggt accggtttta atggcgatac gatagccttt aatcgccgcc
    29041 ttcacgccgc cgccgcccgg tagcgccacg ctttccatca tatgcaccac ggtgcggaca
    29101 atggattccg ggaagacacg ttcaccggga accggggggt caactttcgt aatcgacagt
    29161 gggcgataaa tgccgtagct gccgatagtt gcgtagactc gcgctaactg taacggtgtt
    29221 accattagcc cgtagccgaa agagaaggtg gccctctcta tgtcagacca ccgttgtttt
    29281 tgaggatata agccactgcg ttctccgacc aaccccaaat tggtcgcttt tcccagtcca
    29341 aaacgtgagt aagtatctac taacgctgag gacggcatcg ctaacgccag cttggaaaca
    29401 ccgacgttac tcgacttctg taataccccg gtcagggtta attcgctgta gcgtgccacg
    29461 tctttgattt cgtggccgtt aattcgataa ggaatggtat tgagtaccga gttttcccgc
    29521 accacgccac gttgcaacgc agtcatcacc accatcggtt taaccgttga gcccggttca
    29581 aacacgtcgg tgatggtacg gttacgcatc gcctctttcg gcgtgccact cagattgtta
    29641 gggttgtatg atgggctgtt agccatcgcc agcacttcac cggtgttgac atccaccagc
    29701 acggcgctac cggactcagc cttgttaaag gccaccgcgt tattcagttc gcgataaacc
    29761 agcgcctgca ggcgttcatc aatactcagc gccaggttgt gcgcagcctg gctgtcagta
    29821 gaagaaatat cttcaattac gcgaccatag cggtctttac gcacaatgcg ctcacccggc
    29881 tgcccggtaa gccatttatc gaaactcttc tcgacgccct caatcccctg gctatcgacg
    29941 ttagtaaagc cgatgaggtg agcagtcact tcgccggatg gataataacg gcgagattct
    30001 tcacgcagat gaatccccgg cagtttcagt tttttgatgt agtccgccat gtcagggttt
    30061 acctgacgcg ccagataaat aaagcgccct ttcgggttgg cgttaatgcg ggctgaaagc
    30121 tgatccagcg gaatattgag cgcgttagcc agcgccttcc agcggtcacc gacgctgata
    30181 ccgccagcgt catgcacttc tttcgggtca gcccaaatcg cttttaccgg cacgctcacc
    30241 gctaacgggc gaccagaacg gtcagtaatc atgccgcggg aggtggaaac ttgctgaacg
    30301 cgaagagaac gcatgtcgcc ctctttcacc agcatatccg gggagataac ttgtaaccac
    30361 gctacgcgtc cgagcagaaa agccagcgcc aggagaatac agccgcataa caacgcaaaa
    30421 cgccaactga taaagttggc atgttcttcc tgacgttttg gtttctgcgt tttcgccgct
    30481 gctttcatgc gtcgcgttta tccttatttt tgcactacga tattttcttg tgacggatca
    30541 acatgctgca tttgcagctt ttccgtggcg atcctttcca cccggctatg gtcgccgagc
    30601 gcattctctt caaggatcag gttgcgccat tcaatgtcta aagcatctcg ctccagcacc
    30661 agttgttcgc gctgagcggt cagtaaacgg gtatggtgcg ccgtggttac cacggtcacc
    30721 gccgtcaaaa taatgcaaat gaacaggcag agtggcagct tcccaaatcg caaaagatcg
    30781 tcaccgataa caccaggcaa tgcatggcgc tcgtggcttc ccatcgatcc tttaactttg
    30841 cttagagctt ctgtcactct gctgatcatg cgttcgtcct ctctgcaata cgcagaactg
    30901 aactacgggc acgaggattc tcagccacct cttcttcgcc cggcattaac ttgcctagtg
    30961 ctcgcagctg acggccaccc agttttttga gctgctcttc agtcatcggt aaccctgccg
    31021 gaacttgcgg accgcggctg ttttcacgca taaaacgttt cacaatacgg tcttccagcg
    31081 agtggaagct gatgatcgaa agccgcccac ccggggccag cacgttgagc gagcttttta
    31141 gcgcctgctc tatctcctcc agttcactgt ttacccaaat gcgcaccgcc tggaaggtac
    31201 gggtcgcggg atgtttaaac ttatctttca ccggcgttgc agcagccacg acttccgcca
    31261 gttctttggt gcgggtcatc ggctgttcgc ggttacgctc gacaatggcg cgggcaatgc
    31321 gtttggcaaa acgctcttca ccataggttt tcaataccca ggcgatatcg gcttcttctg
    31381 cggtttgtag ccattcagcg gctgactgcc cacgggttgg gtccatacgc atgtccagcg
    31441 gaccatcgcg cataaaggaa aagccgcgct cagcatcatc aagttgcggt gaagagacgc
    31501 caagatcgag gagaatgccg tcgatcttgc cgataagatc gcgctcggca acgtattcgc
    31561 ccagcgcgga aaaaggtccg tggatgatgg agaagcgtgg atcatcaata gtcttcgcaa
    31621 cggcgatagc ctgcgggtcg cgatcgatcg ccagcaaacg cccctcttcg ccaagctgcg
    31681 agaggatcag acgtgagtga ccaccgcgac caaaagtccc atcaatgtag atgccatcag
    31741 gacggatatt gaggccatta acggcttcat ccagcagcac cgtagtatgt ttatagtttt
    31801 ccatcatttt atagagacaa gtcctgcagt cgctccgata agtctccggt agccaactgc
    31861 tctgcgtcga tatcttcctt gacctgttga tgccaggttg tttcatccca cagctcaaac
    31921 ttgttgaact gtccaaccag catcacttct tttgtcagcc cggcatgttg ccgcagtact
    31981 ggcgcgatta acaatcgacc tgcgccatcc atctgacatt cgctggcatg acctaacagt
    32041 aggcgctgca cacggcgctc aaccgggttc atgctcgaca gacgcgataa tttttgctcg
    32101 ataatttccc attcaggcag ggggtaaagc agcaggcacg ggtgatgaat gtcaatggtg
    32161 caaaccattt gaccggcagc gttctcaagc agctgttccc gataacgggt aggcactgat
    32221 aagcgccctt tgctgtcgag attgactaac gttgctcccc ggaacatgcc agcctcaccc
    32281 cttattccca ctttgcccca caatttccca ctgaaaggag tttacggagc ggaggaaaag
    32341 cttgtcaagc cagtcacaag gcataccgag gtaaaaaacc cgatgtttac ggctaatatc
    32401 agcgttgttt attttacgtc caacgtgcga agcaaattaa cgttatgaat agctgtaaga
    32461 aaaaaaccct catcgtcatc accgttttaa aatgactcac tattttccac agcaaatata
    32521 aagtgtcagt ttgcgacgcg agcggcattt taggacatat cttccccggt taacagtccc
    32581 tgttgcgtct gtgtagcgct tgatgccagc gggtttgcgc aaaagcaaag aaaagtgctt
    32641 gttaaatcgg caattcatct ctgcatgtaa caaaataatg caaaaacgcg ctgtcgatat
    32701 cacttaatta ttgtgaattt cagctggtga gttaattaac gggcgggttt aaaagcattt
    32761 atcaataata gtcaggaata gtcttattta ctttaaggat attgatgtcc agtcccgtac
    32821 tctacgcgcc agagtgaaat tcacctggcg cgtatttttg ttcgcagctt agctacggct
    32881 gagcacgccg cggcgataga gattacgttt aatgcgcgtt aaaccaggtt ttggcttacg
    32941 cggttcgtcc aggcttgccc ggacaatctc cagcacacgc tctgcgacat cgcggtgacg
    33001 ttgagccact gccagcaccg ggcactgtaa gaagtcgagc agttcattat cgccaaaggt
    33061 ggcaattgcc aggtcagaag gcagtttgcc gtcgcgacgc agcgtgacat ccatcacccc
    33121 ttgcaacaac gcaaacgacg ttgtgaacag cgcctgcggc atcggatgcg tttccagcca
    33181 tttttcgaat aactgggcag ccgcctcccg ctcatagcta ttggcataca gaaaatgcac
    33241 ttcgcgcgga tcatctttcc aggcagtacg gaaaccttgt tcacgcagga agctgacaga
    33301 aagctccggc agcgcgccaa gataaagcac cgtctcggcg ggaaacttac gtaactcttc
    33361 cgccagcatt tcggcatcat cctgatcggc accaaccacg ctggtaaagt gttcacgatc
    33421 gagggcgcgg tcaagcgcga caatcgggaa cgggtcgtta gcccagcgtt gataaaaagg
    33481 atgctcagga ggcaacgacg tcgaaacaat aatggcatcg acctgacgct gtaaaaggtg
    33541 ctcaatacag cgcatttcgt tgtctggctg atcttctgag caggcaatca gcagttgata
    33601 accccgttgc cgcgcctggc gttcaagata gttagcgatg cgggtatagc tggtgttctc
    33661 cagatcgggg atcacaagac caatagaacg tgtgcgtcca gcacgaagcc cagctgccac
    33721 ggcgttcggg tggtaattgt gctcacgcac gacagccatg actttttcaa cggttttgtc
    33781 gctcacacgg tattgcttcg ctttgccgtt aataacatag cttgcagtgg tccgcgacac
    33841 tcccgccagc cgagcgattt catccagttt cacaattgcc ccttgcgtaa aatgtaaaaa
    33901 ccatagccat tgtactggta tgggttaaat tcattaacat ctaagcgcag aaaattaact
    33961 gcggcaactg cttttattcc aggttcccac tgatttcgca aaaaaagccc aacgtgacat
    34021 gttgggctgt aaattgcgca ttgagatcat tctcaacgca ttattttatc gccgcgcgaa
    34081 agtccgacca caccagagcg agcaacttcc acaattttcg ccacatcgcg aatcgatgct
    34141 aaaaatgcat caagcttatc gctggtgcct gctaattgaa cggtataaag cgagggtgtg
    34201 acatcgataa tttgcccacg gaatatttcc gtattacgtt tcacttcgtc acgcccgtaa
    34261 ccgctggcct gaattttcac cagcatgatt tcccgctcaa catgcgcgcc ctgccccaac
    34321 tcactcacgc gcaagacatc gaccagcttg tgtaattgct tttcaatctg ctcaagtact
    34381 ttttcatcgc ccacggtctg gatggtcata cgcgataatg tcggatcgtc ggttggcgca
    34441 acggtcaggc tttcaatgtt gtagccacgc tgggaaaaaa ggccaatcac gcgggataac
    34501 gcgcctgatt cattttcgag taagactgat aatatccggc gcataatcag gttctctccg
    34561 ttttgcttaa ccacatttca tccattccgc ccccgcgaat ctgcatcggg tagacgtgct
    34621 cgctgccatc gacggtaaca tcaacaaaca ccaggcgatt actgcgcacc tgttccagcg
    34681 cctcgctaag tttgctttcc agctcatgcg gatgagaaat ctggatcccg acatgcccat
    34741 aggcttccgc cagacggacg aaatcgggta gcgattgcat ataagattgt gaatgacggc
    34801 cggaatagat catgtcctgc cactgcttca ccatccccag atagcggtta ttgagattca
    34861 ccaccagtac gggcaactcg tattgcaacg cggtagacag ttcctgaatg ttcatctgaa
    34921 tactgccgtc gccagtgacg caaaccacgg tttcttctgg caacgccatt ttgacgccca
    34981 gtgccgcagg taaaccaaaa cccatcgtgc cgaggccacc ggaattgatc cagcgacgcg
    35041 gtttgtcgaa tggataataa agtgcagcaa acatctggtg ctgcccgaca tcggacgtca
    35101 cgtaagcgtc tcccttcgtc aaccgccaaa gagtctcgat caccgcctgc ggtttaatct
    35161 tttcactgtg agtgtcatat ttcaggcact gacgagcgcg ccactgttca atttgctgcc
    35221 accagtcgcg gatctcatcc agcggttgat gggcggattc ttgcgacagg agttcaagca
    35281 tttgttcgag gacctggcga gcatccccca caatcgggat atccgcagtc acggttttag
    35341 aaatggaagt aggatcaata tcgatatgca gaacagtggc atttgggcag tactttgcca
    35401 gattgttcgt cgttcggtca tcaaatcgta ccccgacggc gaaaatcaca tccgcgttat
    35461 gcatcgtcat attggcttcg taggtaccgt gcattcccag catgcccagt gcctgacgat
    35521 gcgttgccgg aaacgccccc agccccatca atgaggaaac aacgggcaga ttcaacgcct
    35581 ctaccgtttc tttcaactgc tgatggcagc ccgccgtgat tgccccaccg cctacgtaga
    35641 caaccggttt ttttgccgct accagcgttt gcagagcacg cttaatttgc cctttatgtc
    35701 cggtagtagt gggattgtaa gaacgcatac tgaccgactc cggccagaca tagggtaatt
    35761 tgttcgccgg attaagaata tctttcggta aatcaacgac cactggtcct gggcgaccac
    35821 ttgccgccag ccagaaagcc tttttcagca cctgcggaat gtcttccgtt tgcttaacca
    35881 gaaaactgtg tttaaccacc ggtcgcgaaa tccccaccat gtcgcactcc tgaaaggcat
    35941 cgtaacctat caacgaggtc gctacctgcc cggaaaggac aactaatgga atggaatcca
    36001 tataagcggt ggcgatgcca gtaatcgcat tggtcgcccc tggacccgac gttaccagca
    36061 cgacgccgac ttccccggtc gcgcgcgcca gaccatcggc catatgcact gccgcctgct
    36121 catgacgaac taaaacatga tcgataccac ccacggtatg caatgcatcg taaatatcaa
    36181 ggactgcgcc tccgggataa ccgaatactt gtttaacgcc ctgatcgata agcgatcgga
    36241 cgaccatctc ggctccagac aacatctcca tggcctgcct cactgtttga cggaaaaaat
    36301 gtgtaaaaca cattaaccgt ccgatcttgc ttaagcaatt ggcagccaga gacataaacg
    36361 ccgggtaata agaataaaca ggaggaaagg tgaaaagaaa cgataaaatc ctccattacg
    36421 aaggataaaa atgataataa atcggctaaa tcccaccact tacagcaatt t
//
LOCUS       DAZUCE010000018        34844 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZUCE010000018 DAZUCE010000000
VERSION     DAZUCE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.018"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..662
                     /locus_tag="ACHY03_000858"
     CDS             <1..662
                     /locus_tag="ACHY03_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFS3904127.1"
                     /translation="DNIKARMFKNELNKEDILKLMAAREKVAGASDKWTKASGLYSAI
                     VKGHTEIVAAWMETAEVIASHYENDKDVVRELLSLSRNNAACSLHIASFKKMSKEVID
                     VYLNAAIHLALQHGFTFDEILEQFTRDFDGKSFSHVITNEDDIHMGLWLKIFKIVVGE
                     NENYLKDVMMQLGAKNNEGKSVISQANGNPVFKELFWKAIDEFNFPQEELNRLNQYRS
                     L"
     gene            complement(801..2165)
                     /gene="mnmE"
                     /locus_tag="ACHY03_000859"
     CDS             complement(801..2165)
                     /gene="mnmE"
                     /locus_tag="ACHY03_000859"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFS3904128.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGISEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2271..3917)
                     /gene="yidC"
                     /locus_tag="ACHY03_000860"
     CDS             complement(2271..3917)
                     /gene="yidC"
                     /locus_tag="ACHY03_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFS3904129.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3920..4177)
                     /gene="yidD"
                     /locus_tag="ACHY03_000861"
     CDS             complement(3920..4177)
                     /gene="yidD"
                     /locus_tag="ACHY03_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFS3904130.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(4141..4500)
                     /gene="rnpA"
                     /locus_tag="ACHY03_000862"
     CDS             complement(4141..4500)
                     /gene="rnpA"
                     /locus_tag="ACHY03_000862"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFS3904131.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(4517..4657)
                     /gene="rpmH"
                     /locus_tag="ACHY03_000863"
     CDS             complement(4517..4657)
                     /gene="rpmH"
                     /locus_tag="ACHY03_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFS3904132.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4887..4967)
                     /locus_tag="ACHY03_000864"
     CDS             complement(4887..4967)
                     /locus_tag="ACHY03_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904133.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            5264..6667
                     /gene="dnaA"
                     /locus_tag="ACHY03_000865"
     CDS             5264..6667
                     /gene="dnaA"
                     /locus_tag="ACHY03_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFS3904134.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6672..7772
                     /gene="dnaN"
                     /locus_tag="ACHY03_000866"
     CDS             6672..7772
                     /gene="dnaN"
                     /locus_tag="ACHY03_000866"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFS3904135.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7772..8845
                     /gene="recF"
                     /locus_tag="ACHY03_000867"
     CDS             7772..8845
                     /gene="recF"
                     /locus_tag="ACHY03_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFS3904136.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8874..11288
                     /gene="gyrB"
                     /locus_tag="ACHY03_000868"
     CDS             8874..11288
                     /gene="gyrB"
                     /locus_tag="ACHY03_000868"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFS3904137.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(11309..11410)
                     /locus_tag="ACHY03_000869"
                     /pseudo
     CDS             complement(11309..11410)
                     /locus_tag="ACHY03_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11528..11926
                     /gene="yidB"
                     /locus_tag="ACHY03_000870"
     CDS             11528..11926
                     /gene="yidB"
                     /locus_tag="ACHY03_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFS3904138.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSELGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            12041..12853
                     /gene="yidA"
                     /locus_tag="ACHY03_000871"
     CDS             12041..12853
                     /gene="yidA"
                     /locus_tag="ACHY03_000871"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFS3904139.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12899..13555)
                     /gene="yidX"
                     /locus_tag="ACHY03_000872"
     CDS             complement(12899..13555)
                     /gene="yidX"
                     /locus_tag="ACHY03_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFS3904140.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13691..13804
                     /locus_tag="ACHY03_000873"
                     /pseudo
     CDS             13691..13804
                     /locus_tag="ACHY03_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13801..14865)
                     /gene="cbrA"
                     /locus_tag="ACHY03_000874"
     CDS             complement(13801..14865)
                     /gene="cbrA"
                     /locus_tag="ACHY03_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFS3904141.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFATLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            14966..16180
                     /gene="yidR"
                     /locus_tag="ACHY03_000875"
     CDS             14966..16180
                     /gene="yidR"
                     /locus_tag="ACHY03_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFS3904142.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSDKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPDGERVSFTYNDHVMHQLDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKATGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWI
                     EGGQLWITETDR"
     gene            complement(16182..16514)
                     /gene="yidQ"
                     /locus_tag="ACHY03_000876"
     CDS             complement(16182..16514)
                     /gene="yidQ"
                     /locus_tag="ACHY03_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFS3904143.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16754..16826
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16820..17233
                     /gene="ibpA"
                     /locus_tag="ACHY03_000877"
     CDS             16820..17233
                     /gene="ibpA"
                     /locus_tag="ACHY03_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFS3904144.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17345..17773
                     /gene="ibpB"
                     /locus_tag="ACHY03_000878"
     CDS             17345..17773
                     /gene="ibpB"
                     /locus_tag="ACHY03_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFS3904145.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            17969..19630
                     /gene="yidE"
                     /locus_tag="ACHY03_000879"
     CDS             17969..19630
                     /gene="yidE"
                     /locus_tag="ACHY03_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFS3904146.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19627..20343)
                     /gene="yidP"
                     /locus_tag="ACHY03_000880"
     CDS             complement(19627..20343)
                     /gene="yidP"
                     /locus_tag="ACHY03_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS3904147.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            20639..22255
                     /locus_tag="ACHY03_000881"
     CDS             20639..22255
                     /locus_tag="ACHY03_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS3904148.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22255..23577
                     /locus_tag="ACHY03_000882"
     CDS             22255..23577
                     /locus_tag="ACHY03_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFS3904149.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23574..24467)
                     /gene="yidL"
                     /locus_tag="ACHY03_000883"
     CDS             complement(23574..24467)
                     /gene="yidL"
                     /locus_tag="ACHY03_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS3904150.1"
                     /translation="MNGKLQTSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24634..26349
                     /gene="yidK"
                     /locus_tag="ACHY03_000884"
     CDS             24634..26349
                     /gene="yidK"
                     /locus_tag="ACHY03_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFS3904151.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26346..27839
                     /gene="yidJ"
                     /locus_tag="ACHY03_000885"
     CDS             26346..27839
                     /gene="yidJ"
                     /locus_tag="ACHY03_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS3904152.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARTEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVNTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDAHFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(27886..28335)
                     /gene="yidI"
                     /locus_tag="ACHY03_000886"
     CDS             complement(27886..28335)
                     /gene="yidI"
                     /locus_tag="ACHY03_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFS3904153.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28444..28791
                     /gene="yidH"
                     /locus_tag="ACHY03_000887"
     CDS             28444..28791
                     /gene="yidH"
                     /locus_tag="ACHY03_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFS3904154.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28781..29143
                     /gene="yidG"
                     /locus_tag="ACHY03_000888"
     CDS             28781..29143
                     /gene="yidG"
                     /locus_tag="ACHY03_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFS3904155.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLSSLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            29140..29637
                     /gene="yidF"
                     /locus_tag="ACHY03_000889"
     CDS             29140..29637
                     /gene="yidF"
                     /locus_tag="ACHY03_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFS3904156.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDTLQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     HPRRDCQACDWHRLCPHAREETPDSMLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29645..30829)
                     /gene="emrD"
                     /locus_tag="ACHY03_000890"
     CDS             complement(29645..30829)
                     /gene="emrD"
                     /locus_tag="ACHY03_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFS3904157.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPIDAPRTRLLTSYKTLFGNGGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(31161..31250)
                     /gene="tisB"
                     /locus_tag="ACHY03_000891"
     CDS             complement(31161..31250)
                     /gene="tisB"
                     /locus_tag="ACHY03_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFS3904158.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31815..31913
                     /gene="ivbL"
                     /locus_tag="ACHY03_000892"
     CDS             31815..31913
                     /gene="ivbL"
                     /locus_tag="ACHY03_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFS3904159.1"
                     /translation="MTTSMFNAKLLPTAPFAAVVVVRVVVVVGNAP"
     gene            32019..33707
                     /gene="ilvB"
                     /locus_tag="ACHY03_000893"
     CDS             32019..33707
                     /gene="ilvB"
                     /locus_tag="ACHY03_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFS3904160.1"
                     /translation="MASSGTTSTRKRFTGAEFIVYFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPVRMRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFASTYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33711..34001
                     /gene="ilvN"
                     /locus_tag="ACHY03_000894"
     CDS             33711..34001
                     /gene="ilvN"
                     /locus_tag="ACHY03_000894"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFS3904161.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34264..>34844
                     /locus_tag="ACHY03_000895"
     CDS             34264..>34844
                     /locus_tag="ACHY03_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904162.1"
                     /translation="MASPINSGTMMSNPCPSTFLDKNRVVAAELNITNNEKKYSPGSN
                     FAKWMLQEIKRLIANIMSGSRSVNTEILNYFHPMPGTENNGNRTWMAATGENEYTVIE
                     QTGGKSFNVFQVGAGKNNIKEILGNTSYSGVAVATIIKSLSEKTAALETHSADTVLRK
                     KLVNSIVMMNTDCSYEMPADIVSNTYDLLNLRI"
BASE COUNT         8438 a         8812 c         9205 g         8389 t
ORIGIN      
        1 tggacaatat aaaagcccgg atgtttaaaa atgaattaaa taaagaagat atattgaaac
       61 tgatggcagc aagagaaaaa gtggcgggag catcagacaa atggacgaag gcatcaggct
      121 tatattctgc gatagtgaaa gggcatacgg agattgttgc tgcctggatg gagacagctg
      181 aagtgatagc cagccattat gaaaatgata aagatgtagt gagagaacta ctgtcgttaa
      241 gcagaaataa tgctgcctgc tctttgcata ttgcaagctt taagaaaatg agtaaagagg
      301 tcattgacgt atatctgaat gcggcgattc acctggcgtt gcaacacggg ttcacttttg
      361 atgaaatttt ggagcagttt acccgtgact ttgatgggaa gtcattctct catgtgataa
      421 ctaacgagga tgatatacat atgggacttt ggctgaaaat attcaaaatt gtggttggtg
      481 aaaatgaaaa ttaccttaaa gatgtgatga tgcaactggg agcgaagaat aatgaaggta
      541 aatctgtaat aagtcaggca aatggcaatc ctgtatttaa agaactattc tggaaagcaa
      601 tagacgaatt taactttccg caagaggagc ttaatcggtt aaaccaatat cgttctctgt
      661 aaagacagga ctgcaagatg ttgaaagtcg ctccagccag tagtaaaggc tgttttggtg
      721 caggatgctc ataaaaaaac aagcgctgtt ccgttctgat agtagaacgg aacagtgccc
      781 tttatatcaa ttaagacgac ttacttacca atacagaagc tggagaaaat ccgccccagc
      841 aggtcgtcag aggtaaattc cccggtgatt tcgcttaagt tctgctgcgc caggcgcaac
      901 tcttccgcca gtagttcgcc tgcccaggca cctaacagtt gcgctttgcc ttgctgcaaa
      961 tgctccgctg cctgttccag cgcctgtagg tggcgacggc gcgccaggaa gccgccttcc
     1021 atgttggtgt caaagcccat gctctgtttg agatggttac gcagcacgtc cacgccttcg
     1081 ccagtccttg cagacagacg aattaacgcg tgaccgttca cttcacttat tcccagcgtt
     1141 tcgccggtga tatcggcttt attgcgcacc acggtgatcg gcagtttcgc tggcagacgg
     1201 gcgataaatt ccggccagat ctctgccgga tcaacggcgt ctgtcgtggt gccatcgacc
     1261 ataaacagca cgcggtcggc ctgttcaatt tcctgccacg cgcgctcgat cccaatacgt
     1321 tcaacttcgt cactggcttc acgtagcccg gcggtatcga tgatatgcag tggcatcccg
     1381 tcaatgtgga tatgctcacg cagcacgtca cgcgtggttc cggcgatatc ggttacgatt
     1441 gccgcttcac ggcccgccag cgcgtttaac aggctcgatt taccggcatt aggacgtccg
     1501 gcaatcacca ccttcatccc ttcgcgcaac aaactaccct gacgtgcttc agcacgcact
     1561 gcatcgagat cggcaatcac gtcattaagc tgggcttcaa tttttccgtc ggagaggaaa
     1621 tcgatctctt catcggggaa atcaattgcc gcttcgacgt aaatgcgcaa gtgggtgagg
     1681 gcttccacca gatggttgac ccgtgcggag aatgcgcctt gcagcgagtt aagtgccgaa
     1741 cgggctgcct gttccgaact ggcatcgata agatcggcaa tcgcctcggc ctgggctaag
     1801 tcgagtttat cgttaagaaa cgcgcgttcg gaaaactcac cagggcgagc aatccgcagg
     1861 ccgggaatgg tcagaatgcg ttttaacagc aggtcgagga tcaccggacc gccatgacct
     1921 tgcagttcca gcacatcttc gccggtgaac gagttcgggc cagggaacca tagcgcaatc
     1981 ccctgatcga gcacgctgcc gtcggcgtct ttaaacggaa gataatcggc gtagcgcggc
     2041 ttaggcagtt tacccagcac ggtttcggca acttcacggg ctttgaagcc ggagatgcgc
     2101 aggatgccaa cgccgccacg tcccggaggc gtggcctggg ctacgatagt gtcattatcg
     2161 ctcatgatgt tcctgttgct ttgtgtggcg gatgcgcggt gcttatccgc cctacgaaaa
     2221 gaaaaaaggc ggtcaactga ccgcccttat tttagcgaaa actcaccgaa tcaggatttt
     2281 ttcttctcgc ggctatgcag gccacgtttt tccagaccac ggtaaatcag ctgctgctga
     2341 ataatggtta ccaggttgct gacgatatag tacagcacca gacctgacgg gaaccacagg
     2401 aagaacacgg tgaagatgac cggcataaag gtcatgatct tctgctgcat cgggtcggtc
     2461 acagtggtcg gcgacatctt ctgaatgaag aacatcgtta cgcccatcag gatcggcagg
     2521 atgtagtacg ggtcctgtgc agacaggtcg tggatccaca gtgcaaacgg tgcctgacgc
     2581 agttcaacgg aacccatcag catgtagtac aacgccagga agattggcat ctggatcagc
     2641 agcgggaagc agccgcccag cgggttaacc ttctcagctt tgtacagcgc catcatttcc
     2701 tggctgatac gctgtttgtc atcgcccaga cgctcacgca ttgcctgaat cttcggctgc
     2761 agcatacgca tcttcgccat ggaggtgtac tgcgctttgg tcagcgggta catgatgcca
     2821 cgaacgataa aggtgatgat gataatggag aagccccagt tacccacaaa gctatggatc
     2881 catttcagca gtttgaacag cggctgagaa atgaaccaca accaaccgta atcaacggtc
     2941 agatccaggt gcggagcaac agctgccatc ttgtcctgga tttccgggcc aacccacagg
     3001 gtgctgttca tcgcgccagt ctgaccaggc tgaaccagta ccggctgaga tttatagccg
     3061 atagcggcga tgccgttacc cagattagcg gtatagaagt tgttggtacc gtcgttatgc
     3121 gggatccacg ccgtcgcgaa atactgttgc agcattgcca cccaaccacc tttcgaagag
     3181 atgttcaggt tttcgttatc ggcaatggta tcgaacttgt atttctcgta cttctcgtca
     3241 ggcgtagagt acgccgcgcc acggaaggtg tgcagtgcga agttgctgct tccggtatcg
     3301 agatgcggtg gcagagtgat ggattgcttc aactgaccaa acgaggagat ttccagcggt
     3361 ttctcgccag cattctgcac gttgtagttg acgttgacag cgtaatcacc acgtttcagg
     3421 acaaacgttt tggtaaacgt gttgcctgcc gcgtcggtat acgtcatcgg tacctgcagt
     3481 tcgttttgac cttcagccag cacataagcg tctttttcaa cgttatacag cggacgcggg
     3541 ccgttagccg ggttatccgg gccatcacga ccggtcagac cgctctgtgc ctgataaata
     3601 aactgcggtg aagtttccag cagctggaac ggctgggtag agttcagctc tttcgggtaa
     3661 gcaggcagca gagcttgctc aacatcacca ccacgggtgt tgatggtcag atcaagcacg
     3721 tcggtcttaa ccgagatcag tttcccctgg ccactggccg gtacgccctg gtcggcggcg
     3781 ctacccgctg cggtggtcgt tgtctgcgtg gtctgttggg cctgaggttg cgggttttta
     3841 tcctgctccc aagcttgcca gatcatgaaa gacacgaaca gcaaagcgat gactaaaaga
     3901 ttgcgttgcg aatccatcgt tagtgttctc tggtatcaaa tggtccgggc gggacgggat
     3961 cgtcaccacc agggtgtaaa gggtggcatt ttaatacgcg tttcaccgtc aaccaactgc
     4021 cttttatcac tccaaacctg cgcaatgcct caattccgta gcttgaacag gttggagtga
     4081 aacgacaatg cggcccgagt agcggactaa tcaggcgttg atagacccga atgagggcta
     4141 tcaggacccg cgagccaggc gacagtggcg gcgccataat ttttccaacg cttccgagag
     4201 agcacggtta tcgaggtcgg caaccccttt tttcgccacc accacgaaat ccatagccgg
     4261 gagttcatgt tggcgcagac ggaagctttc acgcgtcaga cgtttaatcc gattgcgttc
     4321 atgggcgcgt cgaacgtttt tcttggcgac tgtaagaccg atacggggat gccccagcga
     4381 attcaggcgg ccgagaatgg taatttgcgg cgtgccagcc cgttgtggct gctggaagac
     4441 gaatgtgaat tgactgggag ttaacaagcg taactccctg ggaaatgcga gcttaaccac
     4501 tcaggggtta gctttattac ttagaaacgg tcagacgagc gcggccttta gcacgacgac
     4561 gtgccagaac ctgacgacca tttttagtag ccatacgagc acggaagccg tgagaacggt
     4621 tgcgcttcag tacagacggt tgaaaagtgc gtttcatggc gatttctacc taaacttgaa
     4681 taaattcaat ggctttattg gatatccgcc gaaaaatgaa acgatggaca ccgaagccat
     4741 gggtgattaa agaggccgga ttgtaataat tgtacactcc ggagtcaatt ctctttcctt
     4801 atttaccgcg cttttccgca ccttttcgca gggaaaacgt acggcctcac accagtgaaa
     4861 accagcatgg cgcgccgggt ggaggattat acgggctgac gggtaaagcg caaggatcgt
     4921 cctggatctt tattagatcg attaagccaa tttttgtcta tggtcattaa attttccaat
     4981 atgcggcgta aatcgtgccc gcctcgcggc aggatcgttt acacttagcg agttctggat
     5041 catcctgtgg ataaatcggg aaaatctgtg agaaacagaa gatctcttgc gcagtttagg
     5101 ctatgatccg cggtcccgat cgttttgcag gatcttgatc gggcatataa ccgcagacag
     5161 cggttcgtgc gtcaccctca agcagggtct tttcgacgta cgtcaacaat catgaatgtt
     5221 tcagccttag tcattatcga cttttgttcg agtggagtcc gccgtgtcac tttcgctttg
     5281 gcagcagtgt cttgcccgat tgcaggatga gttaccagcc acagaattca gtatgtggat
     5341 acgcccattg caggcggaac tgagcgataa cacgctggcc ctgtacgcgc caaaccgttt
     5401 tgtcctcgat tgggtacggg acaagtacct taacaatatc aatggactgc taaccagttt
     5461 ctgcggagcg gatgccccac agctgcgttt tgaagtcggc accaaaccgg tgacgcaaac
     5521 gccacaagcg gcagtgacga gcaacgtcgc ggcccctgca caggtggcgc aaacgcagcc
     5581 gcaacgtgct gcgccttcta cgcgctcagg ttgggataac gtcccggccc cggcagaacc
     5641 gacctatcgt tctaacgtaa acgtcaaaca cacgtttgat aacttcgttg aaggtaaatc
     5701 taaccaactg gcgcgcgcgg cggctcgcca ggtggcggat aaccctggcg gtgcctataa
     5761 cccgttgttc ctttatggcg gcacgggtct gggtaaaact cacctgctgc atgcggtggg
     5821 taacggcatt atggcgcgca agccgaatgc caaagtggtt tatatgcact ccgagcgctt
     5881 tgttcaggac atggttaaag ccctgcaaaa caacgcgatc gaagagttta aacgctacta
     5941 ccgttccgta gatgcactgc tgatcgacga tattcagttt tttgctaata aagaacgatc
     6001 tcaggaagag tttttccaca ccttcaacgc cctgctggaa ggtaatcaac agatcattct
     6061 cacctcggat cgctatccga aagagatcaa cggcgttgaa gatcgtttga aatcccgctt
     6121 cggctggggg ctgactgtgg cgatcgaacc gccagagtta gaaacccgcg tggcgatcct
     6181 gatgaaaaag gccgacgaaa atgacattcg tttgccaggc gaagtggcgt tctttattgc
     6241 caagcgtcta cgatctaacg tacgtgagct ggaaggcgcg ttgaaccgcg tcattgccaa
     6301 tgccaacttt accggacggg cgatcactat cgacttcgtg cgtgaggcgc tgcgcgactt
     6361 gctggcattg caggaaaaac tggtcaccat cgacaatatt cagaagacgg tggcggagta
     6421 ctacaagatc aaagtcgcgg atctcctttc caagcgtcga tcccgctcgg tggctcgtcc
     6481 gcgtcagatg gcgatggcgc tggcgaaaga gctgactaac cacagtctgc cagagattgg
     6541 cgatgcgttt ggtggtcgtg accacactac ggtgcttcat gcctgccgga agatcgagca
     6601 gctgcgtgaa gagagccacg atatcaaaga agatttttca aatttaatca gaacattgtc
     6661 atcgtaaacc tatgaaattt accgtagaac gtgagcattt attaaaaccg ctacaacagg
     6721 tgagcgggcc gttaggtggt cgtcctacgc taccgattct cggtaatctg ctgttacagg
     6781 ttgctgacgg tacgttgtcg ctgaccggta ctgatctcga gatggaaatg gtggcacgcg
     6841 ttgcgctggt tcagccacac gagccaggag cgacgaccgt tccggcgcgc aaattctttg
     6901 atatctgccg tggtctgcct gaaggcgcgg aaattgccgt gcagctggaa ggtgagcgga
     6961 tgctggtgcg ctccgggcgt agccgttttt cgctgtctac cctgccagcg gcggatttcc
     7021 cgaacctcga tgactggcag agtgaagtcg aatttaccct gccgcaggcg acgatgaagc
     7081 gtctgattga agcgacccag ttttctatgg cgcatcagga cgttcgctat tacttaaacg
     7141 gtatgctgtt tgaaaccgaa ggtgaagaac tgcgcaccgt agcgaccgac ggccaccgtc
     7201 tggcggtctg ttcaatgcca attggtcaat ctttgccaag ccattcggtg atcgtgccgc
     7261 gtaaaggcgt gattgaactg atgcgtatgc tcgacggcgg cgataatccg ctgcgtgtgc
     7321 agattggcag caacaatatt cgcgcccacg ttggcgactt tatcttcacc tctaaactgg
     7381 tggatggtcg cttcccggat taccgccgcg ttctgccgaa gaatccggac aaacatctgg
     7441 aagcaggctg cgatctcctc aagcaggcgt ttgctcgcgc ggcaattctc tctaacgaga
     7501 aattccgcgg tgtgcgcctg tatgtcagcg aaaaccagct gaaaatcacc gccaacaacc
     7561 cggaacagga agaagcggaa gagatcctcg acgttaccta tagcggtgcg gagatggaaa
     7621 tcggcttcaa cgtcagctat gtgctggatg ttctgaacgc gctgaaatgc gaaaacgtcc
     7681 gcatgatgct gaccgattcg gtttccagcg tgcagattga agatgcagcc agccagagcg
     7741 cggcttatgt tgtcatgcca atgagactgt aatgtccctc acccgcttgt tgatccgcga
     7801 tttccgcaac attgaaaccg cggatctcgc tttatctccc ggctttaact ttctggtagg
     7861 tgccaacggc agtggcaaaa ccagcgtgct ggaagccatc tatacgctcg gccatggtcg
     7921 ggcgtttcgc agtttgcaga ttggtcgcgt cattcgccac gagcaggagg cgtttgttct
     7981 ccacgggcga ttacagggcg aagagcgcga gacggcgatt ggcttaacca aagacaaaca
     8041 gggcgacagc aaagtccgca tcgacggtac tgacgggcat aaagtcgcgg aactggcgca
     8101 cctgatgcca atgcagctga taacgccaga agggtttact ttactcaacg gcggccccaa
     8161 atacagaaga gcattcctcg actggggatg ctttcacaac gaacccggat ttttcaccgc
     8221 ctggagcaat ctcaagcgat tgctcaagca gcgcaatgcg gcgctgcgcc aggtgacacg
     8281 ttacgaacag ctacgcccgt gggataaaga actgatcccg ctggcggagc aaatcagcac
     8341 ctggcgcgcg gagtatagcg ccggtatcgc ggccgatatg gctgatacct gtaagcaatt
     8401 tctccctgag ttttctctga ctttctcttt ccagcgcggc tgggagaaag agacagaata
     8461 tgctgaggtg ctggaacgta attttgaacg cgatcgccag ctaacctaca ccgcgcatgg
     8521 cccgcataaa gcggacttac gcattcgcgc cgacggtgcg ccggtggaag ataccttatc
     8581 gcgtgggcag cttaagctgt tgatgtgcgc cttacgtctg gcgcaaggag agttcctcac
     8641 ccgtgaaagc gggcggcggt gtctctacct gatagatgat tttgcctctg agcttgatga
     8701 tgagcgtcgc gggctgcttg ccagccgctt aaaagcgacg caatcgcagg tctttgtcag
     8761 cgcgatcagt gctgaacacg ttatagacat gtcggacgaa aattcgaaga tgtttaccgt
     8821 ggaaaagggt aaaataacgg attaacccaa gtataaatga gcgagaaacg ttgatgtcga
     8881 attcttatga ctcctccagt atcaaagtcc tgaaagggct ggatgcggtg cgtaagcgcc
     8941 cgggtatgta tatcggcgac acggatgacg gcaccggtct gcaccacatg gtattcgagg
     9001 tggtagataa cgctatcgac gaagcgctcg cgggtcactg taaagaaatt atcgtcacca
     9061 ttcacgccga taactctgtc tctgtacagg atgacgggcg cggcattccg accggtattc
     9121 acccggaaga gggcgtatcg gcggcggaag tgatcatgac cgttctgcac gcaggcggta
     9181 aatttgacga taactcctat aaagtgtccg gcggtctgca cggcgttggt gtttcggtag
     9241 taaacgccct gtcgcaaaaa ctggagctgg ttattcagcg cgagggtaaa attcaccgtc
     9301 agatctacga acacggtgta ccgcaggctc cgctggcggt taccggcgag actgaaaaaa
     9361 ccggcaccat ggtgcgtttc tggcccagcc tcgaaacctt caccaatgtg accgagttcg
     9421 aatatgaaat tctggcgaaa cgtctgcgtg agttgtcgtt cctcaactcc ggcgtttcca
     9481 ttcgtctgcg cgacaagcgt gacggcaaag aagaccactt ccactatgaa ggcggcatca
     9541 aggcgttcgt tgaatatctg aacaagaaca aaacgccgat ccacccgaat atcttctact
     9601 tctccaccga aaaagacggt atcggcgtcg aagtggcgtt gcagtggaac gatggcttcc
     9661 aggaaaacat ctactgcttt accaacaaca ttccgcagcg tgacggcggt actcacctgg
     9721 caggcttccg tgcggcgatg acccgtaccc tgaacgccta catggacaaa gaaggctaca
     9781 gcaaaaaagc caaagtcagc gccaccggtg acgatgcgcg tgaaggcctg attgcggtcg
     9841 tttccgtgaa agtgccggac ccgaaattct cctcccagac caaagacaaa ctggtttctt
     9901 ctgaggtgaa atcggcggtt gaacagcaga tgaacgaact gttggcggaa tacctgctgg
     9961 aaaacccaac tgacgcgaaa atcgtggttg gcaaaattat cgatgctgcc cgtgcccgtg
    10021 aagcggcgcg tcgcgctcgt gaaatgaccc gccgtaaagg tgcgctcgac ttagcgggcc
    10081 tgccgggcaa actggcagac tgccaggagc gcgatccggc gctttccgaa ctgtaccttg
    10141 tggaagggga ctccgcgggc ggctctgcga agcaggggcg caaccgcaag aaccaggcga
    10201 ttctgccgct gaagggtaaa atcctcaacg tcgagaaagc gcgcttcgat aagatgcttt
    10261 cttctcagga agtggcgacg cttatcaccg cgcttggttg tggtatcggt cgtgacgagt
    10321 acaacccgga caaactgcgt tatcacagca tcatcatcat gaccgatgcg gacgtcgacg
    10381 gctcgcacat tcgtacgctg ctgttgacct tcttctatcg tcagatgccg gaaattgttg
    10441 aacgcggtca cgtctacatc gctcagccgc cgctgtacaa agtgaagaaa ggcaagcagg
    10501 aacagtacat taaagacgac gaagcgatgg atcagtacca gatctctatc gcgctggacg
    10561 gcgcaacgct gcacaccaac gccagcgcac cggcattggc tggcgaagcg ttagagaaac
    10621 tggtgtctga gtacaacgcg acgcagaaaa tgatcaaccg catggagcgt cgttatccga
    10681 aagcaatgct gaaagagctt atctatcagc cgacgctgac ggaagccgac ctctctgatg
    10741 agcagaccgt tacccggtgg gtgaacgcgc tggtcagcga actgaacgac aaagaacagc
    10801 acggcagcca gtggaagttt gatgtccaca ccaatgccga acaaaacctg ttcgagccga
    10861 ttgttcgcgt gcgtacccac ggtgtggata ctgactatcc gctggatcac gagtttatca
    10921 ccggtggcga atatcgtcgt atctgcacgc tgggtgagaa actgcgtggc ttgctggaag
    10981 aagatgcgtt tatcgaacgt ggcgagcgtc gtcagccggt agccagcttc gagcaggcgc
    11041 tggactggct ggtgaaagag tcccgtcgcg gcctctccat ccagcgttat aaaggtctgg
    11101 gcgagatgaa cccggaacag ctgtgggaaa ccaccatgga cccggaaagc cgtcgtatgc
    11161 tgcgcgttac cgttaaggat gcgattgccg ctgaccagtt gttcaccacg ctgatgggcg
    11221 acgccgttga accgcgccgt gcatttattg aagagaacgc cctgaaagcg gcgaatattg
    11281 atatttaatg gcgttaacaa tgcgagcgtg cctgatgcgc tacgcttatc aggcctacga
    11341 gactcctgca atatattgaa tttgcatgtt tttgtaggcc ggataaggcg ttcacgccgc
    11401 atccggcaag aagctataag aaaagggcgg agatcatctc cgcccttttt atttctgcaa
    11461 tccgcaatta tcatcccgca gttctatact ttttgggtca acacgtaacg ctttacaggt
    11521 gattatcatg gggctttttg atgaagttgt cggcgccttt ctgaaaggcg atgcggggaa
    11581 atatcaggcg attttaagtt gggttgagga gcagggcggc attcaggtgc tgctggaaaa
    11641 actgcaaagt ggcggcttag gggccattct ctcaacctgg ctgagtaatc aacagggcaa
    11701 tcaatcggtg agcggcgagc aactggaatc ggcgctcggc acaaatgcgg tgtccgagct
    11761 cgggcaaaaa cttggcgtgg ataccagtac agcttccagt ttactggcag aacaattgcc
    11821 gaagattatt gatgcgctct caccgcaagg tgaagtgtca ccacaagcca ataacgatct
    11881 gctttccgca ggcatggaac tgctgaaagg gaaactcttc cgctaagcaa atggggagca
    11941 cgcaacgcgc gttgcatccc ccttttccct cgggtgttgt actgattttt gagcggaatc
    12001 gcgttagcat gggtcaggaa ccaatctacc tggggaactc atggctatca aactcattgc
    12061 tatcgatatg gatggcaccc ttctgctgcc cgatcacacc atttcacctg ccgttaaaaa
    12121 tgcgattgct gcagctcgcg cccgtggcgt aaatgtcgtg ctaacgacgg gtcgccctta
    12181 tgcaggtgtt cacaattacc tgaaagagct gcatatggaa cagccgggcg actactgcat
    12241 tacttataac ggcgcgctgg tacagaaggc cgctgatggt agcactgtgg cgcaaaccgc
    12301 tctcagctat gacgactacc gtttcctgga aaaactctct cgcgaagtcg gttctcattt
    12361 ccacgcactg gaccgcacca cgctgtacac cgccaaccgt gatatcagct actacacggt
    12421 gcatgaatcc ttcgttgcta ctattccgct ggtgttctgc gaagcggaga aaatggaccc
    12481 caatacccag ttcctgaaag tgatgatgat tgatgaaccc gccatcctcg accaggctat
    12541 cgcgcgtatt ccgcaggaag tgaaagagaa atataccgtg ctgaaaagtg cgccgtactt
    12601 cctcgaaatc ctcgataaac gcgttaacaa aggtacgggg gtgaaatcac tggccgacgt
    12661 gttaggtatt aaaccggaag aaatcatggc gattggcgat caggaaaacg acattgcaat
    12721 gattgaatat gcaggcgtcg gtgtggcaat ggataatgct attccttcgg tgaaagaagt
    12781 ggcgaacttt gtcaccaaat ccaaccttga agatggcgtg gcgtttgcca ttgaaaagta
    12841 tgtgctgaat taatctatgg gcgggcaaac agcataaacg cgtttgcccg cttactgatt
    12901 attggtttac tgcatataca accagaaatt gcagcggtgc agtaatgatg tccagcgtaa
    12961 cagtcactgg cagtaatgct gcggaaacac catccggaat gcctctgggt gaatagtatt
    13021 cataaaatcg cactttaaat ggatggtaga acgccatcac ctttgagtaa tccatgtttt
    13081 tatttttctc atggattgtg ccttttaggt tttccagtga cctggtacag tgttgaacac
    13141 cacaggcaaa accataatgc gttgccagtg ccttttcttc ttcgttattc cagtagtagt
    13201 aaagcgatat tgtgccggta aatttctttt tccccgcatt taaaacaaat cttgcgtcat
    13261 cggcaacctg aatattgtgc cggttaagtg ctggatcatt gagcatctta acgatgtcat
    13321 cggcaccatc agtgataagg taatccagcg attcccctac aaaaacatag ccttttgttc
    13381 cattactatc ttttgcctgc gatagcccag tgatggtgtc tgactgcgat tgatactttc
    13441 ctgaactacc ctttgcggta tgggaaacca gagcatacga gatacagcct gaaagcatca
    13501 gcgccagtgg gaataaactg gcagccttaa aaaatccctt aaaattcaac ttcatgatta
    13561 atcctttaaa cgagtagcta agccgttgaa agcatactct tctttccctc ctgtttagcg
    13621 cctgatctcc cttttcagat ttctgcccga tataagacct tttcttatga ggtgtttttt
    13681 tgtggactaa tatctgttgc aaggctatgg ctttgccccg gcactggttt atatctccgc
    13741 cgtcgcgttg attggcgcgc tctcttacat cctgctggtg ggcgatgtga agcgcgttgg
    13801 ctaatccttc aactgtggaa tatgtgccac accactgcgc ataatccact ttcttaaagc
    13861 cggtgcggtc aggcatcggc tttttactat cttgccgaag agttttaaac gcagtttgcg
    13921 ggtggcgcgc cagtaagcgg cgttgctctt ctccggctgt ttcaggaaca ccgcacgcag
    13981 aatctctgcg ctatccagcg catagctaat cccctccagc gagctggcgc tgataaatcc
    14041 cgccgcttcg ccaattagaa aggcgttgtc cttaccgcag acaaaatcct gccagcgtga
    14101 gggaaacagc accgtgcatt tttcgctttt caccgcctta ccaaactgga actgaaaggc
    14161 gctcattttc tctttcagcg tcgcgaaacg cgtctgaccg tctttcattg gataggcacc
    14221 gccaaagata aaataaccgt ctttgctgat gctccatgag taacagtcag ttatcgcatt
    14281 atcaaagatg caggagtaga acggcacagg atgtttctct gcgaaccact gctggatagc
    14341 gacatattta cggatttgat gatccgggta gagatgtcgc cgcaccatcg agtttgcgcc
    14401 atcggcaccg accagatagc gggcggtaat atgctgctcc caaccgtctg cacgaaaaat
    14461 gacatgccat ttatcatcct cacgccagat ttttcggcac aggctatcgt gataaacctc
    14521 aacgctggcg gggatcagcg atttcatcca caagtcgaaa gcatggcgat taatattgat
    14581 atagcttcgc tggtagttac gcgtgagcga tgcggcgacg tcgacggttt tgacgctgaa
    14641 aatctgcgga ttagcgatca catcgacagg aagcgtcagc ccatcgcgaa taaaagatcg
    14701 ctgcgcgtcc ggtgccagca gaccgccaca aggtttgctg aaaccttcag taccatgctg
    14761 gtgcttttta tccagcgcga tcacctgcat tttgcctgct aactttcgtg ccaacgccga
    14821 tcctgccggg ccgaggccaa taatcgccac gtcgaaatgt tccatctttt cactcacatt
    14881 catcacgatg gcaaaagact attgtctgaa agtgaagtcc ttgtgaagta tgatggcggg
    14941 aaccgttttg tatcaggacc gcgccatgaa acagataacc tttgctcccc gcaatcacct
    15001 gctcactaat accaatacct ggacgcccga cagccagtgg ctggtatttg acgtgcgtcc
    15061 ttctggggcg tcgtttaccg gcgagaccat tgagcgtgtg aatatccata ccggcgaggt
    15121 cgaggttatc tatcgcgcgt cacagggcgc acacgtcggc gtggtgaccg ttcatccgaa
    15181 gtcagataaa tatgtcttta ttcacggccc tgaaaatcct gatgaaacat ggcattacga
    15241 tttccatcat cgacgcggag tcattgctga aggcggcaag gtgagcaatc tcgatgcaat
    15301 ggatattact gcaccgtaca ccccaggagc gctgcgcggc ggcagccatg tacatgtctt
    15361 tagcccggac ggtgaaaggg tgagctttac ctataacgac catgtaatgc atcaactcga
    15421 ttcggcgctg gatttgcgaa acgtcggtgt tgcggcaccg tttggcccgg tcaacgtaca
    15481 aaagcagcat ccgcgcgaat acagcggtag ccactggtgc gtgctggtga gtaaaaccac
    15541 gcccacgccg cagcctggca gcgatgaaat caatcgtgct tatgaagaag gatgggtagg
    15601 aaatcacgcg ctggcgttta ttggcgacac actttcgcca aagggcgaga aagtgccgga
    15661 gctatttatc gttgagttac cgcaagatga agctggctgg aaagcgacag gtgatgcgcc
    15721 gttaagtgga acggaaacaa ccctgcccgc gccaccgcgt ggcgtcgtgc agcgacgttt
    15781 aacctttacc caccatcggg cttatccggg gttagtcaac gtcccgcgcc actgggtgcg
    15841 ctgtaatccg cagggtacgc aaatcgcgtt tttaatgcgt gatgataacg gcattgtgca
    15901 actgtggctt atctcgccac agggcggcga gccgcgccag ttaacccata acaaaacgga
    15961 tattcagtct gcatttaact ggcatccatc aggagaatgg ttgggctttg tgctggataa
    16021 tcgaattgct tgtgcccatg cgcaaagcgg cgaggttgag tatttaaccg aaaaccacgc
    16081 caatccgcct tctgcggacg ctgtggtctt ctcgccggat ggtcagtggc tggcgtggat
    16141 tgaaggcggc caactgtgga tcaccgaaac tgatcgctaa atcagttatc aggcattctg
    16201 gccggtggaa taaccgcatt ggtggcttgc gcgttggctt cgcttttttc aacgcgtgag
    16261 cgcaccgagc tgtctttgcg gaacacatcc cacggcagta aaagcgtgtc catcacagcg
    16321 gtaaacggca tatcgagaat cgccagcgac ttagtgcccc agttggtttc atcgtcgcca
    16381 atcatggtgg cgctggcgcg ggtgccggga tacgttcctt ctttcccccc ggtatgggac
    16441 atcacgctgg agcatcctcc gagtaatgtc attccgctgc atatcatgaa cgctaacaac
    16501 acatttctta tcataataaa ttcatctgtt gatcgtgggt gttggcctga tgagttatag
    16561 cgatcccttg ctgaaaataa catcatcatt acgtcgctct gtggcggcta tcgcacttta
    16621 acgtttcgtg ctgccccctc agtctatgca atagaccata aactgcaaaa aaaagtccgc
    16681 tgataaggct tgaaaagttc atttccagac ccatttttac atcgtagccg atgaggacgc
    16741 gcctgatggg tgttctggct acctgacctg tccattgtgg aaggtcttac attctcgctg
    16801 atttcaggag ctattgatta tgcgtaactt tgatttatcc ccgctttacc gttctgctat
    16861 tggatttgac cgtttgttta accacttaga aaacaaccag agccagagta atggcggcta
    16921 ccctccgtat aacgttgaac tggtagacga aaaccattac cgcattgcta tcgctgtggc
    16981 tggttttgct gagagcgaac tggaaattac cgcccaggat aatctgctgg tggtgaaagg
    17041 tgctcacgcc gacgaacaaa aagagcgcac ctatctgtac cagggcatcg ctgaacgcaa
    17101 ctttgaacgc aaattccagt tagctgagaa cattcatgtt cgtggggcta acctggtaaa
    17161 tggtttgctg tatatcgatc tcgaacgcgt gattccggaa gcgaaaaaac cgcgccgtat
    17221 cgaaatcaac taattcccta aggccgcctg gcgcggcctg acatctccat gctcgccgtc
    17281 agggagcata tgcgaatctt cggatttgca ggtacttact cgcttcttag aaggagaaat
    17341 gactatgcgt aacttcgatt tatccccact gatgcgtcaa tggatcggtt ttgacaaact
    17401 ggccaacgca ctgcaaaacg ccggtgaaag ccagagcttc ccgccgtaca acattgagaa
    17461 aagcgacgat aaccactacc gcattaccct tgcgctggca ggtttccgtc aggaagattt
    17521 agagattcaa ctggaaggta cgcgcctgag cgtaaaaggc acgccggagc agccaaaaga
    17581 agagaaaaaa tggctgcatc aagggcttat gaatcagcca tttagcctga gctttacgct
    17641 ggctgaaaat atggaagtct ctggcgcaac cttcgtaaac ggtttactgc atattgattt
    17701 aattcgtaat gagcctgaac ccatcgcagc gcagcgtatc gctatcagcg aacgtcccgc
    17761 gttaaatagc taactagcta ttctctttgc cccgccattc tggtggggct ttttttgtct
    17821 ttcttcttca acaatgcgtt aaatttcgac tgtttaagat atttcggcac gttctttccc
    17881 gtgataatgt gcgctataag gcaaatctgc ttcaccgatt cagccataat ccgtagcaat
    17941 taaaatgtta tctcaagaga aatggacgat gagtgatata gcattaacgg tcagtattct
    18001 ggctttggtg gcagtcgtcg gtttgtttat cggcaacgtc aaatttcgcg gcataggatt
    18061 aggtattggc ggcgtgctgt ttggtgggat catcgtcggc cattttgttt ctcaggcggg
    18121 gatgacatta agtagcgata tgctgcatgt tattcaggaa tttggcctga tcctgttcgt
    18181 ttataccatc gggattcagg tagggccggg cttctttgcc tcattgcgcg tctccggatt
    18241 acgcctcaac ctgtttgctg ttctgatcgt catcatcggt ggtctggtta ccgccatcct
    18301 gcataaactg tttgatattc cactgccggt agtgctgggg attttctccg gtgcggttac
    18361 caatacgcca gcgctggggg cagggcagca gattttgcgc gacctgggta caccaatgga
    18421 aatggtcgat cagatgggga tgagttacgc gatggcgtat ccattcggca tttgcgggat
    18481 attgttcacc atgtggatgt tgcgggttat tttccgcgtc aatgtcgaga cagaagccca
    18541 gcagcacgag tcttcacgca ccaatggcgg cgcgctgatc aagactatca atattcgcgt
    18601 tgagaaccct aacctgcatg atttagccat taaagatgta ccgattctca acggcgacaa
    18661 aattatctgc tcgcgtctga aacgcgaaga aaccctaaaa gttccttcgc cagataccat
    18721 tatccaactg ggcgatttgc tgcatctggt gggtcagcca gcggatttac ataatgcgca
    18781 actggtgatt ggtcaggagg tcgatacttc gctgtccacg aaaggcactg atttgcgcgt
    18841 cgagcgtgtg gtggtcacca atgaaaacgt gcttggtaag cgtattcgcg acctgcattt
    18901 taaagaacgc tatgacgttg ttatctcgcg cctgaaccgt gccggggtcg aactggtcgc
    18961 cagtggcgat atcagcctgc agttcggcga tatcctcaat ctggtggggc gtccgtccgc
    19021 aattgatgcc gttgccaatg tgctggggaa tgcgcagcaa aaactgcaac aggttcagat
    19081 gctgccagtg tttattggca tcgggctagg cgtattgtta ggttctattc ccgtctttgt
    19141 gccaggattc ccggccgcgt tgaaactggg gctggcggga ggtccgctga ttatggcgtt
    19201 gatcctcggg cgtatcggca gtatcggcaa gctgtactgg tttatgccgc caagtgccaa
    19261 cctcgcgctg cgggagctgg ggatcgtgct gttcctctcg gtcgttggtc tgaaatctgg
    19321 tggggatttt gtgaataccc tggtcaatgg cgaagggcta agctggattg gttatggtgc
    19381 cctgatcacc gccgttccgc tgattactgt tggcattctg gcgcggatgt tagccaaaat
    19441 gaattacctg accatgtgcg ggatgctggc aggttccatg accgatcctc cggcgctggc
    19501 gtttgctaat aatcttcatc caaccagtgg tgcggcggcg ctctcttacg ccactgtcta
    19561 tccgttagtg atgttcctgc gcattatcac cccccaatta ctggcggtgc tcttctggag
    19621 tatcggttaa ctcttttccg ggtggagtcg ccgtaaatgg tactccacct ggtattcgct
    19681 ggcatttctg aacatcactg aataattcaa aaactccccg ctctcgctat atgacaacga
    19741 ggtgatccgc agcagtggcg tgtgttccgc taccttcatt tgccgcgcca gcagtctatc
    19801 ggcgagcacc ggcgtcagac tttcataatt accgccaatc aaaatcccgc actcttgttc
    19861 aatatattca aacttcgacc cttccagatg ttgcagcgaa agattacgga acagttttac
    19921 cggcatatag ctgtcttcca gcatcagcgg tttcccttcc acaaaacgaa cgcgacggga
    19981 gaagtagatc tgctcgttga tttgaatccg caactggctg gcaatggccg gaggcgcagg
    20041 catgatttca aaaatcagca cctggctggt gaccgttttt ccctgacgtt ttaacacctc
    20101 caccagccca gtcaggctgg cggtttgatg cagcacatct ttgcgcacca ggtaagtgcc
    20161 gctgccgtgg cgtcggacca ccagccccca cgctaccagc aggtcaatgg ctttacggat
    20221 ggtcatccgc gataccgcaa actcttccgc cagctttttt tcaccgggaa gaaggctgtt
    20281 tagcgtgaaa tctgcggaat taagtcgaat tcttaaccgc tccgcaatgc ttttgtagat
    20341 catctctaga tctctttttt gcctgcaagc ccgggtaatg cgcccacaag ttctgcttaa
    20401 tcgattgaaa aaacatctta tttttaaaga tgtgttcgat gctgtgtacc tgtgctcaca
    20461 gaggtctact ttttcgcgaa aacgtagatc tctaccgccc aacgaaaagc atgaaagcga
    20521 tcacgaatcc cattgggtcg tcatgttctg ccgatcgcat cttcctatcc tcgctccagg
    20581 cctgccgcat aaccaatcag gcttcctact tacagaattg agaaaagagg atgtggaaat
    20641 gctcagtcaa attcaacgct ttggcggcgc gatgttcacg ccagtgctgc tgtttccctt
    20701 cgccgggatt gtggtgggtc ttgccatctt gctgcaaaac ccgatgtttg tcggggaatc
    20761 actgaccgat ccgaacagtt tattcgcgca aatcgtacac attattgaag agggcggttg
    20821 gacggtattc cgtaatatgc cgctgatttt tgctgtcggt ttacccattg gccttgctaa
    20881 gcaagcgcag gggcgagctt gtctggcggt gatggtgagt ttcctgacct ggaactattt
    20941 catcaatgcg atgggaatga cctggggaag ttacttcggc gtcgatttca ctcaggacgc
    21001 ggtggcaggt agcggtctga caatgatggc cgggattaaa accctcgata ccagcattat
    21061 cggcgcaatt atcatttccg gcattgtgac ggcgctgcat aaccgtctgt tcgataaaaa
    21121 actgccggtg tttctcggca ttttccaggg gacgtcttat gtggtgatta tcgccttcct
    21181 ggtgatgatc ccctgtgcct ggctgacatt gctcggctgg ccaaaagtac aaatggggat
    21241 tgaatctctg caagcgttcc tgcgttcggc gggtgcgctt ggggtgtggg tttacacctt
    21301 cctcgaacgt attctgatcc caaccggttt acaccacttc atctacggac cgtttatctt
    21361 tggtccggca gctgttgaag gcggaattca gatgtactgg gcgcagcatc tgcaagagtt
    21421 cagtttgagc gccgagccgc tgaaatcgtt gttcccggaa ggcggttttg ccctgcacgg
    21481 taactcaaaa atctttggtg ccgtgggcat ttctttagcg atgtacttca ctgccgcacc
    21541 ggaaaatcgg gtaaaagtgg ctggcttgct gattcccgca accttaaccg ccatgctggt
    21601 gggaattacc gaaccgctgg aatttacctt cctgttcatt tcaccgttgc tgtttgcggt
    21661 acacgctgtg cttgcggcct caatgtcgac cgtaatgtat ctctttggtg tggtgggcaa
    21721 catgggcgga ggtctgattg accaggtttt accgcaaaac tggatcccga tgttcagcaa
    21781 ccacgcggat atgatgctga cccaaatcgc cattgggttg tgctttaccc tgctgtactt
    21841 cgtggttttc cgcaccctga ttctgcaatt caacatgtgc acgccgggac gtgaagatgc
    21901 ggaagtgaaa ctctactcaa aagccgaata caaagcctcg cgaggccaaa ccaccgcggc
    21961 agagccaaaa aaagagctgg atcaggctgc cggtatcctg caagccctgg gcggggtcgg
    22021 caatatctcc agcatcaaca attgcgcgac gcgtctacgt attgcactgc atgacatgtc
    22081 acaaacgctg gatgacgaag tctttaaaaa gctgggagcg cacggcgtct tccgtagtgg
    22141 cgatgccatt caggtgatca ttggtctgca tgtatcccag ctgcgtgaac agctcgatag
    22201 cttaattaat tctcatcaat cagcagaaaa tgttgccatt acggaggcag tataatgacc
    22261 aaattctcag tggttgtcgc tggcggtgga agcaccttta cgccaggcat cgtgttgatg
    22321 ctcctggcga atcaggaccg tttcccgctt cgtgcactga aattttatga taacgatggc
    22381 gcgcggcagg aagtgattgc cgaagcctgt aaagtcatcc ttaaagagaa agcgccggac
    22441 attgcgttta gttacaccac cgatcctgaa gtggcgttca gcgacgttga ttttgtcatg
    22501 gcgcacatcc gcgtcggcaa gtacccgatg cgcgaactgg atgaaaaaat cccgctgcgc
    22561 cacggcgttg ttggtcagga aacttgcgga cccggcggaa tagcgtacgg catgcgttcc
    22621 attggcggcg tcctggaact ggtggattat atggaaaaat attcaccaaa cgcctggatg
    22681 ctcaactact ccaacccggc agccattgtc gcagaagcca cgcgtcgtct gcgcccgaat
    22741 gcgaaaatcc tcaacatctg tgacatgcca atcggtattg aaagccggat ggcgcaaatt
    22801 gttgggctgc aagatcgcaa acagatgcgc gtgcgctact acggcctgaa ccactttggc
    22861 tggtggacat caattgaaga tttgcagggc aacgacctga tgccccagct gcggcaatat
    22921 gtctcgaagc acggttatgt tccaccgcag caagatacgc atactgaagc gagctggaac
    22981 gacacctatg caaaagcgcg ggatgtccag gcactggacc cggatacatt gccgaacacc
    23041 tatctgaaat attatctctt cccggattat gtcgttcagc attccaaccc tgaacatacc
    23101 cgcgcgaatg aggtgatgga acatcgcgag aaacaggttt tcgatgcttg ccgcgccatt
    23161 acggcggcag gaaattcagc ggcgggcaag ctggagattg acgaacatgc gtcatacatc
    23221 gtcgatctgg cggcggcaat tgccttcaac actcaggagc ggatgttgct gattgtgcct
    23281 aacaacgggg caattcataa ctttgatgat gaagcgatgg tcgagatccc gtgtctggtc
    23341 gggcacaacg gaccagaacc actggtggtc ggcgatatcc cgcagtttca gaaagggtta
    23401 atgagtcagc aagtggcggt ggaaaaactg gtcgtggacg cctgggaaca gcgttcatat
    23461 cagcacctgt ggcaggcgat tacgttgtcg aaaacggtac cgagcgcctc ggtcgccaaa
    23521 gctattctgg atgagttgct ggaggccaac aaagcgtact ggccagagtt acgttaattg
    23581 tgtggactgg catccgttgg gtgctggtcc gtattcttta gcacggctga cggcgcataa
    23641 ccaaatttat gtttaaaggc tttgctgaaa tgaaaggagt cgaagaagtt gagcgtatca
    23701 gcgacttcgc caaccgagtt tccttgttgc tttaatagcg atagcgccag atccagacgc
    23761 gcatcgaggt aatattcttt cggcgtcttt ccggtatagc gtaaaaacag acgacgcaac
    23821 caggcttcgc tacaggggat cgtggcagcc atatcggcta cgctccagcg ttgttgcaga
    23881 ctggcatgta aagtggcaat taatttttca atttgccgcc gttgtggatc ttttttaccg
    23941 tctgcgtaca taagacaaat ccactgataa ataattttcg tcagaaaagc gactgccaga
    24001 ttatttttta ttgcttctgg tgaagttatt aactcagcaa cttcggtgag ttcctgatta
    24061 taaatttcgc cattgtaaat aacgctttgc tgaccgacgg gaatatccat catactggtg
    24121 ggggtaaatt ccatccagta ttgttcccag actaaacctt cacagtgata agagtgaata
    24181 tccattggct ttaaaaatat aatacagttg ccatgcagtg ttatttgttc gccattcttt
    24241 aaaagaattt tcccacatcc ttgtacggta tacaccgcaa cccatgagtt ggcgataagc
    24301 ggtttcttct tgtcccgcgg ccagatcacc cggtaatgct cgtcggcgta ggtatgccag
    24361 agcgagatca gagaaattcc gctggagatc acattttcgt taatcaataa gggaatattg
    24421 tatggagttt cgttttttac atctgaagtt tgcaattttc cattcatttt aatatccctc
    24481 cggcgaatta tgctcacaac ctgaacttaa cagaaaacaa tccgttcagg gatacgacgt
    24541 ttgtatttta agaatctgac tgcctgaccc gacgcatttt acctctccct atattcatgc
    24601 gtccgggaca taaacataaa taagggctat gagatgaatt cgttacaaat cttgagtttt
    24661 gtcggtttta cgctgctggt ggcgatcatc acctggtgga aggttcgcaa aacagatacc
    24721 ggatcgcaac aaggctattt tcttgccgga cgttcactaa aagcgccggt tattgccgct
    24781 tcgttaatgt taaccaacct ttccacggaa caactggttg gtctttccgg gcaggcctac
    24841 aaaagcggca tgtcggtgat gggctgggaa gtgacttcag cggtgacgct gatcttcctc
    24901 gcgctaatct ttttaccgcg ctatctgaag cgcggcattg ccaccatccc cgattttctg
    24961 gaggaacgtt atgataaaac gacgcgtatt atcatcgact tctgcttcct cattgccacc
    25021 ggcgtctgct ttctgccgat tgttctctac tccggcgcgt tggcgctcaa cagcctgttt
    25081 cacgtcgggg aatcgctaca gatttctcac ggtgcggcta tctggctatt ggtaattttg
    25141 cttggtctgg cgggaatttt gtatgcggtg atcggcggac tgcgcgcaat ggcagtggcg
    25201 gactccatca acggtattgg gctggttatc ggcgggttga tggtgccggt atttggcctg
    25261 atcgcgatgg gcaagggcag ctttatgcag ggcattgagc aaattaccac cgttcacgcc
    25321 gagaaattaa actcagtcgg tggcccgacc gatcccttgc cgattggcgc ggcatttacc
    25381 ggtttaattc tggtgaacac cttttactgg tgtacaaatc agggcatcgt gcaacgcacg
    25441 ctggcgtcaa aaagcctggc ggaagggcaa aagggggcgc tgttaacggc ggtgctgaaa
    25501 atgctcgacc cgctggtact ggtgctgcca gggttgattg cgtttcatct gtatcaggat
    25561 ctaccgaaag ccgacatggc ctacccgacg ctggtcaata acgttctgcc agtgccactg
    25621 gtgggtttct tcggcgcggt gttatttggt gcggtgatca gtaccttcaa cggctttctg
    25681 aatagcgcca gtacgttatt cagtatgggt atttaccgtc gcatcattaa ccagaatgcc
    25741 gagccgcagc agctggtcac cgtcgggcgc aaatttggtt tctttatcgc tatcgtttcg
    25801 gtgctggtcg cgccgtggat cgccaacgcg ccgcaggggc tgtatagctg gatgaaacag
    25861 ctcaacggca tttacaacgt gccgctggtt accatcatca ttatgggctt tttcttcccg
    25921 cgcatcccgg cgctggcggc aaaagtggcg atggggattg gcataatcag ctacatcacc
    25981 atcaactatc tggtgaagtt cgacttccat ttcctctatg tgctggcctg tacgttctgc
    26041 atcaacgtgg tcgtgatgct ggtgatcggt tttatcaaac cgcgcgccac gccgttcacc
    26101 ttcaaagatg cgtttgcggt ggacatgaaa ccgtggaaaa acgtcaagat cgcgtcaatt
    26161 ggcatcctgt tcgcgatgat tggcgtctat gccgggctgg ctgaattcgg cggctacggt
    26221 acgcgctggt tagcgatgat cagttatttc attgccgccg tagtgattgt ctacctgatt
    26281 tttgacagct ggcggcatcg tcacgaccca gccgtaacct ttactcccga cgcgaaggat
    26341 agcctatgaa acgccccaat tttctgttca tcatgaccga tacccaggcc accaatatgg
    26401 tcggttgcta tagcggtaaa ccgctgaata cgcaaaatat tgatagtctg gcggcggaag
    26461 gtattcgctt taattccgcc tacacctgtt caccggtttg tacaccggcg cgcgccgggc
    26521 tgtttaccgg tatctacgct aaccagtccg ggccgtggac caacaacgtc gcgccgggta
    26581 aaaacatctc cactatgggg cgctacttta aggatgccgg ctatcacacc tgttacatcg
    26641 gcaaatggca tctcgacggt catgactatt tcggcactgg cgagtgtccg ccggagtggg
    26701 acgctgatta ctggttcgat ggggcgaact atcttagcga actgacggaa aaagagatta
    26761 gcctgtggcg caatggccta aacagcgtcg aagatttaca ggcgaaccat attgacgaaa
    26821 ccttcacctg ggcgcaccgc atcagcaatc gggcggtgga ttttctgcaa cagcccgcgc
    26881 gcaccgagga acccttcctg atggtggttt cgtatgatga gccgcatcac ccgttcacct
    26941 gtccggtgga gtatttagag aaatacgctg atttttacta cgagctgggc gagaaagcac
    27001 aggatgacct ggcgaacaaa ccagaacatc accgcttatg ggcgcaggcg atgccatcgc
    27061 cagtcggtga tgacgggctt tatcaccatc cgctctattt tgcctgtaat gactttgttg
    27121 atgaccaaat aggacgggtc atcaatgcct taacgccaga gcaacgtgaa aatacgtggg
    27181 ttatttatac atccgatcac ggcgaaatga tgggcgcaca taagctgatc agtaaaggag
    27241 cggcgatgta tgacgacatc acccgtattc cgctgatcat ccgttcgccg caaggggagc
    27301 ggcgacaggt caatacgcca gtcagtcata tcgatttact gccgacaatg atggcgctgg
    27361 cagatattga aaaaccagag attctgccgg gggaaaatat ccttgccgtg aaagagccac
    27421 gcggcgtgat ggtggaattt aaccgctacg agattgagca tgacagcttt ggcggtttta
    27481 ttccggtgcg ttgctgggtg acggatgact ttaaactggt gctcaacctc ttcaccagtg
    27541 atgaacttta cgatcgccgt aatgatccaa atgaaatgca taacctgatc gatgatgccc
    27601 attttgccga cgttcgcagc aaaatgcatg acgccttatt ggattatatg gacaaaattc
    27661 gcgatccgtt ccgcagttac caatggagcc tgcgtccgtg gcgtaaagat gcacagccgc
    27721 gctggatggg ggcatttcgt ccacgcccac aagatggtta ttcgccagtg gtacgcgact
    27781 atgacaccgg cctgccgaca caaggggtga aggtggaaga gaaaaaacag aagttctgac
    27841 gataatgccc gctgatgaac acccggcggg cattattcag gctgcttaaa ccagtgaacc
    27901 gccagtcagg aaggaaaaga taaggaatat cagcaaaatg ctgcacacga tgccgatacc
    27961 aaaaagcaag gtatggaaag ccagcgtccc gccgccaaaa accagcgcgc cgcgaatgcc
    28021 ccagcgattt tccttacgca taccgccaat aaatgcgcaa agtagcgcag caatggccag
    28081 tgcgataccg ctttgatcga ggattttatc gacatccacg tcctcttttc tctccacggt
    28141 ggtaattttt tcgcctttta ttccggcgag aagacctctt ttaatttcag ccgttttgtc
    28201 cgccacgatg ctttcaagcg ttggcggtgg tgcggaaaaa gggccgaatg aaaagtggat
    28261 aatccccatc attaaagcga tggcaccgaa tagcatgccc aatgaactga ttttattttg
    28321 cgctatgata cccatctgac ctccttgtag cggatggcaa aagcatatca ggaaacgggc
    28381 ggacgaaatt cttaaaaaaa accgctaaag gtagagcgtt agtgaaattg caaaggagca
    28441 acaatgaaaa tttcccgcct tggagaagcg cccgattacc gcttctcgct ggctaatgag
    28501 cgtacttttc tggcgtggat ccgtaccgca ctaggatttc tggcggcggg ggttgggctt
    28561 gatcaacttg cgccagattt cgccacgccc gtcattcgcg aactgctggc gttgttgttg
    28621 tgtctgtttt ccggcgggtt agcgatgtat ggctacttgc gctggttacg caatgaaaaa
    28681 gcgatgcgtc tgaaagagga cctgccctac accaacagct tattaatcat cagcttaata
    28741 ttgatggtcg tagcagtgat cgttatggga ctggtgttgt atgccggata gccgcaaagc
    28801 cagacgcatt gccgacccag ggctgcagcc ggagcgtaca tcactggcgt ggtttcgtac
    28861 catgctgggc tacggcgcgt tgatggcgtt ggctatcaaa cacaactggc accaggcggg
    28921 catgttattc tggatttcga ttggcatcct cgccatcgtg gcgctgatcc tctggcacta
    28981 cacccgtaat cgcaatttaa tggatgtcac gaatagcgat ttttcccaat ttcacgtagt
    29041 ccgtgacaaa tttttgagct ccctcgcggt gttatctctc gcaatactgt ttgctgtaac
    29101 gcatatacat caacttatcg tatttattga gagagtcgca tgacaggaag tcaggtcatt
    29161 gatgctgaag aggacaggca taaactggtt gtagagtata aggatactct gcaacctgcc
    29221 gatttttatc acaatttcaa acagcggggc atccgctccg tacagcttat tccccatctt
    29281 gaatttgatg accggggcga tatgacggcc gcttcggtga cagcagagct gtgggggaaa
    29341 tttttaattg ccctgtttga gtgttgggtg cgggcagata tcagtcgtat ttcgattgag
    29401 ctttttgatg ccactttaca aaaatggtgt ggcagcgaaa acccgcaccc gcgccgtgac
    29461 tgccaggcgt gcgactggca tcggctgtgt ccgcacgctc gggaggagac gccagacagc
    29521 atgctatgtg cgggctatca ggcgttttat tcttactccg caccgcatat gcgggtaatg
    29581 cgtgatctga taaagcagca tcgttctccc atggagctga tgacgatgct gcggtgatgt
    29641 gcgcttaaac gggctgcccc tgatgcgaca tccgcgtcgc cagcggtagc cagcacagca
    29701 cgatcaacaa tcccattaag gtcatcaaca accccaggct gccctgaccg gtttgcggca
    29761 acatcgctga tagcgacgcc agcacgccgg aaccaatgtt ttgcagacca ccgaccagcg
    29821 cgccagccgt gcccgccagg aaggggaacg gctccatcgc gccgctggtc gccagcggaa
    29881 acagcatccc ggcaccgaaa aagaacagcg cggcgggaac aagcagcgtc cagacattca
    29941 tcacgccaaa ccagtcgggg atccacatca gcaagccagc cagcaggcag cagataaccg
    30001 actgccacat caacgttgag aagcgtttat taggacgccc ggcaaaccat gccccaaaaa
    30061 atgccgccgg aatcggcaga ataaacaaaa tactgaccgt catactgctc agccctaaca
    30121 ccgcgcccat cagcacgccg gagcaggctt caaaggcggc aatcccggcc agaccgccaa
    30181 tcagcatcag caaataacag ttaaaaccgc cgttaccgaa aagcgtttta taactggtaa
    30241 gcaggcgcgt gcgcggtgca tcgatcggac gcgtttccgg catccagcgg gccatactga
    30301 aggtcacacc agcacaaaga accaacaaaa agagataaca ggcgcgccag ttccacatcg
    30361 tatccagcag accgccgatt agcggtgcga gcaacggact gacaagaatc cccatgttta
    30421 acaggctgtt agcatggcgc aactgtgtcc gttcatataa atcacgcggc aaagtacgcg
    30481 ccattacgcc gccaacaccg gtgcccatcc cctgcatcgc gctggcggca atcaataccg
    30541 tcaaactgga ggtcgtgacc gcgaccagcg ttgccagcat aaaaatggac attccgacga
    30601 ggatcactgg tcggcgaccc acgcggtcgg atatcgggcc ataaaacagc tgcgagacac
    30661 cgtaagtcag cagataagcg cccattacgc tctgcaccgc cccttcacgg acgttgagat
    30721 cgcgcgccat atcggcaata gctggaatat aaatggtttg cgccatctga ccgacggcca
    30781 cgagtaatac caacatcaat aacaaattga cgtttctatg ccttttcatt atcacggatg
    30841 cttttataaa aataattaga ataagtcact acgcattccc ataaatgcgg gagaggaatc
    30901 tatcacattg atgaaggtta gccagatatg tgccgggaat ggcgaaaagc aaaacggcag
    30961 aatatgtaaa caaaagcggc aataaatgtt gccggaaggg attacgtgcg aactgtaacc
    31021 ctctccctga aagggtgagg ggcacaggca gcgcaggtgc aaacattctg ctggtagaag
    31081 aagcccccaa ctccaacccg cacgctaaat ataaaagggg agcggttacc cgctcccctt
    31141 tggtgcgact tgaatctgaa ttacttcagg tatttcagaa cagcatcaag tagttgcagt
    31201 gctgcaacaa tgagtttgag gataagaatg gcgatatcca ccaggctcat acgcgtctcc
    31261 tgtggttcag gagcactgcc agctgacgta cctttccgct gcctgttgcc agctgttgtg
    31321 cacgcggtat ctcaaggaga aacgttgtgc ttgttgacat aacacagtgt gctcacgcgg
    31381 ggttaaggcg ctgacggcac cacccgtttc agccaggact tagtgcgccg ggtaacgatg
    31441 cggcccccgc ataacacatt gcgtacagtg atattataga actttactgt ataaataaac
    31501 agtaatattt ggacaaaacg caaactgtga tcgatactca actatgcact aaatacgtca
    31561 aaattcgtgc cgaaattgcg cgttctgcgc ggaacacgta tactttcagt gttgacataa
    31621 tacagtgtgc tttgcggtta ccagccgcag gcggctgacg aaacctcgct ccggcggggt
    31681 tttttgttac ctgcaattca gtgcaaaacg tgatcaaccc ctcaattttc ccttgctgaa
    31741 aaattttcca ttgtctcccc tgtaaagctg tgcttgtata aatattgtta aacacaaaac
    31801 caacaaggtc cccaatgact acttccatgt tcaacgcaaa actactacca actgcgccat
    31861 tcgccgcagt ggtcgtcgtg cgtgtggtgg tggtcgtcgg caatgcgccg tagggactgg
    31921 aacaacacac gattccaaaa ccccgccggc gcaaaccggg cggggttttt cgtttaagca
    31981 cctcccggaa agtcggccca gaagaaaagg actggagcat ggcaagttcg ggcacaacat
    32041 cgacgcgtaa gcgctttacc ggcgcagaat ttatcgttta tttcctggaa cagcagggca
    32101 ttaagattgt gacgggtatt ccgggcggtt ctatcctgcc tgtttacgat gccttaagcc
    32161 aaagcacgca aatccgccat attctggctc gccatgaaca gggcgcgggg tttatcgctc
    32221 agggaatggc gcgcaccgac ggtaaaccgg cggtctgtat ggcctgtagc gggccgggtg
    32281 cgactaacct ggtgaccgcc attgccgatg cgcggctgga ctccatcccg ctgatttgca
    32341 tcactggtca ggttcccgcc tcgatgatcg gcaccgacgc cttccaggaa gtcgatacct
    32401 acggcatctc tatccccatc accaaacaca actatctggt cagacatatc gaagaactcc
    32461 cgcaggtcat gagcgatgcc ttccgcattg cgcaatcagg ccgccctggc ccggtgtgga
    32521 tagacattcc taaggatgta caaacggcag tttttgagat tgaagctcag cccgcggtgg
    32581 cagaaaaagc cgctgcaccc gcctttagcg aagaaagcat tcgtgacgcg gctgcgatga
    32641 ttaacgccgc caaacgcccg gtgctttatc tgggcggcgg tgtgatcaat gcgcctgtgc
    32701 ggatgcgtga actggcggag aaagcgcaac tgccaactac catgacctta atggcgctgg
    32761 gtatgctgcc aaaagcgcat ccgctgtcgc tgggtatgct ggggatgcac ggcgtgcgca
    32821 gcaccaacta tattttgcag gaggcggatt tgttgattgt gctcggtgcg cgttttgatg
    32881 accgggcgat tggcaaaacc gagcagttct gcccgaatgc caaaattatt catgtcgata
    32941 tcgaccgcgc agagctgggt aaaatcaagc aaccgcatgt ggcgattcag gcggatgttg
    33001 atgacgtgct ggcgcagttg atcccgctgg tggaagcgca accgcgtgca gagtggcacc
    33061 agttggtggc ggatttgcag cgcgaatttc cgtgtccaat cccgaaagcg tgcgatccat
    33121 taagccatta cggcctgatc aacgccgttg ccgcctgtgt cgatgacaat gcgattatca
    33181 ctaccgacgt tggtcagcat cagatgtgga ccgcgcaagc ctatccgctc aatcgcccac
    33241 gccagtggct gacctctggc gggctgggca cgatgggctt tggcctgcct gcggcgattg
    33301 gcgcggcgtt ggcgaacccg gatcgcaaag tgttgtgttt ctccggcgac ggcagcctga
    33361 tgatgaatat tcaggagatg gcgaccgcca gtgaaaacca gctggatgtc aaaatcattc
    33421 tgatgaacaa cgaagcgctg gggctggtgc atcagcaaca gagtctgttc tacaagcagg
    33481 gcgtttttgc ctccacctat ccgggcaaaa tcaactttat gcagattgcc gccggattcg
    33541 gcctcgaaac ctgtgatttg aataacgaag ccgatccgca ggctgcattg caagaaatca
    33601 tcaatcgccc tggtccggcg ctgatccatg tgcgtattga tgccgaagaa aaagtgtacc
    33661 cgatggtgcc gccaggtgcg gcgaatactg aaatggtggg ggaataagcc atgcaaaaca
    33721 caactcatga caacgtgatt ctggaactca ccgttcgcaa ccatccgggc gtaatgaccc
    33781 acgtttgtgg cctttttgcc cgtcgcgctt ttaacgttga gggcattctt tgcttgccga
    33841 ttcaggacag cgacaaaagc cacatttggc tactggtcaa tgacgaccag cgtctggagc
    33901 agatgataag ccagatagac aagctggaag atgtggtgaa agtgcagcgt aatcagtccg
    33961 atccgacgat gtttaacaag attgcggtgt tttttcagta aggagcgaca ttaaaccgct
    34021 cgggggggta ctgatgagcg atttttgata gatccgttca gatatatagg gtgaaattgt
    34081 gccagagacc atctcaggca caatttatta gccctgaatg ttaatttaag tcgctgcacc
    34141 agtaatttgg gggttcacag ttagggctaa aatttccagc gatggtgtta aattctgatt
    34201 gctgtctata ccaatggtca tgaccagcat tctattaatt aatggttcat cgtgaggtat
    34261 attatggcaa gccctataaa cagcggaaca atgatgtcaa atccctgccc ttccaccttt
    34321 ttggataaaa atagagttgt tgctgctgag cttaatatca caaataacga gaaaaaatat
    34381 tcacctggca gtaatttcgc aaaatggatg ttgcaggaaa taaaacgctt aatagcaaat
    34441 attatgtccg gctctcgcag tgttaatacc gaaatcctta actattttca ccctatgcca
    34501 ggcactgaaa ataatggcaa tcgtacctgg atggcggcaa ctggagagaa cgaatacacc
    34561 gtaattgaac aaacaggtgg caaatccttt aatgtttttc aggttggtgc aggtaaaaat
    34621 aacataaaag aaatattggg gaatacatca tattctggag tggctgtagc gactattatc
    34681 aaatcgctgt ctgaaaaaac tgctgcgtta gaaacacatt ctgctgacac ggttttgagg
    34741 aaaaaattag ttaatagtat tgttatgatg aatacggatt gtagttacga aatgcctgca
    34801 gacattgtga gtaatacata tgatttatta aacctgcgaa ttca
//
LOCUS       DAZUCE010000019        34602 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZUCE010000019 DAZUCE010000000
VERSION     DAZUCE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.019"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            245..2950
                     /gene="malT"
                     /locus_tag="ACHY03_000896"
     CDS             245..2950
                     /gene="malT"
                     /locus_tag="ACHY03_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFS3904163.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2993..4009)
                     /gene="rtcA"
                     /locus_tag="ACHY03_000897"
     CDS             complement(2993..4009)
                     /gene="rtcA"
                     /locus_tag="ACHY03_000897"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFS3904164.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCMLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVTSFNTLQLGERGNIVRMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(4013..5239)
                     /gene="rtcB"
                     /locus_tag="ACHY03_000898"
     CDS             complement(4013..5239)
                     /gene="rtcB"
                     /locus_tag="ACHY03_000898"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFS3904165.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWVQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5426..7024
                     /gene="rtcR"
                     /locus_tag="ACHY03_000899"
     CDS             5426..7024
                     /gene="rtcR"
                     /locus_tag="ACHY03_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFS3904166.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSNFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLEVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEALQDQHSSS"
     gene            complement(7006..7764)
                     /locus_tag="ACHY03_000900"
     CDS             complement(7006..7764)
                     /locus_tag="ACHY03_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFS3904167.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7781..8611)
                     /gene="glpG"
                     /locus_tag="ACHY03_000901"
     CDS             complement(7781..8611)
                     /gene="glpG"
                     /locus_tag="ACHY03_000901"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFS3904168.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8656..8982)
                     /gene="glpE"
                     /locus_tag="ACHY03_000902"
     CDS             complement(8656..8982)
                     /gene="glpE"
                     /locus_tag="ACHY03_000902"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFS3904169.1"
                     /translation="MDQFECINVADAHQKLQEKEVVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9172..10677
                     /gene="glpD"
                     /locus_tag="ACHY03_000903"
     CDS             9172..10677
                     /gene="glpD"
                     /locus_tag="ACHY03_000903"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFS3904170.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLNVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLSAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10867..10968)
                     /locus_tag="ACHY03_000904"
                     /pseudo
     CDS             complement(10867..10968)
                     /locus_tag="ACHY03_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10968..11960)
                     /locus_tag="ACHY03_000905"
     CDS             complement(10968..11960)
                     /locus_tag="ACHY03_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS3904171.1"
                     /translation="MNKFTGVLLLGTALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPKPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAKEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFLSPQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(11986..13494)
                     /locus_tag="ACHY03_000906"
     CDS             complement(11986..13494)
                     /locus_tag="ACHY03_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS3904172.1"
                     /translation="MMPNRKWILTSLIMTFFGIPILAQFLAAVVAMLGVGLAGIIEVC
                     NILITPTIYLLLNVFMLTLGAIIIFFSGRVWAGDSAPENREIAVWRQCFFLLPALLTL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWMVIIPVGAHISFSL
                     GYAWPTRYPLSGTSGLRCRNLLLFLLLLLGIVAGYQAHLYKQQNPGVGVRENIDIWAW
                     RPDKLNNRLTPLRGKPQIQFRQNWPRIDGATAAYPIYASAFYALSVIPEDFHVWDYLE
                     NSRTPEAYNKIVKGDADIIFVAQPSGGQKKRAKESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWHTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASVMKGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGITSTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYPTME"
     gene            complement(13620..16067)
                     /gene="glgP"
                     /locus_tag="ACHY03_000907"
     CDS             complement(13620..16067)
                     /gene="glgP"
                     /locus_tag="ACHY03_000907"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFS3904173.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(16086..17519)
                     /gene="glgA"
                     /locus_tag="ACHY03_000908"
     CDS             complement(16086..17519)
                     /gene="glgA"
                     /locus_tag="ACHY03_000908"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFS3904174.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(17519..18814)
                     /gene="glgC"
                     /locus_tag="ACHY03_000909"
     CDS             complement(17519..18814)
                     /gene="glgC"
                     /locus_tag="ACHY03_000909"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFS3904175.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(18832..20805)
                     /gene="glgX"
                     /locus_tag="ACHY03_000910"
     CDS             complement(18832..20805)
                     /gene="glgX"
                     /locus_tag="ACHY03_000910"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFS3904176.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(20802..22988)
                     /gene="glgB"
                     /locus_tag="ACHY03_000911"
     CDS             complement(20802..22988)
                     /gene="glgB"
                     /locus_tag="ACHY03_000911"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFS3904177.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(23261..24364)
                     /gene="asd"
                     /locus_tag="ACHY03_000912"
     CDS             complement(23261..24364)
                     /gene="asd"
                     /locus_tag="ACHY03_000912"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS3904178.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            24556..25149
                     /gene="yhgN"
                     /locus_tag="ACHY03_000913"
     CDS             24556..25149
                     /gene="yhgN"
                     /locus_tag="ACHY03_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFS3904179.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(25195..26490)
                     /locus_tag="ACHY03_000914"
     CDS             complement(25195..26490)
                     /locus_tag="ACHY03_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS3904180.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDKQSANSQLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(26397..28493)
                     /locus_tag="ACHY03_000915"
     CDS             complement(26397..28493)
                     /locus_tag="ACHY03_000915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFS3904181.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(28633..29973)
                     /gene="gntU"
                     /locus_tag="ACHY03_000916"
     CDS             complement(28633..29973)
                     /gene="gntU"
                     /locus_tag="ACHY03_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFS3904182.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(29977..30504)
                     /gene="gntK"
                     /locus_tag="ACHY03_000917"
     CDS             complement(29977..30504)
                     /gene="gntK"
                     /locus_tag="ACHY03_000917"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFS3904183.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(30643..31638)
                     /gene="gntR"
                     /locus_tag="ACHY03_000918"
     CDS             complement(30643..31638)
                     /gene="gntR"
                     /locus_tag="ACHY03_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFS3904184.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTAAIIARGHRHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(31862..32557)
                     /gene="yhhW"
                     /locus_tag="ACHY03_000919"
     CDS             complement(31862..32557)
                     /gene="yhhW"
                     /locus_tag="ACHY03_000919"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFS3904185.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(32680..33717)
                     /gene="yhhX"
                     /locus_tag="ACHY03_000920"
     CDS             complement(32680..33717)
                     /gene="yhhX"
                     /locus_tag="ACHY03_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS3904186.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVV
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGCVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            34051..34539
                     /gene="yhhY"
                     /locus_tag="ACHY03_000921"
     CDS             34051..34539
                     /gene="yhhY"
                     /locus_tag="ACHY03_000921"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFS3904187.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         8512 a         9388 c         8557 g         8145 t
ORIGIN      
        1 tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca gtattcacgc
       61 tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga cacagtgcaa
      121 attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc cgaccttata
      181 accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag tgaagtgatt
      241 aactatgctg attccgtcaa aattaagtcg tccggttcga ctcgaccata ccgtggttcg
      301 tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc tgatcacaag
      361 tcctgcgggc tacggtaaga ccacgctcat ttcccagtgg gcggcaggca aaaacgatat
      421 cggctggtac tcgctggacg aaggtgataa ccagcaagag cgtttcgcca gctatctcat
      481 tgccgccgtg caacaggcaa ccaacggtca ctgtgcgata tgtgagacga tggcgcaaaa
      541 acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc tggcggaatg
      601 gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc cagtgatcca
      661 cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg tggtgttgtc
      721 acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgatcaac tgctggaaat
      781 tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg attgccgtct
      841 gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt ccggttgggc
      901 gacggcacta cagctaatcg ccctctccgc ccggcagaat acccactcag cccataagtc
      961 ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg tcgatgaggt
     1021 tctggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca ttttgcgctc
     1081 aatgaacgat gcactcatca cccgtgtgac cggcgaagaa aacgggcaaa tgcgcctcga
     1141 agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg agtggttctg
     1201 ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac tggcggcgga
     1261 gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat ttcccagcga
     1321 agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata ttctgcttaa
     1381 tcacgcctgg agtctgttca accatagcga actgtcgctg ctggaagagt cgcttaaggc
     1441 cctgccgtgg gacagcttgc tggaaaatcc gcagttggtg ttattgcagg cgtggctgat
     1501 gcaaagccaa catcgctacg gcgaagttaa caccctgcta gcccgtgctg aacatgaaat
     1561 caaggacatc agagaaggca ccatgcacgc agaatttaac gctctgcgcg cccaggtggc
     1621 gattaacgat ggtaatccgg atgaagcgga acggctggca aaactggcac tggaagagct
     1681 gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg aagtgctgca
     1741 ctgcaaaggc gaattgaccc gctcactggc gctaatgcag caaaccgaac agatggcacg
     1801 ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg aaattctgtt
     1861 tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc agctgatcaa
     1921 cgagcagcat ctggaacagt tgccgatgca tgagtttctg gtgcgcatcc gagcacaact
     1981 gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg ggattgaagt
     2041 cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga ttcaatgctc
     2101 gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg aaaacctgct
     2161 ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc gggtgattta
     2221 ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata cggctaaacc
     2281 ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc gtgcacaaat
     2341 cttgctgggc gagtttgaac cggcagaaat tgttctcgaa gaactcaatg aaaatgcccg
     2401 cagtctgcgg ttgatgagcg atctcaatcg taacctgttg ctgctaaatc aactgtactg
     2461 gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa aactggcgaa
     2521 tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc aacaactgcg
     2581 ccagctgatt caactcaata cgctgccgga actggaacag catcgcgcac agcgtattct
     2641 gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga atttcgttga
     2701 acgtctgcta aatcatccag aagtgccaga actgatccgc accagcccat tgacccaacg
     2761 tgaatggcag gtcctggggc tgatttactc cggttacagc aacgaacaaa ttgcgggtga
     2821 actggaagtg gcggcgacca ccatcaagac gcatatccgc aatctgtacc aaaaactcgg
     2881 cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga tgatgggtta
     2941 tggggtgtga gtttagccgg ataatgcatt ggatccggct tacatcttta tatcattcaa
     3001 tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgtaccggc aagaaacgct
     3061 ccaccacggc gatattggtc agcagatggc atgaaggatg ggcgaccgta aattctcccg
     3121 cgcccgccag cgccatcggt agcaccagct ggtcggcgag atattccccc actgccgccg
     3181 ggcttgccag gtagcgtttc acctctttca ccaactgtgc cgcgaccacc tcggcactga
     3241 cgcgcttttc accgacgaca aaaaagcgtt cggtgatatt ttcactttcg acttcaagcg
     3301 agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgc tcatgcaggg
     3361 aaaaacttgc cgccagcgta gcgatttcac gctcagcgac atgccgcggc acgccagcca
     3421 gtaacacctc accacgcatc cgcacaatgt tcccgcgctc gccaagttgc aaggtgttaa
     3481 acgatgtcac gggcgagact tccgttgcta ccacgccgcc tccggcaggg taaaaaccgt
     3541 ggcgtaataa cgtggtttgc tgatggattc ccattttcgc cagcagcggc tccagcaccc
     3601 ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc acttcaacac
     3661 gcgatggccc atcggcaaac cacaacgcgg gcagcaccgt ttgcagcacc agcatacagc
     3721 ttccggcgct accgatagca aagcggtaat cgccaccgcg cacggtaccg ggccggaaga
     3781 ccagacgctg cgaccccagc tccgcgcctt ccaccgtcgc cccgcaaatt tccgtcgccg
     3841 cctttaccgc cgtcagatgc tggcgcaaca gtcccggttt tgcccgcccg gcacgaatgc
     3901 cggtgatggt aaatggctgg ccggttatca tcgacagact cagcgccgag cgcaggatct
     3961 gcccgccgcc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc atttatcctt
     4021 ttacacacac cacctgacgc agggtataga taacttccac cagatcgctt tgtgccgcca
     4081 tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg gcatctttac
     4141 ggcattctac atgcgcggtg gcacgaattt gatcttccac gctgaacagt tttttcgctt
     4201 tagtacggct cattacccgc ccggcaccgt ggctgcacga acagaacgac tcttcatttc
     4261 ccagtccacg aacgataaag ctttttgccc ccatcgaacc gggaataatt ccatattgac
     4321 cagcacgcgc agacaccgcg cctttacgcg tcacgtagat ctcttcacca aagtgctgtt
     4381 ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt tgtggctgtc
     4441 tgaccgtttt ctgcgtaatg ctctgcaacg ctgttaccac gttttccatc atcgcatcgc
     4501 ggttaaggct ggcaaaaagc tgcacccagg ccacggcttt caggtaatca tcaaagtatt
     4561 ccgtaccttc cataaagtac gccagatcac gtgacggcaa cgtctcaagt tggtcctgca
     4621 tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt ccgcgtgaac
     4681 cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca ataaagtggt
     4741 tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa cgcggatatt
     4801 tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg acgttaacag
     4861 gcgggttttc ccatgcgcct ttatcacgtt tacaacggcc agtggtacgc ccgtgcggca
     4921 ccgcagtttc aatcgcctga cgcagctccg ccaggttttc aggcagatct gccgccgtta
     4981 acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc gccggaataa
     5041 tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg tgtacatcag
     5101 gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata agttgctgac
     5161 gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca ttttcagtgg
     5221 tcagtaattc gtaattcatt ttgtttttct cttttcgttg gtatgtcctg ataattgcaa
     5281 ccgtcgtgcc agaaaattaa ataagcagat taatttttta attcattgtt ttaaaaaaga
     5341 ttatatcttt acgtccgtaa ccggagattt cccgcaaagc cgatttaccg ataatgaaat
     5401 atcgtctttt ataaggatat ctaagatgcg taaaacagtg gcttttggct ttgtcggaac
     5461 ggtgctggat tatgccgggc gcggcagtca gcgctggtcg aaatggcgtc cgacgctttg
     5521 tttatgccag caagaatcgt tggtcatcga tcgactggaa ttattgcatg acgcccgctc
     5581 gcgctcgcta tttgaaacgc ttaaacgcga tatcgccagc gtttcgccag aaacagaagt
     5641 ggtgagcgtt gagattgaac tgcataaccc gtgggatttc gaagaggttt acgcctgcct
     5701 gcatgatttc gcccgtggtt acgagtttca gccagaaaaa gaagactatt taattcacat
     5761 caccaccggc acccacgtcg cgcagatttg ctggtttctg ctggcggaag cgcgttacct
     5821 gcccgcccgg ttgatacaat cttcacctcc gcgcaaaaaa gaacagccgc gcggcccagg
     5881 tgaagtgacg attatcgatc tcgatttaag ccgttataac gccatcgcca gccgctttgc
     5941 cgaggaacgc cagcaaacgc ttgattttct taagtccggc attgccacgc gtaacccgca
     6001 cttcaaccgc atgattgaac agatcgaaaa agtggcgatt aaatcccgcg caccgatcct
     6061 gctaaacggc ccaaccggtg cgggcaagtc atttctggcg cgacgtatat ttgaattaaa
     6121 acaggcgcgg catcagttta gcggcgcgtt tgtggaagtg aactgcgcaa ccctgcgcgg
     6181 cgataccgcc atgtcgacgc tgtttggtca tgtgaaaggc gcgtttaccg gggcgcggga
     6241 atctcgtgaa ggattattac gcagcgccaa cggcggaatg ttgtttcttg atgagattgg
     6301 cgaactgggc gcagacgaac aggcaatgct gcttaaagcc attgaagaaa aaacctttta
     6361 tccgtttggc agcgatcgcc aggtgagcag taattttcag ctcatcgccg gaacggtgcg
     6421 cgatttacgc cagctggttg cagaaggcaa attccgcgaa gatctatacg cgcggatcaa
     6481 tctctggacc ttcaccctgc cggggctgcg ccagcgtcag gaagatattg aaccgaacct
     6541 ggattatgaa gtggagcgcc acgcctcact cactggcgac agcgtgcgtt ttaacaccga
     6601 agcgcggcgt gcctggctgg cctttgcgac atctccccag gcaacatggc gcggtaactt
     6661 tcgcgagctt tctgccagcg tcacgcggat ggctaccttt gccactagcg gacgcatcac
     6721 tctggaagtg gtggaagatg agataaaccg tctgcgctat aactggcagg agagtcgccc
     6781 ctccgcgctt acgcagctac tgggcgcaga ggcagaaaac atcgatctct tcgaccgtat
     6841 gcaactggaa cacgttatcg ctatctgccg ccaggcaaag tcgctttctg cagccggacg
     6901 acagcttttt gatgtttcgc gccagggcaa agccagcgtc aacgacgcgg atcggctacg
     6961 caaatacctg gcgcgttttg gtctgacgtg ggaagcattg caggatcagc acagctccag
     7021 ttgaatatgg tggtccgtca gcacctgcat cacgcttact ggcggcgggg catcggtgta
     7081 gacggcatct accatgctga tgctgcccat attgaccatc gcgttacggc caaatttcga
     7141 gtgatcgaca accagcataa cgtggcgcga gttctcaata atggcgcgtt tggtgcgaac
     7201 ttcgtggtaa tcgaactcca gcagcgagcc gtcgctatcg atgccgctta tccccagaat
     7261 gccgaaatca aggcggaact gggagataaa atcgagcgtc gcttcgccaa tgatcccgcc
     7321 atcgcggctg cgtaattcgc caccggcgag aataatgcgg aaatcttctt ttaccatcaa
     7381 cgtgttagca acattgagat tgttggtgac aatacgcaaa ttgctgtgat taagcagcgc
     7441 gtgcgccacc gcttccggcg tggtgccaat atcgataaac agcgtcgagc cgttggggat
     7501 ttgctccgcc actttacggg cgatgcgctc tttttcttcg gtctgggtgg ccttgcgatc
     7561 gtgccacggc gtgttaaccg aactggaagg cagcgccgca ccgccgtgat ggcgcaggat
     7621 caggttttgc tccgccagct cattgaggtc gcggcgaata gtctgcgggc tgacggagaa
     7681 atgctctact agctcttcgg tactgacata accctgctgt ttaaccagtt cgataatacc
     7741 gttgtgacgt tgtgtttgtt tcatttctaa atccctggaa ttattttcgt tttcgcgcat
     7801 tgagcgaatc aacaaaagcc atcgctaaac ccacggctaa cccggcgatg tgtgctccgt
     7861 tcgccatcga catcccaaac aaatcaaacc atccggcgac aatccagatc agcgcaaaga
     7921 taattaaccc acgttgcagg taaatgccac tttgcggatc gcgttcgcca cgtagccaga
     7981 cgtagcccat cagcgcatac accacgccag aaagcccgcc aaaccacggc ccgctgaatt
     8041 tttgctgcac atagccgctt aacagggcgc tgataagcgt aatgacaatt agcttaccgc
     8101 taccgaggcg tttttccacc gcaccgccga gataccacca ccagagcagg ttaaagagga
     8161 tatgcatcag cgagaagtgc attaacgcgt gggtgaagta acgccagaac tcaaatttca
     8221 gtgttggatc gaatggccag gccagccata acatcacttc ctgatcgccg agaatttgca
     8281 tggcaataaa caccaccacg caggcgatca tcatcaccca ggttaccgga cctgcgcgtt
     8341 cacgcaaggc ggcaaagaaa ggataacggc gataatgcag gccactgccg gtatggcctg
     8401 cctgccagct cgccgccaga taacgcggat ctgccggatt ttcgagaaaa cgcgccagct
     8461 ccgcccgtac gcgctcggcc tgggactcat ccgccagcca gacatcgctt tggttatgtt
     8521 gttgaatcgt gaggataaca ccctgcgtcg ccatgtaatc aacaaacgcc tgcgccacgc
     8581 gggggttagc aaaagaggta atcatcaaca tcgttgctgt cgcttattcc acacaaaagg
     8641 ggacagtata aagcgttacg cgccgtacgc cacctctgcg ggaaactgac gttgccaggc
     8701 ttcaaagccg ccatcaatgc tatagaccac atcgtagccc tgttgcagca gatactgcgc
     8761 cgcgcctttg ctgctattac cgtggtaaca catcaccatc accggtgtgt caaagtcgtt
     8821 atcacgcata aacgcgccca gtgtgtcgtt ggttaaatgg aaagcctgca ccgcatgccc
     8881 cattgcgaaa ctctgtggat cgcgaatatc gaccagcacc acctcttttt cctgcaactt
     8941 ctggtgcgcg tcggcaacgt taatacattc gaactgatcc atgcgtctct ctttctttac
     9001 aaacaagtgg gcaaatttac cgcacagttt acgtcgaagc ggcagataaa cgccataatg
     9061 ttatacatat cactctaaaa tgttttttca atgttaccta aagcgcgatt ctttgctaat
     9121 atgttcgata acgaacattt atgagcttta acgaaagtga atgagggcag catggaaacc
     9181 aaagatctga ttgtgatagg tggcggcatc aatggtgctg gtatcgcggc agacgccgct
     9241 ggacgcggtt tatccgtgct gatgctggag gcgcaggatc tcgcttgcgc gacctcttcc
     9301 gccagttcaa aactcattca cggtggcctg cgctaccttg agcactatga attccgcctg
     9361 gtcagcgagg cgctggctga acgtgaagtg ctgctgaaaa tggccccgca tatcgccttc
     9421 ccgatgcgtt ttcgcctgcc acatcgtccg catctgcgcc cggcgtggat gattcgcatt
     9481 ggtctgttta tgtacgatca tctgggtaaa cgcaccagcc tgccgggatc aactggtttg
     9541 cgttttggcg caaattcagt gttaaaaccg gaaattaagc gcggattcga atattccgac
     9601 tgttgggtag acgatgcccg tctggtgctc gccaacgccc agatggtagt gcgtaaaggc
     9661 ggcgaagtgc ttactcgtac tcgtgctact tctgctcgtc gcgaaaacgg cctgtggatt
     9721 gtggaagcgg aagatatcga taccggcaaa aaatatagct ggcaagcgcg cggcctggtt
     9781 aacgccaccg gcccgtgggt gaaacagttc ttcgacgagg ggatgcatct gccttcgcct
     9841 tatggcattc gcctgatcaa aggcagccat attgtggtgc cgcgcgtgca tacccaaaag
     9901 caggcctaca ttctgcaaaa cgaagataaa cgtattgtgt tcgtgatccc gtggatggac
     9961 gagttttcca tcatcggcac caccgatgtg gaatacaaag gcgatccgaa agcggtgaag
    10021 attgaagaga gtgaaatcaa ttacctgctg aatgtgtata acgcgcactt taaaaagcag
    10081 ttaagccgtg acgatatcgt ctggacctac tccggtgtgc gaccactatg tgatgatgag
    10141 tccgactcgc cgcaggctat tacccgtgat tacacccttg atattcatga tgaaaatggc
    10201 aaagcaccgc tgctgtcggt attcggcggt aagctgacca cctaccgaaa actggcggaa
    10261 catgcgctgg aaaaactaac gccgtattat cagggtattg gcccggcatg gacgaaagag
    10321 agcgtgctac cgggtggcgc catcgagggc gaccgcgacg attatgccgc tcgcttacgc
    10381 cgccgttatc cgttcctgac cgaatcgctg gcacgtcatt acgcgcgtac ttatggcagc
    10441 aacagcgagc tgctgctcgg caatgcggga gcggtaagcg atcttgggga agatttcggt
    10501 catgagttct acgaaacgga gctgaaatat ctggtcgacc acgagtgggt ccgccgcgcc
    10561 gacgatgccc tgtggcgtcg cacaaaacaa ggtatgtggc taagtgcgga acaacaatct
    10621 cgtgtgagtc agtggttggt ggagtatacg cagcagaagc tgtcactggc gtcgtgattt
    10681 aatgtaaggt ggccaggtca gatttcaatc tgacctgaga ctgatgacaa accaaaaaac
    10741 tgccagatgc gctacgctta tctggcctac gttgtttata caatatattg aatttgcatg
    10801 attttgtagg cctgataagg cgttcacgcc gcatcaggca tgaacaaagt gcactttgtc
    10861 aacgataata caagcagcca gcgcaaccac cgtcggccac accaacaacg gcacaataac
    10921 aaagccaaga aataacggat aaatataaag tctgattata ttcatcatta ttgtcctgat
    10981 gattcggggg atgcttcata cagcggcaca taccccacat cctctaccaa ctgttgcccc
    11041 tgcggactta aaaaccagtc aacaaatttc tgcgtttccg gcgtgggatt ttcacgcgtc
    11101 accatatagg catccacaat gtatggataa gtgccgttgc ggatattttc ctcggtaggc
    11161 gcaacgccat ttatagccaa aagtttaatc tgcttgcgca ttttgagcat attgtcattc
    11221 atacgagtga cgtaatagtg gaaggtgtac ccaatggaag atggcgtgtt ttgatagtcg
    11281 gcaacttcag taattaaacc gcccataaga tcaatcacag tggatttttt agctggcagc
    11341 attggggtgt cttgcataac caaccccttc ataatcgttt ggcttccaga atcttcaggg
    11401 cgctgccaga cttttatatg ttcgtcacta ccacctactt tattccagcg gctagttttg
    11461 ccgctaaaaa tatctttaac ctggtgctca gagagagaat ttaccgggtt attaatatca
    11521 acgataaaga caaaggcttc gcgggcgaaa gcggtatatt tcagtttaac acccgcttct
    11581 tttgcacgtt ttttctgtcc ttccgacggt tgtgcaacaa aaataaccgt cgcggaacct
    11641 ttaatcagac tatcgtaggc ttcagctgtt cgggtttgct gcaaaccata cgcctgccag
    11701 gccagcgagg ttcgatcctt atcttttggc ttgggtacta atttataata cgcggaggca
    11761 tacagcgggt ataaggctgt agcgccgtac actctcggcc atcttgacca atcatcgctg
    11821 taacgtaatg caggttgccc ccgcagagac gtcagtccat ggctctcttc ttccctgacg
    11881 ctgttatagt acccttcccg gtcgacacat cccgccagta acgccgtgcc taatagtaaa
    11941 acaccggtga atttattcat ctcgctgatt cctttctctc gagcgttatt ccattgttgg
    12001 atacagcggc acatatccca catcttccac cagactctgc ccctgcggcg ttaaaaacca
    12061 ttccaccaat ttttgtgtct ctgacgtcgt attttcccgc gttaccataa aggcatcgac
    12121 gatataggga tatttgccgt tgcgaatatt ttccgccgtc gatgtaatac cgttaatcgc
    12181 tagcagtttt atatttttat cggcgttcat ttgtgttgcg taataacgga aggtgtaacc
    12241 tattgcgttg tttgtattac gatattcggc aacgacttta atcattccct tcattacgct
    12301 tgccacttcg gtttcctgcg gcgagatcat acggacattt ttcatgacct gcgattgcat
    12361 caccgtctgg ctgccagagt cttccgggcg ttgccaggtc tggatctcct gatcgttacc
    12421 gccaacagta tgccagttgg taattgcacc actgaaaatg tcacgcacct gttgttcagt
    12481 cagggaatta accggattat ccgcattgac gatgaaaacg aatgcttcac gggcaaaggg
    12541 ggtgtacagc aaagtgacac cagattcctt tgcacgtttt ttttgcccac cggaaggttg
    12601 cgccacgaaa ataatatcag catcgccctt aacgattttg ttatatgctt ctggcgtacg
    12661 agagttttcc agataatccc aaacgtgaaa atcctctggt attacactta atgcataaaa
    12721 tgcagaagca taaattgggt acgcagccgt ggcgccatcg attcgcggcc agttttgcct
    12781 aaactgaatt tgcggtttgc cacgcagcgg cgtcagtcga ttattgagtt tatcgggtcg
    12841 ccaggcccag atatcaatat tttcgcgtac accgacacca ggattttgct gcttatataa
    12901 atgggcctga tacccggcga caataccaag taagagaagt agaaacagga gtaagttacg
    12961 gcaacgtagt ccggacgtgc cggataaagg atatctggtc ggccaggcgt atcccagcga
    13021 aaaactgata tgcgccccaa cgggaataat gaccatccac cagtactcac ctccgactac
    13081 tgagactaac aaaacgccca gccaggggag cataagatca gccaaccaac ccgatcccat
    13141 ctggtgaaat tgataatctg ccagatgcag cgcgattatc cagccaacca gggttaatag
    13201 cgcgggtaag agaaaaaagc attgccgcca gacggctatt tctctgtttt ctggcgcgct
    13261 atcaccggcc cacacgcgac cagagaaaaa tattattatc gcccccagcg tcagcataaa
    13321 gacgttgagt agaaggtaaa ttgttggcgt gataaggata ttacaaactt caataatacc
    13381 ggcaagtccg acacccagca tagcaacaac cgctgccaaa aattgcgcca gtatggggat
    13441 gccgaaaaaa gtcattatca gcgaggtcaa aatccatttt ctatttggca tcattctgtc
    13501 cattcttcct gaatgacgga attatactcc ccgagaaccc tcgccccctc tggacacttt
    13561 tccgaaataa aggcgaaaaa aaacgggacc ttgtggcccc gttctgtttt ttagcgaact
    13621 tacaatctca ccggatcgat atgccagata tgatcagcgt actctttgat agtacggtca
    13681 gaagagaagt agcccatatt ggcaatgttc agcatcgctt ttgcggtcca ctcttcctga
    13741 agctcgtaga gttcatcgac tttatcctga caatcgacat agctgcgata atccgccagt
    13801 acctggtagt gatcgccgaa gttgatcagc gaatcaacca gatcgcgata gcgacccgga
    13861 tcttccggac tgaatacacc gctgccgatt tgcgtcagca cctgatgcag ctcctcatct
    13921 ttctcgtagt attcacgcgg tttgtagccc tgacgacgca gttcttccac ttcttccgct
    13981 gtgttaccaa aaataaagat attgtcagca ccgacatgat ccagcatctc gacattcgca
    14041 ccgtccaacg taccgatagt cagcgcaccg ttaagcgcaa acttcatgtt actggtgccg
    14101 gaagcttccg tccctgccag cgaaatctgt tcagacagat ctgccgccgg aatgatcaac
    14161 tgcgccaggc taacgctgta gttcgggatg aacacgactt tcagcttatc gccaatctgc
    14221 ggatcgttgt tgatcacttt cgctacgtca ttgatcaaat ggataatatg cttcgccatg
    14281 taataggccg aagccgcctt accgccaaaa atattcacgc gcggtaccca cttcgcatcc
    14341 gggtcggcct tgatgcggtt atagcgggta atcacatgca acacattcat caattgacgt
    14401 ttgtattcgt gaatacgttt gatttgtaca tcgaacaacg cctttggatt caccaccaca
    14461 ttcagctgct gggcgatata ctctgccaga cgctttttgt tctccagctt cgcctgatgc
    14521 acagcgtgat taaccattgg gaaatcacag tgttgttgca gctcattaag caggctaagg
    14581 tcggtgcgcc agttacggcc caggtgttcg tccagcacgg ctgaaagcga tgggttcgct
    14641 accgccagcc agcgacgcgg cgtcacaccg ttggtgacgt tggtgaaacg acccgggaag
    14701 attttcgcaa agtcggcaaa caacgattgc accatcagat tagagtgcag ttccgataca
    14761 ccgttaactt tgtggctcac aacaaccgcc agccaggcca tacgcacacg acgaccgttg
    14821 gattcatcaa tgatcgacgc ccgtcccagc aggtcggtat cgttcggata ctgttcctgc
    14881 aaggttttca ggaaatagtc gttgatttca aagatgatct gcaggtgacg cggcagaatt
    14941 ttacccagca tatcaaccgg ccaggtttcc agcgcctcgc tcatcagcgt gtggttagtg
    15001 taggagaaga cctgacaaca cacctcaaac gcgtcgtccc agctaaattg gtgctcatcg
    15061 atcagcagac gcatcatctc aggaatcgac agtaccggat gggtatcgtt gagatgaatc
    15121 gcgattttat ccgccaggtt atcgtaggtt ttatgcaact gataatggcg gcttaaaatg
    15181 tcctgaatgg tcgaggaaac caggaagtat tcctgacgca ggcgcagctc acgcccggag
    15241 taggtggagt catccggata cagtacgcga gatacgttct cggagtggtt tttatcttcc
    15301 actgccgcga agtagtcacc ctggttgaat ttaccgaggt taatttcgct actggcttgc
    15361 gcactccaca aacgcagcgt gttggtcgcg tcggtgtcgt aacccgggat tatctgatcg
    15421 taagcgactc ccagaatctc ttcggtttca atccagcgcg tttttttacc ttcctgctga
    15481 atgcgaccac caaaacggac tttatagcgc gtgttgtggc gtttgaattc ccacgggtta
    15541 ccgtattcta gccagtaatc aggcgactct ttctggctac cgttaacgat gttctgcttg
    15601 aacataccgt agtcatagcg gatgccgtaa ccgcgtcccg gcaaccctaa cgtcgccaga
    15661 gaatcgagga agcaagccgc cagacgtccc aggccaccgt taccgaggcc cgggtcattt
    15721 tcttcatcaa tcagctcttc gagatttaac cccatcgctt ccagtgcgcc ctgtacatct
    15781 tcgtaaattc ctagcgacaa catggcgttg gagagcgtac ggccaatcaa aaactccatc
    15841 gacaggtagt aaacctgacg agtttcttgc gacaactggg cacggtttga acgtaaccag
    15901 cgctccacga gacgatcgcg cacagcaaat aacgttgcgt tcagccattc atgtttattg
    15961 gcgacgaccg ggtcctttcc aatcgtaaac atcagcttgt aagcgataga gtgcttaaga
    16021 gcttctacgc taagcgtggg cgatgaatat gtaaacggag cattcatata ggcgtttcct
    16081 gaaaactatt tcaagcgata gtaaagctca cggtacgact tcgccgcgac ctgccagcta
    16141 aaatccattg ccatagcctg acgttgcaca aaccgccaca gtgaaggacg ggaccacagt
    16201 acaaaagcac gtcgaatagc ccgtaacagc gaccaggcat tactatcttc aaagacaaac
    16261 ccactggcga cgccatctgc aaggttctca agagaacagt cagaaaccgt atcagcaagc
    16321 ccaccggtgc gccgcactaa cggcagcgta ccgtacttca atccataaag ttgcgttaag
    16381 ccgcacggtt caaaacggct gggcaccaga atgacgtccg cgccgcccat aatgcgatgc
    16441 gaaaatgctt cgtgatagcc aatctgaacg cccacctggc cggggtattc cgctgccgcc
    16501 gcaaggaaac cttcctgcag caccggatcg cccgcgccga gtagcgccag ctgcccgccc
    16561 tgctccagaa ggcccggtaa ggcttccagc accaggtcga gacctttctg gctggtcaga
    16621 cggctcacca ctgcaaaaag cggcacttta tcgtcaacct taagccccat tgcgatttgt
    16681 aactggcgct tattttccgc tttatcttcc aacgtatcgc gggtgtaacg cgaggccaac
    16741 agtaagtccg tctctggact ccagattttc tcgtccacgc cgttcagtac gccggaaaga
    16801 cgcccttcac ggtgacgctg ttgcaacaga ccttccatac cgtaggcaaa ctgcggttcg
    16861 gtgatctcgc gagcgtaggt tggactgacc gccgtaatgt gatcggcata gtacagaccg
    16921 gccttcagga aagagatttg tccgttgaat tccagcccat gaatattaaa gaatgaccat
    16981 ggcaattgga tgtcattcat gtgatgtgca taaaacatgc cttgataggc caggttgtgc
    17041 acagtaaaca ccgacttcgc cggacgcccg cgcgccgcca gatacgcagg cgcaaggcct
    17101 gcatgccagt cgtgcgcatg caccacatca ggacgccaga atgggtcaag cccgctggcc
    17161 atttctgccc caacccaccc cagcagcgca aaacgcaata cgttgtcggt ataggcaaat
    17221 aagttggtat cgtgatacgg gcttcccgga cgatcataga gatgcggcgc gtcaatcagg
    17281 tatatgccaa ccccgttgta atgaccgaac aacagcgtga tatgcccggc gaaggtatca
    17341 cgacgggata ctacctgcgc atcggtcacg ccacggcgaa tatcgggaaa tgcaggcaac
    17401 agtacgcgag cgtcaacgcc gtctgcgatt tgtgctgcgg gtaatgcccc aataacatca
    17461 gccagaccgc cggttttaag cagcgggaac atctctgaac atacatgtaa aacctgcatt
    17521 atcgctcctg tttatgccct aacttccgta gcatttcgcg cgttaccagc acgatgcctt
    17581 cttctgaacg atagaaacga cgtgcatctt cctctgcgtt ttcaccaatc accatgcctt
    17641 ccggaataac acaagcacga tcaatgacgc agcggcgcag acggcacgag cgacctaccc
    17701 atacttccgg taacaatacg gcggaatcaa tgttgcagaa tgaattcacg cgaacgcgcg
    17761 agaacagaac ggactgcacc accaccgaac cggagatcac acaaccgccg gaaaccagtg
    17821 agttaagggt catcccgtgg ctaccggagc gatcctgcac gaatttcgct ggcggtaatg
    17881 attcattgta ggtgcgaatt ggccaattgc gatcgtacat atccagctcc ggcaccacag
    17941 aggccagatc gaggttcgct ttccagtaag cttccagcgt acccacatcg cgccagtacg
    18001 gctcggcatc cgggtcggat tgtacgcaag agagcgggaa cgggtgcgca taggccagac
    18061 cggcttcggt gatcttggga atcaaatctt tgccaaagtc gtggctggag ttctcatcgc
    18121 gatcgtcttc ttccagcagt tcatacagat agtcggcgtc aaagacgtag atacccatac
    18181 tcgccagaga tttgctcgga tcgttcggca ttgacggcgg gttagcaggt ttttcaacga
    18241 attcgataat tttatcgttc tcatcaaccg ccataacgcc aaatgcggag gcttcttcaa
    18301 tcggtactgg catacaagca acggtgcaac gtgcgccttt ttcgacgtga tcgataagca
    18361 tacgcgagta gtcttgcttg tagatatggt cgcccgccag gatcaccacg tattccgctt
    18421 tataacggcg gataatgtcg aggttttggg tgaccgcatc tgcggtgccg cgataccagt
    18481 tttccccttt cattctctgc tgtgctggca gcagatcgac aaactcgttc atttcttcat
    18541 tgaagaatga ccagccgcgc tgaatgtgct gcaccagagt gtgggactgg tactgggtga
    18601 tcacgcccat acgacggatc ccggagttga tgcagttaga cagcgcaaag tcgataatgc
    18661 ggaacttacc gccgaagtgt acggccggtt ttgctcgctt attggttaaa tccttcaggc
    18721 gggtaccacg tcctcccgcc agtatcaggg caacagattt caatggcagc tggcgcgcca
    18781 acattaagtg atcgttcttc tctaaactaa ccatgactaa ctcctttttt atcatctctg
    18841 gaacacacac aatccgtgtg caggtccctg ccagacagcc gtaatcactg ggttatcctc
    18901 tccagcgaat gggggaatgg cgtgccactc cccagcaggt aaaacaatct ctgttacctc
    18961 aagcgtggcg ttaattgcga tcaaaaagcg atccgagagc agaatttgca gctgtttcgg
    19021 cccgttttgc cactcatccg tgcttaaagg ttgagcatat cgatttagcc aacggacatt
    19081 gccgtcgcct tcttcccacc agcgattctc caccaaagcg ggaatacgct tgcgcagatg
    19141 gattaacgcg gcggtaaatg cggttaaacc actgcttgcc tgcgaccagt ccaaccaggt
    19201 taattggtta tcctgacagt aggcattgtt attgccatgc tggctgtgac catgttcgtc
    19261 accggccagt aacatcggcg tgccctggga gagcaacaac gttgttaaca gggcatgaat
    19321 gctgtcgcgc cgccgttcaa ccagatcaag agtaccgcct aacccttctt taccatgatt
    19381 gttactgtaa ttgttgttgg tcccgtcgcg attttcttct ccgtttgctt cattgtgttt
    19441 atggttgaag caaacgcagt cgcgaagcgt aaaaccgtca tgtgcggtga cgagattaat
    19501 cgcggcactc ggcagacgac cattacgttt aaaaacatcg ctggaggcag caaaacgccc
    19561 ggcaaacgcc cccagaggca aatcataatg cagccagaaa cgacgggcag catcgcggaa
    19621 atgatcgttc cactcggcaa aaagcggcgg gaaatttccc acctgataac caccaggagc
    19681 gatatcccac ggttcagcaa ttaacttcac ctgcgagagc accgggcagt tctggatagc
    19741 ggtaaacaac ggcgcatcct gacggaactc tggcgtacgg cccatgactg ccgccagatc
    19801 aaagcggaaa ccatcgacgt ggcaggtttc tacccaataa cgcaggcagg cgctggcata
    19861 atccaccacc gccgggtgac tcaaattgag cgtgttgcca caaccggtcc agttgtgata
    19921 atcgccgtct tctcttatcc aataatagct acggttgtcg atcccacgca gcgagaataa
    19981 cgggccgtcg aggtccagtt ccgcactatg gttgagcacg atatcaagaa tgacttcgat
    20041 acccgcttta tgcagtgctt tgattgcatc gcgaaactca tccagcgccg tttctggcga
    20101 gcaggcatac gccggatgca gcgcaaacat cgccaccggg ttgtaacccc agtagttact
    20161 tagtcccatg cgttgcagac gtggttcact ggcaaactgc gccaccggca gcagttccag
    20221 cgcggtaatg cccaactgtt tcaaatagtt gatcatcacc ggatgcccga gggctttata
    20281 agtgccacgg atctcgaccg ggatctccgg gtgcaggtac gttaatcctt tgacatgggc
    20341 ttcataaatg atggtgctgc cccacggcgt gcgcggcggg gcatcatctt cccagtcata
    20401 gtgatcaacc accactacgc atttcggcgc aatggcggcg ttgtcgcgat agtcaggttc
    20461 attatgaccg gcgtgcagca gcgggttatc tttaaactcc ccgtcaattt gccgcgcgca
    20521 aggatcaatc aacaacttcg ccgggttaaa gcgatgcccc tcggcgggtt gccaggggcc
    20581 atgaacgcga taaccataac gcaaacccgg gcgcgcatcc ggcagataac cgtgccaaat
    20641 gtcgccactg tgccctggca agtcatagcg atgttcctgg ccattggcgt caaagacaca
    20701 cagctctacc cgctcggcat gagcggagaa aagtgtgaag ttgacgccct gaccgtcgta
    20761 atgcgcgccg aggggagcgg gcttgccaat ggcgagttgt gtcattctgc ctcccgaacc
    20821 agccagatag tggccagcgg tggtagcgtc aggcttagtg aatgctgacg accgtggctg
    20881 gcaatctcat cgctgtgtac cgtgccgcca ttgcctgcat tactgccgtg atagtgcatg
    20941 gaatcggtat tgaggatctc acgccatttg cctggctgat taatgccgaa gcgataatca
    21001 tgacgcggca ccggcgtaaa gttactggca acgatgattt cgttaccctc tttatcgcga
    21061 cgcacaaaga tcagcaccga gcgttctttg tcatccacca ccagccattc aaagccgtac
    21121 gggtcaaaat ccagttcatg cattgcttta tggtggcggt aggtgaggtt cagatcgcgc
    21181 accagacgct ggacaccgtg gtgccagtta tcgccgcctt ccaacagatg ccagtcgagg
    21241 ctggcgtcat ggttccactc gcggccctgg gcaaattcgt tccccatgaa cagcagtttc
    21301 ttgcccggaa atgcccacat ccagccatag taagcgcgca ggttagcgaa tttctgccat
    21361 gcgtcgcccg gcatccggtc gagaatcgat tttttaccgt ggaccacttc atcatgcgac
    21421 aacggcagga cgaagttttc agtgtagttg tagagcatcc cgaaggtcag tttatcgtga
    21481 tgatactgac gataaatcgg gtcgagcttc atgtagtcca gggtgtcatg catccagccg
    21541 aggttccact tgtaccagaa gcccagaccg cccatatcct gcggacgaga aacgccaggg
    21601 aaatcggtag actcctcagc cattgtcacc gcaccggaaa cctgctcacc aagaatacgg
    21661 ttggtattac gcaagaattc aatcgcttca agattctcgc gcccgccaaa ttcgttcggg
    21721 atccactccc cctctttacg gctgtagtcg cgataaatca ttgacgccac cgcatcgacg
    21781 cgcagcgcat caataccaaa acgttcaatc cagtaaagcg cgttaccgac aaggaagtta
    21841 ctgacttcac ggcgaccata gttgtagatc agcgtgttcc agtcctgatg atagccttca
    21901 cgcggatcgc tgtgttcata caagttcgtg ccatcaaatt cggcaagcgc aaagtcatca
    21961 gtcgggaagt ggcctggcac ccagtcgaga atcacgttca gaccagctgc gtgtgcggca
    22021 tcaatgaaat aacggaagtc gtcgcgagta ccaaaacggc gggtcggtgc atacaggccg
    22081 gttggctgat aaccccaact gccatcgaag ggatgctcgt taatgggcag tagttcgagg
    22141 tgggtaaagc ccatccattt agcataaggc accagttgat cggccagctc gcggtagctc
    22201 aaccagaaat tgttgtcggt gtggcgacgc caggagccca ggtgaacttc atatatagag
    22261 attggcgcat caaactgatt cgcttttttg cgctcttcag tctgtacaac cttttccggc
    22321 agcccgcaaa taagagacgc ggtttccggg cgcatttgcg cttcgaaggc ataagggtcg
    22381 gacttcagac gcaagttgcc attggcatca atcatctcgt atttatagag ctgaccgtta
    22441 tgcgccccag ggataaacag ttcccagatg ccgctctctt tacgcaggcg catcgggtga
    22501 cggcgaccgt cccagtagtt gaattgccca accaccgaga cccgacgggc gtttggagcc
    22561 cagacagaga aacgcgtacc tgtgacgcca tccatagtat ctgcatgcgc gcctaaggtt
    22621 tcatacgggc gcaggtgagt accttcagat aatagccagg catccatttc ctggattagc
    22681 ggaccaaaac ggtaaggatc atcaatcagg ttttgctgac catgccagac aacagccaac
    22741 tgatagcgga aaaaattctt acgtcgcgga atgacgccac taaagaatcc ccgtgagtcg
    22801 agacactcca gttttgcgag tttgcgcccg gttttcggtt caatcaccca cacatcggta
    22861 gcgtcgggta aaagggcacg gacttccagt cccgcggtgg ttttatgcat tcccagtacg
    22921 gaaaaaggat ccgcaaaatg gcctgcaatt agcgcgttaa tcacgtctct atcgatacga
    22981 tcggacatgc ttgtcttcct gttttattgt gtcaccccat ccaattggat ttttgacttc
    23041 tggttgtgac atttttttga cctgaacggc gcagcactct gtgcatcctc tctgcgtcgt
    23101 cctcacttca ggtaaggctg tgaatactca tgtattcagc cacccttaaa gaatagccaa
    23161 tgctctattt aactcccggt aaatcatgaa acatctgcgc ttactcctgt attacgcact
    23221 aacaggggcg gcatcgcgcc ccagatttaa tgaataaaga ttacgccagt tgacgaagca
    23281 tccgacgcag cggctccgcg gccccccaca gcagctggtc gcccacggta aaggctgaca
    23341 ggaactctgg tcccatattc agcttacgca ggcggcctac cggcgtggtc agcgtgccgg
    23401 taacggcagc tggggttagc tcacgcatag tgatttcccg atcgttcgga acgactttcg
    23461 cccacggatt gtgcgcagcc agcaattctt ccacggtcgg aatcgacaca tcttttttca
    23521 atttaatagt gaatgcctgg ctgtggcagc gcaatgcccc gacacgcaca cataaaccat
    23581 ctaccggaat tacggaagat gtgttgagga tcttgttggt ttccgcctgc cctttccact
    23641 cttcgcggct ctgaccgtta tcgagctgtt tgtcgatcca cggaatcagg ctacccgcca
    23701 gcggcacgcc aaagttatcc accggcagct caccgctacg ggttaaggtt gtgactttgc
    23761 gttcgatatc gagaatagca gaggacgggg tcgcgagttc atctgctaca tggccataca
    23821 gatggcccat ctgggttaat aactcacgca tatgtcgcgc accaccgccg gaagcggcct
    23881 ggtaggttgc aacggacacc caatcaacaa gatcattggc gaataaacca cccaacgaca
    23941 tcaacatcag gcttacggta cagttaccgc caacaaaagt cctgatgcca ttatttaatc
    24001 cgtcggtaat gacgtcctga ttgacggggt caagaataat gatggcgtca tctttcatgc
    24061 gcagagacga tgctgcgtca atccagtaac cctgccatcc gctttcacga agctttggat
    24121 agatttcgtt ggtataatcg ccgccctgac aggtcacaat gatatcgagg gcctttagcg
    24181 cctccagatc aaaggcatcc tgaagtgtgc cagtggttcc gccaaaagac ggcgcagcct
    24241 gaccaagctg agaagtagaa aagaagacag ggcgaatggc gtcgaagtcg cgctcttcaa
    24301 ccatgcgttg catgagaacg gagccgacca taccgcgcca gccgataaaa ccaacatttt
    24361 tcataagcgt tttttcctgc aaagatgtgt gctgtataaa tgtgccggtc tcctcttggc
    24421 acatctttca ccatacaaaa agcagccaaa gtcgcaagtg aaattaatca atgatagcga
    24481 agccatcagt aatgcgactt atcctgcttt gttagcacgc agaaagtccg cggcaattat
    24541 cagggaattt gagttatgaa tgaaatcatt tctgcagcag ttttattgat cctgattatg
    24601 gatccgctcg gaaacctacc tattttcatg tccgtactga aacatactga accgaaaaga
    24661 cggcgggcaa tcatggtgcg agagttgctt attgctctcc tggtgatgct ggtgttcctg
    24721 tttgcgggtg agaaaattct ggcatttctc agcctgcgag cagaaaccgt ctccatttct
    24781 ggcggcatca ttctgtttct aattgccatt aagatgattt tccccagcgc ttcaggaaat
    24841 agcagcgggc ttccggcagg tgaagagcca tttatcgtgc cgctggcaat tccgctggtc
    24901 gccgggccaa ctattctcgc cacgctgatg ttgttgtctc atcagtaccc gaatcagatg
    24961 ggacatctgg tgattgctct gctgctggcc tggggcggca cctttgtcat cctgctacag
    25021 tcttcgctat ttttacgtct gctgggcgag aaaggggtga acgcacttga acgcctgatg
    25081 ggattgattc tggtgatgat ggcaacccag atgttcctcg acggcattcg aatgtggatg
    25141 aaggggtaag gttcaaacag gtgccgcaca caaccagacg cggcacctgt tttattacct
    25201 gaactgataa tgaccttcgc ccaatcggat aacccttccc tcttcttctg cgcggttcag
    25261 ccacacacta atttgttcga tggttaaacc agtacaagac gccagccttt caatacttat
    25321 cgattccttt gcaaggatcg ccagttccgc cacaaagagt tgataaaaat cgacaggcgt
    25381 atacgcgggc attactgcgt aatttgcgtc atcttcatgt agcgaaaaca gttctggttg
    25441 agcgttacgg agtgctctgt catacatcag cccaggaacg tctggcagtc tgatcgtcac
    25501 attctcagcc tgttcagcac tccatgacgc cccattttct actaattgtg aattggctga
    25561 ttgcttatcc tgatttggtt taacctgcac aggcagccac tgatgtttta atgcctccat
    25621 cgccccggtt atcgttccac cggtcattcc cgcacgtaca accattgcgc tttcagcaag
    25681 gcaataaata tatttatttc gcgccatcgc attgccgacg gtgaaacgta cctctgggta
    25741 aaacggcgaa atcaacacca ggttgcctgc tataagccct tcacgccatt tcactaacgt
    25801 actcgttttt aacaagctat cagctaatac gccaacggca gttcccccgg cctccagtgc
    25861 cgacgccatt gcacattcat caatacctcg cgcaccacca gagataacgc aaatcccctg
    25921 ttgggccagt ttcgcggcca gttgttgggt atagcgcaaa tcgtcagtcg gagcatctcg
    25981 cgagccaaca atcgccatac cttccgcttg caataatgct ttattcccgc agccaaataa
    26041 aacgggaggt gcatccgttc gcaatcggtt tttcaagcga acgggataat cagcatctcc
    26101 gcgggttaag atccataaac cggcgcgttg ccacttatct accgccaacg ccagactatg
    26161 accacgcgcc agcaagccaa gaatacgctc ctgagaaata cgcggatcgc tccagcctgt
    26221 aagtttctct tgcggttgcg gcaccagaag ctcggcggga ctgatacgtt ggtgcttcag
    26281 ccataatgca aacttccccc attcactatt actaagaggt ttatactcac tcgccgccgc
    26341 gcgagaaaaa tcgctggtta gcaggagagt tgcttgtgca ttggctgaaa gattcatcaa
    26401 ttttttaccg aggtagaagc aagggcaaga ggataaaccg tcgggcaacc tgcctggcgt
    26461 aacagtgctg tcagaactgt cagcgtccat gcagaatcca cgatatcgtc aaccagcaac
    26521 gccggaccag gcatcaaagg aggggtaata acaaacgccc cgtcgagatt ttgacactgg
    26581 tggaaacggt tttgctgcat tttctgcggt ggattgtcca cgactttttc aatggcatca
    26641 ataaaaggta agccaagttt cgccgccagt cgccgggcaa aatcaggaac caggtcgagg
    26701 tgctttaatg aaggaacgca gcatacccag gttggttcag gatgcggatt ccagcgttgg
    26761 cgaaccattt ccgcacatgc ctccaccagt tcatcactga atcgccccgt ttttttctcc
    26821 tgtgctacct gctttcccca cccggaatct ccccaacgag aaaggattcg ccccgtagat
    26881 ccttgtaaac tcgcaggcaa gttcccttta aatccgtatt gcgtaaaagc cccagctgcc
    26941 acctgtttat tgagatttaa tggcaggtca gcgtgtttaa taaataagtt tgccgcatgg
    27001 agtaagccgc tatcaatgtc gggcgacaac aacggatgct gaagacaact gctacattta
    27061 ccacaatgtt cggcacctgg ctcatcgagt gcgcggcgca gaaattgcat tttgcactcc
    27121 ttggtcgtga tatagctttc aacgtctgcc agctcacttt ttctctgatt catcaaatgg
    27181 ttaattcgtt catggtcagg agaaaaactg acaattgttc tgcgccataa tttctcggta
    27241 tacaccacag gcgatggatt ttcagctacc aataatttca gtgctttttc tatttgccca
    27301 taacggagat tcgtccgttg ttcaatgcct cgtaaggtaa gaccgtcatt cacgctaagt
    27361 acgttgagta tttcatgaat ttgcgcctcc gcaggaaagg cgctttcacg gaagaattta
    27421 tgaatagcac ggtcttcact gccgcaaagc agtatgccaa cagcagaatc gataccgcga
    27481 ccggcacgcc ccacctgttg gtagtaacca actatcgaac ctggcatctg gtaatgaata
    27541 acaaacccca aatccggttt gtcaaagccc attcccagcg ccgttgtcgc aaccagcact
    27601 ttaattttat ttgccagcaa cgcctgctcc agatattccc tggcagtatt agaatcttcc
    27661 gcgccttcac aagtcacgcc gctgtagtaa gcaaatgcac tgataccatt tttccttaac
    27721 cactcggcga caagttcggc atcgcgagta gtcagggtat aaacaattcc cgatttggaa
    27781 aactgaggga taaccgttgc taaccatgcc agacgggatg actgttcacc taagactaag
    27841 gcatcaagcg ccaaactttc gcgagccagc gttccacgct gaatcacaat gtcacccaat
    27901 tgctgacgga tatcctcaac gacacggtta ttcgctgtcg cggttgtacc cagaacaggg
    27961 gtattcgcag gtagttggcg caaaatatct aatatacgtc ggtaatccgg gcgaaaatcg
    28021 tggccccaat cagagatgca gtgcgcctcg tcgaccacaa gcaagccaat acgatcggct
    28081 ataggatata aaaccgtttc gataaagtcc tggtttgcca aacgttccgg ggaaaccagc
    28141 aagcaatcga cctcaccttg cagcaactta tcgctaatgc gctgccattc ttctctgttt
    28201 gtagaattca acgtttcagc cgtaatacct aaccgttcag cggcggcaac ctggttacgc
    28261 atcagggcaa gcaaaggaga gatgataatt gtcgggccag cgccgcgatc gcggaagatt
    28321 ttactggcga tgaagtacac agcacttttc ccccagccag tgcgttggac aaccagcagt
    28381 tttcgccgtt ggttcaccac cgcgtctatg gcttcccatt gcccttccct gaaagtagcg
    28441 gtggtatttc ctaacatccg ctgtaaaagc aattcagctg cgtgcttttc catctttatc
    28501 tcctgcgatc tttaggcagt gattagaacg gattatttgc aggtaaattt gcgagagaga
    28561 tcaaaaagat gaagagagga gaaaagagaa atgaattact gcgagcaagc tcgcagtaat
    28621 ttgtcgcagt aattaactca acagctggaa cgcaatcatc ccaacgatgg caccgacagt
    28681 gccgaggatg gtttccatca tggtccaggt tttcagcgtt tcggcttcgg tcgcgccggt
    28741 aaatttaccg aacaaccaga aaccagcgtc gttaacgtgg ctgacaacaa tcgaaccacc
    28801 ggcgatacaa atcgacagcg ccgccatttg cgcaccggag tagttcagtt gttcaataac
    28861 cggcatcacc agtcccaccg ccgttaaaca ggcaacggtt gcagaaccct gaatgatgcg
    28921 cactgcagcg gccagcacga agcaggtgat ggcaatcggc aggcccatgc cggttaacgc
    28981 ttcgcccagt gccggaccta cgccagagtc aaccagcacc tgtttgaaca cgccgcccgc
    29041 accaatcacc agcagaatga tccccgccgg ttgcagcgcg tgaccgcaaa tctccatcac
    29101 tttgtctttt ggcatgccct gacgcattgc caggccataa atcgctacca gacaagcaac
    29161 cagaatcgcg gtaaacggat gaccaataaa ctcgaaccat tcgtaagcgg tagagccttc
    29221 cggcacaaaa cgcgcggcaa tggttttcag ccccaccagc accagcggca gcaggatcag
    29281 cgacaggctg aatccgaagg acggcatttt gccttcgccg agatgcggct cgctgatgtc
    29341 gtcaggaata tgcagctcaa cgtaacggct gatgaagtta ccccacagcg gcccggcaat
    29401 aatcattccc ggaattgccg cacacaggcc aatcaggatc atccagccaa aatcggcgtt
    29461 catctgcgat gccagcagca ttggcgctgg ccctggcacc aggaacgccg ccgctgccgc
    29521 cacgcctgcg aataatggga ttaccagctt caccaggttc gtaccggtgt ggcgcgccat
    29581 tgagaaagca acgctaatca gcagaacaat cgccacttca aagaacagcg gtagcgcaca
    29641 gaccagcccc gcaaggccga tggcataatg cgcgcggctg tgaccgaagg atttaagcat
    29701 tttgacggca atctgatcga ctgcgccggt ttcatgtaag atcttgccaa acatagctcc
    29761 cagggcgaca accaccgcca ggaagccgag ggtgcctccc atcccttttt ccatcgtcgc
    29821 tgcgatttta tcgagcggca taccggaaaa aaggccagcc cccatggaca ccaccattaa
    29881 agccaggaaa gcgtgcatac gcgccttcat gactaaaaac agcagcagta aaacagaccc
    29941 tacagctgtt aaaacaagcg ttaatgtagt cactacttat ttgccttttt taataacctc
    30001 aatggtgctt gccacaacac cttccagcgg ttgatcgata tccaccacca gtacatcggt
    30061 ttcgtccgca cccggctcct gcagcgtttc aaactgcgtc accaacattt gggttttaaa
    30121 gaaatggcct ttgcgcgctt tcaggcggct ttcaatcaca tcaaaatcgc ctttcaggta
    30181 gatgaaagag agattcggat taccttcacg cagcaagtcg cgatagtgtt ttttcaatgc
    30241 agaacagacg atcagcgaca ctttattagt gcgctgcata gcaaacgcgg cgtcgttcag
    30301 cgcctgcaac cacggtttgc gatcgtcgtc attcagcggt tcgccagacg ccattttttc
    30361 gatattgcgc cgtggatgga ggaaatcgcc atcaagaaac gcggcatgaa gttgatgcgc
    30421 cacttcactt gcgaccgcag atttgccgct gcccgatacg cccatcaaga cgtaaatgtg
    30481 gtgatcatgg ttagtcgtgc tcaaagtggt gcccccacaa tacaagaatt aaaaatgtta
    30541 cgggtaactg ttatcggtaa cattctccgg ccggacaata tcagaagcaa tatccatccg
    30601 tgccttaagt gtataagtgt gagctacttc aaatttgtgg gcttaaatag atccgcccgg
    30661 tgacaaggtg aaacctaaat ctaacatttt cggtgtcaca gattcgccac gaatacgcgc
    30721 cagaaggcgt tcagcgccaa tactgcccat ccgctcacgc ggcgtcagca cgctcgcaag
    30781 tcgtggctcc atcacctgac caatgtcatg accgtggaaa ccggcaatcg ccatatcgtc
    30841 aggaactttt aaccccagac gctgacattc aaacgccgcg ccgaccgcca gatcatcatt
    30901 agtacagaac acgccatcca gctgcggata ttcccgccgc gcctggcgaa tcagttcaat
    30961 accggaagag taagaagaag attgctcaac catcacgcta tacggcacca ggcctgcatc
    31021 ccgcatcgcc tgttcgtatc ccttctgttt gatgatagta cgttcgtcga gacgtgcgcc
    31081 gagataggca atatggcgat gcccgcgagc aataatggcg gcggtcatct ggcgtgccgc
    31141 ttcaaagtta tcaaaaccga cggcaatatc gaggcatggc gacttgctgt ccatcagttc
    31201 caccacggga atccccgcca cttcaatcat ctttaaggtg cgcggcgtgt gggtacgttc
    31261 ggtgaggata agtccgtcga tattccatga gagcattgat tcgagacgtt cttgctccat
    31321 ttccggttta taaccgtagt gggccagcat ggtctgataa ccgtgcgcgt cggtgacgct
    31381 ttcgattccg cgtaatactt ccgcgaaaac ctggttggtg agagaaggta acaggacgcc
    31441 aatcgcccgg ctggtggcgt tagagaggat atcgggcgca cgattgggaa tatagcccag
    31501 ttcatcaaga gccgcggcaa tcttgccgcg tagagcgacg gaaacctgct ccgggttgcg
    31561 taaaaaacgg ctgaccgtca ttttggtcac gcctacacgg tcagccacat cctgaagtac
    31621 gggtcttttc tttttcatcg tcctgaaggt acaaaagaga tagattctct tagtttaaca
    31681 cggacgcatc agaaccttcc cggggaaaac agtgcttgtt tacaattatt agatatacac
    31741 taaatcaatt gcatcgcatt gtgctaacga cgcggcaaaa ccccggaagc gtacatcagc
    31801 acgcgaccgg agcgagcgag gaagcctgca aaaaggcagg cttcaaagat gccgttgagt
    31861 tttaaaccgg cggcagatcg aacagtaaca cttcgctgtc gctatccgca tggatggaga
    31921 ttgcctgctc atcccagatt gccagaccat cgctggtcga ggctttcacg ccgttaatgg
    31981 tgacattgcc tttcaccacc tggatccaga cgcggcgttc agcggcaatc tgatgcaccg
    32041 actgctcatc tttcagcaac gcccagcggt acagttccat atcctgatgc actttcaatg
    32101 aaccatcacg cgcatctggc gaaagcacca gttgtttgcc ctgcactgca tcgaagcgac
    32161 gctgttcata acgcggcgta atgccgtttt cttccggcat gatccagatc tgatacaggt
    32221 gcagacgctc ggtgctgctt gggttgtact ctgagtgacg aatacccgta cccgcactca
    32281 taatctggaa ctcgcccgcc ggaacctgct ctttattgcc catgctgtcc tggtgctcaa
    32341 cagtaccttc cagcacgtag gtcaaaattt ccatatcttt atgcgggtga gtgccgaagc
    32401 cctgccctgc ttcaatcacg tcgtcgttaa tcacgcgcag cgcggagaag cccataaagt
    32461 tcggatcgta atagttggca aaagagaaag tatgccagga gtccagccag ccatgatttg
    32521 catgaccacg ttcatttgct ttgcgtaagt agatcatttg tattcgcccc ctgaatgatt
    32581 tcgatgggct tagtgtggac ccgatctgcc tgggatgata gagggtgaaa attgacccct
    32641 ctgttcaaaa aaattgaact attcgagggg ccatcaaact tacttcgcga gagtcacggt
    32701 ggagggagag gcttgctcaa atccgcgttc aaggatttcc agattggtaa gaacttcaga
    32761 ttccttgacg taatttggcg caccgtgggt gatggtttga tacaacgcat cataaacgca
    32821 cccgtaatcg cccatctccg gcttcatctc ttctctgacc gtcacgccct cgtcattgac
    32881 atactccagc acaccgaccg aatcatccgc tgcgaatccc ggttcgcccg gcataatatt
    32941 agccttcagg ctggtttcct gctggtcgat accgtattta ataaacgaac ctttcttacc
    33001 gtgaacgata aatttcggat aatcgatttt caccagatgg ctggttttga cgatggcttt
    33061 tagatcgcca taaaacaact gcgcttcaaa ggtgtcgtcc gggttggctt tattacgcag
    33121 gctgcggatg tcataagcga cgtgatcagg gcgaccgaac agagaaataa tctggtccat
    33181 cgtatgcaca ccaaggccat agaacgcgcc atcctgcggc agtccaggtt tggtttctac
    33241 caccgggcgg taatagtcaa aatggctttc cacttcgaca atctctccca gcttgccgct
    33301 ttcaatcgct tttttcgccg tcaggaagca ggaatcaaag cgacgattct gatatggcgt
    33361 gacggtcagc cctttgcttt tcgccagtgc aaacagctct ttcgcctgcg caagtgtcgg
    33421 agtgaacggt ttttcgacca gcacattttt ccccgcttcc agcgcgcgct tcgcgtactc
    33481 gaagtggctg tccgcatggg tgcagacaac aaccagctta acatcgggat cgtttagcac
    33541 ttcgttgaga tcgctggtga aatggatatg agagtaaatg ggagcttgtt cttccggctt
    33601 tgcatggcgg cgaaaaatat gcgcgacatg ccagctatcc ttgcggttaa gtacatacgg
    33661 cagatggtaa cgggtggtgc ttttgccgaa gccaataaag gcgcagttga tgaccatgat
    33721 ttcgtccttt ttaaggtggt tatttacacc ttagcgcaaa gcggacgtgg ttcctactgg
    33781 agtgcgcata acgaacacaa gcacttcccg aggataaatt gagaacgaaa gatcaaaaaa
    33841 aaagccagca cccggctggc taagtaatac tggaagcaat gtgagcaatg tcgtgctttc
    33901 aggttctccg cgagggtctt cctgatcgcg agacaataat aatcattctc attcgcactt
    33961 gtccaacact ttttgcaaaa aaatgcattt gactcgcatt cgaaagtcaa tgatgttgaa
    34021 agggacattt accccaaaga ggacaaagga atgagtgaga tagtgatacg ccatgctgaa
    34081 acgcgcgatt acgaggccat caggcagatt cacgcccagc cggaggtgta ttacaacaca
    34141 ctacaggtgc ctcatccttc cgatcatatg tggcaggagc gactcgccga tcgtcccggc
    34201 atcaagcaac tcgtcgcctg tattgatgga gacgtcgtgg gccatctcac cattgacgtg
    34261 caacagcgcc cacgccgcag tcatgttgcc gattttggta tctgtgtcga ctcccgttgg
    34321 aagaatcgcg gcgtcgccag cgcccttatg cgagagatga ttgaaatgtg cgacaactgg
    34381 ttgcgggtag atcgcattga actaaccgtg tttgtcgata acgccccggc aattaaggtc
    34441 tataaaaaat acggctttga aattgaaggg actggtaaga agtacgcatt gcgtaatggt
    34501 gaatatgtcg atgcgtatta tatggcgcgg gtgaagtaaa gaatattctc aaattgcctg
    34561 ttaccaatgt gaataattag ttttgttcat ctaatttgag tt
//
LOCUS       DAZUCE010000020        34004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZUCE010000020 DAZUCE010000000
VERSION     DAZUCE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.020"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            235..1329
                     /gene="gcvT"
                     /locus_tag="ACHY03_000922"
     CDS             235..1329
                     /gene="gcvT"
                     /locus_tag="ACHY03_000922"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFS3904188.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1353..1742
                     /gene="gcvH"
                     /locus_tag="ACHY03_000923"
     CDS             1353..1742
                     /gene="gcvH"
                     /locus_tag="ACHY03_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFS3904189.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1861..4734
                     /gene="gcvP"
                     /locus_tag="ACHY03_000924"
     CDS             1861..4734
                     /gene="gcvP"
                     /locus_tag="ACHY03_000924"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFS3904190.1"
                     /translation="MTQTLSQLENRGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAVGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLADLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4898..6331)
                     /gene="bglA"
                     /locus_tag="ACHY03_000925"
     CDS             complement(4898..6331)
                     /gene="bglA"
                     /locus_tag="ACHY03_000925"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFS3904191.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            6376..6687
                     /gene="yqfB"
                     /locus_tag="ACHY03_000926"
     CDS             6376..6687
                     /gene="yqfB"
                     /locus_tag="ACHY03_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFS3904192.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            6851..7510
                     /gene="yqfA"
                     /locus_tag="ACHY03_000927"
     CDS             6851..7510
                     /gene="yqfA"
                     /locus_tag="ACHY03_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFS3904193.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGLVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(7587..8567)
                     /gene="ygfZ"
                     /locus_tag="ACHY03_000928"
     CDS             complement(7587..8567)
                     /gene="ygfZ"
                     /locus_tag="ACHY03_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFS3904194.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTVDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            8810..9076
                     /gene="sdhE"
                     /locus_tag="ACHY03_000929"
     CDS             8810..9076
                     /gene="sdhE"
                     /locus_tag="ACHY03_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFS3904195.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9057..9464
                     /gene="ygfX"
                     /locus_tag="ACHY03_000930"
     CDS             9057..9464
                     /gene="ygfX"
                     /locus_tag="ACHY03_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFS3904196.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9504..10025)
                     /gene="fldB"
                     /locus_tag="ACHY03_000931"
     CDS             complement(9504..10025)
                     /gene="fldB"
                     /locus_tag="ACHY03_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFS3904197.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10137..11033
                     /gene="xerD"
                     /locus_tag="ACHY03_000932"
     CDS             10137..11033
                     /gene="xerD"
                     /locus_tag="ACHY03_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFS3904198.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQCDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11058..11768
                     /gene="dsbC"
                     /locus_tag="ACHY03_000933"
     CDS             11058..11768
                     /gene="dsbC"
                     /locus_tag="ACHY03_000933"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFS3904199.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CSKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            11774..13507
                     /gene="recJ"
                     /locus_tag="ACHY03_000934"
     CDS             11774..13507
                     /gene="recJ"
                     /locus_tag="ACHY03_000934"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFS3904200.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLSWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSD
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERNIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13598..14696
                     /gene="prfB"
                     /locus_tag="ACHY03_000935"
     CDS             join(13598..13672,13674..14696)
                     /gene="prfB"
                     /locus_tag="ACHY03_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFS3904201.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14706..16223
                     /gene="lysS"
                     /locus_tag="ACHY03_000936"
     CDS             14706..16223
                     /gene="lysS"
                     /locus_tag="ACHY03_000936"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS3904202.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16266..16814)
                     /gene="idi"
                     /locus_tag="ACHY03_000937"
     CDS             complement(16266..16814)
                     /gene="idi"
                     /locus_tag="ACHY03_000937"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFS3904203.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVEHEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            16869..16940
                     /locus_tag="ACHY03_000938"
     CDS             16869..16940
                     /locus_tag="ACHY03_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904204.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(16937..17062)
                     /gene="yqfG"
                     /locus_tag="ACHY03_000939"
     CDS             complement(16937..17062)
                     /gene="yqfG"
                     /locus_tag="ACHY03_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFS3904205.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17064..18512)
                     /gene="uacT"
                     /locus_tag="ACHY03_000940"
     CDS             complement(17064..18512)
                     /gene="uacT"
                     /locus_tag="ACHY03_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFS3904206.1"
                     /translation="MSAIDSQLPSYSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18948..20867
                     /gene="ygfT"
                     /locus_tag="ACHY03_000941"
     CDS             18948..20867
                     /gene="ygfT"
                     /locus_tag="ACHY03_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFS3904207.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIPQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASA
                     DAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVTIGNLECYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20867..21355
                     /gene="ygfS"
                     /locus_tag="ACHY03_000942"
     CDS             20867..21355
                     /gene="ygfS"
                     /locus_tag="ACHY03_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFS3904208.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDCISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(21391..22758)
                     /gene="ghxQ"
                     /locus_tag="ACHY03_000943"
                     /gene_synonym="ygfQ"
     CDS             complement(21391..22758)
                     /gene="ghxQ"
                     /locus_tag="ACHY03_000943"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFS3904209.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22794..24110)
                     /gene="guaD"
                     /locus_tag="ACHY03_000944"
     CDS             complement(22794..24110)
                     /gene="guaD"
                     /locus_tag="ACHY03_000944"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFS3904210.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24128..25528)
                     /gene="xanQ"
                     /locus_tag="ACHY03_000945"
     CDS             complement(24128..25528)
                     /gene="xanQ"
                     /locus_tag="ACHY03_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFS3904211.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(25693..28563)
                     /gene="xdhD"
                     /locus_tag="ACHY03_000946"
     CDS             complement(25693..28563)
                     /gene="xdhD"
                     /locus_tag="ACHY03_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFS3904212.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28560..29339)
                     /gene="ygfM"
                     /locus_tag="ACHY03_000947"
     CDS             complement(28560..29339)
                     /gene="ygfM"
                     /locus_tag="ACHY03_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFS3904213.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLAVDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDIEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29390..30718)
                     /gene="ssnA"
                     /locus_tag="ACHY03_000948"
     CDS             complement(29390..30718)
                     /gene="ssnA"
                     /locus_tag="ACHY03_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFS3904214.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNPPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30721..33819)
                     /gene="ygfK"
                     /locus_tag="ACHY03_000949"
     CDS             complement(30721..33819)
                     /gene="ygfK"
                     /locus_tag="ACHY03_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFS3904215.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLSRHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLFVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSKGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         8320 a         8629 c         8999 g         8056 t
ORIGIN      
        1 gtatagaaac tggtgtattt tttggttttt tattctgtcg cgatttttgc attttttaac
       61 cataagctaa tgtgatgatc aattttacct tatggttaac agtctgtttc ggtggtaagt
      121 tcaggcaaaa gagaacgatt gcgttgggga ccgggagtgg ctccgatgct gggttttgtg
      181 gtgataattt caccatgaaa aagttgtcag ccccgcttat tcaatgagga caagatggca
      241 caacagactc ctttgtacga acaacacacg ctttgcggcg ctcgcatggt ggatttccac
      301 ggctggatga tgccgctgca ttacggttcg caaatcgacg aacatcatgc ggtacgtacc
      361 gatgccggaa tgtttgatgt gtcacatatg accatcgtcg atcttcgcgg cagccgcacc
      421 cgggagtttc tgcgttatct gctggcgaac gatgtggcga agctcaccaa aagcggcaaa
      481 gccctttact cggggatgtt gaatgcctct ggcggtgtga tagatgatct catcgtctac
      541 tactttactg aagatttctt ccgcctcgtt gttaactccg ccacccgcga aaaagacctc
      601 tcctggatta cccaacacgc tgaacctttc ggcatcgaaa ttaccgttcg tgatgacctt
      661 tccatgattg ctgtacaagg gccgaatgcg caggcaaaag ctgccacact gtttaatgac
      721 gcccagcgtc aggcagtgga agggatgaaa ccgttctttg gtgtgcaggc gggcgatctg
      781 tttattgcca ctaccggtta caccggtgaa gcgggctacg aaattgcgct gcccaatgaa
      841 aaagcggcgg atttctggcg cgcgctggtg gaagcgggcg ttaagccatg cggcctgggc
      901 gcgcgtgaca cgctgcgtct ggaagctggc atgaatttgt atagtcagga gatggacgaa
      961 accatctctc ctttagccgc caacatgggc tggaccatcg cctgggaacc ggcagatcgt
     1021 gactttatcg gtcgtgaagc tctggaagcg cagcgtgaac atggcacaga aaaactggtt
     1081 ggtctggtga tgaccgaaaa aggcgtgctg cgtaatgaac tgccggtacg ttttaccgat
     1141 gcgcagggca accagcatga aggcattatc accagcggga ctttctcccc gacgctgggt
     1201 tacagcattg cgctggcgcg cgtgccggaa ggtattggtg aaacagcgat tgtgcaaatt
     1261 cgcaaccgtg aaatgccggt taaagtgacg aaacctgttt ttgtgcgtaa cggcaaagcc
     1321 gtcgcgtgat ttactttttt ggagattgat tgatgagcaa cgtaccagca gaactgaaat
     1381 acagcaaaga acacgaatgg ctgcgtaaag aagccgacgg cacttatacc gttggcatca
     1441 ccgaacacgc tcaggagttg ttgggcgata tggtgtttgt tgacctgccg gaagtgggcg
     1501 cgacggttag cgcgggcgat gactgcgcgg ttgctgaatc agtaaaagcg gcgtcagaca
     1561 tttatgcgcc agtaagcggt gaaatcgtgg cggtaaacga cgcactgagc gattccccgg
     1621 aactggtgaa cagcgaaccg tatgcaggtg gctggatctt taaaatcaaa gccagcgatg
     1681 aaagcgaact ggaatcactg ctggatgcga ccgcatacga agcattgtta gaagacgagt
     1741 aacggcttta ttcctcttct gcgggagagg atcagggtga ggaaaattta tgcctcaccc
     1801 tcactctctt cgcaaggaga gaggttcaca attcactgca cgtttcagga accatcgctc
     1861 atgacacaga cgttaagcca gcttgaaaac cgcggcgctt ttattgaacg ccatatcgga
     1921 ccggacgccg cgcaacagca agaaatgctg aatgccgttg gcgcgcaatc gttaaacgcg
     1981 ctgaccggcc agattgtacc gaaagatatt cagcttgcga ccccgccgca ggttggcgca
     2041 ccggcgaccg aatacgccgc gctggcggaa ctcaaggcta ttgccagtcg caataaacgc
     2101 ttcacgtctt acatcggcat gggttacacc gccgtgcagt taccgccggt tatcctgcgt
     2161 aacatgctgg aaaatccggg ctggtatacc gcgtatactc cgtatcaacc tgaagtctcg
     2221 cagggccgcc ttgaggcact gctcaatttc cagcaggtaa cgctggattt aaccgggctg
     2281 gatatggcct ctgcttctct tcttgacgaa gccaccgctg ccgccgaagc aatggcgatg
     2341 gcgaaacgcg tcagcaaact gaaaaatgcc aaccgcttct tcgtggcttc cgatgtgcat
     2401 ccgcaaacgc tggatgtggt ccgtactcgt gccgaaacct ttggttttga agtgattgtc
     2461 gatgatgcgc aaaaagtgct cgaccatcag gacgtcttcg gcgtgctgtt acagcaggtt
     2521 ggcactaccg gtgaaattca cgactacact gcgcttatca gcgaactgaa atcacgcaaa
     2581 attgtggtca gcgttgccgc tgatattatg gcgctggtgc tgttaactgc gccgggtaaa
     2641 cagggcgcgg atattgtttt tggttcggcg caacgctttg gcgtaccgat gggttacggt
     2701 ggcccccacg cggcattctt tgcggcgaaa gacgaataca aacgctcaat gccaggccgt
     2761 attatcggtg tatcgaagga tgcagctggc aataccgcgc tgcgcatggc gatgcagact
     2821 cgcgagcaac atattcgtcg tgagaaagcg aactccaaca tttgtacttc ccaggtgctg
     2881 ctggcaaaca ttgccagtct gtatgccgtt taccacggtc cggttggcct gaaacgtatc
     2941 gcaaaccgca ttcaccgtct gaccgatatc ctggcagtgg gcctgcaaca aaaaggtctg
     3001 aaactgcgcc atgcgcacta tttcgacact ttgtgtgtgg aagtagccga caaagcgggc
     3061 gtactggcgc gtgccgaagc ggctgaaatc aacctgcgta gcgatattct gaacgcggtg
     3121 gggatcaccc ttgatgaaac caccacgcgt gaaaacgtga tgcagctttt cagcgtgctg
     3181 ctgggggata atcacgggct ggacatcgac acgctggaca aagacgtggc tcacgacagc
     3241 cgctctatcc agcctgcgat gctgcgcgac gacgaaatcc tcacccatcc ggtgtttaat
     3301 cgctaccaca gcgaaaccga aatgatgcgc tatatgcact cgctggagcg taaagatctg
     3361 gcgctgaatc aggcgatgat cccgctgggt tcctgcacca tgaaattgaa cgccgccgcc
     3421 gagatgatcc caatcacctg gccggaattt gccgaactgc atccgttctg cccgccggag
     3481 caggccgaag gttatcagca gatgattgcg cagctggctg actggctggt gaaactgacc
     3541 ggttacgacg ccgtttgtat gcagccgaac tctggcgcac agggcgaata cgcgggcctg
     3601 ctggcgattc gtcattatca tgaaagccgc aacgaagggc atcgcgatat ctgcctgatc
     3661 ccggcttctg cgcacggcac taaccctgct tctgcacata tggcaggaat gcaggtggtg
     3721 gttgtggcat gtgataaaaa cggcaacatc gatctggctg atctgcgcgc gaaagcggaa
     3781 caggcgggcg ataacctctc ctgtatcatg gtgacctatc cttccaccca cggcgtgtat
     3841 gaagaaacga tccgtgaagt gtgtgaagtc gtgcatcagt tcggcggtca ggtttacctt
     3901 gatggcgcga acatgaacgc ccaggttggc atcacctcgc cgggctttat tggtgcggac
     3961 gtttcacacc ttaacctaca taaaactttc tgcattccgc acggcggtgg tgggccgggt
     4021 atgggaccga tcggtgtgaa agcgcatctg gctccgtttg taccgggtca tagcgtggtg
     4081 caaatcgaag gcatgttaac ccgtcagggc gcggtttctg cggcaccgtt cggtagcgcc
     4141 tctatcctgc caatcagctg gatgtacatc cgcatgatgg gcgcagaagg gctgaaaaaa
     4201 gcaagccagg tggcaatcct caacgccaac tatattgcca gccgcctgca ggatgccttc
     4261 ccggtgctgt ataccggtcg cgacggtcgc gtggcgcacg aatgtattct cgatattcgc
     4321 ccgctgaaag aagaaaccgg catcagcgag ctggatattg ccaagcgcct gatcgactac
     4381 ggtttccacg cgccgacgat gtcgttcccg gtggcgggta cgctgatggt tgaaccgact
     4441 gaatctgaaa gcaaagtgga actggatcgc tttatcgacg cgatgctggc tatccgcgca
     4501 gaaattgatc aggtgaaagc cggtgtctgg ccgctggaag ataacccgct ggtgaacgcg
     4561 ccgcacattc agagcgaact ggtcgccgag tgggcgcatc cgtacagccg tgaagttgcg
     4621 gtattcccgg caggcgtggc agacaaatac tggccgacag tgaaacgtct ggatgatgtt
     4681 tacggcgacc gtaacctgtt ctgctcctgc gtaccgatta gcgaatacca gtaattcact
     4741 gattcgacta tcttctaaag gcgcttcggc gcctttttag cttgttaata aagtgcgctt
     4801 tgtgtgtcgg atgaagcgta aatataaaat cctggagttc aatatatgac aggagcgcca
     4861 ggcctgatga gcggggcgca tcaggcacat cgggtaatta aagcttctcg ccgttgctgg
     4921 caatcacctc tttgtaccag ttaaagctct tcttacgtga acgcgacata tcgccagtac
     4981 cgtcgtcatg tttattcaca tagataaagc cgtagcgttt gctgtactgc ccggtggtga
     5041 acgacacgca gtcgatacag ccccacggcg tgtagcccat caggtctacg ccatcgtaag
     5101 tcaccgcctt tttcatctct tcgatatggg cgcgtaggta gtcaatgcgg tagtcgtcgt
     5161 tgatgctgcc atcttcttcc actttgtcgt aagcgccaaa accgttttcg acaataaaca
     5221 gcggcttctg gtaacgctca tacagttcgc aaagcgcgta acgcagaccc acagggtcaa
     5281 tctgccagcc ccagtcagac gctttaacat acgggttcgg tacgctgcct tcaaaaccag
     5341 atatcgcatc gccggtgccg ccttcggcct tcactgcgtt ggtcatgtaa tagctgaaac
     5401 caagataatc gcaggtgcct tcgcgcagca catccagatc gccgtcttcc attttgatgt
     5461 taaatccgcg acgctcccac tcgttcaaca cataggacgg gtaatagccg cgtagctgca
     5521 catcggtaaa gacgtagcgt tcgcgcatcg actcctgagc gaacatcaca tcgtccgggt
     5581 tacaggagta aggatagagc ggcaccatcg ccagcataca gccgactttc atctccgggt
     5641 taatgcgacg cgcagctttc accgccaggg cgctggcgac aaactggtga tgcagcacct
     5701 gatacatcgt ctcttccggg ttctcatgct cggtataaac cacgccggag cagcagtaac
     5761 cgaacagcgg tgcacgccag ttacgctggt tgttaatttc gttgaaggtc atccagtatt
     5821 tgactttatg cttatagcgt tcaaatacca cttccgcgaa acggacaaag aaatcaacca
     5881 ctttacggtt ggtccagcta ccgtattgct gcaccagatg cagcggcatt tcaaagtggg
     5941 agagggtgat cactggttcg atgttgtatt tcagcaattc atcgaacaga gagtcgtaaa
     6001 acttcagccc ttcttcgttt ggctgagctt catcgccttt tggaaaaatg cgcgtccagg
     6061 cgatggatgt acgaaaacat ttgaagccca tttcggcaaa tagcttgatg tcttccttgt
     6121 agtgaccata aaaatcaacg gcttcatggt ttggatagta tttgcctggc aagacttctt
     6181 tggtgatttc gcgcggcacg ccgtgtgcgc caccggtcag aacgtcacaa atgctcggcc
     6241 ctttgccgcc tttgttccag ccgccttcga cctgatgagc ggcaactgcg ccgccccata
     6301 agaaatcttt cggtaaggtg agttttttca ctatcatagg ctccagatga gtcaattctt
     6361 acaggaaata gtaccatgca gccaaacgac atcacttttt ttcaacgttt ccaggatgac
     6421 attctggctg ggcgtaaaac catcaccatc cgcgacgagt ctgaatcgca cttcaaaacg
     6481 ggtgatgtgc ttcgtgtcgg gcgttttgaa gatgacggtt atttttgcac gattgaagtc
     6541 accgcaacct caaccgtaac gctggatacg ctgacggaaa aacatgcaga gcaggaaaat
     6601 atgaccctga ctgaactgaa aaaggtcatt gccgacatct atcccggtca gacacagttt
     6661 tatgtgattg aatttaaatg tctttaaatt gaactcacgc ctgttagttg aaatttcgca
     6721 tttaatttca tgatttttag gggattagtc tcaaatcaga atttatttta gctaacaggt
     6781 gttcactgga actattctca gttacgctgg agaggtatac atgtgcgtgt acgtttctcc
     6841 ggagtaagtt atggttcaga agcccctcat taagcaggga tattcgctgg cagaggaaat
     6901 tgccaacagc gtcagtcacg gcattgggtt ggtgtttggt atcgttgggc tggtgttgct
     6961 actggttcag gcggtggatc tcaatgccag cgccacagcg ataaccagct acagcctcta
     7021 tggcggcagt atgatcctgc tgttcctcgc ttcgacgctc tatcacgcca ttcctcatca
     7081 acgggcaaaa atgtggctga agaaatttga ccattgcgct atttacctgt tgattgccgg
     7141 aacctacacg ccgtttttgc tggtggggct ggattctccg ttagcgcgcg ggttgatgat
     7201 tgttatctgg agcctggcat tgctggggat tctgttcaaa ctgaccatcg cgcaccgatt
     7261 caaaatttta tctctggtga cttatctggc gatgggctgg ctatcgctgg tggtaattta
     7321 tgaaatggca gttaagctcg cggcgggcag cgttacctta ctggcggtag gcggcctggt
     7381 ttattcgctc ggggtgattt tctacgtctg caaacgcatt ccatacaacc atgccatctg
     7441 gcacggcttc gtgctcggcg gtagtgtgtg ccactttctg gcgatctatt tgtatattgg
     7501 gcaggcgtaa tgggtatgcc tttgtaggcc tgataagacg cgccagcgtc gcatcaggcg
     7561 ataaatgcct gatgcgacac agagatttac tcttcgagcg aatacggcag cggctcgata
     7621 tgcaatgtat tcgcatcgtc acgcacgcgg aagatgctgt ccggttccat atcgttattc
     7681 atgacgacct gtaccacgac ctgaccatct tccagtttta ccgcagccag caccgtaccg
     7741 gtgcgacgcc agttctcgcc cattttcagc tctaaatctt caccagcttc cggcagtcgg
     7801 ctggcactac ctgtcagcaa ccagagcgcg cgtttgttgg caccacggaa ttttgctcgc
     7861 gccaccatct cttgtccggt atagcagcct ttcttaaagc tgataccgcc cagcgcctgg
     7921 agattggtcg cctgtggaat aaactgcccg ctgttggcgg catcaatcac cgggaaaccc
     7981 gcttcaatgt ttaatgccag ccactgttgg ctattgttca gttccgcttc accgcgcagt
     8041 ttatcggtca gcatattggc agtagcttca tcggttacga tcaggaaacg ttctgccggg
     8101 tgttcaaacc atagcaaagt ggtcgcgcct tctttaacta cctgtttttc tttcgaaggc
     8161 agttcactaa agagattggc cagtgcggcg cgcgcctgaa aaccggcaac accaagcagc
     8221 acacgctcgt cgtctggcgc gatggtcact ttagagaata ccgcatattt tttcagttca
     8281 gtcagctgcg gttcacgcac gctgcgccgt tcaatccacg caaagccatc gccgtcgcgg
     8341 aacagacgta aattgctcca cattttacct ttggcgtcgc aatgggcggc cagcaggtgc
     8401 tgatctactg tcatctggct gacgtctgct gtcacctgac cctgcatata tttttcgctg
     8461 tccgcgccag taatggtggc aagcgcccag tcatcaagcg tcatcagtgt cagtggcaaa
     8521 cgggcagaag ccgtaggctg acggggagga aaaggtgtaa aagccataat agagtcctga
     8581 gttgcttaac ggcgtattaa taactaatgg taaaagagct atgaggcaat gcaagtgctt
     8641 tcactacgcg atctgtgcct tctgcgaggc gacttccaaa aaaatgaagc ccttgaattg
     8701 ttgtgaatta atttcgcgat cgcgattgtg tttactgata ttcccgtaaa agaagtcgaa
     8761 aaacacgtta caatagcggg gtactggatt ttcgtgagac aggaagaaga tggacattaa
     8821 caacaaagcc cgcattcatt gggcatgccg ccgtggtatg cgcgaactcg atatttcaat
     8881 catgccgttt ttcgaacatg agtacgacag cttaagcgat gacgaaaaac gcatctttat
     8941 tcgtctgctg gaatgtgacg atccagacct gttcaactgg ctgatgaatc acggtaaacc
     9001 ggccgatgca gaactggaaa tgatggtccg actcatccag acacggaacc gggaacgtgg
     9061 tcctgtggca atctgatttg cgcgtctcct ggcgcgcgca gtggctttcc ttgctgattc
     9121 atgggctggt tgccgctgtt attttactca tgccctggcc gctcagttac accccgttat
     9181 ggatggtgtt actttcgctg gtggtgtttg attgcgttcg cagccagcgg cgcattaatg
     9241 ctcgccaggg ggaaattcgc ctgttgatgg acgggcgttt gcgttggcaa gggcaggagt
     9301 ggagcatcgt caaagcgccg tggatgatta agagcggcat gatgctgcgt ttacgttctg
     9361 atggcggtaa acggcaacat ttatggctgg cagccgacag catggacgaa gccgaatggc
     9421 gggatttacg gcggattttg ttgcaacaag agacgcaaag ataaagatga cggcaaagca
     9481 tatgcgctaa gccgtcagtg agatcaggcg taatgctctg ccatttcgtt gaggatttgc
     9541 tcgcaccagc tctgaatacg ctcgtcgcta aggtcatact ggttagtttc atccagcgcc
     9601 agacccacga acagttgccc gtcagcaatc accggtttcg ggctggtaaa ttcatatcct
     9661 tccgttggcc agtagccgac gaactttacg cctttggtcg agagtttgtc atgcagcata
     9721 ccgagcgcat cgaggaacca ctcgccgtat cccagttgat cgccaagccc atacagcgca
     9781 acaattttac cttcgaggtt caggtcgtcg agctgatccc agacggcttc ccagtcttcc
     9841 tggatttcac caaaatccca ggtcgggata cccagaatga gcacatcgta ctgctccatt
     9901 aatttcgggg agtcgtcctt gaggttatgt aaggtcacca gttctgggcc gataatatcg
     9961 cggatttttt ctgccgccat ttcggtgtaa caggtgctgg aaccgtaaaa aagacccata
    10021 ttcataacgt aagcttctcg attctgctgt ggcttttgcg gctagtgtat cagaatcgct
    10081 ttagtcacgg cacaataagg cataatggat caaatgagtg accataaggg gcgcaagtga
    10141 aacaggatct ggcacgcatc gagcagtttc ttgatgctct gtggctggaa aaaaatctgg
    10201 ctgaaaatac gttgaacgct taccgtcgcg atctgtcaat gatggtggag tggttgcatc
    10261 accgcgggtt gacgctggcg acggcgcaat gtgacgattt gcaggcatta ctggcagaac
    10321 ggctggaggg cgggtataaa gccaccagct cagcgcgttt gctgagtgca gtgcgccgat
    10381 tgttccagta tctttatcgc gaaaagtttc gtgaagacga tcccagtgcg catctcgctt
    10441 caccgaaatt gccccagcgt ttgccaaaag atttaagcga agcgcaggtt gaacgtttat
    10501 tacaggctcc attaattgat cagccactgg agctacgcga taaagccatg cttgaagtgt
    10561 tgtatgctac cgggctgcgt gtctctgaac tggtcggact gacaatgagt gatatcagcc
    10621 tgcgtcaggg cgtagtgcgg gtcattggta aaggcaacaa agagcgcctg gtgccgttag
    10681 gtgaagaggc ggtttactgg ctggaaacct atctggaaca tgggcgtccg tggctgctga
    10741 atggtgtgtc aattgacgtg ttgtttccca gccagcgtgc gcagcagatg acgcgacaga
    10801 ccttctggca ccgtattaaa cattatgccg tgctggcggg tatcgacagc gaaaagctgt
    10861 caccgcatgt gttgcgtcac gcttttgcca ctcatttatt aaatcatggt gcggatttac
    10921 gcgtggtgca gatgctactg ggccacagcg atctttccac cacgcaaatt tatacgcatg
    10981 tcgctaccga gcgtctgcgg caacttcatc aacagcatca cccgcgggcg tgatgtctga
    11041 aaagaacggg aagatttatg aagaaaggtt ttatgttgtt tactttgtta gcggcgtttt
    11101 caggctttgc tcaggctgat gacgcggcaa ttcaacaaac gttagccaaa atgggcatca
    11161 aaagcagcga tattcagccc gcgcccgtag ctggcatgaa gacagttctg actaacagcg
    11221 gtgtgttgta catcaccgat gatggtaaac atatcattca ggggccaatg tatgacgtta
    11281 gcggcacggc tccggtcaat gtcaccaata agatgctgtt aaagcagttg aatgcgcttg
    11341 aaaaagagat gatcgtttat aaagcgccgc aggaaaaaca cgtcatcacc gtgtttactg
    11401 atattacctg tggttactgc cacaaactgc atgagcaaat ggcagactat aacgcgctgg
    11461 ggatcaccgt gcgttatctt gccttcccgc gccaggggct ggacagcgat gcagagaaag
    11521 aaatgaaagc tatctggtgt tcgaaagata aaaacaaagc gtttgatgat gtgatggcag
    11581 gtaaaagcgt cgcaccagcc agctgcgacg tggatattgc cgaccattac gcacttggcg
    11641 tccagcttgg cgttagtggt actccggcag ttgtgctgag caatggcaca cttgttccgg
    11701 gttaccagcc gccgaaagag atgaaagaat tcctcgacga acaccaaaaa atgaccagcg
    11761 gtaaataatt cgcgtgaaac aacagataca acttcgtcgc cgtgaagtcg atgaaacggc
    11821 agacttgccc gctgaattgc ctcccttgct gcgccgttta tacgccagcc ggggagtacg
    11881 cagtgcgcaa gaactggaac gcagtgttaa aggtatgctg tcctggcagc aactgagcgg
    11941 tgtcgaaaag gccgttgaga tcctttacaa cgcctttcgc gaaggaacgc ggattattgt
    12001 ggtcggtgat ttcgacgccg acggtgccac cagcacggct ctaagcgtgc tggcgatgcg
    12061 ctcgcttggt tgcagcgata tcgactacct ggtaccaaac cgtttcgaag acggttacgg
    12121 cttaagcccg gaagtggtcg atcaggccca tgcccgtggc gcgcagttaa ttgtcacggt
    12181 ggataacggt atttcctccc atgcgggggt tgagcacgct cgctcgttgg gcatcccggt
    12241 tattgttacc gatcaccatt tgccaggcga cacattaccc gcggcggaag cgatcattaa
    12301 ccctaacttg cgcgactgta atttcccgtc gaaatcactg gcaggcgtgg gtgtggcgtt
    12361 ttatctgatg ctggcgctgc gcaccttttt gcgcgatcag ggctggtttg atgagcgtaa
    12421 catcgcaatt cctaacctgg cagaactgct ggatctggtc gcgctgggga cagtggcgga
    12481 cgtcgtgccg ctggacgcta ataatcgcat tctgacctgg caggggatga gtcgcatccg
    12541 agccggaaag tgccgtccag ggattaaagc gctgcttgaa gtggcaaacc gtgatgcaca
    12601 aaaactcgcc gccagcgatt taggttttgc gctggggcca cgtctcaatg ctgccggacg
    12661 actggacgat atgtccgtcg gtgtggcgct cttgctgtgc gacaacatcg gcgaagcgcg
    12721 cgtgctggca aatgaactcg atgcgctaaa ccagacgcga aaagagatcg aacaaggaat
    12781 gcaggttgaa gccctgaccc tgtgcgagaa actggagcgc agccgtgaca cgctacccgg
    12841 cgggctggca atgtatcacc ccgaatggca tcagggggtt gtcggtattc tggcttcccg
    12901 tattaaagag cgttttcacc gtccggttat cgcctttgcg cccgcgggtg atggtacgct
    12961 gaaaggttct ggtcgctcca ttcaggggct gcatatgcgt gatgcgctgg agcgattaga
    13021 cacactctac cctggcatga tgctgaagtt tggcggtcat gcgatggcgg cgggtttgtc
    13081 gctggaagag gataaattcg aactctttca acaacggttt ggcgaactgg ttactgagtg
    13141 gctggaccct tcgctattgc aaggcgaagt ggtgtcagac ggtccgttaa gtccagccga
    13201 aatgaccatg gaagtggcgc agctgctgcg cgatgctggc ccgtgggggc agatgttccc
    13261 ggagccgctg tttgatggtc atttccgtct gctgcaacag cggctggtgg gtgaacgtca
    13321 tttgaaagtc atggtcgaac cggtcggcgg cggtccgctg ctggatggta ttgcttttaa
    13381 tgtcgatacc gccctctggc cggataacgg cgtgcgcgaa gtgcaactgg cttacaagct
    13441 cgatatcaac gagtttcgcg gcaaccgcag cctgcaaatt atcatcgaca atatctggcc
    13501 aatttagcgt catcttctct ataaaaaaga gcgtggattg ggtacaatcc cgctcttatc
    13561 accgcatttt gactagctca ataaaagaaa tcagaccatg tttgaaatta atccggtaaa
    13621 taatcgcatt caggacctca cggaacgctc cgacgttctt agggggtatc tttgactacg
    13681 acgccaagaa agagcgtctg gaagaagtaa acgccgagct ggaacagccg gatgtctgga
    13741 acgaacccga acgcgcacag gcgctgggta aagagcgttc ctccctcgaa gccgttgtcg
    13801 acaccctcga ccaaatgaaa caggggctgg aagatgtttc tggtctgctg gaactggctg
    13861 tagaagctga cgacgaagaa acctttaacg aagccgttgc tgaactcgac gccctggaag
    13921 aaaaactggc gcagcttgag ttccgccgta tgttctctgg cgaatatgac agcgccgact
    13981 gctacctcga tattcaggcg gggtctggcg gtacggaagc acaggactgg gcgagcatgc
    14041 ttgagcgtat gtatctgcgc tgggcagaat cgcgtggttt caaaactgaa atcatcgaag
    14101 agtcggaagg tgaagtggcg ggtattaaat ccgtgacgat caaaatctcc ggcgattacg
    14161 cttacggctg gctgcgtaca gaaactggtg ttcaccgcct ggtgcgtaag agcccgtttg
    14221 actccggcgg tcgtcgccat acgtcgttca gttccgcgtt tgtttacccg gaagtcgatg
    14281 acgatatcga tatcgaaatc aacccggcgg atctgcgcat tgacgtttat cgcgcgtccg
    14341 gcgcgggcgg tcagcacgtt aaccgtaccg aatctgcggt gcgtattact cacatcccaa
    14401 ccgggatcgt gacccagtgc cagaacgacc gttcccagca caagaacaaa gaccaggcca
    14461 tgaagcagat gaaagcgaag ctttatgaac tggagatgca gaagaaaaat gccgagaaac
    14521 aggcgatgga agataacaaa tccgacatcg gctggggcag ccagattcgt tcttatgtcc
    14581 ttgatgactc ccgcattaaa gatctgcgca ccggggtaga aacccgcaac acgcaggccg
    14641 tgctggacgg cagcctggat caatttatcg aagcaagttt gaaagcaggg ttatgaggaa
    14701 ccaacatgtc tgaacaacac gcacagggcg ctgacgcggt agtcgatctt aacaatgaac
    14761 tgaaaacgcg tcgtgagaag ctggcgaacc tgcgcgagca ggggattgcc ttcccgaacg
    14821 atttccgtcg cgatcatacc tctgaccaat tgcacgcaga attcgacggt aaagagaacg
    14881 aagaactgga agcgctgaac atcgaagtcg ccgttgctgg ccgcatgatg acccgtcgta
    14941 ttatgggtaa agcgtctttc gttaccctgc aggacgttgg cggtcgcatt cagctgtacg
    15001 ttgcccgtga cgatctgccg gaaggcgttt acaacgagca gttcaaaaaa tgggacctcg
    15061 gcgacatcct cggcgcgaaa ggtaaactgt tcaaaaccaa aaccggcgaa ctgtctatcc
    15121 actgtaccga gctgcgtctg ctgaccaaag cactgcgtcc gctgcctgat aaattccacg
    15181 gcttgcagga tcaggaagcg cgctatcgtc agcgttattt ggatctcatc tctaacgatg
    15241 aatcccgtaa cacctttaaa gtgcgctcgc agatcctctc tggtattcgc cagttcatgg
    15301 tgaaccgcgg ctttatggaa gttgaaacgc cgatgatgca ggtgatccca ggcggtgccg
    15361 ctgcgcgtcc gtttatcacc caccataacg cgctggatct cgacatgtac ctgcgtatcg
    15421 cgccggaact gtacctcaag cgtctggtgg tcggtggctt cgagcgtgta ttcgaaatca
    15481 accgtaactt ccgtaacgaa ggtatctccg tacgtcataa cccagagttc accatgatgg
    15541 aactctacat ggcttacgca gattacaaag atctgatcga gctgaccgaa tcgctgttcc
    15601 gtactctggc acaggatatt ctcggtaaga cggaagtgac ctacggcgac gtgacgctgg
    15661 acttcggtaa accgttcgaa aaactgacca tgcgagaagc gatcaagaaa tatcgcccgg
    15721 aaaccgacat ggcggatctg gataacttcg actctgcgaa agcgattgct gaatctatcg
    15781 gtatccacgt tgagaagagc tggggtctgg gccgtatcgt taccgagatc ttcgaagaag
    15841 tggcagaagc acatctgatt cagccgacct tcattactga atatccggca gaagtttctc
    15901 cgctggcgcg tcgtaacgac attaacccgg aaatcacaga ccgctttgag ttcttcattg
    15961 gtggtcgtga aatcggtaac ggctttagcg agctgaatga cgcggaagat caggcgcagc
    16021 gcttcctgga tcaggttgcc gcgaaagatg caggtgacga cgaagcgatg ttctacgatg
    16081 aagattacgt taccgcactg gaacatggct taccgccgac agcaggtcta gggattggca
    16141 tcgaccgtat ggtaatgctg ttcaccaaca gccataccat ccgcgacgtt attctgttcc
    16201 cggcgatgcg tccggtgaaa taagcattac gttatgctca caaccccggc aaatgtcggg
    16261 gttttttatt taagctgggt aaatgcagat aatcgtattc tggcttcgcg atttgtcgcc
    16321 tgcatcacca tccacggact gaacgcccac ggcgtggcat caataccgcg taatacatca
    16381 gctaaatcac accattgata atccatcact tcatcatcgt tgatctgtaa cgcactggtg
    16441 gtgcgtgcgg caaataccgg acacacttca tgttccacaa tgccattcgg atcggtggcg
    16501 cgatagcgaa agtcaggata gatagattca ggaggcgtaa tttccacgcc aagctcataa
    16561 cggcaacggc ggatcaccgc gtcttcgttg ctttctccta gttgtgggtg cccacaaacc
    16621 gagttagtcc acacgccagg ccatgctttt ttgctcagag cgcgacgggt aactaataat
    16681 tgtcctttgg cattaaacag ccaactggag aacgcgagat gtaagcgggt gtctgccgtg
    16741 tgtgcggcat acttttccag cgtacccgtg ggaactccct gtgcattcaa taaaatgacg
    16801 tgttccgttt gcataatttc tcacatgtaa ttctgatcac tcatcattat agataattga
    16861 agagtgaaat gattaaccaa gtgagcgttt atcgacaacc gcccgttttg agcggatgtc
    16921 gacaggtaaa aaccatttaa aaaagtagca tcatatattt gaagcgactt tcaatggcca
    16981 ttgcaacaca gtcagaaatg acaggaatag agagagtaaa aagcaacaac agactgaata
    17041 tagcgcgcat taaaagattc atattattct ccatgctcat ttttcttcag cttgcgcatc
    17101 agaagccaca tacgtactgt cctgactttt aaatctttat cagagacgga ttccttcacc
    17161 aggtcagcat gatgctgata gccattaaag aagacgttca gcacaacggc gctaagggtt
    17221 gcgagcataa tgccgctatg tagcagcggt tgcagtacgg ccggtaactt agaaaagaaa
    17281 tcgtgagaga gcgtcggagt catgccaacg ccgagactga tcgccacaat atagaggttg
    17341 taacggttgg tggtgtagtt acagcgcgac agaattcgaa tccctgtcgc cagtaccatg
    17401 ccgaacatca ccagcccagc gccgcccagc acaaattgcg gaatggaggc taccagcacc
    17461 gccatttttg gcaccatgcc aaacaggatt aatataattc ccgaagaaat acacacccag
    17521 cggctatgaa cgcgcgtcac gctaaccagg ccaacgtttt gagaaaatga cgtgtggggg
    17581 aagctgttaa acgtgccacc aatcattgtc cctacgccat cgacacgcag cccgcgaata
    17641 atatcgtgcg aagagagttt acgaccgact atttcaccca gtgccaggaa catccccatc
    17701 gactcgataa acacgatgat taacacggca gtcatggtca gaatggaaac gggatcgaaa
    17761 atcggcatac caaacgacat cggcgtaaca atcgcaaacc acgaggcatc atgtagcccg
    17821 gataaattga cttcattcat catccacgaa agtaaaaagc caaatacaat ccccagtaat
    17881 acggcgacgt tggacataaa tcctttcgca tagcgagtaa tgagcaagat aaaaattaag
    17941 acggcaaagg agatacctaa ataaacggga ttaccatatt gcggattccc tttacctccg
    18001 gcggcccagt caatacccac ctgaatgatg ctaagcccga tagaagtaat aaccacaccg
    18061 gtaaccagtg gcgggaataa aggcatcaag cgaccgataa gtggcgctaa taatgtggtg
    18121 ataaaacccg cggcgatagt ggcaccaaat atacccagca ggccgatatc cgggttcatc
    18181 ccaatggcta tcattggtgt tacagcagca aaggtcaccg acataatcac cggcaggcgg
    18241 atccccataa agcggccgat acctatacat tgcaataatg tgacgatccc gcagcaaaag
    18301 agatccgagc taatgagcat cgcaatagct tcttttgaga ggcccagtcg gtcaccaatc
    18361 ataagaggaa cagcgactgc acctgcgtac atgacaagga cgtgttgcag accgagtatg
    18421 atcagctttc ctggtgataa tatgcggtca acctcatcag ttgggcggtc ttgcccagaa
    18481 tatgagggaa gttgggaatc tatggcgctc atcagtgttt ctcctttcgc ccgccagagt
    18541 aaaaagcaac cgtttacgtt aatgaaactg tctaaacaca tgctcgtgga gtgccagttt
    18601 ttgcgaggaa ggggagttag agacaggaat tgcggaggca atcacataat cgtgcttaaa
    18661 ccacgaaaat gagaagagaa cctgacaatg gaattatctg taattttctc atattgatat
    18721 ttcattaatc attctggcat gtcgcactct cgcatttaat cgtttttatc tggatagtgc
    18781 tcttttgatc ggcaatgttg aaataatgaa agcggcatag attgaggggc aataatttga
    18841 actcagtcgc aaaattataa ttaaaacggt catgctacct aatatatgcc cgtttattgc
    18901 atcgcgaaat ttgagaagac ataactcatt aaatgaaagg gatgcaaatg aataagttta
    18961 tcgctgctga agctgcggaa tgtataggct gtcatgcttg tgaaattgcc tgtgcggtgg
    19021 cacacaatca agaaaactgg ccgctgagtc acagtgactt tcgaccacgt atccacgttg
    19081 tagggaaagg cctggctgcg aatccggtgg cctgccatca ctgcaacaat gccccttgcg
    19141 ttacggcttg tccggttaat gctctgactt tccagtccga tagcgtacaa ctggacgagc
    19201 aaaaatgtat tggttgtaaa agatgcgcaa tcgcttgccc ctttggcgtc gttgagatgg
    19261 tcgatacgat tccgcagaaa tgcgaccttt gtaaccagcg cagttccggc acgcaagcct
    19321 gtattgaagt ttgcccaaca caggcgttac gactgatgga cgataaaggg ttacagcaga
    19381 taaaggtggc ccgccagcgc aaaacggcag caggaaaagc gtcagcagac gctcagccat
    19441 ctcgcagtgc agcgttgctc cccgttaact cgcgtaaagg cgcagataaa atttcagcaa
    19501 gtgaacggaa aaaccacttt ggcgaaatct attgcgggct ggatccacaa caagcgactt
    19561 atgagagtga ccgctgtgtt tattgtgccg aaaaagctaa ctgcaactgg cattgcccgc
    19621 tgcataacgc tattccggat tacatccgtc tggtacagga gggaaagatt attgaagcgg
    19681 cagaactttg ccaccagacc agttccttac cagaaatctg cggcagggta tgcccacagg
    19741 accgtctttg tgaaggcgca tgtactttga aagatcactc tggcgcagtc actatcggta
    19801 atctggaatg ctacatcacc gataccgcgc tggcgatggg ctggcgtccc gatgtcagca
    19861 aagttgttcc ccgtatcgag aaagtggcgg tgattggcgc tgggcctgca gggttagggt
    19921 gtgctgatat tctggcgcgc gcaggagttc aggtcgatgt ctttgatcgc catccagaaa
    19981 ttggcggtat gctgactttt ggcattcctc ctttcaaact cgataaaacg gtattaagcc
    20041 agcggcgaga gatattcacc gcaatgggaa tcgactttca tcttaactgt gaaattggcc
    20101 gcgatattac ctttagcgat ttaacttctg aatatgatgc agttttcatc ggcgtgggga
    20161 cttacgggat gatgcgagca gatctgccgc atgaagatgc gcccggtgtc attcaggctc
    20221 taccgttctt gactgcccat acccgccagc tcatggggtt gccggagtct gaagagtatc
    20281 cgctgacgga cgtggaaggt aagcgagtcg tggtattggg cggtggcgat acgacaatgg
    20341 attgtttgcg gacttccatc cgcctcaatg ccgccagcgt gacctgcgcg tatcgtcgtg
    20401 atgaagtcag tatgccgggc tcgcgcaaag aggtggtcaa tgcgcgcgag gaaggtgtcg
    20461 agtttcaatt caatgttcag ccgcaatata tcgcttgtga cgaagatggg cgtttaactg
    20521 cggtgggcct gattcgtacc gccatgggtg agccggggcc ggatggtcgc cgtcgtcctc
    20581 gtccggttgc gggttcagag tttgaattgc ccgccgatgt tctcattatg gcctttggtt
    20641 tccaggcgca tgccatgccg tggttgcagg gcagcggaat taaactcgat aaatggggcc
    20701 tgattcaaac cggcgacgtt gggtatttac ctacccagac gcatctgaaa aaagtctttg
    20761 ctggtgggga tgcagttcat ggcgcggatc tggttgtcac tgccatggcc gcaggaaggc
    20821 aggcggcgcg cgatatgtta actctgtttg atacgaaggc atcgtgatga aatcgttaat
    20881 tatcgttaat ccggctgact gtattggctg ccgtacttgt gaagtggcct gtgtggtcgc
    20941 tcacccttca gaacaggagt tgaatgccga tatcttttta ccccggctga aggtacagcg
    21001 actggactgc atcagtgcgc cggtgatgtg ccaccaatgt gaaaacgccc cttgtgttgg
    21061 cgcttgcccc gtgggggcgc tgacgatggg cgagcaggtg gtgcagacca attctgctcg
    21121 ttgtattggc tgtcagagtt gcgtcagtgc gtgcccgttt ggaatgatca ccattcagtc
    21181 gttgccgggg gatacccggc aacaaatcgt gaaatgcgat ctctgtgaac agcgggaaga
    21241 ggggcctgcc tgcgttgaat cttgcccaac acaggcgctg cagttgctta ctgaaagaga
    21301 actcaggcga gtccgccagc agcgtattgt tgccagcggt gagaatcctc tgtgaaagaa
    21361 atctccgcgc tgaaaggcgc ggagaatcta ttagattgcc caaccacccg cgtaaaatgc
    21421 gaccagtgcg gcagtaatga tcaccgtacc aatattcagc ttttgccatt cgcgtgcaaa
    21481 gacgcggcct acgaccagtg tcacaaagcc cagcataata ccggtaacga tattacaagt
    21541 cagaacgatg aacacggcac acaccaggcc agccatagcg tcaataaaat cattgaaatc
    21601 cagcttcgag acgttactta acatcagcaa acctacgtac atcagagcgg gtgcagtggc
    21661 gtaaccaggg atcaaaaatg acagcggtga taagaacaga atcagcagga ataacgcccc
    21721 cactacggtt gcggttaacc ctgttttacc cccggcggcg gttcctgccg ctgattcgat
    21781 ataaaccgct gcgggcgctg cgcccaccag gccggagaat attgaactta ctgagtcact
    21841 ggtcagggct ttgccgccgt tgatgatctg gttgtcttta tccaacaaat tcgcctgacc
    21901 ggcgacggca cggatggtgc cagtagcgtc gaacactgcg gtcatcacca atgccagtac
    21961 actcggaagt acagttggct ggagtgcgcc cataatatcg aggctgaaaa tcagagactt
    22021 accatcttcg ccagtcaggc ttggcatcgc caccagaccg tggtatttca ccgctgggtc
    22081 aaagattaag ccgatgatcg aaattgcaat aatcaccaac aagatcccgc cgggtacgcg
    22141 acacttctcc agaccgaaga tgaccgccag ccccagcaag ctcatcatca ccgggaagga
    22201 ggtaaacgca ccgagcgcca ctggcaagcc ttcaatcggg tttttgataa ccatacccac
    22261 accgttagca gcaatcagca gcagaaacag cccgataccg atacctgtac cgtgagcgat
    22321 ccccatcggt aaattacgta agatccaggt acgcacaccg gtaacggaaa tggcggtgaa
    22381 gatgacgccc atcagaaata ccgcgcccag tgcgacagga acgctaattt gttgcccgag
    22441 taccagactg aatgcggtaa acgccgtcaa ggaaatcgcg caaccaatcg ccattggcaa
    22501 attggcccat aatcccatca gcaacgagcc gaagcccgcg accagacagg tggcaacaaa
    22561 cacagctgcg ggaggaaaac ctgctttgcc cagcattccc ggaacgacga taacggaata
    22621 aaccatggcc agaaaggttg ttaatccagc cagtacttcc tgacgaacgg tactgccacg
    22681 agcggtaatt ttaaaataat tatccagcgc gccctgtggc tttggtgcgt ccggtgtttg
    22741 taggatgtct ccagacataa tgatgtcctc acgaggatgt aaaagtttta ttattagttg
    22801 cgttcgtaca ccagacgacc atcaacgtag gtgcggtaga tcgaacggtc atcgcccaac
    22861 gtcatcatca cgaacaattt gtcgactaaa gaaacagagt tgtcatagcg cagctgctgt
    22921 agcggagtgg cggtgggttc catcaccacg aaatcagcct ctttgccagg taaaaagttg
    22981 ccaatcaaat cgtcaaggcc cagagatttc gctccgccga gcgtggccag gtaaaacgct
    23041 tcatatgccg agaggcgata gccttgtaat tgcaacactt tgtaggcttc gttcagcgtt
    23101 tgcagcatgt tgaaagttgt tccggcaccg atatccgttc ccatgcccac tttgactttc
    23161 ttctgccatg cttttttcaa gttgaataag ccgctgccga ggtaaaggtt ggaggtcgga
    23221 cagaaagcaa tgctggattt ggtttcgctg agacgatccc actctttttc ttcgagatgg
    23281 acgcagtgag caaagacaca gtttttaccg gtcaggccgt actgatggta aacatccaga
    23341 taaccatcat ggtcaggata aagcgatttc acccaggcaa tttcatcttt gttttcacag
    23401 agatgggtat gtacccacgt atccggatat tcctctttca ggcgttgcgc catcgccatc
    23461 tgttcaggag aagaagtagg ggcgaagcgt ggtgtaatcg catatagcag acgaccattt
    23521 ttgtgccagc gttcaatcag ttctttgctt tggtgatagc tgctttcggc agtgtcgagc
    23581 agataatccg gtgcgttacg gtccatcatc accttaccgg caatcatacg catattgata
    23641 tgactggcgg cttcaaacag cgcatcaaca gattgcggat gaacagtgcc aaacaccagc
    23701 gccgtggtgg ttccgttacg taaaagctgc ttgatgaaga acgccgacat ttcgcgggcg
    23761 tactctaaat cctcataacg acgttcagta gggaaggtgt gtttattcaa ccactccagc
    23821 aattgctcac cataggcccc caccatttca ctttgcggat aatggatatg tgtatcgaca
    23881 aagcccggta ctatcagttt gccgcgatag tcgcgcacgc gaatggtgtc aggaatttga
    23941 tgctttccgt tttcccattc gccaaaccat tccacttttc cctgtttaat gagtaataaa
    24001 ccatcctcaa taaaccgcag cgcagaggca atctcttccg ggttatcgac cgtacgggtg
    24061 acatcaataa aactgcctcg taccgctttt aacgtgtgtt ctcctgacat cattgactcc
    24121 tttactgtta atccatctct tccgctgagg taataccagg cagaacgttt tcctgtcggt
    24181 agccaccagg gaggataata tttaataaaa tcgcagttaa cccgccagca caaatagggt
    24241 tttcaactaa tacataaata gaggctggca atattttaaa aatttcggga tcgtaggaga
    24301 cgccaagccc taaacctaaa gaagtggcga caataagtgt ttcacgccgc tttaatccgt
    24361 tggtgatgat gatgcgaatc ccagcgatgg caatcatgga aaacatcaac gtcattgcgc
    24421 ctcccagaac tgccgaggga atggtcgtga agaagccgcc aatcatcgga aataagccga
    24481 ggataaccag cattaccgcg atggttcgcc cgacataacg tgaagcgacg ccagtcatct
    24541 gaataacccc attattttgc gcaaacgtgg ttaatggtaa tgaaccaaca gcggaggcga
    24601 taacagaaac cagaccatct gccagcacgc cgcctttcag tcgggactga tactcttccc
    24661 cctgaatggg gcggcgggaa accattgccg tggcggtgat atcgccgact gcttccagca
    24721 cgctaagcag ataaatcgtg ccaaccacca ggaactgatg gaagctaaaa ctaaagccgt
    24781 atttgaacgg atgcgggatg gtgattaacg gcagattgcg catactgctg aaatccacca
    24841 tgcccaggca taacgatgcg atatagccga cacatagccc aatggcgatc cctcccatgc
    24901 gtagcaacgg actgcgacag cagttaaagc cgatcaccac aatcaaaacc aataaaccaa
    24961 cgccgagatg ttcgtaattg ccgaacgtac cgctgctttt ggctgcaaat cctccaccaa
    25021 aatcgataat gccgacttta atcaggctta agccgatcat cagtaccaca ataccgctga
    25081 cggtaggcgt aataacccga cgtaaatagg gcaggataaa agaagagccg acaaccagaa
    25141 atgcgccaac gaaggagacg ccaagcagcg acgacatgat taactcttcg tgaaaaccgt
    25201 cgcttttcat gctgctgccc agcgcaatca tgaccgtaac aaatgaaaaa ttgactgact
    25261 gaattgagag taggccagaa ccgacgatgc cgtagcggtt tacttgtaac caggtaccaa
    25321 taccagaggc gatcatcgcc atagaaacaa gataggcagt tgtttcagcg gaaagctgta
    25381 aggccgcacc cacgattaac gcgggggtta ccatcggaac gaaaattgcc aacagatggg
    25441 taatggcacc aacgagagcc tgatgaaagg gagggcgatc ctccagttca aatataaggt
    25501 cagaacctgc atggtttata tcagacatcc cttcactcct tttaaagtaa atttgatcat
    25561 ctgaattagt aaaataaatt cccacgaatt tggggcgcag gacgccttga gaacggctta
    25621 tctcattttc gcagtcacta tttagtcatc gttcaaacag agaaacaggc cgccgaagcg
    25681 gccatttatt acttatattt tttccagcgc ggtgagtatt ttctccggtg tgaaatgcca
    25741 ttcgcgtaac cagatgccgc aggcatcgtg aattgcggta gcaatcgccg gagctgcgcc
    25801 atttacacca atttccgaga tcgatttcgc cccgaacggg ccgactttat cgtcgctcgg
    25861 caccaacaca gcgcggaaat cacgcggaat gtcaccaatt ttcggtgcgc cgtaactgcg
    25921 taaatcacgc gttaacgggt gaccttcggc gtcataaatg atctcttcgc tcatactgtg
    25981 gccgatagca cgcagggtgg caccgtagat ttgtcccaaa gctaactctg gattgaccgg
    26041 tgtaccgcag tccagcaagg cgtagaattt atccaggcgg atttcacccg tacgcgtgtt
    26101 gacggcaact tcagcgaagt ttgcgccata cgggaaagcg aaatcaggcg tgatataact
    26161 gccagttccc accagtgaac caaagccggt gccggtttcg cctttatggg caatatcccc
    26221 gaaactaact tcgcctttct tgccgcgcac gacgcccggc gttgctagtt gaacatctgc
    26281 cactggctca cccaacattt gcgcgccgtg gaacagaatt ttctcccgca gattttccgc
    26341 tgccaaacgc gccgcgttac ccgagaagca agtaccggac gaggcatatg cgcctttatc
    26401 aaacaacgca tgatcagtat caccggagat aacatgcacg tcctgcggtg ggcagtgcag
    26461 cacttctgct gccagtttcg tcactacagt atccagacca gtaccaatat ccgcaccgcc
    26521 agaatgaacg ataaaggtgc cgtccgattc cagtttgatc atgcagttag cctgatcgat
    26581 atccgggata cccgatttct gcatgataat ggcgacgccg cgaccgatgt gccagtcacc
    26641 attttgcggt ttcggtgaag accattggat catctcgcga ccctgacgca ggatctcttc
    26701 cagtgcgcag ctggcggcgg aaggaacgga ggtcggcgct ttaccttcac cgattgcacc
    26761 gagaattttc agctcttgcc cttcgtgtac ccggttacgt tcgataattt ccagttggtc
    26821 gatctgtaac tgttcagcca gttccgctaa tgccatggtg atagcgaagt tacctttcgg
    26881 tgcgccataa ccctgataag caccatttgg gcaaatgttg ctgtagtagg tggtgacctg
    26941 gaaatcgacg ttatcgcacg gatataacgg cagcgacagc gccggtccgt tacatggtac
    27001 ggtgagtgag tggttgccgt aagggccagt gttggcgcgg aaatccatct tcactgccgt
    27061 caggcgacca tcttttttcg ctcccagttt gacggtgact ttcgcgacgt gacgagaggt
    27121 gttagcaata aactcttctt cacgggtgta gcggaacagg accggacgcc cggtcacgca
    27181 ggttgcccag gcgcacactt cttccagcag gatgtcctgt ttggaaccaa aaccgccgcc
    27241 aactcgttct ttaatgacat gaactttatg ctgtttcatg cccacgaggc gcgcgacctg
    27301 gcggcgtaag tgccatggta cctgggtgga ggcgtggata accagacgat cgccgtccat
    27361 acgagtaaag cagatatgtg tttcagtcgg gcactgctgc gcttgcgttg agttataggt
    27421 tcgctcaatg atcacatcgg catcggcaaa gcctttgtcc atatcgccaa tatgaccatg
    27481 aatactggcg gcgatatttt tgcgggggcg agaaccgatc gggaagttaa tgatcatatg
    27541 ctcgccgcgc tgggctgcat ggctgttatc gtcttccaga gtatctggcg caccagcaac
    27601 atacaccacc ggttcatcgt gcacgacagg cgcatcttcc gccattgctt cgtcgatcga
    27661 cattaccggc ttaagcactt catattcaac gtcgataagc ttcagtgctt cgagcgcaat
    27721 ttcttcactt tctgcgacca ccgcagcaac acgatcgccg acgtgacgca ttttcttgcc
    27781 gaacatacgg cggtcaagcg gtgacggttc cggtgcgctc tgcccccccg gggtatagta
    27841 gatatccggg caattcagat gagtaataac gtgaacgacg cccggtaagg cttcagcttt
    27901 gctgacatcc agatgagtaa tcagtgcgtg agcgtgtggg ctacgtaaca ttttaatgac
    27961 gcaggcgtca gccgttacgc ggtcttcaac atagcagggc ttcgcctgca ccatttttgc
    28021 ggcatcagtt ttaggataat gcttaccaat gacttcaagg tcgtcacgga aagtcggagc
    28081 gatatcaatg gtggcctgcg gattattttt acgtgcaacc gccagttcaa tgacctgata
    28141 gtattgctgc cagccagcat cgcggctaaa cagacctgag agcgcgtcgt cgatctcttc
    28201 gcgggtaggt gcggcgatgc gatcgagaag atcggtgata atcagagccg cagctggatc
    28261 gttataacca gactgcacca cgccaacatc aaccattgcc tgttgaacca gacttaactc
    28321 gttccatttg cccagagatt ctgcggtacg aatatctgcc ttctctaact gtgcggcaat
    28381 aagcaaggac gcgttaacga tattaccgtt aaagattatt gcgtcagaac cggcaaaccc
    28441 gaagccatca tcactgttgc gtacagagtg cattcccatg ttaaacaaca gcttttgcac
    28501 gttttcgcct ggattaacgg ttagctcctg aggcgcgcca tttaaagtaa agtggatgat
    28561 catacggctt cctccccagc ctgttggcag tcggcataca ggtcggctac cagaactccc
    28621 gtgatatagc gtttataggc cacgctgccc cgcaaatctt cctgcgggaa aatggcgttg
    28681 gcgacagcct gttcaagtgc attgccttcc agattttgtt tttcgatatc gtgcagacgc
    28741 agtgctttac tggcgacgcc atccagcgca attcgcatac cgtcgtggtc tgtcattgca
    28801 acggctgccg tcacgacggt taaacctgcc tgagaacggc taattttgcg ggtcgcacag
    28861 gtgcgatacg gatctttaat gataatttcg gttagcaggc gatcgcatgg gcaggccagg
    28921 tagtcctcga ttgacagcgt ttcgccgttg ccaaaaacca gttcagcatc cactgccagc
    28981 aggacgggaa gcagcaccga ctcttcctgc cgggcggcga tttcaccacc aatggtcgac
    29041 tgattacgaa catggcgtga gtaaacaaaa ccgagggctt cacgcagcgc tgcaggaata
    29101 aatcgcgcat cacgcagtgg ctgcaagcga gacattgcgc caatccgcag tgcaccgtta
    29161 tcccagtcaa cccagtccag ttccagatcc tgtaaggaga tggcaatctt tttatcggta
    29221 cgggttggtg tagcattgag tttgctgccc ccggcgaacc agacggcttc atcctggtag
    29281 cggcgcttca gttccagcgc ctgttcgaca gagtcgggcc tgaaaaattg ttcaatcatc
    29341 ttctttcctt atttaaccct cttccctgcg ggaagagggc atctgtcatt tatgccagcg
    29401 catccatccg acgccacata ctggcagcgg cttttctggc ttgtgcataa atggaatcgc
    29461 aatcgaagtt aaactgacgg tcttcataga ccatcacacc attgaccatc acgctgtgaa
    29521 cgctgcctga gcccataccg aaagcgatat gcccggcgat attgtctgcc agcagcggcg
    29581 tcggcgggtt gtaatcgcaa atggttaaat cagctttgta accggcttcc agaagcccaa
    29641 atttcgcgcc aaagttgcgg ctcatcagtt cgttaccgtt agtcagggct ttggcaaaac
    29701 tgtcaggcca cagcggacca cccgcatcgc gatgtttaaa gaaggcaaat ttcatctctt
    29761 caaacatgtc cgaaccaatg ccgtccgttc ccaacgccag attgcggatg tcgctaaggt
    29821 gatggttgta gccgacatgg ttgttcatgt ttgaacgggc gttatgcacc aggaacgcat
    29881 cgcgctgatt gagtagggtg atgtcatctt tcgacaagta cagcccatga gcgaccagcg
    29941 ttttgctgtc gatgagatcg aattgcgcca gtcgtgccag caggtctttg ccgtaccagt
    30001 ggtgactgta ggaaacgtcg taaaggtctt ccgcagcgtg aatatgcaaa ccacggcctg
    30061 tggctttcac ggcttcacgc agcatctcca gaccggcatc tggcacggta aacggcgcgt
    30121 gagcaccgat atgtgcttcc accagatacg gctcggttgc tgctttcttc gcctcatcaa
    30181 tctgacgggc aaagcggatg ttttcttcta caccttcctg caactctttg atgccgttgt
    30241 tacggtcagt agtttcaaaa caggtcatcg cgcgcaggcc aacttttaaa aatgcgtcgc
    30301 gcaatgtgga gagcgacccg ccgatatacg ccggagaggc atggtgatcg ataaccgatg
    30361 tacatccgct cttaatcgct tccagggaac aaatcagtcc gctgtaatag agcgactctt
    30421 catcaagggc gcgatcgagc cgccaccaga gatttttcag cgttgagatg aaatccgggc
    30481 agggggcgat gtttgccata attccgcggg aaagccccga gtaaaaatgg ttgtgcgagc
    30541 agacaattcc cggcatcaca atccgaccat gcatctcttt gtagctggca tcggggtagc
    30601 gttgcgtcag ggcatctccg atagcgacaa tcacatcatt ttcgatagcg atatcaacgc
    30661 cttcctgcac tttcgccggg tgtaactgca ctgcggtgac attcttcaga atcaacatga
    30721 ttacacctcc acgcggccca gcagataatg atgatgctgg tggacatggc tgatgatgcg
    30781 gcacatatcg ttcagctccg gtggtacgtt gttaaactga cctttgctgt cgatgtttaa
    30841 cacccagctt tggttattca gacgtactcg tacccggcaa tcttccacaa ggaagcctgg
    30901 gttgctgctg ttatcaaagt cttgcgccag gctgaagacg gtgattttgt ctttgtacgg
    30961 tttaccgttc cacggacaga actgagcgca gttgccgcat tcgttacagt aagcgtcgag
    31021 gtgcagcgtc tggaaacggt tctggaagcc tgggaccgca atggagacgt tggcgcggtt
    31081 cgggcagaca tccacacact tgctgcaaac gtagttacat tcgaggcagc gagcggcttc
    31141 ctgggcgaca aacgcgtcac gatcgtcact gttcaccagc gtgatagaga tatcgccttt
    31201 acgttgatag atttccgccg gattgacgtt gttccagtat ttatcgttct ggtgggaacg
    31261 gatattttcc cgactaagga tggcatcggt cgcccgacgc gcggttccga cagcagcgac
    31321 aatggaggat ggtccgcgct gtacgtcgcc gatcataaag acgtcagtca gacgagtttc
    31381 gccattatgg tcgacgtctg gccagccgtt tttgtccagc ggcacgccca tcgcattcag
    31441 ggcttcagta tcctgctgtt caccaatggc ggtgatcagg ctgtctacaa acagtgttac
    31501 tgtttcgttg gtttcaaccg gacgacgacg acctttctca tccggttcgc caagcgacat
    31561 aacgcgcaag gttaaggtgc catcagcatc gaaacgttcc ggattgttca ggaaacggaa
    31621 ctccacgccg tcgtgcaacg cttcttcata ctcttcgcgc catgcgggca tctcttgcag
    31681 tgaacgacgg taaacgatcg ttgctttttc tacgcctgga acgcgtaacg ccgcacgagc
    31741 gcagtccatt gcggtgttac ccgccccgac aacgaccaca tgtttgccca gcttgagcgc
    31801 tgtacccttg ttgtattcac gcaggaaggg gagtgatttc cagacatttt ggttgtcgcc
    31861 cgccagtttc acaccgctat ttttatcagt gccggtggca atcagaacat agtggaagcc
    31921 ctgatttttt aactgttcaa cggttaaatc gggtgagcag ccatactcaa atttcacgcc
    31981 gtgatcggca acaaaatcga tatcgtgctg aattaactct gcaggaatac ggaactgagg
    32041 gatgatattt ttcaccacgc cgcctgcatt ggcttcgcgt tcaaacagcg taaccggatg
    32101 gcccgctctg gcaaggaagt aacctgctgc cagacccgcc ggacctgcac caatcacggc
    32161 aaccggatgg cgtgaaccag aaccggctgg tttgtgccaa cgttgcttat attcatccca
    32221 acctttttcc agcgcgactt ttttcagttc gcggatattc agcgcactgt cgtaatccag
    32281 gcgggtacag ttgtattggc actggtgatc gcaaatatga ccggtaatgg caggtagggc
    32341 gttgcgttgg tagatgagtt ccagcgcgtc ggcatagcgg tgttcgccaa gcagacggat
    32401 gtattccgga atatcttgct tgatagcgca ggcagtaaca cagggggcaa cgtagcagtc
    32461 ggtcagcggc aggtcttctg ccacttcaat acgctcttct ggcttccagt gtttctgggt
    32521 gtattccatg gttaacgcgt ctgctgccag tctgttcagt cgttcgacgt caacatggtc
    32581 aagtccccag gcatcggagc cttccagctc gcgcatgcag gcacttaagc gcagatagcc
    32641 accaggtttc agcaggtcgg ttgccatcgt aatagggcga ataccagtat caaaaatatc
    32701 gcggatagtc agctgactgg caccaccgga ataagaaatg ggcagtttgc cgtcaaaggc
    32761 gcgagagaga actgctgcaa cattgatgga gagcgggaac agcgcacggc ctgacatata
    32821 catctcttca ccgggcagtg cgcctttatt gttgatggtg ccgagagtgt tagtcagttt
    32881 tacgccaaag ccgagtgatt tttcttttgc cagtgccatc aggcgttcca gcatttccag
    32941 tgcttgcgtc agcttgaggt cgtgatcaaa tgactcttct tttaagccta tgtaaccgaa
    33001 accgcaaaca tcgaggatct cacgaacacg cgcgtacccg agcaaggtcg ggttaagttt
    33061 gacaaaggtg ttgagccctt tttcttccag catgtagcgg caaatggctt cgatttcatg
    33121 cggaggacag ccgtgcatgg tggagagggt gacgccctgc accatactgg tagggatgcg
    33181 agcgggtaaa gcttgcaagc tttcgcgttt ttcctgcaat ccgtggcggg ataaaaatgc
    33241 gtcatcctgg agtaatttat tcagcgtatc gcgatattgc gcgaatttcg gatggtcaga
    33301 tgcatccatc atattgtcga tgaactgttg catcggcggt tgcttaatac cttcgaggtt
    33361 ataaccgacg ctcatattaa agatgaacga tttaccggaa tcagaaggct ggaacatcgc
    33421 ttcgagaagg tgcagggcaa accaggcttt gaggtattca tcccaggctt taagcagggt
    33481 aaattcggta gaccattcgg tgttaaagca ctcgtcttcg gcatcgatac aaggcttttc
    33541 cagctccagg cggtcaagaa tttggacggt ttttagttcg atgaagcgtc cgccagtcag
    33601 ccaggaagtg acaatatttt gcgcgagctg cgtgtgcggg ccagcggcag ggccgacggg
    33661 agtggcacag gtttcaccga agacgctaac agttttacct tttacgggtg agtaaaactg
    33721 ttgctcggga ataccaaaga ttgagcgttg ttgttggtat tcatcaaata tgcgcgtcaa
    33781 aagttcctca aacggaatgg gacgcataat atcccccata accctctcct tagcttatac
    33841 agatatcatt gagtgaccgg tgttgagaaa tccggtggtt tagggggata gagcaacaat
    33901 cgcaccactc ttgccaggtt tggcgggaaa tataaaaaaa tgtgaggcgg ctcgaaactt
    33961 aagggctttt tgtgaatgaa ctcacaggtg ccaaatgaga atta
//
LOCUS       DAZUCE010000021        33635 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZUCE010000021 DAZUCE010000000
VERSION     DAZUCE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.021"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            15..251
                     /gene="ydhI"
                     /locus_tag="ACHY03_000950"
     CDS             15..251
                     /gene="ydhI"
                     /locus_tag="ACHY03_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFS3904216.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            254..1111
                     /gene="ydhJ"
                     /locus_tag="ACHY03_000951"
     CDS             254..1111
                     /gene="ydhJ"
                     /locus_tag="ACHY03_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFS3904217.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLSKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSNSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1111..3123
                     /gene="ydhK"
                     /locus_tag="ACHY03_000952"
     CDS             1111..3123
                     /gene="ydhK"
                     /locus_tag="ACHY03_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS3904218.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMAA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3124..3645)
                     /gene="sodC"
                     /locus_tag="ACHY03_000953"
     CDS             complement(3124..3645)
                     /gene="sodC"
                     /locus_tag="ACHY03_000953"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFS3904219.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3726..4622)
                     /gene="ydhF"
                     /locus_tag="ACHY03_000954"
     CDS             complement(3726..4622)
                     /gene="ydhF"
                     /locus_tag="ACHY03_000954"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFS3904220.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4671..4910)
                     /gene="ydhL"
                     /locus_tag="ACHY03_000955"
     CDS             complement(4671..4910)
                     /gene="ydhL"
                     /locus_tag="ACHY03_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFS3904221.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5013..5612
                     /gene="nemR"
                     /locus_tag="ACHY03_000956"
     CDS             5013..5612
                     /gene="nemR"
                     /locus_tag="ACHY03_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFS3904222.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            5649..6746
                     /gene="nemA"
                     /locus_tag="ACHY03_000957"
     CDS             5649..6746
                     /gene="nemA"
                     /locus_tag="ACHY03_000957"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFS3904223.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6827..7234
                     /gene="gloA"
                     /locus_tag="ACHY03_000958"
     CDS             6827..7234
                     /gene="gloA"
                     /locus_tag="ACHY03_000958"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFS3904224.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7337..7984
                     /gene="rnt"
                     /locus_tag="ACHY03_000959"
     CDS             7337..7984
                     /gene="rnt"
                     /locus_tag="ACHY03_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFS3904225.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8077..12693
                     /gene="lhr"
                     /locus_tag="ACHY03_000960"
     CDS             8077..12693
                     /gene="lhr"
                     /locus_tag="ACHY03_000960"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFS3904226.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTSEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILSARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTISNIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPAMISGQERLACMDDAARLRDALGVQLPESLPAIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPTHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(12744..13091)
                     /gene="grxD"
                     /locus_tag="ACHY03_000961"
     CDS             complement(12744..13091)
                     /gene="grxD"
                     /locus_tag="ACHY03_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFS3904227.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13426..14253
                     /gene="mepH"
                     /locus_tag="ACHY03_000962"
     CDS             13426..14253
                     /gene="mepH"
                     /locus_tag="ACHY03_000962"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFS3904228.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHTVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14381..14962
                     /gene="sodB"
                     /locus_tag="ACHY03_000963"
     CDS             14381..14962
                     /gene="sodB"
                     /locus_tag="ACHY03_000963"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFS3904229.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15108..16277)
                     /gene="ydhP"
                     /locus_tag="ACHY03_000964"
     CDS             complement(15108..16277)
                     /gene="ydhP"
                     /locus_tag="ACHY03_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3904230.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSIAHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLVIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16443..16532)
                     /gene="cydH"
                     /locus_tag="ACHY03_000965"
                     /gene_synonym="ynhF"
     CDS             complement(16443..16532)
                     /gene="cydH"
                     /locus_tag="ACHY03_000965"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFS3904231.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16831..17856
                     /gene="purR"
                     /locus_tag="ACHY03_000966"
     CDS             16831..17856
                     /gene="purR"
                     /locus_tag="ACHY03_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFS3904232.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17853..18785)
                     /gene="punR"
                     /locus_tag="ACHY03_000967"
     CDS             complement(17853..18785)
                     /gene="punR"
                     /locus_tag="ACHY03_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFS3904233.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            18898..20109
                     /gene="punC"
                     /locus_tag="ACHY03_000968"
     CDS             18898..20109
                     /gene="punC"
                     /locus_tag="ACHY03_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFS3904234.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            20400..21548
                     /gene="cfa"
                     /locus_tag="ACHY03_000969"
     CDS             20400..21548
                     /gene="cfa"
                     /locus_tag="ACHY03_000969"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFS3904235.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21588..22229)
                     /gene="ribE"
                     /locus_tag="ACHY03_000970"
     CDS             complement(21588..22229)
                     /gene="ribE"
                     /locus_tag="ACHY03_000970"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFS3904236.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22444..23817
                     /gene="mdtK"
                     /locus_tag="ACHY03_000971"
     CDS             22444..23817
                     /gene="mdtK"
                     /locus_tag="ACHY03_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFS3904237.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLAAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            complement(23858..25114)
                     /gene="ydhQ"
                     /locus_tag="ACHY03_000972"
     CDS             complement(23858..25114)
                     /gene="ydhQ"
                     /locus_tag="ACHY03_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFS3904238.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGLMQLTEGELIVEAGGRAENT
                     VVTGAGWLKVTTGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25422..25498
                     /locus_tag="ACHY03_000973"
     tRNA            25422..25498
                     /locus_tag="ACHY03_000973"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25456..25458,aa:Val,seq:gac)
     gene            25503..25579
                     /locus_tag="ACHY03_000974"
     tRNA            25503..25579
                     /locus_tag="ACHY03_000974"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25537..25539,aa:Val,seq:gac)
     gene            25687..25992
                     /gene="ydhR"
                     /locus_tag="ACHY03_000975"
     CDS             25687..25992
                     /gene="ydhR"
                     /locus_tag="ACHY03_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFS3904239.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTTRLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26118..27722
                     /gene="ydhS"
                     /locus_tag="ACHY03_000976"
     CDS             26118..27722
                     /gene="ydhS"
                     /locus_tag="ACHY03_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFS3904240.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLAFREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPATRARRRLSFD"
     gene            complement(27734..28546)
                     /gene="ydhT"
                     /locus_tag="ACHY03_000977"
     CDS             complement(27734..28546)
                     /gene="ydhT"
                     /locus_tag="ACHY03_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFS3904241.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRYFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERAFCSQFARSDDHARIGCCED
                     NVRIATAGYAVQIASMGYSVQIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(28550..29335)
                     /gene="phsC"
                     /locus_tag="ACHY03_000978"
     CDS             complement(28550..29335)
                     /gene="phsC"
                     /locus_tag="ACHY03_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFS3904242.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKIYLYSKAVRLWHWSNALLFVLLLASGLINHFAMLGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29332..30000)
                     /gene="ydhX"
                     /locus_tag="ACHY03_000979"
     CDS             complement(29332..30000)
                     /gene="ydhX"
                     /locus_tag="ACHY03_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFS3904243.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30064..30702)
                     /gene="ydhW"
                     /locus_tag="ACHY03_000980"
     CDS             complement(30064..30702)
                     /gene="ydhW"
                     /locus_tag="ACHY03_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFS3904244.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30715..32817)
                     /gene="ydhV"
                     /locus_tag="ACHY03_000981"
     CDS             complement(30715..32817)
                     /gene="ydhV"
                     /locus_tag="ACHY03_000981"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFS3904245.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(32838..33464)
                     /gene="ydhY"
                     /locus_tag="ACHY03_000982"
     CDS             complement(32838..33464)
                     /gene="ydhY"
                     /locus_tag="ACHY03_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFS3904246.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8061 a         8891 c         8297 g         8386 t
ORIGIN      
        1 aaggaatcta ctttgtgaag tttatgctca atgcaacagg attgccctta caagatctgg
       61 tgttcggtgc gtccgtctac tttcctccgt ttttcaaagc cttcgcgttt ggattcgtca
      121 tctggcttgt cgtacaccgc ctgcttcgtg gctggatcta cgccggtgac atctggcatc
      181 ccttgttaat ggatttatcg ctgtttgcga tttgcgtttg ccttgctctg gcaatactga
      241 ttgcgtggta actatgtcaa ttaaaacaat taagtatttc tcaacaatca ttgtagcgat
      301 agttgcggtt cttgccggat ggtggctgtg gaattattac atgcaatctc cgtggacacg
      361 cgacgggaaa atacgcgcag aacaggtttc tatcacccca caggtgtcgg gacgtattgt
      421 tgagctgaat ataaaagaca accagctggt taacgcgggg gatcttctgc tcacgatcga
      481 taaaacgccc tttcagatcg ccgagctgaa cgcacaggct cagctgtcga aagcgcaatc
      541 tgacctcgca aaagccaata acgaagcgaa tcgtcgtcgt catctctcac aaaattttat
      601 ttctgccgaa gaactcgata ccgctaacct caatgttaaa gcgatgcagg ccagtgttga
      661 tgccgcacag gcgacgctga aacaggcaca atggcaactg gcgcaaacgg aaattcgcgc
      721 cccggtgagt ggatgggtga ctaaccttac tacccgcatc ggcgattacg ctgataccgg
      781 gaaaccgctg tttgcccttg tcgacagcaa ctcgttttac gtcattggtt attttgaaga
      841 aaccaagttg cgccatatcc gcgaaggtgc accagcacaa attaccctct atagtgataa
      901 taaaacgtta cagggtcacg tttccagtat cggtcgggcg atttatgatc agagcgttga
      961 aagtgactca agccttattc cggatgtgaa acctaacgtc ccctgggttc gtctcgccca
     1021 acgcgttccc gtccgctttg cgctggataa agttccaggc gatgtcacgc tggtgtcggg
     1081 caccacctgt agcattgccg taggtcaata atgaacgcat cgtcatggtc cttgcgcaat
     1141 ttgccctggt tcagggccac gctggcgcaa tggcgttatg cgttacgcaa taccattgct
     1201 atgtgtctgg cgctgacggt tgcctattat ttgaatctgg atgaacccta ttgggcgatg
     1261 acctcggctg cagtggttag ctttcccacc gttggcggtg ttatcagcaa aagcctcgga
     1321 cgcatcgctg gcagtttgct cggagccatt gcggcactgc ttcttgccgg acatacactt
     1381 aatgagccgt ggttttttct attgagcatg gcggcgtggc ttggcttttg tacctgggcc
     1441 tgtgcgcact tcacgaataa tgtcgcgtat gcatttcaac tggcgggcta cacggctgcc
     1501 atcatcgcct ttccgatggt taatattact gaggccagcc agctgtggga tatcgctcag
     1561 gcgcgcgttt gcgaggtgat tgtcggtatt ttgtgcggcg gcatgatgat gatgatcctg
     1621 ccgagcagtt ccgatgctac tgccctttta accgcattga aaaacatgca cgcccgatta
     1681 ctggaacatg ccagtttact ctggcagcct gaaacaaccg atgccattcg tgcagcacat
     1741 gagggggtga ttggacagat actgaccatg aatttgctgc gtatccaggc tttctggagc
     1801 cactatcgtt ttcgccagca aaacgcgcgc cttaatgcgc tgctccacca gcaattacgt
     1861 atgaccagtg tcatctccag cctgcgacgt atgttgctca actggccctc accgccaggt
     1921 gccacacgag aaattctcga acagttgctg acggcgctcg ccagttcgca aacagatgtt
     1981 tacaccgtcg cacgtattat cgccccgcta cgcccgacca acgtcgccga ctatcggcac
     2041 gtcgccttct ggcagcgact acgttatttt tgccgccttt atctgcaaag tagtcaggaa
     2101 ttacatcgtc tgcaaagcgg tgtagatgat cataccagac tcccacggac atccggcctg
     2161 gctcgtcata ccgataacgc cgaagctatg tggagcgggc tgcgtacatt ttgtacgttg
     2221 atgatgattg gcgcatggag tattgcttcg caatgggatg ccggtgccaa tgcattaacg
     2281 ctggcagcga ttagctgcgt actctactcc gccgtcgcag caccgtttaa gtcgttgtca
     2341 ctgctgatgc gcacgctggt gttactttcg ctattcagct ttgtggtcaa atttggtctg
     2401 atggtccaga ttagcgatct gtggcaattt ttgttgtttc tctttccact gctggcgaca
     2461 atgcagcttc ttaaattgca gatgcccaaa tttgccgcgc tgtgggggca actgattgtt
     2521 tttatgggtt cttttatcgc cgtcactaat cccccggtgt atgattttgc tgattttctt
     2581 aacgataatc tggcgaaaat cgttggcgtc gcgttggcgt ggttagcgtt cgccattctg
     2641 cgtccaggat cggatgctcg caaaagccgc cgccatattc gcgcgctgcg ccgggatttt
     2701 gtcgatcaac taagccgcca tccaacactg agtgaaagcg aatttgaatc gctcacttat
     2761 catcacgtca gtcagttgag taacagccag gatgcgctgg ctcgccgttg gttattacgc
     2821 tggggtgtag tgctgctgaa ctgttctcat gttgtctggc aattacgcga ctgggaatcg
     2881 cgttccgatc cgttatcgcg agtacgggat aactgtattt cactgttgcg gggagtgatg
     2941 agtgagcgtg gcgttcagca aaaatcactg gcggccacac ttgaagaatt acagcggatt
     3001 tgcgacagcc ttgcccgtca tcatcaacct gccgcccgtg agctggcggc aattgtctgg
     3061 cggctgtact gctcgctttc gcaacttgag caagcaccac cgcaaggtac gctggcctct
     3121 taattactta attacaccac aggcatagcg ttcaccgcca ccgcccagcg gtttaggttg
     3181 atcggacata ttatcgccgc caacgtggac catcagcgct ttgtctttga tttcatccag
     3241 tgatttcaga cgaggcgcga tgacggcatc ggtagctttg ccgtcattat tgacgaccag
     3301 tgcaggcaga tcgcctaaat gcccggcacc ttctggccct tcatgtttac cggtattttg
     3361 tggatcaaga tgcccgcctg cggattccgc ggcgctggct ttgccatctt tggtggctgg
     3421 ctggcagctt cctttggcat gaatatggaa gccatgttca ccggggggta atgctttcag
     3481 atcgggcgaa aactccagac ctttatcggt ttcagtaatg gtgacgctac caattgactg
     3541 cccaacccct tgcgacgtga cgaggttcat ctcgactttt tcactggcag cttgtgcgcc
     3601 ggtcgcaaca accagcgcca gaatggccag actaaaacgt ttcataggac ctccgttcat
     3661 tgtgcattcc tgaataagtg taaaccagtg acacattttt gaacggcggc tattcctaaa
     3721 agtgcttacg gtacgtcgta ccccagtgcc gctttacgga tacgaaacca ttgttgacgg
     3781 gtcattttca gtgtttctgc ttcgacagct gcccgtacgc gctcaatttt acctgagccg
     3841 ataattggca gcggctgcga cggtaaacgt aacacccagg cgtaaaccac ctgttcaatc
     3901 gagcccgcgt ttaactcctc cgccaccaca gccagttcat cacgtagcgg ctggaaataa
     3961 tcatcattaa acagacgacc accaccaagg caggaccacg ccatcggacg aacacgcagt
     4021 tgttgcagtt ggtcgagcgt gccatccagc agtaacggct gatgtaccgg ggatatttcc
     4081 acctgattag tggcaagggt aaacggcaga cgtgattgca acagggcaaa ttgcgcaggc
     4141 gtaaagttcg atacgccaaa atgacgcact ttgccgctct gatgcagatg tttgaacgcg
     4201 tccgccactt catcggcatc cattaacggg tccgggcggt ggattaacag cagatccaga
     4261 tgatcggtcg caagattaat tagcgactgt tcggcgctct taatgatgtg atcgcggtca
     4321 gtgatgtaat gaccaatgac gttttcttca cgcgcggtcg tcgcgatacc gcatttactg
     4381 acgatttcca tccgctcacg caggtgaggt gccagtttca gcgcctcgcc aaacgccgct
     4441 tcgcactgat agccaccata aatatcagca tggtccacgg tggtcacgcc gagatccaga
     4501 tgctcttcaa taaaactgac cagctggcgg gcggacatgt tccagtccat caatcgccag
     4561 tagcccatca caaaacggga aaactccggg ccttgcggcg caatagtaat acgctgaacc
     4621 ataatcgctt cctcttatca gatatgagag gagtatacgc aagattaggc tcaaaagagt
     4681 gatggttgct ccggttcgtc tgatgacgct ggcttatttg cacgtaattt gcgcattaat
     4741 cgttgccgac aaaggcgcag cacctcttgt ttttcgccat cgctcatttt attccagtta
     4801 aaacgctcat cccgactacg aaaacagccg cgacaaaatc cgcgttcgtc agactgacaa
     4861 attccccggc acgggctctg gacgggaaag aactctaatt gctccgccac ttcgccctcc
     4921 tcaaataaga ttattaccat tattgaagct gttaatgccc aaagtagcaa ctttgcttgc
     4981 actagaccga ctggtctact acactccaac gcatgaacaa acacaccgaa catgatactc
     5041 gcgaacatct cctggcgacg ggcgagcaac tttgcctgca acgtggattc accgggatgg
     5101 ggctaagcga attactaaaa accgctgaag tgccgaaagg gtccttctat cactactttc
     5161 gctctaaaga agcgtttggc gtagccatgc ttgagcgcca ttacgccgca tatcaccagc
     5221 gactgactga gttgctgcaa tccggcgaag gtaactaccg cgaccgcata ctggcttatt
     5281 accagcaaac actgaaccag ttttgccaac atggaaccat cagtggttgc ctgacagtaa
     5341 aactctctgc cgaagtgtgc gatctgtcag aagatatgcg tagcgcgatg gataaaggcg
     5401 ctcgcggcgt gatcgccctg ctctcgcagg ctctggaaaa tggccgtgat agccattgtt
     5461 taaccttttg tggcgaaccg ctgcaacagg cacaagtgct ttacgcacta tggctgggtg
     5521 cgaatctgca ggccaaaatt tcgcgcaatt ccgagccact ggaaaacgcg ctggcacatg
     5581 taaaaaccat tattgcgacg cctgccgttt agcaggcatt ttttatcacc agacgaccgg
     5641 gagcctttat gtcatctgaa aaactgtatt ccccactgaa agtgggcgca ataactgcgg
     5701 caaaccgtat ttttatggca ccgctgacgc gtctgcgcag tattgaaccg ggtgacatcc
     5761 ctaccccgtt gatggcggaa tactatcgcc aacgtgccag tgccggtttg attattagcg
     5821 aagccacgca aatttctgcc caggcaaaag ggtatgctgg tgcacctggc atccatagtc
     5881 cggaacaaat tgccgcatgg aaaaaaatta ccgctggcgt tcatgctgaa aatgggcata
     5941 tggccgtaca gctgtggcac accggacgca tttctcacgc cagcctgcaa cctggcggtc
     6001 aggcaccggt agcgccttca gcacttagcg cgggaacacg tacttctctg cgcgatgaaa
     6061 atggtcaggc gatccgtgtt gaaacatcca tgccgcgtgc gcttgagctg gaagagattc
     6121 caggtatcgt caatgatttc cgtcaggcca ttgctaacgc gcgtgaagcc ggttttgatc
     6181 tggtagagct ccactctgct cacggttatt tgctgcatca gttcctttcc ccttcttcaa
     6241 accatcgtac cgatcagtac ggcggcagcg tggaaaatcg cgcacgtttg gtactggaag
     6301 tggtcgatgc cgggattgaa gaatggggtg ccgatcgcat tggcattcgc gtttcgccaa
     6361 tcggtacttt ccagaacaca gataacggcc cgaatgaaga agccgatgca ctgtatctga
     6421 ttgaacaact gggtaaacgc ggcattgctt atctgcatat gtcagaaccg gattgggcgg
     6481 gaggtgaacc gtatactgat gcgttccgcg aaaaagtacg cgcccgtttc cacggtccga
     6541 ttatcggcgc aggtgcatac acggtagaaa aagccgaaac gctgattggc aaagggttaa
     6601 ttgatgcggt ggcatttggt cgtgactgga ttgcgaaccc ggatctggtc gcccgcttgc
     6661 agcgcaaagc tgagcttaac ccacagcgtg ctgaaagttt ctatggtggc ggtgcggaag
     6721 gctacactga ttacccgacg ttgtaatcca acattgcgag cggcgcaaag ccgccgctat
     6781 actaaaacaa cattttgaat ctgttagcca ttttgaggat aaaaagatgc gtcttcttca
     6841 taccatgctg cgcgttggcg atctgcaacg ctccatcgat ttttatacca aagtgctggg
     6901 catgaaactg ctgcgtacca gcgaaaaccc ggaatacaaa tactcgctgg cgtttgttgg
     6961 ctacggcccg gaaaccgaag aagcggtgat tgaactgacc tacaactggg gcgtggataa
     7021 atacgaactc ggcaccgctt atggtcacat cgcgcttagc gtagataatg ccgctgaagc
     7081 gtgcgaaaaa atccgtcaaa acgggggtaa cgtgacccgt gaagcgggtc cggtaaaagg
     7141 cggtactacg gttatcgcgt ttgtggaaga tccggacggt tacaaaattg agttaatcga
     7201 agagaaagac gccggtcgcg gtctgggcaa ctaatctcct gccgggcgcg aactcatcgc
     7261 gcccgcatct ttactgcatc gacaagtaat atttgtcata atgcgcgctg caatttatcc
     7321 gtattaagag aatcagatgt ccgataacgc tcaacttacc ggtctgtgcg accgttttcg
     7381 tggtttttat cctgttgtga tcgatgttga aacagccgga tttaacgcca aaaccgatgc
     7441 gctgcttgag attgccgcca tcaccctgaa aatggatgaa caaggctggc tgatgccgga
     7501 caccacatta catttccacg tcgaaccatt tgtcggcgca aatttgcaac cagaagccct
     7561 tgccttcaac ggcattgacc cgaacgatcc cgatcgcggc gcggtcagcg aatacgaggc
     7621 gctgcacgaa atttttaaag ttgtacgtaa aggtattaaa gcgagcggct gtaaccgcgc
     7681 cattatggtg gcgcacaacg ccaatttcga tcacagcttt atgatggccg ccgctgaacg
     7741 cgcctcactg aaacgtaacc cgttccaccc tttcgccact tttgacactg ctgcactggc
     7801 cgggctggca ctcggacaaa ccgtattgtc aaaggcttgc cagaccgctg gcatggactt
     7861 cgacagcacc caagctcact ccgcgctgta cgacaccgaa cgcactgcgg tgctgttttg
     7921 tgaaatcgtc aaccgctgga aacgtctggg aggctggccg ctacctgccg ccgaagaggt
     7981 gtaatcgagt cgatgcctga tgacatgcaa tgattcaggc atctatagtg aggctattcc
     8041 acgcatcatg catgatattc acggggaata gcgttaatgg cagataatcc aaacccttca
     8101 tcgcccctgc cggacgtgtt ttcaccggcg acccgcgact ggtttcttcg cgcctttaaa
     8161 cagccgaccg ctgtccagtc gcaaacctgg catgtggcgg cgcgaggcga acatgcgctg
     8221 gtgattgcac cgaccggctc cgggaaaacg ctggcagcat ttctctacgc cctcgatcgg
     8281 ctcttccgcg aaggcggcga agatacccgc gaggcgcata agcgtaaaac ctcacgcatt
     8341 ctctatattt caccgataaa agccctgggc accgacgttc agcgcaactt gcagatcccg
     8401 ttacagggta ttgccgatga acggcggcgg cgcggcgaaa cggaagtcaa tcttcgcgtg
     8461 gggatccgta ctggcgatac gcctgcacag gaacgcagca aactcacccg taatccgccg
     8521 gatattctga tcaccacacc cgaatcactc tatctgatgc tgacctcccg cgcgcgcgaa
     8581 acgctacgcg gcgtcgaaac ggtaattatt gatgaagtcc acgcggtagc gggcagtaaa
     8641 cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca cacctcagca
     8701 cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc atttcttggc
     8761 ggcgatcgcc cggttacggt agtcaacccg cccgcaatgc gccatccgca gatacggatt
     8821 gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac tagcgaagac
     8881 agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat ccttgatgaa
     8941 gtgttgcgcc atcgctcgac cattgtcttt actaattcgc gggggctggc ggaaaaactg
     9001 acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc tatcgccgtt
     9061 gatgcggccc atttcgagtc gacctctggc gcaacctcta accgtgtaca aagtagcgac
     9121 gtttttattg cccgctcaca ccacggctct gtctctaaag aacaacgagc aatcaccgag
     9181 caggcgctga aatcgggtga attacgctgc gtggtcgcaa cttccagtct cgaactgggg
     9241 attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc tgttgccagt
     9301 gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa agggctgttt
     9361 ttcccccgaa cccggcgtga cttagtcgat tccgcagtca ttgtagagtg tatgttcgca
     9421 ggcaggctgg aaaacctgac acctccgcat aatccactcg acgtccttgc gcagcaaacc
     9481 gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg cgtacgccgt
     9541 gccgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga tatgctttcc
     9601 gggcgctatc cctctggcga tttttctgct tttcgaccaa aactggtctg gaaccgggag
     9661 accgggatat tgtccgcccg acctggcgct cagctgttgg cggttaccag cggtggcacc
     9721 attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaca ggccggttcg
     9781 cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa cgacattatc
     9841 actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt gattgtgact
     9901 cctgctccgg gtcgttctgc ccggctcccc ttctggcgtg gtgaaggtaa cggacgtccg
     9961 gctgaattag gcgagatgat cggcgatttt cttcatttgc tggcggatgg cgcgttcttt
    10021 tccgggacta ttcccccgtg gctggctgaa gaaaatacga tctccaatat tcaggggttg
    10081 attgacgagc agcgcaacgc gacgggcatc gttccgggga gtcgtcatct ggtcctcgaa
    10141 cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta tggaagacgg
    10201 gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgcgctatg gggcgctgac
    10261 gcgtctgtgg tcgccagtga tgacggcatt gttgcgcgca ttcctgacac tgatggtaaa
    10321 ttgcccgacg ccgcgatttt tttgtttgaa ccagaaaagt tgctgcaaat tgtccgcgag
    10381 gcggtaggca gctcagcact tttcgccgcc cgttttcgcg aatgcgccgc gcgggcgtta
    10441 ttaatgccgg gccgcacgcc gggccatcgc accccgctct ggcaacaacg gctgcgcgcc
    10501 agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct cgaaacccta
    10561 cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat gcgtcgcctg
    10621 aacggtggcg aaattcaaat atctgatgta acgactacta cgccctcgcc tttcgccaca
    10681 agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc gctggcagag
    10741 cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact cggacaggtc
    10801 gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga gttgcaacga
    10861 ctggctcctg gcagaagagc gaaaggtgaa gaaggattgt tcgacctgct gcgcgaactg
    10921 gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga agagattgcg
    10981 tcgtatctgg aaaatcttct tacagtaaaa cgaatcttcc cggcgatgat tagcggacag
    11041 gagcgtcttg cctgtatgga tgatgccgcc aggctgcgcg atgccctcgg cgtacaacta
    11101 ccagagtctc tgccagcgat ttatttgcat agagtcagtt acccgcttcg cgacctcttt
    11161 ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca tgagtttagt
    11221 ctcggtattg ccattgtcga agagcagctt cagcaactgc gcgaacaggg tctggtgatg
    11281 aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg tttgcgctcg
    11341 ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc gcgattgctg
    11401 ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt tgcctcaaca
    11461 tcgccaggcg tttatgaggg cgtagatggc gtgatgcggg tgatcgaaca gcttgccgga
    11521 gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt acgcgactat
    11581 tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc gggacaaaaa
    11641 aagctgggtg aagatgacgg cctggtggca ctgcatctac aggaatatgc cgcagaatcg
    11701 ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc gatagtcgct
    11761 gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat acgcgataaa
    11821 atcggcgaat cggttgatcc ctcggcactg caagaggcgt tatgggcgct ggtctggcaa
    11881 ggcgtcatca ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag cagttccaac
    11941 gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt ctatgcacaa
    12001 cccgtctcgc cgctggtgtc ttacaacaca ccaaatctgg ctggacgctg gtcgttattg
    12061 caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa tatgctcgac
    12121 cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg cgggtttcca
    12181 tcgatgcaaa cgctttgtcg aagtatggaa gattccgggc gaattatgcg aggtcgtttt
    12241 gtagaaggtc tgggtggcgc acaattcgct gaacgtctga ctattgaccg cttgcgcgac
    12301 ctggcgacac aagccgcgca aacgcgccac tatacaccag tggcgctctc cgccaacgat
    12361 ccggctaatg tgtggggaaa tcttctgccc tggcctacac atccggcaac gctggtacca
    12421 acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact ctatctggcg
    12481 caaggtggca aaaaaatgct ggtctggcag gaaaaagagg aattactcgc cccagaggtt
    12541 ttccacgcgc tgactaccgc actgcgtcgc gaaccacggc tgcgctttac gctaacagaa
    12601 gtgaatgatc taccggtccg gcaaacgccg atgtttacgc tgctgcgtga ggcgggattt
    12661 tcaagttcgc cacaagggct ggattgggga tagataaagg actgacggat gcccgttcgc
    12721 atccgtcagt attgcaggac ggattattcc gcgtccggct cttcagactt gtatttagcg
    12781 gcagtttctt tgatcagctg ctgcagttcg ccacgctgat acatttcgat cacgatatca
    12841 caaccgccga ccagctcgcc gtcaacccac agttgcggga aggtcggcca gttagcatat
    12901 ttcggcagtt ccgcacgaat gtccggattc tgcagaatat caacataggc aaaacgttcg
    12961 ccacatgcgg caagcgcctg gactgcctgg gcagagaaac cgcagctcgg cagtttcggt
    13021 gaacctttca tgtacagcag gatcgggttt tcagcaatct ggcgttggat tttttcgata
    13081 gtggtgctca ttgtcttgct tcctcaaact tctttacggc agtaatctga cattgtagcg
    13141 ggtcagtgcg gcatcggaaa ataacatttt catcacgctt ttgctatttt atccctttgc
    13201 cctatttgtt gcatttcaaa tattcgtttc ttccctgatg cacggcaaac tggcgtattt
    13261 atgaccattt ttagttttca ctgctgcatt tttttgcgct cgccaacgaa acgtattttt
    13321 taacaataaa agctattaac tttctcttct tctatgcatt agaatcatca agttttgtaa
    13381 atcagacgca ggcatgatag acctgcctta acagagggac gctcagtggc gcggataaac
    13441 cgtatttcga tcacgctctg tgctttactt tttaccaccc tgcctttaac gcctatggcc
    13501 catgcttcaa agcaagccag ggagagttct gctaccactc atatcaccaa aaaagcagat
    13561 aaaaagaaaa gcacggcaac caccaaaaag acccagaaag ccagccagaa aacagcgaaa
    13621 aaagccgcca gtaaaagtac gaccaaaagc aaaaccgctt cttccgttaa aaaatcttcc
    13681 attaccgctt ctaaaaacgc caaaactcgc agcaaacata ccgtcaataa aacggcctca
    13741 gccagcttta ccgaaaagtg taccaaacgt aagggctata aatcgcattg tgtgaaagtc
    13801 aaaaatgcag cgtcaggaac ccttgccgac gcacacaaag cgaaggtgca aaaagccaca
    13861 aaagtggcaa tgaataaact gatgcagcaa attggtaagc catatcgttg gggtggcagc
    13921 tcaccgcgta ccggttttga ttgcagcggc ctggtttatt acgcttataa agatttggtg
    13981 aaaattcgaa ttccgcgtac ggcgaatgaa atgtatcacc tgcgtgatgc agcacctatc
    14041 gaacgtagtg agctgaaaaa cggcgacctg gtctttttcc gtactcaggg acgcggtaca
    14101 gccgatcatg tcggcgtgta tgtcggcaac ggcaaattta ttcagtcacc gcgcacaggt
    14161 caggaaattc aaatcacttc tctcagtgaa gactactggc agcgccacta tgttggcgct
    14221 cgccgggtaa tgaccccaaa aacacttcgc taaaacttta ccctgttgtt acggcaacag
    14281 ggtaagttca tcttttgtct caccttttaa tttgctaccc tatccatacg cacaataagg
    14341 ctattgtacg tatgcaaatt aataataaag gagagtagca atgtcattcg aattacctgc
    14401 actaccatat gctaaagatg ctctggcacc gcatatttct gcggaaacca tcgagtatca
    14461 ctacggcaag catcatcaga cttatgtcac taacctgaac aacctgatca aaggtaccgc
    14521 gtttgaaggt aaatcactgg aagagattat tcgcagctct gaaggtggcg tattcaacaa
    14581 cgcagctcag gtctggaacc atactttcta ctggaactgc ctggcaccga acgccggtgg
    14641 cgaaccgact ggaaaagtcg ctgaagctat cgccgcatct tttggcagct ttgccgattt
    14701 caaagctcag tttactgatg cagcgatcaa aaactttggt tctggctgga cctggctggt
    14761 gaaaaacagc gatggcaaac tggctatcgt ttcaacctct aacgcgggta ctccactgac
    14821 caccgatgcg actccgctgc tgaccgttga tgtctgggaa cacgcatatt acatcgatta
    14881 tcgcaatgca cgtcctggct atctggagca cttctgggcg ctggtgaact gggaattcgt
    14941 agcgaaaaat ctcgctgcat aataactgat ggcaaatgca gcattgcctg atgcgcttcg
    15001 cttaccatgc caacgcggat gaggcgttaa cagcgcattc ggcaagataa atcgcacgtt
    15061 gtcagcaact gtaacgcaga aggttatcct tctgcgtttt tgtttaatta gctgttagca
    15121 acgcaaactg tttcaggttg ttttctggct gacataaaca ccagcaataa tgccagtccc
    15181 gcgacaattg cccccatcac cggcacaaag ctgtatccca gcccagcgga aattaccgca
    15241 ccaccagcag ctgctcccag cgcatttcca agattaaagg caccaatatt gactgatgaa
    15301 gacagacctg gcgcttcact ggcgacacgc atcacgcgca tctgtaacgg cggtacgacc
    15361 gcaaaggttg cagcgcccca caccaccatg ctaatagctg cgccgaactc attgcgggcc
    15421 aggaacggga ttaccagcat aatcaccatc aacaacaaca aaaagccttt caacgtgccg
    15481 ttaactgaac gatctgcaag tttgccgccg agatagttac cgatagagaa tccgacacca
    15541 atcagcacca gcattgccgt gacgaacacc ggtgttgcgt gggcaatact ttgcagtacc
    15601 ggagagatat aggtgtagag ggtaaacatt gcaccagctc ccagtaccgt cgtcagcaat
    15661 gcagacagca cctgcggacg cattaatacc gccagctctt ttttcacttc aggtcgcgcc
    15721 cctgcaccac ctttaggtaa tgagaagaac agacttacca ttgaaatcac gcccagcccc
    15781 gccgtagcca gaaatgacat ccgccagccg atggtttcac ccaaccaggt cgccgccggc
    15841 acgccaccga tatttgccag ggttaacccc ataaacatag tggcaactgc gctggcctgt
    15901 ttatgttttg gcaccacgct tgcagccacg actgaaccca aaccaaaaaa tgctccgtga
    15961 ttcaggctgg tcaaaatgcg tgaaagcatc agggtcatat aatccggcgc gatggcggaa
    16021 agtacgttgc cgagcgtgaa aattgccatc aggaaaatca acgcactgcg gcgggcacga
    16081 tgagaaagta gaagcgtcat cagcggcgcg ccaaccatta cgccaactgc ataggcactg
    16141 attaacattc cggcagcggg aatcgagaca tccacaccgc gcgcaatgac gggcaacaag
    16201 cccattggcg agaactccgt tgtcccgata ccaaacgcgc caatcgccag cgccagcaac
    16261 ggatagttaa ttttcatgcc ttatctccac ctcttcgcgt cattacgcga tattcattaa
    16321 agtggcgaaa gcatgacagc aatcacaaaa aaatgaaaat aacaaaaaga gaaaacactt
    16381 ttgccatttt gctaacaaac aggaaggaga tgcgagggag aacgcgctcc ctcgagagga
    16441 aatcagtgca gcgcggcagt caaacccacg gccacgatca aaccgaggac gataatcgtt
    16501 gttaccagtg aaaatttaag gtcggtgctc atcaagtttt ctcctttttt attaccacac
    16561 aaaaagtgat attacgcatt tttacacact gtgatgaaaa aatctcccgt catttataat
    16621 gataagtgtt tttaccactt ccccttttcg tcaagatcgg ccaaaattcc acgcttacac
    16681 tatttgcgta ctggccattg accccttcct gacgctccgt gtcgtttttc cggcgtaccg
    16741 caacactttt gttgtgcgta aggtgtgtaa aggcaaacgt ttaccttgcg attttgcagg
    16801 agctgaagtt agggtctgga gtgaaatgga atggcaacaa taaaagatgt agcgaaacga
    16861 gcaaacgttt ccactacaac tgtgtcacac gtgatcaaca aaacacgttt cgtcgctgaa
    16921 gaaacgcgca acgccgtgtg ggcagcgatc aaagaattac actactcccc tagcgcggtg
    16981 gcgcgtagcc tgaaggttaa ccacaccaag tctatcggtt tgctggcgac cagcagcgaa
    17041 gcggcctatt ttgccgagat cattgaagca gttgaaaaaa attgcttcca gaaaggttac
    17101 accctgattc tgggtaatgc gtggaacaat cttgagaaac agcgggctta tctgtcgatg
    17161 atggcgcaaa aacgcgtcga tggtctgctg gtgatgtgtt ctgagtaccc agagccgttg
    17221 ctggcgatgc tggaagagta tcgccatatc ccaatggtgg tgatggactg gggtgaagca
    17281 aaagctgact tcaccgatgc ggtcattgat aacgcgttcg aaggcggcta catggccggg
    17341 cgttatctga ttgaacgcgg tcaccgcgaa atcggcgtta tccccggccc gctggaacgt
    17401 aacaccggcg caggccgcct tgccggtttt atgaaggcga tggaagaagc gatgatcaaa
    17461 gtgccggaaa gctggattgt gcagggtgac tttgaacctg aatccggtta tcgcgccatg
    17521 cagcaaatcc tatcgcagcc gcatcgccct actgccgtct tctgtggtgg cgatatcatg
    17581 gcaatgggcg cactttgtgc tgctgatgaa atggggctgc gcgtcccgca ggatgtttcg
    17641 ctgatcggtt atgataacgt gcgcaacgcc cgctatttta cgccggcgct gaccacgatc
    17701 catcagccaa aagattcgct gggtgaaaca gcgttcaaca tgctgttgga tcgtatcgtc
    17761 aacaaacgtg aagaaccgca gtccattgaa gtgcatccgc gcttgattga acgccgctcc
    17821 gtggctgacg gcccgttccg cgactatcgc cgttaatcac ctgttgcggg agtctcttcc
    17881 ggctcccgca gccactcctt attcagcgtc tcactatcgc cgagatactc aagcaaccag
    17941 gttaacgcag gcgacatatc attttgctgc catgtcagac aacatgccga atccgggaag
    18001 gggttttcca attcaagtgc tacccacttc ccctcattaa gccacggttt ggcgaaatgt
    18061 gttggcacca tccctatgca taatcctgcc gagatacagg ttgccgatga ttcccagtca
    18121 ggcacgacaa ctcttttttg gttatccagc aaccaggtaa tacgtttagg tagcgttcgc
    18181 gaggtgtctt cgcgcaccaa cgacggccag ttgcgcaacg tatcatcgct gaacgggcca
    18241 tccatcaacg ccagcgggtg gtgactggca acaacgcaac tccagcttag catccccata
    18301 tcccggaagg cataacgacc gcctaccgga atcgcgcgtg ttgcgccaat cgccagttcc
    18361 acgcgcccgt cggaaagcgc atcccagaca ccgttgaaca cttcctgaaa gacaagaagt
    18421 tcgacatcat caaaatggcg ataaaaatca acgatcatct gccgtgtacg ttctggcctg
    18481 acaatattat ccactgcgat agctaactga ccgcgccagc cgttcgctat ctgctgacat
    18541 tgctggcggg tgatctgcat ttttttgaca acagagcgcc cttctttgag aaaccacgct
    18601 ccagcagcgg tcagctccac atcacggtgc cgtcgttcaa agagcggcac cgccagccac
    18661 tcttccagct gacgcacggt atagctgacc gcagaaggaa cgcgatgcag ctcctgtgcc
    18721 gcagcgctaa aactaccatt acgcgctacc gcatcaacaa cttcgagtga atattctgac
    18781 cacatagtct gcctgcaaaa tttttgaaac cagtcatcaa atattaccgt ttcacaacac
    18841 taatttcact ccctacactt tgcggcggtg tttaattgag agatttagag aatatacatg
    18901 caacctggga aaagattttt agtctggctg gcgggtttga gcgtactcgg ttttctggca
    18961 accgatatgt atctgcctgc tttcgccgcc atacaggccg acctgcaaac gcctgcgtct
    19021 gctgtcagtg ccagccttag tctgttcctt gccggttttg ccgcagccca gcttctgtgg
    19081 gggccgctct ccgaccgtta tggtcgtaaa ccggtgttat taatcggcct gacaattttt
    19141 gcgttaggta gtctggggat gctgtgggta gaaaacgccg ctacgctgct ggtattgcgt
    19201 tttgtacagg ctgtgggtgt ctgcgccgcg gcggttatct ggcaagcgtt agtgacggat
    19261 tattatcctt cgcagaaagt taaccgaatt tttgcgacca tcatgccgct ggtgggtcta
    19321 tctccggccc tggctcctct gttaggaagc tggctgctgg tccatttttc ctggcaggcg
    19381 attttcgcca ccctgtttgc cattaccgtg gtgctgattc tgcctatttt ctggctcaaa
    19441 cccacgacga aggcccgtaa caatagtcag gatggtctga cctttaccga cctgctacgt
    19501 tctaaaacct atcgcggcaa cgtgctgata tacgcggcct gttcagccag tttttttgca
    19561 tggctgaccg gttcaccgtt catccttagt gaaatgggct acagcccggc agttattggt
    19621 ttaagttatg tcccgcaaac tatcgcgttt ctgattggtg gttatggctg tcgcgccgcg
    19681 ctgcagaaat ggcaaggcaa gcagttatta ccgtggttgc tggtgctgtt tgctgtcagc
    19741 gtcattgcga cctgggctgc gggcttcatt agccatgtgt cgctggtcga aatcctgatc
    19801 ccattctgtg tgatggcgat tgccaatggc gcgatctacc ctattgttgt cgcccaggcg
    19861 ctgcgtccct tcccacacgc aactggtcgc gccgcagcgt tgcagaacac tcttcaactg
    19921 ggtctgtgct tcctcgcaag tctggtagtt tcctggctga tcagtatcag cacgccattg
    19981 ctcaccacca ccagcgtgat gttatcaaca gtagtgctgg tcgcgctggg ttacatgatg
    20041 caacgttgtg aagaagttgg ctgccagaat catggcaatg ccgaagtcgc tcatagcgaa
    20101 tcacactgat ctatatcgat atacttatac ttaggctgct aacaaaattt tgttgtatga
    20161 ttgaaattag cggcctatac taatttcgag ttgttaaagc tacgataaat attatgtttt
    20221 tacggggaca ggatcgttcc cgactcacta tggatggtca tttcggcaag ggttcctcct
    20281 ttccctctgt tctacgtcgg attatagact cgcggttttt tctgcgagat ttctcacaaa
    20341 gcccaaaaag cgtctacgct gttttaaggt tctgatcacc gaccagtgat ggagaaacta
    20401 tgagttcatc gtgtatagaa gaagtcagtg taccggatga caactggtac cgtatcgcca
    20461 acgaattact tagccgtgcc ggtatagcca ttaacggttc tgccccggcg gatattcgtg
    20521 tgaaaaaccc cgattttttt aaacgcgttc tgcaggaagg ctctttgggg ttaggcgaaa
    20581 gttatatgga tggctggtgg gaatgtgacc gacttgatat gttttttagc aaagtcttac
    20641 gcgcaggtct cgagaaccaa ctcccacatc atttcaaaga cacgctacgt attgccggcg
    20701 ctcgtctctt caatctgcag agtaaaaaac gtgcctggat agtcggcaaa gagcattacg
    20761 atttgggtaa tgacttgttc agccgcatgc ttgatccctt catgcaatat tcctgcgctt
    20821 actggaaaga tgccgataat ctggaatctg cccagcaggc gaagctcaaa atgatttgtg
    20881 aaaaattgca gttaaaacca gggatgcgcg tactggatat tggctgcggc tggggcggac
    20941 tggcgcacta catggcgtct aattatgacg taagcgtggt tggcgtcacc atttctgccg
    21001 aacagcaaaa aatggctcag gaacgctgtg aaggcctgga tgtcaccatt ttgctgcaag
    21061 attatcgtga cctgaacgac cagtttgatc gtattgtttc tgtggggatg ttcgagcacg
    21121 tcggaccgaa aaattacgat acctattttg cggtggtgga tcgtaatttg aaaccggaag
    21181 gtatattcct gctccatact atcggttcga aaaaaaccga tctgaatgtt gatccctgga
    21241 ttaataaata tatttttccg aacggttgct tgccctctgt acgccagatt gctcaatcca
    21301 gcgaacccca ctttgtgatg gaagactggc ataacttcgg tgctgattac gatactacgt
    21361 tgatggcgtg gtatgaacga ttcctcgccg catggccaga aattgcggat aactacagtg
    21421 aacgctttaa acggatgttt acctattatc tgaatgcctg cgcaggtgct ttccgcgccc
    21481 gtgatattca gctctggcag gtggtgttct cacgcggtgt tgaaaacggc cttcgagtcg
    21541 ctcgctaaag gctattctat cgccccctct ccgggggcga tttcagatca ggcttctgta
    21601 cctggttgat tcatggcatt ttctcgtgcc gccagcacac gttctaccgt atctaccact
    21661 gcctgagttt gtggatcgat ttcaatgttg acgcgtgcgc caagtttttt cttcccaaga
    21721 gtcgtgcgtt ccagtgtttc cggaattaaa tggacgcaaa aacgcgttgg cgtgacttcg
    21781 ccgacggtca ggctaatacc gtcgatgcca ataaatcctt tgtacaggat atatttcatc
    21841 aactgactat cctggacttt aaaccagatc tggcgattat tttccgaggt taatattttc
    21901 gccacttcag cggtggtcat aatatgacct gacattaagt gtccgccaat ttcatcactg
    21961 aatttcgccg cacgctcaac gtttacccaa tcccccactt ttaaatcgcc aagattggta
    22021 atgcgtaacg tttctttcat caggtcaaaa ctgacatggt tgccgttaat ttccgtcacg
    22081 gtcaggcagc aaccgttatg cgccacggaa gcaccggttt ccagaccgtc cagcatgtgg
    22141 tcgggtaact ccaccacatg tgtgcgaaaa tttggtttct cgtcaatcga caccagtttt
    22201 gcggtgccct gtacaatccc cgtaaacata cttacaactc ctgaaatcag ttaagacatt
    22261 ctgttcagca caatagcagg tggaaaacgc ccttaccagt gaaggggtaa gaatgactat
    22321 tttttcactg gagaattaat aaatcctcgc tacaatagac tgaatttccc ctgcttcttc
    22381 tttttgctgc ccattcaggc ggctttttag tctctcatat aactacaaat aaaaggtgtt
    22441 cacgtgcaga agtatatcag tgaagcgcgt ctgttattag cattagcaat cccggtgatt
    22501 ctcgcgcaaa tcgcccaaac tgcgatgggt tttgtcgata ccgtgatggc gggcggctat
    22561 agtgccaccg acatggcggc ggtcgctatc ggtacttcta tctggcttcc ggcgatcctc
    22621 tttggtcacg gactgctgct ggcattaacg ccggttatcg cgcaattaaa tggttccggt
    22681 cgacgtgagc gcattgcgca tcaggtgcga caaggtttct ggctggcagg ttttgtttcc
    22741 gttctcatta tgctggtgct gtggaatgca ggttacatta tccgctccat ggaaaacatc
    22801 gatccggctc tggcggacaa agccgtgggt tatctgcgtg cgttgctgtg gggcgcgcca
    22861 ggctatcttt tcttccaggt tgcccgtaac caatgtgaag gtctggcaaa aaccaagccg
    22921 ggtatggtaa tgggctttat cggcctgctg gtgaacatcc cggtgaacta tatctttatt
    22981 tatggtcatt tcggtatgcc tgagctcggt ggcgttggtt gtggcgtggc tactgcggcg
    23041 gtgtattggg tcatgttcct tgccatggtt tcttacatta aacgcgcccg ctcgatgcgc
    23101 gatattcgta acgaaaaagg caccgcgaaa cccgatcctg cggttatgaa acgactgatt
    23161 caactcggtt tgccgattgc gctggcgctg ttctttgaag tgacactgtt tgccgtcgtg
    23221 gctctgttag tgtctccgct cggtattgtt gatgtcgcag gacaccagat tgccctgaac
    23281 tttagttcac taatgttcgt gcttccaatg tcgctggcgg cagcggtaac tatccgcgta
    23341 ggttatcgtc tgggtcaggg ctcaacgctg gctgcgcaaa ccgctgcgcg gaccgggctt
    23401 atggtgggtg tctgtatggc aaccctgacg gccatattca cggtttcact gcgggagcaa
    23461 atcgccctgt tgtacaacga caatcccgag gttgtaacgc tggctgcgca tttgatgttg
    23521 ctggcggcgg tatatcagat ttctgactca atccaagtga ttggcagtgg gattttgcgt
    23581 ggttataaag atacgcgttc cattttctat atcaccttta cggcttactg ggtgctgggc
    23641 ttgccaagcg gctatattct ggcactgacc gatctggtcg ttgaacctat ggggccagca
    23701 ggcttctgga taggctttat tattggcctg acgtcggcag ccattatgat gatgctgcgt
    23761 atgcggttcc tgcaacgtct gccgtcagcc atcattctgc aacgagcatc ccgctaataa
    23821 agacaaggcg caaccttcgc aggttgcgcc tgtattttta cgcaggctgg agcgttgcgc
    23881 caatcccgtc ttcgtctggc tgtagtttca gtgcgttaca cagagtttgc cgactggcat
    23941 ctgttatctc aacgtgtata tccccggtaa cttccccttt cacattgatg aagtcaggtg
    24001 aaatttcttt cgcactggtt tgcaggcaaa tcgctccctc tccgcgcagg cgtttcactt
    24061 tcagctgcac atgcgttgcc gcaccagtga tcagccacag ttttccgcca ttcagattga
    24121 tgttaccaat actgaaagtg acactgtcac cttttaccgt actgccgtcg agaatcaata
    24181 caccgccctg ctgtacagta atcgttgatt gttgcagatc tgcgccatag ccaataatca
    24241 ttgaagcgcc gtccagtaat gcaacactcc cggtcagttc aaccggtgcg cgatcttcct
    24301 ctacgacaaa attttcgtcc gcgctggtgg gtgacaacaa gatcactgtt ccaccttgta
    24361 ttaccgcatt ttccagcgta ccggcatgca cttcagcccg accacccaca ttcacggaca
    24421 ggttttgcgt cttaccggaa ctgtagagct gaaggccatc cgtccccaga cgataaatgg
    24481 atccattctc aacaatagtg tcagagtctt caccaccgag attattcatg gtgccaccag
    24541 caagcaacgt atcactggct ttcccagcca gcataaaaac gccgccctga ttaatctcac
    24601 aattgctggc ttccccacct ggatacacca acaattcacc accttcatct accacgaccg
    24661 ctgaggttgt cccattaacc aacaggccgc cctcttgatc gagaatgatt ttttccgcgc
    24721 gatgtccttc cagcacacgc aggttaccgc cattttccag caacaaaccg caggcataac
    24781 ctttatcaac gctgaattca ccttcgggat ggcggccatt aaccgtagcg agcgtagaga
    24841 gactaattgc gcctccctcg gactgaacaa tatctgtggc aatggcaccg tcgctgaccg
    24901 atagcgtgcc attgttaccg tactgtgtgc atttggcgat cccaccggtt gtcactttca
    24961 accagccagc ccccgtgacc acggtatttt cggctcttcc gccagcttca acaatcaact
    25021 cgccttcagt aagttgcatc aagccaatga cctcgccggt cttaacaatt tgcacattcc
    25081 cgtcgctcat caagtttttc gcatcagatc ccatgattta ttcctttgct gcatctgtgt
    25141 gcctttattg ctacctaagt gtaaaggcta cggaggattt atccacgaca gatttgagat
    25201 ggtggcaaac aactctgttt aaactctgat acacgaatta ttgggttgta tcagatgtaa
    25261 atgcgatcct gaataaaaat cgcccttgca aatcaacaaa atatagccaa tttgcttaaa
    25321 agctctgcaa acggtcgaaa tagcgcagaa aattacgttt tgcctcttgc caccttccca
    25381 gtctgccgct aatattcgtc cccgttgtca cctacaacgt tgcgttcata gctcagttgg
    25441 ttagagcacc accttgacat ggtgggggtc gttggttcga gtccaattga acgcaccatc
    25501 ctgcgtccgt agctcagttg gttagagcac caccttgaca tggtgggggt cggtggttcg
    25561 agtccactcg gacgcaccag attttcttaa tctggtcttc tccttttccc ctctgtttct
    25621 tctctgtatc caatacgtta aaagatttac actgtcttcg tatgcgttat cagaaggaga
    25681 atcgctatgg caactttgtt acaacttcat tttgctttta atggcccatt tggcgacgca
    25741 atggctgagc agcttaaacc acttgctgag tcgattaatc aggaacctgg ttttctgtgg
    25801 aaggtatgga cagaaagtga aaagaaccac gaagccggtg ggatctacct tttcactgat
    25861 gaaaaaagcg cccttgccta tctggaaaaa cacactaccc gactgaaaaa tctcggcgtt
    25921 gaggaagttg tcgccaaagt tttcgatgtc aatgagccac tgagtcaaat caatcaggca
    25981 aaactcgcct gacagaattt aatcaagggc ggttagcgcc cttttcatcc ctgtctgaaa
    26041 tttctcaaat tctaaaaatc tcaaccaaac ttatctgata acactaaatt cgaaagaatg
    26101 cgtacaggta agtaacaatg aaaaaaattg ctattgtggg tgccgggcct acggggatct
    26161 acaccttatt ctcgcttcta cagcaacaaa ctccactttc tatttctatc ttcgagcagg
    26221 ctgacgaggc cggtgtcggg atgccataca gtgatgagga aaactcaaaa atgatgctgg
    26281 caaatattgc cagtattgaa ataccgccga tttattgtac gtatctcgaa tggctacaaa
    26341 agcaagaagc cagccatctc cagcgttatg gcgttaaaaa agaaaccttg cacgatcgtc
    26401 agtttttacc gcgaattctg ctgggcgaat atttccgcga tcaattttta cgattagtag
    26461 accaggcacg aaagcaaaaa tttgcagtgg ctgtttatga atcatgccag gttaccgatc
    26521 tgcaaattac aaatgctggc gtcatgctcg ctacaaatca ggatttaccc agcgagacgt
    26581 ttgatttagc ggtgattgcc acgggtcacg tctggcctga tgaagaagaa gcaacccgaa
    26641 cgtattttcc cagcccgtgg tcaggcttga tggaagcaaa ggtcgatgcg tgtaacgtgg
    26701 gtattatggg aacatccttg agcggactgg atgcggcaat ggcagtggca attcagcatg
    26761 gttcgttcat tgaagatgat aaacaacacg tcgtttttca ccgcgataac gcaagtgaaa
    26821 agctaaatat tacgttaatg tcgcgcacgg gtattttacc cgaagccgat ttctattgcc
    26881 ctattcccta cgagccctta cacatcgtca ctgatcaggc attaaatgct gagattcaaa
    26941 aaggcgaata tggccttttg gatcgggtat ttagattgat agtagaggaa atcaagtttg
    27001 ctgatccaga ctggagccaa cgcatagcct tagagagcct gaatgtcgat tcctttgctc
    27061 aagcctggtt tgccgagcgc aaacaacgcg acccatttga ctgggcagaa aaaaatctcc
    27121 aggaagtcga acgcaataaa cgagaaaaac atactgttcc ctggcgttat gtcattctgc
    27181 gcctgcatga agcagtacag gaaattgttc cacatctgaa tgaacacgac cataaacggt
    27241 tcagtaaagg ccttgcccgg gttttcatcg ataattatgc cgcaatccct tcagagtcta
    27301 ttcgtcggct gctggccttt cgtgaagcgg gaatcattca tattctcgct ctcggtgaag
    27361 actacaaaat ggaaattaac gagtcgcgca ccgtcctgaa aacggaagac aacagctact
    27421 cgtttgacgt ttttattgat gcccgcgggc agcgtccgct taaagtgaaa gatattcctt
    27481 tccctggact acgcgaacaa ttacagaaaa caggggatga aatccctgat gttggtgaag
    27541 attatacgtt acagcaaccc gaagatattc gtgggcgcgt agcgttcggc gcgttgccct
    27601 ggttaatgca cgaccagcct ttcgttcagg gacttacggc atgtgcagaa attggtgagg
    27661 cgatggctcg ggcggtcgta aaacctgcca cccgtgcacg taggcgtctt tcgtttgatt
    27721 aaaggtaaag ctatcagcac tcaacaaact gatgctgttc attaagacga tagcggactc
    27781 cggctcgaat attgttttca ccttcaatgg cgacggcgaa acgaactctt tcgccgtcat
    27841 gatatgccag tgcggcgctt cctcctggcc cgagaataat tgagtcgacc acgcccgtgc
    27901 tcactaccgt tgaattttca ccgctggcaa tgatgtgtac attgtcgccg ctgttggcaa
    27961 ttctggcgtt tgcgccaaag ctggcgattt gcaccaggtc accattactg gcgacatgac
    28021 atcgttcacc caatgtacaa acgcgtactc gcattccggt attcgcaatc cgactgctat
    28081 ctccagcgct gcttatccgc gaagaattac cggttacagc aacccgtgcg cgttcaccgg
    28141 agctgccaat gtgactgtta aagccgacac ttccaatctg cacgctataa cccatactcg
    28201 caatttgcac ggcataccca gcggttgcga ttcggacgtt atcctcacaa cagccgatgc
    28261 gagcatgatc gtcgctgcgg gcaaactgac tgcaaaatgc tctctctccg ggatctgtgg
    28321 ccagtttttg catagaggaa acttcctgat gcgcgaagtt ttcatccgcc agccattttt
    28381 tccacgcata ctcaaccaga ctctccgccc agtccgtata tccttcctca atcagtgcat
    28441 gatgaatatc ggcataacta ccgccgcgtg ggaaatagcg caggaaccag cgatacatca
    28501 ccgctccgat acgccattct cgcaggtctg ctcgtgtaat gatcattcgt tagtgccggt
    28561 gatagccatc gaccatgctt ttaaaggttt cgtgtggcgt acgccccgtg gtgcaaagat
    28621 aaagatgacc gaagataaaa aagaggctta taaatgccag agcaaaatgc gcctgcaata
    28681 accagtatct aacgccagga aacacatctc ccacggcttg cggatagaga cacagcagcc
    28741 ccgtcaatag taacaacggc agcaatccat acatgacacc aacataggcg acctgctgta
    28801 agggattaaa tttagactgg gttgttgccg ggaaaggatg ttcttccccc tgcataatgc
    28861 caaacaaata aaatcgcgtt tgttttgccg cccgctccag ccaaccctga cgacgaatgc
    28921 gatagtggtg gccattaccc ccaacggcat tgatcagcac aaagccaagc cagcatgcca
    28981 gtaacaaaaa tccgcaaact tcatgcaccg caaccagact tttaaccgca gttgcgccca
    29041 gcatcgcaaa gtgatttatc agcccacttg ccagcaacaa tacaaagagt aacgcattcg
    29101 accagtgcca taatctgacc gctttgctgt aaagataaat cttctcacca tgatcggtgg
    29161 ctgatttctt cacgccacga gcacgaagca aggcgtgcag ccccagcacc aaccacatcc
    29221 caaccagcaa tactccggca atgatcaacc acaccggcca aaattcggga gtgaactgtg
    29281 gcacatagtt cgctaactgg ctctgaaact gttcagcatg ttgcgacggg ttcatacatt
    29341 ttccttttta atcaaatgtt gaccgaaccg gcgatacaga tgcggtttcc cggcaccagg
    29401 tagctgatat tgatagtatt tattatcctg caaccacgcc tgaatttctg ggctatcttc
    29461 gcgaccaaag atcaatgcgt gttccgggca ggcgctgacg caaatgggcg gaaagccttt
    29521 tgccagtcgg gactcagcgc agaaatcaca tttatctgcc actttagtga cagggttaag
    29581 gtagcggacc tggtacggac aagcgccaat gcaataactg cagccgatgc actgcgattt
    29641 ttctacccgc acgatcccct gctcatcacg ccacgacgcc cctgtcgggc atacgtcgat
    29701 gcacggagca tcttcgcagt gctgacatga ctggcgaaaa aagtgatact gcgtttcgtt
    29761 gtcgttatcg gtgactggaa tgtgtgctat cgataaacgg cttccctgag cggggacgtg
    29821 gttagtttta cggcatgcgc gggcgcaaat attacagcca ttgcaccgtg actcgtcatg
    29881 aatcatggca taccgaactt ccttttcttg cctcgtgttc gctaacagag aagacgcaga
    29941 accagtaaaa aagattactg ttcccatccc cagaacaaat ttgcgtcgag tgaaggacat
    30001 ctgattatcc cctaatgcag tggctgcaac tgtccaccct atccatgaca tctccctgaa
    30061 atttcagggg ttctgaaact gctctacctc gaaccattcg cataagccat aagccttgcc
    30121 ataggtcata aatcgctcgc taaacaagta caacaccgcc gtcgatgact ccacctgccg
    30181 tatatcggca tattcaggtg caacatccat cgcggcgatc gcggcttcaa tctgcgcttc
    30241 ctggaagtaa tatggcgctt gcatcagcat ggcgactttg taaggacgag gatacgtctg
    30301 gcactcaaaa cgaacggcgt gtgcaatggc gcgacaaatg tcctgctcca caacttgcaa
    30361 agacatcgcc gcgtagtttt cactcatggc ctgcgttgag taatagtaat catcctgact
    30421 tcccgtcata caagcgatat ccgcgtagtc tccgttctgc ttaatgcttt ccagcaaaac
    30481 tgacagttcc tcttctgcaa ctgaataggg tggcgagaga aacacggcgc gcgccaccag
    30541 ctgcccagcc acagaatgct ggcgaatgaa ctctgccagg atttcagccg gttctggttc
    30601 ggttaccgta tcaacggggt gacgcatccc ctgcaaccat tgccgcttcg cctcgtcaac
    30661 ttcgctgact ttcgctaatg gcaattcatc ccgatggttc atctcaccca ttccttacgc
    30721 aggcagtaga ttgtgtgctg ccagatcggc ggcaatatct tccagcccca gacgctgcaa
    30781 tgtttcgcgg gttgggcaac caagctgtgg gtcccagccc atttctttgt agaacatcgt
    30841 gagcgaggca tgcatatcat cacgatccat tttgtcagta ccttcagtaa agaccgggat
    30901 ctgcggatcc ttgtcgaata cccaggaaca gataagatcg tgttcattac gcatatcttt
    30961 ggtttgcatc agttttaccg tgtaggcacg atgcaacgta aagatacgct ctgcagctaa
    31021 atctaatttt tcctgagtca tctcttcgcc ggtgatcgct ttgaagaatt tggcttcaag
    31081 cgccagatcg ccccggtaat tacggctttt cagcggcgaa acggtcattg gccagaccca
    31141 gttgcacagg gtgacggcgt tatgcaaaca gacccgcaac agcgaccact ttgcatattt
    31201 aatttttgcg tcattgattg gcgtgtagtt tttggtttca tcgtaagcat cttcagaacc
    31261 aaaaagctct ttcgccactt cacgctgcag tttcaatggc aaacccgaac caataaaatt
    31321 gatatgggta tgcgtcatgc agtcacggtt gaacatacag ttaacaatgg aaccgacctg
    31381 cgctgacgct tcattggcat ggtgaaccgg atagccaaac ggcgaccaga gtttattttt
    31441 tgcgtagccc cagtactctt cacccaaatt ccagcgttct gcgatggcat atgaaccatc
    31501 agccaggtgg ctcagctcac ccacacgatg cgccagacgg tagtaaaaat ctttaatgaa
    31561 gttaacgtca cccgcttcca gttgatccca gcgaatttcc gcatactctt cagctggcag
    31621 aacacgcttg aacacccctt tgctgtaaca ataagtaaag tcgcgatgca gctgcccgta
    31681 gttacaccat aggccgtagt catcgaacaa attcagacca accaggttac caatcacacg
    31741 accatcgtct ttatcttcaa aatctttcgg cccgttcggg aagatggtgg tatggacaaa
    31801 gtttgctaca caagtgttac cacctgtgct gggcacgcca aactctttca cccgaggaat
    31861 attcatttgg gtcatacaac ggatcgggca agagtggcag ccgctcattt ttactgtgta
    31921 tttctctgcc gccggtccta agtcaaaaac ggatttatag gtacgaaagc cgaccgtatt
    31981 ctgattgcct ggcggaattt cacccgtttc aatcggacca ccttcagccg cgccccaaaa
    32041 cagcccttta cgtgctgtcc agcgtgactt gggatctgaa tactctgccc acgattgtgg
    32101 cgtacttggc acgacatggt tgttattcgc accgataagt tcagtcatca tgtaatcgtt
    32161 gagacgcttc atctcctgac gatcggcaat gttgacccct ttcgtccctt caaccgcaat
    32221 cgctttcagg tttttcgaac ccattattgc gccagttccc gcaccgccgc tgtggttacg
    32281 gctattcaac atgccagaga gaggaacaag gttttcccca gcctgaccaa tagccgccac
    32341 acaggtttcc ggactggtca agcgacaaat ttcttccgtc gttgcgcgcg tcccttttcc
    32401 ccataagaaa tctgcttttt ccaggctaac tttgtcatct ttaatcttca gccataccgg
    32461 tgatttcgcc ttcccttcga taataatgac gtcgtatcca gcgaatttca tttgcgccgc
    32521 aaaaaagccc cccatatggg catcgacgac taaatttcct ttggtaaaag tagaaagtga
    32581 ggtgatattt acgcgagaac tacagggggc accagatcca gttaatgggc cggtagcaaa
    32641 gactaattta ttcgcttcat cgaaaggttt cgtgcctggc ggtacttcgt catacataat
    32701 tttgtagccg aagcccatgc caccgacaaa acttttaaac ttactggaat cttcgagggt
    32761 aatatttcct gtcgtgagat tgactcttaa tatattacct gtccaaccgt tagccatgat
    32821 tttttccttt gcaagattca cacagtaata tctttccact cgataatttt taacgcccct
    32881 gttgggcagg cgtttgcgca ttcaccgcat aacacacatt tcgaggattt tttactttcg
    32941 gtatttacgg tggccatcat ccacgggcat gccgtagtac aggcgctaca gccaatacaa
    33001 cgtttatgat cgacggtaat acagccttct ttctgctgcc aggtaatcgc accaatcggg
    33061 cagacgttca tgcattgcgg ctctttgcat tgacggcagg tgtccgcggt atagttgaga
    33121 tcgccataca ggccgccgcc agagccaacc ccgttgtcgc caaagaaata attgcggtgg
    33181 attttgatac gggagaagaa tgtccctact gagccatcgt tgaagttggt acaagagatt
    33241 tcacagcggt ggcaacccgt gcatcgcgct cgctgggtca ccagcacccc tttgggtgta
    33301 tttatcaacc ctactgtgcc gctatcaata tcttcctgtt tgcaacccaa aagtgacagt
    33361 aacgcagggg caattgttag accagcaagg ccttttcctg atatacgcaa gaattctaat
    33421 cgcgttaagc caatatctaa tagtggacga tcaactgggt tcatttattt cgcatcctct
    33481 tcgcaaacac agtgctgaca attcagcagc ctgatattaa gcattcaact aatacgctgc
    33541 tgactctgtc cccaacacca gatattttcc ctaacctgat agtgtatgag aaaagattta
    33601 acgccgataa accaatcctt tatggttagg agaaa
//
LOCUS       DAZUCE010000022        33395 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZUCE010000022 DAZUCE010000000
VERSION     DAZUCE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.022"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(48..1232)
                     /locus_tag="ACHY03_000983"
     CDS             complement(48..1232)
                     /locus_tag="ACHY03_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3904247.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(1532..1626)
                     /locus_tag="ACHY03_000984"
     tRNA            complement(1532..1626)
                     /locus_tag="ACHY03_000984"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1590..1592),aa:Sec,seq:tca)
     gene            1919..3301
                     /gene="yicJ"
                     /locus_tag="ACHY03_000985"
     CDS             1919..3301
                     /gene="yicJ"
                     /locus_tag="ACHY03_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFS3904248.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNEPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3311..5629
                     /gene="yicI"
                     /locus_tag="ACHY03_000986"
     CDS             3311..5629
                     /gene="yicI"
                     /locus_tag="ACHY03_000986"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFS3904249.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAV
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACTWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARIGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(5682..7391)
                     /gene="yicH"
                     /locus_tag="ACHY03_000987"
     CDS             complement(5682..7391)
                     /gene="yicH"
                     /locus_tag="ACHY03_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFS3904250.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYSLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTGQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PAQ"
     gene            complement(7512..8903)
                     /gene="xanP"
                     /locus_tag="ACHY03_000988"
     CDS             complement(7512..8903)
                     /gene="xanP"
                     /locus_tag="ACHY03_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFS3904251.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9183..10388
                     /gene="gltS"
                     /locus_tag="ACHY03_000989"
     CDS             9183..10388
                     /gene="gltS"
                     /locus_tag="ACHY03_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFS3904252.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(10564..12645)
                     /gene="recG"
                     /locus_tag="ACHY03_000990"
     CDS             complement(10564..12645)
                     /gene="recG"
                     /locus_tag="ACHY03_000990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFS3904253.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLQTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNITFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(12651..13340)
                     /gene="trmH"
                     /locus_tag="ACHY03_000991"
     CDS             complement(12651..13340)
                     /gene="trmH"
                     /locus_tag="ACHY03_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFS3904254.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(13347..15455)
                     /gene="spoT"
                     /locus_tag="ACHY03_000992"
     CDS             complement(13347..15455)
                     /gene="spoT"
                     /locus_tag="ACHY03_000992"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFS3904255.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(15474..15749)
                     /gene="rpoZ"
                     /locus_tag="ACHY03_000993"
     CDS             complement(15474..15749)
                     /gene="rpoZ"
                     /locus_tag="ACHY03_000993"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFS3904256.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(15804..16427)
                     /gene="gmk"
                     /locus_tag="ACHY03_000994"
     CDS             complement(15804..16427)
                     /gene="gmk"
                     /locus_tag="ACHY03_000994"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFS3904257.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            16685..18367
                     /gene="ligB"
                     /locus_tag="ACHY03_000995"
     CDS             16685..18367
                     /gene="ligB"
                     /locus_tag="ACHY03_000995"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFS3904258.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWRKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDEQS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(18364..18981)
                     /gene="yicG"
                     /locus_tag="ACHY03_000996"
     CDS             complement(18364..18981)
                     /gene="yicG"
                     /locus_tag="ACHY03_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFS3904259.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19272..20096)
                     /gene="dinD"
                     /locus_tag="ACHY03_000997"
     CDS             complement(19272..20096)
                     /gene="dinD"
                     /locus_tag="ACHY03_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFS3904260.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQISIKQLENSVKITEKK"
     gene            complement(20318..21181)
                     /gene="yicC"
                     /locus_tag="ACHY03_000998"
     CDS             complement(20318..21181)
                     /gene="yicC"
                     /locus_tag="ACHY03_000998"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFS3904261.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKALIEQRL
                     EGVTAEVVKVRSHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21308..22024
                     /gene="rph"
                     /locus_tag="ACHY03_000999"
     CDS             21308..22024
                     /gene="rph"
                     /locus_tag="ACHY03_000999"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFS3904262.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22090..22731
                     /gene="pyrE"
                     /locus_tag="ACHY03_001000"
     CDS             22090..22731
                     /gene="pyrE"
                     /locus_tag="ACHY03_001000"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFS3904263.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22768..23364)
                     /gene="slmA"
                     /locus_tag="ACHY03_001001"
     CDS             complement(22768..23364)
                     /gene="slmA"
                     /locus_tag="ACHY03_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFS3904264.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(23471..23929)
                     /gene="dut"
                     /locus_tag="ACHY03_001002"
     CDS             complement(23471..23929)
                     /gene="dut"
                     /locus_tag="ACHY03_001002"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFS3904265.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTALVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(23907..25127)
                     /gene="coaBC"
                     /locus_tag="ACHY03_001003"
     CDS             complement(23907..25127)
                     /gene="coaBC"
                     /locus_tag="ACHY03_001003"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFS3904266.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILTPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25299..25967
                     /gene="radC"
                     /locus_tag="ACHY03_001004"
     CDS             25299..25967
                     /gene="radC"
                     /locus_tag="ACHY03_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS3904267.1"
                     /translation="MKNNAQLLMPREKMLKFGISTLTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26184..26420
                     /gene="rpmB"
                     /locus_tag="ACHY03_001005"
     CDS             26184..26420
                     /gene="rpmB"
                     /locus_tag="ACHY03_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFS3904268.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            26441..26608
                     /gene="rpmG"
                     /locus_tag="ACHY03_001006"
     CDS             26441..26608
                     /gene="rpmG"
                     /locus_tag="ACHY03_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFS3904269.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            26706..27515
                     /gene="mutM"
                     /locus_tag="ACHY03_001007"
     CDS             26706..27515
                     /gene="mutM"
                     /locus_tag="ACHY03_001007"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFS3904270.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(27554..28033)
                     /gene="coaD"
                     /locus_tag="ACHY03_001008"
     CDS             complement(27554..28033)
                     /gene="coaD"
                     /locus_tag="ACHY03_001008"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFS3904271.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28041..29318)
                     /gene="waaA"
                     /locus_tag="ACHY03_001009"
     CDS             complement(28041..29318)
                     /gene="waaA"
                     /locus_tag="ACHY03_001009"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFS3904272.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            29731..30789
                     /gene="rfaQ"
                     /locus_tag="ACHY03_001010"
     CDS             29731..30789
                     /gene="rfaQ"
                     /locus_tag="ACHY03_001010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFS3904273.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            30786..31910
                     /gene="waaG"
                     /locus_tag="ACHY03_001011"
     CDS             30786..31910
                     /gene="waaG"
                     /locus_tag="ACHY03_001011"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFS3904274.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVEANCGEAIVEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            31903..32700
                     /gene="rfaP"
                     /locus_tag="ACHY03_001012"
     CDS             31903..32700
                     /gene="rfaP"
                     /locus_tag="ACHY03_001012"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFS3904275.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQQDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            32716..>33395
                     /locus_tag="ACHY03_001013"
     CDS             32716..>33395
                     /locus_tag="ACHY03_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS3904276.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKTLITMDLANNVAAVVTER
                     DANWWSLRAQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNL"
BASE COUNT         7945 a         8269 c         8834 g         8347 t
ORIGIN      
        1 gataaaatac ttaattgagg aaaacatcag ggaaaagtat ttctgactta actttcccgg
       61 ctggagagtg aatcaatgta atcagcgtac cattgcatca tttcacggcg gccatcgaga
      121 tacagggcat gattataggt acccctgatg gaatttttat ccacatgagc taactgcatt
      181 tcaatccagg cggaattaaa gccttgttca tgcagaattg tactcatggt gtgtctgaaa
      241 ccgtgccctg tgagtcttcc cttatatcca agtaattcaa ttacttggtt aatgctttcc
      301 ttactaattg gtttgcgggg atcatttcta ccgataaaga ctaggggatg atgttttgag
      361 attggttcaa gctgcttaaa tagcataatg acctgctcag agagcggcac aatatgaggt
      421 cgcttcatct tcattacttc tgccgggatc tcccacaatt ttgtctcaaa atcgatatct
      481 tcccagcgtg caaaacgcag ttcttgtgtt cgtacgccgg ttaacataat gatctgagta
      541 gctgtcttag tgattatact tccggtataa cccgccagat cgttgaggaa gtggggaagt
      601 tcattagcag taagaaaggg aaaatgtact tttttcggtg tagctaaagc actggcaaga
      661 tcgggggctg ggttatagtc tgcccggcca gtaacaattg catagcggaa cacctcacca
      721 cagcgctggc ggaccttgcg cattttctcc agagctccac gtttttccat tttacgcagt
      781 gcttccagta actccattgg tttgatttcg gcaatagggc gcttgccaat gtagggaaaa
      841 atgtcttttt caaaggtatc aatgatttca tcacgatagc gtaatgacca ccgatcggct
      901 tttgactggt gccattcgcg agcaatagcc tcgaacgtat ttttcagact catttgctgc
      961 gcgatttttt cttccttttt tgcctcccct ggatcgcctc cagcagcgag gagtttcctg
     1021 gcttcactgc gtttctcgcg agcatcggct agcgttacat cggggtatac gccaatagaa
     1081 agcatctttt ctttaccagc aaagcggtac ttcatgcgcc agtatttaga accattagtg
     1141 ttaatcagta gatacatacc gccgccatca gcaagcttat agggtttgtc tttgggtttt
     1201 gcggttttca ctttgatatc ggttaatgcc atgacatcct ccggcttttg atgaattcat
     1261 gttcctgagg gggatttctg ccagtaacta acctgatacc cccaatacac cccttgtata
     1321 acaaagatgt tgttaaaagc cgttgttata tatcacattt ttcctattat gggggtgctg
     1381 gtatttcaat aggaaataac accccctaaa gtacccccaa aagaaacttg atacggggtg
     1441 aagtaggttg acctctggat acaaataaat actgatatac aagggattag aagagattta
     1501 ttgacattgg gagagttcag aagaggtaaa ctggcggaag atcacaggag tcgaacctgc
     1561 ccgggaccgc tggcggcccc aactggattt gaagtccagc cgcctcaccg gagacgacga
     1621 tcttccgcgc ctcgattgct acatggaggc ggggcgcatt atagctactt ccttgagttt
     1681 ctacatcccc cagatcagtt ttttccgcct aatacacgca gcgtattatc acgaaagcat
     1741 tatagttcat ataaaaccca tattttacaa tgatatatga gcggaattaa cgctaccacg
     1801 atcacataaa acaagaatca taaattaata accagatatc ggaatattcg ctctcacagg
     1861 gatggtttac aaaatgcgtt atcggtctca gcacccctat agcatcaagg aaaagcagat
     1921 gaagagtgaa gtgttgtccg ttaaagagaa aattggttat ggcatgggag acgccgccag
     1981 ccacattatt ttcgataacg taatgttata tatgatgttc ttttataccg atatttttgg
     2041 cattcctgcc ggatttgtcg gaaccatgtt tttggtcgct cgtgcactgg atgcgatttc
     2101 cgatccttgc atggggttgt tggccgatcg aacgcgctct cgctggggta aatttcgtcc
     2161 gtgggtactg tttggcgcac tgccattcgg gatcgtctgt gtactggcct atagcacgcc
     2221 agatctcagt atgaacggca aaatgatcta tgcagcaatt acttacaccc tacttacctt
     2281 actttatacc gtcgtcaata tcccttactg cgcattgggt ggtgtaatca ccaatgaacc
     2341 gactcagcgt atctcgctgc aatcctggcg ttttgtgctg gcgaccgcgg gaggcatgct
     2401 ctctactgtt ctgatgatgc cactggttaa tttaattggt ggtgataata aaccactcgg
     2461 tttccagggc ggtatcgcgg tcctttccgt ggtggcattc atgatgctgg cattctgttt
     2521 cttcaccact aaagaacgcg ttgaagcacc acctacaacg acgtctatgc gggaagattt
     2581 acgtgatatc tggcaaaacg accagtggcg gattgtcggt ttactaacca ttttcaatat
     2641 cctggcggtg tgcgtacgcg gtggggcgat gatgtattac gtcacatgga ttttgggtac
     2701 gccggaagtg tttgtcgctt ttctcaccac ttattgcgtg ggtaacctga ttggttccgc
     2761 tctggcaaaa ccgctgaccg actggaaatg caaagtcact atcttctggt ggacgaacgc
     2821 cctgctggca gtgattagcc tcgcgatgtt ctttgttccc atgcaggcca gcatcactat
     2881 gtttgtcttc atcttcgtga ttggtgtgtt gcatcaactg gtgacaccta tccagtgggt
     2941 aatgatgtcc gataccgtcg actacggcga gtggtgcaat ggtaaacgcc tgaccgggat
     3001 cagttttgct ggcacgctgt ttgtgctcaa actggggttg gccttcggcg gcgctcttat
     3061 cggctggatg ctggcttatg gcggatatga tgcggcagaa aaagcgcaga acagcgccac
     3121 gattagcatc attatcgcgc tattcacgat tgttccggcg atctgttatt tgctgagcgc
     3181 gattatcgct aaacgctact actcactcac gacgcacaat ctgaaaaccg ttatggaaca
     3241 gctggctcag ggcaaacgcc gttgccagca acaattcacc tctcaagaag tgcagaacta
     3301 aggaacggca atgaaaatta gcgatggaaa ctggttgatt caacctggcc tcaatttgat
     3361 tcacccgctt caggtgttcg aggttgaaca gcagggtaat gaaatggtgg tctatgctgc
     3421 cccccgtgat gtacgtgaac gtacctggca gcttgacacg cctttattta cgctgcgctt
     3481 tttctcccca caggaaggta ttgtcggggt gcggattgag cattttcagg gggcgctgaa
     3541 taacggccct cattatccgc tcaatatttt gcaggacgtg aaggtcacaa tcgaaaacac
     3601 agaacgttat gctgtgttta aaagtggcaa cttaagcgcg cgtgtcagca aaggtgagtt
     3661 ctggtcactc gattttctgc gcaacggtga acgtattacc ggtagtcagg tgaaaaataa
     3721 tggctacgtg caggacacga ataatcaacg aaattatatg tttgagcggc ttgatcttgg
     3781 cgttggcgaa acagtttatg gtctgggaga gcgctttact gccctggtgc gcaatggtca
     3841 gacggtagaa acctggaacc gggacggcgg cacaagtact gaacaggcgt ataaaaatat
     3901 cccgttctat atgactaacc gtggctatgg ggtgctggtc aatcaccccc agtgtgtctc
     3961 ttttgaagta ggatcggaga aagtctccaa agtgcagttc agcgttgaga gcgaatatct
     4021 cgaatacttt gttatcgacg gtccgacgcc gaaagcggta cttgatcgtt atacccgttt
     4081 tactggtcgt ccggcgctgc cgcccgcgtg gtccttcggt ctgtggctaa ccacttcatt
     4141 taccaccaac tacgacgaag cgacggtaaa tagctttatt gatggtatgg cggaacgcaa
     4201 tctgccgctg catgttttcc actttgactg tttctggatg aaagccttcc agtggtgcga
     4261 ttttgagtgg gacccgctga ctttcccgga cccggaaggg atgatccgcc gcctgaaagc
     4321 gaaagggctg aaaatctgcg tctggattaa cccctatatt gggcaaaaat cccccgtctt
     4381 taaagagtta caagagaaag gttatttact caaacgcccg gacggttcgc tgtggcagtg
     4441 ggataaatgg cagccaggtc tggcgattta tgactttacc aatccggatg cctgcacatg
     4501 gtacgccgac aaactgaaag gtctggtcgc gatgggcgtt gattgcttta agaccgactt
     4561 tggcgaacgt atcccaaccg atgttcagtg gtttgacggt tccgatccgc agaaaatgca
     4621 taaccattat gcgtacatct acaacgaact ggtgtggaac gtgctcaagg acaccgttgg
     4681 tgaggaagaa gccgtcttgt ttgcccgctc ggcctccgtc ggtgcgcaga aattcccggt
     4741 acactggggt ggcgactgtt acgctaacta cgaatcaatg gcggaaagcc tgcgcggtgg
     4801 tttgtctatt ggcctttcag gttttggctt ctggagccac gatatcggcg gctttgagaa
     4861 taccgcgccg gcgcacgttt acaaacgctg gtgcgcgttt ggtttgctct ccagccatag
     4921 tcgtttacat ggcagcaaat cttatcgtgt gccgtgggcc tacgatgatg agtcctgtga
     4981 tgtggtgcgc ttcttcacgc aactgaaatg ccgcatgatg ccgtatctgt atcgtgaagc
     5041 tgcacgtgca aacgcccggg gtacgccgat gatgcgggcc atgatgatgg agttcccgga
     5101 cgatccggct tgtgattacc ttgaccgtca atacatgtta ggcgacaacg tgatggttgc
     5161 gcctgtgttc actgaagcgg gcgatgtgca gttctacctg ccggaaggtc gttggacaca
     5221 cctgtggcac aacgatgaac tcgacggtag tcgctggcat aaacagcagc acagcttcct
     5281 gagtctgccc gtttatgtgc gtgatagcac cctactggcg ctgggcaaca acgatcaacg
     5341 tcccgattat gcgtggcacg aaggtacagc attccacctc tttaatctgc aagacgggca
     5401 tgaagccgtc tgtgaagtgc ccactgctga cggttcggtg atctttactt taaaagcagc
     5461 acgtattggc aacacgatta ctgtgactgg tgcgggcgag gcgaagaact ggacgctgtg
     5521 cttgcgcaat gttgtgaaag taaatggtct gcaaggcggt tcgcaggctg aaagtgagca
     5581 ggggctggtg gtgacgcctc aagggaatgc gctgacaatt acgttgtaat ctattgatgg
     5641 aatgccgggt acgtctgagt cgtacccggc gagattgatg tttactgcgc cggctccgtc
     5701 gacgtaactt caccactgga aactacgccc gcattcatgg tttgagtcac ttgctgcttt
     5761 gcggcattca cggcatgcaa ttgcccgcta accgtaggtt tcagcgggac attggcctga
     5821 atatcgccac tggcggtaag ctgaatattg ccgtcgccag tcaacggtaa ttcaggccat
     5881 ccccaatgtt gcaaaatatt caccggcaca ccgcgtccat tcaggctgat atgggtctga
     5941 cgttgtggcg tttgtgaaac actggcggtg gcttccagaa tgcctttttc agtaaatgca
     6001 ctcagttcgc tgatattcac cgtgctgctg ttggcggtta gcgccagcga tggacgacgg
     6061 acatcaacac gattaaacgt cgcggcggcg gcattcagat tcgccgagcc actccagacg
     6121 ccccatttat gatcggtaac cagcgtcaga ttggcaccgt aaccatcgag cgcggtgagc
     6181 tgccacggga agtcagggtc gatatcaatg atcagattgc ggctggcgct aaatctcttc
     6241 agttgcaggc tgtttaacca accgggtgtc gtttccatcc acaactgttg ccagtttttc
     6301 ggcaaggtat attccagccc ggcaattgcc gcatcatcaa ggatcaacgt tttcccgtca
     6361 cgcagccagt tccctgacgt tctgaccata cccccttccc agcggctggt gaactggcgc
     6421 agcgctacgc cctgcgggga aaattccgcg tttataatcg ggtcaaataa atgcagcgaa
     6481 ccataaatga actcgctggc gttcatcgac agtttgcctt cctgtgtctg ccagtcatct
     6541 ttactgaagg tcatgttgcg caagctgaga tcgaggtcgg tcaccgccca gtccggacct
     6601 tgcaaacgag catcgatcac ttccaggcga ccaatctgca acgacgggac agagcgtaat
     6661 ggcgcaaaga agtcggttag cgatttttcg ctttgtagac ggatatccgc catgcgcaga
     6721 ttttccactt gccagctgcc gtcggcgtta cgctgcgcgt ttccggttaa tgtcccgcgg
     6781 gcgatgtcgg cacccaggtt agtcagcgta acgcgatcgt tatcaatact gccttcaatc
     6841 agtacattgg tggcaggaac atcattgagc gaaagcgatc cggcactaaa ctgaatctgc
     6901 gccttcgtac ccagcacttt accggcttct ggtgaccacg gaaccacgcc gccatttacc
     6961 cgctgcgcgc tcagtttcca ttcgctattc gggctattaa acgccatatc acgcagttgc
     7021 agacgatcgg ctttgaacgg tagcggcgcg gtctggccgg tgagattcag cgtcccgttt
     7081 tccagcagga tggtatcgac atggcgtggt tcggtcagtt gccgactgct tagcgcaatg
     7141 tcgacacttt ttgccaccag ggtcgcgggc tgaccatcac gaccaaatgt gacgttctcc
     7201 agcacgatat gagatggcgc ggaaaaacgg tgatccatcg ccccgaaggc cagatgatag
     7261 tcgctattct cggaaaccca tgcgctgata tgttctgctc cccagcgggt ttgcagaaga
     7321 gaatagaggc cagcgatcac cactaacaga gcgatgagaa tgtagagaag cagcttccca
     7381 ataaatttca tggtcttcca tcccgtgaaa tgcacataag ggagttatgc acgatttacg
     7441 cgcaatcctc aaggcgggaa tggtgaaaga ggtgagactg cggcggtaat tatcttaccg
     7501 ccgccagtga attactgttt ttctggtggg aaaatcagat tcagcacgat ggcagtaata
     7561 ccgcccgcgg cgatcccgga ggagagcagg tttttcagcc attcaggggc aaactgcaaa
     7621 atcagcggct gctgagacac gcccaggccg accgccagcg acagcgcgat aatcagaatc
     7681 gcccgacggt tcagcggctc acgagaaacg atacgcacac cggaggcggc gatggtgcca
     7741 aacattacaa gcgttgcgcc gcccagaacc ggttctggaa tgtgttgtac aaaaccgctc
     7801 actgccggga acagacccag cacgatcaac atcagcgcga cgacaaaacc gacatagcgg
     7861 ctggcaacac cggtcaactg gatcactccg ttgttctgcc cgaagcagga gttcgggaag
     7921 gtgttaaaca ccgccgaaac aaacgagttc aggccgtttg ccagcacgcc gcctttcagg
     7981 cgtttcatgt acagcggacc ggacactggc tgttcggaaa cgtcagaggt cgccgtgata
     8041 tcgccaatgg tttccagcga agtgatcata aagaccagca tcagcggcag cagcagactc
     8101 cattcaatgc caagaccgta atagagcggc gttggcacca taatcagttc ttgcgtcatc
     8161 ggttcgttgc tttctggcaa catgcccata aaccacgcca gcgcatatcc ggccgccatc
     8221 gcaatcacca gtgaggccac gcgtaagtaa gggttacgtt gacggttaag caggataatt
     8281 aaggctaaga ccacgcctgc cagcagcaga tttttcggtg cgccgaaggt gttatcgctc
     8341 atggctgcgt aaccgccgcc aattgaggtc agccccacct gaattagcga caggccgata
     8401 atcatcacca caacgccaga aaccagcggc gtaataatgc ggcgcgccag atgcagaaca
     8461 cgggagatca ccatctcggt gcaacttgcc agcatcaacg tgccgaacaa agccgccatc
     8521 atggtaggaa catcagcacc accggttttc agcgcggtac cgcccataat cagcggggca
     8581 acaaagttga agctggtgcc ctgaatagac aacagcccgg agccaaccgg accccaggcc
     8641 ttaatttgaa taatcgatgc cacaccggag gcaaacagcg acatactaat aatgtgttgc
     8701 gtgtcttgtg ccggtaaacc cagcgcctgg cagattaata gcgctggcgt gatcaccgca
     8761 acgaacatcg ccagcaaatg ctgacaggcg gcaaacaggg tttgaggaag cggcggacga
     8821 tcttcaagac ggtaaatcag ttcgctgttt tgagtctgcg caaccggttg cgcattttct
     8881 gactcgaggg tggaaacaga catcgaaaac aatcccgtgg tagcaaagcg ggcattttag
     8941 ctgaccgaaa gagaaaagca aacgtttgcc acatcctcat ctaaatattt aaatgagata
     9001 aatgcacttt tttatataac ttttggtatt tttctgctta aaatccatgc catttgttca
     9061 ttaacagctg agaatttgac ccaggaaagc ataactcgat acccgtcata gttcatgaag
     9121 ttgccactgc gcgatgaagt atgacgagta tgaaagagtg atgcggatac aaaggagtaa
     9181 ctatgtttca tctcgatact ttagcaacgc ttgttgccgc aacgctgacg ttgctgctcg
     9241 ggcgtaagtt ggtccattcc gtctcctttt tgaagaaata caccataccg gaacctgttg
     9301 cgggtggttt gttggtggcg ctggcgctac tggtactgaa aaaaagcatg ggctgggaag
     9361 tcaactttga tatgtccctg cgcgatccgt taatgctggc attcttcgcc accattggcc
     9421 tgaacgccaa cattgccagt ttgcgtgccg gtgggcgtgt ggttggcatc ttcctgattg
     9481 tggttgttgg tctgttggtg atgcaaaatg ccattggcat tggtatggct agcctgttag
     9541 ggcttgatcc gctgatgggg ctgttggccg gttctattac gctttccggc ggtcacggta
     9601 cgggagctgc atggagtaaa ttgttcattg aacgttatgg cttcaccaat gcgacggaag
     9661 tggcgatggc ctgtgcaacg ttcggtttgg tgctgggcgg cttgattggc ggtccggtgg
     9721 cgcgctatct ggtgaaacac tccaccacgc cgaacggtat tccggatgac caggaagtcc
     9781 cgacggcgtt tgaaaagccg gatgtgggcc gcatgatcac ctcgctggtg ctgattgaaa
     9841 ctatcgcgct gattgctatc tgcctgacgg tggggaaaat tgttgcgcaa cttttggctg
     9901 gcactgcttt tgaactgccg accttcgtct gtgtcctgtt tgttggcgtg attctgagca
     9961 acggtctgtc aatgatgggc ttttaccgcg tctttgagcg tgcggtatcc gtgctgggta
    10021 acgtaagcct gtcgttgttc ctggcgatgg cgttgatggg gctgaaactg tgggagctgg
    10081 cttcgctggc gctgccgatg ctggcgattc tggtggtaca gaccatcttc atggcgttgt
    10141 atgccatctt cgttacctgg cgcatgatgg gcaaaaacta cgatgcggca gtgctggctg
    10201 cgggtcactg tggttttggc ctcggtgcaa cgccaacggc aatcgccaac atgcaggcga
    10261 tcactgaacg ttttggcccg tcgcacatgg cgtttctggt ggtgccgatg gtcggtgcgt
    10321 tctttattga tatcgttaac gcgctggtga ttaagctgta tttgatgttg ccgatttttg
    10381 cgggttaacc gatgaagcgg cggtagaatt gccgccgcaa caaagacaaa tgcctgatac
    10441 gcttcgctta tcaaggctgc gcattgattc acaatttatt gaatttataa gtttttgtag
    10501 gacgaagcac atccggcagg aaggtagggt aacctgaaat ggcggtcttc tcaccgccgc
    10561 cttttacgca ttcgagtaac gttccgtctc cggcatccag cgttctatca gggcttttgc
    10621 ctgttgtggg taacgttcgt gaatatggcg tgccaggcgc tgaacttccg ggatcatcgc
    10681 ctgatcgcgc agtaaatccg ccactttaaa ttcagcatta cccgtctgac gcgtacctaa
    10741 cagttcaccc ggtccgcgaa tctctaagtc tttctgtgca atcacaaaac cgtcgttgct
    10801 gtcgcgcagc acttgcaggc gaatttgcgc ggttttggag agcggcgttt tgtagagcag
    10861 cacgcagtga gaagccaccg caccacgacc tacacgcccg cgcagctggt gtaactgcgc
    10921 cagacccaga cgctcagggt tttcgataat catcagactg gcgttaggca catcaacacc
    10981 gacttcaata acggtagtgg caaccagcag gtgtagttca ccttgtttaa acgacgccat
    11041 caccgcctgt ttctcggcag gtttcatccg cccgtgtacc aggccaacat tcaactctgg
    11101 tagcgccagt ttcaactctt cccaggtagc ttccgccgcc tgcgcttcca gcaattccga
    11161 ctcttcaatc aacgtacaaa cccagtacgc ctgacgacct tcagtcatgc aggcgtggcg
    11221 cacgcggtca atgatgtcgg tacggcgagt atcaggaata gcgaccgtag tcactggcgt
    11281 gcggcctggc ggcagttcat caatgaccga ggtgtcgaga tcggcatacg cagtcatcgc
    11341 cagcgtgcgg gggatcggcg tggcggtcat gatcaactga tgcggatgga agccttgctg
    11401 ctggcctttc tcccacaatg ccagacgctg atgcacgcca aagcgatgct gttcgtcgat
    11461 aatcaccagc gccaggccgt taaactgcac ctgttcctgg aagatggcgt gtgttccgac
    11521 aatcatctgc acctgaccgc tggcgatggc ttcctgctgt gccagccgcg ctttaccttt
    11581 ctgcttaccg gcgagccagc ccacttcgat accgagcggt gcaaaccagt tacggaagtt
    11641 attggcgtgc tgttcggcga gtaattcggt tggtgccatc aatgccacct gtttgccgtg
    11701 ggcaatcgca cgcagcgcgg cgagggcagc gaccagcgtt ttaccggaac ctacatcgcc
    11761 ctgtaccagg cgcatcatcg gcacatccag cgccatatcg cgctcgatct ccgccaccac
    11821 gcgagcctga gcgcccgttg gcttgaacgg taaggcggca agaagtttat ttttcagcgt
    11881 gtcattggcg ctcagcggct gggcatggaa acgttgcgct ccggcgcgta aggctaacat
    11941 gctgaggtta tgcgccagca gttcttccag aatcagacga cgttgcgccg gatgctgccc
    12001 ggtttccaga tcgctaagtt gcagcgtcgg cggcgggcgg tgcaaagtgc gcaacgcttc
    12061 cggtagcgtc atcattcctt gtgacagttc cggcggcagg agttcttcaa tggcgcaggt
    12121 gtcgagcaga tccagcgcct ggtcagttaa tttacgcagc gtggcctgct ttacgccttc
    12181 ggttgttgga taaaccggcg tgagcgtttc ctgcaactct ggcgtactga gatcgccctg
    12241 cacgcggtat tccgggtgga tcatctccgc accatattta ccgcgctttg cttctccata
    12301 ggccagcaca cggcggcccg tcgccaggct atttttcatt gccgcgttga agttgaaaaa
    12361 gcgcatggtg agaatgccgg aaccgtcgct gatctggcag gtcatcatcc ggcggccgcc
    12421 gaaggtgata ttgcaattca gcacttcgcc ttccaccgtg gcataaacgc ccggtagtag
    12481 ttctccgatg gggtagagat gggtgcgatc ttcgtagcgc aggggaaggt gtaagagtag
    12541 atcctgcaca gtctgcaggt tgattttcgc cagcttgtta ctaagcgctg cgccaacgcc
    12601 cgttagggaa ctgagcggga cagcatctaa caggcgacct ttcatggcac ttaccctgca
    12661 gcctgcatag tagcccacca gtcggcatca gcttcgatct cgccttgctg attgacgtgg
    12721 gggtaaggca ggcctttgcg ttttgcgact ttcgccagca ccggatagcc gccttcaaac
    12781 aacaggcgtt gttgctctgc ttccggcaac atgctgtttt cacgcaggta catgcccgca
    12841 ttttgccgct ggcgctgggc ttcgtaaaga atgagggctg aggcaacgga aacattcagc
    12901 gattgcacca tgccaatcat cggaatgatg atgtcccgat ccgccagggc caacgcttcc
    12961 tcagtgatgc ccgttttctc ctgacccatc aaaatgcagg tcgggcgagt gtaatcaatt
    13021 tcgcgaaaat cgacagcgtt atcagaaaga tgggttgcca gaatttgcat gccctggcct
    13081 ttgagatgag cgacggcatc gccaatggtg cggtgcgttt tcacctgcac ccaactgttg
    13141 ctacccgccg ccgcagaggc catggtacgc atgcggctac caggccagac ggcgtgaact
    13201 tcatgtacgc caacggcatc tgcggtacga ataatcgcag aaacgttatg aggtttgtgg
    13261 acctgctcca tgcagacggt cagatcaggc tgccgcctgg cgagcatttc gcagatgcgt
    13321 gcataacgtg ttgggttcat aaaacattaa tttcggtttc gggtgacttt aatcacgtct
    13381 ggcatcacgc ggattttgcg catgatattc gccagatgca cacggtcacg agcggtcaga
    13441 cgaataaagg cgctgtagac gcgaccatct ttctcttccg tattcaaact ttgaatattc
    13501 gaagtcgtgg tgttaattgc cgccgtcagg tttgccagcg cgccctgatg attgaacatc
    13561 tccaccttga tttcggtgat gaactcctgc gccgtctctt tatcccattc cacagccata
    13621 aacttctctg gctctttctg gtagccacgg atgttacggc aggattcatg gtggatcacc
    13681 agacctttac cggggctgac gtgggcgata atcgggtcgc caggaatagg gcggcagcat
    13741 ttcgcaaagg tgatcagcac gccatcggca cctttaatgg gcagatgtcc gtgactttgg
    13801 gttgccggtg gaatggaggc gtccccatgt tgcagatttt tcgcgaccac cacgctcatt
    13861 gcgttaccaa gtccgatttc tgccagcaga tcgtcaagcg ttgccagctt catacgatcc
    13921 agctcgcgct gaatattttc ctgcgggatt tcattcagct tacggctacc acccaaagca
    13981 tggttgagca gacgacggcc caggcttaca gaatcatcac gcttgaggtt tttcagcaac
    14041 tgacgaattt tggcgcgcgc tttcgagcta acgacaaagt tcagccacgc ggcattcggg
    14101 cgagcgcccg gtgcggtaat gatttcaacg gtttgaccgc tggtaagcgg ctgcgacagc
    14161 gggtaaggct gacggtcgac gcgtgcgccc acgcaggcat gaccgatatc ggtatgcact
    14221 gcataagcga agtcgacagg cgttgcaccg gcaggcagtt cgacaatgcg cccttccggt
    14281 gtgaaaacgt aaatctcatc cgggaagaga tcggatttaa cgctctcgat aaattcaaac
    14341 gagctaccgg cgctttgttg cagctccagc aggctttgca tccagcgctg ggcgcggatt
    14401 tgtgcagtcg tgctggtttc gccgtgctct ttataagccc agtgcgcggc aacacccatc
    14461 tccgccatct gatccatatc ttcggtacgg atttggacct caaccggcac gccgtgcggg
    14521 ccaatcatcg aggtgtgcaa cgactgatag ccgttcgctt ttggaatggc gatgtagtct
    14581 ttcacgcgac ccggacgcgg cttatacagg ctatgcatct ggccgagcac gcgataacag
    14641 gtgtcggaat cattgacgat cacgcggaaa gcgtagatgt ccatgatcga gtgaaaacgc
    14701 tgctctttga gcaccatttt gcagtaaatc gaataaagat gcttctcgcg accactgacg
    14761 cggcacggta ttcccgcttc ctgcaaacgc ccttcgattt cagagaggat tttttggatc
    14821 atctctttac ggttgccgcg cgcggctttc accacttctt taattacgcg gtaacgattg
    14881 ggatacagcg cctcaaaacc cagctcttcg agttcggttt taatgtggtg gatacctaaa
    14941 cggtgcgcca gcgggctata aatttcgaga gtttcacggg cgatgcggcg acgtttgtcc
    15001 gggcgaagtg agcccagcgt gcgcatgttg tgggtacggt cggcaagttt gatgagaatg
    15061 acgcggatat cctgcaccat cgccataatc atcttgcgaa agttttcggc ctgcgcctct
    15121 ttcttatcgc ggaacttgag cttatcaagt ttcgacaccc cctctaccag ctcggcgacg
    15181 cttttaccaa aaagctgttc catgtcctgg taggtggcgg gtgtatcttc aatcacgtca
    15241 tgcagcagcg ccgccattag cgtttcatag tcgagtttca tctcggccag aatgcaggca
    15301 accgctaccg ggtgcgtgat atagggttca ccgcttgaac gtgtttgccc ctcgtgagca
    15361 tcacgtgcaa cgagatacgc ctgccgcaga cgcttgattt ggtcttccgg caggtaggtt
    15421 tgaatcagtt gattcaggct ttcaaacaga tacaagggcg acccgctttg tgattaacga
    15481 cgaccttcag caatagcggt aacggcttgt aattcagcgg cttcctgctc ttgctgttcc
    15541 tggcgttcgc gaacgtcgag gatctggttg ttgatcagac cttcttcgat ttcgcgcagc
    15601 gcgattacag tggttttatc gttttcttcc ggtaccagcg gatcctttcc gcctacctgc
    15661 atctgacgag cgcgacgcgc ggcgaccagt accaggtcaa aacggttacc aattttctct
    15721 acagcgtcct gaacagttac gcgtgccata cttaaaaagc tccacaggtg aagaaatgac
    15781 tgggcatgat actgaaatca ggttcagtct gccaacaatt tgctgattaa agcgtcatga
    15841 cgctgctttt ggcggctcat gcgcagacgt tcggcgcgaa taatggtctt caaatcggtc
    15901 aacgcggtat cgaagtcatc attcacaatc aggtaatcat attcggcgta atggctcatt
    15961 tctgcaacag cttgcgccat acgctttgca atgacctctt cgctgtcctg accgcgaccg
    16021 cgtagacggc ggtccagttc aattttggac ggcggtaaaa taaagatact ccgcgcgtgc
    16081 ggcatcttct ggcgaatttg ctgcgcgccc tgccagtcga tatcgagaaa aacatcaaca
    16141 ccggtcgcca ggacttgctc aatggcctca cgcgaagtgc catagtaatt accaaaaact
    16201 tctgcgtgtt cgaggaacgc atctctgcta atcatttctt taaattcatc atgattaaca
    16261 aagaaataat gttcaccgtg gacttcacca ggacgcggtt ggcgtgtggt gtgtgaaaca
    16321 gaaacctggg tgtcatacaa cggttgggtt tttaataaag cctgaatcag gctggattta
    16381 cccgcgccac tgggggcaga aacaatataa agcgtgcctt gagccatgag tatctttcgt
    16441 atgtgattag cgaaataaag cctacatacg agcttattat acacggcgcg gccatgtgac
    16501 gtagcccttg ctcacacttt ttgcgcaaag ttgttgcgtt ttgcgtagct gcttccgcat
    16561 ttgcctgtca ttcactgcaa taaagacgat tttcaggaag cgtcctcgca gactccgttt
    16621 ttgccactcg gctttgcttt catcacgttg tatatctgcc tgactatcta cagcggaggt
    16681 gatgatgaaa gtatggatgg cgatattaat aagtatcttg tgctggcaat catctgtgtg
    16741 ggcggtctgt ccggcctggt cgccagccag agcacaggaa gaaatttccc gcctgcaaca
    16801 gcaaataaaa cagtgggacg atgactactg gaaggaagga aaaagtgagg tggaagacgg
    16861 tgtttacgat cagttaagcg cccgtcttac gcagtggcaa cgctgctttg ggagcgagcc
    16921 ccgcgacgtg atgatgccgc ctttaaatgg tgcggttatg catccggttg cccatacggg
    16981 tgtacgcaaa atggtggata aaatcgcatt aagtctgtgg atgcgcgaac gtagcgatct
    17041 ttgggtgcag ccaaaagttg atggcgttgc ggtaaccctg gtttatcggg acgggaaact
    17101 gaacaaagca atcagtcgcg gcaatggcct gaaaggcgag gactggacgc agaaagttcg
    17161 cttaatttcc gctgtgccgc aaaccgttag cgggccttta gccaacagta cgcttcaggg
    17221 ggaaatattt ctccagcgcg aggggcatat ccaacaacaa atggggggaa taaatgcccg
    17281 cgcaaaagtt gctggtctga tgatgcgcca ggacgatagc gacacgctga attctctggg
    17341 cgtttttgtc tgggcatggc cggatggacc gcagttaatg accgatcgtt taaaggagct
    17401 ggctaccgca ggttttactc taacgcagag gtatacccgt gcggttaaaa atgctgatga
    17461 ggttgcgcgc gtacgcaatg agtggaggaa agcgaaatta cccttcgtca ccgatggcgt
    17521 ggttgtgcga ggggctaagg aacccgaatc ccgtcattgg ctaccgggcc aggcggagtg
    17581 gctggtggcc tggaaatatc aacctgtagc tcaggttgcc gaagtgaagg cgattcagtt
    17641 tgcggtgggt aagagcggta aaatatcggt ggttgcgtca ctcgcacctg tcatgctgga
    17701 tgataaaaaa gtccagcggg tgaatattgg ttccgtcagg cgctggcagg agtgggatat
    17761 tgcacctggt gatcagatcc tcgtcagcct tgccggtcag ggtattcctc gcattgatga
    17821 tgtggtctgg cgcggtgcag aacgtacaaa accgacacca ccagaaaacc gctttaactc
    17881 gttgacctgc tactttgctt ctgatgtttg tcaggaacag ttcatttcac gcttagtctg
    17941 gctggggtca aaacaggttc ttgggctgga tggcattggt gaggccggct ggcgcgcgct
    18001 gcatcagact catcgctttg agcatatctt ttcctggctt ttattaacgc cagagcaatt
    18061 acagaacacg ccggggatcg cgaaaagtaa aagtgcgcag ctatggcatc agtttaatct
    18121 ggctcgtaag cagcctttta ctcgctgggt gatggcaatg ggaataccgc taacccgggc
    18181 ggcgcttaat gccagtgatg aacagtcctg gtcgcaactt ttatttagca cagaacagtt
    18241 ctggcagcag ctgccgggga ctggatctgg gcgcgccaga caggttattg aatggaagga
    18301 aaatgcgcaa atcaagaagc tgggcagttg gctggctgcc cagcagatca caggttttga
    18361 accttagtga ccttcgtggc tgtagtaaaa aaccggtaat cccagcttaa gacgtagcgc
    18421 cagtaaacgg gcaaaaaagc cgaataccag ggtggagata atcaccacat catgattaga
    18481 aacatagtgt tgcagcgcga tgtacagcac ggcggaggca aatgagacac cggcatacaa
    18541 ctctttctgg aataccagtg ggatgcgttt acagaacata tcgcgcaaaa cgccgccaaa
    18601 cacgccggtg gttaccgccg cgacaacggc aataattggg ccgtgcccca tatccagtgc
    18661 aacctgtgcg ccaatgatag aaaagaccac cagtccgagc gcatcgagca ccagaaacac
    18721 tttgcgcagg taaggcatta caggagcaac gatagtggtg agtactgcgg cggtagcgac
    18781 gataatcaca tattccgggt gtttgaccca gccaagcgga tagtggccca gcagaatatc
    18841 gcgcactgac cctccgccaa ttgcggtggc ggtcgcaata ataattacgc caaatgtatc
    18901 catgcgccgt cgcccggccg ccagcgcccc cgtcatggct tcggcagtaa tgccaaccaa
    18961 atacaaaatg tgtaacagca tggttctctc cctaaaaatc tgtctggcag ggtagcgatt
    19021 tgtgcgcatt gtcacgattg agatttttta aggtgagggg aaattgataa cttgtagtta
    19081 tgtgaaatcg ctattttctg tagcagagat gcattcttct gacttcttgt ctgagaaaat
    19141 gcataagaaa aaataggtta aaactgaggg gggaaaagaa aggtgcaata tttagccatt
    19201 gtgttcactg cacctggacc ttggcttacg catcgggtgt ctcaatcaac aaaaagagtt
    19261 tttagtttct gctatttctt ctctgtaatt tttacactgt tttccaactg cttgatgctg
    19321 atctgcgggg tcggcaactc ttcaggcatg gttccgccaa gttcctgaat ggtttgtctc
    19381 accttgcgac ccacgtcaaa gtgtgtggtg ttagcttgct gttttgaatt cacgccatcc
    19441 cgcttgagtt tttcttctgt ttgggtagct cgaaagagat tagccgccag ttctgttgaa
    19501 cccatatgat ccaggatctt ctgactcttt ttcagcccct tccgctgatg gatggctttc
    19561 tgatctaatc caccatacag cccccggtaa ccatgattct gaaatatggc gaaatcgata
    19621 gctgtcgcta ccccagcttg ctgcgctgcc tcaactaatt gtttgttatg ttctttcaat
    19681 tcatttctca gaaaaagacg cttttcgtct tcacgaagtt gcctgaaagc ttcatcgtca
    19741 gccagctcct gccgtcgggt ctggatagca aaataagttt gccctgccgc aataaccggt
    19801 ttcgcagggt cgccgttttg tactaccagg taacaggcat agcgggagag atgaacatct
    19861 tcaagttctc gttgagcccc tgaacctaat acaaccattt tggtggtttc aacgaaatgg
    19921 tcagatgcag cttgattgct ggcttcgcac gcttgagtcg cgcgggccag taccttttga
    19981 aaattgcgcc agtcccgata atccagtaac ggtgccaatt ctctcgctga ccaaaattct
    20041 tgcccttcag tgccataatg ccgtatttct tcaaaaggtt gatgatgttc gttcattgcc
    20101 cactccatgt gatatcgatt gcaaagaaag taaatcttta actgtattta tatacagttg
    20161 tgagtatctc acgttctcag tgtgctgggc aaaaagacat cgcaaaatct ggagcaggca
    20221 cgcaaaacag aattgaaagt tgcggggaag aaaaatgccg ctctgtttga gtgggcggca
    20281 tttgtgaagg ctgaaaagta agaattggcg ttacgagtta ttcaatgttc tgaatctgct
    20341 cacgcatttg ctcaatcaac actttcagct cgatggcaga gtttgtcact tcggcattga
    20401 tagatttcga cgcaagagtg ttcgactcgc ggttgaactc ctgcatcata aagtccagac
    20461 gacggccaac cgcttctttt ttcttcagaa tgttgtaggt ctctttaaca tgcgcttcga
    20521 ggcggtccag ttcttcggca acgtcaattc gttgtgccag cagaaccaac tcctgctcca
    20581 gacggttgtt ttccaactgc acctgagcat cttccagctt cgcgaccaag cgttcacgct
    20641 gccattgcag gatttccggc atatgggagc ggactttgac cacttcggcg gtgacgcctt
    20701 ccagacgctg ctcaatcaac gctttcagcg cctgaccttc ggtttcgcga gcgatgataa
    20761 agtcatccag ggtgccatcc aacgccgcga gaatttcagc ggcaatcgcg tcgagatcct
    20821 gctcctgggc tgccatcacg cccggccagc gtagaatatc aaccgggttg atttcccctt
    20881 cgtcactctg catttttacc cagttcgcgg cagttaccag ctgtttagcc agtttttcat
    20941 tgaggatcag ctcaccttgt gcgctaacat ctggctcata gcgcagggta cattccactt
    21001 taccgcgcgt caggcgagaa cgaatacgct cacgaacgac aggttcaagg ctacggaact
    21061 gctccggcag acgaaagtaa gtttccagat aacgctggtt taccgagcgc atttcccagg
    21121 ttgcgctccc ccattcaccc ttgatttcac gccgggcgta ggcggtcata ctgcggatca
    21181 tagacgttcc tgtttataaa aggagaggtg gaaggattat agccatcgat gccttgtcag
    21241 gataggaata accgccggaa gtccgtataa tgcgcagcca catttgtttc aagccggaga
    21301 tttcaatatg cgtccagcag gccgtagcaa taatcaggtg cgtcccgtta ccctgactcg
    21361 taactataca aaacatgcag aaggctcggt gctggtcgaa tttggcgata ccaaagtgtt
    21421 gtgtaccgcc tctattgaag aaggcgtgcc gcgcttcctg aaaggtcagg gccagggctg
    21481 gatcaccgca gagtacggca tgctgccacg ttctacccac acccgtaacg ctcgtgaagc
    21541 ggcgaaaggt aagcagggtg gacgcacaat ggaaatccag cgtctgatcg cccgtgctct
    21601 tcgcgcggca gtagatttga aagcgctggg tgagttcacc attacgctgg actgcgacgt
    21661 gcttcaggct gatggtggca cgcgtaccgc gtcgattacg ggtgcctgcg tggcgctggc
    21721 agatgcgcta cagaagctgg tggaaaacgg caagctgaaa accaacccga tgaaagggat
    21781 ggtagccgca gtttctgtcg gaattgtgaa cggcgaagcg gtttgcgatc tggaatacgt
    21841 tgaagactca gccgcagaga ccgacatgaa cgtagtgatg accgaagacg ggcgcatcat
    21901 tgaagtgcag gggacggcag aaggcgagcc gttcacccat gaagagttac tcaccttgtt
    21961 ggcgctggcc cgagggggaa tcgaatccat tgtagcgacg cagaaggcgg cgctggcaaa
    22021 ctgattttta aggcgactga tgagtcgcct tttttttgtc tgtagaaaag taagatgagg
    22081 agcgaaggca tgaaaccata tcagcgccag tttattgaat ttgcgcttag caagcaggtg
    22141 ttaaagtttg gcgagtttac gctgaaatcc gggcgtaaaa gcccctattt cttcaacgcc
    22201 gggctgttta ataccgggcg cgatctggca ctgttaggcc gtttttacgc tgaagcattg
    22261 gtggattccg gcattgagtt cgatctgctg tttggccctg cttacaaagg gatcccgatt
    22321 gctaccacaa ccgccgtggc gctggcggag catcatgacc tggacctgcc gtactgcttt
    22381 aaccgcaaag aggcaaaaga ccacggtgaa ggcggcaatc tggtcggtag cgcgttacaa
    22441 ggacgcgtaa tgctggtgga tgatgtgatc accgccggaa cggcgattcg cgagtcgatg
    22501 gaaattattc aggccaatgg cgcgacgctt gctggcgtat tgatttcgct cgatcgtcag
    22561 gaacgcgggc gcggcgagat ttcggcgatt caggaagttg agcgtgatta caactgcaaa
    22621 gtgatctcta tcattaccct gaaagacttg attgcttacc tggaagagaa gccagaaatg
    22681 gcggaacatc tggcggcggt taaggcctat cgcgaagagt ttggcgttta aagaaactcg
    22741 ccggatgaaa agtcatccgg cgtcatatta ctgcaactgc gcggcaatta gcggccagcg
    22801 ggcgtcaaaa tcatccgtcg ggcgatattt aaattcgctg cggacaaaac gtgacagcat
    22861 accttcacag aaggccagca actggcttgc cagcagcgtt tcatcagtgg tgtaaccttc
    22921 accctcacgc attctctttt cacgcaatac ctgacgcagc tgcgcttcaa tacgttcgaa
    22981 aagttggttg atgcgccctt gcaggcggtc ttgttcaaac atcagcgcat gcccggtaag
    23041 gatgcgggtc aggccaggat tacgctcacc aaaaccgagg agcaacaaca cgatcagacg
    23101 caggcgcgct gtggtgtctt tctcatcttt cagaatcaaa ttgatgcgag taatcaggct
    23161 atcttcgata aactcaatca ggctatcgaa catgcgggtc ttactgggga agtggcgata
    23221 cagtgccgct tcggaaacgc cgacagaggc ggccagtttt gccgtcgtga tacgttggct
    23281 tccatcgctg gattccagca tcagcgccag agactgaagt atttcctcgc gacggttcct
    23341 tttcgcagtt tgtttttctg ccatgttaca aaatacccct gaaaataagc acttgccagg
    23401 cggcacccac gctatgaccg caaacgaaat gtttgcggct atgttatgac gttatttgga
    23461 tgcgtatgtg ttactgacga ccagagtgac caaagccgcc ttcaccgcgg tcggtggcgt
    23521 cgaaatcttc caccagatta aattcagcct gtactaccgg aacaaaaatc atctgtgcga
    23581 tacgttcacc cggttgaatg gtgaagctgt cctgaccacg gttccacacg gaaatcatca
    23641 actggccctg atagtcggaa tcgatcaatc ctaccaggtt accaagcacg ataccgtgct
    23701 tatgtcccaa tccggagcgc ggcagcatca ttgccgccag tgaaggatcg gcaatatgaa
    23761 tcgccagccc ggtcggaacc agcgcagtgt cacccggagc cagttctacg gcgtcatcga
    23821 gacaggcacg caggtcaagt ccggcagagc cagaggtggc ataagtcggg agcggaaatt
    23881 ccttcccaac gcgcgggtcc agaatcttaa cgtcgatttt tttcatcata acgggtcacg
    23941 atctcgtcga gtaataattg gccaaggagc tctttgcgct caagcggtaa gactttatct
    24001 ccgtcctgcc agaaaaggtg taatgcgttg ttgtcgctgt taaatccttg agttggctgg
    24061 gaaacatcgt tcgcgcagat cagatcaagg tttttacgga tacgtttttg ccgggcgtat
    24121 tcttccacat tatttgtttc ggcggcaaat ccaacgacgt agggtcgatg gtcttttagt
    24181 gcggcaacgc ctgcgacgat atcggggttt ttaaccattt ttattgttaa ttcatcaccc
    24241 tgcgtggcct gctttttgat tttttctggg gccacggtag ctgcgcgata atccgccacg
    24301 gcggcgcaac cgataaaaat attttgctgc tgtacagaag cattcacggc ggcttccatt
    24361 tccagcgcgg tcatcacatc aacacgttta acaaacggtg gcgtcggtag tgaaactgga
    24421 cctgatacca gcgtgacgtt cgcgccacga cgggcagcgg cggcggcgat agcaaaaccc
    24481 atcttgccgg agctgtgatt agagatataa cgcaccggat cgagcggttc acgcgtcggg
    24541 ccggcggtaa tcataatgtt cagatgtttt aggtcgttga cgggcgaaaa atgcgctact
    24601 gccatatcca caatggttaa cggatcgagc attcgcccag gaccgatatc accacaagcc
    24661 tgactgccac tgtctggccc ccagatgagc aaaccacggg aagcaagcac ctctaaatta
    24721 tgctgcgtgg cagcggcacg gtacatctgc tggttcatgg cggggagcac ggctacaggc
    24781 gcaggtgtag ccagacaaat cgtcgatacc aggtcattcg ccattccggc agcaacacgg
    24841 gcaatcaaat ctgccgtggc aggggtgaga atcactaaat cagcccattt acccagctca
    24901 atatggccca tagcggcttc ggctgccggg tccagcaaac tgtcggaaac gggataacca
    24961 gaaaccgcct gcaagctaag tggggtgata aaggcttttg ccgcttcggt catggctacg
    25021 cggacgtcgg ccccgcgatc gcgcaaacga cgcaccagtt caggggtttt ataggcagca
    25081 ataccgccgc taacgccgag aacgattttt ttaccggcca ggctcatgat gatttttcct
    25141 gtagggtgac accagaagtt ggcgatttta tcacaatact tttgttgtcg tgccttcacc
    25201 tgagaatcac tttgcgaggc gctttccagg attgaaaact ggccgtcgat ttaacggaac
    25261 ggctatgaca ggatgcgagc accacaaagg aggtgaaggt gaaaaacaat gcacagctgt
    25321 tgatgccgcg cgaaaaaatg ctgaagtttg gtattagcac cttaacggat gtcgagctgc
    25381 tggcgctatt tctgcgtacc ggaacgcgcg gtaaagatgt attaaccctg gcaaaagaga
    25441 tgctggagaa tttcggctct ctttatggct tgttaacctc tgaatatgaa caatttagtg
    25501 gtgttcatgg aattggcgta gcgaaatttg cccagttaaa ggggattgct gaactggcgc
    25561 ggcgttacta caacgtgcgg atgcgtgaag aaagcccttt actcagcccg gagatgacgc
    25621 gggaattttt acaaagccag ctcacgggtg aggagcggga gatctttatg gtgatctttc
    25681 tcgactcaca acaccgggtt ataacgcata gccgtctttt ttccggcacg ctaaaccatg
    25741 ttgaagtcca tcctcgggaa attatccgcg aagcgataaa aataaacgcc tcggcgctga
    25801 tccttgcaca taatcaccct tcgggttgtg ctgaacccag taaagcggat aaactcatta
    25861 ctgaacggat aataaagagt tgtcagttca tggatttacg cgtgctcgac catatcgtga
    25921 ttgggcgtgg agagtatgtt tcttttgccg aacgcggctg gatttaaccc gctatgcgcg
    25981 atccttcggg atctttgtct gttcgggact tgagcacatc gctgagtcag cgtatactac
    26041 gccacctttg agaatctcgg gtttggcatt tgggcctggc aatcgagagt tcacatagaa
    26101 ctgcgatgac cgggctgtaa agcctgacga ggcgccaata ccccatacga agctcgagct
    26161 aatttgattt ttggagaata gacatgtccc gagtctgcca agttactggc aagcgtccgg
    26221 tgaccggtaa caaccgttcc cacgcactga acgcgactaa acgccgtttc ctgccgaacc
    26281 tgcactctca ccgtttctgg gttgagagcg agaagcgttt tgtcaccctg cgcgtatctg
    26341 ctaaaggtat gcgtgtaatc gataaaaaag gcatcgatac agttctggct gaactgcgtg
    26401 cccgtggcga aaagtactaa gtacttagag gaaataaatc atggctaaag gtattcgtga
    26461 gaaaatcaag ctggtttctt ctgctggtac tggtcacttc tataccacta cgaagaacaa
    26521 acgtactaag ccggaaaaac tggaactgaa aaaattcgat ccagttgttc gccagcacgt
    26581 gatctacaaa gaagcgaaaa tcaaataatt ctcgctttga tgtaacaaaa aacctcgctc
    26641 cggcggggtt ttttgttatc tgcttgcccc catattgact gcatctgttc attcctggag
    26701 atgctatgcc tgaattaccc gaagttgaaa ccagccgccg cggcatagaa ccgcatctcg
    26761 ttggtgcaac cattcttcat gcggtggtgc gcaacggacg cttgcgctgg ccggtttcag
    26821 aagagatcta ccgattaagc gatcaaccag tgcttagcgt gcagcggcgg gctaaatatc
    26881 tgctgctgga gctgcctgag ggctggatta tcattcattt ggggatgtct ggcagcctgc
    26941 gcatccttcc agaagaactt ccccctgaaa agcatgacca tgtggatttg gtgatgagca
    27001 acggtaaagt gctgcgctac accgatccgc gccgctttgg tgcctggctg tggaccaaag
    27061 agctggaagg gcataatgtg ctggcccatc ttggaccgga gccgcttagc gacgatttca
    27121 acggtgagta cctgcatcag aagtgcgcga agaaaaaaac ggcgattaaa ccgtggctga
    27181 tggataacaa gctggtggta ggggtaggga atatctatgc cagcgaatct ctgtttgcgg
    27241 cggggatcca tccggatcgg ctggcgtcat cactgtcgct ggcggagtgc gaattgctgg
    27301 ctcgggtgat taaagcggtg ttgctgcgtt cgattgagca gggcggtaca acgctaaaag
    27361 attttctgca aagtgatggt aaaccgggct atttcgcgca ggaattgcag gtttacgggc
    27421 gaaaaggcga gccctgtcgg gtgtgcggta cgccgattgt ggcgactaaa catgcgcagc
    27481 gggcaacgtt ttattgtcgg cagtgccaga agtaattcat gcgcgccgga tggcatacca
    27541 tccggcataa acgctacgct aacttcgcca tcagcgcctg atggacattc tccggcagga
    27601 aatgggtgac atcgccctga tggcgcgcca cctctttcac caacgatgaa gagataaacg
    27661 accactcttt cgacggcatc agaaacacac tttccagttc cggcattaag tggcgattca
    27721 tatgcgccag ctgcatttca tattcaaaat ccgccaccgc acgcaggcca cgaatcagca
    27781 ccgtagcgtg ttgattacgg gcgaagttcg ccatcaaatc actaaacccg accacttcca
    27841 cgttccccag atgcgcggtt gcctgctgtg ccagctcaac acgctcttcc agggtaaaca
    27901 tcggtttttt actggggctg gcggcaatcg ccagaataac gtgatcgaac atctgcgtgg
    27961 cgcgcgtcac gatatcgata tgaccattgg taatgggatc gaaagtaccc ggataaatcg
    28021 cccgtttttg cataacaacc tcaatgcgtt ttcggtggca ggtaaggttc cagcagttga
    28081 agcagacgct gtagcgcgcc ctggttttga tacagtactt caacggcatg acggccatag
    28141 aaactacggt aatcggcgtc ggtgagtaaa gaggaaacct cttttgcaag cgacgtggca
    28201 tcggtaacgg taatcagccc gcttgcctgc tccagccgcg cgcaaatgtc tttaaagtta
    28261 aaagtatgcg gccccatcaa taccggaata gcgtgtgcgg cagcttccag cggattatgc
    28321 ccaccacgtt caaccagtga accgccaaca aaggcgagat cggcaatgcc atacagtaac
    28381 atcaactcgc ccatcgtatc gccaaccaca acctgcgtgc tggtggaggg gacttcccct
    28441 gaggagcgtg tgatatagct tagtccagcc tggcggacaa ggttaatcgc atccgggaag
    28501 cgttccggat gacggggtac caggatgagc aataaattcg ggaattgctg taacaatgcc
    28561 tgatgtgcgg cgatcaccac actctcttcg ccttcgtgag tgctggtggc aatccatacc
    28621 gggcggtgtg gtgcccattg gcggcgcagc gtcacggctt tagcagccaa ctgcggcgtt
    28681 acagaaatat cgaatttcag gctaccggta acggttacct ggttattttt tgcgcccagc
    28741 gccacaaaac gtgcaccatc ttcttcattt tgggcggcaa tcagcgtaat acgacgcagc
    28801 aagcgacgga cgaatttacc cagtttggca taacctgcgg ccgagcgggc agagagtcgc
    28861 gcgttagcga tcaccagcgg aattttacgt ttatgtagcg ccgcaatcag gttaggccat
    28921 agttcggttt ccataatcaa caccagttta gggtcgactt tattcaggaa acggttgagt
    28981 gcatcgggca gatcatacgg cagataaacg tgctgaacat cctttccgaa agccgattgt
    29041 acgcgctccg aaccggttgg cgtcatggtt gttacggtaa tcggtaaatc aggataacga
    29101 tgacgcagcg cgcgcaccaa cgggattgcc gccagagttt caccgacgga gacggagtgc
    29161 agcataatgc cgcctggttt tagcggatgg cggtaaaaac cgtaacgttc accccagcgt
    29221 tttcgatagg ccggagcctt acgtccgcgc acccagagcc gtatccagat cagcggctga
    29281 ataaggtaga gaagggcggt gtaaagcaat tcgagcatag taaatagctg acttatggat
    29341 gtgctgggga ttctatgtat ttagctgtgg ctttaccatt acttttcccg tttttgactt
    29401 aaatagcaat ttacccgcca attcttgctg aacatctata ctttttttgt atttttatgc
    29461 gattcattga tttctgctgt acgtgcagga aggaatcgca gagcaacagt gaaagaatat
    29521 ggttgctaat gattatattt gattgatttc tttattgaaa atcaacagtg tgcttaaaaa
    29581 tcattcgggt cggattgccg cgaaagaagt gatacactag cgcgtcaata tcagtgcggt
    29641 atcagtattc aggtagctgt tgagcctggg gcggtagcgt gctttttctg cttcacctta
    29701 atcggataat ctcaacaaaa agagttactt gtggataagc catttcgaag aattttgctc
    29761 attaagatgc gttttcatgg ggatatgtta ttaactactc ccgtcattag ttcgctgaaa
    29821 aaaaattacc ctgacgcaaa aatcgatgtg ctgctttatc aggacaccat cccgatcctg
    29881 tctgaaaatc cagagattaa cgcgctctac ggcataaaaa ataaaaaagc aaaagcctca
    29941 gaaaaaattg ccaacttttt tcatctcatc aaggtattac gtgccaataa gtatgacctt
    30001 atcgtcaatc ttaccgatca atggatggtt gctatactgg ttcgcttatt aaatgcccgt
    30061 gtgaaaattt cccaggatta tcatcatcgg cagtctgctt tttggcgtaa aagtttcacc
    30121 catttggtgc cgttgcaggg tggaaatgtg gtggaaagta acttatccgt gctgacacca
    30181 ttgggacttg attcgttggt gaagcagaca accatgagtt acccgcctgc aagctggaaa
    30241 cgtatgcgtc gcgaacttga tcacgctggt gttggacaaa attatgtggt tatccaacct
    30301 acggcgcggc aaatcttcaa atgctgggac aacgccaagt tttccgctgt gattgatgcc
    30361 ttacatgctc gtggttatga agtcgttctg acgtccggcc cagataaaga cgatctggcc
    30421 tgcgtcaatg aaattgcgca gggatgccag acgccaccag taacggcgct ggctggaaag
    30481 gtgaccttcc cggaacttgg tgcgttaatc gatcatgcgc agctgtttat tggcgttgat
    30541 tccgcaccgg cgcatattgc cgctgcagtt aatacgccgc tgatatcgct gtttggtgcg
    30601 acagaccata ttttctggcg tccctggtca aataacatga ttcaattctg ggcgggagat
    30661 taccgggaaa tgccaacgcg cgatcagcgt gaccgaaatg agatgtatct ttcggttatt
    30721 ccggcggcag atgtcattgc tgctgtcgat aaattactgc cctcctccac gacaggtacg
    30781 tcgttatgat cgttgctttt tgtttatata aatattttcc ctttggcggt ttgcagcgcg
    30841 attttatgcg tattgctcag acagtcgccg cccgaggtca tcatgttcgg gtttataccc
    30901 agtcgtggga aggcgaatgc cctgatgtat ttgaactgat caaagtgccg gttaaatcgc
    30961 ataccaatca cgggcgcaat gcggagtatt ttgcctgggt gcaaaaacat ttacgcgaac
    31021 atcccgtcga taaagtcgtt ggattcaaca aaatgccggg gctggacgtt tattatgccg
    31081 ctgatgtttg ttatgccgag aaagtagcgc aggaaaaagg ctttttctat cgcctgacgt
    31141 cacgttatcg ccattatgcc gcctttgagc gagcaacctt cgaacagggc aagccgacac
    31201 agctgctaat gctgacagat aagcaaatcg ccgatttcca gaaacattat cagactgaag
    31261 cggagcgttt tcatattctg ccaccgggga tttatcctga tcgtaaatat agccagcagc
    31321 cagccaatag ccgtgaaatc ttccgtaaga agaatggaat aaccgaacaa caatatttat
    31381 tgttgcaggt cggttcagac ttcacgcgta aaggtgtcga tcgttccatt gaagcacttg
    31441 cttcgttacc ggattcgctg cgccacaaca cattgctata tgttgttggg caggataaac
    31501 cgcgaaaatt tgaggcgctg gcagaaaaac gcggcgtgcg cagtaatgtt cacttcttct
    31561 cggggcgcaa cgatgtctcg gaattaatgg cggcggcgga tttattactg catcctgcct
    31621 accaggaagc ggcgggaatt gtgctgctgg aagcgattac tgcaggatta ccagtactaa
    31681 caacagccgt ttgtggctat gcgcattata ttgtcgaggc caattgcggc gaggctattg
    31741 ttgagccatt ccgccaggaa acattgaatg agattttacg caaagcgtta acgcaatctt
    31801 cattgcgcca ggcctgggcg gaaaatgcgc gacattatgc tgatacacaa gatttataca
    31861 gtctgccaga gaaagcggcg gatatcataa cgggtggtct ggatggttga acttaaagag
    31921 ccgtttgcca cgttatggcg cggtaaagat ccttttgagg aagttaaaac cttgcagggt
    31981 gaggtatttc gtgaactgga aactcgccgt actctgcgct ttgaaatggc gggcaaaagc
    32041 tattttctca aatggcatcg cggcacgacc ctgaaagaga taatcaaaaa tttactctca
    32101 ttgcggatgc cagtattagg cgcagaccgc gaatggaatg cgattcatcg actgcgggat
    32161 gtcggcgttg atactatgta tggggtggca tttggcgaaa aaggcatgaa tccgctgacc
    32221 agaacttcat ttattattac cgaagatctg acaccaacca taagtctgga agattactgt
    32281 gctgactggg cgactaaccc tccagatgtt cgcgtaaagc gtatgcttat taagcgtgtc
    32341 gcgacgatgg tgcgcgatat gcatgctgcg ggcattaacc accgtgactg ttatatctgt
    32401 catttcctgc tgcacttgcc tttttccggt aaggaagagg agttaaaaat ttcggtaatt
    32461 gacctgcacc gggcgcagct tcgcacgcgc gttccacgtc gttggcgcga taaagatctt
    32521 attgggcttt atttttcttc gatgaatatc ggcctgactc agcaggatat ctggcggttt
    32581 atgaaagtgt attttgccgc cccgcttaaa gacattctca agcaggaaca aggactgctg
    32641 tcgcaagcag aagcaaaagc cacaaaaatc agggaaagaa cgattcgaaa atcgttgtaa
    32701 tttccggaga gaaatatgag tgcccactat tttaatccac aagagatgat caataagaca
    32761 atcatcttcg atgaaaggcc agcggcgtca gtggcatcat cattccatgt tgcttatggc
    32821 attgataaaa actttctttt tggttgtggt gtttcaatca cgtcagtttt gttacataac
    32881 aacgacgtga gttttgtttt ccacgttttt attgatgata tccctgaagc cgatatccag
    32941 cgtttagccc aattggcgaa aagctatcgt acctgtatcc agatccatct agtaaattgt
    33001 gaacggctta aggcattacc gacgaccaaa aattggtcta ttgccatgta tttccgtttt
    33061 gtaattgcag attactttat tgatcaacaa gataagatcc tttacctgga tgctgatatc
    33121 gcctgtcagg gaaacttaaa gacgctgata acaatggatc ttgccaataa cgttgctgct
    33181 gttgttactg aacgcgatgc taactggtgg tcgttacggg ctcaaagtct gcagtgtaat
    33241 gaacttgaaa agggttactt taattcaggt gtcctgttaa ttaatacact agcgtgggcg
    33301 caggagtccg tttctgctaa agcgatgtcg atgcttgctg ataaagccgt cgtttcccgt
    33361 ttaacctata tggatcaaga tatccttaat cttat
//
LOCUS       DAZUCE010000023        33329 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZUCE010000023 DAZUCE010000000
VERSION     DAZUCE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.023"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            59..1114
                     /gene="phoE"
                     /locus_tag="ACHY03_001014"
     CDS             59..1114
                     /gene="phoE"
                     /locus_tag="ACHY03_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFS3904277.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1153..1554)
                     /gene="crl"
                     /locus_tag="ACHY03_001015"
     CDS             complement(1153..1554)
                     /gene="crl"
                     /locus_tag="ACHY03_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFS3904278.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1612..2856)
                     /gene="frsA"
                     /locus_tag="ACHY03_001016"
     CDS             complement(1612..2856)
                     /gene="frsA"
                     /locus_tag="ACHY03_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFS3904279.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2948..3406)
                     /gene="gpt"
                     /locus_tag="ACHY03_001017"
     CDS             complement(2948..3406)
                     /gene="gpt"
                     /locus_tag="ACHY03_001017"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS3904280.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3667..5124
                     /gene="pepD"
                     /locus_tag="ACHY03_001018"
     CDS             3667..5124
                     /gene="pepD"
                     /locus_tag="ACHY03_001018"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFS3904281.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPTK"
     gene            complement(5181..5702)
                     /gene="prfH"
                     /locus_tag="ACHY03_001019"
                     /pseudo
     CDS             complement(5181..5702)
                     /gene="prfH"
                     /locus_tag="ACHY03_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5701..5904)
                     /locus_tag="ACHY03_001020"
                     /pseudo
     CDS             complement(5701..5904)
                     /locus_tag="ACHY03_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(6150..6602)
                     /gene="yafP"
                     /locus_tag="ACHY03_001021"
     CDS             complement(6150..6602)
                     /gene="yafP"
                     /locus_tag="ACHY03_001021"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3904282.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPWIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            complement(6599..7654)
                     /gene="dinB"
                     /locus_tag="ACHY03_001022"
     CDS             complement(6599..7654)
                     /gene="dinB"
                     /locus_tag="ACHY03_001022"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFS3904283.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(7725..8648)
                     /gene="lafU"
                     /locus_tag="ACHY03_001023"
     CDS             complement(7725..8648)
                     /gene="lafU"
                     /locus_tag="ACHY03_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFS3904284.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(8651..9514)
                     /gene="motA"
                     /locus_tag="ACHY03_001024"
     CDS             complement(8651..9514)
                     /gene="motA"
                     /locus_tag="ACHY03_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFS3904285.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(9527..10243)
                     /locus_tag="ACHY03_001025"
     CDS             complement(9527..10243)
                     /locus_tag="ACHY03_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFS3904286.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWPELVVEGISYEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQHALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(10263..10730)
                     /locus_tag="ACHY03_001026"
     CDS             complement(10263..10730)
                     /locus_tag="ACHY03_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFS3904287.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(10738..11799)
                     /locus_tag="ACHY03_001027"
     CDS             complement(10738..11799)
                     /locus_tag="ACHY03_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFS3904288.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATCPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPDQQAKLATYASEDLHAIAPTQ
                     QRAPAKPERAKSEPTRVIVRPPVVRKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNVKLMV
                     HIGANQSEVCRALQQFSDDLRQHLMAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(11796..12107)
                     /locus_tag="ACHY03_001028"
     CDS             complement(11796..12107)
                     /locus_tag="ACHY03_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFS3904289.1"
                     /translation="MEKQRRQLFALVEAMNEALDKQRWRRLPALHQQLMRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNTLIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(12112..12504)
                     /gene="fliS"
                     /locus_tag="ACHY03_001029"
     CDS             complement(12112..12504)
                     /gene="fliS"
                     /locus_tag="ACHY03_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFS3904290.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(12527..13843)
                     /gene="fliD"
                     /locus_tag="ACHY03_001030"
     CDS             complement(12527..13843)
                     /gene="fliD"
                     /locus_tag="ACHY03_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFS3904291.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTVDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDTSNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPASTNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYERYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(14134..15048)
                     /gene="lafA"
                     /locus_tag="ACHY03_001031"
     CDS             complement(14134..15048)
                     /gene="lafA"
                     /locus_tag="ACHY03_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFS3904292.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            15534..16376
                     /locus_tag="ACHY03_001032"
     CDS             15534..16376
                     /locus_tag="ACHY03_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFS3904293.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSFLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPESSPAEALIITDQIKETVPEEISSTTPPVPVRKKHKGLMGLALTAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLTSAGENNSAPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVDSWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(16679..17656)
                     /locus_tag="ACHY03_001033"
     CDS             complement(16679..17656)
                     /locus_tag="ACHY03_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFS3904294.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPVTASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLIHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAGQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPDIERTLSVRTDGDK
                     SAFMPLKTFAEPSEAERLKQSLQQGGAGISQILENINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(17673..18602)
                     /gene="flgL"
                     /locus_tag="ACHY03_001034"
     CDS             complement(17673..18602)
                     /gene="flgL"
                     /locus_tag="ACHY03_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFS3904295.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKEILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDRPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(18617..19993)
                     /gene="flgK"
                     /locus_tag="ACHY03_001035"
     CDS             complement(18617..19993)
                     /gene="flgK"
                     /locus_tag="ACHY03_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFS3904296.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALRQQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTPTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIDNLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSEAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(20474..20773)
                     /locus_tag="ACHY03_001036"
     CDS             complement(20474..20773)
                     /locus_tag="ACHY03_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFS3904297.1"
                     /translation="MKVNASGGIDGSDALMGPKVRANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(20773..21873)
                     /locus_tag="ACHY03_001037"
     CDS             complement(20773..21873)
                     /locus_tag="ACHY03_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFS3904298.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(21888..22553)
                     /gene="flgH"
                     /locus_tag="ACHY03_001038"
     CDS             complement(21888..22553)
                     /gene="flgH"
                     /locus_tag="ACHY03_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFS3904299.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKNNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(22883..23668)
                     /gene="flgG"
                     /locus_tag="ACHY03_001039"
     CDS             complement(22883..23668)
                     /gene="flgG"
                     /locus_tag="ACHY03_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFS3904300.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDPTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(23847..24584)
                     /locus_tag="ACHY03_001040"
     CDS             complement(23847..24584)
                     /locus_tag="ACHY03_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFS3904301.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTGDGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(24584..25786)
                     /gene="flgE"
                     /locus_tag="ACHY03_001041"
     CDS             complement(24584..25786)
                     /gene="flgE"
                     /locus_tag="ACHY03_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFS3904302.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKDGYIVNASGDYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSNLTFTANFNASD
                     DAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPIDLTVDYTTCTQYGSDFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(25930..26643)
                     /locus_tag="ACHY03_001042"
     CDS             complement(25930..26643)
                     /locus_tag="ACHY03_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFS3904303.1"
                     /translation="MNTLALNAQSPQPTATAENNTVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(26643..27074)
                     /gene="flgC"
                     /locus_tag="ACHY03_001043"
     CDS             complement(26643..27074)
                     /gene="flgC"
                     /locus_tag="ACHY03_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFS3904304.1"
                     /translation="MSFTDIYQISGSAMTAQSIRLNTVASNLANAEAPAGSEAQAYKA
                     RSPVFAAVYHNSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(27077..27412)
                     /locus_tag="ACHY03_001044"
     CDS             complement(27077..27412)
                     /locus_tag="ACHY03_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFS3904305.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            27494..28231
                     /gene="flgA"
                     /locus_tag="ACHY03_001045"
     CDS             27494..28231
                     /gene="flgA"
                     /locus_tag="ACHY03_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFS3904306.1"
                     /translation="MSFQFCRRKFYFLLALAIPGYAAVHPVQHSAREQVNAQVLNAAS
                     QQIESLAQQRQWHDYRYIFKVYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMAKSVVARDTPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPLTKEELVSPVLVERDQPVMIVYQNAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            28312..28590
                     /gene="flgM"
                     /locus_tag="ACHY03_001046"
     CDS             28312..28590
                     /gene="flgM"
                     /locus_tag="ACHY03_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFS3904307.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKSTATTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQIQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            28603..29031
                     /locus_tag="ACHY03_001047"
     CDS             28603..29031
                     /locus_tag="ACHY03_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HFS3904308.1"
                     /translation="MSIRLQQVKALLQGIRDDNKNYDGLRALLEQQRLCMIRRASDEL
                     LAVNETIMQHYERLNSTSLQRRSLLQLLGVSVNRAGLEQVFGWLPAAQNNAARHWWQR
                     LEQKAGSCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            29159..30088
                     /locus_tag="ACHY03_001048"
     CDS             29159..30088
                     /locus_tag="ACHY03_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFS3904309.1"
                     /translation="MSFIECYEPEYVRNFMTQHPGSPLYVRKVWKNEIRLSLMLTEPE
                     SCEAMLNDARAFDLQLTYRSVEGNAAELSARDAILNQVILSTLTLPNLPPELSLYAVG
                     ILLSRASKMPGRDGDILARLTTLPQALGDHAKKGTLQAQFAQLPSVPQLARQLMTLLG
                     SCAFNWSILPESPRKTSLPLQMPLLTLHDANSEALLQQQLQGQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDVIGQTDGADYCPLVCDFYLIRTLISLWTLDGSPLRQDDIFALFAMF
                     ERWRVSENAAAIRQQIQSLCAAEPLLSAFSLLT"
     gene            30202..32646
                     /locus_tag="ACHY03_001049"
     CDS             30202..32646
                     /locus_tag="ACHY03_001049"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS3904310.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRATFLPYLLYMYRYQDY
                     PADRRELVILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELAVG
                     EYILCMDDDDYYPADKISWTIAMMQKHRALISGSDQIPIWYSHISRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFILGASTPVDAALTDIVSDPLLRNAYLSLHNATHHQAINHQAIDQVIIVNLDKRPDR
                     LQQIREELALLHIPPEKIIRLAASEDENGQRGRRQSHLQALRLAQQRGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSICYQRPGFSDIEKKTTDN
                     IAHYFNKLPVATKPSTLPIADTIGFFMETSFHYTLYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQAHGQRVKCLVSPYHTPALNGLSPVNIRAM
                     YGLAKETWNHADWNRFYQRILCYSHYSQQALEHFGSAKVVGNPRFDAWHNGTFDRALP
                     GNIELDDRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     TLAHRHLKQRTNSAPHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMTELLD
                     GEKSYSTPESADQRIREILPVAHNVAELRYLLSEAFDWGAVQTKLAEIRHHYCDAFMD
                     GKAGERAAMVIVEALG"
     gene            33044..33193
                     /locus_tag="ACHY03_001050"
                     /pseudo
     CDS             33044..33193
                     /locus_tag="ACHY03_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         7437 a         9150 c         8693 g         8049 t
ORIGIN      
        1 gttttcaacg gggctctccc gctgaatatt cgcgcgttaa ttaaaatcag gaatgaaaat
       61 gaaaaagagc actctggcat tagtggtgat gggcattgtg gcatctgcat ccgtacaggc
      121 cgcagaaata tataacaaag acggtaataa actggatgtc tatggcaaag ttaaagccat
      181 gcattatatg agtgataacg acagtaaaga tggcgaccag agttatatcc gttttggttt
      241 taaaggcgaa acacaaatta acgatcaact gactggttat ggtcgttggg aagcagagtt
      301 tgccggtaat aaagcagaga gtgatactgc acagcaaaaa acgcgtctcg cttttgccgg
      361 gttgaaatat aaagatttgg gttctttcga ttatggtcgt aacctgggcg cgttgtatga
      421 cgtggaagcc tggaccgata tgttcccgga atttggtggc gactcctcgg cgcagaccga
      481 caactttatg accaaacgcg ccagcggtct ggcgacgtat cggaacaccg acttcttcgg
      541 cgttatcgat ggcctgaact taaccctgca atatcaaggg aaaaacgaaa accgcgacgt
      601 taaaaagcaa aacggcgatg gcttcggcac gtcattgaca tatgactttg gcggcagcga
      661 tttcgccatt agtggggcct ataccaactc agatcgcacc aacgagcaga acctgcaaag
      721 ccgtggcaca ggcaagcgtg cagaagcatg ggctacaggt ctgaaatacg atgccaataa
      781 tatttatctg gcaacttttt attctgaaac acgcaaaatg acgccaataa ctggcggctt
      841 tgccaataag acacagaact ttgaagcggt cgctcaatac cagtttgact ttggtctgcg
      901 tccatcgctg ggttatgtct tatcgaaagg gaaagatatt gaaggtatcg gtgatgaaga
      961 tctggtcaat tatatcgatg tcggggctac atattatttc aacaaaaata tgtcagcgtt
     1021 tgttgattat aaaatcaacc aactggatag cgataacaaa ttgaatatta ataatgatga
     1081 tattgtcgcg gttggcatga cctatcagtt ttaatgatta ttgccggatg tgatgcatcc
     1141 ggcacatttc actcacgccg ttaacttcac cggctcgtca cgaaaatcat ccgccggttc
     1201 cagcttcaga ttcagcgtcg tcagcagttc acgcagcttc tcgtgaaact cacgcagggt
     1261 gtgctccagt cgttcaacca cttcagtatc ttttaccgga acactcttcc agtcgcctgc
     1321 tttatcgaac agaccaaact ggtaactgta ggtaaaacgg gattcctgcg cttcaagctc
     1381 catccaccag ccccagaact cacgcacttc cggtgccggt ttcacgttga cgcatacagc
     1441 cagacaatcg aaaaagaatc gattatcttc gcacttacct tcacgaatat acgggcctag
     1501 tgcggtaaat tttttgatca atctgctctt cgggtgtcca ctcggtaacg tcattgctat
     1561 ctcctgttgt gatgcaactg ttttaccaaa ttggcaaaat ttagcaaatt tttaacacaa
     1621 gcgtttttcg atccagtcgg tgatttcctg aagacctttg tcaaaattcc gatacaccgg
     1681 gttaaatggg atctctaata atttaccgtc agcagatgat gaggtgatta agcgtgagtc
     1741 ctcttccggg ctgaacggat cgttcttcca gtagcctgat aacattggcg ttgggcagcg
     1801 acgtcccagc aatccttgca cttttaatga atagcgattc agctccacgc gcaacgcttc
     1861 atcggaagca tcatgcatcc ccaaacgact ggccagaacg tcaagataca tttccggcac
     1921 ctgttgctgg cacttaaaat cactcaacag ggtatgaact accggaccaa gacaggcaac
     1981 cgctttcaga cgcggcgatt caaggtatgc cagacgcacg gcaacgttag cgccgaaacg
     2041 gaaaccaaag gccgcgacgc gagtgtgatc cacccacggt acgttaggca gcgcctttaa
     2101 gacgtgctga tgcaacaggc tggagtcctg ggtgagcttc cattttgaag aaaagcccac
     2161 cgacggcata tcaatagtca gcatcgcaat gccgcgcggc gcaaaataac gttcatacag
     2221 gctgtaatag tccgtctgca tcgcatccag accaccacac attaataccg tcgggaacgg
     2281 gccatcgcct ttcggcatat gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa
     2341 ctccatctgc cgcatcgtgc ccggcagacg ctgagcggcc tcttcatagg cgcggtttga
     2401 caaagcctgc gcttgctcgg ccaggtcatc tcctttcaga tgaggatagg cggcaatgtt
     2461 gtacaacgta gccgcatgca gccagtgacg cccactgagt tgcggatctt cttccgcaca
     2521 ggctttttgt tgccacacca tcgcctgggt ggcccactca taaatccagt tgccaccacg
     2581 gtagccaatc accgtatcgt ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat
     2641 acgcgcctgg acgtcgagga tttcacgtgg gtcaatgccg cgccagatcc acatcagacg
     2701 gttaatcatg cgataccagt gagggatggt tttaccatca agggccgact gcacaggcgg
     2761 ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg
     2821 tttgaacagg gtttcgctca ggtttgcctg tgtcatggat gcagcctcca gaatacttac
     2881 tggaaactat tgtaacccgc ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt
     2941 gaaaagatta gcgaccggag attggcggga cgaatacgac gcccatatcc cacggctgtt
     3001 cgatccaggt atcttgcggg atatcaacaa cataatcatc aaccagcgga cgaccagccg
     3061 gttttgcaaa gatggtgaca aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag
     3121 taccaccggt atccaccagg tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc
     3181 gtttcagcac tttaagctcg cgctggttgt cgtgatcgta gctggaaata caaacggtat
     3241 cgacatgacg aatacccagt tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc
     3301 ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg agtttacgtg
     3361 catggatctg caacatgtcc caggtgacga tgtatttttc gctcatgtga agtgtcccag
     3421 cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg agattataga
     3481 gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac gcactgcctc
     3541 tccctgacgc gggataaagt ggtattctca aacatatctc gcaagcctgt cttgtgttga
     3601 caacattttc tgctaaccct gtgacctgca atactgtttt gcgggtgatc gacaaggaga
     3661 cttaacgtgt ctgaactgtc tcaattatct ccacagccgc tgtgggatat ttttgccaaa
     3721 atctgttcta ttcctcaccc gtcctatcat gaagagcaac tcgctgaata cattgttggt
     3781 tgggcaaaag agaaaggttt ccatgtcgaa cgcgatcagg taggtaatat cctgattcgt
     3841 aaacctgcta cagcaggtat ggaaaatcgt aaaccggtcg tcttgcaggc ccacctcgat
     3901 atggtgccgc agaaaaataa cgacaccgtg catgacttca cgaaagatcc tatccagcct
     3961 tatattgatg gcgaatgggt taaagcgcgc ggcaccacgc tgggtgcgga taacggcatt
     4021 ggtatggcct ctgcactggc ggttctggct gacgaaaacg tggttcacgg tccgctggaa
     4081 gtgctgctga ccatgaccga agaagccggt atggacggtg cttttggctt acagagcaac
     4141 tggttgcagg ctgatattct gattaacacc gactccgaag aagaaggtga aatctacatg
     4201 ggttgcgcgg ggggtatcga cttcacctcc aacctgcatt tagatcgtga agaggttcca
     4261 gctggttttg aaaccttcaa gttaacctta aaaggtctga aaggcggtca ctccggcggt
     4321 gaaatccacg ttggcctcgg taatgccaac aaactgctgg tgcgcttcct ggcgggtcat
     4381 gcagaagaac tggatctgcg tcttattgat ttcaacggcg gcacactgcg taacgccatc
     4441 ccgcgtgaag cctttgcgac cattgctgtc gcagctgata aagtcgacgc cctgaaatct
     4501 ctggtgaata cctatcagga gatcctgaaa aacgagctgg cagagaaaga gaaaaatctg
     4561 gccttgttgc tggactctgt agcgaacgat aaagctgccc tgattgcgaa atctcgcgat
     4621 acctttattc gtctgctgaa cgccaccccg aacggtgtga tccgcaattc cgacgtggca
     4681 aaaggtgtgg tcgaaacctc cctgaacgtc ggtgtggtga ccatgactga caataacgta
     4741 gaaattcact gcctgatccg ttctctgatc gacagcggta aagactacgt ggtgagcatg
     4801 ctggattcgc tgggtaaact ggctggcgcg aaaaccgaag caaaaggcgc atatcctggc
     4861 tggcagccgg acgctaattc tccggtgatg catctggtac gtgaaaccta tcagcgcctg
     4921 ttcaacaaga cgccgaacat ccagattatc cacgcgggcc tggaatgtgg tctgttcaaa
     4981 aaaccgtatc cggaaatgga catggtttct atcgggccaa ctatcaccgg tccacactct
     5041 ccggatgagc aagttcacat cgaaagcgta ggtcattact ggacactgct gactgaactg
     5101 ctgaaagaaa ttccgacgaa gtaattattt gatttgctgc cggatggcgt tcaatcacct
     5161 tccggcagtt tcatccttca ttatccttcg ataaaagcca tccctgtaaa tgtccgtcgc
     5221 gggttgccac gttcaatctg gtgatggaac attcgccgct gtgatttcag cgccgcgcta
     5281 ttttcctgtt gctgctgctc cagcctccag gcaatcagca atcgcgccag ccgtttgtta
     5341 gcatgctggc tgcgctctga ctgaaccttc acactaatac cggatgccag atgcgtggcg
     5401 cgtaccgccg agtcggtttt attgacatgt tgaccgcccg gccccgacga acgcagcgtc
     5461 tcataacgga ccgcgtcgga ttgctcctgc tcatcagcag taaaacgccc aatgcccaga
     5521 aaccagtttt tgcgtccatg atgaggccga tacggactcg gacaaatcca ctgaatagtg
     5581 ccgcaccaac tttcgcttaa cgcccatgcg ttgtcgccat ccagagaaat cagcgccgaa
     5641 cgcagcgtgt cagagtagcg gcccgtttct gtttccagca ccgttaccgc gacgtcttgt
     5701 cgggtaaaaa cgaccaacag agaagaacgc tgcgccaatc gggtagccgc gtccggggtg
     5761 taaatctggc atccccacta cgcgctgcat gttgggtaaa tttgccgtgg tgtgtaattg
     5821 ttggatcgct aaactctcga tccacaggtc atcagatgcg atggtaaata ccgcatcgga
     5881 taagggacga atatatttgc ccatgtacca ttccatttat actcgtcata cttcaagttg
     5941 catgaactgc gactcgaatt atttagagta tataaatata aaaatcagga aatggcagac
     6001 gaatccaggg caagaaaaaa agaattaatc ctggaaaggt ctgcaaagag tcattgataa
     6061 aggatttgtc atgatttacc tccttgcagt ttgtggaata agaaaacgcg cacataccag
     6121 gcgggcgtta ttttcattgc aagctggatt taatgttgcg gtttatatcg catataaaaa
     6181 ttagtaaacc acgctccccg gcattcaacg cgctgctgct taactgtctg aaaaccataa
     6241 cgttcaaaaa agggttttgc ggttatgctg gcgtccaccg taagttcgga ttcagactta
     6301 atccaaggtt ttaacaaagc actggcaacc ccacggcggg tgtattcagg gtcaacaaat
     6361 aacatatcga tataatgttc aatgcgggaa ataaaaccaa ccggttgtgc attaatgact
     6421 gcaacccgca cttgtgattt cgcgagtttc tccttccagc gagattcgtc aatctgcgcc
     6481 caggcggcaa tttgttgtgg tgaataatgc tgactggcgg tcatcataac cgctctaatg
     6541 aaaatagcgc atagttgctg aaaatcgcca ggctgatagt ttcttatttg tatgttattc
     6601 ataatcccag caccagttgt ctttccattt gcgggtcaag caacgtcaca tgcagcccca
     6661 ccagtcgcac accgcgcccg ccgcggcgtt catcccaggt tttacgcgcg gtggcgatta
     6721 agtcagcttt attcagccgc ggccagacgt gctcctgggt ggtttgctga aaatcgtcga
     6781 actttaattt caccccctgg cgagcaatca gtaaatcagg ttttaccttt gccagacggc
     6841 gttcaagttc cggatacagc cgctcgataa tcgcttcaca ttcagaccag tgatgaatat
     6901 cttccgccat cgtgcgttcc acgccgacgg attttcgcaa ccgttcgctg ttaacgtcgc
     6961 gctcgtcaat cccctgacta cgctcccaca aaatgcggcc aaatttgcca aagcgtttaa
     7021 gcagcatcac cagatcacac ttttgtacat caccgcaggt ccgcagcccc atcgcttcca
     7081 gttttgccgc cgagactttg ccgacgccgg ggatttttgc cagtggtaag gtttgtaaaa
     7141 atgccggaac ttctgccggc gtaatcacaa actggccgtt gggtttattc atgtcggagg
     7201 cgattttggc gagaaacttt actggcgcca cgcccgcaga cgccgtcagt tgcagctcgc
     7261 tgaaaatcgt ctggcggatt tcctgggcga tgagggtcgc agaaccgtgg caatggacgc
     7321 tgtcggtgac atcgagataa gcctcatcca gtgacaacgg ctcaatgcga gaggtgtagc
     7381 gcgagaagat ttcacggata tgatttgagg cttctttgta ggcgtcaaag cgccccggaa
     7441 gcaaggtgag atgtgggcat aatttgagcg ccatccctgt cggcatagcg ctgcgtacgc
     7501 caaatttacg cgcgggataa ttggcggtgc tgatcacccc ccgacgttcg cggctgccgc
     7561 caatagcaat agggatatcg cgcagggcgg gattgtcgcg catctccact gcggcgaaaa
     7621 agcagtccat atccacatga atgattttac gcattgctca cctctcaaca ctggtaaagt
     7681 atacagtgat ttcagggttt gagaaatgcg taaagattca gcatttagcg cgttcgctgc
     7741 gaaagcacct gctgcttatc cagcttttgc accagcggct gcaccacttt atccccatgc
     7801 tgaccaaagt attgatacag cgtatcggac gcacttttgg tcagcaccat aatctcaata
     7861 cgccgattgc ccgcgctttg cggatttttg gcatccagca gcatctggtc cgccattgcg
     7921 cttacctgca tcactttatc ttccggcatt ccggcctctt ccagcacccg acgagctgaa
     7981 agcgcgcgat cgcccgaaag gttccagttg ttgtagatat tgtttttgta ggccatcgca
     8041 tcggtatgcc cggtaataat aattttgtta tcgagcgagt cgaacactgg cgcgagctcc
     8101 accagcaaag ttttaaagaa cggcataatc tgcgcgctgc cgcgttcgaa catattgcga
     8161 ttctggtcgt ctttaatcag cacgcgtaat ccctgcggga caatctccat ctccagattc
     8221 gcttccatat gcgcatcacg ggcaatggtg ttgatactgg ttgccagctc gccaagctcg
     8281 gtggcggatt tcttcttcag cagcgccgtt ttctcattca catcacgcgc ttttttctcc
     8341 gtctcttccg gcaccgccac ggtggcaggt tttggcggat gagacgggct gattttattt
     8401 aacggcgaca gcccaccacc gttaaaaatc gactgcccgt gcagcgcggc gataatgctt
     8461 tcacgctcgg atttactgac gctgttgaca atccacagcg tcataaacag cgccatcatt
     8521 gccaaagtaa agtcggcgaa cgccaccttc cacgccccgc cgtggtgccc ggcatgattc
     8581 tttttgatct gccgccggac gatggtggtt tttgccccac gctcgcggcg gttgcccgtc
     8641 tttctcatgc ttattcttgc tccagcatcg cattcaccca cgcgtcgagg ttggcgaagg
     8701 tcggtttaga tgccagatgc agcagcttac gcccggcatc caccgccagc agcgtcggtt
     8761 tgccgcctgc ctgcgccacc agcacggtac gcacgcattc cagcaaagaa tgttctgccc
     8821 gcgcctgttg ttccatggcg ttggcgagcg gatccatcag gcagtagcag ataaacacgc
     8881 ctaaaaacgt ccccaccagc gctgccgcca ctttcaggcc aatcagggcg atagagccgt
     8941 caatcgactg catggtgatg atgatgccca acaccgccgc acaaatgcca aaccccggca
     9001 tcgcttcggc ggtacgttgc agcgagcgcg acggcgttag cagcgactct tccgcggtgt
     9061 ccagttcctg atcgagaatg ccttccagtt catgggcgtc gattttaccc atcgccatca
     9121 ggcgaaagtt atcggcaata aaggtcacca gccgcttctg ggttaacacc agcgggtatt
     9181 tctggaaaat ggtgctctct tccggttgct caatatgttg atccagcatg cgcagaccgc
     9241 cgttttgcac catttcgagc aactcataca ggcacattaa tagctgacgc tgaaactccg
     9301 gcccgagctg ttttttactg atcacacctt taatttgatg ggcaatctct ttcagcacat
     9361 gtttcgggtt accaataatc atcgccccga aaccggcacc taaaataatg atcatctccg
     9421 ccggttgcca gatagccact aattgcccgc ctgcttcaaa ataaccgcca aatacgcagc
     9481 cgagcaccac cagtaaacct aatatttttt gcatgatttc ttcactttaa ttcggataga
     9541 caaaatcacg aattttctgc gcgatctgtt tattcagttg acaaattcgg gcctcggtta
     9601 gccctaatac cagcgcaatc tctttgagat tcatctcatg ctggtaatac atggagagga
     9661 tcagttgctc tttttcgctt aattgtgaaa gcgcgtgttg caacatttgc tgggtgacaa
     9721 actgatcttc cagttcccgg ccagcaagcg gaatgcctgg cccttcgccg ttcagcagtt
     9781 cgtcaaggct ggcgagcgtt tccgcccctt ccagttgctg atattcgaga taatcttcat
     9841 acgagatccc ttccacgacc agttctggcc actccggttc atgccccagt tttttgcgcg
     9901 tctcacgaat cagatctttc atctgatgat atttctgccg cagctggcgc ggtcgccagt
     9961 cgagggcgcg caactcatcc agaatcgcgc cacgaatacg atgcacggca tagcccgcaa
    10021 agccttcgtc cggcagcccg tagcggcgaa tggcattaag cagacccatt aacgccgttt
    10081 gttccatatc ctgacggtca ataatgcaat tacactgcgg ggcaagttgc cgcaccactt
    10141 tacgtaccag cggtaaatag gcctgcaagt agtggctctc ctgctgcggg gttaatactg
    10201 gcgcggcagc aaattcttct gactcgaata catcctgtag catgtttata tccgcctggt
    10261 gattactgga agaccacttt tttcaccagc aaatccttat acggtgcgct cattttgcga
    10321 cgctgaagat ctttctttaa cgcgtcagaa atgagtttgc gtatggcaga tattttcatg
    10381 ccgcgcaaat cttcaaattt catttccgtc aggcattcaa ccgcaatact ttgatacagc
    10441 ggctcctgat gttttatttt ttccgcttct ttgtcactgg cgaccaccat cactaattcc
    10501 gccgacaaat aatggtccgc gccgttgttg tcatgcagcg tgacaatggt ttccggcaac
    10561 gtcacaaaca cgcttttgct ttcatccagg gtttccaccg tttccgtctg cgcggcggca
    10621 gtcggttttc cttttccggc ataatgatgc cagacacccc atccagctcc ggcccccacc
    10681 accaacgcca gaacggcgct gaccacgccc gccaccacga ttttcttcat attgccttta
    10741 cactttgatc aaaacggatt cgttctgttg aaattgaggc gcatcatcaa gctgtaaggc
    10801 ggcactcacc tcttcctgct gatggcccga ctgttgttgt tgctgcgact gcccttcgga
    10861 ggaaacctgt acgttcacct ccataaaatt ttgcgccatc agatgctggc ggagatcgtc
    10921 gctaaactgc tgtaacgcgc ggcaaacttc actttggttc gcgccgatgt gcaccatcag
    10981 tttgacgttg tcgagttgca cggcgatctc gagcttgcca agcgacggtg gatcgagacg
    11041 gatggtggag atctgctgtt gtttgttgag ctgaaagtgg atctgatctt tcaaaagcgt
    11101 cgtcagtttt tcgcccagtt cgtccatcga aagcgtggct ggtttggcga caatctcctg
    11161 atgatcgctg agcagctccg gcgtcttaac ctgcaacacc gcacgcgcaa taaccggttc
    11221 gctgtccggc actttttccg ttttacgcac cactggaggg cgcacgatca cgcgggtcgg
    11281 ctcacttttc gcgcgttctg gtttcgcggg cgcacgctgt tgcgtcggcg caatggcatg
    11341 taaatcttca ctggcgtaag ttgccagttt ggcctgctga tccggtttcg cctgcggcaa
    11401 ctgactgatc agttcctgca actgcggcgg caacggcgtg agttcctgct gttgcgtctg
    11461 gcgttgcggc gcgttttgca ccaccgcttt cgtcaattgc tggataaccg gcgatgttgc
    11521 gacgtttcgc ggctgcggcg tgtcctgatg cggcgcggca ggttgtggaa gtaacagcgc
    11581 cattagcgcc tgcatcgcca ccggatcgtt ctcctgcggg cacgtcgcct gttgctgact
    11641 ggttttagcg ctatgaacgc gctccgccac cgccgcgacc gccattttcg gcagggtaaa
    11701 cgcgggcgcg ttttcaccgc tcaccggcgg taaaatatcc gccttcaacg aagcggtttc
    11761 cgcaagcgtt ccgagcgtgg caagcaatgc cggattcatg acttctccga aagtaaatta
    11821 acaattgagt aggccagtac gccttcctga tgccgctgat gctgttccat acgcgctttc
    11881 aacacttctg cgcgctgctc gcgccgttta atcagggtat taaacgcgtc atacagccgc
    11941 gccttcaccg catccaactg cgccgcgtcg gtttccgccg ccgcgtaatc gtgaaactgg
    12001 cgcatcaact gctgatgcag tgccggaagg cgtcgccagc gttgcttgtc gagggcttca
    12061 ttcatcgcct cgaccaatgc gaaaagttgc ctgcgttgtt tttccatcgt cttaacccag
    12121 cttgcccgaa agcccttccc agccttcgcg caggttgctt aagatcagca tcacttcatc
    12181 gattttctct gccgacaatt cgccgctggc ttcatacaat cggtaaacgc agtggtcata
    12241 gaggcgcgaa aggttgacca ccagctcgcc gccagtttca aactccagcg aactggtgag
    12301 cgcattgaga atgtcgatgc atttattgat gctctgggct ttcttctcga aacgccgccc
    12361 ttcaatatgg cttttggcgc gttccagctc atccatcagg ccagaaaaca gcaccagcac
    12421 cagttccaga ggcgatgccg ccgcagtacg cgccgcgaga tcgatctctt tatattgcga
    12481 atagccgtcc tgtacttggt acatctttag ggatattcct tctgagttac gcgaatgccg
    12541 ccatgctggc tttcatggtg tatacctcga ccagcgtgtt ggtgtactcc tcgagataac
    12601 gctcgtagtt ggcgttatag gtatcagtaa gctgatcgcc ttcgtcctga attttgctca
    12661 tctgatcgtc aatgttctgc tggcgcaggg taatgatgcc gttgctggag tccgtgtagg
    12721 tgttaatcag gtcgtccatc tgcgccacca tgctgttgtc gccaacgaaa atagaggtca
    12781 gaccgtcagg atttttcgcc atttcgtcgt tgaactggtc ggagtcaatc tgtaagtggc
    12841 cgtgagaatc aagcgtgatg ccatagtcaa caatcgacac gccgttgtag ctggcatggg
    12901 cgatgtcatc gagctggttc gccagtgaac taagtccggc gtcgcctgca aataccccgg
    12961 cgctggtact gtcatcaccg tgggtggtca gcgaatcaac cgtatcaatc aaggtgttat
    13021 aggcatcgac aaaggtctgt actttctcct ggctggcgct ggaatcttcg ctgatgttaa
    13081 aggtagtgag atcgctgtca gaatcgctga cttcggtgaa agtcatggtg acgccgggga
    13141 tcacatcatc aaacgtattg ctgctgttgg tgctcgccgg gcccgtggcg ctaccgagat
    13201 gaataatcgc gtcctgagcg gaggaaacat ccgtggcaac gggcgcgctg gtgctatcgt
    13261 tgctggtgtc atgccccgtt acgctcaccg aaaacgcgct ctgcgccccg gtgctatccg
    13321 aggtgagcat aatcgtggtg gtaccgtcgg tttttaccag cgccgccgac acgcccggat
    13381 tgtcatcgga gtcgttaatc gcattcacca gttcactagc gtcgataaaa ccgtcgccgt
    13441 caccgttctg atccgccgcc gacagatcaa tatccacggt gctgtcgccc atcgtcagtt
    13501 cgaaggtgcc ggtggcggaa aaggcgtcgt cgcccatact gaacgtggtt tgctgcccct
    13561 gcgcaagttg ctcaacgaaa aacgagtagc ttcccgcctg cgcctgcgag ttagcggaaa
    13621 cggtcgccga gtcattattg ctggtggcgg caaacgtcac cgggccgtcg gtatcgctgt
    13681 tcagcgcgtc aacggcgctc tggaaatcgc tcaacgctga gctgagcgaa tccagaccgc
    13741 tgctttcggc atccagctcc gtttgctttt cctgcaagtt tgccgcctga gtggcaacgt
    13801 cggcataggc gatttcttgc gcaatggttc ttgggttgat cattgatgac tccgggtaac
    13861 gatgtcaggt tattggcaaa aggcgtgcca atatttttta tgtgaataat caataaattg
    13921 aaaaaggggc ggaaatggct tttccggtct ggcggaaaaa agcctgccgc agaaagcaca
    13981 acaccgcccg caggcggtgt tgagactgat gacaaacgca acactgcctg atgcgctgcg
    14041 cttatcaggc ctacaaggtt tctgcaatat attgaatttg caatattttg taggccggat
    14101 aaggcgttta cgccgcatcc ggcaagtccg ccattactgc agcaggctgg aaaccatgct
    14161 ggacatgctg ttggactgtt tcagcatggt gatgccggtt tgcatcagca cctgctgttt
    14221 ggtcatgttg gacgcttccg tcgcgtagtc ggtatccatg atgttaccga tagcgacttc
    14281 ggtgttgtcc tgcatgctag tcaggttgtt ggcagtgtcg ttcagacggt taatagacgc
    14341 acccagcttg gactggatct gcgaaacatc atccatcgcg gtagaaatgc tggtgatcat
    14401 ctggttagcc gagccagacg cagtcagctc agtaccgcca gacacgcctg cagtacccgt
    14461 ggtgtctgcc tgatccgcac tgaaggagtt gctgatagca gaaagatccg tcaccagggt
    14521 attcagctgg cttgaaatat tcacggtcat ggtgtcggaa gattccgcgc ccacctggaa
    14581 agtcaccgcg ctctggaaca gaccgtcagt accggtgttg ctggtgccgg aaacaccgaa
    14641 caggttggtg ccgccgtaag tggtgttttg cagcatgtca gacatctgct gacccagttc
    14701 gtcgtattcg tcctgcatcg cctgcagatc gtcgtcgctg taggtgccgt tcgctgcctg
    14761 agttgacagg tctttcatac gccccagcac gtcagacatt tcgtcgaaca tgctgtcagc
    14821 ggtttgcagc atcgcggtgg catcattgat gttgctcagc gccacaccca taccgctgga
    14881 ctgtgctgtc agacggttag caatttgttt gcccgctgcg tcatcggcag aagagttaat
    14941 ccggttacct gtcgccaggc gttccataga tgtagacaga gaagagctgc ttttgctgat
    15001 tgcattaacg gcggccattg aggcgttatt ggtgttaata gataacatga tactgctcct
    15061 tgggattaat cgttttcgtt tcgataccct gtaaaaacga cacatcagga gcaaacttaa
    15121 aatcgctatg aaaaaatttt cgctacgtat tatctgatgc cacttttagc taaaaaaagc
    15181 agagtttaaa ttcagataaa aagattatta gcaatatctg ccacatggcc aggtatcaaa
    15241 atatgtcttt atcaaactct tgatatagtt aaggttatca tcaattaacg cgttttttcc
    15301 tcaatctact gatgctaaaa tttacattgc gttgcaaata gtacatattt ggctattgta
    15361 cctgtcgtgt catttcgtta catttccacg ccatatagtg attaagaata atcctattgc
    15421 cataaaatat taattatcac gcagaaaaat catcttctca gggacgtata agactgtgct
    15481 ttatgcaaag cactatcagg aaacatttat cccttgctaa ggtaaactca actatgagta
    15541 ttattatcaa caactggcgg atggacccgt cgcttaatgc cttaatacat tgcgaaacgg
    15601 gtgaaacacg tcgtctcggc gaataccatt ttattttgct ggaaacatta gcaaaaaatg
    15661 ccgatgtagt tttatcgcga tcttttttat gtgccgaagt atggaagaac cgcattgtcg
    15721 gcggtaacag cctgccgacg gcgatccatg cgttgcgcgt tgctatcgac gatgatggca
    15781 aacaacagaa cattattaaa acaattccga agaaaggtta cttatgcaat aaagagtatg
    15841 tgtctttgcc ggagtcatcg cctgcagaag cgctgattat taccgaccag ataaaggaaa
    15901 cagtgccaga agagatatct tcgacaacac cgcctgtacc tgtcagaaaa aaacataaag
    15961 gactgatggg cttagcgttg accgcggctg ttatatttat tggctcgacc gtggggtatt
    16021 cacacttgaa aagtacacct gacgccccgc aactggtaaa agagtcaatt aacagtccaa
    16081 gaataaaaat atttcatcta acttctgctg gagaaaataa ttctgcaccg ttactttcac
    16141 aaaccctcgc accagggaaa gacaaactcg ataatttatt gtcagcgcat aatatgacaa
    16201 tgacaaccta ttataaatat gttcgtaacc gactggaaag tgatattgtt ctgcgtaacc
    16261 agtgcaatgg tagctggcaa ttaaccttta atgtggatag ctggcagaat agtgatatta
    16321 atagcgcgat gtatcagaat ttagagaagt tattaaatac tgtgcagaaa tgctgatgag
    16381 cgctaataaa tgccggatgc gacgctaaac gcgtcttatc cggcctacga aaattccgaa
    16441 tgaaatcaat cggaattact gatagttgta gttattggta actctaatct tttattaatt
    16501 atagtttgcc ggattcgttc tgctttttta attattcgcc tacgactttc ggggctttgc
    16561 ttatccatca gttgcttgag agtggccatt tcattccttt ttaacccgca gatttatagc
    16621 atattctgca ctgaaatgat tttttgcgac agcctcattt gtttgatttt caataaccct
    16681 accccaataa attcctcacc gtctggctgg aaattcgcgc ctggccgttc accgcctgca
    16741 ataacacctg atagttatga ttggcgctgt ccagcacgcc gccgaggttt tccgacgcct
    16801 gcaccgcgtt ttccgtttgc gggaagcgcg acatgccgtc gatcaactgc cgtgcttttt
    16861 cctgctgcac tgccatttgc gcgcgctgct gattaatgtt ttctagtatt tgagaaatcc
    16921 ccgccccgcc ttgttgcagg ctttgtttta accgctcggc ctcagagggt tcggcaaaag
    16981 tttttagcgg cataaacgcc gacttatcgc catcggtgcg cacgcttaag gttcgctcga
    17041 tatcaggcca ctgtttttcc gtggtggaga aactgatgcc atccgcttgc tgatgcaact
    17101 gaacgcccac gcggcgcagg gcgttggtca gccgcgtctg atactgcccg gcgctgtcat
    17161 cctcagacaa catcaccgcc gaaagctggg tctgccgccc gtcagagacg ctgaacatgc
    17221 gcgttcccgg agtagggttg tgtatcagat tcgccagatc cggcgagtga aaggtcaccc
    17281 gcgcttcgcc ctgcaacact ggctgcaact ggcggtccac cgcaccgccg gaaagcgcgg
    17341 tgcgtttgtc cagcatctgc atcagcgccg tattctgcgc ctgcccgcct ttacgctggc
    17401 tgtgacgata gtcgagcaac tgctgctcca gttgcccgag gtagtgatcc gcctgctgca
    17461 acgtggtgag ctgatcgtta agctgcacgc tgtaacgctg cgggcgagtg gtaatcagcg
    17521 gcgaggccgg atattcactc gccgtcaccg ggagtttttg ccgcaccgcc tgagtgggcg
    17581 caacggttga cgacgatacc gcggcgctat gcgcgccgcc ggtggcgagt gacgaagaag
    17641 taagtccgac ctgcatactg ttcctgaacg agttagagaa cgctgaacaa attcaactca
    17701 ctcacttttg agtaagtctt gagggtagct tccattgcca cttccagtcc ggtaaaggtg
    17761 atcgacgccg ggccgtaatc gagatcctgc aatgaaccga tcaactcatc attagaagta
    17821 gagatatcgg tctgtgcatc gctcagcatc gacatggtgt tctgcgtctc gccgagcgac
    17881 gcaatcgagg cgttgaggtc gtccgaggcg gtgtccacca catcaacggc gttctgaata
    17941 tcgctctgca cctgcggatc tgccgggtcg acgtccgggt tttgcaactc ctgggacagg
    18001 gaattcaggg tgttgagcac gtctaaattg ctaccgaaga aatcgctcgc cgccacgttg
    18061 gtatccactt cgacgccgtt cgacacggtg gtctggcggt ggtcgctgtt accctgatag
    18121 ctgtagctgt cggtcacgcc gtcgccgtca tcgtccaccg ccacaatcgg cggccgatcg
    18181 ttgatagtac cgccaaacac gtagtgcccg tcttcatcct gataattgag cgctgcgacc
    18241 atcgattcgg tgagcgactc aatatcctgg ccgaggcttg cgagcgagtc ggcggtattg
    18301 gtgccgttcg ccgcttccag caggtcgtcg cgcactgcca gcaagctgtt gttgacgcca
    18361 tcaaggattg attcctgctg gcttaagccc gccgacgcag aatcgatatt gctctgatac
    18421 tgttcaatcg ccgactgctg gcggtttaac tgggtgatgc gcgtggcggc gatcgggtca
    18481 tccgacggct gcaaaatttc cttgccagtt gccatctgct cgaccacatg cgccagatcg
    18541 ccggagagat tattaaaact ttgcgtcatc gagacgtagg tttgttgggt ggttactcgc
    18601 attttcgcgc tcccgcttag ctgcacattt ccagcaccga atcgaaaatc tcggccccgg
    18661 cggaaatgac tttcagattg gcttcataaa tttgttgata ggtgatcagg ttcaccgcct
    18721 cttcgtccat gctgacgccg ctgacgctgc tctgctggtt ttgcgcttca gaatagacat
    18781 tggacgcagc atcgacttcc gtttgattct gctgactgta aatgccgata ttgctgatga
    18841 ttgacgagca cgcctgcccg accgtcacgc tgcccagatt gtcaatctcc agcggttcgg
    18901 tggagatatt gatcagcgcc tggaggttgt cgctgttccc gctttcgtcc ggcgaactgg
    18961 agaacgccaa ctcgtcggcg gtgatatccg ggttaacggt cagcgggcca tcggcgttgc
    19021 tggcgtcata gataaacagc ggctcaccgg gattaccgtt gagatcgtag ccctgcgcaa
    19081 gctggttgtt gaccgcatcg gcaaactgcg acgccatgct gttgatggtg tcggtcagcg
    19141 gcgtcaggac gtcgttttga taatcaaaca gtgcgcctaa tgaaccgccg gtgtcggtgg
    19201 tcatcgtcga ggtggtgcca gcgaaagtca gcgacatggt cggcgtgccg tcggcgttgg
    19261 tttccagcgc gatggtcgag ctttgctgcc cgctcaccag cggctgaccg tttttcaggg
    19321 tgacgttgta gttgccctgg tcgtcgatat taacctgcac atccatcatc ccgctgagtt
    19381 cttccaccat ctgatcgcga gcgtcgtaca gcgcggaggc gttatcgccg ttggcttcgg
    19441 cctgcgcgat ttgctggtta tagctggcga tgccgctggt gagggtattg atttgcgaca
    19501 ccatcgcctg ctgctggctg atgatttccg tactttgcga gtcgatgtaa tccagcgtgt
    19561 tatcgatacg caacgacagc gccccggctt cgctgatcac ctgctggcgc agggcggaat
    19621 catcggggct ggtggttgct tcgttgaggg cggcgaagaa gttatcgaac ccgccgctca
    19681 ggctgctgtt atcgtcgctc aataccgctt ccagctggct gagataccct tgctgggtgc
    19741 tgtaatagcc gtaatcgctg gcggcatacc acacctgatt cacctgatac tggttagaga
    19801 cgcggcgaat gctgtcgacc tgcacgccgt taccggcgct gttcggcgaa ccgccgctgg
    19861 caccgatggt gctgatctcg gccacctgac gggtgtagcc cgtggtcatg gcgttagcgg
    19921 tgttttgcgc cgtgacgttc agctccacct gcgcggttga tgcgccgctg tagccgatgt
    19981 taatcatgtc catgttggat ccttaccggt aaatcgtgcc ggttaaaagc ggcgtgaagt
    20041 tggggattct taagttccat aaaccgggtg ccaaatgccg gatgcggcgt gaacgcctta
    20101 tccggcccac atacgggcga ctcgtaggcc tgataagacg cgacagcgtc gcatcaggca
    20161 ccgtgcacca aatgccggat gcggcgtaaa cgccttatcc ggcctacata cgggcgattc
    20221 gtaggcctga taagacgcgt cagcgtcgca tcaggcaccg tgcaccaaat gccggatgcg
    20281 gcgtgaacgc cttatccggc ctacatacgg gcgactcgta ggcctgataa gacgcgacag
    20341 cgtcgcatca ggcaccgtgc accaaatgcc ggatgcggcg tgaacgcctt atccggccta
    20401 catacgggcg actcgtaggc ctgataagac gcgtcagcgt cgcatcaggc accgtgcgcc
    20461 gaacgccgga acctcattta tgcttcggcg acaactgctt cacaatcatc gccgcaatcc
    20521 cgatcccatg ctgctgcgcc agggtgttac acaattcatc gtcataaaac ccctgcatca
    20581 tgcgcaccga atcgctgtta aacgggttat ctttcgaatc aaacaactcg ttggttttac
    20641 gcatctcttt cagcatgttg cgtaaaaaaa tcgcctcaaa ctgttcggcg gcctgcttaa
    20701 tatcattggc ccgaaccttc ggccccatta acgcatcgct gccgtcaatc ccgccgctgg
    20761 cgttaacttt catcagatca cctccaggtc ggcatccagc gcgcccgctt catgcagcgc
    20821 ctgcaaaatc gacataatgt cgtccggcgt cgcacctaaa ctgttcaacg cattgaccag
    20881 cgttttcagg ctgctcgact ccggcaaact caccacgcca gggcgcgcat gattcaccgc
    20941 gatattgctc tgcggcgtta ccgccgtgcg cccgcccgca aaggcattcg gctggctgac
    21001 gttgctggtt tcgttaatcg agacagtcag gctgccgtgc gacaccgccg ccgcatgcag
    21061 cgcaacgcca tcgcccatca ccaccgtgcc ggtgcgcgag ttgaacacca cgcgggcgcg
    21121 gattttctcc gcctgcacct gtacatcgtc cagttgcgac ataaacgcca cccgcgcgcc
    21181 ggggctggtg ggcgcgcgca cggtaacgtt ggtggaactc tgcgcggtgg caatgccgcc
    21241 aaacgaggcg ttaatcgccg ccgcaatgtt gttagcatct ttaaacgacg gacgtttcag
    21301 gttgagcgtg atggtgtcgc ccatttgaaa atcgctgggg atctcgcgct ccaccgttgc
    21361 accgttggga attaacccgg cggtcggcgt attaaccgtc acgctggaac cgctggcccc
    21421 ggtggcgttc atcccgccca ctaccacgct gccctgcgcc agggcataca cttcgccatc
    21481 tgcgccgtgc agttgggtca gcagcaaggt gccgccgcga atacttttgg catcgccaat
    21541 cgacgaaacg gtgacatcaa tggtctgccc gcgcgagtac atcggcggca atgtggcgct
    21601 caccgccact gctgcgacgt ttttcacctt cgggtcgatt ttattcggca actgcacgcc
    21661 aaactggcgc agcatattgg tgatggtctg gttagtgaat ttgacctggt ttttatcgcc
    21721 ggtaccgtcg aggccaacca ccaggctgta gccgaccagt tggttttcac gcacgccttg
    21781 tacgttgacc agcgactcca gcgattgcgc cagcgccacg ccgggtaacg ccaggcttac
    21841 ggcgataact gcggccttta tccagttttg cattgcactc tccggcatta aatcgggaac
    21901 agaggatggt taaacagtcg cgtcagccag cctgcggcgt tagcgtcgct taacgcgccg
    21961 cgtccggcat aggagatgcg ggcatcggcg atccgctgag aggaaaccga gttgtcgttc
    22021 tgaatatcgt cggcgcgcac aaggccgctt aagcggatgt actcatcgcc ctggttgagg
    22081 gtgagccatt tctcgccgcg aatttccagg ataccgttgg gctgcaccgc atggacggac
    22141 acggtgatct ccccgcgcag actgttctgc tgctgggagg tggcgctgcc gtcaaagtca
    22201 cggttggcat cgatggagcc ggaaagctta tctttggtgt gaccaaaaat cgtcggcgca
    22261 ccgatatcga cggtgttgtt tttgccgaag ttggttttcg cctgtttgct cgactgggtg
    22321 gattcttcga ggatcaccgt caggatatcg cccacgcgat aagcccgccg gtcggcggtt
    22381 agcgaccagt tgtagccggt ttcaaataca ccgccagcac gcccgtctgc cggaggcggc
    22441 gcttcggttt tcggtggcgc aaagtatgcg tcgtcttttt tgaccagaat ggcctgcgat
    22501 tcacacccgc taaggagtgg aagcagcgcc accagccaga gataattttt cacgttctgc
    22561 ctgtttagtg ttgcaggcca cgggagtggc cggttgtttg gtgggtgagg agcggtttgt
    22621 ttcccctcac cctagccctc tccctcaagg gagaggggac tgacctgtgc gcgttgtaac
    22681 atcgtgcact gcttttctcc ctctccctgt gggagagggc cggggtgagg gcatcagcgc
    22741 gcatgttccc cctcacccta accctctccc tcaagggaga ggggactgac ctgtgcgcgt
    22801 tgttacatcg tgcactgctt ttctccctct ccctgtggga gagggccggg gtgagggcat
    22861 cagcgcgcac attgcccctc ccttacagcg tctggctgat gtactgcaac atatcgtccg
    22921 ccgccgacac cattttggcg ttcatctcgt acgcgcgttg caccgtgatc atcgccacca
    22981 tttcgttgac gatatccacg ttcgagcctt ccagcgcgtt atcctgcaag gtgccaagcc
    23041 catcttcgcc cggtacgcct tcggtcggct gaccgctggc ggcggtttcc agatagaggt
    23101 tgtcgccctc ggcggaaagc cctgccgggt tagcgaagtt caccagcgtc agttgcccca
    23161 gttccgtggg gtcgctgtcg ccgggtaaga tcgccgtcac cgtgccgtcc tcgccaaacg
    23221 caacattagt cgcccccgcc ggaacgtcga tttccggctg caacggcaag ccctgcgagt
    23281 tggtcagcac gccgtcggcg ttcacctgca aattgccgtc gcgggtatag gcaatatcgc
    23341 cattagcctt ttgcacctgc aaaaatccct gccccataat ggcgacattc atcgcgttgt
    23401 cggtggtttc aacgttgcct tcggtgaagg ttttctgggt gccgacaatg cgcacgccgc
    23461 taccgaattg cagcccggtc ggcatgatgt tgttctggtc gagcatcgcg cccggcgctt
    23521 cctgggtctg gtagaacaga tcctgaaaca tcacccggtc gcgcttgaag ccggtggtgt
    23581 taacgttggc gatgttgttg gcaatcgcgc tcatttcagc atcctgcgcc gaaaggccgg
    23641 ttttgctgat ccataatgct gcgttcatgg tggtaaattc cgtgttattt ttgggccgcg
    23701 gttgccggat gcggcgtgaa cgccttatcc ggcctacgat ttgggcacag gcttgtaggc
    23761 ctgataagac gcaaagcgtc gcatcaggcg ccaccgcata actgccggat gcggcgcgca
    23821 cgccttatcc ggcctacgat gtgcggttac gagccgcgca ataagcggtt gcccgcgtcg
    23881 ctgatatctt cggcggtttt catcatcttg atctgcgctt cgaactggcg gttcatagcg
    23941 atggaagaca tcatctcgct caccgccgag acgttgctgc cctccagaaa accgccgctg
    24001 actttgatgt tttcatcgcg ctgggccgga acgccgtcgc cagtcaccag catcccttca
    24061 ggatttttcg ccaggttagc gaccggaata tccaccagct tcaggcggtc gatatccatc
    24121 gtcgcggtta cgtcgccgtc atcgggcgtc accgacagcg tgccgtcact gccaaacgaa
    24181 gcaatggcgt tcggcggcag aatgatcggc ccgttatccc ccagcaccag attgccgtcg
    24241 atggtcagtt gcccctggtc gtcctgctga atgttgccgt tgcgggtata cacttcgtta
    24301 ccgtttttat cctgcactgc aatgtaaccc gcgccctgaa tcgccacgtc gagcgggcgt
    24361 tcggttttct ccgctacacc cgtgctgtcg ttcacgccgc tggccccttc ctgcgccata
    24421 tagcgcgtgt cgaagccgcc acctttcacc ttgtcgttgg tcgccatcgc catatcggcg
    24481 cgaaaaccgt tggtgttgac gttcgccagg ttgttggcgc tgatctgctg ctcgtacagg
    24541 gtctgagaag caccgctcag ggcggtataa atcaaacggt ccattacatt gcctggaaca
    24601 gcgcgtcatc gagctgcgtg ctggtggcga taactttggt gttggcctga tagttacgct
    24661 gggcggtcat caggttgacc agttcgctgg tgatatcgac gttagagctt tcgagcacgc
    24721 cggacgacag cgtgccgcag gtgccggagc cgggaacgcc aatcagcggg gtgccggatt
    24781 cgccggtttg tacccacgcg gtgccgctca ccgcctccag gccgttttca ttcgggaaag
    24841 tcgccagcac cacctggccc tgcaacatgc gctcgccgtt gctgtaggtg gcgtaaactt
    24901 tgccatcgtc atcaacctgc acgccgtttt gcgttgcgga agcgtagccg ttggcggcgt
    24961 tggtggtgac cgagaagtcc gagccgtatt gcgtacaggt ggtgtaatcg acggttaagt
    25021 cgatgggtgc ggcggcgtcg gtctgaaatt caatggtctg cggtgtggtt ggcgaagtca
    25081 gcttcccggt gttcgggtcg aaggttaagg tggtcgccgg aacgccggtc tgctgctgac
    25141 cgtcgaaggt gtactgcact tcccaggtgt tgtcgctggt tttagtgaag tactggcata
    25201 ccgagtgttc gttacctaag gagtcataca ccgtggtggt gtagctgtcg gtatacgagc
    25261 tgctgttggt cgcgtcgaac ggcacggtag tgcggtcgat agcgtcgtcg ctggcgttga
    25321 agttggcggt aaaggtcagg ttgctgctgg cctgcgccgg gatattgccg gttttgatct
    25381 ggatgtccgt taccgtaccg gtttgcaggg tgcccgtgtc atcgaccgga taaccctgca
    25441 aatagtcgcc cgaggcgttg acgatatagc cgtccttgtc ggtttcaaac gatccggcgc
    25501 gggtatagga gatgttgccc gcactgtcac acatcacaaa aaatccgtcg tcattaatcg
    25561 ccagatccag cgcgttgccg gtggagacca gcgaaccacc gcgcgaaata ctttgcgcgg
    25621 tgcccgccac gctgacgccc atcgcctggc ttccggcata catggctgaa aactgggtgg
    25681 tcatcgactt atagcccacc gttccggcgt tagcgatgtt gttactgatc ccgtcgagct
    25741 gttcgttaac ggcattcagc cccgtcgcgg caatttcata actcattgtg tcttcctgtt
    25801 aattcaaatt ttgacagcgt gcccgttgtt ttgccggatg cggcgtgaac gccttatccg
    25861 gcctacaaaa ccatgcagtt tcatgtaggc ctgataagcg aagcgcatca ggcaatttgg
    25921 caccggacat cacgccacat cactgtcggc atcgggtacg ccaaactggc taatcatggt
    25981 gaacggtact tcgcccacgc ctgcaacgtt aagcaccgga gagctgccgt cgagcgggat
    26041 gcgcacatct tccacttcgc cggagacttc aatcggcacc tcttcttccc cggtgttggt
    26101 gaccaccgaa acgtcgtaat cccccggcgg gataccgtaa tcggcagggt tcaccgagaa
    26161 atcgacctgg cctggcccga cggacgaact gccttccggg atcagcgaca ccgtatagtc
    26221 atcgcctgcg gcatcggtaa agtggagatc ggcggtagta caggcgtcgt ccagcgtagc
    26281 gcgaccgtta atggtctggt cgctcacctg caaggcggtg gtctgcacca taatgtcgtc
    26341 gcccaccagg ttgccgagcg ccattacctg aatgttgctc atcagcaccg cgttggtatt
    26401 cgcctgcacg ctcatctctt ccatcattgc cacctgcgcc atcgacgaaa gctggtcgat
    26461 atattcggtg gcatcggtcg ggtcggtcgg gtcctggttt tggatctcgg cgacaaacag
    26521 cgtcaggaaa gtatcaacgg gcgagctatc gctgctgctt tgcgtgtcgc tggccgccac
    26581 ggtgttgttt tccgccgtgg cggtcggctg aggcgattgc gcattaagcg ccagggtatt
    26641 catcaggctt ctcccagttt cagcaaactt tgctgcatac ttttcacgtt gttgagcaca
    26701 tcgacgttgg tttcaaaatc gcgcgaagcc gacatcatgt ccgccatctg ttccaccacg
    26761 ttgacgtcgg gataccagac atccccgtta gcatccgcca gcggcgaatg cggttcatag
    26821 cgtttcaccg ccccgccggt ttgcagcacg tcctgcacct gcacgctggc gccgtcgatc
    26881 gcgtggcgat gcgttcccgc cagcaagctg ttgtgataca ccgccgcaaa caccgggctg
    26941 cgcgctttat acgcctgcgc ctcgctaccg gcaggcgcct cggcgttcgc caggttactg
    27001 gcgacggtat tcaggcgaat gctttgcgcc gtcatcgccg agccggaaat ctgatagata
    27061 tcagtaaatg acatgcttat ttcccttcaa tggcctctct gatgccgctg atttgcagat
    27121 tcagaaaggt cagactgctt tgataatcca tactattttg tgaaaaccgc gcctgttcgc
    27181 tgttcagttc cacggtgttg ccatcttccg acggctgcat cggcacgcgg tactggacat
    27241 ccaccgccgc gttatttgcc cgttgcatct ccctggcaaa atcaatatct ttagccttga
    27301 aatttggcgt atcgacgttc gccagattcg cagtcagtag ctcggtcctc tcaagacgta
    27361 acttcactgc ctgcggatgt actcccaatg cctgctgaaa actgatcccc atttcctgcg
    27421 tcactcctgc ttcattccgc atacgctagg caattaccgt gccatttttt aagtgactga
    27481 agagaaaggg tttatgagtt ttcaattttg caggcggaag ttttatttcc tcctggcgct
    27541 ggccataccg ggctacgccg ccgtccaccc cgtgcaacac agcgcgcggg aacaagtcaa
    27601 cgcccaggtg ctgaatgccg ccagccagca gattgaatcg ctggcacaac agcggcagtg
    27661 gcatgactat cgctacatct ttaaggtcta tattccgtcg cagattgcga ccgccgcgcc
    27721 ctgtactaaa acaccaggcg tcacgctgac ttcgcctgcg gaaatcgccc tcaaccggat
    27781 gaacttcacc gttagttgcc cgcaaagctg gcagatgaac gtggcggtgc gcccggatgt
    27841 actggtgccg gtggtgatgg caaagtcagt ggttgcccgc gacacgccgc tcacggcaaa
    27901 tgatgtggag ttgaagccct acaacgtcag cgcccagcgc cgggatgtgt tgatggagct
    27961 cgatgacgcg attggtttaa gcagtaagca cgcgctgcaa ccgggcaggc cgctcaccaa
    28021 agaagaactg gtttcgccag tgctggtcga acgcgatcag ccggtgatga ttgtttacca
    28081 gaacgcgggg atcaccgcct cgatgcccgg cgtggcgctg aaaaatggtc gcaaaggcga
    28141 gatggtgaaa gtacgtaacg ccagcagcca gcgggtcatc agcgcaatgg tggcagaaag
    28201 cggcgtggta acgacggtaa gcgcggagta atttgcgcag gcgattaaag ttttgccgcc
    28261 tgttaaccgc tttactggag taacccaccc aaataccgag acgaggttgc gatgaaaatc
    28321 accccgacta tgccaggcaa tcgcccgaca gccacgacgg caggctccag ccagccgcgt
    28381 aaaagcacgg cgaccaccac cacgacgctg agtgccgacg acattactca ggcgggattg
    28441 cagtctgccc agcaaacgct gaacgaccag caagagagcg atatcgacag caacaaagtg
    28501 gcgcaaattc aggcgatgct ggcctccggc agcccgcaag tggacaccga acagttagcc
    28561 agtgacatgc tgagtttttt ccagaactaa gaggccgatg aagtgagtat ccggttacaa
    28621 caggtgaagg cgttgttgca agggatccgc gatgacaaca aaaattatga tggcttgcgg
    28681 gcgctccttg agcagcaacg gctgtgcatg atccgtcgag ccagcgacga gttgctggcg
    28741 gttaatgaga ccatcatgca gcactacgag cggctcaaca gtaccagcct gcaacgccgt
    28801 tcccttttgc agctgcttgg cgtcagcgta aatcgcgccg gtctggagca ggtttttggc
    28861 tggctgcccg cagcgcagaa taatgccgca cgccactggt ggcagcggtt ggagcaaaaa
    28921 gccggaagtt gcaaagccta caacgataaa aacggcgagc tgttgattcg ccagtatgaa
    28981 tttattcagt cttttttagg cagcgaagcg gattttttat atcaggaata acgacaatac
    29041 cttcccccat agcggaaacg gattttccgc atgattctca atcctttgtt tttgattaca
    29101 aaaattcccg gcacgcttat tgcaaatcct gcaaagcatt aaagactgga agccacctat
    29161 gagttttatt gaatgttacg aaccggaata cgtgcgcaat tttatgacgc aacaccccgg
    29221 ttcgccttta tacgtccgca aggtgtggaa aaacgagatt cgcctgagcc tgatgctgac
    29281 agagccagaa agctgtgaag cgatgctcaa cgatgccagg gcctttgatt tacagctcac
    29341 ctatcgctct gtggaaggaa atgccgcaga gctttccgcg cgcgatgcga tactaaacca
    29401 ggtcattttg agcaccctta cgctccccaa tttaccccca gaactatcac tgtacgccgt
    29461 tgggattttg ctctcccgcg ccagcaaaat gccgggaaga gatggcgaca ttcttgcccg
    29521 gctgacaaca ctgccacagg cgctgggcga tcatgcgaaa aaaggcacgc tacaggcaca
    29581 gttcgcccaa ctaccgtccg tgccacagct ggcacgccag ttgatgacgc tgctcggcag
    29641 ttgtgcgttt aactggtcga ttttgccgga aagcccgcgc aaaacgtccc tgccgttgca
    29701 gatgcctttg ctgaccctac atgacgccaa cagcgaagcc ctgttgcagc agcagcttca
    29761 ggggcaatgg caaaccacct ggcaacagca ttttgccacc gcgccctgga tgatgcgtaa
    29821 ctggctgatt taccgcgttt accacgatgt gattgggcag accgacggcg cagattattg
    29881 cccgctggtg tgtgattttt atctcatccg cacgttaatc agtttatgga cgctggatgg
    29941 atcgccactg cggcaggatg atattttcgc gctcttcgcc atgtttgaac gctggcgagt
    30001 gagtgaaaat gctgcggcga ttcgccagca aattcagtcg ttatgtgccg cagagccact
    30061 actttcagcg ttttcgctgc ttacctaacg ccgcgctcga tcggttccct cgcccctccg
    30121 gggagagggt tagggtgagg ggcaaagcgc gcactgctcc cccttaccca tcaaattaac
    30181 ccctaaactc acccaaacac catgccaaca ccaaccatca tcaaaacact cgctacccac
    30241 cgcgaaaaac ccttcgtgag cgtcgtcacg cctacctgga accgtgccac cttcctgccg
    30301 tacctgctct acatgtaccg ctatcaggac tacccggcag accgccgcga actggtcatc
    30361 ctcgacgact ccccgcaaag ccatcaacac atcatcgacc gcctgaccaa tggcacgccg
    30421 gaggccttca acatccgcta catccatcac ccggaaaaac tgccgctggg caaaaaacgc
    30481 aacatgctca atgaactggc agtgggggaa tatatcctct gcatggatga cgacgactac
    30541 tacccggcag ataaaatctc ctggactatc gccatgatgc aaaaacaccg ggcgctcatc
    30601 tccggttccg accagatccc catctggtac agccatatca gccgcatctt tcaaagccgc
    30661 agcttcggcc cgcataacat cctcaacggc accttttgct atcaccgtaa ctacctgaaa
    30721 aagcaccgtt acgatgacga ttgcaattta ggcgaagaga aaagtttcac cgacaatttc
    30781 agcgtaaacc cgctgcaact gcccggcgag cgcacgattc tgtgtatctc gcatagtcac
    30841 aacacctttg ataaagattt tattctcggg gccagcacgc cagtagacgc ggcgcttacg
    30901 gatatcgtca gcgatccgtt gttacgtaat gcttatctga gtctgcataa cgccacccat
    30961 catcaggcga tcaaccatca ggcgatcgat caggtgatca tcgtgaatct ggataaacgc
    31021 ccggatcgct tacagcagat ccgtgaagaa ctggcattgc tgcatattcc gcccgagaag
    31081 atcatccgcc ttgccgccag tgaagatgaa aacggtcaac gtggccgccg ccagtcacac
    31141 ttgcaggcgc tgcgcctggc gcaacaacgc ggctggcaaa actacctgct gctggaagat
    31201 gacgcggtca ttctcaagca ggagaaacat attcaggtac tgaacacgct gctggcgagc
    31261 ctggcgaaaa ttccctggca ggtgatgatc ctcggcggcg aaatttcgca aggaacgatg
    31321 ctaaaaagtt tgccggggct ggtacacgcg cgggactgcc gcaaagtgtg cgcttatctg
    31381 gtaaacagcc gctactaccc gcaactggcg cagcagatga gcaacgatga gcactcgctg
    31441 gaagactgct ggcaaccgct gctacgcgcc gacaaatggc tggcctgtta tccgagtatt
    31501 tgttatcagc gaccgggctt tagtgatatc gagaaaaaaa cgactgacaa cattgctcac
    31561 tatttcaata agttacccgt agccaccaag ccatctacat tacccatcgc cgataccatc
    31621 ggctttttta tggaaacctc attccactac acgctgtacc gcccaatcat caccgctcta
    31681 caggcgcagg ggttgagctg cacactagtg atcaatgacc gcgtgtttaa accgtttctt
    31741 gatgagatgc tggaaacact gaaaaacatc gacgatccgc agcttaaagg tatgcgtctg
    31801 tcagaaatgc aggcacatgg tcagcgagtg aaatgcctgg tcagccccta ccacacgcct
    31861 gcgctcaatg gcctttcacc cgtgaacatc cgcgccatgt acggcttagc taaagagacg
    31921 tggaaccacg ccgactggaa ccgtttttat cagcgcattt tgtgttacag ccactacagc
    31981 cagcaggcgc tggaacattt cggcagcgca aaagtggtcg gcaacccgcg cttcgacgcc
    32041 tggcataacg gcacgtttga tcgcgcgttg ccgggaaata tcgaactgga tgaccgtaaa
    32101 cctacggtgc tctacgcccc aacgtttggc gcactaagct ccctgcccca ctgggcagaa
    32161 aaattgggcc gcttaagcgg tgatgtgaac ctgatttgca aactgcacca cggcacctgc
    32221 tcgcgcccgg aagaagccgc atcgctgacc ctggcgcaca ggcatttaaa acagcgcacc
    32281 aactccgccc cccacacgct ggcgctactg gcaaaggccg attacgtgct gaccgataac
    32341 agcggtttta ttttcgatgc tatccacgtt gataaacggg tgatcctgct cgactttccg
    32401 ggcatgacgg aactgctcga cggtgagaaa agttactcca cgcccgaaag cgccgaccag
    32461 cggattcgcg aaatattgcc ggtggctcat aatgtcgcgg aactgcgtta tttgctgtca
    32521 gaggcgtttg actggggggc ggtgcagacg aagctggcgg aaattcgcca tcattattgc
    32581 gatgcgttta tggatggcaa agcgggagaa cgggcggcga tggtgattgt agaggcgttg
    32641 gggtaagccg cagaagtgcc tgatgcgctt cgcttatcag gcctacgcgc cgctgcaatc
    32701 atttgaattt gcgaggattt gtaggccgga taaggcgttt acgccgcatc cggcacgaac
    32761 agcgcactta tctacccttc gatttcaaac acatctcttt atggctggtt aatcggtaaa
    32821 agttgcacat tttgaaagcg ggaagagctg tctttatgga ttccgaaaaa cgcctttcta
    32881 ccgtcaataa ccattctttt ttacctgtat tggccgcaac atacctcatc ctggaaaggg
    32941 gctaaagcat ttgctggaaa tatctatcac tacaacaggc ctgagcaata tggaacagga
    33001 gggcggtagc gttgctgcaa caatgagctc cgggacttgt tttgcagaat tattttcatg
    33061 tataaggctc atcgctatga ataattttac aaatgataag gtattcaagg cagggccgag
    33121 acgtaagata agagaaacag ccatgaacag aaaccaccta gcgtcagttc ttgcggaatg
    33181 cggactttct taatcttgcc ctgacttatt atactttcaa catgattaat aaattctttt
    33241 agagataaaa taagtgagaa caatttttat aatattcgtt tgttttttca tcgagaatag
    33301 ttatggtagt gaccatctta agattatct
//
LOCUS       DAZUCE010000024        32934 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZUCE010000024 DAZUCE010000000
VERSION     DAZUCE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.024"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            63..680
                     /locus_tag="ACHY03_001051"
     CDS             63..680
                     /locus_tag="ACHY03_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HFS3904311.1"
                     /translation="MSVAALARQRVHRMKRGIPFSISGFYSLGSRASVQQAMSRLVKE
                     GLLVRVERGFYSRPKPLKNIPSVNVVASANAVAAAWAKERGYKITLQGLEAAYRLGLQ
                     TQAPVKRIYWSNGPTREFRVGNERVRIQHKSASRLRWLNRPEGELMRGLLSLDQQHTP
                     LETLKNAFHRLSIPSAKARQIAGKLMNEPALHAWRSQLITLQKAL"
     gene            680..1747
                     /locus_tag="ACHY03_001052"
     CDS             680..1747
                     /locus_tag="ACHY03_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS3904312.1"
                     /translation="MDIFDYYRSASQRRELQEILSYAAQQHPSGLGASFLEKDLWVTE
                     ILRLLFNEELLGGLSVAFKGGTALSKCWNVIERFSEDIDLSVHWADLAGHSEEEEQQA
                     WEQSTLNNSQNRRFREQQQERLTEWTNALAERLNQRFQAYEIPGLEAKMEPDSGGEKV
                     DIYFPRVKEDSMAYQRDHILLEFGGRNRGKPTDLMPVVSYLSGIAGMDTLQLPTATVN
                     AYDTGYILWEKLTALHQFCTQTKAPNPARLARHWYDVDCLLSNHFASPYETLEAMRNV
                     VEMKQRRWSVPGVDFTQVIVGNLQLLPDDAERFAAIAGDHQTAVEGGMFFHQPDRFAD
                     IADRLRHAQDEINRFMRDSEV"
     gene            1931..4258
                     /locus_tag="ACHY03_001053"
     CDS             1931..4258
                     /locus_tag="ACHY03_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HFS3904313.1"
                     /translation="MGFWIFVIMLAVIWLLFSKKKKSPPPRVNNKIITKINHSSRQKS
                     LNKPDNSMTNMHSQASDDDELATFTFVNGQTVEYSTSRQPSRENAARSNTTPARWVKP
                     GESITIQNVVINHGYFYFGGRLKTHSSGEYGYLYNDDSDASLVNDAFPIEPGSRHYYD
                     ESLGYWPSFATLSPRCRGAYLDWLASDRSDASCPVGYVFIYFYGLERRVLADGTQEAI
                     SDDEFKALFEEISRLRTVFQASGSFRHYATQLLEMMIVLRPKLLSIYTENEYFSSRSS
                     LLFRLNLATVVDKGQPICAALALAWIYYFPDYTLRTPARRCHAEFSALFKQRYTQKYG
                     DGIVVKPNKTRLYLSYTPASGTLRELQVKKQMDLPDPSVLKAPVQKLISVAESCINAL
                     DAYSRYLGKKDASPSDVAAIMLLPDEILTEDAERLFAEFKHWADEKIREHSGLATVAD
                     FWARLGMPVPDKINKKEAELMQNFARRAGYGIAPDMRYHLVRPDPEGHLVLFPEGHAE
                     FYVPSAEFTSVSVALRLGAMIAQMDKRVDVAEQAALEKTINHNDALSPTEKRSLHAYL
                     TWRLNTPANQAGLKGKIEQLSDKDKSTIGNVIISVACADGKIDPAEIKQLEKIYASLG
                     LDSSAVTSDIHRLSTAETTPTATLQTPSATSGAFSLDERILARHESDTTDVRQLLNTI
                     FTEDEPADESPAEIPPHAGAGLDEAHHQLYQRLQEKERWARNEVAELCQQFNLMLSGA
                     IEAINDWSFEQVDAPVLDDDDDIYVDLEIAQELKG"
     gene            4262..5578
                     /locus_tag="ACHY03_001054"
     CDS             4262..5578
                     /locus_tag="ACHY03_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS3904314.1"
                     /translation="MSGIRIRLKERDAIIQSLKSGVTPKIGIQHIQVGRVNEIKALYQ
                     DIERIADGGAGFRLIIGEYGSGKTFFLSVVRSIALEKKLVTISADLSPDRRIHATGGQ
                     ARNLYSELMKNLSTRNKPDGNALLSVVERFITEARKEAESTNVSVPTIIHQKLAALSD
                     MVGGYDFAKVIECYWQGHEQDNETLKSNAIRWLRGEYTTKTDARNDLGVRTIISDASF
                     YDSLKLMSLFVRQAGYAGLLVNLDEMVNLYKLSNTQARVANYEQILRILNDCLQGTAE
                     YIGFLLGGTPEFLFDPRKGLYSYKALQSRLAENSFAQRAGVIDYSSPSLHLASLTPEE
                     LYILLKNLRHVYSGGDADKYLVPDDALTAFLRHCSNTIGDAYFRTPRNTIKAFLDMLA
                     VLEQNPSIQWSQLIAGVAIAEEKPSDMDEITSAEDADEDGLADFRL"
     gene            5575..7773
                     /locus_tag="ACHY03_001055"
     CDS             5575..7773
                     /locus_tag="ACHY03_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS3904315.1"
                     /translation="MMNEYQRLDPRIQKWIYRQGWADLRELQKKSVSPILAGDRDVLI
                     SAATAAGKTEAFFLPACSAIADIQGGFGILYISPLKALINDQYRRLENLGDALEMPVT
                     PWHGDVAQSKKLKAKKNPAGILLITPESLEAMLIRNAGWLKQAFAPLAYIAIDEFHAF
                     IGSERGMQLLSLLNRVDHLLGRINNPVPRVALSATLGELEQVPLSLRPNQRLPCDIIT
                     DSQTHATLKVQVKGYLEPLTTSGQQSPPSAETQICHDIFRLCRGDSHLVFANSRKRTE
                     SIAATLSDLSEASIVPNEFFPHHGSLSRDLRETLEQRLQQGNLPTTAICTMTLELGID
                     IGKVSSVVQVTAPHSVASLRQRMGRSGRRDSPAVLRMLIAEHELTPTSGIVDQLRLQL
                     VQSLAMIRLLIGNKWFEPADTRQMHYSTLFHQILAIVAQWGGVRADQIWSQLCLQGPF
                     QKVRIYDFKTLLKHMGEHQFLTQLSSGELVLGVEGERQVNQYTFYAVFSTPEEFRIVA
                     GSKTLGSIPVDSPLMPDQHIIFGGRRWKVTDIDSDKKVIYVEATKGGQPPLFGGQGMS
                     IHDVVRQEMLTIYREGDYRITVGNRKADFADTTAKNLFDEGLHCFRNNNLASECFIQQ
                     RQHVYILPWLGDQTVNTLSALLIQRGFKAGSFAGVVEVEKTTVSEVKQALFSALQEGL
                     PYESRLAESIVEKCLEKYDEYLPETLLTQEYGLRAFNIERVTEWLQGHLY"
     gene            7785..12500
                     /locus_tag="ACHY03_001056"
     CDS             7785..12500
                     /locus_tag="ACHY03_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904316.1"
                     /translation="MRKSWTIEEDCKLLTLVRQLFSALVSHNRLNATMPFSQQLHDAF
                     DSPDRDAAALLYRLEQAKILGFASRPGGDPTKQLFRCLISNDLALYDYSLTFPTLRKA
                     LHPDTVAAALNHFTISNPHEPLSNTINEIATALHLAPMQVEKILIDSGQITINSYRKC
                     ERVGEKNINNNLQDLISRQIPDITLIKEINACRAQVSQLYHVHERDGAEVIFSSDGTG
                     FGKSYGVIQGYVEYLERFAKTQKSDDLFPEGGFTNLLFMSPQKSQIDLDSSQKEKILA
                     ASGEFICVLSRKDVADLDFMDWASGLKNRDRYIQWYEGAKGSKYIGGAMRSLNYHVLQ
                     INRCEEQLKKLTTYGSQDTNYEREILEEQLKNCRHSIRNTIESACKLLFGPDSEKASI
                     KEYIRRGLQARQERMQNAETARKPGKLEPKISVHEVYFELIKQVLPFEVCQYRPSVLL
                     MTTNKFDTSTYRLAPRQRGEGVRFESVGFDLLIGGKLTPKDPQISTVAAAGHTGQVTY
                     LRDEHFRRNPDCPFRQKNIRFTVIIDELHEAYTRLEETCHVKLITQENNLAHVISVAG
                     RIHNAVLSLERRNKPKEAQTTFEQEMVKFITTLRNLLAEKCELSPGTRLGSILEMFRD
                     QLGAFEVNGDAAERIISITRNVFSFNPKMYVNEEGLKRIRMRNSEGDITRTELYYEVE
                     NDANDTNPTLHDLFQLVSVILAACSEITNRHFKRWVKNGGQDNSSSQNTPLGQFVDAA
                     NNVAGVVRHIFDRTTDKNLLIDHFYTYLQPKTVFTMTPIAELNYVNRGAERTIILAFE
                     MDLVQELPEAMLLRLLTGTHNKVIGLSATSGFSHTKNGNFNRHFLAHYSRDLGYRVVE
                     REKADIDTLKALRGLRASIRNVDFRVFDDKQLKLTDIYQNCEIYRRTYDNFFDALKKP
                     LEYDLKNTYKRRQCQRELEALLLAAWEGKNSLILSLSGTFKRAFISAWRTHQTTWRQQ
                     YGMHSRCDEKTDNGKKHDQILTFTPFKGRHTVHLVFFDSPLANVEDIRQETYLQNSNT
                     VLVFMSSYKSAGTGLNYFVKYHDGDINDINAPRLDVDFERLVLINSSFYSEVKDNSGN
                     LNTLPNYVTVLKHYADDDITVHKLADFNVNFAHGENYRLLMAEHDMSLFKVVVQAVGR
                     VERRDTLLKTEIFLPRDVFRNVAFQFAALSEDSGNEVVSESMSLLNHRLMEECEKLSQ
                     GQSFNNAEQRLTFEQAIVANGRRIDEIHKRVLKTDWINKVRAGNLDYLEICNLFRDPD
                     SFTDPQRWLAKLQANPLYTANRQMQSVHDALFIDRQQGNQTILLCHKRGPDGLAHRDY
                     SALSDFAGGAREYRPELTLFPQYRNDVDFTPGNLVGELIRECDNIQEKAFKKWVPNPK
                     LVPLLKGNVGEYLFDKVLKSYGVTPLSDQQVFERLEPLVYEFFDRFIEVGDDLLCIDV
                     KRWATQLDDLTRAEETLEKSDNKIRQIRNIASQKADTEGQKQLQTALAGRYERIRFIY
                     LNVAYSQNPNNLMWQDNVDHTIHYLNLLQTDYQYYQPKNRESGRAQENSKLRMTLDIN
                     PMLLTLLGVEKLPTKGKVS"
     gene            12497..14728
                     /locus_tag="ACHY03_001057"
     CDS             12497..14728
                     /locus_tag="ACHY03_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904317.1"
                     /translation="MIPNLNELTDTPIARTNLIKLEEDQLTTIQRLLAPVSNIYTIDF
                     MVQHFTKERKEKSADYYARIHQEVKTCVRQKLGLEAGQEVKYELHCLPNYHHVFFFLA
                     PAAAPNSLAHRTLAERIETLCQRLTAENYDLSRLIQGLFSLHLKMVMLEQASERFSVP
                     PTYFNSTFYLNARLSQPVTQKSGTGVMEAFELDIYASEYNELAFTLHKRKFLVEPEDE
                     LHLSLDDTCVWFNIDNRRLKARRKLDARDSKLDFFRERSGYGECQAYTYNVVMNAACE
                     RLSELEIPHQPIAFQATHEVNQFATDLDQQLTNTLLVVNNGVEFSATQEAYFFDTLAI
                     QFPGYQLWPLASLKHSQQTGFSELPASTSILVLNAVDEERSNSIRQQDNESVEYNDFY
                     AAFADARKQPELNWDTYTQLKLDRLQGWLNQQPLPVVLQGMNIDHKLLDAIDFINEQL
                     TSNPTQYEIDLTKPHSRLKSAVTLLNSKVRRTKTELWFKESLLNQHHIPLPDLADGHY
                     TAYAVRKTKSYLPLLGYVELKIEHGQLRVVDTGIAEGKLDYLSVDPPSLGRLKKLFDK
                     SFYLYDHTADVLLTTYNSSRVPRLIGPAQFNIVDSYAYQEQEKTLAERKGDKFNGYAI
                     TRSAKPDQNVLPYLISPGRSKYDSLTKAQKMKHHHIYLQPHENGVFVLVSDAQPTNPT
                     IARPNLVENLLIWDAQGKAVDVFSHPLTGVYLNSFTLDMLRSGESSKCSIFAKLARLM
                     VEN"
     gene            14857..15956
                     /locus_tag="ACHY03_001058"
     CDS             join(14857..15112,15112..15956)
                     /locus_tag="ACHY03_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611505.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFS3904318.1"
                     /translation="MKKTRYTEEQIAFALKQAETGTRVGEVCRKMGISEATFYNWKKK
                     FAGLGVTELRRLRQLEDENQRLKKLVADLSLDKEMLQEVLKPKVLRPAQKRQAVHFLR
                     EAYRISVRRGCGLLIQSRTVYHWQSRRDDRAITLRIREIAETRIRYGCPRIHIQLRQE
                     GWPVNHKKTHRIYCLEGLNLRRKRPCRHVSAAHRQQRPILTGVDQCWSMDFVSDNLFN
                     GRRFRALTVVDNFSRECLAIHAGKSLKGEDVVGVMEGLRVPGKRLPVRIQTDNGSEFI
                     SKSLDKWAYEHGVTMDFSRPGKPTDNPFIESFNGSLRDECLNIHWFLSLEDAQDKLDS
                     WRREYNHERTHSSLNDMTPAEFIRSLRKDEDL"
     gene            complement(16312..16848)
                     /gene="yghD"
                     /locus_tag="ACHY03_001059"
     CDS             complement(16312..16848)
                     /gene="yghD"
                     /locus_tag="ACHY03_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFS3904319.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAA
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIRRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(16850..18028)
                     /gene="gspL"
                     /locus_tag="ACHY03_001060"
     CDS             complement(16850..18028)
                     /gene="gspL"
                     /locus_tag="ACHY03_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFS3904320.1"
                     /translation="MSSILEIFFPLCAANPIRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSMPCGYMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRAQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQTDDTRLLSELSAIASILKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRTALAADFVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(18025..19002)
                     /gene="gspK"
                     /locus_tag="ACHY03_001061"
     CDS             complement(18025..19002)
                     /gene="gspK"
                     /locus_tag="ACHY03_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFS3904321.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNVLAQPTTASRPLALQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVIWHQTGESE"
     gene            complement(18999..19604)
                     /gene="gspJ"
                     /locus_tag="ACHY03_001062"
     CDS             complement(18999..19604)
                     /gene="gspJ"
                     /locus_tag="ACHY03_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFS3904322.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQENWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(19601..19972)
                     /gene="gspI"
                     /locus_tag="ACHY03_001063"
     CDS             complement(19601..19972)
                     /gene="gspI"
                     /locus_tag="ACHY03_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFS3904323.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGNRWYWRTTPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(19969..20532)
                     /gene="gspH"
                     /locus_tag="ACHY03_001064"
     CDS             complement(19969..20532)
                     /gene="gspH"
                     /locus_tag="ACHY03_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFS3904324.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLSQCDERMP"
     gene            complement(20536..20991)
                     /gene="gspG"
                     /locus_tag="ACHY03_001065"
     CDS             complement(20536..20991)
                     /gene="gspG"
                     /locus_tag="ACHY03_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFS3904325.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(21008..22231)
                     /gene="gspF"
                     /locus_tag="ACHY03_001066"
     CDS             complement(21008..22231)
                     /gene="gspF"
                     /locus_tag="ACHY03_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFS3904326.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIET
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSVHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDTLQTSGVYWLAGLLGLLVLGQRLLKNPAMHLRWDKTLLRLPVIGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(22231..23724)
                     /gene="gspE"
                     /locus_tag="ACHY03_001067"
     CDS             complement(22231..23724)
                     /gene="gspE"
                     /locus_tag="ACHY03_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFS3904327.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDVRTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQRSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(23724..25784)
                     /gene="gspD"
                     /locus_tag="ACHY03_001068"
     CDS             complement(23724..25784)
                     /gene="gspD"
                     /locus_tag="ACHY03_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFS3904328.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(25814..26773)
                     /gene="gspC"
                     /locus_tag="ACHY03_001069"
     CDS             complement(25814..26773)
                     /gene="gspC"
                     /locus_tag="ACHY03_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFS3904329.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIARGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTVVSPSVNKPPRTDAKTFDKNDVQLISQQNWF
                     GKYQPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLVEEERSTVAVTNKKAISDEVKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(26791..27201)
                     /gene="gspS2"
                     /locus_tag="ACHY03_001070"
     CDS             complement(26791..27201)
                     /gene="gspS2"
                     /locus_tag="ACHY03_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFS3904330.1"
                     /translation="MSIKQMPGRVLISLLLSATGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCTDPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(27267..28076)
                     /gene="pppA"
                     /locus_tag="ACHY03_001071"
     CDS             complement(27267..28076)
                     /gene="pppA"
                     /locus_tag="ACHY03_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFS3904331.1"
                     /translation="MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(28403..>32934)
                     /gene="sslE"
                     /locus_tag="ACHY03_001072"
     CDS             complement(28403..>32934)
                     /gene="sslE"
                     /locus_tag="ACHY03_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFS3904332.1"
                     /translation="LLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTP
                     NPEPTPEPTPDPEPTPEPTPDPEPEPEPEPVPTKTGYLTLGGSQRVTGATCNGESSDG
                     FTFTPGDKVTCVAGNSTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKSNA
                     LSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAAT
                     DKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSEGRLVDSQGYGV
                     AGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRG
                     ANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLGEGEQVVN
                     LPNEFIEQFKTGQANEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDT
                     NYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAW
                     DKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGDGKLMVIGNPHYNSILRCPN
                     GYSWNGGVNKDGQCTLSGDSDDMKHFMQNVLRYLSDDKWTPDAKASMTVGTNLDTVYF
                     KRHGQVTGNSAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGNDPYA
                     IPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLS
                     MALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTGEVKWKYQVE
                     NKPDDKPKLEVASWQEEVEGKQVTRYAFIDEAEFKTKESLEAAKAKIFEKFPGLQECK
                     DSTYHYEINCLEYRPGTKVPVTGGMYVPQYTQLNLSADTAKAMVQAADLGTNIQRLYQ
                     HELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGFKTFTEFLNC
                     YTNDAYTDGTQCSADLKKSLVDNNMIYGESSGKAGMMNPSYPLNYMEKPLTRLMLGRS
                     WWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTIK
                     SNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYIK
                     GNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTTPKKNLNASN
                     YTGGLEQFANDLDTFASSMNDFYGRDETSGKHRMFTYKALTGHKHRFTNDVQISIGDA
                     HSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYM
                     QDRYLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQLKEWAEKNFDIKKW
                     YPDGTPLPEFYSEREGMKGWNLFQLMHRKARGDDVGNDKFGNRNYCAESNGNAADKLM
                     LCASWVAQTDLSEFFKKWNPGANAYQLPGVSEMNFEGGVSQSAYETLAALGLPKPQQG
                     PETINQVTEHKMSAE"
BASE COUNT         8019 a         8833 c         8217 g         7865 t
ORIGIN      
        1 cttgctacac aagggatata atgaaaatac atatcaatga aaacgtcagt acaggagaat
       61 caatgtctgt tgccgctctg gcccgccagc gggttcaccg catgaagcgt ggcatcccct
      121 tttccatcag cgggttttac tcgctgggaa gccgtgcgtc ggtacaacag gcaatgagcc
      181 gtctggtcaa agaaggcctg ctggtccggg ttgaacgcgg tttctactcc cggccaaagc
      241 cgttgaaaaa catcccctct gttaacgttg ttgccagcgc caatgccgtt gccgccgcat
      301 gggcaaaaga gcgtggttac aaaatcaccc ttcaggggct ggaagccgct taccggctgg
      361 ggctgcaaac gcaggcgcca gttaaacgca tctactggtc aaacggccct acgcgagaat
      421 tccgcgtcgg caatgagcgg gtacggatcc agcataaatc agcaagtcgg ttacgttggc
      481 tgaaccggcc tgaaggagag ctgatgcgcg gtttgctgag tctggatcag caacataccc
      541 cattggaaac gctgaagaat gcttttcatc ggctgagcat cccgtccgct aaagcacggc
      601 agattgccgg aaagttgatg aatgagcctg cgcttcacgc gtggcgctcg cagctgataa
      661 cgttgcaaaa ggcgctctga tggacatttt tgattactac cggagcgcat cacaacgtcg
      721 cgagcttcag gaaatcctct cttacgcggc gcagcagcat ccctccgggc tgggagccag
      781 ctttctggaa aaggatctct gggtaacgga gatccttcgg ttacttttta atgaggagct
      841 gctgggaggt ttatctgtcg cgtttaaagg tggaacggcg ttaagcaaat gctggaacgt
      901 gattgagcgt ttttctgaag atatcgatct ttctgttcac tgggccgatc tggcaggcca
      961 ctcagaagag gaagaacagc aggcatggga gcaaagcacg ctaaataaca gccagaatcg
     1021 ccggttcaga gagcagcagc aagagcgctt aacagaatgg acaaacgcgc tggcagaacg
     1081 gttaaaccaa cgctttcagg cttatgaaat tccgggcctt gaagcaaaaa tggaacccga
     1141 cagtggtggc gaaaaagtcg atatctattt cccacgggtg aaagaagaca gtatggccta
     1201 tcagcgcgat catattctgc tggaatttgg cggacgaaat cgtggcaagc caaccgatct
     1261 gatgcctgtt gtaagctatc tgtccgggat cgcaggcatg gatacactcc agttacccac
     1321 cgcgaccgta aacgcttatg acaccggtta tattctttgg gaaaagctga cggcgctgca
     1381 ccagttctgc acgcaaacca aagcgccaaa tccggcccga ctggcacgcc attggtatga
     1441 tgtggattgt ctgctgagta atcattttgc cagcccctat gaaacgctgg aagccatgcg
     1501 caatgtggtc gagatgaagc aacgtcgctg gtctgtgccg ggtgtggatt ttactcaggt
     1561 gattgtcgga aacctgcaac tgttgcctga cgatgctgaa cggttcgcag cgatcgccgg
     1621 ggatcatcaa acggcggtgg aagggggaat gttctttcat cagcctgaca gatttgctga
     1681 cattgccgat cgtcttcgtc atgcgcaaga tgaaatcaat cgttttatgc gtgattcaga
     1741 ggtataacct ggtcctgcca attgctcccc cagccatatg acataatcct tttgaataat
     1801 agggttttta tgcttgtact ctagcccatt cgcggtatca ttttacgatc tctcttccag
     1861 ttttatgctt accgcctttg cctatcgtag aacaatgccg ggaagcgtta tcagcgatta
     1921 agggcaagga atgggcttct ggatatttgt tattatgctg gcggttatct ggcttctgtt
     1981 ttccaaaaag aaaaaatcgc cgccccccag agtaaacaac aaaatcatca ccaaaataaa
     2041 tcattcatct cgacagaaat ctctcaataa gccagataac agcatgacaa atatgcattc
     2101 tcaggcctcc gatgatgacg aactggcaac ctttactttt gtgaacgggc agacggttga
     2161 atacagcacc agccgccagc cgtcacgaga aaacgccgcc cgtagcaata ccactccagc
     2221 gcgatgggtc aaaccgggag aaagcatcac cattcaaaat gtcgtcatta atcacggtta
     2281 tttttatttc ggcgggcggt taaaaacaca ttcatcagga gaatatggat atctttataa
     2341 cgatgactcc gacgcttcgc tggttaatga cgcttttccc atcgagcctg gttcacggca
     2401 ttattatgat gagtcactgg gatactggcc cagctttgcc acactctccc ctcgctgccg
     2461 tggcgcctat cttgactggc tggcaagcga tcgcagcgat gcgagctgcc ccgttggcta
     2521 tgtttttatc tatttttacg gtctagaacg ccgcgtactg gccgatggca cacaagaagc
     2581 catttctgac gatgaattca aagcattatt cgaagagata tcgcgcctga gaaccgtatt
     2641 tcaggcaagc ggttccttcc ggcattatgc aacgcagttg ctggaaatga tgatcgttct
     2701 ccgaccgaag ttgctttcta tatataccga aaacgaatat ttctcatcga ggagttcatt
     2761 actgttcaga ttaaatctag cgactgtggt cgataaagga caacctattt gtgccgctct
     2821 ggcactggca tggatatact attttcctga ttacaccctg cgcacgcctg cccgtcgatg
     2881 tcatgctgaa ttttccgcat tattcaaaca gcgttatact caaaaatacg gtgacggtat
     2941 tgtcgtcaaa cccaataaaa cacggttgta tttaagctat acccccgcca gtggtacgct
     3001 tcgggaactt caggtaaaaa aacagatgga tcttcccgat cccagcgttt taaaagcccc
     3061 agttcagaaa ttaatttctg ttgcagaatc ctgtatcaac gcgctggatg cctacagtcg
     3121 ctatctcggt aaaaaagatg cctcaccaag tgatgtcgcc gccatcatgc tgcttcccga
     3181 tgaaatactg accgaagatg cagaacgtct atttgctgaa tttaaacact gggcagatga
     3241 gaaaatccgt gaacattcag gactggcgac agtggctgat ttctgggcca gactgggtat
     3301 gcctgtaccg gataagatta ataagaaaga agccgagctg atgcaaaatt tcgcccggcg
     3361 agcaggctac ggcattgcgc cggatatgcg ctatcacctt gtcagaccgg atccagaagg
     3421 tcatcttgtt ttatttcctg aagggcatgc ggaattctac gtaccgtcgg cggaatttac
     3481 gtcagtctct gtggcgcttc ggttgggtgc catgattgca caaatggaca agcgcgtgga
     3541 tgttgctgaa caggccgcgc tggagaaaac gattaatcat aacgatgcgc tgtcgccaac
     3601 agaaaaacgt tcgctgcacg cctacctcac ctggcggctc aatacgcctg caaatcaggc
     3661 tggtctgaaa ggtaaaattg agcaactcag cgataaagat aaatccacta ttggcaacgt
     3721 gattatcagc gtcgcctgcg cagatggaaa aatcgatccg gctgaaatca aacaactgga
     3781 aaaaatctac gccagcctcg gtctggacag cagtgccgtt accagcgata tccaccgact
     3841 gtcaaccgca gaaacaactc cgacagctac gttacaaacc ccatcagcga cgagcggcgc
     3901 gttttctctt gatgaacgga tccttgcccg tcatgaatcc gacacaacgg acgtacgcca
     3961 gttactgaac accatcttca ccgaagatga acccgcagac gaatccccag cggagatccc
     4021 gccacacgct ggcgcaggtc ttgatgaagc acatcatcaa ctttaccaac gtttgcagga
     4081 aaaagaacgc tgggcgcgaa acgaagtcgc tgagctatgc cagcagttta atttgatgct
     4141 aagcggcgcg attgaagcaa ttaatgactg gtctttcgaa caggttgacg ccccggtgct
     4201 tgatgatgac gatgatattt acgttgacct ggaaattgca caagaactca aaggataatt
     4261 tatgtctggc attcgtattc gtctcaaaga aagagacgct attattcagt cactgaagtc
     4321 aggtgttacg cctaaaattg gtattcagca cattcaggtt ggccgggtca acgaaataaa
     4381 agcgctgtat caggatattg agcgtatcgc tgatggcggc gcaggattcc ggctgattat
     4441 tggggaatat ggctcaggta agacattctt tttaagcgtt gtgcgctcaa ttgcgctaga
     4501 aaaaaagctg gtgacaatca gcgccgattt atccccggac aggcgcatcc acgcgacggg
     4561 tgggcaggcg cgtaacctct actccgagct aatgaaaaat ctatccaccc gaaataagcc
     4621 ggatggaaac gcattattaa gcgtggttga gcgctttatc acggaagcca gaaaagaagc
     4681 agaaagtaca aatgtgtcag ttccgacgat tattcaccaa aagctcgccg ccctgtctga
     4741 tatggttggc ggttacgatt tcgccaaagt cattgaatgt tactggcagg gccacgagca
     4801 ggataatgag acattgaaat caaatgccat ccgctggcta agaggtgaat acaccacgaa
     4861 aaccgacgcc cgtaacgatc tgggtgtgcg caccattatt tctgatgcct ctttctacga
     4921 ttcgctaaag ctgatgagcc tgtttgtccg tcaggccgga tacgcgggtc tgctggtgaa
     4981 tctggatgag atggtcaatc tgtataagct cagtaacact caggcccgcg ttgccaacta
     5041 tgaacagata ctgcgtattc tgaatgactg cctgcaaggg acggctgaat atatcggttt
     5101 tttacttggc ggtacgccag aattcctgtt cgatccgcgc aaggggttgt acagctacaa
     5161 agcgctccag tcccgactgg cggaaaatag cttcgctcag cgggctggtg tcattgatta
     5221 ttcgtcccct tccctgcact tagccagcct gacgccggaa gaactctata ttctgttgaa
     5281 aaaccttcgt cacgtttatt ccggcggcga tgcggataag tatctggttc ctgatgatgc
     5341 tctgacggca tttttacgcc actgtagcaa cactattggc gatgcctatt tccgtacgcc
     5401 acgaaacacg attaaagcct tcctggatat gctggccgtg ctggaacaaa acccatccat
     5461 tcagtggtca cagttaatcg ccggtgtcgc gatcgcggaa gaaaaaccca gtgatatgga
     5521 tgaaataaca tcggcagaag atgccgatga ggacggtctg gccgacttca gattatgatg
     5581 aacgaatacc agcggctgga tccacggata cagaagtgga tataccggca gggatgggcc
     5641 gatctcaggg aactgcaaaa aaaatccgtt tcaccgatat tagcgggcga tcgggatgtt
     5701 ctgatcagcg ccgcgactgc cgcaggtaaa acagaagcgt ttttcctgcc cgcctgttct
     5761 gccattgcgg atattcaggg cggctttggc attttataca tcagcccgct taaggccctg
     5821 attaacgatc agtatcgaag gctggaaaac ctcggtgatg cgttggagat gccggtcacg
     5881 ccctggcatg gtgatgttgc gcagagcaaa aagctgaaag caaagaagaa tcctgccggt
     5941 attttgctta tcaccccgga atcgctggaa gcgatgctga tccgcaatgc gggatggtta
     6001 aagcaggctt tcgcgccact ggcatatatc gccattgatg aattccatgc tttcatcggt
     6061 tctgagcggg gtatgcagct tctctctctg ttaaatcgag tcgatcacct gctgggaaga
     6121 atcaacaatc cagtcccccg agtcgcactc agcgcaacgc tgggggaact ggaacaggtg
     6181 ccgttatctc tgcggccaaa tcaacgtctg ccctgtgaca ttattaccga cagtcagact
     6241 cacgccacgc taaaagtaca ggtgaaaggt tatctggaac cgctgaccac ctcgggccag
     6301 caatctccac cgtcggcaga gacgcaaatc tgccatgata tctttcgcct ctgtcgtggt
     6361 gattcccatc tggtgttcgc taatagtcgc aaacggaccg aaagcattgc cgccacgctt
     6421 agcgatctca gtgaagcgag catcgttccc aatgagttct ttccccatca cggatctctg
     6481 tccagagatc tgcgtgaaac gctggaacag aggcttcaac aaggcaactt acccaccacc
     6541 gccatctgta cgatgacgtt agagcttggc atcgacatcg gtaaagtcag ctccgttgtg
     6601 caagttaccg ccccccattc cgtagccagc ctgcgtcagc gaatgggacg ctccggtcgg
     6661 cgcgactcgc ctgccgtatt gagaatgctg attgccgaac atgaactgac gccaacatca
     6721 ggcattgtcg accagctcag gcttcagctt gttcagtcgc tggccatgat ccgcttactt
     6781 atcggcaaca aatggtttga gccagctgat acccggcaga tgcactattc caccctgttc
     6841 catcagatcc tggcgatcgt ggcgcagtgg ggaggcgtgc gtgcggatca gatctggtca
     6901 cagctatgcc tgcaagggcc atttcagaaa gtccggatct atgacttcaa aacgttattg
     6961 aaacatatgg gggagcacca gtttctgacc cagctctcaa gcggcgaact ggttctgggc
     7021 gtcgagggcg aacgtcaggt aaatcaatac accttctacg ccgtgttcag cacgccggaa
     7081 gagtttcgca ttgtggcggg gagcaaaaca ctgggctcca ttcccgttga ttccccactg
     7141 atgcctgatc aacacattat tttcggcggt cgacgctgga aggtaaccga tatcgatagt
     7201 gataaaaaag ttatttatgt cgaggcgaca aagggtgggc agccgccgtt atttggcgga
     7261 caagggatgt ccattcatga tgtcgtccgc caagaaatgc tcactattta tcgggaaggc
     7321 gactaccgca tcaccgttgg caatcgcaag gccgattttg ccgataccac ggccaaaaac
     7381 ctgtttgatg aagggctgca ctgttttcgc aacaataatc tggcttcgga atgttttatt
     7441 cagcagagac agcatgtcta cattcttccc tggctaggcg atcaaaccgt aaacacgttg
     7501 tcggcattac ttatccaacg cggtttcaag gcgggctcat ttgctggtgt ggttgaagta
     7561 gaaaaaacta cggtctcgga ggttaaacaa gcgttattca gcgcacttca ggaagggcta
     7621 ccttacgaat cccgtcttgc cgaaagcatc gttgaaaagt gcctcgaaaa atatgatgag
     7681 tatttacccg agacgttgct gacgcaggaa tatggattac gtgcttttaa tattgaacgc
     7741 gtgacggagt ggttgcaggg gcatttatat taaggggaag aagaatgaga aaaagctgga
     7801 ctatagagga agattgtaag ctgctaacct tggtgcgtca gctcttttcc gcgctggtca
     7861 gccataaccg gctgaatgcc acaatgccat ttagccagca gctccacgat gcatttgact
     7921 cacctgaccg cgatgccgca gcattgcttt atcgcctcga acaggcaaaa atcttgggat
     7981 ttgccagccg tcctggtggc gatcccacta aacaactgtt tcgctgcctg ataagcaatg
     8041 atttggcgct atacgattac agcctcacct ttcccaccct cagaaaagca ttgcatccag
     8101 ataccgttgc ggcagcacta aaccacttca cgattagcaa tccacacgaa ccactgtcca
     8161 atactatcaa tgaaatcgcg acagccttgc atcttgcccc catgcaggtg gaaaagattc
     8221 tgatcgacag cggccaaata accatcaata gttaccgcaa gtgtgagcgt gttggagaga
     8281 aaaatatcaa taataatctg caagatctca tctctaggca aattcctgac ataacgctga
     8341 ttaaagagat taacgcctgt cgcgcccaag tctctcaact ttaccacgtg catgaacgtg
     8401 atggcgctga ggtcatcttc agttccgacg gcacggggtt cggcaaaagc tatggcgtga
     8461 tccaagggta tgtcgaatat ctggagcgct tcgccaaaac ccaaaagtca gacgatctgt
     8521 ttcctgaagg tggctttacc aacctgctat tcatgtcacc gcaaaaatca caaatcgacc
     8581 tggacagcag tcagaaagag aaaattctgg ccgctagcgg cgagttcatt tgcgttctct
     8641 cccgtaagga tgttgccgac ctcgacttta tggactgggc ctctggtctg aaaaaccgcg
     8701 accgctatat tcagtggtac gaaggggcga aaggcagcaa atatatcggc ggcgctatgc
     8761 gttcgctcaa ttatcatgtc ttacaaatta atcgctgtga agagcagtta aaaaagctga
     8821 caacatacgg ttctcaggat accaactacg aaagagaaat tctcgaagaa cagctaaaaa
     8881 actgccgtca cagtatccgc aatacgattg agtcagcctg taaattacta tttggaccag
     8941 atagtgaaaa agcttccatt aaagagtaca ttcgtcgcgg gctccaggcg cggcaagagc
     9001 gaatgcaaaa cgcggagaca gcacgaaaac caggaaagct tgaacctaag ataagcgtac
     9061 acgaagtcta tttcgagctt atcaaacagg tattgccttt cgaagtttgc cagtaccgcc
     9121 cgtcagtgct attaatgacc acgaataagt tcgacacatc aacttaccga ctggcgcctc
     9181 gtcagcgagg cgaaggtgtg cgttttgagt ccgtaggttt cgacttgctg attggcggta
     9241 agctgactcc caaagatcca cagattagca ccgttgcggc agccggtcat accgggcagg
     9301 ttacctatct tcgcgacgaa cacttcagac gcaatccaga ttgtcctttt cgccagaaaa
     9361 atattcgttt tacggtgatc attgatgaac tacatgaagc ctacactcgc cttgaagaaa
     9421 catgccatgt aaagctaatc acacaggaaa ataacctggc gcacgttatt tccgtcgcag
     9481 gacgtattca caacgcggta ctcagcttag aacgccgaaa caagcccaaa gaagcgcaaa
     9541 cgacctttga gcaagagatg gtcaaattca tcactactct gcgcaattta ctggcggaaa
     9601 agtgcgaact atcccccggt acaaggctgg gatcgatcct ggagatgttt cgtgaccagt
     9661 taggggcatt tgaagtcaac ggcgacgccg ccgaacgcat catctcaatc acccgcaacg
     9721 tattcagctt taaccccaaa atgtacgtca atgaagaagg gctgaaacgc attcgcatgc
     9781 gcaacagcga aggcgacata acgcgcaccg aactgtatta cgaagtcgaa aatgatgcca
     9841 atgacaccaa ccccactctg cacgatctgt tccagttggt ctccgtcatc ctcgccgcct
     9901 gttctgaaat caccaaccgg cactttaagc gctgggtaaa gaatggtggc caggacaact
     9961 ccagcagcca gaatacgcct ttgggccagt ttgttgacgc agccaataac gtagccggcg
    10021 tggtgcgaca tatcttcgat cgcaccaccg ataaaaactt gttgattgat catttctaca
    10081 cttacctgca acccaaaacc gtattcacga tgacgccgat agctgaactc aattacgtga
    10141 acaggggagc cgagcgcaca attattctgg cgttcgagat ggatctggta caagagttgc
    10201 ctgaagccat gctgctgcgt ttattaaccg gcacgcacaa taaagtaatt gggcttagcg
    10261 ccaccagcgg ttttagccac accaaaaacg gtaacttcaa tcgtcacttc ctggcgcact
    10321 atagccgcga ccttggctac cgggtcgttg aacgcgaaaa ggcagatatc gatacgctta
    10381 aggcattacg cgggttgagg gccagtatcc gcaacgtaga cttcagggtg ttcgatgata
    10441 agcagttaaa attgaccgat atctaccaaa attgtgaaat ctatcgcagg acgtatgaca
    10501 actttttcga cgcgctgaag aaaccgctgg aatacgacct gaaaaatacc tataaacggc
    10561 gtcagtgcca gcgggaactg gaagcgttac tgcttgccgc ctgggagggt aaaaacagcc
    10621 tgattctgtc actttcaggg acgtttaagc gggcctttat cagcgcctgg cgcacgcacc
    10681 agacaacctg gcgtcagcag tacggtatgc actcccggtg cgatgaaaaa acggataacg
    10741 gtaagaaaca tgaccagatc ctgaccttta ccccattcaa agggcgtcac accgtccatt
    10801 tggtcttttt cgattcacca ctggctaatg tcgaagatat caggcaagaa acctatctcc
    10861 agaacagcaa taccgtactg gtatttatga gcagttataa aagtgcgggt accggcctca
    10921 actactttgt taaataccat gacggcgata ttaatgatat caatgcacca cgtctggatg
    10981 tcgattttga gcgcttagtg ctcatcaact cctcgtttta cagcgaagta aaggacaaca
    11041 gcggcaacct caatacatta cctaactacg ttaccgtgct taaacactac gccgatgacg
    11101 atattaccgt ccacaagctg gccgatttca acgttaattt cgcccacggc gaaaactatc
    11161 gcctgttaat ggccgaacat gatatgagct tattcaaagt cgtcgtgcag gccgtagggc
    11221 gagtcgagcg tcgcgacact ctattgaaaa cagaaatctt tttaccccgc gatgtgttcc
    11281 gtaatgttgc atttcagttc gccgctctta gtgaagatag cggtaacgag gtggtatcag
    11341 aaagtatgtc tttgcttaac caccgactca tggaggagtg cgaaaagctg agtcagggcc
    11401 agtcattcaa taatgcggaa cagcgactga cgtttgagca agctatcgtc gcgaatggtc
    11461 gccgcatcga tgaaattcac aaacgtgtcc ttaaaaccga ctggattaat aaggtacgcg
    11521 ctggcaatct cgattatctc gagatatgta atttattccg cgatcctgac tcctttaccg
    11581 atccccagcg ctggctggca aaactccagg ctaatccctt gtataccgcc aatcgacaaa
    11641 tgcaatctgt tcacgacgct ctgtttatcg atcgtcagca agggaatcaa acgattttac
    11701 tttgccacaa acgcggcccg gatggacttg cccaccgaga ttattccgcc ctgtcggatt
    11761 tcgctggcgg cgcaagagag taccggccag agctcaccct ctttccgcag tatagaaacg
    11821 atgtcgattt tacccccggc aacctggtcg gcgagttgat tcgtgaatgt gacaacatcc
    11881 aggaaaaggc attcaaaaaa tgggtaccca accccaagct agttccgttg ctcaaaggca
    11941 atgtcggtga atatctcttc gataaagtgc taaaaagtta tggtgttacc ccactctccg
    12001 accagcaggt gtttgaacgc cttgaaccgc tggtctatga gttttttgac cgctttattg
    12061 aagtgggcga cgacctgctc tgcatcgacg ttaagcgctg ggcgacacag ttggacgatt
    12121 tgacgcgggc agaagaaacg cttgagaaaa gcgacaacaa gattcgccag atccgtaata
    12181 tcgccagcca aaaggcggat actgaggggc agaaacagct ccagacggcg ctggcaggcc
    12241 gttatgaacg tattcgattt atctatctga acgtcgccta cagccagaac cctaataatc
    12301 tgatgtggca ggataatgtg gatcacacga tccactacct caacctgttg caaactgact
    12361 accagtatta tcagcccaaa aatcgagaga gcggacgcgc tcaggaaaac tcgaaactgc
    12421 gcatgacatt ggatataaac ccaatgttac taaccctgct gggtgtagaa aagttgccga
    12481 ctaaaggaaa agtatcatga tccctaatct gaatgagctg acggatactc cgattgcccg
    12541 taccaatttg atcaagcttg aagaagatca gctgacaaca atccagcgtc tattggcccc
    12601 ggtatctaat atctatacga tagactttat ggttcagcac tttactaaag agcgaaaaga
    12661 aaaatccgct gattactatg cgcgaattca tcaggaggta aaaacttgcg tgcggcagaa
    12721 gcttgggctt gaggccggac aggaagtaaa atatgagctg cattgcttac ccaattacca
    12781 tcacgtcttt tttttcctgg cgcctgctgc tgcaccgaac agcctagcgc atcggacttt
    12841 ggcagaacgc attgaaacgc tttgccagcg actcacagct gaaaattatg atttatctcg
    12901 cctgattcag ggattgttca gtctgcattt gaaaatggta atgctggaac aagccagcga
    12961 gcgcttttcg gtaccgccaa cctacttcaa ctctacgttc tatctcaacg ctcgcctgag
    13021 tcagcccgtc acgcagaaaa gcggcactgg agtgatggag gcattcgaac tcgacattta
    13081 tgcatcagaa tataacgaac tcgcctttac cctgcacaaa cgaaaatttc tggtcgaacc
    13141 ggaggatgaa ttgcatctct ctctggacga tacctgcgtg tggtttaaca tcgataatcg
    13201 tcggctcaaa gcccggcgca aactcgatgc ccgggatagc aaactggact tttttcgtga
    13261 gcgcagcggc tatggtgaat gccaggccta tacctataac gtggtcatga atgccgcctg
    13321 cgagcggctc agtgaactag agatcccgca tcagcctatc gcatttcagg ccacccacga
    13381 ggtcaatcag ttcgctaccg acctcgatca acaactgact aatacgctgt tggtggttaa
    13441 taacggcgtc gaatttagcg ccacgcaaga agcttatttc tttgacacat tagccatcca
    13501 gttccccggg tatcaactct ggcctctggc gtcgcttaaa cattctcagc aaaccggctt
    13561 ttctgagctg cctgccagta catctattct ggtactcaat gcagtagatg aagagcggag
    13621 caacagcatc cgccagcaag ataatgaatc tgttgagtac aatgatttct atgcggcctt
    13681 tgccgacgcc cgaaaacaac ccgaactcaa ttgggatact tatacccagc ttaaactaga
    13741 tcgtttgcaa gggtggctaa atcagcaacc tctgcccgta gtcttacagg gtatgaatat
    13801 tgatcacaag ttgttggatg cgattgattt tattaatgaa caattgacaa gcaaccctac
    13861 tcaatacgaa atcgatctta cgaagcctca cagtcgtctc aagtcagcag ttaccttact
    13921 taacagtaag gttcgccgaa caaaaaccga gctatggttc aaagagagct tactcaatca
    13981 gcatcacatc ccactaccag atttggcgga cgggcactat accgcctatg cagtacgcaa
    14041 aacgaaaagc tatctccccc tgcttggata tgtcgaacta aaaatagaac acggccaact
    14101 tagggtggtt gataccggga tcgctgaagg taaattagac tatctgtctg ttgatccccc
    14161 ctctctggga cgattaaaga aattattcga caaaagcttc tatctctacg accacacagc
    14221 agatgtcctg cttaccacct acaacagctc ccgcgtaccg cgcctgattg gcccggcgca
    14281 atttaatatc gtcgattcat acgcttatca ggaacaagaa aaaactctgg cagagcgtaa
    14341 aggggataaa tttaacgggt acgccatcac ccgctctgca aaaccggatc aaaacgtact
    14401 gccctatctg atatcacctg gccgctcgaa atacgactcg ctgaccaaag cgcaaaagat
    14461 gaagcatcac catatttatc tgcaaccgca tgagaatggt gtatttgttc tggtaagcga
    14521 tgcccagcct acaaatccta ctattgcacg gcctaacctg gtggaaaatc tgctgatatg
    14581 ggatgcccaa ggcaaagccg tagatgtatt tagccacccg ttaactggcg tttatctcaa
    14641 tagctttacc ctggatatgc tcaggagcgg tgaaagcagc aagtgttcga tttttgccaa
    14701 gcttgcccgg ttgatggtag agaactagcg gaaaatttag ggcggtgttt ttagaattcg
    14761 ttatgtgtga acctaactga tctcccccct gaaaacagta ccagtctaaa ctgaagtctc
    14821 cggtctttct tcctgctcac agagaggctt attaccatga aaaagacccg ttataccgaa
    14881 gaacagattg cgtttgcgct gaaacaggcc gaaaccggca cccgcgtcgg ggaagtctgc
    14941 agaaagatgg gtatttctga ggccaccttt tacaactgga agaagaaatt tgccggtctg
    15001 ggcgtgacgg aactgcggcg tctgcggcaa ctggaggatg aaaatcagcg gctgaagaaa
    15061 ctggtggctg atctgagtct ggataaggag atgttgcagg aggtactgaa acaaaagttc
    15121 tgaggccggc tcagaagcgt caggcggtac attttctgcg ggaagcttac cgtatcagtg
    15181 tccgacgggg atgtggactg ctgatacaga gcagaaccgt ttaccactgg cagagtcggc
    15241 gagatgatcg ggcgataacc ctgcgcatca gggagatagc ggaaacccgg atccgctacg
    15301 gttgcccgcg tattcatatc cagttacggc aggaaggctg gcctgttaac cacaagaaaa
    15361 cacaccggat ttattgtctg gaaggactta acctgcggag aaaacgtccg tgcaggcatg
    15421 tcagtgcagc acatcgccaa cagcgtccca tcctgaccgg tgtcgatcag tgctggagta
    15481 tggatttcgt gtcggataat ctgtttaacg gacggcgttt ccgggcgctg actgtagtcg
    15541 ataattttag tcgggagtgt ctggcgatcc atgccggaaa gtcgctgaaa ggcgaggatg
    15601 tggtcggtgt gatggaaggg ttgcgggtgc cgggtaagcg cctgccggta cgtattcaga
    15661 cagataacgg cagcgagttt atctcaaaaa gtctggataa atgggcgtat gaacacggag
    15721 tgaccatgga cttctcgcgc cccgggaagc cgacagataa cccgtttatt gaatcattta
    15781 acggcagtct acgggatgag tgtctgaaca ttcactggtt cctgtcactg gaagatgcgc
    15841 aggacaaact cgacagctgg cgcagggaat acaatcatga aaggacgcat tcatcattaa
    15901 atgacatgac tccggctgaa ttcatccgaa gtctccggaa agacgaagat ctctgattta
    15961 gcactgcact gattttgggc cagggtcata actccacaca taacgaagcc ctccacttaa
    16021 cattgatttt tcgtgataac cctcacaaaa ccaaagcgat tttttatggg ggctttagtg
    16081 ggggcgctgt ttacaagaaa tcgcaacaaa accaataaca aacagtatgt taatcgtgga
    16141 tatgattccg agtccgggca ccactaattc ttaagaaccc gcccacaagg cgggtttttg
    16201 cttttggatc tgacaataac cttcacgaaa aaaattagct tataaagtct gggtgaatta
    16261 ctctcgccac gttaacgaga gtaattttat tgatattaat ctcctgatac tttacccccg
    16321 tccaaactcc agccgctgca cattcaccat cccaggcttc tcagcagcac tgacatcaat
    16381 ttgtgtcacc cgcagcgcat atttttcatc cagtgcgttt aaccatttca gcaggtcatt
    16441 aaacaccaca ggttctatcc agacctgaat attctcccca cgctcggcta tccgcctgat
    16501 gaccaccgag tgcgcggaag cactgtcact gatgacccgc gatacctgcg caggcgttgt
    16561 tgtggcagat tttcgcgctg caataatatc cggcgcggcg ttcttcagtc gggtgttcat
    16621 tgccaccagc tgctgcaaca tcgtctcctg ttgttctatc cgttcgctca acggctgcca
    16681 gatgaggacg taatatccgg cgctaaacag gaacactgcc gcagccagta acatgccttt
    16741 ttcacgcggc gaacgccccg ccaggtgttg tgccaaccag tgttcgccgc ggcttaactg
    16801 gcgttcacgc cattgctgaa aatagtgaat aaatttatcg cgtaacatgt tatttcctcc
    16861 gcaacgttac gccgccagaa accgcatcac cctctttctg taacgcgtcc tgttgcacaa
    16921 caaaatctgc cgccagcgcg gtacgcagtt tgtcgaagct ggcaaagttc gcggcccgta
    16981 gctggaggtg aagtgtctgg cgtttttgat caaaggtgaa accacgcatc tcaatgtcgg
    17041 aaagtgacgc tgatttcagg atactggcaa tcgctgacaa ctctgagagc agccgggtat
    17101 cgtcagtctg tgggcgatat tttttcagcg ccatcgtcac ctgagagcgt aaattcacaa
    17161 tccgcttctg ctccgggaac agcgttaaga actgtttctc agcctgggcg cggctttgcg
    17221 ccacctgttc gctgacgctc cataacgtca cgccccgttc cactgccagc gcaaccagaa
    17281 tcagcaatat cggcagaatc atcactcgcc agcgcgccca ctgttttcgg tagctgacgc
    17341 gaggctgcca cggccctgtt agcaggttcc cttccggttc gccataagtg gtaatggctg
    17401 gcagagccgt aacggtcagg cgttcagcgt ctgcgccagc ccatgctgat agttcttccg
    17461 gtgcaatgcc gactacagtt agcgaaagcg gtaaatcctg ttcattgagc tgggcgcgga
    17521 acatgaccgg agccagcgcc agcccggcgc tccatccacg gcattcatcg atgcggcaga
    17581 taacccgttg tgcatcgcca gccatataac cacaaggcat ggacatccag tccggtgcaa
    17641 cggtggcgcg ggtgatgccg tttgcctgca accactgcgc aatgttgcgc atatgctgtt
    17701 ggtgaatcac tgccacggtt gccagttgct ggtcgatttt caacggggcg aaatgcagtt
    17761 catcgatatc ctggttcagc tcttcttcca gtaaggcggg cagaatagtc ggtatctgct
    17821 tgcgggcaac atcaggcagt tcaacctgcc atacgctgat ccattcgccg ggaatgtaga
    17881 gtcgaatcgc atcagtttgc agccattgtt ggagacgttc gtcagcaacg tcaggccaga
    17941 taccgtgctc cacgtcggga gtacggcgct gccaacggat gggattagcg gcgcaaagtg
    18001 ggaaaaaaat ctcaaggatg gaactcactc actttctcct gtctgatgcc agataacaga
    18061 gaagtgctgt gggcccatgc ggacaattaa cgaattcatc gtcagttcaa tctcattcac
    18121 ggtgatatct gaacgcagcc agaagtaatt gctgtccacg ctcaggacgg tttttagctg
    18181 ttttttagta cgctcatcga cgtcagcaag tagcggctgt gcaagaaact gatcgacatc
    18241 ttcccagccc ttcgccggac gttgttgtaa taacgcccgc gcctgaacag ggcttaacca
    18301 cgggtcaaac agcgcctcaa gaatcacact ttgcgtgacg tctaaagtat tgatgttgat
    18361 ttgctggcgg gtcatcggca gcgcacagac cagcggtttc agtttttgat aaagcccggc
    18421 gtccattccc tgcaccacgc gcatctcgct gatatcggcc agcggttgat tggcggcgta
    18481 gaaaggcacc gagcgggcga gatattcgct gtcttcacgg cccagacgcg tctgcacgct
    18541 gcggtcttca tcaataaact cccacaggct ttcggctatc agttcggccc gataagcagg
    18601 cacatcgagg cgcgagatca gggcaatcag ttgttgtaac gcgagcggac gcgacgccgt
    18661 cgtcggctga gcgaggacat tcaggttaaa gcacgcctgt gcgtcacgca gagtgacggc
    18721 gatttgcccc tgcggcagtg ggaaaaaacg agggccggaa gcccacggct gtgccagatg
    18781 cacgcgcttt tcatttttca ggctcagact gagtgcgctc aacgccagac tttctgcact
    18841 ggcgctatac catagcgcct gctggtactc ctgctggctg cgggttcgcc caagttgttg
    18901 ctgcatccgc ccggaaagcg tgatggttac cagcatcata accgccagca ataccagcac
    18961 cacaaccagt gccattccgc gttttggtgg tgaggtgatc atgataattg cggcccgcgt
    19021 aacaaccaga tgcgttcaat ctcgccccat tgcggcgaat gcagggtcat gcgcaccgcc
    19081 acagggatcg cctgtactga cgaccagttc tcctgccagc gcgtaccatc atagaactgc
    19141 aaacggagcg aatccgccgg gattaatttt tgcattgtcg gcttcacgct gcctgctgcg
    19201 tcggtcagcg gccaggctaa ccgttcgaga taaccgtcat gaatgcggta accgacggtg
    19261 agcagattac tgcgcggcag gcgcatcaat ggattaacca cgccgccgcg cacaaagcgc
    19321 attccttcac tctcagacgc cagcacgcca gcgcctgcca gtaacgcagg ttcacgctgg
    19381 ccctgatcgc ctcttaccgg acgtggcatc atttgggtca gatcgtgggt cagaaaactc
    19441 atcgtttgct gcatgaggtt tagtttttga tcgtgtccgg cgacggcgct attcacgcgc
    19501 gtgacgccgt tagtcacctg ctgcgccatc agtgccagcg aggcaaaaat ggcaattgcc
    19561 accaacattt ccagtaacgt gaaaccagcg cgagtccttc tcactgttgt cctcccacgg
    19621 cgctaaacca ggcgcgtcgt gactgaatta ccgacgaaaa gtcttcgtga cggctgactt
    19681 caatatccac cgcctggagc agcgcgttac ctgtattcag cggtgtggtt cgccagtacc
    19741 agcggtttcc tgccatgtcg ctctcgccct gatgcctgac cgcccgttgt tcacgggtca
    19801 tcaggtagag cagcgcggtc tggttttcag ccacccagcc tgctaccgtt ttctcctcaa
    19861 gaacgtgctg attactcagc gcaccgctgg caatctgtaa caccgccgtg gcagccagcg
    19921 caaaaatcgc cagtgcgagc atcacttcca gcaaggtaaa tccgcgcttc atggcatcct
    19981 ctcatcacat tgactgaggg ataacgcgcc atcgtgtgcc agttttaccg cccaacatgc
    20041 gttttgcgcc gctgagtaga agcgcagcgt aaacggcgtg gcgggttcaa aaggcgaaaa
    20101 acggatctgc ggcgtcttct ttttcgcctc tttttgcaac tccagttcaa tatcgtgcag
    20161 cgtcaggcga cgacgttgca gctccagcgc atattccttc tgccagatat cacttccggg
    20221 ttgtaacagc atctgcacct gtttcggcac cgtaacctgt gccgataacc gggtcgcaga
    20281 aacgggcaac cactgcccct gacgacgttg cataaactgg tatcccggcg ggtcgattag
    20341 tacaccgagt gtctgccctt cgatcactgc cctgtcttta aactgcgcaa agcgagtcag
    20401 aaaatcctgc gccgcttttt tcgcgggcga ttcagaatcg gtcgcaaaag tctgtaccac
    20461 gcccgcactg gcaaggccga taaggaaaat caccagcatg atttccagaa gtgtgaatcc
    20521 gcgttcaggc actgcttact gaaactcctg caaattccag ttaccgatat ctgcgccagc
    20581 accttcccca ttttcctgac catccgcccc cagggtgtaa acatcaaaca gtcctttttc
    20641 acccgggctg agatactgat aatcattgcc ccacggatct tttggcagtc gtttaatgta
    20701 cccaccggta cggtagttgc gggaatccgc catattggct ggctgctgga tcagcgcctc
    20761 aagcccctgc tcggtagttg gataacgccc gttatccagt cggtacatat ccagtgcgtt
    20821 ttccagcgcc acgatatcgc tgatggcttt ttgccgatcc gctttctctt tattgcccaa
    20881 caggttaggc accaccagac ttgccaggac gccaagaata acaatcacca ccatcacttc
    20941 cagcagggta aaacctgccc gtggtttttg tgtgcgggat aacgaattca tgtgataact
    21001 ccgtaaatta cattccaacc atattgttca gttgcagcat cggctcgagg atggcgatga
    21061 cgataaacag caccacgccc gccatcatca ccaccagcgc cggttcaaac agccctaacg
    21121 ccagccccac ctgggtatca aattcccgtt cctggttgac cgctgcctgc tcaagcatgg
    21181 tttccagttc gccgctctgt tcgccggagg cgatcatgta cagcatcatc ggtgggaaca
    21241 ggcgcaactc cgccagtgca gcgcgcagac tgcttccttc gcggacgcga tctgccgcca
    21301 gcagcagttg ttgttcgaca tagcgatttg ccgacacggc ggcagcggtc tgaatgcctt
    21361 ccagaagcgg aacactgctg gcggtgagga tgcttaacgt gcgggaaaag cgcgccgtat
    21421 tcagtccgcg agcgacccgc cctatcacag gcaggcgcag caaggtttta tcccagcgca
    21481 gatgcatcgc aggatttttg agtagccgtt gccccagcac cagaagcccc agcaatcccg
    21541 ccagccagta aacgccgctg gtctgtaacg tgtcgctcat agcgatgagc attcgggtgg
    21601 aagcaggcag cgcgtgtccg aggtgatcga actgttcgat aattttcggc accactgccg
    21661 tcagcaaaat agtgaccacg cccgttgcca ccaccagcaa taccagcggg taaagcatgg
    21721 cctgcagcag gcgtgatttc aggcgctgtc gctgttcggt gtaatccgcc aggcgattaa
    21781 gcaccacgtc gagatgcacg gatttttctc cggcagcaac catcgaacaa aacagagaat
    21841 caaagacgcg gggatgttcg cgcaggctgt ccgacagggt ataaccttcc tgaatccggc
    21901 tgcgcagcgc cattccgagg cttttcacat gcagtttttc actttgctca ctgaccgcct
    21961 gtaagcaggt ttcaagcggc attgctgcct gtaccagcgt tgccagctgg cgcgtgaaca
    22021 gcgccagatc tgccgccgcc acgcgacgat gtgcgtgccg ccgacgctgc aacattcccc
    22081 ctgccgatgc attcatccgc gtttcaatat gcacaggaat gagatcttta ccgcgcaata
    22141 gctgacgggc atgacgtgcg gaatccgcct caatcatgcc tttggttttg cgaccattac
    22201 gctccagcgc ctgatagtaa aacagtgcca ttacgcctcc atggttaccc gcagaacttc
    22261 atcgagagag gtttctccgg cgagcacttt ctcaatgccg ttgctgcgga tacccgcaga
    22321 gcgttgccgg acataacgtt ccagctccag ttccccggcc tgacggtgga tcaaatcacg
    22381 caatgtggcg tctaccacga tcagctcatg gatggcagta cgtccgcgga aacctttgtg
    22441 attacaggcg gggcagccct gcggatggta cagagtgacg gtacgcacgt cggtaattcc
    22501 cagcaggcgt ttttcttcgt cagtggcagg cgcgggctgg cggcagtcgg ggcacagcgt
    22561 gcggaccagt cgctgcgcca tcacgcccgt cagactggaa gagagcagga aaggctccac
    22621 gcccatatcc tgcaaacgcg tgatcgcccc caccgccgtg ttggtatgca gcgtggaaag
    22681 caccagatgc ccggtaagcg acgcctgaac agcgatttct gcggtttcgg tatcgcggat
    22741 ttcaccgacc atcaccacat ccgggtcctg acgcaaaatc gcgcgcaggc cacgggcgaa
    22801 ggtcatgccg acgcgagtgt taacctgcgt ctgacctatc ccttcaatca tgtattcgat
    22861 ggggtcttca accgtgagaa tgttgcgcga gtggttgttc agctcctgca atccggcata
    22921 cagcgtggtg cttttgccgg aacccgtcgg ccccgtcacc agaaagatgc cgtgcggttt
    22981 gtgtaacagc tggcgcaact gcgcggtcag ttgctgactc aaccccagac gctccagcgt
    23041 caggcgggcc tgatttttgt ccagcagtcg cagcaccacg cgctcacccc aggcggaagg
    23101 catggtggag acacgcacgt caatcgcccg gccgcccagc agcagcgcaa tacgtccatc
    23161 ctgcggtacg cgcttttcgg cgatatccag ccgtgccatc accttaatac gcgataccag
    23221 cagagaggcc agtttgcgcc cagggcgcag catttcatgt aatgtgccgt caacgcgaaa
    23281 acggatcacc agactctttt caaacgtctc gatgtggata tccgaagcgc cttctttgat
    23341 tgcctctgcc agcatggcgt tgatcagttt gatgatcggc gcatcgtcgt cactttccag
    23401 cagatcttcc gtttctggca gttcttccgc gagggtaaaa aagtcctcag cagaaccgag
    23461 atcttccatc agctggcgag cctcggaaga gtcccgctgc cagaccgcat tcagccgctg
    23521 ttcaaattcg gcctcgtcga tttgccgcag cgtaaagggc gcgttcagcc cccgctgcag
    23581 ctcctgcaaa acagagagcg acaacggatg aacatggagg atctccagcg acgcttcgca
    23641 ccatgccacc aggctaaacc gacggctgaa actgtagggc agacgcacgg tgttagcggt
    23701 ggtttcctgt gctacaggca ccattaacgc gttctcccgg cattgaggaa cgcgcgtact
    23761 tccggcggta aggcctggtt ttgcgctggc agtaccggct gcgcggtgtg cggcatcagg
    23821 cttaagcctt gttcatcgcg atagatctgc tcggcgcgca tatagttata tttgcgctgc
    23881 gacacgccgt ctgccgccat accgtcacgc agaatggtcg ggcggataaa caccatcagg
    23941 ttacgttttt cttttttatc cgccgtcgat ttaaacaggt taccaatcaa tgggatatcg
    24001 cccagcagcg gcactttcgc cacactttct cccgcctggt cgtccatcag accgccaagt
    24061 acgatcagtt caccatcgtt agccagcacg gtggtcttca gtttgcgctc accaaacacc
    24121 acgtcgaggc tggtctgtcc ttccaccttc gagacttcct gctcaatcac catctgtacc
    24181 gcgttacctt cgttaatctg cggcgtgact ttcagcatga tgccgacttt tttcctttct
    24241 actgtattga aaggattgct gttattggag ccaacggtag agccggttaa taccggaaca
    24301 tcctgaccca ccatgaagaa ggcttcctgg ttgtccagcg tggtgatgct cggcgtggag
    24361 agtacgttcg agctggagtc gtttttaacc gcctgtacca gcgccatcca gtcgcctttc
    24421 accacgccaa ccgccgtacc gctaaagccg gaaagaagct gtgcgagcgt ggagagatcg
    24481 ccgttggtat ccggattaat ggtggtagcg ccgttttcac tgatcaccgt cgagcctttc
    24541 tgcggtttcg cctgagaaat ggctgccccc agcgtaccga tagggatctg cgtaccatta
    24601 gcaaactgca ttaatccggc atctttcgac gcccactgca cgccgaagtt gatattactg
    24661 ccttcggcca cttccacgat caacgcctcg acgtgcacct gagcacggcg aatatccagt
    24721 tgttcaatca cgctttgcag cgactgcatg atgtcctgcg gcgcagtaac aatcagggca
    24781 ttactgtgtt tgctggcggc gatggagaca acctcacgcc cgctaccgac ggtgccttcc
    24841 gcctcttctt tagccgctgt gagcgtgccg ctgacctgct tcagcacatc gaccagatct
    24901 tccgctttgc tgtatttgag atagaaaacc tggctgttgc cgctgcgctc catttctgag
    24961 tccagccgac ggatcaggcg gcgcattttg tcccgcgtgg ccgggtcacc actgacaatc
    25021 acactgttgg tgcgttcgtc ggcgacaatt tgagatttca gcgttgctgg ctggttctca
    25081 ccgctgtttt tggtcaggct ttccagcaca cgggcgattt ccgaggccga ggcgttatcc
    25141 agcgggatca cctcttcagt gcgatttccc gcgtgatcca cgcgctggat cacttccgtc
    25201 aggcgttcca cgacggaggc gcgtccggtg agcataatca cgttggaggg atcgtaatta
    25261 acaacgttgc ctgagcctgc gctgtcgatc atctggcgca gaatcggtgc cagctcgcgt
    25321 accgacacat tacgtaccgg caccactttg gtgaccattt catcgcccgc gtagttgtcg
    25381 ctgccttcac cgaccagcgg cagcggttca acttttgcgg cgctggattt caccaccttc
    25441 agcacgtcgt tttccatcgg tacgacggca tacccctgcg cttccagcag gttaaggaat
    25501 agctggtaat actggcgttc attgagcggg gtcatggtgc gaatactcac tttcccctga
    25561 acgcccggcc ccataatgat ggttttatta aggttagcgc cgacggtttc gatgaacgat
    25621 ttcaggtcgg tatctttaaa attagcggtg aaagtggctt cttccgccca gaccggtgaa
    25681 ctgcataatg ccgctgccag caccagcggc agtaaacgct tttttgtttt gaggccagtt
    25741 gtcttcttac gccagaccga caacgtaata tcacgccaaa acacgatgaa tgattctcct
    25801 ggattaaatg cggttagcgc agcgcgatgg aaatatcgtg gcgcgcaccc ttgcgtaaaa
    25861 ccgtaagttg aatggaatcc attgaaggta actgccgcat cagagcaatc attgctcgtg
    25921 gatcagtgaa atcctgctga tttagcgcaa tggcgatatc gccttcctta aaaccgctgg
    25981 catcgaacag agaacgatct gcccccggct tcactgcata accgacaatt ccctccttac
    26041 gcacaggcgt aagctggata tagttaaaaa ttttctgcgg atctttcgcc agtgcctgac
    26101 gcacggcagt cgggatctca actggcgcac tgacagcagg ctcagcaaca gcttgcttta
    26161 cttcgtcact gatggctttt ttgttggtca cggctacggt ggaacgctcc tcttcaacca
    26221 ggctcaggcg ctctattttt ccctgatagc gcagcatcac atggtcgcgg ttgatttcct
    26281 caatcaccgc gttgtgagaa ccaagccgtt ccccctgcaa atagacctgc tgtttgccac
    26341 cttcttcaat aaccgcgccg ggtctggcac caaaggcgat cccgcgcagc accacattaa
    26401 gacgcgtttc ggccacaggt gcaggttcgg gttgttttac cggtgcggcg acgggctgat
    26461 atttgccaaa ccagttttgc tggctgatta attgcacgtc atttttatcg aacgtttttg
    26521 catccgtacg gggcggttta ttcaccgatg gagaaaccac cgtatattcc gcagaaaagg
    26581 agaaataacg ccagagtgaa tgcgccactt ttgcagaaat aataagcagc atcagccaga
    26641 acatcccgcg tgcaattttt cgcagatgct ctttgtctgt attaagagat tgtctctggt
    26701 taagagtgtg acgaattttt gtcagccatt gaatgagata aattcgtgcg tcacgaaaaa
    26761 caacccgcgc caatttatat ctacccgacg ttatgctttg actattccac aggtggtacg
    26821 atccagtttc cgctgatact ggttacctgt acgtgtatca ttaatcgtta tgctgtaatt
    26881 aattccctca gtgagcatta atttcaccgt tgggtcagtg cacatttgtc gttgatagct
    26941 ggttaaaaag gcgtctggcg tctgcgtggt ctgagtaccc gcttcgctga taatggtcat
    27001 ttttaccgtc gtgccgctac tttgtgccag cactaaggta tatcccgcag atttaatcgg
    27061 caggttttgg ctgatatttt gcgcctgttt tttcgccagt aaactggcgt tttcattatg
    27121 gctggcgcag ccacttaata atcctgtagc gctcaacaat agcgatatta ataccctccc
    27181 tggcatctgt tttatcgaca tggtaaataa tctctaaggt tattaataag agttaaaatg
    27241 tcactttgat aatgacgtgg ttatcattaa aacaatgcct gtagataaag tgttgctata
    27301 ccgcccagac ttaaacacgg tccaaaaggc agtgtggttg atcctctttt tgtaataacg
    27361 gcatatatca ggccgcagca tgaggcgatt aaagcaacat tgggcagtga taacggtccc
    27421 acccaactac ctaaagcggc gaacaataat acatcgccca tgcctaatgc ttctttacgc
    27481 agaactattc cggctatcca gcgcagagag taaaaagtga taaaccccac caggacgccg
    27541 gtgactgcat cttgtagcgt gagcgggctc tgctgcgccc atgccgcaat tagtcccgtc
    27601 cacaatacgc cctgagtaaa aacatcgggc agccattggt gatcgaggtc gatgatgctc
    27661 gcggcaatca gccaggcgga taatatcatc accgccagcg cccatccact ttccggccac
    27721 accaggctcg ccagcaaaaa agcgagtgcc gtcaataact ccaccagcgg ataacgcttg
    27781 ctgattttcg cctgacaatc acggcagcgc cctttgagca tcagccagga gagcagcgga
    27841 atattgtcac gtatccggat ggtctgctga caatgtgggc agtgcgaacg cggtagcgca
    27901 aggcttattt ttgactgcgc actcggcatt tcaccatgaa actccgccat ttgttggcgc
    27961 agcatgatgg ggtaacgcca aatcaccaca ttcaaaaaac tgccgatgat caatcctccg
    28021 acggtagcca gtatgggcat cgccacgggg tattgctgaa aaacatcaaa aagcatggtt
    28081 aaaggttgtt tgttgtaact tgccggatgc ggcgtaaaac gccttatccg gcctacaggt
    28141 atctgccaac gcaagtaatc gcggctttcc ctgaagaccc gataagatgg cgcatcgggc
    28201 atatgtgtta acccgatgcg ccttatttca tgccggatgc ggcgcgagcg ccttatccgg
    28261 cctacagatg tctgccaacg caaataaacg cagctttccc tctctgtagg cccgataagc
    28321 ttgcgcatcg ggcatatgtg ttgacccggt gcgccttatt tcatgccgga tgcggcgcga
    28381 gcgccttatc cggcatacag gcttactcgg ctgacatctt atgctcggta acctgattaa
    28441 tggtttccgg cccttgctgc ggtttcggca gaccaagcgc cgccagcgtc tcgtaagccg
    28501 actggctcac accgccctcg aagttcatct cgctcacccc cggcagctgg taagcattcg
    28561 cgcccggatt ccatttttta aagaactccg aaagatccgt ctgggcgacc caggaggcac
    28621 acagcatcag cttgtcggca gcgttaccgt tggattcggc acagtagttt ctgttgccaa
    28681 atttgtcatt gccaacatca tcgccgcgtg ctttacgatg catcaactgg aacaggttcc
    28741 agcctttcat cccttcacgc tcgctgtaaa actctggcag aggagtgcca tctggatacc
    28801 atttcttgat atcaaagttt ttctctgccc attccttcag ctgcgcgtac atcagcaaac
    28861 ggtcacccgc accgccgcgc gcccaagcct ggttgttgct ctcctccaga tattccggcg
    28921 cgacggtaat atcgtcagcg acacggttca tcttgccgag ataacgatcc tgcatgtata
    28981 gcgccagcac gttgttcgcc acttcggtcg cgcctggaac agtcagcggc gtttctgcgg
    29041 cgttgtgccc tacttcgtgc cagatcaacc agtcgttcag cggcgtcgtc ggcagcgtgg
    29101 tgctgttcgt cgagaagctg ctgttcatta ccggataacc cgagtgcgca tcaccgatgg
    29161 agatctgcac atcgtttgtg aaacgatgtt tatgtcccgt caatgcttta taggtaaaca
    29221 tccggtgctt accgctcgtt tcatcacgac cgtagaagtc attcatcgag ctggcaaagg
    29281 tgtccagatc gttagcgaat tgctccaatc cgccagtgta attgctggca ttcaggttct
    29341 tcttcggtgt ggtatagacg aaagcgtctg attccagctc acccagcgga gccggtgagt
    29401 tcagatcgtt tttccatgcg ccgtctttat agaacggtgc ttttaccacg ccagtaaagg
    29461 tgaagctggc agattcattg gtagagctat tgcccttgat ataaatcagg ccaccgtaag
    29521 gcaccttgaa cttcaccgta ccgctagcgt ccagagagta cgttttagtc actcttggcg
    29581 gacggttcag cgcaacttca tgcttctcac gtccggtcag gtcgtcagcc agcgccacgg
    29641 tgacggtcac aggaacgttc gcattggact taatggtgac ctctttctga gccggtgccc
    29701 acaggccggt tgattgcatg ttacccgcaa accatttggt cggattcgag tacaggctga
    29761 tggtttcagt aacgttctgt ccctcttccg atactgctcc cggatacttc tccacatcaa
    29821 ccttaatgtt cagatcccac caggaacggc ccagcatcag gcgcgtcagc ggtttttcca
    29881 tatagttgag tggatagctt gggttcatca tgcccgcttt accgctactt tcaccgtaga
    29941 tcatgttgtt atcgaccagc gattttttca gatctgcgga gcactgtgtg ccatcggtat
    30001 aggcatcatt ggtatagcag ttcaggaact cggtgaacgt tttaaagccc agctcgtcat
    30061 ttttgccttc ttcataacga tagctcgtat cgttccacag ccagaccgac atgttctggt
    30121 acagacgttc cagatcgacg ctgctcagac gctcaccttt gcgaccattg gtacggaagt
    30181 aaagctcgtg ctgatacaga cgctgaatgt tggtgcctaa atccgccgcc tgcaccatcg
    30241 ctttcgcagt atcggcgctg agattcagtt gcgtatactg cggaacatac atgccaccgg
    30301 ttaccggaac tttcgtacca ggacggtatt ccagacagtt gatttcgtag tgatatgtcg
    30361 agtccttaca ctcctgtaat ccaggaaact tctcaaagat ttttgccttt gcagcctcca
    30421 gagactcttt tgttttaaac tccgcctcat caataaaggc ataacgcgtt acctgtttgc
    30481 cctcaacttc ctcctgccag ctggcaactt ccagtttcgg tttgtcatca ggtttgtttt
    30541 ctacctgata tttccactta acttcccctg tcttactatc gatggtgtac ggcagcgcac
    30601 cgtctacggc aggataacgt tcatagaccc aaatgcccgt tgcgcgctgc tgacgaacgc
    30661 ggttcggata cccttgagga tcgttattca ccaccgactt gttcagtgcc attgacagac
    30721 ctgcagcatc caacagacgc acaaaaccag acgcgctctc ttccttaaga ttgctcatca
    30781 cgttttccat gatcagcacc gatccacctt tgttcatata ggcgatcaaa tcggtcacat
    30841 cctgctgagt cagcttcggt ttgctggtat ctgcacgcag cgggattgca taaggatcat
    30901 taccaacctg agtcacatac tcaaagccgt tgaggatcag cagcggcatt tcctgcggat
    30961 cgagatcgcc atagctactt aaatgctcaa cagagatgcc cgcaaaatcc ggatggaagt
    31021 cgaacgcagc gctgtttcct gtaacctgac catgacgttt aaaatagaca gtatccaggt
    31081 tggtgcctac ggtcatgctg gctttcgcgt ccggcgtcca tttatcgtcg gacagatagc
    31141 gcagtacgtt ctgcatgaag tgcttcatgt catctgaatc accgctgagc gtacactgtc
    31201 cgtctttatt aacgcccccg ttccagctgt aaccgttcgg gcaacgcaaa atgctgttgt
    31261 agtgtgggtt accgataacc atcagcttgc catcgcccac ttgccccagc gaaataaacg
    31321 gcaggttaaa ggtggcggta tcgcgcgtaa cgttttccgg ctcaacaaga gaaggcgctt
    31381 ccgtaatgta cgccagctcg tttttatccc aggcgcgttt ttcgccaaac gccagccagt
    31441 agtttttatc attacgcgcc atcagaattg ggaatgccgc gttggagata ttcaccactg
    31501 cctgaccgcg cgcattaccg gtgctgccat agaagttggt agagtcatgg aatacatgga
    31561 acttgctgac agatttgtag ttcgtatcca cgccccacag cttgttgata acgccctgaa
    31621 tctggccgtc attaacattg cgcgtcgtca gcgagaacca gcgagcctcg ttacaaccgt
    31681 cggttttcgc acaaatcgcg gtatcgatct cattggcctg acccgtctta aactgctcga
    31741 taaattcgtt aggcagatta acgacttgct caccttcccc cagcgtcgca ccgttggata
    31801 acgagagatt gataatctcg ttgatcacgt tgggatattc ggcaaagacc ttgcgtacat
    31861 cgtccggaac aacacgggta ttattttgcc cggtcgtcga atagcgatga ataagctgat
    31921 cgatattcgc cccgcgaact tcatcaccca gttcagtcag cgcaatggtc gacttattgc
    31981 cgcgcactga acccagttca aaggtatcga taccaaagga gatggtttcg ccccagctaa
    32041 aggaaaattc accattttcc cctgtcacgc cacggcctga attggtgtag tagttgacgc
    32101 cagcaacacc atatccctgg ctatccacca gtcggccttc ggagagaatg atttccgaag
    32161 gttgatactg ataaaactgt tccgcgttag ccgacacgaa ggaggcgttc agatccggtt
    32221 ttgttcccgg agtcgtgaca gggactaccg gtgaagtatg agtggatggc gctttatcag
    32281 tcgcggcatt gttttccacc tcttcattga ccagcttttt gaactcttcc ggtgccagat
    32341 cgatttgctt atacagcgag tcgaaacgtt tactctcgat caccgaggag aactccaggc
    32401 acacctgttc tgtattcgcc gggcaactgt tcatggaggt gaccagcgag agcgcattgc
    32461 ttttcttgtt gtcggaaccc gccagttctt gcgcgtcctc aagactaaac gacacttttt
    32521 caaccgcacg caggctacgc gcagcttctg actgggtgtc gaaggtagca attgtcgtgc
    32581 tgttccctgc cacacaggta actttgtcgc ctggtgtaaa ggtaaagcca tcgctggatt
    32641 caccattaca ggtagcacca gttacccgct ggcttccgcc cagggtcaga taacctgttt
    32701 tcgtaggaac aggctctggc tccggttctg gctctggatc aggtgtcggt tccggcgtag
    32761 gttctgggtc cggcgttggc tcaggcgtcg gctccgggtt tggtgttgga tcaggtttca
    32821 cttccggcaa agaccctgtt ccagaatcta caggcggcgt atcggaggaa gatccggaac
    32881 caccgccatc acaaccggct aacagggttg cgctcaaaat agccgctaaa agcg
//
LOCUS       DAZUCE010000025        32735 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZUCE010000025 DAZUCE010000000
VERSION     DAZUCE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.025"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(205..2526)
                     /gene="yhgF"
                     /locus_tag="ACHY03_001073"
     CDS             complement(205..2526)
                     /gene="yhgF"
                     /locus_tag="ACHY03_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFS3904333.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2623..3099)
                     /gene="greB"
                     /locus_tag="ACHY03_001074"
     CDS             complement(2623..3099)
                     /gene="greB"
                     /locus_tag="ACHY03_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFS3904334.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3327..4046
                     /gene="ompR"
                     /locus_tag="ACHY03_001075"
     CDS             3327..4046
                     /gene="ompR"
                     /locus_tag="ACHY03_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFS3904335.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            4043..5395
                     /gene="envZ"
                     /locus_tag="ACHY03_001076"
     CDS             4043..5395
                     /gene="envZ"
                     /locus_tag="ACHY03_001076"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFS3904336.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5472..7094)
                     /gene="pckA"
                     /locus_tag="ACHY03_001077"
     CDS             complement(5472..7094)
                     /gene="pckA"
                     /locus_tag="ACHY03_001077"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFS3904337.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7473..9197
                     /gene="yhgE"
                     /locus_tag="ACHY03_001078"
     CDS             7473..9197
                     /gene="yhgE"
                     /locus_tag="ACHY03_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFS3904338.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWFAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAILLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILQVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            complement(9260..10138)
                     /gene="hslO"
                     /locus_tag="ACHY03_001079"
     CDS             complement(9260..10138)
                     /gene="hslO"
                     /locus_tag="ACHY03_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFS3904339.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10163..10564)
                     /gene="hslR"
                     /locus_tag="ACHY03_001080"
     CDS             complement(10163..10564)
                     /gene="hslR"
                     /locus_tag="ACHY03_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFS3904340.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10575..11243)
                     /gene="yrfG"
                     /locus_tag="ACHY03_001081"
     CDS             complement(10575..11243)
                     /gene="yrfG"
                     /locus_tag="ACHY03_001081"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFS3904341.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGVAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11308..13443)
                     /gene="igaA"
                     /locus_tag="ACHY03_001082"
     CDS             complement(11308..13443)
                     /gene="igaA"
                     /locus_tag="ACHY03_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFS3904342.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNTESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTIELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPTHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            13763..14323
                     /gene="nudE"
                     /locus_tag="ACHY03_001083"
     CDS             13763..14323
                     /gene="nudE"
                     /locus_tag="ACHY03_001083"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFS3904343.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14488..17040)
                     /gene="mrcA"
                     /locus_tag="ACHY03_001084"
     CDS             complement(14488..17040)
                     /gene="mrcA"
                     /locus_tag="ACHY03_001084"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFS3904344.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDVWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17160..17939
                     /gene="hofM"
                     /locus_tag="ACHY03_001085"
     CDS             17160..17939
                     /gene="hofM"
                     /locus_tag="ACHY03_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFS3904345.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            17939..18478
                     /gene="hofN"
                     /locus_tag="ACHY03_001086"
     CDS             17939..18478
                     /gene="hofN"
                     /locus_tag="ACHY03_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFS3904346.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18462..18902
                     /gene="hofO"
                     /locus_tag="ACHY03_001087"
     CDS             18462..18902
                     /gene="hofO"
                     /locus_tag="ACHY03_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFS3904347.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVFWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18892..19296
                     /gene="hofP"
                     /locus_tag="ACHY03_001088"
     CDS             18892..19296
                     /gene="hofP"
                     /locus_tag="ACHY03_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFS3904348.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELIQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTQRTGGKAAKSDADGG"
     gene            19208..20446
                     /gene="hofQ"
                     /locus_tag="ACHY03_001089"
     CDS             19208..20446
                     /gene="hofQ"
                     /locus_tag="ACHY03_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFS3904349.1"
                     /translation="MKQWIAALLLMLIPSVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            20847..21368
                     /gene="aroK"
                     /locus_tag="ACHY03_001090"
     CDS             20847..21368
                     /gene="aroK"
                     /locus_tag="ACHY03_001090"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFS3904350.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21425..22513
                     /gene="aroB"
                     /locus_tag="ACHY03_001091"
     CDS             21425..22513
                     /gene="aroB"
                     /locus_tag="ACHY03_001091"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFS3904351.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22605..23891
                     /gene="damX"
                     /locus_tag="ACHY03_001092"
     CDS             22605..23891
                     /gene="damX"
                     /locus_tag="ACHY03_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFS3904352.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPVPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPAASTKAPAATSTPAPKETAATAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23998..24834
                     /gene="dam"
                     /locus_tag="ACHY03_001093"
     CDS             23998..24834
                     /gene="dam"
                     /locus_tag="ACHY03_001093"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFS3904353.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24852..25529
                     /gene="rpe"
                     /locus_tag="ACHY03_001094"
     CDS             24852..25529
                     /gene="rpe"
                     /locus_tag="ACHY03_001094"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFS3904354.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25522..26280
                     /gene="gph"
                     /locus_tag="ACHY03_001095"
     CDS             25522..26280
                     /gene="gph"
                     /locus_tag="ACHY03_001095"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFS3904355.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            26273..27277
                     /gene="trpS"
                     /locus_tag="ACHY03_001096"
     CDS             26273..27277
                     /gene="trpS"
                     /locus_tag="ACHY03_001096"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFS3904356.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(27408..28139)
                     /gene="frlR"
                     /locus_tag="ACHY03_001097"
                     /gene_synonym="yhfR"
     CDS             complement(27408..28139)
                     /gene="frlR"
                     /locus_tag="ACHY03_001097"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFS3904357.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYLAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(28239..29024)
                     /gene="frlD"
                     /locus_tag="ACHY03_001098"
     CDS             complement(28239..29024)
                     /gene="frlD"
                     /locus_tag="ACHY03_001098"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFS3904358.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIKPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPELVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(29024..29851)
                     /gene="frlC"
                     /locus_tag="ACHY03_001099"
     CDS             complement(29024..29851)
                     /gene="frlC"
                     /locus_tag="ACHY03_001099"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFS3904359.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDE"
     gene            complement(29901..30923)
                     /gene="frlB"
                     /locus_tag="ACHY03_001100"
     CDS             complement(29901..30923)
                     /gene="frlB"
                     /locus_tag="ACHY03_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFS3904360.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGEQASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(30944..32281)
                     /gene="frlA"
                     /locus_tag="ACHY03_001101"
     CDS             complement(30944..32281)
                     /gene="frlA"
                     /locus_tag="ACHY03_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFS3904361.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPSFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(32577..>32735)
                     /gene="yhfL"
                     /locus_tag="ACHY03_001102"
     CDS             complement(32577..>32735)
                     /gene="yhfL"
                     /locus_tag="ACHY03_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFS3904362.1"
                     /translation="FIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKII
                     GGCGPTAQ"
BASE COUNT         7589 a         8406 c         8900 g         7840 t
ORIGIN      
        1 gctaatgttt aattaaggtt tcagttgtta atgaagtgat aaatattaac gaaatatatt
       61 gtgagtttgg ctgtggaata aatatgccgg agatactctc cggcactttt atgtcaggca
      121 aggcgttcgt tgccagatgc ggcgtgaacg ccttatctgc cctacgattt cgtgcaaatt
      181 cgaaccgtag gccagtccgg gcgtttaacg ttttttaccc atcgctgccg ccagcgcgtc
      241 catcatcgcg ctattaccgg caggctgcgc ttcacgaccg cgtggtttgg ctgccgggcg
      301 gttgttttgc gggcgatcgt taccgccgcc gcgacgagag ttggtttcgc caggctgctc
      361 gtccagacgc atagtcaggg cgatacgttt acgctgaaga tccacttcca gcactttcac
      421 cttcacaatg tcgcccgctt tcaccacggt atgcggatct tccacaaact tgttcgacaa
      481 tgaagagatg tgaaccaggc cgtcctgatg cacgccgata tcgacaaacg cgccaaagtt
      541 ggtgacgttg gtgacagcgc cttcgaggat catacccggt tgcaggtcgt tcattgtctc
      601 gacgccatcg gcaaactgag cggttttaaa ttccggacgc ggatcgcgac ccggtttttc
      661 cagctcttta atgatgtcag ttactgtcgg cacaccgaat ttttcatcag taaagtcaga
      721 cgctttcagg ttacgcagtt cgctgctgtt acccatcaga tctttcagtg cctgctgtgt
      781 tgctgccaga atgcgttcca ccaccggata ggcttccggg tgaacggtag acgcgtccag
      841 cgggttatca ccgtggttaa tgcgcaagaa gcccgcgcac tgctcgaagg ctttcggccc
      901 cagacggctc acttttaaca gttgctgacg gttctggaac tggccgttct catcgcgcca
      961 ggctacgatg ttttgcgcca tcatgcgcgt caggcccgcc acgcgggtta acagcggaac
     1021 agaagcagtg ttgagatcga cgccaacggc gtttacgcag tcttctacta ctgcgtccag
     1081 tttgcgggcc agttgcgtct ggctaacgtc atgctgatac tgacctacgc cgatagattt
     1141 cggatcgatt ttcaccagct ccgccagcgg atcctgcaaa cggcgggcga tagaaaccgc
     1201 gccacgcagc gaaacgtcga gatccgggaa ctcctgcgct gccagctcgg aagccgagta
     1261 aaccgacgcg ccagcttcgc tgacgatcac tttctgtgcg gtcactttcg ggaactgctt
     1321 ctgcacgtcg agatagaaac gttcggtttc gcgggaagct gtaccgttgc cgatcgccac
     1381 cagttcaacg ttatgctttt cacacagcgc agcaacagtc atcgctgctt ttgcggcttg
     1441 tccggtgtgc gggtaaatgg tatcggtcgc taccagtttg ccagtggcat cgaccaccgc
     1501 cacttttacc ccagtacgca gacccggatc gaggcccatc gttgcacgca gtccggcagg
     1561 ggccgccatc agcagatcgt gcaggttacg ggcaaagacg ttgattgctt catcttccgc
     1621 gcgttcgcgc acggtgccca tcagttcggt ttccagatgc atcagcacct tgatgcgcca
     1681 ggtccagctc accacgcctt tgcgccagct atccgccgga gcattgttca ggcgcaggcc
     1741 gagatgatcc atgatgattt gctcgcaata gctctctttt ggcggctcat cgaactgcgg
     1801 atcggcattc agcgaaagct ggagtacgcc ttcgttacgc ccacggaaca tcgccagcgc
     1861 gcggtgagaa ggcaccgttg acagcggttc gtgatgatcg aaatagtcgc ggaatttcgc
     1921 cccttcctct tctttaccgc tcaccaccgt agaaaccaaa tgcgcgttct tccacagata
     1981 atcacgcact ttcgccagca gcgcggcatc ttcggcaaac cgttccatca ggatatagcg
     2041 cgcgccgtcc agcgcggctt tggtatctgc cacgccttta tctgcatcaa tatattgcgc
     2101 agcggcgact tctggcgtgt gtgacggatc gctccacagc aggtcagcca acggctcaag
     2161 ccctgcttca atggcgattt gcccgcgggt gcggcgttta ggtttgtagg gcaggtagag
     2221 atcttcgagt tcggttttgc ttaaggtggc gttgatggcc ttcgccagat catcggtgag
     2281 tttgccttgc tcggaaatgg acttgaggat cgcctgacgt ctctcttcca gctcgcgcag
     2341 atagctcaga cgcgtttcca gattacgcag ctgcgtgtca tccagaccgc cggtgatttc
     2401 cttacgataa cgtgcaataa aaggcacggt attcccttcg tcaagcaggc gaacggcagc
     2461 gtcaacctgt tccgggcgcg cctgaatttc acccgcaata atgcggcaga acgaatcatt
     2521 catcatggtt tgagttcatc ttttcggatc aaaaatcagg ggatagttat acggactggc
     2581 tggcaaaaat gccagccatc ggcaggaggt taagactctt ccttacggtt tcacgtactc
     2641 gatagcatta acataccagc tcgcttcccc ggcaggagta ttcaccacag ccagatcgcc
     2701 gacttctttt ttcagcaatg cgcgggccat cggggaatcg atagagatgt aatctttacg
     2761 gccaaaaatt tcatcgtagc cgacaatacg gaaacggtga gtcacgccat cgtcgttttc
     2821 aatctccacc cacgcgccaa aaaagacttt gccttcctgc tgaggggagt aatcgacgat
     2881 tttgagattt tccatgcatt tagtgagata gcgcacgcga cggtcgattt cacgcagacg
     2941 ctttttatta tactgatagt cagcattttc gctgcggtcg cccagacttg cggcccaggt
     3001 cacctttttt gtgacctccg ggcgttcttc acgccagaga taattaagct cttgtttgag
     3061 tttttcatac ccttcccggg taaccagggg cgttttcatc tcgttgattc cctttgtctg
     3121 tttgataatg cgcacattgg gtataacgtg atcatatcaa cagaatcaat aatgtttcgc
     3181 cgaataaatt gtatacttaa gctgctgttt aatatgcttt gtaacaattt aggctgaaat
     3241 tcataccaga tttagctggt gacgaacgtg agctttttta agaatacacg cttacaaatt
     3301 gttgcgaacc tttgggagta caaacaatgc aagagaacta caagattctg gtggtcgatg
     3361 acgacatgcg cctgcgcgcg ctgctggagc gttatctcac cgaacaaggc ttccaggttc
     3421 gaagcgtcgc taatgcagaa cagatggatc gcctgctgac tcgtgaatct ttccatctta
     3481 tggtactgga tttgatgtta cctggtgaag atggcttgtc gatttgccga cgtcttcgta
     3541 gtcagagcaa cccgatgccg atcattatgg tgacggcgaa aggggaagaa gtggaccgta
     3601 tcgttggcct ggagattggc gctgacgatt acattccaaa accgtttaac ccgcgtgaac
     3661 tgctggcccg tatccgtgcg gtgctgcgtc gtcaggcgaa cgagctacca ggcgcaccgt
     3721 cacaggaaga ggcggtaatt gctttcggta agttcaaact taacctcggt acgcgcgaaa
     3781 tgttccgcga agacgagccg atgccgctca ccagcggtga gtttgcggta ctgaaggcac
     3841 tggtcagcca tccgcgtgag ccgctctccc gcgataagct gatgaacctt gcccgtggtc
     3901 gtgaatattc cgcaatggaa cgctccatcg acgtgcagat ttcgcgtctg cgccgcatgg
     3961 tggaagaaga tccagcgcat ccgcgttaca ttcagaccgt ttggggcctg ggctacgtct
     4021 ttgtaccgga cggctctaaa gcatgaggcg attgcgcttc tcgccacgaa gttcatttgc
     4081 ccgtacgtta ttgctcatcg tcaccttgct gttcgccagc ctggtgacga cttatctggt
     4141 ggtgctgaac ttcgcgattt tgccgagcct ccagcagttt aataaagtcc tcgcgtacga
     4201 agtgcgtatg ttgatgaccg acaaactgca actggaggac ggcacgcagt tggttgtgcc
     4261 tcccgctttc cgtcgggaga tctaccgtga gctggggatc tctctctact ccaacgaggc
     4321 tgccgaagag gcaggtctgc gttgggcgca acactatgaa ttcttaagcc atcagatggc
     4381 gcagcaactg ggcggcccga cggaagtgcg cgttgaggtc aacaaaagtt cgcctgtcgt
     4441 ctggctgaaa acctggctgt cgcccaatat ctgggtacgc gtgccgctga ccgaaattca
     4501 tcagggcgac ttttctccgt tattccgcta tacgctggcg ataatgctgt tggcgatagg
     4561 cggggcgtgg ctgtttattc gtattcagaa ccgcccgctg gtcgatctcg aacatgctgc
     4621 tttgcaggtc ggtaaaggga ttattccgcc gccgctgcgt gagtatggcg cgtccgaggt
     4681 gcgttccgtt acccgcgcct ttaaccatat ggcggctggt gttaagcaac tggcggatga
     4741 ccgcacgctg ctgatggcgg gggtaagtca cgacttgcgt acaccgctga cgcgtattcg
     4801 cctggcgacc gagatgatga gcgagcagga tggctacctg gcagaatcga tcaataaaga
     4861 tatcgaggag tgcaatgcca tcattgagca gtttatcgac tacctgcgca ccggacagga
     4921 gatgccgatg gaaatggcgg atctcaacgc agtactcggt gaggttattg ctgccgaaag
     4981 tggctatgag cgggaaattg aaaccgcgct ttaccccggc agcattgaag tgaaaatgca
     5041 cccgctgtcg atcaaacgcg cggtggcgaa tatggtggtc aacgccgccc gttacggcaa
     5101 tggctggatc aaagtcagca gcgggacgga gccgaatcgc gcctggttcc aggtggaaga
     5161 tgacggtccg ggaattgcgc cggaacaacg taagcacctt ttccagccgt ttgttcgcgg
     5221 cgacagtgca cgcaccatta gcggcacggg attagggctg gcgattgtgc agcgtatcgt
     5281 ggataaccat aacgggatgc tggagcttgg caccagcgag cggggcgggc tttccattcg
     5341 cgcctggctg cctgtgccgg taacgcgggc gcagggcacg acaaaagaag ggtaaataaa
     5401 acgggaggcg aaggtgcctc ccgttttgct ttctataaga tactggatag atattctcca
     5461 gcttcaaatc attacagttt cggaccagcc gctaccagcg cggcacccgc tggggtgtca
     5521 gtgtatttat cgaagttgtc gataaacagt ttcgccaggg tttcggcttt ttcctgccac
     5581 tgttccgggg aagcgtaggt gttacgcgga tcgagaatct tcgtgtctac gcccggcagt
     5641 tcggttggga tcgccaggtt aaacatcggc agagtgaaag tttctgcgtt atccagcgaa
     5701 ccgttgagga tggcgtcgat aatagcgcgg gtatctttaa tcgagatacg tttgccagtg
     5761 ccgttccagc cagtgttaac cagataagcc tgcgcgcccg ccgcctgcat acgtttcacc
     5821 agcacttctg cgtactgagt cgggtgcagc gacaggaatg ccgcgccgaa gcaagcggag
     5881 aaggttggcg tcggttcggt gatgccacgc tcagtaccgg ccagtttggc ggtgaagcca
     5941 gagaggaagt ggtactgggt ttgatcggca gtcagacgag aaaccggcgg cagcacacca
     6001 aaggcgtctg cggtcaggaa gataactttc gttgcgtggc ccgctttgga aaccggctta
     6061 acgatgttat cgatgtgata gatcggataa gaaacgcggg tgttctcggt ttttgaacca
     6121 tcatcaaagt cgatagtgcc atcttcacgc acggtgacgt tttccagcag cgcatcgcga
     6181 cggatagcgt tgtagatttc aggttccgct tctttcgaca gcttgatggt tttcgcgtag
     6241 cagccgcctt cgaagttaaa cacaccgtca tcgtcccagc cgtgttcgtc atcgccaatc
     6301 aggcgacgtt tcgggtcggt ggaaagggtg gttttaccag tgccggaaag gccgaagaac
     6361 accgcaacat cgcctttctc gccaacgttg gcggagcagt gcatagaagc gatacctttc
     6421 agcggcagca ggtagttcat catcgagaac atccctttct tcatttcgcc gccataccag
     6481 gtgccgccaa tcagctgcat gcgctcggtc aggttaaaag ccacgaagtt ttcggaattc
     6541 agaccctgtt ctttccactg cgggttagtg cacttcgcgc cgttcataac gataaagtct
     6601 ggtttgaaac ctgccagctc ttcatcgctc gggcgaataa acatgttttt gacaaaatgc
     6661 gcctgccagg ccacttcggt gatgaaacgg acggaaagac gagtatccgg gttcgcacca
     6721 cagaaggcgt cgacaacgaa cagacgtttg ccggaaagct gtttggtcac caggcctttc
     6781 agatgctgcc aggtttccgg agagagaggt ttgttatcgt tcttaccttt gcctttgtct
     6841 gcccaccaga aagtatcgcg agtggtatcg tcacggacga tatacttatc ttttggtgaa
     6901 cgaccggtga agatcccggt atcgacggca acggcaccca gattagttaa caccccgcgc
     6961 tcataacctg tcaggctcgg atcgagctct tcctgataca gcaggtcgta gcttgggttg
     7021 taaacgatat catgtacgtc actgatacca taagcctcga gttcttgcgg ggtcaaacca
     7081 ttgttaacgc gcatttcact gctccttagc caatatgtat tgcctgaata gtaaagtctt
     7141 tttgggggtg ttaaccgcga caaggctcat agatttacgt atctggagaa attcatattt
     7201 gacaaaaaac tccgtgattc ctgtcacgaa acggttgcta ttatcgcaga aatagcattt
     7261 taggtgtgga aaatgttctc aaaaggttaa atcttggttt atatggcggg aatattgtga
     7321 gtggaatcgc attcctgaat gaaattgatg caaattcaac cggtaaagta tcgattcacc
     7381 acgctaactt tttttgatta ttttctgaaa aaagcaaaat actactgccc ggatgcgata
     7441 attaacccac cttttgattc tggataacca acatggataa cgttgaactt tcacccgcaa
     7501 cacgctgggg gatgattgct accggattac ttcaggggct ggtttgctac ctgctgatcg
     7561 cctggtttgc cggaaaaaat cacagttgga ttgtttatgg cgtgcctgcg accgtcgcgt
     7621 tttcatccgt tttactcttc tccgtgatct cttttaaaca gaagcgcctt tggggatggc
     7681 tggcgctggt gtttattgcc acgctgggga tgagcggttg gctgaagtgg caaactgatg
     7741 gcatgaaccc ctggagagct gaaaaggcgc tttgggattt tggctgctat ctgctgctga
     7801 tggcaatatt gttgctaccg tggatacaac aaagccttcg tatccgcaat gacagtagcc
     7861 gctatcgcta tttttaccag tcagtatggc ataacgtact tatattacag gttatttttc
     7921 tcgccaatgg cctgacgtgg ctggtgcttt tactgtgggg tgagttgttt aaacttgttg
     7981 gcatcacgtt tttcaaaacg ctcttttttg caaccgactg gtttatttat ctcacgttag
     8041 gtctggttac cgcgctggcg gtgatcctcg cgcgaacaca gtcacgttta atcgactcta
     8101 ttcaaaagtt gttcacgtta atcgccacgg ggttgctgcc gttagtatca ttgctaaccc
     8161 tgatgtttat catcaccctg ccgtttacgg gcctgagcgc gatttctcgc cacatctccg
     8221 ccgctgggtt gctgttaacg ctggcctttt tgcaattgat cttaatggct attgtccgcg
     8281 atccgcaaaa agcgtcactt ccctggacgg ggccgttgcg ttgcctgatt aaaaccgctt
     8341 tgctggttgc tccgctgtat gtgttcgtcg ccgcctgggc gttatggctg cgggtcgctc
     8401 agtacggctg gactgtcgac cgcttgcagg gcgcgctggc ggtgctggtg ttactggtat
     8461 ggtcgctggg gtattttgtc agtatcgtat ggcgtaaagg gcaaaatccc cttgttcttc
     8521 agggcaaagt gaaccttgcg gtttcgttat tggtgttggt aatactggtg cttcttaatt
     8581 ctccggtgct ggacagtatg cgcattagtg tgaacagtca tatggcgcgt tatcagagcg
     8641 gcaaaaacac gccagaccag gtaactatct acatgctcga acagagcggt cgctatggac
     8701 gtgcggcgct tgagtcgctg aaaagcgatg ccgagtatat gaaagacccg aaacgtgcgc
     8761 gggatctgtt gatggcgtta gatggagagc aacatcttca gcaacagata tcggaaaaag
     8821 tattagccga aaatgtgtta attgcccctg gttctggtaa accggatgcg acattctggt
     8881 cggccttaat ccaggatcgc tataacgtga tgacctgtat tgaaaaagac gcctgcgtac
     8941 tggtcgagca agatctgaat agtgatggca gggcagagcg gatcctcttt gcctttgatg
     9001 atgaaagata cattgtctat ggctttgatc ctgacaagaa agaatggcaa gagcttacga
     9061 tgagtttact tccgcgtgat ataacgaaag aaaaattact cacagctgcg aaggatggaa
     9121 aactgggaac gaagcctaaa gcgtggcgcg atcttgtagt ggatggtgaa aggctggatg
     9181 tgaatctgaa tgagtgacaa agcgcatcag gtgtattagt cgtaaaatcc ggcagagccc
     9241 tctctgccgg acatactcat taatgaactt gcggatctgc cggagacgcg ttgttgcgga
     9301 tctcggcaat atccatcgca ttgaacagat agtggttacc gcagtaatca caatgcatgt
     9361 caatttcgcc atcttccgcc aggatgctat cgacttcttc atcaggcagc gttttcagcg
     9421 catcggcgca acgttcacgc gagcaggtgc atttgaattc cacatcctgc ggatcgtaaa
     9481 ccgtcacctc ttcttcgtga tacaaacgcc acaacacttc gtttgccggt aaggtcagca
     9541 gttcttcggt tttgatggtt tcggttagcg tcgccaggtg gtcaaagtcg tcctgctggg
     9601 cattttgcgc aggcattacc tgcaacaaca taccgcctgc agccggtttg ccgtctacgt
     9661 cgccggtgcg aataaacagg cgcgtcggca gctgttcaga acgcataaag taatcttcca
     9721 ggcaggccgc cagggtatca ccttccagac ccaccacgcc ctgatagcgt tcgccttcgc
     9781 tcggggtgat ggtgatcacc acgtaaccat tgccgaccag cgttttcagg tcggcatttt
     9841 ctggaatttc gccctgcacg cgcgccacac cgcgcatctg ctggttgttg ttaccgttaa
     9901 ttaccgccag attcatcgga ccgtcgcctt gcagctgtac ggtgatatca ccatcaaact
     9961 tcagcgtagc ggttaacagg ctagtcgcaa ccagcagttc tgccagcacg tttttaacgg
    10021 gctgcggata atcgtgattc tcaaggatct gttgcagggt ttccgaaacg gttaccagtt
    10081 cgccgcgcac ggcaaagttt tcaaacagat agcgatgtaa ttggtcatgt tgcggcataa
    10141 tcatctctct tgcaggtgac agttattcac tgtcgccgtg tttaaatcgt aacaggtcgc
    10201 ggcgctcttt tttgtccggg cgtcggtccg ggtgcggcat ggttaaggca ttaagtttac
    10261 gtgccagcgc cattttttcg cgtttctcta cactttccgc agtctcttca tacagcaagg
    10321 ctgcctcgct ggcggggcga cgctgttcag taatcgcctt tacaatcacc gtgcgttcgt
    10381 catttccctg gcgcagagtg agcgtggcat tcagctcgac gattttgctc ggcttgctgc
    10441 gctgcccgtt gtaatgcacc ttaccgcctt caatcatttc acgggccagc gcgcgggttt
    10501 tataaaaacg ggcagcccat agccatttat ccagtcgaac ctcaacagca ggtttttctt
    10561 tcatggcgtc tccttcacat tagcgagggg atcaggcggc ggtagtcatt cagtgacgga
    10621 tggcgttgat actgtttctc ggcaacccct gagtcaggat tagtcacgcc gaggcagtaa
    10681 cgaataccaa attgcgcggc agcatcgaga atcgcttcgc tgtcatcaat aaacagcgtt
    10741 ctttcagctt tcagacccgt agcttcggcc accgcatgcc ataaccgctg atcctctttc
    10801 ggataaccaa atgtgtgggt ggaaagtaat aaatcaaggt gtgcgtccag accggtatgc
    10861 tcaagtttta ccgccaggtt gtgcggatgc gcattggtga gcaaaattcg ctgcttaccg
    10921 ctggctttca gtgcctcaag aaacggaatg gtatcttcac gcagtacggc acgcggtccc
    10981 atctcggtgg tcatcgcaca gatatccaga cccagttgct cactccagta atcaagacag
    11041 taccagttta gcgtatgctg tacgtcgtga tattgctggc gcatatattc catcgcttcc
    11101 tgtggcgtaa ccccgttttt cgcgccccat gtttcaggca ccagcttttg ccagaaatag
    11161 ttatcgaagg cgaggtcgag caacgtgccg tccatatcca gcagaacggt atctacgtcc
    11221 tgccaggcaa tgttgatatg catgaggaaa atctccagag tgaagcaatt tgcgcgacag
    11281 ggtagcataa cctgccgcgc aaacgtgtta ttcgataagg ctttctgaag gggtgatcag
    11341 ttgcgggttc aggcagcttt cataataggc ctgaatcttc atcatgcgag tgcgatgacg
    11401 ctggtaacgc cgccaggcct gtacgccatt gtaaatggcg ctacccagca tcgtcagtag
    11461 cagcagcgtg gtgctgagat agcgccacag gccggaacga tccgggatcg gatgaaggcc
    11521 tatatgctgc gtaccattgg cgtcagtgaa gatttttgtg acgataccct cggcgttaaa
    11581 cggcgtatgc atcagcattt gtgccagttt ctggaaagcg ttccactgtt cttgcggcgg
    11641 gtagtcgtaa agtgatgccg aaggccaggg ctgatcaaca aaatcgctgc cttcgtcgct
    11701 gacaatcagg aatccgccgg gggccggact gtttagtgat tgtgccgctc gcgccgtttc
    11761 atgcgtgata aacggcgcgg tggaggttgc caccaggtta tccagcgatt ccgcactcac
    11821 cgggcgtaat aacacattca cgccatcgag cttcccggcg ttggcgcgtt ttaccagcgc
    11881 gtcccagtct ttactgttgc cgagattgac cagcgcattt ttcagtcgca cacagtcatc
    11941 tttggcagaa cataaatccg ctgtcttcag tacaatgtcg ccaaaatcat caagcaatac
    12001 catgccggat ttttgaatcg ctgaacgtaa tgaggcactg acgcgagatt catcttccgg
    12061 tttagggtgc agctggcgat taactgcttc agtcaatgcc gtcgctttgt tgaccagctc
    12121 agattctggt aatggtaatg agcgggcgtc attccagatg atctgcgagc agtcaaacgg
    12181 taaaaaaggt gaattggttt tcgcgctcca ggttccggaa gtgcgaatat tacacattcc
    12241 cgtaccgcta atacgcaatg tatcgcctac ccgcacacca gcgtcagcca gttgttttac
    12301 gctggtggct tcaatggtct gcgcgccttt catccatgag agagtgaatt tcagcggcat
    12361 atccagcggg atccagaata acagcataaa cagcaccagc agcgagcccg cagcgataat
    12421 cgtactgcgc agccagtgct gtaacggaaa gttttttact tcatcatgca gcgaaagata
    12481 tcgcccctga cgcactacgt ggcggtcgag atagatatca atatcggttt gttggccgag
    12541 atcctgagca atgtatggct gccagtgtgt gggatagacc aggtcgataa taccgagtga
    12601 aatattgttg atctgttcct gatcgttttc gccaaacaat ccccaacgac ggggtgtacc
    12661 gcgcagacaa tgaatttccc gcagggagga ttttgccggg ggggcgaata gcccccacag
    12721 accagcgccc agcagcagta acgcgccgcc tgccagccac ggaacaaata catccggggt
    12781 gatcaggcag aaaaagaaca tcaggaagga ggcgacaatc agcaacgctt cgcgcagccc
    12841 gcgcggacga ctcagcgcat actcttcgtg cgtttctttg cgaatattga gtagctcgat
    12901 ttgctcactc tcttccccgc gaatcgacgc ctgtgttgac ggaacaggtt gcaaggcata
    12961 gccgcgcgtt tcctgcatgt actcctgcag cgtatgaccg ttgagtgaaa taaccaacgg
    13021 cagcgaatcg gtatgaatca gttcaatggt attctcatcg ttgatgtact gttcccagaa
    13081 ggggggcagg tggacttcta ccgaatcgag gtagtaacgc catttattcg gatcgtcggt
    13141 agagatgccg taacgcgtga tagcgtgtgt cagcatcatg acgttgttgc tttcggtatt
    13201 cagcgccaga gagatcggtg ccgcgctggc tcccgttggg ccaggtacct gtaatacctg
    13261 cgtcaggctc tcaagataat tttcaacggc gctacgttct tcaggtgtga gtttacgcgt
    13321 ttgcgcatcc gcgaaggcgt tggtccaggg cagctgacgc cgtctggatc gcacttttat
    13381 cagccatccc gcaagtagtg agcaggccag caaagcagct aaaaaaatca caatggtgct
    13441 catgctttcc ccatcttact tagtctgtca ggcgtggtca gtcgcaccgg acacaataca
    13501 tgattctgcg gttggctatc ggcaagcgtg gagtttaact tgagcatcat taagcacatc
    13561 acagtaaccg ctgatcatag caaaccctct gaaggcggaa tatcagcaaa ttcctggagt
    13621 tatttcaacc tattgacttt taatttgatt tgaggacgat tatttattaa gttacataaa
    13681 tgtaaataac atgcaattca cattgccgaa agcgtgtccg atatcgcaca ataaccgctg
    13741 atttcacagc atagatccca cgatgagcaa atcattacaa aaacccacca ttctgaatgt
    13801 tgaaactgta gcccgttccc gactgtttac cgtcgagagc gtggatctgg agttcagcaa
    13861 tggcgtgcgg cgtgtttatg aacgaatgcg tccaaccaac cgggaagcag tgatgattgt
    13921 gccgattgtg gacgatcacc tgatcctgat ccgcgaatat gcagtgggaa ctgaatccta
    13981 cgaattaggt ttttcgaaag gattaattga tccgggtgaa agcgtctacg aagccgctaa
    14041 ccgcgagcta aaagaagagg ttggatttgg tgcgaacgat ctgacttttt tgaagaagct
    14101 cagcatggca ccgtcttact tttccagcaa aatgaatatc gtggtagcgc aagatctcta
    14161 cccggaatcg ctggaaggcg atgagccaga gccgctacca caggtgcgct ggccgctggc
    14221 gcatatgatg gatttgctgg aagaccctga cttcaacgaa gcgcgtaatg tcagcgcgct
    14281 gttcctcgtg cgcgaatggt tgaaagggca ggggcgagtg taaatgcgtg aagtttgcct
    14341 gatgcacaac gcttatcagg cttatggggt tcctgcaata tattgagttt tcacgatttt
    14401 gttagccgga taacgcgttc acccgtatcc ggcataaaca agtgcacttt gtcagcaaac
    14461 tgaaaaggcg ccgaagcgcc tttttaatca gaacaattcc tgtgcctcgc cattatcgat
    14521 aatggtcgtt cccacctcgt gcaccgcctg ttgtgtcggc tgcgtacctt cgatgaaata
    14581 ctcttcgcgg ctgttgccac cattagctaa ctgcccggtg ctgcgatcga tattcaccgt
    14641 cacaataccc ggcggcggcg tcagcggctg ctccggcaca ccttcaagaa cggctttcat
    14701 ataagcgtcc catgcaggct gggcactctt ggcaccgcct tcgtaacctg agatctgatc
    14761 tttaatcgct ccggaagccg ttgtatgacc gagattacga cggtgatcat caaagccaat
    14821 ccagaccgag gtcacaacgc ccggaccgta acccgagaac cacgcatctt tcgaactgtt
    14881 agtggtaccg gttttcccgc cgatatcgcg acgctgcaaa tcacgacctg cacgccagcc
    14941 ggtaccctgc cagcctggct caccaaagat attggtgttc aaagcactct taatcaggaa
    15001 tgccagcgga gtgttgatga cgtgcggtgc gtactcctgc gcgccagtct tcgcaactaa
    15061 cgcctgattt gcctgctcca gctgcggcat tggtacagaa acattctgct gctcgcggga
    15121 gatagcgaca tcttcaacat cgttattttc cagcacgttc gatttctgcg tatcaccgta
    15181 aatcaccgga atatcacatt ccgggcaagc tactttcggt ttcgcttcga aaatcacgcc
    15241 gccctgatcg ttttcaattt tgctgataaa ccacgggtcc accaggaagc cgccgttcgc
    15301 catgaccgcg tagccacgcg ccacctgcat tggggtgaag gacgctgaac ccagcgccag
    15361 cgattcggtg tggacaatgt tttgcgccgg gaagccgaag cgttgcagat attctgcagc
    15421 gtagtcgacg cccatcgccc gcattgcgcg taccatcacc acgtttttcg actgacccag
    15481 cccctgacgt aagcgaattg gaccggcata ctgcggtggt gagttcttcg gctgccagtc
    15541 agaaccggca cctgcgtccc agcgagaaat tggcacatcg ttcaacatac ttgccagcgt
    15601 cagaccttta tccatcgccg cggtgtagag gaacggtttg atgttggaac ccacctgacg
    15661 cagtgcctgg gtggcgcggt taaacttgct ctgattaaaa tcaaagccac cgaccaacgc
    15721 cataacggca ccgttttgcg gattgatcga caccagtgcc gagttcactt ccggcacttg
    15781 tgccagccac catacatcgc caacctgacg aacccagatt tgctgacccg tttgcagaac
    15841 atcggtcact ttacgcggcg tcggtccctg ctgagtatcc gaacggtaag gacgcgccca
    15901 gcgaacgcct tccatactca atgcgacggt cgacccgtcc gccagcattg ccgtcgcttc
    15961 ctgaggattg gcgctggtga ctgcggcagg cagcagcgga ccataggtcg gcagcgcctt
    16021 cagtgtatcg gtaatcttgt tgttatccca cgccgactcg cccactttcc acagcacatt
    16081 tgccgggccg cgatagccgt ggcgcatgtc gtagtccagc acgttattac gtaccgcctg
    16141 ctgtgcggcc tgctgcactt tgcgggtgat ggtggtgtaa atgcgataac cgtcttcata
    16201 ggcactttcg ccataacggt tatacatctc ctggcgcacc atttcgctca ggtacggcgc
    16261 agagaaagca atctccggcg cgtgatagtt agcgttaatc gcctcagtgc gtgtctgatc
    16321 gaactgttgt tgggtgatat acccttcatc cagcatccgc gatagcacga cgttacgccg
    16381 cgcgacggca cgatccatcg agtagagcgg gttgaaagtg gaaggcgctt tcggcagccc
    16441 ggctatcaca gccatttcgt tcagcgtcag ttggtcgacc gttttcccga aatagacttg
    16501 tgccgcagca ccgacaccat aggcgcggta accgaggtaa atcttgttca gataaagctc
    16561 gaggatttcg tctttcgtca gcaactgttc aatgcgaatc gcgaggaaga cttccttaat
    16621 cttacgcatc agcgtgcgtt ctggactgag gaagaagttt ctcgccagct gctgggtaat
    16681 ggtacttgcc ccttgtgacg cgtggccgga gaacagcgcc acacttgccg cacggaagat
    16741 ccccaccggg tcaacgccgt gatgctcgta gaagcggctg tcttccgtcg cgataaaggc
    16801 tttcaccatc tccggcggga tttgatccag cgtaaccgga atacgacgtt tctcaccgta
    16861 ttgagcaatc agctcgccat cggcgctgta aatctgcatc ggaatttgca ggcgaacatc
    16921 ttttaatgtc gccacatccg gcagttgtgg ctcgatgtag cggtataggc cataaatcga
    16981 gcctgctccc agcagaatgc aacagactgc aaggatcaaa aaatacttta cgaacttcac
    17041 tggaaatttc ccatttagtt tcatttgggc agtttataaa caaacgcgcg gtagtataaa
    17101 ggcaagccag acgcattgat atacccgtca gagtgacggg tgataaggag atcatcacaa
    17161 tggcatttaa gatctggcaa attggtttgc atttacaaca gcaagaagcg gtagcggttg
    17221 ctatcgtgcg gggcgcaaaa gaatgctttt tgcaacgctg gtggcggttg ccgctggcgc
    17281 acgacattat caaagatggg cggattgttg atgcgcagcg gctggctaaa acgttgttac
    17341 cgtggagtcg cgaactgccg cagcgccatc acatcatgtt ggcgtttccc gccagtcgca
    17401 cattacagcg gtcatttccg cgcccgtcga tgtccctcgg tgagcgggag caaacggcct
    17461 ggctgtccgg gacgatggct cgcgagctgg atatggatcc ggactccctg cgcttcgatt
    17521 acagcgaaga ctcactcagt cccgcctata acgtgactgc cgcgcaaagc aaagagctgg
    17581 caacgctgct tactctggca gaaaggttgc gtgttcatgt tagtgcgatc accccggatg
    17641 ccagtgcatt acagcgattc ctgccttttt taccttctca tcagcaatgt ctggcctggc
    17701 gtgataacga acagtggctg tgggcgacac gctatcgctg ggggcgcaaa ctggcggtgg
    17761 ggatgactag cgcgaaggag ctggcggcag cgttatccgt tgatcccgaa agcgtcgcga
    17821 tatgtggcga aggcggattt gatccctggg aggccgtttc tgttcgtcag ccgccgttac
    17881 cgccgccagg tggagacttt gccatcgcgc tggggctggc gcttgggaag gcgtactgat
    17941 gaacccgcca attaattttt tgccctggcg acagcaacgc cggaccgctt ttcttcgttt
    18001 ctggttgctg atgttcgttg cgcctctgct gctggccgtc gggataacgc taatactgcg
    18061 tctgacaagc agcgccgaag ctcgcgtaga cgccgttttg cttcaggggg aacaacaact
    18121 cgcccgcagc ttacagataa cgaaaccacg tttgctggag cgccaacaaa ttcgtgagca
    18181 gcgtttgcaa aggcagcgcc agcgacaatt tacccgcgac tggcaatctg cgctggaagc
    18241 actggcggcg cttttacccg agcacgcctg gctgacaacg ataagctggc agcagggaac
    18301 gctggagatc aaggggctga caacaagcat taccgcgtta aacgcactag aaacgtcact
    18361 ccgccaggat gcatcttttc atctcaatca gcggggggcc acgcagcagg atgcgcaggg
    18421 acgctggcaa tttgagtatc agttaacaag gaaggttagc gatgaacatg ttctttgact
    18481 ggtggttcgc cacatcaccc cgcctccgcc agttttgctg ggcattctgg ttgctgatgt
    18541 tagttacgct catttttctg tcatcgacac accatgaaga gcgcgacgca ttaattcgac
    18601 tacgggcaag tcatcatcag cattgggcca cactgtatcg cctggttgac accactccct
    18661 tcagcgaaga aaaaacgctg cccttttcgc cactggattt tcagttgccc ggcgcacaac
    18721 tggttttctg gcatccatcc gcgcagggag gcgagttggc gttgaaaacg ctgtgggaag
    18781 cagtgccgtc ggcatttaca cggctggcag agcgcaacgt cagcgtgagc cgtttttcat
    18841 taagcgtgga aggtgatgat cttttgttca cgctacaact ggagacgccg catgagggtt
    18901 aaacgctggt tgttggcagg tattgcattg tgccttttaa ccggtatgcg tgaccctttt
    18961 aaaccgccgg aagatctatg ccggattagc gaacttatcc agtggcgcta tcaggggatg
    19021 gtagggcgag gcgagcgcat catcggtgta ataaaagacg ggcaaaagaa atggcgacgg
    19081 gtgcagcaaa acgatgtgct ggaaaacggc tggacaattt tacagctgac gccagacgta
    19141 ctaacgctgg gtaccgggac aaactgcgaa ccgccacaat ggttgtggca acggcaagga
    19201 gatacaaatg aagcaatgga tagccgcact actgttgatg ctgataccca gcgtacaggc
    19261 ggcaaagccg caaaaagtga cgctgatggt ggatgacgtt ccggtagctc aggtgttgca
    19321 ggcgctggct gaacaggaga agttgaacct ggtggtgtcg ccagacgtca gcggtacggt
    19381 gtcgttacat ctaacagatg ttccctggaa gcaggcacta caaactgtag tgaaaagcgc
    19441 cggactgata acgcgccagg agggcaacat tctctcagtg cattccattg cctggcagaa
    19501 tgacaatatc gcccgtcagg aagcggagca ggcgcgggcg caggcaaatc tgccgctgga
    19561 aaatcgcaat attactctgc aatacgccga cgccggagag ctggcgaaag ctggggagaa
    19621 gctactgagt gccaaaggga gtatgaccgt cgataaacgc accaatcgcc ttttgctacg
    19681 agataacaaa acggcgttaa gcgcacttga acagtgggta gcgcaaatgg atctgccggt
    19741 cgggcaggtt gagctgtcgg cgcatattgt caccattaat gaaaaaagtt tgcgtgagtt
    19801 aggcgtgaaa tggacgctgg ccgatgcgca acacgctggt ggcgttgggc aagtcaccac
    19861 gcttggcagc gacctctccg tagcgacggc gacaacgcat gtcggtttta acattgggcg
    19921 catcaacgga cgtttgctgg atcttgagct ttctgcgctc gaacaaaaac agcagctgga
    19981 tattatcgct agtccgcgtc tgctggcctc acatcttcag cctgccagca ttaaacaggg
    20041 gagcgaaatt ccatatcaag tttccagcgg ggaaagtggc gcgacgtcgg tggaatttaa
    20101 agaggccgtc ctggggatgg aggtcacgcc cacggtgtta caaaaaggtc gcatccggct
    20161 gaaattacac atcagccaga acgttccggg gcaggtgcta cagcaggcag atggcgaagt
    20221 gctggcgatt gataagcagg agatcgaaac gcaggtcgag gtcaaaagcg gagaaacgtt
    20281 ggcgctgggc ggcattttta cccgtaaaaa taaatcgggt caggatagcg tgccgttgct
    20341 tggcgacatt ccctggttcg ggcaattatt tcgtcatgac ggaaaagaag atgaacgacg
    20401 cgagttagtg gtgtttatca cgccacgact ggtttccagt gagtaaacag ccgtaaaagc
    20461 ggtaatgttt ttacgctgaa cgtgtttcat ctatttgacg cgcgcaggta tttagcatac
    20521 aaggagtacc gatttgagag ttggtgctct tcgctgcctg cgttccatga tgatgattta
    20581 tcattcaggc ggcattttgc tgtctttttt acgctaatct tacccggtga tttatcgcca
    20641 gagcggtggt agcaaggcag cgcgcttgca gcgaccagat atgcagaggg atgggtgatt
    20701 tattcagttg ccaaacccgc tggagtattg agataatttt cagtctgact ctcgcaatat
    20761 cttatgaggt ttcagttcat gtcctgcggc gctctctgag cgaagcgggt ttatcattaa
    20821 cgaatagtct tagtagtacc gaaaaaatgg cagagaaacg caatatcttt ctggttgggc
    20881 ctatgggtgc cggaaaaagc actattgggc gccagttagc tcaacaactc aatatggaat
    20941 tttacgattc cgatcaagag attgagaaac gaaccggagc tgatgtgggc tgggttttcg
    21001 atttagaagg cgaagaaggc ttccgcgatc gcgaagaaaa ggtcatcaat gagttgaccg
    21061 agaaacaggg tattgtgctg gctactggcg gcggctctgt gaaatcccgt gaaacgcgta
    21121 accgtctttc cgctcgtggc gttgtcgttt atcttgaaac gaccatcgaa aagcaacttg
    21181 cacgcacgca gcgtgataaa aaacgcccgt tgctgcacgt tgaaacaccg ccgcgtgaag
    21241 ttctggaagc gttggccaat gaacgcaatc cgctgtatga agagattgcc gacgtgacca
    21301 ttcgtactga tgatcaaagc gctaaagtgg tcgctaacca gattattcac atgctggaaa
    21361 gcaactaatt ctggctttat atacactcgt ctgcgggtac agtaattaag gtggatgtcg
    21421 cgttatggag aggattgtcg ttactctcgg ggaacgtagt tacccaatta ccatcgcatc
    21481 tggtttgttt aatgaaccag cttcattctt accgctgaaa tcgggcgagc aggtcatgtt
    21541 ggtcaccaac gaaaccctgg ctcctctgta tctcgataag gtccgcggcg tacttgaaca
    21601 ggcgggtgtt aacgtcgata gcgttatcct ccctgacggc gagcagtata aaagcctggc
    21661 tgtactcgat accgtcttta cggcgttgtt acaaaaaccg catggtcgcg atactacgct
    21721 ggtggcgctt ggcggcggcg tagtgggcga tctgaccggc ttcgcggcgg cgagttatca
    21781 gcgcggtgta cgtttcattc aagtcccgac gacgttactg tcgcaggtcg attcctccgt
    21841 tggcggcaaa actgcggtca accatcccct cggtaaaaac atgattggcg cgttctacca
    21901 gcctgcttca gtggtggtgg atctcgactg tctgaaaacg cttcccccgc gtgagttagc
    21961 gtcggggctg gcagaagtca tcaaatacgg cattattctt gacggtgcgt tttttaactg
    22021 gctggaagag aatctggatg cgttgttgcg tctggacggt ccggcaatgg cgtactgtat
    22081 tcgccgttgt tgtgaactga aggcagaagt tgtcgccgcc gacgagcgcg aaaccgggtt
    22141 acgtgcttta ctgaatctgg gacacacctt tggtcatgcc attgaagctg aaatggggta
    22201 tggcaattgg ttacatggtg aagcggtcgc tgcgggtatg gtgatggcgg cgcggacgtc
    22261 ggaacgtctc gggcagttta gttctgccga aacgcagcgt attataaccc tgctcaagcg
    22321 ggctgggtta ccggtcaatg ggccgcgcga aatgtccgcg caggcgtatt taccgcatat
    22381 gctgcgtgac aagaaagtcc ttgcgggaga gatgcgctta attcttccgt tggcaattgg
    22441 taagagtgaa gttcgcagcg gcgtttcgca cgagcttgtt cttaacgcca ttgccgattg
    22501 tcaatcagcg taacaacaag aaaggtcagg ccgcttatca agcggtctat tagcttcagg
    22561 ttaattgcaa cgtggtaagc attaaccttt tagtggggtg ttaaatggat gaattcaaac
    22621 cagaagacga gctgaaaccc gatcccagcg atcgtcgtac tggtcgttct cgtcaatctt
    22681 ctgaacgttc tgagcgtact gaacgtggcg aaccgcagat caattttgat gatattgaac
    22741 ttgatgacac tgacgatcgc cgtccgactc gtgcgcaaaa agagcgcaat gaggaaccgg
    22801 aaatcgaaga agaaattgac gaatccgaag atgaaactgt ggatgaagag cgcgtagagc
    22861 gtcgtccgcg taagcgcaaa aaagcagcca gtaaacccgc ttctcgtcag tatatgatga
    22921 tgggtgtcgg cattctggtt ctactgctgt tgatcatcgg tatcggttct gcgctaaaag
    22981 ccccctcgac ctcttccagc gatcaaaccg cgtctggcga gaagagtatt gatcttgctg
    23041 gcaatgcgac cgatcaggcg aatggcgtgc agccagtgcc gggaaccacg tctgcggaaa
    23101 atactcagca ggatgtttct ctgccaccga tctcttctac gccgactcaa gggcaaaccc
    23161 cggtggcaac ggatggtcaa caacgtgttg aagtgcaggg tgacctgaac aatgcgctga
    23221 cccagccaca aaatcagcaa cagttgaaca atgtggcggt caattctaca ttgccgactg
    23281 aaccagcaac tgtcgcgcca gtacgcaatg gcaatgcatc gcgtgacacg gcgaaaacgc
    23341 aaactgctga acgtccggcc actacgcgtc cagctcgtca gcaggcggtg attgaaccga
    23401 aaaaaccgca agcaaccgtg aaaacggaac cgaagccggt agcacagacg ccgaagcgta
    23461 ctgaaccagc tgctcctgcg gcgagcacga aggcaccggc tgcgacttct acgccagcac
    23521 caaaagagac ggcggctacg gctccagtac agacggcatc cccggcgcaa accacggcaa
    23581 caccagccgc tggagggaag accgcaggta atgttggttc gttgaaatcg gcaccgtcca
    23641 gccattacac tctgcagctg agcagttcct ctaactacga caacctgaac ggttgggcga
    23701 agaaagagaa tctgaaaaac tacgttgtct atgaaacgac gcgtaatggt cagccgtggt
    23761 atgtcctggt ttctggcgtg tacgcttcga aagaagaggc gaaaaaagcg gtatctacat
    23821 tgccagcaga tgtccaggcc aaaaacccgt gggcgaaacc gctgcgtcag gtacaggccg
    23881 atctgaagta atcaaggtta tctcccgcaa tggtttatcg ttgcgggagt tgcctgaagc
    23941 gctggatgct gtcggagctt tctccacagc cggagaaggt gtaattagtt agtcagcatg
    24001 aagaaaaatc gcgctttttt gaagtgggca gggggcaagt atcccctgct tgatgatatt
    24061 aaacggcatt tgcccaaggg cgaatgtctg gttgagcctt ttgtaggtgc cgggtcggtg
    24121 tttctcaaca ccgacttttc tcgttatatc cttgccgata tcaatagcga cctgatcagt
    24181 ctctataaca ttgtgaagat gcgtactgat gagtacgtac aggccgcacg tgagctgttt
    24241 gttcccgaaa caaattgcgc cgaggtttac tatcagttcc gcgaagagtt caacaaaagc
    24301 caggatccgt tccgtcgggc ggtactgttt ttatatttga accgctacgg ttacaacggc
    24361 ctgtgtcgtt acaatctgcg cggtgagttt aacgtgccgt tcggccgcta caaaaaaccc
    24421 tatttcccgg aagcagagtt gtatcacttc gctgaaaaag cgcagaatgc ctttttctat
    24481 tgtgagtctt acgccgatag catggcgcgc gcagatgatg catccgtcgt ctattgcgat
    24541 ccgccttatg caccgctgtc tgcgaccgcc aactttacgg cgtatcacac aaacagtttt
    24601 acgcttgaac aacaagcgca tctggcggag atcgccgaag gtctggttga tcgccatatt
    24661 cctgtgctga tctccaatca cgatacgatg ttaacgcgtg agtggtatca gcgcgcaaaa
    24721 ttgcatgtcg tcaaagttcg acgcagtata agcagcaacg gcggcacacg taaaaaggtg
    24781 gacgaactgc tggctttgta caaaccagga gtcgtttcac ccgcgaaaaa ataattctca
    24841 aggagaagcg gatgaaacag tatttgattg ccccctcaat tctgtcggct gattttgccc
    24901 gcctgggtga agataccgca aaagccctgg cagctggcgc tgatgtcgtg cattttgacg
    24961 tcatggataa ccactatgtt cccaatctga cgattgggcc aatggtgctg aaatccttgc
    25021 gtaactatgg cattaccgcc cctatcgacg tacacctgat ggtgaaacct gtcgatcgca
    25081 ttgtgcctga tttcgctgcc gctggtgcca gcatcattac ctttcatcca gaagcctccg
    25141 agcatgttga ccgcacgctg caactgatta aagaaaatgg ctgtaaagcg ggtctggtat
    25201 ttaacccggc gacacctctg agctatctgg attacgtgat ggataagctg gatgtgatcc
    25261 tgctgatgtc cgttaatcct ggtttcggcg gtcagtcttt cattcctcaa acactggata
    25321 aactgcgcga agtacgtcgt cgtatcgacg agtctggctt tgacattcgt ctggaagtgg
    25381 acggtggcgt gaaggtgaac aacattggcg aaatcgccgc ggcgggcgcg gatatgttcg
    25441 ttgccggttc ggcaatcttc gaccagccag actacaaaaa agtcattgat gaaatgcgca
    25501 gtgaactggc aaaggtaagt catgagtaag tttgaagata ttcgcggcgt cgcttttgat
    25561 ctcgacggta cgctggtgga cagtgctccc ggtcttgctg ctgcggtaga tatggcgctg
    25621 tatgcgctgg agttgcccgt tgcaggtgaa gaacgtgtta ttacctggat tggtaacggc
    25681 gcagatgttc tgatggagcg cgcattgacc tgggcgcgtc aggaacgtgc gactctgcgt
    25741 aaaacaatgg gtaaaccgcc cgttgatgac gacattccgg cagaagaaca ggtacgtatt
    25801 ctgcgtaaac tgttcgatcg ctattatggc gaggttgccg aagaggggac gtttttgttc
    25861 ccgcacgttg ccgatacgtt gggcgcgttg caggctaaag gcctgccgct aggcctggtc
    25921 accaacaaac cgacgccgtt cgtcgcgccg ctgctcgaag ccttagatat cgccaaatac
    25981 ttcagcgtgg tgattggtgg tgatgatgtg caaaacaaaa aaccgcatcc ggacccgctg
    26041 ttactggtgg ctgagcggat gggaattgct ccacaacaga tgctgtttgt tggcgactca
    26101 cgcaatgata ttcaggcagc aaaagcggca ggttgcccat cagttggctt aacctacgga
    26161 tataactacg gcgaggctat cgatctcagc cagcctgatg taatttatca gtctataaat
    26221 gaccttctgc ccgcattagg gcttccgcat agcgaaaatc aggaatcgaa aaatgactaa
    26281 gcccatcgtt tttagtggcg cacagccctc aggtgaattg accattggta actacatggg
    26341 tgcgctgcgt cagtgggtaa acatgcagga tgactaccat tgcatttact gtatcgttga
    26401 ccaacacgcg atcaccgtgc gccaggatgc acagaagctg cgtaaagcga cgctggatac
    26461 gctggccttg tatctggctt gtggtatcga tcctgagaaa agcaccattt ttgttcagtc
    26521 ccacgtaccg gaacatgcgc agttaggctg ggcactgaac tgctatacct acttcggcga
    26581 gctgagccgc atgacgcagt ttaaagataa atctgcgcgt tatgccgaga acatcaacgc
    26641 tggtctgttt gactatccgg tgctgatggc tgcggacatc ctgctgtatc aaactaatct
    26701 ggtaccggtg ggtgaagacc agaaacagca tctcgaactg agccgcgata tcgcccagcg
    26761 tttcaacgcg ctgtatggcg atatctttaa ggtgccggag ccgtttattc cgaaatctgg
    26821 cgcgcgcgta atgtcgctgc tggagccgac caagaagatg tccaagtctg acgataaccg
    26881 caataacgtt attggcctgc tggaagatcc gaaatcggta gtgaagaaaa tcaaacgcgc
    26941 ggtcaccgac tccgacgagc cgccggtagt tcgctacgat gtgcagaaca aagcgggcgt
    27001 ttccaacctg ctggatatcc tttctgcggt aacgggccag agtatcccgg aactggaaaa
    27061 acagttcgaa ggcaagatgt atggtcatct gaaaggcgaa gtggctgatg ccgtttccgg
    27121 tatgctgact gaattgcagg aacgttacca ccgtttccgc aacgatgaag ccttcctgca
    27181 acaggtgatg aaagacggcg cagaaaaagc cagcgcgcac gcttcccgta cgctaaaagc
    27241 ggtgtacgaa gcgattggtt ttgtggcgaa gccgtaatat caaaacgctc ggacggtccc
    27301 ctcgcccctt tggggagagg gttagggtga ggggaacgtg cgggatgtaa aattcagtgc
    27361 gccggcatcg ccggatgcga tgctggtgca tcttatccgg cctacggtta atgtctttta
    27421 ttatcaatcg ttaaggtgat gcggttggcg cggcagaaga gttcggagac gtgcaccggt
    27481 ttgtcattct gatcaaaggc gattttgctg atgcgaaaca acggttcgcc cagttcgcat
    27541 ttcagccatt tcgcctgcgg gcgggtggcg gcaaagatat cgatggtctt tttgtcgctg
    27601 accactcgcg tgtcaaaacg ctcctgaaat aactgatagg tggagcttcc ttcgacgtaa
    27661 atctcatcaa agtcaggata acgcgacagc gggatccacg aactatcaat aaatagcggc
    27721 tctttatcga gatacatcac ccggcaaaga tggaacactt cgctgttgcc agggatgttc
    27781 agcttttcgc aaaacggcgc ggcgctgacc cgttcctgct cgatcacttt ctctttcgtc
    27841 gccttgcctt gtgagacgcc aaaatcggta aaaccactga cggtcagcag ggcgttttcg
    27901 accttctggc tttgtacaaa ggtgcctttg ccttgccagc ggatcagtac gccgtctgcc
    27961 actaagtcgc tgatagcttt gcgaatagta atgcggctga cgttatattg cgtacaaagc
    28021 tcgttttcgg tagggatctg ttgcccggcc aggtaaaccc cctgcgcgat atcatccagc
    28081 agccgctggc ggacggtagc gtagaggagt tgatgagagt agcggtccgt agctgacata
    28141 atcgaccctg aaataaagaa tgaattactc ggattatagg gtcgttttcc ggcaagtaac
    28201 agcacgttgg cgtgaagatg ctcacgccaa cgttatacct accaggcacc gtggtactga
    28261 atggttttcg ccgcgcacgc cgttccctgc gccatcgcct gcggtaatgt catccccgca
    28321 gaccagccgc aaaggaatcc ggcaatgaac gaatctccgg cacccatggt gtcgataacc
    28381 gtcaccagtt caggagcctg acgccagaac tgcgcgccat cccaggcaat gctgccgttt
    28441 tcgcccagcg tgacaatcac tgttcctgcg ccacgggcaa caatcgcttt catcttcaga
    28501 cgcagcgctt cgtcttcttg cggtgcagag gcaaaggcaa aatcgagatg cggcaccagt
    28561 gtctgccaga gcgggctgtc ccacttgtcg gagaagtcga aagcggtaag tttgcccgca
    28621 gcgtgcagct gtgggaatgc gtcttccgca tgtccccaga ttgccgcgtg cacaatgtca
    28681 tactgcgcca gccaggcgta atcctcttca ctcagggcaa agtcggccat cacgccttcg
    28741 gtgtagtcgc caaaaacgcg atcattgtcg tgcagttcca cctgagtttg tgcggtaacg
    28801 ccgtgtttcg tatggacatg gctgatatcg acgcccatgc gggcgagatc ctgcttaagc
    28861 tttgtgccgt agtcatcgtc acccacccag gtaatgcatc ccggctttat accgtagcga
    28921 gtgcagtaca ccgccacgtt caccgcatta ccgccagaaa acgctttatt cagttgcggg
    28981 tagatatcga cgcagttatc gccgattgtc gccagggttt tcatcactca tcctccggca
    29041 gcagcgcgcg aaagcgttcc agcgcctggc gggcgtaaag tctgggctcg ttcatataca
    29101 tcgttaccag ttccactgtg cagtatccct catagccccg atcaataata tcgcgcatca
    29161 gttcccgcag cggcattttg ccttcgccgg gaatgtaatg cgtgtcgctg gccccgtcgc
    29221 tgtcgacaat atgcagatga cgtaatttat cgcccagttt gtcgaaataa ctcatcaccg
    29281 gttccgcctg gacatacggc gcgcaaatgt cgaccatgct gaacaggcgc ggcgaaggca
    29341 ccagcgccag cgcatgaagc acatcattag cattacacac gacgttcgat tcatacggcg
    29401 ttaacggttc gaggatcaga tccatgccga tgttttccgc gtactcacac agttcgctta
    29461 agttctctgc cagccgcccc cagataacgt taggcggcgt gagatagccc gcgtgggccg
    29521 cagaaatcag cgtataaccc gcgttcatct cttttgccat atccatcgcc agcttgatca
    29581 tgtccaggct ttcacgacgc atatgttcat cgcccagcat catgttatac ggatagccgt
    29641 tggtttctgg cgtgtaaccg ataatcggca tctgatacgt ctgcgccagc gccttgattt
    29701 gtttgatgcc gcccgctttt aagtccggcg cgaacgcgtg cgggcgaccg ccccaaattt
    29761 cgatgccgtc gtaacccagc tcgcttgcat cacggaatgc gtgttcaata ggcagccgct
    29821 ggtggccgca ggtaaacata cctgttttca tcgggtaact ccttgcgtaa atccggcccg
    29881 ttgccgggcc gggagaggga ttaatattcc accagaccac cgtaatagcg gcgttcatcc
    29941 gggttgtgat ctttgtaaat agacaggtag tagcagagcc actccattgg cacgaacatc
    30001 aggaacggtg ccagccacgg gtgcagccct tgcgaaattt cggcgtaatc gatgacgatc
    30061 acgttgtcag tacgctgttt aacaaagtta atggcgcgtt cggtggtgtg acgactttcg
    30121 tcattgccga gcagaaacag gaacggaacg cccggttcga caatctccag cgggccgtga
    30181 cggaactcac cgctctcaat cacacagccg tgcgtccagg taaattccat cagcgtcacg
    30241 atgccttctt tgtaacccag cgggcgcagc gggcctgcgg caaccgtgta aatcatcggc
    30301 cactgactgg cctgttcacc gagctggcga cctttctctt cccaagtgcg taccagatga
    30361 ccgagcgcat ttggcagctg cttgagatcg tttttgatct tgccgatttc cgcgtgcggc
    30421 gcgaggcggg tgatcatctc cagcaccacg ctgtagcaga gcagcagatg aatttcccag
    30481 atacagtcgg cctgataatc aatgctaaat tccgccgccg aggtaatcgg gctatccgcg
    30541 cgtttggtga atgccgcagt aagcgcgccg caggcccgac ccagttccag cgctttgatc
    30601 acctcttcgg ttttaccgta gtcagaaacg ccaatcaccg cgcaacgatc gtcgaggcga
    30661 tacggggtgt tatcgcagaa ctcccagccg gaaatggcgt aaacctgaag atcggaaaag
    30721 cgatctgcca gatgtttcgc cgtttgcgcc gcgttgagtg gcgatccgca ggcaacgaaa
    30781 taaatacgat cgatgtcgcg cttcaccatc tcttccacga tggtgtgcac cagcggaacg
    30841 tcatggctga gaactttttc cacttcctga accatatttt cggtgaccag aaagtccacg
    30901 gtgcttttat caatatccaa cattttattt tctccagata acattacgac aaagcgttga
    30961 gctggcggct acgcttcgcc cagaaggcgt aagcaggcag accagtggca ataacgatga
    31021 cggcgcagat aaggccggga atcggtgccc agacaaaggt tgaggcgacc agaatgaggc
    31081 ttgatgcaat ggcgagggtg gtcatcagcc cgaaagacgg agtacgccac agcggtttgt
    31141 aatcgtcgcg tttacgacac cagatgatgg agccgaaggt gagggtattt ttgaaacaca
    31201 tcaccagggt gaaataaccc agcaggctgg tgagatcgga aacgaagatg aagaagatcc
    31261 ccagcgcccc ttgcaggatg atggagacat ccggcgtgtt gtattttgga tgcacatggc
    31321 cgaagcattt gaagaacaga ttgtctttcg ccatcgcgta ttccagccgc ggctggtaca
    31381 tcacgcagct ggaaagcgat ccgagaatga cgatcatcgc cgtgatagcc acgaagatcc
    31441 cggcggtgct gccgagtgcc gggatccagg tcagggcgtc ggaaatcggc gtttcagaat
    31501 tggcgagttt gtcgaagggc atcaggccag aaattaccaa cgccagcagg gtgtagagca
    31561 ccagaaccag cagacaggaa ccaattagcg ctcgtggcat agttttaccg gggtttttaa
    31621 tttcgccggt catataacag atagaggcca tgccggtgta cgaccaactg gtggcggaga
    31681 tccccgccag tagcgccata aagctgcccg ttgcgccaat cgcagtggtg gtaggggcgg
    31741 caaaattctc cgctttaaac cagaagatcc caagaccaat gacgatagtg aacgggataa
    31801 ttttggcgat ggtaattagc gtctgaaacg ctgcgccgcc ttcaacggag cgcaggtgca
    31861 gcagcataaa ggcgataatt aatccggcgg cgataaattt accgagcaga ggatcgatag
    31921 gcgttaaaaa gccaagattg ctgactatcg ccagcgccat aatcgacaat gacggcgcat
    31981 cgttggccca gaagctggcc cagccggaga ggaaagccag cggtcggctt ccggcatttt
    32041 tcagataaac ataatctgcg ccattttccg gataagcggt ggatagttcc gcatagacgc
    32101 acatttgcgg gatcacaatt aacccgccga tgacaaatgc gagcaccgtc agccacggcg
    32161 tgcccgccgc ttttgccact tcacccacag atacaaaaat accggagccg acggttgtcc
    32221 cgacggcgat tgcaagaacg gcccaaaatc cgagcttgcg ttggagttcc tggcttccca
    32281 tagcgatacc tttttcttgt ccaacacgtt gtaggggaga gtttgcagtc atttaaaagg
    32341 taacatgaca atgcatgatg aatataacaa catatgatgt tatgtaatgt ggaggaagat
    32401 cacataattt cgcacaggat cgcgctgtgg ctaatggatg tagttatcaa attgaaatta
    32461 aagtgaaaat atttttacgg gtggggagca agaaggatat agaaacaaat acgccctcgg
    32521 aaaatccaga gggcgtcggg caattaaacc ggtgttagcc gatttctgtc agagacttac
    32581 tgtgcagtag gaccgcaacc gccaatgatt ttagaaatag aaattgctgg gtgcagcagg
    32641 tagtcgtaag agcagttctt atcacggttt tcaatatgac cagtacatgc agttaacgta
    32701 gccagtactg cacctaccag tgcaacttta ataaa
//
LOCUS       DAZUCE010000026        32643 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZUCE010000026 DAZUCE010000000
VERSION     DAZUCE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.026"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(133..3282)
                     /gene="acrB"
                     /locus_tag="ACHY03_001103"
     CDS             complement(133..3282)
                     /gene="acrB"
                     /locus_tag="ACHY03_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFS3904363.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3305..4498)
                     /gene="acrA"
                     /locus_tag="ACHY03_001104"
     CDS             complement(3305..4498)
                     /gene="acrA"
                     /locus_tag="ACHY03_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFS3904364.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4640..5287
                     /gene="acrR"
                     /locus_tag="ACHY03_001105"
     CDS             4640..5287
                     /gene="acrR"
                     /locus_tag="ACHY03_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFS3904365.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5415..8777
                     /gene="mscK"
                     /locus_tag="ACHY03_001106"
     CDS             5415..8777
                     /gene="mscK"
                     /locus_tag="ACHY03_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFS3904366.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8838..8936
                     /locus_tag="ACHY03_001107"
                     /pseudo
     CDS             8838..8936
                     /locus_tag="ACHY03_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8989..9150)
                     /gene="rsmS"
                     /locus_tag="ACHY03_001108"
     CDS             complement(8989..9150)
                     /gene="rsmS"
                     /locus_tag="ACHY03_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFS3904367.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9164..9691)
                     /gene="priC"
                     /locus_tag="ACHY03_001109"
     CDS             complement(9164..9691)
                     /gene="priC"
                     /locus_tag="ACHY03_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFS3904368.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9761..10138
                     /gene="ybaN"
                     /locus_tag="ACHY03_001110"
     CDS             9761..10138
                     /gene="ybaN"
                     /locus_tag="ACHY03_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFS3904369.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10291..10842
                     /gene="apt"
                     /locus_tag="ACHY03_001111"
     CDS             10291..10842
                     /gene="apt"
                     /locus_tag="ACHY03_001111"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFS3904370.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10971..12902
                     /gene="dnaX"
                     /locus_tag="ACHY03_001112"
     CDS             10971..12902
                     /gene="dnaX"
                     /locus_tag="ACHY03_001112"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFS3904371.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12235..12299
                     /gene="dnaX"
                     /locus_tag="ACHY03_001112"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12955..13284
                     /gene="ybaB"
                     /locus_tag="ACHY03_001113"
     CDS             12955..13284
                     /gene="ybaB"
                     /locus_tag="ACHY03_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFS3904372.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13284..13889
                     /gene="recR"
                     /locus_tag="ACHY03_001114"
     CDS             13284..13889
                     /gene="recR"
                     /locus_tag="ACHY03_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFS3904373.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13999..15873
                     /gene="htpG"
                     /locus_tag="ACHY03_001115"
     CDS             13999..15873
                     /gene="htpG"
                     /locus_tag="ACHY03_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFS3904374.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16054..16698
                     /gene="adk"
                     /locus_tag="ACHY03_001116"
     CDS             16054..16698
                     /gene="adk"
                     /locus_tag="ACHY03_001116"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFS3904375.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16830..17792
                     /gene="hemH"
                     /locus_tag="ACHY03_001117"
     CDS             16830..17792
                     /gene="hemH"
                     /locus_tag="ACHY03_001117"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFS3904376.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17789..18748)
                     /gene="aes"
                     /locus_tag="ACHY03_001118"
     CDS             complement(17789..18748)
                     /gene="aes"
                     /locus_tag="ACHY03_001118"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFS3904377.1"
                     /translation="MKPENKLPVLELISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18900..20204
                     /gene="gsk"
                     /locus_tag="ACHY03_001119"
     CDS             18900..20204
                     /gene="gsk"
                     /locus_tag="ACHY03_001119"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFS3904378.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20338..22014)
                     /gene="ybaL"
                     /locus_tag="ACHY03_001120"
     CDS             complement(20338..22014)
                     /gene="ybaL"
                     /locus_tag="ACHY03_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFS3904379.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22252..23472)
                     /gene="fsr"
                     /locus_tag="ACHY03_001121"
     CDS             complement(22252..23472)
                     /gene="fsr"
                     /locus_tag="ACHY03_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFS3904380.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23690..25342
                     /gene="ushA"
                     /locus_tag="ACHY03_001122"
     CDS             23690..25342
                     /gene="ushA"
                     /locus_tag="ACHY03_001122"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFS3904381.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNLGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25379..25858)
                     /gene="ybaK"
                     /locus_tag="ACHY03_001123"
     CDS             complement(25379..25858)
                     /gene="ybaK"
                     /locus_tag="ACHY03_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFS3904382.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26062..26856)
                     /gene="ybaP"
                     /locus_tag="ACHY03_001124"
     CDS             complement(26062..26856)
                     /gene="ybaP"
                     /locus_tag="ACHY03_001124"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFS3904383.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPARLLKKLKNADALIVEADVSNNDTPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26994..27335
                     /gene="ybaQ"
                     /locus_tag="ACHY03_001125"
     CDS             26994..27335
                     /gene="ybaQ"
                     /locus_tag="ACHY03_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFS3904384.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSISAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27550..30054)
                     /gene="copA"
                     /locus_tag="ACHY03_001126"
     CDS             complement(27550..30054)
                     /gene="copA"
                     /locus_tag="ACHY03_001126"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFS3904385.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     NVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFVDFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPITANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30316..31248
                     /gene="glsA"
                     /locus_tag="ACHY03_001127"
     CDS             30316..31248
                     /gene="glsA"
                     /locus_tag="ACHY03_001127"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFS3904386.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31251..32543
                     /gene="ybaT"
                     /locus_tag="ACHY03_001128"
     CDS             31251..32543
                     /gene="ybaT"
                     /locus_tag="ACHY03_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFS3904387.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         7905 a         8639 c         8579 g         7520 t
ORIGIN      
        1 ttttatgaat ttatccgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc
       61 aattactcct taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttggtga
      121 ttacacgttg tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg
      181 gctaaagcgg cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt
      241 tgcggtcacc atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc
      301 accagtactg ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat
      361 cggacgtaaa cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt
      421 atccatcaag tctttggcga attcgacgat aaggatcgcg ttcttcgccg acaacccaat
      481 ggttgtgagc aggcctacct ggaagtaaac gtcattggtc aggccacgga aggttgcagc
      541 cagcaacgca ccgataaccc ccagcggaac gaccagcata acggagaacg gaatcgacca
      601 gctctcgtac agcgccgcca gacacaggaa cacgacaatc aacgaaatcg cgtacagtga
      661 aggtgcctgg ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac
      721 accggtaggc agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt
      781 acccggtgcc gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg
      841 cggcgaaccg tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc
      901 agcagcacga acataccagt cgccgatatc atccggcagc atacggtatt ttgcttctga
      961 catgacgtaa actttcttca cacgaccgcg gtcgataaag tcgttcacat agctgccgcc
     1021 ccatgcagcg cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt
     1081 ttcctggtcg atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct
     1141 ggtcaacata tcagggtgct tcgctgcttc tgcaagcaac tggttacgcg cctgagtcag
     1201 tttttcgtga ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcggtacc
     1261 cagttccacg attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa
     1321 agcgcgtgtt gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc
     1381 ccagtccttc aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa
     1441 gccgttaacg gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt
     1501 tacctcattg agcactttct gtgtacgttc ctgcgttgca cctgctggca gctgaaccat
     1561 ggtcataaac acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag
     1621 ataggccatg ccgaccacga tgatcagata cagcaccagg taacgccccg tactgcgcag
     1681 aataccgcct acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca
     1741 gccgaagaag ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt
     1801 ggcacaaaga gctggagtca ggatcaacgc caccagtacc gacagcgcca ttgctgaaac
     1861 aatggtaata gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg
     1921 tacgaatacc gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat
     1981 cgacttacgg gtggcttctt ttggcggcaa accttcttcc gccatcacac gctcaacgtt
     2041 ttctaccaca acgatagcgt catccaccaa caggccgatg gcgagcacca tcccgaacat
     2101 tgttagcgtg tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac
     2161 caccggtacg gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat
     2221 aaccaggaac acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat
     2281 tttcacgaac ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg
     2341 ttccatcttc gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt
     2401 cgccagcttg atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta
     2461 gttctcacca cccagctcaa tcttcgccac atcacgcagc agcacgcggg aaccatcctg
     2521 attcactttc agcaggattt tgccaaactc ttcagtagag gtcagacgcg tctgagcaat
     2581 aatagaggcg ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc
     2641 aacctgggcg ttctgcgctt tgatggcggt aataacatca accggtgtta gctggaattt
     2701 gttcagttca ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac
     2761 gtcacccacg ccagacgtac ggctgatggc atctttcata ttcgccgcca cgtagtcgga
     2821 gatatcctcc tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct
     2881 gctggatgat ttctcaacgc tcacaccttg ctgctgaact tcttgcggca gcaacggcat
     2941 cgccagctgc agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc
     3001 aaaggtcagg gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag
     3061 gttatcgata ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt
     3121 cgcatcagcg ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata
     3181 ttgcgccacc ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat
     3241 cacccacgca aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt
     3301 taagttaaga cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag
     3361 cggtaacttc ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta
     3421 ctacgcgatc gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc
     3481 ttgcaacgat cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat
     3541 cgccacgcgg cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc
     3601 cttcttccag acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga
     3661 agatagcgcg tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca
     3721 gcgtaccgtc ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc
     3781 cgttctcttg tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc
     3841 tggactgggt cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg
     3901 cctgaccgtt ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa
     3961 tcggagaggt gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg
     4021 ccgcagttac cgcagcattc gcctgttgcg catcagccag agcctgatcg tactcttgct
     4081 tactgatgta ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat
     4141 tggctgcagc ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg
     4201 tcgcaggatc aatctgatag agagagacac ctgcttcgat gtcgctacct tctttgaaat
     4261 tacgcttcag gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg
     4321 tgcgacccgg aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa
     4381 cggcgggcat ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta
     4441 agctgcctga gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat
     4501 gtaaacctcg agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc
     4561 tgcgtttata ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg
     4621 cctgtaaatt cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca
     4681 acacatcctc gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct
     4741 gggcgagatt gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga
     4801 caagtcggat ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga
     4861 gcttgagtat caggcaaaat tccctggcga tccactctca gtattaagag agatattaat
     4921 tcatgttctt gaatccacgg tgacagaaga acggcgtcga ttattgatgg agattatatt
     4981 ccacaaatgc gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg
     5041 tctggaaagt tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt
     5101 gcctgcggat ttaatgacgc gtcgcgcagc aattattatg cgcggctata tttccggcct
     5161 gatggaaaac tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta
     5221 cgttgccatc ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa
     5281 cgaataaccc tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg
     5341 gttcactgcg tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga
     5401 aaccttcatt catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt
     5461 cagcatttat tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt
     5521 catcgaatgg tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata
     5581 aacaaaaaga tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag
     5641 ccaccctcga taaaatcgat cgcgtaaaag aagagaccgt tcagctacgg caaaaagtcg
     5701 ctgaagcgcc ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg
     5761 ataacgacga agaaacgcgc aaaattctga gcacgctttc gttgcgccag ctggaaactc
     5821 gcgttgccca ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca
     5881 gccagctggt ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt
     5941 cgcagcagct gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct
     6001 tacgtcccag ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg
     6061 accagcagcg taaaagcctg gaagggaata ccgtcttgca ggataccttg caaaagcaac
     6121 gtgattacgt gacggcgaac agcgcccgtc tggaacacca gttgcagctg ttgcaagaag
     6181 cggtaaacag caagcgcctg actttaaccg aaaaaacggc gcaggaagcc gtctccccgg
     6241 atgaagccgc gcgtattcag gctaatccgc tggtgaagca ggaactggaa attaaccagc
     6301 agttaagtca gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca
     6361 ttaaagtcaa aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga
     6421 ttgccgtcct gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc
     6481 tgccctcggc ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac
     6541 agtttgaagt taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac
     6601 tggaagaagg tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg
     6661 ttgatatgcg tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga
     6721 tggccattaa cctgcaaatc aaccagcagc agttaatgag tgtgtcgaaa aacctgaaat
     6781 ccatcctgac tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga
     6841 tcaaagcgtt cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatt acggttaact
     6901 gggaaaaagc ctggcccgcc gtttttatcg ctttcctcgc tggtttgccg ctgctgttga
     6961 ttgccgggct gatccactgg cgtctggggt ggctgaaagc gtatcaacaa aaactggctt
     7021 ccgctgtggg ttccctgcgt aacgacagcc agctcaacac accaaaagcg atccttatcg
     7081 acctgatccg tgcactgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga
     7141 ccatgcagct caacatcagc gaactgctat ggtcgttcag caaaaaactg gcgattttct
     7201 ggctggtgtt tggcctgtgc tggaaagtgc tggagaagaa cggcgttgcc gtgcgtcact
     7261 tcggcatgcc ggaacagcag accagccact ggcgtcggca aattgtccgc atcagtctcg
     7321 cgttgctgcc tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg
     7381 atgatgtgct ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat
     7441 ggccaatgtg ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctggtcacca
     7501 ttaccgtgct gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct
     7561 acaccacgct gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga
     7621 acttgctcta ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc
     7681 gtcgtgcgct ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc
     7741 cggaagaacc caccattgca ctggagcagg ttaaccagca gacgctgcgt attaccatgt
     7801 tgctgatgtt tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg
     7861 tgttcagcta tctcgacagc attacgctgt ggcattacaa cggtactgaa gctggtgctg
     7921 cggtggtgaa aaacgtcacc atgggtagtc tgttgtttgc gattatcgcc tcaatggtgg
     7981 cctgggcgtt gattcgcaac ctgcctggtt tgctggaagt gctggtgctc tcgcgactga
     8041 atatgcgcca gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgctg
     8101 ttggtgcgat gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg
     8161 cagcagcatt atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct
     8221 ccggtttgat cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta
     8281 gcttctcggg gacagtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc
     8341 gcaaagaagt gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt
     8401 tgactgacac tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg
     8461 aaaaagtgcg taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac
     8521 caatgccgga agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc
     8581 tgtatgtgcg tgaactgcgt gaccgtagtc gtactgtcga tgagctgaac cgtactatcg
     8641 atcagctgtg ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc
     8701 acaacgagaa gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga
     8761 cgccagcggt agggtaataa cgaaaggggg cgacattttg ttgccccgag attgctaaca
     8821 aagtgcgcgt tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca
     8881 aaaattcaat atattgcagg atcggtgtag gcctgataag cgtagcgcat caggcagttt
     8941 tgcgtttgcc cgcaacctta ggggacattt agccacccca tttatttctc acttttccgc
     9001 ctcatcatcg cgcgttaatt tcttttcata atcgcgtttt acaatatcca gcgcgcgcag
     9061 cacggtgcgg gctgggatct gattttcttc cagcagcaca attaaatcga cggccagttt
     9121 gacatcgtcg ggggcatttt ccagtgacat actctctcca ttgctagcgg gttaaacgcg
     9181 ctaacctgtt ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg
     9241 cttccacttc acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg
     9301 ctctgcgttc ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt
     9361 tacgctgcca gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg
     9421 aggcttcacg cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt
     9481 gcggcagctg ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt
     9541 cgagacaagc ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac
     9601 ttagcgtggc gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt
     9661 ccagtttttc cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt
     9721 tatgctaaag tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat
     9781 catcattggc tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc
     9841 gacgacgccg tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca
     9901 cgcctggttg ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca
     9961 tgcgatgccg cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat
    10021 ttctctgtgg ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg
    10081 tttgcttttc tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag
    10141 cacaacaatc gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac
    10201 aggcgcaggc ggtcaaaggt taaacaactg ttacttttga tacgtttaaa acgcgccgtg
    10261 agtaccaccg taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat
    10321 ctcaaaaata gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat
    10381 gtcaccagct tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag
    10441 cgttacaaaa atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt
    10501 ggcgctccgg tagctctggg gttgggcgtt ggctttgtac cggtccgtaa accgggcaaa
    10561 ctgccgcgtg aaactatcag tgaaacttac gatctggaat acggcaccga tcagctggag
    10621 atccacgttg atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca
    10681 accggcggca ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct
    10741 gacgctgcgt tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag
    10801 ggcattacca gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc
    10861 tgcccacgct acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg
    10921 ttagcattac cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc
    10981 aggtcttagc ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg
    11041 tgctgaccgc actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt
    11101 ccggtacccg tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact
    11161 gcgaaaccgg cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc
    11221 aggggcgctt tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata
    11281 cccgcgacct gctggataac gtccagtacg ctccggcgcg cggtcgtttc aaagtttatc
    11341 tcatcgacga agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg
    11401 aagagccgcc ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg
    11461 tgacgatttt gtcgcgctgt ttgcaatttc atctcaaggc gctggatgtc gagcaaattc
    11521 gtcatcaact tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc
    11581 aattgctggc gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg
    11641 cgattgccag cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc
    11701 ttgacgacga tcaggcgctg tcgctggttg aagcgatggt cgaggccaac ggcgagcgcg
    11761 taatggcgct gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg
    11821 aaatgctcgg cctgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca
    11881 acgacatggc cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg
    11941 atattcagct ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg
    12001 accgccgcat gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc
    12061 cgctgcctga gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa
    12121 tgacgccaac ccaggtgccg ccgcaaccgc aatcagcgcc gcagcaggca ccgactgtac
    12181 cgctcccgga aaccaccagc caggtgctgg cggcgcgcca gcagttgcag cgcgtgcagg
    12241 gagcaaccaa agcaaaaaag agtgaaccgg cagccgctac ccgcgcgcgg ccggtgaata
    12301 acgctgcgct ggaaagactg gcttcggtca cagatcgcgt tcaggcgcgt ccggtgccat
    12361 cggcgctgga aaaagcgcca gctaaaaaag aagcgtatcg ctggaaggcg accactccag
    12421 tgatgcagca aaaagaagtg gtcgccacac cgaaggcgct gaaaaaagcg ctggaacatg
    12481 aaaaaacgcc ggaactggcg gcgaagctgg cggcagaagc cattgagcgc gacgcgtggg
    12541 cggcacaggt gagccaactt tcgctaccaa aactggtcga acaggtggcg ttaaatgcct
    12601 ggaaagagga gagcgacaac gcagtatgtc tgcatttgcg ctcctctcag cggcatttga
    12661 acaaccgcgg tgcacagcaa aaactggctg aagcgttgag catgttaaaa ggttcaacgg
    12721 ttgaactgac tatcgttgaa gatgataatc ccgcggtgcg tacgccgctg gagtggcgtc
    12781 aggcgatata cgaagaaaaa cttgcgcagg cgcgcgagtc cattattgcg gataataata
    12841 ttcagaccct gcgtcgattc ttcgatgcgg agctggatga agaaagtatc cgccccattt
    12901 gatcgtaagc acagcttacg ttcgtcatcc ttaacgtgat tgagagagaa acctatgttt
    12961 ggtaaaggcg gtctgggtaa cctgatgaag caagcccagc agatgcaaga aaaaatgcag
    13021 aaaatgcagg aagagatcgc gcagctggaa gtcaccggcg aatctggcgc aggtctggta
    13081 aaagtgacca tcaacggtgc acacaactgc cgtcgcgtag agatcgaccc gagcctgctg
    13141 gaagacgaca aagagatgct ggaagacctg gtggctgcag cattcaacga cgcagcacgt
    13201 cgtattgaag aaacgcagaa agaaaaaatg gcctctgtat cctccggaat gcagctgccg
    13261 cctggcttta agatgccgtt ctgatgcaaa ccagcccgct gttaacacag cttatggaag
    13321 cactgcgctg tctgccgggc gttggcccga agtcggcgca gcgtatggcg ttcacgctgc
    13381 ttcagcgcga tcgtagcggc gggatgcgtc tggcgcaggc gctcacccgg gcgatgtcgg
    13441 aaatcggcca ctgcgccgat tgccgcacct tcaccgaaca ggaagtctgt aacatctgtt
    13501 cgaatccgcg tcgtcaggaa aacggtcaaa tctgcgtggt ggagagtccg gcggacattt
    13561 acgccattga gcagacgggg cagttttcag gtcgttattt tgtgttgatg ggacatctgt
    13621 caccgctgga cggcatcggt ccggatgata tcggccttga tcgtctggaa cagcgtctgg
    13681 cagaggaaaa aatcactgaa gtgatcctcg ccaccaaccc tacggttgaa ggtgaagcta
    13741 ccgctaacta cattgccgag ctttgcgcgc aatatgacgt ggaagccagc cgaatcgctc
    13801 atggcgtacc ggttggcggc gagctggaaa tggtcgatgg caccacgctg tcacactccc
    13861 ttgccgggcg tcataagatt cgtttttaag caaacgagag caggatcacc tgctctcgct
    13921 tgaaattatt ctcccttgtc cccatctctc cctcatcctg tttttaacct taaaatggca
    13981 ttattgaggt agacctacat gaaaggacaa gaaactcgtg gttttcagtc agaagtgaaa
    14041 cagcttctgc acctgatgat ccattctctc tattccaata aagaaatctt cctgcgtgag
    14101 cttatctcta acgcctccga tgcggcggac aagttgcgtt tccgtgcgct ctctaacccg
    14161 gacctgtacg aaggtgatgg cgaactgcgc gttcgtgtct ctttcgataa agacaagcgc
    14221 acgctgacca tctccgataa cggcgtgggg atgacccgcg acgaagtgat tgaccatctg
    14281 gggactatcg ctaaatccgg taccaaatca ttcctcgaat ccctgggttc tgaccaggcg
    14341 aaagacagcc agctgatcgg tcagtttggt gttggtttct actctgcgtt tatcgtggcc
    14401 gacaaagtga ccgtgcgtac tcgcgcggca ggcgaaaaac cagaaaacgg cgtcttctgg
    14461 gaatcggctg gcgaaggtga atacaccgtt gccgacatca ccaaagaaga tcgtggtact
    14521 gaaatcaccc tgcatctgcg tgaaggcgaa gacgagttcc tcgatgactg gcgcgtgcgt
    14581 tccatcatca gcaaatactc cgaccatatc gcgctgccgg tagagatcga aaaacgcgaa
    14641 gagaaagacg gcgaaaccgt tatctcctgg gagaaaatta acaaagcgca ggcgctgtgg
    14701 actcgtaaca agtcggaaat caccgatgaa gagtacaaag agttctacaa acacatcgcc
    14761 cacgacttta acgatccgct gacctggagc cacaaccgtg ttgaaggtaa gcaggagtac
    14821 accagcctgc tgtacattcc gtcccaggct ccgtgggata tgtggaaccg cgatcataaa
    14881 cacggcctga aactgtatgt tcagcgtgtg ttcatcatgg acgacgcaga acagttcatg
    14941 ccgaactatc tgcgcttcgt gcgtggtctg attgactcca gcgatctgcc gctgaacgtt
    15001 tcccgtgaaa tcctccagga cagcacggta acgcgtaacc tgcgcaatgc gctgaccaag
    15061 cgtgtgctgc aaatgctgga aaaactggcg aaagacgacg cggaaaaata ccagaccttc
    15121 tggcaacagt ttggcctggt actgaaagaa ggtccggcgg aagatttcgc taaccaggaa
    15181 gcgatcgcca aactgctgcg ttttgcttct actcataccg attcttctgc gcagaccgtg
    15241 tctctggaag actacgtttc ccgcatgaaa gaagggcagg agaaaatcta ctacatcact
    15301 gcagacagct atgcggcagc gaagagcagc ccgcacctgg aactgctgcg taagaaaggc
    15361 atcgaagttc tgctgctttc cgaccgcatc gatgagtgga tgatgaacta tctgactgag
    15421 ttcgacggta aaccgttcca gtcggtgtct aaagttgacg agtcgctgga aaaactggct
    15481 gacgaagttg atgagagcgc gaaagaagcg gagaaagcac tgactccgtt catcgaccgt
    15541 gtgaaagccc tgctcggcga gcgcgtgaaa gatgtccgtc tgactcaccg tctgaccgat
    15601 acgccagcga tcgtttcgac cgacgcggac gaaatgagca ctcagatggc gaaactgttc
    15661 gctgcggcgg gccagaaagt gccagaagtg aaatacatct tcgaactgaa cccggatcac
    15721 gtactggtga aacgtgcggc agatactgaa gatgaagcga agttcagcga gtgggtagaa
    15781 ctgctgctgg atcaggcact gctggcagaa cgcggcacgc tggaagatcc gaacctgttt
    15841 attcgtcgta tgaaccagct gctggtttcc tgatgtaatg ccggatgacc ttcgtgtcat
    15901 ccggcatttt tcttttcatt atctgcactt tccgcaaatt atctcgccat taaccgtttc
    15961 agcccctggt gcctttcttg aggcaatcgc ctgatggtgg tatcgtttat cgctttttca
    16021 aaaaattcga cacattttaa ggggattttc gcaatgcgta tcattctgct tggcgctccg
    16081 ggcgcgggga aagggactca ggctcagttc atcatggaga aatatggtat tccgcaaatc
    16141 tccactggcg atatgctgcg tgctgcggtc aaatctggct ccgagctggg taaacaagca
    16201 aaagacatta tggatgctgg caaactggtc accgacgaac tggtgatcgc gctggttaaa
    16261 gagcgcattg ctcaggaaga ctgccgtaat ggtttcctgt tggacggctt cccgcgtacc
    16321 attccgcagg cagacgcgat gaaagaagcg ggcatcaatg ttgattacgt tctggaattc
    16381 gacgtaccgg acgaactgat cgttgaccgt atcgtcggtc gccgcgttca cgcgccgtct
    16441 ggtcgtgttt atcacgttaa attcaatccg ccgaaagtag aaggtaaaga cgacgttacc
    16501 ggtgaagaac tgactacccg taaagacgat caggaagaga ccgtacgtaa acgtctggtt
    16561 gaataccatc agatgacagc accgctgatc ggctactact ccaaagaagc ggaagcgggt
    16621 aataccaaat acgcgaaagt tgacggcacc aagccggttg ctgaagttcg cgctgatctg
    16681 gaaaaaatcc tcggctaatc aagccgcagc agatctgctt tcgggcaggt ctgctcatcc
    16741 acctcaggca atacctctca cagcaattag ttcttcttcc tcacttttcc gctacaatta
    16801 tcaacaagtt gaatcgataa gaggcggtaa tgcgtcagac taaaaccggt atcctgctgg
    16861 caaacctggg tacgcccgat gcccccacac ctgaagcggt aaaacgctat ctgaaacaat
    16921 ttttaagcga cagacgcgtg gttgatacct cacggttgtt atggtggcca ttgctgcgcg
    16981 gcgtgatttt gccgctgcgc tcgccgcgtg tggcgaagct gtatgcctct gtctggatgg
    17041 aaggtggctc gccgctgatg gtttacagcc gccagcaaca gcaggcgctg gcacaacgtt
    17101 taccggagac gcccgtagcg ctgggaatga gctacggctc gccatcactg gaaagcgccg
    17161 tagatgaact actggcagag catgtagatc atattgtggt gctgccgctt tatccgcaat
    17221 tctcctgttc tacggtcggt gcggtatggg atgaactggc acgcattctg gcgcgcaaac
    17281 gtagcattcc ggggatatcg tttatacgtg attacgccga taaccacgat tacattaatg
    17341 cactggcgaa cagcgtacgc gcttcttttg ccaaacatgg cgaaccggat ctgctgctgc
    17401 tctcttatca tggcattccc cagcgttatg cagatgaagg cgatgattac ccgcaacgtt
    17461 gccgcacaac gactcgcgaa ctggcttccg cactggggat ggcaccggaa aaagtgatga
    17521 tgacctttca gtcgcgcttt ggccgggaac cctggctgat gccttatacc gacgaaacgc
    17581 tgaaaatgct cggagaaaaa ggcgtaggtc atatacaggt gatgtgcccg ggctttgctg
    17641 cggattgtct ggagacgctg gaagagattg ccgagcaaaa ccgtgaggtc ttcctcggtg
    17701 ccggcgggaa aaaatatgaa tatattccgg cgcttaatgc cacgccggaa catatcgaaa
    17761 tgatggctaa tcttgttgcc gcgtatcgct aaagctgagc ggtaaagaac tgagcgccgt
    17821 cgcgaagagc ctcgtcggcg gttttcatca tccgtgaata atgcaaaaag gcgtgcagcg
    17881 tgcctgggta gagtttgaac tcacagggct gctgatgcgc cgctaacgtc tggtaaagca
    17941 gacggctgtc atccagcagc ggatcgaact ccgccccggc aataaaacag ggcggaactt
    18001 cgcgagtgag atcattatta aacagacagt aatacggcga ctcgcggtcc gcgtcgttgc
    18061 ttaaatacgc ctcttcatac atctgcaaat cctgttgcgt taagccatcc cagacaccgc
    18121 ccaacagacg acgagtcacg gaatcccgta atccgtaaag cccataccac agcaaaacgc
    18181 ccgcaacttt accgcaatcg atctgtttat cacgcaacca caacgcactg gcgagcgcca
    18241 gcatggcacc tgcggaatca ccggcaaagc caatgcggga catattgatt tgataatcct
    18301 ccgcctgctg gtggaaataa caacaagcag ccacaatttc ctctatcgct tgcggaaaac
    18361 gcgcttcagg tgaaagggtg taatcaatac caatcaccgt acattggctg tagcttgcca
    18421 gcaggcgcat gatgcgatcg tgggtatcga gattgccgag aataaaaccg cctccatgca
    18481 aataaaatag cgtcgctggg ctatctggct gcggacaaaa gagacgtgtt tccacctgcc
    18541 catattttgt tggaaccatg taagctctgg ttgccatttc tggagcgccc gcattccaga
    18601 atcggcgctc aagcgtgtaa tactgtcgtt gctcagcaat cgttcccgtt gcgggccagg
    18661 gcggtaaatc cggctgaaga gtattcacaa cggtcttcat ttcagcagaa atgagctcca
    18721 gaacaggtaa tttgttttcc ggcttcatac aaaaactcct ttcaaattac gtcattgtaa
    18781 ggaaccactg ccatgaaaat gcgatcccgc ctgctgatat tgaaactggc tgcgtctcgc
    18841 gcgctcccgt cagattgtgt taacattcgc cgctcagtta accacccgta aaaacaacca
    18901 tgaaatttcc cggtaaacgt aaatccaaac attacttccc cgtaaatgca cgcgatccgc
    18961 tgcttcagca attccagcca gaaaacgaaa ccagcgccgc ctgggtagtg ggtatcgatc
    19021 aaacgctggt cgatattgaa gcgaaagtgg atgatgaatt cattgagcgt tatggattaa
    19081 gcgccgggca ttcactggtg attgaggatg atgtagccga agcgctttat caggaactaa
    19141 aacagaaaaa cctgattacc catcagtttg cgggtggcac cattggtaac accatgcata
    19201 actactcggt gctcgcggac gaccgttcgg tgctgctggg cgtcatgtgc agcaatattg
    19261 aaattggcag ctatgcctat cgttacctgt gtaatacctc cagccgtacc gatcttaact
    19321 atctacaagg cgtggatggc ccgattggtc gttgctttac gctgattggc gagtccgggg
    19381 aacgtacctt tgctatcagc cctggccaca tgaaccagct gcgggctgaa agtattccgg
    19441 aagatgtgat tgccggagcc tcggctctgg ttctcacctc ttatctggtg cgttgcaagc
    19501 cgggtgaacc catgccggaa gcaaccatga aagccattga gtacgcgaag aaatataacg
    19561 taccggtggt gctgacgctg ggcaccaagt ttgtcattgc cgagaatccg cagtggtggc
    19621 agcaattcct caaagaccac gtctctatcc ttgcgatgaa cgaagatgaa gccgaagcgt
    19681 tgaccggaga aagcgatccg ttgttagcat ctgacaaggc actggactgg gttgatctgg
    19741 tgctgtgcac cgccgggcca atcggcttgt atatggcggg ctttaccgaa gacgaagcga
    19801 aacgtaaaac ccagcatccg ttgctgccgg gcgctatagc cgaattcaac cagtatgagt
    19861 ttagccgcgc catgcgccac aaggattgtc agaatccgct gcgtgtctat tcgcacattg
    19921 cgccgtacat gggcgggccg gaaaaaatca tgaacaccaa cggagcaggg gatggcgcac
    19981 tggcagcgtt gctgcatgac attaccgcca acagctacca ccgtagcaac gtaccaaact
    20041 ccagcaaaca taaattcacc tggttaactt attcatcgtt agcgcaggtg tgtaaatatg
    20101 ctaaccgtgt gagctatcag gtactgaacc agcattcacc tcgtttaacg cgcggcttgc
    20161 cggagcgtga agacagcctg gaagagtctt actgggatcg ttaagttatc gtcggttcgt
    20221 aggccagata aggcgttcac gccgcatctg gcatttggca tctcgatgcc tgatgcgacg
    20281 ctggcgcgtc ttatcatgcc tacatatttt tcatatttta catccggcaa ccaccgttta
    20341 ccccgtcacc acctcacccg ccggtggcgt ttccagcagt tccagcatag tacgggcgat
    20401 ttcacgctcg cccatcacta cctgattcgc accacgttcg gtgatatacg ccacttcatc
    20461 gtcataatgg gcgcgggcaa taatctcaat atccggattt ttcgcgcggg cagatgccac
    20521 aatctcaccc gcttcataac cgttggggat cgtcaggatc agccattttg cacattccag
    20581 atgcgccagt tgcataattt cttcgttcgc cgcatttccc aatactgcgc ggaccccacg
    20641 ctctcgcagc tcatcaacac gggttcgtga cgtctcaatc accaccagcg gaatatcaga
    20701 ggcgagcaat ttctccccca gcaggctgcc tacacgaccg taacccacca gtagcgcatg
    20761 gttgcaaata tccactggga tctgcttctc ttcttcgatt gcctcttcca gcgtctgctc
    20821 ttccagcgtt tcggtcttcg ccagatattt ctccagtagt gcgaacagta ccgggttgag
    20881 cataatcgac aggatcgccc ctgccagtac caggttttgt ccggcctgcg gcagtaaatt
    20941 caatgccatt cccagtcccg ccaggataaa cgcgaactca ccaatttgcg ccaggctggc
    21001 ggcgatagtt aatgccgtac gttgggagtg accaaacagt cgcaccagga aaaacgcggc
    21061 taacgactta ccaaacagaa taatcgccag cgtcgccagc actgccagcg gttgctgaat
    21121 cagaattaac ggatcaaaca acatcccgac ggagacaaaa aacagcaccg caaacgcgtc
    21181 gcgcaatggc agcgtatcgt gggcggcacg gtgactcagt tcagactcgt tcagtaccat
    21241 cccggcaaag aacgcaccga gtgcaaagga gacatcaaac agctctaccg caccaaaggc
    21301 aatccctaac gccagcgcca gcaccgacag ggtaaacagc tcgcgagaac cggttgccgc
    21361 gctgcgcgcc ataatccacg gcaccagacg gcgacctacc agcatcataa tggcgataaa
    21421 cgcgatcact ttgccgatgg tgatccccat atcgactgca agagtggcaa agcccacatc
    21481 gccctgttcc atcattcctg ccactgcggg cagcaacacc agcgtcagaa ccattaccag
    21541 gtcttccaca atcaaccaac cgatggcgat ttgcccacgc tgactgtcaa ttaattgccg
    21601 ttcttcaagt gcgcgcagta acaccacggt actggcggtg gaaagacata aaccgaacac
    21661 gataccggtc attaacgacc agcccagcac ggcagagagc gccataccca gcagcgtcgc
    21721 cacggctatc tgggcgatcg caccgggaat ggcgatggcc tttaccgcca tcaaatcctt
    21781 cagcgaaaag tgcagaccca cgccaaacat caacagaatg acgcccagtt cagccagttc
    21841 cggcgcaagc ttggtatcgg caacaaagcc cggagtgaat ggtcctgcca gcacacccgc
    21901 taacagatat cccaccagag gagaaatacg tagtttattg gccagcatgc cgaggataaa
    21961 ggcgagcaca aggccgccaa caatggtggt gataagcggg gtggcgtgat gcattccgtc
    22021 tccttttcct ggtggttatt gtccattttt ggccgggaaa accaaaatta caggtaatag
    22081 tttatgacaa tttcattgat gatgttcatg aataattatt gaattttgca gaaaaatgga
    22141 attagctgca aaaaaagcac ggatcggaaa acagaaggcg gtttaacaga ggaaaggctt
    22201 atggcaactg taaaacgatg cagccaaagt ttgcctttgg ctgcaatgaa atcagtcttt
    22261 atgccggtta tcaggcagga atatggtcaa catccccaat agtggcagga aagcacagat
    22321 tttatagact aactcgatgc tggtgtgatc ggcgataagc cccagaactg ccgctcccag
    22381 acctcccatg ccaaaagcaa aaccgaaaaa gagtccagaa accataccga tacgtcccgg
    22441 aagcagctcc tgagcgtaaa ccagaatggc agagaatgcc gaagcgagga taaatccaat
    22501 aatcaccgtt aaaacccccg tccagtgcag gctggcgtag ggtaaaatca gcgtaaacgg
    22561 cgcaacgccg aggatagagc cccaaatcac atatttcctc ccaattttat cccctacagg
    22621 cccgccgatc accgtacctg ccgcaacggc aaacaggaag gcaaacagat gaagttgagc
    22681 attctggata gataatccga atttttgcat cagataaaag gtgtaatagc tgctgatgct
    22741 cgccatatag aaatatttcg agaaaatgag gattaacaga atgctgaccg ccagtacaac
    22801 tttattgcgc ggcagtggat tgataatcgt cgctttgggt tttcctttat tcattcggtg
    22861 ctgtgccgag taccaacggc tgatttgcgc caacaccacg atcgccagca gtgccgcaag
    22921 cacaaaccag gcaacgttgc ctttgccata aggcgcgata atcaccgccg ccagcaaggg
    22981 tcccagggaa ctgccaaagt tgccgccgac ctgaaagatt gattgcgcca ggccatgccg
    23041 cccgccagaa gccatacggg ccacgcgaga agattccgga tgaaagaccg atgaaccggt
    23101 accgaccagc gccgccgcca gcagaactgc gccaaaactg ccagccagcg caagcagcac
    23161 cagaccactt aaggtaaagc acatgccaat tggcaacgac cacggcatcg gatatttatc
    23221 ggtccagtag ccgaccactg gttgcagtag cgaagaggcg agctggaagg tgagggttat
    23281 catgccaatc tgcataaatg tcagagaaaa ttctgactga agcagcggat aaatcgccag
    23341 aatcagcgat tggatcatgt cgttcagcag atgtgagagg ctgatagcac ctaaaatacc
    23401 aaacgatgtt cgggccttgg tcgttgacgc agccgcgccc gccacaggct gggtttgttc
    23461 actcattgcc ataggaaagt cactttttca gggttgcgat gtaaagaatg atcttatttg
    23521 tgattattac ccgactaaca tacctgtatg cgccgcctga aggaagtctc aacgctgaat
    23581 acagaatttc taatctggat gcagatttat cttcaccgga cgcagacttg tctatgatgt
    23641 cgcgtcatac tatttttcaa cacgttgaaa tcaggtcagg gagagaagta tgaaattatt
    23701 gcagcggggc gtggcgttag cactgttaac cacatttaca ctggcgagtg aagctgctct
    23761 ggcgtatgag caggataaaa cctacaaaat tacagttctg cataccaatg atcatcatgg
    23821 gcatttttgg cgcaatgaat atggcgaata tggtctggcg gcgcaaaaaa cgctggtgga
    23881 tggtatccgc aaagaggttg cggctgaagg cggtagcgtg ctgctacttt ccggtggcga
    23941 cattaacact ggcgtgcccg agtctgactt acaggatgcc gaacctgatt ttcgcggtat
    24001 gaatctggtg ggctatgacg cgatggcgat cggtaatcat gaatttgata acccgctcac
    24061 cgtattacgc cagcaggaaa agtgggcaaa gttcccgtta ctttctgcca atatctacca
    24121 gaaaagtacc ggcgagcgcc tgtttaaacc atgggcgctg tttaagcgtc aggatctgaa
    24181 aattgccgtt attggtctga cgacggatga cacagcgaag ttaggcaacc cagaaaactt
    24241 caccgatatt gagttccgta agcccgccga tgaagcgaag ctggtgattc aggagctgca
    24301 acagacagaa aagccagaca ttattatcgc ggcgacccat atggggcatt acgacaatgg
    24361 tgagcacggc tctaatgcac cgggcgatgt ggagatggca cgcgcgctgc ctgccggatc
    24421 gctggcgatg atcgtcggtg gtcactcgca agatccggtc tgcatggcgg cagaaaacaa
    24481 aaaacaggtc gattacgtgc cgggtacgcc atgcaaaccg gatcaacaaa acggcatctg
    24541 gattgtgcag gcgcatgagt ggggcaaata cgtgggacgg gctgattttg agtttcgtaa
    24601 tggcgaaatg aaaatggtta actaccagct gattccggtg aacctgaaga agaaagtgac
    24661 ctgggaagac gggaaaagcg agcgcgtgct gtacacccct gaaatcgctg aaaaccagca
    24721 aatgatctcg ctgttatcac cgttccagaa caaaggcaaa gcgcagctgg aagtgaaaat
    24781 aggcgaaacc aatggtcgtc tggaaggcga tcgtgacaaa gtgcgttttg tacagaccaa
    24841 tctggggcgg ttaattctgg cagcacaaat ggatcgcact ggtgccgact ttgcggtgat
    24901 gagcggaggc ggaattcgtg attctatcga agcaggcgat atcagctata aaaacgtgct
    24961 gaaagtgcag ccattcggca atgtggtggt gtatgccgac atgaccggta aagaggtgat
    25021 tgattacctg accgccgtcg cgcagatgaa gccagattca ggtgcctacc cgcaatttgc
    25081 caacgttagc tttgtggcga aagacggcaa actgaacgac cttaaaatca aaggcgaacc
    25141 ggtcgatcca gcgaaaactt accgtatggc gacattaaac ttcaatgcca ccggcggtga
    25201 tggatatccg cgccttgata acaaaccggg ctatgtgaat accggcttta ttgatgccga
    25261 agtgctgaaa gcgtatatcc agaaaagctc gccgctggat gtgagtgttt atgaaccgaa
    25321 aggtgaggtg agctggcagt aatccgaaag tgccggatgt tcgcatccgg cacaatgctt
    25381 aatcgcggcg ggcgatatca gcaaatttgg catcgaggat cttcgccaga tcgcctgccg
    25441 ccagttcgat atccagcccg cgcttaccgc cggaaacata aatagtggca aattcttgtg
    25501 caggggcgtc gataatcgtt ggcagacgtt ttttctgccc cagtgggcta atccccccta
    25561 ccagataccc cgtcgaacgc tgcgcggcca tcggatcggc catctcaact ttcttggcac
    25621 ccagcgcttt tgctactttt ttaaggtcca gttgacctgc gaccggcgta acggccacgg
    25681 caaggtgttt catatcaccg ttcactgcga ccagcagcgt tttgtagacc tgatccggat
    25741 tcaaacctaa ttttttgacg acttcatcgc caaaattggt ttcagccgga tcgtgctcgt
    25801 aggtatggat ttgaaacgaa atcttgtttt tttcgagtaa tttaactgcg ggtgtcatgg
    25861 taattttcct gccttaaaca aaaaaacgaa gcaagcatac gcctgatggc ggtccaaaaa
    25921 aaagagtcat cttgcctaag agtattggca ggatggtgag attgagcgac aatcgagtta
    25981 caccgtcgct taaagtgacg gcataataat aaaaaaatga aattcctctt tgacgggcca
    26041 atagcgatat tggccatttt tttagcgcaa catttgcggc aaattccctt ctccatacag
    26101 gtgtagtgca ccgaccgcga ccacatatcg ccccggcggc atggcgcgta atttatcccg
    26161 ccaggcgaga tttcgctgat gcatcagcac atcgtacagc gactgactga acgtattggg
    26221 cagcgttatt tcattatttt gcggcggcgc attcagccac cagctcatca tttgttgcag
    26281 caaccgtgcg ttggtatgcc agtgggtcag cgtatcgtcc agcagtgcca gtcctttgtc
    26341 agggagctgg agcaacatgg taatctggtt ttcagcccct tccagttcaa tcacgggttt
    26401 atgttgttgc ttcgccgcct gcaatagctg gtaatcgata ccgtattctg cccgcagccc
    26461 cagtttttgt gcctgcgtcg cctgaagaac catcgcgatt tgccacagcg gttgggtaga
    26521 aaagagtgag ggagaaatgc ccatctcctg gctaatgtgc tgcagttttt ggagttgctc
    26581 ctcgctaatg cgctcttcca gcgcctcgca ggcaggcaaa ttagcaaaag gcgtatcgtt
    26641 gttggaaaca tccgcctcga cgatcagcgc atcggcgttt ttgagctttt tgagcaaacg
    26701 ggcgggcagg ggagccatat cgtggctacc catatgaata ctgccaatca gatgaaaatg
    26761 gcgattgccg gggagggtga tatcgatggc aggccaggag taatgattac cgcgcagcgc
    26821 ggcccaaagc gtttttaccc ggtacaacag atccatacga cctccctttg tgaaatatca
    26881 tgctagcgcg cggggaggga tggcgcaaat ggagttggga acttgtttaa tgtgtttgta
    26941 tgattccgta tgttcttgta tcgctattca cagggaagca acagttaaaa actatgaaac
    27001 aggcaacaag aaaaccgacg acacccggag atattcttct ctatgaatat ctggaaccgc
    27061 tagatttgaa aatcaatgag ttagcagagt tgctgcatgt tcatcgtaat agcatcagtg
    27121 cactgatcaa taacaatcgt aaactcacca ctgagatggc attccgtctg gcgaaagttt
    27181 ttgataccac agtcgatttt tggctaaacc tccaggcggc agttgatctt tgggaagttg
    27241 aaaacaacat gcgtacccag gaagaattgg gacggattga aacagtggct gaatatttgg
    27301 cacgccgtga agagcgtgca aaaaaggtcg cgtaaggcca aaacatcgct tcatctcgta
    27361 ggccggataa ggcgttcacg ccgcatccgg catccgagca ccattgcctg atgcgacgct
    27421 tacgcgtctt atcaggccta caaacctgtg cctgaaccgt aggccggata aggcgttcac
    27481 gccgcatccg gcagtcatgc gtcgatgcca aatgcgccac cctaaagcag cgcatccgca
    27541 atgatgtact tattccttcg gtttaaaccg cagcaaccgg ttggcgttac tcactacggt
    27601 aatcgacgag agcgccattg ccgctccggc aactaccggg ttaagcagtg ttccagtaaa
    27661 cggccacaaa ataccggcgg cgaccggaat accgatactg ttgtagataa acgcgccgag
    27721 caggttctgt ttcatgttgc gcagcgttgc gcgggaaata gcgagcgcat ccgcaacgcc
    27781 catcaggcta tggcgcatca gggtaatcgc cgcggtttca atagcaacat cactgccgcc
    27841 acccatcgca atgccgacat ccgcctgagc cagcgctggc gcgtcgttaa tgccgtcgcc
    27901 caccattgcc acctgacgtc cttcactttg caggcgttta atcgcttcgg ctttaccgtc
    27961 cggcagcacc ccggcgatca cctcatcaat ccctgcttct ttggcgatcg cattggcggt
    28021 gattgggtta tccccggtca acatcaccag acgatatccc gctttatgca gacgttgcag
    28081 cgccgccacg ctatcactac gcaacggatc gcgtacagcc agcagggcta ccgctttccc
    28141 gtcaaccgcc agcagcacag gcgttgcccc ttgcgatgcc tgagctgaaa tatctgcttc
    28201 aatcgcttta gtatcaactt gctggtcatt taacagcgcc tgattgccca gcaataacgc
    28261 atgaccttca gcttcaccgc tcacgcccag cccgcgcaat gtgcggaaac cgttgacctg
    28321 cggcagctgc atatcgcttg ctttatcgag gatcgcccgt gccagcggat ggctggaacc
    28381 ttgctccagc gccgccgcca gacgcagcgc ctgcgcttcg tcaaaatcaa caaatgtttt
    28441 cactgcgaca acctgcggct tcccttcagt cagcgtcccg gttttatcga acactacagt
    28501 gtcgagtgta ctggcgcgtt gcagcgcgtc ggcgtcccgc accagcacgc caaactcagc
    28561 cgcccgcccg acgccggaaa taatcgacat cggcgtcgcc agccccagcg cacacggaca
    28621 ggcgataatc agtaccgtgg tggcaatcac cagggtatag acaatctgcg gtgccggacc
    28681 aaagaaatac cagattgccg cactgacaag cgcaataacc actactaccg gcacaaatac
    28741 ggctgagatt ttatccgcca gctgaccgat ttctggcttg ctgctctggg cctggcgcac
    28801 catacgaatg atccttgata gcgtggtatg gctaccaacc gcactggcgc gaaacagcac
    28861 gctaccgtcc tgcaccactg tcccggcatg gacgctatcg ccttcgcctt tttgctgcgg
    28921 gataggttcg cccgtcagca tcgcttcatc cagccatgct tcgccctggg taatctcgcc
    28981 atctactggc acgcggtcgc ccgtcgtcag gcgcagtagc atgcctggct gaacttccgc
    29041 cagcggcacg tttttttcac cttcgtcagt aaccaggcgc gccgtcggtg gggttaaatc
    29101 gagtaatttc tccagcgcct tagaagaacg ctgacgtgcg cgcgcttcca gcatatgacc
    29161 gagattaatc agaccgataa tcatcgcact ggcttcatag taaagatgcc gcgcttccat
    29221 cgggaaccac tgcggccaca ggttgacgct catcgaatag agccacgcca cgccagtacc
    29281 cagcgccacc agtgtatcca tcgtcgctgc accgttcagc aggcttttcc atgcgctgcg
    29341 gtaaaaatgg ccgccggcga aaaccatcac tgccagggtt atcaggccga taaccaacca
    29401 caggctgcgg ttgtcagcgg tgaccatcat gttatcgccg atcatccccc agaccattac
    29461 cgggataccc acagccagtg caacaattgc ctgccagcgg aagcgcttca tcgtagcgac
    29521 ggcggtttct tgctggcgct cgcggcgttt agcgtcatct tcaatcgctt ccgcgccgta
    29581 gcccgctttt tccaccgcct gcactaaatc ttgtggggag gcactgccca tcaccagcgc
    29641 agtacgctcc gccaggttta cccgtgcctg agtgacgccc ggtacgcttt gcagcgcatt
    29701 ttgtacgcgg gtgacacagc tggcgcagct catgccgctc agcagcaact gctggctgtc
    29761 atcgtcatcg gcggtcgctg ccggaagcgc ctcagaaacc gctgtcagtg cttccgacgg
    29821 gattgatgac tccgccagcg gtttagcctt tgggtggctt acagatgcgt cataacccgc
    29881 ttgtttgatg gtttcgatca gctgttctgc actggcagtc ccggtaacgt gcgcttcagt
    29941 gatagacaca tccgcttgtt ccacatccgg gcgctgttcg agactttctt tcacgcgttt
    30001 aacgcagtga ccgcaggaca ggccgtccag ggtcaggtcg atagtttgtg acataaaaca
    30061 ctcctttaag acagttttga ctggctgtga taaaggttaa accttccagc aaggggaagg
    30121 tcaagaaatt aataacccag gcgagtaaaa gtccgtaaag attaaaaaat cggctcgatt
    30181 tgcatcagga ttagacattt atctctttgt tttcctgtag ttaagttgcg ggtgctaagt
    30241 taaatcagga tgcctgaaaa tcggcaccgg ggtgaggaat tacctcccgc atctataaaa
    30301 aggagttaac aaaagatgtt agatgcaaac aaattacagc aggcagtgga tcaggcttac
    30361 acccaatttc actcacttaa cggcggacaa aatgccgatt acattccctt tctggcgaat
    30421 gtgcctggtc aactggcagc agtggctatc gtgaccagcg atggcaacgt ctatagtgcg
    30481 ggtgacagtg attaccgctt tgcgctggaa tccatctcga aagtctgtac gttagccctt
    30541 gcgttagaag atgtcggccc gcaggcggta caggacaaaa ttggcgctga cccgaccgga
    30601 ttgcccttta actcagttat cgccttagag ttgcatggcg gcaaaccgct gtcgccactg
    30661 gtaaatgctg gcgctattgc caccaccagc ctgattaacg ctgaaaatgc cgaacaacgc
    30721 tggcagcgaa ttttacatat ccaacagcaa ctggctggtg agctggtagc gctctctgac
    30781 gaagtcaacc agtcggaaca aacaaccaac ttccataacc gggccattgc ctggctgctg
    30841 tactccgccg gatatctcta ttgtgatgca atggaagcct gtgacgtgta cacccgtcag
    30901 tgctccacgc tcatcaatac tgttgaactg gcaacgcttg gcgcgacgct ggcggcgggt
    30961 ggtgtgaatc cgttgacgca taaacgcgtt cttcaggccg acaacgtgcc gtacattctg
    31021 gccgaaatga tgatggaagg gctgtatggt cgctccggtg actgggcgta tcgtgttggt
    31081 ttaccgggca aaagcggtgt aggtggcggt attctggcgg tcgtccctgg agtgatggga
    31141 attgccgcgt tctcaccacc gctggacgaa gaaggcaaca gtgttcgcgg tcaaaaaatg
    31201 gtggcatcgg tcgctaagca actcggctat aacgtgttta agggctgatc atgatgaaca
    31261 cggaaggtaa taacggtaac aaacccctcg gtctatggaa cgtcgtttcc atcggcattg
    31321 gggcaatggt gggggcgggg atcttcgcgc tgctggggca ggctgcgttg ctaatggaag
    31381 cctcgacctg ggtcgccttt gcttttggcg gtattgtggc gatgttttcc ggttatgcct
    31441 atgcgcgtct gggggcgagc tatcccagca atggcggcat tatcgacttc tttcgtcgcg
    31501 gattaggcaa cggcgtcttt tcgctggcgc tctcgttact gtacctgttg acgctggcgg
    31561 tgagcatcgc catggtcgcc cgtgcttttg gcgcttatgc cgtgcagttt ttgcatgaag
    31621 gcagccagga ggagcacctt attttgctct acgcgttggg gatcattgcg gtgatgacgc
    31681 ttttcaactc cttaagcaac catgcggtag ggcggctgga ggtgatcctc gtcggcatta
    31741 aaatgatgat cctgttattg ctgattattg ccggtgtctg gtcgctgcaa ccggcgcata
    31801 tttccgtctc tgcgcccccc agctccggtg cgttcttctc ctgtatcggg ataaccttcc
    31861 ttgcctatgc gggctttggc atgatggcga acgcggcgga taaagtgaaa gatccgcagg
    31921 tcattatgcc acgggcgttt ctggtggcaa ttggcgttac cacgttgctt tatatctcgc
    31981 tggcgctggt tttgcttagc gatgtatcgg cattagagtt agaaaaatat gccgataccg
    32041 ccgtagcgca ggcagcttct ccgctgctcg ggcatgtggg ttatgtgatc gtcgtcatcg
    32101 gcgctttact ggcgacggct tcagccatca acgcgaacct gttcgccgtg tttaacatca
    32161 tggacaacat gggcagcgaa cgcgaactgc cgaagctaat gaataaatcc ctgtggcggc
    32221 agagtacctg gggcaacatc attgtcgtgg tgttgattat gctgatgacg gcggcactaa
    32281 atttaggctc actcgccagc gttgccagcg ccaccttttt gatttgctat ctggcggtgt
    32341 ttgtggtggc aatccgcctg cgtcatgata ttcacgcctc gttgccgatt cttatcgtag
    32401 gtacgttggt gatgttgttg gtgatcgttg gctttatcta cagcctgtgg tcccagggca
    32461 gccgtgcgtt gatatggatt attggtgcaa tcttactcag ccttattgtg gcaatggtca
    32521 tgaagcgcaa taaaaccgta taacacctct ctgcgcgcag tactgcctgt actgcgcctt
    32581 tgtctttaat tcaaattacc tttcttattc ctttaataaa taagtcacaa aaaaatgata
    32641 tat
//
LOCUS       DAZUCE010000027        32295 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZUCE010000027 DAZUCE010000000
VERSION     DAZUCE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.027"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(169..2028)
                     /gene="gss"
                     /locus_tag="ACHY03_001129"
     CDS             complement(169..2028)
                     /gene="gss"
                     /locus_tag="ACHY03_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFS3904388.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELCWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHYRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2233..3099
                     /gene="yghU"
                     /locus_tag="ACHY03_001130"
     CDS             2233..3099
                     /gene="yghU"
                     /locus_tag="ACHY03_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFS3904389.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3222..3470)
                     /gene="hybG"
                     /locus_tag="ACHY03_001131"
     CDS             complement(3222..3470)
                     /gene="hybG"
                     /locus_tag="ACHY03_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFS3904390.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3483..3824)
                     /gene="hypA"
                     /locus_tag="ACHY03_001132"
     CDS             complement(3483..3824)
                     /gene="hypA"
                     /locus_tag="ACHY03_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFS3904391.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3817..4305)
                     /gene="hybE"
                     /locus_tag="ACHY03_001133"
     CDS             complement(3817..4305)
                     /gene="hybE"
                     /locus_tag="ACHY03_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFS3904392.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4298..4792)
                     /gene="hybD"
                     /locus_tag="ACHY03_001134"
     CDS             complement(4298..4792)
                     /gene="hybD"
                     /locus_tag="ACHY03_001134"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFS3904393.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPYIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4792..6495)
                     /gene="hybC"
                     /locus_tag="ACHY03_001135"
     CDS             complement(4792..6495)
                     /gene="hybC"
                     /locus_tag="ACHY03_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFS3904394.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6492..7670)
                     /gene="hybB"
                     /locus_tag="ACHY03_001136"
     CDS             complement(6492..7670)
                     /gene="hybB"
                     /locus_tag="ACHY03_001136"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFS3904395.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7660..8646)
                     /gene="hybA"
                     /locus_tag="ACHY03_001137"
     CDS             complement(7660..8646)
                     /gene="hybA"
                     /locus_tag="ACHY03_001137"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFS3904396.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8649..9767)
                     /gene="hybO"
                     /locus_tag="ACHY03_001138"
     CDS             complement(8649..9767)
                     /gene="hybO"
                     /locus_tag="ACHY03_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFS3904397.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9956..10243)
                     /gene="yghW"
                     /locus_tag="ACHY03_001139"
     CDS             complement(9956..10243)
                     /gene="yghW"
                     /locus_tag="ACHY03_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFS3904398.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10362..11249)
                     /gene="yghX"
                     /locus_tag="ACHY03_001140"
     CDS             complement(10362..11249)
                     /gene="yghX"
                     /locus_tag="ACHY03_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFS3904399.1"
                     /translation="MPRLIAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPARMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11406..12446
                     /gene="gpr"
                     /locus_tag="ACHY03_001141"
     CDS             11406..12446
                     /gene="gpr"
                     /locus_tag="ACHY03_001141"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFS3904400.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12486..12980)
                     /gene="yqhA"
                     /locus_tag="ACHY03_001142"
     CDS             complement(12486..12980)
                     /gene="yqhA"
                     /locus_tag="ACHY03_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFS3904401.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13171..14055
                     /gene="yghA"
                     /locus_tag="ACHY03_001143"
     CDS             13171..14055
                     /gene="yghA"
                     /locus_tag="ACHY03_001143"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFS3904402.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14327..14752)
                     /gene="exbD"
                     /locus_tag="ACHY03_001144"
     CDS             complement(14327..14752)
                     /gene="exbD"
                     /locus_tag="ACHY03_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFS3904403.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14759..15493)
                     /gene="exbB"
                     /locus_tag="ACHY03_001145"
     CDS             complement(14759..15493)
                     /gene="exbB"
                     /locus_tag="ACHY03_001145"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFS3904404.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15745..16932
                     /gene="metC"
                     /locus_tag="ACHY03_001146"
     CDS             15745..16932
                     /gene="metC"
                     /locus_tag="ACHY03_001146"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFS3904405.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            17072..17731
                     /gene="yghB"
                     /locus_tag="ACHY03_001147"
     CDS             17072..17731
                     /gene="yghB"
                     /locus_tag="ACHY03_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFS3904406.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17771..18670)
                     /gene="yqhC"
                     /locus_tag="ACHY03_001148"
     CDS             complement(17771..18670)
                     /gene="yqhC"
                     /locus_tag="ACHY03_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFS3904407.1"
                     /translation="MKREEICRLLANKVNNLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18864..20027
                     /gene="yqhD"
                     /locus_tag="ACHY03_001149"
     CDS             18864..20027
                     /gene="yqhD"
                     /locus_tag="ACHY03_001149"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFS3904408.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20132..20959
                     /gene="dkgA"
                     /locus_tag="ACHY03_001150"
     CDS             20132..20959
                     /gene="dkgA"
                     /locus_tag="ACHY03_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFS3904409.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21159..22085
                     /gene="yqhG"
                     /locus_tag="ACHY03_001151"
     CDS             21159..22085
                     /gene="yqhG"
                     /locus_tag="ACHY03_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFS3904410.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITETD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFIAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRIDEIRVLEKTADSARIHIRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22136..22393
                     /gene="yqhH"
                     /locus_tag="ACHY03_001152"
     CDS             22136..22393
                     /gene="yqhH"
                     /locus_tag="ACHY03_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFS3904411.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22436..24655)
                     /gene="ygiQ"
                     /locus_tag="ACHY03_001153"
     CDS             complement(22436..24655)
                     /gene="ygiQ"
                     /locus_tag="ACHY03_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFS3904412.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKAPSMQSRPVNTGAKKRPKAAVGR"
     gene            complement(24766..26178)
                     /gene="ftsP"
                     /locus_tag="ACHY03_001154"
     CDS             complement(24766..26178)
                     /gene="ftsP"
                     /locus_tag="ACHY03_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFS3904413.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26253..26990)
                     /gene="plsC"
                     /locus_tag="ACHY03_001155"
                     /gene_synonym="parF"
     CDS             complement(26253..26990)
                     /gene="plsC"
                     /locus_tag="ACHY03_001155"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFS3904414.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27224..29482)
                     /gene="parC"
                     /locus_tag="ACHY03_001156"
     CDS             complement(27224..29482)
                     /gene="parC"
                     /locus_tag="ACHY03_001156"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFS3904415.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29620..31227)
                     /gene="ygiS"
                     /locus_tag="ACHY03_001157"
     CDS             complement(29620..31227)
                     /gene="ygiS"
                     /locus_tag="ACHY03_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS3904416.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTQLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEIMDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSVTTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNNASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(31336..31818)
                     /gene="ygiV"
                     /locus_tag="ACHY03_001158"
     CDS             complement(31336..31818)
                     /gene="ygiV"
                     /locus_tag="ACHY03_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS3904417.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(31871..32263)
                     /gene="ygiW"
                     /locus_tag="ACHY03_001159"
     CDS             complement(31871..32263)
                     /gene="ygiW"
                     /locus_tag="ACHY03_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFS3904418.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
BASE COUNT         7881 a         8684 c         8117 g         7613 t
ORIGIN      
        1 taagcattca ttaaaaatct ttagaaataa ttagaacaca ggcattctgc tggttaatgt
       61 attcacatat gaaatgaata aatatttgaa tatataaata ttgaatattt tgattaatcc
      121 ccgtactgac tattcttcat aatcagtacg ggattgctac aacatgtatt actctttcac
      181 cacaattaac ggttcaatat cactctcttt tttgatgacc agtgattcat caccgcgcaa
      241 acacgtccca ccgtagttgc cgccaacggt gaaggtacat acctgaatgt atttaccgtc
      301 cactttcggc aaacaccaca gttgctgata gatgtttttc tgctcggcaa atttaccgct
      361 ggttttgtcc agcacttctt catgatggct gaccaggtcg atattgctac cacagcgacc
      421 ggcgatcggt ttcactgcgt aacctgtttt caccaattca tcattaacag tgaaatcggt
      481 atccagcagg taacgatagt gcgggaacag cgaccagagg atcggcagaa tcgctttgtt
      541 gccggggatc accgtccaca gcggctcaaa gaccagcact tccgggcgca gcaatacgtc
      601 gataagacgc acttcgtttt gcggatgacc ggtacggatc ggcaccgcag caaactcacg
      661 gtcgctaact tcacgaatct gatcaaacgc ggtttcccac gcccaggttt tccacacgca
      721 gttaaccagc cgtccttccc catcaatcag ttgcccggca gcatcccagc acagttcatc
      781 cagcccgcgc aagatacgcg tttcaaagcc cgcctggtgc agcgcctgct ccataaactg
      841 cgcgtgatag ttttcctcga tatctttgtc ctgcatgata tggacaaacg gacgtgcacg
      901 actgtgtttc caggcaccag ccagttcgtt aatcagccct tccgccggat tgaagccgtt
      961 gcctttatag ccctgctccg cccaacgttc gaggatcaag cccgcttcgg tatgacatga
     1021 ggcggagtcg gcgttgtact cgtaaacctt cagaccacgc tcatccatgc agaaatccat
     1081 acgaccggtg atcatatggt gacggcgacg ctgccaggag agacgcaaac gtggccagag
     1141 gattttcgga atgtcgaaca gcgccagcag gttgtcatct ttcagcacct tgtcggttgc
     1201 gtgaagatac atcaggtgca gctcgttggt ggctttaatc agctcctgct cggcgctctc
     1261 ggtaatggtg tagtaatgat acggatcctg attgatcacc tgaccgttgg cctgcacata
     1321 ggcgttttgc agcggatctt tttcatccag ccatttaccg tcaaactggc ctttgttttc
     1381 cagacgcgct ccgctgattt tcagcagctc gcctgcaatt tccggctgcg gtaagctgta
     1441 ttcagtatct tccgtctgga tcatccagcc cagaatggtg gtgtcatcaa aagtgtcttt
     1501 aagggtatag cagccgtttt cgaccaccat ctccagttcg cgcgtccact gttgcccttg
     1561 cggcaacggg gaatgaatca cgttctgttc cgcaatacgg actttgttgc catgcaattg
     1621 ggtaatgatg gcgacatggc cagtgtcttt aaattcaccg cctttatccc agataagaag
     1681 cgcacccgcg accggcgcac gcggcgagcc gttaggaaat gcctgcaatg gcaggatgtt
     1741 gtcattaacc acttcacgca ggaagcgcag cgagaaaatc tcccacgcca tacccacgtc
     1801 agtgaagacc acaccgtaat tcagaaagag aaaacggcga gcaaattcaa cgcattgcca
     1861 tttgtggccc atatattcgt cgtcgatata gctacggaat acggcgtcat cttcgtattc
     1921 ctgcggatcg agagaactgt aatctgaaga gtagattgct accccacctg gggcgtagcc
     1981 caataatgtc ccgaacgggg catcctggct ggtcgttcct ttgctcatca ctttaccttt
     2041 aacaatacaa cctaagcagt tggcgtaatt gttgtgctct gattacctgc tcagcgacat
     2101 taaccggaca gaggtcagac taatcataca cctcatcgca gcggctggcg caaagggtta
     2161 aaaaattcac attctgtaca gcaagtgacc tgctacactg cttcaacact accactcaga
     2221 aggcaactca ctatgacaga caatacttat cagcccgcga aagtctggac gtgggataaa
     2281 tccgctggcg gcgcgttcgc caatatcaat cgcccggttt ctggtccgac gcatgaaaaa
     2341 acgctgcccg ttggcaaaca tccattgcaa ctttattcgc tgggaacgcc gaacggtcag
     2401 aaagtaacga ttatgcttga ggagctgctg gcgctgggcg ttactggtgc agagtacgac
     2461 gcgtggctga tacgcatagg cgatggcgat caattctcca gcggctttgt cgaagtgaac
     2521 ccaaattcga agatcccggc gctgcgcgat catacccata atccgccgat ccgcgtgttt
     2581 gagtcaggat cgattctgct ttatctggcg gagaaatttg gctacttcct gccgcaggat
     2641 ctggcaaaac ggactgaaac gatgaactgg ctgttctggt tacagggcgc ggcaccgttc
     2701 ctcggcggtg gttttggtca cttttaccat tacgcgccgg tgaaaattga gtacgccatc
     2761 aatcgcttta ccatggaagc caagcgcctg ctcgacgtgc tggataagca actggcgcag
     2821 cataagtttg ttgcgggcga tgagtacacc attgcggata tggcgatttg gccgtggttt
     2881 ggcaacgtgg tgctcggcgg cgtgtatgat gccgcagagt ttctggatgc gggaagttat
     2941 aaacatgtgc aacgctgggc gaaagaagtg ggcgaacgtc cggcagtgaa gcgtgggcgt
     3001 attgttaacc gcaccaacgg gccgctgaac gagcagttgc atgagcgcca tgacgccagt
     3061 gattttgaga cgaatacgga agataagcgt caggggtaag gattggtgtt cgtcgcagca
     3121 agccatcctg gccggataag gcgttcacgc cgcatccggc aatcgagcac aatgcctgat
     3181 gcgacgctgc cgcgtcttat cagacctacg aaagctaatc atcacgcgct ggtaatgtcg
     3241 taatccattt ggcgcagtgc gtctaatgtg gctttggctt catcttcgtc gatgatgctc
     3301 atggcaaatc cgacgtgtac cagcacccac tggcccagta gatcggcagg gttaccttca
     3361 caaatcaggg cgatattcac gtcgcgcttg ataccacata cttcaacctg cgcaagctgg
     3421 tgaatatctt caccgacagc cagcacctgg cctggaacgc caatacacat tgttaactcc
     3481 ggttattcaa cttcaatact tttgacgatc agcgaatcgc cggtatcgac acgcaaccgc
     3541 tcgccatgac agagcggaca ctgcgcatcg tgctgatgaa tctccaccac ctggctgcaa
     3601 tcccagcacc aggcctgggc gggtttatag acgatatgta aatcgcaccc ttgcgccacc
     3661 gttccgtggc agacaatttc aaaactaaaa cggacggcgc tctcctcaac gcaggagagc
     3721 gcgccaattt ccagccacac ggcggtgacg cgcttaacat cgtgctgctc cgcctgccgt
     3781 tggataattt caacggcact ctggcaaagc gacaactcat gcattttcgc cactcctgcg
     3841 accaaacagc agggcgcgac gccctgcgtg tggtacatcc ggattcgtga ctggcagcga
     3901 aaggatcatt cgtgcgcagt catccgtcag gcgttggccc tcttcaatcg acatactgtg
     3961 cgaaagcggt gacatcagcg aacaggagag gtattgcgaa acaccgtcca gttcgccaac
     4021 ggtaaaggtc atggtgccat acggcagttg cagaccgatt ttttcactca ctttgcgcag
     4081 cggccagagt tgatccgggc cggggaagat aactgcactc agcatccacg gggtgatcac
     4141 acaccccgtc cactgacctt cgaacagcgt aaaatcagaa acatacaccg gcattgaagg
     4201 atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt tcttcaaacg ctgcctgtac
     4261 ttgcgccttc ggggaggtct ggaaacctgc tatctcttca gtcatgaatc gcctcccgtg
     4321 ggatggcttc cacgccagat tcacgcagcg cagccagaac ctgctcaagc gcaggttcaa
     4381 tcattgcttc aaccgtcggc gttaagccga tgtatggctc cagcgattcc ggaattacgc
     4441 cgaccagggt cagctttttc ggaaactcgc cggtgaagcg cagggccgac aagacgtcgg
     4501 ccaggccaag ctgatgcgga gagattttgt tggtaaacaa cgccggaact tcttcatccc
     4561 gcaggatcat catcgttccc ggcgcgctct tcttcgacac aatggcatcc gcaataatca
     4621 gatgatcgcg atttgccatg tcgccaagta gctccattcc cgccgtgccg ccatcgagga
     4681 tctcaacata atccggcaga atgtatcgtt gctctaacgc ttcgacaatc cgcacaccga
     4741 tggcttcatc ggtcagcaaa atattgccga cccctaagac taaaatacgc attacagaac
     4801 cttcactgaa accacttcgt tgccgtcagc atccactacg tgtaccgcac aggccatgca
     4861 cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt ttattcggat cggcaaccgg
     4921 tgtacccacc agcgactgct cgtaaggacc gacgtcatca ttgaagttac gcggaccaga
     4981 gttccaggtt gatggaacaa ccgcctggta gttgctgatg ataccgtctt taataaccat
     5041 ccagtgagag agcataccgc gcggcgcttc gaggaagcca acacctttga actcacccgt
     5101 tgccggaatg ttcggcttca caaaggtggt gtgatcgcct ttgccgatat tggtgatcag
     5161 tgcactgtat tggttttgca ggatatcctg caattcacag cagtgaacgg tacgaccaat
     5221 aatacggccc agcgtggagt gcagctgtgc cacttccagc gtgttgccag tcagtttctg
     5281 ataaatcgca acgatttcat tcagtttgtt ctgggtagat tcgcgacctg ccgccagttt
     5341 caccagcata ttagccagcg gacccacttc taccgttttg ccgtagaaag tcggtgattt
     5401 cacccaggaa tatttcccgt cgtcagacca accatcataa gccggaatgg tggtaccttc
     5461 ccacggtgcc tgcggcgctt cgtctttata ccaggagtgc ttcgcgcttt cctgaatgcc
     5521 tttgatcagg tattcatcgg aatgagaagt gatcggacga tacgaggaca gatccgcatt
     5581 ctcaatgtag ccgcccggga acaggaagct gccgttttta ctgtcggtcg ggaattccgg
     5641 cacgctcagg tagttcaccg cacctttacc gcgcgtcagc cattccggat agaacgcggc
     5701 aataactgcg gtatcaacct tataaacctg ttcaacaaag tcgctcagct tgtcgatgaa
     5761 agacttgatg tacatcaggc gctcaaggtt cagcacgccc aaaccgtcga ggttgatcgg
     5821 gttcgcgaca ccacctaccg ccaggttctg aatgtgcggc gttttaccgc ccagcagcgc
     5881 cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc aggtagtgcg ctaccgcaat
     5941 caggttcact tccggcggca gtttcatcgc cgggtgaccc cagtagccat tggcgaaaat
     6001 acccaactga ccgctggcaa ccagatcttt aatcttgttc tgaactttgg tgaactcttc
     6061 cggactgttc agatgccagg tcgaaacgcc tttcagcatt tcggaggctt tggttgggtc
     6121 agcttgcagt gcagaagtga tgtccaccca gtccagcgcc gagagctgat agaaatgaac
     6181 gatatggtca tgcgtggtgt gcgcagccag aatgatgtta cggatgtatt gcgcgttaac
     6241 cggaacgtcg atattcagcg cactttctgc cgcacgaacg gaagacagcg cgtgagtggt
     6301 agtacatacg ccacagatac gttgcacaat catccatgca tcgcgcggat cgcggttttt
     6361 cacgatctct tccatgccgc gccacatggt accggaagcc catgctttcg aaacgacgcc
     6421 attttcgatt tcgcaatcga tgcgtaaatg cccctcaata cgggttaccg gatcaatagt
     6481 aattctctgg ctcatgcttt gctcgcctca tgacgattat gatcgttttg ttttaaagga
     6541 ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc aaatctcaat agccacaaaa
     6601 ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg cgtaaccgcc gcccgggttg
     6661 aatgccacca gcgaataggt cagacgccag gttgcacaac ctaacagtgc actcagtgct
     6721 gacaggaaca gcatgcggga atcattacgc agcttcgcca cacgcagaac gaccagcggg
     6781 aagagcatca gcaggacttc aatccagaac atcacggagt agaagtcacc ggcaaacgct
     6841 aacgacagct tgtcgcgata gataagctcg ccaaagcgca gcacgatgaa aatcgccagc
     6901 aacacactga tggtgttggt cagcttgacg aacaggctct tttcatccgg accgttgcca
     6961 cgcagacccg cctgcaccag tgaaccttca aagatgacaa tcgagaagcc catgatgaac
     7021 gccgtcagca gcgagaacag cggcaacatt tcatagctct gccacagcgg atgcaccttg
     7081 tagcccgccg agatcatcag cgaccccatt gaagactggt gcatggtcgg cagcagcgca
     7141 ccgagcgcga tgatgaagaa catcaccttg ttcagacgct gtagcgacac cttccagccc
     7201 agacgttcaa acagtgccgg agcaaactcc agagccatca cgccgatgta gatggtcata
     7261 cagaccgccg tctcgaacag taccgagttc acgttgaagt gacccggaat gtagaagtac
     7321 ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca aaccacccag tgagtaacca
     7381 aacagactcg ccaacagcgc cggacgcacc agcggatggt attgcccacg gttaaagacg
     7441 tataccgccc acgccagcgc ccagccgcca caggcaaagc cggtgccaat cagcaggtca
     7501 aacgcgatcc acacgcccca cggaaagccg ccgttcaggt cggagacaga gcccagaccg
     7561 aacaccagac gcttcacaat caggagcata cagatgacga ttaacggtcc aaaaatcatg
     7621 accggtttac tgatgatttt gccgcccagc ggttttggat catgactcat gatcgtctcc
     7681 tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc acggttaagc ccgccagcac
     7741 agccagtggt agcatcatgc ctttatacag ggtgtgttga atattttcgg aacgcgcacc
     7801 ggtagaaaga tcgtccagtt tcggcaggtc gagattttca taaggcacac ccgtcagtac
     7861 cagaacctga gtaccgccgc cctctttctc gccgtacaga tgcggataat atttcggcac
     7921 cgtatgcagg taagtgtctc cagatttcag cgtctgacgc ggatagtggt attcgctgcc
     7981 aggcttcagc gccagacgtt ttttcgcctc cgccatcagc tcttcacgcg taccgaaaat
     8041 caccgcgccc gccgggcaca cttctacgca gccaggcaga ccgcctttat cgagacgttc
     8101 cacacctttc tggttgcaca gctcgcactt atgtagcgca ccaaacgggt tgttgtagtc
     8161 gtacttcggc acgttgtatg ggcaggcgac catgcagtaa cggcagccgg tgcacacgtc
     8221 tttgtcgtaa tggacaatgc cggttttcgg atcttttttc agtgcagaaa ccgggcacac
     8281 agagacacag ttcggatcga cgcagtgcat acactgtttc ttaatgtacg cgtagccgtt
     8341 ctcctcctgg tctttgttga cccctgtgcc gctggtccac acctgaatga tgttattggt
     8401 atacggcgac agtttgtcgt tgttcgacca ggtctgttcc ccttgcgggt tacgttcagg
     8461 gaagttgata tcctgacact tggtgacgca agcctggcag cctacgcaca aggtcgagtc
     8521 gtacaacatc cccagcgatc ccggaattgg cgggcggttt tcagcagccg catgactgac
     8581 agacggcagc gcgcccgtca gcaatgcccc gcaggaggct gctttaataa aattacgtct
     8641 gttcacggtt attctccccg tgagtcagcg ttatctttct tttgctgacg acccagttca
     8701 cgcaccgcca tcacgctgac accggcaacc agcccaacca caccgccgag caaaccaata
     8761 gcgcctgcag agacgttgcc gccctcttta gcgttaacat ccggtttctg tgaacgcgga
     8821 gtctgatttt cgacgttggc aagctgatgg atgcctttat ggaagccgat accttcttcg
     8881 ttacagccat aacacgggtg accaatcgcc accggccaca caccgccaac atcgcagaat
     8941 tgcagcgttg agcagttgcc gtaagtttct ggccctttac agccgaggtg gtacaggcac
     9001 cagccttcgc ggtggccttc atcaccgaac tctttggcaa aacgacccgc atcgaagtgc
     9061 gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg cgaaggtcgg acggtttttg
     9121 tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg caacggtcgc gaggaagttg
     9181 tgcgggttcg gcgggcagcc cggaatattg ataacggttt tgcctggcag aacttcttgc
     9241 aagctgactg cgccagttgg gttaactcca gctgcggcaa caccgcccca cgcagagcag
     9301 gaaccgatag cgataatggc tgctgcgcct tccgccgctt tgcggatgtg atccacaatc
     9361 ggctcaccgg caaccatgca ataaataccg ttatctttta atgggatgga accatccacc
     9421 actaacacat actgcccttt gtacttctcg agagcgttat gtttgttctc ttcgacctga
     9481 tgaccgaagg cggcggaaag cacttcgtga tactccagag agatagtctc cagtacgagg
     9541 ttttctaccg ttggatgcgt tgcacgaagc agagattccg tacaaccggt gcactcctgc
     9601 gcgccaatcc agataactgg cggacgctgc gggttagtaa ccgattcggc catctctgca
     9661 gcggctttgc tacttaaccc catggtggcg gctaatgctg cacaaagctt catgaaatca
     9721 cgacggttaa tgccgtgaga atggatgagg gtgttatctc cagtcattta tagttattcc
     9781 gttgcgaaga cctggcatat attttgcctc aatcgcaaaa tcaataatgc gatcgatgcg
     9841 ccatttacca cacatttatt atggttatcg atatcatgat actgcggcgg ggcgctaaaa
     9901 cgaagggaag aagcattagt gtaattaatt aaagcaagat aatacgtatg tttgatcaat
     9961 tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga acaggaatta ttctcacgaa
    10021 agaaatccat taccatctct ttatccagtc catagcggcg cgtcagaata cccgcttccc
    10081 aggcgctaag ctggcgatct ccggtctttt cgtacatccg gtgtgagtag cctaatacaa
    10141 aaccgcgttt ataatcggca cagaattttg tcacattaac cgcactgtcg gcatgcgttg
    10201 cttttaatcc cgccattaag cctttaccaa aatggttatt catcgttcac ctcattcgct
    10261 atatatcgtc atatatagcg atattttagg caaagaacga cttttgcgca cgctattttt
    10321 tcatgcttgt ttaagacgta atcccgtaag agataaacct atcaggagag atatttatcg
    10381 aaccatttca gtgtcctttg ccaggcaaga tcggcggcag atttgtcata acggggcgtg
    10441 gaatcattat ggaatccgtg attaaccccc ggatagatat acgcctcata aaccttatta
    10501 ttggctttca acgccgcctc gtaagcaggc cagccctcgt tgattcgggt gtccagttcc
    10561 gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat cggcagtggg tgcctgacga
    10621 ccataaaacg gcaccgcgca ggccagttcc ggatacgcga cagccgccgc gttcgatacg
    10681 ccaccgccat agcaaaatcc ggtaataccc actttgcctg tcgcttgcgg atagcgttgc
    10741 ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac ctgctgttgc
    10801 agttcgcgac ctttatcatc atttcccgga taacctccaa cggaacttaa accgtcgggc
    10861 gcaagagcaa tgtatcccgc cttcgccact cgccgtgcca catcttcgat atacggattc
    10921 agtccacgat tctcatgcac caccaccacg gctggcgttt tgccgctcat ccttgcgggt
    10981 ttcaccagat aaccccgtac ctcgccgtga ccatttggcg aaggatacgt gatgtactca
    11041 gcaacaatct ccgggtcggt aaactctacc tgagtcgcca gcgcataatt tggcttgagc
    11101 aaatcaaaca acgctaatgc cgtcatcccg cctaccgcat acttcgccgc aaggttgagg
    11161 aactcacgtt tcgagatttt cccgtgagcg taatagtcgt agtaatccag caactcttgt
    11221 gggaaatctt tggcgattaa acgcggcatc attgcactcc tcagttggtg tttttttaag
    11281 caaagcataa gcacgtgttt ttgcccagtt tttcgtcact ctgtgagcca gactacggga
    11341 tacgcgctga tgaatcgcta aactagaaac attgtttcga aattgaatgg tggaaaggag
    11401 aggtcatggt ctggttagcg aatcccgagc gttacgggca gatgcagtac cgttattgcg
    11461 gaaaaagcgg tttacgcctg cccgcgttat cgctcggttt atggcacaat ttcggtcacg
    11521 ttaacgcgct ggaatcacag cgtgcgatcc tgcgtaaagc gtttgattta ggcattacgc
    11581 actttgattt agccaacaat tacgggccgc ctccaggaag cgcagaagag aactttggtc
    11641 gcctgctgcg ggaggatttt gccgcttatc gcgatgaact gattatctct accaaggctg
    11701 gctacgatat gtggcccggc ccttacggct ctggcggttc acgtaaatac ctgctcgcca
    11761 gcctcgacca aagcctgaag cgtatggggc ttgagtatgt cgatatcttt tactctcatc
    11821 gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc gctggctcat gcggtacaaa
    11881 gcggtaaggc gctgtatgtc gggatctcct cttactcgcc agagcggacg caaaaaatgg
    11941 tcgagttgct gcacgagtgg aaaattccgc tgttaattca tcaaccttcg tacaatttac
    12001 taaaccgctg ggtggataaa agcggcctgc tggataccct gcaaaataac ggcgtgggct
    12061 gcattgcctt tactcctctg gctcagggat tgctgaccgg aaaatatctc aacggcattc
    12121 ctgaagattc acggatgcat cgtgaaggga ataaagttcg tggcctgacg ccgaaaatgc
    12181 tcaccgaagc caacctcaac agcctgcgct tattgaatga aatggcacag cagcgtggac
    12241 aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga tgatcgcgtg acgtcggtat
    12301 tgattggtgc cagccgcgcg gagcaacttg aggagaacgt gcaggcgctg aataatctga
    12361 catttagcac cgaggagctg gcgcagattg atcagcatat cgccgatggc gagctgaatc
    12421 tgtggcaggc gtcttccgat aaatgacctg ttaataacgg gcgcgagaac cgcgcccgaa
    12481 tcagatcagt gattatgacg agttagtcgg tcaagatagc ccatcacaaa tgcagagagc
    12541 acaaacgtca gatggataat gacgtaccac atcagtttgt tatccgggac atttttcgca
    12601 tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca caatcgacgc tgctactttg
    12661 tttttcagcg acgttgcgtc cattttcccc agccagttca gcttctcttt gttctcggag
    12721 atatcgagtt gtgagacgaa attctcataa ccggaaaaca tcaccatcac cagcaaaccg
    12781 ccaaccagcg tcatatccac cagcgacagc aacacgagga tcaaatctga ttccgccatc
    12841 gagaagatat tcggcagtac gtgaataatc tcctggaaga acttcagcgc cagggcaact
    12901 aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc agcgagaagc atacattgca
    12961 ttttcaagaa aacgttccat acattcctgt cgtgtagtta aaacgggcaa cagtatatcg
    13021 caattgagta aacagcacgc aacagcaaat caacatatcc gttaaacctt tgtcgcataa
    13081 ttctgagggt tagggatctt cctggttccc ttatcaacgc atcgagagcg tctaccctta
    13141 aaggacgttg aacctgaagg gagaaaaacg atgtctcatt taaaagaccc gaccacgcag
    13201 tattacactg gtgaatatcc caaacagaaa caaccgacgc caggcatcca ggcgaagatg
    13261 acaccggtac ctgattgcgg cgagaaaacc tatgttggta gcggtcgcct gaaagatcgt
    13321 aaagccctgg tgacaggggg cgattccgga atcggtcgcg ctgccgccat cgcttacgcg
    13381 cgtgaagggg ctgacgtggc gatcagttat cttcccgtgg aagaagaaga cgctcaggat
    13441 gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc tgctgccagg cgatttaagc
    13501 gatgagaaat ttgcccgttc gctggttcac gaagcgcata aggccttagg tgggctggat
    13561 attatggcgc tggtcgccgg gaaacaggtt gccattcctg atattgcaga cctcaccagc
    13621 gaacagtttc aaaagacctt tgccattaac gttttcgcac tgttctggct aacccaggaa
    13681 gcgatacccc tgctaccgaa aggtgcaagt atcatcacca cttcgtcaat ccaggcatac
    13741 cagccaagcc cgcatttact ggactatgcg gctacgaagg cggcgattct gaactacagc
    13801 cgaggcctgg caaaacaagt cgcggagaaa ggtattcggg tgaatattgt cgcgccaggc
    13861 ccgatctgga cagcactgca aatttccggc ggacaaacgc aggataagat cccgcagttt
    13921 ggtcagcaaa cgccgatgaa acgtgcgggg caaccggcgg aactggcccc tgtatatgtt
    13981 tatctggcaa gtcaggagtc gagctacgtc accgcagaag tgcacggcgt gtgcggcggc
    14041 gagcatttag gttaaaaaaa agcccggttg tgaaaagcaa ccgggcatca ttgtgaattc
    14101 actcttacct gatgtggcga aaacgcctta tccgcctaca aacgcatgaa gattcaacat
    14161 gttgcaatag attttgtagg cctgataagc gtagcgcatc aggcattgag cacctaatgc
    14221 cggatgcggc gtgaacgcct tatccggcct acaaacgcac gaagattcaa catgttgcaa
    14281 tagattttgt aggcctgatg agcgtagcgc atcaggcaat tctactttac ttcgctttgg
    14341 cggtttcttc gccgaccaga cctatcttca ggtaacccgc ctgatgcagc gtatccatta
    14401 ccttcatcaa cgtctcgtaa tcgacggttt tatccgctcg gaagaagatg gtggtgtctt
    14461 tcttaccttc ggttaacgca ttcaacgccg taatcattgt ttcatcggtg accggatcgt
    14521 taccgataaa catcgagttg tctgccttca ccgacagata aaccggtttt tctgggcgcg
    14581 gctgcggtgt gctggtagaa gcaggcaagt tcaccttcac atctaccgtc gctaacggtg
    14641 ccgccaccat aaagataatc agcagaacca acatcacgtc gataaacggc gtcacgttga
    14701 tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag atgcattgcc atcggacatt
    14761 atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc agcggcgctg gcttccagat
    14821 ccaggtcacg gctttgcagc aataatacct gcgctgcaac atcacccagc atcgctttaa
    14881 agccgccaat ctgacgcgcg aatacgttat agataacgac cgccggaatc gctgccacca
    14941 gaccgattgc cgttgctaac agagcttctg cgatacccgg cgcaacgact gccaggttag
    15001 tagtctgcgt ttgcgcgata ccaataaagc tgttcatgat gccccagacc gtaccaaaca
    15061 gcccaacgaa cggcgaaatc gcgccgatgg ttgccagata gccgttaccg cggcccattt
    15121 gacgacctac tgcggcgacc cgacgctcca gacgaaagct ggtacgttct ttaatacctt
    15181 cgttatcgtc gctgccttct gacagttcca gctcattctg cgcttcattg agcaaatgca
    15241 ggcttaagct tttgctacca aaatcagcgg cgatatcgtt ggcctggttt aaggaacgcg
    15301 cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt ctgattgaag aactctacgc
    15361 tcttactgaa gaagattgcc caggtgacta cggaggccaa aataagccca atcatcacgc
    15421 acttaacgac aatatcggcg tgctgataca taccccagac ggaaaggtcc gtctgcatta
    15481 aattattacc cactgtgtat ctccaggacg caagtcacaa aatctgcgca taataatatc
    15541 aaaacgacgt cgaattgata gtcgttctca ttactatttg catactgccg tacctttgct
    15601 ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca cgccatttgc gaaaattttc
    15661 tgctttatgc caattcttca ggatgcgccc gcgaatattc atgctagttt agacatccag
    15721 acgtataaaa acaggaatcc cgacatggcg gacaaaaagc ttgatactca actggtgaat
    15781 gcaggacgca gcaaaaaata cactctcggc gcggtaaata gcgtgattca gcgcgcttct
    15841 tcgctggtat ttgagagtat ggaagccaaa aagcacgcga cgcgcaatcg cgccaatggc
    15901 gagttgttct atggacggcg cggaacgtta acccattttt ccttacaaca agcgatgtgt
    15961 gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg gggcggcagc ggttgctaat
    16021 tcgattcttg cttttgtcga acagggcgat catgtgttga tgaccaacac cgcctatgaa
    16081 ccgagtcagg atttctgtag caaaatcctc agcaaactgg gcgtaacgac atcatggttt
    16141 gatccgctga ttggtgccga tatcgttaag catctgcagc caaacactaa aatcgtattt
    16201 ctggaatcgc cgggttccat cactatggaa gtccacgacg ttccggcgat tgttgccgcc
    16261 gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca acacctgggc agccggtgtg
    16321 ctgtttaagg cgctggattt tggcatcgat gtttctattc aggccgccac caaatatctg
    16381 gttgggcatt cagatgcgat gattggcact gcggtgtgca atgcccgttg ctgggagcag
    16441 ctacgggaaa atgcctatct gatgggccag atggtcgatg ccgataccgc ctatataacc
    16501 agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac atcatgaaag cagtctgaaa
    16561 gtggctgaat ggctggcaga acatccgcaa gttgcgcgag ttaaccaccc tgccctgcct
    16621 ggcagtaaag gacacgaatt ctggaaacga gactttacag gcagcagcgg gctattttcc
    16681 tttgtgctta agaaaaaact caatgatgaa gagctggcga actatctgga taacttcagt
    16741 ttattcagca tggcctactc gtggggcggg tatgaatcgt tgatcctggc aaatcaacca
    16801 gaacatatcg ccgccattcg cccacaaggc gagatcgatt ttagcgggac cttgattcgc
    16861 ctgcatattg gtctggaaga tgtcgacgat ctgattgccg atctggacgc cggttttgcg
    16921 cggattgtat aacattgcca cttttggaca attttgcaga cattttattg tgaaaagtct
    16981 taaattgttg cgtccgggat caaggcgtcc cggacgattc aggagtacaa taggcagata
    17041 aaggcttaaa cgctgttcca caggaaagtc catggctgtt attcaagata tcatcgctgc
    17101 gctctggcaa cacgactttg ccgcgctggc ggatcctcat attgttagcg ttgtttactt
    17161 tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg cccgcctcat ttttgccagg
    17221 cgacagcttg ttgatattgg ctggcgcatt gattgcccag ggggttatgg attttctgcc
    17281 tacgattgcg attctgaccg ccgcagcaag tctgggctgc tggctaagtt atattcaggg
    17341 gcgctggtta gggaatacca aaacggtgaa aggctggctg gcacagcttc ctgctaaata
    17401 tcatcagcgc gccacctgca tgtttgaccg ccacggtctg ctggcgctgc tggctggacg
    17461 ttttcttgca tttgtccgta cgctgctgcc aaccatggcg ggaatttccg gtctgccaaa
    17521 ccgccgtttc cagtttttca actggttaag cggattgctg tgggtcagcg tggtaaccag
    17581 tttcggctat gccttaagta tgattccgtt cgttaaacgc catgaagatc aggtaatgac
    17641 gttcctgatg atcctgccaa ttgccttgtt aaccgctggc ttgttaggca cgctgtttgt
    17701 ggtgattaaa aaaaaatact gtaacgcctg acgattttcc ctgttcccgg ttgctgtacc
    17761 gggaacgtat ttaattcccc tgcatcgccc gcattcttgc cgcatcttcc cccggcgtca
    17821 caccgaagta acgtttaaac tcacggctaa attgcgatgc gctttcatag ccgacgcgca
    17881 tcgctgctgc gctggccttc atgccgtcat ggatgatcat catccgcgcc ttatgcagac
    17941 ggtaattctt caaatactgc aacggcgagg tgctggtgac agacttaaaa ttatggtgga
    18001 acgccgatac gctcatattg gcttctgccg ccagttgctc gacgctcagg ttttcggtgt
    18061 atttattctc aatccgtttc agcacgcggc taatcagact gaagtgagtc tggcgactga
    18121 ccagcgccag taacgcgccg ccgcaaggtc cggttagcac gtagtacaga atttcgcgga
    18181 tgatctgttt gccgagaata cgcgcatcca gtggtcgctc catcacgtcg agtaaccgct
    18241 ccgccgcgca taaaatctct tctgataacg tggcggagtt aatcccgctg gctgccatcg
    18301 acggctggaa atgctcatct tcgccaatgt ccatcaacag ttcctgtaac tgcaaaatat
    18361 cgacattgag acgcaaccct gccagcggca cctctgacgt agcataggtt tcgcactcaa
    18421 acggcaacgg caccgtcagc agcaggtatt cattggcatc ataacgaaac acgcgttcat
    18481 tgatataacc gattttatgt ccggaaaaga gaattatgat gccaggctcg tacatcaccg
    18541 gtgtacgtgc gaaaggcgtc tcgccataca acaaacgcac atcgggcaac agttctgaca
    18601 aactattttc tttatttttc aggttattaa ctttattcgc cagcaagcgg caaatctctt
    18661 cacgtttcat atcgcgtaat ttcttaggaa taatgcggca atttgattgt gcgcaatttt
    18721 gtagcatttc tccagcactc tggagaaata ggcaagacat tggcagaaat gagcattgag
    18781 agccagggcg ctggcgatca caatgaaaaa catcaggcag atcgttctct gccctcatat
    18841 tggcccagca aagggagcaa gtaatgaaca actttaatct gcacacccca acccgcattc
    18901 tgtttggtaa aggcgcaatc gctggtttac gcgaacaaat tcctcacgat gctcgcgtat
    18961 tgattaccta cggtggcggc agcgtgaaaa aaaccggcgt tctcgatcaa gttctggatg
    19021 ccctgaaagg catggacgtg ctggaatttg gcggtattga gccaaacccg gcttatgaaa
    19081 cgctgatgaa cgccgtgaaa ctggttcgcg aacagaaagt gactttcctg ctggcggttg
    19141 gcggcggttc tgtactggac ggcaccaaat ttatcgccgc agcggctaac tatccggaaa
    19201 atatcgatcc gtggcacatt ctgcaaacgg gcggtaaaga gattaaaagc gccatcccga
    19261 tgggctgtgt gctgacgctg ccagcaaccg gttcagaatc caacgccggt gcggtgatct
    19321 cccgtaaaac cacaggcgat aagcaggcgt tccattcagc ccatgttcag ccggtatttg
    19381 ccgtgctcga tccggtttat acctacaccc tgccgccgcg tcaggtggct aacggcgtag
    19441 tggacgcctt tgtacacacc gtggaacagt atgttaccaa accggttgat gccaaaattc
    19501 aggaccgttt cgcagaaggc attttgctga cgctgatcga agatggtccg aaagccctga
    19561 aagagccaga aaactacgat gtgcgcgcca acgtcatgtg ggcggcgact caggcgctga
    19621 acggtttgat tggcgctggc gtaccgcagg actgggcaac gcatatgctg ggccacgaac
    19681 tgactgcgat gcacggtctg gatcacgcgc aaacactggc tatcgtcctg cctgcactgt
    19741 ggaatgaaaa acgcgatacc aagcgcgcta agctgctgca atatgctgaa cgcgtctgga
    19801 acatcactga aggttccgac gatgagcgta ttgacgccgc gattgccgca acccgcaatt
    19861 tctttgagca attaggcgtg ccgacccacc tctccgacta cggtctggac ggcagctcca
    19921 tcccggcttt gctgaaaaaa ctggaagagc acggcatgac ccaactgggc gaaaatcatg
    19981 acattacgct ggatgtcagc cgccgtatat acgaagccgc ccgctaagct ttttacgcct
    20041 caaactttcg ttttcgggca tttcgtccag acttaagttc acaacacctc accggagcct
    20101 gctccggtga gttcatataa aggaggaacg tatggctaat ccaaccgtta ttaagctaca
    20161 ggatggcaat gtcatgcccc agttgggact gggcgtctgg caagcaagta atgaggaagt
    20221 aatcaccgcc attcaaaaag cgttagaagt gggttatcgc tcgattgata ccgccgcggc
    20281 ctataagaac gaagaaggtg tcggcaaagc cctgaaaaat gcctcagtca acagagaaga
    20341 actgttcatc accactaagc tgtggaacga cgaccacaag cgcccccgcg aagccctgct
    20401 cgacagcctg aaaaaactac agcttgatta tatcgacctc tacttaatgc actggcccgt
    20461 tcccgctatc gaccattatg tcgaagcatg gaaaggcatg atcgaattgc aaaaagaggg
    20521 attaatcaaa agcatcggcg tgtgcaactt ccagatccat cacctgcaac gcctgattga
    20581 tgaaactggc gtgacgcctg ttattaatca gatcgaactt cacccgctga tgcaacaacg
    20641 ccagctacac gcctggaacg cgacacacaa aatccagacc gaatcctgga gcccattagc
    20701 gcaaggaggg aaaggcgttt tcgatcagaa agtcattcgc gatctggcag ataaatacgg
    20761 caaaaccccg gcgcagattg ttatccgctg gcatctggat agcggcctgg tggtgatccc
    20821 gaaatcggtc acaccttcac gcattgccga aaactttgat gtctgggatt tccgtctcga
    20881 caaagacgaa ctcggcgaaa ttgcaaaact cgatcagggc aagcgtctcg gtcccgatcc
    20941 tgaccagttc ggcggctaac gtgcaaattc tcccggtggc ggtaatgttc cgctaccgga
    21001 cttttcagaa atcatttatt cccctcgcgt cccgcccgtt gttactcttc cttgttcagg
    21061 aatgccaaat ataaggacat catcatgcag agccggaagc tcttaaaaga acaactcatc
    21121 tatatccggg ataaacgcaa cggagaggtg aaaaacagat gaaaataata cttctgtttt
    21181 tagcagccct ggcaagtttt accgtgcacg cacagccccc ctcacagacc gtagaacaaa
    21241 cagtccggca tatttatcag aactataaat cagatgccac tgccccttat tttggtgaaa
    21301 ccggagagcg ggcgataact tctgcgcgta ttcaacaggc gcttaccctg aacgacaatc
    21361 ttacgctgcc gggcaatatt ggctggctgg attatgatcc ggtttgtgat tgtcaggatt
    21421 ttggcgatct ggtgctagaa agcgtagcga taaccgaaac tgacgccgat catgccgatg
    21481 ccgttgtgcg ctttcgtatc tttaaagatg ataaagaaaa gaccacgcag acactgaaaa
    21541 tggtggcgga aaatggtcgt tgggtcattg acgatattgt cagcaatcat ggcagcgtct
    21601 tacaagcagt taatagcgag aatgaaaaaa cgctggccgc tttagcttcg ttgcaaaaag
    21661 aacagccgga agcctttatt gccgaactct ttgaacatat tgccgattat agctggccgt
    21721 ggacgtgggt ggtttccgac tcttaccgcc aggcggtcaa tgccttctat aaaaccacct
    21781 tcaagacggc caacaatccc gatgaagata tgcaaataga acggcaattt atttacgaca
    21841 atccgatctg ttttggcgaa gagtcgctat tttcacgcat tgatgaaatt cgagtcctgg
    21901 agaaaaccgc cgattccgcc cgcattcata ttcgttttac gctgaccaat ggcaacaacg
    21961 aagagcaaga actggtttta caacggcgcg aaggtaagtg ggaaatcgct gattttatcc
    22021 gcccgaacag cggcagccta cttaagcaga ttgaggcaaa aaccgccgcc agattaaagc
    22081 aatgagctga attaaataac aattagccgg aacaataaat aaaagggaac actatatgaa
    22141 aacgattttc accgtgggag ctgttgttct ggcaacctgc ttgctcagtg gctgcgtcaa
    22201 tgagcaaaag gtcaatcaac tggcgagcaa tgtacaaaca ttaaatgcca aaatcgcccg
    22261 gcttgagcag gatatgaaag cactacgccc acaaatctat gctgccaaat ccgaagctaa
    22321 cagagccaat acgcgtcttg atgcacagga ctattttgat tgcctgcgct gcttgcgtat
    22381 gtacgcagaa tgataaaaaa atccccggca gcatgtcagt tgccggggat tttttttaac
    22441 gtccaaccgc cgctttaggg cgtttcttcg caccagtatt caccggacga gattgcatag
    22501 acggcgcttt ttttgccgta gcaggagtct gacgctgggt cgccatcggc gtatgtttcg
    22561 tcaacgctgg acgagtattg cggttctgac gacgagcctc acgcatctct tcaatggttg
    22621 gcgcaggcac taagcaatcg cgacggctac caatcagatg ctttttaccc attgcttcca
    22681 gcgcctgacg gattaacggc cagtttgccg gatcgtggta acgcaacaac gctttatgca
    22741 aacgacgctg tttgtcgccc ttcggtacga agacgtcttc actcttataa ccaatcttcg
    22801 ccagcgggtt tttcccggtg taatacatgg tggtcgagtt agccagtggc gatgggtaga
    22861 agttctgtac ctggtcgaga cggaaacgat gctttttcag ccacagcgcc agattcacca
    22921 tatcttcatc acgcgtaccc gggtgcgcgg agatgaaata ggggatcaga tactgctctt
    22981 tacctgcctg tttcgagtag gtatcgaaca gctctttaaa gcggtcataa ctgcccatgc
    23041 ccggcttcat catcttcgac aacggccctt cttcggtatg ttccggagca atcttcagat
    23101 aaccgccgac gtgatgggtc gccagttctt tgatatagcg cggatcttct acggcgatgt
    23161 cataacgtac gcctgaggca atcaggatct ttttaatgcc tttcagatcg cgagcgcggc
    23221 gatagaggtt gatcgtcggt tcgtggttag tgtccatatg cggacaaata tccgggtaaa
    23281 cgcaggacaa acgacggcag gtttgttcag cgcgtggcga tttgcagcgc aacatataca
    23341 tgttggcagt tggcccaccg agatcggaaa ttacgcccgt aaaacctgga acggtgtcgc
    23401 ggatcgcttc gatctcatta atgatcgaat cttcggaacg gctctgaata atgcgccctt
    23461 cgtgctcggt gatagaacag aaagagcagc cgccaaagca gccgcgcata atgttgaccg
    23521 aaaaacggat catttcgtaa gccggaatac gggcattgcc ataggccgga tgtggcacgc
    23581 gcttgtatgg cagcgcaaaa acgctgtcca tctcttcggt agaaagcgga atagcaggcg
    23641 ggttgatcca cacatagcgg tcgccgtgtt tttgcatcaa tgcgcgggcg caacctgggt
    23701 tagtttcgtg gtgcagaatg cgcgaagcat gagcgtacag gactttatcg cccttcactt
    23761 tctcgaaaga aggcagcaat acgtaggttt tttcccacgg tttcgggcgc ggcggctgca
    23821 cagttacagc tttcgcttcc tgctttttcg gcgcaacagg tttgttatcc gcacacggca
    23881 aatcttcgcc atacggatgc gggatcgggt cgattttccc aggggtatca agacgagtag
    23941 aatccacacc gctccagcct ggcaacgcct cttttacgat aatcgcggta ttacgcacat
    24001 cgcggatttc actaatcggc tcgcccatcg ccagacggtg cgccacctcc accagcggac
    24061 gctcaccgtt accaaacatc agcatgtcgg ctttcgaatc caccagcacg gaacggcgca
    24121 cggtatcgga ccagtaatca taatgcgcgg tacggcgcag actagcctca ataccaccga
    24181 ggatcaccgg tacatctttc cacgcctctt tacaacgctg ggtataaacc agtgtggcgc
    24241 gatccgggcg cttacccgcg acgttatccg gcgtgtaggc atcgtcatga cgtaaacggc
    24301 gatcggcggt ataacggttg atcatcgaat ccatgttgcc agcagtaaca ccgaaaaaca
    24361 gattcggttt acccagacgc ataaagtcgt ctttgctgct ccagtctggc tgggcgatga
    24421 tcccgacgcg aaagccctgt gcttccagca tacgaccgca aatcgccatc ccgaagcttg
    24481 gatgatcgac atacgcgtcg ccagtaacca aaatgatgtc gcagctatcc cagccaagtt
    24541 gatccatctc ttcacgagac atcggcaaaa acggtgccgg tccaaaacag gcggcccagt
    24601 actgcggcca ggagaacagg tcgcgatccg gttggatcag ggagatagag ctcattttgc
    24661 ttccagaaat gataaaaaaa taatcaaagg ccggggatta taagccggaa cgaaagagaa
    24721 atcgaaaagt attccatact cgccctcctc gggcgagtat gaagattacg gtaccggatt
    24781 gactaacagt tgcccaatcg agccacggtc cgccatttcc agcgtctgac tgttgaagta
    24841 gaacgggaag tgcgcccagg aaggctgacc gaaataaaca agcagctcca cttgtccgtc
    24901 aacccaaacg gtatctttcc agcctctgtc ttccgggaac ggcatcgcac cattcacgtt
    24961 acggatctgg aacatcacgc cttcaatatg gaacgcttgc ggctcgtccg cgcgtaccgt
    25021 ccagcgttcc cacgttcctt gctgcgcggt gacatcaata cggttgacgt cccacagttg
    25081 tccattaata cccgggtcat cacccagact gatatcgcga ctgcgaatcg gcgaaccggc
    25141 catgatttca gttggcagca agcgcatcgg aagactgtct gtgaccagcg gcagaaggcc
    25201 ggttgggcgc agcgttagca ccagggtaga aaccagaatg ctggatggct caaagaagcc
    25261 acgaatacga tcaacaatgc tcgccgcttc gccacaggtg atcgacactt catcaccgtt
    25321 gctcatatcc accagaatct cgcggcgctc gcccggtgcc aacgaaagtt gcttcaccga
    25381 tacaggagca ggcaggaatc cctgatcgcc agaaatcaca tgtaacgggc gaccatcgct
    25441 catctgtagt tgatagcgac gagagttcga cgcgttcagc agtcgcaagc gcacccagcc
    25501 acgcgagact tcaacgtacg ggctttgtac accgttaacc agcagcgtat caccaacaaa
    25561 gccgccgctt cccggttcgt tgtattctgg cgtaccaaag ttatccagcc gtttatcctg
    25621 gataatgacc ggaaaatcat ccacaccata atggttgggg ataggcagcg acttgctgac
    25681 ttcatcttcc accagccaca ttccggcaag gccgttatag acctgctgag ccgtgcggtt
    25741 gggagtattg gcgtgatacc acagagtagc tgcgttctgg cgaataggca gtacgggtgc
    25801 ccagtcagcg tttggcgaca tcatccgtgc cggaccgccc atcagcgggc ctggtacctg
    25861 tagcccggcc accgtcattg agacattttc tgtcaggcgg ttgctgtaaa taagcttaac
    25921 atcgtcgccc ttccagacgc ggatagtcgg ccccaggtaa cgaccattga ttccccagac
    25981 cgacgcgcgt gtccctggcg taaatgacca gtgcgcacgt tgtacagtca taaacagcgg
    26041 ttgcccacgg cgagattcaa gcagcggcgg aacgggtagc ggttgctgtt gcccggctgc
    26101 gctggccttc aggggaacag cgcctgcaca aagtgcaatc cccgatgcct gaatgaactg
    26161 acgccgactg agtgacatat ttgctccatg taaaactggc taaagtaata ccgtgcagga
    26221 aagtgttccc cgcaaaaata cagattgccg acttaaactt ttccggcggc ttcgcgttct
    26281 gcgacttctt tatcgagctc ggcgattttt tgttccatta tcgaacgaca atgggcagcc
    26341 agctcacgaa cctgatcttt gccatactga ctgacgtcaa ttggcggcag catttcgaca
    26401 atcaccagac cgttgtgcag tcgattaaga ttaatcttat tcgaagttgt agagacgcac
    26461 acgggaataa tcgggacgcc tgctgcaatt gccgcgtgaa atgccccggt cttgaacggt
    26521 agcaggccgc gaccacggct gcgggttcct tccgggaaca tccaaatgga aatacggcgt
    26581 tttttgaaat gattcactac ttctgcaata gtgccgtgag ctttagtgcg attgtttctg
    26641 tcgatcaata agttgccggt taaccagtac aactgcccga agaaggggat ccacagcaag
    26701 ctctttttac ctaccgtcac cgtcggcggt tgcacaatgt tcgatgctgt caccatgtca
    26761 tagttgttct ggtggttagc gatatagata gcattgccgt agctttcagc atctgcaggt
    26821 ttgcggcact caactttcag gccgaacagc ggcgcaagac gaccaaacat atgcccaaag
    26881 gtggccacat gtttcgggtt acgcgggctg aaaaggcagt aaatggagcc gaatacacag
    26941 actaagatgc tgtaaatcac ggtaataata agacgaaaga tatatagcat aacaccctct
    27001 gaaggtctga gagactggca ttgtacgtca cctgaaatca ggttagggca ttgttaaaag
    27061 ccgctctctg gaaaagcgta ttgttaatcg caacgcatga ataaacaacg gtttcaaggg
    27121 aatttttatc gtttccgggg gttgaatacg ctgccggatg acgacttaac gtttcatccg
    27181 gcgttccttg caagcgggag gaaacagcgc cctccccggc atattactct tcgctatcac
    27241 cgctgctggc acggcgagga gagtcgatct caacacgatc gatacgctgc aaaccgcgca
    27301 tcaacgtacc gcggcgtcca cgttcgccag tgactttctg tagctcttcc ggacgcagtt
    27361 taattttgcg tttcccaaca tgaatggtca gcgtgctttg cggcggcaga acgtacagtt
    27421 gcgccagacc atcctcgcca cgcgcggctt ctgccgatgg aatgttgata atcttgttgc
    27481 ctttgccctt cgacagctgc ggcagatcac ttaccgggaa catcaacata cggcctgcct
    27541 gagtgattgc cagcagcatg tcggaagcat cttcaatcac caccggcggc ataacatggg
    27601 cattttccgg taaggttatc aaagccttac ctgcacggtt acgcgccacc agatcgttaa
    27661 aggtgcagac gaaaccgtaa cccgcatcgg aagccatcag cagtttctga tcgtcgcttt
    27721 ccatcagcat atggtcaacg gtcgccccag gcggcaacgt taatttgccg gtgagcggtt
    27781 cgccctgacc acgcgccgac ggcagcgtaa tcgggtcaat ggcatagcta cgaccggtgg
    27841 aatcaacaaa cactaccggt tggttgctct tacctttcac cgccgctttg aagctatcac
    27901 ccgctttata attcaggccc ggcgcgtcga tatcatggcc tttagcgctg cgtacccagc
    27961 ccatctgcga cagcacaatg gtgacaggtt cagacggcag catgtcgtgc tcgctcatcg
    28021 ctttcgcttc ttcgcgttcc tgcaacggcg aacgacgatc gtcaccgtag gcttgcgcgt
    28081 ctgcctgcag ttctttcttc agcaggttat tcattttacg ctcggaagcc aaaatgccct
    28141 gcaactggtc gcgctctttt tccagctcac tctgctcacc gcgaatcttc atctcttcca
    28201 gtttggcaag atgacgcagt ttcagttcga ggatcgcttc cgcctgggtt tccgtaaggc
    28261 caaaccgcga catcagcgcc ggtttcggtt catcttcatt acgaatgatc tcaatcactt
    28321 cgtcgatatt gagaaacgcc accagcaaac cttcgaggat atgcaggcgc ttgaggactt
    28381 tctccagacg atagttcagt cggcggcgca cggtatcgcg gcggaacacc agccattcgg
    28441 agaggatttc cagcaggttt ttcaccgccg gacgaccatc cagaccgatc atattgaggt
    28501 taatgcgata gctcttttcc agatcggtgg tagcgaagag gtggttcatc acctgatcca
    28561 tatccacgcg gttggaacgc ggcacaatca ccagacgggt cgggttctcg tggtcagatt
    28621 catcgcgcag atcgtcaacc atcggcagct ttttgttgcg catttgcgca gcaatttgct
    28681 ccagtacgcg cgcacctgaa acctgatgcg gcaatgcgct gataaccacc gcgccatctt
    28741 ctttcttcca caccgcgcgc atacgcactg aaccacgtcc gttctcgtag attttacgga
    28801 tctcggcgcg cgaagtgata atttccgctt cagtcggata atccggcccc tgcacgatat
    28861 ccagcagctg atcgagcgtg gttttcggct ggtcgattaa tgcgattgcc gcctgagcca
    28921 cttcacgcag gttatgcggt ggaatatcgg tcgccatgcc gacggcaatt ccggtggtgc
    28981 cgttaagcaa aatgtttggc agacgggcag gtagcatttt cggctcctgc aaagtgccgt
    29041 cgaagtttgg cacccagtca gccgtcccct gccccaactc gctcaatagc agctcggaat
    29101 atttcgacaa ccgggattcg gtgtaacgca ttgccgcgaa cgatttcgga tcgtccggcg
    29161 cgccccagtt cccctgacca tcaaccagcg gataacggta agagaacggc tgcgccatca
    29221 ggaccatcgc ttcataacag gcgctatcgc cgtgcggatg gtatttaccc agtacgtcac
    29281 cgacggtacg ggccgatttt ttaaatttgg cgctggcatt caggcccagt tcagacatcg
    29341 catacacaat gcggcgctga acaggtttca gaccatcacc aataaacggc aacgcacggt
    29401 ccatgatcac gtacatggag tagtttaagt aggcgttttc cgtaaattca tgtagcgcaa
    29461 ggcgctctgc catatcgctc attaattctg attcctcaac ttattcgcca gccttcaaag
    29521 gggcaatatt gccggagata ctacattatc tgccgcgtcg agtcacagga gatcagcgtg
    29581 aatagcgccg ccggacgtgc tccgacggcg ataaaggcat caatgtgcct tgatatacaa
    29641 ctctttgctg tagacataat cctggggatt atgtagcgga aaaccgccaa cgtagggttt
    29701 aagcagtttg attaacggct gatagtagat aggaatcagc ggtgcctgtt ggttgatgat
    29761 cacttctgcc tgctgataca acgcattacg ctttgtcgca tcagtgatct gcgtggcctg
    29821 gtttagtaag gcgtcatact gcgcattttt ccagtgaccg acgttttctt cactatcgct
    29881 tttgagcgtg ttcaggaagc tggaagcatt attgtacgtc gcatcccacg actgccgaga
    29941 cagcatgaaa tcaccggctc gtcgggcatc aagataggtt ttccactcca ttgtgcgcag
    30001 cgtcacctgt gcacccagcc attttttcca ttcggaagac aacgctatcg cggtcttttc
    30061 atgcagatcg tacttgttgt agaacagctc aaagcgtagc ggatgagagg cgtcgtatcc
    30121 cgcctgcttc agcaaggctt ttgccatcgc gacgcgctca ctcattggct tttgcagttc
    30181 atcgaacgtc gtcacgctaa agccttttac ctctggcggc gtcagcgtgg ttgcgggcgt
    30241 cctcaacccc agtacctttt gcgcaataag ctgtcgatca accgtaagat atagcgcccg
    30301 acgcactcgc acatcgttaa atggcggttt ctcaaggttg aagttgtaat attcgctgtt
    30361 cagacgcgga ataattcgta gctcgccagg cagtgatttt tcaatggcgg gaatttgctg
    30421 cgccggaacc caggtgagat cgacctctcc cgcgcgatag cggttatagc cggtgaccga
    30481 attatccagc gccagatact caacctgttg caatactgta tgttgcgcat cgcggtactt
    30541 tggatttttg cgtgcagtaa tcttttcgtt aactacccac tgatcaagca caaaggcacc
    30601 gttgtaaacc atgttctctg gcttactcca gctatcgcca tgtttagcga tgacatgatg
    30661 aggaaccggg aacagcgtcg gccaggcgag catcgtcgtg aaccaaggaa ccggctgctc
    30721 aagcgtaact tcaagagtac gatcatccgt cgctttgaca cccagcgatg taacatctgc
    30781 tttacccgca acaatagccg cggcattgtt aatgtgcgcc tgtgccagat agccagcaaa
    30841 agggcttgcc gttttcgggt caaccgcgcg ctgccagccg aggacaaaat cctctgccgt
    30901 cagaggctga ccgtcggacc actgcaaacc gctacgcaga tggaaaatat agcgcctgcc
    30961 gccgtccatt atctcccagc gttcagcctg agcgggctgc acctggcctt caccgtccat
    31021 ccataccaga ccttcaaaca gatccagcac aatctgcgca gcagtattct cctccacctt
    31081 ttgcgggtcg agcgtacctg ggtcgctatg attgttgtaa cgaaagactt gttgcggggc
    31141 gagctgtgtg ttggcgggaa cgtcagcagc gtagagagga gccgcactta ccaggctgac
    31201 cagccataat aaatttcgcg tatacatggt attccttacc ggagaaatta taagcccaac
    31261 aacaatacgc ctgtggcatt gttcgctcaa acttatcgcg agtgatttgg ctcacactcc
    31321 ggtaaagatt ccccatcacg ctaacggcac ataaacatcc gtttccagtc gctgctctgt
    31381 caccccttcg gcaagattgg tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc
    31441 gcttgcaggc agccagtggc gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc
    31501 gccaacgtgg cgggccacgg cataacgtcc accggtaagt tcaccattgc tcacaccata
    31561 acgattatcg ggaatcggtt cgctaacgct gccgcagata tcaaagcgaa acgcttccgg
    31621 tgcggtggtg gcagggtcgt cccaggcgac gccaaaagtc tggctttggt taacaggaga
    31681 aagccccgtc tctttacgcc acatgataaa tttcgccaca ctgtagttga gcaactcagg
    31741 gctacagcgg tgcggcaaca tcgccacagg gattgatggg aaatcgataa tgtttacatc
    31801 cagtgtcagg tttgtcattt tgcctccctg cgaaattatt cccgggaacg cgtcccggga
    31861 gcggtaacaa ttacggattt actttgcgga tctgtttgac gtcaatttca acagaattcc
    31921 agtctttatc gacttcaccc tgaatctcaa ccgtatcttt cggcgtcacc gtcacgccgt
    31981 tccagcgttt gtgatcgata tcaacattga tagtaccgct ggcatctttg aacacgtaga
    32041 gatcgtcaga gatgcgttca acgatattgc cgcgcagggt tacccaggtg tcgtcacgca
    32101 gggattttgc gctttctaca gtcgttacgc tgccgttcgg cccctggaat cctccggcct
    32161 gactttgcgt tgccgatggg ccagaaaaac cgccctgctc tgctgccatc accggtgcgc
    32221 tgcacagggc cattactgcg attactgctg cgaatttttt catgtttatt actcccttta
    32281 atgtctgttt ccgag
//
LOCUS       DAZUCE010000028        32016 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZUCE010000028 DAZUCE010000000
VERSION     DAZUCE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.028"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(72..1832)
                     /gene="ycaO"
                     /locus_tag="ACHY03_001160"
     CDS             complement(72..1832)
                     /gene="ycaO"
                     /locus_tag="ACHY03_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFS3904419.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRDTILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1962..2654
                     /locus_tag="ACHY03_001161"
     CDS             1962..2654
                     /locus_tag="ACHY03_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFS3904420.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2853..3941
                     /gene="serC"
                     /locus_tag="ACHY03_001162"
     CDS             2853..3941
                     /gene="serC"
                     /locus_tag="ACHY03_001162"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFS3904421.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4012..5295
                     /gene="aroA"
                     /locus_tag="ACHY03_001163"
     CDS             4012..5295
                     /gene="aroA"
                     /locus_tag="ACHY03_001163"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFS3904422.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALAALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEVENQ
                     HYQQFVVKGGQPYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5464..6228
                     /gene="ycaL"
                     /locus_tag="ACHY03_001164"
     CDS             5464..6228
                     /gene="ycaL"
                     /locus_tag="ACHY03_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFS3904423.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6401..7084
                     /gene="cmk"
                     /locus_tag="ACHY03_001165"
     CDS             6401..7084
                     /gene="cmk"
                     /locus_tag="ACHY03_001165"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFS3904424.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7195..8868
                     /gene="rpsA"
                     /locus_tag="ACHY03_001166"
     CDS             7195..8868
                     /gene="rpsA"
                     /locus_tag="ACHY03_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFS3904425.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9028..9312
                     /gene="ihfB"
                     /locus_tag="ACHY03_001167"
     CDS             9028..9312
                     /gene="ihfB"
                     /locus_tag="ACHY03_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFS3904426.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9519..11783
                     /locus_tag="ACHY03_001168"
     CDS             9519..11783
                     /locus_tag="ACHY03_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFS3904427.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTLRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIALHQPL
                     TGRFLQASVIEPSCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCATFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNGILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11820..13568
                     /gene="msbA"
                     /locus_tag="ACHY03_001169"
     CDS             11820..13568
                     /gene="msbA"
                     /locus_tag="ACHY03_001169"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFS3904428.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     LERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13565..14551
                     /gene="lpxK"
                     /locus_tag="ACHY03_001170"
     CDS             13565..14551
                     /gene="lpxK"
                     /locus_tag="ACHY03_001170"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFS3904429.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEKLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14588..15820
                     /gene="ycaQ"
                     /locus_tag="ACHY03_001171"
     CDS             14588..15820
                     /gene="ycaQ"
                     /locus_tag="ACHY03_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFS3904430.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15872..16054
                     /gene="ycaR"
                     /locus_tag="ACHY03_001172"
     CDS             15872..16054
                     /gene="ycaR"
                     /locus_tag="ACHY03_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFS3904431.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16051..16797
                     /gene="kdsB"
                     /locus_tag="ACHY03_001173"
     CDS             16051..16797
                     /gene="kdsB"
                     /locus_tag="ACHY03_001173"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFS3904432.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16951..17844
                     /locus_tag="ACHY03_001174"
     CDS             16951..17844
                     /locus_tag="ACHY03_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFS3904433.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17821..18600)
                     /gene="elyC"
                     /locus_tag="ACHY03_001175"
     CDS             complement(17821..18600)
                     /gene="elyC"
                     /locus_tag="ACHY03_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFS3904434.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18736..19521
                     /gene="cmoM"
                     /locus_tag="ACHY03_001176"
     CDS             18736..19521
                     /gene="cmoM"
                     /locus_tag="ACHY03_001176"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFS3904435.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLHPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19518..20840
                     /gene="mukF"
                     /locus_tag="ACHY03_001177"
     CDS             19518..20840
                     /gene="mukF"
                     /locus_tag="ACHY03_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFS3904436.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20821..21525
                     /gene="mukE"
                     /locus_tag="ACHY03_001178"
     CDS             20821..21525
                     /gene="mukE"
                     /locus_tag="ACHY03_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFS3904437.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            21525..25985
                     /gene="mukB"
                     /locus_tag="ACHY03_001179"
     CDS             21525..25985
                     /gene="mukB"
                     /locus_tag="ACHY03_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFS3904438.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26246..28093
                     /gene="ldtD"
                     /locus_tag="ACHY03_001180"
     CDS             26246..28093
                     /gene="ldtD"
                     /locus_tag="ACHY03_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS3904439.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEIIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28274..28822
                     /locus_tag="ACHY03_001181"
     CDS             28274..28822
                     /locus_tag="ACHY03_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFS3904440.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28849..29496
                     /gene="gloC"
                     /locus_tag="ACHY03_001182"
     CDS             28849..29496
                     /gene="gloC"
                     /locus_tag="ACHY03_001182"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFS3904441.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29547..30737)
                     /gene="aspC"
                     /locus_tag="ACHY03_001183"
     CDS             complement(29547..30737)
                     /gene="aspC"
                     /locus_tag="ACHY03_001183"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFS3904442.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30922..31995)
                     /gene="ompF"
                     /locus_tag="ACHY03_001184"
     CDS             complement(30922..31995)
                     /gene="ompF"
                     /locus_tag="ACHY03_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFS3904443.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNK
                     TRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAAD
                     RTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPIEGGFANKTQDVLL
                     VAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQ
                     IDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7768 a         7830 c         8651 g         7767 t
ORIGIN      
        1 ggcattaaaa aaacgccccg taatataact cagacgaatc attaagccta cattgcgtag
       61 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
      121 ttcaacagcg actgatgtgc agcaaacgcg tgcagatcgc tatctactgg ttgcaagcca
      181 taaaacgccg cttcgccgct cattgccgca ctggcggctt ccacggcatc tgcgccgtac
      241 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
      301 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg ggctgaatac cgatgagtta
      361 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
      421 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
      481 agacccaaca gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg
      541 ataaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgagag aatggtgtcg
      601 cgcagatgac tacccatact gttattcgct aaccacaaat cttcagccgg ataaatatcg
      661 gacatgccag gcacgataat acggcaagca taaacgccca gatgctcgta atcggcaata
      721 taaacttctt tatcttcttt gttgaagata gccatcagcg tggcgaactc ttcctccgtg
      781 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
      841 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
      901 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
      961 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     1021 aagcaggtgc cgttagcagg attaaacagt accacgcaaa tcaccggata ctggccgcca
     1081 agggaaccat catatgcgaa gatcgggaaa ccttctgctt ccagtgtttc gatcgcttca
     1141 accaccgctg ggtaacgcgc cagcacgtct gccgggattt ccggcaagct gatgctttca
     1201 gcaataatgc ggtttttcac gtagcgttcg aaaacttcgg acaacccctg aacgcgtgct
     1261 tcgttgcggg tattgccagc ggacataccg ttagaaacgt acaggttacc aatgatattc
     1321 atcggaatat aaacggtctg attgtcggac tggcgcgtaa acggcagacc gcaaatacca
     1381 cgatcttcgt taccggattg taggtcaatc agcatactgc cggtcaattc attctccgga
     1441 tcgtaaaatg cgcgcagacg gtcatcgagc agcccttctg gcacatcgtc attttcggtc
     1501 agtgggaacc atttttcgtt gggataatgc acaaatggac cgttggcgat ggtttcgccc
     1561 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accgagtgca
     1621 gaagccagcg ccgctttctt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     1681 ttgtcgcgaa tatgtacaga ccagacgtta ggcacgggat tcagccagga ggcctcttca
     1741 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     1801 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     1861 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     1921 gactatgctt ttagcggata aaccacgtta agagcataaa aatgaaggcg ttcgatctcc
     1981 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     2041 tgtatacctt tgtactggtt tttttgttcc tcaaaatgac cggaagacgc ggagtgcggc
     2101 agatgtcgct gtttgaagtt ttaatcattc tgacgctggg gtcagcggcg ggagatgtgg
     2161 cgttttatga tgatgtgccg atggtcccgg tacttatcgt ctttattact ctggcgttgt
     2221 tataccgcct ggtaatgtgg ttgatggcgc acagtgaaaa actggaggat cttctggaag
     2281 gcaagccagt tgtcattatt gaagatggcg agctggcctg gtcgaaactc aataactcca
     2341 acatgacgga atttgagttc tttatggagc tacgattgcg tggcgtggag cagctggggc
     2401 aggtacgtct ggcgattctc gaaaccaacg ggcaaatcag tgtctatttc tttgaagatg
     2461 acaaggtgaa accgggttta cttattttac ccagtgattg tacgcagcgt tacaaagtgg
     2521 tgccggagtc ggcggactat gcctgcatcc gttgtagtga aatcattcat atgaacgcgg
     2581 gggaaaaaca attatgtccg cgctgtgcaa atccagaatg gacgaaggca agtcgggcta
     2641 aacgggtgac ctgacagcaa aaatatcggt tttttggtaa taatccgaga gattctgttg
     2701 tgtgacgcgt gccgcatttt tgccctcaac ggttttaccc attgcgatgc gtgtcactga
     2761 atgataaaac cgatagccac aggaataaca tattacccgc ggtcgcaatc gattgactgg
     2821 gggttaatgg caacgcaacg tggtgagggg aaatggctca aatcttcaat tttagttctg
     2881 gtccggcaat gctaccggca gaggtgctta aacaggctca acaggaactg cgcgactgga
     2941 acggtcttgg tacgtcggtg atggaagtga gtcaccgtgg caaagagttc attcaggttg
     3001 cagaggaagc cgagaaggat tttcgcgatc ttcttaatgt cccctccaac tacaaggtat
     3061 tattctgcca tggcggtggt cgcggtcagt ttgctgcggt accgctgaat attctcggtg
     3121 ataaaaccac cgcagattat gttgatgccg gttactgggc ggcaagtgcc attaaagaag
     3181 cgaaaaaata ctgtacgcct aatgtctttg acgccaaagt gactgttgat ggtctgcgcg
     3241 cggttaagcc catgcgtgaa tggcaactct ctgataatgc tgcttatatg cattactgcc
     3301 cgaatgaaac catcgacggt atcgccatcg acgaaacgcc agacttcggc aaagatgtgg
     3361 tggtcgccgc cgacttctct tcaaccattc tttcccgtcc gattgacgtc agccgttatg
     3421 gtgtaattta cgctggcgcg cagaaaaata tcggcccggc aggcctgaca atcgtcatcg
     3481 ttcgtgaaga tttgctgggc aaagcgaata tcgcgtgtcc gtcgattctg gattattcca
     3541 tcctcaacga taacggctcc atgtttaaca cgccgccgac atttgcctgg tatctgtctg
     3601 gtctggtctt taaatggctg aaagcgaacg gcggtgtagc tgaaatggat aaaatcaatc
     3661 agcaaaaagc agaactgctg tatggggtaa ttgataacag cgatttctac cgcaatgacg
     3721 tggcgaaagc taaccgttcg cggatgaacg taccattcca gttggcagac agtgcgcttg
     3781 acaaattgtt ccttgaagag tcttttgctg caggccttca tgcactgaaa ggtcaccgtg
     3841 tggtcggcgg aatgcgcgcg tccatttata acgccatgcc gctggaaggc gttaaagcgc
     3901 tgacagactt catggttgag ttcgaacgcc gtcacggcta atgccgaaat tttgcttaat
     3961 ccccacagcc agcctgtggg gtttttattt ctgttgtaga gagttgagtt catggaatcc
     4021 ctgacgttac aacccatcgc tcgtgtcgat ggcactatta atctgcccgg ttccaagagc
     4081 gtttctaacc gcgctttatt gctggcggca ttagcacacg gcaaaacagt attaaccaat
     4141 ctgctggata gcgatgacgt gcgccatatg ctgaatgcat tagcagcgtt aggggtaagc
     4201 tatacgcttt cagccgatcg tacgcgttgc gaaattatcg gtaacggcgg tccattacac
     4261 gcagaaggtg ccctggagtt gttcctcgga aacgccggaa cggcaatgcg cccgctggcg
     4321 gcagctcttt gtctgggtag caatgatatt gtgctgaccg gtgagccgcg tatgaaagag
     4381 cgcccgattg gtcatctggt ggatgcgctg cgcctgggcg gggcgaagat cacttacctg
     4441 gaacaagaaa attatccgcc attgcgttta cagggcggct ttacaggtgg caatgttgac
     4501 gttgatggtt ccgtttccag tcaattcctc accgcactat taatgactgc gccgcttgcg
     4561 ccggaagata cagttattcg tattaaaggc gatctggttt ctaaacctta tatcgacatc
     4621 acactcaatc tgatgaagac gtttggtgtt gaagttgaaa atcagcacta tcaacaattt
     4681 gtggtgaaag gtggtcaacc ttatcagtca ccgggtactt atttggtcga aggcgatgca
     4741 tcttcagctt cttacttcct ggcagcagca gcaatcaaag gcggaactgt aaaagtgacc
     4801 ggcattggac gcaacagtat gcagggtgat attcgctttg ctgatgtgct ggaaaaaatg
     4861 ggcgcgacca tttgctgggg cgatgattat atttcctgta cgcgtggtga actgaacgct
     4921 attgatatgg atatgaacca tattcctgat gcggcgatga ccattgccac ggcggcgtta
     4981 tttgcaaaag gcaccaccac gctgcgcaat atctataact ggcgtgttaa agaaaccgat
     5041 cgcctgtttg cgatggcaac agaactgcgt aaagtcggtg cggaagtaga agaggggcac
     5101 gattacattc gtatcactcc accggaaaaa ctgaactttg ccgagatcgc gacatacaat
     5161 gatcaccgga tggcgatgtg tttctcgctg gtggcgttgt cagatacacc agtgacgatt
     5221 cttgatccca aatgtacggc caaaacattt ccggattatt tcgagcagct ggcgcggatt
     5281 agccaggcag cctgaatgaa taacgtccaa taaatagcca aaactttctt tatcaaaacg
     5341 tcggcacatt gccggcgttt tttttcggac cttgtgagtc attttgatta atggtagcgt
     5401 cagttgtcaa tgtaagttgt tgatacataa tatttatata tgattaatca acggatgatt
     5461 cacatgaaga atactaaatt actgctggcg attgtgacct ctgcagcatt actgacgggg
     5521 tgtcaaaata cccacggtat tgataccaat atggctatca gctccggttt aaatgcctat
     5581 aaagcagcaa cattaagcga tgccgatgca aaagcgattg ccaatcaggg ctgtgccgaa
     5641 atggacagcg gcaatcaagt cgcaagtaaa tccagcaagt acggtaaacg tctggcaaaa
     5701 atcgccaaag cattgggtaa caatattaat ggcacgccgg tcaactataa ggtttatatg
     5761 accagcgacg tcaacgcatg ggcgatggcg aacggctgtg ttcgtgttta cagtggcctg
     5821 atggacatga tgaacgataa cgaaattgaa ggcgttctgg gccatgaact gggccacgtc
     5881 gcgttgggtc actcgctggc tgaaatgaaa gcttcttatg cgatcgttgc cgcacgcgat
     5941 gccatttcag ctaccagcgg tgtggcttcc cagctttccc gctcacaatt gggtgatatc
     6001 gcagaaggcg ctatcaatgc gaaatactcc cgcgataaag agtccgaagc agatgatttc
     6061 tcctttgatc tgttgaagaa acgtggcatc agcacccagg ggctggttgg cagcttcgaa
     6121 aaactggcta gcctggatgg cggtcgcacc cagtccatgt ttgactctca cccaccatca
     6181 acagagcgtg cacaacacat ccgtgatcgt atcgcctctg gtaagtaaat cattgtcatc
     6241 tttcgggctg gtcttctgcc agcccgctat aattgcgcaa taaatcccca tctgaataca
     6301 tacaaaactg gtttttgcac acaacgttaa cgatttgtgg cgtcggcgcg tataatgcgc
     6361 gcggttatgt taacggtacg cctgttttaa ggagataaag atgacggcaa ttgccccggt
     6421 tattaccatt gatggcccaa gcggtgcagg gaaaggcacc ttgtgtaagg ctatggcgga
     6481 agcgttgcaa tggcatctgc tggactcggg tgcaatttat cgcgtactgg cattggcggc
     6541 attacatcac catgttgatg ttgcgtcgga agatgcgctg gtaccgctgg catcccatct
     6601 ggatgtacgt tttgtgtcaa ccaatggcaa tctggaagtg atcctcgaag gggaagatgt
     6661 cagcggcgaa attcgtactc aggaagtggc gaatgcagct tcacaagtcg cggcattccc
     6721 acgcgttcgt gaagcattat tgcgtcgcca acgcgcgttt cgcgaattac caggcctgat
     6781 tgccgatggc cgcgacatgg gaacggtggt attccctgat gcgccagtga aaattttcct
     6841 tgacgcctcc tcggaagaac gtgcgcatcg ccgcatgcta cagttgcagg agaagggctt
     6901 tagtgttaac tttgagcgcc ttttggccga gatcaaagaa cgcgacgacc gcgatcgtaa
     6961 ccgagcggta gcgccactgg ttccggcagc cgatgcttta gtgttggatt ccaccacctt
     7021 aagcattgag caagtgattg aaaaagcgct acaatacgcg cgccagaaat tggctctcgc
     7081 ataagcgacc gaatttgcag tacccccgtt gcaatggaat gacagcgggt atgttaaaca
     7141 accccatccg gcatggagcc aggtggacgt taaatataaa cctgaagatt aaacatgact
     7201 gaatcttttg ctcaactctt tgaagagtcc ttaaaagaaa tcgaaacccg cccgggttct
     7261 atcgttcgtg gcgttgttgt tgctatcgac aaagacgtag tactggttga cgctggtctg
     7321 aaatctgagt ccgccatccc ggctgagcag ttcaaaaacg cccagggcga gctggaaatc
     7381 caggtaggtg acgaagttga cgttgctctg gacgcagtag aagacggctt cggtgaaact
     7441 ctgctgtccc gtgagaaagc taaacgtcac gaagcctgga tcacgctgga aaaagcttac
     7501 gaagatgctg aaactgttac cggtgttatc aacggcaaag ttaagggcgg cttcactgtt
     7561 gagctgaacg gtattcgtgc gttcctgcca ggttctctgg tagacgttcg tccggtgcgt
     7621 gacactctgc acctggaagg caaagagctt gaatttaaag taatcaagct ggatcagaag
     7681 cgcaacaacg ttgttgtttc tcgtcgtgcc gttatcgaat ccgaaaacag cgcagagcgc
     7741 gatcagctgc tggaaaacct gcaggaaggc atggaagtta aaggtatcgt taagaacctc
     7801 actgactacg gtgcattcgt tgatctgggc ggcgttgacg gcctgctgca catcactgac
     7861 atggcctgga aacgcgttaa gcatccgagc gaaatcgtca acgtgggcga cgaaatcact
     7921 gttaaagtgc tgaagttcga ccgcgaacgt acccgtgtat ccctgggcct gaaacagctg
     7981 ggcgaagatc cgtgggtagc tatcgctaaa cgttatccgg aaggtaccaa actgactggt
     8041 cgcgtgacca acctgaccga ctacggctgc ttcgttgaaa tcgaagaagg cgttgaaggc
     8101 ctggtacacg tttccgaaat ggattggacc aacaaaaaca tccacccgtc caaagttgtt
     8161 aacgttggcg atgtagtgga agttatggtt ctggatatcg acgaagaacg tcgtcgtatc
     8221 tccctgggtc tgaaacagtg caaagctaac ccgtggcagc agttcgcgga aacccacaac
     8281 aagggcgacc gtgttgaagg taaaatcaag tctatcactg acttcggtat cttcatcggc
     8341 ctggacggcg gcatcgacgg cctggttcac ctgtctgaca tctcctggaa cgttgcaggc
     8401 gaagaagcag ttcgtgaata caaaaaaggc gacgaaatcg ctgcagttgt tctgcaggtt
     8461 gacgcagaac gtgaacgtat ctccctgggc gttaaacagc tcgcagaaga tccgttcaac
     8521 aactgggttg ctctgaacaa gaaaggcgct atcgtaaccg gtaaagtaac tgcagttgac
     8581 gctaaaggcg caaccgtaga actggctgac ggcgttgaag gttacctgcg tgcttctgaa
     8641 gcatcccgtg accgcgttga agacgctacc ctggttctga gcgttggcga cgaagttgaa
     8701 gctaaattca ccggcgttga tcgtaaaaat cgcgcaatca gcctgtctgt tcgtgcgaaa
     8761 gacgaagctg acgagaaaga tgcaatcgca actgttaaca aacaggaaga tgcaaacttc
     8821 tccaacaacg caatggctga agctttcaaa gcagctaaag gcgagtaatt ctctgactct
     8881 tcgggatttt tattccgaag tttgttgagt ttacttgaca gattgcaggt ttcgtcctgt
     8941 aatcaagcac taagggcggc tacggccgcc cttaatcaat gcagcaacag cagccgctta
     9001 atttgccttt aaggaaccgg aggaatcatg accaagtcag aattgataga aagacttgcc
     9061 acccagcaat cgcacattcc cgccaagacg gttgaagatg cagtaaaaga gatgctggag
     9121 catatggcct cgactcttgc gcagggcgag cgtattgaaa tccgcggttt cggcagtttc
     9181 tctttgcact accgcgcacc acgtaccgga cgtaatccga agactggcga taaagtagaa
     9241 ctggaaggaa aatacgttcc tcactttaaa cctggtaaag aactgcgcga tcgcgccaat
     9301 atttacggtt aagtttttac tcaaacttga acgcgagaaa agcacctgtc gggtgctttt
     9361 ttcatttctc taatctggaa ctggaagctg cctcgcagag ttttgagcag ttttcaccct
     9421 ctcgttaaat tcttctgaat gtgcctcggg gaacgcaaaa ttcccaccag acaaccgctc
     9481 aacaaagttg cacactttcc ataaacaggg aggggtgcat gaaaataacg acagtcggcg
     9541 tatgcataat ttgcggaatt tttccgttgc tgattttgcc ccaattgcca gggacattaa
     9601 cccttgcgtt tctgactctc ttcgcctgcg tactggcatt tatccctgtt aaaacgctac
     9661 gttatatcgc gctgacgttg ctgtttttcg tttggggcat attatcagca aagcaaattt
     9721 tgtgggcagg agaaacctta actggcgcga cgcaggatgc aattgttgag atcactgcta
     9781 ctgacggcat gaccactcat tacggtcaaa ttactcatct acaaggtcga cgtatattcc
     9841 ctgcgccagg cctcgtgctg tatggcgaat atcttccgca agcggtttgt gctggacaac
     9901 aatggtcaat gaaactcaaa gttcgtgcgg ttcatggcca acttaatgat ggcggctttg
     9961 atagtcagcg ttatgccatt gccctgcatc agccgctcac cggccgcttt ctgcaggcaa
    10021 gtgttattga accgagttgt agcctgcgtg cacagtatct ggcgtcacta caaacaacgc
    10081 tgcaacccta tccgtggaat gcggttattc ttggtttagg tatgggggaa cggttatccg
    10141 tccctaaaga aatcaaaaat atcatgcgtg atactggaac ggcgcattta atggcgatat
    10201 cgggattgca catcgctttt gcggcgttgc tggctgccgg actcattcgc agtggacaaa
    10261 tttttctgcc tgggcgctgg atccactggc aaatgccatt aattggcgga atctgctgtg
    10321 ctacttttta tgcctggctg acgggaatgc aacctcctgc attgcgtacc gtcgtggcgc
    10381 ttgctacgtg gggaatgctt aagttaagtg ggcgacagtg gagtggctgg gatgtatgga
    10441 tatgttgtct ggcggcaatt ttgctgatgg atcctgttgc cattctctcg caaagtttat
    10501 ggctctctgc cgctgcggtc gcggcattga tattttggta tcagtggttt ccctgtcctg
    10561 agtggcaact gccgccggta ttgcgtgcag ttgtttccct catccatctg caactgggaa
    10621 tcacacttct gcttatgccc gtgcaaatcg tcatatttca tggcattagt ctgacctcgt
    10681 ttattgcaaa tctattagca attcccttgg tgacatttat cacggttccg ttgatcctcg
    10741 ccgcgatggt tgtgcattta agcgggccgt taatcctgga gcaagggtta tggtttcttg
    10801 ccgaccggtc tttggcttta cttttctggg ggttaaagag tttgccagaa gggtggatca
    10861 acattgctga acgttggcaa tggctatcat tttccccatg gttcttactg gttgtatggc
    10921 gattaaacgc ctggcgaacg ttgccagcaa tgtgtgtggc tgtaggcttg ctgatgtgct
    10981 ggccgctgtg gcaaaaacct cgacctgacg agtggcaagt gtacatgctt gatgtcgggc
    11041 aagggctggc aatggtgata gccagaaacg gcaaagcgat tctctatgac acgggactgg
    11101 cctggcctga aggggatagt gggcaacaac tgattatccc ctggctccac tggcataatc
    11161 ttgaaccgga aggcgttatc ctgagtcatg aacatctgga tcaccgggga gggctggact
    11221 caatattgca tacatggccg atgttatgga tcagaagtcc gttaaactgg gaacatcatc
    11281 agccctgtgt gcgtggcgaa gcgtggcaat ggcaaggatt gcgtttcagc gtgcactggc
    11341 ctttacaagc tagcaacgat aaaggaaata accattcctg tgtggttaag gttgatgacg
    11401 ggacgaatgg cattcttcta accggtgata ttgaagcccc agctgaacaa aagatgctaa
    11461 gccgttactg gcagcaagtg caggcaacat tgcttcaggt acctcaccat ggcagtaata
    11521 cctcatcatc gttgccatta attcagcgag tgaatggaaa agtggcactc gcatcggcat
    11581 cgcgctataa cgcatggcga ctgccctcta acaaagttaa gcatcgctat caacaacagg
    11641 gttatacgtg gcttgatact cctcatcagg ggcaagtaac ggtcgatttt tcagcgcaag
    11701 gctggcggat tagcagcctc agggagcaaa ttttacctcg ttggtatcat cagtggtttg
    11761 gcgtgccagt ggataacggg tagaatatgc ggctatttca acaaatgctg gttttttgaa
    11821 tgcataacga caaagatctc tctacgtggc agacattccg ccgactgtgg ccaaccattg
    11881 cgccttttaa agcgggtctg atcgtggcgg gcgtagcgtt aatcctcaac gcagccagcg
    11941 ataccttcat gctatcgctc cttaagccac ttcttgatga tggctttggt aaaacagatc
    12001 gctccgtgct ggtgtggatg ccgctggtgg tgatcgggct gatgattttg cgtggtatca
    12061 ccagctatgt ctccagctac tgtatctcct gggtatcagg aaaggtggta atgaccatgc
    12121 gtcgccgcct gtttggtcac atgatgggaa tgccggtttc attctttgac aaacagtcaa
    12181 cgggtacgct gttgtcacgt atcacctacg attccgaaca ggttgcttct tcctcttccg
    12241 gcgcactgat tactgttgtg cgtgaaggtg cgtcgatcat cggcctgttc atcatgatgt
    12301 tctattacag ttggcaattg tcgatcattt tgattgtgtt ggcaccgatt gtttcgattg
    12361 cgattcgcgt agtatcgaag cgttttcgca acatcagtaa aaacatgcag aacaccatgg
    12421 ggcaggtgac caccagcgct gaacaaatgc tgaaaggcca taaagaagta ttgattttcg
    12481 gtggtcagga agtggaaacg aaacgcttcg ataaagtcag caaccgaatg cgtcttcagg
    12541 ggatgaaaat ggtttcagcc tcttccatct ctgatccgat cattcagctg atcgcctctt
    12601 tggcgctggc gtttgttctg tatgcggcga gcttcccaag tgtcatggat agcctgactg
    12661 ccggtacgat taccgttgtt ttctcttcaa tgattgcact gatgcgtccg ctgaaatcgc
    12721 tgaccaacgt taacgcccag ttccagcgcg gtatggcggc ttgtcagacg ctgtttacca
    12781 ttctggacag tgagcaggag aaagacgaag gtaagcgcgt gctcgagcgt gcgactggcg
    12841 acgtggaatt ccgcaatgtc acctttactt atccgggacg tgacgtaccc gcattgcgta
    12901 acatcaacct gaaaattccg gcagggaaga cggttgctct ggttggacgc tctggttcag
    12961 gtaaatcaac catcgccagc ctgatcacgc gtttttacga tattgatgaa ggcgaaatcc
    13021 tgatggatgg tcacgatctg cgcgagtata ccctggcgtc gttacgtaac caggttgctc
    13081 tggtgtcgca gaatgtccat ctgtttaacg atacggttgc taacaacatt gcttacgcac
    13141 ggactgaaca gtacagccgt gagcaaattg aagaagcggc gcgtatggcc tacgccatgg
    13201 acttcatcaa taagatggat aacggtctcg atacagtgat tggtgaaaac ggcgtgctgc
    13261 tctctggcgg tcagcgtcag cgtattgcta tcgctcgagc cttgttgcgt gatagcccga
    13321 ttctgattct ggacgaagct acctcggcgt tggataccga atccgaacgt gcgattcagg
    13381 cggcactgga tgagttgcag aaaaaccgta cctctctggt gattgcccac cgcttgtcta
    13441 ccattgaaaa ggcagacgaa atcgtggtcg tcgaggatgg tgtcattgtg gaacgcggta
    13501 cgcataacga tttgcttgag caccgcggcg tttacgcgca acttcacaaa atgcagtttg
    13561 gccaatgatc gaaaaaatct ggtctggtga atcccctttg tggcggctat tgctgccact
    13621 ctcctggttg tatggcctgg tgagtggcgc gatccgtctt tgctataaac taaaactgaa
    13681 gcgtgcctgg cgtgcccccg taccggttgt cgtggttggt aatctcaccg caggcggcaa
    13741 cggaaaaacc ccggtcgttg tctggctggt ggaaaagttg caacagcgcg gtattcgcgt
    13801 gggggtcgta tcgcggggat atggtggtaa ggctgaatct tatccgctgt tattgtcggc
    13861 agataccacc acagcacagg cgggtgatga acctgtgttg atttatcaac gcactgatgc
    13921 gcctgttgcg gtttctcccg ttcgttctga tgcggtaaaa gccattctgg cgcaacaccc
    13981 tgatgtgcag atcatcgtaa ccgacgacgg tttacagcat taccgtctgg cgcgtgatgt
    14041 ggaaattgtc gttattgatg gtgtgcgtcg ctttggcaat ggctggtggt tgccggcggg
    14101 gccaatgcgt gagcgagcgg ggcgcttaaa gtcagttgat gcggtaatcg tcaacggcgg
    14161 tgtcccccgc agcggtgaaa tccccatgca tctgctgccg ggtcaggcgg tgaatttacg
    14221 taccggtacg cgttgtgacg ttgctcagct tgaacatgtg gtggcgatgg cagggattgg
    14281 gcatccgccg cgcttttttg ccacgctgaa gatgtgcggt gtacaaccgg aaaaatgtgt
    14341 accgctggcc gatcatcagt ctttgaacca tgcggatgtc agcgcgttgg taagcaccgg
    14401 gcaaacgctg gtaatgactg aaaaagatgc ggtgaaatgc cgggcctttg cagaagaaaa
    14461 ttggtggtat ttgcccgttg acgcacagct ttcaggtgat gaaccagcga aactgcttgc
    14521 gcaactaacc tcgctggctt ctggcaacta gttacgccgc ggcagcgttc gattgatgga
    14581 gtcatgaatg tcgctgccgc acctctccct tgctgatgcg cgtaatcttc accttgccgc
    14641 gcaaggcctg ttaaacaaac cccgccgtcg agcgtcgttg gaggatattc cggcaacgat
    14701 ctcccgcatg tccttgctgc aaatcgatac catcaatatt gttgcccgta gcccatatct
    14761 ggtgcttttc agtcgtctgg gaaattatcc tgcccagtgg ctggatgagt ctctggcgcg
    14821 tggcgaatta atggaatact gggcgcatga agcctgcttt atgccacgca gcgactttcg
    14881 tcttattcgc caccgcatgc tggcacctga aaaaatgggc tggaaataca aagacgcctg
    14941 gatgcaggaa catgaggcgg aaattgcaca gttaattcag catattcatg ataaggggcc
    15001 ggtacgttca gccgattttg agcatcctcg taaaggtgca agcggctggt gggaatggaa
    15061 gccgcataaa cggcatctgg aaggtttatt tactgccgga aaggtgatgg tgattgaacg
    15121 gcgcaacttc cagcgcgttt atgatttaac ccaccgtgtc atgcctgact gggatgatga
    15181 gcgcgatctc gtttcgcaaa cagaagcaga aatcatcatg ctggataaca gtgcgcgtag
    15241 cctgggaata ttccgcgaac agtggctggc agattactat cggctgaaac gtccggcact
    15301 ggcggcgtgg cgtgaagcga gggctgaaca gcagcaaatc attgctgtgc atgttgaaaa
    15361 attgggcaat ctttggctgc atgctgattt gctgccgcta ctcgagcgag cgctggccgg
    15421 aaagctcact gcaacgcaca gcgcggtact ttcgcctttt gatcctgttg tctgggatcg
    15481 caaacgcgca gagcagcttt ttgattttag ctaccggctg gagtgctata ccccagcgcc
    15541 gaaacgccag tatggctatt ttgttctgcc gttattacat cgtgggcaat tagttgggcg
    15601 aatggatgcc aaaatgcatc gccagacagg catccttgaa gttatctctc tgtggttaca
    15661 ggaaggtatt aaaccaacga caacgctgca aaaagggtta cgtcaggcga ttactgattt
    15721 cgctaactgg cagcaggcaa cgcgggtgac attaggacac tgcccgcaag gtctctttac
    15781 ggattgccgc accggctggg aaatagaccc cgtcgcataa atgattatga taagatttat
    15841 gcattcataa cccgatccat ctggaggaac tatggatcat cgtctgcttg aaatcattgc
    15901 ctgcccggtt tgcaacggaa aactttggta taaccaggaa aaacaagaac ttatttgcaa
    15961 actggacaac ctagctttcc cgctgcgcga tggcattcca gtgttgctgg aaacggaagc
    16021 ccgcgtgctg actgctgatg agagtaaatc atgagttttg tggtcattat tcccgcgcgc
    16081 tacgcgtcca cgcgtctgcc cggtaaacca ttggttgata ttaacggcaa acccatgatt
    16141 gttcatgttc ttgaacgcgc gcgtgaatca ggtgccgagc gcatcatcgt ggcaaccgat
    16201 catgaggatg tcgcccgcgc cgttgaagcc gctggcggtg aagtatgtat gacgcgcgcc
    16261 gatcaccagt cagggacaga acgcttggcg gaagttgtcg aaaaatgcgc gttcagcgac
    16321 gacacggtga tcgttaatgt gcagggtgat gaaccgatga tccctgcgac aatcattcgt
    16381 caggttgctg ataacctcgc tcagcgtcag gtgggtatgg cgactctggc ggtgccaatc
    16441 cacaatgcgg aagaagcgtt taacccgaat gcggtgaaag tggttctcga cgctgaaggg
    16501 tatgcactgt acttctcacg cgctaccatt ccgtgggatc gtgatcgttt tgcaaaaggc
    16561 cttgaaaccg ttggcgataa cttcctgcgt catcttggta tttatggcta ccgtgcaggc
    16621 tttatccgtc gttacgtcac ctggcagcca agcccgttag aacacatcga aatgttagag
    16681 cagcttcgtg ttctgtggta cggcgaaaaa atccatgttg ctgtcgcgca tgaagttcct
    16741 ggcacaggtg tggatacccc tgaagatctc gagcgcgttc gcgctgaaat gcgctaattt
    16801 cacttcacga cactccagcc aattttggga ggagtgtcgt accgttacga ttttcctcaa
    16861 tttttctttt caacaattga tctcattcag gtgacatctt ttatattggc gctcattatg
    16921 aaagcagtag cttttatgag ggtaatctga atggaacagc tgcgtgccga attaagccat
    16981 ttactgggcg aaaaactcag tcgtattgag tgcgtcaatg aaaaagcgga tacggcgttg
    17041 tgggctttgt atgacagcca gggaaaccca atgccgttaa tggcaagaag ctttagtacg
    17101 cccggaaaag cccgacaact ggcatggaaa accaccatgc tggcaagaag tgggactgtc
    17161 cgtatgccga ctatttatgg tgtgatgacg catgaagaac accccggccc cgatgtcctg
    17221 ctactggagc ggatgcgtgg tgtttcggtg gaggcaccag cccgaacacc agaacgctgg
    17281 gaacagctca aagaccaaat cgttgaagcc ttactggcct ggcaccgtca ggacagtcgc
    17341 ggttgcgtcg gcgcggtcga caatactcag gaaaatttct ggccctcatg gtaccggcaa
    17401 catgttgaag tgctatggac cacgctcaat cagttcaata acaccggtct gacgatgcag
    17461 gataagcgga tcctgtttcg cactcgcgaa tgtctcccgg cattatttga aggttttaac
    17521 gacaattgtg tgctgattca cggtaacttc tgtttacgca gcatgctgaa agattcgcgc
    17581 agcgatcagt tactggcgat ggtcgggccg ggactaatgc tttgggcacc gcgagaatac
    17641 gaactgttcc gactaatgga taattctctg gcggaagatt tgctctggag ttacctgcaa
    17701 cgcgcgccag tggcggagtc gttcatctgg cggcgttggt tgtatgtgtt atgggatgaa
    17761 gttgcgcaac tggttaatac cggacgattt agtcggcgca acttcgatct ggcatcaaaa
    17821 tcactcttgc cgtggctcgc ctgacggacc tttcagccac tgccagatgc ggccaagcgt
    17881 ttcatagcca acacgatcgc tatgcatcag ccagaccgga gaagggattg cccgttccca
    17941 cggattaagc ggcgagtcga tggccaactg attcgccggg gctggtagcg gatttaaccc
    18001 ttcctgctga aaaaagatca tcgcccgcgg cagatgtgat gcggaggtca ccagcaggaa
    18061 aggggcgtca ccaatcgcct gtttcactgc cgcagcttct tcttcggtat cttttggcaa
    18121 atccagggtg ataatttgct cgcgcggtac gcccagcgat tgcgcaactc tggcacctac
    18181 ttccgctgta ctcaccgtat tggtttttgc tacgcctccc gtgaagatca gttttgaacc
    18241 cggattttcg cgccataaac ggataccctc attcaggcgt ggcagactgt tattgattaa
    18301 attagagctc ggtgcccact gcgggttcca ggtataacca cctccgagca ccacgatata
    18361 gtccactttt tgcgaattat tccatgtcgg ataggtgctt tcgatgggac gcagcaggcg
    18421 gtcggctacc ggttgcaaac tcaataacaa gagtgccagc cacccaatgc taataaaaat
    18481 cttcccggtt ttttgaaaac ggctaaacca cagcagcgcc aggccagcgc caataattaa
    18541 caacatcagc ggaagtggca gcaacatatt gccaatcact tttttcagtg taaaaagcat
    18601 cctttctggt tcctttttta accatatagc aagggatcgc cagcgatatt acaccagacg
    18661 ggttcattct cgccgtggct gtgacaaaat agcgtcttta tcagaaaacg tcttagccaa
    18721 ccggtggaga aagcaatgca ggatcgcaat tttgatgata ttgcggaaaa gttttcccgt
    18781 aacatttacg gcaccaccaa agggcagctt cgacaggcta ttctgtggca ggatctcgat
    18841 cgcgtgctgg cggaaatggg gccgcaaaaa ctgcgtgtgc tggatgcggg cggtggagaa
    18901 gggcagaccg caatcaaaat ggccgagcgc gggcatcagg tcattttatg cgatctttct
    18961 gcgcagatga tcgaccgcgc aaaacaggcg gcagaagcaa aaggtgtgag cgacaacatg
    19021 caatttatac attgcgccgc tcaggatgtt gcttcgcatt tggaaacgcc cgttgatctg
    19081 atattgttcc atgcggtgct cgagtgggtg gctgatcccc gcagcgtatt gcagaccctg
    19141 tggtcagtgt tgcatccagg cggcgtgttg tcgttaatgt tctacaatgc gcatggtttg
    19201 ttgatgcata acatggtcgc cgggaatttt gattacgtgc aggcgggaat gccgaaaaag
    19261 aaaaaacgga cgctttcgcc agattatcca cgcgacccgg cgcaggttta tctgtggttg
    19321 gaagaagctg gttggcaaat tatgggtaag acaggcgttc gcgtgtttca tgattatctg
    19381 cgcgagaagc accagcagcg cgactgctat gaagcattac ttgaattaga aacgcgttat
    19441 tgccgtcagg aaccgtatat taccctgggg cgttatattc atgtcaccgc gcgcaaaccg
    19501 cagagcaagg ataaagtatg agtgaatttt cccagacagt ccccgaactg gttgcctggg
    19561 ccagaaaaaa tgacttctcc atttcgctgc cggtagaccg actctctttt ctgctggcgg
    19621 ttgccacgct gaacggcgag cgtctggatg gtgagatgag tgaaggcgag ctggtggatg
    19681 cattccgcca tgtgagtgat gcgtttgagc aaaccagcga aaccatcggc gtgcgcgcca
    19741 ataacgcgat caacgacatg gtgcgtcaac gtctgctgaa ccgctttacc agcgagcagg
    19801 cagaagggaa cgcaatttac cgtctgacgc cgctcggcat cggcattact gactattaca
    19861 tccgtcagcg cgagttttct acgctgcgcc tctctatgca gttgtcgatt gtggcgggtg
    19921 agctcaaacg cgcagcagat gccgccgaag agggcggtga tgaatttcac tggcaccgta
    19981 atgtttatgc gccactgaaa tattcggtag cagaaatttt cgacagtatc gacctgacgc
    20041 aacgtctgat ggacgaacag cagcagcagg tgaaggacga tatcgcccag ttgctgaaca
    20101 aagactggcg ggcggcgatt tccagctgtg aattgttgct ttcggaaact tccggaacgc
    20161 tgcgtgaatt gcaggatacg ctggaagcgg caggcgacaa attgcaggct aatctgttgc
    20221 gcattcagga tgcgacgatg acccatgacg atctgcattt tgtcgatcgt ctggtgttcg
    20281 atctgcagag caaactcgac cgtattatca gttggggcca gcaatccata gacttgtgga
    20341 ttggctacga ccgccacgta cacaaattta tccgtaccgc aatcgatatg gataaaaacc
    20401 gcgtctttgc tcagcggtta cgtcagtcgg tacaaaccta ttttgatgag ccgtgggcgc
    20461 taacttatgc caatgccgat cgtctgctgg atatgcgtga cgaagagatg gcactgcgcg
    20521 atgaagaagt gactggagaa cttcctgagg atctggagta cgaagagttt aacgagatcc
    20581 gcgaacaact ggcggcgatc atcgaagaac aactcgccgt gtacaaaacc agacaagtgc
    20641 cgctggatct tggtctggtg gtacgcgaat atctgtcaca gtatccgcgt gcacgtcact
    20701 ttgacgttgc gcgcattgtt attgatcagg cggtacgtct tggcgtagcg caagcagatt
    20761 tcaccggact gccagcgaaa tggcagccga ttaatgatta cggagccaag gtacaggcgc
    20821 atgtcatcga caaatattga acaagtgatg ccggttaagc tggcgcaggc gctggcgaat
    20881 ccgttatttc cggcgctgga cagcgcctta cgttcaggcc gccatattgg cctcgacgaa
    20941 ctggataatc atgcattctt gatggatttt caggaatatc tggaagagtt ttacgcgcgt
    21001 tataacgttg agcttattcg cgcaccggaa gggttcttct atttacgccc acgttccacc
    21061 actcttatcc cgcgttccgt cttgtcggaa ctggatatga tggtcgggaa aatcctctgt
    21121 tatctctatc tcagcccgga acggctggcg aatgagggga ttttcaccca gcaggaactg
    21181 tacgacgaac tgctcaccct ggccgatgaa gcgaaactgc tgaaactggt gaacaaccgt
    21241 tcaaccggtt cagacgttga ccgtcagaag ttgcaggaga aagtacgttc ttcgctcaac
    21301 cgtctgcgtc gtttaggcat ggtgtggttt atgggccacg acagcagcaa gttccgcatt
    21361 accgaatcgg tgttccgctt cggagccgat gtgcgtgctg gcgacgatcc ccgtgaagca
    21421 cagcgtcgcc tgattcgtga tggcgaagca atgccgattg aaaatcatct gcaactcaac
    21481 gatgaaaccg aagagagtca gccagatagc ggagaggaag aataatgatt gaacgcggta
    21541 aatttcgctc actgacgctg attaactgga acggcttttt tgcccgaact tttgaccttg
    21601 acgagctggt cacgacgctt tccggcggta acggggcggg taaatccacc acgatggcgg
    21661 cgttcgttac ggcgctgatc cccgacctga ccctgctgca tttccgtaac actacggaag
    21721 ccggggccac cagcggttcg cgcgataaag gtctgcacgg taagctgaaa gcgggtgtct
    21781 gttattcgat gctcgacacc attaactcgc gccaccagcg cgtggtggtc ggtgtgcgtc
    21841 tgcaacaggt tgccggacgc gatcgtaaag tggatatcaa accatttgcc attcagggac
    21901 tgccgatgtc ggtgcagccg acacagctgg tgaccgaaac tctgaacgaa cgccaggcgc
    21961 gcgtgctgcc gcttaacgag ctgaaagaca agctcgaggc gatggaaggc gtgcagttta
    22021 aacagttcaa ctccattacc gattaccact cgctgatgtt cgatctgggc atcattgcgc
    22081 gtcgtctgcg ctccgcatct gaccgtagca aattctatcg tctgatcgaa gcttcgctgt
    22141 atggcgggat ctccagtgcc attacccgtt ctctgcgcga ctacctgttg ccggaaaaca
    22201 gcggcgtgcg taaagcgttc caggacatgg aagcggcgct gcgtgaaaac cgcatgacgc
    22261 tggaagcgat tcgtgtcacc cagtcggacc gtgacctgtt taagcatctg atcagcgaag
    22321 ctaccaacta cgtggcggcg gactacatgc gtcacgccaa cgagcgccgt gtccatctcg
    22381 ataaggccct ggagtttcgt cgcgaactac atacttcgcg tcagcaactg gcggcggagc
    22441 agtacaaaca cgtcgatatg gcgcgtgagc tggcagagca caacggtgcc gaaggcgatc
    22501 tggaagcgga ttatcaggcg gccagcgatc acctgaacct ggtgcaaacc gcactgcgtc
    22561 agcaggagaa gatcgaacgc tacgaagcgg atctcgatga gctgcagatc cgtctggaag
    22621 agcaaaacga agtggtggca gaagccatcg aacgccagga agagaacgag gctcgcgcgg
    22681 aagctgccga actggaagtg gacgagctga aaagccagct tgctgactac cagcaggcgc
    22741 tggacgtcca gcaaacgcgc gcgatccagt acaaccaggc gattgctgcg cttaatcgtg
    22801 ccaaagaact gtgccatctg ccggacttaa ccgccgacag cgccgccgaa tggctggaaa
    22861 ccttccaggc gaaagagctg gaagcgactg aaaaaatgct ctctcttgag cagaaaatga
    22921 gcatggcgca aaccgcgcac agccagtttg agcaggctta tcagctggtg gtggcaatca
    22981 acggcccgct ggcgcgtaac gaggcgtggg atgtcgctcg cgaactattg cgcgaagggg
    23041 tcgatcagcg tcacctggca gagcaggttc agccgttgcg gatgcgctta agcgaactgg
    23101 aacagcgtct gcgcgaacag caagaagctg agcgtctgct ggcagatttc tgcaaacgtc
    23161 agggcaagaa ttttgacatt gacgagctgg aagccctgca tcaggagctg gaagcacgca
    23221 ttgcctctct ttccgatagc gtgtctaacg cccgtgaaga gcggatggca ctgcgccagg
    23281 agcaggaaca gctgcagtct cgcattcaga gtttgatgca gcgtgcaccg gtttggctgg
    23341 cagcgcaaaa cagtctcaac cagttgagcg aacagtgcgg cgaagagttc acctccagcc
    23401 aggacgtcac tgaatatctg caacagttgc tggagcgtga gcgcgaggcc attgttgaac
    23461 gcgatgaagt aggcgcgcgc aaaaacgccg tcgatgaaga gatcgaacgt ttaagccagc
    23521 ctggcggctc tgaagatcag cgtctgaacg cgctggcgga gcgttttggt ggtgtgctgc
    23581 tgtcagaaat ttatgacgac gttagcctgg aagatgcgcc gtacttctcg gcgctgtatg
    23641 gcccgtcacg ccacgccatc gtggtgccag atctgtcaca ggtaaccgaa cacctggaag
    23701 gtttgaccga ttgcccggaa gatctctatt tgatcgaagg ggatccgcag tcattcgatg
    23761 acagcgtgtt cagcgttgat gagctggaaa aagcggtggt ggtgaaaatc gccgatcgac
    23821 agtggcgtta ttcacgtttc ccggaagtgc cgctgtttgg tcgtgctgcg cgtgaaagcc
    23881 gtattgaaag cctccatgcc gagcgtgaag tgctttccga acgcttcgcc acgctctcct
    23941 ttgatgtaca gaaaacccag cgtctgcatc aggcgttcag ccgctttatc ggcagtcatc
    24001 tggcggtggc gtttgagtct gacccggaag cagaaatccg tcaactgaac agccgtcgag
    24061 tcgaactgga gcgggcgttg agtaatcatg aaaatgataa ccagcagcag cgtattcagt
    24121 ttgagcaggc gaaagaaggc gttacggcgc tgaaccgcat tctgccgcgt ctcaacctgt
    24181 tggctgatga cagcctggcg gatcgcgtcg atgaaatccg cgaacgtctg gatgaagccc
    24241 aggaagccgc gcgttttgtt cagcagtttg gcaatcaact ggcgaaactg gaaccgatcg
    24301 tttcggtatt gcagagcgac ccggaacagt tcgaacagtt aaaagaagat tacgcgtact
    24361 ctcagcagat gcagcgcgat gcccgtcagc aagcgtttgc cctgacggaa gtggtgcagc
    24421 gtcgtgcgca ctttagctat tctgactcgg cagaaatgct tagcggtaac agcgatctca
    24481 acgaaaaact gcgtgaacgt ctggaacagg cggaagcgga gcgtacccgc gctcgcgaag
    24541 cgttgcgcgg tcacgcagcg cagttgagtc agtacaacca ggtgctggct tcgctgaaaa
    24601 gttcttacga caccaaaaaa gagctactca acgatctgca acgtgaattg caggatatcg
    24661 gcgtgcgtgc tgatagcggg gcagaagagc gggcgcgtat tcgccgtgac gagctgcatg
    24721 cgcaactgag caataaccgt tcacgccgca atcaactgga aaaagcgctt accttctgcg
    24781 aagcggagat ggacaacctg acccgcaaac tgcgcaagct ggagcgggat tactttgaga
    24841 tgcgcgagca ggtagtgacc gccaaagcgg gctggtgtgc ggtgatgcgc atggtgaaag
    24901 ataacggcgt tgagcgccgc ttacaccgtc gtgagctggc ttatctctcc gctgatgatt
    24961 tgcgttccat gtcggataag gcgttaggtg cgctgcgtct ggcggtggcg gataacgaac
    25021 atctgcgcga cgtgctgcgc atgtcggaag atccgaaacg tccggagcgt aaaattcagt
    25081 tcttcgtggc ggtttatcag catctgcgtg aacgtattcg tcaggatatt attcgtaccg
    25141 atgatccggt ggaagctatc gaacagatgg agattgaact tagccgtctg accgaagaat
    25201 taacctcccg tgaacagaaa ctggcgatca gttcccgcag cgtggcgaac atcattcgca
    25261 aaaccattca gcgcgagcag aaccgtatcc gtatgctcaa ccaggggttg cagaacgtat
    25321 cgtttggtca ggtgaacagc gtgcgcctca acgtgaacgt gcgtgaaacg cacgccatgc
    25381 tgctggatgt gctctctgaa cagcacgagc agcatcagga tctgtttaac agcaaccgtc
    25441 tgaccttctc ggaagcgttg gcgaaattgt atcagcgtct taatccgcag attgatatgg
    25501 ggcagcgcac gccgcagacc attggcgaag aactgctgga ttaccgcaac tatctggaaa
    25561 tggaagttga ggttaaccgt ggttccgatg gttggttgcg cgcagagtct ggtgcattgt
    25621 cgaccggtga ggcgattggt accggtatgt cgattctggt gatggtggta caaagctggg
    25681 aagatgaatc tcgccgcctg cgcggtaaag atatctctcc ttgccgcctg ctgttcctcg
    25741 atgaagcagc gcgactggat gctcgttcta tcgccacgct gtttgaattg tgtgaacgtt
    25801 tgcaaatgca gctcatcatc gcagcgccgg aaaatatcag cccggagaaa ggcaccacct
    25861 ataaactggt gcgtaaagtc ttccagaata ccgaacacgt tcacgtcgtc ggcctgcgag
    25921 gatttgcgcc gcaactccct gaaacgcttc caggaagtga cgaagcgcct tctcaggcga
    25981 gttaaaatta aggcggcagc aatgccgcct tttctttttc cgaaaactcc gtttctgcac
    26041 taaaaaagtg gcacattacg gcgcgctttt ctttaaactt ctttacatta ggttatgtaa
    26101 aaacgtatcg gcgtttatat actgaagata agcctgatga gtaacaggct tgctcgtcat
    26161 actttcgtga gtattggcgt tgtacaggca agtcgtaaaa taacagcctg gctattcaga
    26221 gtatgataaa aacagggggc aagggatgtt gcttaatatg atgtgtggtc gtcggctgtc
    26281 ggcaatcagt ttgtgcctgg ccgtaacatt cgctccactg ttcaatgcgc aggccgatga
    26341 gcctgaaata atccctggcg acagcccggt ggctgtcagt gaacagggcg aggcactgcc
    26401 gcaggcgcaa gccacggcaa taatggcggg gatccagcca ttgcctgaag gtgcggcaga
    26461 aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt tacaagccgg tttatcttaa
    26521 ccagcttcaa ctgttgtatg ccgcacgcga tatgcaaccc atgtgggaaa accgtgatgc
    26581 tgttaaagcc ttccagcaac agctggctga ggtggcgatt gctggtttcc agccgcagtt
    26641 taataaatgg gtagagttac tgaccgatcc tggtgttaac gggatggcac gtgacgtggt
    26701 gctctctgat gcgatgatgg gctatctcca tttcattgca aatattccgg tcaaaggcac
    26761 tcgctggcta tatagcagta aaccttatgc gctttcaacg ccgccgcttt cggtgattaa
    26821 ccaatggcag ctagcgctgg ataaaggtca attgcctacg tttgttgcag gactggcacc
    26881 gcagcatccg caatatgcgg cgatgcatga atcgttacta gccttactca gtgacaccaa
    26941 accgtggccc caactgaccg gcaaagcaac gttgcgccca gggcagtgga gtaacgacgt
    27001 accggcgttg cgtgaaatat tgcaacgcac aggcatgttg gacggggggc cgaaaattac
    27061 tctacctggc gatgacacgc caactgacgc ggtagtcagc ccatccgctg ttactgttga
    27121 aacagcagaa actaagccga tggacaagca aacgacgtct cgtagtaaac ctgcgcctgc
    27181 cgttcgtgcc gcctacgata atgaactggt ggaagccgtt aaacgttttc aggcatggca
    27241 aggattgggg gcagatggtg ctattggccc ggcaacgcgg gactggttaa acgtaacgcc
    27301 cgcccagcgt gctggcgtgt tggcactcaa catccagcga ttgcgcttgc tgccaacaga
    27361 gctttctacc gggatcatgg ttaacattcc ggcctattcg ctggtctact atcagaacgg
    27421 caatcaggtg ctggattcgc gagtcattgt cggtcgcccc gatcgcaaaa cgccgatgat
    27481 gagcagtgcc cttaacaacg tagtggtaaa cccgccgtgg aacgtaccac caactctggc
    27541 acgcaaagat attctgccga aagtgcgcaa cgatccggga tatctcgaaa gccatggcta
    27601 tacggtgatg cgcggctgga acagcagaga agcgattgac ccatggcagg ttgactggtc
    27661 tacaatcacg gcctcgaatt taccgtttcg cttccagcag gctccaggcc cacggaactc
    27721 gctggggcgc tataaattca atatgccgag ttcagaggcc atttatttgc atgacacgcc
    27781 gaaccacaat ctgttcaagc gtgatacacg cgcattgagc tcaggctgtg tacgagtgaa
    27841 taaagcttcc gatctggcga atatgctgtt gcaggatgca ggctggaatg acaaacgtat
    27901 ttctgatgcg ctgaagcagg gtgatacacg ttacgtcaat attcggcagt cgattccggt
    27961 gaatctctac tacctgacgg cctttgttgg tgcagatggt cgtacccagt atcgtacaga
    28021 tatttacaat tatgatctgc ctgcgcgatc cagctcgcaa atcgtatcga aagcggaaca
    28081 attaatcagg taaatgaagt agttcgggga aatcagttgt cgtaataatt agcatgattg
    28141 ggggtggttc tctgcagccc ccgtcactgc tggggttgag tcatcttgac gtctgcttta
    28201 cgggcggtta aggtgcctct tgtgcgccag aagtgcatat aaacgataac attgacctgt
    28261 agacttgatt atcatggaca aattcgacgc taatcgccgc aaattgctgg cgcttggtgg
    28321 cgttgcactc ggtgccgcca tcctgccgac ccctgcgttt gcaacactct ctaccccacg
    28381 cccgcgcatt ttgacactca ataatcttca taccggagag tcaatcaaag cggagttttt
    28441 cgatggcaga ggctatattc aggaagaatt ggcaaaactt aaccattttt tccgcgatta
    28501 ccgcgcgaac aaaataaagt ccatcgaccc aggattattc gaccagttgt atcgcctgca
    28561 agggttgtta ggcacgcgca aaccggtgca actcatttcc ggttatcgtt ccattgatac
    28621 caacaatgaa ctacgcgccc gcagccgtgg agtagcgaag aaaagctatc acactaaagg
    28681 ccaggcgatg gatttccata ttgaaggtat cgcgttaagc aatattcgca aagccgcgtt
    28741 atctatgcgc gcaggtggtg taggatatta cccacgtagt aactttgtgc atattgatac
    28801 cgggccagca cggcactggt agtaattgct taacgaaaca ggggcagtat gaactatcgt
    28861 attattccgg tcaccgcatt ctcccagaac tgttcattga tctggtgtga acaaacccgc
    28921 ctggccgcac tggtcgatcc tggcggcgat gcggaaaaaa tcaaacagga agttgatgac
    28981 agcggcctga cactgatgca gatcctgctg acgcatggtc atctggacca cgttggcgca
    29041 gcggcggaac tggcgcaaca ttacggcgtg ccggtttttg gtccggaaaa agaagatgag
    29101 ttctggctgc aaggcttgcc tgcgcaaagt cgtatgtttg gtctggaaga gtgccagccg
    29161 ctgacgccag atcgttggct gaacgaaggc gataccatca gcatagggaa tgtgacttta
    29221 caggtgttac attgccctgg gcatacgccg ggtcatgtcg tgttttttga tgatcgggca
    29281 aagctgctga tttctggcga tgttattttc aaaggcggag tagggcgcag tgacttcccg
    29341 cgtggcgatc ataatcaact gatttcttca atcaaagata aattgctgcc actgggggat
    29401 gacgtgacat ttattccggg tcacggacca ttatccacac ttggttatga acgcctgcat
    29461 aatcccttcc tgcaagacga aatgcccgtc tggtaaggca cacataaaaa agcccgcttt
    29521 tcagcgggct tcattgtttt taacgcttac agcactgcca caatcgcttc gcacagcgga
    29581 gccatgttat ctggtgtcat cccggccacg tttacgcgac cagaagcaac agcatacacg
    29641 ccaaactctt cgcgcagacg cagcacttgt tcttttgtca gaccactgaa ggagaacatg
    29701 ccgttctgtt tgatgataaa gctgaagtca cggtttgcgc ctttttcctg cagcgtattg
    29761 acgaacaact gacgcatacg ctgaatacgc tggcgcatat cagtcagctc ttgttcccaa
    29821 atcgcacgta atgcatcgtt gctcaggatg gtggcaacaa cagaagcgcc gtgtgctgga
    29881 gggttagagt agttagcgcg aatcgccgct ttcatttggc tgaatgcgcg atcaacggtt
    29941 tcactgtcgg cagcaaccag agtacaagcg ccaacacgct cgttgtacag gccaaagttt
    30001 ttagagtagg aactggcaac aatcagctct ttatgcatag ccgcgaaagc gcgcagtcct
    30061 tcagcatctt cttccagacc acgggcaaaa ccctggtaag cgaagtcaaa cagcggtaac
    30121 cagcctttct caacggagag ttgtgccagt gtctgccatt gttccagcgt agggtcgata
    30181 ccggttgggt tatggcagca gccatggaac agcactacat cgccagcctg agcttcgttc
    30241 agactgttaa tcagtgcatc gaagtcaagg gtgtgatttt ccgcatcata ataagcgtat
    30301 tcacgaactt ccagacctgc agagttaaag acgctcttat ggttcggcca gcttgggttg
    30361 ctcacccaca cacgcttaac gctggtattt tttgccagga aatcggcagc cacgcgtagt
    30421 gcgccagtgc cacccggagt ctgtgccgtg cgagcacgtt tgtcattgat cagggcgcta
    30481 cctttaccaa acagcagttc ctgagtgcag cgaccaaatt cagggatgcc gtcaatgccg
    30541 aggtaatttt tggtggtttc attttcgagc agatactgtt cagccttttt cacgctggtc
    30601 agtaccgggg ttttgcccgt ctcatcttta tagacaccaa tcccgaggtt aattttgccg
    30661 ggacgttcat cggcacgaaa cagatcggcc aggcccagaa tcgggtcggc aggagcggcg
    30721 gtaatgttct caaacatgac gaggttccat tatggttaca gaagggaagt ccgctatcag
    30781 ggtaacggga gatttacaaa attccaacta ttactgatga aaacgcaggc tgtttttgca
    30841 agacgtgaga ttgctctgga aggcataaaa aaaacaggac caacgtcctg ttttttcggc
    30901 atttaacaaa gaggtgtgct attagaactg gtaaacgata cccacagcaa cggtgtcgtc
    30961 agaacctacg cccagtttgt tgtcagaatc gatctggttg atgatgtagt caacataggt
    31021 ggacatgttt ttgttgaagt agtaggttgc gcccacttca aagtagttca ccagatcaac
    31081 atcaccgata ccttctacgt ctttcgcttt agatttggtg taagcgatgg acggacgcag
    31141 accgaaatcg aactggtatt gcgcaactaa cagaacgtct tgcgttttgt tggcgaagcc
    31201 gccttcaatt ggcgtagcgt tacgggtttc accgtagttc gctgccaggt agatgttgtt
    31261 cgcgtcatac ttcagaccag ttgcccactg ttcagctttt ttaccctgac cgcggaattc
    31321 ggcttcctgg gcgtcggtac ggtctgctgc accgtaagca cctacgatac caaagccttc
    31381 gaattcgtag ctgatagagc caccgacgcc gtcgccgttg gaacgctcag gaataccagc
    31441 acgttcgttt ttgcccaggt actgaacggc aaagttcagg ccgtcaacca gaccgaagaa
    31501 gttggagtta cgataggtag caacgccgcc tacacgacca acgaagaagt catcgctgta
    31561 tgccgtgtca ccgccaaact caggcagcat atcggtgtaa cccagtgcgt cgtaaaccac
    31621 accgtagtta cgaccgtagt cgatagaacc cgcatcagcg aatttcagac ctgcgaatgc
    31681 cagacgagtt ttgttacctt tctgggcatc agcgccttca gagttgttac cctggaagtt
    31741 atattcccac tgaccataac cggtcagatc ggaattgatt tgtgtttccc ctttaaaacc
    31801 aagacgggca taagttttgt cgccgtttcc gccgtaactg tttacaccat tgtctttaga
    31861 aaaataatgc agaccgacag ctttaccgta cagatctact ttgttgccat ctttgttata
    31921 gatttctgca gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat
    31981 attgcgcttc atcattattt attaccctca tggttt
//
LOCUS       DAZUCE010000029        31696 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZUCE010000029 DAZUCE010000000
VERSION     DAZUCE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.029"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(11..901)
                     /gene="rarD"
                     /locus_tag="ACHY03_001185"
     CDS             complement(11..901)
                     /gene="rarD"
                     /locus_tag="ACHY03_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFS3904444.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(953..1420)
                     /gene="yigI"
                     /locus_tag="ACHY03_001186"
     CDS             complement(953..1420)
                     /gene="yigI"
                     /locus_tag="ACHY03_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFS3904445.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1585..2454
                     /gene="pldA"
                     /locus_tag="ACHY03_001187"
     CDS             1585..2454
                     /gene="pldA"
                     /locus_tag="ACHY03_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFS3904446.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2587..4416
                     /gene="recQ"
                     /locus_tag="ACHY03_001188"
     CDS             2587..4416
                     /gene="recQ"
                     /locus_tag="ACHY03_001188"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFS3904447.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4480..5100
                     /gene="rhtC"
                     /locus_tag="ACHY03_001189"
     CDS             4480..5100
                     /gene="rhtC"
                     /locus_tag="ACHY03_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFS3904448.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWSGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFVLIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5162..5782)
                     /gene="rhtB"
                     /locus_tag="ACHY03_001190"
     CDS             complement(5162..5782)
                     /gene="rhtB"
                     /locus_tag="ACHY03_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFS3904449.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5893..6915
                     /gene="pldB"
                     /locus_tag="ACHY03_001191"
     CDS             5893..6915
                     /gene="pldB"
                     /locus_tag="ACHY03_001191"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFS3904450.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6923..7723
                     /gene="yigL"
                     /locus_tag="ACHY03_001192"
     CDS             6923..7723
                     /gene="yigL"
                     /locus_tag="ACHY03_001192"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFS3904451.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7799..8698
                     /gene="bioP"
                     /locus_tag="ACHY03_001193"
     CDS             7799..8698
                     /gene="bioP"
                     /locus_tag="ACHY03_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFS3904452.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8586..9539)
                     /gene="metR"
                     /locus_tag="ACHY03_001194"
     CDS             complement(8586..9539)
                     /gene="metR"
                     /locus_tag="ACHY03_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFS3904453.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSSMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9775..12036
                     /gene="metE"
                     /locus_tag="ACHY03_001195"
     CDS             9775..12036
                     /gene="metE"
                     /locus_tag="ACHY03_001195"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFS3904454.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12076..12891)
                     /gene="ysgA"
                     /locus_tag="ACHY03_001196"
     CDS             complement(12076..12891)
                     /gene="ysgA"
                     /locus_tag="ACHY03_001196"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFS3904455.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13153..13914
                     /gene="udp"
                     /locus_tag="ACHY03_001197"
     CDS             13153..13914
                     /gene="udp"
                     /locus_tag="ACHY03_001197"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFS3904456.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14055..15482
                     /gene="rmuC"
                     /locus_tag="ACHY03_001198"
     CDS             14055..15482
                     /gene="rmuC"
                     /locus_tag="ACHY03_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFS3904457.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15577..16332
                     /gene="ubiE"
                     /locus_tag="ACHY03_001199"
     CDS             15577..16332
                     /gene="ubiE"
                     /locus_tag="ACHY03_001199"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFS3904458.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16346..16951
                     /gene="ubiJ"
                     /locus_tag="ACHY03_001200"
     CDS             16346..16951
                     /gene="ubiJ"
                     /locus_tag="ACHY03_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFS3904459.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16948..18588
                     /gene="ubiB"
                     /locus_tag="ACHY03_001201"
     CDS             16948..18588
                     /gene="ubiB"
                     /locus_tag="ACHY03_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFS3904460.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18667..18936
                     /gene="tatA"
                     /locus_tag="ACHY03_001202"
     CDS             18667..18936
                     /gene="tatA"
                     /locus_tag="ACHY03_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFS3904461.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18940..19455
                     /gene="tatB"
                     /locus_tag="ACHY03_001203"
     CDS             18940..19455
                     /gene="tatB"
                     /locus_tag="ACHY03_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFS3904462.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19458..20234
                     /gene="tatC"
                     /locus_tag="ACHY03_001204"
     CDS             19458..20234
                     /gene="tatC"
                     /locus_tag="ACHY03_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFS3904463.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20276..21058
                     /gene="tatD"
                     /locus_tag="ACHY03_001205"
     CDS             20276..21058
                     /gene="tatD"
                     /locus_tag="ACHY03_001205"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFS3904464.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21055..21543)
                     /gene="rfaH"
                     /locus_tag="ACHY03_001206"
     CDS             complement(21055..21543)
                     /gene="rfaH"
                     /locus_tag="ACHY03_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFS3904465.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21710..23203
                     /gene="ubiD"
                     /locus_tag="ACHY03_001207"
     CDS             21710..23203
                     /gene="ubiD"
                     /locus_tag="ACHY03_001207"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFS3904466.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23249..23950
                     /gene="fre"
                     /locus_tag="ACHY03_001208"
     CDS             23249..23950
                     /gene="fre"
                     /locus_tag="ACHY03_001208"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFS3904467.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24142..25305)
                     /gene="fadA"
                     /locus_tag="ACHY03_001209"
     CDS             complement(24142..25305)
                     /gene="fadA"
                     /locus_tag="ACHY03_001209"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFS3904468.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25315..27504)
                     /gene="fadB"
                     /locus_tag="ACHY03_001210"
     CDS             complement(25315..27504)
                     /gene="fadB"
                     /locus_tag="ACHY03_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFS3904469.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVDDLLVEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27694..29025
                     /gene="pepQ"
                     /locus_tag="ACHY03_001211"
     CDS             27694..29025
                     /gene="pepQ"
                     /locus_tag="ACHY03_001211"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFS3904470.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29025..29639
                     /gene="yigZ"
                     /locus_tag="ACHY03_001212"
     CDS             29025..29639
                     /gene="yigZ"
                     /locus_tag="ACHY03_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFS3904471.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29678..31129
                     /gene="trkH"
                     /locus_tag="ACHY03_001213"
     CDS             29678..31129
                     /gene="trkH"
                     /locus_tag="ACHY03_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFS3904472.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31141..31686
                     /gene="hemG"
                     /locus_tag="ACHY03_001214"
     CDS             31141..31686
                     /gene="hemG"
                     /locus_tag="ACHY03_001214"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFS3904473.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7325 a         8091 c         8688 g         7592 t
ORIGIN      
        1 tatttatttt ttatttggac gttctacgct gcgtataaat cgcatccatc acaaaaattg
       61 ccagcgccac ccaaataaag gcgaaagtca ccatcttatc ggcacccggt ttttcaccat
      121 aaaacgtcac agccagcagg aacatcagcg tcgggccaat gtactggaaa aagcctaacg
      181 ttgagagacg caggcgcgtg gcagcggcag taaaacacaa cagcggtacg gtagtgacaa
      241 taccggcggc gatcagcagt aaattcagcg acatcgggtt ttgccccata tggctggttg
      301 agctgtcggc aatagcaaac aggtaaatcg ccgccacggg cagcagccac atggtttcaa
      361 ttaacatgcc ggtttgtgct tctaccgcaa tcttcttgcg caccaggccg tagaaggcaa
      421 aactaaatgc cagccccagc gcgataatag gtagcgaacc aaaagtccac agctggacta
      481 acacgccaca tatcgccaga atcaccgcca gccattgcat ccggcggaat cgctcgccga
      541 ggaaaatcat ccccagcaca atgttcacca gcgggttaat aaagtaacca aggctcgctt
      601 ccagcatatg gtgattgttc accgcccaga taaacagtag ccagttgccg ccaatcagca
      661 cggcagagac tgccagcata aaaattttct gtggcgtctg aatcagcgtt tttaaatagg
      721 accactggcg gcaaatgctc atcagcacca ccataaagaa aaacgaccag atcacgcgat
      781 gcgtcaggat ttcatcggcg ggcacgtagt aaatcaactt gaagtacgct ggggctatac
      841 cccaaataaa ataagcggca agagcgagta atacgccctg ccgcgtttgt tttgcatcca
      901 tcgggaatac tcatttttaa ttggtaacag cagtttacct gcttttatgt cttcaaccta
      961 ccatataggt ggcggtggca ctggcaatat aaagctgttc ttcattgtgt aattcaacgc
     1021 gggcgacggc gactttattg cctgcacgca acagactact ggtagcagta aaacgctcgc
     1081 ccctgcctgg gcgcagataa tcaacgcgaa gatcaatggt ccccatccgc gatagccgct
     1141 ggcgtagttc atcttcactg atggtttcgt ggcgggttaa ggtacttcct acgcacacca
     1201 gaccggcggc gacatccagc gccgacgcaa tgaccccgcc gtgcaaaatg ctttgcgccc
     1261 agttgcccac catcattggc tgatttttaa aggccagctg tgcgaactct ttttcgtaac
     1321 gctccagttc catccccaat gcgcggttaa atggcatgtg ataaacaaac atctcaccca
     1381 ctaatttcag ggcttgttca gcggtcagta cggcagacat atcatcctta cacttcattg
     1441 gttaatgaaa tgttgatttt atgcttcttt gttgttggtt tctactttag gaagggataa
     1501 ctaacggcta tggagttaag tatgtaaaat agcccgcaga aaaatattca ccttatcaat
     1561 aattcgttac ggagaacacg accgatgcgg actctgcagg gctggttgtt gccggtgttt
     1621 atgttgccta tggcagtata tgcacaagag gcaacggtga aagaggtgca tgacgcgcca
     1681 gcggtgcgtg gcagtattat cgccaatatg ctgcaggagc atgacaatcc gttcacgctc
     1741 tatccttatg acaccaacta cctcatctac acccaaacca gcgatctgaa taaagaagcg
     1801 attgccagtt acgactgggc ggaaaatgcg cgtaaggatg aagtaaagtt tcagttgagc
     1861 ctggcatttc cgctgtggcg tgggatttta ggcccgaact cggtgttggg tgcgtcttat
     1921 acgcaaaaat cctggtggca actgtccaat agcgaagagt cttcaccgtt tcgtgaaacc
     1981 aactacgaac cgcaattgtt cctcggtttt gccaccgatt accgttttgc aggttggacg
     2041 ctgcgcgatg tggagatggg gtataaccac gactctaacg ggcgttcaga cccgacctcc
     2101 cgcagctgga accgccttta tactcgcctg atggcagaaa acggtaactg gctggtagaa
     2161 gtgaagccgt ggtatgtggt gggtaatact gacgataacc cggatatcac caaatatatg
     2221 ggttactacc agcttaaaat cggctatcac ctcggtgatg cggtgctcag tgcgaaagga
     2281 cagtacaact ggaacaccgg ctacggcggc gcggagttag gcttaagtta cccgatcacc
     2341 aaacatgtgc gcctttatac tcaggtttac agcggctatg gcgaatcgct catcgactat
     2401 aacttcaacc agacccgtgt cggtgtgggg gttatgctaa acgatttgtt ttgatgaacg
     2461 gttgagtggt tggcaaatct ggaatccagc atccaggatt accctctcag agactaaaag
     2521 cattgcagtt tctcgcgcag gcgctgaaaa tagcgcctgt ttttatttca ggcaatcggg
     2581 gtgaatgtgg cgcaggcgga agtgttgaat ctggagtccg gagctaaaca ggttttacaa
     2641 gaaacctttg gctaccaaca gtttcgcccc ggccaggaag aaattatcga cactgtgctt
     2701 tccggtcgcg attgcctggt cgtcatgccc accggtggcg gaaaatccct ttgctatcaa
     2761 attcctgcct tattgctaaa cggccttacc gtggttgttt caccgctgat ttcgttgatg
     2821 aaagatcagg tggatcaact gcaagccaac ggcgtggcgg cggcgtgcct taactcgacg
     2881 caaacccgcg aacagcaact tgaagtgatg acaggctgcc gcaccgggca aattcgtctg
     2941 ctttatatcg ccccggaacg cctgatgctg gataactttc ttgagcatct ggcgcactgg
     3001 aatccggtgt tattagccgt tgatgaagcg cactgtatct cccaatgggg ccacgatttc
     3061 cgcccggaat atgccgcgct cggtcagttg cgccagcggt tcccgacgct gccgtttatg
     3121 gcgctgaccg ccacagccga cgacaccacg cgccaggata tcgtgcgcct gctggggctg
     3181 aacgatccgc tgattcaaat cagcagtttt gaccgtccga atattcgcta catgctgatg
     3241 gagaaattca aaccgctcga tcagttgatg cgctacgtgc aggaacagcg cggtaagtcc
     3301 ggcattatct actgcaacag ccgggcgaaa gtagaagaca ccgctgcgcg cctgcaaagc
     3361 aagggtatta gcgcggcggc ctatcatgcc gggctggaaa ataatgtccg cgccgacgtg
     3421 caggagaaat tccagcgcga tgacctgcaa attgtggtgg cgacggtggc gttcggcatg
     3481 ggcatcaata aaccaaacgt tcgcttcgtg gtccactttg atattccgcg caatatcgaa
     3541 tcctattatc aggaaaccgg tcgcgccggg cgtgatggcc tgcctgcgga agcgatgctg
     3601 ttttacgatc cggctgatat ggcgtggctg cgccgttgtc tggaagagaa gccgcagggg
     3661 cagttgcagg atatcgagcg ccacaaactc aatgcgatgg gcgcgtttgc cgaagcgcaa
     3721 acttgccgtc gtctggtgct gctgaactac tttggcgaag ggcggcagga gccgtgcggg
     3781 aactgcgata tctgcctcga tccgccgaaa caatacgatg gttcaaccga tgctcagatt
     3841 gccctttcca ccattggtcg tgtgaatcag cggtttggga tgggttatgt ggtggaagtg
     3901 attcgtggtg ctaataacca gcgtatccgc gactatggtc atgacaaact gaaagtctat
     3961 ggcatgggcc gtgataaaag ccatgaacat tgggtgagcg tgatccgcca gctgattcac
     4021 ctcggcctgg tgacgcaaaa tattgcccag cattctgccc tacaactgac agaggctgcg
     4081 cgcccggtgc tgcgcggcga atcctctttg caacttgccg tgccgcgtat cgtggcgctc
     4141 aaaccgaaag cgatgcagaa atcgttcggc ggcaactatg atcgcaaact gttcgccaaa
     4201 ttacgcaaac tgcgtaaatc gattgccgat gaaagtaatg tcccgccgta cgtggtgttt
     4261 aacgacgcaa ccttgattga gatggctgaa cagatgccga tcaccgccag cgaaatgctc
     4321 agcgttaacg gcgttgggat gcgcaagctg gaacgctttg gtaaaccgtt tatggcgctg
     4381 attcgtgcgc atgtcgatgg cgacgacgaa gagtagtcag cagcataaaa aagtgccagg
     4441 atgaagactc cgtaaacgtt tcccccgcga gtcaaatgta tgttgatgtt atttctcacc
     4501 gtcgccatgg tgcacattgt ggcgcttatg agtcccggtc ccgatttctt ttttgtctct
     4561 cagaccgctg tcagtcgttc ccgtaaagaa gcgatgatgg gcgtgctcgg tattacctgc
     4621 ggcgttatgg tttggtcagg gattgcgttg cttggcctgc atttgattat cgaaaaaatg
     4681 gcctggctgc atacgctgat tatggttggc ggtggcctgt atctctgctg gatgggttac
     4741 cagatgctgc gcggcgcgct gaaaaaagag gcggtttctg cacctgcgcc acaggtcgag
     4801 ctggcgaaaa gtgggcgcag tttcctgaaa ggtttactga ccaatctcgc taatccgaaa
     4861 gcgattatct actttggctc ggtgttctca ttgtttgtcg gtgataacgt tggcactacc
     4921 gcgcgctggg gcatttttgt gctgatcatt gtcgaaacac tggcgtggtt taccgtcgtt
     4981 gccagcctgt ttgccctgcc gcaaatgcgc cgtggttatc aacgtctggc gaagtggatt
     5041 gatggttttg ccggggcgtt atttgccgga tttggcattc atttgattat ttcgcggtga
     5101 tgccagacgc gtcttcagag taagtcgaat aaggcgttta cgccgcatcc gacattattt
     5161 ttcacgcatg cctcgccgat gctaacagcg ctcccaccag cataaacaac gagccgaaaa
     5221 tcttattcag cgccttcatc tgctttggtc ctttaatcca tagagcaatc cgttgagcaa
     5281 gggtggcgta accgatcatc acaataatat cgaccacaat agtggtgacg ccgagcacga
     5341 tatactgcat cagttgcggc tgttgcggca tgatgaattg cggaaatagc gccgccagaa
     5401 acacaatact tttgggattg gtgagattca caaaaactgc gcgctggaac aaatgtcgac
     5461 gcgattgagt agaggccagc gatttaagat caattgcgcc agcggcgcgc cattgctgta
     5521 ttcccagcca aatcaagtaa gccgcgcctg cccacttcaa cacttcaaac gcaatcactg
     5581 agcgggaaaa tagcgtcccc aaccccacgc caaccagcac aatatgaatc gccagtccgg
     5641 tctgaagccc agcaatagac gccaccgcgc cgcgataacc gtggttgagc gaggtggtca
     5701 tagtgttgat tgcaccagag cctggcgaca ggcttaaaat gatcgatgtc agcaggtagg
     5761 caaaccacca ttctaaggtc atgatgaact cccggtgtgt ctatttttgt gccacaatac
     5821 gctactgtcg cagcgttgtg tcaggcacgc taaaaaaaac gattttacgt ggtttaagag
     5881 gcagattacc cgatgtttca gcagcaaaaa gactgggaaa caagagaaaa cgcgtttgct
     5941 gcttttacca tgggaccgct gactgatttc tggcgtcagc gtgatgaagc agagtttact
     6001 ggtgtggatg acattccggt gcgctttgtc cgttttcgcg cacagcacca tgaccgggtg
     6061 gtagtcatct gcccggggcg tattgagagc tacgtaaaat atgcggaact ggcctacgat
     6121 ttgttccatt tggggtttga tgtcttaatc atcgaccatc gcgggcaggg acgttccggt
     6181 cgcctgttag ccgatccgca tctcgggcat gttaatcgct ttaatgatta tgttgatgat
     6241 ctggcggcat tctggcaaca ggaggtccag cccggtccgt ggcgtaaacg ctatatactg
     6301 gcacattcga tgggcggtgc gatctccaca ttatttctgc aacgccatcc aggtgtatgt
     6361 gacgccattg cgctaactgc gccaatgttt gggatcgtga ttcgtatgcc gtcatttatg
     6421 gcacggcaga tcctcaactg ggccgaagcg catccacgtt tccgtgatgg ctatgcaata
     6481 ggcaccgggc gctggcgcgc gttgccgttt gctatcaacg tactgaccca cagcagacag
     6541 cgatatcgac gtaacttacg cttctatgct gatgacccaa cgattcgcgt cggtgggccg
     6601 acctaccatt gggtacgcga aagtattctg gctggcgaac aggtgttagc cggtgcgggt
     6661 gatgacgcca cgccaacgct tctcttacag gctgaagagg aacgcgtggt ggataaccgc
     6721 atgcatgacc gtttttgtga actccgcacc gccgcgggcc atcctgtcga aggaggacgg
     6781 ccgttggtaa ttaaaggtgc ttaccatgag atcctttttg aaaaggacgc aatgcgctca
     6841 gtcgcgctcc acgccatcgt tgattttttc aacaggcata actcacccag cggaaaccgc
     6901 tctacagagg tttaaatttc ttatgtacca ggttgttgcg tctgatttag atggcacgtt
     6961 actttctccc gaccatacgt tatcccctta cgccaaagaa accctgaagc tgctcaccgc
     7021 gcgcggcatc aactttgtgt ttgcgaccgg tcgtcaccac gttgatgtgg ggcaaattcg
     7081 cgataatctg gagattaagt cttacatgat tacctccaat ggtgcgcgcg ttcacgatct
     7141 ggatggtaat ctgatttttg ctcataacct ggatcgcgac attgccagcg atctgtttgg
     7201 cgtagtcaac gacaatccgg acatcattac taacgtttat cgcgacgacg aatggtttat
     7261 gaatcgccat cgcccggaag agatgcgctt ttttaaagaa gcggtgttca aatatgcgct
     7321 gtatgagcct ggattactgg agccggaagg tgtcagcaaa gtgttcttca cctgcgattc
     7381 ccatgaacaa ctgctgccgc tggagcaggc gattaacgct cgttggggcg atcgcgtcaa
     7441 cgtcagtttc tctaccttaa cctgtctgga agtgatggcg ggcggcgttt caaaaggcca
     7501 tgcgctggaa gcggtggcga agaaactggg ctacagcctg aaggattgta ttgcgtttgg
     7561 tgacgggatg aacgatgccg aaatgctgtc gatggcgggg aaaggctgca ttatgggcag
     7621 tgcgcaccag cgtctgaaag accttcatcc cgagctggaa gtgattggta ctaatgccga
     7681 cgacgcggtg ccgcattatc tgcgtaaact ctatttatcg taatcgttct ttatttggtc
     7741 agttgtcaac ctgattcttc gctacaatgg atactcgtta atcaaagagt tttccattgt
     7801 ggcgctactt atcatcacca cgattctgtg ggccttctcc tttagctttt atggcgagta
     7861 ccttgcgggg cacgtcgata gctattttgc ggtgctggtg cgcgttggcc tggcggcact
     7921 cgtttttctg ccgtttctgc gtacccgtgg caatagcctg aaaacagtcg gcctgtatat
     7981 gctggtgggc gcgatgcagc tcggcgtgat gtatatgctg agtttccgcg cttatctcta
     8041 cctgacggtt tccgaactgc tgctgttcac cgtactgacg ccgctctaca tcacgctgat
     8101 ttatgacatc atgagtaagc gccgtctgcg ctggggatat gcttttagtg ccttgttggc
     8161 ggtgattggt gccgggatta ttcgctatga tcaggtcacc gaccatttct ggactggctt
     8221 gttgctggtg caactctcca atatcacttt tgccatcggc atggtgggtt acaaacgcct
     8281 gatggaaact cgcccgatgc cgcagcataa cgcctttgcg tggttctatc ttggcgcgtt
     8341 cctggtggcg gtaattgcat ggttcttgct gggaaatgcg cagaaaatgc cgcaaaccac
     8401 gttgcaatgg ggcattctgg tgtttcttgg cgtggtggct tccgggattg gctactttat
     8461 gtggaactac ggcgcgacgc aggtggacgc cggaacgctg ggaattatga ataatatgca
     8521 cgttccggca gggctgctgg ttaacctggc tatctggcac caacagccgc actggccaac
     8581 gtttattaca ggcgcgctgg tgatcctggc ctcactgtgg gtgcatcgta agtgggtcgc
     8641 tccgcgctct tcacaaacgg cagatgatcg caggcgtgat tgcgcgctga gcgaataaac
     8701 gcttccgtaa ctggctgacg ctgctcgcca tcgcgcacgg cggcgtacag tcggctccac
     8761 aagccttcgc ccagggtttt ggtcaccacc agaccctggc gctcaaaact ctctactacc
     8821 caatgcggca gggcggcaat acccatccgc gcggcaacca tctgaatcaa caataaggtg
     8881 ttatcgacgc ttttcagtga cgggctgacg cctgccggct gaagaaaatg ccgccagaca
     8941 tccagtcggc tacgctgcac cggataaatc aacagcgtct cgctggcgag atcttccggt
     9001 gtaatacgcg tttttgccgc caatggatgg tcaggtgcta acaccaggcg tacttcatag
     9061 tcgaacatcg acgaataatg caggccactg cgcggcagaa tatcggaggt catcaccaga
     9121 tccagctcgc cctgttgcaa cgcgggctgc gggtcaaacg tcacgcccga tttaaaatcc
     9181 atctctacct gcggccagtt cttatggaaa ttttctaacg cgggtgtcag ccactgaata
     9241 cagctatggc actcaatggc aatgcgcaga cgcgtctgct gcggttcatt gcaggcttgc
     9301 agtgcctggc taatttgcgg cagcacctgg tttgccagtt gcaacaggat ttctccctgc
     9361 ggtgtaaagc gtagcggctg gctcttacgc acaaatagcc ggaagccaag gcgttgttcc
     9421 agatcgctaa actggtgaga cagggcggat tgcgtctgat gcaacgtcgc cgcagcggct
     9481 gcgagagagc cgcagttccg caacgcttgt agcgttttca ggtgttttac ttcgatcatg
     9541 aaagtccttc acttcggcat gaataatttg cgcttgagga atatacagta accgccaatt
     9601 atggatgtgt aaacatctgg acggctaaaa tccttcgtct tttaaattta tggtgcgttg
     9661 gctgcgtttc tccaccccgg tcacttactt cagtaagctc ccggggatga ataaacttgc
     9721 cgccttccct aaattcaaaa tccataggat ttacatataa ttagaggaag aaaaatgaca
     9781 atattgaatc acaccctcgg tttccctcgc gttggcctgc gtcgcgagct gaaaaaagcg
     9841 caagagagtt attgggcggg gaactccacg cgtgaagaac tgctggcggt agggcgtgaa
     9901 ttgcgtgctc gtcactggga tcaacaaaag caagcgggta tcgacctgct gccggtgggc
     9961 gattttgcct ggtacgatca tgtactgacc accagtctgc tgctgggtaa cgttccggcg
    10021 cgtcatcaga acaaagacgg ttcggtagat atcgacaccc tgttccgtat tggtcgtgga
    10081 cgtgcgccga ctggcgaacc tgcggcggca gcggaaatga ccaaatggtt taacaccaac
    10141 tatcactaca tggtgccgga attcgttaaa ggccaacagt tcaaactgac ctggacgcag
    10201 ctgctggagg aagtggacga ggcgctggcg ctgggccaca acgttaagcc tgtgctgctg
    10261 gggccggtta cctggctgtg gctggggaaa gtgaaaggtg aacagtttga ccgccttagc
    10321 ctgctgaacg acattctgcc ggtttatcag caagtgctgg cagaactggc gaaacgcggc
    10381 atcgagtggg tacagattga tgaacccgcg ctggtactgg aactaccaca ggcgtggctg
    10441 gacgcataca aacccgctta cgacgcgctc cagggacagg tgaaactgct gctgaccacc
    10501 tattttgaag gcgtaacgcc aaatctcgac acgattactg cgctgcctgt tcagggtctg
    10561 catgttgacc tcgtacatgg caaagatgac gttgccgaac tgcacaagcg tctaccttct
    10621 gactggctac tgtctgctgg tcttatcaat ggtcgtaacg tctggcgcgc cgatcttact
    10681 gagaaatatg cgcaaattaa ggacattgtc ggcaaacgtg atttgtgggt ggcatcttcc
    10741 tgctcgttgc tgcacagccc catcgacctg agcgtggaaa cgcgtcttga tgcagaagtg
    10801 aaaagctggt ttgccttcgc cctacaaaaa tgccatgaac tggcactgct gcgcgatgcg
    10861 ctgaacagtg gtgacacggc agctctggca gagtggagcg ccccaattca ggcgcgtcgt
    10921 cactctactc gtgtacataa tccggcggta gaaaagcgtc tggcggcgat caccgcccag
    10981 gacagccagc gtgcgaatgt ctatgaagtg cgtgctgaag cccagcgtgc gcgttttaaa
    11041 ctgccagcgt ggccgaccac cacgattggt tccttcccgc aaaccacgga aattcgtacc
    11101 ctgcgtctgg atttcaaaaa gggcaatctc gacgccaaca actaccgcac gggtattgcg
    11161 gaacatatca ggcaggccat tgttgagcag gagcgtttag gtctggatgt gctggtacat
    11221 ggcgaggccg agcgtaatga catggtggaa tactttggcg agcacctcga cggttttgtc
    11281 tttacgcaaa acggttgggt acagagctac ggttcccgct gcgtgaagcc accgattgtt
    11341 attggtgacg ttagtcgccc ggcaccgatt accgtggagt gggcgaagta tgcgcaatct
    11401 ctgactgata aaccggtgaa agggatgctg actggcccgg tgaccattct ctgctggtcg
    11461 ttcccgcgtg aagatgtcag ccgtgaaacc atcgccaaac aaattgcgct ggcgctgcgt
    11521 gatgaagtgg cggacctgga agccgcagga attggcatca tccagattga tgaaccggcg
    11581 ctgcgcgaag gtttaccgct gcgtcgtagc gactgggatg catatctcca gtggggcgtg
    11641 gaggccttcc gtatcaacgc cgccgtggcg aaagatgaca cacaaatcca cactcacatg
    11701 tgttattgcg agttcaacga catcatggat tcgattgcgg cactggacgc agacgtcatc
    11761 accatcgaaa cctcgcgttc tgacatggag ttgctggagt cgttcgaaga gtttgattat
    11821 ccgaatgaaa tcggtccagg cgtctatgac attcactcgc caaacgtacc gagcgtagaa
    11881 tggattgaag ccttgctgaa gaaagcagca aaacgcattc cggcagagcg cctgtgggtt
    11941 aacccggact gtggcctgaa aacgcgcggc tggccagaaa cccgcgcggc actggcgaac
    12001 atggtgcagg cggcgcagaa cttgcgtcgg gggtaaaatc caaaccgggt ggtaatacca
    12061 cccggtcttt tctcattaca gcgacttctt cccaccatac tgcttaaacc attccagcat
    12121 acgctgccag ccatcttctg cagatgcggc atgatagctc gggcgataat cagcgttgaa
    12181 tgcatgcccg gcgtccgggt acacgataat ctctgctttc gcattagcag cccgcagcgc
    12241 ctggcgcatg gtttcaacgc tctcctgcgg aatactgtta tccagaccgc catataagcc
    12301 gagaaccggc gcgttaagat cggttgcgat atcaacaggt tgtttcggtg aattcagcga
    12361 cttatcgccc gtcagtttgc cgtaccacgc cactgcggct tttagctgtg gattatgcgc
    12421 agcatacagc caggtgatac gtccacccca gcagaatccg gtgatcatta aacgatgaac
    12481 atcgccgccg ttgcgggaag cccaactggc gacatgatcg agatcggcca gcacctgcga
    12541 gtcaggcact tttgctacca gaccgctaag caacgtgggg atatcggcaa aatcattcgg
    12601 atcgccttcg cggaagtaaa gttcaggtgc gatagccaga tacccctcca gcgccagacg
    12661 gcgacaaatg tcgcggatat gttcatgcac gccaaaaatt tcctgcacta caatgaccac
    12721 tggcagtggg ccatcgcttt gctttggtct ggcatggtaa gcaggcatgt tatccccttg
    12781 tgaagggata gaggtgaagc ccgccacaat cgcgtcgtcc ggggtctgaa cgatggtcga
    12841 agcgagaggc gatgcagcag gtgcaaatcc agattgttgt gttgttgcca tggtattctc
    12901 cgtaccctta taaaaatgtt gcgcaatgtt aactatagtc agcatgcaac aaatcacatt
    12961 gcctgaatcg gctcatcttt tatgcagtcc tgcagaatga agagtgattt atgtgatttg
    13021 catcactttt ggtgggtaaa tttatgcaac gcatttgcgt catggtgatg agtatcacga
    13081 aaaaatgtta aacccttcgg taaagtgtct ttttgcttct tctgactaaa ccgattcaca
    13141 gaggagttgt atatgtccaa gtctgatgtt tttcatctcg gcctcactaa aaacgattta
    13201 caaggggcta cgcttgccat cgtccctggc gacccggatc gtgtggaaaa gatcgccgcg
    13261 ctgatggata agccggttaa gctggcatct caccgcgaat tcaccacctg gcgtgcagag
    13321 ctggatggta aacctgttat cgtctgctct accggtatcg gcggcccgtc tacctctatt
    13381 gctgttgaag agctggcaca gctgggcatt cgcaccttcc tgcgtatcgg tacaacgggc
    13441 gctattcagc cgcatattaa tgtgggtgat gtcctggtta ccacggcgtc tgtccgtctg
    13501 gatggcgcga gcctgcactt cgcaccgctg gaattcccgg ctgtcgctga tttcgaatgt
    13561 acgactgcgc tggttgaagc tgcgaaatct attggcgcga caactcacgt tggcgtgaca
    13621 gcttcttctg ataccttcta cccaggtcag gaacgttacg atacttactc tggtcgcgta
    13681 gttcgtcact ttaaaggttc tatggaagag tggcaggcga tgggcgtaat gaactatgaa
    13741 atggaatctg caaccctgct gaccatgtgt gcaagtcagg gcctgcgtgc cggtatggta
    13801 gcgggtgtta tcgttaaccg cactcagcaa gagatcccga atgctgagac gatgaaacaa
    13861 actgaaagcc aagcggtgaa aatcgtggtg gaagcggcgc gtcgtctgct gtaattctct
    13921 tctcctgtct gaaggccgac gcgttcggcc ttttgtattt ttgcgtagcg cctcgcagga
    13981 aatgcctttc caactggacg tttgtacagc acaattctat tttgtgcggg taagttgttg
    14041 cgtcaggagg cgttgtggat ttctcaatca tggtttacgc agttattgcg ttggtgggtg
    14101 tggcaattgg ctggctgttt gccagttatc aacatgcgca gcaaaaagcc gagcaattag
    14161 ctgaacgtga agagatggtc gcggagttaa gcgcggcaaa acaacaaatt acccaaagcg
    14221 agcactggcg tgcagagtgc gagttactca ataacgaagt gcgcagcctg caaagtatta
    14281 acacctctct ggaggccgat ctgcgtgaag taaccacgcg gatggaagcc gcacagcaac
    14341 atgctgacga taaaattcgc cagatgatta acagcgagca gcgcctcagt gagcagtttg
    14401 aaaacctcgc caaccgtatt tttgagcaca gcaatcgccg ggttgatgag caaaaccgtc
    14461 agagtctgaa cagcctgttg tcgccgctac gtgaacaact ggacggtttc cgccgtcagg
    14521 ttcaggacag cttcggtaaa gaagcacaag aacgccatac cctgacccac gaaattcgca
    14581 atctccagca actcaacgcg caaatggccc aggaagcgat caacctgacg cgcgcgctga
    14641 aaggcgacaa taaaacccag ggcaactggg gcgaggtagt attgacgcgg gtgctggagg
    14701 cttccggtct gcgtgaaggg tatgaatatg aaacccaggt cagcatcgaa aatgacgccc
    14761 gctcgcggat gcagccggat gtcatcgtgc ggctgccgca gggaaaagat gtggtgatcg
    14821 acgccaaaat gacgctggtc gcctatgaac gctattttaa cgccgaagac gactacaccc
    14881 gcgaaagcgc gctacaggaa catatcgcgt cggtgcgtaa ccatatccgt ttgctgggac
    14941 gcaaagatta tcaacagctg ccggggctgc gaactctgga ttacgtgctg atgtttattc
    15001 ccgttgaacc cgctttttta ctggcgcttg accgccagcc ggagctgatc accgaagcgt
    15061 tgaaaaacaa tatcatgctg gttagcccga ctacgctgct ggtggcgctg cgcactatcg
    15121 ccaacctgtg gcgttatgag catcaaagcc gcaacgccca gcaaatcgcc gatcgtgcca
    15181 gcaagctgta cgacaagatg cgtttgttca tcgatgacat gtcagcgatt ggtcaaagtc
    15241 tcgacaaagc gcaggataac taccgccagg caatgaaaaa actctcttca ggtcgcggaa
    15301 atgtgctggc gcaggcagaa gcgtttcgcg gtttaggagt agaaattaaa cgcgagatta
    15361 atccggattt ggctgaacag gcggtgagcc aggatgaaga gtatcgactt cggtcggttc
    15421 cggagcagcc gaatgatgaa gcttatcaac gcgatgatga atataatcag cagtcgcgct
    15481 agcccattgg gagtagttaa gccgggtaga aatctagggc atcgacgccc aatctgttac
    15541 acttctggaa caattttttg atgagcaggc attgagatgg tggataagtc acaagaaacg
    15601 acgcactttg gttttcagac cgtcgcgaag gaacaaaaag cggatatggt cgcccacgtt
    15661 ttccattccg tggcatcaaa atacgatgtc atgaatgatt tgatgtcatt tggtattcat
    15721 cgtttgtgga agcgattcac gattgattgc agcggcgtac gccgtgggca gaccgtgctg
    15781 gatctggctg gtggcaccgg cgacctgacg gcgaaattct cccgcctggt cggagaaact
    15841 ggcaaagtgg tccttgctga tatcaatgaa tccatgctca aaatgggccg cgagaagctg
    15901 cgtaatatcg gtgtgattgg caacgttgag tatgttcagg cgaacgctga ggcgctgccg
    15961 ttcccggata acacctttga ttgcatcacc atttcgtttg gtctgcgtaa cgtcaccgac
    16021 aaagataaag cactgcgttc aatgtatcgc gtgctgaaac ccggcggccg cctgctggtg
    16081 cttgagttct cgaagccaat tatcgagccg ctgagcaaag cctatgatgc atactccttc
    16141 catgtgctgc cgcgtattgg ctcactggtc gcgaacgacg ccgacagcta ccgttatctg
    16201 gcagaatcca tccgtatgca tcccgatcag gataccctga aagccatgat gcaggatgcc
    16261 ggattcgaaa gtgtcgacta ctacaatctg acggcagggg ttgtggcgct gcatcgtggt
    16321 tataagttct gacaggagac cggaaatgcc ttttaaacct ttagtgacgg caggaattga
    16381 aagtctgctc aacaccttcc tgtatcgctc acccgcgctg aaaacggccc gctcgcgtct
    16441 gttgggcaaa gtgctgcgcg tggaggtaaa aggcttttcg acgtcattga ttctggtgtt
    16501 cagcgaacgc caggttgatg tactgggcga atgggcaggc gatgctgact gcaccgttat
    16561 cgcctacgcc agtgtgttgc cgaaacttcg cgatcgccag cagcttgccg ccctgattcg
    16621 cagtggtgag ctggaagtgc agggcgatat tcaggtggtg caaaacttcg ttgcgctggc
    16681 agatctggca gagttcgacc ctgcggaact gctggcccct tataccggtg atatcgccgc
    16741 tgaaggaatc agcaaagcca tgcgcggagg cgcaaagttc ctgcatcacg gcattaagcg
    16801 ccagcaacgt tatgtggcgg aagccattac tgaagagtgg cgtatggcac ccggtccgct
    16861 tgaagtggcc tggtttgcgg aagagacggc tgccgtcgag cgtgctgttg atgccctgac
    16921 caaacggctg gaaaaactgg aggctaaatg acgccaggtg aagtacggcg cctatatttc
    16981 atcattcgca cttttttaag ctacggactt gatgaactga tcccaaaaat gcgtatcacc
    17041 ctgccgctac ggctatggcg atactcatta ttctggatgc caaatcggca taaagacaaa
    17101 cttttaggtg agcgactacg actggccctg caagaactgg ggccggtatg gatcaagttc
    17161 gggcaaatgt tatcaacccg ccgcgatctt tttccgccgc atattgccga tcagctggcg
    17221 ttattgcagg acaaagttgc tccgtttgat ggcaagctgg cgaagcagca gattgaagct
    17281 gcaatgggcg gcttgccggt agaagcgtgg tttgacgatt ttgaaatcaa gccgctggct
    17341 tctgcttcta tcgcccaggt tcataccgcg cgattgaaat cgaatggtaa agaggtggtg
    17401 attaaagtca tccgcccgga tattttgccg gttattaaag cggatctgaa acttatctac
    17461 cgtctggctc gctgggtgcc gcgtttgctg ccggatggtc gccgtctgcg cccaaccgaa
    17521 gtagtgcgcg agtacgaaaa gacattgatt gatgaactga atttgctgcg ggaatctgcc
    17581 aacgccattc agcttcggcg caattttgaa gacagcccga tgctctacat cccggaagtt
    17641 taccctgact attgtagtga agggatgatg gtgatggagc gcatttacgg cattccggtg
    17701 tctgatgttg cggcgctgga gaaaaacggc accaacatga aattgctggc ggaacgcggc
    17761 gtgcaggtgt tcttcactca ggtctttcgc gacagctttt tccatgccga tatgcaccct
    17821 ggcaacatct tcgtaagcta tgaacacccg gaaaacccga aatatatcgg cattgattgc
    17881 gggattgttg gctcgctaaa caaagaagat aaacgctatc tggcagaaaa ctttatcgcc
    17941 ttctttaatc gcgactatcg caaagtggca gagctacacg tcgattctgg ctgggtgcca
    18001 ccagatacca acgttgaaga gttcgaattt gctattcgta cggtctgtga acctatcttt
    18061 gagaaaccgc tggccgaaat ttcgtttgga catgtactgt taaatctgtt taatacggcg
    18121 cgtcgcttca atatggaagt gcagccgcaa ctggtgttac tccagaaaac cctgctctac
    18181 gtcgaagggg taggacgcca gctttatccg caactcgatt tatggaaaac ggcgaagcct
    18241 ttcctggagt cgtggattaa agatcaggtc ggtattcctg cgctggtgag agcatttaaa
    18301 gaaaaagcgc cgttctgggt cgaaaaaatg ccagaactgc ctgaattggt ttacgacagt
    18361 ttgcgccagg gcaagtattt acagcacagt gttgataaga ttgcccgcga gcttcagtca
    18421 aatcatgtac gtcagggaca atcgcgttat tttctcggaa ttggcgctac gttagtatta
    18481 agtggcacat tcttgttggt cagccgacct gaatgggggc tgatgcccgg ctggttaatg
    18541 gcaggtggtc tgatcgcctg gtttgtcggt tggcgcaaaa cacgctgatt ttttcatcgc
    18601 tcaaggcggg ccgtgtaacg tataatgcgg ctttgtttaa tcatcatcta ccacagagga
    18661 acatgtatgg gtggtatcag tatttggcag ttattgatta ttgccgtcat cgttgtactg
    18721 ctttttggca ccaaaaagct cggctccatc ggttccgatc ttggtgcgtc gatcaaaggc
    18781 tttaaaaaag caatgagcga tgatgaacca aagcaggata aaaccagtca ggatgctgat
    18841 tttactgcga aaactatcgc cgataagcag gcggatacga atcaggaaca ggctaaaaca
    18901 gaagacgcga agcgccacga taaagagcag gtgtaatccg tgtttgatat cggttttagc
    18961 gaactgctat tggtgttcat catcggcctc gtcgttctgg ggccgcaacg actgcctgtg
    19021 gcggtaaaaa cggtagcggg ctggattcgc gcgttgcgtt cactggcgac aacggtgcag
    19081 aacgaactga cccaggagtt aaaactccag gagtttcagg atagtctgaa aaaggttgaa
    19141 aaggcgagcc tcactaacct gacgcccgaa ctgaaagcgt cgatggatga attacgccag
    19201 gccgcggagt cgatgaagcg ttcctacgtt gcaaacgatc ctgaaaaggc gagcgatgaa
    19261 gcgcacacca tccataaccc ggtggtgaaa gacaatgaag ctgcgcatga gggcgtaacg
    19321 cctgccgctg cacaaacgca ggccagttcg ccggaacaga agccagaaac cacgccagag
    19381 ccggtggtaa aacctgctgc ggacgctgaa ccgaaaaccg ctgcaccttc cccttcgtcg
    19441 agtgataaac cgtaaacatg tctgtagaag atactcaacc gcttatcacg catctgattg
    19501 agctgcgtaa gcgtctgctg aactgcatta tcgcggtgat cgtgatattc ctgtgtctgg
    19561 tctatttcgc caatgacatc tatcacctgg tatccgcgcc attgatcaag cagttgccgc
    19621 aaggttcaac gatgatcgcc accgacgtgg cctcgccgtt ctttacgccg atcaagctga
    19681 cctttatggt gtcgctgatt ctgtcagcgc cggtgattct ctatcaggtg tgggcattta
    19741 tcgccccagc gctgtataag catgaacgtc gcctggtggt gccgctgctg gtttccagct
    19801 ctctgctgtt ttatatcggc atggcgttcg cctactttgt ggtctttccg ctggcatttg
    19861 gcttccttgc caataccgcg ccggaagggg tgcaggtatc caccgacatc gccagctatt
    19921 taagcttcgt tatggcgctg tttatggcgt ttggtgtctc ctttgaagtg ccggtggcga
    19981 ttgtgctgct gtgctggatg gggattacct cgccagaaga cttacgcaaa aaacgcccgt
    20041 atgtgctggt tggtgcattc gttgtcggga tgttgctgac gccgccggat gtcttctcgc
    20101 aaacgctgtt ggcgatcccg atgtactgtc tgtttgaaat cggtgtcttc ttctcacgct
    20161 tttacgttgg taaagggcga aatcgggaag aggaaaacga cgctgaagca gaaagcgaaa
    20221 aaactgaaga ataaattcaa ccgcccgtca gggcggttgt catatggagt acaggatgtt
    20281 tgatatcggc gttaatttga ccagttcgca atttgcgaaa gaccgtgatg atgttgtagc
    20341 gcgcgctttt gacgcgggag ttaatgggct actcatcacc ggcactaacc tgcgtgaaag
    20401 ccagcaggcg caaaagctgg cgcgtcagta ttcgtcctgt tggtcaacgg cgggcgtaca
    20461 tcctcacgac agcagccagt ggcaagctgc gactgaagaa gcgattattg agctggccgc
    20521 gcagccagaa gtggtggcga ttggtgaatg tggtctcgac tttaaccgca acttttcgac
    20581 gccggaagag caggaacgcg cttttgttgc ccagctacgc attgccgcag atttaaacat
    20641 gccggtattt atgcactgtc gcgatgccca cgagcggttt atgacattgc tggagccgtg
    20701 gctggataaa ctgcctggtg cggttcttca ttgctttacc ggcacacgcg aagagatgca
    20761 ggcgtgcgtg gcgcatggaa tttatatcgg cattaccggt tgggtttgcg atgaacgacg
    20821 cggactggag ctgcgggaac ttttgccgtt gattccggcg gaaaaattac tgatcgaaac
    20881 tgatgcgccg tatctgctcc ctcgcgatct cacgccaaag ccatcatccc ggcgcaacga
    20941 gccagcccat ctgccccata ttttgcaacg tattgcgcac tggcgtggag aagatgccgc
    21001 atggctggct gccaccacgg atgctaatgt caaaacactg tttgggattg cgttttagag
    21061 tttgcggaac tcggtattct tcacactgtg cttaatctct ttattaataa gattaagcaa
    21121 tagcatggag cgagcctcac catcgggttc ggtgaaaatg gcctgaaagc cttcgaacgc
    21181 gccttcggta ataatcacct tatcacccgg ataaggggtt gccggatcga caatgtcttt
    21241 cggtttatat accgatagct gatgaatcac cgccgatggg actatcgctg gcgacgcgcc
    21301 aaagcgcacg aagtggctga caccgcgggt cgcgttgata gtcgtggtat gaatcacttc
    21361 tggatcaaat tcgacaaaca ggtagttggg aaacaatggc tcactgactg cagtacgttt
    21421 gccacgcacg attttttcca gggtgatcat cggtgccagg caattcacag cctgtctttc
    21481 gaggtgttcc tgggcacgtt gaagttgccc gcgcttgcag tacagtaaat accaggattg
    21541 cataatgact cttatccgtt taatcggggc gcaagaatag caaaagcttt acgctaagtt
    21601 aattatattc cccggtttgc gttataccgt ccgagttcac gctaatttaa caaatttaca
    21661 gcatcgcaaa gatgaacgcc gtataatggg cgcagattaa gaggctacaa tggacgccat
    21721 gaaatataac gatttacgcg acttcttgac gctgcttgaa cagcagggtg agctaaaacg
    21781 tatcacgctc ccggtggatc cgcatctgga aatcactgaa attgctgacc gcactttgcg
    21841 tgccggtggg cctgcgctgt tattcgaaaa ccctaaaggc tactcaatgc cggtgctgtg
    21901 caatctgttc ggtacgccaa agcgcgtggc gatgggcatg gggcaggaag atgtttcggc
    21961 gctgcgtgaa gttggtaaat tattggcgtt tctgaaagag ccggagccgc caaaaggttt
    22021 ccgcgacctg tttgataaac tgccgcagtt taagcaagta ttgaacatgc cgacaaagcg
    22081 gctgcgcggt gcgccctgcc aacaaaaaat cgtctctggc gatgacgtcg atctcaatcg
    22141 cattcccatt atgacctgct ggccggaaga tgccgcgccg ctgattacct gggggctgac
    22201 agtgacgcgc ggcccgcata aagagcggca gaatctgggc atttatcgcc agcagctgat
    22261 tggtaaaaac aaactgatta tgcgctggct gtcgcatcgc ggcggcgcgc tggattatca
    22321 ggagtggtgt gcggcgcatc cgggcgaacg tttcccggtt tctgtggcgc tgggtgccga
    22381 tcccgccacg attctcggtg cagtcacccc cgttccggat acgctttcag agtatgcgtt
    22441 tgccggattg ctacgcggca ccaaaaccga agtagtaaag tgtatctcca atgaccttga
    22501 agtgcccgcc agtgcggaga ttgtgctgga agggtatatc gaacaaggcg aaactgcgcc
    22561 ggaagggccg tatggcgacc acaccggtta ctataatgaa gtcgatagtt tcccggtatt
    22621 taccgtgacg catattaccc agcgtgaaga tgcgatttac cattccacct ataccgggcg
    22681 tccgccagat gagcccgcgg tgctgggtgt cgcactgaac gaagtgtttg tgccgattct
    22741 gcaaaaacag ttcccggaaa ttgtcgattt ttacctgccg ccggaaggct gctcttatcg
    22801 cctggcggta gtgacaatca aaaaacagta cgccggacac gcgaagcgcg tcatgatggg
    22861 cgtctggtcg ttcttacgcc agtttatgta cactaaattt gtgatcgttt gcgatgatga
    22921 cgttaacgca cgcgactgga acgatgtgat ttgggcgatt accacccgta tggacccggc
    22981 gcgggatact gttctggtag aaaatacgcc tattgattat ctggattttg cctcgcctgt
    23041 ctccgggctg ggttcaaaaa tggggctgga tgccacgaat aaatggccgg gggaaaccca
    23101 gcgtgaatgg ggacgtccca tcaaaaaaga tccagatgtt gtcgcgcata ttgacgccat
    23161 ctgggatgaa ctggctattt ttaacaacgg taaaagcgcc tgatgcgcgt ttgttttgcc
    23221 ctatttatcg atccgacaga gaaagcgcat gacaacctta agctgtaaag tgacctcggt
    23281 agaagctatc acggataccg tatatcgtgt ccgcatcgtg ccagacgcgg ccttttcttt
    23341 tcgcgctggt cagtatttga tggtagtgat ggatgagcgc gacaaacgtc cgttctcaat
    23401 ggcttcgacg ccggatgaaa aagggtttat cgagctgcat attggcgctt ctgaaatcaa
    23461 cctttacgcg aaagcagtca tggaccgcat cctcaaagat catcaaatcg tggtcgatat
    23521 tccccacgga gaagcgtggc tgcgcgatga tgaagagcgt ccgatgattt tgattgcggg
    23581 cggcaccggg ttctcttatg cccgctcgat tttgctgaca gcgttggcgc gtaacccaaa
    23641 ccgtgatatc accatttact ggggcgggcg tgaagagcag catctgtatg atctctgcga
    23701 gcttgaggcg ctttcgttga agcatcctgg tctgcaagtg gtgccggtgg ttgaacaacc
    23761 ggaagcgggc tggcgtgggc gtactggcac cgtgttaacg gcggtattgc aggatcacgg
    23821 tacgctggca gagcatgata tctatattgc cggacgtttt gagatggcga aaattgcccg
    23881 tgacctgttt tgcagtgagc gtaatgcgcg ggaagatcgc ctgtttggcg atgcgtttgc
    23941 atttatctga gatataaaaa aacccgcccc tgacaggcgg gaagaacggc aactaaaact
    24001 ttattcagtg gcatttagat ctatgtcgca tccggcattc ggcacacgat gcctgatgcg
    24061 acgctggcgc gtcttatcag gcctacattg gtgccggatc ggtagaccgg ataaggcgtt
    24121 cacgccgcat ccggcaagtg gttaaacccg ctcaaacacc gtcgcaatac cctgacccag
    24181 accgatacac atcgtcgcca gaccaaactg aacgtctttg tgttccatca gattcagcag
    24241 cgtggtgctg atacgcgcac cggaacaacc cagcggatga cccagcgcga tcgcaccacc
    24301 gttgaggttg atcttctcgt caatctgctc aattagtccc agatctttaa tacatggcag
    24361 gatctgcgcg gcaaaggctt cattcatttc aaacacgccg atatcactga cagaaagccc
    24421 cgcttttttc agcgccagtt tcgaggccgg aaccgggccg taacccataa tcgatgggtc
    24481 acaaccaacg accgccatcg aacgcacacg agcgcgcggc ttaagaccta attcatgggc
    24541 gcggctttca ctcatcacca gcatggcagc tgcgccatcg gaaagtgcag aagatgtgcc
    24601 cgccgttacc gtaccgttta ctggatcaaa cgccggacgc agcgtggcga gggcttccac
    24661 ggtggtttcc gggcgaatca cttcgtcgta attaaactgc ttcaggacgc cgtcggcatc
    24721 gtgaccaccg gtcgggatga tttcattttt aaatgcgccc gactgcgtgg cggcccaggc
    24781 gcgggcgtgt gaccgcgcgg caaaggcatc ctgcatttca cggctgatac cgtgcatacg
    24841 cgccagcatt tctgccgtta agcccatcat gcccgccgct ttggcgacat tgcggctcag
    24901 gccagggtga aaatcgacgc cgtgactcat cggcacatgg cccatatgct ccacgccgcc
    24961 aaccagacat gcctgcgcat cgccggtcat gatcattcgt gctgcgtcat gcagtgcctg
    25021 catggatgaa ccacacaagc gattgacagt aaccgccggg acagagtgcg gcacttctgc
    25081 cagcagcgcc gcgttacggg cgatattaaa accctgctcc agcgtctgct gcacacaacc
    25141 ccagtaaata tcgtcgaggg ccgccgcttc cagcgccggg ttacgcgcca gcaggctacg
    25201 cattaaatga gcggagagat cttccgcacg cacgttacga aaagcaccgc ccttcgaacg
    25261 gcccatcggg gtgcgaattg catcgacaat gacaacctgt tccattgtga ctccttaagc
    25321 cgttttcagg tcgccaaccg gacgggctgg ctcaaccggg ggatagtacg gttcgttatg
    25381 acgcgcttta ttacgcagac cttccggcac ttcatacagc gggccgaggt gctgatattg
    25441 ctgtgccata tcgaggtatt ttgcgctgcc gagggtgtcc agccagcgga acgcgccgcc
    25501 gtggaacgga gggaagccca ggccgtagac cagcgccata tccgcttctg ccggagtggc
    25561 gataatgcct tcttccagac aacgtaccac ttcgttgacc atcgggatca tcatgcgggc
    25621 gataatctct tcttcgctga aatcgcgctt cggctggctc acttctacta gcaggtcgtc
    25681 aacagcggcg tcttcttctt tcttcggctt acctttgctg tcttctttat aacgccagaa
    25741 accgaggccg ttcttctgac caaagcggtt ggcatcaaac agcgcgtcga tggcatcgcg
    25801 ataatctttc tgcatccgct gcgggaagcc tgctgccatc accgcctgag cgtgatgcgc
    25861 ggtgtcaatg cccacaacgt ccagcagata tgccggaccc ataggccagc caaactgttt
    25921 ttccatcact ttgtcgatct tgcggaaatc cgcgccgtcg cgcagcaact ggctgaaacc
    25981 ggcgaaatac gggaacagca cgcggttgac gaagaagccg gggcaatcgt taaccacaat
    26041 cggcgtttta cccatcttgc tcgcccaggc gacgactttc gcaatagttt cgtcggagct
    26101 tttctcgccg cgaataattt ccaccaacgg cattcggtgg accgggttaa agaagtgcat
    26161 cccgcagaag ttttccggac gttccagcgc gttggccagt tcgctgatag gaatggttga
    26221 agtgttagac gccagcacgg tatccgggcg tactttttgt tcggtttctg ccagtacggc
    26281 ttttttcact ttcgggtttt cgacaaccgc ttctaccaca acatccacgc ggtcaaaccc
    26341 ggcgtagtcg agcgttgggt ggattgtaga gatcacgcca gccagtttca gaccatcgat
    26401 cttgccgcgc tcaagctgct tgttcagcag tttcgcggct tcggtcatgc cgagggttaa
    26461 cgacttgtcg ttgatatctt tcatgacaac cggcacgcct ttccacgcag actggtaagc
    26521 gatgccgcca cccataatgc ctgcgcccag cacggcggcc tgtttcggcg tttcaacgtc
    26581 tttggtgagt tttttcgctt tgccttttac atattgatcg ttaaggaaaa taccgaccag
    26641 tgcgcgagct tcgttggtat gcgccagcgg gacaaaactt ttgttttcca ggtttaaggc
    26701 ttcttcacga ccaaaacggg ccgcagcttc aatggttttt actgcggtga tgggggccgg
    26761 ataatgtttc cccgccgttt gtgcgaccat ccctttagcg atggtgaagc tcatggtagc
    26821 ttcaatcttg ctcagtttta gcggttccag cttcggctga cgtttcgctt tccagtcgag
    26881 gtcgccgtta atggcctggc gtaaaatcgc catcgcccct tcaatcagtt tttctgcttt
    26941 gactacgcca tccaccagac cgattttcag cgcctggtcc gcgccgacat ctttaccggc
    27001 ggcaatgatt tccagcgcac tgtcagcgcc cagcatacgt ggcatacgta cagaaccgcc
    27061 aaagcccggc atgatgccca gtttggtttc cggcagaccg atgcgcagat ccggcgtcgc
    27121 cagacggtaa tcggtcgcca gcacgcattc gcagccaccg cccagcgcat agccattgac
    27181 ggcagcaatg gtcggcaccg gcagatcttc caggcgatta aacacgctat tggcaaaatg
    27241 cagccactgg cttaactgtt cttcgggaac gaggaacaga gacaaaaatt cggtgatatc
    27301 ggcaccgacg ataaaggccg ctttgttcga acgcagcagc agccctttta gatctgattg
    27361 ctgttccagc acgccgatgg cctcgccgag gctggcgacg gtcgcagtgt cgagtttatt
    27421 aactgaacct ggggcatcaa ataccagttc ggcaatgcca tcttccagcc agtcaaggta
    27481 cagggtgtcg cctttgtaaa gcatgtcagt ctcctgaatc cgcaaggtga tctggtcgta
    27541 ccagatgagt cgaagtgtgt attttgtgtt aaaaatatgc aaataaaaga ttaaagaaat
    27601 gccgatctga tcacgctcgg cagaaatcac gctctggatg aacgatgtgc taagatgcgg
    27661 agacttaagg tcaaaaaaac agaagggtaa aaaatggaat cactggcctc gctctataaa
    27721 aatcatatag ctaccttaca agaacggact cgcgatgcgc tggcgcgctt caagctggat
    27781 gcgttactta ttcactccgg cgagctgttc aatgtttttc tcgacgatca tccctatccg
    27841 tttaaagtga acccgcaatt caaagcgtgg gtgccggtaa ctcaggtgcc aaactgctgg
    27901 ttgctggtgg atggcgtgaa caagccgaaa ctgtggtttt atctgccggt tgattactgg
    27961 cacaacgtcg aaccgctgcc gacctccttc tggactgaag atgtggaagt gatcgcgctg
    28021 ccgaaagccg atggcattgg tagcctgctg cctgctgcgc gcggcaatat cggttatatc
    28081 ggtccggtgc cggagcgtgc gctgcaactg ggtattgagg ccagcaacat caacccgaaa
    28141 ggggttatcg actacctgca ttactatcgc tccttcaaaa ccgagtacga actggcctgt
    28201 atgcgtgaag cgcagaaaat ggcggtcaac ggtcatcgtg cggcagaaga agcgttccgt
    28261 tctggcatga gcgagttcga tatcaacatt gcctatctga ccgcgaccgg tcatcgtgat
    28321 accgacgtac cttacagcaa cattgtggcg cttaacgaac acgctgcggt gctgcattac
    28381 accaaactgg atcatcaggc gccggaagag atgcgcagct tcctgctgga tgctggggcc
    28441 gaatataacg gctatgccgc tgacctgacc cgtacctggt cggcaaaaag tgacaacgat
    28501 tacgcacagc tggtgaaaga cgtaaatgat gaacaactgg cgctgatcgc gaccatgaaa
    28561 gctggcgtta gctatgtgga ttaccacatc cagttccatc agcgcatcgc caaattgctg
    28621 cgtaaacatc aaatcatcac cgatatgagt gaagaagcga tggtcgaaaa cgatctcacc
    28681 ggaccgttta tgccgcacgg tatcggccat ccgctgggcc tgcaggtgca tgacgtcgcc
    28741 ggttttatgc aggatgatag cggtacgcac ctcgcggcac cggcaaaata tccgtacctg
    28801 cgctgcaccc gtattctcca gccgggcatg gtgttaacca tcgaaccggg tatctacttc
    28861 attgaatcgc tactggcacc gtggcgtgaa gggcagttca gcaagcactt caactggcag
    28921 aaaattgaag cactgaaacc gttcggcggc attcgtatcg aagacaacgt ggtgatccac
    28981 gaaaacaacg tggaaaacat gacccgggat ctgaaactgg cgtgatggaa agctggttaa
    29041 ttcctgcggc accggtcacg gtcgttgaag agatcaaaaa gagccgcttc ataacgatgt
    29101 tggcgcatac cgatggcgtt gaggcggcga aagcgtttgt tgaatcggtg cgggcagaac
    29161 accccgatgc ccggcaccat tgcgtggcgt gggtcgcggg tgcgccggat gattctcaac
    29221 agctgggttt ctctgacgac ggggagccgg cgggaacggc aggtaaaccg atgctcgccc
    29281 agctaatggg cagcggcgtc ggggaaatta ccgctgtggt agtgcgctac tacggcggca
    29341 tattgcttgg caccggtggg ttagtgaaag cgtatggcgg cggcgtgaat caggcgctgc
    29401 gccagctaac gacccaacgc aagacgccat taaccgaata tactttgcaa tgtgaatata
    29461 gtcagttaac cggcattgaa gcgttgctgg ggcagtgtga cggcaaaatt atcaacagtg
    29521 attatcaggc attcgttctg ctgcgggtgg cgcttccggc ggcgaaagtg gctgaatttt
    29581 ccgcaaagct ggcggatttt agccgtggtt cattgcaatt gttagcgatt gaagaataat
    29641 ccccacttcg ttttgcagaa ctaaggaagc ggcagagatg cattttcgcg ccattacccg
    29701 aatcgttgga ctactggtca tcttattttc agggaccatg attatccctg ggctggtagc
    29761 actcatctac cgggatggag cgggccgcgc ttttacccag accttttttg tcgccctcgc
    29821 cattggctct atgctgtggt ggccgaaccg caaagagaag ggcgaactga aatcccgtga
    29881 ggggtttctg atagtggtgc tgttctggac cgtgctgggt agtgtcggtg cgctcccttt
    29941 tatcttctcg gaaagcccga acctcacgat taccgatgcg ttttttgaat ctttctctgg
    30001 cctgaccacc accggggcca ctacgctggt ggggctggat tcgctccctc acgccatcct
    30061 cttttatcgc cagatgctgc aatggtttgg cgggatgggg atcatcgtgt tagcggttgc
    30121 gatactgcct atcctcggcg tgggtgggat gcagctctat cgtgcagaaa tgcccggccc
    30181 gctgaaagat aacaaaatgc gcccgcgaat tgcggaaacg gcgaaaaccc tgtggttgat
    30241 ttatgtcttg ctgaccgtcg cctgtgcgct ggcgttgtgg tttgccggaa tggatgcctt
    30301 tgatgccatc ggccatagct ttgcgactat cgctattggc ggcttctcga cacatgatgc
    30361 cagtatcggt tatttcgata gcccgactat taacactatc attgctatct tcctgctgat
    30421 ctccggctgt aactacggtc tgcacttttc actgttaagt gggcgtagtc tgaaggttta
    30481 ttggcgcgat ccggaatttc gcatgtttat cggcgtacag tttacgctgg tggttatttg
    30541 taccctcgta ctgtggtttc ataatgtcta cagttcggcg ctgatgacaa ttaaccaggc
    30601 gtttttccag gtggtgtcga tggcgacaac cgccgggttt acgactgaca gcattgcccg
    30661 ctggccgctc tttttgccgg tactgctttt atgttcggct tttatcggcg gttgtgccgg
    30721 gtcaacgggc ggtggcctga aagtgatccg catcctgctg ctgtttaagc aggggaaccg
    30781 tgagctgaaa cgactggtgc atccgaacgc cgtctatagc attaagctgg ggaatcgcgc
    30841 actgccggaa cgtatcctcg aagccgtttg gggatttttc tccgcctatg cattggtgtt
    30901 tattgtcagt atgctggcga ttatcgccac gggcgtggat gacttttctg cctttgcctc
    30961 agttgttgcg acattgaata acctggggcc agggcttggc gtggttgctg ataactttac
    31021 cagtatgaac ccggtggcta aatggatcct gattgccaac atgctgtttg gtcgtctcga
    31081 ggtctttaca ttgctggtgc tctttacccc gactttctgg cgtgaatgat ggagtaatac
    31141 gtgaaaacat taattctttt ctcaacaagg gacggacaaa cgcgcgagat tgcctcctac
    31201 ttggcttcgg aactgaaaga actggggatc caggcggatg tcgccaatgt gcaccgcatt
    31261 gaagaaccac agtgggaaaa ctacgaccgt gtggtcattg gtgcttctat tcgctatggt
    31321 cactaccact ctgcgttcca ggaatttgtc aaaaagcatg cgacgcggct gaattcgatg
    31381 ccgagcgcct tttactccgt aaacctggtg gcgcgtaaac cggagaagcg cactccacag
    31441 accaacagct acgcgcggaa gtttctgatg aactcgcaat ggcgtcccga tcgctgcgcg
    31501 gtcattgccg gggcgctgcg ttacccacgt tatcgctggt acgaccgttt tatgatcaag
    31561 ctgattatga agatgtcagg cggtgaaacg gatacgcgca aagaagttgt ctataccgat
    31621 tgggagcagg tggcgaattt cgcccgagaa atcgcccatt taaccgacaa accgacgctg
    31681 aaataagcat aaagaa
//
LOCUS       DAZUCE010000030        31438 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZUCE010000030 DAZUCE010000000
VERSION     DAZUCE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.030"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..442
                     /gene="cmtB"
                     /locus_tag="ACHY03_001215"
     CDS             <1..442
                     /gene="cmtB"
                     /locus_tag="ACHY03_001215"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFS3904474.1"
                     /translation="RLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQA
                     IKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIGL
                     SAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            470..1858
                     /gene="cmtA"
                     /locus_tag="ACHY03_001216"
     CDS             470..1858
                     /gene="cmtA"
                     /locus_tag="ACHY03_001216"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFS3904475.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAKSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1873..3150
                     /gene="yggP"
                     /locus_tag="ACHY03_001217"
     CDS             1873..3150
                     /gene="yggP"
                     /locus_tag="ACHY03_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFS3904476.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLNAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3147..4112
                     /gene="glpX"
                     /locus_tag="ACHY03_001218"
     CDS             3147..4112
                     /gene="glpX"
                     /locus_tag="ACHY03_001218"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFS3904477.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4134..4643
                     /gene="fumE"
                     /locus_tag="ACHY03_001219"
     CDS             4134..4643
                     /gene="fumE"
                     /locus_tag="ACHY03_001219"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFS3904478.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     EAPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4640..5353
                     /locus_tag="ACHY03_001220"
     CDS             4640..5353
                     /locus_tag="ACHY03_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFS3904479.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIKRVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5325..6002)
                     /locus_tag="ACHY03_001221"
     CDS             complement(5325..6002)
                     /locus_tag="ACHY03_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3904480.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     QPDDIHP"
     gene            complement(5996..6673)
                     /locus_tag="ACHY03_001222"
     CDS             complement(5996..6673)
                     /locus_tag="ACHY03_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS3904481.1"
                     /translation="MVTLEQFRYCPTHTTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6661..7368)
                     /locus_tag="ACHY03_001223"
     CDS             complement(6661..7368)
                     /locus_tag="ACHY03_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFS3904482.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLH"
     gene            complement(7369..7947)
                     /locus_tag="ACHY03_001224"
     CDS             complement(7369..7947)
                     /locus_tag="ACHY03_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFS3904483.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7970..8401)
                     /locus_tag="ACHY03_001225"
     CDS             complement(7970..8401)
                     /locus_tag="ACHY03_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFS3904484.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8773..9792
                     /gene="epd"
                     /locus_tag="ACHY03_001226"
     CDS             8773..9792
                     /gene="epd"
                     /locus_tag="ACHY03_001226"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFS3904485.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRSEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9842..11005
                     /gene="pgk"
                     /locus_tag="ACHY03_001227"
     CDS             9842..11005
                     /gene="pgk"
                     /locus_tag="ACHY03_001227"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFS3904486.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11220..12299
                     /gene="fbaA"
                     /locus_tag="ACHY03_001228"
     CDS             11220..12299
                     /gene="fbaA"
                     /locus_tag="ACHY03_001228"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFS3904487.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12363..12461)
                     /locus_tag="ACHY03_001229"
                     /pseudo
     CDS             complement(12363..12461)
                     /locus_tag="ACHY03_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12658..13518
                     /gene="mscS"
                     /locus_tag="ACHY03_001230"
     CDS             12658..13518
                     /gene="mscS"
                     /locus_tag="ACHY03_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFS3904488.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13657..14292
                     /gene="argO"
                     /locus_tag="ACHY03_001231"
     CDS             13657..14292
                     /gene="argO"
                     /locus_tag="ACHY03_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFS3904489.1"
                     /translation="MISYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14385..15125
                     /gene="yggE"
                     /locus_tag="ACHY03_001232"
     CDS             14385..15125
                     /gene="yggE"
                     /locus_tag="ACHY03_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFS3904490.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15293..16189
                     /gene="ygfI"
                     /locus_tag="ACHY03_001233"
     CDS             15293..16189
                     /gene="ygfI"
                     /locus_tag="ACHY03_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3904491.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16186..17664)
                     /gene="scpC"
                     /locus_tag="ACHY03_001234"
     CDS             complement(16186..17664)
                     /gene="scpC"
                     /locus_tag="ACHY03_001234"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFS3904492.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17688..18473)
                     /gene="scpB"
                     /locus_tag="ACHY03_001235"
     CDS             complement(17688..18473)
                     /gene="scpB"
                     /locus_tag="ACHY03_001235"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFS3904493.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMSAFLEKRKPNFVGH"
     gene            complement(18484..19479)
                     /gene="meaB"
                     /locus_tag="ACHY03_001236"
     CDS             complement(18484..19479)
                     /gene="meaB"
                     /locus_tag="ACHY03_001236"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFS3904494.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIRDGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAETAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19472..21616)
                     /gene="scpA"
                     /locus_tag="ACHY03_001237"
     CDS             complement(19472..21616)
                     /gene="scpA"
                     /locus_tag="ACHY03_001237"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFS3904495.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARTIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHTENLLAAAVNAARVRVTLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     QEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21820..22713)
                     /gene="argP"
                     /locus_tag="ACHY03_001238"
     CDS             complement(21820..22713)
                     /gene="argP"
                     /locus_tag="ACHY03_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFS3904496.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22855..23085
                     /locus_tag="ACHY03_001239"
     CDS             22855..23085
                     /locus_tag="ACHY03_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3904497.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            23141..23800
                     /gene="rpiA"
                     /locus_tag="ACHY03_001240"
     CDS             23141..23800
                     /gene="rpiA"
                     /locus_tag="ACHY03_001240"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFS3904498.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24056..25288
                     /gene="serA"
                     /locus_tag="ACHY03_001241"
     CDS             24056..25288
                     /gene="serA"
                     /locus_tag="ACHY03_001241"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFS3904499.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRVAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25509..25568
                     /gene="ibsC"
                     /locus_tag="ACHY03_001242"
     CDS             25509..25568
                     /gene="ibsC"
                     /locus_tag="ACHY03_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFS3904500.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(25669..26271)
                     /gene="fau"
                     /locus_tag="ACHY03_001243"
     CDS             complement(25669..26271)
                     /gene="fau"
                     /locus_tag="ACHY03_001243"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFS3904501.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26292..26475)
                     /gene="ssrS"
                     /locus_tag="ACHY03_001244"
     ncRNA           complement(26292..26475)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHY03_001244"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26517..26846)
                     /gene="zapA"
                     /locus_tag="ACHY03_001245"
     CDS             complement(26517..26846)
                     /gene="zapA"
                     /locus_tag="ACHY03_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFS3904502.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27014..27592
                     /gene="ygfB"
                     /locus_tag="ACHY03_001246"
     CDS             27014..27592
                     /gene="ygfB"
                     /locus_tag="ACHY03_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFS3904503.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            27618..28943
                     /gene="pepP"
                     /locus_tag="ACHY03_001247"
     CDS             27618..28943
                     /gene="pepP"
                     /locus_tag="ACHY03_001247"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFS3904504.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     QGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            28940..30118
                     /gene="ubiH"
                     /locus_tag="ACHY03_001248"
                     /gene_synonym="visB"
     CDS             28940..30118
                     /gene="ubiH"
                     /locus_tag="ACHY03_001248"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFS3904505.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQRHVEVTLECGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30141..31343
                     /gene="ubiI"
                     /locus_tag="ACHY03_001249"
                     /gene_synonym="visC"
     CDS             30141..31343
                     /gene="ubiI"
                     /locus_tag="ACHY03_001249"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFS3904506.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         7494 a         7819 c         8495 g         7630 t
ORIGIN      
        1 gcggcttagt gattattttc cagaatcatc aatctcagtt atacattcag caaaagattg
       61 gcaggaagct atcgatttct cgatggtatc attgctggat aaaaactata tcagcgagaa
      121 ttacattcag gcaattaaag attccaccat taacaatggc ccttattata ttctcgcacc
      181 aggcgtggca atgcctcatg cgcgaccgga atgtggggcg cttaaaaccg ggatgtcttt
      241 gacattactt gaacaaggtg tttattttcc ggggaatgac gaaccaatta aattactcat
      301 cggactctct gctgccgatg ccgattcgca cattggcgct attcaggcgt taagtgaatt
      361 actgtgcgaa gaagaaatac tcgaacaact cttaacagca tcatcagaaa aacaattagc
      421 ggacattatc agccgcggat aattactctc tcctctacag gataatatta tggaaaacaa
      481 gtctgctcgt gcaaaggtcc aggcttttgg gggctttttg actgcaatgg tcatccccaa
      541 tattggtgct tttattgcct ggggttttat tactgcgtta tttattccca ccggttggct
      601 gcctaacgaa catttcgcca aaattgtcgg cccgatgatt acctatttat tgcccgtgat
      661 gattggttct accggtggtc atctggtcgg cggtaaacgc ggcgcggtca tgggcggaat
      721 aggtactatt ggtgtgatcg tcggcgcaga gatcccgatg ttccttggct caatgattat
      781 ggggccgctc ggtggtctgg tcataaaata tgttgataag gcactggaaa aacgcatacc
      841 tgccggtttt gagatggtta tcaataactt ctcattaggt atcgcgggga tgctcctttg
      901 cctgctgggt tttgaagtga tcggcccggc ggtgttaatt gccaatactt tcgtcaaaga
      961 gtgtattgag gcgctggtac atgcgggtta tctgcctctg ctgtcagtca tcaatgaacc
     1021 ggcgaaagtg cttttcctta ataatgcgat cgatcagggc gtctattatc cgctgggaat
     1081 gcaacaggct tcggttaacg gtaaatccat cttctttatg gtggcctcta acccaggtcc
     1141 gggcctgggg ctgctgctgg cgtttacctt gtttggtaaa gggatgagta aacgttctgc
     1201 gcccggggcg atgattattc acttcctcgg tgggatccac gaactgtatt tcccatatgt
     1261 gctgatgaag ccgctgacta ttattgccat gattgcgggc ggtatgtctg gcacctggat
     1321 gtttaactta ctggacggtg gtctggtggc tggcccaagt ccggggtcta tctttgctta
     1381 cctggcgctg acgccgaaag gttcgttcct ggcgacaatt gccggtgtta cggtaggtac
     1441 cctggtgtcc tttgctatca cttcgctgat actgaagatg gaaaaaacgg tggaaacgga
     1501 gagcgaagat gagtttgcta agtcagccaa tgcggttaag gcgatgaaac aagagggtgc
     1561 attctcgtta agcagggtta agcgtatcgc ctttgtttgc gatgcgggga tgggctccag
     1621 tgcgatgggc gcgaccacct tccgtaaacg cctggaaaaa gcggggctgg caattgaagt
     1681 aaaacattac gccatagaaa acgtgcctgc ggatgcggat atcgtcgtta ctcatgccag
     1741 tctggaaggg cgcgtgaaac gtgtgacgga taaaccactg atattgatta ataactatat
     1801 tggcgatcca aaactcgaca ctttatttaa tcaattaacc gccgaacata aacactgatt
     1861 acagaggtaa aaatgaaaac caaagttgct gctatttatg gcaagcggga tgtccgtctg
     1921 cgcgaatttg aactgccaga aattaccgat aatgaattac tggtgagtgt gatttctgac
     1981 agcgtctgtt tatccacctg gaaagcggcg ttactcggta gtgaacataa acgcgtaccc
     2041 gacgatttag aaaatcatcc ggtcattacc gggcatgaat gtgccggggt tattgtcgaa
     2101 gtgggtaaaa atctcactgg caaatataaa aaaggccagc gttttgtatt gcaaccggcg
     2161 atggggttac caagcggata ttcagcgggc tacagctacg aatattttgg cggcaatgcc
     2221 acttatatga ttattcccga aatcgccatt aatttgggct gcgtattacc gtatcacggc
     2281 tcttattttg ctgcggcgtc gctggcagag cctatgtgct gcattattgg tgcttatcat
     2341 gccaattatc acaccacgca atatgtttat gagcatcgca tgggcgtcaa acctggcggc
     2401 aatattgcac tgctggcgtg tgcaggtccg atgggcattg gcgctatcga ttacgccatt
     2461 aacggcggca tacaaccgtc gcgggtggtg gtggtcgata tcgacgacaa acgtctggcg
     2521 caggtacaga agctgctgcc ggtggaactg gcggccagta aaggcattga gctggtgtat
     2581 gtgaatacca aagggatgag cgatcctgta cagatgctgc gggcgctgac aggagatgcc
     2641 gggttcgatg acatttttgt ttatgcggcg gtgcctgctg tcgttgagat ggctgatgaa
     2701 ttactggcgg aagatggctg tctgaacttc tttgccgggc cgacggataa aaacttcaaa
     2761 gtgccgttta atttctacaa cgttcattac aacagcacgc acgtcgtcgg tacatccggc
     2821 ggttcaacgg acgacatgaa agaggcgatt gcccttagcg ccactgggca gttacagccg
     2881 tcgtttatgg tgacccatat cggtggcctg aatgcggtgc cagaaaccgt gctcaatctg
     2941 ccggatatcc ctggcggtaa aaaactcatt tataacggcg tgaccatgcc gctcactgcc
     3001 attgccgatt ttgccgaaaa aggcaaaacc gacccgctgt ttaaagagtt ggcgcggctg
     3061 gttgaggaaa cgcacggcat ctggaatgaa caggccgaga aatatctgct ggcacaattt
     3121 ggcgttgata tcggggaggc cgcgcaatga tgtccctggc gtggccatta tttcgcgtta
     3181 cggaacaggc agcgttggct gcctggccgc aaaccggatg tggcgacaaa aataaaattg
     3241 atggcctggc ggtcaccgcg atgcgccagg cattaaacga cgtcgctttt cgtggacgag
     3301 tagttatcgg cgaaggagaa attgaccatg cgccgatgct gtggattggc gaagaggtgg
     3361 gcaaaggcga tgggccagaa gtcgatatcg cggttgatcc cattgaaggc acgcggatgg
     3421 tggcgatggg gcagagcaat gcgctggcgg tcatggcttt cgcccctcga gatagcctgt
     3481 tacatgcgcc cgatatgtat atgaaaaagc tggttgttaa tcgactggct gccggggcga
     3541 ttgatctgtc tttgcctctg gcggacaact tgcgtaacgt ggcgcgggcg ttaggtaaac
     3601 cgctggacaa gctgcgcatg gttacgctgg ataaaccgcg tctgagtgcc gcgattgaag
     3661 aagcaacaca gctgggcgtg aaggtttttg ccctgccgga tggcgatgtc gccgccagcg
     3721 tgctgacctg ctggcaggat aatccttacg atgtgatgta caccatcggc ggtgcgccgg
     3781 aaggcgtgat ttctgcctgt gccgttaaag cattaggcgg ggatatgcag gccgagctga
     3841 ttgacttttg tcaggctaaa ggggattaca cggaaaatcg gcagattgca gagcaggagc
     3901 gcaagcgttg caaggcaatg ggcgttgacg ttaaccgtgt gtactcgctc gatgaactgg
     3961 tgagggggaa cgatatcctc tttagcgcta cgggcgtgac gggcggtgag ctggtgaacg
     4021 gtatccaaca gacggcgaat ggggtgcgga cgcagacatt actgatcggc ggcgcggacc
     4081 aaacgtgtaa tataatagac tccctgcatt gatggtgatc tatcggaaga ccaatggcga
     4141 cgctgacaga agatgatgtg cttgagcaac tggatgcaca ggacaattta ttttcattta
     4201 tgaaaactgc gcattctatt ttgctccagg ggatacgcca gtttctgccg tcgctgtttg
     4261 tcgataacga tgaagagatc gtggaatatg cagtgaagcc gttactcgcc caaagcggcc
     4321 cgcttgacga tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa atggataaat
     4381 ggctgtacgc cgatatcact catttttccc agttctggca ttacctgaac gaacaggatg
     4441 aagcgcccgg atttgccgat gacatgacct gggattttat cagcaacgtc aatagcataa
     4501 cccgcaatgc gatgctctat gacgcgttaa aagcgatgaa gttcgctgat ttcgccgtct
     4561 ggtcagaggc gcgttttagc gggatggtca aaacggcgct gacgctggca gtaacgacca
     4621 ccttaaagga attaacgccg tgaaaattga attaacggtg aatgggctga acgtgcaagc
     4681 gcagtaccat gatgaagaga taaaacgcgt gcataaaccg ttgctgcgta tgctggcagc
     4741 cttacagacg gtaaatccgc agcggcgtac tgtggttttt ctttgcgctc cgccggggac
     4801 ggggaaatct accctaacca ccttctggga atatctcgcg caacaagacc cggaactgcc
     4861 cgctattcag acgctgccga tggatggttt tcaccattac aatagctggc tggatgcgca
     4921 tcaattgcgc cccttcaaag gcgcaccaga gacatttgac gtagcgaaac tggcggaaaa
     4981 tctgcgccag gtcgtggaag gggattgtac gtggccgcag tacgatcgac aaaagcatga
     5041 tcctgttgaa gatgcgttgc acgttaccgc accactcgtc atcgtcgaag gaaactggtt
     5101 gttgctggac gacgagaagt ggtgccagct tgcgcaattt tgtgatttct cgatctttat
     5161 caatgcgcca gctactgcgt tgcgggaacg actggtgggg cgcaagctgg cgggagggtt
     5221 atcgctggca gacgcagaag ctttttatga ccgtaccgac gggccgaatg ttcgacgggt
     5281 gctggaagag agtctgcctg caaatttgac cttaatgatg accgctacgg gtgaatatcg
     5341 tctggttgat taacgttcct gattagacca tcttccagtc gcaccacacg agagaaatga
     5401 cgccgggtaa atgcggcgtc atgactgatt gctacgacgc tcgtgcctcg ctggcggcac
     5461 ttctccagcc agctttcaaa cacgctcaac caattttcgt caaaatcccg actcggctcg
     5521 tccagcagca atagtggcgg agagagtgct tccaggcagg cgacggcgac catccgtcgc
     5581 tgcgcactat gcagatccaa cgggtgcgcg ctggcagtgt ctgctaactg acaacattgc
     5641 agggcagcat gggtacgttg agtgatttca tccgccgggc atttttgcag tttcaaacca
     5701 aatgcgatct cgtcagcgac ggtgctgtga aaaagttggt tttccgcttc ctgaaacagt
     5761 acgccaatct ttgccgcgcg ctggcggttt ttcaggtttt tcatcgcctg ttgttgcagc
     5821 ataaccgtac cggctgtggg cgtaagaaga cctgccatta ctcgtaacag cgtcgatttc
     5881 cctgcgccat tgtcaccggt cagcgccaac cattcacctt gtttgagttg cagagaaatg
     5941 tcgcaaagac aatccgttgc cgcgccaggc cagcgataag atatctgatt tagcgttaac
     6001 atagtggctg gagcgctccg ttttgcaatt gccacgcttg ctggcagtag ttcaggaaag
     6061 gtgtgcgatg acgttcaaaa agaataatca gcgaaccacg ttcaaacgcc cagtgttgta
     6121 agcgctgcaa cagcatttcg ctggcctgcg gcgtcaggcg gctaaaggct tcatcaagaa
     6181 ttaatagctt tggctgcatc gcgatggcgc aggcgatgac cacgcgctgt gtctctccac
     6241 cggagagtgt tgccgggtga cgatggcgca acggctggca ctcggtcaga gtcagggcgg
     6301 catcaatccg cgccataatc tctgcttcag caagacacag attttctggc ccaaaggcaa
     6361 cttcttcttc cacgctaaag gtacagccgg agagctgcaa atagggcgat tgctggacga
     6421 gctggatggt ggcggattgt tcattaagcg ggagatgccc aatgggcgta ccgaggagcg
     6481 tccccgtgcc tgttatctct cctggcagaa agtccggata ccagcccgcc atcagctgtg
     6541 ccagcgtact tttgccactg ccgttgtcac caaagatggc gaccatcccc ggtttgacat
     6601 agtgaaagtc ataacagaac ggaggatgcg ttgtgtgtgt cgggcaataa cggaactgtt
     6661 ctaatgtaac catatccacg ctccgaattc tgtcagcatc agcaagatca tggtgtaacg
     6721 cgctacgcgt tgtagggtgc tatcggctgg tgcccacaac gttgttcgtc ggttgtggat
     6781 ccgaaatgcc cgcatatcca gcgccgcacc acggacagcc agatcgttaa gcgcattgtg
     6841 tgttagcgga ataattaacg cgggcatcgc acgtaaacgc tgataccagc cctcatccag
     6901 cggcacgccg cgggcacgct gcgcttcatg aataatcgct aattgccgtt ttaactgctc
     6961 aacgaccaat aacggcccgg caaacagata agcgacgcct ggcggcaggc gagaagcaaa
     7021 cagagcgcgg ataaaacatt gcacggggac gtactgcatc cacaactgtg aggttgaaac
     7081 aatcgccaga atccgcaacc agagcgtaat agcatgcgtc caacgctccg gcgaacgcgg
     7141 cgtgccgctt agccattctg ttagccagcc accgtgtacc agccacagcc cggctcccag
     7201 cgagaacatc agccagacga catattttgc ccgccgccgc gtggccttta gcgcaataag
     7261 gcagaaaaag gtcgccgcgc tatagactgg cagtattgtc tgtgtgggca ggataagcgt
     7321 ggtgcaggcc gccaatgccc atagtgttaa tgaggtaaat ggatgcattt agcgcacggc
     7381 agccattgcc ggaaaatggc gcgtggtgcg aatcggcagc tggcgcagta aaagccagac
     7441 aatcaccgct gtaagagttt tatcgaccag gttcgcgcct atcactgtaa ttgccaccga
     7501 ctccaccagg ttttgcccca tcgagtgcat ccaggcaacg aacaaatcgg ctccgctacc
     7561 ggtgacaccg ccgaacagcg cagtacgcag cggtacagcg acgaccgtta ccgcaagcgt
     7621 aataatcacg ccgctgacaa cgacttttgg tagggtgcga aaccaacccg ctcgcgccag
     7681 ccaaccggcg accaggccga tgaccatcgc gacgggtgca aatgcggcgg cgattgggtc
     7741 ggtcagcaat ccccagagta aattggtcag taagccggtc agcattccga tcaccggacc
     7801 aagcagtacc gcgctaataa gtgtgccgat ggagtcgaga aatataggta atttgaccat
     7861 gctggccagt tgcccgccga tcatattgat ggcgatagat ataacgatca acaccagtgc
     7921 ctgactggaa aagtggctac gcgccataag aaaacctctc tgaaagtagt tatttaacgg
     7981 gggaaatatg caactcgtcg taaccagaaa gcgcgcaagg ttggcggcta aacgccagtt
     8041 cctcaaggct accgatcacc agttcaagcg tggtccgcac ggtacagccg ggcatcatat
     8101 gaccgccaag catcgtgccg tgtggatcgg aaacgcagag atgaagatgc tcaccactct
     8161 gttccaacgt gccgtttagt gagatcactt cgaatgtacc gttcaggagc gtggtgcctt
     8221 cttgcccggc atagcgcaag gcgatgtcag tcaggctacc ggtacagcct gcaatccagg
     8281 cggcatgaag ttgctgctgc tgtgcaaaag cgtgtagctg ggaaaaaact tcctgcccgg
     8341 ggaggaggcg cagtgcgtaa aagcgtgcgg aggaagaata tgggtagggc aatgtcatca
     8401 ttggagtact ccgaaactcg gacgtattgc ggataatatc gacgctgcat cagtcatatt
     8461 ctgatataag tcagggtttg tgacggcaca acgaaaagat tttgctacgc ttattcgtga
     8521 ttctgcaaag tggaataaat tccacagggg cggggctgaa aacgtgacgt aagtcagata
     8581 attgttttct ttgactggac aatcattcct tttattccac gtttcgctta tcctagctga
     8641 agcgtttcag tcgattaaat gttcgacaat taaccaatca gtcgcagttt gcgacaggta
     8701 aggtttcccc ggacgatttg ctggattact ctgtctggca aatttgctca gggaaaacct
     8761 tgcaggagat ctatgaccgt acgcgtagcg ataaatggct tcggtcgcat cgggcgtaat
     8821 gtggttcgtg ctttgtatga atccggacgc cgggcggaaa ttaccgtggt ggcaatcaac
     8881 gaactggcgg atgctgcggg catggcgcat ttgttgaaat acgacaccag ccatggccgt
     8941 tttgcatggg aagtacgaca ggaacgcgat caactttttg ttggtgatga cgccatccgc
     9001 gtattgcatg aacgttcact gcaatcgctc ccctggcgtg aacttggcgt tgatgtagtc
     9061 ctcgactgca ccggcgtata tggctcccgc gagcatggcg aagcgcacat tgccgccggg
     9121 gctaaaaaag tgctcttttc acatcctggc agtaacgatc tcgacgcgac cgttgtttac
     9181 ggcgtcaatc aggatcaact tcgttcggaa caccgcatcg tttctaacgc ttcctgtacc
     9241 acgaattgca taattcccgt catcaaattg ttagatgatg cgtacggtat tgagtccggc
     9301 actgtgacca caattcactc cgccatgcac gatcaacagg ttattgatgc ataccatcct
     9361 gacctgcgtc gcacccgggc agccagccag tcgatcattc cggtcgatac taaactggcc
     9421 gccggtatca cacgattttt tccgcaattt aacgatcgct ttgaagcgat tgcggtacgt
     9481 gtgccaacca taaatgtgac ggcaatcgat ttaagcgtga cggtgaagaa acctgtaaaa
     9541 gccaatgaag tcaacctgtt gctgcaaaaa gcagcacaag gtgcatttca tggtatagtt
     9601 gactatacgg aattgccgtt ggtctctgta gattttaacc acgatccgca cagtgccatt
     9661 gtcgatggca cccaaacccg ggtcagtggc gcacacctga tcaaaacgtt ggtctggtgc
     9721 gataacgaat ggggctttgc taaccgaatg ctcgacacga cgttagctat ggctactgtt
     9781 gctttcaggt aagacgcaag cagcgtctgc aaaactttta gaatcaacga gaggattcac
     9841 catgtctgta attaagatga ccgatctgga tcttgctggg aaacgtgtat ttatccgtgc
     9901 ggatctgaac gtaccagtaa aagacgggaa agtaaccagc gacgcgcgta tccgtgcttc
     9961 tctgccgacc atcgaactgg ccctgaaaca aggcgcaaaa gtgatggtaa cttcccacct
    10021 gggtcgtcct accgaaggcg agtacaacga agaattctct cttctgccgg ttgttaacta
    10081 cctgaaagac aaactgtcta acccggttcg tctggttaaa gattacctcg acggcgttga
    10141 cgttgctgaa ggtgaactgg ttgttctgga aaacgttcgc ttcaacaaag gcgagaagaa
    10201 agacgacgaa accctgtcca aaaaatacgc tgcactgtgt gacgtgttcg taatggacgc
    10261 attcggtact gctcaccgcg cgcaggcttc tactcacggt atcggtaaat tcgctgacgt
    10321 tgcgtgcgca ggcccgctgc tggcagctga actggacgcg ctgggtaaag cactgaaaga
    10381 acctgctcgc ccgatggtgg ctatcgttgg tggttctaaa gtatctacca aactgaccgt
    10441 tctggactcc ctgtctaaaa tcgctgacca gctgattgtt ggtggtggta tcgctaacac
    10501 ctttatcgcg gcacaaggcc acgatgtggg taaatccctg tacgaagctg acctggttga
    10561 cgaagcaaaa cgtctgctga ccacctgcaa catcccggtt ccgtctgatg ttcgcgtagc
    10621 aaccgagttc tctgaaaccg ctccggctac cctgaaatct gttaacgacg tgaaagctga
    10681 cgagcagatc ctggatatcg gtgatgcttc cgctcaggaa ctggctgaaa tcctgaagaa
    10741 tgcgaaaacc attctgtgga acggtccggt tggcgtgttc gaattcccga acttccgcaa
    10801 aggtactgaa atcgtggcta acgctatcgc agacagcgaa gcgttctcca tcgctggcgg
    10861 cggcgacact ctggcagcaa tcgacctgtt cggcattgct gacaaaatct cctacatctc
    10921 cactggcggc ggcgcattcc tcgaattcgt ggaaggtaaa gtactgcctg cagtagcgat
    10981 gctcgaagag cgcgctaaga agtaaaaaat cacagggcag ggaaacctgc ccttgtttca
    11041 gcgcgctttt agagcacgca ttttttcata ctctaaataa ttcgagttgc aggaaggcga
    11101 caagagagtg aatctccagg agcttacata agtaagtgac aggggtgagc gaacgcagac
    11161 gcagcacatg caacttgaag tatgacgagt ataaggcccg acgatacagg acaagagaca
    11221 tgtctaagat ttttgatttc gtaaaacctg gcgtaatcac tggtgatgac gtacagaaag
    11281 ttttccaggt agcaaaagaa aacaacttcg cactgccagc agtaaactgc gtcggtactg
    11341 actccatcaa cgccgtactg gaaaccgctg ctaaagttaa agcgccggtt atcgttcagt
    11401 tctccaacgg tggtgcttcc tttatcgctg gtaaaggcgt gaaatctgac gttccgcagg
    11461 gtgctgctat cctgggcgcg atctctggtg cgcatcacgt tcaccagatg gctgaacatt
    11521 atggtgttcc ggttatcctg cacaccgacc actgcgcgaa gaaactgctg ccgtggatcg
    11581 acggtctgct ggacgcgggt gaaaaacact tcgctgctac cggtaagccg ctgttctctt
    11641 ctcacatgat cgacctgtct gaagaatctc tgcaagagaa catcgaaatc tgctctaaat
    11701 acctggagcg tatgtccaaa atcggcatga ctctggaaat cgaactgggt tgcaccggtg
    11761 gtgaagaaga cggcgtggac aacagccaca tggacgcttc tgcactgtac acccagccgg
    11821 aagacgttga ttacgcatac accgagctga gcaaaatcag cccgcgtttc accatcgcag
    11881 cgtccttcgg taacgtacac ggtgtttaca agccgggtaa cgttgttctg actccgacca
    11941 tcctgcgtga ttctcaggaa tatgtttcta agaaacacaa cctgccgcac aacagcctga
    12001 acttcgtatt ccacggtggt tccggttcta ctgctcagga aatcaaagac tccgtaagct
    12061 acggcgtagt gaaaatgaac atcgataccg atacccaatg ggcaacctgg gaaggcgttc
    12121 tgaactacta caaagcgaac gaagcttatc tgcagggtca gctgggtaac ccgaaaggcg
    12181 aagatcagcc gaacaagaaa tactacgatc cgcgcgtatg gctgcgtgcc ggtcagactt
    12241 cgatgatcgc tcgtctggaa aaagcattcc aggaactgaa cgcgatcgac gttctgtaag
    12301 tcattccttt ctgcttatct caaggcccgc tctgcgggtc ttttttttgc caaaagaaaa
    12361 attgcctgat gcgctacgct tatcaggcct acacgtatcc tgcaatatat tgaattagca
    12421 ggtttttgta ggccggataa ggcgttcacg ccgcatccgg catgacaaag cgcacattga
    12481 tatctgtctg agccccgctc tgcggggctt tttttcgcca aaagcaaggt gatacagatg
    12541 agaaatctgt gatctatttg gcaaaattat gctttattgt ttacccttgt cagactgccc
    12601 gtcataaggc ggcggagtgt atttctccat tttgagtcag ttgaaaagga atattgaatg
    12661 gaagatttga atgttgtcga tagcataaac ggtgcgggaa gctggctggt ggctaaccag
    12721 gcgctgctgc taagttatgc agtaaacatc gtggcggcac tcgcgatcat catcgttggt
    12781 ttgattatcg cgcggatgat ttccaacgcg gtgaatcgcc tgatgatctc ccgtaaaatc
    12841 gatgccactg ttgctgattt tctttctgca ttagtccgtt acggtattat cgcctttacg
    12901 ctaatcgctg cactgggacg cgtgggtgta caaaccgcgt ccgtcattgc tgtactcggt
    12961 gccgcaggct tagctgttgg tctggctttg caggggtcac tttctaacct ggccgctggc
    13021 gtgttacttg tcatgttccg cccgttccgt gccggagaat atgttgacct cggcggcgta
    13081 gccggtactg tgctgagtgt gcagattttc tccaccacca tgcgtactgc agacggtaaa
    13141 attatcgtta ttccgaacgg taaaattatt gccggaaata ttattaactt ctcccgcgag
    13201 ccagttcgcc gtaacgagtt tattattggc gtggcgtatg attccgatat cgatcaggtt
    13261 aagcagatcc tgaccaatat tatccagtct gaagatcgca ttttgaaaga tcgcgaaatg
    13321 actgtgcgcc tgaacgaact tggtgcatcg tcgattaatt tcgtggtccg cgtctggagc
    13381 aacagcggcg atctgcaaaa cgtgtactgg gatgtgctgg agcgtattaa acgtgaattt
    13441 gatgccgccg gtatcagctt cccgtacccg caaatggatg tgaactttaa gcgggtgaaa
    13501 gaagacaaag ctgcgtaatc aacgctgagg ccagataata ctttctggcc tctctcttat
    13561 tagtttttct gattgccaat taatattatc aatttccgct aataacaatc ccgcgatata
    13621 gtctctgcat cagatactta attcggaata ccaaacgtga tttcttatta ctttcaaggt
    13681 cttgcacttg gtgctgctat gatcctaccg ctcggtccac aaaatgcttt tgtgatgaat
    13741 cagggcattc gtcgtcagta ccacattatg attgccttac tttgcgctat cagcgatttg
    13801 gtcctgattt gcgccgggat ttttggtggc agcgcgttat tgatgcagtc gccgtggttg
    13861 ctggcgctgg tcacctgggg cggcgtagcc ttcttgctct ggtatggttt tggcgctttt
    13921 aaaacagcga tgagcagtaa tattgagtta gccagtgccg aagtcctgaa gcaaggaaga
    13981 tggaaaatta tcgccaccat gttggcagtg acctggctga atccgcatgt ttacctggat
    14041 acttttgttg tactgggcag ccttggtggg cagcttgacg tcgaaccaaa acgttggttt
    14101 gcgctcggga caattagcgc ctctttcctg tggttctttg gtctggctat tctcgcagcc
    14161 tggctggcac cgcgcctgcg cacggcaaaa tcacagcgca ttatcaatct ggtagtggga
    14221 tgtgtcatgt ggtttatagc cttgcagctg gcgagagacg gtattgctca tgcacaagcc
    14281 ttgttcagtt aggcgctatc tgatggaaaa ataaaacaga ggcgctaagc ttgcctccag
    14341 aggtcctgaa tttttggcaa gtcaattaac acggagagac taacgtgaag ttcaaagtta
    14401 tcgccctggc ggcattaatg ggtattagcg ggatggcagc gcaggctaac gaattgccgg
    14461 atggaccgca tattgtcacc tccggtacgg caagcgtgga tgcggtgcca gacattgcca
    14521 ctcttgcgat tgaagttaac gtggccgcga aggatgccgc tactgccaag aaacaggcag
    14581 atgagcgcgt cgcacaatac atttccttcc ttgaactcaa tcagatcgcg aaaaaagata
    14641 tcagctcagc gaacttacgc acccagccag attatgatta tcaggatggt aaaagtatcc
    14701 tgaaaggcta ccgcgcagtg agaacggtgg aagtcacgct ccgtcagtta gacaaactga
    14761 attccttgct ggacggcgcg ctgaaggcgg gtcttaacga aattcgttct gtgtcgctgg
    14821 gcgtggcgca gccggatgcc tataaagaca aagcgcgtaa ggcagcgatt gataacgcga
    14881 ttcatcaggc gcaggaactg gcgaacggct ttcatcgtaa actggggccg gtatatagcg
    14941 tgcgctacca tgtttccaac tatcagccca gcccaatggt gcggatgatg aaagccgatg
    15001 ccgcgccggt gtccgcccag gaaacttacg agcaggccgc tattcagttt gatgatcagg
    15061 tcgatgtggt cttccagtta gaacctgtgg atcaacaacc cgctaaaaca cctgcagcac
    15121 aataatactt gtagcccgac gtattcgtcg ggctaatgcc tttacccgat attgcctcct
    15181 ttttctgtaa ttttctgtaa aaccatgacg taatcaccta tctttgctcg cggtttcatc
    15241 aaggataggc tgactgaaac ggttaagcgg acaaggatgc gggggagggg ggatggacat
    15301 ttttatttcg aaaaagatgc gcaatttcat tttattagcg caaaccaata atattgcccg
    15361 ggcggcagaa aaaatccata tgacggcttc gccatttggt aaaagcattg ccgcgctgga
    15421 agaacaaatt ggctatacgc tatttacccg caaagataat aatatcagcc tcaataaagc
    15481 cggacaggaa ctgtatcaaa aactgttccc ggtttatcaa cggctttctg cgattgataa
    15541 tgaaatccat aactcagggc gtcgttcacg agaaattgtg attggcatag ataatactta
    15601 tccgacaatt attttcgatc agttgattag tctcggagat aagtatgaag gcgtgaccgc
    15661 ccagccagtt gagttcagcg aaaacggcgt tatcgataat ctttttgacc gccagttaga
    15721 ttttattatc tccccacagc atgtatctgc acgagttcag gaactggaaa acctgactat
    15781 cagtgaacta ccaccgctgc gattaggctt tctcgtttct cgccgttatg aagagcgtca
    15841 agagcaagag ctgcttcagg agctgccctg gctacaaatg cgctttcaga accgggccaa
    15901 cttcgaagca atgattgatg cgaatatgcg accttgtggg atcaatccaa ccattattta
    15961 tcgtccctac agttttatgg ccaaaatcag cgccgtggag cgcggacatt ttctgacggt
    16021 gatcccgcat tttgcctggc gactggtgaa tccggcaacg ctgaaatatt ttgatgcgcc
    16081 gcacaggccg atgtatatgc aggaatatct ttattcaatc agaaatcatc gttataccgc
    16141 cacgatgctt cagcatattg ctgaagatcg tgacgggaca aaccattaac ccagcatcga
    16201 gccggttgca attaaattac ggtgtaagtc gaagacgtgg ctaagatcgt ggtgaatatg
    16261 tccgccaggc gcattttcca gatagcgatg cagataatcc cgatacatag gatgtgcaca
    16321 attatcaatg atcgtgcggg cgcgttgaag cggggaaaga ccgcgcagat cggcgatccc
    16381 ttgttcggtg atgatcactt tgacgctgtg ttcgctgtga tcaacatggc tgcacattgg
    16441 cacgacggtt gagatcttgc cttctttagc aatcgacggg gccataaaga tcgacagata
    16501 cgcgttgcgt tcaaaatcac cgctgccgcc gatgccgttc atcagatcga ccccggctac
    16561 gtgcgttgag ttggcatgcc cgtaaatatc aaactccagg ccgacgttca gagcgatgac
    16621 gcccagacga cggatgattt ccgggttatt ggaaatctcc tgcggacgca acacaatgcg
    16681 gctggcaaag taatccatat tgtcgtaaat cttgcgcagg gaatcggccg agattgtcag
    16741 gctggaggcg ctggccccgc tgattttgcc ggtttccagt aaatgcacca ccgattcctg
    16801 tagcacttcc gaatacatca taaacggagg aatttccggg ttttccccca gacgcgccat
    16861 taccgcatta ttgatattgc ccacgccact ttgcagcggc agaaattccg gcggaatacg
    16921 cccatgcgcc atttcctgca ataagaacgt gaccacgtta tcggcaatct gctggcacat
    16981 gggattttgc ttatccagca tattaccggc gtcaggcaag ttggtttcca cgacggcgac
    17041 aatctttttc ggatcgattt gcacatagcg ggtaccgaca cgatccattg catggaagat
    17101 cgacacgcta ttacgccgcg gtggcgcgcc aggaatcaca atatctgcca gttctgcaac
    17161 gcgcggatcg tgatagtgat taagttcaat gatcactttc ttcgcccgca gcagccatgt
    17221 cggcgcatta ccgatcccgc tggttaacca gactcgacca tccggtgcca gtgccgatgc
    17281 ttcgatgacg gcaacatcaa tgtcgccgaa gaaaccgtaa ttgaccattt gcgccacttc
    17341 gctcaaatgc aggtcaacga aactcaccgc gccctgattg atctttttac gtaaaccgga
    17401 cgatgtttga tatggcgcac gccaggaaac agcatcggcg tcagaaagta catcgtcagc
    17461 ggcggcgctg attgacgccc ccgtcagcag gcgaatttga tacggctttt tggcctcatg
    17521 ctgttcgtta gctctgcggg caatcgcggt gggtagggct ttcggcgaac ccgctggggt
    17581 aaagccgcta aatgccacca tgtcgttatg ctggataatt tctgccgctt catcggcggt
    17641 catccttgtc cactgagttt ccatttttac tccttcgttc gcagggatta atgaccaacg
    17701 aaattaggtt tacgtttttc gaggaaagcg ctcatccctt cctggtaatc ttcgctgtca
    17761 taaaccgcgc ggcgcatccc ctgaatacgt tcaaattcat cggagttcat ggtgtgcgct
    17821 tcgcccagta cacgcagctc ttctttgata acggcaatgg ctaacggcgc tttctcagag
    17881 atgtggtgcg ccatttgtaa ggtgaaatct tccagttctt ccacctccac aacatggttg
    17941 aggatgccga cagccagcgc gcgttgggcg gtgattggcg aagcggtaaa aatcagctct
    18001 ttgacaatgt ggaagcccgc gtcgcgggtc aggttgtgaa tgccgaccag gttatacggg
    18061 acgccgaggt ttacaggcgt cattgagaag gttgaggtac tggcggcgat gatcagatcg
    18121 gaactcatga tcatttcaaa tgctccaccc caaacactac cttccaccat cgaaatgatc
    18181 ggtttcggga atttttggat catgcgggtg atttgacgca atggatcatc ataggagagc
    18241 ggatcgcgac cgccagacgg cagttcgtga atatcgtgac ctgcggagaa gactttggat
    18301 ccactcggcg cgcgcaaaat gatacagcga atttccggcc ggttgagatc gcttaacgcc
    18361 tgcataagat catcaataaa gactttactt aaggcattaa gttttcggcc atagttaaac
    18421 tcaatgaccg ccactttgtt gatagtggca acgttcacat actgataaga cataaaaatt
    18481 cctttaatca aaatattgcg tctggataaa ttcactgagc tgccgcaggc cggtgcgcgg
    18541 tgagagcgta ttgtttttga ccgctaaaag cgtctggcgg taatagcgat cgaaatcttc
    18601 attcgcgaac aggtgattca gtacttcttc ttcggtctgc ttacgcagcc attccaccga
    18661 ttgttgttgc cgcacttgtt gtaaacgacc actggcagtt agcgcggttt tgaagtcgat
    18721 gatggcgtgc cagatctcat cgattccacg tttttccagt gcgctacaag tcagaacccg
    18781 tggctgccat tcgtcgtatt tacgtcgcag aatatgcagg gcactctcgt acatatgccg
    18841 ggcaatggcg acattggtat ggttatcgcc atcgtctttg ttgataacga tcagatcagc
    18901 cacttccatc agcccttttt taatgccctg cagatcatcg ccgccaccgg caatttgcaa
    18961 cgagataaaa cagtccacca tgcgggcgac ttccgtttcc gactgcccga cgccaaccgt
    19021 ttcgacaatc actacgtcat aacccgccgc ttcgcataac agcattaatt cccgcgctcg
    19081 ctgactggca ccgcccagat gaccggagga tggtaccggg cgaataaacg ccgtctcggc
    19141 acgcgccagg tcattcatgc gggttttatc cccgagaatg ctaccgccag tgaccggact
    19201 gctgggatcg accgcaataa ccgcaacctt taatccatca cgaatcaaca acatgccaaa
    19261 ggcttcaaga aaggtacttt tccccgcgcc gggtgtgccg gtaacgccca gtcgcagggc
    19321 gttaccgcag tacggcataa tggcatcaag cagttgcgta cttagcgcct gatgacgcgg
    19381 gtgacggctt tccaccagcg tcatggcctg ggcgagtgtg gcacgctcac cctgacgtaa
    19441 gcggcgaata ctttctgcca gcgtggcttc attaatcatg atgctggctt atcagattca
    19501 gtacgtcgcg cacactgtcg agcataggtg tacctggacc ataaatcgcc gccacgccgc
    19561 gctcttgcag gaaggcgtaa tcctgcggtg gaatgacgcc accggcgact acgcagatat
    19621 cttcgcgtcc ccattttttc agcgcttcga ccagttccgg gatcagcgtt ttatgaccgg
    19681 cagccagtga ggatgcgccc actacgtgaa cgtcattttc tactgccagg cgggcgatct
    19741 cttgaggtgt agagaacatc gggcttaaat ctacgtcgaa accgagatcg gaataggcgc
    19801 tggcgatcac tttcgcgccg cgatcgtgcc catcctggcc cattttggcg atcagaatgc
    19861 gcgggcgacg accattgtcg gcaaggaact gctccgtttg cgcaacaatg gcatcgaact
    19921 cggaggccga tttctcagat tgatgatagc tttgcgcaat cacgccggta acacactggc
    19981 ttggcaccag ataacggtcg aaagcggctt ccagcgcatc ggaaatttca cccagggtga
    20041 cgcgaacgcg agcggcatta acagcggcag ccagcaggtt ttcggtatgc tgtgcggcgt
    20101 gagtcagggc gttcaacgcg gcggttacgg cggcatcatc acgggtggcg cgaatgcgtt
    20161 ccagcgaagc aatttgctcg ttacgcacca tcacgttgtc gatctcaagt acatcggttt
    20221 cgtcttcgtg atccagtttg tacttgttga caccaacgat gacacgcttg ccctggtcga
    20281 tcagcgactg ttcgcgcgct gaggcctctt cgatcattcg ttttggcaga cctgcttcga
    20341 tcgctttcgc catgccaccg gcttcgtcga tctgttggat aatagttctg gcttgtttga
    20401 cgatttgatc ggtcagcgac tcaatgtaat aggatccggc cagtggatcg acggtgcggc
    20461 agagttctga ttcttcctgg atgatgatct gggtgttgcg ggcaatgcgt gctgagaaat
    20521 cggtaggcaa accaagcgct tcgtcaaagg cgttggtatg cagtgactga gtaccgccca
    20581 gcgtcgcagc cagtgcttca atggtggtgc ggataacgtt gttatacgga tcctgttcag
    20641 tcaggctcca gcctgaggtc tggcagtggg tacgcagcgc cagtgatttc gggtcctgtg
    20701 cgccaaatcc actgaccgct tcgctccata aataacgtgc cgcacgcaac atggcgacgt
    20761 tcataaacag atccatgccg atgccgaaga agaacgacag gcgaggagcg aagtcatcaa
    20821 ttttcagtcc ggcagagatt gctgctttga tgtactcaat cccatcagcg agcgtaaatg
    20881 ctacctgctg cacgcagttg gcacccgctt cacccatgtg gtaaccgctg atactgatgg
    20941 tattaaatcg cggcatgttg ccggaacacc aggcgatgat gtcggcgata atgcgcattg
    21001 acggttttgg cgggtaaata taggtgttgc ggcagaggta ctctttgagg atatcgttct
    21061 gaatggtgcc ggtcagttta tcaggtgtaa caccttgctc ttccgcggcg acgatataaa
    21121 acgccagtac tggtagcact gcgccattca tggtcatcga aaccgacatt ttatccagcg
    21181 ggatctggtc gaacaggact ttcatatctt ccacggtgtc gatagcgacg cccgctttgc
    21241 cgacgtcgcc cgccacgcgc gggttatcgg agtcgtagcc tcggtgggtg gcaaggtcaa
    21301 atgcaacgga aagacctttt tgcccggcgg ccaggttacg gcgataaaaa gcgttggact
    21361 cttttgctgt tgaaaaacca gcatactgac ggatggtcca cggttgggcg gtatacatag
    21421 tggcacgcgg gccacgaacg tagggcggca aaccaggaag ggtacctgtc acctccagat
    21481 tatcgagatc ggcttcggta tacagcggct tgatggcgat cccttccgcg gtttgctgaa
    21541 ccagcgagtc gacagttttc tcccgacggc tcaattcctt gttggcaagc tgttgccact
    21601 cctgcacgtt agacattatt tgctccgtaa aacgcaatca aaatgagggt atttgcgcct
    21661 acggtgagct ttttacgtgg gtttgtcgcc agcaaaaaaa atgcattcag acgtcgtttt
    21721 ttgccgcctg agatttgtct attttgtgat gtgattcaag gaatacaaaa agaagtgagg
    21781 acaacgcctg atatgtgcta tcaggcatta tttgatggat taatcctgac gaaggacttt
    21841 gtgaccataa tcgagtaacg catcagtgac tttacgcatc atgcggcttt caggtgcaaa
    21901 gcggtgccag taaagcattc ggcgttgaaa tagcccaggc gttaagtcaa tcagttcacc
    21961 gctggccagt tctttctcga tttgcaggtg cgggatcata cagcaggtgg tgccctggcg
    22021 agcaagttgc acgaatgctt ctgaagaatt aacgatatgg cagggtacgc tgccaggagg
    22081 cagatcgaag ttttgctgca aaaaggcctg gtgcatatcg tcaagatggt caaacgcgac
    22141 cactggcgct ttcaataatg ccgaacgcgt tacgccgtta gggaaatatt tttcggcaaa
    22201 gggttttgag ctgacgaaca gatagtcgag cgcaccaagt ttatcgacaa gacaactcgg
    22261 cagcgcctga tgttgaatac tcaccgcgcc gaccacttcg ccgcggcgca gacgttcctg
    22321 agtgcgggtt tcatcttcta cctgcaagtt gaggcggata ggcgaatcag ccaacacagg
    22381 agccagtgca ggaagcaacc acgtcgccag actgtcggca ttgaccgcca gtgaaagcag
    22441 cagcggagtc gaaccggttt gttcatcgcc cagccactct tcttccagca actccacctg
    22501 gcgcagcagt gccagcagtt tttgcccttg ttccgtcggg cgcggcggta cggtacgcac
    22561 caacagcggc tgcccgaaca tattttccag ttgcttaatg cgctgtgaga cggctgattg
    22621 tgtaatgcac agcttttgtg ccgcgcgctc aaatcctcgt tcacgtatca ccgcatccag
    22681 tgcctgtaat gttctgtagt ccgggcgttt cattgctgtt gtctgctcct ggtaaatgct
    22741 ttttcctgca ctatgacaca attttgtgtc aggttgcata taccgaagcc gtcggtgggt
    22801 aacatttgta ttcgtggtta aacgcgcagc ttaaaagatc agggatttgc gctaatgcat
    22861 ttcgctcagc gagttcgcgc cctggttgtg ttgaatggcg tggcgttatt gcctcaattt
    22921 gcctgtaaac aggggcttgc gaacggtgaa ttggtgcgcc tgtttgcacc gtggagcggc
    22981 atacccagac cattgtatgc tttatttgcg gggcgaaagg ggatgcctgc cattgcgcga
    23041 tattttatgg atgagttaac cacgcggctt gccaacgggg tctgaatcgc tttttttgta
    23101 tataatgcgt gtgaaatttc ataccacagg cgaaacgatc atgacgcagg atgaattgaa
    23161 aaaagcagta ggatgggcgg cacttcagta tgttcagccc ggcaccattg ttggtgtagg
    23221 tacaggttcc accgccgcac actttattga cgcgctcggt acaatgaaag gccagattga
    23281 aggggccgtt tccagttcag atgcttccac tgaaaaactg aaaagcctcg gcattcacgt
    23341 ttttgatctc aacgaagtcg acagccttgg catctacgtt gatggcgcag atgaaatcaa
    23401 cggccacatg caaatgatca aaggcggcgg cgcggcgctg acccgtgaaa aaatcattgc
    23461 ttcggttgca gaaaaattta tctgtattgc agacgcttcc aagcaggttg atattctggg
    23521 taaattcccg ctgccagtag aagttatccc gatggcacgt agtgcagtgg cgcgccagct
    23581 ggtgaaactg ggcggtcgtc cggaataccg tcagggcgtg gtgaccgata atggcaacgt
    23641 gatcctcgac gtccacggca tggaaatcct tgacccgata gcgatggaaa acgccataaa
    23701 tgcgattcct ggcgtggtga ctgttggctt gtttgctaac cgtggcgcgg acgttgcgct
    23761 gattggcaca cctgacggtg tcaaaaccat tgtgaaatga tctgacgggg gaacctcccc
    23821 cgttaaaaaa attctcttca ttaaatttgg tgacatgtgt cacgttttta cctggctatt
    23881 gtcgattgct ctaaataaat cctctaaacc agcatattca tccaagaatt acctttgcgt
    23941 gatatttcct caacatcgcg acgcaaacgt tcatattgcc gcaatattat tttttgatat
    24001 gttgaaaggc ggacgcaaat ccgcacacaa catttcaaaa gacaggattg ggtaaatggc
    24061 aaaggtatcg ctggagaaag acaagattaa gtttctgctg gtagaaggcg tgcaccaaaa
    24121 ggcgctggaa agccttcgtg tagctggtta caccaacatc gaatttcaca aaggcgcgct
    24181 ggatgatgaa caattaaaag aatccatccg cgatgcccac ttcatcggcc tgcgatcccg
    24241 tacccatctg actgaagacg tgatcaacgc cgcagaaaag ctggtcgcta ttggctgttt
    24301 ctgtatagga acaaaccagg ttgatctgga tgcggcggca aagcgcggga tcccggtatt
    24361 taacgcaccg ttctcaaata cgcgctctgt tgcggagctg gtgattggcg aactgctgct
    24421 gctattgcgc ggcgtgccgg aagccaatgc taaagcgcac cgtggcgtgt ggaacaaact
    24481 ggcggcgggg tcttttgaag cgcgcggcaa aaagctgggt atcatcggct acggtcatat
    24541 tggtacgcaa ttgggcattc tggctgaatc gctgggaatg tatgtttact tttatgatat
    24601 tgaaaacaaa ctgccgctag gcaacgccac tcaggtacag catctttctg acctgctgaa
    24661 tatgagcgat gtggtgagtc tgcatgtacc agagaatccg tccaccaaaa atatgatggg
    24721 cgcgaaagaa atttcactaa tgaagcccgg ctcgctgctg attaatgctt cgcgcggtac
    24781 tgtggtggat attccagcgc tgtgtgatgc gctggcgagc aaacatctgg cgggggcggc
    24841 aatcgacgta ttcccgacgg aaccggcgac caatagcgat ccatttacct ctccgctgtg
    24901 tgaattcgac aacgtccttc tgacgccaca cattggcggt tcgactcagg aagcgcagga
    24961 gaatattggc ctggaagttg cgggtaaatt gatcaagtat tctgacaatg gctcaacgct
    25021 ctctgcggtg aacttcccgg aagtctcgct gccactgcac ggtgggcgtc gtctgatgca
    25081 catccacgaa aaccgtccgg gcgtgctaac tgcgctgaac aaaatcttcg ccgagcaggg
    25141 cgtcaacatc gccgcgcaat atctacaaac ttccgcccag atgggttatg tggttattga
    25201 tattgaagcc gacgaagacg ttgccgaaaa agcgctgcag gcaatgaaag ctattccggg
    25261 taccattcgc gcccgtctgc tgtactaata ccccttctct gaaaatcaac gggcaggtca
    25321 ctgacttgcc cgttttttta tcccttctcc acaccgttgc gcattctact ttgcgagtat
    25381 gctccagaaa ataaatcaaa gtcagatcaa tgcgttgtgt ttatttaatg gggatttacg
    25441 atggcagggc agcatggggc tgtaacggta aagccctcac cgaagcgagg gcttgaagga
    25501 gaagggttat gatgcgactt gtcatcatac tgattgtact gttactcata agtttcccag
    25561 cttattaaca gtcagtctca ggggaggagt aatcctccct tacccttact cactaaatta
    25621 ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg tgtcgccctt accactccca
    25681 gactttcgac ggtgtaacca ccgcaggaag agggatatcc cactcttcaa cgggaagttt
    25741 ttccactaac tgacaatcat gcgcataacc caccggttgc attttatagt gctgccagtt
    25801 ttgtaaggtc cgatcataaa aaccaccgcc cattcccagg cgctggccgt actcatcaaa
    25861 ggcgaccagc ggtgtgatca acacgtctaa tcgggaaagg ggtagcacgt cacgcacatc
    25921 caattttggc tcatggatct tcaacctgtt catcaccagt tcgctttgcg gatggtaatt
    25981 caggaacagc aaattaccgg cactaaaggg atgcaaaact ggaagatata cgcgcttacc
    26041 ggcgcgccag agttgttcta tgagtggctg ggtgtcgagt tcgccatcaa aagagaggaa
    26101 tacagcgacc gtatgtgcca tcaccaccgg gggataagtc atcatccggg tagcggcttg
    26161 ttgacccatt tcctgctgtt gttccggcgt taacgtacga cgacgttgcc gaatcatttt
    26221 gcggatttct tgtcgggata atgtcagtgg gagttctggt agttgcgtca tggctggtgc
    26281 cagataagaa gggaatctcc gagatgccgc cgcaggctgt aacccttgaa cccttggttc
    26341 aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg gaccgagcat gctcaccaac
    26401 cgcggagcgc cacattcttg tggtatgaaa tatcggctca ggggactggc ccgcttgcga
    26461 acatctcaga gaaattttgt cttcacggtt actctaccac agtaaaccga aaagtgtcat
    26521 tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc ttgttctatg
    26581 gtctgctgca gcatccgaat acgctgttcc atacttgccg cgtagtcacg agtctttgct
    26641 ttttcttgcg ctaactcata gctgatattc aatgcggcaa tgaagaccaa ctgttcagta
    26701 tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc gtccgctgcc
    26761 tgattcaacg catccctttg gtcaggcggg cagttcacac gcagtgaacg gccaaaaatt
    26821 tggatatcga cgggttgtgc agacatgcca ccttcctgct gattgactgc gctgccttcg
    26881 tctttagacc catggtctgc gaaggggcga cactatagct accctgatga gaagagacaa
    26941 gcccttttct ggtccaccag ggcccaaagt ggtagcatat catgaatatt cctccctttg
    27001 acgacgaatg cttatgtcta tacagaacga aatgcctggt tacaacgaaa tgaaccagta
    27061 tctgaaccaa caagggacgg gtctgacccc tgctgagatg catggtttaa tcagtgggat
    27121 gatatgtggc ggtaacgatg acagctcatg gctaccgcta cttcacgacc tgacgaacga
    27181 aggcatggct ttcggtcatg agctggcaca ggcactgcgt aaaatgcact ctgccaccag
    27241 cgatgccctg caggatgacg gcttcctttt tcagctttat ctgcctgatg gcgatgatgt
    27301 cagcgttttc gatcgggctg atgcgctggc aggttgggtc aatcacttcc tgcttggtct
    27361 tggcgttacg caaccgaagc tggacaaagt gaccggcgaa accggtgaag ccatcgacga
    27421 tctgcgtaac atcgcgcagt tgggctacga cgaagacgaa gatcaggaag agcttgaaat
    27481 gtcgcttgaa gagatcatcg aatacgtccg tgttgccgcg ctgttatgcc acgacacctt
    27541 tactcatccg caaccgaccg cgccagaagt acaaaaaccg actctacact aaaaacaaaa
    27601 aacgtaagga gagtgttatg agtgagatat cccggcaaga gtttcagcgt cgccgtcagg
    27661 ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca ccagaagtaa
    27721 cacgtagcgc cgacagcgaa tacccctatc gtcagaacag tgacttctgg tacttcaccg
    27781 gctttaacga accggaagcg gtgctggtgc tgattaaaag cgatgacact cataaccaca
    27841 gcgttctgtt taaccgcgtt cgcgacctga cggcggagat ctggtttggc cgtcgtttag
    27901 gtcaggatgc cgcgccagag aaacagggcg ttgaccgcgc actggcattc agcgaaatta
    27961 atcagcaact ttatcaacta cttaatggtc tggatgtggt ttaccacgcc cagggcgaat
    28021 atgcatatgc tgatgaaatc gtgaacagcg cgcttgagaa actgcgtaaa ggttcgcggc
    28081 aaaatctcac cgcgccggca acgatgatcg actggcgtcc cattgttcat gaaatgcgcc
    28141 tgttcaaatc gccggaagag attgccgtac ttcgccgcgc gggtgaaatc accgccctgg
    28201 cgcatacccg ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccac ctggaaggcg
    28261 aaattcacca cgaatttaac cgccacggcg cgcgctatcc ttcctataac accattgttg
    28321 gcagcggtga aaacggctgc attctgcatt acaccgaaaa cgagtgtgaa ctgcgcgacg
    28381 gcgatctggt gttgattgac gcgggttgcg aatacaaagg ttacgctggc gatattaccc
    28441 gcaccttccc ggtcaacggc aaattcactc aggctcagcg tgaaatctac gacattgtgc
    28501 tggagtctct cgaaaccagc ctgcgcctgt atcgtccggg aacttccatt caggaagtca
    28561 ccggtgaagt ggtgcgcatc atggttagcg gcctggtaaa actcggcatc ctgaaaggtg
    28621 atgttgatga actgatcgct cagaacgccc atcgtccttt ctttatgcat ggccttagcc
    28681 actggttagg actggatgtc catgacgtgg gtgtttatgg tcaggatcgc tcgcgcattc
    28741 tggaaccggg catggtactg accgtagagc cagggctgta tattgcgccg gatgcagaag
    28801 tgccagaaca atatcgcggt atcggcattc gtattgaaga cgacattgtg attaccgaaa
    28861 ccggtaacga aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc gaagcgttga
    28921 tggcagctgc gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg cgggcgcgac
    28981 gctggcgctg gctatttccc ggttaagtca cggagcgctg ccggtacatt tgattgaagc
    29041 gaccgcgcca gagtcacatg ctcatccggg ctttgatgga cgagctattg ctctagcggc
    29101 ggggacctgt cagcaactgg cgcgcatcgg cgtctggcaa tctttggctg attgcgcaac
    29161 cgccatcact accgtgcatg tcagcgatcg tggtcatgcc ggattcgtca ccctcgccgc
    29221 agaagattac caactggcgg cgctggggca ggttgtcgaa ttgcacaatg tcgggcaacg
    29281 gctgtttgca ttgctgcgca aagcacctgg cgtaacgctg cattgcccgg atcgcgtggc
    29341 taacgttgcc cgtactcaga gacatgttga agtgacgctg gagtgtggcg agacgctgac
    29401 gggccgcgtg ctggtagccg ctgatggcac ccattcagcg ttagccactg tgtgcggcgt
    29461 tgactggcag caggagcctt acgaacaact ggctgtgatt gccaacgttg cgacttccgt
    29521 tgcgcatgaa gggcgcgctt ttgaacgctt tacgcaacat ggcccgctgg cgatgttgcc
    29581 gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc gcgaagaggt
    29641 gctgtcgtgg agcgacgaga agttttgccg tgaactccag tcggcctttg gctggcgact
    29701 tgggaaaatt acccacgctg gtaaacgcag tgcttatccg ctggcgttaa cccacgccgc
    29761 cagatctatt acccatcgta ccgtgctggt gggcaatgcg gcgcaaaccc tgcaccccat
    29821 cgctgggcaa ggatttaatc tcggtatgcg tgatgtgatg agccttgcgg aaaccctgac
    29881 tcaggcgcag gagcgcggag aagacatggg tgattacggc gtattgtgcc gttatcagca
    29941 gcgtcgacag agcgatcgag aagcaaccat cggcgtcacg gacagccttg tacatctttt
    30001 tgccaaccgt tgggcgccgc tggttgtcgg gcgcaacatt gggctgatga cgatggaatt
    30061 attcaccccg gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg cgcgttgaag
    30121 gcggttttaa ggaataaaca atgcaaagtg ttgatgtagc cattgttggc ggcggcatgg
    30181 tggggctggc ggttgcctgc ggcttacagg gtagcggctt acgcgttgcc gtactggagc
    30241 agcgcgtacc ggaacctctg gcggcggatg caccaccaca actgcgcgtt tcggctatca
    30301 atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt ctctctcgcc
    30361 gagccagctg ttatcacggt atggaagtgt gggacaaaga cagctttggt cacatttcgt
    30421 ttgacgatca aagcatgggc tatagccatc ttgggcatat cgttgaaaac tcagtgattc
    30481 actacgcgct gtggaacaaa gcgcagcagt cgtcagatat cactctgttg gcccccgcag
    30541 aattacagca ggtcgcctgg ggagaaaacg aaaccttcct gacgctgaaa gatggcagta
    30601 tgttaacggc gcgtctggtg attggcgcgg acggcgctaa ttcctggttg cgcaacaaag
    30661 ccgatattcc gctgactttc tgggattatc agcatcacgc gctggtagcg accatacgca
    30721 cggaagaacc gcatgatgcg gtggcgcggc aggttttcca cggcgaaggc attctggcct
    30781 ttttaccgct tagcgatccg catctttgct cgattgtctg gtcgctgtcg ccagaggaag
    30841 cgcagcggat gcagcaggca ggggaagacg aatttaatcg cgcgttaaat atcgcttttg
    30901 ataatcgcct gggcttatgc aaggttgaga gcgagcgtca ggtgttcccg ctgacggggc
    30961 gttatgcgcg ccagtttgcc gcacatcgtc tggcgctggt gggcgacgcc gcgcatacca
    31021 ttcacccact ggcggggcag ggcgttaatc tcggctttat ggacgccgca gagctgattg
    31081 ctgaactgaa acggttgcat cgtcagggta aagacatcgg gcagtacatt tacctgcgcc
    31141 gctatgaacg tagccgcaag cacagcgcgg cgctgatgct ggctggtatg cagggattcc
    31201 gcgatctgtt ttccggtacc aatccggcga aaaaactgct gcgtgatatt ggtttgaaac
    31261 tggccgacac gcttcctggc gttaagccac aacttatccg tcaggcaatg ggattaaacg
    31321 atttgcctga atggctgcgt taaaaatttc tcttctgctg tttatttgat acgcatcaca
    31381 ctttcatctc ccggtttttt cgccgggaga ttttcctcat ttgaaataaa ctaatttc
//
LOCUS       DAZUCE010000031        31237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZUCE010000031 DAZUCE010000000
VERSION     DAZUCE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.031"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..591
                     /locus_tag="ACHY03_001250"
     CDS             <1..591
                     /locus_tag="ACHY03_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFS3904507.1"
                     /translation="YIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEE
                     VEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIK
                     PHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIK
                     MVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            821..1204
                     /gene="secE"
                     /locus_tag="ACHY03_001251"
     CDS             821..1204
                     /gene="secE"
                     /locus_tag="ACHY03_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFS3904508.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1206..1751
                     /gene="nusG"
                     /locus_tag="ACHY03_001252"
     CDS             1206..1751
                     /gene="nusG"
                     /locus_tag="ACHY03_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFS3904509.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1910..2338
                     /gene="rplK"
                     /locus_tag="ACHY03_001253"
     CDS             1910..2338
                     /gene="rplK"
                     /locus_tag="ACHY03_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFS3904510.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2342..3046
                     /gene="rplA"
                     /locus_tag="ACHY03_001254"
     CDS             2342..3046
                     /gene="rplA"
                     /locus_tag="ACHY03_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFS3904511.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3338..3835
                     /gene="rplJ"
                     /locus_tag="ACHY03_001255"
     CDS             3338..3835
                     /gene="rplJ"
                     /locus_tag="ACHY03_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFS3904512.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3902..4267
                     /gene="rplL"
                     /locus_tag="ACHY03_001256"
     CDS             3902..4267
                     /gene="rplL"
                     /locus_tag="ACHY03_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFS3904513.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4587..8615
                     /gene="rpoB"
                     /locus_tag="ACHY03_001257"
     CDS             4587..8615
                     /gene="rpoB"
                     /locus_tag="ACHY03_001257"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFS3904514.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8692..12915
                     /gene="rpoC"
                     /locus_tag="ACHY03_001258"
     CDS             8692..12915
                     /gene="rpoC"
                     /locus_tag="ACHY03_001258"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFS3904515.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13128..13643
                     /gene="yjaZ"
                     /locus_tag="ACHY03_001259"
     CDS             13128..13643
                     /gene="yjaZ"
                     /locus_tag="ACHY03_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFS3904516.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTICLQLIEK"
     gene            complement(14078..15211)
                     /gene="thiH"
                     /locus_tag="ACHY03_001260"
     CDS             complement(14078..15211)
                     /gene="thiH"
                     /locus_tag="ACHY03_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFS3904517.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15208..15978)
                     /gene="thiG"
                     /locus_tag="ACHY03_001261"
     CDS             complement(15208..15978)
                     /gene="thiG"
                     /locus_tag="ACHY03_001261"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFS3904518.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15980..16180)
                     /gene="thiS"
                     /locus_tag="ACHY03_001262"
     CDS             complement(15980..16180)
                     /gene="thiS"
                     /locus_tag="ACHY03_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFS3904519.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINHQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(16164..16919)
                     /gene="thiF"
                     /locus_tag="ACHY03_001263"
     CDS             complement(16164..16919)
                     /gene="thiF"
                     /locus_tag="ACHY03_001263"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFS3904520.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16912..17547)
                     /gene="thiE"
                     /locus_tag="ACHY03_001264"
     CDS             complement(16912..17547)
                     /gene="thiE"
                     /locus_tag="ACHY03_001264"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFS3904521.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVHWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWWLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17547..19442)
                     /gene="thiC"
                     /locus_tag="ACHY03_001265"
     CDS             complement(17547..19442)
                     /gene="thiC"
                     /locus_tag="ACHY03_001265"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFS3904522.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19523..19623)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19675..20151)
                     /gene="rsd"
                     /locus_tag="ACHY03_001266"
     CDS             complement(19675..20151)
                     /gene="rsd"
                     /locus_tag="ACHY03_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFS3904523.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20246..21019
                     /gene="nudC"
                     /locus_tag="ACHY03_001267"
     CDS             20246..21019
                     /gene="nudC"
                     /locus_tag="ACHY03_001267"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFS3904524.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21059..22123
                     /gene="hemE"
                     /locus_tag="ACHY03_001268"
     CDS             21059..22123
                     /gene="hemE"
                     /locus_tag="ACHY03_001268"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFS3904525.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22133..22804
                     /gene="nfi"
                     /locus_tag="ACHY03_001269"
     CDS             22133..22804
                     /gene="nfi"
                     /locus_tag="ACHY03_001269"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFS3904526.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22847..23437
                     /gene="yjaG"
                     /locus_tag="ACHY03_001270"
     CDS             22847..23437
                     /gene="yjaG"
                     /locus_tag="ACHY03_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFS3904527.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23624..23896
                     /gene="hupA"
                     /locus_tag="ACHY03_001271"
     CDS             23624..23896
                     /gene="hupA"
                     /locus_tag="ACHY03_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFS3904528.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23909..24604
                     /gene="yjaH"
                     /locus_tag="ACHY03_001272"
     CDS             23909..24604
                     /gene="yjaH"
                     /locus_tag="ACHY03_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFS3904529.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24606..25031)
                     /gene="zraP"
                     /locus_tag="ACHY03_001273"
     CDS             complement(24606..25031)
                     /gene="zraP"
                     /locus_tag="ACHY03_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFS3904530.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25269..26645
                     /gene="zraS"
                     /locus_tag="ACHY03_001274"
     CDS             25269..26645
                     /gene="zraS"
                     /locus_tag="ACHY03_001274"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFS3904531.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26642..27967
                     /gene="zraR"
                     /locus_tag="ACHY03_001275"
     CDS             26642..27967
                     /gene="zraR"
                     /locus_tag="ACHY03_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFS3904532.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27964..29253)
                     /gene="purD"
                     /locus_tag="ACHY03_001276"
     CDS             complement(27964..29253)
                     /gene="purD"
                     /locus_tag="ACHY03_001276"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFS3904533.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29265..30854)
                     /gene="purH"
                     /locus_tag="ACHY03_001277"
     CDS             complement(29265..30854)
                     /gene="purH"
                     /locus_tag="ACHY03_001277"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFS3904534.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7363 a         8091 c         8545 g         7238 t
ORIGIN      
        1 tacattccgg aaccagagcg tgcgattgac aagccgttcc tgctgccgat cgaagacgta
       61 ttctccatct ccggtcgtgg taccgttgtt accggtcgtg tagaacgcgg tatcatcaaa
      121 gttggtgaag aagttgaaat cgttggtatc aaagagactc agaagtctac ctgtactggc
      181 gttgaaatgt tccgcaaact gctggacgaa ggccgtgctg gtgagaacgt aggtgttctg
      241 ctgcgtggta tcaaacgtga agaaatcgaa cgtggtcagg tactggctaa gccgggcacc
      301 atcaagccgc acaccaagtt cgaatctgaa gtgtacattc tgtccaaaga tgaaggcggc
      361 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
      421 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      481 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      541 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      601 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      661 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      721 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      781 gggtgaaaat gtttgtagaa tacttctgac aggttggttt atgagtgcga ataccgaagc
      841 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      901 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      961 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
     1021 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
     1081 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
     1141 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
     1201 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
     1261 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
     1321 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1381 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1441 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1501 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1561 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1621 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1681 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1741 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgcgagat
     1801 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1861 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1921 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1981 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     2041 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     2101 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     2161 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     2221 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     2281 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     2341 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2401 gtacgacatc aacgaagcta tcgctctgct gaaagagctg gcgactgcta aattcgtaga
     2461 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2521 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2581 gggtgcaaac gctgaagctg ctaaagctgc aggtgctgaa ctggtaggta tggaagatct
     2641 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2701 aatgcgcgtt gttggccagc tgggccaggt tctgggcccg cgcggcctga tgccaaaccc
     2761 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2821 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2881 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2941 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     3001 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     3061 cggtgatttt gtctacaatc ttacccccac gtatgctaac gaaagttagc agctaaaaag
     3121 atttgttcgt tggagcctgg cctatccagg cctccgtcga agaccgcagg agtttcgcaa
     3181 gaaacttaat cccctgcgta gacggtgaca gaacgctaag attattcttc tatattctgg
     3241 cttgtttctg ctcaccgtaa ttaagacgct ctctccgttt ggaggagtga agtgagttcc
     3301 agagattttc tctggcaaac atccaggagc aaagctaatg gctttaaatc ttcaagacaa
     3361 acaagcgatt gttgctgaag tcagcgaagt agccaaaggc gcgctgtctg cagtagttgc
     3421 ggattcccgt ggcgtaactg tagataaaat gactgaactg cgtaaagcag gtcgcgaagc
     3481 tggcgtatac atgcgtgttg ttcgtaacac cctgctgcgc cgtgctgttg aaggtactcc
     3541 gttcgagtgc ctgaaagacg cgtttgttgg tccgaccctg attgcatact ctatggaaca
     3601 cccgggcgct gctgctcgtc tgttcaaaga gttcgcgaaa gcgaatgcaa aatttgaggt
     3661 caaagccgct gcctttgaag gtgagctgat cccggcgtct cagatcgacc gcctggcaac
     3721 tctgccgacc tacgaagaag caattgcacg cctgatggca accatgaaag aagcttcggc
     3781 tggcaaactg gttcgtactc tggctgctgt acgcgatgcg aaagaggctg cttaatcgca
     3841 gttatctttt taacgcattc gcttacgtat aaacttattc tgatattcag gaacaattta
     3901 aatgtctatc actaaagatc aaatcattga agcagttgca gctatgtctg taatggacgt
     3961 tgtagaactg atctctgcaa tggaagaaaa attcggtgtt tccgctgctg ctgctgtagc
     4021 tgtagctgct ggcccggttg aagctgctga agaaaaaact gaattcgacg taattctgaa
     4081 agctgctggc gctaacaaag ttgctgttat caaagcagta cgtggcgcaa ctggcctggg
     4141 tctgaaagaa gctaaagacc tggtagaatc tgcaccggct gctctgaaag aaggcgtgag
     4201 caaagacgac gcagaagcac tgaaaaaagc tctggaagaa gctggcgctg aagttgaagt
     4261 taaataagcc aacccttccg gttgcagcct gagaaatcag gctgatggct ggtgactttt
     4321 tagtcaccag cctttttgcg ctgtaaggcg ccagtagcgt ttcacactgt ttgactactg
     4381 ctgtgccttt caatgcttgt ttctatcgac gacttaatat actgcgacag gacgtccgtt
     4441 ctgtgtaaat cgcaatgaaa tggtttaagc gtgatagcaa caggcattgc ggaaagtgtt
     4501 ccattttccg gtcaacaaaa tagtgttgca caaactgtcc gctcaatgga cagatgggtc
     4561 gacttgtcag cgagctgagg aaccctatgg tttactccta taccgagaaa aaacgtattc
     4621 gtaaggattt tggtaaacgt ccacaagttc tggatgtacc ttatctcctt tctatccagc
     4681 ttgactcgtt tcagaaattt atcgagcaag atcctgaagg gcagtatggt ctggaagctg
     4741 ctttccgttc cgtattcccg attcagagct acagcggtaa ttccgagctg caatacgtca
     4801 gctaccgcct tggcgaaccg gtgtttgacg tccaggaatg tcaaatccgt ggcgtgacct
     4861 attccgcacc gctgcgcgtt aaactgcgtc tggtgatcta tgagcgcgaa gcgccggaag
     4921 gcaccgtaaa agacattaaa gaacaagaag tctacatggg cgaaattccg ctcatgacag
     4981 acaacggtac ctttgttatc aacggtactg agcgtgttat cgtttcccag ctgcaccgta
     5041 gtccgggcgt cttctttgac tccgacaaag gtaaaaccca ctcttcgggt aaagtgctgt
     5101 ataacgcgcg catcatccct taccgtggtt cctggctgga cttcgaattc gatccgaagg
     5161 acaacctgtt cgtacgtatc gaccgtcgcc gtaaactgcc tgcgaccatc attctgcgcg
     5221 ccctgaacta caccacagag cagatcctcg acctgttctt tgaaaaagtt atctttgaaa
     5281 tccgtgataa caagctgcag atggaactgg tgccggaacg cctgcgtggt gaaaccgcat
     5341 cttttgacat cgaagctaac ggtaaagtgt acgtagaaaa aggccgccgt atcactgcgc
     5401 gccacattcg ccagctggaa aaagacgacg tcaaactgat cgaagtcccg gttgagtaca
     5461 tcgcaggtaa agtggttgct aaagactata ttgatgagtc taccggcgag ctgatctgcg
     5521 cagcgaacat ggagctgagc ctggatctgc tggctaagct gagccagtct ggtcacaagc
     5581 gtatcgaaac gctgttcacc aacgacctgg atcacggccc gtatatctct gaaaccttac
     5641 gtgtcgaccc aactaacgac cgtctgagcg cactggtaga aatctaccgc atgatgcgcc
     5701 ctggcgagcc gccgactcgt gaagcagctg aaagcctgtt cgagaacctg ttcttctctg
     5761 aagaccgtta tgacttgtct gcggttggtc gtatgaagtt caaccgttct ctgctgcgcg
     5821 aagaaatcga aggttccggt atcctgagca aagacgacat cattgatgtt atgaaaaagc
     5881 tcatcgatat ccgtaacggt aaaggtgaag tcgatgatat cgaccacctc ggcaaccgtc
     5941 gtatccgttc cgttggcgaa atggcggaaa accagttccg cgttggcctg gtacgtgtag
     6001 agcgtgcggt gaaagagcgt ctgtctctgg gcgatctgga taccctgatg cctcaggata
     6061 tgatcaacgc caagccgatt tccgcagcag tgaaagagtt cttcggttcc agccagctgt
     6121 ctcagtttat ggaccagaac aacccgctgt ctgagattac gcacaaacgt cgtatctccg
     6181 cactcggccc aggcggtctg acccgtgaac gtgcaggctt cgaagttcga gacgtacacc
     6241 cgactcacta cggtcgcgta tgtccaatcg aaacccctga aggtccgaac atcggtctga
     6301 tcaactctct gtccgtgtac gcacagacta acgaatacgg cttccttgag actccgtatc
     6361 gtaaagtgac tgacggtgtt gtaactgacg aaattcacta cctgtctgct atcgaagaag
     6421 gcaactacgt tatcgcccag gcgaactcca acctggatga agaaggccac ttcgtagaag
     6481 acctggtaac ttgccgtagc aaaggcgaat ccagcttgtt cagccgcgac caggttgact
     6541 acatggacgt atccacccag caggtggtat ccgtcggtgc gtccctgatc ccgttcctgg
     6601 aacacgatga cgccaaccgt gcattgatgg gtgcgaacat gcaacgtcag gccgttccga
     6661 ctctgcgcgc tgataagccg ctggttggta ctggtatgga acgtgctgtt gccgttgact
     6721 ccggtgtaac tgcggtagct aaacgtggtg gtgtcgttca gtacgtggat gcttcccgta
     6781 tcgttatcaa agttaacgaa gacgagatgt atccgggtga agcaggtatc gacatctaca
     6841 acctgaccaa atacacccgt tctaaccaga acacctgtat caaccagatg ccgtgtgtgt
     6901 ctctgggtga accggttgaa cgtggcgacg tgctggcaga cggtccgtcc accgacctcg
     6961 gtgaactggc gcttggtcag aacatgcgcg tagcgttcat gccgtggaat ggttacaact
     7021 tcgaagactc catcctcgta tccgagcgtg ttgttcagga agaccgtttc accaccatcc
     7081 acattcagga actggcgtgt gtgtcccgtg acaccaagct ggggccggaa gagatcaccg
     7141 ctgacatccc gaacgtgggt gaagctgcgc tctccaaact ggatgaatcc ggtatcgttt
     7201 acattggtgc ggaagtgacc ggtggcgaca ttctggttgg taaggtaacg ccgaaaggtg
     7261 aaactcagct gaccccagaa gaaaaactgc tgcgtgcgat cttcggtgag aaagcctctg
     7321 acgttaaaga ctcttctctg cgcgtaccaa acggtgtatc cggtacggtt atcgacgttc
     7381 aggtctttac tcgcgatggc gtagaaaaag acaaacgtgc gctggaaatc gaagaaatgc
     7441 agctcaaaca ggcgaagaaa gacctgtctg aagaactgca gatcctcgaa gcgggtctgt
     7501 tcagccgtat ccgtgctgtg ctggtagccg gtggcgttga agctgagaag ctcgacaaac
     7561 tgccgcgcga tcgctggctg gagctgggcc tgaccgacga agagaaacaa aatcagctgg
     7621 aacagctggc tgagcagtat gacgaactga aacacgagtt cgagaagaaa ctcgaagcga
     7681 aacgccgcaa aatcacccag ggcgacgatc tggcaccggg cgtgctgaag attgttaagg
     7741 tatatctggc ggttaaacgc cgtatccagc ctggtgacaa gatggcaggt cgtcacggta
     7801 acaagggtgt aatttctaag atcaacccga tcgaagatat gccttacgat gaaaacggta
     7861 cgccggtaga catcgtactg aacccgctgg gcgtaccgtc tcgtatgaac atcggtcaga
     7921 tcctcgaaac ccacttgggt atggctgcga aaggtatcgg cgacaagatc aacgccatgc
     7981 tgaaacagca gcaagaagtc gcgaaactgc gcgaattcat ccagcgtgcg tacgatctgg
     8041 gcgctgacgt tcgtcagaaa gttgacctga gtaccttcag cgatgaagaa gttatgcgtc
     8101 tggctgaaaa cctgcgcaaa ggtatgccaa tcgcaacgcc ggtgttcgac ggtgcgaagg
     8161 aagcagaaat taaagagctg ctgaaacttg gcgacctgcc gacttccggt cagatccgcc
     8221 tgtacgatgg tcgcactggt gaacagttcg agcgtccggt aaccgttggt tacatgtaca
     8281 tgctgaaact gaaccacctg gtcgacgaca agatgcacgc gcgttccacc ggttcttaca
     8341 gcctggttac tcagcagccg ctgggtggta aggcacagtt cggtggtcag cgtttcgggg
     8401 agatggaagt gtgggcgctg gaagcatacg gcgcagcata caccctgcag gaaatgctca
     8461 ccgttaagtc tgatgacgtg aacggtcgta ctaagatgta taaaaacatc gtggacggca
     8521 accatcagat ggagccgggc atgccagaat ccttcaacgt attgttgaaa gagattcgtt
     8581 cgctgggtat caacatcgaa ctggaagacg agtaattctc gctcaaacag gtcactgctg
     8641 tcggggtaaa acccggcagc ggattgtgct aactccgacg ggagcaaatc cgtgaaagat
     8701 ttattaaagt ttctgaaagc gcagactaaa accgaagagt ttgatgcgat caaaattgct
     8761 ctggcttcgc cagacatgat ccgttcatgg tctttcggtg aagttaaaaa gccggaaacc
     8821 atcaactacc gtacgttcaa accagaacgt gacggccttt tctgcgcccg tatctttggg
     8881 ccggtaaaag attacgagtg cctgtgcggt aagtacaagc gcctgaaaca ccgtggcgtc
     8941 atctgtgaga agtgcggcgt tgaagtgacc cagactaaag tacgccgtga gcgtatgggc
     9001 cacatcgaac tggcttcccc gactgcgcac atctggttcc tgaaatcgct gccgtcccgt
     9061 atcggcctgc tgctcgatat gccgctgcgc gatattgaac gcgtactgta cttcgaatcc
     9121 tatgtggtta tcgaaggcgg tatgaccaac ctggaacgtc agcagatcct gactgaagag
     9181 cagtatctgg acgcgctgga agagttcggt gacgaattcg acgcgaaaat gggtgcggaa
     9241 gcaatccagg ccctgctgaa gagcatggat ctggagcaag agtgcgaaca gctgcgtgaa
     9301 gagctgaacg aaaccaactc cgaaaccaag cgtaaaaagc tgaccaagcg tatcaaactg
     9361 ctggaagcgt tcgttcagtc tggtaacaaa ccagagtgga tgatcctgac cgttctgccg
     9421 gtactgccgc cagatctgcg tccgctggtt ccgctggatg gtggtcgttt cgcgacttct
     9481 gacctgaacg atctgtatcg tcgcgtcatt aaccgtaaca accgtctgaa acgtctgctg
     9541 gatctggctg cgccggacat catcgtacgt aacgaaaaac gtatgctgca ggaagcggta
     9601 gacgctctgc tggataacgg tcgtcgcggt cgtgcgatca ccggttctaa caagcgtcct
     9661 ctgaaatctt tggccgacat gatcaaaggt aaacagggtc gtttccgtca gaacctgctc
     9721 ggtaagcgtg ttgactactc cggtcgttct gtaatcaccg taggtccata cctgcgtctg
     9781 catcagtgcg gtctgccgaa gaaaatggcg ctggagctgt tcaaaccgtt catctacggc
     9841 aagctggaac tgcgtggtct tgctaccacc attaaagctg cgaagaaaat ggttgagcgc
     9901 gaagaagctg tcgtttggga tatcctggac gaagttatcc gcgaacaccc ggtactgctg
     9961 aaccgtgcac cgactctgca ccgtctgggt atccaggcat ttgaaccggt actgatcgaa
    10021 ggtaaagcta tccagctgca cccgctggtt tgtgcggcat ataacgccga cttcgatggt
    10081 gaccagatgg ctgttcacgt accgctgacg ctggaagccc agctggaagc gcgtgcgctg
    10141 atgatgtcta ccaacaacat cctgtccccg gcgaacggtg aaccaatcat cgttccgtct
    10201 caggacgttg tattgggtct gtactacatg acccgtgact gtgttaacgc caaaggcgaa
    10261 ggcatggtgc tgactggccc gaaagaagca gaacgtctgt atcgctctgg tctggcttct
    10321 ctgcatgcgc gcgttaaagt gcgtatcacc gagtatgaaa aagatgctaa cggtgaatta
    10381 gtagcgaaaa ccagcctgaa agacacgact gttggccgtg ccattctgtg gatgattgta
    10441 ccgaaaggtc tgccttactc catcgtcaac caggcgctgg gtaaaaaagc aatctccaaa
    10501 atgctgaaca cctgctaccg cattctcggt ctgaaaccga ccgttatttt tgcggaccag
    10561 atcatgtaca ccggcttcgc ctatgcagcg cgttctggtg catctgttgg tatcgatgac
    10621 atggtcatcc cggagaagaa acacgaaatc atctccgagg cagaagcaga agttgctgaa
    10681 attcaggagc agttccagtc tggtctggta actgcgggcg aacgctacaa caaagttatc
    10741 gatatctggg ctgcggcgaa cgatcgtgta tccaaagcga tgatggataa cctgcaaact
    10801 gaaaccgtta ttaaccgtga cggtcaggaa gagaagcagg tttccttcaa cagcatctac
    10861 atgatggccg actccggtgc gcgtggttct gcggcacaga ttcgtcagct tgctggtatg
    10921 cgtggtctga tggcgaagcc ggatggctcc atcatcgaaa cgccaatcac cgcgaacttc
    10981 cgtgaaggtc tgaacgtact ccagtacttc atctccaccc acggtgctcg taaaggtctg
    11041 gcggataccg cactgaaaac tgcgaactcc ggttacctga ctcgtcgtct ggttgacgtg
    11101 gcgcaggacc tggtggttac cgaagacgat tgtggtaccc atgaaggtat catgatgact
    11161 ccggttatcg aaggtggtga cgttaaagag ccgctgcgcg atcgcgtact gggtcgtgta
    11221 actgctgaag acgttctgaa gccgggtact gctgatatcc tcgttccgcg caacacgctg
    11281 ctgcacgaac agtggtgtga cctgctggaa gagaactctg tcgacgcggt taaagtacgt
    11341 tctgttgtat cttgtgacac cgactttggt gtatgtgcgc actgctacgg tcgtgacctg
    11401 gcgcgtggcc acatcatcaa caagggtgaa gcaatcggtg ttatcgcggc acagtccatc
    11461 ggtgaaccgg gtacacagct gaccatgcgt acgttccaca tcggtggtgc ggcatctcgt
    11521 gcggctgctg aatccagcat ccaggtgaaa aacaaaggta gcatcaagct cagcaacgtg
    11581 aagtcggttg tgaactccag cggtaaactg gttatcactt cccgtaacac cgaactgaaa
    11641 ctgatcgacg aattcggtcg taccaaagaa agctacaaag taccttacgg tgcggtactg
    11701 gcaaaaggcg atggcgaaca ggttgctggc ggcgaaaccg ttgcaaactg ggacccgcac
    11761 accatgccgg ttatcaccga agtaagcggt tttgtacgct ttactgacat gatcgacggc
    11821 cagaccatta ctcgtcagac cgacgaactg accggtctgt cttcgctggt ggttctggat
    11881 tccgcagaac gtaccgcagg tggtaaagat ctgcgtccgg cactgaaaat cgttgatgct
    11941 cagggtaacg acgttctgat cccaggtacc gatatgcctg cgcagtactt cctgccgggt
    12001 aaagcgattg ttcagctgga agatggcgta cagatcagct ctggtgacac cctggcgcgt
    12061 attccgcagg aatccggcgg taccaaggac atcaccggtg gtctgccgcg cgttgcggat
    12121 ctgttcgaag cacgtcgtcc gaaagagccg gcaatcctgg ctgaaatcag cggtatcgtt
    12181 tccttcggta aagaaaccaa aggtaaacgt cgtctggtta tcaccccggt agacggtagc
    12241 gatccgtacg aagagatgat tccgaaatgg cgtcagctca acgtgttcga aggtgaacgt
    12301 gtagaacgtg gtgacgtaat ttccgacggt ccggaagcgc cgcacgacat tctgcgtctg
    12361 cgtggtgttc atgctgtgac tcgttacatc gttaacgaag tacaggacgt ataccgtctg
    12421 cagggcgtta agattaacga taaacacatc gaagttatcg ttcgtcagat gctgcgtaaa
    12481 gctaccatcg ttaacgcggg cagctccgac ttcctggaag gcgaacaggt tgaatactct
    12541 cgcgtcaaga tcgcaaaccg cgaactggaa gcgaacggca aagtgggtgc aacttactcc
    12601 cgcgatctgc tgggtatcac caaagcgtct ctggcaaccg agtccttcat ctccgcggca
    12661 tcgttccagg agaccactcg cgtgctgacc gaagcagccg ttgcgggcaa acgcgacgaa
    12721 ctgcgcggcc tgaaagagaa cgttatcgtg ggtcgtctga tccctgcagg taccggttac
    12781 gcgtaccacc aggatcgtat gcgtcgccgt gctgcgggtg aagctccggc tgcaccgcag
    12841 gtgactgcag aagacgcatc tgccagcctg gcagaactgc tgaacgcagg tctgggcggt
    12901 tctgataacg agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt
    12961 ttatgggggg agtttaggga aagagcattt gtcagaatat ttaaggaatt tctgaatact
    13021 cataatcaat gtagagatga ctaatatcct gaaactgact gaactaattg agtcaaactc
    13081 ggcaaggatt cgatactatt cctgtgtaac tttcttaagg aacgagaatg aaacaggaag
    13141 tggaaaagtg gcgacctttt ggacatccgg atggtgatat tcgtgattta tcatttcttg
    13201 atgctcatca ggctgtctac gttcagcatc atgagggcaa agagccttta gagtatcgct
    13261 tttgggttac ctactctctt cactgcttca caaaagatta tgaacatcag acgaacgaag
    13321 aaaaacaatc gttaatgtac cacgcgccta aagaatctcg tcccttctgc cagcaccgtt
    13381 ataacttagc gcgcacacac ttaaaaagaa ctattttggc gctgccagaa agcaacgtta
    13441 ttcatgccgg gtatggtagc tatgccgtga ttgaggtgga cttagacgga ggagataagg
    13501 cattttactt tgttgcgttc agggctttca gggaaaagaa aaaactccgt ttgcatgtaa
    13561 ctagcgctta tcccatttct gaaaaacaga aaggtaaatc agtgaaattt tttaccattt
    13621 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13681 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13741 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13801 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13861 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13921 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13981 gacggcacta ccataaaaaa acacaacgct ttcaacaacc ttgcgttttt catcttccgt
    14041 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc
    14101 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    14161 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattgt
    14221 cggcatagcc acctggctgc gttttcgaaa aagcgctgac gttattaatt gccagcggaa
    14281 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14341 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14401 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14461 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14521 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cggcccagcc
    14581 gatccggcgt ttccagccgc cagaagaagt cctgtttttt gcctttcaga tgatggcggg
    14641 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagttgct
    14701 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catctgtagt gaagagaact
    14761 gttcacgcag ggcagggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14821 caccggtgac taaaagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14881 caatatccgc ttcgtccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14941 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    15001 tgccaaaacg ctgacgggtc aggcgctgcg cccgttgggc cagtggttcc agatagccac
    15061 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg
    15121 cccgctctac gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca
    15181 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cggtcagcgg
    15241 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15301 tgcttctact gccagacgaa atgccttcgc catgttgacg ggatcgtccg cgacggcaat
    15361 cgccgtattc actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg
    15421 aacgccgatg ccagcatcga caaccaccgg aactgtggcc tgctggataa taatctccag
    15481 catggcacgg gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15541 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15601 cacgacaaat ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcagg
    15661 caatagccag cgggcgtcag ggtgaatctc taattttagc cagtttgtgc ctaatgcttc
    15721 acgtgccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15781 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15841 gtcgacacgt ttcatcgcca gcgtcaccag ttggctgccg gaagcgcgaa tagactccac
    15901 catcagttgt gaagaggcga atttcccggt gccggtaaac agatgtgaat caaacgtttt
    15961 gtccgcaata cgtaacatct caaccccctg caataacctg aaaaagcagg atctggtcgc
    16021 catcctgcac gatatgttgc gtccactgct cacgcgggac gatttgatga ttaatcgcca
    16081 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    16141 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    16201 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgacttacc gtcgaacagt
    16261 cggagttctc ccgcaggcgt ctctataccg cttaataact taatggcttc tagtgcctgc
    16321 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16381 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16441 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16501 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16561 tcggcttgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16621 agttgaatat cgggattcaa ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga
    16681 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16741 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtatagcgc agcaggtgta
    16801 cccagcccgc caagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtca
    16861 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16921 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt
    16981 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    17041 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    17101 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    17161 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    17221 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17281 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc accagtaatc
    17341 gttgataaac aatcgcgcgt tatagcggcg gcccagtgca attgccgcca cgacatcggc
    17401 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17461 caacagacgt tcgatccaat gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17521 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17581 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17641 gcgtaatcac gcacttcctg gctgattttc atcgagcaga atttcggccc acacatggag
    17701 caaaaatggg cgactttacc tgactcttgc ggcagggttt catcgtgata agcgcgggcg
    17761 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17821 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17881 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17941 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    18001 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    18061 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    18121 tgcatcggca cgtggcctgg gccttcaatc atcacctgca catcatattc ccaggcgatt
    18181 tttgtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18241 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18301 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18361 ttcgccataa tcgaaccgcc gcgcgagacg ataccggtca ggcgtttcgc ggtcatcggc
    18421 acatagcgca gcagcacccc tgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18481 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18541 tccagcgcct ggtagatcgg cactgtaccg atcggcactg ggctgttacg caaaatccac
    18601 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18661 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgctgagttg
    18721 ccgatattgg cgttaacttt taccaggaag ttacggccaa taatcatcgg ctccgattcc
    18781 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18841 tccgcagtga tattttctgg cagacgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18901 aaaacttcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18961 tccggtgtga tgatgccctg gcgggcgtag tgcagttggg tgacgcggcg tcctgttttg
    19021 gcgcgttttg gcgttaataa gccgctaaaa cgtagctcgt cgaggccatc atctgccagc
    19081 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    19141 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcgat ctgcggatcg
    19201 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19261 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19321 ccgggttgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19381 gtgtcgataa aatgttgggc ctgggcgcgt tgttcgcgac gggtcagttt tgttgcagac
    19441 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19501 atcgccgtaa aggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19561 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19621 attagcccca caaacggggc gttgaatgta aattacgcgt taacagcgca gaactcaagc
    19681 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19741 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19801 atgatcgata gcggtttcca ggctggaatc gtagtaatcc ataatctgtt gggtattggc
    19861 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19921 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19981 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    20041 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    20101 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    20161 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    20221 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20281 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20341 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20401 acctgtttgg ttaatacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20461 tgatctcgat gttgggctgt ttcaactggc cggacgtggc gtgcaactgg cggagtttta
    20521 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccaagca aaaccgaatg
    20581 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20641 tgttgccatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20701 tgtccatacg gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcggtcgc
    20761 gcgggaagtg atggaagaga gcggcatcaa agttaaaaac ttgcgttatg tgacttccca
    20821 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20881 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20941 gttactcccg ccgcccggca ccgtggcgcg ccgtctgata gaagatacgg tggcgatgtg
    21001 tcgggcagag tacgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    21061 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    21121 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    21181 ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgtg aagtgacctt
    21241 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21301 cccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21361 gccagtcacc tgcaaagctg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21421 ggggtacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21481 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21541 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21601 actcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21661 tcaggctgtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21721 gttctcgctt tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    21781 ccgcgtaccg gtcacgctgt ttaccaaagg cggcggacag tggctggaag ccatggcaga
    21841 aaccggttgc gatgcgctgg gcctcgactg gacaacggac atcgccgatg cgcgccgccg
    21901 tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc
    21961 tgcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    22021 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    22081 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagcga ttatggatct
    22141 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    22201 cgataaggat ccgccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22261 agtgacgcga gcggcgatgg tgctgctgaa atacccctcg cttgagctgg tggagtataa
    22321 agtggcccgc atcgctacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22381 tcctgcgctc ctggcagcgt gggagatgct gtcacaaaaa ccggatttag tgtttgtcga
    22441 tggtcatggg atctcgcatc ctcgccgtct tggcgtcgcc agccattttg gtttgctggt
    22501 ggatgtgccg accattggcg tagcgaaaaa acgactatgc ggtaaattcg aaccgctctc
    22561 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22621 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22681 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22741 ctgggcggac gcggtggcct cggaacgtcc agcgttcgtg cgctatacag caaatcagcc
    22801 ctaattcagg taaactgcgg gtaatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22861 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22921 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggcgatgg
    22981 gcaaatttac cgccgtattc tcgatctcat ctgggaaacg ctgaccgtca aagacgcaaa
    23041 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    23101 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    23161 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    23221 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagctcaa
    23281 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23341 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23401 gggtgagagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23461 ctgatttgtc atacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23521 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23581 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23641 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23701 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23761 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23821 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23881 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23941 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    24001 tagtcacaac tcgtcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    24061 ggtacgtatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    24121 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    24181 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24241 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24301 ccaaatcgat ttataccgtt atcgggctga tcagatccgc cagattagcg atgccttacg
    24361 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24421 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24481 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24541 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24601 ctgatttacc agtggcccat ccccatatga ccgccaccac cgcagccgcc gtagcccatg
    24661 cccattccgg ctccgcgcgg aatacccgct tcagccatcg caatatcccg tttcacccgt
    24721 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24781 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt cactagttgc
    24841 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24901 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24961 gcagatgtcg atcccatcgc cattgctgaa agcgccatca tcaccagggc aattttcgtg
    25021 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    25081 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    25141 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    25201 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25261 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25321 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25381 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25441 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25501 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25561 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25621 cgcaggaaat gcagcagtta catccgggag atgaagaagt gtggcggcgg atcgatagcg
    25681 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25741 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25801 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25861 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25921 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctggtggcgc
    25981 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    26041 gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc
    26101 aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt aagcgagctg ctggaactgg
    26161 ttaagccaac gcatctggca ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    26221 tacagctggt aagccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26281 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26341 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gccagcgaaa
    26401 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26461 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26521 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26581 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26641 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26701 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26761 ggcgttggag caggtgcggg aacaggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26821 ggagatggac ggcatcgcca cgctgaaaga gatcaaagcg ttaaatccgg cgattccggt
    26881 actgattatg accgcgtact ccagcgtcga gacggcggta gaagcgttga aaaccggggc
    26941 gctggattat ctcatcaagc cgctggattt cgataaccta caggcgacgc tggaaaaggc
    27001 gctcgcgcat acgcacagta ttgatgctga aacacctgcg gtgactgcca gccagttcgg
    27061 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    27121 atcggaagcc acggtgctga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    27181 ggcgattcat gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgcggc
    27241 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgttcactgg
    27301 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27361 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27421 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27481 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27541 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aagatatccc
    27601 tctgctggct ggccattttc ttcagcgctt tgccgagcgt aatcgcaagg cggtaaaagg
    27661 ctttacgcca caggcgatgg atttgctgat ccactacgac tggccgggaa atattcgtga
    27721 actggaaaac gcggtggaac gggcggtggt gctgctgacc ggggaatata tttccgaacg
    27781 cgagctgccg ctggcgattg ccggtacgcc gatcccgctg gggcaaagtc aggatattca
    27841 gccgctggtg gaagtcgaaa aagaagtgat tctggcggca ctggagaaaa cgggcggcaa
    27901 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27961 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    28021 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    28081 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgtcatccgc
    28141 cagttttgtg cccgcgtgga acactttgcc gcctgccact tcttccagcg gcaggccgtg
    28201 gatcacatca ccggtgcggt aatcacccgg atatccaccc gcagccatca ccacgccgag
    28261 agaagcgcgt tcatcccact cggatgtttt ctcgtccagc ttgccttcac aggctgccag
    28321 gcagagttca accagatcgg acttcatgcg cagcataatc ggctgggttt ctggatcgcc
    28381 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcagacccgc
    28441 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28501 aatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28561 gcccatcccg ccggtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28621 ggtggccatc ggcaatacat gctcgccgtc caccatcacg ataaagctcg cttcttcgcc
    28681 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28741 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28801 ggcagccaga ccgtcagctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28861 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28921 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28981 gattttcagc ccggcagcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    29041 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    29101 ggtcacgccg atagcaacgt tttgcagcgc gggttccagc gcagtgcctg cattacccgg
    29161 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    29221 gcgcccgccg ttaccaatca ctaatacttt catctattgc tccattaatg gcggaagtgg
    29281 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29341 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29401 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29461 ccttcatcgg cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29521 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29581 ttcgccactt tccagcagaa cagcgcatca cgcagttcct gttcggtcgg ctgacgttgg
    29641 gtgacgacgc gcagttcttc cgcaccgacc atgcccagat cgcgatcctg aaccagcaga
    29701 ccgccgttca cgcgtttgaa atcgaggccc ggaacacgct cgccccactg accgcaggtc
    29761 agaacgcgta cattctgttt ggcggcggtg attttcaggg cttcttcgct ggcggacggc
    29821 gcaataatca cttcaacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatccagc
    29881 tcgcggttaa aggcaatgat gccgccgaat gcggaggtcg ggtcggtttt gtacgcgcga
    29941 tcgtaagcat caagaatgga attgccgata gccacgccgc aagggttggc gtgcttcaca
    30001 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    30061 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    30121 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    30181 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccggc ggcttctttg
    30241 ctttcaccgt ggtaagccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30301 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30361 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30421 gcgacatctt tatggttctt ggcggcggag cgcaccatcg ttgggccgcc gatatcgatg
    30481 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30541 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30601 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30661 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30721 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30781 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30841 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30901 tttcgcaaaa aactcagcta acgcccctaa cggggcatct ttatttttcg cccgcattgt
    30961 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    31021 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    31081 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa
    31141 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    31201 tgaatcactt cacacaaaca gccggttcgg ttgaaga
//
LOCUS       DAZUCE010000032        30706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZUCE010000032 DAZUCE010000000
VERSION     DAZUCE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.032"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            104..1687
                     /gene="yegH"
                     /locus_tag="ACHY03_001278"
     CDS             104..1687
                     /gene="yegH"
                     /locus_tag="ACHY03_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFS3904535.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            1762..2100
                     /locus_tag="ACHY03_001279"
     CDS             1762..2100
                     /locus_tag="ACHY03_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3904536.1"
                     /translation="MRKKLAFLDTSLDDLRAFPESSRQEIGYQLDRIQQGLNPYDWKP
                     FSTIGPGVREIRTRDADGIYRVMYVAKFEEAVYVLHCFQKKTQTTSQSDIDLAKRRYK
                     ELVQERKNEN"
     gene            2090..2380
                     /locus_tag="ACHY03_001280"
     CDS             2090..2380
                     /locus_tag="ACHY03_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3904537.1"
                     /translation="MKIETFDSVWDAVSDTPEQAENMRIRAELVTIINNWIEQQGFSQ
                     AQAASALGVTQPRISELARGKIQIFSIDKLITMMAHAGLHIQRIEVQYPHAA"
     gene            complement(2433..4286)
                     /gene="asmA"
                     /locus_tag="ACHY03_001281"
     CDS             complement(2433..4286)
                     /gene="asmA"
                     /locus_tag="ACHY03_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFS3904538.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGNAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     TAHVEMADTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDILVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4308..4889)
                     /gene="dcd"
                     /locus_tag="ACHY03_001282"
     CDS             complement(4308..4889)
                     /gene="dcd"
                     /locus_tag="ACHY03_001282"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFS3904539.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4981..5622)
                     /gene="udk"
                     /locus_tag="ACHY03_001283"
     CDS             complement(4981..5622)
                     /gene="udk"
                     /locus_tag="ACHY03_001283"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFS3904540.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5940..9257
                     /gene="dgcE"
                     /locus_tag="ACHY03_001284"
     CDS             5940..9257
                     /gene="dgcE"
                     /locus_tag="ACHY03_001284"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS3904541.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWAQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIVSAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMLAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAIFDHAESVQKLRLGGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGEVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9366..10214)
                     /gene="alkA"
                     /locus_tag="ACHY03_001285"
     CDS             complement(9366..10214)
                     /gene="alkA"
                     /locus_tag="ACHY03_001285"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFS3904542.1"
                     /translation="MYTLNWQPPYDWLWMLGFLAARAVNGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRLYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            10348..11700
                     /gene="yegD"
                     /locus_tag="ACHY03_001286"
     CDS             10348..11700
                     /gene="yegD"
                     /locus_tag="ACHY03_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3904543.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAEIRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11713..13653)
                     /gene="yegI"
                     /locus_tag="ACHY03_001287"
     CDS             complement(11713..13653)
                     /gene="yegI"
                     /locus_tag="ACHY03_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFS3904544.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFADSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDSLRQQLKKCTVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGNFVLAKVWAMVMASVAPPAVQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAESRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13650..14411)
                     /gene="pphC"
                     /locus_tag="ACHY03_001288"
     CDS             complement(13650..14411)
                     /gene="pphC"
                     /locus_tag="ACHY03_001288"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFS3904545.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAKLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGRGLELPLTPMVGEYANMTHFI
                     SDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14408..15067)
                     /gene="yegL"
                     /locus_tag="ACHY03_001289"
     CDS             complement(14408..15067)
                     /gene="yegL"
                     /locus_tag="ACHY03_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFS3904546.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(15288..15347)
                     /gene="ibsA"
                     /locus_tag="ACHY03_001290"
     CDS             complement(15288..15347)
                     /gene="ibsA"
                     /locus_tag="ACHY03_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFS3904547.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15621..15677)
                     /locus_tag="ACHY03_001291"
     CDS             complement(15621..15677)
                     /locus_tag="ACHY03_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS3904548.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15949..16005)
                     /locus_tag="ACHY03_001292"
     CDS             complement(15949..16005)
                     /locus_tag="ACHY03_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS3904549.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            16284..17531
                     /gene="mdtA"
                     /locus_tag="ACHY03_001293"
     CDS             16284..17531
                     /gene="mdtA"
                     /locus_tag="ACHY03_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFS3904550.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17531..20653
                     /gene="mdtB"
                     /locus_tag="ACHY03_001294"
     CDS             17531..20653
                     /gene="mdtB"
                     /locus_tag="ACHY03_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFS3904551.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKVFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20654..23731
                     /gene="mdtC"
                     /locus_tag="ACHY03_001295"
     CDS             20654..23731
                     /gene="mdtC"
                     /locus_tag="ACHY03_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFS3904552.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGEAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23732..25147
                     /gene="mdtD"
                     /locus_tag="ACHY03_001296"
     CDS             23732..25147
                     /gene="mdtD"
                     /locus_tag="ACHY03_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3904553.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNHRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            25144..26547
                     /gene="baeS"
                     /locus_tag="ACHY03_001297"
     CDS             25144..26547
                     /gene="baeS"
                     /locus_tag="ACHY03_001297"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFS3904554.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26544..27266
                     /gene="baeR"
                     /locus_tag="ACHY03_001298"
     CDS             26544..27266
                     /gene="baeR"
                     /locus_tag="ACHY03_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFS3904555.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPVVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27457..27789
                     /gene="yegP"
                     /locus_tag="ACHY03_001299"
     CDS             27457..27789
                     /gene="yegP"
                     /locus_tag="ACHY03_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFS3904556.1"
                     /translation="MAGWFELSKSSDSQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27987..28284
                     /locus_tag="ACHY03_001300"
                     /pseudo
     CDS             27987..28284
                     /locus_tag="ACHY03_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
     gene            28286..28582
                     /locus_tag="ACHY03_001301"
     CDS             28286..28582
                     /locus_tag="ACHY03_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3904557.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRTLNQNQDATRHLHWS"
     gene            28685..30046
                     /gene="yegQ"
                     /locus_tag="ACHY03_001302"
     CDS             28685..30046
                     /gene="yegQ"
                     /locus_tag="ACHY03_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFS3904558.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(30376..30693)
                     /gene="yegR"
                     /locus_tag="ACHY03_001303"
     CDS             complement(30376..30693)
                     /gene="yegR"
                     /locus_tag="ACHY03_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFS3904559.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         7098 a         8148 c         8090 g         7370 t
ORIGIN      
        1 aataatgtga gataaatagt gccgcaacat tgcatttttg ccctgattta tccatgatcg
       61 aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat ggattgccga
      121 tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc tcggcattga
      181 taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaagcagc gcgaccgcgc
      241 acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctgg cgtcaatctc
      301 ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct ttagcgcccg
      361 cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga tggagctgaa
      421 cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg cgaagttctg
      481 gggcgtggtg acgcaaattg tggtactgga cgccatcttc tcactcgact cagtgattac
      541 cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta tcgctatcag
      601 cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc cgaccatcgt
      661 tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag aaggtttcgg
      721 cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga tgatcgaggc
      781 gctcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaacc agacgctgcg
      841 ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagagg atgcggaact
      901 ggacgccgaa accgcgtcca tgctggtgga tcatggtaac cagcagatct ttaatccgca
      961 ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtactgtca gcagcattat
     1021 gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag aaatccgcca
     1081 gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg acgcagaaga
     1141 tttgctcggt gttgttcacg ttatcgacct gctacaacag tctctgcgcg gcgaaccgct
     1201 caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc cgttgttacc
     1261 tgccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg atgagtttgg
     1321 ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgccg gtaacttacc
     1381 gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg acggttcctg
     1441 gacggcgaat ggtcatatgc cgctggaaga tctggtgcaa tatgtgccgc tgccgctgga
     1501 tgaaaaacgt gaatatcaca ccattgctgg gctgttgatg gaatatttgc agcgtattcc
     1561 aaaacctggc gaagaagttc aggtagggga ttatttgctt aaaacgttgc aggtggaaag
     1621 ccatcgcgtg cagaaggtgc aaattattcc gctgcgtaag gatggcgaga tggagtacga
     1681 ggtgtgataa aacatcaaat gcgcaggttc aaactgcgca tttgatttat acaaaattta
     1741 gtataatcta aggatggagt aatgagaaaa aagctggctt ttcttgatac cagtcttgac
     1801 gatttacgcg catttccaga atcgtctcga caggaaattg gctatcaact cgacaggatc
     1861 caacaagggt taaacccata cgactggaaa cctttttcaa ctatcggccc gggtgtgcga
     1921 gagattcgta ctcgcgatgc tgatgggatc tatcgcgtca tgtatgtcgc aaaatttgaa
     1981 gaagcggttt acgtattgca ttgttttcaa aagaaaacac agactaccag tcaatcggat
     2041 attgatttag ccaaacgacg ctataaagaa cttgttcagg agcggaaaaa tgaaaattga
     2101 aacctttgat agtgtgtggg atgcggtgag tgacacccct gaacaagcgg aaaatatgcg
     2161 gatccgtgcc gagcttgtaa cgatcattaa taactggatt gaacaacaag ggtttagcca
     2221 ggctcaggca gcttctgctt taggggtcac tcaacctcgc atctctgaac ttgccagagg
     2281 taaaatccag atctttagta tcgataaatt gatcaccatg atggctcatg caggactaca
     2341 tattcaacgt attgaagttc aatatccaca tgctgcttag tgctataaat acagatggcg
     2401 acaaattgat gccgccatct atataacttg tcttacatct tctccagcaa cttcttcaca
     2461 tctttgccat tgcgggaatc tttattccgc tcggcccagt cattcaggcg acgtttcgct
     2521 tcgtcctgta gatgtttgcg cagtaactga tccacttgca gactgtaatt gagttgctgc
     2581 cagttgccat aaacccgcag cggtactggc gtttctttga ggaaatcaat cagtttgctt
     2641 tccccgttcc agccacccac gaccagaata tcaaactggg tgtcgcaggt ttgatctgcc
     2701 agattcaaca tgccttcccc tgtaagcgcc agcacaggcg attgaccttg catgtcgttt
     2761 aacgtcacga cgccatcctt caacgtcaaa tcggtggtaa agcggtcaag acgcgtcacg
     2821 ttatcgaagt tttcagcggc cttcacatca ccaccattac gttctaccgc ttgctgaatc
     2881 atctgctgga agttcatccc ttccatgcgc gtatcggcca tttcgacatg cgccgtcccc
     2941 tgccagttgt ggcggaatgc gtcggcatct atgtcagcac cggagaaatc accagccagt
     3001 gacatttttc cggtcaacga aatcggatag ttaaacgcct tcagaatggt accaatctca
     3061 acgttttcca gccgtggctg gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg
     3121 cccggcagtg aaacctgtcc accgttaagt ttgccctgca gttgagtaat ttccagcaaa
     3181 cccgacttgt tggtcatttg cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg
     3241 ttactggcct gcaacaaaat atcagccgta aagccttgca gtccctgata ggccggttca
     3301 tcaatacgcg aagaaatgac cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg
     3361 ttaccgtttt caccattcgc tgtttcatta agcgggatga ggttgtcaag attcagttgc
     3421 gggaattgca gtctcagctg ccattccggt ttctctgtca gcgtgacctg tgcttgcccg
     3481 ctcagcgtac tgtcattggc ggtcaagctg atttggttaa atgaaaggcg tttatgtgac
     3541 tcctgccact gggcctggaa actcccctgc ccctgaatac cttgttttgg taaatcggca
     3601 ccctgcaact gccagttaat ttgttcaata gccgccgtta aatcatgcgg ataatcagaa
     3661 gcatctaccg taccgttaag ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg
     3721 gagaactcaa atgagccacg atgttgggga tcttgttcca tttgcaggcg aatattgcgg
     3781 attgtcacct gctcgtcatc ttcatgctgg aacaccagaa cgctgtccgc caccttaaga
     3841 ctggatatat caaacgacca tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc
     3901 ggtgcaaccg gagcgtcttc actgcgcacc gcttccgtct gcggcgtcag ttggatcact
     3961 gcccctttta gcatcacctg cttaacgctc agttgatgac tcagtagtgg taaaagcgcc
     4021 acgtccagac gcatgttgtc ggcgcgaacc agtggctggc tcgcgccctg ggcggtgaga
     4081 gacattcgcc cggagaggat actaagctgc ggccagacgt gccaacgcag tggcccgtcg
     4141 agctgcaatt gataaccgct acgtgcagca acttgcttga ccatatagtc gcggaaatca
     4201 ttcggattca ccagcaacac taacgcagat aacccggcca ccagcacgac caggagtatc
     4261 atcagcgtcg tcagaaatcg tctcatggta tcctcaatgg gcctgaatta gtctttatcg
     4321 attcggctgg ctaccgcgcc ctgctggttg cgatacttcg catcttcacg gcggttgtaa
     4381 ggtcgcgccg ccgggccgga aagcggctca aagctcagcg caccaattaa catgcccgga
     4441 cgtagcgcca gcggcagctt accggagttg tagaactcca gcacaatgca gccagaccag
     4501 cccggatcga tgcggtgcgc ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa
     4561 cgcccgtcca gccagcccac cagatcggct ggcagcgtca ccgactccag cgtcaccgcc
     4621 agcgccagct ctcctgggtg aagatagaac gcctcgccct cgtcgagaac gatctcatcg
     4681 ctcatcacgc ggtcaagcgc ggcgctcact tcatctttgg gaccgctcag atcgataaac
     4741 gctgccgtgt gaccacggaa ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg
     4801 ccgttaatac gctccactgg cggacgtggg ttgatcgaca aacggccttc atcaagccag
     4861 gcttcaatat ctcggtcaca cagacgcatg gcattttctc ctttcgcgca tcactccctt
     4921 aacgccaatt acgtcaaggg cataccaggt tatcactgaa cggtacacaa tttatcaagc
     4981 ttattcaaag aactgactta ttttcgcttt caatatatcg atcgcgatgc ggtttttccc
     5041 gccgcgcggc acgataatgt ccgcatattg tttagaaggc tcaatgaatt gcaggaacat
     5101 cggacgcacg gttttttgat attgcgccat cacggaatcc attgaacgcc cacgctcgtt
     5161 aacgtcacgc ttgatgcggc gcatcaggca gatatccagc ggggtatcaa cgaaaatgga
     5221 gaagttaagt tcgtcacgca aacgtgcatc cgtcagcaac aaaatgcctt cgagaatgat
     5281 gaccttcttc ggctcaaccg tcaccgtttc tttcatacgc gtatgttcaa catagctgta
     5341 aaccggcagg tcaattgccg agccgcgttt caacgcttgt aaatgctcaa gcagcaggct
     5401 gtgatccatc gcgctgggat ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg
     5461 gctttgatct ttgtaatagc agtcttcggg aattacgccg atgtgttcat caccgacttg
     5521 ctcacgtaat tcacgataaa gggtactggc aataagactc ttgccggaag ccgatgcgcc
     5581 agcgataccg ataatgacgc actgatgaga ctgatcagtc atatatttag cgacctgatt
     5641 aacctggatg ttaggaaggg ggcgacgaag cgccaaacgc ggcaattata gggatttcat
     5701 ccgcctgata ccagtcgaat agcgttgccg cgcgctcaga gttaattgtt gacgaagaat
     5761 tcccggtggc aaattacgtt gatcagtttt atacaaggta aaaattgtta tacgcagttg
     5821 cgcaaattat ccgcctttac gtcactttat gagcaattcg catataaaat gtaaaacttt
     5881 tgtactagca taaacacaga aacgaatact ggcgacctgg ccttgcggat aaagcggtaa
     5941 tgagcaaaca atcacagcat gtattaattg ccctgcccca cccgctgctt cacctggtca
     6001 gtttaggttt agtctcgttt atcttcaccc ttttctcgct tgagctttcg cagtttggca
     6061 cccaactcgc cccactgtgg ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg
     6121 ccgggcgcat gtggccaggg attgcgctga gctgctcgct gggaaatatc gccgcatcca
     6181 tcctgctttt ttccaccagc tcgctgaaca tgacctggac gaccatcaat attgttgaag
     6241 ccgtggtcgg ggcagtgcta ctacgtaaat tgctgccgtg gtataacccc ttgcaaaatc
     6301 tggctgactg gctgcgtctg gcactcggca gcgccattgt tccgcctctg ttggggggtg
     6361 ttctggttgt cctgctgacg cccggagacg atcctctcag ggcatttttg atatgggtac
     6421 tgtcagaatc catcggcgct ctggcactgg tgccgctggg attgttattt aaaccacact
     6481 atctgctgcg ccatcgcaac ccacggttgc tttttgagtc gctgctcacg ttagccatca
     6541 cactgacgtt aagctggctt tcgatgctgt atctgccgtg gccttttact ttcattattg
     6601 tgctgttgat gtggagcgcc gtgcgcctac cacgaatgga agcctttttg atcttcctta
     6661 ccacggtgat gatggtgtcg ctgatgatgg ccgcggatcc ttccctgctt gctacgccgc
     6721 gtacatacct gatgagccat atgccgtggc taccgttttt gctgatcctg ctgcccgcca
     6781 acatcatgac catggtgatg tatgcctttc gtgcggaacg caaacacatt tccgaaagcg
     6841 aaacccgctt tcgaaacgcg atggaatatt ccgctatcgg catggcgtta gtgggcaccg
     6901 agggacaatg gctgcaatcc aacaaagcac tctgccagtt tctcggttac agtcaggaag
     6961 agttgcgcgg actcaccttt cagcaactga cctggccgga ggatctcaat aaagatctcc
     7021 aacaggttga aaagctgata agcggtgaaa taaacaccta ttcaatggaa aaacgttact
     7081 acaaccgcaa tggcgatgtt gtctgggcgt tgcttgccgt ctcactggtg cgccacacgg
     7141 atggcacgcc gctctatttt atcgctcaga ttgaagacat taacgagcta aaacgcaccg
     7201 aacaggtcaa tcagcaacta atggagcgca tcactctggc taacgaagcg ggagggattg
     7261 gcatctggga gtgggagctg aagccgaata tttttagctg ggataagcgg atgttcgagc
     7321 tgtatgaaat tcctccgcat atcaaaccga actggcaggt gtggtacgag tgcgtgctgc
     7381 cggaagatcg ccagcatgcc gaaaaagtaa ttcgtgattc gttgcaatca cgctcgccct
     7441 ttaaactgga atttcgcatt accgtgaaag acggtattcg ccatatccgc gccctcgcca
     7501 accgggtact gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga
     7561 ccgaggttaa acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc
     7621 ttgattccat cggtgaagcc gtggtctgta ttgatatggc gatgaaaatt acctttatga
     7681 atccggtggc ggagaagatg agcggctggg cgcaggaaga agcattaggt gttccgctcc
     7741 tgacggtgtt gcatattact tttggcgaca acgggccatt aatggagaat atttacagtg
     7801 ccgacacctc acgttccgcg atcgaacaag atgtggtgtt gcactgtcgg agcggcggca
     7861 gttacgacgt gcattacagt attacgccgt taagtactct ggacggcagc aatattggtt
     7921 cggttctggt gattcaggac gttaccgaat cacgcaaaat gctgcgccag ctgagctaca
     7981 gcgcctccca tgatgcactg acgcatctcg ccaaccgcgc cagttttgag aaacaactgc
     8041 gtatcctgct gcaaacggta aacagtacgc atcagcgaca tgcactggtg tttatcgatc
     8101 ttgatcgctt taaagcggtg aatgacagcg ccgggcatgc ggcgggcgac gctttgctgc
     8161 gcgaactggc gtcgttgatg ctgagtatgc tgcgctccag tgacgtgctg gcgcggctcg
     8221 gcggtgatga atttggtctg ctgctgccag actgcaatgt tgaaagcgct cgttttatcg
     8281 ctacacgtat tgtcagtgct gtgaatgact atcactttat atgggaaggc cgggtacatc
     8341 gggtaggtgc cagcgccggg attaccttga tcgatgacaa caatcatcag gcggcagaag
     8401 tgatgtcgca ggctgatatc gcctgttatg cctccaaaaa tggtggccgg ggccgggtga
     8461 cggtttacga accgcagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg
     8521 aacagtggcg gatgattaaa gagaatcagt tgatgatgct cgcccacggt gtcgcttcgc
     8581 cacggatccc ggaagcgcgt aatttgtggc tgatttcact taagctctgg agttgcgaag
     8641 gcgagattat tgatgaacaa acatttcgtc gtagcttcag cgatccggcg cttagccatg
     8701 ctcttgaccg ccgggtattc cacgaatttt tccagcaggc cgcaaaagcg gttgccagta
     8761 aaggcataag catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga
     8821 atgatctgct tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta
     8881 ttccggcaga agcgattttc gatcacgcag aaagcgtgca aaaactgcgg ctggggggat
     8941 gtcggatcgt gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga
     9001 atatggcaga ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga
     9061 tggatgagat gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca
     9121 tcgccgggcc agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc
     9181 tgatttatgg tgaggtgatt gccgatgccc aaccgctgga tttgctggtg aatagtagtt
     9241 atttcgcgat taactgaggc gtgcttcccc atcgcctgat gcgacgctaa cgcgtcttat
     9301 catgcctaca aatcgctcat tccccaggcc ggataaggcg ctcgcaccgc atccggcgac
     9361 caacgttatg cttcgtctgg ttgccagcct tccgtatacc agatatgcaa cagcgcataa
     9421 gaacgccagg gcttccagcg ttcggcataa aggcggattt gtgccggtgt cattcccgga
     9481 aatcgctgtt taatcagata atcatccggc aggaaaacat ctttcgcctg ccagccgcgc
     9541 aaggcgaaat aattcgccgt ccagcgcccg atacccggaa aagtttgcag cgttttcatc
     9601 gcctgctcca catcgcccgg aattgtcatt ggtaagctgc cctccagcgc cgcatttgcc
     9661 agatgaatca gtgcctctgc ccgtttcaac ggcatgccta acgcttttaa tgcctgcggg
     9721 tcggctgctg ccagccgctg aggcgtgggg aagcagatat actccggaaa atcatccagc
     9781 cgttcgccat aaagctgtac cactttggca gtcaattttg ccgccatcgc cacgctcacc
     9841 agttggccta aaatcgcccg cacgccctgc tcaaaagcat caacacagcc gggtaaacgc
     9901 aatccgggcc gcgccgcgcc taacttgccc aacgcaccgt taacaatctg cgggttacat
     9961 tgcagatcaa gcaggcggct cattttcgcc agacactcag cggcaacggg ttctaaacct
    10021 gcacttaaat ttatgtgcag agtatggcgg gctatatccg gaatagcagt caccacgccg
    10081 cgatattcgc caaccgccag actacgggca taataatcgt cagcgaccgt ttccacaccg
    10141 ttcaccgcac gggcggcgag aaatcccaac atccacaacc agtcatacgg cggctgccag
    10201 ttcagggtat acatcgcatc tccttattca tccgctttca gcataaacgt tattcagacg
    10261 ctgcgctttg ctttcatatt ccggttgtcg cgacggcaac atttcgctaa agtcacgccc
    10321 cttcttcacc ggcatgggga ttatttcgtg tttattggtt ttgattacgg tacagcaaac
    10381 tgttcagtgg cggtcatgcg tgacggtaaa ccgcacttgc taaaaatgga aaacgacagc
    10441 acgctgctgc cttcaatgct ttgcgcgcca acgcgtgaag cggtaagcga atggctgtac
    10501 cgccatcatg atgttccggc agacgacgat gaaacgcagg cgctgttgcg tcgggcgatt
    10561 cgttataacc gcgaagaaga tatcgatgtt acggcgaaaa gcgtgcagtt cggtctttcc
    10621 tcactggcac agtacattga tgatccagaa gaagtgtggt ttgtgaaatc accaaaatcg
    10681 ttcctcggtg ccagcggctt aaaaccgcag caggtagcgc tgtttgagga tctggtctgc
    10741 gcaatgatgt tgcacattcg ccagcaggcg caggcgcagc tgccagaagc gattactcag
    10801 gcggtgatcg gtcgccctat caacttccag gggcttggcg gcgatgaagc aaacacccag
    10861 gcgcagggga ttctggaacg cgcggcgaag cgtgccgggt ttaaggatgt ggtattccag
    10921 tacgagccgg tagcggcagg gctggattac gaagcgactt tgcaggaaga aaaacgggtg
    10981 ctggtggtgg atatcggcgg tggtacgact gactgttcat tgctgctgat ggggccgcag
    11041 tggcgttcgc gtctcgatcg tgaagccagc ctgctgggtc acagtggttg ccgtattggc
    11101 ggtaacgatc tggatatcgc actggcgttt aaaaacctga tgccactgct gggtatgggt
    11161 ggcgaaaccg aaaaaggcat cgccctgccg attctgccgt ggtggaatgc ggttgccatc
    11221 aacgacgttc ctgcgcagag tgatttctac agtagtgcca acggtcgtct gcttaacgat
    11281 ctggtacgcg atgcccgcga accggaaaaa gtggccctgc tacagaaagt ctggcgtcag
    11341 cgtttaagct atcgcctggt gcgtagcgca gaagagtgca aaattgctct ttcaagcgta
    11401 gcggaaatcc gcgcctcact gccgtttatc agcgatgaac tggctacgct gattagccag
    11461 caagggctgg aaagcgccct cagccagcca ctggcgcgga ttctggaaca ggtgcaactg
    11521 gccctcgata acgcccagga aaaaccggat gtcatctacc tgaccggcgg tagcgcccgt
    11581 tcgccgctga ttaaaaaagc gctggcagaa cagttgccgg gcattccgat tgcaggcggc
    11641 gatgactttg gctccgtcac cgccgggctg gcacgctggg cggaagtggt gtttcgttaa
    11701 ttgaaaaatg gctcagcagc ggcttaaatc tgcctgcgcc tgagccagtt tttctgccgc
    11761 ctgacgtaac ggttccatca acggcatggt ccgtgctgga gcatgaaggc ggaatcgctg
    11821 caactctgtc gcgccgacag tcaacgccga ttccagccga tgtcgtttgg cggccatttt
    11881 cgccattacc gcttgtcttt ctgcaggcgt taccgcttcg ttcggcctga acacaaaacg
    11941 gcgttcacag ctcgctttcc agtcgatgac cgcctgggtc agatgatcac caaacccttt
    12001 cacttgctta accccacgac gggtaacatc cgcggctgtt tcaataccaa aagaccgtaa
    12061 cgccgcttta cgcgcagggc caacgccggg aatagaggca gcatcaataa aaaatccctc
    12121 cagaaacttc tgcttctgcc gttcccttgc agtgtcgtga agtgctgcca gagcacgttt
    12181 ttcttcttcc ggtaacccga gaatttcgtc cttcattttt tcgagcatcg tccgtttggc
    12241 gataaaacct tccagcccgc ccaactgttg gatctggctg actaaatggt catagtccat
    12301 tttcgcgcga ttaaatgcct cacggcgttg ctggatttct gctttataag ctttgcttgt
    12361 cagactgcca ataatccaga tagccgccag cacaggaacc aaaataatat aacgcgattc
    12421 tgcctgaagg ccgcaaagca acagcgataa cgctgacagt gcgatctcaa gcagaatgat
    12481 gtattcgcgc cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga aatgatcggg
    12541 taatggcaat tgcactgctg gcggtgctac tgacgccatc accatcgccc agactttcgc
    12601 cagcacaaaa ttcccgccgg tggtaatgac ctcttcgccg agatcaataa aatagataac
    12661 gccttgatta tccagcgtac accacgggca gtcagtcaaa tgagcgggat aaacatgcat
    12721 tgccgaaacg gtacatttct ttaactgttg gcgtagagag tccagtgccg ctacccacgc
    12781 ttttgccgtc ggacgcgcgg ttgccacgcc actttccgtg aatgcctgct gaaacatggc
    12841 ttcaacatcg cccggtaaca tcgacagcgg aatcgatcgt ggcggcggtt ttaaaccacg
    12901 tcgctggttg tctgacgcgt aggcataacg gaaatgggca atatccgtct ccagcgcatt
    12961 acccgcatca gagataagcg gcacaccaga ataaggatgc cgcccaccaa acaagacgtg
    13021 aaaaatcagc aacgcaaggc caaaattatc gtgattcgcg gtacgttcaa aaccgacaaa
    13081 tgatggcaag gtttgtagct ctggcggcgt aaaatgcgac acgccgactt cgcataaatg
    13141 cagtgtgcca ttggcgttaa tctgaaagga gtcactatcg atcaacacca ctttgctgtc
    13201 gcgacctacc ataaagctgt tctggtttac gtcccccacg acgtgcccgt gctcgtgaac
    13261 cgtagcaaaa gatgaagcaa tattgcgcgc aacatagagt agaaaatccc acgcacaatg
    13321 agggtaactc tggcgacgat gtgccgggct atagatcata tgaatcggtt ctttaccaga
    13381 aacttttggc atcataaaac cgataacttt tccgcctcgc ccaccgtgaa gcgttgcctg
    13441 cggccaggcg acataattca acaactgcgc gtcagctgtc gcagccataa aggcaagttt
    13501 gtcctgtttt aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat cggcaaactc
    13561 ctcgatatca taaaccgcac cttcaccgcc tttgcccagt tcacggcctg gcgtaacgca
    13621 ctcaccagta gcagtatata aagtgggttt catggtaacc acagcgccag tgccaacgtt
    13681 ttatcatcat cggtacgctc gtttaccgcc gggctcgaca aaaactgctt taacaattca
    13741 ggtaataaat cgagttgttc ctgcgttgct gccgccagtc cattgaaaaa cggggtaaag
    13801 aaaggcacat gcggagaatt atccagcata tttaacgcca gtcgctgaat accatccgta
    13861 aatgctgcaa ctttatgcgc acgctcagtg ctggtgaaag tttccagtct ggaaacagca
    13921 tcttcatcgc taatgaaatg cgtcatattg gcatattcac ccaccatcgg cgtgagcggc
    13981 agttccagac cacgaccaaa atccaccact acaccgccgt cgccaatttg cataatcaag
    14041 gtgccgttag ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg aactgctaat
    14101 gctttggctt ctgcctcggc aaaaagtcgt tgacgaatgg tcagtaccat atttgtggcg
    14161 aggacatcat taagccccaa ttcaccgtcc tgtacttttt gcaccataaa cgccatcgct
    14221 tcattgacgg cgagcttcgc gccttctcct ccctgcgaaa cgctacctgc gccatcagca
    14281 aggaacatca ccagtaatgg ctgctggtca tttagccagg caacctgcat ctggcaggca
    14341 tcctgacaag gtaaatcagc gctaatgtgc gacgttccaa cagcggatgc ataaaccaga
    14401 cgccagctca cactgacgtc cagccttttg gcgcttccag cacaacttcc gttcccggcg
    14461 tggagcggga aaccgaacgc agcgaactgg ataaccagct aaacagttca cggaattgta
    14521 atccctgcag tggcaaaggc tgacgaacgc tgatttgtgc cagagtcttc atatccgccc
    14581 cctgaacacc gatggaaaag aaggcaaact tcttatcttc ttccccctga aacactttgt
    14641 tggcggcagc ctgccactca tcggttggag cgccatcggt gatcaggaaa atccacgggc
    14701 gataatacga aataccattt gcgcggtact cacgtttcct ctcttcaacc atatccaggg
    14761 ctttggtaat ggcggccccc ataggcgtat ccccctgggc aaacaggatg ggcgggaaaa
    14821 agttagcggc gctggtaaag ggttgttcca catgcaccgg gccgaacgtc acaatgccga
    14881 gttccactct ttttaaagcc agcggatcag caagcagttc atcacgaaag gtaaccaatc
    14941 cggcgttaag ttcattaata ggtctgccgc tcatcgagcc agaaacatcc agcagcagaa
    15001 tgcaggggca acgtggttcc gggttactgg caaaatcgct ggtggcaaac gtgatttgtt
    15061 cgctcataag aaatcctttc ttagagaatt attgaggtcc ggtatcatac cgattgcaat
    15121 aatgaaataa gagcaaacga agaaactttc agaattaggc ccggcggcgc agaaaatttt
    15181 gctattcgcc tcgcaagaat ggagagccgt cgattgacac taagggcgga gtgacataat
    15241 ttcaggagtg agggttaggg agaggtttcc ctctccccct agtgttctta gtaagcctgg
    15301 aagctaatca ctaagagtat caccagtatg atgacgtgct tcatcataac cctttcctta
    15361 tttaaggccc cttcctcggg aggggctttc tcgtttcagc atcccgctga aatcgtcggc
    15421 ttacctcctt tcgccctgaa tacagtctat cgctaacgcg tgaacaaaat agtttcctct
    15481 gtgattattg cgtaacgact cgcaaagagt atatttgctg gcaggatcgc agactgcaaa
    15541 gcctgcggat tgacaatctg accgtgaagg catactttca ggagtgaggg ttgagcgggg
    15601 tttcccccgc cctggtagtc ttagtaagcc gggaagctta tgactaagag caccacgatg
    15661 atgatccact tcatcatgac cctttcctta tttatggccc cttcctcggg aggggctttc
    15721 ccgtttcagc gtcccgctga aatcgtcggc ttacctcctt tcgccctgaa ttcagtctat
    15781 cgctaacgcg tgaacaaaat agtttcctct gtgattattg cgtaacgtct cgcaaagaat
    15841 atttgctggc aggatcgcag actgcaaagc ctgcggattg acattctgaa cgtgaaggca
    15901 tactttcagg agtgagggta gagcggggtt tcccccgccc tggtagtctt agtatgctgg
    15961 gtagctaatc actaagagca ccacgatgat gagtatcttc atcatgaccc tttccttatt
    16021 tatggcccct tcctcgggag gggctttccc gtttcagcgt cccgctgaaa tcgtcggctt
    16081 acctcctttc gccatgcaag cagtctatcg ctaacgcgta gataaaatag tttcctgtgt
    16141 tattactgga tgcgtgctcg caaatgtgcc cgccattcag acgattcccg atagtgtttc
    16201 ataattcctc catttttctc ccttattggc tggctacact agtatcattc cgcgaaacgt
    16261 ttcaggaaga gaaactctta acgatgaaag gcagttataa atcccgttgg gtaatcgtaa
    16321 tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc aatgactccc
    16381 agagtgcagc cccaggggcg acgaaacaag cgcagcaatc gccagcgggt ggtcgccgtg
    16441 gtatgcgttc cggcccatta gccccggttc aggcggcgac cgccgtagaa caggcagttc
    16501 cgcgttacct caccgggctt ggcaccatta ccgctgctaa caccgttacg gtgcgcagcc
    16561 gcgtagatgg tcagttgatg gcgttacatt tccaggaagg ccagcaggtc aaagcaggcg
    16621 atttactggc agaaattgac cccagccagt tcaaagttgc attagcacaa gcccagggcc
    16681 aactggcaaa agataaagcc acgcttgcta acgcccgccg tgatctggcg cgttatcagc
    16741 aactggcaaa aaccaatctc gtctcccgtc aggaactgga tgcccaacaa gcgctggtca
    16801 gtgaaaccga aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg cagctgcaac
    16861 tcgactggag ccgcatcacc gcaccagtcg atggtcgcgt tggtctcaag caggttgatg
    16921 ttggtaacca aatctccagt ggtgatacca ccgggatcgt ggtgatcacc cagacgcatc
    16981 ctatcgattt gctctttacc ctgccggaaa gcgatatcgc taccgtagtg caggcgcaaa
    17041 aagccggaaa accgctggtg gtagaagcct gggatcgcac caactcgaag aaattaagtg
    17101 aaggcacgct gttaagtctc gataaccaaa tcgatgccac taccggtacg attaaagtga
    17161 aagcacgctt taataatcag gatgatgcgc tgtttcccaa tcagtttgtt aacgcgcgca
    17221 tgttagtcga caccgaacaa aacgccgtag tgatcccaac agccgccctg caaatgggca
    17281 atgaaggcca ttttgtctgg gtgctgaata gcgaaaacaa ggtcagcaaa catctggtga
    17341 cgccgggcat tcaggacagt cagaaagtgg tgatccgtgc aggtatttca gcgggcgatc
    17401 gcgtggtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa gtggtggaag
    17461 cccagagcgc caccactccg gaagagaaag ccaccagccg cgaatacgcg aaaaaaggag
    17521 cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc gcctgtttat
    17581 tatgcgtcct gtcgccacca cgctgctgat ggtggcgatc ttactcgccg ggattatcgg
    17641 ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca ttcaggtggt
    17701 cacgctctac ccaggtgcga gcccggatgt catgacctct gccgttaccg cgcctctcga
    17761 acgccagttc gggcagatgt ctggtctgaa acagatgtcg tcgcaaagtt ccggcggtgc
    17821 ttcagttatc actttgcagt tccagctaac attaccgctg gatgtcgccg agcaggaagt
    17881 acaggccgcg attaacgctg cgaccaactt gttgccgagc gatctgccta acccgccggt
    17941 ttacagcaaa gtgaacccgg cagatccgcc gatcatgacg ctcgccgtca cctcaacagc
    18001 catgccgatg acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga aaatctcgca
    18061 gatttccggc gtcggcctgg tgacgctttc cggcggacag cgtccggctg ttcgcgtcaa
    18121 acttaacgct caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc gaaccgccat
    18181 taccggcgct aacgttaact cggcaaaagg tagccttgac ggcccttccc gcgcggttac
    18241 gctttccgcg aatgaccaaa tgcaatctgc ggaagaatat cgccagttga ttatcgccta
    18301 ccagaacggc gcgccgattc gtctgggcga tgtcgcaact gtagagcaag gtgcagaaaa
    18361 cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg ttcagcgcca
    18421 gcccggtgct aacattatct ccaccgctga cagcatccgg cagatgctgc cacagctcac
    18481 cgagagtttg ccgaaatcgg tgaaggtgac ggtactttct gaccgtacca ccaatatccg
    18541 cgcatccgtc gatgatactc agtttgaatt gatgatggct atcgcgctgg tagtcatgat
    18601 tatctacctg tttttgcgca atattccggc gaccatcatt cccggcgtcg cagtgccgct
    18661 gtcgttaatc ggcaccttcg cggtcatggt gtttctcgat ttttcaatca ataacctgac
    18721 gctgatggcg ttaactatcg ccaccgggtt cgtggtcgat gacgccatcg tggtgatcga
    18781 aaatatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc tcaaaggcgc
    18841 aggtgaaatc ggctttacca ttatctcgct gaccttctcg ctgattgcgg tgttgatccc
    18901 actgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta ttaccctggc
    18961 ggtagcgatt ttaatctcag ccgtggtgtc gctaaccctg acaccgatga tgtgcgcacg
    19021 gatgctcagc caggagtcgt tgcgtaaaca gaaccgcttc tcccgtgcct cggaaaaaat
    19081 gttcgacagg ataatcgccg cctatggtcg tggactggcg aaagtgctga atcatccgtg
    19141 gctgacctta agcgtggcgc tcagcacgct gctgcttagc gttctattgt gggtgttcat
    19201 tccgaaagtt ttcttcccgg tacaggataa cggcattatt cagggcactt tgcaggcacc
    19261 gcaatccagc tcctttgcca atatggccca gcggcaacgc caggtcgcgg acgtgatttt
    19321 gcaggatccg gcagtgcaaa gtctgacctc atttgttggc gttgatggca ctaacccgtc
    19381 gctgaacagt gcgcgtttgc agatcaacct caaaccgttg gatgaacgtg atgatcgggt
    19441 gcaaaaagtc atcgcccgtc tgcaaacggc ggtggataaa gtgccgggcg tcgatctctt
    19501 cctgcaacca acgcaggacc tgaccatcga tactcaggtc agccgcaccc agtaccagtt
    19561 taccttgcag gccacgtcac tggatgcgct cagtacctgg gtgccacagt tgatggaaaa
    19621 actccagcag ctgccacagc tttctgatgt ctccagcgac tggcaggaca aagggctggt
    19681 ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagta tggcggatgt
    19741 cgataacgcc ctgtacaacg cgtttggtca gcggctgatt tccactattt atactcaggc
    19801 caaccagtat cgcgtggtgc tggagcacaa caccgaaaat accccagggc ttgcggcgct
    19861 ggataccatt cgcctgacca gcagcgatgg cggcgtggta ccgctaagct caattgccaa
    19921 aattgagcag cgttttgcgc cgctttccat caaccatctg gatcagttcc cggtaacgac
    19981 catctctttt aacgtgccag ataactattc gctgggcgat gcggtgcagg cgattatgga
    20041 caccgaaaag acgctgaatc tgccggtgga tatcaccacg cagttccagg gcagcaccct
    20101 cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg tggtggcgat
    20161 gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca ttctctcgac
    20221 gctacccacg gcaggtgttg gcgccctgct ggcgttaatg attgccggta gcgaactgga
    20281 tgtgattgcg ataattggca ttattttgct gatcggtatc gtgaagaaga acgccatcat
    20341 gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc gcgatgcaat
    20401 ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accaccctgg cggctctgct
    20461 tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc gtccgttagg
    20521 tatcggcatg gtcggcggcc tgattgtcag ccaggtgctg acgctgttca ccacgccggt
    20581 gatttatctg ctgtttgacc gcctggcatt gtggaccaaa agtcgctttg ctcgccatga
    20641 agaggaggcg taagtgaagt tttttgccct cttcatttac cgcccggtgg cgacgatttt
    20701 actgtcggtc gccattaccc tatgcggcat actgggtttc cgtatgctgc cggtcgcccc
    20761 gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg gtgcgtcgcc
    20821 ggagacaatg gcgtcttccg ttgccacgcc gctggagcgc tcacttgggc gcattgccgg
    20881 ggtcagtgaa atgacctcca gcagttcgct cggcagcacg cgtattattt tgcagtttga
    20941 ttttgaccgg gatatcaacg gtgcggcgcg tgatgtgcag gcggcgatca acgccgcaca
    21001 aagtctgctg cccagtggga tgcccagccg cccgacttat cgcaaagcga acccgtcgga
    21061 tgcgccaatt atgatcctga cgctaacctc cgatacttat tcgcagggtg aattgtacga
    21121 tttcgcctcg acgcagctgg ctccgacgat ttcgcaaatc gacggcgtcg gtgatgtcga
    21181 tgtcggcggc agctcgctgc ccgccgtgcg cgtcgggctg aacccgcagg cgctgtttaa
    21241 tcagggcgtg tcgctggacg acgtacgcac tgccatcagc aatgccaacg tgcgtaaacc
    21301 gcagggggcg ctggaagatg gcactcaccg ctggcagatc cagaccaatg atgagctaaa
    21361 aaccgccgct gaatatcagc cgttgattat tcattacaac aacggcggcg cggttcgtct
    21421 gggcgatgtg gcgacggtga ccgactcggt gcaggatgtg cgcaacgccg ggatgaccaa
    21481 cgccaaaccg gctattttac tgatgatccg caaactgccg gaagccaata ttatccagac
    21541 ggtagacagc atccgggcaa aattaccgga gttgcaggag accattccgg cggcgattga
    21601 tctgcaaatt gcccaggatc gctcccccac cattcgcgcc tcgctggaag aagtcgagca
    21661 aacgctgatt atctcagtgg cgctggtgat tctggtggtg tttttattcc tgcgctcggg
    21721 tcgcgccact attattcccg ccgttgcggt gccggtttcg ctgattggta cgtttgcggc
    21781 gatgtacttg tgcggattca gtctcaataa cctgtcgtta atggcgctca ccatcgctac
    21841 tggtttcgtg gtggatgacg ccatcgtggt gctggaaaac attgcccgtc atctggaagc
    21901 ggggatgaaa ccactacagg ccgcgctaca aggtactcgc gaagtcggct ttacggtgct
    21961 gtcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg gcggattgcc
    22021 gggccgactg ttacgcgagt ttgccgtgac gctttctgtc gccattggta tttcgttgct
    22081 ggtttctctg acattaacgc caatgatgtg tggctggatg ctgaaagcca gcaagccgcg
    22141 cgagcaaaag cgactgcgtg gttttggtcg catgttggta gccctgcaac aaggctatgg
    22201 taagtcactg aaatgggtgc tcaatcatac ccgtctggtg ggcgtggtgc tgcttggcac
    22261 cattgctttg aatatctggc tctatatctc gatcccgaaa accttcttcc cggagcagga
    22321 caccggcgtg ttgatgggcg ggatacaggc ggatcagagt atttcgtttc aggcgatgcg
    22381 cggcaaattg caggatttca tgaaaattat ccgtgacgat ccggcggtgg ataatgtcac
    22441 cggatttact ggcggttcgc gggtgaatag cgggatgatg tttatcaccc tcaagccacg
    22501 cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgggtga agctggcgaa
    22561 agaaccgggg gcgaatctgt tcctgatggc ggtacaggat attcgcgttg gcggacgtca
    22621 gtcgaacgcc agttatcagt acacgttgtt atccgacgac ctggcggcac tgcgcgaatg
    22681 ggagccgaaa atccgcaaaa aactggcaac gttgccagaa ctggcggacg tgaactccga
    22741 ccagcaggat aacggcgcag aaatgaatct ggtttacgat cgcgacacga tggcacggct
    22801 gggaatcgac gtgcaggccg ccaacagcct gttaaataac gccttcggtc agcggcaaat
    22861 ctcgaccatt taccagccga tgaaccagta taaagtggtg atggaagtgg atccgcgcta
    22921 tacccaggac atcagcgcgc tggaaaaaat gttcgttatc aataacgaag gagaagcgat
    22981 cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc ccactatcgg tgaatcatca
    23041 gggattatcg gcggcctcga ccatttcgtt taacctgccg accgggaaat cgctctcgga
    23101 cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga cggtgcgcgg
    23161 cagttttgcc ggcacggcgc aggtgttcca ggagacgatg aactcacagg tgatcctgat
    23221 tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga gttacgtaca
    23281 tccgctgacg attctctcca ccctgccctc ggcgggcgtt ggagcgctgc tggcgctgga
    23341 gctgttcaat gccccgttca gcctaatcgc cctgataggg atcatgctat taatcggcat
    23401 cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt gaagcccaac ggcacggtaa
    23461 cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc cgattatgat
    23521 gactaccctg gcggcgctgt ttggtgcgct gccgctggta ttgtcgggcg gcgacggatc
    23581 ggagttgcgc caacccctgg ggatcaccat tgtcggcggt ctggtaatga gccagctcct
    23641 gacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctacggc tgcgtttttc
    23701 gcgtaaacct aaacaaacgg taaccgagta aatgacagat cttcccgaca gcacccgctg
    23761 gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca ccatcgttaa
    23821 caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata tgcacatggt
    23881 tattgtctct tatgtgctga ccgtggcggt gatgctgcct gccagcggct ggctggcgga
    23941 caaagtcggc gtgcgcaata ttttctttac cgctatcgtg ctgtttactc tcggttcact
    24001 gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt tacagggcgt
    24061 tggcggtgcg atgatggtgc cggtcggaag gcttacggtg atgaaaatcg taccgcgcga
    24121 gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc cgctgctcgg
    24181 tccggcgctt ggcggcctgc tggtggagta cgcgtcgtgg cactggatct ttttgatcaa
    24241 cattccggtg gggattatcg gtgcgatcgc cacattgatg ttaatgccga actacaccat
    24301 gcagacgcgg cgctttgatc tctccggctt tttattgctg gcggttggca tggcggtatt
    24361 aaccctggcg ctggacggca gtaaaggtac aggtttatcg ccgctggcga ttgctggcct
    24421 ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg cacgccagaa ataaccaccg
    24481 cgctcttttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc tggcggggag
    24541 ctttgccgga cgtatcggta gcggcatgtt gccctttatg acgccggttt tcctgcaaat
    24601 tggcctcggt ttctcgcctt ttcatgccgg actgatgatg atcccgatgg tgcttggcag
    24661 catgggaatg aagcgaattg tggttcaggt ggtgaatcgc tttggttatc gtcgggtact
    24721 ggtagcgacc acgctgggtc tgtcgctggt caccctgttg tttatgacta ccgctctgtt
    24781 gggctggtac tacgttttgc cgttcgtcct gtttttacaa gggatggtca actcgacccg
    24841 tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg cgagcagcgg
    24901 caacagtctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca ccatcgccgg
    24961 gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca ccacacaaac
    25021 cgtctttatg tacacctggc ttagcatggc gtttatcatc gcccttccgg cgttcatctt
    25081 tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa aaaggagcgc
    25141 gcaatgaagt tctggcgacc cggtattacc ggcaaactgt ttctggcgat tttcgccacc
    25201 tgcattgtct tgctgatcag tatgcactgg gcggtgcgta tcagttttga gcgtggcttt
    25261 attgattaca tcaagcatgg taatgaacag cgattacaac tgttaagtga tgcgcttggc
    25321 gagcagtatg cgcagcatgg caactggcgc ttcctgcgca acaatgatcg ctttgtcttt
    25381 cagatcctgc gttcatttga acacgataat tcggaagata aacccggccc gggtatgcca
    25441 ccgcacggct ggcgtaccca gttctgggtg gttgatcaaa acaacaaagt gctggttggt
    25501 ccgcgagcgc cgattccacc tgacggtaca cggcgaccca ttctggtcaa cggtgcggaa
    25561 gttggcgcgg tgatcgcctc ccccgttgag cggttaacgc gcaatactga tatcaatttc
    25621 gataaacaac agcggcaaac cagctggttg attgtcgctc tggcaacgtt actcgcggcg
    25681 ctcgccacct ttctgctggc gcgcggtctt ttggccccgg tcaaacggct ggtggacggc
    25741 acgcacaaac tggcggcggg cgatttcaca acccgcgtga cgcccaccag tgaagatgaa
    25801 ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca ctctagagaa aaaccagcaa
    25861 atgcggcgcg attttatggc cgatatttct cacgaactgc gtacgccatt agcggtgctg
    25921 cgcggtgaac tggaagccat tcaggatggc gtgcgtaaat tcacgccgga gacggtggcg
    25981 tctttacagg cggaggtcgg tacactgacc aaactggttg acgatcttca tcagttgtcg
    26041 atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg tagatttgat cccactgctg
    26101 gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc gtggcctgaa actgcaattt
    26161 tccctgccag acagtattac cgtatttggc gatcgcgacc gtttaatgca gttattcaat
    26221 aacttactgg aaaacagcct gcgctacact gacagcggcg gcagcctgaa aatctctgcc
    26281 gagcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg tgtcagtgac
    26341 gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg aaggttcccg caaccgtgcc
    26401 agcggcggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc acataatggc
    26461 cgcattattg ctgcccattc gccttttggc ggggtaagca ttacagtaga gttaccgctg
    26521 gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac acaccgcgta
    26581 ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat ctgcgtgctg
    26641 cgagctatgc gccgacgctt attagccacg gcgatcaggt actgccgtat gtgcgccaga
    26701 caccaccgga tctgatcctg ttggatctga tgctccctgg caccgatggc ttgacgctgt
    26761 gccgggaaat tcgtcgtttt tccgatgttc ctgtcgtgat ggtgacggca aaaatcgaag
    26821 agatcgaccg cctgctgggg ctggagattg gcgcagatga ctacatctgc aagccgtaca
    26881 gcccacggga agtggtagcg cgcgtcaaaa ccattttgcg ccgttgcaaa ccgcagcgcg
    26941 agttgcagca acaggatgct gaaagcccgt tgattatcga cgaaggtcgt tttcaggctt
    27001 catggcgcgg taaaatgctt gacctgacgc ctgcggaatt tcgtctgctg aaaacgctct
    27061 ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct caatcatctt tatgacgact
    27121 accgcgtagt aaccgaccgc accattgaca gccacattaa aaacctgcgc cgcaagctgg
    27181 aatctctcga tgccgaacag tcatttatcc gcgccgttta cggcgttggt taccgctggg
    27241 aagccgacgc ctgccgcatc gtttagtttt agcgacatta ctttgttagc cggagatggc
    27301 gttcagaccc gatccggcaa cattatccca cgcatggtca gcaaactgac attatctctc
    27361 cgccctggca ggcatcagaa aatcgctcat actttaatcg gtaaactgca cctttagatg
    27421 ctgttttcga tacgcaattt caatcaagga gtcattatgg ctggttggtt tgaactcagt
    27481 aagagcagtg atagtcagtt ccggtttgtg ctaaaagcgg gcaatggtga gacgatcctc
    27541 accagcgagc tgtatacctc aaaagcctct gcggaaaagg gtatcgcgtc ggtgcgtagc
    27601 aacagcccgc aagaagaacg ctatgagaaa aaaacggcaa gtaacggcaa attctatttc
    27661 aatctgaaag ccgccaatca tcaaattatc ggctccagcc agatgtacgc caccgcgcaa
    27721 tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca caagtcagac ggtgaaagac
    27781 aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc ggctttattt ccaaataacg
    27841 cttatttatt tacctgcacc attaaaatag tggacactga tgaatactca ttgtgctttc
    27901 tttgtttaac atctccatgc ttatcctcct gaacatgttc atctgccaaa atatcgtcta
    27961 atacttttcg tgacgctatc cggtttatga catacactga tgtaagattt ttacttacag
    28021 gagagcataa acatggccag aacgatgacg gttgatctcg gcgatgagtt gcgtgaattt
    28081 atcgagtcct tgattgaatc cggcgactat cgcacccaaa gcgaagtgat ccgtgagtcg
    28141 ctgcgcttac ttcgagaaaa gcaagcagag tctcgcctgc aagcgttacg tgacatgctc
    28201 gcagaagggt tgagtagtgg tgaagctcag ccgtgggaaa aggacgcgtt tttacggaag
    28261 gtgaaagcag ggataaggaa ataatgtgcg tattattaaa ctcatgccaa aagccaatga
    28321 ggatttagaa ggcatctggt attacagcta tcaccatttc ggcgaaccac aagccgacag
    28381 atatgtggaa catctttcag acgttctcca gattctgagt aacaataata taggtacgcc
    28441 cagacctgaa ttaggcgaag gtatttttgt tcttcctttt gaacgccacg taatttattt
    28501 cctgcaatca ccaggggaaa ttatcgtcat tcgtaccctt aaccaaaacc aggacgccac
    28561 cagacattta cactggagct aaacccgccg attgcgctac aatgcccgcc cttaaagtgg
    28621 gggcactccc ctaaccgctt catcaggtga agcggatctg acctgtcatc agaacgagag
    28681 aattatgttt aaaccggaac tcctttcccc ggcgggaacg ctgaaaaata tgcgttacgc
    28741 tttcgcttat ggcgcagatg ctgtttatgc gggccagccg cgttactccc tgcgtgtgcg
    28801 caacaacgaa ttcaaccacg aaaatcttca gctcggcatc aatgaagccc acgcgctggg
    28861 gaaaaagttt tatgtcgtgg tcaacattgc accgcacaac gctaagctga aaacctttat
    28921 ccgtgacctg aaaccggtgg tggaaatggg gccggatgcg ctgattatgt ccgatccagg
    28981 gctgattatg ctggtgcgtg agcacttccc tgaaatgccg atccacctct cggtgcaggc
    29041 taacgccgta aactgggcga cggtgaaatt ctggcagcaa atgggcctga cccgcgtgat
    29101 cctctctcgc gagctgtcgc tggaagagat tgaagagatc cgtaatcagg tgccggatat
    29161 ggagatcgag atcttcgttc acggcgcact gtgcatggcc tactccggtc gctgcctgct
    29221 ctctggctat atcaacaagc gcgacccgaa ccagggtacc tgcaccaacg cctgccgctg
    29281 ggagtacaac gtccaggaag ggaaagaaga tgacgttggc aacatcgtac acaagtacga
    29341 gccgattccg gtgcaaaatg ttgagccgac gctgggtatc ggcgcgccaa ccgacaaagt
    29401 gtttatgatc gaagaagccc agcgtccggg cgagtatatg accgcgtttg aagatgagca
    29461 cggcacttac atcatgaact cgaaagatct gcgcgccatc gcccatgtag aacgcctgac
    29521 caaaatgggc gtgcattcgc tgaaaatcga aggccgtact aaatctttct actattgcgc
    29581 acgcaccgca caggtttatc gtaaagctat cgatgatgcc gctgcgggca aaccgttcga
    29641 taccagcctg ctggaaactc tggaaggtct ggcgcatcgt ggctataccg aaggtttcct
    29701 gcgtcgtcat actcacgacg attatcagaa ctacgaatac ggttactcgg tttctgaccg
    29761 ccagcagttt gttggtgagt ttaccggtga gcgcaagggg gacctcgcgg cggtagcggt
    29821 gaaaaataaa ttctccgttg gcgacagcct tgagctgatg acgccgcaag gcaacattaa
    29881 ctttaccctt gagcacatgg aaaacgccaa aggcgaagct atgccggtag caccaggcga
    29941 tggttatact gtgtggctcc cggtgccgca ggatcttgag ctaaattacg cgctgctgat
    30001 gcgtaatttc tccggggaaa ccacgcgtaa cccacacggt aagtgattaa tttcgattat
    30061 ttttcccgga tggaaaattc ttagaaaccg atcacataca gctgcattta ttaaggttat
    30121 catccgtttc gctgaaaaac ataacccata aaatgctagc tgtaccagga accacctcct
    30181 tagcctgtgt aatctccctt acacgggctt attttttatg cataagccct atccctggtc
    30241 accgtcttcc attgaccaca tcgatagaat ctcccttcat agcacgatgc ctttcactta
    30301 ttggcatcgt gctcgcacag gttccggcta agcacaacca gaacgcgcat gtttgacgct
    30361 taccaaaaaa tattctcact ctccacattt gaatgtcaga cgagcgacgc catgtaatcc
    30421 tgcaccttct gtcttcaggt caactatctg catttttttg ccctgagtaa cacagaaatg
    30481 ggctgcatca ttttttacta tattttctgc accagatatt ctgcccctgg ctaaagaagc
    30541 ttcggcttcg gtgtagtatt ggttatcgag tttacgctga atattacttt tatatgcaag
    30601 accaaattta ccgatacttg tctcatcatt atgcacagca caaccagaca taataaaaat
    30661 actaattaat gatatagcag ctatcttttt catctcacct ttcccc
//
LOCUS       DAZUCE010000033        29637 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZUCE010000033 DAZUCE010000000
VERSION     DAZUCE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.033"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1227)
                     /gene="espL1"
                     /locus_tag="ACHY03_001304"
     CDS             complement(<1..1227)
                     /gene="espL1"
                     /locus_tag="ACHY03_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFS3904560.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPIHAENILITLHYKKEII
                     SLLLETTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYALDSLGNGEGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSAYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFRQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMN"
     gene            1750..3678
                     /gene="thrS"
                     /locus_tag="ACHY03_001305"
     CDS             1750..3678
                     /gene="thrS"
                     /locus_tag="ACHY03_001305"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFS3904561.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            3682..4224
                     /gene="infC"
                     /locus_tag="ACHY03_001306"
     CDS             3682..4224
                     /gene="infC"
                     /locus_tag="ACHY03_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFS3904562.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4321..4518
                     /gene="rpmI"
                     /locus_tag="ACHY03_001307"
     CDS             4321..4518
                     /gene="rpmI"
                     /locus_tag="ACHY03_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFS3904563.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            4571..4927
                     /gene="rplT"
                     /locus_tag="ACHY03_001308"
     CDS             4571..4927
                     /gene="rplT"
                     /locus_tag="ACHY03_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFS3904564.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5044..5168
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5050..5094
                     /gene="pheM"
                     /locus_tag="ACHY03_001309"
     CDS             5050..5094
                     /gene="pheM"
                     /locus_tag="ACHY03_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFS3904565.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5377..6360
                     /gene="pheS"
                     /locus_tag="ACHY03_001310"
     CDS             5377..6360
                     /gene="pheS"
                     /locus_tag="ACHY03_001310"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFS3904566.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6375..8762
                     /gene="pheT"
                     /locus_tag="ACHY03_001311"
     CDS             6375..8762
                     /gene="pheT"
                     /locus_tag="ACHY03_001311"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFS3904567.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8767..9066
                     /gene="ihfA"
                     /locus_tag="ACHY03_001312"
     CDS             8767..9066
                     /gene="ihfA"
                     /locus_tag="ACHY03_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFS3904568.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9167..10147
                     /gene="btuC"
                     /locus_tag="ACHY03_001313"
     CDS             9167..10147
                     /gene="btuC"
                     /locus_tag="ACHY03_001313"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFS3904569.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10210..10761
                     /gene="btuE"
                     /locus_tag="ACHY03_001314"
     CDS             10210..10761
                     /gene="btuE"
                     /locus_tag="ACHY03_001314"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFS3904570.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            10761..11510
                     /gene="btuD"
                     /locus_tag="ACHY03_001315"
     CDS             10761..11510
                     /gene="btuD"
                     /locus_tag="ACHY03_001315"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFS3904571.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            11588..12052
                     /gene="nlpC"
                     /locus_tag="ACHY03_001316"
     CDS             11588..12052
                     /gene="nlpC"
                     /locus_tag="ACHY03_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS3904572.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12299..13012
                     /gene="ydiV"
                     /locus_tag="ACHY03_001317"
     CDS             12299..13012
                     /gene="ydiV"
                     /locus_tag="ACHY03_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFS3904573.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13075..14511
                     /gene="selO"
                     /locus_tag="ACHY03_001318"
     CDS             13075..14511
                     /gene="selO"
                     /locus_tag="ACHY03_001318"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFS3904574.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGPGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(14515..14706)
                     /gene="hemP"
                     /locus_tag="ACHY03_001319"
     CDS             complement(14515..14706)
                     /gene="hemP"
                     /locus_tag="ACHY03_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFS3904575.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQTIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(14838..15884)
                     /gene="aroH"
                     /locus_tag="ACHY03_001320"
     CDS             complement(14838..15884)
                     /gene="aroH"
                     /locus_tag="ACHY03_001320"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFS3904576.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16041..16874)
                     /gene="ppsR"
                     /locus_tag="ACHY03_001321"
     CDS             complement(16041..16874)
                     /gene="ppsR"
                     /locus_tag="ACHY03_001321"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFS3904577.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17207..19585
                     /gene="ppsA"
                     /locus_tag="ACHY03_001322"
     CDS             17207..19585
                     /gene="ppsA"
                     /locus_tag="ACHY03_001322"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFS3904578.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            20075..21475
                     /locus_tag="ACHY03_001323"
     CDS             20075..21475
                     /locus_tag="ACHY03_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFS3904579.1"
                     /translation="MSCYLKVLICAIVALAIWFSPVPDGLTPLTWHILAIFITTVVAF
                     ILQPLPVGAIALIAISFIMLTGMMKTSEALKGFSSTTVWLIVAAFLYAKGFIKTGLGR
                     RIAYLLIRGFGGSSLRLGYTLALSDMIIAPATPSNTARAGGILFPIVRSVSNSFGSEP
                     DQGPRKIGAYLMQTVFHSNCLSSSMFMTASAPNALIVSLAASTLFVDISWGMWTLSAL
                     VPGIIAFITMPLVIYKLYPPEIKKTPEAKALAQEELIKMGPVTRDERVTIGIFLLSLL
                     AWSTSKWTGLDATAVALSGVCLMLMTRIITWQDVQSEKGAWDILVWLGVMICMADKLN
                     QLGLFKWFAVTTSALFTGIPWEITLTVLLIVYCYSHYFFAGSTPHVVAMYAAFGSVSV
                     AAGAPPMMAALSLAFVTNLMSGISHYGNGPAVIYYGAGYVSQREWWRLGFIVMLLNIV
                     IWFGLGAVWWKILGLW"
     gene            21511..22470
                     /locus_tag="ACHY03_001324"
     CDS             21511..22470
                     /locus_tag="ACHY03_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFS3904580.1"
                     /translation="MNLKDIQVAIVGKLSDRLMAKVESTFTAYRLWELTDADAFYTQI
                     APDIQALVTSGNPVMGASRALIEKFPNLKIIASNGVGYDPIDIVAAREHGVIVTNTPG
                     VLNDCVADIGMALLLNVARRINIADRYVRDGRWPNEGRFPMATKVSGKRCGIVGLGNI
                     GNAVARRAAAFEMEIHYFDPKPHNKPGWTAHDSLVSLAQAVDFLVLTLPGGASTRGVI
                     DKTVLQALGGTGHLISISRGSVVNETDLIDALQNNIIAGAALDVYANEPHVPEQLMSL
                     DNVVLTPHIASGTSETFNAMADLVFDNLQAFFSGQPVITPVTQ"
     gene            22481..22960
                     /locus_tag="ACHY03_001325"
     CDS             22481..22960
                     /locus_tag="ACHY03_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFS3904581.1"
                     /translation="MIYGDITYLSEQQKRTFHPVIARVIEYLSQHDLNSVPVGEKVFI
                     QDDSIFYTVVEPMTKPLHECRPEVHRKYLDVHFLIAGEERICVDFDDGENSVVESHLG
                     DRDVIFYADERINDRIITLTPGQFVVVFPEEVHRACYKVDNVEKIKKTVVKIDMSLL"
     gene            22973..23716
                     /locus_tag="ACHY03_001326"
     CDS             22973..23716
                     /locus_tag="ACHY03_001326"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3904582.1"
                     /translation="MTKIALVTGGSKGIGYAAARALYDIGYTVIVAARNEETLQQCAQ
                     ELAKERFIPLVADVTDESAVVALFAEIKARFGRLDLLFNNAGNNSPAKSIEEVEFTEW
                     IRVFNANIHSTFLCSKEAIKIMKAQQPMGGRIINNASISALTPRLYTAPYTASKHAVT
                     GLTKAISLDCRKFNIACGQINIGNAETTLSNRMRKGVYQADGAIKAEDMMNVADVAAA
                     IQMMAQLPVTTNVLEITIMANNMPYVGRG"
     gene            complement(23767..25407)
                     /gene="fadK"
                     /locus_tag="ACHY03_001327"
     CDS             complement(23767..25407)
                     /gene="fadK"
                     /locus_tag="ACHY03_001327"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFS3904583.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALNHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRIMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIVQRLEQSCVEA"
     gene            complement(25464..25757)
                     /gene="ydiT"
                     /locus_tag="ACHY03_001328"
     CDS             complement(25464..25757)
                     /gene="ydiT"
                     /locus_tag="ACHY03_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFS3904584.1"
                     /translation="MSQNTTVNVDIKLGVNKFHVDEGHPHIILAANPDINEFQKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(25754..27043)
                     /gene="ydiS"
                     /locus_tag="ACHY03_001329"
     CDS             complement(25754..27043)
                     /gene="ydiS"
                     /locus_tag="ACHY03_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS3904585.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDTLVREGNKVTGVQAGDDILEANVVILAN
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(27099..28037)
                     /gene="ydiR"
                     /locus_tag="ACHY03_001330"
     CDS             complement(27099..28037)
                     /gene="ydiR"
                     /locus_tag="ACHY03_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFS3904586.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(28057..28821)
                     /gene="ydiQ"
                     /locus_tag="ACHY03_001331"
     CDS             complement(28057..28821)
                     /gene="ydiQ"
                     /locus_tag="ACHY03_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFS3904587.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            29137..>29637
                     /locus_tag="ACHY03_001332"
     CDS             29137..>29637
                     /locus_tag="ACHY03_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFS3904588.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENHLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKN"
BASE COUNT         7365 a         7009 c         7754 g         7509 t
ORIGIN      
        1 gttcatcata gccaaaaata aagcaggtgt accaacgcta ttctgcgcag ttattatttc
       61 aatgatttct ttttcaggga gtatgggcaa ctgtctgaat aatgtaataa gctgttgagt
      121 ctggcctgtg gccattatta acaacattgc cgatggatgt ataggggcaa atggcattcg
      181 tgtcgcgata cccgtgaaaa tagcccctgg aactccctca ggtaataggg gcagaatagc
      241 aattgcacag ttgcgaacat acctatcagc ccaggtctga gcatcgggac ctgaactgat
      301 gaacttatca agcgagagaa agccccgtgc tgtcctgtgg gtgcctctga cggcaacgtt
      361 ggtatcgttc gggtcgtaaa cggacacaac ataatttaat tgcccatacg ccgactgctt
      421 tattcgtagc cttactgaca aagtatgatt gtctatatat aaaatcgcag acatatgact
      481 gacattgttt gccatcattc gacagaagaa aatatctaaa gcgaagccaa aatccttact
      541 cgcgataacg cgactccctg caggaggatg gcgaagtaat gctctgaact ccgcgtcccg
      601 gactaagggg acatgctgct gtatggattc aacggaggag taatgtttaa tcaggttgac
      661 cttgccttct ccatttccta gtgaatcaag ggcgtattgc aatgacagat ggcgacaaaa
      721 aatagcgtca gaaggagatt gcgaaaaaaa tgcactacca ttgagattta ctgttggttg
      781 tatagttttt tctctatgga tttttttaga cagtttcata caatggttac gattttcggc
      841 agttaactgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
      901 cgtggtttcc agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      961 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
     1021 ttgtgatatt tttcgacagt ttgcggtaaa ttcctgagta acacagtatg acagcatcga
     1081 acttaaatca gaggtatcaa gtagggtgat gagttggcgc gcaaattgat taataggctc
     1141 tgatgaaggc gttggtatgt cgctatcgat gtgcattcta tgaggagact tataatgagt
     1201 tctgataata atgtcgtttt gactcataat ctattcgcct tatatatgtc tggaggtttt
     1261 gaggatgatg attaattatt atcgccagaa cgaaatgtgc acgaaaaatt gctataagaa
     1321 ttgtgtcact tttatcattg tgatatgacc agttcgtcat taatctcatg atcgtttaaa
     1381 tagtaccgcc aaatcatgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
     1441 ctcgcagtaa acctttcaga cgctccgtga tgttatcagt tgttctttaa gcgttttgct
     1501 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
     1561 cccttttttt agtgttaaca tcgctcaacc gggtggaagg tgagaggtta atgtctttca
     1621 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
     1681 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
     1741 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
     1801 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1861 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1921 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1981 ctgttagggc acgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     2041 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     2101 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     2161 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtc
     2221 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     2281 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     2341 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     2401 atttacggta cggcgtgggc agacaaaaaa gcgcttaacg cttacctgca gcgcctggaa
     2461 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     2521 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     2581 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     2641 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     2701 ttcaccacgt cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     2761 gtacaaattt tcaaccaggg gctgaagtcg tatcgcgatc tgccgctgcg tatggccgag
     2821 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2881 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2941 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     3001 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     3061 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     3121 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     3181 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     3241 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tcttgggtcg
     3301 atggaacgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     3361 gcgccggttc aggttgttat catgaatatt accgattcac agtctgatta cgttaacgaa
     3421 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     3481 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     3541 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     3601 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     3661 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     3721 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     3781 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3841 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3901 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3961 aaggaaatta aattccgtcc tggtacagat gaaggcgact atcaggtaaa actccgcagc
     4021 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     4081 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     4141 gaactggcag tggtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     4201 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tgtctgtgga gccttcgggc
     4261 ttcacaggct ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     4321 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     4381 ggttttaagc acaagcacgc taacctgcgt cacattctga ccaaaaaagc gaccaaacgt
     4441 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     4501 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     4561 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     4621 tttgaaacaa gctaaaggct actacggtgc acgttctcgc gtataccgcg ttgccttcca
     4681 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     4741 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     4801 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4861 tatcgcagta ttcgacaaag tagcgttcac cgctctggtt gaaaaagcga aagcagctct
     4921 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4981 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     5041 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     5101 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     5161 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     5221 cagtctggat acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     5281 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     5341 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     5401 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga caatgtgcgc
     5461 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     5521 ccgccagaag agcgtccggc agccggtgcg gttatcaacg aagcgaaaga gcaggttcag
     5581 caggcgctga atgcacgtaa agcggaactg gaaagcgcag cgctgaatgc gcgtctggcg
     5641 gcggaaacga ttgatgtctc cctgccgggt cgtcgcattg aaaacggcgg tctgcatccg
     5701 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     5761 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     5821 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5881 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5941 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     6001 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     6061 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     6121 ccgtttaccg aaccttctgc agaagtggat gtcatgggta aaaacggtaa atggctggaa
     6181 gtactgggct gcgggatggt gcatccgaac gtgctgcgta acgtcggcat cgacccggaa
     6241 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     6301 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     6361 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     6421 ttgatagcga tgcgctggcg aatcaaatca cgatggcggg cctggaagtt gacggtgtag
     6481 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     6541 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     6601 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gcgaccattg
     6661 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgcggc gaaccgtctg
     6721 aagggatgct gtgctccttt tctgagctgg gaatttctga cgaccataac ggcattatcg
     6781 aactgcctgc agatgcgccg attggcactg acatccgcga atacctgaaa ctcgatgaca
     6841 acaccatcga aatcagcgta acgccaaacc gtgcagactg tttaggtatc attggtgttg
     6901 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6961 gtgcgaccat cgacgacacg ctgccgatta cagtcgaagc gccggaagcc tgcccgcgtt
     7021 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     7081 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     7141 tgctgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     7201 ttgtggtgcg gatggcgaaa gagggcgaaa cgctggtgct gctcgacggt actgaagcga
     7261 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     7321 tcttcggtgg cgaacactct ggcgtgaatg acgaaacaca aaacgtgctg ctggaatgcg
     7381 ctttctttag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg cataccgatg
     7441 cgtctcaccg ttatgagcgt ggcgttgatc cggcactgca gcacaaagcg atggaacgtg
     7501 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     7561 acgaagcaac gctgccgaag cgtgcaacca tcactttacg tcgtagcaaa ctggatcgcc
     7621 tgatcggcca tcatattgcg gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     7681 aagtgaccga aggcaaagac gagtggcagg cagttgcgcc gagctggcgt ttcgacatgg
     7741 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     7801 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7861 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tatagcttcg
     7921 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctgccaagcc
     7981 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     8041 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     8101 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     8161 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     8221 atttgaaagg cgatcttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     8281 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     8341 gtgaacgtat tggttttgtt ggggttgttc atcctgaact ggaacgtaaa ctggatctta
     8401 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     8461 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     8521 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     8581 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     8641 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     8701 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     8761 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     8821 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8881 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8941 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     9001 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     9061 gagtaatctg atctaactaa aaaggccgct ctgcggcctt ttttcttttc actgtcgaag
     9121 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     9181 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     9241 ggcgcttctc ttaagccttt gcgccggtga acaatggatc tcgccaggtg actggtttac
     9301 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     9361 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     9421 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     9481 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     9541 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     9601 attgctggct ggcgttgcat tagggattat ctgtagcgca ctaatgacgt gggctatcta
     9661 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     9721 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     9781 ttgtcagtcc aggccgatga atatgttagc acttggcgag atctcggcgc ggcaactggg
     9841 tttacccctg tggttctggc gcaatgtgct ggtagcagcg accggctgga tggttggcgt
     9901 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9961 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
    10021 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
    10081 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
    10141 acgttagcca caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccca
    10201 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
    10261 accacgctgg agaagtacgc cggtaatgtg ctgttgattg tcaatgtcgc ctcaaagtgt
    10321 ggcttaacgc cgcaatatga gcagttggag aatattcaga aagcctgggc cgatcgaggt
    10381 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
    10441 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
    10501 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgact
    10561 gcagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg tcgtgcaccg
    10621 ctgtaccctg atgatatttt atggaatttt gagaaatttt tagttggcag ggacggattg
    10681 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
    10741 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcaa
    10801 cccgcctggg gccgctttct ggcgaggttc gggctgggga gatcctgcat ctggtggggc
    10861 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10921 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10981 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    11041 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    11101 tggctcttga tgacaaactc ggacgtagca ccaatcaact ttccggcggt gaatggcaac
    11161 gcgtacgtct tgctgcggtg gtgctgcaaa tcacaccaca agccaatccc gcaggccaat
    11221 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    11281 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    11341 accacacatt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    11401 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    11461 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    11521 atcttgaaaa gcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    11581 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    11641 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    11701 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    11761 cggtgtggac tgttcgggat ttgtggtggt gacgatgcgc gatcgtttcg atttgcagct
    11821 gccccgagaa accaaagaac aagcctctat cggcacgcaa attgataaag acgagttgct
    11881 gcctggtgac ctggtctttt tcaaaacggg ctccggacaa aatggtttgc atgtaggtat
    11941 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    12001 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    12061 aaattggacg gcgatgatac atcgccgtcc atttactcca gaatacagta gtattttgca
    12121 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggaccc
    12181 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    12241 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    12301 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    12361 ccagcaagac cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggtacggt
    12421 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    12481 ttcagagcag ttggaattac taaaatcatg ccaacatttt tttattcaac ataaactctt
    12541 tgcctggctt aatttaacgc cacaagtggc tacattatta ttagagaggg ataattacgc
    12601 aggcgaacta ttaaaatatc cgtttattga actgttgatt aatgagaatt acccgcatct
    12661 caatgaagga aaagataatc gcggtctttt atccttatcg caagtgtatc cccttgtgtt
    12721 aggtaatttg ggagcaggca atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    12781 cgtcatgctg gataaaagtt ttattcagca acaaatcacg caccgctcct tcgaaccgtt
    12841 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12901 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12961 cctgtggcct gccgttccca taaatcagat aacgacgttg gttcagcgat aaccctcctg
    13021 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacaggagg atctatgacc
    13081 ctgtctttta ttacccgctg gcgcgatgaa ttgccagcaa cctatacagc actttcccct
    13141 acgcctttaa ataatgcccg gctgatttgg cataataccg aactcgctaa cacgctgagt
    13201 attccatcgt cgctgtttaa aaatggccca ggtgtctggg gcggcgaaac cctactgcct
    13261 ggtatgtcac cactggcgca ggtttacagt ggtcatcagt tcggcgtctg ggcggggcaa
    13321 ctgggtgatg ggcgcggcat tttacttggc gagcaactgc ttgctgatgg cactacaatg
    13381 gactggcatc tgaaaggtgc tggcctgacg ccttattcgc gaatgggtga tggacgggcg
    13441 gttttacgtt cgacgatacg agaaagtctc gccagtgagg cgatgcatta tctgggcatt
    13501 ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc cagtgtatcg ggaaacggtg
    13561 gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc tgcgctttgg tcatttcgaa
    13621 catttttact atcgccgcga gccggaaaag gttcgtcagt tggctgactt tgccattcgt
    13681 cattactggt cacatcttga agatgatgag gacaaatacc gtctctggtt taacgatgtt
    13741 gtcgcacgta ccgcatcgtt aattgcccaa tggcagacgg tcggctttgc acatggggtg
    13801 atgaataccg acaacatgtc gctgctggga ctgacgcttg attacgggcc gtttggtttt
    13861 cttaatgatt acgaacccgg ttttatttgt aatcactcgg atcatcaagg gcgttacagc
    13921 tttgacaatc aacctgctgt cgcgttgtgg aatttacagc gtctggcgca gacattgtca
    13981 ccatttgttg ccgtagatgc cctgaatgag gccctggaca gctatcagca ggttttgttg
    14041 acgcattatg gacaacggat gcggcagaaa ctgggcttca tcacggagca aaaagaggat
    14101 aacgcgctac tgaatgaatt attcagtctg atggcgcgag agcgcagcga ttatacccgc
    14161 acatttcgca tgctgagtct gaccgagcag cacagcgcgg cgtcaccgct acgtgatgag
    14221 tttattgatc gtgcggcatt tgatgactgg tttgcccgtt atcgggggcg tttgcaacaa
    14281 gacgaggtta gcgatagtga gcgtcagcag ttgatgcaaa gcgttaaccc cgctctggta
    14341 ttgcgcaact ggttggcgca acgggcgatt gaagcggcag aaaagggtga tatgacggaa
    14401 ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg acagagatga tgactatgtc
    14461 agtcgtccac ctgactgggg taaacggctg gaagtcagct gctcgagtta aaggctactt
    14521 ggtcaacagc agcttgccag cctgagtttt acggagcaga tattcttgcc cgtcatgatc
    14581 gataatcagt ttgccatccg gacctaacag tgtctggctg gaaattcgcc gaatagtctg
    14641 cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg agttttctgc tatccgtata
    14701 acgcataaaa attaataata attgatataa caatgattct tattatcaat attatggctg
    14761 accagagcaa gcagtaatgt gttgagaaga caacaagttg cgcagaaatg cccatcccat
    14821 caggaatggg cacgcattca gaagcgggta tctaccgcag aggcgagttt ttcgaccagg
    14881 cgttcggtat cttcccagcc cagacacggg tcggtaatgg attgaccgta agtgagcggc
    14941 tgactgccga cgattttttg cgttccttcg cgcaggaaac tttccgccat aattccagca
    15001 atcgccgtag agccattgcg gatttgctga caaatatcct cacaaacttc taactggcga
    15061 cggtgctgct tttggcagtt accgtggctg aaatccacca ccagatgttc aggtaaatca
    15121 aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg catgataatt cggttttttg
    15181 ccgccacgca taataatgtg gccatacggg ttgccgctgg tctgatagat ggtcatctga
    15241 ccatttttgt ctggcgagag gaacatatgg ctggcgcggg ctgcgcggat agcatccaca
    15301 gcaatccgcg tattgccatc ggtaccattt ttaaaaccta ccggacagga gagtgccgaa
    15361 gccatttcac ggtggatctg actttcggta gtacgtgcgc caatcgcgcc ccaactgatt
    15421 aaatcagcaa taaactgacc ggtcaccata tcgaggaact cagtcgcggt tgggacgccc
    15481 agctcattta cctgtaaaag taatttgcgc gccagctcca gaccgtgatt tacccgatag
    15541 ctgccgttta aatctggatc ggagattagt cctttccagc cgacaacagt tcgtggtttt
    15601 tcaaaatagg tgcgcattac gatttccagc cgtgactggt actggttgcg cagcgactgc
    15661 agacgggtgg cgtactccat tgcagcggtg agatcgtgga tcgagcaggg gccaataatg
    15721 accaacagtc gcttatcttc accattgagt attttttcaa ttctgcggcg tgagtcggtg
    15781 acatgggtgg cgacgccagg cgttacggga taccgtagcg cgagttcggc gggcgttacc
    15841 aggctctcaa tacgcgcagt acggagttcg tcagttctgt tcattacaag tctcagaaat
    15901 ttgttgcttc tacggcagga ctgccgggaa gtgatgcgca tcacgataaa ccaatcgctg
    15961 ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg ttagcatgat aacaaaaaaa
    16021 taagctaatg cactagttct ctagtacatt cggcgactaa ggcccatgat atcgaggatc
    16081 ttggtggcaa tctcttctac cgaataattg gtactgttaa tccacgggat ctgattttta
    16141 cggtacaagg cttccacttc cgcgacttcc atcctgcact gacgaagcga ggcatagcga
    16201 ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt ccgggtcgat agtcaggccg
    16261 aacaatttat gctgaagcgg tttgagcgac gcgggtagca ccagattatc catatcgtcg
    16321 gcaataaagg ggtagtttgc cgcgcggata ccaaattgca ttgccagata cagactggtg
    16381 ggggttttac cacagcgaga aacaccgagc aggatcacct gagcctgatc cagattgcgc
    16441 aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg ccgcaatgcg cgcatcatat
    16501 ttattgagat tattagggtt aaggccatgg gtacgatgag caatcggcgt tggatccagt
    16561 ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga tatcctggca aaaaccttca
    16621 ctttgcaaga tgatggcgcg aatctccggc aacacgatgg agtagaagac cagcgggcgc
    16681 acgcctgtct ggtgataaat tgcgtcaatc tgatccttca ctgcccgtgc acggctctca
    16741 ttttcgacaa acggcagcgt gatgctgctg atagtgacgg gaaattgtga cattactgcg
    16801 tgtcctaata cctccgcagt tattgccgta ccatcagaaa tataaaaaac gtggcgatca
    16861 acagcattat ccattttgtt cttcccgtga tgcagacata attctatgaa agcataaatt
    16921 aaaaacacac agaagcgtag aacgttatgt ctggtttata aaatgaacct tcaattttat
    16981 tttttatgaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    17041 attgtcgaag agagcagatt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    17101 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    17161 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    17221 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    17281 gggcaaaaat gcctccctgg gtgaaatgat tactaacctt tccggaatgg gtgtttccgt
    17341 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    17401 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    17461 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    17521 aaacgccatc cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    17581 ggtgcgctcc tccgccaccg cagaagatat gccggacgct tcttttgccg gtcagcagga
    17641 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    17701 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    17761 tgtggcgctc tccgccggtg ttcaacggat ggtgcgctct gacctcgcat cttctggcgt
    17821 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17881 gggccttggt gagatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17941 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    18001 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    18061 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    18121 ggccgtacaa attgagaaac actacggtcg cccgatggat attgagtggg cgaaagatgg
    18181 ccacaccggt aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    18241 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    18301 cggtcatcgc atcggtgcgg gtccggtgaa agtcatccat gatatcagcg aaatgaaccg
    18361 catcgaacct ggtgacgtgc tggtcactga catgaccgac ccggactggg aaccgatcat
    18421 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    18481 cgctcgtgaa ctgggcattc cggcggtagt gggctgtggt gatgcaacag aacggatgaa
    18541 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    18601 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    18661 agtgatgatg aacgtcggta acccggaccg agctttcgac ttcgcctgtc tgccgaacga
    18721 aggcgtggga cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    18781 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18841 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18901 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18961 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    19021 cggcttccgt ggcgcgggac gctatgtttc cgacagcttc cgcgactgtt tcgcgctgga
    19081 gtgcgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    19141 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    19201 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaattc cgtccaacgc
    19261 cttgctggca gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    19321 gacacagctg gcgctcggtc tggatcgtga ctcaggcgtg gtgtctgaac tgttcgatga
    19381 gcgcaacgat gcggtgaaag cactgttgtc gatggcgatt cgtgccgcga agaaacaggg
    19441 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    19501 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    19561 aagcctggct gaactgaaga aataaaataa atacccggcg gcgttttgtc gccgggtcca
    19621 tttctatatc cccacctttt aacaacctac ttcaggcaaa cgcgctcgtg caatataaaa
    19681 agcttccgag catcatctga ataattggct ggtgttcttt ctgattttaa ttcgtttgag
    19741 atcttctcca cagaataatt ctcgatacca ttttttctca aagtcgaatg tgaccactcg
    19801 tcaatttcct taagcatatg cgtaattttt tcgtccagag ttcgttggat tgcacgaagt
    19861 cactggcgag tcccgttttt atactgtctt caaccacagc ataaaaagag atatggctat
    19921 gaaaaattac cgcaccgtta ttcagacata agcgcagatt atgtaattca gagaatttat
    19981 tcgaactaaa gccgattttc tgatttgtct ggctgtacag aaaacaggat aaattccctc
    20041 cacgagcgga gggaaacagc tacagggatt gcatatgagt tgctatttga aagtcctgat
    20101 ttgtgcgatt gtcgcactcg ctatatggtt ttcccccgtc cccgacgggt taacaccctt
    20161 aacctggcac attctggcaa tattcatcac cactgtggtg gcgtttattc tgcaaccgtt
    20221 gccggtaggc gctatcgcgt taatagccat cagttttatt atgcttaccg gcatgatgaa
    20281 aaccagcgaa gccttaaaag gtttcagcag caccacggta tggcttattg tcgccgcatt
    20341 cctgtacgcg aaaggattca tcaaaacggg gttaggacga cgtatcgcct atttactgat
    20401 tcgcggattt ggcggcagct cgttgcgtct gggttatacc ttagcgctaa gtgatatgat
    20461 tatcgcgccc gcaacgccat caaacacagc gcgagcaggg gggattttgt tcccgattgt
    20521 tcgcagtgtc tctaacagct ttggatctga accggatcaa gggccgcgta aaatcggcgc
    20581 ttatctgatg cagacggtgt tccattccaa ctgtctctca tcctcgatgt ttatgacggc
    20641 cagcgcgcca aatgcgctaa ttgtatctct ggcggccagc accttatttg ttgatatctc
    20701 atgggggatg tggacgctaa gtgcactggt accgggcatt atcgctttta tcactatgcc
    20761 gttggtcatt tacaaacttt acccgccaga gatcaaaaag acgccagaag ccaaagcgtt
    20821 ggcacaggaa gaacttatca aaatggggcc ggtaacccgt gatgagcgcg tgacgattgg
    20881 cattttcctg ctttcattat tggcctggag tacatcgaaa tggactggcc tggatgcgac
    20941 ggctgtcgcg ctttccggcg tctgtttaat gttgatgacc cggattatca cctggcagga
    21001 tgtgcagtca gagaaggggg cctgggacat tctggtctgg ctgggagtca tgatctgtat
    21061 ggcagataag ctcaatcagc ttggcctgtt caaatggttt gccgtcacaa cctcggcgct
    21121 gttcaccggc attccctggg aaataacact gacggttttg ctgatagttt actgttattc
    21181 ccactatttc tttgccggaa gtacgccgca tgtagtggcg atgtatgccg cttttggcag
    21241 cgtctcggtt gctgcaggcg caccgccgat gatggcagct ctgtcactgg cgttcgtcac
    21301 taacctgatg agcggcattt cgcattatgg caacggaccc gcagtgatct attacggcgc
    21361 aggctatgtc tcgcaacgag aatggtggcg gctgggcttt atcgtcatgt tgttgaacat
    21421 tgtcatctgg ttcggactgg gggcggtgtg gtggaaaatt cttggcctgt ggtaagtggt
    21481 ctgcggcaaa catcaatcaa ggaatagtgt atgaacttaa aagatattca ggtggcgatc
    21541 gtcggcaaat taagcgaccg cttaatggct aaagttgaat ctaccttcac cgcatatcgc
    21601 ctgtgggaac tgaccgacgc agacgctttt tacacgcaga tcgcgccgga tattcaggcg
    21661 ctggtgacca gtggtaatcc ggtaatggga gcttctcggg cgctcattga aaaatttccg
    21721 aatctgaaga ttattgccag caacggtgtg ggctatgacc ctattgatat cgtggcagca
    21781 agggagcatg gcgttatcgt caccaacacc cctggcgtgc ttaatgattg tgttgcagac
    21841 attggcatgg cgttgttact caatgttgcc cgacgtatta acattgccga tcgttatgtt
    21901 cgtgacggcc gttggccaaa cgaagggcgt ttcccgatgg cgacgaaagt gagtggtaag
    21961 cgttgcggaa ttgttggtct ggggaacatt ggtaacgctg tagcccgccg tgcagcagca
    22021 tttgaaatgg aaattcacta tttcgaccca aaaccgcata acaaacctgg atggacggcg
    22081 catgattcgc tggtttctct ggcgcaagcg gtggatttcc tcgttctgac gctgccaggc
    22141 ggggcaagta cacgcggcgt tattgacaaa actgtgctgc aggctctggg ggggaccggg
    22201 catttgatca gtatttcccg gggttcagtg gtgaacgaaa ctgatctgat tgatgctctg
    22261 caaaataaca taattgctgg tgcggcactg gacgtttatg ccaacgaacc acatgtgcca
    22321 gaacaattaa tgtcgctgga taacgttgtc cttacgccac atatcgccag tggtacgagc
    22381 gaaacgttta atgcgatggc ggatttggtt tttgataact tacaggcttt ctttagcgga
    22441 cagccagtta ttacccctgt tactcaataa ggcggaaaat atgatttatg gtgatatcac
    22501 ttatcttagt gaacaacaaa aacgaacatt tcatcctgtc atcgctcgtg tcattgagta
    22561 tctttcacaa catgatctga atagtgttcc tgtaggggag aaggttttta ttcaggatga
    22621 ctcaattttt tataccgtgg ttgaaccgat gactaaacca ctacatgaat gccgaccaga
    22681 agttcatcgt aaatatcttg atgtccattt cttaattgcc ggagaagagc ggatttgtgt
    22741 ggattttgat gatggcgaaa actcggttgt agaaagccac ctgggcgacc gtgacgttat
    22801 tttttatgct gatgaacgta ttaatgatcg gataatcacg ttgacaccag gacagtttgt
    22861 ggtggtcttc ccggaagagg tacatcgggc atgttataaa gtcgataacg tggagaaaat
    22921 caaaaaaaca gtcgtgaaaa tcgacatgag tctattatag gtggagctgg ttatgacaaa
    22981 aattgccctg gtgacagggg gaagtaaagg tatcggctac gcggcagcaa gggcgctcta
    23041 tgacattgga tatactgtta ttgttgccgc gcgtaacgaa gaaaccttac agcaatgtgc
    23101 acaagaatta gccaaggaac gctttattcc tctggttgct gatgtaacgg atgagtccgc
    23161 tgtcgttgca ctatttgccg agattaaggc tcgttttggt cgactggatc tcttgttcaa
    23221 taatgcgggc aataatagtc cggcaaaaag tattgaagag gttgagttta ctgagtggat
    23281 acgcgtattt aacgccaata ttcactcgac atttttgtgt tcgaaagagg cgattaaaat
    23341 tatgaaagcg cagcagccaa tgggaggacg tattattaac aatgcgtcga tttccgcact
    23401 gacgccgcgc ctctatactg cgccatacac ggcgtcgaaa catgctgtta ccgggttgac
    23461 caaggccatt tccctggatt gtcgcaaatt taatatcgct tgcgggcaaa ttaatatcgg
    23521 caatgcggaa acgacattat cgaatcggat gcgtaagggc gtttatcagg cagatggcgc
    23581 tattaaagcg gaagatatga tgaatgttgc tgatgttgca gcagccattc agatgatggc
    23641 ccaattgcca gtaaccacga atgtgctgga aataacgatt atggcaaata atatgccata
    23701 tgtcggacgt ggttaatcgg gattgaataa agcaccttgc gtataaggtg ctttattatt
    23761 tatgacttat gcctcaacgc atgattgttc cagccgttga acaatatctt tccgtaacaa
    23821 aaacttttgt attttacctg aggcagtgcg cggtagtttt tcgattacca cgatatgttc
    23881 aggatattta tattttgcga cccgtttacg gctaaaaaaa gccaccacct cttccagcga
    23941 taatgaatga tgcggcgctt tcagcacgac ataagcgcat gatcgttcac ctaaacgttc
    24001 atcgggcatt gcaaccacac aggcatcgtg aattttagga tgctgcaata aaatatcttc
    24061 cacttcacgg ctgctaatat tttcgccgcc gcggacaata atatcttttt tgcgtccggt
    24121 aatttttata tagccagcct catccatacg gcagagatcg ccgctgtaat accagccttc
    24181 ttcatccagc gcacgggcgg ttaattcagg ttcatcaaaa tatcccataa acacattggg
    24241 gccacgcgag gcttcttcac cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac
    24301 cactttaatc tctacacctg cggcagcgta accatcggtg tgcataatgc gcgacaaagg
    24361 atcatcgaga ttcaccaccg catgcggcga actttctgtg gaaccataaa cacttaataa
    24421 tttaatgccg cgctgctggc attcacgcgc cacttttttg gggattgtgg taccgccgca
    24481 aagaaagaat cgcagcgctg aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc
    24541 atagacaaac ggcgttgcgc cgagcataca ggtgcaacgc tgctgctcaa gcagcgcgag
    24601 acacgcatca ggagtgaaaa tatctaacaa cacgctgcga gcgccaatta agaatggtgc
    24661 cgttacgcca tgcagaaagc ccgttgcgtg gccgagtggc gcaggcatca taaagacatc
    24721 ctgccaggtc agattcagcc gcgcgcaata agcccgctca ctggcgagaa tattgttatg
    24781 cgttagcatc acgccctttg gcagaccctc ggttccggag gtaaacagca ccgcagctaa
    24841 ttcatcgccg tgggtcgtta tcgccgtggt cagtggggta ttgtcggcga taatctgact
    24901 taatgagagg gaagaggtgg cgggagccag tttgtccacg ccgacaattt gttgtagttg
    24961 tggaagctga ttttgcagcg gcaggattaa atctaccgga cgcgtttgtt taaacaacgt
    25021 cggtgcaaag aacatttttg cctgacactt attgagcacc cataccagtt ctgcttcccg
    25081 ccaggaaggc aacagcggta cggaaaccgc accgattttc aggcaggcaa gatagataac
    25141 ggtaaattca caccagcctg gcagttgaaa tgcgatgcga tcgcctgatt caataccctt
    25201 cgctaacatc cagtttgcca gacagctcgc ggcgtgattg agcgcgctat aggtgtacga
    25261 tgctccatga ttatcgacca cggcaatttt gtctggcatc gcacgagcgg tctgctgcca
    25321 gtaatcggcc agcgaagcat cgccccatag cccttgctga cgatacgccg cacgacgttg
    25381 ttcgttaaac gttaatgtca ctttcatttt ggactctcga aacagaacat caggcccgag
    25441 atgcgggcct gtgggatgca tgattagccg tagcgaaaat caataccgaa ggtgcctgcg
    25501 ggatattgcc actgttcgag aatagtgtta ccgcacagca cccgacaggt gccgcactcc
    25561 agacaaccgg cagaatcaaa atgaatgttg ccggcgtcat cctgcttata aagtccggca
    25621 gggcaggctt tcattaattt ttgaaattca ttgatatcgg gatttgccgc caaaatgata
    25681 tgcgggtggc cctcatcaac atggaattta ttgacgccta atttgatgtc gacgttaacc
    25741 gtagtgttct ggctcatagc gcggttgctc ccttaatgcc atctttcagc aagttgatta
    25801 gcccaatttt cttcgcgtgt cccatgatca ttttgcgtac cggctggttt ggtttgccgt
    25861 caatggtgaa catctcgttc atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc
    25921 gcgggttttc catcagcgcc gggatcttgc gaaaatgctg catatcacgc atgacgcagc
    25981 tttgttccag ctcacgtttg tattgcgcaa gactgctggc ggagaaatcc gcgcgttctt
    26041 tggcggcgat caccgttgtg gcggcagcct gagccgatgc aatggctaaa tccatgccgc
    26101 ggaccgtaaa acccaaattc aggcagaagc ctgcggcgtc accaacgatc atcacgccct
    26161 cgttaaccag ttgcggcacc attgccagac cgccttccgg caccatatgc gcggaatatt
    26221 caagcagttt gccaccgcta atcagcgggc gaatggcggg gtgttgttta aaatcttcca
    26281 gcatttgcgg cacgcttttt tgcgcatggg cgatatcacc caatccacaa accagcccca
    26341 aggatatgga atccttgttg gtatagagaa atcccccgcc catcaggccg tcagaagggg
    26401 aaccggcaaa cagccaggcg gcaccttcct cgcccgtaat attaaagcga tcgttgatct
    26461 gttctggtgt gaggccaata acctctttaa caccaacagc gtaatgatgc ggatcggaag
    26521 cgggaaccat tcccagcgag cggccaagca tcgagttaac gccattagcc agaatcacca
    26581 cattcgcttc gagaatatca tccccagcct gcacgccagt gaccttgttt ccttcacgaa
    26641 ccagcgtatc gacgcgaacg cccgggataa actgtgcgcc cgcctgctcg gcttgttcca
    26701 tcaaccacgg gtccagacga ttacgcaata cggtataaga tgcgtgttgc ggaacatctg
    26761 gttgctcgcg gtgaaaatcg agggtaacgg cgctctcttc ggttaagaag gagattttct
    26821 cgcgtgtgac cttgcgttct accggcgctg atgctgcaaa gcctggaatg attgcttcaa
    26881 gtgtgtgggc ataaagacgc ccgccggtca tgtttttaca tccggcactg tcgccgcgtt
    26941 ctatcaccag gacatccagc ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa
    27001 cgcccgcacc gaccacaatg gcatcaaatt tgtcatccga catgcgttat gctcccttcc
    27061 cgctgcgttt acagcgggag atgtggttga aaggggaatt agcggctcaa ctggctaatc
    27121 agggcaggga cgactttgta gatatcgccc accagaccgt agtcggcata gttgaagatt
    27181 ggcgcatttt tatctttatt gatggcgaca atcacttttg cgccgttgcc gccaaccata
    27241 tgttggatct gcccggagat ccccagcgtc aggtagagat cggatttcag caacacgccg
    27301 gagacaccga tataacgttc acgctccatc cagttctcgc cttcggcaat tggacgtgaa
    27361 cagccgactt cagcattcag caccgccgcc agttcgtgga ccattttcag gtcatcctgc
    27421 gccgcaagac cacgaccgac gccaaccaca cgttttgctt tgctcaggtc cacgctgctt
    27481 gcggctttcg cacggcgttc gcgacagaga atttcatgac gcggagcaac ataaggtacc
    27541 gtttctgtcg ggcactgatg agaggtatca ctggtgcacg gttcctgaac accgggtgca
    27601 agggtaatga tcgctagcgg gctgttgatc ttttcctgag cgaacgctaa cccgccatac
    27661 atccggtgtt cggcgcaaat gtgaccatcg acaatatcca ccgccgtggc atcgttcacc
    27721 agcgccgcat tcagttgcac acttaaccgt gctgccagag ctttaccacg tttcgtcgcg
    27781 gccagtagca acatagcggg gtgtttatct ttcagcagag cagcaatgct ttcggcgtaa
    27841 ttttcagtgc gttgcagcgc gtcgttttgc tcaagaacat aaatacattt tggaccataa
    27901 ggcataactg cctgcgcctg gtcggtattt tgtacaatgg catacacctg ttggccccat
    27961 tgctgagcgc cgccaaacag ttcagcataa cgttcaggat tatcgctaaa gacccagacg
    28021 ctgtttaatt gactcatcgt ttcttctccg taggcttcag ttcagggctt tcttcagatg
    28081 ttccgccagc tcggcaatgg cctccggcga atcgttatcg agaatgatgt gcttacgttc
    28141 tgtttgcggc ggtacgcgaa tgccggtcag ttcagcaagt ggtgcgctct ggctccagcc
    28201 aatatcactt gcctgccact gatttaccgg ttttttaccc gcgccgagaa tggctttcat
    28261 cgaaggaatg cgtggcacgt taatatcgga ggtgacgcag agcacggctg gaacagagag
    28321 ttcaataact tcaacgtcat cttcaagcgt gcgttcaatc accagtgtat tgccctgacg
    28381 ctgaatagca ctcactgcat taatcaccgg aagttgcaga atttctccga ccagcaagcc
    28441 aacctgctgg gcataaaggt cgccggaacc ttcaccaaag atcagtaaat cgaagccgat
    28501 cttttcaact gctgccgcca gcgcttttgc ggtatcgaga ggcagtgcat gctcaagttg
    28561 tgcatcctgc accatataca ggctgtgcgg cccgcgggat aacacgtctt tgcgcacttt
    28621 cgagttctgc aacaatgagc cgccaacggt cagcgcggct atctcatcgt catctgttgc
    28681 aagctggctt gcagcttcaa tggcattgag atcgaactgg ctgattttgg cgtcggcatt
    28741 gtcgaaattc agggtgtatt ctggagtgac aacaatgtcc tgttcttcag gtaccagctt
    28801 aaagcaggtt attattttca tgatttctcc tgtgagtcgc atacggaacc atctccgatg
    28861 gaaggtaaaa atggcctgaa aatagtgtgc gatagcggtg cggggttttc gacggagcaa
    28921 gctgttttcc ttgccaacaa tcttgcaaaa aaaatgcgat cgctaaaaca aaagtgcaat
    28981 ttgcggcaat atagttattc catgaccgta caatcttgca ttcagtgtaa ataatcttgt
    29041 ttagctctcg ctaaacgcgg ttgtataacc gcaatacacg ccgtatttta tccggcatat
    29101 attgtttttg cttgctcatg gaatagggag gttgtcatgt atcaacgctg ttttgataat
    29161 gccagtgaaa cgctgtttgt cgccggtaaa acgccacggc tttcacgttt tgcatttagc
    29221 gatgatccaa aatgggagtc tggacatcac gttcatgaca atgaaaccga gctgatttac
    29281 gtcaagaaag gggtggcaag gtttaccatc gattcttcgt tatatgtcgc gcatgcagat
    29341 gatatagtgg tgatagaacg cggcaggctg catgcggtgg cctctgacgt taacgatccg
    29401 gcaacgacgt gtacctgtgc gctgtacggc tttcagtttc agggggctga ggaaaatcat
    29461 ctactgcaac cgcattcttg tccggtaatt gccgcggggc agggaaaaga agtcattaaa
    29521 accttattta atgagctaag tgtgattttg ccgcaaagta aaaatagcca aacatcttcg
    29581 ttatgggatg catttgccta tacattagca attctttact acgaaaactt taaaaat
//
LOCUS       DAZUCE010000034        29318 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZUCE010000034 DAZUCE010000000
VERSION     DAZUCE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.034"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            141..704
                     /locus_tag="ACHY03_001333"
     CDS             141..704
                     /locus_tag="ACHY03_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3904589.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            788..3427
                     /locus_tag="ACHY03_001334"
                     /pseudo
     CDS             788..3427
                     /locus_tag="ACHY03_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3450..4199
                     /locus_tag="ACHY03_001335"
     CDS             3450..4199
                     /locus_tag="ACHY03_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS3904590.1"
                     /translation="MTNLFSAKTVATTLVFLITATSLQAQASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKMPYLAQSWMEDEQGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDIN
                     KLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKVT
                     LTRSGQEFTVNNPTPYFVVISNASAQKNGNPAAGFSPMVISPKTSVPLKVKMDSVPVL
                     TYVNDYGARMPLYFKCGGDTCQVDAEQSRKG"
     gene            4215..4718
                     /locus_tag="ACHY03_001336"
     CDS             4215..4718
                     /locus_tag="ACHY03_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3904591.1"
                     /translation="MRRILLAGSGIALLACNVAWNVASAETRPITLELKVRVDAPPPC
                     TVTGSAVEFGDVLIASIDGNNYRQNAGYTLDCSGRLEDDLRMQLKGTTTTVNGETVLD
                     AGIAGFGIRIENAADNSLFSIGENNWTSFAFNSLPQLKAVPVKQSGVQLAPAEFNASM
                     TMVVDYQ"
     gene            4715..5200
                     /locus_tag="ACHY03_001337"
     CDS             4715..5200
                     /locus_tag="ACHY03_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3904592.1"
                     /translation="MKRLALLLICGVCGGVQASPDITFHGTLVSPPACTINEGRTIEL
                     EFRDVLIDSIDGNYARQEVPYTIKCDSDVRNEAWEMTLTWTGTQTSYNDAAIETDVPG
                     FGIQLLQNGQRFQLNTPLTINATDLTQKPKLEAVPVKASGATLNDGAFSAYATLRVDY
                     Q"
     gene            5197..5721
                     /locus_tag="ACHY03_001338"
     CDS             5197..5721
                     /locus_tag="ACHY03_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3904593.1"
                     /translation="MKTTTGCMLLTGLLLAASGTTLAADNLHFYGNLLSKSCTLVVDG
                     ELLAEVHFPTVSSQDLMVAGQSSRVPVVFKLKDCKGPADYQVKVTLTGTEDSEQPGFL
                     ALDPTSGAQGVGIGMEKSDGTLVPLNDTTGATFNLSDGANSLNFNAWLQAKSGRDVTS
                     GEFSASLTATFEYI"
     gene            5723..6565
                     /locus_tag="ACHY03_001339"
     CDS             5723..6565
                     /locus_tag="ACHY03_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFS3904594.1"
                     /translation="MKLLRNFMGLLTLMVLSLTAQAQTFQMYTPMGVPSFLAKIVFSL
                     EVVTPAGVYTGTYQSHNSMMLQVGNLDLISWNGPSSIPAPTVYLDHSDGLPMSSCPGL
                     SSVPATDNGWDCYSADLKVTVTGDVQGCPWLVSSQITTTDKGNVGATYVGPKVHNSVC
                     PSIPLTTYDVSWDENTVKHKKTLNLQSTGGIIEATLSTYLMESQKLCDGSLFDERGAN
                     CRFVSQMITFTASGCDDAKVTVTPVPHPITDRQLHDIVVRVDTSALQPIDSTCRFQYV
                     LNMF"
     gene            complement(6733..7284)
                     /gene="smrB"
                     /locus_tag="ACHY03_001340"
     CDS             complement(6733..7284)
                     /gene="smrB"
                     /locus_tag="ACHY03_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFS3904595.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7450..8382
                     /gene="prmB"
                     /locus_tag="ACHY03_001341"
     CDS             7450..8382
                     /gene="prmB"
                     /locus_tag="ACHY03_001341"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS3904596.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            8417..9502
                     /gene="aroC"
                     /locus_tag="ACHY03_001342"
     CDS             8417..9502
                     /gene="aroC"
                     /locus_tag="ACHY03_001342"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFS3904597.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9506..10330
                     /gene="mepA"
                     /locus_tag="ACHY03_001343"
     CDS             9506..10330
                     /gene="mepA"
                     /locus_tag="ACHY03_001343"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFS3904598.1"
                     /translation="MNKTAIALLALLASSVSLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10330..11139
                     /locus_tag="ACHY03_001344"
     CDS             10330..11139
                     /locus_tag="ACHY03_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS3904599.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFIGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11139..11687
                     /gene="epmC"
                     /locus_tag="ACHY03_001345"
     CDS             11139..11687
                     /gene="epmC"
                     /locus_tag="ACHY03_001345"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFS3904600.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDARTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11721..11999
                     /locus_tag="ACHY03_001346"
     CDS             11721..11999
                     /locus_tag="ACHY03_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFS3904601.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(12120..14126)
                     /gene="mnmC"
                     /locus_tag="ACHY03_001347"
     CDS             complement(12120..14126)
                     /gene="mnmC"
                     /locus_tag="ACHY03_001347"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFS3904602.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLSLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPEELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETVYSEDDQQQNRQRLIDCFPQAQWAKEVDIREKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVYDDLFMLAALGSRGLCSAP
                     LCSEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            14285..15505
                     /gene="fabB"
                     /locus_tag="ACHY03_001348"
     CDS             14285..15505
                     /gene="fabB"
                     /locus_tag="ACHY03_001348"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFS3904603.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15780..16958
                     /locus_tag="ACHY03_001349"
     CDS             15780..16958
                     /locus_tag="ACHY03_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HFS3904604.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16955..17950)
                     /gene="flk"
                     /locus_tag="ACHY03_001350"
     CDS             complement(16955..17950)
                     /gene="flk"
                     /locus_tag="ACHY03_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFS3904605.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            18049..19185
                     /gene="pdxB"
                     /locus_tag="ACHY03_001351"
     CDS             18049..19185
                     /gene="pdxB"
                     /locus_tag="ACHY03_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFS3904606.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     ACLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            19251..20264
                     /locus_tag="ACHY03_001352"
     CDS             19251..20264
                     /locus_tag="ACHY03_001352"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFS3904607.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            20264..21076
                     /gene="truA"
                     /locus_tag="ACHY03_001353"
     CDS             20264..21076
                     /gene="truA"
                     /locus_tag="ACHY03_001353"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFS3904608.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            21159..21818
                     /locus_tag="ACHY03_001354"
     CDS             21159..21818
                     /locus_tag="ACHY03_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFS3904609.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            21974..22888
                     /gene="accD"
                     /locus_tag="ACHY03_001355"
     CDS             21974..22888
                     /gene="accD"
                     /locus_tag="ACHY03_001355"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFS3904610.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            22958..24226
                     /gene="folC"
                     /locus_tag="ACHY03_001356"
     CDS             22958..24226
                     /gene="folC"
                     /locus_tag="ACHY03_001356"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFS3904611.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGKELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLIPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            24216..24878
                     /gene="dedD"
                     /locus_tag="ACHY03_001357"
     CDS             24216..24878
                     /gene="dedD"
                     /locus_tag="ACHY03_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFS3904612.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            25410..25898
                     /gene="cvpA"
                     /locus_tag="ACHY03_001358"
     CDS             25410..25898
                     /gene="cvpA"
                     /locus_tag="ACHY03_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFS3904613.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            25935..27452
                     /gene="purF"
                     /locus_tag="ACHY03_001359"
     CDS             25935..27452
                     /gene="purF"
                     /locus_tag="ACHY03_001359"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFS3904614.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            27547..28116
                     /gene="ubiX"
                     /locus_tag="ACHY03_001360"
     CDS             27547..28116
                     /gene="ubiX"
                     /locus_tag="ACHY03_001360"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFS3904615.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVVMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            28382..29164
                     /gene="argT"
                     /locus_tag="ACHY03_001361"
     CDS             28382..29164
                     /gene="argT"
                     /locus_tag="ACHY03_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFS3904616.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKEFAFAGSSVKDKKYFGDGTG
                     VGLRKDDTELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
BASE COUNT         6749 a         7504 c         8058 g         7007 t
ORIGIN      
        1 aatatcttct tatgcgacct attttcttgt tagttagatc ggatagctta ttcaatggct
       61 gggtataaat aattgctatt gactgaatca atgtattgca atgtttatat ttttaattat
      121 ttatggttta aggacaaata atgagtaagt ttgtaaaaac agctattgct gcaacaatgg
      181 taatgggtgc gttcgcttct acttcaacaa tcgccgctgg caacaatggt acagcacgtt
      241 tctacggcac cattgaagat tccccgtgct ccatcgttcc tgatgatcac aaactggaag
      301 ttgatatggg tgacattggt tcagggatcc tgaaaaacaa cgggacttct acaccgaaag
      361 ctttccagat ccatctgcaa gactgtgtgt ttgacaccca gacaacgatg accactacct
      421 tcaccggtaa cgcgtcttct accaacagcg gcaattacta caccatttac aataccgata
      481 ctggtgcggc atttaacaat gtcagcctgg ccattggtga cgctcaggga acctcttata
      541 aaagcggcgc gggtatcgaa cagaaaatcg taaacgatac ggcgaccaac aaaggcaaag
      601 cgaagcagac gctggacttt aaagcctggc tggtgggcgc tgctgatgcg ccagatttag
      661 gtaacttcga agccaacacg actttccaga ttacttatct ctaagtgata ttccccatag
      721 ctttcgggct atggggtttt ttaaatggaa tttgcgccaa ttataataat tacggatgga
      781 tacgtgtatg cctgaccatt ctcttttccg attacgggtg cttccctgct gcattgcgct
      841 ggcaatgtcc gggagttatg tcaatgcctg ggctgaagat gaaattcagt ttgactctcg
      901 ttttctggaa ttaaaagacg acacgaaaat cgatctgaag cgattttcca gccagggata
      961 tgtcgaaccc ggaaaataca atttacaggt tcagctaaat aaacaaccgc tggcggaaga
     1021 atacgatatt tactggtacg cctctgagaa cgatgccagt aaaacctatg cctgcctgac
     1081 gcctgaactg gtcgcgcagt ttggcttaaa agaggatgtg gcaaaaaacc tgcaatggat
     1141 ccacgacggc aaatgcctga aacccggtca actggaaggc attgatatta aagctgacct
     1201 gagtcagtca gcgttagtca tttcattacc ccaggcttac cttgaatata ccgacaccga
     1261 ctgggaccca ccttcacgct gggatgacgg tatttctggt ttaattgctg actacagtat
     1321 taccgcccag acacgacatg aagaaaatgg cggggatgac agcaatgaaa tcagcggcaa
     1381 cggaacggtt ggggttaacc tcggcgcatg gcgtctgcga gctgactggc agaccgacta
     1441 ccagcacagc aaaagcaatg atgaagatgt tatcaacggt gatgacacgc aaaaaaactg
     1501 ggagtggagc cgctactacg cctagcgggc tttaccgtcg ctaaaagcca aacttggcct
     1561 tggcgaagac taccttaatt ctgatatttt cgacggcttt aactacgtgg gtggcagtat
     1621 cagcaccgac gatcaaatgt tgccgccgaa tctgcgcggc tatgcgccgg atatctccgg
     1681 cgtggcgcac accaccgcga aagtgaccgt cagccagatg ggccgcgtga tttacgaaac
     1741 tcaggtcccg gcggggccgt tccgtatcca ggatcttggc gattccgtct ccggtacgct
     1801 gcatatccgc attgaagagc agaacggtca ggtgcaggaa tatgacatca acaccgcctc
     1861 gatgccgttc ctgacccgcc ccggccaggt gcgctataag ctgatgatgg gccgcccgca
     1921 ggagtggggg catcatgttg aaggtggttt cttctccggc ggcgaagctt catgggggat
     1981 tgccaacggc tggtcgctgt acggcggggc gctggcagat gaacactatc agtcggcggc
     2041 gcttggcgtc ggtcgcgacc tgtctgtctt tggcgcagtc gcctttgata tcacccactc
     2101 gcatacccgc ctggataaag agaccgccta cggtaaaggt tcactggacg gtaactcgtt
     2161 tcgcgtgagc tattccaagg actttgatga actgaacagc cgcgtcacct ttgccggata
     2221 ccgcttctcg gaagagaact ttatgaccat gagcgagtat ctcgatgcca gcgacagcga
     2281 aatggtgcgc accggcaacg acaaagagat gtacaccgcc acctataacc agaacttcag
     2341 ggatgccggt gtgtctgttt atctcaacta cacccgccat acctactggg atcgcgacga
     2401 gcagaccaac tacaacgtta tgctctcgca ctacttcaac ttgggcagta tccgcaacat
     2461 gagcatttct atgaccggat accgctacga gtatgacaac caggccgata aaggtgtgta
     2521 catatcgctc agtatgccgt ggggtgacag cagcaccatc agctataacg gcaactacgg
     2581 cagcggttcg gacagcagcc aggtagggta tttcagccgt gtcgatgacg caacccatta
     2641 ccagttgaac gtgggaacca gtgacaatca ttccagcgtt gatggctatt acagccacga
     2701 tggatcgctt gcgcaggtgg atctcagcgc caactaccat gaagggcagt acacctcggc
     2761 agggatttca ctccagggcg gggcaacgct caccgcgcag ggcggcgcgc tccaccgtac
     2821 ccagaatatg ggcggcacgc gtctgctgat tgatgccgac ggtgtggccg gtgttccggt
     2881 ggaaggaaac ggtgcagcgg tttacaccaa tatgttcggt aaggccgtgg tggcagacgt
     2941 caacaactac taccgcaatc aggcctatat cgaccttaac aacctgccgg aaaacgccga
     3001 agccacccag tccgtggtgc aggccacgct taccgaagga gccattggct accgtaagtt
     3061 ttcggttatc agcgggcaaa aagcgatggc ggtattgcgc ctgcaagatg gcagtcatcc
     3121 gccgtttggc gcggaagtga aaaatgacaa cgcgcagaac gttggtctgg ttgacgatga
     3181 cggcaacgtc tacctcgcgg gcgtcaaacc tggcgagcat atgaccgttt catggggcgg
     3241 tgtggcccac tgcgacattc atctgccaga cccgctgcca gccgatctat tcaatggcct
     3301 gttattgcca tgccagcaaa caggggcgat atctccttcg gtgcccaatg aaattaagcc
     3361 ggtgatccag gagcagactc agcaggtcac gccaatggaa ccgccagtat cggtatcagc
     3421 caattaataa cgtgattaag gaatgatcca tgacgaatct attttctgcc aaaacggtcg
     3481 caaccacgct ggtgtttctg ataactgcca ccagcttgca agcccaggca tcggtcacgc
     3541 cagaccgtac ccgtttgatt tttaacgaaa gtgataaatc aatcagcgtc acgctgcgca
     3601 ataacgaccc gaaaatgccg tatctggcac aaagctggat ggaagatgaa cagggtaaca
     3661 agattagctc gccactgacg gtactgccgc cggtgcagcg catcgattcc atgatgaacg
     3721 gtcaggtgaa ggtacagggc atgccggata tcaacaagct gcctgccgac cgcgagagcg
     3781 tgttctattt caacgtgcgt gagatcccgc cgaaatcgaa caaacccaac acgttacaga
     3841 ttgcgctgca aacgcgcatc aagctgttct ggcgaccaaa ggcgctggaa aacgtcagca
     3901 tgaaaaatcc atggcagcat aaggtgacct taacccgcag cggccaggag tttactgtca
     3961 ataacccgac gccgtacttc gtggttatca gtaacgctag tgcacaaaaa aatggcaatc
     4021 cggcagcggg attttccccc atggtgatat cgccaaaaac ctctgtgccg ttaaaagtca
     4081 aaatggatag cgtcccggtg ctgacctacg ttaacgacta tggcgcacgg atgccgctgt
     4141 attttaaatg tggcggcgat acctgccagg tggatgcaga gcagagccgc aaaggctgat
     4201 gccgggagag tctgatgaga agaattttac tggcaggctc gggcattgcg ctgctggcct
     4261 gtaacgtggc gtggaatgtt gccagtgcgg aaaccagacc catcactctg gagcttaaag
     4321 tcagggtgga tgcgccgccg ccctgtaccg ttaccggtag tgcggtggag tttggcgatg
     4381 tgctgatcgc cagcattgat ggcaacaact accgccagaa cgcgggctac accctcgact
     4441 gttcggggcg tctggaagat gacctgcgga tgcaactgaa aggcactacc accacggtga
     4501 acggtgaaac tgtgctggat gcaggcattg ccgggtttgg catccgtatc gaaaacgccg
     4561 ccgataactc cctgttttcc attggtgaaa ataactggac gtcttttgcg tttaacagcc
     4621 tgccgcagct gaaggcggtg ccggtaaaac aaagcggcgt gcaactcgcg ccagcggaat
     4681 ttaacgccag tatgacgatg gtggtggatt accaatgaaa cgactcgcac ttttgctgat
     4741 ttgtggtgta tgcggtggcg ttcaggcctc accggatatc acctttcacg gcacgctggt
     4801 gtcgccgcct gcctgcacca tcaacgaagg ccggaccatt gagctggaat ttcgcgatgt
     4861 gcttatcgac agtattgatg gcaactacgc ccgccaggag gtgccgtaca ccatcaaatg
     4921 tgactccgac gttcgcaatg aggcctggga gatgacgctc acctggacgg gcacgcaaac
     4981 cagctacaac gacgcagcca tcgaaaccga tgttccggga ttcggtattc agcttttgca
     5041 gaacgggcag cgttttcagc ttaatacccc gctgaccatt aacgccacgg atttgacaca
     5101 aaagccgaag ctggaggctg taccggttaa agcgagcggc gccacattaa atgacggcgc
     5161 gttttcagcg tacgcaacat tacgggtgga ttatcaatga aaacaacgac cggatgtatg
     5221 ctcctgacag gcctgttgct tgccgccagc ggaaccacgt tggcggcgga caatctgcat
     5281 ttttacggca atttattgag taaatcctgc accctggtgg tggatggcga actgctggcg
     5341 gaagtgcatt tccccaccgt tagcagtcag gatttaatgg ttgcggggca gtcttccagg
     5401 gtgccggtgg tctttaagtt gaaagattgc aaaggcccgg cggattacca ggtcaaagtc
     5461 accctgaccg gtacggaaga cagcgaacag ccagggtttc tggcgcttga cccgacttcg
     5521 ggtgcgcagg gggtagggat tggtatggaa aagtccgatg gaaccttggt gccgcttaat
     5581 gacacaactg gcgcgacttt caatctcagt gacggcgcga atagcctcaa ttttaatgcg
     5641 tggttacagg cgaaaagcgg tcgtgacgta acttcaggtg agttcagcgc cagcctgacc
     5701 gccacttttg agtacattta agatgaaact actacgaaat tttatgggct tgctgacgct
     5761 gatggtattg agcctgacgg cccaggcgca gacatttcag atgtatacgc ctatgggggt
     5821 tccaagcttc ctggcaaaaa tagtgtttag tctggaggtt gttacccctg caggcgttta
     5881 tacaggcacc tatcagtcgc ataattccat gatgcttcaa gttggtaatt tagacttaat
     5941 aagttggaac ggtccgtctt caatacctgc acctaccgta tatttggacc attcggacgg
     6001 cttacctatg tctagttgtc ctggcctttc cagtgtacct gcgaccgaca acggctggga
     6061 ttgttattct gctgatttaa aagtcactgt aaccggggat gtgcaggggt gtccgtggtt
     6121 agtgtcaagc cagattacta ccacggacaa ggggaatgta ggggctacct atgtcggccc
     6181 taaagtccat aactccgtct gtccgtcaat tccgctgacg acatatgatg tttcctggga
     6241 tgagaatacg gtgaagcata aaaaaacatt aaatctgcaa tccacagggg ggatcattga
     6301 ggcaaccctg tcgacctatt taatggaaag tcagaagttg tgtgatggta gcctgtttga
     6361 cgagcgcggg gctaattgcc gttttgtttc gcagatgatc acctttaccg catcaggctg
     6421 cgatgatgcc aaagtgacgg tgacgccggt tccgcatccg attactgaca ggcaactgca
     6481 tgatattgtg gtgcgcgtag ataccagcgc attgcagcct attgattcaa cgtgtcgctt
     6541 tcagtatgtg ttgaatatgt tctagcacgc cgcatccggc ggttgtgtgc tgatgcctga
     6601 tgcgacgctg acgcgtctta tcaggcctac aaatgaatga caacgtattg aatttgcaca
     6661 ctttgtaggc cggataaggc gttcacgcca catccggcat tcggtgccag tgccacatcc
     6721 ggcacttaat attcacggca actctggcgg caaccactct tccacttcaa tcaacaccag
     6781 caacgctgca tctccgccgt actctttcgg tgcctggtga aatgccatta catgcggatg
     6841 ttgcgccagc cacagcggcg tttgctgttt caaaatatgc ttcccgtgcc cgtgcattac
     6901 gcatgcgcaa aacacatgtt cacgacggca ggcggcaatc aacgcgccca gttcctgctt
     6961 ggcctgcagc tgcgtcagac cgtgtaaatc caaaaacaat tccggcgaat aatcaccgcg
     7021 tcgcaacttc ttcgcctcaa aatgactcac atccgggcga acatatttaa ccggaccttc
     7081 ggtatttaat aacggctgaa actcatcgga gaaataatgg ctggcatcag cctgctcctg
     7141 gatcaagcgt ttcaccggca cttcgctgat tttcttacgc tgcggtcggt ggacaatcgt
     7201 gtcctgctta atcttgcgag tccccgccat caactggcga aacagagcct ggtcctcctc
     7261 gctaagtgtt gttttctttt tcatttatct gtctctattt ctaattttcc tacagtttac
     7321 ccgactcgta gcgcaatcga tcgttttaaa cgtatttttc ctcactcacc gcgcgtgaat
     7381 gctgatttat cgtcatcttc atggcaaact agccgccgaa attattgcga tcatgccctg
     7441 gaggaatacg tggataaaat tttcgttgat gaagcagtaa atgagctgca aaccattcag
     7501 gacatgttgc gctggtcggt aagccgcttc agcgcggcaa atatctggta cggtcacggc
     7561 accgataacc cgtgggatga agccgtgcag ctggtattgc cgtcgctcta cctgccgctg
     7621 gatattccgg aagatatgcg caccgcgcgt ctgacctcca gcgaaaaaca ccgtattgtt
     7681 gaacgcgtga tccgccgcgt caacgaacgc attccggttg cttacctgac caacaaagcg
     7741 tggttctgcg gccatgaatt ttacgtcgat gaacgtgtgc tggtgccgcg ctcgccgatt
     7801 ggtgaactga tcaacaataa atttgccgga cttatcagca agcaaccgca gcatatttta
     7861 gatatgtgta ccggtagcgg ctgcatcgcc attgcctgtg cttatgcctt cccggatgcg
     7921 gaagtcgacg cggtggatat ctctcccgac gcgctggcgg ttgccgaaca gaacatcgaa
     7981 gaacacggtc tgatccacaa cgtcattccg attcgttccg atctgttccg tgatttgccg
     8041 aaagtgcagt acgacctgat cgtcactaac ccgccgtatg tcgatgcgga agatatgtcc
     8101 gacctgccaa acgaataccg tcacgagccg gaactgggcc tggcatctgg cactgacggc
     8161 ctgaaactga cgcgtcgcat tctcggtaac gcggcagatt accttgctga tgatggtgtg
     8221 ctgatttgtg aagtcggcaa cagcatggta catcttatgg aacaatatcc ggatgttccg
     8281 ttcacctggc tggagtttga taacggcggc gatggtgtgt ttatgctcac caaagagcag
     8341 ctgctggcgg cccgtgaaca tttcgcgatt tataaagatt aagtaaacac gcaaacacaa
     8401 caataacgga gccgtgatgg ctggaaacac aattggacaa ctctttcgcg taaccacctt
     8461 cggcgaatcg cacgggctgg cgctcggctg catcgtcgat ggtgttccgc caggcattcc
     8521 gctgacggaa gcggacctgc aacatgacct tgatcgtcgt cgccctggga catcgcgcta
     8581 taccacccag cgtcgtgagc cggatcaggt caaaattctc tccggtgttt ttgaaggtgt
     8641 tactaccggc accagcattg gtttgttgat cgaaaatacc gatcagcgtt ctcaggatta
     8701 tagtgcgatt aaggacgttt tccgtcctgg ccatgccgat tacacctacg aacaaaaata
     8761 cggtctgcgc gattatcgcg gcggtggacg ttcttccgcc cgcgaaaccg ccatgcgcgt
     8821 ggcggcaggg gcgattgcca aaaaatatct cgccgagaaa tttggtattg aaatccgcgg
     8881 ctgcctgacc cagatgggcg acattccgct ggaaatcaaa gactggtcgc aggttgaaca
     8941 aaatccgttc ttctgcccgg acccggacaa aatcgacgcg ttagatgaac tgatgcgcgc
     9001 gctgaaaaaa gagggcgact ccatcggcgc gaaagtcacc gttgtagcca gtggtgtccc
     9061 tgccggactt ggcgagccgg tctttgaccg cctggatgcc gacatcgccc atgcgctgat
     9121 gagcatcaac gcggtgaaag gcgtggaaat tggcgatggt tttgacgtag tggcgctgcg
     9181 cggcagccag aatcgcgacg aaatcaccaa agacggtttc cagagcaacc atgcgggcgg
     9241 cattcttggc ggtatcagca gcgggcagca aatcattgcc catatggcgc tgaaaccgac
     9301 ctccagcatt accgtgccgg ggcgcaccat taaccgcttt ggcgaagaag ttgagatgat
     9361 caccaaaggc cgtcacgatc cctgtgtcgg gatccgcgcg gtgccgatcg ctgaagcgat
     9421 gctggcgatc gttttaatgg atcacctgct acggcagcgg gcgcaaaatg ccgatgtgaa
     9481 gactgatatt ccacgctggt aaaaaatgaa caaaaccgcg attgcgctgc tggccctgct
     9541 tgccagtagc gtcagcctgg cagcgacgcc gtggcaaaaa ataacccaac ctgtgccggg
     9601 tagcgcacaa tcgataggca gtttttctaa tggctgtatc gtcggcgctg acacgctgcc
     9661 gatacagtcc gaacattatc aggtcatgcg taccgatcag cgtcgctatt tcggtcaccc
     9721 ggacctggtg atgtttatcc agcgtctgag tagccaggtg agcaatctgg gcatgggtac
     9781 ggtgctgatt ggcgatatgg ggatgcccgc tggtgggcgc ttcaacggcg gtcatgccag
     9841 ccaccagacc ggactggatg tcgatatctt tctgcaactg ccgaaaactc gctggacctc
     9901 cgcgcagctc ttgcgcccgc aagcactgga cttagtttcc cgcgacggta aacacgttgt
     9961 ctccacgctg tggaagccgg aaattttcag cttgatcaaa ctcgccgccc aggacaaaga
    10021 cgtcacgcgc atttttgtta atccggcgat taaacaacaa ctttgccttg atgcgggcac
    10081 cgatcgcgac tggttgcgca aagtgcgacc ctggttccag catcgcgcgc atatgcatgt
    10141 acgattacgt tgccctgccg atagtctgga gtgtgaagat caacctttac cgccaccagg
    10201 cgatggttgc ggggcagaac tgcaaagctg gtttgaacct ccaaaaccgg gaacaacaaa
    10261 gcctgagaag aagacaccgc ctccgttgcc gccttcctgc caggcgctac tggatgagca
    10321 cgtgatctaa tggaaacgtt taatagcctg tttatggttt ccccgctgtt gctgggagtt
    10381 ctcttttttg tcgccatgct ggcgggattt atcgactcga ttgccggtgg cggtgggtta
    10441 ctcaccattc cggcattgat ggcagcgggg atgtctcccg ctaatgcgct ggcaaccaat
    10501 aaactgcaag cctgcggcgg ctctatttcc gccactatct actttattcg ccgcaaagtg
    10561 gttagtttaa gtgatcagaa actcaatatc gccatgacct ttatcggctc aatgagcggc
    10621 gcactgctgg tgcaatacgt tcaggctgat gtcttacggc agattttgcc cattctggtg
    10681 atttgtatcg gcctctattt cttgctgatg ccaaagctgg gtgaagaaga tcgccagcgc
    10741 cggatgtacg gtttaccctt cgcgttaatt gccggtggct gcgtcggttt ttatgacgga
    10801 ttctttggtc cggcagccgg atcgttttac gcgctggcct ttgttacgct gtgcggattc
    10861 aacctcgcca aagccacggc tcacgccaaa ttactcaacg caacgtcaaa catcggtggt
    10921 ttactgctgt ttattctcgg cggcaaagtg atttgggcga cgggctttgt gatgctagtg
    10981 gggcagttcc tcggcgcgcg catgggttca agactggtgt tgagcaaagg acaaaaactg
    11041 atccgcccga tgatcgttat cgtctcggcg gtgatgagtg ccaaattact ttacgatagc
    11101 catggacagg agatcctcca ctggttgggg atgaactaat gaacagtaca caccactacg
    11161 agcagttgat tgaaattttt aatagctgct ttgccgatga ttttaatacc cgtctgatta
    11221 aaggcgacga cgaaccgatc tatcttcctg ctgatgcgga agtaccgtat aaccgaatcg
    11281 tctttgctca tggcttttat gccagcgcaa ttcatgagat ttcgcactgg tgtattgccg
    11341 gaaaagcgcg ccgtgagctg gttgatttcg gctactggta ttgcccggac ggacgcgatg
    11401 cccgaacgca aagccagttt gaagatgttg aagtgaagcc gcaggcgctc gactggttgt
    11461 tctgcgtggc ggctggatat ccgtttaacg tcagttgcga caatctggaa ggcgacttcg
    11521 aaccggaccg cgtggtgttt caacggcgag tacatgcgca ggtgatggat tatctaacaa
    11581 atggcattcc ggaacgcccg gcacgtttca ttaaagcact acaaaattat tatcacacgc
    11641 cggaacttac ggcggaacag ttcccgtggc cggaagcgct caactgaagc cagcgccacg
    11701 ttttacaatg aggaaagaag atgatcgcgg agtttgaatc acgcattctg gcattaatcg
    11761 acggtatggt tgaccatgcc agtgatgatg agttgtttgc cagtgggtat ttgcgtggcc
    11821 acctgacatt agccatcgca gaactggaaa gtggtgatga ccactccgct caggcggtgc
    11881 atacgaccgt tagccagagt ctggaaaaag ccattggtgc tggtgaattg tcgccgcgtg
    11941 accaggcgct ggtgaccgat atgtgggaaa acctgtttca gcaggcgtca cagcagtaag
    12001 ttgcggcgtc tggagcacca tgcctgatgc gacgcttatc gcgtcttatc aggcctacac
    12061 gtcacacgta ggtcggataa ggcgttcacg ccgcatccga caaacaatgc caaagctggt
    12121 tacccagcct taactgcttt acccttcaac aatttccgca cccataaccg attcggattt
    12181 aacgccgcca gcgtactggc atccatcgga atcggttctt cactcatctg tgccgccaga
    12241 atctcggagc acagtggggc agaacacaaa ccacgcgaac ctaaagcagc aagcataaag
    12301 agatcgtcat aaaccggcgc gcttaccgcc ttatctttct gctccgccag cgacgcatat
    12361 tccacgagtg ttgcctcata atcgggaaca ttacctacca ttggcagatg atcgcgggtg
    12421 gcacaacgca caccgcagcg cgcctctttc tccctgatat caacctcttt tgcccactgt
    12481 gcctgcggga aacaatcaat caaccgctgg cgattctgct gctgatcgtc ttcactgtac
    12541 accgtctctt cgctgccgcg atgataactg gcaccaatac aatggtgctg attcgccgga
    12601 ttttgtggcg tgagataacc gtcatagcac agcacctgct tcagttttgc cagttcgggt
    12661 gtcgtcggaa tatggctgac ctgaccggca accgaatata ctggcagaga tgatgtttgg
    12721 ctgaaacggc tgatttgatg cccgttcgcc agtaccacta cgctgtgtgt tgcttgctga
    12781 tctcctgcaa aattcaacaa ccaacagtca tccttacggg ataaatcctg taactgatat
    12841 tgataataaa tctgcaaacc ctgctgttgc gccagttcca gcacattacg ggtcagttct
    12901 gctgggcaca gccaaccacc ttgcggataa gtaatgccgc tgcaatttgt cgcaacgccc
    12961 gtaatttgtt caaccgcatt tgcctcaacg gctacagcca gttcttcggg taaatccatt
    13021 gatagcatct gtgcgatttt atgctggctt ttctcatccc agcctaactg cgtgacgccg
    13081 caccagtcat gatcaaattt aacgggtaat tggtcgtaaa accgacgagc aaaagtaaac
    13141 gcattagaga aaaagcggtt tagcgcctca tcgtgtttgc ttaataacgg atacaacgcc
    13201 ccctggcgat tgccggaagc acccagcgcg ggggcttcat ccgcgcaata aagcgttact
    13261 tgccagccgc gccgtaataa cgccagcgac aacaatgcgc tggcaatacc gccgccgata
    13321 atcgcaactt cccgtttgct gctgcccgta cggttaaacc acggcgtgga gcaggggagt
    13381 gataatgtct gttccatcac cccacaaagc atttcccgtt tgcgcccaaa gcccttacgt
    13441 ttttgcatcg tgaatccggc gtcctgcaaa ccgcggcgga caaaaccggc agatgtaaat
    13501 gtcgccagcg tgccgcccgg acgcgccaac cttgccatgg cattaaacag attttgcgtc
    13561 cacatatccg ggtttttcgc tggcgcaaag ccgtccagaa accaggcatc tactttttga
    13621 tttagagaat cgtccagttg gctggtcagt tcattaatat cgccaaacca taaatccagc
    13681 gtcacgcggc cttcatcgag taataaacga tggcaaccgg gcaagggcat tggccactgc
    13741 gcctgaagtt gttctgccca cggagccagt tccggccagt gttgatgcgc taaggctaaa
    13801 tccgcacggg tgaggggaaa tttctcaaaa ctaatgaaat gtaagcgttg taattgcgct
    13861 tgcggatgcg cttcgcgaaa ctgatcaaat gcctgccata gcgtcaggaa gtttaatccg
    13921 gtgccgaagc cgctctctgc taccacaaac agaggatgtg gatgctcagg aaagcgtgcc
    13981 tctaattggt tacctccgag aaaaacataa cgcgtctctt ccagcccgtt atcgttggaa
    14041 aaatagacat cgtcaaaatc tcgggaaaca ggtgtaccct cagcattaaa ttcgaggttg
    14101 gcaggttgta tggagtagtg tttcacgtaa gttactcgtc ttacaggcgg tggctcgatc
    14161 ttagcgatgt gtgtaaggct gcgcaaattt ctctattaat tggctgatcg gacttgttcg
    14221 gcgtacaagt gtacgctatt gtgcattcga aacttactct atgtgcgact tacagaggta
    14281 ttgaatgaaa cgtgcagtga ttactggcct gggcattgtt tccagcatcg gtaataacca
    14341 gcaggaagtc ctggcatctc tgcgtgaagg acgttcaggg atcactttct ctcaggagct
    14401 gaaggattcc ggcatgcgta gccacgtctg gggcaacgta aaactggata ccactggcct
    14461 cattgaccgc aaagttgtgc gctttatgag cgacgcatcc atttatgcat tcctttctat
    14521 ggagcaggca atcgctgatg cgggcctctc cccggaagct taccagaata acccgcgcgt
    14581 tggcctgatt gcaggttccg gcggcggctc cccgcgtttc caggtgttcg gcgctgacgc
    14641 aatgcgcggt ccgcgcggcc tgaaagcggt tggcccatat gtggtcacca aagcgatggc
    14701 atccggcgtt tctgcctgtc tcgccacgcc gtttaaaatt catggcgtta actactccat
    14761 cagctccgcg tgtgcgactt ccgcacactg tatcggtaac gcagtagagc agatccaact
    14821 gggcaaacag gacatcgtgt ttgctggcgg cggcgaagag ctgtgctggg aaatggcttg
    14881 cgaattcgac gcaatgggtg cgctgtctac taaatacaac gacaccccgg aaaaagcctc
    14941 ccgtacttac gacgctcacc gtgacggttt cgttatcgct ggcggcggcg gtatggtggt
    15001 ggttgaagag ctggaacacg cgctggcgcg tggtgctcac atctatgctg aaatcgttgg
    15061 ctacggcgca acgtctgatg gcgcagacat ggttgctccg tctggcgaag gcgcagtacg
    15121 ttgtatgcag atggcgatgc atggcgttga taccccaatc gattacctga actcccacgg
    15181 cacttcgact ccggttggcg acgtgaaaga gctggcagct atccgtgaag tgttcggcga
    15241 taagagcccg gcgatttctg caaccaaagc catgaccggt cactctctgg gcgctgctgg
    15301 cgtacaggaa gctatctact ctctgttgat gctggaacac ggctttatcg ccccgagcat
    15361 caacattgaa gagctggacg agcaggctgc gggtctgaac atcgtgaccg aaacgaccga
    15421 tcgcgaactg accactgtta tgtctaacag cttcggcttc ggcggcacca acgccacgct
    15481 ggtaatgcgc aagctgaaag attaattcgc agtaggccgg agtaggtcgg ataagacgcg
    15541 tcagcgtcgc atccgacgtt acgcgccaat gcggcatccg gcactaacgc caaagggaac
    15601 ccgatggttc cctttttcac atcattgaca atcgccgcct gttccaggca aacttcccgc
    15661 tttgtcgatt tccttctgaa aagacgtacg cgttaattcc tgccaacgca ccgtaaccct
    15721 gaaaccagag agatgagacg gggatactcc tcgccttgcg ctgcattctg gagtaatgca
    15781 tgactgctgt aagccaaacc gaaacacgat cttctgccaa tttttcgctc ttccgcatcg
    15841 cttttgcggt ttttctcacc tacatgaccg tagggttgcc gttgccggtt atcccgttgt
    15901 ttgttcatca tgaattgggc tatggcaata ccatggtcgg cattgccgtc ggcattcagt
    15961 ttctggctac ggtgctgacg cgtggttacg ccgggcgact ggccgatcaa tatggcgcaa
    16021 aacgttcggc gcttcagggg atgttagctt gcggtctggc ggggggcgcg ttgctgctgg
    16081 cggcgatttt gcctgtctcc gcaccgttca aatttgccct gttggtcatc gggcgtttga
    16141 ttcttggctt tggtgaaagc cagttactga caggcgctct gacctggggg ttaggcatcg
    16201 tagggccaaa acactctggc aaagtgatgt catggaatgg aatggcgatt tacggtgccc
    16261 tcgctgttgg tgctccgctt ggcctgttga ttcatagcca ttacggtttt gccgcactgg
    16321 cgatcaccac aatggtatta cccttactgg cgtgggcctg taacggcaca gtgcgcaaag
    16381 taccggccct ggcgggagaa cgtccatcgc tgtggagcgt tgttgggctt atctggaaac
    16441 cagggttagg tctggcacta caaggcgttg gttttgcggt tatcgggact ttcgtttcgc
    16501 tctactttgc cagcaaagga tgggcgatgg cgggctttac tcttaccgcg tttggcggcg
    16561 catttgtcgt gatgcgcgtc atgtttggct ggatgccgga ccgttttggc ggcgtgaaag
    16621 tggcgattgt ctctctgctt gtagaaacgg tgggcttgtt gctgctctgg caagccccag
    16681 gtgcgtgggt cgcattagcg ggcgcggcgt taaccggagc cggatgttcg cttatctttc
    16741 ctgcgctggg cgtggaggtg gttaaacgtg tcccctcaca agttcgcggc accgcactgg
    16801 gcggttacgc cgcgtttcag gatatcgccc tcggcgtctc cgggccgctg gcgggaatgc
    16861 tggcgaccac gtttggttac tcttcggtat ttcttgccgg ggcgatctct gcggtgctgg
    16921 gaattattgt cacgatactg tcatttcgtc ggggttaacg aaccagccag accagcatca
    16981 ggataatcgc gactaacagt atccacagcg cgggacgcgt tgccatattt tgcaacgtgt
    17041 ccatcatcgg tggaaaagga ttaagcagcg gttgcatatc acgcggatca atccctttaa
    17101 cccgccgttg ataaagctga ttaagaatat cctcggcctg ggcagaagaa agcgatgatt
    17161 gcgcagaaag gccatatttt tgctggatat atgccgataa cgccgccagt tcactggcat
    17221 ctaaaggttg ttttagcgtc atctgtagtg attccagcgt cggcgtattt tgctggctta
    17281 gcgtctgacg cgcctgcagc caggtcacca gatggttaaa cagtttcgct ggaattaact
    17341 caccatcttt caccccggaa agttccagca tcgattgcca gatctgtttg ctgggttccc
    17401 ccgttgccgc cgcaagtttg gtcaccagct gtttgagcgc attgtgctcc gccggtaata
    17461 gaggacgatc ggtcgcctcg cgctgctgcg gttgcggaat aaccatcttc ccttcctgca
    17521 acagggtgag aatggttttt aattgctccg gtgagagctg attcagcggc gtctgaccaa
    17581 agttatgacg gatataatcc gtgaccgcct gacgattatt ccccagacgt aaatactccc
    17641 ctaattgcgc taaaagctgg cgggcagaat ggcttttttg cgcggccagc agacgttgcg
    17701 ccagattatg ctcagcggca gggaagtgac gcgaaagcag cggtgaatct cccgacagac
    17761 caatatcgtg cctgataccc gcccacagtt ctgctctttg ctgttgcgtc agtgaggtca
    17821 ctttcgtcat taagctttcc agcgaagtac gttgctgact ggataaaggc tgattgcccg
    17881 cgccagacgg caggttatct ccctgacctg gtggttgccc aggaggaggg ccggaaatag
    17941 gttgtatcat tacgtatcct tatacctgaa atcttcgcaa gtatgcctgg ccgcgagatt
    18001 atggcacact tgtccggtta actctcgtct catacaggta acacaaacgt gaaaatcctt
    18061 gttgatgaaa atatgcctta tgcccgcgac ttatttagcc gtttgggtga ggtgatcgcg
    18121 gttcccgggc gtccaatccc cgtcgctcaa ctggcagacg cggatgcgct gatggtgcgt
    18181 tcggtcacga aagtgaatga atctttgctg gcaggaaaac ccattaaatt tgttggcact
    18241 gccactgcgg ggaccgacca tgtcgatgaa gcatggttga agcaggcggg aattggtttt
    18301 tccgctgcac ctggctgtaa tgcgattgcg gtggtggaat atgttttctc ctccctgctg
    18361 atgcttgccg aacgcgatgg attttcactg cacgagcgta ccgtggggat cgtgggcgtt
    18421 ggtaacgttg gtcggcgttt acaggcacga ctggaagcgt tagggattaa aaccttactt
    18481 tgcgatccgc ctcgcgctga ccgtggggat gagggtgatt tccgctcgct ggatgaatta
    18541 gttcagcatg cggatattct gactttccat acgccactct tcaaagacgg gccgtacaaa
    18601 acgctgcatc tggcggatga aaaactgatc cgcagcctga aaccgggagc gattctgatt
    18661 aacgcctgcc gtggcgcagt cgtcgataat accgcgctgc tggcctgcct gaatgaaggc
    18721 cagaagttaa gcgtggtgct ggatgtctgg gaaggcgagc cggaacttaa tgtggaattg
    18781 ctgacaaaag tggatatcgg cacgccgcat attgcaggct ataccctgga aggtaaagca
    18841 cgcggtacta cgcaagtgtt tgaagcctat agcaagttta ttgggcatga acagcacgtt
    18901 gcgctggata cattactgcc cgcgccagag tttggtcgca ttactctgca tggaccgctc
    18961 gatcaaccga cgctgaaaag gctggtgcat ttggtgtatg atgtgcgccg cgatgacgca
    19021 ccgctgcgta aagtcgccgg gataccgggt gagttcgata aactgcgcaa aaactatctt
    19081 gagcgccgtg aatggtcatc tctgtatgta atttgtgatg acgccagtgc ggcatcattg
    19141 ctgtgtaaac tgggttttaa cgccgttcat catccggcac gttaatctct tcttcatgct
    19201 ctctgctgta acattggcag ggagctttgc tatttctgga gtaaaccacc atgtctgaag
    19261 gctggaacat tgccgtcctg ggcgcaactg gcgctgtggg cgaagccctg cttgaaacgc
    19321 tggctgaacg tcagttcccg gttggggaaa tttatgcact ggcacgtaac gaaagcgcag
    19381 gcgaacaact gcgctttggt ggtaagacaa tcaccgtgca ggatgccgct gaattcgact
    19441 ggacgcaggc gcagctggca ttttttgtcg caggcaaaga agctaccgct gcctgggttg
    19501 aagaagcgac caactcaggt tgcctggtga tcgacagcag cggattgttt gctctcgaac
    19561 ccgacgtacc gctggtggtg ccggaagtaa acccgtttgt actgaccgat taccgcaacc
    19621 ggaatgtcat cgccgtaccg gacagtctga ccagccagtt gctggcggca ctgaaaccgt
    19681 taatcgatca gggcggttta tcacgtatca gcgttaccag cctgatttca gcctccgccc
    19741 agggcaaaaa agcggtcgat gcgttagcgg ggcagagtgc taaattgctc aacggcattc
    19801 cgattgacga agaagatttc ttcgggcgtc agctggcgtt caacatgctg ccgttactgc
    19861 cggatagcga aggtagcgtg cgtgaagaac gtcgtatcgt tgacgaagta cgcaaaatcc
    19921 tgcaggacga aggactgatg atttcggcaa gcgtcgtcca ggcaccggta ttctacggtc
    19981 atgcccagat ggtcaacttt gaagctctgc gtccactggc agcagaagaa gcgcgtgatg
    20041 cgtttgttca aggggaagat attgtgctct ctgaagagaa cgaattccca actcaggtag
    20101 gtgatgcttc tggtacgccg catctttctg ttggctgcgt gcgtaatgac tacggtatgc
    20161 cggagcaagt ccagttctgg tcggtggccg ataacgttcg ctttggcggc gcgctgatgg
    20221 cagtaaaaat cgccgagaaa ctggtgcagg agtatctgta ctaatgtccg accagcaaca
    20281 accgccagtt tataaaattg cgctgggcat tgagtacgac ggcagtaagt attacggctg
    20341 gcaacggcag aacgaagttc gcagtgtgca ggagaagctg gaaaaggcgc tctcccaggt
    20401 ggcgaacgaa cccatcaccg tcttctgcgc cgggcgtact gacgcagggg tacacggtac
    20461 cgggcaggtt gtgcatttcg aaacaaccgc gcagcgcaaa gacgctgcgt ggacgttggg
    20521 cgtaaatgcg aatttacctg gtgacatcgc cgtgcgttgg gttaaagctg tacctgatga
    20581 ttttcatgcc cgatttagcg ccacggctcg ccgttatcgc tacatcatct acaatcatcg
    20641 gctgcgcccg gcggtactga gtaaaggggt aacccatttt tacgaaccgc tggatgctga
    20701 acggatgcat cgggctgcgc aatgcttgct gggcgagaat gatttcacct cgttccgtgc
    20761 ggtgcagtgc cagtcccgaa ccccgtggcg caacgttatg catattaatg tcacgcgtca
    20821 cggtccttat gtggtggtag atatcaaagc gaatgccttt gtacatcata tggtcaggaa
    20881 tattgtcggc agcctgatgg aagtcggtgc ccacaaccag ccggagagct ggatagcaga
    20941 gttgctggcg gcaaaggaca gaacgctggc ggcagcaacg gcaaaagcgg aagggctgta
    21001 tctggtcgcg gtggattacc ctgaccggta tgatcttcca aaaccgccaa tgggcccgct
    21061 atttctggcg gactaacgag aataatgctc ggaacatttc aggctgtagc caacgtagtt
    21121 acagcctgaa agatgacgag tacaaggcat aggcaaatat ggacctgatt tatttcctca
    21181 tcgattttat cctgcacatt gacgtgcatc tggcagagct ggtcgcggag tacggcgtct
    21241 gggtttatgc cattttgttc ttaattttgt tctgtgaaac cggtctggtg gtaacgccgt
    21301 ttttaccggg tgattcattg ctgtttgttg ccggagcgtt ggcatcactg gaaaccaacg
    21361 atctcaacgt ccatatgatg gtggtgctga tgctgatcgc cgcgattgtg ggtgacgcgg
    21421 tcaattacac tatcgggcgg ttgttcggtg aaaagttatt cagtaatccc aattcgaaaa
    21481 ttttccgtcg cagttatctc gacaaaaccc atcagtttta tgagaaacat ggcggcaaaa
    21541 cgattattct cgcccgtttt gtgccgatcg tcagaacgtt cgccccgttt gttgcgggaa
    21601 tgggccacat gtcgtaccgt catttcgctg cttataacgt gatcggcgca ctgttgtggg
    21661 tactgctttt tacctacgca ggctatttct tcggtacaat cccgatggtt caggataacc
    21721 ttaagctgct gatcgtcggg attattgtgg tttctatttt gccgggcgtc atcgaaataa
    21781 tccgtcacaa acgcgctgcg gcacgcgccg caaaataaaa agtaactccg cggttcgacc
    21841 acttttttat ccaaagtttc gggctgttat gttttaatgt gcaacattca tggtctgttg
    21901 ggggcaaaaa tggcattatg cgtccccaaa gataaaactg gcatcgaacc aggttcagac
    21961 agaaaggtcc ctaatgagct ggattgaacg aattaaaagc aacattactc ccacccgcaa
    22021 ggcgagcatt cctgaagggg tgtggactaa gtgtgatagc tgcggtcagg ttttataccg
    22081 cgctgagctg gaacgtaatc ttgaggtctg tccgaagtgt gaccatcaca tgcgtatgac
    22141 agcgcgtaat cgcctgcata gcctgttaga tgaaggaagc cttgtggagc tgggtagcga
    22201 gcttgagccg aaagatgtgc tgaagtttcg tgactccaag aagtataaag accgtctggc
    22261 atctgcgcag aaagaaaccg gcgaaaaaga tgcgctggtg gtgatgaaag gcactctgta
    22321 tggaatgccg gttgtcgctg cggcattcga gttcgccttt atgggcggtt caatggggtc
    22381 tgttgtgggt gcgcgtttcg tgcgtgccgt tgagcaggcg ctggaagata actgcccgct
    22441 gatctgcttc tccgcctctg gtggcgcacg tatgcaggaa gcactgatgt cgctgatgca
    22501 gatggcgaaa acctctgcgg cactggcaaa aatgcaggag cgcggcttgc cgtacatctc
    22561 cgtgctgacc gacccgacga tgggcggtgt ttctgcaagt ttcgccatgc tgggcgatct
    22621 caacatcgct gaaccgaaag cgttaatcgg ctttgccggt ccgcgagtta tcgaacagac
    22681 cgttcgcgaa aaactgccgc caggattcca gcgcagtgaa ttcctgatcg agaaaggcgc
    22741 gatcgacatg atcgtccgtc gtccggaaat gcgcctgaaa ctggcgagca ttctggcgaa
    22801 gttgatgaat ctgccagcgc cgaatcctga agcgccgcgt gaaggcgtag tggtaccccc
    22861 ggtaccggat caggaacctg aggcctgata actgataagg gcagggccac tggctctgcc
    22921 cttttgctat tctcaccgta acgaatcagc ggataccatg attatcaaac gcactcctca
    22981 agccgcgtcg cctctggctt cgtggctttc ttatctggaa aacctgcaca gtaaaactat
    23041 cgatctcggc cttgagcgcg tgagccaggt cgcggcgcgt cttggcgtcc tgaaaccagc
    23101 gccatttgta tttaccgttg cgggtacgaa tggcaaaggc accacctgcc gtacgctgga
    23161 gtcgattctg atggcggcag ggtacaaagt gggcgtctac agttcgccgc atctggtgcg
    23221 ttataccgag cgcgtacgtg tgcagggcaa ggaattgccg gaatcggccc acaccgcctc
    23281 ttttgcggag attgaatcgg cacgcggtga tatttccctg acctatttcg agtacggtac
    23341 gctgtcggcg ttgtggctgt tcaaacaggc acaacttgac gtggtgattc tggaagtagg
    23401 gctgggcggt cgtctggacg caaccaatat tgtcgacgcc gatgtcgcgg tagtaaccag
    23461 tattgcgctg gatcataccg actggctggg gccagatcgc gaaagtattg gtcgcgagaa
    23521 agcaggcatc tttcgcagcg aaaaaccggc aattgtcggt gagccggaaa tgccttctac
    23581 cattgctgat gtggcgcagg aaaaaggtgc actgttacaa cgtcggggcg ttgagtggaa
    23641 ctattccgtc accgatcatg actggacgtt tagcgatgct cacggcacgc tggaaaacct
    23701 gccgttgccg cttatcccgc aaccgaatgc cgcaacggcg ctggcggcac tgcgtgccag
    23761 cgggctggag gtcagtgaaa atgccattcg cgacgggatt gccagcgcaa ttttgccggg
    23821 acgtttccag attgtgagcg agtcgccacg cgttattttt gatgtcgcgc ataatccaca
    23881 tgcggcggaa tatctcaccg ggcgtatgaa agcgctaccg aaaaacgggc gcgtgctggc
    23941 ggttatcggt atgctacatg ataaagatat tgccggaact ctggcctggt tgaaaagcgt
    24001 ggttgatgac tggtattgtg cgccactgga agggccgcgc ggtgccacgg cagaacaact
    24061 gcttgagcat ttgggtaacg gcaaatcatt tgatagcgtt gcgcaggcat gggatgccgc
    24121 aatggcggac gctaaagcgg aagacaccgt gctggtgtgt ggttctttcc acacggtcgc
    24181 acatgtcatg gaagtgattg acgcgaggag aagcggtggc aagtaagttt cagaatcggt
    24241 tagtgggcac gatcgtgctg gtggcgctgg gggtgattgt acttccaggg ctgctggacg
    24301 ggcagaaaaa acattatcag gatgagtttg cggctatccc gctggtgcca aaagcgggcg
    24361 atcgcgatga gcctgatatg atgccagccg ccacccaggc gttgccgacg cagccgccgg
    24421 aaggcgcagc ggaagaggtg cgggctggtg atgccgcagc accgtcgctc gatccgacca
    24481 ctattgcagc caataacacc gagtttgaac cggaacctgc accggtcgcc ccaccgaagc
    24541 cgaaaccggt ggagccgccg aaaccgaagg ttgaagcacc acctgcgccg aagccagaac
    24601 cgaagccggt cgtggaagaa aaagctgcac cgacgggtaa agcctatgtt gtgcaactgg
    24661 gggcgctgaa aaatgccgat aaagtgaatg agattgtcgg taagctgcgc ggtgccggtt
    24721 atcgggttta tacgtcgcca tccacgccag tgcagggtaa aattacccgt attctggttg
    24781 ggccagatgc ctcgaaagat aagctgaaag gatcgctggg tgagttgaag caactttctg
    24841 gcttaagtgg cgtggtaatg ggctatacgc cgaattaata cggtcttgcc tgatgcgacg
    24901 ctggcgcgtc ttatcaggcc tacgcagggg tagaacccta ggtcggataa ggcgtttacg
    24961 ccgcatccga cagccgttgc ctgatgcgac gctggcgcgt cttatcaggc ctacgcaggg
    25021 gtagaaccgt aggtcggata aggcattcac gccgcatccg acagccgttg cctgatgcga
    25081 cgctggtgcg tcttatcagg cctacgcagg ggtagaaccg taggtcggat aaggcgttca
    25141 cgccgcatcc gacagccgtt gcctgatgcg acgctggcgc gtcttatcag gcctacgcag
    25201 gggtagaacc gtaggtcgga taaggcgttt acgccgcatc cgacacgcat tgcacgatgc
    25261 cgcaaaggca taaaaagtcg atggcgttga atattttttc agcgccattt ttattgatgc
    25321 gcgggaagga aatccctacg caaacgtttt ctttttctgt tagaatgcgc cccgaacagg
    25381 atgacagggc gtaaaatcgt gggacacata tggtctggat tgattacgcc ataatcgcgg
    25441 tgattgcttt ttcctctctg gttagcctga tccgcggctt tgttcgtgaa gcgttatcgc
    25501 tggtgacatg ggggtgtgct ttctttgttg ccagtcatta ctacacttac ctgtcagtct
    25561 ggtttacggg ctttgaagac gaactggttc gaaatgggat tgccatcgcg gtactgttta
    25621 tcgctaccct gatcgttggt gctatcgtga acttcgtgat aggccagctg gtggagaaaa
    25681 cggggttgtc aggcaccgat cgggtgctgg gcgtctgttt cggtgcgttg cgcggtgtgt
    25741 tgattgttgc tgccattctc ttctttctcg actcctttac cggggtgtcg aaaagcgaag
    25801 actggagcaa atcacagctg atcccgcagt tcagttttat catcagatgg ttttttgatt
    25861 atctgcaaag ctcgtcaagt ttcttgccca gagcgtaagt gctctgagat gtggcttaac
    25921 gaggaaaaag acgtatgtgc ggtattgtcg gtatcgccgg tgttatgccg gttaaccagt
    25981 cgatttatga tgccttaacg gtgcttcagc atcgcggtca ggatgccgcc ggcatcatca
    26041 ccatagatgc caataactgc ttccgtttgc gtaaagcgaa cgggctggtg agcgatgtat
    26101 ttgaagctcg ccatatgcag cgtttgcagg gcaatatggg cattggtcat gtgcgttacc
    26161 ccacggctgg cagctccagc gcctctgaag cgcagccgtt ttacgttaac tccccgtatg
    26221 gcattacgct tgcccacaac ggcaatctga ccaacgctca cgagttgcgt aaaaaactgt
    26281 ttgaagaaaa acgccgccac atcaacacca cttccgactc ggaaattctg cttaatatct
    26341 tcgccagcga gctggacaac ttccgccact acccgctgga agccgacaac attttcgccg
    26401 ccatcgctgc tacaaaccgc ttaatccgcg gcgcgtatgc ctgtgtggcg atgattatcg
    26461 gccacggtat ggttgctttc cgcgatccga acggtattcg tccgctggtt ctgggaaaac
    26521 gtgatattga cgagaaccgc acagaatata tggtcgcttc cgaaagcgta gcgctcgata
    26581 cactgggctt tgatttcctg cgtgatgtcg cgccaggcga agcgatttac atcactgaag
    26641 aagggcagtt gtttacccgt caatgtgctg acaatccggt cagcaatccg tgcctgtttg
    26701 agtatgtata ctttgctcgc ccggactcgt tcatcgacaa aatttccgtt tacagcgcgc
    26761 gtgtgaatat gggcacgaaa ctgggcgaga aaattgcccg cgaatgggaa gatctggata
    26821 tcgacgtggt gatcccgatc ccggaaacct cgtgtgatat cgcgctggaa attgctcgta
    26881 ttctgggcaa accgtaccgc cagggcttcg ttaaaaaccg ctatgttggc cgcaccttca
    26941 tcatgccggg ccagcagctg cgtcgtaagt ccgtgcgtcg taaactgaac gccaaccgcg
    27001 ccgagttccg cgataaaaac gtcctgctgg tcgacgactc catcgtccgt ggcaccactt
    27061 ctgagcagat tatcgagatg gcacgcgaag ccggagcgaa gaaagtgtac ctcgcttctg
    27121 cggcaccgga aattcgcttc ccgaacgttt acggtatcga tatgccgagc gctacggaac
    27181 tgatcgctca cggtcgcgaa gtagatgaaa ttcgccagat catcggtgct gacgggttga
    27241 ttttccagga tctgaacgat ctgatcgacg ccgttcgcgc tgaaaacccg gatatccagc
    27301 agtttgaatg ctcggtgttc aacggcgtct acgtcaccaa agatgttgat cagggctacc
    27361 tcgatttcct cgatacgtta cgtaatgacg acgccaaagc agtgcaacgt cagaacgaag
    27421 tggaaaatct cgaaatgcat aacgaaggat gatgccttcg ctcagggtgc cggtctggca
    27481 ccctgacttg caactcccgc cgaaatcctg caaagtctgc ctgcaagtct gacagggcaa
    27541 ctatttatga aacgacttat tgtgggcatc agcggtgcca gcggcgcgat ttatggcgtg
    27601 cgcttattac aggttttgcg cgatgtcaca gatatcgaaa cgcatctggt gatgagccag
    27661 gcagcgcgcc agaccttatc cctcgaaacg gatttttccc tgcgcgaagt gcaggcatta
    27721 gccgatgtca cgcatgatgc gcgcgatatt gccgccagca tctcttccgg ttctttccag
    27781 acactgggga tggtgatttt accctgttca atcaaaaccc tgtccggcat tgtccatagc
    27841 tatactgatg gcttactgac ccgtgcggca gatgttgtgc tgaaagagcg tcgcccgttg
    27901 gtgctctgcg tgcgtgaaac accattgcac ttaggccatc tgcgtttaat gacgcaggca
    27961 gcagaaatcg gtgcggtggt tatgcctccc gttccggcgt tttatcatcg cccgcaatcc
    28021 cttgatgatg tgataaatca gacggttaat cgtgttcttg accagtttgc aataaccctt
    28081 cctgaagatc tctttgcccg ctggcagggc gcataactcc gctgttgccc tgtttcaggg
    28141 caattttgca accgcgatca aatcctcgac attttgtttc tgccattcaa tcgaaacgct
    28201 gcgattcaac cgctatacct gctatcttca actttaggac aataatgcaa cgtcttatta
    28261 acatatttaa cgttgaatgt tactgttgtc gtcaagatgg cataagacct gcatgaaaga
    28321 gcctgcaaac acacaacaca atacacaaca taaaaaagcc attttcactt gagggttatg
    28381 tatgaagaag tcgattctcg ctctgtcttt gttagtcggt ctctccgcag cggcttccag
    28441 ctatgcggcg ctaccggaga cggtacgtat cggaaccgat accacctatg cgccgttctc
    28501 gtcgaaagat gctaaaggtg attttgttgg ctttgatatc gatctcggta acgagatgtg
    28561 caaacggatg caggtgaaat gtacctgggt tgccagtgac tttgacgcgc tgatcccctc
    28621 actgaaagcg aaaaaaatcg acgccattat ttcgtcgctt tccattaccg ataaacgtca
    28681 gcaggagatt gccttctccg acaagctgta cgccgcagat tctcgtttga ttgccgccaa
    28741 aggttcgccg attcagccaa cgctggattc actgaaaggt aaacatgtgg gggtgctgca
    28801 gggatcaacc caggaagctt acgctaacga gacctggcgt agcaaaggcg tggatgtggt
    28861 ggcctatgcc aaccaggatt tggtctattc cgatctggct gcaggacgtc tggatgccgc
    28921 actgcaggat gaagtggcgg ccagcgaagg tttcctcaag caacccgccg gtaaagagtt
    28981 cgcttttgct ggctcatcag tgaaagacaa aaaatacttc ggtgatggca ccggtgtagg
    29041 gctacgtaaa gatgatactg aactgacggc tgcgtttaat aaggcgcttg gagagctgcg
    29101 tcaggacggc acctacgaca agatggcgaa aaagtatttc gactttaatg tctacggtga
    29161 ctgatacgtc gcagggaagc tgtgcctgat ggaatgatca tcatggtgca cgccaggttt
    29221 gttgcactat cgtggtgcat tgaaatgcat acttaagcat ttttaatgaa aaataatacg
    29281 tctaacgggg cgggatattt tgccttgatg gtcaattt
//
LOCUS       DAZUCE010000035        29169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZUCE010000035 DAZUCE010000000
VERSION     DAZUCE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.035"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            42..638
                     /locus_tag="ACHY03_001362"
     CDS             42..638
                     /locus_tag="ACHY03_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3904617.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            725..1450
                     /locus_tag="ACHY03_001363"
     CDS             725..1450
                     /locus_tag="ACHY03_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFS3904618.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVAPFSQASFGVTGLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1492..4092
                     /locus_tag="ACHY03_001364"
     CDS             1492..4092
                     /locus_tag="ACHY03_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFS3904619.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSGNSSAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGLATSSLEIPFVDIGENSAAACLTRKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVRFDSSDQFLDLTIPQAYVLKNYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNNNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQNGYKIYETTVPPGEFVIDDLSPSGFGSELVITIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDNLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTDYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNSGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINAPGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSATTVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQD
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTLGSSNTLLL
                     NNLTCQVANH"
     gene            4106..4654
                     /locus_tag="ACHY03_001365"
     CDS             4106..4654
                     /locus_tag="ACHY03_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3904620.1"
                     /translation="MKRILLTSALIGLGLPAVEAATTLNVDFTATVLATTCTITIVQD
                     GGPVVTGSNGEYSLTIPDVGLDKVVTGDVAAQANFKLLASDCSNGYSKIFTTLTGATV
                     SGKLIVNEASSGATGVGMGIKRRDTADSAFFTPNNTDKFEWTADEKTSGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4669..5280
                     /locus_tag="ACHY03_001366"
     CDS             4669..5280
                     /locus_tag="ACHY03_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3904621.1"
                     /translation="MKLKVIATLIATVAVGASVSNNFTYASTTSASLTVNSNLTMGTC
                     SAKILDTSDTEINTVAFNNVYISELAAKSKVQPFKIRFSDCAGLPGKKAQLVLSPKTG
                     VNCAGGLSNTAGFANTISSGNGGSSRAAVEVWTTTTPGGNGSQQFSCVQRNVINVDLS
                     NASTTQPYDFDLSARMIIADGWTVNDVLPGDFLSPTTFTITYQ"
     gene            5301..5891
                     /locus_tag="ACHY03_001367"
     CDS             5301..5891
                     /locus_tag="ACHY03_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFS3904622.1"
                     /translation="MLCRHHKIVRFLGLAAALITPFGYAGQDVDLTAKIVASTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     SLAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            5903..7015
                     /locus_tag="ACHY03_001368"
     CDS             5903..7015
                     /locus_tag="ACHY03_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3904623.1"
                     /translation="MQRKGNRLLIQLCSAILLFFTTSWYALANECYIERNAEGPYEMV
                     ITSKPFSIPTRMVTAPEEIAFATWDVHIDLRGDKIGCKSLGSDTSSVHFVNYADADLL
                     STYTTTNGFALLKTTVPGVVYSVELVCVSCGAAEELDLYLPAQSGADNHTGNAGNKWA
                     YEYSDDSWYLRFRFFTTPEFKPQTGVSEGYAKPGRIASWYIGNTDQPWINFYVKDTSV
                     HFYVDEPTCQTVALAQDLGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRAEYCYASKI
                     TVKLKAANKPSDATLVGKTTGSASGVAVKVHSTYENSKVLLKADGSNSVEYNFAIWVN
                     NLLNLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            7151..7945
                     /gene="panB"
                     /locus_tag="ACHY03_001369"
     CDS             7151..7945
                     /gene="panB"
                     /locus_tag="ACHY03_001369"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFS3904624.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7957..8808
                     /gene="panC"
                     /locus_tag="ACHY03_001370"
     CDS             7957..8808
                     /gene="panC"
                     /locus_tag="ACHY03_001370"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFS3904625.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8904..9113)
                     /locus_tag="ACHY03_001371"
     CDS             complement(8904..9113)
                     /locus_tag="ACHY03_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904626.1"
                     /translation="MEIKTRRETRQTLAQWFEEKGFQKGYQEELQKVRQEFAQRFLSK
                     GMSREDVAEVTTLPLTEIDKLINSN"
     gene            complement(9182..10054)
                     /gene="rpnC"
                     /locus_tag="ACHY03_001372"
     CDS             complement(9182..10054)
                     /gene="rpnC"
                     /locus_tag="ACHY03_001372"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFS3904627.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGETTPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEERQEFVLRLLNKGMSREDVAE
                     MANLPLAEIDKLIN"
     gene            10328..10708
                     /gene="panD"
                     /locus_tag="ACHY03_001373"
     CDS             10328..10708
                     /gene="panD"
                     /locus_tag="ACHY03_001373"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFS3904628.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10712..11941)
                     /gene="yadE"
                     /locus_tag="ACHY03_001374"
     CDS             complement(10712..11941)
                     /gene="yadE"
                     /locus_tag="ACHY03_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFS3904629.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGGLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12005..12445)
                     /gene="yadI"
                     /locus_tag="ACHY03_001375"
     CDS             complement(12005..12445)
                     /gene="yadI"
                     /locus_tag="ACHY03_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS3904630.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12550..13320)
                     /gene="yadH"
                     /locus_tag="ACHY03_001376"
     CDS             complement(12550..13320)
                     /gene="yadH"
                     /locus_tag="ACHY03_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3904631.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13317..14243)
                     /gene="yadG"
                     /locus_tag="ACHY03_001377"
     CDS             complement(13317..14243)
                     /gene="yadG"
                     /locus_tag="ACHY03_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3904632.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14352..15014
                     /gene="can"
                     /locus_tag="ACHY03_001378"
     CDS             14352..15014
                     /gene="can"
                     /locus_tag="ACHY03_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFS3904633.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15055..15591)
                     /gene="hpt"
                     /locus_tag="ACHY03_001379"
     CDS             complement(15055..15591)
                     /gene="hpt"
                     /locus_tag="ACHY03_001379"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFS3904634.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15797..18187
                     /gene="gcd"
                     /locus_tag="ACHY03_001380"
     CDS             15797..18187
                     /gene="gcd"
                     /locus_tag="ACHY03_001380"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFS3904635.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVITASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            18226..18324
                     /locus_tag="ACHY03_001381"
                     /pseudo
     CDS             18226..18324
                     /locus_tag="ACHY03_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18389..19939)
                     /gene="cueO"
                     /locus_tag="ACHY03_001382"
     CDS             complement(18389..19939)
                     /gene="cueO"
                     /locus_tag="ACHY03_001382"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFS3904636.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20105..20452
                     /gene="yacC"
                     /locus_tag="ACHY03_001383"
     CDS             20105..20452
                     /gene="yacC"
                     /locus_tag="ACHY03_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFS3904637.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20558..21424
                     /gene="speE"
                     /locus_tag="ACHY03_001384"
     CDS             20558..21424
                     /gene="speE"
                     /locus_tag="ACHY03_001384"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFS3904638.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21440..22234
                     /gene="speD"
                     /locus_tag="ACHY03_001385"
     CDS             21440..22234
                     /gene="speD"
                     /locus_tag="ACHY03_001385"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFS3904639.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22272..22634)
                     /gene="yacL"
                     /locus_tag="ACHY03_001386"
     CDS             complement(22272..22634)
                     /gene="yacL"
                     /locus_tag="ACHY03_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFS3904640.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22809..25406)
                     /gene="acnB"
                     /locus_tag="ACHY03_001387"
     CDS             complement(22809..25406)
                     /gene="acnB"
                     /locus_tag="ACHY03_001387"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFS3904641.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVYNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25761..27614
                     /gene="yacH"
                     /locus_tag="ACHY03_001388"
     CDS             25761..27614
                     /gene="yacH"
                     /locus_tag="ACHY03_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFS3904642.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPLSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTVPVIPSN
                     TVSAANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMTA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSPSPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27685..29109)
                     /gene="lpdA"
                     /locus_tag="ACHY03_001389"
     CDS             complement(27685..29109)
                     /gene="lpdA"
                     /locus_tag="ACHY03_001389"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFS3904643.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
BASE COUNT         7319 a         7674 c         7099 g         7077 t
ORIGIN      
        1 cgcaatatta atttcatata atttttgtaa ggatgcatgt aatgaaaaaa gcacttctcg
       61 cagccgctct ggttatggct tctggttccg ccctggctgt agatggtggt catatcgact
      121 ttaacggtat ggtacagtcc ggtacctgta aagtgggtgt ggtagatact ggtatgcaca
      181 gcgttaccac tgatggcgtg gttaccctgg atactgcgaa tgttactgat acttttgctg
      241 aagttagcgc aactgctgtc ggtttactgc cgaaagagtt catgatttct gttgagtgtg
      301 atccaggtgc tccgaagaat gctgagttaa ctatgggttc tgcaagctac gcgaacacca
      361 gcggtaccct gaataacaat atgaacatca ctgttaacgg tattgcaccg gctcagaacg
      421 taaacattgc agttcataac atgaaaaaca aagctggcgc tgctgaaatt aagcaggtcc
      481 atatgaacaa ctcttctgaa gttcaggaac tgacattaga cgcagaaggt aaaggccagt
      541 acgtatttaa cgcatcttac gttaaagcac cgaacagccc ggctgtaact gctggtcatg
      601 taaccactaa cgcgctgtac accgttgctt ataagtaatt gatggtgtgt tgagcataag
      661 gtttgccagc aggcaaacct tattttcgcg aatttaatct ttctacagac agcaaggagt
      721 gcttatgttt cccttggtta aaaaaacggt tagtgcatta tttgtgagta ctctgttagc
      781 cagtgcacca gcattcgctg acattattat ttccggaaca cgtatcattt ataacgctga
      841 taaaaaagat gttaacgttc gcctggaaaa taaagggaat cgtcccttac tgattcaaaa
      901 ctggttggat acgggcgatg acaatgccga tcccagccag atcaaagtgc cgtttaccgc
      961 gaccccgccc gtttcacgcg tagagccaaa acgcggtcaa accgtaaaag tgatgtacac
     1021 cggtgcaaca gccctgccga aagaccgtga aagcgtctac tggtttaacg tgctggaagt
     1081 gccgcctaag ccgaaagatg ctgaggctga caaaaacttg ttacagctgg ctttccgtac
     1141 gcgcatcaaa ctcttttatc gcccgagcgg tttacaggga gaaccggctg aagcaccggc
     1201 gaaaatcacc tggaaattaa acaacgctca gttacaggca aataacccaa cgccatatta
     1261 cgtgtccttt aatgaggtca agctggaaag cggcggcaaa acctacaacg tcaattccag
     1321 catggtcgcg ccgttcagcc aggcaagctt tggcgtaacc ggtctgccag gaagcgtttc
     1381 atccggaaaa gtggtcttta aagcgatcaa cgactttggc ggcaacattg atggttccgc
     1441 aacattctaa aaaataaacg gattcttttt aacatcgcca ggaatgcaac cttgtaccag
     1501 tttactcatc aaaaaagccg tatcccgaaa aaaacgctac ttgcggcctg ttgcgccctt
     1561 ttttacagcg gcaacagttc tgcggcggac accgtggaat atgacagttc cttcttaatg
     1621 ggaactggcg catcaacgat tgatgttaaa cgttatgctc aaggcaaccc gacaccgccg
     1681 ggtctctata atgtccgcgt gtttgtaaac ggtctggcta cgtccagctt agaaattccg
     1741 tttgtggata ttggcgaaaa cagtgcggcg gcctgtctta cccgcaaaaa cctggcgcag
     1801 ctacacatta agcaaccaga acagcctgtc actttactcg ccagggaagg tgaagaagag
     1861 gattgtctgg atctggcaaa gtcatacgaa aaggcggatg tgcgctttga cagcagtgac
     1921 cagtttctcg atctgactat ccctcaagct tatgttctga aaaactacgg tggttatgtc
     1981 gacccctcac tgtgggagtc agggattaac gctgccacac tggcatatac cctgaacgcg
     2041 tatcacacaa gttcagataa caacaatagt gacagcgtct atggcgcgtt taactcaggt
     2101 atcaacttag gtgcctggca cttccgggcg cgtggtaact ataactggac aacagataac
     2161 ggcagcgatt tcgatttcca ggatcgttac ttacaacgtg acattccggc aatccggtca
     2221 caaataatca tgggtgacgc ctacaccacc ggtgaaacgt ttgactctgt caacgtccgt
     2281 ggtgttcgtc tatacagcga tagccgtatg ctgccttccg cgctggccag ttacgctccg
     2341 accatccgcg gtgtggcaaa ctccaacgcc aaagtcacgg tgacgcagaa cggttataag
     2401 atctatgaaa ccaccgttcc gcccggtgag tttgttatag acgacctcag cccctccggc
     2461 tttggtagcg aactggtcat taccattgaa gaagcggatg gttccaaacg cacctttacg
     2521 cagcccttct cctccgttgt gcaaatgcag cggcctggcg taggtcgttg ggatttcagt
     2581 gcgggtaaag tcattgatga caacctgcgc tcagaaccga atatggggca agcctcttat
     2641 tactatggtc tgaataacct cttcacgggg tataccggca ttcagttcac cgataacaac
     2701 tatcttgccg gactgttagg tgtgggtatt aacaccagta tcggtgcttt tgcggtagac
     2761 gttacccact cccgtgctga gattccggat gataaaacct atcaggggca aagttatcgc
     2821 gtgacctgga acaaactttt ccaggatacc ggaacatcat ttaacctcgc ggcataccgc
     2881 tattccaccc aggattacct gggcctgcat gatgcgttag tcctcattga cgacgccaaa
     2941 catttgtctg ccgatgaaga caaaaacacc atgcagacgt actcacgtat gaaaaaccag
     3001 tttaccgtga gtattaacca gccattgaat atcgcctatg aagattacgg ttcgctgttt
     3061 atttccggta gctggacaga ctactgggcg gctaacaata gccgcactga atataatgtt
     3121 ggttacagta agagcgtttc ctggggcagc ttcagcgtca acctgcaacg tagctggaac
     3181 gaagatggcg agaaagatga tgcaatgtac gtcagcgtta gcgtacctat tgagaatatt
     3241 ttaggtggca aacgtaagtc ttctggtttc cgcaatttaa atactcagct caataccgat
     3301 ttcgatggtt cacatcagtt gaatgttaac agttccggta acactgaaaa caatctggtg
     3361 aactacagtg tcaacgcagg ttatagcctc gataaaaact ccggcgattt agcctctgtt
     3421 ggtggttatc tcaactatga atcagggtta ggcggtattt ccgcttcggc ctcggccact
     3481 tcggataaca gccaacagta ctccatctcc accgatggag gctttgtatt acacagtggt
     3541 ggtttaacgt tcactaacaa cagtttcagc agtaacgaca cgctggtgtt aatcaacgcc
     3601 ccaggtgcta aaggcgcacg aatcaataac agtaataacg aaatcgatcg ctggggatat
     3661 gccgtaacgt cctctgtcag cccctaccgt gaaaaccggg taggtctgaa tattgaaacg
     3721 ctggaaaacg atgttgaact gaaaagtacc agcgccacca ccgtaccacg cagcggctcc
     3781 gttgttttga cccgtttcga aactgacgag gggcgttctg ccgtgctgaa tattaccgct
     3841 gccaatggca aatccattcc gtttgctgcg gaggtttatc aggatgaagt gatgatcggc
     3901 agcatgggtc agggtggtca ggcatttgtg cgcggcatta acgacagcgg ggaattaatc
     3961 gttcgctggt atgaaaacaa ccaaaccatt gactgtaagt tgcactacca gttcccggcc
     4021 caaccacaaa cactgggaag cagcaatacc ttattactta acaatcttac ctgtcaggta
     4081 gcaaatcact aatatggaaa attctatgaa aaggatacta ctaacatccg cgttaatagg
     4141 tctgggttta ccggcggttg aagctgcaac aactcttaat gtcgatttta cggccaccgt
     4201 attagccacc acctgtacga taactatagt acaagatggt ggcccagtag tgacgggtag
     4261 taacggtgaa tacagcctga caattcctga tgttggcctg gataaagttg tcactggaga
     4321 tgttgcagct caagccaact ttaaattact ggcttccgat tgcagcaatg gttactcaaa
     4381 gatttttacc acacttacag gtgcaactgt ttcaggtaag ttgattgtca atgaagcaag
     4441 ctctggcgct acgggtgtgg gtatggggat taagcgtaga gataccgcag actcagcttt
     4501 ctttacgcca aataatactg acaaatttga atggaccgcc gatgagaaaa ctagcggtgt
     4561 gcctctgacc gtagcactgc gcgaaacaac ggcgggagca ggacgcaccg gtgcgtttca
     4621 ggcaaaagcc acctttaact tcacctacga ataacaggga cagccaccat gaaattaaaa
     4681 gtcatcgcta cactgattgc tactgttgcc gtgggtgcca gcgttagcaa caatttcact
     4741 tatgcgagta cgacatccgc ctctttaacc gtaaacagta acctgactat gggtacctgt
     4801 agtgcgaaga tactggatac cagtgatacc gagatcaata ccgtcgcctt taacaatgtt
     4861 tatatttctg aactggcagc caaaagcaag gtacaacctt ttaaaatccg cttcagcgat
     4921 tgtgccggcc ttccaggaaa gaaagctcaa ctcgtattgt cgcccaaaac tggtgtcaat
     4981 tgcgctggtg gcctgtctaa cactgccggc tttgcaaata caatttcctc cggcaatggt
     5041 ggttcaagca gagccgcagt cgaagtatgg acgacaacga cacctggggg aaatggcagc
     5101 cagcagttca gttgtgtaca acgaaacgtc atcaatgtcg atctttctaa tgccagcaca
     5161 acacagcctt atgatttcga tttaagcgcg cgaatgatta tcgctgacgg gtggacagtc
     5221 aatgacgtat taccgggtga tttcctctct cccacaactt tcactatcac ttatcagtaa
     5281 tagcggataa ggatattaga atgctatgca ggcatcataa aatcgtacgt tttttggggc
     5341 tggcagcagc ccttattacg cctttcggct atgcaggtca ggatgtggat ctcaccgcga
     5401 agattgtggc cagcacctgt caggtggaag tcagtaataa tggcgtggta gatctcggca
     5461 cagtgacgct ggactatttt gctgacaatg tgaccccaac gacggattac gcgggtggca
     5521 aaacgtttaa tgttaatgtg gtctcctgcg acaacattca aacgacgcaa tcacaaatga
     5581 aattagattt tcagccgcag gcgggatcgc ttgcgcaggc aaataatcaa atatttagta
     5641 acgaatatga acagcaggct accggggcaa aaaacgtcgg cattgtgata ttttcggcac
     5701 agccgaatca gcagacattt aatgtacgcg ggacggatgg ttcctcaaca gcgatttatt
     5761 cagtcgcgcc tggcaatgct gttccctcaa cctggacctt ttattcccgt atgcagcgag
     5821 tgaataatgc ccttcctccc gaatcaggga tggtgagaag tcaggttatt gttaacgttt
     5881 cttacgaata ataaggataa ttatgcaaag gaaaggcaat agactgttaa ttcagttatg
     5941 cagtgcgata ctgctatttt ttaccacatc ctggtatgca ttggcgaatg aatgttatat
     6001 agaacgcaat gctgaagggc cttatgagat ggtgataacc tcaaagccct tcagtatacc
     6061 gacacgaatg gtgacagcac cggaagaaat agcctttgcg acctgggatg tgcatattga
     6121 ccttcggggg gataagattg gctgtaaatc cttagggagt gacacaagct cagtacattt
     6181 tgttaactat gcagatgctg atttgttatc aacctatacc accactaatg gctttgcctt
     6241 attgaaaaca accgttcctg gtgttgttta ttctgtggaa cttgtttgtg taagttgtgg
     6301 tgccgcagag gaactggatt tgtatcttcc agcacagagt ggcgcagata accatacggg
     6361 gaatgctggt aacaaatggg cctatgagta cagcgatgat agttggtatt tgaggtttcg
     6421 ttttttcacg actcctgaat tcaaacccca aactggggtt tcagaaggat acgccaaacc
     6481 aggtagaatc gcctcctggt atataggtaa tactgaccag ccatggataa atttctacgt
     6541 taaagatacc tccgtacatt tttatgtgga cgaaccgacc tgtcaaacag ttgccctggc
     6601 tcaagacctg ggtaatgtca gcggcaacca ggtaacgctt gggaacagct atgtttcgga
     6661 agtgaaaaat gggcttacac gggaaattcc tttttctatc cgcgctgaat actgttacgc
     6721 cagtaaaatt acggttaagt tgaaagcggc aaataaaccc agcgacgcca cactagtagg
     6781 taaaacgact ggctctgctt caggcgtggc tgtaaaagtg cattcgactt acgaaaatag
     6841 caaagtctta ttaaaagcgg acggtagcaa ctccgttgag tacaacttcg caatctgggt
     6901 aaacaacctg ctgaatttgc cctttacggc acagttggta ccggatggta gcggtaatgc
     6961 tgtcggtgtt ggaacctttt caggtaacgc gaccttctcc tttacctacg aataagtaaa
     7021 acgtcattcc ttcctcatcg caccagagtt ctgctctggt gcgaacattt tccccacact
     7081 aaaaaccatt gcccccctga tttcgactgt tagaatgcga tcgatctgac tttagccatc
     7141 aggatacgtt atgaaaccga ccaccattgc ttcattgcaa aaatgcaaac aggaaaaaaa
     7201 acgtttcgcg accatcaccg cttacgacta tagcttcgcc aaactctttg ccgatgaagg
     7261 acttaatgtc atgctggtag gcgactcgct gggcatgacg gttcaggggc acgactccac
     7321 cctgccagtt accgttgctg atatcgccta ccacactgcc gccgtacgtc gtggcgcgcc
     7381 aaactgcctg ttgttggctg acctgccgtt tatggcgtat gccacgccgg aacaagcttt
     7441 tgaaaacgcc gcaacggtta tgcgtgccgg tgctaacatg gtcaaaattg aaggcggtga
     7501 gtggctggtc gaaaccgtaa aaatgctgac cgaacgtgcc gttcctgtat gtggtcactt
     7561 aggtttaacc ccacagtcag tgaatatttt cggtggctac aaagttcagg ggcgcggcga
     7621 tgaagcgagc gatcgactgc ttagcgatgc attagcctta gaagccgctg gtgcacagtt
     7681 gctggtgctg gaatgcgtgc cggttgaact ggcaaaacgt attaccgaag cgctggcgat
     7741 cccggttatc ggtattggcg caggcaacgt taccgatggg cagatcctcg tgatgcacga
     7801 tgcgttcggc attaccggcg gtcatatccc taaatttgcc aaaaatttcc tcgctgaaac
     7861 gggcgacatc cgcgcggctg tgcggcagta tatggctgaa gtggagtccg gcgtttatcc
     7921 gggcgaagaa cacagtttcc attaaggagt gacgttgtgt taattatcga aaccctgcca
     7981 ctgctgcgcc agcaaattcg ccgcctgcgt atggaaggca agcgcgtggc gctggtacct
     8041 accatgggca acctgcatga tggccacatg aagctggtcg acgaagccaa agcccgcgcc
     8101 gatgtggtcg tcgtcagtat tttcgttaac ccgatgcagt tcgaccgccc agaagacctg
     8161 gctcgttacc cacggacctt gcaggaagac tgcgaaaaac tgaacaagcg caaagtggat
     8221 ctggttttcg cgccctcggt aaaagagatc tacccgaacg gtactgaaac ccatacttac
     8281 gttgacgttc cagggctttc gaccatgctg gaaggcgcca gccgtccggg gcattttcgc
     8341 ggcgtgtcga ctatcgtcag caagctgttt aacctggtcc agccggacat cgcctgcttc
     8401 ggtgaaaagg actttcagca actggcgctg atccgcaaaa tggttgctga tatgggcttt
     8461 gatattgaga ttgttggcgt cccgattatg cgcgccaaag atggccttgc gctcagttcc
     8521 cgtaacggtt atctgacggc ggaacaacgt aaaattgcgc ccggtctgta caaagtttta
     8581 agttcaattg ccgacaaatt gcaggctggc gaacgggatc tcgatgaaat tattgctatt
     8641 gccgggcaag aactgaatga aaaaggcttc cgcgccgatg atattcagat tcgcgatgcc
     8701 gacacgttgc tggaagtctc tgaaaccagc aaacgagctg tgattctggt agccgcctgg
     8761 cttggcgatg cccgcctgat cgacaacaag atggtcgaac tggcgtaata cttaactggc
     8821 gctacggctg atggcgccag ttattaattt gccctacgtc atggcgatga cgtctgtaag
     8881 tgattattca tatcgctggc aatttagtta gagttaatta gcttatcaat ctctgtaaga
     8941 ggtaaagttg tcacctctgc aacgtcttcc cgagacatcc ctttacttag aaaacgctgg
     9001 gcgaattcct gtcttacctt ctggagttcc tcttgatatc ccttctgaaa tcccttctct
     9061 tcaaaccact gcgccaacgt ctgcctggtt tctctccgtg tctttatttc cataggttcc
     9121 cttaattatc tggtcagtta acaataacaa ttatctggct ataactgatg atgcagacaa
     9181 cttagttaat cagcttatca atctcggcaa ggggtaaatt tgccatctct gcaacgtctt
     9241 cccgagacat tcctttattc agaagacgca ggacgaattc ttgcctttcc tcctgtcttc
     9301 ccctctcttc aaaccactgc gccagcgtca tcatagactc ccctcccgtt tccctgtctc
     9361 tcagcacacc ataaaacaaa tccgcttgtt cagtatgacc gcgttgcagc atatagtttt
     9421 gcatggcaac taactgactt ccgctagtgt acccttcgtc aatcagcgtg accagttgct
     9481 ccagcaataa cattaagtcg cgctggcgaa tatgcttttg cagtagttcg agaatcgcaa
     9541 tccgccgatg ttgcatgatt tcgtcatccg gtgtgatagt gatatccacc agcggaaaag
     9601 gactgttata gacgcgtcgc gccagctccg gcgagtaaaa catatcaaac cagcacattg
     9661 agagcggata aggtgtggtc tcgccctgat aaaacagtat cggcaccacc agcggcagct
     9721 tatcgtgatc cgcctccaga tgccggtgca tggcagcaat agaataacgc atcatgcgaa
     9781 aggccatttt cttatccggc ttgctttggt gttcaatcac aacatgcaga taaccgggat
     9841 tgccctgcat ttgcacggaa tagagcacgt ccgtgctgtg tcctttcagg ctctcttcaa
     9901 tgaaactccc cgactctaaa tgaagcgtgt tgaggtcaca aagttctcgt aattccactg
     9961 gcaaatgtat ctccagaaag tcgcgagccg tctccgcgtg cattaaaaat tgtttaaaca
    10021 ctgcatcatg cggtgtggta ctcggtgcat ccatcttccc tgccttccgt gttgtcttgc
    10081 gtgaaggcag tatccagtca aggtgtacca tcgtcagcca cctctttagt gtttaccgag
    10141 cagcgttcag agaaatttca attggttaca gccgttacat ggcacgcttc ggcgcggtac
    10201 gcaaactgct gatgcaaatg ccgtcagatg agtagacact aaacaaaaat cgggcaatac
    10261 tgcgtgagaa tttcctcaaa gcaggcaccg cctgcttcgt taacgacagg gtagaaaggt
    10321 agaagttatg attcgcacga tgctgcaggg caaactccac cgcgtgaaag tgactcatgc
    10381 ggacctgcac tatgaaggtt cttgcgccat tgaccaggat tttcttgacg cagccggtat
    10441 tctcgaaaac gaagccattg atatctggaa tgtcaccaac ggcaagcgtt tctccactta
    10501 tgccatcgcg gcagaacgcg gttcgagaat tatttctgtt aacggtgcgg cggcccactg
    10561 cgccagtgtc ggcgatattg tcatcatcgc cagcttcgtt accatgccag atgaagaagc
    10621 tcgcacctgg cgacccaacg tcgcctattt tgaaggcgac aatgaaatga aacgtaccgc
    10681 gaaagcgatt ccggtacagg ttgcttgatt gttatccctg cggctggtta ctcaccagcc
    10741 gcgacatcgt ctccagcgaa tccgttctta agatataaag tcgttttagt aacaacggat
    10801 tatcccccgg ttttactttg cctttcatgg ttgtcaccgc caggtgaaat ccggcatcgt
    10861 ttgctgcctt cacggctttg tcattaaatc cgccaaacgg atacgaaaga taccagacat
    10921 gcggattaaa ttgcgccaga gcgcggcgtg aacgtgcaaa atcaaacaga atattgtgct
    10981 cactacgact cagtaatatg gggcggcgat aaccatctac ccgatgcaaa aaatgggtat
    11041 gtgactggaa atcaaataca tcgcgaattt cgttaagctc agaaacgctc ataaattgca
    11101 gcgattttgg ttcccacttc tgcgggtgac gtttgatgcg cgaggtaaca ataaacgccg
    11161 tcgccttcat gccatattgt ttcaacacag gatacgcata gcggctcacc gacttgaggc
    11221 catcatcaaa ggtaatcacc accgctcgcg cagggagatt gatcttattc ttcacgtaac
    11281 cttccagctg cgccatgctc agtgtcgcgt atcccctgtc gcgcagccag gccatctggt
    11341 tattgaaagc gcgtaccgag gtggtcgtcg aagtatggcg aaaacgggtg ttttcttcgt
    11401 cgcgcagaat atggtgataa gttagcaccg gaaggccatt atcgggttgg gcatccagcg
    11461 cgctgatata ggccagtcga tcgccaatac ggacctgata ccaggtttga tttaacctgt
    11521 ctttcagttt atgcaaaatc gggtagcgca aattgtccgc caatccccca aatggcgcac
    11581 tgcccacact cggcgcgtta tagaccggcg tatctttcca ggtaactaag ttctgattac
    11641 tcagcggctt gttgaggtca cccaaaccgt cttcaacttt ttgtcgcccc tgaaccggct
    11701 cgagatggcc tttatcgata aaccctttac caaagccaaa attaaatgcg taataacttg
    11761 cggcagtggg ctccacggca atgatttgtc cggcccgaat attccccacg gtcaccatct
    11821 tgtcgccaat ttgagcccag accgcagcat tttcgatggt ttgcatataa cgggcaggta
    11881 acgcggcact gacacttgcg gtaaacagca tcagcaggag aataacagct tgtttgtaca
    11941 tgaaaaaata accgagggtg aaacaacgct tttattgtag caaaagcgcc atcagaatcg
    12001 cgggttaata ctcatacaaa ttatgtttga ggacgaaagg cggatttttt tgttgttggt
    12061 gccaaagact actcacctcc ggcgcaccca gttcgacaat acgctcgcga aactctgaac
    12121 tggtcatggt ttcacggcaa gccatcatct gttcaattaa cggtaacgtg acaccagaaa
    12181 tcacttcgca acgggagtgt ttgtggctta ataatgaagc cacgcgatac ggtggcgctc
    12241 cggctatatc ggttaagaag atgacaccct caccagagtc cgcttcatgc agagcatcgc
    12301 acatcatgcg gctgagcata ttagagctta acccacgcca gaaattcacg gcccggcact
    12361 gaagaagcgc cccatgtttt ttctccagcg catccagtat ctcttgcgcc cggtcatcgt
    12421 gacaggtaat tacccaacct aacatcgcct tcctccttag ccgaaaatca gtctaccctg
    12481 accattgttg ataaagggtg atagccgtca aaactcctct cccccaaatc cagaggagag
    12541 gaaatagcct tagctacgca aaccacgtcc acgttggatc agcgaccaac agatcaaata
    12601 aaacgccaca ataaagacca ccagtacgcc aaaggtagtg accagcggaa catcattgat
    12661 accgaggaag ccgtagcgga aaccactgat catataaacg attgggttca ggtgcgacag
    12721 cccttgccag aacggcggca acaaagtcag tgagtaaaag accccaccca aatacgtgag
    12781 tggcgttaac acaaaggttg gcaccaggct gatgtcatcg aacgttttgg caaacacacc
    12841 gttcagcaaa cccgcaaggg agaacaacac cgccgtgagc accagggtta aggcaacgaa
    12901 tacccacgaa tgcacctgaa acggcacaaa aaacagtgaa attgccgtca ccagaatgcc
    12961 aacaaacaga ccacgcgcca cgccaccgcc gacatatccg gcaataatga cgtgagtcgg
    13021 aaccggcgct accagcagct cttcaatatt acgctggaac ttggcaccaa aaaatgatga
    13081 cgcgacgttg gcgtaggcgt tggtgatcac cgacatcatg atcagccccg gtacgatgaa
    13141 ctgcatgtag ctgaagccat gcatatcgcc aatacgcgaa ccaatcaggt taccgaaaat
    13201 aataaagtaa agggtcatgg tgatgactgg cggcaccagc gtctgcaccc agatacgcat
    13261 aaagcgatgg atctctttcg cccagatgct ttttagcgcc acccagtaaa gatgcatcat
    13321 gcgcgatctc cttgtttttc attaaccagt gaaacaaaca gctcttccag acggttagct
    13381 ttgttacgca tacttaatac ctgaatgccc tgctcactta actgcgtaaa tacgctgttg
    13441 atcccctgct cacgcagcac ttcaacttcc agcgtcgcgg tatcgaccag tcgatactga
    13501 tagccatcga gcttcggtaa cggacttttc ggtgcgagat cgagaataaa ggtttccgat
    13561 ttcagcttcg ccagcagcgc cttcatcgag gtattttcca ccagctcacc gtgttgaata
    13621 atgccgatat tgcggcacag catttctgct tcttccaggt agtgtgtggt gagaatgatg
    13681 gtggtgcctt tgtcgtttaa atccttcaaa aagccccaca ttgagcggcg aagttcaata
    13741 tccacgcctg cagtcggctc gtcgagaatc agtagtttag gttcatgcat taacgcacgg
    13801 gcaatcatta aacggcgctt catcccgcca gataacatgc gcgcacgttc gttgcgtttt
    13861 ccccataaat cgagctgctt aagatatttt tcgctgcgga tgtacgcctc tttgcgctcc
    13921 acaccgtagt accctgcctg attcaccaca atttgctgca cggtttcaaa cgggttgaag
    13981 ttaaattcct gcggcaccag tcccaactga cgtttagcgt tcacgacatc tttctcgaga
    14041 tcgtaaccaa atacgctgac ccgcccggag gttttattta ccagagagct gataataccg
    14101 atagtggtcg atttcccggc cccgttcggc ccgagaagcg cataaaaatc acccgcttcg
    14161 acctgcaaat ctattccacg aagcgcctga acgccgcctg gataggtttt tttaagctgt
    14221 tgaagttcca gtgcaatggt cataaatttt tacttacctt acgttcttac actttatctg
    14281 tggtttaaat cgtcccggag ttgccctata ttagccaaac gtaattattt ggttacaggt
    14341 cgttaacctc catgaaagac atagatacac tcatcagcaa caatgcacta tggtcaaaaa
    14401 tgctggtgga agaggatccc gggttttttg agaaactggc acaagcgcaa aaaccgcgct
    14461 ttctatggat tggatgttcc gacagtcgcg ttcctgcaga acgtttaacc ggtcttgagc
    14521 cgggcgaact ctttgttcac cgtaatgttg ctaacctggt cattcacacc gacctgaact
    14581 gcctttccgt ggttcagtat gcagtggatg tactcgaagt tgaacacatt attatctgtg
    14641 gccactacgg ttgcggcggc gtacaagccg cagttgaaaa cccggaactg gggcttatca
    14701 acaactggct gctgcatatc cgcgatatct ggttcaaaca tagctcattg ctcggcgaaa
    14761 tgccgcaaga gcgccgtctg gataccttgt gtgaactgaa cgtcatggaa caggtgtata
    14821 acctgggcca ctccaccatt atgcaatcag cgtggaaacg cgggcagaaa gttaccattc
    14881 acggctgggc ctatggcatt cacgacggct tgctgcgtga tctggatgtt actgccacca
    14941 accgcgaaac ccttgagcaa cgttaccgtc acgggatttc caacctcaag ctgaaacacg
    15001 ccaaccacaa ataaaaatgc catgccggat gcaacacatc cggcaacttc acacttactc
    15061 gtccagcaga atcactttgc cgatatacgg cagatgacga taacgctggg cgtaatcaat
    15121 gccgtaaccc accacaaact catccgggat cgagaaaccg ataaattcaa ccgggacgtt
    15181 cacttcacga cgggacggtt tatccagcag cgtacaaatc gccaacgact tcggttcgcg
    15241 caggcttaag atctcacgca ctttcgacag tgtattcccc gagtcgatga tatcttcaac
    15301 aatcagcacg tccttgccac ggatatcttc atccagatct ttgaggattt tcacatcacg
    15361 ggtggtggac atgccgctac cgtagctgga ggcggtcata aagtcgactt catgagatac
    15421 ctgaacttca cggcacaggt ccgccataaa cataaatgag ccacgcagca gacccaccag
    15481 caccatatcg ctgccgctgt ctttgtaacg ctcagtaatc tgacgaccca gttcggcgat
    15541 acgcgcttta atctccgctt cggggatcat tacttctaca gtatgtttca tatctctaac
    15601 catatgattt aaaaacaaat cacttcgcac gggcaggttt tggcaccgca acaaagcgca
    15661 agacacgcag tataccagta aaactattca ttcgcggaat ctgttaataa agctcgaatt
    15721 gtgatgacga tcacacatgt taaaacctat aattaattgc tactaaaata ttaatgaatt
    15781 gaaatggtgt ctctttatgg caattaacaa tacaggctcg cgacgattac tcgtcacgct
    15841 aacagccctt tttgcagcgc tttgcgggct gtatctactc attggcggag gctggctggt
    15901 cgcgattggc ggctcctggt actaccctat cgctggcctt gtgatgctcg gcgtcgcctg
    15961 gatgctgtgg cgcagtaaac gtgccgcgct ttggctatac gccgccctgc tgctcggcac
    16021 catgatttgg ggcgtctggg aagttggttt cgacttctgg gcgctgactc cgcgcagcga
    16081 cattctggtc ttcttcggca tctggctgat cctgccgttt gtctggcgtc gcctggtcat
    16141 tactgccagc ggcgcagttg ccgcactggt ggtggcactg ctgattagcg gtagtatcct
    16201 gacctgggcc ggatttaacg atccgcagga aatcaacggc accttaagcg ccgatgccac
    16261 acctgcggaa gctatctcac ccgttgccga tcaggactgg cctgcctatg gtcgcaatca
    16321 ggaaggtcaa cgcttttcac ctctgaaaca aattaacgcc gataacgtcc ataatctgaa
    16381 agaagcctgg gtgttccgta ctggcgatgt gaagcagccg aacgatccgg gcgaaatcac
    16441 caatgaagtg acgccgatta aagtgggcga caccctttac ctgtgtaccg ctcaccagcg
    16501 tctgtttgcg cttgatgccg ccagcggtaa agagaaatgg cattacgatc ctgagctgaa
    16561 aaccaacgag tctttccagc acgtaacctg ccgtggcgtc tcttatcatg aagccaaagc
    16621 agaaacggct tcgccggaag tgatggcgga ttgcccgcgt cgtatcattc ttccggtcaa
    16681 tgatggtcga ctgattgcga ttaacgctga aaacggcaaa ctgtgcgaaa ccttcgccaa
    16741 taaaggcgtg ctcaatctgc aaagcaatat gccggacacc aaaccgggtc tgtatgaacc
    16801 gacttcgcca ccgattatca ccgataaaac catcgtgatg gcaggttcag ttaccgataa
    16861 cttctcaacc cgcgaaacgt ctggcgtgat ccgtggtttt gatgtcaaca ccggggagct
    16921 gctgtgggct tttgatcccg gcgcgaaaga tccgaacgca atcccgtctg acgaacacac
    16981 ctttaccttt aactcgccaa actcgtgggc accagcggcc tatgacgcga agctggatct
    17041 ggtgtatctg ccgatgggcg tgaccacgcc ggatatctgg ggcggtaacc gcacaccgga
    17101 acaggaacgt tatgccagct cgattctggc gctgaatgcc acaaccggga aattggcgtg
    17161 gagctaccag accgttcacc acgacctgtg ggatatggat cttccggcac agccgacgct
    17221 ggcggacatc accgttaatg gtcagaaagt gccagttatt tacgctccgg cgaaaaccgg
    17281 caacattttt gtgctcgatc gtcgtaatgg cgaactggtg gttccggcac cggaaaaacc
    17341 ggttccccaa ggtgctgcca aaggcgatta cgttacccct actcaaccat tctcggaact
    17401 gagcttccgt ccgacgaaag atttgagcgg tgcggatatg tggggagcca ccatgtttga
    17461 ccaactggtg tgccgcgtga tgttccacca gatgcgctat gaaggcattt tcacgccgcc
    17521 atctgaacag ggcacgctgg tcttcccggg taacctggga atgtttgaat ggggcgggat
    17581 ttccgttgat ccaaatcgtg aagtggcaat tgccaaccca atggcactgc cgtttgtttc
    17641 gaaactgatc ccacgcggtc cgggtaaccc gatggagcag ccgaaagatg ccaaaggcac
    17701 gggtacggaa tccggcattc agccacagta cggtgtaccg tatggtgtca cgctcaaccc
    17761 gttcctctca ccatttggtc tgccatgtaa acagccagca tggggttata tctcggcgct
    17821 ggatctgaaa accaacgaag tcgtgtggaa gaaacgtatt ggtacgccgc aggacagcat
    17881 gccgttcccg atgcctgtac cggtgccgtt caatatgggt atgccgatgc tgggcgggcc
    17941 aatctccacg gcgggtaacg tgctgtttat cgccgctacg gcagataact acctgcgcgc
    18001 ttacaacatg agcaacggtg aaaaactgtg gcagggtcgt ttaccagcgg gtggtcaggc
    18061 gacgccaatg acctatgaag tgaatggtaa gcagtatgtg gtgatctccg caggcggtca
    18121 cggttcattt ggtacgaaga tgggcgacta tattgtggct tatgcgctgc cggatgatgt
    18181 gaagtaagac ttgctcagat tgctgacaac gtgcgcgttg ttcatgccgg atgcggcgtg
    18241 aacgccttat ccagcctaca aaattgtgca aaatcaatgg attgcacagg tagcgtaggc
    18301 ctgataagcg tagcgcatca ggcagatttg cgtttgtcat cagtttaatg cccggagaga
    18361 tccgggcata tttccgaata cggtcttttt ataccgtaaa ccctaacatc atccccgtat
    18421 cttcatgctc cagcagatgg cagtgcgcca tataagcatg ttctttcggt gcgtcgtgat
    18481 taaacttcac cagcacttca ctgacattgc cttctacctt aacggtatct ttccagcccg
    18541 cgcgatgagc cgctggcggt ttgccatttt ctgacaagat acggaactgc gtgccgtgaa
    18601 tatggaacgg atgcagcatc atgtcgccca cgccagagat aacccaacgt tcgtactgcc
    18661 ctttcgccgc cgcaaacatc ggcttattca tatcaaacgc ctgaccgttg attttattgg
    18721 catggtggaa atcgaacttc ccgccgtggt tcatatgatt catattgccg tgccccatat
    18781 ggcccatcat ctggctgtga tccattccgg ccatcgcctg atcgccatat ttctccatta
    18841 gcatctgcat ccccatcata tcgagcatcg ggtccataga aagttgcagc ttgcgcaccg
    18901 tcagcccttc cagcgaaggt aacgcaggca ggctacttaa tgtgtctggc aaagcaccgg
    18961 aggcactaat agcaatcggc tgaatccgca ttaccggatg cggcttgtca aacggtgcaa
    19021 tcgccatccc catctggctg accggcagcg tcaccaggtc aaagggtttg ttgtcgttaa
    19081 cctccaccag cacttcaaaa cgctcgccca tcagcaccgg cagctcgttc accttcaccg
    19141 gttcaggtag cagaccaccg tcgctggcaa tcacataaag cgggcgattg tcgctggtgg
    19201 cgaaattgag cgagcgggca ttacagccat tgagcaaacg caggcgcagc caaccacgcg
    19261 gggcagcgtg ttgcgggtag attgcaccgt tggtcagcaa cgtatcgcca aaccagccca
    19321 cggcggcggt catcacatcc agttgataat caatctgccc gtcggcgcta aatttcttat
    19381 cctgaacgat caccggaaca tcatcgatac cccactgttt tggcagcatt aatttcagga
    19441 tctcgtcatc ttcaatcacc accagaccag ccagccccat cgccacctgt cgcccggtct
    19501 tgccatgttg atgtggatgg aaccagcagg tagcggcagg ttgatcaacg ttcaacgtca
    19561 ccgagcgctt gccacctggc ggaataattc cctgtgggcc gccgtcgact tcacccggta
    19621 cttccagccc gtgccagtgc aacgtcgtct cttccgtcag ttggttatag atatcaaccg
    19681 ttaccgcttt gccgcgctgt aatttcaccg ccggccccag cagattgccg ttatagcccc
    19741 aggtagttgc agttttctcg ccaaaggtag actgacctgc gccaatagtt aactgaatgc
    19801 gattacgggc atcggtcgtg agcaaatcag ggatcggtaa cgttgggcgt tccgccgcaa
    19861 agactgcacg gctccacagt ggcaaggctg aagccacacc cagtgcgacg gaatatttca
    19921 agaaatcacg acgttgcata gttatttcct tatttttaag caggcgaaac aaaatcaaac
    19981 gttgagcata gtccttcccc ttacgggaag gtcaagccgc agacacaatt taatcgacaa
    20041 taatggtcgt cgcctcgctc gcagtgtgct aacgtttatc ttctttaagc ccctggtaga
    20101 agcaatgaag acgtttttca gaacagtgtt attcggcagc ctgatggccg tttgcgcaaa
    20161 cagttacgcg ctcagcgagt ctgaagccga agatatggcc gatttaacgg cagtttttgt
    20221 ctttctgaag aacgattgtg gttaccagaa cttacctaac gggcaaattc gtcgcgcact
    20281 ggtctttttc gcccagcaaa accagtggga tctcagtaat tacgacacct tcgacatgaa
    20341 agctctcggt gaagacagct accgcgatct cagcggcatt ggcattcctg tcgctaaaaa
    20401 atgcaaagcc ctggcccgcg attccttaag cctgcttgcc tacgtcaaat aatccctgat
    20461 acctttttgt agaaatattg accgtgcatc cgcggtcaat gttagctatt atgttgcgcc
    20521 ctttttttac gggtgttaac aaaggaggta tcaacccatg gccgaaaaaa aacagtggca
    20581 tgaaacgcta cacgaccagt ttgggcagta ctttgcggta gataacgttc tgtatcatga
    20641 aaagaccgat caccaggatc tgatcatttt tgagaacgct gcatttggtc gcgtaatggc
    20701 gctggatggc gtagtacaaa ccaccgagcg cgacgagttt atctatcatg agatgatgac
    20761 ccatgttccg ctactggccc acggtcacgc gaaacatgtg ctgattatcg gcggcggcga
    20821 cggtgccatg ctgcgtgaag taacccgaca taaaaacgtt gagtcaatca cgatggtgga
    20881 aatcgatgcg ggtgtcgtat cgttctgccg tcagtatcta cccaaccata acgccggtag
    20941 ctacgacgat ccgcgcttta agctggtgat cgatgatggc gtcaatttcg ttaatcaaac
    21001 cagccagaca tttgatgtca ttatctccga ctgcaccgat cctatcggtc ccggcgaaag
    21061 ccttttcact tcggcatttt atgaaggctg caaacgttgc ctgaatcctg gcggtatctt
    21121 cgtcgcacaa aacggcgtct gctttttaca gcaggaagaa gccatcgaca gccatcgcaa
    21181 actcagccat tacttcagcg acgttggctt ttatcaggcg gcgatcccga cctattacgg
    21241 cggtatcatg acttttgcat gggcgacaga taacgacgcc ttacgccatc tctcaaccga
    21301 aattattcag gcacgttttc tcgcctctgg cctgaaatgc cgttattaca atccggcagt
    21361 ccatacggca gcttttgcct taccccagta tctgcaagac gcactggctt cacagccgtc
    21421 ctaaggagaa gataagaaat tgaaaaaact gaaactgcat ggctttaata atctgaccaa
    21481 aagtctgagt ttttgtattt acgatatctg ctacgccaaa actgccgaag agcgcgacgg
    21541 ttatattgct tatatcgatg aactctataa tgccaaccgt ctgaccgaaa tcctgtcaga
    21601 aacctgttcc attatcgggg ctaatattct taacatcgcc cgccaggatt acgaaccaca
    21661 gggtgccagc gtcactattc tggtgagtga agaaccggtt gacccgaaac tcatcgacaa
    21721 aacagaacac cccggcccac tgccagaaac ggtcgttgcc catctcgata aaagtcatat
    21781 ttgcgtacat acctacccgg aaagtcatcc tgaaggcggt ttatgtacct tccgcgccga
    21841 tattgaagtc tctacctgcg gcgtgatttc tccgctgaag gcgctgaatt acctgatcca
    21901 ccagcttgag tccgatatcg taaccattga ttatcgcgtg cgcggtttta cccgcgacat
    21961 taacggtatg aagcacttta tcgaccatga gattaattcg attcagaact ttatgtctga
    22021 cgatatgaag gcgctgtatg acatggtgga tgtaaacgtc tatcaggaaa atatcttcca
    22081 taccaagatg ttgcttaaag agttcgacct taagcactac atgttccaca ccaaaccgga
    22141 agatttaacc gacagcgagc gccaggaaat taccgctgcg ctgtggaaag aaatgcgcga
    22201 gatttattac gggcgcaata tgccagctgt ttaacggctc tggcggagct cccaggctcc
    22261 gccagattta tttacttctg ctgcacgaaa ttgcggtaag ccgccaccac ctgcagaaaa
    22321 tcctcaacgc cgcatagcga caggctttct tcgtcgtagt agttcatccc ctcttccatt
    22381 tcatcgccag cgaattccag ttgattggcg cgaaccatca cctcttcacc gtccatccac
    22441 agggtgtatt catgccctgc ccgttgccag gaccgttcgc tacctttcag tgcgtgcgct
    22501 gcttgttcca cttcatcaag caaggccagg ttttctttca cctcttcatt aaaccagtgc
    22561 ccgaccactt catgccccat ggacatacgc acctttacca ctccggtaat atcgcgcaga
    22621 aattcgtaat ccatagtgtg ttcctctgct cccggcaatg cgccgaaatc gcattgcgcc
    22681 attgatgtaa ttatcgcagc cttaccggag aaaaacagcg gaacggcgat ggtttagaaa
    22741 taaaaaatgc ccgggaatct cattcaccgg gcattgtgtc gtttatgcgc agcgcgtgcg
    22801 ctgacttttt aaactgcagt ctggaaaatc acaccatcgg ctttttcagt gtactgagaa
    22861 agctggttga agttcaggta acggtaagta tcaacggcag ttttatccac ctgcgccacg
    22921 taggtctggt actcttccgg cgtcggtagt ttgccaatca gcgccgcaac agcagccagt
    22981 tccgcagaag ccaggaagac attcgcgcca gtacccagac ggttcgggaa gttacgggtc
    23041 gaggtggaaa ccaccgtcgc accgtctgcc acacgcgcct ggttgcccat acacagggaa
    23101 cagccaggga tctcgatacg cgcaccactc ttaccgaaga cgctgtagta gccttcttcg
    23161 gtcaactgtg cggcgtccat acgggttggc ggtgccaccc acaggcgggt cggcaactgg
    23221 cctttatgcg catccagcag tttacccgca gcacggaagt gaccgatgtt ggtcatgcag
    23281 gaaccgataa acacttcgtc gatcttctca ccctgtaccg cagacagcgg acgcgcgtca
    23341 tccgggtcgt tcggagcaca caggattggc tctttaatat ccgccagatc gatgtcgatc
    23401 actgccgcgt attccgcatc tgcatcggct tccagcagct caggattcgc cagccatttt
    23461 tccatgccct gaatacgacg ttccagggtg cgacgatcgc cgtaaccttc cgcgatcatc
    23521 cacttcagca ggacgatgtt agagttcagg tattcgatga tcggttcttt gttcagcttg
    23581 atggtacaac cggcagcaga acgctcggcg gacgcatcgg tcagctcaaa ggcctgctca
    23641 actttcagat ccggcagacc ttcaatttcc agaatgcgac ctgagaagat gtttttcttg
    23701 cctttcttct caacggtcag cagaccttgt ttgatcgcat acagcggaat ggcgtgtacc
    23761 agatcgcgca gggtgatgcc cggctgcatt ttgcctttga agcgcaccag aacggattcc
    23821 ggcatatcca gcggcattac gcctgttgcg gctgcaaatg ccaccagacc agaacccgcc
    23881 gggaaagaga taccgatcgg gaaacgggta tgggagtcac caccggtacc gacggtatcc
    23941 ggcagcagca tacggttcag ccaggagtgg attacgccgt cacccggacg cagcgacaca
    24001 ccgccacggt tcataatgaa gtccggcagc gtgtggtgcg tgttcacgtc aactggcttc
    24061 ggatacgccg cggtgtggca gaaagactgc atcaccaggt cagccgagaa gcccaggcac
    24121 gccaggtctt tcagttcatc acgggtcatc gggcctgtgg tgtcctgaga gcctacagaa
    24181 gtcattttcg gttcacagta cgcgcccgga cgaatgcctt tcacgccaca ggcacggcct
    24241 accattttct gtgccagcga gaagccgcga tcgctctcag cgacatcttt cgcctgacgg
    24301 aacacatcac tgtgcggcag accaagtgct tcacgcgctt tggtggtcag gccacgcccg
    24361 ataatcagcg gaatacggcc gccagcacgc acttcatcaa tcagcacgtc ggttttcagt
    24421 tcgaaggtcg ccagcagttc gcctgtttcg tggttacgca cttcaccttt gtacgggtaa
    24481 acgtcaatca cgtcgcccat gttcaggtta tagacgtcga cttcgattgg cagtgcaccc
    24541 gcgtcttcca tcgtgttaaa gaagattggt gcaattttac cgccgaggca caaaccaccg
    24601 ccgcgtttgt tcggcacatg tggaatatca tcgcccataa accacagaac ggagttggtg
    24661 gcggatttac gcgatgaacc cgtacccaca acgtcaccga cgtacgccag cgggaaacct
    24721 ttctgttgca gagcttcaat ctgtttgatc gggccaacaa cacctggctg gtctggctca
    24781 ataccttcac gggcgttttt cagcatcgcc agcgcgtgca gtgggatatc cgggcgcgac
    24841 cacgcatccg gtgccggaga gaggtcatcg gtgttggttt cgccagtgac tttgaagacg
    24901 gtaacggtca gtttttcagc cagcgccggg cgattcagga accattcggc atccgcccag
    24961 gactgcatta cctgcttcgc atattcgttg cctgctttgg ctttctcttc tacgtcatag
    25021 aagttatcga acatcagcag cgtgtgagaa agtgctttgg cagcgatcgg tgccagtttg
    25081 gcatcatcca gcgcgtcgat cagcggatga atgttgtaac caccctgcat ggtacccagc
    25141 agttcgatgg ctttttccgg agtcagcaga ggggatttgg cttcgccttt agcgatagca
    25201 gccaggaagc ctgctttgac ataggcggct tcatcgacgc ctgggggaac acggttggtt
    25261 aatagatcta acaggaattc ttcttcgccc gcgggcgggt ttttcagcag ctctacaagt
    25321 gcggccattt ggtttgcatc caggggtttg ggcgcaatcc cctcagcggc acgctcagct
    25381 acgtgcttac ggtattcttc tagcacgacg gttctcctcg ctctcattgt catagtgcgg
    25441 caggcgattc tcttcacgct cctgtgagac agcagtttgt agggtaaatg cccagtaccg
    25501 caacgggcag aatagcagga ttttgacgat gtgttaatct gtttacaaaa aagcaacata
    25561 aaaaagtggc tgaatcgtta aggatggtct agagattgtt tccctccgta aaaacttcgt
    25621 taattcgcat agtgataatc acctttctca acgaatccca agattagtca aaatttaaac
    25681 taccgcctct ttatactcag atttacagca cctgcgggtg gcagttcgcc gaccattgcg
    25741 atttccttga gatccgaatt atgaaaatga ctttgccgtt taaaccccat gtgctggcgc
    25801 taatttgcag tgccgggctt tgtgccgcct ctgccgggct atatataaaa agccgcacag
    25861 tggaagcgcc tgtggaaccg ctatcgacac aactggctgt gtctgacgct gccgcggtta
    25921 cgcttcctgc aacggtttcc gcgcctcccg taacacccgc cgtcgttaaa tccgcattca
    25981 gcactgcaca aatcgatcaa tgggttgcac ctgtcgcgct ataccccgac tctctgcttt
    26041 cacaagtgtt aatggcatca acctatccgg caaacgttgc tcaagcagtg caatggtcac
    26101 acgataatcc acttaaacaa ggcgatgctg ctattcaggc ggtatctgac cagccgtggg
    26161 acgctagcgt caaatcactg gtggcctttc cacaattgat ggcgttgatg ggcgaaaacc
    26221 cgcaatgggt gcaaaacctg ggcgatgctt ttctggcgca gccgcaggac gtgatggact
    26281 cggtacagcg attgcggcaa ctggcgcaac aaaccggttc gctgaagtca tcaaccgaac
    26341 agaaagttat taccacaacg aagaaagctg taccggtaaa acagacagtc acggttcccg
    26401 tcataccatc caataccgtt tcagctgcca accctgtcat tacagagcct gctacaaccg
    26461 tcatttccat tgagcccgcc aatcctgatg tggtctatat tcccaactac aacccaaccg
    26521 tggtttacgg gaactgggcc aatactgcgt atccgccggt ttatctgcca ccaccagccg
    26581 gagaaccgtt tgttgacagc tttgtacgcg gattcggcta tagcatgggc gttgctacca
    26641 cgtacgcact attcagcagc atcgactggg acgacgacga tcatgaccat catcatcatg
    26701 acgatgatga ttatcatcac cacgatggcg gtcatcgtga cggtaatggc tggcaacaca
    26761 acggcgacaa catcaatatc gacgtcaata atttcaaccg tatcaccggt gagcatctta
    26821 ctgataagaa tatggcatgg cggcacaatc caaactaccg taatggtgtg ccctatcatg
    26881 atcaggatat ggcaaagcgg tttcatcaaa ccgatgtcaa cggcggaatg actgccacgc
    26941 agctacctgc tccaacacgc gacagccagc gtcaggcggc agcaagtcag tttcagcaac
    27001 gaacacacgc cgcccccgtc attacacgag atacccaacg tcaggcagcg gcacagcggt
    27061 ttaatgaagc tgaacactat gggagctatg acgacttccg cgacttcagc cgtcgccaac
    27121 cactgaccca gcaacaaaag gacgccgctc gtcagcgtta tcagtcacct tctcctgagc
    27181 agcgccaggc agttcgcgag agaatgcaga ctaacccgca gatccagcag cgaagagagg
    27241 cagcgcgtga gcgcattcag cccgcctcgc ctgagcagcg ccaggcagtc cgcgagaaaa
    27301 tgcagactaa cccacagatc cagcagcgaa gagacgcagc gcgtgagcgt attcagtcag
    27361 cctcgcctga gcagcgccag gtgtttaagg aaaaagtaca gcagcgccca ctgaaccaac
    27421 agcaacgtga taacgcccgc cagcgtgttc aatcagcatc acctgaacaa cgtcaggttt
    27481 ttcgggagaa agttcaggag agccgcccac aacgtctaaa cgacagtaac catactgcca
    27541 ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgagcgc ggagcaaggc
    27601 gactggaaag gtaaattaca ggcgtaaaaa aagcggcgtg gttagccgct tttttaattg
    27661 ccagatgtcc cggcaaacga aaaattactt cttcttcgct ttcgggttcg gcaggtcagt
    27721 aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag tcgggtgcgc
    27781 gtggatggtc agtgcgatgt cttcagcatc acaacccatt tcgattgcca ggccgatttc
    27841 acccagcagc tcgccgccgt tagtaccgac aatcgcacca ccgatcacac ggtgagattc
    27901 tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag cacgaccaga
    27961 agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg cttctttctc
    28021 agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga taactttcgg
    28081 atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt cgtgaacacc
    28141 tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt gcggtacgtt
    28201 ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg tcaacttcca caccagcttt
    28261 gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca cggcgtcgta
    28321 acgctgcggt tcagcgggtg cttttttgcc ttccatcgtc acataaatgc cgtcttcttt
    28381 cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc tgatacgctt
    28441 ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga acatttcaac
    28501 cacgtcaatc tgtgaaccca gcgcgtggta aacggtgccc atttccagac cgatgatacc
    28561 gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt cagtggagtc
    28621 ccagatacgc ggatcttcat gcggaataaa cggcagttga atcgggcgag aacccgctgc
    28681 aatgatcgcg ttgtcgaagt tgatcacggt tttaccgttc tcaccttcaa cttccagggt
    28741 gttagccccg gtaaatttac ccagaccgtt gaccactttg actttgcggc ctttcgccat
    28801 accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac gaatcttgtc
    28861 aatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt tggcttcttc
    28921 gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt tcaggcaaac
    28981 accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat cagcgcaacg
    29041 gaaggcagcg gagtaacctg cggggcctgc cccaagtacc acgacctgag ttttgatttc
    29101 agtactcatc atgacctcta tatatttatc tccggcgggt ctgacgtttt ttttatgacg
    29161 cccattatc
//
LOCUS       DAZUCE010000036        28890 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZUCE010000036 DAZUCE010000000
VERSION     DAZUCE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.036"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            82..1035
                     /locus_tag="ACHY03_001390"
     CDS             82..1035
                     /locus_tag="ACHY03_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3904644.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFS
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1076..2071)
                     /gene="pgl"
                     /locus_tag="ACHY03_001391"
     CDS             complement(1076..2071)
                     /gene="pgl"
                     /locus_tag="ACHY03_001391"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFS3904645.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEWAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNIDHRGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2226..3044
                     /gene="ybhA"
                     /locus_tag="ACHY03_001392"
     CDS             2226..3044
                     /gene="ybhA"
                     /locus_tag="ACHY03_001392"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFS3904646.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3045..4103)
                     /gene="modC"
                     /locus_tag="ACHY03_001393"
     CDS             complement(3045..4103)
                     /gene="modC"
                     /locus_tag="ACHY03_001393"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFS3904647.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRLLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KNMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4106..4795)
                     /gene="modB"
                     /locus_tag="ACHY03_001394"
     CDS             complement(4106..4795)
                     /gene="modB"
                     /locus_tag="ACHY03_001394"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFS3904648.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4795..5568)
                     /gene="modA"
                     /locus_tag="ACHY03_001395"
     CDS             complement(4795..5568)
                     /gene="modA"
                     /locus_tag="ACHY03_001395"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3904649.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5735..5884)
                     /gene="acrZ"
                     /locus_tag="ACHY03_001396"
     CDS             complement(5735..5884)
                     /gene="acrZ"
                     /locus_tag="ACHY03_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFS3904650.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6013..6801
                     /gene="modE"
                     /locus_tag="ACHY03_001397"
     CDS             6013..6801
                     /gene="modE"
                     /locus_tag="ACHY03_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFS3904651.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6869..8341
                     /gene="modF"
                     /locus_tag="ACHY03_001398"
     CDS             6869..8341
                     /gene="modF"
                     /locus_tag="ACHY03_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFS3904652.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPRLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8603..9619
                     /gene="galE"
                     /locus_tag="ACHY03_001399"
     CDS             8603..9619
                     /gene="galE"
                     /locus_tag="ACHY03_001399"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS3904653.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9629..10675
                     /gene="galT"
                     /locus_tag="ACHY03_001400"
     CDS             9629..10675
                     /gene="galT"
                     /locus_tag="ACHY03_001400"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFS3904654.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTVELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHSAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10679..11827
                     /gene="galK"
                     /locus_tag="ACHY03_001401"
     CDS             10679..11827
                     /gene="galK"
                     /locus_tag="ACHY03_001401"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFS3904655.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11821..12861
                     /gene="galM"
                     /locus_tag="ACHY03_001402"
     CDS             11821..12861
                     /gene="galM"
                     /locus_tag="ACHY03_001402"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFS3904656.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDCETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALTSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLHPGEE
                     YSSLTEYQFIAE"
     gene            13064..13816
                     /gene="gpmA"
                     /locus_tag="ACHY03_001403"
     CDS             13064..13816
                     /gene="gpmA"
                     /locus_tag="ACHY03_001403"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFS3904657.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13982..15034)
                     /gene="aroG"
                     /locus_tag="ACHY03_001404"
     CDS             complement(13982..15034)
                     /gene="aroG"
                     /locus_tag="ACHY03_001404"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFS3904658.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15350..15730
                     /locus_tag="ACHY03_001405"
     CDS             15350..15730
                     /locus_tag="ACHY03_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFS3904659.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15844..16785
                     /gene="zitB"
                     /locus_tag="ACHY03_001406"
     CDS             15844..16785
                     /gene="zitB"
                     /locus_tag="ACHY03_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFS3904660.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16782..17501)
                     /gene="pnuC"
                     /locus_tag="ACHY03_001407"
     CDS             complement(16782..17501)
                     /gene="pnuC"
                     /locus_tag="ACHY03_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFS3904661.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALTWLAVCVIAIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17539..18582)
                     /gene="nadA"
                     /locus_tag="ACHY03_001408"
     CDS             complement(17539..18582)
                     /gene="nadA"
                     /locus_tag="ACHY03_001408"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFS3904662.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKYPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQKQY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18860..18935)
                     /locus_tag="ACHY03_001409"
     tRNA            complement(18860..18935)
                     /locus_tag="ACHY03_001409"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18900..18902),aa:Lys,seq:ttt)
     gene            complement(18969..19044)
                     /locus_tag="ACHY03_001410"
     tRNA            complement(18969..19044)
                     /locus_tag="ACHY03_001410"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19009..19011),aa:Lys,seq:ttt)
     gene            complement(19191..19266)
                     /locus_tag="ACHY03_001411"
     tRNA            complement(19191..19266)
                     /locus_tag="ACHY03_001411"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19231..19233),aa:Lys,seq:ttt)
     gene            complement(19270..19345)
                     /locus_tag="ACHY03_001412"
     tRNA            complement(19270..19345)
                     /locus_tag="ACHY03_001412"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19310..19312),aa:Val,seq:tac)
     gene            complement(19397..19472)
                     /locus_tag="ACHY03_001413"
     tRNA            complement(19397..19472)
                     /locus_tag="ACHY03_001413"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19437..19439),aa:Lys,seq:ttt)
     gene            complement(19475..19550)
                     /locus_tag="ACHY03_001414"
     tRNA            complement(19475..19550)
                     /locus_tag="ACHY03_001414"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19515..19517),aa:Val,seq:tac)
     gene            complement(19586..19661)
                     /locus_tag="ACHY03_001415"
     tRNA            complement(19586..19661)
                     /locus_tag="ACHY03_001415"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19626..19628),aa:Lys,seq:ttt)
     gene            complement(19826..20617)
                     /gene="cpoB"
                     /locus_tag="ACHY03_001416"
     CDS             complement(19826..20617)
                     /gene="cpoB"
                     /locus_tag="ACHY03_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFS3904663.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20627..21148)
                     /gene="pal"
                     /locus_tag="ACHY03_001417"
     CDS             complement(20627..21148)
                     /gene="pal"
                     /locus_tag="ACHY03_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFS3904664.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21183..22475)
                     /gene="tolB"
                     /locus_tag="ACHY03_001418"
     CDS             complement(21183..22475)
                     /gene="tolB"
                     /locus_tag="ACHY03_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFS3904665.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22608..23873)
                     /gene="tolA"
                     /locus_tag="ACHY03_001419"
     CDS             complement(22608..23873)
                     /gene="tolA"
                     /locus_tag="ACHY03_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFS3904666.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEDAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(23938..24366)
                     /gene="tolR"
                     /locus_tag="ACHY03_001420"
     CDS             complement(23938..24366)
                     /gene="tolR"
                     /locus_tag="ACHY03_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFS3904667.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24370..25062)
                     /gene="tolQ"
                     /locus_tag="ACHY03_001421"
     CDS             complement(24370..25062)
                     /gene="tolQ"
                     /locus_tag="ACHY03_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFS3904668.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25059..25463)
                     /gene="ybgC"
                     /locus_tag="ACHY03_001422"
     CDS             complement(25059..25463)
                     /gene="ybgC"
                     /locus_tag="ACHY03_001422"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFS3904669.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25613..25906)
                     /gene="ybgE"
                     /locus_tag="ACHY03_001423"
     CDS             complement(25613..25906)
                     /gene="ybgE"
                     /locus_tag="ACHY03_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFS3904670.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(25906..26019)
                     /gene="cydX"
                     /locus_tag="ACHY03_001424"
     CDS             complement(25906..26019)
                     /gene="cydX"
                     /locus_tag="ACHY03_001424"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFS3904671.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(26034..27173)
                     /gene="cydB"
                     /locus_tag="ACHY03_001425"
     CDS             complement(26034..27173)
                     /gene="cydB"
                     /locus_tag="ACHY03_001425"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFS3904672.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27189..28757)
                     /gene="cydA"
                     /locus_tag="ACHY03_001426"
     CDS             complement(27189..28757)
                     /gene="cydA"
                     /locus_tag="ACHY03_001426"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFS3904673.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7197 a         7660 c         7129 g         6904 t
ORIGIN      
        1 ttaatttaag ttggctaaat gcagttaaaa tttttaacgg ccagccaccc aaaatcaacg
       61 cttccatttt ccggagtaat aatgaagcat gaattatcaa gtatgaaggc atttgtcata
      121 ctggcagagt ccagttcatt taacaatgcc gctaaattac tcaacattac gcaacctgca
      181 ttaacgcgaa gaataaaaaa gatggaggaa gatttacata ttcaactatt tgagcgtaca
      241 actcgcaagg ttacgttaac aaaagcagga aaaaggttgc tcccggaggc gcgggaatta
      301 ataaagaaat tcgatgaaac gctttttaat attcgtgata tgaatgctta tcatcgtggt
      361 atggtgacgt tagcgtgtat tccgaccgca gtattttatt ttttaccgct ggcaatcggt
      421 aaatttaatg agctgtatcc caatattaaa gtgcggattc tggaacaagg cacgaataat
      481 tgcatggaat cggtgctatg taacgaatca gactttggca taaacatgaa caacgtcaca
      541 aattcatcca ttgattttag cccactggtc aacgagccat tcgtgctggc ctgtcggcgt
      601 gaccatccat tggccaaaaa gcagcttgta gaatggcagg agttagtggg ttacaaaatg
      661 attggcgtac gctcctccag cggcaaccga ctgttgatag aacaacagct agccgacaag
      721 ccctggaagc tggactggtt ttacgaagtg cgtcatcttt cgacgtcatt aggactggtg
      781 gaggcaggac tggggatttc agcgcttcct ggtctggcaa tgccccatgc gccctattct
      841 tcgattattg gtatcccact ggtggaaccc gttatacgtc ggacattggg gattattcgc
      901 cgcaaagatg ccgtgctttc tccggcagct gaacgctttt ttgccttgct gattaatttg
      961 tggactgacg ataaagacaa tttgtggacc aatatcgtcg aacgccagag acatgcgtta
     1021 caggagatag gttaatacac cggcgcagga gagactgcgc cgggtaaatc agcggttagt
     1081 gtgcgttaac taccacccac atcggtccct gcccgaccgc atagcggcct ttttcatgca
     1141 gtagcccctg ctcaccaaca atttcgtata ccgagatatg gtgcgatttt tgcccggcgg
     1201 caatcagata cttaccacgg tgatcgatat taaagccgcg cggctgggtt tcagttggct
     1261 ggaagccctc tttactcaac acgctgccat cttccgaaac gctgaagaca gtaatcaggc
     1321 tggcggtacg gtcacaggca tataagtgac gaccatccgg ggtaatatga atatcagccg
     1381 cccaacgggt gtcggagaag ttttccggca tcatatccag cgtctggaca cattcgatat
     1441 tgccgtgcgg atctttcagt tcccagacat ccactgagct gtttaactca ttgacgcaat
     1501 acgcatattg ttcgtttgga tggaatacca tatgacgcgg gccggcccat tcaacggtag
     1561 tcacttccgc agggtcctgc gccaccagat gaccatcatc gctgaccgta aacaggcaaa
     1621 tgcgatcctg ctttaatgcc ggaacccaca gcgtacggtt gtccggtgag atattggcgg
     1681 aatggcaacc gtccagcccc tcgaccacat cgacgacgcc cactggcagg ccatcttcca
     1741 gacgcgttac gctcacgtta cccgcattgt aagaacctac aaagacaaac tgcccctggt
     1801 gatcggtgga aatatgcgtc ggactacccg gcagcgcaga ctctgcggca aaggtcagtg
     1861 cgccatcgtc cggggcgata cgatacgcca ggacgcgaaa ctcagggcga acaccaacat
     1921 agagataacg tttgtccggg ctgaccacca tcggctgcac ctgccccggc acatcgacaa
     1981 cctgtgtcag cgtcagtgcg ccttcatgat tcaggttcca gacgtgaatt tgctggctct
     2041 cagggctggc gatataaact gtttgcttca tgaatgctcc tttgcattta gcaactgact
     2101 gtaacagcta aaattagtcg cttttggcgg tgaatgctga attaaaacgc agaattttgt
     2161 ccgatagtgg tatcggtgta ccattcgcgc agacgaaaat tcttaacctt aatctggaat
     2221 acgccatgac cacacgcgtg attgctctcg acttagacgg caccttattg accccgaaaa
     2281 agaccctgct tccttcatcg atagaagccc tggcccgcgc tcgcgaagca ggctatcaat
     2341 taatcatcgt cacaggtcgc catcacgtcg ctattcatcc tttttatcag gcgctggcgc
     2401 tggatacacc tgctatttgc tgtaatggca cctatttgta tgattatcat gcaaaaaccg
     2461 tgctggaagc ggacccaatg cccgttaata aagctctgca actcattgag atgctgaatg
     2521 aacaccacat tcacggtctg atgtatgtcg atgatgcaat ggtctatgag cacccgaccg
     2581 ggcatgtcat tcgcacatct aactgggcgc aaaccctgcc gccggaacag cgtccgactt
     2641 tcacacaagt cgcttctctg gctgaaacgg cgcaacaagt taacgccgta tggaagttcg
     2701 ccctgacgca cgatgacctg ccgcaattgc agcattttgg taagcatgtc gaacatgaac
     2761 tgggactgga gtgtgaatgg tcctggcacg atcaggttga tattgcacgc ggcggcaaca
     2821 gcaaaggtaa acgtttgacg aaatgggttg aggcgcaagg ctggtcgatg gaaaacgtcg
     2881 tggcgttcgg cgataacttt aatgatatca gtatgctgga ggccgctggt acaggtgtgg
     2941 cgatgggcaa cgccgatgac gcggtaaaag cgcgcgccaa cattgtgatt ggtgataaca
     3001 ccaccgacag cattgcccag ttcatttata gccacctgat ttaatcaggc ggttatcgac
     3061 acacttttaa tttgcgcgta cagccacagg ccaggtttga tcgccagttc atccctagcc
     3121 cacgggctga tacgcgccca cagcgtttta ccgccgactt ccagctccac ttccacctgg
     3181 ccgttgtcgt cataactatt aacaactttt gcccgcaata cgttacgaat gctggtttgc
     3241 tgcggcggtt gtaaaaccag agaaacatcg gaagcctgaa tgcggatacg tagcgcagct
     3301 tgcaacggtt cgtccagctt attgacccac aaatgctgat cgcccagcgc cagcgcagtc
     3361 atcgcgtaat gcggatgatg ctcaagtacc gtcactttca gaatgctact ttgttgctct
     3421 ttcggcagcc acggattcat cacgctacta ccccagactt cttccagcgc gccaaaggct
     3481 ttcacctgac cgttttccag taccatcact ctgtctgcca gatggaggat ctcatccagc
     3541 gaatggctga catacaacat cgggatgttg atttcccgcg tcagacgttg cagataaggc
     3601 aacagttcgc gtttacgcgg aatatccagt gacgccagcg gttcatccag caacagcaat
     3661 tctggtgccg tcagcaaagc ccgcccaatc gctacgcgct gtttttcgcc tccggacagg
     3721 ctgcctggta aacggtcaag caacggttca atgcctaaaa gcgccaccag cttatcgaac
     3781 tgatcgacca tgtttttcgc catgccgtag cgcagattgc cacgcacttt gtaatgtgga
     3841 aacagccgcg catcctgaaa aacatagcca acgcgacgct tttctggcgt caggcagata
     3901 cctttttcgg catcatttag taaccgacca ttgaggacaa tcctcccttt ttgcgggcgc
     3961 gtcagtccac tgatggcgtt aatcagcgaa gtttttccgg caccggagac gccaaagata
     4021 gcagtgatgc cattggcggg cagcgtttca ttaatagtca ggcaatggtt gcccaacgtc
     4081 tgggaaaaat tcagttccag catgattagc gccccgcccg ttcacggctg attctggcca
     4141 gccattctga aatcaacagg gagatcatcg ccagcgcaat agaaataatg cacagtctcg
     4201 ctgcgccact ttccccgccg ggggtctgaa tcagggtata catggcagaa ggaatggttc
     4261 gcgtttcacc aggaatgttc gacacaaagg tgatggttgc gccaaattca ccgagagaac
     4321 gagcaaaagc cagtaccgta ccaacaataa ttcccggtaa ggtcagcggt aacgtgatag
     4381 taaagaaaac gcgccagcgc ccggccccca gtgttcttgc ggcctgttcc agtttgacat
     4441 caaccccttc cagcgccaga cgaattgccc gcaccatcag cggaaacgac atgacggcgg
     4501 cagcgagaac cgcgccgcgc cagctaaagg cgaaggtaat accaaaccag tcatacagac
     4561 gttcaccgat aaatccgcgc cgccccatcg aaactaataa taagtaaccg acgaccacgg
     4621 gcggtaacac cagcggtaga tgcagtacgc tgtcgagcag agctttgccc ggaaacgtgc
     4681 aacgcaccag taaccaggca aaaaagatcc caaacggcag gctaaacagc acagccaggg
     4741 aagaaacttt caggcttaat aaaactgcct gccattctgg atcggtcagt atcattactt
     4801 ggttgtaaat ccgtaacgtt taaagatttc cgctgcctgc ggtcccttca gataatcata
     4861 aaatgctttc actgtcgcat tgttatgccc ttccacaaca gcaaccggat attccacttt
     4921 tttatgtgaa tcttccggga aggtggcaac cacttttacc cctttgctgg caactgcgtc
     4981 agaaccgtaa acaatgccca acggcgcttc attgcgttcg accaacgcca gcgcgccacg
     5041 aacatcttcc gccggagcca gtttcgggga gagcgtatcc catgcgccca gtttttgcag
     5101 tgcttctttt gcataaatgc cagcgggaac atgttccgga tcgccaaccg ccaggcgacc
     5161 gccattcagc agtgaagtcc agttggtttt gctgtcgatg gtaaaatctt tctgctcgct
     5221 ggctttcggt gctacaacga ccaggctatt gccgagcagt gtctgacgcg tagctgtatc
     5281 gatcgctttt ttatcaaccg cataatccat ccatttctga tcggcagaaa taaacagatc
     5341 cgcaggcgca cccgcttcaa tctgacgggc gagagtagat gacgaagcga aagaagaaac
     5401 cacatccacg cctttctctt ttttatactg cgtagcaatg tcctgcattg cgttagtcag
     5461 tgatgcggcg gcgaacaccg tgattttccc ttcatctgct agtgcattgc cagcaacagc
     5521 gaaagagagt gccgccccgg caaacaagtt caaccattta cgagccattg gtaacccctt
     5581 aatgtaacgt tatgtaggcg acaatataac gactaactcg gaattttccc agcagttatt
     5641 gctaaccttt gtgaggtaga taagaaaaaa tatcggcagg aaaagcagga agttgagaaa
     5701 aagaaaatgc ccgactaagc gggcattcag ggaatcaatg attttgtccg ggctggtctt
     5761 ttttaccaac accagaaaag atgttgaata cttcaccaag accgtaaatc agacccagga
     5821 tgatggccat cacgacaggt accatgatta cggcgaatac cagacttttt aataactcta
     5881 acatggtcaa ctccagatat agtcatgaga ctattctaac cgctaagctc agaaaagcac
     5941 tccccttttg tgcggtcagc tttgcgtggc gttcgttttc cgtcacaata agacttttgc
     6001 taggacattg ttatgcaggc cgaaatcctt ctcaccctta agctccaaca aaaattattc
     6061 gccgacccgc gccgcatttc gctactaaaa cacattgcgc tttccggttc cattagccag
     6121 ggagcgaaag atgccggtat cagctataaa agcgcctggg atgccattaa cgagatgaat
     6181 cagttaagtg agcatattct ggtcgagcgc gcaacaggcg gtaaaggtgg cggcggcgca
     6241 gtactgaccc gctatggtca gcgactgatt cagctctatg acttactggc gcaaatccag
     6301 caaaaagcct ttgatgtgtt aagtgacgat gacgcccttc cgctgaacag cctgctggcc
     6361 gcgatctcac gtttttcact gcaaaccagc gcccgtaacc agtggttcgg taccatcacc
     6421 gcccgcgatc atgatgacgt tcaacagcat gttgatgtct tactggctga tggaaaaaca
     6481 cgcctgaaag tcgcaattac cgcacaaagc ggcgcgcgtc tggggctgga tgaaggcaaa
     6541 gaagtgttga tattgctaaa agcgccgtgg gtaggcatta ctcaggacga agcggtcgcg
     6601 caaaacgctg acaaccaatt accgggtatt attagtcata ttgaacgcgg cgcagagcag
     6661 tgcgaagtat taatggcgct accggacggg caaacactgt gcgccacagt gccggtaaat
     6721 gaagcgactt ctcttcagca aggacagaat gtcacggcct actttaatgc cgacagcgtg
     6781 attatcgcca cgctgtgcta agcgtgttga caatttgtta tgaaacacgt atccctgtca
     6841 gtaatcgctg cacaaaatgg gatataaaat gtcatcgttg caaattttgc aaggcacgtt
     6901 tcgtcttagc gacacaaaaa cgctgcaatt gcctcagcta acgttaaacg cgggtgatag
     6961 ttgggcgttt gtcggttcga atggaagcgg gaaatcggcc ctggcccgcg cgctggcggg
     7021 ggaacttccg cgtttgaaag gtgaacggca aagccagttt tcccacatca ctcgtctctc
     7081 cttcgagcaa ttgcaaaagc tcgtcagcga cgaatggcag cggaataaca ccgatatgct
     7141 cggccctggc gaagatgaca ccggacgcac tacggctgag attattcagg atgaagtaaa
     7201 ggatgcaccg cgctgcatgc aactggcaca gcagttcggt attaccgccc tcctcgaccg
     7261 acgctttaaa tacctttcca ctggcgagac gcgaaaaacc ctgctgtgtc aagcgctgat
     7321 gtcggagcct gacttgttga ttcttgatga gccgttcgat ggcctggatg ttgcctcacg
     7381 tcagcagctg gctgagcgac tcgcctcgtt acatcagtcc ggtattactc tggtactggt
     7441 gctcaatcgc ttcgatgaga tcccggaatt tgtccagttt gctggcgtgc tggcggattg
     7501 tacgttagcg gaaacgggcg ctaaagagga actgctccag caagcactcg tcgcgcaact
     7561 ggcgcatagc gaacagcttg aaggtgtgca actgccggag ccggatgaac cttcagcacg
     7621 tcacgcctta cccaccaacg aaccgcgcat tgtgctgaat aatggcgtgg tttcttataa
     7681 tgatcgcccc attcttaata accttagctg gcaggtgaat cctggcgagc actggcaaat
     7741 tgtcgggcca aatggtgcgg gaaaatcgac gttattaagc ctgattactg gcgatcatcc
     7801 gcaaggttac agcaacgatt tgacgctttt cggacgacgt cgtggcagcg gcgaaaccat
     7861 ctgggatatc aaaaagcata tcggttacgt cagcagtagt ttgcatctgg attaccgggt
     7921 cagtactact gtgcgtaatg tgattctttc tggctatttt gattcgattg gcatttatca
     7981 ggccgtttcg gatcgccagc aaaaactggt gcagcagtgg ctggatattc tcggcattga
     8041 taaacgcacg gctgacgctc cgttccatag tctttcctgg ggacagcagc gtctggcgct
     8101 gatcgtccgc gcactggtga aacatccgac gttgcttatt ctcgatgaac cactacaggg
     8161 gcttgatccg ctgaatcgcc agcttatccg ccgttttgtt gatgtgctga taagcgaagg
     8221 tgaaacgcaa ttgttgtttg tttcgcacca cgctgaagat gcgcctgcct gtattaccca
     8281 tcgtcttgag ttcgtgccgg acggtggact ctatcgctat gtgctgacaa aaatatattg
     8341 agtcggtagt gctgaccttg ccggaggcgg cctaagcgcc ctctccggcc aacggttcga
     8401 cgcatgcagg catgaaaccg cgtctttttt tcagataaaa agcgcaatca ttcataaacc
     8461 ctctgtttta taatcactta atcgcacata aaaaatggct aaactcttgt gtaaacgatt
     8521 ccactaattt attccatgtc acacttttcg catctttgtt atgctatggt tatttcatac
     8581 cataagccta atggagcgaa ttatgagagt tctggtcacc ggtggtagcg gttacattgg
     8641 aagtcatacc tgtgtgcaat tactgcaaaa cggtcatgat gtcatcattc ttgataacct
     8701 ctgtaacagt aagcgcagcg tactgcctgt tatagagcgt ttaggcggca aacatccaac
     8761 gtttgttgaa ggcgatatcc gtaacgaagc gttgatgacc gagatcctgc acgatcacgc
     8821 tatcgacacc gtgatccact tcgccgggct gaaagccgtt ggcgaatcgg tacaaaaacc
     8881 gctggaatat tacgacaaca atgtcaacgg tactctgcgc ttgattagcg ccatgcgcgc
     8941 cgctaacgtc aaaaacttta tttttagctc ctccgccacc gtttatggcg atcagcccaa
     9001 aattccatac gttgaaagct tcccgaccgg cacaccgcaa agcccttacg gcaaaagcaa
     9061 gctgatggtg gaacagatcc tcaccgatct gcaaaaagcc cagccggact ggagcattgc
     9121 cctgctgcgc tacttcaacc cggttggcgc gcatccgtcg ggcgatatgg gcgaagatcc
     9181 gcaaggcatt ccgaataacc tgatgccata catcgcccag gttgctgtag gccgtcgcga
     9241 ctcgctggcg atttttggta acgattatcc gaccgaagac ggtactggcg tacgcgatta
     9301 catccacgta atggatctgg cggacggtca cgtcgtggcg atggaaaaac tggcgaacaa
     9361 gccaggcgta cacatctaca acctcggtgc tggcgtaggc agcagcgtgc tggacgtggt
     9421 taatgccttc agcaaagcct gcggcaaacc ggttaactat cattttgcac cgcgtcgcga
     9481 gggcgacctt ccggcctact gggcggacgc cagcaaagcc gaccgtgaac tgaactggcg
     9541 cgtaacgcgc acactcgatg aaatggcgca ggacacctgg cactggcagt cacgccatcc
     9601 acagggatat cccgattaag gaacgaccat gacgcaattt aatcccgttg atcatccaca
     9661 tcgccgctac aacccgctca ccgggcaatg gattctggtt tcaccgcacc gcgctaagcg
     9721 cccctggcag ggggcgcagg aaacgccagc caaacaggtg ttacctgcgc acgatccaga
     9781 ttgcttcctc tgcgcaggta atgtgcgggt gacaggcgat aaaaaccccg attacaccgg
     9841 gacttacgtt ttcactaatg actttgcggc cttgatgtct gacacgccag atgcgccaga
     9901 aagcaacgat cctttgatgc gttgccagag cgcgcgcggt accagccggg tgatctgctt
     9961 ttcaccggat cacagtaaaa cgctgccaga gctcagtgtt gcggcattga cggaaatcgt
    10021 caaaacctgg caggagcaaa ccgtagaact ggggaaaacg tacccatggg tgcaggtctt
    10081 tgaaaacaaa ggcgcggcga tgggctgctc taacccgcat ccgcacggac aggtttgggc
    10141 aaatagcttc ctgcctaacg aagctgagcg cgaagaccgc ctgcaaaaag aatattttgc
    10201 cgagcagaaa tcaccaatgc tggtggatta tgttcagcgc gagctggcag acggtagccg
    10261 taccgttgtc gaaaccgaac actggttagc agttgtgcct tactgggctg cctggccgtt
    10321 cgaaacgcta ctgctgccca aagcccacgt tttacggatc accgatttga ccgacgccca
    10381 gcgcagcgat ctggcgctgg cgttgaaaaa gctgaccagt cgttatgaca acctcttcca
    10441 gtgctccttc ccctactcta tgggctggca cagcgcgcca tttaatggcg aagagaatca
    10501 acactggcag ctgcacgcgc acttttatcc gcctctgttg cgctccgcca ccgtacgtaa
    10561 atttatggtt ggttatgaaa tgctggcaga gacccagcga gacctgaccg cagaacaggc
    10621 agcagagcgt ttgcgcgcag tcagcgatat ccattttcgc gaatccggag tgtaagaaat
    10681 gagtctgaaa gaaaaaacac aatctctgtt tgccaacgca tttggctacc ccgccactca
    10741 caccattcag gcgcctggcc gcgtgaattt gattggtgaa cacaccgact ataatgacgg
    10801 tttcgttctg ccctgcgcga ttgattatca aaccgtgatc agttgtgcgc cacgcgatga
    10861 ccgtaaagtt cgcgtaatgg cagccgatta tgaaaatcag ctcgacgagt tttccctcga
    10921 tgcgcccatt gtcgcgcatg aaaactatca atgggcgaac tacgttcgtg gcgtggtgaa
    10981 acatctgcaa ctgcgtaaca acagcttcgg cggtgtggac atggtgatca gcggcaatgt
    11041 gccgcagggt gccgggttaa gttcttccgc ttcactggaa gtcgcggtcg gaaccgtatt
    11101 gcagcagctt tatcatctgc cgctggacgg cgcacaaatc gcgcttaacg gtcaggaagc
    11161 agaaaaccag tttgttggct gtaactgcgg gatcatggat cagctaattt ccgcactcgg
    11221 caagaaagat catgccttgc tgattgactg tcgctcactg gggaccaaag cagtttccat
    11281 gccgaaaggt gtggctgtcg tcatcatcaa cagtaacttc aaacgtaccc tggttggcag
    11341 cgaatacaac acccgtcgtg aacagtgcga aaccggtgcg cgtttcttcc agcagccagc
    11401 tctgcgcgat gtcaccattg aagagttcaa cgctgttgca catgagctgg acccaatcgt
    11461 ggcgaaacgc gtgcgtcata tcctgactga aaacgcccgc accgttgaag ctgccagcgc
    11521 gctggagcaa ggcgacctga aacgtatggg cgagttgatg gcggagtctc atgcctctat
    11581 gcgcgatgat ttcgaaatca ccgtgccgca aattgacact ctggtagaaa tcgtcaaagc
    11641 tgtgattggc gacaaaggtg gcgtacgcat gaccggcggc ggatttggcg gctgtatcgt
    11701 cgcgctgatc ccggaagagc tggtgcctgc cgtacagcaa gctgtcgctg aacaatatga
    11761 agcaaaaaca ggtattaaag agacttttta cgtttgtaaa ccatcacaag gagcaggaca
    11821 gtgctgaacg aaactcccgc actggcaccc gatggtcagc cgtaccgact gttaactttg
    11881 cgtaacaacg cagggatggt agtcacgctg atggactggg gtgcgacttt actttcagcc
    11941 cgtattccgc tttccgatgg cagcgtccgc gaggcgctgc tcggctgtgc cagcccggaa
    12001 tgctatcagg atcaggccgc gtttctgggg gcctctattg gtcgttatgc caaccgcatc
    12061 gccaatagcc gttatacctt tgactgtgaa accgtgacgc tttcgccaag tcagggcgtt
    12121 aaccagctgc acggcggccc ggaagggttc gacaaacgtc gctggcagat tgtgaaccag
    12181 aacgatcgtc aggtgctgtt tgccctgact tcagatgatg gcgatcaggg cttcccgggt
    12241 aatctcggcg cgacggtgca atatcgtctg accgacgata accgtatctc cattacttat
    12301 cgcgccacag ttgataaacc ttgcccggtg aatatgacta atcacgtcta tttcaatctt
    12361 gacggcgagc agtctgacgt gcgcaatcac aagttgcaga ttctggcgga cgaatatctg
    12421 ccggttgatg aaggcggcat tccgcacgac ggcctgaaat ctgtcgccgg aacgtctttt
    12481 gatttccgca gcgccaaaat catcgccagt gagtttcttg ccgacgacga tcagcgcaaa
    12541 gtgaaaggtt acgatcacgc gttcttgtta caggccaaag gcgatggcaa gaaagtggcg
    12601 gcgcatgtct ggtcagcaga tgaaaaattg cagctgaagg tctacaccac cgctccggct
    12661 ctgcaattct actccggcaa cttcctcggc ggcacaccgt cgcggggaac cgaaccttac
    12721 gccgactggc aagggctggc gttggaaagc gagtttctgc cggacagccc gaaccaccct
    12781 gaatggccgc aaccggactg cttcctgcat cctggtgaag agtattccag cctgacggaa
    12841 tatcagttta ttgctgagta agtttaagcc ctcgcgatga gggctttccc cttgtattgt
    12901 tcaaatcacc agcaaacacc gacatatttg caactcaata ttcacaacaa ccttacactg
    12961 cgacactatt ttcgctatgg ttatgcgtaa gcattgctgt tgcttcgtca cggcaatata
    13021 atgagaatta ttatcattaa aagatgattt gaggagtaag tatatggctg taactaagct
    13081 ggttctggtt cgtcatggcg aaagtcagtg gaacaaagaa aaccgtttca ccggttggta
    13141 cgacgtggat ctgtctgaga aaggcgtaag cgaagcaaaa gcagcaggta agctgctgaa
    13201 agaggaaggt tacagctttg actttgctta cacttctgtg ctgaaacgcg ctatccatac
    13261 cctgtggaat gtgctggacg aactggatca ggcatggctg cccgttgaga aatcctggaa
    13321 actgaacgaa cgtcactacg gtgcgttgca gggtctgaac aaagcggaaa ccgctgaaaa
    13381 gtatggcgac gaacaggtga aacaatggcg tcgtggtttt gccgtgactc cgccggaact
    13441 gactaaagat gatgagcggt atccgggtca cgatccccgt tacgcgaaac tgagcgagaa
    13501 agaactgccg ctgacggaaa gcctggcgct gaccattgac cgcgtgatcc cttactggaa
    13561 tgaaaccatt ctgccgcgta tgaagagcgg tgagcgcgta atcatcgctg cacacggtaa
    13621 ctcgttacgt gcgctggtga aatatctcga taacatgagc gaagaagaga ttcttgagct
    13681 gaatatcccg accggcgtgc cgctggttta tgagtttgac gagaacttca aaccgctgaa
    13741 acgctattat ctgggtaatg ctgacgagat cgcggcgaaa gcagcagccg ttgcgaacca
    13801 gggtaaagcg aagtaaacgt cattcgttta aaaatgagaa agccgactgc aagtgagtcg
    13861 gcttttttgt ttgtcgttgc taacaatgga gcacattgcc tgatgcgacg ctgcgcgtct
    13921 tgtcagggct acggtgattc atcggatacg ccactctgac ggcgcatccg acaattaaac
    13981 tttacccgcg acgcgctttt actgcattcg ccagttgacg taacagagca tcggtatctt
    14041 cccagccgat gcaggcatcg gtgatgctct taccgtaggc cagcggctcc ccgctctcca
    14101 ggctctgatt gccttcaacc agatgacttt ccaccatcac accgataatg gccttttcgc
    14161 caccggcaat ctgctggcaa acgtcagcac aaacatccat ctgctttttg aattgtttgg
    14221 acgaattagc atggctgaaa tcgatcatca cctgagctgg cagacctgct ttgttcagcc
    14281 cttctttcac ttcagcaacg tgcttcgcgc tgtagttagg ctctttaccg ccgcgcagaa
    14341 tgatatggca atcgccgtta ccgctggtat tcacaatcgc cgaatgcccc catttagtta
    14401 cggacaggaa gcagtgcggc gcaccggcgg cattaatggc atcgatagcc actttaatcg
    14461 taccgtctgt gccattttta aaacctaccg gacaagaaag accagacgcc agttcgcggt
    14521 gcacctgcga ttccgtggta cgtgcaccaa ttgcgcccca gctcatcagg tcagcgagat
    14581 actgaggagt gatcatatcg aggaattcac ccgcagctgg cagaccgctg tcgttaatat
    14641 cgagcagcaa tttacgggca atacgcaaac cgtcgttgat ctggaagctg ttatccatat
    14701 gcggatcgtt aatcagccct ttccagccca ccgtggtacg cggcttttca aaatagacgc
    14761 gcattacgat ttccagctca tctttcagct cttcacgcag cgccagcaag cgagtggcat
    14821 actctttagc cgcgacagga tcatgaattg agcacgggcc aatcaccacc aacaggcgat
    14881 catcattacc tttcaggatc ttatggatcg cttttcgggc atgggcaacc gtattcgcgg
    14941 cattttcagt agcggggaat ttttccagca atgcgacagg aggaagtaac tctttgattt
    15001 ctttgatgcg taaatcgtcg ttctgataat tcatgtctgt tccagtgttg ccatacttat
    15061 cttagtgaat gcaatacttc caatctatat cttccgtcag aatgtgtaaa cggggtttta
    15121 cactatgaac ggattaatcc tggaattagc aagaaaaacg ccagattgtc gcgaaaaacg
    15181 agatctctcc tacaatttct aactgtaact cctttgtttg ttaattattt caagattctc
    15241 tgctgcgttt cataacctgg ctgaaatctt aaaccaatgc cttatattca cctgcaaatg
    15301 cactgttgga agaggttatc cgacataacg accataacag gagcatccta tgaaaatgac
    15361 aaaactggcc acactatttc tgactgccac tctaagcctt gccagcggtg ccgcactggc
    15421 tgccgatagc ggagcgcaaa ccaataacgg ccaggcaaac gccgcagctg atgcgggcca
    15481 ggtagcccct gacgcccgtg aaaatgtcgc gccaaataac gtcgacaata acggggtaaa
    15541 taccggttct ggcggcacta tgctgcatcc ggatggttct tcaatgaaca atgacggcat
    15601 gaccaaagat gaagagcaca agaacaccat gtgcaaagat ggtcgctgcc cggatatcaa
    15661 taaaaaggta caaaccggtg atggcatcaa taatgatgtc gataccaaaa ccgacggtac
    15721 tacgcagtaa ccctgcaaaa tagccaataa tgggagagcg gagtctctcc cttttaattt
    15781 ttcccttcta aaagcagtat gatgtcataa agttaagtga gaataattaa gggatgactc
    15841 gtcatggcgc actcacactc acacacgtcc tcacacctgc cagaagataa taatgctcgt
    15901 cgcttgttgt atgctttcgg cgtgactgcc gggtttatgc tggtagaagt cattggtggt
    15961 tttctttctg gttctctggc tttgctggcc gatgcgggtc atatgttgac cgatactgcc
    16021 gccctgcttt tcgccctact cgccgtgcaa ttttcacgtc gtcctcccac cattcgccac
    16081 actttcggtt ggctaagact gaccacgctc gcggcgtttg tgaatgccat cgccctcgtg
    16141 gtaattacca ttttgattgt ctgggaggcg atagaacgct tccgcacgcc gcgtccggtc
    16201 gagggcggca tgatgatggc aattgccgtg gctgggttgc tggcaaatat actttctttc
    16261 tggttacttc atcacggcag cgaagagaaa aacctcaacg tacgagcagc ggcactgcat
    16321 gtgctcgggg acctgctggg ttcggttggg gctattattg ccgcgttgat tattatctgg
    16381 accggctgga cacctgctga ccccattctc tcgatactgg tgtcgcttct ggttctgcgt
    16441 agcgcgtggc gactgttgaa agatagcgtg aatgaattac ttgaaggtgc accggtatcg
    16501 ctggatatcg ctgaactgaa gcgtcgtatg tgccgggaaa tcccggaagt ccgcaatgtg
    16561 caccatgtgc atgtatggat ggtaggcgag aagccggtga tgacgttgca tgtgcaggtg
    16621 atcccgccac acgatcacga tgccttgttg gatcagatcc aacactacct gatggatcac
    16681 tatcagattg agcacgccac cattcagatg gaatatcaac cttgtcatgg gccagactgc
    16741 cacctgaatg aaggggtgtc tggccattca catcatcacc attaatggga cagcgcgcgt
    16801 gagcctcttt cgcgtgcgct attgatccac atccggctgc cgttgagcgc aataaaggtc
    16861 aggatgatgt actccagaga catggcgtaa acgccctgaa gtgcaaaaat aacgacgcta
    16921 atcacgttaa taatcaccca caacagccag ttttccacat acttacgcgt catcagaatc
    16981 attgccacga tagataacac catcatgcag gaatcccaga acgggaaagc gtccggttgc
    17041 agttcaggca tcgcaacctg taatcctaat gcttgcatga tgatgactgc cacgcgggtc
    17101 aaaaatgcaa acaccggatt gataaatacc gtcatcagac caatagcaat gacacaaacc
    17161 gccagccagg ttaatgcctt cggcaatggt aaccagcgaa ttttcaactc cgcctcgttc
    17221 tgactggttt gtcgcgacca cgcataccaa ccgtaaatat tcgcggcaaa gaaaaacacc
    17281 tgtaatagca ggctggcata cagctgaatc tgaaagaaaa taatgccaaa caaggtgacg
    17341 ttgatcaggc caaagaagta gttgctgatc ttctccagac tggcaaggcc aatacacagc
    17401 aacccggcga tcgtgcctac cgcttcgatc catgagagat cataaccgcc tgcccctatt
    17461 ggtatatgta ccaggatatt ctgcacacta aaaaaatcca tctttccccc aaagcgtaac
    17521 gttacgcctt attattcgtt atccacgtag tgtagccgca aaatccagca tacgattgag
    17581 cggcaccagc gccctctctc gcagcctttc atcaacatga acctcgtgat tgcttccttc
    17641 ctgttctaat gcctcagcga tagcctgaag gccattcatg gccatccacg gacaatgcgc
    17701 gcagctgcgg caggttgcgc cctcacccgc ggttggtgct tccagtaact ctttatctgg
    17761 caccgcctgc tgcattttgt agaaaatgcc ccgatcggtt gccacgataa gccgctgatg
    17821 tggcaatgtt tttgcagcag cgatcagttg actggtggaa ccgaccgcat ccgccatatc
    17881 aacaatagct tgcggtgatt ctggatgcac cagtatggca gcatccgggt attgtttttg
    17941 caagcgggtt aacgcctgag tcttaaactc atcatgcaca atgcaggcac cctgccagca
    18001 taaaatgtct gcgcctgtct gtttttgcac gtaacgaccc agatgtttgt cgggtgccca
    18061 gatgattttt tcacccaaac tatccagatg atcaataagt tcgacggcaa tgcttgaagt
    18121 taccacccaa tctgcgcgcg cttttaccgc agcagaagtg ttggcgtaga cgacgacagt
    18181 acgatcggga tgggcatcgc aaaatgcgtt aaattcttca acagggcagc cgagatccag
    18241 tgaacactca gcctgaagtg tcggcatcag aattgttttt tccggactga gaattttggc
    18301 ggtttctccc ataaatctca ccccagcgac taacaacgta gaagcgggat actttgcacc
    18361 gaagcgcgcc atttccaaag aatcagaaat acagccaccg gtttcttctg ccagttgttg
    18421 aatttcggga tcggtatagt agtgggcaac catcaccgca ttacgttctt ttagcagacg
    18481 ttttatcttc tcgcggtaat acgctttttc atcaatgctt aacggcgtcg gcttcggggg
    18541 gaaaggataa atcgccgtgt ctggatcaaa catcacgctc atcttaccat ctcgttttac
    18601 aggcttaacg ttaaaaccga cattagcgtg atattcgctg ttatcggtgt gatttgtttt
    18661 atatgctaaa caagatagtc gaatggtggg gaaaagtcac tcgaattacg tatgagatgc
    18721 cgaatgccac actttgcgtc ttattcctcc gacgtaaagt tgtttacgca gcaagcgcgg
    18781 agtatgtcat cagggtcaag aattgaaatt gagagatgcc tgaatagcaa aaaagcgcct
    18841 ttagggcgct tttttacatt ggtgggtcgt gcaggattcg aacctgcgac caattgatta
    18901 aaagtcaact gctctaccaa ctgagctaac gaccccttgc gggattatgc tgctttaatc
    18961 attcaggatg gtgggtcgtg caggattcga acctgcgacc aattgattaa aagtcaactg
    19021 ctctaccaac tgagctaacg acccatacgg gtgttacctg aaagatttct actcggaacc
    19081 aatctttaaa attggtgggt cgtgcaggat gactcgcttc gctcgcccta cgggccgttg
    19141 ctggcgcaac gttatccttc acgcttaaca tctgagtttg atgttaaaac tggtgggtcg
    19201 tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa
    19261 cgacccgagt ggtgggtgat gacgggttcg aaccgccgac cccctccttg taagggaggt
    19321 gctctcccag ctgagctaat cacccgatac tgcgctggat actacaaact acttccaccg
    19381 aacccaccgt aattagtggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    19441 gtcaactgct ctaccaactg agctaacgac ccggtggtgg gtgatgacgg gatcgaaccg
    19501 ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc gatactacgc
    19561 tggatactac aactaactag gttagtggtg ggtcgtgcag gattcgaacc tgcgaccaat
    19621 tgattaaaag tcaactgctc taccaactga gctaacgacc cactttttcg ttgctttcgg
    19681 tttgtttgat atcccgtggc aacggcggca tatattactg atttcagact tgagcgcaac
    19741 aaaaatttcg atgtagatca ctcaactgct tatgattcgc acgacacgac cagaaataat
    19801 gcgacttctg gtcgtgtgtt atgcattaca tcgcgttcag acgtttttgc gcctgtttag
    19861 cgccatcagt accagggtat ttactgataa cctgctggta tacggctttc gcttttgcgg
    19921 tgtcaccttt gtcctgcatg atgacgccga ctttaaacat cgcatctgca gcctttggtg
    19981 acttcggata gtttttcact accgaagcaa aatagtacgc cgcatcatct tttttaccct
    20041 tgttgtagtt taactgaccc agccaataat tggcgtttgg caggtaagtt gaatcagggt
    20101 aatttttgat gaaattctga aatgccacca ttgcgtcatc ctggcgggat ttatcctgca
    20161 ccagcgcaat agctgcattg taatccgtgt ttgcatcacc gcttttcacc ggcgcgccag
    20221 cattcgcagt accagcatca gctgtcggcg tcgttgatgc cgccgcaccg ctttgatcac
    20281 cgctggttga ttgcgccgct gcaccaccgc tgctgaggct gtcgatctgc aacaggatct
    20341 gcttttgccg ctccacgacc tgattcagtt gatactgatt ttcctgaatc tgaccacgca
    20401 gggaatcaat atcggattga ttatcagaga gttgttgctg gagttgggtt aaaagctggc
    20461 tgtgagcatt agaaatacgc tcaagttgag tgacgcggtc ttcgaccgag cctgagccga
    20521 cactactgat tggtgcctga gcaaaagcgg cccagggggc cgctatacca accagtaacg
    20581 acagactcaa tagttgatgt ctgaagttac tgctcatgca attctcttag taaaccagta
    20641 ccgcacgacg gtttttggag tatgccgctt cgtcatgacc cagtactgca ggtttttctt
    20701 taccgtaaga aacgatggag atctggtctg cagaaacgcc tttaccctgc aggtacatct
    20761 taacggcgtt cgcacgacgt tcacccaggg agatgttgta ttccggagta ccacgttcgt
    20821 ccgcgtgacc ttctacggtg actttgtaag acgggttgct acgcaggaag tttgcatgtg
    20881 catccagcat ttgagcgaag tcagaacgga tatcgtactt gtccagatcg aagtaaacga
    20941 tgttgttctg ctgcagctgt tgcatttgca gacgagcctg ctcttcggaa gacatgttgc
    21001 cgttgccgcc gttcgcatcc ataccagtgc cggcacccag catgccttcg ctgccgtcat
    21061 tgctggcgtt cttgttggaa gaacatgccg caattgccat aacaggcaga gcaatcatca
    21121 gccctttcag cactttgttc agttgcattt caatgattcc tttactattc aattaattat
    21181 tatcacagat acggcgacca ggcagggaat ttgacctgtc catcagttgc cggaagacgc
    21241 gctttgaaac gcccatctgt agaaaccaaa ttcagcacgg atcccatccc ctgagaagag
    21301 ctgtagatta ccatagtgcc gttaggtgcc agacttggcg tttcatccag gaacgtggac
    21361 gacagaactt gtacgcctcc cgttgccaga tcttgtttgg caatgtgctg ctgcccaccg
    21421 ttggagctga ccattaccat aaatttaccg tcgctgctga catccgcatc ctggttctgc
    21481 gaaccttccc aggtaatacg ttgtggcgca ccgccgttga tattcacttt ataaacctgt
    21541 ggacgaccgg cctggtcaga agtaaatgcc aggttctggc tgtccgggaa ccaggtcggt
    21601 tcggtattgt tactgcgacc atcagtcacc tggcggatct ggccagaagc caaatccatt
    21661 acgtacaggt tcaggctacc ggttttcgac aaggcgaatg ccagtttgct gccgtctggc
    21721 gagaatgcag gcgcaccgtt gtgacgcggg aatgaagcca cctggcgtac agcgccgttt
    21781 gccagcgttt ggataaccag cgcggaacga ccgctttcga aggtcacata agccagttta
    21841 gaaccgtctg gtgaccacgc cggggacatc agcggctgtg gtgaacggtg aacgacaaac
    21901 tggttgtaac cgtcatagtc agatacgcgc agttcatacg ggaactgacc gccgttggtc
    21961 tgaacaacgt aggcaatacg ggtacggaac gcacctttaa taccggtcag tttttcaaac
    22021 acttcatcac tggcggtatg accagcataa cgcagccact gcttgttcac tttgtacgag
    22081 ttctgagcaa gtacagtacc cggtgcgccg ccagtgtcaa caagttgata agcaacattg
    22141 taagaaccat ccggattcgg agtgacctga ccgacaacta cagcgtcaat acccagcgcg
    22201 gaccatgcag ctggttgtac ttcctgcgca ctacccggct gctgtggcag acgagcgcga
    22261 tctaacggat taaatttacc gctgttacgc aagtcagcag caacgatgcc gccaatatct
    22321 tcaggtgccg caccagggcc cgcccactgg aaaggaacaa ctccaatagg acgaccggaa
    22381 tctacaccgc tgtcgatcac aatgcggact tcagcatgca gaactgatgc ccacagtatg
    22441 agaaaaccaa atgctactcg taatgcctgc ttcatcatat ctcccttatc tggaccgatg
    22501 gcccacgata atttagcaga atgttaacaa actaaaatac acaaaactac cagaaccccg
    22561 tggcaaccgg tgcctgatgt tgaccgtcgg aacagtcaac atcgcgatta cggtttgaag
    22621 tccaatggcg cgtttttgaa cacttcatat actgcctggc ttggtggttt cgggatcttc
    22681 gcaagtttag ctgctgccaa cgcagcctga caaagtgcgg gatcgccacc ttcaggtttg
    22741 atatccagta acataccatc gggtgccagt tttatgcgca gcgtacaggt tttgcctgca
    22801 taggacgatg cgtcatagaa cttactttcg atagcagatt taatctgccc ggcatagtta
    22861 ttgatatcgg cccctgatgc gccattgttt ttagtattac cactcccggc aggcgaagca
    22921 ttgttccctt tcgcccctcc ccccgttttc ggtgcattct taccagagct tagctcaccg
    22981 aaaatatcat ctgcctctgc ggcagctttt gctgcagcgg ctttttcagc tgctgctttt
    23041 gcagctgccg cttttttgtc tgcggcggct ttttctgccg ctgctttctt atcagctgct
    23101 gccttttctg cagccgcttt ttctgctgct tttttgtcgg ctgcagcttt ctctgctgcc
    23161 gctttcttat cagctgcagc cttttcagca gccgctttct tctcagcttc tgccttggct
    23221 ttttcagcag cttcagttgc cgctttcttt cttgcttcag ctgcagctgc ttctgccgct
    23281 tccgctttct tcttcagcgc cgcagcggct gcctcggctt ttttctgcgc ttcggctgcg
    23341 gctttggcgg cttctgcttc tgcttttttc ttcgcatctg cagccgcttt cttcgctgca
    23401 tcttccgcag ctttagcatc cgcttcggcc ttcgctttag catctgccgc cgctttcgct
    23461 gccgcctctt ccgcttgctt ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc
    23521 tttttctgtt cctgagcagc taaccgctct ttctcaagtt gcttcaggcg ttcctgttca
    23581 gccgcttgtt tctcacgcag ttcttcagca gcctgctgtt ccttcatctt gcgctgctca
    23641 tcagaacgct tcgcgcttga ttcctggctt tgcatgcgtt tgtactgctc aactaccgca
    23701 cctgaatcaa ccatgacagc gtcgatggac gaaccaccgc cgcctccagc tgaagcttct
    23761 atattctcat cgaacgaact ccagatcagc gccgcaaata agatgacatg cagcactgct
    23821 gaaataatta tcgcccgctt gagcttgtcg ttttgttcgg ttgcctttga cactctcggt
    23881 ttccaaaaac tgttcgcctg ttacccgctc tctttcaagc aagggaaacg cagatgttta
    23941 gataggctgc gtcattaaac caaccgattt cacacccgca ctatgtaaca agttcagtgc
    24001 tttaattatt tcatcgtaag gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg
    24061 gttggccttg aaacggctgg acacttccgc caccacctgc tctggtggta aacgctccag
    24121 gcgatctttc tcaaccacca cggtgtactg accaatacca gacacttcaa caatcactgg
    24181 cggattatcg ttactgctca ccgcctgtga ttcagtagcg tctggcagat cgacctccac
    24241 gctctgggtg atgatgggcg ctgtcgccat aaagatcagc aacagcacca gcagtacgtc
    24301 cagcaacggt acaatgttga tttcggactt gagatcgcga cgacctcgtc cacgcgctct
    24361 ggccatggct taccccttgt tgctctcgct aacggtaaac gcctggcggt gcagaatcgc
    24421 ggtaaactct tccataaagt tgtcgtaatt cagttccagt ttgtttacgc gttggttgag
    24481 acggttataa gccattaccg ccggaatagc ggcgaacaga ccgattgctg tagcaatcaa
    24541 cgcttctgcg atgcctggcg caaccatttg cagcgttgct tgttttaccg ccccgagggc
    24601 gataaaggcg tgcatgatcc cccagaccgt accaaacaga ccaatatacg ggctgatgga
    24661 gccaaccgta ccgaggaacg gaatgtgcgt ttccagattt tcaagttcac ggttcatgga
    24721 aatacgcatc gcacgcgacg ccccttctac tacggcttcc ggcgcatggc tattggcacg
    24781 atgcaggcgc acaaattctt tgaacccgct gtaaaagatt tgttccgaac cggtcagatt
    24841 atcccgtttc ccctggctct cttgatagag gcgagagagt tcgattccag accagaattt
    24901 atcctcaaac gcttcggctt cgcgcgccgc tgcgttaaga atacgggtcc gctggataat
    24961 aatggcccag gatgcgattg aaaaaccaat caaaatcaac atgataagtt taaccagaag
    25021 gctagccttc aggaacaaat caaggatatt catgtcagtc actgcttaaa ctccgcgaca
    25081 atagacttgg gaagcgcacg aggcttcatt ttgagtgggt caacgcaaac aaccagaacc
    25141 tctgcttcat tcagcaaagt attctcggcg ttgacaatac gttgcgtgaa aaccaaagag
    25201 gtgccacgca ttgatgttat ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt
    25261 gcgtaatatt ccaccgtcat tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc
    25321 tgctgactga agtgatgatg acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg
    25381 taactggcgt ggtacaccac accaccggca tcggtatctt catagtagac gcgaaccggc
    25441 catcgaaaca gcgttgtatt cactttacat cccggtaatg caacaaaagt tagagctttt
    25501 aaacgctgct actatacgcg cgcgattgag gtttgggaat gagggcaagt taagggagcg
    25561 taaaaattta tgggcctgga gcggcccata attttttaaa gagaattctg atttaaaaga
    25621 agaagaaaat cagcccgaca atgagaacaa tatcggcaag caatgggcaa aaaatccctt
    25681 gccaaagaac cttctgcgga cgaaagccca cgccgtgaat cacaccagca cagacggccc
    25741 acatcagcaa taaaccatgc cagatttcca gttcactggt cttcgcggcg aaacgagatg
    25801 ggtcccaaaa catacatcct gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg
    25861 ggcgcttgtc cattaccgca tacaaggttg cgataatctt actcatcaga tgtcttcttg
    25921 accggctttg cctgattcga cgtgctcaag cgccagcgcg gtgattaccc caaacgaaca
    25981 ggcaagaagc gttcccagaa tccatgcgaa ataccacatt tttagctcct tacttagtac
    26041 agagagtggg tgttacgttc aatatcttct ttggtgatac gaccgaacat tttccagtaa
    26101 caccaggcgg tgtagagcag aatgatcggt accagaacca ccgcaaccca ggtcatgacg
    26161 ttaagtgtca gctggctgga agttgcatcc cacattgtca gacttgcgtt catcatggtg
    26221 ctggacggca tcacaaacgg gaacattgcg ataccggcag tcaggatgat gcaggccagc
    26281 gtcagggagg agaacacaaa cgcccacgcg gctttatcca tacgtgcagt caggatggtc
    26341 agcagcggca gaaccacacc cagtgccgga atagcccaca gaattggcgt gttgttgaag
    26401 ttaaccagcc atgcgccagc ttcacgaacc acttctttat tcagcgggtt agaggctgcg
    26461 taatggtcca ttgtcgattt cacgacataa ccatcgatac cgtacatcac ccatacgcca
    26521 gctagtgcga agcagaccag tgtcaccagt gccgccacct gagccgttgc acgggtacgc
    26581 aggtgcagtt cgcccacggt acgcatctgc agataagtcg cgccctgagt aatgatcatc
    26641 cctacgctca ccacgcctgc cagcaggccg aacgggttaa gcaactggaa gaagttaccg
    26701 gtgtagtaca gacgcagata ttcatcaacg ttgaacggta cgccctgcaa caggttaccg
    26761 aacgctacac caatcaccag cggcggaacg aagctaccaa tgaagatgcc ccagtcccac
    26821 atgttacgcc agcgggtttc ttcaatcttg gagcggtagt caaaaccgac cggacggaag
    26881 aacaaagacg ccagcacgag gatcatcgcc acatagaagc cggaaaacgc agcggcatag
    26941 accatcggcc aggcagcaaa cagtgcgccg cctgcggtga tcagccaaac ctggttaccg
    27001 tcccagtgtg gtgcgatgga gttaatcata attcgacgct cggtgtcgtt acgaccgagg
    27061 aaacgggtga gcatgcccac ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc
    27121 agaacgccaa ccagcagcca ccagataaaa cgcaatactt cataatcgat catttgacga
    27181 ctcctgtctt agcgtgccgg ctgagtagtc gtggaagact gctcaaagtg atagcgaccg
    27241 gttttcaggc tgcttgggcc gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc
    27301 aggaacaggg tatacaggcc gcaaatcagc accattgaga agatgagatc gcctgcggtc
    27361 agtgacgagt tcgccacagc tgtcggcagc acttcaccga tagcccacgg ttggcggcca
    27421 tattcagcca cgaaccagcc cgcttctaca gcaatccacg gcagcggaat accgtacagc
    27481 gcggcgcgca gcagccattt tttctcgcca atgcggttgc ggatgacact ccagaaagag
    27541 agcgcgatga ttgccagcag caggaagcca cacgccacca tgatacggaa cgcgaagtac
    27601 agcggcgcta cacgcggaat ggagtctttg gttgcctgtt gaatctgagc ttcagtcgca
    27661 tcagccacgt ttggcgtata gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg
    27721 ctattgaact ggtcacgaac cgcctggtcg gtagaaccag aacgcagttg ttcgagcaga
    27781 gagtacgcct tcatcccgtt acgaatgcgt tcttcatgct gcaccatcag ttctttcagg
    27841 ccgataactg gagtatccac ggaacgcgtt gcaatgatgc ccagcgcgta agggatctgg
    27901 atcgcaaatt tgttcgtctc ctcttcctga tcaggaatgc cgaacagagt aaaggcagca
    27961 ggcgcaggtt gcgtttccca ctcagcttca atagcagcca gtttggtttt ctgcacgtcg
    28021 cccatttcgt aaccagattc atcacccaga acaataacag acagaacggc agccataccg
    28081 aagctggcag cgatagcaaa ggagcgttta gcgaaggcga agtcacgacc tttcagcata
    28141 taccatgcgc tgataccgag gatgaacatc gcgccagtaa cataaccaga cgctacagtg
    28201 tgaacgaatt tcacctgagc aaccgggtta agcaccagct cggagaagct caccatctcc
    28261 atacgcatag tttcaaagtt gaaatcggac gcgattgggt tttgcatcca gccgttcgca
    28321 accagaatcc acagtgcgga caggtttgaa ccgagcgcca ccagccaggt gacacacata
    28381 tgctgaactt tacccagacg atcccaaccg aagaagaaca gacctacaaa ggtggattcg
    28441 aggaagaagg ccatcagacc ttcgattgcc agcggcgcac cgaagatatc ccctacataa
    28501 tgggaatagt aagaccagtt agtcccgaac tggaactcca tggtcagacc ggtagccaca
    28561 cccagagcga agttgatacc aaacaacttg ccccagaact tggtcatatc tttataaatc
    28621 tgtttgccgg aaaggacgta gaccgtttcc ataatggcca gcaggaacgc cataccgagc
    28681 gtcagtggca caaaaaggaa gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt
    28741 tcgactatat ctaacatcat gactccttgc tcatcgcatg aagactccga gagtgaaccc
    28801 cgttagaaag ggtcacacgc atgccccaat acaaaattat ttgctcgcct ctatcgttgc
    28861 catttcatca aaagaggaaa agacaatgat
//
LOCUS       DAZUCE010000037        28873 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZUCE010000037 DAZUCE010000000
VERSION     DAZUCE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.037"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            80..739
                     /gene="qseB"
                     /locus_tag="ACHY03_001427"
     CDS             80..739
                     /gene="qseB"
                     /locus_tag="ACHY03_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFS3904674.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGCQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            736..2085
                     /gene="qseC"
                     /locus_tag="ACHY03_001428"
     CDS             736..2085
                     /gene="qseC"
                     /locus_tag="ACHY03_001428"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFS3904675.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            2195..2776
                     /gene="mdaB"
                     /locus_tag="ACHY03_001429"
     CDS             2195..2776
                     /gene="mdaB"
                     /locus_tag="ACHY03_001429"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFS3904676.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            2807..3121
                     /gene="ygiN"
                     /locus_tag="ACHY03_001430"
     CDS             2807..3121
                     /gene="ygiN"
                     /locus_tag="ACHY03_001430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFS3904677.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(3180..5072)
                     /gene="parE"
                     /locus_tag="ACHY03_001431"
     CDS             complement(3180..5072)
                     /gene="parE"
                     /locus_tag="ACHY03_001431"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFS3904678.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(5101..5682)
                     /gene="yqiA"
                     /locus_tag="ACHY03_001432"
     CDS             complement(5101..5682)
                     /gene="yqiA"
                     /locus_tag="ACHY03_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFS3904679.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(5682..6509)
                     /gene="cpdA"
                     /locus_tag="ACHY03_001433"
     CDS             complement(5682..6509)
                     /gene="cpdA"
                     /locus_tag="ACHY03_001433"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFS3904680.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(6534..6956)
                     /gene="yqiB"
                     /locus_tag="ACHY03_001434"
     CDS             complement(6534..6956)
                     /gene="yqiB"
                     /locus_tag="ACHY03_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFS3904681.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6957..7586)
                     /gene="nudF"
                     /locus_tag="ACHY03_001435"
     CDS             complement(6957..7586)
                     /gene="nudF"
                     /locus_tag="ACHY03_001435"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFS3904682.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7791..9272
                     /gene="tolC"
                     /locus_tag="ACHY03_001436"
     CDS             7791..9272
                     /gene="tolC"
                     /locus_tag="ACHY03_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFS3904683.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            9420..10091
                     /gene="ygiB"
                     /locus_tag="ACHY03_001437"
     CDS             9420..10091
                     /gene="ygiB"
                     /locus_tag="ACHY03_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFS3904684.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            10097..11257
                     /gene="ygiC"
                     /locus_tag="ACHY03_001438"
     CDS             10097..11257
                     /gene="ygiC"
                     /locus_tag="ACHY03_001438"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFS3904685.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(11295..12083)
                     /gene="ygiD"
                     /locus_tag="ACHY03_001439"
     CDS             complement(11295..12083)
                     /gene="ygiD"
                     /locus_tag="ACHY03_001439"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFS3904686.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            12226..12999
                     /gene="zupT"
                     /locus_tag="ACHY03_001440"
     CDS             12226..12999
                     /gene="zupT"
                     /locus_tag="ACHY03_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFS3904687.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(13057..13227)
                     /gene="yqiD"
                     /locus_tag="ACHY03_001441"
     CDS             complement(13057..13227)
                     /gene="yqiD"
                     /locus_tag="ACHY03_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFS3904688.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(13489..14142)
                     /gene="ribB"
                     /locus_tag="ACHY03_001442"
     CDS             complement(13489..14142)
                     /gene="ribB"
                     /locus_tag="ACHY03_001442"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFS3904689.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(14247..14395)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            14517..14816
                     /gene="ubiK"
                     /locus_tag="ACHY03_001443"
     CDS             14517..14816
                     /gene="ubiK"
                     /locus_tag="ACHY03_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFS3904690.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     gene            complement(14851..15051)
                     /gene="glgS"
                     /locus_tag="ACHY03_001444"
     CDS             complement(14851..15051)
                     /gene="glgS"
                     /locus_tag="ACHY03_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFS3904691.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            15320..15949
                     /gene="yqiJ"
                     /locus_tag="ACHY03_001445"
     CDS             15320..15949
                     /gene="yqiJ"
                     /locus_tag="ACHY03_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFS3904692.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            15976..17637
                     /gene="yqiK"
                     /locus_tag="ACHY03_001446"
     CDS             15976..17637
                     /gene="yqiK"
                     /locus_tag="ACHY03_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFS3904693.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            17876..17935
                     /locus_tag="ACHY03_001447"
     CDS             17876..17935
                     /locus_tag="ACHY03_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFS3904694.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            18252..18311
                     /gene="ibsE"
                     /locus_tag="ACHY03_001448"
     CDS             18252..18311
                     /gene="ibsE"
                     /locus_tag="ACHY03_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFS3904695.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(18431..19864)
                     /gene="hldE"
                     /locus_tag="ACHY03_001449"
     CDS             complement(18431..19864)
                     /gene="hldE"
                     /locus_tag="ACHY03_001449"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFS3904696.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(19912..22752)
                     /gene="glnE"
                     /locus_tag="ACHY03_001450"
     CDS             complement(19912..22752)
                     /gene="glnE"
                     /locus_tag="ACHY03_001450"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFS3904697.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESHPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGERAIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(22775..24076)
                     /gene="ygiF"
                     /locus_tag="ACHY03_001451"
     CDS             complement(22775..24076)
                     /gene="ygiF"
                     /locus_tag="ACHY03_001451"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFS3904698.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQIGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            24318..24938
                     /gene="ygiM"
                     /locus_tag="ACHY03_001452"
     CDS             24318..24938
                     /gene="ygiM"
                     /locus_tag="ACHY03_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFS3904699.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            25002..26240
                     /gene="cca"
                     /locus_tag="ACHY03_001453"
     CDS             25002..26240
                     /gene="cca"
                     /locus_tag="ACHY03_001453"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFS3904700.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(26421..27242)
                     /gene="bacA"
                     /locus_tag="ACHY03_001454"
     CDS             complement(26421..27242)
                     /gene="bacA"
                     /locus_tag="ACHY03_001454"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS3904701.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(27333..27701)
                     /gene="folB"
                     /locus_tag="ACHY03_001455"
     CDS             complement(27333..27701)
                     /gene="folB"
                     /locus_tag="ACHY03_001455"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFS3904702.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            27806..28423
                     /gene="plsY"
                     /locus_tag="ACHY03_001456"
     CDS             27806..28423
                     /gene="plsY"
                     /locus_tag="ACHY03_001456"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFS3904703.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(28436..>28873)
                     /gene="ttdR"
                     /locus_tag="ACHY03_001457"
                     /gene_synonym="dan"
     CDS             complement(28436..>28873)
                     /gene="ttdR"
                     /locus_tag="ACHY03_001457"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFS3904704.1"
                     /translation="KRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELG
                     NGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQ
                     SANIWAVYREPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
BASE COUNT         7147 a         7805 c         7187 g         6734 t
ORIGIN      
        1 aaataaaatt tagtgctgta cagagcgcgt tacaacacgg tttactggca gcaaatacgg
       61 ttatcgcagg gatgaaaaaa tgcgaatttt actgatagaa gatgacatgc tgattggcga
      121 cggcatcaaa acgggcctta gtaaaatggg ttttagcgtc gactggttta cacaaggttg
      181 tcagggaaaa gaggcgcttt atagcgcacc ttatgatgcg gtgatcctgg atttaacctt
      241 accaggcatg gatggtcgcg atattttgcg cgaatggcga gaaaaaggtc agcgtgagcc
      301 ggtactgatc ctgaccgcgc gcgatgcgct ggaggaacgt gtagaagggc tgcgtctggg
      361 agctgacgat tatctgtgta aaccttttgc gttgatagaa gtcgccgcca ggctggaagc
      421 tctgatgcgc cgaaccaacg gccaggccag caacgagctg cgccacggta acgtcatgct
      481 cgaccccggc aaacgtatcg ccacgctggc tggcgaaccc ttaacactga aaccaaaaga
      541 atttgccctg ctggaattac tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat
      601 tgaagagaaa ctgtatacct gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt
      661 gcatcatctg cgacgcaaac tcggtagtga ttttattcgt accgtgcatg gtattggcta
      721 cacattaggt gagaaatgaa atttacccaa cgtcttagtc tgcgtgtcag gctgacgcta
      781 atctttttaa ttctggcctc ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa
      841 acaacggata acgtcgatga attgttcgac acccaactga tgctgtttgc caagcggtta
      901 agtacgctcg atctcaacga aatcaacgcg gcggatcgca tggcacagac gccaaataaa
      961 ttaaaacacg gtcatgttga tgacgatgcg ctgacctttg ccatctttac ccacgacggc
     1021 agaatggtcc ttaacgatgg cgataacgga gaagatattc cctatagcta tcaacgggaa
     1081 ggttttgctg acgggcaact ggtgggcgat aaagatcaat ggcgttttgt ctggatgacc
     1141 tcacctgatg gcaaatatcg cattgttgtt ggtcaggaat gggaataccg tgaagacatg
     1201 gcgctggcga ttgttgccgg gcaattgatc ccgtggttgg tcgcactgcc gattatgtta
     1261 atcatcatga tggtactact gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca
     1321 ctacgtatgc gtgaccctga ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa
     1381 gtgcgtccac tggttgagtc gttgaatcaa ctgttcgccc gcacacatgc gatgatggtt
     1441 cgtgaacgac gctttacctc tgacgcagct cacgaacttc gtagcccgtt aacggcgctg
     1501 aaagtgcaaa ccgaagttgc gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca
     1561 ctgctccaat tacattccgg gatcgatcgc gctactcgtc tggttgatca actgctcacg
     1621 ctatcgcggc tggactcact ggataacctt caggacgtcg cggagatccc gcttgaagat
     1681 ctcctgcaat cgtcggtgat ggatatttac cacacggcgc agcaggcgaa aattgacgtg
     1741 cgactgacac tcaatgtcca gggcatcaaa cgcaccggac aaccgctatt gctaagtttg
     1801 ttggtgcgaa atttgctgga taacgccgtg cgttacagtc cacagggcag cgtggtggac
     1861 gtcacgctga atgccgataa tttcatcgtg agggataacg gccccggtgt gacaccagag
     1921 gcactggcgc gaattggcga acgcttctat cgcccacccg gacaaaccgc taccggcagt
     1981 ggacttgggc tatcgattgt ccagcgaatc gccaagctgc atggcatgaa tgttgagttt
     2041 gggaatgcgg aacaaggtgg atttgaggcg aaagtaagct ggtaagggta agacttttgc
     2101 taaattcgca aaagactttg cacattttgc taatttcacc gtaccgctct gtgacgtact
     2161 atagtcggca aacgtctcac cttgaggtta aaaaatgagc aacatcctga ttatcaacgg
     2221 cgcgaaaaaa ttcgcccact ccaatggtca actgaacgac accctgaccg aagtcgcgga
     2281 tggcacactg cgcgacctcg ggcatgatgt tcgcatcgtt cgcgccgaca gcgactacga
     2341 tgtcaaagcg gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg
     2401 ctggtggatg ggcgcaccgt ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg
     2461 tcacgggact ctgtatgcca gcgatggtcg tacccgcaaa gatccgtcga aaaaatacgg
     2521 ttccggcggc ctggtacagg gcaaaaaata tatgctttct ctgacctgga acgcgccaat
     2581 ggaagccttc accgaaaaag atcagttctt ccacggcgtt ggcgttgacg gtgtgtatct
     2641 gccgttccat aaagcaaacc aattcctcgg tatggaaccg ctgccgacat ttatcgctaa
     2701 tgacgtgata aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt
     2761 ggaaattttt ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat
     2821 cgcagaaatc cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc
     2881 taaaatcgtt ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga
     2941 ttgcgctgct ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgattgagca
     3001 gtgggaaagc atcgcgcatc ttgaagcgca tctgcaaacc ccgcacatga aggcgtatag
     3061 cgaagccgta aaaggtgacg tgctggagat gaatattcgt attctgcagc cagggattta
     3121 aacctgcctt gtttgcccgg ccatcctgac cgggcaatgt tctttccttt aaacctcaat
     3181 taaacctcaa tctccgccat gtcgcctttc tcttgcaacc agttgcggcg atcttccgag
     3241 cgtttcttcg ccagcagcat atccatcatc gcgtcagtac gctgatcgtc ttcatcatcg
     3301 atagtcaact gcaccagacg gcgagtgttc ggatcaagcg tggtttcacg caattgcatc
     3361 gggttcattt cccccagacc tttaaaacgc tggacgttcg gcttgccttt cttgcgtttt
     3421 aattgctcaa gtacgccctc tttctcttct tccgtcagcg cgtaataaac ctctttcccg
     3481 agatcaatac ggtagagcgg tggcagtgcg acgtaaacgt gaccgtgttt caccaacgcg
     3541 cggaagtgtt ttacaaacaa agcgcagagc agcgtggcaa tgtgcagacc atcggagtca
     3601 gcatccgcca ggatacagat cttgccgtaa cgaagctggc tcaaatcgtc gctgtcagga
     3661 tcgataccga tcgctaccga aatatcgtgc acttcctgcg aagccagcac ttcgtcggaa
     3721 gagacttccc aggtgttaag gatcttacct ttcagtggca tgatcgcctg atattcgcga
     3781 tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt ccacgaggaa cagttcggta
     3841 cggttaaggt cctgcgcggt acaatcagcc agtttgccag gcagcgccgg gccgctggtc
     3901 agctttttac gcaccacttt tttagccgca cgcatacgac gctgagcgct ggaaatcgcc
     3961 atctctgcca gcaactccgc cgcctgaacg ttctggttca gccacaggat aaaggcatct
     4021 ttcaccacgc cagaaacgaa tgccgcgcac tgacgcgaag agagacgctc tttggtctgc
     4081 ccggcaaact gcggatcctg cattttcacc gacagcacat aggcgcagcg atcccagata
     4141 tcttccgccg acaactttac accgcgcggc agaatgttgc ggtattcaca gaactcacgc
     4201 atcgcgtcca acaggccctg acgcagaccg ttaacatggg taccgccctg catcgttggg
     4261 ataaggttga cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca cagtagcgcc
     4321 cagtccacag cttcagtatc accagcgaaa ttaccgataa acggtttttc tggcagcgtc
     4381 ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac cgtcctgata gcaccagcgc
     4441 tgttcggtgt tgttgatctc atctttaaag gtaatctcaa cgccagggca cagtactgct
     4501 ttggccttca gcacatgcgt caggcgtgaa acggaaaaac gcgggctgtc aaagaaggtt
     4561 tcatccggcc agaagtgcac gctggtacca gtattgcgtt taccgcaagt gccgacaacc
     4621 tgtaaatcct gcaccttttc gccattttca aaggcgatgt tataaacctg accatcgcgg
     4681 cgcacgttaa cttctacgcg cttcgacagg gcgttaacca ccgaaatccc cacgccatgc
     4741 aggccgccag agaactggta atttttgtta gagaatttac cgcctgcatg cagacggcaa
     4801 agaatcagtt caaccgccgg tacaccctct tccgggtgaa tatccaccgg catcccgcgc
     4861 ccatcgtcaa taacttctaa cgactggtca gcatgtaaaa taacgtccac gcgttttgcg
     4921 tgacccgcca gtgcttcatc cacactgtta tcaatgactt cttgccccaa atggttaggg
     4981 cgagtggtat cggtatacat ccccggacgg cggcgaaccg gctcaagccc ggtgagtacc
     5041 tcaatggcat cagcgttata agtttgcgtc atggtttaag ttagcaattc gagttgatcg
     5101 tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg aaataatctt cgaagcccgt
     5161 gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag gaagcgtagt acgccaccgc
     5221 ctggcggtaa tccagcactt catctcccgt ctgttgcagc agccagatca aatccggcgc
     5281 ttccagcggg tcaatctgca tgactttgag atcgtaaata tggcgtgact ctagcacata
     5341 ttgctgcccg gtgtaggggt tctcgttctg accgagatag tccgtcagca gttcaaacgg
     5401 gcgcaccgcc gggtttacca ccactgcggg cagcataaaa cattgtgaca accaggtggc
     5461 gtaatatccc cccagtgacg aaccgacaat acccagcgaa tcgccgccat gttccaggac
     5521 aatggattcc agcagctctg ccgcgtcgga aggatacggc ggcaactgcg gaatgatcat
     5581 ctcaacgtca gggtgatgtt ccgccagcca gttttttaac aagctcgctt ttgcagagcg
     5641 cggcgagctg ttgaaaccgt gtaaataaag aagcgtagac atcagtagcc ttctgaagcg
     5701 gtatcaggtt ggaaacgtgt gtccgccagg cgatgcacct cggtggtcag cgtgccatca
     5761 gcatgtaact cgagagtacg ccagccgggc gcgatagtat ccagcgtaaa gttggaacag
     5821 tgcggcttaa actgcacaca ggtcgacggc gttgccagca ggcggcgacc gttccagtcg
     5881 agatccagct cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg aaacttcgcc
     5941 agcacggtat ccagttcgcc cgcgttacgc aggctgtgtt gatcaagcca actacaaccc
     6001 gcaggtagcg gatgatgatg cagcagcagc aacgtatggc gctctggcgc atcagccaat
     6061 ttacgttcca gccactcaag ctgaaactcg cttagctcac cgtgcggcac gccaaacacc
     6121 tggctatcca gcaacaggat ttgccattgc tcaccaataa atacccgctt cgccggggag
     6181 atacccgcat cctgtaacgc gctgtacatc gcgggctgga aatcgtggtt gcccggcagc
     6241 cagacgcagg gcgcacgaaa acttgcgatg ccttcagcga aatgctgata ggccgcagag
     6301 gattgatcct gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg ctggtgtgga
     6361 cgaatcgcct ccagcaccgc ctggtagctt tcccaggtgt ttacccctaa cagggcttcg
     6421 tgcttttgtg caaacaggtg agtatcggta atttgtaaaa tcctgactct ggcctcacca
     6481 gccagaggaa gggttaacag gctttccaaa tggtgtcctt aggtttcacg acgctaataa
     6541 accggaatcg ccatcgctcc atgtgctaaa cagtatcgca accagtccgc taaaaactga
     6601 ttaatttgat gcttttcgtc gcgttgatgc aactttttat taggataatc ataccgcgct
     6661 ttgaagcgaa aaatctgctg gcttgaacac acttcagcca ccatcgcgtc atgatacaga
     6721 cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg ttctattgtc
     6781 accagggtag tgtatcgggt cgattccaca atcgtcagcc gatattgtgc gtttgccacc
     6841 tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg caattgtgaa
     6901 aagttcatct cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg cttcatttat
     6961 gcccactcat tttttaacgc ttgatgatgc agctgcagcc attgcaaagc gatgaccgac
     7021 gctgcgttgt cgattttccc ctcttctacc cactggtatg cctgttcccg gcttaccaca
     7081 tgaacgcgaa tatcttcgtt ttcatcagcc agaccgtgaa taccgcttgc ggtcgtggcg
     7141 tccacttcgc ccaccataat tgacgaacgc tcactggtgc cccccgggct tgccaggaaa
     7201 cttaacaccg gtttggtccg tttgactatc agccccgcct cttcaatcgc ttcgcgacgg
     7261 gcaacatctt ccacactttc accctcttca atcattccgg caaccatctc cagtagccaa
     7321 ggggtttcgc tagtgtcgta tgcggcaatt cgaatctgct caatcagcac aacttcatca
     7381 cgcactgggt caaagggtag caagactgcg gcgtgaccgc gctcaaaaat ttcccgccgt
     7441 acctcatgac tcatttgccc gttgaataga cgatgacgaa atctataaag atctaatgaa
     7501 aaaaagccgc gataaagtgt ttctcgtgca ataatttcta catcgttttt gccaaatgta
     7561 acgggcaggt tgtctggctt aagcattgtt aatgtcctgg cactaatagt gaattaaatg
     7621 tgaatttcag cgacgtttga ctgccgtttg agcagtcatg tgttaaattg aggcacatta
     7681 acgccctatg gcacgtaacg ccaacctttt gcggtagcgg cttctgctag aatccgcaat
     7741 aattttacag tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa atgaagaaat
     7801 tgctccccat tcttatcggc ctgagccttt ctgggttcag ttcgttgagc caggccgaga
     7861 acctgatgca agtttatcag caagcacgcc ttagtaaccc ggaattgcgt aagtctgccg
     7921 ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg cagtccatta ctgccacagc
     7981 taggtttagg tgcagattac acctatagca acggctaccg cgacgcgaac ggcatcaact
     8041 ctaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg tcgaaatggc
     8101 gtgcgttaac gctgcaggaa aaagcagcag ggattcagga cgtcacatat cagaccgatc
     8161 agcaaacctt gatcctcaac accgcgaccg cttatttcaa cgtgttgaat gctattgacg
     8221 ttctttccta tacacaggca caaaaagaag cgatctaccg tcaattagat caaaccaccc
     8281 aacgttttaa cgtgggcctg gtagcgatca ccgacgtgca gaacgcccgc gcgcagtacg
     8341 ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg gtagagcagc
     8401 tgcgccagat caccggtaac tactatccgg aactggcggc gctgaatgtc gaaaacttta
     8461 aaaccgacaa accacagccg gttaacgcgc tgctgaaaga agccgaaaaa cgcaacctgt
     8521 cgctgttaca ggcacgcttg agccaggacc tggcgcgcga gcaaattcgc caggcgcagg
     8581 atggtcactt accgactctg gatttaacgg cttctaccgg gatttctgac acctcttata
     8641 gcggttcgaa aacccgtggt gccgctggta cccagtatga cgatagcaat atgggccaga
     8701 acaaagttgg cctgagcttc tcgctgccga tttatcaggg cggaatggtt aactcgcagg
     8761 tgaaacaggc ccagtacaac tttgttggtg ccagcgagca actggaaagc gcgcatcgta
     8821 gcgtcgtgca aaccgtacgt tcctccttca acaacattaa tgcttctatc agtagcatta
     8881 acgcctacaa acaagccgta gtttccgctc aaagctcatt agacgcgatg gaagcgggct
     8941 actcggtcgg tacgcgtacc attgttgatg tgttggatgc gaccaccacg ctgtacaacg
     9001 ccaagcaaga gctggcgaat gcgcgttata actacctgat taatcagctg aatattaagt
     9061 cagccctggg tacgttgaac gagcaggatc tgctggcact gaacaatgcg ctgagcaaac
     9121 cggtttccac taatccggaa aacgttgccc cgcaaacgcc ggaacagaat gctattgctg
     9181 atggttatgc gcctgatagc ccggcacccg tcgttcagca aacatccgca cgcactacca
     9241 ccagtaacgg tcataaccct ttccgtaact gatgacgacg acggggcttc ggccccgtct
     9301 gaacgtaagg caacgtaaag atacgggtta tctgccgcat tcttccccct tctcgcttca
     9361 atttcgacca gccatcctct attctgatgg gtatttacca caggtcccgg aagacaaaaa
     9421 tgaaacggac aaaatccata cgccacgcat cgttccgcaa aaactggagc gcacgccatc
     9481 tgacaccagt cgctctcgcg gttgccactg tttttatgct ggctggctgt gaaaagagtg
     9541 atgaaacagt gtctctctat caaaatgctg acgactgttc agctgcaaac ccaggcaaaa
     9601 gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga agccgaacgt actgcgccga
     9661 aatacgccac ccgtgaagac tgtgttgctg aatttggtga aggtcagtgc cagcaagcac
     9721 cagcccaggc tggcatggca ccagaaaacc aggcgcaggc tcagcaatcc agcgggagtt
     9781 tctggatgcc gctgatggcc ggttacatga tgggtcgtct gatgggcggc ggtgcgggat
     9841 ttgcacagca gccgctgttc tcctcgaaaa acccagccag tccggcttac ggtaaatata
     9901 ccgacgcgac gggtaaaaac tatggcgcag cccagccagg ccgcaccatg accgtaccga
     9961 agacggcaat ggcaccaaaa ccggcgacca ccactaccgt tacccgtggc ggttttggtg
    10021 aatctgttgc caaacaaagc actatgcagc gtagcgcaac cggtacctct tctcgttcaa
    10081 tgggtggctg ataccgatgg aaagagtcag tattaccgag cgcccggact ggcgtgagaa
    10141 agcccacgaa tacggtttca attttcacac catgtacggc gagccgtact ggtgtgaaga
    10201 tgcttactac aagttgaccc tcgcccaggt tgaaaagctg gaagaagtca ccgccgaact
    10261 gcaccagatg tgcctgaaag tggtggaaaa agtgatcgcc agcgatgagc tgatgaccaa
    10321 attccgcata ccaaaacaca cctggagttt tgtgcgccag tcatggctga cgcaccagcc
    10381 atcgctttat tcgcgtcttg atctggcgtg ggatggcact ggcgaaccta aactgctgga
    10441 aaataacgcc gatacgccaa cgtcactata cgaggcggcg ttctttcagt ggatctggct
    10501 ggaagatcag cttaacgccg gtaacttgcc ggagggcagc gaccagttta acagtctgca
    10561 agaaaaactg atcgatcgct tcgttgagct gcgtgaacag tatggcttcc agttgctgca
    10621 tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt tgcaggactg
    10681 cgcgacggaa gctgaaatcg ctaccgagtt cctctacatt gacgatatcg ggttaggtga
    10741 aaaaggtcag ttcacggatt tacaggatca ggtaatttcc aacctgttca agctgtatcc
    10801 gtgggaattt atgttgcgtg agatgttttc caccaagctg gaggatgcag gcgtacgctg
    10861 gctggaaccg gcgtggaaga gcattatctc caacaaggca cttctaccgc tactgtggga
    10921 gatgttcccg aatcatccga acctgctgcc cgcttatttt gcggaagatg atcatccgca
    10981 aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg tgtcgatcat
    11041 tgagaacggc aaaaccattg aagcagcgga aggtccgtat ggcgaagaag ggatgattgt
    11101 tcagcaattc cacccgttac cgaaattcgg cgacagctat atgctgattg gtagctggct
    11161 ggtgaacgat caacccgccg gaattggcat tcgtgaagac cgtgcattga tcacccagga
    11221 tatgtctcgg ttttatccac atatttttgt tgagtaatca atgatgccgg atggcattcg
    11281 ccatccggta attgttaacc tatctgcacc gacagcatac ttaaactgcc catttctatc
    11341 ccatcgaccg gaatggtaat tggctcctgc ccatcccacg cacctaagac atacagcaac
    11401 ggcagatagt gctctggcgt tgggttcgat aacgcgccac cttcatggtc gagataattc
    11461 accagaggat gctgctctac cggcccttgc cacgtcagat tcgctttcac atactcatta
    11521 aacgacgtcg cccacggata cggtgaacta tcaccgtgcc acttcactgt gcgcaggtta
    11581 tgcaccacgt taccgctggc gaccaacatt attccttcat ctcgcagcgc cgcgagtttg
    11641 cgccccattt cgaaatgcca ggcggcaggt ttgctactgt cgatactcaa ctgcaccatc
    11701 gggatatcgg cgtcaggata catcttaatc agcacgcccc aggaaccgtg gtcaaagccc
    11761 caggcttctt tatccagcgt caccgggacc ggcgctaaca gctcaaccag acgctgtgcc
    11821 agcgcaggcg aacccggagc aggataatgc gtatcgtaca gcgcctgcgg gaagccacca
    11881 aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg ggtaaaccag
    11941 tgagccgaaa ccaccacaat cgcttgcggg cgtggcaatg tcatccccaa cttctgccag
    12001 ctgcgggtat acaaattatc ttccagcacg ttcatcggac taccgtgacc taaaaacaat
    12061 gctggcatac gtgttgaaga catgatgata tccttaacta aaggtgtcat tttgatatcc
    12121 tcacaatacg cttgttcggc ggagtaagaa cccggataac aatgatgatg atcatcagtt
    12181 attttgacga tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc agtacctctc
    12241 attctgacca tactggcggg ggcagccacg tttattggcg cgtttctcgg cgttctcggg
    12301 caaaaaccct cgaaccgctt actggcgttt tcgctaggat ttgcggcggg gatcatgttg
    12361 ctcatctcat taatggaaat gcttcctgcc gcactagcag ctgaaggaat gtcgcctgtg
    12421 ttgggttatg gaatgtttat cttcggtctg cttggctatt ttggcctgga ccgcatgttg
    12481 ccacatgcgc atccgcagga tttaatgcaa aaatcggtgc agccgttgcc aaaatcgatc
    12541 aagcgcacag ccattctgct cactctcggc atcagtctgc ataacttccc ggaagggatt
    12601 gccacctttg tcacggcgag cagcaacctg gagctgggat ttggcatcgc actggccgtc
    12661 gcgttgcaca atattcctga aggtctggcg gttgcaggcc cggtttatgc agcaacgggt
    12721 tctaaacgca cggcgattct gtgggcgggg atttctggac tggcagaaat tcttggtggt
    12781 gtgctggcgt ggttaatcct cggtagcatg atttccccgg tggtcatggc agcaatcatg
    12841 gcggcggtcg caggaattat ggtggcgctc tcggttgatg aattaatgcc gctcgccaaa
    12901 gagattgacc ctaataataa ccccagctat ggcgtactat gtggaatgtc agtgatggga
    12961 ttcagtttgg tgctgctaca aacggcggga attggttaaa agcaacatca gaagttgtcc
    13021 agcgcaggga caacttctgc tagcttcact aaagccttat tctacctttt cggctgtctc
    13081 ttctctcgta ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc tgtcctggcg
    13141 aaacgcccga caataacgtt tcaaatgcag gaaataaccc accacaacca gagcaatcaa
    13201 tacaatccag taccaggcga taaacatcct tttaccctta atatttaaca atactaaaca
    13261 tttagcgcat aaatttcaca tatccttttt cggatatatt catatggtcg cataataacg
    13321 gaacaattat atgattttga tcataatgaa aaagaatgcc tgatattaat gaaattttct
    13381 catagaaaga gaagcggaat taacatcttg catagcaggg aaaaataaaa accgaagcct
    13441 gctacaactt cggtttcttg attaaggcag taatttaagc agcggttttc agctggcttt
    13501 acgctcatgt gcctgacggt atgccaccag gtcttcaata gtcacgagcg ccatattgtg
    13561 tttattggca aactcaatac actctggtgc acgcgccatc gtgccatcgt cattagtcag
    13621 ctcacacagc acaccagccg gtttaaagcc tgccagcgtc atcagatcaa tagtcgcttc
    13681 agtatgaccg ccacgcgtca gtactccacc tgcctgcgcg cgaagtggga aaacgtggcc
    13741 cgggcgattc agatcagaag gttttgcgcc atcggcaatc gctgcgcgaa cggtcgtaat
    13801 acggtcagcg gcagaaacac cggtagtcac accttcagcc gcttcaatgg tcacggtaaa
    13861 accggtgcca taggcgctgg tgttattttc taccatcatt ggcagatcga gttgtttacg
    13921 gcgatcttca gtaatgcaca ggcaaacaat accgctaccg tggcgaatgg tcagcgccat
    13981 ctgctcaaca gtcatggttt ctgccgggaa gatcatatca ccttcgtttt cacggtcttc
    14041 atcatcaagc accattacac cgcgtccttc acgcagcgca gccagtgcat tttcaacacg
    14101 ttcgaaaggc gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa aaacctcact
    14161 aaaattatgg ttaccagaat cagggcagtc ttaggagtgg cggcatatag ccaaaataac
    14221 gtgagcgggt ccatgcccga cagaatcgtt actctctccc atccggactc taaccgtcgg
    14281 ccccggaatt acaccggatc tgctgtcctt tgagttcgca cccaaagcgc tcgcgggctt
    14341 tcaactgagt tgatttaccg ccggtgggga atttcgcccc gccctgagaa taagcggatt
    14401 cactataacg ctaatgatta gcggcagcaa cgcatagctt cacataattc tggtttatga
    14461 cttaccctta tcgcactaca atggcactca acacctatca gtacagggaa actacaatga
    14521 ttgacccgaa aaaaattgag caaatcgctc gccaggttca cgaatcaatg cctaaaggaa
    14581 tcagggagtt cggggaagat gtggagaaaa aaatccgcca aaccctacaa gcgcagctga
    14641 cgcgtctcga tctggtaagc cgcgaagaat tcgacgttca gacgcaagtc ctgttgcgga
    14701 ctcgtgaaaa actggcgtta ctggagcagc gcatcagtga actggaagcg cgcaataatt
    14761 cggtagcaga tcttcaatct cctccggcta tcccgcctat tgataaagca gaataaagca
    14821 acacaaaaac gggccagacg gcccgtaata tcagtgttct aattcaagtt ctctggtttg
    14881 catcatctgt tggcaatacc aggcataacg tgcgcaaaac caggttctat gttcttcatc
    14941 catgttacca gtaacggcca gaatatcgac cgggcgacct tctgcgtgca ttctggcaaa
    15001 actacgcgcc aggaaatcga aattatttaa agaattaaga ctatgatcca taagcacatc
    15061 ctccggttat cgcatacctt ttaaggccat atgctttttc tgatttcagt attccccaat
    15121 cagtgtagtg cctttaaaca taacgtgcgt aaatatattg tccccgatca cactttttag
    15181 tgaaaaataa aaaaccatat taaaagactt tctcttccag aacttctcta acatgtgtta
    15241 ttattaacaa agacaaatgt aagcaaggaa actatcccaa agtaaatatt gcgtcactaa
    15301 atggacattg gagtgacata tgattttatt cgccgactat aacacccctt atcttttcgc
    15361 tatttccttt gtactgttga tcggcctttt agaaatttta gcgcttatct gcggtcacat
    15421 gctctccggt gctcttgatg cacatcttga ccattatgat tccataacca caggtcatat
    15481 tagccaggca cttcattatc tcaatatcgg aagattgcca gctctcgtcg ttctctgctt
    15541 gctggctggt ttttttggtc ttattggcat cctgttgcag catgcctgca tcatggtctg
    15601 gcaatcacca ctgtcgaacc tgttcgtggt tcctgtcagt ttactgttta cgattattgc
    15661 ggtgcattat accggtaaaa tagttgcccc ctggattcca cgcgaccata gttcagctat
    15721 tacagaagaa gaatatattg gtagcatggc attaattacc ggtcatcaag ccacttcagg
    15781 taatccctgc gaaggaaaac tcaccgatca atttggccaa atacattatt tactactgga
    15841 gcctgaagaa ggaaaaattt tcactaaagg agacaaagtg ttaattattt gccgactttc
    15901 tgcaacgcga tatttggcgg aaaacaatcc ctggccccaa atattgtaat ttattaatca
    15961 aaaggaaatt taataatgga tgatattgtt aattctgtgc cctcctggat gtttaccgcg
    16021 attattgccg tatgcattct gtttattatt ggaattattt tcgccaggct ctatcgtcgc
    16081 gcttcggcag agcaagcttt tgttcgtact ggtttaggtg ggcaaaaagt ggtaatgagc
    16141 ggtggcgcaa tcgtgatgcc gatctttcat gaaataatcc ccatcaatat gaatactctg
    16201 aagctggaag tcagccgctc aaccattgat agcctgatta cgaaagatcg tatgcgcgtc
    16261 gatgtagtag tcgctttctt tgtgcgggta aaaccttcag tagaagggat tgccaccgct
    16321 gcccagacgc tggggcaacg caccctgtcg cctgaagact tacgtatgtt ggttgaagat
    16381 aaatttgtcg atgccctccg tgcaacagct gcgcaaatga ccatgcatga gttacaggat
    16441 acccgcgaga acttcgtgca gggagtacaa aataccgtag ctgaagatct gtcgaaaaac
    16501 ggcctggaac tggaaagcgt ttcacttacc aactttaacc agacctcgaa agaacatttc
    16561 aatcctaaca atgcctttga cgccgaaggt ttaaccaaac tgactcagga gacagagcgc
    16621 cgtcgccgcg agcgtaacga agttgaacag gatgtagaag ttgcggtgcg tgagaaaaac
    16681 cgtgatgcac tttcgcgcaa gttggagatt gaacaacaag aagcgtttat gacgcttgag
    16741 caggagcagc aggttaaaac ccgtaccgct gagcagaatg cgaaaattgc ggcttttgaa
    16801 gctgaacgtc gtcgtgaagc agagcagacg cgaattctgg ctgaacgaca gattcaggaa
    16861 acggaaatcg accgcgaaca ggccgtccgc tcaagaaagg ttgaggccga acgtgaagtt
    16921 cgcattaaag agatcgaaca gcagcaggtc accgaaatcg ctaaccagac gaaatcgatc
    16981 gctattgccg ccaaatcgga acaacagtcc caggcagaag cgcgtgctaa ccttgcactc
    17041 gcagaagcag taagcgccca acaaaacgta gaaaccactc gccagacagc cgaagccgat
    17101 cgtgccaagc aggtggcgct gattgccgca gcacaggatg cagaaaccaa agcggttgaa
    17161 ctgaccgtgc gggcgaaagc agaaaaagaa gccgcagaga tgcaggcggc ggcgatcgtt
    17221 gagttagccg aagctacgcg taaaaaaggc ctggcggaag cagaagcaca acgtgcgctg
    17281 aacgatgcta tcaacgtact ttctgacgaa caaaccagcc ttaaattcaa actggccttg
    17341 ttgcaggcgc tacctgcggt aatagagaaa tccgtggaac caatgaagtc cattgacggt
    17401 atcaagatta ttcaggtcga tggtctgaat cgtggtggta ctgcggggga tgcaaacacg
    17461 ggtaatgtgg ggggcggaaa cctggcggaa caagcattat cagccgcact ctcttaccgc
    17521 acacaggcac cactgattga ctccttgctc aatgaaattg gcgtttcagg cggctcactg
    17581 gctgcattga cttcaccctt aacctcaaca actcccgtcg ccgaaaacgt agaataaatt
    17641 ttcagctatg aacatccctg gcacaaaaag ccggggattt tttatatctg cgttccgcta
    17701 aaaggtgcaa atgatcaggc tgttgcagcg ttttgcgtga ctgctcgggg aaggcaaaac
    17761 tgcttcaggg aaagcgttgc gcgaggtccg gcgctcatca acaatcgggg agtcagcaaa
    17821 gggctgaaac gggaaagccc ctcccgagga aggggcctta cagaaggaaa gggttatgat
    17881 gaagctcgtc atcatactga ttgtgttgtt actcgtaagt ttcgcagctt attaacagcc
    17941 aatcagaggg gggagaaatc ctccctcacc cttgttcctt tactttaggt tgaaaaaaaa
    18001 tcagcgtcaa gaaggtttca ttaacacata tccctggccc aaaaagccgg ggatttttta
    18061 tatctgcgtt ccgctaaaag gtgcaaatgc tcaggacgtt gcagcgtttt gcgtgaccgc
    18121 tcggggaagg caaaactgct tcagggaaag cgttgcgcgg ggtccggcgc tcatcaacaa
    18181 tcggggagac agcaaggggc tgaaacggga aagcccctcc cgaggaaggg gccttataga
    18241 aggaaagggt tatgatgaag ctcgtcatca tactggttgt gttgttactg ttaagtttcc
    18301 cgacttacta acaactcatc agagggggga gaaatcctcc cttacccttg ttcctttact
    18361 ctaggttgaa aaaacaacag cgtcaatagg cctgccatgt acgaagcgag atctgtgaac
    18421 cgctttccgg ttagcctttt ttatcctgtt ggatcttctt gataatgtta gtggtcgagc
    18481 aaccgtcttc aaagttgagc accaacactt cgccgccgtt ggcccagact tctttactcc
    18541 cggcgatctc ttccggtttg tagtcaccgc ctttcaccag caggtctggc aggatcccgg
    18601 cgatcagacg ctgcggcgtg tcttcttcaa acgacactac ccagtcgacc gcttccagtg
    18661 cgcccagcac aatcatacgc tgttcgagtg ggtttaccgg gcgggaatcc cctttcagcc
    18721 gtttggtgga ggcatcgctg ttgacggcaa caatcaagcg gtcacccagc ttgcgggcat
    18781 ttgccagata agagacgtgt ccggcatgta ggatatcgaa gacaccgttg gtcattacca
    18841 ctttttcacc acgtttacgc gctgccgcta cagccagctt cagttcctct tcggtcatca
    18901 cgccaaagcc ggtatctgcg cgtccacgta cagcgttttc cagctcgatc ggcgaaaccg
    18961 tggaggttcc cagtttgccg accaccacgc cagccgccgc attggcaaag aagcaggctt
    19021 cttccagcga attacccgct gccagcgttg ccgccaggac gccaatcacc gtgtcgcccg
    19081 caccggtaac gtcatacact tcctgcgcct gagttggcat atgcagcggc gctttacccg
    19141 gttgcagcag cgacataccc tgttcggaac gggtcactaa cagagccgag agttcgtaat
    19201 cggcaatcag tttcatgccg cgctcaacaa tctcttcttc ggtcttacat ttaccgacaa
    19261 cagcttcaaa ttccgagaga ttcggcgtta acagcgtagc gccgcggtag cgctcaaaat
    19321 cggtaccttt tggatcaatc agcaccggaa cacccgcttt acgcgccagt tggatcatct
    19381 gctgtacgct tgccagcgca cctttggcgt agtcagaaag caccagcgcg ccaatcgaac
    19441 tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc aacaccttcg aaaccttctt
    19501 caaaatccag acggatcagc tgttggttgc gggaaagtac ccgtaatttg gtaatggtcg
    19561 gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac gtcggccaga gatttactca
    19621 gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag gcgtgcatta gcaccgagag
    19681 aagcgatatt catcgccacg ttagccgcgc cgcccggacg ttcttcgatg gtattcactt
    19741 taaccacggg caccggcgct tccggcgaga tacgactggt ggggccgtac cagtaacgat
    19801 ccagcatcac atcaccaacc accatcactc ctgcacgttc aaactctggc agcgttactt
    19861 tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat aataccatac ttcattcttc
    19921 caccagccac ttctgccagc ttgcccgcac cagttcacgc tctgcggtga agcaatcctc
    19981 cgacacatgg cccggcaatt cctgtaatgc cagatgatga agttcatcgc gaagcgtagt
    20041 gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata atgtcgtttt gcgccagtag
    20101 ttccagaata cgcacgttgt ctgaccagcg cgttaacttc ggtttttcat gagcgtagcg
    20161 caacaccaga tattgggtaa taaattcgat atcggtaatt cccccttcat cagctttgat
    20221 atcaaagcga tcgcgatgtt tattgccgag atgagcgcgc attttctcgc gcatttcccg
    20281 cacttccgtt tgcagagttt taccttcacg cggcagcgtc ataatctcgc gacgcactgc
    20341 gtcaaagtgc gcggtgagct gcggatcgcc gtacactaca cgcgcacgta cgagcgcctg
    20401 atgttcccac gtccaggcct cgtttttctg ataatcggca aatgcttctg cggatgtcac
    20461 cagcattccc gcggccccgg acggacgcag tcgagcatcc acttcataca aaatgccgga
    20521 agaagtacgc gtactgaaca gatgcataat gcgttgcgcc agacgcagat aaaactgtcg
    20581 cccgtcgatt gcccgctcgc cgtcagtcat tgcatccatc gggcaatcat ggaggaagat
    20641 caggtcaaga tcggaactgt agcccaactc ccagccgccc aacttaccgt agccgaccac
    20701 cgcaaaaccg cggccttcgc gttcgttcag gtgatttggt ttaccgtagc gggccaccat
    20761 ttgaacccac gcctgctgta cgacagcatc tatcatggct tccgccagcc aggttaagtg
    20821 atcgctcact ttcattaccg gcagcgtccc cgcaatatcc gctgccgcaa tgcgtaacag
    20881 ctgcgcctgt ttgaactggc gcagcgcctc aagctgctgt tcttcgtcat cttccggcac
    20941 gcgcagcaaa tactggcgca actcatcgcg gtaggcatcg gtcgccgtcg gctggtaaag
    21001 ggtgtttgga tcgagcaatt catccagcaa taatggataa cgcgccagct ggctggcaat
    21061 catcggcgac gcggcacaca gagaaatcaa atgtttaagc gccgcgggga attcactgag
    21121 caattctaaa taggtggtgc gggtaacaat ccccaccagc aaggcggtaa tgcgcgacag
    21181 cgtaacggca gcgtcttcac gcgcacagac atcacttagc agatgcggca tcagatggtc
    21241 gagcacctga cgtcctcgcg gcccgatggt gcgcttatcc agctctttgc ggaaatcagc
    21301 aatcagcgtt agcacctgtt tgcgatcatc ctcgctaaga tgcgccagca ctggcgtagt
    21361 gtcatcttcc tgcaacgcat cctgccacag ctcacgccac tgttccgaca gcgactcttc
    21421 ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac acccggcgca cattggtcat
    21481 atgtgcggtc agcgccccgg tcaattgcgg ccagtcagca aagtccatcg cccacgccag
    21541 ccgcgcacga ttaagctcat cagaaggaag cgtctgggtt tgttcgtcgt taatgctttg
    21601 cagcaggttt tccagacgcc gcaggaacag atacgccact cgcaattgtt cagcatcgtt
    21661 ttcagaaagc agatgcagcg cagcaatggc gctgagcgtt ggcagtaaag agcgcgattg
    21721 cagcgacggt tcgcgcccgc cgcgaatgag ctggaatacc tggacgataa attcaatttc
    21781 acgaatgcca cccgcgccaa gtttgatatt gtcggtcaaa ccgcgtcgac gcacttcacg
    21841 ggcaatcatt cctttcatgt tgcgcagcga ctggatgacg ctgaaatcga tgtaacggcg
    21901 gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca tagacgccgt cgctatcgcc
    21961 cataatacgc gccttgacca tcgcgtaacg ctcccagtcg cgcccctgct cctggtaata
    22021 atcttccaac gcggcaaagc tcagcaccag agggccactt tcgccaaacg gacgcagtcg
    22081 catatccacg cgatagacga agccatcctg cgttggttga tccagcactt taatcagccg
    22141 ctgccccatg cgggtaaaaa actgcgcatt atccagttcc cggcgtccac cctgcgtaca
    22201 accatgttcc ggccaggcaa aaatcagatc gatatctgag gagaaattca gttccccgcc
    22261 gcccagctta cccatgccta aaatcagcag cggttgcgct tcgccctgcg cattgcacgg
    22321 cgttccccac tcgcggcagc aggcgtcata tagccagtca cgcgccgcaa caatcagcgt
    22381 ctccgccaga tagctgagct gctgcaatat gctctcttca gtaaccagtg ccagcgtttg
    22441 cgcccaggcg atacgtacca tgatgcgccg acggaacagc cgcagctcgc gcattaaccc
    22501 ggcttcgtca ctcacattac tgagcgcctc ctgcaaccat gcaacgtaat gctgccattc
    22561 gtcggcctgc ggcggatggc tttccagttc cgtcagccac tctggatgcg cacttatgct
    22621 gtcctgcaca aaatcactga aagtaagtac tgacttcgcc tgtgcactaa gtgattcctc
    22681 ggctaaaggc tctggcagcc gctcaacaac ggtctgccag tactgctgta acggtgaaga
    22741 gagcggcttc ataagcgatt ttatccttgc ctgattaacg ttttccgctg tgcaaccaga
    22801 acggttcctg attgtttgcc tcattacgga aatgttcaat ttcgatgcgt tgcccggtcg
    22861 caatagcgtg atgcagcccc tgccagttct ccagccaggc ttgcgcgacg acaggatcat
    22921 agtaacccgc cagcaacagt attgagtcaa tatcacgcgt caggcgtggc aactggtcac
    22981 ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag ttcagcggca tggcgggaaa
    23041 gatggatatc ggcaaagcgt ttgaaggagt cgctgatttt gccctgcgct ttggcatcta
    23101 aaaatggctg ccatgctttg cttaccaacc attcggtcaa cgccagcttc gccattgccg
    23161 tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga agcaatggtc gcttcgcatt
    23221 gggtcagcaa atcacgtaag tgagtgctcg ctttacgcgg cacaatacca ccgaacagca
    23281 tcagcgtatg acggaccagg ctaatggctg ccagcacctg ttctttcgcc gcatcgttgc
    23341 cgcgtaccca cagttcttca tgatactgcc attgcgctaa cgccagctcg agcgccgctt
    23401 ccagcccctg ttccacatcg gcttttgccg caacatgcaa aatggtggtc ggtttgattt
    23461 cacgcgccgg attgccctgc gccagatgat agccacgcgc cgctttgctc aggctgccct
    23521 ggcgtaatcc gatttgcgat accagttggt tcgccagttt cagcaccgcg cgcgtgtcgc
    23581 cgctaagcag ctccagttcc agctcacaga taggttcagc aaattccccc gctttcacct
    23641 caccctgatc gagagcgatt tcaattcggc tgccatcgac cgccaccagc catttttcgc
    23701 gataaaaatc ggtgctgaac agcggttgta cgcgggaagc gagatcggcg ggcaattcgc
    23761 cgttcggcca gacttccgtc ggtaactgcg ccaggtcgag cgtcggttcg ctcaacgcca
    23821 cgttatattc cgggcgctgg tgtaagccgc ctgtcactct tcctgcaact ttcatggtca
    23881 tctcatagcg accgttttcg ccacgaatac gtaagcccat atcgtgccca cgcagccagt
    23941 tatccggcgt ttcgtagtaa atattcagta actgcacggg gtcatggtgc tcgccgccca
    24001 gcgtattgag atggtcacgc aacgcctcaa cggcgctgtg attaacaata aactttaatt
    24061 cgatttcctg agccatggcc tttacttatc ggttatgtca cataagtgac gatgaacggc
    24121 gaacttaatg cgatcttttt tgtcagtaga tagtattttg cgccaaattg ccatgcaacg
    24181 ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga cctgacacag aggattccga
    24241 ttgatgtcga atgctttgcg tagcgacact gatgccacta ctatcgttcc actttccatg
    24301 acaataacga cagcctgatg ccaaaattac gcctgatcgg attaacttta ctcgcactta
    24361 gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt ttctgacgaa ctgaatacct
    24421 gggtccgtag cggtccggga gatcattatc gcctcgtggg cacggttaac gccggcgagg
    24481 aagtgacctt attacaaact gacgccaaca ccaattatgc ccaggtgaaa gacagctctg
    24541 gccgtaccgc ctggatcccg ttgaaacaac ttagcactga accaagcctg cgctcccgtg
    24601 tgccagatct ggaaaatcag gtcaaaactc tgaccgataa actcaccaat atcgataaca
    24661 cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc gcagagcgac agcgtgatca
    24721 acgggttaaa agaagaaaat cagaaactga aaaacgagct gattgtcgcg cagaaaaagg
    24781 tcgatgccgc cagcgtacag ctggatgaca aacagcgcac catcatcatg caatggttta
    24841 tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg tctggtactg ccgcacctga
    24901 tcccaagccg caaacgcaaa gatcgctgga tgaactaaat cgccttcttc gttgcactgt
    24961 cttattatta ggaattgtgt atttgacgaa gggatgatgg cgtgaagatt tatctggtcg
    25021 gtggtgctgt tcgggatgca ttgttagggc taccggtcaa agacagagat tgggtagtgg
    25081 tcggcagtac gccacaggag atgctcgacg cgggctacca gcaggtaggc cgcgattttc
    25141 ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct ggcacgtacc gaacggaaat
    25201 ccggctccgg ttacaccggt tttacctgct atgccgcacc ggatgtcacg ctggaagatg
    25261 atcttaagcg tcgcgatctg accattaatg cgctggccca ggacgataac ggcgagatta
    25321 tcgacccgta caacggtctg ggcgatctgc aaaatcgtct gttgcgccat gtttccccgg
    25381 cttttggcga agatccgtta cgcgtattgc gcgtggcgcg ttttgctgcg cgttatgccc
    25441 acctcggttt tcgtattgcc gatgaaactc tggcgttgat gcgcgagatg acccatgcgg
    25501 gtgaactgga acacctgacg cctgaacggg tatggaaaga gacagaaaac gccctcacca
    25561 cccgcaatcc acaggtgttc ttccaggtac tgcgcgattg cggcgcactg cgcgttttat
    25621 tcccggaaat tgacgccctg tttggtgttc cggcccctgc caggtggcat ccggaaatcg
    25681 acacgggtat tcatacctta atgacgctct caatggcggc gatgctgagt ccgcaggtcg
    25741 atgtccgttt cgcaacttta tgccacgatc tcggtaaagg actgacgccg ccagaactct
    25801 ggccgcgtca tcatggtcat ggcccggcgg gtgttaagtt agtggaacaa ctatgccagc
    25861 gtctgcgcgt gccaaatgaa attcgcgatt tagccagact ggtggctgag tttcacgatc
    25921 tcatccacac cttcccaatg ctgaacccaa aaaccatcgt caaattattt gattccatcg
    25981 atgcctggcg taaaccgcag cgtgtcgagc aactggcgct gaccagcgag gctgacgtgc
    26041 gcggcagaac cggttttgaa tcagcggact acccgcaagg ccgttggttg cgcgaagcct
    26101 gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga agcgggattt aaaggcgtgg
    26161 agattcgcga ggagctgacc cgacggcgga ttgcggcggt agccagctgg aaggaacaac
    26221 gttgcccaaa gcctgaatga gtattgggca ttttgccgga tgcggcgtaa acgccttatc
    26281 cggctacatg tcaacgacag ttgtaggcct gataagacgc gcaagcgtcg catcaggcgt
    26341 tgattgccgg atgcggcgtg aacgccttat tcggcctacg caatcgtgga caacgccagg
    26401 catccgacac tattcctcaa tcaaaagaac acgacataca ccgcagccgc cacaataaag
    26461 cgataaatgg cgaacgggat aaacgaaatg cgcttaatca attgcaggaa ggttttaatc
    26521 gctatcagcg ccaccacaaa agcggtgata aacccaacgg caaacatcgg gatatcgccg
    26581 gttgtcagga agccccagct tttgtagaga tcgagcgccg ttgcgcccat catcatcggc
    26641 accgccagca ggaacgaaaa ctcggaagca gcgtaacggc tcacccccat cagcatccca
    26701 cctgaaatgg tcgccccgga acgggagaaa cccggccaca gcgccagaca ctggaaacag
    26761 ccaatcataa acgcctgacg ataggtcata tcatcaagac ccggcgcacg cggctctttc
    26821 ggcttcaggc attcggcggc aatcagcaac aaaccgccaa cgaccagcgc atacatcaca
    26881 tttatcgggt taaacaatga cttaatcgtg tcgtggaaca acagccccag taccaccgcc
    26941 ggaatcatcc ccagcaaaat gtggatcagc gttaaacgac ctttgctttc tccttcgtgc
    27001 tgcaacgggc ggccaaagtg gatgccaatc aggccaaaca gacgccgcca gaacatcact
    27061 actaccgcca gaattgatcc taactggatc acaacttcaa aggttttcgc cgtgtcgccc
    27121 tcaaacccca acaagtgacc gacaataatc atatggcccg tgctggatac cggcagaaat
    27181 tctgtcaatc cttcgaccac acccaatatt gccgctatca gcagcgagtg catatcgctc
    27241 atcaataaac ccctaaatta ttaaaatgta ccgcttgtcc gaactactgc agtatgacca
    27301 ggttataacc gtttggttta acagctgtaa aattaattat tttctttcag attattgcca
    27361 cgctcaatga ttacgccaac attcgccgcc cgcgccactg cgcctggctt gctgagtttg
    27421 atacgcaccc acggcgagtt gaagcgtgct aacagcagct ccgccacctc ttcagcaacg
    27481 cgttccacca gcgcaaaacg cgccccctcg acgtggctga ccaccgtttc tgcaatgtca
    27541 gcgtaactga ggcaatccgc cacatcatca cttttcgccg ctttacggtt atcccacgcc
    27601 atttcgatat cgaacactaa cttctgttcg atggtctgtt cccagtcgta aacaccaata
    27661 gtggtgatta ccgaaagttg ctctataaat acaatatcca tcacgtcctg cctgcttttt
    27721 ggctaaccgg ataccacttc cggcgaaatg tgcgtattat ccacagattc atcgttgaac
    27781 acgaattttc aaaacggaac agcttatgag tgcaatcgcg cctggaatga tcctcatcgc
    27841 gtacctctgc ggctccattt ccagtgccat tctggtttgc cgcttgtgtg ggctgcccga
    27901 tccgcgaacc agcggctccg gcaatccagg cgcaaccaat gtgttacgta tcggtggcaa
    27961 gggagcagcc gtagcagtac tgattttcga cgttctgaaa ggaatgttgc ccgtctgggg
    28021 cgcgtatgaa ttaggtgtca gccccttctg gctaggctta attgccatcg ccgcctgtct
    28081 tggacacatc tggcccgttt tcttcggatt taaaggagga aaaggcgttg ctaccgcttt
    28141 tggtgccatc gcacccattg gctgggatct caccggagta atggcgggaa cctggttact
    28201 gaccgtgcta ttgagcggat actcgtcgct gggagcgatt gtcagtgcac tgattgctcc
    28261 gttttatgtc tggtggttta agccacaatt caccttcccg gtttcgatgc tctcttgcct
    28321 gatcctgctg cgtcatcatg acaacatcca gcgtctgtgg cgtcgtcagg agacaaaaat
    28381 ctggacgaaa ttcaaaagaa agcgcgaaaa ggatcccgag tgatttctgg tggatctaca
    28441 tgacctgata gccttcatcg ggcttgccca gccgttgctg gcaccatgcc gccagaaatt
    28501 ccacgcagac acgtaatttc atgctgcgat agagcggctc ccggtaaaca gcccagatat
    28561 tggcgctttg tgcatactcc ggcaatactt gcaccagttt gccactctcc agaaacggca
    28621 acacatccca ctcggaacgc agcataatcc ctttgccctc cagcgcccat tgcagcacaa
    28681 tctcgccgct attggaggaa agatgcccgc ttaccttcac cgattttttc tcctgaccgt
    28741 tccccaactc ccatattccg tgggtcatat cgcgctcttt ggtcaccaga cagtcatgac
    28801 gacttaattc ctgtaatgat tgtggctgcg gatatttttg cagatactca ggtgctgcgc
    28861 ataatattct ttt
//
LOCUS       DAZUCE010000038        28636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZUCE010000038 DAZUCE010000000
VERSION     DAZUCE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.038"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            111..773
                     /gene="acrS"
                     /locus_tag="ACHY03_001458"
     CDS             111..773
                     /gene="acrS"
                     /locus_tag="ACHY03_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFS3904705.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VI"
     gene            complement(776..955)
                     /locus_tag="ACHY03_001459"
     CDS             complement(776..955)
                     /locus_tag="ACHY03_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFS3904706.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(1039..1923)
                     /gene="yhdJ"
                     /locus_tag="ACHY03_001460"
     CDS             complement(1039..1923)
                     /gene="yhdJ"
                     /locus_tag="ACHY03_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFS3904707.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(2009..2305)
                     /gene="fis"
                     /locus_tag="ACHY03_001461"
     CDS             complement(2009..2305)
                     /gene="fis"
                     /locus_tag="ACHY03_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFS3904708.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2331..3296)
                     /gene="dusB"
                     /locus_tag="ACHY03_001462"
     CDS             complement(2331..3296)
                     /gene="dusB"
                     /locus_tag="ACHY03_001462"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFS3904709.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3625..4506)
                     /gene="prmA"
                     /locus_tag="ACHY03_001463"
     CDS             complement(3625..4506)
                     /gene="prmA"
                     /locus_tag="ACHY03_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFS3904710.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4518..5969)
                     /gene="panF"
                     /locus_tag="ACHY03_001464"
     CDS             complement(4518..5969)
                     /gene="panF"
                     /locus_tag="ACHY03_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFS3904711.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5959..6201)
                     /locus_tag="ACHY03_001465"
     CDS             complement(5959..6201)
                     /locus_tag="ACHY03_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFS3904712.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCCAMVKFIYRDIPLEDDDAA"
     gene            complement(6310..7659)
                     /gene="accC"
                     /locus_tag="ACHY03_001466"
     CDS             complement(6310..7659)
                     /gene="accC"
                     /locus_tag="ACHY03_001466"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFS3904713.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7670..8140)
                     /gene="accB"
                     /locus_tag="ACHY03_001467"
     CDS             complement(7670..8140)
                     /gene="accB"
                     /locus_tag="ACHY03_001467"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFS3904714.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9006..9836)
                     /locus_tag="ACHY03_001468"
     CDS             complement(9006..9836)
                     /locus_tag="ACHY03_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904715.1"
                     /translation="MKSLFNRLTGKAVSRTAFVEHLGHEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLEEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AINNLKQILPEVSAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGGLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTEQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(9873..10847)
                     /locus_tag="ACHY03_001469"
     CDS             complement(9873..10847)
                     /locus_tag="ACHY03_001469"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFS3904716.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVCTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10999..12939
                     /gene="csrD"
                     /locus_tag="ACHY03_001470"
     CDS             10999..12939
                     /gene="csrD"
                     /locus_tag="ACHY03_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFS3904717.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13244..14287
                     /gene="mreB"
                     /locus_tag="ACHY03_001471"
     CDS             13244..14287
                     /gene="mreB"
                     /locus_tag="ACHY03_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFS3904718.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            14353..15456
                     /gene="mreC"
                     /locus_tag="ACHY03_001472"
     CDS             14353..15456
                     /gene="mreC"
                     /locus_tag="ACHY03_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFS3904719.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            15456..15944
                     /gene="mreD"
                     /locus_tag="ACHY03_001473"
     CDS             15456..15944
                     /gene="mreD"
                     /locus_tag="ACHY03_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFS3904720.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15953..16546
                     /gene="yhdE"
                     /locus_tag="ACHY03_001474"
     CDS             15953..16546
                     /gene="yhdE"
                     /locus_tag="ACHY03_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFS3904721.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            16536..18005
                     /gene="rng"
                     /locus_tag="ACHY03_001475"
     CDS             16536..18005
                     /gene="rng"
                     /locus_tag="ACHY03_001475"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFS3904722.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            18073..21873
                     /gene="yhdP"
                     /locus_tag="ACHY03_001476"
     CDS             18073..21873
                     /gene="yhdP"
                     /locus_tag="ACHY03_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFS3904723.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRVELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGQVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSVPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDITISGNTLTLTNGLI
                     DTGFSRLTADGEWINNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            22029..23474
                     /gene="tldD"
                     /locus_tag="ACHY03_001477"
     CDS             22029..23474
                     /gene="tldD"
                     /locus_tag="ACHY03_001477"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFS3904724.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(23602..24531)
                     /gene="aaeR"
                     /locus_tag="ACHY03_001478"
     CDS             complement(23602..24531)
                     /gene="aaeR"
                     /locus_tag="ACHY03_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFS3904725.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24714..24917
                     /gene="aaeX"
                     /locus_tag="ACHY03_001479"
     CDS             24714..24917
                     /gene="aaeX"
                     /locus_tag="ACHY03_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFS3904726.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24925..25857
                     /gene="aaeA"
                     /locus_tag="ACHY03_001480"
     CDS             24925..25857
                     /gene="aaeA"
                     /locus_tag="ACHY03_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFS3904727.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVNDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25863..27830
                     /gene="aaeB"
                     /locus_tag="ACHY03_001481"
     CDS             25863..27830
                     /gene="aaeB"
                     /locus_tag="ACHY03_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFS3904728.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27922..28194
                     /locus_tag="ACHY03_001482"
     CDS             27922..28194
                     /locus_tag="ACHY03_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFS3904729.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQIFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(28250..28513)
                     /gene="yhcN"
                     /locus_tag="ACHY03_001483"
     CDS             complement(28250..28513)
                     /gene="yhcN"
                     /locus_tag="ACHY03_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFS3904730.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6892 a         7144 c         7582 g         7018 t
ORIGIN      
        1 ttatagcaac tcaatgttag cctctcgtat aaatacacat taggtgatag attaaccttc
       61 gctattttct cactccgtgt cgaatatatt tatttcctga ataattaatc atggcaaaaa
      121 gaaccaaagc cgaagctctg aagacccggc aagaactgat tgaaactgcc atcgcccagt
      181 ttgcgcagca tggcgtaagc aagacgacgc tcaacgacat tgccgacgcc gctaacgtta
      241 cgcgtggcgc tatctactgg cacttcgaaa acaagactca actgtttaat gagatgtggt
      301 tgcaacagcc ttcattgcgg gagttaatcc aggaacactt gacggctgga ttagagcatg
      361 acccgtttca acaattgcgt gaaaaattga ttgtcggctt gcaatatatt gccaaaattc
      421 cccgccagca ggcgttgctg aaaatcttat atcacaaatg tgaatttaat gatgagatgc
      481 tggccgaggg agtgatacgc gaaaagatgg gctttaatcc gcagactctc cgcgaagtat
      541 tgcaggcgtg tcagcaacaa ggttgtgtag caaataacct cgatttagat gttgtgatga
      601 ttattattga tggtgccttc agcggaattg ttcaaaactg gttaatgaat atggcgggtt
      661 atgatcttta taaacaagcc cccgctctgg tcgataacgt attaagaatg ttcatgccag
      721 atgaaaacat aacgaaatta attcatcaaa cgaatgaatt aagtgtcata taaattcaca
      781 ttctgtcctg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
      841 tattgcggca tttgtctgtt tctgttgcca gcagttcaat ccactgcatc agtaaagtat
      901 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      961 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
     1021 accaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccgact
     1081 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcagagta
     1141 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
     1201 accaatgaat tttcgcccgc tggagacggc tacggcaccg gtagtaaagc taccggcaaa
     1261 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
     1321 ttccggtttt tgcgtcgggt ggttttcata ttcatccatc aaataacgca cgcgcggaaa
     1381 atcccagacg ttacccggta ctttttgatg attgtaaggc tgtggaggat ttttgcgata
     1441 atcgatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
     1501 cgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcatacatgg aaccgtagtg
     1561 ttttttcgcc tgcactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
     1621 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca tgatgtacat
     1681 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
     1741 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1801 tgggtcggca aagatcagat cgacgctttc agtaggtagc tttttaagtt cggcaagggc
     1861 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1921 catggtcact ccctttgtga cacctataaa aaaggcgctt ccccatgccg agtagcgcct
     1981 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     2041 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     2101 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     2161 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     2221 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     2281 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     2341 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     2401 gtgcgccgaa actggtcatt gggagcgtgt tcctggagat accaggaaac gtgtttacgt
     2461 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     2521 agcaagcgct taacctctgc caaaggcagc gggggcagca actccccagt gtccagataa
     2581 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     2641 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     2701 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     2761 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2821 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2881 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2941 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     3001 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     3061 atttctttcg gatcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     3121 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     3181 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     3241 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     3301 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     3361 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     3421 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     3481 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     3541 tttgcgtaat taatcggcaa atttacgtgc cttttgtgac cttgctcgca cttcgcccta
     3601 cgtcacccta cggcgatgcg aaggttaatt cttacgaccg gtaatgcggc accactcttc
     3661 tttttccacg accgggtcca gtgcgaagct gtcggcataa gcttcgcaaa cgctctccgc
     3721 ctggcttgcc agaataccag aaaggcccag taagccgcct gaaactggca ggacgctaat
     3781 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3841 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cgccattacg
     3901 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3961 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     4021 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacaaag aggtggttgg
     4081 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     4141 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     4201 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcgattt tatgcgcgaa
     4261 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     4321 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     4381 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     4441 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     4501 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     4561 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     4621 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     4681 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     4741 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4801 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4861 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4921 tctttaatga tcgtagcgga actttgtagc aattgggcgt taattgtcga catgatcgca
     4981 gccataggtg cagccagaaa gatcccggca gcaaacggtg gcagcacttt taccattaac
     5041 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     5101 gccaggtgca taccgaacat caggattgcg actacaatcg taccgatgat gatcccccgg
     5161 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggcctata
     5221 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     5281 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     5341 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     5401 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     5461 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     5521 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     5581 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     5641 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     5701 gaaagccaga ctgcaggaag ctgaatcatc gccagcaata cccagccaag gccgtatttg
     5761 taagcagctc caggcccgcc gataaacgaa ctggcactga tataagtcgc ggtgagcgtc
     5821 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5881 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5941 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     6001 aaatttcacc atcgcacagc acagtccaat aaacagcagc ggcgtcagga tgcaggccat
     6061 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcggctac
     6121 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     6181 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     6241 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     6301 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     6361 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tagttttgat
     6421 accgtcgatg atcagctcct gcagcgcatt tttcatgcga gcaatcgcca cgtcacggtt
     6481 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     6541 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     6601 tttgcccggg cttggcagga aggtgttcgg atcttcggcg ttgatacggc attccaccgc
     6661 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     6721 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6781 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6841 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6901 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6961 ctgcatggag cagtcacgtt ccgccagata gatagcgttg ccctgaccgt cagccagtac
     7021 ctgaatctcg acgtggcgag gattttccag gtatttctcc atgtaaacca tatcgttgct
     7081 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     7141 gcgcactacg cgcataccgc gaccaccgcc gccgccggag gctttgataa tcaccggata
     7201 accaatgcgt ttagcaatgg cacggttttt atccatatcg tcgcccagcg ggccgtcaga
     7261 acctggtaca caagggacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     7321 catcaggcga atggtttctg ctttcgggcc aatgaagata aagccggagc gttcaacctg
     7381 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     7441 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taacttttta ctgacggagc
     7501 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgcgct
     7561 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     7621 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     7681 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     7741 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7801 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7861 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7921 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7981 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     8041 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     8101 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     8161 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     8221 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     8281 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     8341 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     8401 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     8461 gaagataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     8521 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     8581 ctgtaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     8641 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     8701 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     8761 ccgactgaca atgctcagca ttaaatacgt tcgagtggtt aatttgtagg caaataaaat
     8821 cagattattc cccccatttt gcagaaggta aactgttttg tcaggtggca gtactgctat
     8881 ctgatgcatt tagaatttgc cgcgagacaa taaaaaagcc gattatgtag caaaatcggc
     8941 ttcggtgagt ttgcagtttt catcacacac tccgacaagc tttttcaagc atgacggagg
     9001 ttgcgttaat gcaaggtttc tacccaggga taaaagtgac cgttacggcg gatgaatatc
     9061 tgtgttgtca gaggatggtc atcctcacgg aagcattgtt ctgttaactg tcgtaatgcg
     9121 ctgagatttg ctggctgatc attgtgggtg aaaaacagtt ttcctcggga gggaacagtc
     9181 actaacaggc cgccgcattc aggatcgggt gattgcaccc aaagatcgtc aatgagcatc
     9241 agactgggct cataagtacc cccagcagag ataacggcaa cttttccatc gtttgcgcct
     9301 gtatcaacga tttcagccga aacttccggc aatatttgct tcaaattatt gatggcttgt
     9361 tcgcggtcaa tggtcacttc gtagcgcatc agatagcgca tggtttcgct accatcttca
     9421 gcaaacaaga taattaactc atcgttaaag tcttcccagg caaaatccag cgaggcgttg
     9481 aggacctctg gatcagactc atactggcta agctgttggc gaacggtatc cagccaggct
     9541 ttttctttaa tcaccggaac aatacagcgc ccggtcggca gcatttctgc aagctgtttc
     9601 agcatatctg cagccacttc ttccagcggg tctggtgcgg cttgataacg tttgtaggca
     9661 tcatcaaggg tgatttccac cgtttggtga cccgtatcaa tgcgcatcaa tttatggtca
     9721 gtcgaaatca tgactttcca gtttggatga tgttgtacaa cttcatgacc aagatgttcg
     9781 acgaaagctg tccggctaac cgcttttccc gttaatcgat tgaataatga tttcaaaatg
     9841 agttccctct cttattaatc cttgctaaat ggttagttaa ccttcaccag cgtgcgaccc
     9901 tggatctggt tattaatgat ggcctcggca aagttcggtg cctctgacag agatatctct
     9961 tttgccgcct gggtatagaa tgattccggt aaatcggcga ccagtcgctg ccaggcttgt
    10021 gcgcggcgtt ctggtggcgt cattactgaa tccacccctt gcaaacggac attacgcaga
    10081 ataaatggca tgaccgtggt tggcagagta aaaccacccg ccagaccaca ggccgccacg
    10141 cagccgccgt aattcatttg cgccagcact tttgccagca ctttgtcgcc aacagtgtca
    10201 attgccccag cccagacttg tttttccaga ggacgggatt cggcaaactc atcacgaggg
    10261 agaatacggc tggcacctaa acttttcaga tattcatggg tactttcgcg accggaaacg
    10321 gcaacgacct gataacccaa cttatgcagc agcgccacgg cggtactgcc gacgccacca
    10381 ctggcacccg tcacgacaat ctccccgtcc tgcgggcgaa caccggcatc ttccagcgcc
    10441 atcacacaca gcatggcggt aaaaccggca gtaccgataa tcattgcttt acgcgcgtcc
    10501 agcccttgcg gcatggcaac cagccagtca cctttcactc gcgcctgctc cgccagccca
    10561 ccccagtggt tttcaccaac gccccagcca gtgagtaaca cctcctgacc ggcatgaaaa
    10621 cgcggatctt cgctggtgca tacagttccg gcaaaatcga tcccaggaat catcggaaaa
    10681 ttacggatga tttttccctt accggtaatc gccagcgcat ctttatagtt caggctcgac
    10741 cagtgaacat cgaccgtgac atcgccctcc ggcaggcgac tttcgtccag agtctgtact
    10801 gatgcgagag ttttgccgtc ctgctgttct aaaagtaacg cctgcataag tggtcctcat
    10861 gtgcatgatg gattggaaaa taatttctga agactatact cgctaatgga aaagcaaagc
    10921 cgatgaagcg caataaattg cgagataaat ctgatttgct agtatgcccg cttcctcact
    10981 atcggagtta acacaaggat gagattaacg acgaaatttt cggcctttgt tacgctgctc
    11041 accgggttaa caatttttgt gactttgctg ggctgttcgc taagtttcta caacgccatt
    11101 cagtataagt ttagtcatcg cgttcaggcg gtggcgacgg cgattgatac ccaccttgtg
    11161 tcgaatgact tcagcgcatt aaggccacaa attaccgaat taatgatgtc ggcagatatc
    11221 gttcgtgtag acctgctcca tggtgataag caggtttata ccctggccag aaatggtagt
    11281 tatcgtccgg ttggcaccag cgatctgttt cgtgaactga gcgttccgtt gataaagcat
    11341 ccggggatgt cgttgcgtct ggtttatcag gatccgatgg gcaactattt ccattcgttg
    11401 atgaccaccg cgccgctcac aggggcgatt ggctttatca ttcttatgct ctttctggcg
    11461 gtacgctggt tacaacggca acttgccggg caagaattgc tggaaacccg ggctactcgt
    11521 atcttaaacg gtgagcgtgg ctctaatgtg ttgggaacca tctatgaatg gccgcccaga
    11581 accagcagtg cgctggatac gctgcttcgt gaaattcaga acgcacgcga acaacacagc
    11641 cgtcttgata cgctgatccg ctcttatgcc gcccaggacg tgaaaaccgg cctcaataac
    11701 cgactctttt tcgataatca gttagcaacg ttactggaag atcaggagaa agtaggtacc
    11761 cacgggatcg tgatgatgat tcgtctgccg gatttcaata tgttgagcga tacctggggg
    11821 cacagccaag ttgaagaaca gttcttcact ctaacgaatc tgctgtcgac atttatgatg
    11881 cgctaccctg gcgcactgct ggcgcgttac caccgcagtg attttgctgc gctgttaccg
    11941 caccggacgt taaaagaggc agagagcatc gccggtcagt taatcaaagc cgtcgatacc
    12001 ttgccgaaca ataaaatgct cgatcgcgac gatatgatcc acattggtat ctgcgcctgg
    12061 cgtagtggtc aggataccga gcaggtaatg gaacatgcag agtctgccac gcgtaatgcg
    12121 ggattgcagg gcggcaatag ctgggctatt tacgatgact cgttgcctga aaaaggacgc
    12181 ggtaatgttc gctggcgtac gcttatcgag caaatgctca gtcgcggcgg cccgcgcctt
    12241 tatcaaaaac cggcggttac tcgcgaaggt caggttcatc atcgcgaact catgtgccgc
    12301 atcttcgatg gtaatgaaga ggttagctcg gcggagtata tgccgatggt cttgcagttt
    12361 ggcttatcgg aagagtatga ccgtctgcaa atcagccgtc ttattccact attgcgttac
    12421 tggccagagg aaaatctggc gattcaggtt accgttgagt cgctgattcg cccgcgtttt
    12481 cagcgttggc tgcgcgatac gttaatgcaa tgtgaaaaat cacaacgaaa acgcataatt
    12541 attgaacttg cagaggccga tgtaggtcaa catatcagtc gtttacaacc tgttattcgt
    12601 ttagtgaatg ctttaggggt acgggtagcc gtcaaccagg ctggtttgac gctggtaagc
    12661 accagttgga tcaaagaact taatgttgag ttactcaagc tccatccggg gctggtcaga
    12721 aacattgaga agcgaacgga gaaccagctg ctggttcaaa gcctggtgga agcctgctca
    12781 gggaccagca cccaggttta cgccaccggt gtgcgttcgc gaagcgagtg gcagaccctg
    12841 attcagcgcg gtgttacagg cgggcaaggg gattttttcg cgtcctcaca gccacttgat
    12901 actaacgtga aaaaatattc acaaagatac tcggtttaac ctgccgttta atccgttttc
    12961 acgtagaata acgcgcgctg cgtctcatgg gagtgtgctt gtctgctcgc cagattgttg
    13021 cagcacatat gcagatgaat gaccttacgc ggttgcaaac aggcgaggaa tgctgctgat
    13081 gcattaagcc tttctggact caggcagaga tttgtaacaa aggaaacgaa ctgcactaat
    13141 tttcaccgta gcagatgatt tttgcgcttt gtcgctgctg cgtgtggttg gtaaagtaag
    13201 cggattttct tttccgcccc agctttcagg attatccctt agtatgttga aaaaatttcg
    13261 tggcatgttt tccaatgact tgtccattga cctgggtact gcgaataccc tcatttatgt
    13321 aaaaggacaa ggcatcgtat tgaatgagcc ttccgtggtg gccattcgtc aggatcgtgc
    13381 cggttcaccg aaaagcgtag ctgcagtagg tcatgacgcg aagcagatgc tgggccgtac
    13441 gccgggcaat attgctgcca ttcgcccaat gaaagacggc gttatcgccg acttcttcgt
    13501 gactgaaaaa atgctccagc acttcatcaa acaagtgcac agcaacagct ttatgcgtcc
    13561 aagcccgcgc gttctggttt gtgtaccggt tggcgcgacc caggttgaac gccgcgcaat
    13621 tcgtgaatcc gcgcagggcg ctggtgcccg tgaagtcttc ctgattgaag aaccgatggc
    13681 tgccgcaatt ggtgctggcc tgccggtttc tgaagcgacc ggttctatgg tggttgatat
    13741 cggtggtggt accactgaag ttgctgttat ctccttgaac ggcgtggttt actcctcttc
    13801 cgtgcgcatt ggtggtgatc gtttcgacga agctatcatc aactatgtgc gtcgtaatta
    13861 cggttctctg atcggtgaag ccaccgcaga acgtatcaag cacgaaatcg gttcggctta
    13921 cccgggcgat gaagtccgtg aaatcgaagt tcgtggccgt aacctggcag aaggtgttcc
    13981 acgcggtttt accctgaact ccaacgaaat cctcgaagca ctgcaggaac cgctgaccgg
    14041 tattgtgagc gcggtaatgg ttgcactgga acagtgcccg ccggaactgg cttccgacat
    14101 ctccgagcgc ggcatggtac tcaccggtgg tggcgcactg ctgcgtaacc ttgaccgttt
    14161 gttaatggaa gaaaccggca ttccagtcgt tgttgctgaa gacccgctga cctgtgtggc
    14221 gcgcggtggc ggcaaagcgc tggaaatgat cgacatgcac ggcggcgacc tgttcagcga
    14281 agagtaatcg gatgcaggca ggggaagcgt ctgtttaccc tgcctggtct gatacgagaa
    14341 tacgcataac ttatgaagcc aatttttagc cgtggcccgt cgctacagat tcgccttatt
    14401 ctggcggtgc tggtggcgct cggcattatt attgccgaca gccgcctggg gacgttcagt
    14461 caaatccgta cttatatgga taccgccgtc agtcctttct actttgtttc caatgctcct
    14521 cgtgaattgc tggatggcgt atcgcagacg ctggcctcgc gtgaccaact agaacttgaa
    14581 aatcgggcgt tacgtcagga actgttgctg aaaaacagtg aactgctgat gcttggacaa
    14641 tacaaacagg agaacgcgcg tctgcgcgag ctgctgggtt ccccgctgcg tcaggatgag
    14701 cagaaaatgg tgactcaggt tatctccacg gttaacgatc cttatagcga tcaagttgtt
    14761 atcgataaag gtagcgttaa tggcgtttat gaaggccagc cggtcatcag cgacaaaggt
    14821 gttgttggtc aggtggtggc cgtcgctaaa ctgaccagtc gcgtgctgct aatttgtgat
    14881 gcgacccacg cgctgccaat ccaggtgctg cgcaacgata tccgcgtaat tgcagccggt
    14941 aacggttgta cggatgattt gcagcttgag catctgccag cgaatacgga tattcgtgtt
    15001 ggtgatgtgc tggtgacctc cggtctgggc ggtcgtttcc cggaaggcta tccggtcgcg
    15061 gttgtctctt ccgtaaaact cgatacccag cgcgcttata ctgtgattca ggcgcgtccg
    15121 acggcagggc tgcaacgttt gcgttatctg ctgctgctgt ggggggcaga tcgtaacggc
    15181 gctaacccga tgacgccgga agaggtgcat cgtgttgcta atgaacgtct gatgcagatg
    15241 atgccgcagg tattgccttc gccagacgcg atggggccaa agttacctga accggcaacg
    15301 gggatcgctc agccgactcc gcagcaaccc acgacaggaa atgcagctac tgcgcctgct
    15361 gcgccgacac agcccgctgc taatcgctct ccacaaaggg ctacgccgcc gcaaagtggt
    15421 gctcaaccgc ctgcgcgtgc gccgggaggg caatagtggc gagctatcgt agccagggac
    15481 gctgggtaat ctggctctct ttcctcattg cgcttttgct gcaaatcatg ccctggccgg
    15541 ataacctgat tgttttccgg ccaaactggg tgttactcat cttgttgtat tggatcctgg
    15601 ccttgcccca tcgcgtaaat gtgggcacag gttttgtgat gggtgccata ctggatctga
    15661 tcagcggctc gacgcttggc gtacgcgtat tggcgatgag catcattgct tacctggtgg
    15721 cgctgaaata ccagcttttc cgtaacctcg cattatggca gcaggcgctg gtcgtcatgt
    15781 tgctttcgct ggtggtggat attattgttt tctgggcaga gtttttagtg attaacgtct
    15841 ctttcagacc ggaagtgttc tggagtagtg tagtcaatgg ggtgctctgg ccgtggattt
    15901 tcttgctgat gcgtaaagtc cgtcagcagt ttgcagtgca ataaaggttt ctatgacttc
    15961 tctgtattta gcttccggtt ctccgcgtcg tcaggagtta cttgcgcaac ttggtgtgac
    16021 ctttgaacgt attgttacgg gcattgagga acagcgtcag ccgcaggaga gcgcgcagca
    16081 gtatgttgtg cgtctggcgc gcgagaaagc acgggcaggt gtcgcgcaaa cggcgaagga
    16141 tctcccggtg ctgggtgcgg atactatcgt tatcctgaac ggagaagtgc tggagaaacc
    16201 gcgcgacgca gagcatgcgg cgcagatgtt gcgcaaatta tcgggtcaga cccatcaggt
    16261 gatgacggca gtggcgttgg ccgacagcca gcacattctc gattgcctgg tggtcaccga
    16321 tgtgactttc agaacgttaa cagacgaaga catcgcgggc tatgtcgcca gcggtgaacc
    16381 gttagataaa gcaggtgcat acggtattca ggggctgggt ggctgttttg tcaggaagat
    16441 aaatggcagc tatcacgccg tagtcggctt accgctggtt gaaacgtatg aattattaag
    16501 taattttaac gcactgcgtg agaaaaggga taaacatgac ggctgaattg ttagtaaacg
    16561 taacgccttc ggaaacgcga gtggcgtata ttgatggcgg tattctgcag gaaattcata
    16621 ttgaacgtga ggcgcgacgc ggaatagtag gcaatatcta caagggtcgt gtaagtcgtg
    16681 tacttccggg tatgcaggcg gcttttgtag atattgggct ggataaagcc gcgtttcttc
    16741 atgcatctga catcatgccg cacaccgaat gtgtggcggg tgaagaacaa aagcaattca
    16801 cggtgcgcga catctcggaa ctggttcgtc aggggcaaga tctgatggtg caggtggtga
    16861 aagatccgct tggcactaag ggggcgcgcc tgaccaccga tatcacgctg ccttctcgct
    16921 atctggtctt tatgccaggg gcttctcacg ttggggtttc ccaacgaatt gaaagcgaat
    16981 cagaacgtga acgcctgaaa aaagtggtcg cagagtattg cgacgagcag ggcgggttta
    17041 tcatccgtac cgcagcggaa ggtgttggcg aggctgaact ggcctccgat gccgcttatc
    17101 tgaaacgcgt ctggaccaaa gttatggagc gtaaaaaacg cccgcagacc cgttatcagc
    17161 tgtacggcga actggcgctg gcgcagcgtg ttctgcgtga tttcgccgat gccgaactgg
    17221 accgcattcg cgttgactca cgcctgactt acgaagcatt acttgagttc acctcggagt
    17281 acattcccga gatgaccagc aagctggagc attacaccgg acgtcagccg attttcgatc
    17341 tctttgatgt cgaaaacgaa atccagcgag cgctggaacg caaagtagaa ctgaaatctg
    17401 gtggctatct gattatcgac cagaccgaag cgatgaccac cgtggacatc aataccgggg
    17461 cgtttgtcgg tcatcgcaat cttgacgaca ccattttcaa taccaatatt gaagcgacgc
    17521 aggctatcgc tcgccagtta cggttacgca atctgggcgg gattatcatt attgatttca
    17581 tcgatatgaa taatgaagat caccgccgcc gagtgctgca ctcgctggag caggcgttga
    17641 gcaaagaccg ggtgaaaacc agcgttaatg gtttttcggc gctggggctg gtggagatga
    17701 cgcgtaaacg cacccgcgaa agcattgagc acgtactgtg taacgaatgc ccaacctgcc
    17761 acggtcgcgg aacggtgaaa accgtggaaa cggtatgcta tgaaatcatg cgcgagattg
    17821 ttcgtgtcca ccatgcttac gactccgacc gtttcctggt ctatgcttct ccggcagtag
    17881 ctgaagcctt gaaaggcgaa gagtcacact cgctggcgga agtggaaatt ttcgttggta
    17941 aacaggttaa agtacaaatt gaaccgctct ataaccagga gcagtttgac gtcgtaatga
    18001 tgtaaacaga tgctgggccg ccatccggca aagggttttt gagtcacata tttagcagac
    18061 aaggagtgac gggtgaggcg attgccgggg attttactgc ttactggagc cgcgctcgtt
    18121 gtgatcgctg ccctgctggt tagcggcctg cgtattgctt taccgcatct tgacgcctgg
    18181 cgtccggaaa tcctcaacaa aatagaatcc gcgactggca tgccggtaga agccagtcag
    18241 ctctcagcca gctggcagaa ttttggcccg acgcttgaag cacacgacat ccgtgtagaa
    18301 ctaaaagatg gcggcgaatt ttcggttaaa cgcgttactc tggcgctgga tgtctggcag
    18361 agcctgttac atatgcgctg gcagtttcgc gatctcactt tctggcagct gcgctttcgc
    18421 accaacactc ctatcaccag cggtggtagc gatgacagtc tggaagccag tcacatcagc
    18481 gatctgtttc ttcgtcaatt tgaccatttc gatctccgcg acagtgaagt cagtttcctg
    18541 acgccatccg gtcagcgcgc cgagctggcg atcccacaac tcacctggct gaacgatcct
    18601 cgtcgacacc gtgcggaagg ccaggtaagc ctctccagcc ttaccggaca gcacggcgta
    18661 atgcaggtgc gcatggattt gcgcgatgat gaggggttgt taagcaatgg tcgcgtctgg
    18721 ctccaggcgg atgacatcga cctgaagccg tggctcggta aatggatgca ggacaatatt
    18781 gcgctggaaa cggcgcagtt ctcccttgaa ggctggatga cgatcgataa aggcgatgta
    18841 accggtggcg acgtctggct gaaacagggc ggtgccagct ggttgggcga gaaggaaacg
    18901 catacgctgt cggtggataa tctgaccgcg catattacgc gtgaaaatcc gggatggcag
    18961 ttctctgttc ccgatacacg gatcacgatg gacggcaaac cctggccgag cggagcattg
    19021 acgctggcct ggataccgga acaggacgtt ggcggcaaag acaataaacg cagtgacgaa
    19081 ctccggattc gcgccagtaa tctggagctg gcaggcctgg agggcatacg cccgctggcc
    19141 gcgaaacttt cacctgcact gggtgatgtt tggcgctcca cacaaccgag cggcaagatt
    19201 aacacgctgg cgctggatat cccgcttcag gcggcagaca agacccgttt tcaggcatcg
    19261 tggagcgatc tggcctggaa gcaatggaaa ttattaccgg gtgcggaaca cttctccggg
    19321 acgctttccg gcagcgttga aaatggtttg cttaccgcgt cgatgaagca ggcaaagatg
    19381 ccttacgaaa cggtattccg tgcgccactg gaaatcgccg acggccaggc aactataagc
    19441 tggctgaaca atgacaaagg tttccagctg gatgggcgta atattgacgt taaagccaaa
    19501 gccgtccatg cgcgcggcgg ttttcgttac ctgcaacctg ctaacgatga accctggctg
    19561 ggtattctgg ctggcatcag taccgatgat ggttcacaag cctggcgcta tttcccggaa
    19621 aacttgatgg gtaaagacct ggttgattac ttaagtggcg cgattcaggg cggtgaagcg
    19681 gataacgcga cgctggttta tggtggcaat ccgcaactct tcccctataa acacaacgaa
    19741 ggtcagtttg aagtgctggt gccgctgcgc aacgcgaagt ttgccttcca gccggactgg
    19801 cctgcattaa ctaaccttga tattgaactg gactttatta acgacggttt atggatgaaa
    19861 accgatggcg ttaatctggg cggcgtgcgc gcgagtaatc tcaccgcagt gatccctgac
    19921 tactcgaaag aaaaactgct gattgacgct gacattaaag gtccgggtaa agccgttggc
    19981 ccttactttg atgagacacc gctgaaagat tctctgggtg cgaccctgca agaactccag
    20041 ctcgacggcg atgtgaatgc tcgcttacat cttgatatcc cgctgaacgg cgaactggta
    20101 accgcgaaag gtgatgtgac gctgcgtaat aacagtctgt ttatcaaacc actcgacagc
    20161 accctgaaaa atttgagcgg taaattcagc tttatcaatg gcgatctgca aagtgaacca
    20221 ctgacagcaa gctggtttaa tcagccgttg aacgtggatt tttccaccaa agaaggggca
    20281 aaagcctacc aggtcgcggt gaatctcaac ggtaactggc aaccagcgaa aaccggcgtt
    20341 ctgcctgcag cggtgaacga agcattgagt ggcagcgtgg cgtgggatgg taaagtgggc
    20401 attgatctgc cttatcatgc tggcgcgacc tataacgtag agctgaacgg cgatctgaag
    20461 aatgtgagca gtcacttacc ttcaccgtta gccaaacctg cgggtgaacc actagcggta
    20521 aacgttaagg ttgatggcaa tctcaacagc tttgaattaa ccggacaggc tggtgcggat
    20581 aaccatttca atagccgctg gttgctcggt caaaagctga cgctcgatcg tgctatttgg
    20641 gcggcagaca gtaaaacgct cccgccgttg ccggaacaaa gtggtgttga actcaatatg
    20701 ccgccgatga atggtgccga gtggctggcc ctgtttcaga aaggtgcggc ggagagtgtc
    20761 ggtggtgcag cgagtttccc acaacacata acgttacgta cgcctatgtt gtcgctggga
    20821 aatcagcaat ggaataacct gagtattgtt tcgcaaccga cggcaaatgg cacccaggtt
    20881 gaggcgcaag ggcgtgaaat caacgccacg ctggcgatgc gtaataacgc gccgtggctg
    20941 gcgaatatca aatatcttta ttacaacccg agcgtggcga aaactcgtgg tgattcaaca
    21001 cagtcatcac ctttcccgac aacggaacgc attaacttcc gtggctggcc ggacgcccaa
    21061 atacgatgcg cagagtgctg gttctggggg caaaaattcg ggcgcattga cagtgatatc
    21121 accatttctg gcaatacatt aacgcttact aatggactga ttgatactgg tttttcgcgg
    21181 ctcactgccg atggtgaatg gattaacaat ccggggaatg aacgtacctc gctgaaagga
    21241 aaactgcgcg ggcagaaaat tgatgccgcc gcagaatttt ttggtgtcac gacgcccata
    21301 cggcagtcgt catttaatgt ggattacgat ttacactggc gtaaagcacc gtggcagcca
    21361 gatgaggcga cgttgaatgg catcattcat actcaactgg gtaaaggcga aattaccgaa
    21421 atcaataccg gacatgccgg gcaattgctg cgcttattga gcgtagatgc cctgatgcgt
    21481 aagctgcgtt ttgatttcag agacactttt ggcgaagggt tctattttga ctccattcgc
    21541 agcaccgcgt ggattaaaga cggcgttatg cacaccgacg acacgctggt ggatggcctg
    21601 gaggcggata tcgccatgaa agggtcggta aatctggtac gtcgcgacct gaatatggaa
    21661 gcggttgtcg caccagagat ttctgcgacg gtgggcgtgg ctgcggcttt tgcggttaac
    21721 cccattgttg gcgcggcagt gtttgccgcc agtaaagtgc tggggccgct gtggagcaaa
    21781 gtctccattt tgcgctatca catttcgggt ccgctggacg atccgcaaat caacgaagtg
    21841 ttgcgccaac cgcgtaaaga aaaagcgcaa tgatttgacg agggcgcgta attgccccaa
    21901 tctcataaga taatcgttgc caaaggccaa cgagccagaa cataaccgta ggccggatag
    21961 ggcgtttacg ccgcatccgg cagccgtaaa aaatcctcta ctgcagtaac taacgagtag
    22021 caaaaacgat gagtctgaac ctggtaagtg aacaattgct agcggcgaac ggcctgaaac
    22081 atcaggactt gttcgcgatc ctcggtcaac tggccgaacg tcgccttgat tatggcgatc
    22141 tctattttca gtcgagctat cacgaatcct gggttttaga agaccgtatt attaaagatg
    22201 gttcttacaa catcgatcag ggcgttggcg tgcgtgcaat cagcggtgaa aaaaccggat
    22261 ttgcttacgc tgaccaaatc agcctgctgg cgctggaaca gagtgcgcaa gcggcgcgca
    22321 ccatcgtccg tgatagtggt gatggcaaag tacagacgct gggcgcggta gagcatagcc
    22381 cgttgtatac ctcggtagat ccgctgcaaa gcatgagccg tgaagagaag ctggatatcc
    22441 tacgtcgcgt cgataaggtt gcccgcgaag cggacaagcg cgtacaggaa gtgactgcca
    22501 gcctcagtgg tgtctatgaa ttaattctgg ttgcggccac cgacggcacg ctagcggcgg
    22561 atgtccgtcc gctggtgcgt ctttccgtga gcgttctggt cgaagaagat ggcaaacgcg
    22621 aacgcggtgc cagtggcggc ggcggtcgtt ttggttatga gttcttcctt gccgatcttg
    22681 acggcgaagt ccgtgcggat gcatgggcaa aagaagcagt gcgtatggcg ctggtcaatc
    22741 tttctgccgt tgctgcacca gcgggcacca tgccggtagt acttggcgca ggttggccgg
    22801 gcgtgctgtt gcatgaagcg gtaggtcacg gtctggaagg cgacttcaac cgccgtggca
    22861 cttcagtatt tagtggacag gtcggggagc tggtggcttc agaactgtgt accgtggttg
    22921 atgatggcac gatggtcgat cgccgaggtt cggtggcgat tgatgacgaa ggtacgccag
    22981 gccagtacaa cgtgctgatt gagaacggca ttctgaaagg ctacatgcag gataaactca
    23041 acgcgcgttt gatggggatg acgccgactg gcaacggtcg ccgtgaatcc tacgcccatc
    23101 tgcccatgcc gcgtatgacc aacacctata tgctgccggg taaatcgacc ccgcaggaaa
    23161 ttattgaatc cgttgagtac ggtatctatg caccgaactt tggtggcggt caggtggata
    23221 tcacctccgg caaattcgtt ttctccactt cagaagcata tctgattgaa aacggtaaag
    23281 taacgaagcc ggtgaaaggc gcaacgttga ttggttccgg tatcgaaacc atgcagcaga
    23341 tttcgatggt tggcaacgac ctgaaactgg ataacggcgt gggtgtctgc ggtaaagaag
    23401 ggcaaagttt gccggttggt gtgggccagc cgacgttgaa agtcgataac ctgactgttg
    23461 gcggtactgc gtaataattt cattattttc agaaggataa ttaatctttc tacgtgcacg
    23521 aacggtcccc tcgctcctct ggggttaggg tgaggggaac ccgttggcac aggtttgtac
    23581 aacgtaacag tacaatatga attacttctc tttcccgcgc ccgtgcatct cctgaaacaa
    23641 tttaccgacc tcaacaaaat aatccgtcag cgagttgatc acgacctgta ccttcagcgg
    23701 cagcttatct ttttcggtat ataacgcata aaccgggcgt ggatctgact gataacgcgg
    23761 cagcaggatc tccagctccc cacgattgat ctcgttgatc acccacatca gcggcacgta
    23821 ggcgatcccg gcccccgccg ttagccagcg caccagcgtc atcggatcat tagtcacaaa
    23881 tctcccttgc gggatcaggc gagtcgagat cccttccggt gcaatcagtt caaattcatt
    23941 gtcgggccgc acgctgtatt caagccatga atggctactt aaatcggcgg gtttttccgg
    24001 tatgccgtat tgtgtgagat agcttttcgc ggcgcacacc accattggca tcgcgccaag
    24061 acggcgggaa aacaggctgg aatcctgcaa cgcgccgacg cggatcacca catccagacc
    24121 gtcggcaatc aggtcggggg ctggaattcc ggtaaccaga ttgacgctca aacctgggta
    24181 ttctttcagc attttggctg tcagcccggc gagaacattt tgtgccatag ttgaagaaca
    24241 gccaatgcgt agcgtcccga tgggggtgtt attgaaggca tacagttgct catgaacatc
    24301 ctgcacttca tgaagcatac gacggcagcc ctggtagtaa attctaccgg cttcggtcag
    24361 gccaatgctg cgtgtgctac ggtttaacag ctttacctgc aactcatctt ccagttttga
    24421 caccgtctga ctgatggacg aaacgctcat ctgtagctgt ctggcggcgg cggtaaaaga
    24481 gccaaattca actactttgg caaacaccga catgcgtttt agtcgttcca ttattcactc
    24541 tgacttaaaa gtgatttaga tcacataata tagataacag cataacagtt acgctaatat
    24601 attaaatatc aatctacagc aatgttgctc tcgcccggct ttccatgccc ttctctgcgg
    24661 cgacagatgc tgaaaataat aacgcctgct ctctctatac aaccaaggtc aacatgagtc
    24721 tgtttcccgt tatcgtggtg tttgggctgt ccttcccacc gatatttttt gaattgcttt
    24781 tatcactggc gattttctgg ctggtgcgcc gggtacttgt gccaacaggt atctacgact
    24841 ttgtctggca tccggcgttg ttcaacaccg cgctctattg ctgcttgttt tatttgatat
    24901 cgcgactgtt cgtttgaggt tgaagtgaaa acactaataa gaaaattctc ccgtacggcc
    24961 atcacggtcg tattagtcat tctggccttc atcgcaattt ttaatgcctg ggtctattac
    25021 accgaatccc cctggacgcg tgacgcgcgc tttagcgctg acgtcgttgc gatcgcgccg
    25081 gacgtttctg gactcattac ccaggtgaat gttaatgata accagctggt gaaaaaagga
    25141 caggttctgt tcaccatcga ccagccgcgc tatcaaaagg cgcttgagga agcgcaagcc
    25201 gatgttgctt attatcaggt actggcacag gagaaacgcc aggaggccgg acgtcgtaac
    25261 cgtctcggtg tgcaggcgat gtctcgcgaa gagatcgacc aggccaacaa cgtactacaa
    25321 acggttctgc atcagttagc gaaagcgcag gcgacccgcg atctggcaaa actggatctt
    25381 gaacgcacgg tgatccgcgc gccagcagat ggctgggtga ccaacctcaa cgtctatacc
    25441 ggtgagttta ttactcgtgg atcaacggcg gttgcgctgg tgaaacagaa ctctttctat
    25501 gtactggcct atatggaaga aactaagctg gaaggggtgc gccctgggta tcgtgcagag
    25561 atcacgccgc ttggcagtaa caaagtgctg aaagggactg ttgatagtgt tgccgctggg
    25621 gtcaccaacg ccagcagcac gcgtgacgac aaagggatgg cgaccatcga ctctaacctt
    25681 gaatgggtgc gtttggcgca acgtgttccg gttcgtattc gtctcgacaa ccagcaagag
    25741 aacatctggc ctgcgggcac cactgctaca gtggtggtca ctggcaaaca agatcgcgac
    25801 gaaagccagg attcgttctt ccgtaaaatg gcccatcgcc tgcgtgagtt tggttaatca
    25861 cgatgggtat tttctccatt gctaaccaac atattcgctt tgcggtaaaa ctggcgaccg
    25921 ccattgtact ggcgctgttt gttggctttc acttccagct ggaaacgcca cgctgggcgg
    25981 tactgacagc ggcgattgtt gccgccggtc cggcctttgc tgcgggaggt gaaccgtatt
    26041 ctggcgctat tcgctatcgt ggctttttgc gcatcatcgg cacatttatt ggctgtattg
    26101 ccggactggt gatcatcatt gcgatgatcc gcgcaccatt attgatgatt ctggtgtgct
    26161 gtatctgggc cggtttttgt acctggatat cctcgctggt acgaatagaa aactcgtatg
    26221 cgtgggggct ggccggttat accgcgctga tcattgtgat caccattcag ccggaaccat
    26281 tgcttacgcc gcagtttgcc gtcgaacgtt gtagcgagat cgttatcggt attgtgtgtg
    26341 cgattatggc ggatttgctc ttttctccgc gatcgatcaa acaagaagtg gatcgagagc
    26401 tggaaagttt gctggtcgcg caatatcaat taatgcaact ctgtatcaag catggcgatg
    26461 gtgaggttgt cgataaagcc tggggcgacc tggtgcgacg caccacggcg ctacaaggca
    26521 tgcgcagcaa cctgaatatg gaatcttccc gctgggcgcg ggccaatcga cgtttaaaag
    26581 cgatcaatac gctatcgctg acgctgatta cccaatcctg cgaaacttat cttattcaga
    26641 atacgcgccc ggaattgatc actgatactt tccgcgaatt ttttgacacg ccggtagaaa
    26701 ccgcgcagga cgtccacaag cagctcaaac gcctgcgaag agttatcgcc tggaccgggg
    26761 aacgggaaac gcctgtcacc atttatagct gggtcgcggc ggcaacgcgt tatcagcttc
    26821 tcaagcgcgg cgttatcagt aacacaaaaa tcaacgccac cgaagaagag atcctgcaag
    26881 gcgaaccgga agtaaaagta gagtcagccg aacgtcatca tgcaatggtt aacttctggc
    26941 gaaccacact ttcctgcatt ctgggaacgc ttttctggct gtggacgggc tggacttccg
    27001 gcagtggtgc aatggtgatg attgcggtag tgacgtcact ggcaatgcgt ttgccgaatc
    27061 cacgcatggt ggcgatcgac tttatctacg ggacgctggc cgcgctgccg ttagggctgc
    27121 tctacttttt ggtgattatc cctaataccc aacagagcat gttgctgctg tgtattagcc
    27181 tggcagtgct gggattcttc ctcggaatag aagtacagaa acggcgactg ggctcgatgg
    27241 gggcgctggc cagcaccata aatattatcg tgctggataa cccgatgact ttccatttca
    27301 gtcagtttct cgacagcgca ttagggcaaa tcgtcggctg tgtgctcgcg ttcaccgtta
    27361 ttttgctggt gcgggataaa tcgcgcgaca ggactggacg tgtactgctt aatcagtttg
    27421 tttctgccgc tgtttccgcg atgactacca atgtggcacg tcgtaaagag aaccacctcc
    27481 cggcacttta tcagcagctg tttttgctga tgaataagtt cccaggggat ttgccgaaat
    27541 ttcgcctggc gctgacgatg attatcgcgc accagcgcct gcgtgatgcg ccgatcccgg
    27601 ttaacgagga tttatcggcg tttcaccgac aaatgcgccg cacagcagac catgtgatat
    27661 ctgcccgtag cgatgataaa cgtcgccggt actttggcca gttgctggaa gaacttgaaa
    27721 tctaccagga aaagctacgc atctggcaag cgccaccgca ggtgacggaa ccggtacatc
    27781 ggctggcggg gatgctccat aagtatcaac atgcgttgac tgatagttaa gtcaaaaccg
    27841 acgccaaaag cgtcggtttt ttcatggcta tacttagcga tgcacggcag aactcgccgc
    27901 gaaacgtgac ggtggcaaca gatgaatatt tatacctttg attttgatga gattgagagt
    27961 caggaggatt tttatcgtga ctttagccaa atctttggtc tggcgaaaga taaggtacgc
    28021 gatctcgact cactatggga tgtgttaatg aacgatgtcc tgccgctacc acttgagatt
    28081 gaatttgttc atctgggaga gaaaacgcgt cgccgttttg gcgcgttaat attgctgttt
    28141 gatgaggcag aggaagagct ggaagggcat ttgcgtttta atgttcgtca ttagcgcaaa
    28201 aaaaagcccc cgaaccgggg gcaatatcgt cggacaagac gatgagggtt tatttgtaca
    28261 gttcagccgt agcgtgccag gtgtcaccgc tacgagcttc agtaatctgg taggccgttg
    28321 cgcctttctc ttccgctttt ttgttcagca tttcacgcat atccattggc gaagacgcca
    28381 caccacttac ggatacggtc ccgattgctt cacgattttg tgcttgtgca gcatcaatgg
    28441 agtcggcagc gaatgcaccg aaagagagaa cagaaagtac gcttaatgca gcaacagtgg
    28501 ttttgatttt catgatattt acctcaacat aatctttttg ctgggtcttt gtttcgtgac
    28561 ccttatcaca aaatcaagta tacactaatt attggactaa ttaataccac gctaactgtt
    28621 tctgttgata ctaagt
//
LOCUS       DAZUCE010000039        27938 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZUCE010000039 DAZUCE010000000
VERSION     DAZUCE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.039"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            18..1373
                     /locus_tag="ACHY03_001484"
     CDS             18..1373
                     /locus_tag="ACHY03_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HFS3904731.1"
                     /translation="MARFQFKSRKNNGFIFLISFMVMGEATIAAPLPQWANAPAVTPV
                     AQLSLQESILRAFARNPDVTQQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDS
                     SGRLDNNVSYGITLTQLVYDFGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYM
                     EVSRYQALCDAAQRNIHSLENVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQ
                     AQMASAKAQLAVLTGVQPEAIAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQY
                     GVEKTKAQYWPTLSIQGGKTRYQTSDRSYWDDQLQLNVNAPLYQGGAVSAQVQQAEGQ
                     QKISASQVEQAKLDVLQRASVAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGK
                     RSLNDLLTVEQDVFQAQSAEINANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDL
                     K"
     gene            1476..22253
                     /locus_tag="ACHY03_001485"
     CDS             1476..22253
                     /locus_tag="ACHY03_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS3904732.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHVTFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTTVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDTTGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQSDGTWSVTVPAANMGAL
                     ADGNATVTASVNDVAGNPSSVSRVALVDATPPVVTINPVATDNVINTPEHTQAQIISG
                     TVTGAQAGDIVTVTLNNVDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADLQITGTSDQPVGTAITVTLNG
                     QNYAATTDASGNWSVTVPASAVTALGQANYTVTAAVTSSIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAAVGDTVTVTLGGNTYTTTVQANLSWSVS
                     VLAADIQALGNGDLTVSASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAEGTPLTVTINNVEYTTAVQADGSWSVGVTAAQVSTWPAGTVNI
                     AVSGESSAGNSVSITHPVTVDLTPAAITINTIATDDVINAAEKGADLTLSGTTTNVEP
                     GQTVTVTFGGKNYTASVASDGSWTATVPAADLASLPEGSASAQASVSNINGNSASAVH
                     NYSVDSSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTVTLNSPTVQTYQ
                     ATVQADGSWSINIPAADLEALTDGSHTLTATVNDKAGNPASTTHNLAVDLTVPVLTIN
                     TIAGDDIINATEHGQALVISGSSTGGEAGDVVTVTLNSKTYTTTLDASGNWSVGVPAA
                     DVTALGSGPQTVTATVTDAAGNSDSETHTVTVNLTAPTIGINTIATDDVINATEKGAD
                     LQISGTSNQPAGTTITLTLNGQNYTATTDASGNWSTTVPASAVGALGEASYTVTANVT
                     DSAGNSNSASHNVQVNTALPGVTINPVASDDIINAAESGVAQTISGQVTGAAAGDTVT
                     VTLGGKTYTATVQGNLNWSVDVPAADIQAIGNGDLTVNASVTNGVGNTGSGSREIVID
                     ANLPGLRVDTVAGDDVVNSIEHGQALVITGSSSGLAAGAALTVVINNVTYGATVLADG
                     TWSLGVPAANVGNWPAGTVNITVSGATSAGNPVTITHPVTVDLAAVAISINTVSGDDV
                     INAAEKSADLTLSGSTSGVEVGQTVTVTFGGKTYTATVAGDGSWTTSVPAADLAALRD
                     GDATVQASVSTINGNTASATHAYSVDATAPTLAINTIATDDILNAAEAGNPLTISGSS
                     TAEAGQTVTVTLNGVTYTGTVQADGSWSVSVPTADLSNLTASQYTVSASVSDKAGNPA
                     SANHGLAVDLTVPVLTINTVSGDDIINATEHGQALVISGSSTGGEAGDVITVTLNSKT
                     YTTTLDASGNWSVGVPLSDVTALGSGPQTITATITDIAGNSDDASRTVTVNLTAPTIG
                     INTIATDDVINATEKGADLQITGTSNQPVGTTITVTLNGQNYTATTDSSGNWSATVPA
                     SAVSTLGETNYTVTANVTDSAGNSNSASHNVLVNSALPAVTINAVATDDIINAAEAGN
                     AQTISGQVTGAAAGDTVTVTLGGNTYTATVQANLSWSVSVPAADIQALGNGDLTINAS
                     VTNVVGNTGSGSRDITIDANLPGLRVDTVAGDDVINSIEHNQALVITGSSTGLTAGTA
                     LTVEINNVTYGATVLADGTWNLGVPAADVSNWPAGTVDITVSGTNSAGTTSTITHPVT
                     VDLSAVAITINTLSGDDVINAVEKGETLVVSGSTSGVEAGQTVTVIFGGKNYTTTVEA
                     NGSWTVNVPPADLAALPDGAGNVQASVSNINGNNAQADRTYSVDATAPLVTINTIASD
                     DILNVSEAGAGITISGTTTAQAGQTLTVTLNNNTYQTTVQADGTWSVNVPAADLSGLT
                     ASSYTVTASVSDKAGNPESADHALAVDVTAPDLTINTVSGDDIINAIEHGQALVVSGI
                     STGAEPGDVVTVTLNGKNYTTTLDASGNWSVGIPAADVTALATGSQTITASLSDRAGN
                     SDSTTHNVTVDLSGPTLTISIVSGDDIINNAEKTQDLIISGGSSGLATGTTVTVMLNG
                     LAYSATTDGSGNWSVTVPASAVGALGEAVYQISASATDSAGNSGITTHTVNVESLLPG
                     VIINTVAGDDIINAAEIAVAQTISGQVTGTAAAGNTVTVIIGGNQYTATVQPDLSWSV
                     SVPANVLQALGNGELTISASVTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSI
                     EHTQALVITGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPAADVTNWPAGTVN
                     IAVSGTNTAGTTTSITHPVTVDLAAVAITINTLSTDDVINAAEKGSDLQLSGTTSGVE
                     AGQTITVIFGGKSYTTTVATDNSWELTIPAADLATLPDGAANVQASVSNVAGNSAQAT
                     HAYSVDATAPSVTINTIASDDILNAAEAGSALIISGTSTAEAGQTVTVTLNGVNYSGN
                     VQADGSWSVSVPTGDLASLTASSYTVNASVSDKAGNPASATHNLTVDLVAPVVTINTV
                     AGDDIINATEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVI
                     SALAQGDVTITATVTDSAGNSGTASHTVSVALGAPVLGINTIAVDDIINATEKGADLA
                     ITGSSNQPAGTQITVTLNGQNYTTTADASGNWSVTVPASAVGTLGEATYTVTASATDA
                     DGNSGSASHNVQVNTALPGVTINVVATDDIINAAEAGVDQTISGQVTGAAAGDTVTVT
                     LGGATYTATVQANLSWSVDVPASALQALGNGELTVNASVTNAVGNTGSGTRDITIDAS
                     LPGLRVDTVAGDDVVNIIEHGQALVITGSSSDLAAGSNVTLTINGQTYVAAVLADGSW
                     SVGVPAADVSAWPAGTVTITASGNTTAGNPVSVTHPVTVDLTAVAVSINTITVDDVIN
                     AAEKGAALTLSGSTSGVEAGQTVTVTFGGKTYSATVAANGSWSTTVPAADMAALRDGD
                     ASAQASVSNVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTA
                     EAGQTVTVTLNGTNYTGTVQTDGSWSVSVPPADLSALTASNYTVSAAVNDKAGNPASV
                     NHNLTVDTSVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFT
                     TVLDASGNWSVGVPASVVSALANGTVTINASVTDAGGNSGSTTHQVMVNTGLPTITFN
                     AISGDNVLNADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVS
                     DLAALGQANYTVSASATSAAGNTASSQANLLVDSGLPGVTINTVAGDDIINAAEAGAD
                     QTISGVVTRAAAGDTVTVTLGGNTYTAEVQPDLSWSVTVPTDDLQALGNGDLTITASV
                     TNANGNTGSGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGAVL
                     TVTINSVAYSATVQADGSWSVGIPAANVSAWPAGPLAVDVAGQSSAGNPVSVSHPFTV
                     DLTAVAISINTVASDDVINAAEKGTDLTLSGSTSGIESGQTVTVTFGGKTYTASVAAN
                     GSWSVNVPAADLATLPDGAANVQASVSSASGNSASATHAYNVDASAPTLTINTIASDD
                     ILNATEAGSPLTISGTSTAETGQTVTVTLNGATYSGNVQADGSWSVSVPTSALGALTA
                     SNYTVSATVNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTS
                     TGGEAGDVVSVVLNGKTYTTTLDASGNWSVGVPAADVTALGSGAQTITASVSDRAGNS
                     DDASRTVTVSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAITVTLNGQN
                     YSATTDASGNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGIT
                     INPVATDDIINASEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVP
                     AADWQALGNGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHA
                     QAQVITGSSSGFAAGTALTVVINNQTYAATVLANGTWSVGVPATDVSNWPAGTLNITV
                     SGANSAGTQTSITHPLTVDLTTVAVSMNSITSDDVINVAEKGAALTLSGSTSGVEAGQ
                     TVTVTFGGKNYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEY
                     SVDSAAPTVTINTIASDNIINASEAAAGVTVSGTSTAQTGQTLTVTLNGTNYQTTVQA
                     DGSWSLTLPASDLTALANNGYTLTATVSDLAGNPGSASKGVTVDTTAPVISFNTVAGD
                     DVINNVEHIQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGL
                     ADGTVTISATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNIINAAEAGSALTLSG
                     TGTNFATGTVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALSDGTSYTVSASAQDSA
                     GNSATASRSVAVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFG
                     GKTYTATVAANGTWALNVPAADLAALGQGAQTITASVNDRAGNPGQATHALTVDTVAP
                     TVTIATVAGDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQTWSATVGSGGSWSVF
                     IPAQQFAGLSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAE
                     HGSSLVISGTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVI
                     NASVSNAIGNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSL
                     TAALASNETAQISIDGGVTWITLTVTGTTWHYNDSRTLTDGNYLYQVRVIDAAGNVGA
                     TDSQNVVIDTTAPDPAVKTIAISAITTDTGLITNDFVTSDTTLAVSGTLGAALSAGEF
                     AQISIDGGTTWQNLSVSGLTWTWLDGRTLTDGNYNYQVRVIDTAGNIGATASQIVTVD
                     TTAPLASKTIAIAGISDDTGLSSSDFVTRDTTLTVRGTLGAALAADERAQISLDGGVT
                     WTTLTVVGTSWSYADGRTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSI
                     TITGISDDTGASSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTLAADG
                     LNWSYVDGRTLTNGTTTWQVRVVDLAGNVGATGSQSAQIDTVNPAQVLTIASISTDTG
                     SSATDFITSDTTLTLTGSLGAGLASGEVAQISLDSGATWITLTTNGTQWTYTDSRTLT
                     DGSYVYQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQAT
                     DDTTPLLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSAR
                     VVDLAGNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVV
                     INGKTYTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNANI
                     SNGTVTVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKT
                     ALTLYQSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTV
                     GTLTWYGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATV
                     GGAVTGYLSLNEGSGVDLNNDGRIDLVQHTYNLNNYYTLSSLINQGNGTFVWGQNTTN
                     TFLSGAGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPVAV
                     GATATTYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVGGASNWTQSALGGSQSGT
                     TSGVAAMDYDWDGAVDVLVTKQSGSVFLIRNTNTVSYGTSLHLRITDPNGINVYYGNT
                     VKLYNSAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNID
                     QTVNTSWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGW
                     VYSSGTGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSNFN
                     DTLTGSSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTW
                     EGTADTDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQ
                     IDRDGTGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            22257..24419
                     /locus_tag="ACHY03_001486"
     CDS             22257..24419
                     /locus_tag="ACHY03_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HFS3904733.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERDSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADVEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWNLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAVFYYDEEEKIADVAIGKLNIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALILSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAYLIEQLKQWIGHRTLVIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQSEPTARRVVLQEKNKGSAA"
     gene            24416..25591
                     /locus_tag="ACHY03_001487"
     CDS             24416..25591
                     /locus_tag="ACHY03_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFS3904734.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLDVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            25588..25995
                     /gene="cueR"
                     /locus_tag="ACHY03_001488"
     CDS             25588..25995
                     /gene="cueR"
                     /locus_tag="ACHY03_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFS3904735.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDESADCPIIENLSGCCHHRAG"
     gene            complement(25996..27219)
                     /locus_tag="ACHY03_001489"
     CDS             complement(25996..27219)
                     /locus_tag="ACHY03_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904736.1"
                     /translation="MLFPCRKPEVRLLKNTAAGVIFSVKSGKGFLRQCVTYDPHPYGL
                     IRSLCWQDVPLIRFWSEADCPTCAEFVYSGFADNEQGAARFLSALENWNRPWSGMTDA
                     FATLTPLFSCLADGYYLLEDRELYPTDGNGHFFWAATDHSSSNPATVAVWDQEYCSFS
                     DTAPCFLLPGQPPSHFNPERATFYRDKPDARALAWYLTDSYLCVLLDGHHKATAAALE
                     GRPLKTLVLSTATRFNDEQQTLVFPGGEFLHKTELQYRIPKLTAWKKLPPSAWDSFGP
                     DKHVSPLETWSEELQQSVSRYPSLDQVWQIVEAGNLSETRIKRIIQQGLGEDEKADVI
                     LQALFFTHSPLFVDFARVVISHPVYAIYRPLTFRLMAQTRTPQADAFFLDFAINDDGE
                     RPELTKIMDDYFRKP"
     gene            complement(27308..27514)
                     /locus_tag="ACHY03_001490"
     CDS             complement(27308..27514)
                     /locus_tag="ACHY03_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904737.1"
                     /translation="MKEQRKIPLTHIMIIGAFIFAFLQVVLLASLVHAVNVNNEIQEG
                     LFQSGRIMVESLQHILSVQTGING"
     gene            complement(27511..27933)
                     /locus_tag="ACHY03_001491"
     CDS             complement(27511..27933)
                     /locus_tag="ACHY03_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904738.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGMTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKIALMKEGQFVGYLK"
BASE COUNT         6439 a         8198 c         8248 g         5053 t
ORIGIN      
        1 aaggaagtga cttatctatg gcgcgattcc agtttaaaag ccgtaaaaat aatgggttta
       61 tttttttaat aagttttatg gtgatgggag aagctacaat tgctgcaccg ctgccacaat
      121 gggcgaacgc cccagcggta acgccagttg cccaattatc cttacaggaa agtatattac
      181 gcgcctttgc gcgaaatccc gacgtcactc aacaggcggc gcagataggc attggcgaag
      241 cgcaaattga tgaagccaaa agtgcctggt atccgcacgt tggattaacc ggcaacgcgg
      301 ggccgtcccg acaaacggac tccagcggca ggcttgataa caacgtttcg tatggcataa
      361 ccctgacaca gctggtgtat gactttggta aaactaacaa cgatatcaat ctgcaaactg
      421 ccgcccgtga cagctaccgc tttaaattga tggcaacctt aaccgatgtt gcagagaaaa
      481 cggcgactgc ctacatggaa gtcagtcgtt atcaggcttt gtgcgatgcg gctcaacgca
      541 atattcactc gctggaaaac gtctacaaca tggcggcatt gcgcgctaac gcaggcctga
      601 actcatcgtc ggatgaatta caggcccaga cgcgtattgc cggaatgcgc tcaacgctgg
      661 agcaatatca ggcgcagatg gcgagcgcca aagcgcaact ggcggtgctc actggcgtac
      721 agccggaggc gatagccgcg ccacctgctg aattagccga gcagccggta tcgctgaaga
      781 atattgatta ccagtccatc ccgctggtgc tggcggcaga aaacttacgc cagtcagcac
      841 agtacggcgt ggaaaaaacg aaagcgcaat actggccaac gctcagtatt caggggggta
      901 aaacgcgcta ccagaccagc gaccgctcgt attgggatga tcagctacaa ctgaacgtta
      961 acgcgccgct gtatcagggc ggcgcggttt ctgcccaggt gcaacaggcc gaagggcaac
     1021 aaaaaatctc tgcctcgcag gtcgaacagg caaaactgga tgtgctgcaa cgagcgtctg
     1081 tggcatatgc gaactggacc ggcgcacggg gccgtgaaga agccggttta gcgcaatccg
     1141 aaagtgcgca caaaacgcga gatgtgtacc aaaatgaata taaattgggt aaacgcagtc
     1201 tgaatgatct attaaccgtc gagcaagatg tctttcaggc gcaatcggct gaaataaatg
     1261 ccaattatga tggctgggtc gccgccgtaa attatgccgc tgcggtgaat aaccttattc
     1321 cattagcggg aattaaacag ggcttataca acgacttacc cgacttgaaa taatcctgcc
     1381 attattaata gtgggccgtt gttaagtgat tacctttgtc ttatttagat aaatggactc
     1441 gctaaggatg tgcgccattt atcaggaatg ccattatgag cctgattatt gatgttattt
     1501 cgcgtaaaac atccgtcaaa caaacgctga ttaatcctgg cgacgtcacg gttgttattt
     1561 atgagccttc cgtggtgcag gttcatgctc aggcctctgc cgttgcgcgt tacgtccgtg
     1621 aaggaaatga cctgctgatc tatatgcagg acggcacggt gatccgctgc aacggttatt
     1681 tcctgcaagc ggcgaataca gctgaacaat cggaactggt gtttgccgat gggcaacagc
     1741 taacccatgt caccttcgcc gatactgctg cgggtggatt agcccccgta gaactgactg
     1801 cccagaccac tgcgattgaa agcattgcgc cgtttcttga taccgttgct cagaccagcg
     1861 ccttcccgtg gggttggctg gcgggggcgg cggtaggtgg tggcgcgctt ggtgcactgc
     1921 tggcaagcgg tggcgatggc gactcgaaaa cagaagtgat taataaccct acgccacctg
     1981 ctgagcctgg caacgccaca ccatcatttt tagttaccga taatcagggc gatcagcgcg
     2041 gcattctcgc caccaatgac atcaccgatg acaccacgcc aacctttagc ggcagcgggc
     2101 aggcgggggc gactattcag attaaagaca gtaacggcaa tactattgcc agtactcagg
     2161 tagacaacaa cggtcactgg agtgtctcgt tacccacgca aagtgcaggt gaacatacct
     2221 ggtcagtggt gcaaattgtc ggcagtacca tcactgacgc cggttcgata acgttaacca
     2281 tcgacaatag tcaggccagc gtgcaggttg ccaccaccgc aggcgataac attattaacg
     2341 ccagcgaaca ggccgccggg tttacgcttt ctggcaccag tagccatctg gcgcagggaa
     2401 cagaactcac cgtcacactt aacggcaaaa cctacaccac aactgtaggg gctaacggtg
     2461 cctggagcgt gcaggtgccg accgccgatg cacaggcgtt aggcgaaggg aatcaggcgg
     2521 tactggtcag tgggaaagac accacaggca atacggtcac cggcgcgcag ctgctaacgg
     2581 tcgataccca accgccaacg cttgccatca acaccatcgc ccaggacaac atcatcagtg
     2641 ctgcggaaca taacgtcgcg ctggtactga gcggcacgtc gaatgcagaa gcggggcaaa
     2701 ccgtaacact gaccgtcaac gggaaaagcc atacagcaac cgtcggtagc gacggaacct
     2761 ggcaagtgac gctgcctgcc acggaggtcc aggcactggc agagggtaat tacgctgtca
     2821 atgccagtgt cagcgatcgg gcagggaaca ccaccagcca cagcgccaat ttcacggtag
     2881 acacctcagc acccgtggtc agtgttaata ccgtggcggg cgacgatatt cttaataatg
     2941 ccgagcaggc cgtcgcgcag atcatctccg gacaagttag cggtgcttct cctggcgata
     3001 cggtaacggt gaaattgggc actcatgtcc tgacgggcat cgtgctggca gatggcagct
     3061 ggaatgtggc gctggacccg gcggtaaccc gcacgctgga tcgcggagcc aatacgattt
     3121 tcgtcaccgt gacagatgct gcaggaaata ctggcgcggc gtctcgagca atcacgctgg
     3181 tcggtgtttc tccgttgatc accattaaca ccgtctccgg cgatgacatt atcagtggcg
     3241 cagaaaaagg tgcgccactg acccttaccg gtagcactca acaggctgag acaggacaaa
     3301 ccgtcacagt aaccctggct ggacagagtt ttaccactac cgtgcaggcc gatggctcct
     3361 ggagtctgac ggtacctgcc gccgcgatgg gaaatctgcc tgacggcgcg gtggcgatta
     3421 ccgcttctgt gacggatctc agcggcaata ccggcaacac ctcccgcacc attaccgtcg
     3481 acagccaggc cccggcctta agcattgatc cactgaccgc tgataacatc attaacgccg
     3541 ccgaaagcgg gcaggatctg cccatcaccg gcaccaccga cgcccagcca gggcagacgg
     3601 tgacggtgac gctgaacggg cagacgtatc agggcgtcgt gcagtcagac ggcacctgga
     3661 gcgtgactgt gcccgccgcc aacatgggcg cactggctga cggcaacgct acggtcaccg
     3721 ccagcgtgaa cgatgtcgcc ggtaatccga gcagcgtttc gcgcgtggct ctggtggatg
     3781 ccacgccgcc ggtggtaaca attaatccgg tggcgaccga taacgtcatc aacacgccgg
     3841 aacataccca ggcgcaaatc atcagcggta cggttactgg cgctcaggcg ggcgatatcg
     3901 tcaccgtgac gctgaataat gtggattaca ccacggtggt ggatggttcc ggcaactgga
     3961 gtctgggcgt tccggcctcg gtggtcagtg ggctggcgga cggcagttat cctgtcagcg
     4021 tctcggtaac cgacaaagcc ggaaacacgg gcagccagtc actgactgtc acggtcaata
     4081 ccgcagcgcc ccttatcggc attaacagca ttgcgggcga tgatgtgatt aacgccagcg
     4141 aaaaaggggc cgatctccag attaccggca ccagcgatca gccagtgggt accgccatca
     4201 ccgtgacgct caacgggcaa aactatgcgg cgacgaccga tgcctccggc aactggagcg
     4261 tcaccgttcc ggcatcggcg gtgacagcat taggccaggc caactacacg gtaacggcgg
     4321 cggtaaccag cagtatcggc aacagcgcca ctgccagcca taacgtgctg gtcgacagtg
     4381 cgctccccgg cgtgaccatt aacccggtgg caaccgacga tattattaac gccgccgaag
     4441 cgggcgtggc gcaaaccatc agcgggcagg tgactggagc cgccgttggc gacacggtaa
     4501 ccgtgacgct gggcggcaat acctacacca ccaccgttca ggccaattta agctggagcg
     4561 tgagcgttct ggcggcagat attcaggcgc tgggcaatgg cgatttaacg gtgagtgcct
     4621 cagtcaccaa tcaaaacggc aacaccggca gcggcacgcg ggatatcacc atcgacgcca
     4681 atctgcccgg cctgcgggtc gatacggtgg cgggcgatga tgtggtcaat atcatcgaac
     4741 acgggcaggc gctggttgtc accggcagca gttcggggct ggcagaaggc acgccgctta
     4801 ccgtcacgat taataatgtg gaatacacca ccgcggtgca ggccgatggt agctggagcg
     4861 tgggcgtcac tgcggcgcag gttagcacct ggcctgcggg gacggttaat attgctgtat
     4921 ccggggaaag tagcgccgga aactcggtga gcattacgca tccggtgacg gtggatctca
     4981 ctccggcagc gatcaccatc aacaccatcg ccacggacga tgtgattaac gccgcagaaa
     5041 aaggcgctga tctaaccctt tccggcacca ccactaacgt agaacccggt caaaccgtca
     5101 ccgtcacctt tggcgggaaa aattacactg ccagcgtagc gagcgatggt agctggactg
     5161 ccaccgtacc cgccgccgat ctggcgtcat tacccgaggg cagcgcctcc gcacaggcca
     5221 gcgtcagcaa tatcaacggc aatagcgcct cggcggtgca caactacagc gtcgacagca
     5281 gcgcgccaac catcattatc aataccgtcg ccagcgacaa tatcgtcaac gccagcgaag
     5341 ccgatgcggg tgtgacggtg agcggcagca ccaccgctga agcggggcag attgttacgg
     5401 taacgctcaa tagcccgacc gtgcagacct atcaggcaac ggtgcaggcg gacggcagct
     5461 ggagcatcaa tattccggcg gcagatcttg aggcattgac cgatggcagc cacaccctga
     5521 ccgccacagt caatgacaaa gcgggcaacc cggcgagcac cacgcataat ctggcggtgg
     5581 atctcaccgt tccggtactc accatcaaca ccattgcggg cgatgacatt ataaacgcca
     5641 ccgaacacgg gcaggcgctg gtgatttccg gttccagcac cggcggagaa gcgggggatg
     5701 tcgtcaccgt cacgctaaac agtaaaacct atacaacaac cctggatgcc tccggcaact
     5761 ggagcgtcgg cgttccggcg gcggatgtca ccgcgctggg tagcggcccg caaaccgtga
     5821 ctgcgaccgt taccgatgcc gccggtaaca gcgatagtga gacgcacacg gtcaccgtta
     5881 accttaccgc gccgaccatt ggcattaaca ccatcgccac cgatgacgtg attaacgcca
     5941 ccgaaaaagg cgcggatctg caaatttccg gcaccagcaa tcagccagca ggcaccacaa
     6001 ttacgctgac gctgaacggg caaaattaca ctgccaccac agatgcttcc ggcaactgga
     6061 gcaccacagt gccagcttcc gccgtaggtg cgctgggcga agccagctac acggtgacgg
     6121 cgaacgtcac cgacagcgca ggcaacagca attccgccag ccataatgtg caggttaata
     6181 ccgcgctgcc tggcgtaact attaatccgg tggcgagcga cgatattatt aacgctgccg
     6241 aatcgggcgt agcgcagacc atcagcggcc aggtcacggg cgcggcggcg ggcgatacgg
     6301 ttaccgtaac gcttggcggg aaaacctaca ccgctaccgt gcaggggaat ctaaactgga
     6361 gcgtggacgt tccggcggcg gatattcagg ccatcggtaa tggcgatctg acggttaacg
     6421 cctcggtaac caacggcgtt ggcaataccg gcagcggttc gcgcgagatc gtgatagacg
     6481 ccaacctgcc aggtctgcgc gtcgataccg tggcgggcga tgatgtggtt aacagcatcg
     6541 aacacggtca ggcactggtg ataactggta gcagcagcgg gctggcggcg ggcgctgcgc
     6601 tgacggtggt gatcaataac gtcacctatg gcgcgacagt attagccgat ggcacatgga
     6661 gccttggcgt tccggcggca aatgtaggca actggcctgc gggtacggtg aatatcacgg
     6721 tttcaggtgc tactagcgca ggaaacccgg tcaccatcac ccatccagtc accgtcgatc
     6781 tggcagctgt ggcgatctcc attaacaccg tttccggcga cgatgtgatt aatgccgctg
     6841 aaaaaagtgc agatttaacc ctttccggca gcacctccgg cgtggaagtg gggcaaacgg
     6901 tcaccgttac ctttggcggg aaaacctaca ccgccaccgt agcaggcgat ggcagttgga
     6961 caaccagcgt acctgccgcc gatctcgccg cattacgcga cggcgacgcc acggtacagg
     7021 cgagcgtcag cactattaac ggcaacacgg cttcggcgac ccacgcctac agcgtcgatg
     7081 ccacggcccc gacgcttgcc attaacacca tcgccaccga cgatattctg aacgccgccg
     7141 aggcgggcaa tccgctaacc atcagcggca gcagcaccgc cgaagcgggg cagacggtga
     7201 ctgtcaccct taatggtgtg acttacaccg gtaccgttca ggcggacggc agctggagtg
     7261 tcagcgtacc aacggcggat ctcagcaatc tgaccgccag ccagtacacc gttagcgcct
     7321 cggtaagcga taaagcgggt aacccggcgt ccgctaacca cgggctggcg gtggatctca
     7381 ccgtaccggt gctgactatt aacaccgtct ccggcgatga cattattaac gctaccgaac
     7441 acggacaggc gctggtgatc tccggttcca gcactggcgg ggaagcgggc gatgtcatca
     7501 ccgtcacgct aaacagtaaa acctacacca ccacgctgga cgcttccggc aactggagcg
     7561 tcggcgttcc gctgtcagat gtcaccgcac ttggcagcgg cccgcaaacc atcacggcca
     7621 cgattacgga tatcgcgggt aatagcgatg atgctagccg cacggtcacc gtaaatctca
     7681 ccgcgccaac cattggcatc aacaccatcg ccaccgatga cgtgattaac gccacggaaa
     7741 aaggcgcgga tctgcaaatt accggcacca gtaatcagcc ggtgggcacc accattacgg
     7801 tgacgctgaa cgggcaaaat tacactgcta caactgattc ttccggcaac tggagcgcca
     7861 cggtgccagc gtcagcggtt agcacgctgg gtgaaactaa ctacacggta accgcgaacg
     7921 tcaccgacag cgcaggtaac agcaattccg ccagtcataa cgtgctggtc aacagcgcct
     7981 tgcctgccgt caccattaac gcggtggcga ccgacgatat tattaacgct gccgaagcgg
     8041 gcaatgcgca aaccatcagc gggcaggtga cgggtgccgc ggcgggcgac acggttaccg
     8101 ttacgctggg cggcaacacc tacaccgcta ccgtacaggc taatttaagc tggagcgtta
     8161 gcgtgccagc ggcggatatt caggcgctgg gcaacggcga cctgacaatt aatgcctcgg
     8221 tcactaacgt cgtcggcaac accggcagcg gctcgcgcga tatcacgatc gacgccaatc
     8281 tgcctggctt acgggtcgat accgtggcgg gcgatgatgt gattaacagc attgagcaca
     8341 atcaggcgct ggtgatcacc ggtagcagca ccgggctgac ggcgggaacg gcgttaacgg
     8401 tcgagattaa caacgttact tatggcgcga cggtgttagc cgacggcaca tggaacctcg
     8461 gtgttccggc ggcagacgtc agcaactggc ctgcgggtac ggtggatatc acggtaagcg
     8521 gcaccaacag cgccggaaca acctccacca ttacccatcc ggttaccgtc gatctgtcgg
     8581 cagtcgccat caccattaac actctctctg gtgatgatgt gatcaacgcc gtcgaaaaag
     8641 gcgaaacact ggttgtgagc ggcagcacca gcggtgtgga agccgggcag acggtgaccg
     8701 tcatctttgg cggcaaaaat tacaccacca ccgtggaagc caacggcagc tggacggtga
     8761 atgtaccgcc tgccgatctc gctgcgctgc cggacggcgc gggcaacgtg caggcgagcg
     8821 tcagcaacat caacggcaat aacgcccagg cggatcgtac gtacagcgtc gatgccaccg
     8881 cgccacttgt gaccatcaac accatcgcca gcgacgatat ccttaacgtg agcgaagctg
     8941 gcgcggggat caccatcagc ggtaccacca cggcgcaggc cgggcagacg ctcaccgtca
     9001 cgctcaataa caacacgtac cagaccaccg ttcaggcgga tggcacctgg agcgtgaatg
     9061 ttccggcagc ggatttaagc ggattaaccg ccagcagtta caccgtgacc gcctcggtga
     9121 gcgacaaagc gggtaacccg gaaagcgccg accacgcgct ggcggtggac gttaccgcgc
     9181 cggatctcac cataaacacc gtttcgggcg atgacatcat caacgccatc gaacacgggc
     9241 aggcactggt cgtcagcggc atcagtacgg gcgcggaacc cggcgatgta gtgaccgtta
     9301 cgctgaacgg taaaaactac accaccacgc tggacgcctc cggtaactgg agtgtgggta
     9361 ttccggctgc ggatgtcacg gcgctggcaa ccggaagcca gacgatcact gccagcctga
     9421 gcgatcgcgc gggcaacagc gacagcacta cccataatgt gaccgttgat ctcagcggcc
     9481 cgacgctgac catcagtatc gtctccggtg atgacattat taataatgcg gagaaaaccc
     9541 aggatctaat catcagcggc ggctccagcg gcctggcgac cggcaccacg gtcaccgtga
     9601 tgttgaacgg cctggcgtac agcgccacca ccgacggttc cggcaactgg agcgttaccg
     9661 tgcccgcttc ggcggtcggc gcactgggcg aggcggtgta tcagattagc gccagcgcta
     9721 ccgacagcgc gggcaacagc ggcatcacca cccatacggt gaatgttgaa tcgctcctgc
     9781 ctggggtgat cattaatacc gtggcgggcg atgacattat caacgcggct gaaatcgctg
     9841 tggcgcagac catcagcggg caggtcacgg gaacggcggc tgcggggaac acggtgacgg
     9901 tcatcattgg cggcaaccaa tacaccgcca ccgtacagcc agatttaagc tggagcgtca
     9961 gcgtacctgc gaacgtgttg caggcgctgg gcaacggtga gttgaccatc agcgcctcgg
    10021 tgaccaattc cgcgaataat accggcaccg caacgcacga catcgtaata gacgccaacc
    10081 tgccaggtct gcgcgtcgat accgtggcag gcgatgatgt gattaacagc atcgagcaca
    10141 ctcaggcgtt ggtgatcacc ggtagcagta gcggactggc ggcgggcgcg gcgttgacgg
    10201 tggttatcaa tagcgtcacc tacggcgcaa cggttttagc ggatggtagc tggagcgttg
    10261 gtgttccggc ggcagatgtc acaaactggc ctgcggggac ggtcaatatt gccgtctctg
    10321 gcaccaacac cgccggaacg acaaccagta ttacccatcc ggtgaccgtc gatctcgcag
    10381 ccgtggcgat caccatcaac accctttcca ctgacgatgt gattaacgcc gccgagaaag
    10441 gctctgattt gcagctctcc ggcaccacct ccggcgtgga agcggggcaa accatcaccg
    10501 ttatcttcgg tggtaaaagc tacaccacca cggttgccac agataatagc tgggagctga
    10561 cgatccccgc cgccgatctc gcaaccttgc cggacggcgc agcaaacgtt caggccagcg
    10621 tcagcaacgt cgcagggaac agcgcccagg caacgcatgc ttacagcgtc gatgccactg
    10681 cgccctccgt caccatcaac accatcgcca gcgacgatat tcttaacgcc gccgaagcgg
    10741 gatctgcgct gatcatcagc ggcaccagca cggcggaagc cgggcagacg gtaaccgtga
    10801 cgctaaacgg cgttaattac agcggcaatg tgcaggcaga cgggagctgg agcgtcagcg
    10861 taccaaccgg cgatttagct agccttaccg ccagctcgta taccgttaac gcctcggtca
    10921 gcgacaaagc cggaaatccg gcctcggcga cgcataacct gacggtggat ctcgttgcgc
    10981 cggtcgttac catcaacacg gtagcgggcg atgacatcat taacgccacg gaacacggac
    11041 aggcgcagat tatcagcggc tcggcaacgg gcgcgactac cggtaatacg gtttccgtga
    11101 cgattggcac gaccacctat accaccgtgc tggacgccaa cggcaactgg agcatcggcg
    11161 tgcctgccag cgtgatttcc gcactggcgc agggcgatgt aaccattacc gccacggtta
    11221 ccgactccgc aggcaacagt ggcacggcct cgcacactgt cagcgtggcg ctcggtgcgc
    11281 cggtactcgg cattaacacc atcgccgtcg atgacatcat caacgccacg gagaaaggcg
    11341 cggatctggc gattaccggt agcagcaacc agcctgcggg cacgcagatt accgttacgc
    11401 tcaacgggca aaattacacc accacagccg atgcttccgg gaactggagc gtgaccgttc
    11461 cggcgtctgc ggttggtacg cttggtgaag cgacttatac cgtgaccgcc tccgccactg
    11521 acgccgatgg caacagcggt tccgccagcc ataacgtaca ggttaatacc gcgctgccgg
    11581 gcgtcaccat taacgtggtg gcaacggacg atattattaa cgccgccgaa gcgggcgtgg
    11641 accagaccat cagcgggcag gtgacgggtg cggcggcagg cgacacggtg accgtcacgc
    11701 tcggcggagc gacttacacc gctacggtgc aggcgaattt aagctggagc gtcgatgttc
    11761 cggcctccgc gctacaggcg ctgggtaacg gtgagttaac cgttaacgcc tcggtcacca
    11821 acgccgttgg caacaccggc agcggcacgc gcgatatcac catcgatgcc agtctgcctg
    11881 gcctgcgggt ggataccgtc gcgggcgatg acgtggtcaa cattatcgag cacgggcagg
    11941 cgctggtgat taccggcagc agctccgatc tggcagcggg cagcaacgtc acgctgacca
    12001 taaacgggca aacctatgtt gcggcagtgc tggcggatgg ctcatggagc gtcggtgttc
    12061 cggcggcaga tgtcagcgcc tggcctgcgg ggacggtcac gattacagcc agcggcaaca
    12121 ccaccgccgg aaacccggtc agcgttacgc atccggtgac tgtcgatctc acggcggtgg
    12181 cggtgagcat caacaccatt accgtcgatg atgtgattaa cgccgctgaa aaaggcgcgg
    12241 cgttaacgct ctccggcagc acctctggcg tcgaagccgg acaaacggtt accgtcacct
    12301 ttggcggcaa aacttacagc gccacggtgg cggctaacgg ttcctggagt accacggttc
    12361 cggcggcaga tatggcggcc ctgcgtgatg gcgatgccag cgcacaggcc agcgtcagca
    12421 atgttaacgg caacagcgcc accactactc acgcatacag cgtcgatgcc agcgcgccaa
    12481 cggtgaccat taacaccatt gcgggcgacg acattcttaa cgccgccgaa gccggagcgg
    12541 cgctgaccat cactggtagc agcacggcgg aagcggggca gacggtgacc gtcaccctca
    12601 atggcacaaa ctataccggc accgtacaga cggacggcag ctggagcgtc agcgtaccgc
    12661 cagccgactt aagcgccctg accgccagca actacaccgt tagcgcggcg gtgaacgaca
    12721 aagccggaaa cccggcctca gttaatcaca acctgacggt ggatacttct gttccggtcg
    12781 tcaccatcaa cacggtggca ggcgatgatg tgatcaacgc gacggaacac gcccaggcac
    12841 agatcatcag cggctccgcc accggtgcgg caaccggtag caccgtgacg gtgactatcg
    12901 gcacaaatac tttcaccacg gtgctggatg ccagcggcaa ctggagcgtc ggcgtcccgg
    12961 caagcgtcgt ctcggcgctg gcaaacggca cggtgaccat taacgccagc gtcactgatg
    13021 ccggaggaaa cagcggcagc accacccatc aggtgatggt caataccggg ctgccgacca
    13081 ttacctttaa cgctatcagc ggcgataacg tcctgaacgc cgatgaaaaa ggccagccgt
    13141 tgaccatcag cggcggcagt acggggctgg cgacgggcgc gcaggtaacc gtcacgctca
    13201 acggtcacaa ctacagcgcc accaccgacg cgtcgggcaa ctggacctta accgtgccgg
    13261 tgagcgatct ggcggcatta ggtcaggcca actatacggt cagcgccagc gccaccagtg
    13321 cggcgggcaa caccgccagc agccaggcga atttactggt cgacagcggc ctgccgggcg
    13381 tcaccatcaa caccgtggca ggcgacgata ttatcaacgc cgccgaagcg ggggccgatc
    13441 aaaccatcag cggggtggtg actcgcgccg ccgctggcga tacggtcacc gtgacgctgg
    13501 gcgggaacac ttacacagcg gaggttcagc ccgatttaag ctggagcgtc accgttccga
    13561 cagacgattt acaggcgttg ggcaatggtg atttgaccat taccgcctcg gtcaccaacg
    13621 ctaatggcaa tacgggcagc ggcacgcggg atatcaccat tgatgccaac ctgccagggc
    13681 tgcgggtgga taccgtggcg ggcgatgata tcgtcaacag cattgaacac ggacaggcgc
    13741 tggtgatcac cggcggcagt agtggcctga atgcaggtgc tgtgctgacg gttaccatca
    13801 acagtgtggc gtattccgcc acggtacagg cggacggaag ctggagcgtt ggcattccag
    13861 cggcaaacgt cagcgcatgg cctgcggggc cgttagccgt tgacgtggca ggacagagca
    13921 gcgccggaaa tccggtcagc gtcagccatc cgttcaccgt cgatttaacg gcggtggcaa
    13981 tcagcatcaa caccgttgcc agcgacgacg tgattaacgc cgccgaaaaa ggcaccgacc
    14041 tgaccctttc tggcagcacc agcgggattg aaagcgggca aaccgttacc gtcacctttg
    14101 gcggcaaaac ctacaccgca agcgtcgccg ccaacggaag ctggagcgtg aacgttccgg
    14161 cggcagatct ggcaactctg ccagacggcg cggcgaacgt gcaggccagc gtcagcagcg
    14221 cgagcggtaa cagtgcctcg gcgacccatg cgtataacgt tgacgccagc gcgccgacgc
    14281 tcaccattaa tactatcgcc agcgacgata ttcttaacgc cacagaggcc ggaagcccgc
    14341 tcactatcag cggcaccagc accgccgaaa ccgggcagac ggtcaccgtc acccttaacg
    14401 gcgcaaccta cagcggtaat gtgcaggcag acggtagctg gagcgtcagc gttcccactt
    14461 cagccttagg cgcgctgacc gcaagcaatt acaccgtcag cgccacggtc aacgacaaag
    14521 cgggcaaccc cggcagcgcc agccacaatc tggcggtaga taccaccgcg ccggttctca
    14581 ccattaacac cgtggcgggc gatgacatca tcaacgacgc cgaacacgcc caggcgctgg
    14641 tgatttccgg caccagcacg ggcggggaag cgggcgatgt ggtgagcgtc gtgctcaacg
    14701 gcaaaaccta caccaccacc ctggatgcct ccggcaactg gagcgttggc gtaccggcgg
    14761 cggatgttac ggcgctgggt agtggtgcac agaccatcac cgccagcgtc agcgatcggg
    14821 cgggcaacag cgacgacgcc agccgcaccg tgaccgtcag cctcagcgcg ccagtgatta
    14881 gcatcaacac catcgcgggc gatgatgtga tcaacgcgac ggaaaaagga tccgatctgg
    14941 cgctttccgg caccagcgat cagcctgcgg gtacggcgat caccgtcacg ctgaacggac
    15001 aaaactacag cgccactacc gacgcctccg gcaactggag cgtaaccgtg cccgcctcgg
    15061 cggtcagtgc gctgggcgaa gcgacctaca gcgtgacggc gagcgtcacc aatgctcagg
    15121 gtaacagcag caccgccagc cataacgtgc aggttaatac cgcgctgccg ggcatcacca
    15181 ttaatccggt ggcgacggac gatattatca acgcttcaga ggcaggcagc gcgcaaacta
    15241 tcagcggcca ggtaaccggg gcggcggcgg gcagcaccgt caccgttgaa ctgggcggta
    15301 aaacttacac cgccaccgtc caggctgatt taagctggaa tgtgagcgta cctgccgccg
    15361 actggcaggc gctgggtaac ggtgagttaa cggttaatgc ctcggtgacc aacgccgttg
    15421 gcaacaccgg tagcggcacg cgcgatatca ccatcgatgc cagtctgcct ggcctgcggg
    15481 tggataccgt cgcgggtgat gatgtggtca atatcatcga acacgctcag gcgcaggtga
    15541 tcaccggcag cagctccggc tttgccgcag gcacggcgct gaccgtggtg attaataacc
    15601 aaacttacgc cgccacggtg ttggcgaatg gtacctggag cgtcggcgta ccggcgacgg
    15661 atgtcagtaa ctggcctgcg ggaacgctga atattaccgt tagcggggcg aacagtgccg
    15721 gaacgcaaac cagcattacc catccgctga ctgttgatct cacgaccgtc gccgtcagca
    15781 tgaacagcat caccagcgat gatgtgatta acgtcgccga aaaaggcgcg gcgctaacgc
    15841 tctccggcag cacgtctggc gtcgaagcgg ggcaaaccgt caccgtcacc tttggcggca
    15901 aaaactacac caccacggtg gcggctaacg gtagctggag caccaccgtt ccggctgcgg
    15961 atttggcggc gctgcgtgac ggcgatgcca gcgcccaggt gcgggtgact aacgtcaacg
    16021 gcaacagcgc cacggcaacg cacgaataca gcgtcgatag cgccgcgcca acggtcacca
    16081 tcaacaccat tgccagcgat aacatcatca acgccagcga agcggcggcg ggcgtaacgg
    16141 tgtccggcac cagcacggcg caaaccgggc agacgctcac cgtcacgctt aacggcacta
    16201 actaccagac cacggtgcag gcagacggca gctggagttt aacgctgccc gccagcgact
    16261 taaccgcact tgctaataac ggctacaccc tgaccgccac ggtcagcgat ctggcgggta
    16321 accctggcag cgccagcaaa ggcgtgaccg tcgataccac tgcgccggtg atcagtttta
    16381 acaccgtggc gggcgatgat gtgattaaca acgtcgaaca cattcaggcg cagattatca
    16441 gcggcaccgc cacgggcgcg gtggcgggcg accgcctggt ggtgaccatc gccgggcagc
    16501 agtatgtcac cagcaccgat gccagcggca actggagcgt cggcgtgcct gccagcgtga
    16561 tttccggcct tgcggatggc acggtgacca tcagcgccac cattaccgat agcgcaggca
    16621 acagcagcac gcagacgcac aacgtgcagg tgaacacggc ggcagtgtcg ctttcggtca
    16681 gcaccatcag cggcgataat attattaacg ccgccgaagc gggcagtgcg ctgaccctga
    16741 gcggcactgg cactaatttc gcgacaggta cagtggtgac cgtgttgctt aacggcaaag
    16801 gctacagcgc caccattcag agcaacggga gctggagcgt gaacgtgcct gcggcggatg
    16861 ttgcggcgct cagtgatggc accagctaca cggttagcgc ctccgctcag gacagtgccg
    16921 gaaacagcgc cacggcctcg cgcagcgtgg ccgtggatct caccgcgccg gttatcagta
    16981 ttaacaccgt ttcgacggac gaccgactca acgctgccga acagcagcag ccgttaaccc
    17041 ttaacggctc gaccagcgcg gaagtggggc agacggtcac cgtgaccttt ggcggcaaaa
    17101 cctataccgc tacggttgcc gccaatggta cctgggcgct gaacgtgcct gcagcggatc
    17161 tggccgcgct cgggcagggc gcgcagacca ttaccgccag cgtgaacgat cgcgccggta
    17221 accccggaca ggccacgcac gccctgaccg tcgataccgt tgcgccaacg gtcaccatcg
    17281 ccacggtggc gggtgacgat attatcaaca acgccgagca gcttgccggg cagaccatta
    17341 gcggtaccac caccgccgaa gtgggccaga cggtgaccgt tacctttaac gggcaaacct
    17401 ggagcgcaac ggtcggcagc ggcggaagct ggtcggtgtt tattccggcg cagcagtttg
    17461 ccggattaag cgacggcagc tacaccatta gcgcgacggt tagcgatcag gccggaaacc
    17521 ccggcagcgc cagccgtggc gtgacgctga acggcgacgt gcccaccgtc accatcaaca
    17581 cctttgcggg cgatgatgtg gtgaatgcag cggaacacgg ctcgtcgctg gttatcagcg
    17641 gcaccaccac cgccccagtc gggcagacgc tgaccctgac cttaaacggc aaaacctaca
    17701 ccaccacggt gcagacgggc ggtagctgga gctacaccct cggcagcgcc gatgtcaccg
    17761 cgctggcgga cggcaacgcc tacgtgatta acgcctcggt gagcaacgct atcggcaaca
    17821 ccggcagcag caatcacacc attaccgtcg atctcagcgc cccggcgatg ggtattaata
    17881 tcgattccct gcaagccgac actggcctta gcgccagcga ctttatcacc agcgtcagtc
    17941 cggtggtggt caacggctcg ctcaccgccg cgcttgccag taacgagacg gcgcaaatca
    18001 gtatcgacgg cggcgtaacc tggatcacgc tcaccgttac cggcacgacc tggcactata
    18061 acgacagccg cacgctgacc gatggcaact atctctatca ggtgcgggtg attgacgcag
    18121 cgggcaacgt tggcgcgacc gacagccaga atgtggtgat cgacactacc gcgccagatc
    18181 ccgcggtgaa aaccatcgcc atcagcgcga tcaccaccga taccggattg atcactaacg
    18241 attttgtcac cagcgacacg acgcttgcgg tgagcggcac gctgggcgcg gcgctttctg
    18301 ccggtgagtt cgcgcaaatc agtatcgacg gcggcaccac ctggcagaat ctttcggtaa
    18361 gcggtttgac ctggacctgg cttgacgggc gtacgctgac ggatggcaac tacaactatc
    18421 aggtacgggt gatcgacacc gccggaaata tcggcgccac cgcaagccag atagtcaccg
    18481 tggataccac cgcgccgctg gcgagcaaaa ccatcgccat tgcgggcatc agcgatgaca
    18541 ccgggttaag cagcagcgat tttgttactc gcgacaccac gctgaccgtg cgcggcacgc
    18601 tcggcgcggc gcttgccgcc gatgaacggg cgcaaatcag tctcgacggc ggtgtcacct
    18661 ggactacgtt aaccgtcgtt ggcaccagct ggagctatgc cgatggtcgc acgcttactg
    18721 acggcacctg gaactacacg gtgcgggtgg tggatctggc ggggaacgtt gggcagaccg
    18781 caacgcaaaa cgtggtggtc gacaccacca gcccggaagc ggcgaaaagt atcaccatta
    18841 ccggtatcag cgatgacacc ggagccagca gcagcgattt tattaccagc gacaccacgc
    18901 ttaccgtgcg cggcgtgttg ggcgcggcgc tcggcgctaa tgagttcgca caaatcagta
    18961 ccgacaacgg cgcaacctgg gtgaacgtga cgctagcagc agacggtctg aactggagtt
    19021 acgttgacgg acgaaccctc accaacggca ccaccacctg gcaggtgcgg gtggtcgatc
    19081 tggcgggcaa cgttggcgca accggcagcc agtcggcgca gattgacacc gttaacccgg
    19141 cgcaggtgct caccatcgcc agcattagca ccgacacggg gagttcggca accgacttta
    19201 tcaccagtga taccacgctc acgctgaccg gttcgctggg ggcggggctt gccagcggcg
    19261 aagtggcaca gattagcctt gatagcggcg cgacctggat cacgctcacc accaacggta
    19321 cacagtggac ttacaccgac agccgcacgc tgaccgacgg cagctacgtt tatcaggtgc
    19381 gggtgctgga tctggcgggg aacaccggcc cggtggtatc gaaaacggtg gtggtcgata
    19441 cgattaaccc taccgccacg ccaaccattg tgtcgtatac cgatgatgtc gggcagcggc
    19501 aggggacatt aagcagttcg caggccaccg acgacactac gccgctgctg aacggtgtac
    19561 tctccgcgcc gcttgccagc ggtgaagtgg tttacctcta ccgtaacggg ctgctgttag
    19621 gtgcggtgac gatggtcggc gctctgaact ggacctacag cgacagcggg ctggtgagcg
    19681 gtgcctatac ctacagcgcg cgagtggtgg atctggcggg gaatatcacc tcctccagtg
    19741 attttgtcct gacggtcgat acctctattc cgaccacgct ggcgcaaatc accagccaga
    19801 ccacgcgcga tactacgccg attatcagcg gggtgatcac cgccgcgctt gccagtgggc
    19861 agtatgttga agtggtgatc aacggcaaaa cctacacctc tgaaccgggc ggcgcggtag
    19921 tggtcgatcc ggcgcacaac acctggtatg tacagttgcc ggataccgat gcgctgacag
    19981 tttccgcgac cgcctatacc gttactgcgc aggtaaaaag ttcggcgggt aacggcaata
    20041 acgccaatat tagcaacggc acggtgacgg ttaacgcggc gattgattac acaccgacct
    20101 ggaccaccgc cagcaaaacc accgcctggg ggttgaccta cggcctcgac tcgcacggga
    20161 tgtggacggt gctggcaaac cagcaggtaa tgcaatcgac tgacccactc acctggtcga
    20221 agaccgcgct gacgctgtat cagagcggca acaactacgc caccagctcc atcgccgatt
    20281 acgaccgtaa cggcacgggc gatctgttta tcacccgtga tgactacggt acaggctata
    20341 tcaacggctt taccaataac ggcgacggca ccttttccag cgctattcag gtcaccgtcg
    20401 gcaccctgac gtggtacggc tcgattgtgg catttgataa agagggcgac ggctatctcg
    20461 acttctggat tggcgacgcg ggcgggccgg actccaacac cttcctgtgg aacaacgcag
    20521 gtacgctggt aggtaactcc accacgtcga acagcggcgg tagcgccacg gtgggcgggg
    20581 cggtgacggg gtatctttcg ctcaacgaag gttctggcgt cgatctgaac aatgacggca
    20641 ggatcgacct ggttcagcac acctacaacc tgaacaacta ttacacgcta tcttcgctca
    20701 tcaaccaggg gaatgggacg tttgtctggg ggcagaacac caccaatacc ttcctgagcg
    20761 gggcgggcag tggcgctatg agcagcagcg tttccatgac ctgggccgat ttcgatggtg
    20821 acggcgatat ggatctcttc ctgcccgcca gccagggaag agctaactac ggctcgctgt
    20881 tattcaacac caacggcgta ctgggtagcc cggtggcggt gggcgcaacg gcaaccacct
    20941 acgccagcca gtttagcctg gcagtggact ggaaccatga cggtctgatg gatatcgccc
    21001 gtatcgccca gaccggacaa tcgtatcttt acaccaacgt tggtggtgcc agcaactgga
    21061 cacaatcagc cctcggcggc agccagagcg gtaccaccag cggcgtggcg gcaatggact
    21121 acgactggga cggcgcggtg gatgtgctgg tgaccaaaca gtcgggcagc gtgttcctga
    21181 tccgcaacac caacacggtg agctacggca cttcgctaca cctgcgcatc accgatccca
    21241 acggcattaa cgtctattac ggcaataccg tgaagctgta caactcggcg ggagtgctgg
    21301 tcgccacgca aatcatcaac ccgcagtcgg gtatgggggt taacgacacc tcggcgctgg
    21361 tcaacttcta cgggctgaat gccggagaaa cctacaacgc ggtgctgatc aaatccaccg
    21421 gcaccaccgc cagcaatatc gaccagacgg tcaacaccag ctggggcggt ttacaggcca
    21481 ccgatgccac tcacgcttac gatctcagcg ctgaagcggg taccgcgagc aacaacggca
    21541 agttcgtcgg caccggctat aacgacacct tcttcgccac cgcaggcacc gacacttacg
    21601 acggttccgg cggctgggtg tacagctccg gcaccggaac gtggctggcg aacggcggga
    21661 tggacgtggt tgatttccgg ctttcgacgg tgggcgtgac ggctaactta agcagcactg
    21721 ccgcgcaggc caccggtttt aacacctcga catttaccaa tatcgaaggc atttccggct
    21781 cgaattttaa cgacactctg accggcagca gcggcgataa ccaacttgaa gggcgcggcg
    21841 gcaacgacac gctcaacatc ggcaacggcg gccatgacac cttgctctat aaactgctca
    21901 acgccagcga cgccactggc ggcaacggtt cggatgtggt gaatggcttt acggtaggca
    21961 cctgggaagg cactgccgac accgatcgca ttgatatccg cgagttgttg cagggcagcg
    22021 gctacaccgg aaacggcaaa gccagctatg tcaacggcgt ggcgacgctg gatgcgcagg
    22081 ccggaaacat cggtgacttt gtcaaagtca cccagagcgg cagcgacacc atcgtgcaga
    22141 tcgaccgcga cggcacgggc ggcacttttg cgacaactaa cgtggtcacg ctgacgggcg
    22201 tgcacaccga cctcgccacc ttgctggcga atcatcagtt gatggtggtg tagataatga
    22261 aaaaaaatgc ccagtccgtg gaagcctggc tggaggcgat gatcgccgtg gcgcgctatt
    22321 atcggctcga tttttctcag gagaatgtgc gcgcgaccgt caactgggag cgcgacagta
    22381 agcgggagga gttactcacc gacatggcgc ggcagttggg aatggggtta cggctggtgg
    22441 agttttccgc cgattcgctt aacccctggc gtctgccgct gatcgcggtg tttgataatc
    22501 agcagattgg cgtgatcacc cgtcgcgaca atcatgacaa tatcagcgtt cagttcagcg
    22561 gtgacgaagg gctggaaacc acgcttaacg tcgcggatgt cgaggataaa atcgttgaac
    22621 tggcgctact gcgcccgctc agcgccatcc ctgacgcgcg ggtggatgac tacattcgcc
    22681 cgtatcaggc gaactggttc tggaacctct cgctaaaaga ctggcggcgc tatggcgata
    22741 ttatgctggc gtcactggtg gcgaacgtgc tggcgctggc agcgatgatt ttctcgatgc
    22801 aggtttacga tcgggtggtt cctgcgcagt cctatccgac gctatgggtg cttttcgccg
    22861 gggtgatgat ggcgatcctg tttgagttct gtatgcgcat ggtgcgtacg catctttctg
    22921 atgtgatcgg caagcgggcg gatctgcgta tttcggatcg cgtttttggt cacgcattac
    22981 ggctgaaaaa caacgtgcga tcgaaatcca ccggatcgtt tatctcgcag atccgcgaac
    23041 tggaatcagt gcgggagctg attacttcca ccaccatcgg cgcggtggcg gacctgccat
    23101 tcttcctgct gtttgtcttt attttgtgga tgatcggcgg ctggctggtg ctggtggttt
    23161 tactggcgct gccgttgctg gtgatccccg gcctgctggt gcaacgaccg ctggcgcggc
    23221 tggcgaacga agggatgcgc gagtcggcgg tacgtaacgc tacgctggtg gaagccgtgc
    23281 agtcgattga agatattaaa ctgctgcgcg ccgaacagcg ttttcagaat cagtggaacc
    23341 acaccaacga cgtggcgtct tccatcagca tgaagcaacg ttttctcacc ggtctgctgc
    23401 tcacctggac ccaggaagtg caatccatcg tctacgtggt ggtgctgctg gtgggctgct
    23461 ttatggtgat gaacggcgac atgaccaccg gcgcgctggt cgggacgacg atcctggctt
    23521 cgcgcaccat cgcgccgctg tcgcagatat ccggcgtgct ctcgcgctgg cagcaggcga
    23581 aagtagcacg taacggcctt gatgaactga tgaagcgccc cgtggatcag ccggaacatg
    23641 gcaaactggt gcataaagcg gtgctgcacg gtaattatca gttcagcaac gcggtgtttt
    23701 attacgacga agaagagaaa attgccgatg tggcgatcgg taaactcaac attcaggccg
    23761 gagagaaaat cgccattctc gggcgcaacg gcgcgggtaa atcgacgcta ctgcaaatgc
    23821 tcgccggaat gcgtatcgcc caacaggggc aggtgctgct ggataacatc agcatcgggc
    23881 agcttgatcc ggcagatctg cggcgagaca tggggctact cagccagacc gggcgactgt
    23941 tctttggttc gctgcgggaa aacctcacca tggggatgcc ggaagccagc gatgaagata
    24001 tcgaacgcgc gttaatcctc agcggagcgc tgccctttgt gcagaagcag aaaaacggcc
    24061 tcaactacat gattcaggaa gggggatttg gcctctctgg tggtcagcgg caaacgctgc
    24121 tgctggcgcg gctactcatc tctcaaccca atatcgtcct gctcgacgaa cccagcgcct
    24181 cgctggacga aatggcggaa gcgtatttaa tcgaacaact gaaacagtgg attgggcatc
    24241 gcacgctggt tatcgccact caccgcacgg cgatgctgca actggtggac cgcattatcg
    24301 tgatggatca ggggcggatt gtgatggatg gcgcgaaaga ggccattttg cgtgaacaga
    24361 gcgaacccac ggcgcgacgg gtggtgttgc aggagaagaa taaggggtct gctgcatgaa
    24421 cggacaactg caatacaacc gtggcctgac tgaaccccgc ttgccgcgtt cggcgctggc
    24481 ggtacgcgtt accgctgtga tgctgctctg ttttctcggc tgggcgtggt acttccagct
    24541 tgatgaagtc accaccggca gcggcacggt agaaccctcc gggcgcgaac aggtggtgca
    24601 gtcgctggaa ggcggaattc tgtatcatct cgacgtgaaa gttggcgata ttgtcgaaca
    24661 gggccagccg ctggcgcagc ttaaccgcac caaaacggaa tccgacgtcc aggaagcgat
    24721 gtcgcggctg tacgcggcgc tggcaacctc ggcgcggctg cgggcggaag tgagcaataa
    24781 accgctggtc ttccctgacg aactgaataa attccccgaa cttatcgagt cggaaacggc
    24841 gttgtacaac actcgccgcg acgggttgaa caaagccacc accgggctga cgcaggggat
    24901 tagcctggtc aaccgtgaac tggcgatgac ccagccgctg gtgaaacagg gggcggcgag
    24961 cagcgtcgaa gtgctgcgcc tgcaacgcca ggccaatgaa ctggaaaaca aactcagcga
    25021 cgtgcgcacg caatactacg ttcaggcgcg cgaagagtta gcaaaagcca atgcggaagt
    25081 ggaaacccag cgttcggtga tccgtggacg ggaagactct ctcaccaggc tcaactttac
    25141 cgcgccggtg cgaggcattg ttcaggatat tgatgtcacc acggttggcg gcgtcatcgc
    25201 ccccggtggt aaactgatga ccatcgtgcc gctggatgag caactgctga ttgaggcaaa
    25261 aatctccccg cgcgatgtgg cgtttatcca cccggggcaa aaatcgctgg tgaaaataac
    25321 tgcctacgat tactccatct acggcggcct gccgggagaa gtggcggtga tatcaccaga
    25381 caccgtgcag gatgaagtgc ggcgcgatgt ttattactat cgggtgtaca ttcgcacttt
    25441 cagcaaccat ctggaaaaca aaagcaaaca gcagtttccg atattccccg gtatggtggc
    25501 gacggtcgat atccgcaccg gcaaaaaaag cgtgctggat tatctgttga aaccctttaa
    25561 caaagcacag gaggcgttgc gcgaacgatg aacattagcg atgtagcaaa aattaccggc
    25621 ctgaccagca aagccattcg cttctatgaa gagaaggggc tggtgacgcc gccgatgcgc
    25681 agcgaaaacg gttatcgtac ctacacgcag cagcatctca acgaactgac cttactgcgc
    25741 caggcgcgcc aggtgggctt taacctggaa gagagcggcg aactggtgaa tctgtttaac
    25801 gacccgcagc gccacagcgc tgatgtaaaa cggcgcacgc tggagaaagt ggcggagatc
    25861 gaacggcaca ttgaggaact gcaatctatg cgcaaccagc tgctggcgct ggcgaatgcc
    25921 tgccctggcg atgaaagcgc cgactgccca attatcgaaa atctctccgg ctgctgccat
    25981 catcgggcgg ggtgattagg gcttacggaa atagtcgtcc attattttcg tcagttccgg
    26041 acgttcgccg tcatcgttga tggcaaaatc cagaaaaaag gcatctgctt gcggcgttcg
    26101 ggtttgcgcc attagccgga aggtgagggg gcgataaatg gcgtataccg gatgactgat
    26161 aacgactcgg gcaaagtcaa caaaaagcgg cgagtgtgtg aaaaacagcg cctgcaaaat
    26221 tacatcagcc ttttcatcct cccccaaacc ttgttggatt atgcgcttaa tacgtgtttc
    26281 gctcaaattc ccggcctcaa caatttgcca gacttgatcc agtgaagggt aacggctgac
    26341 gctctgctgc aactcttctg accaggtttc gagtgggctt acgtgcttat cagggccgaa
    26401 actatcccat gcgctgggcg gcaatttttt ccacgcggtg agttttggga tgcggtattg
    26461 cagctctgtt ttgtgcagaa attccccgcc cggaaatacc agggtttgct gctcatcatt
    26521 aaaacgagtt gccgtgctga gcaccagggt tttgagcggt cgaccttcca gcgcggcggc
    26581 agtggcttta tggtgtccgt cgagtagaac gcatagatag gaatcagtga gataccaggc
    26641 cagagcgcgg gcgtccggtt tatcgcgata aaacgtcgca cgctcaggat taaaatgtga
    26701 tggcggctgg ccgggtagaa gaaagcaggg cgcggtgtca ctgaaagagc aatattcctg
    26761 atcccagacg gcaacggttg ctggattact ggagctatgg tccgttgccg cccaaaagaa
    26821 atgcccattg ccatctgtcg ggtaaagttc acgatcttcc agcagataat agccatcagc
    26881 caggcatgag aacagcggcg tcagcgtagc aaaggcgtcc gtcatgccag accacgggcg
    26941 attccagttt tcgagagcag acaaaaaccg ggcagctccc tgttcattat ccgcaaaacc
    27001 gctgtagaca aattccgcgc aggtggggca gtcggcctcg gaccagaaac gtatcaatgg
    27061 gacatcttgc cagcagagcg aacggataag cccgtaaggg tgcggatcgt aagtcacaca
    27121 ttgacgcaga aatcctttcc cggacttcac cgaaaaaatg acgcctgcag cggtattttt
    27181 cagtagacgt acttcaggtt ttcgacaggg gaaaagcatg gccactcctt agccaaaatt
    27241 tcagttccct gaactgtgcc agaaaattac gctggtgact attgctctgg aaatggaaac
    27301 ggtgccctta cccattaatc cccgtttgca ccgaaagaat atgctgcaaa ctttctacca
    27361 taatgcgccc cgattgaaat aagccttcct ggatttcatt gttaacattc acagcgtgaa
    27421 ccagggaggc taataacacc acttgcaaga aggcaaaaat aaacgcaccg ataatcataa
    27481 tatgcgtcag gggtattttc ctttgctcct tcattttaaa tagcccacaa actggccttc
    27541 tttcattaag gcgatttttc ctgtgtgatg ataaacaatg acgttataca gacctgactg
    27601 ataaacagtg gcatctttat tgtcttcatc aattgcacat aatgaatcat tgagataagc
    27661 gaagttttta ctgctcattc taaaggtcat gccaaatcct ttataaatgg ctttctttgc
    27721 cgcctgggca tgttgtgcgg cttctgcatt tttaaatgcc tgtgcttctg atgcacttaa
    27781 aatagccgtc tgacgctgct gttctgcgag ataacaggtg tcatgactga cgccctgcgc
    27841 ctggcaatct gcgatgcggg aagtgggtga actacagcca gccagtaata taacagccag
    27901 cagggaaaag gatttaatgg agctattcat cattgtta
//
LOCUS       DAZUCE010000040        27708 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZUCE010000040 DAZUCE010000000
VERSION     DAZUCE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.040"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..305)
                     /locus_tag="ACHY03_001492"
     CDS             complement(<1..305)
                     /locus_tag="ACHY03_001492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904739.1"
                     /translation="MDFTALVDNCAHFVESLIETEDPALWLTLCGRHCTAATHADLIP
                     PHFTESLTIDTLPAMPEQVISEPDFLCEYCLVRAVHRCRSLIRGVALLVWAMRLRYNS
                     "
     gene            587..826
                     /locus_tag="ACHY03_001493"
     CDS             587..826
                     /locus_tag="ACHY03_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HFS3904740.1"
                     /translation="MNEDEIYRHIRQALSSAPRNQYTVELHLQMIKYADKLEHITAKA
                     FCEGTGLSQSLGTEFSKMRNLTRRLKAAGLNTDLL"
     gene            866..3304
                     /locus_tag="ACHY03_001494"
     CDS             866..3304
                     /locus_tag="ACHY03_001494"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFS3904741.1"
                     /translation="MAIKKTELYSSLWASCDELRGGMDASQYKDYVLTLLFMKYVSDK
                     YKGDPYGMIVIPKGASFDDMVALKNDKEIGDKINKIIHKLAEENGLKGVIDEADFNDE
                     DKLGKGKEMIDRLSKLVGIFEGLNLSSNRAEGDDLLGDAYEYLMRHFATESGKSKGQF
                     YTPAEVSRILAKVIGITPDTPQDATVYDPTCGSGSLLLKVNDEARRGLSIFGQEMDNA
                     TSALARMNMILHNNATAKIWQGNTLSDPQWKEANGKLKAFDFAVANPPFSNKNWTNGL
                     TPKKDPFERFGWGIPPEKNGDYAFLLHIIKSLKSTGKGAVILPHGVLFRGNAEANIRE
                     NLIKQGYIKGVIGLPANLFYGTGIPACIIVIDKEHAHSRKGIFMIDASRGFIKDGNKN
                     RLRSRDIHRIVDVFNHQRTVPGYSRMVPSSEIAGNDYNLNIPRYIESGEPEDLHDLTA
                     HLQGGIPARDVDALQDYWRVFPALRNVLFADDRPGYCRAQVNAQQVKPTILAHQEFKD
                     FATRSLLPFKAWVKEASLEEIRKGDKPKALIHDISEMLLAQYANSELLNKYCVYQILM
                     DYWTDVMQDDVYAIMQDGWQAAAQIRELQPVKGKDGKNIWKETHDFEFTKRRYKADVL
                     PRSLVEIRCFPEQLDALNRAQERSEEASRQLVEFIEEQAGEEDLLAEAKNDKDKVTQK
                     LVNARLAQLKKTPADPDELAVLNRCLALIKAEASAKKALKAAQEALDRAVFKHYPTLD
                     EAAIKTLVVQDKWLATLQAGIKAEIERITQQLASRVKELEERYAEPLPALEAAVEALS
                     EKVAGHLRAMGLEW"
     gene            3304..4659
                     /locus_tag="ACHY03_001495"
     CDS             3304..4659
                     /locus_tag="ACHY03_001495"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS3904742.1"
                     /translation="MVDLMVNEQRSGTAGKDVPAGYKLTEVGVIPEDWVVISIDDCTT
                     KVGSGKTPTGGASVYIDSGRPFVRSQNVDWGRLDLADVVHITDETHNTFSGSEIQQDD
                     VLLNITGASIGRCAKADFRVIGGNVNQHVCIIRTEAKKLSPSLLVELINSNYGQNQID
                     SYQAGGNREGLNFSQVKNLKFAITPNSFEQTEIVRTLNDINAFTLALENLIAKKQAIK
                     AAAMQQLLTGKTRLPQFALRKDGSAKGYKKSELGAIPEDWVVTSIGQFTDCCAGGTPS
                     TKISAYWGGTHPWMSSGELHLKQVYAVADYITDEGLVNSSTKYVPKNSVLVGLAGQGK
                     TRGTVAINRIELCTNQSIAAIFPSKHHSTEFLFYNLDSRYEELRSLSTGDGGRGGLNL
                     TIIRKLHLAFPPKEEQTAIATILSDMDKEIQTLQQRLDKTRQLKQGMMQELLTGKTRL
                     I"
     gene            4671..5327
                     /gene="darT"
                     /locus_tag="ACHY03_001496"
     CDS             4671..5327
                     /gene="darT"
                     /locus_tag="ACHY03_001496"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system toxin DNA ADP-ribosyl
                     transferase DarT"
                     /protein_id="HFS3904743.1"
                     /translation="MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAEN
                     WINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTPFSPMLMNIHSGRGGIKQRPNEE
                     IVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLNSLDQIDWPILQARDFRRDPDD
                     PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS
                     "
     gene            5324..6391
                     /gene="darG"
                     /locus_tag="ACHY03_001497"
     CDS             5324..6391
                     /gene="darG"
                     /locus_tag="ACHY03_001497"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin DNA ADP-ribosyl
                     glycohydrolase DarG"
                     /protein_id="HFS3904744.1"
                     /translation="MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMK
                     VYALACKQKQVITGKMFITETGELMGPRWIVNFPTKQHWRADSRMEWIEDGLQDLRRF
                     LIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
                     GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAH
                     YYGPYAPNLNHLLNALDGTYLKAEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWL
                     PALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEGLHQWPAGKRWASRK
                     LRLFDNNNLQFAINRVMEFHC"
     gene            6410..9505
                     /locus_tag="ACHY03_001498"
     CDS             6410..9505
                     /locus_tag="ACHY03_001498"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015872650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS3904745.1"
                     /translation="MSTVGQRERATQQRLIQFFVEDLGYRHLSHRTGYTGNSNIDTNI
                     LSAWLKRRGVNDTLITRALRQLDSAAALGEGKKLYYANKEVYRLLRYGVKDKEGAGEQ
                     NQTVWLIDWANPQANDFAIADEVTVKGEYCKRPDLVLYVNGIALGVIELKRSSVHVSE
                     GIRQNLDNQKKTFIRDFFTTMQLVMAGNDTQGLRYGTIETPEKYYLEWKEDNPSDYTH
                     KLDFHLSRLCSKSRLLDIVHNYIAFDVGVKKLCRHNQFFGVEAAKQHIARREGGIIWH
                     TQGSGKSLTMVWLAKWIRENVPNSRVLIVTDRTELDEQIESVFMGVNEDIYRTSSGND
                     LIATLNHPNPWLICSLVHKFGCRSEAEDNAATDAFITELQQSLTKTFRAKGDLFVFVD
                     ECHRTQSGKLHNAMTAILPDALFIGFTGTPLMKKDKKKSVEVFGPYIHTYKFDEAVAD
                     GVVLDLRYEARDIDQYLTSEKKVDDWFEAKTRGLSNLARTQLKQKWGSMQKLLSSKSR
                     LEQIVNDILLDMDTRPRLMDGRGNAMLVCSSIYQACKVYEMFSQTELAGKVAIVTSYR
                     PDAASIKGEETGEGLTEKLSKHATYRKMLADYFEQSEEKAAARITEFEREVKQRFIEE
                     PGQMRLLIVVDKLLTGFDAPSATYLYIDKTMTDHTLFQAICRVNRLDGEDKEYGYIID
                     YKDLFRSLDKAITDYTTGAFDDYDRDDVAGLLKDRLEQAQLDLDSALEMVRSLCEPVK
                     TPRSLQDYQHYFCGKSGENQTSLNEKEALRLTFYQCVARLLRAYASIANEMPEAGYTA
                     EEVESIRAEVAEFEKLRYEIKLTSGDLLDMKRFEPAMRHLLDMYVRADGSEVLMDFEE
                     LSLIELIVEKGLAATVALPDDIRNNQDAMAETIENNVRKTIVDENPVNPKYYGRMSQL
                     LDELITLRRQNALNYQQYLERIRDLCKQVIRPEQTAGACYPATMDTQAKRAFYDNFGH
                     DEVLATRIDTTIRYTKRAEWIGDRFKEREIANALREETASYNIDIDEVIALARQQKEY
                     H"
     gene            9505..10221
                     /locus_tag="ACHY03_001499"
     CDS             9505..10221
                     /locus_tag="ACHY03_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFS3904746.1"
                     /translation="MPIMQIGSLHLLVNRKAIKNLHISVLPPDGKVRVSAPEHMTETA
                     IRMAVASRFRWIRKQQQDFARQPRQSEREMVSGECHYLWGRRYRLLVIERHGRHEVKV
                     AGNNKLQLYVQPGTRDENKALALSEFYRHELKQQVAKLLPEWQDRIGVPLTFWGIKKM
                     KTFWGTCNTTTKRIWLNLELIKKPPECLEYILVHELVHLLERHHNARFRLHMDRFIPD
                     WRERRDLLNNMPLAFEAWEY"
     gene            complement(10443..10568)
                     /locus_tag="ACHY03_001500"
     CDS             complement(10443..10568)
                     /locus_tag="ACHY03_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904747.1"
                     /translation="MHTNDRSLTMVDLFCCAGATARLVSEAGSEELRITVKEIGE"
     gene            complement(10661..11035)
                     /locus_tag="ACHY03_001501"
     CDS             complement(10661..11035)
                     /locus_tag="ACHY03_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904748.1"
                     /translation="MTLFDECREALSADFNIVEGLAQQEALGILNKYPLAKGSVIWSE
                     IWHSDYESFDELLCANSVKNDDMFVFADDASIPVFRSNLRLIAENIYDVTALSPKLFI
                     FNDEVIIQPLFPTDMFRLGIKK"
     gene            complement(11041..20586)
                     /gene="cdiA"
                     /locus_tag="ACHY03_001502"
     CDS             complement(11041..20586)
                     /gene="cdiA"
                     /locus_tag="ACHY03_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition effector tRNA
                     nuclease"
                     /protein_id="HFS3904749.1"
                     /translation="MHQPPVRFTYRLLSYLISTIIAGQPLLPAVGAVITPQNGTGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGNRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GRPVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATEVNAALHAKDLTVTA
                     GANRVTADGRVSALKGEGDVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGSLYARD
                     GDITLDASGRLTVNNSLATGAVTAKGQGVTLTGDHKAGGNLSVSSRSNIVLSNGTLNS
                     DKDLSLTAGGRITQQNEKLTAGRDVTLAAKNITQDTASQINAARDIVTVASDTLTTQG
                     QITAGQNLTASATTLTQDGILLAKGHAGLDAGTLNNSGAVQGASLTLGSTTLSNRGSL
                     LSGGPLTVNTRDFNQSGRTGAKGKVDITASGKLTSTGSLVSDDVLVLKAQDVTQNGVL
                     SGGKGLTVSAQALSSGKKSVTHSDAAMTLNVTTVALDGETSAGDTLRVQADKLSTAAG
                     AQLQSGKNLSINARDARLAGTQAAQQTMVVNASDKLTHSGKSSAPSLSLSAPELTSSG
                     VLVASALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSG
                     LITGDNGLTLNTASLSNPGKIIADTLNVRATTLDGDGLLQGAGALALAGDTLSQGRNG
                     RWLTAGDLSLRGKTLNTAGTTQGQNLTVQADRWANSGSVLATGNLTASATGQLTSTGD
                     IMSQGDTTLNAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTIRQNSVTNSGTLT
                     GIAALTLAARMASPQPALMNNGGSLLTSGDLTITAGSITSSGHWQGKRVLITADSLAN
                     SGAIQAADSLTARLTGELVSTAGSKVTSNGEMALSALNLSNSGQWVAKNLTLKANSLT
                     SAGDITGVDALTLTVNQTLNNHASGKLLSAGVLTLKADSVTNDGQLQGNTTTITAGQL
                     TNDGHLQGETLTLAASGGVNNRSGGVLMSRNALNVSTATLSNQGAIQGGGGVSLNATD
                     RLQNDGKILSGSNLTLTAQVLANTGSGLVQAATLLLDVVNTVNGGRVLATGSADVKGT
                     TLNNTGTLQGADLLVNYHTFSNSGTLLGTSGLGVKGSSLLQNGTGRLYSAGNLLLDAQ
                     DFSGQGQVVATGDVTLKLIAALTNYGTLAAGKTLSVTSQNAVTNGGVMQGDAMVLGAG
                     GAFTNNGTLTAGKGNSVFSAQRLFLNAPGSLQAGGDVSLNSRSDITISGFTGTAGSLT
                     MNVAGTLLNSALIYAGNNLKLFTDRLHNQHGDILAGNSLWVQRDSSGTANSEIINRSG
                     NIETTRGDITMNTAHLLNSWDAISASHEVIPGSSHGVISPVPENNRWWGVVRHDGVEY
                     LAVYWGEGATVPDEYRIRTGDTETVTVSASGHAARISGGADMHIRAGRLDNEASFILA
                     GGGMTLSGDTLNNQGWQEGTTGKETVWRLASGSLPKAWFTEPWYRVYRQVSPDATEAS
                     GTSPAGQYRAVISAAGDVSASFTTDTGNTTVMPRAGGAGNTITVPSLNSLTPPTVSQG
                     VSGEALRNESGTGITGPVWNDALPDTLKDIPGALSLSGASVSNYPLPSGNNGYFVPST
                     DPDSPYLITVNPKLDGLGKVDSSLFAGLYDLLRMQPGQAPRETDPAYTDEKQFLGSSY
                     ILDHLGLKPEKDYRFLGDAAFDTRYVSNVILNQTGSRYINGTGSDLAQMKYLMDNAAA
                     QQKALGLSFGVSLTAGQVAQLTRSILWWESVTINGQTVMVPKLYLSPEDITLHNGSVI
                     SGNNVQLAGGNITSSGSSINAQNDLLLDSTGSIDNLNAGLINAGGALNLKAIGDIGNI
                     SSVISGKTVSLESATGNISNLTRTEQWAMNNGYNHFSGTDTGPLAAVRATDSLFMGAA
                     GDISITGAAVSAGDSVLLAAGNDLNMNAIQAGERRRYGGSGWYETHAVAPTVTAGNSL
                     MLSAGRDVNSLAAGITAENSMAIRAGRDVNMAAESTGTGDHDSTFSMKTVHDSVRQQG
                     TDMTSGGDITVTAGRDITSVATAVTAKGDISVNAGHDIVLGTATESDYHYSESGETRN
                     RLLSHQTTRTITEDSVTREKGSLLSGNRVTVNAGNNLTVQGSDVVADRDVSLAAGNHV
                     DVLAATSTDTSWRFKETKKSGLTGTGGIGFTTGSSKTTHDRREAGTTQSQSASTIGST
                     AGNVSITAGKQVHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSGLTVAL
                     SGTVGSAINNAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGDPNATG
                     VSLSLTTQKSKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGSQLKAG
                     GNTSLDAANDVLLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVNAAKGS
                     EKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGNRIIADVGHDLLISSQQDTSKY
                     DSKQTSVAASGSFTFGSMTGSGYIAASRDKMKSRFDSVAEQTGMFAGDGGFDITVGRH
                     TQLDGAVIASTATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDKFQGNM
                     PGGMISAGGHSGHAEGTTQAAVAEGTITIRDRDNQKQNLANLSRDPVHANDSISPIFD
                     KEKEQRRLQTVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQRQEYL
                     AKLRDTQAYRNEMAKYGTGGDIQRGIQAATAALQGLAGGNLAGALAGTSAPELAHLLK
                     STEKDPAVNAIAHAILGGAVAAMQGNNVAAGAAGAATGELAARAIAGMLYPGVKQSDL
                     SEEQKQTISTLATVSAGLAGGLTGNSTASAAVGAQSGKNAVDNNYLSSKQIDAWSAEM
                     KSCQAKGGDCGGIIKKYEELSTAQQKQLISDCAASPATCQQKYGDVLTDSLAVKQAID
                     RALGEDIPIKMVYDLTATFAQQMQAEGVVATNKVSEALQEEYGLDEVQAGIVASAAAS
                     AFGGISKVKGNSQPKIEQILKPEKNWETARNKALDLVGNLGADSKPVIGRLEVSAGNG
                     KVIGRQSSDGKVGWRVDYDPEKGTHINIWDYSQGKGPGKAVKQVIPFEGNEKSFETIL
                     KQLNR"
     gene            complement(20599..22365)
                     /locus_tag="ACHY03_001503"
     CDS             complement(20599..22365)
                     /locus_tag="ACHY03_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFS3904750.1"
                     /translation="MQHRQDNLLANRTSFPGMVSGQYAFRIRTLSQVVRYFSLLPCLC
                     ILSFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPEMSAGTEGP
                     CFTVSRIVVSGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYIRRGYITSRA
                     FLTEQDLSGGVLHITVMEGRLQQIRAEGADLPARTLKMVFPGMEGKVLNLRDIEQGME
                     QINRLRTEPVQIEISPGDREGWSVVTLTASPEWPVTGSVGFDNSGQKNTGTGQLNGVL
                     SFNNPLGLADNWFVSGGRSSDFSVSHDARNFAAGVSLPYGYTLVDYTYSWSDYLSTID
                     NRGWRWRSTGDLQTHRLGLSHVLFRNGDMKTALTGGLQHRIIHNYLDDVLLQGSSRKL
                     TSFSVGLNHTHKFLGGVGTLNPVFTRGMPWFGAESDHGKRGDLPVNQFRKWSLSASFQ
                     RPVTDSVWWLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKEQYISGNNGGYLRNELSW
                     SLFSLPYVGTVRAVTALDGGWLHSDSDDPYSSGTLWGAAAGLSTTSGHVSGSFIAGLP
                     LVYPDWLAPDHLTVYWRVAVAF"
     gene            22964..23857
                     /locus_tag="ACHY03_001504"
     CDS             22964..23857
                     /locus_tag="ACHY03_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFS3904751.1"
                     /translation="MSQFDFTSPDPFARAISPFEEMAAYEALWSEKGATFKTIADKFR
                     AAPTGSLPSDFVSDSIIAAFKNRLKEIIARYDIDDLGIRVHGAGEYPEKLRDARNPIE
                     LLYYQGWWDLINTRSVAVVGSRRVSEEGAKRTRKVVKFLVEDGFTIVSGLAEGVDTNA
                     HQTALALGGKTIAVIGTPLSHHYPRQNSNLQKNIRDNHLLISQVPFHRYLEQDYRSNR
                     IFFPERNVTMSALTEATIIIEASETSGTLTQARAALAQGRKLFILESCFRNPSITWPA
                     RFEAQGAIRVRNYEDIRRHLR"
     gene            23854..24480
                     /locus_tag="ACHY03_001505"
     CDS             23854..24480
                     /locus_tag="ACHY03_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904752.1"
                     /translation="MTLRLTLIDELIRKQHHYLEDGDLCYCFGEYTARQGAAYSETNQ
                     LIINLKKGYERRAFPDYHYKKVAIEYVAQMLISNIGNLEEYTFVPVPPSKCRTDAAYD
                     DRMTEILRIAQTYEPWLDYRELVVQNVSTIASHTAANRPSPEAIMANYQLNEQLLEGC
                     RPWIVIFDDVLTAGSHFKAMKSLILQHIPEACILGLFVARTTRGAQII"
     gene            complement(24498..26858)
                     /locus_tag="ACHY03_001506"
     CDS             complement(24498..26858)
                     /locus_tag="ACHY03_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFS3904753.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPPISVQRVSV
                     LSTNEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTAGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRESGGSVFVDMNLQPYPDQWAFLVSVIPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNIAVTEPLIITLANQIYFEK
                     AQLPQALVNRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     ALSFLRYNNIAAELIDKRFAGTECNAVFTGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIARRKVNTLILVHRTELLKQWQERLAVFLQVGDSIGIIGGGKHKPCGNID
                     IAVVQSISRHGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAARPKESLHNLEVLTRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDDIASVMNSLKLSPFVLHSRLSKKKRTM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIVADGEGLSF"
     gene            complement(27013..27576)
                     /locus_tag="ACHY03_001507"
     CDS             complement(27013..27576)
                     /locus_tag="ACHY03_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS3904754.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHKKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDNSAVDNLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
BASE COUNT         6697 a         7366 c         7023 g         6622 t
ORIGIN      
        1 gagttgtagc gcaagcgcat ggcccaaact aataacgcta cgccccttat caaagagcgg
       61 caacgatgca cagcccgcac gaggcaatat tcgcacaaga aatcaggttc ggaaataacc
      121 tgctctggca tggcggggag agtatcaatg gtcaggcttt cggtaaaatg cggcgggatg
      181 aggtcggcgt gggttgcagc agtacaatgt cgcccgcaga gggtaagcca cagcgcggga
      241 tcctcggttt ctatcaggga ttcgacgaag tgggcgcagt tgtcgaccaa ggcggtgaaa
      301 tccatagtga cattataagg aaacgaacaa tagatcgagt aaatcgcagt cagggacaag
      361 gattgtagtc atagagaagt ctcctgtaac ggttttcagg aagctaatcc cgacgcctga
      421 ttttgcaggc gcggaaggag aagatactgc ggccattaat ccgccacaag gcatttacgt
      481 gcataaatcg cgcagtcaaa tccgactccc actgtagttc ttgaacctga taccacattt
      541 agctaattta gctaacttat ctattatacc agtggttgcc atgaatatga atgaagacga
      601 gatttatcgc catatccggc aggcactttc cagtgcgcct cgtaaccagt acaccgtcga
      661 gctgcacctg caaatgatta aatatgccga caaactggaa cacattacgg caaaagcctt
      721 ttgcgaaggc actggtctga gccagagcct cggtaccgaa ttcagcaaaa tgcgtaactt
      781 aacccgtcgg ttaaaggccg ccggtctgaa taccgatctg ctgtaaccga cttacggcac
      841 attatcgcgc aaaacgagat atttaatggc tatcaaaaaa accgaactct attcttccct
      901 gtgggcaagc tgcgacgaac tgcgtggcgg gatggacgcc agccagtaca aagactatgt
      961 actgaccctg ctgtttatga agtacgtttc cgataagtat aaaggcgatc cttacgggat
     1021 gatcgtgata cccaaaggag ccagctttga cgatatggtt gcactgaaaa atgacaaaga
     1081 gatcggcgac aaaatcaaca aaatcatcca caagctggcg gaagagaacg gcctgaaggg
     1141 cgtcatcgac gaagccgact ttaacgacga agataagctc ggcaaaggca aggaaatgat
     1201 tgaccgcctg agcaagcttg tcggtatctt tgaagggtta aatctctcca gcaaccgggc
     1261 cgaaggcgac gatctgctgg gcgatgccta tgaatatctg atgcgtcact tcgcaaccga
     1321 atccggtaag agtaaaggac agttttacac tccagcggag gtgtcacgta ttctggcgaa
     1381 ggtgattggt attactcccg atactccgca agatgcaacg gtttatgacc caacctgtgg
     1441 ctctggttcg ctgctgttga aagtgaatga tgaagcccgg cgcggactgt cgattttcgg
     1501 ccaggagatg gataacgcta ccagtgcgct ggcgcggatg aacatgattc tgcataacaa
     1561 tgccaccgcg aaaatctggc agggcaatac gctgagcgat ccgcagtgga aagaggcgaa
     1621 cggcaaactg aaagccttcg attttgccgt ggcgaacccg cctttttcca ataaaaactg
     1681 gaccaacggg ctgacgccga aaaaagatcc gtttgaacgc ttcggctggg gtattccgcc
     1741 ggaaaaaaac ggcgattatg cttttttgtt gcatatcatt aaaagcctga aaagtaccgg
     1801 caaaggcgcg gtgatcctgc cccacggcgt actgtttcgc ggtaacgccg aagcgaatat
     1861 ccgtgaaaac ctaatcaagc agggctatat caaaggcgtt attggtttgc cagccaacct
     1921 gttttacggc accggcatcc cggcgtgcat cattgtgatt gataaagagc acgcccacag
     1981 ccgcaaaggc atttttatga tcgacgccag ccggggcttt atcaaagatg gcaacaagaa
     2041 ccgcctgcgt agccgagata ttcaccgcat tgtggacgtg ttcaaccatc agcgaacggt
     2101 acctggctac agccggatgg tgccctccag cgagatcgcc ggaaatgatt acaacctgaa
     2161 tattcctcgc tatatcgaga gcggcgagcc ggaagattta cacgatctga ccgcgcactt
     2221 acagggcggt attccggcac gcgatgtgga tgctttgcaa gattactggc gggtattccc
     2281 tgccctgcgc aacgtgctgt ttgctgacga ccgtcccggt tactgtcggg cgcaggtgaa
     2341 cgcgcaacag gtgaaaccga ccattctggc gcatcaggag tttaaggatt ttgccacccg
     2401 cagcctgcta ccgtttaaag cgtgggtgaa agaggcaagt ctggaagaga ttcgcaaagg
     2461 tgataaaccc aaagcgctaa tccacgatat cagcgaaatg ctattggcgc agtacgccaa
     2521 cagtgagttg ctgaacaaat actgcgtgta ccagatcctg atggattact ggactgatgt
     2581 catgcaagac gatgtgtatg cgattatgca ggatggctgg caagccgccg cgcaaatccg
     2641 cgagcttcag ccggtgaaag gcaaagatgg caaaaacatc tggaaagaaa cgcacgattt
     2701 cgagtttacc aaacggcgct acaaggccga tgtgctgccc cgttcgctgg tggaaattcg
     2761 ctgtttcccg gaacagctgg atgcgctgaa ccgcgcgcag gagcgcagtg aagaagccag
     2821 tcgtcagcta gtggaattca tcgaggagca ggcaggggaa gaggatctgc tggctgaggc
     2881 gaaaaacgat aaagataagg tgacgcaaaa gctggtgaat gcccgtctgg cacagttgaa
     2941 gaaaacccct gccgacccgg atgagctggc agtgctgaac cgctgtctgg cgctgattaa
     3001 ggccgaggcc agtgcgaaaa aagcgctgaa agccgcacag gaagcgttgg atcgagcggt
     3061 atttaaacat tatcccacgc tggatgaagc agcgattaaa acgctggtag tgcaggataa
     3121 gtggctggca acgttgcagg cagggatcaa agcggagata gagcgtatta cccagcaact
     3181 ggcgtcgcgc gtaaaggagc tggaagagcg ttatgctgaa ccgttgccag cacttgaagc
     3241 cgccgttgag gcgttgagcg agaaagtggc ggggcatttg cgggcgatgg ggctggagtg
     3301 gtgatggtgg atttaatggt gaatgagcaa cggtcgggta cggcggggaa agatgtgcct
     3361 gcggggtata agctgactga ggtgggggtg attcctgagg attgggtagt aatctctatt
     3421 gatgactgta caaccaaagt tggtagtgga aaaacaccaa ctggtggagc atctgtttat
     3481 attgatagcg gacgcccttt tgtccgtagc caaaatgttg attggggaag attagatctc
     3541 gccgatgtcg tacatattac agatgaaacc cataacacat tctctggttc tgaaattcag
     3601 caagatgatg ttttactcaa cataacggga gcatctatcg gccgatgcgc taaagctgat
     3661 tttagggtta taggtggtaa tgttaaccaa catgtttgca tcatcagaac agaggcaaaa
     3721 aaactctccc catccttact ggttgaatta atcaattcaa attatggaca aaaccagatt
     3781 gatagttatc aagccggagg aaacagagaa ggactgaatt tttctcaagt taaaaatctt
     3841 aagtttgcta ttacaccaaa ctcctttgaa caaacggaaa ttgtcagaac attaaatgat
     3901 attaatgcat tcactctcgc tctggaaaac ttaattgcta aaaaacaggc cataaaagcc
     3961 gctgctatgc aacaactact taccggtaaa acccgtttgc cacagtttgc gttgcgcaaa
     4021 gatggctctg ctaagggata taaaaagagc gagttggggg cgattcctga agattgggta
     4081 gtcacaagca ttggacaatt tactgactgc tgtgctggtg gaactccgag tactaaaatt
     4141 tcagcatatt ggggaggaac tcatccttgg atgagttccg gcgaattaca cctaaaacaa
     4201 gtgtatgctg tagccgatta tataaccgac gaagggcttg ttaactccag tacaaagtat
     4261 gtgcctaaaa attctgtatt agtcggattg gcaggacaag gaaagactcg aggaactgta
     4321 gcaattaatc gcattgaatt atgtactaat cagtctattg ctgcgatttt ccccagcaaa
     4381 catcattcca cagaattcct gttctataac ctcgacagtc gttatgagga actgagaagt
     4441 ttatctactg gtgatggggg gcgtggtggg ttgaatctta ctattatcag gaaattacat
     4501 cttgcatttc cgccaaaaga agaacaaacc gccatcgcca ccatcctctc cgatatggac
     4561 aaggaaattc agaccctgca acagcgtctg gacaaaaccc gtcagcttaa acaggggatg
     4621 atgcaggagt tgttaaccgg gaaaacccgc ttaatataaa agggaaacct atggcctatg
     4681 actacagcgc cagcctcaac ccgcaaaaag ccctgatctg gcgtatcgtc catcgggaca
     4741 atatcccgtg gattctggat aacgggctgc actgcggcaa cagtctggtg caggcggaaa
     4801 actggatcaa tatcggcaac ccggagctga tcggcaagcg tgccgggcat ccggtacctg
     4861 tcggaacggg cgggacactg catgactatg tgccgtttta ttttaccccc ttctcgccaa
     4921 tgctgatgaa catccacagc gggcgcggcg gcattaagca acgccccaat gaggagatcg
     4981 ttattctggt cagcaacttg cggaacgtgg cagcacacga cgtgcccttc gtgtttaccg
     5041 acagccatgc ttactataac tggaccaact actacaccag tctgaatagt ctggaccaga
     5101 tcgactggcc cattttgcag gcacgggact ttcgccgcga tccagacgat ccggctaaat
     5161 ttgaacgcta tcaggcagag gcgctaatct ggcaacactg cccgatctct ctgctggatg
     5221 ggataatttg ttacagcgaa gaggtcaggt tacaattaga gcaatggctg tttcagcgta
     5281 atctaacaat gtccgttcac acacgggctg gatggtactt ctcatgatca catatacaca
     5341 aggcaattta ctggacgcac cggtggaagc tctggtgaat acggttaata ccgtcggtgt
     5401 catggggaaa ggcatcgcgt tgatgtttaa agagcgcttc cccgaaaata tgaaagtcta
     5461 tgccctcgct tgcaaacaaa agcaggtgat caccggcaaa atgttcatta ccgaaaccgg
     5521 ggaactgatg ggaccgcgct ggattgttaa ttttccgacc aaacagcact ggcgcgctga
     5581 ttcccgcatg gagtggattg aagatggctt gcaggactta cgccgctttt tgattgaaga
     5641 gaacgtgcag tccatcgcca ttccaccact gggcgcaggc aacggtggtt tgaactggcc
     5701 ggacgttcgg gcgcagattg aatctgccct tggcgacctt caggacgttg atatcctgat
     5761 ttaccaaccg actgaaaaat accagaacgt tgccaaaagc actggcgtaa aaaagctcac
     5821 tcccgccaga gccgccatcg ctgaactggt gcgtcgctac tgggtattgg ggatggagtg
     5881 cagcctactg gaaatccaga aactggcctg gctgttacaa cgcgccattg agcagcatca
     5941 acaggacgat atcctcaaac tgcggtttga agctcactat tatggcccct atgcgcccaa
     6001 tctgaaccat ctgcttaatg cgctggacgg cacctatcta aaagccgaga aacgcatccc
     6061 ggacagccaa ccgctggatg tgatctggtt taacgatcaa aaaaaagaac acgttaatgc
     6121 gtatctgaac aacgaagcca gagagtggct acctgcgctg gagcaggtca gccagttaat
     6181 tgacggcttt gaatcgccgt ttggtctgga gttgctggca accgtagact ggttgctgag
     6241 ccgtggcgaa tgtcaaccaa cgctggattc cgttaaggaa ggactgcacc agtggcctgc
     6301 gggcaaacgc tgggccagcc gtaagttgag gctgtttgat aataataatc tgcaatttgc
     6361 cattaaccgc gtaatggagt tccattgctg agggtttaag gagcgccttt tgagtaccgt
     6421 gggccagcgc gaacgcgcca cacagcagcg tctgattcag ttttttgttg aagatttagg
     6481 ataccgccat ctgagtcacc gcaccgggta caccgggaac agcaacatcg acactaatat
     6541 actgagcgcg tggctaaaac ggcgcggcgt taatgatacg ttgatcaccc gggcgttgcg
     6601 gcaactggat tccgccgccg ctctgggcga aggcaaaaaa ctctattacg ccaataaaga
     6661 ggtctatcgc ctgctgcgtt acggcgtgaa agacaaagaa ggtgcaggcg agcagaacca
     6721 aaccgtgtgg ctgattgact gggccaaccc acaagccaat gatttcgcca tcgccgatga
     6781 ggtgacggtc aaaggcgagt actgcaagcg cccggatctg gtgctgtacg ttaacggcat
     6841 tgcgctgggc gtgattgaac ttaagcgttc gtcagtgcac gtcagcgaag gtatccggca
     6901 gaatctcgat aatcaaaaga aaacctttat ccgcgatttc ttcaccacta tgcagttagt
     6961 gatggcaggc aacgatacgc agggcctgcg ctacggcacc atcgaaaccc cggaaaaata
     7021 ctatctggag tggaaagaag ataatccaag cgactataca cataaactcg actttcattt
     7081 aagtcgtctg tgtagcaaat ccaggctgtt ggatatcgtc cataattaca ttgcctttga
     7141 tgtcggggtg aaaaaactct gccgccataa tcagttcttc ggcgtcgagg cggctaagca
     7201 acatattgcc cgtcgtgaag gcggtattat ctggcacaca cagggatcgg gtaaaagcct
     7261 gacgatggtc tggctggcga agtggatccg tgaaaatgtg ccaaactccc gcgtgttgat
     7321 tgtgactgac cgcaccgaac tggatgaaca gatcgaaagc gtgtttatgg gcgtgaatga
     7381 agatatttat cgcacctcca gcggcaacga tcttatcgca acgctcaatc atcctaaccc
     7441 gtggcttatt tgctcgctgg tgcataaatt tggttgtcgc agcgaagctg aagacaatgc
     7501 ggctaccgac gcctttatca ccgaattaca gcagtccctg acgaaaacat tccgcgccaa
     7561 aggcgatctg tttgtttttg tggacgagtg ccaccgcaca caatccggca agctgcataa
     7621 cgccatgact gccattctgc cggacgccct gtttatcggt tttaccggca caccgctgat
     7681 gaagaaagat aagaagaagt cggttgaggt gttcggccct tatatccata cctataaatt
     7741 tgatgaagcg gtggccgacg gtgtggtgct ggatctgcgc tacgaggcgc gcgatattga
     7801 ccagtatctg acatcagaga aaaaggtcga tgactggttt gaagccaaga ctcgcggttt
     7861 gtcgaattta gccaggaccc agcttaagca aaagtgggga tcaatgcaga agctgctctc
     7921 cagcaagtcg cggctggagc agatcgttaa cgacattttg ctggatatgg atacccgtcc
     7981 acgcctgatg gacgggcgcg gcaacgccat gctggtatgc agcagtatct atcaagcctg
     8041 caaggtttac gagatgttca gccagaccga gctggcagga aaagtagcga ttgtgaccag
     8101 ctatcgcccc gatgccgcca gcatcaaagg cgaagaaacg ggcgaagggt taacggagaa
     8161 actctctaaa cacgccacct atcgtaaaat gttggctgac tattttgaac aaagtgaaga
     8221 gaaggccgcc gcacgcatta ccgagttcga gcgggaagtg aaacaacgtt ttattgaaga
     8281 gccggggcaa atgcgcctgc ttatcgtggt ggacaagctg ttgactggat ttgacgcgcc
     8341 gtccgccacc tatctctaca ttgataaaac catgaccgac catacgctgt ttcaggcgat
     8401 ttgtcgggtg aaccggctgg acggtgagga taaagagtat ggctatatta tcgattataa
     8461 agatctgttc cgttcgctgg ataaagcgat taccgattac accacgggcg cgtttgatga
     8521 ttacgatcgg gacgatgtgg ccgggttgct gaaagacagg ctggagcagg cgcaactgga
     8581 tctcgacagc gcgctggaga tggttcgcag cttatgtgag cccgtcaaaa ccccgcgcag
     8641 cctgcaggac tatcagcact atttttgcgg taaatcagga gagaatcaga cttcgttaaa
     8701 cgaaaaagag gcgctgcgcc tgacatttta ccagtgcgtc gcccgtctgc tacgcgctta
     8761 tgccagcatc gccaatgaaa tgccggaagc aggctacact gccgaagagg tagagagcat
     8821 tcgcgccgaa gtcgctgaat tcgaaaaact gcgctatgag attaagctga ccagcggcga
     8881 tcttctggat atgaagcgct ttgaacccgc tatgcgtcat ttactggata tgtatgttcg
     8941 cgccgatggt agcgaagtgc tgatggattt tgaagagtta agtctgattg aactgattgt
     9001 cgagaaaggc ttggcggcga ccgtcgccct gccggatgat atccgtaata atcaggatgc
     9061 gatggcggaa accatcgaaa acaacgtgcg caaaaccatc gtggatgaaa acccggttaa
     9121 cccaaaatac tatggacgca tgtcgcagct gctggatgaa ctgattaccc ttcgccgaca
     9181 aaatgcgctc aattatcagc agtatctgga acgtatccgc gatttgtgta aacaggtcat
     9241 ccgcccggag caaaccgccg gagcgtgcta ccctgccacc atggatacac aagcaaagcg
     9301 cgccttctat gacaacttcg gccacgacga ggtactggcg acccgcatag atacgacgat
     9361 tcgctatacc aaaagggcag agtggattgg cgatcgtttt aaagaacggg aaattgccaa
     9421 cgcattacgt gaagagaccg ccagctataa tattgatatt gatgaggtga tagcgcttgc
     9481 ccgtcagcag aaggaatatc actgatgcca atcatgcaaa taggctcgtt acacttgctg
     9541 gtcaaccgta aggcgattaa gaatctgcat atcagcgtgc tgccgcctga tggcaaagtc
     9601 cgcgtttcag cgccggaaca tatgacggag acggcgattc gtatggcggt tgcttcacgc
     9661 tttcgctgga tcagaaaaca gcagcaggat ttcgccagac agcctcgtca atcagagagg
     9721 gaaatggtca gcggagagtg ccactatctt tggggccgaa gatatcgcct acttgtgatt
     9781 gagcgtcacg ggcgtcacga agtaaaagtt gcgggaaata ataagctcca gctttatgtt
     9841 caaccgggaa cccgtgatga aaataaggca ttggcgttaa gtgaatttta tcgacatgaa
     9901 ctaaaacagc aggtggctaa gcttctacct gaatggcaag accgaatcgg tgttccccta
     9961 acattctggg gcatcaaaaa gatgaaaacc ttttggggca cctgtaacac tacaacaaaa
    10021 cgaatctggc tcaatctgga gctaattaag aagccgccgg agtgtctgga gtatattctg
    10081 gtgcatgaac tggttcatct tctggaacgt caccataatg ctcgcttccg gttgcatatg
    10141 gatcggttta ttcctgactg gcgtgagcgt cgggatttgt taaataatat gccgttagcg
    10201 tttgaggcat gggagtattg attgagcctg taactgtttt ttgtccataa attaaccttc
    10261 atttgatgct ggatagaaca tatcaaaatc agacaattac acaaatctat gtacaggctc
    10321 aactcctgcc atggaacgta gtactcacca ataagtaagc atcaatacgg cgctccgtta
    10381 acgcttacta gcatggttat ctccttaaaa aattgaggcg acaactatgc tgacacaggg
    10441 ggttactcac cgatctcctt cacggtaata cgcagttctt cagaacccgc ctccgatacc
    10501 agcctggcgg tcgccccggc gcaacagaat aaatcaacca tcgtcagact gcgatcattc
    10561 gtatgcatcg ctgatgatgc gcccgaaaaa cgtgtaaagc tgtgacagat gacgcgcaaa
    10621 aaaatcccgg cataatggcc gggatttgcg agctttccca ctatttcttg attcctaaac
    10681 ggaacatatc agttgggaat aaaggttgta ttatcacttc atcattaaaa atgaataatt
    10741 tgggcgataa agctgttacg tcatagatat tttcagcgat taatcttaga tttgacctaa
    10801 aaactggaat acttgcatca tctgcaaaga caaacatgtc atcgtttttt acagagtttg
    10861 cacacaatag ctcatcaaaa ctctcataat cagaatgcca gatctctgac caaataacac
    10921 ttcctttcgc gagagggtac ttatttaaaa tgcccagagc ctcctgctga gccagcccct
    10981 ctactatatt aaaatcagca ctcagtgctt cccgacactc atcaaataaa gtcatttgct
    11041 ctacctgttt agctgtttca atattgtttc aaaagacttt tcattgcctt caaaaggtat
    11101 gacttgcttc accgcttttc cagggccttt accctgcgag taatcccata tattgatgtg
    11161 tgtgcctttt tcaggatcat aatcaacacg ccaaccaact tttccgtcgc tggattgccg
    11221 ccctatgact ttcccattac cagcgctgac ttccaaacgt ccgataactg gcttagagtc
    11281 cgctcctaaa ttacctacaa gatccaatgc cttgtttcga gcagtttccc agtttttctc
    11341 tggcttgagg atttgttcaa ttttaggctg ggaattccct ttaaccttac taattccccc
    11401 aaatgctgac gccgcagcgc tcgcgactat tcccgcctga acttcatcca aaccgtattc
    11461 ttcttggagt gcttccgaga ctttgttggt cgcgacaacc ccttcagcct gcatttgctg
    11521 cgcaaaagtc gccgtcagat cgtagaccat tttgatcggg atatcttcac ccagagcacg
    11581 atctattgcc tgcttcaccg ccagactgtc agtcagtaca tcaccgtatt tctgctggca
    11641 ggttgccggg ctggctgcac agtcactgat cagttgcttt tgctgtgccg tactcaactc
    11701 ttcgtacttt ttgataatac cgccgcagtc accgcctttc gcctgacagg acttcatttc
    11761 agcggaccaa gcatctatct gcttactgct cagatagtta ttatcaacag cattcttccc
    11821 gctttgtgct cccactgctg ctgatgcggt gctgtttccg gtaagacctc cggccagccc
    11881 tgctgacacg gttgccagcg tacttatcgt ttgcttctgc tcttcactga ggtctgactg
    11941 tttcacgccc ggatacagca tgccggcaat cgctctggct gccagctctc ccgttgctgc
    12001 gcctgctgca ccggcagcaa cattattacc ctgcattgcc gccactgcgc cgcccaaaat
    12061 ggcgtgagca atggcgttta ctgccgggtc tttttctgtt gatttcagca ggtgcgccag
    12121 ttccggagcc gaagttccgg ccagagcacc cgctaaatta ccccccgcca gcccctgaag
    12181 cgcggccgtc gcggcctgga taccccgctg aatatcaccg ccggtgccat acttcgccat
    12241 ttcattcctg taagcctgcg tgtcacgcag tttcgcaaga tattcctgac gttgtttctc
    12301 tgttgcatta gcaggaagtg tttcgccggt tgcttccttc gcggccttca acgcattcag
    12361 ttctccctgt gtacgcgcaa tatccgccac ctgactgcct atgtcactga taagccccac
    12421 tgtctgcaga cgcctctgct ctttctcctt gtcaaatatc gggctgatac tgtcattagc
    12481 gtgcacaggg tcacggctca ggttcgccag attctgcttc tgattgtccc tgtcccggat
    12541 ggtgatagtg ccttctgcca ctgcggcctg agtcgttcct tccgcatgtc cgctgtgacc
    12601 tccggcggat atcatgccac ccggcatgtt accctgaaat ttatccccga agctgccacc
    12661 accgctcaga ctgattccac tgtgactgac tttataatcc gcttcgttgt gaaggtcact
    12721 gaaccccagc gtaccggtat ccaggtggtt tttatccggt gtggcagtag aggcaatcac
    12781 cgcaccatcc agttgggtat gtctgcccac tgtaatgtcg aagccgccgt caccggcaaa
    12841 cattccggtt tgttcagcaa cggagtcaaa gcggctcttc atcttatccc gggaggcagc
    12901 gatgtaacct gagccggtca tggagccaaa ggtaaaactg ccgctggcag ccacgctggt
    12961 ctgtttactg tcgtacttac tggtgtcctg ctggctgctt atcagcaggt cgtggcccac
    13021 atcggcgata atcctgttgc cgttgacctg ggcaccattc agtaccgtat cccgaccact
    13081 gttgatggtg acggttttac cgctgtctgt tgtggtttca gtccactcag taccgttacc
    13141 tttctcgctg ccttttgccg cattaacgct ggcaaagacg ctgataccgg caccgttacc
    13201 accggcaccg atactgacac ccacgccacc gccactgctg ctgttcctgc ccgttgtttt
    13261 ttgtgtgttt gcagcgccac tcaacagaac atcattcgcg gcatccaggc ttgtgttacc
    13321 accggcctta agctggcttc ctgcaatcac aatatctccg cggttatcgc ccctgttttt
    13381 gccggttgcg acaacagaca gattattccc ggcattcagc gtactgccgg atactgtgtc
    13441 actttcagaa tgttgttgtg atttcgattt ctgggtggtg agcgacaggc tgactcccgt
    13501 cgcattcggg tcaccggttg cggtggccat tgccgcagcc tgtccggcct gcacaccaga
    13561 cagcgctgtc tttgtggcct gcagggcttt cagacggctg tcactgctct ccttcgtctc
    13621 ctgtgcactg gtgaccgcat tattgatggc actgcccacc gtgccggaaa gggcaaccgt
    13681 cagcccgctt ttcttctgct caaatttttc gtccacagta cgacggtcat gccccgggtc
    13741 aaccaccaca ctgtcaccgg taatgctgat atcccggttc gcaatcacat ccgaaccgct
    13801 gatatgtacc tgtttgcccg cggtaatact gacattaccg gcagtggagc cgatggtact
    13861 ggcactctga ctctgcgttg tcccggcctc gcggcggtcg tgcgttgtct tactgctgcc
    13921 agtggtgaag ccgataccgc cggtacccgt cagaccggat ttcttcgttt ccttaaagcg
    13981 ccaggacgta tctgtactgg tggcagcaag aacatcaaca tggttacccg ccgccagtga
    14041 cacatcccgg tcagccacca catccgaacc ctgtaccgtc aggttattac cggcgttaac
    14101 ggtcacgcgg tttcccgaca gcagggagcc tttctcccgg gtgacgctgt cttccgtgat
    14161 ggtccgggtg gtctgatggc tgaggagacg gttacgggtt tcccctgatt cactgtagtg
    14221 ataatcactt tccgtggcag tacccagtac gatgtcatga ccggcattca cactgatatc
    14281 gccctttgcc gttacagcgg tcgcaactga ggtgatatcc cgtccggcag taaccgtgat
    14341 gtcaccgcca ctggtcatgt ctgttccctg ctgccggacc gaatcatgaa ccgtcttcat
    14401 gctgaaagtg ctgtcgtggt cacctgtgcc tgtggactcc gcagccatgt tcacatcccg
    14461 tccggcgcgg atagccatac tgttttcagc cgtaataccg gcagccaggc tgttcacatc
    14521 acgaccggcg gaaagcatca gactgttccc ggcagtgacc gtgggggcca cggcatgagt
    14581 ttcataccac ccggacccgc catagcgacg acgttcgccg gcctgtatcg cgttcatgtt
    14641 caggtcatta ccggcggcca gtaacacgct gtcaccggca gaaacggcgg caccggtgat
    14701 actgatatct ccggctgcgc ccataaacag tgagtccgtt gccctgacag cagccagcgg
    14761 accggtatcc gttccgctga aatggttata tccgttgttc atcgcccact gttctgtccg
    14821 ggtgaggtta ctgatgtttc cggtggcgct ttccagggat acggttttac cgctgatgac
    14881 tgagctgata ttgccgatat ccccgatggc cttcaggttc agtgcaccac cggcgtttat
    14941 cagtccggca ttcaggttgt cgatactgcc tgtgctgtcg agcaggaggt cattctgtgc
    15001 gttgatgctg ctgccgctgc tggtgatatt gccacccgca agctgcacgt tgttcccgct
    15061 gataacgctg ccattatgaa gggtgatatc ttccggagac agatacagtt tcggaaccat
    15121 gactgtctgt ccgttgatgg tgacggactc ccaccacagt atgcttctgg tcagttgtgc
    15181 aacctgcccg gcggtcagcg acactccaaa gctcagtccg agcgctttct gttgcgctgc
    15241 cgcgttgtcc atcaggtatt tcatctgcgc caggtcagag cctgtgccgt taatataacg
    15301 tgagcccgtc tggttcagta tgacgttact gacataccgg gtatcaaaag ccgcatcccc
    15361 cagaaagcgg taatcttttt ccggtttcag gccaagatgg tcaagaatgt acgacgagcc
    15421 cagaaactgt ttttcatcgg tatatgccgg gtctgtttct cgtggtgcct gtccgggttg
    15481 catcctgagc aggtcataca gtccggcaaa cagactgctg tccacctttc cgagaccatc
    15541 cagtttcggg ttcaccgtaa tcagatacgg actgtccggg tccgtggacg ggacaaaata
    15601 accattgtta ccggatggca ggggatagtt gcttacactt gccccggaca gcgacagcgc
    15661 acccgggata tccttcagcg tatccggcag cgcgtcattc catacagggc cggttatccc
    15721 ggtaccgctt tcattccgca gggcttcacc gctcaccccc tgactgaccg tcggtggtgt
    15781 cagggagttc agcgaaggca ccgttatcgt gttacctgca ccaccggccc ggggcatgac
    15841 agtggtatta ccggtgtcag tggtaaagct ggcagacaca tcaccggcag cgctgataac
    15901 cgcccggtac tgacctgccg gagaggtgcc actggcttct gtcgcatccg gtgagacctg
    15961 gcgatagacc ctgtaccatg gctcagtaaa ccatgctttg gggagtgaac ctgaggcaag
    16021 ccgccagacc gtttcctttc cggtggtccc ctcctgccag ccctgattgt tcagtgtgtc
    16081 gccactcagg gtcataccgc caccggcgag aataaacgat gcctcattat ccagtcgtcc
    16141 tgcacggata tgcatatcag cgccgccgga tatccgggca gcatgaccgg aggcactgac
    16201 tgtcaccgtt tccgtatcgc ccgtacggat acggtattca tcaggtaccg tggcaccctc
    16261 cccccagtac acggccagat attccacacc atcatgccgg acaacacccc accaccggtt
    16321 attttccgga accggtgaaa tcaccccgtg actgcttcca ggaatgacct catggctggc
    16381 cgaaatggca tcccaggaat tcagcagatg cgccgtattc atggtgatat cgccacgtgt
    16441 ggtttcaata ttaccggaac ggttaatgat ttcgctgttt gccgtcccgc tgctgtccct
    16501 ctgtacccac agactgttgc cggccaggat atcaccatgc tggttatgca gacggtctgt
    16561 aaacagcttc aggttattcc ccgcataaat cagcgcactg ttcagcaggg taccggccac
    16621 attcattgtc agactgcctg ccgtgccggt aaaaccactg atggtgatat cactccggct
    16681 gttcagactc acatcgccac cggcctgaag tgaacccggt gcgttaagga aaagacgctg
    16741 tgcgctgaaa acactgttgc ctttaccggc agtcagcgtt ccattgttgg tgaatgcccc
    16801 tccggcaccg agcaccatgg catcaccctg catcacaccg ccgttggtga cggcgttctg
    16861 cgacgtgacg gaaagggttt tccctgcggc cagggtaccg taattcgtga gtgcagcaat
    16921 cagtttcagt gtgacatcac cggtggccac cacctgaccc tgaccactga agtcctgagc
    16981 gtcaagcagc aggttgcctg cactgtacag ccgccctgta ccattttgca gcagtgaact
    17041 gcccttgacg ccaagcccgg aagttcccag cagggtaccg ctgttgctga atgtgtggta
    17101 attcaccagc aggtccgcac cctgaagcgt accggtatta ttcagcgtgg ttcctttaac
    17161 gtcggcactg ccggtggcaa gtacgcgtcc gccgttgaca gtattcacca catccagcag
    17221 cagggtggca gcctgtacca gtccgctgcc ggtgttcgcc agcacctgcg ccgtcagcgt
    17281 gaggttactg ccggagagga ttttgccgtc gttctgcaga cggtcagtgg cgttcaggga
    17341 aaccccgcca ccaccctgta tcgcgccctg attactcagg gtcgcagtac tgacattcag
    17401 tgcattccgg ctcatcagaa caccaccgga acggttgttc acgccaccgg aggcggccag
    17461 cgtcagtgtt tcgccctgca gatgcccgtc gtttgtgagt tgtcctgccg tgatggtggt
    17521 ggtatttccc tgtaattgcc cgtcgtttgt gacactgtct gccttcagcg tcagcacacc
    17581 tgcactgagc agttttccgc tcgcgtgatt gttcagcgtc tgattcaccg tgagcgtgag
    17641 agcatccaca ccggtgatgt cacccgcact ggtcagtgag ttcgccttca gggtcagatt
    17701 ttttgcaacc cattgtccgc tgttgcttaa attcagtgca ctgagcgcca tttcaccgtt
    17761 cgaggtgact ttgctgcccg ctgtgctgac gagctcaccc gtcagacgtg cagtcaggct
    17821 gtcagccgcc tggatcgccc cgctgtttgc cagactgtct gcggtgatca gcacccgttt
    17881 gccctgccag tgtccggaac tggtaatact gcctgcggtg attgtcagat caccgctggt
    17941 cagcaatgaa cctccgttat tcatcagcgc aggttgaggg gatgccatac gggcggcaag
    18001 cgtcagcgcg gcgatcccgg tgagcgtgcc actgttggtg acactgttct gacgaatcgt
    18061 gacatggtta ccctggacag tgccgctgtt atccagtgag tttccatcaa gggagagcgt
    18121 gccggccgaa agcagactgc cccggttgtc cgtggtggct gcgttcagcg tggtgtcacc
    18181 ctggctcatg atatcgccgg tactggtcaa ctgaccggtt gccgaagcag taaggttacc
    18241 ggttgccagc acggaaccac tgttcgccca tctgtccgcc tgcacggtga gattctgtcc
    18301 ctgcgtggtt cctgcggtat tcagtgtttt accccggagg gagaggtcgc ccgccgtcag
    18361 ccagcgtccg ttacggccct gtgagagggt gtcgcctgca agcgccagtg caccggcgcc
    18421 ctgcaacaga ccatcgccat ccagcgtggt cgccctgacg ttcagcgtgt cagcgatgat
    18481 ttttcccgga ttgctgaggg aggcagtgtt taacgttaaa ccattatcac cggtgataag
    18541 cccgctgttg cggatgtccg gtatatccag cgtcaggtcc gcagcactgt acagcgtacc
    18601 gttctgctga ttatcaagcc tctgtgtgtt aacggtgagt gaggcttccc cctgcaacag
    18661 accgctgttg gtcagggtct gtgactgtgt attcagggcg gaagcgacaa gtacgccgct
    18721 gctggtcagc tcaggcgcac tgaggctgag cgacggggcg ctgcttttcc cgctgtgggt
    18781 gagcttgtca ctggcgttca ccaccatggt ctgttgtgct gcctgcgtac ctgcaagacg
    18841 tgcatctctg gcgttgatgc tgagattttt accgctctga agctgtgcgc ccgctgcagt
    18901 actcagtttg tctgcctgaa cccggagggt gtcaccggca ctggtttccc cgtccagcgc
    18961 cactgttgtc acattcagcg tcatcgcagc atcgctgtgg gtgaccgatt ttttaccgga
    19021 actcagcgcc tgcgcactga ccgtcagccc tttgccgccg gacagcacac cgttctgtgt
    19081 cacatcctgc gccttcagca ccagtacatc atcgctcacc agcgaacctg tactggtcag
    19141 tttcccgctg gccgtgatat ccactttgcc cttcgcgcca gtgcgtccac tctggttaaa
    19201 gtcgcgggta ttcacggtca ggggaccgcc actgagcagg gagcctctgt tgctgagcgt
    19261 tgtactgccg agcgtcaggg aagccccctg aacagcacca ctgttattca gggtgccggc
    19321 atcgagtccc gcatgacctt tcgccagcaa tattccgtcc tgcgtcagcg tggtggcgct
    19381 ggccgtgaga ttctgcccgg cggttatctg tccctgtgtt gtcaacgtgt cactggcgac
    19441 agtcacgata tcgcgggccg cgttaatctg gctggcggta tcctgtgtga tgtttttcgc
    19501 ggcaagcgtt acatcccggc cggcagtcag tttttcattc tgttgagtga ttctgccgcc
    19561 ggcggtcagg ctgaggtcct tgtcgctgtt aagcgttcca ttgctgagaa cgatattgct
    19621 ccggctgctg acgctcaggt tacctcccgc tttatggtcg ccggttaagg tgacgccctg
    19681 accttttgca gtgacggccc ccgtggcgag actgttgttg acagtcagtc tgccgctggc
    19741 atccagggtg atatcgccat cgcgggcata aaggctacca agattaaccc cgacaccact
    19801 ttcagtggag gtcagatgaa tacgcctggc gtacattcca ccgagcgcgc cggtatcaac
    19861 ggcaactttc ggcacatcac cttcgccctt cagggcactg acgcgaccat ctgcagttac
    19921 acggttagcg cctgcagtga cagttaaatc cttcgcatga agcgcggcat tcacttccgt
    19981 tgcacgggca ataatggata cggcatcgct ccgggtgccg tccaggcccg cgccattgat
    20041 ggtgatactg ccttcagtca cctccagcgc ctgcaggctg ccgtcggcat tcatcacagg
    20101 tctgcccgtg gtgagcgtcg cgtgcggcgt gttgataaag ccacagccgt cacaggtgat
    20161 accatacggg ttggcgacca tcacattcgc cgctttgccg gccacttccg tatagccctg
    20221 cagcagcgaa cggttaccgc cggtcacttc gttgatgata cccttcgctt ccccacccgc
    20281 tttcaggttc gggttattct gtatcagtcc accaagctgc gtcggattaa gcttaccggt
    20341 ggcattattg agaatcagcc cctccttccc gacgttgtaa tccgtaaacc ggttatgcga
    20401 aatcccggcc ccgttcggcg tggcaatgtt cacgaccggc acaccatttg ccgctttatc
    20461 cattccggtc ccgttttgtg gggtgatgac ggcccccaca gccggtaaca acggctgccc
    20521 ggcgataatc gtactgataa ggtaactcag caggcggtaa gtgaagcgaa cgggaggctg
    20581 atgcatggta ataatccctt aaaacgcgac ggcaacgcgc cagtaaaccg tgagatggtc
    20641 aggggcaagc cagtccgggt aaaccagagg cagtccggcg atgaacgaac cggaaacatg
    20701 accactggtg gtgctgagcc cggcagcagc accccacagc gtgccggacg agtacgggtc
    20761 atcgctgtca gagtgcagcc agccgccgtc cagtgcagtc actgcacgga cagttcccac
    20821 atatggcagg gagaacagag accaggacag ctcattccgc agataaccgc cgttattacc
    20881 ggagatatac tgctccttaa agccacgcac tgaactctca cccccgaggc tcagttgttc
    20941 cacaccatga agacggtccg gtgaccactg ggcataagcg ctggtcagcc accacacact
    21001 gtccgtgacg gggcgctgaa aactggcgct taatgaccat ttccggaact gatttacggg
    21061 caggtctccc cttttcccgt ggtcgctttc tgcgccgaac cagggcatcc cccgtgtaaa
    21121 taccggattc agtgttccga caccacccag aaacttgtgt gtgtgattca gcccgacaga
    21181 aaatgaagtt agtttacggc tgctgccctg aagcagaaca tcatccagat aattgtgaat
    21241 aatgcggtgc tgcagacctc cggtcagtgc tgtcttcatg tccccgttac ggaacaggac
    21301 atgcgacagt cccagccggt gagtctgcag gtctcccgtg gaacgccacc gccagccccg
    21361 gttatcaatg gtgctgaggt agtcactcca tgaatacgtg taatccacca gggtatagcc
    21421 atacggcaga ctgacaccgg cggcaaaatt cctcgcatca tgtgacaccg aaaagtcact
    21481 gctccgtccc ccgctgacaa accagttgtc agccagcccc agaggattat taaaggaaag
    21541 gacaccattt aactgccccg taccggtatt cttctgtcca ctgttgtcga agcccacgct
    21601 ccctgtgaca ggccattccg gcgatgccgt cagtgtcacc accgaccatc cctcacggtc
    21661 accgggcgat atttcaatct gtaccggctc cgtacgcaga cgattaatct gctccatccc
    21721 ctgctcaata tcccgcaggt tcagaacctt cccctccatt cccgggaaaa ccatcttcag
    21781 ggtgcgggca ggaaggtcag cgccttccgc ccggatttgc tgcagcctgc cttccatgac
    21841 cgttatgtgc agtacgcccc ctgaaaggtc ctgctctgtc agaaaggccc ggctggtgat
    21901 atatccccgg cgtatatagc cgtccgtcac ggcatccgtg accgcggtca gccccgtgat
    21961 attcagacac tgattcaccc acggtgccac cagtctgtcg gtttctgcag acgtcagtcg
    22021 ggtggcccca ctgacaacaa tgcgtgacac cgtaaagcag gggccttcag taccggcaga
    22081 catttccgga gacggcgtga tggtcagcgg cgcactgcgc tccagcgcat cacgctgacg
    22141 ctggttttca tccagcaact gctgctgttg ctgctgaatt gcactgcggt cacccggaga
    22201 cagcatggct gccggagacg aaaatgaaag aatgcaaagg caggggagga gggaaaaata
    22261 gcgtaccacc tgagataagg tacggatcct gaatgcgtac tgaccggaaa ccataccagg
    22321 aaacgacgtt ctgttcgcca gtaagttatc ctgccggtgc tgcatattct cctcgtgtcg
    22381 tgggtattca gtgtaaatta atgtaagtgg aatgtcagtg ctgaaattta caccagataa
    22441 aaaacataca gaaacaatcc tgttatgtaa aatccggaaa aaatgtcaga aatgtgatca
    22501 gagttgtgtt ttacggaggg gagaaaggaa aacagtcatc ctttgatggt aaaaaagatg
    22561 tttcctgcag agtaacactc agggatgcaa cacaagttcc agttcaggtg ttgtggattt
    22621 gagtaaaaaa acagtcataa ctctcctgag aacctgaaac tcctcagtgt gttcttataa
    22681 atggaaaatg cgcaataacg atgattccag tcactactga taacgcgttt tgcgtatgcc
    22741 gaactgtgat cgattcaggt gtcattttgc acgtttctca tctttgatgt aacccaccat
    22801 ccctgacctg gcatcgcaat aaaattaagt ctgaataaaa attacgacaa tttcacagtt
    22861 tccgtcgatt gtggcattat caacagcatc cacctccggg gtttacaccg gagcctgaac
    22921 aggtaaacta cctgttcatc ctgtttattg tccctgaatg cttatgtcgc agttcgattt
    22981 taccagtccg gatccctttg cccgagccat ttctcctttt gaggagatgg cggcttatga
    23041 ggcgttatgg tcagaaaaag gcgcgacatt caaaactatt gcggataaat ttcgtgcagc
    23101 accaaccggc tcgcttcctt ctgactttgt ctcagacagc attattgctg cttttaaaaa
    23161 ccggctgaaa gaaattatcg cacgttacga tattgacgat ctgggtatac gtgtccatgg
    23221 tgccggagaa tatccagaaa aactgcggga tgcgcggaat cccatagaac ttctttacta
    23281 tcaggggtgg tgggacctga ttaacactcg ctcggtagct gttgttggtt cccgtagagt
    23341 ctctgaagaa ggagcaaaac gaacccgcaa agtcgtaaaa tttctggttg aggatggttt
    23401 taccatcgtt tcaggactgg ctgaaggcgt ggataccaac gcgcatcaga cggcactggc
    23461 tctgggtggt aaaacgattg cagttatcgg gacaccactt tcacatcatt atcccagaca
    23521 aaacagtaat ctgcagaaaa acattcgcga caaccacctg cttatcagtc aggttccgtt
    23581 tcatcgatac ctggagcagg actatcggag caaccgaata ttttttccgg agcgaaatgt
    23641 cacgatgtct gcactgacag aagcaacgat tattattgaa gcatcagaaa catcaggtac
    23701 tctgactcag gcacgtgcgg cacttgccca gggccgaaaa ttgtttattc tggaatcctg
    23761 ctttcgtaac ccatcaatta catggcctgc ccgttttgaa gcgcaagggg ccattcgggt
    23821 caggaattac gaagacatca ggaggcatct ccgttgacac ttcgtctgac tctgattgat
    23881 gaactgatac gtaaacaaca tcattatctg gaggatggtg atctgtgtta ttgctttggc
    23941 gaatacacag cccgacaagg cgctgcttac agcgaaacta accagttaat catcaatctt
    24001 aaaaaggggt atgaacgtcg ggcattccct gattatcact ataaaaaggt tgctattgag
    24061 tatgttgctc agatgctcat atcaaatatc ggtaatcttg aggaatacac atttgttccc
    24121 gtaccaccat ccaaatgccg tacagatgcc gcttacgatg accgtatgac agaaattctg
    24181 cgaattgcgc aaacctacga accctggctg gattaccggg aactggttgt gcaaaacgta
    24241 tcaacaattg cctctcatac agcagcaaat cggccctcac cagaagcgat tatggcaaac
    24301 taccaactga atgaacaatt actggaggga tgcaggccct ggattgttat ttttgatgac
    24361 gttcttactg ccggtagcca ttttaaagcg atgaaaagcc tgatactaca acatattcct
    24421 gaagcctgta tactgggact ctttgttgcc cgaacaaccc ggggagctca aattatttaa
    24481 aaagggtaaa gtactcctta aaatgaaagc ccttcaccat cagccacaat cctgtacccc
    24541 atcgccttat aaccccgctg gcgtttatcc cacattctga gcagcactgg atacgcggta
    24601 tccacaaaat caatgatcct gacatcgctt ttaccggtat gctctctgtg tagacgtccc
    24661 gcatactgct gtaatgtccc tttccatgac acaggcatgg caagaatcag cgtatccagc
    24721 ggagggtggt caaatccctc accaataagt ctgcctgttg acaacaaaat acggggagaa
    24781 tcaggaggaa gagcattcag cccggaaatc agcatagtac gctttttttt cgataatcga
    24841 ctatggagaa caaagggaga caatttcagt gaattcatca ctgaggctat atcatccaga
    24901 tgatctgtac gttcagtcag taccagaact ttacgcccct gcccaaaagc tttcatggct
    24961 tcttcagcta tcgccaccgt tctgtcatga tccagagcaa tttctctgaa aatatcctgg
    25021 attctcgcat ccgatggtaa atgaccagat gtaaaacggg aacgggtcag cacttccaga
    25081 ttatgtaggc tttccttcgg gcgggccgca gtatggcgaa tggcaccaca gtacataaaa
    25141 ataatgggat gcagaccatc ccgtcggatt ggtgttgccg tcaggccaag cagatatctg
    25201 gcattcgttt ccttcagaat cgcagaaaat gaaaccgcgc caatatgatg gcactcatcc
    25261 acaatgattt gcccataatt cctgaccaga ggttcaactt ctccgtgtct ggatatggac
    25321 tgcaccaccg caatatcaat attgccacag ggtttatgtt tacctccccc gataatacca
    25381 atactgtcac cgacctgcag aaacaccgcg agacgctcct gccactgctt cagcaattca
    25441 gtccggtgta ccagtatcag tgtattcact ttccgtctgg caatcactgc cgctgcggta
    25501 actgtcttac caaaagccgt tggcgcacaa agtacacctg tgtcataacg gagtaatgcc
    25561 gaaacggcct cttcctgctc cgctctgagg tttcccgtaa aaacggcatt acattccgtc
    25621 ccggcaaatc gtttatcgat taattctgca gcaatattgt tgtacctaag gaaagataat
    25681 gcactgtcca gacatccccg gggcaacgca atatgttgcg ggtaattctc cgcacaacct
    25741 ataacacggg gcttattcca gactgacata cgcatggcct gagccttata aaactcagga
    25801 ttcggaaatg ctgccagccg aataagtcgg ttgaccaggg cctgaggtaa ttgcgctttt
    25861 tcgaaataga tctggtttgc cagcgtgatt atcagcggct cagtaacagc aatattcagt
    25921 ctgtttcctg atgatttttt ctcttcccac ggcgtaccca ggtcttcttc gttgataaaa
    25981 ttcacatcca gaggatggat actccctgta gcccgtaata tcgtcggttc aatatcctgc
    26041 acattcatcg ggatcaccga tacaagaaaa gcccactgat caggataagg ctggagattc
    26101 atatcaacaa aaacgcttcc ccccgattca cgcggtcttt tttgcagagg taacgctatg
    26161 agattaccaa atcctccttt tggcatagta tcctgattag gaaataatcg gtcataagac
    26221 cccaatcgca gctgtcgggt ccgactacac gtatagctaa taatagcagt ccccaaacgg
    26281 cgagcttcgc gggccgaaac tcgtgaggca aaaaatatcc agacatgcgc cccctgacgt
    26341 gaacgggata tttccagcgc agcaggtaca cccagctcat cacaggatcg catgaatgcg
    26401 gatgcatcct tctgccattc agcttcatca aaatcaactg ccagaaaata acaggaatca
    26461 tcttccagca acggatacat cccggcagta tgggtaccgg ccagatggtg gtagatgacg
    26521 agatcggata caggaatcaa ctgacggtga gcgcagtccc cacattttat ccggggctta
    26581 ccgcatattc tcgcctgcca ttcgttagcg caggcaggag agtaccctga tttgccgctg
    26641 gttttacttt cccatctaag tgcccataca tcatcacgcc cgcgaaacaa ccgacgaaat
    26701 aatgcaacct tctcattggt ggataataca gaaacgcgct gcacagagat cggcggttta
    26761 cgtcgccatt caatcccatg agcttcaagt aatgagacca gacgggcatt ttctgccctc
    26821 aatgcagcta attcatcgct gtcagattta tgaaccatat tcgttacctg tatccgatcc
    26881 actgaggcac cacggcagaa attcgggtcc cctacactga tgacaaagcg ccccgccctc
    26941 ttctgcctat taactcaagc acctcccaac aacttacaga aacttaattc taatacctaa
    27001 tacagctgta attcagttgt gtcgtggtcg gccccggtta cttttttcag gaagcctgct
    27061 acggcccaga ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta
    27121 acgtaagttg tctaccgcgg agttatcact atagtgaatc acgtgatcga tacgcaccgg
    27181 gtatttgtct ttagccctgc acaggtgaaa ataaccctcc ccttccgtga tccgcctcca
    27241 gatatctccc agttgcctgg atatccgata agatttcttg tgacgttgcc catccaggta
    27301 gccgataaaa tgaatatgaa gtcccttgtt tgacgtatat tccattaccc agtaatatcc
    27361 cgccagcatc gtttgggttt cactaagtaa gcggtgcatt tcaatacaca tactgtgcct
    27421 acaggaatga ccaaaactgg gcgtgtcttt cctgtaggca aaatcaattc tgaaaggtaa
    27481 cagtttagaa aaacgttgaa acagaccttc catgtgttca ttcacgtctt tcagaatcat
    27541 gaagtccatt tcgtaattgg gattagcatt atacatttta tagtccttac tttataaaac
    27601 tgacaggaaa gtggaattac ataaatactg agagtacaga aacgccgtac cgcggcatta
    27661 ataaacgagg taaccagcag cacagacaac ctgataaaac tgtttcgt
//
LOCUS       DAZUCE010000041        27311 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZUCE010000041 DAZUCE010000000
VERSION     DAZUCE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.041"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            129..2309
                     /gene="katG"
                     /locus_tag="ACHY03_001508"
     CDS             129..2309
                     /gene="katG"
                     /locus_tag="ACHY03_001508"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFS3904755.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     VLPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFTPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            2403..3308
                     /gene="yijE"
                     /locus_tag="ACHY03_001509"
     CDS             2403..3308
                     /gene="yijE"
                     /locus_tag="ACHY03_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFS3904756.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAAVFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQGEIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(3335..3952)
                     /locus_tag="ACHY03_001510"
     CDS             complement(3335..3952)
                     /locus_tag="ACHY03_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFS3904757.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAIPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     DYPQKWKLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(4227..5330)
                     /gene="gldA"
                     /locus_tag="ACHY03_001511"
     CDS             complement(4227..5330)
                     /gene="gldA"
                     /locus_tag="ACHY03_001511"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFS3904758.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENSPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMQIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(5341..6003)
                     /gene="fsa"
                     /locus_tag="ACHY03_001512"
     CDS             complement(5341..6003)
                     /gene="fsa"
                     /locus_tag="ACHY03_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS3904759.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(6015..8516)
                     /gene="ptsP"
                     /locus_tag="ACHY03_001513"
     CDS             complement(6015..8516)
                     /gene="ptsP"
                     /locus_tag="ACHY03_001513"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS3904760.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPRIIRARTVCSGSAGGILTPISSLDLNALGNLPAANGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWARESVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            8825..9904
                     /locus_tag="ACHY03_001514"
     CDS             8825..9904
                     /locus_tag="ACHY03_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFS3904761.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            9919..10239
                     /locus_tag="ACHY03_001515"
     CDS             9919..10239
                     /locus_tag="ACHY03_001515"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFS3904762.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            10290..12587
                     /locus_tag="ACHY03_001516"
     CDS             10290..12587
                     /locus_tag="ACHY03_001516"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFS3904763.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            12553..13431
                     /locus_tag="ACHY03_001517"
     CDS             12553..13431
                     /locus_tag="ACHY03_001517"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFS3904764.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVMENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVLAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            13433..13774
                     /locus_tag="ACHY03_001518"
     CDS             13433..13774
                     /locus_tag="ACHY03_001518"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFS3904765.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGISIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(13761..14612)
                     /locus_tag="ACHY03_001519"
     CDS             complement(13761..14612)
                     /locus_tag="ACHY03_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3904766.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTAKLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGTIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(14832..16565)
                     /locus_tag="ACHY03_001520"
     CDS             complement(14832..16565)
                     /locus_tag="ACHY03_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFS3904767.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQMGNQ"
     gene            complement(16748..19399)
                     /gene="ppc"
                     /locus_tag="ACHY03_001521"
     CDS             complement(16748..19399)
                     /gene="ppc"
                     /locus_tag="ACHY03_001521"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFS3904768.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRKAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(19997..21148)
                     /gene="argE"
                     /locus_tag="ACHY03_001522"
     CDS             complement(19997..21148)
                     /gene="argE"
                     /locus_tag="ACHY03_001522"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFS3904769.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            21302..22306
                     /gene="argC"
                     /locus_tag="ACHY03_001523"
     CDS             21302..22306
                     /gene="argC"
                     /locus_tag="ACHY03_001523"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFS3904770.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            22314..23090
                     /gene="argB"
                     /locus_tag="ACHY03_001524"
     CDS             22314..23090
                     /gene="argB"
                     /locus_tag="ACHY03_001524"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFS3904771.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            23151..24524
                     /gene="argH"
                     /locus_tag="ACHY03_001525"
     CDS             23151..24524
                     /gene="argH"
                     /locus_tag="ACHY03_001525"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFS3904772.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHMQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSLQQVAQAIA
                     FARARLG"
     gene            24791..25708
                     /gene="oxyR"
                     /locus_tag="ACHY03_001526"
     CDS             24791..25708
                     /gene="oxyR"
                     /locus_tag="ACHY03_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFS3904773.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(25691..27091)
                     /gene="sthA"
                     /locus_tag="ACHY03_001527"
     CDS             complement(25691..27091)
                     /gene="sthA"
                     /locus_tag="ACHY03_001527"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFS3904774.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARIAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         6171 a         6900 c         7636 g         6604 t
ORIGIN      
        1 atttgatcta catctcttta accaacaata tgtaagatct caactatcgc atccgtggat
       61 taattcaatt ataacttctc tctaacgctg tgtatcgtaa cggtaacact gtagagggga
      121 gcacattgat gagcacgtca gacgatatcc ataacacgac agccactggc aaatgcccat
      181 tccatcaggg cggtcacgac cagagtgcgg gggcgggcac aaccactcgc gactggtggc
      241 caaatcaact tcgtgttgac ctgttaaacc aacattctaa tcgttctaac ccactgggtg
      301 aggactttga ctaccgcaaa gaattcagca aattagatta ctacggcctg aaaaaagatc
      361 tgaaagccct gctgacagaa tctcagccgt ggtggcctgc cgactggggc agttacgccg
      421 gtctgtttat tcgtatggcc tggcacggcg cgggtactta ccgttcaatc gatggacgcg
      481 gcggcgcggg tcgtggtcag caacgttttg caccgctgaa ctcctggccg gataacgtaa
      541 gcctcgataa agcgcgtcgc ctgttgtggc caatcaaaca gaaatatggt cagaaaatct
      601 cctgggccga cctgtttatc ctcgcgggta acgtggcgct ggaaaactcc ggcttccgta
      661 ccttcggttt tggtgccggt cgtgaagacg tctgggaacc ggatctggat gttaactggg
      721 gtgatgaaaa agcctggctg actcaccgtc atccggaagc gctggcgaaa gcaccgctgg
      781 gcgcaaccga gatgggcctg atttacgtta acccggaagg ccctgatcac agcggcgaac
      841 cgctttctgc ggcagcagct atccgcgcga ccttcggcaa catgggtatg aacgacgaag
      901 aaacagtggc gctgattgcg ggtggtcata cgctgggtaa aacccacggt gctggtccga
      961 catcaaatgt aggtcctgat ccagaagctg caccgattga agaacaaggt ttaggttggg
     1021 cgagcactta cggcagcggc gttggcgcag atgccattac ctctggtctg gaagtagtct
     1081 ggacccagac gccgacccag tggagcaact atttcttcga gaacctgttc aagtatgagt
     1141 gggtacagac ccgcagcccg gctggcgcaa tccagttcga agcggtagac gcaccggaaa
     1201 ttatcccgga tccgtttgat ccgtcgaaga aacgtaaacc gacaatgctg gtgaccgacc
     1261 tgacgctgcg ttttgatcct gagttcgaga agatctctcg tcgtttcctc aacgatccgc
     1321 aggcgttcaa tgaagccttt gcccgtgcct ggttcaaact gacgcacagg gatatggggc
     1381 cgaaatctcg ctacatcggg ccggaagtac cgaaagaaga tctgatctgg caagatccgc
     1441 tgccgcagcc gatctacaac ccgaccgagc aggacattat cgatctgaaa ttcgcgattg
     1501 cggattctgg tctgtctgtc agcgagctgg tatcggtggc ctgggcgtct gcttctacct
     1561 tccgtggtgg cgacaaacgt ggtggtgcca atggtgcacg tctggcatta atgccgcagc
     1621 gcgactggga tgtgaacgca gcagccgttc gtgtgctgcc agttctggag aaaattcaga
     1681 aagagtctgg taaagcctcg ctggcggata tcatcgtgct ggcaggtgtg gttggcgttg
     1741 agaaagccgc aagcgccgca ggtttgagca ttcatgtacc gtttacgccg ggccgcgtcg
     1801 atgcgcgtca ggatcagact gacattgaga tgttcgagct gctggagcca attgctgacg
     1861 gtttccgtaa ctatcgcgct cgtctggacg tttccaccac cgagtcactg ctgatcgaca
     1921 aagcccagca actgaccctg accgcgccgg aaatgaccgc gctggtaggc gggatgcgtg
     1981 tactgggtgc caacttcgat ggcagcaaaa acggcgtctt cactgaccgc gttggcgtat
     2041 tgagcaatga cttcttcgtg aacttgctgg atatgcgtta cgagtggaaa gcgaccgacg
     2101 aatcgaaaga gctgtttgaa ggccgtgacc gcgaaaccgg cgaagtgaaa tacactgcca
     2161 gccgtgcgga tctggtattt ggttctaact ctgtcctgcg tgcggtggct gaagtttacg
     2221 ccagcagcga tgctcacgag aagtttgtta aagacttcgt ggcggcatgg gtgaaagtga
     2281 tgaacctcga ccgtttcgac ctgctgtaat ctgaccttct tcagcgactg cctttcaggc
     2341 agtcgctgaa gtttctttac cggcgtatag tgtccacagg aaaactacac actggatctc
     2401 tcatgtctgc cgcaggaaag agcaacccac tggcaatcag tggcctggtt gtgctcacac
     2461 ttatctggag ttatagctgg attttcatga agcaagtcac cagctacatc ggtgccttcg
     2521 actttaccgc cttacgctgc attttcggcg ctctcgtttt attcatcgtc cttttattac
     2581 gtggtcgcgg aatgcgcccg acaccgttta aatacacctt agccattgcc ctgttacaaa
     2641 cctgcgggat ggttggcctg gcgcagtggg cgctggtcag cggcggagcg gggaaggtgg
     2701 cgatcctgag ctataccatg ccgttctggg tggtgatttt cgccgcggtg tttctcggtg
     2761 aacgcctgcg acgtgggcaa tatttcgcga ttctgattgc cgcttttggc ttatttttgg
     2821 tgttgcagcc gtggcaactc gatttctctt cgatgaaaag tgccatgctg gcaatcctct
     2881 ccggtgttag ttggggggcg agcgcgattg ttgctaaacg tctgtatgcc cgtcatccgc
     2941 gcgtggattt attgtcgtta acatcctggc agatgctcta tgcggcgcta gtgatgagtg
     3001 tggtcgcttt actggtgccg caaggtgaaa ttgactggca gcccactgtg ttctgggcgc
     3061 tggcttacag tgcgattctg gcgacggcgt tggcgtggag cttatggttg tttgtattga
     3121 aaaacttgcc tgccagtatt gccagcttaa gcacactggc cgttcccgtt tgcggcgtac
     3181 tcttttcctg gtggctgctc ggtgagaatc cgggggccgt tgaaggtagc ggtattgtgc
     3241 tgattgtgct ggcactggcg ctggtgagcc gtaagaaaaa agaagccgtt agtgtaaaaa
     3301 ggatctgaat tttttcttca tgtggggcga tcgcttattt aacaaaataa cgataatgcc
     3361 ccaccatgcg ccagctaaac agcacatctt cttcctgcgc acctgcgcca atgttatgta
     3421 tcaccagcgg cgtaccgtcg cgggcgaagc catctgaaac caccccaata tgtgccagcc
     3481 cgttatctaa tcgccaggag acaatatcgc ccgcttgata gtcactgggg ttcttgctga
     3541 tggggcgtgt tttatcgtgg cgggtaaacc aggtttccag attaggcacc cgacggtgat
     3601 cgatgttgct gtccgggcgc tttaacttcc acttttgcgg ataatcggca aaattcttcg
     3661 ccatatcttc gtgaaccagt ttctgcaaat cgaccttctg gctgcgcaat gcgcggatca
     3721 ccacatcgga acatacgccg cgttcttgcg ggacatcacc gccaggataa gtaagctgca
     3781 catacgccgg atcgtaaaat agcgtgctgc caatttgctg tctggcaccg tctgcgatgg
     3841 caaggttggt attggcctgg atttgtacca cggttggtgg aatggcggga gattttaagg
     3901 agtggctggt aaatgccgtt agcaggctga gcagcgccag tgaagctttc atctggttcg
     3961 tccgtgaaaa taagagtcac agattcgacc ttccgggcta acgattgttc tgtagagaaa
     4021 atgtccagaa gcggataaaa aattcctgaa caacgtggga gtttcagatt actggcacag
     4081 agtgccggat gcggagtgaa cgccttattc ggcctacaaa agcacgcaaa ttcaatatat
     4141 tgcagagatt gcgtaggcct gataagcgta gcgcatcagg caattttgcg ttcaaactcc
     4201 cggacaagcc gggagtttgg agtaggttat tcccactctt gcaggaaacg ctgaccgtac
     4261 tggtcggcta ccagcagagc ggcgtaaacc tgatctggcg tcgcgccgcc aggcatgttg
     4321 tggatggttt caccttctgc acatgccgct tctgccacaa tttgcatttt cgccgggaca
     4381 tcttctttaa tatccagttg agcgagagtt attggcaaac ctaccgcatg gctaagcgca
     4441 gctacggttt cgatttcctc aaccggcgag ttttccagta ccagctgcgt cagtgtacca
     4501 aatgccactt tttcaccgtg ataatagtga tgggcatcag gaatggcagt caggccatta
     4561 tgcaccgcgt gcgctgccgc cagaccaccg ctttcaaagc caacaccgct taaataagtg
     4621 ttcgcttcaa tcacgcgttc cagtgccgga gtcactacat gctgttcggc ggcgagcatc
     4681 gctttttcgc cttcttccag caaggtgttg tagcacagtt cagccagcgc cagcgccgcc
     4741 tgagtgcact tgccgcccgc catggtggtc gcaccgctac gagagcaggc acgcgcttca
     4801 aaccaggtcg ccagcgcatc gccgataccc gccgctaaca gacgtgcagg tgcgccagcg
     4861 acgattttgg tgtcgacaat gaccatattc gggttatttg gcaacagcag atagcggtca
     4921 aactcaccct catcggtgta gataacagac aatgcgctgc acggtgcatc ggtagaggcg
     4981 atagtcggtg cgatcgctac cggaacaccc atgaaatgtg ccagtgcttt ggcagtatcg
     5041 agagtttttc cgccaccgat accgagaatt gcgccacact gcgcagtctc cgcgatgcca
     5101 cgcagacggt cgatctcatt ttgcgaacat tcaccgccaa acggcgcaat ttctactacc
     5161 agtccagcat ctttaaagct tttctcgaca gtggattgag caaaacctaa aacaaatttg
     5221 tcacccacca ctaaccagcg ttctgccagc ggcttcaggt attcgcccag acgattaatc
     5281 acatcagcgc cctggatgta tttacccggt gattgaataa tgcggtccat aattgctcct
     5341 ttagagatga gtagtgccaa atgcggcatt ccagtcgtgt tcgaacttct ctatagctga
     5401 ctctaccgca ggggtgttga gcatttgttg cgctacatct aagggcaggg tgatggattc
     5461 acatcctgcc agtaaacagt ccagcgcctg acgcggcgtt ttaaagctgg ccgccagcac
     5521 catgctttct ggtgcgtgca tttctaacag cgcttgcagc tcctgaaccg tacgaatgcc
     5581 gtccccgccc tgggcatcta cgcggttaac atacggagca acgtattttg cccctgccag
     5641 tgcggctaat aacccttgtg cggcgctata tacagcggtg ccaagtgtag taataccctc
     5701 ttttttcagt attttaattg ctgccagacc ttcggaagtc accgggattt tcaccacaat
     5761 gcccggaatg gcgtcgcgca gacgcttcgc ttctttcacc atcccctgcg cgtcgcggct
     5821 catggtctga gcaaacagaa tgccctcatc accaatcgct ttttgcagac gcggcagcac
     5881 ttcccagatg gactccttgc tggcagcgat aatgctcggg ttagttgtca ccccggcaat
     5941 cgggaatatg cgtgccagac gttcgacttc tgcgacgtta gcggtgtcca gatacagttc
     6001 catgatgttt ccttttacag ttccagttca tgttgcagca ggctggcgat agcgtcggca
     6061 gaggcggcgt taaccagcgc gttacggaat tcttcgtgca tgatgcggcg agcgaggcgc
     6121 gagaaaatgc gcatgtgctg atcgcccgca gcgtgtttgt tcagggttaa cataatgatg
     6181 aattgcgctt catcatcgcc ccagcgcacc ggcgcttgca gacgcgctac gctgatggtg
     6241 gattgctcaa tgtgttctga tttgctgtgg ggaatggcaa aactaaagcc cagaccggta
     6301 gagaaaacgg actcgcgcgc ccacaagtcg gcttccagtt tacgcggata gcggcagcgg
     6361 cccgccagca gcaggttatc ggtcatgcct ttgagtactt cttctttgct gcgccagtcg
     6421 ctttccagcg tgatgcactc ggcggtgacc agtggtgcgt cctgttgggt catgcggaat
     6481 tgcgccagca gatgttccac ttccagcgaa gtacggcagg ccattgcctg gttgagcaac
     6541 tggcggcact cacggctatc aagttgcgcc attcgcgctt tcgccgccgg aattgacggt
     6601 gcgctcatgc tgagttcatc cagccctaaa ccgaccagca acggcagcac ggaacctttc
     6661 gctcccagct caccgcacag accaatccat ttgccctggc gatgcactgc ttgcacggcg
     6721 taatcgagcg cccgcaagaa tgccggattc aggctgttgt agtgacgagt aaccttagcg
     6781 ttatcgcgat ccaccgccag cagatattgc gtcaggtcat tactaccaat actaaagaaa
     6841 tcaatctctt cgcagcattg atcgatgatg aacatcaccg acggcacttc cagcataatg
     6901 ccgagctgga tcttctcatc aaacggaatg tgttcgttac gcagttgctg tttggcttcc
     6961 gccagttttt ctttcaccca taaaatctct tccattgaag agatcatcgg gatcatgatt
     7021 ttcaggctgc cgtgagcgga ggcgcgcagg atcgaccgta gttgtgtggt gaacagcgag
     7081 gcgtattctt cataaatacg cacagcgcga taaccgagga acgggttagc ttctgccgga
     7141 atgttcagat aatcaacggg tttgtcgccg ccaatgtcca tagtgcgcac aataatgctg
     7201 cgtccgttgg cggactccag cgcctgacaa aaaatgttgt acagctcgct ttcgcccggt
     7261 gcactggtgc gatccatata gagcatttca gtgcggaaca aaccaacgcc ttccgcacca
     7321 ttgccgaatg cggcctgcgc ttccacggag tgagcgatgt tagcggcaat ttcaatgcgg
     7381 ataccgtcag cggtacgggc ttgttgggtc agccagacac gctgttgctc acgaagggcg
     7441 tcctgtacgc gggcttcttg ctgataataa cgagctacgg cttcccctgg ctcaaccaca
     7501 atcgccccgg cgttgccgtc gatataaatc gtttgatgct gccacggagt aagggcatca
     7561 atatccacac caaccagcgt cggaatgttg aacgaacggg caaggatcac cgtatgtgac
     7621 gtggtgccgc cgcttttgag caacaatcct ttgaggtgat ttttatccag ttcgaggaac
     7681 tggctgggag ttagttcatc agccatacat atggcgggct gcgtcagttt gcccggtgcc
     7741 gggaagcgtt gctcaccgta gatttgctgg agtaactgga agcagacgtc gcgtacgtcc
     7801 agggcacgtt cttgcaggta gctgctgctg gaacgggcga actcttcgca aaagtgattc
     7861 gcactggcga caatcgcttc ggcgcagctt aatccggcgc tgacacctgc cagtaaatgt
     7921 tcgcgcaggg aagtatcgcc agccagggat cggtgcgctt ccagaatcgc gctggtagca
     7981 ccgtcgctat ccagcagacg aaactcaatg tttttcagta ccagcgtcag gccgttttcc
     8041 agcgcggatt gctcggcgtc aacgccgttg gcggcgggaa gattacccag cgcgttgaga
     8101 tctaaagaag agatcggcgt cagaatgcca cctgcgctac cgctgcacac ggtgcgggcg
     8161 cgaataatgc gcggattcaa attagtgagt gaaaccggca gcggctccag ctcgtcagat
     8221 tttacttccg ccagcggtgc gtcgcagtgg gggaattcat cgcgcagcca ttggcttaaa
     8281 cgctggtgcg cttcctgttc gtcggccccg gaaatcagta actggcagtt atcgcccacc
     8341 agcgtatcgg tgccaatcag cgcaagggcg cttttggcgt tgcccttgcg atcagtgcgc
     8401 aggttatgcc actcaatttg tgatgaaaaa gtattacaca gcgtttcaac gtggcttgcc
     8461 ggacgtgcat gtacgccgtt aggtagctca caaataaatt ccacaatcag ggccattgcc
     8521 tctctcccgg tacggttttc tgtttacagg ataagagcgc acggcaacgg cctgccatgt
     8581 gacaaatctg ccaaaagctg gacaaatgta atgtaaccgt caatttgcga cgcgtctcac
     8641 aagacgccgt tttgcggcat gcttccggat tatcgcaagt tatgaggcgg atcgcatttt
     8701 tgtactgata ttacaaaagt ccagtaaatg gcctttttat ccactgtttg ctccgcctgc
     8761 gattgcctat tgtttcctcc atcaaaaata tggacatggg ctttcgcaag cccgggagca
     8821 gcttatgaat gagttggtgc agatcctgaa aaatacccgt cagcatttaa tgacgggcgt
     8881 atcacacatg attcccttcg tggtatcggg cggtattttg ctggcggttt ccgtcatgtt
     8941 gtatggcaaa ggcgcagtgc cggatgccgt agccgatccg aatttgaaaa aactgtttga
     9001 tatcggcgtt gcaggtttga cgctgatggt gcctttcctc gccgcttaca ttggttactc
     9061 cattgcagag cgttctgcgc tggccccgtg cgctatcggt gcctgggttg gtaacagctt
     9121 tggtgcgggc ttctttggtg cgctgatcgc cgggattatc ggcggcatcg tggtgcatta
     9181 cctgaagaaa attccggtgc ataaagttct gcgctcggtg atgcctatct tcattattcc
     9241 tatcatcggc acactgatta ccgcaggcat catgatgtgg ggcttgggcg agcctgtagg
     9301 ggcgttgacc aacagcctga ctcagtggct tcaggggatg cagcagggca gcattgttat
     9361 gctggcggtg atcatgggac tgatgctggc gttcgatatg ggcggtccgg ttaacaaagt
     9421 ggcctatgcc ttcatgctga tttgcgttgc tcagggggtt tataccgtgg tggccattgc
     9481 tgccgtgggt atttgtatcc cgccgctggg gatggggctg gcgacgctga ttggtcgtaa
     9541 aaatttctcc gcagaagagc gcgaaaccgg taaagcggcg ctggtgatgg ggtgcgttgg
     9601 ggttactgaa ggggcgattc ctttcgccgc agccgatccg ttacgtgtaa ttccttccat
     9661 catggttggt tcagtttgtg gtgcagtaac tgcggcgctg gtcggtgcgc agtgctatgc
     9721 aggctggggt ggtctgattg tgctgccggt ggttgaaggc aagctgggtt atatcgcagc
     9781 agtggctgtc ggagcagtgg tgacggctgt ttgtgtgaac gtgctgaaaa gtctggcgcg
     9841 taaaaatggg tcttcgactg atgaaaaaga agacgacctg gatttggatt ttgaaattaa
     9901 ttaattgagg aacgagccat gacgaaaatt attgcagtaa ccgcgtgtcc atctggtgtt
     9961 gcccatactt atatggcggc agaggcgctg gaaagtgccg cgaaagcgaa aggctgggaa
    10021 gtgaaagtag aaacccaggg gtcaattggt ctggaaaatg aactgacagc ggaagacgtg
    10081 gcgagcgccg atatggtgat tctgaccaaa gatatcggca tcaagtttga agagcgtttt
    10141 gctggcaaga ccatcgtgcg cgtcaacatc agcgatgcgg ttaaacgcgc tgacgccatt
    10201 atgagcaaaa ttgaagctca cctggcgcaa accgcttaag ttcttatccc gcccgtaagg
    10261 gcgggttcgc tttcccacag gagttcctca tgacgaatcg tatctctcgc ctcaaaactg
    10321 cactgtttgc caatacccgt gaaatctcgc tggagcgtgc gctgctttat accgccagcc
    10381 atcggcaaac cgaaggcgaa ccggtgatat tgcgccgggc gaaagcaaca gcgtatatcc
    10441 ttgaacatgt tgaaatttcg attcgtgatg aagaactgat tgccggtaac cgcaccgtaa
    10501 aaccgcgcgc cggaattatg tcgccggaaa tggaccctta ctggctgctg aaagagctgg
    10561 atcaattccc gacgcgtccg caggaccgct ttgctatcag cgaagaagat aaacgtatct
    10621 accgcgaaga gttgttcccg tactgggaaa aacgttcgat gaaagatttc attaacgggc
    10681 agatgacgga tgaagtaaaa gccgcgacca gcactcagat tttcagcatc aaccagacgg
    10741 ataaaggcca ggggcacatt attattgatt acccgcgcct gctgaatcac gggctgggtg
    10801 agctggtagc gcagatgcag caacattgtc agcaacagcc ggagaatcac ttttatcagg
    10861 cagcgctgtt actgctcgaa gcctcgcaga aacacatttt gcgttacgcc gaactggcgg
    10921 aaacgatggc ggcaagctgc actgatgccc agcgtcgcga agaactgctg actattgcgg
    10981 agatctcccg ccataacgcc gaacataagc cgcagacgtt ctggcaggcg tgccagttat
    11041 tctggtacat gaacatcatt ctgcaatacg aatccaacgc cagttcgctg tctttagggc
    11101 gcttcgacca gtatatgttg ccgttctatc aggcatcttt aacccagggc gaagatccgg
    11161 cgttcctgaa agaactgctc gaatctttat gggtgaaatg caacgacatt gtgctgttgc
    11221 gctccaccag cagcgcgcgt tatttcgcag gtttcccgac tggctatacc gcactgctcg
    11281 gcgggttaac cgagaacgga cgtagcgcgg tgaacgtgct ttcgttcctc tgcctcgatg
    11341 cctatcaaag cgtacaatta ccgcaaccga acctaggggt gcgcactaac gccttgatcg
    11401 acacgccgtt cctgatgaaa accgcagaaa ccattcgcct cggcaccggt attccgcaaa
    11461 tctttaacga tgaagtggtg gtgccagcgt tcctcaaccg tggcgtttcg ctggaagatg
    11521 cgcgcgacta ttccgtagtg ggctgtgtgg aattatctat tcccggcaga acctacggct
    11581 tgcatgacat cgcgatgttt aatctgctga aagtgatgga aatctgcctg catgaaaatg
    11641 aaggcaatgc tgcgctgact tatgaaggtt tactggagca gatccgcgcc aagatcagcc
    11701 actacatcac cctgatggtt gagggcagca atatttgtga tatcggccat cgcgactggg
    11761 cacctgtacc gctgctctcg tcttttatca gcgattgtct ggaaaaaggt cgcgatatta
    11821 ccgatggcgg cgcgcgttat aacttctccg gcgtacaggg aattggtatc gccaacctga
    11881 gcgattctct ccatgcgttg aaagggatgg tttttgatca acagcgttta agttttgacg
    11941 aattgctgtc ggtattaaaa gccaactttg caacgccaga aggcgaaaaa gtccgcgctc
    12001 gcttaattaa ccgctttgag aaatacggta acgatatcga cgaggtggat aacattagcg
    12061 ccgaactgtt gcgccactac tgcaaagaag tggaaaaata ccagaacccg cgcggcggtt
    12121 acttcacgcc gggatcgtat accgtttctg ctcacgtccc gttgggatcg gtggttggcg
    12181 cgacgccaga cggtcgtttt gccggagaac agctggcaga cggcggcttg tcacccatgc
    12241 tgggccagga cgcacaaggg ccaacagcgg tactgaagtc agtcagtaag ctcgataaca
    12301 cgctgctgtc taacggtacg ttgctgaacg tgaaattcac tccggcgacc ctggaaggtg
    12361 aagcgggatt acgcaaactg gccgacttct tacgggcgtt tacccagctt aagttgcagc
    12421 atattcagtt taacgtggtg aacgccgaca cgttgcggga agcacaacag cgcccacaag
    12481 attatgccgg gctggtggtg cgcgttgccg gatacagcgc cttctttgtc gaactgtcga
    12541 aggagatcca ggatgacatc atccgccgga cagcgcatca gctgtaacgt tgtggaaacg
    12601 cgccgcaatg atgtggcgcg tattttcaac attcagcgtt attcactgaa tgacggcgag
    12661 ggcattcgta cggtggtctt ttttaaaggc tgtccgcatc tttgcccgtg gtgtgctaat
    12721 ccggagtcga tctccggcaa aatccagacg gtacgcagag aggcgaaatg tctgcactgt
    12781 gcgaaatgtt tgcgtgatgc ggatgaatgc ccctccgggg cgtttgaacg gattggtcgc
    12841 gatatcagcc ttgacgcgct ggaacgggaa gtgatgaaag atgacatttt ctttcgcacg
    12901 tccggcggcg gcgtcacgct ttctggcggc gaagtgttaa tgcaggcgga gtttgctacc
    12961 cgttttttac agcgactgcg gctgtggggt gtgtcatgcg ccattgaaac tgccggagac
    13021 gcaccagcca gcaagctgtt accactggcg aaattgtgcg atgaagtgtt gttcgattta
    13081 aaaatcatgg atgcgactca ggcgcgggat gtggtgaaga tgaacctgcc acgcgtgatg
    13141 gagaatctgc gtttgctggt gagtgagggt gtcaacgtga tcccacgttt accgctgatc
    13201 cctggtttca cgctcagccg ggagaatatg cagcaggcgc tggatgtact gatcccgctg
    13261 aaaatcaggc agatccatct gttaccgttt catcagtacg gcgaaccgaa ataccgcctg
    13321 ctggggaaaa catggtcgat gaaagaggtg cttgcgccgt cgtcagccga tgtgacaacg
    13381 atgcgcgaaa tggcagaacg ggccggattt caggttaccg tgggaggtta aaatggcata
    13441 cctggtggca gtaaccgcct gcgtcagtgg cgtggcgcat acttatatgg cggcggaacg
    13501 gctggaaaag ttgtgcctgt tagagaagtg gggcatcagc attgaaactc agggcgcgct
    13561 gggaacggag aatcgtttag cggacgagga tatccgtcgg gcggatgttg ctctgttgat
    13621 tacggatatc gagcttgccg gtgccgagcg atttgaacat tgccgctatg tgcaatgcag
    13681 catctacgca ttcctgcgtg agccgcagcg ggtaatgagc gcggtgcgca aagtgctttc
    13741 tgcgccgcag caaacccatc ttattctgga gtagtcggtt tttctgtcag ttggctgtgg
    13801 tactgccgac gatattccga cggcgagcgt tcggtgtttt tacgaaacag acggcagaag
    13861 tagttgctgt cgacaaagcc gcaggcgtgc gccacttctt ttaccttcag gtcatagcct
    13921 ttcaataacg tcttagcgtg ctccagtcgc gtgtgattca ggtattcgtt aaaaccaatg
    13981 gtccccgttt tttgaaacag gtgcgagaga tagtttggtg aaatatagaa tgcctgtgcg
    14041 acagattcgc gggtaagcgc ggaggcatag cgttcgtcga tataatcacg aatagcttcg
    14101 aataatgcct ggctgcgtga ggcggtctgg atttggctgc cgagcaaatc gcggcagtgg
    14161 ctaagcaggc tggcgacgat aagctttgcc gtttgctgct cctgcggctg catttgcatt
    14221 tcattgagcg tttgtagcag aaaagaacct atgcgtgggc cgcgccgggc gacgtgttgc
    14281 ttcgccagat tttgatattg tttgccatcc cattgcacga cgctgaagcc gagttgctgt
    14341 ttgccaaaca gcacgctaaa ggtagtggcg ggggcttgcc attgtggaaa attccagcca
    14401 ccagcggcaa cgtacagcac atcgccggga actaacgttg ctccagcgcc ggtatcaaca
    14461 aactcacctt ccagcacaat ttccagccgt ggaaaatcga cctgatacgc aagatcgggc
    14521 accgggccgt tactgctggc aaagtaaatc tggcgcaggg acagcgggcc gttgatcagg
    14581 cgagagagca gatagctgac gtcgtgatac atgttattcc cttaggtatg tagacgggaa
    14641 aacagtaact ggcattatta aaaaaggcca cctgaaaagt gtggtgtggc ctgaggggag
    14701 ttcgatgtaa acattgcggc agttgtattg ccggatgtgg cgtaaacgcc ttatccagtc
    14761 taccgccgcg atctgtaggc cggataagac gcgtcaagcg tcgcatccgg caaatagcgc
    14821 ctggctgata attactgatt acccatctga tcgccatacg gcagcgtgtc gtaatcgatc
    14881 agcgcgtttt tcttgtacgg gttaccaatc cagcgggtag tttctttgaa ctgcggattc
    14941 accactgaac gggttggatc gtaaccgtcg taagataagc ccgccaaatc tgaccaggtg
    15001 tggatcagtt ccgccaggct gtatttacga tcaacatcct gtgagaaatc acgcggatga
    15061 gtcgcttgcc atttttccga cgtccacagc aggaacggaa tggtatacat atgacgcgtc
    15121 gggttgtctt cgttacgccc ctgagttttg tgcggcggtg tgtcgtaaac ctcttcaccg
    15181 tggtcagaga agtaaaccag gaatccgttc gggtctgccg ctttaaagtc tttaatcagg
    15241 ctggcaacca catgatcgtt atacaggtta gcgttgtcgt aatcgttata tgactccagc
    15301 tcttctgcgc ttaatcccgg tggaacatga tcggtattgc catcaaactt gccctgattt
    15361 tccgggtagc ggtatttgta tttgatatgc gtacccagca gatgaacaat gatcagtttc
    15421 ttcggcgcag ggtcattcag cacttcctgg aacggcttca gcacgttagt gtcgtattca
    15481 cgcgcactct gcgtacgttg ctggttcatg tagtactgct tgtcggtctg gcgcgaaaat
    15541 accgtcagca tggtattgcg ggcggtcatc gtctgctggt tggtgatcca gaaggtttta
    15601 taacccgcct gtttcatcat gttcatcagc gacggctgcg tcagatacag atccgggttc
    15661 ttttcattgg caaaggtcag cgcctgttgc aggatttcaa tggtgtacgg acgagacgta
    15721 actacgttat taaacacggt tagattcgga tcggttttat gcagcgcatc cagctccggc
    15781 gtggtttcac gcggataacc gtacagactc atgcgtccac gctgggttga ctcgccaatc
    15841 accagcacta aggtgcgcgg ttcgttaccc gattcatctt tgaaattagc cagcggcggc
    15901 aaggcattat tttcattcag tagctttgtt agcgagttta gttgctgacg atactgataa
    15961 tagccggtca ggaattgcca cggtgcggca ggctccatac gcgaggccag gttatccaac
    16021 gttttctcaa acggcttgtt tttgataaac gtattcatgg cgatcggatg cagaatcaag
    16081 ccataaagca gggcaaaaga gacaacataa cgccacggct ttggaatata gaccggacgc
    16141 aggcgtgtcc acagcagaac tgccaccgcc gtataggcca gcgcgataag cacaattttc
    16201 aggctgaaat actggcttaa atactcgctg gcttcgttgg tgttggtttc gaacatcaca
    16261 aacagaacgc tctgcgagaa ctcctgaccg tagatgacgt agtagcacag cgccgccaga
    16321 gaggccgccc atagcaccac gccgatcact gcggcaataa ttttgatccg cttcggaaag
    16381 aggaataccg gaattaacca cagcgagctg aataacagcg agtcgcgaat gccgttagtg
    16441 ccactataac cactgatgta aataatggcc tgtagcagag tagagaaaaa ccaaaagtag
    16501 agcagtgccc aacccagggc tttccagcta aaaagaggtt tagcctggac ttctgtggaa
    16561 tgcatagtaa gaacctgtct tgaaaaaaat atcgccgaat gtaccgacaa ttccttaagg
    16621 atatctgaag ggatatccag aatttgaata aaatgcagac agaaatatat tgaaaacgag
    16681 ggtgttagaa cagaagtatt tcagaaaacc ctcgcgcaaa agcacgaggg tttgcaggag
    16741 aggaagatta gccggtatta cgcatacctg ccgcaatccc ggcaatagtg accattaacg
    16801 cctgctcgac gcgaggatcc ggttcctgcc cttctttttc tgccttgcgg gagcggtgca
    16861 gcaactcggc ttgcaatacg ttcagcgggt cggtgtaaat attccgtagc tgaatagact
    16921 ctgcaatcca cggcagatcg gccatcagat gggaatcgtt ggcaatcgcc agcaccactt
    16981 tgatgtcttc ttcttgcaga ttgcgtaact ctttacctaa cggccacagt gctttgtcta
    17041 ccaggcgttg gtcatagtat tccgccagcc acaggtctgc tttggcgaag accatctcca
    17101 gcatgccgag acgcgtcgag aagaatggcc aatcgcggca catagcctcc agctcgctct
    17161 gtttgccatc ttcgaccact ttttgcagcg ccgtacctgc acccagccag gcggggagca
    17221 tcagacggtt ttgcgtccag gcgaagatcc acggaatggc gcgtaatgac tcgacgccgc
    17281 cggttgggcg acgtttcgcc ggacgtgaac ccaacggtag tttgcccagt tcctgttccg
    17341 gcgtagcgga gcggaagtaa ggcacaaaat ctttgttttc acgtacgtag ccacggtaga
    17401 gatcgcagga gatgactgat agttcatcca taatgcgacg ccagctctct ttcggctcag
    17461 gtggtggcag caggttggct tccagaatcg ccccggtgta aagcgacagg ctgctgacgg
    17521 tgatttctgg cagaccatat ttaaagcgga tcatctcgcc ctgttcagtc acgcgcaggc
    17581 cacctttcag gcttcctggc ggttgtgaca gcagcgccgc atgagcaggt gcgccgccgc
    17641 gaccaatgga accgccgcga ccgtggaaca acgtcagctc aatacccgct ttttcgcagg
    17701 ttttgattaa tgcatcctgt gcctgatatt gcgcccagga cgctgccatc acgcccgcat
    17761 cttttgctga gtcggaatag ccaatcatca ccatctgttt gccctgaatc aggccgcgat
    17821 accagtcgat attcagcaac tgagtcatga catcgttggc gttgttcagg tcatcgagag
    17881 tttcaaacag cggagcaacc ggcatcgcaa acccgatacc cgcttctttc agcagcaggt
    17941 ggacagccag cacgtcggac ggcgttttcg ccatcgagat cacgtaggcg gcaatggagc
    18001 cttgcggtgc ttcggcaatc acctggcagg tatcgagcac ttcgcgcgtt tcggcgcttg
    18061 gttgccagtt gcgcggcaga agcggacgtt tggagttcag ttcgcggatc aggaacgcct
    18121 gtttgtcggc ctctgaccag ctttcgtagt cgccgatacc gaggtagcgg gtcagctcgc
    18181 ccagcgcttc ggtatggcgg gtgctttcct gacggatatc aatacggacc agcggtacgc
    18241 cgaaacattt cacgcggcgc agggtgtcga gcagatcgcc gttggcgata atacccatgc
    18301 cacacgcctg aagtgactgg tagcaagcgt agagcggttc ccacagttct tcgttttgtg
    18361 tcagcaggcc ttctggtttt ggcagttctt cgcctttcag gcgcgcttcc agccatgcct
    18421 gtgtcgccat caggcgagaa cgcaggtttt tcatcagata gcgatacggt tctgcggcac
    18481 cttcttcgcc aaccagcgcc agcagttcag gggtcgcttc aaccatcgac agttcagaaa
    18541 ccagaacctg aatatctttc aggaacagat cggtggcttt ccagcggctg agtagcagga
    18601 cgtggcgggt gatatcggca gtgacgttcg ggttgccgtc gcggtcaccg cccatccacg
    18661 aggtaaaacg gaccggaaca aattcgacgg gcagtttgta gccgaggttc tcttccagtt
    18721 gttcgttcag ttcgcgcagg taattcggta cgccttgcca caggctgttt tctactacgg
    18781 caaagcccca tttggcttca tctaccgggc ttggacgcag cttacggatt tcatcggtat
    18841 gccatgactg ggcgatcaac tggcgcaggc ggcgcatcag ctggttgtgt tcgtagtcag
    18901 cgatatcttt gttatcgagc tgttttaaac aggcgttcac ttccaccatt ttgtggatca
    18961 gtgtacgacg ggtaatttcg gttgggtgag ccgtgaggac cagttccagc gacagcgatt
    19021 ccactgcttt tttgatggtg tcttcgctca gttccggttg gtttttcagt ttgcgcaggg
    19081 tgcgggcgat cacttccggg ttgctggcag cttcgccttt cggcgaaatg ctgtggtatt
    19141 gctcggcggt gttggccagg ttcagaaact gactaaacgc acgtgcaacg ggcagcagct
    19201 cgtcgttcga caaattttgt aaggtggtga gtaactcctg gcggttagca tcattgccag
    19261 cgcgtgaaga tttggacaac ttacggatag tttctacgcg ttcaagaatg tgttctccca
    19321 acgcatcctt gatggtttct cccagcactt tgccgagcat actgacatta ctacgcaatg
    19381 cggaatattg ttcgttcata ttaccccaga caccccatct tatcgtttga tagccctgta
    19441 tccttcacgt cgcattggcg cgaatatgct cgggctttgc ttttcgtcgt cttttataaa
    19501 gccacgtaaa agcggtgacg tcaaatgctg cgaaatcgct tcagcaaacg aataaatagc
    19561 aggaatttac gtcattaaat tcacgacgct ttaaataagc gtaacttatg ggaatgttaa
    19621 aaaatcgcct caattaacac caaaggtgta ggtcggataa gatgcgcaag catcgcatcc
    19681 gacattattg cggcactgga gtttggcaac agtgccggat gcggcgcaag cgccttattc
    19741 ggcctacggt tgggcatcgt ttgagtcact gtcggtcgga taagatgcgc aagtatcgca
    19801 tccgacatta ttgcggcact ggagtttggc aacagtgccg gatgcggcgc gagcgcctta
    19861 tccggcctac ggttgggcat cgtttgagtc actgtaggtc ggataagatg cgcaagcatc
    19921 gcatccgaca ttattgcggc actggagttt gacaacagtg ccggatgcgg cgcgagcgcc
    19981 ttatccggcc tacgttttaa tgccagcaaa aatggtgaat tacctgggtt atcagttcgc
    20041 gggtgggctt gataaaccgt gtttccagat attcatcagg ctgatgagcc tgattaattg
    20101 agccaggccc cagaaccagc gtcgggcata acgtttgaat aaacggcgct tcggtacagt
    20161 agttcaccac ttcggtcttt gctccgagca atttctcaac cacttcaacc agttgatgat
    20221 tcggtgggca ttcatagcca gggatcggcg gatgcagctc gtcgaccgtc agacgacccg
    20281 gccagcgttc gctcaccgga gccaatgcat cgttgagcaa accattaagt tcattgagtg
    20341 tcatgccagg cagcggacga atatccatat gcaactcaca gcaagcgcaa atacggttag
    20401 aagcgtcgcc accgtgaata tgcccgaggt tgagcgtagg atatggcacg gtaaacgctt
    20461 cgtagtgata acgttctttc aggttatcgc gcaattgcaa aatatgcccg atggcgtcgt
    20521 gcattagttc gatagcgtta actccgcgtg ctggatcgct ggagtgcccc gactggccct
    20581 gaatacggat ggcgttagag atatgacctt tatgtgcgcg taccggttgt agtgacgtcg
    20641 gttcgccaat gatggcgcaa tccgggcgca gggcggtagt ttcggcaaaa taacgcgctc
    20701 cggccatact ggtttcttca tcagcagtcg ccagaatgta gagcggtttt ttcagtttcg
    20761 tgacgtcgac atcgcgtagc gcatcaagga taaacgcaaa aaagcctttc atgtcggcgg
    20821 tgcctaagcc gtaaagcttg ccgtcatgct ccgtcagtgt aaacggatcg cgcgtccagc
    20881 gaccgtcatc aaatggcacc gtatcggtat gccccgccag caacaagccg ccagccccct
    20941 gtccgcaact ggccagcata ttaaatttgt tgcgagttcc tggcacaggc tgcacttcca
    21001 cattgaagcc taaatcttta aaccagtccg ccagcagagt gattaaatct gcattgcttt
    21061 gatcgagtgc ctcttccgtg gcgcttattg aaggtgtggc aatcagagca cggtaaatct
    21121 cgataaatgg cggtaatttg tttttcattg ttgacacacc tctggtcatg atagtatcaa
    21181 tattcatgca gtatttatga ataaaaatac actaacgttg agcgtaataa aacccaccag
    21241 ccgtaaggtg aatgttttac gtttaacctg gcaaccagac ataagaaggt gaatagcccc
    21301 gatgttgaat acgctgattg tgggtgccag cggctacgct ggcgcagagc tagtgaccta
    21361 tgtaaatcgc catccgcata tgaacataac cgctttgact gtttcagcgc aaagcaatga
    21421 tgcgggaaag ttaatctccg atttgcatcc gcagctaaaa ggcatcgttg atctgccgtt
    21481 gcagccgatg tcggatatca gcgagtttag cccaggggtg gacgtggtgt ttctcgccac
    21541 cgcccatgaa gttagccacg atttagcgcc gcagtttctt gaagcgggct gcgtggtgtt
    21601 cgacctttcc ggcgcgtttc gtgttaacga cgccaccttc tatgaaaaat attacggctt
    21661 tacccatcaa tacccggaac tgctggaaca ggcagcctac ggtctggcgg agtggtgcgg
    21721 taataaatta aaagaagcga atttgattgc ggtgccaggc tgttatccga cggcagcaca
    21781 gttggcgctg aaaccgttga ttgatgccga tcttcttgac ctcaatcagt ggccggtgat
    21841 caacgccacc agcggcgtga gcggtgcagg acgtaaggca gcgatttcaa atagtttttg
    21901 tgaagttagc ctgcaaccgt atggcgtctt tactcatcgc catcaaccag agatcgccac
    21961 acacctcggt gctgacgtta tcttcacccc gcatctgggc aatttcccgc gcggcattct
    22021 cgaaaccatt acctgccgcc tgaaatcggg tgtgacccag gcgcaagtcg cgcaagtgtt
    22081 acagcaggcg tatgcccata aaccgctggt gcggctgtat gacaaaggcg ttccggcgct
    22141 gaaaaatgtc gttgggctgc cattttgcga tatcgggttt gccgttcagg gcgagcattt
    22201 gattattgtg gcgaccgaag acaacttact gaaaggcgcg gcggcacaag cggtacagtg
    22261 cgccaatatt cgtttcggct atgcggaaac gcagtctctt atttaagggt gcaatgatga
    22321 atccattaat tatcaaactg ggcggcgtac tgctggatag tgaagaggcg ctggaacgtc
    22381 tgtttagcgc actggtgaat tatcgtgagt cacatcagcg tccgctggtg attgtccacg
    22441 gcggcggttg cgtggtggat gagctgatga aagggctgaa tctgccggtg aaaaagaaaa
    22501 acggcctgcg ggtgacgcct gcggatcaga tagacattat caccggagca ctggcgggaa
    22561 cggcaaataa aaccctgttg gcatgggcga agaaacatca gattgcggcc gtaggtttgt
    22621 ttctcggtga cggcgacagt gtcaaagtga cccagcttga tgaagagtta ggtcatgttg
    22681 gactggcgca gccaggttcg cctaagctta tcaactcctt gctggagaac ggttatctgc
    22741 cggtggtcag ctccattggc gtaacagacg aagggcaact gatgaacgtc aatgccgacc
    22801 aggcggcaac ggcgctggcg gcaacgctgg gcgcggatct gattttgctc tccgacgtca
    22861 gtggcattct cgacggcaaa gggcaacgca ttgccgaaat gaccgccgcg aaagcagaac
    22921 aactgattga gcagggcatt attactgacg gcatgatagt gaaagtgaac gcggcgctgg
    22981 atgctgcgcg cacgttgggc cgtccggtag atatcgcctc ctggcgtcat gcggagcagc
    23041 ttccggcact gtttaacggt atgccgatgg gtacgcggat tttagcttaa gttttgttgg
    23101 ccggaggcgt agctttccgg cattgaattt caaaataagg aaacagagtt atggcacttt
    23161 ggggcgggcg ttttacccag gcagcagatc aacggttcaa acaattcaac gactcactgc
    23221 gctttgatta ccgtctggcg gagcaggata ttgttggctc tgtggcctgg tccaaagccc
    23281 tggtaacggt cggcgtgtta accgcagaag agcaggcgca actggaagag gcgctgaacg
    23341 tgctgctgga agatgttcgc gccaggccac aacaaatcct tgaaagcgac gccgaagata
    23401 tccatagctg ggtggaaggc aaactgatcg acaaagtggg ccagttaggc aaaaagctgc
    23461 ataccgggcg tagtcgtaat gatcaggtag cgactgacct gaaactgtgg tgcaaagata
    23521 ccgttagtga gttactgacg gctaaccggc agctgcaatc ggcgctggtg gaaaccgcac
    23581 aaaacaatca ggacgcggtg atgccgggtt acacccacat gcaacgtgct caaccggtga
    23641 cgttcgcaca ctggtgcctg gcttacgtgg agatgctggc gcgtgatgaa agccgcttgc
    23701 aggatgcgct taagcgtctg gatgtcagtc cgttaggctg tggcgcgctg gcgggaacgg
    23761 cttatgaaat cgaccgtgaa cagttagctg gctggctggg ctttgcttca gcgacccgta
    23821 acagtctcga cagcgtttct gaccgtgacc acgtgctgga actgctttct gctgccgcta
    23881 tcggcatggt gcatctgtcg cgttttgctg aagatctgat tttctttaac accggcgaag
    23941 cggggtttgt ggagctttct gaccgcgtga cttccggttc atcattaatg ccgcagaaga
    24001 aaaacccgga tgcgctggag ctgattcgcg gtaaatgcgg tcgggtgcag ggtgcgttaa
    24061 ccggcatgat gatgacgctg aaaggtttgc cgctggctta caacaaagat atgcaggaag
    24121 acaaagaagg tctgtttgac gcgctcgata cctggctgga ctgcctgcat atggcggcgc
    24181 tggtgctgga cggcattcag gtgaaacgtc cacgttgcca ggaagcggct cagcagggtt
    24241 acgccaacgc caccgaactg gcggattacc tggtggcgaa aggcgtacct ttccgcgagg
    24301 cgcaccatat tgttggtgaa gcggtggtgg aagccattcg tcagggcaaa ccgctggaag
    24361 aactgccgct cgacgagttg cagaaattca gcccggtgat tggcgaagat gtctatccga
    24421 ttttgtcgct gcaatcgtgc ctcgacaagc gtgcggcaaa aggcggcgtc tcactgcagc
    24481 aggtggcgca ggcgattgct tttgcaaggg cgcggttggg gtaagaacat ttatatgtac
    24541 aaatttgagc ctggcttatc gccgggcttt tttatggcaa aaaaaagcgg atcctggaga
    24601 tccgcaaaag ttcacgttgg ctttagttat tcgagttgag aaactctcga aacgggcagt
    24661 gacttcaagg gttaaaagag gtgccgctcc gtttctgtga gcaattatca gtcagaatgc
    24721 ttgataggga taatcgttca ttgctattct acctatcgcc atgaactatc gtggcgatgg
    24781 aggatggata atgaatattc gtgatcttga gtacctggtg gcattggctg aacaccgcca
    24841 ttttcggcgt gcggcagatt cctgccacgt tagccagccg acgcttagcg ggcaaattcg
    24901 taagctggaa gatgagctgg gcgtgatgtt gctggagcgg accagccgta aagtgttgtt
    24961 cacccaggcg ggaatgctgc tggtggatca ggcgcgtacc gtgctgcgtg aggtgaaagt
    25021 ccttaaagag atggcaagcc agcagggcga gacgatgtcc ggaccgctgc atattggttt
    25081 gattcccacg gttggaccgt acctgctacc gcatattatc ccgatgctgc accagacctt
    25141 tccaaagctg gaaatgtatc tgcatgaagc acagacccac cagttactgg cgcaactgga
    25201 cagcggcaaa ctcgattgcg tgatcctcgc gctggtgaaa gagagcgaag cattcattga
    25261 agtgccgttg tttgatgagc caatgttgct ggctatctat gaagaccacc cgtgggcgaa
    25321 ccgcgaatgc gtaccgatgg ccgatctggc tggggaaaaa ctgctgatgc tggaagatgg
    25381 tcactgtttg cgcgatcagg caatgggttt ctgttttgaa gccggggcgg atgaagatac
    25441 acacttccgc gcgaccagcc tggaaactct gcgcaacatg gtggcggcag gtagcgggat
    25501 cactttactg cctgcgctgg ctgtgccgcc ggagcgcaaa cgcgatgggg ttgtttatct
    25561 gccgtgtatt aagccggaac ctcgccgcac tattggcctg gtttatcgtc ctggctcacc
    25621 gctgcgcagc cgctatgagc agctggcaga ggccatccgc gcaagaatgg atggccattt
    25681 cgataaagtg ttaaaacagg cggtttaaac cgtttaacgc agctacccga taggcttccg
    25741 ccatcgtcgg gtagttaaag gtggtgttga cgaagtactc aatagtgttg ccgccacctt
    25801 tctgttccat aatcgcctga ccgatatgaa taatttcggc agcgcgctcg ccaaagcagt
    25861 gaatgcccag aatctctttt gtttcccgat ggaacaaaat tttcagcgtg cccacgttca
    25921 tgccgacgat ttgtgcacgt gccagatgtt tgaactgggc gcggcccact tcatatggca
    25981 ctttcatcgc ggtcagctgc tgttcggttt tgcccacaga gctgatttcc gggatggtgt
    26041 aaatgccggt agggatatct tcaatcagat gtgcggtggc ttcacctttc accagcgcct
    26101 gcgcggcaat gcgcccctgg tcataggccg ccgacgctag gctcggataa ccaatcacgt
    26161 cgcccaccgc gtaaacgtgc ggctgtgcgg tctgatacat gctgttgacc ttcagctgtc
    26221 cgcgactgtc agtttccagc ccaatgttct gtaacgccag cgaatcagta ttaccggtgc
    26281 gaccgttggc atagagcagg cagtcagctt tcagtttttt acccgacttc agatgcatga
    26341 tcacaccgtc gtcacagcct tcgatcttct cgtactcttc gttgtggcga atcactacgc
    26401 cactgttcca gaagtgatag gagagagaat ctgacatctc ttgatcgaga aatgccagca
    26461 gccgatcgcg ggtgttgatc agatccactt ttacatccat accgcggaag atcgacgcat
    26521 attcacagcc gatcactcca gcaccataga taagtacatg gcgcggttcg tggtgcatgc
    26581 taagaattga gtcgctgtcg taaatgcgtg gatgggtgaa atcaacatct gttggatgat
    26641 atggacgaga gccgcaggca ataacaaatt tttcagcggt tagtgtttca acgctgccgt
    26701 ccgggcaatc cagcgccaac gtatgctcgt caacaaagcg agcgttcccc tgcaatattt
    26761 cacagtgatt acgttcgtaa aatccctgac gcatgcgcgt ttgttgatta atcacgttat
    26821 cggcatggtt aaggatatcg gcaaaagaag agcggagcag tcgggaatgg tcgctgtata
    26881 gtgggttttg attgaattct ataatgcggc tgacggcgtg acggagagct ttcgacggga
    26941 tggtgcccca gtgggtgcaa ccgccgccaa cattttgata acgctcgata actgcgatgc
    27001 gcgcaccttg cttaaccagg cccattgcag cgccttcgcc gccggggccg gaacctatta
    27061 ctatggcatc gtaatcgtag gaatgtggca tggtagggct tacctgttct tatacataaa
    27121 agcaacagaa tggtaacatt ttatcgcggg taagccaatt gatccccgtc attaatctgg
    27181 ctatagcgtg agcggccttt gctttgtctg cctccttctt aacccattta ttcactatta
    27241 atcgcttttt cttatggctg atagttttat cttgccgaag cgtgcttttt tgttattgtc
    27301 tttatgaaat c
//
LOCUS       DAZUCE010000042        27299 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZUCE010000042 DAZUCE010000000
VERSION     DAZUCE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.042"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            117..659
                     /gene="zapC"
                     /locus_tag="ACHY03_001528"
     CDS             117..659
                     /gene="zapC"
                     /locus_tag="ACHY03_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFS3904775.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(656..1765)
                     /gene="ycbX"
                     /locus_tag="ACHY03_001529"
     CDS             complement(656..1765)
                     /gene="ycbX"
                     /locus_tag="ACHY03_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFS3904776.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            2009..4117
                     /gene="rlmKL"
                     /locus_tag="ACHY03_001530"
     CDS             2009..4117
                     /gene="rlmKL"
                     /locus_tag="ACHY03_001530"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFS3904777.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4129..6036
                     /gene="uup"
                     /locus_tag="ACHY03_001531"
     CDS             4129..6036
                     /gene="uup"
                     /locus_tag="ACHY03_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3904778.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6166..7419
                     /gene="pqiA"
                     /locus_tag="ACHY03_001532"
     CDS             6166..7419
                     /gene="pqiA"
                     /locus_tag="ACHY03_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFS3904779.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELSVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCGTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7424..9064
                     /gene="pqiB"
                     /locus_tag="ACHY03_001533"
     CDS             7424..9064
                     /gene="pqiB"
                     /locus_tag="ACHY03_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFS3904780.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9061..9624
                     /gene="pqiC"
                     /locus_tag="ACHY03_001534"
     CDS             9061..9624
                     /gene="pqiC"
                     /locus_tag="ACHY03_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFS3904781.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9880..10047
                     /gene="rmf"
                     /locus_tag="ACHY03_001535"
     CDS             9880..10047
                     /gene="rmf"
                     /locus_tag="ACHY03_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFS3904782.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10117..10635)
                     /gene="fabA"
                     /locus_tag="ACHY03_001536"
     CDS             complement(10117..10635)
                     /gene="fabA"
                     /locus_tag="ACHY03_001536"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFS3904783.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10704..12464)
                     /gene="ycbZ"
                     /locus_tag="ACHY03_001537"
     CDS             complement(10704..12464)
                     /gene="ycbZ"
                     /locus_tag="ACHY03_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3904784.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEEYEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIVSLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12650..13102
                     /gene="matP"
                     /locus_tag="ACHY03_001538"
     CDS             12650..13102
                     /gene="matP"
                     /locus_tag="ACHY03_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFS3904785.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13179..14231)
                     /gene="ompA"
                     /locus_tag="ACHY03_001539"
     CDS             complement(13179..14231)
                     /gene="ompA"
                     /locus_tag="ACHY03_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFS3904786.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(14588..15097)
                     /gene="sulA"
                     /locus_tag="ACHY03_001540"
     CDS             complement(14588..15097)
                     /gene="sulA"
                     /locus_tag="ACHY03_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFS3904787.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLPPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15316..15945
                     /gene="sxy"
                     /locus_tag="ACHY03_001541"
     CDS             15316..15945
                     /gene="sxy"
                     /locus_tag="ACHY03_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFS3904788.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     NGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15908..18070)
                     /gene="yccS"
                     /locus_tag="ACHY03_001542"
     CDS             complement(15908..18070)
                     /gene="yccS"
                     /locus_tag="ACHY03_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFS3904789.1"
                     /translation="MAYMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18080..18526)
                     /gene="yccF"
                     /locus_tag="ACHY03_001543"
     CDS             complement(18080..18526)
                     /gene="yccF"
                     /locus_tag="ACHY03_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFS3904790.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18649..20703
                     /gene="helD"
                     /locus_tag="ACHY03_001544"
     CDS             18649..20703
                     /gene="helD"
                     /locus_tag="ACHY03_001544"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFS3904791.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQHRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVLIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20735..21193)
                     /gene="mgsA"
                     /locus_tag="ACHY03_001545"
     CDS             complement(20735..21193)
                     /gene="mgsA"
                     /locus_tag="ACHY03_001545"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFS3904792.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21289..21951)
                     /gene="yccT"
                     /locus_tag="ACHY03_001546"
     CDS             complement(21289..21951)
                     /gene="yccT"
                     /locus_tag="ACHY03_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFS3904793.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22124..22537
                     /gene="yccU"
                     /locus_tag="ACHY03_001547"
     CDS             22124..22537
                     /gene="yccU"
                     /locus_tag="ACHY03_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFS3904794.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22582..22899)
                     /gene="hspQ"
                     /locus_tag="ACHY03_001548"
     CDS             complement(22582..22899)
                     /gene="hspQ"
                     /locus_tag="ACHY03_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFS3904795.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22957..24147)
                     /gene="rlmI"
                     /locus_tag="ACHY03_001549"
     CDS             complement(22957..24147)
                     /gene="rlmI"
                     /locus_tag="ACHY03_001549"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFS3904796.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCNQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24242..24520
                     /gene="yccX"
                     /locus_tag="ACHY03_001550"
     CDS             24242..24520
                     /gene="yccX"
                     /locus_tag="ACHY03_001550"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFS3904797.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24517..24846)
                     /gene="tusE"
                     /locus_tag="ACHY03_001551"
     CDS             complement(24517..24846)
                     /gene="tusE"
                     /locus_tag="ACHY03_001551"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFS3904798.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24937..25596)
                     /gene="yccA"
                     /locus_tag="ACHY03_001552"
     CDS             complement(24937..25596)
                     /gene="yccA"
                     /locus_tag="ACHY03_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFS3904799.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(26004..27023)
                     /locus_tag="ACHY03_001553"
     CDS             complement(26004..27023)
                     /locus_tag="ACHY03_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3904800.1"
                     /translation="MGRPRKNKKDNALPPRVRSNGYSYVWKPEGSTKTIGLGRVRETS
                     IAKVWQNYELEKAKRHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQRALLAVFGK
                     VLADNVKIEQVRIFMDKRGIESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFT
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSGTCEYVINTTKGGKVIAKTLNNWWNQAKHAA
                     EKKSGIPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26992..27243)
                     /locus_tag="ACHY03_001554"
     CDS             complement(26992..27243)
                     /locus_tag="ACHY03_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFS3904801.1"
                     /translation="MYELTLSPAEIQEITRYERYTKQQQQLRLHGIPFVPGPKNEPIV
                     LRSDVPRGLTAIPKVSELVSADPDFEALNNGKTKKKQKR"
BASE COUNT         6935 a         6645 c         7108 g         6611 t
ORIGIN      
        1 gttttatatt catcgctgtt gcttatttag acattttgta cttttattgt tgaggttatt
       61 aagcgaagcg acaatggatt gtgttgctgc ggttttatag ggtaggggag aggcagatgc
      121 gaattaaacc agacgataac tggcgttggt attacgatga agagcacgat cgaatgatgc
      181 tcgatttagc caatggtatg ctatttcgct cacgttttgc gcgcaagatg ttgactccag
      241 atgccttttc tcccgcaggc ttttgcgttg acgacgccgc gctctatttc tcttttgaag
      301 aaaagtgccg cgattttaat ttatctaaag aacaaaaagc cgaactggtg ctgaatgcac
      361 tggtagcaat tcgctatctc aaaccgcaaa tgccgaaaag ctggcatttt gtttcccatg
      421 gtgaaatgtg ggtgcccatg ccgggcgatg cggcctgcgt ttggttgagc gatactcacg
      481 agcaggtcaa tttgctggtc gttgaatctg gcgaaaatgc cgcactatgc ctgctggcac
      541 aaccctgcgt tgttattgcg ggtcgcgcga tgcagttggg cgacgccatt aaaatcatga
      601 acgacaggct gaaaccgcag gttaatgttg acagcttcag cctcgaacag gcagtctaac
      661 gcgccaactt aagtgcagtc ttcggtacac agctacagca aagaatggtg ccatcatcgc
      721 ccattgctga ttttttcagc ggcgtgactt cgccttctaa aagctgaaca cggcaacttc
      781 cacaaatgcc cgcgcggcaa gaataaggga tacgaattcc ctgattttct aattgctcca
      841 gcaacacctg ttggttattt ccacgaaatg cctgtccctg ccagtcaata tctacatttg
      901 cgtccggttg ttgcgtgatg ttggcggtat catcagcggc agctgcgccg taaatttttg
      961 ccggagccgt tgccagaatt tccacctcat cgccaacgcg aatcacgccg ctattacggg
     1021 caattaaatt ctgaccaaaa tcgacatcgc cgttatcctg ggcagtgcgg aaagattgca
     1081 atgtttttaa tggttcgcct gccggatgtt tttgcccttt ttctgggctg acggtggtga
     1141 aaatacagcg gctacaaggt ttaaccacat caaacaccac atcaccaatg cgaatcactt
     1201 tccagcgatc ttcttcccag gctgacgcgc cggaaaccac cagattgggg cggaattgct
     1261 ccatttttac actggccgga caacgttgtt ggagatcacg taacgaggct tcgttagcaa
     1321 gaaggtaagg atagccatca gcaaatgaca gaggtacagt gttgtggcgt ttcacgcgcc
     1381 gggtcatttg tggccccacc cagcgtaatt gcacttcgcg ggagaaaaat ccacttagcc
     1441 atttgttgat cgcgtctggc gcaattcgcg cggtaaaatg tgtgccccaa acttcggttg
     1501 gtgcgtcttg tgtggcgaaa tcagcaaaac gaacatatgc gctactgcca tctggtgcgg
     1561 tgagatgcaa gccatcatgc acaggtgaag gagtaaaccg taccatctgg ggaaactggc
     1621 gagcggtaat aaacgtaccg tcaggttccg tgatcataaa gatgcgatcg aaggccagac
     1681 cactgacatc agccagagca tgtgtaagac caatgccgcg catcgattta acaggatgaa
     1741 taaaaagccg ggttaatgtc gccacggtgc ggtcctcaaa tgaaaataag ccctcaactt
     1801 tatgacatga cgcgcttatt agctataatg cgcaacaatt ttcttagcgc ctgtcccatt
     1861 aggctatttt atttgtcagt ttggccctgg gttgtgctcg aaatcctcac gtacttgtgt
     1921 acgctccggt ttctccgcgc tgtccatgtc cagactgact gcaacaatta cgcctactgc
     1981 ggtaggctct tagagtaaaa gtgacgatat gaattctctg tttgccagta cggcccgtgg
     2041 gctggaagag ctgttaaaaa ctgaactgga aaacctgggg gccgttgaat gccaggtggt
     2101 tcagggtggg gtccatttca agggcgacac acggcttgtt taccagagcc tgatgtggag
     2161 ccgcctggcc tcgcgtatta tgttgccgct gggcgagtgt aaggtttaca gcgatttaga
     2221 cctctatctc ggtgttcagg cgatcaactg gacagagatg tttaatcctg gcgcgacctt
     2281 cgctgtccac ttcagtggtt tgaatgacac catccgcaac agtcagtacg gcgcgatgaa
     2341 agtgaaagac gcgatcgtcg atgctttcac gcggaaaaat ctgccgcgtc caaatgttga
     2401 tcgcgatgcg ccggatatcc gcgttaacgt ctggctgcat aaagaaaccg ccagtatcgc
     2461 tcttgatctg agtggtgatg gtttacatct gcgtggctat cgcgatcgtg ctggtattgc
     2521 gccgatcaaa gaaaccctgg cagccgctat tgtgatgcga tccggctggc agccaggaac
     2581 accgctgctc gatccgatgt gtggttccgg tacgttgctg attgaagcgg cgatgctggc
     2641 gaccgatcgc gcaccaggct tgcaccgtgg gcgttggggc tttagcggct gggcgcagca
     2701 tgatgaagct atctggcagg aagtgaaagc ggaagcgcaa actcgtgccc gtaaaggcct
     2761 ggctgagtat agctctcatt tttacggttc ggacagcgac gcacgggtga ttcaacgtgc
     2821 gcgcactaac gcccgtcttg cggggattgg tgaactgatc acctttgagg tgaaagatgt
     2881 cgcgcaactg accaatccgc tgccgaaagg gccgtacggt acagtgttga gcaacccgcc
     2941 atacggggaa cgtctggaca gcgaaccggc gctgattgcg ctgcatagtc tgctgggccg
     3001 aatcatgaaa aaccagttcg gtggctggaa tctctctttg tttagcgcct cgccggatct
     3061 gctcagctgc ttgcagctgc gtgcagacaa acagtacaag gcgaaaaacg gcccgctgga
     3121 ctgcgtacag aaaaattacc acgttgccga aagcactcca gacagcaaac cggcgatggt
     3181 agcggaagac tacgccaacc gcctgcgtaa gaacctcaaa aaattcgaga agtgggctcg
     3241 ccaggaagga attgaatgtt accgcctgta tgacgccgat ctaccggaat ataacgttgc
     3301 cgttgaccgt tatgccgact gggtggtggt gcaggagtat gcgccgccaa aaactattga
     3361 tgctcacaaa gcgcgtcagc gtctgttcga tattatcgct gcaaccattt cggtactggg
     3421 gattgcgcca aacaaactgg tgctgaaaac ccgtgaacgc cagaagggca aaaatcagta
     3481 ccagaaactg ggcgagaagg gcgagtttct tgaagttacc gaatataacg ctcacttgtg
     3541 ggtgaacctg acggattacc tcgataccgg tctgtttctc gatcaccgta tcgcccgtcg
     3601 tatgctcggt cagatgagca aaggcaaaga tttcctcaac ctgttctctt ataccggcag
     3661 cgccaccgtg catgcgggat taggcggtgc acgcagcacc accaccgtgg atatgtcgcg
     3721 tacttatctg gagtgggcag aacgcaacct gcgtttgaat ggcctgaccg ggcgtgcgca
     3781 tcgcctgatt caggccgatt gcctggcgtg gctgcgtgag gcaaatgaac agttcgatct
     3841 gatctttatc gatccgccaa ccttctctaa ctcaaaacga atggaagatg cgtttgatgt
     3901 tcagcgcgat catctggcgc tgatgaaaga tttgaaacgt ctgctgcgtg caggtgggac
     3961 gatcatgttc tcgaacaaca aacgtggctt ccgtatggat ctcgacggcc tggcaaaact
     4021 gggactgaaa gcacaagaaa ttacgcaaaa aacgctctcc caggatttcg cccgtaaccg
     4081 ccagatccac aactgctggc tgattaccgc agcctgaaag gaatagtaat gtcattaatc
     4141 agtatgcatg gcgcatggct gtcgttcagc gacgcgccgc ttctcgataa cgcagaactg
     4201 catatcgaag ataacgaacg tgtttgtctg gtgggccgca acggcgcagg caaatcgacg
     4261 ttaatgaaaa tcctcaaccg tgaacaaggg ctggatgacg gtcgcattat ttacgagcag
     4321 gatttgattg tagcgcgtct gcaacaggat ccgccgcgta acgttgaggg tagtgtttat
     4381 gatttcgttg ccgaaggcat tgaagaacaa gcggaatacc tgaaacgcta tcacgatatt
     4441 tcgcgcctgg tgatgaacga cccgagcgag aaaaatctca acgaactggc gaaggttcag
     4501 gaacagctgg atcaccacaa cctgtggcag ctggaaaacc gcatcaacga agtgctggcg
     4561 caactggggt tagatcctaa tgttgctctg tcgtcgcttt ccggcggctg gttgcgtaaa
     4621 gcggcattag gacgcgcgct ggtgagcaat ccgcgcgtat tgctgctcga cgaaccgact
     4681 aaccacctgg atatcgaaac catcgactgg ctggaagggt tcctcaaaac cttcaatggg
     4741 acgattattt tcatctccca cgaccgctcg tttatccgca atatggcgac gcgtattgtt
     4801 gatctcgatc gcggcaagct ggtgacctat ccagggaatt acgaccagta cctgctggaa
     4861 aaagaagaag ccctgcgcgt ggaagaacta caaaatgccg agttcgatcg caaactggcg
     4921 caggaagagg tgtggatccg ccaggggatc aaagcacgcc gtacccgtaa tgaaggccgc
     4981 gtacgcgccc tgaaagcaat gcgtcgcgag cgtggcgaac gtcgcgaagt gatgggtacc
     5041 gcaaagatgc aggtggaaga ggccagccgc tccggtaaga tcgttttcga aatggaagac
     5101 gtttgctacc aggttgacgg aaagcaactg gtgaaagatt tttctgccca ggttctacgt
     5161 ggcgacaaaa ttgccctgat tgggccgaat ggctgcggca aaaccacgct gctgaaactg
     5221 atgctcggtc agcttcaagc ggacagcggg cgtattcacg ttggcaccaa actggaagtg
     5281 gcttatttcg atcagcaccg cgcggaactg gatcccgata aaacggtgat ggataacctt
     5341 gccgaaggta agcaagaggt gatggttaac ggcaagccac gccacgtttt gggctatttg
     5401 caggacttcc tgttccatcc gaaacgggcg atgacgccag tacgtgcgct ttctggcggt
     5461 gagcggaacc gcttgctgct ggcgcgttta ttcctcaaac caagcaactt attgattctt
     5521 gacgaaccga ccaacgatct tgatgtcgaa acgctggaac tgctggaaga actgatcgac
     5581 agctaccagg gcacggtatt gctggtaagc cacgatcgtc agtttgtcga taacaccgtt
     5641 acagaatgct ggatcttcga aggcggcggt aaaattggtc gttatgtcgg cggttatcat
     5701 gatgcccgtg gtcagcaaga acagtatgtg gcgcttaaac aacctgcggt gaaaaaaaac
     5761 gaagaagccg ctgcgccaaa agcagaaact gtaaaacgca gcagtagcaa actaagctat
     5821 aaattgcagc gcgaactgga gcagctaccg caattgctcg aagatctgga ggcgaagctg
     5881 gaagccctac agacgcaggt ggcggatgct tccttcttca gtcagccgca tgagcagacg
     5941 caaaaagtgc tcgctgatat ggctgccgca gaacaggaac tggagcaagc ctttgaacgc
     6001 tgggagtatc ttgaagcgtt aaaaaatggt ggctgatcgc aaaatagtcg atttaaacag
     6061 agacagctga caacggtaga attgcctgat gaactgggtt tatcaggcat gaaaaccaac
     6121 actttgttag tcatctgtaa aatagcgcat cattaaggag taccaatgtg cgaacatcat
     6181 catgccgcga agcacatcct gtgctcgcag tgtgacatgc tggtggcgtt accgcgcctt
     6241 gagcatggtc agaaagcggc atgtccccgg tgtggcacaa cgttaaccgt ggcgtgggat
     6301 gcccctcggc agcgtcctac cgcctatgcg ttggctgcac tgttcatgct gttgctgtcc
     6361 aacttgtttc cttttgtgaa tatgaacgtt gcaggagtta ccagtgaaat tacattactg
     6421 gaaattcccg gcgtgctttt ttctgaggac tacgccagcc tcggcacctt tttcctgttg
     6481 tttgtgcaac tggttcccgc gttttgtctg ataaccattc tgttactggt gaatcgcgcg
     6541 gaattatcgg tccgtttaaa agagcaactg gcacgggtgc tttttcaact caaaacctgg
     6601 ggaatggcgg agattttcct cgcgggtgtg ctggtcagtt tcgttaaact gatggcttac
     6661 ggcagcattg gggtaggcag cagctttctc ccctggtgtt tattttgtgt cctgcaactg
     6721 cgcgcttttc agtgcgttga tcgtcgctgg ttatgggacg atatcgcccc gatgccagaa
     6781 ctgcgccagc cgctaaaacc aggcgtcacg gggatacgtc aggggctgcg ttcgtgctcc
     6841 tgttgtacgg caatccttcc tgctgatgaa cccgtgtgcc cgcgctgtgg tactaaaggg
     6901 tacgttcgac gtagaaacag cctgcaatgg acactcgcgc tgcttgttac gtccatcatg
     6961 ctgtatcttc cggctaatat tttgcccatc atggtgacgg atttattagg ctcgaagatg
     7021 ccatcgacga ttctcgctgg ggtcatcctg ttatggagcg aaggctctta tcccgtcgct
     7081 gcggtgatct ttctggccag tattatggtg ccaacgttaa agatgatcgc catcgcgtgg
     7141 ctgtgttggg atgccaaagg gcatggcaag cgcgacagtg aaagaatgca tttgatttat
     7201 gaagttgttg agtttgtagg ccgctggtcg atgattgacg ttttcgttat cgcggtgctc
     7261 tcggcgctgg tgcgtatggg aggtttaatg agtatttatc cggcaatggg tgcattaatg
     7321 tttgctttag tcgtcataat gacaatgttt tctgctatga cgtttgaccc gcgtttgtcg
     7381 tgggatcgtc aacctgaatc agagcatgag gagtcctgac agtatggaat ctaataatgg
     7441 ggaagccaaa atccagaaag tgaagaactg gtctcccgtg tggatatttc ctatcgtcac
     7501 ggcgctcatt ggggcctggg ttctttttta tcattacagc catcagggac cggaagtgac
     7561 cttgatcacc gcgaatgcgg aaggaattga aggtggcaaa accaccatta aaagccgtag
     7621 cgttgacgtc ggcgtggttg aaagcgccac actcgctgat gatttgacac acgttgaaat
     7681 caaagcgcgg ctgaattccg gtatggaaaa attgctgcat aaagataccg tcttttgggt
     7741 ggtgaaaccg cagattggtc gagaagggat tagcggtctg ggaaccctgc tgtctggggt
     7801 ttatatcgaa ctgcagccag gcgcgaaagg cagcaaaatg gataaatacg atttgctgga
     7861 ctcgccaccg ttggccccgc ctgatgcgaa aggtatccgt gtgattctcg atagcaaaaa
     7921 agccgggcag ctctcgccag gagatccggt gctgttccgt ggctatcggg taggttcggt
     7981 tgaaaccagc accttcgata cgcaaaaacg caatatcagt tatcaactgt tcatcaatgc
     8041 accttatgac cgactggtga ccagcaatgt tcgcttctgg aaagatagtg gcattgcggt
     8101 tgatctgacg tcagcgggaa tgcgtgtgga gatgggctca ttgacaacgc tgctgagtgg
     8161 cggtgtcagc tttgatgtgc cggaaggtct ggatttaggg cagccagtgg caccgaaaac
     8221 agctttcgtt ttgtatgatg atcagaagag cattcaggat tcgttgtaca ccgatcacat
     8281 tgattatctg atgttcttta aagattcggt acgcggtctg caaccgggag ctccggtaga
     8341 attccggggt attcgcctgg gtaccgtaag caaagtgcca ttctttgcgc cgaatatgcg
     8401 tcagacattt aacgatgatt accgtattcc ggtgctgatt cgtatcgagc cagagcggct
     8461 gaaaatgcag cttggcgaaa atgcggatgt tgttgagcac cttggcgaat tgttgaaacg
     8521 tggtttacgc ggatcgctga aaaccggaaa cctggtcact ggcgcactgt atgttgatct
     8581 cgatttctat ccaaatacgc ctgcaataac cggtattcgt gaatttaatg gttatcagat
     8641 tatcccgacc gttagcggcg gcctggcgca aatccagcaa cgactgatgg aagcgttgga
     8701 taagatcaac aaactgccat tgaatccgat gattgaacag gcaaccagta cgctttctga
     8761 aagtcagcgc acaatgaaaa acctgcaaac gacgctggat agcatgaaca agatcctcgc
     8821 cagccagtcg atgcagcagt tgccgacgga tatgcagtca acgttgcgtg aattgaatcg
     8881 cagcatgcag ggcttccagc ctggctccgc agcctacaac aagatggtgg cggatatgca
     8941 gcgccttgat caggtgttgc gagaactgca accggtgctg aaaacgctca atgagaagag
     9001 taacgcgctg gtatttgaag cgaaggacaa aaaagatcca gagccgaaga gggcgaaaca
     9061 atgaaaaagt ggctagtgac gattgcagca ctgtggctgg ccggatgcag ctccggcgaa
     9121 attaataaaa actattacca gttacctgtg gtgcagagcg gtacacaaag taccgccagc
     9181 cagggcaatc gcctgttatg ggtagagcag gtcgctgtac ctgactatct ggcggggaat
     9241 ggtgtggttt atcaaaccag tgatgtgaag tatgtgattg ccaacaacaa cttgtgggcc
     9301 agcccgttgg atcaacagtt gcgcaacacc ctggtagcca acctgagcac gcaactgccc
     9361 ggctgggtgg ttgcctccca gcctctggga agcgcccagg acacgctcaa tgttaccgta
     9421 acggagttta acggtcgcta tgatggcaag gtcattgtca gtggtgagtg gctgttgaac
     9481 caccagggac aactgatcaa acgtccgttc cgtctggaag gagtgcaaac tcaggatggt
     9541 tacgatgaga tggttaaagt gctggccggt gtctggagtc aggaagccgc ttctattgca
     9601 caagagataa agcgtctacc ttaattataa agatttgtaa atataaccgt ctccggtatg
     9661 ttgcctgagg cggttttttt gtttctaacg tgcggaaaaa tttgttcctc ttcacatttt
     9721 ttgtacaacc gacatgcccg tgtagctcac aaatatgaca gtggcgtgaa ttttgcgcat
     9781 tgacggcagt tatgattcgc ggtattgctt aactgtgatt gcacatttag taatcactgt
     9841 tttcttttcc accagaaacc agtatgaggg aaacgaggca tgaagagaca aaaacgagat
     9901 cgcctggaac gggcacatca acgtggttat caggccggca tcgccggacg ctcaaaagaa
     9961 atgtgtccct atcagacgct gaatcaaagg tcacaatggc tgggaggctg gcgagaagcc
    10021 atggcggaca gggtagtaat ggcctgattc tgtctcttta aaaagaaacc tccgcattgc
    10081 ggaggtttcg ccttttgata ctctgtctga tgataatcag aaggcagacg tatcctggaa
    10141 cagaccgact ttcaggtcgc tggcggtata gatcagacga ccatcaacca gcacttcgcc
    10201 atctgccaga cccataatca gacgacgatt aacaatgcgt ttaaagtgaa tacggtaggt
    10261 cacttttttc gctgtcggca gtacctgacc agtgaatttc acttcgccaa cgcccagcgc
    10321 gcggccttta ccttcgccgc ccagccagcc gaggtagaac cctaccagct gccacattgc
    10381 gtccaagccc aggcatcccg gcataaccgg atcgccaatg aagtggcatc cgaagaacca
    10441 cagatccgga ttgatatcca gttctgcttc aacataccct ttgtcgaagt taccacccgt
    10501 ttcggtcatt ttgaccacac ggtccatcat tagcatgttc ggtgctggca attgcgggcc
    10561 tttagcgcca aacagttcac cgcgaccaga ggcaagaagg tcttcttttg tataggattc
    10621 gcgtttatct accatgttct ctgtaagcct tattttattg aagcacgcag gatagctaac
    10681 acgtgtacgc tgaacaagtc cgatcagttc ggaataaacc agttcagcca acgtaatggc
    10741 catggaaaac ggtgacgtcc ttcctgttgc gatgcttgcg cgatacgttc ctggatggtt
    10801 tgcatcagcg tcgtttggcc ttcgccatcc cacaccagat ttaataataa cggcagtgcg
    10861 tcagtcacat cgtctactgc ccagatggtg aatttgcctt cttctaccgc tttcaccagt
    10921 tcactgtgaa gacttaaatg gcgaacgtta gctgtgggga taatgacacc ttgtttaccc
    10981 gttaactcac gttgctggca aatagcaaag aagccttcga ttttctcatt taaaccaccg
    11041 accggctggg cgcgaccgaa ctgatcgact gaacctgtga tagcgatact ctgattcacc
    11101 ggcacatcag cgagggcgct tatcagggcg cagagttcag ccatcgaggc actatctcca
    11161 tcaacttcac tgtatgactg ctcaaatgtc agcgatgctg agaaggggat ctgttgctca
    11221 agctgtagtt ccgacatcag gaacgcctgc atgatcatca ttcctttcgc atggatattg
    11281 ccgccaagct ccgctttgcg ttcgatgtcg gtgaattcac catcgccaat atgcacaacg
    11341 cagctaatgc gagaaggttc gccaaaagcg cgtggatgac ccggaaattc aatgaccgaa
    11401 agggcgttaa tttgcccgat gcgttcgcct tcggtttcaa tcaggatttg ctcctgaagg
    11461 atctcatcct gcatacgttc agcgaggaaa ccttcgcgcc attcacgctg ctgcagcatt
    11521 aagtttagct gctcgccgga gaaggtgtcg ccatcacaca gggaggcgac ctctttacac
    11581 tggcggagga tccactgcgg gctaagcgga agtgtttctt gttcaccggt gtagcgtgct
    11641 gcttcgcgga taagtaccgg ccaggcatcc gctcccgggg caggtaagtg attatgtctg
    11701 gcggtaaatg tcacccagcg acaccactgg cttactgact ccgcatcgac aatctgcaga
    11761 gtatcttcaa attcgctata aatagcctgc tctgaaagct ctggctccat ctcctggaaa
    11821 tcagccaatg attcgcgttc gcctaccaga atgactttca gcttcaatgg catcgaaggc
    11881 acagagacgg ggagagggcg cgactcatca aacgcaaccc agtcaaaacg ctcgcggtta
    11941 acgatatttt tcagccgcat ccacagcaga ggttgcgcca gcagtgtacg caaagagacg
    12001 atgagaatac cgccatttgc ctgatgcacc agaccaggct gcagggtaat gtcgccatta
    12061 aactggcgca ggcagccaaa gagttgctcc gcttctaccc agtcggcagc gacaacttgc
    12121 gttaaagtcg caaaattatc atctgcactc actgcgtgac gtaagcggat ggagtggccg
    12181 gaaacctcat agtgatcgcc caccagttgg cctgcatcgc tttgtagcgt acgcgcggca
    12241 tcggcaataa gattcagata ctcatactct tccggggcct tagccagcat aaaagaggag
    12301 gatgctcgcg tatgcagcaa ctgctccagc gcaaattgca gtcgcggttg agtatcactg
    12361 aataaaggat cgttttcgtc aatcaaatgt ggctgagcaa atatttcctg atagctatcg
    12421 gtatcaggaa ccaggtcacg ccatgcaagt ttcgtaatgg tcaaagttga tgttttttag
    12481 tctgttgtca aagccgcgat tataccgtaa ccggcactac agcacacgta gaaagcaccg
    12541 acaatactca tggcatgggc gttaaagctc acaggatgga gattcttttc ttcactggcc
    12601 taaaaagctg atattctgta aagagttaca cggtaacatt gagatcgcta tgaaatatca
    12661 acaacttgaa aatcttgaaa gcggttggaa atggaagtat ctggtgaaga agcatcgcga
    12721 aggggagtta attacccgtt acatagaagc cagtgccgcc caggaagccg tcgatgtgtt
    12781 gctctcgctc gaaaatgaac ccgtgctggt aaatggctgg attgacaagc atatgaatcc
    12841 ggaactggtc aatcgcatga agcaaaccat tcgggccaga cggaagcgcc attttaatgc
    12901 ggaacaccag catacgcgca aaaagtcgat cgatctggaa tttattgtct ggcaacgtct
    12961 ggctggtctt gcgcagcgtc gcggtaaaac gctttctgaa acgattgttc agctgattga
    13021 agatgcggaa aacaaagaga aatacgcgaa taaaatgtct tctttgaagc aggatctgca
    13081 ggcattgctg ggtaaggaat aactgacgaa actcagttca atttactaaa ggtgaaaaaa
    13141 aaaccccgca gcagcggggt tttttaccag acgagaactt aagcctgcgg ctgagttaca
    13201 acgtctttga tacctttaac ttcgatctct acgcgacgat ccggagccag gcagtcgatc
    13261 agtgcagcac gctgtttcac gttgtcgcag gtgttgccag taaccgggtt ggattcgccc
    13321 ataccacgtg cggagatctt gtctgccggg atacctttgg agatcaggta atcaacaaca
    13381 gactgagcac ggcgctcgga cagaccctgg ttgtaagcgt cagaaccgat gcggtcagtg
    13441 taacccagaa caactacgga accgtctttc ggatccaggt tgctcagctg gctgtacagc
    13501 tgatccagag cagcctgacc ttccggtttc agggttgctt tgttgaagtt gaacagaacg
    13561 tcagacttca gagtgaagtg cttggtctgt acttccggtg ccggagctgg agccggagca
    13621 actaccggag ctgcttcgcc ctgaccgaaa cggtaggaaa cacccaggct cagcagaccg
    13681 ttgtccggac gagtaccgat ggtgtttgcg tcaccgatgt tgttggtcca ctggtattcc
    13741 agacgggtag cgatttcagg agtgatcgcg tactcaacac cacctgcgaa aaccggagaa
    13801 acgccagtgt cgtggtcttt aaaggatgcg ccaccaggta cgttagcctt ggtgtctgca
    13861 cgccatacca taccacccag acgagtgtag atgtccagat cgtcagtgat tgggtaaccc
    13921 agtttagcgg tcagctgaac gccctgagct ttgtatgcgc cgttgatgtt gtcgcctttg
    13981 tacggcatac gacctaacca gtcgtaaccc atttcaaaac caacatacgg gttaacctgg
    14041 taaccaccaa aagcacctgc acccagttgg ttttcgtggg tcggaccatt gttaggaata
    14101 aaaccagtgt catggtactg ggaccagccc agtttagcac cagtgtacca ggtgttatct
    14161 ttcggagcgg cctgcgctac ggtagcgaaa ccagccagtg ccactgcaat cgcgatagct
    14221 gtctttttca ttttttgcgc ctcgttatca tccaaaatac gccatgaata tctccaacga
    14281 gataacacgg ttaaatcctt caccggggga tctgctcaat attaactcta ccgatatctt
    14341 cggcttatgc cgagcacccc tggcgatgta aagtctacaa cgtagttgaa aacttacaag
    14401 tgtgaactcc gtcaggcata tgaaaaaaaa gtcttgtata aggtacgttt aatctttttt
    14461 gtcagcgaca atttacagaa aaaaaacgcg gaaaccgctt cagacaagcc tccgcaagga
    14521 aaattagtca cgactgaaag cattggctgg gcgacaaaaa aagttccagg attaatccta
    14581 aatttactta atgatacaaa ttagagtgaa tttttagccc ggaaagttgt ctcgttgcgt
    14641 gagaggatgc gcttaccgga cgcataataa accccatcgc gttaccttca tttgccgcat
    14701 caacaagttc agcatgctct tcttcagtca aatcatctgc caaccaaccg atcaccacac
    14761 tgtaattgcc cgtgcgtaaa gcgcgaacca ttgactccac agtgtggcaa ggggggagct
    14821 ggctaatctg cattactttc gttaagggta gcccagatgc ctgaacccat tcccgactca
    14881 gtttttgttg cggtgttaac cagagttgcc agcgcgattg ctgaccgagt tgctgtaaca
    14941 atggcaacag tagaagttgc gtcatcatgg gctgatcttc gcgatagaca acttcactga
    15001 taagcccggc tgtagtgttt tccgtagaga cacgcgcaat tttacttgct gcggatgaga
    15061 acgacgaaga acgatgtgca tagcctgaag tgtacataat caatccagcc cctgtgagtt
    15121 actgtatgga tgtacagtac atccagtgac aacaaagatc aaccctattt tcggaaagag
    15181 cctcgcaaat tttgtcgtta gtgacgggaa aacataaatt aatcttgccc cttaagaata
    15241 agttgcctat tttcgtattt aacggatccg ttaatgtgaa tcattctttt atgttatgat
    15301 tttaaaagga attttatgaa aagcctctcc tataagcgga tctataaatc acaagaatac
    15361 ctggcaacgt tgggcacaat tgaataccga tcattgtttg gtagttacag cctgaccgtt
    15421 gacgacacgg tatttgcgat ggtttctaat ggtgagttgt atcttcgggc ttgtgagcaa
    15481 agtgcacagt actgtgtaaa acatccgcct gtctggctga catataaaaa gtgtggccga
    15541 tccgttaccc tcaattacta tcgggttgat gaaagtctat ggcgaaatca actgaagctg
    15601 gtgcgtctgt cgaaatattc tctcgatgca gcgctgaaag agaaaagcac gcgcaatacc
    15661 cgggaaagac tgaaagattt gcccaatatg tcttttcatc tggaagcgat tctcggggag
    15721 gtggggatta aggatgtacg ggcgttacgt atacttgggg caaaaatgtg ttggttgcga
    15781 ctgcggcagc aaaacagtct ggtgacagaa aagattctgt ttatgcttga aggtgccatt
    15841 atcggcattc atgaagctgc gctcccggtg gcacgccgcc aggagcttgc agaatgggct
    15901 gactctctta cgccgaaaca ggagtttcct gcggaacttg agtaatctgg cgttgcagac
    15961 gaccaatctc aggcagtaat gcaatcaata acccaacttg ttgtacgacc agaggttctt
    16021 tgctgtctgc ccgtggttca agttgctgca tccgctgttt caggccagct aatgcctgat
    16081 tgacgcgttc ttcatcagca ggttgatgat gtaacgcgtc atcaacatag caaactgcgt
    16141 catcaagaaa cgccagaatt tcaggattag ttaactgctc ccggtgagca ccgagggctg
    16201 agatatagct ggtaaacgta tggttaaggc acagcaaccg aaacgcggct tcgcgaattt
    16261 gcggggtaac gttcggttcg ctggacatat ttgataccac cgacgccagt tcagcatcac
    16321 ggttgtgtgc atcgcggcgg gcaatacgat acgccagacg gttatcacgc ccctgatggt
    16381 attgctccag tatggcatcg agatagcgac agttggcctc tgtggcgcgt tcgagcatgc
    16441 gcggcagatt gcgaaactgc cagtcaggcc agatgtagct cactgccgcc cacgcaatgg
    16501 cacaaccaat cagcgtatcg attacgcgag gtaacgctac ttcaaaacct tcacccagta
    16561 agttaaaaca cagtagcacc aaaagtgtga tgaacatcgt tgcatgagcg tattgcacgt
    16621 tacggaaggc aaaaaatagc acgccggtaa taaccagcag caccagctgc ccttccagtg
    16681 atggcacaaa ccacagcaca ggaatgccaa tggcgatacc taccagcgta ccaataatcc
    16741 ttaacttcag gcggtggcgc gtggcgttat agtttggctg gcagacaaac aaacttgtca
    16801 gcaagatcca atacccgtga tgcattccgg ttatttgaat gatggcgtag ccgaagcaca
    16861 acaccagcga cattcttacc gcatgacgga agagggcgga ttccggcgtg aagtgacggc
    16921 taagacgcag ccagatatca ctcaatccgt gcgggctgtc atcagcgagc tcattttcgt
    16981 cattattatg gggcagtgcc tgggcctgtt ctgattcaat tgttgccagt tgggcatcaa
    17041 tggcgcgtaa attgttcagc aaaaatccca gtgttttgag taaatcggca ggcgcgccgt
    17101 tatcgcgcat ccgctccagg gcagcatcaa tatgcgtaaa agcgcgctca aaatgcggat
    17161 catgttgata aggctgacgc agcaaaatac agcgtgacag ttgctggcac gcctggccct
    17221 gcatcgacat cagccgctga aaacggaaca gcacgtcgct gtggcgaaaa tgttcacgca
    17281 atgtttgata ctgaatatga gaagagctgg cacgctcgtg aatatcctgt gcgacaaagt
    17341 aataatgcag cgtgcgacgc gttccccgtt gaccacgatc gccacgtaag cgggtcagca
    17401 gcgagagttt cgtctgattc aatgtcgcca tcagctgacc gttggcgaga gccaaatcgt
    17461 acagcggtgc ctggctttca tcttcaatat caggatcaaa catgcgcgac ttgagctcaa
    17521 gataacgcgc cagttgttca tagcaacgcg ccaggttgtc ctgcagcggg cggaccggga
    17581 acagcagatg accaataagt gtcaggacgt tgtaccagac ggcaccggcc agcagataca
    17641 tcggctgctg ataccagtgc tcatacagtg atgttcccaa cattgtgtaa atggcgatca
    17701 gcaatgcacc gaaggcaatt gttgcatagc gttgacccag accgccgagc aaaatgaagc
    17761 cgctggtaga gagcgttaag ccaatcgcaa atagccaggg ccagggaaac agcaattcta
    17821 ctgaggccga ggcgataaaa aagcagaaca gcgtaatgat gaggttacgc aaacgtcccg
    17881 ccagtcggtc atcgagatcg gtcagcgccg ctgccaccat ccccagcgtc agcggaatcg
    17941 tcagttttac atcacccagc caccacggaa acgctgtggt tccacaaagc gcaataaaaa
    18001 tacgcgcgta atacagccag gcgctgttcc aggtatagcg tttgagcaaa ggacttagca
    18061 tataggccat attagtggat tattcaaaac gacgacgtgc attggcttca cgcgcagctt
    18121 gcgctgtttc taccgatacc acgcgacgac caaccggcca tagtgcaata gcggcaattt
    18181 taaagttcgc aataccgaca ggaatgccaa tgatggaaat acattgtgcg atgcccgttg
    18241 caatgtgcat caggcataac caccagccaa agaaaatcag ccagaaaata ttcaataccg
    18301 taccgccagt attcagcagc acatttttgc cagccgggtt cagttcatcg acatgaatag
    18361 cttcattgcc ataaggcacc agagacagtt tagtgatctc ccagcaggat cgtgtcagcg
    18421 gtaaggtaaa aatcagcaca atactgacca gagtcgccaa cagccagccc agagtggtgg
    18481 caaatccgcc aagcacaaag ttcagaatgt tcaaaacggt acgcataaaa cctcgcttta
    18541 ctgtggtttt cagtaatggg cggcaattgt aacgtttttt tgggctggag cacgttttct
    18601 ctgacggtta cactgataag aaataatttc gtgtggatct acagagtcat ggaactgaaa
    18661 gcgacaacgc ttggaaaacg tctggcacag cacccttacg atcgggcggt gatcctcaat
    18721 gccgggatta aagtctccgg cgatcgccac gaatacctta ttcctttcaa tcaattactg
    18781 gcgattcact gtaagcgcgg tctggtatgg ggcgagctgg agtttgtact gccggacgaa
    18841 aaagtggtgc gtctgcacgg caccgaatgg ggcgagacgc agcgttttta ccatcatctt
    18901 gatgctcact ggcggcggtg gagtggagag atgagcgaaa ttgcgtctgg tgttttacgc
    18961 cagcaactgg atttgattgc cacgcgcact ggagaaaata aatggctgac gcgtgagcaa
    19021 acctctggcg ttcagcaaca aatccgccag gctttgtcgg cgttgccgtt gccggttaat
    19081 cgactggaag aattcgataa ctgccgtgag gcgtggcgta aatgtcaggc ctggttgaaa
    19141 gatattgaaa gcgctcggtt gcagcataac caggcgtata ccgaagccat gcttaccgag
    19201 tatgcggatt ttttccgcca ggtcgagtct tcaccgctga atccggcgca ggcccgggca
    19261 gtcgttaatg gcgagcattc tctgttagtg ctggcaggtg caggaagcgg aaaaacgtcg
    19321 gtgctggtgg cccgtgcagg ctggttgctg gcgcgtggtg aagcgtcccc tgagcaaatt
    19381 ttattactgg cgtttggtcg caaagccgct gaagagatgg acgagcgtat tcgcgaacgg
    19441 ctacataccg aagacattac cgcacgcacg tttcatgcgc tggcgctgca tattattcag
    19501 cagggcagca aaaaagttcc gatagtcagc aaactggaaa atgataccgc tgcccgtcat
    19561 gaactcttta ttgctgagtg gcgcaagcaa tgcagcgaaa agaaagcgca ggcgaagggc
    19621 tggcggcaat ggctgacgga agaaatgcag tggtcagtgc cagaaggtaa cttctgggat
    19681 gatgaaaaat tacagcatcg tcttgcctca cgcctcgatc gttgggtaag tctgatgcgg
    19741 atgcacggtg gtgcacaggc agaaatgatt gccagtgcac ccgaagagat tcgcgatctg
    19801 ttcagtaaac gtatcaagtt gatggctcca ttattaaaag cctggaaagg tgcgctgaag
    19861 gcagaaaacg ctgtcgattt ttcgggcctt attcaccagg cgattgtgat tctggagaaa
    19921 ggtcgcttta tcagcccgtg gaagcatatt ctggttgatg aatttcagga tatctcgccg
    19981 cagcgggcag cgttgttagc ggcattacgc aaacaaaaca gtcagacgac gttgttcgcc
    20041 gttggtgatg actggcaggc gatttaccga ttcagcggtg cgcaaatgtc gctcaccacc
    20101 gctttccatg aaaactttgg tgaaggagat cgctgtgatt tagacacgac ttaccgtttt
    20161 aacagtcgta tcggtgaggt ggcaaaccgg tttattcagc agaacccagg ccagctgaaa
    20221 aagccgctaa acagcttaac caatggagac aaaaaagccg tcacgttatt ggatgagagt
    20281 caacttgacg ctttgctgga taagctctct ggttatgcca aaccggaaga gcgcattctg
    20341 atcctggcgc gttaccatca catgaggcct gccagcctgg aaaaagcggc aacacgctgg
    20401 ccgaagttgc aaatcgactt tatgaccatt catgccagca aagggcaaca ggcggattac
    20461 gtcctcatcg ttggcttgca ggagggaagt gatggttttc cggctgcggc gcgggagtcg
    20521 attatggaag aggcgctact gccaccggtt gaggatttcc cggacgctga agaacggcgg
    20581 ttaatgtacg tggcgctgac ccgggcacgc catcgggtat gggcactgtt taacaaagag
    20641 aatccctctc cctttgtgga aatactgaaa aatctggatg tgccggtggc gagaaaaccg
    20701 taagaaacag gtggcgtttg ccacctgtgc aatattactt cagacggtcc gcgagataac
    20761 gctgataatc ggggatcaga atatcgaccg cgtcgttgaa atgcggcgac tggataatga
    20821 agtctgccgt tgccacgttg gtggcgaccg gaatgttcca taccgtcgcc agacgcagca
    20881 aggctttcac gtcaggatcg tgcggcacgg catttagtgg atcccagaag aaaatcaata
    20941 catcaatttt cccttctgag atcaatgcgc caacctgctg gtcacccccc attgggccac
    21001 tcaacatcgc gttgacgttc atgccggtcg cgcgggaaat taagttaccg gtagtgcctg
    21061 ttgcatacag tacgtgttgt tccagtaacg gttgatgccg ttccacccag ctcatcagca
    21121 tttgtttgca gtgatcgtgt gccaccagcg caatatgttt ccgcgcaggt aaagtgcgag
    21181 tcgtcagttc cataatgtac atccgtagtt aactttccta aagattactg taagcactta
    21241 tcgctgcaag ataaagaccg aaaaagcctg cgcacaggca caaaaatctc aggaagatgg
    21301 ttgtttttcc gcccattgca ggaaagtatt tcgcgtttgt gggtcagcca gtttaaacca
    21361 atacttcagc cgttgttctg tgagcacctg agactgcggt ggaatagcat ccagctcgga
    21421 aacacccgaa agcagcgtac tgtcatctgc catcatcgtg gcaaattgcg gtagtgaagc
    21481 gcgtttcgcg gatttgttat agcgttcaac ctcgacctca taatcaatcg tttttgccgt
    21541 tgaggtaatg gcgagaatat ccagctttac cggaatcggc gtcgcatcgc catccaacaa
    21601 ttcaaggcgc ggcgcggcat caaaatggtt agcttcccgc tcattctcca ggcgaggcag
    21661 gcgaaaattg acctggttga tgagctgggt attaaaactg accaccagcg gtggggagat
    21721 atacagccgt tcttcactat tggaaagatg aatcgtcttc tcaacgcgaa acactaactg
    21781 atgcggtcca ttatccagtt caatgctgtc cgcaccgcgc agcagagagc tggaaacctt
    21841 cttgccgtcg agtaccagaa gatcgacatc ggttgaaagc cgcagcgtag tggcaaaaac
    21901 ggataccggc aaacataatg caatcaaggt ggtcacgatg ccggttttca taggtagctc
    21961 ctgtcaaaag accgcccgaa taataatgtg tcaaaatttt tctttaatca tacttactaa
    22021 catatagaca tatttcccgc ttttgctctc attcattcgt attagctgca tggttggcat
    22081 gtcggctttg tcgtacactt tgttaaacag tctggaggaa ataatgaaag aaaccgatat
    22141 tgcaggcatt ttaacgtcca ctcacaccat tgcgctggtg ggggcaagcg acaaacccga
    22201 tcgtcccagc tatcgcgtga tgaaatatct actcgaccag gggtatcacg tcattccggt
    22261 ttcgccaaaa gttgccggca aaacgctgct gggccagcag gggtacggca cgctggcaga
    22321 cgttccggaa aaagtcgata tggtcgatgt tttccgtaat tcagaagcgg cgtggggcgt
    22381 agcgcaggaa gctatcgcca ttggcgcgaa aaccctctgg atgcaactgg gggtgattaa
    22441 tgagcaagcg gcagtactgg cacgggatgc cgggttgaat gtggtgatgg atcgctgccc
    22501 ggctattgag atccctcgcc tgggcctggc caaataaaaa atccccggaa ggcaaaaacc
    22561 ttccggggat ttgttcaggg attagttacg cagacgcggg gcctggagtt gtttgcggat
    22621 ggtctgcgcc agctcatcca tggatggctg ttcaggatgt tcatcctgca attcgctact
    22681 tagctgggct tcagccaggt aggtatgaac cggtaggccg ttatcgtcct ccatcaccac
    22741 gtgataccac ggagcggcgc gaagctcgtc attcaccgcc agctcatcag gcgacggttc
    22801 agagagcgaa taaaccgggt cgatatccac gaccactccg agataaccta acagggaatg
    22861 gcggacctgc tggccgatac cgaatttgct ggcaatcata gtcacctccc gggaaatctt
    22921 tacctttgat ataagggtaa aattccacat ttcaagttac atgacgcgac aggcaaaccc
    22981 tttcagatat agcccttccg gataggtagc gatcaccgga tgatcggccg cctgacggaa
    23041 ctgctctata aattgtacat cacgaccggc atcaattgcg gcatccgcga tgattttctg
    23101 aaataaatcg ctggtcatca gaccggaaca ggagaaagtc aggagaatac cgccttcatt
    23161 cagcaactga atcgccagca tgttgatgtc tttatagcca cgacacgcgc ccatcaactg
    23221 gcttttattc tcaacaaact tcggcgggtc catcacgata acgtcaaatt tttcaccgcg
    23281 atcgcgataa gtacgcagca atttaaagac atcatcacgg acaaactcag ccttgctcag
    23341 atccagtttg ttcagctcaa cgttctgccg tgcaatatcc agcgcttcct gggaggtatc
    23401 aacgctgaca acctggttgc aaccgcccat cagtgccgat acggcgaaac caccggtata
    23461 ggagaaacag ttcagtacgc gtttattttc aacgtagcgg cgggtagcca ggcggctgtc
    23521 tcgctggtcc aggtagtagc ccgttttgtg tccgtgctgt atgtccacca gcagcttcat
    23581 gccatgttct tcaatcggca gcagggcagg cggcaactcg ccggtgacgg ggccctgggt
    23641 cagctccatc ccttcttttt tacgtaccgc gacgtcgctg cgatcgtaaa tcgcacattc
    23701 tgggtacagc gtttgcaggg cactaattaa tgccgcgcgc tgatattctg cgccagcact
    23761 cagcagttgc agcaccagaa aattaccgaa acgatcgata gtaatacccg gcaggccatc
    23821 agattcaccg gcgattaaac gatagctgtc gaggccatct ttttgcgcca gccagtcacg
    23881 ccatttttgt gcttgttgca aacggcggga aaaaaaagca atgtcgatag actcagacgg
    23941 gtcaaacgtc cagacgcgcg cccggatttg cgaagctggc gaataagcgc cgcgtgctaa
    24001 ccattttccc tgatgatcaa caatatcgat ggtttcaccg aggctggctt taccttccat
    24061 gcgggcaacg gccccggaaa agacccacgg atggcgacga agtaatgatt tttcgcgccc
    24121 tttggctaac actaaacgta cactcataat ttacttttct gtcgatgcaa agaaatgggc
    24181 gtcattgtcc ggagttttcc atggatttgc aacgcgtcca ttaaggataa ggagagcagc
    24241 aatgtcgaaa gtctgcataa ttgcctgggt ttacgggcga gttcagggcg taggatttcg
    24301 ctacaccaca cagtacgaag cgaaaagact gggtttaacc gggtacgcca aaaatcttga
    24361 cgatggcagc gtggaagtgg ttgcctgcgg tgaagaaggg caggtagaaa aattaatgca
    24421 gtggttaaag agtggcggcc cgcgttctgc gcgtgtagaa cgggtgctta gcgagccgca
    24481 tcatccctcg ggggaattaa ccgattttcg tatccgttaa atacatttta ccggttttgg
    24541 caggccagca attttggtgg cttgctttgc cgggcctttc gggaacagtc ggtataaata
    24601 gcggctgtta cctttttctt caccaaattt attcgccatc gcttttacca gcatacgaat
    24661 cgccggagac gtattaaatt ccaggtagaa gtcacgcaca aaacgcacca cttcccagtg
    24721 ttctggcgac agcgaaatcc cttcgttctc tgcaatcacc actgccagcg gctcactcca
    24781 ctggctgctt tctttgagat agccttcggt atccgtttct atctctttac cttcgaagat
    24841 cagcataatt attactacct taatcagact gccggcagtg taacaaaaaa caaagccccg
    24901 cataaagcga ggctatgaaa gtgttagcgg gtgagattaa tcgcggctgg cgaagcccag
    24961 aatgctcagc aggctgacga agatgttgta cagcgaaaca tacaggctaa ccgtggcacg
    25021 aatatagttc gtttcaccgc catgaatgat gttgctggtt tcaaacaaaa tagcgccaga
    25081 ggagatcaaa atgaagaccg cgctgatcgc cagatgcaga gcaggcagtt gcaggaagat
    25141 attagcaacc ataccaatca gcaccaccac aatacccgcc atcagcatac cgccgaggaa
    25201 cgacatatct ttgcgggtgg tcagcacata tgcagagcag cagaagaaca ctaacgccgt
    25261 tccgcccagt gccatagcga ttacgtcacc cattccggca gacagatagg tgttcagaat
    25321 tggtccgagg atataaccca gaaaaccggt aaaggcgaat gcggagataa tcccggtcgg
    25381 cttattcgcc gttttatagg tcaggaacat caaaccatac atacccacca gcgtcagaat
    25441 cagacccgga gatggcagca tcagcaccgt actggcagtt gcggtaatcg ccgaaaaggc
    25501 cagcgtcagg ctcagcagaa aataggtatt acgcagcacc ttatgggtgc taagcagtga
    25561 tgtacggtca tgtgaagaac taacaatacg atccattgag tcactctcta tgacagatgt
    25621 aattaattaa gcagcataat gataatgcgt aaaggcaccc agaagtttta cccatcttta
    25681 cgcatttgat ctggaacagg tttaacagcg gattatcagg tcattaagca aatataacgc
    25741 cctgagaatt tcgacaggca aaagaaaaag gggttagcat ttagctaacc ccttatctta
    25801 tctggcggaa gcgcagagat tcgaactctg gaaccctttc gggtcgccgg ttttcaagac
    25861 cgatgaaaaa taaataaaaa tcaatgcaat actaaaagta gctgaaaaat taaccaaact
    25921 taacacctag aaaaatcagg ctgttaggga gctttcagag ttagtatacc aagtcttttt
    25981 tgccagaaaa tttcctgagt tgcctacttg ctgaccacaa gtggcaaatc cagtgtcggc
    26041 gtgatttttg ttttacgatc ataaatcaac acctgatttt ctgttttatg tccactgaaa
    26101 atctgtttgt cgcgactgct tccttcgtaa tctgatattc ctttggcttt tatgtcatgg
    26161 aaattgcacc caaacgggat gccggatttt ttctcggctg cgtgtttagc ctggttccac
    26221 cagttgttta gcgttttagc tatgaccttc ccgcccttgg ttgtgttgat cacatattca
    26281 catgtgcctg aagatacatt tctggctaac tggatcgcgg ttcgtaatcg tggagaccat
    26341 tccttgattt gtttggtgcc ggtcttgttt tgctcaatgt agattccttt atccataata
    26401 tcctgccatt tcagctcgag cacatcaccg agtcttgccg cacaaagata ggatatctcc
    26461 attgcaatgc gtaactgtgg tattgcttcc gcatatatcg ccgcatactg ttcatctgtg
    26521 atgtaaacag tgcgggcttt aagcgtaaat ttcctgactc ctttgcatgg gttattcttc
    26581 acatatccac gctcatatcc ccacccgtat acacgactga gacttgccag ttcatggttt
    26641 gcctgggtct tgctctcaat cccccgttta tccatgaaaa ttcttacctg ctcaattttt
    26701 acattatcag ccagtacttt tccgaagact gccagtaacg ccctctgatg ttgccgataa
    26761 tctttttggg ttctgggggc cagttctgta aatgcggggg aatccataaa catgtgccat
    26821 aatttagcga cggtcattat gttgtggcgt tttgcttttt ccagttcata attctgccag
    26881 actttagcta tgctggtttc ccgcactctt cctagcccta tagtttttgt gcttccttcg
    26941 ggtttccata cgtaactgta accattcgat ctaacacgtg gcggtagtgc attatctttt
    27001 ttgttttttc ttggtcttcc cattgttcag cgcctcaaaa tcggggtcag cagaaaccag
    27061 ttcagatact ttcggtatcg cagtcagtcc gcgcgggaca tcactgcgaa gaactattgg
    27121 ttcgttttta ggaccgggta caaatgggat accgtgcaac cttaactgtt gttgctgttt
    27181 tgtataccgt tcgtatctcg tgatctcttg aatttctgct ggcgatagag ttaattcgta
    27241 catgtggtca cgtgccttac agcatgaccg ccgccactat agcttgtggg cggcgatat
//
LOCUS       DAZUCE010000043        27147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZUCE010000043 DAZUCE010000000
VERSION     DAZUCE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.043"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(220..867)
                     /gene="ychE"
                     /locus_tag="ACHY03_001555"
     CDS             complement(220..867)
                     /gene="ychE"
                     /locus_tag="ACHY03_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFS3904802.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(898..951)
                     /gene="ychE"
                     /locus_tag="ACHY03_001556"
     CDS             complement(898..951)
                     /gene="ychE"
                     /locus_tag="ACHY03_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFS3904803.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1344..4019
                     /gene="adhE"
                     /locus_tag="ACHY03_001557"
                     /gene_synonym="adhC"
     CDS             1344..4019
                     /gene="adhE"
                     /locus_tag="ACHY03_001557"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFS3904804.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4320..4937)
                     /gene="tdk"
                     /locus_tag="ACHY03_001558"
     CDS             complement(4320..4937)
                     /gene="tdk"
                     /locus_tag="ACHY03_001558"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFS3904805.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5541..5954
                     /gene="hns"
                     /locus_tag="ACHY03_001559"
     CDS             5541..5954
                     /gene="hns"
                     /locus_tag="ACHY03_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFS3904806.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6098..7006)
                     /gene="galU"
                     /locus_tag="ACHY03_001560"
     CDS             complement(6098..7006)
                     /gene="galU"
                     /locus_tag="ACHY03_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFS3904807.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7208..8221)
                     /gene="rssB"
                     /locus_tag="ACHY03_001561"
     CDS             complement(7208..8221)
                     /gene="rssB"
                     /locus_tag="ACHY03_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFS3904808.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8313..9218)
                     /gene="rssA"
                     /locus_tag="ACHY03_001562"
     CDS             complement(8313..9218)
                     /gene="rssA"
                     /locus_tag="ACHY03_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFS3904809.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9331..9789
                     /gene="ychJ"
                     /locus_tag="ACHY03_001563"
     CDS             9331..9789
                     /gene="ychJ"
                     /locus_tag="ACHY03_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFS3904810.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9839..10681
                     /gene="purU"
                     /locus_tag="ACHY03_001564"
     CDS             9839..10681
                     /gene="purU"
                     /locus_tag="ACHY03_001564"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFS3904811.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVSRFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10841..10925
                     /locus_tag="ACHY03_001565"
     tRNA            10841..10925
                     /locus_tag="ACHY03_001565"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10875..10877,aa:Tyr,seq:gta)
     gene            10972..11103
                     /locus_tag="ACHY03_001566"
     ncRNA           10972..11103
                     /ncRNA_class="other"
                     /locus_tag="ACHY03_001566"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11287..11964)
                     /gene="narI"
                     /locus_tag="ACHY03_001567"
     CDS             complement(11287..11964)
                     /gene="narI"
                     /locus_tag="ACHY03_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFS3904812.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(11964..12674)
                     /gene="narJ"
                     /locus_tag="ACHY03_001568"
     CDS             complement(11964..12674)
                     /gene="narJ"
                     /locus_tag="ACHY03_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFS3904813.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LMKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(12671..14209)
                     /gene="narH"
                     /locus_tag="ACHY03_001569"
     CDS             complement(12671..14209)
                     /gene="narH"
                     /locus_tag="ACHY03_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFS3904814.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14206..17949)
                     /gene="narG"
                     /locus_tag="ACHY03_001570"
     CDS             complement(14206..17949)
                     /gene="narG"
                     /locus_tag="ACHY03_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFS3904815.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLKLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(18342..19733)
                     /gene="narK"
                     /locus_tag="ACHY03_001571"
     CDS             complement(18342..19733)
                     /gene="narK"
                     /locus_tag="ACHY03_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFS3904816.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            20072..21868
                     /gene="narX"
                     /locus_tag="ACHY03_001572"
     CDS             20072..21868
                     /gene="narX"
                     /locus_tag="ACHY03_001572"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFS3904817.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSAGVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            21861..22511
                     /gene="narL"
                     /locus_tag="ACHY03_001573"
     CDS             21861..22511
                     /gene="narL"
                     /locus_tag="ACHY03_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFS3904818.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(22512..23906)
                     /gene="ychO"
                     /locus_tag="ACHY03_001574"
                     /gene_synonym="iatA"
     CDS             complement(22512..23906)
                     /gene="ychO"
                     /locus_tag="ACHY03_001574"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFS3904819.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDTLSNDELRWEP"
     gene            24092..24445
                     /gene="ychN"
                     /locus_tag="ACHY03_001575"
     CDS             24092..24445
                     /gene="ychN"
                     /locus_tag="ACHY03_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFS3904820.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(24489..25184)
                     /gene="chaC"
                     /locus_tag="ACHY03_001576"
     CDS             complement(24489..25184)
                     /gene="chaC"
                     /locus_tag="ACHY03_001576"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFS3904821.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNELLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(25342..25572)
                     /gene="chaB"
                     /locus_tag="ACHY03_001577"
     CDS             complement(25342..25572)
                     /gene="chaB"
                     /locus_tag="ACHY03_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFS3904822.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            25842..26942
                     /gene="chaA"
                     /locus_tag="ACHY03_001578"
     CDS             25842..26942
                     /gene="chaA"
                     /locus_tag="ACHY03_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFS3904823.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         6885 a         7045 c         6517 g         6700 t
ORIGIN      
        1 ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg aaaataacat
       61 gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg agaagaatat
      121 ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa taatgacgaa
      181 gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca ggccggggaa
      241 aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca atagccccat
      301 aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc acggtgccat
      361 gcggaacaat ccccaacaac ataaagcgaa caatgcaata gccacaaaga aaccaaacag
      421 atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg cccctggccc
      481 cgccatcaac ggcaacgcca gtggcaccac accaatgctt tcacgtaccg cggtttctga
      541 tttttcttgc ttgttctgtt tatcctctcc aagcttgccg ctgatcatcg acatcgctat
      601 cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac caaaaagttg
      661 tagaatcgtg tcgccgagga aaagcgagat ccacaagata atggccacag acaggttggc
      721 tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc tgataaagac
      781 gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac
      841 gggaaaatca aaaagggtct gaatcacggt tagctccgaa gcaaaagccg gataatgtta
      901 gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca tttttcacct
      961 cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa aacatgctaa
     1021 tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg aaaagcgatg
     1081 ctgaaaggtg tcagctttgc aaatatttga tttggatcac gtaatcagta cccagaagtg
     1141 agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc taatgtttaa
     1201 actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt tcatcggtta
     1261 agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt actaaaaaag
     1321 tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt aacgcactcg
     1381 tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag caagtagaca
     1441 aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc gcgaaaatgg
     1501 ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac cactttgctt
     1561 ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg tctgaagacg
     1621 acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt atcgttccga
     1681 ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag acccgtaacg
     1741 ccattatctt ctccccgcac ccgcgtgcaa aagatgccac caacaaagcg gctgatatcg
     1801 ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg atcgatcaac
     1861 cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg atcctcgcga
     1921 ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct atcggtgtag
     1981 gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt gcagttgcat
     2041 ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa cagtctgttg
     2101 ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac ggcggctatc
     2161 tattgcaggg taaagagctg aaagctgttc aggacgttat cctgaaaaac ggtgcgctga
     2221 acgcggctat cgtaggtcag ccagcctata aaattgctga actggcaggc ttctctgtac
     2281 cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc gaaccgttcg
     2341 cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc gaagacgcgg
     2401 tagaaaaagc agagaaactg gttgctatgg gtggtatcgg tcatacctct tgcctgtaca
     2461 ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg aaaacggctc
     2521 gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat aacttcaaac
     2581 tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc tctgaaaacg
     2641 ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa aacatgttgt
     2701 ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc gcgctggatg
     2761 aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc ctgttcaaca
     2821 atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa actgaagtct
     2881 tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca gaactggcaa
     2941 actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg gacgccgcga
     3001 agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg gcgctgcgct
     3061 ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa gcgaaaatga
     3121 ttgctgtcac cactacttct ggtacaggtt ctgaagtcac tccgtttgcg gttgtaactg
     3181 acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg gatatggcga
     3241 ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc ggtggtctgg
     3301 acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag ttctctgatg
     3361 gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc taccacgaag
     3421 ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcgactatc gcgggtatcg
     3481 cgtttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg ggttcccagt
     3541 tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt cgctacaatg
     3601 cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg caggctcgcc
     3661 gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac cgtactgctg
     3721 ctaagatcga gaaactgctg gcatggctgg aaacgctgaa ggctgaactg ggtattccga
     3781 aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg gataaactgt
     3841 ctgaagatgc attcgatgac cagtgcactg gcgctaaccc gcgttacccg ctgatctccg
     3901 agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa ggtgaaactg
     3961 cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa tccgcttaat
     4021 cagtagcgct gtctggcaac ataaacggcc ccttctgggg ccgttttttt gtttacccaa
     4081 agtaactttt ccataaaccg acagcattag ccttcatcat atttgcgacg atgtataacg
     4141 cctaaacaca gggatagtgt actttacagg tcacaagtca acgtcggtgc ttaagagccc
     4201 tgtgaggcgt atagcggcgt taaaaactgc cgagaagggt gtatagcccg gaagaagtgc
     4261 gtaaaacgaa ctgacaggat aaaagtgccc tgctcaccct gtcagtaaag aaattcttat
     4321 taatcgtggc gatgcctttc ctgaatagcc gttaatgagc cgacttgtaa cgcctcttta
     4381 tagtgtttac ggcatacaga aacgtatcgt tcattaccac caataaccac ctgctcacct
     4441 tcgttataag gtctgcctgc ttgatcaaga cgcagcacca tgcttgcttt acggccacaa
     4501 aaacagatgg tttttaattc aaccagtttg tcggaccatg ccagtaagta ttggctgcca
     4561 ataaataatt cacctcgaaa atcggtacgt aaaccataac aaagtacggg tatatcgagt
     4621 tgatcgacaa cctccgataa ttcatatact tgttgtctgg ttaaaaactg gcattcatca
     4681 accagtacgc aatgaattgc ctgctgttca tgttccgcac gaatctcatc aaataatgat
     4741 gaattttggt taaataattt tgcaggcgat gacaaaccta tacgcgaact gactttcccg
     4801 gcaccaaagc gatcatcaat ttctgccgta tatacgacag tgcgcatgcc gcgttcctgg
     4861 taattgtatg aagattgcaa caatgctgta gacttacccg cattcattgc ggaatagtag
     4921 aaatatagct gtgccatcag ccacaggccc tcaatgatat gaaataaagt tggctggagt
     4981 ttatcataat tcgtcagttt ttcagctcaa cagtatgcac agaagtggta tatgcgttct
     5041 cccttacgaa gccttgcata atccttctga gctatcatta caactgcctc gcttgttata
     5101 agcgggtaaa tgactgctgg taactattca caatctttaa cctgttgcgc aagtaatagc
     5161 cctctgttga cctccaggag atagtgcaat actaagtccc tgctcttatt gcgactgttc
     5221 tacttttcat cattcgctta atagggaatt ctcgtaaaca cgactaatac agaagactga
     5281 aaggtcgtca gcctacgata atctccccat aaaatgtgac atgaatcagg aagttttaac
     5341 ctcacgtgct gcgaaatcat cggtacaaat agggctatat gccgcgtctt ttctggctaa
     5401 ttttatgaaa agatatttat tggcggcaca aaataaagaa caattttgaa ttccttacat
     5461 tcctggctat tgcacaactg aatttaaggc tctattatta cctcaacaaa ccaccccaat
     5521 ataagtttga gattactaca atgagcgaag cacttaaaat tctgaacaac atccgtactc
     5581 ttcgtgcgca ggcaagagaa tgtacacttg aaacgctgga agaaatgctg gaaaaattag
     5641 aagttgttgt taacgaacgt cgcgaagaag aaagcgcggc tgctgctgaa gttgaagagc
     5701 gcactcgtaa actgcagcaa tatcgcgaaa tgctgatcgc tgacggtatt gacccgaacg
     5761 agctgctgaa tagccttgcc gccgttaaat ctggcaccaa agctaaacgt gctcagcgtc
     5821 cggcaaaata tagctacgtt gacgaaaacg gcgaaactaa aacctggact ggccagggcc
     5881 gtactccagc tgtaatcaaa aaagcaatgg atgagcaagg taaatccctc gacgatttcc
     5941 tgatcaagca ataatctttt gtagattgca cttgcttaaa atcccgccat cggcgggatt
     6001 ttttattgtc cggtttaaga caatttaata agaataagct ataaaaaaac ggcgtcgatt
     6061 gctcaacgcc gtttcgtgga taacaccgat acggatgtta cttcttaatg cccatctctt
     6121 cttcaagcca ggctttaaat tccgtgccaa gggtgttatg acgaataccg tattcaacga
     6181 aggcctgcat gtaacctaat ttattaccgc agtcatggct cttccctttc atatgatagg
     6241 cttcaaccgt ttctttttcg attagcatat caattgcgtc ggtgagctga atttcatcac
     6301 cagctcccgg aggggttttt gccagcaacg gccaaatatc cgcactaagt acgtaacgac
     6361 ccacaatagc gagattagac ggcgcaacat ccgcttttgg tttttcaacc acgccaacca
     6421 tcggtacgct ttcacccggc gctaattcaa cgcctttgca atccacaacg ccatatgcgg
     6481 tcacatcagc aaccggttca accatgatct ggctatgacc cgtttcatcg aagcggcgga
     6541 tcatctctgc caggttatcc tgtgacaaat cggattcata ttcatccaga ataacgtcag
     6601 gcaaaataac agccacaggt tcatcaccca ctaccgggtg agcacacaat acagcgtgtc
     6661 ccaggccttt cgccagccct tgacgaactt gcataatagt cacgtgcggc ggacaaatag
     6721 actgcacttc atcaagcagt tgacgtttta cacgtttttc cagcattgct tccagttcga
     6781 aactggtatc aaagtggttt tcaatagagt ttttagatga gtgtgtaacc agcacaattt
     6841 cagtaatgcc agccgcaata cattcattca cgacgtattg aattaatggc ttatcgacaa
     6901 gtggcagcat ctctttcggg atggctttcg tcgccggcaa catcctggtt cctaatcccg
     6961 caacggggat aacggctttt ttgactttcg tattaatggc agccatttaa attctcctgg
     7021 actgttcgtg ttttgaacgt gttcatattt ctgtatcgca tcccagtata tcagcacccc
     7081 ggaggggcta agttctgaaa aggatgcgag ttagcataat taagacaatt acttatacga
     7141 atggcgacga tagtaccacc agccgacatt agctggtaat gcaaatttag cccgcgttat
     7201 cgtttgctca ttctgcagac aacatcaagc gcagccgacc accggttccc catatttggc
     7261 attgccaggc atcgcatcgc tggctcaatt gattcaaata agcgttacct aaagtgccta
     7321 acggaacacc attactgatt tgcacctggt gttcgccggt atttaacgtc gcattcagac
     7381 ccgcagaaac cagaatgaga tttttcagtt cgcgatgata atagccaact aatagcggaa
     7441 actgccccgg cagattggcc tgacggagta aatggtttac ctgcttcaat aacgcgccca
     7501 actctggcaa ccgttgattt tggtgtgcaa gctgttcctg taataatccg ttaaacaatg
     7561 cgcgtaataa caaggcagca agtacgccat tatgtccagc tcgggtgaca tcaaggcaat
     7621 aaaatgccag atcgttttcc gaaagtgcgg caatatcaag caccaggccg ggtttgtccg
     7681 cggcaaccaa ttgacgataa ttaacccggc attgggaaat cacctgctga actggcggtt
     7741 gtagttcctg taataatttc gccgctgcag cagggttatc aaccattgca tcccagtcgc
     7801 gaaaaagcct ttcctcttcc tcaacgcggg aattaaacat gctgggatag agacaggcaa
     7861 aaaccatctc gcgcaagcga ttcagatctt taaccggttt cagcaaaaca tcttcaacgc
     7921 ccagacgtaa cgctttggca atatctgcca tattttcagt ggcagatatc accagaactg
     7981 gtgtctggtc acctctgttg cgtatatgtt ccagcagttt aagcccgttc attcgtggca
     8041 tcgcgatatc acatatcatc aggtctggag tgaaacctcc cagcaactca agggcatcca
     8101 ccccatcagc cgccagtacc gttgtcgctc ccaatgagga aaaccacgaa tccagaagcg
     8161 agcgaaatac ctgctcatct tcaacaatga gaatctgttt tccgaccaat ggctgcgtca
     8221 tgttctctcc cctgactggc tttactcaat agtggcatgc tattgccacc tgcgcctgtc
     8281 agaattaact taagtgtaga taaaaattct ggtcaaatgt tggtgcgtac caacggcaaa
     8341 agttcgtcca ttttcttttc cactgccagc tgcccggccg caatggcagc gtgcgcgcga
     8401 tggaaatcaa gcgtagatat ttgcgggcaa acaggttgaa tcagaatatc gggcggatca
     8461 cctgccatgc ggttcctttt aaggcggttc tccagcacct ggatagaagt ggtcataatc
     8521 tctgttgccg ttggtgctgt caccgcccga cgtgtcgtta tgctgcctaa cctttctttc
     8581 agacgcgcat gccacggcag agaatcacca ttctcgctat tttcttcact gacattaaag
     8641 gagagcaaat cttgttgcat caaatgagca tcgtgctgca ggtcgaccgc tatcacaata
     8701 tcagcaccca atgcacgcgt gagcgaaata ggaattgggt taacgactgc cccatcaacc
     8761 agccagtagc cgttatgcgc aacgggtgcc atgagtcctg gaatactgca tgatgcgcga
     8821 atagcaagat ggagatcgcc ttcagtaaac cataattcac gcccagtact taaattggtg
     8881 gcaacagccg cgaagcgacg ggaacaattt tcgatctctg tttccggcat tatttcgcga
     8941 tattgattga agacacgctc gccgcgcagt aacccaccgc gctgccagga gagatccatc
     9001 aggcgtaaaa catcccaata actgaaagag gttacccaat cttccagcgc agataatcga
     9061 tcgcatgcat aggccgcgcc caccagcgaa ccaattgaac atcctgcaac gatatcaatt
     9121 tcaataccca ctttttttag cgcattaata acgccaatat gcgaccaacc tctcgccgcg
     9181 ccagatccca gcgccagccc tatttttatc tttctcatta ttcccgcaag attcccctgg
     9241 aatgcaatcg tagccacatc gctactgctc ggttaacata gtgctactca ggcggttaat
     9301 acgccgttat tgtttccagg gagattattt gtgtctcagc tttgtccctg tggtagtgct
     9361 gtcgagtata gcctatgttg ccacccttat gtgtctggtg aaaaggttgc acctgatcca
     9421 gaacatctca tgcgttcgcg ttactgcgct tttgtgatgc aagacgcaga ttatttaata
     9481 aagacctggc atccatcttg tggagcagcg gcgttacgtg ccgaattgat tgccggattt
     9541 gcgcatactg aatggttggg attgaccgta tttgaacatt gttggcagga cgccgacaat
     9601 atcggatttg tcagttttgt tgctcgattt accgaaggtg gcaaaacagg tgcaataatt
     9661 gaacgttcgc gatttttgaa agaaaacggt caatggtact atattgacgg tacacgtccg
     9721 cagtttggcc gcaacgatcc ctgcccttgt ggttcaggta aaaaatttaa aaagtgctgc
     9781 ggccaataat ggttgacggt acggtttagc aaacactctc aacaaggttt ttccagcaat
     9841 gcattcactc caacgtaaag ttctgcgtac tatttgtccg gaccaaaaag gtctgatcgc
     9901 acgtattacc aatatttgct acaagcacga gttaaatatc gtacagaaca atgaatttgt
     9961 tgatcaccgt accgggcgct tttttatgcg cacggaactg gaagggattt ttaatgattc
    10021 caccctgctg gcggatctcg atagcgcatt gccagaaggc tccgtgcgtg agctgaatcc
    10081 tgccggtcgt cgccggatag tgattctggt cactaaagaa gcgcattgcc ttggcgattt
    10141 gttgatgaaa gccaattatg gcggcctgga tgtcgaaatc gcggcagtga ttggtaacca
    10201 cgatacttta cgttctctgg ttgagcgttt tgatataccg tttgagctgg taagccatga
    10261 agggttaagc cgcaacgagc acgatcaaaa gatggcggat gccattgatg cttatcaacc
    10321 tgactacgtg gtgctggcga agtatatgcg ggtattaaca ccggaatttg tgtcacgctt
    10381 cccgaataag atcatcaata ttcaccattc cttcctgcca gcgtttatcg gcgcacgtcc
    10441 ttatcaccag gcctatgaac gtggcgtgaa gattattggc gcaacagctc actatgttaa
    10501 tgacaatctg gacgaaggcc caatcatcat gcaggacgtt attcatgtcg atcataccta
    10561 cacagctgaa gatatgatgc gcgcaggtcg tgacgtcgag aaaaacgtct taagtcgcgc
    10621 gctctacaaa gtactggcgc agcgcgtctt tgtttacggt aatcggacga ttattcttta
    10681 atcgccagca aaaataactg gttaccttta atccgttacg gatgaaaatt acgcaaccag
    10741 ttcatttttc tcaacgtaac actttacagc ggcgcgtcat ttgatatgat gcgccccgct
    10801 tcccgataag ggagcaggcc agtaaaaagc attaccccgt ggtggggttc ccgagcggcc
    10861 aaagggagca gactgtaaat ctgccgtcat cgacttcgaa ggttcgaatc cttcccccac
    10921 caccatcact ttcaaaagtc cctgaactct caaacgaatc cgtaattaaa tattctgccc
    10981 aagcggggaa ggatgagaag cttcgaccaa ggttcgactt gagcgccagc gagagagcgt
    11041 tgccgcaggc aacgacccga agggcgaagc gcgcagcgct gagtaatcct tcccccacca
    11101 ccatcgcttc ctgaaataat caaaacctgt tactccgagc taattttgcc caatggcacc
    11161 acgtttaccg gacctacaat atcctcccct gtaggtcgga taagcgaagc gcatccgaca
    11221 actcaccccg caccgtgccc ataaaaaaac cccgccgaag cagggtctat gtgaactaaa
    11281 attcgcttag tgacgagcgc gcaccagctg gtacttacgt gtcagatact ccaccggtac
    11341 gctccagatg tgtaccagac gcgagaacgg gaacagcagg aataacgtca tccccagcac
    11401 caggtgtaga cggaagataa acgccacacc atcgaggtgt tgagaagcgc caccgtggaa
    11461 ggtcaccacc gactgcgccc agccaaccag tttcatcatc tcgctaccgt ccatatgctg
    11521 agcggagaat ggaatggtca gcaggcccag cgcgcactgg ataacgagca gcgacaggat
    11581 caggatatcc gctccggtag tggttgcacg cacgcgtggg ctgaacagac gacgcttcag
    11641 caacagcacg ccgccaatca gacacagcac accgctggca ccaccagcaa acatcgccat
    11701 tttctgtttc acttcaatcg gcagccaggc ttcatacatc cagtgcggcg tcagcatacc
    11761 gaagaagtga ccaacaaaaa tccccagaat accgatatgg aacaggttcg acgccaggtt
    11821 catccctttg cgatccagca tctggctgga cgccgcgcgc caggtgtact gcccgtagtc
    11881 ataacgcagc cagctaccaa tcaggaagac cgccccggct atgtacgggt agatatcaaa
    11941 gaagaacata ttcaggaatt gcattagtgc tgtcctccgg tggtgatatt cagatattgc
    12001 ggcgcgacgg caccggcaaa gcgacgctga tgagcagtga ttgctgaatc accgcagcct
    12061 ttgtcagcaa agaatttaac ctgctcttct tcccagacag catccagcgc ctgcggcgta
    12121 tcgtcgcgcg cttcgtcggc aattttttcc gccactttgt cgctgtcgat agcggtattc
    12181 gccagtttca tcagcagatc aaacagcacg gcataacggc tttcacgctg ttgcagacgc
    12241 gcgctcagca atgccagaat cggcgcgata tctttcaaac cttccacggc ttcgctttgc
    12301 ggtagctgcg ccaggtactc cagatacagc ggcagatggt ccggcaattc gcggctgttt
    12361 aactgcaagc cgtgctgctc gtactgcgcc agcaggtcca ccatcgcctg gccgcggtcg
    12421 cgggattcgc cgtgcacatg ttcaaacagc aacagtgacg tggcgcggcc acggtcgaac
    12481 agttcgctgt actgcgcctg ggcatcgagc ggatccatcg tcgttaaatc gcgcaggaaa
    12541 atgcccagcg catgggcatc ctcttttggc agatttttcg acgcggcaat cgcctcaaac
    12601 atctcttgtt gatgctgcca taaggcagca tccggatatt caaggagacg cgatacaatc
    12661 acgagttcga tcatggatgc ggctccgttt tgctggtcac atcgatggca tcgatacgac
    12721 ggctgttgaa cagattgaat ttggtatctg aaccgtggca gccatcacca aaggtaaagc
    12781 cgcagccatt tttctccggg aaggcttccc gtgccagttc acgatgacta ctcggcacca
    12841 caaagcgatc ttcgtagtta gcaatcgcca gataacggta catctcctgt gcctgggctt
    12901 cggtcagacc gacctcttcc agcgcacggg tatccacttt cccgtcaacg gtttcagcac
    12961 gtttgtaatg acgcatcgcc agcatacgtt tcagcgcgcg cagtaccggt ttggtatcac
    13021 cggcggtcag cagattcgcc agatactgta ccggaatacg caggctttcg acgtctggca
    13081 gaatgccgtt gctacccagc tcacccgcgt ctgctgcaga ctgaatcgga gacagaggcg
    13141 gcacgtacca gaccatcggc agtgtgcgat attctggatg cagcggcagc gccagtttcc
    13201 attccattgc cattttataa accggcgact gctgtgcagc ttcaataacg ctcagcggaa
    13261 taccgtcttt aatcgcctgc tcgatgactt tcggatcgtt cggatcgagg aacacctcca
    13321 gctgacgctg gtagagatct ttctcgttct cggtgctggc tgcacgttca atcgcgtcgg
    13381 catcatacag cagcacgcca agataacgga tacgaccgac acaggtttct gagcacacgg
    13441 tcggctgacc cgcttcaata cgcggatagc agaagatgca cttctcagac ttaccgctct
    13501 tccagttgaa gtagattttt ttgtacgggc atccggtgat gcacatacgc cagccacggc
    13561 atttatcctg gtcgatcagg acgatgccat cttcttcacg cttgtaaatc gcaccgctcg
    13621 ggcaggtcgc cacacatgcc gggttcaggc agtgttcgca caggcgcggc aaatacatca
    13681 tgaaggtgtt ttcgaactgg ctatacatcg ccttctggat gttgtcgaag ttcttgtctt
    13741 tcgccagttt gtcaaactca ccgcccaggt catcttccca gttcggccct ttttcgattt
    13801 tcgccatccg ttccccggta atcagcgaac gcggacgggc aatcggctgc gatttgctgc
    13861 cttccggcgc ggtatgcagg ttctgatagt caaaatcgaa cggctcgtaa taatcgtcga
    13921 tccccggcaa atgcgggtta gcaaagattt tacccagcag catggctcgg ttacccatgc
    13981 gcggttgcag tttgccattg attttacgga tccagccgcc tttgtatttt tcctggtttt
    14041 cccagtcagt cgggaagccc tggcccggct tggtttccac gttgttgaac cacgcgtatt
    14101 ccacgccttc acggctggtc cagacgtttt tacaggtaac tgaacaggtg tggcagccga
    14161 tgcacttatc gagattcagc accatgccga cttgtgaacg aattttcatt ttacgctctc
    14221 ctgtacctgg tcattgcctt cgccatctaa ccagtcaatg ttcttcatct tacgcactac
    14281 aacaaactca tcgcggttag aacctacggt gccgtagtag ttaaagccgt atgccagatg
    14341 ggcatagccg ccgatcatat gcgtcggttt cggcgtgata cgggtgaccg agttatggat
    14401 accaccacgc tgttgggtaa tttccgaacc aggcaggtta acgatacgtt cctgcgcgtg
    14461 gtacatcatg gtcatccctg ccggaacacg ctggctgaca accgcacggg cagtcagagc
    14521 accgttgctg ttgaatactt caatccagtc gttatcggcg atacccagat ctttggcatc
    14581 ggcttcactc aaccagacca ccggaccacc gcgacctaaa gtcagcatca gcaggttgtc
    14641 gctgtaggtg gagtggatac cccacttctg gtgcggcgtc aggaagttga gcgctttttc
    14701 cgggttgccg ttggatttct ggcctataac ttctttcacc gaacgggtgt cgatcggcgg
    14761 acgataaacc agcaggcttt caccgaaatc acgcatccac tggtgatcct gatacagttg
    14821 ctgacgacca gagagcgtac gccatgggat cagctcgtga acgttggtgt aaccggcgtt
    14881 gtaagaaacg tgttcatctt ccagaccaga ccaggtcggg ctggagataa ttttgcgcgg
    14941 ctgtgcctga atatcgcgga agcggatctt ctcgtcttct ttattcagcg ccagatgcgt
    15001 atggtcacga ccggtaaatt cgctcagggc agcccaggct ttcacggcta cctgaccgtt
    15061 ggtttccggt gccagtgtca ggatcatctc tgccgcatca attgcggtgt tcagcatcgg
    15121 ctggcctttc gccggacctt ccgctttggt gtagttgagc ttacgcagca gatccatctc
    15181 gctctgggtg ttccaggcaa tccctttacc gccgttaccg attttctcca tcagcgggcc
    15241 gatagaggta aagcgttcgt aagttgccgg ataatcgcgc tctacgacca taatgtgcgg
    15301 tgcagttttg cctgggatca ggtcgcattc gccttttttc cagtctttca catccagcgg
    15361 ctgcgccagt tcagcggcag agtcgtgctg gataggcagc gtgacgatgt cggtttcttt
    15421 acccagatgg ccgacgcaca cttcggagaa tttcttcgcg atggctttgt agatttccca
    15481 gtcgcttttc gcttcccagg ccggatcgac cgccgcagac agcgggtgaa taaacggatg
    15541 catatccgaa gtattcatgt cgtctttttc gtaccaggtc gccgtcggca aaatgatgtc
    15601 ggaatagagg caggtgctcg acagacggaa gtccagcgta accaccagat ccagcttgcc
    15661 ttccagacca ttgtcctgcc agtccacttc ttccggcttc acgccgccct gctgccccag
    15721 atctttaccc tggataccgt gttccgtccc cagcaggtac ttgagcataa actcatgacc
    15781 tttaccggaa gaaccgagca ggttagaacg ccagatgaac aggttgcgcg ggtggttttt
    15841 accgttttct ggttgttctg ccgcaaaacg gatggaaccc tctttcaggg atttcaccgt
    15901 atagtccacc ggattcatcc cggctttttc cgcttcgcca gcgatagtca gcgggttagt
    15961 gcctaactgc ggtgcagacg gcagccagcc catgcgttcc gcacggacgt taaagtcgat
    16021 caggtgtccg gtataacggg atttatccgc catcggtgac agcagttctt ccgcagtcac
    16081 ggtttcataa cgccactggc tggagtggtt atagaaataa gaagtgctgt tcatgtgacg
    16141 cgccggacgc tgccagtcaa gggcaaacgc cagcggctgc cagccggttt gcggacgcag
    16201 tttttcctga cctacatagt gcgcccagcc gcccccgctc tgaccgacac agccgcagaa
    16261 aatcagcatg ttgatcagac cacgatagtt catatcgagg tgataccagt ggttcagccc
    16321 cgcaccgacg ataatcatcg aacgaccgtg cgttttatca gcgttatcgg caaattcacg
    16381 ggcgatgcga ataatctggc tgcgagaaac gccggtaatc tgctcggccc aggccggggt
    16441 ataagctttc acatcgtcat agctggttgc acagttaacg tcgttcaggc cacgttccag
    16501 accgtagttt gccagcgtca gatcataaac ggtggtcacc agggcggtgc tgccgtcagc
    16561 cagtttcagg cgtttcaccg gcagtttgtg cagcagcacg ttttccagtt ccactttgtt
    16621 gaagtgttcc gtgccgtcgc caccaaagta cgggaagcct acctcagcga tctcatcctg
    16681 gctacccagc aggctcagtt gcagctcggt ttcttcgcca gttttgccgt cgcgctgctc
    16741 aagattccat ttgcccttct cgccccagcg gaagccaata gaaccgttcg gcgcaaccat
    16801 ttcgccattg gtattaaagg cgacagtttt ccattccgga ttgttttcct ggcccagcgc
    16861 atcaaccaga tcagcagcgc gcagcatacg acctgcagcg tagtaaccgt cgcgttcttc
    16921 cagcatcacc agcatcggca tgtcggtgta gcgacgcaca tagtcggtga aatactggct
    16981 tgggttgtcg aggtggaatt cacgcagcat tacgtggccc atcgccagcg ccattgccgc
    17041 atcggtgccc tgtttcggtg ccagccacag atcgcacagt ttggcgattt cagcgtagtc
    17101 tggtgtgacg gcaacagttt tggtgccttt gtaacgcact tcagtaaaga agtgagcatc
    17161 cggggtacgc gtctgcggca cgtttgaacc ccaggcgatg atgtagctgg agttgtacca
    17221 gtcagcagat tccggtacgt cagtttgctc gccccaggtt tgcggagacg caggaggcag
    17281 gtcgcagtac cagtcgtaga agcttaagca agtaccgcca atcagcgaga gatagcgtgc
    17341 acccgatgcg taagaaacca tcgacattgc cggaattggc gagaaaccag caacacggtc
    17401 cgggccgtag tttttgatgg tgtaaacgtt agatgcggcg atcagttcgt tcacctcctg
    17461 ccaggaagaa cgaacaaaac caccgcgtcc acgcgcctgc ttaaagcttt tcgctttatc
    17521 ggcgtcttca atgatagaag cccatgcctc aaccggatcg ctatgcagcg ccttcgcttc
    17581 acgccacatt ttcatcaggc gtttgcgcat catcgggtat ttcaggcggt tggcactgta
    17641 aagataccag gagtagctgg caccgcgcgg gcagccgcga ggttcatggt ttggcagatc
    17701 cggacgggta cgcggatagt cagtctgctg ggtttcccag gtgaccagac cgtttttgac
    17761 gtagattttc cagctgcagg agccggtgca gtttaccccg tgggtagagc ggacgatttt
    17821 gtcatgctgc caacgctggc gatatccatc ctcccagtca cggttggtat tgagaagctg
    17881 gccatgccca tcggcaaagg tttcaccctt ctgtttgaag tagcgaaacc ggtccaggaa
    17941 tttactcatc ggttttctcc tgtgggagcc tgtcggccct ctgataaatc gacattgcta
    18001 atgtttaagg gcaaggtaac gctctgaaac agcgtgggaa ttgataacga tcaagagtga
    18061 tggggatgaa aaataaagta aattcctaag ataccccttt aggataatta acagttacag
    18121 cataacatgc tgtaaattaa gacgttttat acctgtagtt atttaaacta ttaagatttt
    18181 tatgcaaatg ggtattaagg agtattcccc atgggtaaca tattaatggc tcttaatgaa
    18241 atccggcgaa ataaaccata taacattagt atgttgtaac taaatgacgc ataaaaaaag
    18301 cgcgacatca tgccgcgcaa aggataatca aatattaaga tttatttttt agaatgccga
    18361 ccatataccg cccaggtaat cactacgcag gcgatataga aaatcaaaaa taccttcatt
    18421 gcgccgactg gcgaacccgt taatgccagc gagctaccaa acgctttcgg gataaagaag
    18481 ccaccaatcg cgccaatcgc agagatgaaa cccaacgccg ccgccgtgtc ggttgccgct
    18541 tcacgcatcg cacgttcgtc agaaccccct tctgctttca cgcgatccat tgtcagttta
    18601 cggaagatca cggaaatcat ctggaaagtg gaaccactac ccagcccagc tgtcaggaac
    18661 agcgccagga agaccgcgaa gaacgccatg aagcttccgc cctgcccgtc agtcggtaag
    18721 gtcaggaaca gcaggccgct gaaaatcgcc atcagaataa agttcaccag cgtgacacga
    18781 gttccgccca gacggtcaga taatgcacca cctgcagaac gcgccagcgc accaataaac
    18841 ggcccgaaga aagcgtattg cagaatctga acatccggga actgcgtttt tgacagcatc
    18901 gcaaagcccg cggagaagcc gataaaggag ccgaaggttg ccagatacag caggctcata
    18961 atccacagat gaccccgttt gagtaccggc aactgctcct tgatggaggc tttcgaggta
    19021 gcaagatcgt tcatgccaaa ccacgccgca atggtgaaga tggcaaggaa cggcacccat
    19081 atccaggacg cattcgccag atacagctca gtcccatccg gctgtttgac gccctggcta
    19141 ccaaatactg cgaaaatcga cagtgatacc accagcggag caaccaactg catgacgctg
    19201 acgcccatgt tgcccagacc accattcaga cccagcgcgc caccctgctt ctgtttcgga
    19261 aagaagaagc tgatgtttgc catactggaa gcgaagttcg cgccagcaaa gccgcacagc
    19321 agagagatga tgatgaagac gctataaggc gtggaggtat cctgcacggc aaaacccagc
    19381 cagacacaag gaataatcag aataccggtg ctgaacgccg tccagcgacg accaccgaag
    19441 ataggaacca taaaggagta tggaacacgt aataacgcgc cagaaaccga aggcagcgca
    19501 gtcaacataa atagctgatc ggtcgtaaaa ttaaaaccga ctttcggtaa gttcaccgca
    19561 acagcgctga acaacatcca tacgcaaaac gccagcagca gacagggaac ggaaatccac
    19621 aggttgcggc tggcaatacg ttgaccgcgt tgttgccaga acgcaggatc ttccggtcgc
    19681 caatctgtaa tgacagctcc agtagccctt tcgggggcgg atgagtgact catagacacc
    19741 tctgatactc gtttcgatgt ctgccacctt agtgtctgta gctaaaggca atttgatgta
    19801 aatcaaacga taagataact ttatctatca ttgatattta tcattaccca tagtgagtac
    19861 agtgatttca taaaaattat gagatttttc acggtgctgt aaaatcccta cccttaccga
    19921 tgtaaagcga ctaaccacac ggcaaataag gagtaactct ttccgggtat gggtatactt
    19981 cagccaatag ccgagaatac tgccattcca gaatgcatcg tcacattcat taaggttatt
    20041 gctcatttaa agcctgaagg aagaggttta catgcttaaa cgttgtctct ctccgctcac
    20101 cctggttaat caggttgcgc ttattgtgtt gctttctact gctattggac tggcagggat
    20161 ggcggtttct ggctggctgg tgcaaggcgt tcagggcagc gcccatgcga tcaacaaagc
    20221 gggatcgctg cgcatgcaaa gttaccgtct gttggcggca gtgccattaa gcgagaaaga
    20281 caaaccctta attaaagaga tggaacaaac ggcatttagc gccgagttga ctcgagcagc
    20341 agaacgagac ggacaactgg cgcaattaca gggtttacaa gattactggc gtaatgaact
    20401 gatccctgcg ctgatgcgtg cacaaaaccg agaaacggtg tcagcgggtg tcagccagtt
    20461 tgttgccggg cttgatcagc tggtatctgg ttttgaccgc accacggaaa tgcgcattga
    20521 gacagtggta ctggtccatc gggtaatggc ggtatttatg gcacttttac tggtgttcac
    20581 tattatctgg ttgcgggcgc gactgctaca accgtggcgg caactgctgg caatggcgag
    20641 tgccgtcagc catcgcgatt ttacccaacg cgcaaacatc agcgggcgca acgaaatggc
    20701 gatgcttgga actgcgttga acaatatgtc tgcagaactg gccgaaagtt atgccgtact
    20761 tgagcagcgg gttcaggaga aaaccgccgg gctggagcat aaaaatcaga tcctctcttt
    20821 tttatggcag gctaaccgcc gtttgcattc ccgcgccccg ctgtgtgaac gcctgtcacc
    20881 tgtactcaac ggcttacaga atttaaccct gctacgtgat atcgaactgc gggtgtatga
    20941 cactgatgat gaagagaatc atcaggagtt tacctgccag ccagatatga cttgtgatga
    21001 taaaggctgc cagctctgcc cgcgcggcgt attacccgtt ggtgatcgcg gcacgaccct
    21061 gaagtggcgg ctggctgact ctcatacgca gtacggtatt ttgctggcga ccctgccgca
    21121 ggggcgtcat cttagccatg atcaacaaca actggtggat accctggttg aacaactcac
    21181 cgccacgctg gcgctggatc gccatcagga acgtcagcaa cagttgatcg tgatggaaga
    21241 gcgtgccacc attgcgcgcg aactgcatga ttctattgcc caatctctct cttgcatgaa
    21301 gatgcaggtg agttgtttac agatgcaggg cgatgcgctg ccagaaagca gccgcgaact
    21361 gttaagtcag atccgtaacg aactgaatgc atcctgggcg cagttgcgtg aattgctcac
    21421 cacattccgc ttgcagctca ccgagcctgg attacgtccg gcgctggagg cgagttgcga
    21481 agagtacagc gccaaatttg gcttcccggt gaagctggat tatcaattgc cgcctcgtct
    21541 ggtgccttcg catcaggcaa tccacttgtt gcaaattgcc cgtgaggcat taagtaacgc
    21601 cctcaaacat tcgcaagcga gtgaggtcgt ggtgacggtg gcgcaaaacg ataatcaggt
    21661 caaactgacc gtccaggata acggctgcgg cgtgcctgaa aatgccatcc gcagcaatca
    21721 ctacggcatg ataataatgc gcgaccgtgc gcaaagttta cgaggcgatt gccgcgtccg
    21781 ccgtcgtgaa tcaggtggca ccgaagtggt ggtcaccttt attcccgaaa aaactttcac
    21841 agacgtccaa ggagataccc atgagtaatc aggaaccggc tactatcctg ctgattgacg
    21901 atcacccgat gctgcgaact ggcgtaaaac agcttatcag tatggcacca gatatcaccg
    21961 tggttggcga agcgagtaat ggcgaacagg gtattgaact ggcggaatct cttgatcccg
    22021 atctgatcct gttagatctc aatatgcccg gcatgaacgg tctggaaacg ctggataaac
    22081 tgcgcgaaaa gtccctctca gggcgcattg tggtattcag tgtctctaac catgaagaag
    22141 atgtggtcac cgcactgaaa cgcggcgcgg atggctatct gttaaaagat atggaaccgg
    22201 aagatctgct gaaagcattg catcaggcag ctgctggcga aatggtatta agcgaagcat
    22261 taacgcctgt tctggccgcc agcttgcgcg ctaaccgtgc cacgactgag cgcgatgtta
    22321 accagttaac cccacgcgag cgcgatattc tcaagctgat tgcccagggt ttgccgaaca
    22381 agatgattgc ccgccgcctg gacatcaccg aaagcacagt aaaagtgcac gtcaagcaca
    22441 tgctgaagaa aatgaagctc aagtctcgcg tggaagcagc ggtatgggtg catcaggagc
    22501 gcattttctg attacggttc ccagcgcagt tcgtcgtttg acaatgtgtc gaacggttcg
    22561 acaagcgtta gcgtgatctc attggaggag acacgctgcc cctggttatc ttccaccacc
    22621 accgacaatc gccagtgatt gctcgcccct tccccgttct gccagtcagg catgatcagc
    22681 gtccagccct ccgcgctgtt ggcttgtgcg cccggcgtca aacttaatat ctgcgtatcg
    22741 ccctgccaaa tcagttgccg aataccgtaa cgactgcgga tttgtaattt cagcggcact
    22801 gtttcgccag gttttagatc ccacggcggt gtcgccagaa acaccgttaa cgtttttcgc
    22861 tgtcggtact caagagtcgg tagattattt cgctgcggat tatcatagcg actaccacgt
    22921 aacgactgac tttcggcaac ctcgcccgca gaaagttgtt ttttgagcgg tacaccaaag
    22981 cggtaattaa gattcagccc gagattattt tgattctcgc cactttcacc ctgtttatgc
    23041 tgggccgtca cagtgactaa tggcacaggg gtgtaattta atcccagact caacgcgacg
    23101 ggattgtgat aacccgtacc agagttaaac aaatcaacac gatcaccaaa atactgttct
    23161 acgctgacac tggtattgag gtgttgatag aacggcatgc gcatccgggc tgtcaggtcg
    23221 tacccgcgcg ccatccgttg ttcctgcgtg gctgtctgtt catgccatgc agcaaacggc
    23281 tgataaaagt ttgccgatag tcgcaaatat tcgccccacg cttcggcacc aaagcccgct
    23341 cgctgaagat tttcgtccag caagttgtca taaaaagtgt tataacccac cagccagttg
    23401 ccgcgcgccc agcgttgccc aacgcccaca ttgctcacca acccatcatc ctgctgagta
    23461 agaccaagct ggctccaggt gagataacga tcattatctt gtaacggcac aaaccagctt
    23521 ccacgactgc cggtgaaatg tccttcgtta tccactttga cgtcaacact ggcatttccc
    23581 cacggtgata gccaggactc tacgtgctga ttaacctgtt gactaagcgc gtcgcggact
    23641 tttcccaatg cgaaagcttt tgcctgctcg ccagtatcca gcccgttatc attcatactg
    23701 gtttcgccga aatctttcac aatttcagca aagtgttttt ccccatcatg attttcaggt
    23761 gccatgccta aatccggcag accatcattg ttattatcaa agggatttgc cgctttttgc
    23821 tcgaaggtag attgtgcgtt agctgtaccg cctgccgcca gcaagagtaa ataaaacgga
    23881 ataatgcgag gaacgaaacg gctcaaaacg tcggtaactt ccggtaaaaa atgaaaaacc
    23941 atagccttaa agaataacga ttactggcaa cttttgtcgt attttctcgc gctttcagga
    24001 aaatccttaa aaatgtgacc gattggtcag acgctgcaga agcagtaaga tagaagatga
    24061 gtgtctaatt ccctgaatac aggagtaaat aatgcaaaaa atcgtgatcg ttgccaatgg
    24121 cgcaccttac gggagcgaat ccttgtttaa cagcttgcgg ctggccattg cgttacgaga
    24181 gcaggagagc aatctggatc tgcgtctgtt cctgatgtct gatgcggtca cagccgggtt
    24241 gcgcgggcaa aaaccagggg aaggctacaa catacagcaa atgctggaga tccttaccgc
    24301 tcagaatgta ccggtgaaat tgtgcaaaac ctgtaccgac gggcgcggga ttagtacact
    24361 tcctctgatt gatggggtgg aaatcggtac tcttgtggaa ctggcgcaat ggacgctgtc
    24421 agccgataaa gtgctcacat tttaatgttt gagggggcgt ctgacgcccc acgatgaggg
    24481 aagtttactc aggcgaatcc cggccgcaac acaccatccg gataattctc cgccagcagt
    24541 ttttttaccg ataccagcaa ttcattcagc ccatcgtcct gcattcccag tttgataagc
    24601 tcctgttcca gtgaaaacag gtattgtgcg ttggttccca gcggaccgct cgccgccgca
    24661 atcaacggcg cgatgacctg agcgcgagtg tcagattcat attctggatg tcgcgggtcc
    24721 ataataaaca caatggcgtt tactgtgcgt ccatcatcaa gatcaagctg acaccaggtt
    24781 ggcagataac agccggtaat catctcgcgc ttccacaaca gggttagttc ctgctccagc
    24841 gtctcttctg gcagtcgata ggcgacgcct gtggtgcgtc cgccctcttt cagtgcaagc
    24901 atccgtcccg gctggtgcgc agtcccgcgc ccagcggtca ggcgcaggca aaatgcgcga
    24961 tgccatccaa ccagtgtacc ggtgcacgac tcggtaaact ccagtgccgg attccacatc
    25021 aacgatccat aaccgaagat ccacaccggt ccctcatcag gtcgacaagc cagcgtcgcc
    25081 gccagcgaag ccgcccgttg ttctgctgac cataagagtg attcttcaat ggcaccaaat
    25141 gccgttttac aatcggcatt catcaagaaa tcacgcgtta tcaccttaca cctccgccac
    25201 tgcatacttc cctgcgacaa ctttttggtg tcttccggac atttttactg ctcattttga
    25261 aaccactaac gggacaatat gcaatccacg gaccacttgc aaggcaacac cgcacgaata
    25321 gctttaacta agctaccggt tttacgattt tttatgccat ttatcatcat cccctttggc
    25381 atattcatgc ttcacagcag cccaggccac tttatgcgcg gtttcttcgc gactggcgtc
    25441 atcacgccga tcttctttat ctttatattg atcccatgcg ctgttgaacg cttctttata
    25501 gatatcctgg gcatgagacg gtagaacgtg ctttacgctt tccggcaggt cgcttttcgt
    25561 tttatacggc atcgttgact ccttttacaa agttgaacat taagtgtggt caacaatatc
    25621 tactcttgcc aaaacataag aattttcatg tcagactgtt cttaagaaac gctaaaacaa
    25681 actttgtcta aacgtattac ttatttttat gccccaatga gtaaaaaatc gacaatgggg
    25741 taaaaattcg taaaatgtta ccgatatcca gacactttct gttatttttc ctacctattt
    25801 tttacatttt cgataaattc atctgcaaag ggataaccat aatgtcaaat gctcaagagg
    25861 cggtaaaaac ccgccacaag gagacttcgc ttattttccc ggttctggcg ctggtagtgc
    25921 tgttcctgtg gggaagcagc cagacactac cagtggtcat tgccatcaat cttcttgcgc
    25981 ttattggtat tttaagtagc gcctttagtg ttgtccgtca tgcggacgta ttagcccatc
    26041 gcctgggaga accttacggt tcgcttattc ttagcctttc agtggttatt cttgaagtta
    26101 gtttgatttc agctttaatg gcaaccggcg acgccgcgcc aacgctaatg cgtgatacgc
    26161 tctattcgat cattatgatt gttaccggtg ggctggtcgg cttttcatta ttgttgggcg
    26221 gtcgtaagtt tgccacccaa tatatgaatc tgtttggtat taagcagtat ttaattgcgc
    26281 tgttccccct agcgataatc gtactggtat ttccaatggc tctgcctgcg gcgaattttt
    26341 caaccggtca ggcgttactg gtagcattaa tttctgcggc aatgtatggc gtatttttgc
    26401 tgatccagac caaaacgcat caaagtttgt ttgtctacga gcacgaagat gacagtgatg
    26461 acgacgaccc gcaccacggt aaaccgtctg cccatagcag cctgtggcat gctatctggt
    26521 tgattatcca cctgattgcc gttattgcag tcaccaaaat gaacgccagc ccgctggaga
    26581 ctttgctcga cagcatgaat gcccctgtcg cctttactgg cttcctggtg gcactgttga
    26641 ttctgtcgcc ggaaggttta ggtgcattaa aagcagtgtt gaacaaccag gtccagcgcg
    26701 cgatgaatct gttctttggt tcagtgttag caacaatttc gcttaccgta cctgtcgtca
    26761 cgctaattgc atttatgacg ggtaacgaat tgcagtttgc acttggtgcg ccagaaatgg
    26821 tggtgatggt ggcctcttta gtgctgtgcc atatctcctt ctccaccgga cgtactaacg
    26881 tgctcaatgg cgcagcgcat ctggcactgt ttgccgccta tttgatgacg atatttgcct
    26941 gacagttata tctcctccga tgacggaaat cgggggagat aatataaatt cctgcttatt
    27001 gtatttcttc atactacctg gacaggtcgt taatcagaat tgcgagcctg taccacaacc
    27061 tgcacgaaac cactgcaaca gctgttgaaa agccggaatg tactctttat tgcaaacatt
    27121 tcagggatga agtgaagatg aattgag
//
LOCUS       DAZUCE010000044        26852 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZUCE010000044 DAZUCE010000000
VERSION     DAZUCE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.044"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            59..334
                     /gene="frmR"
                     /locus_tag="ACHY03_001579"
     CDS             59..334
                     /gene="frmR"
                     /locus_tag="ACHY03_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFS3904824.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            369..1478
                     /gene="frmA"
                     /locus_tag="ACHY03_001580"
     CDS             369..1478
                     /gene="frmA"
                     /locus_tag="ACHY03_001580"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFS3904825.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            1571..2404
                     /gene="fghA"
                     /locus_tag="ACHY03_001581"
     CDS             1571..2404
                     /gene="fghA"
                     /locus_tag="ACHY03_001581"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFS3904826.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMHHFPATTKKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTQNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(2529..3068)
                     /gene="yaiL"
                     /locus_tag="ACHY03_001582"
     CDS             complement(2529..3068)
                     /gene="yaiL"
                     /locus_tag="ACHY03_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFS3904827.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(3170..4381)
                     /gene="mhpT"
                     /locus_tag="ACHY03_001583"
     CDS             complement(3170..4381)
                     /gene="mhpT"
                     /locus_tag="ACHY03_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFS3904828.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLTSLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(5063..6076)
                     /gene="mhpE"
                     /locus_tag="ACHY03_001584"
     CDS             complement(5063..6076)
                     /gene="mhpE"
                     /locus_tag="ACHY03_001584"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFS3904829.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(6073..7023)
                     /gene="mhpF"
                     /locus_tag="ACHY03_001585"
     CDS             complement(6073..7023)
                     /gene="mhpF"
                     /locus_tag="ACHY03_001585"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFS3904830.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLDANVEQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQTYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(7020..7829)
                     /gene="mhpD"
                     /locus_tag="ACHY03_001586"
     CDS             complement(7020..7829)
                     /gene="mhpD"
                     /locus_tag="ACHY03_001586"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFS3904831.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(7839..8705)
                     /gene="mhpC"
                     /locus_tag="ACHY03_001587"
     CDS             complement(7839..8705)
                     /gene="mhpC"
                     /locus_tag="ACHY03_001587"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFS3904832.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LEIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLETNPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            complement(8723..9667)
                     /gene="mhpB"
                     /locus_tag="ACHY03_001588"
     CDS             complement(8723..9667)
                     /gene="mhpB"
                     /locus_tag="ACHY03_001588"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFS3904833.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(9669..11333)
                     /gene="mhpA"
                     /locus_tag="ACHY03_001589"
     CDS             complement(9669..11333)
                     /gene="mhpA"
                     /locus_tag="ACHY03_001589"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFS3904834.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            11524..12357
                     /gene="mhpR"
                     /locus_tag="ACHY03_001590"
     CDS             11524..12357
                     /gene="mhpR"
                     /locus_tag="ACHY03_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFS3904835.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEDKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            12472..13248
                     /locus_tag="ACHY03_001591"
     CDS             12472..13248
                     /locus_tag="ACHY03_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFS3904836.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKLIYVQNEQDAESLHKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEVTMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIVA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            13268..14221
                     /locus_tag="ACHY03_001592"
     CDS             13268..14221
                     /locus_tag="ACHY03_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HFS3904837.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKHFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLEALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            14258..15340
                     /gene="lacI"
                     /locus_tag="ACHY03_001593"
     CDS             14258..15340
                     /gene="lacI"
                     /locus_tag="ACHY03_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFS3904838.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            15463..18537
                     /gene="lacZ"
                     /locus_tag="ACHY03_001594"
     CDS             15463..18537
                     /gene="lacZ"
                     /locus_tag="ACHY03_001594"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFS3904839.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPVADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPMNVAPQGKQVIE
                     LPELPQPESTGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAANLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            18589..19842
                     /gene="lacY"
                     /locus_tag="ACHY03_001595"
     CDS             18589..19842
                     /gene="lacY"
                     /locus_tag="ACHY03_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFS3904840.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            19908..20519
                     /gene="lacA"
                     /locus_tag="ACHY03_001596"
     CDS             19908..20519
                     /gene="lacA"
                     /locus_tag="ACHY03_001596"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFS3904841.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(20622..21803)
                     /gene="cynX"
                     /locus_tag="ACHY03_001597"
     CDS             complement(20622..21803)
                     /gene="cynX"
                     /locus_tag="ACHY03_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFS3904842.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLLLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWTFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(21809..22279)
                     /gene="cynS"
                     /locus_tag="ACHY03_001598"
     CDS             complement(21809..22279)
                     /gene="cynS"
                     /locus_tag="ACHY03_001598"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFS3904843.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(22310..22969)
                     /gene="cynT"
                     /locus_tag="ACHY03_001599"
     CDS             complement(22310..22969)
                     /gene="cynT"
                     /locus_tag="ACHY03_001599"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFS3904844.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLSNL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23079..23978
                     /gene="cynR"
                     /locus_tag="ACHY03_001600"
     CDS             23079..23978
                     /gene="cynR"
                     /locus_tag="ACHY03_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFS3904845.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDMLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(23980..24071)
                     /locus_tag="ACHY03_001601"
                     /pseudo
     CDS             complement(23980..24071)
                     /locus_tag="ACHY03_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24111..25394)
                     /gene="codA"
                     /locus_tag="ACHY03_001602"
     CDS             complement(24111..25394)
                     /gene="codA"
                     /locus_tag="ACHY03_001602"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFS3904846.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVHYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(25384..26643)
                     /gene="codB"
                     /locus_tag="ACHY03_001603"
     CDS             complement(25384..26643)
                     /gene="codB"
                     /locus_tag="ACHY03_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFS3904847.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         6381 a         7405 c         7016 g         6050 t
ORIGIN      
        1 gtattgacat atagcatacc cccctatagt atattgcgtg cagatgatga ggtgcgaaat
       61 gcccagtact ccggaagaga agaaaaaggt ccttactcga gttcgtcgta ttcgggggca
      121 gattgatgct ctggaacggt cgctggaggg tgatgccgaa tgccgtgcca tactccaaca
      181 gatcgctgcc gttcggggcg cggctaatgg gctgatggca gaagtgcttg aaagccatat
      241 ccgggaaacg tttgaccgaa atgactgcta cagccgcgaa gtcagccaat ccgttgacga
      301 cactattgaa ctggttcgtg cctatcttaa atagctgaat ctattaccat attgaggaag
      361 agcgagagat gaaatcacgt gctgccgttg catttgctcc cggtaaaccg ctggaaatcg
      421 ttgaaattga cgttgcacca ccgaaaaaag gtgaagtgct gattaaagtc acccataccg
      481 gcgtttgcca taccgacgca tttaccctct ccggcgatga cccggaaggt gtattcccgg
      541 tggttctcgg tcacgaaggg gctggcgttg tggttgaagt cggtgaaggc gtaaccagcg
      601 tcaaaccagg cgaccatgtg atcccgcttt acaccgcaga gtgcggcgag tgtgagttct
      661 gtcgttctgg caaaactaac ctctgtgttg cggttcgcga aacccagggt aaaggcctga
      721 tgccagacgg caccacccgt ttttcttaca acgggcagcc gctttatcac tacatggggt
      781 gctctacatt cagtgaatac accgtagtcg cggaagtgtc tctggcaaaa attaatccag
      841 aagcaaacca tgaacacgtc tgcctgctgg gctgtggcgt gaccaccggt attggcgcgg
      901 tacacaacac agctaaagtc cagccagggg attctgttgc tgtgtttggt cttggcgcga
      961 ttggtctggc agtggttcag ggcgcgcgtc aggcgaaagc gggacggatt atcgctatcg
     1021 ataccaaccc gaagaaattc gatctggcgc gtcgcttcgg tgccaccgac tgcattaacc
     1081 cgaatgacta cgacaaaccg attaaagatg tcctgctgga tatcaacaaa tggggtatcg
     1141 accatacctt tgaatgcatc ggtaatgtca acgtgatgcg tgcggcgctg gaaagtgcgc
     1201 accgcggctg gggtcagtcg gtgatcatcg gggtcgcggg ttccggtcag gaaatctcca
     1261 cccgtccatt ccagttggtc actggtcgcg tatggaaagg ttccgcgttt ggcggcgtga
     1321 aaggtcgttc ccagttaccg ggtatggttg aagatgcgat gaaaggtgat atcgatctgg
     1381 aaccgtttgt cacgcatacc atgagcctgg atgaaattaa tgacgccttc gacctgatgc
     1441 atgaaggcaa atccattcga accgtaattc gttactgatt tcccgctggt ttaccccgtc
     1501 cacttcagac ggggttctta atactctccc cgggcagccg tccgggggat taaccgtgag
     1561 ataatgactg atggaactca ttgaaaaaca tgccagcttt ggcggctggc aaaatgtgta
     1621 tcggcattat tccgcatcac tgaaatgtga aatgaatgtc ggcgtctatc tcccaccaaa
     1681 agccgcgaat gaaaaattgc cggtgctgta ctggctttca ggcctgacct gcaacgagca
     1741 gaatttcatt actaaatcgg ggatgcagcg ttacgcggct gagcataaca ttattgttgt
     1801 tgcgccggac accagtccgc gaggcagtca tgtcgcagat gctgaccgtt acgatctcgg
     1861 gcaaggtgcc gggttttacc tgaacgcgac gcaagcgccg tggaatgaac attacaaaat
     1921 gtatgactat atccgcaacg aactgccgga tttagtgatg catcattttc cggcaacgac
     1981 caaaaagtct atctctggtc attctatggg cgggctgggc gcgctggtgc tggcgttacg
     2041 taacccagat gaatatgtca gcgtctcggc gttttcgccc attgtctccc catcgcaagt
     2101 accgtgggga cagcaagcct ttgccgcata tcttggtgaa aataaagagg cctggttgga
     2161 ttacgacccg gtgagtctta tttcacaagg tcaacgcgtt gcagaaatca tggttgatca
     2221 ggggttgagt gatgattttt acgcggaaca gctgcggact caaaatcttg aaaagatctg
     2281 ccaggagatg aatatcaaga cgttaatccg ttatcacgag ggttacgacc acagctatta
     2341 ttttgtctcc agttttattg gcgagcatat tgcctaccac gccaataaac tgaatatgcg
     2401 ttgataatag tgcacgactg ccggatgcgg cgtgaacgcc ttatccgcct acatttcgcc
     2461 cgcaggccgt aggcatgata agacgcgcat agcgtcgcat caggcatttg cgcacgactt
     2521 atctatgttt accacatcaa atcatcaggc actttgaagt cggcatacgg atcgtcttca
     2581 tcctgctctt cggcactaag cgcgctgtgc aacacaatac tgctggcatc acgctgagca
     2641 attttatcgg caacgctggc gggaataatg gcgtattcac cttcgctatt gttatcgacc
     2701 aacaagcggg caatcgccag acgaccatta atcaactgcg cctgtgtgag cttatcgaca
     2761 aaaatcttct taatcagatt gccgtcagtg aagttaaaac caatatcgcc attggcaatg
     2821 gtgatccggt tcatttcaat aagctgcttc acctgagctt tatattcttt cgccaacgcc
     2881 gcttgttttt gctgttcgct aagctgttta tcacgctcaa gctgtgcctt tttattttct
     2941 tctaccgccg cccgagcttc acgcgcctga acacgcgatt ttttcgccgt tctctcgacc
     3001 ttcgccgctt ttttgctggt gactaatcct gctttgagca actgctcttg taaggtaagt
     3061 tttgccatct ttgtttccaa acccgctgaa taatttttag cgagtatacc tgtaaacgcc
     3121 gtgggtgtat tgggggcaag tccctttcgc ccctgcttaa taaggcgcgt caggcatcgg
     3181 cacacggctg cattcttgat ctccgactca tcaaaataaa caccgccaac ccagcaacaa
     3241 gaatacccgg tgcagaagcg gccattacgc cgaccgtgcc agtgcctaat gccagcattt
     3301 tcccggccag taacggaccg ctcatagccc ccagacgccc taccgccacg gctgttccca
     3361 cacctgttgc gcggatctgc gaactgtaaa acaacggtgc cagggcatac aaaacgcttt
     3421 gcccacctgt cgcaaacaac cccgcgacaa atccggccag caacataccg ttaaatgacg
     3481 acaccgttcc aagcgccagc agcgaagtta acatgccgct ataaatcagt agcgacatgg
     3541 ttactggacg cagcttatcc atcaatgcgc ccaacattaa cgtcccgctc gccgcaccca
     3601 tttgcagtgc aaacatcacc cctgccgcct gcgatggctg gaatccttgc tccaccaaaa
     3661 gcagcggtag ccagttgatc aacatgtaaa ccaccagcag agtgaagaaa taacacagcc
     3721 acagcagcag cgtcgcggtt gctgtttctg gcgcaaataa cgcacgcagt ggtggcgcag
     3781 cctgtttttc gccagcgaaa accgccgact ccggcaacca gcgcattaat agcggcacca
     3841 gaatcaacgg caccacacca cctacccaaa acaccgtttg ccatgctaag tttgcccccg
     3901 cgaaacccag tgtcgccgcc agcgccgcgc caatgggaac accgcaatac atcaggctca
     3961 ctgccgtccc acgaaaacgt ggacccgcgg cttcagacgt cagggcgata agattcggca
     4021 acgccgcccc cagcccgaca ccggtcatca gccgcgcaaa gaccagtgag gggaaatccc
     4081 aggcaatcgc cgttgccagt gagaacaaac caaacagcgc aactgagcca atcaaaatac
     4141 gcttacgacc ataacggtcc gccagcattc cgccaaccaa cgcgccgggt agcaaaccga
     4201 gtattccggc gctgaatatc cagcccattt gcattttatc gagtgcgaaa gcctgggcga
     4261 tgccacccgc cgcaatgcca gccgcctgaa gatccagccc ttccatcaga gcgaccaaaa
     4321 aacaaagccc gatagtcagc atcaggcggg atgaagatga tgaaggggta cgagtcgaca
     4381 taagatgaac ctcgttgcga gtgcaggtga atcgcgactg atacattgcc agccgcgtaa
     4441 tcagaactat ttaatgactg cctacgggcg aaatgtaggc cggataaggc gttcacgccg
     4501 catccggcag tcgtgtgcag atgcctgatg cgacgcttgc gcgtcttatc aggcctacaa
     4561 attcccgcgc catccgtagg ccggataagg tgtttacgcc gcatccggca gtcgtgcgca
     4621 gatgcctgat gcgacgcttg cgcgtcttat caggcctaca aattcccgcg ccatccgtag
     4681 gccggataag gcgtttacgc cgcatccggc agtcgtgtgc agatgcctga tgcgacgctt
     4741 gcgcgtctta tcaggcctac aaattctcgc gccatccgta ggccggataa ggcgttcacg
     4801 ccgcatccgg cagtcgtgcg cagatgcctg atgcgacgct aacgcgtctt atcaggccta
     4861 caaattctcg cgccatccgt aggccggata aggcgtttac gccgcatccg gcagtcgtgc
     4921 gcagatgcct gatgcgacgc taacgcgtct tatcaggcct acaaattctc gcgccatccg
     4981 taggccggat aaggcgttca cgccgcatcc ggcagtcgtg ctgggatgcg catacaaaat
     5041 gcacgttctc gtgcagtcaa tattatttgt tgttgcgcag atccagcgcc acgtcgacga
     5101 tcatatcctc ctggccgcca accatccggc gtttgcccag ctcaacgaga atatccaccg
     5161 cgcttaagcc ataacgcgcc gccgccgttt cacagtgacg caggaagctg gagtaaacac
     5221 cagcgtatcc cagcgccagc gtttcgcgat cgactcgtac cggtcgatcc tgcaacggac
     5281 gcaccaggtc gtcagcggca tccattaacg catagagatc ggccccatgc tgccaaccca
     5341 gtttatccgc ggcggcaata aatacttcca gcggcgcgtt acctgcgccc gcgcccattc
     5401 ccgcgaggct ggcgtcgatt cggtcgcagc cctcttccac cgccgcgata gagttcgcca
     5461 cgccaagact caggttatgg tgagcgtgca tgccagtttg cgtttctggt ttcagcactg
     5521 ctttcagggc gcggaaacgg tcacggatat cgctcatgtt catcgcaccg ccagaatcca
     5581 ccacataaat acaggtcgca ccgtagcctt ccatcagctt tgcctgcttg gcgagattct
     5641 ccggcgtggt catatggctc atcatcagaa aaccaacggt gtccattccg agctcgcggg
     5701 catactgaat atgctgggcg gaaacatcag cttcggtaca gtgcgtagcc acacgaacca
     5761 cccgcgcgcc agcctgccag gcatttttca gatcgtgaat agtgccgatt cctggcagca
     5821 acaacgtcgc gattttggcg tgcttcacca catccgccgc tgcttcaatc cattcaaggt
     5881 cgctatgtgc gccgaaacca tagttaaagc tggacccctg caaaccgtcg ccgtgggcca
     5941 cttcaatcga atccacgcgg gcatcgtcca gtgctttggc aatctggcga acgttttcca
     6001 gcgaatactg atgacgaatg gcgtgcatac cgtcacgcaa cgtgacgtcc gagatataaa
     6061 gttttttacc gttcatgccg cttctcctgc cttgcgcgcc agtgactggg ccattttttc
     6121 cgctgtcgcc agcgcactgg aagtcataat gtcgaggttg cccgcatagg caggcagata
     6181 atgcgctgcg ccttcgactt ccagccagac cgctgttttc agtccggaga attgccccac
     6241 gcccggtaaa ttgaccggtt tatcctgcgg gataacttca aactgcacgc gctgtttcag
     6301 gcgatatccc ggtacgtaag tctgcaccgc ctcagccatt tcattgattg aggcttcgat
     6361 atcatcttgt gaagcttcgt cgctcaatac atacaccgtg tcacgcatca tcaacggtgg
     6421 ctctgctggg ttaagcacga taattgcctt cccttttgcc gcgccgccca ccacttcaat
     6481 ggctcgggaa gtggtttccg taaattcatc gatattggca cgcgtgccag gtccggcaga
     6541 tttactggcg atagaagcga taatttcggc gtaatgaaca cgcgccacgc gtgaaactgc
     6601 cgccaccatt ggaatggtgg cctggccgcc gcaggtgacc atattgacgt tcagttgttc
     6661 gacattcgca tcaaggttaa ccaccggcac gcagtaaggg ccgatggcag caggcgtcag
     6721 gtcgattaag cgaatatccg gtttcgcttc gcgcaaagcg gcatcgtttt tcacatgtgc
     6781 gcccgcgctg gtcgcatcaa atacaatgtc gatatcagca aattcaggca tgttcatcag
     6841 cccgatcacc ccttcatggg tggtggcgac gcccatacgt ctggcacgcg ccagaccgtc
     6901 ggactgagga tcaataccga ccatcaccgc catctccaga tgctgaccgt gacgcaaaat
     6961 tttaatcatc agatcggtgc caatgttgcc agaaccgata atggcgactt tacgcttact
     7021 catgacagac ttccttttgg ggctgcgctt gaaaatgtcg cagcaactga acctatgcct
     7081 tcaatatggg cttcaaaacg atcgcccgca ttcactgcca ccatcggacc taatgccccg
     7141 gtaagaatga tatctccggc gcgcagcggt tcacccagac tggccatttt gcgtgccagc
     7201 cagacggcgg cattaagcgg atgtcccagg cattcgctgc cgcgcccgct ggaaacctct
     7261 tcgttattac gcgtcatctt catggcgcag tttttcaggt ctaaccccgc cggacgttgc
     7321 gccggaccgc cgatgacata caccccgcag gaagcgttat ctgccacggt atcgacaaac
     7381 tgaatcgacc agtcgcgaat gcggctaccc accacctcca gcgccggaag tacccattca
     7441 atggcgttat acaattcgtc gaaggtgata tcagttgcgg gtaaatcgcg gttcaacacc
     7501 agtgcgatct ccgcttcaat gcggggttgc agaacacggg aaaaaggaat gatttcgtta
     7561 tcgccataac acatgtcggc aaataatgtc ccgaaatccg gttgatcaac gcccagttgt
     7621 tgctgcactt tcggatgtgt caggcccact ttacgcccta ccacgcgacg cccctgcgca
     7681 acgtcatatt gcacatttat gtgctgaatg gcgtaggccg cttcagcgtt atcgatacca
     7741 atcagatcgc gcagcggcgc aatcgcttcg ccctgctctg cggcgcggcg taaatccgcc
     7801 gccagttgct caagagtatg cttcgtcatg accgttcctt aagcacgtgc gaggaaattc
     7861 agcaccagtt gattgaaagc gtcggcatgt tcccactgcg cccagtgacc acagtcgcgg
     7921 aagatatgca gttcagaacc ggcaatgccg gacagcagac gcagacccgc atccatcggc
     7981 acaaagcggt cgttgcgccc ccagacaatc agggtttgcg ctttgatttc cgccagacgt
     8041 gggccaaaat ccgggaactg tttcggatta gtttccaggc tcttaacgaa attttccagg
     8101 tgatcgcggc gcgacagcat attattcagg cgcgcttcaa acagggcgtc ggtcaaatcg
     8161 ctggtatcaa aaacgaagat atccatcatc agcttcaggt tttcgatagt cggctgacga
     8221 taaagctgat tcagtcgctt aataccttcg gttggcatcg gcgtaaacaa actcatgccg
     8281 cccgtaccgc cgcccatcag caccagtttg ccgacgcgct ccggccagtt aagggtgaac
     8341 gccacagaac tatgaccacc catcgagttg cccagcaggt ggattttggc gatttccagt
     8401 tgatccacca cgcttttcag gattcgtgca ttaagatccg atcgcgaacc actattaacg
     8461 atcgaatcgc tcttgcccca acccggacaa tccagcagga tcacccgata gcccgcctct
     8521 accagcggat cgatattgcg gctgaagttc gcccagccag tagcacccgg gccggaacca
     8581 tgcagcagga caacggtttc gtcgccttgt ccgcagtcat taaaatggat gcgcagcgtt
     8641 ttacccgctt cttctacatt cagaaaacgg ctggtggcgg cttcggtttg tggctgataa
     8701 ctcatcatct tctcctgcat attcagttct ctgttctggc gcttaacgag ccaaatccgg
     8761 caatccactc cgggattggg cggtaataac gcccttcgct acgccagttg ccaaatgcag
     8821 aaatagcggc aaaagcggcg acccaggttt tgatttcatg tgtcgacttt ccggcaatgg
     8881 cggaaagctc ttcgttactg acggcatcca gttcctgtat gcgtccctgc tccagcaaag
     8941 tcatgaactg gttatcccaa atcgggttga gcggatgcag agttctctga tcctcaacaa
     9001 acttctcagc ggcgctaatc acccgttgct gacgcaattc gcgctcactg gcgggtaaat
     9061 ctttcccgct ccccaacagg cggtcgcgca tatgggcatc ggctttcgcc agttcgggca
     9121 ccggcggctg atgggaaagc ccaccggaac ccaggaacag cacgcgttta ttgagagtgc
     9181 tggtgaaacg tccaatggct tcacccaaca tgcgggtacg ctggaaaccg ggcagcggcg
     9241 tggcgacacc gttgatgaac acaggcagaa ctggcacctt atccagccca ccgagcagga
     9301 actccagcgg ctgggcgaac ccgtggtcca cctgcataca gtaagaaacg gcaagatcga
     9361 tcccgctctt catgacggca tgcgcacagg cctccgccag ctccacaggc acgggcagct
     9421 ctcctgccgc actgccgaaa tcaccaattg ccgtcgctcc aacgcctaaa cagaacggtg
     9481 gcatcacgtc atagaaaaag ccgttgtagt gatctggcgc aaacagcacc accagttcag
     9541 gggagaaagc cgcaatacgc tcgcgggcgc tggcaatcac gccattgacc tcatcgagta
     9601 cctcttgtgc cgggtcgaca taccccacca gcggcgagtg ggaaagacaa tgaagataag
     9661 cgtgcatatc aggctacctt ttcgacagaa acgtcggcat cagggcgggt cagcgtcatc
     9721 accgacgcca gtttattcag ggtcttgccc agagtttgcg gaatggcggt ggcggcaaca
     9781 aagcgatccg ggcgcatcac caccagcgaa gcattgtgtt gcgcgaacca gctacgcagg
     9841 cgaccttgcg tatcccccac gcgtagtacg ccgtcgtggt tatcctgtgc ggtatgaatt
     9901 tgcacttccg gcaccacctg aatgaagcgt gtgcccaacg cgcgccactg ctggatttgc
     9961 tcgtcgctca tcccccacag tggattgcat ccccagccaa ttaccgcgaa gttcgcgccg
    10021 atcgcgttat cgagcagcgt cacgtcgccg ttttccagcg tgactttcgg ctgaataaac
    10081 atcttgccga ccggagagtg cttcgcttcg ccctcacgta ccagcgcacc gccgtaatat
    10141 tgcggcatcg gcttgaagcg catttcgagg aagtagcgtt ttactggcgg cagataattc
    10201 agcagccagg aaacgccgtc acgtaacgta ccctgccagc gtttcggcgg agccagcacg
    10261 ttgcccgccg tcacggacag gtcaatcatc gctttggcgt gatcgcgacg ttcttgttga
    10321 taggtatcga gcagcgcatc gcgggctttc ccctggataa ccaacgccag tttccatgcg
    10381 aggttaaagg cgtcgcgcat gccactgtta tagccctgcc cctgccatac cggcatgatg
    10441 tgtgcggcat cgcccgccag cagtacgcga tcaatacgga aacgttgcgc cagtcgcgcg
    10501 ttgtgggtgt agacacgctg gcgaatcaat tcaacattgt ccggattagg cagcactttg
    10561 cttaacagct tgcgcatatt ttgcggctca cgcagctgtt cttcggtttc tcctggcatc
    10621 accataaatt caaagcgacg taccgcatga ggtagcgcgg cagaaacata cgggcgcacc
    10681 ggatcgcaac acaaatagat atgcggcgta cttaacggat cgttggcgat atctaccaca
    10741 atccactgat ttggcgcagt tttaccttca aacggcacat tcagggtgcg acggacaaaa
    10801 cttgccccac catcacaggc caccagccac tgggctttga ctatttcccg ctgcccttct
    10861 gccgttttca ggtgcaaggt cacttcgtca tcttgctgac tgaaggcctc cagctcgcgg
    10921 gaaaacaagc agcgcacatt cggaaaacgc gacacccctt ccagcatcac cgcatcgacc
    10981 tgcggctgaa taaaggcgtt acggcgcggc cagccaaatt catcggtcat tggctgaata
    11041 tcagcaaaac agcggccctt cggggtgaga aaacgcatcg cgtgccacgg tgtagtgtgc
    11101 ggcagaacat tatcgaccag gccaaccgac tgcatggtgc gcagcgcctc gtcatcaata
    11161 ccaatcgcac gcgggtagtc gatcaactta tcgagtttct ccaccaccag cacgtcaatg
    11221 cccatctggc cgagatagtt cgccatcatc agcccgaccg gaccggcacc agcgatcgcc
    11281 acctgaacgc tatggttaac agcaggctgg atgtcagggt gttgtattgc catttcagta
    11341 cctcacgact cggacaaaat gccgttgcgc gcacagtaca gcgcaactta ttttgttaaa
    11401 aacatgtaaa tgatttttta ttgtgcgctc agtataggaa gggtgttttc ggctacaatc
    11461 aaaacatgcc cgaatgtgca ccaggtgcac aacgttgttt taactataga aatgtcaatt
    11521 aatatgcaga acaatgagca gacggaatac aaaaccgtgc gcggcttaac ccgcggtcta
    11581 atgttattaa atatgttaaa taaacttgac ggcggtgcca gcgtcgggct gctggcggaa
    11641 ctcagcggcc tgcatcgcac cactgtgcgg cgactgctgg agacgctgca ggaagaggga
    11701 tatgtccggc gtagtccctc cgatgatagt tttcgtctga ccatcaaagt gcggcaatta
    11761 agcgaaggat ttcgtgacga acagtggatt tctgcactgg cggccccact gctgggcgat
    11821 ctgttacgcg aagtggtatg gccgacagat gtgtccacgc tggatgttga tgcaatggtg
    11881 gtacgcgaaa ccacccaccg tttcagccgc ttatcctttc accgggcaat ggtcgggcga
    11941 cgtttgccgc ttctgaaaac cgcctcgggc ctgacctggc tggccttttg cccggaacaa
    12001 gaacgcaagg aattaatcga gatgttagcc tcccgccccg gtgatgacta tcaactggca
    12061 cgagaaccgt taaagctgga agccattctg gcgcgcgcgc gcaaagaggg ttacggacag
    12121 aactaccgcg gctgggatca ggaggataag atcgcctcta tcgccgtacc gctgcgcagt
    12181 gaacaacggg tgattggctg tttgaatctg gtgtatatgg cgagcgcaat gaccatcgaa
    12241 caggcagcgg aaaagcatct tccggcacta caacgggtag caaaacagat cgaagaaggg
    12301 gttgaatcgc aggctattct ggtggccgga aggcgaagcg gcgtgcattt acgctagtta
    12361 cgccaatgtg taatttggcc cattctcatc gttattcgcc catttgccca catgcacagt
    12421 acgggctgca cgctacgcta atgacatcaa tactgtgatg tggagcaaat catgaaaatt
    12481 acgcaaattc gtaatgccac ccaaattatc caatacgctg gcaaaacatt tcttatcgac
    12541 ccgttactcg cgcctaaagg aagttatcct ggttttgccg gaacggcacg cgcagaaatc
    12601 cgcaatccaa cggtagaatt accgtgcgat ttaacaacgt tactcaacgt tgacgcccta
    12661 atcgtgaccc atttgcatga agatcactgg gacgaaacag cctcccgcgt cgtccctaaa
    12721 gataaactga tttatgtgca aaatgagcaa gatgctgaat cactgcataa acaaggattt
    12781 acacatctta ccgtacttac cgatgagact aaatttggcg atattactct gcataaaacg
    12841 acagggcagc acggctccga tcgcacctat gccgacccgg cgatggcgaa gcgtttaggc
    12901 gcagcctgcg gcgttgtttt ccagcacccg acagagaaaa cgctctatct ggctggcgat
    12961 accctctggc gtgacgacgt cgaggttaca atgctaactt tccaaccaga agtggttgtg
    13021 ttgaatgccg gatttgcgca tgttctgggg catggcgcga taattatggg agcagaagat
    13081 attctcaaaa cacattttac cctgccagaa gcgcaaattg tcgcgacaca tatggaggcc
    13141 gtaaaccact gtttgctgac gcgtgatgcg ctcaaggaat acgccagaga taatcaaatt
    13201 atcgaatttg tgaacgtacc agaagatggt gaaacgttga cattctaatt cggagaaaaa
    13261 gagtgttatg cgcccgctaa ctgttgccat tatcgccgtt gccggtttta gtccttttca
    13321 cctttccgta ccgtttatcg tatttagcga aaaaatggcg gagaaaaaac actttcacgt
    13381 aattatctgc gctgaaaagc cgggaaacgt ggactctgcc gatgggtttt ccgtaaccgc
    13441 cacccatgac tataccgcag tcatccaggc ggatattgtg ataattcctt actggggaac
    13501 aattacacaa aaaccgccac aaaaactgct ggaagcctta acgaccgcac gggataacgg
    13561 cgcacagatt gtcgggcttt gcctgggcac gtttgtgctc ggctatgcag gtttactgaa
    13621 aaataagcgt gccgccacac actgggagtt cgagcgtgaa tttcaggcac gttttccaca
    13681 aacacatctg gatattaacg cgttgtacgt agacgatgac ggcattatta cctctgccgg
    13741 tactgccgcg gcgctggatt gctgtttgta tattgttcgg caacattttg gcagcgacta
    13801 tgctaaccat attgcccgac ggatggtcgt accgccatat cgcaccggcg gtcaggcgca
    13861 gtttattgag caaccagtgc cgaaaaatac ccatgatgaa cgcattaatc tcctgttgga
    13921 ttacctgcgg caaaacattg cgcaacagca tgatctcgac tcgctggcgc agcgagtaat
    13981 gatgagtcgc cgcacattaa ctcgccattt tatgaaagcg accggttcga gtatcgccga
    14041 atggcttatt actgaacgct tacgccgtag ccaggaactg ttgggatcca gtcaattgcc
    14101 cgttgagcgg atagcagctg aggtgggttt tctctcacct gtgacctggc gtcagcattt
    14161 taaatctcac ttcggcgtca gccccgccga atggcgcaaa acctttcgcg gtatggcatg
    14221 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
    14281 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga atcaggccag
    14341 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
    14401 tcccaaccgc gtggcacaac aactggcggg gaaacagtcg ttgctgattg gcgttgccac
    14461 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
    14521 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
    14581 agcggcggtg cacaatcttc tcgcacaacg cgtcagtggg ctgatcatta actatccgct
    14641 ggatgaccag gatgccattg ctgtggaagc tgcctgcgct aatgttccgg cgttatttct
    14701 tgatgtctct gaccagactc ccatcaacag tattattttc tcccatgaag acggtacgcg
    14761 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
    14821 attaagttct gtctcggcgc gtctgcgtct ggcgggctgg cataaatatc tcactcgcaa
    14881 tcaaattcag ccgatagcgg aacgggaggg cgactggagt gccatgtccg gttttcaaca
    14941 aaccatgcaa atgctaaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
    15001 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ttgcgcgttg gtgcggatat
    15061 ctcggtagtg ggatacgacg ataccgaaga cagctcgtgt tatatcccgc cgttaaccac
    15121 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
    15181 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
    15241 caccctggcg cccaagacgc aaaccgcttc tccccgcgcg ttggccgatt cattaatgca
    15301 gctggcacga caagtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    15361 gtcagctcac tcattaggca ccccaggctt tacactctat gcgtccggct cgtatgttgt
    15421 gtgaaattgt gagcggataa caatttcaca caggatacag ctatgactat gattacggat
    15481 tcactggccg tcgtattaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    15541 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccaat
    15601 cgcccttccc aacagttgcg cagcctgaat ggtgagtggc aatttgtctg gtttccggca
    15661 ccagaagcgg ttccggaaag ctggctggag tgcgatcttc ctgtcgccga tactgtcgtc
    15721 gtcccctcaa actggcagat gcacggttac gacgcgccca tctacaccaa cgtgacctat
    15781 cccattacgg tcaatccgcc atttgttccc acggagaatc cgacgggttg ttactcgctc
    15841 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
    15901 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagt
    15961 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg
    16021 gtgatggtgc tgcgctggag tgacggcagt tatctggaag atcaggatat gtggcggatg
    16081 agcggcattt tccgtgacgt ctcgttgctg cacaaaccga ccacacaaat cagcgatttc
    16141 catgttgcca ctcgctttaa tgatgatttc agccgcgcgg ttctggaggc agaagttcag
    16201 atgtacggcg agctgcgcga tgagctgcgg gtgacggttt ctttgtggca gggtgaaacg
    16261 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggcggttat
    16321 gccgatcgcg tcacactagg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc
    16381 ccgaatatct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca
    16441 gaagcctgcg atgtcggttt ccgcgaggta cggattgaaa atggtctgct gctgctgaac
    16501 ggcaagccgt tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag
    16561 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
    16621 gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac
    16681 ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
    16741 ctgaccgatg atccgcgctg gctacccgcg atgagcgaac gcgtaacgcg aatggtgcag
    16801 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgagtc aggccacggc
    16861 gctaatcacg acgcactcta tcgctggatt aaatctgtcg atccatcccg cccggtgcag
    16921 tatgaaggcg gcggagccga cacctccgca accgatatta tttgcccgat gtacgcgcgc
    16981 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
    17041 ttgcctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
    17101 cttggcggct tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
    17161 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg
    17221 tcggcttacg gcggtgattt tggcgatacg ccaaacgatc gccagttctg catgaacggt
    17281 ctggtctttg ccgaccgcac gccgcatccg gcgctgacgg aagcaaaaca ccagcagcag
    17341 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
    17401 catagcgata acgagctcct gcactggatg gtggcgctgg atggcaagcc gctggcaagc
    17461 ggtgaagtac ctatgaatgt cgctccacaa ggtaaacagg taattgaact gcctgaacta
    17521 ccgcagccgg agagcaccgg gcaactctgg ctaacggtac acgtagtgca accgaacgcg
    17581 accgcatggt cagaagccgg acacatcagc gcctggcagc agtggcgtct ggcggcaaac
    17641 ctcagcgtga cactcccctc cgcgccccac gccatcccgc aactgaccac cagcgaaacg
    17701 gatttttgca tcgagctgga taataagcgt tggcaattta accgccagtc aggctttctt
    17761 tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
    17821 cgcgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga ccctaacgcc
    17881 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcggcgtt gttgcagtgc
    17941 acggcagata cacttgccga cgcggtgctg attaccactg tccacgcatg gcagcatcag
    18001 ggaaaaacct tatttattag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
    18061 attaccgttg atgttgaagt agcgagcgat acaccgcatc cggcacggat tggcctgacc
    18121 tgccagctgg cgcaggttgc agagcgggta aactggctcg gattagggcc gcaagaaaac
    18181 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
    18241 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gtgggacgcg cgaattgaat
    18301 tatggcccac accagtggcg cggcgacttc cagttcaaca tcagccgcta cagccaacaa
    18361 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
    18421 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtgtcggcg
    18481 gaattccagc ttagcgccgg tagctaccat taccagttgg tctggtgcca aaaataataa
    18541 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
    18601 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatggga
    18661 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
    18721 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
    18781 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
    18841 gtgatgtttg cgccgttctt tatttttatc ttcggcccgc tgttacagta caacatttta
    18901 gtagggtcga ttgttggtgg tatttatctt ggtttctgtt ttaacgccgg tgcgcccgca
    18961 gtagaggcat ttatcgagaa agtcagccgt cgcagtaatt tcgaatttgg tcgcgcgcgg
    19021 atgtttggct gcgttggctg ggcactgtgt gcctcgattg tcggcatcat gttcaccatc
    19081 aataatcagt ttgttttctg gctgggttct ggctgtgcac tcatcctcgc cattttactc
    19141 tttttcgcca aaacggatgc gccctcttcc gccacggttg ccaatgcggt aggtgccaac
    19201 cattcggcat ttagccttaa actggcgctg gaactgttca gacagccaaa actgtggttt
    19261 ttgtcactgt atgttattgg cgtttcctgc acctacgatg tttttgacca acagtttgct
    19321 aatttcttta cttctttctt tgccaccggt gaacagggta cgcgggtatt tggctacgta
    19381 acgacaatgg gcgaattact taacgcctca attatgttct ttgcgccact gatcattaat
    19441 cgcatcggtg ggaaaaatgc cctgctgctg gctggcacta ttatgtctgt acgtattatt
    19501 ggctcatcgt tcgccacctc agcgctggaa gtggttattc tgaaaacgct gcatatgttt
    19561 gaagtaccgt tcctgctggt gggctgcttt aaatatatta ccagccagtt tgaagtgcgt
    19621 ttttcagcga cgatttatct ggtctgtttc tgcttcttta agcaactggc gatgattttt
    19681 atgtctgtac tggcgggtaa tatgtatgaa agcatcggtt tccagggcgc ttatctggtg
    19741 ctgggtctgg tggcgctggg cttcacctta atttccgtgt tcacgcttag cggccccggc
    19801 ccgctttctc tactgcgtcg tcaggtgaat gaagtcgctt aagcaatcaa tgtcggatgc
    19861 ggcgcgagcg ccttatccga ccaacatatc aggacggaat gatcgcaatg aacatgtcga
    19921 tgacagaaag aataaaagca ggcaagctat ttaccgatat gtgcgaaggc ttacctgaaa
    19981 aaagacttcg tgggaaaacg ttaatgtatg agtttaatca ctcgcatcca tcagaagttg
    20041 aaaaaagaga aagcctgatt aaagaaatgt ttgccacggt aggggaaaac gcctggatag
    20101 aaccacctgt ctatttctct tacggttcca acatccatat aggccgcaat ttttatgcaa
    20161 atttcaattt aaccattgtc gacgactaca cggtaacgat cggtgataac gtattgattg
    20221 cacccaatgt tactctttcc gttacgggac accctgtaca ccatgaattg agaaaaaacg
    20281 gtgagatgta ctcttttccg ataacgattg gcaataacgt ctggatcgga agtcatgtgg
    20341 ttattaatcc aggcgtcacc atcggggata attctgttat tggcgcgggt agtgtcgtca
    20401 taaaagacat tccaccaaac gtcgtggctg ctggcgttcc ttgccgggtt attcgcgaaa
    20461 taaacgaccg ggataagcaa tattatttca gagattacaa agttgaatcg tcagtttaaa
    20521 ttagcaaaat tgcctgatgc gcttcgctta tcaggcctac aaattcagtg ttctacgtta
    20581 gccgcatccg gcatgaacaa agcgcaggaa caagcgtcgc atcatgcctc tttgacccac
    20641 agctgcggaa aacgtgctgg tgcaaaacgc agggttatga tcatcagccc aacgacgcac
    20701 agcgcatgaa acgtccagtc catcaggtaa ttgccgctga tactacgcag cacgccagaa
    20761 aaccacgggg caagcccggc gatgataaaa ccgatcccct gcataaacgc caccagcttg
    20821 ccagcaatag ccggttgcac agagtgatcg agagccagca gcaaacagag cggaaatgcg
    20881 ccgcccagac ctaaaccaca caccatcgcc cacaataccg gcaattgcag cggcagccag
    20941 ataaagccgc agaaacccac cagttgtaac gccagtgcca acagcaaaag tttgcgccga
    21001 tcctgatggc gagccatagc aggcattagc aaagctcctg cggcttgccc aagcgtcatc
    21061 aatgccagca aggaaccgct gtactgcgcg ctggcaccaa tctcaatata gaaagcgggt
    21121 aaccaggcaa tcaggctggc gtaaccaccg ttaatcagac cgaagtaaac acccagcgtc
    21181 cacgcgcgag gagtgaaaac cacgcgaacc ggagtggttg ttgtcttgtg ggaagaggcg
    21241 acctcgcggg tgctttgcca ccaccaggca aagagcgcaa caacggcagg cagcgcccac
    21301 caggcgagtg tttgatacca ggtttcgcta tgttgaacta accagggcgt cagggcggca
    21361 ccaagtccac cgccgcccat cagagccgcg gaccacagcc ccatcaccag tggcgtgcgc
    21421 tgctgaaacc gccgtttaat caccgaaggc atcaccgcct gaatgatgcc gatccccacc
    21481 ccaccaagca gtgcgctgct aagcagcagc gcactttggg ggtaaagctc acgcatcaat
    21541 gcaccgacgg caatcagtaa caaactgatg gcgacgctgc gacgttcgct gacatgctga
    21601 tgaagccagc ttccggccag cgccagcccg cccatggtaa ccaccggcag agcggtcaac
    21661 agggcagcca cgctaaagct cattccgctt gcctggcgca attgcggtag cagtggcccg
    21721 acggaggtga gcagtggtcg catattaaga ccaattagca ccagaaccag cagcatcgtg
    21781 aacgtctttt gatggttacg catggctgtc agaacggttt ggttggcaga tatttaccat
    21841 ctaaggtgat gaccgcacgt tcgccacctt ccgggtccgc cactttctta acgtcgagtt
    21901 tgaagttaat cgcgctgata atgccatcgc caaatttctc atgaaccaac gctttcaggg
    21961 ttgtgccgta cacctgcaac atttcataga aacgatacat cgttgggtcg gttggaatac
    22021 ggtcatcaat gcagccacgc agtgggatca tctgtaacag tagaatggcg tcttcgtcga
    22081 gatccagctt cgccccgacc tggcgggcgg cgtcggcagg aagcgcctgc tgacccagca
    22141 aagccgcggt cacaaaggct tctgccagcc cggtgccgtc ggcaatctcg gcaaatgaga
    22201 gatctttttt agctttgctg agcaaaatgg catcggcaag atcaagacga atattgcggt
    22261 taatttgtga ctgaatcatg gtggatctcc tgatggttta aaaataaggt tacgctgcgg
    22321 tcggttggcg tagcggtatg gcacaaacgc gaggattagc ggccagtggc acaaactggc
    22381 gggttgcgcc gtcaaacgct gcgatgctgc cgctttcaat gtcgtagacc cagccgtgca
    22441 gggcgatccg cccctcttcg agcgccaggc gcaccgatgg atgagtttgc aaattagaca
    22501 actgagcaat gacgttttca cgtaccatcg ccgcagcttt tgacggtaaa tcgggatgcg
    22561 ggcgcgcctc attaacgacg cgggctgaat cggcataacg cagccagtgg gagacggcag
    22621 gcatatggtc catgcactga cagctggcaa tggcggtcat tgcgccacag ttggaatgac
    22681 cgcaaatcac aatgtcagat acccgaagcg cggcgacggc atactccacc gaagcagaaa
    22741 cgccaccggg ttccggcccg taggaaggga cgatattgcc cgcattgcga ataacgaaca
    22801 gatcgccagg ctcacgttgc gtcaccagct cagggaccag acggctgtcg gagcaggaga
    22861 taaaaagtgt gcgcgggctt tgctgtgtcg ccagctgttt aaacaaggcc tcccgcttcg
    22921 gaaatgcctc gcgctggaat ttaaggaatc catcaataat ctctttcacc gttaacctct
    22981 gtctgtctct ggaatgagag gcagactact cgccgattgt cataaggtaa aagtctcatt
    23041 tatgatgagt tctattggat ttacttatag gttggcgaat gctctctcga catatcaatt
    23101 attttcttgc cgtggctgaa catggcagct tcacccgtgc cgccagtgcg ttgcacgtct
    23161 cccagcctgc gctttcccag cagattcgcc agttagagga gagtttaggc gtgccgctgt
    23221 ttgaccgtag cgggcgaacg attcgtctca ctgatgcagg agaagtctgg cgacagtacg
    23281 ccagccgggc gttacaggaa ctgggggcgg gtaaacgggc gattcatgat gttgccgatc
    23341 tgacgcgagg atcgctgcgt atcgccgtca cccccacctt tacgagctac tttatcggcc
    23401 ccttaatggc ggatttctat gcgcgctatc ccggcatcac gctccagcta caggaaatgt
    23461 cgcaggagaa aatcgaggat atgctttgcc gcgacgagtt ggacgttggg attgccttcg
    23521 cgcctgtgca ttcgccggag ctggaggcaa ttcctttact gacagaaagt ttagcgttag
    23581 tcgtggcgca acatcatccg ctggctgcct gtgaacaggt ggcgttgagt cgcttgcatg
    23641 atgaaaaact ggtcctgctc agcgcggaat ttgccaccag agagcaaatt gaccactact
    23701 gcgagaaagc ggggctgcat ccacaggtgg tcattgaggc gaactcaatt agcgcggttc
    23761 tggagctgat tcgccgcact tccctttcca cattgttacc agcagcgatt gccacacaac
    23821 atgacgggct taaagctatt tctcttgccc cgccactact ggagagaacg gcggttttgt
    23881 tgcggcggaa aaatagctgg cagacagccg ccgcgaaggc atttttgcac atggcgttgg
    23941 ataaatgcgc ggttgttggc gggaatgaat cgcggtaacc actgacacaa ttgcctgatg
    24001 cgctacgctt atcaggccta cgagttcagt gctctacgta ggccggataa ggcgttcacg
    24061 ccgcatccgg cataaactac gctcgctttg tcaacacaca gcccagccga tcaccgtttg
    24121 taatcgatgg cttctggctg ctccagatat acggtggttt gtgccggttg tgtgctggca
    24181 atcaccttgc cgccacgtac cgaatagtgc accggaacct gacggcgcag cgcgtcaaat
    24241 ccattttcag ccggcaggat aatcaggttg gcgctgtttc cggcggcaat gccgtaatcc
    24301 tgcaaattca acgttctggc gctgtggtgg gtgattaaat tcaggccatc gttaatctgc
    24361 ccgtagccca tcagctgaca aacatgcagc cccatatgca gcacttgcag catattcgcc
    24421 gttcccagcg gataccacgg atcgaagaca tcatcatgac caaagcagac gttaatgccg
    24481 gactccagca tctctttaac gcgcgtgatg ccgcgacgtt ttggatacgt atcgaaacgt
    24541 ccttgcagat gaatattgac cagcggattg gcgacaaagt taataccgga cattttcagc
    24601 aagcggaaca ggcgtgaggt atacgccccg ttataggagt gcattgccgt ggtgtggctg
    24661 gcggtgactc gcgcgcccat gccttcacgg tgcgccaggg cagcaacggt ttcgacaaag
    24721 cgcgactgct cgtcatcgat ctcatcacag tgaacgtcga tcagacggtc gtatttttgc
    24781 gccagggcga aggttttatg cagcgactcc acgccgtatt cacgggtaaa ttcaaaatgc
    24841 ggaatcgccc ccactacatc tgcccctaag cgtaacgcct cttccagcaa cgcttcaccg
    24901 ttgggatacg acaaaatccc ttcctgaggg aaggcgacga tttgcagatc aatccacggc
    24961 gcgacttcct gcttcacttc cagcattgct ttcagcgcag ttagcgttgc atccgaaaca
    25021 tcgacatggg tacgtacatg ctgaatgccg ttggcaatct gccatttcag cgtttgccat
    25081 gcgcgttgtt tcacatcgtc atgggttaat aacgctttgc gctcggccca gcgttcaatg
    25141 ccttcaaaca gcgtgccgga ctgattccag ttcggttgtc cggcggtttg cgtggtgtcc
    25201 aggtgaatat gtggctccac aaacggcggt ataactaaac cttgttcggc atccaggctg
    25261 ttttcagtta tgggcatcac gccggattgc gcatcaatgg cgctgatttt tccgtcctgc
    25321 agatgaatct gccacagccc ctctttgcct ggtaaccggg cattaataat tgtttgtaaa
    25381 gcgttattcg acactgttag cctccacatg cgtcattgct gctgtcgttt tacgattcaa
    25441 aatcgggtta aggatcagat agctcagcgc gccacctaat accgcgttga ccggaacaat
    25501 tcccggtaac cagtggcctg cagcaatccc caaggcgacc gccagaatcg ccacccaatt
    25561 gacactcatc atacgcgtgg tcgcaaagtg ctcatagcgg cgacggttca tcagatagtc
    25621 ggcaatgatc acgccaccca ctggaggaat agctgccgaa aggaaggtca accagccgac
    25681 aaaattgtta tacagccata atgcacagac cgtaccgata ataccgttga ttaccgaaag
    25741 ggttttgctc gacatcccgg taatgttggc gaaacctaaa cccgacgcat agagcgcgtt
    25801 atcgttggtg gtccagatat tcagcccgag caccacaatc gcaggcaaca gcaggccctg
    25861 agcaatcatc acatcagaga tatccgccat ccccagtgcc gcagcgcccg ctgcaccgaa
    25921 aataaacatc aacgagttgc cgaggaaaaa ggccaccatc gccaccagca ccgccagttt
    25981 ggcattgcga ccaaagcgga caaagtcagc ggtgagcgtg cccgcactga taaatgaccc
    26041 cacaaccagc gccagcgcga cattgaaatc taacggttgt gcgggaacga ccgcttttaa
    26101 tgcgtccaga ccgcccatgc cgttaacggc cagccacacg gaataaccgc caagacaggc
    26161 gatagccgga accgcaatca ccgaaagaac cgtcagcgcc gaaatgccaa agaagacggt
    26221 gacggtcatc agtaaaccgg aaacggcaat cagcaaatta atatccagcc cggttgcctt
    26281 acccaccgga atggcaaaca tcgccacgcc aacgccaaac cagccaacct gggtgccgcc
    26341 cagcagcagt gaaggcagcc atgagccttt aacaccaaac gagaagcgag caagaagatg
    26401 agtggtcagg ccggtttttg cgccaatata accgagaaat gaagtgtaaa taccgaggag
    26461 aagattaccg atgagaactg cgaggaagaa atcatgatag ctaagaccgg ttccgagagt
    26521 gccgccggtc cacatactgg cggaaaagaa ggttaatccc agcatgacga acgtcaatgc
    26581 caatacccct tttcgcgccg actgcgggac tggcccctgg ctaaagttgt tatcttgcga
    26641 cacgaaattc ctccgtttgc tgtgtgaaac ccaaaaaatc cgccgcattc tattcatctg
    26701 aagataaaaa gcaatcgttt tcgtagggga atatattttt tatatgaatg agtgcgaaaa
    26761 ggtgggggaa aattatcggt gtaaaaacag tgggcaggta tgataagacg cgcctgcgtc
    26821 gcatcaggca ctgagcacgg tgtcggatgc gg
//
LOCUS       DAZUCE010000045        26851 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZUCE010000045 DAZUCE010000000
VERSION     DAZUCE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.045"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            136..342
                     /locus_tag="ACHY03_001604"
     CDS             136..342
                     /locus_tag="ACHY03_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFS3904848.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            443..952
                     /locus_tag="ACHY03_001605"
     CDS             443..952
                     /locus_tag="ACHY03_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFS3904849.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            949..2367
                     /gene="phrB"
                     /locus_tag="ACHY03_001606"
     CDS             949..2367
                     /gene="phrB"
                     /locus_tag="ACHY03_001606"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFS3904850.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVELVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKVVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(2517..3998)
                     /gene="dtpD"
                     /locus_tag="ACHY03_001607"
     CDS             complement(2517..3998)
                     /gene="dtpD"
                     /locus_tag="ACHY03_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFS3904851.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            4269..5012
                     /gene="ybgI"
                     /locus_tag="ACHY03_001608"
     CDS             4269..5012
                     /gene="ybgI"
                     /locus_tag="ACHY03_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFS3904852.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5035..5691
                     /gene="pxpB"
                     /locus_tag="ACHY03_001609"
     CDS             5035..5691
                     /gene="pxpB"
                     /locus_tag="ACHY03_001609"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFS3904853.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5685..6617
                     /gene="pxpC"
                     /locus_tag="ACHY03_001610"
     CDS             5685..6617
                     /gene="pxpC"
                     /locus_tag="ACHY03_001610"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFS3904854.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6607..7341
                     /gene="pxpA"
                     /locus_tag="ACHY03_001611"
     CDS             6607..7341
                     /gene="pxpA"
                     /locus_tag="ACHY03_001611"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFS3904855.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMHACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRNQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLHSTFAEKGIVVAA"
     gene            7377..8168
                     /gene="nei"
                     /locus_tag="ACHY03_001612"
     CDS             7377..8168
                     /gene="nei"
                     /locus_tag="ACHY03_001612"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFS3904856.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTMHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(8165..9211)
                     /locus_tag="ACHY03_001613"
     CDS             complement(8165..9211)
                     /locus_tag="ACHY03_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFS3904857.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLACAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(9360..10421)
                     /locus_tag="ACHY03_001614"
     CDS             complement(9360..10421)
                     /locus_tag="ACHY03_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3904858.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDK
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            complement(10418..11149)
                     /locus_tag="ACHY03_001615"
     CDS             complement(10418..11149)
                     /locus_tag="ACHY03_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3904859.1"
                     /translation="MTFVKGFPLILLVASMCSHGAVQPDRTRIIFNSKDKATSLRVEN
                     RSDKLPYLAYSWIENEKGEKSDEFLVALPPIQRLEPKATSQVRIMKQAATAKLPTDRE
                     SLFYYNLREIPPVPEGSEGHAILQVAVQSRIKLFWRPAALRKKMGDHVEQQLQVSQQN
                     NQLTLKNPTGYYLTIAYLGRDEKGVLPGFKSTMVAPFSSVTTSTGSYSGKQFYLGYMD
                     DYGALRMNTLSCQRQCRLQPVENKK"
     gene            complement(11164..13623)
                     /locus_tag="ACHY03_001616"
     CDS             complement(11164..13623)
                     /locus_tag="ACHY03_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS3904860.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALAAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWLGALSFDVTWADSHFDIQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITPLNLNLYANILHQSWWN
                     ADTSTTANITAGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            complement(13673..14239)
                     /locus_tag="ACHY03_001617"
     CDS             complement(13673..14239)
                     /locus_tag="ACHY03_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3904861.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVVLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            complement(14626..15909)
                     /gene="gltA"
                     /locus_tag="ACHY03_001618"
     CDS             complement(14626..15909)
                     /gene="gltA"
                     /locus_tag="ACHY03_001618"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFS3904862.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(16115..16222)
                     /locus_tag="ACHY03_001619"
     CDS             complement(16115..16222)
                     /locus_tag="ACHY03_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3904863.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16603..17007
                     /gene="sdhC"
                     /locus_tag="ACHY03_001620"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             16603..17007
                     /gene="sdhC"
                     /locus_tag="ACHY03_001620"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFS3904864.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            17001..17348
                     /gene="sdhD"
                     /locus_tag="ACHY03_001621"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             17001..17348
                     /gene="sdhD"
                     /locus_tag="ACHY03_001621"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFS3904865.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            17348..19114
                     /gene="sdhA"
                     /locus_tag="ACHY03_001622"
     CDS             17348..19114
                     /gene="sdhA"
                     /locus_tag="ACHY03_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFS3904866.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            19130..19846
                     /gene="sdhB"
                     /locus_tag="ACHY03_001623"
     CDS             19130..19846
                     /gene="sdhB"
                     /locus_tag="ACHY03_001623"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFS3904867.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEADEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNEQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            20147..22948
                     /gene="sucA"
                     /locus_tag="ACHY03_001624"
     CDS             20147..22948
                     /gene="sucA"
                     /locus_tag="ACHY03_001624"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFS3904868.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            22963..24180
                     /gene="odhB"
                     /locus_tag="ACHY03_001625"
     CDS             22963..24180
                     /gene="odhB"
                     /locus_tag="ACHY03_001625"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFS3904869.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAVQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            24456..25622
                     /gene="sucC"
                     /locus_tag="ACHY03_001626"
     CDS             24456..25622
                     /gene="sucC"
                     /locus_tag="ACHY03_001626"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFS3904870.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            25622..26491
                     /gene="sucD"
                     /locus_tag="ACHY03_001627"
     CDS             25622..26491
                     /gene="sucD"
                     /locus_tag="ACHY03_001627"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFS3904871.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         6514 a         6704 c         7304 g         6329 t
ORIGIN      
        1 taaaccatca ataagcaaaa ataagtggtc ggatgagtag taaaattaca caaaaggcgg
       61 tactattttc atcagataaa caaattcatt tttccggtgc tattcaccgg gtagatacaa
      121 aagggggaga aaagtatgga actctacaga gaatatcctg catggcttat ctttttacgc
      181 cgtacttatg cggttgcagc gggcgttctg gcgctgcctt tcatgctttt ctggaaagac
      241 cgcgcccgct tttacagcta cctgcatcgc gtctggtcga aaaccagcga taaaccggtt
      301 tggatggatc aggccgaaaa ggcaaccggt gatttttact gattgctgag ttttgaatac
      361 attgaaaacc gctagtggaa acgctggcgg ttttttcttt tcgccgtcta cacttactcc
      421 tgtaaaccgt tcaggagcag taatgaatca gcaacgattt gatgacagca ccctaatccg
      481 tatcttcgcc cttcatgagt tacatcgact gaaagaacat ggcttaacgc gcggggcgct
      541 tctcgattat cacagccgct ataaactcgt ctttctggct cattcccagc cggaataccg
      601 caaacttggc ccgttcgtgg ctgatattca ccagtggcaa aatctggatg actattataa
      661 ccagtaccgc caacgcgtag ttgttttgct ttctcacccc gccaaccgac gtgaccacac
      721 caacgttttg atgcacgttc agggttattt tcgcccgcat attgattcca cagaacgcca
      781 gcagctggca gcgcttatcg acagttatcg ccgtggcgag caaccgcttc ttgcgccgct
      841 gatgcgtatc aaacactata tggcgcttta tcctgacgcc tggctttcag ggcagcgtta
      901 tttcgaactt tggccgcgtg tgattaactt gcgccattca ggagttttat gactacccat
      961 ctggtctggt ttcgccagga tttacgtctg cacgataatc tcgcgctggc tgccgcctgc
     1021 cgcaattcgt ctgcacgcgt gctggcgttg tatatcgcta caccacgcca gtgggcggcg
     1081 cataatatgt cgccgcgtca ggctgaactt atcaatgctc aactgaacgg gctacaaatc
     1141 gcgctggcgg aaaaaggtat tcctttattg ttccgtgaag tggatgactt tgtcgccagc
     1201 gtcgaattag ttaaacaggt gtgcgcggaa aacagcgtta ctcatctttt ttataactat
     1261 cagtatgagg tgaatgagcg ggcgcgggat gtggacgttg aaagagcgct gcgtaacgtg
     1321 gtgtgtgaag gatttgatga cagcgtgatc ctgccgcctg gcgcggtgat gactggcaat
     1381 cacgagatgt acaaagtctt tacgcctttt aagaatgcct ggctgaaacg gctgcgggaa
     1441 gggatgccgg agtgcgtcgc tgcaccaaaa gttcgtagta gcggatcgat aaagcccgcg
     1501 ccatccatta cgctgaatta tcctcgtcag tctttcgata ctgcgcattt cccggtggaa
     1561 gaaaaagcgg cgattgcgca attacgccag ttttgccaga acggtgccgg agagtatgag
     1621 caacaacgag attttccggc agtggaaggc accagtcgtt tgtccgccag cctggcaacg
     1681 ggcgggttat cgcctcgcca gtgtttgcat cgcttgttgg cggaacagcc gcaggcgctg
     1741 gacggtgggg ccggtagtgt ctggcttagt gagctgatct ggcgcgagtt ctaccgtcat
     1801 ctgatgacgt attacccctc gttgtgtaaa cattgtccgt ttattgcctg gacggatcgt
     1861 gtgcagtggc agagcaatcc cgcacattta caggcctggc aggaaggcaa aacgggatac
     1921 ccgattgtcg atgctgccat gcgtcagctt aacagcactg gctggatgca taacaggcta
     1981 cggatgatta cagccagttt tctggtgaaa gatttattga tcgactggcg cgaaggcgag
     2041 cgatatttca tgtcgcagct gattgatggt gatttggcag ctaataacgg tggctggcag
     2101 tgggcggctt ctacgggtac tgatgcagcg ccgtattttc gtattttcaa cccgacaacc
     2161 cagggcgaga aatttgaccg cgaaggcgag tttatccgcc agtggctacc ggaactgcgc
     2221 gatgtgccag ggaaagtggt gcatgagccg tggaagtggg ctgagaaagc aggtgtgaag
     2281 ctggattatc cgcaaccgat agtcgagcac aaagaggcga gagtacaaac gttggcagcg
     2341 tatgaggcgg cgcggaaggg gaaataacag acgcgtcagg caatcgagcc cagatgccgg
     2401 atgcggcgtg aacgccttat cctgcctaca aatttgtgca aattcaataa attgcaggaa
     2461 atacgtaggc ctgataagac gcgtcaagcg tcgcatcagg catcggtgct ggcctgttaa
     2521 gattccagcg ccagcgcgcg gtttctgaat ttcagcgcct gatacagcca aatcattagt
     2581 accactccta cacacgccag tgcgccccag gtaatttgat caaatacttc aatatatgca
     2641 ttgatggagt agttgatcgc cccggaagca tcaaacgaag cctgcgatgt ctgatcggca
     2701 attacgcccg ccagatagtt cgcaatcgcg ccggaaagca gcatataaat gccggttaat
     2761 acgccggtca caccggggat ttcaatgcgc gtaatctgcg acatagcaac cgggtcgata
     2821 aacagttccg caaagcccat caccgccagg cctaatacca tcagtggcag agaagagtga
     2881 ccatacattg cggaccagcg ggcgcttaag gtcagaatgc agaatccggc gctcatcagg
     2941 ccaaggccaa gagcaaattt tccccagatg cgcacggtac gattacctgc gacgctttct
     3001 ttaaccaccc acgccaggaa cacaccgcac agcataactg cgaaggcatt aatcgactgg
     3061 aacatcgcgg tcggaacggt ataaccaaac atatcgcggt taacgaagcg gtcgatataa
     3121 aggctaatcg agctgcctcc ctgttgtgcg aaggcccaga acaacatact gaagaaggtg
     3181 agcgtcacaa tcagccccag ctccttacgc tgtttctggt tttctgcttt gcgataaatt
     3241 tttgccagta cacccaggcc aataatagtc gcgacaatca aggcgtatac cgaccattct
     3301 ttccagaaca gtacggtaat cagcgcaggc gttgccacca gcagaaccag cagccatccc
     3361 cagttcggca gcagaaagtt tgtcgcacgc agtacttttt tgttaacgcc gcgggtatgt
     3421 gtgaaatgac gattgccaca taagaaaata accagacccg cgatcatacc caccgccgcc
     3481 aggccaaagc ccatcgccca actgtactct tcctgggcgt aaccacaggc gataggtgca
     3541 ataatagacc ccacgttacc cgccgcatac atcagcgaga aaccgccatc acgacgcgga
     3601 tcggttggct catacagctc gccgagcagg caactgacat tggatttaaa cagaccatag
     3661 ccacaaacga ttatcgccag ggacagatag aggaatgacg gatggatctc acttgcaccc
     3721 aacaccacat gaccgatcgc catcaataac gcccccagca tcaccgccat gcgattgccg
     3781 agaactttat ccgccaaaaa gccaccgagg attggcgtga catacaccag cgaacagtag
     3841 gcgctaaata actcataggc gtgagtatcg ttgtatttta gttgattggt gagatagaga
     3901 atcagcaggg cacgcatgcc gtaaaagcta aaatattccc agatttgcag cgcaacgaca
     3961 tagtaaatag cgcgcggttg tgatgcgtgt ttattcataa taattccaca attgacggca
     4021 acgtcacgta atagttgcgc ggcgtgcttg tgtttcctta aagttaaaac tttgatacag
     4081 atctgattat ttatgcaata tgctgtctga ttgcataaat atacattagc tgaagcgtga
     4141 tgatataaac aatttgcgtc ttttggcgtc gggtttgttt cttaatatgt caaaagattg
     4201 gctatcgacg aacaggcgag gtaacgctat gttaacggtg tagctatcag aatacggaag
     4261 gcgcgaagat gaaaaacacc gaactggaac aactgattaa cgaaaaactg aacagcgcgg
     4321 cgattagtga ttacgcgccg aatggtttgc aggtggaagg caaagagacg gtgcaaaaaa
     4381 ttgttaccgg tgtcactgcc agccaggctt tgctcgatga ggcagtgcgt ctgggcgcgg
     4441 atgcggtcat tgtgcatcat ggctacttct ggaaaggcga atctccggtc attcgcggca
     4501 tgaagcgtaa ccgtttaaaa acgttgctgg cgaatgatat caacctgtat ggctggcatt
     4561 tgccgcttga cgcacatcct gagctgggca ataacgcaca gctggcggcg ttactgggga
     4621 ttacggtcat gggcgaaatt gagccgttgg tgccgtgggg cgaactgacc atgcctgtgc
     4681 cgggactgga gctggcttcc tggattgaag cgcgtctggg acgtaagccg ttatggtgtg
     4741 gcgataccgg acctgaggtg gtccagcgcg tcgcctggtg cacgggcggc gggcaaagtt
     4801 ttatcgatag cgccgcgcgt tttggcgtgg atgcttttat tactggcgaa gtttctgaac
     4861 agaccattca ttcagcccgc gagcagggat tgcattttta tgctgcaggt caccatgcca
     4921 ctgaacgtgg tggtattcgc gcattgagcg agtggctgaa tgaaaatacc gatcttgatg
     4981 tgacctttat tgatattcct aatcctgcat aacgaataat cagagggatc gaaagtgcaa
     5041 cgagcgcgtt gttatctgat aggtgaaacg gcggtagtgc tggaactgga accgccggtg
     5101 acgctggcta gccagaaacg gatctggcga ctggcgcagc gtctggtgga tatgccgaat
     5161 gtggttgaag ccattcccgg catgaacaat atcacggtga ttttgcgtaa tcctgagtcg
     5221 ctggcgctgg atgccataga gcgtttgcaa cgctggtggg aggagagcga ggccctggaa
     5281 cctgagtctc gctttattga aattccggtg gtttacggtg gtgcaggcgg accggatttg
     5341 gcggtggtcg cggcgcattg cgggttgagc gaaaaacagg ttgttgaatt gcactcctcc
     5401 gtggaatacg tggtctggtt tttaggtttt caaccgggct tcccgtatct cgggagtttg
     5461 ccggaacaac tacacacgcc acggcgcgct gaaccgcgct tgctcgttcc ggcaggttct
     5521 gtcgggatcg gcgggccgca gactggtgtt tatccgctgg caacgccggg tggctggcag
     5581 ttgattggtc atacctcact cagcctgttt gatccggcgc gtgacgaacc catcttatta
     5641 cgtccgggag acagcgtgcg ctttgtaccg caaaaggagg gagtatgctg aagattattc
     5701 gtgcgggcat gtataccact gtgcaagatg gcggtcgtca cggttttcgc cagtcgggta
     5761 tcagccactg cggcgcactg gatatgcctg cgttacgcat tgctaacctg ctggtgggta
     5821 atgacgccaa tgcccccgcg ctggagatca cgctcggtca gttaacggtt gagttcgaaa
     5881 ctgatgggtg gtttgctctg acgggtgccg gttgcgaagc gcggctggat gataatgccg
     5941 tctggaccgg ctggcgattg ccgatgaaag caggccagcg tttaacgctt aaacgcccgc
     6001 agcacgggat gcgcagttat ctggcggtcg cgggtggtat tgatgttccg ccggtaatgg
     6061 ggtcatgcag caccgatctc aaagtgggga ttggcgggct ggaaggccgt ttactgaagg
     6121 atggtgaccg actcccgatt ggcaaatcga agcgtgattt tatggaagcg cagggcgtta
     6181 aacagctgct gtggggcaac cgcattcgcg ccttgccggg gccggaatat catgagttcg
     6241 atcgcgcctc gcaggatgca ttctggcgtt cgccctggca gcttagcccg caaagtaacc
     6301 gcatgggcta tcgcttacag gggcaaattt taaaacgcac caccgatcgc gaactgttat
     6361 ctcacggttt gttaccgggt gtggtgcagg tgccacataa cgggcagccg attgtgttga
     6421 tgaacgacgc acagaccacc ggtggttacc cgcgtattgc ctgtatcatt gaggctgata
     6481 tgtaccatct ggcgcaaatt ccgctcggtc agccgattca ttttgtccag tgttcactgg
     6541 aagaggcact gaaagcacgg caagatcagc aacgttattt cgaacaatta gcgtggcggc
     6601 tgcacaatga aaattgacct gaacgccgat ctgggcgaag gctgtgccag cgacgcagag
     6661 ctattaacgc tggtttcctc tgccaatatt gcctgtggat ttcatgcagg cgatgcgcaa
     6721 accatgcatg cttgcgtgcg tgaagcaata aaaaatggtg ttgcaattgg cgctcacccg
     6781 agttttcccg acagggaaaa ttttggtcgc agcgccatgc agctgccgcc agaaaccgtt
     6841 tacgcccaga cgctgtatca aattggcgcg ctggcaacga ttgcccgtgc gcaaggcggc
     6901 gtaatgcgtc atgtcaaacc gcacggcatg ttgtacaacc aggcggcgaa agaagcacaa
     6961 ctggcagacg ccatcgccag agcggtatac gcttgcgatc cagcattggt tctcgttggg
     7021 ctggcaggaa gcgagctgat tcgtgcaggc aagcaatatg gcctgacaac gcgcgaggaa
     7081 gtgtttgccg atcgcggtta tcaggctgac ggctcgctgg tgccgcgaaa tcagccaggc
     7141 gcgttgattg aaaacgaaga acaggcgttg gcgcaaacgc tggagatggt gcaacacggc
     7201 agagtcaaaa gtatcaccgg cgaatgggca acggtcacgg cgcaaacggt ctgcctgcat
     7261 ggcgacggcg agcacgcact ggcgttcgcc cgccgactac actctacatt tgccgaaaag
     7321 gggattgttg tcgcagcata accccgatta ataaagaatg aaaaaaggat atcaccatgc
     7381 ctgaaggccc ggagatccgc cgtgcagcgg ataacctgga ggcggcgatc aaaggcaaac
     7441 cactaactga tgtctggttt gccttcccgc agttaaaaac ttatcaatca caacttatcg
     7501 gtcaacacgt tacccatgtg gaaacgcgtg gtaaggcgtt gttaactcat ttttccaacg
     7561 acttaacgct ctacagccat aatcagcttt atggcgtctg gcgcgtggtt gataccggcg
     7621 aagagtcgca gaccacgcga gtattgcggg taaaactgca aacggctgac aaaaccattc
     7681 tgctttatag cgcctcggat attgagatgt tgaccccgga acaactgacc atgcatccgt
     7741 ttttacaacg cgttggtccc gatgtgctgg atccgaatct gacgccggag gtggtgaaag
     7801 aacggttatt gtcgccgcgc tttcgtaacc gtcagtttgc tggattactg ctcgatcagg
     7861 cgtttctggc tgggcttggc aattatttgc gtgtggagat cctctggcag gttgggttga
     7921 ctggaaatca taaagcgaaa gacctcaatg cggcgcaact ggatgcactc gcacacgcgt
     7981 tactggatat tcctcggttt tcttacgcta cgcgggggca ggtggatgag aataagcatc
     8041 atggggcgct gtttcgcttt aaggtttttc atcgtgatgg cgaactgtgc gaacgctgtg
     8101 gcggcatcat tgagaaaacc acgctgtcat ctcgcccgtt ttactggtgt ccgggctgcc
     8161 agcactaggc cgtacgtttc ggcgcatagg ttgaaataaa ccgcgcaatg gcaggccctg
     8221 tcagcaaaat actgaacagg cgcagggttt gcatcgccat aatgagcgcc atatcggcat
     8281 tgctccctgc ggcgatgacc gccacggtat caagcccgcc ggggctggtg gcgaggtagg
     8341 cagtcataaa atcaatgtgc ataaaccggg tcagtcccca cgccataccc gcacaaatag
     8401 ccagcagagc aaaaatcgac agcaggattt gtggtagcgg gcgtagtgcc cgcagtaaga
     8461 tttgtttatc gaaaccaaga ccaatccgcc aaccaattgc catatacgcc atcgccagca
     8521 gccattctgg tagttcgatg gtgatgagct gaccagactg gagcactgcg cacgccagca
     8581 tcgggatgag catcgtgccg gaaggaagac gcagcaggca tcccactgta ccggcaacga
     8641 ccgcgagcaa aatagtaagc aggagattaa tgctcactgg cgggaaccag acgatatgct
     8701 ggttaaccgc ttcagcgtta tcgcccagca tcattcgcgt gaccagaact gcggcccccg
     8761 cgacaaacag cactcgcaga tactgcataa acgccaccag gcggatatct gcgccgtaat
     8821 cctgcgccat cgcgaccatt gccgccgccc cgccaggcga ggagccccag gcaccagtat
     8881 ttccgggcag tgagctatag cgcaccagta gccatcccac gatagcgctg gaaagcaacg
     8941 tcaccagtaa aatagctaac acaatcggcc agttaaccgc cagcgtggtc aggatcgaac
     9001 cggtcaagtt ttgcgcaatc atgcagccaa gaatggcctg cgcggcgaga aaagcggagc
     9061 gggggagttg cagggtaacc ccgcgcatac tgaagatgat cccggcgatc atcggcccaa
     9121 gtaataatgc cgccgggaga tgtaccgcga ggaaaccaat agaaaggaga agtgatagca
     9181 cacataacat tccccactgc aaaactggca tcctttgctc cctcattatt ttctctttgg
     9241 tgtatcgata cagcataaag aatgccgggc atggaaaaaa taacggtaat caataaaagc
     9301 cggaaaattg gcaattaacc gaagaagccc tctccggctg gaaagggcaa taggctaaat
     9361 cagttgtagt taattttgaa aagtaccact gtatcaaagg gaccaacgtc gatgggcgta
     9421 cccggcgtgg atgacaattc tgccgtgtag gttttttcat acactaacag gtcgctggta
     9481 aaatcggcat actcactgta tttgttgaaa gtatagggtg aggcattttc atccaacaat
     9541 cgaagcttca ggccattgcc aatgagcagt gatttatctt cgtcgataag ggttttatcg
     9601 gtataaaacg aagagctgac tttaaaaccg tcggtacact ggtcgttttg tgatttggtt
     9661 gtagttattt taaattgctg cgacatcgta tggcgacgaa tatccagcac gttaaatcgg
     9721 ccaaaatcaa cgacctgggt ttcaggtgag atagcaaagt tgacgctgca atccagcacg
     9781 cggatatttt ccagaccggt aatgtgatat ttcagattct tcgcggtagg gtcttcgttc
     9841 acgctgcctg cgccatcaaa ctgtacgacg atgtaatcgc tgagtttgct ctgataatca
     9901 tggggcggca tggcgcgaac tttaacgtaa agacgcatac gcgccacaaa cgtccgcgat
     9961 ttgtgcataa cggttggatc gccggagcaa agtttatttt gccagcccat cgcaatgatc
    10021 tgctccgggg tgtaaatatc aatattctta ctgtcgatgc actgattcgt gtcgatgcgg
    10081 cttttcccaa ggcttgcgtc atagtccacg ccttcatagg ttacgcccaa ttgataatag
    10141 ggatcctgaa taccggggta agggtttacc caggcgtaga cgtgctcgct ttcaaaatta
    10201 ccgtttgtgt tgttgtcaca gtagaccgga atttttatat cgtcagattc ccagatttta
    10261 tcgccgggtt tggaattggc cggaacggcg aagggcataa tagcttccga tttttctaca
    10321 gcgccgtttg cggtaccaaa atagcagttc agcgccagcg ccgcttttag cggcaaaaaa
    10381 atcagacaga tggcgagcaa tacgcctttg gcgaatctca ttttttattc tccaccggct
    10441 gcaggcggca ttgccgctgg caggagagcg tgttcatacg caatgcgccg tagtcgtcca
    10501 tgtagccaag ataaaactgc ttgccgctat agctgcccgt gctggtggtg accgaactga
    10561 acggtgcgac catcgtgctt ttaaagccag gcagcacgcc tttctcatcc cgcccaagat
    10621 aggcaatagt cagatagtat ccggtcgggt ttttcagggt aagctggtta ttctgctggc
    10681 taacctgtag ctgttgctca acatgatcgc ccattttttt gcgtaatgcg gcgggtcgcc
    10741 agaatagctt aatgcgactt tggacggcga cttgcaggat cgcatggccc tcgctgcctt
    10801 ccgggacagg cggaatttca cgcaggttat agtaaaagag agattcgcga tcggtgggta
    10861 atttcgcggt ggcggcttgt ttcatgatcc gaacctgcga agtcgctttt ggctccagtc
    10921 gctggatagg cggcagggcg acgagaaatt catcgctttt ctctcccttt tcattttcta
    10981 tccatgaata ggcgaggtag gggagtttat cgctgcggtt ttccacgcgc aggctggttg
    11041 ctttatcttt gctattaaag atgatgcgcg tgcggtcagg ctgcactgcg ccgtggctgc
    11101 acatactggc aaccagaaga atcaaaggga atcctttaac aaatgtcatc gtcttttcct
    11161 taattaatga cagggtaaaa tcaggcgctt agtcacatct tccagatgtt ccggcagatg
    11221 aatggcacac gttttctggt cgccccagtg cacggtaaat tgttgatttt catcgacccc
    11281 actcagccat gcgtgaccgt tttcgccaac catcccgacg ctgacaccgc cttcagcctg
    11341 gcgaacatcg gcaccgagcg gcgggaaatg cccgttagca tcgctgataa tgacgttgag
    11401 atctttcccg gcgcgggacg ccagcgattt aaagccgatg gcgccttcgg tccaggtttc
    11461 tttcaccacg ttttcggaaa cggtaacgcc gtcaggcaag tcgttcatat tgaccgccac
    11521 cgtcgtaggc tggtagctgg aaacaaacgg taccacggca ataccaaagc ggttggtgta
    11581 gtcaatattg ccctgaatcg gaatatcgcc cacgccgtcg gtgctgacca tcaggcgtgg
    11641 ttcattggtg gaactgcggc gatggaatgc cgcgccatgt tgagtcgcgg taaacgagcc
    11701 gctccaactg gcactggcgg aagtgtaatc attggcggca taggttccgg tcagatccca
    11761 ttcccccgcc gaactgaggt gttggtaatt gccgctgacc tgcgcaccgt tatccgggcg
    11821 atcggattgt attccagccg acattcccca actgtttcgt tcatccaggg tatcgttcca
    11881 cgacatgcgg tgggtggtag tattactgtt gttctgcatg tcataaccca gtcgaccact
    11941 ttccccaatc ggtaaggaga tcgaaaagta aatctggttg tcgcgatctt tatcttcata
    12001 atgggtggtg ttgaacgagg tcgagacaga aatatctttc cagtcgccaa tgtcgacgtt
    12061 aaaaccggcg gtaatgttgg ccgtggtgga ggtatccgca ttccaccagc tctgatgcag
    12121 gatgttggcg tagagattaa gattcagcgg agtaataggc tgaccgaagg agaggctgat
    12181 ggtttgcttt tcatcctgcg cgtcggcgtc attgtatttg tgatcaatgt agttagcgta
    12241 gctgtgaaaa tggcgatcgg agaagcgata ggctgccaat gagatggtgc tgttggtcgc
    12301 atcaacctgt ttgctgtagt taaagcggta gctgtaaccc tgctcatcct gttggatatc
    12361 aaaatggctg tcggcccagg tcacgtcaaa cgacagcgcc cccagccaga gcatattttg
    12421 cccaataccc agcgcgccgg aacgatagtc atcgcctgcg agaagcatgc cgccatatag
    12481 tgaagtgttg gagagcatcc cccaggagac ttcatggcta atgaaggttt catcctcggt
    12541 gtggtgcgac atagaggaac gcgggcgacc ggcagccagt ttatagcgca cctggccctg
    12601 acgcgtcagg aacggggtag aggccgctga aacctggaag ttattcaccc gtccatcttc
    12661 ttcgctcact ttgacatcca gtgtaccttg cactgactga ttcaggtcat caataataaa
    12721 tgggcctggc ggaacttttt tctggtaaat cacgcgacca gaatggctta tcgtgaccgt
    12781 ggcgttagtc tgtgcgatgc cgctgatttg cggcgcgtag ccgcgtaact cccaggggag
    12841 cattcgatcg tcgctggcta acgccgcacc ggtataggag aaaccatcaa aaatgttaga
    12901 gctaaaatcg gtttcgccca gggttaatcg ggaacctatt tgcggtaacg ggcggaacag
    12961 gtaggtccgt gaaatcgccc cggattgttc acggttgtcg ccgctatcgg actggctaag
    13021 ctgataatcg ctgcgcagac gccatgcccc ggcgttgagg cccgtggtgc cataggcgtt
    13081 caggttgttg ctgctgctgc cgctttgtgg gcgataagtg ctggcaaaca ggttgtaatc
    13141 catcaggaag ccaggaatac cgttattcca cgttgagggt ggcgtccagt tttctgaatg
    13201 ccacgccagc catgcctgcg ggattgtaat attgagctgt tgatttgcct gatcgaaagt
    13261 aaagatgatt tcagggaaga cactaaaatc gacacattct tttattgccg gtaatttttt
    13321 acgaaagtca gatttaagtc cgaagagttc gatcagcgat tcattgatac aaggaataat
    13381 tttatcgcca gatttttgcc agcgaatttg ttgcccatta ctgattttat tattattaac
    13441 cgtaacacta acaaagtaat cgccgggagc tacgacgcct ttttgattga gcaacgaaat
    13501 atcaacgcta tcccgaatcg acttatcgag aacgttaagg ttaaattcag ctgccagcgc
    13561 aggaggggtt accattgcca gacaggaaag aacagagagt cgataaatat tcacggcatc
    13621 catacctgaa caatattcgc aaaaggcggc taatcagccg ccttaaaaag gattattggt
    13681 aatccagaac gtaagttgcg ttagcagtaa cggcccccgc ggtaactgaa actgcgttat
    13741 ccatctgttc catccaggca aagaagttta acgtttgttc tgttgaactt gccaataagt
    13801 caattgttgg cgcttctgtt cccaatacaa caaatgattc atctttgttc atgagacgta
    13861 cgccaacgcc ggtagcctcg ccagcactga tgttactaag gagcggcgtt gcacctgtcg
    13921 ttttagccgt gctgtcgaag gtcacgccta cctttgagac cacgccaccc ttgccatcat
    13981 cccagcctgc aatatcacag ttgattaagc gaatattgac aggttttttc tcgctatgat
    14041 gttctcgatt tatgtgggat gtggttactt cacccaggtc aatgttcata tcaaggtcat
    14101 cagggtgaat ttcacaaggt gcttcaataa cttcaccttt aaagtggatt tcaccagatc
    14161 cttcatctgc agcaaaaaca ggtgaagcga atagtagaga tatggccagg gcggccagtg
    14221 ttttttgtcc tttaaacata catgaatcct tcatgggcta tgataaagta tactaattat
    14281 tccgacagaa tagtggtttt ttgttcatgt aataatatta atgttgctac attatgtttg
    14341 agagtttctc aaagctctcg aatcataaat aaagtgaatt aatcctatta cgagtttatt
    14401 taattgtggg tgctacccaa tgtattctac atcgattggc gggataacgg tggtggtgaa
    14461 tgaaaaatta ggattttatt tagttatcgg atatgtattc ttgtaacgtg taattatgta
    14521 tttgtgtccg attacgtttt tttgtcgata gacataacta acgaacaaaa aaaagcccgc
    14581 cagatattca ggcgggctaa acgatatatg gttcaaatca gataattaat gtttaacatc
    14641 tgatttaaag tctcgttttt catagccggt gtacaactgg cgcggacggg cgatcttcat
    14701 gccgtcactg tgcatttcgc tccagtgagc gatccagcca acggtacggg ccatagcgaa
    14761 gatgacggtg aacatggaag acggaatacc catcgctttc aggatgatac cagagtagaa
    14821 atcgacgttc gggtacagtt tcttctcgat aaagtacggg tcgttcagcg cgatgttttc
    14881 cagctccata gccacttcca gcaggtcatc tttggtgccc agctctttca gaacttcatg
    14941 gcaggtttca cgcattacgg tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa
    15001 gcccatcagg cggaaagaat catttttatc tttcgcacga cgaacaaatt ccggaatgtg
    15061 tttaacggag ctaatttctt ccagcatttt cagcgccgct tcgttagcac caccgtgcgc
    15121 aggcccccac agtgaagcaa tacctgctgc gatacaggca aacgggttcg cacccgaaga
    15181 gccagcggta cgcacggtgg aggtagaggc gttctgttca tggtcagcgt gcaggatcag
    15241 aatacggtcc atagcacgtt ccagaatcgg attaacttca tacggttcgc acggcgtgga
    15301 gaacatcata ttcaggaagt taccggcata ggagagatcg ttgcgcggat aaacaaatgg
    15361 ctgaccaatg gaatacttgt aacacatcgc tgccatggtc ggcattttcg acagcaggcg
    15421 gaacgcggca atttcacgat gacgaggatt gttaacatcc agcgagtcgt gatagaaagc
    15481 cgccagcgcg ccggtaatac cacacatgac tgccattgga tgtgagtcgc gacggaaagc
    15541 gtggaacaga cgggtaatct gctcgtggat catggtatga cgggtcaccg tagttttaaa
    15601 ttcgtcatac tgttcctgag tcggtttttc accattcagc aggatgtaac aaacttccag
    15661 gtagttagaa tcggtcgcca gctgatcgat cgggaaaccg cggtgcagca aaataccttc
    15721 atcaccatca ataaaagtaa ttttagattc gcaggatgcg gttgaagtga agccagggtc
    15781 aaaggtgaac acaccttttg aaccgagagt acggatatca ataacatctt gacccagcgt
    15841 gcctttcagc acatccagtt caacagctgt gtccccgttg agggtgagtt ttgcttttgt
    15901 atcagccatt taaggtctcc ttagcgcctt attgcgtaag actgccggaa cttaaatttg
    15961 ccttcgcaca tcaacctggc tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag
    16021 gggaaaacct gggtacagag ctctgggcgc ttgcaggtaa aggatccatt gatgacgaat
    16081 aaatggcgaa tcaagtactt agcaatccga attattaaac ttgtctacca ctaataactg
    16141 tcccgaatga attggtcaat acttccacac tgttacataa gttaatctta ggtgaaatac
    16201 cgacttcata acttttacgc attatatgct tttcctggta atgtttgtaa caactttgtt
    16261 gaatgattgt caaattagat gattaaaaat taaataaatg ttgttatcgt gacctggatc
    16321 actgttcagg ataaaacccg acaaactata tgtaggttaa ttgtaatgat tttgtgaacg
    16381 gcctatactg ccgccaggtc tccggaacac cctgcaatcc cgagccaccc agcgttgtaa
    16441 cgtgtcgttt tcgcatctgg aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc
    16501 tgtctgaccc gcaagcagac cggaggaagg aaatcccgac gtctccaggt aacagaaagt
    16561 taacctctgt gcccgtagtc cccagggaat aataagaaca gcatgtgggc gttattcatg
    16621 ataagaaatg tgaaaaaaca aagacctgtt aatctggacc tacagaccat ccggttcccc
    16681 atcacggcga tagcgtccat tctccatcgc gtttccggtg tgatcacctt tgttgcagtg
    16741 ggtatcctgc tgtggcttct gggtaccagc ctctcttccc ctgaaggttt cgagcaagct
    16801 tccgcgatta tgggcagctt cttcgtcaaa tttatcatgt ggggcatcct taccgctctg
    16861 gcgtatcacg tcgtcgtagg tattcgccac atgatgatgg attttggcta tctggaagaa
    16921 acattcgaag cgggtaaacg ctccgccaaa atctcctttg ttattactgt cgtgctttca
    16981 cttctcgcag gagtcctcgt atggtaagca acgcctccgc attaggacgc aatggcgtac
    17041 atgatttcat cctcgttcgc gctaccgcta tcgtcctgac gctctacatc atttatatgg
    17101 tcggtttttt cgccaccagt ggcgagctga catatgaagt ctggataggt ttcttcgcct
    17161 ctgcgttcac caaagtgttc accctgctgg cgctgttttc tatcttgatc catgcctgga
    17221 tcggcatgtg gcaggtgttg accgactacg ttaaaccgct ggctttgcgc ctgatgctgc
    17281 aactggtgat tgtcgttgca ctggtggttt acgtgattta tggattcgtt gtggtgtggg
    17341 gtgtgtgatg aaattgccag tcagagaatt tgatgcagtt gtgattggtg ccggcggcgc
    17401 aggtatgcgc gcggcgctgc aaatttccca gagcggccag acctgtgcgc tgctctctaa
    17461 agtcttcccg acccgttccc ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg
    17521 taatacccat gaagataact gggaatggca tatgtacgac accgtgaaag ggtcggacta
    17581 tatcggtgac caggacgcga ttgaatatat gtgtaaaacc gggccggaag cgattctgga
    17641 actcgaacac atgggcctgc cgttctcgcg tctcgatgat ggtcgtatct atcaacgtcc
    17701 gtttggcggt cagtcgaaaa acttcggcgg cgagcaggcg gcacgtactg cggcagcggc
    17761 tgaccgtacc ggtcacgcac tgttgcacac gctttatcag cagaacctga aaaaccacac
    17821 caccattttc tccgagtggt atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt
    17881 gggttgtacc gcactgtgca tcgaaaccgg tgaagtggtt tatttcaaag cccgcgctac
    17941 cgtgctggcg actggcggag cagggcgtat ttatcagtcc accactaacg cccacattaa
    18001 cactggcgac ggcgtcggca tggctatccg tgcaggcgta ccggtgcagg atatggaaat
    18061 gtggcaattc cacccgaccg gcattgccgg tgcgggcgta ctggtcaccg aaggttgccg
    18121 tggtgaaggc ggttatctgc tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc
    18181 gaacgccaaa gacctggcgg gccgtgatgt ggtggcgcgt tccatcatga tcgaaatccg
    18241 tgaaggtcgc ggctgtgatg gtccgtgggg gccacacgcg aaactgaaac tcgatcacct
    18301 gggtaaagaa gttctcgaat cccgtctgcc gggtatcctg gagctttccc gtaccttcgc
    18361 tcacgttgat ccggtgaaag agccgattcc ggttatccca acctgtcact acatgatggg
    18421 cggtattccg accaaagtta ccggtcaggc gctgactgtg aatgagaaag gcgaagatgt
    18481 ggttgttccg gggctgttcg cggtaggtga aattgcctgc gtatcggtac acggtgctaa
    18541 ccgtctgggc ggcaactcgc tgctggacct ggtggtcttt ggtcgcgcag cgggtctgca
    18601 tctgcaagag tctatcgccg agcagggcgc attgcgcgat gccagcgagt ctgatgttga
    18661 agcatctttg gatcgtctga accgctggaa caataatcgt aacggtgaag atccggtggc
    18721 gattcgtaaa gcgctgcaag aatgtatgca gcataacttc tcggtcttcc gtgaaggtga
    18781 tgcgatggcg aaagggcttg agcagttgaa agtgatccgc gagcgtctga aaaatgcccg
    18841 tctggatgac acttccagcg agttcaacac ccagcgcgtt gagtgcctgg aactggataa
    18901 cctgatggaa acggcgtatg caacggctgt ttctgccaac ttccgtaccg aaagccgtgg
    18961 cgcgcatagc cgcttcgact tcccggatcg tgatgatgaa aactggctgt gccactccct
    19021 gtatctgcca gagtcggaat ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg
    19081 cccggcattc ccgccgaaga ttcgtactta ctaatgcgga gacaggaaaa tgagactcga
    19141 gttttcaatt tatcgctata acccggatgt tgatgatgct ccgcgtatgc aggattacac
    19201 cctggaagcg gatgaaggtc gcgacatgat gctgctggat gcgcttatcc agctgaaaga
    19261 gaaagatccc agcctgtcgt tccgtcgctc ctgtcgtgaa ggtgtgtgcg gttccgacgg
    19321 tctgaacatg aacggcaaga atggtctggc ctgtattacc ccgatttcgg cactcaacca
    19381 gccgggcaag aagattgtga ttcgcccgct gccaggttta ccggtgatcc gcgatttggt
    19441 ggtagacatg ggacaattct atgcgcaata tgagaaaatt aagccttacc tgttgaataa
    19501 tgaacaaaat ccgccagctc gcgagcattt acagatgcca gagcagcgcg aaaaactcga
    19561 cgggctgtat gaatgtattc tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg
    19621 gaatcccgat aagtttatcg gcccggcagg cttgttagcg gcatatcgtt tcctgatcga
    19681 tagccgtgat accgagactg acagccgcct cgacggtttg agcgatgcat tcagtgtatt
    19741 ccgctgtcat agcatcatga actgcgtcag tgtatgtccg aaggggctga acccgacgcg
    19801 cgccatcggc catatcaagt cgatgttgtt gcaacgtaat gcgtaaaccg taggcctgat
    19861 aagacgcgca agcgtcgcat caggcaacca gtgccggatg cggcgtgaac gccttatccg
    19921 gcctacaagt cattacccgt aggcctgata agcgcagcgc atcaggcgta acaaagaaat
    19981 gcaggaaatc tttaaaaact gcccctgaca ctaagacagt ttttaaaggt tccttcgcga
    20041 gccactacgt agacaagagc tcgcaagtga accccggcac gcacatcact gtgcgtggta
    20101 gtatccacgg cgaagtaagc ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc
    20161 tttgaaagcc tggttggact cttcttacct ctctggcgca aaccagagct ggatagaaca
    20221 gctctatgaa gacttcttaa ccgatcctga ctcggttgac gctaactggc gttcgacgtt
    20281 ccagcagtta cctggtacgg gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata
    20341 tttccgccgc ctggcgaaag acgcttcacg ttactcttca acgatctccg accctgacac
    20401 caatgtgaag caggtaaaag tcctgcagct cattaacgca taccgcttcc gtggtcacca
    20461 gcatgcgaat ctcgatccgc tgggactgtg gcagcaagat aaagtggccg atctggatcc
    20521 gtctttccac gatctgaccg aagcagactt ccaggagacc ttcaacgtcg gttcatttgc
    20581 cagcggcaaa gaaaccatga aactcggcga gctgctggaa gccctcaagc aaacctactg
    20641 cggcccgatt ggtgccgagt atatgcacat caccagcacc gaagaaaaac gctggatcca
    20701 acagcgtatc gagtctggtc gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag
    20761 cgaactgacc gccgctgaag gccttgaacg ttacctcggt gcaaaattcc ctggtgcaaa
    20821 acgcttctcg ctggaaggcg gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca
    20881 cgctggcaac agcggcaccc gcgaagtggt tctcgggatg gcgcaccgtg gtcgtctgaa
    20941 cgtgctggtg aacgtgctgg gtaaaaaacc gcaagacttg ttcgacgagt ttgccggtaa
    21001 acataaagaa cacctcggca cgggtgacgt gaaataccac atgggcttct cgtctgactt
    21061 ccagaccgat ggcggcctgg tacacctggc gctggcgttt aacccgtctc accttgagat
    21121 tgtcagcccg gtcgttatcg gttctgttcg tgcccgtctg gacagacttg atgagccgag
    21181 cagcaacaaa gtgctgccaa tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt
    21241 ggttcaggaa actctgaaca tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg
    21301 tatcgttatc aacaaccagg ttggtttcac cacctctaat ccgctggatg ctcgttctac
    21361 accgtactgt actgatatcg gtaagatggt tcaggcaccg attttccacg ttaacgcgga
    21421 tgatccggaa gccgttgcct ttgtgacccg tctggcgctc gatttccgta acacctttaa
    21481 acgtgatgtc ttcatcgacc tggtgtgcta ccgccgtcac ggccacaacg aagccgacga
    21541 gccgagcgca acccagccgc tgatgtatca gaaaatcaaa aaacatccga cgccgcgcaa
    21601 aatctatgct gacaagttgg agcaggaaaa agtggcgacg ctggaagatg ccaccgagat
    21661 ggttaacctg taccgcgatg cgctggatgc tggcgattgc gtagtggcag agtggcgtcc
    21721 gatgaacatg cactctttca cctggtcgcc gtatctcaac cacgaatggg acgaagagta
    21781 cccgaacaaa gttgagatga agcgcctgca ggaactggct aaacgcatca gcacggtgcc
    21841 ggaagcggtt gaaatgcagt ctcgcgttgc caaaatttat ggcgatcgcc aggcgatggc
    21901 tgccggtgag aaactgttcg actggggcgg cgcggaaaac ctcgcttacg ccacgctggt
    21961 tgacgaaggc attccggttc gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca
    22021 ccgccacgcg gtgatccaca accagtctaa cggttccact tacacgccgc tgcaacacat
    22081 ccataacggc cagggcgcgt tccgtgtctg ggactccgta ctgtctgaag aagcagtgct
    22141 ggcgtttgaa tacggttatg ccaccgcaga accacgcact ctgactatct gggaagcgca
    22201 gttcggtgac ttcgccaacg gtgcgcaggt ggttatcgac cagttcatct cctctggcga
    22261 acagaaatgg ggccggatgt gtggtctggt gatgttgctg ccgcacggtt atgaagggca
    22321 ggggccggag cactcctccg cgcgtctgga acgttatctg caactttgcg ctgagcaaaa
    22381 catgcaggtg tgcgtaccgt ctaccccggc acaggtttac cacatgctgc gtcgccaggc
    22441 gctgcgcggg atgcgtcgtc cgctggtcgt gatgtcgccg aaatccctgc tgcgtcatcc
    22501 gctggcggtt tccagcctcg aagaactggc gaacggcacc ttcctgccag ctatcggtga
    22561 aatcgacgag cttgatccga agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta
    22621 ttacgacctg ctggaacagc gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat
    22681 cgagcaactc tacccgttcc cgcataaagc gatgcaggaa gtgttgcagc agtttgctca
    22741 cgtcaaggat tttgtctggt gccaggaaga gccgctcaac cagggcgcat ggtactgcag
    22801 ccagcatcat ttccgtgaag tgattccgtt tggggcttct ctgcgttatg caggccgccc
    22861 ggcctccgcc tctccggcgg tagggtatat gtccgttcac cagaaacagc aacaagatct
    22921 ggttaatgac gcgctgaacg tcgaataaat aaaggataca caatgagtag cgtagatatt
    22981 ctggtccctg acctgcctga atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa
    23041 cccggcgacg cagtcgtacg tgatgaagtg ctggtagaaa tcgaaactga caaagtggta
    23101 ctggaagtac cggcatcagc agacggcatt ctggatgcgg ttctggaaga tgaaggtaca
    23161 acggtaacgt ctcgtcagat ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa
    23221 accagcgcca aatctgaaga gaaagcgtcc actccggcgc aacgccagca ggcgtctctg
    23281 gaagagcaaa acaacgatgc gttaagccca gcgatccgtc gcctgctggc tgaacacaac
    23341 ctcgacgcca gcgccattaa aggcaccggt gtgggtggtc gtctgacccg tgaagatgtg
    23401 gaaaaacatc tggcgaaagc cccggcgaaa gagtctgctc cggcagcggc tgctccggcg
    23461 gtgcaaccgg ctctggctgc acgtagcgaa aaacgtgtcc cgatgactcg cctgcgtaag
    23521 cgtgtggcag agcgtctgct ggaagcgaaa aactccaccg ccatgctgac cacgttcaac
    23581 gaagtcaaca tgaagccgat tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa
    23641 cgccacggca tccgtctggg ctttatgtcc ttctacgtga aagcggtggt tgaagccctg
    23701 aaacgttacc cggaagtgaa tgcttctatc gacggcgatg acgtggttta ccacaactat
    23761 ttcgacgtca gcatggcggt ttctacgccg cgcggcctgg tgacgccggt actgcgtgat
    23821 gtcgataccc tcggcatggc agacatcgag aagaaaatca aagagctggc agttaaaggc
    23881 cgtgacggca agctgacggt tgaagatctg accggtggta acttcaccat caccaacggt
    23941 ggtgtgttcg gttccctgat gtctacgccg atcatcaacc cgccgcagag cgcaattctg
    24001 ggtatgcacg ctatcaaaga tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg
    24061 atgatgtacc tggcgctgtc ctacgatcac cgtctgatcg atggtcgcga atccgtgggc
    24121 ttcctggtaa cgatcaaaga gttgctggaa gatccgacac gtctgctgct ggacgtttag
    24181 tagtttaagc ttcacctgca ctgtagaccg ggtaagacat tatcgccttc tccggcaatt
    24241 gaagcctgat gcgacgctta acgcgtctta tcaggcctac gggaagcacc gatgtaggtc
    24301 ggataaggcg ctcgcgtcgc atccgacaag cgatgcctga tgcgacgctt acacgtctta
    24361 tcaggcctac gggtgaccga caatacccgg gagcgatacg aaatattcgg tctacggttt
    24421 aaaagataac gattactgaa ggatggacag aacacatgaa cttacatgaa tatcaggcaa
    24481 aacaactgtt tgcccgctat ggcttaccag caccggtggg ttatgcctgt actactccgc
    24541 gcgaagcaga agaagccgct tcaaaaatcg gtgccggtcc gtgggtagtg aaatgtcagg
    24601 ttcacgctgg tggccgcggt aaagcgggcg gtgtgaaagt tgtaaacagc aaagaagaca
    24661 tccgtgcttt tgcagaaaac tggctcggca agcgtctggt aacgtatcaa acagatgcta
    24721 atggccaacc ggttaaccag attctggttg aagcagcgac cgatatcgct aaagagctgt
    24781 atctcggtgc cgttgtagac cgtagttccc gtcgcgtggt ctttatggcc tccaccgaag
    24841 gcggcgtgga aatcgaaaaa gtggcggaag aaactccgca tctgatccac aaagttgcgc
    24901 ttgatccact gactggcccg atgccgtatc agggacgcga gctggcgttc aaactgggtc
    24961 tggaaggtaa actggttcag cagttcacca aaatcttcat gggcctggcg accattttcc
    25021 tggaacgcga tctggcgctg atcgaaatca acccgctggt catcaccaaa cagggtgatc
    25081 tgatttgcct cgacggcaaa ctgggcgctg atggcaacgc actgttccgc cagcctgatc
    25141 tgcgcgaaat gcgtgaccag tcgcaggaag atccacgtga agcacaggct gcacagtggg
    25201 aactgaacta cgttgcgctg gacggtaaca tcggttgtat ggttaacggc gcaggtctgg
    25261 cgatgggtac gatggacatc gttaaactgc acggcggcga accggctaac ttccttgacg
    25321 ttggcggcgg cgcaaccaaa gaacgtgtaa ccgaagcgtt caaaatcatt ctctctgacg
    25381 acaaagtgaa agccgttctg gttaacatct tcggcggtat cgttcgttgc gacctgatcg
    25441 ctgacggtat catcggcgcg gtagcggaag tgggtgttaa cgtaccggtc gtggtacgtc
    25501 tggaaggtaa caacgccgaa ctcggcgcga agaaactggc tgacagcggc ctgaatatta
    25561 ttgcagcaaa aggtctgacg gatgcagctc agcaagttgt tgccgcagtg gaggggaaat
    25621 aatgtccatt ttaattgata aaaacaccaa ggttatctgc cagggcttta ccggtagcca
    25681 ggggactttc cactctgaac aggccattgc atacggcacc aaaatggttg gcggtgtaac
    25741 cccaggtaaa ggcggtacta cccacctcgg cctgccggtg ttcaacaccg tgcgtgaagc
    25801 tgttgctgct actggcgcta ccgcttctgt tatctacgta ccagcaccgt tctgcaaaga
    25861 ctccattctg gaagccatcg acgcaggcat caaactgatt atcaccatca ctgaaggcat
    25921 cccgacgctg gatatgctga ctgtgaaagt gaaactggac gaagcaggcg ttcgtatgat
    25981 cggcccgaac tgcccaggcg ttatcacccc gggtgaatgc aaaattggta tccaaccggg
    26041 tcacattcac aaaccgggta aagtgggtat cgtttcccgt tccggtacac tgacctatga
    26101 agcggttaaa cagaccacgg attatggttt cggtcagtcg acctgtgtcg gtatcggcgg
    26161 tgacccgatc cctggctcta actttatcga cattctcgaa atgttcgaaa aagatccgca
    26221 gaccgaagcg atcgtgatga tcggtgagat cggcggtagc gctgaagaag aagcagctgc
    26281 gtacatcaaa gagcacgtta ccaagccagt tgtgggttac atcgctggtg tgactgcgcc
    26341 gaaaggcaaa cgtatgggcc acgcgggtgc catcattgcc ggtgggaaag ggactgcgga
    26401 tgagaaattc gctgctctgg aagccgcagg cgtgaaaacc gttcgcagcc tggcggatat
    26461 cggtgaagca ctgaaaactg ttctgaaata aatatctgta ataagaaata gccctcgccg
    26521 cttccctcta caggaatgcg aagggctgtc ggtttcgaca tggttggcca tcgtatgatg
    26581 gcctttttta tgccttaaac ggttaaccac ggtcgaaatc tgactgcagg cttgactgca
    26641 tgaccacagc cactgtggat tgccgcgacc gaccgcaagc catgaataag aacgacggcg
    26701 aatttcgaat cgttacctga tgcactggct tcccagcgtt tgaccgtagc gttaagtaaa
    26761 tcaacaaaag cgatgttcac cgcttttcgt caagtcgtgc catttgtcag ccaggatcgg
    26821 gtctttaccc cggatattgc agcatcgcaa a
//
LOCUS       DAZUCE010000046        26513 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZUCE010000046 DAZUCE010000000
VERSION     DAZUCE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.046"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            27..695
                     /gene="pspA"
                     /locus_tag="ACHY03_001628"
     CDS             27..695
                     /gene="pspA"
                     /locus_tag="ACHY03_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFS3904872.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            749..973
                     /gene="pspB"
                     /locus_tag="ACHY03_001629"
     CDS             749..973
                     /gene="pspB"
                     /locus_tag="ACHY03_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFS3904873.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            973..1332
                     /gene="pspC"
                     /locus_tag="ACHY03_001630"
     CDS             973..1332
                     /gene="pspC"
                     /locus_tag="ACHY03_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFS3904874.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            1341..1562
                     /gene="pspD"
                     /locus_tag="ACHY03_001631"
     CDS             1341..1562
                     /gene="pspD"
                     /locus_tag="ACHY03_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFS3904875.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            1637..1951
                     /gene="pspE"
                     /locus_tag="ACHY03_001632"
     CDS             1637..1951
                     /gene="pspE"
                     /locus_tag="ACHY03_001632"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFS3904876.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATVVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            2162..3841
                     /gene="ycjM"
                     /locus_tag="ACHY03_001633"
     CDS             2162..3841
                     /gene="ycjM"
                     /locus_tag="ACHY03_001633"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFS3904877.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     RTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPDVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSGKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     YREEITRELNDEATLRHAVYHELSRLIILRRSHNEFHPDNNFTIDTVNSSVMRIQRSN
                     AEGNCLTGLFNVSKNIQHVNITGLHGRDLISEVDILGNKITLRPWQVMWIK"
     gene            3855..5146
                     /gene="ycjN"
                     /locus_tag="ACHY03_001634"
                     /pseudo
     CDS             3855..5146
                     /gene="ycjN"
                     /locus_tag="ACHY03_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            5167..6048
                     /gene="ycjO"
                     /locus_tag="ACHY03_001635"
     CDS             5167..6048
                     /gene="ycjO"
                     /locus_tag="ACHY03_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS3904878.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            6035..6877
                     /gene="ycjP"
                     /locus_tag="ACHY03_001636"
     CDS             6035..6877
                     /gene="ycjP"
                     /locus_tag="ACHY03_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFS3904879.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            6908..7960
                     /gene="ycjQ"
                     /locus_tag="ACHY03_001637"
     CDS             6908..7960
                     /gene="ycjQ"
                     /locus_tag="ACHY03_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFS3904880.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVNDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            7978..8766
                     /gene="ycjR"
                     /locus_tag="ACHY03_001638"
     CDS             7978..8766
                     /gene="ycjR"
                     /locus_tag="ACHY03_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFS3904881.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENNLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            8776..9831
                     /gene="ycjS"
                     /locus_tag="ACHY03_001639"
     CDS             8776..9831
                     /gene="ycjS"
                     /locus_tag="ACHY03_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS3904882.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            9828..12095
                     /gene="ycjT"
                     /locus_tag="ACHY03_001640"
     CDS             9828..12095
                     /gene="ycjT"
                     /locus_tag="ACHY03_001640"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFS3904883.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIVINGEVFSLSREAWQRELDFASGELR
                     RSVVWCTSNGARYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQATGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAYTSYMACYNVQQALNIARQFGCSDDA
                     FIHRAEIFLKELWMPETQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAVSCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            12092..12751
                     /gene="pgmB"
                     /locus_tag="ACHY03_001641"
     CDS             12092..12751
                     /gene="pgmB"
                     /locus_tag="ACHY03_001641"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFS3904884.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAKIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            12765..13847
                     /locus_tag="ACHY03_001642"
     CDS             12765..13847
                     /locus_tag="ACHY03_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3904885.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKMVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            13892..14797
                     /gene="ompG"
                     /locus_tag="ACHY03_001643"
     CDS             13892..14797
                     /gene="ompG"
                     /locus_tag="ACHY03_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFS3904886.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(14908..15906)
                     /gene="ycjW"
                     /locus_tag="ACHY03_001644"
     CDS             complement(14908..15906)
                     /gene="ycjW"
                     /locus_tag="ACHY03_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS3904887.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            16062..17459
                     /gene="ycjX"
                     /locus_tag="ACHY03_001645"
     CDS             16062..17459
                     /gene="ycjX"
                     /locus_tag="ACHY03_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFS3904888.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            17456..18517
                     /gene="ycjF"
                     /locus_tag="ACHY03_001646"
     CDS             17456..18517
                     /gene="ycjF"
                     /locus_tag="ACHY03_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFS3904889.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            18665..20206
                     /gene="tyrR"
                     /locus_tag="ACHY03_001647"
     CDS             18665..20206
                     /gene="tyrR"
                     /locus_tag="ACHY03_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFS3904890.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(20250..20756)
                     /gene="tpx"
                     /locus_tag="ACHY03_001648"
     CDS             complement(20250..20756)
                     /gene="tpx"
                     /locus_tag="ACHY03_001648"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFS3904891.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            20875..21840
                     /gene="ycjG"
                     /locus_tag="ACHY03_001649"
     CDS             20875..21840
                     /gene="ycjG"
                     /locus_tag="ACHY03_001649"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFS3904892.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(21815..22543)
                     /gene="mpaA"
                     /locus_tag="ACHY03_001650"
     CDS             complement(21815..22543)
                     /gene="mpaA"
                     /locus_tag="ACHY03_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFS3904893.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(22834..23472)
                     /locus_tag="ACHY03_001651"
     CDS             complement(22834..23472)
                     /locus_tag="ACHY03_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3904894.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(23490..24422)
                     /locus_tag="ACHY03_001652"
     CDS             complement(23490..24422)
                     /locus_tag="ACHY03_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3904895.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            complement(24422..25342)
                     /locus_tag="ACHY03_001653"
     CDS             complement(24422..25342)
                     /locus_tag="ACHY03_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3904896.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQHPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            25480..26379
                     /gene="pgrR"
                     /locus_tag="ACHY03_001654"
     CDS             25480..26379
                     /gene="pgrR"
                     /locus_tag="ACHY03_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFS3904897.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAVNLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT         6491 a         6356 c         7078 g         6588 t
ORIGIN      
        1 aacgcagaaa tcaagaggac aatattatgg gtattttttc tcgctttgcc gacatcgtga
       61 atgccaacat caacgctctg ttagagaaag cggaagatcc acagaaactg gtgcgtctga
      121 tgatccagga gatggaagat acattggttg aagtacgttc tacttcggcg cgtgcgctgg
      181 cggaaaagaa acagctgact cgccgtattg aacaagcgtc ggcgcgtgaa gttgaatggc
      241 aggaaaaagc cgaactggca ctactgaaag agagagaaga tctggcgcgt gccgcgttaa
      301 ttgaaaaaca gaaactgacc gatctgatta agtccctgga acatgaagtg acgctggtgg
      361 acgatacgct ggtgcgtatg aagaaagaga tcggcgagct ggaaaacaaa ttgagcgaaa
      421 cccgtgctcg ccagcaggca ttgatgttac gccatcaggc ggcaaactcg tcgcgcgatg
      481 tgcgtcgtca gctggacagc ggcaaactgg atgaagcaat ggctcgtttc gaatctttcg
      541 aacgtcgaat tgaccagatg gaagcggaag cagaaagcca cagcttcggt aaacaaaaat
      601 cgctggacga tcagtttgcc gaactgaaag ccgatgatgc aatcagcgag caactggcac
      661 aattaaaagc caaaatgaag caagacaatc aataataaca tccaggcggc gtccgaacgc
      721 gccgccgctc atcgtctaag gagtacttat gagcgcgcta tttctggcta ttccgttaac
      781 catttttgtg ctgtttgttt taccgatctg gttatggctg cattacagca atcgttctgg
      841 tcgcagtgaa ttgtcgcaaa gtgagcagca gcgattagcg caactggctg atgaagcaaa
      901 acggatgcgc gaacgtattc aggcgctgga atctattctt gatgcagaac atccgaactg
      961 gagggatcgc taatggcggg cattaatctc aataaaaaat tatggcgtat tccacagcag
     1021 ggcatggtcc gtggcgtctg cgccgggatt gccaactatt ttgatgtacc ggtaaaactg
     1081 gtgcgtatcc tggtggtgtt gtcgattttc ttcggtctgg cgctatttac cctggttgct
     1141 tacatcattt tgtcatttgc gcttgatcca atgccggaca acatggcctt tggtgagcag
     1201 ctaccttcca gcagcgaatt gctggatgaa gtcgaccgtg aactggcggc aagtgaaacg
     1261 cgtttacgcg agatggaacg ttatgtcact tccgatactt tcacgttacg tagccgtttc
     1321 cgtcaactgt gaggaaagtt atgaatactc gctggcaaca ggccgggcaa aaggtaaagc
     1381 ctggtttcaa attagcaggc aagctggtac ttcttaccgc actgcgctat ggcccagcgg
     1441 gtgtggcggg ctgggcgata aaatcagttg ctcgccgacc gctgaaaatg ttgctggctg
     1501 tggcgctgga accgctgtta agtcgggctg ctaataaact ggcacagcgt tataaaaggt
     1561 gaggggagtt tcgcaaaaat tgttaaatct caggcgtata atggatagca atattcatcc
     1621 atagaaggac gcttacatgt ttaaaaaagg cttacttgct ctggcactgg tgttttcact
     1681 gcccgttttc gccgctgaac actggatcga tgttcgtgtt ccagagcagt atcagcaaga
     1741 gcacgttcag ggggccatca atattcccct gaaggaagtg aaagagcgca ttgccaccgt
     1801 cgttccggat aaaaacgaca ccgtgaaagt gtattgcaat gccggacgcc aatcagggca
     1861 ggcaaaagag atccttagcg agatgggata tacccacgtt gagaacgccg gtggcctgaa
     1921 agacatcgca atgccgaagg tcaaaggtta aaagaattca ccatgagcgg cgcttatgcg
     1981 ccgttttttt ctgtcacacc ttatttacat ccctatagat tatttgcgtc agctcacaaa
     2041 tacgcttttt ccctggtaaa aaatgatttc ctgagtgact aaaacccttg tgctcaattg
     2101 acagtttatt ttctgcggag tagtctctca tttcatggga ccgctaccac ggaaaggcaa
     2161 tatgaaacag aaaattacgg attacctgga cgaaatctac ggtggaacat ttaccgcaac
     2221 tcatttacag aaacttgtaa cgcgtcttga gagtgcgaaa cgattaatta cacagcgacg
     2281 taaaaaacac tgggatgaaa gtgatgtcgt gttaattacc tatgccgatc aatttcacag
     2341 taatgattta aaaccactac ccacatttaa tcagttttac catcaatggc tgcaaagcat
     2401 tttttcacat gttcatttat taccttttta tccatggtca tctgatgatg gattttcggt
     2461 aattgattat catcaggtcg ccagtgaagc gggggagtgg caggatattc agcagctcgg
     2521 tgaatgcagt catttaatgt ttgattttgt ctgcaaccat atgtcggcaa aaagtgaatg
     2581 gtttaaaaac tatttacaac agcagccagg ttttgaagat ttttttattg cggttgatcc
     2641 gcgaaccgat ctcagcgctg tcacccgtcc gcgtgcatta ccgctattaa cgccgtttca
     2701 aatggatgat aattccacgc gccatttatg gaccaccttt agcgacgatc aaattgacct
     2761 gaattaccgt agccctgacg tgttgctggc gatggtggat gttttgcttt gttacctgga
     2821 aaagggcgca gagtatgtcc gtctggatgc cgttggcttt atgtggaaag agccgggaac
     2881 aagctgcatc catctggaaa aaacacatct gattatcaaa ctgttacggt cgattattga
     2941 taacgttgcg ccaggtacag tgatcattac cgagaccaat gttccgcata aagacaacat
     3001 tgcttacttt ggcgaaggcg atgacgaagc gcatatggtg taccagttct cgctgccgcc
     3061 gctggtgtta catgcggtgc aaaaacagaa cgtcgaggcg ctgtgccagt gggcgcaaag
     3121 tctgagcttg ccctccggta aaacgacctg gtttaacttc ctcgcctctc acgatggcat
     3181 cgggctgaac ccactgcggg gcttgttacc agaaagcgaa atattagcgc tggtcgaggc
     3241 attacagcag gaaggggcat tagtaaactg gaaaaataat cccgacggta cgcgcagtcc
     3301 ctatgaaata aatgtcacct atatggatgc gttaagccgc cgcgagagta gcgatgaaga
     3361 acgttgcgcc aggtttatcc ttgcccatgc gattttgtta agtttccccg gtgtgccagc
     3421 gatatatatt caaagtattc tgggctcgcg taatgattac gcaggtgtcg aaaaactcgg
     3481 atataaccgt gcgattaacc gtaaaaaata ttacagagaa gagataaccc gagaactgaa
     3541 cgatgaagct acattaaggc atgcggtata tcatgagttg tcgcgtttaa ttatacttcg
     3601 tcgtagccat aacgagtttc atccggataa taattttacc atcgatacgg ttaattcatc
     3661 cgtaatgcgt attcaaagaa gtaacgcaga gggtaattgt ctgactggat tgtttaatgt
     3721 cagtaaaaat attcagcatg taaatattac tggcctgcac ggtcgggatc tgattagtga
     3781 agttgatata ttgggtaata aaataacgct gcgcccctgg caggttatgt ggattaaata
     3841 aaaaggaaca tctcatgatt aaatcaaaaa ttgtgctgtt atcagcactg gtttcctgcg
     3901 ccctgatttc aggctgtaaa gaagaaaata aaacccatgt atccatcgaa tttatgcatt
     3961 cttcggtgga gcaggagctc aggcagttat cagtaaattg attgcccgtt ttgaaaaaga
     4021 aaacccgggc atcaccatta agcaagtgcc cgtggaagaa gatgcctata acactaaagt
     4081 cattaccctt tcacgtagcg gttcgctgcc ggaagtgatc gaaaccagcc atgactacgc
     4141 caaagttatg gataaagagc agcttatcga tcgcaaagcg gttgccacgg tcatcagcaa
     4201 cgttggtgaa ggcgcgtttt acgatggcgt actgcgtatt gtgcgtaccg aagatggtag
     4261 cgcatggacc ggtgttcctg tcagcgcctg gattggcggc atctggtatc gcaaagatgt
     4321 gctggcaaaa gcggggctgg aggagccgaa aaactggcaa cagctgctgg acgttgcaca
     4381 gaaactgaat gacccggcga ataaaaaata cggtattgcg ctgcctacgg cagaaagcgt
     4441 gctgacggaa caatccttct cccagtttgc gttatccaac caggccaacg tctttaacgc
     4501 cgaaggcaaa atcacccttg atacaccaga gatgatgcag gcgctgacct attaccgcga
     4561 ccttgctgcc aacaccatgc cgggttctaa cgacatcatg gaagtgaaag acgcctttat
     4621 gaacggcacc gcgccgatgg cgatttactc cacctatatc cttccggctg tgattaaaga
     4681 aggcgacccg aaaaacgtcg gtttcgtggt gccaaccgag aaaaactctg cggtctacgg
     4741 catgttgacc tcgctgacca ttaccgccgg gcaaaagacc gaagagacgg aagcggcgga
     4801 aaaatttgtc acctttatgg aacaggcgga caacattgcc gactgggtga tgatgtcgcc
     4861 aggcgcggcg ctgccggtga acaaagcggt tgtcactacc gccacctgga aagacaacga
     4921 cgtgatcaag gcgctgggtg aactgccgaa tcagttaatt ggtgaactgc caaatattca
     4981 ggtctttggc gcagtagggg ataaaaactt tacccgcatg ggtgatgtga cgggttctgg
     5041 cgtggtgagt tcaatggtgc ataacgtcac cgtgggtaaa gccgatctct ctactacgct
     5101 gcaagcgagc cagaaaaaac tggatgaact gatcgaacag cactaaaccc aggacaggaa
     5161 tccgcaatga acaggctttt ttcaggtcgt tccgatatgc cgtttgcgct gctgcttctc
     5221 gcgcccagct tattactgct gggcggtctg gtggcgtggc cgatggtgtc gaatatcgaa
     5281 atcagttttt tacgtctgcc gctcaatccc aacatcgagt caacgtttgt tggggtgagc
     5341 aactatgtgc gtatcctctc cgatcccggc ttctggcatt cgctgtggat gacggtctgg
     5401 tataccgcgc tggtggtggc gggcagcacc gtgctcgggc tggcggtggc gatgtttttc
     5461 aaccgcgagt tccgcttgcg caaaaccgcg cgttcgctgg tgatcctctc ctacgtaacg
     5521 ccgtccattt cgctggtgtt cgcctggaaa tacatgttca acaacggcta cggcattgtg
     5581 aactacctcg gcgtcgatct tctgcatctc tacgagcagg caccgctgtg gttcgataat
     5641 ccgggcagta gctttgtgct ggtggtgctg ttcgccatct ggcgctattt cccatatgcc
     5701 tttatctcgt ttctggcgat tttgcagacc attgataaat cgttgtacga agcggcagag
     5761 atggatggcg ctaatgcctg gcagcggttt cgcatcgtca cgctgcccgc aattatgccg
     5821 gtcctggcga cggtggtgac cctgcgcacc atctggatgt tctacatgtt cgctgacgtt
     5881 tatctgctga cgaccaaggt cgatattctc ggtgtctatc tctacaaaac cgcctttgcc
     5941 tttaatgact tagggaaagc ggcggcgatc tcggtggtgc tcttcatcat tattttcgct
     6001 gtcattctgc tgaccagaaa acgggtgaac ctcaatggca acaaataaac gcacactcag
     6061 tcgcatcggt ttttactgcg ggctggcgct gtttctcatc atcacgctgt ttccgttttt
     6121 tgtgatgctg atgacctcgt tcaagagcgc aaaagaggcg atctcgctgc atcctacgct
     6181 gctgccgcaa cagtggacgc tggagcatta tgtcgacatt tttaacccgg tgattttccc
     6241 gtttgtcgac tacttccgta acagcatggt ggtgtcggtg gtttcatccg tggtggcggt
     6301 atttctcggt attctcgggg cttacgcgct ttctcgcctg cgctttaaag gtcggatgac
     6361 catcaacgcc agcttttaca cggtgtacat gttctccggc attttgctgg tggtgccgct
     6421 gttcaaaatt atcaccgcac ttggcattta cgacaccgag atggcgctga tcattaccat
     6481 ggtgacgcaa accctgccca ccgccgtgtt tatgctgaaa agctacttcg acaccatccc
     6541 tgatgagatc gaagaagcgg cgatgatgga tggcctcaac cgtctgcaaa ttatcttccg
     6601 tatcaccgtg cccctggcga tgtccgggct gatatcggtg ttcgtctact gctttatggt
     6661 ggcgtggaac gactatctgt ttgcgtcgat tttcctctcc agcgccagca atttcacctt
     6721 accagtgggc ctgaacgcgc tgttcagtac gcctgattac atctggggac gcatgatggc
     6781 ggcctcgctg gtgaccgcat tgccggtggt gattatgtat gcgctttcgg aacgttttat
     6841 taagagtggt ttgaccgccg gtggcgtgaa gggctaaagc ggccagtttc tttacaagga
     6901 gttttacatg aaaaagttag tagccacagc accgcgcgtt gctgcgctgg ttgagtatga
     6961 agatcgggcg attttagcca atgaagtgaa gatccgcgtg cgtttcggcg caccgaaaca
     7021 cggaacggaa gtggtcgact tccgcgccgc cagcccgttt atcgatgaag actttaacgg
     7081 cgagtggcag atgttcaccc cgcgtccggc agatgcgccg cgcggcattg agtttggcaa
     7141 attccagctt ggcaacatgg tggttggcga cattatcgag tgcggcagcg acgttaacga
     7201 ctacgcggtg ggcgacagcg tatgcggcta cggcccgctc tccgagacgg tcatcattaa
     7261 cgcagtgaat aactacaagc tgcgcaaaat gccggaaggc agctcctgga aaaacgccgt
     7321 ctgctacgac ccggcgcagt tcgccatgag tggcgttcgc gatgccaacg tacgcgtagg
     7381 ggattttgta gtggtggtag ggcttggcgc gatcggtcaa attgccatcc aactggctaa
     7441 acgcgctggc gcgtcggtgg taattggcgt cgatcctatt gcccatcgtt gtgatattgc
     7501 tcgtcgtcac ggtgcggatt tctgccttaa tcccattggc actgacgtag gcaaagagat
     7561 caaaacgctg accggcaagc agggtgccga tgtgattatc gaaaccagcg gttacgccga
     7621 cgcgctacag tcggcgctgc gcggcctggc ttacggtggc accatctcct atgtcgcgtt
     7681 tgccaaacca tttgctgaag gttttaacct cggacgcgaa gcgcatttca ataacgccaa
     7741 aattgtcttc tcccgcgcgt gcagcgaacc gaacccggat tatccgcgct ggagccgtaa
     7801 gcgtattgaa gaaacctgct gggaactgct gatgaacggt tatctaaatt gcgaagatct
     7861 aatcgacccg gtggtgacct ttgccaatag cccggaaagc tacatgcagt atgtcgacca
     7921 gcatccggaa cagagtatca aaatgggcgt cacgttttaa ttcaaaggaa tgaaaaaatg
     7981 aaaataggca cacagaatca ggcgttcttc ccggaaaata ttctggagaa atttcgttat
     8041 atcaaagaga tgggcttcga tggttttgag attgacggca aactgctggt caacaacatc
     8101 gaagaagtca aagcggcaat caaagaaacc ggtttaccgg tgaccaccgc ctgcggcggc
     8161 tatgacggct ggattggtga ctttatcgaa gagcgtcgtc ttaatggctt aaagcagatc
     8221 gaacgcattc tcgaagcgct ggcagaagtg gggggtaaag gtatcgtcgt tccggcagcg
     8281 tggggcatgt ttaccttccg cttaccgccg atgacctcgc cgcgtagcct ggacggcgat
     8341 cgtaaaatgg tgagcgattc cctgcgcgta ctggaacagg tcgccgcgcg taccggaacc
     8401 gtggtgtatc tcgaaccgtt aaaccgctat caggatcata tgatcaacac cctcgccgat
     8461 gctcgtcgtt acatcgtcga aaacaatctt aaacatgtgc agattatcgg cgatttctat
     8521 cacatgaaca tcgaagaaga taacctggcg caggcgctgc acgacaaccg tgacttgctc
     8581 ggtcatgtgc atattgctga taaccatcgt taccagccgg gcagcggcac cctcgatttc
     8641 cacgcgctgt ttgaacagct gcgcgccgat aactatcagg gttatgtggt gtatgaaggg
     8701 cgtatccggg cagaagatcc tgcccaggcg taccgtgatt cgttggcctg gttgcgtacc
     8761 tgctaagagg tctttgtgaa aagtgcaatg acaagctctc cgctgcgtgt cgcgataata
     8821 ggcgcaggcc aggtggcgga taaggttcat gcttcgtact actgcacccg caacgatctg
     8881 gaactggtgg ctgtctgtga cagccgcctt tcccaggcgc aggcgctggc agaaaaatac
     8941 gggaatgcat ccgtgtggga cgatccgcag gccatgctgc tggcggtgaa acctgatgtg
     9001 gttagcgtct gctcacctaa ccgttttcat tacgaacata ccctgatggc gcttgaagcg
     9061 ggctgccatg tgatgtgcga aaaaccgccc gccatgacgc cagaacaggc gcgggaaatg
     9121 tgcgataccg cgcgcaaaca gggcaaggtg ctggcctacg actttcatca tcgttttgca
     9181 ctcgatacgc aacagctgcg tgaacaggtg accaacggcg ttttgggaga gatttacgtt
     9241 accaccgccc gcgccctgcg tcgctgcggc gttcccggct ggggggtctt taccaataaa
     9301 gaattgcagg gcggcgggcc gcttattgac atcggcattc atatgctgga tgcggcgatg
     9361 tatgtactgg gctttccggc ggtgaaaagc gtgaatgcgc atagctttca aaagatcggc
     9421 acgcaaaaga gctgtggtca atttggcgag tgggatccgg caacttacag cgtcgaagat
     9481 tcgctgtttg gcaccattga atttcataac ggcggcattc tgtggctgga aacgtcattt
     9541 gcgctcaaca tccgcgaaca gtcgattatg aacgtcagct tttgtggtga taaagccggt
     9601 gcgacgctgt ttccagcgca tatctacacc gataacaacg gtgagttaat gactctgatg
     9661 caacgggaaa tggcggatga caaccgccat ttgcgcagca tggaagcctt tatcaatcac
     9721 gtacagggca agcccgtgat gatagccgac gccgagcagg ggtacatcat ccagcaactg
     9781 gtggcggcgt tgtatcaatc cgcagaaaca gggacgcgtg tggaattatg accaggccag
     9841 taacgttatc agaaccccat ttcagccagc ataccctgaa caagtatgca tcactgatgg
     9901 cgcaggggaa cggctatctt gggcttcgcg ccagccatga agaagattac acgcgccaga
     9961 cgcgcggcat gtatctggcg gggctttatc atcgggcggg taaaggtgaa atcaacgaac
    10021 tggtgaacct gcccgatgtg gtggggatgg agattgtcat aaatggtgag gttttctcat
    10081 tatcccgcga agcctggcag cgtgagcttg actttgccag tggcgaatta cggcgcagcg
    10141 ttgtctggtg taccagcaac ggcgcgcgtt acaccatcgc cagccgtcgc tttgtttcgg
    10201 cagaccaact gccgctcatt gcgctggaaa tcactattac gccactggac gctgacgcgt
    10261 cagtgctgat ttcaacaggt atcgacgcca cgcaaaccaa ccacggtcgc caacatctcg
    10321 acgaaaccca ggtgcgggtg tttggtcagc atctgatgca ggggatctac accacccagg
    10381 atggacgcag tgatgtggcc atcagctgtt gctgtaaggt gagcggtgat gtgcagcaat
    10441 gctataccgc caaagagcgc cgtttgctgc aacataccag tgcgcagctt catgcaggcg
    10501 agacagtgac gttgcaaaaa ctggtgtgga tcgactggcg ggatgacagg caagccgttt
    10561 tagacgagtg gggcagcgcg tcgcttcgcc agcttgaaat gtgcgcgcag cagagttacg
    10621 accaacttct tgcagcatca acagaaaact ggcgtcaatg gtggcagaaa cgtcgtatca
    10681 cagtaaatgg cggcgatgcg cacgatcagc aagcgttaga ttatgcgctt tatcatctgc
    10741 gcatcatgac gccggctcac gacgagcgca gcagtattgc ggcaaaaggc ttaaccggcg
    10801 aaggctacaa aggccacgtt ttctgggata cagaagtatt tttgctaccg tttcatctgt
    10861 ttagcgatcc gacggttgcc cgaagtttac tgcgttatcg ctggcacaac ttgcctggcg
    10921 cgcaggagaa agcgcgacgc aatggttggc agggcgcgct gtttccgtgg gagagcgcgc
    10981 gcagcggcga agaagagacg ccggaatttg ccgccattaa catccgcacc ggactgcggc
    11041 aaaaagtggc ctcggcgcag gctgaacatc atctggtcgc cgatatcgcc tgggctgtca
    11101 ttcaatactg gcaggcaacg ggagatgaaa gcttcatcgc tcacgaaggc atggcgctac
    11161 tgctggaaac tgcgaaattc tggattagcc gcgcggtgag agttaacgat cgtctggaaa
    11221 ttcatgatgt tattgggcca gacgaatata ccgaacatgt caataataac gcatacacca
    11281 gctatatggc ctgctacaac gtccaacagg cgctgaatat tgcccgccag ttcggctgta
    11341 gcgacgatgc gtttattcat cgtgccgaaa tattcctcaa agagttgtgg atgccagaga
    11401 ctcagcccga cggcgttttg ccgcaggatg attcgtttat ggctaagccg gcgattaacc
    11461 tggcgaaata caaagcggcg gcggggaagc aaaccattct gctggattat tcacgcgcag
    11521 aagtgaacga gatgcagatc ctcaaacaag ctgatgtggt gatgctcaat tacatgctgc
    11581 cggagcagtt ctcagcggta tcgtgtcttg ccaatctgca attttatgaa ccgcgcacca
    11641 ttcacgactc gtcattaagt aaagcaatcc acggcattgt tgccgcacgc tgtggcctgc
    11701 tgacccaaag ttatcagttc tggcgcgagg ggactgaaat cgatcttggt gctgatccgc
    11761 atagttgtga tgatggtatc cacgctgccg caactggcgc tatctggctg ggggcgattc
    11821 agggttttgc cggggtgagc gtgcgtgacg gtgaattgca tctcaatccg gcgttacctg
    11881 agcagtggca acagttgtct ttccctctgt tctggcaggg ctgcgaatta caggtcactc
    11941 tcgacgcgca gcgtattgcg attcgaactt ctgcgcccgt ttcactgcgt ttgaacggcc
    12001 agcttatatc cgtggctgaa gaatctgttt tctgtttggg tgattttatt ttgcccttca
    12061 atgggaccgc taccacgcat caggaggatg aatgaaactg caaggggtaa ttttcgatct
    12121 ggatggtgta atcaccgata ccgcgcatct gcatttccag gcgtggcagc agattgccgc
    12181 taaaattggc atcagcattg atgcgcagtt taacgaatcc ctaaaaggga ttagccgcga
    12241 tgagtctctg cggcgcattc tgcaacacgg gggcaaagag ggcgacttta acccgcagga
    12301 gcggacgcaa ctggcgtatc gcaaaaatct gctttacgtc cactcactgc gcgagctgac
    12361 ggttaatgct gtgctgcccg gtattcgcaa tttgctggca gagctccgtg cacagcagat
    12421 tccggtaggg ctggcttccg tctccctgaa tgcgccgacg attttagcgg cgctggagct
    12481 gcgcgagttt ttcaccttct gcgcggatgc ttcccaactt aaaaactcga aaccggatcc
    12541 ggaaatcttt ctcgctgctt gtgccgggct gggcgtgccg ccgcaggcat gtatcggcat
    12601 tgaagatgcg caggcgggga ttgacgccat taacgccagc ggtatgcgct cggtggggat
    12661 cggcgcgggc ttaaccgggg cgcaattact attgccttca acggactccc tgacctggtc
    12721 gcggttatcg gccttctggc aaaacgtata gcaaaggaat taacatggct cagctttcgt
    12781 tacaacatat tcaaaaaatc tacgataacc aggtgcatgt ggtgaaggac ttcaacctgg
    12841 aaattgccga taaagagttc attgtatttg tcggcccgtc gggctgcggt aaatcgacca
    12901 ccctgcgcat gattgccggg cttgaggaga tcagcggcgg cgatctgttg atcgacggca
    12961 aacgaatgaa tgacgttcca gccaaagcac gcaatatcgc aatggtgttc cagaactacg
    13021 cgttgtatcc gcatatgacg gtttacgaca acatggcgtt tggccttaag atgcaaaaaa
    13081 tcgccaaaga ggtgattgat gagcgggtga actgggcggc gcaaattctc ggcctgcgtg
    13141 agtacctgaa gcgcaagccg ggcgcgcttt ccggcgggca acgccagcgt gtggcgctcg
    13201 ggcgggcgat tgtgcgcgaa gcgggcgtgt ttttaatgga tgaaccgctc tctaaccttg
    13261 atgccaagct gcgcgtacaa atgcgcgcag agatcagcaa gctgcatcag aaactgaaca
    13321 ccaccatgat ctacgtgacc cacgatcaga ccgaagcgat gaccatggcg acgcggattg
    13381 tgattatgaa ggacgggatt gttcagcaag taggtgcgcc gaaaatggtt tataaccaac
    13441 ccgcgaatat gttcgtagcc ggatttattg gatcgcctgc gatgaatttt attcgcggca
    13501 cgatcgatgg cgataaattc gttacggaaa cgcttaaatt aaccataccc gaagagaaat
    13561 tagcggttct gaaaacacag gaaagtttgc ataagcccat cgtgatggga atacgcccgg
    13621 aagatattca tccggacgcg caagaagaaa ataacatttc cgccaaaatt agcgtggcag
    13681 aattaaccgg tgcggaattt atgctctaca ccacggttgg ggggcacgag ttagtggtcc
    13741 gtgctggtgc gttaaatgat tatcatgcag gagaaaatat caccattcat tttgatatga
    13801 cgaaatgtca tttctttgat gcagaaacgg aaatagcaat tcgctaaata cagggggaag
    13861 gcattccccc aggataatac aaggaacaat aatgaaaaag ttattaccct gtaccgcact
    13921 ggtgatgtgt gcgggaatgg cctgcgcaca ggccgaggaa aagaacgact ggcactttaa
    13981 tatcggcgcg atgtacgaaa tagaaaacgt cgagggttat ggcgaagata tggatgggct
    14041 ggcggagcct tctgtctact ttaatgccgc caacgggccg tggagaattt ctctggccta
    14101 ttatcaggaa gggccggtag attatagcgc gggtaaacgt ggaacgtggt ttgatcgccc
    14161 ggagctggag gtacattatc agttcctcga aagcgatgat ttcagtttcg gcctgaccgg
    14221 cggtttccgt aattatggtt atcactacgt tgatgaaccg ggtaaagaca cggcgaatat
    14281 gcagcgctgg aaaatcgcgc cagactggga tgtgaaactg actgacgatt tacgtttcaa
    14341 cggttggttg tcgatgtata aatttgccaa cgatttgaac acgaccggtt acgctgatac
    14401 ccgtgtcgaa acggaaacag gtctgcaata taccttcaac gaaacggttg ctttgcgagt
    14461 gaactattat ctcgagcgcg gcttcaatat ggacgacagc cgcaataacg gtgagttttc
    14521 cacgcaagaa attcgcgcct atttgccgct gacgctcggc aatcactcgg tgacgccgta
    14581 tacgcgcatt gggctggatc gctggagtaa ctgggactgg caggatgata ttgaacgtga
    14641 aggccatgat tttaaccgtg ttggtttatt ttacggttat gatttccaga atggtctttc
    14701 cgtttcgctg gaatacgcgt ttgagtggca ggatcacgac gaaggcgaca gcgataaatt
    14761 ccattatgca ggtgtcggcg taaattactc gttctgataa tgggctaaat tgccggatgc
    14821 ggcgcgagta ctttatccga tctataaatg taggccggat aagatgcgcc agcatcgcat
    14881 ccggcattca ggcaaggtcg ctggtattta tttcagcgtc atatgcgtgg caacggtaat
    14941 attctgtggt gacggttttc cagaaattaa gcggaataat aactcgcagc tttgttgacc
    15001 taactcctgc gtcggaacat cgatgccgcc cggtgcaggc gttaaaataa atgacagcgt
    15061 ttcattgcta taacccacca ccgctaactg ctgcggaata gcaatatttt tctctgctgc
    15121 cgcacgataa atgctcatta atttcaggct gtcagtggca aacaccgcct caggcaacgg
    15181 tgactggctt aataattctc tcgcggcttg cagtgctgtt tcatgggtat aaccgccgtc
    15241 aacaatccat tcatcacgca ctgcaatatt atgcgcggac agactttgct tataaccatt
    15301 tacccgatcc actgaaacat ggacatcaag cggagcatgc aggcaggcaa tattttgatg
    15361 cccactttca attaacgcat cggtcaacgc aatgctgtca ccaaaattat cggtatcgac
    15421 agaataaaca tgggcatatt gaccttcaac tttgccaatc accacgacag gaatatcgta
    15481 tttatcaagt tgggcaaaaa atgactcatc cgctggcgaa cttagcataa taatgccttt
    15541 aatcattttc tgcttaattt tgctttcaca tttttgtaag tcttctgcca ggttgtgcga
    15601 cgtctgcaat atcacgtcga aaccttcttc ttccgctttg gcggtgatgg catgtagaac
    15661 ttcagagaaa aacggattac ccgccgtagt tttggtcgaa cgggtagaaa tcaccataat
    15721 ggcatcaaaa cccgaagagg tcagcgcgcg ggccagcttg tttggctgat actgtaattc
    15781 ttcaatggcc cgtaacactt tttcgcgcgc ttccggggag atattagttt gcttattcag
    15841 cacgcgtgat acggtggatt ttgatacgcc tgcaaccctg gcaatatcat aaatagtagg
    15901 ggacataggt caggaactcc gtccgaacgt gaatgctgca catcttatgg agttcgcccg
    15961 tcgatgacaa cacgggtagt tgataatcaa tggcctggcc cccacattca tatccttatg
    16021 aatgattttt tttctggtct tcaaagcagc acaggacagc aatgaagcga cttaaaaatg
    16081 aactcaatgc gctggtgaat cggggtgtcg acagacatct gcgcctcgct gtaaccggac
    16141 ttagccgcag cggcaaaaca gcatttatca ccgcgatggt taatcagttg cttaatattc
    16201 atgccggagc acgcttgccg ctgttaagtg cggtgcgtga agagcgcttg ctgggcgtaa
    16261 aacgcattcc ccagcgtgac tttggcattc cgcgcttcac ctatgatgaa ggactggcgc
    16321 agttatatgg cgatccacct gcctggccaa cgccaacgcg cggcgtcagc gaaattcgcc
    16381 tggcgctacg ttttaaatcg aacgattcgc tgctacgcca ctttaaggat acctccacgc
    16441 tgtatctgga gattgtggat tatcccggcg aatggttgct cgacctgccg atgctggcgc
    16501 aggactattt aagctggtcg cgccagatga cgggcttact taatggtcag cgcggcgaat
    16561 ggtcggcgaa atggcgaatg atgtgcgaag ggctggaccc gctagcgcct gccgacgaaa
    16621 accggctggc agacattgcc gccgcgtgga ccgattatct ccaccactgt aaacagcagg
    16681 ggctgcactt tattcagcct gggcgctttg ttttgccggg agatatggca ggtgcgcccg
    16741 cgctgcaatt cttcccgtgg ccggatgtcg atgcctgggg cgagtccaaa ctggcgcagg
    16801 ccgataagca caccaatgcc ggaatgctgc gcgagcggtt taattattac tgcgagaaag
    16861 tggtgaaggg gttctataag aatcattttc tgcgctttga ccgccagatt gtgctggtgg
    16921 attgcctgca acctctcaac agtgggccac aggcatttaa tgatatgcgt ctggcgctga
    16981 cgcagctgat gcaaagtttc cactacgggc agcgtactct gttcaggcgt ttgttttcgc
    17041 cggttatcga taagctattg tttgctgcca ctaaagcgga ccatgtgacc atcgatcagc
    17101 acgccaatat ggtttcattg ctgcaacaac tgattcagga tgcctggcaa aatgcggcgt
    17161 ttgaagggat tagtatggat tgcctggggc tggcctcggt gcaggcgacc accagcggca
    17221 ttattgatgt caacggcgag aaaattccgg cattgcgcgg taaccggctc agcgatggtg
    17281 cgcctctcac cgtttacccc ggtgaagttc ctgcgcgttt gcccggtcag gcgttctggg
    17341 acaagcaagg gttccagttt gaagcgtttc gcccgcaggt gatggatgtc gacaaaccgc
    17401 tgccgcatat tcgtcttgat gccgcgctgg aatttttaat aggagataaa ttgcgatgac
    17461 cgaaccgtta aaaccacgta ttgatttcga cggtccgctg gaggtcgatc agaatccaaa
    17521 attcagggcg cagcagacct ttgacgaaaa tcaggcgcaa aattttgccc cggccacgct
    17581 cgacgaagcg caggaagaag aggggcaagt cgaagcggta atggacgcag cgttacgtcc
    17641 gaaacgcagc ctgtggcgca aaatggtgat gggcgggctg gctctgtttg gcgcaagcgt
    17701 tgtcgggcag ggtgtacagt ggacaatgaa tgcctggcaa actcaggact gggtggcgct
    17761 gggtggatgt gccgctgggg cattgattat cggcgctggc gtaggttctg tggtaacaga
    17821 gtggcggcgc ttatggcgct tgcgacagcg cgcccatgaa cgcgacgaag cgcgtgattt
    17881 attgcatagc cacggaaccg gtaaaggccg cgcattttgc gaaaaactgg cgcagcaggc
    17941 gggcatcgat cagtcacatc cggcgctgca acgctggtat gcctcaatcc atgaaacgca
    18001 aaacgaccgt gaagtggtca gtttgtatgc gcatttggtc cagccggttt tagatgccca
    18061 ggcgcggcgc gaaatcagcc gttcggcagc ggaatcaacg ttgatgattg cggtcagccc
    18121 gctggcgctg gtggatatgg catttatcgc ctggcgcaat ctgcgtttga ttaatcgcat
    18181 cgccacgctg tatggcattg aactggggta ttacagccgt ttgcgcctgt tcaagctggt
    18241 attgctgaat atcgcttttg ccggagccag cgaactggtg cgcgaagtgg ggatggactg
    18301 gatgtcgcaa gatctcgctg ctcgtttgtc tacccgcgca gctcagggaa ttggtgctgg
    18361 acttctgacg gcacgactcg ggattaaagc tatggagctt tgccgcccgc tgccgtggat
    18421 tgatgatgac aaacctcgcc tcggggattt ccgtcgtcag cttatcggtc aggtaaaaga
    18481 aacgctgcaa aaaggcaaaa cgcccagcga aaaataatgc aatatcgggt gctgaccgga
    18541 tatctttacg ccgaagtgcc cgtttttccg tctttgtgtc aatgattgtt gacagaaacc
    18601 ttcctgctat ccaaatagtg tcatatcatc atattaattg ttcttttttc aggtgaaggt
    18661 tcccatgcgt ctggaagtct tttgtgaaga ccgactcggt ctgacccgcg aattactcga
    18721 tctactcgtg ctaagaggca ttgatttacg cggtattgag attgatccca ttgggcgaat
    18781 ctacctcaat tttgctgaac tggagtttga gagtttcagc agtctgatgg ccgaaatacg
    18841 ccgtattgca ggtgttaccg atgtgcgtac tgtcccgtgg atgccttccg aacgtgagca
    18901 tctggcgttg agcgcgttac tggaggcgtt gcctgaacct gtgctctctg tcgatatgaa
    18961 aagcaaagtg gatatggcga acccggcgag ctgtcagctt tttgggcaaa aattggatcg
    19021 cctgcgcaac cataccgccg cacaattgat taacggcttt aattttttac gttggctgga
    19081 aagcgaaccg caagattcgc ataacgagca tgtcgttatt aatgggcaga atttcctgat
    19141 ggagattacg cctgtttatc ttcaggatga aaatgatcaa cacgtcctga ccggtgcggt
    19201 ggtgatgttg cgatcaacga ttcgtatggg ccgccagttg caaaatgtcg ccgcccagga
    19261 cgtcagcgcc ttcagtcaaa ttgtcgccgt cagcccgaaa atgaagcacg ttgtcgaaca
    19321 ggcgcagaaa ctggcgatgc taagcgcgcc gctgctgatt acgggtgaca caggtacagg
    19381 taaagatctc tttgcctacg cctgccatca ggcaagcccc agagcgggca aaccttacct
    19441 ggcgctgaac tgtgcgtcta taccggaaga tgcggtcgag agtgaactgt ttggtcatgc
    19501 tccggaaggg aagaaaggat tctttgagca ggcgaacggt ggttcggtgc tgttggatga
    19561 aataggtgag atgtcaccgc ggatgcaggc gaaattattg cgtttcctta atgatggtac
    19621 tttccgtcgg gttggtgaag accatgaggt gcatgtcgat gtgcgggtga tttgcgctac
    19681 gcagaagaat ctggtcgaac tggtgcaaaa aggcatgttc cgtgaagatc tctattatcg
    19741 tctgaacgtg ttgacgctca atctgccgcc gctacgtgac tgtccgcagg acatcatgcc
    19801 gttaactgag ctgttcgtcg cccgctttgc cgacgagcag ggcgtgccgc gtccgaaact
    19861 ggccgctgac ctgaatactg tacttacgcg ttatgcgtgg ccgggaaatg tgcggcagtt
    19921 aaagaacgct atctatcgcg cactgacaca actggacggt tatgagctgc gtccacagga
    19981 tattttgttg ccggattatg acgccgcaac ggtagccgtg ggcgaagatg cgatggaagg
    20041 ttcgctggac gaaatcacca gccgttttga acgctcggta ttaacccagc tttatcgcaa
    20101 ttatcccagc acgcgcaaac tggcaaaacg tctcggcgtt tcacataccg cgattgccaa
    20161 taagttgcgg gaatatggct taagccagaa gaagaacgaa gagtaagcgc gaatatgcct
    20221 gatggtgcaa caccatcagg catattaact tatgctttca gtacagccag agctgcttcg
    20281 taatccggct cggtggtgat ttcatccacc agctggctga aaatcacatt gtcattttca
    20341 tcaataacca caacggcacg cgctgccaga cctttcagtg ggccatcagc aattgccaca
    20401 ccgtaggctt gcagaaattc agcgttacgg aaagtggaga gggtgataac gttgttcaga
    20461 ccttctgcgc cgcagaaacg agactgggcg aacggcagat cggcagagat acacagcaca
    20521 acggtgttgt cgatctcggt tgccagttgg ttaaacttac gtactgatgc ggcgcaaaca
    20581 ccggtatcaa tactcgggaa aatgttcagc actttgcgtt tacccgcaaa ctgaccgagg
    20641 gtgacgtcag acagatcttt tgccacgaga gtaaaagtct gcgctttgct acccgcctgc
    20701 gggatggagt tggcgactgt aaccgggttg ccctggaaat gaacggtttg tgacatgatt
    20761 atctttcctg tttacatata gttaacgtca catctagttt atgctaactg tcaataacac
    20821 agcaaacgct atttgcgctt aatccgcaga ccaccgcgac aacaaggagt aaagatgaga
    20881 accgttaagg tattcgagga agcctggccc ttacataccc cgtttgtgat tgcccgggga
    20941 agtcgcagtg aagcgcgcgt ggtggtggtt gaactggaag aagagggtat taaaggcacc
    21001 ggcgaatgca cgccgtatcc gcgttatgga gaaagtgatg cctcggtaat ggcgcaaatt
    21061 atgagcgtcg taccgcaatt agagaaaggg ctgacacggg aggagttgca aaaaattctc
    21121 cctgccggcg cagcacgtaa tgcgctggat tgtgcattgt gggatctggc ggcgcgaaaa
    21181 cagcagcaat cgctggctga tttgatcggc ataacgcttc ccgggacagt caccactgca
    21241 cagacggtag tcatcggtac gcctgatcag atggccaata gcgcatcaac actctggcag
    21301 gccggcgcga aattattgaa agtgaagctg gataaccatc ttatcagtga gcggatggtg
    21361 gcaattcgca cagctgtgcc cgatgccacg ctgatcgttg atgcaaatga atcctggcgt
    21421 gcagaagggt tggcggcacg ttgccagcta ttggcggatt taggcgttgc tatgcttgaa
    21481 caaccgcttc ctgcgcagga cgatgcggca ctggagaatt ttattcatcc gttaccgatt
    21541 tgtgctgatg aaagttgtca tactcgtagc aatctgaagg cgctgaaagg gcgctatgag
    21601 atggttaaca ttaagctcga taaaaccggg ggtctgacgg aagcgctggc gctggcgact
    21661 gaagcgcgtg cacaaggttt tcgtctgatg ctgggctgca tgttgtgtac ctctcgggcc
    21721 attagcgccg ctttaccgct ggtgccgcag gtcagtttcg ccgatcttga cggaccgacc
    21781 tggctggcgg tagatgtgga accggcgctt cagttcacga cgggcgaatt gcatctttag
    21841 gatgccagcg cagcaagttt gccatcgcaa agagatattt ttcgctggct tcgtcggagg
    21901 agatgggcgg aaactctgcg gtgatgcaat gcaagttgag atctgcacac caactgccga
    21961 aagagccagg cgtttcataa ccgacgctgg ttaccagagg caattcaaac gcctgagcca
    22021 gccattcgcc taattcgctg tgtctgggat cttcaataca ggccagtgga tcgtggaatg
    22081 agaccaccca ggcaggttga atgcggtgta taagctggca cagcgcctgg gtttcaggtt
    22141 cggagccagg tttgtcacct gtcagtaata cgacatcacg ttcttcagcg gcgctgttcc
    22201 agcgataaac cgtttcacct tccttccagt tcgccgccgg aaagtttctg tttaaatcca
    22261 caccattagc attggcccgt aaccccagct ggcagccgtc aggattcaca cacagcacca
    22321 catgatggcg gcgcaaagaa ggtgtcaaag tccgcagagc acaggagagc gtgacgaccg
    22381 aagagttttc atcaccgtga gtgccagcca gaatcaaacc actttcgcga ctggctgcag
    22441 gggccggaaa ccagattaac ggcgcgccca ataatgaacg tccgtaatgt tctgttccgg
    22501 gcggaaatgc gccgcgttcg gcgcgtgggc gggttacggt catgatcgtc tctggttcag
    22561 gaagtattac aggcagtgtt gtgcaaattt tgcggattat caaatccttt tcttcaacgc
    22621 tatgtcatcg agcttgtatc acttttttgc cggaagttgt ttgcattttc attgatgtgc
    22681 agatattaga attgtcgtaa tgttatatac atggcgtatt ttattcttac tggttctaca
    22741 catgttaatc gcatctgatt gatcagttca ccttaataag tttaataaat cgtactgcac
    22801 atagtacagt acgatggtag ctatataatt gatttacatg aagaatggct tatcgccatc
    22861 agtcccgggt tgattgatgc ctatattctc accaatgtgt ttctcaggtt tatcgatgat
    22921 atctgtaatt aatgctgcga cactcttacg tgatacgatc gtacctttga atggttcatt
    22981 gcgtgaggta agctcataat caataatatc ttcatccgtc agccaggcag ggcgaagaat
    23041 tgtatattcc agtccagaag cctcaattgc atccgcagcc ctgcggaatg gttttagggg
    23101 ttcaccaatc actgcattat tccattcgcc aaattttcct ggaacttcat cataaatacc
    23161 gagggaaaga acaaagatca gacgtttaac atcacaggct ttcatcgcag cgatcactga
    23221 atttgcctga atatctaaat cttcccccgt aaggttggca tacacaatat cctgtccctg
    23281 catggcctgc ttcagtgctg catgattcag tacatccccc ataataatct ggctattagt
    23341 tggatagggt ttgtgtattt ttgctggctg acgagcaaat aaagtttgtt taatggtttg
    23401 tttatctgcc agttgattaa tcacatggcg ggctatctga ccgccagcac caagaatgag
    23461 tacatttttc ataatatttc tcatgaaact tatcccagat gggtgttgaa aaaaggaata
    23521 actttttcca gcgcctcctg aactgcttcc ggtttgtcat acagttcata atgtgagaaa
    23581 tcgggtaatg aaactaactg acgatcctgc gaaaccatcg cccggccata gacttccagt
    23641 ccatcacgat aagcaccgaa ggcaccgact ttttcaccaa ctactaccat caccggctga
    23701 gtcagtaaca cttcagtaaa tgcgaaggca tcccaggcca gcgttttctg tgcatgtgag
    23761 aacaacattt ttgtcgcacc tcccggttgt tgcccgcgtg gtgttttata gtagtcagtt
    23821 gcttcataaa catcgcgttc ggtcagtcca tgggcttttg ctgcatccag gctggcggga
    23881 agtaattcgt tgatctgtaa ttctccacca cgagcctctt tggttcgttg cgacgccatt
    23941 gcattcaatg cgccaatcgg atcgtaattg ctgaaacctt cccgaaaaag acgcccgata
    24001 ttaacacccg taatactcac tacagcttta attcgttttt ctgtcagcgc ggcattaatg
    24061 gcatagccac cccccccaca cacacccagc acgccaatgc gttcagcatc aacatatggc
    24121 agagttactg cataatcgat aacacggcta atatcctcta cgcgctgtgt cggatcttct
    24181 atccaacgag gctccccacc agattctccc tggaaactgg cgtcataagc gagaacgaca
    24241 tagccttttt cagccaatgc cttgccatag atattgccag acgtctgctc cttacaactg
    24301 ccaaaaggat ggacgctgat aatcgccgga tactgttttg actcatcaaa atttggtgga
    24361 aactgaatat tggcagcaat atgccaggcc atatcactat tgcggatttg aatatgtttc
    24421 attacagagt ttcctcaaag aacggagcta atactgaaat tgcttcagcc acataggctt
    24481 tgccgtcata aagatccata tggtttgcgc cttcaacaat gtgatagcgt ttatcctggc
    24541 ttgatgctcg atcgtacagg tcgtcactca tccatttgct ccctgcctgg ctgcccgcca
    24601 caatctgcgt cggctgagtc aggtacactt ccgccatatg gtaagcatca taggtaataa
    24661 tctggttaag gctgcgcaaa gtagcgtaac ccggtgctgt tggatactgc gcgcgagggg
    24721 tgtggtaata ctcccaggcc tgacgcagtt cttcgttcgg cgcatcggac tccttcattg
    24781 gtgccagtgg cataatggca tattctccgc tgctaatatc gctggttcgg gcgtttgaac
    24841 ccgcgtcaac gtatggcagg gcatcaatag atttcacatt gttttcccaa ccattacgga
    24901 acatcgaacc aatattgacc gcactaacgg taccgatggc cttgatgcgg cgatcctgaa
    24961 ttgcagcatt ggctgtatat cctgcaccgg cacaaattcc catcgcacca attcgggtat
    25021 tgtcgacata agaaagcgtt gtcaggtaat caatcacggc actgatgtct tcagtacgaa
    25081 tgtatgggtt ttctaactga cgcggctcgc cgccactttc accttgataa gatgcgtcat
    25141 aagcgatagt gacaaatccc ttttccgcca gtttttcggc ataagttccg gccgtttgtt
    25201 ctttaacgcc cccacctggg tgagatacca caattgccgg atgctgacgg gtttcatcaa
    25261 attttggagg gaaatagatc actgcagaca aagagatggt tggattattg ctgttagtga
    25321 agctgacttt attattcatc attccgttcc tcatggagtt gtcggttcgt tttaacggtt
    25381 ggtgatatga ctatagatat tgatcattaa gttgattaga cccaaaatca tgattagacc
    25441 tatcatttaa atgattaata gattgtatgg agatactgaa tgaagcgtga agaaatcgct
    25501 gatctgatgg cgtttgtcgt cgttgcagag gagcgtagct tcactcgcgc agcagcccgc
    25561 ctgagcatgg cgcagtcagc tttaagccag atagtgcgcc gtatagaaga acgattggga
    25621 ttgcggcttc tgacgcgaac tacgcgcagc gttgtaccaa ctgaagcggg cgagcatctt
    25681 ttgtctgttc ttggccctat gttgcatgac atagattctg ccctgacatc cctgagcgat
    25741 ctgcagaacc gcccatccgg gacaatacgt attactactg tagaacatgc agcaaaaacg
    25801 atattgttac cagcaatgcg cacattcctg aaatcgcatc ctgaaattga tattcagctc
    25861 accattgatt atggtttgac cgatgtcgtt tctgaacgtt ttgatgcagg cgtccgtctg
    25921 ggtggggaga tggataaaga tatgatcgcc attcgaatcg ggccagatat accaatggct
    25981 attgttggct caccggatta tttttctcgc cgaagtgttc caacgtcagt gtcacaatta
    26041 atagatcatc aggcagttaa tttgtatctt cccacatcgg gtacagcaaa tcgctggaga
    26101 ttaatacgcg gtggacgcga agttcgtgtt cgcatggaag gtcagctttt actgaatacg
    26161 atagacctga tcattgatgc tgcaattgat gggcatggat tggcgtatct accttatgat
    26221 caggttgagc gggctattaa agaaaaaaaa ctgatacgtg ttttggataa attcacacca
    26281 gatttacccg gttatcacct gtactatcca caccgtcgac atgctggctc ggcattctca
    26341 ttatttatag ataggctgaa gtataaaggt gctgtttagc actacttgct gatacattaa
    26401 tttaattctt ctcttaacgt attctcagtt cctttcaacg ttttggttat tttttattct
    26461 tcgtacaatg gcgacagatg ctgattatga taccgaaaac tggtttgaac gtg
//
LOCUS       DAZUCE010000047        26341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZUCE010000047 DAZUCE010000000
VERSION     DAZUCE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.047"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            3..980
                     /gene="tehA"
                     /locus_tag="ACHY03_001655"
     CDS             3..980
                     /gene="tehA"
                     /locus_tag="ACHY03_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFS3904898.1"
                     /translation="MLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGMVILAM
                     IIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFRPLAVC
                     LFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALGYTDAG
                     LVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSVNGGEG
                     DTLAKLLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGSGSDNG
                     FFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            977..1570
                     /gene="tehB"
                     /locus_tag="ACHY03_001656"
     CDS             977..1570
                     /gene="tehB"
                     /locus_tag="ACHY03_001656"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFS3904899.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFAGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGSYNLIVAAMDTADYPCTVGFPFAFKERELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            1873..2541
                     /gene="ydcL"
                     /locus_tag="ACHY03_001657"
     CDS             1873..2541
                     /gene="ydcL"
                     /locus_tag="ACHY03_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFS3904900.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(2559..3065)
                     /gene="tnpA"
                     /locus_tag="ACHY03_001658"
                     /pseudo
     CDS             complement(2559..3065)
                     /gene="tnpA"
                     /locus_tag="ACHY03_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            3073..4281
                     /gene="insQ"
                     /locus_tag="ACHY03_001659"
                     /pseudo
     CDS             3073..4281
                     /gene="insQ"
                     /locus_tag="ACHY03_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            complement(4321..5535)
                     /gene="ydcO"
                     /locus_tag="ACHY03_001660"
     CDS             complement(4321..5535)
                     /gene="ydcO"
                     /locus_tag="ACHY03_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFS3904901.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGASTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            5588..6124
                     /gene="sutR"
                     /locus_tag="ACHY03_001661"
     CDS             5588..6124
                     /gene="sutR"
                     /locus_tag="ACHY03_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFS3904902.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            6197..8158
                     /gene="rlhA"
                     /locus_tag="ACHY03_001662"
     CDS             6197..8158
                     /gene="rlhA"
                     /locus_tag="ACHY03_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFS3904903.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTDPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWLEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(8250..8480)
                     /gene="yncJ"
                     /locus_tag="ACHY03_001663"
     CDS             complement(8250..8480)
                     /gene="yncJ"
                     /locus_tag="ACHY03_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFS3904904.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            8702..8878
                     /gene="hicA"
                     /locus_tag="ACHY03_001664"
     CDS             8702..8878
                     /gene="hicA"
                     /locus_tag="ACHY03_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFS3904905.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            8924..9340
                     /gene="hicB"
                     /locus_tag="ACHY03_001665"
     CDS             8924..9340
                     /gene="hicB"
                     /locus_tag="ACHY03_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFS3904906.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALQ
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            9419..10825
                     /gene="ydcR"
                     /locus_tag="ACHY03_001666"
     CDS             9419..10825
                     /gene="ydcR"
                     /locus_tag="ACHY03_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS3904907.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKVPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            11070..12215
                     /gene="ydcS"
                     /locus_tag="ACHY03_001667"
     CDS             11070..12215
                     /gene="ydcS"
                     /locus_tag="ACHY03_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFS3904908.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            12233..13246
                     /gene="ydcT"
                     /locus_tag="ACHY03_001668"
     CDS             12233..13246
                     /gene="ydcT"
                     /locus_tag="ACHY03_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3904909.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            13247..14188
                     /gene="ydcU"
                     /locus_tag="ACHY03_001669"
     CDS             13247..14188
                     /gene="ydcU"
                     /locus_tag="ACHY03_001669"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3904910.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            14178..14972
                     /gene="ydcV"
                     /locus_tag="ACHY03_001670"
     CDS             14178..14972
                     /gene="ydcV"
                     /locus_tag="ACHY03_001670"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3904911.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            14994..16418
                     /gene="patD"
                     /locus_tag="ACHY03_001671"
     CDS             14994..16418
                     /gene="patD"
                     /locus_tag="ACHY03_001671"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFS3904912.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(16515..16610)
                     /gene="yncL"
                     /locus_tag="ACHY03_001672"
     CDS             complement(16515..16610)
                     /gene="yncL"
                     /locus_tag="ACHY03_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFS3904913.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            16805..16978
                     /gene="ortT"
                     /locus_tag="ACHY03_001673"
     CDS             16805..16978
                     /gene="ortT"
                     /locus_tag="ACHY03_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFS3904914.1"
                     /translation="MSLYQHMLVFYAVMAAISFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            17064..17297
                     /gene="ydcY"
                     /locus_tag="ACHY03_001674"
     CDS             17064..17297
                     /gene="ydcY"
                     /locus_tag="ACHY03_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFS3904915.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(17298..17747)
                     /gene="ydcZ"
                     /locus_tag="ACHY03_001675"
     CDS             complement(17298..17747)
                     /gene="ydcZ"
                     /locus_tag="ACHY03_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS3904916.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(17744..18262)
                     /gene="mddA"
                     /locus_tag="ACHY03_001676"
     CDS             complement(17744..18262)
                     /gene="mddA"
                     /locus_tag="ACHY03_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFS3904917.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            18443..19480
                     /gene="curA"
                     /locus_tag="ACHY03_001677"
     CDS             18443..19480
                     /gene="curA"
                     /locus_tag="ACHY03_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFS3904918.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQSGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            19678..20343
                     /gene="mcbR"
                     /locus_tag="ACHY03_001678"
     CDS             19678..20343
                     /gene="mcbR"
                     /locus_tag="ACHY03_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFS3904919.1"
                     /translation="MPGTEKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(20379..22481)
                     /gene="pqqU"
                     /locus_tag="ACHY03_001679"
     CDS             complement(20379..22481)
                     /gene="pqqU"
                     /locus_tag="ACHY03_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFS3904920.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVTNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQSGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYITPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGVNIAWRFE"
     gene            22723..23784
                     /gene="yncE"
                     /locus_tag="ACHY03_001680"
     CDS             22723..23784
                     /gene="yncE"
                     /locus_tag="ACHY03_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFS3904921.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVMKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(23897..25396)
                     /gene="ansP"
                     /locus_tag="ACHY03_001681"
     CDS             complement(23897..25396)
                     /gene="ansP"
                     /locus_tag="ACHY03_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFS3904922.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            25663..26280
                     /gene="yncG"
                     /locus_tag="ACHY03_001682"
     CDS             25663..26280
                     /gene="yncG"
                     /locus_tag="ACHY03_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFS3904923.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLERRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTPEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNVII"
BASE COUNT         6706 a         6410 c         6778 g         6447 t
ORIGIN      
        1 aagtgctcaa tttgccggca ggctactttg gtattgtgtt ggggacgata gggatgggat
       61 ttgcctggcg ctatgccagc caggtttggc aggtcagcca ctggttaggg gatggaatgg
      121 tgattctggc gatgatcatc tggggattat taactagcgc atttattacc cgactcatac
      181 gctttccgca tagcgtgctg gcagaagtgc gccatccagt gctgagcagt tttgtgagtt
      241 tgtttccggc aacgacgatg ctggtagcga ttggttttgt tccgtggttt cgcccactgg
      301 cggtgtgcct gttcagtttt ggtgtcgtgg ttcagttggc ttatgccgcc tggcaaactg
      361 cgggattatg gcgcggatct caccctgaag aagctaccac gcctggactg tatctgccga
      421 cagttgccaa caactttatc agcgcaatgg cctgtggtgc gttgggctac accgacgccg
      481 gtctggtgtt tttaggcgca ggcgttttct catggctaag ccttgaaccg gtgatcttgc
      541 agcgtctgcg cagttcggga gaattaccca cggcactgcg gacatcactc ggcattcagc
      601 tcgctcctgc gctggtggcc tgtagtgcct ggctgagcgt caacggcggc gagggtgaca
      661 cgctggcgaa attgcttttc ggttatggac tgctgcaact gctgtttatg ctacgtctga
      721 tgccatggta tctctcccag ccatttaatg cttcattctg gagtttctcg ttcggcgtat
      781 ctgcactggc aaccaccggt ttgcatctgg ggagtggcag cgataatgga tttttccata
      841 cgctggcggt gccgctgttt atctttacca attttattat tgcaatactg ctcatccgta
      901 cttttgcgct tctgatgcag ggaaaattgt tagtcagaac cgagcgcgcc gttttaatga
      961 aagcagagga caaagaatga tcattcgtga cgaaaattat tttactgata aatatgaatt
     1021 aacccgcaca cactctgaag tactggaagc ggtgaaagtg gttaaaccgg gtaaaacgct
     1081 ggatctgggc tgtggcaatg gtcgtaacag tctttacctg gcagccaatg gttatgatgt
     1141 tgacgcatgg gataaaaatg ccatgagtat cgccaacgtc gagcgcatta aatctattga
     1201 aaatctggat aatttacaca ctcgggtcgt tgatctgaat aacctcacat ttgctggaca
     1261 gtacgatttt attctttcga ctgtggtgct gatgttcctt gaggctaaaa ccatcccagg
     1321 gctgattgcc aatatgcaac gttgcacgaa acctggcagt tacaacctga ttgtggcagc
     1381 gatggatacc gctgattatc catgtaccgt cggcttcccg tttgccttca aagagagaga
     1441 attacgtcga tattacgaag gctgggagat ggtgaaatac aatgaagacg tcggcgagct
     1501 gcaccgcacc gacgccaacg gtaatcgtat taaactgcgt ttcgccacga tgctggcacg
     1561 taaaaaatga cccggtaaga acaaagcgcg tgaaaattcc ccacgctgag atggtttact
     1621 gttcttcttt tcggtaagca tattttttat cgaagggatg tgaaattaat cacagtagtc
     1681 gaagtttttt agcagcttaa cttactgaaa tttaactact gatgattgac ttagcccctt
     1741 tttcggcatt gactatgtcg tctgaaaagg ggctgaaaaa tttattttca ccaacacttt
     1801 ttttgccaca acacgaagcg gcgctttttg ctataactta aaaagtaata taatcatctc
     1861 aggaaactat tcatgcgtac cacatcattt gcaaaagttg cagctttatg cggcttgttg
     1921 gctctgtctg gttgtgcctc taaaatcacc cagccagata aatattctgg ttttttaaac
     1981 aattactctg atttaaaaga aacaacctcg gctacaggta aaccggtttt acgttgggta
     2041 gacccgagtt ttgatcaaag caaatatgac agcatcgtct ggaacccaat cacttattat
     2101 ccggtaccga aaccttcaac ccaggtaggg cagaaagttc tggataaaat tttgaactat
     2161 accaacaccg aaatgaaaga agcgatagcg cagcgtaaac cactggttac caccgctggg
     2221 ccgcgtagtc tgattttccg tggggccatt accggtgtag ataccagcaa agaagggctg
     2281 caattctatg aagtggttcc tgttgcatta gtggttgcgg ggacgcaaat ggctacaggc
     2341 caccgtacca tggatactcg cctctatttt gaaggtgagc tgattgatgc agcgactaat
     2401 aaaccggtta tcaaagtcgt tcgtcagggc gaaggtaaag acctgaataa cgaaagtacg
     2461 ccaatggctt tcgaaaatat taaacaagtt attgatgaca tggcgaccga tgccaccatg
     2521 tttgacgtta acaaaaagta gttccagacg cgccatcgtt agatggcgct ttttatccgg
     2581 tgcgccgtaa aaccccatcc ttcagggcgg ggatataagg cgcggttttc cacctgacca
     2641 ggtgtttgct gttgttcaat gtattggcgg atgacggata ttggcgcacc tccgcagcta
     2701 ctggcaaaat agccaggact ccacaaaacg cctttgtagt aatacctgac tgcaatatct
     2761 ggtcgatctt gtcgcagtaa cctacccgat acgcctttga ggctgtttac cagactggat
     2821 atcgccagtt tgggaggtta gttaattaac aaatggacgt gatctggttc gccatccatt
     2881 tcaaccagtt cagcttcaaa ataagcacat acatttgaaa agtaagtgcg tagtttttct
     2941 gtcgcgtcat ggtcaaaaat ctggcgtcgg tatctggtga caaagaccag gtgaacatgc
     3001 atcaggaaaa cacaatgcct tccacgccgg atatcggttt cttttttcac agaccaaagt
     3061 atcatttgac ctgtgaaacg attacaggca tttaaattcc agttaagacc aggtggtcaa
     3121 caggagtgtg aaatgaggcg cttcgcaggc gcatgtcgtt tcgttttcaa tcgtgctctg
     3181 gcacgtcaga atgagaatca tgaggccggt aataaataca tcccttacgg gaaaatggct
     3241 tcctggctgg ttgagtggaa aaatgccact gaaacgcaat ggcttaaaga ttctccctca
     3301 tagccattgc aacagtcact gaaagacctt gagcgggctt acaaaaactt cttccggaag
     3361 cgggctgctt ttccccgatt caaaaagcgg ggacagaatg atgcattccg ctacccgcag
     3421 ggcgttaagc tcgatcagga aaacagccgt atttttctgc cgaaactggg ctggatgcgc
     3481 taccggaaca gccgtcaggt cacgggtgtt gtgaaaaatg tcactgtcat ccagtcctgc
     3541 ggtaagtggt acatcagtat tcagacagaa agtgaagtat ccactccggc tcacccttca
     3601 gcatcaatgg tcgggctgga tgctggcgtg gctaaactcg ccacgctgtc agatggcaca
     3661 gtctttgagc ctgtaaacag ttttcagaaa aaccagaaga cgctggcgag acttcagcga
     3721 cagttaagcc gcaaggtcaa attcagcaac aactggcaga agcagaaacg caaaatacag
     3781 cgactgcatt cctgtatcgc aaatatccgc agggactacc ttcacaaagt cacaacggcc
     3841 gtcagcaaaa accacgcaat gatagtcatt gaggatttga aggtcagcaa catgtcaaag
     3901 tcagcagcgg gtacggtcag ccagccgggg cgcaatgtcc gggcaaaatc aggtttaaac
     3961 cgttcgatac tggatcaggg ctggtatgaa atgcgccgcc agcttgagta caagcagctc
     4021 tggcgtggcg gtcaggtgct tgctgttccg ccagcgtata caagccagcg ttgcgcgtac
     4081 tgtggtcata cagcgaaaga gaaccgcctg tcacaaagta aattcagatg ccaggtatgt
     4141 ggatatacag cgaacgccga tgtaaatggc gctcgtaaca ttttagcggc ggggcacgcc
     4201 gttcttgcct gtggagagat ggtgcagtca ggccgcccgt tgaagcagga acccaccgaa
     4261 atgattcagg cgacagcctg aacgtagcag ggatccacgt ccttcagggc gtggaggatg
     4321 tcaatatcgg tttctgtcag cgattaaatt caacaccacg taacaaacgc ctccggcaat
     4381 taatccccag aacgcagaac caatcccgac cagcgtcaat ccacttgccg ttaccagaaa
     4441 tgccaccacc gccgcgtctc gctcacgctc attatgcagc gcctgataca aactgccgct
     4501 aatggtactt aacagcgcca gacctgccag catctggatc caacttacgg gcagggcagc
     4561 catcatcccg gtaatagcac taccaaacag acctgcgatc aaataaaaaa tgcctgcaac
     4621 ggcagctgcc agccaacgtt gatctttatc cggatgcgct tccgggcttt ggcaaatagc
     4681 cgcggtgatt gccgcaatac cgacggaata aacgccgaaa ggggaaaaaa ccagtgccag
     4741 caatccagta aatacaatta atggcgaaac aggagccgaa tacccggctg ctttcattgc
     4801 tgcgataccc ggtgcgtttt gcgatgccat cgtcaccaga aaaaggggga gtgcaacgct
     4861 caggctgtga gcaaacgaaa aatcaggggt aatataagtg gggagaacgg gtttaaagac
     4921 aacatcagtt gtgacaacgt caccttgcgc gataacgatc acgatcccaa taatcatcgc
     4981 ggcaattacc gcatagcgcg gcgcaacggc cttggttgcc agccatacca gcaacatact
     5041 tccacacaac gtaaattgac cgtccagact ggcaaacgcc tgtaaaccaa agcgtaataa
     5101 aatcccggca agcattgccg ccgcaagcga gtgcggaata atgcgcatca gacgagcaaa
     5161 gagtcccgtt atgccgcaga ggactattaa cgcgttggtg acaataaaaa cgccgatggc
     5221 ttcgttaagt gttagtccct gcaatccggt gaccaacaaa gccgcgccag gcgttgacca
     5281 tgcggtgaga acaggtacgc gataccataa tgtcagagtc agcgtactga cgcccattgc
     5341 cagccccagc gccgtcatcc agccagagat ttgtgcagtg gaggctccgg cgacaatcgc
     5401 tgcttgccag attattgccg ctgaactggc gtagccaatt aataccgcca gaaaccccgc
     5461 cagtagcgtg ggtggaggaa tagaaaacag acgcatagtc acctcgtgcg ttatagcgta
     5521 caggcaccgt agcatttgtc cgttataacg cacaagtgat aaacttccgt tttgccggag
     5581 gagtcgcatg gaaaatctcg ctcgcttttt atccaccaca cttaaacaat tacgtcagca
     5641 gcgcggctgg agtctttcgc gactggcaga agcgacgggc gtttctaaag caatgttagg
     5701 acagattgag cgtaatgagt ccagcccgac ggtcgcgaca ttatggaaaa ttgccactgg
     5761 gttgaacgtg cccttttcga catttatttc tccgccacag tccgctacgc cttcagttta
     5821 tgatccacaa cagcaggcga tggtgatcac ttcgctgttt ccttatgatc cacaactctg
     5881 ctttgaacac ttctcgattc agatggcacc cggtgcaata agcgaatcga cgccccatga
     5941 gaagggggta atcgaacatg tcgtcgtcat tgatggacaa cttgatctgt gcgttgatgg
     6001 ggaatggcag agccttaatt gtggggaagg cgttcgattt gctgcagacg tcacgcatgt
     6061 ctatcgtaat ggtggggagc aaaccgtaca ttttcattcc ctcatccatt acccgcgcag
     6121 ttaagcggga aaactatttc gcaacgtcgc gcttgtggct aaaatagccg ccatttttca
     6181 gctactggat aagaatgtga ccgtatcttc tcatcgactt gaactgttaa gcccggcacg
     6241 cgatgccgcc attgcccgcg aagctatttt gcacggtgct gatgctgttt atatcggcgg
     6301 ccctggtttt ggtgcccgtc ataatgccag taatagcctg aaagatattg ccgagctggt
     6361 gccgtttgcc catcgttatg gtgcaaaaat tttcgtcacg cttaacacca ttttgcatga
     6421 tgatgagctg gaacccgcgc aacggctgat tactgacctc taccagaccg gtgtcgatgc
     6481 gctgattgtt caggatatgg ggattctgga acttgatatt ccgccgattg aactgcacgc
     6541 cagtacgcag tgcgacattc gtacagtaga aaaagcgaag ttcctctctg atgttggctt
     6601 cacgcagatt gtgctggcgc gagagctgaa tcttgatcag atccgcgcga ttcaccaggc
     6661 cacggacgcg accattgaat tctttattca tggcgcactg tgcgtggcct attcgggtca
     6721 gtgctacatt tctcatgcgc aaacagggcg tagcgccaac cgtggcgatt gctcgcaggc
     6781 gtgccgtttg ccatacacat tgaaagacga tcaggggcgg gtggtttcct atgaaaaaca
     6841 tctgctgtcg atgaaagata acgatcagac tgccaacctc ggcgcgctga ttgatgctgg
     6901 cgtacgctcc ttcaagattg aagggcgtta caaagatatg agctacgtga agaatatcac
     6961 cgcccattat cgccagatgc tggatgccat tattgaagaa cgtggcgatc tgacgcgcgc
     7021 ttcatcaggt cgtactgaac atttctttgt tccatcgacg gaaaagactt tccaccgtgg
     7081 tagcacagat tattttgtga atgcccgtaa aggcgatatt ggcgcgttcg attcgccgaa
     7141 atttatcggc ctgccggtgg gtgaagtatt gaaagtggcg aaagatcatc ttgatgttgc
     7201 cgttaccgat ccactggcaa atggcgatgg cctgaacgtg ttgattaaac gtgaagtcgt
     7261 cggttttcgt gccaatacgg tcgagaaaac cggagaaaat cagtaccgcg tctggcccaa
     7321 tgaaatgcca gcagatttgc acaaaattcg cccgcatcac ccactaaacc gtaatcttga
     7381 tcataactgg cagcaggcac tgacaaaaac ctccagcgaa cgtcgggtgg cggtagacat
     7441 tgaactgggc ggttggctgg aacaattgat tctgaccctc accagtgaag agggggtcag
     7501 catcacgcat acgctggatg ggcagttcga cgaagccaat aacgccgaaa aagcaatgaa
     7561 caatctgaag gatggtctgg caaaactggg gcaaaccatc tattacgccc gcgatgtgca
     7621 aattaatttg ccgggggcgc tgtttgtacc aaacagtctg ttaaaccagt tccgccgtga
     7681 agctgctgac atgctggatg ctgcgcgtct tgccagttac cagcgtggca gccgtaaacc
     7741 ggttgctgat cctgcgccgg tttatccgca aacgcatctg agtttcctcg cgaacgttta
     7801 caaccagaaa gcgcgtgaat tttatcatcg ctatggtgtg cagctgattg acgcggcgta
     7861 tgaggcacat gaagagaagg gcgaagtccc ggtgatgatc accaagcatt gtctgcgctt
     7921 tgcctttaat ttgtgcccga aacaggcgaa aggcaatatc aaaagctgga aggctacgcc
     7981 aatgcaactg gttaacggcg atgaagtatt aacgctaaag tttgattgcc gcccatgcga
     8041 gatgcacgtc attggcaaaa tcaaaaatca catactgaaa atgccgttac cgggaagcgt
     8101 agtggcatcc gtaagtccgg atgagctgct gaaaacattg ccgaagcgaa aagggtaaaa
     8161 cgccagtttt ctggttactc acaacttatt gaatctgcat gatattgcct gccgggtaag
     8221 gcgttacgcc gcatccggca tcaaacgact caacgttgtc ccgcttctgg tttgcgcgat
     8281 ttttgccagt aaaaatgctc gcgcaaacct tccgccgact cttccgccac agcacgcaat
     8341 tcatcgctgt ccgcttcatg acgcagctga tgatccacat tctttaccca cacaaattca
     8401 tgtcctttgt gccctgccat gagttgtcct gagaacagcg cacacgttaa taagacaacc
     8461 gataacgcct tcgtaaacat cctgccacct ttttcttacc ttttgccgct atgatgccga
     8521 tcgtttcttg aggttattat tcagttttgc aaattagcgc aaagaaattc tggaatcttc
     8581 cttcctgatt ttgcattgca ttctgccgtt gcggcgattt agtgctattt tcgagcaaat
     8641 tacacacgga ggtaaacact aatgtttact ttgttgatat actcagcggc agggaggcga
     8701 tgtgaaacaa agcgagttca gacgttggct cgaatctcag ggcgtcgatg tagcgaatgg
     8761 cagcaaccat ttgaaactca ggtttcatgg gaggcgcagt gtcatgccgc gtcacccctg
     8821 cgatgagatt aaagaaccat tgcgtaaagc aatcctgaaa caactcggtt tgagttaatc
     8881 gccaattaaa aaggttaatg acatgcgaga gacagtcgaa attatgcgtt atcccgtcac
     8941 tcttacaccc gcgccggaag gcggttatat ggtttctttt gtggatatcc ctgaagcgtt
     9001 gacgcagggc gaaactgtcg ctgaagcgat ggaagcggca aaagatgctt tacagaccgc
     9061 atttgatttt tattttgaag ataacgagct tatcccttta ccttcgccat taaatagtca
     9121 tgatcacttt attgaagtac ctttgagcgt cgcctctaag gtattgctgt taaatgcttt
     9181 tttacagtca gaaatcactc agcaagagtt agccaggcga attggcaaac ctaaacagga
     9241 gattactcgc ctatttaact tgcatcatgc gacaaaaatc gacgccgtcc agctcgcggc
     9301 aaaggcgctt ggcaaagagt tatcgctggt gatggtttaa ttacagttaa cgaaaagttg
     9361 tcatttttaa caactgatat agactgccga atcatctgca cataattacg attcgataat
     9421 gaaaaaatac cagcagcttg cagaacaatt acgcgagcag attgcgtcgg gtatctggca
     9481 acccggcgat cgtttgcctt cgttgcgtga ccaggtggcg ctttctggca tgagctttat
     9541 gactgtcagc catgcctatc agttgctcga aagtcaggga tatattatcg cacgaccgca
     9601 gtcgggttat tacgttgcgc cacaggcaat aaaaatgccg aaagtgccag tcattccagt
     9661 cactcgagat gaagcagtcg atatcaacac ttatattttt gatatgttgc aggccagtcg
     9721 cgatccgtcg gtcgttccgt ttgcctcggc ctttcccgac ccgcgacttt tccccctcca
     9781 acaactaaac cgctcgctgg cgcaggtaag caaaaccgcc acagcgatga gtgtgattga
     9841 aaacttaccg ccaggaaacg cagaactgcg tcaggctatt gctcgtcgct atgccttaca
     9901 gggcatcacc atttctcctg atgaaattgt catcactgcc ggggcgttag aagcattaaa
     9961 cctcagtttg caagcggtaa ctgaaccggg cgattgggtg atagtagaga atccttgttt
    10021 ctatggcgcg ttgcaggcgc tggaacggct acggctgaaa gcgttatcgg tagcgacgga
    10081 tgttaaagaa gggatcgatc ttcaggcgct ggaactggcg ttgcaggagt atccggtgaa
    10141 agcgtgctgg ctgatgacta atagccagaa tccactcgga tttaccttaa cgccgcaaaa
    10201 aaaagcacaa ctggtggcgt tgctcaatca gtacaacgta acgctgattg aagatgacgt
    10261 ttacagcgaa ctttattttg gacgggaaaa accgctgcct gcgaaagcgt gggatcgcca
    10321 cgatggcgtt ttgcattgct cttcgttttc gaaatgtctg gtgcctggtt ttcgtattgg
    10381 ttgggtcgcc gccggaaaac atgcacgtaa aattcaacgc ttgcagctga tgagtacgct
    10441 ttccaccagc tcaccgatgc agcttgcgct ggtggattac ctttccacgc gccgatacga
    10501 cgcccatctt cgtcgcctgc gtcgccagct tgcggaacgt aaacaacgtg cctggcaggc
    10561 actgctgcgt tatctgcctg cggaagtgaa aattcatcat aatgacagcg gttattttct
    10621 ctggctggag ctccccgagc cgttagatgc cggcgaattg agcctggcgg cactgacgca
    10681 tcatatcagt attgcgccag gtaaaatgtt ttctaccggt gaaaactggt cacgtttttt
    10741 tcgttttaat accgcgtggc agtggggaga acgtgaagaa caggcggtaa aacaattagg
    10801 caaacttatt caagaacggc tgtaatagcg tttaatttaa ttcctcttag attgggtaat
    10861 atgaatttcg aatagcagtc atatttccta actccttgtc tatactccag aagataacct
    10921 tacagacggc ataatgcgcg gtagctcaca acctgattaa attttctcag gggcgaaggt
    10981 gtgcctgcaa gccgccgtct atggttaaac aaggagatat ttttacggca cggcggctga
    11041 acaattaatt acgacaggag taagacctta tgagcaagac atttgcccgc agcagcctgt
    11101 gtgcgctcag catgacaata atgaccgctc acgccgccga accgcctacc aatttagata
    11161 aaccggaagg gcgactggat attatcgcct ggccgggata catcgaacgc ggacaaaccg
    11221 ataaacaata cgactgggtg acgcaatttg aaaaagaaac aggttgcgcg gtgaatgtga
    11281 aaactgcggc gacttccgat gaaatggtca gtctgatgac caaagggggt tacgatctgg
    11341 ttacggcatc cggcgatgcc tcgctgcgtt tgattatggg taaacgcgtg cagccgatta
    11401 ataccgcatt gattcccaac tggaaaacgc tcgatccgcg cgtggttaaa ggcgactggt
    11461 ttaacgtggg cggcaaagtt tacggcacac cttaccaatg ggggccgaac ctgctgatgt
    11521 acaacactaa aaccttcccg acgccgccgg atagctggca agtggttttt gttgagcaga
    11581 atctgccgga cggcaagagc aataaaggcc gcgttcaggc ttatgatggc cctatctaca
    11641 ttgcggacgc tgcgttgttc gttaaagcca ctcagccgca gttgggcatc agcgatccgt
    11701 atcaactcac cgaagaacag taccaggcgg tgctgaaagt gctgcgcgct caacatagtt
    11761 tgatccatcg ctactggcat gacactaccg tgcaaatgag cgatttcaaa aacgagggtg
    11821 tggttgcttc cagtgcatgg ccctatcagg ccaacgcctt gaaagccgaa ggccagcctg
    11881 ttgctaccgt tttcccgaag gagggtgtta ccggttgggc tgataccacc atgctgcata
    11941 gcgaagcgaa acatccggtt tgcgcctaca aatggatgaa ctggtcatta acgccaaaag
    12001 tgcagggcga tgtggcggcc tggtttggct cgttaccggt agtgccggaa gggtgtaaag
    12061 ccagtccgtt attaggcgaa aaaggttgtg aaaccaacgg ttttaactat ttcgacaaaa
    12121 tcgccttctg gaaaacgcct atagcagaag ggggcaagtt tgttccctac agtcgttgga
    12181 cgcaggatta cattgccatt atgggcggtc gctaacttcg ctggggtgct ttatgacgta
    12241 cgcagtggag tttgacaacg tctcgcggtt gtacggtgac gtgcgcgcag tagatggcgt
    12301 cagtattgcg ataaaagatg gtgagttctt ctctatgctg gggccgtccg gctccggcaa
    12361 aaccacctgc ctgcgcctga ttgctggctt cgaacagctt tccggcgggg ctatctctat
    12421 ctttggtaaa cccgccagca atctgccacc gtgggagcgg gacgtgaata ctgtctttca
    12481 ggactacgcg ctatttccgc atatgtcgat tcttgacaac gtcgcctatg ggctgatggt
    12541 caaaggcgtg aataaaaagc agcggcacgc aatggcgcaa gaggcgctgg agaaagtggc
    12601 gttggggttt gtacagcaac gtaaaccgtc acaactttct ggtggtcagc gccagcgggt
    12661 tgctatcgcc agagcattgg tgaatgaacc gcgcgtattg ctgttggatg aaccgctcgg
    12721 cgcactggat ctcaaattgc gtgagcagat gcagctggaa ctgaaaaaac tgcaacagtc
    12781 tctcggtatc acttttatct tcgttaccca cgatcagggc gaagcgttat cgatgtccga
    12841 tcgtgtggcg gttttcaata atggacgcat tgagcaggtc gattccccgc gcgatctcta
    12901 tatgcgcccg cgcacgccgt ttgttgccgg attcgttggg acatcgaatg tttttgatgg
    12961 actgatggca gagaaacttt gtggcatgac gggaagcttc gcactacgac cggaacatat
    13021 ccgtctcaac accccaggtg aactgcaggc caatggcacg atccaggcgg tgcaatatca
    13081 gggcgcggca actcgttttg aactgaaatt gaacggcggt gaaaaactgc tggtgagtca
    13141 ggccaatatg acaggcgaag aactgcctgc cacgctcacg cccggacaac aggtgatggt
    13201 ttcctggtcg cgtgatgtga tggtgccgct ggttgaggag aggtgaatgg cgatgaacgt
    13261 attgcaatca ccttcacgtc caggtctggg taaggtgtcc ggtttcttct ggcggaatcc
    13321 ggggctgggg ctgtttttgc tgctgcttgg cccgctaatg tggtttggca ttgtctattt
    13381 cggctcgctg ctaacgctgt tatggcaggg cttttatacc tttgacgatt tcaccatgtc
    13441 ggtaacgccg gaactgacgc tggcgaacat ccgtgcgctg tttaatccgg caaattacga
    13501 catcattctc cgcacgctga ctatggctgt ggcagtcact atcgccagcg ccattctggc
    13561 ttttccaatg gcgtggtata tggcgcgcta taccagcgga aaaatgaaag cgttttttta
    13621 tatcgcggta atgttgccga tgtgggcgag ctacattgtt aaagcctatg cctggacgtt
    13681 attgctggca aaagatggcg tggctcagtg gtttttacaa catctggggc tggaaccact
    13741 gctgactgcg ttccttacat tacctgcggt gggcggcaat acgctgtcaa cttccgggct
    13801 ggggcgcttt ctggtgtttc tctatatctg gctgccgttc atgatcctgc ccgtgcaggc
    13861 ggcgcttgag cgtttgccgc cgtcattgtt gcaggcgtca gctgatctcg gcgcacgtcc
    13921 acgacaaacc tttcgctatg tggtgctgcc gctggcgatc ccgggtattg ccgctggctc
    13981 tatctttacc ttctcactca cactgggtga ttttatcgtc ccgcaactgg ttggccctcc
    14041 aggatatttt atcggcaata tggtttattc ccagcagggg gcgattggca atatgccgat
    14101 ggcggcggca ttcaccctgg tgccgattgt tctcatcgca ctgtacctgg cgttcgtgaa
    14161 acgtctggga gcgttcgatg cactctgaac gcgcaccgtt tttcctcaaa ctggcggcct
    14221 ggggcggcgt agttttccta cattttccta tcctgattat cgccgcctat gcgtttaaca
    14281 ctgaagatgc ggcgtttagt tttccaccgc aaggcctgac gctgcgctgg tttagcgtgg
    14341 cagcacagcg tagtgatatt cttgatgccg tgacactgtc acttaaagtg gcggcgctgg
    14401 cgacattaat cgcgctggtg ttagggacgc ttgcagctgc cgcgctgtgg cgacgagact
    14461 ttttcggcaa aaacgccatt tcgctgttac tgctgctgcc cattgcgctg ccgggcattg
    14521 tcactggtct ggcgctatta accgctttta aaaccatcaa tctggagccg ggatttttca
    14581 ccatcgtggt cggccatgcg actttttgcg tagtggtggt gtttaacaat gtcatcgccc
    14641 gttttcgccg cacctcgtgg agtctggttg aggcgtcaat ggatcttggg gccaatggct
    14701 ggcaaacctt ccgctacgta gtgttgccga atctcagttc ggcgttactg gcaggaggaa
    14761 tgctggcgtt tgccttgtcg ttcgatgaaa tcatcgttac gacctttacg gcaggtcatg
    14821 aacgaacgtt accgttgtgg ttgctcaatc agcttgggcg accgcgtgat gtaccggtaa
    14881 ctaacgtggt ggcactgctg gttatgttgg taacaacctt gccgatcctg ggggcctggt
    14941 ggctaacccg cgaaggcgac aatggtcaat aaccactgat acaggaatat gctatgcaac
    15001 ataagttact gattaacgga gaactggtta gcggcgaagg ggaaaaacag cctgtctata
    15061 atccggcaac gggggacgtt ttactggaga ttgccgaggc atccgcagag caggtcgatg
    15121 ctgctgtgcg cgcggcagat gcagcatttg ccgaatgggg gcaaaccacg ccgaaagtgc
    15181 gtgcggaatg tctgctgaaa ctggctgatg ttatcgaaga aaatggtcag gtttttgccg
    15241 aactggagtc ccgtaattgt ggcaaaccgc tgcatagtgc gttcaatgat gaaatcccgg
    15301 cgattgtcga tgtttttcgc tttttcgcgg gtgcggcgcg ctgtctgaat ggtctggcgg
    15361 caggtgaata tcttgaaggt catacttcga tgatccgtcg cgatccgttg ggggtcgtgg
    15421 cttctatcgc accgtggaat tatccgctga tgatggcagc gtggaaactg gctccggcgc
    15481 tggcggcagg gaactgcgta gtgcttaaac catcagaaat taccccgctg accgcgttga
    15541 agttggcaga gctggcgaaa gatatcttcc cggcaggcgt gattaacgta ctgtttggca
    15601 gaggcaaaac ggtgggcgat ccgctgaccg gacatcctaa agtgcggatg gtgtcgctga
    15661 cgggctctat cgccaccggc gagcacatca tcagccatac cgcgccgtcc attaagcgta
    15721 ctcatatgga acttggtggc aaagcgccag tgattgtttt tgatgatgcg gatattgaag
    15781 cagtggtcga aggtgtacgt acatttggct attacaatgc tggacaggat tgtactgcgg
    15841 cttgtcggat ctacgcgcaa aaaggcattt acgatacgct ggtggaaaaa ctgggtgctg
    15901 cggtggcaac gttaaaatct ggtgcgccgg atgacgagtc tacggagctt ggacctttaa
    15961 gctcgctggc gcatctcgaa cgcgtcagca aggcagtaga agaggcgaaa gcgacagggc
    16021 acatcaaagt gatcactggc ggtgaaaagc gcaagggtaa tggctattac tatgcgccga
    16081 cgctgctggc tggcgcatta caggacgatg ccatcgtgca aaaagaggta tttggtccag
    16141 tagtgagtgt tacgctcttc gacaacgaag aacaggtggt gaactgggcg aatgacagcc
    16201 agtacggact tgcatcttcg gtatggacga aagatgtggg cagggcgcat cgcgtcagtg
    16261 cacggctgca atatggttgt acctgggtca atacccattt catgctggta agtgaaatgc
    16321 cgcacggtgg gcagaaactt tctggttacg gcaaggatat gtcactttat gggctggagg
    16381 attacaccgt cgtccgccac gtcatggtta aacattaagg ataatattgc agatcgtaag
    16441 agtatgagat gatcttacgt actgtcagag cactgatttc tctgacagta cgtgctgcgt
    16501 gcaaaatcac tacatcaaat aaaccagcca aatctcatag agataagagt aaacaaacca
    16561 acagcagcaa agacatttat cagtactaca gttctactgg aaacattcat ttttgccacc
    16621 ttttatcatt gcccctatgt gtatagtacg ggttaagaaa atccgcaata atactcattc
    16681 tgaagatgga taagggcaag ttgctgtttg atgttttttt ctcaaaccgg ttaaaaaact
    16741 tgatgcacgt caaaaaatga cgcatatttg cgcgcgtttt attcatctgg ctggacgccc
    16801 gtacatgtct ctctatcaac atatgcttgt tttttatgcg gttatggcag caatctcatt
    16861 tcttatcacc tggtttcttt ctcacgataa gaaacgcatc cgtttcttaa gcgcctttct
    16921 ggtgggggca acatggccga tgagtttccc ggtggcgctg ttgttttcac tgttttaaat
    16981 cttactcact ggcttgacgg caccacgcag acttatatca tttggatgaa tcgataaatt
    17041 tcacaagtgg ctaaggagaa agtatgtcgc atctggatga agtcatcgcg cgcgtggatg
    17101 ccgcgattga agagagcgtc attgcccata tgaacgaatt attaatcgcc ctgagcgatg
    17161 acgcggagtt aagtcgggaa gatcgctaca cccagcagca acgtctgcgc acagcgatcg
    17221 cccatcacgg tcgcaagcat aaagaagata tggaagcgcg ccacgaacag ttaaccaaag
    17281 gcggcaccat cctgtgatta aaatgagcgt ctggccacca accaggcgcc aaccacaagt
    17341 aaaattgcgc cacaaattgg gccgaacaac gcccgtagtg gtacgccatg actgcggaag
    17401 atatccagca ccagaccgcc aatcagctgg ctggcgacca gtactgcaat agttgtcgcc
    17461 gctcccacat tctgatatcc gctgatactg gcgaagacaa aaaacgatcc cagcaagcca
    17521 ggtataagtg tccaccagcg cacgctggac accagttcgc caaaccccgc cataccttgt
    17581 ttaaaccata aaatagaaac aaagagcaca atccccacca gtgagttcag caacatggcg
    17641 atgagaatgg tagaggaggt ctgggtgatg cgcaccatca gcgtgttctg gactaccaga
    17701 ccaattccgg cggcaatcaa aaaggcaagg gtgagcgact gattcatcca atcgcgtccg
    17761 gttcagtgcg ttcgtcgagt tgcaactgca taaatgtcag atccagccag cgcccaaatt
    17821 tagtgcccac ctgcggcatt tgtgcggtga cgacaaatcc cagcgactgg tgcagatgca
    17881 gcgaggcctg attttgcgat tcgatcccgg cgaccatgac atgcttcccg caatctcgcg
    17941 cttcatcaat caatcggctt aacaatttac gccccagacc tttgccctga tgatcgggat
    18001 ggacataaac cgaatgttcc acggtatggc gaaaaccatc gaaactacgc cagtcgccaa
    18061 atgaggcata tcccgtcact acgccatctt cttcacttac cagcaccgga taacctgcta
    18121 tggtccgcgc ttcaaaccag gcaatgcggt tatctgcatc caccgtttgg tcattccaga
    18181 tagccgccgt atacaacacg gcgtggttat aaatttccgc aatggcagca cagtcggctt
    18241 tgcgggcaaa acggatggac atgtctggcc tcaataaaat aatgatgatg atgttatcaa
    18301 tactatcgaa aacagcccga agcaacggat tcctcgatga aattattgcg tgttgcagag
    18361 ggggtgtgac ggctgcaaac aaaaccaatc ctgcgagcct gctactattg atggattcat
    18421 gccaaaagca caggagagca ttatggggca acaaaagcag cgtaatcgac gttgggttct
    18481 ggcctcgcgt ccacatggcg caccggttcc ggagaatttc cgtcttgaag aagatgatgt
    18541 cgccacaccg ggtgaaggac aggtgttact gcgcacagtt tatttgtccc tggacccgta
    18601 tatgcgtgga cgtatgagcg atgagccatc ttattcaccg ccggttgata ttggcggcgt
    18661 gatggtcggc ggtacggtga gccgtgtcgt ggagtcgaat catcctgatt atcagtctgg
    18721 cgactgggtg ctgggctaca gtggatggca agactatgac atatccagtg gtgatgatct
    18781 ggtgaaactt ggcgatcatc cgcaaaatcc atcgtggtcg ctgggtgtgc tagggatgcc
    18841 aggctttacc gcttatatgg gcctactgga tatcggtcag cctaaagagg gcgaaacgtt
    18901 ggtggtagct gcggcgacag gaccagtggg ggcgacggtg gggcaaatcg gcaaacttaa
    18961 aggttgcaga gtggtggggg tagccggtgg cgcggaaaaa tgccgccatg ctaccgaggt
    19021 gttaggcttc gatgtttgcc ttgatcacca cgcggacgat tttgccgaac aactggcgaa
    19081 agcgtgccca aaaggtattg atatctatta tgaaaacgtg ggcggtaagg tatttgatgc
    19141 tgtgctacca ttacttaata catctgcgcg cattcccgtt tgtggcttag tgagcagcta
    19201 taacgctaca gagctaccac ccggtccgga tcgtttaccc ctgttgatgg caacggtgct
    19261 gaaaaaacgt attcgcttgc aagggtttat tatcgctcag gattatggtc accgcatcca
    19321 tgagtttcag aaggagatgg ggcaatgggt gaaagaggat aaaatccact accgcgaaga
    19381 aattactgac ggtttagaga atgcgccaca gacgtttatc ggcctgctga agggtaaaaa
    19441 cttcggcaaa gtggtgatcc gcgtggcggg tgatgattaa agtttcaacg gcggcgtaag
    19501 ccgccataaa aatccaggca aaaagcttat tttcagcttt aattaactaa ccacatcatt
    19561 gttctgctgc taataacaac aaaggtgagt ggtttatgat tattcacttt aatacaccag
    19621 gtgaattcct tctgccatgc aggcagggtt ggacagaaaa cacgcgaagg aattaaaatg
    19681 cctggaacgg aaaaaatgaa acatgtcagt ttgactctgc aggttgagaa cgacctgaaa
    19741 catcagctta gtattggcgc actaaaacct ggcgcacgcc tgattactaa aaatctggcg
    19801 gagcaattag gtatgagtat tacacctgtg cgtgaagcat tattacgtct ggtttcggtg
    19861 aatgcgcttt ctgtcgcacc agcgcaagca tttacagttc cggaagtggg gaaacgtcaa
    19921 ttagacgaaa tcaatcggat ccgctacgaa ctggaattaa tggcagttgc tctggctgtt
    19981 gaaaacctca ccccgcaaga cctggcggaa ctccaggaat tgctcgagaa attacagcag
    20041 gcgcaagaaa agggcgacat ggaacaaatc attaatgtaa acaggctatt tcgcttagcg
    20101 atttatcatc gctcaaatat gcccatcctg tgtgagatga ttgagcaact gtgggtcagg
    20161 atgggacctg gtttacatta tctctatgaa gcgattaatc cagcggaatt acgggagcat
    20221 atagaaaact atcatctgtt actcgctgcg ttaaaagcaa aagacaaaga gggatgcaga
    20281 cattgtcttg ctgaaattat gcaacaaaat attgctattt tataccagca atacaatcgt
    20341 taaagtaaat gcccggcaaa taccgggcat caaggtaatt actcaaatcg ccatgcgata
    20401 ttcacgccga caccataatt tcgtccgggc gcaggttcgt aatatcgccc gtttgactca
    20461 ttgacaatga cagaaccaac gtattcttta tcgaataaat tatcgacacg accaaataaa
    20521 tccacagtta aattgtggta attgtattta tacccggtga ataagccgac gagagtataa
    20581 gacggcgctt tggccgtatt ttcatcatct gccataatat cgcccatata acgcgcttcc
    20641 gtgcctgcat accagccatc ttccggtaca taacctatcg acgcaaagcc catattacgg
    20701 gcgatccctg gcatccgatt accgttacaa tcctgttcat tgcaaacatt gctgcgatag
    20761 gtcgcatcaa gccaggtcca ggacgcgttt acgcgaaaat ctcccgcgaa acgttgatcc
    20821 catgccagtt cagcgccttg acgacgggtc tttccagcat ttttgtaagt cgtacgccca
    20881 ccgctactgc tatcgacaac aatttcatca tcggtgtcgg tctggaacaa tgcgagacta
    20941 agcagcccat caccaatacg cgttttactg ccgatctcaa ttgtatcgtt ggtggatggt
    21001 tttaaaccaa agttcatacc gctttgccca tcagcgcgat aagacagctc attaatcgtc
    21061 ggcgtttcaa aaccacgccc ggctgccaga tagatattcc aggcatcggt cattgcatat
    21121 tttaacgatc cggctggtag ccatttgtga taactggcat caccgctgtc atcgccgtta
    21181 cccggagtaa tgtaatggtc gttggaatca aaccacacgg agctgtagcg cacgccagca
    21241 tccagcgaca gtttttcgct cagctgccac tgcgtctgta aatagggatc gatgttccac
    21301 atcagattgc gttcgtcgcg acgcaactca cctttttgcc cgtactccgg catgccgcta
    21361 ttcaggcgga agttattgta gcccttgcgg ttttcactca tgttttcgta gttcaggccg
    21421 gtagtgaacg tgaccggaac gcccagttca ccacggtgtg tccagcggct gtctattccc
    21481 tggtaatggc gttgcagggt aatcacgccg cccgcatgtg acgggttaag ttgtggtgcc
    21541 atgggtattg actggtactg ggtcgtttct cgctctccgg catacatcat cacactcata
    21601 tcatcccgcg aactcaggct acgttcatag cgcaacccag cctgagtttg cttgatggtt
    21661 tttcgcgtgt cgtactgttc tgcacgaggc gcttgttgtg gattcgcctt ccattctgct
    21721 ttggttagcc cacctgggtc atctgctttg atatccacac tattgaaaat caggcttaat
    21781 ttgctggctt catcaatgcg tacgcccagt ttggcattgg ctaaattttt ctgtgcgcca
    21841 ctatggtcac gatagccgtg ggtcgtaaaa cgcgtggttg agacggtgta atcaacatcg
    21901 ccggactgtg tgccgtctcc cgttgcgccc gttgctttca gcccatagcg ccagctgcca
    21961 aaactgccgt agtaactact ggcttcaatg gttggtggct gttgtccggt ctgggtggtg
    22021 acattcgtta caccgccaga cgcgttgcca tacagggcag agaaggggcc acgcagcact
    22081 tccacatttt gcacactgct taaatcgatg ttggatgttt gcccttgccc gtcgggcatg
    22141 gtggcgggaa taccgtccac atacaggcga ataccgcgaa ttccgtaagt ggaacgggag
    22201 ccaaatccgc gaatcgacag ctgtaaatct tgcgcatagt tctgccggtt ttgtacctgt
    22261 aaaccaggca cgccggtcag tgattcggac aagttaatgc gcggtgttgc caggcgcatc
    22321 tcctcgccat ccaccacgct tactgctgca ggggtatcca gttctgaaac cacctgcggc
    22381 gcggcactga caatcatggt ctgttcatca gcggcaaaaa caacggggga aaggacaagc
    22441 agtgcgggca aaacggtctg tcggacggaa aaaatcttca tgaaaaaagc caggttaaga
    22501 atgggaaaac gccgtcatgg taatgaaatt gtaaatttat ggaaaatgaa acggcacaat
    22561 acgttaagta attgagaaaa ttgtagtcgt aacggcaaga aatgctccac atttgagaaa
    22621 ataatgatta ccattcccat ttataacaag agcgtaacga tgattacgct tagcgaagca
    22681 ttgtgaagca gcaaaaatat cggttcatca aagggagtcg tcatgcattt acgtcatctg
    22741 ttttcattgc gcctgcgtgg ttcattactg ttaggttcat tgcttgttgc ttcatcattc
    22801 agtacgcagg ccgcagaaga aatgctgcgt aaagcggtag gtaaaggtgc ctacgaaatg
    22861 gcttatagcc agcaagaaaa cgcgctgtgg ctcgccactt cgcaaagccg caaactggat
    22921 aaaggtggcg tggtttatcg tcttgatccg gtcactctgg aagtgacgca ggcgatccat
    22981 aacgatctca agccgtttgg tgccaccatc aataacacga ctcagacgtt gtggtttggt
    23041 aacaccgtaa acagcgcggt cacggcgata gatgccaaaa cgggcgaagt gaaaggccgt
    23101 ctggtgctgg atgatcgtaa gcgcacggaa gaggtgcgcc cgctgcaacc gcgtgagctg
    23161 gtagctgatg atgccacgaa caccgtttac atcagtggta ttggtaaaga gagcgtgatt
    23221 tgggtcgttg atggcgagaa tatcaaactg aaaaccgcca tccagaacac cggtaaaatg
    23281 agtaccggtc tggcgctgga tagcaaaggc aaacgtcttt acaccactaa cgctgacggc
    23341 gaattgatta ccatcgacac cgccgacaat aaaatcctca gccgtaaaaa gctgctggat
    23401 gacggcaaag agcacttctt tatcaacatt agccttgata ccgccaggca gcgtgcattt
    23461 atcaccgatt ctaaagccgc agaagtgtta gtggtcgata cccgtaatgg caatattctg
    23521 gcgaaggttg cggcaccgga atcactggct gtgctgttta accctgcgcg taatgaagcc
    23581 tacgtgacgc atcgtcaggc aggtaaagtc agtgtgattg acgcgaaaag ctataaagtg
    23641 atgaaaacgt tcgatacgcc gactcatcca aacagcctgg cgctgtctgc cgatggcaaa
    23701 acgctgtatg tcagtgtgaa acaaaaatcc actaaacagc aggaagctac ccagccagac
    23761 gatgtgattc gtattgcgct gtaatagata tggcgagggt gaaaacggag agacttgcct
    23821 gacttttatc acgccgcatg cggcaccttc gtcagtaatc taaatttgcc cggcacgtag
    23881 ccgggcaatt ttgcaattaa ctcgccgttt caggcttaaa cacaatttcc tgtttttctt
    23941 catcttcctc gacgactggc gcagtgctgt gaatttcagc aacgcgtttg cgcacaccaa
    24001 accagcctat aaccagcaga ataccgataa tcggcagcgc cgcgatagta taagtcccgt
    24061 tcgggtaatc gaacgccatc agcacaagga cactcagtaa aaacagtaat gtcagccagg
    24121 aagtgaaggg cgcgccaggc agtttaaaac tgacatccgc tgctttgcct tctttaatcg
    24181 ctttacgcag gcgcatctgg cacacgatga taaacgccca tgaagcgatg attcccagcg
    24241 acgcgaagtt caacacaatc tcaaatacgc gcgacggcac cagatagttg aggaatacgc
    24301 cgacgacata cacaaccagt gtcgccagaa tcccggcata cggcacatgc tgacgactca
    24361 ttttcgccat aaaactcggt gcggaaccgc ccatcgccat tgagcgcaga atacgtccgg
    24421 tgcagtacag acctgaattc aggctggaga gggcagcggt cagcaccaca atgttcataa
    24481 tgctgccgat atatggcaca cccagtttag agaaaaacgt cacgaacgga ctttgccccg
    24541 cctgatacgc gctccacggc aataacataa ccagcaacac cacggagccg acgtaaaaca
    24601 ggccaatacg ccaaatcaca ctgttaatcg ctttaggcac catggtctgc ggatctttac
    24661 attctccggc agctgtaccc accatttcaa tggaggcaaa agcaaacact acgccctgaa
    24721 tcaacaccag cgcaggcagc aagccgtggg ggaagaagcc gccattatcg gtgattaaat
    24781 gaaagccagt ggtgttgcca tccagcggct gaccactacc gaggaacact gtacccacga
    24841 ccaaaaaggt cacaatggcg agcactttaa taagcgcaaa ccagaactcc atctccgcaa
    24901 accatttcac accgatcata ttcatggtgc caacgatggt aagtgcagcg agcgcaaaga
    24961 cccactgcgg cacgccgcca aacgcacccc agtaatgcat atacagagcg acggcggtaa
    25021 tatcaacaat ccccgtcatc gcccagttga tgaagtacat ccagccagca acataagcgg
    25081 ctttctcacc caaaaactca cgggcataag agacaaaact gccactggaa gggcggtgta
    25141 gcaccagctc acccaatgca cgcagaataa aaaacgaaaa caagccacaa attaaataaa
    25201 ccagtgccag tgctggaccc gccatttgca gtcgggctcc tgcacctaaa aacaagccgg
    25261 tgccaatcgc gccgccaatg gcgatcatct gtacctggcg attgcccatc gctttgtgat
    25321 acccctcttc gtgggcatta agccagcggc gtttcgcggc gtgttgatct gaagtgtcgg
    25381 tgtcgtgttt actcattgct ctccctgatt gctttaatga aaaagtcata taagttgcca
    25441 tgaacaatgt ttattcatcc tggcgatgat agttatcacc attattcttt atgggtataa
    25501 aactatcggc gcagcatcct acccgttatc gataaacgat gcaaaacatc cccttacaat
    25561 cctgaagggg aataatacaa ctgacgaaaa aatgacaaat ccttttgctg gttaacctgt
    25621 gtactgtcct acacttaatc tttaaaagat tgtgaggggc atatgattaa ggtgtatggc
    25681 gtacccggat ggggctctac aatcagtgag ctgatgctaa cactggctga tattccttat
    25741 cagttcgttg atgtcagtgg ttttgaccac gagggaactt cacgcgagtt attgaaaacc
    25801 ctgaacccgt tgtgtcaggt tccgacactg gctctggaaa atgacgagat tatgacggag
    25861 acagcggcga ttgcattgat ggttctcgag cgtcgcccgg accttgcgcc accggttggg
    25921 cgcgccgaac gtcagcagtt tcaacggcta ttggtctggc tggttgctaa tgtctatcca
    25981 acattcactt tcgctgatta ccctgagcgt tgggctcctg acgcacccga acagttaaag
    26041 aagaatgtta ttgaatatcg taaatcgctt tatatctggc tgaattcaca attgacccct
    26101 gaaccttatg cgtttggtga gcaactaacg ctggtggact gttatctttg cactatgcgc
    26161 acatgggggc ctgggcatga atggtttcag gataatgcca cgaatatcag tgcgattgct
    26221 gatgctgtat gtcagttacc gaaattacaa gaagtattaa aaaggaatgt cattatttga
    26281 gtgggatacc agaagattat gttatttatt attcagtaac tttatccaca atgatgggcg
    26341 t
//
LOCUS       DAZUCE010000048        26064 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZUCE010000048 DAZUCE010000000
VERSION     DAZUCE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.048"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            198..392
                     /gene="bfd"
                     /locus_tag="ACHY03_001683"
     CDS             198..392
                     /gene="bfd"
                     /locus_tag="ACHY03_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFS3904924.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            465..941
                     /gene="bfr"
                     /locus_tag="ACHY03_001684"
     CDS             465..941
                     /gene="bfr"
                     /locus_tag="ACHY03_001684"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFS3904925.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(938..1405)
                     /locus_tag="ACHY03_001685"
     CDS             complement(938..1405)
                     /locus_tag="ACHY03_001685"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFS3904926.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHY03_001686"
     CDS             1784..2095
                     /gene="rpsJ"
                     /locus_tag="ACHY03_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFS3904927.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2128..2757
                     /gene="rplC"
                     /locus_tag="ACHY03_001687"
     CDS             2128..2757
                     /gene="rplC"
                     /locus_tag="ACHY03_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFS3904928.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2768..3373
                     /gene="rplD"
                     /locus_tag="ACHY03_001688"
     CDS             2768..3373
                     /gene="rplD"
                     /locus_tag="ACHY03_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFS3904929.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3370..3672
                     /gene="rplW"
                     /locus_tag="ACHY03_001689"
     CDS             3370..3672
                     /gene="rplW"
                     /locus_tag="ACHY03_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFS3904930.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3690..4511
                     /gene="rplB"
                     /locus_tag="ACHY03_001690"
     CDS             3690..4511
                     /gene="rplB"
                     /locus_tag="ACHY03_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFS3904931.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHY03_001691"
     CDS             4528..4806
                     /gene="rpsS"
                     /locus_tag="ACHY03_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFS3904932.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4821..5153
                     /gene="rplV"
                     /locus_tag="ACHY03_001692"
     CDS             4821..5153
                     /gene="rplV"
                     /locus_tag="ACHY03_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFS3904933.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHY03_001693"
     CDS             5171..5872
                     /gene="rpsC"
                     /locus_tag="ACHY03_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFS3904934.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5885..6295
                     /gene="rplP"
                     /locus_tag="ACHY03_001694"
     CDS             5885..6295
                     /gene="rplP"
                     /locus_tag="ACHY03_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFS3904935.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHY03_001695"
     CDS             6295..6486
                     /gene="rpmC"
                     /locus_tag="ACHY03_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFS3904936.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHY03_001696"
     CDS             6486..6740
                     /gene="rpsQ"
                     /locus_tag="ACHY03_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFS3904937.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6905..7276
                     /gene="rplN"
                     /locus_tag="ACHY03_001697"
     CDS             6905..7276
                     /gene="rplN"
                     /locus_tag="ACHY03_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFS3904938.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7287..7601
                     /gene="rplX"
                     /locus_tag="ACHY03_001698"
     CDS             7287..7601
                     /gene="rplX"
                     /locus_tag="ACHY03_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFS3904939.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7616..8155
                     /gene="rplE"
                     /locus_tag="ACHY03_001699"
     CDS             7616..8155
                     /gene="rplE"
                     /locus_tag="ACHY03_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFS3904940.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHY03_001700"
     CDS             8170..8475
                     /gene="rpsN"
                     /locus_tag="ACHY03_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFS3904941.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHY03_001701"
     CDS             8509..8901
                     /gene="rpsH"
                     /locus_tag="ACHY03_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFS3904942.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8914..9447
                     /gene="rplF"
                     /locus_tag="ACHY03_001702"
     CDS             8914..9447
                     /gene="rplF"
                     /locus_tag="ACHY03_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFS3904943.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9457..9810
                     /gene="rplR"
                     /locus_tag="ACHY03_001703"
     CDS             9457..9810
                     /gene="rplR"
                     /locus_tag="ACHY03_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFS3904944.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHY03_001704"
     CDS             9825..10328
                     /gene="rpsE"
                     /locus_tag="ACHY03_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFS3904945.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHY03_001705"
     CDS             10332..10511
                     /gene="rpmD"
                     /locus_tag="ACHY03_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFS3904946.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10515..10949
                     /gene="rplO"
                     /locus_tag="ACHY03_001706"
     CDS             10515..10949
                     /gene="rplO"
                     /locus_tag="ACHY03_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFS3904947.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10957..12288
                     /gene="secY"
                     /locus_tag="ACHY03_001707"
     CDS             10957..12288
                     /gene="secY"
                     /locus_tag="ACHY03_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFS3904948.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHY03_001708"
     CDS             12320..12436
                     /gene="rpmJ"
                     /locus_tag="ACHY03_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFS3904949.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHY03_001709"
     CDS             12583..12939
                     /gene="rpsM"
                     /locus_tag="ACHY03_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFS3904950.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHY03_001710"
     CDS             12956..13345
                     /gene="rpsK"
                     /locus_tag="ACHY03_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFS3904951.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHY03_001711"
     CDS             13379..13999
                     /gene="rpsD"
                     /locus_tag="ACHY03_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFS3904952.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHY03_001712"
     CDS             14025..15014
                     /gene="rpoA"
                     /locus_tag="ACHY03_001712"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFS3904953.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHY03_001713"
     CDS             15055..15438
                     /gene="rplQ"
                     /locus_tag="ACHY03_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFS3904954.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15545..15913
                     /locus_tag="ACHY03_001714"
     CDS             15545..15913
                     /locus_tag="ACHY03_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFS3904955.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15924..16349
                     /gene="zntR"
                     /locus_tag="ACHY03_001715"
     CDS             15924..16349
                     /gene="zntR"
                     /locus_tag="ACHY03_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFS3904956.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16405..16623
                     /gene="arfA"
                     /locus_tag="ACHY03_001716"
     CDS             16405..16623
                     /gene="arfA"
                     /locus_tag="ACHY03_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFS3904957.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHY03_001717"
     CDS             complement(16620..17030)
                     /gene="mscL"
                     /locus_tag="ACHY03_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFS3904958.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHY03_001718"
     CDS             complement(17160..18536)
                     /gene="trkA"
                     /locus_tag="ACHY03_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFS3904959.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHY03_001719"
     CDS             complement(18558..19847)
                     /gene="rsmB"
                     /locus_tag="ACHY03_001719"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFS3904960.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHY03_001720"
     CDS             complement(19893..20840)
                     /gene="fmt"
                     /locus_tag="ACHY03_001720"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFS3904961.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHY03_001721"
     CDS             complement(20855..21364)
                     /gene="def"
                     /locus_tag="ACHY03_001721"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFS3904962.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21494..22618
                     /gene="dprA"
                     /locus_tag="ACHY03_001722"
     CDS             21494..22618
                     /gene="dprA"
                     /locus_tag="ACHY03_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFS3904963.1"
                     /translation="MVDTEIWLRLMSVSSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22590..23063
                     /gene="smg"
                     /locus_tag="ACHY03_001723"
     CDS             22590..23063
                     /gene="smg"
                     /locus_tag="ACHY03_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFS3904964.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23092..23634
                     /locus_tag="ACHY03_001724"
     CDS             23092..23634
                     /locus_tag="ACHY03_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFS3904965.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHY03_001725"
     CDS             23639..24211
                     /gene="tsaC"
                     /locus_tag="ACHY03_001725"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFS3904966.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAIRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24216..25034
                     /gene="aroE"
                     /locus_tag="ACHY03_001726"
     CDS             24216..25034
                     /gene="aroE"
                     /locus_tag="ACHY03_001726"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFS3904967.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQTLSMGELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25031..25288
                     /locus_tag="ACHY03_001727"
     CDS             25031..25288
                     /locus_tag="ACHY03_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFS3904968.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25264..25818)
                     /locus_tag="ACHY03_001728"
     CDS             complement(25264..25818)
                     /locus_tag="ACHY03_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFS3904969.1"
                     /translation="MSDILRPYRDLFPQIGQRVMIDGSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6697 a         6176 c         6752 g         6439 t
ORIGIN      
        1 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
       61 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      121 ggagcaaata aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa
      181 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      241 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      301 tgggaaatca atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga
      361 tgcagatgcc agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc
      421 tagccccatc tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta
      481 aaatcataaa ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt
      541 ttctccatgc ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc
      601 atgaatccat cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag
      661 aaggaatccc caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga
      721 tgctgcaatc cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg
      781 cgtatgccga cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg
      841 atgaagaagg tcatattgac tggctcgaaa cagagttaga tctcattggc aaaattggcc
      901 tgcaaaatta cctgcaatca caaattaaag ttaaagatta acggcctatc agcaaactat
      961 cccagcctat aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta
     1021 atgattcttt tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag
     1081 caaggaaaac cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa
     1141 acttcacatc accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa
     1201 tgacagcgaa tgtgccgtaa ccaataattg ccccccataa cgcatcggct aaaccatcag
     1261 gatgacaaac ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag
     1321 gtaacaagcc atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa
     1381 ttagaaaggg aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa
     1441 taactccgga tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa
     1501 acgctatctt cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga
     1561 ttaatttcta cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg
     1621 tagttgacag caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct
     1681 cccaatcggg gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag
     1741 gagtaatcat tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat
     1801 ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt
     1861 cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa
     1921 agagcgcttc actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga
     1981 aatccgtact cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc
     2041 tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg
     2101 tcattgagcg attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg
     2161 acccgtatct tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca
     2221 aaccgcgtta ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc
     2281 accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct
     2341 ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact
     2401 gtaggtcaga gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc
     2461 acctctaaag gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaggac
     2521 gctactcacg gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact
     2581 ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc
     2641 gttcagagcc ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt
     2701 gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga
     2761 gatagcaatg gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac
     2821 cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg
     2881 tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa
     2941 accgtggcgc cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg
     3001 gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa
     3061 gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct
     3121 gatcgttgtc gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact
     3181 gaaagacatg gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt
     3241 cctggctgcg cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt
     3301 tagcctgatc gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga
     3361 gatgctggca tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct
     3421 gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac
     3481 gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc
     3541 gttaacaccc tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt
     3601 agcgactgga aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc
     3661 ggcgctgagt aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc
     3721 cgggtcgtcg ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg
     3781 ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca
     3841 ctcgtcatat cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca
     3901 aagacggtat cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca
     3961 tcgcgctggt tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga
     4021 aagctggcga ccagattcag tctggcgttg atgctgcaat caaaccaggt aacaccctgc
     4081 cgatgcgcaa catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag
     4141 gcggtcagct ggcacgttcc gctggtactt acgttcagat cgttgctcgt gatggtgctt
     4201 atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa
     4261 ctctgggcga agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg
     4321 cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc
     4381 cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc
     4441 agaccaaagg taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc
     4501 gtagcaaata attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt
     4561 gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg
     4621 cgcacttggt cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc
     4681 cataatggtc gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg
     4741 ggtgaattcg caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag
     4801 aaataaggta ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg
     4861 ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg
     4921 atattctgac ctacaccaac aagaaagcgg ctgtactggt caagaaggtt ctggaatctg
     4981 ccattgctaa cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa
     5041 ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag
     5101 atcgcatcct gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg
     5161 gagactagca atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc
     5221 atggaactct acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt
     5281 taaagtacgt cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat
     5341 cgagcgtccg gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatagttat
     5401 cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt
     5461 tcctgcacag atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc
     5521 tgacagcatc acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc
     5581 tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct
     5641 gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac
     5701 tctgcgtgct gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg
     5761 cgttaaagtg tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc
     5821 ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc
     5881 gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg
     5941 tctggcgcag ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg
     6001 tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg
     6061 tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc
     6121 agtgcgtatg ggtaaaggta aaggtaacgt ggagtactgg gttgccttga ttcagccggg
     6181 taaagtcctg tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct
     6241 ggcagcagcg aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa
     6301 gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg
     6361 cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg
     6421 ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt
     6481 gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga
     6541 aatccattgt tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca
     6601 agcgtacgac caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg
     6661 ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg
     6721 tagagaaagc ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat
     6781 gagccgttta ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat
     6841 atggggattt ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac
     6901 taaaatgatc caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt
     6961 aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat
     7021 caagatcacc atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa
     7081 ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt
     7141 cgatggtaat gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat
     7201 ttttgggccg gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc
     7261 accagaagta ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta
     7321 tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg
     7381 gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc
     7441 tgaaccaacc gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa
     7501 tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa
     7561 aagtccgttt cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc
     7621 gaaactgcat gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta
     7681 caattctgtc atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga
     7741 agcgatcgct gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg
     7801 tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg
     7861 ctatccgatc ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg
     7921 cctgatcact attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt
     7981 cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga
     8041 ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc
     8101 tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag
     8161 ggttactaaa tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct
     8221 gataaatact tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc
     8281 gacgaagatc gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg
     8341 tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc
     8401 gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa
     8461 aaggctagct ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa
     8521 gatccgatcg cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg
     8581 gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt
     8641 tttattgaag attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag
     8701 tatttccagg gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc
     8761 atctataaac gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt
     8821 tctacctcta aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa
     8881 attatctgct acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc
     8941 gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa
     9001 aacggcgagc tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc
     9061 ctgaccttcg gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt
     9121 gccctgctga actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg
     9181 gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc
     9241 tctcatcctg ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact
     9301 gaaatcgtgc tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc
     9361 gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg
     9421 cgtaccaaag aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc
     9481 cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta
     9541 catcgtaccc cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg
     9601 gtagctgctt ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa
     9661 gacgcggctg cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa
     9721 gatgtatcct ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat
     9781 gctgcccgtg aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa
     9841 aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta
     9901 aaggtggtcg tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg
     9961 ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa
    10021 aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta
    10081 aaggtgttca cacgggttct cgcgtattca tgcagccggc ttccgaaggt accggtatca
    10141 tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta
    10201 aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa
    10261 atatgaattc tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg
    10321 ggaaataaac catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc
    10381 tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag
    10441 agcgcgagga tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag
    10501 ttgaggagta agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg
    10561 gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca
    10621 aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc
    10681 ctctgtaccg tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg
    10741 aagttcgtct gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga
    10801 aagcggctaa cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag
    10861 taacgactcc ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg
    10921 aagctgctgg cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat
    10981 tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt
    11041 gcgctgattg tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta
    11101 cttgccaaac tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct
    11161 ggtggtgctc tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg
    11221 tcgatcatta tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa
    11281 ggggagtctg gtcgtcgtaa gatcagccag tacacccgct acggtactct ggtgctggca
    11341 atattccagt cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg
    11401 gtgattaacc cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg
    11461 atgttcctga tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca
    11521 atcattatct tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag
    11581 caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt
    11641 gcagtgacgt tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac
    11701 gcgaaacgtc agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa
    11761 gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg
    11821 accatcgcgt catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg
    11881 tatttgcagc ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc
    11941 tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag
    12001 tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa
    12061 gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg
    12121 gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc
    12181 gttgttgtcg tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag
    12241 tatgagtctg cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga
    12301 gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg
    12361 caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa
    12421 acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag
    12481 attagccagc caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc
    12541 ttttcagcat ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc
    12601 attaacattc ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc
    12661 aagacccgtt ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt
    12721 gagctgtctg aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa
    12781 ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat
    12841 cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca
    12901 cgtacccgta agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc
    12961 aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca
    13021 tatccatgct tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt
    13081 gggttgggca acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc
    13141 agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct
    13201 ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc
    13261 cgcaggtttc cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg
    13321 tccgccgaaa aaacgtcgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat
    13381 ggcaagatat ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct
    13441 taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca
    13501 cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt
    13561 tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg
    13621 tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt
    13681 tgtataccgt atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa
    13741 agcaattatg gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga
    13801 cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct
    13861 ggctgagcag cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac
    13921 gtttaagcgt aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt
    13981 cgagctttac tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga
    14041 cagagtttct aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg
    14101 tgacccttga gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta
    14161 ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac
    14221 atgagtacag caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga
    14281 aagggctggc ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg
    14341 gcattggccc tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc
    14401 cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag
    14461 ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc
    14521 gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca
    14581 atgttgaagc agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg
    14641 aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg
    14701 aacaactgga agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga
    14761 aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct
    14821 ctgctaactg ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg
    14881 aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg
    14941 tgctggcttc ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca
    15001 tcgctgacga gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc
    15061 catcgtaaga gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc
    15121 aatatggcag gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa
    15181 gagctgcgcc gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat
    15241 cgtcgtctgg cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa
    15301 ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc
    15361 cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg ttgatcgttc agagaaagca
    15421 gaagctgctg cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta
    15481 tacccgtagc atccccactt atctacaata gctgtactct ttttgttcat cccctggagt
    15541 atttatgtgg ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg
    15601 tgagtttgat aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt
    15661 gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct tacctccaga
    15721 acttgagcaa cggagggaag caattcagct tctggatatc ctcaaaggta tccgtcacga
    15781 tgatccgcaa tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca
    15841 agctggattg agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat
    15901 caacgataac tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag
    15961 taacacccga cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta
    16021 ctgaaggtgg gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc
    16081 atgccagaca actaggtttc agtctggagt cgatccgcga gttgctgtcg atccgtatcg
    16141 atcctgaaca ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag
    16201 tcgaagcacg gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg
    16261 cctgttgtgg gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag
    16321 gggcgagtgg cgttaagagt ggctgttgat tttttgcact ggcaggatta cactcgcgcc
    16381 gttaaataac caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag
    16441 gataatgcga tagaagcatt actacatgat cccttattcc gacagcgagt agagaaaaat
    16501 aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag
    16561 gctagtggca agaaagtaaa tcactttttt accactggcc ttctgctttc aggtgtttgt
    16621 taagagcggt tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct
    16681 ttagttggtg ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc
    16741 ttaatcgcca taaagatggc aaaggctaca atcagaaaat caaagacgtt ttgaatgaag
    16801 acaccgtaat gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca
    16861 aactgtttaa agtcgatccc gccaattaat aagcccagtg gtggcatgat gatatcggca
    16921 accagtgaag atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc
    16981 accacgttcc cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc
    17041 ctatgaatcc gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa
    17101 tttaattaat ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat
    17161 tacaagaaga aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ctttttatct
    17221 gtgaggaaca taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc
    17281 acgtcgtttc cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca
    17341 atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct
    17401 tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta
    17461 agcaacgcag aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga
    17521 accagatcca cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt
    17581 ttggcaagca tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga
    17641 tcaacttgat cgatatgttc ttcagccagc agttcttgat ccgacgcatc accaaaaaag
    17701 acgatcgtat tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg
    17761 agtttaacgc tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca
    17821 ccaaccagca tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg
    17881 cggatatgct gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa
    17941 ccttgcggac gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgg
    18001 ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct
    18061 ttaaccacag ccaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga
    18121 tactcaatca gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca
    18181 atcggcacag catctgaatg aaatagctta tcggcatcgc gcacgtagtc tggtgagcgg
    18241 atacgagcga tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata
    18301 ttggtttcat ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc
    18361 cgcaatacgc gtggatgaga gccatgcccc tgcacgaccc gcaggtcaaa tttatcctgt
    18421 aaggtccgca gacgctcacc gttggtatcg acaacagtaa tatcgttgtt ctcgccaacc
    18481 aggttttccg ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt
    18541 cgcgacccgt tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct
    18601 tcggcaccag gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg
    18661 gcatcagcgg tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc
    18721 gaacaggtgg catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga
    18781 atttcagact gcaattgcgc gagttctggg atatcgcgat cgcgacgtaa ccatttaata
    18841 tccggatggc gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca
    18901 aactgttgct cgccacacca ttgagaaggg taacggccat caccttgttt cacggtcgcc
    18961 ttcataccaa ggcgttttaa attgtcgtaa acccgagaga ggcgctgttc gtcgatatca
    19021 accgcaacaa cctgcgcttc tggtgccacc tcaaggatat gcgttgtttt accgccgggg
    19081 gcggcacaaa gatccaaaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct
    19141 tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca
    19201 ggtgcaggtg tttccagacg tacagcatca gggtaatccg catgcgggaa acctttcatt
    19261 cctgcttcat ccagcaatgc aagccagctg tcgcgggaat gatgcgtacg attaacgcgc
    19321 agccacattg gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac
    19381 gctttttgca gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggcatta
    19441 aactcggcta ataactcttc ttgctgacgc tggaactggc gtaatacacc gtttatcaac
    19501 cctttaagtt gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca
    19561 tgaggtggaa tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcacg
    19621 gtccgctgtt tgccggtcat cggacgggcc attaacttat taatcagcca gtctaactgc
    19681 gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa
    19741 actttttgct ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt
    19801 tcaacggcct gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg
    19861 cccggcaaga ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac
    19921 cattcccgac gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt
    19981 aacgagagca ggttcaggat gccatcaccc gtcgcaacct gaatgccttg tttgttggct
    20041 tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact
    20101 ttaaccggct gtccttcaat ttctacccag ctcattggcc atggattgaa agcgcgaatg
    20161 cagcgttcaa gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac
    20221 ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc
    20281 aattgtttca acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc
    20341 gtaccactgg tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg
    20401 tctaaaccaa catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt
    20461 gagcgttgga ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata
    20521 cagccaagac gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca
    20581 accataacat cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa
    20641 acaggttgaa aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc
    20701 agttttttac cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga
    20761 ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata
    20821 atacgtagtg attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca
    20881 gtttttcaac tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca
    20941 gtttgccgac caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgctt
    21001 ccagttcaaa tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg
    21061 gcactaaagc acgttgttca gggatcgaca ggcatccttc ttcaatgcct gtttcgccgc
    21121 ttttttctaa aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat
    21181 caataacaat gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt
    21241 ctgcgtacat cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt
    21301 ctaccggttt agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg
    21361 acataaatct ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa
    21421 atccccctct gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg
    21481 cagggagata aggatggtcg atacagaaat ttggctgcgt ttaatgagcg tcagcagctt
    21541 gtacggcgat gatatggtcc gtatcgctca ctggctggca aaacagtcgc atattgatgc
    21601 ggttgtattg cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc
    21661 acgaaagagt atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc
    21721 tgcggacagc gaattttatc ctcctcaact tctggcgacg acagattacc ccggcgcact
    21781 gtttgttgaa ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg
    21841 ggcgcattca tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagcg
    21901 tggagtgaca attacgagtg gactggcgcg tggaatcgat ggtgtggcgc ataaagcggc
    21961 cttacaggta aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc
    22021 gcgtcgccat gcccgactgg ctaccagttt gcttgaacat ggtggggcac ttgtctcgga
    22081 atttcccctc gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag
    22141 tggtctaagt aaaggtgtac tggtggtgga agcggctttg cgcagtggtt cgctggtgac
    22201 agcacgttgt gcgcttgagc aggggcgtga agtttttgcc ctgccaggtc cattagggaa
    22261 tccgggaagc gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc
    22321 ggaagaaatt ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa
    22381 ttcattttat tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa
    22441 cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc
    22501 agaggtagtt actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg
    22561 cggctatgtc cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa
    22621 cctatattca cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta
    22681 ccgacgcagg atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg
    22741 cggattatca ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc
    22801 gtatttatac ccctgaggag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt
    22861 tccttgagca aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc
    22921 tcgcgctgga taccgcagag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt
    22981 tgttcaatat tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag
    23041 tgaatgaagg tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa
    23101 tcagcactgt tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt
    23161 attcgatccg ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac
    23221 gtccgtcctc tgaaatcttc agcggatgga catatcgtca aagttctgga ggggcaggtt
    23281 tgccctgcat gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt
    23341 tgcagtaact accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca
    23401 attacatgtc cccaatgccg gacgggccat ctggtccagc gccgctcccg ttatggcaaa
    23461 acatttcact cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata
    23521 gctggagaat gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt
    23581 gtaaaacact tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt
    23641 gaataataac ctgcaaggag acgctatcgc agctgcgata gatgtcctca atgaagaacg
    23701 tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac
    23761 agcagtgatg cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat
    23821 cgcagcaaat tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgtgca
    23881 gcgtgaaacc attttttccc gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc
    23941 gacaacaccg cgctggttga cgggccgctt tgattcgctt gctatacgag tcaccgacca
    24001 tccgttggtg gttgctttgt gccaggctta tggtaaaccg ctggtttcta ccagtgccaa
    24061 cttgagtgga ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc
    24121 gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc
    24181 cctgacgggt gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta
    24241 atccgatagc ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata
    24301 ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg
    24361 ctttctttag tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt
    24421 ttgccagagc ggatgagctt actgaacggg cagcgttggc tggagcagtt aataccctca
    24481 agcgtttaga agatggacgc ctgctggggg acaataccga tggtataggc ttgttaagcg
    24541 atctggaacg tctgtctttt atccgtcctg gtttacgtat tctgctcatc ggcgctggtg
    24601 gagcatctcg cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacca
    24661 atcgaacggt atcccgcgcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc
    24721 agacgttgag tatgggcgaa ctggaaggtc atgagtttga tctcattatt aatgccacat
    24781 ccagtggcat cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt
    24841 attgctatga catgttctat cagaaaggaa aaactccttt tctggcatgg tgtgagcagc
    24901 gaggctcaaa gcgtaatgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct
    24961 tccttctttg gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg
    25021 aattgtccgc gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa
    25081 aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgtg cgatctctgg
    25141 cgatagcctg gcgtatcgct ttactggaga tacgccagag cagtggttag cgagttttcg
    25201 tcagcatcgc tgggacctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga
    25261 tgatcaaggt tgggtctggt taccctgatc caggtattcg tctttccatt tcacgtaatt
    25321 attcgcggaa taacgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac
    25381 gggactaccg agatacagat atccgctctc cagccgttta ttttgtggga ccaggctacc
    25441 cgcaccaatc atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc
    25501 aaccaaaact cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac
    25561 atcttcgcca atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg
    25621 caacatactg ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc
    25681 acgaatcaca acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac
    25741 gacactgcta ccgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg
    25801 gcgtaaaata tcagacatac ttacctcaac aataaatgat ttactaatga ctttgggtac
    25861 attattggcc ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgaatgc
    25921 agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc aaaaaagtac ttgtgcaaaa
    25981 aattgggatc cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaagatg
    26041 gtcgtaacaa cgaagagaaa aaat
//
LOCUS       DAZUCE010000049        26013 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZUCE010000049 DAZUCE010000000
VERSION     DAZUCE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.049"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            54..169
                     /gene="rrf"
                     /locus_tag="ACHY03_001729"
     rRNA            54..169
                     /gene="rrf"
                     /locus_tag="ACHY03_001729"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            495..1793
                     /gene="kgtP"
                     /locus_tag="ACHY03_001730"
     CDS             495..1793
                     /gene="kgtP"
                     /locus_tag="ACHY03_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFS3904970.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1790..2113)
                     /gene="yfiM"
                     /locus_tag="ACHY03_001731"
     CDS             complement(1790..2113)
                     /gene="yfiM"
                     /locus_tag="ACHY03_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFS3904971.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2159..3514)
                     /gene="pssA"
                     /locus_tag="ACHY03_001732"
     CDS             complement(2159..3514)
                     /gene="pssA"
                     /locus_tag="ACHY03_001732"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFS3904972.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3628..6288)
                     /gene="pat"
                     /locus_tag="ACHY03_001733"
     CDS             complement(3628..6288)
                     /gene="pat"
                     /locus_tag="ACHY03_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFS3904973.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEDDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6320..7018)
                     /gene="tapT"
                     /locus_tag="ACHY03_001734"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6320..7018)
                     /gene="tapT"
                     /locus_tag="ACHY03_001734"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFS3904974.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7087..7506)
                     /gene="trxC"
                     /locus_tag="ACHY03_001735"
     CDS             complement(7087..7506)
                     /gene="trxC"
                     /locus_tag="ACHY03_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFS3904975.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7713..8750
                     /gene="yfiF"
                     /locus_tag="ACHY03_001736"
     CDS             7713..8750
                     /gene="yfiF"
                     /locus_tag="ACHY03_001736"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFS3904976.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8798..9487)
                     /gene="ung"
                     /locus_tag="ACHY03_001737"
     CDS             complement(8798..9487)
                     /gene="ung"
                     /locus_tag="ACHY03_001737"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFS3904977.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9792..10175
                     /gene="grcA"
                     /locus_tag="ACHY03_001738"
     CDS             9792..10175
                     /gene="grcA"
                     /locus_tag="ACHY03_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFS3904978.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10231..10818)
                     /gene="eamB"
                     /locus_tag="ACHY03_001739"
     CDS             complement(10231..10818)
                     /gene="eamB"
                     /locus_tag="ACHY03_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFS3904979.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            10921..11802
                     /gene="yfiE"
                     /locus_tag="ACHY03_001740"
     CDS             10921..11802
                     /gene="yfiE"
                     /locus_tag="ACHY03_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3904980.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNGCSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEEYFVVA"
     gene            complement(11837..13171)
                     /gene="srmB"
                     /locus_tag="ACHY03_001741"
     CDS             complement(11837..13171)
                     /gene="srmB"
                     /locus_tag="ACHY03_001741"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFS3904981.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13303..14040
                     /gene="trmN"
                     /locus_tag="ACHY03_001742"
     CDS             13303..14040
                     /gene="trmN"
                     /locus_tag="ACHY03_001742"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFS3904982.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     IKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAASQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYQQGVECSTPQREQARYTTTLDHPSLLACAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14025..15647)
                     /gene="nadB"
                     /locus_tag="ACHY03_001743"
     CDS             complement(14025..15647)
                     /gene="nadB"
                     /locus_tag="ACHY03_001743"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFS3904983.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKEKVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDVRGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15903..16058
                     /gene="rseD"
                     /locus_tag="ACHY03_001744"
     CDS             15903..16058
                     /gene="rseD"
                     /locus_tag="ACHY03_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFS3904984.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            16055..16630
                     /gene="rpoE"
                     /locus_tag="ACHY03_001745"
     CDS             16055..16630
                     /gene="rpoE"
                     /locus_tag="ACHY03_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFS3904985.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16663..17313
                     /gene="rseA"
                     /locus_tag="ACHY03_001746"
     CDS             16663..17313
                     /gene="rseA"
                     /locus_tag="ACHY03_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFS3904986.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17313..18269
                     /gene="rseB"
                     /locus_tag="ACHY03_001747"
     CDS             17313..18269
                     /gene="rseB"
                     /locus_tag="ACHY03_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFS3904987.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18266..18745
                     /gene="rseC"
                     /locus_tag="ACHY03_001748"
     CDS             18266..18745
                     /gene="rseC"
                     /locus_tag="ACHY03_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFS3904988.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18943..20742
                     /gene="lepA"
                     /locus_tag="ACHY03_001749"
     CDS             18943..20742
                     /gene="lepA"
                     /locus_tag="ACHY03_001749"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFS3904989.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20758..21732
                     /gene="lepB"
                     /locus_tag="ACHY03_001750"
     CDS             20758..21732
                     /gene="lepB"
                     /locus_tag="ACHY03_001750"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFS3904990.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            22005..22685
                     /gene="rnc"
                     /locus_tag="ACHY03_001751"
     CDS             22005..22685
                     /gene="rnc"
                     /locus_tag="ACHY03_001751"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFS3904991.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22682..23587
                     /gene="era"
                     /locus_tag="ACHY03_001752"
     CDS             22682..23587
                     /gene="era"
                     /locus_tag="ACHY03_001752"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFS3904992.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23599..24327
                     /gene="recO"
                     /locus_tag="ACHY03_001753"
     CDS             23599..24327
                     /gene="recO"
                     /locus_tag="ACHY03_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFS3904993.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGGTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24339..25070
                     /gene="pdxJ"
                     /locus_tag="ACHY03_001754"
     CDS             24339..25070
                     /gene="pdxJ"
                     /locus_tag="ACHY03_001754"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFS3904994.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25070..25450
                     /gene="acpS"
                     /locus_tag="ACHY03_001755"
     CDS             25070..25450
                     /gene="acpS"
                     /locus_tag="ACHY03_001755"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS3904995.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            25871..25951
                     /gene="shoB"
                     /locus_tag="ACHY03_001756"
     CDS             25871..25951
                     /gene="shoB"
                     /locus_tag="ACHY03_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFS3904996.1"
                     /translation="MTDCRYLIKRAIKIIIAVLQLILLFL"
BASE COUNT         6361 a         6508 c         6883 g         6261 t
ORIGIN      
        1 gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg
       61 gccgtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg
      121 ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaag
      181 cgaaaggcca tcctgacgga tggccttttt gcattggcgc agaaaaaaat acctgatgcg
      241 acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg gtttccccaa
      301 cttgcctact tccatacgtg tcctccttac cagaaattta tccttaagct cctcaataac
      361 cattttcctg ctaactaaat tcatggttaa ggttgcataa tgatacgcaa caaatgtata
      421 acattttctt taccaaaaga aataaacaaa agcgaccgac aaaagcatcg gattacggca
      481 ggagacataa tggcatggct gaaagtactg taacggcaga cagcaaactg acaagtagtg
      541 atactcgtcg ccgcatttgg gcgattgtgg gggcctcttc aggtaatctg gtcgagtggt
      601 tcgatttcta tgtctactcg ttctgttcac tctactttgc ccacatcttc ttcccttccg
      661 ggaacacgac gactcaacta ctacaaacag caggtgtttt tgctgcggga ttcctgatgc
      721 gcccaatagg cggttggcta tttggccgca tagccgataa acatggtcgc aaaaaatcga
      781 tgctgctatc ggtgtgtatg atgtgtttcg gctcactggt aatcgcctgt ttgcctggtt
      841 atgaaacaat cggtacgtgg gctccggcat tattgcttct cgcgcgttta tttcagggat
      901 tatccgttgg cggagaatat ggcaccagcg ccacctatat gagtgaagtt gccgttgaag
      961 ggcgcaaagg tttttacgca tcatttcagt atgtgacgtt gatcggcgga caactgctag
     1021 ccctactggt tgtcgtggtt ttacaacaca ccatggaaga cgctgcactc agagagtggg
     1081 gatggcgtat tcctttcgcg ttaggagctg tgttagctgt tgtggcgttg tggttacgtc
     1141 gtcagttaga tgaaacttcg caacaagaaa cgcgcgcttt aaaagaagct ggatctctga
     1201 aaggattatg gcgcaatcgc cgtgcattca tcatggttct cggttttacc gctgcgggct
     1261 ccctttgttt ctataccttc accacttata tgcagaagta tctggtaaat actgcgggaa
     1321 tgcatgccaa cgtggcgagt ggcattatga ctgccgcatt gtttgtattc atgcttattc
     1381 aaccactcat tggcgcgctg tcggataaga ttggtcgccg tacctcaatg ttatgtttcg
     1441 gttcgctggc agcgattttt accgttccta ttctctcagc attgcaaaac gtttcctcgc
     1501 cttatgccgc ttttggtctg gtgatgtgtg ccctgctgat agtgagtttt tatacatcaa
     1561 tcagtggaat actgaaggct gagatgttcc cggcacaggt tcgcgcatta ggcgttggtc
     1621 tgtcatatgc ggtcgctaat gctatatttg gtggttcggc ggagtacgta gcgttgtcgc
     1681 tgaaatcaat aggaatggaa acagccttct tctggtatgt gaccttgatg gccgtggtgg
     1741 cgtttctggt ttctttgatg ctacatcgca aagggaaggg gatgcgtctt tagtgacggg
     1801 tcagttgcca gacggtatag ccggtgcttg caccggcgac atcccaggcc aaatccttcc
     1861 agctccagcc gctcccttcg gggcggctat cccaaagctc ttttgacgcc cccaaactga
     1921 cagagaacat caatccaaac atggcactgc gatcccggct catcccctga tgctgtgaat
     1981 attcatttcc ggcggcggaa agcatcgccg aggcgataaa gtgttgagct ttatcctgcc
     2041 cgctccagct atcgttagcc atatggctgc atccagaaag taacaatagc gaacagacaa
     2101 aaagaatacg caaaacgtgg cctccaaaaa acaaaccccg tacaggacgg ggttgtgatt
     2161 acaggatgcg gctaattaat cggtcgatgc ggatacggcg caaacggcgg atgagtttac
     2221 gaaccttcac cggataatcg gcaatacttt gcagatcgcg atagtgctta acgatggtgg
     2281 tatgctcgcg gatcagctcc agttctttct ctcgctgtgg cgcaagctcc agttgcggat
     2341 cgtggatcaa aatggcgttt tccagatcca gacgccaggc gcgcgggttc aggttattac
     2401 cggtgatcaa catccactta tcatcaaccc acatcccttt caggtgatag gtgttgtcgt
     2461 catctttcca taaccgaacc actaactggt cagtattgac gtaatactgc aaacggctca
     2521 ggaaacgacg cagattgatc tcatagagat aaggcaatgc gccaattatc ttgaaaggtt
     2581 catcttccgg aatgtagaag tcgttcgcgg ttttatcacc aacaataatt tcgacctttt
     2641 tcccttcgcg cagcaactgg ataatattgc gcacaaggat tgctggcagg ttgaagtatg
     2701 gcgtacagat ggttagtttc tgctcggcac aaggcataag atggaaaatg gtcttgttca
     2761 acagactcga tttccccagc cccactagcg gcgttacgga aagctgatcg ttgtcggcat
     2821 cgccctggaa atgataagcg gcatcacgca gctcctggcg gaacagacga atatcattct
     2881 tgatttccgg gctttttggc cgattaacat catccagacg attaacgccg cggccattca
     2941 taatattctg tgtaacccat tcaaacataa tgtctgacat cttacggtta cggataagat
     3001 gataacggtc gtagcgatat ttatcgtgct gatgcaggta aacatcgttc aggctggcac
     3061 cgctataaag tacgctatcg tcgatgataa agcctttaaa gtgcagaaca ccaagggctt
     3121 cacgggtatt gattggaacg ccataaaccg gaacatctac gcccggattt tcctgcgcca
     3181 tgcggcagta ccagtcagcg ttagtgttag atgccgcagc gccaatgcgt ccacgttgtg
     3241 cacgatgcca gtcgaccagc acccgcacat ccagttccgg acgctgcctt ttagcttcat
     3301 acaacgcgtt cagaatgcct ttgccaccgt catcctgttc gagatacagg gcgacaatgc
     3361 aaatgcgctg cttcgcgctg gctatttttt ccagcagcgt ctcccggaaa tcggcgggag
     3421 cgtaaaagaa atcgacatca tcaactgatt gagaaatctt gggtagttgg gcaaggtgtt
     3481 gttgatgttt attacgctta aattttgaca acatcacagt gcatttcttc tctgttcatt
     3541 gaagggtcct ctgtgcaatg cagacgacat aagcgggcaa taataacacc agtcccgatt
     3601 aagtggtcaa catttccagt accttactca tgattcctcg cgctgggcaa gatttagcgt
     3661 aagcccaacg atcccctctt cgagctggat atcaacgtta aacccgagct tgcgggccag
     3721 cgccaccatg ccacgattat ttggcatcgt aataccattc agacgttgta gtccgtgatc
     3781 tcgcgtatag gtaatcaact tttccattaa gcgtcgacct aagcctaacc ctttgagatc
     3841 cgagcgaacc agcacggcaa attcggcatc gatgttatca gggtcggaga tcgcacgcgt
     3901 gacgccgagg atctcttccg tttgatcaat acgtcgtacc gctacaaacg ccatttcccg
     3961 atcgtagtcg atctgcgtca tgttggctaa atcttcatgg gtaaattcgt tgatctcact
     4021 aaagtagcgg taataaagat cttctttggt gactcgcgaa atgaattgct gaagttgtgg
     4081 ctcatcttct ggcaaaatcg ggcggaacaa gcagcgttca ccgtttttca attctaccca
     4141 ttcttccagc tgatgcggat aagggcgcac tgccagccga ctctcgttat cgtcttcaaa
     4201 cggcgcgata tccagcgtga catccagcgc ggtaaattca ctgccagaag ccagcaaagg
     4261 atgaatatcc agacgctgaa tttccgggca atcgacaatc aagttggaaa cctgcaccag
     4321 aagctggctc aagcctgcaa catccaatgg gcgtagcgca ctgcgcgcac gaatcttttt
     4381 acttttgatc ccctgaataa ccagatagcg ggccaggttc atattcagcg gcggcagtgc
     4441 gacgacggct tgatcttcag gacgccactc cacaccgcct tcacccagca tgatcaacgg
     4501 cccaaaaacc ggatcgtgct caaccacaac ccgcaactcc tgagcgccag cacggttagc
     4561 catactttgc accaacaggc cgtggacccg cgcctgcggc caggtcattt ttacgcgatc
     4621 gaaaatagcg tttgccgctt gctggacttc attggctgta cgcaggtaca gcatgacgcc
     4681 ctgaacttcc gatttatgtg gaatatccgg cgaacgcaat ttcagcgcca ccggataacc
     4741 aatctgttcg gcaatatgca ccgcttcggt gctatcacta gcaatccagg tagggagcgt
     4801 gttcatgcca tacgcttgca ggatgggctg aacttcatgg gtatcgagcg acgtagcccc
     4861 ttcggcaatc gcctgttgca acagaagatg cgcttttgcg gtattggaag tcagattgct
     4921 gggcaacgcc ggcgtttcgc gtagttgctt ctgattacgc cggtactcca ccatatgcat
     4981 aaaagcagtg atggttcctt ccggggtacg gtaggtcggc agcccggctt cgctgaataa
     5041 acgtcgtgcc tcttgcgagg agtgctcgcc gcaccagttc gtcagcagag aaacatactt
     5101 gctgcgggga tgatgcttta ccgcttcaat taatgcttgc gcactttctg ttgcgggagc
     5161 agcggcgctg ggcgaatgaa taaccatcag cgcatcaaaa tcctggctat ggagcagaat
     5221 gtccagcgtt ttgacataat gctcgctgct ggcgtcatcg cgcagatcga gcgggttaga
     5281 gattgccaca tgtcctggca gtgcatcgcg cagtttctgg caggtttctt cgcttagtgt
     5341 tgccagcttg ccattgcgtg accataaggc atccagcgcc agcgcggcag gcgcagcacc
     5401 gttgctgata atcatcaacc ggtcgccacg cagcgggcgc atatggctaa gggtttctac
     5461 cgccgaaaac agttcgtggg tgtcctgtac ccgcaacaaa ccggcacgct gaatagccgc
     5521 atcccatgcc gggtccattc ctgccgtcgt gttgagcagt cgctgtgccg ccgggctacg
     5581 tccgctttta atcaccagaa tcggtttatt acgcgaggca ctacgggccg ccgaaacaaa
     5641 gcgtcgcgcg tcgcttaact gttcgagata gagcaggatg gcgctggttt tactgtcgcg
     5701 tgccagatag tcaagcaatt catcaacgtc gatatccagg ctgtcgccga gcgcaataaa
     5761 gtaggaaaag cccatctcac gctgttgcgc ccagtcgagg atggtgttgg agacggcagc
     5821 cgattgtgaa ataaacgcca gcttgccgcg tttaatcggc acaggcgaaa agctggcatt
     5881 cagaccttgc cagggagcca gtaaacccag actgtttggt ccaagcaggc gcatgttatg
     5941 gcgcagggcg caggcgcgga gatcttcgtg ttgcgatgcc ggggcggaaa gaataatgca
     6001 ggttttacag cctttctcgc cgagctcttc cagaagagca agattacggc tggcattggt
     6061 acataaaacc gcaaggtcgg gtgtaaaggg caagctggca atatccggcc aggccaacac
     6121 acccaacacc gctttccagg ctggcgtcac cgggagtacc ggtccgttaa agcctcccgc
     6181 cagcaggtta cgcatcatca ggtaacctgc gcgattgggt ttcatcgacg cgccaattac
     6241 cgctatcgat tttggtcgca gtagtgcttc cagtcctcgc tgactcatac cggtctcccg
     6301 cagaagtgac cgaatgattt taaacgcttt ctaactgttc tgctgtgatg ctacccagat
     6361 gttgcgtttt tcctgccaga tagcgtgttt taaagcgggt aaaatgctcg cctaaccctg
     6421 ctgccgcccc ggtatcgccg gccatatcta acagtgcgat ggctacttcg gcagtacaat
     6481 attggccttc agcctgggct tcacgcaggc gataggcaga aagccgggaa agatcgacgg
     6541 aaatgacggg aagattatcc agatacggac ttttacgaaa catcttgcga gcttccggcc
     6601 aggtaccatc gagcatgata aacagcggtg gcttaccggc aggtggtgtg aagatcactt
     6661 cccgttgctc atcagcatac gaggcgggaa agaccaccat tggctgataa tccgggtttt
     6721 gtaccagatc cagcaaatcc tgcgagggtt cggtacgcga ccattgaaac gcaacggtat
     6781 caggcaaaat atcagcaatc agacgcccgg tattactggg cttcattggc tcggtgtcga
     6841 acatcagcaa acagaagcga ctttttgctt gtgctggggt aattgtcgaa cagagacata
     6901 atttctctgg caaaagacag cgttggcagc gacgaacgcg attaccgcgg gcaagaaaag
     6961 gacgtgttgc gcgcgcaata cgctcggcac gtaactggag aacagcgttt tcggtcataa
     7021 gagagcgtcg aaaaaacgcc attgtcgcag aggagaaaac ggggcacaag atgcgccccg
     7081 gtaagattaa agagattcgt tcagccagct atcgaacggc gctttcggta ctgcgccatt
     7141 aagcatgtcg acaacctgac cgtttttgaa aatcatgatc gtcggtatac tacgaattcc
     7201 aaagcgactg ctcaattcac gttcagcttc ggtattcact ttcacaaagc gcactttacc
     7261 gctacgctct tgcgcgacat cttcaaaaat tggtgcgaaa ttacggcagg ggccgcacca
     7321 cggtgcccag aagtcgatca ccactggtag atcatctttc agcaatttgt cgagcgtttc
     7381 accggtcgca ttaatcacct ctccgtcaaa caagtcgtga ccgcagcgtc cgcattttgc
     7441 cgcatcttcg atccgatcgt cgggaatgcg attgatggcc tgacaatggg tacaaacggt
     7501 attcataact aacctcggga tgagtaagga gacttatcgg caacgacgcc ggtatgtttc
     7561 taatatgtta catattatcg gggacattgt tccagacaac aatctgtttt attcaatagg
     7621 tatgatagtc gcaggctttg gtacgaaatc atggcgaacc gcgcggactt cgggtaatct
     7681 gcgcgcttcg cgcagcgctg gtggagaaaa gcatgaacga tgaaatgaaa ggtaaaagcg
     7741 gcaaggtcaa agtgatgtat gtccgcagtg atgatgattc tgataaacgt acccacaacc
     7801 cgcgtaccgg gaaagggggc gggcgtccag gaaaatctcg tgctgacggt ggccgtcgcc
     7861 ccgcccgcga tgacaaacag agtcagcccc gtgaccgcaa gtgggaagag tcgccgtggc
     7921 gcacggtttc ccgcgcgccg ggtgatgaga cgccggaaaa ggccgatcac ggtggcatca
     7981 gtggtaaaag ttttattgat ccagaagttt tgcgtcgtca gcgtgcggaa gaaacccgcg
     8041 tctacggcga aaatgcatgt caggcactgt tccagagccg tccggaagcg attgttcgcg
     8101 cctggtttat ccagagcgta acgccacgtt ttaaagaagc cttgcgctgg atggcggcaa
     8161 accgcaaagc gtaccatgtg gtggatgaag cggaattgac aaaagcgtca ggcacggaac
     8221 atcacggtgg cgtttgcttc ctgatcaaaa agcgtaatgg cacaaccgtg cagcagtggg
     8281 taagccaggc aggtgcgcag gattgcgttc tggcactgga aaacgaatct aacccgcata
     8341 acctgggtgg catgatgcgc agctgcgcgc acttcggcgt gaaaggcgtt gtggtgcagg
     8401 atgcggcact gctggagtcg ggtgcggcta tccgtaccgc agaaggcggc gcagagcacg
     8461 ttcagccgat tactggcgac aacattgtta acgtgctgga tgatttccgt caggcgggtt
     8521 acaccgtagt gacaacttcc agcgagcagg gtaagccgct gttcaaaacc agtctgccag
     8581 cgaaaatggt actggtgctg ggtcaggaat atgaagggtt accggatgcc gcacgcgatc
     8641 cgaacgatct gcgcgtgaag attgacggta cgggcaacgt tgccgggctg aatatctctg
     8701 ttgcaacagg cgttttgctt ggtgaatggt ggcgtcagaa taaagcctga taaatcagcc
     8761 gggtgacaac tctgccatcc ggcattttcc cgcaaattta ctcactctct gccggtaata
     8821 ctggcatcca gtcaatcggc gtctcgccac gttgttccag ccactgattt gccagcacaa
     8881 aatggttaca gccaaagaat ccacgatgcg cagaaagcgg tgacggatgc ggtgctttca
     8941 gtacgtgatg gcgttgctta tctataatcg cccctttctt ttgcgcatgc gatccccaca
     9001 acaaaaacac cacgccttcg cgatgctggt taatcaggct gatcacttta tcggtgaagg
     9061 tttcccagcc gaggctggcg tgggaatgcg cctgacctgc gcgtaccgtc aacacagtat
     9121 tgagtagcag aacgccctga cgcgcccagc tttcaagata accatgatta gggcgggtga
     9181 agcccggaat agtattttcc agctctttat acatattcag cagtgacgga ggagtggcga
     9241 ttccggggcg aacggaaaat gccagcccat gcgcctgtcc cggtccgtga taaggatcct
     9301 ggccgagaat caccacttta acgtcaccca actcggtaaa gcggaacgcg ttaaagacat
     9361 ctttttgtgg tggatagata gtgacgccgg actgccgctc gctggcgacg gtctgaaggg
     9421 tattaagaaa atagggttgc tgcttctctt cagccagcac gtcatgccag gttaattcgt
     9481 tagccatctc gctctcctgc gaatcttcaa tccgcctagc ttaactgctt ctttctacag
     9541 aacaaaatca tgctcatttt ttagacttaa acgagtgcaa aaaaatgtaa attggtaaaa
     9601 tttcacaccc gcataagttg atgtaaaaca ataaaatcca tttgcgacca cacttgatgt
     9661 gtggttttta ttgatttaaa tcaaagaatc aagggtgttt gaggagtata tatacactca
     9721 agcaacaatg gttttaccaa ttggccgcga caggctgaac aaatcaaata attttgccgg
     9781 ggaggcatca catgattaca ggtatccaga ttactaaagc cgctaacgac gatctgctga
     9841 actctttctg gctgctggac agcgaaaaag gcgaagcgcg ttgcatcgtt gcgaaagcag
     9901 gttatgcaga agacgaagtg gttgcagtaa gcaaactggg tgacattgag taccgtgaag
     9961 ttccagtaga agtgaaacca gaagttcgcg ttgaaggtgg tcagcacctg aacgttaacg
    10021 ttctgcgtcg tgaaactctg gaagatgcag ttaagcatcc ggaaaaatat ccgcagctga
    10081 ccatccgtgt atccggttat gcagttcgct ttaactctct gactccggaa cagcagcgcg
    10141 acgttatcgc tcgtaccttt actgaaagcc tgtaaggctt aagtagcaag ttactaagcg
    10201 gaagagggcg acctcttccg ctttttttca ttaatagaaa atgcgtaccg cgcaatagat
    10261 cagcagcagc gccagcacga tatttaactg gcgaccatac tggcgaaaca atcgctgaaa
    10321 cagatgcccc gccagcgccc agcacacatt accaaacgtc ccaatcattg ccagcaaaac
    10381 gctgacgcca actatccagc ttaacgcctg tgtttgcggc agaacaaatg tcgacagtgc
    10441 cgtaacaccg tacaaaatga ttttgacgtt cacaaactgc aaagcaaagc tggcccaaaa
    10501 actgattggt tttgtctgaa gcccgtcttc ctttgttgga ctggtagcga ttttccacgc
    10561 cagccagaca atatatgccg cccccgccca actcaacaga tgcacagctg ccgggtcgat
    10621 aacagccagt gaaaacgaaa tgcccgcaca cagcaacatg acgattaaaa atcccagact
    10681 catccctgcc agcacacggg tactctgacg aaatccgtgc gttgtggctg agctaagggc
    10741 gagaatattg ttcggtcctg gcgtcatggc ggtaatcagg gtgtaagtcc aaaaagcact
    10801 taaaagggtc ggtgtcactt gttatctcct ctgttttggt agacagtatc cgacacggaa
    10861 tatttaaaaa aatgaatgtt tttacattct aaattcgata ttttcgatag gtttggggtt
    10921 atggatctgc gccgttttat tacgcttaaa accgtggtgg aagagggttc ctttttgcga
    10981 gcttcgcaaa aattgtgctg cacacaatcg acggtgactt ttcatattca gcagcttgag
    11041 caggagttct cagtccagtt atttgagaaa attggccgac ggatgtgcct cactcgcgaa
    11101 ggtaaaaagc tgctgccgca catttatgaa ctcaccagag tgatggatac ccttcgtgag
    11161 gctgccaaaa aggagtcgga tccggacggg gaactacgcg ttgtttcggg cgaaacgttg
    11221 ctctcttacc gaatgccgca ggtgttgcag cgttttcggc aacgtgcccc aaaagtgcgt
    11281 ttatcgttgc agtcgctgaa ctgttacgtg atccgtgatg cgctactgaa tgatgaggcc
    11341 gatgttggcg tcttttatcg tgtagggaat gatgatgctc tgaatcgacg agagttgggt
    11401 gaacaatcac tggtgctggt ggcttcaccg caaattgcag atgtcgattt tactgagccg
    11461 ggaagacata acggctgtag ctttattatc aacgaaccgc aatgtgtctt ccggcagata
    11521 tttgagagca cgctgcgcca gcggcggatc acggtggaaa acaccattga gcttataagt
    11581 atcgaaagca tcaagcgttg tgtcgcggcg aatatcggag tcagctacct gccgcgtttt
    11641 gcggtggcaa aagagttgga gtgcggagaa ttaattgaat tgccttttgg cgaacagtcg
    11701 cagaccatta cggccatgtg cgcacaccat gctggaaaag cggttagccc ggcgatgcac
    11761 acttttattc agtgtgttga agagtatttt gtggttgctt aaaaaaacaa tgccgggctt
    11821 aaacccggca ttgagattac tcttctgtcg tttgcggtgg cacgcccgtt ccgcttggtt
    11881 tacggcgctt accaatattt ttggtatcgc gatggcgttt tttcacccgc ggcttctctt
    11941 tctctttagc ttttttcttc tcggcgcgtt ttgccagtac tttcttcgat ggcttgccgg
    12001 tctgcttctc gcttggtgca cgcgttttcg ggcgtaactc atcaattacg cgagctttaa
    12061 ttggctcttc aatatagcga ccaactttgc ccagcagcag atggtcatgg gcttccacca
    12121 gcgaaattgc ggtgccttta cgaccggcgc gcgcggtacg tccgatacgg tgcaaataag
    12181 tatcgccact gcgcggcata tcgaagttaa agacgtggct gacgtcagga atgtcgatac
    12241 cgcgcgcggc aacatcggtt gcaaccagta cgtttacgcg accttcggtc aaacgtttga
    12301 tcgcttcgtt acgcttgccc tgtaccatct caccttcgag atagcagttg ttgatgcccg
    12361 cttcgcgcag ccagtttgcc agctcatgca cacgctcacg cttacgcaca aacacaattg
    12421 agcgggtcgc ttccggctgt tttaacagat gcaccagcaa tgcggtttta tgttccaggt
    12481 catcggcgcg gtaataccac tgatgaattt ttttgcgctc acgggtggac ggattggcag
    12541 aaacctccac cggatcttcc agcagacgct cggcaaagtc ctgaatcgca tcgccttcca
    12601 gagttgccga aaagagcagg gtctgtttac gccagcgcgt ttcgccagca atatgttcga
    12661 tatcctgagc gaagcccata tccagcatac ggtctgcttc gtcgaggatc agcgtttcaa
    12721 ccgcgcggca atcgaagttc tcttctttta tgtattgcag cagacgtccg gtcgtggcga
    12781 ccacgatgtc ctgattttcg ctgaacactt ccgcgtggtt catataggct acgccgccgg
    12841 tgatggtggc gatatccaga tgcgtatgtt tcgccagttc gcgggcatga tcggccacct
    12901 gcatcgccag ctcgcgagtt ggggtgagga tcaaaatacg cggcggaccg gatttcttac
    12961 gcgggaaatc gagcaggtgc tgcaacgctg gcagcagata cgccgccgtt ttaccggtgc
    13021 ctgtcggcgc agaaccgagt acatcacggc catcgagcgc aggcggaatg gcggcagcct
    13081 gaatggcggt cgggcgagtg aaacctttat cctggagggc ttccagcagg ctttcgtcga
    13141 gttcaagttc ggaaaaagtc gttacagtca tgttctacct ctgtgtgggg cgctgattat
    13201 agacgttacg gctgcaatct tcatctgttt gtatggatat cgcttctcgg gtattgttct
    13261 cacccggtgc tatcgccgtt tttcctgcaa ggttttattt tcatgtcaca gtctacatcc
    13321 gtgcttcgtc gtaatggatt tacttttaaa cagttttttg ttgctcacga tcgctgtgcg
    13381 atgaaagtgg gaacggatgg catattgctg ggcgcatggg caccggtggc tgggataaaa
    13441 cgttgccttg atatcggtgc gggtagtggc ttgctggcac tgatgctggc gcagcgaacc
    13501 agtgacagcg tgataattga tgccgttgag ctggaaagtg aagctgcctc tcaggcgcag
    13561 gaaaatgtcg cccagtcccc gtggttagag agaattaacg tccatacggc ggatattcag
    13621 cagtgggtaa cacagcagac cgcgcgcttt gatttaatca tcagtaaccc accttactat
    13681 cagcagggag tagagtgctc gacacctcaa cgggaacagg ctcgctatac tactacgctt
    13741 gatcacccat cgttgctggc ctgtgcggcg gagtgcatta ccgaagaggg atttttctgc
    13801 gtggtgctgc cagagcagat tggtaatagt tttacggagc tggcattaag tatgggctgg
    13861 catttacgtt tgcggacgga tgtggcggaa aacgaagcgc gactgccgca tcgggtgctg
    13921 ctggcattct ccccgcaggc gggagaatgc tttagcgatc gcttagtcat tcgtgggcca
    13981 gaccagaact attccgaagc gtatacggcg ctgacccagg ctttttatct gtttatgtaa
    14041 tgattgccgg gagaaaggat cgatggaccg gaatgcgtga gcagttccgg gtaatccagc
    14101 gtaaaatgca ggccacgact ctccttgcgc atcatcgcac agcgaacaat caactcggca
    14161 acctgtacca gattacgcag ctccagcaaa ttatttgaga cgcggaaatg ggcgtaatat
    14221 tcgtctattt cttgttggag cattgttatc cgccgcaggg cgcgttccag gcgcttcgtt
    14281 gtgcgcacaa tgccaacgta atcccacata aacagccgta gctcgtgcca gttatgctga
    14341 attactaccc gttcgtcagg gttctcaacg cggctttcat cccacggcgg taacgtactg
    14401 acgccgtggg cataaggcat acgtctggtg atatcttctg ccgccgacca gccatagacc
    14461 agacactcca gcaatgaatt cgaggccatg cggttagcgc cgtgtaagcc ggtataactc
    14521 acctcgccaa tggcatacaa gccctcgacg tccgtccgcc catgatcatc aaccattaca
    14581 ccaccgcagg tataatgtgc agcaggcaca atcggtaccg gttcttgtgt gagatcaatc
    14641 cccagcccga gcaatttttc ataaatcatc gggaaatgct ggcgaataaa atcggcgggc
    14701 ttatggctga tatcaaggaa catacaatcc gcgccgaggc gtttcatttc atggtcaatg
    14761 gcgcgggcga caatatcgcg cggggccagt tcgccgcgca catcaaaatc gggcataaaa
    14821 cgcgtgccat ccgggcgctt gagataagcg ccttcgccgc gcagtgcttc cgttaacagg
    14881 aaattgcgtg cctgtgggtg atacagcgcg gtagggtgga actgattaaa ttcgagattg
    14941 gcaacccggc agcctgcgcg ccacgccata gcaatgccat cgccagaaga aatatccgga
    15001 ttggtggtgt actgataaac cttcgacgca ccgccggttg ccagcaccac cgcttttgcg
    15061 tggcaggttt ccaccttttc tttatttcgg ttccataccc acgcgccaac aacccgtcgc
    15121 gtgcccggca ggccaatttt gtcagaaata atcagatcaa ccgcgttgct gcgctccagc
    15181 acgcgaatat tcggatggtt cagcgccttg ctcaccagcg tggtttctac ttctctacca
    15241 gttgcgtcgg cggcatgaag aatacgacgg tgactatgtc caccttcacg ggtcagatgg
    15301 tagctttctt cgccattcgg ctgaatgtgg gtatcaaata acaccccctg gtcgattagc
    15361 cattgcacac aggatcgtgc attgctggcg acaaattcaa ctgcatggcg atcgcaaata
    15421 ccagccccgg caatcaatgt gtcttccaca tgcgagtcaa tgctgtcagt ttcatcaaac
    15481 acggcggcaa taccgccctg ggcataaaat gttgaacctt ccgttaccgg gcctttactt
    15541 agaacgatga cctgatgctg gtcagccagg cgtagcgcca gtgaaagccc ggctgcgccg
    15601 ctaccgataa tcaacacgtc acatgaatgt tcagggagag tattcatttt ctttgtttaa
    15661 tttactaaac acggtttggt cagcatagca tcatgttgtg cgaataaaca cctgctattt
    15721 taatatttgt tacagtggct aaacacgctg acgcagggcg gcgagaaaaa gagaagttac
    15781 tggctggtgg aggattaggt ggtgaaataa aaaggccgtt gggttactct tcaggcagtt
    15841 aaatgggcat ttctacacag ataatgcgat gttcagattc tgtagactta taatgataga
    15901 taatgatccg tctacagcat gacaaacaaa aacagatgcg ttacggaact ttacaaaaac
    15961 gagacactct aaccctttgc ttgctcaaat tgcagctaat ggagtggcgt ttcgatagcg
    16021 cgtggaaatt tggtttgggg agactttacc tcggatgagc gagcagttaa cggaccaggt
    16081 cctggttgaa cgggtccaga agggagatca gaaagccttt aacttactgg tagtgcgcta
    16141 tcagcataaa gtggcgagtc tggtttcccg ctatgtgccg tcgggtgatg ttcccgatgt
    16201 ggtacaagaa gcttttatta aagcctatcg tgcgctggat tcgttccggg gagatagcgc
    16261 tttttataca tggctgtatc ggattgctgt aaatacagcg aaaaattacc tggttgctca
    16321 ggggcgtcgt ccaccttcca gtgatgtgga tgccattgaa gctgaaaact tcgaaagtgg
    16381 cggcgcgttg aaagaaattt cgaaccctga gaacttaatg ttgtcagaag aactgagaca
    16441 gatagttttc cgaactattg agtccctccc ggaagattta cgcatggcaa taaccttgcg
    16501 ggagctggat ggcctgagct atgaagagat agccgctatc atggattgtc cggtaggtac
    16561 ggtgcgttca cgtatcttcc gagcgaggga agctattgat aacaaagttc aaccgcttat
    16621 caggcgttga cgatagcggg atactggata agggtattag gcatgcagaa agaacaactt
    16681 tccgctttaa tggatggcga aacgctggat agtgagctgc ttaacgaact ggctcataac
    16741 ccagaaatgc agaaaacctg ggaaagctat cacttaatcc gtgactcaat gcggggtgat
    16801 actcccgagg tgctccattt cgatatctct tcacgcgtga tggccgccat tgaagaagag
    16861 ccagtacgtc aaccggcgac attgatcccg gaagcccagc ctgcgccgca tcaatggcag
    16921 aaaatgccat tctggcagaa agtacgtccg tgggcggcac agcttaccca aatgggcgta
    16981 gcagcatgcg tatcgcttgc agttatcgtt ggcgtccagc actataatgg acaatctgaa
    17041 acgtcccagc agcccgaaac gccggtattt aatacactgc cgatgatggg taaagccagc
    17101 ccggtaagcc tgggagtacc ttctgaagcg accgcaaaca atggtcaaca gcagcaggta
    17161 caggagcagc gtcgtcgcat taatgcaatg ttgcaggatt acgaactgca acgccgactc
    17221 cactctgaac agcttcagtt tgagcaggca caaacccagc aagccgctgt acaggtgcca
    17281 ggaattcaaa ctttaggaac gcaatcgcag taatgaagca actttggttt gccatgtcat
    17341 tagtgacagg tagcctgtta ttctctgcta acgcctcggc cactcccgcg tccggggcgt
    17401 tattacagca gatgaacctg gctagccagt cactgaatta cgagctgtca ttcatcagca
    17461 tcaataaaca aggtgttgag tctctgcgtt atcgacatgc acgcctcgat aaccgtccgc
    17521 ttgcacagtt gttgcaaatg gatggcccgc gccgggaagt ggtacagcgc ggcaatgaaa
    17581 tcagctactt tgagccgggc cttgaaccgt ttactcttaa tggcgattac attgttgatt
    17641 ctctgccatc gctcatctat accgatttca aacgcctttc tccttactac gactttatct
    17701 ccgtcgggcg cacgcgtatt gctgatcgtc tttgtgaagt tattcgcgtg gttgctcgtg
    17761 atggcacacg ctacagctac attgtgtgga tggacaccga atcgaaatta ccgatgcggg
    17821 ttgacctcct tgatcgcgat ggtgaaacgc tggaacaatt tcgcgtgatt gcttttaatg
    17881 tcaatcagga tatcagcagc agtatgcaga cgctggcgaa ggcaaatttg ccgccgttgc
    17941 tttctgttcc tgtaggtgaa aaagccaaat tcagctggac tcccacctgg ttgccacagg
    18001 gttttagcga agtttccagt agtcgacgtc cgctaccgac gatggacaac atgcctatcg
    18061 aatcacgtct ctattccgac ggattattca gcttctcggt aaacgttaac cgcgctacgc
    18121 catcgagcac cgatcagatg ttgcgcaccg gacgcagaac cgtcagtaca agcgtacgtg
    18181 ataacgccga aatcaccatt gtcggtgaac tgccgccgca aacggcgaaa cgcattgccg
    18241 agaatattaa gttcggggca gcgcaatgat caaagagtgg gctaccgtcg tctcctggca
    18301 aaacgggcag gcgctggtca gttgtgatgt taaagcctca tgcagcagct gcgcttcacg
    18361 cgccggttgc ggtagccgcg tgttgaataa acttggcccg caaaccacgc ataccattgt
    18421 cgtaccctgt gatgagccgt tagtgccggg gcaaaaagtg gaattgggga tcgccgaagg
    18481 cagcctgctt agctccgcat tactggttta tatgtcgccg ctggtgggat tattcctcat
    18541 cgcttcgcta tttcagctac tctttgcttc tgacgtcgca gcattatgtg gtgcggtcct
    18601 cggcggtatt ggcgggttcc tgattgcgcg tggctactcg cgtaagtttg ctgcccgggc
    18661 agaatggcaa ccgatcatct taagcgtggc attgccgcct ggccttgtgc gatttgaaac
    18721 atcttcggaa gacgcgagcc agtgatttca ctggctttgt tgcattttgc ataacaaatg
    18781 cgaggacgtt tccagatacc acaatccata cggtatgaac atttcctcgc ctcaatgttg
    18841 tagtgtagaa tgcggcgttt ctattaatac agacgttaag ctcagaacag cgacttctaa
    18901 aagcctggtt aaccgggcat taaggcacaa taatcatact ttatgaagaa tatacgtaac
    18961 ttttcgatca tagctcacat tgaccacggt aaatcgacgc tgtctgaccg tattatccag
    19021 atctgcggtg gcctgtctga ccgtgaaatg gaggcgcagg ttctcgattc catggatctt
    19081 gagcgtgagc gtggcattac catcaaagcg caaagcgtga cgctggacta caaagcgtct
    19141 gacggcgaaa cctatcagct taactttatc gacaccccgg gccacgtaga cttctcctat
    19201 gaagtttccc gttcgctggc tgcctgtgaa ggtgcattgc tggtggtcga cgccgggcag
    19261 ggcgtggaag cgcaaaccct ggcaaactgc tacaccgcca tggaaatgga tctcgaagtg
    19321 gtgccggtac tgaacaagat tgacctgccg gcagccgatc ctgaacgcgt ggcggaagaa
    19381 attgaagata tcgtcggcat cgacgccact gacgcggtgc gctgttcagc gaaaaccggc
    19441 gttggcgtgc aggacgttct cgaacgtctg gtgcgcgaca ttccgccgcc ggaaggcgat
    19501 ccggaaggcc cgttgcaggc actaattatc gactcatggt tcgacaacta cctgggcgtt
    19561 gtttcactta tccgtattaa aaacggcacc ctgcgtaagg gcgacaaagt gaaagtcatg
    19621 agtaccgggc agacctataa cgccgaccgt ctgggcatct tcacgccgaa acaggttgac
    19681 cgcactgaac tgaaatgtgg cgaagtaggc tggctcgtat gtgcgattaa agatatccac
    19741 ggcgctccag tcggcgatac cttaacgctg gcgcgtaatc cggcagaaaa ggcgctgcct
    19801 ggctttaaaa aagtcaaacc gcaggtatac gccggtctgt tcccggtaag ttccgacgac
    19861 tatgaggcct tccgtgacgc gctgggtaaa ctcagcctga acgatgcctc actgttctat
    19921 gagccggaaa gctcaagcgc gctgggcttt ggtttccgct gcggcttcct tggcctgctg
    19981 cacatggaga tcatccagga gcgtctggaa cgtgaatacg atctggatct gatcaccact
    20041 gcgccgaccg tagtgtatga agttgaaacc acgtcaaggg aagttatcta cgtcgacagc
    20101 ccatccaagc tgcctgcggt aaataacatt tacgaactgc gcgagccgat tgcagagtgt
    20161 cacatgctgc tgccgcaggc atatctcggc aacgttatta cgctgtgcgt agaaaaacgc
    20221 ggcgtgcaga ccaatatggt ttaccacggt aatcaggtgg cgctgacgta cgagatcccg
    20281 atggcggaag tggtgctcga cttcttcgat cgcctgaaat ctacctcgcg tggttatgcg
    20341 tctctggatt acaacttcaa acgcttccag gcgtccgaca tggtacgtgt agacgtatta
    20401 atcaacggtg aacgtgttga tgcgctggcg ttgatcaccc accgtgataa ttcgcaaaac
    20461 cgcggtcgcg agttggtgga gaagatgaaa gatctgatcc cacgccagca gtttgatatc
    20521 gccattcagg cagcgattgg tacgcacatt attgcgcgat ccaccgtgaa acagctgcgt
    20581 aaaaacgtac tggctaaatg ttatggcggc gatatcagcc gtaagaaaaa gctgctgcag
    20641 aagcagaaag aaggtaagaa acgcatgaag cagatcggta acgtcgagct gccgcaggaa
    20701 gcgttcctcg ccattctgca cgtcggcaaa gacaacaaat aacccttagg agttggcatg
    20761 gcgaatatgt ttgccctgat tctggtgatt gccacactgg tgacgggcat tttatggtgc
    20821 gtggataaat tctttttcgc acctaaacgg cgggaacgtc aggcagcggc gcaggcggct
    20881 gccggtgact cactggataa agcaacgttg aaaaaggttg cgccgaagcc tggctggctg
    20941 gaaaccggtg cttctgtttt tccggtactg gctatcgtat tgattgtgcg ttcgtttatt
    21001 tatgaaccgt tccagattcc gtcaggttcg atgatgccga ctctgttaat cggtgatttt
    21061 attctggtag agaagtttgc ttatggcatt aaagatccta tctaccagaa aacgctgatc
    21121 gaaaccggtc atccgaaacg cggcgatatc gtggtcttta aatatccgga agatccaaag
    21181 cttgattaca tcaagcgcgc ggtgggttta ccgggcgata aagtcactta cgatccggtc
    21241 tcaaaagagc tgacgattca accgggatgc agttccggcc aggcgtgtga aaacgcgctg
    21301 ccggtcacct actcaaacgt ggaaccgagc gatttcgttc agaccttctc acgccgtaat
    21361 ggtggggaag cgaccagcgg attctttgaa gtgccgaaaa acgaaaccaa agaaaatgga
    21421 attcgtcttt ccgagcgtaa agagacactg ggtgatgtga cgcaccgcat tctgacagtg
    21481 ccgattgcgc aggatcaggt ggggatgtat taccagcagc cagggcaaca actggcaacc
    21541 tggattgttc ctccgggaca atacttcatg atgggcgaca accgcgacaa cagcgcggac
    21601 agccgttact ggggctttgt gcctgaagcg aatctggtcg gtcgggcaac ggctatctgg
    21661 atgagcttcg ataagcaaga aggcgaatgg ccgacaggtg tgcgcttaag tcgcattggc
    21721 ggcatccatt aatagctcat cttcgttcac gttgtcgccg ttatggcgac aacgtgaatt
    21781 atttatgaga taaatctccc gtggctaacg acatcccccg tcgttgtgta tagaatattc
    21841 ccccgaagtt taaggttggc acctccaggt tgccacggca cacgaaacag cgttggtccc
    21901 catataccgc taaactgaaa ctgcagcgaa gcagttagca gagccatgta tatcaggtct
    21961 gtttcgtgtg ctgaattgtt gacgcattta tttattggta tcgcatgaac cccatcgtaa
    22021 ttaatcggct tcaacggaag ctgggctaca cttttaatca tcaggaactg ttgcagcagg
    22081 cattaactca tcgtagtgcc agcagcaaac ataacgagcg tttagaattt ttaggcgact
    22141 ctattctgag ctacgttatc gccaatgcgc tttatcaccg tttccctcgt gtggatgaag
    22201 gcgatatgag ccggatgcgc gccacgctgg tccgtggcaa tacgctggcg gaactggcgc
    22261 gcgaatttga gttaggcgag tgcttacgtt taggaccagg tgaacttaaa agcggtggat
    22321 ttcgtcgtga gtcaattctc gccgacaccg tcgaagcatt aattggtggc gtattcctcg
    22381 acagtgatat tcaaaccgtc gagaaattaa tcctcaactg gtatcaaact cgtctggacg
    22441 aaattagccc aggcgataaa caaaaagatc cgaaaacgcg cttgcaagaa tatttgcagg
    22501 gtcgtcatct gccgctgccg acttatctgg tagtccaggt acgtggcgaa gcgcacgatc
    22561 aggaatttac aatccactgc caggtcagcg gcctgagtga accggtggtt ggcacaggtt
    22621 caagccgtcg taaggctgag caggctgccg ccgaacaggc gttgaaaaaa ctggagctgg
    22681 aatgagcatc gataaaagtt actgcggatt tattgccatc gtcggacgtc cgaacgttgg
    22741 caaatccaca ttgttgaata aattgctggg gcagaaaatc tccatcacct cccgcaaggc
    22801 gcagacaact cgtcaccgca ttgtggggat ccatactgaa ggcgcgtatc aggcgatcta
    22861 cgtcgataca ccgggcctgc atatggaaga aaaacgcgcc attaaccgcc tgatgaacaa
    22921 agcggcgagc agctctattg gcgatgttga gctggtgatt tttgtcgttg aaggcacccg
    22981 ctggacgccg gacgacgaaa tggtgctcaa caaactgcgc gaaggcaaag cgccggtaat
    23041 cctcgcggtg aacaaagtgg acaacgtgca ggagaaagcc gatctgctgc cgcacctgca
    23101 gttcctggca agccagatga acttcctcga tatcgtgcca atctctgccg aaaccgggct
    23161 gaatgttgac actattgcgg caatcgtgcg taagcatcta cctgaagcga ctcatcactt
    23221 cccggaagat tacatcaccg atcgctcaca gcgttttatg gcgtctgaaa tcatccgcga
    23281 aaaactgatg cgtttcctcg gcgctgaact gccgtactcc gtgaccgtgg agatcgaacg
    23341 tttcgtctct aacgaacgcg gtggttatga catcaacggt ttgattctcg ttgagcgtga
    23401 agggcagaag aagatggtca ttggcaacaa aggggccaag atcaaaacca tcgggattga
    23461 agcgcgtaaa gacatgcagg aaatgtttga agcgcctgtt caccttgagc tgtgggtaaa
    23521 agtgaaatcc ggttgggccg acgacgaacg cgcactgcgc agtctcggtt acgttgacga
    23581 tctttaagag taactccgat ggaaggctgg cagcgcgcat ttgtcctgca tagtcgcccg
    23641 tggagcgaaa ccagcctgat gctggacgtc ttcacggagg aatcgggtcg cgtgcgtctg
    23701 gttgcgaaag gcgcacgctc taaacgctct accctgaaag gtgcattaca gcctttcacc
    23761 cctctcttgc tacgtttcgg cgggcgtggc gaagtcaaaa cgctgcgcag tgctgaagct
    23821 gtctcgctgg cgctgccatt aagcggtatc acgctttaca gcggtctgta catcaacgaa
    23881 cttctctccc gcgtactgga atacgagacg cgcttctctg aactcttttt cgattacttg
    23941 cactgcattc agtctcttgc agggggcact ggtacgccag aacccgcgct gcgccgcttt
    24001 gaactggcac tgctcgggca tctgggttat ggcgtcaatt ttacccattg tgcgggtagc
    24061 ggcgagccgg tagatgacac catgacgtat cgttatcgcg aagaaaaagg gtttatcgca
    24121 agcgtcgtta tcgacaataa aacgttcacc ggaaggcagt taaaagcgtt aaacgcacgg
    24181 gaatttcctg acgcagacac actgcgcgcc gcgaaacgct ttacccgcat ggcgcttaag
    24241 ccgtatcttg gcggtaaacc tttaaagagc agggaactgt tccggcaatt tatgcctaag
    24301 cgaacggtga aaacacatta tgaatgatga ggattgtcat ggctgaatta ctgttaggcg
    24361 tcaacattga ccatatcgct acgctgcgca acgcgcgcgg taccgcttac ccggatccgg
    24421 tgcaggccgc gtttattgcc gagcaggcgg gggcggatgg cattaccgtg catttacgtg
    24481 aagatcgccg ccacattact gaccgcgatg tgcgcatcct gcgtcagacg ctggataccc
    24541 gcatgaatct ggagatggcg gtgaccgaag agatgctggc gatcgcggtt gagacgaagc
    24601 cgcatttttg ctgcctggta ccagaaaagc gtcaggaagt aacaaccgaa ggcggcctgg
    24661 acgttgcagg gcagcgtgac aaaatgcgcg atgcctgcaa acgtctggcg gatgccggga
    24721 ttcaggtttc tctgtttatc gacgccgatg aagagcagat caaagctgcg gcagaggttg
    24781 gcgcaccgtt tatcgagatc cacaccggtt gctatgctga tgccaaaact gacgccgaac
    24841 aggcgcaaga gctggcgcgt atcgccaaag ccgcgacctt tgccgctagt ctcggtttga
    24901 aagttaacgc cggacacggt ctgacctatc acaacgtaaa agccattgcc gccatccctg
    24961 agatgcacga actgaatatc ggtcatgcca ttattggtcg tgcagtgatg accggactga
    25021 aagatgcggt ggcagaaatg aagcgcctga tgctggaagc gcgtggctaa tggcaatatt
    25081 aggtttaggc acggatatcg tagagatcgc tcgcatagaa gcagtgatcg cccgatccgg
    25141 tgagcgcctg gcacgccgcg tattaagcga taacgaatgg gaaatttgga aaacgcacca
    25201 ccagccggtg cgttttctgg cgaaacgctt tgccgtaaaa gaagccgcag caaaagcgtt
    25261 tggcaccggg atccgcaatg gtctggcgtt taatcagttt gaagtattta acgatgagct
    25321 tggtaagcca cggctgcgac tgtggggcga ggcattaaaa ctggcggaaa agctgggcgt
    25381 tgcaaatatg catgtgacgc tggccgatga gcgccactat gcttgcgcca cggtaattat
    25441 tgaaagttaa aaataatacc gttctcttta aaaagtaaaa cccccgaact ggtaaggaac
    25501 gggggtttta ctttgtaact ttaatttagt gcagcacgca ccctcaacaa cagcgattat
    25561 ggatcagggt agttatcttt tacaagcaac ttgcaatctt tatcataaaa actcgagcct
    25621 ttacgaagaa agcgatattg atggcaagat taacgtgacc gccaattcgt aagtacatta
    25681 aaattggttt cgttattggc gattttgctg tgctttacac catgccacag aattccccca
    25741 ttgaaacgag tggtgtcgtc aaagctctgg tgtggagtgc agcatgcacc ctcaataact
    25801 cgcacgttca gttttgggga gatgtaaggg ctaatctgaa cggctgcatt ccttgtttaa
    25861 ggaaaaacga atgactgatt gccgatacct tattaaacgg gccatcaaaa tcatcattgc
    25921 tgttttacag ctgatccttc tgttcttata acacaaggaa acgtacttaa ggtgcgtccg
    25981 gtgaaccagt cggacgcacc tttaataact ata
//
LOCUS       DAZUCE010000050        26012 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZUCE010000050 DAZUCE010000000
VERSION     DAZUCE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.050"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(220..2793)
                     /gene="clpB"
                     /locus_tag="ACHY03_001757"
     CDS             complement(220..2793)
                     /gene="clpB"
                     /locus_tag="ACHY03_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFS3904997.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2923..3654)
                     /gene="yfiH"
                     /locus_tag="ACHY03_001758"
     CDS             complement(2923..3654)
                     /gene="yfiH"
                     /locus_tag="ACHY03_001758"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFS3904998.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3651..4631)
                     /gene="rluD"
                     /locus_tag="ACHY03_001759"
     CDS             complement(3651..4631)
                     /gene="rluD"
                     /locus_tag="ACHY03_001759"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFS3904999.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKLCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEAMRADFEEHKDEVDWL"
     gene            4766..5503
                     /gene="bamD"
                     /locus_tag="ACHY03_001760"
     CDS             4766..5503
                     /gene="bamD"
                     /locus_tag="ACHY03_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFS3905000.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5774..6115
                     /gene="raiA"
                     /locus_tag="ACHY03_001761"
     CDS             5774..6115
                     /gene="raiA"
                     /locus_tag="ACHY03_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFS3905001.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6218..6343
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6219..6266
                     /gene="pheL"
                     /locus_tag="ACHY03_001762"
     CDS             6219..6266
                     /gene="pheL"
                     /locus_tag="ACHY03_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFS3905002.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6365..7525
                     /gene="pheA"
                     /locus_tag="ACHY03_001763"
     CDS             6365..7525
                     /gene="pheA"
                     /locus_tag="ACHY03_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFS3905003.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7568..8689)
                     /gene="tyrA"
                     /locus_tag="ACHY03_001764"
     CDS             complement(7568..8689)
                     /gene="tyrA"
                     /locus_tag="ACHY03_001764"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFS3905004.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8700..9770)
                     /gene="aroF"
                     /locus_tag="ACHY03_001765"
     CDS             complement(8700..9770)
                     /gene="aroF"
                     /locus_tag="ACHY03_001765"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFS3905005.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9980..10345
                     /gene="yfiL"
                     /locus_tag="ACHY03_001766"
     CDS             9980..10345
                     /gene="yfiL"
                     /locus_tag="ACHY03_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFS3905006.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYTRGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10495..11013
                     /gene="yfiR"
                     /locus_tag="ACHY03_001767"
     CDS             10495..11013
                     /gene="yfiR"
                     /locus_tag="ACHY03_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFS3905007.1"
                     /translation="MRFSHRLFLLLIILLTGAPILAQEPSDVAKNVRMMVSGIISYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISDCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11003..12229
                     /gene="dgcN"
                     /locus_tag="ACHY03_001768"
     CDS             11003..12229
                     /gene="dgcN"
                     /locus_tag="ACHY03_001768"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFS3905008.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISNFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNRPFDLHNGHLTTMTLSIGYAMTIAHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12245..12727
                     /gene="yfiB"
                     /locus_tag="ACHY03_001769"
     CDS             12245..12727
                     /gene="yfiB"
                     /locus_tag="ACHY03_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3905009.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAKGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12804..13151)
                     /gene="rplS"
                     /locus_tag="ACHY03_001770"
     CDS             complement(12804..13151)
                     /gene="rplS"
                     /locus_tag="ACHY03_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFS3905010.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13193..13960)
                     /gene="trmD"
                     /locus_tag="ACHY03_001771"
     CDS             complement(13193..13960)
                     /gene="trmD"
                     /locus_tag="ACHY03_001771"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFS3905011.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHCTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13991..14539)
                     /gene="rimM"
                     /locus_tag="ACHY03_001772"
     CDS             complement(13991..14539)
                     /gene="rimM"
                     /locus_tag="ACHY03_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFS3905012.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14558..14806)
                     /gene="rpsP"
                     /locus_tag="ACHY03_001773"
     CDS             complement(14558..14806)
                     /gene="rpsP"
                     /locus_tag="ACHY03_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFS3905013.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14943..16304)
                     /gene="ffh"
                     /locus_tag="ACHY03_001774"
     CDS             complement(14943..16304)
                     /gene="ffh"
                     /locus_tag="ACHY03_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFS3905014.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16471..17262
                     /gene="ypjD"
                     /locus_tag="ACHY03_001775"
     CDS             16471..17262
                     /gene="ypjD"
                     /locus_tag="ACHY03_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFS3905015.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17284..18570
                     /gene="yfjD"
                     /locus_tag="ACHY03_001776"
     CDS             17284..18570
                     /gene="yfjD"
                     /locus_tag="ACHY03_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFS3905016.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18625..19218)
                     /gene="grpE"
                     /locus_tag="ACHY03_001777"
     CDS             complement(18625..19218)
                     /gene="grpE"
                     /locus_tag="ACHY03_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFS3905017.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPDASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19341..20219
                     /gene="nadK"
                     /locus_tag="ACHY03_001778"
     CDS             19341..20219
                     /gene="nadK"
                     /locus_tag="ACHY03_001778"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFS3905018.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20305..21966
                     /gene="recN"
                     /locus_tag="ACHY03_001779"
     CDS             20305..21966
                     /gene="recN"
                     /locus_tag="ACHY03_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFS3905019.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22115..22456
                     /gene="bamE"
                     /locus_tag="ACHY03_001780"
     CDS             22115..22456
                     /gene="bamE"
                     /locus_tag="ACHY03_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFS3905020.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22518..22808)
                     /gene="yfjF"
                     /locus_tag="ACHY03_001781"
     CDS             complement(22518..22808)
                     /gene="yfjF"
                     /locus_tag="ACHY03_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFS3905021.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22798..23235)
                     /gene="ratA"
                     /locus_tag="ACHY03_001782"
     CDS             complement(22798..23235)
                     /gene="ratA"
                     /locus_tag="ACHY03_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFS3905022.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23406..23888
                     /gene="smpB"
                     /locus_tag="ACHY03_001783"
     CDS             23406..23888
                     /gene="smpB"
                     /locus_tag="ACHY03_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFS3905023.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24103..24465
                     /gene="ssrA"
                     /locus_tag="ACHY03_001784"
     tmRNA           24103..24465
                     /gene="ssrA"
                     /locus_tag="ACHY03_001784"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24669..25910
                     /gene="intA"
                     /locus_tag="ACHY03_001785"
     CDS             24669..25910
                     /gene="intA"
                     /locus_tag="ACHY03_001785"
                     /inference="COORDINATES: protein motif:HMM:NF007246.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFS3905024.1"
                     /translation="MARKTKPLTDTEIKAAKPKDADYQLYDGDGLTLLIKSSGSKLWQ
                     FRYYRPLTKQRTKQSFGAYPAVSLSDARKLRAESRVLLAKDIDPQEHQKEQVRNSQEA
                     KTNTFLLVAERWWNVKKASVTEDYADDIWRSLERDVFPAIGDISVTEIKAHTLVKAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LPQLMQTMRTANISQSTRCLFMWQLLTITRPAEAAEARWDEIDFEAKEWKIPAARMKM
                     NRDHTVPLSDGALAILAMMKSLSGGREFIFPSRIKPTQPMNSQTVNAALKRAGLGGVL
                     VSHGLRSIASTALNEQGFPPDVIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANL
                     IKAADSIPTVCNEVAGLRLVG"
BASE COUNT         6636 a         6838 c         6131 g         6407 t
ORIGIN      
        1 ttctctgcca gaattctcag gagaaccccg ctgacccggc ggcgtgtttg ccgttgttcc
       61 gtgtcagtgg tggcgcatta tagggagtta ttccggcctg acaagcgaaa aatataaaaa
      121 ctttatcgtt cgctcacttt tcaggcaaaa catcttaaat atagtctttt ccgtctaact
      181 tatagacaaa aacgagcccc gaagggctcg ttttatcatt tactggacgg cgacaatccg
      241 gtcttcatta acttccaggc gaatcacttt acccggaacc aattcaccag acagtatttg
      301 ctgtgccagc gggttttcga tctgctgctg aattgcacgt ttcagaggac gtgcaccata
      361 gaccggatcg taaccgttct cgctcagcag tttcagcgcc tcgtcagaaa tgtggatttc
      421 ataaccacgt tcttccagac gtttatacag acgtttcaac tgaatctgcg caatcgacgc
      481 aatgtgctgt tcacccagcg gatggaagac caccacttca tcgatacggt taatgaattc
      541 cggacggaag ttatggctaa ccacaccgag caccagctct ttcatgtgcg cataatccag
      601 ttcaccgaag cgttcctgaa tcaaatcgga accgaggtta gaggtcataa tgacaaccgt
      661 attacggaag tcgaccgttc tcccctgccc gtcagtcaga cgcccatcat ccagtacctg
      721 caacagaatg ttgaagacat ccggatgcgc tttttccact tcatccagca ggataacgga
      781 atacggacga cgacgcacag cttcggtcag gtagccacct tcttcataac caacatatcc
      841 cggaggcgca ccaaccagac gagacaccga gtgtttctcc ataaactcgg acatatcgat
      901 acggaccatc gcctcgtcgc tatcaaacat aaagttcgcc agcgccttac aaagctctgt
      961 tttccccaca ccagttgggc cgaggaacag gaatgaacca atcgggcgat ttggatccgc
     1021 cagccccgca cggctacgac gaatagcgtt agataccgca tcaaccgctt cgttctgacc
     1081 aattacgcga tggtgcagtt cttgctccat acgcagcagt ttttcgcgct cgctttccat
     1141 catgcgagaa accggaatcc ccgtccaacg cgccagcact tcagcaattt cggcgtcggt
     1201 cactttatta cgcaacagac gcatagtttt gccttcgagc tgcgttgcgg cttccagttg
     1261 cttttccagt tccgggattt tgccgtattg cagttcagac atccgcgcca ggtcccccac
     1321 acggcgagcc tgttcaatag cgattttcgc ctgttccagt tccgctttaa tggtctgcgt
     1381 accagaaagc gatgccttct ctgctttcca ctcttcttct aactcggagt actgacgttc
     1441 tttgtcgctc agttcttcgt tgagcatatc caggcgtttt ttactggctt catcagactc
     1501 tttcattaac gcctgttgtt ccagtttgag ctggatgata cggcgatcga gtcggtcgag
     1561 ttcttctggt tttgagtcaa tctgcatacg aatgctggat gctgcttcat cgatcaggtc
     1621 gatggcttta tccggcagct gacggtcagc aatgtagcga tgagacaacg tcgccgctgc
     1681 aacaattgcc gggtcagtaa tttgcacatg gtggtgcaat tcgtaacgtt ctttcaggcc
     1741 acgtagaatc gcaatggtat cttcaacaga aggctcggca acaaacactt tctggaaacg
     1801 acgttccagc gcagcatctt tttcaatgta ctggcgatat tcgtcaagcg tcgtggcacc
     1861 tacgcagtgc aattcaccac gcgccagcgc cggtttcagc atgtttccgg cgtccattgc
     1921 gccatcggct ttacccgcgc cgaccatggt atgtaattcg tcgataaaca ggatgacgtt
     1981 gccttcctgt ttggcaagat cgttaagcac gccttttaaa cgttcttcaa actcaccgcg
     2041 atatttcgcc ccagccacca gcgcgcccat atccagcgcc agtacccggc ggcctttcaa
     2101 cccttccggc acttcgccgt tgataatacg ctgcgccaga ccttcaacga tggcagtttt
     2161 accgacgccg ggttcaccaa tcagtaccgg gttattttta gtacgacgtt gcagcacctg
     2221 aatggtacgg cgaatttctt catcacgacc aatcaccgga tcgagtttgc cctgttcggc
     2281 tcgttcggta aggtcgatgg tatatttttt caaagcctga cgttggtctt cagcaccttg
     2341 atcgttcacg ctttcacctc cacgcatttg ttcaatcgct tgagtaatgt tggcggtggt
     2401 cgcccctgct gctttcagga tgtcggccag cgtgccgcga gactcaagtg ccgccagaac
     2461 gaacagttct gacgagataa agttatcacc acgtttttgc gccagcttgt cgcaaagatt
     2521 aagaacgcgc accagatcct gtgatggctg gacatcacca ccagtacctt caacctgcgg
     2581 taaacgattt aatgcctgat tgatatctgt gcgcaactgg ccagcattta tgccagctga
     2641 tgttaacaaa ggactaaccg accccccttc ctgattcagc agggcgctca ttaaatgaag
     2701 tggttcgatg aattggttgt cgtgcccgag tgcaagtgat tgggcatcgg caagagcaag
     2761 ctggaattta ttagtaagac gatccagacg cataactcct cccataacgg atcaaagttg
     2821 ctactggaga ttaaatgagg tcatccctca attattcaag gttattgacc agattaatgt
     2881 gaaaagaaaa tcacgcgtac cggatcgtct tgattcttta ggttatatca gccaaatgaa
     2941 acttgccata cgcccggtgg tcttgtcgcg acgataagag aagaaagtct cattttccgt
     3001 atatgtacaa cggtcgccgc cgaaaatttg ttcaacacca acgctcgcca gacgctgccg
     3061 ggcaagcaga taaatatccg ccagatactt atcaccatgc tgaatgaaag ctgtactcgc
     3121 tttggcgtct gctgccataa acgcctcgcg aacctccgcc cccacttcga acgcgcgtgg
     3181 accaattgcc ggccctaacc aggcgagaat attttccgga ttatcagcaa aacaggaaac
     3241 tgtctcttcc agcacgcctg cgcacagtcc acgccagcca gcatgagcgg cggcgacttc
     3301 cgttcccgct cgattgcaaa acagcacagg gaggcagtcg gcagtcatca ctgcgcaaac
     3361 cgtgccaggc gtattgctat aagaggcatc tgctcgtttt gaggcataag gttcgccagt
     3421 aagcttaagc acatctttgc cgtgtacctg ctcaagccag accggtttag aaggcaaatt
     3481 gcccgcagca aaaagtcgct tgcgattctc ctcaacgtga tccgggttat cgccacaatg
     3541 ggcaccgagg ttgagcgagt catacggggg caagctcacg ccgccgatac gagtagagct
     3601 acaagccgca acaccttttg gcagcggcca ctgcgggaca atcagcttac tcataaccag
     3661 tccacttcat ccttatgttc ttcgaaatcg gcacgcatcg cctcaatcag ctccaccata
     3721 tcttgtggaa taggcgcatg ccattccatt tcgatgccgg agatcgggtg ataaagacgc
     3781 agcatggttg catgaagcgc ctggcggtca aacttacgca gcgtggagat aaacgcttcc
     3841 gaagcacctt ttggcggacg cggacggcca ccataaaccg gatcgcccac cagcggatga
     3901 gtgatatggg ccatatgcac gcggatctgg tgcgtacgtc cagtttccag acgcaaccgc
     3961 agacgcgtgt gcacacggaa gtgttccatg atgcgatagt gagtcaccgc tggtttgccc
     4021 atcggatgca ccgccatgtg agtacgtttg gtcgggtggc gactgattgg ttcatccacc
     4081 gtaccgccag cggtcatatg accaatcgcc accgcttcgt actcacgagt aatttcacgc
     4141 ctttgcaaag attcgactaa acgcgtctga gccggaacgg tttttgccac aaccatcagg
     4201 ccagtggtgt ctttatccag acgatggacg atgcccgcac gcggtacatc ggcaataggc
     4261 ggataataat gaagcaacgc attcagtacc gtgccatccg ggttacccgc gccaggatga
     4321 accaccaggt cgcgcggttt attaatgacg atgatgtctt catcttcata gacgatatcc
     4381 agcgggatat cctgcggttc aaaacgcgct tcttcttcaa tctctgcgtt gatggcaacc
     4441 tgctcgccac ccaatacttt ttctttcggc ttatcacaaa gtttgccgtt aaccagcacg
     4501 cgctggtcga ggatccattc ttttattcgc gaacgtgaat aatccgggaa catttcggcc
     4561 aaagcctgat ctaagcgttg accgagttgg ttttcggaca ccgttgcagt gagctgtact
     4621 cgttgtgcca tatatactgc ttcttcgttt atcgttgggg ttttacggct ttgccgttta
     4681 atatagtgtg ctattgtagc tggtcttaac cgggagcagg aacagagaat ctcccgtatt
     4741 acattttgag gaaagtcaaa acgtcatgac gcgcatgaaa tatctggtgg cagccgccac
     4801 actaagcctg tttttggcgg gttgctcggg gtcaaaggaa gaagtacctg ataatccgcc
     4861 aaatgaaatt tacgcgactg cacaacaaaa gctgcaggac ggtaactgga gacaggcaat
     4921 aacgcaactg gaagcgttag ataatcgcta tccgtttggt ccgtattcgc agcaggtgca
     4981 gctggatctc atctacgcct actataaaaa cgccgatttg ccgttagcgc aggctgccat
     5041 cgatcgtttt attcgcctta acccgaccca tccgaatatc gattatgtca tgtacatgcg
     5101 tggcctgacc aatatggcgc tggatgacag tgcgctgcaa gggttctttg gcgttgaccg
     5161 tagcgatcgc gatcctcaac atgcacgagc tgcgtttagt gacttttcca aactggtgcg
     5221 cggctatcca aacagtcagt acaccaccga tgccaccaaa cgtctggtat tcctgaaaga
     5281 tcgtctggcg aaatatgaat actccgtggc cgagtactat acagaacgtg gcgcatgggt
     5341 tgccgtcgtt aaccgcgtag aaggcatgtt gcgcgactac ccggataccc aggctacgcg
     5401 tgatgcgctg ccgctgatgg aaaatgcata ccgtcagatg cagatgaatg cgcaagctga
     5461 aaaagtagcg aaaatcatcg ccgcaaacag cagcaataca taacagaaac ctgaaacaca
     5521 aaacggcagc ccttgagctg ccgttttttt attctgtcag ttgtgaaact gaagcgattt
     5581 agtcactatc gatctcatca aatatggctc gctttgagat attcctcaag taaaaaaaca
     5641 cctcttcctg cgatttctca caaaaaaact tcgttgacaa aaagtgacaa aattatgaga
     5701 ttttcatcac acattttgac atcaggaacg gtatgctgaa ttcaccaaga cgggaagaca
     5761 agaggtaaaa tttatgacaa tgaacattac cagcaaacaa atggaaatta ctccggccat
     5821 ccgccaacat gtcgcagacc gtctcgccaa actggaaaaa tggcaaacac atctgattaa
     5881 tccacatatc attctgtcca aagagccaca agggtttgtt gctgacgcca caatcaatac
     5941 acctaacggc gttctggttg ccagtggtaa acatgaagat atgtacaccg caattaacga
     6001 attgatcaac aagctggaac ggcagctcaa taaactgcag cacaaaggcg aagcacgtcg
     6061 tgccgcaaca tcggtgaaag acgccaactt cgtcgaagaa gttgaagaag agtagtcctt
     6121 tatattgagt gtatcgccaa cgcgccttcg ggcgcgtttt ttgttgacag cgtgaaaaca
     6181 gtacgggtac tgtactaaag tcacttaagg aaacaaacat gaaacacaca ccgtttttct
     6241 tcgcattctt ttttaccttc ccctgaatgg gaggcgtttc gtcgtgtgaa acagaatgcg
     6301 aagacgaaca ataaggcctc ccaaatcggg gggccttttt tattgataac aaaaaggcaa
     6361 cactatgaca tcggaaaacc cgttactggc gctgcgagag aaaatcagcg cgctggatga
     6421 aaaattatta gcattactgg cagagcggcg cgaactggcc gtcgaggtgg gaaaagccaa
     6481 actgctctcg catcgcccgg tacgtgatat tgatcgtgaa cgcgatttac tggaaagatt
     6541 aattacgctc ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct
     6601 catcattgaa gattccgtat taactcagca ggctttactc caacaacatc tcaataaaat
     6661 taatccgcac tcagcacgca tcgcttttct cggccccaaa ggttcttatt cccatcttgc
     6721 ggcgcgccag tatgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt
     6781 tgccgatatt tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg taccgattga
     6841 aaataccagc tccggtgcca taaacgacgt ttacgatctg ctgcaacata ccagcttgtc
     6901 gattgttggc gagatgacgt taactatcga ccattgtttg ttggtctccg gcactactga
     6961 tttatccacc atcaatacgg tctacagcca tccgcagcca ttccagcaat gcagcaaatt
     7021 ccttaatcgt tatccgcact ggaagattga atataccgaa agtacgtctg cggcaatgga
     7081 aaaggttgca caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac
     7141 tttgtacggt ttgcaggtac tggagcggat tgaagcgaat cagcgacaaa acttcacccg
     7201 atttgtggtg ttggcgcgta aagccattaa cgtgtctgat caggttccgg cgaaaacgac
     7261 gttgttaatg gcgaccgggc aacaagccgg tgcgctggtt gaagcgttgc tggtactgcg
     7321 caaccacaat ctgattatga cccgtctgga atcacgcccg attcacggta atccatggga
     7381 agagatgttt tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt
     7441 gaaagagtta ggggaaatca cccgttcaat gaaggtattg ggctgttacc caagtgagaa
     7501 cgtagtgcct gttgatccaa cctgatgaaa aggtgccgga tgatgtgaat catccggcac
     7561 tggattatta ctggcgattg tcattcgcct gacgcaataa cacgcggctt tcactctgaa
     7621 aacgctgtgc gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct
     7681 gcttatcgcc ctgctccagc aactcaatcg cctcgccgaa acgcttatag taacgtttga
     7741 ttaacgccag attacgctct gacgacataa tgatgtcggc ataaagctgc ggatcctgag
     7801 caaacagtcg cccgaccatc gccagctcaa ggcggtaaat cggcgaagag agcgccagaa
     7861 gttgctcaag ctgaacattt tcttctgcca ggtgcagccc gtaagcaaaa gtagcaaagt
     7921 ggcgcagagc ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca
     7981 accgagcgcc ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc
     8041 catcacacca gaccacaact tgctttgcca ggctaccgct gtccgggccg aacatcgggt
     8101 gtaaccccag taccggacca tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca
     8161 ctgatgccag atcaaccaga atacaatctt tcggtaaagg cggtaatttg ccgataactt
     8221 gctcagtaac gtggattggc acactaacaa tcaccattcc ggcatcggca acaatatcag
     8281 ccgctcgatc ccagtcatgt tgctctagaa tccgcacctg ataacccgat agtgtcagca
     8341 tcttctcgaa caggcgcccc atctgaccgc caccgccgac gataaccacc ggacgcagtg
     8401 acggacaaag tgttttaaat cctttgtcgt tttcactgga gtaagattct cgcatcaccc
     8461 gacgcaaaac atcctcaatc agatctggcg gtacacccag agcttccgcc tctgcacgac
     8521 gcgaggccaa catagatgcc tcgcgctccg gaacataaat aggcagtcca aagcggcttt
     8581 tcacctcgcc cacttcagca accagttcca gacgcttcgc taataaattc agcagcgctt
     8641 tatcgacttc atcaatttga tcgcgtaatg cggtcaattc agcaaccata ataaacctct
     8701 taagccacgc gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca
     8761 tcggtcattt cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg
     8821 cgcggttgct cggaagactg attgccctcg tggatattac tttcgatcat cagaccaata
     8881 attgagcgat tgccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg
     8941 cgataatctt tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc
     9001 gcctgttcca tctctttttc acattgcgca acatccgcag ggctatagtt cggcgcttta
     9061 ccaccgcgca ggatgacatg cccgtccgga ttcccctgag tttgtagcaa cgcaacctgc
     9121 cctgcctggt taatgccaac aaaacggtgc ggctgggcgg cggcgcgcat agcgttaatt
     9181 gctgttgcca gactgccgtc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggat
     9241 gccatttcac ggtgagtttg cgattccgtt gtacgagcac caattgctga ccagctaaac
     9301 agatcgccca ggtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc
     9361 atattcacca gctcaagcag caatttacgc gcgatctgca gaccggcttc tacatcaaaa
     9421 gagccatcca tatggggatc gttaattaac cctttccagc cgacagtggt acggggtttt
     9481 tcaaaataga cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta
     9541 aatcgacgag catattccag agcagtttcc ggatcatgaa tggaacaagg accacatact
     9601 accagcagac gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca
     9661 atctgggctt cttgttgcag gcttaatgga aaagcggcct tcagttgtgc cggagtcatt
     9721 aaaacctgtt cgtcggtaat atgtacgtta ttcagcgcgt ctttttgcat gatggcgatc
     9781 ctgtttatgc tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata
     9841 aagtaaagtt ttcaatccat atttcgtaca tttatcttta cataagcaat ttattcgcgc
     9901 tttcaaccct tgattctgta tagataaatt tacaccacct ttgaaaacaa tccgctatgc
     9961 tttgaaaaag gagaaagaaa tgatgaaaaa gtttatcgcc cccttgttgg ctttactggt
    10021 tagcggatgc cagattgatc cttatactca cgcgccaacc ttgaccagca ccgactggta
    10081 tgatgttggt atggaagatg cgatatcggg cagcgccata aaagatgacg atgcatttag
    10141 cgattcacag gcggatcgcg gtctatacct taaaggatat gccgaaggac aaaagaaaac
    10201 ttgccagacc gattttactt atacccgagg actttccggt aaaagctttc ctgccagctg
    10261 caacaatgtt gaaaatgcca gccaactgca tgaagtatgg caaaaagggg ctgatgaaaa
    10321 tgccagcgca atacgattaa attaattaaa accccacaaa taaattaaac ataagatttt
    10381 actcattatg ttttatttct cacaatgatg acgaccgcat gaaataatga tattttaatg
    10441 agcattctga aaaaatatcg atgcatttcg agcgaagatg gtggggatcc ctgaatgcgt
    10501 ttttctcacc gactgttctt actgctgatt atcctgctga cgggagcacc aatactcgct
    10561 caggaacctt ctgacgttgc gaaaaacgtg cgtatgatgg tgtccggtat tatcagttac
    10621 acccgctggc ctgcgctatc agggccacca aagttatgta tcttttcatc ttcacgtttt
    10681 agtaccgccc tacaagagaa tgccgcgaca tctttgcctt atctgcctgt catcattcac
    10741 actcaacaag aggcgatgat ttcagactgc aatggttttt attttggcaa cgagtcaccg
    10801 acattccaga tggaattaac cgaacaatat ccgtcaaagg cgttattatt aattgccgaa
    10861 caaaataccg agtgcattat tggtagcgcc ttttgtctaa tcatccacaa taatgacgtc
    10921 agatttgccg taaacctgga tgccttatcg cgtagcggtg taaaggtcaa cccggatgtc
    10981 ttgatgctcg cacggaagaa aaatgatgga taacgataat tctcttaata agcgccccac
    11041 gtttaaaaga gcattacgca acatcagtat gaccagcata tttatcacta tgatgctgat
    11101 ctggttgctg ctttccgtga cctcggtgct gaccctgaaa cagtacgcgc aaaaaaacct
    11161 ggcactgaca gcagcaacaa tgacttacag tctggaagca gctgtcgttt ttgccgatgg
    11221 ccctgcagca actgaaacac tggcagcgct gggccagcaa gggcaatttt caactgcaga
    11281 agtacgtgat aagcagcaaa atattctggc atcctggcat tacacccgta aggatccagg
    11341 cgatactttc agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat
    11401 tcgtcacaat ggcgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag
    11461 caattttatc tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggaat
    11521 cgcaattacc ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac
    11581 cgatgtcgta catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg
    11641 tatcgctgag tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga
    11701 gtggcagctt cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc
    11761 attaaccggg ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa
    11821 ttccgatgcc cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat
    11881 caatgatacc tggggtcatg cgacgggcga tagagtcttg attgaaatcg caaaacggtt
    11941 agctgaattt ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat
    12001 ggtgctctat gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca
    12061 aatctttaat cgcccgtttg atcttcataa tggccatctg accactatga cattaagcat
    12121 tggttacgcg atgaccattg cgcacgcctc tgcggaaaaa ttacaagagc ttgccgatca
    12181 caatatgtat caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat
    12241 cgatatgata aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg
    12301 ccagtcccct cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt
    12361 tactgaatcc gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa
    12421 tgactacaaa ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc
    12481 ctcgacaggg ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagatag
    12541 ttacaacgaa ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctaaggg
    12601 tggacaaatt ccgcgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc
    12661 cagtaacaag accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattactac
    12721 cccttaaaac acgagcaaac agataaaaaa agggccagcc aattggccag cccttcttaa
    12781 caggatgtcg cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca
    12841 gtacgctcac gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa
    12901 atgctgtcaa ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa
    12961 attttacgaa cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc
    13021 tcgaatgcct gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta
    13081 tcacccggac ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata
    13141 atgttgctca taatttaatc tcttatcctg ggtaaactga tatctcgggg gcttacgcca
    13201 tcccatcatg tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg
    13261 cttgctcttc agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc
    13321 ccagcgactg tttcaaacgc cagcgacgta tctcggcatg gtttcccgac agtaacactg
    13381 gcggaacttc catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc
    13441 cttcagcaaa ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc
    13501 gggaaacgga gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc
    13561 cgattgacca ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc
    13621 gaccgcacac cagaatcaat ttttgattcg ttgctagctc gctgacgccc gcttgatcaa
    13681 gcttgcgtcc ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg
    13741 ctgcatgaat ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt
    13801 aaggacgatc gtccacggta cagtgccggt catgcgtgaa gtcgcgagga ctccagctct
    13861 ggatgctcag caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc
    13921 ggaacatttc aggaaacagg ctaattatgc caatccacat agcgccgtct tttaccgttt
    13981 atccggtggt ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga
    14041 ctttcttgat aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat
    14101 ctttcaggtt tgccttaatg acgagaacgt cattggatcc ggtttccatc atatcgacga
    14161 ctttaccgag atcgtagcct tcagtggtta ctacctggca gcccataagg tctttccagt
    14221 agtagtcccc ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg
    14281 tcagcaggtt cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat
    14341 tgtggtgctt ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc
    14401 agggctgata gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc
    14461 cacgaatacc gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg
    14521 cggtgagttg tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt
    14581 tgatcagcgc agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc
    14641 gatccagatc caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa
    14701 cgcgctcgat gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga
    14761 acggacgctt tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg
    14821 tgaataaaac aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa
    14881 aattttactg atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc
    14941 atttagcgac caggaaatcc cggcggcatc atccccttca tgcttctcat catcttcgcc
    15001 attccgccct tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg tttcagaaga
    15061 cggttaacgt cctgcacctg cataccgcaa ccggcagcaa tacggcgttt acgcgaacct
    15121 ttgatgattt ctggcttagc gcgctctttc atcgtcatcg agttgatgat ggcttccata
    15181 cgcaccagca ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc
    15241 ggcagcttgc ccatcaggct agccatgccg cccatatttt tcatctggcg cagctgctca
    15301 agaaagtcgt tgagatcgaa gccgtcacct tttttcagct tgctggctaa tttctctgcc
    15361 tgcgcgcggt caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg
    15421 agaatacgcg acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca
    15481 acaccgagga acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg
    15541 cgggcatcgc cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat
    15601 gcttttgccg tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca
    15661 accgggttaa tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc
    15721 agacgaccag cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc
    15781 gccgcgttaa caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc
    15841 acctgctctg ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa
    15901 accaccagca ctttcttctt gtgcttctcg cgcaggaact taccgagttt accaacgctg
    15961 gttgttttac cggcaccttg caggcccgcc atcagtacga ccgcaggcgg ttgcgcagcc
    16021 aggttcaggg tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg
    16081 acgaactcct gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt
    16141 acgcgattga taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg
    16201 cgcacttcgc gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata
    16261 ttgcgcagcg tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg
    16321 tggaaacggt tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa
    16381 ggcgtctttg ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc
    16441 tttcttattg ctcaaactgt cgacatcact atgcccgttt ttgctctgct cgcgcttgtc
    16501 gcctactccg tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg
    16561 cggcgcatgg ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa
    16621 gcccgcatcc tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca
    16681 ttggtcagtt tgatgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg
    16741 ttgctgctac ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg
    16801 cccaatgaat acatcaccca tctggaagct acgcctggga tgctggtgca cattggctta
    16861 tcgctctttt cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg
    16921 attgattacc aactgaagaa caagaagctg gcgtttaacc aggaaatgcc gccattgatg
    16981 agtatcgagc gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg
    17041 ctttgcactg gcctgttcta catgcacaac ttatttagca tggaaaatat cgacaaggct
    17101 gtgctctcta tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttatcatgaa
    17161 ggatggcgtg gacgtcgcgt cgtctggttt aacgttgcgg gtgcagtcat tctgacactg
    17221 gcctacttcg gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc
    17281 cccctggaac acatttctac tactacgttg atcattattc tgatcatcat ggtggtcatt
    17341 tcagcctatt tttccgggtc cgaaaccgga atgatgaccc tcaaccgcta tcgtctgcga
    17401 catatggcga aacagggtaa tcgctcggcc aaacgcgtcg aaaaattgct gcgtaagcca
    17461 gaccgcctga taagcctggt gttaatcggc aataacctgg tcaatattct tgcctccgcg
    17521 ctcggcacta ttgttgggat gcgtttgtac ggcgatgcgg gcgtggcaat tgcgactggt
    17581 gtgctgactt ttgtcgtact ggtttttgct gaggtattgc cgaaaaccat tgccgcgctg
    17641 tacccggaaa aagtcgctta tccgagtagt tttctgctgg ctccgctgca aattttgatg
    17701 atgccgctgg tctggttgct gaatgctatc acccgtatgc tgatgcgcat gatgggtatc
    17761 aaaaccgata tcgtggttag cggctctttg agcaaagaag agttgcgcac tatcgtgcac
    17821 gaatcgcgct cacaaatttc ccgtcgcaat caggatatgc tgctgtcggt gctcgatctg
    17881 gaaaaaatga ccgttgatga catcatggtg ccgcgcagtg aaattatcgg tattgatatc
    17941 aacgatgact ggaaatctat tctgcgccaa ctctcccact cacctcacgg gcgcatcgtg
    18001 ctctaccgtg attcgctgga cgacgccatc agtatgctgc gagtacgtga agcctggcgg
    18061 ttgatgtcgg agaaaaaaga gttcaccaaa gaaaccatgc tgcgcgccgc ggacgagatc
    18121 tattttgtgc cggaaggtac gccgctcagc acgcagttgg tgaagtttca gcgcaacaaa
    18181 aagaaagtcg gcctggtcgt caacgagtat ggagacattc aggggctggt gacggttgaa
    18241 gatattctgg aagagattgt cggcgatttc actacgtcga tgtcgccaac acttgccgaa
    18301 gaagtcacgc cgcaaaacga cggttcggtg attattgatg gcactgccaa cgtgcgggaa
    18361 atcaacaaag cctttaactg gcatctaccg gaagatgatg cccgcacggt taatggcgtc
    18421 attcttgagg cactggaaga gatccctgtc gcaggcaccc gcgtgcgtat tggcgagtac
    18481 gatatagata ttctcgacgt acaggacaat atgattaagc aggtaaaagt ttttcctgtg
    18541 aaaccgctgc gcgagagtgt ggcggagtaa cgaaaacggc cctgcattcg catgcagggc
    18601 cgtgaattat tacgaaaaca gaaattacgc ttttgctttc gctacagtta ccatcgccgc
    18661 acgaatcgta cgaccattca gcgtataacc cttctgcata atgcccagta cgttacctgg
    18721 cgcaacgtca tcagattcca ccattgcgat agcctgatgc acattcgggt ccagcggcac
    18781 gttagtttcg gcgatcactt caacgccaaa cttacgcaca acatccagca tcgacttcag
    18841 cgtcagctca atgccttcaa ccatcgcaga catatccggg ttagctttat cagccacttc
    18901 cagcgcacga tccaggctat caatcaccgg cagcaattcg ttgatgaatt tctccagcgc
    18961 gaatttgtgg gctttttcaa tatccagttc agtacgacga cgcaggtttt ccatttcggc
    19021 ttttacacgc aaaatgccgt cacgttcacg ggtctgggct tcagccagct gagcttcgag
    19081 attcgcaatt ttttcatcgc gcggatccac ctgctcagca gaagcgtctg gctcaactgc
    19141 ctcaatctct tcgtgctgat ccatgataat ttcttccggg gcttgcccct caggcgtttt
    19201 ctgttcttta ctactcatga atttctccgc gtttttttcg cattcatctc gctaacttcg
    19261 cttattatgg ggatcagttt cagggtttca agggaagcac tcacattgtc atcaatcttc
    19321 gcaacaagga cctcggaaaa atgaataatc atttcaagtg tattggcatt gtgggacacc
    19381 cacggcaccc tactgcactg acaacacatg aaatgctcta ccgctggctg tgcacaaaag
    19441 gttacgaggt catcgttgag caacaaatcg ctcacgaatt gcaactgaag aatgtgaaaa
    19501 ctggcacgct cgcggagatt gggcaactgg ctgatctcgc ggtagtcgtt ggtggcgacg
    19561 gtaatatgct gggcgcggcg cgcacgctcg cccgttacga tattaaagtt attggaatca
    19621 accgtggcaa cctgggtttc ctgactgacc ttgaccccga taacgcccag caacaattag
    19681 ccgatgtgct ggaaggtcac tacatcagcg agaaacgttt tttgctggaa gcgcaagtct
    19741 gtcagcaaga ttgccaaaag cgcatcagca ccgcgataaa tgaagtggta cttcatccag
    19801 gcaaagtggc gcatatgata gagttcgaag tgtatatcga cgagatcttt gcgttttctc
    19861 agcgttctga tgggctaatt atttcgacgc caacaggctc caccgcctat tccctctctg
    19921 cgggcggtcc tattctgacc ccctctctgg atgcgattac cctggtgccc atgttcccgc
    19981 atacgttgtc agcacgacca ctggtcataa acagcagcag cacgatccgt ctgcgttttt
    20041 cgcatcgccg taacgacctg gaaatcagtt gcgacagcca gatagcacta ccgattcagg
    20101 aaggtgaaga tgtcctgatt cgtcgctgtg attaccatct gaatctgatt catccgaaag
    20161 attacagtta tttcaataca ttaagcacca agctcggctg gtcaaaaaaa ttattctaat
    20221 tttacgccag cctctttact gtatataaaa ccagtttata ctgtacacaa taacagtaat
    20281 ggtttttcat acaggaaaac gactatgttg gcacaactga ccatcagcaa ctttgctatc
    20341 gttcgtgagc ttgagattga ttttcatagc ggcatgaccg taataactgg cgagaccggc
    20401 gcgggtaaat ctattgcaat agatgccctc ggtctttgtc tcggtggtcg cgctgaagcc
    20461 gacatggtgc gtaccggcgc tgctcgcgct gacctgtgcg cccgtttttc tctgaaagat
    20521 acgccagcgg ccctgcgctg gctggaagaa aaccagcttg aagacgggca tgaatgtttg
    20581 cttcgtcgcg taatcagcag cgatggtcgc tctcgtggtt tcatcaacgg tacagctgtt
    20641 cctctgtcac aactgcgcga actgggtcag ttgctgattc agatccatgg tcagcacgct
    20701 catcagttac tcaccaaacc cgagcaccaa aagttcctgc ttgatggcta tgccaatgaa
    20761 acctctctac tccaggaaat gaccgcacgt tatcagttgt ggcatcaaag ctgccgtgac
    20821 ctcgcgcatc atcaacagtt aagtcaggaa cgcgctgccc gtgcggaact gctgcaatac
    20881 caattaaaag aacttaacga atttaatccg cagcccggag agtttgagca aatcgacgaa
    20941 gagtacaaac gtctggcgaa cagcggtcaa ttgctgacca ccagccagaa tgcattggca
    21001 ttaatggccg acggtgaaga cgcaaacctg caaagtcagc tttacacggc taaacaactg
    21061 gtgagcgaat tgattggcat ggacagcaaa ctgtccggcg tacttgatat gctggaagaa
    21121 gctaccatcc agattgctga agccagcgac gaactgcgcc actactgcga tcgtttggat
    21181 ctcgatccca accggctgtt tgaacttgaa caacgcatct caaaacagat ttcgctggca
    21241 cgtaaacatc acgtcagccc tgaggcatta ccacagtatt accagtcgct actggaagaa
    21301 cagcagcaac tggacgatca ggccgactca caagaaacgc tcgcgctggc ggtaacgaaa
    21361 catcatcagc aggcactgga aaccgcgcgc gcattacacc aacaacgcca gcaatatgca
    21421 gaagaacttg cacagctgat caccgacagt atgcatgcgc tctcaatgcc gcatgggcag
    21481 tttacgatcg atgttaaatt tgacgagcat cacctgggcg ctgacggtgc cgatcgtatt
    21541 gagtttcggg taaccaccaa cccaggtcag ccaatgcagc ctattgccaa agtcgcatcc
    21601 ggtggtgaat tgtcccgcat cgcactggca atccaggtca tcacggcgcg taaaatggaa
    21661 accccggcac tgatttttga tgaagtggat gtagggatta gcggtccaac agcggcagtt
    21721 gtcggcaaac tgctgcgtca actcggcgaa tcaactcagg tgatgtgtgt tacccacctg
    21781 ccacaagtcg cgggatgtgg tcatcaacac tattttgtca gcaaagaaac cgatggtgcg
    21841 atgacagaaa cgcatatgca atccctgaat aaaaaagcgc ggttacaaga gctggcgcgc
    21901 ctgcttggtg gcagtgaagt cacacgtaac acactggcga atgcgaaaga actgcttgca
    21961 gcgtaaactt ttttcctgct tcacggtcag agtaaacagc aaaacgccgt aagaccggaa
    22021 agcaaaaggt tttaaagtga tgaaaggtct attatcatcg gcatattaca gatgagccac
    22081 gtactgctcg ggcccgaaaa ggaatcaaat cactatgcgc tgtaaaacgc tgactgctgc
    22141 agcagcagta ctattgatgt tgaccgcagg ctgttccact ctggagcgag tggtttaccg
    22201 tcctgacatc aaccagggga actatctgac cgctaacgac gtatccaaaa tacgtgttgg
    22261 catgacgcaa caacaagttg cgtacgcatt gggtacaccg ctgatgtccg atccatttgg
    22321 tacgaatacc tggttctatg tcttccgcca gcaaccaggt catgaaggtg taacgcagca
    22381 aacgctgacg ctaaccttta acagtagcgg tgtgttgacc aatattgata acaaacctgc
    22441 gctgagtggt aactaataat aaagttgctc tcaaagacgt taaaaaaggt gctcaatgag
    22501 cacctttttt ctgtctgtta tttattcgct gatttttctg ctcgttgcct gcgcagctct
    22561 ttcggatcgg caatgagagg gcgataaatc tccacccgat cgccatcatg cacactatcg
    22621 ctcagttttg ccggacggct ataaatgccg actttgtttt tagttaaatc gatatcggtc
    22681 cgcaattcca gcaagccact ggcgcgaata gcttcttcaa ccgtcgcgcc ctcctgcagc
    22741 gtcacgcgct gcaggtactg cttctcaggt agcgcataag ccacctcaac ggcaatttta
    22801 cctggcactg taaacctctt tcgcacgaac cgtaaaagcc tggaccatat tagacgccag
    22861 ctctttaaac acgcgaccaa aggcgagctc aatcaactta ttggtaaact caaagtcgag
    22921 atgaaactcg atacgacacg cctcctggct cagcggcgta aacttccatc caccgatcaa
    22981 tttcttgaac ggcccatcca ccagattcat aagaatactt tggttactgg tcaactggtt
    23041 gcgggtagta aacgttttgc tgatcccagc cttagagaca tctaccgccg cagtcatctg
    23101 cccaggagtg gactccagaa tccgacttcc ggtacaaccc ggcaaaaact gaggataaga
    23161 ctgaacgtca ttcactaact gatacatttg ctccgcgctg tagggtacca gtgcggtccg
    23221 gctaatctga ggcataacaa tttccatcaa caaaaatcca acaaataata tcatttatcc
    23281 tgctgtaaaa aaaacgctat cccggcgctg ggtaacatcg ggttcatgct aagatagagc
    23341 cttgtccccc gcaggattga tatggggtgt tttcgatttc agattaccga tgattcacga
    23401 cgcttatgac gaagaaaaaa gcacataaac ctggttcagc gaccatcgcg cttaataagc
    23461 gcgcccgtca cgaatacttt atcgaagaag agttcgaagc gggacttgcc ctgcaaggct
    23521 gggaagttaa atccctgcgc gcaggaaaag ccaatatcag cgacagctac gtccttctgc
    23581 gtgacggaga ggcatttctg tttggcgcta acatcacgcc aatggccgtg gcctccacgc
    23641 atgtggtgtg cgaccctacc cgtacccgca agttacttct caaccagcgc gaactggact
    23701 cattgtacgg tcgcgtcaat cgagaaggct ataccgtagt ggcgctctcc ctgtactgga
    23761 aaaatgcctg gtgcaaagtg aaaatcggcg tcgccaaagg taagaaacag cacgataaac
    23821 gttcagatat caaagaacgc gaatggcagg tggataaagc acgtatcatg aaaaacgccc
    23881 accgttaaac ctgcactcca attattgacc agttcctcac cgcgcctccc tctccggcgg
    23941 cgcgaatgaa catcttattg gctattacat ccgacacaaa tgttgccatc ccattgctta
    24001 atcgaataaa aatcaggcta catgggtgct aaatctttaa cgataacgcc attgaggctg
    24061 gtcatggcgc tcataaatct ggtatactta cctttacaca ttggggctga ttctggattc
    24121 gacgggattt gcgaaaccca aggtgcatgc cgaggggcgg ttggcctcgt aaaaagccgc
    24181 aaaaaatagt cgcaaacgac gaaaactacg ctttagcagc ttaataacct gcttagagcc
    24241 ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc caaaagagat
    24301 cgcgtggaag ccctgcctgg ggttgaagcg ttaaaactta atcaggctag tttgttagtg
    24361 gcgtgtccgt ccgcagctgg caagcgaatg taaagactga ctaagcatgt agtaccgagg
    24421 atgtaggaat ttcggacgcg ggttcaactc ccgccagctc caccaaaatt ctccatcggt
    24481 gattaccaga gtcatccgat gaagtcctaa gagcccgcac ggcgcaagcc ctgcgggctt
    24541 ttttgtatct gcaatttgtc ccgcgaagtc cgaagagaac taattaaatc cgaacctttt
    24601 aggcccattg ataggcccaa cgaaaagctc tattgttttc gttgggccta aacgcatgga
    24661 gactccccat ggcaagaaaa accaagccgt taaccgatac ggaaatcaaa gccgccaaac
    24721 ctaaagatgc cgattaccag ctgtatgatg gtgacgggct tactctgtta atcaagtcca
    24781 gtggtagtaa gctctggcag ttccgttact atcgacctct gacaaagcag cgaaccaagc
    24841 agagcttcgg tgcctaccct gccgtctcac tttctgatgc gcgtaaactc agagccgaat
    24901 ctcgagtttt attggcaaaa gatattgatc ctcaggaaca tcagaaagaa caggtgagga
    24961 attctcaaga ggccaaaacc aacaccttct tgttagttgc cgagcgttgg tggaatgtga
    25021 agaaagccag cgtgacagag gactatgccg acgatatctg gcgctcgctt gagagagatg
    25081 tttttccagc aatcggtgat atcagtgtca ctgagattaa ggctcatact ctggttaaag
    25141 cagttcagcc ggttcaggct agaggtgcat tagaaactgt tcgccgtctt tgtcagcgta
    25201 ttaacgaagt catgatttat gcgcagaaca cgggcctgat tgatgcggtt cccagcgtaa
    25261 atatcggaaa agcgttcgag aagcctcaga aaaagaacat gccgagcatc cggcctgatc
    25321 aactgcctca actgatgcag acgatgcgaa cagcaaacat cagccaatca acacgatgcc
    25381 tgttcatgtg gcagcttctt actattaccc gtcctgccga agccgctgaa gctcgatggg
    25441 atgagatcga ttttgaagct aaagaatgga aaattcctgc agctcgaatg aagatgaacc
    25501 gggaccatac ggttccattg tctgatggag ctctcgctat tctggcaatg atgaagtcac
    25561 tcagtggtgg ccgagaattt atctttccta gtcgaatcaa accaacccag ccgatgaata
    25621 gccaaacagt gaatgcagca ctcaaacgtg ctggattggg aggcgttctc gtttctcatg
    25681 gcttgcgttc tatcgccagt accgccctta atgaacaagg cttcccacct gatgttattg
    25741 aagcggcgct ggctcatgtg gacaaaaatg aagtacgtcg cgcttacaat cgcagcgatt
    25801 atcttgagca gagacgcccg atgatgcagt ggtgggcaaa ccttataaaa gcggcagata
    25861 gtattcctac cgtttgtaac gaagtagcag gtcttagatt ggttgggtag tggatttaaa
    25921 atctcaagtg attatcctta gaaaagataa tcacttgaga taattgaaat ttttattgga
    25981 ttaaggcaaa gccatgctac attaattaag at
//
LOCUS       DAZUCE010000051        25532 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZUCE010000051 DAZUCE010000000
VERSION     DAZUCE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.051"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..537
                     /locus_tag="ACHY03_001786"
     CDS             <1..537
                     /locus_tag="ACHY03_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905025.1"
                     /translation="SFIIATIICITIKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHS
                     IWDYTFPEPGITVTVPSKQWVANIVKQGPTLVTRDAHAILAIVAFSQNELGVHSIEEL
                     TATQNGTAEMHVCDVQGFACAYQEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPD
                     YLDEYQEEVMKMMLSARQ"
     gene            660..1286
                     /locus_tag="ACHY03_001787"
     CDS             660..1286
                     /locus_tag="ACHY03_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905026.1"
                     /translation="MVGEYIGSVLLGPLLLPVLMSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(1325..1570)
                     /gene="zapB"
                     /locus_tag="ACHY03_001788"
     CDS             complement(1325..1570)
                     /gene="zapB"
                     /locus_tag="ACHY03_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFS3905027.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            1996..2841
                     /gene="glpF"
                     /locus_tag="ACHY03_001789"
     CDS             1996..2841
                     /gene="glpF"
                     /locus_tag="ACHY03_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFS3905028.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            2864..4372
                     /gene="glpK"
                     /locus_tag="ACHY03_001790"
     CDS             2864..4372
                     /gene="glpK"
                     /locus_tag="ACHY03_001790"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFS3905029.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            4602..5612
                     /gene="glpX"
                     /locus_tag="ACHY03_001791"
     CDS             4602..5612
                     /gene="glpX"
                     /locus_tag="ACHY03_001791"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFS3905030.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            5709..6455
                     /gene="fpr"
                     /locus_tag="ACHY03_001792"
     CDS             5709..6455
                     /gene="fpr"
                     /locus_tag="ACHY03_001792"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFS3905031.1"
                     /translation="MADWVTGKVTKVQNWTEALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPINKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(6460..6888)
                     /gene="uspD"
                     /locus_tag="ACHY03_001793"
     CDS             complement(6460..6888)
                     /gene="uspD"
                     /locus_tag="ACHY03_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFS3905032.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(6915..7214)
                     /gene="yiiS"
                     /locus_tag="ACHY03_001794"
     CDS             complement(6915..7214)
                     /gene="yiiS"
                     /locus_tag="ACHY03_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFS3905033.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(7426..7866)
                     /gene="yiiR"
                     /locus_tag="ACHY03_001795"
     CDS             complement(7426..7866)
                     /gene="yiiR"
                     /locus_tag="ACHY03_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS3905034.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            7967..8566
                     /gene="yiiQ"
                     /locus_tag="ACHY03_001796"
     CDS             7967..8566
                     /gene="yiiQ"
                     /locus_tag="ACHY03_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFS3905035.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            8674..9441
                     /gene="tpiA"
                     /locus_tag="ACHY03_001797"
     CDS             8674..9441
                     /gene="tpiA"
                     /locus_tag="ACHY03_001797"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFS3905036.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(9496..10251)
                     /gene="cdh"
                     /locus_tag="ACHY03_001798"
     CDS             complement(9496..10251)
                     /gene="cdh"
                     /locus_tag="ACHY03_001798"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFS3905037.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(10358..11347)
                     /gene="sbp"
                     /locus_tag="ACHY03_001799"
     CDS             complement(10358..11347)
                     /gene="sbp"
                     /locus_tag="ACHY03_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFS3905038.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(11666..12628)
                     /gene="pfkA"
                     /locus_tag="ACHY03_001800"
     CDS             complement(11666..12628)
                     /gene="pfkA"
                     /locus_tag="ACHY03_001800"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFS3905039.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(12809..13711)
                     /gene="fieF"
                     /locus_tag="ACHY03_001801"
     CDS             complement(12809..13711)
                     /gene="fieF"
                     /locus_tag="ACHY03_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFS3905040.1"
                     /translation="MNQSYGRLVSRAAIAATVMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(13860..14360)
                     /gene="cpxP"
                     /locus_tag="ACHY03_001802"
     CDS             complement(13860..14360)
                     /gene="cpxP"
                     /locus_tag="ACHY03_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFS3905041.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            14510..15208
                     /gene="cpxR"
                     /locus_tag="ACHY03_001803"
     CDS             14510..15208
                     /gene="cpxR"
                     /locus_tag="ACHY03_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFS3905042.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            15205..16578
                     /gene="cpxA"
                     /locus_tag="ACHY03_001804"
     CDS             15205..16578
                     /gene="cpxA"
                     /locus_tag="ACHY03_001804"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFS3905043.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETLKANQLWGEVLDNAAFEAEQMGKSFSV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(16684..17358)
                     /gene="yiiM"
                     /locus_tag="ACHY03_001805"
     CDS             complement(16684..17358)
                     /gene="yiiM"
                     /locus_tag="ACHY03_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFS3905044.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDIRDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(17507..18490)
                     /gene="kdgT"
                     /locus_tag="ACHY03_001806"
     CDS             complement(17507..18490)
                     /gene="kdgT"
                     /locus_tag="ACHY03_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFS3905045.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(18750..19370)
                     /gene="sodA"
                     /locus_tag="ACHY03_001807"
     CDS             complement(18750..19370)
                     /gene="sodA"
                     /locus_tag="ACHY03_001807"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFS3905046.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            19655..20689
                     /gene="rhaT"
                     /locus_tag="ACHY03_001808"
     CDS             19655..20689
                     /gene="rhaT"
                     /locus_tag="ACHY03_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFS3905047.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLISTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKLLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(20686..21534)
                     /gene="rhaR"
                     /locus_tag="ACHY03_001809"
     CDS             complement(20686..21534)
                     /gene="rhaR"
                     /locus_tag="ACHY03_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFS3905048.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(21608..22444)
                     /gene="rhaS"
                     /locus_tag="ACHY03_001810"
     CDS             complement(21608..22444)
                     /gene="rhaS"
                     /locus_tag="ACHY03_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFS3905049.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            22732..24201
                     /gene="rhaB"
                     /locus_tag="ACHY03_001811"
     CDS             22732..24201
                     /gene="rhaB"
                     /locus_tag="ACHY03_001811"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFS3905050.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTTGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPETMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            24198..25457
                     /gene="rhaA"
                     /locus_tag="ACHY03_001812"
     CDS             24198..25457
                     /gene="rhaA"
                     /locus_tag="ACHY03_001812"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFS3905051.1"
                     /translation="MTTQLEQAWELAKQRFVAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEVAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSRRG"
BASE COUNT         6328 a         6520 c         6448 g         6236 t
ORIGIN      
        1 tcatttatta tcgccacaat catctgtata acgataaaaa aaagaagcag ccgcagcttt
       61 attcgcggca cttgttggtt aatgggatta tttcttatct atagcagcta tacggtgtgg
      121 gaacgacaca gcatatggga ttatactttc ccggagccag gtatcaccgt cacggttccg
      181 agcaaacagt gggttgccaa tatagtaaaa caaggaccaa ccctggtgac ccgggatgcc
      241 catgctattt tagctattgt tgccttctca caaaatgaac tcggtgtgca ttcaatcgag
      301 gaacttaccg cgactcaaaa cggaactgca gaaatgcatg tttgtgatgt tcagggattt
      361 gcctgcgctt atcaggaagg agtgcaaaca atgagtgatg gcaaagtaag acatgctatt
      421 ttcatgactc tactggataa cactaatttg atacaactgg tcgcagccat cgacccagat
      481 tatttggacg aatatcaaga agaagtaatg aaaatgatgc tttctgctcg tcaatgatct
      541 gatgtgcatg ttaataataa tggattgata tgagatacgt tatcttatta atactatgga
      601 taataactgt ttttcttagt catcttcagc acagtaactt gcttactgga gatcctggga
      661 tggttggcga gtatatcggt tcagtacttc ttgggccgtt attacttccc gtgcttatgt
      721 caggtattct gtgcacattc actaaaaaaa cgcgtaactt cgccagtttt actcgcgggt
      781 gctgttgggt attgggagtc ctgcttttat ccaacgtcgg caatacgttt cgtctgttca
      841 ccccgtggca ctatacgttt gagaaggcag ctatttccgt gactgtccca aaccgacact
      901 ggaatacggt ttctattagt actgataaaa ccattgatat aagaagtgaa gataatagcg
      961 tctttatttc tgctttccgc ctgcccgcag gacgtagcgc cgatgactcg ctggaagagt
     1021 taaagaagat gcagcgagac aatctcaagg atcaatacaa tgaagaaacc ttccagtttc
     1081 atgattgtaa tgccaaacat ttcacctgta agtatcagga tgtgttgata aattttgatg
     1141 gtcagcagaa aagaaccatc agtgtttatc ttgaagacac ccccagggct gtggggatca
     1201 ttgcattaat ggagccagat acggcagata aatatcgcca acaggcgatg gagattatgt
     1261 tgtctgctaa aaatacagtg aagtaaaaaa taaggcgcct tgcggcgcct ctgcgtggaa
     1321 gagattaaac ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct
     1381 gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt
     1441 gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca
     1501 gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctaatg
     1561 acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc tcgtcccgat
     1621 taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg tatgaagccg
     1681 cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt ttcatccttc
     1741 tcctggtgac ataatccaca ccaatcgaaa atgttaataa atttgttgcg cgaatgatct
     1801 aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc tcattttatg
     1861 acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac gataagttta
     1921 cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca aacattaact
     1981 cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat tgctgaattc
     2041 ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact aaaagtcgct
     2101 ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt ggcaatggcc
     2161 atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac cattgcattg
     2221 tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc acaagttgcc
     2281 ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt tttcgacttc
     2341 gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc tggcactttc
     2401 tctacttacc ctaatcctca tatcaatttt gtgcaggcct tcgcagtgga aatggtgatt
     2461 accgctatcc tgatgggact gatcctggcg ttaacggacg atggcaacgg tgtaccacgc
     2521 ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc atctatgggc
     2581 ccattgacgg gttttgccat gaacccagcg cgtgacttcg gtccgaaagt ctttgcctgg
     2641 ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta cttcctggtg
     2701 ccgctttttg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt
     2761 ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggagaccac aactccttca
     2821 gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg aaaaaaaata
     2881 tatcgttgcg ctcgaccagg gcaccaccag ctcccgcgcg gtcgtaatgg atcacgatgc
     2941 caatatcatt agcgtgtcgc agcgcgaatt tgagcaaatc tacccaaaac caggctgggt
     3001 agaacacgac ccaatggaaa tctgggccac ccaaagctcc acgctggtag aagtgctggc
     3061 gaaagccgat atcagttccg atcaaattgc agctatcggt attacgaacc agcgtgaaac
     3121 cactattgtc tgggaaaaag aaaccggcaa gcctatctat aacgccattg tctggcagtg
     3181 ccgtcgtacc gcagaaatct gcgagcattt aaaacgtgac ggtttagaag attatatccg
     3241 cagcaatacc ggtctggtga ttgacccgta cttttctggc accaaagtga agtggatcct
     3301 cgaccatgtg gaaggctctc gcgagcgtgc gcgtcgtggt gaattgctgt ttggtacggt
     3361 tgatacgtgg cttatctgga aaatgactca gggccgtgtc catgtgaccg attacaccaa
     3421 cgcctctcgt accatgttgt tcaacatcca taccctggac tgggacgaca aaatgctgga
     3481 agtgctggat attccgcgcg agatgctgcc agaagtgcgt cgttcttccg aagtgtacgg
     3541 tcagactaac attggcggca aaggcggcac gcgtattcca atctccggga tcgccggtga
     3601 ccagcaggcc gcgctgtttg gtcagttgtg cgtgaaagaa gggatggcga agaacaccta
     3661 tggcactggc tgctttatgc tgatgaacac tggcgagaaa gcggtgaaat cagaaaacgg
     3721 cctgctgacc accatcgcct gcggcccgac tggcgaagtg aactatgcgt tggaaggtgc
     3781 ggtgtttatg gcaggcgcat ccattcagtg gctgcgcgat gagatgaagt tgattaacga
     3841 cgcctacgat tccgaatatt tcgccaccaa agtacaaaac accaatggtg tgtatgtggt
     3901 tccggcattt accgggctgg gtgcgccgta ctgggacccg tatgcgcgcg gggcgatttt
     3961 cggtctgact cgtggggtga acgctaacca cattatacgc gcgacgctgg agtctattgc
     4021 ttatcagacg cgtgacgtgc tggaagcgat gcaggccgac tctggtatcc gtctgcacgc
     4081 cctgcgcgtg gatggtggcg cagtagcaaa caatttcctg atgcagttcc agtccgatat
     4141 tctcggcacc cgcgttgagc gcccggaagt tcgcgaagtg actgcattgg gtgcggccta
     4201 tcttgctggt ctggcggtcg gtttctggca gaacctcgac gagctgcaag agaaagcggt
     4261 gattgagcgc gagttccgtc caggcatcga aaccactgag cgtaattacc gttacgcagg
     4321 ctggaaaaaa gcggtcaaac gcgcgatggc gtgggaagaa cacgacgagt aatgtcaggc
     4381 acctgaatcg attgcctgat gccgatcgcc tgatgcgacg cttacgcgtc ttatcaggcc
     4441 tacgagccaa tgccattttg tgtaggcggg ataaggcgtt cacgccgcat ccggcatcac
     4501 gcagactctg aaaccgtgcg ggggcaaccc cgcacacatc aataatccca cccttcccct
     4561 gtgctacact tcgcgccatt ccttactgct tagagtttgc tatgagacga gaacttgcca
     4621 tcgaattttc ccgcgtcacc gaatctgcgg cgctggctgg ctacaaatgg ttaggacgcg
     4681 gcgataaaaa caccgcggac ggcgcggcgg taaacgccat gcgtattatg ctcaaccagg
     4741 tcaacattga cggcaccatc gtcattggtg aaggtgaaat cgacgaagca ccgatgctct
     4801 acattggtga aaaagtcggt actggtcgcg gcgacgcggt agatattgct gttgatccga
     4861 ttgaaggcac gcgcatgacg gcgatgggtc aggctaacgc gctggcggtg ctggcagtag
     4921 gcgataaagg ctgtttcctc aacgcgccag atatgtatat ggagaagctg atcgtcggac
     4981 cgggagccaa aggcaccatt gatctgaacc tgccgctggc ggataacctg cgcaatgtag
     5041 cggcggcgct cggcaaaccg ttgagcgaac tgacggtaac gattctggct aaaccacgcc
     5101 acgatgccgt tatcgctgaa atgcagcaac tcggcgtacg cgtatttgct attccggatg
     5161 gcgacgttgc ggcctcaatt ctcacctgta tgccagacag cgaagttgac gtgctgtacg
     5221 gtattggtgg cgcgccggaa ggcgtggttt ctgcggcggt gatccgcgca ttagatggcg
     5281 acatgaacgg tcgtctgctg gcgcgtcatg acgtcaaagg cgacaacgaa gagaatcgtc
     5341 gcattggcga gcaggagctg gcacgctgca aagcgatggg catcgaagcc ggtaaagtat
     5401 tgcgtctggg cgatatggcg cgcagcgata acgtcatctt ctctgcaacc ggtattacca
     5461 aaggcgatct gctggaaggc attagccgca aaggcaatat cgcgactacc gaaacgctgc
     5521 tgatccgcgg caagtcacgc accattcgcc gcattcagtc catccactat ctggatcgca
     5581 aagacccgga aatgcaggtg cacattctct gagtgatttg atcgattgag ccttccagtc
     5641 cttcgggact ggaatttttt tgttcggaga acgaagataa ggcaagtcaa tcaaaacagg
     5701 agaaaaacat ggctgattgg gtaacaggca aagtcactaa agtgcagaac tggaccgaag
     5761 ccctgtttag tctcaccgtt cacgctcccg tgcttccgtt taccgccggg caattcacta
     5821 aacttggcct tgaaattgac ggcgaacgcg tccagcgcgc ctactcctat gttaactcgc
     5881 ccgataatcc cgatctggag ttttacctgg tcaccgtccc cgatggcaaa ttaagcccac
     5941 gattggcggc actgaaaccg ggcgatgaag tgcaggtggt tagcgaagca gctggcttct
     6001 ttgttctgga tgaagtacca gattgcgaaa cgctatggat gctggcaacc ggtacagcga
     6061 ttggccctta tttatcgatt ctgcaactag gcaaagattt agatcgcttc aaaaatctgg
     6121 tcctggttca cgccgcacgt tatgccgccg acttaagcta tttgccactg atgcaagaac
     6181 tggaaaaacg ctacgaaggg aaactgcgca ttcagacggt ggtcagtcgg gaaacggcgg
     6241 cggggtcact caccgggcgg ataccggcgt taattgaaag tggtgaactg gaaagcgcga
     6301 ttggcctgcc gataaataaa gaaaccagcc atgtgatgct gtgcggcaat ccacagatgg
     6361 tgcgcgatac ccaacagttg ctgaaagaga cccggcagat gacgaaacat ttacgtcgcc
     6421 gaccgggcca tatgacagcg gagcattact ggtaagcggt tacttatcga taaacggcac
     6481 gatgagcaaa tccgcactca acttattgat catcccgcga tatgccggca tcaaacggtt
     6541 gataaatgag tggtgatgac cacagacaag gaggtcgcac tgctcttttt gcataatttc
     6601 cagcagtgtt tccggcattt ctccgcgttc aatacgcagt tttgtcttcg gccattgaat
     6661 atttttcgtc agtttataca gcttgttatc cgacttattc ttcaacaatt gaagaatatc
     6721 ctctgttgca gggaagtaga tacccggata caactcgctt aagccatcat caatatgaat
     6781 taacgtcaga tgagcgtcat tatgtctggc gagctccagg gctttattca ccagtaaggc
     6841 atcttcttca ttcccggaaa ttgccacgcc aatgtgttta taagccatgt ttaactcctt
     6901 ctaaagcaac tccatcaagc taacgaacgc agtgataact caaaaataat catctcggcc
     6961 tggcatgaga aggtgaaggc cgcatcaagc tcaacttgcc cctccagatc cttaatctta
     7021 taggccacgc tggccccttc atctgcagag gatgcagcgg cggcaagctc tttcagtttg
     7081 gtcataaagg actctgcttc ttcgcgggtg gcaaaaaagc gcgaaaactt actggtacag
     7141 ttgtcgttat caatcaccgt accgatatca atcgcacatc ctttagtact gcatttatct
     7201 acgacatctt tcatagggca cctctgtata ttcgcctctc tgtcgattac gacttaagaa
     7261 taatgcgccg ttaagggaag aacaaacaag taatgatctt atggattacc attttttttg
     7321 atattgctta aggtgaagag ttcagaagga ttttccgggc attgtcattt gcatcgtgga
     7381 aataacaatc ccggaaaaaa atagcagtaa gagatatatt aaaggttaat tacttgattt
     7441 attgtcggct ttatacttca catcctgggt atctttacca tatttatttt cgccttgtgt
     7501 gccaacaaac gctccaagat cgataagcat catcaccaga atcaacgtcg ggacaaaacg
     7561 ccccaccgcc cattgccaga cacccggtaa aatcgcccag ttacccgcca gtagcatcca
     7621 cgccacaatc atcagaaatg cccatgcgcc ggaacgcccg cgatcatgca agcgcttaac
     7681 ggttactgcc gctgttggcc agagcaagca cacaaggcaa aacgccgcgg tctgaatatc
     7741 gagtaaattt ttacccgcca gtgagaaaag caccagcatg cctgcgaacc acaggcctat
     7801 ccaaatccag aaatcacggc gtccaatacg ccctttaaat gagaataacc attgctgtat
     7861 ggtcatgtaa gttccttgat ggttgtcttt tccaggattc tacccttttg acaagggcga
     7921 cagatatcgt tttaatcgga gccagtcata acaaaaggta ctgtcaatga agccagggtg
     7981 tacgctgttt tttctcttat gttctgcatt aaccgttaca acaacggcgc atgcacaaac
     8041 accagatacg gcaacgaccg cgccttatct gctggctgga gcccctactt tcgatctctc
     8101 catcagccag tttcgagaag actttaacag ccagaatccc agcctgccac tgaacgaatt
     8161 tcgtgccatc gacagcagtc ccgacaaagc caatctcact cgtgctgcaa gtaaaattaa
     8221 cgagaacttg tatgcttcta cagcgctgga gcgcggtacc ttaaaaatca aaagcattca
     8281 aatgacctgg ctacccatcc aggggccaga gcaaaaagcc gcgaaagcga aagctcagga
     8341 atatatggca gcggtgatcc gcacactcac cccattaatg accaaaacac aaagccagaa
     8401 aaaactgcag tcgctactaa cggcggggaa aaacaaacgt tattacaccg agacagaagg
     8461 tgcactgcgt tatgttgtcg cggacaacgg cgaaaagggg ctgaccttcg ctgttgaacc
     8521 gattaagctg gcgctatctg aatcgcttga aggtttgaat aaatgacaaa aagcaaagcc
     8581 tttgtgccga tgaatctcta tactgtttca cagacctgct gccctgcggg gcggccatct
     8641 tcctttattc gcttataagc gtggagaatt aaaatgcgac atcctttagt gatgggtaac
     8701 tggaaactga acggcagccg ccacatggtt cacgagctgg tttctaacct gcgtaaagag
     8761 ctggcaggtg ttgctggctg tgcggttgca atcgcaccac cggaaatgta catcgatatg
     8821 gcgaagcgtg aagctgaagg cagccacatc atgctgggtg cgcaaaacgt ggacctgaac
     8881 ctgtccggcg cattcaccgg tgaaacctct gctgctatgc tgaaagacat cggcgcacag
     8941 tacatcatca tcggtcactc tgaacgtcgt acttaccaca aagaatctga cgaactgatc
     9001 gcgaaaaaat tcgcggtgct gaaagagcag ggcctgactc cggttctgtg catcggtgaa
     9061 accgaagctg aaaacgaagc gggcaaaact gaagaagttt gcgcacgtca gatcgacgcg
     9121 gtactgaaaa ctcagggtgc tgcggcattc gaaggtgcgg ttatcgctta cgaacctgta
     9181 tgggcaatcg gtactggcaa atctgcaact ccggctcagg cacaggctgt tcacaaattc
     9241 atccgtgacc acatcgctaa agttgacgct aacatcgctg aacaagtgat cattcagtac
     9301 ggcggctctg taaacgcgtc taacgctgca gaactgtttg ctcagccgga tatcgacggc
     9361 gcgctggttg gtggtgcttc tctgaaagct gacgctttcg cagtaatcgt taaagctgca
     9421 gaagcggcta aacaggctta agtctgacag gtgccggatt tcatatccgg cacttacttt
     9481 ccttaactct tcgccttaac gcaaaatctc acactgatga tcctgaattt cctcggctga
     9541 agcacggttc agcgtcagta gatttcgttg tgtcgccagc aatacaaatg agttatcact
     9601 ctgccgcacc atcgccagcc cgtagcttcc catatgttcc cgcgcctcag gcacttcttc
     9661 cgccagcatc ataaatgggc tgcgttgtac cagttcgctt tccgttaccc gacgcgccag
     9721 gtattcatgc ccgcgcaaac cacctggcag tggcaaccag cggctgctga tgttcgccag
     9781 attgttatcc agctgttcgc gcacatcagg acgaatacaa gagatatgaa tatgaaaatg
     9841 gttttgcgta cgcccggtgc gggagttgat cgccaaagaa accgcgcgat cgggaaccgg
     9901 ctggccgtat tttttgctca taaaatcacg cgcctgccag gccaaccaaa agaagttcgg
     9961 cgttgaagga tcggtcaaca aaggactttc agtaccgtta atacgatacg ttggcatcaa
    10021 cagatattgc agtgggccat taagatcttt taaaaccacg tatccggcat tgggtttgac
    10081 ttccgcacat ggcgaaggat tttgattttg ctgctgattg ggcaaacatt cctcaaggac
    10141 aatcttacgt aatgtatccg actcttcacc ggttaatttc cagtaaccaa taccggcagc
    10201 cacaacggcg ataactatca tcaccaaaaa aagaagaccc gcttttttca tctttttttc
    10261 cctgtacctc aaagagatgc aagggtaacg caaaatcgtg acaaataaaa aacccggtcg
    10321 cgagaaacca ccgggtttga taattatcct ggagaaatca gcgtttgctg atctgatcga
    10381 acgtaccgcc gttagcaaaa tgctcttttt gcgctttcgt ccagccgccg aactcttcat
    10441 caatggtgaa taacttcagc tttggaaacg cattttcgta ctttttcgcc acctcagcgt
    10501 cgcgcggacg gtagtagttt ttcgcggcaa tttcctgacc ttctggcgag tagagatatt
    10561 tcaggtaggc ttccgccacc tctttagtac cttttttctc gaccacttta tcgaccaccg
    10621 acacggttgg ctctgcgagg atagactcac tcggcgtgac aatttcgaat ttatctttcc
    10681 ccagttcatt cgctgccagt agagcttcgt tttcccaggc gatcagtaca tcgccaattc
    10741 cgcgctcgac aaaagtgtta gtggagccgc gcgcgccaga atccagaact tcgacgtttt
    10801 tatacagtgc ccgaacaaaa tcctgtgctt ttgcctgatc gttgttgttg tgatgcagcg
    10861 cgtagcccca ggcagccagg taattccagc gcgcgccacc agagcttttc ggattaggcg
    10921 tgatcaccga aacacccggt ttaatcagat cgttccagtc atggatctgc ttcggatttc
    10981 ccttacgtac caggaaaaca atggtggaag tgtacggtgc ggagttatcc ggcagacgtt
    11041 tgatccactc tttatcaatc cgcccgcgtt ccgcaatcgc gtccacgtca taggccagag
    11101 ccagcgtgac aacatcagct tcaataccgt tgattaccga cgttgcttgt ttgcctgaac
    11161 caccgtgtga ctgacgaatc accacgttat cgccagtttg ctgtttccag tgggcgctga
    11221 atgccttgtt gtactgttcg tacaattcgc gcgttggatc atatgaaacg ttaagaagct
    11281 gaatatcctt tgccataacg ctggttgccg ccagcaaaaa tgttaaccct acgccccact
    11341 tgttcatcgc ccgactctct tatgttgtgt tgtgatgagc aaagcgtgcc agaaggttaa
    11401 ccaaacatta aagaataaaa aaagattggc tataacttgc gggtatatgt tgggggttaa
    11461 aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc gctttatcga tgccggatgc
    11521 ggcgcgaacg ccttatccgg cctacaaaag tttgtaaatt caataaattg tagaattcac
    11581 gtaggcctga taagcgaagc gcatcaggca tttttgcttc tgtcatcggt tccaggctaa
    11641 aggaatctgc ctttttccga aatcattaat acagtttttt cgcgcagtcc agccagtcgc
    11701 ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga accagctgtt
    11761 cgttctggat acctacgcaa cgaccgccgt aacctgccag cagcagatcg atagcgtaag
    11821 cgcccatacg ggaagccaga atacggtcgt aaggcaccgg agaaccaccg cgctggatgt
    11881 ggcccagcac agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa tgcgccagtt
    11941 cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc gtgtttttta cctttcgcga
    12001 tacccgcttt gatttcgttt accaggtctt cacggctgaa ttcaacttcc ggaaccacaa
    12061 cgaattcaca gccaccggca atggccgcag ccagcgtcag atcgccacaa taacggccca
    12121 tcacttccac cacggaaata cgctggtgag aagaagaggt gtcacgcaga cggtcgatcg
    12181 cttctacaac ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg cctttgatgt
    12241 cgttgtcaat agtgcccggc aggccgatgc acgggaagcc catttcggtc agacgcattg
    12301 cacccatgta ggaaccgtca ccgccgataa ccaccagcgc atcgatccca cgttttttca
    12361 ggttttcgat agccacggcg cggatgttct cgtcgcggaa ttccgggaaa cgcgcagaac
    12421 cgaggaacgt accgccacgg ttgatcatgt cagaaacgct gtaacggtct agctgtacca
    12481 tacggtcttc atacagaccc agatagccgt cataaatgcc cattacttcc agaccttctg
    12541 tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat gcctggcgca tcaccgccgc
    12601 ttgtcaacac accgattttc ttaatcatga ctacctctga actttggaat gcaaaatgaa
    12661 atctgttgcc ggaagtcttc ttgcacatcg aagtgatcca acgaatgtgc aaatagtata
    12721 acaatcactt cctgctgaat tgattcaggt caggccaaat ggcggtattt tatacacaaa
    12781 atgcgggtct ggctctcttt tatactgatt atgaaagcat agaccgttta ccctccctgg
    12841 gtacgacgga acaggggtcc tgatggataa tcacatccga tcccggaaaa cgccgtaaaa
    12901 tagcctgctc tacctgatcc gccaccatat gtgcctgaac caaaggcaga gagtcttcca
    12961 tttccaaatg aatctgaata aagcgggtcg gccctgactg ccgcgtgcga agatcgtgag
    13021 cgccgctaac acccggccag gaagtcacga tatcaataat ttcttgccgt tcctcatcag
    13081 gtaatgcgcg atccagtaat gactgtaccg cctcatatcc catgcgtaac gcgctatata
    13141 aaatatagat gccgattccc aatgcaaaca gagcatcggc gcgatgccag ccgtaccagg
    13201 acaaccccag cgccagcaga attgcgccgt tcatcataac atcagactgg taatgtagca
    13261 tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac ccaacgctga aacgagacaa
    13321 ggataatcgt acaaattagc gccacaattg tcacgataac cccgacgcct ggatctgtca
    13381 tcggtgttgg agatatcaga tgttgaatgc ctgtcaaaaa caggaatagt gccgaaccgg
    13441 agataaacat actttgcgcc agcgccgcga gggactcagc tttaccgtga ccaaacgagt
    13501 gattatcgtc ggcaggttgc agggaatatc gcaccaccaa taaattcgtc aacgacgcgc
    13561 cgatatccac cagcgaatcc accagcgcgg cgagaatact caccgacccg gtataccacc
    13621 atgcaaaaat tttaatcagc aatagcagcg aagccatcac cgtcgcagca atcgccgccc
    13681 gactgaccag ccgtccataa gattgattca taaatactcc cgctatcaac tgacgctagt
    13741 ataacggaag caaatcatct gcaatgcatt aagcagcagg caaattgagg ataaaaaaaa
    13801 cccccacatc atgtggggga agacagggat ggtgtctatg gcaaggaaaa cagggtttac
    13861 tactgggaac gtgagttgct actactcaat agcttcaacg atgaactttt ttgccattgc
    13921 gtcacgtcac gcaactgctc cattcgttgt tgatgtttct cgtttaaaac cgcttgctgc
    13981 tccggcgtta acaggcgata catttggttg cggacttttg ccatctcaac ctgacgagca
    14041 atttgctcat tcgccatttt ttctgcctgt gcgcgcacag cgttttcatc aaaattttct
    14101 gcggtgacaa ggcgatgcat tgtctccagt tcgctaacat taacaggagg ctgttcgtgc
    14161 cgggcctgtt gcataagatc tcgcatctgc tgacgctgat gttcggttaa acttatgccg
    14221 tcgaacatat ggctctgcgt actgcgctgc gtaagttctt cacccggatg ccagttatcg
    14281 cctgaaccga cttcagcagc gtggcttaat gaactgactg ccagcgttga ggccatgacg
    14341 gcagcggtaa ctatgcgcat catttgctcc caaaatcttt ctgtcgcgat tcaacgatag
    14401 agagtttacg attcaggctg caaacatgcg tcagggggtg taaaacaacg taaagtcatg
    14461 gattagcgac gtctgatgac gtaatttctg cctcggaggt atttaaacaa tgaataaaat
    14521 cctgttagtt gatgatgacc gagagctgac ttccctatta aaggagctgc tcgagatgga
    14581 aggcttcaac gtgattgttg cccacgatgg ggaacaggcg cttgatcttc tggacgacag
    14641 cattgattta cttttgcttg acgtaatgat gccgaagaaa aatggtatcg acacattaaa
    14701 agcacttcgc cagacacacc agacgcctgt cattatgttg acggcgcgcg gcagtgaact
    14761 tgatcgcgtt ctcggccttg agctgggcgc agatgactat ctcccgaaac cgtttaatga
    14821 tcgtgagctg gtggcacgta ttcgcgcgat cctgcgccgt tcgcactgga gcgagcaaca
    14881 gcaaaacaac gacaacggtt caccgacact ggaagttgat gccttagtgc tgaatccagg
    14941 ccgtcaggaa gccagcttcg acgggcaaac gctggagtta accggtactg agtttaccct
    15001 gctctatttg ctggcacagc atctgggtca ggtggtttcc cgtgaacatt taagccagga
    15061 agtgctgggc aaacgcctga cgcctttcga ccgcgctatt gatatgcaca tttccaacct
    15121 gcgtcgtaaa ctgccggatc gtaaagatgg tcacccgtgg tttaaaacct tgcgtggtcg
    15181 cggctatctg atggtttctg cttcatgata ggcagcttaa ccgcgcgcat cttcgccatc
    15241 ttctggctga cgctggcgct ggtgttgatg ttggttttga tgttacccaa gctcgattca
    15301 cgccagatga ccgagcttct ggatagcgaa cagcgtcagg gtctgatgat tgagcagcat
    15361 gttgaagcgg agctggcgaa cgatccgccc aacgatttaa tgtggtggcg gcgtctgttc
    15421 cgggcgattg ataagtgggc accgccagga cagcgtttgt tattagtgac caccgaaggc
    15481 cgcgtgatcg gcgctgaacg cagcgaaatg cagatcattc gtaactttat tggtcaggcc
    15541 gataacgccg atcatccgca gaagaaaaag tatggccgcg tggaactggt cggtccgttc
    15601 tccgtgcgtg atggcgaaga taattaccaa ctttatctga ttcgtccggc cagcagttct
    15661 caatccgatt tcattaactt actgtttgac cgcccgctat tactgctgat tgtcaccatg
    15721 ctggtcagta cgccgctgct gttgtggttg gcctggagtc tggcaaaacc ggcgcgtaag
    15781 ctgaaaaatg ctgccgatga agttgcccag ggaaacttac gccagcaccc ggaactggaa
    15841 gcggggccac aggaattcct tgccgcaggt gccagtttta accagatggt caccgcgctg
    15901 gagcgcatga tgacttctca gcagcgtctg ctttctgata tctctcacga gctgcgcacc
    15961 ccgctgacgc gtctgcaact gggtacggcg ttactgcgcc gtcgtagcgg tgaaagtaag
    16021 gaactggagc gtattgaaac cgaagcgcaa cgtctggaca gcatgatcaa cgatctgttg
    16081 gtgatgtcac gtaatcagca gaaaaacgcg ctggtaagcg agacattgaa agctaaccag
    16141 ttgtggggcg aagtgctgga taacgcggcg ttcgaagcag aacagatggg caagtcgttt
    16201 tctgttaact tcccgcctgg gccgtggccg ctgtacggca acccgaacgc cctggaaagt
    16261 gcgctggaaa acattgttcg taatgctctg cgttattccc atacgaagat tgaagtgggc
    16321 tttgcggtag ataaagacgg tatcaccatt acggtggacg acgatggtcc tggcgttagc
    16381 ccggaagatc gcgaacagat tttccgtccg ttctatcgga ccgatgaagc acgcgatcgt
    16441 gaatctggcg gtacgggctt agggctggca attgttgaaa ccgccattca gcagcatcgt
    16501 ggctgggtga aagcagaaga cagcccgctg ggcggtttac ggctggtgat ttggttgccg
    16561 ctgtataagc ggagttaaac tccgcatttg taggcaggat aaggcgttta cgccgcatac
    16621 ggcatttgag caggatgcct gatgcgacgc tgatagcgtc ttatcaggcc tacactccgg
    16681 gggttatttt ccccacaacc ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt
    16741 ttgcatcgtt cgcgtccagc ttttcgataa cccggcagcg gagagtaaac ggtgatactg
    16801 atcgtcatca aacggcatat gccatgcgat ggcggcagcc tcctgcacgg tcacatcgct
    16861 gacacgggaa accaactcca gcggcgcgtc cgcagacact ttccctggcg ctatcacgct
    16921 atacaaccat cccactttac ccgtgttttg catcaactgc gctatatctc tgatatcaaa
    16981 atggtaattg agtttatagc aaggcgagcg cggctggctg acctgaatta atgcctctcc
    17041 ccagcggaaa atatcgccca tataaacatt actttccgtc agaccgtcgg ttgagagatt
    17101 ttcaccaaac gcaggcgcaa caaacaactc cgcctgttcc ggaaattccc gcgcccagta
    17161 gagataatgc tcacgaggat aatgacacag cgctctgtct ggcccgccgt gaaccttttt
    17221 ctccgcctgc tcgtcacctt ccagccccag ctccgtcagc atcaactcac catcaacctg
    17281 gattttggca attgcgctgg gtttgccttc gggataagcc tgaatcttgc ctgtgtatac
    17341 atcaaccgga tatcgcatct gttccctctc cacgagaaat ttccccatta gaccatattt
    17401 attttatgtc aggcgtgctg ttttcgcggt aagtatccag gcaggaactc aatagcaaac
    17461 cggacgaatg ttagttcatc cggctgtggg gattaaggaa gcaggtttat ttcaccgtac
    17521 ctaaaatttc gattttcgct gctctggctt tgactttacg tgaccagata gaggtaagaa
    17581 ttggcaccag aatcgaagtc acaatgaccg ccgtcgctac cagcgaagtt gctgccggag
    17641 ccatcggttt aaacgcaggc accatttctg caatcagcac aggtgtcgct accgcggccc
    17701 ctgcggaact ggaagcggca attccggcag tgccatcgcc accgccaatc aatttatcgg
    17761 cgataatcaa cggaataccg gtcacgataa ttactgccac acctaacagg atccccagca
    17821 aacctgtctg ggcaattaca gtcaaatcaa tggtattgcc tagtgcgaag gcaaagaatg
    17881 gaatcagcgt ctgcaccgct ttgctgaaaa attctcgtaa ttcagggtca aggttcccaa
    17941 gggcaaagcc caccaggaac ggtaatactg cgccgacgaa aacatgcggt tcaaacgagg
    18001 caatcccggc agtgcccaga ataatcatcg tcatgagcgg cccggactcc aacgacatca
    18061 acacaaatgc cccagcttct tcttttgtgc cgtactgctg catgatggaa gcgtaaagtc
    18121 cgccattggt catatccatc gccgccacca gcgccagcgt tgaaagtccg gcaaagaatc
    18181 caacttcaac accatgttcc ggaataatac gcgaggcaat tgccgcaacc acccacgcga
    18241 cggcaatttt agttaccacc agcgtaccgg atttacgcag taccgttccc gtcgcgctta
    18301 attttattga cgcccccatg caaaaaaacc acaccgccag aatgggcacc gtaccggtaa
    18361 tcatcccgtt ggtgaatgat ccaaaatatt tccccgcccc cggcgagaag gtgtggcaca
    18421 gtgcgccaag gaatagcggg acgagcatca tcccccccgg gattttctca atcgagcgtt
    18481 ttatctgcat ttccatcacc tgtcggcata tttttaaggg ctgatgcgta attcctcaat
    18541 ggagcatcag tgttgatgga aacgaagata gacattcatt taatcaataa aagtgatcaa
    18601 aaccacatca attaaaacgc tgttttattt ttatcggatc attgttttta ttttgaaatt
    18661 gatcacaaaa aaaccaccgc cattggcgat ggttcatttt ttaagctgat atgcggcgta
    18721 aacgcctcat tgcagcagac ggcaaatgat tattttttcg ccgcgaaacg tgccgctgct
    18781 tcgtcccagt tcaccacgtt ccagaactct ttgatgtagt ccggacggcg gttctggaat
    18841 ttcaggtagt aagcatgttc ccacacatcc aggcccagaa tcgggaagcc ggaagcgcca
    18901 gaaatagctt cacccatcag cggggagtcc tggttagcag tagaaaccac cgccagttta
    18961 tcgcctttca gcaccagcca tgcccagccg gaaccaaagc gggaagctgc cgctttttca
    19021 aattctgctt tgaagttatc tacggaaccg aaatcacgtt cgatagccgc tttcaggtca
    19081 ccctgcaggg tggtgccttt tttcagacct ttccagaaca ggctgtggtt agcgtgaccg
    19141 ccagcgttgt tgcgcagtac ggttttcttg tctgctggca gctggtccag tttggtgatc
    19201 aactcttcaa ccggcaggtt ggcaaattct ggcaggcttt ccaacgccgc gttggcgttg
    19261 ttgacgtagg tctgatggtg tttggtgtgg tggatttcca tggtctgctt atcgaagtgc
    19321 ggttccaggg catcgtaagc atacggcagg gatggcaggg tatagctcat attcatctcc
    19381 agtattgtcg ggcggccgat tgttaatgcc gcgtaagcag ttggttcatt atagttaatt
    19441 aaatgatatt gaaaatgatt atcaatgccg tacttttcgt aagggtatgg ttttgcagga
    19501 aaatgcccga gatgtgaagc aaatcaccca cttaatgccg tgattgccag taaatcgaca
    19561 acggcggcaa caggcgaaag gttaatcgac agcacgattt ttacactcat ctcgtcggag
    19621 atgtgacgcg acgaaaaatg atgaggataa gaagatgagt aacgcgatta cgatggggat
    19681 attttggcat ttgatcggcg cggccagtgc agcctgtttt tacgctccgt tcaaaaaagt
    19741 aaaaaaatgg tcatgggaaa ccatgtggtc agtcggtggg attgtttcgt ggattattct
    19801 gccgtgggcc atcagcgccc tgttactacc gaatttctgg gcgtattaca gctcgtttag
    19861 tctctctacg ctgctgcctg tttttctgtt cggcgctatg tgggggatcg gtaatatcaa
    19921 ctacggcctg accatgcgtt atctcggcat gtcgatggga attggcatcg ccattggcat
    19981 tacgttgatt gtcggtacgc tgatgacgcc aattatcaac ggcaatttcg atgtgttgat
    20041 tagcaccgaa ggcggacgca tgacgttgct cggcgttctg gtggcgctga ttggcgtagg
    20101 gattgtaact cgagccgggc agttgaaaga gcgcaagatg ggcattaaag ccgaagagtt
    20161 caatctgaaa aaagggctgg tgctggcggt gatgtgcggc attttctctg ccgggatgtc
    20221 ctttgcgatg aacgccgcaa aaccgatgca tgaagccgct gccgcacttg gcgtcgatcc
    20281 actgtatgtc gctctgccaa gctatgttgt catcatgggc ggcggcgcga tcatcaacct
    20341 cggtttctgt tttattcgtc tggcaaaagt gaaggatttg tcgctaaaag ccgacttctc
    20401 gctggcaaaa ctgctgatca ttcacaatgt gttactctcg gcactgggcg ggttgatgtg
    20461 gtatctgcaa ttctttttct atgcctgggg ccacgcccgc attccggcgc agtatgacta
    20521 catcagttgg atgctgcata tgagtttcta tgtattgtgc ggcggtatcg tcgggctggt
    20581 gctgaaagag tggaacaatg caggacgccg tccggtaacg gtgttgagcc tcggttgtgt
    20641 ggtgattatt gtcgccgcta acatcgtcgg catcggcatg gcgaattaat ctttctgcga
    20701 attgagatga cgccactggc tgggcgtcat cccggtttcc cgggtaaaca ccaccgaaaa
    20761 atagttacta tcttcaaagc cacattcggt cgaaatatca ctgattaaca ggcggctatg
    20821 ctggagaaga tattgcgcat gacacactct gacctgccgc agatattgat tgatggtcat
    20881 tccagtctgc tggcgaaatt gctgacgcaa aacgcgctca ctgcacgatg cctcatcaca
    20941 aaatttatcc agcgcaaagg gacttttcag gctggccgcc agccgggtaa tcagcttatc
    21001 cagcaacgtt tcgctggatg ttggcggcaa cgaatcactg gtgtaacgat ggcgattcag
    21061 caacatcacc aactgcccga acagcaactc tgccatttcg ttagcaaacg gcacatgctg
    21121 actactttca tgctcaagct gaccgataac ctgccgcgcc tgtgccatcc ccatgctacc
    21181 taagcgccag tgtggttgcc ctgcgctggc gctaaatccc ggaatcgccc cctgccagtc
    21241 aagattcagc ttcagccgct ccgggcaata aataatattc tgcaaaacca gatcgttaac
    21301 ggaagcgtag gagtgtttat cgtcagcatg aatgtaaaag agatcgccac gggtaatgcg
    21361 ataagggcga tcgttgagta catgcagacc attaccgcgc cagacaatca ccagctcaca
    21421 aaaatcatgt gtatgttcag caaagacatc ttgcggataa cggtcagcca cagcgactgc
    21481 ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt aactgatgcg ccaccgtggc
    21541 tacctcggcc agagaacgaa gttgattatt cgcaatatgg cgtacaaata cgttgagaag
    21601 attcgcgtta ttgcagaaaa ccatcccgtc cctgacgaat atcacgcggt gaccagttaa
    21661 actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct gaatccgcag cgataggcga
    21721 tgtcagtaac gctggcctcg ctgtggcgta gcagatgtcg ggctttcatc agtcgcaggc
    21781 ggttcaggta tcgctgaggc gtcagtcccg tttgctgctt aagctgccga tgtagcgtac
    21841 gcagcgaaag agaaaattga tccgccacgg catcccaatt cacctcatcg gcaaaatggt
    21901 cctccagcca ggccagaagc aagttgagac gtgatgcgct gttttccagg ttctcctgca
    21961 aactgctttt acgcagcaag agcagtaatt gcataaacaa gatctcgcga ctggcggtcg
    22021 agggtaaatc attttcccct tcctgctgtt ccatctgtgc aaccagctgt cgcacctgct
    22081 gcaatacgct gtggttaacg cgccagtgag acggatactg cccatccagc tcttgtggca
    22141 gcaactgatt cagcccggcg agaaactgaa atcgatccgg cgagcgatac agcacattag
    22201 tcagacacag attatcggta tgttcataca gatgccgatc atggtcgcgc acaaaacata
    22261 ccgtgccacc ggtgatggta tagggctgcc cattaaacac atgaataccc gtgccatgtt
    22321 cgacaatcac aatttcatga aaatcatgat gatgttcagg aaaatccgcc tgcgggagcc
    22381 ggggttctat cgccacggac gcgttaccag acggaaaaaa atccacacta tgtaatacgg
    22441 tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt aatcacgagg tcaggttctt
    22501 accttaaatt ttcgacggaa aaccacgtaa aaaacgtcga tttttcaaga tacagagtga
    22561 attttcagga aatgcggtga gcatcacatc accacaattc agcaaattgt gaacatcatc
    22621 acgttcatct ttccctggtt gccaatggcc cattttcctg tcagtaacga gaaggtcgcg
    22681 aattcaggcg ctttttagac tggtcgtaat gaaattcagc aggatcacat tatgaccttt
    22741 cgcaattgtg tcgccgtcga tctcggcgca tccagtgggc gcgtgatgct ggcgcgttac
    22801 gagcgtgaat gccgcagcct gacgctgcgc gaaatccatc gttttaacaa tgggctgcat
    22861 agtcagaacg gctatgtcac ctgggatgtg gatagccttg aaagtgccat tcgccttgga
    22921 ttaaacaagg tgtgcgagga agggattcgt atcgatagca ttgggattga tacctggggc
    22981 gtggactttg tgctgctcga ccaacagggt cagcgtgtgg gcctgcccgt tgcttatcgc
    23041 gatagccgca ccactggcct aatggctcag gcacaacaac aactcggcaa acgcgatatt
    23101 tatcaacgta gcggcatcca gtttctgccc ttcaatacga tctatcagct acgtgcactg
    23161 acagagcagc aaccagaact tattccacac attgctcacg ctttgctgat accggattac
    23221 tttagttatc gcctgaccgg caagatgaac tgggaatata ccaatgccac aaccacgcaa
    23281 ctggtcaata tcaatagcga cgactgggac gagtcgctac tggcgtggag cggtgccaac
    23341 aaagcctggt ttggtcgccc gacgcatccg ggtaatgtca taggccactg gatttgcccg
    23401 cagggtaatg agattccggt ggtcgccgtt gccagccatg ataccgccag cgcggttatc
    23461 gcctcgccgt taaacggttc acgtgctgct tatctctctt ctggcacctg gtcattgatg
    23521 ggttttgaaa gccagacgcc atttaccaat gacacggcac tggcagccaa catcaccaat
    23581 gaaggcgggg cggaaggtcg ctatcgggtg ctgaaaaata ttatgggctt atggctgctt
    23641 cagcgagtgc ttcaggagcg gcaaatcaac gatcttccgg cgcttatcgc cgcgacacag
    23701 gcacttccgg cttgccgctt cattatcaat cccaatgacg atcgctttat taatcctgag
    23761 acgatgtgca gcgaaattca agctgcgtgt cgggaaacgg cgcaaccgat cccggaaagt
    23821 gatgctgaac tggcgcgctg cattttcgac agtctggcgc tgctgtatgc cgatgtgttg
    23881 catgagctgg cgcagctgcg cggtgaagat ttctcgcaac tgcatattgt cggcggaggc
    23941 tgccagaaca cgctgctcaa ccagctatgc gccgatgcct gcggtattcg ggtgatcgcc
    24001 gggcctgttg aagcctcgac gctcggcaat atcggcatcc agttaatgac gctggatgaa
    24061 ctcaacaatg tggatgattt ccgtcaggtc gtcagcacca ccgcgaatct gaccaccttt
    24121 acccctaatc ctgacagtga aattgcccac tatgtggcgc agattcactc tacacgacag
    24181 acaaaggagc tttgcgcatg accactcaac tggaacaggc ctgggagctg gcgaaacagc
    24241 gtttcgtggc ggtggggatc gatgtcgagg aggcgctgcg ccaacttgat cgtttacccg
    24301 tttcaatgca ctgctggcag ggtgatgatg tttccggttt tgaaaacccg gaaggttcgc
    24361 tgaccggtgg gattcaggct actggtaatt atccgggcaa agcgcgtaat gccagtgagc
    24421 tacgagccga tctggaacag gctatgcggc tgattccggg gccgaaacgg cttaatttac
    24481 atgccatcta tctggaatca gacacgcctg tctcgcgtga ccagatcaaa cctgagcact
    24541 tcaaaaactg ggttgaatgg gcgaaagcca atcagctcgg tctggatttt aacccctcct
    24601 gtttttcgca tccgctaagc gccgatggct ttacgctttc ccatgccgac gacagcattc
    24661 gccagttctg gattgatcac tgcaaagcca gccgtcgcgt ttcggcctat tttggcgagc
    24721 aactcggcac accatcggtg atgaacatct ggatcccgga tggtatgaaa gatatcaccg
    24781 ttgaccgtct cgccccgcgt cagcgtctgc tggcagcact ggatgaggtg atcagcgaga
    24841 agctaaaccc tgcgcaccat atcgacgccg ttgagagcaa attgtttggc attggcgcgg
    24901 agagctacac ggttggctcc aatgagtttt acctggggta tgccaccagc cgccagacgg
    24961 cgctgtgcct ggacgccggg catttccacc cgactgaagt gatttccgac aagatttccg
    25021 ccgccatgct gtatgtgccg cagttgctgc tgcacgtcag ccgtccggtt cgctgggaca
    25081 gcgatcacgt agtgctgctg gatgatgaaa cccaggcgat tgccagtgag attgttcgtc
    25141 acgatctgtt cgaccgggtg catatcggcc tcgacttctt tgatgcctct atcaaccgca
    25201 ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc cctgctacgt gcgttactgg
    25261 aacctaccgc tgaactacgc aagctggaag tggcgggcga ttacactgcg cgtctggcgc
    25321 tactggaaga acagaaatcg ttgccgtggc aggcggtatg ggaaatgtat tgtcagcgtc
    25381 acgatacgcc agcgggtagc gaatggctgg agagcgtgcg ggcttatgag aaagaaattt
    25441 tgagtcgccg cgggtaaaca ctgccggatg cggcacaagt gccttatccg gcctacaggt
    25501 cggcaacagt tgtaggcctg ataagacgcg ac
//
LOCUS       DAZUCE010000052        25273 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZUCE010000052 DAZUCE010000000
VERSION     DAZUCE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.052"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..187)
                     /locus_tag="ACHY03_001813"
     CDS             complement(<1..187)
                     /locus_tag="ACHY03_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HFS3905052.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSL"
     gene            complement(261..1205)
                     /gene="yfdV"
                     /locus_tag="ACHY03_001814"
     CDS             complement(261..1205)
                     /gene="yfdV"
                     /locus_tag="ACHY03_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFS3905053.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(1275..2969)
                     /gene="oxc"
                     /locus_tag="ACHY03_001815"
     CDS             complement(1275..2969)
                     /gene="oxc"
                     /locus_tag="ACHY03_001815"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFS3905054.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVASAISLLAKAERPLVIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(3023..4273)
                     /gene="frc"
                     /locus_tag="ACHY03_001816"
     CDS             complement(3023..4273)
                     /gene="frc"
                     /locus_tag="ACHY03_001816"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFS3905055.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSDFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(4785..5417)
                     /gene="yfdX"
                     /locus_tag="ACHY03_001817"
     CDS             complement(4785..5417)
                     /gene="yfdX"
                     /locus_tag="ACHY03_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFS3905056.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            5713..5988
                     /gene="ypdI"
                     /locus_tag="ACHY03_001818"
     CDS             5713..5988
                     /gene="ypdI"
                     /locus_tag="ACHY03_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFS3905057.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGGSERSYKETEKTSAM
                     TTTHSTKIQPSQAILFKMREDAPPLNLTEETTPPFPTKANYLIHPVR"
     gene            complement(6065..6307)
                     /gene="yfdY"
                     /locus_tag="ACHY03_001819"
     CDS             complement(6065..6307)
                     /gene="yfdY"
                     /locus_tag="ACHY03_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFS3905058.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            6660..7580
                     /gene="lpxP"
                     /locus_tag="ACHY03_001820"
     CDS             6660..7580
                     /gene="lpxP"
                     /locus_tag="ACHY03_001820"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFS3905059.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            7936..8007
                     /gene="ypdK"
                     /locus_tag="ACHY03_001821"
     CDS             7936..8007
                     /gene="ypdK"
                     /locus_tag="ACHY03_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFS3905060.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(8072..9310)
                     /gene="alaC"
                     /locus_tag="ACHY03_001822"
     CDS             complement(8072..9310)
                     /gene="alaC"
                     /locus_tag="ACHY03_001822"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFS3905061.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            9687..11384
                     /gene="ypdA"
                     /locus_tag="ACHY03_001823"
     CDS             9687..11384
                     /gene="ypdA"
                     /locus_tag="ACHY03_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFS3905062.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            11399..12133
                     /gene="ypdB"
                     /locus_tag="ACHY03_001824"
     CDS             11399..12133
                     /gene="ypdB"
                     /locus_tag="ACHY03_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFS3905063.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            12146..13003
                     /gene="ypdC"
                     /locus_tag="ACHY03_001825"
     CDS             12146..13003
                     /gene="ypdC"
                     /locus_tag="ACHY03_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3905064.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(13006..15501)
                     /gene="ptsP"
                     /locus_tag="ACHY03_001826"
     CDS             complement(13006..15501)
                     /gene="ptsP"
                     /locus_tag="ACHY03_001826"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFS3905065.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASTSDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLTYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKTIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(15526..16563)
                     /gene="ypdE"
                     /locus_tag="ACHY03_001827"
     CDS             complement(15526..16563)
                     /gene="ypdE"
                     /locus_tag="ACHY03_001827"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS3905066.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMLCAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTTWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(16563..17648)
                     /gene="ypdF"
                     /locus_tag="ACHY03_001828"
     CDS             complement(16563..17648)
                     /gene="ypdF"
                     /locus_tag="ACHY03_001828"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS3905067.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATYTLTTIARQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(17663..18910)
                     /gene="fryC"
                     /locus_tag="ACHY03_001829"
     CDS             complement(17663..18910)
                     /gene="fryC"
                     /locus_tag="ACHY03_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFS3905068.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKTSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFSAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(18932..19258)
                     /gene="fryB"
                     /locus_tag="ACHY03_001830"
     CDS             complement(18932..19258)
                     /gene="fryB"
                     /locus_tag="ACHY03_001830"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFS3905069.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(19477..20442)
                     /gene="glk"
                     /locus_tag="ACHY03_001831"
     CDS             complement(19477..20442)
                     /gene="glk"
                     /locus_tag="ACHY03_001831"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFS3905070.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            20646..21902
                     /gene="yfeO"
                     /locus_tag="ACHY03_001832"
     CDS             20646..21902
                     /gene="yfeO"
                     /locus_tag="ACHY03_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFS3905071.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLTALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            22017..22343
                     /gene="ypeC"
                     /locus_tag="ACHY03_001833"
     CDS             22017..22343
                     /gene="ypeC"
                     /locus_tag="ACHY03_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFS3905072.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(22473..23711)
                     /gene="mntH"
                     /locus_tag="ACHY03_001834"
     CDS             complement(22473..23711)
                     /gene="mntH"
                     /locus_tag="ACHY03_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFS3905073.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            24047..25249
                     /gene="nupC"
                     /locus_tag="ACHY03_001835"
     CDS             24047..25249
                     /gene="nupC"
                     /locus_tag="ACHY03_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFS3905074.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         6328 a         6538 c         6088 g         6319 t
ORIGIN      
        1 agagtgactg tccatccaca tagggaccaa atgtgcgggt atcatcacca tgacctggtg
       61 gctcaacttt aattaccctt gcgcccatat tacaaagaag ttgagttccg aaaggtccat
      121 taaggacatg tgtcatatcg ataactaata agccttcaaa cggccctttg ctttcattat
      181 ttgtcatttg cttatgcctt gaaaaaagcc gaaggtgaaa agacacttcg gcatttatgt
      241 aaaatcaata acatgattaa ttatgaaacc agtcgactga cataaatcca caagggagcc
      301 gtgacgacaa aacccagtac gctcacagcc aatgacgctg taccagtgcg ggtgtagaca
      361 ttaaaccggc tggcaataat gattccggag aatgccggcg gtaatgcgcc tgccagtacc
      421 atcatctgca gatgttcgct gttcaaatga catgccatac caacaagaag cagcgccagt
      481 ggcatcagaa tcagcttcag gaaggtgtta taagcaattt cggcactgaa ctcgaattta
      541 tgtgcagcca gagtcaaccc cgcagcgaat accgctaccc ctgagttagc cttcgcaatc
      601 agattaaagg ttgggtccca tgctgccgga atttttaccc caaccagcac caggatcgtt
      661 gccagaacag gtgcccatac tacaggctcc tttgccgcag aaattaatgc gctcagatta
      721 ctattcttct taccatccgc tcctgaggaa ggattcagca aatacagacc aataggaata
      781 gtaattgcgt taacaataat agaaataatt gctaccacta aacctgttga tacggaatca
      841 ccataaatag gatcgagaac tgcaaatccc aggaatccaa tggtaggtga acctgcaatt
      901 aatgcacata cagctgcttc tgcatgggta cgtttaaaaa atttgtagca accaaaccag
      961 gagaaaaaga aacatccgac aataaccaca agtgatacaa gggtcagacg agtgtccgca
     1021 aaaatcattt ccctgtttgc ccgggtaata gaaacaaata gagccgcagg aagagcgtag
     1081 tttaatacca gtttattaaa tgcccgggct tgatcttctg aaaaagtttc tcgtctgccg
     1141 ctaaaataac ccaacagcat gattacgata ataggcaata aatcgccaat aaaaaatgtt
     1201 agcataatag tatttccgat aagggtcccc tcaccttcgc ataatgagtt ggcaagagga
     1261 gacgatgtca gtatttaatt accagcgact tgttttgggt taagtttggt aatatggcca
     1321 ctttcagttc ctgctgcagg gtcgatgacc acattaataa tggtcggttt gcgcgactga
     1381 ataccggtgg ttaaagcatg acgaagttca tctgtcgtgg tgacgttata gccaacgcca
     1441 cgaaacgcat ccattaattt gtcatacctt gcatggtgca acagatctgt tggtgatggt
     1501 gcaccagcgc cactgagatc aacaccgtct cctctgtaga tgccgccatt attaaaaata
     1561 acgatcgtca ccggcaggtt atatcgacaa atcgtttcaa tctccatccc actgaaacca
     1621 aaagcactat caccttcaat ggcgacaacc ggagaaccag aggtcacgct agcaccgatg
     1681 gcatagccca taccgatgcc catgacaccc caggtaccac aatccagacg acgacgtggt
     1741 ttatacatat caataatatt tcgtgcatta tccagggtat ttgcaccttc attaactaaa
     1801 taaatatcct ggttctcacg caatacatcg cgcacagcac ttaatgcatt aaagtaattt
     1861 aatggttggg tatctgtact taatttttca tgcatttttt gtgcattttg ctgcttgtgg
     1921 atatttaaaa tatcgcgcca taccagtgga gtcgtaaatg tgttttgttt cagctctgcc
     1981 aacatacctt gcatactgga tgcaatatcg ccaacgactg gcacagcaat agggcggttg
     2041 ctgtcaattt cctgcggttc aatatccagt tgaataaact gtgtatctgc cgcccatcct
     2101 tttttaccgt gtgccagtaa ccaattcagt cgtgcaccaa caagcatgac aacgtcagca
     2161 tttgccaggg caaacgaacg cgcagctgcc gcagaaagtg gatgcgtatc ttcgaggatc
     2221 cctttcgcca tagacattgg caggaatgga atctgggcac tttcaataaa ttcacgaagc
     2281 tgttcgtcag cttgtgaata cgccgcgcct ttgccaagga taactaatgg tcgttcagct
     2341 tttgctaaaa gcgaaattgc gctagcgact gacttcgggc atggcaataa tgctggcgac
     2401 ggattttcaa cttttacaat cgtggttaac gcttcgtctt tttccatcgt cgcggccagg
     2461 acatttgctg gcaaatcaag ataaactccg ccagggcgac ccgatacgga gacccggata
     2521 gcgcgtgcca atgcaatgcc aagatcctgc ggctgattaa cgcgaaatgc tgctttggca
     2581 tacggttttg ccgcattcat ttggtccagc tcttcataat caccttgctg taggtcgacg
     2641 atcgcgcggt cgctggagcc gctaatcatg atcatcggaa aaccatttac cgttgcgttg
     2701 gccaatgcgg tcaaaccatt gaggaatcct ggcgcagaaa ctgtcaggca gatccccggt
     2761 ttttgggtaa gaaaaccgct tgccgcagcg gcatagcctg ccgactgctc atgacgaaaa
     2821 ccaatataac gaatgccttc cgcctgggca tggcgcgcca tatccgtcac aggaatacct
     2881 acaacaccat aaatagtgtc aatattattc tgttttaatg cttcaacgat gatatgcata
     2941 ccatctgtca tttgaagttg atctgacatg attaataacc tcgaaatttt tataaattgg
     3001 gagccagaag cccccgttaa tatcagatgg cgtggttttg cttcattgca gcaatttcat
     3061 cgtcgctata acccagctcc tgcaatacag cagcggtatg ttcacctaat agcggcgcag
     3121 ctttaatatc cggcgtaaag tcagagaatt tcattggaca accaactgtc agatattttc
     3181 cacgcaacgg ttgttccact tcgacaacac tgccactttg gcgcaaagag ggatcaagtg
     3241 aaatttcttt catacttaaa accggtgcac aaggaatatc aaattgagtc aaataggcca
     3301 ccgcttcatg tttatcaata gtgacagtgt atttttcgat ttcagcaaaa atatcgaaaa
     3361 tatgtggctg tcgggcatgg gctgtactgt atgccggatc ggtaatccaa tctggtttgc
     3421 cgatggcttt acaggtgttt tcccagtttt gctcctgaat agtgaaataa atataggcgt
     3481 taggatcggt ttcccagcct ttacatttca ggatccagcc aggctgaccg ccaccacccg
     3541 cattacctcc acggggaact gcatcaccaa atgtgccatt cggatactgc gggtattctt
     3601 ccagataacc caatttatcg agacgctgct ggtcgcgtaa tttcacgcgg caaaggttca
     3661 atacggcatc ctgcattgac atggtgactc gttgcccacg ccccgttttt tcgcgatgca
     3721 gcaaagcagc aagtaaaccg atcagtaaat gcattccggt gttgctgtca cccaacgctg
     3781 cagcgcttac cagcggcgga ccgtcccaaa aacccgtagt ggatgccgcg ccacccgctg
     3841 cctgagcaac gttttcatag gcttttacat tcacataagg cgaacactca tcaaaccctt
     3901 tgatcgaacc aaaaatcaga cgtggattga tttcttgaat atgctcccag gtgaaaccca
     3961 tgtgatcaat ggcccctgga tgaaagttct cgactaagat atcagcttcg cggatcagct
     4021 tttccattac ctctttgcct tccgctgttt tggtatttaa ctcaatagaa cgtttgttac
     4081 tgttaagcat ggtgaagtaa agcgcatcga tatcaggaat atctcgcagc tggtgacgcg
     4141 ttacgtcacc aacgccgggg cgttcaattt taatgacgtc agcgccaaac caggccagca
     4201 tttgagtaca agatgggcca gattgcacac cggtgaaatc gagaacttta attccttgaa
     4261 gtggagttga catagcaata cctattagtt aattaaatat gtgttaattt agcgtttatt
     4321 taaggcataa cgcctctata aataatccgc aaaatatcgt cgtagctgca ctcgatattt
     4381 ttacattcct gtatcaagta ctgaaaatca tttcatcgct acttccaatt cctttctgca
     4441 tggtaagtag tgaggacgaa aagaagaata tgataaatat tggttcgtgg taatcagaga
     4501 gagtcgcgac atttgtgatg ttattcttac aactgtaaga agcgccggtg atttacaagt
     4561 aagggtatga ttacgctttt taaagcgtac cataaaatat aacatattaa atgatttgtg
     4621 atgacacggg attattgcgg tttactttga ggaatataaa atgcgagttt atataaccgc
     4681 gagcgccaaa taattttatg accacaacct gtcgtattgt ggtcataaaa aattgtcggc
     4741 tggagggagt taaatccttt acctcaatat actgcctgtc agcattagtt cacaaacagt
     4801 gcttcgctat cgacgatgat tccatcttcc gcacctttaa gcgccaggtt tgcctcgtag
     4861 tattgctttt tatcaagcag tttctgcgca tcagcaagtg cattacgagt ctgtttaagt
     4921 ggcatcagat attggttttc cattacgcca acaccggcca ggcgaagctc ttccattgcg
     4981 ccctttttat cccctttcgc cattttttca ttggcaattt ttatcgcggc ttctttttcg
     5041 ggtgttgcta cataactctc agaaattccg acagatgcat taatgacaat atattggtca
     5101 tcattgagat tggtcttttt acctggctta gcgaactttg cccattcggt actgtcatct
     5161 gacaacaaag cggaagcttc attggtcagt tcttttgcct tttccggatc gccatgaaac
     5221 agagccaggc gcgctacctg cacgtcacgc atcgcatata aaccttgttc agagatacgc
     5281 tctgctgctg ccgttttctg ggtttgctga acttgctgag tttgctgttg tgccgctacc
     5341 ggtgcattat cagcagccca tacagttgaa gatgccagaa tagctgtgac catcgtagcc
     5401 ataattaaac gtttcatact tacctcattc tttaacagtt ttagaaaacg ccgccatcat
     5461 attgggtgag catgaaatga gaaatcagag agcaacggtc attttgaaag ggttatctta
     5521 cagttgtagt tattatcgca agaagtattt tagtcttaca attgtaggaa tatgcttcct
     5581 gtcacagaga actattctta tgtaccacaa agagatgtca tttattatat cgatgtaaat
     5641 attgattaat tatatgtaaa tacagccata acatcgtaca ttctcagtga accattttca
     5701 ggataacttt ctttgaaagt aaacttaata ctttttagct tatttttatt ggtctctatt
     5761 atggcatgca atgtttttgc attttccatt tctggtggtg gaagtgagag gagctataaa
     5821 gagacggaaa aaacatcagc gatgacgaca actcactcta caaaaattca gccatcacag
     5881 gcaattttgt ttaagatgag agaagatgcg ccaccattaa acctcacaga agaaaccacg
     5941 cccccttttc cgacaaaggc gaattatctt attcatcctg tgcgatagcg tctgtcacct
     6001 ggcaggtgaa caatcatgtc tgatgctgat gtgaacacct gcccaaatga gccatcctga
     6061 cccactatcg cctgccatac cccaccataa agcgaatcat caagccacaa gccggggcaa
     6121 gaaacataaa tatccccgtc accagttcat taccgccagt taaccacatg gtgaagtaat
     6181 aaatgagtgc agcgaggatc accatgccga agaaagaaga gaccgccgat accacgttca
     6241 gtacctgacc gatgtatccg cttacgcaga caatacacag ggcgagaaac atccataaat
     6301 aaatcatagc tcaacctcct gccgcgctaa aaaacctgta acttcagtca ctttaatagt
     6361 aagacaatcc ttactctctg gcaatttttg ctaagcatca atgaaaacag catgttaaat
     6421 gcaagactgt tgtgtacgga aaaatattta ctttgcacga ttaataatca gtagctgaaa
     6481 gcagtcagcg agtgataaag tacagatacc gcattcatgc tcctcgtggt tatgtcctga
     6541 ctagtcttta cactctttac aggaaccatt gtcgtacatg atggcctaat caattgagtg
     6601 taccgctgtc gacatggaat cttctgcgat acaacaattc gtatctacag aaggtaacta
     6661 tgtttccaca atgcaaattt tcccgcgagt ttctacatcc tcgctactgg ctcacatggt
     6721 ttgggcttgg tgtactctgg ctttgggtac agcttcctta tcctgttctc tgctttctcg
     6781 gcacgcgtat tggcgcaatg gcgcgaccat tcctgaaacg gcgtgaatct atcgcccgta
     6841 aaaacctgga actttgcttc ccgcagcatt ctgcggaaga acgcgagaag atgattgccg
     6901 aaaactttcg ttcactcggc atggcgctgg tagaaaccgg catggcgtgg ttctggcccg
     6961 acagtcgcgt acgtaaatgg tttgatgttg aagggctgga taaccttaaa cgcgcacaaa
     7021 tgcaaaatcg cggcgtaatg gttgtcggcg tccattttat gtcgctggaa ctgggcggcc
     7081 gcgtgatggg actttgtcag ccaatgatgg ctacctatcg tccacataat aatcagctga
     7141 tggaatgggt gcagacccgt ggtcgcatgc gctctaacaa agcgatgatc ggcagaaata
     7201 atctgcgcgg cattgtcagt gcactgaaga aaggtgaagc ggtatggttt gctcccgatc
     7261 aggattatgg tcgtaaaggc agctccttcg cgccgttctt tgcggtagaa aatgtcgcca
     7321 caaccaatgg cacctatgtt ctctcccgtc tctctggcgc agccatgttg accgtaacga
     7381 tggtaagaaa agcggattac agcggatatc gtttgttcat caccccagag atggaaggct
     7441 acccgacaga tgaaaatcaa gccgctgcct atatgaacaa gattatcgag aaagagatca
     7501 tgcgcgcacc ggagcagtac ctctggatcc accgtcgctt taaaacgcgc ccggtgggag
     7561 aatcgtcgtt atacatttaa caaactgctc tgactcttta cctcctcaca taagccataa
     7621 taacctcatg gtttttatgg cttttttctt aaggtttttt ccttcgctga atctctaatt
     7681 attaccgtca gaaattaaac tgtcgccgat atgagacacc tgtaagttac taaaagtaaa
     7741 taagaaaatg cctcttgtct cgtttcagtt ttaggcgtac attagcgccg tctcgagcag
     7801 tttagctcag aattctgggg tgatgcccac ggtaaaatac aggactttta aggtcgtttt
     7861 gcccggtgtc aactcactgt atcaggtgta atgaagtcat tcaggcgtaa cagcaattac
     7921 gcggagagat gtaaagtgaa atatttcttt atgggcattt cttttatggt catcgtttgg
     7981 gccggtactt ttgccctgat gatctaaaag caaactgtgc aatgaaaaac aggagccatc
     8041 aggctcctgt ttgcatttct gaggtcggtg tttattccgc gttttcgtga atatgtttgc
     8101 tgctggcggg tagtaaaccg tcggcacgga acatcgcttt aatcccacga atcgcctgac
     8161 gaatacggtc gcggttttca atcagtgcaa agcgcacatg cgtatcaccg tagtcgccaa
     8221 agccaatccc cggagagaca cagaccttcg cttcgttaag caatttcttg gcaaattcca
     8281 gcgagcccat ggcagcatac ggttccggta ttttcgccca gacatacatc gaagccttcg
     8341 gcatttcgac catccagccc gcttcatgca gccctttaac caatacatca cggcggcgtt
     8401 tgtactgttc ggcaatgtcg cgcacgcact gttgatcgcc ctcgagtgcc gcaatcgccg
     8461 ccacctgcaa cggcgtaaag gtgccgtaat cgtgatagct tttaatacgt gccagggcgc
     8521 tgaccagcgt tttgttgcca accataaagc cgatacgcca gcccgccatg ttgtagcttt
     8581 tcgacagcgt aaagaactcg accgccacat cgcgtgcacc tggcacctgc atgattgacg
     8641 gcgctttcca gccatcgtag acgatgtccg cataggccag gtcatggacc accagcacat
     8701 cgtagcgttt cgccagcgcc acaacctttt cgaagaactc cagttccacg cattgcgcgg
     8761 ttgggttcga cgggaagccg aggatcatca tcttcggttt cggataactt tcgcgaatgg
     8821 cgcgctccag ttcattgaag aaatcgacac cttccaccag cggcactgag cgtacctgag
     8881 ccccggcaat caccgcgccg taaatatgaa tggggtaact tggattcggc accagcactg
     8941 tgtcaccgtg atccagtgtc gccagcatca gatgcgccag gccctctttc gaaccaatag
     9001 tgacgatggc ttctgattcc gggtcgattt caacgtcgta gcgatcctga taccagcggg
     9061 aaatggcgcg acgtagccgc ggaataccgc gtgaagtgga gtaaccatgc gtgtccgggc
     9121 gctgggccac agtacataat ttttcgacga tatgcggcgg agtcgcaccg tccgggttac
     9181 ccatactgaa atcgataata tcttcgccgc gccgacgcgc agccattttc agttcagcgg
     9241 taatgttaaa aacatagggc gggagacgat caatgcgcgt aaagcgacgt tcagggcgag
     9301 tgtcagccat aatttcctca gattaacgtt agcgcccgga ccgtccgagc gacgctgcca
     9361 cgatggtggc tgttttgaaa atagcctgat taatttctgt ctgtccagcg ttttagaaca
     9421 tttttattaa tgacgttaaa tgaggaactc tgctttaaaa acaggaaatt tatctgcgct
     9481 aacaagaaaa ttaccactca ttttattaac ataataattt caataaattt actaaccaga
     9541 agtgcattat caagagatgc attgctaata caccatttat ctttcaatcc ccttttaaaa
     9601 ttgtggtttt tcctcaaaaa cggcctgctg gtcatcagcc ttcaggttac ctatcataga
     9661 ggtttaatcc ttattcagag tcacccgtgc acgaaatatt caacatgctg ctggcggtct
     9721 tcgatcgggc agcgttaatg cttatctgcc tgttctttct catccgtatc cgcctgtttc
     9781 gcgaactgtt gcacaagtcg gcacactcac cgaaagagtt gctcgccgtt accgccattt
     9841 tctcgctgtt cgccctgttc agcacctggt ccggcgttcc cgtagaaggc tcgctggtga
     9901 acgtacgtat tatcgcggtg atgtccggcg ggattctgtt tggcccgtgg gtgggcatca
     9961 ttaccggcgt gattgcgggt attcatcgtt atttaattga tatcggcggc gtgacggcga
    10021 tcccctgctt tatcaccagc attctggctg gttgtatatc gggctggatc aacctgaaaa
    10081 tccccaaagc acagcgctgg cgcgtcggta ttctcggcgg catgttgtgt gaaacgttga
    10141 ccatgattct ggtgattgtc tgggcaccaa ctaccgcgtt ggggatcgat atcgtctcta
    10201 aaatcgggat tccaatgatc ctcggcagcg tctgtatcgg ctttattgta cttctggtgc
    10261 aaagcgttga gggcgaaaaa gaggccagcg ccgcgcggca ggccaagttg gcgctggata
    10321 tcgccaacaa aacgctacct ctgtttcgcc atgtcaatag cgagtcatta cgcaaagttt
    10381 gtgaaattat tcgcgatgac attcacgccg atgcggtggc gattaccaat accgatcatg
    10441 tgctggccta tgttggcgtg ggtgaacata actatcagaa tggcgatgac ttcattagcc
    10501 cgactacccg tcaggcgatg aattacggaa aaatcatcat taaaaacaat gatgaagccc
    10561 accgcacacc agagattcat tccatgctgg tgatcccatt gtgggagaaa ggcgttgtga
    10621 ccggaacgct gaaaatttac tactgccacg cgcatcagat aacctcgtca ttacaggaaa
    10681 tggcggtcgg tctatcgcaa atcatctcca cgcaactgga ggtttcacgc gccgagcagc
    10741 tacgtgaaat ggcaaataag gcagagcttc gcgccctgca aagcaaaatt aatccccatt
    10801 ttctgtttaa cgctctgaac gctatttcat cgtcaatccg tctgaatccg gatactgctc
    10861 gccagctgat ctttaatctg tcgcgttatc tgcgctataa cattgaatta aaagacgatg
    10921 agcaaatcga tatcaaaaaa gagctgtatc aaattaaaga ctatattgcc attgagcagg
    10981 cccgctttgg cgacaagctg acggttatct atgatattga tgaagaggtg aattgctgca
    11041 ttcccagcct gctgatccag ccgttggtgg agaatgccat tgtccacggt atccagcctt
    11101 gcaaaggtaa aggcgttgtc accatcagcg ttgcagagtg cggcaatcgg gtacgcattg
    11161 cggtgcggga taccgggcac ggcatcgatc caaaggtgat tgagcgggtc gaagcgaatg
    11221 aaatgccggg caataaaatt ggcctgctga atgtccatca tcgcgtgaag ttattgtatg
    11281 gcgaggggct gcatatccgc cgcctggaac caggaacgga aattgcgttt tacattccta
    11341 accaacgcac cccagtcgcc tcacaggcta cgttattgct ttgagccgga gtgatattgt
    11401 gaaagtcatc attgttgaag acgaattcct ggcacaacag gaactgagct ggctaattaa
    11461 agagcacagc cagatggaga ttgtcggcac ctttgacgac ggtctggacg tgttgaagtt
    11521 tttgcagcat aaccgcgtcg acgccatttt tctggatatc aatattccgt cgctggatgg
    11581 tgtgttgctg gcacaaaaca tcagccagtt cgcccataaa ccgtttattg tgttcatcac
    11641 cgcgtggaaa gaacatgcgg tagaagcgtt tgaactggag gcgtttgact acattctaaa
    11701 accgtatcag gagtcacgca ttaccgggat gctgcaaaaa ctggaagcag cctggcaaca
    11761 acagcagacc agcagcacgc ctgccgcgac ggtaacgcgt gagaatgaca ccattaatct
    11821 ggtgaaagat gagcgaataa tcgtcacgcc aattaacgat atctattacg ccgaagcgca
    11881 cgagaaaatg acctttgttt atacgcggcg tgaatcctac gtaatgccga tgaacattac
    11941 cgaattttgc agcaaactgc cgccgtcgca ttttttccgc tgccatcgct cattttgtgt
    12001 caatctgaac aaaatccgcg aaatcgaacc gtggtttaat aacacctata ttctgcggct
    12061 gaaagatctg gattttgaag tgccggtcag ccgcagcaaa gttaaagaat tccgccagtt
    12121 aatgcatctt taatcgggaa ctttcatgaa agcaccaggc ttgcccgccg atcaacaatt
    12181 tttcgccgat ctgttcagcg gcctggtgct taacccgcaa ctactggggc gcgtctggtt
    12241 tgccagccag cctgcctcgt tgccggtggg cagtttatgt attgattttc cccgtctgga
    12301 tatcgtgctg cgcggcgaat acggcaatct gctggaagca aagcagcaac gtttggtgga
    12361 aggagaaatg ctgtttattc cggcgcgcgc ggctaattta ccggtcaaca acaaaccggt
    12421 gatgctgtta agcctggtgt tcgctccgac ctggcttggg ttatcgtttt acgatagccg
    12481 caccacgtcg ttgttgcatc ctgctcgcca gatccagctt cccagcctgc aacgcggtga
    12541 aggtgaagcg atgcttaccg ccctcaccca tcttagccgt tcgccgctgg agcaaaatat
    12601 cattcagccg ctggtgttaa gtttgctgca tctttgccgt aacgtggtga atatgccgcc
    12661 gggcaattcg cagccgcgcg gcgattttct ctatcacagc atttgtaact gggttcagga
    12721 taattatgcc cagccgctca cccgcgagag cgtggcgcag ttttttaata tcacgcccaa
    12781 tcatctgtca aaactgtttg ctcagcatgg aacgatgcgt tttatcgagt atgtgcgttg
    12841 ggtgcgaatg gcgaaggcga ggatgatttt gcagaaatat catttgtcaa ttcatgaagt
    12901 ggcacagcgt tgcggttttc cggatagcga ctatttttgt cgcgttttcc ggcgtcagtt
    12961 tggtctgacg ccgggagagt acagcgcccg ttttcagggc taacgtcaga aggttaattc
    13021 tgtttccagc agcgtcagga tactttgtgc atcttgcgcg gcaaacaacg actggcggaa
    13081 gtttttattc accagtttac gcgccagctg cgagaagact ttcacatgat tcatcccttc
    13141 gttagcaccc agcgtcagca tgatcaccag ttcgacttcg cccatttctg actgccagtc
    13201 aaccggtttc gccagccggg caatgctgat actggaatga cggatccact gagatttggt
    13261 gtgcgggatc gccacgccaa aaccaacgcc agtggtgaca atctcttccc gctgccagac
    13321 atcctcttcc agctcgaacg gatgttcagt gcgcccgtta acgccgaggt taccgcacag
    13381 gaactggatc gcctgctctt tattgctaaa ggcctgatca acgaagatat tctccagcgc
    13441 cagcagcggg cgaacgtctt cttccggcgt aaaggcggtg agtaacgctt caatttcctg
    13501 cgcgctgcga cattcacacg cctgacgtgc cagttcccga cacgcctcgc tatccagttg
    13561 acgaagctgg cttttcaccg ccggaatgcg cgggctactc atactcagct catccaggcc
    13621 cagcccaagc agtagcggca gataacggct ttcaccgccc agttcaccgc aaatgcctac
    13681 ccatttgccc cgctgatgcg cagtggtaac tatttgctgc aacatgcgca ggaacgatgg
    13741 cgtaatcggg ttatatagcg gcgatacgcg cgggttatta cgatcgaccg catacagata
    13801 ctgggtcata tcgttggagc cgatactgaa gaaatcgacc tcatcgcaga agtggtcgat
    13861 gatgtagcac accgacggca cttccaccat gatacccagc gtaatcgtct ctgcatgacg
    13921 caggccatcg cgcttaagct caacgatcgt tttttggatc tcgcccttca cccataagat
    13981 ctgatcgagg ctgtggacca tcgggatcat caactgggcg ttgccgaaac tggcggcgcg
    14041 caaaatggcc cgcagttgag tgcggaacag gccagcaaat tccgggtaaa tacgtaccgc
    14101 gcgatagccg aggaacgggt tctcttcctg gggaatattc agataaggaa tgcttttatc
    14161 gcctccgatg tccattgtgc ggaagataat cggcttgtcg cccgctgcca gtagcacctg
    14221 ctggtaggct tcaaattgct cctgctcgtc cggcgcgctg tcgcgatcca tatagagcat
    14281 ttcggtacgg aacagcccga cgccttccgc gccgttggca aacgcgcctg gcgcttccag
    14341 agcggtgcca atattcgccg cgatatcaat acgcttgtta tcacgggaat aagtcagctg
    14401 cgcggcagcc tgcgcctgtt gtttttggcg tttatccgcc agcgtttgcg cgacctgata
    14461 ataaccgctc accgcgtcat tcgggttaat cgccagcacg ccgcactggg cgtcaagcac
    14521 tgcaggttgc ccggcataac gggcaatcgc atccagcggc aggccgctca gtaccgggat
    14581 cgccgaggcg cgggccagaa tcagggtatg cgaggtgcgc ccggttttct ccagaatcat
    14641 gcccgcaaga ttttttaaat ccaggcttaa aaactggctt ggggttaaat cctcggccac
    14701 cagaatagtc ggtttttcca gcaccagatt gttgcgcggc ttcagttccg gccaggtaat
    14761 atgcagcaac tgttcgctaa tatcgcgaat gtcgctaaca cgttcgcgca gataatcgct
    14821 ggtagaagca gaaagtttgg cgcaaacctg ctccatattg ctgatgatcg ccgcccccag
    14881 cccctgatgc tgttctgtca tcaggcgacg gatattgcct gcaaattcat catcctgaat
    14941 cagcgacaaa tgggcgctga ggatagtttt gctttcgccg tcacgctcac gcaattgctg
    15001 gttcagttgc tcggcaagcg ttgccaggct gtgctccagc cgggtggaat cttgcgcact
    15061 ggcggggatt gcccgataac tgtcgaggct gtcgctttgt aacagggtca gcgtacccac
    15121 gccgacgccg cttgccagca cattgccgta cagtaaatcc gggttaaggc ggcttaatga
    15181 acgcggcagc ggatgcgccg tcagttctgc ctgcgtaggc tgaacgctgt cgctgtcgat
    15241 aaagcgcacc tggatgtact cttccagcac gcgccgcgcc tgctcttcat cgctgccgct
    15301 aatgttcagg ctgcaactgt cattaaatag ggtgccggtg ccaatcagcg ccagcgagct
    15361 tttggcatcg gcctttgcgt tctggcgatg gttaataaaa gtgatttcgc tttgccactg
    15421 gctgcactgt tctttaagtt cccacgccgg acgagcatgt agaccgttag gcaaaggaca
    15481 gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc tgaaatccgt
    15541 cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa gttgctccat
    15601 ctgcaaaatg tcgcggcaat cggcaatcga cgcggcgcaa tgtccgtggc gggtggcagg
    15661 ccccatcacc actgtgggta cgccagtacc ggttaagtgc accgctccac cgtccgtgcc
    15721 gccgttactg aacatatccg cctgtaacgg cacgccaatt tctgccgcca cggtttcgat
    15781 ccaggtggtg agttttggcg gcgcaatcag tgacttgtcg cttaacacca gcatcgggcc
    15841 gttaccaatc tggcgatggt tagccgcgcc ataatcaaag tttttcgccc agcaggcggt
    15901 atcaaggaca atggcgacgt ccggcgacac cgcgcgggtg gcagtttgcc cgccgcgtaa
    15961 ccccacctct tcgctggaac tggccaccag ccacacttcc gcaggcagtt cagcgctgtg
    16021 tagttcgcgc agtaacgtca ccagcaggta gcaaccgagg cggtcatcaa aggctttccc
    16081 catcacccgc tggtgaggga gaacctgaaa agtagtatca aacgtgacgc gatcgcctgg
    16141 acgaattccc gcctgcatca cttcgtcata cgagcgcgcg ccaatatcca cgcgcatggc
    16201 gctgacatca ttcccctgcc ggtcgccgtc aagcaggcct ggaattttgc actcttcacg
    16261 ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc caaccggcag
    16321 cacatcaatt gcgccttcgc gggagatact gcgcaccata aatccaactt cgtccatatg
    16381 cgcacagagc atcactttcg gacctgtcga ttcattgagg cggatcagca ccgatcccag
    16441 cccatcaaat cgcacttctt tttgcaggcg atccgcttct tccagcagga tctgccgcac
    16501 ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata gcgataaatc
    16561 cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac ttctgcgcct
    16621 tgcggggtga ccagcacaac atcctcgatg cgcacgcccc cttgccctgg caaataaatc
    16681 cccggctcca cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg cggtgaaaaa
    16741 cgcggatctt cgtgtacttc aatgccaata gcatgaccgg tgttgtgacc aaaatagtcg
    16801 ccaaatcctg cctcggtaat cacccgacgc gcggcgtcgt caacctgctg gcagcgcacg
    16861 ccggggcgaa ttgcagagat tgctgcgagc tgtgcctgca aaacaatctg atagacgtta
    16921 aacagcggat gagattcggc gctcacccct tcgccattca ccagcaaggt gcgcgtcata
    16981 tcagagcagt agccctgata gagcgcaccg aaatcgagag tgacaaactc gcccgctgca
    17041 acaatcttgt cgctggcttt gccgtgcggc agcgccccac gccagccact ggcaacaatg
    17101 gtgtcaaaag aggctttttc tgcgccctgc tggcgcataa accactccag ttcagcggct
    17161 atctcgcgct cgctcatccc cgcctgaata aagcggcgaa tatgctctgc accgcgatcg
    17221 gcaatcccac aggcaaggcg gattttctcc acctcctctg gcgttttgat ttgccgcagc
    17281 acatccggcg tggcgctaac cagtttcgca ttgagttcag actgccagcg gtgcgcggtt
    17341 tcccaactca cctgctggcc ttcaaaaccg agcgtttgca actgctcatc ggcaatgatt
    17401 tgcctggcga tggttgtaag cgtgtacgtc gcgtcaagca aatgcagctg gtaaccttgc
    17461 gtgcgggctt ctacatccgc gtaatagcgc gagtccacca gaatgtgcgc actttcacgg
    17521 ctaatcagca catagcctga tccggtggag atccccagat gcggctgttt gttctgccgt
    17581 gaggagagaa gcaccgcatc cagttgttgc gccttaagcc agtcacgcag cgaagcgagt
    17641 aatgtcatct ggattttcct tcttacaggc tatcgattaa caatttgcct ttacggaaca
    17701 tcatcagacg caggaacacc accatcagcg cagttatgac tgcccccagc gcgatccccg
    17761 ccatatagac gcccaggtta gtgaccagcg gccatgccca gatagcggat tccgggaacc
    17821 attgcactgc gcccagccag actgcggcgg ttgagccgac aatcgcgccg accatatacg
    17881 acggaatggc ggtgatgggg ctttccagcg caaacggaat cgcaccctca ctgatcccca
    17941 taaaggcaag gaacatcgcg gtcttaccct gcggataaag ttgtgcgctg aacaggcgtt
    18001 tgccggttaa acgacggtcg ataatggtcg ccagacccag accaatcggc ggaataacga
    18061 tagcgataga acgtgcggtg accggtaata cgtggtcagt ggtaaagctg aaggcaacaa
    18121 aacctgcggc tttgttgatc gggccaccaa ggtcgattgc tgtcgccgcg gcgatcccca
    18181 ttgcgtacat cagcgcgccc ttctcacctg cggcagtcag cacagtacgg ataccgccgt
    18241 tgatccagcc cccaaagggg gtgatgacgt agtacatcgc cagcatgaca aaaatggcgg
    18301 aaagaatcgg tagcaaaaat gtggttttga acgctaacag gaaatccggt aactggattt
    18361 tctggttcat ccatttcaca aggtaaccag caacgataga tataatcagc gcaccaatga
    18421 aggtggacgg caccggcgaa gaggtcgccc attgcatagt gctgggatcg aagttcagca
    18481 gttgcgtcgg ctgggtagac atcaagccgc cgataaaacc agccgggaac gccagtttgc
    18541 caccgataga gttcgccaca aatgcagcaa acatcggaat ggcaaaacca aacaacacgc
    18601 cgccgaagga ctgcgacagc caggcaaatt taagtaagga gaggtcgaaa ccggagaatt
    18661 tcccgctatt aagagcatcc atgatgccga tctcagcagg aattttaagc cagctataag
    18721 caatcaactg gctgaaagcg aggatcacac cgcccataat taaggtcggc accatgcgtg
    18781 aaatccctga catcacatgc tgcgggagtt caccccagaa gctggtttta gaggcctgcg
    18841 gattcttaac tgccgctgcc gcaccggatg cgccaggcac aacggttgca ctgcgttttt
    18901 taatggccat aacaattatc cctgtcgaat attactgttg ttcagaggca atcatctctt
    18961 cgatttcttt gatgatgccc gcagcgtttt taattgcgtc ctgcaaagtg atttcataaa
    19021 cgtcgcgtga ttcgaaacgt tcgttatctt ccggggtaac tgccacggag tggatgatga
    19081 tggtcgcttc ggcgatatcc tgcgccgtca ggcgattctg gataccgtcc gcgccctggg
    19141 tttcaatttt cacttcataa ccggcttcta ccgctgcttc ttccagcgcc tgagcggcca
    19201 taaaggtgtg agccaggccc atcgggcagg cacataaggc aatcagtttc ttactcatcg
    19261 ttaattcctc gttcagtgat agttgacgag tgaaattatg aacgtgcaaa acaaatcgcc
    19321 gttaccagac aaataatgca tttactggag aattaatccc gctgtacgga agtgtgacgc
    19381 aggtcggcct tgtgagggga cacaaagaaa aattgaatta actgtatgat ttaaaagatt
    19441 atcgggagag ttacctcccg atataacagg aaggatttac agaatgtgac ctaaggtctg
    19501 gcgtaaatgt gcaccggaac cgagaagacc cggattgtca tggacgatga gatacaccgg
    19561 aatatcatgg acatattctt taaagcgccc tttatcttca aatgcggcac ggaaaccaga
    19621 ggctttgaag aactcaagga agcgcggcac gataccgccc gcaataaaca cgccgccaaa
    19681 tgtcccgaga ttgagcgcca gattgccgcc aaaacggccc ataatgacgc aaaacagcga
    19741 caacgcacgg cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa tatcttttgg
    19801 cttgagattt tctggcaggc ggttgtcggc tttaacaatt gcgcgataca aattcaccag
    19861 tcctgggcca gaaagcacgc gttccgccga aacatgacca atttccgcac gcaatatttc
    19921 gaggataatg gcctcttctt cactattcgg cgcaaaatca acgtgaccgc cttcgcccgg
    19981 caagcttacc caacgcttat cgacatggac cagatgcgca accccaagcc ccgttccggc
    20041 accgtaaacc gcaataggct taccttctac cggttctgcg ccaccaaact gaatcagatg
    20101 ctcttttttc agcatcggga tcgccatcga tacagcggta aaatcgttaa taatttccag
    20161 atggctaaaa ccgagattct ttttcatttc ggcaattgag aacgcccagg tatggttggt
    20221 cattgccacc cagtctccgg taatcgggca agcgatggca atacagccgt ctttcacctc
    20281 gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg aggctggggt aatcaagccc
    20341 tgaataggtc ttagcctgcg agatttcacc actggcaata tcacacagag caagacgtgc
    20401 gttggtgccg cccacatcac cgactaatgc atactttgtc attcttcaac tgctccgcta
    20461 aagtcaaaat aattctttct cacactgtaa atacctgggg gcataacaac aacgccggaa
    20521 aggcaggctc cctgtaaata tcgatctggg tcacacaatt actttatcgt ttcagcacca
    20581 attgcagcga tgcctttttg caagctgggc aaactaagta tctgaccccg cataaggaat
    20641 agaacatgct ccatccgcga gccagaacca tgttgttatt atcgctcccc gccgtggcaa
    20701 ttgggattgc gtccagtctt attctgattg tggtgatgaa aatcgcctcg gtattacaga
    20761 atttgctctg gcaacgactg ccgggaactc tggggatagc ccaggattca cccctctgga
    20821 tcatcggtgt attaacgcta acgggtattg cggtggggtt ggttatccgt ttcagccagg
    20881 gtcatgccgg accagacccc gcctgtgaac cgctgatcgg cgcaccggtt ccgccctctg
    20941 cgctaccagg acttatcgta gcattaattc tcggtcttgc tggcggcgtc agcctggggc
    21001 cggaacatcc gatcatgacc gtcaatatcg cccttgcggt tgccattggc gctcgtctgt
    21061 taccgcgcgt caacagaatg gagtggacta ttttagcctc tgccggaacc atcggcgcgc
    21121 tgtttggtac acctgttgcg gcggcgttga tattttcgca aaccttaaat ggcagtagtg
    21181 aagttccgct atgggatcgt ctttttgcgc cgttaatggc ggcagcagct ggtgcactta
    21241 ctaccggatt atttttccat ccccattttt cactgcccat tgcgcattac ggacagatgg
    21301 aaatgaccga tattctcagc ggtgcaattg tcgcggcgat tgccatcgca gcagggatgg
    21361 tcgccgtatg gtgcttacca cggttgcacg cgatgatgca tcaaatgaaa aatccggtgc
    21421 tcgtgctggg tattggcgga tttattctcg gtattctggg ggttattggt gggccggttt
    21481 cgctgtttaa aggactggat gagatgcagc agatggtggc aaatcaggct ttcagcacca
    21541 gcgattactt tttgctggcg gtgattaaac ttaccgccct ggtcgttgct gccgccagtg
    21601 gctttcgcgg tggacgaatt ttcccggcag tgttcgtcgg cgtggcatta gggttgatgc
    21661 tgcatgagca cgtccccgcc gttccagcgg caataaccgt ttcctgcgct attctcggca
    21721 tcgtgctggt ggtaacacgc gatggctggt taagcctttt tatggcggca gtcgttgtac
    21781 ccaataccac attgctaccg ctgctctgta tcgtcatgct tccggcatgg ctgttattag
    21841 caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca gcccccccac gataacgttt
    21901 agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg gctttacctc
    21961 cccgtaatat tgagttaaca ggccgctgac ggcttcgatc gtgaaggaga ataacaatgt
    22021 tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca gcaaacgcag
    22081 gcgaaatcac cctactgcca tcaatcaaat tacaaattgg cgatcgcgat cattacggta
    22141 attactggga cggtggtcac tggcgcgacc gtgactactg gcatcgcaat tatgaatggc
    22201 gtaaaaaccg ctggtggcgt catgataatg gctaccaccg tggctgggat aagcgtaaag
    22261 cgtatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc ggaaaaggcc
    22321 gcggacatgg acaccgccat taaacacatt cgccaatgga gcacaatgcc tgatgcgtct
    22381 tatcaggcat acaaaccggc tctttaacgc gtagggcgga taaggcgttt acgccgcatc
    22441 cgccaatggt gccagatgcg acaccagttg aactacaatc ccagcgccgt tcctaccagc
    22501 aaccagatat tcagcgccac gaccagcacc acaatcaccc agcctgtctg ttttacgcgt
    22561 ttgctgttca ccagatctcc catcaacttg ctgtcactgg tgaaaatcag cagtggaacc
    22621 agcgccaggg cgataccaaa acttaacagc acctgactca taaccagaat ccgtgtcgga
    22681 tctaatccca tcagaatgac aataaatgac ggcaacatgg tgactgtacg acgcacccac
    22741 agcgggatat gaaagcgaat gaagccctgc atcaccacct gccccgccag tgtccccacc
    22801 accgttgagg acagtccggc agcaaccagg cttaatccaa agaccgttgc cgcagcatgg
    22861 cttaacaacg gttgcagcgt caggtaagcc tcatcaagat cggcaacacc agtatgacct
    22921 gaaaagtgga acgccgccgc agctgtagcc atcatcgcca gattgacaaa accggcaata
    22981 gtcatggcga tagccacatc ccatttggtg gcggaataac gttgttgacg cgaaccgcca
    23041 tgtaaatgct gagtgagcga agagtgcaaa taaatcacat gcggcataat cgtcgcccct
    23101 aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta aacttgggat caccattcct
    23161 ttacccagct gcgccaggtt aggctgggag aaaatcaact cgacaatgta agccgcggca
    23221 acaaacaaca gtaacccgcc aatcactttc tccaacggtt tttgcccgcg acgttgcagc
    23281 attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc cctgcaacaa cgaaacacca
    23341 agaatgagtt taaaaccgat cgccgcacca ataaattccg ccaggtcggt tgccatcgca
    23401 ataatttctg cctgaaccca atagaaccac actacgggac gcggatagtg atcgcgaatc
    23461 tgctccgcca gatttttacc ggtggcaatc cctagtttgg cagagaggat ctgaatcagc
    23521 atcgccatca ggttggccca aacgacaacc cacagaagct gatagccgaa gctggcaccc
    23581 gcctgaatat tggtcgcaaa gttaccggga tcgatataac caatcgccgc aatgaacgca
    23641 ggtcccatta atgcgagcct catcttgcgc gccgcccgtc cgctgctact ctcaacgcga
    23701 tagttcgtca tcttgtgcct ctaaaacata gcctttgcta tgtttcatgc tatgccaaac
    23761 gagaatgatt atcaaattca tttaaatggg ttgtggtgat ttctctgata gaccaggatt
    23821 atgactacga aaagattgat gacttgaatg ttatggttat gtttaatgtt agcacattta
    23881 cataactttc agcttccata cacaacatag cagaaatgta tgacagctca ctatttttga
    23941 agcttgtcac aggacgtcat tatagtgtgt gtcagatctc gttttcctta accatgttac
    24001 atagaatgtg cacggaaatt taacctgcct catatttgga gcaaatatgg accgcgtcct
    24061 tcattttgta ctggcacttg ccgttgttgc gattctcgca ctgctggtaa gcagcgatcg
    24121 caaaaaaatt cgtatccgtt atgttattca actgcttgtt atcgaagtgt tactggcgtg
    24181 gttcttcctg aactccgacg ttggtttagg cttcgtgaaa ggcttctccg aaatgttcga
    24241 aaaactgctc ggatttgcca acgaagggac taacttcgtc tttggtagca tgaatgatca
    24301 aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc gtctttatct ctgcactgat
    24361 cggtattctc cagcacattc gcgtgttgcc ggtgattatc cgcgcaattg gtttcctgct
    24421 ctccaaagtc aacggcatgg gcaaactgga atcctttaac gccgtcagct ccctgattct
    24481 gggtcagtct gaaaacttta ttgcctataa agatatcctc ggcaaaatct cccgtaatcg
    24541 tatgtacacc atggcagcaa cggcgatgtc caccgtgtcg atgtccatcg ttggtgcata
    24601 catgacgatg ctggagccga aatatgtcgt tgcggcgctg gtactgaaca tgttcagcac
    24661 ctttatcgtg ctgtcgctga tcaaccctta ccgtgttgat gccagtgaag aaaacattca
    24721 gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg ctgggtgaat acattctggc
    24781 aggtttcaag gttgccatta tcgttgccgc gatgctgatc ggctttatcg ccctgatcgc
    24841 cgcgctgaac gcactgtttg ccaccgtgac tggctggttt ggctacagca tctccttcca
    24901 gggtatcctg ggctacatct tctatccgat tgcatgggtg atgggtgttc cttccagtga
    24961 agcactgcaa gtgggcagta tcatggcgac caaactggtt tccaacgagt tcgttgcgat
    25021 gatggatctg cagaaaattg cctctacact ctctccgcgt gctgaaggca tcatctctgt
    25081 gttcctggtt tccttcgcta acttctcttc aatcgggatt atcgcaggtg cagttaaagg
    25141 cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt ctgaagctgg tttacggctc
    25201 taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg gtgctgtaag accatacata
    25261 aaaaagccgg gga
//
LOCUS       DAZUCE010000053        25195 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZUCE010000053 DAZUCE010000000
VERSION     DAZUCE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.053"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(108..1151)
                     /locus_tag="ACHY03_001836"
     CDS             complement(108..1151)
                     /locus_tag="ACHY03_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS3905075.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAVGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLRRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
     gene            complement(1407..2672)
                     /locus_tag="ACHY03_001837"
     CDS             complement(1407..2672)
                     /locus_tag="ACHY03_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3905076.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAERKPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            complement(2870..2945)
                     /locus_tag="ACHY03_001838"
     tRNA            complement(2870..2945)
                     /locus_tag="ACHY03_001838"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2910..2912),aa:Phe,seq:gaa)
     gene            complement(3051..3758)
                     /gene="yqgA"
                     /locus_tag="ACHY03_001839"
     CDS             complement(3051..3758)
                     /gene="yqgA"
                     /locus_tag="ACHY03_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFS3905077.1"
                     /translation="MVIGPFINASAILLGGMLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3911..3994
                     /gene="yqgH"
                     /locus_tag="ACHY03_001840"
     CDS             3911..3994
                     /gene="yqgH"
                     /locus_tag="ACHY03_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFS3905078.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            4156..6291
                     /gene="speC"
                     /locus_tag="ACHY03_001841"
     CDS             4156..6291
                     /gene="speC"
                     /locus_tag="ACHY03_001841"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFS3905079.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQHWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLKLNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(6341..7597)
                     /gene="nupG"
                     /locus_tag="ACHY03_001842"
     CDS             complement(6341..7597)
                     /gene="nupG"
                     /locus_tag="ACHY03_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFS3905080.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7799..8878)
                     /gene="mltC"
                     /locus_tag="ACHY03_001843"
     CDS             complement(7799..8878)
                     /gene="mltC"
                     /locus_tag="ACHY03_001843"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFS3905081.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(8943..9218)
                     /gene="yggX"
                     /locus_tag="ACHY03_001844"
     CDS             complement(8943..9218)
                     /gene="yggX"
                     /locus_tag="ACHY03_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFS3905082.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(9246..10298)
                     /gene="mutY"
                     /locus_tag="ACHY03_001845"
     CDS             complement(9246..10298)
                     /gene="mutY"
                     /locus_tag="ACHY03_001845"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFS3905083.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     IFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            10459..11178
                     /gene="trmB"
                     /locus_tag="ACHY03_001846"
     CDS             10459..11178
                     /gene="trmB"
                     /locus_tag="ACHY03_001846"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFS3905084.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            11178..11504
                     /gene="yggL"
                     /locus_tag="ACHY03_001847"
     CDS             11178..11504
                     /gene="yggL"
                     /locus_tag="ACHY03_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFS3905085.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            11688..12407
                     /gene="yggN"
                     /locus_tag="ACHY03_001848"
     CDS             11688..12407
                     /gene="yggN"
                     /locus_tag="ACHY03_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFS3905086.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            12583..13629
                     /gene="ansB"
                     /locus_tag="ACHY03_001849"
     CDS             12583..13629
                     /gene="ansB"
                     /locus_tag="ACHY03_001849"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFS3905087.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            13746..14753
                     /gene="yggM"
                     /locus_tag="ACHY03_001850"
                     /pseudo
     CDS             13746..14753
                     /gene="yggM"
                     /locus_tag="ACHY03_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
     gene            complement(14996..16132)
                     /gene="hemW"
                     /locus_tag="ACHY03_001851"
     CDS             complement(14996..16132)
                     /gene="hemW"
                     /locus_tag="ACHY03_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFS3905088.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVQTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(16125..16718)
                     /gene="rdgB"
                     /locus_tag="ACHY03_001852"
     CDS             complement(16125..16718)
                     /gene="rdgB"
                     /locus_tag="ACHY03_001852"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFS3905089.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREQKSAISHRGQALKLLLDALRNG"
     gene            complement(16726..17016)
                     /gene="yggU"
                     /locus_tag="ACHY03_001853"
     CDS             complement(16726..17016)
                     /gene="yggU"
                     /locus_tag="ACHY03_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFS3905090.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(17013..17579)
                     /gene="yggT"
                     /locus_tag="ACHY03_001854"
     CDS             complement(17013..17579)
                     /gene="yggT"
                     /locus_tag="ACHY03_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFS3905091.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGHMLLPGLWMAL"
     gene            complement(17597..18301)
                     /gene="yggS"
                     /locus_tag="ACHY03_001855"
     CDS             complement(17597..18301)
                     /gene="yggS"
                     /locus_tag="ACHY03_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFS3905092.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            18319..19299
                     /gene="yggR"
                     /locus_tag="ACHY03_001856"
     CDS             18319..19299
                     /gene="yggR"
                     /locus_tag="ACHY03_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFS3905093.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLKNGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSCCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLNCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(19490..19906)
                     /gene="ruvX"
                     /locus_tag="ACHY03_001857"
     CDS             complement(19490..19906)
                     /gene="ruvX"
                     /locus_tag="ACHY03_001857"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFS3905094.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(19906..20469)
                     /gene="yqgE"
                     /locus_tag="ACHY03_001858"
     CDS             complement(19906..20469)
                     /gene="yqgE"
                     /locus_tag="ACHY03_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFS3905095.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(20578..21528)
                     /gene="gshB"
                     /locus_tag="ACHY03_001859"
     CDS             complement(20578..21528)
                     /gene="gshB"
                     /locus_tag="ACHY03_001859"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFS3905096.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(21541..22272)
                     /gene="rsmE"
                     /locus_tag="ACHY03_001860"
     CDS             complement(21541..22272)
                     /gene="rsmE"
                     /locus_tag="ACHY03_001860"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFS3905097.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(22352..23059)
                     /gene="endA"
                     /locus_tag="ACHY03_001861"
     CDS             complement(22352..23059)
                     /gene="endA"
                     /locus_tag="ACHY03_001861"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFS3905098.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(23154..23651)
                     /gene="yggI"
                     /locus_tag="ACHY03_001862"
     CDS             complement(23154..23651)
                     /gene="yggI"
                     /locus_tag="ACHY03_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFS3905099.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(23728..25122)
                     /gene="galP"
                     /locus_tag="ACHY03_001863"
     CDS             complement(23728..25122)
                     /gene="galP"
                     /locus_tag="ACHY03_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFS3905100.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         6222 a         6323 c         6581 g         6069 t
ORIGIN      
        1 atattcagaa gataacccct ggctgacaac caggggaagg ggacattaac agtgttatta
       61 ttggtggata aattattact tgtgtgcagg atacactaat gcaatatcta ctgatactgt
      121 atgccagtat gtttcatgcc tgccgctgaa aacggcaata tccagggcca gacatattca
      181 ccattcatat ttctgcattt ctgtacaatt ttctcagaaa gcgtctcatc aattctttca
      241 cgcaacttct cctgctgtct taaaatactt tcgcatattt cgaaatcatc ttcctgcatc
      301 agcatttcgg cgcatttaat gatttctgcc cgttcatgca gcacagagga atgattatag
      361 agccagttta caaacagcaa tgcttccgga tattgccccc ataacgggtg gtatgtggca
      421 taataatctt cggcagtatc cggaccgcct gaaaagttat gccatacagc aagtggaaat
      481 ggatgcagat ttccgcacag tatttctgcg atgtaagtat ttgccgcaaa gccgcgacga
      541 aatgcagttg cagcttttac cagttcccca ttaagaaaat gcagaaggcc gagttcatac
      601 cagtaaggtg aaaattcatc tgcatgttcc tgcagtatat ggcgtgcctg ttcatgagcg
      661 ccagtgcgta acagttccgg tcccagtagc cagcgtgcac catgattatc ttcgggattg
      721 taatccagta ttttttcaat gagcatgatg gcatcctgat gacgccgtaa atgtgcattt
      781 gccaggattg ccgcatagag ggcacgaaga aaaggtcggt tgtcaggatg tatccagatg
      841 attctgccac tgaaaccttc agggataaga cgattgccaa ctgccagccc tttaagggca
      901 gcattcagcg ccttacgcgg ggcattctgc tccagaaata cgtatgccaa gtgagcgtgt
      961 acatcaataa agtcattctc ctgtcgggca aggcgcctga gtttgttgat gtaacttgtt
     1021 tcgctgattt catccgcatc gtatgcatca atcagttctt caaactcatc cagcaacgag
     1081 ccaaaccagg tttccggaaa tatgaaacag ccctggttat cgttcacttc aaagcgtaat
     1141 ttgccagtca tattctgaac ctgtaaaaaa ggatggacca taatctgcag gctataaaaa
     1201 ttgtgatgcc tggcatcggt gtccttttat tgtccgggat taacgttgcc catgataata
     1261 cagtgaatcc tgttctgtgg taagacgttg agttattgaa tgagaacctc agcctggctg
     1321 atggttccgc cggcataaaa aagaaattga ttgaggcggg tgctcttccc cttaagagga
     1381 agagcatgtg tgtaaatggt gttcagtcac cttttcagtg gattgttaac ttttgcatat
     1441 tcaaacggac tgatacactc ctccctgttt gcatccagaa aatctgccca ccattgcagc
     1501 ataagccgac gttcttccag atgttctgct ttatgaatat acgcggcacg tactccgttg
     1561 cgctcctgat gactcatctg acgttcaacc gcatctcttg accataagcc ggattcaatc
     1621 aatgcactgc acgccatcgc ccggaatcca tggccacaga cttccttggt tgtgtcatac
     1681 cccatcactc tgagggcctt atttacggta ttttcactga ttggtttacg cgggtcatgt
     1741 gctcccgtaa agatcagacc attttcacca gcccatgtct gaagttctgt cagtatggct
     1801 acagcctgtt tgctgagtgg cacaagatgt ggtgttcgca tcttggagcc ccggtgtgag
     1861 aattttaccc ctggcagagg tttgcgttcg gctggaatag tccacatagc attgtcaata
     1921 tcaatttcag accagcgggc atatctgagt tcactggaac gaataaaaac cagtaaattc
     1981 agcataacgg caagccgggt gacaggctgg cctttataac cgtcaatacg cgacagaagt
     2041 tcaggcaggc gtgaaagatt cagagcgggg cgatgctggc gttttaccgt ggtcaatgca
     2101 ccagccatat ccagagccgg gttatatctg atgatgccag actgtacggc gtaacgcatt
     2161 acagcagata ttctttgctg cagacgactg gcgatttcat aaatttgttt tgcttctgca
     2221 gcacgaacag ggataagcag gtccggggta ttgagtgttg tgatatcccg gttgccgata
     2281 aaggggaaaa cgtgtgtctc gaggcttttg agtactttat cgctgtgtga ttgtgcccat
     2341 tgtttattac tggcatgcca gcgtcgtgca atatgttcaa aagagagctg taaatcaggg
     2401 actacttttt taactctttt ctgttcgcaa gggtcaatcc cctccgaaat cagttttcgg
     2461 gcctcatccc gttttgtacg agcttcggag agagaaactt ctggatacac accaagtgcc
     2521 agagtcttct ccttacccag aatacgataa cggagacgcc agtaacggga accattagga
     2581 tgaaccagca ggaacatgcc agccccgtca gtgagtttat aagccttgtc agtgggcttt
     2641 gcagcccgga tttttgcgtc agtcagtgcc atagttcagc cttctgaatg cgttaaagag
     2701 ggtataaatt ctgtcgaact ggggtgtacc cgcaattata cccacacaat gaggttgatg
     2761 tggggggaat ctggttgagt tcggtagaat tgatttggag gaagaacact taaatcatgg
     2821 tgttctgaaa cgaaaaacgg acctccgtgg aggtccgttg atatgaattt ggtgcccgga
     2881 ctcggaatcg aaccaaggac acggggattt tcaatcccct gctctaccga ctgagctatc
     2941 cgggcaacgg ggcgcattaa acctgattcg cctcgtctcg tcaatcaaat ttcttcaatt
     3001 tactgcagac tgcacaatct atcatcactt tgccgctatt gcacgcattg tcaggcaaac
     3061 caggcggtcc aggcggcgga aagcggcatt gccagaagga gtgctggaag catgttgacc
     3121 accgggaaca ttttaatgcc acagatgcgt aatccggttg ccagcagcaa taaaccgcct
     3181 acggcgctga agtctgccat catcgacggt gtggtcagcg gtaatatcag cgcggcagcc
     3241 cacgccagcg ttaactggat gatcagtaat gggatactaa tcaccgatac cgcaataccc
     3301 agcgagcagg cgaagatcat cgccgtaaag aaatcaagaa atgacttggc gattaaaata
     3361 ctcggatcgc cggtcatccc ttcgttcatc gccccgaaga tcccggtgcc gctggcgcaa
     3421 aacaggacaa taatcgcgac ataattctga ataaaagatt catgcgctgg cttcttacgt
     3481 gagtgacgaa acagattttg tgctttggcg accgctgtat tgacgccttt ctccagcaga
     3541 caaatttcgc cgattaatgc tccgagtaag gttgctaaaa ccatcgctgg aaggttggcg
     3601 catttcacca ccagtaaaat accaatcccc agcgatgcca gaccaaaaat tgatgtcatg
     3661 gagacgcgga tacgttccgg taagcgttgg ctaagcagtg caccgagcat gccacccagt
     3721 aatattgcac ttgcgttgat aaaagggccg atgaccacaa gagttcctgt taactgtatt
     3781 gtttgatttt cttattatga actttttgcc ttgttgatgg cttgtctggt cgcaaagtcc
     3841 tggcttgcac gccttagcga aaggtgccat gattgcgcga attttctcct ctctgtacgg
     3901 agtttgcccg atgcacgcca tctccttaca ttctctcgct tatcgccgtt tcgcgcgaaa
     3961 cgtttccctt tctatgttac tgctcatgcg gtgaagttaa cgcacgctca ctgcaggaca
     4021 acagtaaaat cagagcgttt ctgcttttac tgatgtctgg cggtcggagc tggtgaccag
     4081 tttgacccat atctcatggg gcagggtttt ccaccttgtc cggtattctt acttccccga
     4141 aacgggtttg cgcttatgaa atcaatgaat attgccgcca gtagtgaact ggtatcccga
     4201 ctttcttctc atcgtcgcgt ggtggcgttg ggagatactg attttacgga cgtcgcggca
     4261 gtcgtcatta ccgctgcgga tagtcgcagt ggcattctta cgttgcttaa gcgcaccggt
     4321 tttcatctac cggtgttttt gtattccgaa catgctgttg aattacctgc gggcgttacg
     4381 gcggtaatca acggcaacga gcagcattgg ctggagctgg aatccgcagc ctgtcagtat
     4441 gaagagaatt tgctgccacc gttttatgac acgctgacgc agtacgttga gatgggcaac
     4501 agcacctttg cttgccctgg gcatcaacat ggtgcgtttt ttaaaaagca tcctgccgga
     4561 cgccattttt acgatttctt tggtgagaac gtctttcgcg ccgatatgtg taatgctgac
     4621 gtaaaattgg gcgatctgct tattcatgaa ggatcggcga aagatgcgca gaaattcgca
     4681 gccaaagtct ttcatgccga taaaacctat tttgtactga acggcacatc ggcagcgaat
     4741 aaagtggtga cgaatgcgct attaacgcgt ggcgatctgg tgctcttcga ccgtaacaac
     4801 cataagtcga atcatcacgg cgcgctgatt caggcggggg cgacgccggt ctatctggaa
     4861 gcttcacgta acccgtttgg tttcattggc ggtattgatg cgcactgttt taatgaagag
     4921 tatctgcgcc agcaaattcg cgacgttgcg ccagaaaaag ccgacctgcc gcgcccgttt
     4981 cgcctggcga ttattcagct gggaacctat gacggcactg tctataacgc ccgtcaggtg
     5041 atcgataccg ttgggcatct gtgtgattac attctgtttg attccgcgtg ggtcggttac
     5101 gaacagttta tcccgatgat ggcggatagc tcgccgctgc tgttaaaact taacgaaaac
     5161 gatccgggga tctttgtgac tcagtcggtg cacaaacagc aggcgggatt ctcacagacg
     5221 tcgcagatcc ataaaaaaga taaccatatc cgcggacagg cgcgtttttg cccgcataag
     5281 cggttgaata acgcctttat gctccatgct tctaccagcc ctttctatcc gctgtttgct
     5341 gcactggatg ttaacgccaa aattcatgaa ggggagagtg ggcgtcggct gtgggctgag
     5401 tgcgttgagt tgggtattga gtcgcgcaag gctattcttg cgcgctgtaa gctgttccgc
     5461 ccgtttatcc cgcccgttgt tgatggcaaa ttgtggcagg attatccgac atcagtgtta
     5521 gccagcgacc gccgtttttt cagttttgag ccgggggcga agtggcacgg ctttgaagga
     5581 tatgccgcgg atcagtattt tgttgatccg tgcaagctgt tactcactac accaggtatc
     5641 gatgccgaaa ccggcgaata tagcgacttt ggcgttccgg cgacgattct ggcgcactat
     5701 ctgcgtgaga acggcattgt gccggagaag tgcgatctca attccattct gttcttatta
     5761 actccggcgg aaagccacga gaagctggca caactggtgg cgatgctggc gcaatttgaa
     5821 cagcatattg aggatgactc gccgctggct gaggtgttgc cgagcgttta taacaaatat
     5881 ccggtgcgct atcgcgacta caccctgcgc cagttgtgtc aggagatgca cgatctgtat
     5941 gtcagtttcg acgtcaaaga cctacaaaaa gcgatgttcc gccagcagag tttcccgtca
     6001 gtggtgatga acccccagga tgcgcatagc gcttatattc gcggtgacgt ggagttggtg
     6061 cggattcgtg atgccgaagg gcgaattgcg gcagaagggg cgttgcctta tccacctggc
     6121 gtgctttgcg tggtacccgg ggaagtctgg ggtggggcgg ttcaacgtta tttccttgcg
     6181 ctagaagaag gggtgaattt gttgccggga ttttcgccgg agctgcaagg tgtttatagc
     6241 gaaaccgatg cggatggcat gaaacggttg tacggttatg tgttgaagta agaataaaaa
     6301 aaacgggtcg ccttctggcg acccgttttt ctttgcgtaa ttagtggcta accgtctgtg
     6361 tacctgtcgg gacacgaacg tgtttatatt tgaacatcgc catgaacgcg aaggccagaa
     6421 ccacggagta accagcgaaa atcaaccata cggtctgcca gtcggtaatg ccgttttggg
     6481 tgtacatctc aaccacttta ccgctcacga tgccaccgag gatacagccg aagccgttgg
     6541 tcatcatcag gaacatccct tgcgcactgg cgcgaatcgc cgggctaact tctttttcga
     6601 caaacaccga accagagatg ttgaagaagt cgaacgcaca gccgtaaaca atcatcgaca
     6661 gaaccagcag tacggtgccg aacggagtcg ggtcgccgta agcaaacagc gcaaaacgca
     6721 ggatccacgc cacaatactg atcatcatta cgttcttaat accgtagcgg cttaagaaga
     6781 acgggatggt cagaatgaac agggtttcag agatctgcga aatcgacatg atgattgacg
     6841 catgctgcac aataaagctg ctggcaaaca tcggatcttt gtcgaagctg tgcaggaagg
     6901 tattaccgaa catgttggta atctgcagtt ccgcgcccag cagcattgag aagatgaaga
     6961 agattgccat acgcttgttt ttaaacagcg cgaatgcatc gaggcccagc agggttgtcc
     7021 agctctgatt cgcttgctgt ttagcaaccg gaatatgcgg cagagtcagg gtaaacagaa
     7081 ccagaatggc ggaaagtgct gcgccaatat acagctgcat gtggcttaat tcgaagccag
     7141 acaggctcac cacccacatt gccatgataa agccgatggt gccccagata cggattggcg
     7201 ggaagtcagt aacgatatcc atcccggcat tttgcaggcg atagtaagag atggtgttga
     7261 ttaacccaag cgttggcata taagcaaacg agttaatcaa tatcacaagg aacatcgctt
     7321 ccggcgtcgt gacctgtgcc gccatgaaca gcgtgatagc gccaatggtg tggcaaatgg
     7381 catataccca tttcgcactt aaccatttgt cggccacaat ccccagcagc gcaggcataa
     7441 agaccgcggc gatacccagt gaactataaa ctgcgccaat agaagcaccg tcaaacttca
     7501 gggtaacaaa catataggag ccgagggtcg tcagccaact tccccacaga cagaactgca
     7561 gaaaagagag gattttcagc tgcagcttaa gattcatgtt aatttcctca catcgtgatg
     7621 cggatgagtg tttgaaaaag cacatttgac cgtgcgagat gatgggattc tatcaacttc
     7681 gcggactggt tttttgttac ctgtggcaaa taattgcaaa cattcctaat ttgcaaaacg
     7741 aaattgtgat atggataaca tttttgcccc tgagcatcgt caggggcggt tagtggaatt
     7801 atcggcggcg gtaggatttt tgcgcggtat tcactttata aagataacgg cgagattccg
     7861 cagagggatg gcgggtcgtc agcgtttgat aaacatcgcc cggcgtcatg gtgttaataa
     7921 tattagccgc ctgaatctta tcattcgaaa agactcgcag cacgctgcct gcgccgccgt
     7981 tataggcggt gatgacggca taacgccggg atgtagggtt atcaattccg ccgagataaa
     8041 cattgttcag catcgccaga tacgcggtgc cggtatcaat attgctggca ggatcaaaca
     8101 agaaactgcg gctcggcgtg ccggatttcc cctgcgagcg gaacacatct ttcccggcag
     8161 tatgttgtac cacctgcatt aaccccagcg catcggaacg gctgaccgca tacgggttaa
     8221 aggaagattc agtctgcata attgccagaa tcagcgactc atcaacgcca tatttccgtg
     8281 acgcctggcg gaccatgccg agatatttgt gcgcacgttt atcaaggtgg ttcggcacca
     8341 tgttaatggt gacgctgtag atgatacgca gcccgttgct gcggctcttc agacggtttt
     8401 tcagcagata atccgcgaag ttgcttgcgc gaccttccca gcgaatcggc tgcccggtgt
     8461 tgtccaccac ctgaccgtaa aggaaaggtt ctttcgaaat tgtaatatca tcaacgtcgg
     8521 aatagagatc gaccgaactc ggatcgtcac ccatcagcaa cgttttgata attgcccggc
     8581 gcaaatgcgc ggcaggttct gtcccggcga tggtttcgat agtaatcgta ccgtcatcga
     8641 agttgatgtg gctgcgggtc tgatattgat cggtgtattt cacgtagtcc ttaggaccgg
     8701 cgatcaccac ctctttgaat ccccagatgt tctcaatatt gtgggcaaat tgccccatca
     8761 gaatatcaaa accgttggtg tctttgaccc aggcttcgtt ataggtatcg ccttttttgg
     8821 tcgtcgaaca ggagatgagc aacggcgcaa tcaaagccag cgcgagatat tttttcatca
     8881 ttccgggtgc gtgttgtgtt tgtttttatg caagctgccg gaggcgtgct ccggcactgt
     8941 ttttattttt tatcttccgg cgtatagccc tcgatatgca cctctttacc ctcgaacagg
     9001 aagttgacca tctcctgctc cagcagcttg cggtgctcgg cattcatcat gttgagtttc
     9061 ttttcattaa tcagcatggt ttgtttgtgc tgccactgcg cccaggcttc tttggagatc
     9121 tcgttataga tgcgttttcc cagttcgccg gggtacagct gaaaatcctg accttctgct
     9181 tcacgttgca ggaaagtaca aaaaatcgtt ctgctcataa atcatcctct ttatcgactc
     9241 acgcgctaaa ccggcgcgcc agtgcgtaac tgctgtaaca aacgctccac gggagccgcc
     9301 aggccaactg acggcggttg cgctaagtta taccagagcg cattgccttc atccatgcag
     9361 ccggtgaatg acgacacggg aagccacata ggcacaatat ctaagtggaa atggctgaag
     9421 gtatgccgaa acgcggtcag ctgcgtcagg ttatcggcag caatctgccg ttgcgccagc
     9481 cactgccgca aactttcttc gtcggcaaac tgcgggaaac agtataaacc gccccacaat
     9541 ccgctcggcg gacgctgcgc cagcaatact tcatcttcgt gctgtaacag gagaaagtag
     9601 ccggtgcgct ccggcagcgt ctgtttcggt tttttgcccg gataaagcga ccagctattg
     9661 ttggtggcgg caatacatcc gttttgtagc ggacagagcg aacatttcgg cttcgaacgc
     9721 gtgcaaatca tcgcgcccaa atccatcatc gcctgattaa accgttccac gccgaccgcg
     9781 ggcgtcacct gctcgcttaa actccataat ttattctcga cctctttttt cccaggccag
     9841 ccgcttacag catagcagcg cgccagcacc cgtttgacgt taccgtcgag aatcggaaag
     9901 tgcttaccca gagaaagcga gagaatcgcg cctgcggtgg aacgcccgac gcccggcagt
     9961 gctgcaactt cctcaaaggt ttccgggaat ataccgccgt gtaaggtcgc cacttgttgt
    10021 gccgctttat gcagattgcg cgcgcgggcg taatagccaa gcccggtcca caagtggaga
    10081 acttcgtcga gcggcgcatt ggcgagatcg gtcaccgtcg ggaagcgcgc cataaagcgt
    10141 tcaaaatagg ggataacggt cgcaacctga gtttgttgca acatcacttc tgagagccat
    10201 actttgtagg gcgtcttgtc aatttgccag ggcagagttt ttcgcccgta tttatcgtac
    10261 cagtccagaa cctgggctga aaattgcgac gcttgcatgg tcaccgaatt cactgttgtt
    10321 gggggcaaga ttgcagcaca gcgacggcag ggtgtaaacc ggaactttcc gcagccacgg
    10381 ccttatcatg cttgcatccg gcaactaact ttggataatg cccgttttca gaacactttc
    10441 acaagcgact aaatctttat gaaaaacgac gtcatttcac cggaatttga tgaaaacggc
    10501 cgcccactgc gccgtatccg tagttttgtg cgccgccagg ggcgactgac caaaggccag
    10561 gaacatgcgc tggaaaacta ctggccggtg atgggcgttg agttcagcga agatatgctg
    10621 gatttccccg cgctttttgg ccgtgaagcg ccggtgacgc ttgagattgg ttttggcatg
    10681 ggggcgtcgc tggtggcaat ggctaaagat cgccccgagc aggacttcct cggcattgaa
    10741 gtgcattcac cgggcgttgg tgcgtgcctg gcttctgcgc atgaagaggg cttaagcaac
    10801 ctgcgcgtga tgtgtcacga tgcggttgaa gtgctgcata aaatgattcc tgacaattca
    10861 ttgcgcatgg tgcagctctt tttccctgac ccgtggcaca aagcgcgcca taataaacgc
    10921 cgtatcgttc aggtgccgtt tgccgaactg gtaaaaagca aactgcagct ggggggcgta
    10981 ttccatatgg cgaccgactg ggaaccttat gcggaacata tgcttgaagt gatgtcttct
    11041 attgacggtt ataaaaacct gtcagagagc aatgattacg taccgcgtcc ggcatcacgt
    11101 ccggtgacga aatttgaaca acgtggtcat cgtcttggtc acggagtatg ggacttaatg
    11161 ttcgagaggg tgaaataatg gcaaagaacc gtagccgtcg tctgcgtaaa aaaatgcaca
    11221 tcgacgaatt ccaggaatta ggattttcgg tggcatggcg attcccggaa ggtacatcgg
    11281 aagaacagat tgataaaacc gttgatgatt ttattaacga ggttatcgaa ccgaacaaac
    11341 tggcctttga cggcagcggt tatctggcct gggaaggtct gatctgcatg caggaaatcg
    11401 gcaaatgcac tgaagaacat caggcgattg tgcgtaagtg gctggaagag cgcaaactgg
    11461 atgaggtacg caccagcgaa cttttcgacg tttggtggga ctaagaaagc atacgggcga
    11521 tgacaaatgc aaaactgcct gatgcgctac gcttatcatg cctacaaaga tgcacgatcg
    11581 agtaggcggg ataaggcgtt tacgccgcat ccggcatgga aaacgcgtac tttgttatca
    11641 atctggggcc agcaaatgct ggcctgattt gttcttgagg gaagactatg atgcgcaaaa
    11701 tgctgctggc ggcagcactt tcagtgacgg caatgaccgc tcacgccgac taccagtgca
    11761 gcgtcacgcc gcgtgacgat gtgattgtca gcccgcaaac cgtgcaggtg aagggcgaaa
    11821 acggcaatct ggtgatcacg ccagacggca acgtgatgta taacggtaag caatattccc
    11881 tgaatgccgc ccagcgcgag caggcgaagg attatcaggc tgaactacgt agcacgctgc
    11941 cgtggattga tgaaggcgcg aaaagccgcg tcgagaaagc ccgtattgct ctggataaaa
    12001 ttatcgttca ggagatgggc gaaagcagca aaatgcgcag ccgtctgacc aaacttgatg
    12061 cgcagctgaa agagcagatg aaccgcatta tcgaaacgcg cagcgatggc ctgacgttcc
    12121 actataaagc cattgatcag gttcgcgctg aaggccagca attagtgaat caggcaatgg
    12181 gcggaatttt acaggacagc attaatgaaa tgggcgcgaa agcggtgctg aaaagcggcg
    12241 gtaacccatt acagaatgtg ctgggaagcc tgggggggct gcaatcctca atccaaaccg
    12301 agtggaaaaa gcaggaaaaa gatttccagc agtttggcaa agatgtttgt agccgcgttg
    12361 tgactctgga agatagccgc aaagccctgg tcgggaattt aaaataatcc tctattttaa
    12421 gacggcataa tactttttta tgccgtttaa ttcttcgttt tgttacctgc ctctaacttt
    12481 gtagatctcc aaaatatatt cacgttgtaa attgtttaac gtcaaatttc ccatacagag
    12541 ctaagggata atgcgtagcg ttcacgtaac tggaggaatg aaatggagtt tttcaaaaag
    12601 acggcacttg ccgcactggt tatgggtttt agtggtgcag cattggcatt acccaatatc
    12661 accattttag caaccggcgg gaccattgcc ggtggtggtg actccgcaac caaatctaac
    12721 tacacagcgg gtaaagttgg cgtagaaaat ctggttaatg cggtgccgca actgaaggac
    12781 attgcgaacg ttaaaggcga gcaggtagtg aatatcggct cccaggacat gaacgatgat
    12841 gtctggctga cactggcgaa aaaaattaac accgactgcg ataaaaccga cggcttcgtc
    12901 attactcacg gtaccgacac gatggaagaa accgcttact tcctcgacct gacggtgaaa
    12961 tgcgacaaac cggtggtgat ggtcggcgca atgcgtccgt ccacgtctat gagcgcagac
    13021 ggtccattca acctgtataa cgcggtagtg actgcagctg ataaagcctc cgctaatcgt
    13081 ggcgtgctgg tggtgatgaa cgacaccgta ctggacggtc gcgatgtcac caaaaccaac
    13141 accaccgacg tagcgacctt caagtctgtt aactacggtc ctctgggtta cattcacaac
    13201 ggtaagattg actaccaacg taccccggca cgtaagcaca ccagcgacac gccgttcgat
    13261 gtctctaagc tgaatgaact gccgaaagtc ggcattgttt ataactacgc taacgcatcc
    13321 gatcttccgg ctaaagcact ggtagatgcg ggctatgatg gcatcgttag cgctggtgtg
    13381 ggtaacggca acctgtataa aaccgtattt gacactctgg cgaccgccgc gaaaaacggc
    13441 actgcagtag tgcgttcttc ccgcgtaccg acgggcgcta ccactcagga tgccgaagtg
    13501 gatgatgcga aatacggctt cgtcgcctct ggcacgctga acccgcaaaa agcgcgcgtt
    13561 ctgctgcaac tggctctgac gcaaaccaaa gatccgcagc agatccagca gatcttcaat
    13621 cagtactaat cacctcgccc cggtatcgtg ccggggcttt ttcacttcag acttacgtcc
    13681 attgccaatt tttattacca tgatgataat cactggaata aattattccg cgcgagggtt
    13741 ttcgggtgaa aaagcaatgg attgttggca cggcgctgct tatgttgatg actggtaatg
    13801 tctgggcaga tggcgaaccg ccaactgaaa atatcttaaa agatcaattc aaaaagcagt
    13861 atcacggcat tctcaagctt gatgctatca ccttaaaaaa tcttgatgct aagggtaatc
    13921 aggccacctg gtcagcggaa ggcgatgtct cttccaatga cgatctctat acctaggtcg
    13981 gtcagttggc agattacgaa ctgctcgaac agacctggac gaaagataaa gcggtaaaat
    14041 tctcggcgat gttaaccagt aaaggaacgc ccgcgtctgg ctggtcggtg aacttttact
    14101 cttttcaggc ggcagccagc gatcgtgggc gggtggtcga cgatatcaaa acgaataata
    14161 aatatctgat cgtgaatagc gaagatttca attatcgctt tagtcagctt gaatctgcgt
    14221 tgaataccca gaagaactcg attcctgcgc tggaaaagga agtgaaagcg cttgataagc
    14281 aaatggtggc ggcgcagaaa gcagcggatg catattgggg aaaagatgca aacggtaagc
    14341 aaatgacccg cgaagacgcc tttaaaaaaa ttcaccaaca gcgtgatgag tttaataaac
    14401 agaacgatag cgaggcgttc gcggttaaat atgacaaaga ggtttatcaa ccggcgattg
    14461 cggcatgtca taaacagagt gaagagtgtt atgaagtgcc gattcagcag aaacgagatt
    14521 tcgatatcaa cgaacaacgg cgacagactt ttctgcaatc acaaaaactt agccgtaaat
    14581 tgcaggatga ctggataacg ctcgaaaaag ggcaatatcc gctaacgatg aaagtttcag
    14641 aaataaacag taaaaaagtc gcgattttga tgaaaattga cgatatcaac caggctaatg
    14701 aacgctggaa aaaagatacc gaacaactgc ggcgcaatgg ggtgattaag taagttttga
    14761 tatagcgcac gatttgtagc aggatgcggc gttgaccccg catccggcaa tcaacgtctg
    14821 atgcgacgct agcgcgtctt atcagaccta caacagctgc cacatgtcgg ctgaggtgct
    14881 cacgccgcat ccggcaatca acgtctgatg cgacgcttgc gcgtcttatc agacctacaa
    14941 ccgttgccac atgtaggctg gataaggcgt tcacgccgca tccggcaata caagtttact
    15001 cagccagaaa aagctccagc agcgaattta aaaacagctt cccatgttcc gtaatctgcc
    15061 agtaatccgc acattcggtg agataaccct gggcaatcgc ctcgtctaac tgtgggcgaa
    15121 tcacatcttc gcaaagcccg gtatacgcac taaactccac gcgcggcgca ggttccagca
    15181 accggaagcg attcataaag aactcaaacg gcttatctgc ggcttcgaca tcacgctggc
    15241 tttccagata ccttccctgc ataaaaccac gcggatgacg cgttttggtg gtacgcagaa
    15301 tgcgcccatc cgggaaggtc actttgccgt gcgcgccgca gccaataccg atgtagtcac
    15361 caaagcgcca gtagttgaga ttgtgctggc actggtaacc gggtttggcg taagcggaag
    15421 tttcatattg ctgataaccc gctgcggtta ataactgatg cccctgttcg aatatatccc
    15481 acaacgcgtc atcgtccggc agtacaggag ggcgcgagcc aaacagcgta ttgggttcga
    15541 tggtcagttg ataccaggaa agatgcggcg gattcagttc aatggcctgg cgcagatcgc
    15601 caagcgcctc ttccagtgat tgatccggca gcccatgcat caaatcaagg ttaaaactac
    15661 gcagccccag cccgcttgcc agattcgcag ctcgtttcgc ttcttgcggg ccatgaatgc
    15721 gcccaagtcg tttcagcttt tcttcgctaa aactctgtac gccaatagag atgcggttca
    15781 cgccagcacg ctgataatcg acaaagcgat cggcttctac cgtaccaggg ttcgcttcca
    15841 tagtaatttc tgcatccgct gccagcggca aacgcgcacg cacgccgtcc agcagcgttt
    15901 gcatcgccgg gccggaaagc aggctcggcg taccaccgcc aataaaaatt gtctgcactt
    15961 cacggccctg agcgtaagcc acatcgttgt ccagatcgtt aagcagatgc tgaacataat
    16021 cgtcgtgcgg cacttctcct ttcaacgcgt gagagttgaa atcgcagtac gggcatttct
    16081 gcacgcacca cgggatgtga atgtagagac tcagcggcgg taatttaacc attacgtaaa
    16141 gcgtccagca gcagtttcaa cgcctgacca cggtgggaaa tggcgctctt ttgttcgcga
    16201 gtcagttcgg cagcggtttt cccttcggaa ggtacgaaga agattggatc ataaccaaag
    16261 ccaccagtgc ccgccggttc acgagtaatc actcctggcc agctaccgtg gcacaccagc
    16321 ggagtcggat cttccgcgtg acgcagatac accagcacgc agtggaaacg cgcctgacgt
    16381 tggtcgtccg gtacgtcttt cagtgtttcc agcagttttt gcagattctt ttgatcggtc
    16441 gcgtcttcac cggaataacg cgcggagtaa atgcctggcg cgccaccaag tacatctacc
    16501 gccagaccgg agtcgtcggc aattgcaggt aaaccggtca cttttgccgc atggcgcgct
    16561 ttcagaatcg cgttttcgat aaaggtcagg ccggtttctt cagcggaatc tacgccgagg
    16621 tctgtttggg ccacgatatc aagaccgaag tcgctaagca gcgacgccag ctcacgcact
    16681 ttaccggcat tgccggttgc gaggacaact ttttgcatag gatacctaat taattaacgc
    16741 cgcgatttct ggcgggattt gttgcggatt aatgatttta atttgtttgt ggcggccaag
    16801 ttcgcctttt tcaatcacca cctggctttt ggcaacccgg aattgcttac cgagaaactt
    16861 caccagatga ctgttggcct ggccgtcaac cggcggcgcg gttatggcga cttttacctc
    16921 gtcgccatgt aaaccgacaa tagaatcacg gctggctttc ggctgaatat aaagccgtaa
    16981 aaccagaccg tcatcgttaa ctgttacggc actcataacg ccatccacag ccccggcagc
    17041 agcatgtgtc cggttgcctg taatacttct gcgacaccca tattgatgac atacagcagc
    17101 agaacgagga tcatcggcga gaaatcaatc ccacccattg ccggtagcag gcggcgaatc
    17161 gggcgcagca gcggatcggc cagctgaatc agcacgtatt caatcgggct acggccctgg
    17221 cttacccagc tcataatcgc catcaccagc aggacccaga aaatcagcag gccgatggtt
    17281 ttcagcagaa tcagtaaacc ggcaatccag atgattggca ggaaggtcac cactttaaac
    17341 agcacgatgg ctttgataaa actgagaata taggcaacca gcagcgaggc gctatcaatg
    17401 gggcccattg ccggaataac gcggcgcagt ggcccgataa ttggctgcgt taccttcact
    17461 acaaactgtg agaaggggtt gtaaaaatca caatgagccc actgcatcca gatgcgtaat
    17521 aacagcacca tggtatacag ctcaatgacc gttgaaagca ggaaagtcaa cgtattcatg
    17581 gcgttccttt aattccttat tttttagagt aatcacgcgc accaaaaatt gcagtgccga
    17641 tacgaaccat cgtgctacct gccgcaatgg cggcttccat atcgtccgac attcccagag
    17701 agagcgtgtc gatatgcggg tagcgcgttt tcagtccggc aaatgctaca gccatttgac
    17761 gtgcaacttc aaactgcctt acatattctg actcaggcgc agggattgcc atcagcccgc
    17821 gcagacgtaa acgcggtagt tcagcgaccg cagctgccag ctcgtccagt tctgccagtt
    17881 gaatcccgga cttactgttt tcatcactaa tattaatttg aatcagtacg ttaagagggg
    17941 gaagttctgc cgggcgctga tcgttgaggc gggtagcaat gcgcaaacgg tcgatggtat
    18001 gacaccagtc gaaatgctct gccaccaggc ggcttttatt agactgcaac gggccaataa
    18061 aatgccattc taatcctgtt acgcccagtt cctgaaagtg gcgaatttta tctacccctt
    18121 cctgaacgta gttttcacca aattgacgct gcccggcatc aatggcttct gcgatggcgc
    18181 tcgcaggttt tgttttactg actgcaagca gcgtaatttc ttctggagaa cggccgcaac
    18241 gcgttgcagc cgctgagatt ttgtcccgga cctgtgccag gttatgcgca atatcgttca
    18301 ttttccgagg atcttagtat gaatatggaa gaaattgtgg cccttagtgt aaagcataac
    18361 gtctcggatc tacacctgtg cagcgcctgg cccgcacgat ggcgcattcg cggcagaatg
    18421 gaagctgcgc cgtttgatgc gccggacgtc gaagaactac tgcgggagtg gctggatgac
    18481 gatcagcggg caatattgct gaagaatggt cagctggatt ttgccgtgtc gctggcggaa
    18541 aaccagcgat tgcgcggcag tgcgttcgca caacggcaag gtatttctct ggcgttacgg
    18601 ttgttacctt cgtgctgccc gcagctcgaa cagcttggcg caccaccggt attgccggaa
    18661 ttactcaaga gcgagaatgg cctgattctg gtgacggggg cgacggggag tggtaaatcc
    18721 accacgctgg cggcaatggt tggctatctc aatcaacatg ccgatgcgca tattctgacg
    18781 ctggaagatc ctgttgaata tctctatgcc agccagcgat gtttgatcca gcagcgggaa
    18841 atcggtttga attgtatgac gttcgcatcg ggattgcggg ccgcattgcg ggaagatcct
    18901 gatgtgattt tgctcggaga gctgcgtgac agcgagacaa tccgtctggc gctgacggcg
    18961 gcagaaaccg ggcatttggt gctggcaaca ttacatacgc gtggtgcggc gcaggcagtt
    19021 gagcggctgg tggattcatt tccggcgcag gaaaaagatc ccgtacgtaa tcaactggca
    19081 ggtagtttac gggcagtgtt gtcacaaaag ctggaagtgg ataaacagga aggacgcgtg
    19141 gcgctgtttg aattgctgat taacacaccc gcggtgggga atttgattcg cgaagggaaa
    19201 acccaccagt taccgcatgt tattcaaacc gggcagcagg tggggatgat aacgtttcag
    19261 cagagttatc agcagcgggt gggggaaggg cgtttgtgac gttgccatgc ccgatgtgca
    19321 agcttatcgg gcctacaaag gcaataaacg tctgatgcga cgcttgcgcg tcttatcaga
    19381 cctacaaccg ctgccacatg taggccggat aaggcgttca cgccgcatcc ggcaataagg
    19441 cgtaaggcgc gatgatatta tttacactcc agcaggcgtt taaatcgcct taatatccct
    19501 gctcgaaata gctttcgaga ataataaccg cagaggcaga gtcgactttg cctttgttga
    19561 gcgcccgata gccgccctgt tcaaacagac cggaacgggc ttccacggtg ctaagacgtt
    19621 cgtcatggag ctttacttca acaccgaaac gaccatgaat acggttggca aatttacgcg
    19681 ctctggcagt caatggttgc tcggtaccgt ccatattcag cggcaaaccg acgatgattt
    19741 cgtccggctg ccactctttc agtaaacgtt cgatgaggtt ccagtccggc gtaccgtcct
    19801 gtgctttaat tgcaggcaaa gggcgagcgg tgccggtaat gcgttggccg accgctacgc
    19861 caatgctttt ggtgccgaag tcgaaggcaa gtaaggttcc actcatcagg cgtgccctgc
    19921 cacaccaggc atggtgagaa tatccacacc aatcagtttt gccgcctcgc gccagcggtc
    19981 ggcaattggc gttttgaaca gaatattcag atctgccggg gcggttagcc acgcgttatc
    20041 gagaatttct tgttccagtt gacctttttc ccaggaggca tagcccagag ccaccaatac
    20101 gtcagacggt tgtttatcgg tgccgagcgt ttccagcaca tcgcgggaag tggtcatcac
    20161 cgtgttgtct gaaatgcgaa tgctggaagc aaaattggag ggcggagtat gcaaaataaa
    20221 cccgcgatct tcagccagtg ggccgccgag cataaccggt ttatccagac ggattgattc
    20281 atcacgcggc tccggcgtga tcttcagctt ttccagaatc ccttcaattt tgagattttc
    20341 cagcggcttg ttgacgatga tccccattgc accattggta ttatgttcgc aaatgtagac
    20401 cacggaacga cggaaaatcg gatcctggag agcaggcatg gcaataagaa agtgatgctg
    20461 taaattcatt gtcagaggtt ctgtttcctg gttcaaaaaa gcaacagcgc ccagtatgcg
    20521 tggaaagaag ggcgctgtca ctcagagtct caacgagatc cttctcgcta aggtgggtta
    20581 ctgctgctgt aaacgtgctt cgatggcatc cattaacatt ccggtgatcg acaccggaaa
    20641 ctctgcttca atctcacgaa tacaggttgg gctggtgacg ttaatttcag tcagacggtc
    20701 gccgatgata tccagaccaa caaaaatcag ccctttttct ttcagcgtcg gcccgatctg
    20761 acgggcgatt ttccagtcac tttccgtcag cggacgaggt tcaccgcgac caccggcagc
    20821 cagattgcca cgggtttcgc ccccctgcgg aatacgcgcc aggcagtacg gtaccggctc
    20881 gccatccacc accagcacgc gtttgtcgcc atctttaatg gctggcaggt aattttgcgc
    20941 catgcagtag cgagtgccat gctcagtcag ggtttcggca atcacgccga ggtttggatc
    21001 gccttctttc acgcggaaaa tcgacgcgcc gcccataccg tccagcggct taagaatgat
    21061 gtcgctgtgt ttctcccaga acgcttttag ctgcgcttta ttgcgcgtaa ccagcgtttc
    21121 tggcgttaag tcagagaacc aggcggtaaa cagtttctcg ttacagtcgc gcaggctctg
    21181 cggcttgtta acgatcagcg tccctttctc ttcggcacgt tccagaatat aggtcgcgta
    21241 gataaactcg gtatcaaacg gcgggtcttt acgcatcagg atcacatcga gatcggccag
    21301 cggcagatcc tgttcaccga cgaatgaaaa ccactcttcg tagttctgct tcacgttcag
    21361 cgtgcgggta tgggcgcggg cttcgccatt gatcagatac agatcgccca tctccatata
    21421 gtgaagttcg taaccacgac gctgtgcttc cagcaacata gcgaaactgg aatctttctt
    21481 gatgttgatg tttgcgatgg ggtccatcac gatgccgagc ttgatcatta ttcttctccg
    21541 ttagcccaaa tcgccaaatc gtacttgtag cgcggtaatg gcggtgagcg cagttgtctc
    21601 tgtacgcaaa acgcgaggtc ccaacaggat atcagtaaat tgatagcggg cagtcatggc
    21661 aatttcatct gccgataaac cgccttccgg gccaatcagc aggcggacgc gttcaaccgg
    21721 taaaggcaac gtattgatgc tgttactggc gcgcgggtga agattcagtt tcagtccttc
    21781 atcctgctct gcacaccagg cttccagatc catcgctgga cggatttccg gcactcggtt
    21841 acgaccacac tgctcacagg cagcaattgc aatcttctgc cactgctgga gcttcttgtt
    21901 cagacgttca ctatccagtt taacgccgca gcgctcagaa aaaagtggcg taatgaggct
    21961 tacaccgagt tcgatcgatt tctggatcgt aaattccatt ttttcaccac gcgacatcac
    22021 ctgaccgagg tgaatatgca gcggagattc gcgatcgtcg atctggcctt ccagcacctt
    22081 cacttccacg ctttttttgc tggcgctggt aatttcggcg tcaaagacct ggttgctgcc
    22141 gtcaaacaat tgcaacgcct gccccggccc catgcgcagt acgcgcccga tatggttggc
    22201 ggcatcttcg caaagcgcga tgtgagaatg gctggtcagt ggttcaggat gataaatgcg
    22261 ggggatacgc atagttaaaa atccgcgtcg tctccccacg cggttgtacg cgtggggtag
    22321 gggttaacaa aaagaatccc gctagtgtag gttagctctt tcgcgcctgg caagcgcgtt
    22381 gcacatacgg gttatgattg ccctgcacct tcgcgatgcg ttcatcgcgc tcgcactccc
    22441 agtcggtaac cggatacatc ttgttccatg cgttgaacag ctgcgtttgc tggcgagaga
    22501 gtgtcaggtt gtattggtca cgcatataga agtaggtgcg cgcaatggca ccgcgtgcac
    22561 gcgctggtgg ttcggcagct ttttctttga aatcgacctt catggcgcat tgaccgtact
    22621 ggccttcgcc gccattccac tggctgtaca taaagttgcc gcgatcgcca ttcacctcgc
    22681 cgactgacgg ctgcaggtta tgcatatcgc tttccatctt gcgatagacc ggatctttag
    22741 cgcagttttt acgtccaccg tcctgccagc actggcgctg gtgaccgaat tgccaggcgg
    22801 gaacgacgtg ttcccactct acgcggctgg cgcggttttc atttttgcgc acctgatagc
    22861 cgcacgattg cagatcaaca acgccttttt tgccctgcca gttaatttta catccgcaat
    22921 aaaacgtacc gggcgcgtca gcgtggactt ttaccgccgc ggctttcgcc tgagaaaaac
    22981 tattgatacc ttcggccaac gccgggccgg aaaatgctgc gctcagtacc accgcagcaa
    23041 tagacaaata acggtacatc acttactccg tgttaaaacg agccagcaac gtagcgaaag
    23101 ttgttttggt atgcaatcag ccagatcagg aaagttcctg atagttcaga tggttatttt
    23161 gcgaccagtt gttcgccgca gtgaacacag cgatagacgg cttcgccacg aactacgcga
    23221 ttatggcggc gtacggtaag ctgatgctcc tggcacttgc agcggtaggg gaaggtgttg
    23281 cgacgcacgg attgcagttc gaactgatgc gtacgacggg ctggaacgcc cagcacactt
    23341 tccatcatcc acttccactc tttgccatgt ggtgcaacgc ggccgaaatg tttccatacc
    23401 agcaaatgtg ccagttcgtg cggcaccact tcttcaataa aggcttcact gttttccaac
    23461 agcaaaacgg gattgaggcg gatttcatag ctttccagcc aggctgttcc ggcggaggtt
    23521 ccgcgctggg tgtaagagag ttttggctcc gggtagttac gccctagctt caggttggcc
    23581 tgggcgagtt tttcccgcag gcgacgcata acggcctgtt ggatggcgat agggagacgg
    23641 gatgttttca tagcggcaga ggatagagcg aagaaaagat gactgcaaga ggcggcttcc
    23701 tccgcgacgg gaggaagcct gaggagatta atcgtgagcg ccgatttcgc gcagtttacg
    23761 acctttcatc agattacgtt caatatgttc cagcgaaacg tgtttggttt ccggtaccag
    23821 ccacaatgtc agcaggataa acagtacgtt cagagccgca tacacccaga aggtgttggc
    23881 gttacccagc gtgttgagca tggtcaggaa cgttgcgcca acgatcatgt tggcaatcca
    23941 gttggtggcg gtggagcagg tgatgccaaa atcgcggcct ttcagcggct gaatttcgga
    24001 gcacagtacc caaatcagcg gaccggcact catggcaaaa ccgacaataa acatcagcag
    24061 catggcgatg gcgaaatact gcgccgacgg agagtggata ccgatatgca tcattgtacc
    24121 gagtacgccc ataccagcag ccatcaccag gaagcccagc gttagcgttg gtttacgtcc
    24181 ccagcggtca acaaggccga ttgcgataaa ggtggcaagt acgttggtca ggccgacaat
    24241 cactgtcccc cacatttgct cggtagtgtt ggtataaccc gccagttcga agattttcgg
    24301 cgcgtaatac atgatgacgt tcatcccggt gaattgctgc attacctgta acagtacgcc
    24361 aaggaacacc gcgcggcgga agttgctatt ctctttaaac agcgcccagc cactctgttt
    24421 aacctgcaaa ctttcacgga tttcatccag ttcgcgtttc gcttccgcgc tggtgtcacg
    24481 caggcgtagc agcacgcgtt cggcatcaac aaaacggcgt ttggcggcaa accaacgtgg
    24541 gctgtctggc aggaagaaga caccaatcag cagcaaaatt gccgggatga taatcacacc
    24601 cagcatccag cgccatgcac cggtgtagct gaaggcagta tcagaaagat aagcaccgag
    24661 gatcccgata gtgatcatca actgatacat cgagatcata ctgccgcgaa ttttttccgg
    24721 cgcaatttca gagaggtaca gtggtgcggt ataagaggcc acacctaccg ccagccccag
    24781 cagaacgcgg gaaagaatca gtacttcaac gtttggcgca gccgcagaga acagcgaacc
    24841 ggcaacaaac aaaattgcgc cgatcatcag gctctttttg cgcccgagtt taaaggagag
    24901 ccagccgctg cccaccgcac cgactgccgc accgaacatc atggagctta cgacccattc
    24961 ttgcgtgtgc gaagtaatct ggaattcatc tgcaataaac ggcagtgcgc cagcaattac
    25021 accgatatcc aggccaaaga gtaatcccgc cagagcggca aggaagcaga cgaaaaacgt
    25081 cattgccttg tttgaccgcc cctgtttttt agcgtcaggc atgatgccct ccaatatggt
    25141 tattttttat tgtgaattaa gataggtgag tacgacgtaa aaagatgtga agcaa
//
LOCUS       DAZUCE010000054        24962 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZUCE010000054 DAZUCE010000000
VERSION     DAZUCE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.054"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..673
                     /gene="espY1"
                     /locus_tag="ACHY03_001864"
     CDS             <1..673
                     /gene="espY1"
                     /locus_tag="ACHY03_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector EspY1"
                     /protein_id="HFS3905101.1"
                     /translation="KIDVKLNIFQRLWETLRHLFWSDKQTEAYKLLFNFVNKKAGNIN
                     ASKYFTGAVNENEKEKFINSLELFNELKTCAKNPDEMVAKGNMRWVAETYGDIELSVT
                     FFIENKEICTQTLQLHKGPGNLGVDLREAYLPGVDMRDCYFGLKTMKGHNKVLYLEPG
                     WNANLDGATLYGSILDGATVDGATHLYDEVIIINKITPKKIATEEIATKQSTAEEIIA
                     NAIIE"
     gene            complement(695..834)
                     /locus_tag="ACHY03_001865"
                     /pseudo
     CDS             complement(695..834)
                     /locus_tag="ACHY03_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
     gene            complement(1045..1704)
                     /gene="rluA"
                     /locus_tag="ACHY03_001866"
     CDS             complement(1045..1704)
                     /gene="rluA"
                     /locus_tag="ACHY03_001866"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFS3905102.1"
                     /translation="MRMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(1716..4622)
                     /gene="rapA"
                     /locus_tag="ACHY03_001867"
     CDS             complement(1716..4622)
                     /gene="rapA"
                     /locus_tag="ACHY03_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFS3905103.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLSEAQIEKSARALIDTARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            complement(4786..7137)
                     /gene="polB"
                     /locus_tag="ACHY03_001868"
     CDS             complement(4786..7137)
                     /gene="polB"
                     /locus_tag="ACHY03_001868"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFS3905104.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVFLAPQESVAF
                     IPADQVPRAQHILRGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGNASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNSGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            complement(7212..7907)
                     /gene="araD"
                     /locus_tag="ACHY03_001869"
     CDS             complement(7212..7907)
                     /gene="araD"
                     /locus_tag="ACHY03_001869"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFS3905105.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            complement(8076..9578)
                     /gene="araA"
                     /locus_tag="ACHY03_001870"
     CDS             complement(8076..9578)
                     /gene="araA"
                     /locus_tag="ACHY03_001870"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFS3905106.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVKALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(9589..11289)
                     /gene="araB"
                     /locus_tag="ACHY03_001871"
     CDS             complement(9589..11289)
                     /gene="araB"
                     /locus_tag="ACHY03_001871"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFS3905107.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            11628..12506
                     /gene="araC"
                     /locus_tag="ACHY03_001872"
     CDS             11628..12506
                     /gene="araC"
                     /locus_tag="ACHY03_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFS3905108.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESIHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            12592..13356
                     /gene="yabI"
                     /locus_tag="ACHY03_001873"
     CDS             12592..13356
                     /gene="yabI"
                     /locus_tag="ACHY03_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFS3905109.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPIAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(13443..14141)
                     /gene="thiQ"
                     /locus_tag="ACHY03_001874"
     CDS             complement(13443..14141)
                     /gene="thiQ"
                     /locus_tag="ACHY03_001874"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFS3905110.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTEELL
                     SGKASASALLGITG"
     gene            complement(14125..15735)
                     /gene="thiP"
                     /locus_tag="ACHY03_001875"
     CDS             complement(14125..15735)
                     /gene="thiP"
                     /locus_tag="ACHY03_001875"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFS3905111.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVIAVALAAFLALWSNAPQSDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRKLRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNSTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(15711..16694)
                     /gene="thiB"
                     /locus_tag="ACHY03_001876"
     CDS             complement(15711..16694)
                     /gene="thiB"
                     /locus_tag="ACHY03_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFS3905112.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAINVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(16725..16824)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            17066..17635
                     /gene="espY2"
                     /locus_tag="ACHY03_001877"
     CDS             17066..17635
                     /gene="espY2"
                     /locus_tag="ACHY03_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HFS3905113.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRIVNLVQKTENMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKARDQLTHWLIK
                     FNELKEHARDPENMAAKASLSPKDTLCVSFFIGDEAIFTLKLQLKENTRTGCIDLSNA
                     YFNGVVICGIDCLEVDMSNAETNNSRWYD"
     gene            complement(17864..19522)
                     /gene="sgrR"
                     /locus_tag="ACHY03_001878"
     CDS             complement(17864..19522)
                     /gene="sgrR"
                     /locus_tag="ACHY03_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFS3905114.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            19611..19742
                     /gene="sgrT"
                     /locus_tag="ACHY03_001879"
     CDS             19611..19742
                     /gene="sgrT"
                     /locus_tag="ACHY03_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFS3905115.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(19850..20455)
                     /gene="leuD"
                     /locus_tag="ACHY03_001880"
     CDS             complement(19850..20455)
                     /gene="leuD"
                     /locus_tag="ACHY03_001880"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFS3905116.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCLMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(20466..21866)
                     /gene="leuC"
                     /locus_tag="ACHY03_001881"
     CDS             complement(20466..21866)
                     /gene="leuC"
                     /locus_tag="ACHY03_001881"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFS3905117.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(21869..22960)
                     /gene="leuB"
                     /locus_tag="ACHY03_001882"
     CDS             complement(21869..22960)
                     /gene="leuB"
                     /locus_tag="ACHY03_001882"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFS3905118.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPETVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(22960..24531)
                     /gene="leuA"
                     /locus_tag="ACHY03_001883"
     CDS             complement(22960..24531)
                     /gene="leuA"
                     /locus_tag="ACHY03_001883"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFS3905119.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(24624..24710)
                     /gene="leuL"
                     /locus_tag="ACHY03_001884"
     CDS             complement(24624..24710)
                     /gene="leuL"
                     /locus_tag="ACHY03_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFS3905120.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         5679 a         6702 c         6730 g         5851 t
ORIGIN      
        1 caagattgac gtgaagctga atatctttca acgcttgtgg gagacgttac gtcatctgtt
       61 ctggtcagat aaacagactg aggcttataa acttctgttc aatttcgtga ataaaaaggc
      121 tggcaacatc aacgcctcaa aatactttac tggggctgtc aacgagaatg agaaagaaaa
      181 gtttatcaat agcctggaat tattcaatga actcaaaaca tgcgcaaaaa atccggatga
      241 gatggtcgca aagggcaata tgcgctgggt cgccgagacc tacggtgata tcgagttaag
      301 tgtcactttt ttcattgaaa ataaggagat atgtactcag acgttgcagc tgcataaagg
      361 cccaggtaac ttgggcgttg atcttagaga ggcttacctt cccggcgttg acatgaggga
      421 ttgttacttt ggtctcaaaa caatgaaagg tcacaataaa gtcctttact tggaacctgg
      481 gtggaatgct aacctggacg gtgctaccct gtacggttct atcctggacg gtgctaccgt
      541 ggacggtgct acccacctat atgatgaggt aattattatt aataaaatca ctcccaaaaa
      601 aattgctacc gaagaaattg ctactaaaca aagtactgct gaagaaatta ttgccaacgc
      661 aattattgaa tgatccagaa taagctaagg ttgatttcac aattagaaag acctgcatga
      721 tgagcgagag aagaggctag tgacgcaagg cgtcgtgcac gacgcggatc accgagatgg
      781 gcatcgccaa ccagactgct aattagccca tgaataacaa tcagaaagga ccataacaga
      841 cccgataaaa tgaaatagta gagacgatca acgggtgaag aaaaagttca aaaattcgct
      901 gtggagcagg aagggaatta ccaaatggaa agcgtagcca cacgcaacaa ctgaaagcag
      961 tttggcagaa aaaaaatccc cggactcggg gatttatgta caagaggcag cccttaggat
     1021 gagggtataa acgtacagga aaggttaaaa atccgctggc gctttaaacg tcatactatt
     1081 gccatacgcc ggatgggtaa tcgtcaacat ctctgcatgt agcaacaaac gcggtgccat
     1141 cgctctcgct tccggtgatg cataaaaacg atcgccgaga atcggatgac ccagcgccag
     1201 catatgcaca cgcaattgat gcgaacgccc ggtaatcggt tttaacacca ctcttgccgt
     1261 gttatccgcc gcatactcca ccacttcata ttccgtctgc gcaggtttac ctgtttcgta
     1321 acagactttc tgtttcgggc ggtttggcca gtcgcaaatc agcggcagat ctaccagacc
     1381 ttccgcaggg gatggatgcc cccagacgcg ggccacatac tgctttttcg gctcgcgctc
     1441 gcggaactgg cgttttaact cccgctccgc ggctttggtc agcgccacta caatcacgcc
     1501 gctggtagcc atatccagac gatgcaccga ttctgcctgc ggataatcac gctgaatgcg
     1561 cgtcatcacg ctgtctttgt gctcttccag acgacccggc actgacaaca aaccgctcgg
     1621 cttgttgacc accataatat ggtcatcctg atacaggata accaaccagg gttcctgcgg
     1681 tggattgtag ttttccatcc tcattttcgg ctccgttact gatgcgtcac aacgatcaaa
     1741 cgcagggcat ccagacgcca gcctgcctga tccaggcttt ccattacctg ctgacggttg
     1801 ctctcaatgg cggtcagttc gtcgtcacga atgttcgggt tcactgcgcg cagcgcttcc
     1861 agacgagaca gctcggcaga cagtttttcg tcggcttcgt tacgtgcggt atcaatcaat
     1921 gcacgggccg atttctcgat ctgcgcttca ctcagctgaa ggatagcgtg aacatcctgc
     1981 tgcacggcat taaccagttt gctgccggtg tgacggttga ccgcgttaag ctggcggtta
     2041 aaggtttcaa actccacctg ggccgccagg ttgttgccat ttttatccag cagcatacgt
     2101 accggcgtcg gtggcaggaa gcggttgagc tgcaactgct tcggcgcctg ggcttcgacc
     2161 acgtaaatca gttccaccaa cagcgtacct accggtaacg ctttgttttt caacagtgaa
     2221 atcgtgctgc taccagtatc gccagaaagg atcagatcca gaccgttgcg gatcagcgga
     2281 tgctcccagg taataaactg tgcatcttca cgcgccagcg ccacttcacg atcaaaggtg
     2341 atggtgatgc catcttccga caggccaggg aagtccggca ccagcatatg atcggacggc
     2401 gtcagcacga tcatgttgtc gccgcgatcg tcctgattga taccgataat atcgaacagg
     2461 ttcatggcga aggcgatcag gttggtatcg tcatcctgct cttcaatgct ttctgccagc
     2521 gcctgggctt tttcgccacc gttggagtgg atttccagca ggcggtcacg accctgttcc
     2581 agctgtgctt tcagcgcttc atgctgctcg cggcagtttt tgatcaaatc gtcaaagcct
     2641 tcggtttgat ccggtgaagc cagatagtta atcagatcgt tgtatacgct atcgtaaata
     2701 gtgcgtccgg tcgggcaggt gtgttcaaat gcatccagac cttcgtgata ccagcgcacc
     2761 agcaccgact gagcagtttt ttccagataa ggcacatgga tctgaatatc gtgcgcctgg
     2821 ccgatacgat ccagacgacc aatacgctgc tccagcagat ccgggttgaa tggcaggtca
     2881 aacatcacca tgtggctggc gaactggaag ttacgtcctt cagaaccgat ttccgagcac
     2941 agcagtacct gtgcgccggt gtcttcttcg gcaaaccagg cggcagcgcg gtcacgctcg
     3001 ataatcgaca taccttcgtg gaacaccgca gcgcgaatac cttcacgttc gcgcagtacc
     3061 tgctccagtt gcagcgcggt agcggcttta gcgcagatca ccagcacttt ctgagagcga
     3121 tggctggtca ggtagcccat cagccactca acgcgcggat cgaagttcca ccaggtggcg
     3181 ttatcacctt caaattcctg ataaatacgc tccgggtaaa gcatatcgcg ggcgcggtct
     3241 tccgcacttt tacgtgcgcc cataatgccg gagactttaa tagccgtctg atactgcgtc
     3301 ggcagcggca gcttaatggt gtgcagctcg cgtttcggga agcctttcac accgttacgg
     3361 gtgttacgga acagcacgcg gctggtgccg tggcgatcca tcagcatcga aaccagctcc
     3421 tgacgggcgc tctgggcatc ttcgctgtcg ctgtttgctg cttgcaacag cggctcgata
     3481 tcctgctcgc cgatcatctc gccaagcatg ttcagttcgt cattgctcag tttgttacct
     3541 gccagcagca tggcaacggc gtccgcaacc ggacgataat ttttctgctc ttcaacgaac
     3601 tgcgcaaaat cgtggaaacg gttcgggtcc agcagacgca gacgggcgaa gtggctttcc
     3661 atccccagct gttccggggt cgcggtcagc agcagaacgc ccggcacgtg ctctgccagt
     3721 tgttcaatgg cctgatattc acggcttggc gcatcttcgc tccacaccag gtgatgcgct
     3781 tcatcgacca ccagcaggtc ccattcggct tcacagagat gttccaggcg ctgtttgcta
     3841 cgacgggcaa aatccagcga gcaaatcacc agctgttcgg tgtcgaacgg gttgtaagca
     3901 tcgtgctgag cttcggcata acgctcatca tcaaacagcg caaagcgcag gttgaaacgg
     3961 cgcagcattt ctaccagcca ctgatgctgt aaggtttccg ggacgataat cagcacacgt
     4021 tcagcagcgc cagagagcag ttgctgatga aggatcatcc cggcttcaat ggttttccct
     4081 aaacccactt cgtcagccag caggacgcgc ggcgcgtggc ggcggccaac atcatgagcg
     4141 atgttgagct ggtgcgggat caggctggta cgctgaccgc gcaggccgct gtacggcata
     4201 cggaactgtt cgctggaata cttacgcgcg cgataacgca gcgcaaagcg gtccatacgg
     4261 tcaatctgcc cggcaaacag gcggtcctgc ggtttgctga acaccagttt gctatcaagg
     4321 aaaacttcac gcagggctac gccggactct tcagtatcca ggcgagtacc gatataggtc
     4381 agcaagccat tttcttcttt tacttcttcg acttgcatct gccagccgtc atggctggta
     4441 atggtatcac cagggttgaa catcacgcgg gtcacggggg aatcactgcg tgcgtacaga
     4501 cggttttcac cagtagatgg gaaaagtaaa gtgacagttc gcgcatccac cgcgacaacg
     4561 gttccaagtc ccaattcgct ttctgtatcg ctgatccagc gttgaccaag tgtaaaaggc
     4621 atatgtgttc ggctctatat ctttaattgc aggcaataac cacccgctac cgtgcttatg
     4681 aggtagtggt gttattcagg tccaggaatg gaaagggcgc tatggtactg gatggcaaag
     4741 cattcgtcac gcatcaaaat ggtatctggc gaactctttt tttgctcaaa atagcccaag
     4801 ttgcccggtc ataagtgtag caaaattatc ctcaataaaa gggagtattc cctccgccac
     4861 gggttgtagc tggcgggtca gatagtgttc gtaatccagt ggtgaacgtt ggtagtccag
     4921 cggctccggg ccgttggtgg tccatacgta cttaatggtg ccgctgttct gatattgcaa
     4981 ggggcgaccg cgcttttggt tttcttcatc ggcaagacga gcggcgcgta catgaggcgg
     5041 aacattacgc tgatattcgc tcagcggacg gcgaaggcgt ttacggtaaa ccagccgcgc
     5101 atccagttca cccgccatca gtttgtcgat ggtttcgcgg acgtactcct gatatggctc
     5161 gttgcggaag atacgcaggt aaagctcctg ctgaaactgc tgggccagcg gcgtccagtc
     5221 cgtacgcacg gtttccagcc ctttaaacac catccgctgt ttgtcgccct cctgaatcag
     5281 cccggcataa cgctttttac tgccggtatc ggctccgcga atggttggca tcagaaaacg
     5341 gcagaaatgg gtttcatact ccagttccag tgcgctggtc agctgttgtt tttgcagcgt
     5401 ttccgcccac caggcgttaa cgtgctgcac cagtacacga ccgattttcg ccgcttcttc
     5461 ttccgaatgt gcgcctttca gccagacaaa cgttgagtcg gtatcgccgt agatcacgtc
     5521 gtagccctgc gcttcaatca acgctttggt ttgccgcatg atctgatgac cacgcatggt
     5581 gatcgacgat gccagccgcg gatcgaagaa gcggcaggcg gtggtgccga gcacgccata
     5641 aaaggcattc atgatgattt tcagcgcctg cgacagcggc ttattaccct gacgtttggc
     5701 ttcatcgcgc ccgtgccaga tgttagtcac aatctccggc aggcaatgtt tttctcgcga
     5761 gaaccaggca tcgagaaagc cttcggtgct gtgctctgga tcaggctgcg ccatgccttc
     5821 caccagcccg acgggatcaa tcagaaaggt gcggatgatc gacgggtaca ggcttttgta
     5881 gtccagcacc agcaccgaat cataaagccc aggtcgcgaa tccatcacgt agccgccagg
     5941 gctggcgtgc ggcggcactt cgccgagatt aggcgcgaca taaccagcgc gatgcatccg
     6001 gggaaaatag agatgaccaa acgccgccac cgatcccccg tggcggtcta ccggcaggcc
     6061 gttcaccgtt gctcgttcga gcaaaaacgg catgatttca gttttatgga aaatctgcgt
     6121 caccagctcg caatctttca ggttataagt cgccagcgca ggtttatctt cggcgaaacg
     6181 gcggtcaatt tcgtccattc gatcccacgg gttatcgata gattttcctt cgcctaatag
     6241 ctcctgagcg acagtttcca gcgagaatga agagaaattc cagaacgcgg atttcagcgc
     6301 ctcgataccg tcgataatta gccgcccttt agcctgggca aaaaagacgc cgtttttaaa
     6361 gccgtgctcg cgccactcca gctcgctgtt atcgcgcccc agccgcagcg gaatacgata
     6421 acgctcggca tgtttttgca gcattcgcag atcgaattgc accacgttcc aaccgatgat
     6481 cacatcagga tcgtagttgg caaaccaggc gttaagtttt tccagtagct gcgggcgact
     6541 ggcgacgtat tccagttcga aatcgagcgc ggaagcgttg ccattctccg gccccagcat
     6601 ataaacgatg cgctgcccgc agccctccag gccgatgcag tacagctcac cgtggcgggt
     6661 ggtttcaata tcaatagaca cccacttgag cggcgggcga tagtcgggat gcggtttcag
     6721 acgggcatta acgatagcgc cattgtgcat atcaccctcg acccacaccg gcgaggtgat
     6781 aaatcgctcc atcagatagc gttctggcgg gcgcacatcg gcctcgtaga cggtaacgcc
     6841 accttcacgc aggcgctttt cgtaattcat caactggcga tgggcgcgac agtaaaggcc
     6901 atacaccggc tggcggtgaa aatcctttaa cgccagcggc gtcaggcgaa agccttgttc
     6961 accccgcaaa atatgctgag cgcggggaac ctgatcggcg ggaataaacg ccacggactc
     7021 ttgcggcgca aggaaaacct gcaacggccc gttgtccgtc gccagccaga aggagacctc
     7081 tgttccttgc ggggtatccc gccagtgtcg ggttaagata aaacctgcct gcgccacgct
     7141 gaaaatccat caaaaaacca ggcttgatta tagcctggtt tcatttgatt ggctgtggtt
     7201 ttatacagtc attactgccc gtaatatgcc ttcgcgccat gcttacgcag atagtgttta
     7261 tccagcagcg tttgctgcat atccggtaac tgcggcgcta actgacggca gaatatcccc
     7321 atataagcga cctcttccag cacgatggcg ttatgcaccg catcttcggc atttttgccc
     7381 catgcaaatg ggccgtgaga atggaccaga acgccgggca tttgcgctgc atcgataccc
     7441 tgtttttcga aggtttctac gatgacgtta ccggtttccc actcatattc gccgttgatt
     7501 tctgcgtcgg tcattttgcg ggtgcaggga atggtgccgt agaaatagtc ggcgtgggtg
     7561 gtaccagttg ctggaatcga ctgacccgcc tgcgcccaga tggtggcgtg gcgcgagtgt
     7621 gtgtgcacaa tgccgccaat ggacgggaat gcctgataga gcagccggtg agttggcgtg
     7681 tcggaggagg gctttttcgt accttcaacc acttcaccgg tttcgatgct gaccacgacc
     7741 atatcgtcgg cggtcatgat gctgtaatcg acgccggaag gtttgatcac aaagacgccg
     7801 cgctcgcgat caacggcgct gacgttgccc caggtgagcg tgaccaggtt atgttttggc
     7861 agcgccaaat tggcttctaa tacctggcgt ttgagatctt ctaacatgtt gactcctgag
     7921 aggtcgtttc ccctcacccc ggccctctcc ccaaaggggc gagggggaaa acaatgccgg
     7981 atgcgctttg cttatccggc ctacaaaatc acagcgtgta ggcctgataa gacgcgtcag
     8041 cgtcgcatca ggcgttacac acctgatgcg gctacttagc gacgaaatcc gtaatacact
     8101 tcgttccagc gcagcgcgtc tttaaacgct ggcaggcggg tgtcgttatc aatcaccgtg
     8161 atttcaatgt cgtgcatctc ggcgaactgg cgcatatcgt tgaggttcag cgcatggctg
     8221 aagacggtat ggtgcgcgcc accagcgagg atccacgctt cggaagcagt tggcagatcc
     8281 ggttgcgctt tccacagcgc attcgccacc ggcagtttcg gcagggagtg cggtgttttc
     8341 accgtgtcga tgcagttaac cagtagacgg taacgatcgc cgagatcaat caggctggcg
     8401 acaatcgctg ggccggtttg ggtattgaag atcaggcggg caggatcgtc cttaccacca
     8461 ataccgagat gctgaacgtc gaggatcggt ttctcttctg cggcgatcga cgggcagact
     8521 tccagcatgt gggagccgag caccaggtca ttgcctttct cgaagtgata ggtgtagtcc
     8581 tccataaagg aggtgccgcc ctgcagaccg gttgacatca ccttcatgat gcgaagcagg
     8641 gcggcggttt tccagtcgcc ttcgcccgca aagccgtaac cctgctgcat cagacgctgt
     8701 acggccagac ctggaagctg tttcagaccg tgcaaatctt caaaggtggt ggtgaacgcg
     8761 tggaagccac cttgttccag gaaacgtttc atccccagct caatacgcgc cgcttccagc
     8821 acgttctgtc gtttttcgcc gtggatttgt gtggcaggcg tcatggtgta gcagctttcg
     8881 tactcatcga ccagcgcgtt aacatcgccg tcgctgatgg agttcaccac ctgcaccaga
     8941 tcgccaaccg cccaggtatt gacggagaaa ccgaacttga tttgtgcggc aactttatcg
     9001 ccatcggtga ccgccacttc acgcatgtta tcgccaaaac ggcagacttt cagatgacgg
     9061 gtatcctgtt tagagaccgc ctgacgcatc caggagccga tacgctcatg ggcttgttta
     9121 tcctgccagt gaccggtaac gacggcatgt tgctgacgca tacgcgcgcc aatgaagccg
     9181 aactcgcgac cgccgtgcgc ggtctggttc aggttcataa agtccatatc gatactgtcc
     9241 cacggcagcg ccgcgttgaa ctgggtgtgg aattgcagca acggtttgtt gagcatggtc
     9301 aggccgttga tccacatttt ggccggggag aaggtgtgca gccacaccac cagaccagcg
     9361 caacgatcgt cgtaattcgc gtcgcggcaa atagcggtga tttcatccgg cgtggtgccc
     9421 agcggtttca gtaccagttt gcagggcagt ttcgcttccg tattcagcgc tttcacaacg
     9481 tgctcggcat gttgggtgac ctgacgcagg gtttccgggc catacagatg ctggctgcca
     9541 attacaaacc acacttcata attatcaaaa atcgtcatta tcgtgtcctt atagagtcgg
     9601 aacggcctgg gcagcctgtg ccggggcgga agttggaaga tagtgttgtt cggcgctcat
     9661 cgcccattgc tgatagcggc gataaagctg ttcaaagcgt tgtgcctgtt cgctgcgcgg
     9721 ttgcagggtt ttctctaccg cactggccat tttttgctgg gctgatggga tgtctgcgtg
     9781 cactttcgcg gcgacggcag caaaaatcgc cgcaccgagc gcacagcact ggtcagaggc
     9841 aacaatttgc agcgggcgat tcagcacgtc gcagcaggcc tgcataatga cctggttttt
     9901 ccgcgcgatg ccgcccagcg ccatcacgtt attgacggcg atcccctgat cggtaaagca
     9961 ctccatgatt gcgcgtgcgc caaaggcggt ggcagcaatc aaaccgccga acagcagcgg
    10021 agcgtcggta gcgaggttaa gatcggtaat cacccctttc aggcgttggt tagcgttcgg
    10081 tgtgcggcgg ccgttaaacc agtcgagcac caccggcagg tgatccagag acggattttt
    10141 ggcccatgct tcggtcagcg ccggaagcag ttgtttctgg ctggcgttga tttgctcttt
    10201 tagttccgga tgctgggcgg caagctgttc cagcggccag ccgagtacgc gaccgaacca
    10261 ggcgtagata tcaccaaacg ccgattggcc tgcttccaga ccgataaatc caggcaccac
    10321 gctgccatca acctgaccgc aaataccttt cactgcccgc tcgccaacgc tctgtttgtc
    10381 ggcaatcaga atgtcgcagg tggaagtacc gataactttt accagtgcgt taggctgtgc
    10441 gcctgcgcca actgcgccca tatggcagtc aaacgcgccg ccggaaatca ccacgctttc
    10501 aggcaggccg agacgctgcg cccattccgg gcataaggtg cccaccggaa tatcggcagt
    10561 ccaggtgtca gtgaacagcg gggaaggcaa atggcgattg aggatcgggt ccagctcatc
    10621 aaagaaactg gctggcggca ggccgcccca gctttcgtgc cacagagatt tatgcccggc
    10681 gctgcaacgt ccgcgacgaa tatcctgcgg gcgggtggta ccggaaagca gagctggcac
    10741 ccagtcgcac agctcaatcc acgatgcggc agattgcgcc acggcgctgt cctggcgagt
    10801 cacatgcagg atttttgccc agaaccattc gctggaataa ataccgccaa tatagcggga
    10861 gtagtcaacg ttgcccggcg cgtggcacaa acgggtaatc tcttccgctt cttcaaccgc
    10921 agtgtggtct ttccacaata cgaacatcgc gttcgggttt tcggcaaact ccgggcgcag
    10981 cgccagcacg ttaccgtcgg catcaatcgg tgcgggcgtc gagccggtac tgtcaacgcc
    11041 aatcccgacc acagctgcgc gctgttcgac gctaagctct gcaagcacgg ttttcagtgc
    11101 cgcttccatt gactcaatgt agtcacgcgg atgatgacgg aactggttat tcggggcatc
    11161 acaaaattgc cctttttgcc aacggggata ccactctacg ctggtggcga tctcttcacc
    11221 gctggcgcag tccaccgcca aagctcgcac agaatcactg ccaaaatcga ggccaattgc
    11281 aatcgccatc gtttcactcc atccaaaaaa acgggtatgg agaaacagta gagagttgcg
    11341 ataaaaagcg tcaggtagga tccgctaatc ttatggataa aaatgctatg gcatagcaaa
    11401 gtgtgacgcc gtgcaaataa tcaatgtgga cttttctgcc gtgattatag acacttttgt
    11461 tacgcgtttt tgtcatggct ttggtcccgc tttgttacag aatgctttta ataagcgggg
    11521 ttaccggttg ggttagcgag aagagccagt aaaagacgca gtgacggcaa tatctgatgc
    11581 aatatggaca attggtttct tctctgaatg gcgggagtat gaaaagtatg gctgaagcgc
    11641 aaaatgatcc cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc
    11701 cgattgaggc caacggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt
    11761 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg
    11821 tctgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc
    11881 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc
    11941 atgaatggct taactggcca tcaatatttg ccaatacggg tttctttcgc ccggatgaag
    12001 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag
    12061 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg
    12121 aagcgattaa cgagtcgatc catccaccga tggataatcg ggtacgcgag gcttgtcagt
    12181 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt
    12241 gcttgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa
    12301 gctggcgcga ggaccaacgc atcagccagg cgaagctgct tttgagcacc acccggatgc
    12361 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat
    12421 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga
    12481 atgatgtagc cgtcaagttg tcataattgg taacgaatca gacaattgac ggcttgacgg
    12541 agtagcatag ggtttgcaga atccctgctt cgtccatttg acaggcacat tatgcaagca
    12601 ttgctggaac actttattac ccaatccacc gtgtattcat tgatggcggt ggtgttggtg
    12661 gcctttctgg agtcgctggc gctggtcggt ttgattctac ccggtacggt gctgatggcg
    12721 gggctgggag cgctgattgg cagcggcgag ttaagtttct ggcacgcctg gctggcaggg
    12781 atcgtcggtt gtctgctggg cgactggatc tccttctggc tgggctggcg ttttaaaaag
    12841 ccgttgcatc gctggtcatt tctgaagaaa aacaaagcat tactcgataa aactgaacat
    12901 gcgttgcatc aacacagcat gttcaccatt ctggtcggtc gctttgttgg cccgacgcgt
    12961 ccgctggtgc caatggtggc gggaatgctg gatctgccga tagcgaaatt tatcacgccg
    13021 aatattatcg gctgcctgct gtggccgccg ttttacttcc tgccggggat tctggcgggg
    13081 gcggcgatcg atattcctgc cggaatgcag agtggcgagt ttaaatggtt gctgctggcg
    13141 acggcggtat ttttgtgggt tggtggctgg ctgtgctggc ggttatggcg cagtggtaaa
    13201 gcgactgacc gtttgagtca ttatttgtcc cgcggtcgtt tgttgtggct gacgccgttg
    13261 atttctgcca tcggcgtggt ggcgctggtg gtgttaattc gccatccgtt aatgccggtg
    13321 tatatcgata ttttgcgtaa agtggttgga gtttagggca tagtcttgtg tggggcgctt
    13381 gatgcggaca aaacgcaccc gtaggtcgga taaggcgttc acgccgcatc cgacagtgca
    13441 tattaacctg taattcccaa cagcgccgaa gcactcgcct taccgctcaa caattcttcg
    13501 gtcataccct gccaggcgat gcgcccgtcg gcgactacta ccgagcgcgt ggcgatccgc
    13561 gccgcatctt ccacgctgtg cgacaccatc aatagcgtca ttttttgctg ctggcagctc
    13621 gtgctcacca gcgtcaacat ctcctgacgt aacgccggat cgagcgcaga gaacggttca
    13681 tcgagcaata aaatcggctg ttcgcgtacc agacaacgcg ctaacgccac tcgctgtcgc
    13741 tgaccgccgg aaagctcgcc aggtaaccgc gccattaaat tatcaatccc catctgacgg
    13801 gcaatcgcgt gcattttctc ctgctgtgcc gcgttcagtt tcaatcccgg atttagcccc
    13861 agcccgatgt tctgcgcgac cgtcaggtgg ctgaacaggt tgttctcctg aaacagcatc
    13921 gacaccggac ggcgtgacgg cggcgtcgtg gtgtgatcaa cgccatcgat agtcagcgaa
    13981 ccgctggctg gcgtcagaaa accggcaatc aaattcagca gggtactttt acctgcgccg
    14041 cttggcccga ggatcgccac ctgctcgccg cgttctactg ttaagctaaa acgcatcggc
    14101 aaatggtggt aaagccaggt gatatcagtc agttttaaca tttcgccccg gtagtttttc
    14161 aatcacggta aacagcagaa aacagagtag cagcagaatt aacgcggtga ccgcgccgtc
    14221 ctggctgcga taggagccaa tttgctggta gagataaaac ggcagggtgc ggaaatcatc
    14281 gttaccgaac aacgctacca cgccaaaatc accaatcgac agcacgcagg caaaggccag
    14341 cgcctgcgcc agtggacgtt tcagggcgcg cagctccacc acttttaagc gtgaccagcc
    14401 ttcaatcccc agcgactgac ataacatgct gtagcgggcg gtgatatcgc gcatcgggtt
    14461 ttccagcact ttcagggcgt aggggatcgc cattaacgca ttggtgaaaa tcacaatgcc
    14521 gtcagcagat tgcggcaggc caatggtgct gttgagcagt aaaaagaagc ccgttgccag
    14581 cacaatcccc ggcatagcga ggatcaacat gccgctcatc tccagcgcct ggcccgccag
    14641 cattttttgc cgcgcccgaa gtttgcgact gctccatagc agcatcatgg tcagcactac
    14701 gcacaatacg cctgccgcca gcgcaatacg caacgaagtc cacagcgcct gccacagcac
    14761 cggttgtgcc agcacttccg gcaactggcg atttacccca tcgacgatca ccgccagtaa
    14821 cggtggaagc aacagcagca gcgccagcac aattaacacc gtgtcgcaaa tgcggctatg
    14881 cagacgatcg tccgggtcgc gccagccttg cagcagcgtg gtgccgggcg caatggcctt
    14941 actcagtcgc tgactcaaca gcaccagccc aaggcaacac accatctgga tcagcgccag
    15001 catcgccgcg cgggcaggat cgtagtcgta actcagcgcc tgatagattg ccagctcgat
    15061 agtggtcgcc tgcggaccgc cgcccagcga cagtacggtg gcgaagctgg cgaaacagag
    15121 cataaagata agcgcagcaa ccggcgggat ttgtctccgt aaccacggcc attcgacgaa
    15181 gcggaaaaaa tgccagccgc gcatcccaag ctgggcggca agctgacgtt gttcgccggg
    15241 aatgttttcc agtgcctgga gtaataagcg gctcgccatc ggtagattaa aaaatacgtg
    15301 cgccagcaaa ataccttgca ggccgtaggg cgaaaaggtc cactccagac cgagcgattg
    15361 gcagagcgat gccagccagc cctggcgacc atagacgcta agaatgccga aaacagcaac
    15421 cagcaccggg aggatcaagg tcattgcgca cagacgcaac agcgccagcc gacccggaaa
    15481 gcgcctgcga tagagcgcgc gggcgaggaa tatcgcgggt acgacagaga gtagcgccga
    15541 gagaaacgcc tgccagaagg agaagcgcac cacatgccac aggtagctgt cctgccagac
    15601 tgccgaccag tcactctgcg gcgcgttcga ccacagggcg agaaacgccg ccagcgcaac
    15661 cgctatcacc agcgtggcgg cgcttacacc tggaattaac cagccgggaa ttaacggctg
    15721 acggcgcgtt gccattcgct aatccatgcc tgacgttgtg ccgccacttc ggctggcgtg
    15781 aactccagcg tggttgcggg tttggtcaat ttttcaaaac cggcaggcag cgtgacgttt
    15841 gccaccggat acatccagtt gccggttggg atcgcattct ggaaagccgg agaaaccata
    15901 aactggagga atttttgcgc cagctccggc tgcttgctgg cagcggtgcg agcggcgact
    15961 tccacttgca gatagtgacc ttcgctgaag ttcgcggcgg cgtagttatc tttcttctct
    16021 tcgagaatgt gataagccgg agaggtggtg taactcagta ccagatcgct ttcacctttt
    16081 aaaaacaggc cgtaggcttc gctccagcct ttggtgacgg tgaccgtttt cttcgccagt
    16141 ttctgccagg cttgtggggc gtcatcgcca tagacttttt gcatccatag caacagaccc
    16201 agccccggtg tactggtgcg cggatcctga taaatcaccc gccagttttg atcgctctca
    16261 accagttctt tcaggctttg tggcgggttt ttcagtttgt tcttgtcata aacgaaggcg
    16321 aagtagccat aatcaaacgg tacgaaagtg tcattattcc agccgccggg aacgttaatg
    16381 gcatccgctg ccacaccgct tttggcaaac agtccggttt tactggcggc gtctaacagg
    16441 ttgttatcca gccccagcac cacatcggct ttactgtttt tgccttccat ccgtagacgg
    16501 ttgagaagcg aaacgccatc ttccagcgcc accagtttca gttcgcaatt acagtcggct
    16561 tcaaaggctt ttttaaccac cggaccaggc ccccagtcgg cggcgaagga atcgtaggta
    16621 taaacagtca gaacgggttt agcgaaaacg ggcgctgtgc acagcaacag caggggaaga
    16681 cattttttta acactttgca cctcaaaaaa gagtggcaaa ggacttgaga aggagcctca
    16741 aatcccttcg ccggcgttat ccggatcagg ttcgacgggt attttctcag cgcacgcgtc
    16801 cgcgtggcac cccgttgaga acggcgttag tgtagtgatt ttgttatcaa ccagcaatcc
    16861 atagagaagg cgtattaaaa aagccaatat tcgttcagct atcaagagcg tatttgcata
    16921 gctggagagc gttgatccga aagaatccct tagctacagt cagaataggt aacgtaaccg
    16981 tttattgcgt gaatgagcga tttttgacag tattcttatt caggacgctt attctgagtt
    17041 ttttactcag agtggagcta tcagcatgaa agtaagaaac ccagaacaga ttagtatccc
    17101 tgctagtaat accactaaag atccaggcct tactaattct cagattatca ggatagttaa
    17161 cttggtccaa aagaccgaga atatgaatat ctttgaagag ctatgggaga cgttacgcaa
    17221 tctctttcgg tcagataaac attctcagac tgcggcaagg caaattctga aggatgcatt
    17281 ttatttccag aatagtgatg actactcaaa atactttacc ggggctgtcg acgggaaggc
    17341 gcgtgatcag ttaactcact ggctgataaa atttaatgaa ctcaaagaac acgcaagaga
    17401 tccggaaaat atggccgcga aggcctcact ctcgccaaaa gatacgttat gcgtcagttt
    17461 ttttattggc gatgaggcga tatttactct gaagttgcag ctgaaagaga acaccaggac
    17521 cgggtgcatt gacctgagta atgcttactt taacggtgtt gtcatttgtg gtattgactg
    17581 tctcgaggtt gacatgagca atgctgaaac gaacaatagc agatggtatg actgacctgc
    17641 gcgaggttaa cctgaaaaag atgatctttc cggccagaac aaggctagac tgcgccgtta
    17701 ttgattaact cagaatatga aagaggggct ggaatgcccc ttctttaatc ttatttgcga
    17761 cgctaatcag acgatgcata aaacagagaa aatataaaag taatgtattt tatcattccg
    17821 tctgaatata aaaaatgcgc tcatgaccgc caaaaggcgc aagctactct ggatccggcg
    17881 gcgcaaacca cgctgattta aaatcgaacc agccgagggt gttcatgcgt aggccgcgca
    17941 tactgcgttg cccctgaatg atcagccagt ggtggataag tggcaccatt gctttactgg
    18001 cgaccagttg ctggcaccag ttcgccagat tcatctcgcc attgcgccag cgggcggcgt
    18061 cggcttgcca gtcgatcgga atacagtgtt ggagcagcgg cacctcgcac aggtgcgcga
    18121 acagcgaaaa atccagcggt agcgtaaagt tggcgctgtt aagccagata tcgctctcga
    18181 tctctccttc atgccactga tcgtagctga tctctttgat ttccagtgtg acctggtggc
    18241 ttgccagaat ctgctgcatg atcccggcaa tcacccgatg ctcactgtga tcctgataaa
    18301 aggtcagggt gaggctttcc agaccagccg gtttttcgct ctttatggtg cgggcatgat
    18361 gccaacgggg gagcagtcca taagccggga accacagttg ctggtactgt tcctcagcga
    18421 aatagaccag attagttgga gaaagcacat agcttaccca gtccctgact tgctgattcg
    18481 ccccgcgatg ggtgcggcta tcgaacagta aatagtagca accttcctcc aggcggcttt
    18541 caatctcttt ttcctcgccc tgtggacctt ttagcatcag acctccggct ggctcgtcgg
    18601 caatttccgg cagaacccag acgttaactt cgtcgattaa tgcccggtaa ccgaagaagt
    18661 catcgaatgc ctgaattttc agttgattgg tgctgttgcg gatcaccgca tacggaccgg
    18721 tgccgatggg atggctggca aagttactga gggtttccca ttcgcgcggc aggatcatcg
    18781 ccggaacttg tcccagcagt aacggcaacc agcgatccgg ctgcgtgagg tggatatcca
    18841 gcgtccaggg cgtcggcgac acaatgtcag caatatgcga atagagcggc agcgtattga
    18901 ttcgttttaa agaggcgatc acgtcgtcca tttccagttc acgaccatga tgaaaatgga
    18961 ctcctggacg caaaaagaaa cgccagtgaa gcggtgatat ttgctgccag tggtgggcga
    19021 tgtctgcttc cagttcccca ttttcctcat ttatgcgcgt tagcgaactg aagatttgcc
    19081 gggcgatatg ggtttcggaa cggcgcaatg cgctgccagg tagcaggtta cgcaacggac
    19141 gatagtagag cacgcgcagg atgtgccgcc cctggcggaa gctgcggccc agatgagaaa
    19201 ccagcatttg cctcacagtc gctttgtcgc caactaactg cactagttgg tcgatacggt
    19261 cctgctccag caggtcttcc gcccgctgtt gctgaagcgc cagcccggta tagaggaatg
    19321 tcagacgcga gcgtttacca cgcccgactt ccgcttccca cgtcagccag ccgcgatcct
    19381 gcatggtgtt gagcagggtg cgcatatgac gacgcgagca gctcaataac gctgccagtt
    19441 cgttgagcgt tgtgtcctgc gatttaccct cgcagcattg ccacaggcgg atgaactgtt
    19501 gttgcagacg agcagatggc ataaaagggg aactcctgcg caaaagacag caattttatt
    19561 ttccctatat taagtcaata attcctaacg atgaagcaag gaggtgcccc atgcgtcagt
    19621 tttatcagca ctattttacc gcgacagcga agttgtgctg gttgcgttgg ttaagcgtcc
    19681 cacaacgatt aaccatgctt gaagaactga tgcagtggga ggggagtcat tctgactact
    19741 gatttgcgga catcatgtgt gactgagtat tggtgttaat cgccacgcca gcagtgatta
    19801 tctgctggct tttttctttt tggcgattga gtaccggacg gtgtacaaac taacgcataa
    19861 acgcgggttg cttcgcttca taagccgcaa tggcgtcgtc gtgctgcaag gtaagcccaa
    19921 tgctgtccag accgttcatc aggcagtggc ggcggaaggc atcaatggta aagcgatagg
    19981 ttttctcacc cgctttcacc tcttgcgctt ccaggtctac atcaaaatga atccccggat
    20041 tggctttcac cagcgcaaac agctcatcca cttctgcgtc gcttaatttc accggcagca
    20101 gctggttgtt aaagctattg ccatagaaga tgtcagcaaa actcggcgca atcaccacct
    20161 taaaaccgta atcggtcaat gcccagggcg cgtgctcacg cgaggagcca cagccgaagt
    20221 tttctcgtgc cagcaaaatg gaagcgccct gatactgcgg gaagttcagc acgaagtccg
    20281 ggtttggctg ttggcctttt tcatccagaa aacgccagtc gttaaacaga tgcgcgccaa
    20341 aaccagtacg ggtcactttc tgcaaaaact gtttcgggat gattgcatcg gtatcgacat
    20401 tggcggcatc cagcggaacc accaggcctg tgtgtttgat aaatttctct gccatggtgt
    20461 gctccttatt taatgttgcg aatgtcggcg aaatgtccgg tcacagccgc ggctgcagcc
    20521 attgccgggc tgaccagatg cgtgcgcccg ccgcgcccct gacggccttc aaagttacgg
    20581 ttgctggtgg aggcacaacg ttcgcccgga ttcagacggt cgttgttcat cgccagacac
    20641 attgagcagc caggcaagcg ccattcaaaa ccggcttcaa taaagatttt atccagacct
    20701 tccgcttccg cctgggcttt taccgggcca gagccgggaa ccaccagtgc ctgcacgcct
    20761 ggcgcgactt ttcgcccttt ggcgatctcc gctgccgcgc gtaaatcttc aatgcgcgag
    20821 ttggtacagg aaccgataaa cactttgtcg atagccactt cggtcagcgg aatacccggt
    20881 ttcagcccca tataggccag cgctttttct gccgacgcac gttcaaccgg atcggcaaac
    20941 gaagccggat cgggaatatt gtcgttcacg gaaatcacct ggcctggatt ggtgccccag
    21001 gtgacctgcg gcgaaatctc ttctgcttgc agagtgacaa cggtatcgaa agttgcgcct
    21061 tcgtcggttt gcagggtttt ccagtaggca acggcgtcgt cgaaatcttt gcctttcggc
    21121 gcatgcagac gacctttgac atagttaaag gtggtttcgt ccggtgcaac cagaccggct
    21181 ttcgcgccca tttcgattgc catattgcac agggtcatcc gaccttccat gcttaaatca
    21241 cggattgctt cgccgcaaaa ctccaccaca tgcccggtgc cgcctgcgct gccagtttta
    21301 ccgataattg ccaacacgat atcttttgcg gtaatgcccg gcgcggcttt gccctggact
    21361 tcaattttca tggtcttcgc acggccctgt ttcagggttt gcgttgccag tacgtgttca
    21421 acttcggaag tgccgatacc aaaggccagt gcgccaaacg cgccgtgagt ggcggtatgc
    21481 gagtcgccgc agacaatggt catccccggc aaggtcacgc cctgttccgg ccccattacg
    21541 tggacgatcc cttgataggg gtgattcagg tcatacagtt cgacgccaaa ttctttgcag
    21601 tttttgatca gttcctgcat ctggatacgc gccatttcac cgcaggcatt aatgtctttg
    21661 gtctgggtag agacgttgtg atccatggta gcgaaggttt tgcccggctg acgtaccggg
    21721 cgaccgtggg cgcgcagacc atcgaacgcc tgcggtgagg tcacttcatg caccaggtgg
    21781 cggtcgatat ataacagcgg ggtttcgttt tcggcttcgt acactacgtg agcgtcaaac
    21841 aatttttcgt ataacgtctt agccatgatt acaccccttc tgccacatag cgggcaatga
    21901 tatcgcccat ttcatcggta ctaacggcgg cagcgccacg ggctaaatcc ccggtgcgaa
    21961 tgccttcttc taatgcgcgg ttaatggcgc gttcaatggc ggaagccgca tcatcggcat
    22021 ccaggctgta acgcagcagc agtgccagcg acaggatttg tgcaatcggg ttggcgatgt
    22081 ttttgcctgc gatatctggt gccgagccgc ctgccggttc atacagacca aaaccttgct
    22141 cgttcaggct ggcggaaggc aacatgccca tcgagccagt gatcattgcg cactcgtcag
    22201 acagaatgtc gccaaacagg ttggagcaca gcaggacgtc aaactgtgat ggatctttaa
    22261 tcagctgcat ggtggcgttg tcgatgtaca tatgcgccag ttcgacatcc gggtattccg
    22321 tggcgatctc gttgacgatt tcacgccata aaatagagga ttgcagcacg ttagctttgt
    22381 cgattgaggt gactttgtgg cgacgcttgc gggcagattc aaacgcgatg cgggcgatac
    22441 gttcaatctc aaaacggtga tacacctcgg tatcaaacgc tttttcatat tgtccgctac
    22501 cttcgcggcc ttttggctga ccgaaataga tgccgccggt cagttcacgc acgcacagga
    22561 tgtcgaagcc gtttgcggca atgtctgcac gcagcggaca gaatgcttcc agcccctgat
    22621 acagttttgc cgggcgcagg ttgctgaata atttgaagtg cttacgcaga ggcagcagcg
    22681 cgccgcgttc tggttgctgg tctggtggta aatgttccca cttcggaccg cctaccgagc
    22741 caaacagcac ggcatcggct tgctcacaac cttcaaccgt ctcaggcggc agcggctgcc
    22801 cgtggttatc aatggctgcg ccgcctacat cataatggct ggtggtgatg cgcatcgcaa
    22861 agcggttgcg cacggcatcc agcactttca gcgcctgggt catcacttcc ggcccgatcc
    22921 catctccggg caaaacggca atatggtaat tcttcgacat cacacggttt ccttgttgtt
    22981 ttcgttgtgt tgagctttgc gttgcaactc tttttcgact tctgcggcac gccagatatt
    23041 gttcagtacg tgcaccatgg ctttggcgga ggactcgaca atatcggtgg ccaggccgac
    23101 gccgtggaag cggcgaccgt tgtagttggc gacaatatcc acctgaccca gcgcatcttt
    23161 accgtgacct ttggcggtca ggctgtattt caccagttcg acgttatagt cagtgatgcg
    23221 gttaatcgcc tgatagacgg catcgaccgg accgttaccg ttggcggctt ctgctttgac
    23281 ttcttcgcca caggccagtt tgacggcggc ggtggcgata tcgttagagc cagactgcac
    23341 gctgaagtaa tccagacgga aatgctccgg ctcttcttgc tgcttaccga tgaaggccag
    23401 cgcctccaga tcgtaatcaa acacctgacc ttttttgtcc gccagcttca ggaaggcgtc
    23461 gtacaaattg tctaaattat attcactttc tttatacccc atctcatcca tgcgatgttt
    23521 caccgccgca cgccccgaac gagaggtcag attcagctgg atttggttca gaccaataga
    23581 ttctggtgtc atgatttcgt agttttcgcg gtttttcagc acaccatcct ggtggatacc
    23641 ggaggagtgt gcgaatgcgc cgctgccaac aatggctttg tttgccggga tcggcatatt
    23701 acaaatctgg ctaactaact ggctggtgcg ccatatctcc tggtgattaa tggcggtgtg
    23761 gacgttgaga atatccttac gaactttaat cgccatgatg acttcttcca gggaacagtt
    23821 accggcacgc tcgccgatcc cgttcattgc accttccacc tggcgtgcac cggcatgtac
    23881 cgccgccagt gagttgccga ccgccaggcc caaatcgtcg tgggtatgta cggaaataat
    23941 ggctttgtcg atgttaggca cgcgttcata caggccgctg atgattccgg cgaactcaaa
    24001 cggcatggtg tagcccacgg tgtccggaat gttgatggtg gtggcaccgg cattaatcgc
    24061 cgcttcgacc actcgcgcca gatcggcaat gggtgtacgc ccggcatctt cgcaagaaaa
    24121 ttcaacatca tcggtgtaat tacgggcgcg tttcaccata tagatagcgc gttcgatcac
    24181 ctcgtccagc gtgctgcgca gcttggtggc gatgtgcatt ggcgaagtgg caataaaggt
    24241 atgaatacgg aaggcttcgg cgactttcag ggattcggcc gccacgtcga tatctttctc
    24301 cacgcagcga gctaacgcgc atacacggct gtttttaacc tggcgagcga tggtttgcac
    24361 tgattcaaaa tcacccggcg aagagacggg gaacccgact tccatcacgt caacacccat
    24421 acgctcaagg gccagcgcaa tttgcagttt ttctttcaca ctcaagcttg cctgtaacgc
    24481 ctgttcaccg tcgcgcaatg tggtatcgaa aataatgact tgctggctca tggtttgggt
    24541 ccttgtctct tttagagcgc ctcgcttcgg gcataaaaaa acccgcgcaa tggcgcgggt
    24601 tttttgtttg actgcgtgct ggcttaatgc tggatgccgc tcactcgtct accgcgcaaa
    24661 gaagatgcgt ttagtagtag tagaccgata aagcgaacga tgtgagtcat taaatcagct
    24721 ccagatgaat gcgatatgct tttagagtta ctggatacaa aaacggatgt caaccctgac
    24781 gcaataaaaa cgtcccgcca gcatgagttc tgcatccgta agattagcta attgtgctgc
    24841 tgtggttaaa acaagcgata ttaatttctg cttaactacc gatgcttttc atcggttgac
    24901 atatttcaga ataaattttt gcatctaatc cacgaggaaa aaggggacaa aatgcacgcg
    24961 tt
//
LOCUS       DAZUCE010000055        24905 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZUCE010000055 DAZUCE010000000
VERSION     DAZUCE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.055"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..603
                     /locus_tag="ACHY03_001885"
     CDS             <1..603
                     /locus_tag="ACHY03_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFS3905121.1"
                     /translation="TLRIFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRA
                     DLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQQAQAQV
                     TRTLGQLTIISERYPELKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNANAH"
     gene            617..1288
                     /locus_tag="ACHY03_001886"
                     /pseudo
     CDS             617..1288
                     /locus_tag="ACHY03_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            1525..2673
                     /locus_tag="ACHY03_001887"
     CDS             1525..2673
                     /locus_tag="ACHY03_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS3905122.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQTALTQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEQMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFASRPGRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSIVCFSLLLATTCKQRLAVVFNLCRVFPMMLAPKNKNAKVKKQKKTPQVR
                     ESGRNSLFVVMLIIIVGWCLVFASIKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSGRW"
     gene            2670..3584
                     /locus_tag="ACHY03_001888"
     CDS             2670..3584
                     /locus_tag="ACHY03_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFS3905123.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKERASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(3644..4942)
                     /gene="eno"
                     /locus_tag="ACHY03_001889"
     CDS             complement(3644..4942)
                     /gene="eno"
                     /locus_tag="ACHY03_001889"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFS3905124.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(5030..6667)
                     /gene="pyrG"
                     /locus_tag="ACHY03_001890"
     CDS             complement(5030..6667)
                     /gene="pyrG"
                     /locus_tag="ACHY03_001890"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFS3905125.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6895..7686)
                     /gene="mazG"
                     /locus_tag="ACHY03_001891"
     CDS             complement(6895..7686)
                     /gene="mazG"
                     /locus_tag="ACHY03_001891"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFS3905126.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFSSIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     GSAGNSREVLARWEEIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7757..8092)
                     /gene="mazF"
                     /locus_tag="ACHY03_001892"
     CDS             complement(7757..8092)
                     /gene="mazF"
                     /locus_tag="ACHY03_001892"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFS3905127.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(8092..8340)
                     /gene="mazE"
                     /locus_tag="ACHY03_001893"
     CDS             complement(8092..8340)
                     /gene="mazE"
                     /locus_tag="ACHY03_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFS3905128.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(8418..10652)
                     /gene="relA"
                     /locus_tag="ACHY03_001894"
     CDS             complement(8418..10652)
                     /gene="relA"
                     /locus_tag="ACHY03_001894"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFS3905129.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10700..12001)
                     /gene="rlmD"
                     /locus_tag="ACHY03_001895"
     CDS             complement(10700..12001)
                     /gene="rlmD"
                     /locus_tag="ACHY03_001895"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFS3905130.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            12058..14814
                     /gene="barA"
                     /locus_tag="ACHY03_001896"
     CDS             12058..14814
                     /gene="barA"
                     /locus_tag="ACHY03_001896"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFS3905131.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(15047..16387)
                     /gene="gudD"
                     /locus_tag="ACHY03_001897"
     CDS             complement(15047..16387)
                     /gene="gudD"
                     /locus_tag="ACHY03_001897"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFS3905132.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDADGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLLQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(16408..17748)
                     /gene="gudX"
                     /locus_tag="ACHY03_001898"
     CDS             complement(16408..17748)
                     /gene="gudX"
                     /locus_tag="ACHY03_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFS3905133.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKNFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(17750..19102)
                     /gene="gudP"
                     /locus_tag="ACHY03_001899"
     CDS             complement(17750..19102)
                     /gene="gudP"
                     /locus_tag="ACHY03_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFS3905134.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(19537..19986)
                     /gene="yqcA"
                     /locus_tag="ACHY03_001900"
     CDS             complement(19537..19986)
                     /gene="yqcA"
                     /locus_tag="ACHY03_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFS3905135.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(20004..20786)
                     /gene="truC"
                     /locus_tag="ACHY03_001901"
     CDS             complement(20004..20786)
                     /gene="truC"
                     /locus_tag="ACHY03_001901"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFS3905136.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(20786..21115)
                     /gene="yqcC"
                     /locus_tag="ACHY03_001902"
     CDS             complement(20786..21115)
                     /gene="yqcC"
                     /locus_tag="ACHY03_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFS3905137.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21737..22282)
                     /gene="syd"
                     /locus_tag="ACHY03_001903"
     CDS             complement(21737..22282)
                     /gene="syd"
                     /locus_tag="ACHY03_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFS3905138.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            22350..23198
                     /gene="queF"
                     /locus_tag="ACHY03_001904"
     CDS             22350..23198
                     /gene="queF"
                     /locus_tag="ACHY03_001904"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFS3905139.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGNVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            23310..24674
                     /gene="ppnN"
                     /locus_tag="ACHY03_001905"
     CDS             23310..24674
                     /gene="ppnN"
                     /locus_tag="ACHY03_001905"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFS3905140.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         6145 a         6517 c         5953 g         6290 t
ORIGIN      
        1 acgctcagaa tattcatcgt aattatattt attttcaatt tatcaggctg tggatataac
       61 gatattcaaa cctatgatga gcaggttaac gcctcctggt ctgaggtatt gaatcaatat
      121 caacgtcgcg ccgatcttat tcctaatctt gtcgccagca ttaaaggtta ttccagccat
      181 gaacgagatg tactggaagc cgtaacattg gctcgtagcc aggctaatcg cgccagtagc
      241 gatcttcagc aaactccggg agatgaacaa aaattacaag cctggcaaca ggctcaagcg
      301 caggtgactc gcactctcgg tcagttaacg ataattagtg agcgttaccc tgagttaaaa
      361 tctcaggaac tctatcaaaa tttaatggta cagcttgaag gaagtgaaaa tcggattgcc
      421 gttgcgcgag gaagatatat taaagccatt gaacaatata atgtcactat ccgcaaattc
      481 cccgctgtat taaccgcaaa ggtcatggat tacacaccaa agaaaaatta tttgccggat
      541 gatgtcgccg cagtaagtaa agccccaaca atagatttta gccagaatgc taatgctcat
      601 taagcgggga agttctatgc gaaattttat cttccttatg gctttcttct gttcatctgt
      661 gtttgccaca caaattccag tacctgaatc acccaagtat gtgaatgact taaccggcac
      721 attaacaaac agcgaagtaa acaccctgac caaccagatt aaagccctga cgcaaaaaaa
      781 ctacgcacag ttggtcgtgt tagtggttga aacaacaggc gatgaaacca tcgagcagta
      841 cgcgacgcgg gtattcgata gctggaaacc gggtgataaa gaccgcgatg atggcgttct
      901 actgttagtc gcctggcagg atcataccgt gcgtattgaa atcggttacg gactggaagg
      961 tattattacg gatgcgcagt ccgggaaaat tattcgcaac agtattattc ctgcatttaa
     1021 aaaaggcgac ctggctggtg gtttgcaaaa gggaatcagc gatattgaaa gtcgtctgac
     1081 ggggaataat ttagcaacga taacaccgac cgatcaccct ttgcccttta gcggttggtg
     1141 ggcattgtta gtctgggcga tagtactgac gtttatttct gccagaggat atatcaaaac
     1201 gctgggagta atttgcttcg cagctattgt actggccttt gttttaccta tagcaggctt
     1261 cagcggaagc tggggagtac tcgcaacatt actctgcttc gccacaccgt tcctggctgt
     1321 cgccatcgct tttaccccct tcggcaaaaa agttcgggat ttgatgcgtg atgccaacca
     1381 gccatcacgc catacaagaa gcaattcttc ctcccactat tcatcatcgt catcctcttc
     1441 attcgacaat gacaatttta gcggtggtgg cggttcatct ggtggcggtg gcgcttccgg
     1501 gcgctggtaa caaaaggaat tgatatgcgt atttttctct tacttctgat gctatgcggt
     1561 tttcaggtat tcgctgaacg attgccttca gtgactgact taaccgggac attaacgaca
     1621 gaagaacaaa ctgctttaac tcaacaatta cagactctcg agcagcaaaa ccacttccag
     1681 gtggccgtgt tggttacgcc gaccacaggg ggcaaaaaca ttaaacttgc agcctcgcag
     1741 agatacgggg tatatcactg gaaacccgtt ggagagaagc gatatggcga aggtatctta
     1801 attctcgtcg tatggccgga agggctggcg tcaatgaaga ttggacatgg actggaacag
     1861 atgttgccgc cggaacaagc tgcgcaaatt gttcgttacc atatgcagcc agaattcgaa
     1921 aaaaacaatc tctttgctgg attaacgggc ggaattgaaa gcatcgcaca gtttacccat
     1981 atcaaagcga accttagccc tttggatgct ttagccaatc atctgtttgc taatccgcaa
     2041 cgttcgctgc catgtcttgc ctggacactg ctgatgatcg ttgccatcgt tgtcctctgg
     2101 cgatttgcca gccgcccagg gcgtggcata tggatgatat cgatgattac ccctatggtc
     2161 tggtttttct cctttcagga cgatgtcatc attcggcggg tgtcgatcgt ttgttttagt
     2221 ttattgttag caacaacctg caagcagcgt ctcgcggtag tttttaattt gtgccgcgta
     2281 tttcctatga tgctggcccc aaaaaataaa aacgctaaag tgaaaaaaca aaagaagact
     2341 ccgcaggttc gtgaatcagg acgaaattca ttgtttgtcg tgatgctgat aattattgtg
     2401 ggatggtgcc tggtgttcgc cagtattaaa gatattgcat tcatctcaac aattattggg
     2461 ttaattgatc attttatcgg tccaataacc attgctggca tcgttctgct gatcattgtg
     2521 gccaaattta ctggcaatct taagctggga tcaggagaaa aaagcaacag gaaaacgggg
     2581 aatcgtaatg ctcactctcg ctcttcgtcg agaaattctt tccgcggtgg tggcggttct
     2641 tcaggaggcg gcggttcatc cggccgttgg tgatcgtaat gcgcttaatc tttattctat
     2701 tcactttgtg gtgtcttcct gggcttgccc agcaaattgc tgtgccggaa ctgcgccaac
     2761 aggtgaccga tattactggt acgttaagca cttctgaaca acaatcactg acacagcaac
     2821 tgcaggatat tacacataaa acacgggcgc aggtcgctgt attagtagtg ccgtctaccg
     2881 gagacgattc cattgaacaa tatgctacaa gggtttttga tagctggaaa ctgggagata
     2941 agcaaagaaa cgatggtatt ttacttctgg ttgcctggga ggatcatgct gttcgtattg
     3001 aggttggcta cgggctggaa ggggtggtta ccgacctgca agcggcgaag attatcaggg
     3061 atatattgat ccctgccttc aaaagtgatg acctgatggg tggattaaca ctggcaagcg
     3121 aaaatatcgg cgcacttctg ttgaatggtg aattaccgga agacaggggg gattattaca
     3181 gtatcaatcc ccctattcca ttatcgcttg ctgtaattat attgctggcg gtactttcat
     3241 attttattgt ttttaccgat ccgtcgaatt taccctggat cactctcact ggcgccattt
     3301 acgggatggt attcctctat gttgccgagc caggaccatg gaccaattta atcgttgcct
     3361 gcggtatgtt aacgcctttt gcgatcgtac cactggttat tttttggctt atcgtcaata
     3421 aaaagctacg cgccaaatac aaaaaactca gtaaagaaag agcttcaaga aaaggttctt
     3481 cttcatcctc ttccggtggc ggctcaagtg gcggaggctc aggcggcggg tttagcggcg
     3541 gtggcggttc ttctggaggt ggcggcgcgt ccggtcgctg gtaactcaac cataaaaaac
     3601 gccagcccga aggctggcat ttttaaatca gataaagtca gacttatgcc tggcctttga
     3661 tctctttacg accgttgtac ggtgcttttt cgcccagagc ttcttcgata cgaatcagct
     3721 ggttgtattt agcaacgcgg tcagaacggc tcatagaacc agttttgatc tggcctgcag
     3781 cagtaccaac agccaggtca gcgatggtag cgtcttcagt ttcgccagaa cggtgagaga
     3841 taactgcagt gtagccagcg tctttcgcca tcttgattgc agccagagtt tcggtcagag
     3901 aaccgatctg gttgaatttg atcaggatgg agttagcgat acctttttcg ataccttctt
     3961 tcaggatctt ggtgttggtt acgaacaggt cgtcaccaac cagctggatt ttgtcgccca
     4021 gaactttggt ctggtatgcg aaaccatccc agtcagattc gtccagaccg tcttcgatgg
     4081 aaacgatcgg gtactgtttg gtcagttctt ccaggaagtg agtgaattct tcagaggtga
     4141 acgctttgtt gccttcgcca gccagaacgt atttaccgtc tttgtagaat tcagaagctg
     4201 cgcagtccat cgccagagtg atgtctttgc ccagttcata accagcagct ttaacagctt
     4261 cagcgataac agccagagct tcagcgttgg aacccaggtt cggcgcatag ccaccttcgt
     4321 caccaacagc agtgttcatg cctttcgctt tcagaacttt tgccaggtga tggaaaactt
     4381 cagaacccat gcggatggct tctttcacag ttttcgcgcc aaccggctga atcatgaatt
     4441 cctggatatc aacgttgttg tcagcgtgct caccaccgtt gatgatgttc atcatcggaa
     4501 ccggcatgga gtatttgccc ggagtgccgt tcagttcagc gatgtgctcg tacagcggca
     4561 tacctttagc agctgcagca gctttggcgt tagccagaga tacagccagg attgcgttcg
     4621 cgccgaattt ggatttgttt tcggtgccgt ccaggtcgat catgatcttg tcaatgccag
     4681 cctgatcttt agcatctttg ccaatcagcg cctgagcgat cgggccgttt accgcagcaa
     4741 cagctttggt tacgccttta cccaggaaac gggatttgtc gccatcgcgc agttccagag
     4801 cttcacggga accagtagaa gcacctgacg gagcagctgc cataccgacg aaaccaccct
     4861 ccagatgtac ttcggcttca acagtcgggt taccacggga gtcgatgatt tcacgaccga
     4921 tgatttttac gattttggac attaggtttt cctcaagtca ctagttaaac tgaaactcca
     4981 gacaaacaac gcgtacccag ggtacgcgtt gccgttctaa cttttttact tacttcgcct
     5041 gacgtttctg gaactcgctg gcggctttca caaagcctgc aaacagcggg tgaccatcac
     5101 gtggagtaga agtaaactcc ggatggaact ggcaagccac gaaccacggg tgattcggaa
     5161 cttcgatgat ctcgaccaac tgatcatccc cggaacggcc cgcaacgcgc agacctgcat
     5221 cttcaatctg tttcaacagc atgttgttga cttcgtaacg gtgacgatga cgctcaacaa
     5281 ttgtcggcgc attgtacagc tggcgaacca ggctatcgtc aaccaactgg cactgctgtg
     5341 cgccgagacg catggtaccg ccgagatcgc tcttctcgct acgaacttca acgttgccgt
     5401 tttcatcgcg ccactcggta atcagcgcca caaccgggta cttacagtct ggcacaaatt
     5461 ccgtagagtt ggcgttctcc atgttggcaa catggcgagc gtaatcaatt aacgccacct
     5521 gcatacccag gcaaatgccc agataaggaa tattgttctc acgcgcaaaa cgcgcggtcg
     5581 taatcatgcc ttctacgcca cgatagccga aaccgccagg tacgaggatt gcgtccagac
     5641 ctttaaggat ttcaacgccg cgcgtttcaa catcttgtga atcgatcagt ttgatgttga
     5701 cgctgacacg attcttcagc ccaccgtgtt tcagtgcttc aatcacggat ttgtaagcgt
     5761 ccggcagttc aatgtacttg ccgaccatac caatggtgac ttcacttacc ggattcgctt
     5821 cttcgaagat aacctgttcc cattcggaca gatttgcttc cgggcagttt aagctgaatc
     5881 gtttacaaat ataatcgtcc agcccctgag atttcaacag gcccggaatt ttatagatgg
     5941 aatcgacgtc tttcagagaa ataaccgctt tttccggaac attacagaac aatgcaatct
     6001 tcgcacgttc gttcgccgga acggcgcgat ctgaacgaca aatcaggatg tcaggctgga
     6061 taccgatgga gagcagctct tttacagagt gctgagtcgg tttggttttg acttcaccag
     6121 acgctgccat gtacggcacc agcgtcaggt gcataaacag agtgtgctca cggccaattt
     6181 caacagccat ctggcgaatc gcttcgagga acggcaggga ttcgatatca cctactgtac
     6241 cgccgatttc gaccagtact acgtcatgac cttcgccacc ttccagcacg cgctctttga
     6301 ttgcgttagt gatgtgcgga ataacctgta cggttgcgcc gaggtagtca ccgcggcgtt
     6361 ctttacgcag aacgtcagag tagatacgac ccgtggtgaa gttgttgcgg cggctcattt
     6421 tggtacggat gaaacgctcg tagtgcccca ggtccaggtc ggtttcagcg ccgtcttcag
     6481 taacgaacac ttccccgtgt tggattgggc tcatagtacc tggatcgacg ttgatgtacg
     6541 gatccagttt catgatggtc acattgaggc cacgggcttc aagaatggct gcgagggagg
     6601 ctgcggcaat gcctttaccc agagaggata cgaccccgcc ggtcacaaaa atatagttcg
     6661 ttgtcatgct gaacctgaga agttaggttg aaaagacgat ggaataacca ggacgggaaa
     6721 gcagtatacc cgaacatgac ctgtgccaca aactttcatt atccctcctc ttcgccagcg
     6781 cactattgaa atcaggagtg agaaaatagc ccctttgggg taaatgtttt tgacgcaaat
     6841 caagcgcttg tcatttaaaa aatgacacaa atggcgcttg accgcgtaat tcccttagag
     6901 atcaatttcc tgccgtttta cctgttgcca gacttcttcc attgtttcga ggtcaacacc
     6961 tgtcatttcc agtccacgcg cggcaacaat acgctccact tcgcgaaaac gacgctcgaa
     7021 tttttcgttc gctttttgca atgcgatttc cgcttttgtc cctaaatgac gagccagatt
     7081 gaccgtggca aacagcaggt cccccatttc ctcctccagt ttagcctggt cgacaacagc
     7141 ctgccgcgct tcgtacatca cctcgtcgat ctcttcgtag actttatcga ctaccggacc
     7201 aagcgtcgtc caatcgaagc caacgttggc gcaacgtttc tggatttttt gcgcacgcat
     7261 taaggcgggt aaactacgag gaatatcgtc cagcgccgaa tgctgcgctt tctgcgcgcg
     7321 ctcttcggtt ttgatctcct cccaacgggc gagaacttcg cggctgtttc cggcagaacc
     7381 gtcagcaaaa acatgtggat gacggcgttc taatttatcg ctaatcgcgg cgcaaatatc
     7441 attaaaatca aagcgccctt cttcctgggc catttgtgcg taaaacacca cctggaacag
     7501 cagatcgccg agttcaccgc gcagatcgtc aaagtcttcg cgggcaatag cgtccagcac
     7561 ctcgtaggtt tcttcaaggg tgtaaggcgc aatagaagaa aatgtctgct ctttatccca
     7621 cgggcagccg ttttccgggt cgcgcaggcg ctgcataata gtgagcaaac ggtcgatttg
     7681 attcattaga atgtcctgaa aattgcgggt ctgtcaggtg gaaacctgtg accagaatag
     7741 aagtgagtta gtaacactac ccaatcagta cgttaatttt ggctttaatg agttgtaatt
     7801 cctctggggc aaccgttcct ttcttcgttg ctcctcttgc ccgccaggcg atacttttta
     7861 cctgatcagc taacgctacg ccatcacgtt cctgaccgga taaaacaact tcgaacggat
     7921 atccttttga ttgcgttgta caaggaacac acagacacat acctgttttg ttgttgtaca
     7981 tgaacggact caggacaaca gccggacgat gtccggcttg ctcgctacct tttgtcgggt
     8041 caaaatcaac ccaaatcaga tcgcccatat cgggtacgta tcggcttacc attaccagac
     8101 ttccttatct ttcggctctc cccagtcgat attctcgtgg aggttttccg gcgtgatgtc
     8161 gttgaccagt tcagcaagcg taaatacggg ctctttacgc actggctcaa taattaattt
     8221 gccatccacc aggtcaatct tcacttcatc atcaatattc agattgagcg cctgcattaa
     8281 cgtagccggg atccgcaccg ccggtgaatt tccccaacgc tttacgctac tgtggatcat
     8341 aaccctttcc tcaaaccgct atcatatgta gatacagtat atatcaatcc acattgtaga
     8401 tacgagcaaa tttctgccta actcccgtgc aaccgacgcg cgtcgataac atccggcacc
     8461 tggttgagtt tacccagcac gcgacccagc acctgcaggt tgtaaatctc aatggtcatg
     8521 tcgatggtcg ccagttgctg tttggtgtcg ctacggctgg caacgccaag tacgttcacc
     8581 ttctcgttgg cgagaatggt cgtgatatca cgtaacaacc cactacgatc attagccacc
     8641 acgcggacca ccagcgaata tccggcggag tagctctcgc cccataccgc gtcaacaatg
     8701 cgttctggcg catgggagcg cagttccgcc agttgttcgc aatcggcgcg gtgtactgaa
     8761 ataccgcgtc cctgggtaat gaagccgaca atctcatctc caggaatcgg ctggcagcag
     8821 cgcgcgatgt ggtgcatcag gttaccaaca ccttcaacca ccacacgacc gttatcttta
     8881 ctgcggtttt gcggtgtgta gcttttttgc tgaagctgtt tcagcgcggc ggcgtcctgc
     8941 tcttcggcac tcggcttatt aaattgcgat tgcaggaagt tcaccatctg attgagacgg
     9001 atatccccgc caccaatcgc cgccagcaac tcgtcgacat cattgaagtt gtaacgcggc
     9061 agcagatgtt tttctgcttc tttcaggctg atccccagat gttccagctc gtcgtcaagg
     9121 atttgccgcc cagccagaat gtttttgtca cggtcctgtt tacggaacca ggcgtgaatt
     9181 ttcgaacgcc cacggctggt tgtgacgtaa ccgaggtttg ggtttaacca gtcacggctg
     9241 gggttcggct gtttctgggt gataatttca atctggtcgc ccatttgcag ctggtaggtg
     9301 aacggcacaa tgcgcccgcc aatttttgcc ccgatgcagc ggtgtccgac atcactgtgg
     9361 atgtggtaag cgaagtccag cggcgttgat cccgcaggca aatcaacgac atcacctttc
     9421 ggcgtaaaga catacacccg gtcgtcaaac acctgactac gtacttcgtc gagcatttcg
     9481 ccggaatcag ccatctcttc ctgccacgca atcagtttac gcagccaggc aatccggtct
     9541 tcatgtcccg aacgtgcgcc gccagctgcc gcgccctctt tatatttcca gtgcgcagca
     9601 acacccaact ctgcatcttc atgcatctgt ttggtgcgga tttggatctc aacggttttg
     9661 ccacctggcc ccagaaccac ggtatgaata gactgataac cgtttggttt cgggttagcg
     9721 acgtaatcgt caaactcatc cggcaggtgg cgatagtgag tgtgcactat ccccagtgcg
     9781 gcataacaat cctgtaaacg ctcggcgaca atacgtaccg cacgcacatc aaacagctca
     9841 tcgaaggcga ggttcttttt ctgcattttg cgccagatgc tgtagatgtg tttcggacga
     9901 ccatacactt cagctttaac gccttcagct ttcatctcag cgcgcagatg accgacaaac
     9961 tcttcgatat agtgttcgcg gtcgagacgg cgttcatgca acagttttgc gatgcgtttg
    10021 tattcggtcg ggtggagata acggaagcag taatcttcca gctcccattt cagttgccca
    10081 atcccaagac ggtttgccaa cggcgcgtag atattggtgc actcttttgc cgccagtacg
    10141 cgttcatctt ccggcgcatc ttttacttca cgcagatgag caatacgctc cgccagtttg
    10201 atgaccacgc agcgaaaatc atcgaccatc gccaataaca tccggcgaac gttatcgacc
    10261 tgttcggagg aaacagaatc agtgtgcgtc gctttcagct ggcggatcgc cgccatatca
    10321 cgcacgccgt gaataaggtt aacgaccgac ttaccgacgc tctcacgcag cacatcttcg
    10381 ctgactacgt tggcatccgc cagagggaaa agcagcgccg cccgcagcgt gtcaatgtcc
    10441 atacttaatg tcgagaggat ctccaccatc tcaacaccac gccacaataa cagactggca
    10501 tccggatgcc cctgcgtctg ttgcagacaa tacgcccagg tttcggctaa gcactcacac
    10561 gacttctggc tggtaatacc cagacttgcg atccattttt ccggatcaaa ttcaccagcc
    10621 ttattgatat gtgcacttct taccgcaacc atcgtcctct cctttaggga ccagacctgc
    10681 cgaaatcggc aaatcgcaac tatttaacgc gcgagaaaag taccatcgat tccagatgtc
    10741 ccgtgtgtgg gaacatatcc agcatcgcca gtcgcgcaat ggtatatcct gcttttaata
    10801 acgcttcgct atcccgagcc agcgttgcag ggttacagga tacataaact atacgaatag
    10861 gttccagttt tataatttgc tgcataacac ctgcggcacc tgctcgcgcg gggtccagca
    10921 acactttatc gaagccgttt ttcgcccacg gctgctttgt gacatcttct tcaagatttt
    10981 catgataaaa cgtcacattc tgtaagccat taagacgcgc attctgctgg cctttttcca
    11041 ccagcgcagg aacaccttct acacccacca cactggctgc ttgtgtcgcc aatggcagtg
    11101 taaagttgcc cataccgcag aacagatcca gtacgcgatc ttcaggttgc acatccagcc
    11161 attccaacgc acgcgctacc attttttggt tcacacccgc attgacctga ataaaatcgc
    11221 gcgggctaaa agttaagcgc aacccatttg agtcatacca gggcatctca ccagagacgg
    11281 tttcgagtat ctcactatcg ggggcgagat acagatccag gccttcagaa tgcgaaaagc
    11341 gttccagttt ttcgcgatct gccgaactta gcggtgcggt atggcgcaaa atcatcagcg
    11401 tgccgctggt tgcctgtact agttcaacat gaccaagatg gcgcatagct tgtaagctgc
    11461 ccaggcatgc cctgactttg ggcagcaatg cttcaagttg gggcactaaa atggggcatt
    11521 gtttaacgtc gacaatgtca ctggagcccg ctttgcgaaa ccccatctga agttgctgtg
    11581 ttttcggtaa gtagttcaga cttaaacgcg cgcgacggcg atagccccag ggaacatcgg
    11641 cgatcacttc agagacttcg tgtttcatta atcgggcgag tgccgcactt ttgcttcgct
    11701 gctgtaaatc cacgctggcg tgttgttgct gacagccgcc gcatacgcca aaatgaggac
    11761 agcgtggcgt ttcgcgttcc gggctatcgc ttaaccggcg tacgacttta gcgcgggcat
    11821 actgtttttt atcttcagta acagtaactt ccgcgttttc ctgcggcaat aatccgggga
    11881 taaatagcgt tttgccgtta tgtcgcgcca cgccctgacc aaaagagtcg aggtcgttga
    11941 ctgaaacggt tatgatctga cgcgtcgtcg tgcgtcgttt tgcagagtag aattgcgcca
    12001 ttggcgagac tttctcaatt taacagtgtg accttaattg tcccataacg gaactccatg
    12061 accaactaca gcctgcgcgc acgcatgatg attctgatcc tggcaccgac cgtccttatt
    12121 ggcttattgc tgagtatctt tttcgtcgtg catcgctata acgacttgca gcgtcaactg
    12181 gaagatgccg gtgccagcat tattgagccg cttgcagttt ctactgaata tggcatgagc
    12241 ctgcaaaatc gcgaatctat cggtcagtta ataagcgtac tgcatcgtcg ccattccgat
    12301 attgttcgcg cgatttcggt ttatgatgaa aataaccgac tctttgtcac ctccaatttt
    12361 catcttgatc cctcatcaat gcaactcggc agcaacgtgc cgtttcctcg ccagctcact
    12421 gtcactcgtg acggcgatat tatgatcctc cgcacgccga ttatttctga gagttactcc
    12481 cccgacgaat cgcccagtag cgatgccaaa aatagtcaaa atatgttggg atatattgcg
    12541 cttgagctgg atcttaaatc ggttcgcttg cagcaatata aagagatctt tatttccagc
    12601 gtgatgatgc tgttttgtat cggtattgcg cttatttttg gctggcgctt aatgcgcgat
    12661 gtaaccggtc cgattcgcaa catggtgaat accgtcgacc gcatccgtcg cgggcaactc
    12721 gacagccggg tggaaggatt tatgctcggc gagctggata tgctgaaaaa cggtatcaac
    12781 tcgatggcaa tgtcgctggc tgcttatcac gaagagatgc agcacaatat cgaccaggcg
    12841 acgtccgatc tgcgtgaaac gctggagcag atggaaattc agaacgttga gttggatctg
    12901 gcgaaaaagc gcgcccagga agcggcacgt attaaatccg agtttctggc aaatatgtca
    12961 cacgagctgc gtacaccact gaatggtgtt attggcttta cccgcctgac gctgaaaaca
    13021 gaattaacac caacgcagcg cgatcacctg aatacgattg aacgttcggc aaataatttg
    13081 ctggcaatta ttaatgatgt tctcgacttc tcgaaactgg aagcaggtaa gctgattctg
    13141 gaaagtattc cattcccact acgcagcacg ctggatgaag tcgttaccct gctggctcat
    13201 tcttctcacg ataaagggct ggaactgacg ctcaatatta agagcgacgt gcctgataac
    13261 gtgatcggcg acccactgcg attacagcaa atcatcacta acctggtggg gaatgcaatt
    13321 aaattcaccg agaatggcaa cattgatatt ctggtagaaa aacgtgcgct gagtaatacc
    13381 aaagtgcaga ttgaagtgca gattcgggat accggcattg gtattcctga acgtgatcaa
    13441 tcgcgcttat tccaggcctt ccgacaggct gatgccagta tttcccgccg tcatggtggc
    13501 accgggctgg ggctggtgat tacacaaaaa ctggttaatg aaatgggcgg cgatatttcg
    13561 ttccatagcc agccgaatcg cggttcaact ttctggttcc acattaatct cgatctgaac
    13621 ccgaacatta ttattgaagg gccatccacc cagtgcctcg ccggtaaacg cctggcctat
    13681 gtcgaaccaa actccgcagc agcgcaatgc acgctggata ttttaagtga aacgccgctg
    13741 gaagtggttt atagcccaac gttctccgcg ctgcctcccg ctcattacga catgatgttg
    13801 ttaggcatcg cggtgacctt ccgcgagccg ttaacaatgc aacatgagcg attagcgaaa
    13861 gcggtatcga tgaccgattt cctgatgctg gcacttcctt gccatgcaca agtcaatgct
    13921 gaaaaactca agcaagatgg tatcggcgcg tgtctgctga aaccattaac acctacgcgc
    13981 ctgttgcctg ccctgacgga attttgtcat cacaaacaaa acacgctttt gcctgtaacc
    14041 gatgaaagta agctggcaat gacagtcatg gcggttgatg acaaccccgc taacctgaaa
    14101 cttatcggcg cattgctgga agatatggtg caacatgtgg aactttgcga tagcgggcat
    14161 caggcggttg aacgggcgaa acagatgccg ttcgatttga tcttaatgga tattcaaatg
    14221 cctgacatgg atggcattcg ggcctgcgag ctcatccacc agctccccca tcagcaacaa
    14281 acgccggtta tcgcggtaac ggcgcatgca atggccgggc aaaaagagaa gctgcttggc
    14341 gcaggaatga gcgattatct ggcgaaaccg attgaagaag agcgattgca taatttgttg
    14401 ttgcgctaca agcctggcag cggtatttcg tctcgcgtcg tgacgcccga agtcaacgaa
    14461 attgtggtga acccgaatgc gaccctcgac tggcaactgg cactacgcca ggcagcagga
    14521 aaaaccgatt tagcgcgcga tatgctgcaa atgttactcg atttcctgcc tgaagttcgc
    14581 aacaaagttg aggaacagct ggttggagaa aatccggaag gcctggttga tttgattcat
    14641 aaactgcatg gcagttgcgg ctatagcggt gtgccgcgta tgaagaatct ctgccaactg
    14701 atcgaacaac agctacgtag cggtactaaa gaagaagatt tggagccgga gctgctggaa
    14761 ctgttggacg agatggataa tgtcgcgcgt gaagccagca aaattctcgg gtaacggacg
    14821 tcttgtcgag tagcaatcgg ctttttatga cgctggtttc agattggtga caaagtgcct
    14881 gtgtttatgc cggatgcggc gttaacgcct tatccggcct acaaaaccaa ttaaattcaa
    14941 taaattaaaa agcatgtagg cctgataagc gtagcgcatc aggcaatttt gtgtttggca
    15001 tcagtctcaa accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc
    15061 acgggcgctt gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt
    15121 cacgcgcgcc aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat
    15181 cgatttctac acccagcccc ggtttttctg gcacctgtag cagcccgcct ttgatctcaa
    15241 acggttcttt ggtcaggcgc tgattgcctt cctgccaaat ccagtgcgta tcaatagcag
    15301 taattttacc cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt
    15361 ggttgttaga gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta
    15421 ccgaaccttg cattgtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg
    15481 agagcgtatg gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc
    15541 cagtcgcgcg acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac
    15601 acggatcttc tgcataagcc agcgaacctt tcaggtattt accaatttta atcgcttcgt
    15661 tcagcgacca ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca
    15721 gtgccacaat agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat
    15781 cgttgaaacc atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca
    15841 tcgcttcttc atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg
    15901 gcagcggtgt ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc
    15961 tacgctgttg accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca
    16021 gatccagcat tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg
    16081 tctgcaaacc gcgcccgtcg gcatcacgat cggcaaaagt attacgcacc agcgtcagaa
    16141 cgtttttgta ttcacccagc gttttaccca ctaccagcgg aatcgcatct tccagcgttt
    16201 tacggatttt ctcgccgccg ggaatttccc ctacgccagt gtgaccagaa ttatctttga
    16261 taatcacaat attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac
    16321 tgtcatggcc cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag
    16381 aactcatatt tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc
    16441 aaaggtccag ccggggatca ggtactgcat cgggcctgcg tcgttacgcg caccgccagg
    16501 cagacgttta taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag
    16561 accgggcgca tcaggaacgg caatttttcc gtttttaatc tccagcggat tttgggtcag
    16621 gcgacaatcg ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc
    16681 cgccgcgccc acatgggtaa acatcgccag agagatatcg aaatggttat tagaatggca
    16741 gccccaggtc agcccccagt cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt
    16801 ccagaagtgc ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat
    16861 ttcgcgccag ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa
    16921 ctccgccatc acttcacgtc cggagaagcc ctgttctgcg ccacatggat cttcggcata
    16981 ggtaagaaca tcattcagcc ctttgcacaa agaaatggct tcatcaagca gccaggcacc
    17041 gttgggatca acggtaatcc gcgcatccgg gaagtttttc ttcaatgcac gaacagtgtc
    17101 gatttcttgc tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg
    17161 atcctgtgag gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatgtcg
    17221 caactgatac cactcatggt tgcccggtgt attttccaga taaggaagat cggttttagt
    17281 ccgatcaccg atataaaaca gataaccgag gacggtgata gcatcgcgtt gcttgcctgg
    17341 ccctaacagt tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc
    17401 ttccaacgcc gccacggcat taacgcgcaa ttcaaaagtc caggctcctt taccgaaggt
    17461 atcaaaatcg gcggcctgat tacctttatg cacctgctga accactttat tcaggcgcgc
    17521 aacttcctgg cccagcacca tcggaatagc ttcgaccagt gtttgataaa tcacctctcc
    17581 gcccggcgct tcaccaatgc cggtatgccc ggcattatcg gtgagtacca cgatattgcg
    17641 ggtgaagtat gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac
    17701 cggaatgact ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg
    17761 cgacaggttt cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg
    17821 cgattaaggc atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa
    17881 cgatataacc aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca
    17941 ggccaccgga aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca
    18001 gcgccccaat gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa
    18061 cgttgacgta gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg
    18121 ttttacgcgc aatgttcagc gatccggtgc ggcgcatcag ccagtcggaa ataatcccac
    18181 ccagcacacc accgataaaa ccacaaaccg ccggaacgga agccacaaag cccgctttca
    18241 gaatcgacat cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag
    18301 tcagggcgtt gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca
    18361 gttgtttgat ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat
    18421 ccatattgat cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg
    18481 gatgttgatt tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac
    18541 cgcccataaa gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg
    18601 gcgcgaagat caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc
    18661 tttcctgcgc cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt
    18721 cagcaagccc gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa
    18781 agatatcgac gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt
    18841 aaacgcgttt tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat
    18901 aagcccatga gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt
    18961 tggccatttc cgaaccggca atagaaagcg tagcgcggtc accgtagttg aaggatgtga
    19021 cgataaacaa catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg
    19081 cagcctgact taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact
    19141 ctggactgcg acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat
    19201 aggtcgttcg ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc
    19261 catagcggct caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc
    19321 attagccctg gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg
    19381 gttaaaacga agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca
    19441 gtaaaaatcc gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag
    19501 caccgctaaa acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg
    19561 ttcgacccac ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag
    19621 catttcgcca acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc
    19681 attgcagaaa tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg
    19741 ctggaagccc agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc
    19801 gccctgcccg gtcgtggacg taaccaccag aacatactta tcctgatagg gcagccagtc
    19861 gcttaattca ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc
    19921 tgcttcttcc gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc
    19981 cgccataccg ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca
    20041 ctgaactcaa ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc
    20101 tgcatccagg tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc
    20161 gtcaatgaca actgactggc atgcagcatt aaccgctgaa gaccaaaatg ctcagcaccg
    20221 ctgcgattct ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg
    20281 gcaagatgtc ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg
    20341 tagcgcgtgg tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga
    20401 taatgcgtca ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg
    20461 tccagttctt ccaccagtgg ataatccagc accgcttctt ccatcaacca gccgcgcaca
    20521 atcgcatggt aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg
    20581 gcctcgctgg ataatcccat caacaacaca ccagaagttg gtcggtccag acgatgagca
    20641 gtaaaaacat gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg
    20701 cgatccagcc agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat
    20761 tcatcctgat agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt
    20821 tttccaactc agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat
    20881 aatagggtgc aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca
    20941 ttcgcgggat cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg
    21001 gttgggtact attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac
    21061 gcagtaacgc ttcaagcgcc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc
    21121 aacctcaata agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat
    21181 tcacagtgct cccggttcgt ttcgcagcat tccagctaca tttgttgctc cctgctcatc
    21241 cttgacaact tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc
    21301 tcacaccacc ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga
    21361 atcatcctga ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca
    21421 tcctcttcgc ttcatccaga agcctttccc tgaaacacca tcctggtgtg tccttcagaa
    21481 gtgtcatcat cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt
    21541 ctttttcgcg aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga
    21601 aagttaggaa ttaacaagca acatgttgtt tttaaatgaa aacaacgatt cttatctttt
    21661 taagtattcg cttatttcct ggcgatctcc tacagagtct gtaagagatc tctcacaacc
    21721 agatggggta tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg
    21781 aggctaaatg cgtccgttta cgtgttccca gcgtctcttt acacacttca ccggagagat
    21841 tacacaccga gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggtggca
    21901 gttttagcct tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt
    21961 cgtcttcact ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat
    22021 ccccggcaaa ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag
    22081 cgcgttcaac cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg
    22141 catcttcagt agttgaaata atgcacggtg aaggaacgcc gtacagttcc tcacttaacg
    22201 gccagctttt atgttcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg
    22261 cttgtgcggt caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt
    22321 ttagtttatc tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg
    22381 cgggcctgac tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc
    22441 aaggcgttcc acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt
    22501 ttcacggtac ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc
    22561 cgcaggtcgc tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga
    22621 agagttttaa gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg
    22681 tgcgccagac gctggagcgc gacttaagca cttgcgctca gggtaatgtt agcgtggcgt
    22741 tatatcgtct tgatgaactg gaaggccagc cgataggtca tttcaatggc acttgcattg
    22801 atgaccagga tatcactatc gataactatg aattcactac tgactatctg gagaatgcca
    22861 ccagtggtga aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc
    22921 tgatcaccca tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg
    22981 acagagaaaa actgctgcgt tacctggtct ctttccgcca tcacaacgag ttccacgaac
    23041 agtgcgtgga acgcatcttt aatgacctgt tacgcttctg ccagccagag aaattgagcg
    23101 tttacgcacg ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg
    23161 attttgtccc atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc
    23221 tggattcacg cagaaggttg tgaaaggtca tcaggcaggg ctattgtaat caaagggaat
    23281 gacgatattc gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca
    23341 tggatatgtt gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct
    23401 atcaactgtt tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca
    23461 gcaaagaatt gctgtctcgt tttgaaaatt tcgatattaa cgtcttgcgc cgtgaacgcg
    23521 gcgtaaagct ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc
    23581 gcgctttgca ggccaacttg ttcgcggttc tgcgagacat tctcttcgtt tacgggcaaa
    23641 tccataatac cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc
    23701 tggtcttttc catcttgcgt aacgctcgcg cgctgcatgt gggtgaagcg ccaaatatgg
    23761 tggtctgctg gggcggtcac tcaattaacg aaaatgagta tttgtatgcc cgtcgcgtcg
    23821 gaaaccagct gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg
    23881 aagcgccgat gaaaggtgct gcggtcggac acgcgcagca gcgttacaaa gacagtcgtt
    23941 ttattggtat gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg
    24001 aattgatcat catgccggat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg
    24061 gcatcattat cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa
    24121 ttttaatgaa cccggccaac aaagatcagg ttttaccatt gatcctcacc ggcccgaaag
    24181 agagcgccga ctacttccgc gtactggacg agtttgtcgt gcatacgctg ggtgaaaacg
    24241 cgcgccgcca ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa
    24301 aatcgatgcc gctggtgaaa gaaaatcgcc gcgacactgg tgacgcttac agctttaact
    24361 ggtcaatgcg cattgcacca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg
    24421 ctaatctgaa gctttacccg gatcaacctg ttgaagtgct ggctgccgac ctgcgccgtg
    24481 cgttctccgg tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt
    24541 ttggtcctta caaaatcaac ggcgataaag agattatgcg tcgcatggac gacctgctac
    24601 agggttttgt tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg
    24661 aaatctgcac ttaatctccc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg
    24721 gtcacttact catcaactca tttcatttgt tatatgaatg tttcttacca ccctcacgcg
    24781 acaaatatca tcacagttaa tatgtcatac aatttatatt gcaacgcaaa cgtttcccta
    24841 ttttcataaa ccgtatttta tcgctatgaa aaagaaatta tcgccatgat taactaaaag
    24901 tattg
//
LOCUS       DAZUCE010000056        24511 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZUCE010000056 DAZUCE010000000
VERSION     DAZUCE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.056"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            135..224
                     /gene="kdpF"
                     /locus_tag="ACHY03_001906"
     CDS             135..224
                     /gene="kdpF"
                     /locus_tag="ACHY03_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFS3905141.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            224..1897
                     /gene="kdpA"
                     /locus_tag="ACHY03_001907"
     CDS             224..1897
                     /gene="kdpA"
                     /locus_tag="ACHY03_001907"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFS3905142.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1920..3968
                     /gene="kdpB"
                     /locus_tag="ACHY03_001908"
     CDS             1920..3968
                     /gene="kdpB"
                     /locus_tag="ACHY03_001908"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFS3905143.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSVAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRL
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3977..4549
                     /gene="kdpC"
                     /locus_tag="ACHY03_001909"
     CDS             3977..4549
                     /gene="kdpC"
                     /locus_tag="ACHY03_001909"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFS3905144.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4542..7226
                     /gene="kdpD"
                     /locus_tag="ACHY03_001910"
     CDS             4542..7226
                     /gene="kdpD"
                     /locus_tag="ACHY03_001910"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFS3905145.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPQGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7223..7900
                     /gene="kdpE"
                     /locus_tag="ACHY03_001911"
     CDS             7223..7900
                     /gene="kdpE"
                     /locus_tag="ACHY03_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFS3905146.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPVVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8012..8506)
                     /locus_tag="ACHY03_001912"
     CDS             complement(8012..8506)
                     /locus_tag="ACHY03_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905147.1"
                     /translation="MKTKRSLVVRVAFIAATLLLTACAQPEQSSLAGDWLPTHVDKKQ
                     VLEGSAVLKIAPSRCKTNCHGDNLPDNTRRWQVSDEDEKAVIYLHDMSAQEKAGLNPG
                     WQCYTSFFMLACQGKPGTRPAVDENYVSKSGFFVSMLHIGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(8683..10323)
                     /gene="pgm"
                     /locus_tag="ACHY03_001913"
     CDS             complement(8683..10323)
                     /gene="pgm"
                     /locus_tag="ACHY03_001913"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFS3905148.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWSKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(10349..10894)
                     /gene="seqA"
                     /locus_tag="ACHY03_001914"
     CDS             complement(10349..10894)
                     /gene="seqA"
                     /locus_tag="ACHY03_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFS3905149.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            11079..11843
                     /gene="ybfF"
                     /locus_tag="ACHY03_001915"
     CDS             11079..11843
                     /gene="ybfF"
                     /locus_tag="ACHY03_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS3905150.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPTMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            11983..12276
                     /gene="ybfE"
                     /locus_tag="ACHY03_001916"
     CDS             11983..12276
                     /gene="ybfE"
                     /locus_tag="ACHY03_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFS3905151.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            12416..12946
                     /gene="fldA"
                     /locus_tag="ACHY03_001917"
     CDS             12416..12946
                     /gene="fldA"
                     /locus_tag="ACHY03_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFS3905152.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            13156..13242
                     /gene="uof"
                     /locus_tag="ACHY03_001918"
     CDS             13156..13242
                     /gene="uof"
                     /locus_tag="ACHY03_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFS3905153.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            13235..13681
                     /gene="fur"
                     /locus_tag="ACHY03_001919"
     CDS             13235..13681
                     /gene="fur"
                     /locus_tag="ACHY03_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFS3905154.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(13765..14091)
                     /gene="chiQ"
                     /locus_tag="ACHY03_001920"
     CDS             complement(13765..14091)
                     /gene="chiQ"
                     /locus_tag="ACHY03_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFS3905155.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(14141..15547)
                     /gene="chiP"
                     /locus_tag="ACHY03_001921"
     CDS             complement(14141..15547)
                     /gene="chiP"
                     /locus_tag="ACHY03_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFS3905156.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(16125..17789)
                     /gene="glnS"
                     /locus_tag="ACHY03_001922"
     CDS             complement(16125..17789)
                     /gene="glnS"
                     /locus_tag="ACHY03_001922"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFS3905157.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDVEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(18057..20003)
                     /gene="nagE"
                     /locus_tag="ACHY03_001923"
     CDS             complement(18057..20003)
                     /gene="nagE"
                     /locus_tag="ACHY03_001923"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFS3905158.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            20336..21136
                     /gene="nagB"
                     /locus_tag="ACHY03_001924"
     CDS             20336..21136
                     /gene="nagB"
                     /locus_tag="ACHY03_001924"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFS3905159.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            21196..22344
                     /gene="nagA"
                     /locus_tag="ACHY03_001925"
     CDS             21196..22344
                     /gene="nagA"
                     /locus_tag="ACHY03_001925"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS3905160.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            22353..23573
                     /gene="nagC"
                     /locus_tag="ACHY03_001926"
                     /gene_synonym="nagR"
     CDS             22353..23573
                     /gene="nagC"
                     /locus_tag="ACHY03_001926"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFS3905161.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            23621..24373
                     /gene="nagD"
                     /locus_tag="ACHY03_001927"
     CDS             23621..24373
                     /gene="nagD"
                     /locus_tag="ACHY03_001927"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFS3905162.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         5685 a         6344 c         6510 g         5972 t
ORIGIN      
        1 tactaaacat tagttaaatc atggcttttg ccatttttat acttttttta caccccgccc
       61 gcagattttt gcgaaatctt tgcagccaga attctaccct tccggtatca cttttaggcc
      121 actggaggtg cactgtgagt gcaggcgtga taaccggcgt attgctggtg tttttattac
      181 tgggttatct ggtttatgcc ctgatcaatg cggaggcgtt ctgatggctg cgcaagggtt
      241 cttactgatc gccacgtttt tactggtgtt aatggtgctg gcgcgtcctt taggcagcgg
      301 gctggcgcgg ctgattaatg acattcctct tccaggtaca gcgggcgttg aacgtatact
      361 ctttcgttta cctggcgtct ctgaccatga gatgaactgg aagcagtatc tctgtgccat
      421 tctcggcctg aacatgctgg ggctggcggt gctgtttttt atgttgctcg gtcagcacta
      481 tctgccgctt aatccacagc agttgccggg gctgtcgtgg gatctggcgc tgaataccgc
      541 cgtcagcttt gtcacgaata ccaactggca atcttatagc ggtgaaacca cgttgagcta
      601 tttcagccag atggcgggct taacggtgca aaactttctt tctgccgcca gcgggattgc
      661 ggtgattttt gccctcattc gtgcatttac ccgccagagc atgagcacac tcgggaatgc
      721 ctgggtcgat ctgctacgca tcacgttatg ggtgctcgtc cctgtggcgt tggtgatcgc
      781 actgtttttt attcaacaag gtgcgctgca aaactttctg ccctatcagg cagtgaatac
      841 cgttgaagga gcgcaacagc tgttaccaat gggtccggtg gcttctcagg aagcgatcaa
      901 gatgctcggt actaacggcg gtggcttctt taatgccaac tcgtcgcatc cgtttgaaaa
      961 cccaaccgca ctgaccaact tcgtgcagat gctggcgatc ttcttgatcc caacagcgct
     1021 gtgctttgcc tttggtgaag tgacgggcga tcgccgccag gggcgcatgt tgctgtgggc
     1081 gatgtcagtg atttttgtca tctgcgtagg cgtggtgatg tgggcagaag ttcagggtaa
     1141 tcctcatctg ctggcgctcg gcgcagacag cagcatcaat atggaaggta aagagagccg
     1201 tttcggcgtg ctggtcagta gcctgtttgc ggtcgtgact acggcggcgt cctgtggcgc
     1261 ggtgattgcg atgcatgatt cgtttaccgc tctcggtggc atggtgccga tgtggctgat
     1321 gcaaattggt gaagtggtat tcggcggtgt cggttctggt ctttacggca tgatgctgtt
     1381 tgtcctgctg gcggtgttta ttgccgggct gatgattggt cgtacaccgg aatatctggg
     1441 taaaaaaatc gacgtacgtg agatgaaact gactgcactg gcgattctgg tcaccccgac
     1501 gctggtgctg atgggcgcgg cgctggcaat gatgaccgac gccggacgta gcgccatgct
     1561 caaccctggc ccgcatggtt ttagcgaagt gctgtacgcc gtgtcatccg ccgctaacaa
     1621 caacggcagc gcctttgccg gattaagcgc caactctccg ttctggaact gtttactggc
     1681 gttctgcatg tttgtcggtc gcttcggagt gattatcccg gtgatggcaa ttgccggttc
     1741 gctggtaagt aaaaagagcc aacccgccag ctccggcacg ctgccaacgc acggcccgct
     1801 gtttgttggc ctgttaatcg gcaccgtgtt gctggttggc gcactgacct ttatccctgc
     1861 cctggcgctt ggtccggtgg cggaatatct ctcctgatga tattgagtga gcactgaata
     1921 tgagtcgtaa acaactggcg ctattcgaac caacacttgt cgttcaggcg ctgaaagaag
     1981 cggtgaaaaa attaaacccg caggcgcaat ggcgcaatcc ggtgatgttt atcgtctgga
     2041 tcggcagtct gctgaccacc tgtattagca tcgcgatggc aagcggcgcg atgcctggca
     2101 atgcgctgtt tagcgtggcc attagcggct ggctgtgggt caccgtactg ttcgctaatt
     2161 tcgccgaggc gctggcagaa ggccgcagta aagcgcaggc caacagtctg aaaggggtga
     2221 aaaaaactgc ctttgcccgc aagctgcgtg agccgaaata tggcgctgcg gcggacaaag
     2281 ttcctgccga tcaacttcgt aaaggcgata tcgtgctggt agaagctggc gatattatcc
     2341 cctgcgatgg tgaagttatt gaagggggtg catcggtgga tgaaagcgcc atcaccgggg
     2401 aatcggcacc ggtgatccgt gaatccggcg gcgattttgc ctccgtcact ggcggcacgc
     2461 gtattctttc tgactggctg gtgattgagt gtagcgttaa ccccggcgag acatttctgg
     2521 atcggatgat cgcgatggtg gaaggcgcac agcgacgtaa aacgccgaac gagattgccc
     2581 tgaccatcct gctgattgcc ctgactatcg tctttttact ggcaaccgcc acgctgtggc
     2641 cgttttctgc gtggggcggt aatgcagtca gtgtaacggt actggtggcg ctgctggtct
     2701 gtctgatccc aaccactatt ggcggcctgt tgtcagcgat cggcgtcgcc gggatgagtc
     2761 ggatgctggg cgcgaatgtg attgccacca gcggacgtgc agttgaagcg gcaggtgacg
     2821 ttgacgttct gctgctggat aaaaccggca ccatcacact cggtaaccgt caggcatcgg
     2881 agtttatccc tgcgcagggc gtggatgaaa aaacgctggc tgacgccgca caactggctt
     2941 cgctggctga tgaaacgccg gaaggccgca gtattgtgat cctcgccaag caacgtttta
     3001 acctgcgcga gcgcgatgtg cagtcgctcc atgccacctt tgtaccgttt actgcgcaaa
     3061 gccggatgag cgggataaac atcgacaacc gcttgatccg taaaggttct gtcgatgcca
     3121 ttcgccgcca tgttgaggct aacggtggtc acttccctgc cgatgttgat caaaaagtcg
     3181 atcaggttgc gcgtcaggga gccacgccgc tggtggtggt ggaaggttct cgtgtgctgg
     3241 gcgttattgc gctgaaagat atcgtcaaag gcggtattaa agagcgcttc gcccagctgc
     3301 gtaaaatggg cattaaaacg gtgatgatta ccggcgataa ccgtctgact gccgccgcga
     3361 ttgctgcgga agcgggtgtc gatgattttc tcgccgaagc gacaccggag gccaagctgg
     3421 cattgatccg tcagtatcag gcggaaggcc gtttggtggc gatgaccggc gacggcacca
     3481 acgatgctcc ggcgctggcg caggcagatg tcgcggtggc gatgaactcc ggtactcagg
     3541 cggcgaaaga agcgggcaat atggtggatc tcgactctaa cccgaccaag ttgatcgagg
     3601 tggtgcatat tggcaaacag atgctgatga cccgtggctc gctgaccacc ttcagcattg
     3661 ccaacgatgt ggcgaaatac ttcgccatta ttccggcggc gttcgcggca acgtatccgc
     3721 agttaaatgc gctgaacatc atgcgccttc attcgcccga ctccgccatt ctcagtgcag
     3781 tcattttcaa cgccttgatt atcgtctttt tgattcctct ggcgttaaaa ggtgtgagtt
     3841 ataaaccgct taccgcttct gccatgttgc gccgtaactt atggatttac ggtctgggtg
     3901 ggctgctggt gccgtttatt ggtatcaaag tcattgattt actgctgacc gtttgcggtc
     3961 tggtgtgagg tttaccatga gtggattacg tccggcatta tcgacattta tctttctgtt
     4021 attgattact ggcggcgttt acccgctgct gaccaccgtg ctggggcaat ggtggtttcc
     4081 ctggcaggcc aatggttcgt tgattcgtga aggtgatacg gtgcgcggtt cggcattaat
     4141 cgggcagaat tttaccggca acggctattt tcagggtcgc ccgtcggcaa cggcagaaat
     4201 gccttataat ccacaggctt ctggcgggag caatctggcg gtcagtaacc ctgagctgga
     4261 taaactaata gccgcacgcg ttgctgcatt acgggccgct aacccgaatg ccagcacgag
     4321 cgttccggtt gaactggtga cggcgtcggc aagcgggctg gacaataaca tcaccccgca
     4381 agcggcggcc tggcagatcc ctcgcgtagc gaaagcgcgt aatctcagcg ttgaacagct
     4441 cacgcaactg atcgcaaaat acagccaaca accgctggtg aaatatatcg gccagccggt
     4501 cgtcaacatt gttgaactca atctggcgct ggataaactt gatgaataac gaacccttac
     4561 gtcccgaccc cgatcgtctg ctggaacaaa ctgccgcgcc gcatcggggg aagctgaaag
     4621 ttttcttcgg tgcctgcgca ggcgtcggga agacctgggc gatgctggca gaagcccagc
     4681 gactgcgggc gcaagggctg gatattgtgg ttggcgtggt agaaacccac gggcgaaaag
     4741 ataccgccgc catgctggaa gggctggctg ttctgccgtt aaaacgccag gcgtaccgtg
     4801 ggcggcatat aagcgagttt gatctcgatg ccgccctcgc ccgccgcccg gcgctgatct
     4861 taatggacga actggcgcac agtaatgcgc caggttcccg tcatcccaaa cgctggcagg
     4921 atatcgaaga actgctggaa gctggcattg atgttttcac taccgtcaac gttcagcatc
     4981 tggaaagtct gaatgatgtg gtcagcggcg taaccggaat tcaggtacgg gaaactgtgc
     5041 ccgatccttt tttcgatgcc gccgacgacg tggtgctggt ggacttgccc ccggacgatc
     5101 tgcgccagcg gctgaaagaa ggcaaagtct atattgccgg gcaggcggag cgcgccattg
     5161 aacatttttt ccgcaaaggc aatctgatcg ccctgcgcga actggcactg cgccgtactg
     5221 ccgatcgcgt tgatgagcaa atgcgcgcct ggcgggggca tcctggcgaa gagaaagtct
     5281 ggcacacgcg cgacgcgatc cttttatgca tcggccataa caccggcagc gaaaaactgg
     5341 tccgcgcggc ggcgcggctg gcgtcacggc tggggagcgt ctggcacgcg gtgtatgttg
     5401 aaacccctgc cctgcatcgc ttaccggaga aaaaacgtcg ggcaattctc agcgccttac
     5461 gtctggcgca ggagctgggc gcggagacgg caacactttc tgatccagcg gaagagaaag
     5521 cggtagtgcg ttatgcccgt gaacataatc tcggcaagat tattctcggt cgcccagcct
     5581 cgcgccgctg gtggcgtcgg gaaacgtttg ctgaccgact ggcgcgcatc gcccccgatc
     5641 tcgatcaggt gctggtcgcg cttgatgaac cacccgcccg cacgattaac aacgcgccgg
     5701 atagccgctc ttttaaagac aagtggcggg tacagattca gggatgcgtg gttgccgccg
     5761 cgttatgcgc cgttatcacc ttaattgcca tgcagtggct gatggcgttt gatgccgcca
     5821 acctggtgat gctgtatcta cttggcgtgg tggtggtggc gctattttat ggacgctggc
     5881 cttcggtggt tgctaccgtc attaatgtgg tgagtttcga tctctttttt atcgccccac
     5941 gcggcacgct cgccgtctct gatgtgcaat atctgctgac cttcgcggtg atgttaaccg
     6001 tcgggctggt gatcgggaac cttactgctg gcgtgcgtta tcaggcgcgg gtagcccgtt
     6061 accgcgaaca acgcacacgg cacttatatg aaatgtcgaa agctctggct gtgggccgca
     6121 gtccgcagga catcgccgcc accagcgaac aatttattgc ctccacgttt catgcccgca
     6181 gtcaggtgtt gttgcccgat gacaacggta aattgcagcc gttaacacat ccgcaaggaa
     6241 tgacgccatg ggacgatgcc atcgcgcagt ggagttttga taaaggcctg cctgcgggtg
     6301 cgggcaccga cacgttaccc ggcgtgccgt accagatttt gccgttaaaa agcggtgaga
     6361 aaacctacgg gctggtggtg gtggaaccgg ggaatctgcg tcagttgatg atcccggaac
     6421 agcagcgcct gctggagacg tttacgctgt tagtcgccaa tgcgcttgag cggctgacgc
     6481 tgaccgccag cgaagaacag gcgcggatgg caagcgaacg tgaacagatc cgcaacgccc
     6541 tgctggcggc gctttcgcat gatttacgca ctccgctcac ggtgttgttc ggtcaggcag
     6601 aaatcttaac gctcgatctg gcaagcgaag gatcgcccca cgcccgccag gccagcgaga
     6661 ttcgtcagca tgtgctgaac actacccgac tggtgaataa tctactggat atggcgcgaa
     6721 ttcagtccgg cggctttaat ttgaagaaag agtggttaac gctggaagaa gtagtcggca
     6781 gcgcgctgca aatgctggaa ccgggtttat cgtcgcccat caatctttct ctgccagaac
     6841 cgctgacctt aatccacgtt gacgggccac tctttgaacg ggtgctgatt aatctgctgg
     6901 agaacgcggt gaaatatgcg ggtgcgcagg ccgaaattgg tatcgatgcc cacgttgagg
     6961 gcgaaaatct acaactggat gtctgggata acggccccgg tcttccgcaa ggccaggagc
     7021 agacgatatt tgataagttt gctcgcggga ataaagagtc ggcggtaccg ggagtagggc
     7081 ttgggttggc aatttgtcgg gcgatagtgg atgtacacgg cggcaccatt accgcgttca
     7141 accgaccgga aggtggtgcc tgttttcgtg ttacacttcc ccagcaaacc gctcctgaac
     7201 ttgaagaatt tcatgaggat atgtgacaaa cgttctgatt gttgaagatg aacaggctat
     7261 tcgtcgcttt ctgcgcacgg cgctggaggg cgacgggatg cgcgtctttg aggccgaaac
     7321 gctgcaacgc ggcttgctgg aagcggcaac ccgtaagcca gatttgatta ttctcgatct
     7381 cggcctgccc gatggtgatg ggattgagtt tatccgcgac ctgcgccagt ggagcgcggt
     7441 gccggtgatt gtgctttccg cacgcagcga agagagcgac aaaatcgccg cgctggatgc
     7501 cggagcggat gattatctga gtaagccgtt tggcattggc gaattgcagg cccgtctgcg
     7561 cgtcgcatta cgccgccatt cagccactgc cgcgcccgat ccggtagtga aattttccga
     7621 tgttaccgtc gatttagccg cccgcgtgat tcaccggggt gaggaagagg tgcatctcac
     7681 cccgattgaa ttccgcctgc tggcggtgct gctcaacaat gccggaaaag tgctcaccca
     7741 gcgccagctc cttaaccagg tatggggacc aaacgcggtc gaacacagtc attatttgcg
     7801 tatttatatg ggacatctgc gacaaaaact ggagcaagac ccggcccggc ctcgccattt
     7861 tattactgaa actgggattg gttatcggtt tatgctttag gggctgaatt gccggatgcg
     7921 gcgtgagcgc cttatccgac ctacacatcg tcccgtaggc atgataagac gcgtcagcgt
     7981 cgcatcaggc atggcactca tcgcgaaatg tttaatgcgt atttatcgct ttcaattctt
     8041 gctggcaatt ttctgactga cagcgtcgca attcaataat cccgatatgt aacatcgaga
     8101 caaaaaatcc gcttttgctg acataattct catcaacagc cggtcgggtg ccgggcttcc
     8161 cctggcaagc cagcataaaa aatgaggtgt agcattgcca gccgggattt aaaccggcct
     8221 tttcttgtgc tgacatatcg tgaagataaa tcaccgcttt ttcgtcctca tctgacacct
     8281 gccagcgccg ggtattatca ggtaggttat cgccatggca atttgtctta caccgggaag
     8341 gcgcaatttt taaaacagcg ctaccttcta atacctgctt tttatccaca tgcgtcggta
     8401 accagtcgcc agctaatgat gattgttcag gttgggcgca tgcagtcaac agtaatgtgg
     8461 cggcaatgaa tgccactctt acgaccagac tccttttggt cttcattttg aattcccttg
     8521 agctattcag tttgttggca aagtgcgcgt tgtttttgcc ggatgcggcg taaacgcctt
     8581 atccggccta cgaaatcata caaattcaat atattgcacc atcaggcagt catacataaa
     8641 aaagggcgat cttccgaccg ccctttttct tatttaatgt atttacgcgt ttttcagaac
     8701 ttcgctaaca atctcaaccg cttctttctc aatctgcttg cgatgttctt caccgaggaa
     8761 gctttcgcag tagatcttat atgcgtcttc cgtgcctgac ggacgcgcgg cgaaccagcc
     8821 gttgtcagtc atcactttca gaccgccaat agaagcaccg ttgcccggag cagcagtcag
     8881 gcgcgcggtg atcgggtcac ctgccagggt gctggcgctc accatttccg gagacagctt
     8941 agacagcgcc gctttttgtg cggaagtcgc agctgcctgc aaacggttgt agctcggcgc
     9001 accaaagcgt tttgccagtt cgttgtagtg ttcctgcggg ttcttaccgg tgacagcggt
     9061 gatttctgcc gcgagcagac acatgatgat gccgtctttg tcggtggacc acggcgtgcc
     9121 gtcgaaacgc aggaaggaag cccctgcgct ctcttcgccg ccaaagccga agctgccgtc
     9181 gaacagacca tcaacaaacc atttgaaacc taccgggact tctaccagct tgcggcccaa
     9241 gtcattgacc acacggtcga tcatcgcaga agaaaccagc gttttaccga cagcaacatc
     9301 tttgctccac tgcggacgat gctggaacag gtagttgatc gccaccgcca ggtagtggtt
     9361 cggattcatc aaacctgccg gagtgacaat accgtgacgg tcataatccg ggtcgttagc
     9421 aaacgccaga tcgaacttat cacgcagtgc cagcaggcct gccattgcac actcagagga
     9481 gcagtccata cggatcgcac cgtctttatc aaggtgcata aagcggaagg tttgatcgac
     9541 ctgatcgtta acgatagtca ggttgaggtt gtaatactcg ccaatacgct tccagtattc
     9601 gataccggaa ccgcccagcg gatcaacgcc aagcgtcagg cccgctttct gaatcgccgc
     9661 catatcaacg atatcggcca gcccttccac gaacggctgc accagatcct gctctttcac
     9721 atgaccggat gccatcgctt cgtcgaggga gatacgcttc acgcctttca ggccatcggc
     9781 cagcagtgcg ttggcacgat cttccaccac tttagtgacg ttggtatctg ccgggccacc
     9841 atttggcgga ttgtatttga ttccaccatc ttccggcggg ttatgggacg gtgtaatcac
     9901 gataccgtct gccagcgggc cacctttttt attgtgaacc aggatggcat tggaaacggc
     9961 aggcgtcggg gtgaatccat tgttttcctg cacaataaca tctacgccgt tcgctgccag
    10021 cacttccaga acggaaataa atgcaggttc ggacagggcg tgagtatctt tacccacata
    10081 gcaagggcca gtgatgccgt ttttcgcacg ttcttcagca attgcctgag cgatcgccag
    10141 aatgtgcggc tcgttaaagc tgtggcgcgc tgcactgcca cggtgaccgg aagtaccgaa
    10201 tttcaccgcg tgctccgcat tccctgcttc tggtttcagt acataatatt gcgccgtcag
    10261 ttgggcgacg ttaatcaaat cactctgttg tgcaggttgg cctgcacgat tgtggattgc
    10321 cattgctttg tcctttgtct gcaacgtttt agatagttcc gcaaaccttc tcaatcaatt
    10381 ccgccgggaa ttgcatcgac tgcatgatgt gttcgatcat gctgcattta cggccggtgt
    10441 tggtgttggt gatcacccaa tacggcgtgc ctggcacatg tttcggcttg gtctgattac
    10501 catttttcag cagcgtttgt tcatctgccg caaagtaaac gcgcgtacga ccgtgcaacg
    10561 attccgttgc ttcggcaaac gcctgggcgt caagagaata tagtgtagac aacagcagca
    10621 taaagcgatt gaccgctcgc ttttgctctg cgtattcatc cgaaagcaga agttcacgca
    10681 ttgcgcgaac cttgtcttta atcgttttga ccggcttcgc ttcgacgata gcaggtgacg
    10741 caacgcgaac ctctttcgtc accggagcag caggctgtga tgcggcggaa aatttcaaca
    10801 tacgccgtaa aatgtcggat gcgctctcgc cgatatgctt agtgtggctg gcaatatagc
    10861 tgtagagttc atcatcaact tcaatcgttt tcatcttaat ccagtgcagt gtcttagctg
    10921 aatacaagtc gccaggagta tatgaataaa tcccggcagc ggatagcgtc aagcctggct
    10981 tgcagaaaaa ctcggaataa accttatttg ttgcagccct gtggcacaat ggtcaacata
    11041 ataccctaac ccgacaacgc gaaaaaaaga actttgccat gaaattgaat atccgcgcgc
    11101 aaactgcaca aaaccagcac aataattctc ccatcgttct tgtccatggt ctgtttggca
    11161 gcctcgacaa ccttggcgta ctggctcgcg atctggtaaa cgatcacaat atcatccagg
    11221 ttgatatgcg taaccacggt ctttcaccga gagatccggt aatgaattac ccgacgatgg
    11281 cccaggatct tgttgatact ctggatgcac agcagatcga caaagcaaca tttatcggtc
    11341 actccatggg cggtaaagcg gtaatggcac ttactgcact ggcccccgat cgcatcgata
    11401 aactggtggc gatcgatatc gcaccggtcg actatcacgt acgccgtcat gatgagattt
    11461 tcgcggctat caacgcggtc agtgaatcgg acgcacaaac tcgccagcaa gcagcagcaa
    11521 taatgcgcca gcatcttaat gaagaagggg tgattcagtt tctgttgaaa tcttttgttg
    11581 acggggagtg gcgctttaac gtgccggtat tgtgggatca gtatccgcat attgtgggtt
    11641 gggagaaaat cccggcatgg gatcaccctg ccctgtttat ccctggtggt aattctccgt
    11701 atgttagcga gcagtaccgt gatgatttac tggcacaatt tccacaggca cgggcgcatg
    11761 tgattgcagg cgcgggtcac tgggtccatg ctgaaaaacc ggatgcggta ttacgcgcta
    11821 tccgtcgcta tctcaatgat taacgcaact gattaaaaac ccagcgtccg cgtcgctttc
    11881 cgcgcgtggg cgttggcgtg actcatcgcg ctgatgtatt atggcgcgct atccatccgg
    11941 gcagaggcct ggctgattgt ttcccccgaa gtcaccaaga tcatggccaa agaacaaacg
    12001 gaccgtacga cattagatct gttcgcgcac gagcgtcgac cgggacgacc gaaaactaat
    12061 ccgctttcgc gcgatgaaca gctgcgtatt aataaacgca accaactaaa acgcgacaaa
    12121 gtacgtggcc ttaagcgtgt cgaactgaag ctgaacgcgg aagctgtcga ggcgctgaac
    12181 gagctggcgg aatcgcgcaa tatgagccgt agcgaactga tcgaagagat gttaatgcag
    12241 caactggcgg cgctgcgtag tcagggcatc gtttaaattt ccactttcat gtagcacagt
    12301 gtgcagtcct gctcgtttgc tgattccgca agactgcctg ttctgctatg attgccttta
    12361 tccgtgggca attttccacc cccatttcaa taagtttcaa gaggttattt cactcatggc
    12421 tatcactggc atctttttcg gcagcgacac cggtaatacc gaaaatatcg caaaaatgat
    12481 tcaaaaacag cttggtaaag acgttgccga tgtccatgac attgcaaaaa gcagcaaaga
    12541 agatctggaa gcttatgaca ttctgctgct gggcatccca acctggtatt acggcgaagc
    12601 gcagtgtgac tgggatgact tcttcccgac tctcgaagag attgatttca atggcaaact
    12661 ggttgcgctg tttggttgtg gtgaccagga agattacgcc gaatatttct gcgacgcatt
    12721 gggcaccatc cgcgacatca ttgaaccgcg cggtgcaacc atcgttggtc actggccaac
    12781 tgcgggctat catttcgaag catcaaaagg tctggcagat gacgaccact ttgtcggtct
    12841 ggctatcgac gaagaccgtc agccggaact gaccgctgaa cgtgtagaaa aatgggttaa
    12901 acagatttct gaagagttgc atctcgacga aattctcaat gcctgatgtg atgcggcgta
    12961 gactcatgtc tacgccgtat taatagataa tgccaatcaa aataattgct acaaatttgt
    13021 aacttttgct gttgtacctg tacaatgtcc cggtgttcaa gtggccttgc cgttgtaaat
    13081 gtaagctgtg ccacgttttt attaacaata tttgccaggg acttgtggtt ttcatttagg
    13141 cgtggcaatt ctataatgat acgcattatc tcaagagcaa attctgtcac ttcttctaat
    13201 gaagtgaacc gcttagtaac aggacagatt ccgcatgact gataacaata ccgccctaaa
    13261 gaaagctggc ctgaaagtaa cgcttcctcg tttaaaaatc ctggaagtcc ttcaggagcc
    13321 ggacaaccat cacgtcagtg cggaagattt atacaaacgt ctgatcgata tgggtgaaga
    13381 aattggtctg gctacggtat atcgcgtact gaaccagttt gacgacgctg gtatcgtcac
    13441 ccgccacaat tttgaaggcg gtaaatccgt atttgaactg acacagcaac atcaccacga
    13501 tcacctgatc tgcctcgact gcggcaaggt tatcgaattt agtgatgatt ccatcgaagc
    13561 gcgtcagcgt gaaattgccg caaaacatgg cattcgcctg actaaccaca gtctctatct
    13621 ttacggtcac tgtgccgaag gcgattgccg cgaagatgag cacgcgcacg aaggcaaata
    13681 agccagtctg aacgagaaaa gccaacctgt aggttggctt ttttatgcaa gggaaataag
    13741 aaaagcgaga gttacagctc tcacttattt gttattactg cgaatttcct gccagacctt
    13801 atcacaatcg gctttcaccg cctgatcatt tccggtttgc gttgcacgca gacactgctg
    13861 ataatccagt acgcgtacac tttcctggtc ggcaaactgc tgatgttgtt tctctttctt
    13921 cagcacgttt aacacgctct ggcaggcttc aattttttcc ggcgaacctt gtgcggtgtt
    13981 gatacaggcg ctgtatgcct ctttcagacg gctgtcttcc tgtggcgcgg ttgattgcgc
    14041 acaagccacc agccccgatg ccatcatggc gatgagaatc agttttttca tagcaaattc
    14101 ctcaaatgtg cggcaggcac aaaacctgcc gcgtcgggca tcagaagatg gtgaatggtg
    14161 cgattaccat aaatttcacg tcacgctcat cctggaagat gttaccgtaa ccgccacccc
    14221 agcttgggat gtcggagtgg ttgtcgtatt cggtgaagtg caatttgaac atcgtgcctt
    14281 tggcacgacc gtcctgaatg gtgtagaccg catccaggct gtaggcagac tcttcaatag
    14341 tccggttttt gtcgtagtac gcatccggat tgctctgcca ggtcgcaggt ttagcatccc
    14401 atgcgtaaac gtaggaagcg ccgatggcga agcctggaag attccagttt ttcaggtcat
    14461 acatcgcacc gaagaagacc gctttttcgc cgttggcgtt gaagtcagaa cggttatccc
    14521 accagatatc caggcgaccg ttcgaggaag cgtaggttgg agtcatacgt tgcaggaagt
    14581 atccctgctg accgtcagct ttaacccagg tgccttcgag gcgcaaatcg actacgtcag
    14641 ccgcccggta accaaaggtc aacgcttgca gccaggcggt gccgtcataa aggtcgttaa
    14701 cgctgcgatc gtcaacttta tcgcgcgtac cgtagaactg gtagctggtg gttaacgggc
    14761 taccggcgat atcaaatttg tagctggctt tggcaaagta ttgatcgata tacccttccg
    14821 cctgaccaaa tgccgcttcc agtacgaagt tatttttgaa gtcgtatttc gcaccgatgg
    14881 agtgcagata atcaactttg gtagttttat cgttctgata aaactcatcc atttccagat
    14941 gccacggtgc tttgtattcg ttggtccaca tgtaggagaa actcaacgca ccagcatcgc
    15001 cgtaatcaaa attcgctccg gcttccgcac cctgataagt acccggcata aagctccagt
    15061 gaggcgctaa cagcgtctga ccggttggct gaatataacc tgccctcgcc caaaccggac
    15121 cgtatttaaa tttggccgca gctttataca ggcttatacc gcttttgtcg ccggaccagt
    15181 cttcgtcata ggctttatta ctttttgaaa acgcgatttc gttcgggtga gagctgtcgc
    15241 cgttttccgc catttcaatc gccgtaaacg cagcaatatc aaggccgaac atatcagcag
    15301 cataaccgga ctgaaaatcg aggttggcat tccaggtgga gtgagaaagg ttggttttgt
    15361 atttgtcgcc gtcggtaaca tctttacggt cgcgttcacg ctgccagtaa tagataccgc
    15421 cggttaaggt tgaatcgtcg atgaatcctt cggcgcgagc ctccggcatt gccataaagc
    15481 ccgacattgc tgtaacaccg gcgatagcca gcgccagcgt actacgtttg ccactaaacg
    15541 tacgcatggg ttaatcctct ttgacgtata aattgctgca ccaaaggtga acagcgaaaa
    15601 aataaaaatt aaaaaagtag cgttgagaaa agtctcgctg actacaggaa cagactattt
    15661 ttcgcgacgc gaattatcaa tctctttctg taaccagaat gtaagaacat gagcaaccgc
    15721 acatattttg atgatttttg ttacaaggct taagtttgcg gttgaaaggc ttgcagaaga
    15781 ataaaagcgt gaaattccag caagatgaaa gcggttacat tttatttaat tatgttttgt
    15841 gattttaaat ttattttatg gataaattgc gaagcggaat taattcgcga agaaaagcag
    15901 aaaaaacgcc gctgaacgcg gcgtttagga ttgtttgcat atttcttgcc ggatgcggcg
    15961 tgaacgcctt atccggccta caaaagcacg caaactcaat atattgcaga gatcatgtag
    16021 gcctgataag cgtagcgcat caggcaattt agcgtttgaa actgtttgtc tgttccttgc
    16081 cggatgcgac gtaaacgccc catccggcat agcgaaactt aaaattactc gccaactttc
    16141 gcccaggtat cacgcagccc aacggtgcgg ttaaataccg gtttttcggc agtggaatgg
    16201 cggctgtcga ggcagaagta accttcacgc tcaaactgga atgctttacc cgctaccgca
    16261 tctttcagcg acggttcagc aaagccctgt ttgatcacca gcgattccgg gttaatcacc
    16321 gacaggaaat catccgcagc acctgggttc ggcacgctga acagacggtc atacaaacgg
    16381 atttcaaccg gcagcgcatg tgccgcgctc acccagtgaa taacaccttt gactttacga
    16441 ccatctgccg gatctttgct taaggtgtcg gcgtcataag tacagaagat ggtggtgata
    16501 ttaccttcga catctttctc gacgcgttct gccttaatca cataagcatt acgcagacgc
    16561 acttctttac cgagcaccag acgtttgtac tgcttgttag cttcttcgcg gaaatcggcg
    16621 cgatcgatcc aaatctcacc gctaaacggc acctgacggc tgcccatttc cggtttgttc
    16681 ggatggttcg gcatggtaac catttcgcct tcgccctgat agttttcgat aaccagtttc
    16741 accggatcga taaccgccat tgcgcgcggc gcattttcgt tgagatcttc acggatgcag
    16801 gattccagcg acgccatctc aatggtgttg tcctgcttgg tcacgccgat gcgtttgcag
    16861 aactcacgaa tagaagccgc agtgtaacca cgacgacgca gaccggaaat ggtcggcata
    16921 cgcgggtcat cccagccttc aacgtgctta tcggtcacca gcaggttcag cttacgcttg
    16981 gacatcacag tgtattccag attcaggcgc gagaattcgt actggcgcgg gtgaacagga
    17041 atggtgatgt tgtccagtac ccagtcgtac agacgacggt tatcctggaa ctcaagcgta
    17101 cacagagagt gcgtaatacc ttccagggca tcgctgatgc agtgggtgaa gtcgtacatc
    17161 gggtagatgc accacttgtt gccagtctgg tggtgttcag caaatttaat gcgatacagc
    17221 accggatcgc gcatcacgat aaacggtgaa gccatgtcga ttttcgcacg caggcaggct
    17281 ttaccttctt caaaaccgcc ggtacgcatt ttctcgaaca gcgccaggtt ctcttcaacg
    17341 ctacggtcgc ggtacgggct gtttttaccc ggctgtgtca gggtgccacg gtattcgcgg
    17401 atctgttccg gcgtcagttc atcaacgtac gccaggcctt tattgatcag ttcgatcgca
    17461 taggcgtgga gctgatcaaa ataatcggag gagtaacgga cgttaccaga ccagtgaaaa
    17521 cctaaccact caacgtcgtt tttgatcgaa tcgacatact cgatatcttc ttttaccggg
    17581 ttagtgtcgt cgaaacgcag gttgcactgg cctttatagt cctgggcgat cccgaagttc
    17641 aggcagatag atttcgcatg gccaatatgc agatagccat tcggctccgg cgggaaacgg
    17701 gtgtgtactg tggtgtgctt accactggcc agatcttcat cgatgatctg acgaataaag
    17761 ttagtcgggc gggcttctgc ctcactcatc gtggattcct caaagcgtaa acaacgtata
    17821 acggcgtatg atcttataag cgggacaggc tgacaactgt tagtttttta aaattgataa
    17881 tctaacaagg attatgggga tgattgatga aaaaaaaagc ggcagagaag atctccaccg
    17941 cttttgccag tcctgcgtca ttgttggatg cgacgcttaa gcgtcttatc caacctggac
    18001 tgagcttttg taggcctgat aagacgcgtc aagcgtcgca tcaggcataa agcagattac
    18061 tttttgattt catacagcgg tgtttgaccc gccacaacat ggccctgagc tttaatgatc
    18121 aagccactga aatcgtcgat attgctgcaa accaccgggc taatcatcga acgggcgtta
    18181 gcgttcaggt aatccagatc catttccaga atcggttgcc ctgcgcttac ctgcgcgccc
    18241 tcttccacca gacgtttaaa gcctttacct tccagcgcta cggtgtcgat acccatatgg
    18301 acgacgatct ccgcgccttt ttcggtttcc agacagaacg cgtggttggt gttgaagatt
    18361 ttcacgattg tccctgcggc tggtgatacg acgattttat ctgtcggttt caccgccaca
    18421 ccgtcaccca ccgctttgct ggcgaatgct tcgtcaggaa cctgatccag tgccacgaca
    18481 tcaccggtaa tcggcgatac cagctccgcg atagataccg cgtttggtac agcctgcggt
    18541 tttgctacag gagcggcagt tgccggagtt gcttcagcag aggcagctgc taccgggcca
    18601 cgggcaacga ctttcttcat cgcatcgccg atggattctg ctttcgcgcc aacaatcacc
    18661 tgaatagtct gtttgttcag ttttactaca ccagaagcac ccagacgttt acacatcgta
    18721 tcgttaacgc gggcagagtc agccactgta aggcgcagac gggtgataca ggcgtcaatt
    18781 gctttcaggt tgtcagtgcc gccaaccgca gcaatatagt tggtcgccag ttgattgagg
    18841 ccttcttcag tgttgctgtt ggcttcttca gtaacgatct cgtcttcttt atcttcacga
    18901 cccggcgttt tcaggttgaa catgcggata accaaactga acaccacgaa gtagatagcg
    18961 aagaagacaa cacccatcac cagcagcatc cagacgttct ggctggcggc cggcaggtta
    19021 tacatcaacg cgtagtcgat agcccccgca gagaaagaga agccggcatg gatacccagc
    19081 agcgttgcca caaacaggct gataccggtc agcagtgcgt gcaggaggta cagcagcgga
    19141 gcaaggaaca tgaacaggaa ttccagcggc tcagtcacac cggtcaggaa cgcagtaaca
    19201 gcaacagaaa gcagcatccc gccaaccatc ggacgacgct ctttcggtgc tgcgaagtac
    19261 atcgccagcg ccgcacccgg cagaccgaac atcatgatcg ggaagaagcc ggacatgaac
    19321 atccccgcgg tgccgtcacc agcgtagaaa cggttgatgt cgccgtggaa aaccgtaccc
    19381 gccgcgttgg tgaattcacc aatctggaac caggcgatgg tgttcagcac ctgatgcaga
    19441 ccggttggga tcagcagacg gttgatgaaa ccaaagatac cggaacccag cgcgcccgca
    19501 gaaacgatcc actcgccgcc tgcatggata gcgtgctgta ccggcggcca gacgtaacca
    19561 aaaatggccg ccagcaccag acagaagaag ccggtggcga tcggcacaaa gcgtttgccg
    19621 ccgaagaagc tcaggaagtc cggcagttta atatcggacc aacggttata ggctgcgcca
    19681 ccaaccagac cggtaatgat acccgccagt acacccatgt taatttctgg gttgatggtc
    19741 accatcgctt tggttaacac aaagtaacct accgcacccg ccagcgccgc cgcacctgcg
    19801 ctgtctttcg accagctgga tgccacaccg atggcgaaga ttaatgcgag gttatcaaaa
    19861 atcgcaccgc ccgcctgggc aataaacgca acgttaagta aatctggctg accgaatcgc
    19921 agcaacagtg ccgccaccgg cagcaccgcg atagggagct gtaacgccct accgagtcgc
    19981 tggaaaaaac ctaaaatatt catcttattc cccctacgag aaccctattt ggctcgttta
    20041 aagccgtatt tttattgtgc tgcaaattgt actgccgatg ttctgtaatc agattgttag
    20101 atcatctgct acagagtgtg tgaaaattta attcgtatcg caaattaaac gcgtgtcttt
    20161 tgtgagtttt gtcaccaaat atcgttatca tcactccctt ttactggcta aaccagaaaa
    20221 cttattttat cattcaaaaa atcaggtcgg attgacgcct gtctgcgcaa atccaggtta
    20281 cgcttaaaga tgcctaatcc gccaacggct tacattttac ttattgaggt gaataatgag
    20341 actgatcccc ctgactaccg ctgaacaggt cggcaaatgg gctgctcgcc atatcgtcaa
    20401 tcgtatcaat gcgttcaaac cgactgctga tcgtccgttt gttctgggcc tgccaactgg
    20461 cggcacgcca atgaccacct ataaagcgtt agtcgaaatg cataaagcag gccaggtcag
    20521 ctttaagcac gttgtcacct tcaacatgga cgaatatgtc ggtctgccga aagagcatcc
    20581 ggaaagctac tacagcttta tgcaccgtaa tttcttcgat cacgttgata ttccagcaga
    20641 aaacatcaac cttctcaacg gcaacgcccc ggatatcgac gccgagtgcc gccagtatga
    20701 agaaaaaatc cgttcttacg gaaaaattca tctgtttatg ggcggtgtag gtaacgacgg
    20761 tcatattgca tttaacgaac cggcgtcttc tctggcttct cgtactcgta tcaaaaccct
    20821 gactcatgac actcgcgtcg cgaactctcg tttctttgat aacgatgtca atcaggtgcc
    20881 aaaatatgcc ctgactgtcg gtgttggtac actgctggat gccgaagaag tgatgattct
    20941 ggtgctgggt agccagaaag cactggcgct gcaggccgca gttgaaggtt gcgtgaacca
    21001 tatgtggacc atcagctgtc tgcaactgca tccgaaagcc atcatggtgt gcgatgagcc
    21061 ttccaccatg gagctgaaag ttaagacttt aagatatttc aatgaattag aagcagaaaa
    21121 tatcaaaggt ctgtaattgt tatccctgcc ctgacacctt gctcagggca atatttttta
    21181 aaatcggggg tcagaatgta tgcattaacc cagggccgga tctttaccgg ccacgaattt
    21241 cttgatgacc acgcggttgt tatcgctgat ggcctgatta aaagcgtctg tccggtagcg
    21301 gaactgccgc cagagatcga acaacgttca ctgaacgggg ccattctctc ccccggtttt
    21361 atcgatgtgc agttaaacgg ctgcggcggc gtacagttta acgacaccgc tgaagcggtc
    21421 agcgtggaaa cgctggaaat catgcagaaa gccaatgaga aatcaggctg tactaactat
    21481 ctgccgacgc ttatcaccac cagcgatgag ctgatgaaac agggcgtgcg cgttatgcgc
    21541 gagtacctgg caaaacatcc gaatcaggcg ttaggtctgc atctggaagg tccgtggctg
    21601 aatctggtaa aaaaaggcac ccataatccg aattttgtgc gtaagcctga tgccgcgctg
    21661 gtcgatttcc tgtgtgagaa cgccgacgtc attaccaaag tgaccctggc accggaaatg
    21721 gttcctgcgg aagtcatcag caaactggca aatgccggga ttgtggtttc tgccggtcac
    21781 tccaacgcga cgttgaaaga agcgaaagcc ggtttccgcg cggggattac ctttgccacc
    21841 catctgtaca acgcgatgcc gtatattacc ggtcgtgaac cgggcctggc gggcgcgatc
    21901 ctcgacgaag ctgacattta ttgcggtatt atcgctgatg gcctgcatgt tgattacgcc
    21961 aacattcgta acgctaaacg ccttaaaggc gacaaactgt gtctggttac cgacgccacc
    22021 gcgccagcag gtgccaacat tgaacagttc atttttgcgg gtaaaacaat atactaccgt
    22081 aacggacttt gtgtggatga gaacggtacg ttaagcggtt catccttaac catgattgaa
    22141 ggcgtgcgta atctggtcga acattgcggt atcgcactgg atgaagtgct gcgtatggcg
    22201 acgctctatc cggcgcgtgc gattggcgtt gagaaacgtc tcggcacact cgccgcaggt
    22261 aaagtagcca acctgaccgc attcacacct gattttaaaa tcaccaagac catcgttaac
    22321 ggtaacgagg tcgtaactca ataagagaaa gtatgacacc aggcggacaa gctcagatag
    22381 gtaatgttga tctcgtaaaa cagcttaaca gcgcggcggt ttatcgcctg attgaccagt
    22441 acgggccaat ctcgcggatt cagattgccg agcaaagcca gcttgccccc gccagcgtaa
    22501 ccaaaattac gcgtcagctt atcgaacgcg ggctgatcaa agaagttgat cagcaggcct
    22561 ccaccggggg ccgccgcgct atctctatcg tcaccgaaac ccgcaatttc cacgcaatcg
    22621 gcgtacggct tggtcgtcac gacgccacca tcaccctgtt tgatctcagc agcaaagtgc
    22681 tggcagaaga acattacccg ctgccggaac gtacccagca gacgctggaa catgccctgc
    22741 tgaatgccat tgctcagttt attgatagct accagcgcaa actgcgcgag ctgatcgcca
    22801 tttccgttat cctgccagga cttgttgacc cggacagcgg caaaattcat tacatgccgc
    22861 atattcaggt agaaaactgg gggctggtag aagcactgga agagcgtttt aaagtgacct
    22921 gtttcgttgg tcacgatatc cgtagtctgg cgctggcgga gcactacttc ggtgcaagtc
    22981 aggattgcga agactccatt ctggtgcgtg tccatcgcgg aaccggggcc gggattatct
    23041 ctaacgggcg catttttatt ggccgcaacg gtaacgtcgg tgaaattggc catattcagg
    23101 tcgaaccgct gggtgaacgc tgccactgcg gcaactttgg ctgcctggaa actatcgctg
    23161 ccaacgccgc tattgaacaa cgggtgttga atctgttaaa gcagggctac cagagccgcg
    23221 tgccgctgga cgactgcacc atcaaaacta tctgcaaagc cgcgaacaaa ggcgatagcc
    23281 tggcctcgga agtgattgag tatgtcggtc gtcatctggg caaaaccatc gccattgcta
    23341 tcaacttatt taatccgcaa aaaattgtta ttgccggtga aatcaccgaa gccgataaag
    23401 tgctgctccc tgctattgaa agctgcatta atacccaggc gctgaaggcg tttcgtacta
    23461 atctgccggt ggtacgttct gagctggatc accgctcggc aatcggcgct tttgcgctgg
    23521 taaaacgcgc catgctcaac ggtattttgc tccagcattt gctggaaaat taatgtgctt
    23581 ttatagtggc gcttattgtt gtcaatattc tgggtagtcc atgaccatta aaaatgtaat
    23641 ttgcgatatc gacggcgtgc tgatgcacga taacgtcgcc gtaccgggtg cagcggaatt
    23701 tttgcacggg attatggata aaggcctgcc gctggtgttg ctgaccaact atccttcgca
    23761 gaccgggcaa gatctggcga accgctttgc cactgccggt gtcgatgtac ctgacagcgt
    23821 gttttatacc tctgcgatgg cgaccgccga tttcctgcgt cgccaggaag gcaagaaagc
    23881 gtatgtggtg ggcgaaggcg cactgattca tgaactgtac aaagccggtt tcactattac
    23941 cgatgtgaac cctgattttg tgattgttgg cgaaacgcgt tcctacaact gggacatgat
    24001 gcataaagcg gcctatttcg tcgccaacgg cgcacgcttt atcgccacta atccggatac
    24061 tcacggacgc ggtttttatc cagcctgtgg cgcactgtgc gcaggcattg agaaaatctc
    24121 cgggcgcaaa ccgttctatg ttggtaaacc cagcccgtgg atcatccgcg cggcattaaa
    24181 caaaatgcag gcgcactcgg aagaaacggt gattgtcggc gataacctgc gcaccgatat
    24241 tctggcaggc ttccaggcgg gtctggagac gattctggtg ctctctggtg tttcatcgct
    24301 tgacgatatc gacagcatgc cattccgccc cagctggatt tacccgtcgg tcgctgaaat
    24361 cgacgttatc tgaatagcaa ccacgtctgc ggacgtggtt ggttctgatg acaaacgcca
    24421 aactgcctga tgcgctacgc ttatcaggcc tacgccgccc ctgcaatata tttaattttc
    24481 atgcttttgt aggccggata aggcgttcac g
//
LOCUS       DAZUCE010000057        24283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZUCE010000057 DAZUCE010000000
VERSION     DAZUCE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.057"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..465
                     /gene="eilA"
                     /locus_tag="ACHY03_001928"
     CDS             <1..465
                     /gene="eilA"
                     /locus_tag="ACHY03_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HFS3905163.1"
                     /translation="LIILYYTSPLDNAISFALNLNSQNTCNNPIITSILAMFMALKGH
                     NDKAKSLLLKLEPEHGLDYTCVNSLYTKFLIYGASIKNDIMKLLANINTNKINGVILP
                     LIYTVYGKKEYEKRWQQLIKENDLWSNVLLHDPRLLSVKNEFNTIGVMRTSA"
     gene            complement(517..12231)
                     /gene="iatC"
                     /locus_tag="ACHY03_001929"
     CDS             complement(517..12231)
                     /gene="iatC"
                     /locus_tag="ACHY03_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin IatC"
                     /protein_id="HFS3905164.1"
                     /translation="MAGKAHGNGERRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINHAHSDTATSLILPNVKTIPYTLGALETPPTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEVAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDDRTQTNHGIGWRYFTPSWMSGVNMFIDHDLTRYHTRTGIGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDHDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEKPTLSLADSTLSVDLQILLADGKSTSMLTYTARDSS
                     GKPIPGMTLKTQVKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNGNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGAAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAEINGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSGVVEVSGTDKNETGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTTYTAGGVIKVTVTL
                     KDSYENLVGGQRDAINLAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SDWASALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKVNQSSDSKT
                     VNFVADVSTAQIADLVVIKDGSVADGSTANTLRVRVTDAFGNALDGQTVSVLADNSAT
                     VAPTVITEPDGTVEISVTSQTAGVSAVTASINNSSLSRNVTFVADVRTAKIADLEVIK
                     DGSEADGATANTLRARVTDAFGNALAGQTVSVTAGNSATVAPTVITEPDGTVEISVTS
                     QTAGISTVTASINSSSLSRDVTFIADVRTAQIAELVVIKDNSVADGATANTLQVKVTD
                     AFGNTLAGQTVSVTAGNGATVAPTVITEPDGMVEISVTSQTAGASTVTASINSSSLSR
                     NVTFIADVRTAQIADLVVIKDGSEADGSTENTLRARVTDAFGNALAGQTVSVLADNGA
                     TTAPTVITEPDGTVEISVTSQTAGISAVTASINNSTLSQNVMFIADIRTAQIAELVVI
                     KDGSEADGATANTLRARVTDAFGNALAGQTVSVTAGNGATVAPTVITEPDGMVEISVT
                     SQTAGTTAVTASINSSSQSRNVTFIADVRTAKIADLVVTRDNSVADASTANTLQVKVT
                     DANGNTLAGQMVSVMAGNGATVAPTVITEPDGTAEISVTSQTAGISTVTATINSSSQS
                     RNVTFIADVRTAKIADLVVMQDGSVADGTTANTLQVKVTDAFGNALAGQTVSVLADNG
                     ATVALTVTTQPDGTVEISVTSQTAGVSTVTASINNSSQSRNVTFIADVRTAQIADLGV
                     IKDGSVADGSTANTLRARVTDAFGNALAGQTVSVLADNGATVSPAVTTEPDGTVEISV
                     TSQTAGISTVTASINSSSLSRDVTFIADVRTAQIAELVVIKDNSVADGATANTLQVKV
                     TDAFGNTLGGQTVSVTAGNGATVAPTVITEPDGMVEISVTSQTAGASTVTASINSSSL
                     SRNVTFIADVRTAKIADLVVSQDNAVADGATANTLRARVTDAFGNALNGQTVSVLADN
                     GATVALTVTTQPDGTVEISVTSQTAGVSTVTATINNSSQSRNVTFIADVRTAQIADLV
                     VIKDGSVADGAMANTLRVRVTDAFGNALAGQTVSVMADNGATVTPTVITGPDGTVEIS
                     ITSQTAGTSTGTASINNSSLSRNVTFIADVRTAQIADLVVTRDNSVADGATANTLRAR
                     VTDAFGNTLAGQTVSVLADNGATVTPTVTTRPDGTVEFSVTSQTAGTSAVTASINNSS
                     QSRNVTFIADVSTAQIADLGVIKDGSVADGATANTLRARVTDAFGNALAGQTVSVLAD
                     NGATVAPTVITGPDGTVEISVTSQTAGVSAVTASINSSSLSQSVMFIADVRTAQIADL
                     VVTQDGSVADGATANTLRARVTDAFGNALNGQTVSVSADNSAMVTPTVTTQPDGTVEI
                     SVTSQTAGVSTVTASINSSSLSRNVTFVADVSTAKIADLVVIQDNSVADGAMANTLRV
                     RVTDAFGNALAGQTVSVLADNGATVAPVVTTQPDGTVEISVTSQTAGISAVTATINSS
                     SLSRNVTFIADVRTAQIADLEVTQDNAVADGSTANTLQVRVTDANGNALAGQTVSVTA
                     DNSAMVASTVITGPDGTVEISVTSQTAGSSTVTATINSSSQSRNVTFVADVRTAKIAD
                     LVVTQDGSVADGATANTLRVRVTDAFGNALGGQTVSVLADNGATVAPVVTTQPDGTVE
                     ISVTSQTAGVRTVTASINNSSQSRDVTFIADVRTAQIADLVVIKDGVVADGAMANMLR
                     VRVTDANGNTLGGQTVSVMADNGAAVASTMTTKPDGTVEISVTSQTAGTSAVTASINS
                     SSQSRDVTFIADVRTAQIADLVVIKDGSVADGAMANMLRARVTDAFGNALAGQTVSVF
                     AGNGATTAPTVTTQPDGTVEISVTSQTAGVSAVTASINSSSQSRDVTFIADVRTAKIA
                     DLVVIKDGSVADGAMANMLQVKVTDANGNVLAGQTVSMMAGNGATVAPTVITEPDGTV
                     EIPVTSQTAGASAVTASINSSNASRNVTFVADVRTAKIADLVVIKDGSVADGAMANTL
                     QVKVTDAFGNALGGQTVSVTAGNSATVTPTVTTQSDGTVEFSVTSQTAGVSAVTATIN
                     NHSLSQNVTFVPGDASQLTSTVETNKSNYTVGETITITVTLRDAFDNLVTGAASQLAA
                     NSVLTVDGTDPSETGSWVESGGVYTTTRMATIAGTDQHANLQLQTWSDGVTSDRYDIQ
                     SGSPAQATSTIATDKNAYIAGETITVAVTLKDAHGNLVEGGESLLSGDNVTVEGAVRS
                     GGWSETAGVYTATWSAQMAGDSHHATLTLPEWGGSKQSESYSIHSGAPVQANSAIRTD
                     KSAYIAGDPLTVTVTLRDEFGNPALGLTSEVIESYIDNFAVGGATPDSLYWVEQNSGE
                     YTIVWTAWVAEENLVASLKLKTWAMEIKSSLYGIQPGAAAQTQSTIVTDKTKYIAGDS
                     ITVTVVLKDAQGNFITDGVAQLNEENVQVRNADSIQGNNWVYNGDGKYQRQYMAHFAE
                     ANLNAQLKMAGWSDANYSKNYTINRGEVSMFRSQLRIHEVLVVAGADIPVSVLLADEF
                     GNPVNDGLDLLTDDAVYLQNVEKKEGTKWVSVGEGRYERTYRAYKEGENLNSYLHING
                     WYVNGQPSYTILPFVEVESLSVNGVRFRATDGFPETGFDGAKFTLLLTHNMRNTDYNW
                     TAGIYGINVDSNGEVTLSLLIRSEVTITGKPKNGKGNDVVFKFKIKKWFTSLGAASSN
                     TWDIINASCSYGQMPSSLELAQRPSGGVVPRKVGTLWGEYGNLKTYGNAFSGTDYWTT
                     TQLLGVHEKFNPETGISELGTGKSSGLCVEYY"
     gene            complement(12433..13617)
                     /gene="nepI"
                     /locus_tag="ACHY03_001930"
     CDS             complement(12433..13617)
                     /gene="nepI"
                     /locus_tag="ACHY03_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFS3905165.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            13851..14204
                     /locus_tag="ACHY03_001931"
     CDS             13851..14204
                     /locus_tag="ACHY03_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3905166.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     INTKELTEICYDCKN"
     gene            14188..14511
                     /locus_tag="ACHY03_001932"
     CDS             14188..14511
                     /locus_tag="ACHY03_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3905167.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LDVLL"
     gene            complement(14627..15079)
                     /gene="yicN"
                     /locus_tag="ACHY03_001933"
     CDS             complement(14627..15079)
                     /gene="yicN"
                     /locus_tag="ACHY03_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFS3905168.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(15132..16466)
                     /gene="adeQ"
                     /locus_tag="ACHY03_001934"
                     /gene_synonym="yicO"
     CDS             complement(15132..16466)
                     /gene="adeQ"
                     /locus_tag="ACHY03_001934"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFS3905169.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            16641..18407
                     /gene="adeD"
                     /locus_tag="ACHY03_001935"
     CDS             16641..18407
                     /gene="adeD"
                     /locus_tag="ACHY03_001935"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFS3905170.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAKQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFMPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(18455..19846)
                     /gene="uhpT"
                     /locus_tag="ACHY03_001936"
     CDS             complement(18455..19846)
                     /gene="uhpT"
                     /locus_tag="ACHY03_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFS3905171.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(19984..21303)
                     /gene="uhpC"
                     /locus_tag="ACHY03_001937"
     CDS             complement(19984..21303)
                     /gene="uhpC"
                     /locus_tag="ACHY03_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFS3905172.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(21313..22815)
                     /gene="uhpB"
                     /locus_tag="ACHY03_001938"
     CDS             complement(21313..22815)
                     /gene="uhpB"
                     /locus_tag="ACHY03_001938"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFS3905173.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(22815..23405)
                     /gene="uhpA"
                     /locus_tag="ACHY03_001939"
     CDS             complement(22815..23405)
                     /gene="uhpA"
                     /locus_tag="ACHY03_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFS3905174.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(23567..>24283)
                     /locus_tag="ACHY03_001940"
     CDS             complement(23567..>24283)
                     /locus_tag="ACHY03_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905175.1"
                     /translation="LLADTERQVLEGSSFVLCCGEKINGTISELLRKKITDSTHSIKS
                     LTLSESPSYEVYEEIFKKAVFTPDECHLVRDSLVQDGIKPEKIAKIISCLSSRTGIGN
                     LFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSLVALNGRSYHQHVDFSEYGFTQ
                     TLCMLGAVVNNANDTFDVANYIYTTKGENIIDNDAEDDSPYVHSDESYRHKDDYWSRD
                     NDIRNLRRNFSGNITIATYK"
BASE COUNT         5749 a         7010 c         5896 g         5628 t
ORIGIN      
        1 ttaattatac tttattatac atcaccactc gataacgcga tatcttttgc attaaatctt
       61 aacagtcaaa acacctgtaa caatccaatt attacatcta ttctggctat gttcatggca
      121 ctgaaaggac ataatgacaa agccaaaagt ttattgctta aacttgaacc cgaacatggt
      181 cttgattaca cctgcgttaa ctcgctttat acgaagtttc tgatctatgg cgcatctata
      241 aaaaatgaca ttatgaagtt actggcaaat attaacacta acaaaatcaa tggtgttatt
      301 ttgccgttaa tttacactgt ttatggtaaa aaagaatatg aaaagcgctg gcaacaatta
      361 attaaagaga atgatttgtg gtcaaatgtt ttgcttcatg acccccgttt gctcagtgtg
      421 aaaaatgaat ttaatactat tggtgtgatg cgtacttctg cgtaagaact aatataaatt
      481 gccctgctga tgcagggcaa tttagtgatt acttcgttaa taatactcta cacacaagcc
      541 agaagacttt cctgtcccaa gctctgatat accagtttca ggattaaatt tctcgtgtac
      601 ccctaagagc tgagtggttg tccaataatc cgtaccgcta aaagcgttcc cataagtttt
      661 caaattacca tattcgcccc acaacgtacc tacttttcgc ggtactaccc caccgcttgg
      721 ccgctgcgct aattctaaag atgacggcat ttgaccatag ctgcatgaag catttatgat
      781 atcccaggta ttactactgg cagcacctaa actagtaaac cattttttaa tcttgaattt
      841 aaatacaaca tcattccctt taccattctt gggtttcccg gtaatagtaa cttcacttct
      901 tattagcaga gataatgtta cttctccatt actgtcaaca ttaattccat aaatcccagc
      961 cgtccaatta taatccgtat ttctcatatt atgggttagt aacagcgtga attttgcacc
     1021 atcaaaccct gtttctggaa aaccatctgt cgccctgaat cttacaccat taacactcag
     1081 cgactcgact tcgacaaaag gtaggatggt ataagacggt tgcccgttta cataccaacc
     1141 attaatatgt agatatgagt ttaagttttc tccttctttg taggccctat aggtacgctc
     1201 atatctgcct tcacccactg acacccattt agtcccctct ttcttttcca cattttgtaa
     1261 gtacactgca tcatctgtca gcaaatccag accgtcattc acaggattac caaattcatc
     1321 agctaataaa acactgacag gtatatccgc cccagctacc actaaaactt cgtgtatacg
     1381 aagctgtgaa cgaaacatac ttacttcacc tctgttaata gtgtagtttt ttgaataatt
     1441 ggcatcgctc caaccagcca tcttcagttg cgcattcaag ttcgcttccg caaaatgcgc
     1501 catatactgt ctttgatatt taccatcgcc gttgtaaacc caattattgc cttgaataga
     1561 atcagcgttc ctgacttgta cgttttcctc attaagctgc gcgacaccat cagtaataaa
     1621 gttaccctgg gcatctttaa gaacaacggt caccgttatg ctatccccag cgatatattt
     1681 cgttttgtcc gtgacaatcg tggattgagt ttgtgccgcc gcacctggtt gtattccgta
     1741 taatgaggac ttaatctcca ttgcccatgt ttttaatttc agactggcaa ccagattttc
     1801 ctcggcaacc catgctgtcc agacaatggt atattcacca ctattctgct cgacccaata
     1861 cagagaatcg ggagttgcgc cgcctaccgc gaaattatcg atatatgact cgatgacttc
     1921 tgatgtcagc cctaaagcag gattaccaaa ctcatcccgc aacgttaccg tgacggttaa
     1981 tggatccccg gcgatatatg ccgatttatc tgtcctgata gcagaatttg cctgcaccgg
     2041 agcaccactg tggatggaat aactctctga ttgtttactg ccgccccact caggcaacgt
     2101 cagcgtcgcg tggtgagagt ctcccgccat ttgcgccgac catgtagcag tataaacacc
     2161 ggcagtttca gaccatcctc ctgaacgtac ggctccttct actgttacgt tatcaccaga
     2221 cagtaaggac tcgccccctt caaccaggtt accgtgcgcg tctttcagcg tcacggcgac
     2281 ggttatagtc tcgccagcga tgtatgcgtt tttatctgtg gcgatagttg atgtggcttg
     2341 cgccggagaa ccggactgta tgtcatagcg atctgacgtt acaccatcac tccacgtctg
     2401 cagttgcagg ttagcgtgct gatcggtacc ggcaatcgtc gccattcggg ttgtggtata
     2461 cacgccacct gattcaaccc agcttcccgt ttctgacggg tcggtgccat ccaccgtcag
     2521 cacactatta gcggctaact gtgaagctgc accggttacc agattatcga acgcatcccg
     2581 gagcgttacc gtgatggtga tggtctctcc aaccgtgtag ttagacttat ttgtttcaac
     2641 ggttgaagtg agctgcgatg cgtcacccgg tacaaaggtg acgttctggc tcaggctatg
     2701 attgttgatg gttgctgtga ctgcgctaac tcccgccgtc tgactggtga cactgaactc
     2761 caccgtaccg tccgactgcg tggtgaccgt cggagtgacc gttgcgctgt tgcctgccgt
     2821 cacgctgacc gtctgtccgc caagcgcatt gccgaacgca tcggtgacct tcacctgcag
     2881 cgtgttcgcc attgcgccgt ctgccactga gccgtcctta atgaccacca gatccgcgat
     2941 cttcgccgtc ctgacatcgg caacaaacgt cacgttccgg ctcgcgttgc tgctgttgat
     3001 actggcggtg accgcactgg ctcccgccgt ctggctggtg acagggatct ccaccgtacc
     3061 gtccggctct gtaatcaccg tcggggcaac cgttgcaccg ttgcctgcca tcatgctgac
     3121 cgtctgcccg gcaagcacat tgccgttagc atcggtaacc tttacctgca gcatgttcgc
     3181 cattgcgccg tcagccactg agccgtcctt aatgaccacc agatcagcga tcttcgccgt
     3241 tctgacatcg gcgataaacg tcacatcccg gctctggctg ctgctgttaa tgctggcagt
     3301 gaccgcactg actcccgccg tctggctggt gacagagatc tccaccgtgc cgtccggctg
     3361 cgtggtgacc gtcggagcag tcgtggcacc gttgcctgcg aacacgctga ccgtctgccc
     3421 ggcaagtgca ttaccgaacg catcggtgac cctcgcccgc agcatattcg ccattgcgcc
     3481 gtcagccact gagccgtcct taatgaccac caggtccgcg atctgcgccg ttctgacatc
     3541 ggcgataaac gtcacatccc ggctttgact gctgctgttg atgctggcgg tgaccgcact
     3601 ggtgcccgcc gtctggctgg tgacactgat ctccaccgtg ccgtccggct ttgtggtcat
     3661 cgtcgaagca accgctgcac cgttgtccgc catcacgctg accgtctgtc cgccaagcgt
     3721 attaccgtta gcatcggtga ccctcacccg cagcatgttc gccattgcgc cgtctgccac
     3781 tacgccgtcc ttaatgacca ccagatcagc gatctgcgcc gtcctgacat cggcgataaa
     3841 tgtcacgtcc cggctctggc tgctgttgtt gatgctggca gtgacagttc tgactcccgc
     3901 cgtctgactg gtgacagaaa tctccaccgt gccgtccggc tgcgtggtga ccaccggagc
     3961 gaccgttgcg ccattgtccg ccaacacgct gaccgtctgc ccgcccagcg cattaccgaa
     4021 cgcatccgtc accctcaccc gcagcgtgtt cgccgtcgca ccgtctgcca ctgagccgtc
     4081 ctgcgtcacc accagatccg cgatcttcgc cgtcctgaca tcggcgacaa acgtcacgtt
     4141 ccggctctga ctgctgctgt tgatggttgc ggtgactgta ctgctccccg ccgtctgact
     4201 ggtgacactg atctccaccg taccgtccgg ccctgtgatg acagtcgaag cgaccattgc
     4261 actgttgtct gccgtcacgc tgaccgtctg cccggcaagc gcattaccgt tagcatcggt
     4321 gaccctcacc tgcagcgtgt tcgccgtcga accgtctgcc acggcgttat cctgcgtcac
     4381 ctccagatcc gcgatctgcg ccgtcctgac atccgcaata aacgtcacgt tccggctcag
     4441 gctgctgctg ttgatggttg cagtgactgc actgattccg gcggtctgac tggtgacaga
     4501 gatctccacc gtaccgtccg gctgcgtggt gaccaccgga gcgaccgttg cgccgttatc
     4561 cgccaacacg ctgaccgtct gtccggcaag tgcattcccg aacgcatcgg tcaccctcac
     4621 tcgcagcgtg ttcgccattg cgccgtcagc cactgagtta tcctggataa cgaccagatc
     4681 cgctattttt gccgtgctga catcggcgac aaacgtcacg ttccggctca ggctgctgct
     4741 gttgatgctg gcggtgacag tactgactcc cgccgtctga ctggtgacac tgatctccac
     4801 cgtgccatcc ggctgcgtgg tcaccgtcgg agtgaccatt gcgctgttgt ctgccgatac
     4861 gctgaccgtc tgcccattca gcgcattccc gaatgcatcg gtcaccctcg cccgcaacgt
     4921 attcgccgtc gcaccgtctg ccactgagcc gtcctgcgtc accaccagat ccgcgatctg
     4981 cgccgtcctg acatcggcaa taaacatcac actctggctc aggctgctgc tgttgatact
     5041 ggcggtgacc gcactgactc ccgccgtctg gctggtgaca gagatctcca ccgtgccgtc
     5101 cggccctgtg atcaccgtcg gggcaaccgt tgcgccgttg tccgccaaca cgctgaccgt
     5161 ctgcccggca agcgcattcc cgaacgcatc cgtcaccctc gcccgcagcg tgttcgccgt
     5221 tgcgccgtct gccactgagc cgtccttaat aacccccaga tccgcgatct gcgccgtgct
     5281 gacatcggcg ataaacgtca cgttccggct ctggctgctg ttgttgatgc tggcggtgac
     5341 cgcactggtg cccgccgtct ggctggtgac actgaactcc accgtgccgt ccggccgcgt
     5401 ggtgaccgtc ggggtgaccg ttgcaccgtt gtccgccaac acgctgaccg tctgtccggc
     5461 aagcgtatta ccgaacgcat ccgtcaccct cgcccgcagc gtgtttgccg tcgcgccgtc
     5521 tgccactgaa ttatcccggg taaccaccag atcagcgatc tgcgccgtcc tgacatccgc
     5581 aataaacgtc acgttccggc tcaggctgct gttgttgatg ctggcagtgc ctgtactggt
     5641 tccggctgtc tggctggtga tactgatctc caccgtcccg tccggccctg tgatgacagt
     5701 cggagtgacc gtagcaccgt tgtccgccat cacgctgacc gtctgcccgg caagcgcatt
     5761 accgaacgca tccgtgaccc tcacccgcag cgtgttcgcc attgcgccgt ctgccactga
     5821 gccgtcctta atgaccacca gatccgcgat ctgcgccgtc ctgacatcgg cgataaacgt
     5881 cacgttccgg ctctggctac tgttgttgat ggttgcagtg acagtactga ctcccgccgt
     5941 ctggctggtg acactgatct ccaccgtgcc gtccggctgc gtggtgaccg tcagagcgac
     6001 cgttgcgccg ttatccgcca acacgctgac cgtctgccca ttcagcgcat tcccgaacgc
     6061 atcggtgacc ctcgcccgca gcgtgttcgc cgtcgcgccg tctgccaccg cgttatcctg
     6121 gctaacgacc agatccgcta tcttcgccgt cctgacatcc gcaataaacg tcacgttccg
     6181 gctcaggctg ctgctgttga tgctggcagt gacagtactg gctcccgccg tctgactggt
     6241 gacactgatc tccaccatac cgtccggctc tgtaatcacc gtcggggcaa ccgttgcgcc
     6301 gttgcctgct gtcacactga ccgtctgccc gccaagcgta ttaccgaatg catcggtgac
     6361 cttcacctgc agcgtgtttg ccgtcgcgcc gtctgccact gagttgtcct taatgaccac
     6421 cagctccgcg atctgcgccg tcctgacatc cgcaataaac gtcacatccc ggctcaggct
     6481 gctgctgttg atgctggcag tgacagtact gatccccgcc gtctggctgg tgacactgat
     6541 ttccaccgtg ccgtctggtt ctgtggtgac cgccggagac accgttgcgc cgttgtccgc
     6601 caacacgctg accgtctgcc cggcaagcgc attcccgaac gcatcggtga ccctcgcccg
     6661 cagcgtgttc gccgtcgaac cgtctgccac tgagccgtcc ttaataaccc ccagatccgc
     6721 gatctgcgcc gtcctgacat cggcgataaa cgtcacgttc cggctctggc tactgttgtt
     6781 gatgctggcg gtgacagtac tgactcccgc cgtctggctg gtgacactga tctccaccgt
     6841 gccgtccggc tgcgtggtga ccgtcagagc gaccgttgcg ccgttatccg ccaacacgct
     6901 gaccgtctgt ccggcaagcg cattcccgaa cgcatcggtg accttcacct gcagcgtatt
     6961 cgccgtcgtg ccatctgcca ctgagccgtc ctgcatcacc accagatcag cgatcttcgc
     7021 cgtcctgaca tcggcaataa acgtcacgtt ccggctctgg ctgctgctgt tgatggttgc
     7081 agtgacagta ctgatccccg ccgtctggct ggtgacactg atctccgccg tgccgtccgg
     7141 ctctgtaatc accgtcgggg caaccgttgc accgttgcct gccatcacgc tgaccatctg
     7201 cccggcaagc gtattaccgt tagcatcggt gaccttcacc tgcagcgtat tcgccgtcga
     7261 agcgtctgcc actgagttat cccgggtgac caccaggtcc gcgatcttcg ccgtcctgac
     7321 atccgcaata aacgtgacgt tccggctctg gctgctgctg ttaatgctgg cagtgaccgc
     7381 agtggtgcct gccgtctggc tggtgacact aatttccacc ataccgtccg gctctgtaat
     7441 caccgtcggg gcaaccgttg cgccgttgcc tgctgtcaca ctgaccgtct gcccggcaag
     7501 cgcattgccg aacgcatcgg tcaccctcgc ccgcagcgtg ttcgccgtcg caccgtctgc
     7561 ctctgaacca tccttaataa ccaccagctc cgcgatctgc gccgtcctga tatcggcgat
     7621 aaacatcacg ttctggctca gggtgctgtt gttgatgctg gcagtgactg cactgatccc
     7681 cgccgtctga ctggtgactg agatctccac cgtaccgtcc ggctctgtga tgaccgtcgg
     7741 ggcagtcgtt gcgccgttgt ccgccaacac gctgaccgtc tgcccggcaa gcgcattgcc
     7801 gaacgcatcg gtcaccctcg cccgcagcgt gttctccgtc gaaccgtccg cctctgaacc
     7861 atccttaatg accaccagat ccgcgatctg cgccgtcctg acatccgcaa taaacgtcac
     7921 gttccggctc aggctgctgc tgttgatgct ggcagtgaca gtactggctc ccgccgtctg
     7981 gctggtgaca ctgatctcca ccataccgtc cggctctgta atcaccgtcg gggcaaccgt
     8041 tgcgccgttg cctgctgtca cactgaccgt ctgcccggca agcgtattac cgaatgcatc
     8101 ggtgaccttc acctgcagcg tgttcgccgt cgcgccgtct gccactgagt tgtccttaat
     8161 gaccaccagc tccgcgatct gcgccgtcct gacatccgca ataaacgtca catcccggct
     8221 caggctgctg ctgttgatgc tggcagtgac tgtactgatc cccgccgtct ggctggtgac
     8281 actgatttcc accgtcccgt ccggctctgt aatcaccgtc ggggcaaccg ttgcgctgtt
     8341 gcctgcagtc acgctgaccg tctgcccggc aagcgcattt ccgaatgcat cggttaccct
     8401 cgcacgcagc gtgttcgccg tcgcaccgtc cgcctctgaa ccatccttaa tgacctccag
     8461 atcagcgatc ttcgccgtcc tgacatcggc aacaaacgtc acgttccggc tcaggctgct
     8521 gttgttgata ctggcggtga ccgcactgac tcccgccgtc tggctggtaa cagagatctc
     8581 caccgtcccg tccggctcgg tgataaccgt cggagcgacc gttgcgctgt tgtccgccaa
     8641 cacgctgacc gtctgcccgt ccagcgcatt accgaacgca tccgtcaccc tcacccgcag
     8701 cgtgttcgcc gtcgaaccgt ctgccactga gccatcctta atgaccacca gatcagcgat
     8761 ctgcgccgtg ctgacatcag caacaaagtt caccgtttta ctatcgctgc tttgattgac
     8821 cttagcctca acagtgctgg catccgctac ggtactgctc agatcaacct ctgccatacc
     8881 atgcgcatcc gtcctgatat ctgcgctctg ccctgcattg gcaaatacag ccgccccctt
     8941 cgtcaccgta aaggtgacgg tctgctccgg cagcacattg ccaaactgat cttccacccg
     9001 gacattcacc gtatgtcgat cgctgccatt cgccagcaca tccgggttac ccgttgtcac
     9061 ttgcatcgcg acaatttgtg cgctggcggc atcgccacta atactgtaat catttgaagt
     9121 cagagcgctc gcccagtcgg acatctgtag ctgtgctttt aaaccggtgc cagggagtaa
     9181 ggcggtatac gttgcggtat aaattccttt ttgatcttca ttccaggcga tgctttccgc
     9241 ttttgtattt ggcagctgga tagccaggtt gatagcgtct cgttgccccc cgaccaggtt
     9301 ttcatagcta tccttgagtg ttaccgtaac ttttatcacg ccgcccgcgg tgtaggtcgt
     9361 tttgtcggta acgatggagg aataagccag cacagctccg ctttcgcgga tggcatacgc
     9421 atcggattgc tgcgcgctac tccatgtcga tagtttcagc gtggcgtaat ggcgatcgcc
     9481 agcaatcttc gcggttcggg tggtggtata aacgccgtca gactcttcgc tccagttacc
     9541 cgtttcattt ttatctgtac cgctcacctc gaccacaccg ctacccgcga gcagcgaagc
     9601 catccctttc accaggtttt tctgctcatc cataagggtg acggacactt ttatcttgcc
     9661 acccgcagtg tatgacacat cgtccgtctt gatggtggag gtcagctgcg agatatcgcc
     9721 agtgataaag ctcacttcca cagactgact actgccattg atctcggcct tgataagact
     9781 gtttaccgcc tggtcactgg caaggttaac gctggctatc ccgtctttat ctgtcgtcac
     9841 aataccgcca ttcggatcgg caaaaaccgc cgcaccattc acgaccgtga aggtaacatt
     9901 ttgtccctgc agtaaatttt gatgcacatc cctgaccgtc acagcgacgg tattgtgatc
     9961 gcgtctggca actgcgttat tcgcggtgat ctgcatcgca gcgattttgg cggtatcttt
    10021 atcacccgca atactgaacg catccgaatg tttggtttgc cagccgggca tcgtcagctg
    10081 ggccgttaat ttgtcgccaa tgagatgggc cgtatagctt gctttgtaaa ttccctcact
    10141 ttcttccgtc caggcactaa cggcatcagc cttcgtgttg tctactttaa cggtttgttt
    10201 gagaagttct tttcgtccgg taaccccgtt gccgttatca tctctcaggg tcactttaac
    10261 gactatcggc ttacccgcga cgtaattatc ctggtctgtc tcaatcgtgg agtcagcgcg
    10321 ggatgccgta ttcgccacga tagcaatcag cgctggcgtc ttagcgatat cgtcgccgtt
    10381 aaattgcggc atcagcgata acgcgccaga cgtcttcccg gcggtaacta tttgggtata
    10441 ggttccgtta ccgttgtctt tccattcgct taaggcgaaa tcctgcaaac ctttcacctg
    10501 tgtttttaac gtcatacctg ggattggctt gccgctggaa tctcgcgcgg tataggtcag
    10561 cattgaggtt gattttccat cagcaagcag gatttgcaga tcgacagaaa gcgttgaatc
    10621 ggcgaggctt aatgtgggtt tttccacgac caccatactt tcttcacgcc gggagaaatt
    10681 tcctttcgaa tcttccgctg taaccgctat cgggtaagtg ttgtcggttt caggcattgc
    10741 cgtgaatcgg tagggaggaa tagtgacctg aatatccttg ccggaaacag ctacttttcc
    10801 gcccgctgat tgcagggagg cggcttcaat gtcataacct ttcagcgcat atttggtttg
    10861 taaggaagaa accagcgatt taacctcacc cggcttgcct ttcaacggat cggtcagcgt
    10921 cagccgcacc agctctttct tgcgatattc gaggacgata ttgttattac gatcgaccag
    10981 gtcatagcga ctacccacaa ggctgcgccg agctgcaact tcagctggat cgagctgttt
    11041 ttgcagtgaa tgaccaggtt gcaacgtcaa ctccatacca attcgggtgt cgttttctcc
    11101 ctgtttgccc tgccgttgct cggcactaaa ggagattaag ggaacggggg tgtaactcaa
    11161 acctgcggta atcgcgtgag gatcattttg tcgctcgtct ttaccaaaca gcgccacttc
    11221 atcgccataa tattgttcat agaccagctt gccgcccagc tgcggccagg ccggtaacca
    11281 gccttcggcg cgaagatccc aaccgttcgc tggacgtgct tcatagtcat ggtcgagttc
    11341 cggtgcacta cgccagttac tcaggcgcag ataaccgttg ccgctcagtt tcaggtaatc
    11401 gcgccagtat tcaacgccta tcccggttcg ggtgtgatag cgggtcaggt catgatcgat
    11461 aaacatattg acgccagaca tccagctcgg ggtgaaataa cgccagccga taccgtggtt
    11521 ggtttgagtg cgatcatccg tacggtgaag tgtgtgctgg ctgaagacca ggttatccgg
    11581 cgtttcgtac cagggatgga gaaattcgaa tgaagagctt ttaaggctga aatcttcatc
    11641 cacaccaaga gatacccggg cagttcccca acgactgagc cagtccgttg cctgcgcaga
    11701 tgcagaagaa gccacccagc cacgcgccat actcgccgcc tgttcgctgt ccatatcgcg
    11761 agctaaaagt gttccgcttt gctgcgccac ttccgcgacc tgcatttgcg gatctttgcc
    11821 gtcgcgctcc atctgcattg ctttcaggtt gcgcgcttca gggctattac tgttaatgag
    11881 gggcacatct atttcatcgc cctgacgcac attgtcaaag ccccgcgcaa aggtacgaaa
    11941 ctgattcagg cgacgtaatt catcaacggt gataccaaag cgtgccgcga ccgttggtgg
    12001 cgtttccaat gcccccaggg tgtatggaat agttttaacg ttcggcagga tcagggacgt
    12061 ggcagtatcc gaatgcgcat gattaatatg cgtaggggca gcggccataa cagggaatat
    12121 agtttgtgta atcaggcaaa taccggcggt cagacgacgt aaacgatccc ccaatccaca
    12181 tatggtgtta tcgccccgac gctcgccatt cccatgcgct ttccctgcca tttgtcatcc
    12241 ctgtcgctat gcgttcagat taaatgacct ttctttaaca tgagggaaaa tgatgattca
    12301 tgattagcaa atgatgaata caggcggaat aatggaaata acaacaagat aagaacaata
    12361 cctgatcaag cgttatcagg gggcagaaca ggagagcttt cccgccagag aaataaccct
    12421 cctgtaaata cgttacttca ttttcacctt tgcggtgacc aataacgccg tcagcaacat
    12481 caatgtgccg gacaacatca acggcgaagt cagaccaata ttatccagcg cataaccgcc
    12541 gattgccgcg ccacaggtat tagcaagctg aataaccgcc acctgaatag acccggcttt
    12601 ttctgcctga tcggccagtg agcgggtgat ccacgttgac cagccgacag gaaccaatgc
    12661 aaaagttagc ccccagataa tcgccacgcc ggtagcaacg attttatcgc ttccccacaa
    12721 cgtcagcacc aacgcactca cagccagtat taacggcgcg cctgctaagg ccagttttac
    12781 cgaacgttta agaatgaacg acgaaagcga cgtaccaata aagctggcga taccaaaact
    12841 caacagcacc agagttaagc catccacgcc gaatcccgcc aggttcatat acaccgggcg
    12901 aatatacgtg aagaaagcaa actgcccggc gaaagacatg aagatggcga tcatccctgc
    12961 catcacaccc ggacgttgta ataagcggaa agtattttgt ttctgatgcg agggttcgcc
    13021 tggcagcgaa ggcaatgatt tgataatcca gaaaatacac agcacgccca tcaccgccgc
    13081 cgcattaaag acattgcgcc aaccgataag ctcgcctaaa aaactgccca acggtgcggc
    13141 aatcaccagc gcaatagaaa ccgcgccgaa gatcaccgac agcgccttcg gcaccgtacg
    13201 cggcggtacc agacgcatgg tcagcgacgc cgacatcgcc cagaacccgc ccagcgccag
    13261 ccccagacag gcacgaccaa ttaaaagcaa actgaatgag ttagcaaagg aaaccagcaa
    13321 acaggagagc gtcagcaaaa cggcaaataa aataacaacg tagcggcggt cagtggcctg
    13381 aattgtctgg gtaataaaca aactggcaaa cattgccaca aaggcggtca cggtcaccga
    13441 ttgcccggca accccttccg aaatgcctaa atcctgggcc attggcgtca acaaactgac
    13501 gggcaaaaac tcaacgataa tcagacaggc gacacaaaac gccaccgaga aaacggctga
    13561 ccagttcggt cgggtgatgg catccgcgcc gcggttttcg gcaataaatt cactcatggt
    13621 gttacccgtg gcatgtttca ggggaaaagc cgaccacttt aacattgaaa tgtgtgacgc
    13681 atttaacgtt tttgcaactt ttacggtgtt gtgggaaaag ttcgaggctg ctcgaagaaa
    13741 caaaaatcat agggcaagct gatatgcatt ttcgcgattg ggttgggaat aagggataaa
    13801 gtattgcact tggtgatata ctttaataac aggacctcaa cgatatgcgc gtgtttaaaa
    13861 ccaaatggtt tgcaagggaa gccaggtctc atgcaataac agatgaagag ttgtgccggg
    13921 ccattttaga aacggagcaa ggtaaagctg atgcacttgg tggtggcgtt tttaaaaagc
    13981 gccttcacca aaatcgtgag cgtgcaatta ttctggcaaa agggggaagc aattggtttt
    14041 acacgttttt atatgcaaaa caggatatgt cgaacatcaa tagtcaggaa ctggctgggt
    14101 ttcgtgaact tgcaaagcat tatgccttcc tgacgaaagc ccagctaaca gcaatgatta
    14161 acactaaaga actgacagag atctgctatg actgcaaaaa ctaaatttaa aagtcctgca
    14221 tttgaggcta ttcacagcgc agcggctgga ttgtccagcg tcgatgctat ttctgccgaa
    14281 acaatgcgta cttttgacaa agcctgcctg accagcgtgc aagacttaca acctgttgag
    14341 ataaaagcac tgcgggaaaa attaaaagtg agtcagcccg tcttcgctca ctatctcaat
    14401 accagcgttt caactgtaca aaaatgggaa agtggcgcta aacgccccaa tggcatttca
    14461 ttaaaactgc tcagcattgt gcaaaaacac gggctggatg tgctgctgta aatagaacgt
    14521 agataatgcg ttcacctact gtcagttccg ataccggacg cggcgtgaac gccttatcca
    14581 ctcgacaggt caggcacggt ctgtaggctg ataagacgaa gcggttttaa tgcagccagt
    14641 gaacgccctc ggcaggctga aagaatgcgt tatagcgcat ctgaaaagca ttaatatcct
    14701 gcatttcagg ttcagtttcg cgcagaaaac ccagcgccag cctgacctga gcatcggtaa
    14761 tcggcgcggc aaggcgggct ttttgtaaaa tccgatgcca ttgcagcagg ttctgttcgc
    14821 taccatcgac aatgacaatt tgccagatga gcaacacctg cgcggcgttt tgcaggtgcg
    14881 actcatccgg acgatgcaaa taacgtaaaa attcgtcacc agctgacttt cccatttgat
    14941 cgaccatcga ctccacgggt acgacatcga tattcagccg atcgctgaga cggcgaatcg
    15001 cttttctggc cccggatgtc agcacccgaa aaccaaccac aaacactacc gccagcgtgg
    15061 cgagcattat ccagatcatg agttctcctg cgtaatcaga actcatgata tctggaaacc
    15121 ctcgccagtt actaatccac cagaataatc ttcagtgcaa acagagctgc gaccaccacc
    15181 acacacaggt tcagatcgcg ccagcgcccg gtgcatactt tcatgatgca gtacgacata
    15241 aagccgagtg caatcccttc ggtgatcgag aaagtaaagg gcatcatcac cgtggtaata
    15301 aatgcgggca ctgattcggt aaaatcatcc cagttaacgc gcgccaggct cgaagtcatc
    15361 agcacgccaa caaagattaa cgctccggcg gttgcgtaag gaggaaccat cgccaccagc
    15421 ggtgagaaga acataactaa caggaacata acgccgacca cgacagccgt tagccccgtg
    15481 cgaccaccga ctgccacacc agaagtactt tcaatatagg cagtaacaga cgaggtaccg
    15541 ataaacgcgc ccgccaccga actgacgcta tcaacataca gcgccttatt catattgggg
    15601 aatttaccgt tactatctat taagccagct ttatcagtta caccaattaa tgttcctgat
    15661 gaatcaaata ggttgatcag cataaaggag aaaatgatac cggcgagttc aagtgttaac
    15721 gcgccgctca aatctacttc accaatgacg ccgctaatat caggcggaat ggaatagacg
    15781 ccgctaaaat gaacatcacc gaaaaataat ccacagcaag acgtcaccac aatagaaacc
    15841 agcaccgcgg catgaaaatg acgtgatgac aacacggtta taataaaaaa ccctaaaata
    15901 cctaacaaca caccgtgaga acttaaatcg ccaatcatca ccagggtgtc tttattggcg
    15961 acaataacgc cagtattttt taatcccatt aaggcgataa ataatccaat tccactggtg
    16021 ataccaatac gtaaacttaa tggaatgttg gagatcatcc agtaccggat acgaaacagc
    16081 gtgagcaaaa atagcccaat tgcaccccag aatatcgcgc ccatcccggt ttgccaggag
    16141 atccccatag cccccacgac cacgaaggca aagaaggcgt tcagccccat tgccggagcc
    16201 agcgccacgg gtaagttagc aaatatcccc atcgcaatac tgccgatacc ggcaatcaaa
    16261 caggtggtaa caaacaccac tttcgggtcc atttgtgccg cgccgaggat ttgcgggttc
    16321 acaaaaacga tgtacaccat ggttaaaaaa gtggtcatcc ccgcaatcaa ttctgtgcgg
    16381 acggtggtcc catgctgagg tagtttaaat aatcgcgaaa gcgtccctga ttcattactc
    16441 acgtaatcgg tattgtcatt attcattttt ttgtccattg gaggagattt aatcccttgc
    16501 ttttgtaaaa caaatgacaa caacggttca gtgataatta tcacatttca attgcacatt
    16561 aatggatatt ctttaataat ctcgcgacgt ttctttatga taaataataa tcaaattgat
    16621 aaaatcaaaa tgagaaaaat atgaataatt ctattaacca taaatttcat cacattagcc
    16681 gggctgaata ccaggaattg ttagccgttt cccgtggcga cgctgttgcc gattatatta
    16741 ttgataatgt ctctattctc gacctgatca atggcggaga aatttccggc ccaattgtga
    16801 ttaaaggacg ttacattgcc ggtgttggcg cagaatacac tgatgctccg gctttgcagc
    16861 ggattgatgc ccgcggcgca acggcagtgc cagggtttat tgatgctcac ctgcatattg
    16921 aatccagcat gatgacgccg gtcacctttg aaaccgctac cctgccgcgc ggcctgacga
    16981 ccgttatttg cgaccctcat gaaatcgtca acgtgatggg cgaagccgga ttcgcctggt
    17041 ttgcccgctg tgccaaacag gcaaggcaaa accagtactt acaggtcagc tcttgcgtgc
    17101 ccgctctgga aggctgcgat gttaacggtg ccagttttac ccttgaacag atgctcgcct
    17161 ggcgggacca tccgcaggtt accggccttg cagaaatgat ggactaccct ggcgtaatta
    17221 gcgggcagaa tgcgctgctc gataaactgg atgcatttcg ccacctgacg ctggacggtc
    17281 actgcccggg tttgggtggt aaagaactca acgcctatat tgccgcgggt attgaaaact
    17341 gccacgaaag ttatcagctg gaagaaggac gccgaaaatt acaactcggc atgtcgttga
    17401 tgatccgcga agggtccgct gcccgcaatc tcaacgcact ggcaccgttg atcaacgaat
    17461 ttaacagccc gcaatgcatg ctctgtaccg atgaccgtaa cccgtgggaa atcgctcatg
    17521 aagggcatat cgatgcctta attcgccgcc tgatcgaaca acacaatgtg ccgctgcatg
    17581 tggcatatcg cgtcgccagc tggtcgacgg cgcgccactt tggtctgaat cacctgggct
    17641 tactggcacc cggtaagcag gctgatatcg tcctgttgag cgatgcgcgt aaggtcacgg
    17701 tgcagcaggt actggtgaaa ggcgaaccga ttgatgcgca aaccttacag gcggaagagt
    17761 cggcgagact ggcacaatcc gctccgccgt atggcaatac cattgcccgc cagccagttt
    17821 ccgccagcga ctttgccctg caattcatgc ccggaaaacg ctatcgggtc attgacgtca
    17881 tccataacga attgattacg cactcccgct ccagcgtcta cagcgaaaat ggttttgatc
    17941 gcgatgatgt gtgctttatt gccgtacttg agcgttacgg gcaacgactg gctccggctt
    18001 gtggtttgct cggcggcttt ggcctgaatg aaggtgcgct ggctgcgacg gtcagtcatg
    18061 acagccataa tattgtggtg atcggtcgca gtgccgaaga gatggcgctg gcggtcaatc
    18121 aggtgattca ggatggcggc gggttgtgcg tggtacgtaa cggccaggta caaagtcatc
    18181 taccgttgcc cattgccggg ctgatgagca ccgacacggc gcagtcgctg gcggaacaaa
    18241 ttgacgcctt gaaagccgcc gcccgtgaat gcggtccgtt acccgatgag ccgtttattc
    18301 agatggcgtt tctttctctg ccagtgatcc ccgcgctgaa actaaccagt caggggctat
    18361 ttgatggcga gaaatttgcc ttcactacgc tggaagtcac ggaataaaaa aaaaaaagcc
    18421 cggcgtcatg ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt
    18481 ttttctcgcg gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac
    18541 aaccaatcgc ggcgatatcc agcgcggcga aggtgcctgc ccagccggta aggccgaata
    18601 ccggcgtccc atcggcaatc attcccagac ctaacttggc aaagctgtcg ccaatcaggt
    18661 aagcaaaggt gcctttaata ccatcggcag caccaatcgc ttttttaggt acaaaaccaa
    18721 cagcagccac accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag
    18781 aagccagata gatatattcg ttgctggcat gttgatacac accgagcgtg gcgataatca
    18841 gcgccagcgc gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc
    18901 cccacagcag cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca
    18961 ccgctttaga gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa
    19021 tacgtaccac atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca
    19081 gcacatactc aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttatcct
    19141 cttcgctgat ctcctcgccg aacagttctt cagctttgcc gaggccataa gattccgggg
    19201 agtcgctgcc gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga
    19261 taaacatgcc gatgacatgg ccatcgaaca ggtaatttgc gccgaacagc gccacacctg
    19321 ctgcgcctgc accgccaagg ttgtgagata tgttccagaa accgaggaag gtcccgcgct
    19381 tacgacgcgg cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtgctctgga
    19441 aaaagccgct taaggcgtag aaggcaatca tcagaaacag gctaaccgag ccgctgccca
    19501 tactggcact gaagcccagc atacaaatgg cagacaggat aagcatgaac ggcaggaatt
    19561 gtttggtgtt tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga
    19621 tggagaaacc caggccgatc atccccaact gcgtcatgct caacccgtag gtcgagatca
    19681 tgtcgttctg cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa
    19741 agaccaccag gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg
    19801 gaaggtccag ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa
    19861 atgaatacct gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc
    19921 gtccaggcaa aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat
    19981 gcatcacgct tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat
    20041 cccggcggcg atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt
    20101 cgccagtggc caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt
    20161 cgccgcccct gccgcctctt tgtgggaaca ctctgccgcc gccatgccga ttaacatctg
    20221 cgggccaaag acaaaaaaac caatagtgaa gaagcaggtt gcctgcatca cgtagctggc
    20281 aaatggcatc agccacagcg agccgactga aagcaaaatt ccggcggcga aaatcaaatt
    20341 catcggccca cggttgccgt taaacaattt gtccgagccc caaccggcta ccagcgcacc
    20401 gataaatccg cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc
    20461 cagcgtctcg gacatataca aattgcccca gtcgttgatt gccgcccgga ccacatagac
    20521 cagcacatag caaaacgaga gcagccagat atacggattc agtaacacat atttggtgag
    20581 gatctcttta cgcgtcagcc ctgccccttc ttgttgttga gcaatttcca gtgcgtcgtg
    20641 ccgccattca ccgaccgctg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca
    20701 gagaaaaatc cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc
    20761 gtaatgtagt gcagccgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc
    20821 cgtgttccat aatgcccacc aaccgccgcg ctcggtacga gaataccagg cggttaacag
    20881 acgcgcacac accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagtacggc
    20941 aaacgcccat agcgacgtcg agaagccaaa cagaatgttg ataatgcccg tggcgataag
    21001 ccctatcccc ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt
    21061 cgacacgccg taagtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag
    21121 cacgccgtta gcaaggattt ctggtaccgc ggcgttaaaa cttttccgcg tgaagtaaaa
    21181 cagcgcgtaa cccagccaga tggtcagcag aatatgccga cgccagtagc gataacgggc
    21241 atcaatttca tgtttatcag tcattaatgg cgcatcggca ggcgctttca gaaacggcaa
    21301 catcatcgaa ccttagacat aacgttgagg tagagaaacg ctgacacgcg tgccgtgcag
    21361 acaggaaatg tgtaatgtgc cgcccagcgc cgttacgcgc tcgcgcattc cggtgaggcc
    21421 aaaaccttgt tgcccggaac ccggtggcaa accgctacca tcgtcttcaa tcaccagcat
    21481 caaccgttca tcctgctgcc agccttgcag cgtaaccgcg ctggcatcag catgtttcac
    21541 aatgttgttc agcccttcct ggcagacgcg aaacagcgtc acgcgctggt tttcacttaa
    21601 cgctgactca tcgatacgcc attcgagatg gctgacaatg ccgcgccctt ccagctccat
    21661 ttcccgcatc agtgagcgga tggcctgctc cagggtgaga tcatccagct ggcgcggacg
    21721 caaccgccct aacaaacggc gcaccgcatc gtaaacaccc agcgacagtt gttcgatgag
    21781 ctgcccgctc tgcttcacgc tggcgttatc tgccgccagt cgctgaacaa tgcccgcctg
    21841 agtacgaata gcagtgatgg tctgaccgat atcgtcgtgc agctcacgcg ccacgtcgcg
    21901 gcgcacgctc tcttcagttt ctaacaaacg ttcagccaga tgctgattgc gcgctagttc
    21961 tttttgcagc gactggttaa gttcacgcaa ccgctggata cctgcgccca gtaacaaccc
    22021 tgtcagactt tgcaccagca gcgagagcaa taagtccacc ggatgatcgc gccaggtttg
    22081 actggcgatc agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccagccata
    22141 gtgccaggcc agcgcgataa tcggcagcgc caggcagaat ggtgtaaagc gcgacagttc
    22201 gtccggcaat cccaactgga gccagagact aataacaaac agcagcaagt accagaccag
    22261 atgccgcccg cgccagttga tcggttgaga aactaacgac gggccgagcg gcagccaggt
    22321 gttattggcg agatagtgcc agaacaccag acatataggg gccagcgtca ggccgccagt
    22381 taaagtcagt agtagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg
    22441 caacaacgcc gccgccgtca gggccgcccc ctgtaacagc aaggtgcgcc agtcacgttg
    22501 atggcgatag cgcgagatca gcgcaacggg tagtaacgtc agtaaactac cgatcatcaa
    22561 taaggaaaaa tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc
    22621 gcccagcagc accggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc
    22681 aaacggaaat aacagcaccg ccatatcagg gcgctcaacc aggtgcaggc tgatactcca
    22741 caagcaaaac catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga
    22801 gaacaacgtc ttcatcacca gccatcaaac atgcggcgcg ccagttcaac gtcgttactg
    22861 acgcccagtt tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc
    22921 agttcggcgg caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt
    22981 tcgcgtttgg ttagcgggtc ctggcgaccg gatgccagtt taatggcaat atccggcgtc
    23041 agataacagc cgcccgtggc aaccgtatgc accgcagcaa tcagttcgtc agggctacaa
    23101 cgtttggaga gaaagccgcg cgcccccgcg ttaagcgcct gctcaaccag cgcaggactg
    23161 tcgtgaacgg agagcataat tgtcgccata cctttcggca gctggcttag caactccaga
    23221 ccggagatat cgggcatgga gatatcgcaa atacacacct gcacaccacg ccccggcagc
    23281 cccgccagcg cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc
    23341 cccagcaact gcgcaaagcc ggagcggacg ataaggtgat cgtctataag ggcaacggtg
    23401 atcatgattg ggtcctggcg gataaaaaac gcgcatacct taacgataag cgcgatgttg
    23461 ttcaagttgg atatggggta tgtattggct taatgatgtt gcgcttatcg tcaaaacgct
    23521 tacctgagag gtaagcgttt ggtgcgtgtt attgagaaaa agcaaattat ttgtaggttg
    23581 caatcgtaat attgcctgag aaatttctcc ttagattgcg gatatcatta tcccttgacc
    23641 aataatcatc tttgtgtcta taactttcat cggaatgtac atagggagaa tcatcttcag
    23701 cgtcgttatc gataatattt tcgcccttag tggtatagat ataattagca acatcaaagg
    23761 tgtcattcgc gttatttacg actgctccta acatgcaaag ggtttgtgtg aatccgtatt
    23821 ctgagaaatc gacatgctga tggtaacttc taccattcaa agcaaccaaa ctcggacaaa
    23881 catctattgt atcgccaaca gatgtagcgg cgcgacgcac atcaagtgca gcgttatcat
    23941 tagcaacatg aaatgcttgt gaacaggcat aaaacaaatt tccaatcccg gtacgactgg
    24001 ataaacaaga aataattttt gctatttttt ctggctttat accatcctgc actaaagaat
    24061 ctctcaccag atgacattca tcaggtgtga aaacagcttt cttgaatatt tcctcataaa
    24121 cttcataaga aggagattca gagagagtga gagatttgat ggaatgtgtt gaatctgtaa
    24181 tttttttgcg aagtaattca cttatagtgc cgtttatttt ttcaccacaa cataaaacaa
    24241 atgaacttcc ctccagaact tgtctttctg tatctgcaag gag
//
LOCUS       DAZUCE010000058        24179 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZUCE010000058 DAZUCE010000000
VERSION     DAZUCE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.058"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(92..1219)
                     /gene="rlmC"
                     /locus_tag="ACHY03_001941"
     CDS             complement(92..1219)
                     /gene="rlmC"
                     /locus_tag="ACHY03_001941"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFS3905176.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVVEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWVRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKI"
     gene            complement(1260..1748)
                     /gene="ybjO"
                     /locus_tag="ACHY03_001942"
     CDS             complement(1260..1748)
                     /gene="ybjO"
                     /locus_tag="ACHY03_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFS3905177.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQV
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFFPAASRRFFQ
                     LQ"
     gene            complement(1808..2653)
                     /gene="potI"
                     /locus_tag="ACHY03_001943"
     CDS             complement(1808..2653)
                     /gene="potI"
                     /locus_tag="ACHY03_001943"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFS3905178.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(2650..3603)
                     /gene="potH"
                     /locus_tag="ACHY03_001944"
     CDS             complement(2650..3603)
                     /gene="potH"
                     /locus_tag="ACHY03_001944"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFS3905179.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGCKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNFGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALTRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIVMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(3613..4746)
                     /gene="potG"
                     /locus_tag="ACHY03_001945"
     CDS             complement(3613..4746)
                     /gene="potG"
                     /locus_tag="ACHY03_001945"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFS3905180.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(4841..5953)
                     /gene="potF"
                     /locus_tag="ACHY03_001946"
     CDS             complement(4841..5953)
                     /gene="potF"
                     /locus_tag="ACHY03_001946"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFS3905181.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELQKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQAPKIDRVRTRAWTKVKSGK"
     gene            complement(6304..6780)
                     /gene="ybjN"
                     /locus_tag="ACHY03_001947"
     CDS             complement(6304..6780)
                     /gene="ybjN"
                     /locus_tag="ACHY03_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFS3905182.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(6868..7770)
                     /gene="rimK"
                     /locus_tag="ACHY03_001948"
     CDS             complement(6868..7770)
                     /gene="rimK"
                     /locus_tag="ACHY03_001948"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFS3905183.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(7831..8553)
                     /gene="nfsA"
                     /locus_tag="ACHY03_001949"
     CDS             complement(7831..8553)
                     /gene="nfsA"
                     /locus_tag="ACHY03_001949"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFS3905184.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(8537..8824)
                     /gene="ybjC"
                     /locus_tag="ACHY03_001950"
     CDS             complement(8537..8824)
                     /gene="ybjC"
                     /locus_tag="ACHY03_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFS3905185.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            8984..9241
                     /gene="grxA"
                     /locus_tag="ACHY03_001951"
     CDS             8984..9241
                     /gene="grxA"
                     /locus_tag="ACHY03_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFS3905186.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(9271..9648)
                     /gene="ybjM"
                     /locus_tag="ACHY03_001952"
     CDS             complement(9271..9648)
                     /gene="ybjM"
                     /locus_tag="ACHY03_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFS3905187.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            9918..11603
                     /gene="ybjL"
                     /locus_tag="ACHY03_001953"
     CDS             9918..11603
                     /gene="ybjL"
                     /locus_tag="ACHY03_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFS3905188.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            11936..12487
                     /locus_tag="ACHY03_001954"
     CDS             11936..12487
                     /locus_tag="ACHY03_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFS3905189.1"
                     /translation="MDINELKECLHLEVIGKSRKFTWRKVIVRAMKHRRVRYLFWWRI
                     AKYGHEKGGYWRKIAGKIERKILDSYDVKIPLVVDIGKGLDISYLTGVVIGHNVKIGE
                     NCSIKPGVTIGLRGHFDEMDIQIGNNVTIGCNASILGGKVYIGDNVTIGAHALVLHDI
                     PENSIFINKIEYEIIPKKVIAEM"
     gene            complement(12547..13083)
                     /gene="rcdA"
                     /locus_tag="ACHY03_001955"
     CDS             complement(12547..13083)
                     /gene="rcdA"
                     /locus_tag="ACHY03_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFS3905190.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13167..14375
                     /gene="ybjJ"
                     /locus_tag="ACHY03_001956"
     CDS             13167..14375
                     /gene="ybjJ"
                     /locus_tag="ACHY03_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3905191.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSALLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVERKMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKSVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14375..15190
                     /gene="ybjI"
                     /locus_tag="ACHY03_001957"
     CDS             14375..15190
                     /gene="ybjI"
                     /locus_tag="ACHY03_001957"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFS3905192.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKMVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15276..15560
                     /gene="ybjH"
                     /locus_tag="ACHY03_001958"
     CDS             15276..15560
                     /gene="ybjH"
                     /locus_tag="ACHY03_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFS3905193.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15601..16833)
                     /gene="mdfA"
                     /locus_tag="ACHY03_001959"
     CDS             complement(15601..16833)
                     /gene="mdfA"
                     /locus_tag="ACHY03_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFS3905194.1"
                     /translation="MHNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLANGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17118..17714
                     /gene="ybjG"
                     /locus_tag="ACHY03_001960"
     CDS             17118..17714
                     /gene="ybjG"
                     /locus_tag="ACHY03_001960"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFS3905195.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPNDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17772..18530
                     /gene="deoR"
                     /locus_tag="ACHY03_001961"
     CDS             17772..18530
                     /gene="deoR"
                     /locus_tag="ACHY03_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFS3905196.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18577..19779)
                     /gene="dacC"
                     /locus_tag="ACHY03_001962"
     CDS             complement(18577..19779)
                     /gene="dacC"
                     /locus_tag="ACHY03_001962"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFS3905197.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20026..20652
                     /gene="gstB"
                     /locus_tag="ACHY03_001963"
     CDS             20026..20652
                     /gene="gstB"
                     /locus_tag="ACHY03_001963"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFS3905198.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20649..21764)
                     /gene="yliI"
                     /locus_tag="ACHY03_001964"
     CDS             complement(20649..21764)
                     /gene="yliI"
                     /locus_tag="ACHY03_001964"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFS3905199.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFSTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21875..22258)
                     /gene="bssR"
                     /locus_tag="ACHY03_001965"
     CDS             complement(21875..22258)
                     /gene="bssR"
                     /locus_tag="ACHY03_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFS3905200.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22471..23796
                     /gene="rimO"
                     /locus_tag="ACHY03_001966"
     CDS             22471..23796
                     /gene="rimO"
                     /locus_tag="ACHY03_001966"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFS3905201.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLKITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            24022..>24179
                     /locus_tag="ACHY03_001967"
     CDS             24022..>24179
                     /locus_tag="ACHY03_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3905202.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQG"
BASE COUNT         6096 a         6263 c         5934 g         5886 t
ORIGIN      
        1 accgcgatcg atcggttatt attaatcgat cgatcacgat tattattttg agtgaatagt
       61 tgttgccgaa aaatttactg ccgtgatgta tttaaatttt taccagcagc gtcaacactt
      121 cataatgcgc ggtgtgaggg aacatatcga aaagctgcac ccgttcaatg cgataaccag
      181 gcagttctcg gatatctttc gccatagttt gggcgttaca gctggagtag ataataaaac
      241 gcggtgccat cgttgagaga taatcacaca gcggtttacc aatgccgcgg cgcggcgggt
      301 taaccagcac cagctccggc acctccccct gagcggtggc aaactgagtg gagtccagcg
      361 cctgaaattg caaacgcgtt aagcccagtt cagcggctga ctgctttgca caggcaatgg
      421 cctctggcgc aatttcgatc ccggttaact gcatgtcagg cgtcgcgcag tgtaaaccaa
      481 agccccccac accgcagaac agatcccaca tatgtttaac cggcagctgt cgtacccagt
      541 cgcgcgcggt agcgtacagc tggctggcga ccgccggatt agtctggaag aaactttgcg
      601 gacggaccca cagcggcaca tcgttaaaac gctccgccag tgcctgttgt tcggtcaggt
      661 agatctccgt ctccccttcc ataatcgcca tatgtaccgg ctgaatattg acggtaataa
      721 cttttagctg cggtagttgt tcctgtaacc acggtagcgc cttacgcagt tgcgccagtt
      781 tggtttcgga acgcaacaca aaacgcagca tcatgccgcc atcgctctgg ctttcagtca
      841 gcaaaatgta tttcagttcg ccacgcttac gcgccacgtt ataaggcgtt agccccgcac
      901 gggcgataaa cggttttagc gccgcaaaaa cgggcgcaaa tgaggcagga taaagcgggc
      961 agtcacaaag gtcttccggt gtgccgtctc gatgcagcat accgagcagt ggtttttcaa
     1021 cgctaccact caccaccatt ttggctttat tacgaaaccc ttgctccggg ccagacaccg
     1081 gcgcgcacca ttccacgacc ggaaaatcgg cgagcagatt tttaagatcg gcggttttag
     1141 cggagagttg ctctggaatc ggctgcgtta tccactgaca ggaacgacag cgacccgcgt
     1201 cgtaaagtgc gcactgcata cattgacctt cacatcatca ggggcgacga ttatacacat
     1261 tattgcaact ggaagaaccg ccgactggcg gcgggaaaga acagcagcat caggataagc
     1321 atatccggca gcttctgcag catcaggcta tggaagattt cacgtttcga ttcaccggga
     1381 atactgaaca gctccggata accataaccg agcgaggccg cccacagata actggcggcc
     1441 gtgacttgag tcagcagata cagccagcgc gcccagcgac gccctttcac cagtgaaaac
     1501 gcacaccaga tctcaataaa aaccagcacc agactgctta aaaagaccag cgttaaactc
     1561 caggtttgta cgctgcgatg aatgaattcg ccaataccgc gcacgcccag cgtattgaaa
     1621 atcatcagca cgtcgaggcc acggatcata ataatggcga gcgccgccac ctgcaccagc
     1681 gcaggcacat tcaggcgagc atgagatgaa gatgttttct taaaaaatcc caacgtttcg
     1741 tcttccatga aaacaatgcc gcgacatgcg cggcattatg tagccaggtt ggcaaatttt
     1801 agtgtcttca gccacgtctt gcacgctgga tgtcgcgtat ccgctgtttt tctgcgcgag
     1861 ccatcagata ccaggcgata aatccgacaa ttccgaccgc accaaggatc aatgttgcca
     1921 gggcgttgat ttccggattc acccccatcc gcacgctgga aaagaccagc atcggtaacg
     1981 tggtggctcc cggcccggaa acaaagctgg cgatcaccag atcatcaagc gacaaagtaa
     2041 aagccagtaa ccagccagaa atgatcgcgg gcatgatcat cggtagcgta atgacgaaaa
     2101 acactttcag cggcgtcgca ccgagatcca tcgctgcttc ttctatcgag cgatccagtt
     2161 cccgcagacg cgacgaaata acgactgcca cataagccgt acagaaagtg acatgcgcca
     2221 gccagatggt aagcataccg cggtccgcag gccagccaat ggcatgagca agcgcgacga
     2281 ataataacaa cagcgacaag cccgtgatga catctggcat taccagcggc gcggtgatca
     2341 taaaggcaaa gccatttgat ccgcgaaacc tgccaaagcg caccagcacc accgccgcaa
     2401 tagtcccgag gatcgccgcc gccgttgccg cacaggcagc aattgtcagg cttaaaccaa
     2461 ccgcactcat catcgcgtca tcgcgcaata actcaccata ccagcgcgtt gaccagccgg
     2521 cccacaccgt caccagcttc gagctgttaa acgaatagat aaccagcatc agcattggcg
     2581 cgtagagaaa ggtgaaaccc aacagcaaaa tcacaattcg ccagggcgaa cgaaccaccg
     2641 gtaaattatt catccgtgtt ctcccacgct tttttgctga tgtttgtgga accacattat
     2701 cggcacgatt agcagcaaca acatgacaat cgccactgcc gaggctactg gccagtcacg
     2761 gttattgaag aactcctgcc acagtacgcg gccaatcatg atgctatccg gaccgccgag
     2821 cagttccggg atcacaaact cgcctaccgc aggaataaag accaacatcg aaccggcaat
     2881 aattccgcct ttggtaagtg gcacaataac agtaaagaac gttttcaacg gtcgtgcgcc
     2941 gagatccagt gccgcttcca ccagcgaata atcaatacgc gtcaacgcgg tgtagattgg
     3001 cagcaccata aacggcacat aggcgtaaac aatgccaata tagaccgcca gattggtatg
     3061 cagaatggtc aaaggttgat cgataacccc cagccacagc agaaaattat tcagcacacc
     3121 gttgtttttt aatattccca tccaggcgta gacacgaatc agaaacgagg tccacgacgg
     3181 caggatcacc agtagtaata agatattacg ggtcgaaggc ttactgtgcg ccaccgccca
     3241 cgccagcgga tagccgatca gtaaacagca aaatgtcgaa atcgccgcca cctgtaacga
     3301 ctggagataa gcatcgaaat agagcggatc gtcggtcagt tgcaggaaat taccgaaatt
     3361 aagggtgatg gacagttgcc cgtcagccca ctccatcagt tcggtataag gtggaatagc
     3421 gcgcgccatc tccgccaggc ttattttaaa gacaatcaga aatggcagca gaaacagcaa
     3481 gatcaaccag atatacggca acgcaatgac cagtttgcac ccatgcttca tttgcaactg
     3541 cgacaaccac agcttaaatc cgcccggcgg tttcgactgg gcagcaggtt caagtgtact
     3601 catctttgct ccttaaaccg tcagcaccac acagctgtcc acttcccagc acaaacgcac
     3661 ttcgtcgccc caggtcggta accctttacg atgacgatgg gcgttttgta gctgggcgct
     3721 aatcatctgc ccacttttca gacgaacgtg atacaccgaa agatcgccga gataggcaat
     3781 gtgtatcacc tcccccaccg cgaagttaca accattggcg ggcggctctt cgcaaagcat
     3841 gattttttcc ggacgcagcg ctacgtgtac cggcacgtta tcgaccactg aagcatccgc
     3901 atcgactttc agtggatgca ccagccccgg cgaataaagc accagaccat cttcctgacg
     3961 ctctttgagt acaccttcaa agacattaac cgagccaata aattcggcgc tatagcgggt
     4021 agtcggatgc tcgtagatct cttccggttc gccaatctgg acaaatttcc cacgattcat
     4081 aatggcgata cgccccgcca tggtcatcgc ctcttcctga tcgtgggtga ccatcacaca
     4141 agtcacaccg acgcgctcca gaatatccac cacttcaagc tgcatccggt cacgcagctt
     4201 tttatccagc gcgcccatcg gctcgtcgag cagtaatagt ttcgggcgct tggcaaggct
     4261 tcgggccagt gccacacgct gtcgctgacc accggaaagc tgatgcggtt tgcgtttggc
     4321 gaactcctgc atatgcacaa ggccgagcat ctcattgacc cggctggcaa tttccgcttt
     4381 cggtagtttg tcctgtttca ggccaaaagc gatgttctgt tccacggtca tatgcggaaa
     4441 cagcgcgtaa gactgaaaca tcatattgat ggggcgcagg taaggcggca cctgtgacaa
     4501 atcgacgcca tcaagcataa tctgcccggc tgaaggttgt tcgaaacctg ccagcatacg
     4561 cagcagcgtg gacttgccac agccggatgc gcccagcagc gcaaagattt cacctttgta
     4621 gatggtcagg ctgacatcat caaccgcatg ctgaccatcg taggatttgg tcagattgcg
     4681 gatttctaat agcggggtca gcgccttacg ggttttcgcc tgggggcgag ggatagcgtc
     4741 attcacggct gctctccggc attaaatgtg tgcaagcgca ccatgttggt gcgaattgcc
     4801 ggagggagtg gcgctcccgc ccggcatcta aggctgcgga ttattttccg ctcttcactt
     4861 tggtccacgc gcgggtgcgc acacggtcga ttttcggagc ctgtactttc agagtgaaca
     4921 acttcgcgcg gacatccgca ggcggataaa tacccgggtt atcacggact tccgcactca
     4981 ccagcggcgt ggctgcttta ttggcgttag catagaacac atggtcagag atatgtgcca
     5041 ccacatcagg acgcagcagg taattcagga actgataggc ttcgtcttta tttttggcat
     5101 ccgcaggcat ggcgaataca tcaaagaacg ccatcgcccc ttcttttgga atcgagaacg
     5161 agacattcac gccattcttc gcttccttcg cgcggtttga cgcctgccag acatcacctg
     5221 cccagccgat agcgacgcaa atatcgccgt ttgccaggtc gttaatatat tgagatgaat
     5281 ggaaataacg aatgttcggg cgcagcttta acagcagatc ggtagccggt ccggtgtaat
     5341 catccgcttt agtgctgttg ggatctttgc cgagataatt cagcacggta gcaaaaactt
     5401 cttctggcgc atcgaggaaa gatacaccgc agcttttcag tttttccaga ttttcaggct
     5461 tgaggatcaa gtcccagcta tcgacgggcg cgttttcgcc cagcaccgct ttgactttat
     5521 cgacgttata gccaatcccg gtcgtcgccc acatataggg catagcaaat ttattgtcgg
     5581 gatcgtgttt ggcgaccaac ttctgcagtt ccgggtcgag attcttccac tccggcaatt
     5641 tgcttttatc cagcggctgg aaaactcccg ctgtcagctg gcgttcaaga aagcttgctg
     5701 acggcaccac caggtcaaag ccggtgctcc cggccattaa tttgccttcc agtacttcgt
     5761 tagagtcgaa aacatcgtag acgactttaa taccggtttc tttttcaaag ttagccaccg
     5821 tgtcgggggc gatataatca gaccagttat aaatgtggag tgttttttgt tcagccgcga
     5881 gcgtgccgac agagacggcc atcagagcac ccgcaaccag acccgaaagc cattttttat
     5941 ttaaggcggt catatctctt tccttctgaa agttcgttaa caaacgaaca aaagttgtca
     6001 cactgaaaga tatgcaataa acgtgcatat tttttatcgg cgcggtgtaa gaaaagagaa
     6061 ctctctcccg gccagcactg ctcgctatat aaaagcatcg caagaataac tttagccagg
     6121 gtttgagact atagctcgga ttaaaaaacg gtatttaaca aatttttatg cgtatttgct
     6181 ctatgtgata aagccaaatc gacacaagag aagtgaaata atctttaaga aaaggttaaa
     6241 aacagcagat aattttgcag cgcgcagcca ctgcaccagc gactgcgtgc tcgaaaggag
     6301 ttatcagtgg aggaaatagt tttcggtaac gttgttttgc ccttcatcgt cagtcggtaa
     6361 cagcagttga tgggcattag cttcaagaat gaccatcgaa atctgctctt cgctctgacg
     6421 cacgaaccat gcaaactgct cataggttac gccctgcata acggataaag actggcaaac
     6481 caccagcttt ggcagattat catcctgcat atcaagaaat gctttcacgg tcagcgaact
     6541 ggcattgatg gcagataaat ccgccgccag cggcaatacg gctgaaggtc ggacttccgc
     6601 catggcagaa aacaggatcg tgttatcgat cagatcgatt ttggcatcaa agacaccgtc
     6661 gaaattctgc atatggggca gatgcagagc ctgacagtta tcacattcaa aaaaactcat
     6721 ccccaggtca tcgagccatt gacgcagcgt atccagacca ggaacgacca gcgatgtcat
     6781 aattttgtac aacctctttc gataaaaaga ccggcacagc ttacgcaaaa agcgcaggca
     6841 aaaccatgat cagtaatgtg attgcgacta accacccgtt ttcaggcaat attctgtcgt
     6901 agcgtggcgt tcgatccagc ggatcatttt acccgcgatg tcgataccgg tggttttttc
     6961 tattccttcc agccccggcg acgcattcac ttccatcacc aacggcccgc gattagcacg
     7021 cagaatatcc acaccagcaa cgtccagcgc catcgttcgc gcggctttta tcgcgatttc
     7081 acgctcctgt ggtgtgatac tggcgacgct tgccgcgccg ccacgatgca aattggaacg
     7141 aaaatcgccc tctttcgccc gccgttcaat cgcagcgacc acttcatcgc caacaaccag
     7201 acagcggata tcgcaccctt gcgcctcttt gatatattcc tgcaccagaa tatgcgcgtt
     7261 cagaccgcgg aaagcgtcaa tcacgctttc cgccgcctga cgcgtctccg ccagcacgac
     7321 gccaattccc tgcgtgcctt caaccagctt gaccaccagc ggcgcaccac cgaccatgtc
     7381 gattaaatcg ctggtatcat ccggcgaatg cgcaatgccc gtgacaggca ggtcgatgcc
     7441 ctgacgcgcc agcagttgca tggaacgcaa tttgtcacgc gcccgggcaa tggcgaccga
     7501 ctcattgagc ggatagctcc ccagcatctc gaactggcgc agtgccgccg tcccataaaa
     7561 ggtaatggcg gtgccaatac gcgggatcac tgcgtcaaaa tggggtaact tgcggccttt
     7621 gtagtgaata gaagacgccg caggatttat gttcatgtag caagaaagcg gatcaagaat
     7681 ttcaaccagg tgaccgcgct gtatagcggc ttcacgcagc cgcttacacg aatagagcgt
     7741 tccatcccgg gacaatatgg caattttcac cctgcacctc tctgtcagac ctggtcaaaa
     7801 gcccgcgtat catacatcgg cgtggcggtt ttagcgcgtc gcccaaccct gtttgtgcaa
     7861 ataatccaga ataaatgggc ggctttcttt aatgattgtt cggcggatat gatcgctcca
     7921 ggtatcccgg cgattattgc tgccacgggt gaggtaatat tcagccagtt gttcgtcata
     7981 ctgcgccagt gcgtctttat ccagcggttg atagctgttt tcatgcacca aaatggagga
     8041 cggtaaacgc ggcttaagat ccggattatc cgcaggccag ccaaggcaca gcccaaacag
     8101 cggcagaaca tgctgcggta atttcagcag ttccgtcacc gcttcaatat tattgcgcag
     8161 gccgccgata tataccccgc ccaatcccag cgattccgct gcggttaatg cattctgcgc
     8221 catcattgcc gtatcaacga caccgagcag caattgttcc gccaggccaa gctgagcatc
     8281 cggacagatc tgtaaatggc ggttaaagtc ggcacagaac acccagaact ccgccgcttg
     8341 cgctacgtgt ttttgcccgc cggtcagcgt caccagttct tcacgtaacg ctttgtcggt
     8401 aatgcgaata atgctactgc actgcaaaaa actggaactg gacgtcgcac gggcgctgtt
     8461 aataatcgcc tcacgctgcg cctcggaaat gggttcatca gtgaaatggc gaatggagcg
     8521 atggccacaa ataagttcaa tggttggcgt cattatcttt ttctctttct gaacgtgaat
     8581 attgcggtgg acggttcatc agctgtgggg caagacgttt tgccacctga agaataacca
     8641 ccaccgcagc gggaagcatg agcaaaacac cgagaaaaat catcagaatc tgcacttctg
     8701 gccgagaaaa tggctcaggc agcgacaggg agtcgcttac cgacagcagc gccaccgcca
     8761 gcaacatcat tccgataaat tccagtatca acacgctttt aggcaattta ccgatcgcgc
     8821 gcatacgctt ccctctgcaa agtgagcctt cagtctaaaa cttttcactg tattgtgttt
     8881 aacagttata gcttttagca attaatgcaa caggttaaac ctactttcag cgaatacatt
     8941 ttagcgtgat cattacaggc ataaatctat gaggagagaa ataatgcaaa ccgttatttt
     9001 tggtcgcccg ggttgccctt actgtgtgcg tgcaaaagat ctggctgaga aattgagcaa
     9061 tgaacgcgat gattttcagt atcagtatgt agatattcgt gcggaaggga tcactaaaga
     9121 agatctacaa caaaaggcag gtaaacccgt agaaaccgtg ccgcagattt ttgtcgatca
     9181 gcaacatatc ggcggctata ccgattttgc tgcatgggtg aaagagaatc tggacgcctg
     9241 atcgtctgac aagccctcgc gttgagggct ttactgattt tttctgtgct gtggtttaaa
     9301 caagctactg ataaataaga aacacagtgc ccccagcgca caccagaaca ccgcgcttag
     9361 taaccatgcc agctcttgcc agaatgagcg ggtcggtgaa aaaaacagcc gcataataag
     9421 cattgaacag ggtgccgcca gcattgcgcc aaatagaggt ttcaggactt ctctacgctg
     9481 tgaaaagaaa ctggcaactg ctccaggaag aatgaaaaac agcaagccga tttcaggatg
     9541 cccggcagcc cgaaaagcgc ctttcatgtg cgtcgccaga aaaaggcaca ccacaatgaa
     9601 gaggacaaaa cagcagattg cccccgccca acgttgttta tgtttcactc gttcctcctg
     9661 acactgcgtc tatcgaacac atttttcgcc agtgtggcgt tcagtaagat aaagccgctt
     9721 cgcattccat gctaatatag gccaacgcaa ttcatatagc cgttgatacc taatgtgatt
     9781 acactagtaa aatatattgt tactttacta tcgtttaggt gcgctgaatg aatctgcgcc
     9841 ctgaattctg gtaaaaaaca ttatcgtaaa ttaccatttc tttcaacagc ttactagtaa
     9901 acaagaagtt agcctccgtg aatataaacg tcgccgaatt gttaaatggg aattacattc
     9961 tgttattatt tgtggtcctc gcgcttgggc tatgtctcgg aaagttacga cttggttcga
    10021 tccaactggg taattccatt ggcgttttag tcgtatcgct gttattaggc caacaacatt
    10081 tcagcattaa caccgatgcg cttaatcttg gctttatgct gtttattttc tgcgtcgggg
    10141 tcgaagccgg accgaacttt ttttccattt tttttcgcga tgggaaaaat tacctaatgt
    10201 tagcactggt gatggttggc agtgcgctgg tgatcgcctt agggttaggt aagctgtttg
    10261 gctgggatat tggcctgacg gccggtatgt tagcaggctc tatgacgtcg acaccggttc
    10321 tggtcggtgc tggcgataca ctgcgtcatt ccggcatgga aagcaggcag ctctcactgg
    10381 cactggataa tctgagcctc gggtatgcct taacctattt aatcggtctg gtgagtttga
    10441 ttgttggtgc gcgttacttg ccgaaattgc agcatcagga cttacagacc agcgcccagc
    10501 aaatcgcccg cgaacgtggc ctggacactg atgccaaccg taaggtttat ttaccggtga
    10561 tccgcgccta tcgcgtcggc ccggagctgg tggcctggac cgacggcaaa aatctgcgtg
    10621 aactgggtat ttatcgacaa accggctgct acattgaacg tattcgacgt aacgggattc
    10681 tggcaaatcc agacggtgat gccgtgctac aaatgggcga tgaaatagcg ttggtaggct
    10741 atcccgacgc ccatgcccga ctcgatccca gcttccgtaa cggcaaagaa gttttcgatc
    10801 gtgaccttct cgatatgcgt atcgtcactg aagaagtggt cgttaaaaac cataacgctg
    10861 taggtaaacg tctcgcacaa ctgaagttga ccgatcacgg ttgcttcctt aaccgcgtca
    10921 ttcgtagcca gattgagatg ccgatagatg acaacgtcgt gcttaacaaa ggtgacgttt
    10981 tacaagtcag cggcgatgcc cgccgcgtaa aaaccatcgc cgatcgcatc ggctttatct
    11041 cgattcacag ccaggtcact gacctgctgg cattctgcgc cttctttgtt attgggctga
    11101 tgatcgggat gatcaccttc cagttcagca cattcagttt cggcatgggg aacgctgccg
    11161 ggttgttatt cgccggaatt atgctgggct ttatgcgtgc taaccacccg accttcggtt
    11221 acattccgca aggtgcatta agcatggtga aagagttcgg cttgatggtg tttatggcag
    11281 gcgttggtct gagcgccggt agcggtatta ataacggtct gggcgcgatt ggcggtcaaa
    11341 tgttgattgc cggattgatt gtcagtctgg tgcccgtggt tatctgtttc ttgttcggtg
    11401 cttatgtatt gcgaatgaac cgcgcactgt tgttcggcgc aatgatgggc gcacgcacct
    11461 gcgcgccggc aatggagatc atcagtgata cagctcgcag taacatcccg gcgctgggct
    11521 atgcgggcac ctatgcaatc gccaacgtcc tgctgacgct ggcagggaca atcatcgtca
    11581 tggtatggcc aggattagga taaaactgaa gttgccctgc aaatgaaatt tttttgcaca
    11641 accgcagaac ttttccgcag ggcatcagtc ttaattagtg ccactgcttt tctttgatgt
    11701 ccccattttg tggagcccat caaccccgcc atttcggttc aaggttggtg ggttttttgt
    11761 tatctaaatc cccaaaatct ccattctctc ccctgtccaa agccttatct gactgtcctc
    11821 tcttccagaa aatcattctt tttcgtacaa agaacaaata gttattgaga agcttattta
    11881 aaaacctaaa caaaatcggt aatattcctg gctttgcaat acacagattt tttatatgga
    11941 tattaacgag ctaaaagagt gccttcatct ggaagtcatt ggtaaaagta ggaaattcac
    12001 atggcgtaaa gtcattgtaa gggcgatgaa acatcggcgc gttcgttact tattctggtg
    12061 gcgtatcgct aaatacggcc atgagaaagg tggttactgg cggaaaattg ctggcaaaat
    12121 tgagcgaaaa atccttgata gttatgacgt taaaatccca ttagtcgttg atataggcaa
    12181 agggttggat atttcatact taacgggagt tgttatcggt cataacgtaa aaattggtga
    12241 gaattgttcc atcaaacccg gcgtaaccat cggcctacgt ggccattttg acgaaatgga
    12301 tatacagatt ggcaacaatg tcaccattgg ttgcaacgcc tccattctgg gcggcaaagt
    12361 ctatattgga gataacgtca cgataggtgc acatgcattg gttttgcatg atatcccaga
    12421 aaactccatt tttatcaata aaatagaata cgagattatc ccgaaaaaag ttatagccga
    12481 aatgtgatcg ccagtgagat gattagcgct attgagggag gtgttgcgtc ttatcgacct
    12541 ctgtagctac ccagcaaccc tctcaaccat cctcaaaatc tcctcgcgcg atagcggctt
    12601 acggtcggtg acaaaatgca gcgtcatccc ctcaataaac gcatcaagcg cgcgggcggt
    12661 tccgggttca aaccattgtt cgagcgtttg ctgactgcgc tgcatccagt tttgcattac
    12721 cgtttttaat agcggttttc ggctagccag cgcgtagagc tggtacatca gctccatgtt
    12781 atccggcgtt gccacctgtg agctgtagat catatcggtg atagcctggc atgcgccttg
    12841 agcatcacta acatcgctaa aaaatgcctg atattgccgg gacatgatct cagtaaaact
    12901 gctgaacgcc tccagcaaca actcatcaat tcctgaaaaa tagtaggtca tcgatcccaa
    12961 cggtaccccg gcaagggtag caattttgcg gtgcgtaaca gcatgtattc cgtaaagttt
    13021 caccgcctcc agcgtggcct ggataatttt ttctcgccgt tgcggatcgt tagcgcgacg
    13081 catgtcattt cctctgtctg caattgtgta caaatgtaca caaccttgct aatgttgtct
    13141 tccctcttct tattttgacg tgatctatga ccgtaaattc ttcacgtaat gcattgaaac
    13201 gccgaacctg ggcgctgttt atgttcttct ttttgccggg cctgttaatg gcgtcctggg
    13261 caacccgtac gcctgctatc cgcgatattc tctctgtctc gatcgctgaa atgggcggtg
    13321 ttctctttgg tctgtcgatc ggttcgatga gcggtattct ctgctcggcg ttgttagtga
    13381 aacgctttgg gacacgtaat gtcatcctgg tcaccatgtc ctgcgcattg gtcgggatga
    13441 tgatattaag tctggcactc tggctgacat cgcccctgct ctttgccgtt ggtctcggcg
    13501 tctttggggc aagttttggt tctgcggaag tggcgataaa cgttgaaggt gccgccgttg
    13561 agcgaaaaat gaataaaacg gttttgccga tgatgcacgg tttttatagc ctgggcacgc
    13621 tggcaggcgc tggtgtcggg atggcactga cggcctttgg cgttccggca acggtgcaca
    13681 ttttattggc ggcgctggta ggcatcgcgc ctatatatat cgccattcag gcaatccctg
    13741 acggtacggg caaaaatgct gccgatggca cccagcatgg cgaaaaaagc gtaccttttt
    13801 atcgcgatat ccagttgctg ctgattggtg ttgtggtgct ggcgatggcc tttgccgaag
    13861 gttctgccaa cgactggtta cccttattaa tggttgacgg tcacggtttt agccccactt
    13921 ccggctcgct gatttatgcc ggttttaccc tggggatgac cgttggacgc ttcactggcg
    13981 gttggttcat cgaccgttac agtcgcgttg ccgtggttcg ggccagtgcg ctaatggggg
    14041 cgttgggtat tgggctgatt atttttgtcg atagcgcctg ggtcgctggg gtgtctgttg
    14101 tactctgggg actgggtgcc tcgctgggct tcccgctgac catttctgcc gccagcgata
    14161 ccggccccga tgcaccaacc cgcgtcagtg tggtagcaac gaccggttat ctggctttcc
    14221 tcgtcgggcc gccgctgctg ggctatctcg gcgaacatta tggattacgt agtgcaatgc
    14281 tggttgtact ggcgctggtt attctcgcgg ctattgtcgc gaaagccgtc gccaaacccg
    14341 ataccaaaac gcagacggcg atggagaata gttgatgagc attaaattaa ttgcggtaga
    14401 catggatggt actttcttaa gcgatcaaaa aacctataac cgtgagcggt ttatggctca
    14461 gtatcagcaa atgaaagcac aaggcattcg ctttgtggtc gccagcggga atcaatatta
    14521 tcagttgatc tctttcttcc ctgaaattgc taatgaaatt gcctttgtgg ctgaaaacgg
    14581 cggctgggta gtgagtgaag gcaaagatgt ttttaatggc gagctgtcga aggatgcgtt
    14641 tactactgtc gtggaacatt tgctgacgcg cccggaagtg gaaattattg cctgcggaaa
    14701 aaatagcgcc tatacgctca aaaaatatga cgatgccatg aaaatggtgg cggaaatgta
    14761 ttatcaccgt ctggaatacg tcgataactt tgacaactta gaagatatct tctttaagtt
    14821 tggcctgaat ctttccgatg aactgattcc acaagtacaa aaaacattac atgaggccat
    14881 cggcgatatt atggtgccgg tccacaccgg caacggcagc atcgatctga ttatccccgg
    14941 cgtacataaa gccaatggcc ttcgccaact gcagaaatta tggggaatag acgacagcga
    15001 agtggtggtc tttggcgatg gcggtaacga tattgagatg ctgcgtcagg caggctttag
    15061 ttttgcaatg gaaaatgccg gcaacgcggt cgtcgcagcg gcaaaatacc gggcaggctc
    15121 caataaccgt gaaggcgtac tggatgtgat cgataaagtt cttaaacacg aagcgccatt
    15181 tgaccaataa aaacggcagc acgcccggca acacaatacc tgaatgaagg ctgcataatt
    15241 cgcggaaatt aacccgctgc attatttcag gcgaaatgtt tatgaaaaat tgtctactgt
    15301 tgggcgcgct tttaatgggc tttactggtg tggcgatggc gcaaagtgtc accgtcgatg
    15361 tgccgagtgg atacaaagtg gttgtggttc ctgattcagt cagcgttcca caagcggtca
    15421 gcgtcgcgac tgtgccgcaa accgtctatg tggctcctgc gccagctccg gcttaccgcc
    15481 cgcatcctta tgttcgccat cttgccagcg taggtgaagg gatggttatc gaacaccaga
    15541 ttgacgacca tcaccattaa ttcgagaatg cctgatcgca caaagcaatc aggcattttt
    15601 ttacccttcg tgagaatttc ccatctgttt atcttttaaa aagataacca tcagcgacag
    15661 ccacaaaatt ccgttggcaa ggttgaagag attaaacagt ccgttgcccc cgttcagcca
    15721 ggcatgtttg ctgatttcaa taccgacggt aaagatcagc atttgcagca ttcccatcgc
    15781 cgcagaaacc gtacctttac tcatatcgct ggcaaacagg gttaatcgca ccagtcccgc
    15841 attcgccaga ccaataccga aagcataaat acttaacccg gcggtcatcc ataaatacgc
    15901 gtgcgatgag ataaccgttg ccgcagcagc gaccaacaga ccaatcataa tcggccagcc
    15961 gcccataata atcagcgaac gtacggtgcg gcgcgaggtc agacgcgcta acagcaagtt
    16021 acccgcaatt aacgccccga aaataggcac ttgcagcaaa ccatattcat agctgctcaa
    16081 ctgctcgccg gtaatgatga taatcggcga ctgggcgatc cacgccagca acggcaggct
    16141 aacgaatccc agcgccagcg cccccgccac aaagcgaccg ttcttcagca ccagcttata
    16201 gtcacgaccg agttctttca gcgacagttt ctcgcctata cgcgtggcgg tttcaggcat
    16261 ggctcgttgc agaccgaaaa aggagatcgc tgccaatgcg gcaaacaaga caaacatccc
    16321 ttcccagggc agcacatgga tccacgccgc gcccaccagt ggaccaagta gcggagcaat
    16381 cagcgccacg ttcgccatca gcgcggtgat cttgatacaa accgcctctt cgaaggattc
    16441 ctgaattgcg gcgtatccca cagcgccaat gaaacagagg cttatgccct gcaagaagcg
    16501 caacagggtg aattgttcaa tgttttgcgc cagtaatatt gccagacagg tgacaataaa
    16561 ccacaccaca cccgccagca tcaccggccg gcgaccaata cgatccgaca gcggccccag
    16621 gagccattgt aaaaacatcc cgcccgccag atacgcggtc atcgaagtag gaacccaatc
    16681 aatgcccgcc tgatattgtt ccaccacggc caacataccg ggttgaatca tatcgttgcc
    16741 gatataggtt gaaaattcgt aaagcaccag acagagaggg aaaagtaacg cctgacgtcc
    16801 aagcctggca ccggaagcta atttattatg catgcaattt cttcgccaat aataatcgcg
    16861 caaagtttaa taaaagcgca gctaacgaga aagcgaattt tgtagctgaa accgcggtta
    16921 agcacattct tacattattg cgagtatagc tacgctttct ttaagtttta tttaacctat
    16981 gcccgttaca atcacccacc gtaaacaggc cgcttgaggg aaataagacg atgccgcttt
    17041 acccagttta acctgcactt tattctcaac gagttgcctg tattggctcc cttttaatca
    17101 ctttgcgtcg ggaagttatg ctggaaaatt tgaatctctc tctattctct cttattaacg
    17161 cgacgccaga ctcggctccg tggatgatct cgttggcgat ttttattgct aaagatttga
    17221 ttaccgtggt gccgttgctg gccgcggtac tttggttgtg ggggcttaca gcacaacggc
    17281 aactggtgat aaaaatcgct atcgcgctgg cggtcagcct gtttgtgtcc tggacgatgg
    17341 gacatctttt tccgaacgac cgaccgtttg tcgaaaatat cggctataac ttcctgcatc
    17401 atgcggcgga tgactcattc ccgagcgatc acggtacggt gattttcacc tttgcactgg
    17461 catttttatg ctggcatcgc ctgtggtccg gctcactttt aatggtgctg gccgtcgtca
    17521 ttgcctggtc gcgcgtttat cttggcgtcc actggccgct ggatatgctc ggtggattgc
    17581 tggcaggtat gattggctgc cttagtgccc agattatctg gcaagcgatg gggcataaac
    17641 tctatcaacg tctgcaatcg tggtatcgct tctgttttgc attaccgatc cgcaaaggct
    17701 gggtgcgtga ctgatctctg ctaaaaagtg tagtattgag cggctcgctt caataactat
    17761 tcagagggat tatggaaaca cgtcgcgaag agcgtatcgg gcagctgctg caagaattaa
    17821 aacgcagcga taagttacat cttaaagacg ccgccgccct gcttggggtt tcggagatga
    17881 cgattcgtcg cgatctgaac aaccacagtg cgcccgtcgt tttgctcggc ggctatattg
    17941 ttctggaacc gcgcagtgcc agccattacc tgttaagcga tcaaaaatcc cgcctggtgg
    18001 aagaaaaacg ccgggcggca aaactggctg cgacgctagt agaacccgat cagaccctct
    18061 tttttgactg tggcaccacc acgccgtgga ttattgaagc gattgataat gaaatacctt
    18121 tcaccgccgt ttgttattcg ctaaatacct ttctggcgct gaaagagaaa ccccattgcc
    18181 gcgcatttct gtgcggtggt gaatttcacg ccagcaacgc cattttcaaa cccatcgatt
    18241 ttcagcaaac gttgaataat ttttgcccgg atatcgcttt ttattccgcg gcgggcgtgc
    18301 atgtcagtaa aggcgctacc tgttttaatc ttgaagagtt gccggtaaaa cactgggcca
    18361 tgtcgatggc gcaaaagcat gtgctggttg tcgaccacag taaatttggc aaggtgcgtc
    18421 cggcgcgcat gggtgacctg aaacgctttg atattgtggt gagtgattgt tgcccggaag
    18481 atgagtatgt gaagtacgcg cagacgcagc gtattaagtt gatgtattaa tgacgtataa
    18541 ccggatgacg tttcgcgcca tccggttatc agaagattaa gagaaccagc tgccgaacca
    18601 ctgatggaat ttcatcatca cgaaatccca catccgacca aagaatccgc cctcttccac
    18661 attttccatc acgatcagcg gacgctgctc aatggattta ccgttaagct ggaaatcaat
    18721 ggtgccgaca acctgacctt ttttcagcgg tgcggtaagc tgcggttccg ttaacgtata
    18781 actcgctttc aggtttttca gctgcccacg cggtatggtc actgagcccg cttcacctgc
    18841 cccgagattc acttcgctct tatcaccaaa ccagacgcgc tgagtcacaa aggtggcatc
    18901 aggtttaatt ggcgtcacgg tttcaaagaa gcggaaaccc caggtcaata atttctcaga
    18961 ctcattaaaa cggatacggt cggttttcgc ccccagcact acggagatta aacgcatatc
    19021 gccctgggta gccgaagcaa ccagattata tcccgcgcct gccgtggttc ctgtcttcat
    19081 gccatcaaca ttcagattgc tgctccacag cagacggtta cggttaggct gacgaatttt
    19141 gttgaaggtg aactctttct ctttatgaat ggcgtactct tccggcacat cgtggatcaa
    19201 tgctttaccc agcaatgcca tatcgcgcgc ggtactgaac tgccccggcg catccagacc
    19261 gtgcaccgtc tggaaggtag tgttggtcag ccccagtttt ttggcataac cattcatcaa
    19321 accaataaat gactcctggc tcccggcaac gtaatcggcc agcgcaatac aggcgtcatt
    19381 accggactgg ataatcacac ctttgttcaa gtctgccacc gaaacctgat cgcccggttt
    19441 gaggaacatt accgatgaac cacgcagtgc cggatttccc gtcgcccagg catctttacc
    19501 gaccgtcacc atatcggtga gtttaatctt atcggcctta agcgcctgcc caaccacata
    19561 gctggtcatg attttagtca ggctcgcggg atccagtttc tcatccgcat tgccttctgc
    19621 cagcacttta ccgctggcgt aatccattaa aatccatgca cgcgcatcca cgctcggcgc
    19681 ttcaacggtt tgttcggccg cgaatgccgt tggggcaaaa aggaataaaa atgcagaacc
    19741 cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc gagtatccat
    19801 tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac gcacacacaa
    19861 acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc tgccgcttcg
    19921 ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta cttcaccatc
    19981 ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat tacgctgtgg
    20041 ggtcggaata attcaaccaa cgtaaaaaaa gtattgctga cgctcgaaga actggaacta
    20101 ccttatgagc aaattctcgc gggccgtgag tttgggataa atcacgatgc tgattttctg
    20161 gcgatgaacc ctaacgggct ggtgccgttg ttgcgtgacg acgaaagtga tctcattctt
    20221 tgggaatcaa acgccattgt ccgctatctg gcggcacagt acgggcaaaa acgcctgtgg
    20281 atcgactcac cggcacgtcg tgcggaagca gaaaaatgga tggactgggc aaaccagaca
    20341 ctcagtaatg ctcatcgcgg gatcctgatg ggattagtca gaacaccacc ggaagagcgc
    20401 gatcaggccg ccattgatgc cagttgcaaa gagtgcgacg ccctgtttgc cctgctcgat
    20461 gcggaactgg caaaagtaaa atggttctct ggcgacgagt ttggtgtggg cgatatcgct
    20521 atcgcaccgt tcatctacaa tttgtttaat gttggcctga cctggacacc gcgtccaaat
    20581 ctgcaacgct ggtatcagca actcactgaa cgccccgcag tgcgcaaagt ggtgatgatt
    20641 cccgttagct aattgcgtgg gctaacttta agtaattccc cactggactc gtcggtgaga
    20701 acgtataaat aaccgtcggg tccagtgcga acatcacgaa ttcgctgccc tctgtccgtt
    20761 aaaatacggc catcttctgt tactttgtcg ccgttgacgc tcatcacaat gacatcttta
    20821 tctttcagcg cgccaataaa taatttttgc tgccactggg ggaatttatc gctgttatag
    20881 aaggccatgc cgctcacggc gggcgaatct ttccagtaaa aaacaggttg ctcggtcccg
    20941 gcgacgatct cccctttcgc ttccggtatc ttaaagcctg aatagttgat tccccaggtt
    21001 gccagcggcc agccgtagtt tttgcctttt tgcgggatat taatttcatc accaccgcgc
    21061 gggccatgtt cattcagcca tagtgcatta ctccacggat tcatcgccat tccttgcgga
    21121 ttacgaatgc cataagacca gatctcggcg cgcgcaccgg attcctttat aaaaggatta
    21181 tcatccggga tttcgccctg gtcggtcaga cgcaccagtt tgccctgtaa tttatccaga
    21241 tcctgcgccg tcgggcgctg attgttttcg cccagagcaa taaaaagata acctttaccg
    21301 tcgaatacca gccgcccgcc aaaatggttg ccggtagaca gttttggcat ctggcgaaag
    21361 acggtgctaa agtcggtcac ttttgagaga tcatcactta agcggccata acccacagca
    21421 gttccggctt tgccatcatc gccaacttcg gaataactta accagatgcg gcgagactga
    21481 gcaaaatcag gcgctaaaac cacgtccagc aggccgccct gcccgtgcgc ccaaacgtcc
    21541 ggaactccgg aaagcggcgc agataatcct tttcctgctt gccagtgacg caactcgccg
    21601 ccgcgcagag tgattaacat accgtgatta tcgggtaaaa aggccagtgc ccagggatgg
    21661 tcgagtttgt cttgcagtac ttcgacattt accgttgcag gagccgccca gagagcggaa
    21721 gaaagacaaa taaggggcac aaggaaaaag gattgtcgat gcataacgcc tccggattga
    21781 ctctggctta aagcgtagtc agtggaggag ataacaaatt catttttaca aaaacttaaa
    21841 catgaagggg gagacgcttt ctccccctta gttttcaggc cttctcaagc atggcgtgct
    21901 tctgcaggct ctggatactc agcgttaagc tcatcagaca attttcaagc ttatcggcgt
    21961 tgacggtaat aacagtcggg caatcatggt gcccactcat caaacatact gcggctgtcg
    22021 ctaatgcttc ttcagcatga tgaagagcac tccactcttc ctgatccaga tgaagattca
    22081 accgcagcga tttatcgtgc agttcgcgat tcagtttaaa aaagttatct cgtagatgat
    22141 tgctttcgct gacggacatg tatccttttg ccttcctcag ttgcacgtat gcggcgaggt
    22201 caacgcgtgc gtcgatcaac cggttcagca gatcgattcg ctgtctgtca acgaacatac
    22261 ttcgatcctc ctctttccag ccttgttgca attaaatagt atggctattt tttgagcata
    22321 atgctgtcca ggatcaattc ttcacgccga attaacaaaa tttacatttc cttcgcctcc
    22381 gccagcgaat gagattgcga tacaaatttt atccagaact ctgtagaatc cctgccctga
    22441 cctttcctgt aactgaatat tttttaagct atgagcaaag taactcccca gccgaaaatc
    22501 ggctttgttt cccttggctg tccgaaaaac cttgtcgatt cagagcgtat tctcaccgaa
    22561 ctccgcactg aaggttatga cgtggtaccg agctatgacg atgcggacat ggtgatcgtc
    22621 aacacctgcg gctttattga cagcgcggta caagaatcac tggaagccat tggtgaagcg
    22681 ttgaatgaaa acggcaaggt aattgtgacc ggttgtctgg gggcaaaaga agatcagatc
    22741 cgcgaagtcc acccgaaagt gctgaaaatc accgggcctc atagctatga gcaggttctg
    22801 gagcacgttc atcactacgt gccaaaaccg aaacacaacc cattcctgag cctggtgcca
    22861 gaacaaggtg tcaaactgac gccgcgtcat tatgcctatc tgaaaatttc tgaaggctgt
    22921 aatcaccgct gcaccttctg cattattccg tctatgcgcg gcgacctggt gagccgtccg
    22981 attggcgaag tattaagtga agcgaaacgt ctggtagatg cgggcgttaa agagattctg
    23041 gtgatctcgc aggatacttc cgcctatggc gttgatgtta aacatcgtac tggcttccac
    23101 aacggcgagc cggtaaaaac cagcatggtc agcctgtgcg aacagttatc gaaactgggg
    23161 atctggacac gtctgcacta cgtttaccct tatccgcatg tggacgacgt catcccactg
    23221 atggcagaag gcaaaatcct gccgtatctg gacattccgt tgcagcacgc cagcccgcgc
    23281 attctcaaac tgatgaagcg tccgggttct gtagatcgcc aactggcgcg catcaaacag
    23341 tggcgcgaaa tctgcccgga actgacccta cgctcaacct ttattgtcgg cttccctggc
    23401 gagacggaag aagatttcca gatgctactc gacttcctga aagaagcgcg tctggatcgc
    23461 gttggctgct ttaaatacag cccggttgaa ggtgcagacg ccaatgccct gcctgaccag
    23521 gttccggaag aagtgaaaga agaacgctgg aaccgtttca tgcagttgca gcagcagatt
    23581 tccgccgagc gcctgcaaga gaaagtgggc cgtgaaattc tggtgattat cgacgaagtg
    23641 gacgaagaag gcgcgattgg tcgcagcatg gcagatgcac cggaaatcga cggcgcggtt
    23701 tatctcaatg gtgaaaccaa cgttaagccg ggtgatatcc tgcgtgtgaa agtcgagcac
    23761 gccgatgagt acgatttgtg gggtagccgg gtttaattaa tctaaaggca gatctaacaa
    23821 ggatattgtt cttcgatctg cctctactac ccttcccgat gtcctaccct tacgcacaaa
    23881 atcccctgac gcataaatcg agaaaaatga atcgtactcg aggcaactct cactttttca
    23941 caccaaatta aagttttgat cgcaattact tgaaataagc gtaatggtta ctatagtaaa
    24001 cattaccaca aggatggatg tatgggtgtg tataaggcaa ggcgattcag ccaaagcacg
    24061 aaaaaacttg gtattcacga taaagttctc atggctgccg ctgaagaagt gatgcaaggc
    24121 atatgggagg ctgatttagg tagcggtgtc atcaaaaaac ggttacccct ccaacaagg
//
LOCUS       DAZUCE010000059        24094 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZUCE010000059 DAZUCE010000000
VERSION     DAZUCE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.059"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..788
                     /locus_tag="ACHY03_001968"
     CDS             <1..788
                     /locus_tag="ACHY03_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HFS3905203.1"
                     /translation="FNELIFEIDNNPLQNYCCLLTEYIKKYKMNNGLSLLDLDLFRGI
                     SSNYRVVVQLLISSCLSGDSDTIYDIQEELPFSWGWIPVSIWKDVFQKCWTYLEKQIN
                     DKTLALHILQPFIAFMNHRAHIDRRLAPIANMLLTYSESLPTGCDVLPTVSREQFNEA
                     KQMLLRNPDSFGRISIFPKELWSSAITPELKSVFNKLWIEDKYHSRLEKRFNLMLVAA
                     LLTQKDNNLIHQLSALFEFHYQQAPQQLGVIYQYYFEQAGVCH"
     gene            788..7102
                     /locus_tag="ACHY03_001969"
     CDS             788..7102
                     /locus_tag="ACHY03_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS3905204.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     PDHAEAMQIIQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADQLADKLKRLTQGTVLMSLTTTQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTRP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHYALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHADWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            7326..10184
                     /locus_tag="ACHY03_001970"
     CDS             7326..10184
                     /locus_tag="ACHY03_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS3905205.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTILMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTETDTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            10187..15121
                     /locus_tag="ACHY03_001971"
     CDS             10187..15121
                     /locus_tag="ACHY03_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905206.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAKENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKRDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPGKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEIGKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSVAGRLKTEVLFRHLPRLAIVDALRKQHCFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLAMLRIDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAEKGVSGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFHNELSLFAEVHHATMFSINVYGPQNTTPSFINMSNVYAVSAIDASFE
                     HNNAGPVPGIKDEQEIEGKIKVSWNTSGHRSRLIHIGLKELSLFARLYDSEGTPAWQA
                     RLPALHAEQLVAVLEKFANQPKRLGDLQGQYFSTVMFDETYAQRDGTILRQTQFPQDS
                     SQWVLSGPHFFVGTPFYKTPRQNCTLNSDYDCLDLLTLPDDYLPRTNYIPACDAQEYA
                     KRTPRVTWTEPGEDEPRKVTDYYRLTSRTMIGASSERTLQSAIIPKKVGHIDLGFTIL
                     PKQHEMLPLLAASYNSIVFDFFVKSTGKGHFRNDIASLLPILNFGSNETEIKCRILTL
                     NSLSVLYRELWDNFYSSAFCLLKWSRDIPQLPQDFFTNLTPEWQRNCALRSDYSRRQA
                     LVEIDVLVAQALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGL
                     PRTARKADLKNGFVFNVDSPEWTGGDCTDQAIGWDDVKHLQTGTVSVTFDDYTRSDEG
                     ERRTVTWQAPFIKPDREDDYKVAWAFFAQDKESA"
     gene            15121..21462
                     /locus_tag="ACHY03_001972"
     CDS             15121..21462
                     /locus_tag="ACHY03_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS3905207.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFSNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLIDYAKTIFDQPFTDDAVIREDRYTAAEYLQNYAIAYSQYPGPEVASALDPQGY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SAQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLGEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANFIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKDRFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNMATWRWENMDIARVQGAEHTGLLSREDREATEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLINLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            21459..23573
                     /locus_tag="ACHY03_001973"
     CDS             21459..23573
                     /locus_tag="ACHY03_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS3905208.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAPEAVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
BASE COUNT         6146 a         5683 c         6410 g         5855 t
ORIGIN      
        1 attttaatga gctaattttt gaaatagata acaacccatt gcaaaattat tgctgtttat
       61 taacagagta tattaagaaa tacaaaatga ataatggctt atccttgctg gatctggact
      121 tgttcagagg tatttcgagt aattaccgcg tggttgttca attgttaata tcatcatgtc
      181 tttctggtga tagcgatacg atttatgata tacaggaaga attacccttt tcatggggat
      241 ggattcccgt ttcaatctgg aaagatgttt tccaaaaatg ttggacttat ctggagaaac
      301 agattaacga taaaacatta gcattacata tattgcaacc ctttattgct tttatgaacc
      361 atcgtgcaca tatcgatcgt cggctggctc cgattgcgaa tatgttactt acatatagtg
      421 agagcctacc aaccggttgt gatgtattgc caactgttag tcgtgagcag tttaatgaag
      481 ctaaacagat gctattaagg aaccccgaca gctttgggcg tatcagtatc ttccctaaag
      541 aactttggtc tagtgctatt actccagagt taaaatctgt ttttaataag ctttggattg
      601 aagataaata tcactcacgg cttgaaaaac gttttaattt gatgttagtc gcagcgctgt
      661 taacccaaaa agataataac ctgatacatc aactgtctgc gctttttgaa tttcactatc
      721 agcaagcccc acagcaatta ggggtaatct atcaatatta ttttgaacaa gcaggagtat
      781 gtcattgatg gacgttcagc aactgcatta tgcgccactt ctaaatcagc ttcatcagcg
      841 tgcggtgagg tcggtggtaa gtcagttggc attgcggagc aagtcgttga ctcattatct
      901 ggaatcacaa tattttcagc ctcccggtca ggaggggtca ctgcttgcag acccggtatt
      961 cgaaagtaca ttcggctgga tgcctgcgga tgaaacaatg gaggatttgc gggacaagct
     1021 gatctccagc ccgctggttg atgcattgag caaagcgcca gtgccatttc gtcatcaact
     1081 tactgcgtgg cgaacgattc tggaagacaa aaaatccttg ttagtgagca gcggtacggg
     1141 atccgggaaa acagaatgct ttatggtacc ggttctggaa gatcttattc gtcagcaaaa
     1201 atcgacccgc cagtcactaa cagggacaca agctattttc ttgtatccgt taaatgcatt
     1261 aattaacagc caacaggagc ggcttcgcga atggacgcgc ggatttaagg ggaaaatacg
     1321 cttcgcgcta tataatggcg aaacccggca taccaaatac gaaattcagg aagatcagtt
     1381 gaaagtacct gaacaagctt tgagtcggga agccatttat gaagcaccac cttccatcat
     1441 ggtgacgaat accaccatgc tggaatatat gctgattcgt cgtaaagatg ccccaatcat
     1501 cgaaaagtct caggggatgt taaagtatat cgtcctggat gaagcccata gttatattgg
     1561 ttctcaggct gctgagttag cgctgctttt acgtcgtgtt atgcaggctt tcaatgttgg
     1621 acccggcaca gataaacctg tgcagattat cgcgacctct gccactattg gtgaagactc
     1681 tcctgaaggt aataaggagc ttgctaaatt tgtcgctgat ctcgcgggcg ttacagaacg
     1741 tgatgtcaaa gtggttcgcg gttatcgaca aataccgcgg gtatcggagt cgctgattcg
     1801 tcatgaatac cccacatctt taacttctct taaatccctg agccctcagg atctttacca
     1861 acagctttgt cattaccgtg tggcccagca attgcgtcag gcgttaactc atcccggacg
     1921 gcaggcagta cgtttgagtg agttgctcaa tgttgccaga agaacctggc cagatattaa
     1981 tcaccgtgag ctactgcaac tcctcgatct aatggcaaga tcgcgagaag gtgaactggc
     2041 ttttacgcca ttacgtatgc atgggtttat cagaacactg gcgggtctat gggcatgttc
     2101 aaacaaacaa tgctcacata aagcacatga attaaatcag agtgactggc catttggtca
     2161 ggtatggttt gagcagcgcc aatattgtga ctgtggagcg ccggtatttg aagttttacg
     2221 ttgctccggg tgtggttcag cttatttgtc tgccaaagag gagatgcgcg gtgatggaac
     2281 gaactggttg gtagcgcaac cagccgctgc cgaagtggat gagtttgctc ttgatgtaga
     2341 tgtttattct gaagatgaag acaacgacga attagacaat aactctcaat ttgatcgtct
     2401 gattgcgagc gatggtgaaa aatatatcat ttcactggaa gaaacgacgg cggggaaaat
     2461 tgatagtgaa gcgcaaaagc actatgaaat aaacctgtta agaccggaat ctcgaggtga
     2521 gggacgcaat aactcctttg cttgtgtttg ctgtggtgac actcaacgga aaaataatcc
     2581 tttattccgt ccgcttcggc tcggcgcgcc ctttttttta aatgaaatta ttcctactct
     2641 gctggagttt tctccattac cgcaaacaag agaagagcag ttaggaccgt ttaatggtcg
     2701 acgcttgctc acctttacgg attcgcgtca gggtaccgct cgcatttcag cccgcttaca
     2761 gcaggatgcc gataaagcga cgttgcgagc tctttgttac caggagctgg caaatattac
     2821 agagccttca gcaaaactca gcccggcaca ctgtgcactg ttagagaaag ttattagcca
     2881 gttttctgtc ctccctttaa aaaattccat cgacgcgctg aaaaaagcga tggctgcgaa
     2941 tgaggagctt agcccgacgg acattcaggc acttaaaatt attgagccga tggtgagttc
     3001 cttaagcccg gatcatgctg aagcgatgca aataattcag gctaactctg gcaatcagca
     3061 gaagaaaacc atgtcatggc aaacgctgtc tgaacagctg gttggcagtg tggaccttgc
     3121 cgatagaatg agaggcagtt ttaaagaatt gtcggggctt gatcttacta aacaacagtt
     3181 tgcagatttt tgtttataca gcgagtttgg tcgccgccct aaaaatgcct ggaccctcga
     3241 aagtctgggt ctggtagcca ttcactatcc atttattgat aaagttacaa cctgcccgca
     3301 ggactggaag acattattac ccgatcccga taagcaatta gcggaatggc gtaatttatt
     3361 gaagattact cttgatttct ttatccgtga aaacagtgcc gttttttacg aaaacgagca
     3421 ataccctcgc tggatggggg cacgtttccc ggtaaaaatg ctccaggggc cagataaaaa
     3481 aaacaaaggg cagaaacgtg atcaactttg gccacaaata cgcgatcgcc attataaccg
     3541 ggtcattaag ctattaatcg cagtctttcc ggcaatacaa cctgagcagg ctcactggaa
     3601 atcgttagtt aaccacttaa tgattgaggt ctgggatgca attcgaccct gcttacgtca
     3661 gtttgagtcg ggttatcagc tggatattaa acaacaggct gaattttaca gtccaaagca
     3721 agtgtggcgt tgcccctata ctcgacgtgc gttagacgtc acgttgctgg gatactctcc
     3781 gtatttgccg ggtagcaaag aaattgcacc tgaaaaagca gtgttgattg aaatgcctga
     3841 gcttcctgta cgtcactggc gtttgtctgg tggtggagaa atcgcccgag aagaacgtct
     3901 ggagtggttg gaaagtaatg cgctgattca acatgcccgc gaggaaggat tatggtctac
     3961 tcgcagcgac cgactggccc tgaaagatag ctggtatcgt ctggaggaac actctgcgca
     4021 gagaacgcct gagcagaacc agtttaatga aaagcaattt aagtcaggca aagttaatgt
     4081 gctgaactgc tccacgacca tggagatggg ggtggacatt ggtggtatga gtttggtggc
     4141 aatgaacaac gttcctccag ctccggcaaa ttatttgcag cgtgcaggtc gcgcggggcg
     4201 tcgtggagag agtgcatctg ctgccattac gttatgtaaa aacacggcgc atggaatgga
     4261 ggtattcaaa gaccccctct gggcgttcaa cactactgcc agtgcgccac gggttcgttt
     4321 tgggagcagt agtattgttc aacgacatgt taatgccctg gttttaggtc tgtttttacg
     4381 agctgaagtt cctgatgcga caaaactgag ttgcaaatgg ttctttgaag gtgatgagag
     4441 ccagtgtctt cgttttctcc attggttaaa tcaccaggcc gatcagctgg cggataaact
     4501 taaacgcctt actcagggaa ccgtcctgat gtcattgacg acaacccagc tcttgacccg
     4561 cacacaagca atgatgcagc aggttgatat ccgctggaga agccaacttg cgatactgtt
     4621 ggaaaatatc gaagcgttga aggcggataa cagcgcgtgg gaagaaacac cagcaggtaa
     4681 agccatcgct tatcagcttc gtgattatcg cggtgcatat ctgttttcta aactcattag
     4741 tgaagcgttc ctgccggggc atggtttccc ggttggcgtc gtcaatttca attatctgac
     4801 ggcagatgag ctggaaaaac gtcgcgcaat aaaagccact caggctgacc ctaatgaagg
     4861 gggagaaagc ttttctcgtc gtattgaaaa attgcccagc cgtgatttgc ctacagcatt
     4921 acgcgagtat gcgcctggtg cagatgtggt attgggcgga aaagtatatc gttcctcggg
     4981 cattatgctt ggcaaggtgc ttgccagcgg gcaggagctg tcaggcgatc atcatattcc
     5041 gtggttctgg cattgccgta aatgtggtgc gggggcaacc agtactaccc gtcctgtcga
     5101 atgtagtcac tgcaaagcgg atatacagca acttgatgtg aagcgttatt tacagcctgt
     5161 tggttttgcg acagatattc gttatcaggc acataatgat gtatccatgc ctgcacaact
     5221 tccctggaaa gatccacgtg ttcttgtacc gtcttcggtg tgggtttctt tacccgatgc
     5281 tgggctagga cgttaccgtt ttagccatag tggggaattg ttccacttca gtgaagggga
     5341 atttgggcac ggctacgcaa tttgcctctc atgtggtcgg gcagaatctc aaactcaacc
     5401 ccagcgtacc cctgaaaatc taaagaaccc agagcgggaa aatacgcatt acctgttgcg
     5461 agggggcagt aacgatcgtc aggggagcaa taaattgtgc cacggtcatg tgcataaaga
     5521 tctgtggttg ggatatagca gtcgtacaga tatggttgag ctgcaactca atgatgataa
     5581 tggcctgttg atccgtgatg aggttgccgc gcgttccctt gcggtcgctt tgcgtgaagg
     5641 gttagcgcat aaattgggta tcgaaaatac tgagctgggc gtgacgacgc agcaagcgcg
     5701 cgatatcaat ggctataccg gatattccat cttcatttac gacaataatg cgggtggtgc
     5761 aggttatgca gtgcaattga tcgaccattg ggctgatgtc tttaattacg ccagaaaatt
     5821 gctcgattgc agttgcgata aattctgtca ccactgcttg ctcagctacg acagtcagca
     5881 ttatgtcaac agattagatc gtcactatgc attaaccctg cttaccaatg tacggttgca
     5941 gcgacttaac ctcgcgccag aatatcagta cttcggcgat ggtagccggg ttgaaactaa
     6001 ccctctgagt ttacgtattg cgcaatgcct gaacagcgag atttatgact cctgctcgct
     6061 ggttcttgcg gggccgcagg aacagtggga ctttgcccaa tggccattat ttaaagagtt
     6121 gctccagttt gcctcctccg gtgggaatgt tgagttgctg gtggccacgc cgctggcaaa
     6181 cttaactgat agctccagac atcagctttc cgcgttggcg gcgatgccgg gaggtcgctt
     6241 acaagtgaaa tccattgcca ctgcgcagct catgcaagga aaggggcgct ggcttgcgca
     6301 ggtgactcgc gaagggcaaa gccaacagtg ggccgcagac gacagcgcta ccgttgcacc
     6361 tggtgaatta tggggacaaa gtgcgtcttc accagtagtg acacttaaag gaacgtccgg
     6421 aaaaaccttc tcgggtcaga ccttaagtgc agaagattta ctgcccgcct tacccaccgg
     6481 ggcggtgcgc atcaatctct gtgaacaact ggacgggccg ctggaaggtt ttggttcgcg
     6541 tttctggtcg ttagtcacgc aacagcacgc tgactggaag caggcattta cccgccacaa
     6601 agagataacg catgttgagt acagcgatcg ttatcttaat agcccattca cggcgcgttt
     6661 gttaggtgaa atcttaaccg agctggttga acagggaatg gcggaacgcg ccagcctcac
     6721 tgtttgcgtc aaaaaattgg attacaactc tcgccaacac gacgctctct ataacgcgtg
     6781 gcttaatgag gaagatcgtc agcaggttgt gaccacttta cttgaagaag gatatctggg
     6841 gcctgcgtgg ccgggagcaa ttagctggtt aacgggggat aaccagagta cggagcatgg
     6901 acgagaactg acagtcacct tcagcgatgg cagtcagcat tatgtcctgc tcgacatggg
     6961 gcttagctac tggcgctgta ttgaagatac cttctttgat tttgccctgc gagtaccgca
     7021 gcaagttgaa cggctggcaa acacgcgagc gcgagctgtt gccccgggta atgaccttag
     7081 gagttatatt atcgctggat aaataattgc tcccctgggg atatcttcca ggggagttga
     7141 cccagctcac tttttaataa tttttgtttg aaaatcatgc gcaatttatg cgcattccct
     7201 cccattttgt cactttgccg ggggccttca tgatatcttt aaggccctct tttttatcgc
     7261 agtgtgccac gccacatttt tcaggatgac gttaccgcca ctgccccaaa tagacagaaa
     7321 aaccgatgaa taagcaaaat tatgcacctg gtatgcgggt ggttattcgt gatgccgaat
     7381 ggcgtattcg ccgggcggat gacagtggtg atggtgggta tttgctgacc tgcgatggta
     7441 tttcagagct tgtgcgcggt aaagaagggt tatttctgac caaactggaa caaaaagtag
     7501 agatcctgga tcccgcaaaa acgcatttgg tggaagatga atccgccaat tatcaagcgg
     7561 cacagttgta tattgaaagt cagttacgcc aacgcgtacc gacagacagt aaagtccatt
     7621 ttggtcatct ggcggcgatg gattccatgc cgtttcagct cgatcctacg cggatggcgc
     7681 tggcacaacc gcgtcagcgg atactcattg ccgacgctgt cggtctgggt aaaacgctgg
     7741 aagcgggtat tctggtttcg gagctgatcc gtcgtgggcg cggaaaacgt attctggttc
     7801 tggcggtcaa atcgatgctg acacaattcc agaaggagtt ctggagccgt tttgcgatcc
     7861 cattgacccg cctggattct gctggtttac aaaaggtgcg taaccgcatc ccaaccaacc
     7921 acaacccgtt ccactatttt gacaagacca ttatctccat cgatacgctg aaacaagaca
     7981 ttgaatatcg tcaccatctg gaaaatgcct ggtgggatat tattgtcatt gatgaggccc
     8041 ataacgttgc agaacgtgga accagttcgt tacgcagtaa actggcaaaa ctgctcgccg
     8101 ggcgtagcga tacgctgatt atgctttcag caacgccgca tgatggtaag gctgaaagtt
     8161 ttgcaagcct gatgaacatg cttgacccga cggcgatcgc caacccgaaa gagtatgaat
     8221 acgccgactt tgccgataaa aatctggttg tccgtcgctt caaaaaagac gtgaaagatc
     8281 agatgtccgg cgagtttccc gagcgcaata ttgtcaaatt gactcgcctg gcatccggtg
     8341 ccgaagaaga agcataccgt cgtctggttg agagccagtt ccgtgatgat gacgatgaac
     8401 aggcccagtc taacaaaggt cgtctgttca agataaccct cgaaaaagcg ctattttcca
     8461 gcccgatggc gtgtgccagc gtcgtggcga atcgtctgaa gcgtcttgag agccgtaaag
     8521 atcataatag ccagagccag atcaacgaac tggagtcatt gctgttggcg ctgaataaca
     8581 ttgatgccag ccagtttagc aaataccagt tgctgctcga caccatccga aaagacctcg
     8641 cctggaaagc caataacacg gaagatcgcc tggtgatctt caccgaaagt attaaaacac
     8701 tggagttcct cgaacagcaa ctgcgagcgg atctgaaatt gaaagatgac cagatcgcca
     8761 cgctgcgcgg cgatcagggc gatacgatat tgatggaaac cgtagaagcc tttggtaaaa
     8821 cgcagtcgcc gttgcgtctt ctggtttgtt cggatgtggc atctgaaggg atcaacttgc
     8881 atcaccttag ccacaaaatg attcactttg atattccgtg gtcgttgatg gtattccagc
     8941 aacgtaacgg gcgtattgac cgctatgggc aaaaacatca gcctcaaatc cgctatctgc
     9001 ttactgaggc cagcgaacca cagatcaatg gcgatatgcg cgtactggaa gtgttgatca
     9061 acaaagacga acaggcgcag aagaacattg gggacagttc ggagtttacc ggcaaattta
     9121 cccaggaaga ggaagaagag caggttgcag agttcatgat gcaggatgat ggtgccagcc
     9181 tgtttgatca actgctgaac agcaacgtct cggaaagcgc cgaacacgat ctgtttggcg
     9241 aaatatgcag tgcggtttcc agcgatgcct caatggtcac cgaaacagac accagcttgt
     9301 ttgccagcga acaggcgtac tgcgaaagag cattgggtta cctgaaagcc agtggtcaaa
     9361 ctatccagta tgaaaccttg cctgataata ccttgtcgct ggtggcaccg gaggagttac
     9421 gccgccgctt caaccagcta ccgcctgaaa ttgccccgga gaactggcag ctctatttaa
     9481 gtcaggataa aaccgtcatc accgacgcga ttgcgcgcgc tcgcggtgag caacatgcct
     9541 ggcccgatgt gcagtatctc tggcaaatca acccggtagt gcagtggctg gacgataaaa
     9601 tctcttcggc ttttggtcgt catcaggctc cggttatccg gctgccatac ctgcttgaac
     9661 ccgatgaaga tcacttcatt ctttccgggt tattcccgaa ccgtaaatca catccgatgg
     9721 tgaacccgtg gatagtggtg agctttaacc gtgaatcgct aatcggcagt cagcctttcg
     9781 ctgagttttt acaacgtcat ccgcagttga gtaacaagct gactaacagc ggcggtaaag
     9841 atcgtaatca ccaacgccag caggatttac tggaagcggc tattgctcat gcccgggagg
     9901 tattcatcca tgatcggaat gcgtttgaaa cgcacattaa ccagcaactg aatgagcatc
     9961 tgcaaaagct ggacgttttg cgtgggcggc agttgagcca acttgagctg gattttgccg
    10021 ataacaaaca gcaattgtca gtcaagcaga gccgtaaaga gcagagacaa cgcgaaatcg
    10081 aacacaattt tgacagctat atcgaatgga ttgaggacac catgacgact gaaaaagaac
    10141 cctacattca ggtaattgct gttatcaccg gagcggaggg ttaatcatgg cgctggtcgg
    10201 tattaataac gaaaacgaat tttactctaa ccattatttg ggtgaggttt tcaccagtga
    10261 tatccgcgat gtgctggaac cctggatagc caaggaaaat gcagcgcgtg aagcggagcg
    10321 tgccgctcgt gaacagggta aagacgtgga gccgggatac cgcgctccgt ggaatcagtt
    10381 taacagtctg gcgactgagt ttttccgcaa acttgccgag cacgaaaaac agcgtcagat
    10441 cccgcagcgt ctggccgatc aacgtaatcg ctggcagcca ttgttaaagg cgctgggcta
    10501 cgaaattacg ccacagatcc aaatgctgga tgacgataca ccgctgccgg tactggcgcg
    10561 ttacaacagc actgacggta gcccgtggct gtggattgtt gaagcacacg atcaggaaga
    10621 aggaacgctg gatccgctgg cgctctcttt actgaccgcg caattcccgg cggataccga
    10681 caaacataag cgcgacagcc tgcgcaaaaa agccaacggt gaatatcgca gctggcagga
    10741 tctgctctct acggcggtct tcacccaaaa tgaaccgccg cgttttgtgc tgctgctcgg
    10801 taaccgtcag ctcttgttgt tggaccgtac taagtgggcg caaaaccgtc tgctacgttt
    10861 tgattttgaa gagattttaa gtcgtcgtga aacggatacg ctgaaagcga cggccgtgtt
    10921 gctacataaa gattctctgc tgccgggcag tggggcacct tatcttgact cgctggatga
    10981 caattcgcac aaacatgcgt ttggtgtttc ggaagatctg aaatatgccc tgcgcgaaag
    11041 cattgagttg ctgggcaacg aagcgatgca ttatctgatc gaccgtggcc tggcaaacta
    11101 taccggtaac cgtgcggtgg acccggatga actgagccgc gaatgtctgc gttacatgta
    11161 ccgcctgctg ttcctgttct acattgaagc gcgcccggag ctgggttatg cgccaatgac
    11221 cgccaaaacc tatctgcaag gttacagcct ggaaacgttg cgcgatctgg agatgatccc
    11281 gctgaccagc gaagaagatc gcaacgggcg ctacttccac gacagcctga atatgctgtt
    11341 taaactggtg cgcgaaggct acaacggcgg cgtgaaaatg cagagtgacc tggagagcgg
    11401 cgaccggatc accatccata gtcatcagtt cagcgtcccg cgtctggaaa gtcatctgtt
    11461 tgatgccaac aacacgcgca ttcttaaccg cgtggtattc cgtaacgaaa ccctgcaaca
    11521 gattatccag gcgatgtcgt taagccgccc gggcaaaggg cgctttaacc gccgcggacg
    11581 tatttcttat cgccagttgg gtatcaacca gttgggggcg gtgtatgagg cgctgctctc
    11641 ctatcgcgga ttcttcgcca gcgaagatct ctacgaggtg aagaaagccg gggaagagtt
    11701 taacgagctg gagacgggtt acttcgtcag taaggatgag attggcaaat accacgaaga
    11761 cgagaaggtc tacgagaaag acggcagtct gcgcattcac cgcaaaggta gctttatcta
    11821 ccgtatggcc gggcgcgacc gtgagaaatc tgcctcttat tacaccccgg aagtgctgac
    11881 ccgctcactg gttaaatatg ccctgaaaga actgtttaag gagcagattg atcccattag
    11941 cgatccgcac gccaaagccg acgccatctt aaacctcacc gtgtgcgaac cggcgatggg
    12001 cagcgcggcg ttccttaacg aagccatcaa ccagctggcg gaagcgtatc tgttccacaa
    12061 gcagcaggcg gaaggtcgcc gtattccgca ggatcgttac acccaggagc tacagcgggt
    12121 gaaaatgtac attgccgata acaatgtttt cggcgtggac ttaaacccgg tggcggtgga
    12181 actggcggaa gtgtcgctgt ggctgaacgc catcagtggc gatgcctttg taccgtggtt
    12241 tggttaccag ctgcactgcg gtaactcgct ggtgggcgcg cgccgtcagg tatttaacaa
    12301 gagtgaactg acctacaaaa aagccaaaga tccgagctgg cttaacagcg agccggtcga
    12361 actggcgatg aacacgccgc gtgaagagac gcagattttc cacttcctgc tgcccgacgg
    12421 cggtatggct aactacagcg ataaaactgt taagcagcgt tatccggatg acttcaaagc
    12481 gctggacagc tggcgcaaag agtttattaa aagctttgcc gggcatgaga ttgctgatgt
    12541 gcagcgtatc agcgaaaagg tggaagctct gtggaacact tatcgccagc aacttaaagc
    12601 agaacgtctg aaaaccgccg acagctaccc ggtgtggccg gcagaaaaca gcgagcagac
    12661 gcgttcttcg ctgagcagta aagatgaaac ctttagcggt cgtcttgaag ataacagcgc
    12721 ctaccagaag ctgcgttggg tgatggacta ctggtgcgcg ctgtggttct ggccgatcga
    12781 caaagccgat gagttaccgg atcgcggcac ctggttgttt gagattgaaa ccctgctcga
    12841 cgggattgta atcacggaaa aagtcactga agttgcggag cacaccaccg gcgatctgtt
    12901 tgccgaagaa ggcctgctgc gagaagagtc ttcgctgttt tctgttgctg gtcgtctgaa
    12961 aaccgaggtg ttgttccgtc atttgccgcg tctggcgatt gtcgatgccc tgagaaagca
    13021 gcactgtttc ttccactggg atctggagtt ctgcgacctg tttgccgagc gcggcggttt
    13081 tgacctgatg ctcggaaacc cgccgtggct gaaagtggaa tggcaggaag cgggcgtgct
    13141 gggtgattac gagccggaat ttgtgctacg taagctgagt gcatcgaagc tggcgatgtt
    13201 gcgtattgat acctttaacc agatcccggc gctggaagcg gcctggcgca gcgagtatga
    13261 aggctgtgaa gggatgcaaa acttcctcaa tgcgcagcag aactacccgg tactgcgcgg
    13321 tgtgcagact aacctgtata aatgcttcct gccgcaggcc tggcgattag gggcagaaaa
    13381 aggcgtgtca gggttcctgc acccggaagg gatttatgat gacccgaaag gcgggcaact
    13441 gcgtgcggcg gtatatccga ggctgagggc gcattttcag tttcataatg aattgagtct
    13501 ttttgccgag gttcatcatg caactatgtt cagtatcaat gtctatggac cgcaaaatac
    13561 aacgccgtct tttattaaca tgtcaaacgt ttacgcagta agcgctattg atgcctcgtt
    13621 tgaacacaac aacgctggtc ctgttcctgg tatcaaagat gagcaggaaa ttgaaggcaa
    13681 aatcaaagtc tcctggaata cgtcgggcca ccgttcacgt ctaattcata ttggtttgaa
    13741 ggagctctct ttatttgctc gtctgtatga cagcgaagga actccagcct ggcaggcacg
    13801 tttgccagct ttgcacgcag aacaacttgt cgctgtactg gaaaagtttg ctaatcagcc
    13861 gaagagatta ggtgatttgc aggggcagta tttttcaacg gttatgttcg acgaaacata
    13921 tgcgcagagg gatgggacaa ttttacggca gactcaattc cctcaagatt catcacaatg
    13981 ggtactgtct ggccctcatt tctttgttgg gacgccgttc tacaagactc cacgccaaaa
    14041 ttgtacgttg aacagcgatt atgactgcct ggacctgcta actttgcccg acgattacct
    14101 gccgcgcact aactacattc cagcatgtga tgcacaggag tatgcaaagc gcaccccacg
    14161 cgttacttgg actgaacctg gtgaagatga acctagaaaa gtaacagatt attatcgatt
    14221 aacctcaaga accatgatcg gggcatcttc ggagagaacc cttcaaagtg cgatcattcc
    14281 taaaaaagtt gggcatattg atttaggatt cacaatactt cccaaacagc atgaaatgct
    14341 gccattattg gcagcatcat ataactcgat agtttttgat ttttttgtga aaagtaccgg
    14401 aaaaggccat ttccgtaacg atatcgcatc tttgcttccg atcttaaatt tcggtagtaa
    14461 tgaaacggaa attaaatgca gaatattaac tcttaactcg ttaagcgtat tatatcgtga
    14521 gctttgggat aatttttatt catcagcgtt ttgtttatta aagtggagcc gcgatatccc
    14581 ccagctccca caagatttct tcaccaatct gaccccagag tggcagcgta actgcgcctt
    14641 acgctctgac tacagtcgtc gtcaggcgct ggtggaaatc gacgtactgg tggcgcaggc
    14701 gctggggtta accctcgaag aactgcttac catttatcgc gttcagttcc cggtgatgcg
    14761 gcagtacgaa gcggacacct ggtacgacca aaacggccgc atcatcttta ccccaagcaa
    14821 agggctggtg ggcgtcggtc tgccgcgtac cgcccgtaaa gctgatctga aaaacggctt
    14881 tgtctttaac gtcgacagcc cggagtggac cggcggtgac tgcaccgatc aagccatcgg
    14941 ctgggatgat gtcaaacatc tgcaaaccgg taccgtcagc gtcacctttg atgactatac
    15001 ccgcagcgac gaaggcgagc gccgtaccgt cacctggcag gctccgttta tcaagccaga
    15061 tcgcgaagac gactacaaag tggcctgggc gttctttgca caagataagg agagcgcctg
    15121 atgttgccgt ccgttgtcag tcggcaggtc gccgacagcg ttgccgcctt tttacgggcg
    15181 gcgttcccgc taaacagccc gctgtttaac ggtgagaata acgataatgt ctcaatgctg
    15241 gagcaatttc tggcccagcc cgaaacgctg ctgaaaggac catacctttc cgcccagttg
    15301 ccgtttcgta aaagtgactt accgctgaat tttttctcta atctgacgct gccgttcccg
    15361 ccgcacgcgc atcaggcccg ggcatttcag cgtctgggaa gcgacgcgcc acagccgacg
    15421 ctggtcgcaa ccggtaccgg ctccggtaaa acagaatgtt ttatgttccc gctgctcaac
    15481 cactgcgcgg gcgcgtcaca agccggggtt aaagcgatta tcatctaccc gatgaacgcc
    15541 ctggcaaccg atcaggccag ccgtttcgcc aaaaccatcg ccagcgatcc acaactgcat
    15601 ggcaaggtga ccgtcggctt gtttgtcggc gacagcgaaa tcgaacccag caaaaaaatg
    15661 tcggcagaaa aggtcattac ctgcaagcac acgctgcgcg aaagcccgcc ggacatcctg
    15721 ttgaccaact acaaaatgct cgactacctg ctgatgcgcc ctggcgatca gccgctctgg
    15781 cgttacaacc agcccggttc attgcgctac ctggtcgtgg atgaattgca tacgtttgat
    15841 ggcgcgcaag ggtcggatct ggcctgtctg gtgcgacgtc tgaagcatca cataggtgta
    15901 gataaccaac gattcgcctg tgtcgggaca tcagcgaccg tcggcgacga gctggggcaa
    15961 ttgatcgatt acgcaaaaac aatctttgac cagccgttta ccgatgacgc cgttattcgc
    16021 gaagatcgct acacggcagc ggagtacttg cagaattacg ccattgcgta cagtcagtat
    16081 ccggggccgg aggtcgcttc ggccctggac ccgcagggtt acgctacccc ggtggcgtat
    16141 ctcaacgggc aaattccttt gtggttcccc gacagcgact tgcagcttcc ggacgatctg
    16201 gagagcgacg acgggcgtga aaagcgtatt gcgttggggt cactgttacg ccgccacagc
    16261 gtgatgcatg tcttactcca tgatctgcaa ggcgggatcc tctctgaacg agagtgtctg
    16321 gaaaatctcc aggtcatgct ggcagaaagc gccgaccatg cccgacgcgt gctgcaaagc
    16381 attttggcgc tgatcgcaca ggcacgtctc gaagtaccgg aaacggaaca cgatcggcaa
    16441 aaacgtctcc aggcgaagaa agcgcgtccg gtgctgccgt ttgtgcaact gcgctcccag
    16501 ttgtggcttc gtgagatgcg tcgtctggtc gccagcgtgg cgaaaacgcc gagactggtg
    16561 tttgccgatg atgtggcggt ggaagaccgc gaacactatt tgccggttat ccactgccgt
    16621 gactgccacg caacaggctg ggccagcctg cgtcatgggc aaagcgttca gctggaaaca
    16681 gacctcgacg gcatttatcg ccagttcttt gaaaaagggc gctccatcgt cctcgctttc
    16741 ccggataaca acgaaaaagc ggtcagtggc gcacaccaga aactgtgtcc tacctgtctg
    16801 aaactgaacc gacagtccag cgcccagtgt ggacattgcg gtcatacgga actgttgcag
    16861 gtactgatcc cggatatgct caaagagcgt aaggacgagc tggagtttgc taatgaatgc
    16921 ccgtactgca acagcaaaca ggggatttcg atcctggggt cacgggctgc cagtctgtcg
    16981 gcggtgatga ttcaccagct ttatggcagc cagttcaacg aacataaaaa gctgattacg
    17041 ttttctgact cggtacaaga cgccgcgcat cgtgccagct tctttactgc ccgaacctgg
    17101 ccgctgatga ttcgtgggca aatcgccagt gttcttggcg aaaaagagac attaccgctg
    17161 gatgcgttcg cggcgcgtgt ttgccaacaa acgcgtgaac gttcaccgga cgatgctcat
    17221 tttgcagcaa acttcatcgc accaaatatg cagtggctga atgattatca gacgctgaaa
    17281 aatgaaggca aactggcgga gaactccgat ctgccggagc tggtttccct gcgcctcgac
    17341 tgggaggtgt acagcgaatt taccctgcgc gcgcgtatcg gacgtacgct ggaagccacc
    17401 ggttatgccg ttgccggagt ggataaagat cgcttcaatc cggcaattac cgccctgcat
    17461 caacagcttt gtgaagagct gggtgatgag atgtctggtg tgagcgtcga gcaggtagcg
    17521 cacctggcgc tgggggttat ctggtatcaa cgccaggccg gggccgttat gcatccggcg
    17581 ttcgaaagtt atcgccgtga tggcacgacc tttatgatga cgcaaaaaga gcgtacctcg
    17641 cgctatatgc cttctatcgg tccgaaaacg cgtaaaccgg cttatgcgtc gtttgaaaaa
    17701 attaatggtt tccatcgcct gattggcgcg aagtctaacc ccagttggta tcagcattgg
    17761 atcaatcgca cgctgagtaa cggcaataat ctgtttatca gtagcgttgc ggaaacagtg
    17821 ttacgacgcc tgtttacggc gttgaaaata gccggagtag taaaagattt tgacaccaaa
    17881 gggcgtgaag cctggggact ggtgccttcg gcgctggtgg tgagccgtga cgtcgttcag
    17941 cttaattgct catgttgccg tgaggttgtc cgggctccgg ccgatcaagg ctggcagtgg
    18001 cgtaatgcgc cttgtctctc tttacgttgt gaaggtcgtt accaggaagt cgacaatatg
    18061 gcgacctggc gttgggagaa catggacatc gcgcgcgtac agggcgcaga acacacgggg
    18121 ctgcttagcc gtgaagatcg cgaagccacc gaaaaatcgt tctaccgcgg caaccaaccg
    18181 tggaatatca acctgttgtc ggcaacaccg acgctggaga tgggtattga cgttggggat
    18241 ctttccaccg ttttactctg ttctgtccca cctgctcagg ccaactactt gcagcgtatt
    18301 ggtcgcgccg gtcgtaaaga tggtaacgcg ttaaacatta ccgtggcaga agggaacccg
    18361 cacgatcagt ttttctttga agaaccgctg gagatgatgc agggccaggt gcaggctcct
    18421 ggtgtcttcc tcaatgcaac ggctattctc gaacgccagc tggcagcttt ttgtatggat
    18481 aactgggtga aaaccggtgt accagaatcc gctatctgta aaaacgtcaa acagatgctg
    18541 gatgagctgg agttcgggcg taaatcgggt tttccgtata acttactgcg atatatcgaa
    18601 cagcatcatg cggagattgc tgcacaattt acggcgatct tccccgatct tgccgcagaa
    18661 actcgccaac agctgttgtc ttatcttcag ggcgcgccag ggcaacgttc gctggtgcag
    18721 cgtatcgaag aagcgttaaa actgctggtg gaagatcgca aatctttgcg ctcacgcatc
    18781 gataaactca agcgcagtat cgataaactg gagaacgcgc cgcgcgatca aaactttgac
    18841 agcgatatgc gcgaactgac ttcagaacgg caggcgctga tggcgctggt caatcaaatc
    18901 aacaataagc aaacgctgaa tttcctcacg gatgaaggtc tgctgcctaa ctacgccttc
    18961 ccggaagccg gtattacgtt acgttctgtg ctctggcggc gtaaagaagg cggggaagcg
    19021 cgtgaatatc aaaacaccac tttcgaatat gaacgcccgg catcaacggc gctggctgag
    19081 ctggctccgc tgaataattt ctatgccggt gggcataaag ttgaaattga gcagatagac
    19141 ctgaaggtct ctgagccgga gaactggcgt atctgtagcc actgtaacta cagcgaaaat
    19201 atcgaccaga cgggggacca gcataaatat tgcccgaaat gtggcacacc ggggtgggcc
    19261 gatgccggac aaaaaaccac gctgctgaaa ctgcgccagg tttatgcccg cgccagcgca
    19321 cgtgatagcc agatcagcga cgagagcgac agccgtgaac ctgctttctt ccagcgtcag
    19381 ctattggtta gtttcgaaaa agaagatgta tcggcggcat acgcgattga ggaaggcgaa
    19441 gttccgtttg gttttgaatt tttaagcaaa gtcaccctgc gggatatcaa cttcggcaaa
    19501 atggcggatg atgccaatga gttgatgatt gccggagaag cgaagaagcg aaccgggttt
    19561 aaagtttgcc tcgggtgcgg catggtgcag cgtcctcgcg atcatgaacc acgtcatgat
    19621 ttgagctgca aatatcgcac ggaaccggaa aaggcgaagt tcgaagatta tctctatctc
    19681 tatcgtcagc tggagtcaga agcgttgcgt attctgctcc cggtaagcag ttacagtaat
    19741 gaccgtgtcg tggaggcttc tcttggggcg gcgatccagc tcggccttaa gcattacttc
    19801 aaggggaatg tcgatcacct gaaaggcgtt gtgtaccgcg agccggaaaa tgaaggcgaa
    19861 tcctggcggc agtatctggt gatttacgat accgtcccgg ggggaactgg ttctcttaaa
    19921 gagctgatgc gcactcccga caatttgctg aaattacttg aactggccta tcaggcaata
    19981 gtggaatgca gttgcaacaa tgatacgcat aaagatggtt gttatcgttg cgtttacgct
    20041 taccgtgacc gtgggcgcat gaagtatgtc tcccgcgatc aggcgcgtct cttgctggcg
    20101 aaaattttgc aggcaagaga ttcgatacgg gtaattgatt ccatcaaaaa catttccctc
    20161 gaagccatga tgggcagtga gctggaaaaa cggtttatta gctgcctgca agaaaataaa
    20221 aacctgattg tcagtcgcag ttacgcgcat cagggcgctg ggtggattat taacacccgc
    20281 agcgaaccga tgatgagctg gcatcttaaa gctcaggtgg atttaggcgc taaagaggcg
    20341 gtggggatcc cttgtcgccc ggattacgtg ctatatccgc ttatgcagtc tgatgccatt
    20401 aaaccggtgg ctattttcct tgatggcttt gcgttccaca aaaatagcgt cgcagaggat
    20461 gtgcaaaaac gccaggcgat tcgggatagc ggtaatttct gggtgtggac agtcacgtgg
    20521 gccgatctca aagagcctgg gcttaaacat gttcaggatg tcctgggact tgggcataac
    20581 ccggatatga aacagcctaa gttttataac ctgtttcatg ataccaactt tgcggccctt
    20641 gaagcgtcgt tccgggaacg caacagtttt gcactgctgc tggattatct cgccgatccg
    20701 ggcggaagaa cgcagttatg gcagagaatg gctgctgcac atgcctgggt gtggttggat
    20761 gtgaagaagt cacaggacgc gggaacgaaa caaaagtatg cctatgaaat gcaggaaaac
    20821 gcgccagcct ggcggctagc agagcttttg ccagacgaac cgtttgtctt tggtggttta
    20881 ctcgatagct gtaactcatc gcagcaattt attgagctgg cttcggtgtt gccacaacaa
    20941 gccattaaac cgacgaccag cgtcgcacaa atgcgcagct ggctgcgttt gcatatctgt
    21001 tttgacgatc gctatacgca ggatgacggt tatgaagcgg gactcaatgg cttctggcga
    21061 ctgataaacc tgttacagtt cctgccggat atgaccttta ccagccgtaa agccgtgcat
    21121 ttaccgcagg aaagcgttga cactaaagtt tcggcaacgc cagaggcagt accagagact
    21181 gatgagagtt gggctgagat tattgagttt ggcttattaa gcgctgaaga gatcgcgttg
    21241 cttcagtctc attctctctc tgcgccgact ctcggctacg aattgcaaaa tgatgaaggt
    21301 gaaattattg cggaagccga tctcgcctgg ccatcacaaa aatgggcatt gattattgat
    21361 aatcaagaat ttattccatt attcacaacc aggggttggc acgttgcatt cgggccaatt
    21421 gacgaaaata cattgcaaca tctgtccgga ggtgacaaat gacggtgcaa acgcccaagg
    21481 tggcactttc tgatggtttt ctgggcgcat tcgcgcggat cccaaaagca cagcaaaaaa
    21541 aggtgcagga attcatctcc aaattccgtc aggacccgac cagtaatgga cttaactatg
    21601 aaaagatcca cgatgcgcgc agtaaaaatg ttcattcggt gcgaatcgat caaacttatc
    21661 gcggcattgt tctgaaacct gaacagggtg ccctgtatat gctgatgtgg gtggataaac
    21721 acgacgaagc atacgactgg gcgcggcggc atgattgttc gattcacccg gttaccggcg
    21781 cgattcaggt cattgatatt agctatatca aaccggcacc ggaagcggtg gttgataaac
    21841 cgaaactctt cgcggcttac agcgcggaac agattctggc cctgggcgtg ccgcctgtct
    21901 ttattgatca ggtgatggcg cttaccgatg aagccgggct taatcaactt gaaagcataa
    21961 tgccagcaga agcctgggaa cctctgcact ggctggcaga agggctggat taccaggaag
    22021 tgctggaaga gtttaacgat catcgtgatg agcctgttga taccaacgac tttatggagg
    22081 cgattgaacg aagtaaacgt cgcttccatg tcgttgaaaa tgaacaggag ctgttgcaga
    22141 tgcttaatgc gcctttagaa aaatggcgtg tatttctgca tccgagccag cgaaaattgg
    22201 tggagtcgcc cgctaatggt ccggtacgag tactgggcgg tgcaggtaca gggaaaacgg
    22261 ttgttgcgat gcaccgtgcg cggtggttat ctcagcgtct ggcagataaa cccggtaaga
    22321 aagtactttt cacgaccttt acgcgtaacc tggcggcaga tattcgcgct aatctccagc
    22381 ggttgtgtac gcgagaagag atggcgcgta ttgacgtcgt taacattgat gcctggatga
    22441 gcgaccaact aaaacgccac ggttatgatt tccgcgtggt atacgacagc gatgaagggc
    22501 gacgcaaatg ctggaactat gccttgcagc aggcacctgc cgaactgggg ctacctgaca
    22561 atttctatgc tgaagagtgg caacgcgttg tccagccaaa tgcggtttac acccgggagg
    22621 aatacctcaa agtcagccgg gttggacgtg gtacagcgtt atcccgtatt caacgggcga
    22681 aaatctggcc agtctttgaa gaattcagag cacaaatggc gcgtgccaaa ctgcgcgaaa
    22741 tgggcgatgc catgcatgag gctattgttc tgtttaaaga aaaacaggtt caactacctt
    22801 atagcagtat tgtggtcgat gaagctcaag atatcggtgc cccggcattt actcttattc
    22861 gcagcctggt tcccgagtcg cccaacgatc tgtttattgt cggggatggg catcagcgta
    22921 tttatcgcaa taaggtcgtt cttggtcagt gtggtattaa cgtccgtggt cgtcgcagta
    22981 aaaaattgaa gattaactat cggaccacag aagagacgcg tcaatttgct gttgggctgt
    23041 taactggtgt gaaggttgat aacctcgatg gcgaagcgga taccagtaat gattatttgt
    23101 cattactgca cggggaaaag cccatgatta cgcatgcggc agatttcaaa gaagaggctg
    23161 caacgctcgt caagcaaatt caggcattgc tggcgaatca ggttcgttct caggatatct
    23221 gcattactgc gcgtactaaa catgcttgtg accgctacgc atcagcactg aatgacgccg
    23281 gaattgagac atttaattta ggcaatgata gcggtgacag cgatgcacgc cctggcgtgc
    23341 gggttgcaac gatgcaccgt attaaagggc ttgagttcca gtatgtgttc ctggtgggga
    23401 ttaacgaagg cgttgtgccg gaaataaaag cattagcatc cgatgatccc gttgaacagc
    23461 gtgatgcatt atttaatgag cgcgcgttac tgcatgtggc tgcaacccgt gccgtaaaag
    23521 gactgtttgt ttcatcatct ggtgtcccta gtccgctatt agtggctgac tgataatatg
    23581 tccaaaacag gccaggagcc gaaacgctcc tggtctatat aaccgttctg gcaactggcg
    23641 atatttatat tgcttagctc ccaacagcca gacaatatca acttagaaat tatattcgct
    23701 tatgcggttt gcatattgca cagtacaacc tgttttctct atatctaacg taacgtcagg
    23761 atgtggatga tttttggatg gggtgacaca ctggacaaat atagctggca cgaaaaggct
    23821 ggcatttcca gctgaccaat agtctctgga accatgatga tgataaaata aatatcttaa
    23881 gttttaaacg tatgatgcca ggattaccag aattataaac gaatatatta gttcttttct
    23941 atttgttcag gaggggtata aaaattgcaa ttgttaatag tatcgtcagg acgataaaaa
    24001 gataatcatg acaaaggcaa ttgaactaaa atgattgaca ttttacaggt ggtttgtcag
    24061 tgatttcttg ttattcataa tttattttcc gtaa
//
LOCUS       DAZUCE010000060        23955 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZUCE010000060 DAZUCE010000000
VERSION     DAZUCE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.060"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(47..1237)
                     /gene="bcr"
                     /locus_tag="ACHY03_001974"
     CDS             complement(47..1237)
                     /gene="bcr"
                     /locus_tag="ACHY03_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFS3905209.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(1265..1960)
                     /gene="rsuA"
                     /locus_tag="ACHY03_001975"
     CDS             complement(1265..1960)
                     /gene="rsuA"
                     /locus_tag="ACHY03_001975"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFS3905210.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2109..3869
                     /gene="radD"
                     /locus_tag="ACHY03_001976"
     CDS             2109..3869
                     /gene="radD"
                     /locus_tag="ACHY03_001976"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFS3905211.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            3994..4278
                     /gene="rplY"
                     /locus_tag="ACHY03_001977"
     CDS             3994..4278
                     /gene="rplY"
                     /locus_tag="ACHY03_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFS3905212.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4417..5424)
                     /gene="yejK"
                     /locus_tag="ACHY03_001978"
     CDS             complement(4417..5424)
                     /gene="yejK"
                     /locus_tag="ACHY03_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFS3905213.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRNSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCNYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5606..5833
                     /gene="yejL"
                     /locus_tag="ACHY03_001979"
     CDS             5606..5833
                     /gene="yejL"
                     /locus_tag="ACHY03_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFS3905214.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            5853..7613
                     /gene="yejM"
                     /locus_tag="ACHY03_001980"
     CDS             5853..7613
                     /gene="yejM"
                     /locus_tag="ACHY03_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFS3905215.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFAAYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7688..7764
                     /locus_tag="ACHY03_001981"
     tRNA            7688..7764
                     /locus_tag="ACHY03_001981"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7722..7724,aa:Pro,seq:ggg)
     gene            complement(7866..10457)
                     /gene="yejO"
                     /locus_tag="ACHY03_001982"
     CDS             complement(7866..10457)
                     /gene="yejO"
                     /locus_tag="ACHY03_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFS3905216.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAFAVDQKAGGAIKASTRVMEVFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            10777..11424
                     /gene="narP"
                     /locus_tag="ACHY03_001983"
     CDS             10777..11424
                     /gene="narP"
                     /locus_tag="ACHY03_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFS3905217.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(11635..12687)
                     /gene="ccmH"
                     /locus_tag="ACHY03_001984"
     CDS             complement(11635..12687)
                     /gene="ccmH"
                     /locus_tag="ACHY03_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFS3905218.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGILVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12684..13241)
                     /gene="dsbE"
                     /locus_tag="ACHY03_001985"
     CDS             complement(12684..13241)
                     /gene="dsbE"
                     /locus_tag="ACHY03_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFS3905219.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(13238..15181)
                     /gene="ccmF"
                     /locus_tag="ACHY03_001986"
     CDS             complement(13238..15181)
                     /gene="ccmF"
                     /locus_tag="ACHY03_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFS3905220.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILSTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAVLRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(15178..15657)
                     /gene="ccmE"
                     /locus_tag="ACHY03_001987"
     CDS             complement(15178..15657)
                     /gene="ccmE"
                     /locus_tag="ACHY03_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFS3905221.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(15654..15863)
                     /gene="ccmD"
                     /locus_tag="ACHY03_001988"
     CDS             complement(15654..15863)
                     /gene="ccmD"
                     /locus_tag="ACHY03_001988"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFS3905222.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(15860..16597)
                     /gene="ccmC"
                     /locus_tag="ACHY03_001989"
     CDS             complement(15860..16597)
                     /gene="ccmC"
                     /locus_tag="ACHY03_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFS3905223.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(16639..17301)
                     /gene="ccmB"
                     /locus_tag="ACHY03_001990"
     CDS             complement(16639..17301)
                     /gene="ccmB"
                     /locus_tag="ACHY03_001990"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFS3905224.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(17298..17921)
                     /gene="ccmA"
                     /locus_tag="ACHY03_001991"
     CDS             complement(17298..17921)
                     /gene="ccmA"
                     /locus_tag="ACHY03_001991"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFS3905225.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(17934..18536)
                     /gene="napC"
                     /locus_tag="ACHY03_001992"
     CDS             complement(17934..18536)
                     /gene="napC"
                     /locus_tag="ACHY03_001992"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFS3905226.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLTGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(18546..18995)
                     /gene="napB"
                     /locus_tag="ACHY03_001993"
     CDS             complement(18546..18995)
                     /gene="napB"
                     /locus_tag="ACHY03_001993"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFS3905227.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(18992..19855)
                     /gene="napH"
                     /locus_tag="ACHY03_001994"
     CDS             complement(18992..19855)
                     /gene="napH"
                     /locus_tag="ACHY03_001994"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFS3905228.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(19842..20537)
                     /gene="napG"
                     /locus_tag="ACHY03_001995"
     CDS             complement(19842..20537)
                     /gene="napG"
                     /locus_tag="ACHY03_001995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFS3905229.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(20544..23030)
                     /gene="napA"
                     /locus_tag="ACHY03_001996"
     CDS             complement(20544..23030)
                     /gene="napA"
                     /locus_tag="ACHY03_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFS3905230.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(23027..23290)
                     /gene="napD"
                     /locus_tag="ACHY03_001997"
     CDS             complement(23027..23290)
                     /gene="napD"
                     /locus_tag="ACHY03_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFS3905231.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(23280..23774)
                     /gene="napF"
                     /locus_tag="ACHY03_001998"
     CDS             complement(23280..23774)
                     /gene="napF"
                     /locus_tag="ACHY03_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFS3905232.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            23883..>23955
                     /locus_tag="ACHY03_001999"
     CDS             23883..>23955
                     /locus_tag="ACHY03_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905233.1"
                     /translation="MHTPIEVKPVAGSKEWREAWQKRA"
BASE COUNT         5868 a         6799 c         5919 g         5369 t
ORIGIN      
        1 atgcatcaac aaaggaagcc ttttagcttc ctcgttgtgc aatagatcac cgttttttcg
       61 gccgactggc gtacagacag aagagaatgg agctggttgc gcagaatgca attgaccaaa
      121 tcatcggcca tgcagagtta aaggtcgcaa gagaaagcaa tgcgccaaca attgccccta
      181 tgccaaaacg gaaggttcct gccagcgaag atgccgttcc cgccatatgg ggaaactcat
      241 caagaatgac cgccatcgca ttggatgaca ccatcgacac gcagcccaca aacgccgcaa
      301 cgccaaccac cagcgaccag aatcccagcc ccagtagcgc actgatgacc atccacgctg
      361 ccataataaa ctgtatccac aaccccgagc ggaacatatt taacgcgcca atgcggcgaa
      421 cgaagcggct gttaaagatg gtcatcacga acaggaaaac aatgtttagt gcaaagtaat
      481 aaccaaagtt ttccggcgcg acgtggttaa tttcaatgta gacaaacggt ccggcgctta
      541 agaacgagaa catcccggca aagctgaaac cactggccag catataactc aggacacgct
      601 tatgacggaa cagcgccgca aagttaccaa tagtggtacg aatgtgaaaa ggctggcgac
      661 gctccggtgg taaggtttct ttgatcagaa agaagatcat tgccgaagcc agaatcgccg
      721 ccacggcgag gatccagaag atgtaatgcc agctcagcca caccagcacc cagccgccaa
      781 ctatcggtgc catcagcggt gcaatggttg tcaccagcat gacaaacgac atcatccgcg
      841 agaactcttc tttcgggtag atatcacgca tcaaggcgtt gataaccacg ctggccgcag
      901 ccgccgccag cccgtggaag aaacgcatca caatcagctg atcgatggtt tgtgccaacg
      961 cacacgccac cgcggcggcg gcaaacacca gcgtaccgcc aagaaccacc ggcttacgcc
     1021 cgaagctgtc tgccatcggc ccgtagatta actgccccaa cgcaaagccc agaatataag
     1081 tactgagggt catctgcgta ctgcccgccg gtacgccaaa ctgcgctgaa attaccggta
     1141 gcgcgggcag atacatatca atcgacagcg gcatcaacat ggccagcagg ccaaggataa
     1201 aaacaatagc aaacgacgaa tgctgtcggg tggtcacaac gggctcctga aagtcattga
     1261 aaagttagac gacgctggca atttcttctt cagttaacgg acgatattca ccgggggcta
     1321 aatcagcatc cagcgtaatg ccgccaatac gttcacgatg cagctcaacc acatggttac
     1381 ccacagcggc gaacatgcgt ttcacctgat gataacgccc ttcgctgatg gtcagacgaa
     1441 cctgcgttgg ggtaatcact tccagcaccg ccggcttagt gagatctttt tcgttatgca
     1501 gctgcacgcc tttagcaaat tgctctgccg tatcgtcagc tacgggtgat tccagcgtca
     1561 ccagataggt cttctcgcaa tgatggcgcg gagaagtaat gcggtgcgac cactgaccat
     1621 catcagtcat tagcaccaga ccggtggtat cgatatccag ccgccctgct gcgtgcagtt
     1681 tccacgccac tggttcatca agaaaataga gcaccgttgg gtgatcaggg tcgtccgtgg
     1741 agcaaacata gccctgaggc ttattgagca tgaagtaacg tggaccgtgt tgctgcgcca
     1801 gcgggttgcc atcgtaagcg acatcatgtt caggaagcag tttgaacgct gcattacgga
     1861 cgatttcgcc atcgacggtg acacgattgc cgcggatttc acgcccggca atagcacggc
     1921 taacgccgag ttgctgtgcg ataaatttat caagtcgcat gtgtgtgatt ttgcctgtaa
     1981 aaacggaggt cgggcattga cccgaaaatc tgaactgttg tctgcccagt atagcggtct
     2041 aataacttcc ctcaagggaa aaagaaccgt ggcatactat tagcagaata atctacctac
     2101 gcgagaccat gatttttaca ctccgcccat atcagcaaga agccgtggat gccacgctca
     2161 accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggt gcaggtaaaa
     2221 gcctggtgat agcggaactt gcgcggctgg cgcgtggtcg cgtgctggtg ctggcacacg
     2281 ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg gaagccgata
     2341 tttttgccgc cgggctaaag cgcaaagaga gccacggtaa agtggtattt ggcagcgtgc
     2401 agtcggtcgc ccgtaatctt gatgcctttc agggtgaatt ttcgctgttg attgtcgatg
     2461 aatgtcaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc actcacctga
     2521 caaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt cgactgggca
     2581 aaggctggat ctaccagttc cattatcacg gcatggtacg cggcgatgag aaagcccttt
     2641 tccgtgactg catttatgaa ctgccgctgc gttatatgat taaacacggc tatctgacgc
     2701 cgccagaacg actggatatg ccagtagtgc aatacgattt cagccgcttg caggcacaga
     2761 gtaacgggct attcagcgaa gccgatctca accgcgagct aaaaaaacaa caacgtatta
     2821 ccccgcacat catcagccag attatggagt ttgctgcaac gcgcaaaggg gtgatgattt
     2881 tcgccgccac cgttgaacac gcaaaagaga ttgtgggatt actacccgcc gaagatgctg
     2941 cactgattac tggcgacacc cccggcgctg agcgcgatgt gttaattgaa gattttaaag
     3001 cccagcgttt tcgctatctg gtcaacgtcg cggtactgac caccggattt gacgccccgc
     3061 acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac caacaaattg
     3121 tcgggcgcgg tctgcgtctc gctcctggca agactgattg cttaattctt gattatgcgg
     3181 gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa agtgacaacg
     3241 ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg gggaaaacga
     3301 ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg tttgaagatg
     3361 acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc ccgcaatgta
     3421 acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta ctggttgatc
     3481 cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta cgctgtagcg
     3541 gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc acctattacg
     3601 atgaagacgg cgcggatgtg agtgagcgtt tccgtctgca aacgcccgcc cagcgaactg
     3661 ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatccct ctgcgctgga
     3721 tcaccgccgc cgatatcctc gcccagcaag ccttactgcg acatccggat tttgtcgtcg
     3781 cccgcatgaa aggtcagtac tggcaagtgc gtgaaaaagt gttcgattac gaaggtcgtt
     3841 ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat aaggcgattg
     3901 agtatagaat ctcgcccgct tttgcatacg caaagcagat cacttacctg ttgctgggtc
     3961 gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc agaagtacgt
     4021 aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaaatt cccggcaatc
     4081 atctacggtg gcaaagaagc gccgctggct atcgagctgg atcacgacaa agtcatgaac
     4141 atgcaagcta aagctgaatt ctacagcgaa gttctgacca tcgttgttga cggtaaagaa
     4201 atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct gcagcacatc
     4261 gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg gcggggtttt
     4321 ttgtatctgc agattatgcc tgattacgat attgctattt tttgcaggcc agataaggcg
     4381 ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag acgtccggcg
     4441 ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcaaggtat cggttgctgg
     4501 atcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca agccaccgcc
     4561 gctaccggca aacttcgtca actgacgcag tgtgctacgg tctgccggga agctctcttc
     4621 cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctga cgcctgccag
     4681 ctctttcgac agcgactcca gttcgatctc ttcgcctgcc tgcagctgct cattacagta
     4741 gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg cttcggcagt
     4801 gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgcgttta aaccttcgct
     4861 ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactctcc ctttcaggaa
     4921 agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga tacgcgcaac
     4981 aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat tttcgttaac
     5041 gcgcatactg ctcaggttgc tcagaactgc caccagcaaa tactccaccg ccagatagcg
     5101 atagttgcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta attcgtcacg
     5161 caggcgaccg gttgccgcgc ggctaaatgc cagaaagtcc tcttcaccct gacgctgtaa
     5221 gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt tatttttggc
     5281 gctatagacc cgatgcagtt ctgctaccat ctcaacgacg gtttctgtcg gttccagcaa
     5341 tgaattgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct ggtgcagagc
     5401 aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg gtattcaacc
     5461 accgccggtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc ggcgtaacga
     5521 gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat atgttgccct
     5581 ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagtg atgaacaggt
     5641 tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc cgaccgacct
     5701 ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca ttgccccggc
     5761 ccaacgccag gcaattgcca actcttttgc ccgcgcctta cagtcctcta tcaacgaaga
     5821 caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag cgctaccgtg
     5881 aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat attctgcttt
     5941 cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgaccacg cttgctggtc
     6001 gcatttattc ctacgtaagc attatcggcc atttcagctt cctggtgttc gccgcctact
     6061 tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg aggtttttgt
     6121 ccgtcattct ggcaacggcg ggaatgacgc tattactgat cgatagcgaa gtctttactc
     6181 gtttccatct ccatcttaat cccatcgtct ggcaactggt tatcaaccca gacgaaaatg
     6241 agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta ttgcttgaac
     6301 tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt cgacgcttcg
     6361 cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg gtgtatatct
     6421 gggccgatgc aaacttctat cgcccgatca ccatgcagcg cgctaacctg ccgctttcgt
     6481 acccgatgac ggcgcgacgt tttcttgaga agcatggcct gcttgatgcg caggagtatc
     6541 aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgttcagtat ccgttaagcg
     6601 aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact gtcgatggcc
     6661 tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct gagcaaaata
     6721 tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc tttggcctgt
     6781 tctatggcat ctcgccgagc tatatggacg gcattctgtc gacccgtacg cctgcggcat
     6841 taattactgc gcttaatcag caaggctatc agctggggtt attctcatca gatggcttta
     6901 ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc gtacgcaccc
     6961 aatccgacga gcagaccgcc acgcagtgga tcaactggct gggacgctac gcacaagaag
     7021 ataaccgctg gttctcgtgg gtttctttca atggtactaa cattgacgac agcaatcagc
     7081 aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag atcaaccgcg
     7141 tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggtagtgatt atcactgccg
     7201 gtcggggtat tccactgagc gaagaggaag aaacctttga ctggtcccac ggtcatctgc
     7261 aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat gctctgactg
     7321 atcataccga tctgatgacg acgctgatgc aacgcctgct acatgtcagc acacctgcca
     7381 gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac tgggttactg
     7441 cagcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg ctgaacaata
     7501 acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa aaaccacagt
     7561 taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac tgattaatta
     7621 taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag tattaacagc
     7681 cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg ggggtcggag
     7741 gttcaaatcc tctcgtgccg accaaaaatc ccaaaaaaaa ccaaccgtaa aggttggttt
     7801 ttttatatct gcatttaatt cgataaacag accgcgacac atcacggcct gtttattgtc
     7861 tgttatcaga acgtccagac cacacccgcc tgggtattcc acggcgattc tacccctgcg
     7921 ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga tgaccttatc
     7981 ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc atcgtcatta
     8041 actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag ccagggttcc
     8101 agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc ttcagcgcgt
     8161 aatatccggg tatttcccac gtccgcgcgc atgccgtttg ataacgtgta gtcctgacca
     8221 tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc gtcaacccaa
     8281 cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc aaacgctgtt
     8341 gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt caccacccca
     8401 tcaacatagg caccgttctg atgctcccaa ccggcataag cacccagggt atagctatcg
     8461 acattgcctt tgccgccgcg atcaaaacca atatcagaat gagagtaacc aaagaacaag
     8521 ccgcgaattg tgctgctttc ttcacgggag aaacggctat cgataccgag cgtcaggccc
     8581 gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg ggtgttaatt
     8641 gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag acgctcacgc
     8701 acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ccgccatatt cagcacatca
     8761 gtggttgaag gggtaatttg cgcgcgattc tctgccagac tccagctatg gttaccatta
     8821 tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt gcccaacgta
     8881 aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc tgccgggctg
     8941 gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt gacgttgagc
     9001 tgatcgctct gatgattagc catatcggta cgcatataaa aattgccgtt tcccgacagg
     9061 gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcactgatga acgagtcact
     9121 ggctcatcca tcataatcac atttgcgtca gacatcgcaa ggttggctaa gttgaggagg
     9181 gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atacgctatt ttcgagcgaa
     9241 acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc ctcagaaacc
     9301 actaccttac ccaccaacat tgcgggtgca taatttattt gcggcttcat gacctcaaga
     9361 atgccatcat tccctctgac tgaaacgtta accattgtgc cagcccagac gttagcgcgg
     9421 ccattgttga taaccatatt ttcagagatg gcttttgagg aatacgtgat acttccattc
     9481 tgatctgatc gaccgagatc atataccgca tttgcctgca ctctcgtccc agtatccttt
     9541 cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg cgtgtcctcc
     9601 ataacaataa gcccggaacc atcatccagt tcataatttt ttgacacacc atcttttata
     9661 ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga gacaattaat
     9721 tttccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac ctccagtaag
     9781 ccgccactat ctacagtagt attataagcg aagtcatttt cttcaactcg caaacttccg
     9841 ccgttttcca acagcatatt gttagcaata ccattcttga tttcgaactg accgagacgg
     9901 tttgttccga atacctccat gacccgcgtg cttgctttaa tagcaccgcc tgctttctga
     9961 tctaccgcaa atgctaatcc cccctcttta acctccagta gtcctccctt ttcgatagcg
    10021 gcaaggacgg taatcccatc tttttcaacg attaagcgcc cgcctttaat cgtgggatca
    10081 ttcgctgcgc cataaacctg tagcacacca ttattgaggg tagtctggtt tgccgcgcca
    10141 cccatttgaa caatttgata accatcattt aatacggagt attctgcggt gccattcatt
    10201 tcaatgtact gatacccgcc gttaatttca gtattactac ttataccaaa ttctttaata
    10261 tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac cagctggata
    10321 tctttttcca ggacaacacc atcaactgtc tcaccatact caactgttga tgccaatgct
    10381 atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca aagagaaaca
    10441 gaaccagatt gatgcattga gctttcatcc tatgaaatta attgctgtta aaagcattgg
    10501 gtacggaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg tttaaagaaa
    10561 tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct gatgcaaaat
    10621 tccgtcttta taatgaaaat gatgccaaag cgaacgacaa ggttgtagtt ttcactacat
    10681 gtccatacat aaaatggggt aacattcacg cgcctggtag cgataccaac gctacgctca
    10741 aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac gccttttcag
    10801 gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt actggagctt
    10861 gatcctggct ttgaagtggt cgccgaagcg ggcgacggcg cgagtgctat cgatctggcg
    10921 aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat gagtggcctg
    10981 gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat cctgaccgta
    11041 tcagatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg ttatctgttg
    11101 aaagacagcg acccggaagt gttgctggaa gcgattcgtg ccggagcgaa aggcagcaaa
    11161 gtctttagcg aacgcgtcaa tcagtactta catgaacgtg aaatgtttgg cgcggaagaa
    11221 gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct ggcacagggg
    11281 ctgtcaaata aacagattgc ctcggtgttg aatatttccg agcagacagt aaaagtacat
    11341 attcgcaatc tgctgcgcaa actcaatgtc cgctcacgcg tggcagccac cattctgttc
    11401 ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg gctgagattt
    11461 atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgcgg atcgatcaat
    11521 ttattgaatt cacacgattg tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa
    11581 ttaagcgcac tttgtcatca gtctggcctg atggaatcac catcgggcct ctttttattt
    11641 actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa tcaccgcacg
    11701 gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa cggcttcgcc
    11761 aaatcgctgc tgctcaaagg cattaaacgc atacatgctt aacacacgaa tattgctatg
    11821 gtccgttctc accaactggc gcagcagttc gccgccgagg cggttgtcgt tgggatcaga
    11881 tgaacgtgtc aacgcttcag cataacccag tgcggcatcg ctatttttcg gatcgaggcg
    11941 atacgcagta gcgtaagcat cggtggcgat actggcgtta cccagcgcca tgccaacgcg
    12001 gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag tacgcatccc
    12061 cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcggat ccagcgccct
    12121 gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct gctgataatt
    12181 gccagtctgg tagtagctga cgccagctac aattaacgcc actaaaatac ccggcagata
    12241 aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg cttccggtac
    12301 cacgcgtacc cgacgccgcg agcgggcgta aatgacccaa ccgccaatac caatagccac
    12361 taccggaagc acccacagca gcacggtcag cggcgttaac ggcggatcgt aagtgacgaa
    12421 gttgccgtaa cgcgccacca tataatcgac aatctctttc ttacttttgc cttcctgcat
    12481 cagttcatac actttctggc gcaggtcggt ggcaatcatc gagttggaat cggcaatgct
    12541 gttattctga catttcgggc agcgcagttc ttcagtgagc tgacggaact gttgttcctg
    12601 tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg agatcatcag
    12661 catcagcacg cccaataaaa acctcattgt gcggcctcct tgctgtattt ctcccacagc
    12721 ggcttgatct cttcttccca gacgcgagga ttcagatcgc ccgcatggcg atagcgaatg
    12781 atgccgttgc cgtcaataag gaacgtttct ggcgcgccat agacaccgag atccagccct
    12841 aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc tttcagccag
    12901 ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcggat gccctgcgca
    12961 gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaggt cgcccagacg
    13021 ttaagcagca caggtttgcc ctgagtcagc acatccgcct gataaaactg ccccggattg
    13081 tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga ttccagattg
    13141 gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc ggcaatcgcc
    13201 aggaagataa tcaacggaat taacaatact ttgcgcttca tacagcctcc ggcgcagttt
    13261 tttgcgggct cacgcgctta cgatagcgag gatcaaacag acacagcagt ccgcccaacg
    13321 ccatcatcag cccgcccgcc caaatccagc gaacaaaagg tttgtagtag agacgaacag
    13381 cccaggcacc gttttccagt tcttcaccga gtgccgcgta caggtcacgg gtgatgccgc
    13441 cgtcaatcgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt ttttccgcat
    13501 acagcaccgt ttccggcttg ccatctcgcg ttacgccgat agtcgccaca ccgccacgcc
    13561 agttcgggcc agtcacctct ttgacatcac ggaaggtaaa gcgatattca tgaatatcga
    13621 cgctatcgcc ggacttcatg cgcacatcac gctcaacgct gtagttctga ctaaaggcaa
    13681 tgccaacaat tgtcactgcc agcccaaggt gagccgccac catcccccaa taactgaagg
    13741 tggttttcgt gccgcgtgaa atacgcagca cagcttccgc aattgccagc accgcaatcc
    13801 agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg aacagccacg
    13861 gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa ttgcggattt
    13921 tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt agcgcaaacg
    13981 gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa atactgccca
    14041 gtcccaattg cttgtgcacc agcggcagca gcgtccccag caacaccacc agcatcgcgg
    14101 cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc gcattgttta
    14161 cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg ccaatcacca
    14221 gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac gcgtgtaccg
    14281 ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag aaggcactga
    14341 ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc gccagtgagt
    14401 gcatcagcgc agtccccacc agccacggca taaacgaggc gttttctacc ggatcccaga
    14461 accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc acaatgccga
    14521 gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca taagtgctgt
    14581 ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag aaacccacgt
    14641 accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac agcgggttaa
    14701 gatcgcgccc ttcaatcggg aagttcggca acgtgcgtga gaacgggtta gaggtagaga
    14761 gaatgaacag caaaaagccg acactgacca tccccattat cgccagtacg cgggccacaa
    14821 tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg ctcatcagca
    14881 gcacccacag cagcagtgag ccttcatgtg cgccccaggt agccgccacg cgataccaca
    14941 ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg ttgaccacga
    15001 aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc caggcaaaca
    15061 agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc ggatacacgg
    15121 acagcagcag cgcaatccct aacgccaggc acagcagccc gttaccaatt tccggcatca
    15181 tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca tcgctttctc
    15241 aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg cgaggatatg
    15301 attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga acagatccgg
    15361 caaaatgcct tcgtaagaga catccactga gccttcagcg tcgtaaatgg tgaaggtcac
    15421 tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc caacgcgcag
    15481 acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa tttcccccgg
    15541 cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga tagtcagcgc
    15601 cagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa tattcatgca
    15661 gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg cgccacacca
    15721 cgcagaattg cgcgatgctg catcaccgag tgcacgacca aaaccaccag cggaataacg
    15781 gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa aaattcattc
    15841 caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca ctcacccacg
    15901 gacggcgttt ttccatcagt aaaattaaat tacgcatccg catcagcgtc agcgtggcag
    15961 acagcagcaa gaagccaaaa atcgaccagc gcagcggcga acgcatcgca ggatcgatac
    16021 tttgctgcat tcgcgttgat ccctgatgca gggtgttcca ccactcaacg gagtaatgaa
    16081 taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg cccgccagac
    16141 ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac agcagcacca
    16201 gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt tttccccatg
    16261 cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc atcgccgcca
    16321 ccgccaggtt ggccattttc atctgccaga caaggccaat aaaagccgcc accgccattg
    16381 atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata atgcggtagc
    16441 tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag ccgacggtaa
    16501 gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt tgatacagcc
    16561 gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca gactcgaaca
    16621 aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg tcgcaaaagg
    16681 acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc cgtcaacggg
    16741 caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg ggatagtcag
    16801 cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg tcagcgccac
    16861 gcccggcgca ccaagaaagc caagcgtagg ggttcccagc agtagcgtta acgccatcac
    16921 ttgccagcca taaacatcca ttcccagtag cattgctacc aggggcgaaa ggatgagtaa
    16981 cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg cgggcaaggg
    17041 taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac ggaacagtcg
    17101 ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg gtgcaatacg
    17161 cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta caatcaggaa
    17221 gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca gctcaagacg
    17281 gaaaatgcgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg gcgaatttta
    17341 ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc ctgctccgta
    17401 tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc ggtaaaaggc
    17461 tcgtcgagga tccataacgt ggcacgggtc agccacagac gcgctaaagc gacgcggcgt
    17521 tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag cccggcctgc
    17581 gccagggctt ccagacattg tgcggtatcg ccatcgcgat gataaaagtg cagattttct
    17641 aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag gttttgatgg
    17701 tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc tgcgtcaggg
    17761 cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt gctaccggtg
    17821 atttgtaccc actctcctgc gttcagcgta aatgataaac cactaaataa ggttcgttca
    17881 tcccgctcac aaagtaactc tctggcttca agcataccca agcaataacc ctgttaaaaa
    17941 cctggctcga cttcacgcat atcggggagc ttgtgcgcta tccctttatg gcaatcaata
    18001 caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga tttctgggcg
    18061 gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt attgtccttc
    18121 atgcgccgcc actcattctg tgccatcgtc aggcgatgag cttcaaattt ctgcggcgtg
    18181 tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt gcgtatcatc
    18241 ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac gccgctacgg
    18301 ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttacgcat ttcgtggcag
    18361 ctaatgcaga actcttcggt attggctttt tccatcccag tgttaaagcc gccccagaag
    18421 acgatgccgc cgacaaaacc ggtcaacagc agcgtcccca gcgccagacg gctgggggta
    18481 cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt tcccataata
    18541 acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat tggcgcggta
    18601 tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac ttccgcgccc
    18661 actttgccgt cgctgtccat aaagtgagta ggactgatac gcggtgcgcc agtggtgcga
    18721 tagctttcga caccgtggca ctgcaagcag cggttggtat tggtcgttac ctgataacct
    18781 tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat ccgatcctgc
    18841 tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga ctggctaaaa
    18901 tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat cgccgtccat
    18961 tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa ctccatcgtg
    19021 tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac gtcatgcaat
    19081 cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt agcacatgcg
    19141 gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga tcgctggccg
    19201 caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc ggacaaatgt
    19261 gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg agaataagca
    19321 gcgcgccgct gccgaagccc attaccaggc tacgccccat cagggaaacg gggttgatcc
    19381 attcccagat gagcgtgcca gttaaggctg atcccaccag aatgaccacc aacagcacgt
    19441 agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg cgtaaccagt
    19501 tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat cgctttcccg
    19561 ccagggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg gcaggcagat
    19621 gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg gtgtcgaata
    19681 acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccacgga ccgctcaaaa
    19741 acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg tgactgcgcc
    19801 accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt gccattgttc
    19861 ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag tgacagcggt
    19921 aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc gcaaccggta
    19981 caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg cgtgttgcgc
    20041 tccagctcca gggtgatggc ctcatcaatt ttcgggcatt cgcgataaca aacatcgcag
    20101 cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag ccccatccgc
    20161 gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc tcgggcacac tttggcgcac
    20221 ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg cgtgcccgcc
    20281 gacaggccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc ctgaacacac
    20341 tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc gggcgggcgc
    20401 aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac acccacggca
    20461 gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc attttgaggt
    20521 ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca cttcttgaag
    20581 tccgtctctt tcgagagcgg atcggtcgcg tccagcgtta gtttgttaac cagctgtgcg
    20641 gcgtcgaaga acggcatgta caccaggccc tgcggcggac ggttacgacc gcgcgtttcg
    20701 acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc acggcgcaga
    20761 tcgcgcgctt tcgcatccag cgggtgaata aacaggaccg cttccgggaa ggcgcggtgc
    20821 agttccggca cacggcgagt catactgccg gtgtgccagt gctccaggac gcgtccggta
    20881 gagagccaca ggtcgtactc ttcatccggt gcttctgccg ccggttcgaa cggcagcgcg
    20941 aagatcaccg ctttaccatc cggtttaccg tagaacttat agccttcgcc cgctttcacg
    21001 tacgggtcgt taccttcgct gtaacgccac tgcgtttctt taccgttcac caccggccag
    21061 cgcagaccgc gcgctttgtg gtagtcatcg aacggcgcga gatcgtgacc gtgaccgcga
    21121 ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag ctcgcgggat
    21181 tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac ttccggtgtg
    21241 gcatacagca cttcgtacag cgttttgcca cgcagttccg gtttcttcgc cagcagctct
    21301 tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg ccagagatcc
    21361 gatttcgctt cgcccggtgc ctgtacctgc tgacgccaga actgagtacg gcgttcggcg
    21421 ttaccgtaag caccctcttt ttctacccac attgcggtcg gcaggatcaa gtcggcggcc
    21481 agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg atcgcgccag
    21541 cccggcatac gctcttcatt aatgttcggc ccggcctgca tgttgttggt acacatggtc
    21601 cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc gtgcagaccg
    21661 atttttgccg gaatggtgcc gctcgggata ttccacttct tctcgcagat atcacgatgt
    21721 ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc cacttcacgc
    21781 gcagtaccac acgcggaagg ctgcccggtc agggagaacg gaccgcaacc cggctgggaa
    21841 attttgccgg tcagcaggtg caggttgtag accaggttgt tggcccacac gccacgagta
    21901 tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc ggcatacagc
    21961 tgcgccagtt gttccagctg atcttttggt acgccagtca tttcagcggt tttttccagc
    22021 gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga ggcgtcagaa
    22081 cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta gccgatgtcc
    22141 gtcgcccctt tgcgcaggtt aacgtgtttg ctaaagaagt cctgatttat cgcattgttt
    22201 tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg cgtaaagatg
    22261 atgccgttat ccgccagctc gaagctacga tgctggtagg ttgaaagcac cgccacggtg
    22321 acgtcctggt tagagagacg acggttagtg atgcgtgacc agaggatcgg gtgcatctcc
    22381 gccatgtttg cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc atagcagccc
    22441 atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc catacagtga
    22501 cgcgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt taaacagctt ggacgcggca
    22561 taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc cggccctttt
    22621 tctttcaggg cggttttgaa tttctcttcc atcacatcga aggcctgatc ccaggtgatt
    22681 ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag cggctgcgtc
    22741 aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca gttcaggcca
    22801 cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg ctgcgttccg
    22861 accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca tttaatggct
    22921 tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc agccgccgca
    22981 gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat ggtgtttcct
    23041 caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc tctacgttgc
    23101 gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc accacaatca
    23161 actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg ttcagttggg
    23221 tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa acttgccagt
    23281 tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc tgcacatgct
    23341 ccacatccgt tgcaaagttg gctattaagt tgcggctggt agatcccgga gagcgtcggg
    23401 cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc aactgactga
    23461 tacgccaggc acgcgtcccc aatggtgaac tgcaaatccc aggccctggt gtggcgcgga
    23521 gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca ctcgttattt
    23581 ttgaaattaa cgctcggata gccgcccgcg ccgcgttgca gaatgttgtt ttcacaagca
    23641 ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc accggaccag
    23701 ggcggacgga taccgttact ggctttgcgc cagcgaccag tgagtatgcc ccgacgggat
    23761 gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga gagcgaccaa
    23821 ataaaccgca taattaataa gccattttta tagccgctaa gatattaaag gatgtgtcaa
    23881 agatgcatac cccgatcgag gtaaaacctg tagcaggatc aaaagagtgg cgggaagcgt
    23941 ggcaaaaacg ggctt
//
LOCUS       DAZUCE010000061        23619 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZUCE010000061 DAZUCE010000000
VERSION     DAZUCE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.061"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(20..265)
                     /gene="dinI"
                     /locus_tag="ACHY03_002000"
     CDS             complement(20..265)
                     /gene="dinI"
                     /locus_tag="ACHY03_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFS3905234.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            complement(339..1385)
                     /gene="pyrC"
                     /locus_tag="ACHY03_002001"
     CDS             complement(339..1385)
                     /gene="pyrC"
                     /locus_tag="ACHY03_002001"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFS3905235.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHTRHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            complement(1491..2051)
                     /locus_tag="ACHY03_002002"
     CDS             complement(1491..2051)
                     /locus_tag="ACHY03_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS3905236.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            complement(2185..2832)
                     /gene="grxB"
                     /locus_tag="ACHY03_002003"
     CDS             complement(2185..2832)
                     /gene="grxB"
                     /locus_tag="ACHY03_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFS3905237.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            complement(2896..4104)
                     /gene="mdtH"
                     /locus_tag="ACHY03_002004"
                     /pseudo
     CDS             complement(2896..4104)
                     /gene="mdtH"
                     /locus_tag="ACHY03_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
     gene            4340..4924
                     /gene="rimJ"
                     /locus_tag="ACHY03_002005"
     CDS             4340..4924
                     /gene="rimJ"
                     /locus_tag="ACHY03_002005"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFS3905238.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            4935..5582
                     /locus_tag="ACHY03_002006"
     CDS             4935..5582
                     /locus_tag="ACHY03_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFS3905239.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPSVTDMSSAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            5584..6507
                     /locus_tag="ACHY03_002007"
     CDS             5584..6507
                     /locus_tag="ACHY03_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS3905240.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            6617..8152
                     /gene="murJ"
                     /locus_tag="ACHY03_002008"
     CDS             6617..8152
                     /gene="murJ"
                     /locus_tag="ACHY03_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFS3905241.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(8192..8608)
                     /gene="flgN"
                     /locus_tag="ACHY03_002009"
     CDS             complement(8192..8608)
                     /gene="flgN"
                     /locus_tag="ACHY03_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFS3905242.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDVSQRWQEIAVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(8613..8906)
                     /gene="flgM"
                     /locus_tag="ACHY03_002010"
     CDS             complement(8613..8906)
                     /gene="flgM"
                     /locus_tag="ACHY03_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFS3905243.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            complement(8982..9641)
                     /gene="flgA"
                     /locus_tag="ACHY03_002011"
     CDS             complement(8982..9641)
                     /gene="flgA"
                     /locus_tag="ACHY03_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFS3905244.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            9796..10212
                     /gene="flgB"
                     /locus_tag="ACHY03_002012"
     CDS             9796..10212
                     /gene="flgB"
                     /locus_tag="ACHY03_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFS3905245.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTTPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            10216..10620
                     /gene="flgC"
                     /locus_tag="ACHY03_002013"
     CDS             10216..10620
                     /gene="flgC"
                     /locus_tag="ACHY03_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFS3905246.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVAGVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            10632..11327
                     /gene="flgD"
                     /locus_tag="ACHY03_002014"
     CDS             10632..11327
                     /gene="flgD"
                     /locus_tag="ACHY03_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFS3905247.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            11352..12557
                     /gene="flgE"
                     /locus_tag="ACHY03_002015"
     CDS             11352..12557
                     /gene="flgE"
                     /locus_tag="ACHY03_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFS3905248.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTADHAMTLVFNANGVLTSNPTENITTGAINGAEPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            12577..13332
                     /gene="flgF"
                     /locus_tag="ACHY03_002016"
     CDS             12577..13332
                     /gene="flgF"
                     /locus_tag="ACHY03_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFS3905249.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            13504..14286
                     /gene="flgG"
                     /locus_tag="ACHY03_002017"
     CDS             13504..14286
                     /gene="flgG"
                     /locus_tag="ACHY03_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFS3905250.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            14339..15037
                     /gene="flgH"
                     /locus_tag="ACHY03_002018"
     CDS             14339..15037
                     /gene="flgH"
                     /locus_tag="ACHY03_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFS3905251.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            15049..16146
                     /gene="flgI"
                     /locus_tag="ACHY03_002019"
     CDS             15049..16146
                     /gene="flgI"
                     /locus_tag="ACHY03_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFS3905252.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVILDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            16146..17087
                     /gene="flgJ"
                     /locus_tag="ACHY03_002020"
     CDS             16146..17087
                     /gene="flgJ"
                     /locus_tag="ACHY03_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFS3905253.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            17153..18796
                     /gene="flgK"
                     /locus_tag="ACHY03_002021"
     CDS             17153..18796
                     /gene="flgK"
                     /locus_tag="ACHY03_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFS3905254.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFSIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            18808..19761
                     /gene="flgL"
                     /locus_tag="ACHY03_002022"
     CDS             18808..19761
                     /gene="flgL"
                     /locus_tag="ACHY03_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFS3905255.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(19957..23139)
                     /gene="rne"
                     /locus_tag="ACHY03_002023"
     CDS             complement(19957..23139)
                     /gene="rne"
                     /locus_tag="ACHY03_002023"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFS3905256.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQAAPKVEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERAEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQTQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNSDNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAELAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQRP
                     TFAFEGKGAAGGHTATHHASAAPARPQPVE"
BASE COUNT         5597 a         6059 c         6364 g         5599 t
ORIGIN      
        1 aaacaaaaat cactgcatat tattcgctga caaaccagtc atcggcgctt tcccacgttt
       61 cctggagaat ttcgctaatg cgctgtttat cttcttttgt cgcgccaata accgataaat
      121 tattcgctgc ggcataacgt accgatacgt ggccttcatt atcaggaaac gcatactgaa
      181 tacggcggga aagttcgcca gccagggcgt caatagcccc agctggcaat ggagaagttt
      241 tcgctatggt gacttcaatt cgcataatag ccccctgttg aatgtactgg ttatttatac
      301 aggtaaaata acctaatgac aacaggaagc tacgattttt attgtttaac ggaccagcgt
      361 accgtttccc cggcgaggaa tggcaccagg gtgtcatcag tcagtgcgat gctttcagca
      421 acctgatgct cttcacgtac caattcgatg aatgtgtcgt tgaccggcaa cccatagaac
      481 tgcgggccgt ttacagaaca gaatgcttca aagtgctgca gagcattcat ctcttcaaag
      541 acggtagcgt aactgcccag cgcggttggg gcgttgaagc agcccgcgca gccgcagctg
      601 ctctctttgc gatgacgtgt atgtggcgca gaatccgtac cgaggaatac tcgattaaaa
      661 ccgctggcga ccagttcacg cagtgcctgt tggtgaatat tgcgtttgag gatgggtaga
      721 caatacaggt gcggacgcac gccaccaacc agcatatggt tgcggttaaa catcagatgt
      781 tgcggagtga tggtagcagc cagccgttca tttccgtcac ggacatagtc ggctgcgtct
      841 ttggtggtga tgtgttcaaa aacgactttc agtgcagtca ggcgctggcg cagcggttcc
      901 atcacgcttt ctataaagcg cgcttcacga tcaaaaatgt cgatatctgc atgtgtcact
      961 tcaccatgca ccagtagcgg cataccgatt ttttccatgc gctcaagtac cggcatgatt
     1021 gcgtcaattg acgtcacgcc gtggctggag ttagtggttg cgtttgccgg gtaaagtttt
     1081 gcagcggtga acacgccttc gttaaatccg cgctccagct cattaggatc cagcgaatct
     1141 gttaaataac aggtcatcaa tggggtgaaa tcgtgcccgg caggtacggc gtcaagaata
     1201 cgttggcgat acgccacggc agcctcaacg gtggtcacgg gcggagccag attgggcatt
     1261 acgatagccc gtccataaat ttcgctggta tacggcacga cagtttttaa catgtcgcca
     1321 tcgcggaggt gaaggtgcca gtcgtctggg cggcggatct ttaatacctg ggatggtgca
     1381 gtcattaatc tctatgctcc ggctgaaggg atgtttttgc cggacacaaa ggataagcgg
     1441 aaacgttttc ctttgcacga aaaataaagg gcgcgaatgc gccctcgtga ttaatcagta
     1501 aatggaatga caatttcgcc tggcttcact tcaatgcctt tcgccagttt tttcgccatt
     1561 gcttcgccct ggctgccatc ttcgcgcagg acgtaagcag gttgctggtt aaagtaattg
     1621 cgtaatgcct ggttcagata gggaagcaac gtttgcatca ccgtttgcat tttttccggt
     1681 tgtaccgtcg catcgaccac ctccatctct ttcaggaaga tcgcgccttt ttctttatca
     1741 aacacgggca gcgctttcag cttcagtttc atggtcgctt tctgactacc gaacagggag
     1801 ttcatgtcca gattggcgtc tccggttagg gtaaccttat tcggctcttc gcgaccaatt
     1861 tggctggtca ggtttgtcaa aacaatatgg gcgtcagcca cgccgggtaa accgatatct
     1921 tttgagaaat tattatgttt cgcaagcgac tggttaattt cttgttcggt gatggtgtat
     1981 tgggtgagtt gattacagcc aacgagcagg ccactgacga tcaatgcagc ggcaaataaa
     2041 aacttgttca tggtagtcct cgacatgaaa tctgcgtcaa tatcctgaca caacgcagca
     2101 tgtgtcacca gcgataaact cgccagcaga aaaaactgaa aacggcggca acccgcgaat
     2161 acaggctgcc gcggcgggtc aggattaaat cgccattgat gataacaaat tgatttgtgt
     2221 ctgtttcgcc atattatcgc ggtaatcagc aacgcggctt ggccagttaa ttccggctac
     2281 cagcgtcaga ttacgcagta gcgggaatag ctgaatatca tcttccgaaa gttcgccatt
     2341 cacggcgttc ggtttgacga tcagtttgtc cagcgcacgt aaatcatcgc tgatattctt
     2401 aatcagaccg tcagagtggg ccagcaggtc ggcaaaatta cccgcgctgg cctctttctt
     2461 gtcgacgaaa tatttgcgcg cggcgggagt agaaaactca tcaaatgccg atttggcaaa
     2521 acgcggcaac agcagtttgt tggcgtagcc attgaccttg cgcagccact cttcaatggc
     2581 aggggaacgt ttgccggtca gtaacggttt gccgtcgagt ttatcgacat agtgaacgat
     2641 gtccatgctt tctggcatat agcggctgtc atctttttgc agaatgggaa cctgtttttg
     2701 accgaccatc cgggtgggtg tttctgcgtc gtcgttgagc agaacatgta attcgacggg
     2761 gatatttttc aggccgaaaa tcatgcgggc tttgaggcag taagggcagt gatcgtaaat
     2821 gtatagcttc acgtgactcc tccatttggc tgtcagttca ctttcagtat ggaggagtca
     2881 ggggggagta tcaaatcagg cgtcgcgttc cagcaaacga cgcgcggcgc gtttctggct
     2941 aaactgccag cccagcgcaa ggaaagtgaa gatgccaata atgcccagca tcatccacgg
     3001 aagctctggt tggtgcgccg atttgcccag gtcaaacagc cagccgccac cgatataacc
     3061 aatagcgcca ccaatcgcca gacccagacg gctaaacccc atatagctgc cgcgagctct
     3121 tgcgtccgcc agcgaagcac ttaaggtttc acgcgcaggc tcggcaatga tcgacccgat
     3181 ataaaacaga caaatcaggg tgaaaagttg ttgcaggccg ctgaccatgc ccaccggcat
     3241 catgcttaat gacattatca acagcccagc catcaaccgg tgttccagac gaaaatgctt
     3301 ttcactccag cgggcgatag ggtagagcaa cgttaacgac agacacgctt caatggcata
     3361 catccattta acggcagagg gcgcgcccgc cacgtcgttg accataattg gcagcatcag
     3421 catcacttga acagccagca tgtagtaacc cgccagcgtc aggacatagg tgacaaaacg
     3481 cttgtcgcgc atcacgcggg tcatgccttc gcgaacgggc gtgcgtacgg tggagagttt
     3541 ccatgccggt aataaccacg cattgaacgc tgcacacagt acaaacaaaa ctgcccctgt
     3601 ggcgcagacc aggcgaaagt cgtattgcaa cagccagctc cccaacaatg cgccaatgac
     3661 cgcaccggca ctgtcctgca tcatcaacag cgagaaaaaa cgaccacgtt gttgtggacg
     3721 gattagtttc accaccagcg ccgaacgcgg cggatcaaac aacgtgccgc cgagtcccga
     3781 gagcaggcat gaaaaccaca atagccacgg ttcgtgggcg atacccattg tggcgaatcc
     3841 ggcggcgcgc atcagcatac cggtaacaat catcggtttg gcaccaaagc ggtcggcaat
     3901 tgcgccgccg aaaataccca gaccttgctg aataaactgg cgtagaccga gagcaatacc
     3961 gaccatgacg gcggctcagc ccatttgatc aacgaagcgg atagagatca gcgggaagac
     4021 aacaaagaac cccagcacga ccagcatatt atcgatgagc aggaaatatt tacccaggtt
     4081 cctcgcctgc gacacgcggg acatttcccc tcccgggaaa taaaagatga gcactttcta
     4141 ttctgcattg ccgctaagac ttttcccatc acttcgggga caatattttt ttatcaaaac
     4201 gtcgctttga tagagagttt ttatcaaata tgtgaataat gcagaaaatg gcattttgga
     4261 cttttcacag ggtctggttg cgcaggtata gtaatactta cagcgtatta aagacgttac
     4321 gggaaggagt aggtatagaa tgtttggcta tcgcagtaac gtgccaaaag tgcgcttaac
     4381 cacagaccga ctggtcgtgc gtctggtgca tgatcgtgat gcctggcgtc ttgcggatta
     4441 ttacgcagag aatcgccatt tcctcaagcc ctgggagcca gtgcgcgacg aaagccactg
     4501 ttatccatca ggctggcagg ccaggctggg gatgattaac gaatttcata aacaaggttc
     4561 agctttctac tttggcttat tcgacccgga cgaaaaagag attattggcg ttgccaattt
     4621 ttccaatgtt gttcgcggct cttttcatgc ctgctatctc ggttattcga ttgggcaaaa
     4681 atggcagggc aaagggctca tgtttgaagc cctgaccgca gccattcgtt atatgcagcg
     4741 cacccaacat attcatcgca ttatggctaa ttatatgccg cacaataaac gcagcggtga
     4801 tttactggcg cgactgggtt ttgaaaaaga aggctatgcg aaagactatc tgttgattga
     4861 tggacaatgg cgcgatcacg tactgacggc attaactacc ccagactgga cgcccggccg
     4921 ctaaggagaa tctcatgaaa tatcaactta ctgctctgga agcccgcgtg attggctgct
     4981 tgctggaaaa gcaggtgacc acgccggagc aatatccgct ctcagtcaat ggtgtagtaa
     5041 cggcctgtaa tcagaaaacg aaccgtgaac cggtcatgaa tctgagtgaa tctgaagtgc
     5101 aggaacagct ggataatctg gtcaaacgtc attatctgcg cacggtgagt ggctttggta
     5161 atcgagtcac caaatatgag caacgttttt gtaattcaga atttggcgat ctgaaactga
     5221 gcgccgcaga agtggcgtta atcaccacgt tgttattgcg tggtgcccag acgccgggtg
     5281 aactgcgcag ccgcgccgcg cgaatgtatg aattcagcga tatggcggaa gtggagttga
     5341 cgctggaaca actggcgaat cgcgaagatg gtccttttgt ggtgcgtctg gcccgcgaac
     5401 cgggtaaacg cgaaagccgc tacatgcatc ttttcagcgg tgaggtagag gatcagccgt
     5461 cggtaacgga tatgtcgagc gcggttgacg gtgatttaca ggcccgcgtc gaagccctgg
     5521 aaatcgaagt ggcagaactg aaacagcgtc ttgattcgtt gctggcccac ctgggagatt
     5581 aaagtgaaaa aattacgtat cggcgtagtg ggattaggcg gcattgcgca aaaagcgtgg
     5641 ttaccggtgc tggcggcagc gtctgactgg atgttacaag gagcctggtc gcctacgcgc
     5701 gcgaaagccc tgccaatttg tgaaagctgg cgcattcctt atgccgattc gttatccagc
     5761 cttgctgcca gttgcgatgc ggtttttgta cattccagca ccgccagtca ctttgacgtg
     5821 gtcagtacgt tactcaatgc gggggtacat gtctgtgtcg ataaaccgct ggcagaaaat
     5881 ctgcgcgatg ctgaacggct ggtggaactg gcggcgcgga aaaaactgac gttgatggtc
     5941 ggttttaacc gtcgtttcgc accactctac ggtgagttaa aaacgcaact cgccaccgca
     6001 gcctcgctaa gaatggataa acatcgtagc aatagcgtcg ggccacacga tctttatttc
     6061 acgttgctgg atgattatct gcatgtggtg gataccgcgc tgtggttgtc gggcggcaaa
     6121 gcctctctgg atggcggtac gctactgact aacgatgctg gcgaaatgct gtttgccgag
     6181 caccattttt cggccggtcc tttgcagatc accacttgta tgcatcgccg tgccggaagt
     6241 cagcgtgaaa ccgtgcaggc cgtgactgac ggggcgctca tcgacattac ggaaatgcgc
     6301 gaatggcgtg aggagcgtgg gcagggtgta gtgaataaac cgattcctgg ttggcagagc
     6361 acgcttgagc aacgtggatt tgtcggctgt gcgcggcact tcattgaatg tgtgcaaaat
     6421 cagacagttc cgcaaaccgc cggcgaacag gcagtgctgg cgcaacgtat cgttgacaag
     6481 atctggcgcg atgcgatgag tgaataaacc ctgtaacatc tggcggtagc aattcaccat
     6541 aatccaggta gactattcgc ctctttcagc gcctgccttg caggcgtttt gcccgtgggt
     6601 ctggattaga acaccgatga atttattaaa atcgctggcc gccgtcagct cgatgaccat
     6661 gttttcgcgt gtgcttggct tcgcacgtga cgcaattgtc gccagaatct ttggcgcagg
     6721 gatggcaacc gacgcctttt tcgtcgcttt taaacttcct aacttgttac gccgtatctt
     6781 tgccgaaggg gcattttccc aggcatttgt accgattctg gcggaatata aaagtaagca
     6841 gggtgaagac gccacgcggg tctttgtctc ttatgtttct ggcctgctga cacttgcgct
     6901 ggcggttgtg acggtcgctg gcatgctcgc cgcaccgtgg gtgatcatgg tgaccgcgcc
     6961 aggcttcgct gacacagctg acaaatttgc cctgaccagc cagctactaa agattacctt
     7021 tccctatatc ttgctgatct ccctggcgtc gctggtggga gcgattctga atacgtggaa
     7081 ccgcttctcg attccggcgt ttgctccaac actgcttaac atcagcatga ttggtttcgc
     7141 gctgtttgcc gcaccgtact ttaacccacc ggtgctggcg ctggcgtggg ctgttacggt
     7201 cggcggcgtc ctgcagctgg tgtatcagct accgcatctg aagaagatcg gcatgctggt
     7261 tttgccacgc attaacttcc acgatgccgg agcaatgcgc gtggtgaaac agatgggacc
     7321 ggcaatcctt ggcgtctctg tgagccagat ctccttaatc atcaacacca tttttgcctc
     7381 gtttcttgct tccggttcgg tgtcatggat gtattacgcc gaccgcttaa tggagtttcc
     7441 gtccggtgtg ctgggcgtgg cgcttggcac cattttgcta ccgtcgctgt cgaaaagttt
     7501 tgccagtggt aatcatgatg aatacaaccg tttgatggac tggggactgc gtctttgttt
     7561 cctgttggcg ctgccgagtg cggttgcgtt gggcattctt tccggtccgt taaccgtttc
     7621 gctgttccag tacggtaaat ttaccgcctt tgatgcgctg atgacccagc gggcgttaat
     7681 tgcctactcg gtgggtttga ttggcctgat tgtagtgaaa gtgttggctc ctggctttta
     7741 ttcccgccag gacattaaaa caccagtgaa aattgccatc gttacgctga ttttaacgca
     7801 attgatgaac ctggcgttta tcggtccgtt gaaacatgcc gggctgtcac tttctattgg
     7861 tctggcggcg tgtctgaatg cttcgctgct ttactggcag ttgcgtaagc agaaaatctt
     7921 taccccgcaa cctggctgga tggcgtttct gttgcgtctg gtggtggcgg tactggtgat
     7981 gtctggcgtg cttttaggta tgttacatat catgccggag tggtcattgg gtactatgcc
     8041 ctggcgttta ctgcgtttaa tggcggtcgt gctggcgggg attgccgcgt acttcgctgc
     8101 actggcggta ctgggcttca aagttaaaga atttgctcgc cggacggtgt aacaatgcat
     8161 tccggcctgc agtgcaggcc ggagataatc ttcagatcga aatcttttta ctgcctcgat
     8221 gggttgttga ggtctgaccg ttcgccccat atagcgtcgg ttcctgatgc ggtttcaaca
     8281 tttccagcgc ctgttgattg cgctcaatct gtccttccag taaccagccg ttatgttgat
     8341 tcatttggcg tagttgctgc gttttcacag caatttcctg ccagcgttga ctaacatcaa
     8401 cgctatttgc tgtattgggt tctttcctgc gtaactgttc gaggtaatcc aacgttgcca
     8461 gcagtgagct tttttgttct gtaatccatt gcaactggct gccgttgatc tgccccatag
     8521 agagatgttg ctgctcttga tccattaccg ttttgagatc gttaagcaca gcggacatct
     8581 ggtcgaggat ctctgcaaga cgcgtcatac gattaattac tctgcaagtc ttgctgcgct
     8641 tcgttgatca gcgcatcggc aattttgccg gtgtccattt ttagttcacc gttacgaatc
     8701 gccagtttta acgcttcgac acgttcaaga ttgatatcac tgctgccggg ttgcatcagt
     8761 tttgcttgtg cgtcgcttaa cgtcacactg gtgctggtgg aggctgttgt ttttgccgcc
     8821 cgggtgttcg ttaccggcgc gtcagtggtt tcgcgcggtt gaacggtgct tacgggcttc
     8881 agaggcgaag tgcgatcaat actcatggtt tattcctcat tgagggcgct tttatcatgt
     8941 gttgcttatt tatcggcaag ggacgggtaa tctttaacag cttacaggtt tataagaata
     9001 ttcccatctg catcaacaac gccgctgact acctgtccag ataccatgcg cacccgcgca
     9061 ttctgtgcga cggctgcatt gttcagcgcc tgaccttcag cattggcgct aaacccatca
     9121 ccgctggcga tcacgttgac gcgttgtccc gcttttaccc gccatgcctg gcgaaactgg
     9181 gttaactgga taggttgatc gggtgataga tcgcgcaggc taacggcatc aacaagttga
     9241 ttgatatcca gcaccgtacg cggtggcaag gtatccagcc gtccgcgttt cagtttgaca
     9301 ttgccagctt ccagctttcc tccacgtaca atgggcatcg cggcaaccac ataatttcct
     9361 gtggcctgca cattaacctg taaatatcgt ttgtcgttac cgcagcgtgc caacacattc
     9421 acattgcccc acaggcggga attattgctc atcgaaagca atggctgctc gcacggcggt
     9481 agcagattgg gtgctgtacg aatagaaaca cgaacctcat cacttacccc cgcgagttgg
     9541 gcgctaaaaa agttgtgcaa ttgcgatgtg agattgctcg ccgtacttaa cggactgaac
     9601 agtatcgcga tgatcgccac gctacgtttt attgtcagca ttttcgcccc cagccatttc
     9661 tacaacgtga attgtacctg tccgcaatga ccatcaacgg cataaatagc gacccatttt
     9721 gcgtttattc cgccgataac gcgcgcgtaa aggcatttaa gctgatggca gaattttgat
     9781 acctgcggag gagatatgct cgataagctc gatgccgcct tacgttttca acaagaggcg
     9841 ctcaatctgc gcgcccagcg tcaggaagtg ctggcagcaa acatcgccaa tgccgatacc
     9901 cctggttatc aggcgcgcga tatcgatttt gccagtgaac ttaaaaaagt catgcaacgt
     9961 ggacgggatg caaccagtgt ggttgcactg acgatgacct caacgcaaca cattccggcg
    10021 caggcgctga cgactcctac cgcagaactg caataccgta ttccggacca gccttcgctt
    10081 gacggtaata ccgtcgatat ggatcgcgaa cgcacccagt ttgccgataa cagcctgcaa
    10141 taccagatga gccttagtgc gttgagcggg caaatcaaag gcatgatgaa cgttttacag
    10201 agcggaaatt aacggatggc actgctgaat attttcgata tcgccgggtc ggcgttaact
    10261 gcccagtccc agcgcctgaa cgtggcggcc agtaatctgg cgaatgctga tagcgtgacc
    10321 ggtcccgatg gacagccata tcgggcaaaa caggtggtat tccaggttaa cgctgcacca
    10381 ggtgctgcga caggcggcgt aaaggttgcc ggtgttatag aaagtcaggc cccggacaaa
    10441 ctggtttatg aaccgggtaa tccgctggcg gatgcaaagg gctacgtaaa aatgccgaac
    10501 gttgatgttg tcggagagat ggttaacacc atgtcggcgt cacgcagcta tcaggccaat
    10561 gttgaagtgc tcaacacggt gaaaagcatg atgctgaaaa cccttacgct cggtcaataa
    10621 aggagaaagc tatgtccatt gcggtaacca ccaccgatcc gacaaatacc ggcgtcagta
    10681 ccaccagcag tagttcgctc acgggcagca acgccgcaga tttacaaagc agttttctga
    10741 ctttgctggt ggcgcagctg aaaaaccagg acccgaccaa tccaatggaa aacaacgagc
    10801 tgacgtcgca attggcacaa atcagcacgg tcagcgggat tgaaaaactc aataccacgc
    10861 tcggatctat ttccggacag attgataaca gccagtcgtt acaggccagt aacctgatcg
    10921 gtcacggcgt gatgatcccc ggcaccactg ttcttgcggg aaccggcagt gaagaagggg
    10981 ctgtgaccac gaccacgccg tttggtgttg agctgcaaca ggcggcagac aaagttacgg
    11041 ccaccatcac cgataaaaat ggcgcggttg tgcgcaccat tgatattggt gaactgaccg
    11101 ccggagtcca cagtttcacc tgggacggta cgttgactga cggcagcact gcgccgaacg
    11161 gttcttacaa tgtagcgatt agcgccagta acggtggtac acaactggtt gcccagccgc
    11221 tgcagtttgc tctggtgcag ggtgtgatcc gcggcaacaa cggtaatacg ctggatctcg
    11281 gcacttacgg caccaccacc ctcgacgaag tacggcagat aatttaagcc ttcacatttc
    11341 aggagtcagt catggccttt tctcaagcgg ttagcggatt aaacgctgcc gccaccaacc
    11401 tcgatgttat tggcaacaat atcgccaact ccgccacata cggctttaaa tcaggcacgg
    11461 cctcttttgc cgatatgttt gccggttcga aagtgggact gggggtaaaa gttgccggta
    11521 tcactcagga ctttaccgat ggcacgacca ccaacaccgg gcgtggtctg gacgttgcta
    11581 tcagccagaa cggttttttc cgtctggtag acagcaacgg ttcggtgttc tacagccgta
    11641 acggacaatt taagctggat gaaaaccgta acctggtgaa tatgcaaggt ttacagctga
    11701 cgggttaccc ggcaaccggt acgccgccga ctattcagca aggggcgaat ccgactaaca
    11761 tttcgatccc gaataccctg atggcagcga aaactaccac cacggcgtcg atgcagatca
    11821 acctgaattc cagcgatccg cttccctctg ttaacgcatt tgatgccagc aatgcggata
    11881 gctataacaa aaaaggttcg gtgactgttt tcgacagtca gggtaatgct catgacatga
    11941 gcgtctactt tgtgaagacc ggggataata actggcaggt ctacacccag gatagcagtg
    12001 atccaacagg cacagctgac catgcaatga cgctggtgtt taatgccaat ggcgttctga
    12061 cgtcaaatcc aacagagaat attacaactg gcgcaattaa cggcgcagaa cccgccacgt
    12121 ttagcctgag cttcctcaac tccatgcagc aaaataccgg cgctaacaac attgtggcaa
    12181 ccacccagaa tggctacaaa ccgggcgatc tggtgagtta tcaaatcaat gatgacggta
    12241 cggttgtcgg caactattcc aacgaacaaa cccaactgct ggggcagatt gtactggcga
    12301 actttgccaa caacgaaggt ctggcatccg aaggcgacaa cgtctggtct gcgacgcaat
    12361 cttctggcgt ggcgctgttg gggacagccg ggacgggcaa ctttggcacc ctgaccaacg
    12421 gtgcgttgga agcgtccaac gtcgatctca gtaaagaact tgtcaatatg atcgttgccc
    12481 agcgtaacta tcagtctaac gcccagacca tcaaaaccca ggaccagatc ctcaacacgc
    12541 tggttaactt acgctaatcg ctgacgggat agctcaatgg atcacgcaat ttataccgcg
    12601 atgggagcag ccagccagac gctgaatcaa caggcggtaa ccgccagtaa tctggccaat
    12661 gcctctacac ccggttttcg cgcgcagtta aacgccttgc gcgcagtgcc ggtggaaggg
    12721 ctttcactgc ccacgcgcac gctggtaacg gcgtcaacgc cgggtgcaga catgacgccc
    12781 ggcaagatgg attacacctc gcgcccgctg gacgttgcgt tgcagcagga tggctggctg
    12841 gccgtgcaga ccgctgacgg cagcgaaggg tatacgcgta atggcagcat tcaggttgat
    12901 cccaccgggc aactgacaat tcaggggcat ccggtgatag gcgaggcggg gccaattgct
    12961 gtgccggaag gggcggaaat cacgattgct gccgatggca caatctcggc gctcaatccg
    13021 ggcgatccgg caaatacggt tgcgccagtg gggcgtctga agctggtgaa agccacgggc
    13081 agcgaagtgc agcgcggtga cgacggcatt tttcgtttaa gcgcagaaag ccaggctaca
    13141 cgtggaccgg tactgcaggc agatccaacc ttgcgtgtga tgtcgggtgt tctggaaggc
    13201 agtaacgtca atgccgttgc ggcaatgagc gacatgattg ccagcgcgcg gcgttttgaa
    13261 atgcagatga aggtgatcag cagcgtcgat gataacgcag gccgtgccaa ccaactgctg
    13321 tcgatgagtt aattgaaagg atacatgaca agtataagtt gcccgatgcg caagtttatc
    13381 gggtctatgg gggcaatcgc aatttatcga ttttgcgagc acttgtaggc cggataaggc
    13441 gtttacgccg catccggcaa gaagacatat gcactttgtc actaatccac tacaggacat
    13501 tttatgatca gttcattatg gatcgccaaa acgggccttg acgcccagca aaccaatatg
    13561 gacgtcattg ccaacaacct ggcaaacgtc agtaccaacg gttttaagcg tcagcgtgcg
    13621 gtgtttgaag atctgcttta tcaaaccatt cgccagccgg gggcacagtc ttccgaacaa
    13681 accaccttac cctccggatt acaaatcggc acgggggtac gtccggtcgc cacggaacgc
    13741 ttacacagcc agggcaacct gtcgcaaacc aataacagta aagatgtcgc gattaaaggg
    13801 cagggctttt tccaggtgat gttgccagat ggctcatcag cctatacccg tgacggctct
    13861 ttccaggtgg atcagaacgg gcagctggtg acggctggtg gttttcaggt gcagccagcg
    13921 atcaccattc cggcgaatgc gttaagtatc accatcggtc gtgatggcgt ggtcagcgta
    13981 acccaacaag gccaggcagc tccggttcag gttgggcagc ttaatctcac cacctttatg
    14041 aatgacaccg ggctggagag cattggcgaa aacctctaca ccgaaacgca atcctccggt
    14101 gcaccgaacg aaagcacgcc gggcctgaac ggcgcgggac tgctgtatca agggtatgtt
    14161 gaaacgtcta acgtcaacgt ggcggaagaa ctggtcaata tgattcaggt gcaacgcgct
    14221 tacgaaatca acagtaaagc ggtgtccacc accgatcaga tgctgcaaaa actgacgcaa
    14281 ctctaaggca taaccggtgg caggttcacc ggtttattga tttttgaaga tgatagccat
    14341 gcaaaaaaac gctgcgcata cttatgccat ttccagcttg ttggtgcttt cactaaccgg
    14401 ctgcgcctgg ataccctcca cgccgctggt acagggagcg accagtgcac aaccggttcc
    14461 cggtccgact cccgtcgcca acggttctat tttccagtct gctcagccga ttaactatgg
    14521 ctatcaaccg ctgtttgaag atcgtcgacc acgcaatatt ggcgatacgc tgaccatcgt
    14581 gttgcaggag aacgtcagcg ccagcaaaag ctcctctgcg aatgccagcc gtgacggtaa
    14641 aactaatttt ggctttgata ctgtgccgcg ctatttgcag gggctgtttg gtaacgctcg
    14701 tgccgatgtc gaagcctccg gtggtaacac gttcaacgga aaaggcgggg ccaatgccag
    14761 caataccttt agcggcacgt tgacggtgac ggttgaccag gtactggtca acggcaacct
    14821 gcatgtggtg ggtgaaaaac agattgccat taatcagggt accgaattta ttcgcttctc
    14881 tggcgtggtt aatccacgca ccatcagcgg cagcaatacc gtaccgtcta ctcaggtggc
    14941 ggatgcgcgc attgaatacg taggcaatgg ctacattaac gaagcgcaaa atatgggctg
    15001 gttgcagcgt ttcttcctta acctgtcgcc aatgtaagtg aggctgtggt gattaaattt
    15061 ctctctgcat taattcttct actggttacg acggcggctc aggctgagcg tattcgcgat
    15121 ctcaccagtg ttcagggggt aaggcaaaac tcactgattg gctatggtct ggtggtgggg
    15181 ctggatggca ccggtgacca gacaacccag acgccgttta ccacacaaac gcttaataac
    15241 atgctctcac agctgggaat taccgttccg acgggcacca atatgcagct aaaaaacgtc
    15301 gctgcggtaa tggtgacagc gtcacttcct ccgtttggac gtcaggggca aaccatcgac
    15361 gtggtggttt cttccatggg taatgccaaa agcttgcgtg gaggtacgtt gttgatgaca
    15421 ccgcttaagg gcgttgacag tcaggtgtat gcgctggcac agggcaatat tctggttggc
    15481 ggcgcaggag cctccgctgg cggtagcagt gttcaggtga accaactgaa cggtggacgg
    15541 atcaccaatg gtgcggttat tgaacgtgaa ttacccagcc agtttggcgt cgggaatacc
    15601 cttaatttgc aacttaacga cgaagatttc agcatggcgc agcaaatcgc tgacaccatc
    15661 aaccgcgtgc gtggatatgg cagcgccacc gcgttagatg cgcggactat tcaggtgcgc
    15721 gtaccaagtg gcaacagttc ccaggtccgt ttccttgccg atatccagaa tatgcaggtt
    15781 aatgtcaccc cgcaggacgc taaagtagtg attaactcgc gcaccggttc ggtggtgatg
    15841 aatcgcgaag tgatcctcga cagctgcgcg gtagcgcagg ggaatctctc agttacagtc
    15901 aatcgtcagg ccaatgtcag ccaaccagat acaccgtttg gtggcggaca gaccgtggta
    15961 acgccacaaa cgcagatcga cttacgccag agcggcggtt cgctgcaaag cgtacgttcc
    16021 agcgccagcc tcaataacgt ggtgcgcgcg ctcaatgcgc tgggcgctac gccgatggat
    16081 ctgatgtcta ttttgcaatc aatgcaaagt gcgggatgtc tgcgggcaaa actggaaatc
    16141 atctgatgat cagcgacagc aaactactgg caagtgcggc ctgggatgcg caatcactca
    16201 acgaactaaa ggcgaaagcg ggcgaagatc cggcggcaaa tatccgtccg gtggcccgcc
    16261 aggtggaagg gatgttcgtg cagatgatgt tgaaaagcat gcgcgacgct ttaccaaaag
    16321 atggcctgtt cagcagcgag cacactcgcc tgtataccag tatgtatgac cagcagattg
    16381 cccaacagat gacggcgggc aaaggtctgg ggcttgcaga gatgatggtt aagcagatga
    16441 cgccagaaca accattgcca gaggagtcca cgccagcagc accgatgaaa ttcccgctcg
    16501 aaaccgtggt gcgttatcaa aatcaggcgc tttcgcagct ggtgcaaaag gccgtgccac
    16561 gtaactacga tgattcgctg ccgggtgaca gtaaagcatt cctcgcgcaa ctctcgctgc
    16621 ccgcccaact ggcaagccag caaagcggtg tgccacatca tttgatcctc gctcaggcgg
    16681 cgctggaatc tggttggggg caacggcaaa tccgccgcga aaacggcgag ccgagctata
    16741 acctgtttgg cgtcaaagcc tctggcaact ggaaagggcc agttactgaa atcaccacga
    16801 ctgaatatga aaatggcgaa gcgaagaaag taaaagcgaa atttcgggtc tacagctcgt
    16861 atctggaagc attgtcggat tacgttgggc ttttaacccg taacccgcgc tacgctgccg
    16921 tgacgaccgc cgcgagtgcg gaacaggggg cgcaggccct acaggacgcg ggctatgcca
    16981 ccgatcctca ctatgcccgt aaactcacca acatgattca gcagatgaaa tcgataagcg
    17041 acaaggtgag caaaacctac agcatgaaca ttgataatct gttctgaata actcaagtcc
    17101 ggcgggtcgc tgccgataat actctgtaat tgaaggctta taaggaacct ccatgtccag
    17161 cttgatcaat aacgccatga gcggactgaa cgcggcccag gcggcgttaa atacggcaag
    17221 taataatatc tccagctata acgttgccgg atatacccgc caaaccacta ttatggcgca
    17281 ggccaatagc acgttgggtg ctggcggctg ggttggcaat ggtgtctacg tttctggtgt
    17341 gcagcgcgag tatgatgcgt ttattaccaa ccagttacgt gcggcgcaga cgcaaagtag
    17401 cggcctgact gcccgctatg agcagatgtc gaaaatcgac aatatgctct ccaccagtac
    17461 ctcttcgctg gcaacacaga tgcaggattt cttcaccagc ctgcaaacgc tggtgagtaa
    17521 tgcggaagac ccggcagcgc gccaggcgct gattgggaaa tcagaagggt tggtgaatca
    17581 gtttaaaacc accgatcagt atcttcgcga ccaggacaaa caggtcaata tcgcgatagg
    17641 tgccagcgtt gatcagatca acaattacgc taaacaaatc gccagcctga acgatcaaat
    17701 ctcccgcctg acaggcgtgg gggcaggggc gtcacctaac aatctgctgg atcaacgcga
    17761 tcaactggtg agcgaattaa accagattgt tggtgtagaa gtcagcgttc aggatggcgg
    17821 cacttataac atcacgatgg ccaatggtta ctcactggtt cagggaagta cggctcggca
    17881 actggcggca gttccttcca gcgccgaccc ttctcgtacg actgtcgctt acgttgatgg
    17941 gacggcaggc aatattgaga tcccggagaa attgctgaat accgggtcgc tgggcggcat
    18001 tctgacattc cgttctcagg atctggacca gacgcgtaat acgcttggac aactggcgct
    18061 ggcatttgcc gaggctttca acacccaaca caaagccgga tttgatgcca acggcgatgc
    18121 cggtgaagat ttcttttcta tcggtaagcc cgcggttctg caaaacacga aaaacaaagg
    18181 tgacgttgcg atcggtgcca cggtaactga tgcctccgcg gtactggcga cagattacaa
    18241 aatctcgttc gataataatc agtggcaggt cacccgcctt gccagcaata ccacttttac
    18301 ggtgacgccg gatgccaacg gtaaagtggc atttgatggt ctggagttga cgtttacagg
    18361 aacgcctgcc gttaacgaca gcttcacgct gaaaccagta agtgacgcca tcgtcaacat
    18421 ggatgtatta atcaccgacg aagcgaaaat cgcgatggcg agcgaagaag atgcgggtga
    18481 tagcgacaac cgcaacggtc aggccctgct ggatctgcaa agcaacagta aaacggtggg
    18541 cggtgcgaaa tcctttaacg acgcttatgc ctcgttagtg agtgatatcg gtaataaaac
    18601 cgcgacgttg aaaaccagta gcaccacgca aggtaatgtg gtgacgcagc tttccaatca
    18661 gcagcagtcg atttccggtg tcaatctcga tgaggagtac ggaaatctgc aacgttttca
    18721 gcagtattac ctggcgaatg cgcaggttct gcagacggca aacgcgattt ttgatgcgct
    18781 gattaacatt cgctaagggg agataagatg cgtttcagta cccagatgat gtaccagcaa
    18841 aacatgcgtg gcatcaccaa ttctcaggcg gaatggatga agtacggcga gcagatgtcg
    18901 acaggtaagc gagtcgttaa cccttctgac gatcccattg cagcatcaca agccgtagtt
    18961 ctctcccagg cacaggcgca aaacagccag tacacgctgg cgcgtacttt cgccactcaa
    19021 aaagtgtcac tggaagagag tgtacttagc caggtcacca cagctatcca gaatgctcag
    19081 gaaaaaattg tctatgccag caatggcact ttgagtgacg atgaccgggc ctcgctggct
    19141 acggatattc aggggcttcg tgaccagttg ctgaatctgg cgaacaccac tgacggtaac
    19201 gggcgctaca tttttgccgg ttataaaaca gagactgcgc cgtttagtga ggctaacggt
    19261 gattatgtcg gcggaacaga aagtattaag caacaggtcg atgcttcgcg ttcgatggtg
    19321 ataggacata cgggcgacaa aattttcgac agtattacca gtaacgcggt agcggaacca
    19381 gacggtagcg cttctgaaac caatcttttt gccatgctgg atagcgccat cgcagccctg
    19441 aaaacgccgg tcgcggatag cgaagcggat aaagaaatcg ctgctgcggc gttagataaa
    19501 accaaccgcg gactgaaaaa ctcgctgaac aatgtcctga ccgttcgcgc ggaattaggc
    19561 acgcagctga acgaactgga gtcgctggat tcattaggta gcgatcgcgc tttagggcaa
    19621 acgcagcaga tgagcgatct ggttgatgtg gactggaatg caactatttc atcttacatc
    19681 atgcagcaaa cggcattgca ggcatcgtat aaagcattta ccgatatgca gggattgtcg
    19741 ctcttccagc tcaacaaata agctcgcttt aaaacatatc atgaaactgg gtatgttttg
    19801 tctgcccgct ctgggatcgc cggggcgggc atttttttgc ctattttgca ttgttggtta
    19861 gcaaggatgc catttgatga attttaatat gttgattcaa agatgaaata aaaaaagccc
    19921 tggcagttac cagggcttga ttacttgagc taattattac tcaacaggtt gcggacgcgc
    19981 aggagcggca gaggcatgat gcgttgccgt atgaccacct gcggcacctt taccttcgaa
    20041 ggcaaaagta gggcgctgcc agtcactgtg acgcggtgct tccggaacat attccggtgc
    20101 cggagcgcgc gtcatcggcg cggtagcgtg gttatgctca acggtgacct caggttcaac
    20161 cgcagctacc gtttcaactt ctgctgcgac ttcagcgacg actggcgcgg caggttgaac
    20221 aacttcaggt tcagcaacca caacctcagc agtttcggca acttcttcaa tatctgccgt
    20281 ctcttcctgc ggttcaacca ccggttctgc gagttctgca acttcctggg ctacggcaac
    20341 atcagactcg gtaatcacct gcggctgttc agttaccgcg gcggcaatta cttcaggatg
    20401 cgtcgtttca accacttccg gttgcggttc ggcaatctga acgggggctt ctacgacttc
    20461 agccatttct tcaacaactg gcgcgctaac aaccggttcg acagcggcgg cgacagggac
    20521 ctcagtcacc atcggctgca cttgtacttc ttcctgttcg cgctgctctt caacttgtac
    20581 atcttgcgga cgtacaattg gatagcggat ccagactttg ccagaggcca gttccggaga
    20641 cgcgcacgct acggtcaacg gcattggcga ctgggttgga taacgctcgt cacgatagcg
    20701 acgacgacgc tgaccactca cgcgcaggtg acgaggcgag cggcgagaac gacgcggcat
    20761 gccaccgttg tcacggttat cagaattgtt ctcttcttgc tgttctggtg cagtttgcgc
    20821 tacgactggc agcgggactt tcaccagttc tgtgcgtgga gctggcgctt cctgaacaat
    20881 tggttcggca gcggcagttt cttcaaccac cggtgcgact accgcttctt cggctacgct
    20941 ttgctcgtaa cgcactttct gattgagctg acgctgttta cgacgcggct gaaccggacg
    21001 tacacgttct tcctgttcgg tttcctgaac agattgctct tcaacattca gcgccttcgc
    21061 ttcttgttgc gtctgacgtt tatcatcgtt acggcggcgg ctacgttcac gacgcggcgc
    21121 ttgttgctcg tcggtggtac gtgctttttc cgttacctca gcctgctgac ggctctcacg
    21181 cgtctcggca gtctgctgct gtgcctggcg acgattacga cggttttctt cgcgattatc
    21241 gctgccttca gcacgttcac tacgggtgtc gcggcgctca ttacggtcac ggcggttgtt
    21301 ctgacgaggc ttgcgacgat cctgttgacg ttccggtttc gcttctactt tcggtgctgc
    21361 ttgctcggac ggtttggttt cttcaccacc gctgaacagc gctttcagtg cgccgaagaa
    21421 gcggctcaac agcccaggtt gtgcaggagc tgctggtgtt gccggtgcag ctttcggtgc
    21481 tggggctaca acaggtgcgg caggttcagc tggcgttggc gcaggcggca catccggcat
    21541 ggcaaaggtt gccagcgcag gttgttccgg acgcttacgt tcagcgaact cttcttcaga
    21601 cggcagcgcc atcgcttctt catgcagctt cggcagcatg tagcttaagg ttggggtttc
    21661 ttccccttta cgcacgcgca gcacgtggta gtgcggggtt tccatctgat cgtttggcac
    21721 aattacacag cgcacaccgt cctgacgagt ttcaatggca tttaccgcag aacgtttttc
    21781 attcagcagg taagaagcga ttggcacagg aacaatggcg tgaacttcct gggtgttctc
    21841 tttcagcgct tcttcttcga tcaaacgcag aatagagagc gacagcgatt cgttgtcacg
    21901 cacggtaccg gtaccggagc agcgcgggca gacgtgatgg ctggattcac ccagtgatgg
    21961 gctcaggcgc tgacgggaca tttccagcag gccaaagcga gaaatatggc tgatttgaat
    22021 acgcgcacgg tcctgacgca ccgcttcacg cagacggttt tctaccgcac gctggtgacg
    22081 tacaggcgtc atgtcgatga agtcgataac aatcagaccg ccgaggtcac gcaggcgcag
    22141 ctgacgagca atctcatcag cagcttcgag gttagtgtta aacgcggttt cttcgatatc
    22201 gccgccgcgg gtcgcgcgtg cggagttgat gtcgatggcc gttaacgctt cggtgctgtc
    22261 gataacaatg gaaccaccgg atggcagacg aacttcacgc tggaaggcgg actcgatctg
    22321 cgactcgatc tggtagtggc tgaacagcgg gatctcgccg gtgtacagtt tgattttgct
    22381 gctgaaatcc gggcgaccta atgcagcgat atgctgacgt gccagttcga gcactttcgg
    22441 gttatcgata aggatttcgc cgatgtcctg acgtaagtaa tcgcggaatg cgcgaacgat
    22501 tacgttgctc tcctgatgaa tcaggaacgg agccgggcgg ctttcagcgg cttttttgat
    22561 ggcttcccag tgtttcagac ggaagcttaa atcccattgc agcgcctcag cagatttgcc
    22621 gacgccagcg gtgcgcacga taagccccat gccttccggc agttcaaggc ttgccagtgc
    22681 ttcttttaat tcggtacggt cgtcgccttc gatacggcga gaaatgccac ccgcgcgcgg
    22741 gttgttcggc atcagaacca gatagctacc cgccagactg ataaaggtgg ttaatgccgc
    22801 gcctttgttg ccgcgctctt ctttatcgat ctgaacaatg acttcctgac cttcacgcaa
    22861 cacatcttta atgttgggac gaccatgagc actgtagtta gcagggaaat attcgcgggc
    22921 aatttctttt agtgggagga aaccgtgacg ttcagcgccg taatcaacaa aagcagcttc
    22981 cagactcggt tcaatgcggg tgattttacc tttgtagatg tttgcctttt tctgctcgtg
    23041 ccctggactt tcgatatcca ggtcatacag acgctgccca tctacaaggg caacgcgcaa
    23101 ctcttcctgc tgagttgcgt tgattaacat tcttttcatc gtaacttact cattattctt
    23161 acattgacga ctaagctgcg ggcaaagtaa cgcctttccg ggtgtgaacc gatggcctcg
    23221 tgtctagtcg cgtcgccaac ctcacggtta tcgtcagctc aaagaggcgc agagtgtcgg
    23281 ttgcccgttt ttcatgcgga aaaacagcgc aattatcaaa gaaacagact gggtattact
    23341 ctccagaaat atttccatct accggtaagg actgcaaccc gcagcccgct aactgcctga
    23401 aagatcaata cgtcttacgc cattgctgcg tggatgatcg gtcgggcaaa atgggttatt
    23461 ccgtaaaatt tcttgtttta acaaggatgg acacggaaac ggcctcatta ttccactgca
    23521 agccttgtta tagcaagatg acttttacca tttatcaccc gcttactcac agttttttca
    23581 cttcttgctg gtgattggtt taataaccat caataaagt
//
LOCUS       DAZUCE010000062        23283 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZUCE010000062 DAZUCE010000000
VERSION     DAZUCE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.062"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..868
                     /locus_tag="ACHY03_002024"
     CDS             <1..868
                     /locus_tag="ACHY03_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFS3905257.1"
                     /translation="FTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTTMPL
                     VTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYALSY
                     LPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGPPMV
                     IYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCISTMILGSL
                     VGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLAFGK
                     KVAAIRRAEESK"
     gene            977..3013
                     /gene="yihQ"
                     /locus_tag="ACHY03_002025"
     CDS             977..3013
                     /gene="yihQ"
                     /locus_tag="ACHY03_002025"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFS3905258.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFNRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYNGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKDVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKSI"
     gene            3059..4444
                     /locus_tag="ACHY03_002026"
     CDS             3059..4444
                     /locus_tag="ACHY03_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3905259.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPANPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            4484..5887
                     /locus_tag="ACHY03_002027"
     CDS             4484..5887
                     /locus_tag="ACHY03_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3905260.1"
                     /translation="MSDHNPLTLKLNLWEKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILIGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCI
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            5954..6646
                     /gene="ompL"
                     /locus_tag="ACHY03_002028"
     CDS             5954..6646
                     /gene="ompL"
                     /locus_tag="ACHY03_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFS3905261.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(6737..8002)
                     /locus_tag="ACHY03_002029"
     CDS             complement(6737..8002)
                     /locus_tag="ACHY03_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3905262.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(8104..9084)
                     /locus_tag="ACHY03_002030"
     CDS             complement(8104..9084)
                     /locus_tag="ACHY03_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFS3905263.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(9092..9802)
                     /locus_tag="ACHY03_002031"
     CDS             complement(9092..9802)
                     /locus_tag="ACHY03_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS3905264.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(10019..11842)
                     /gene="typA"
                     /locus_tag="ACHY03_002032"
     CDS             complement(10019..11842)
                     /gene="typA"
                     /locus_tag="ACHY03_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFS3905265.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            12215..13624
                     /gene="glnA"
                     /locus_tag="ACHY03_002033"
     CDS             12215..13624
                     /gene="glnA"
                     /locus_tag="ACHY03_002033"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFS3905266.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13798..14847
                     /gene="glnL"
                     /locus_tag="ACHY03_002034"
     CDS             13798..14847
                     /gene="glnL"
                     /locus_tag="ACHY03_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFS3905267.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            14859..16268
                     /gene="glnG"
                     /locus_tag="ACHY03_002035"
     CDS             14859..16268
                     /gene="glnG"
                     /locus_tag="ACHY03_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFS3905268.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            16380..16490
                     /locus_tag="ACHY03_002036"
     CDS             16380..16490
                     /locus_tag="ACHY03_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFS3905269.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(16680..18053)
                     /gene="hemN"
                     /locus_tag="ACHY03_002037"
     CDS             complement(16680..18053)
                     /gene="hemN"
                     /locus_tag="ACHY03_002037"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFS3905270.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(18242..18748)
                     /gene="yihI"
                     /locus_tag="ACHY03_002038"
     CDS             complement(18242..18748)
                     /gene="yihI"
                     /locus_tag="ACHY03_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFS3905271.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            complement(18867..19033)
                     /locus_tag="ACHY03_002039"
                     /pseudo
     CDS             complement(18867..19033)
                     /locus_tag="ACHY03_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19330..19962
                     /gene="yihA"
                     /locus_tag="ACHY03_002040"
     CDS             19330..19962
                     /gene="yihA"
                     /locus_tag="ACHY03_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFS3905272.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(20344..23130)
                     /gene="polA"
                     /locus_tag="ACHY03_002041"
     CDS             complement(20344..23130)
                     /gene="polA"
                     /locus_tag="ACHY03_002041"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFS3905273.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         5592 a         5925 c         5730 g         6036 t
ORIGIN      
        1 ttttacgtta ttttcaccgg atggggtcat gctggaaaca aacgtggtat gtatcgttat
       61 cgcgttaatg tttctttata cctttgtggg gatatgcgcg ggatttggcg gtggtttaac
      121 cactatgccg ctcgtcacgt taatgctccc cgtaaaaatg gcaacaccac tctcggtaat
      181 tgtcggcacc gcgaccgcct tgtacgccac ctggctgtca cgtaaagaaa cggattggcg
      241 atcggcaatg gtactgatta ttttctcgtt tctgggaata cccgtcggtt tatatgcgct
      301 gtcatattta cccgaccatt ttatgaaagt gggcttaggc ggattcctta tcctgtactc
      361 cttttacagt atgtttattc cacgcctgcc gatttatgac cgtagctgga tcgccgcacc
      421 tatcggtgca gtagcaggcg cattaggcgc agctttctca accaacggac caccgatggt
      481 tatctacggt atgctgcgta acttaggccc ggcggcattt cgtggcactc tgaacgcttt
      541 cttcaccgca aacaacatcg cggtgatcgg cgggctggcg accagcggaa ttttgactgt
      601 ttcgatcttt aaactggtgc tgttttgtat ttccaccatg atcctcggtt cactggttgg
      661 acaatatgtg cataaacgca tttcagtcaa agtgttccgt gtgatggtct ttttgttgct
      721 gattgcctcc ggttctatgt tgataaaagg cgcgctggga attcccgctg ctgccgcgct
      781 tctgcctcct tttaccttgt tagttatact acaactggca tttggtaaaa aagtggcggc
      841 aatccgccgg gctgaagaaa gtaaataatc acttatgcgg gttttaattt ataccagcac
      901 cacattcttt aattaatgaa acctgtaaca acaggatggc atgaaatatt agctttatac
      961 tctatggaga ttatttatgg atacgccacg cccacaatta ccagattttg aatttcatca
     1021 gaataacgac agttttaccc tacattttca acaacgtctt attttaaccc atagcaaaga
     1081 taatccttgt ttatggattg gctcaggtat agcggacatc gatatgttcc gcggcaattt
     1141 cagcattaaa gataaactac aggagaaaat tgcgcttacc gacgccatcg tcagccagtc
     1201 accggatggt tggttaatcc atttcaaccg tggttctgac attagcgcca cgctgaatat
     1261 ctctgccgac gatcagggcc gtttattgct ggaactacaa aacgacaacc ttaaccacaa
     1321 ccgcatctgg ctgcgccttg ccgctcaacc agaggaccat atctacggct gcggcgaaca
     1381 gttttcctac ttcgatctgc gcggcaaacc gttcccgcta tggaccagtg aacaaggcgt
     1441 tggtcgcaac aaacaaacct atgtcacctg gcaggccgac tgcaaagaaa atgcgggcgg
     1501 cgactattac tggactttct tcccacagcc tacgtttgtc agcacgcaga agtattactg
     1561 ccatgttgat aacagttgct atatgaactt cgactttagt gccccggaat accatgaact
     1621 ggcgctgtgg gaagacaaag caacgctgcg ttttgaatgt gctgacacat acatctccct
     1681 gctggaaaaa ttaaccgccc tgctgggacg ccagccagaa ctgcccgact ggatttataa
     1741 cggagtaacg ctcggcattc agggcgggac ggaagtgtgc cagaagaaac tggacaccat
     1801 gcgtaacgcg ggcgtgaagg tcaacggcat ctgggcgcag gactggtccg gtattcgtat
     1861 gacctctttt ggcaaacgcg tgatgtggaa ctggaagtgg aacagcgaaa actacccgca
     1921 actggactca cgtattaagc agtggaacaa agagggcgtg cagttcctgg cctatatcaa
     1981 cccgtatgta gccagcgata aagatctctg cgaagaggcg gcaaagcgcg gttatctggc
     2041 aaaagatgtc gccggtggcg actatctggt ggagtttggc gagttctacg gcggcgttgt
     2101 cgatctcact aatccggaag cctacgcctg gttcaaggac gtgatcaaaa agaacatgat
     2161 tgaactcggc tgcggcggtt ggatggctga cttcggcgag tatctgccca ccgacactta
     2221 cctgcataac ggcgtcagcg ccgaaattat gcataacgcc tggcctgcgc tgtgggcgaa
     2281 atgtaactac gaagcccttg aagaaacggg caagctcggc gagatcctct tctttatgcg
     2341 cgccggttct accggtagcc agaaatactc caccatgatg tgggcgggtg accagaacgt
     2401 ggactggagt ctggacgatg gcctggcgtc ggttgtcccg gcggcgctat ctctggcaat
     2461 gaccggacat ggcctgcatc acagcgacat tggcggttat accaccctgt ttgagatgaa
     2521 gcgcagcaaa gagctgctgc tgcgctggtg cgatttcagc gccttcacgc cgatgatgcg
     2581 cacccacgaa ggtaaccgtc ctggcgacaa ctggcagttt gacggcgacg cggaaaccat
     2641 cgcccatttc gcccgtatga ccaccgtctt caccaccctg aaaccttacc tgaaagaggc
     2701 cgtcgcgctg aatgcgaagt ccggcctgcc ggttatgcgc ccgctgttcc tgcattacga
     2761 agacgacgcg cagacctatt ccctgaaata tcagtacctg ttaggtcgcg acattctggt
     2821 tgctccggta cacgaagaag gtcgcagcga ctggacgctc tatctgccag aggataactg
     2881 ggtccacgcc tggaccggag aaaccttcca cggtggagaa ataactgttg aagcgcccat
     2941 cggcaagccg ccggtctttt atcgcgccga cagcgaatgg gctgcactgt tcgcctcgtt
     3001 aaaaagcatc taatcacgtc cgcccgtggg gaaactcacg ggcaaatgga gaataacgat
     3061 gagtcacatc acaacggaag atccggcaac tttgcgcctg ccctttaaag agaaactctc
     3121 ttacggtatc ggcgatctgg cctctaacat cctgctggat attggtacgc tttatctttt
     3181 gaagttttat accgacgttc tggggctacc tggcacctat ggcggcatta tctttttgat
     3241 ctcgaaattc tttaccgcct ttaccgatat gggaaccggc atcatgttgg attcccggcg
     3301 taagattggc ccgaaaggta agttccgccc tttcattttg tacgcgtcat tcccggtcac
     3361 cttattggca atcgctaact ttatcggcac accgttcgat gtcactggta aaacggtgat
     3421 ggccactatt ctgtttatgc tctacgggct gtttttcagc atgatgaact gctcctatgg
     3481 cgcgatggtg cctgctatta ccaaaaaccc caacgagcgc gcatcgctgg cggcatggcg
     3541 tcagggcggt gctacgctgg gcctgctgct gtgcacggtg ggattcgtgc cggttatgaa
     3601 tcttatcgaa ggtaatcagc aacttggcta tatcttcgcc gccacgctgt tttcactgtt
     3661 cggcctgctg tttatgtgga tctgctactc gggcgtgaaa gagcgttatg tcgaaaccca
     3721 accagccaat ccggcgcaaa agccaggcct gttgcaatct ttccgcgcaa ttgccggtaa
     3781 ccgcccgctg ttcattttgt gcattgccaa cctctgcact ttaggggcgt ttaacgtcaa
     3841 gctcgccatt caggtctatt acacccagta cgtacttaac gatcccatcc tgttgtcata
     3901 tatgggattt ttcagcatgg gctgtatttt catcggcgtg ttcctgatgc ctggcgcagt
     3961 caggcgtttt ggtaagaaga aggtctatat cggcggccta ctgatttggg tgctgggcga
     4021 tctgctcaac tatttcttcg gcggcggctc ggtcagcttc gtggcgttct cctgcctggc
     4081 attcttcggc tcagcgtttg ttaacagcct gaactgggcg ctggtttccg acaccgtcga
     4141 gtacggcgag tggcgtaccg gcgttcgttc ggaaggaacg gtctacaccg gcttcacctt
     4201 ctttcgcaaa gtgtctcagg cgctggctgg tttcttcccc ggctggatgc tgacgcaaat
     4261 cggctatgtg ccgaacgtgg cgcaggctga ccacaccatt gaagggttgc gccagctgat
     4321 cttcatctac ccaagcgcac tggcggtagt caccattgtg gcgatgggtt gcttctacag
     4381 tctgaacgag aagatgtacg tccgcattgt tgaagaaata gaagcccgta aacgcacggc
     4441 gtaataataa acgacgccct gcggggcgtt ataaggagtg attatgtctg accataatcc
     4501 actgacatta aaactgaatc tgtgggaaaa aatcgcctat ggtatgggcg acgttggttc
     4561 gaatttaatg cttagcatcg gtactctgta tctcctcaaa ttttataccg atgaattagg
     4621 gatgccagct tactatggcg ggattatctt tctagtggcg aagtttttta ccgcgtttac
     4681 cgatatgctc accggatttt tactcgattc gcggaaaaat attgggccta aaggcaaatt
     4741 tcgtcccttt attttatatg cagcggttcc ggcggcgtta attgcaacgc tccagtttat
     4801 cgccaccact tttagtttgc cggttaaaac gacgattgcc acggcgctgt ttatgatgtt
     4861 tgggctttca tacagtctga tgaactgctc gtatggcgcg atgatcccgg caattaccaa
     4921 aaacccgaat gagcgcgcgc agctcgctgc ttaccgccag ggtggtgcaa ccatagggct
     4981 attgatttgt accgtggcgt ttattccgtt gcagtcgctt ttttctgact ccaccgtcgg
     5041 ttatgcctgc gcggcactga tgttctccat tggtggcttt atttttatga tgctgtgcta
     5101 cagaggcgtc aaagagcatt atgtggacac aacgccaacc ggacataaag ccagtattct
     5161 caaatcattt tgcgcgatat ttcggaatcc gccattgctg gttttatgca ttgctaactt
     5221 gtgtaccctg gcggcattta atatcaaact ggctattcag gtctattaca cccagtatgt
     5281 gctgaatgat attaatttat tgtcatggat gggattcttc agtatgggat gcatcctcat
     5341 cggcgtatta ctggtgccat taactgtaaa atgttttggt aaaaaacagg tttatttagc
     5401 tggcatggtg ctatgggcag tgggtgatat cctgaattat ttctggggaa gtaactcttt
     5461 cactttcgtc atgttctctt gtatcgcctt ttttggcacg gcctttgtaa acagcctgaa
     5521 ctgggcactg gtgccagata ccgtagatta cggtgaatgg aaaaccggta ttcgtgccga
     5581 aggttctgtt tataccggtt ataccttctt tcgtaaaatt tctgccgcac ttgctggctt
     5641 cttgccaggc attatgctga cgcaaatcgg ttatgttccc aacatcgcgc aaagtgatgc
     5701 gacattacag ggtttgcgtc agctcatttt tatctggcct tgtgcactgg caattattgc
     5761 tgcattgacg atgggattct tttacacact caatgaaaaa cggtttgcgt tgattattga
     5821 ggaaatcaac caacgcaaaa ataaagagat ggcgacagaa gaaaaaacgg cttccgtaac
     5881 gttataaatg tcaatagccg ctatttccat cctggtgggt ggcggcctcc ctacgtttaa
     5941 aaatggactt attatgaaaa atattaatgc aataatttta ttatcttctc tgacctctgc
     6001 ctcagtattc gctggcgcat acgtcgaaaa cagggaggca tataatctcg cctccgatca
     6061 gggcgaggtt atgctacgtg tgggttataa cttcgatatg ggcgcgggta ttatgttaac
     6121 caatacctac acctttcagc gagaagatga actaaaacat ggatataacg aaatcgaagg
     6181 ttggtatccg ttatttaaac caaccgataa attaaccatc cagcccggtg gcttaattaa
     6241 tgataagagt atcggttcgg gtggtgcagt gtatctggac gtcaactata aatttgtacc
     6301 atggtttaat ctgacagtac gaaatcgcta taaccataat aactatagtt caacagattt
     6361 gagcggggaa ctggataata atgacaccta tgaaattggt acctactgga attttaaaat
     6421 caccgataag ttctcctata catttgagcc acattatttc atacgcgtaa atgactttaa
     6481 tagcagcaat ggaaaagatc atcactggga aatcactaac accttccgtt atcgcattaa
     6541 tgaacactgg ctgccttatt tcgaattacg ctggttagat cgtaatgtcg aaccgtacca
     6601 ccgcgagcaa aaccagattc gtatcgggac gaagtatttc ttctgatgtg aaactgccgg
     6661 atgcggcgta aacgccttat ccggcctacg gttgatacgg ttttcgttgg ccggattaag
     6721 cacatccggc cattaattaa gcttcagccg ccagcatctc tttcttacgt tggctaatac
     6781 gcttaaccag cattacggaa accaccgcac cacagaatgc gaagcaggcc ataatgccaa
     6841 acacgatttt gtagccaata atccccggat ttaaatccag aatgtagcca tacagactga
     6901 agcagaacat cgccggggcg taaccaataa agctacccaa cgccatcgcc gcgcctgttt
     6961 tattttcagc aatttttgct tcgccgatag gtgcaaaaaa tacggctcgc tgtgtaaata
     7021 ctatcgcgcc aaagcccagc gtacatgcca tccctaaata gaccggcata ctttcgtgcg
     7081 gcagcataat caacagtacg agcgcagcgg tactgatgat aaaggtgtag catagatatt
     7141 tactcggcga tttcaggatc ttatctgaaa tcatgccacc aatcggtcca ccaatcattt
     7201 tcagacagta ttggttaatg atgccgtaag cccccaccag cgcaacgggc aatagataga
     7261 tgtttttcag gaatggaatg aagaatgtca ggccacagta aaccgcgtaa acgaagaaga
     7321 cgttgaaagc gataagccag atggttttgt ctctcagcac cgaggtcata ctggtgtttt
     7381 gcgatgcccc gtcttctttt ttcacctcaa cggacggtgc ctcttctttg tcattcagga
     7441 caaagaaaat aataatgcct acggcaatca caataaggga atagaaccag atgcctgctt
     7501 taaaacccaa taagccactg ccaaaccagg taaatactgc caacgcagaa aatgccacca
     7561 cggtatcgac aatgccacgt cctgtttcga agaagccaaa caaccgacct tgttgttcgc
     7621 tattgcccaa tcgacttacc gacttgagca agaccggcca gttcatcatg tcgcaagtaa
     7681 cgccaaatag cgcccagaca aagaggatgc cccaataccc cggcattgtc gtcaaataaa
     7741 cacccagtaa tcctgtcgca atgagtgaga atgacatggt gtatctgcgc ggtagtttat
     7801 cggcaaaata aatagacaga aaaaagccca ctgtggtgac aaatgagttt accgacatgg
     7861 cattaccaat ttgaccattg gtcaaatgga aatattcctg catcgggata taaaacgcat
     7921 ctttcagcga cggtaattta taaattgtac cgccacacag tgttaataga ctaaataacg
     7981 cccatttctt tttcgtgagc ataacatccc ctaagcgatt tttaaaatca ctgttttaaa
     8041 ggcaataaga taccgaccat tcatatggaa tggttttatt aactgcgtat gagttaaaac
     8101 ttattaatgg ttcagaagat ggtttgcctc ttgtttgatt tgttgtagta cgttacgcac
     8161 atgatttatc tgattaattg catcttcttt ttctttacgt aaccgcgcat ccagtaaatg
     8221 attttctggt aggggtgttt cactcatctg gcgataaggg atccacggat tatcatcttc
     8281 gtcttcaaag cggaacgccg gcgcatagta atcaacctct tcttccaggc catatgccgt
     8341 cacctgcggc tcatcatcac ccaggcagat aaggtgcgtt aataacgctt tgaacggcat
     8401 atcaccctga cctgaaatac aggctttatg acccaggcca ccctgttctt taacgatcaa
     8461 ggcatcttta atatggacct gggtaatatg cggtgccatc gtttttaagg cgtcgatggg
     8521 atgctcattt gcattgatca tgttcgcaaa atcaaacagt aaggaaagag actccatctc
     8581 actttctttg accagcgaca ccaactcatg actttttaaa tcttcatgct gctcgatcgt
     8641 aaaagtcagg ccgctgtcct gatacgtttc ccgtacatag gcaatgtcgt tagcgataat
     8701 cgataatacg tcgcgcaaat taccttcata acgtggataa aaacgtacgg acgatgcccc
     8761 agttttcaac gcgatggcga cggcttcgtc aatagatgcc ttatctgagg cgctggtttc
     8821 aatatgaata tcaaggttca gacggcgggc tttatcccca aaggcagaga gttctttatc
     8881 gtccatatta ccaagagaaa aacgctcgcc atccagaaca tggattttga cgccacgtag
     8941 attattttca cttgcgatat caagtaaatc agcaggcaac actctttcca gccgcatatt
     9001 taaatgaaag gcataagcat gtaaataaag aggaagagtg tcgacacgtt gtagaatctt
     9061 tcttgcgtta tttattgtca ccataaaaca cctacagatt aatgatgagg tctacggcct
     9121 caaatcgcca atattcaata tctatctgga caatattgcc ttgctggtcg gcgcgatgct
     9181 tttcaattaa tatcgctggc atacccgcag aaccgccaag atacttgctg atatcgcccg
     9241 gcattctgac gggtttgaaa atcaattttt ttactaccgt ctctttttgg tagcactttt
     9301 cccagactgc agaaaatgag tggttttcaa gttgttcaat aaaacccgga gcaacttcag
     9361 gattaatata tgtttcgtga tagaaaactt tatgtccttc cagcgccccc cagccggtga
     9421 tgcgatagag ttcagttgat ggcgtcactg caatcagtgg cgcgagcgtc tcgggaatat
     9481 cgctggtacg gtttttctcg gtaaaccccc aggaaggctc tcgcccttgc tcaattgcgg
     9541 cccgctgaaa gctcgccgac agctccggac tgtaattaaa tcgcggctgg gtcacgaacc
     9601 accccttacg atccttacgg aagattttgg attcggcctc gaggttcaac aacgcctggc
     9661 gcagcgtcat acgtttaatg cccagcagtt ctcccagctc tctttctgaa gggagttttc
     9721 cacccggcgt ggtgatgcca tctttcaacc accgatccag tttctctttt gcattttcta
     9781 cggtggattg attctccgcc attgtttaat ccttttggtt taaaccagat gaagcatctt
     9841 acgcttacgc caaaaaagaa agcaattaac ggtgtatgac tttgcagata attgtgacaa
     9901 agctctctat tgacgtaatc acagacttat aagggagata ctcgcattgg tgtgcgctca
     9961 cgacaggtaa aaaaaaacct gccatcgctg gcaggttttt tatgactaaa aaacgaaatt
    10021 aatcgtcttt cggtgcgcgg ttggcgcgac gacgatcgtt ttccgtcagg tgacgtttac
    10081 gaatacggat agaggtcgga gtcacttcta ccagttcgtc atcatcgatg aactccagag
    10141 cttgttccag agtcatgcgg ataggcggaa ccagaacaac ggcttcgtca gtaccggaag
    10201 cacgcatgtt ggtcagtttc ttaccggtca ggcagtttac agtcaggtcg ttagagcggc
    10261 tgtgaatacc gataatctga ccttcgtaaa cttctgcacc gtgaccgagg aacagcttac
    10321 cgcgatcctg caaaccgaac agcgcgaacg cgaccgcttt accctgaccg ttagagatca
    10381 gtacgccgtt ctgacgctga cccacttcac ccggacgcac gtcgtcgtag tggctgaagg
    10441 tggagtacag cagacccgta ccggaagtca tggtcatgaa ctcagaacgg aagccaatca
    10501 gaccacggct tgggatcaca tagtcgagac gtacgcggcc tttaccgtct ggattcatgt
    10561 ttttcaggtc gcctttacgt tcgcccagcg cctgcatcac agaaccctga tgctgttctt
    10621 caacgtccag cgtcacgttt tcgtaaggct cttgtttacg accgtcgatt tcacggaaga
    10681 ttactttcgg acgagatacc gccagttcga aaccttcacg acgcatgttt tcgataagaa
    10741 cagacaggtg cagttcgcca cgaccagaaa cgcggaacgc atcggcgtct tcggtttctt
    10801 ctacgcgcag cgcaacgttg tgtaccagtt ctttgttcag acgatccagg atctgacgag
    10861 acgttacgaa cttaccttct ttaccgcaga acggcgaggt gttaacgcag aagaacatag
    10921 aaacggtcgg ctcatcaacg gagagtgccg gcagcgcttc aacgttttgc gtgtcgcaaa
    10981 cggtgtcaga aatgttcagt tcgccaagac ccgtgatcgc cacgatatcg ccagcttccg
    11041 ccagatcggt ttcgatacgt tccagaccga ggtggcccag cactttaccg actttcgcgt
    11101 tgcgggtttt gccttcgcta tcgatgatag taacctgctg gttcggcttc actttaccgc
    11161 gcttgatgcg gccaatgccg ataacgccaa catagctgtt gtaatcgagc tgagaaatct
    11221 gcatctggaa cggaccgtca aggtcaacgt ccggcgcagg aacgtggtca acaatcgcct
    11281 ggtacagcgg ggtcatgtct tccgccatat cttcgtggtc cagacccgcg ataccgttca
    11341 gcgcagaagc gtaaacgatc gggaagtcca gctgctcgtc ggtcgcgtcg aggttaacga
    11401 acagatcgaa tacctgatcc acaacccaat caggacgcgc gccagggcgg tcaactttgt
    11461 tgataacaac aatcggcttc aggccgtaag caaacgcttt tttggttacg aagcgcgttt
    11521 gcggcatcgg gccgtcaaat gcgtcaacca ccagcagcac tgagtctacc atggacatta
    11581 cacgttcaac ttcaccaccg aagtcggcgt gccccggggt atcaacgatg ttaatacggt
    11641 aatcattcca tttgatagcg gtgtttttcg cgaggatggt aatcccacgc tctttctcca
    11701 aatcgttgga gtccatcacg cgctcttggg tttcggcacg agagtcgaac gtaccggatt
    11761 gttggagcag cttgtctacc agggtggttt taccatggtc tacgtgcgcg atgatggcga
    11821 tattacgcaa tttttcgatc acaactttgc ctcaggcatt agaaatagcg cgttattgta
    11881 cacggattaa tcgcactaca aaacaggatc acaaacatcc tccgcaaaca agtattgcag
    11941 agtccctttg tgatcgcttt cacggagcat aaaaagggtt atccaaaggt cattgcacca
    12001 atatggtgct taatgtttcc attgaagcac tatattggtg caacattcac atcatggtgc
    12061 agcccttttg cacgatgtta agcatgataa cgccttttag ggacaattta aaagttggca
    12121 cagttttcgc tttatctttt ttacggcgac acggccaaaa taattgcaga tttcgttacc
    12181 acgacgacaa tgaccaatcc aggagagtta aagtatgtcc gctgaacacg tactgacgat
    12241 gctgaacgag cacgaagtga agtttgttga tttgcgcttc accgatacca aaggtaaaga
    12301 acagcacgtc actatccctg ctcatcaggt gaatgctgaa ttcttcgaag aaggcaaaat
    12361 gtttgacggc tcctcgattg gcggctggaa aggcattaac gaatccgaca tggtgctgat
    12421 gccagacgca tccaccgcag tgattgaccc gttcttcgcc gattctaccc tgattatccg
    12481 ttgcgacatc cttgaaccag gcaccctgca aggctatgac cgtgacccgc gctccatcgc
    12541 gaagcgcgct gaagattacc tgcgttccac tggcattgcc gacaccgtac tgttcggacc
    12601 agaaccagaa tttttcctgt tcgatgacat ccgtttcgga tcatctatct ccggttccca
    12661 cgttgctatc gacgatatcg aaggcgcatg gaactcctcc acccaatatg aaggtggtaa
    12721 caaaggtcac cgtccagcag tgaaaggcgg ttacttcccg gttccgccgg tagactcggc
    12781 tcaggatatt cgttctgaaa tgtgtctggt gatggaacag atgggtctgg tggttgaagc
    12841 ccatcaccac gaagtagcga ctgctggtca gaacgaagtg gctacccgct tcaataccat
    12901 gaccaaaaaa gctgacgaaa ttcagatcta caaatatgtt gtgcacaacg tagcgcaccg
    12961 cttcggtaaa accgcgacct ttatgccaaa accgatgttc ggtgataacg gctccggtat
    13021 gcactgccac atgtctctgt ctaaaaacgg cgttaacctg ttcgcgggcg acaaatacgc
    13081 tggtctgtct gagcaggcgc tgtactacat tggcggcgta atcaaacacg cgaaagcgat
    13141 taacgccctg gcaaacccga ccaccaactc ttataagcgt ctggtcccgg gctacgaagc
    13201 accggtaatg ctggcttact ctgcacgtaa ccgttctgct tctatccgta ttccagtggt
    13261 ttcttctccg aaagcgcgtc gtatcgaagt acgtttcccg gacccggcgg ctaacccgta
    13321 cctgtgcttt gctgccctgc tgatggccgg tcttgatggt atcaagaaca agatccatcc
    13381 gggcgaagct atggacaaaa acctgtatga cctgccgccg gaagaggcga aagagatccc
    13441 acaggttgca ggctctctgg aagaagcact gaacgaactg gatctggacc gtgagttcct
    13501 gaaagccggt ggcgtgttca ctgatgaagc gattgatgcg tacatcgctc tgcgtcgcga
    13561 agaagatgac cgcgtgcgta tgactccgca tccggtagag tttgagctgt actacagcgt
    13621 ctaatagttg aagttgtact acccggcgca acaacgccgg gatttagttg ccgtggaaac
    13681 tttcagccca tctctgcatg ggcttttttc tccgtcaatt ctctgatgct tcgcgctttt
    13741 tatccgtaaa aagctataat gcactaaaat ggtgcaacct gttcaggaga ctgctttatg
    13801 gcaacaggca cgcagcccga tgctgggcag atcctcaact cgctgattaa cagtattttg
    13861 ttaatcgatg acaacctggc gatccattac gccaaccccg ccgcgcaaca actgctcgcc
    13921 caaagctccc gcaaattgtt tggtacgccg ttaccggaac tgttgagcta cttctcatta
    13981 aatatcgagc tgatgcaaga aagtctggag gcggggcaag gttttaccga taacgaagtg
    14041 acgctggtca tcgacgggcg ctcgcatatc ctttctgtga cggcccagcg tatgccggac
    14101 ggcatgatcc tgctggagat ggcgccgatg gataaccagc gccgcttaag ccaggaacag
    14161 ctacaacatg ctcaacaagt tgctgcccgt gatttagtgc gtggcctggc acatgagatt
    14221 aaaaatccgc ttggcggttt acgtggcgcg gcgcagctac tcagcaaagc attacctgac
    14281 ccgtcactac tggaatatac caaagtgatt atcgaacagg cggaccggct gcgaaatctg
    14341 gtcgaccgtc tgttggggcc gcagctgccc ggtacgcgtg ttaccgaaag tattcacaaa
    14401 gtagctgaac gcgtggtaac gctggtgtcg atggaactgc cggacaacgt gcggttgatt
    14461 cgtgattacg atcccagcct gccggaactg gcgcacgacc cggatcaaat tgaacaggtc
    14521 ttgctgaata ttgtgcgcaa tgcgctacag gcgctggggc cggaaggcgg tgaaatcatt
    14581 ctgcgtaccc gcaccgcgtt tcaactgacc ttacacggcg aacgctatcg gctggcggcg
    14641 cggattgatg tggaagataa cgggccaggc attccgcctc atttgcagga tacgctgttt
    14701 tacccgatgg tcagcggccg cgaaggtggc accgggcttg gcttatccat cgctcgtaat
    14761 ttgattgatc agcattcagg caaaattgaa tttaccagtt ggccagggca taccgagttc
    14821 tcggtttacc tgcctatcag gaaataaagg tgacgtttat gcaacgaggg atagtctggg
    14881 tagtcgatga cgatagttcc atccgttggg tgcttgaacg tgcgctcgct ggagcgggtt
    14941 taacctgtac gacatttgag aacggcgcgg aggtactgga ggcgctggcg agcaaaacgc
    15001 cggatgtgct gctttcagat atccgtatgc cgggaatgga cgggctggcg ctgctcaagc
    15061 agattaaaca gcgccatccg atgcttccgg tcatcattat gaccgcacat tccgatctgg
    15121 atgctgccgt cagcgcctat caacaagggg cgttcgatta tctgcccaaa ccgtttgata
    15181 tcgacgaagc cgtggcgctg gttgagcgcg ccatcagtca ttaccaggaa cagcagcagc
    15241 cgcgtaatgt tcagcttaac ggcccaacga ccgatatcat cggcgaagcg ccagccatgc
    15301 aggacgtgtt ccgtattatc ggtcgacttt cgcgttcttc tattagcgtg ctgattaacg
    15361 gcgaatccgg taccggtaaa gaactggtcg ctcatgccct gcatcgccac agtccgcgag
    15421 ccaaagcgcc gtttatcgcg ctgaatatgg cagctatccc aaaagatttg atcgaatccg
    15481 aactgtttgg tcacgagaaa ggcgcgttta ccggcgcgaa taccattcgt caggggcgtt
    15541 ttgaacaggc tgatggcggt acattatttc tcgatgaaat tggcgatatg ccgctggatg
    15601 tgcagacgcg tttgctgcgc gtgctggcag acggtcagtt ttaccgcgtt ggcggctatg
    15661 cgccggtgaa agtggatgtg cggattatcg ctgccactca ccagaatctc gaacagcggg
    15721 tgcaggaagg taagttccgt gaggatctgt tccaccgcct gaacgttatc cgcgttcatc
    15781 tgccgccact gcgtgagcgt cgggaagata ttccccgcct ggcacgccat tttttacagg
    15841 ttgctgcgcg cgaactgggc gtagaagcga agttgctgca tccggaaacc gaagctgctc
    15901 tgacgcgtct ggcgtggcca ggcaacgtgc gccagctgga aaacacctgc cgctggctaa
    15961 cggtgatggc cgccgggcag gaagtgttga ttcaggattt gcccggcgaa ctgtttgaat
    16021 caacggttgc ggagagtact tcgcaaatgc aaccggacag ttgggcgacg cttcttgcgc
    16081 agtgggcaga cagagcgctg cgttccggtc atcaaaatct gctttccgaa gcgcagccag
    16141 agctggagcg gacgttactg acgaccgcgt tgcgacatac gcaggggcat aaacaggaag
    16201 cggcgcggct actcggctgg ggccgcaaca ctctgacgcg taagttaaaa gagctgggga
    16261 tggagtgatt cacggcttgt gtgtaaagat tgattattga gcgcaaattg ctggtatttt
    16321 gcgctttact gttccgataa gttcagtatg atcttgcccg gaaaacgggg agagtcatta
    16381 tgctggaatc aataattaat ctggtatcga gtggcgcagt tgacagccac acaccgcaaa
    16441 ctgccgttgc tgccgtgctg tgtgccgcga tgattgggct gtttagttga gtttcagatt
    16501 gctgatgaag tgctctttgt tcatgccgga tgcggcgtga acgccttatc cggtctacaa
    16561 aagcaatatt ttcaacaggt tttgtaggcc tgataagcgt agcgcatcag gcaatcttgt
    16621 tctcagcaca ttgatggcgc tttcgttttc tactttgtaa acgaagcgcc attcactatt
    16681 taaatcaccc gagagaactg ctgcatccgc gctttctggc gcagataggt atcaaagcac
    16741 atgcaaatgt tgcggatcag caagcgacct ttcgccgtca cctgaatccc cttctcatcc
    16801 acatccacca gcccatcttt tgctaacggg gcgagcagct tgagatcttc cgcaaagtaa
    16861 tcagcgaagt gcaaatccca ctgtttctca ataggggcgt aatccagacg gaagttgcag
    16921 atgagcgact taatcacatc gcggcgaata cagtcatcac gcgttagcgc aataccacgc
    16981 cacagcgcat tgccttgttc atccacttgc tgatagtact gcttcaactc tttctggttc
    17041 tgcgcgtagc agtcgccaat catgctgatg gcggaaacgc ccatccccag cagatcggta
    17101 tcgccctgag tggtgtagcc ctggaaatta cgatgcagca cgccttcacg ctgggccacc
    17161 gccagctcgt catccggacg ggcaaagtga tccataccga taaactgata gcccgattgc
    17221 gtcaggaagg cgatggtttc ctgcaggata tcgagttttt gctgcggact cggcaggtca
    17281 gcatctttga ttttgcgctg agcagcaaaa atggtcggca gatgcgcata gttaaagacg
    17341 ctcagacgat cagggttcag ctccgccacg cgtttcaggg taaaggcgaa actttccggc
    17401 gtctgtttcg gcaggccgta aatcaggtcg atgttggtgg aggtaaagcc aatctcacgc
    17461 gcatggttaa gcagcgcaaa gatgaactct tcatcctgct cgcggttaac cagacgttgc
    17521 acttctttgt tgaagtcctg cacgcccata ctcaggcgat taaagccctc ggcgcgtaaa
    17581 tgatcgagta catccagttc gatttcccgc ggatcgactt cgatcgaaat ctccgcatcg
    17641 gcattgaact ggaagttttc gcgcagcagc ttcatcaggc ggctgatttg cgctttatta
    17701 agatacgtcg gcgttccgcc gccccagtgc agctggctga cgtgacgccc ggcgaacagc
    17761 ggtgcacgat ggacgatttc ttgctccagc gcgtccagat actgatcggc cttgtgctgc
    17821 tggcgagtaa caatcttatt gcaaccgcag aagtaacaaa gcttatggca gaacgggata
    17881 tgtacgtaga gagataatgg acgctcagga tagcgcgcca cggcttgtaa aaacgcctgt
    17941 tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg aggtgtatcg tggcccggaa
    18001 tagttatatt tctggatcag ggccagatcc cagtcgattt gctgtacaga catgctcact
    18061 ccttccgatg gcgtctctgg cggctacggc gtaccggcgg atgcccgttt cgggtcatca
    18121 gccggttgcg cagccacttt tgtcgccgcg acaaccgctg tagtttaacg aataaccaca
    18181 ccagataaca tataaccgga agggttatca gcagaacggg aaggcccacg gcggcaatcc
    18241 gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt ctcgtcttct tcctcttctt
    18301 cgtcatcgtc ataagagagg ccgagtttct gcatcaactc atcaatacga tccagtttag
    18361 catccaccca ggattgctct tcggcactca gggtttcgcc tgcttccaga cgttccagca
    18421 gcgcatccag acgctcatcc gtttccagta attccaactc cgcctgcggt gaaagcatag
    18481 gtttctcact cttcggtttg tgctgtttgg tgactttttc agtcacgccc aatggaatag
    18541 gggttttact gccaatacgt ggatcttttg gtgcgttctg gcctttgctg cctgacgtgg
    18601 tgttaccgcc cgctgcgcgg ctgcccggcg catgaccacg acgttttttc tgacgcttgc
    18661 ggtcacgagc ttcctgattc agctcttcgc gcgttttgcg acgcgctttt gcatgacctt
    18721 tgctgcgtga tggtgaagat ggtttcataa tggttcgtct tttagccgtt ttattcagta
    18781 tagatttgcg gcggaatcta acagaaagca agcaaagaaa aaaggcgaca gattactctg
    18841 tcgccttttt tcctgactca taacccttaa cgggtcttac aatccttgca ggccgccaac
    18901 acaccctgtt tttccattag ccggaacaat ccgtgttgat tccatttccg tttaattacg
    18961 tctccggacg tttgtcttcc tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct
    19021 catcctgagt cattgttcct gttagcgtcc tcgctctatg ggtgctactt tacgcctttg
    19081 ctttaaacaa acaatcaacc gccatcagta tttgcacatt ttcagagttt tatataaaag
    19141 caattaactg atttatatag cttatttgac atgcatcgca gaattttctc tgctatttcg
    19201 cataaagact atcggcaatg tctcacaaag cgtatggctt ttacttacaa cgctcacgcc
    19261 gaaaaatccg ccttttatta tggttcaggt ataatcgccg caaagctttg actgacggag
    19321 acgaccgctt tgactaattt gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat
    19381 attcgccacc taccttccga taccggaatt gaagtggctt ttgcaggccg ttccaacgca
    19441 ggtaaatcca gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg tacctcaaaa
    19501 accccaggac gcacccagct tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt
    19561 gacttgcctg ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgc
    19621 gcgctcggcg agtacctcga aaaacgtcag agcctgcaag gtctggtggt gctaatggat
    19681 attcgccatc cgctgaaaga tttggatcag cagatgattg agtgggcggt agacagcaat
    19741 atcgccgtgc tggtgttgct gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg
    19801 caattgaata tggtgcgtga agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg
    19861 ttttcttcgt tgaagaaaca aggcgtggac aagctgcggc agaaactgga tacctggttt
    19921 agcgagatgc agcctgtaga agaaacgcag gacggcgaat aattttcttg ccttaatgct
    19981 tgtgccggat gtgacgtatc cggcccataa attcaataga ttacaattct accgttggcc
    20041 tgataaacat ggcgcatcag gcattacgga tcttttcttt cgcccaataa aaaacgcccc
    20101 agtcattact gactggggcg gctaaaatat tcagccaaat ccgattacgt gaagtaaaag
    20161 gtctgaaaga tagaacatct tacctctgta ccctacgcga ctaactttac tcttttttgt
    20221 tcagttcaga aagcactttt tgtaattttt tttcagttgt tgcataggaa tttctaatag
    20281 ccatcacaaa acgtgacagc ttatgttgct tacttacgaa aaaagggcat gttcaggcga
    20341 atcttagtgc gcctgatccc agttttcgcc actccccact tccaccagca acggcacatc
    20401 cagacgggta cagttttcca tcagttgatg aatctgcttc gcgacggcat cgacatcatc
    20461 tttatggact tcaaatacca gttcatcgtg tacctgcatg atcatacgta cacgcggttg
    20521 ctcagcctgt aaccacgcat caacggcaat catcgcccgt ttgataatgt cggcggcggt
    20581 tccctgcatt ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag caccattgct
    20641 ggatttgata tccggcagat acagacggcg tccgtccagc gtttcaacgt agccctgctc
    20701 tttcgcctga gcacgggtgc gttccatata ctccagcacg ccagggtagc gttcgaagta
    20761 aaggtccatg tacttctgcg cttctttacg tggaatgttc aattgccgcg ccagaccgaa
    20821 agcactcatg ccataaatca gaccaaagtt gatcgctttc gcgctacggc gttgctcgct
    20881 ggtgacggtt tccagtggca aaccaaacac ttctgccgcc gttgcccggt ggatatcttt
    20941 tccttccgcg aatgcggtca gcaagccttt gtcacgcgag agatgcgcca taatgcgcag
    21001 ttcaatctgc gagtagtccg ctgagacaat cacataatcc tctggcgcaa taaacgcctg
    21061 gcggatacga cgaccttctt cgttacgcac cggaatgttt tgcaggttag gatcggttga
    21121 cgataaacgt cccgttgcag ttactgcctg gtgataagag gtatgcacac gcccggtttt
    21181 cgggttgatc atcagcggca gcttgtcggt gtaagtcgac ttcagcttcg ccagaccacg
    21241 atactccaga atcacttttg gcaacggata gtccagcgcc agttcttcca gtacctcttc
    21301 cgacgttgac ggcgcgccac ccggcgtttt cttcagcggt ttaatgccct gtttttcaaa
    21361 cagaatggtt tgtaactgct tggtggaaga aaggttaaat tcctcacctg caatttcatg
    21421 cgctttcttt tccagctcag ccagacgaag ggtgagctct tcagaatgat tgtgcagcac
    21481 tttcggatcg atcttcacac cgttacgttc aatgcgtgaa agcaccggca ccagcggcat
    21541 ttcgatattc tcgaagacgt tcaacggccc tttgtgtttt tgcagatccg gccacatttt
    21601 cagatgcaac tgcaaggtga catctgcatc ttcggcggcg taacgtccgg cctcttcgag
    21661 ggcaatctgg ttaaaggtca gttgattttt gcctttaccc gcaatctctt caaaagtgat
    21721 ggttttgtgc ttcaaccaac gttccgcgag gctgtccata tcgtgacgcc cggcaacgct
    21781 attgagaatg taggactcca gcatggtatc aaacgcaatc ccacgcagct caatgccgta
    21841 attcgccaga ataccgcgat cgtatttcag gttttgcccg accttcagcg ccttttcatc
    21901 ttccagcagc ggttttagca actcgagtgc acgctcgcga gagatttgat cgggcgcatc
    21961 aagataatca tgagcaaccg gaatatatgc cgctacgcct ggctcgatag caaaagaaag
    22021 cccgaccagg ttagcagaga tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac
    22081 cggcgctttt tccagcttcg caatccacgc tttcagtgtt tcttcatcaa ggatggtgac
    22141 gtagttgtca taagaaatca ccgttgccgt cacttctggt gcttcgtctg caacactggt
    22201 ttcctgtggc ttcgcggctg gttttgcccc tttggcctgt aaccatttgc ccgcttcgac
    22261 atcagcagtc cagcgtttga actcatactt tttgaacagc cccaacaact cttccgctgc
    22321 cggttgctgc acttccagtt gttcacaggt cagctccagt tcaacgtcgg ttttaatcgt
    22381 cgccagctgg tatgagagat aagcaacttc tttgttttgc tcgagcttcg ctgccattgt
    22441 tttcgcgcca cggaagctca acccagcaat tttttctggc tcggcataca gcgtatccag
    22501 cccgccaaga ccttgcagca atgcctgcgc ggttttttct ccgacgcccg gtacgccagg
    22561 aatgttatca gaggagtcac ccatcagcgc caggaaatcg atgatcagtt ctggcggcac
    22621 gccgtactta ttcaccacct cttccggtcc gaggatggta ttcgtcatgg tattgataag
    22681 cgtaatattt ggcgtcacca gctgcgccat atctttatcg ccagtgctga tcagcaccgg
    22741 acgcccggct ttttcggctt cgcgcgccag agtaccgata acgtcgtccg cttctacgcc
    22801 agaaaccgcc agcagcggca gtcccatcgc tttaaccatc gcgtgcaagg gttcgatttg
    22861 tgctcgcaga tcgtccggca ttggcgggcg atgtgatttg taatgttcaa acagttcatc
    22921 acgaaaggtt tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt tatattgcat
    22981 gatcagactg cgcagcatgt tgaggacacc atacatcgca ccggtcggct cgcctgcgct
    23041 gttagtcagc gggggaaacg cgtgatatgc gcgataaaga taagatgaac catctacaag
    23101 gataagtgga ttttggggga tctgaaccat aatgtccgtg cctgtttatc agattatggg
    23161 taaaggatgc cacagacagg ataaaaacat gagtttttca ccgcttttcg tgaaaatctt
    23221 ttgtagatct tctggatcgc tcgcaaatcg tcatgtggat aactttgtgc ataaattatt
    23281 tac
//
LOCUS       DAZUCE010000063        23172 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZUCE010000063 DAZUCE010000000
VERSION     DAZUCE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.063"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            219..1358
                     /gene="wza"
                     /locus_tag="ACHY03_002042"
     CDS             219..1358
                     /gene="wza"
                     /locus_tag="ACHY03_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFS3905274.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1364..1807
                     /gene="wzb"
                     /locus_tag="ACHY03_002043"
     CDS             1364..1807
                     /gene="wzb"
                     /locus_tag="ACHY03_002043"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFS3905275.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            1810..3972
                     /gene="wzc"
                     /locus_tag="ACHY03_002044"
     CDS             1810..3972
                     /gene="wzc"
                     /locus_tag="ACHY03_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFS3905276.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEEREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4065..4904
                     /gene="wcaA"
                     /locus_tag="ACHY03_002045"
     CDS             4065..4904
                     /gene="wcaA"
                     /locus_tag="ACHY03_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFS3905277.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVSALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            4907..5395
                     /gene="wcaB"
                     /locus_tag="ACHY03_002046"
     CDS             4907..5395
                     /gene="wcaB"
                     /locus_tag="ACHY03_002046"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFS3905278.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5392..6609
                     /gene="wcaC"
                     /locus_tag="ACHY03_002047"
     CDS             5392..6609
                     /gene="wcaC"
                     /locus_tag="ACHY03_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFS3905279.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKYTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6584..7804
                     /gene="wcaD"
                     /locus_tag="ACHY03_002048"
     CDS             6584..7804
                     /gene="wcaD"
                     /locus_tag="ACHY03_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFS3905280.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNIHPK"
     gene            7930..8676
                     /gene="wcaE"
                     /locus_tag="ACHY03_002049"
     CDS             7930..8676
                     /gene="wcaE"
                     /locus_tag="ACHY03_002049"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFS3905281.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAAYF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8692..9240
                     /gene="wcaF"
                     /locus_tag="ACHY03_002050"
     CDS             8692..9240
                     /gene="wcaF"
                     /locus_tag="ACHY03_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFS3905282.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATLFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9267..10388
                     /gene="gmd"
                     /locus_tag="ACHY03_002051"
     CDS             9267..10388
                     /gene="gmd"
                     /locus_tag="ACHY03_002051"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFS3905283.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10391..11356
                     /gene="fcl"
                     /locus_tag="ACHY03_002052"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10391..11356
                     /gene="fcl"
                     /locus_tag="ACHY03_002052"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFS3905284.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11359..11838
                     /gene="gmm"
                     /locus_tag="ACHY03_002053"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11359..11838
                     /gene="gmm"
                     /locus_tag="ACHY03_002053"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFS3905285.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLCLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYCWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11835..13058
                     /gene="wcaI"
                     /locus_tag="ACHY03_002054"
     CDS             11835..13058
                     /gene="wcaI"
                     /locus_tag="ACHY03_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFS3905286.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQTLLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13061..14497
                     /gene="cpsB"
                     /locus_tag="ACHY03_002055"
     CDS             13061..14497
                     /gene="cpsB"
                     /locus_tag="ACHY03_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFS3905287.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLSCPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14690..16060
                     /gene="cpsG"
                     /locus_tag="ACHY03_002056"
                     /gene_synonym="manB"
     CDS             14690..16060
                     /gene="cpsG"
                     /locus_tag="ACHY03_002056"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFS3905288.1"
                     /translation="MKKLNCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16115..17509
                     /gene="wcaJ"
                     /locus_tag="ACHY03_002057"
     CDS             16115..17509
                     /gene="wcaJ"
                     /locus_tag="ACHY03_002057"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFS3905289.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17511..18989
                     /gene="wzxC"
                     /locus_tag="ACHY03_002058"
     CDS             17511..18989
                     /gene="wzxC"
                     /locus_tag="ACHY03_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFS3905290.1"
                     /translation="MNLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19265..20545
                     /gene="wcaK"
                     /locus_tag="ACHY03_002059"
     CDS             19265..20545
                     /gene="wcaK"
                     /locus_tag="ACHY03_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFS3905291.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQVMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20542..21762
                     /gene="wcaL"
                     /locus_tag="ACHY03_002060"
     CDS             20542..21762
                     /gene="wcaL"
                     /locus_tag="ACHY03_002060"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFS3905292.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21773..23167
                     /gene="wcaM"
                     /locus_tag="ACHY03_002061"
     CDS             21773..23167
                     /gene="wcaM"
                     /locus_tag="ACHY03_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFS3905293.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEVQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKDLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAASFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
BASE COUNT         5607 a         5860 c         6359 g         5346 t
ORIGIN      
        1 tcagtgtatt ggtagctaaa aagccagggg cggtagcgtg tctggatgcc tgaaagacca
       61 gtctgaatta ttctgtcaat agcctgcgga tgaaatcaat ttttttagga ctgatgccag
      121 ttaaattttt attcaattaa ctgcaaggta aatgtgcatg gcgacattgc tattaatagt
      181 gcacaggata attactctgc caaagtgata aataaacaat gatgaaatcc aaaatgaaat
      241 tgatgccatt attggtgtca gtaaccttga taagcggttg cacagtactt ccgggcagca
      301 atatgtcgac gatgggcaaa gacgtcatca aacagcagga cgctgatttc gatctcgaca
      361 aaatggtgaa tgtttatccg ctgaccccgc gcctgattga ccaattacgc ccacgcccga
      421 atgtagcgcg ccccaatatg acgctggaaa gcgagatcgc gaattaccag tatcgcgtcg
      481 ggccggggga cgttcttaat gtcaccgtct gggatcaccc ggaactcacc acgccagccg
      541 gtcagtaccg cagctccagc gacaccggca actgggtaca gcctgacggc actatgtttt
      601 acccgtatat cggcaaggtt cacgtagtcg ggaaaacgct cgctgaaatc cgcagtgata
      661 ttaccgggcg cttagcgacg tacatcgctg acccgcaggt ggacgttaat atcgccgcct
      721 tccgctcgca aaaagcctat atctccggcc aggtgaataa atccggtcaa caggcgatta
      781 ccaacgtacc actgaccatt ctcgatgcca tcaacgccgc aggtggcctg accgacaccg
      841 ctgactggcg caacgtggtg ctaacacaca atggtcgtga agagcgcatt tctttgcagg
      901 cgctgatgca aaacggcgac ctcaaccaga atcgactgct ttaccccggc gatattctct
      961 acgtgccgcg taatgatgat ctgaaagtat ttgtgatggg tgaagtgaag aaacagagca
     1021 ccctgaaaat ggactttagc ggcatgaccc tgaccgaagc cctgggcaat gctgaaggca
     1081 tcgacatgac cacctccaac gccagcggca tctttgtcat tcgtccgctg aaaggcgagg
     1141 gcgggcgtaa cggcaagatt gccaatatct accagctgga tatgtccgat gccacgtcgc
     1201 tggtgatggc gacagaattc cgcctgcaac cttatgacgt ggtgtatgtc accaccgccc
     1261 cggtttcccg ctggaaccgt ctgatcaatc agttgctgcc aactattagc ggtgtccgtt
     1321 acatgacgga tacagccagc gacattcata actggtaatc gtcatgttta acaacatctt
     1381 agttgtctgt gtcggcaata tttgccgttc cccgacggcg gaacgcttac tgcaacgtta
     1441 tcacccggag ctgaaagtgg agtccgctgg actcggcgcg ctggtcggta agggcgctga
     1501 tcctaccgct atcagcgtcg ccgcagaaca tcaactgtct ctggaaggcc actgtgcacg
     1561 acaaatcagc cgccgcctgt gtcgcaacta tgacctgatt ttgaccatgg aaaagcgcca
     1621 tatcgaacgc ttatgcgaga tggcaccgga gatgcgcggc aaagtgatgc tgtttggtca
     1681 ctgggataac gaatgtgaaa tccccgatcc gtatcgcaaa agccgggaaa cgtttgcagc
     1741 ggtgtacaca ttacttgaac ggtctgcccg ccagtgggcg caggcattga acgcagagca
     1801 ggtataagaa tgacagaaaa agtaaaacaa catgccgctc cggtaacggg cagtgatgaa
     1861 atcgatattg gtcgcctggt cggcaccgtc attgaagcgc gctggtgggt gattggcatc
     1921 accgctgtat tcgccctttg tgccgtggtt tacaccttct tcgccacgcc gatttatagt
     1981 gccgacgcac tggtacaaat cgagcaaagc agcggcaatt cgttagtgca ggatatcgga
     2041 tcggcgttag ccaacaaacc gcctgcatcg gacgccgaga tccagttgat tcgttcgcgc
     2101 ctggtgcttg gtaaaacggt ggacgatctc gacctcgata ttgcggtgag caaaaacacg
     2161 ttcccgattt tcggtgcggg ctgggatcgc ctgatgggac gtcagaacga aacggtgaaa
     2221 gtgaccacct ttaaccgccc gaaagagatg gcggatcagg tgtttacgct taatgtgctg
     2281 gacgataaaa actacaccct gagcagtgat ggcggcttta gcgcccgtgg gcaagcgggc
     2341 cagatgctga aaaaagaagg cgtcacgctg atggttgaag ccattcacgc ccgcccgggc
     2401 agtgagttta ccgtcaccaa atactccacg ttggggatga tcaaccaact gcaaaacagc
     2461 ctgacggtaa cggagaacgg caaagacgca ggcgtactga gcctgactta taccggtgaa
     2521 gagcgcgaac agatccgcga cattcttaac agcatcgccc gtaactatca ggaacaaaat
     2581 attgagcgca aatcggcgga agcgtcgaaa agtctcgctt tcctcgcgca acagttaccg
     2641 gaagtacgta gccgccttga tgttgccgaa aacaaactga atgccttccg tcaggataaa
     2701 gattctgttg atctgccgct ggaagcgaaa gcggtgctcg attcgatggt gaacatcgac
     2761 gcgcagttga acgaactgac ctttaaagag gcggaaatct ccaagctgta caccaaagtt
     2821 cacccggcgt accgcacgct gctggagaaa cggcaggcgc tggaagacga aaaagccaaa
     2881 cttaatggtc gcgtaacggc gatgccgaaa acccagcagg aaattgtgcg tctgacccgc
     2941 gatgtcgagt ctggtcagca ggtctatatg caactgctga ataaagagca ggagctgaaa
     3001 atcaccgagg ccagcaccgt cggcgatgtg cgcattgttg acccggcaat cacccagcca
     3061 ggtgtactga aaccgaagaa agggctgatt atccttgggg cgattatcct cggcctgatg
     3121 ctttctatcg tgggtgtact gctgcgctcg ttgtttaatc gcggcattga aagcccgcaa
     3181 gtgctggaag aacacggtat cagcgtctat gccagcatcc cgttgtcgga atggcagaaa
     3241 gcgcgcgata gcgtcaaaac catcaaagga gttaaacgct ataaacagag ccagctactg
     3301 gcggtgggga atccaaccga tctggcgatt gaagccatcc gcagtctgcg taccagtctg
     3361 cacttcgcga tgatgcaggc gcagaacaat gtgttgatga tgaccggggt tagcccgtca
     3421 atcggtaaaa cctttgtctg cgccaacctg gcggcggtga tcagccagac caataaacgg
     3481 gtgttgttga tcgactgcga tatgcgcaaa ggctacaccc acgagctgtt gggcaccaat
     3541 aacgtcaatg gcctgtcgga aattctgatt ggtcagggcg atattactac cgcagctaaa
     3601 ccgacctcta ttgccaaatt tgacctgatc ccgcgcggtc aggtgccgcc aaatccttct
     3661 gaactgttaa tgagcgaacg ctttgccgaa ctggtgaact gggcgagtaa aaactatgac
     3721 ctggtgttga ttgatacgcc gccgattctg gcagtgaccg atgcggcaat tgttggtcgt
     3781 catgtcggaa ccacgttaat ggtggcgcgc tatgcggtca acacattgaa agaagtggaa
     3841 accagtctga gccgctttga gcaaaacggt attccggtga aaggggtgat tctgaactcc
     3901 atcttccgcc gcgccagcgc gtatcaggat tatggctatt acgaatacga atataagtcg
     3961 gatgcgaaat aacgacgcct gcattcgcac cgccccgtag gccggataag gcgctcacgc
     4021 cgcatccggc aagcaaacca gctcataagc cgggagtaca acctatgaaa gacaatccgc
     4081 tgatctcaat ctatatgcca acctggaacc gccaacaact ggcgattcgg gcgataaaat
     4141 cggtcctgcg ccaggactac agcaactggg agatgatcat cgtggatgat tgctccacct
     4201 cctgggaaca actgcaacag tacgtcagcg ccctcaacga tccgcgtatc acctacattc
     4261 acaacgacat taacagcggg gcgtgcgcgg tacgtaacca ggctattatg ctggcgcaag
     4321 gggaatacat taccgggatc gatgacgatg acgaatggac acccaaccgt ctgagcgtct
     4381 tcctcgccca taaacagcaa ctggtcactc acgccttttt gtacgctaac gattacgttt
     4441 gccagggcga agtctattcc caaccggcaa gcttaccgct gtatcccaaa tcgccgtatt
     4501 cacgccgcct gttctataaa cgcaatatca tcggcaatca ggtctttacc tgggcgtggc
     4561 gtttcaaaga gtgtttgttc gataccgaac tgaaagcggc acaggattac gacatcttcc
     4621 tgcggatggt ggtggagtac ggcgaaccgt ggaaagtgga agaggcgacg cagatcctgc
     4681 atatcaatca cggtgaaatg cagatcacct cgtcaccgaa aaaattctcc gggtacttcc
     4741 acttttaccg caagcacaaa gacaaatttg accgcgccag caaaaaatat cagctgttta
     4801 ctctctacca gatccgcaat aaacgcatga cctggcgcac tttgctgacg ctgctgtcgg
     4861 tgcgtaacgg caaacgtctg gctgatggta ttcgggggcg ctaaagatgc tggaagatct
     4921 gcgcgccaac agctggagtt tacgcccgtg ctgcatggtt cttgcctatc gcgtcgctca
     4981 tttttgttcg gtgtggcgca aaaagaacgt cctcaacaat atttgggcgg ccccgctgct
     5041 ggtgctgtat cgcattatca ccgaatgctt tttcggttat gaaatccagg cagccgcaac
     5101 cattggtcgc cgtttcacta tccatcacgg ttacgccgta gtgatcaata aaaacgtggt
     5161 agcgggggat gatttcacca ttcgccacgg cgtcactatc ggcaatcgtg gtgccgataa
     5221 catggcatgt ccacacattg gcaacggcgt cgagctcggt gccaacgtca ttattcttgg
     5281 tgatatcacg cttggtaaca acgtcaccgt gggcgcgggc agcgtggtgc tcgactctgt
     5341 cccggacaac gcgctggtgg tgggagaaaa agcgcgagtg aaggtaatca aatgaatatt
     5401 ctgcaattta atgtgcgact ggcggaaggc ggggcggcag gtgtggcgtt agatctccac
     5461 cagcgtgcgc tgcaacaggg gctggcgtca cattttgtct acggctacgg caaaggcggc
     5521 aaagagagcg tcagccatca gaactatccg caggtcatca aatatacgcc gcggatgacc
     5581 gcgatggcga atattgccct gtttcgtctg tttaatcgcg atctgtttgg caatttcaat
     5641 gagttatatc gcaccattac tcgtacaccg ggtccggtgg tcctgcattt tcatgtgctg
     5701 cacagctact ggctaaatct taagagcgtg gtgcgctttt gcgaaaaggt gaaaaaccac
     5761 aaaccggatg tcactctggt ctggacgctg cacgaccact ggagcgttac cgggcgctgc
     5821 gcctttaccg acggttgtga aggctggaaa acgggctgcc agaaatgccc gaccttaaat
     5881 aattatccgc cggtgaagat tgatcgggcg caccagctgg tggcgggcaa acgccagtta
     5941 ttccgtgaga tgctggcgct gggctgtcag tttatttccc ccagccagca tgtggctgac
     6001 gctttcaata gcctgtacgg tccagggcgt tgccggatta tcaataatgg cattgatatg
     6061 gcaacagaag cgattctggc ggatctgcct ccggtgcgcg aaactcaggg caagccgaaa
     6121 atcgcggtgg tggcgcatga cctgcgttac gacggcaaaa ctaaccagca actggtacgc
     6181 gagatgatgg cgctgggcga caaaatcgag ctgcatacct ttggtaagtt ctcgccgttc
     6241 accgctggca acgtggttaa tcacggcttt gaaactgaca agcgcaagtt gatgagcgcg
     6301 ctcaatcaga tggatgcgct ggtattcagt tctcgtgtcg ataactaccc actgattttg
     6361 tgtgaggcgt tatcaattgg cgtgccggtg attgccaccc atagcgacgc ggcgcgtgaa
     6421 gtgttgcaaa aatccggcgg taaaaccgtc agcgaagaag acgtgctgca actggtccag
     6481 ttaagcaaac cggaaatcgc gcaggcgata tttggtacca cgctggctga gttcagccaa
     6541 cgcagccgcg ccgcctacag tggacaacag atgctggagg agtatgtcaa cttctatcag
     6601 aatctgtagc tacctgctgc tgccgctgat ctacctgctg gttaacgtca aaatcgctca
     6661 gcttggcgaa agttttccca taaccatcgt cactttttta cctgtcttgt tactgctgtt
     6721 tttagaacgc atcagcgtta aaaaattgat gattgccctg gggattggcg cgggactcac
     6781 ggcgtttaac tatctgtttg gtcagtcgct ggatgccagt aaatacgtca cttcaaccat
     6841 gctgtttgtc tatattgtga tcattattgg catggtgtgg agtattcgct ttaaaacaat
     6901 ttcaccacac aaccatcgca agatattacg tttcttttat ctggtggtcg ggctggtggt
     6961 ggcgctggcg gcggtggaga tggcgcaaat tatcctcacc ggtggcagta gtattatgga
     7021 gtcgatttcg aaatatctga tttacagcaa cagctatgtg ctgaatttca tcaaattcgg
     7081 cggcaagcgc acgacagcac tttatttcga accggcattt ttcgctctgg cattaatctc
     7141 aatttggctc agcatcaaac agtttggtat caaaacgcct aaaaccgatg ctatgattct
     7201 cgcagggata atattatccg gatcgttttc aggggttatg acctttatcc tgttttattt
     7261 gctggagtgg gcatttcaat atctgaataa agaggcgatt aagaaaaagt taccgttagc
     7321 attgatttct ctggctgtat tcctggttgg tgtggtaatt gcatttcctt atatttccac
     7381 ccgtctgggc gatttaggta cggaaggatc gtcatcgtat tatcgtattg tcggtccgct
     7441 ggtgatggtc ggttattctt tgacccatat tgacggtgta gtcagatttg gctcacttta
     7501 tgaatatgtc gcatcattcg gaatatttaa cggtgcggat gtcggaaaaa ccatagacaa
     7561 tggtttgtat ctgctgatta tttatttctc ctggtttgcg gtgtttttat cgctgtggta
     7621 catggggaaa gtgataaaaa tgatgatcaa cgcttttggt gataaccgca attttcgcgt
     7681 gcaattatat ctttttactc cggtatcgct gttttttacc ggttcgatat ttagcccgga
     7741 atatgcattt ttaatcgtct gtccgtttat tttacgaaaa gcgttaaata tacaccctaa
     7801 ataattcgag ttgcaggaag ttggtgacgc agcgaatccc cagaagcgta cacgagtacg
     7861 tgactggggt gagcgaggaa aaccaacgca catgccgctt gaagtatgac gggtattacg
     7921 aggtaaaaca tgttgcttag cataatcact gtcgcgtttc gtaacctcga agggatagtc
     7981 aaaacacacg cctcgctggc gcatctggcg caggcggacg atatcagctt cgaatggatt
     8041 gttgtcgatg gcggttccaa tgacggcacc cgtgagtatc tggaaaatct caatggtatc
     8101 tataacctac gctttgtcag cgagccagat aacggtatct acgacgccat gaacaaaggc
     8161 attgcgatgg cacaaggcaa gttcgcgttg tttctcaact cgggcgatat ttttcatcag
     8221 gatgccgcat attttgtccg taagttaaaa atgcaaaaag ataacgtgat gatcaccggc
     8281 gatgcgctgc tggattttgg cgacgggcat aaaattaaac gtagcgccaa accgggttgg
     8341 tatatttatc acagcctgcc cgccagtcat caggcgatat ttttcccggt atccggtttg
     8401 aaaaaatggc gttatgacct ggaatataaa gtttcttctg attacgcgct ggcagccaaa
     8461 atgtataaag ccggttatgc atttaaaaaa ctcaatggcc tggtgtctga attttctatg
     8521 ggtggggtat ctaccaccaa taatatggaa ttgtgtgctg acgccaaaaa agtccaacgg
     8581 caaatattac atgtacctgg cttctgggca gaattatcct ggcatttacg ccagcgtact
     8641 acctcaaaga cgaaagcctt atataacaaa agctgaatat aagggaaaac gatgcaagat
     8701 ttaagtggat tctcggtgcc gaaagggttc cggggcggca acgctattaa agtgcaattg
     8761 tggtgggcag tacaggcaac attatttgcc tggtcgccac aagtattgta tcgctggcgg
     8821 gcatttttat tacgtttatt cggcgcaaaa ataggaaaaa acgtagttat tcgtccgtca
     8881 gtaaaaatta cctatccgtg gaaattaacc ttaggtgatt acgcgtgggt cggcgatgac
     8941 gtcaatttat ataccctcgg tgaaataacc attggcgcac attcggtgat atcgcaaaaa
     9001 agttatttat gcaccgggag ccacgaccat gcaagtcaac atttcaccat taacgccacg
     9061 cctattgtga ttggcgagaa atgctggctg gcaaccgatg tttttgttgc cccaggcgtc
     9121 acgatcggcg acggcaccgt cgtgggtgcg cgaagcagtg tttttaaatc gcttccggca
     9181 aatgtggttt gccgggggaa tcccgcagtg gtgatacgcg aacgcgttga aactgaataa
     9241 attcaaaaaa tacagaggaa taagacatgt caaaagtcgc tctcatcacc ggtgtaaccg
     9301 gacaagacgg ttcttacctg gcagagtttc tgctggaaaa aggttacgag gtgcatggta
     9361 ttaagcgtcg tgcatcgtca ttcaacaccg agcgcgtgga tcacatttat caggatccgc
     9421 acacctgcaa cccgaaattc catctgcatt atggcgacct gagtgatacc tccaacctga
     9481 cgcgcatttt gcgtgaagtg cagccggatg aagtctacaa cctgggggca atgagccacg
     9541 ttgcggtttc ttttgagtca ccggaatata ccgctgacgt cgacgcgatg ggtacgctgc
     9601 gcctgctcga ggcgatccgc ttcctcggtc tggaaaagaa aacccgtttc tatcaggctt
     9661 ccacctctga actgtacggt ctggtgcagg aaattccgca gaaagaaacc acgccgttct
     9721 atccgcgatc tccgtatgcg gtcgccaaac tgtacgccta ctggatcacc gttaactacc
     9781 gcgaatccta cggcatgtac gcctgcaacg gtattctctt caaccatgaa tccccgcgcc
     9841 gcggcgaaac cttcgttacc cgcaaaatca cccgcgcaat cgccaacatc gcccaggggc
     9901 tggaatcgtg cctgtacctc ggcaatatgg attccctgcg tgactggggc catgccaaag
     9961 actacgtaaa aatgcagtgg atgatgctgc aacaggaaca accggaagat ttcgttatcg
    10021 ctaccggcgt tcagtactcc gtacgtcagt tcgtggaaat ggcggcggca cagttgggca
    10081 tcaaactgcg ctttgaaggc acgggcgttg aagagaaggg cattgtggtt tccgtcaccg
    10141 gacatgacgc gccgggcgtt aaaccgggtg atgtgattat cgccgttgat ccgcgttact
    10201 tccgtccggc tgaagttgaa acgctgctcg gcgacccgac caaagcgcac gaaaaactgg
    10261 gctggaaacc ggaaatcacc ctcagagaga tggtgtctga aatggtggct aatgacctcg
    10321 aagcggcgaa aaaacactct ctgctgaaat ctcacggcta cgacgtggcg atcgcgctgg
    10381 agtcataagt atgagtagac aacgcatttt tattgctggc catcgcggga tggtcggttc
    10441 cgccatcagg cggcagctcg aacagcgcgg tgatgtggaa ctggtattac gcacccgcga
    10501 cgagctgaac ctgctggaca gccgcgccgt gcatgatttc tttgccagcg aacgtattga
    10561 ccaggtctat ctggcggccg cgaaagtggg cggcattgtt gccaacaaca cctatccggc
    10621 ggatttcatc tatcagaaca tgatgattga gagcaacatc attcacgccg cgcatcagaa
    10681 cgacgtgaac aaactgctgt ttctcggatc gtcctgcatc tacccgaaac tggcaaaaca
    10741 gccgatggca gaaagcgagt tattgcaggg cacgctggag ccgaccaacg agccttacgc
    10801 cattgccaaa atcgccggga tcaaactgtg cgaatcatac aaccgccagt acggacgcga
    10861 ttaccgctca gtcatgccga ccaacctgta tggcccgcat gacaacttcc acccgagtaa
    10921 ttcgcatgtg atccctgcat tgctgcgtcg cttccacgag gcgacggcac agaatgcacc
    10981 ggacgtggtg gtatggggca gcggtacacc gatgcgtgaa ttcctgcacg tcgatgatat
    11041 ggcggcggcg agcattcatg tcatggagct ggcgcatgaa gtttggctgg agaacaccca
    11101 gccgatgctg tcgcacatta acgtcggcac gggcgttgac tgcactatcc gcgagctggc
    11161 gcaaaccatc gccaaagtgg tgggttacaa aggtcgggtg gtttttgatg ccagcaaacc
    11221 ggatggcacg ccgcgcaaac tgctggatgt gacgcgcctg catcagcttg gctggtatca
    11281 cgaaatctca ctggaagcgg ggcttgccag cacttaccag tggttccttg agaatcaaga
    11341 ccgctttcgg gggtaatgat gtttttacgt caggaagact ttgccacggt agtgcgctcc
    11401 actccgcttg tctctctcga ctttattgtc gagaacagtc gcggcgagtt tctgcttggc
    11461 aaaagaacca accgcccggc gcagggttac tggtttgtgc cgggagggcg cgtgcagaaa
    11521 gacgaaacgc tggaagccgc atttgagcgg ctgacgatgg cggaactggg gctgtgtctg
    11581 ccgataacag caggccagtt ttacggtgtc tggcagcact tttatgacga taacttctct
    11641 ggcacggatt tcactactca ctatgtggtg ctcggttttc gcttcagagt cgctgaagaa
    11701 gatctgttac tgccggatga gcagcatgac gattactgct ggctgacgcc ggatgcgctg
    11761 ctcgccagtg acaatgttca cgctaacagc cgcgcctatt ttctcgctga gaagcgtgcc
    11821 ggagtacccg gattatgaaa atactggtct acggcattaa ctactcgccg gagttaaccg
    11881 gcatcggcaa atacaccggc gagatggtgg aatggctggc ggcacaaggt catgaggtgc
    11941 gggtcattac cgcaccgcct tactacccgc agtggcaggt gggcgagaac tattccgcct
    12001 ggcggtacaa acgagaagag ggggccgcca cggtgtggcg ctgtccgctg tatgtgccaa
    12061 aacagccgag caccctgaaa cgcctgttgc atctcggcag ttttgccgtc agcagtttct
    12121 ttcccctgat ggcgcaacgt cgctggaagc cggatcgcat tattggcgtg gtgccaacgc
    12181 tgttttgcac gccaggaatg cgcctgctgg cgaagctctc tggtgcgcgt accgtgctgc
    12241 atattcagga ttacgaagtg gacgccatgc tggggctggg ccttgccgga aaaggcaaag
    12301 gcggcaaagt ggcacagctg gcaacggcgt tcgaacgtag cggactgcat aacgtcgata
    12361 acgtctccac gatttcgcgc tcgatgatga ataaagccat cgaaaaaggc gtggcggcgg
    12421 aaaacgtcat tttcttcccc aactggtcgg aaattgcccg ttttcagcat gttgcagacg
    12481 ccgatgttga tgcccttcgt aaccagcttg gcctgccgga taacaaaaaa atcattcttt
    12541 actccggcaa tattggtgaa aagcaggggc tggaaaacgt tattgaagct gccgatcgtc
    12601 tgcgcgatga accgctgatt tttgccattg tcgggcaggg cggcggcaaa gcgcggctgg
    12661 aaaaaatggc gcagcagcgt ggactgcgca acatgcaatt ttttccgctg caatcgtatg
    12721 acgctttacc cgcactgctg aagatgggcg attgccatct ggtggtgcaa aaacgcggcg
    12781 cggcagatgc cgtattgccg tcgaaactga ccaatattct ggcggtaggc ggtaacgcgg
    12841 tgattaccgc agaggcccac acagaactgg ggcagctttg cgaaaccttt ccgggcattg
    12901 cggtttgcgt tgaaccggaa tcggtcgagg cgctggtggc ggggattcgt cagacgctcc
    12961 tgctgcccaa acacaacacg gtggcacgtg aatatgccga acgcacgctc gataaagaga
    13021 acgtgttacg tcaatttata aatgatattc ggggataatt atggcgcagt cgaaactcta
    13081 tccagttgtg atggcaggtg gctccggtag ccgcttatgg ccgctttccc gcgtacttta
    13141 ccccaagcag tttttatgcc tgaaaggcga tctcaccatg ctgcaaacca ccatctgccg
    13201 cctgaacggc gtggagtgcg aaagcccggt ggtgatttgc aatgagcagc accgctttat
    13261 tgtcgcggaa cagctgcgtc aactgaataa acttaccgag aacattattc tcgaaccggc
    13321 agggcgtaac actgcacctg ccattgcgct ggcggcgctg gcggcaaaac gtcatagccc
    13381 ggagagcgac ccgttaatgc tggtattggc ggcggatcat gtgattgccg atgaagacgc
    13441 gttccgcgcc gccgtgcgta acgccatgcc atatgccgaa gcgggcaagc tagtgacctt
    13501 tggcattgtg ccggatctac ctgaaaccgg atatggctat attcgtcgcg gtgaagtgtc
    13561 tgcgggtgag caggatacgg tggcctttga agtggcgcag tttgtcgaaa aaccgaatct
    13621 ggaaaccgcg caggcctatg tggcaagcgg cgaatattac tggaacagcg gcatgttcct
    13681 gttccgcgcc ggacgctatc tcgaagaact gaaaaaatat cgcccggata tcctcgatgc
    13741 ctgcgaaaaa gcgatgagcg ccgtcgatcc ggatctcgat tttattcgcg tggatgaaga
    13801 agcgtttctg tcctgcccgg aagagtcggt ggattacgcg gtcatggaac gtacggcaga
    13861 tgccgttgtg gtgccgatgg atgcgggctg gagcgatgtc ggttcctggt cttcattatg
    13921 ggagatcagc gcccacaccg ccgagggcaa cgtatgccac ggcgatgtga ttaatcacaa
    13981 aactgaaaac agctatgtgt acgccgaatc tggcctggtc accaccgtcg gggtgaaaga
    14041 tctggtggtg gtgcagacca aagatgcggt gctgattgcc gatcgtaacg cggtgcagga
    14101 tgtgaaaaaa gtggtcgagc agatcaaagc cgatggtcgc catgagcatc gggtacatcg
    14161 cgaagtgtat cgtccgtggg gcaaatatga ctctatcgac gcgggcgacc gctaccaggt
    14221 gaaacgcatc accgtgaaac cgggcgaagg gttgtcggta cagatgcacc atcaccgcgc
    14281 ggaacactgg gtagtggtcg cgggaacggc aaaagtcacc attgacggtg atatcaaact
    14341 gcttggtgaa aacgagtcca tttatattcc gctgggggcg acgcactgct tggaaaaccc
    14401 ggggaaaatt ccgctcgatt taattgaagt gcgctccggc tcttatctcg aagaggatga
    14461 tgtggtgcgc ttcgcggatc gctacggacg ggtgtaaacg tcgcatcagg cgctatttgt
    14521 cggatgcggc gtgaacgtct tatccgacct acggttcggg tttgtaggcc tgataagacg
    14581 cggcagcgtc gcatcaggca atgaatgcga aaccgcggtg taaataacga caaaaacaaa
    14641 attggccgtt tcggtcaggg ccaactattg cctgaaaaag ggtaacgata tgaaaaaatt
    14701 aaactgcttt aaagcctatg atattcgcgg gaaattaggc gaagaactga atgaagatat
    14761 tgcctggcgt attggtcgcg cctatggcga atttcttaaa ccgaaaacca ttgttttagg
    14821 cggcgatgtc cgcctcacca gcgaaacctt aaaactggcg ctggcgaaag gtttacagga
    14881 tgcgggcgtc gatgtgctgg atattggcat gtccggcacc gaagagattt atttcgccac
    14941 gttccatctc ggcgtggatg gcggcatcga agttaccgcc agccataacc cgatggatta
    15001 taacggcatg aaactggtgc gcgaaggggc tcgcccgatc agcggtgata ccggactgcg
    15061 cgacgtccag cgcctggcag aagccaacga cttccctccc gttgatgaaa ccaaacgcgg
    15121 tcgctatcag caaatcaacc tgcgtgacgc ttacgttgat cacctgttcg gttatatcaa
    15181 tgtcaaaaac ctcacgccgc tcaagctggt gatcaactcc gggaacggcg cagcgggtcc
    15241 ggtagttgac gccatcgaag cccgctttaa agctctcggc gcacctgtgg aattgatcaa
    15301 agtgcacaac acgccggacg gtaatttccc caacggtatt cctaacccgc tactgccaga
    15361 atgccgcgac gacacccgca atgcggtcat caaacacggc gcggatatgg gcattgcctt
    15421 tgatggcgat tttgaccgct gcttcctgtt tgacgaaaaa gggcagttta tcgaaggcta
    15481 ctacattgtc ggcctgctgg cagaagcgtt cctcgaaaaa aatcccggcg cgaagatcat
    15541 ccacgatcca cgtctctcct ggaacaccgt tgatgtggtg actgccgcag gcggcacccc
    15601 ggtaatgtcg aaaaccggac acgcctttat taaagaacgt atgcgcaagg aagacgccat
    15661 ctacggtggc gaaatgagcg cccaccatta cttccgtgat ttcgcttact gcgacagcgg
    15721 catgatcccg tggctgctgg tcgccgaact ggtgtgcctg aaaggaaaaa cgctgggcga
    15781 actggtacgc gaccggatgg ctgcgtttcc ggcaagcggt gagatcaaca gcaaactggc
    15841 gcaacccgtt gaggcgatta accgcgtgga acagcatttt agccgtgagg cgctggcggt
    15901 ggatcgcacc gatggcatca gcatgacctt tgccgactgg cgctttaacc tgcgctcctc
    15961 caacaccgaa ccggtggtgc ggttgaatgt ggaatcgcgc ggtgatgtgc cgctgatgga
    16021 agcgcgaaca cgaactctgc tgacgttgct gaacgagtaa tgtcggatct tcccttaccc
    16081 cattgcgggt aaggggcaaa taacaggaac aacgatgaca aatctaaaaa agcgcgagcg
    16141 agcgaaaacc aatgcatcgt taatctctat ggtgcaacgc ttttcagata tcaccatcat
    16201 gtttgccggg ctatggctgg tttgcgaagt gagcggactg tcattcctct acatgcacct
    16261 gttggtggcg ctgattacgc tggtggtgtt ccagatgctg ggcggcatca ccgattttta
    16321 tcgctcatgg cgcggtgttc gggcagcgac agaatttgct ctgttgctac aaaactggac
    16381 cttaagcgtg attttcagcg ccggactggt ggcgttcaac aatgatttcg acacgcaact
    16441 gaaaatctgg ctggcgtggt atgggctgac cagtatcgga ctggtggttt gccgttcatg
    16501 tattcgcatt ggggcgggct ggctgcgtaa tcatggctat aacaagcgca tggtcgcggt
    16561 ggcgggggat ttagccgccg gacaaatgct gatggagagc ttccgtaatc agccgtggtt
    16621 agggtttgaa gtggtgggcg tttaccacga cccgaaaccg ggcggcgttt ctaacgactg
    16681 ggcgggtaat ctgcaacaac tggtcgagga tgcgaaagcg ggcaagattc ataacgtcta
    16741 tatcgcgatg caaatgtgcg acggcgcgcg agtgaaaaaa ctggtccatc aactggcgga
    16801 caccacctgt tcggtgctgc tgatccccga cgtctttact ttcaacattc tccattcacg
    16861 tctcgaagag atgaacggcg ttccggtggt gccgctgtat gacacgccac tttccggggt
    16921 taaccgcctg ctcaaacgtg cggaagacat tgtgctggcg acgcttatcc tgctgctgat
    16981 ctccccggtg ctgtgctgca ttgcgctggc ggtgaaactc agttcacctg ggccggttat
    17041 tttccgccag actcgctacg gcatggatgg caagccgatc aaagtgtgga agttccgttc
    17101 catgaaagtg atggagaacg acaaagtggt aactcaggcg acgcagaacg atccgcgcgt
    17161 caccaaagtg gggaactttc tgcgccgcac ctcgctggat gaactgccgc agtttatcaa
    17221 tgtgctgacc ggggggatgt cgattgtcgg tccacgtccg cacgcggtgg cgcataacga
    17281 acagtatcga cagctcattg aaggctacat gctgcgccat aaagtgaaac cgggcattac
    17341 cggttgggcg cagattaacg gctggcgcgg cgaaaccgac acgctggaga aaatggaaaa
    17401 acgcgtcgag ttcgaccttg agtacatccg cgaatggagc gtctggttcg atatcaaaat
    17461 cgttttcctg acggtattca aaggattcgt taacaaagcg gcatattgat atgaacttac
    17521 gtgaaaaaac catcagcggc gcgaagtggt cggcgattgc cacggtgatc atcatcggcc
    17581 tcgggctggt gcagatgacc gtgctggcgc ggattatcga caaccaccag ttcggcctgc
    17641 tgactgtgtc gctggtgatt atcgcgctgg cagatacgct ttctgacttc ggtatcgcta
    17701 actcgattat tcagcgaaaa gaaatcagcc accttgaact caccacgctg tactggctga
    17761 acgttgggct ggggcttgtg gtgtgcgtgg cggtgttttt gttgagtgat ctcatcggcg
    17821 acgtgctgaa taacccggac ctggcaccgt tgattaaaac attgtcgctg gcgtttgtgg
    17881 tgatccccca cgggcagcag ttccgcgcat tgatgcaaaa ggaactggag ttcaacaaaa
    17941 tcggcatgat cgaaaccagc gcggtgctgg cgggctttac ttttacggtg gttagcgccc
    18001 atttctggcc gctggcgatg accgcgatcc tcggttatct ggtcaatagt gcggtaagaa
    18061 cgctgctgtt tggctacttt ggccgcaaaa tttatcgccc cggtctgcat ttctcgctgg
    18121 cgtcggtggc accgaactta cgctttggtg cctggctgac ggcggacagc atcatcaact
    18181 atctcaatac caacctttcg acgctggtgc tggcgcgtat tctcggcgcg ggcgtggcag
    18241 ggggatacaa cctggcgtac aacgtggccg ttgtgccacc gatgaagctg aacccaatca
    18301 tcacgcgcgt gttgtttccg gcgtttgcca aaattcagga cgataccgaa aagctgcgcg
    18361 tcaacttcta caagctgctg tcggtggtgg ggattatcaa ctttccggcg ctgctcgggc
    18421 tgatggtggt gtcgaataat tttgtaccgc tggtctttgg tgagaagtgg agcagcatta
    18481 ttccggtgct gcaattgctg tgtgtggtgg ggctactgcg ctccgtaggt aacccgattg
    18541 gctcgctact gatggcgaaa gcgcgggtcg atatcagctt taaattcaac gtattcaaaa
    18601 cctttctgtt tattccggcg attgttatag gtgggcagat ggcgggcgca atcggcgtca
    18661 cgctcggctt cctgctggtg cagattatca acaccattct gagttacttc gtgatgatta
    18721 aaccggttct tggttccagt tatcgccagt acatcctgag tttatggctg ccgttttatc
    18781 tctcgctgcc gacgctggtg gtcagttatg cgctgggcat tgtgctgaaa gggcaactgg
    18841 cgctggggat gctgctggcg gtgcaaatag ccacgggtgt gctggcgttt gtggtgatga
    18901 ttgtgctgtc gcgccatccg ctggtggtgg aagtgaagcg tcagttttgc cgcagcgaaa
    18961 aaatgaaaat gcttttacgg gcggggtgag ttgttgtttt cggttccgac aacggttgcc
    19021 gggtgcagcg taaaacgcct tatccatcct acggttctgt gctcgggacc tgtaggcctg
    19081 ataagacgcg tcagcgtcgc atcaggcact gaatgccgga tgcggcgtga acgccttatc
    19141 cggcctacgg ttctgtgctc gggtcttgta ggcctgataa gacgcgccag cgtcgcatca
    19201 ggcaaaaaac ggcaacgaac acaacaaaca cagcctcacc tggcaccaaa tttatgagga
    19261 aaaaatgaaa ttacttattc tgggcaacca cacttgcggc aatcgtggcg acagcgccat
    19321 cttgcgcggc ttacttgatg ccatcaacat cctcaatcca cacgccgaag tggacgtgat
    19381 gagccgctat ccggtcagtt cttcctggct gctcaaccgc ccggtaatgg gcgatccgct
    19441 gttcctgcaa atgaaacaac acaacagcgc ggcgggcgtt gtcgggcgcg ttaaaaaagt
    19501 ccttcgtcgc cgctaccagc atcaggtatt gctctcacgc gtcaccgaca ctggcaagct
    19561 gcgtaatatc gccatcgccc agggattcac cgacttcgtg cgcctgctgt caggttacga
    19621 cgccattatc caggtcggcg gatcgttttt tgtcgatctc tatggcgtgc cgcagtttga
    19681 acatgcgctt tgcacattta tggcgaaaaa gccgctgttt atgattggtc acagcgtcgg
    19741 ccccttccag gatgagcaat ttaaccaact ggcgaactac gtttttggtc actgtgacgc
    19801 gctgatcctg cgcgaatcgg tcagcctcga tctaatgaaa cgcagcaata tcaccactgc
    19861 aaaagttgaa catggcgtcg ataccgcgtg gctggtcgat caccacacag aagacttcac
    19921 cgccagctat gccgtccaac actggctgga cgttgccgca caacagaaaa cggtggccat
    19981 taccctgcgc gaactggcac cgttcgacaa acgcctcggc accactcaac aagcgtatga
    20041 aaaagccttt gccggggtgg tcaatcgcat tctcgacgaa gggtatcagg tgattgcgct
    20101 ctccacctgt acgggcatcg acagctataa caaagacgac cgcatggtgg cgctcaacct
    20161 gcgccagcat atcagcgatc ctgcccgtta ccacgtggtg atggatgaac tcaacgatct
    20221 ggaaatgggc aaaattctcg gtgcctgtga actcaccgtc ggtacgcgcc tgcactcggc
    20281 cattatctca atgaactttg ctactccggc gattgccatc aactatgaac ataaatccgc
    20341 cgggatcatg cagcagctgg ggctgccaga gatggcgatt gatatccgtc atttattaga
    20401 cggcagcctg caagtgatgg ttgcggatac cttaggccaa cttccggcgc tgaacgcacg
    20461 acttagcgaa gctgtcagtc gtgagcgtca gacaggaatg cagatggtgc agtccgtact
    20521 ggaacgcatt ggggaggtaa aatgaaggtc ggcttcttct tattgaaatt cccgctgtcg
    20581 tcggaaacct tcgttcttaa ccaaatcact gcgtttattg atatgggctt tgaagtggag
    20641 attgtcgcgc tgcaaaaagg cgacacacaa aacacccacg cggcatggac gaaatacaac
    20701 cttgccgcca gaacccgctg gttacaggac gaacctacgg gcaaagtggc gaaactgcgc
    20761 caccgcgcca gccagacctt acgcggcatt catcgtaaaa atacctggca ggcgctcaac
    20821 ctcaaacgct atggcgccga gtcgcggaac ctgattttgt ctgccatttg tggtcaggtc
    20881 gcaacaccat tttatgccga tgtctttatc gctcattttg gccctgcggg ggtaaccgca
    20941 gcaaaactac gcgaactggg tgtcattcgc ggcaaaattg ccactatctt ccacggtatt
    21001 gatatctcca gtcgggaagt gctcaaccac tacactcccg aatatcaaca actgtttcgc
    21061 cgtggcgacc tgatgttacc gataagcgat ctgtgggccg gaaggctgca aaaaatgggc
    21121 tgcccgaggg aaaaaatcgc cgtatcgcgc atgggcgtag atatgacgcg ctttagcccg
    21181 cgtcccgtga aagcgcccgc aacaccgctg gagattattt ccgtcgcacg cttaaccgag
    21241 aaaaaaggtc tgcatgtggc gattgaagcc tgccggcagt tgaaagagca gggcgtggca
    21301 tttcgctatc gcatcctcgg cattggcccg tgggaacgac gcctgcgcac gctcatcgaa
    21361 caatatcaac tggaagatgt ggtggagatg ccgggcttta aaccgagcca tgaagtgaaa
    21421 gcgatgctcg acgacgcgga tgtcttcctg ttgccatcgg ttacaggtgc ggatggcgat
    21481 atggaaggca ttccggtagc gctgatggaa gcgatggcgg tcggcattcc ggtggtttct
    21541 actctgcata gcggaatacc ggaactggtg gaggccgata aatccggctg gctggtgcct
    21601 gagaacgatg ctcgcgcact ggcgcaacgt ctggcggcat ttagccaact ggacactgac
    21661 gaactggctc cggttgtcaa acgtgctcgc gaaaaagtcg aacacgattt taaccagcag
    21721 gtgattaatc gagaactcgc cagcttgtta caggctttat agaggagaac gtatgccatt
    21781 taaaacactc tcccgacgca ccttcctgac ggcaagttcg gcgcttgcct tcctccatac
    21841 ccctttcgct cgcgcacttc ccgcccgaca aagcgttaac attaacgact acaaccctca
    21901 cgactggatc gcctcattta aacaagcctt cagcgaagtg caaacggtcg tcgtgcctgc
    21961 cggattggtt tgtgacaata tcaacaccgg catcttcatc cctcccggta aaacgttaca
    22021 catccttgga agcctgcgcg gcaacggcag agggcgattt gtcttacagg acggcagcca
    22081 ggtgacaggg gaggagggcg gcagtatgca taacatcacc ctggatgtgc gcggctctga
    22141 ctgcaccatc aaagatctgg cgatgagcgg ctttggcccg gtaacgcaga tttatatcgg
    22201 cggcaaaaac aaacgggtca tgcgcaacct gaccatcgat aacctcactg tcagccacgc
    22261 taattacgcc atcttacgcc agggatttca taatcagatt atcggtgcca acatcaccaa
    22321 ctgtaagttc agcgacttac agggggacgc tattgaatgg aacgtggcga ttaacgacag
    22381 tgatattttg atctccgacc atgtcatcga gcgcatcaac tgtaccaacg gcaaaatcaa
    22441 ctggggcatc ggtataggcc ttgcgggaag cacttacgat aataattacc cggaagacca
    22501 ggcagtgaaa aactttgtcg tggcaaatat cacgggatcg gattgtcggc agttgatcca
    22561 tgttgaaaat ggtaaacatt ttgttattcg taatatcaaa gcccgcaata tcacgccgga
    22621 tttcagtaag aaagcaggta ttgataacgc gacagttgct atttacggtt gtgacaattt
    22681 cgtgattgat aatattgaaa tgattaatag cgccgggatg ttaatcggtt atggggtaat
    22741 taaaggcaaa tatctctcga taccgcaaaa tttccgagtg aataatattc aactggataa
    22801 cacccatctt gcttataaat tgcgcggcat ccaaatctcc gccgggaatg ctgcctcctt
    22861 tgtggcactg actaacattg agatgaagcg tgcctcgctg gagcttcaca ataaaccgca
    22921 acatcttttt atgcgtaata tcaaagtgat gcaggaatcc tcagttggtc ccgcattgag
    22981 catgaacttc gacatgcgca aagacgttcg cggcgtcttt atggcgaaaa aagaaacact
    23041 gctgtctctt gcaaatgttc atgcggtgaa tgaaaaaggg caaagctccg tcgatatcga
    23101 cagagttaat caccatattg ttaatgtgga aaagattaac tttagattgc cagaacggag
    23161 ggagtagatt tg
//
LOCUS       DAZUCE010000064        22813 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZUCE010000064 DAZUCE010000000
VERSION     DAZUCE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.064"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            173..856
                     /locus_tag="ACHY03_002062"
     CDS             173..856
                     /locus_tag="ACHY03_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFS3905294.1"
                     /translation="MNLKKIFFSAATVSVLCTLTGCDYIEEGKPESSLLKQQEEHNNK
                     IELIEKQQAQLKSQLETIQKQQTGIITSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSIPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            900..4117
                     /locus_tag="ACHY03_002063"
                     /pseudo
     CDS             900..4117
                     /locus_tag="ACHY03_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            4544..6199
                     /gene="lldP"
                     /locus_tag="ACHY03_002064"
     CDS             4544..6199
                     /gene="lldP"
                     /locus_tag="ACHY03_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFS3905295.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6199..6975
                     /gene="lldR"
                     /locus_tag="ACHY03_002065"
     CDS             6199..6975
                     /gene="lldR"
                     /locus_tag="ACHY03_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFS3905296.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            6972..8162
                     /gene="lldD"
                     /locus_tag="ACHY03_002066"
     CDS             6972..8162
                     /gene="lldD"
                     /locus_tag="ACHY03_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFS3905297.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            8317..8790
                     /gene="trmL"
                     /locus_tag="ACHY03_002067"
     CDS             8317..8790
                     /gene="trmL"
                     /locus_tag="ACHY03_002067"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFS3905298.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYSAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(8843..9664)
                     /gene="cysE"
                     /locus_tag="ACHY03_002068"
     CDS             complement(8843..9664)
                     /gene="cysE"
                     /locus_tag="ACHY03_002068"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFS3905299.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(9744..10763)
                     /gene="gpsA"
                     /locus_tag="ACHY03_002069"
     CDS             complement(9744..10763)
                     /gene="gpsA"
                     /locus_tag="ACHY03_002069"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFS3905300.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(10763..11230)
                     /gene="secB"
                     /locus_tag="ACHY03_002070"
     CDS             complement(10763..11230)
                     /gene="secB"
                     /locus_tag="ACHY03_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFS3905301.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11293..11544)
                     /gene="grxC"
                     /locus_tag="ACHY03_002071"
     CDS             complement(11293..11544)
                     /gene="grxC"
                     /locus_tag="ACHY03_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFS3905302.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(11686..12117)
                     /locus_tag="ACHY03_002072"
     CDS             complement(11686..12117)
                     /locus_tag="ACHY03_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFS3905303.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12362..13906
                     /gene="gpmM"
                     /locus_tag="ACHY03_002073"
                     /gene_synonym="pgmI"
     CDS             12362..13906
                     /gene="gpmM"
                     /locus_tag="ACHY03_002073"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFS3905304.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQSDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            13940..15199
                     /gene="envC"
                     /locus_tag="ACHY03_002074"
     CDS             13940..15199
                     /gene="envC"
                     /locus_tag="ACHY03_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFS3905305.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15203..16162
                     /locus_tag="ACHY03_002075"
     CDS             15203..16162
                     /locus_tag="ACHY03_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFS3905306.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(16149..17183)
                     /gene="waaH"
                     /locus_tag="ACHY03_002076"
     CDS             complement(16149..17183)
                     /gene="waaH"
                     /locus_tag="ACHY03_002076"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFS3905307.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17422..18447)
                     /gene="tdh"
                     /locus_tag="ACHY03_002077"
     CDS             complement(17422..18447)
                     /gene="tdh"
                     /locus_tag="ACHY03_002077"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFS3905308.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18457..19653)
                     /gene="kbl"
                     /locus_tag="ACHY03_002078"
     CDS             complement(18457..19653)
                     /gene="kbl"
                     /locus_tag="ACHY03_002078"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFS3905309.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            19867..20799
                     /gene="rfaD"
                     /locus_tag="ACHY03_002079"
     CDS             19867..20799
                     /gene="rfaD"
                     /locus_tag="ACHY03_002079"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFS3905310.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            20809..21855
                     /gene="rfaF"
                     /locus_tag="ACHY03_002080"
     CDS             20809..21855
                     /gene="rfaF"
                     /locus_tag="ACHY03_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFS3905311.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYLQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            21859..>22813
                     /gene="rfaC"
                     /locus_tag="ACHY03_002081"
     CDS             21859..>22813
                     /gene="rfaC"
                     /locus_tag="ACHY03_002081"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFS3905312.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPLKSLNNLEAAAVYKEITL"
BASE COUNT         5663 a         5873 c         5960 g         5317 t
ORIGIN      
        1 acaaacacag acatcttttg aaacgaatga tacattttta tgatgaatgc ttaaaacaat
       61 atttaatcga cacccatcac tgtcattaat gtaaagcaac atagatcacc aataatacaa
      121 tggacaactc taccggcatt gatcgctggt cggaggcaat aaggattttt acatgaactt
      181 gaagaagatt tttttcagtg ctgccactgt ttcagttctc tgcacattaa cgggatgtga
      241 ctacattgaa gaaggcaagc cagaaagtag tttactgaaa caacaagaag aacataacaa
      301 taaaattgag ctaattgaaa aacaacaagc acagctaaag agtcaactgg aaactattca
      361 aaaacaacaa actggcataa taaccagtac aaagacttta actcacgtaa ttaaatccgt
      421 taaagatcaa caaaacactt ttatttttac cgagtttaat ccggcaaaaa cgaagtattt
      481 catcctgaac aatggttcgg tggctttagc gggccgagtg ttatccattg acgcaacaga
      541 gaatggcagt gttattcata tttcactggt caacttatta agtataccaa tctcaaacat
      601 tggttttaat gcgacatggg gtggtgaaaa acctgtcgat gccaaagagt ttgcccggtg
      661 gcaacaatta cttttcaaca catcaatgaa atccacattg aaattattac caggtcaatg
      721 gcaagacatt aatttgacac tgaagggtgt atcgccgaat aacctgggat atctgaagtt
      781 agccatcaat atggagaata ttcagtttga caaccttccc tctgctgaaa atcggcagaa
      841 aagaagcaaa aaataaatgc agcttctgcg cttatatcaa ggaatagaga acatcattaa
      901 tgaacaaaat atttaaagtt atctggaacc ctgcgacagg gaattatact gttaccagcg
      961 aaacggcaaa aagccgtggc aagaaatccg ggcgcagtaa gctgttaatt tctgccgtgg
     1021 ttgcgggtgg gttgttgtcg tcgtttgagg cactggcaac aggtaatgaa cctggtacac
     1081 ctgttggcac aggatctggc tgggttgcta taggccagga atcacaggca aatgctttta
     1141 ccgatggtaa tggtgccagt actgcagtgg gttataaagc tttcgctgaa ggaacatgga
     1201 gttccgctat tggcgccatg acccatgcaa ttggtccggc ttcaatggca ttcggggtta
     1261 atgccgtctc aaccggtcaa cgaagtatcg ctatgggcgc atcgtcaagt tcagtgggtc
     1321 aatattcgat ggcacttggg cgatatgcga attcgggtgg tgattattcg atcgcgcaag
     1381 gggataaatc caatgctgct ggtctgaatg ccattgcagt gggaagaagc agtagtgcgg
     1441 caggcgagaa agccactgcg ctgggcaacg cagctaaagc tactgagatt atgagtattg
     1501 ctcttggcga cagcgccaat gcgtcaaaag cgtattcaat ggcgctggta gcaagtagcg
     1561 tcgcatctga agaaaacgca attgccctgg ggcgtagcag ttagctagcg gtactgacag
     1621 cctcgcatta ggcagacaaa cacttgccag tacagcgaac gctattgcga taggtgctga
     1681 gagcaaagcc gctgaaaatg cgacggccgt tggtaccaaa gcggaggcca atggacttaa
     1741 tagtatcgcg ttgggctctg ggggtattgc agatgccgat aacacgattg ctctgggcaa
     1801 tcaaagcctg gcagtagctg ctggtgcaat tgccatcggt caggggaata aagctgacgg
     1861 cgcaaatgct atcgcgctgg gtaatggtag tgttacaggt ggcgcaaatg ctattgctct
     1921 tggacaaggt agttatgccg gtttagaaaa tggcattgca attggtggtc aagccagtgc
     1981 tcaagggaca aattccgttg ctctgggtgc gggttctgta gcgactgaag ataattccgt
     2041 ctctgtgggt aacacaacag atcagcgaaa aattgtcaat atggcagcag gtgatatcag
     2101 cactagcagt actgatgcca taaatggatc acagctttat gctatcagta agtcagtagc
     2161 ggaccgtctt ggtggggggg ctaccgtcaa tgcgcaaggt gtcgttactg ccccgaatta
     2221 cagactgaaa aatggtattt atggcaatgt tggcgatgct ttagcggatc taaacaataa
     2281 cacgatacaa tgggactccg ttaaaaaggg atatagtgcg gcacatggta cagatgccac
     2341 cagcaccatc accaacgtta aagccggcac tatttctgct accagtacgg atgcggttaa
     2401 cggttctcag ttgaatgcga cgaatatgat gattgagcag aacagccaaa tcatcaatca
     2461 gcttgctggt aacaccgacg caacctatat cgaagaaaat ggcgctggta tcaactacgt
     2521 tcgtactaac gacaacggtt tagcctttaa cgatgcaagc gcctctggtg tcggcgctac
     2581 agctgtaggt tataacgctg tcgcgtctgg tgccagcagc gtagccattg gtcagaacag
     2641 caatagcacc gttgataccg gtattgcgct gggtagcagc tccgtttcca gccgtgtgat
     2701 tgccaaaggt tctcgtgaca ctagcgtaac ggaaaatggc gtggtgattg gttatgacac
     2761 cactgacggc gaactgctag gtgcattgtc aattggtgat gacggtaaat accgccaaat
     2821 catcaacgta gctgatggtt ccgaagccca tgacgccgtt acggttcgtc aattgcagaa
     2881 tgcgattggt gcggtcgcaa ccacgccaac caagtacttt cacgccaact caacggcaga
     2941 agactcactg gccgttggtg aagactcgct ggcaatgggt gcgaaaactg tcgttaatgg
     3001 taatgcaggt attggtatcg gtttgaacac tctggttctg gctgatgcga tcaacggcat
     3061 tgctatcggc agtaacgcac gcgcaaacca tgcaaacagt attgcaatgg gtaacggttc
     3121 tcagaccacc cgtggtgcac agactggcta caccgcctac aacatggacg caccgcagaa
     3181 ctctgtaggt gagttttctg ttggtagtga agacggtcaa cgtcagatca ccaacgtcgc
     3241 agctggttca gcggataccg atgcggtcaa cgtaggtcag ttgaaagtaa cggatgcgca
     3301 ggtttcccag aatacccaga gcattactaa cctgaacaat caggtcacta atctggatac
     3361 tcgcgttact aatatcgaaa acggtattgg cgacatcgta accaccggta gcaccaagta
     3421 cttcaagact aacaccgatg gcgtagatgc caatgcccag ggtaaagata gcgttgctat
     3481 tggttctggt tccattgctg ccgctgacaa cagcgtcgct ctgggtacag gctccgtggc
     3541 caacgaagaa aacacgatct ctgtgggttc ttctaccaac caacgccgta tcaccaacgt
     3601 agctgccggt gttaatgcca ccgatgcggt taacgtgtcg cagctgaaat cttctgaagc
     3661 aggcggcgtg cgctacgaca ccaaagctga tggttctatc gactatagca atatcaccct
     3721 cggtggtggc aacggcagta cgactcgtat cagcaacgtc tccgctggcg tcaacaacaa
     3781 cgacgcggtg aactacgcgc agttgaagca aagcgtgcag gaaacgaagc aatacaccga
     3841 tcagcggatg gttgagatgg ataacaaact gtctaaaact gaaagcaagt tgagcggtgg
     3901 tatcgcttct gcaatggcaa tgaccggtct gccgcaggct tacactccag gtgccagcat
     3961 ggcctctatt ggtggcggta cttacaacgg tgaatcggct gttgctttag gtgtatcgat
     4021 ggtgagcgcc aatggtcgtt gggtctacaa attacaaggt agtaccaata gccagggtga
     4081 atactccgcc gcactcggtg ccggtattca gtggtaatga tccattaagt tagtgtgact
     4141 aagggcaaac gccaaattgc ctgatgcgct acgcttatca ggcctacagg gtgattctgc
     4201 acattcctat aggccgggta aggtgttcac gccgcatctg gcaagataag gcgctccggg
     4261 tcaacaatct aagggcaatt ctctgatgag gattgccctt ttctttacca gacatctccc
     4321 cccacaagaa ttggccctac caattcttcg cttatctgac ctctggttca caattcccca
     4381 attaaaactc acatcaatgt tgccaataca taacatttag ttaactattc attgtcatta
     4441 tccctacaca acacaattgg cagtgccact tttacacaac gtgtgacaag gagatgagca
     4501 acagactcat tacacgatgt gcgtggactc caggagacct gcaatgaatc tctggcaaca
     4561 aaactacgat cccgccggga atatctggct ttccagcctg atagcatcgc ttcccatcct
     4621 gtttttcttc tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac
     4681 ggtggcaatc gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct
     4741 ggcctcggtg gtttatggct tcttctatgg tttgtggccc atcgcgtgga ttattattgc
     4801 agcagtgttc gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc
     4861 tattctttcg ataaccccag accagcgcct gcaaatgctg atcgtcggtt tctgtttcgg
     4921 cgcgttcctc gaaggagctg caggctttgg cgcaccggta gcaattaccg ccgcattgct
     4981 ggtcggtctg ggctttaaac cactgtacgc cgccgggctg tgcctgattg ttaacaccgc
     5041 gccagtggca tttggcgcga tgggcattcc aatcctggtt gccggacagg taacaggtat
     5101 cgacagcttt gagattggtc agatggtggg gcggcagcta ccgtttatga ccattatcgt
     5161 gctgttctgg atcatggcga ttatggacgg ctggcgcggt atcaaagaga cgtggcctgc
     5221 ggtcgtggtt gcgggcggct cgtttgccat cgctcagtac cttagctcta acttcattgg
     5281 gccggagctg ccggacatta tctcttcgct ggtatcactg ctctgcctga cgctgttcct
     5341 caaacgctgg cagccagtgc gcgtattccg ttttggtgat ttgggtgcat cgcaggttga
     5401 tatgacgctg gctcacaccg gttacacggc gggtcaggtg ctgcgtgcct ggacaccgtt
     5461 cctgttcctg accgccaccg taacactgtg gagtatcccg ccatttaaag ccctgttcgc
     5521 ttcgggcggc gcgctttatg aatgggtgat caacattccg gtgccgtatc tcgacaaact
     5581 ggttgctcgt atgccgccgg tcgtcagcga agctaccgct tacgccgccg tatttaagtt
     5641 tgactggttc tctgccactg gcaccgccat tctgtttgct gcactgctct caattgtttg
     5701 gctgaagatg aaaccgtctg acgctatcag caccttcggc agcacactga aagaactggc
     5761 cctgcccatc tactctatcg gtatggtgct ggcgttcgcc tttatctcta actattccgg
     5821 gctgtcatca acactggcgc tggcactggc gcacaccggt catgctttta ccttcttctc
     5881 accgttcctt ggttggctgg gggtattcct gaccgggtcg gatacgtcat ctaacgccct
     5941 gttcgccgcg ctgcaagcca ccgcagcaca acaaattggc gtctctgatc tgttgctggt
     6001 tgccgccaat accaccggtg gcgtcaccgg taagatgatc tccccgcaat ctatcgctat
     6061 cgcctgtgcg gcggtaggcc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa
     6121 acacagcctg atcttcacct gtatggtggg cgtgatcacc acgcttcagg cttatgtctt
     6181 aacgtggatg attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat
     6241 cgtgtgcggg cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgaa
     6301 cgtcaactgg cgatgcaact cggcgtatca cgtaattcac tgcgcgaggc gctggcaaaa
     6361 ctggtgagcg aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat tcgctggcgt
     6421 catgacacat ggtcggagca aaacatcgtc cagccgctaa aaacgctgat ggccgatgat
     6481 ccggattaca gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg
     6541 catgcggcaa tgcgcgccac acctggcgac aaagaaaaga ttcagctttg ctttgaagca
     6601 acgctaagtg aagacccgga tatcgcctca caagcggacg ttcgttttca tctggcgatt
     6661 gccgaagcct cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg
     6721 caatcctcag tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt tttttcacaa
     6781 ctgaccgaac aacatcaggc tgtcattgac gccatttttg ctggtgatgc cgacggggcg
     6841 cgtaaagcaa tgatggcgca ccttagtttt gttcacacca caatgaaacg attcgatgaa
     6901 gatcaggctc gccacgcacg gattacccgc ctgcccggtg agcataatga gcattcgagg
     6961 gagaaaaacg catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc
     7021 tgccgccgtt cctgttccac tatatggatg gtggtgcata ttctgaatac acgctgcgcc
     7081 gcaacgtgga agatttgtca gaagtggcgc tgcgccagcg tattctgaaa aacatgtccg
     7141 acttaagcct ggaaacgacg ctgtttaatg agaaattgtc gatgccggtg gcgctaggtc
     7201 cggtaggttt gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc aaagcggcgg
     7261 acgcgcatgg cattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtcg
     7321 cgccagccat caagcgccca atgtggttcc agctttatgt actgcgcgat cgcggcttta
     7381 tgcgtaacgc gctggagcga gcaaaagcag cgggttgttc gacgctggtt tttaccgtgg
     7441 atatgccgac accgggcgca cgctaccgtg atgcgcattc aggtatgagc ggcccaaatg
     7501 cggcaatgcg ccgctacttg caagcggtga cacatccgca atgggcgtgg gatgtgggcc
     7561 tgaacggtcg tccacatgat ttaggtaata tctcagctta tctcggcaaa ccgaccggac
     7621 tggaagatta catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc
     7681 ttgagtggat ccgcgatttc tgggatggcc cgatggtgat caaagggatc ctcgatccgg
     7741 aagatgcgcg cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg
     7801 gccgccagct ggacggtgta ctctcttccg cccgtgcact gcctgctatt gcagatgcgg
     7861 tgaaaggtga tatcgccatt ctggcggata gcggaattcg taacgggctt gatgtcgtgc
     7921 gtatgattgc gctcggtgcc gacaccgtac tgctgggtcg tgctttcctg tatgcgctgg
     7981 caacagcggg ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa gagatgaaag
     8041 tggcgatgac gctgactggc gcgaaatcga tcagcgaaat tacgcaagat tcgctggtgc
     8101 aggggctggg taaagagttg cctgcggcac tggctcccat ggcgaaaggg aatgcggcat
     8161 agtcgtttgc gccccctcac cctaaccctc tccctcaggg agctgtctct tgatcagatc
     8221 tcctgatcaa gagacagggg gagggccggg gtgaggggaa aaggccgcac tgctcaccaa
     8281 ttttctgcta tcctgccccc gcactaaggg ggcagtatgc taaacatcgt actttacgaa
     8341 ccagaaattc cgccaaatac tggcaacatc atccgtcttt gcgctaatac cggctttcgt
     8401 ctgcatatca tcgaaccgat gggatttgcc tgggacgata agcgcctgcg ccgcgcgggg
     8461 ctggactatc acgagtttac cgccgttacg cgtcatcatg actacagcgc gttcctcgaa
     8521 gcagaaaatc cccagcgcct gtttgccctc accacgaaag gtacgcctgc tcacagcgcc
     8581 gtaagctatc aggatggcga ttatctgatg ttcggcccgg aaacacgcgg cctgccagcg
     8641 agcattcttg atgccctgcc cgctgaacaa aaaattcgga ttccgatggt gccagacagc
     8701 cgcagcatga acctgtccaa tgcggtgtcg gtagtggtgt atgaagcctg gcggcagctg
     8761 ggatatccgg gagcggtgtt gagagattag ttacgccatg ccggatagat gattacatcg
     8821 catccggcac gatcacagga catcagatcc catccccata ctcaaatgta tggttaatac
     8881 cgttgaaatg ctggtccata tccattgatg gcttatcgct gtctggttta ccgacaatgc
     8941 gtgccggaac gccagcggcg gtggtatgcg gcggcaccgg ttgcagcacc acggaacctg
     9001 cgccaatctt cgcgccgcgc ccaacttcaa tattgccgag aatcttcgcg cccgcgccaa
     9061 tcatcacgcc ttcacgaatt ttcgggtgac ggtcgccacc agatttaccc gtaccgccaa
     9121 gcgtcacaga ttgcagaatc gatacgtcgt tttcaatcac cgccgtttca ccaacgacga
     9181 tgcctgtcgc gtggtcaagc atgataccgc gaccaatttt tgctgccggg tgaatatcga
     9241 cctggaacgt cacagaaacc tggttttgca gaaagattgc caatgcgcga cgcccctgat
     9301 tccacaacca gtgaccgatg cgataggcct gcaaggcatg aaaacccttc aggtataaca
     9361 acggggttga gtatttatcg actgccgggt cgcgggtacg caccgcctga atatcacagg
     9421 ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc ttccaccact tcacggatag
     9481 caatagcagg cataattggc gatgacagct tgttcgccag catgtagctc agtgcactgc
     9541 caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact ggccagcatt ggctcacagt
     9601 ccgccagcgt tctggcttcg gctttaatat tgttccagac aatttccagt tcttcacacg
     9661 acattgctta ctccacacga tgagataatg accggcccgt tctgcgcggg ccgggtcata
     9721 gcggtaacaa aggttccctg gggttagtgg ctgctgcgct cgtccttgcg tgcacgacct
     9781 aataacgtca atgctgcctc gcgcgcgttt tttccgcaat ataatacttg ataaatttcc
     9841 tcggttattg gcatttcaac gccgaagcga tgcgccagtt cgcggacttc tttcgtattg
     9901 cggtagcctt ccaccacctg accaatcttc tcctgcgcgc tttgtacatc catgccctga
     9961 ccgagcatca tgccaaaacg gcggttacgc gactggttgt cggtacaggt aagcaccaga
    10021 tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg cacccagcgc cgcgccaaga
    10081 cgcgacattt cagccaggcc acgggtgatt agcgccgtac gcgcattcgc gccaaaaccg
    10141 ataccgtcgg acatccccgc gccaatggca atgacgtttt tcaccgcgcc gccaagctgc
    10201 acgccaatga aatccgggtt actgtaaacg cggaaacttt taccgcagtg cagcagctgc
    10261 tgaagatcat cagcaaaagt ttggtcggtc gaggccagcg aaatggctgt cggtaaacct
    10321 gccgccagtt ctttcgcaaa cgttgggcca gagataaccg ccagcggaat ttgatcgcct
    10381 aacgcctcgc gggccacgtc ctgtaacaga cgcccggttt ccgcttccag ccctttggtc
    10441 gcccacacca gacgcgcatc aggacgcatc agcggtttaa tctggcgcag cacttcacca
    10501 aagacatggc tgggtacgac gacgagaata ttacggctgg ctgccagcgc agtggcgaga
    10561 tcgctttcaa gatggagtgt atcgggaaaa ggcacatcgg ggagaaacgc ggcgttacag
    10621 cggtcgcgtt caagcgttgc gatatgttca gggtcatggc cccagaggac aacctcgtgg
    10681 ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg agccggcacc gatcacagtc
    10741 attgaagcat tacgttggtt catcaggcat cctgatgttc ttcagtacct tcgccagcct
    10801 gctgctgcaa atagttcatg aacagcgcat cgaagttaac cggcgcaagg ttcagttgcg
    10861 ggaatgtacc gcgggatacc atgctggtga tgcactcacg agcatacggg aacagaatgt
    10921 tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc ttcgataccc gcgatggaga
    10981 aaataccgcc ctgctgaact tcacacagga acgcggtttc ttcgcccagg gaggccgtta
    11041 cggtaacacg cagtaccact tcgtaaacgt catctgccag ttgagtagat gccgtatcca
    11101 gatcaagttt aacttctggc tgccaatctt tctggaaaac gtgcggcgcg ttcggcgctt
    11161 cgaaagaaat atccttggta taaatacgtt ggatctggaa agtcatttcg gtgttgtttt
    11221 gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa atactgcgca atgtaatgcc
    11281 aacgttcaca cgttatttca gcaggggatc cagtccacca cgtgcatcca atgcatacaa
    11341 gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc tggggaaccg tggtgcgacc
    11401 gctgcgtttg atcatctctt cacgcttggc ggcgttgcca tcgatcggca gctcctggaa
    11461 actcacgccc ttgctgctca gcagtgcttt tgcacgatgg caatacgggc aggtttcttt
    11521 ggtatagatt tcaacattgg ccataaatta gctcctgttt ttttaccctg aaatttcatg
    11581 ttgcagggcg gcagcaaaat ggagtgtccc cagaagctta taacaataag tcgctggggg
    11641 cctaattgca gctaacggcc tgcatcatga aagacgacag gtaaattatt tgccgcgcac
    11701 caaaggcaag ttttcgccag cccagccagc gacgccttct ttcagcacga atacttgcgc
    11761 aaaaccagct ttcgtcagtg cgtttgcagg ctcctggcac tgcatgccag aaccgtctac
    11821 cacaataacc ggtttgtctt tgtgcttctc aagctcacca acattgttgg ctttgatttc
    11881 gctcggcaac agattaatag aacctgcgat atggccttta cggaagtcat cacgctgacg
    11941 taaatccaca accacagcgt cttctttgtt gatcagacgc gtagcttcac cacgagtaat
    12001 caccttcact ttcgaggtca ggctcttaaa cgtagtcaca agaaccgcca ccagtaacgc
    12061 gatccaggcg atactcagta tgggatgacg gccaacaaat tgcataattt cttgcatggg
    12121 gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa aggagtatac ctgcgcggtg
    12181 cggcaaatac agccagcgcg ttaactggaa tgcaattttg cggggcgcga cgaaaaaaaa
    12241 gcgtaaatct ggcatcggct tgccgcgagc ggtcgtatat tttgatcttc agaggctatt
    12301 ttatcgattc agctgtagta aaattacgca aattttgact cttgagtatg aggttgtcgc
    12361 aatgtcggtt tctaaaaaac ctatggtact ggtgattctg gatggctatg gctatcgcga
    12421 agaacagcag gataacgcca tttttagtgc taaaaccccg gtaatggatg cactgtgggc
    12481 caatcgtccg cataccctga tcgacgcttc cggtctggaa gtcggtctgc ctgaccgtca
    12541 gatgggtaac tccgaagtag gccacgttaa cctgggtgcc ggccgcatcg tgtatcagga
    12601 cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc tttgctaatc cggtgctgac
    12661 tggcgcggta gataaagcga aaaacgcagg taaagcagta cacattatgg gtctgctctc
    12721 cgcaggcggc gtacacagcc acgaagatca catcatggcg atggtagaac tggcagctga
    12781 acgcggcgca gaaaaaatct acctgcacgc attccttgac ggtcgcgaca ctccgccgcg
    12841 cagtgctgaa tcctcgctga aaaaatttga agagaaattt gccgcgctgg gtaaaggccg
    12901 cgtagcgtcc atcattggtc gttactacgc gatggaccgc gataaccgtt gggatcgcgt
    12961 tgaaaaagct tatgacctgc tgactctggc gcagggtgag ttccaggccg ataccgccgt
    13021 tgctggtttg caggctgctt atgctcgcga cgaaaacgac gagttcgtga aagcgaccgt
    13081 tattcgtgca gaaggtcagt cagatgcggc aatggaagac ggcgatgcgc tgattttcat
    13141 gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgct ttcgtgaacg ctgatttcga
    13201 tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg atgctgaccg aatacgccgc
    13261 tgacatcaaa actgcggttg cttacccacc agcttccctg gttaacacct tcggcgagtg
    13321 gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa accgaaaaat atgcccacgt
    13381 tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa ggcgaagatc gcattctgat
    13441 caactcaccg aaagtagcta cctacgatct gcaaccggaa atgagctccg cagagctgac
    13501 cgaaaaactg gttgcggcca tcaagagcgg caaatacgac accatcatct gtaactatcc
    13561 gaacggcgac atggtaggtc acaccggtgt gatggaagcg gcggttaaag cggttgaagc
    13621 actggatcac tgcgtggaag aagtcgcgaa agcggttgag tccgtgggtg gacaactgct
    13681 gatcaccgct gaccacggta acgctgagca gatgcgcgat ccggcaacgg gtcaggcaca
    13741 tacggcacac accaacctgc cagttccgct gatttacgtt ggcgataaga acgtgaaagc
    13801 ggttgaaggc ggcaaacttt ctgacatcgc gccgaccatg ttgtcgctga tgggtatgga
    13861 aatcccgcaa gagatgactg gtaagccgct gttcatcgtg gaataatccc tccccatgag
    13921 gggaaaggcg attaatacca tgacacgggc cgtgaaaccg cgcaggtttg caatcaggcc
    13981 catcatctac gccagcgttc ttagcgctgg cgtattgttg tgcgcctttt ccgcccacgc
    14041 ggatgagcgt gaccaactca aatctattca ggccgatatc gccgcgaaag agcgcgcggt
    14101 acgccaaaag caacaacaac gcgcaagcct gctcgcacaa ttgaaaaagc aggaagaagc
    14161 gatctctgaa gccacccgta agctgcgcga aacgcaaaac acgctcaatc aactcaataa
    14221 acagattgat gagatgaacg cgtcgattgc caaactggag cagcaaaaag ccgcccagga
    14281 gcgcagcctc gccgcacaac tggatgccgc attccgtcag ggcgagcata ccggtattca
    14341 gctgattctc agcggtgaag aaagccagcg tggacagcgt ttacaggctt atttcggcta
    14401 tctcaaccag gcgcgacaag aaaccattgc ccagttgaag caaacgcgtg aagaagtcgc
    14461 catgcagcgt gctgaactgg aagagaaaca gagcgagcaa caaacgctgt tatatgaaca
    14521 gcgcgcccaa caggcgaaac tgactcaggc gctgaacgag cgtaaaaaga cgctggcagg
    14581 gctggagtct tccatccagc aaggtcagca acagttgagc gagctgcgcg ccaacgaatc
    14641 ccgtctgcgt aacagcattg cccgtgcgga agccgcggcg aaagcgcgtg cagaacgaga
    14701 agcacgtgag gcccaggcgg ttcgcgaccg ccagaaagaa gcgacgcgta aaggcaccac
    14761 ctacaaaccg actgaaagcg aaaaatcgct gatgtcccgt accggtggtc tgggcgcgcc
    14821 gcgtggtcag gcattctggc cggttcgcgg gccgacgctg catcgctatg gcgaacagct
    14881 acagggtgaa ttacgctgga aagggatggt gattggtgcc tctgaaggta ctgaagttaa
    14941 agcgattgcc gacggtcggg tgattctggc tgactggctg caaggctacg gtctggtggt
    15001 ggtggttgag catggtaaag gcgacatgag tctttacggc tataatcaga gcgcactggt
    15061 gagcgttggt tcgcaggttc gcgcgggcca gccaattgca ctggtgggca gcagtggcgg
    15121 tcagggtcgg ccttcactct atttcgaaat tcgccgccag ggtcaggcgg tcaatccaca
    15181 gccgtggttg ggaagataag ttttgtttcc atttcgtcgt aacgttcttg catttgccgc
    15241 tctgttggcg ctctcctccc ccgtacttgc tggcaaactt gccatcgtca ttgatgattt
    15301 tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg ccttccgcta tctccgtcgc
    15361 tgtattaccc gattcaccgc acgccagaga gatggcgacc aaagcgcata acagcggtca
    15421 cgaagtgttg attcatctcc cgatggcacc gttgagtaaa cagccgctgg agaaaaatac
    15481 gctacgcccg gagatgagca gcgacgaaat tgagcgcatt attcgtagtg cggtcaataa
    15541 cgtgccctat gccgtgggga tcaacaacca catgggcagc aagatgacct ctaacctgtt
    15601 tggtatgcag aaagtgatgc aggcgctgga gcgttacaat ctttacttcc tcgacagcgt
    15661 aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggc actggcgtga aggtgatcaa
    15721 acggaaggta ttcctcgacg attcgcaaaa tgaagcggac atccgtgtgc aatttaatcg
    15781 cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc attgggcatc ctcacccttc
    15841 aacggtacgc gtgctacaac agatggttta taacctgccg ccagacatta cgctggtgaa
    15901 agccagcagc ttgctgaatg aaccgcaggt tgatacgtct acaccgccga aaaacactgt
    15961 gcccgacaca ccgcgtaatc cgttccgtgg cgtgaagctg tgcaaaccga agaaaccgat
    16021 agaacctgtt tatgctaatc gcttctttga agtattaagc gaaagcatca gccagagcac
    16081 gctgatcgtt tacttccagc atcagtggca aggctggggc aaacagcccg aagcggcgaa
    16141 acttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc gatggtgcga ccgcgttttg
    16201 tcgcgccatt gccataagcg gaaagaccac agtaacgcct gataacccac cttcacgctg
    16261 cgcacattgg taatcaggcg cttatacata ccggaagtaa atatctcggc aatcatccgt
    16321 tggcgggtaa gaatatccgg ctctttgcgc accgcatggc aaacacgcaa tgcttcgtaa
    16381 gttatttgct gatgaaattc cggataaatc gtaattttgt cggcataatt tcgatttaat
    16441 ttctccagca ggcgggtaat cttaatatag tgacgttgat aattaaggtt tttattccct
    16501 tgtctatgca accgactcac tgacgtatta tgcagataat atttatataa tgattgctcg
    16561 gtatatcgcg cacgcagcgc attaaacatg aattctgttg tccagacaat atcctgatga
    16621 tgtaatccgg caataaattt aatgttattt ttaacaataa catcgcggcg ataaaccccc
    16681 atccagacaa cgtgagtcca acggcgcgaa gaaagcccca tccgcagcca gtccgggccg
    16741 gttaatacac cggttgagcg aaggcgatcg ctggggatgg attgccaggt ttctcccgtt
    16801 tcacgaaaac accagtcagc gttgcactgc gccacgtcga ggtcgtcctc taacgccatg
    16861 gtcatcagcg tttcgtacat ggtgggatag acttcatcgt cagcatcgac aaaagcgaca
    16921 tatttgcccg ttgccacttc aatcccacga ttacgcgcca ccgatgcgcc agcattcgcc
    16981 tgatgcaaca aacgaacgtg cggatagttt tctgcgtaat gctttgctat ttcaacagaa
    17041 ttatccgttg aaccatcgtt aataataatg atttccagag cagtccaggt ttgcgtaatt
    17101 aaagattcca tacaagtgcg gaaatcatcg cccgcattat ataacggaat aataacacta
    17161 agtttattgg tgctgttcat cataaattac cgacgacctc tgaagttatc ctgacgtttt
    17221 acaggctttg tattaagaaa ctattaagcc tgtgagatat tatttttaca ggtgagagag
    17281 tcacttcagg gtagagagat aatgagagca gctatataag agatcagcac aattacgaac
    17341 tctttattac actgacagga ttttacatat ttaacagatt atcctcagat aaaagggctg
    17401 gaataccagc ccttgttcgt gttaatccca gctcagaata actttcccgg actggcccga
    17461 acgcatagcg tcaaagccct tctggaaatc atcgatagag aaccgatgag taatgatcgg
    17521 cgagagatcg aggccagact gaatcagcgc cgccatcttg taccaggttt caaacatctc
    17581 acgaccgtaa atgcctttaa tgaacaagcc cttaaagatc acttttgtcc agtctatcga
    17641 catgtcagaa ggtggaatac ccagcatcgc aatgcgaccg ccgtggttca tggtgtcgag
    17701 catggtacga aacgctggcg gcgcaccaga catttccaga ccgacatcaa agccttcggt
    17761 catgcctaac tccgccatca cgtcattgag attttctttg gcgacgttaa ccgcacgggt
    17821 gatacccatt ttacgcgcca gctcaaggcg gtattcatta acatccgtga tcaccacgtt
    17881 gcgtgcacca acgtgtttcg ccaccgccgc agccatgata cctatcggcc ctgcaccaga
    17941 gaccagcaca tcctcgccaa ccagatcgaa cgacagcgcg gtatgcacgg cgttaccgaa
    18001 gggatcaaaa atggaagcca ggtcgtcgga gatattgtct gggattttga aggcgttgaa
    18061 cgccgggatc accagatatt cggcgaagca gcccggacgg ttaacgccga cgccgatcgt
    18121 gttgcggcac agatgggtac gaccgccacg acagttgcga cagtgaccgc aagtgatatg
    18181 gccttcgcca gaaacgcgat cgccaatctt gaagcctttc acttcctgac caatacctac
    18241 cacttcaccg acatattcat ggcccacgac catcggcacc gggatggttt tttgcgacca
    18301 ctcatcccag ttatagatgt gaacgtcagt cccgcagatg gctgttttac ggattttaat
    18361 cagcagatcg ttatgcccga gttccggtac aggaacgtcg gtcatccaga tgccctcttc
    18421 cgctttcagt ttggataacg ctttcatctc acatcctcag gcaataacgc ccagttgttt
    18481 accaatacgc gtgaatgctt ctactgcacg cgtaatttgc tcaggggtat gcgccgcaga
    18541 catctgggta cgaatacgcg cctgaccttt cggaacgacc ggatagaaga aaccggtgac
    18601 gtaaatgccc tctttttgca gctcacgggc aaatttctgc gccactaccg catcaccaag
    18661 catgaccgga ataatggcgt gatcggctcc cgccagggta aagcccgccg ccgacatttg
    18721 ctcacggaac tgacgcgcgt tcgcccacag acggtcacgc agctcgctac ccgcttcgac
    18781 catctccagt actttgatgg atgcggcaac aatggccggt gccagcgagt tggagaacag
    18841 atacggacga gaacgctggc gcagccactc aaccacttct ttgcgcgccg cagtataacc
    18901 accagaagcc ccgcccagcg ctttaccaag cgtaccggtg ataatatcga cccggcccat
    18961 cacatcgcag tattcatggg aaccacgacc gttttcaccg acaaaaccga ccgcgtggga
    19021 gtcgtctacc atcaccaggg catcgtattt atctgccaga tcgcaaacgc ccttcagatt
    19081 ggcaatcacg ccgtccatcg agaacacacc atcggtggca atcagcacat gacgcgcacc
    19141 ggcttcacgc gcttctttca gacgtgcttc aagctcctgc atatcgttgt tggcatagcg
    19201 atagcgttta gctttacaca gacgcacacc atcaataata gacgcgtggt tcagtgcgtc
    19261 ggagataatg gcgtcttccg cgcccagcag cgtttcaaac aggccaccgt tagcatcaaa
    19321 gcaggaagag tagagaatcg catcttccat ccccaggaat gccgccagtt tttgttcaag
    19381 ctctttatgg ctgtcctgag tgccgcaaat aaaacgcacc gaagccatgc cgaaaccgtg
    19441 agaatccatt cccgcctttg ccgccgcaat cagatccgga tgattcgcca gcccgagata
    19501 gttattggca caaaagttaa tgacgtggct tccatcagcc acagtgatat ctgcttgctg
    19561 cgcagacgta ataatgcgct cttctttaaa caacccttcc gcccgtgcgg tttccagatc
    19621 gttggttaac tgctgataaa attctccacg cattgcgatt ctccagacag ggcaaattcc
    19681 agcacatatt acccaaactt ataggtcgcg acgagatgac gcgttaacac ttctgcaaaa
    19741 ttcaggataa ataacgtcca tcaatctcac ttttggcgaa tggctgaagc caacgccgtg
    19801 tagtgatatg atagaagtat tcgtgtctga gattgtctct gactccataa ttcgaaggtt
    19861 acagttatga tcatcgttac cggcggcgcg ggctttatcg gcagcaacat cgttaaggcc
    19921 ctgaatgata aaggcatcac cgatattctg gtggtggaca acctgaaaga cggcaccaag
    19981 tttgtgaacc tggtggatct gaatatcgca gactatatgg ataaggaaga cttcctgatc
    20041 cagattatgg ctggcgaaga gttcggcgat gtcgaagcga ttttccacga aggcgcatgc
    20101 tcttccacca ccgagtggga tggcaagtat atgatggata acaactatca atactccaaa
    20161 gagctgctgc actactgtct ggagcgcgaa atcccgttcc tgtatgcatc ttccgcggcc
    20221 acctacggcg gacgcacctc cgactttatt gaatcccgcg agtacgaaaa accgttgaac
    20281 gtctacggtt actcaaaatt cctgtttgat gaatatgttc gtcaaatcct gcctgaagcg
    20341 aactcgcaga ttgttggctt ccgttatttc aacgtttatg gaccgcgtga aggccataaa
    20401 ggcagcatgg cgagcgtcgc tttccatctc aacacccagc ttaacaacgg tgaatcaccg
    20461 aagctgtttg aaggtagcga gaacttcaaa cgcgacttcg tctatgtggg cgacgtggca
    20521 gatgtaaacc tgtggttcct ggaaaatggc gtttccggca tcttcaatct cggtactggt
    20581 cgtgcggaat ccttccaggc tgtagctgat gctacgctgg cttatcacaa gaaaggccag
    20641 atcgaataca ttccgttccc ggataaactg aaaggccgct atcaggcgtt cactcaggca
    20701 gatctgacaa atctgcgcgc ggcgggttac gacaaaccgt tcaaaaccgt tgctgaaggt
    20761 gtaacggaat acatggcctg gctgaatcgc gacgcataag agctctgcat gaaaatactg
    20821 gtgatcggcc cgtcttgggt tggcgacatg atgatgtcgc aaagtctcta tcgcacgctc
    20881 caggcgcgct atctccaggc gataatcgat gtgatggcac cggcatggtg ccgtccatta
    20941 ttatcgcgga tgccggaagt taacgaagct attcctatgc ctctcggtca cggagcgctg
    21001 gaaatcggcg aacgccgcaa actgggtcat agcctgcgtg aaaagcgcta cgaccgcgcc
    21061 tacgtcttac ccaactcctt caaatctgca ttagtgcctt tcttcgcggg tattcctcat
    21121 cgcaccggct ggcgcggcga gatgcgctac ggtttactca acgatgtacg cgtgctcgat
    21181 aaagaagcct ggccgctaat ggtggaacgc tatatagcgc tggcctatga caaaggcatt
    21241 atgcgcacag cacaagatct gccgcagcca ttgttatggc cgcagttgca ggtgagcgaa
    21301 ggtgaaaaat catatacctg taatcaattt tcgctttcat cagaacgtcc gatgattggt
    21361 ttttgcccgg gtgcggagtt tggtccggca aaacgctggc cacactacca ctatgcggag
    21421 ctggcaaagc agctgattga tgaaggttat caggtggttc tgtttggctc ggcgaaagat
    21481 catgaagcgg gcaatgagat tcttgccgct ttgaataccg agcagcaggc atggtgtcgg
    21541 aacctggcgg gggaaacaca gcttgatcaa gcggttatcc tgattgcagc ctgtaaagcc
    21601 attgtcacta acgattctgg cctgatgcat gttgcggcgg cgctcaatcg tccgctggtt
    21661 gccctgtatg gtccgagtag cccggacttc acaccgccgc tatcccataa agcgcgcgtg
    21721 atccgtttga ttaccggcta tcacaaagtg cgtaaaggtg acgctgcgga gggttatcac
    21781 cagagcttaa tcgacattac tccccagcgc gtactggaag aactcaacgc gctattgtta
    21841 caagaggaag cctgacggat gcgggttttg atcgttaaaa catcgtcgat gggcgatgtt
    21901 ctccatacgt tgcccgcact cactgatgcc cagcaggcaa tcccagggat taagtttgac
    21961 tgggtggtgg aagaagggtt cgcacagatt ccttcctggc acgctgccgt tgagcgagtt
    22021 attcctgtgg caatacgtcg ctggcgtaaa gcctggttct cggcccccat aaaagcggaa
    22081 cgcaaagcgt ttcgtgaagc gctacaagca gagaactatg acgcagttat cgacgctcag
    22141 gggctggtaa aaagcgcggc gctggtgacg cgtctggcgc atggcgtaaa gcatggcatg
    22201 gactggcaaa ccgctcgcga acctttagcc agtctgtttt acaatcgtaa gcatcatatt
    22261 gcaaaacagc agcacgccgt agaacgcacc cgcgaactgt ttgccaaaag tctgggctat
    22321 agcaaaccgc aaacccaggg cgattatgct attgcacagc atttcctgac gaacctgcct
    22381 acagatgctg gcgaatatgc cgtatttctt catgcaacga cccgtgatga taaacactgg
    22441 ccggaagaac actggcgaga attgattggt ttactggctg attcaggaat acggattaaa
    22501 cttccgtggg gcgcgccgca tgaggaagaa cgggcgaaac gactggcgga aggatttgct
    22561 tatgttgaag tattgccgaa gatgagtctg gaaggcgttg cccgcgtgct ggccggggct
    22621 aagtttgtgg tttcagtgga tacggggtta agccatttaa cggcggcact ggatagaccc
    22681 aatatcacgg tttatggacc taccgaccct ggattaattg gcgggtatgg gaagaatcag
    22741 atggtttgta gggctccgct aaagagttta aataatttag aagcagcggc tgtttataaa
    22801 gaaattacat tga
//
LOCUS       DAZUCE010000065        22711 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZUCE010000065 DAZUCE010000000
VERSION     DAZUCE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.065"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            199..618
                     /locus_tag="ACHY03_002082"
     CDS             199..618
                     /locus_tag="ACHY03_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFS3905313.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            618..1886
                     /gene="umuC"
                     /locus_tag="ACHY03_002083"
     CDS             618..1886
                     /gene="umuC"
                     /locus_tag="ACHY03_002083"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFS3905314.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVVQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1932..2462)
                     /gene="dsbB"
                     /locus_tag="ACHY03_002084"
     CDS             complement(1932..2462)
                     /gene="dsbB"
                     /locus_tag="ACHY03_002084"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFS3905315.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2608..4149)
                     /gene="nhaB"
                     /locus_tag="ACHY03_002085"
     CDS             complement(2608..4149)
                     /gene="nhaB"
                     /locus_tag="ACHY03_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFS3905316.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4371..5090
                     /gene="fadR"
                     /locus_tag="ACHY03_002086"
     CDS             4371..5090
                     /gene="fadR"
                     /locus_tag="ACHY03_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFS3905317.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5142..6674)
                     /locus_tag="ACHY03_002087"
     CDS             complement(5142..6674)
                     /locus_tag="ACHY03_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFS3905318.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7003..8301
                     /gene="dadA"
                     /locus_tag="ACHY03_002088"
     CDS             7003..8301
                     /gene="dadA"
                     /locus_tag="ACHY03_002088"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFS3905319.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRARFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8311..9381
                     /gene="dadX"
                     /locus_tag="ACHY03_002089"
     CDS             8311..9381
                     /gene="dadX"
                     /locus_tag="ACHY03_002089"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFS3905320.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGARYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9550..10977)
                     /locus_tag="ACHY03_002090"
     CDS             complement(9550..10977)
                     /locus_tag="ACHY03_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905321.1"
                     /translation="MASFKDICPFAPAIPPSSVNTGVRRPPRRLFLSVELKNQILQNM
                     KYLSPQKFSWGDAPWQIIDLSIAGKVNIQVDNNTIITLGTPLNPQHNEFMMVAEWCQW
                     AMERDGLQENLQKNLYEILEENQQNKQSEIPQEDLKESLEEIKVNILEENLPASRIEN
                     RAEALRRMKECLITRRSMLNLSNLGLTSLPENLPPHLIEFYCSKNVLTALPKVMPKWL
                     LVLDCTDNVLILLPKVQPSKLMVLNCYDNCIIWLPELSTNLRVINCSANFLQFLPPSM
                     PQYLYKLSCAGNNIHSIPDEMLENLTRLKVFDCSSNDLISSPRLPPKLIIYYCGENKF
                     KTVQVPQPQSLKVFDCNGNPWDKDNLPTLLKAVEGLKKQQGLKDLLGFLHKEGQMKLE
                     KLEDLDDLMDLELLDDPVLLERVKVQEDLELLNQQLGLLSLERQQDSQPVNQQSEHEP
                     EPASRVKRDLSEVDSESTMKRKRFM"
     gene            11259..11495
                     /locus_tag="ACHY03_002091"
     CDS             11259..11495
                     /locus_tag="ACHY03_002091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905322.1"
                     /translation="MKRVLRGLRQRGRLRGGICSGDVRLGPLMVDGDTGNAGLVILHL
                     LQSEEAITLTFLSRCFEFIGQSLQWFVIHNLTFF"
     gene            complement(11598..13334)
                     /locus_tag="ACHY03_002092"
     CDS             complement(11598..13334)
                     /locus_tag="ACHY03_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFS3905323.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(13429..14343)
                     /gene="ldcA"
                     /locus_tag="ACHY03_002093"
     CDS             complement(13429..14343)
                     /gene="ldcA"
                     /locus_tag="ACHY03_002093"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFS3905324.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAMLNNTREGTQLTISGHPVLKM"
     gene            14443..15054
                     /gene="emtA"
                     /locus_tag="ACHY03_002094"
     CDS             14443..15054
                     /gene="emtA"
                     /locus_tag="ACHY03_002094"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFS3905325.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(15056..15790)
                     /locus_tag="ACHY03_002095"
     CDS             complement(15056..15790)
                     /locus_tag="ACHY03_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFS3905326.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            15991..16245
                     /locus_tag="ACHY03_002096"
     CDS             15991..16245
                     /locus_tag="ACHY03_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS3905327.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(16295..18265)
                     /locus_tag="ACHY03_002097"
     CDS             complement(16295..18265)
                     /locus_tag="ACHY03_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFS3905328.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGNHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(18291..19145)
                     /gene="modD"
                     /locus_tag="ACHY03_002098"
     CDS             complement(18291..19145)
                     /gene="modD"
                     /locus_tag="ACHY03_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFS3905329.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            complement(19142..19954)
                     /locus_tag="ACHY03_002099"
     CDS             complement(19142..19954)
                     /locus_tag="ACHY03_002099"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS3905330.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLISKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            complement(19964..20722)
                     /locus_tag="ACHY03_002100"
     CDS             complement(19964..20722)
                     /locus_tag="ACHY03_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3905331.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSVLGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     AATIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            complement(20719..21699)
                     /locus_tag="ACHY03_002101"
     CDS             complement(20719..21699)
                     /locus_tag="ACHY03_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFS3905332.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(21699..>22711)
                     /locus_tag="ACHY03_002102"
     CDS             complement(21699..>22711)
                     /locus_tag="ACHY03_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFS3905333.1"
                     /translation="RRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITRIVSA
                     GAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSLEGLM
                     ALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLGEEHR
                     AAEQAYLASRFVGEAQAFATSPAANLRFYAARGPRGLETGLKGSLHTEAAELLGLHNV
                     AQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRILFLSG
                     LPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADYQKLV
                     AS"
BASE COUNT         5616 a         5715 c         5969 g         5411 t
ORIGIN      
        1 ttctattttt tagccgggtg atatttttca tttctgctgg atgagcgtcg tcgccagaag
       61 gccgcgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg acaaatattg
      121 atagcctgaa tcagtattga tctgctggca agaacagact actgtatata aaaacagtat
      181 aacttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga aattgtgact
      241 tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggccgc agattacgtt
      301 gaacagcgca tcgatctgaa tcaactgttg atccagcatc ccagcgcgac ttacttcgtc
      361 aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt actgattgtc
      421 gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga cggcgagttt
      481 acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa cagcgcgtac
      541 tcgcccatta ccatcagtag tgaagatacg ctggatgtct ttggtgtggt gatccacgtc
      601 gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat gccagctgtg
      661 agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg aataatgacg
      721 gttgcgttat cgcccgaaac gctgaggcaa aggcgcttgg cgttaaaatg ggcgatccct
      781 ggttcaaaca aaaagatctg tttcgtcgct gtggcgtggt ttgctttagc agcaattatg
      841 agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta tcgccccgcg
      901 tcgagattta cagtattgat gaggcattct gcgatctgac aggtgtgcgt aattgtcgcg
      961 atctgactga ctttggcaga gaaattcgcg caacggtgct acaacgtacc catcttactg
     1021 ttggtgtggg gatcgcccag accaaaacgc tggctaagct tgccaatcat gcggcaaaaa
     1081 aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc cagcgtaaat
     1141 taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc agcaaaaaac
     1201 tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc cggtttatcc
     1261 gtaaacattt taatgtcgtg ctcgaaagaa cggtgcgtga actgcgcggc gaaccctgtt
     1321 tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc tcgtttggtg
     1381 aacgcatcac ggattatacg tcgatgcggc aggccatttg tagttacgct gcccgggcgg
     1441 cggaaaaact tcgcagcgag catcaatatt gtcggtttat ctccacgttt attaagacgt
     1501 caccatttgc gctcaatgaa ccttattacg gcaatagcgc gtcggtaaaa ctgctgacgc
     1561 ccactcagga cagcagggat atcattaacg ccgctacgcg atctctggat gccatctggc
     1621 aagcgggcca tcgttaccaa aaagcgggcg tgatgctggg ggatttcttc agtcagggag
     1681 tcgtgcagct caatttattc gatgacaacg ctccgcgccc cgggagtgag caattgatga
     1741 cggtaatgga tacactgaat gctaaagagg gcagaggaac actctatttt gccgggcagg
     1801 ggatccagca acaatggcag atgaagcgag ccatgctttc accacgttat acaacgcgaa
     1861 gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgct cccgcaggag
     1921 cgccgaatgg attagcgacc gaacagatca cgttttttcg ctttaaacgg ctgggaaatc
     1981 accaccagca ctgcgacaat caggtaagcg ataaaaatac cgagcagcca ctgcggcatt
     2041 tccagaccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac aaacacttgc
     2101 ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc acaggtggca
     2161 aatggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac accgcggaac
     2221 gcactataca accagataac catcgctaca taacgcagtg gcgtttttgg ggcgatcgcg
     2281 ccaatcagcg cagcacccag aacgccgaat aatgcgcagc gttcataaat acagagcacg
     2341 caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag tgccagagca
     2401 gtaaacgcca tcaacagcca cgcgccccgg ccttgtgaac attggttcaa aaatcgcaac
     2461 ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca ttcgctgcgc
     2521 caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa aggcaaaccg
     2581 ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca tttgcataaa
     2641 ccattcggtt acaggggcaa gcgtaaactc gacgcagagc agtccgacga gtgtcaggac
     2701 gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa tcaatggcgc
     2761 gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag cgacagaggg
     2821 cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca acgtgatagc
     2881 gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga agacgttatc
     2941 cgaaatggat gacagcagtc cgttgaaaat atagaacaac gacagctgag catgttccga
     3001 tgcctgcaac acaaactgaa taatcggcga aaacagttgt tggtcgataa tcaccgcgac
     3061 taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga aggctttacc
     3121 tatagcatgt tcatcggtga caccggtcaa tgatgttgcc agaataatga ctgacaaacc
     3181 aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga cgccaataat
     3241 cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc tttgatcgtc
     3301 aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc caaaccaacg
     3361 cagcttctct acaagcaggc aggttaacag gccacaaatc agaaccggaa cggtcaccgg
     3421 cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga tgatcaggtt
     3481 ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac cggcatgcat
     3541 catcaggcta cgcagaaagc cacggaactg ttccagaacc actttgtaat gcttgtcgat
     3601 atgactatcg tcttgcaggt cggtatcttc ggtacgggaa gaggctacgc gatgataaat
     3661 accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg catcgaggaa
     3721 cgcggagagg aacgctgccg ccacgcaaaa agagagcgac agcagcattt tggagcgaat
     3781 gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat agatacccgc
     3841 caccataaac atcaataaca gcaagacttc aagatttgcc gccacctctt cacggacgtg
     3901 ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac cggggagtag
     3961 cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca gcaaccagcc
     4021 agcgacgaaa gggctgatga ggaaaattaa cggatttacg attaagaaaa tgatgagggc
     4081 gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc ggccccagga
     4141 gatctccatg atggtttccc ttaccttaca aataatcaat gatgttttta tgtttaaacg
     4201 caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg aaagagatgc
     4261 ggaaaatgaa gccttgatcc cttttttctt ctttttgtct gctatcagcg tagttagccc
     4321 tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact atggtcatta
     4381 aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc tggaataacc
     4441 gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta attggcgtaa
     4501 cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg ttgaccattc
     4561 aacatggcaa gccgacgaag gtgaataatt tctgggaaac ctctggttta aatatccttg
     4621 aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat ttgctgtcgg
     4681 tgcgtaccaa tatttcaact atttttattc gcaccgcgtt tcgtcagcat cccgataaag
     4741 cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc tttgccgagc
     4801 tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt tacggtctga
     4861 tccttaacgg gatgaaagga ctgtatacgc gcattggtcg tcactatttc gccaatccgg
     4921 aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc agtgaaggcg
     4981 cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc gagatttggc
     5041 accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa tcccttccgt
     5101 ttaaaaagca aacccctcaa acgaggggtt ttttgttgtt tttacagatt tcccattctt
     5161 ggcgggcaac gttccagcaa ctcgacgctg ccgtcttcgt tttgctgttc cagcatcaca
     5221 tcaaatcccc acaggcgatg cacatgcttc agtacttctt tgcgcccccg atccagcggt
     5281 gcgcgattat gtggaatgta acgcagcgtc agcgaacggt cgccgcgcaa atccacgttc
     5341 cagatctgaa tattcggctc cagattactt aagttatatt gcgacgatag ccggttacgg
     5401 atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt atgccgatcg
     5461 tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggcgacag gaactggctg
     5521 ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag ccagtcggaa
     5581 ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca aatccgttta
     5641 atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta ccacgggctg
     5701 ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag cataaaacgt
     5761 tccgttactt tcccttcatc atacagatgg ttaaggatgg tgtagtgcca gaaggtggcc
     5821 cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct caccttacgc
     5881 acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt ctccataaaa
     5941 tagagcaggt tttcttgtgg ttcggacgga taacggcgcg cttcagcaac cgttttctct
     6001 tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgcaa atactcttcg
     6061 cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgtgggcg tttgtaccgg
     6121 tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac ttcatcaacg
     6181 ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata atcgacaatc
     6241 gaactggcgt cggtccagct acggaataag taattgtttt tgaagaaaga gttatgtcca
     6301 tagcaggcat gagccatcac cagcgcttgc atggtaatgg tgttctcttc catcaggtaa
     6361 gcgatacacg ggttagagtt aatgacgatt tcataggcca gtccttgctg accgtgctta
     6421 tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata gttaattggc
     6481 atgccgacgc tggagtaggc atccatcatc tgttctgagg ttatcacttc aatctggtgc
     6541 gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag ataaacatcc
     6601 agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt attcatagaa
     6661 tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg agcacctcat
     6721 ttcaagcata gaacacctgt taaaaaccgc gtcgccggag atttttttct ttgcgatttc
     6781 ttattatcag agtgccacta atccgcttct gaacggaatt ttatgctgga taaaaagggc
     6841 gttcagcagg agatactaaa gacgccatat tgccgcagag tcagggagat gtgagccagc
     6901 tcaccataaa aaagccgtat gttgaataat attttcaact gagttatcaa gatgtaatta
     6961 gattattatt cttttactgt atctaccgtt atcggagtgg ctatgcgagt tgtcatactg
     7021 ggaagtggtg tggtaggcgt tgccagcgcc tggtacttaa atcaggcagg acatgaggtc
     7081 accgtcattg atcgggagcc gggggccgcc ctggaaacca gtgccgcgaa tgccgggcaa
     7141 atctcccccg gatatgctgc accgtgggcg gcaccaggtg tgcctttaaa agcgattaaa
     7201 tggatgttcc agcgccatgc gccgctggcg gttcgtctcg acggtacaca attccagttg
     7261 aaatggatgt ggcaaatgtt acgtaactgc gacaccagcc actacatgga aaacaaaggg
     7321 cggatggtgc gtctggcgga atacagccgt gattgcctga aagcattgcg cgccgaaacc
     7381 aatattcagt atgaagggcg tcagggtggg acgctgcaac tgttccgtac cgaacaacag
     7441 tatgaaaatg cgacccgcga tatcgccgtg ctggaagatg ccggcgtacc gtatcagctg
     7501 ctggaatcca gccgcctggc ggaagtggag cccgcgctgg cagaagtggc gcacaaactg
     7561 acgggcggcc tgcagttacc caatgatgaa accggagact gtcagctatt tacccagaat
     7621 ctggcgcgga tggcggagca ggcgggggtt aaattccgct ttaatacgcc cgttgaccaa
     7681 ctgctttgcg acggcgagca aatctacggc gtgaagtgtg gcgatgaagt gattaaggcc
     7741 gatgcgtatg tgatggcgtt tggttcttac tcgacggcga tgctcaaagg cattgttgat
     7801 attccggtgt atccgctgaa aggctactcg ctgaccattc caattgcgca ggaagatggt
     7861 gcgccggtat ccaccattct tgatgaaacc tacaaaatcg ccattacccg tttcgataac
     7921 cgcattcgtg ttggcggaat ggcggaaatt gttggtttta ataccgagct gttgcaaccg
     7981 cgtcgtgaaa cgctggagat ggtggttcgc gatctctatc cacgcggcgg tcatgtcgag
     8041 caggcgactt tctggactgg tctgcgcccg atgacgccag acggcacgcc ggttgtcggg
     8101 cgtgcacgct ttaaaaatct gtggctgaat accggtcacg gcacgctcgg ctggacgatg
     8161 gcttgcggtt ccggtcagtt gttaagcgat ctgctctctg gtcgcacgcc agcgatccca
     8221 tatgaggatc taagcgtagc gcgctacagc cgtggattta cgccatcacg tccgggccat
     8281 ttacatggcg cacacagcta aggaaacgag atgacccgtc cgatacaggc cagcctcgat
     8341 ctgcaggcat taaaacagaa tctgtccatt gtccgccagg ccgcgccgca cgcgcgcgtc
     8401 tggtcggtgg taaaagcgaa cgcttacggg cacggtattg agcgtatctg gagcgcgctc
     8461 ggggctaccg atggctttgc attacttaac ctggaagagg caataacgtt acgtgagcgc
     8521 ggctggaaag gaccgatcct gatgctggaa ggatttttcc atgctcagga tctggagatt
     8581 tatgaccagc accgcctgac cacctgtgta cacagcaact ggcagctcaa agcactgcaa
     8641 aatgcgcggc taaaagcacc gttggatatt tatcttaaag tgaacagtgg gatgaatcgg
     8701 ttgggcttcc agcccgatcg cgtgcttacc gtctggcagc agttgcgggc gatggcgaat
     8761 gttggcgaaa tgaccctgat gtcgcatttt gccgaggcgg aacatcctga tggaatttcc
     8821 agcgcgatgg cgcgtattga gcaggcggcg gaagggctgg agtgtcggcg ttcgttgtcc
     8881 aattcggcgg cgactctgtg gcacccggaa gcgcattttg actgggttcg gcctggcatt
     8941 attttgtatg gcgcttcgcc gtccggtcag tggcgtgata tcgccaatac cggattacgt
     9001 ccggtgatga cgctaagcag tgagattatt ggtgtccaga cgctaaaagc gggcgagcgt
     9061 gtgggctacg gcgctcgtta tactgcgcgc gatgagcagc gaatcggcat tgtcgccgca
     9121 ggatacgccg acggttatcc gcgccatgcg cctaccggta cccctgtttt agtggacggc
     9181 gtgcgcacca tgacggtggg gacagtctcg atggatatgc tggcggtcga tttaacgcct
     9241 tgtccgcagg cgggtattgg tacgccagtt gagctgtggg gcaaggagat caaaattgat
     9301 gatgtcgccg ccgctgccgg aacggtgggc tatgagttga tgtgcgcgct ggcgttacgc
     9361 gtcccggttg tgacggtgta acttgttgta agccggatcg gaggcaacgt cttctggttg
     9421 caaaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga cagagccatt
     9481 gcccatgata gtgcccatta aaatgatgga cactatttcc ccggaacctg aactcaccgc
     9541 acaggcgttc tacataaaac gcttacgctt cattgttgac tcggaatcga cttcagataa
     9601 atcacgcttc acccttgatg caggttcggg ttcatgttca gattgttgat taacaggctg
     9661 cgagtcctgt tgtctttcca gactcaacag gcccaactgc tgattcagga gttccaggtc
     9721 ctcctgtacc ttcacgcgct ccaggagtac cgggtcatcc aggagctcca gatccataag
     9781 gtcatccaga tcctccagct tctccagctt catctggccc tccttgtgca aaaagcccaa
     9841 aaggtccttc aggccctgct gttttttcag gccctcgacg gctttgagca gcgtcggtaa
     9901 attgtctttg tcccacgggt taccattaca gtcaaacacc ttcaggctct ggggctgcgg
     9961 cacctgtacc gttttaaact tgttttctcc acagtagtaa attattagtt tgggcggcag
    10021 ccgtggtgaa gagatcaaat cgttactaga acagtcaaat actttcaggc gagtcaagtt
    10081 ctccagcatc tcatcaggta ttgagtgaat attgttgcca gcacaggaca gtttatacag
    10141 gtactggggc atcgacggcg gtaaaaattg caagaagttt gcagaacagt taatcactct
    10201 caggttagtc gacagctcag gcagccagat aatacagttg tcatagcaat tcagtaccat
    10261 cagttttgag ggctgcacct tcggtaacaa gatcaagacg ttatctgtac agtcaagcac
    10321 cagcagccac tttggcatca ccttcggtaa cgcggtcaac acgtttttac tgcaataaaa
    10381 ttcaatcaga tgtggaggca aattttcagg gagtgaagtc aatccaaggt tgctaagatt
    10441 gagcatactc cgtcttgtaa tgagacattc cttcattctg cgcaatgctt ctgcccggtt
    10501 ttcaatccgg cttgctggca ggttttcttc caggatattt accttgattt cttccagact
    10561 ttctttcagg tcttcttggg gaatttctga ctgtttgttt tgctggtttt cttccaggat
    10621 ttcgtacagg tttttttgca ggttttcttg caaaccatcc cgctccatag cccattggca
    10681 ccattcggcg accatcatga actcgttatg ttgcggattc aggggagtcc ccaacgtgat
    10741 gatggtattg ttgtccacct gtatgttcac tttgcccgcg atactgaggt caatgatctg
    10801 ccagggggca tcaccccagc tgaatttttg cgggctgaga tacttcatgt tctgcaaaat
    10861 ttgattcttg agttccaccg acaggaagag ccgccttggc gggcgtcgta cgcctgtgtt
    10921 aacggaagat gggggaatag cgggggcgaa tgggcagatg tctttaaagc tagccataat
    10981 gctacgtatc cttgaaataa atatcctctg gcagcgccgg agacgtgaat gtggtcatcg
    11041 caaaggagcc acaggacgtc atttgcgctg atataactac tcatcgttga ctttttccct
    11101 ggtcagaaat gccgtgtcgg gtctggtaca tgctgtatta cggcccaggt agctctgacg
    11161 tattaaagaa aatacgagag cgaacgaccc ggaaataatc gttattaagc aaaggaataa
    11221 ctataagaaa tcgctcatgc atccggtgct ataggcggat gaaacgcgta ttgcgtgggc
    11281 tacgtcagcg cggcaggttg cgtgggggaa tatgttcagg cgatgtcagg ctggggcccc
    11341 tgatggttga cggggatacc ggtaatgcgg ggctggttat tcttcacctg ttgcagtcgg
    11401 aagaagccat tacgctgact ttcttgagcc ggtgttttga gtttattggc cagagcctgc
    11461 aatggtttgt catccataat ttaaccttct tctgatgttg gaatgtgcat atcaaaatca
    11521 ggaaatgaca agtttatata caggctcagg ctgcgttaag caaagcatcg taaccggcga
    11581 tagatgcttc attaaattta agattcagct tcctcttcag ccactcgaac gccaatcttc
    11641 aacacttcat tatcttcttt ctcggccacc gtccagatca ttccggcaaa ctctacctgg
    11701 tcaccgacaa ccggtgccgc gcctaacaac tgctggacaa tttcacccag cgtttgctgt
    11761 ttatcacgat attctcgccc gtcttccagg ccatatatca gcgcaacatc agcatatttg
    11821 gcgctggctt cgagaatgaa gtcaccaaag aagcgttgat ccagcgcgac cggtggcgac
    11881 tggctgaaca gtttaccgag cgccgggaga tcgcgttccc gaccaattac acacaataca
    11941 tcgccttcgc gcagtcgggt gctgccggta ggatgaagca actggttatc acgaaacagg
    12001 gctgcaatac gcgtctcttt tggcatatgc aaatcgcgca gtgccgcacc tacacaccat
    12061 ttatcagcac tcaattgata aacaaactgt tcccacggat tttccggatg aatatccagg
    12121 ccaacgcgtg acaccggacg tccgacgggc ggaaccacca ctttggcctt tttagccgcc
    12181 cacgagagtg atgttccctg caagagcagt gaaaccagaa ccacaaagaa ggcgacatta
    12241 aagaacagac gtgcattctc cagccccgcc atcatcggga acacggccag aataatcggc
    12301 accgcgccgc gtaatcccac ccagctgata aacacgcgct cacgcagatt aaaaccacgg
    12361 aacggtagca atccggcaaa tactgaaaga ggacgggcga agaaaatcat ccatgcggac
    12421 aaaatgagcg ccggaatggc aataggcagc agatcgcttg ggttaaccaa cagccccagc
    12481 accaggaaca tggcgatttg cgccagccag gcgaggccgt cgaaattttg caggatgccg
    12541 tagcggttgc gaatcggccg attacccagc agaaaaccgc acagataaac agccagaata
    12601 ccgctacctt ccagcgccgt ggttaaagca aaaattaaaa taccgccgct taatgccagc
    12661 aatggatata aaccggcggg cagggcgatg cgattaatca tttgcagcag taaataacca
    12721 ccgccaagac cgatgacaat cccgaggcca aattgctgca gaatatcgac cacgaacatc
    12781 caactgacac tgctttcatg ttgctggatc atcgcaatca gggtaatcgt cagaaagacc
    12841 gccattggat cattactgcc ggattcaatt tccagcgttg agccaacacg ttcgttaagc
    12901 cccttaccac ccagcagaga aaagaccgct gcagcatcgg tggagccgac gatagcgccg
    12961 attaataagc cttcaatcaa atcaagatta aacagccacg ccgccatcat gccggttaaa
    13021 ccagaggtga taagtacgcc cagcgtcgcc agcgacagcg ccggtcctaa cgccacacga
    13081 aaggaactgg cctgagtgcg catcccgccg tcgagcaaaa taatcgccag cgccaggtta
    13141 cttaccatgt aggcgaaggg gtaattatca aacgggatac cgccgacgcc atcgactccc
    13201 gccagcatgc cgatcgccag aaaaataacc aaaataggaa tgccaagacg ggaagaaaat
    13261 gaactaagta atatactgct ggtgacaagg atggatccta agatgaaaag gctaattatt
    13321 gttgtggcat ccaacggtcg gttactcctg attacgctgt ctcttatata aaccctacca
    13381 tattagcggc agagacagcg ttttacttag ccctgaagcg tgattttttt acattttaag
    13441 aacaggatga ccgctaatcg tcaactgagt gccttcccgg gtgttattca gcatggcatg
    13501 tgcgcccaac ggcagcgtaa ccgtgcgttg ttcatgacca aaatcgaggc cggtaatgag
    13561 tggaatcgac aggcgggaac gcaaaaacgc gtacactgac tccaggttgt aacctgcgtc
    13621 ataatcatta ggcgtactgc cgctaaagct accgagaata atcgccttct gacgtggcaa
    13681 aattccagca tgatagagct gcaacagcat acgttcgacg cggaaagggt gctcgttaat
    13741 atcttccagc accagaatac cgttctcaat ttttggcatc cacggtgtac caatcagtga
    13801 aatcagcatc gcaagattgc ctccccacaa cgtgccttcg gtctgacatg tcgggccttc
    13861 cccttgccat tcaatggtga aggtttcatt gcgtaacgcc agccagaaat ggtgctcggt
    13921 aaaggcgttt agttcatccg cgccaaagtt tgctaccagc atggggccgc taaaggtgat
    13981 gacattgcct tgcgccagaa gaccgcactg aatggcggta aaatcgctat gcccgcatat
    14041 gagcaacggg tcatattgtt ggcgagccac cagtgcctgc cagtcaatat ctgccagtaa
    14101 acgactggca ccgtaaccgc cgcgtacagc cagcacgatg gtgttggggg tggtcagtct
    14161 ggcaagggaa ttgagatctt ccagacgctc cgtttccgta cccgcaaaac gctcacaacg
    14221 acgggcaata acctcgacgt tattgacttg atgccccgcg tcggtaaggc gctggatacc
    14281 acgcagcgcg gcgtgctgtt taatgcagta acccgatggg gcaattaagt gaaacagaga
    14341 catggcaatt ccttgctgac aacagaaacg aaatgtatat catgccgctt aggtgtgccg
    14401 ttgtcacctc aacggcgatt ccaggctata aggatagaag aagtgaaatt gagatggttt
    14461 gcctttttga ttgtgttatt agcgggttgt tcatcaaagc atgactacac gaacccgccg
    14521 tggaacgcga aagttccggt gcaacgtgcg atgcagtgga tgccaataag ccagaaagcc
    14581 ggtgcagcct ggggcgtcga tccacaattg atcacggcga ttatcgctat cgaatcgggt
    14641 ggtaatccga acgcggtgag taaatcgaat gccattggtt tgatgcagtt aaaagcttca
    14701 acctccggac gtgatgttta tcggcgtatg ggctggagtg gtgagccgac gaccagcgag
    14761 ctgaaaaatc cggagcgtaa tatttctatg ggggcggctt acctgaatat tctggaaacc
    14821 ggcccgctgg caggcattga agatccgaag gtactgcaat atgcgctggt ggtgtcatat
    14881 gctaacgggg caggtgcgct gctacggact ttctcgtcag atcggaaaaa ggcaatcagc
    14941 aaaatcaacg atctggatgc tgacgagttc ctcgatcacg tagcgcgaaa tcatcctgcg
    15001 ccgcaggctc cgcgctatat ctacaaactt gagcaggcac tggacgcgat gtaaatcagt
    15061 cgcgtacttt gtccgctttc tcccgggctt cccgctcgag agagaaaata atccgctgta
    15121 attgccgctc caccgtcggg ctgacgttaa gaaaacggaa gctcagacgg ggagtggtga
    15181 tggtttcatt cttgccatca atcactttgc gctcgctgat ggagattaac tgggcgtcaa
    15241 agtgaaaaac accccattgc cccatgttga cttcaatctg agcgaagcgc ataccttcgt
    15301 gcaacccggc aggctttgct gtttccagta atgcgcccat gccgcctaac gacaaatcat
    15361 acaggcggaa acgtaacgta ctgttatccg ccagtttggt ctggcaaaaa taaggcggat
    15421 ggagtggggc ggagatgcgg aaatatcggc gtcgttgtac aaaccataag gtgggaggcg
    15481 gtacggtaat aaatgccgga agctgcaagt attcactctg ttgtagttgt tcaacagtaa
    15541 actcgacttt cgcaccctga gtttcggcgg taatggtaat gtgctgtgcc tttagtacgg
    15601 cgatgttgtc ttcggcttga ctgccgaaat ccagcaccag tttatccggg gttattacca
    15661 gtattttgct gatcagctgc ccgccattcc agctgatacg caaaggaatt gcggctttgt
    15721 gcaaatcgcg taacacgccc aggacggcta acggattttg tttcaggaac tgctcatggt
    15781 aatgactcac gcggaaaaac tcctgactga caaactgtct ttggtttatc ggtcacagtt
    15841 aacaaaactt aatacaaatg cgtgaatatt ttttacatgt tgttcttaaa tcagccgaca
    15901 aacgcccagc gtcgatcctg cccttgccta tacttagagc gttgacgtaa gcatttcttg
    15961 cgtccgattc atcgaaacga gggcctgaac atgggaatta ttgcctggat tatttttggc
    16021 ctgatagccg gcattatcgc caagctaatc atgccggggc gtgatggtgg tggatttttc
    16081 ctgacctgta ttctggggat agtcggtgcg gtggtcggcg gctggctggc gaccatgttt
    16141 ggcattggcg gctccatcag cggttttaat ttgcacagct tcctggtggc ggtggtggga
    16201 gctattctcg ttctgggcgt attccgcctc ctgcgaagag aataagattt tcataaggcg
    16261 gatagcgata cagatgccgc tatccgcttt cacatcagaa cgtatactcg acacctgccc
    16321 agaaattgcg gccttcttca ataaaccctt cactgtaggc atattgggta tcaaacaggt
    16381 tattgaccga cgcattgacg ctgaagccat gacctaaggt gtaatcggct cgaatgtggg
    16441 tcaccgcaaa accggcggct ttttgtgaac cgtcactgtt gctgtagctg gaggaacgcg
    16501 cctcttccga cagcgttacg cttaacggct cccacggttt gagagtcatc catgcggtca
    16561 ttgtctgcgt tggcagatcg gttatcttgc cgatgtcttt gcgtttggcg tcagcgtgga
    16621 tcagggcgta gctcaatcct acatccagta tattgctgat tttcccctta ataccggcat
    16681 ccagaccgct gtaatccacc gtgccgctgt tctgattttg aatggtatcg gcatcgatat
    16741 tgtgcgagag gatggcatca ctcacccggt tatagtaaac gctgacctca aatccccagt
    16801 cgcgcgtgaa ggcaccattc caggttaaat ccaccccgcg agcgcgttcc ggcttgagct
    16861 gcgggttaac aatcgctatc tggttatacg caggtttgga tgtggtatag cgttctttca
    16921 gcgtcggaaa gcgtgtgcgg tcatagtacg aaagcgccag cgtgtcttca ttgacaaagt
    16981 ggtatttccc catcacctgc cagttaaaag ctgactgatt gttgtcgtca taatgggtga
    17041 tgctgccatc tttctcgtgt tttttcgctt ctacgctatc gcgccagtca tagctgattc
    17101 cagccacaac atcgacatta tcggcagcag cccattgata ttcactggcg agcgaccagg
    17161 tacgatcttc atagcgatcg taagcggcgt gcggcgcacc tttttcccgg tgtacgtcat
    17221 ctttccagtt aacggcaaac gacagcagat cgttttcacg cacatcggct gccagttgta
    17281 atccggcacc gtcgctgtaa tcggaataat ggctgtagct gcctttttta tttttcaaat
    17341 cagccagcga gttgtacatc atcagcgtat tttcaaaggt gtcgcgatac agccgacttt
    17401 tcagggtaaa acgatcgttt agttgggtcg ttccctgata ataaaaactt tctttgtcat
    17461 actctggcca ctgccagtag cgtgattttt gaccactatt tccgctgtat ggcgggttat
    17521 ctttttcacc atcctgctta atgtaagtca gtgtgtattc atcgttttca cgtggtgtaa
    17581 atccgagctt cacaatgccg cgtttatcat cagccgatga attaatcatc ttgccgtggt
    17641 tgcctgcaat atcattattt acaccatgcg gcaggccgag aaaatcctgc tttagctggc
    17701 taccgctgac ttgcaaatac cccagatcgc tgctggcggc aaatgaagca tgcatatcgt
    17761 aggcattgtc ctggctacgg ctccatccct ggcgatatcc cagacttgct tccagaggtt
    17821 ttgttggctt ctgggtggtg atattaatgg ctccgcccat ctgattaggc ccctgaagca
    17881 gcgacgaata ccctttggaa acttcgactg cccccagatt gttggtcaga atccgcgcca
    17941 gatcgaggtt gccgtcatag ggaacataaa tgggcacacc gtcgaaatag accggcacct
    18001 gccgactatc aaagccacga acttttacct gttcttcgtt gcggctgcct gacttttgca
    18061 gcaccacgcc ggggacgaca cttaatgcct gagcgacatt ctgtttatcc agggccttca
    18121 tggtgggttg atcgagaacg gtggtcgtcg tcgatgatac cggactggac catacggtca
    18181 gcgagtcgct ctcctgagcc gctacggcct gctgggtaaa ggcaagtgat aatgctgtgc
    18241 agagataatg ttttttgagt ctcatagaat aaattccgtt taacaaagaa ttaaatagag
    18301 gctgcgggtt gcagactgac tttaatgtca gccggtgccg cgtaataagg cgcggaggta
    18361 ataaaaaggc gaatgccgca gtcgaggtaa tttttgagtg tcgtcagatt aattccgccg
    18421 gtcagagcca gcgtgcagtg gggagccagc gacggcgcta tttgcgcaat ttcagttgcc
    18481 tgttgcggac taaatttgtc gagctgaagt acgtctggtt gcgcgcgtaa cgcggcgatt
    18541 gcctctttcg gcgtgtcggc ctcaacaaca attttcttct cgggtgcgtg gcgacgtaac
    18601 tgattgattg cgcctgacca gtcctgattg tcatgaagaa aatggcggtg gttggcaaac
    18661 aataatatgg tttccgcaca tccggcacga tgaatcagtc ctccggcagc cagaattgcc
    18721 tgcgaggcca gtaagcgggt gcccggaatt gcttttcggg tgcaggcgat attgccatca
    18781 ggataacgtt cacgaagtaa cgccagcatt tgatcgagat aatcagaaac accgcaactc
    18841 cactccagca cattctggat tgccttccat ccctgatgaa gtgctgcggc attaccctgc
    18901 gcacggatta gccgctgacc cgcgttcgct tgtgaaccgt cgctgaccgc gtcatcaata
    18961 gttaatccca gcgtagttaa catcttacac gcgacagaaa cgccgctgac gcagccgccc
    19021 tgacggagaa aaaactctat atagccatgc tggtgtccaa tatttaatgc tctcgtggtc
    19081 aggtcaccgc cctggatatc ttccagaagt aacgcatcga tttgtgcctg agaaaggaaa
    19141 atcatcgcag tgcctcctgc ggtacgctat cccaccagat gagcgcccag tcgcggctgg
    19201 caggggcgag ggcacgagca tcttgttgtt gataccagcg atataaccgc tcacgctcat
    19261 cgtcgttaag cgcgccaaga ctccagctga cgttctgctc aaaacgttcc caggtgctgt
    19321 tatcctgttg gcaattttgt ccacggataa aatcaacgtg ggcataaatt cccatctgat
    19381 acaggacgtt gagcgcaaag atatagttgg gtaattcgat cacttcccga ccagccgccc
    19441 gctgaatggc tggagagacg aacgaggtgc tgaccagatg agtcgtatag acgcgaagcc
    19501 gtgcctgatt attcagtttg ctcattgcct ggcgcatatc agccaccaga gtggagcggg
    19561 aagccacagc aatatcgcag cgtggcaggt cactccagtc ctcttcccag gcgcgctgga
    19621 tccagtggac attgtctgcc ttcagcgcgg cggcgcgacg ggcggcaacg ttcagcatcc
    19681 cctgactgta atcaacgcca taaatcgtcg tgagtttgtc agccagcgcc agcgatacgg
    19741 tacccggtcc gcagcccata tcgaataagg tctgagcgcc ttgtaggtca atttttgaga
    19801 tcagttgttg caggtagctg tccgtaggcg aggcgcaatt ttccgccatt ttttccgccc
    19861 gttgatccca gtgatccggc gttttttctg tgcggtgggc cagcttgagt tgctgcagat
    19921 aaaggtctgc aaaatcaata tcatcaatga gcatgatttt tccttagtgg ctaattgcag
    19981 agaggtgatg atgaatctgg tcggcagaaa cccgatacag ggcagccagt ttgtttgttg
    20041 ttaactgttc ggtcggtaag ccttgtgtta ctcgtccatc tggctctacc tggataatgc
    20101 tgtcggcaat ggcgttggca tggagcggat gatgagtcga cattaacatt gtcatgccgt
    20161 tatttttgag ctgtgccagc gtgtccagta gttggatctg atgaccaaaa tcgaggctgg
    20221 aggcaggctc atccagtaac aacaagcgcg gttgctggac gagtgcacgg gcgattaaag
    20281 ccagctgacg ttcaccgccg ctaagcgtat tccagcgtcg tgccgccaga tgcaaaatgc
    20341 ccagcttttc cagttgctcc gtggctttca ggcgttcttg cttaccggga acggaaaaag
    20401 cgcctatggt ggcggctagg cccatcaaca ccatatccag cacactaaaa gcaaaaatac
    20461 cgtcgtgcgc ctgcggcacc caggcaatag cctgcgcgcg ctggcgatgg ctgagtgtct
    20521 gcacagggag gctatccagt aagacctcgc cttttaataa cggtataacg ccaagaacgg
    20581 agcgcatgag tgtggttttg ccgcaaccat tggctcctaa cagacaacaa attgttcctt
    20641 gcgacagggt gaaggacacg tcgcgtagca ccggatggcg ggagtaaccc agagacgcat
    20701 gacggacggc aagcaaagtc actggcgacc tccgcgtaac aacaaaagca ggaaaaacgg
    20761 tgcgccaaca aaagcggtca gaatgccaag tgggatttct gttgttccaa tgctgcgtgc
    20821 cagcgtatcg gtaagcagga gcagaatcgc gccagtacac attgccatgg gcaacagctg
    20881 ctgatggtta tgaccggtca acaagcgggc aatgtgcgga acgaccaggc cgacccaacc
    20941 aattatgccc gctattgcta ccgtactggc ggtgattagt gtggcgcaga caatcaatcc
    21001 aaagcgcaga cgcgtcacgt tcagtcccag cgaacgggct tcgtcgtcgg aaagcgtgag
    21061 taaattcatg cgccagcgga gaaaaaacag gggcaaggag cctatgagaa tgatcggtgc
    21121 agcatagcag agatcgcgta aggtcacggt ggacagtccc cccagcaacc agaaggtaat
    21181 tgacggtaac tgggtgtaag gatcggccag cgttttaatt agtgatatcc ccgctccgca
    21241 caacgttcct aacgcaatgc ctaccagcac cagggttaag atcggatcgt ggcgggtcac
    21301 ccgacgcgta atcaaccaga cgcccgcgac caccatcagc ccgccgcaaa atgcgtacag
    21361 ctggatgtaa acaatggata atccccacag aattgccgtg caggcaccaa gtccggcacc
    21421 agcggagacg cctaaaatat ccggtgaaac taagggatta cgcaacattc cctgataggt
    21481 ggttccggct cctgccagcg ccgcgcccag taacagcgca gcaagaatac gcggcacgcg
    21541 gatttgccag aagacaattt gttcctgcac aggaacattt tcttgacgaa gaattagcgc
    21601 cagcagttgc tgcatatcga gatgataagc cccacttacg gcggcaaaag tgatactgat
    21661 agctgccagc agcaaaatac atccgtttac gattctcatt agctcgccac caatttttga
    21721 tagtcggcgt cactgagtga acaatgccag aacagttggg cgtaatgctg catgtcactt
    21781 ttaaactggc gattgatggc gggatccagc caggcgtgaa gtctgcgtaa tcccagaaga
    21841 cggttgattc ccggtggggc atccagccag ccaaagggca agccacttaa aaataagatg
    21901 cgttgttccg caacggcttt cacgccctgc cagagaggat cacgacgaat aaagtctgca
    21961 gtaacggcct cctgaaccag aataatatcc ggctgccagc gcaggagatt ttccatggaa
    22021 acctgtgtca ggccgtggcg atcggctatt tgcgcgacgt tgtgcaaacc taataattcc
    22081 gccgcctcgg tatgcaacga accctttaag cccgtttcca gccctcgagg accgcgcgca
    22141 gcataaaagc ggaggttagc ggcgggtgag gtggcgaatg cctgagcttc accaacgaag
    22201 cggctggcga gataggcttg ttctgcggcg cgatgctctt cgcctaacgt tttgccaagc
    22261 gttgttaatt gttctgctga ttgcgccagt ttcccgttaa gcaataacca gggtatttgt
    22321 gtctgttcgc taacctgccg tgcctgggag atccaggttt catccgtatt gccgcaatca
    22381 acgaccaaat cgggatgtaa cgccataagc ccttccagcg agagtgtgct ggcgcgcccg
    22441 gcgagtcgtc ccagcctggg taactggcga atgtgctctg gcaatggaat taatgcctga
    22501 cgggcaaaat caaaagagga aaaaccgacc attttttctg gcgcgacagc cagcaatagc
    22561 aaatcggcgg gcgcacctgc gctgacgatg cgtgtaatgt tttgggcttc gggaagcgtc
    22621 agtgaggccc agaatttgac tgattgtgca aaagaaggca gatgatgcag gaacaacgtc
    22681 gacgcaagaa tctgtgtaaa cctccgtctg g
//
LOCUS       DAZUCE010000066        22346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZUCE010000066 DAZUCE010000000
VERSION     DAZUCE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.066"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            210..428
                     /gene="ccdA"
                     /locus_tag="ACHY03_002103"
     CDS             210..428
                     /gene="ccdA"
                     /locus_tag="ACHY03_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFS3905334.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            430..735
                     /gene="ccdB"
                     /locus_tag="ACHY03_002104"
     CDS             430..735
                     /gene="ccdB"
                     /locus_tag="ACHY03_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFS3905335.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            736..1542
                     /locus_tag="ACHY03_002105"
     CDS             736..1542
                     /locus_tag="ACHY03_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFS3905336.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSPQLPVVIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKR
                     TGRTEKARIWEVTDRTVRTWIGEAVVAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            1721..2365
                     /locus_tag="ACHY03_002106"
     CDS             1721..2365
                     /locus_tag="ACHY03_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS3905337.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            2452..2760
                     /locus_tag="ACHY03_002107"
     CDS             2452..2760
                     /locus_tag="ACHY03_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFS3905338.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATVPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            3171..4151
                     /locus_tag="ACHY03_002108"
     CDS             3171..4151
                     /locus_tag="ACHY03_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFS3905339.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGGDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            4144..4560
                     /locus_tag="ACHY03_002109"
     CDS             4144..4560
                     /locus_tag="ACHY03_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFS3905340.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(4562..5836)
                     /gene="umuC"
                     /locus_tag="ACHY03_002110"
     CDS             complement(4562..5836)
                     /gene="umuC"
                     /locus_tag="ACHY03_002110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HFS3905341.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMHLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVESYSIDE
                     MFIDLREVNRCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKQRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPHKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(5836..6273)
                     /gene="umuD"
                     /locus_tag="ACHY03_002111"
     CDS             complement(5836..6273)
                     /gene="umuD"
                     /locus_tag="ACHY03_002111"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFS3905342.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(6270..6518)
                     /locus_tag="ACHY03_002112"
     CDS             complement(6270..6518)
                     /locus_tag="ACHY03_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFS3905343.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            6802..7110
                     /locus_tag="ACHY03_002113"
     CDS             6802..7110
                     /locus_tag="ACHY03_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905344.1"
                     /translation="MNYDGDGRATARFFSGKGCRHAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRIALPSERRRLRYSTVGLTRYRTR
                     "
     gene            7187..7870
                     /locus_tag="ACHY03_002114"
     CDS             7187..7870
                     /locus_tag="ACHY03_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFS3905345.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRFGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            7871..8092
                     /locus_tag="ACHY03_002115"
     CDS             7871..8092
                     /locus_tag="ACHY03_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905346.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            8052..8538
                     /locus_tag="ACHY03_002116"
                     /pseudo
     CDS             8052..8538
                     /locus_tag="ACHY03_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            8583..9353
                     /locus_tag="ACHY03_002117"
     CDS             8583..9353
                     /locus_tag="ACHY03_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905347.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDISRLATLLISRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRNAVRSICYQVAEDTRRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRK"
     gene            9768..10193
                     /locus_tag="ACHY03_002118"
     CDS             9768..10193
                     /locus_tag="ACHY03_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS3905348.1"
                     /translation="MQYAKPVTLNVEECDRLYFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            10240..10659
                     /locus_tag="ACHY03_002119"
     CDS             10240..10659
                     /locus_tag="ACHY03_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFS3905349.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            10659..10850
                     /locus_tag="ACHY03_002120"
     CDS             10659..10850
                     /locus_tag="ACHY03_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905350.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            11888..12118
                     /locus_tag="ACHY03_002121"
     CDS             11888..12118
                     /locus_tag="ACHY03_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905351.1"
                     /translation="MTVSIVSPSAAAVKPRRHTRFRREDIPAPEIDPVLKAFGRHIAR
                     SFRRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            12170..13531
                     /locus_tag="ACHY03_002122"
     CDS             12170..13531
                     /locus_tag="ACHY03_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFS3905352.1"
                     /translation="MTLSAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTISQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQRQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNSFRLVNVYITDMKITEI
                     PQK"
     gene            13578..14141
                     /locus_tag="ACHY03_002123"
     CDS             13578..14141
                     /locus_tag="ACHY03_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFS3905353.1"
                     /translation="MHSQLKERIRLMRARLDNAAPLAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRHVAPGAMCDAVEINSGLVRYLRDNFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPCGTFAYTRVPTMIIRLRA"
     gene            14141..14368
                     /locus_tag="ACHY03_002124"
     CDS             14141..14368
                     /locus_tag="ACHY03_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905354.1"
                     /translation="MTQGRREDKCPSARAGKRPRAGGAERGRADAPPLSLRGRSDGGF
                     CFFMPVICLLLMLLFLLLLLLLLLFFFFLLL"
     gene            14293..14538
                     /locus_tag="ACHY03_002125"
     CDS             14293..14538
                     /locus_tag="ACHY03_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905355.1"
                     /translation="MSVTDVVVPATATATATVLLFPAALILTTAVLSRLPGAPSAPGC
                     LAASGRQARLRQAAAGAPASLRRTRWRGFGLSLRSDA"
     gene            15011..15586
                     /locus_tag="ACHY03_002126"
     CDS             15011..15586
                     /locus_tag="ACHY03_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS3905356.1"
                     /translation="MVVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADATKKSGAKTKGRGRKAAQPEPQPQ
                     PPEGEDFQTISRSDWPDCDNRPVLCGASPER"
     gene            15589..15822
                     /locus_tag="ACHY03_002127"
     CDS             15589..15822
                     /locus_tag="ACHY03_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFS3905357.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDNGEHFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            15886..17850
                     /locus_tag="ACHY03_002128"
     CDS             15886..17850
                     /locus_tag="ACHY03_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFS3905358.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGSCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKDGAAFMAMMAEKARL
                     AALLPEGWARDMTTYLSLSQDVLLSLLSFCTACSIHGVQTREYGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIDALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCTPRPQAETETTEYRDDQAEAA"
     gene            17919..18353
                     /gene="psiB"
                     /locus_tag="ACHY03_002129"
     CDS             17919..18353
                     /gene="psiB"
                     /locus_tag="ACHY03_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFS3905359.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDEQGYSVSDIIHILMAEGGQA"
     gene            18350..19069
                     /locus_tag="ACHY03_002130"
     CDS             18350..19069
                     /locus_tag="ACHY03_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFS3905360.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSVLVMQKYSRQQAREAEQKARAYQTLVAQAEIELAFHSPE
                     TVGSWYARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            19066..19380
                     /locus_tag="ACHY03_002131"
     CDS             19066..19380
                     /locus_tag="ACHY03_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFS3905361.1"
                     /translation="MKWRYSLRWKLPSPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            19530..19682
                     /locus_tag="ACHY03_002132"
                     /pseudo
     CDS             19530..19682
                     /locus_tag="ACHY03_002132"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            19627..19749
                     /locus_tag="ACHY03_002133"
     CDS             19627..19749
                     /locus_tag="ACHY03_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3905362.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
     gene            complement(20203..20376)
                     /locus_tag="ACHY03_002134"
                     /pseudo
     CDS             complement(20203..20376)
                     /locus_tag="ACHY03_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20676..20978
                     /locus_tag="ACHY03_002135"
     CDS             20676..20978
                     /locus_tag="ACHY03_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905363.1"
                     /translation="MSAFSFNTAYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLLKQLWQINDELGELLDSVENHTPVIRKKASDLSALMMLFSQQAGQPMTMLLIC"
     gene            21134..21421
                     /locus_tag="ACHY03_002136"
     CDS             21134..21421
                     /locus_tag="ACHY03_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905364.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRTGLDIRAIC"
     gene            21539..>22346
                     /locus_tag="ACHY03_002137"
     CDS             21539..>22346
                     /locus_tag="ACHY03_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS3905365.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLREEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRLEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRGITGIDGDIRINKALWMIAEQFR"
BASE COUNT         5170 a         5719 c         6347 g         5110 t
ORIGIN      
        1 aaatcggatc tgccggaaat ggtccttcga taattacgct ttgtttttat aagtctgatt
       61 ttaattttta ttggttaaca taatgcaagg taaaataaat aaggcttact aaaagcaaga
      121 taacagtatg tgtattaacg cgctgatttt tgcggcataa gaatatatac tgatatgcat
      181 accagaagta tgttaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca
      241 gcgacagcta tcagctgctc aaggcgtatg acgtgaatat ctccggtctg gtcagtacga
      301 ccatgcagaa cgaagcccgg cgactgcgtg ctgaacgctg gcaggctgaa aatcaggaag
      361 ggatggcaga ggtcgccagg tttattgaaa tgaatggctc ttttgccgat gagaacaggg
      421 actggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
      481 gtggatgtcc agagtgacat tattgacacg cccgggcgac ggatggtgat ccccctggcc
      541 agtgcccgcc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatgtcggg
      601 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
      661 gaagaagtgg ctgatctcag ccaccgggaa aatgacatca aaaatgccat taacctgatg
      721 ttctggggaa tataaatgtc aggctccgtt atacacagcc agtctgcagc catggtaccg
      781 gcagtgtatt ctgccggaca gtctccacaa cttcctgttg tcattgatta tccggcagct
      841 ctggcacttc gccagatgtc gatggttcat gatgaactgc cgaaatatct gctggctccg
      901 gaagtgagtg ccctgctcca ttacgtcccg gatctgcgcc gcaagatgct gctggccaca
      961 ctgtggaaca ccggtgcgcg cattaatgaa gcactggcgc tgacgcgggg ggatttttcg
     1021 ctcgcgcctc cgtatccgtt tgtgcagctt gcgaccctga aacaacggac cgaaaaagcc
     1081 gccaggacgg caggaagaat gcccgccggt cagcagactc accggctggt tccgctctcc
     1141 gactcctggt acgtcagcca gctgcagacg atggtggcca cactgaaaat ccccatggaa
     1201 cggcgtaata aacgaacagg caggacagag aaagcgcgga tctgggaagt gacggacaga
     1261 acggtcagga cctggattgg ggaagcggtt gtcgccgctg ccgctgatgg tgtgacgttc
     1321 tctgtcccgg tcacgccaca tacgttccgc cattcctatg cgatgcacat gctgtatgcc
     1381 ggtataccgc tgaaggtcct gcagagcctg atggggcata agtccatcag ctcaacggag
     1441 gtctacacga aggtgtttgc gctggatgtg gctgcacggc accgggtgca gttttcgatg
     1501 cctgagtccg atgcggtgtc tatgctcaaa agaattcctt aagctctgac ttttttaaat
     1561 ccccttagga tatctcatgg atatattatc ggtatttcat attctgttga catgcaggac
     1621 cggtatagag taaaaaaact ctattgagta cctcttggat atccgaaagg tgtcttttgg
     1681 gttctttatg ggtattacat gtggattctg gagatacatt atgcctgtta ttgctaatac
     1741 acatcccaaa ggtggtgtgg gtaagacaac ttcatccgta aacattgttg gtgaaatgaa
     1801 gtctgataca gttgatctgg atactcatac cgggctttct atcattctgg ggctgagacc
     1861 tgaaggaaaa gaaatttccg tgaaagtacc caaaacagtg gatgagttaa tcgaaattat
     1921 gactccctac aagaacagcg ataagacgtt acttattgac tgtgggggat ttgactcaga
     1981 tcttacccgt actgcgattg cctttgcaga ctgcgttatt gtcccttcaa aagactccct
     2041 gactgagcgt attggtttga tgcattttga tggagtactg gacgaaatca gctctatcat
     2101 ggggaccgat ataactgctc acctgtatct ctgtaaagtt aatcctaaca agaagaaatt
     2161 ccctaagctt gatgcaatcc tgccatcgtt caaacatctg aagctgatga agagccgcat
     2221 ttctgctcgt gcagaatttg aggatgtcat tgagactggg atgggaatca ctgagtctgt
     2281 tcatgggcgc tattcggcag gaggtaagga agttattgcc cttatcgaag aaatcaatca
     2341 tctgattgaa aataacaaac agtaaagtgt atctattggg tatccatata gtatcctttg
     2401 ggcttttaat ggatatcttt tgaatatcta acaaacatcc tgggttatct catgagcaaa
     2461 gcgaaattta gtgcacaggc tgaaaaactg aaaaacttgg tggattctgc cagattacaa
     2521 acttcttctt ctgccgtaac agcaacagtc ccgtctcagc caaatgaagg tgcagaggaa
     2581 gtttcaggtc gtcccgtggc cgccaggacg aaggctaaga atcttaaggc tataccgctg
     2641 tcgtattttg aagctcatgc ggaacttaaa gcgaccagta aaacatctct ggattttagc
     2701 tcatatatta ttgaggctat aagagagaag cttgaacgtg atggtgcaat aggtcaataa
     2761 acatcagata ccttaagggt atctgatgtg tgtcttttaa atgcgttata gggattcctt
     2821 gtgtacctaa agagtcccta ttagacatct ttctgtatat ggtgacatga ttcgtcactg
     2881 ctttcctttc ctctcagttt caccatttca ttcccttccc ttaacctgaa ccatgatttc
     2941 aaagatgttt ttatattgca tttatgtgtt gcatataaca ttcataatgt ttttgtatct
     3001 atgatttctc tggtcttaag gttgcatgtg caatatacat gcaatttatt gtgtttttat
     3061 gttgctttca ccctttcaga aagaccatct ttccattaaa attgaatata cattgcatgt
     3121 atgaagtgtg tttgtttttt ctatttgttt atgagcaacg gagactggtt atgaaaatct
     3181 ttattgatga tggttctacc aacattaaac ttgcctggtt ggaggatggt gacgtgaaaa
     3241 cgttaatcag ccctaacagc tttaaaccgg aatggtcttt tagtttactg gatgatgctg
     3301 ccccggctaa ttacgagatc gacggcgaga agttttcatt tgatccatta agtgctgatg
     3361 ctgtcgtaac gactgaaaca cgctatcagt acagtgatgt gaatgttgtg gccattcagc
     3421 atgcgctaca gcagaccggc ctgaaagcgc agcctgttga tgtcatcgtc acattgccga
     3481 tttctgaata ccttgacgcc aacaatcaga aaaataagca aaacattgaa agaaagaaaa
     3541 aaaatgtgat gcgtgaagta cgggtgcagg ggggggatgc atttgttatt cgcagtgttt
     3601 ccgttcttcc tgaaagtatt cccgctggtt tcagtgttct tgcggggctt gaggatgatg
     3661 aatccctttt gatcgtagat ctcggtggta caactcttga tgtgtcgcat gttcgtagca
     3721 aaatgacagg gattacaaaa acctggtgtg atccgaatat cggcgtgtcc ctgattacat
     3781 ccggggttaa ggaacagatg gctgtacatg ctaatacaag agtgagctca ttccaggctg
     3841 acaatatcat tgtgcaccgt aatgaacctg actacctttc ccgtcgcatt tataatgcgg
     3901 aacagcgtga gtctatcatt aatgttatca atgaacggca gaagttgctt attaaacgag
     3961 ttaatgatgt catcagccgt ttcaccgact atacacatgt aatgtgtgtt ggtggtgggg
     4021 ctgaaattgt ggccgaggct gtgaagaacc tgactaaggt tcctgatgag cgcttttatc
     4081 tgagctcatc cccgcagttt gatttagtca tgggaatgat aaaaatgaaa ggtggtgtga
     4141 ctaatgagtg atgaaaataa atccagacgc tgctcatttg aacttttccc ggatgaacgg
     4201 acaggagata agatagcaga tgagctcatt gcaaatgaaa aactgaagga gcggggacgt
     4261 ttcatgcgcg cgatgttagt tactggtgcg gcgtttgcag ctatagataa acgtcttcct
     4321 ttgctgatat cggagttact gacagaaaat acgactctgg atgatattaa taaggtgatt
     4381 tccagcgtaa taccaggtgc tttttcggtt gaaaagaaat tgcttgaatt actggaaaaa
     4441 caatctggtc tccatacgtc tgtggattgc agtactcctc ttacagagca gagtctgtcg
     4501 agaaatgacg gtgaagacca gaccagaaga aacgctgaaa atatgttcgg agatgactga
     4561 actaacccag cctggccagg ggaatatctc gccatttagt tgtataacac tgactcaaca
     4621 tttcccgttt catttgccat tcaggggcgg ttcctcgccc cgcaaaccac actttccctt
     4681 ttcccgactg gttcagttca tcgagtgttt tcattaactt ttcgctgttt ttatggggct
     4741 ggatctcatc aaataatccc ggctgcgcta tacccgatgg tgtgaaatca gccagcatga
     4801 ctcctgcctt catatagcgg tacccttcac gccagacatg gtttaacgct ctgcatgctg
     4861 cggcaataat gtcccggctg tcctgtgtgg gcaatggaag cttttccaca gcggcattgc
     4921 tgtaacaggg ttcttttact gcaaaggggg atgtccgtac aaatgtcgtc acctgccggc
     4981 aatactgacg ctccccacgt tgtttctctg cagcccgctc tgcatactga acaacagcct
     5041 ggtgcatggc atctttgtct gtgattcgtt caccaaaact gcgactacag acaatctgct
     5101 gttttgccgg tggtgcttct tccagggata tgcaggactc gccgttgagt tcgcgtaccg
     5161 tacgctcaag aatgacgctg aagtttttcc ggatgaatgc cgtgttagcc tgcgccagct
     5221 gcagtgctgt gttaataccc agcgcattca gcttttccgt cagtctgcgt cctactcccc
     5281 agacctcacc aactggctgc agccccagta gcttcaagat ccgattacgg ttttctgccg
     5341 tcagcgcgac cacaccggaa aactgtggcc attgctttgt tgcccactgt gcacttttag
     5401 ccagcgtttt tgtaggtgca atgcccaccc ccatggtgag tcctgtccag ctctttacct
     5461 gttccctgag ctgatgacca aaaacctccg gagagatgca acggttcacc tcccgcaaat
     5521 caatgaacat ttcatcaatt gagtaggact caactgcggg agaaagcgac tccagaacag
     5581 ccataacccg ttggctcatg ctgtggtaca gcgcataatt gctggaaaat acatgtattt
     5641 tcttctccag gtgcatttgt ctgacctgaa accagggctg ccccattctg atgccaaggg
     5701 cttttgcctc cgggctgcgc gcgatcacac agccatcgtt attgctgagt acgatgaccg
     5761 gttcgttacg aaggtccggg cggaaaactt tttcacatga ggcgtagaaa ctgttgatat
     5821 cagccagtgc aaacatcagc gtaactcccg cgtcctgtgt atcacgtgag tgacaacacc
     5881 aaaaatacag atgttttccg gatacagtgt gcggaactcc gggctgtctg aaaccggctc
     5941 cagggccggg cgtgggcgca acagcagtcg tttgacggtg aactcaccgt cgatctcagc
     6001 gataacgatg tccccgtgtt gtggtttttc ggccctgtcc actaccagca gatcaccatt
     6061 ctgcacgcca gcctggttca tcgattcacc gctggcgcgc agaaagaagg tggctgcagg
     6121 tctgctgatg caatagctgt tcagatccag ttcctgctca gcataatcag tggcaggaga
     6181 aggaaaaccg gcctggcaac gatcggcaaa caacggtcgc acgtaactgt catcgcctga
     6241 aggggcagcc ggacggtgat aaacggtact catgctgcag gcagccagct gtcgtcctgc
     6301 cagatttctt ccagcagaga caggatacgt tctttatcct tgtcgttgcg ggctcctgtg
     6361 acagatacag acggggcgct gccctggtct atacgcagcc agatttccgg gtagtgtggt
     6421 ttcagacgtt gttcgatttc attctgtagc gcctgaagtg tgcctgattt gaggtttttt
     6481 gtgctctggc ggtcgaaaag aatttcaatg cggatcatgt tctcatctcc cttttctttt
     6541 gttgtatgta tatacagtat tctaataact gaatccacac acagtcaaat gtcattattg
     6601 tgactaaaaa acgctcaatt ctctgagagt gtggctatat gcctgctgtg ggctgctgtg
     6661 tccagcttat ccacagcatt ttgtgcacgg ttctgtggac aaaatacctg gttacccagg
     6721 ccgtgccggc acgttaaccg ggctgcatcc gatgcaagtg tgtcgctgtc gccggcctcc
     6781 tcacccggtc acttacggtg gatgaattat gacggagacg ggcgggcaac cgcccgtttc
     6841 ttttccggta aaggatgtcg ccatgctcct ctttttactg cgccggctga cgcggcgcgg
     6901 cataaacgct gcctgtggtc agtgtcccgc gtccggcggg cacgggacgg gcgcttttac
     6961 cgctcccggc tggcgtcggt gacagtgtac gccagcccgt cgcccttttc tgatgaacgc
     7021 ccttcaagcc gctttcgcat agccttgccg tcagaaagac gacggctgcg gtattccacg
     7081 gtcggcctga cccgttaccg gacgcggtga acagcccaca ggcagcgggg aacgggcacc
     7141 acaggggggc cctcctggtg tcctctgtaa gagaaggagc gttgttatgt cccgttttgt
     7201 cctcggtaac tgcatcgatg ttatggcccg tattcctgat aacgccattg atttcatact
     7261 gactgacccg ccgtacctcg tcggttttcg tgaccgtttc gggcgcacca tcgccggcga
     7321 taaaaccgat gaatggctgc aaccggcctg taatgaaatg taccgcgtac tcaaaaaaga
     7381 cgcgctgatg gtcagcttct acggctggca ccgtattgac cgctttatgg cggcctggaa
     7441 acgcgccgga ttcagcgtgg cagggcacct cgtgttcacc aaaaactaca catccaaatc
     7501 tgcgtatgtc gcgtaccgcc acgaatgcgc ttacatcctg gcaaagggca gtccggcccg
     7561 gccccgtaac cctctgccgg atgtgctggg ctggaaatat tcaggcaatc gtcatcaccc
     7621 gacggaaaag cccgttacca gcctgcaacc actgattgag agcttcacac atccgaacgc
     7681 gattgtgctg gacccgtttg caggcagcgg ctcaacctgc gtggccgccc tccagtccgg
     7741 acgccggtat atcggtatcg agctgcttga gcagtatcac cgtgccggac agcaacgtct
     7801 ggctgccgta cagcgggcca tgcagcaggg ggccgcaaat gatgactggt ttatgccgga
     7861 ggctgcgtaa atgaactatg ccggacacga aaaactgcgc gccgaagtgg cggaggtggc
     7921 caatgccatg tgcgacctgc gtacaaccat gaatgagatg gagcagcggt acagctttaa
     7981 tgccgacacc ctgccggaac gtctggtgcg tcagacgctg tttcgcgcaa accgcctcct
     8041 gatggaggca tataccgaaa ttctggaact ggattcctgc ttcaaagatt gaggggagag
     8101 agtatgtacg gaacatgcga aacgctatgc cgggcgctgg cagcaaagta tccgggagac
     8161 acgccgctga tgctggttat ctggtccccg gaagaaattc aggccctcgc tgacggaatg
     8221 gatatttccc tgtccgatca tgaaatcagg accgtcctgg tgcgcctgga ggacatcccg
     8281 gaagaccagc ggactgagtc cggtatttct tccggcgtgg cgatggagat tatcagcaac
     8341 gtgagcgaaa accgccaggt gaccgtcccg gctgaactgc tggcgtccct gattcagacc
     8401 gccgaacagg cactgtggaa acgtgagtgg gccgcccgtg ataacggcct cgtcgtcccg
     8461 gaatgcgtca cccgccgtca ggcggtggtt aatcaggccc gcgccctgct gaaaaacaac
     8521 acacacgaaa acgactgatg ttatcgccgc cttcgggcgg cgattcaggg agaccattaa
     8581 tcatgaacaa aacgctgaat gcactggttt gtcgccacgc ccgtaacctg ctgctggcgc
     8641 agggctggcc ggaagagacg gatattgaac aactgaatcc gcactatccg ggctggatca
     8701 gcatttatgt gcgactggat atatcccggc tggcgacgtt acttatcagc cgccatgatg
     8761 gcgtactgcc gccgcacctg gcctccgcca ttcagaaact gaccggaacc ggagcggaac
     8821 ttgttctgtc cggcagccag tggcaggcgc tgccggtact tccggcagac ggaacgcagg
     8881 tgtctttccc gtatgccggt gaatggctga cggaggaaga aatcagggca gttcttgatg
     8941 cggtgcgcaa tgcagtacga agcatctgtt accaggtggc agaagatacg cggcgtatcc
     9001 gggcggcgct gaccaccacc ggtcagacgt tgctgacccg ccagacgcgc cgctttcgtc
     9061 tggtcgtgaa ggaaagcgat tacccctgct ggcttgatga agatgacgaa aatctgcccg
     9121 tggtgctcga tgccattctg aaccgtggcg cccgtttttc ggcggtggaa atgtacctgg
     9181 tctgtgagtg tgtggagcat atcctggcca gtggtctggt ctgtgatgtg ctgcgtatac
     9241 cggatgaacc gtcccgtcgc tggtttgaca gggatattct gcgggaggtt gttctggagg
     9301 cacgggatga aatccgcagc atggcggatg cactggcgaa aatccggaaa tgatgacgct
     9361 ggcggggaaa aatctccgcc ttttccagcc ggtccccgga cagagcaaaa ggatgtcgcc
     9421 gcaggctccg ctttacccgg ccatgcgggg catggccttg tgggttttca gttatgtggc
     9481 ctcagcgtcg tgtgcgggct gtgccgtgcc tccatcttag ccgggctggc ggggatgcaa
     9541 gggtaaaatg cacgcggcgg tcacccggtc cctccttccc gtctgccgtg attttccggt
     9601 cgttcacccg ctcagatttc gcggtctcac cctccaactc ccgtcagccg tgtgcgtcag
     9661 gctgcggctt cccttgcgcc cttgcatccc cgccttatcg cccggctttt ctggaggcac
     9721 ggcaccgccc ggtgtcgcgg aatatgtgaa caacggagga aaacgtcatg caatatgcga
     9781 aacctgtcac tctgaatgtt gaagagtgtg accgtctcta ctttctgcct tacctgtttg
     9841 gcaacgattt tctgtatgcc gaggcgtatg tgtacgcgct ggcgcaaaaa atgatgccgg
     9901 aatatcaggg agggttctgg catttcatcc gcctgccgga cggtggcggt tacatgatgc
     9961 cggatggcga ccgttttcat ctggtcaacg gtgaaaactg gtttgaccgc actgtgagtg
    10021 ctgatgccgc aggtatcatc ctgacttccc ttgtgattaa ccgccagttg tggctgtacc
    10081 acgacagcgg ggatgcagga ctgacccagc tttaccggat gcgcgatgcg cagttgtgga
    10141 gccacatcga atttcacccc gaatgtaacg cgatttacgc ggcactggac tgattaacgg
    10201 acggggcggc aatgccgccc ctgtaaagga gacgagaaca tgtactgtac tgttaaagaa
    10261 attatccgcg aagtgctgaa tacagacgtg ccggacagtg aatgtgtttt tgccgtggtg
    10321 ctgacccgcg gggatgtgcg ccacatagcc cgggactgga acctgtcaga cgatgagctg
    10381 gaaaccgtca tgcagcggct ggacgatgct tttgtatatg gcgcatgtga cagagttgtt
    10441 tccgatattg tcaatgaact gatggaggaa aagcgcgtaa accgtcttgt aacagtccct
    10501 gcggtgctgc tggaaaaagt gatggtgatg gctggtagtg aaatttatcg cctccatgct
    10561 gtagggagcg agaacggggg cgacggtgac gcatttgtca gggaagaacg tgagattatg
    10621 cgggtaatgc gtcaggcgct ggacggggag aacgggtaat gaatatcagc acggaaaccc
    10681 gcgaaattct gcgtaattac agggccgtga ttaatgcgcg gcgtcgtgaa atggggcaga
    10741 aaccgctcac caccgcgcag attgttgatg aaatctgtga ttttgtggcg aatcagcagg
    10801 cggttttcct cggtggtcac tatatccttc agggcagcag aaacaggtga tacagggcag
    10861 gcggtggagg ccgcctgcca gcccgcgcga aaacccctgg caggctggcg cctgccggaa
    10921 gggatgcggg ccacgctggc gcgtcgcccg ttacccttcc cgtttgtgat gtgtctgact
    10981 caggagtgtt tctttcttat gttatggaac tgttcctgat gttgttgttg ttcctttatc
    11041 tgctgctctg attctgacta ctgcagtgtt gttctgtctg ccgggcgtac cttcggctcc
    11101 cggctgcctg gcggcatccg gtcgtcaggc ccgactccga caggcagctg ctggcgcacc
    11161 cgccagtctc cgccgaaccc gctgacgcgg tttcgggctg tcgctacggt ccgatgcgtg
    11221 aaaacccccg gcttgcagcg accggaacgg tcgcgccacc cttgtcgcgc cgtggcctgc
    11281 gctccaggca tcccggcaaa aggccgccgg gatgtaagga gcaacgccgt ttcgccaacc
    11341 gcgcttcacg tcgttgcagc gggtccctgc tcattgcctg ccgggtgcca cacacccgta
    11401 tgagtccctg tcgccggggg catagacagc cacaacctgc cgcactgact gtccagggta
    11461 aatgaacagc ccacaaaatt ttttacccag aacctggtaa aaaattttgc gttctgccca
    11521 ggacaggtgc gtcaggccgt ggcagtgatg ccccttgcgg cgaaggttct caaacgggtg
    11581 aagttcatac cacagggaat acagcagcag gttcccgcga aacgtcaaaa ccgcggctcc
    11641 cgaaactcaa cgggtgatgt tctttaaaaa tcaggattta tttaaggctc cggcaatacg
    11701 catttattcg ctctgtcagc atccggctgg caggtaatga gaacgcagga acagaaggag
    11761 gaatacaccg gagaacgtca gacgcagata tgaaaaaagc aggtcaggga cctgcttaat
    11821 ccataccgca gagactgcgc gaacagtctt tacggcttca ttaattaacg tcataagagg
    11881 ctaaattatg acagtaagta ttgtttcacc ttccgcagcg gctgtaaagc cccgtcgtca
    11941 tacccgtttc agacgcgaag acatccccgc gccggaaatt gaccccgtgc tgaaagcctt
    12001 cgggcgacac atagcccgga gttttcgccg ggggcgtggt gtacacatcc ccgccatgaa
    12061 aaacacggcc ttcgggcagg tgctccgcac actcgaactg aaacgcgctt ttaactgatg
    12121 aggtgagccg ccccgcgagg ggcggcaaca gacggaagga gaacatttta tgacattatc
    12181 agcaatcacc atgaccgccc cggaagccgc cagcccggtg cagatgtacc gcgcgaccta
    12241 ctcaccggat gataacaaac ttcgcctgta tgccgtgtca cgcctcgacc cggagacgta
    12301 taaaaaagtg catgatgccg gtttccgctg ggcacccaaa caggcgctgt ttgtggctcc
    12361 agcctggaca ccgggccggg aagacgtgct cctctcactt gccggagaga ttgaggatga
    12421 agacagcacg cttgctgaac gtcaggaagc acgggcggag cggtttaccg gatacagcgg
    12481 aaagcgggcc agtgaatccg cacaggcact tgatgaagtg gaacgactgg ccgcgatgat
    12541 cccgcccggt cagcccattc ttgtggggca tcacagcgaa cgccgcgccc gtcgtgatgc
    12601 gcagcgtatt gaaaacggca tgaaacgcgc cgtgatgctc tttgaacgtg cggaatactg
    12661 ggaagaacgg gcgcggtcgg cactgcttca cgcgaagtat aaagaacgtc cggacgttcg
    12721 ctggcgtcgt atcaaaaaaa tcgaagctga tttgcgcaag gctgaaaaga ccatctcaca
    12781 gtcgcagaaa tatctgacga tgtggcgggc tgaatcgctg gatctgaata tggcaaaact
    12841 catcagcagt catgaccata tcagcgcctg tttcccgctg gatacgtatc cgcgcccggc
    12901 agaaaaaagc cagtatgaag gaagtcgatc gttatggtcg gccctggatg atgacatcat
    12961 caccacggag caggcccgcg aaattgcgat ccgctgtcat gaacggcaga tccagcgtca
    13021 acaacgctgg gttaaccact atcagaaccg cctgatttat gagcgtgcca tgctggacga
    13081 aagcggcggc gtggttaccc ggacacagga ttttgagccg ggcgggcagg ttttcagccg
    13141 gggcgagtgg ctgaccatca tccgtgtgaa caaaagcaac ggggcggtga gttcagtcac
    13201 aacgccgaat tacagttttc tcgggtacag cggcacgatg aaagtgacgc ccgatcgcat
    13261 tacggactac aaagcaccat cggcagaaga ggctgccgtc gccagccagg ccgcgaagcg
    13321 tccgccggta gtcaactatc cgggggaagg tttccgggaa atgacaaagg cgcagtgggc
    13381 cgccctgccc cgggactgta aggccgtgcg cagtgtggca gagacagaag accacggggc
    13441 ataccgctac cgccgcacaa tggacaatag tttccgcttg gtgaatgtgt atatcaccga
    13501 catgaaaatc acggaaatcc cgcagaaata aggtatatcc cccggtgaaa tccccggggg
    13561 atgacggaga taatgacatg cacagtcagt taaaagaacg tatccggctg atgcgcgcaa
    13621 ggctggataa cgccgcgccg cttgctgaaa tccgggctga atctcagctt tttgtgactc
    13681 ctgccccggt ctgtgatcgc ctggtgacgc tggcggagat cagcaaccgt gatcacattc
    13741 tggaaccctc tgccggcacc ggggccatac tgcgggcgat tcgtcatgtc gcgccggggg
    13801 ccatgtgtga tgcggtggag attaacagcg ggctggtccg gtatctgcgg gataatttta
    13861 acggtgtcag ggttcagtgt ggcgacttca tggaatggca gccggtgcag tattacagcc
    13921 ggattatcat gaatccgccg ttcagtcacg ggcaggatat ccggcatatc ctgcgggcct
    13981 tttccctgtt gcgtccgggt ggcgtgctgg ttgccgtctg tctgaacggg ccgcgccagc
    14041 aggagaagct gttaccgttt tctgacgtcc gcgaggagct gccgtgcggc acgtttgctt
    14101 atacccgtgt tccgacgatg attattcgtc tgcgtgcctg atgacacagg gcaggcggga
    14161 ggataaatgc ccgtctgccc gcgccgggaa aagaccgcgc gccggtggcg ctgaaagggg
    14221 ccgcgctgac gcgccgcccc tttcattacg cggacgttct gatgggggct tctgtttctt
    14281 catgcctgtc atttgtctgt tactgatgtt gttgttcctg ctactgctac tgctactgct
    14341 actgttcttc tttttcctgc tgctctgatt ctgactactg cagtgttgtc ccgtctgccg
    14401 ggcgcacctt cggctcccgg ctgcctggcg gcatccggtc gtcaggctcg actccgacag
    14461 gcagctgctg gcgcacctgc cagtctccgc cgaacccgct ggcgcggttt cgggctgtcg
    14521 ctacggtccg atgcgtgaaa accccgggtt tgcagcgacc ggaacggtcg cgccaccctt
    14581 gtcgcgccgt ggcctgcgct cctggcatcc cggcaaaaag acgccgggat gtaaggagca
    14641 acgccgtttc gcaaaccgcg cttcacgtcg ttgcggcggg tgcctgcccg ttgttgtgtc
    14701 cggtgccata cacctgttta agcccctgtc gccgggggct gatacagcca caacctgccg
    14761 cactgactgt ccagggtaaa tgaacagccc acaaaatttt ttacccaaaa cccggtaaaa
    14821 aattttgcgt tctgcccagg acaggtgcgt caggccgtgg cagtgatgcc ccttgcggcg
    14881 aaggttctca aacaggtgat ggtgaatacc ggagggaaca aagagcaggt ccccgcaaaa
    14941 cgtcaaaacc gcggctcccg aaactcaaca gatgatgggg gctgaaaaca tcaacagaag
    15001 gagacacatc atggtagttc gtggcattaa caaggtcatt ctcgtgggac gtctgggaaa
    15061 agatccggaa gtccgttaca tccccaacgg gggcgcagtg gcaaacctgc aggtcgccac
    15121 gtcagaaacc tggcgtgaca aacagacggg ggagatgcgg gagcagacag aatggcatcg
    15181 cgtggtgctg ttcggcaagc tcgcggaagt agccggtgaa tatctgcgca agggcgcgca
    15241 ggtctatatc gaggggcaac tccgaacccg tagctgggaa gataacggta tcacccgtta
    15301 cgtcactgaa attcttgtta agaccacggg caccatgcag atgctgggga gtgcaccaca
    15361 gcagaacgct caggcacaac cgcagcctca gcagaatggg cagccacaga gtgctgacgc
    15421 gacgaaaaaa agtggcgcga aaacgaaagg ccgtggacgt aaggccgcgc agcccgagcc
    15481 tcagcctcag ccgccggagg gggaggattt tcagacgata tcccgttctg actggcctga
    15541 ctgtgacaac cgccccgtcc tgtgcggggc atcaccggag agatgaggat gagcgaatat
    15601 ttcagaatac ttcagggact gccggacggt ccttttaccc gaaaacatgc cgaagccgtt
    15661 gccgcacagt accggaacgt ctttatcgag gatgacaacg gagaacattt tcgcctggtt
    15721 gtccgtaata acggtgcaat ggtctggcgt acctggaatt ttgaggacgg tgccgggtac
    15781 tggatgaacc atgtcatcag ggatttcggc attcttaagt aagagaggtg ccggacgggt
    15841 aggcgtccgg cagacataca accaccccgg tcaaaggaga aatatatgtc agttacagag
    15901 tctaaggcaa aaacagagcg caaatccagc cgtaaacctg caaaaacgca ggaaactgtc
    15961 ctgtctgccc tgctggcgca gacggaggaa gtgagcgttc cgctggcttc gctgattaaa
    16021 tcaccgctga atgtgcgcac ggtgccgtat tctgtggagt ccgtcagcga actggcagag
    16081 tccattaaag gtgtcggtct gctgcagaat ctggttgttc atgccctgcc tggtgaccgt
    16141 tacggtgtcg ccgcaggtgg tcgccgactg gcagcactca acatgctggc agagcgtggc
    16201 atcattccgg ctgactggcc tgtccgcgtg aaggtcatcc cgcaggaact ggcgactgct
    16261 gcatcgatga ccgagaacgg tcatcgtcgg gatatgcacc cagccgaaca gattgccgga
    16321 ttccgcgcaa tggcacagga aggcaaaaca cctgcgcaga ctggcgacct gctgggctat
    16381 tcaccccgcc acgtccagcg aatgctgaaa ctggcagacc ttgcacctgt catccttgat
    16441 gcgctggcag aagaccgcat caccacagag cactgtcagg cgctggcgct ggagaacgac
    16501 accgcgcgtc aggtgcaggt gtttgaagca gcctgccagt cgggatgggg tggtaaaccg
    16561 gatgtgcggg ttatccgcaa cctgattacg gaaagtgaag tggcggtggc ggggaacagt
    16621 aaattccgct tcgtgggggc tgatgccttc tcgccagacg aactgcgcac cgatttgttc
    16681 agcgatgacg gggacggtta tgtcgaccgc gtggcgctcg atgccgccct gctggaaaaa
    16741 ctccaggctg tcgctgaaca ccttcgggaa gccgaaggct gggaatggtg cgccggacgt
    16801 atggagcctg tcggttcctg ccgtgaggat gccggaacat accgcagcct gccggagccg
    16861 gaagcggtgc tgacggaagc tgaagaagaa cgcctgaacg aactgatgac gcgctacgac
    16921 gcgctggaaa accagtgtga ggaatctgat ctgctggaag cagaaatgaa gctgatgcgc
    16981 tgcatggcga aggtcagggc gtggacgccg gagatgcgtg ccggaagcgg tgtggtggtg
    17041 tcctggcgtt atggcaacgt gtgtgtccag cgtggtgtgc agttgcgcag cgaggatgac
    17101 gcggctgccg acgctgaccg cacggaacag gtgcaggaga aagcgtcagt ggaggaaatc
    17161 agtctgccac tgctgacgaa aatgtcttcc gaacgcacgc tggcagtcca ggcggcactc
    17221 atgcagcaac cggacaaatc tctggcactg ctggcatgga cgctctgcct gaatgtgttt
    17281 ggcagcggag cgtacagtaa accagcacaa atcagcctgg aatgtaaaca ttattcgctg
    17341 accagcgatg cgccatcggg gaaggacggt gccgcattca tggcgatgat ggcagaaaaa
    17401 gcccgtcttg ctgccctgtt accggaggga tgggcacggg acatgacgac gtacctgtca
    17461 ctcagccagg acgtgctgtt atcactgctc agtttctgca ccgcgtgcag catccacggc
    17521 gttcagaccc gcgagtatgg tcacacgtca cgcagtccgc tggactcgct ggagagcgcc
    17581 atcggctttc acatgcgtga ctggtggcag ccgacaaaag caaacttctt cggtcacctg
    17641 aaaaagccgc agattatcga tgccctgaat gatgccggac tgtccggtgc cgcacgggac
    17701 gcggagaaga tgaagaaagg cgatgccgct gaacatgcag agttccacat gaaagacaac
    17761 cgctgggtgc ctggctggat gtgtacacca cgtccacagg cagagacgga aaccacggag
    17821 taccgtgacg accaggcaga agccgcctga ccgccagtca taaaccattt atcacgccgc
    17881 cccgccggag acggggcggc agcaagggag ataccgtgat gaaaactgaa ctgaccctga
    17941 atgtattaca gaccatgagc gcacaggaat atgaagatat ccgggctgcc ggaagtgatg
    18001 aacgccgtga gctgacacac gccgtgatgc gggaactgga tgcgccggac aactggacga
    18061 tgaatggcga gtacggcagc gagttcggcg gctttttccc cgtccaggtc cgtttcacgc
    18121 ctgcccacga acgttttcac ctggcgttat gttcaccggg cgatgtctct caggtctggg
    18181 tgctggttct ggtgaatgcc ggtggtgagc cgtttgcggt ggttcaggtg cagcggcggt
    18241 ttgcaccgga agctgtcagc cattcactgg cactggcggc atcactggat gaacagggat
    18301 acagcgtcag tgacatcatc catatcctga tggcggaagg aggtcaggca tgagcgcacg
    18361 ttcacaggca ctggttcctc tcagcacaga gcaacaggcc gcatggcggg cggtggcgga
    18421 gacagaaaaa cgccgccacc agggtaacac gctggcggaa tatccgtatg cgggggcgtt
    18481 tttccgctgt ctgaacggaa gccgcaggat ttcgctgtct gacctgcgat ttttcatgcc
    18541 gtccctgacc gcagaagaac tgcgcggcaa ccgttctcag tggctgtatg ccgtcgatgt
    18601 gctgattgaa acacagggcg aggtctgcct ccttcccctt cccggtgatg cagcagagcg
    18661 gttgttcccg tcggtgcggt tccgtgtcag ggaacgcagt cgccataaat ccgtgctggt
    18721 catgcagaag tacagccgcc agcaggcacg ggaggcagag cagaaagccc gtgcgtacca
    18781 gacgctggtc gctcaggctg aaatcgaact ggcgttccat tcaccggaaa cagtcggaag
    18841 ctggtatgca cgctggagtg accgggtggc agagcatgac ctggaaaccc tgttctggca
    18901 gtggggtgag cgttttccgt cactggccgg gatggaacgc tggcagtggc aggatatgcc
    18961 attctggcag gtcatcgcag aggcaggtat ggcagcaagg gaggcgggtc acgctgtccg
    19021 tgagatggag cggtggatgg tgccgaacaa actccgggag gcggcgtgaa gtggcgttac
    19081 tcattgcgct ggaaactgcc ttctccctgt cctggcgagc atgaactggt ttcagaagtg
    19141 gtggaggcgg ggcagcctgc accggcatcc gttatgtcac gctgggttgc cggagcaggg
    19201 tatgcggtct gtctggattt tatttctgac cgcccggtgc gccgctggag cgaagaacgt
    19261 aaggctgcgg tacggcggcg gaatctggag aaacgtatca acaggcatgc accactgttt
    19321 gcggatgaac tgattgcccg tgaactggct gaacgaccgg attattttca gggaaagtga
    19381 gtgtggtcag tacgcaggta tatgggctat gatgtgcccg gcgcttgagg ctttctgcct
    19441 catgacgtga aggtggtttg ttatcctgtc gtgtggcaga aggacaaaag ccccgtagtt
    19501 aatttttcat taacccacga ggcctctgca tgtctagtcc acgtcagtat ggcctcttac
    19561 cgtgcaaatt gcaaggagga gtaaaccatg aaactaccgg gaaacgccct tatctggtgc
    19621 gtattaatcg tgtgctgcac gttgttgata ttcacactcc tgacccggaa tcgcctgtgc
    19681 gaagtccggc tgaaggacgg atacagggaa gttacggcaa caatggctta cgaatccggc
    19741 ggtaagtagc aacctggagg cgggcgcagg cccgcctttt cagggctgat gctggtctga
    19801 ctgcactgaa gcgcctttat aaaggggctg ctggttcgcc ggtggcccct ttctccttgc
    19861 tggcattgta gcagcatgaa caaccagact gtcaggaagg ccacttccct ccgggaacgg
    19921 gaatgaacgt gcgcctgcgg cgctcgttct tttcccccgc cttctctgtt tatggccagt
    19981 tgtcagaaat gacattacct ttcccgtgat tattctcttt ttcttcctgc tctgattctg
    20041 actactgcag tgttgtcctg tctgccgggc gcaccttcgg ctcccggctg cctggcggca
    20101 tccggtcgtc aggcccgact ccgacaggca gctgctggcg cacctgccag tctccgccga
    20161 acccgctggc gcggtttcgg gctgccgcta cggtccgatg cgtgaaaacc ccgggcttgc
    20221 agcgaccgtt ccggtcgcgc cgcctctgtc gcgccgtggc ctgcgctccc ggcatcccgg
    20281 caaaaagacg ccgggatgta aggcgcaacg ccgtttcgca aaccgcgctt cacgtcgttg
    20341 cggcgggtcc ctgcccgttg ttctgtccgg tgccattcac ctgtttaagc ccctgtcgcc
    20401 gggggctgat acagccacaa cctgccgcac tgactgtcca gggtaaatga acagcccaca
    20461 aaatttttta cccaaaaccc ggtaaaaaat tttgcgttct gcccaggaca ggtgcgtcag
    20521 gccgtggcag tgatgcccca tgcggcgaag gttctcaaac aggtgatggt gaataccgga
    20581 gggaacaaaa ggcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa ctcaacagat
    20641 gatgggggct gaaaacatca gatacaggag gaaacatgag cgcattttct tttaacaccg
    20701 cttatcagcc aacaggcgat cagcagaaag acgcaatcgc acaaatcgac atcatgcaga
    20761 atcgtgctgt tcaggcaaat cttgcatacc agagcagcct ggatgcggaa acgctattga
    20821 aacagttatg gcaaatcaat gatgaactgg gggaactgtt ggatagcgtg gaaaatcaca
    20881 cgccagttat cagaaaaaaa gccagtgatt tgagcgcact gatgatgctt ttcagccaac
    20941 aggccggtca gcctatgact atgttgttga tttgttaatg ataatcaggg gcaggaaatt
    21001 aagcgaaaga tgctctgccc cgggtttgca gcgaccggaa cggtcacgcc acccttgtcg
    21061 cgctgtggcc tgcggcggct cccgaaactc aaccgatgat gggggctgaa aaccagagag
    21121 aggagaaagc aggatgagta cccgaaatat ccacgttaac accgcgtcgt acacgcttct
    21181 tgttgccggg aagaaaaaaa acaccgggga agagtgggac gtcctggaat tcagcagcct
    21241 gactgagctg aaaaaatatc gcaaaagcca cccggaaaag atggccttca gctatagcta
    21301 cgcgctcagc cggggtgtgg atacgcagtt ccgccatatc aacattgcgg aagccgatca
    21361 cttcaaacag ttcctgcgtc agattaagcg taccggcctc gatatccggg cgatctgctg
    21421 accggatgtg atgaatatcc ctcctgattg ttgcaatctt gttaatggag accgggaggg
    21481 atgatattaa gcggaaaatt ttttattaaa tttattcttt cataaaggag tagctgttat
    21541 gcgattagct tcccgttttg gtcggtataa ttccattcgc cgtgagcgtc ctttaacgga
    21601 tgatgaatta atgcagtttg tgccttcggt attttccggt gataaacatg aatcccggag
    21661 tgagcgttat acatatattc caacaatcaa tatcatcaat aagttacgtg aagaaggttt
    21721 ccagccgttc tttgcatgtc agagtcgggt tcgggatttg ggacgccgcg aatacagtaa
    21781 acatatgtta cgtcttcgca gggaagggca tattaacggg caggaagttc ctgaaattat
    21841 cctgcttaat tcacatgatg gttcatccag ttatcagatg atccccggaa tttttcgttt
    21901 tgtctgcaca aatggtttgg tgtgcggaaa taattttggc gaaatccgcg ttccacataa
    21961 aggcgatatt gtcgggcagg ttattgaggg agcgtatgaa gtgctcggtg tctttgataa
    22021 ggtcactgaa aatatggagg cgatgaaaga aatacatctt aacagtgacg agcaacattt
    22081 atttggcaga gctgcactga tggtcaggta tgaagatgaa aataaaacac cagtgacgcc
    22141 tgaacaaata attactcccc gtcgtttgga ggataaacag aacgatctct ggacaacctg
    22201 gcagcgggtt caggagaata tgataaaagg tggactgtcg gggcgaagtg cctccggtaa
    22261 aaataccagg acaagaggca ttacgggtat tgatggtgat atccgaatca acaaagcatt
    22321 atggatgatt gccgaacagt tcaggg
//
LOCUS       DAZUCE010000067        22307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZUCE010000067 DAZUCE010000000
VERSION     DAZUCE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.067"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(24..419)
                     /locus_tag="ACHY03_002138"
     CDS             complement(24..419)
                     /locus_tag="ACHY03_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFS3905366.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARKLGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLAQKEEYLRGYSADILVMGDDWAGKFDD
                     FSCVCEVVYFPRTPSVSTTGIIEVIRGNA"
     gene            complement(422..856)
                     /gene="fliJ"
                     /locus_tag="ACHY03_002139"
     CDS             complement(422..856)
                     /gene="fliJ"
                     /locus_tag="ACHY03_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFS3905367.1"
                     /translation="MRQIINTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAASAESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(859..2196)
                     /gene="fliI"
                     /locus_tag="ACHY03_002140"
     CDS             complement(859..2196)
                     /gene="fliI"
                     /locus_tag="ACHY03_002140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFS3905368.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(2189..2899)
                     /gene="fliH"
                     /locus_tag="ACHY03_002141"
     CDS             complement(2189..2899)
                     /gene="fliH"
                     /locus_tag="ACHY03_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFS3905369.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALQETLTPESQGE"
     gene            complement(2903..3913)
                     /locus_tag="ACHY03_002142"
     CDS             complement(2903..3913)
                     /locus_tag="ACHY03_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFS3905370.1"
                     /translation="MSELTINKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEDVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQVQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(3891..5546)
                     /gene="fliF"
                     /locus_tag="ACHY03_002143"
     CDS             complement(3891..5546)
                     /gene="fliF"
                     /locus_tag="ACHY03_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFS3905371.1"
                     /translation="MNAQIKKLTQAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWSGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEDELSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDVPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAPNQITNGATPA
                     DENRSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQ
                     TTQLTALLNNAAGIDVQRGDNLTLSLLNFVPQVAPVEPVIPLWKDDSVLAWVRLIGCG
                     LLALLLLFFVVRPLMKRLTAVRAPVITPEPEAVSEPWIATPEDERKNVDLPSLPGDDS
                     LPSQSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(5551..5892)
                     /locus_tag="ACHY03_002144"
     CDS             complement(5551..5892)
                     /locus_tag="ACHY03_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFS3905372.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     NRIMQGALGHVDQFQQVAEQKQTAIDLGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(5907..6893)
                     /locus_tag="ACHY03_002145"
     CDS             complement(5907..6893)
                     /locus_tag="ACHY03_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFS3905373.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAEGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDVADRSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKQGIEVFS"
     gene            7303..8154
                     /locus_tag="ACHY03_002146"
     CDS             7303..8154
                     /locus_tag="ACHY03_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFS3905374.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPILFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKDHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVMTHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLAQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            8147..8518
                     /locus_tag="ACHY03_002147"
     CDS             8147..8518
                     /locus_tag="ACHY03_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFS3905375.1"
                     /translation="MSKQEDILAGDFNLTDEAVPTMKSPDAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            8515..9267
                     /gene="fliP"
                     /locus_tag="ACHY03_002148"
     CDS             8515..9267
                     /gene="fliP"
                     /locus_tag="ACHY03_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFS3905376.1"
                     /translation="MKRRTQLTLGLGLLALAPLAIAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDRISLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLTIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            9270..9542
                     /locus_tag="ACHY03_002149"
     CDS             9270..9542
                     /locus_tag="ACHY03_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFS3905377.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            9544..10326
                     /gene="fliR"
                     /locus_tag="ACHY03_002150"
     CDS             9544..10326
                     /gene="fliR"
                     /locus_tag="ACHY03_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFS3905378.1"
                     /translation="MRASDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPIPHDLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTTALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            10316..11455
                     /gene="flhB"
                     /locus_tag="ACHY03_002151"
     CDS             10316..11455
                     /gene="flhB"
                     /locus_tag="ACHY03_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFS3905379.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPQVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYASVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDQSRAAAPFVVAKGTDEIALYIRLVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            11439..13532
                     /gene="flhA"
                     /locus_tag="ACHY03_002152"
     CDS             11439..13532
                     /gene="flhA"
                     /locus_tag="ACHY03_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFS3905380.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGAGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            complement(13765..14262)
                     /gene="rayT"
                     /locus_tag="ACHY03_002153"
     CDS             complement(13765..14262)
                     /gene="rayT"
                     /locus_tag="ACHY03_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFS3905381.1"
                     /translation="MSEYRRYYLKGGSWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKR
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHT
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDFSAG
                     ERIIL"
     gene            complement(14438..15187)
                     /locus_tag="ACHY03_002154"
     CDS             complement(14438..15187)
                     /locus_tag="ACHY03_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS3905382.1"
                     /translation="MYLFSHDRLLRLGVFSLPLLLPLLASAATPHTSLSYAARQRMQN
                     RARVLKLYQAHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPGPLKLSDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            15500..16240
                     /gene="dpaA"
                     /locus_tag="ACHY03_002155"
     CDS             15500..16240
                     /gene="dpaA"
                     /locus_tag="ACHY03_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFS3905383.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(16211..16978)
                     /gene="yafJ"
                     /locus_tag="ACHY03_002156"
     CDS             complement(16211..16978)
                     /gene="yafJ"
                     /locus_tag="ACHY03_002156"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFS3905384.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(17184..17762)
                     /gene="lpcA"
                     /locus_tag="ACHY03_002157"
     CDS             complement(17184..17762)
                     /gene="lpcA"
                     /locus_tag="ACHY03_002157"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFS3905385.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            18002..20446
                     /gene="fadE"
                     /locus_tag="ACHY03_002158"
     CDS             18002..20446
                     /gene="fadE"
                     /locus_tag="ACHY03_002158"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFS3905386.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(20489..20935)
                     /gene="ivy"
                     /locus_tag="ACHY03_002159"
     CDS             complement(20489..20935)
                     /gene="ivy"
                     /locus_tag="ACHY03_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFS3905387.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            21116..21886
                     /gene="yafV"
                     /locus_tag="ACHY03_002160"
     CDS             21116..21886
                     /gene="yafV"
                     /locus_tag="ACHY03_002160"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFS3905388.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKPGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLW"
     gene            complement(21944..22307)
                     /locus_tag="ACHY03_002161"
                     /pseudo
     CDS             complement(21944..22307)
                     /locus_tag="ACHY03_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         4787 a         6078 c         6138 g         5304 t
ORIGIN      
        1 gctcaaaaat aaattttatt ttgtcatgca ttccccctaa tcacctcaat aatccccgtc
       61 gtcgataccg acggcgtacg gggaaaatag accacctcac agacacacga gaaatcgtca
      121 aacttccctg cccagtcatc ccccatcact aaaatatccg cactgtaccc ccgcaaatac
      181 tcctctttct gcgccagcga ttcctccaga aatacgccat ccacgcacgc cagcccggcg
      241 ataatcgcca tgcggtcatc ctgatggtaa accggtgccc tgcccttttt ggcgatgttc
      301 agggcgtcgg acgatacgcc gaccagcagg cgctcgccca gcttgcgggc gcgttgtaaa
      361 aggcgcagat gaccgacatg aaacacatcg aaggtgccga aggtgatgac cgttttcatg
      421 gctactccgc taatttgtgc cgcagccagc actgcacggc gatgtcgtcc accgcttttt
      481 gcgctttcgc cgtagcgctt tcctgctgct cgcggcgttt gtcgtccagc gtcagggcca
      541 ccactttctc ttcgcacgcc gccgctgtca ggtttttctg cattcgcgtg gctttgatgt
      601 tggccagcgt tttttcctgc tcctgccagg cgatcacctt gcgcaatgtg cctttgtagc
      661 cgctgacgtt tttcagcgac tccgccgacg ccgccgcgcc cgcagatgtt ttctcgacca
      721 ggtagcctaa tgccttgata ttgttgtcat agcctgcgca gatctgtttt tgctttgcca
      781 gctcgacggt tttgtctttg accgatttat cccgcaggcg ctgtaattgc gcgagggtgt
      841 tgatgatttg tcgcatagtt actgttctgc ctttttgaca ccaggaaaga ggatttgcag
      901 gcggctttgc accagttcga gggaggcggg ttcgcgcatc tcctggcgta aaaacctctc
      961 gatagccggg aacattttta ccgctttgtc gacgctggcg tccgcgcctg ccacgtagcc
     1021 gccgagcggg atcagcggtt tgatctccat ataggcggcg tagttttgct ttaacgcgcg
     1081 ggcggattgc tgatgttcca ggctagtgac ctggttcatg cagcggctga tcgactggcc
     1141 gatatcaata gccggatagt gccccgcttc tgccagcttg cgggttaata cgatatgccc
     1201 gtccagcacc gcgcgggcgc agtcgacaat cgggtcttgc tggtcgtcac cttccgccag
     1261 cacggtgtag atggcggtca tcgatcctgc gctttcgctg ttgcctgcgc tctcgaccag
     1321 tttggggatc atgccgaatg ccgatggcgg atagcctttg gtggcgggcg gttcgccgag
     1381 tgagagggcg atttctcgct gcgccatcgc gtagcgggtg agggaatcca ccagcagtaa
     1441 aacgtgatgc ccgcggtcgc gaaaccaggc ggcgatagag tggcacagtt cggtggcttt
     1501 caggcgcatc agtggcgatt catcggcggg ggcgaccacg acgatagact ttgccagccc
     1561 gtccgcgccc agcgagtggt cgataaactc tttcacttcg cgcccgcgtt cgccaatcag
     1621 cccgaccacc acgatgtcgg ctttggtctg gcgggtgatc atccccagca gcacgctttt
     1681 cccgacgccg ctgcccgcca tcagcccgac gcgctggcct ttgccgatgg tcagcaggcc
     1741 gttaatcgct ttcacgccca cgtccagcgg ttcgtccacc gaacggcggg ttaagggatt
     1801 aaccgagggc ggcagcggcg gcagaagatc gttgccgttc aggcggcctt tgccatccag
     1861 cggttcgccg agaccattca ccacgcggcc taaccagctt tcgccaatca ggatgtcgtg
     1921 ggctttttct tccgggaaga cgcgcgcgcc cgccatcagg ccgaccggct gtttaaacgg
     1981 cattaagtag gtgatgtcgc gattaaagcc cacggcctgc gcgtcaatca gcgtgtgatt
     2041 ggcgctttcg atgcggcaca gctgcccggt cattagcggg cagccgacgc tctccagcag
     2101 gatgccgtta aggcgcacca gccgaccggc gacgcgggct aatgggatcg attccagcga
     2161 gcgcaacgcg ttatcgaaag cagaaaaatc actcaccctg cgactccggc gtcaacgttt
     2221 cttgcagcgc ggtcatacac tgttccaggc ggtgctcgca gccgatatcc agttcggatt
     2281 tatcggtaat cacccggcat tcgcccggcg gtaaatccgg tgaaggcgtc agcccccatt
     2341 cgctgacctt ttccggcacc gcgtcgcgca ggcggttaaa ctcttccgta ctcagcatca
     2401 cttgcagggt ttccggcatc gccgggaagg ccgccagcgc ctcttccacc agcgccagca
     2461 actgcgtcgg ttgcagtgcc agttcgcagc ggatcacctg gcgggtgact ttttccacca
     2521 gttgcaacaa atcttcccgc tgtttacgtt cgatatgggc gagaaagtcg ttcactttac
     2581 cggtgatggc ttccagcggc tgcgccgcct caacaaactg tttgcgccct tcctgctgcc
     2641 cggcgaggct gccttcggtg tagccctggc gtcgcccctc ggcaaaacct tgctgatgac
     2701 cttcgctaaa gccctgctcc tgaccttcgg tcattccctg agcaaaccct ttttgcagcc
     2761 cttcctgaaa accgtccatc aactggcgct ggtagtcggc ggcgctaatg ctggcggtga
     2821 gcggttctcc ggtcaccgca cgctggcgcg gcgggaagcg gtgcaggcga tagcgcccgc
     2881 gcagtgtttc aatggtcatg ggttactccg ccgtctgttc tgcaaagagc tgtagctgaa
     2941 tttcgcccgc ctcttccagt tcacgggcga tacccatgat ttcgcggcgg atctgttcga
     3001 tacggctaac cggcaccggc cccagacgcg cggtgatcgc ttccagttgc tgcacctgac
     3061 gttttggcat taccgcgtaa atcgagcggc gcagcagggc ttcagtgcct ttcaacgcca
     3121 ccgcccagtc ttccatcggt acttcatcga gcaggcgtcg gcgtacctct tcgttctgac
     3181 ggctgaggat aaagaagtcg tacatctcat cctggagctg ctccagcacg tcttcgtcgc
     3241 gctcgcgcat ctggtcgagg atcacctgct ggttgccctg gaagcggttg acgatatccg
     3301 ccgcctgttt gatgcctttc accttcgagc catgttccga aagcaccgac aggccgcgtt
     3361 cgatcaggcg atccagctca tccaccacgt cgcggttgac gtcgttgagt ttcgccaccc
     3421 ggtagagaat ttcgttttgc accgattcgt tgagatacga gagtaccgcc gccgagattt
     3481 ccggcgtcag aaacgcgagg aataccgcct gcaattgcag atgttcttcg gagatcagca
     3541 tcgccagctg gcgtggttcc acccattgca ggcgcgccat ccgcgagcgg atctcatcgc
     3601 cgtagatgcc gttgatcacg ctgctggcga tctcggtgcc gagcgctttg ttaagaatgc
     3661 cctgcaacat ggatcgcgat gcgccgttga tcccgctctg ttcgcggaac tcatcaaaga
     3721 agttattgat cactttgcgc gccatgctgg ttttgacgcc ggaaagccgc gccatgtttt
     3781 cgctcaggcg caccacttct tcgcggctga gtttttgcat caccgtcgcc gccgcttctt
     3841 cgcccagaca cagcagcagg atcgccgcct gttccaggta gctgttgctc ttatttattg
     3901 tcaattcgct cattgctggt aatccattgt ctgagaactt cggctacgcg atcggtgtcg
     3961 ctcatggcca gtttttgcag gaactcgagt ttcacttcga ggccggaact ctgggacggc
     4021 aagctgtcat caccgggtag cgacggcagg tcgacgtttt tacgttcatc ttccggcgtg
     4081 gcgatccacg gttcgctgac ggcttccggt tctggtgtga taaccggcgc acgtaccgcc
     4141 gtcagtcgtt tcatcaacgg gcgcaccacg aagaacagca gcaacagcgc cagcaggccg
     4201 cagccaatca gccgcaccca ggccagcacg ctgtcatctt tccacagcgg gatcaccggt
     4261 tcgaccgggg ccacctgcgg cacaaagttg agtagcgata aggtgagatt gtcaccgcgc
     4321 tggacgtcga tcccggcggc attgttcagc aacgcggtaa gctgcgtggt ctgctccggc
     4381 ttccagtttt tcagcgccgg ggcgttttga ttgagcacca ccgccacgtt gaggcgtttc
     4441 accgcaaagc cgggatgctg aatatgctgg acgctgcggt cgtaagagta atcgcgcttg
     4501 ctttcgctgt gtttggataa cgcttccggc gagcggtttt cgtcagcagg cgtcgcaccg
     4561 ttggtgatct gattaggcgc aaccggcggg cggttgctga gcgaaccggg cacgcccatc
     4621 gccacctggt tagtgtcgct gtccagcacg ctctcttcgc ggttgatttt cggcgcgtcg
     4681 ccatagtgtt cctgggtttc gtcgatggtg ctgaggtcga gatccggcat cacgctgacg
     4741 cggtaattcc cgctgccgac cagcgaatcc agcacgtttg ccacgctggc gcgggttttg
     4801 tcctgaatat ctttgaggat ctgatcgcgt ttacgggtag cggctgaaac cgcctcgccc
     4861 gcaccgatgc cgtcggtcag cagatttccc gcctgatcga cgacgctgac tttgctggcg
     4921 tgcagccccg gaatactgcc ggaaaccaga tgcacgatgg cgttcacctg gtccatgttc
     4981 agcttcgcgc catagtgcag gcgcaccact accgaggcgc tgttttgcgg cacatcgctg
     5041 accacaaacg agctttcttc gttgagcgcc agatgcaccc gcgcgctctc caccgcgtcc
     5101 agcgacatga tactctgcgc cagctcccct tcgaggctgc gtttgtagcg cacgttctgc
     5161 acgaactggc tgctgccgag cacttcgtct ttgtccatca gctcgtagcc gctgggcaat
     5221 atcgcctgca cgcctttcgc ggcgagagtc atgcgcgttt tcgacagttc atcttccggc
     5281 accagtatct gcccgctttg cgggtcgatg cgataggtga gcttttcgcc gtccagcacc
     5341 gtgacaatct gcgacacggg cagattttcc tggcgtccgt agagcgagac gtagccgcga
     5401 ttgccgctcc acagcacgct aacgataatc gccgtcgccg ccacggcaag ccccacaccc
     5461 gccatcagcg cccagcgttt gttatctgcg agacgcagtc gaaacgccgg aaacgcctgg
     5521 gttaacttct ttatttgtgc attcataggg ttagatagac atactcatca ggtcgttaaa
     5581 cccggtggcg attttgttgc gcacctgaac cagcgcggag aaagagagcg acgcctgctg
     5641 gctggcaatc atcgcgcctg ccaggtcatc gcttttaccc aggtcgattg ccgtttgctt
     5701 ctgctccgcc acctgctgga actggtcaac gtgaccgagc gcgccctgca taatacggtt
     5761 aaacgaaacg tccgggtcag tggaactgaa ggtcatggcg ggcagcaccg gcgcctgggc
     5821 gttggcctgc atccgctgca cgtcctgcat gatttgcgcc tgcatggtgc tgttgattgt
     5881 ggtaatactc atttttaact tccgggtcag gagaaaactt cgataccttg cttgcgcatg
     5941 gaggccagtc gatagcgcag tgcgcgcggg gtaatgccta acaggtcggc gattttgctg
     6001 cggttgccct gatatttacg catcaggtcg gcgatatatt gatattgcgc gcttcgtccg
     6061 tgctggccta aatcgctggt ttcggcaatg tggacggcgg gctgagcagg ccattgcagt
     6121 tcgctgcggt cggcaacgtc attttccggc aggcctaacg cgtccgggta aatcacgccg
     6181 tcgcggttta agatcattcc ccgctgaatg gcgttctcca gctgacgcac gttgccgggc
     6241 cagcggtaat tcagcagcgc ccggcgggta gattccgaca gggtgatatt tttcaccagt
     6301 accgtggaat attttttaat aaatgactct gccagcggga taatatcgtt caaacgttca
     6361 cgcagcggcg gcatggtaat gggaataacc gccagacgat aatagagatc ttcacgaaaa
     6421 cgcccttcgg cgacttcctg ctcaaggttt ttattggtac aggcaatcag gcgaaaattg
     6481 agtttaattt gtcggttgct gcctaatcgc tcaacctgtt gttcctgcaa tacgcgtaat
     6541 attttggcct gtaatgccag cggcatatcg ccaatttcat cgagtaataa ggtgccgtta
     6601 ttcgccagtt ccatttttcc tggcacgctg gcaattgcgc cggtaaatgc gcctttgtcg
     6661 tagccaaata atgtggcttc cagcatgttt tccggaatag ccgcgcagtt cacgccgata
     6721 tagggcgcgt tctcattttc accaaaagct acggtgtgaa tatatttcgc cacgcactct
     6781 ttgcccgcgc cggtttcgcc ctgaatgagc accggcacgt taaaagcggc gacacgcttt
     6841 gccagagtaa aagcgttaat gctggaagcg gcagttgcaa taagttcaga catagggtga
     6901 acgatataag tagcgacatt aatcaggatg ttcttaagcg tatcaccaga gggattaaca
     6961 agttcgaatt aaacgataat aaaaatatta ttcgtagtta aacaatgagt tacacgtaac
     7021 acaatgcctg tacccgaacg gtaggccgga taaggcgttt acgccgcatc cggcaattgt
     7081 gcaccgatgc ctgatgcgac gctgcgcgtc ttatcaggcc tacagttcgt gcaatgtttt
     7141 atgacgatgc ccgacatctc accctttaat aatttaatta ttttttcacc ctattaataa
     7201 tttgcccctt tcacgccctc tctatttaat acctcacctt gtgtcactgc cggatttgcg
     7261 tgcacaaaat cgcccatacc attacagcag agaaagtaag ccatgctgaa gtacagtaaa
     7321 acgccaggca tctttaaatt agaaggtaat cggctcggac gcccctacca tcgcctgccg
     7381 atcctgttta cggggaattt tgacgtgatt gattcgcatc ttggcagcta ctttctcaaa
     7441 aagcatcgca gcaatatcac cttaaaaaag attggctgcg aaatggatat tattaataaa
     7501 aacgccgaac taatggtttc ccaggtcggg catctggcgt ttgatattga tcgctcctta
     7561 ttgttaatgc tgctgggcaa tttttatggt ctggagtcct ctcttgaaga ggcgaaagac
     7621 cataatggcc tgccgaccaa aaccgaaacg cggttaaaga accgtctggc gctggatatc
     7681 tgcacgctga tttttaatct gcaaacctcc ggtattgcgc tgaaattaaa gctggacagc
     7741 agcacggtga tgacccactg ggcgtatcaa ttaaccttta ccctcgcggg cgatgaagag
     7801 agcagttttc gtattttgct cgacgacgcc cataccgatt ttattttaaa tctgatccgc
     7861 cacagcgagc acagtaaacc gcagcaggtg gcgaaatcgg tcaataaacc ggccctgatt
     7921 aaagaaatta tccgctccct gccgttgacc ctgaacgtca aaatcgccga gctgtcgatg
     7981 aatgtcgccg atctggcgca gataaaagcc ggggatattt tacccatttc actgggagaa
     8041 acgtttccgg tggcgattgg gcaatctgaa ttatttagcg ccctgattgt ggaagacaaa
     8101 gacaaactgt ttttgtcgga gctggcgggc aagaatgaga atagccatga gtaagcaaga
     8161 agatatttta gccggagatt ttaacctgac ggatgaagcc gttccgacga tgaagtctcc
     8221 cgacgccgaa acgctggtca cccgcctgga agaccgcttt tccgactcga tgacgctgct
     8281 aaaacgcatt ccggtgacct taacgctgga agtgtcgtcg gtggaaatta tgttagccga
     8341 cctgcttaac atcgacgacg acacggtgat tgagctggat aaactcgccg gggagccgct
     8401 ggatattaaa gtgaacaata tcctgctggg caaagcggaa gtggtggtgg tcaacgagaa
     8461 gtacggcctg cgcgtgctgg agtttaacac ccgcgatatc aacgacctgg caccatgaaa
     8521 cgccgcactc aactgacgct gggccttggg ctgctggcgc ttgcgccgct ggcaatcgcc
     8581 cagggcggcg atattgcgct gcttaacgtg gtgacccacg gcaacactca ggagtacagc
     8641 gtcaaaattc aggtactgat tctgatgacc ctcgtcggtt tgctgccgac gatggtgctg
     8701 atgatgacct gtttcacacg ctttattatc gtgctgtcgc tgctgcgcca ggcgctgggg
     8761 ctgcaacaaa cgccgccgaa ccgcatcctg attggcattg cgctctccct caccatgctg
     8821 gtgatgcgcc ctatttggct caatatttac gaccacgcgg tggtgccgtt tgaaaacgat
     8881 cggattagcc tgaccgatgc gttaagcacc gccgcgacgc cactcaaacg ctttatgctg
     8941 gcgcagaccg acaaaaaagc gatggcgcaa atcatgacca tcggaggcgc gaagggcaat
     9001 gccgccgacc aggatctcac gattgtggtg cctgcctatg tgttgagcga gctgaaaacc
     9061 gctttccaga ttggctttat gatttacatc ccgtttctgg ttatcgacct gattgtcgca
     9121 agcgtcctga tggcgatggg gatgatgatg ctgtcgccgc tgatcgtttc gctgcccttt
     9181 aagctgatgc tgtttgtact gatcgacggc tggtcgctga ccatcggtac gctaaccacc
     9241 agcattcgcg ggctggggct gggctgatta tgctgaccgt agacgtagcc gccgacattg
     9301 tcgccagcgg cataaaagtg gtgattttac tggtttcggt actggtcgtg ccaagcctgc
     9361 tggttggcct gctggtgagc gtgttccagg cggtgacgca gattaacgaa cagaccctga
     9421 gctttttgcc aaggctgatt gtcacgctgg tggtgctggg cgtgtgcggc aaatggatga
     9481 ttatccagtt gcatgatctc tgcattcatc tgtttaccca ggccgccctg ctggtgcatt
     9541 aagatgcgcg caagcgacgt tacgcaactg atggatctgg cgctggggct gtggtttccc
     9601 ttcgtgcgca ttatggcgtt tctgcgctat gtgccggtgc tggataacag cgccctgacc
     9661 gtgcgcgtgc ggatcatttt gtcgctggcg ctggcaatca tcatcacgcc gttgatcccc
     9721 catcccattc cccacgacct gctatcgctc aacagcctga tcctgacggt ggagcaaatc
     9781 ttatggggca tgttgttcgg gctgatgttc cagtttctgt ttctggcgct gcaactcgcc
     9841 gggcaaattc tctcgttcaa tatggggatg agcatggcgg tgatgaacga cccaagcagc
     9901 ggcgcatcga ccaccgtgct ggcagaatta attaacgtct acgcgatcct gctgtttttt
     9961 gccatggacg ggcatctgct gctggtgagc gtgctgtaca agggcttcac ttactggcca
    10021 attggcaacg cgctgcatcc gcaaaccctg cgtaccaccg cgctggcctt tagctgggtg
    10081 ctggcgtccg catcgctgct ggcgctgccg actaccttta tcatgctgat tgtgcagggc
    10141 tgctttggcc tgctcaaccg tattgcgccg ccgctgaacc tcttttcgct cggctttccc
    10201 atcaacatgc tggcggggct ggtttgtttt gccaccctgc tctacaacct gccggatcac
    10261 tatctgcatc tggcgaattt tgtgttgcag cagctcgacg cgctgaaagg tcactatggc
    10321 ggatagcagc agcgaagaaa aaacagagaa accctcggcg caaaagctgc gtaaagcgcg
    10381 cgaagagggg caactgccgc gctcgaagga tatggggctg gcggcgagcc tgtttgcggc
    10441 gtttgtggtg atctccagca gcttcccgtg gtatgccgat tttgtgcgcg agagttttat
    10501 cagcgtacat cagtacgcac aggagattaa taacccgcag gtgatcggcc agtttctacg
    10561 ccatcacctg ttaattctcg gcaagtttat tctcaccttg ctgccgatgc ccgccgccgc
    10621 gctgctctct tcgctggtgc ccggcggctg gctgttcttg ccgaaaaaaa tcctcccgga
    10681 tttcagcaaa attagcccgt taaaaggcat tgggcggctg ttttccagtg aacatctggc
    10741 ggaaacaggg aagatgacgg tgaagtcggt ggtggtactg gtaatgctgt gggtaagcct
    10801 gcgcaataac tttgcctcct ttctcggcct gcaggcgctg ccgttcaaac tggcgatgaa
    10861 tgagggtttg tcgctctacg ccagcgtgat gcgcaacttt gtgatcctgt ttattttctt
    10921 cgcactgatt gacgtgccgc tggcgaaagc cctgttcacc aaagggctga agatgaccaa
    10981 acaggagctg aaagaagagt acaaaaatca ggaaggcaag ccggaagtga aggcgcgcgt
    11041 acggcggctg caacggcagc tggcgatggg acaaatccgc aaagtggtgc cgaaagccaa
    11101 cgtggtgatc accaacccga cccactacgc cgtggcgttg cagtatgatc aatcgcgcgc
    11161 cgccgcgccg tttgtggtgg cgaaaggcac cgatgaaatc gctctctata ttcgcctggt
    11221 cgccgctgag aatcaggtgg aagtggtcga gttcccgcgg ctggcgcgct cggtttacta
    11281 caccacccag gtgaaccagc aaattccgtt tcagctttac cgggccatcg cccacgttct
    11341 gacctacgtc ctgcaaatga aacactggcg cgagggcgca cagccgcgcc cggccctgaa
    11401 cagacatatt tccattccca aagaggtcct taaactggat ggcgaaaaca actaaatcgt
    11461 tcctcgcgct gctgcgcggc ggcaatctcg gcgtgccgct ggtgatcctg tgtattctgg
    11521 cgatggttat tctgccgctg ccgcctgcgc tgctggatat tctgttcacc ttcaacattg
    11581 tgctggcggt gatggtgctg ctggtggcgg tgtcggcgaa acgaccgctg gaattcagcc
    11641 tgttcccgac cattttgctg atcaccacct taatgcgcct gacgcttaac gtcgcctcta
    11701 cgcgcgtggt gctgctgcac gggcatctcg gcgcgggcgc ggcgggtaag gtgattgagt
    11761 cgtttggtca ggtggtgatt ggcggcaact ttgtcgtcgg cttcgtggtg tttatcatcc
    11821 tgatgatcat caactttatc gtcgtcacca aaggggccga gcgtatttcc gaggtttctg
    11881 cccgcttcac cctggacgcg atgcccggca agcagatggc gattgacgcc gatcttaacg
    11941 ccgggttgat caaccaggcg caggcgcaaa cccggcgtaa agatgtcgcc agcgaggccg
    12001 atttctacgg cgcgatggac ggggcatcga agtttgtgcg cggggatgcc atcgccggga
    12061 tgatgattct ggcgatcaac ctgatcggcg gcgtctgtat cgggatcttc aaatacaacc
    12121 tgagcgccga tgccgccttc cagcaatatg tgctgatgac catcggcgac ggcctggtgg
    12181 cgcagatccc ttccctgctg ctctccacgg cggcggcgat tatcgtcacc cgcgtcagcg
    12241 acaacggcga tatcgcccat gacgtgcgcc accagctgct ggcaagcccg tcggtgctct
    12301 acaccgccac cgggatcatg ttcgtgctgg cggtggtgcc ggggatgccg cacctgccgt
    12361 ttttgctgtt cagcgccctg cttggcttta ccggctggcg gatgagcaaa cgcccgcagg
    12421 cggcggaggc ggaagagaaa agcctcgaaa cgctgacccg caccatcact gaaaccagcg
    12481 agcaacaggt cagttgggaa accattccgc tgatcgagcc catcagctta agcctcggct
    12541 acaaactggt ggcgctggtg gacaaagcgc agggcaaccc gctcacccag cgtattcgcg
    12601 gcgtacggca ggtgatatcc gacggtaacg gcgtgctgct gccggagatc cgcattcggg
    12661 aaaacttccg ccttaagccg agccagtacg ccattttcat caacggcatt aaggctgatg
    12721 aagcggatat tccggcggat aaactgatgg ccctgccctc cagcgaaacc tacggcgaga
    12781 ttgacggcgt gctggggaac gacccggcgt acgggatgcc ggtcacctgg atccagcctg
    12841 cgcagaaggc gaaggcgctg aatatggggt atcaggtgat cgacagcgcc agcgtgattg
    12901 ccacccatgt gaataagatt gtgcgtagct atattcctga tttgtttaac tatgatgaca
    12961 tcacgcagtt gcataaccgt ttgtcgtcga tggcaccgcg actggcggaa gacttaagcg
    13021 cggcgctgaa ttacagccag ttgctgaaag tgtaccgcgc gttactgacc gaaggcgttt
    13081 ccctgcgcga catcgtcacc atcgccaccg tgctggtcgc cagtagcaca gtgaccaaag
    13141 atcatattct gctggcagct gatgtgcgcc tggcgctgcg gcgcagcatt acccatccgt
    13201 tcgttcgcaa gcaggagctg acggtgtata cgctgaataa tgagctggaa aatctgctga
    13261 ccaacgtggt gaatcaggcg caacagggcg gcaaagtgat gctagacagc gtgccggtcg
    13321 acccgaacat gctcaaccag ttccagagca cgatgccgca ggtgaaagag cagatgaaag
    13381 cggcggggaa agacccggtg ctgctggtgc cgccgcagct gcgccctttg ctggcgcgct
    13441 acgcaaggtt gtttgcgccg gggctgcatg tgctgtcgta taacgaagtg ccggatgagc
    13501 tggagttgaa gattatgggg gcgttgagtt gattggcctt taaggggcgc atccggcatt
    13561 ggtgcacaat tgccggatgc ggcgtgaacg ccttatccgg cctaccgatc cggcacaggt
    13621 ttgtaggcat gataagacgc gccagcgtcg catcaggcat ccgcgcacaa ttgccggatg
    13681 cggcgtgaac gccttatccg gcctaccgtt aaagcacgat tcgtaggccg gataagcgta
    13741 agcacatccg gcaccagcgc aaaattataa aatgatacgc tccccggcac taaaatccgt
    13801 tatgtctccc gcccaatcaa tgggatataa ccctcgcgcc acatcgcgat ggaacgttga
    13861 gaatggccaa tcactcactt gctttaccca gccatgtttt actggattta tataaatata
    13921 atcaacatga tggcgatagt ctttggtatt gcggatggtg tgctcccaaa aacgtggttg
    13981 ccagatattt ttcaatccac aagcatgggt gaattgcttt ttaatctccc gccagcgaga
    14041 ggaaaaatca tcatcgcttt caggtaatgt ccagatacag tgcatatgct ccggcaaaac
    14101 tacccaggca ttaatttcaa aaggtctggc tcgcttaact ttaataatgg cgttacggag
    14161 cgtctggtac tgggtagtca aaagttggct tcgacgattc cgtaaattca ccgtgaaaaa
    14221 ccagcttccc cccttgaggt aataacgacg atattcagac atagcgactc ctttttctca
    14281 acggtaagaa gaaaaggaaa ggttggcaat gaggttgttt tatatcggga aagcggctcg
    14341 caaatattcg cgggctcgta ggcatgataa gacgcgccag cgtcgcatca ggcacctgcg
    14401 cacaaccgcc ggatgcggcg taaacgcctt atccgtccta caaaatcgtc tcttccgtca
    14461 agatccgccg cgcgcccaaa aaatgatcct gccagaaagg ttcagctaac cggcttaccc
    14521 gaatggtttc ccccgtacgc ggcgactcga taaattgccc atcgcccaga tacacgccca
    14581 tatggtcggc aatctcgcgg ctgtggatat ggaaaaaaag caaatctccc cggcgcaggt
    14641 cgttgtttgc cacaattgtt gcccgatggt agtgatacat ctcattggcc gtgcgcggga
    14701 gcttcgcctc gaggatcttg ttgtaggcat aaaagaccaa cccgctacag tcgaagcctt
    14761 tatcgggccg cgtgccgccc cagacgtaag gcttgccaag ctgctgctcc aggcggtgga
    14821 tcgccacttc agtgatgttg tgcaggtgat cgctgcttaa aaagtggttg ttttccgcca
    14881 gcgcctgcca gcgtcggtcg ctcaatttca gcgggccagg gaaccactcc gggtgctgtt
    14941 ttatctgctc acggttgtgt tggcgcagcg cccgtctgct ttcggcattg ccttccacaa
    15001 tatagctggc ctgttttttc agatgtgcct ggtacagttt gagtacccgc gcacggtttt
    15061 gcatccgctg gcgagcggcg tagctgaggg aggtgtgcgg tgtggcagcg ctggcgagta
    15121 aaggaagcaa aaggggcaaa gaaaacacgc ccaggcgcag caatctgtcg tgagagaaca
    15181 aatacatgga agatataaac cggctgaatg aagatgagaa tattagagga caggaaggag
    15241 aagcgggatt attaattgct tatttgcata ttactatcga ggtttcataa tcttaggaaa
    15301 taagaaataa atccgtttta catgcgcttt tcggactttt gaaaatcgct acttatgcta
    15361 aatttgtaaa cccagcgccg acagacttgc gtaaaactct tttggtgata atgtgagcct
    15421 tccaaatcca gactattccg atttgggatc cattttgccg tcctggcgtg tgtaaccgtt
    15481 ttatcaagga ataagcagta tgcgtaaaat cgcattaatt cttgcgatgt tgttgatccc
    15541 gtgcgtttcg tttgccggtt tgctgggcag cagtagctcg accacgccag tgagcaaaga
    15601 gtataagcag cagttgatgg gatcccctgt ctacatccag atcttcaagg aagaacgtac
    15661 gctcgatctc tacgtcaaaa tgggcgagca atatcaactg ctcgacagct ataaaatctg
    15721 taaatattcc ggcggcttag ggccaaaaca gcgtcagggc gatttcaaaa gcccggaagg
    15781 gttttatagc gtccagcgta atcaattaaa accagacagc cgttactaca aagcgattaa
    15841 tattggattc cccaatgcct atgaccgtgc gcatggttat gaagggaaat acctgatgat
    15901 ccacggcgat tgtgtttcca tcggctgcta cgcaatgacc aatcagggca ttgacgagat
    15961 tttccagttc gttactggcg cactggtatt tggtcagccg agcgtgcaag tgagcattta
    16021 cccgttccgc atgaccgacg ctaatatgaa gcgccacaaa tattccaact ttaaggactt
    16081 ctgggagcaa ctgaagccgg gttacgacta ctttgagcaa acccgtaagc caccgaccgt
    16141 ttctgtggtc aatggtcgtt acgtggtcag caagccgtta agccacgaag ttgtgcaacc
    16201 acagctggca tcaaactaca cgctccccga ggcaaaataa gcgccattcg cctggcataa
    16261 ttttttgcca ggtttcattg cccgtcagcg gctgtgtcgc aatcaccgtg accacatcat
    16321 tcggtgtggt ctgcgagctg aagtcgattt ccacatcctg atccagcaac gttgccacgc
    16381 caaacggcgc gcggcgggtg atccagtgta aattagtcga gcaatacgcc attacatagc
    16441 gcccgtccga aagcagcatg ttgaaaacgc ccttctgccg cagttcatcc gccagtgagg
    16501 cgatatattt aaataccgcc gccatgttgc ccggtgtgcg cgggtaacgc tgcgttaatt
    16561 tatgcaggag ccagcagaag gctttttcac tgtcggtttc gcctaccggg cggaagttgc
    16621 cggtttccag tgatttgtag cccgtcagtt gtccgttatg ggcataagtc cagttacgcc
    16681 cccataactc gcgggtaaat gggtgagtat tttccagcgc cacctcgccc cgattagcct
    16741 ggcgaatatg agccaccacc gaacaggatt ttatcgggta gtcctggacc agtttggcga
    16801 taggtgagtt aaagctgggt tgcggatctt taaatgtgcg acagccttta ccttcgtaaa
    16861 aggtaatgcc ccagccatct ttatgtggcc cggttccacc accacgctgc accagaccgg
    16921 tgaaactaaa gcagatatcg gtagggacgt tggcgctcat cccgagcaat tcgcacatgg
    16981 gtaacctcca caacagcggg agcgcatggc ccccggcgaa gattgattta agaattttca
    17041 ggtcggatgc ggcgtgaacg cattattcgg cctacggttc gcgtacagat ttgtaggcat
    17101 gataagacgc gtcagcgtcg catcaggcat cggtacacaa ttgccggatg cggcgtaaac
    17161 gtcttatccg gcctacgcca gacttactta accatctctt tttcaatcaa ctggatcagg
    17221 atatggatca ctttaatgtg aatctcctga atgcggtcgg cataaccaaa gtgcggtacg
    17281 cgaatttcga tatccgccgt gccagccatt ttgccgccgt ctttaccggt cagggtgatc
    17341 actttcattc ccttctcacg cgccgctgcg atcgctttga tcacgtttgc agagttaccg
    17401 gaggtggaga tccccagcag tacatcgcct tcgcgaccta ccgcttcaac gtaacgggag
    17461 aaaatatcat tgaaaccgaa atcgttaccg acgcatgaaa tatgactaac gtcagaaata
    17521 gcaatcgccg ggtagcccgg acggttttca cggtagcgac cggtcaactc ttcggcaaag
    17581 tgcatggcgt cgcaatggga gccgccgttg ccgcaggaaa gcactttgcc accggcttta
    17641 aagctgtctg ctaacaggac cgccgcgcgc tgaatggcgt gaatattggc gtcatctttt
    17701 aaaaagttag ccagcgtttc cgccgcttcg ttcagttcgt tacgaataag atcctggtac
    17761 atgaggatat ccttcagcat aaatgtaata gacaaaatgc agtgtaccgg ataccgccaa
    17821 aagcgagaag tacgggcagg tgctatgacc aggacttttt gacctgaagt gcggataaaa
    17881 acagcaacaa tgtgagcttt gttgtaatta tattgtaaac acattgctaa atatttttac
    17941 atccactaca accatatcat cacaagtggt cagacctcct acaagtaagg ggcttttcgt
    18001 tatgatgatt ttgagtattc tcgctacggt tgtcctgctc ggcgcgttgt tctatcaccg
    18061 cgtgagctta tttatcagca gtctgatttt gctcgcctgg acagccgccc tcggcgttgc
    18121 tggtctgtgg tcggcgtggg tactggtgcc tctggccatt atcctcgtgc catttaactt
    18181 tgcgcctatg cgtaagtcga tgatttccgc gccggtattt cgcggtttcc gtaaggtgat
    18241 gccgccgatg tcgcgcactg agaaagaagc gattgatgcg ggcaccacct ggtgggaggg
    18301 cgacttgttc cagggcaagc cggactggaa aaagctgcat aactatccgc agccgcgcct
    18361 gaccgctgaa gagcaagcgt ttctcgacgg cccggtagaa gaagcctgcc ggatggcgaa
    18421 tgatttccag attactcatg agctggcgga tctgccgccg gagttgtggg cgtaccttaa
    18481 agagcatcgc ttcttcgcga tgattatcaa aaaagagtac ggcggactgg agttctcagc
    18541 ttatgcccag tctcgcgtgc tgcaaaaact ctccggcgtg agcgggatcc tggcgattac
    18601 cgtcggcgtg ccgaactcat taggcccggg cgagctgctg caacattacg gcactgacga
    18661 gcagaaagat cactatctgc cgcgtctggc gcgtggtcag gagatccctt gttttgcgct
    18721 gaccagcccg gaagcgggtt ccgatgcggg tgcgatcccg gataccggga ttgtctgcat
    18781 gggtgaatgg cagggccagc aggtgctggg gatgcgtctg acctggaaca aacgctacat
    18841 tacgctggca ccgattgcga ccgtgcttgg tctggcgttt aaactctccg acccggaaaa
    18901 attgctgggt ggtgcagaag atttaggtat tacctgtgcg ctgatcccga ccaccacgcc
    18961 gggcgtggaa attggtcgtc gccacttccc gctgaacgta ccgttccaga acggaccgac
    19021 gcgcggtaaa gatgtcttcg taccgatcga ttacattatc ggcggaccaa aaatggccgg
    19081 gcaaggctgg cggatgctag tggagtgcct ctcggtaggc cgcggcatca ccctgccttc
    19141 caactcaacc ggcggcgtga aatcggtagc gctggcaacg ggcgcgtatg ctcacattcg
    19201 ccgtcagttc aaaatctcta tcggtaagat ggaagggatt gaagagccgc tggcgcgtat
    19261 tgccggtaat gcctacgtga tggatgctgc ggcatcgctg attacctacg gcattatgct
    19321 cggcgaaaaa cctgccgtgc tgtcggctat cgttaagtat cactgtactc accgcgggca
    19381 gcagtcgatt attgatgcga tggatatcac tggtggcaaa ggcattatgc tcgggcaaag
    19441 caacttcctg gcgcgtgctt accagggcgc accgattgcc atcaccgttg aaggggctaa
    19501 cattctgacc cgcagcatga tgatcttcgg acaaggagcg attcgttgcc atccgtacgt
    19561 gctggaagag atggaagcag cgaagaacaa tgacgttaac gcgttcgata aactgttgtt
    19621 caaacatatc ggtcacgtcg gtagcaacaa agttcgcagc ttctggctgg gcctgacgcg
    19681 cggtttaacc agcagcacgc caaccggcga tgccactaaa cgctactatc agcatcttaa
    19741 ccgcctgagc gccaacctcg ccctgctttc tgatgtctcg atggcagtgc tgggcggcag
    19801 cctgaaacgt cgtgagcgta tctcggcacg tctgggggat attttaagcc agctttacct
    19861 cgcctctgcc gttctgaagc gttatgacga cgaaggccgt aatgaagccg atctgccgct
    19921 ggtgcactgg ggcgtacagg atgcgctgta tcaggcagaa caggcgatgg acgacttact
    19981 gcaaaacttc ccgaaccgcg tggttgccgg gctgctgaat gtggtgatct tcccgaccgg
    20041 acgtcattat ctggcaccgt ctgacaagct ggatcataaa gtggcgaaga ttttacaagt
    20101 gccgaacgcc actcgttcac gtattggtcg cggtcagtac ctgacgccaa gcgagcataa
    20161 cccggttggt ttactggaag aggcgttggt ggatgtgatt gccgccgacc cgattcatca
    20221 gcggatctgt aaagagctgg gtaaaaatct gccatttacc cgtctggatg aactggcgca
    20281 caacgcgctg gcgaaggggc tgattgataa agatgaagcc gctattctgg tgaaagcgga
    20341 agaaagccgt ctgcgcagta ttaacgttga tgactttgat ccggaagaac tggcgacgaa
    20401 gccggtaaag ttgccggaga aagtgcggaa agttgaagcc gcgtaattga ataacggagc
    20461 cgaaaggctc cgtttcttta tccgctaatt atttaaaatt aaagccatcc ggatggtttt
    20521 ccaggctgcc ggtcaacgcc gcgaacagca ccgttttacc atcaatcgaa agcgcatcgt
    20581 tcacattcag ccaggtgagt ttctcttgcg acgttttctc atcaatagtc gagaacagcc
    20641 ccgtcatctg attagatttc tcggaccaca tcacagcgat acgttgcgag ccacagtcat
    20701 gcggtttgca cgcgctcatc acctgatacg tctcatctcc caacgttacg gtttgtgcgg
    20761 gagtataagt accgcctttc atcacccatg caggtagctt atgcccttgc accatctgat
    20821 taaacgcagc tttggtggtt tcgccctttg ccaggctgct aatggttaaa tcatcctgcg
    20881 ccattgcact ggtggcgatg accagtgcgg cgactgtcgt tattgcctta aacatcattc
    20941 ctcccgagct tatcctgccc atgttattaa cctcctaaag cctggcgata acacgataat
    21001 ttgcaaatca gatatttcca atatcagcat ttgttatcaa tttattctgc gctaaggcaa
    21061 ctgcctgata aggtgttaaa gttataaaac ccttcgcata aaggagttgt acaccgtgcc
    21121 tggtttgaag attacgcttt tgcagcaacc actggtgtgg atggatggtc ctgccaacct
    21181 gcgtcatttt gatcgtcaac tggaaggtat taccgggcgc gatgtgatcg ttctaccgga
    21241 gatgtttacc agcggctttg ccatggaagc ggcagcttcg tcgctagcac aagatgacgt
    21301 agtgaactgg atgacagcta aggcgcagca gtgcaatgcg ctgattgcag gcagtgttgc
    21361 attacaaacg gagtctggtt cggttaaccg ctttttgctg gttgagccgg gcggcacggt
    21421 acatttttat gataagcgtc atctgttccg catggcagat gagcatctac attataaacc
    21481 gggcaatgcg cgagtgattg tggaatggcg cggctggcgt attttgccgc tggtgtgcta
    21541 cgacttacgt tttcctgtgt ggtcgcgcaa tctcaacgat tatgacctcg ccctgtacgt
    21601 cgccaactgg cctgctccgc gctctctgca ctggcaggca ttgctgacgg cccgcgcgat
    21661 tgagaatcag gcgtatgtgg cgggatgcaa tcgcgtcggc agcgatggca acggctgcca
    21721 ttatcgcggt gacagccggg tgattaatcc gcaaggcgag attatcgcta ctgccgacgc
    21781 gcatcaggca acgcgcattg atgcggagct gtcgatgatg gcattgcggg aatatagaga
    21841 aaagtttccg gcatggcagg atgcggatga gtttaggttg tggtgagtgg gggttgtgtt
    21901 tagggccggg gagagtcggg gtaagactac gaaagcccgc tctcctgcga ccacttttat
    21961 cataggagtg tggaagcgct tttccatcaa ttgcgatgac gtctttatca tttgatgaat
    22021 ggcaatctcg catccagtta ataaagcatt cgtgaaattt tgcaggactg atacaggata
    22081 caactctggc aatggtatcg tgaacaggaa taccattttc aaaatcacca tattgcttca
    22141 aaaaatcgag atgtgtttcc ccaaaatcct ctatatcttc ccaaccttct gcaccagaaa
    22201 taacggcaca aatagtcaac agtagaatat ccgataattt atgttccacg ttccaggctt
    22261 gtctgtaatc ggggataaca gaaatatgtt ccatcaattt tttaagt
//
LOCUS       DAZUCE010000068        22082 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZUCE010000068 DAZUCE010000000
VERSION     DAZUCE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.068"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            12..2438
                     /gene="ynfE"
                     /locus_tag="ACHY03_002162"
     CDS             12..2438
                     /gene="ynfE"
                     /locus_tag="ACHY03_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFS3905389.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREEVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVYNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            2499..4922
                     /gene="ynfF"
                     /locus_tag="ACHY03_002163"
     CDS             2499..4922
                     /gene="ynfF"
                     /locus_tag="ACHY03_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFS3905390.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            4933..5037
                     /locus_tag="ACHY03_002164"
                     /pseudo
     CDS             4933..5037
                     /locus_tag="ACHY03_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            5463..6095
                     /locus_tag="ACHY03_002165"
     CDS             5463..6095
                     /locus_tag="ACHY03_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFS3905391.1"
                     /translation="MTSQLIPVFNGTIANETTLLVNARDLHTFLGVGKRFASWITERI
                     AEYGFVENQDYILVSPNREIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRNIQPVQTELQFTAEEIILLCYMQLWMEKAQDLSKHLYPIMKELNSSYTNK
                     LYDIAFETIYMVTKNRDALLREAARLDQSSFVVKRAIPMLKTLRARQFEF"
     gene            6143..6364
                     /locus_tag="ACHY03_002166"
     CDS             6143..6364
                     /locus_tag="ACHY03_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFS3905392.1"
                     /translation="MADIIDNAAEIEELQRNLSLQKYKSDSNAPSATHCCECGDPIDE
                     RRRQAVRGCRACASCQQDIELINKQRGVK"
     gene            6361..7068
                     /locus_tag="ACHY03_002167"
     CDS             6361..7068
                     /locus_tag="ACHY03_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS3905393.1"
                     /translation="MNGVSYQALRLAAENATPGEWCTDDYGVIANAGLNANYYIASCS
                     GPDNRANKRFIAAANPATVLALLDERERNQQYIKSRDQENEDIALTVGKLRVELEAAK
                     SKLNEQREYYEGVIADGSKRIAELEKSEEQLINERDHAESALADMYFAATGDRPEWSN
                     CFSFSDAVDAVVDRIADLEAKQPSPVVPEGLVKAVRFYEQVKRENPPAETGAWKDAVD
                     WVLKEACQVVNTGIKGE"
     gene            7070..7248
                     /locus_tag="ACHY03_002168"
                     /pseudo
     CDS             7070..7248
                     /locus_tag="ACHY03_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7582..7731
                     /locus_tag="ACHY03_002169"
     CDS             7582..7731
                     /locus_tag="ACHY03_002169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905394.1"
                     /translation="MITITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVA"
     gene            7735..8211
                     /locus_tag="ACHY03_002170"
     CDS             7735..8211
                     /locus_tag="ACHY03_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS3905395.1"
                     /translation="MVEDGGMCIDGFGEYVGHSLPDGTHQLYTAPPAPVVPEEKPMPN
                     PLKMYAVDAVAAIAEVRGWNSCRASMLHGAEPVSQTYKLNELSSNSPVTPARWISCST
                     RIPAQDDWILIYSKHGEYMAGQVQGEYVELSDGTLSWLGSALFWMPLPEPPKEVRQ"
     gene            8502..8657
                     /locus_tag="ACHY03_002171"
     CDS             8502..8657
                     /locus_tag="ACHY03_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3905396.1"
                     /translation="MMQQKAMLIALIVICITVVVTALVTRKDLCEVGIRTGQTEVAVF
                     VDYESEK"
     gene            8777..9121
                     /locus_tag="ACHY03_002172"
     CDS             8777..9121
                     /locus_tag="ACHY03_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFS3905397.1"
                     /translation="MKKVLIAALISGVSFGVFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGNERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            complement(9554..17938)
                     /locus_tag="ACHY03_002173"
     CDS             complement(9554..17938)
                     /locus_tag="ACHY03_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFS3905398.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VTTVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAEATDEWLTGK
                     MSPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEETAMRDAETVQQDDAA
                     PQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     AKRQAVMNELKAKPRSELLEEYRRLSQKEGRTETEEQQFQAIREVIRPQQEVTPEAQS
                     QPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMG
                     SDVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGR
                     QGETLEGEMVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPEL
                     AQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGA
                     AKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDI
                     PAKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLP
                     PSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAK
                     VWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSAS
                     DARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGFR
                     GVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAA
                     HYEPGEAAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDFMTEAQRARR
                     VFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAA
                     RAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRT
                     LKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDS
                     DLKVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGADASSQYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRSGAMTANIGRTPPVL
                     RHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVM
                     LLDAVDKNGDPVVSAVHMKATDKRIEINKVASVYGTKGGMNKANSLDKAGLTLYRKEK
                     LSRDNPQYSGLQLPKEERSYRGSLDKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQ
                     MQDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLL
                     ADYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQ
                     AQYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQNAYRYYVPLKGQDVDGVVSLPR
                     TGKGFTIGGRESKQAMGRASRAQSPSTQAIQDLSESLIRSRKNEVGNAFLKLVQDNPD
                     KDYWQVFTDDRPDTMRVIAERKDQETGETIREVVERPVAMAMMADRYFTTKKNGKTYY
                     IKLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAV
                     MNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKNLTGKGAQWQKVWKE
                     FVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVEN
                     ALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANL
                     VRTLGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYD
                     KVPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAA
                     GNVVGGILGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENIP
                     FGTPKPDSQLGIRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLIFWIDYISGGT
                     GRFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAEL
                     KSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSSLSLAQQAAMVK
                     SVERDMKVAVDRFNREYNKKVGVE"
     gene            complement(18008..19272)
                     /locus_tag="ACHY03_002174"
                     /pseudo
     CDS             complement(18008..19272)
                     /locus_tag="ACHY03_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19283..19555)
                     /locus_tag="ACHY03_002175"
     CDS             complement(19283..19555)
                     /locus_tag="ACHY03_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFS3905399.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGATIGATVGSAVPVVGTAVGAVAGAVIGGIAGALF"
     gene            complement(19565..20011)
                     /locus_tag="ACHY03_002176"
     CDS             complement(19565..20011)
                     /locus_tag="ACHY03_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905400.1"
                     /translation="MEYGKYETRARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNPQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            complement(20014..20670)
                     /locus_tag="ACHY03_002177"
     CDS             complement(20014..20670)
                     /locus_tag="ACHY03_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905401.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELASNLTRSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(20765..21118)
                     /locus_tag="ACHY03_002178"
     CDS             complement(20765..21118)
                     /locus_tag="ACHY03_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905402.1"
                     /translation="MQRVVRNSRNPEFMNEVKDACFKKQAFCFEAPDGFLVLRSVLSA
                     DGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHTARRGFIRLGKRLGFER
                     MPDDEDGFMVFRIAV"
     gene            complement(21254..>22082)
                     /locus_tag="ACHY03_002179"
     CDS             complement(21254..>22082)
                     /locus_tag="ACHY03_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3905403.1"
                     /translation="AYFVNTKTNQILFSADGEGVVTTVGADGKTYSRHVPEISEYIYE
                     IKMKSVNPLDSTIDTGLSAADVTDIHARLSSLGTGKTQVGGGATAGGENATAVGYNAI
                     ANHTNSVAVGANSQTTRPDEVNVGNRVIGGLRDGVQAADAATYGQLERYRIAEENARI
                     QGDAQTLKSANTYTDNRVSTLERSTNQQFRQLRDEVEKNRKRADAGIAGAMAMTAIPV
                     VEGKTYSFGMAASNYRDEQAIAAGMHFRTTENSAVRLNASWDTQNGSGVAAGMSVGW"
BASE COUNT         5294 a         6040 c         5309 g         5439 t
ORIGIN      
        1 gggagtgagt tatgtccaaa aatgaccgaa tggtgggcat cagccgcaga acactcgtta
       61 aatctaccgc gataggttct ctggcgctgg ctgcaggcgg tttttctttg ccgtttaccc
      121 tgcgcagtgc agcagcagcg gtacaacagg cccgcgaaga agtggtctgg ggtgcctgtt
      181 ccgtcaactg tggtagccgc tgtgcacttc gtctacatgt taaagataat gaagtgacct
      241 gggtggaaac tgacaatacc ggcagcgatg agtacggcaa ccatcaggta cgcgcctgtt
      301 tgcgcggtcg ctccatccgc cggcgtatta atcatcccga tcgcttgaat tacccaatga
      361 aacgcgtggg taaacgcggt gaaggcaaat tcgaacggat tagctgggat gaagccctgg
      421 atactatcgc cagtagcctg aagaaaaccg tcgaacaata tggcaatgag gctgtatata
      481 ttcagtactc ttcggggatc gttggcggca atatgacccg ctcttcgcca tcagcctcgg
      541 cggtcaaacg cctgatgaac tgctacggcg gttcactcaa tcagtatggc tcctacagca
      601 ccgcacaaat ttcctgtgcc atgccctaca cctacggcag taatgatggc aacagcacca
      661 cggatattga aaacagcaag ctggtcgtga tgtttggtaa caacccggca gaaacccgca
      721 tgagcggcgg tggcatcact tatcttcttg aaaaagcgcg cgaaaaatcg aacgcaaaaa
      781 tgattgttat cgatccgcga tataccgata cggctgccgg tcgtgaagac gaatggcttc
      841 ctattcgccc gggcaccgat gccgcgctgg ttgcgggtat tgcctgggta ttgattaacg
      901 aaaatctcgt tgatcaacct tttctcgata aatactgcgt cggctatgac gaaaaaacct
      961 tacccgcaga tgcacccaaa aatggtcact ataaagccta tattcttggt gaaggagacg
     1021 ataaaacagc gaaaacgccg cagtgggctt cgcaaattac cggtatcccg gtggaccgta
     1081 tcatcaaact ggcgcgtgaa attggcacag caaaacccgc ttatatctgc cagggctggg
     1141 ggccgcaacg ccaggcaaac ggcgaactga ccgcacgcgc tattgctatg ttgcctattt
     1201 tgacgggcaa tgtcggcatc agcggcggaa atagtggcgc gcgtgaatcg acctatacca
     1261 ttaccataga gcgactgccg gtgctggata atccggtcaa aacgtcaatt tcctgcttca
     1321 gttggacaga tgctatcgat catggtccgc aaatgacggc aatccgcgac ggcgtccgcg
     1381 gcaaagataa actggatgtg cccattaagt tcatctggaa ctacgcggga aataccctgg
     1441 ttaatcagca ttctgacatc aacaaaacgc atgaaattct gcaagacgaa tcgaaatgcg
     1501 aaatgattgt ggtcatcgaa aactttatga cctcatcggc aaaatatgcc gacattctgc
     1561 tgccagacct gatgaccgtt gagcaggaag atattattcc taacgactac gccggtaaca
     1621 tgggatatct cattttcctc cagccagtca ccagcgaaaa attcgaacgt aaaccgattt
     1681 actggatcct gagtgaggtc gcgaaacgtc ttggacctga cgtctatcaa aagttcactg
     1741 aaggtcgcac gcaggaacaa tggttacaac atctgtacgc caaaatgctt gccaaagatc
     1801 cggcgttacc gtcttacgac gaactgaaaa aaatgggtat ctataagcgt aaagatccca
     1861 atggtcattt tgtcgcctac aaagcatttc gtgacgaccc cgaggcaaat ccacttaaaa
     1921 caccttccgg taagattgaa atttattcca gcaagctggc ggaaattgcc cgtacctggg
     1981 aactggaaaa agatgaagtg ataagcccac tgccagttta tgcctcaacc tttgaaggct
     2041 gggactcccc tgaacgtagt acctttccac tgcaactgtt tggtttccat tacaaatccc
     2101 gtactcactc gacctacggc aacattgatg tcctgaaggc tgcctgccgt caggaggtgt
     2161 ggatcaaccc tatagacgca cagaaacgtg ggattgccaa cggcgatatg gtgcgggtgt
     2221 ataaccatcg tggcgaagtt cggctaccag ccaaagtgac accacgtatt ctccctggag
     2281 ttagcgctat gggccaggga gcctggcacg aggccaatat gtctggcgat aaaatcgacc
     2341 atggaggctg tgtgaatacg ctaaccactc tgcgcccttc accactggcg aagggaaacc
     2401 cgcagcatac gaatctggtc gagatcgaaa aaatataacc cactacaacc ataaattctg
     2461 gcatgacatt ttgtttgaaa agcaataagt gagtaatgat gaaaatccat accacagagg
     2521 cgctcatgaa ggctgaaatc agccgtagaa gtctgatgaa aacctccgca cttggcagtc
     2581 ttgcgctggc aagcagtgct ttcactctgc cattttccca aatggtccga gcggcagagg
     2641 caccggtaga agagaaagcg gtctggagtt cctgcaccgt taactgcggg agccgctgtc
     2701 tgttacgttt gcatgtgaaa gatgacaccg tgtactgggt ggaatctgat acgacaggtg
     2761 acgacgtcta cggtaatcat caggttcgag cgtgtttacg cgggcgctct atccgccgac
     2821 ggatgaatca tcctgatagg ttgaaatatc ccatgaagcg cgtcggcaag cgcggtgaag
     2881 gtaaatttga acggataagt tgggacgaag ccctggatac catcagtgat aatcttcggc
     2941 ggatcctgaa agattacggc aatgaggctg tacatgtcct gtacggaaca ggtgtagatg
     3001 gcggaaacat caccaactca aacgtcccgt accgtctgat gaactcttgc ggtggttttc
     3061 tcagtcgcta tggcagctac agtaccgcac agatcagtgc cgcaatgagt tatatgttcg
     3121 gtgccaatga tggcaacagc ccggatgata tcgccaatac gaaactggtc gttatgttcg
     3181 gaaataaccc ggcagaaacg cggatgagcg gtggtggtgt cacttactac gtcgagcaag
     3241 cccgcgaacg ttcaaacgca cgcatgatcg tcatcgatcc acgttataac gacacagctg
     3301 ccgggcgtga agatgaatgg ctgcccattc gccctggcac cgatggcgca ctggcctgtg
     3361 cgattgcctg ggtgctgatt actgaaaaca tggtcgatca gccatttctc gacaaatatt
     3421 gtgttggtta cgatgaaaaa acgctgcccg ccaacgcacc acgtaacgcg cattataaag
     3481 cctatattct gggcgaaggg cctgacggca tagctaaaac gccggaatgg gcagcaaaaa
     3541 tcaccagcat tccggcagaa aaaattatcc agttggcacg agagatcggt tcagcaaaac
     3601 ctgcttatat ttgtcagggt tgggggccac aacgacactc caacggcgaa caaacatccc
     3661 gcgctattgc catgctttcc gttctcaccg gtaacgtcgg cataaacggc ggcaactcag
     3721 gcgtacgcga aggtagctgg gatctggggg tagaatggtt cccgatgctc gagaatcctg
     3781 ttaaaacgca gatttccgtc tttacatgga cagatgccat cgaccatggt acggaaatga
     3841 ccgcgacacg tgatggtgtt cgtggaaaag aaaaactgga tgtccccatc aagtttttat
     3901 ggtgctacgc cagtaacaca ttgatcaatc aacatggcga catcaatcac acccatgagg
     3961 tgcttcagga tgacagcaag tgcgagatga ttgttggcat tgaccacttc atgacggcct
     4021 cggctaagta ttgcgatatc ctgttgcccg acctgatgcc aacagagcaa gaagacctta
     4081 tctctcatga atctgcaggg aatatgggct atgtgatcct cgcccaaccc gcaacctcag
     4141 caaaatttga acgcaaaccc atctactgga tgctgagtga agtcgccaaa cgcttaggac
     4201 cggacgttta tcaaaccttt actgaaggtc gcagtcagca tgaatggatc aaatatctcc
     4261 atgcgaaaac gaaggaacgt aaccctgaga tgcccgacta cgaggagatg aaaacgaccg
     4321 ggatctttaa gaaaaaatgc ccggaagaac actacgtcgc tttccgcgca ttccgtgaag
     4381 atccacaggc aaacccgttg aaaacacctt cggggaaaat cgaaatttat tctgaacgac
     4441 tggcgaagat tgcagatacc tgggaattga aaaaagatga aattattcat ccccttcctg
     4501 cgtatacccc aggttttgat ggctgggacg atcccctgcg gaaaacctat ccactgcagt
     4561 taacgggctt ccactacaaa gcgcgtaccc actccagcta cggcaatatt gatgtgttac
     4621 agcaggcctg cccacaagag gtgtggatca accccattga tgctcaggct cgcggtatcc
     4681 gtcatggcga taccgtgcgg gtatttaaca ataatggaga aatgctgatt gccgcaaaag
     4741 tgactccgcg tattctgcct ggcgttaccg ccatcgggca aggtgcgtgg cttaaagcgg
     4801 atatgtttgg tgaccgggtc gatcacggcg gcagtattaa tattctgacc tctcaccgcc
     4861 cttcaccgct ggcaaaggga aacccgtcgc acagcaatct tgtccagatc gaaaaggttt
     4921 aaggagtagc ccatgaccac acaatatgga ttttttatcg attccagccg ctgtactggc
     4981 tgtaaaacct gcgaactggc gtgcaaagat tttaaagttg gttacgtatc gtttaaaatc
     5041 aattagatag cccgtcattt ctgcgctcac acgtcccaac attgaaaaac atgcaaagct
     5101 ttgtaagccg atgcaaagct ttgtgtgtct cacttttgtc ccaataccaa tccgaatcca
     5161 ccctttgtca tcattgagaa atggcaagga agaaaaggat gttactgcac gatttgacaa
     5221 atccgccaga gctatcgcat actgaccgca ctgaacttac aagcggtcat ccgcacccga
     5281 tagctttgcg gcttttttat gcccgtaatg cggcatagtc acatccgtac aaaggtcggg
     5341 tggagaggcg taatacaata cccgtaaggg gaatatgccc agagcttctt gtaaggctca
     5401 gttgacaccc gaccaaccag ctactaactg gttgagctaa ctaaacttac aaggaggtca
     5461 tcatgactag tcaactcatc cccgtattca acggcactat cgccaacgaa accactcttc
     5521 tcgttaatgc ccgtgattta catacttttc ttggggtagg taaacgcttt gcgtcttgga
     5581 ttacagaacg cattgctgaa tatggtttcg ttgaaaatca ggactatatt ttggtttccc
     5641 caaatcggga aatcaaaggt cgaggaggcg atcgccgtag caaagactat cacctcaccc
     5701 tcgacacagc caaagaaacg gcgatggttg agcgtaacga aaaaggccgc cagatccgcc
     5761 gctacttcat cgagtgcgaa aagaaacttc gcaacattca gccagtacaa actgagctgc
     5821 aattcacagc cgaggaaatc atcctcctct gctacatgca actctggatg gaaaaagccc
     5881 aggacctcag caaacacctg tatccgatca tgaaagagct gaactcatca tacacgaaca
     5941 agctgtatga cattgcgttt gagactatct acatggtgac gaagaacaga gatgcgttac
     6001 tgagggaggc tgcacggctt gaccagtcaa gttttgtagt caaacgggcc ataccaatgc
     6061 tgaaaaccct gcgggcaaga caatttgaat tctgaaacct aaggagcttc ggctcctttt
     6121 ttcatgcctg aaggaaagga aaatggcaga tatcatcgac aatgccgctg aaatcgaaga
     6181 attgcagcgc aatctctccc tgcaaaaata caaatccgat agtaatgccc catctgctac
     6241 tcattgttgc gagtgtggcg atccgataga tgagcggcga cgccaggctg ttcgtggatg
     6301 ccgagcttgc gcaagttgcc agcaggatat tgaacttatc aacaaacaga gaggtgtgaa
     6361 gtgaacggag ttagctatca agcactgcgg ctggcggcag aaaatgcaac accaggcgaa
     6421 tggtgtactg atgactatgg ggttattgcc aatgccggac tgaacgccaa ttactacata
     6481 gcatcatgct caggaccaga taatcgcgcc aataagcgat tcatagcagc agccaatccg
     6541 gctaccgtct tggcactgct ggatgaacgg gaaagaaacc agcaatacat caaatcacgc
     6601 gaccaggaga acgaggatat tgcgctaacg gtagggaagc tgcgcgttga gctggaagca
     6661 gcaaaatcaa aactcaacga gcagcgtgaa tattacgagg gtgtaatcgc ggatggaagt
     6721 aagcgcattg cagaactgga gaaaagcgaa gagcaactca ttaatgagcg tgaccatgct
     6781 gagtctgctt tagctgatat gtactttgca gcaaccgggg ataggcctga gtggagtaac
     6841 tgtttcagtt tttcagatgc tgtcgatgcc gtggttgaca gaattgctga tttagaagct
     6901 aaacagccat cgccagtagt gccggaagga ctggttaaag cagtgcgctt ctatgagcag
     6961 gtaaaacgtg agaatccgcc agccgaaacc ggagcatgga aagacgctgt tgactgggtg
     7021 ctcaaagagg cttgtcaggt tgtaaacact ggcatcaaag gagagtgata tggcgctaac
     7081 acaccgcgaa ctctgtcaga ttgcgtacaa gttccttaag cgcaacgggt tcaaggtttg
     7141 ttttcatgac cgctttatag ctgtaaccag taccggagaa cagccagatg ctatgggatt
     7201 cagaaattca gcatcatgcc tgatagaggc gaagtgttct cgtgctgact tgttggcaga
     7261 tagaaaaaag cgttttcgta aaaatccgtc tcttggaatg ggcgactggc gattctttat
     7321 tagtgagccg ggaattattt cagttgagga tttaccagct ggctggggat tacttcacgt
     7381 tgttaacgga agagtgcgga aagtacatgg gtggcccaag ggtaattgct gttggggtaa
     7441 tcctgacgat aagccattta ctggaaataa gcaggttgaa tgcgattaca tgttatctgc
     7501 attaaggcgc atggagttga gagggcacct taatgaaata tatgacggtg taattgttaa
     7561 taagaaagaa ggaaacgcgg catgatcact attaccaaag agcgactgct gacaatcaag
     7621 cagtggcgcg aaacatacgg acctggtagc aacgttgtac tgccagcaga agaagcggaa
     7681 gaactggcac ggattgcact ggcatcgctg gaagcagagc ctgtagcgta atgcattgtt
     7741 gaagatgggg gcatgtgtat tgacgggttc ggtgagtatg tgggtcactc gctacctgat
     7801 ggaacgcacc agctttacac tgcccctcca gcgccagtag tacctgaaga aaaaccaatg
     7861 cctaaccctc ttaaaatgta cgcggtcgat gctgttgccg ctattgcaga ggtgagaggc
     7921 tggaactcct gccgcgcctc catgcttcat ggtgccgaac ctgtaagcca aacttacaag
     7981 ttgaacgagc tgtcgagcaa ctctccggta actccggctc gttggataag ctgtagtaca
     8041 cgaatccccg ctcaagatga ttggatttta atttattcaa agcacggcga gtatatggca
     8101 ggacaggtac aaggggaata cgtggagttg agcgacggca ctttatcgtg gttagggagc
     8161 gccttattct ggatgccgct gccagaaccg ccgaaggagg tacgccaatg agctagcctg
     8221 aagccttctc aaatgttggt attgcaatgg cggtagcgct gatggtgtat tcgatttgcc
     8281 gctggggata aaaacggttt gcggggaaag aagagttaag tagaattgct gcgggtgctt
     8341 gaggctgtct gcctcgggca tgccgccgta aggcagacat agaaaagccc cagttaacat
     8401 ttcgcgtctt gcaggacgct taacattaat ctgaggccaa tttcatacta gacacatgta
     8461 ggttagcctc ttacgtgccg aaaggcaagg agaagcagtc tatgatgcag caaaaggcga
     8521 tgttaatcgc cctgatcgtc atctgtatta ccgttgttgt gacggcactg gtaacgagga
     8581 aagacctctg cgaggtagga atccgaaccg gtcagacgga ggtcgctgtc ttcgtagact
     8641 acgaatctga gaagtaagag acctggcgag ggagtaatct cccgccacct ctgatgtgtc
     8701 aggcatcctc aacgcacccg cgctttacca tactgaaaat gctgtttgaa tgttcatctc
     8761 tgaaagagga ctatgaatga aaaaggtatt gattgcagca cttatttccg gtgtgtcttt
     8821 tggcgttttt gcacagcagg gcggtttcca agggcctgaa gcagagcgtt caacagtagc
     8881 gcaggcaaaa gaactgaagg atgatgcatg ggttatcctt gaagggagca tcgttaaaaa
     8941 agtgggtaat gaacgttatg agtttcgtga caatagcggg acaattgtca cggatattga
     9001 tgacagcgta tgggccgggc agaatgtttc tccgaaagac aaagtaagaa ttgagggtga
     9061 aattgataaa gacctgagca gtgttgaagt ggatgtaaag gcactgaaat tattaaagta
     9121 accgtcccag cttgctaagc ccgtcttact gacgggtttt ctgtttgtta tcacagtatt
     9181 ttcaacaaaa caccgaatct tatccggtgc gctgtaaaaa ccccgtcctt cagggcgtgg
     9241 aggatgtcaa atggcagggg tgaacatcat cccggttaca tcagatcgcc taataacata
     9301 tcaagattat tttgctcata aatgctattc gccattaccg ttgctgcttc agcttaatga
     9361 aacgcataga tccgccatgc tttttcagca aatacgctgc catttgcgca actttttctt
     9421 cattaaacat aaatcggctc tctgtatcac atgaaatgct ctttctgact atatgttaat
     9481 agctccagtt attacaggtc aatgagtgtt tatttcttaa tcagccccgc acttccgtac
     9541 ggggcatgtt ctgttactcc actcccactt ttttgttgta ctcgcggtta aagcgatcca
     9601 cggcaacctt catatcccgc tctaccgatt tcaccatcgc cgcctgctgc gccagactga
     9661 gagaactgtc ggcataaatg gcatcgcgct gtttgcgcaa atccttcagt ctcttccggg
     9721 tatcctgcat aagcccgttc atcgacaatt ttccgttgtt ctcgtcaatg aacgccgttc
     9781 tttctgcacc ggtcagactc ttcagctctg cgtgatactg cgcaacctct gtcatccggt
     9841 cgtacatctt ctgctggtct gcatacggca tcacctcacc cgaaattttc cccaggaagg
     9901 gcacctgctg ttccggtata tcaataccat tcagcgattt caccgccgca tccgtggttt
     9961 tggaaatgaa gcgccctgtc cctccggaga tatagtcaat ccagaatatc agcgattccg
    10021 gtgtgatatc caccgcgccg ggacggtact gactgccacc agagaacgca ttcagccaag
    10081 atgcaaacgc cttgtacgct tctggcgttg aacgtattcc cagctggctg tcaggttttg
    10141 gtgtaccaaa cggtatgttc tcctggtaaa tctgcgcccc catgaagttt tcattcatgg
    10201 caaggttcgc aaacggacgc agaatggtcg gcgctgcatt tttcagcaat gccccggaca
    10261 gtgtttccga cgtctcactg ccgatcgggc tgaatgcccc caggatacca ccgacaacat
    10321 taccggcagc acgggatgcc gtcaggtcac ccgccgccac accttcagcg gtatgcccga
    10381 gcaggaagaa aacgttgtac ccgtaaggca gaggaatact ccagtactct ccggccttgc
    10441 ccccgaacac cgatttcata atgacgaggt tacgctcttt cacatgagac ggcactttgt
    10501 cataccagtt aaccccgtca tcatcctccc ccgcaacact gcggttaagc gagccaagca
    10561 gataacccgc tcccacagct gcaagcgcga ttttctgcgg tacattgaga ttcttccagc
    10621 gaaggcgctc cagtaacggc ccgtcgccat taagatgtcc gagcgttctc accaggtttg
    10681 ccgtcccctg aatgctggcg ttggcaaaca tatacagcga gttcatcagc gctccctgct
    10741 caccacgacg attaaagttc accgtcatgt ttttggcaag agacgccgcc tgctggcgtg
    10801 acaaaccggc atcacgggcg tgtttatagg cagaaagacg cagagcattt tcaaccgccc
    10861 cgttggcatc ctcgacaagg ttaaggaacg aattccatgc accgatactc tggcctttcc
    10921 atcctccctt cgccagcgat acaaggcgat ccatttcctt ctgctggcct tcaaggtcac
    10981 ccatgttaaa ccagccggtt ttacctccgt cctcaacaaa ctctttccac accttctgcc
    11041 actgcgcacc tttgcccgtg aggtttttgc cacgcagact ggcgtatacg gctgacatag
    11101 cagaacggct gtctttcacc acagccagtg cggataagtt atccagccct ttcagtttgc
    11161 cgtcgctcct tccctgctcc gccttcagat tcatcaccgc tgtctgcaca tcacggatga
    11221 agttactgac caggaattct gggttatacg acgtgttcac cgttgccagg aagcggttaa
    11281 ctttccccag cgtacggatt actgcgttac tggtttccgg tcccatattc ttcatcgcac
    11341 gcatcaggcg cggatcatgg agtttgatgt agtacgtttt gccgttcttt ttggtggtga
    11401 agtaccggtc tgccatcatt gccatcgcca ccggacgctc gacaacttcg cgaatggttt
    11461 caccagtttc ctggtccttg cgctctgcaa tcacacgcat ggtatccggt ctgtcatcag
    11521 tgaatacctg ccagtaatcc ttatcgggat tatcctgcac cagcttcagg aaggcgttac
    11581 ccacttcatt tttgcgactg cggatcagcg attcgctcaa gtcctgtatc gcctgagtgg
    11641 acggagactg tgcgcgggat gcacgcccca tggcctgctt actttcacgc ccaccgatgg
    11701 tgaagccctt acctgtacgg ggcagtgaca ccacaccgtc aacatcctgc cctttcaagg
    11761 gaacgtagta acggtaggcg ttctgccagg catccaccac accgctctct tccagtccgg
    11821 cctcacggat aagctcacgg cgacgggcca gcatatcgtc aacaatccct gccagacggt
    11881 catactgtgc ctgtttgccg ctgttacgta cacgctgcat gatttccgcc gcttccgcgt
    11941 tggtcatccc cgaaccgccg tccggcattt tcgggttgat tttcgcgata tgcgcgttac
    12001 gttccggcgc gtgacgggcg tagaggtact catccagatc ggcctgcgca attttgtagt
    12061 ccgccagtaa tttagccagt ggctgaacgt agcgctcctt catcacgttc aggtcgtttt
    12121 ccgccttccc gtggaagagt tcttccgcca tataagcgtt gttactgtcg tctatttttc
    12181 cgccagtttt acggatattc tcctgaacag ctttcagcac ctggaattta tcctgcatct
    12241 ggcgcacaaa acgcgatgca attgtctctt ccggtgtcag actgctggta cgggagtaat
    12301 acggcccctt gcgaatatct tcaggataga gtattttatc tagagagccg cgataagaac
    12361 gctcctcttt gggcaattga agcccactgt actgagggtt atcgcggctt aatttttcct
    12421 tccggtaaag cgtcaaacct gctttatcca ggctattagc tttgttcatc cctccttttg
    12481 ttccatatac agaagccacc ttgttaattt ctattcgttt atctgttgcc ttcatgtgca
    12541 ctgccgacac caccggatca ccatttttat ccacggcatc aagcagcatc acaaccgcat
    12601 ttctttctgt cgcggaacgg taaattgcat ccggatcgtg catcagctca ggaagtctct
    12661 cgataacatc catcggcacc acatgtttca cgccattggt tgccttccgc acagtgtcgc
    12721 gggagataac cagcggcaaa tctggtgcgc caaggtggcg caataccggc ggcgtacgcc
    12781 cgatgtttgc agtcattgca ccagaacgta acgacttcat cattctggca aggtcatcac
    12841 gataacgctc gccctcacct tccggcactt tgaacggatc aggtttacca ctacgggagt
    12901 actgagacga tgcgtccgcg ccatcctcac gcggcgtgta accttcccgc acacgctggc
    12961 ctaacgtacg gatggtctcg cgaacaagtc tgatatcgtt cagttccgtc ggcttcagta
    13021 accccgtacg acgcagtacc cctttgacca gggcaacaac acgctcccat gccgccacga
    13081 atttattcgg ctgtttctcc gccatatgtg ccagaaattc acccgcctgc acttccggtg
    13141 attccttacc ataagacgca tcaaccttac gccaggcttc acggatggtg gcgttatcac
    13201 tgtcacgggt tttcagcacg gtcttaataa tcgtctgata ttccgctggc gtgacaacat
    13261 gctccatggc atggtggata atctcgtgac gcaacttctc gcgtacggtc cgcccgtcag
    13321 ggatgttatc cgccaccagg acaatttctc gtttatccgg acgatagaat gcgtgcaccc
    13381 tgccgtaacc gtcgaacgat tcacccgcca gcgcttcagc ctctttctgt gacttcacca
    13441 cacggacctt caggtcactg tccttaatgc cgctcatcac gccacgggca accgcttcaa
    13501 cctgcgggac cgggctgcct ttggcttccg cactacggtt aacatccgaa atgagattac
    13561 cttcaggtgt gcgggtaacg cccttacggg aataaaacgc aacgcccttg tccgtctcac
    13621 gggttttcag ggtgcggaac aggtgatcga atgcctcacg aataccgcca tccagttccg
    13681 cattcgtcgg ataagcgtag gtgttatctg tgttgtgctc aggtgcctta cggatattga
    13741 ccagataatc attctccacg ccagccatac gcgccttatc ctgaacataa cgctcaaagg
    13801 cacgtgccgc catttcaaca tccgttgacc agtacggttt tgagcgcacc tcatcgagaa
    13861 gcgctgaacg acgcggcatg tcactgtttt taatggcctg aatcacacct ttaaaagcgt
    13921 cgtaaacctc ctgacgtacc ggatattcag catcaacata cctgccgtct ttaaatacgc
    13981 gcctggcacg ctgtgcttcc gtcataaagt cgccacctga cgtaattttc ccgtcagtgg
    14041 aaacgtcata acgaccaaaa taattatcca gagaatggaa ccattcgtgc gccagcgcac
    14101 ccggtccgtt accttttgtc aggttgattg ccgcctcacc cggctcatag tgtgctgccg
    14161 ccttaccctt accacgggca ccaaatgcca ggccaagacg accgttcagg gaaagcgctt
    14221 ttgtcggtac attcagtact tccgcaaggt catgcagcga gtcataagcc cggttcaaat
    14281 cagcctgacg acgcggacct tccacataat taccaaactg cacaccacgg aaaccaaacg
    14341 catcactgaa ctgctccggt gaaacattcc ccttgcggcg ttctggtccg gtacggtcgc
    14401 ggttggtggc gttgcgctgc tcctcacgcg aaatctcccg catctccttc acatgacgaa
    14461 caagctcatc acgatgtgaa tcaatgtact tacgcgcatc actggctgac ttaaagccac
    14521 ctctcacccg cattttgttt ttaccgtaag cgataaaaat atcgccactg cgggtattcc
    14581 ggtaaacgtc aaagcggatt ttgtcatccg gtgacggtgc tgttttttca tcacctttcg
    14641 cctgtgattt ttcctcctgc tctgcaaacc agactttcgc ctttgccagt aattcatccc
    14701 tgctttccga gaaaaagagg ttactcccct tattgtcctt attgcgcagt gaataaagtt
    14761 tctgtggcgg atcgtaacgc ttccctcctg ccgcctgata cacacccgat accacccgat
    14821 aagcagaagc tctgtccatc tgtgagggtg gcagagtgcg taacagttgc caggtgtccg
    14881 cgtaacggga gggcattctg ccttccatcc attctgcgag gcgtttcgcg ctgaccgttc
    14941 cgttcagcat ttccgataca ctgtgtcgta ctttttttat gctctccccc cagcctgccg
    15001 tattgtgttt cgtcttcgcc ggaatatcgc tacgatacag cgctatcatt gccagggtat
    15061 cagcatcggc accttcgttc gccagttttg cgtagtccgg tttcgggaac agtttgctca
    15121 gcggctgcgt ggcataatcc ctgtcttcca gcgttttacc cagtgtttca gcaagctgtg
    15181 cataacggtg tttggctgcc cctttaattt cctcgccaaa gtcttcaatt tttttgcccc
    15241 tgacttcacc tccgcgcacg gtagcttccc tgtcagtaga gacagttgtt tcagattcag
    15301 gtgcgattgt ctcaggctgc gggatgtcac gatttcctcc ggcctgtcgg cgtacttctg
    15361 cctgctgtgc aagctcaggc agtgagtccc gtacagtgcg gggaagtgat tcaggctgag
    15421 gcatacgaac attacgcgca atgtcaggag ccggtagccc ttcacgtacc ggtgctgtcg
    15481 cgttctgcgc atccggtgac ggcagaccac gacgaaccat ttcaccctcg agtgtttccc
    15541 cctggcgacc agcggcgttt tccggtgccg gagcttgtcc tttctggaaa ctctggccct
    15601 taacctcacc ggttgtggta aaacgaccac cacgtcctgc ctgattctca tccggcgtac
    15661 gcgccacctc tcccggtaac ggatatccct gtccgggatg aatatcgccg ggagcgggca
    15721 ggcgtggacg ctcagttaac tcctgtgctg tcggaccggc atcaccttca gccatctgtg
    15781 aacgtacaag ctcctctgcc gtcggcacgt tgccacgggc aaggcgggcc tgtacctccg
    15841 tatcatcccc cgtgaaacca cggaatcgtg ggtcacgcat gaacgcgggc tgctccatcg
    15901 ggtcagcatc agcaatacgc tctccgtcag ccagggtgtt aatggtttcc gttgccacat
    15961 cttcagagaa cgcaggatta tccatttcaa cggcatactg attctcgcct ttcttcacta
    16021 cggacggttt caggccggta gcggcggcat tacggaaaac atcactcccc atggcgcttt
    16081 tctcatccgt gaaataacgg ttgtccgggc gcaccttttc aatgcgttcc gctggtctgg
    16141 acgcaccatt cccgttaatt tcaatgcgga cttcattcgg gtcccggaac cgcaccgtcg
    16201 ggtaaatgct cccgttacca tcctccgcat tttcaggctg tgactgtgct tccggcgtca
    16261 cttcctgttg tgggcgaatg acttctcgta ttgcctgaaa ctgttgttct tcagtctcgg
    16321 tgcgcccctc tttctggctg agtctgcggt attcctcaag cagttcagaa cgcggcttcg
    16381 ccttcagctc attcatcaca gcctgtcgct tcgcctgttc gtccagttca ttcaacaact
    16441 ggctggcagc ttcccggcga tgagctgcgg atgcgtcacc ctctgttgcc atatccgcat
    16501 cagcatactg ctccagaagc tgctcgcgat tcatgccctg catggattca cgctgctgcg
    16561 ccaccggatc aacagattct ggttgcgggg ctgcgtcgtc ctgctgcacg gtttcagcat
    16621 cacgcatggc ggtttcttct gcggcctgac gtctgccacg atatccggca accgcaccgg
    16681 atggagcccc catcgcagca ccaaaggctg caccttcgat cgttgcgtca gccacgccct
    16741 cccacggtga cacatccatt ccggcggtct cacgcaatgc cgtgttttcc tgatagcgtg
    16801 aatagccgcc ctgcgccgca ttaatcgccc cctgttccgt ggcatttctg acaatgccgc
    16861 ttttaacggt cttcgctgtg cctcgtgtca ccagattaaa cagttgtgcg tcacccagtt
    16921 ttgccgccat ggcattcaca gccagcaatt caggatcggt tgccagctgc gcgcgcacct
    16981 catcggcaac acgctctttt gccagatcca ttttctggcg atcagtaagc tgtgcgtgct
    17041 gcgggtcggc gtcaatggac aaaaacgtct gctgaaattt cggtgactgc gccagctcag
    17101 agtaatccgc attaagaaca gcatctgctg ccgccattgc actctgcccc tgtgcactgg
    17161 ctgtggaatg ggtgatcagg cccgcctgga ataaatccgg cattttttta tcgacagctt
    17221 ctgctgccag tgccgtggct ctttccggct gcatccctgc cgcgatgtat tttttctcca
    17281 gcccggcggt cagcattttt cgcagtgtga catcacccac ctttttagcc acaccgccag
    17341 caaccatatc aggtacaagc gcaccaatca ggtttacacc cttcgccacc cagaccgcag
    17401 aatcatcata gccttcggtc atcggcgtat tcagcgcacg aactgcaccc ggcgacatct
    17461 taccggtcag ccattcatca gtggcttcag caccatcact gacagcctta ccggttgcct
    17521 tcagaccttt accgacagta tcagtaaccg cgtttttgcc atcaggcagg gtatcgatga
    17581 cctcatcagc ccccctgctg ccaccggcaa aaatatcctg cacagttgtg acacccggca
    17641 gccccatacg gctgaactca tttaaaatac gcgcccctgt ttttaccggg ctctgaatca
    17701 tcgcatcacc gagtccacgg gccatttccc ctgttccccg gacggactgg gcgaaacctt
    17761 tacccattgt tggcaataca tcgcccaggc tgaacgacgt actgttatcc ttccagcgat
    17821 tcggatcaga gaaaaatgct tcatagctgt cagtttcgcc gggttgctga atgttcaggc
    17881 tgttacgatt ctggttaccg agttgcgcct caggacgctg ttcctctgaa taggccatac
    17941 agactccata aaaaaacccg gcacaatggc cgggcatcag gaaatggtat taatggaata
    18001 tcgtgaatta ctgggcgtaa ttctgtttca gtccacggat aaactgagat gcagatgaag
    18061 cactttcatc tctggcctgt tcccccctgg ccttttgaat acggagaaaa ttcgcatacc
    18121 cgttctccag caatttctga ttctgaggtt ccagcatttc aggctgacgg gccgctacat
    18181 ttctggcaaa tgacagctta tcaggatcat ctcccgccca gttgataacc tgttgctgaa
    18241 gtttttgttg ctgaattttc tgttgatgcg gttgtgaacc agtcgcagca taatattcat
    18301 ccaccgcagc ctgagcatta ccgccattct tgatcgcgtc cgccgctacg ttgctggccc
    18361 ccttctgaag ctctttcaga gaaagtccct gctgcttcgg cataaaataa ccataatttt
    18421 tcgatatctc tgcaaatttg ctgcgatccc tgacctgagc gatagcctta tcaacgggta
    18481 tcgccagcac ggtttgatca tcagggtgcg catgccgtat tctgttacag gtttatgcgc
    18541 ggtggagcca tcgctgtatg tgagatcaag gccaataagt acgtaccctt cctgctgcgc
    18601 cggtacgata ctgccaatcc tggcctcttt tatcgttttt tccccggttg aatcaggcat
    18661 gccaatacgc tgttgtagtt ccggggcaaa cacgccggaa agcacatcga gattttccgg
    18721 ggtattcagc gaagcaatcg ccctgtccgg cttatcatca aagattttct gcaggttgtt
    18781 cacggcctga cccgctttcg cagcatagcc taccgttgac atcatcttaa gcggattggc
    18841 gtccgataat ttaccgaaca gatgagaagc cgcatcatgg tcacccgcat caatcgcttt
    18901 tcccagcgcc gccatcacag gctgatcgcg cgccagcata tcgttatact caagccgtcg
    18961 ctggttgtat ttctgcaact ggagtcgttg ctgttccatc cccagcgacg cattccggta
    19021 attctggttg gcgttaaact ccctttcctg cagtgtgtaa ttgcggtcat caactttggc
    19081 tctgtaatca aagttccgct gatcaacgtc tttattatgt tcaaactgag actgcgcaaa
    19141 ttcaaaatcg cgctcgttat ttttctgttg ctgagcaagc tgtgcttctc gcaaaccaag
    19201 ctccttacga cgggtcattg cctggtcaac agtgctgaat ccggcaagta acccctgtgc
    19261 aaatccgctc attcaccact ccttaaaaca aagcgcctgc aatgccgcca attactgcac
    19321 cagcaacagc gccgacggct gtccccacta ccggtactgc tgaaccaaca gtcgccccga
    19381 tagtggcccc cgtaccaatg cccgtaccga tattctgttt tttctgagct ttctgttgag
    19441 cagccagttg tttatttgat gcctcaatct ctgtacgtcg tctgtctgcg tcacttattc
    19501 cctgtaatgc ctcacgccgc gactgatttg caatatccag taaaccgtat cccatattgc
    19561 ccccttacgc tgccaccagt tgtccgccaa cactcagttt ctgtcttgcc ggtgcagaag
    19621 cccccgtcag aatattcatc tggcgatcct gttcggcttc acggatacca tttttcgcac
    19681 ctgctattgc cagggcatta cgtaacccca gcgtattact gtcgggatta tccgggcggc
    19741 ttaccccgta tctcgccatc tggttatcct gcgccatctg cgctgtacgg agactggaag
    19801 tggcaaggcc tcccacccgt gcaagctgtg gattcatcag actgttgttc tccccaaggt
    19861 cagccagcct tgctacacgg ggtaaatatc tggtgcgcca gtcgtcgtat tgctggcggg
    19921 tcagcgctgc ggatgtctgc cagtccccct gagggcgggc tgctccggta taaccgtatc
    19981 tcgcacgtgt ttcgtattta ccgtactcca taatcacagt ctccagttct gagcctgatg
    20041 ctgaatggca ttagcgccag tgccaggtgt tttagcaccg ccacttcctc cgctaccgcc
    20101 agccttatgc atcgcatatg cacctgccgc acccagacca gcgccaacaa gagaggctcg
    20161 cccctgctgt ttcgtaaacg ccgcctgtgc atccgattta gcttttgcca gactgctgtc
    20221 tgccagagag ttaaaactct gtaacgcatc cgccttctga ccagaaccga gagcagcaac
    20281 atcctgtagc ccggcaacat acttatctgc ctgcgatacc tgcccccgtg tggttgtgtc
    20341 aatctgcccg gttacctgat cactctggtt cgcattcatt accgcgttaa agcgaccact
    20401 ggacgggtca acaccggacc gagtgagatt acttgccagc tccttccgcg cttcgccaaa
    20461 ctgtttttga taccccagat ttgtggtgcc agcaatattg tcgtactgct gctcactgtt
    20521 caggtcatcg accttttcca tgaagttatc ttcagccggg cggaggatat ttttgtaatc
    20581 ctgccaccct ttccaggcca cttcttcctg tgctatttgc gctgctgttg gttttacttt
    20641 ggtatcacca ccacctttac ttccacccat aatggccccc tggataataa aaaaaccctg
    20701 ccggagcagg gtcagaatgt gaattgcaat gttggtttaa acacgatgat gaaaactgtg
    20761 gcagtcagac cgctatcctg aacaccatga agccatcctc atcatccggc attcgctcaa
    20821 agcccagacg ttttcccagc cggataaatc cccgccttgc cgtatgaaat tcagcccaac
    20881 gtccgccagc cagacgggtt aatgtcttca cctccggcag ataacgctca acactgttac
    20941 tccccgtaca cacgcccagc aacaccagaa cataagggat accatcagca ctgagcacag
    21001 aacgcagcac cagaaagcca tcaggtgcct caaaacaaaa cgcctgcttt ttaaagcagg
    21061 cgtctttaac ttcattcata aattcagggt tgcgggaatt tctcaccaca cgctgcatat
    21121 accggagaat tttttcactc acactctcac cacaaaaaag aacaatgtga ctgtgctgtg
    21181 tcttcagcga cagaaggatg acggagaacg cttccgataa ccaacacggc tggcgttctc
    21241 cgcaaaaaat aaattaccag ccgacagaca tacccgcagc aacacctgaa ccgttctggg
    21301 tatcccatga agcattcaaa cgtacagcag aattttcagt ggtgcggaag tgcatacctg
    21361 ctgcgatagc ctgctcatca cggtagttac ttgcagccat accaaaggag tacgttttac
    21421 cctcaacaac aggaatggca gtcatcgcca ttgctcctgc aatacctgca tctgcacgtt
    21481 tacggttttt ctccacctca tcacgtaact gacggaattg ctgattagta ctacgctcca
    21541 gggtactgac ccggttatct gtgtaggtat tagcagactt cagtgtctgt gcatcaccct
    21601 gaatacgggc attctcttct gcaatacgat agcgctctaa ctgaccatag gttgcggcat
    21661 ccgcagcctg aacaccatca cgaagcccac caattacacg attccccaca tttacttcat
    21721 cggggcgagt ggtctgagag ttagccccta cagcaacact gttcgtatgg tttgcaatgg
    21781 cgttgtaacc gacagcagtg gcgttttctc cgcctgctgt tgccccgcca ccaacctgag
    21841 ttttacctgt gcccagagaa gacagacggg cgtgaatatc tgttacatct gcagcagaaa
    21901 gaccagtgtc aatagtggag tctagcgggt ttacagactt cattttaatt tcataaatat
    21961 attcactgat ttcaggaaca tgtcttgaat acgtttttcc atcagcgccc actgtcgtga
    22021 ccacgccttc gccatctgca ctaaagagaa tttgattggt ttttgtgttt acaaaataag
    22081 ct
//
LOCUS       DAZUCE010000069        21852 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZUCE010000069 DAZUCE010000000
VERSION     DAZUCE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.069"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            81..299
                     /gene="slyX"
                     /locus_tag="ACHY03_002180"
     CDS             81..299
                     /gene="slyX"
                     /locus_tag="ACHY03_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFS3905404.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(348..938)
                     /gene="slyD"
                     /locus_tag="ACHY03_002181"
     CDS             complement(348..938)
                     /gene="slyD"
                     /locus_tag="ACHY03_002181"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFS3905405.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(1033..1233)
                     /gene="yheV"
                     /locus_tag="ACHY03_002182"
     CDS             complement(1033..1233)
                     /gene="yheV"
                     /locus_tag="ACHY03_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFS3905406.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(1243..3048)
                     /gene="kefB"
                     /locus_tag="ACHY03_002183"
     CDS             complement(1243..3048)
                     /gene="kefB"
                     /locus_tag="ACHY03_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFS3905407.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(3048..3599)
                     /gene="kefG"
                     /locus_tag="ACHY03_002184"
     CDS             complement(3048..3599)
                     /gene="kefG"
                     /locus_tag="ACHY03_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFS3905408.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            3730..5643
                     /gene="yheS"
                     /locus_tag="ACHY03_002185"
     CDS             3730..5643
                     /gene="yheS"
                     /locus_tag="ACHY03_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3905409.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAINAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            5643..6665
                     /gene="yheT"
                     /locus_tag="ACHY03_002186"
     CDS             5643..6665
                     /gene="yheT"
                     /locus_tag="ACHY03_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS3905410.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            6659..6877
                     /gene="yheU"
                     /locus_tag="ACHY03_002187"
     CDS             6659..6877
                     /gene="yheU"
                     /locus_tag="ACHY03_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFS3905411.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            6931..7800
                     /gene="prkB"
                     /locus_tag="ACHY03_002188"
     CDS             6931..7800
                     /gene="prkB"
                     /locus_tag="ACHY03_002188"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFS3905412.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(7855..8259)
                     /gene="yhfA"
                     /locus_tag="ACHY03_002189"
     CDS             complement(7855..8259)
                     /gene="yhfA"
                     /locus_tag="ACHY03_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFS3905413.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAV
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            8561..9193
                     /gene="crp"
                     /locus_tag="ACHY03_002190"
     CDS             8561..9193
                     /gene="crp"
                     /locus_tag="ACHY03_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFS3905414.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            9244..11334
                     /gene="yhfK"
                     /locus_tag="ACHY03_002191"
     CDS             9244..11334
                     /gene="yhfK"
                     /locus_tag="ACHY03_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFS3905415.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKT"
     gene            complement(11401..12621)
                     /gene="argD"
                     /locus_tag="ACHY03_002192"
                     /gene_synonym="dapC"
     CDS             complement(11401..12621)
                     /gene="argD"
                     /locus_tag="ACHY03_002192"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFS3905416.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRQLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     YQTLLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(12707..13270)
                     /gene="pabA"
                     /locus_tag="ACHY03_002193"
     CDS             complement(12707..13270)
                     /gene="pabA"
                     /locus_tag="ACHY03_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFS3905417.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHHAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(13302..13904)
                     /gene="fic"
                     /locus_tag="ACHY03_002194"
     CDS             complement(13302..13904)
                     /gene="fic"
                     /locus_tag="ACHY03_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFS3905418.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(13894..14061)
                     /gene="yhfG"
                     /locus_tag="ACHY03_002195"
     CDS             complement(13894..14061)
                     /gene="yhfG"
                     /locus_tag="ACHY03_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFS3905419.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            complement(14166..14738)
                     /gene="ppiA"
                     /locus_tag="ACHY03_002196"
                     /gene_synonym="rotA"
     CDS             complement(14166..14738)
                     /gene="ppiA"
                     /locus_tag="ACHY03_002196"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFS3905420.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            15009..16190
                     /gene="tsgA"
                     /locus_tag="ACHY03_002197"
     CDS             15009..16190
                     /gene="tsgA"
                     /locus_tag="ACHY03_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFS3905421.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            16452..18995
                     /gene="nirB"
                     /locus_tag="ACHY03_002198"
     CDS             16452..18995
                     /gene="nirB"
                     /locus_tag="ACHY03_002198"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFS3905422.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            18992..19318
                     /gene="nirD"
                     /locus_tag="ACHY03_002199"
     CDS             18992..19318
                     /gene="nirD"
                     /locus_tag="ACHY03_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFS3905423.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            19444..20250
                     /gene="nirC"
                     /locus_tag="ACHY03_002200"
     CDS             19444..20250
                     /gene="nirC"
                     /locus_tag="ACHY03_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFS3905424.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            20269..21642
                     /gene="cysG"
                     /locus_tag="ACHY03_002201"
     CDS             20269..21642
                     /gene="cysG"
                     /locus_tag="ACHY03_002201"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFS3905425.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         5272 a         5938 c         5602 g         5040 t
ORIGIN      
        1 gatgaccgcg aaatgagtgc tgtctcgggc agcaaagtta agtagaatcc gcggcgacca
       61 ttcgacaaaa gaggtgaatc atgcaggatt tatcattgga agcacgcctg gcagagctgg
      121 agagccgact ggcttttcag gaaatcacca ttgaagaact gaacgtcacg gtgaccgctc
      181 atgaaatgga gatggcgaaa ctgcgcgatc atctgcgtct gctgaccgag aagttaaaag
      241 ccagccagcc gtcgaacatc gcgtcgcagg ctgaagaaac gccaccgcca cattattgag
      301 gcgtaaaaaa agcggggatt ccccgctttt ttgtcacctt ttcggtatta gtggcaaccg
      361 caaccgccgt tgcctttacc gccacagcag ccttcgccac cgtgttcatg accgtgatcg
      421 tggccatgac cgccgcagca accgtcgtgg tcgtgatcgt ggtgatgatc gtgcgcgccg
      481 tgaacgtggc catgagccag ttcttcttcg gtcgcttcgc gaatcgccac aacttcaacg
      541 ttgaatttca ggttctgacc ggccagcatg tggttaccat caaccacgac gtgatcgtct
      601 tcaaccgcag tgatttcaac cggtaccgga ccctggtcgg tttcagccag gaaacgcata
      661 cctacctgca gttcatcaac gcccataaat acgtctttag gaacacgttg caccaggttt
      721 tcgtcgtact gaccgtaagc gtcgttcgcg ccaacagcga catcaaattt gtcgccaact
      781 tcatgacctt ccagcgccgt ttccaggcca gagatcaggg aaccgtgacc atgcaggtag
      841 tccagcggcg cactcaccgg agactcatca accaacacac cgtcttctgt acgtacctga
      901 taggccaggc tgaccaccag gtcttttgct actttcatga tatctcctga gcatgggaag
      961 aatagtggcg cagattgtag cggaattctg cagccgtgta ctcactagct taaaaaaact
     1021 cggcgcatat cgctagtccg gatgaaaaat cccgatcact tgctcatctt tgcgaacgtg
     1081 atcgcgggct tctttgtctg cttctcgcat ctgatgtccg cacttaacgc attcaacaat
     1141 atcaatatta ttttcgcgcc acatcgccat tgaatcctgc gcctgacagg ccgggcattt
     1201 tgcgcccgca ataaaacgtt ttcggattgc catctttacc ctctactcaa attcatccca
     1261 gccgtccagc tggcgtcgtt cttgttgcat ttcacgctgg aaaatctctt ccagttcgcg
     1321 tcgggcttcc ctggcgcgag aaatttgtac ggtatcggca tgcattggga tgagctctcg
     1381 cagcatccgc atatccaggc ggcgaaaatg cagttgcgcc cgctgtgcct gatgcggatg
     1441 catgccaagc gtgaccagcg tcttgcgccc cagctctaac gcactggaga atgtttcacg
     1501 ggaaaactgc gtcacccctg cctgtaataa ctcatgcgct tccacacgtc cgcgcgctcg
     1561 cgcaagaata tgcaaatgcg gaaagtgctg ctggcatatt tccaccagct tcatggtgtc
     1621 ttccggctcg ttacaggtaa tgacgataga ctcagcggcc tctgcacccg cagaacgtaa
     1681 aagatcgacc tgcgtggcat caccgtaata aactttgtag ccatatttgc gcatcaggtt
     1741 aacggcgctg atatcccgct ccagcacggt aatgcgcatt ttatttgcca tcagcaaacg
     1801 accaatcacc tgcccaaaac gcccgaaacc gaccacaatc acctgcggtt tatcatcgtt
     1861 gacccatggt ttttcgtctt cttcctccgg tccgttaaac tggcgggata gccatttatc
     1921 taccagcttc atcagcagcg gcgtggtcat catggaaagc gtcaccgtca ccagcaacag
     1981 cgccatctgg tcgccctgga ataagcgttg tgaagaagcg gtagaaaaga ggacaaaggc
     2041 aaactcaccg ccctgactca acacgccagc aaactgcatc cgctctgagc tacgcacgcc
     2101 atacaatcgc gccagcagat acagcacgag aattttcacc gccaccagca caaccacgct
     2161 tatcactacc cacaacagat gggtataaag caccccgagg ttgagcgaca tgccgacaga
     2221 gataaagaac aaaccgagca gcaagccttt gaagggatcg atagccgttt ccagttcatg
     2281 gcgatattca ctttccgcca gcagcacgcc cgcaataaac gtaccgagcg ccatcgacag
     2341 ccccagcgcg tccataaaca atgcggaacc caataccagc agcagcgtcg cggcggtgaa
     2401 cacttcccgc acgccagaag ctgcaataaa acggaatacc ggacgcaata aatagcgccc
     2461 gccaatcagc atgccgacaa acgccagcac cttcatgccg atcttcatcc agtcgaaatg
     2521 ttcgtctgcc gaacccgcca acaacggcac taacgccagt gccgggatca ccgccagatc
     2581 ctgaaacagc agtaccgaga aacctaactg gccggattcg ctgcgattca tccctttctc
     2641 acgcatcaat tgcaacgcca ttgcagttga agacatcgca aggccaatgc caccgaccac
     2701 cgccgcctgc caggcgaaat ccgtcagcat caataatccc gccagcaacg ctgcgcttaa
     2761 cagcacctgt gccgcgccta cgccaaaaat cgaacgccgc agttgccaaa gcttggaggg
     2821 attcaactca aggccgatga taaacatcag gaacaccacg ccgagttccg aaaagtggag
     2881 gatctcatcg acgtcgctaa taaaccccag cccccacggg ccaattgcaa tccctgccag
     2941 caaatatccc aacacagcgc caatacccag ccgcgatgcc aacggcaccg cagccaccgc
     3001 cgcgaagaga aacagcactc ctgcaagtaa aaaatcggaa ccttccatca gcggcctcct
     3061 ggagacagcg gatttgccag ccagtcaccg taggctctgg catggctcgc cagctcctgt
     3121 gcgctttgcc gtctcgccca gtaaataatg atgggactta accaatgcat ccggcacatg
     3181 cccgccgcca gttcaaaggg gcgcaggaca tcgctcatcg ggtagcgatt caacgcgtca
     3241 taacggtaag cactttccgg ctcgccggtg gtaatcacac tacgccagta ctttcccgcc
     3301 agttggtttc ctcccggccc gctggcaaaa ccacggctta atacccggtc cagccactct
     3361 ttcagtagcg ccgggcagct ataggtataa agaggatgct gaaagacaat cacctcgtgc
     3421 tcgcgcagta atgcctgctc acgggggata tcaataaaaa aatcgggata gtgcgcgtaa
     3481 aggtcgtgca cggtaacatt gctgagctgc gtggccggtt taagcagtac ccggtttgcc
     3541 accgagtcct gagattccgg atgggcatac agcagcaaaa ctttcgctgg ctgagacatc
     3601 atccccctcc cggtatggtt tttgtgtata gtcgctgttt tgggctacca ttgcgcccgg
     3661 tgcggcagct cgcccataca ttacattatc ataatgataa gttaacatag tctgaacata
     3721 cggcacctta tgattgtttt ctcctcgtta caaattcgtc gcggcgtgcg cgtcctgctg
     3781 gataatgcca ccgccaccat caaccccggg cagaaagtcg gcctggtggg taaaaacggc
     3841 tgtggtaaat ctaccctgct ggcattgctg aaaaatgaaa tcagcgccga cggcggcagc
     3901 tacacctttc cgggaagctg gcaactggcg tgggtgaatc aggaaacgcc ggcgttaccg
     3961 caagcggcgc tggaatatgt cattgacggc gaccgtgaat atcgtcaact ggaagcgcag
     4021 ctgcacgacg ccaacgaacg taacgacggg cacgccattg cgaccattca tggcaagctg
     4081 gatgctatta acgcatggag tattcgctcc cgtgccgcca gcctgctgca cggcctcggt
     4141 ttcagcaatg aacaactgga gcgcccggta agtgatttct ctggtggctg gcgtatgcgt
     4201 cttaaccttg cccaggcgct gatttgccgt tcagacttgc tgctgctcga cgaaccgact
     4261 aaccacctcg atctcgatgc cgttatctgg ctggaaaaat ggctgaagag ctatcagggc
     4321 acgctgatcc tgatctctca cgaccgcgac ttcctcgatc cgatcgtcga taaaattatt
     4381 catatcgaac aacaaagcat gttcgagtac accggcaact acagttcgtt tgaagtacag
     4441 cgcgccaccc gtctggcgca gcaacaagcg atgtacgaaa gccagcagga acgcgtagcg
     4501 catctgcaaa gttatatcga ccgtttccgt gccaaagcca ccaaagcgaa gcaggcccag
     4561 agccgcatta agatgctcga gcgtatggag ctgattgccc ccgcgcacgt cgacaacccg
     4621 ttccgcttta gcttccgcgc gccggaaagc ctgccaaatc cgttactgaa gatggaaaag
     4681 gtcagcgcag gctatggcga tcgcattatt ctcgactcga ttaaactgaa tctggtcccc
     4741 ggttcgcgca ttggtctgtt aggccgcaac ggcgcgggta aatcgacatt aatcaaactg
     4801 ttagccggtg aacttgcgcc agtcagtggt gaaataggcc tggcaaaagg gatcaagctc
     4861 ggctacttcg cccagcatca acttgaatac ctgcgcgccg acgaatcgcc tattcaacat
     4921 ctggcacgtt tagcgccgca ggaactggag caaaaactgc gtgactacct cggcggcttt
     4981 ggtttccagg gcgataaagt aaccgaagaa acgcgccgct tctccggtgg ggaaaaagcc
     5041 cgcctggtgc tggcattaat tgtctggcag cggccgaatc tgctgctgct cgacgaaccg
     5101 actaaccacc ttgacctcga catgcgtcag gcactcaccg aagcattaat cgagtttgaa
     5161 ggcgcgctgg ttgtcgtttc gcacgaccgt catttgctgc gttccaccac tgacgacctc
     5221 tacctggttc acgatcgtaa agtcgagccg ttcgacggcg atctggaaga ttatcaacag
     5281 tggttgagcg acgtacaaaa gcaggaaaac cagaccgacg acgcgccaaa agagaacgcg
     5341 aacagcgccc aggcacgtaa agatcagaag cgtcgggaag cggagttgcg tgcgcaaacc
     5401 cagccactgc gtaaagagat tgcccgtctg gaaaaagaga tggagaagct gaacgcgcaa
     5461 ctggcgcagg cagaagagaa actcggcgac agcgaactgt atgaccagag ccgtaaagcg
     5521 gagttgaccg cctgcctgca acagcaagcc agcgccaaat ccggcctgga agagtgcgaa
     5581 atggcatggc tggaagccca ggagcagctt gagcagatgt tgctggaagg ccaaagcaac
     5641 tgatggcgca gataacgacg accgatgcca atgaattcag cagcagtgct gaattcaccc
     5701 ctatgcgcgg ctttagcaat tgtcatctgc aaaccatgct gccgcgtctg tttcgtcgca
     5761 aggtgaaatt caccccgtac tggcagcggc tggagttgcc cgacggcgat tttgtcgatc
     5821 tcgcgtggag tgaagcccct gcacaggcac ggcataaacc gcgtttagtg gtatttcacg
     5881 ggctggaagg cagtctcaat agcccttacg cccacggtct ggttgaggcg gcgcaaaagc
     5941 gcggctggct gggcgtggtg atgcattttc gcggatgcag cggtgaacca aaccgtatgc
     6001 accgcattta ccattcgggc gaaaccgaag acgccagttg gtttttacgc tggctgcaac
     6061 gcgaatttgg tcatgcgcca acggctgccg tcggctattc gctcggcggt aatatgctgg
     6121 cctgtttgct ggcaaaagaa ggcaatgatc tcccggttga tgcggcggtg attgtctctg
     6181 cgccgtttat gctggaggcc tgtagttacc atatggaaaa gggcttttcc cgcgtttatc
     6241 agcgttactt gctgaacctg ttaaaagcca atgccgcacg caagctggct gcctaccccg
     6301 gaacgctgcc gataaatctc gcgcaattaa aatcggtacg tcgcatccgt gaatttgacg
     6361 atctcatcac cgccaggatt cacggctacg ctgacgctat cgactattat cgtcagtgta
     6421 gcgccatgcc gatgctgaac cggatcgcca aaccgacgct gattattcac gctaaagacg
     6481 atccgtttat ggatcatcag gtgatcccga aaccggaaag tctccccccg caggtggagt
     6541 atcaactgac tgaacatggc ggtcatgttg gctttattgg cggtacatta cttcatccgc
     6601 aaatgtggct ggagtcacgc attcctgact ggttaacaac gtatctggag gcgaaatcat
     6661 gttgattccg tggcaagacc tctcccccga aacgctggaa aatttaattg aaagctttgt
     6721 gttacgtgaa ggcaccgatt atggtgaaca tgagcgtaca cttgaacaga aagtcgccga
     6781 cgtcaaacgc cagctacagt gcggagaagc ggtgctggta tggtcagagc tgcacgaaac
     6841 ggtcaatatc atgccgcgca gtcagtttcg cgaataacct gcaaccgtcg gcggacgaga
     6901 taataatgat agctaagtca tggagttact atgtctgcca aacatccggt cattgcggta
     6961 acaggatcca gcggcgcggg gaccaccacc accagcctcg cgtttcgtaa aatattcgcc
     7021 cagttaaatc tgcatgcagc cgaggtggaa ggcgacagtt ttcaccgtta cacccgcccg
     7081 gaaatggaca tggcgatccg caaagcgcgc gacgccgggc ggcatatcag ctacttcggc
     7141 cccgaagcga acgacttcgg cctgctggaa caaaccttca ttgaatacgg tcagagcggc
     7201 aaagggaaat ctcgcaaata tctgcatacc tacgatgaag ccgtaccgtg gaatcaggta
     7261 ccgggaacat tcaccccctg gcaaccttta ccagaaccca ctgatgtact gttttatgaa
     7321 ggtttacacg gcggcgtagt cacgccacag cataacgttg cgcagcatgt ggacttactg
     7381 gtcggcgtgg tgcctatcgt taaccttgag tggattcaaa aactgatccg cgacaccagc
     7441 gagcgcgggc actcacgaga agcagtgatg gactcagtag tgcgttcaat ggaagactat
     7501 atcaactaca tcacaccgca gttttcccgc acccatctta acttccagcg cgttcccacc
     7561 gtcgacactt caaacccgtt cgcggcaaaa ggtatcccgt cgctggatga aagctttgtg
     7621 gtgatccatt ttcgtaatct ggaagggatc gatttcccct ggctgctggc gatgttgcaa
     7681 ggctcattca tttcccacat caatacgtta gtggtgcccg gcggcaaaat ggggctggca
     7741 atggaattaa ttatgctgcc gctggtgcaa cgattgatgg aaggaaagaa aatcgagtaa
     7801 ctctgctatt acgccggata aaatactatc cggcttcaca acgggatagt taaatcacgc
     7861 ggcaaccact tcatacgagt gagtaatatt caccgctttt tccagcatca acgccactga
     7921 acaatatttc tcagcagaga gatcaaccgc acgcgcaacc gctgcgtctt tcaggtcgcg
     7981 accggtgacg ataaaatgca gattaatgtg cgtaaacagg cgtggtgcct cttcgcggcg
     8041 ttcagaggtc aattttactt cacaatcgac cacatcctga cgcccttttt gcaggataga
     8101 taccacatcg atggcgctac agccacccac cgccatcaac accatttcca tcggacttgg
     8161 tgctttatcg cctgagttgc cgtccattaa aatctggtga ccagaggcgg attcgcccag
     8221 aaaagttaac ccttcgaccc acttcactcg cgcttgcatt tttgctactc cactgcgtca
     8281 attttcctga cagagtacgc gtactaacca aatcgcgcaa cggaaggcga cctgggtcat
     8341 gctgaagcga gacaccagga gacacaaagc gaaagctatg ctaaaacagt caggatgcta
     8401 cagtaataca ttgatgtact gcatgtatgc aaaggacgtc acattaccgt gcagtacagt
     8461 tgatagcccc ttcccaggta gcgggaagca tatttcggca atccagagac agcggcgtta
     8521 tctggctctg gagaaagctt ataacagagg ataaccgcgc atggtgcttg gcaaaccgca
     8581 aacagacccg actctcgaat ggttcttgtc tcattgccac attcataagt acccatccaa
     8641 gagcacgctt attcaccagg gtgaaaaagc ggaaacgctg tactacatcg ttaaaggctc
     8701 tgtggcagtg ctgatcaaag acgaagaggg taaagaaatg atcctctcct atctgaatca
     8761 gggtgatttt attggcgaac tgggcctgtt tgaagagggc caggaacgta gcgcatgggt
     8821 acgtgcgaaa accgcctgtg aagtggctga aatttcgtac aaaaaatttc gccaattgat
     8881 tcaggtaaac ccggacattc tgatgcgtct gtctgcacag atggcgcgtc gtctgcaagt
     8941 cacttcagag aaagtgggca acctggcgtt cctcgacgtg acgggccgca ttgcacagac
     9001 tctgctgaat ctggcaaaac aaccagacgc tatgactcac ccggacggta tgcaaatcaa
     9061 aattacccgt caggaaatcg gtcagattgt cggctgttct cgtgaaaccg tgggacgcat
     9121 tctgaagatg cttgaagatc agaacctgat ctccgcacac ggtaaaacca tcgtcgttta
     9181 cggcactcgt taatcccgtc ggagtggcgc gttacctggt agcgcgccat tttgtttccc
     9241 ccgatgtggc gcagactgat ttatcacccc gatatcaact atgcactacg acaaacgctg
     9301 gtgctatgtt tgcccgtggc cgttgggtta atgcttggcg aattacgatt cggtctgctc
     9361 ttctctctcg ttcctgcctg ttgcaatatt gcgggccttg atacgcctca taaacgtttt
     9421 ttcaaacgct taatcattgg tgcgtcgctg tttgccacct gtagcttact gacacagctg
     9481 ctactggcaa aagatgttcc cctgcccttt ttgctgaccg gattaacgct ggtacttggc
     9541 gtcactgctg agttggggcc attgcacgca aaattgcttc ctgcatcgct gctcgccgcc
     9601 atttttaccc tcagtctggc gggatacatg ccggtctggg aaccgttgct catctatgcg
     9661 ttgggcactc tctggtacgg attgtttaac tggttttggt tctggatctg gcgcgaacaa
     9721 ccgctgcgcg agtcactaag tctgctgtac cgtgaactgg cagattattg tgaagccaaa
     9781 tacagcctgc ttacccagca caccgaccct gaaaaagcgc tgccgccgct gctggtgcgc
     9841 cagcaaaaag cggtcgatct aattacccag tgctatcagc aaatgcatat gctttccgcg
     9901 caaaataata ctgactacaa gcggatgctg cgtattttcc aggaggcgct ggatttgcag
     9961 gaacatattt cggtcagttt gcatcagccg gaagaggtgc aaaaactggt cgagcgtagc
    10021 catgcggaag aagttatccg ctggaatgcg caaaccgtcg ccgctcgcct gcgcgtgctg
    10081 gctgatgaca ttctttacca tcgcctgcca acgcgtttta cgatggaaaa gcaaattggc
    10141 gcactggaaa aaatcgcccg ccaacacccg gataatccgg ttgggcaatt ctgctattgg
    10201 catttcagcc gcatcgcccg cgtgctgcgc acccaaaaac cgctctatgc ccgtgactta
    10261 ctggccgata aacagcggcg aatgccgtta cttccggcgc tgaaaagtta tttgtcacta
    10321 aagtctccgg cgctacgcaa tgccggacga ctcagtgtga tgttaagcgt tgccagcttg
    10381 atgggcaccg cgctgcatct gccgaagtcg tactggatcc tgatgacggt attgctggtg
    10441 acgcaaaatg gctatggcgc aacccgtctg aggattgtga atcgttccgt gggaaccgtg
    10501 gtcgggttaa tcattgcggg cgtggcgctg cactttaaaa ttcccgaagg ttacaccctg
    10561 accttgatgc tgattaccac ccttgccagc tacctgatat tgcgcaaaaa ctacggctgg
    10621 gcgacggtcg gttttactat taccgcagtg tataccctgc aactgttgtg gctgaacggt
    10681 gagcagtaca tccttccgcg tcttatcgat accattattg gttgtttaat tgctttcggt
    10741 ggtaccgtct ggctgtggcc gcagtggcag agcgggttat tgcgtaaaaa cgcccatgac
    10801 gctttagaag cctatcagga agcgattcgc ttgattctga gcgaggatcc gcaaccaacg
    10861 ccactggcct ggcagcgaat gcgggtaaat caggcgcata acactctgta taactcattg
    10921 aatcaggcga tgcaggaacc agcgttcaac agccattatc tggcagatat gaaactgtgg
    10981 gtaacgcaca gtcagtttat tgttgaacat attaatgcca tgaccacgct ggcgcgggaa
    11041 caccgggcat tgccacctga actggcacaa gagtatttac agtcttgtga aatcgccatt
    11101 cagcgttgtc agcagcgact ggagtatgac gaaccgggga gttctggcga tgccaatatc
    11161 atggatgcgc cggagatgca gccgcacgaa ggcgcggcag gtacgctgga gcagcattta
    11221 cagcgggtta ttggtcatct gaacaccatg cacaccattt cgtcgatggc atggcgtcag
    11281 cgaccgcatc acgggatttg gctgagtcgc aagttgcggg attcgaagac gtaatacagg
    11341 ctaaaccatc ggatgaggtg ttcacgccgc atccgacatc tttttcctga tgcgtcttat
    11401 ttacgcccca acaactttcg ccaccgcgtg ggcgaaacgt tgcatccctt catcgatatc
    11461 cgcatcttcc accaccagcg acggtgcaaa acgcatcaca tccggtccgg cattcagcac
    11521 cattacgcca gcctctgcgc ccgcatacag gaaatcacgc gcccgacctt tgtactgtgg
    11581 cttaagctcc gcgccaatca acagccccat accgcgaata tcgctaaaca catcgtactg
    11641 ctgatcgatc ttctgcaaat ggtcaacaaa acgctggcgt ttcgcctgaa tgccttccag
    11701 cacttcaggt gtattgatga tatcaaacgc cgcccccgct actgcacagg ccagaggatt
    11761 accgccgtag gtggaaccgt gagaaccagg atgaaacgca gaagcaattt ccgccgtggt
    11821 cagcatggcg ctaatcggga agccgccgcc taacgctttc gcagaagtca gaatatccgg
    11881 cgtcacgccg tagtgcatgt aagcaaacaa atcgccagtc cgccccatcc cgcactgcac
    11941 ttcatcaaac accaataatg tctgatattg atcgcacagc tcgcgcaagc cctgcaaaaa
    12001 ctctggcgtc gctgccgtca caccgccctc gccctggatc ggctcaacca ccaccgcaca
    12061 ggtgtgatca tccatcaccg ctttcactgc gtggagatcg ttaaagggaa cgtggatgat
    12121 gtctgccggt ttcggcccaa agccgtcgga atattttggc tgcccaccca ccgagacagt
    12181 aaacagcgag cgaccatgaa aagcgttatg gaaggcaata attttggttt tgaacgggct
    12241 atgacgcaca caggcgtaat gacgtgccag tttaaaggcg gtttcgttag cttccgtgcc
    12301 agagttcata aacaccacgc gttcggcaaa cgtcgcctca atcagttggc gcccaagacg
    12361 cagcgccggt tcattggtga aaacgttact gatatgccac agagtttcgc cctgggtttt
    12421 taacgcgttc accagcgcag gatggcaatg gcccaacgcc gtaactgcaa tgccacccgc
    12481 gaaatcgaca tactccttgc cttgctgatc ccagattcgg ctgccctgac cttttaccgg
    12541 aatgaactct gccggagcat aaatcggcag gatcacttca tcgaaagtcg cgcgtgtaat
    12601 tgctgtttgt tcaattgcca tctcatgatc accctgttac gcataaataa atgtgaaatt
    12661 ataatcacaa aatatgcata aaaaatcatt gaatggcaat cagaaatcag cgatgcagga
    12721 aattagccag cagttgatgt ccttgttcgc taagaatact ttctggatgg aactgcacac
    12781 cttccagatc ccactggcga tggcgaatcc ccataatctc gcgggtttcg ctccaggccg
    12841 tcacttcaaa gcacgctggt aacgagtcag gttccactac cagcgaatga tagcgtgtca
    12901 cggtaagtgg atttgccagc ccccgaaata cgccctcacc gttatgtgta atcggcgagg
    12961 ttttgccgtg catgaccttt gcggcgcgca caactttacc gccaaatgcc tgcgccattg
    13021 cctgatgacc gaggcagacg ccaagaatcg gcaagcgccc ggcatggtgg cgaataacgt
    13081 caagggagat cccggcttca tctggcgtac aggggccagg tgagatgaca attttttgtg
    13141 gtttaagggc gtcgatatcc gccagcgtca acgcatcgtt gcgcttaacc agcacatccg
    13201 cccccagttc acaaaagtac tggtagaggt tccaggtaaa agaatcgtag ttatctataa
    13261 gcaggatcat ggcggctccg atacgaaaga accgcgctat tttactcaga ttccccggct
    13321 tcgcttacca ctttgctaaa tatcatctgc agtgcggtga gatcccccat tgccccactc
    13381 tgatttgcct gattccaggc ctctttttca ataccctgcc agctcagttg atatccggca
    13441 tgaatcgcca gttgctcgaa gaagatccgc tgtgccagac cacttcccac ccggaaggga
    13501 tgcagcacgt tgatttcaca atagtaatgc gccagccgct cgacgaactt cgctttctcc
    13561 aggccaacca gataaccttc ttcctccaga tcctgcatca gggcattgcc ctctttttcg
    13621 atataagcaa agtggcagaa cggcgtatcg ccctgataaa tatcaacttc acgcagttgc
    13681 cctgcccagt cgaaaatatc ctgatacagc tggcgatgga tagtgcgcaa atgcggtaaa
    13741 ccgcgcacca gcggaccaag ctcaatggtc gcagcacgca gcgccgtcat ttcgtaagcg
    13801 gcctgttcca gccgttgttg ctggcggatg ttcagccggt tacgcatgat atcaaggcca
    13861 ggataaagat acggatcgcg cccttcgccg aatttatcgc tcatagtgac tcctcagctc
    13921 ttcaaggcgc gctaaagcct ctgcggcagt aagagtgact aaaggcatct cgacgccttc
    13981 aagacggcga ctggcctgaa aattacgatt acgctgaagc tcccagagac gggacttttg
    14041 cttatcggtg agtttcttca cttgatgcct ccctgaacgt gtccatttgc cacaagtata
    14101 agcggcaaat ccgggttacg ccgggagagc agaagcgcgg gcaagattgc ccgcgcgaga
    14161 aatcattacg gcaggacttt agcggaaagg ataactaccg gttttgacgg cacattctgg
    14221 tacggaccaa cgtcatgagt cggcacctgg gaaatcttat cggcaacgtc catgcctttc
    14281 accactttac caaataccgc gtaaccgaaa tcacgctgac catggtcaag gaaggcgtta
    14341 tcggcaacgt tgataaagaa ctggctggtg gcgctgtctt tgtcagcggt acgcgccatc
    14401 gcgatggtgc cacgcgtgtt gcgtaggccg ttatcggctt catttttgat tggcgggttt
    14461 ggttttttct gctgcatctg ctcggtgaaa ccaccgccct gaatcataaa gccaggaatg
    14521 acgcggtgaa aggtagtgtt gttataaaaa ccgctgttca cataatcgac aaagttctgc
    14581 acagacactg gcgctttttg tttatccagc tccagttcga tgttaccagc tgaggttgtc
    14641 aacaatacgt gcgggtcccc tttcgctgcc attgctgcgg gagaaagagc agaaagagcg
    14701 aaaacagcag ccatcgccgc cagggtcgat ttgaacatga gatttcctta acaaacagag
    14761 cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa gaacaaggcc atccctttac
    14821 ctaattttac gtatctgaaa tatatgtaac ctgacgatca ctttttattc cgtgatcaaa
    14881 atcacctctt aaaatgcaat ttagcaaccg attgcaataa aacatttaaa cagatcacaa
    14941 aatcacctaa aatcgcccgc tcgcggcata tcccgctatg ccattttatt tttgaacgca
    15001 ggccaatcat gactaacagc aatcgcatca agctcacatg gattagcttt ctctcctacg
    15061 cactgactgg tgcgttggtt attgtcaccg ggatggtgat gggaaatatc gccgattatt
    15121 tcaatctgcc tgtttccagt atgagtaaca ccttcacctt cctcaacgcc ggcattttaa
    15181 tctctatctt cctcaacgcc tggctgatgg aaatcgtccc gttgaaaacg cagttacgtt
    15241 ttggctttct cctgatggtg ctggcggttg ccggtttgat gttcagccac agcctggcgc
    15301 tgttctcggc ggcgatgttc attctcgggg tggtcagcgg catcaccatg tcgattggta
    15361 cattcctggt aacacaaatg tatgaagggc gtcagcgcgg ttcccgcctg ttatttaccg
    15421 actccttctt cagtatggct gggatgattt tcccaatgat cgccgcgttt ctactggcgc
    15481 gcagcattga gtggtactgg gtttatgcct gcatcgggct ggtgtatgtc gctattttta
    15541 ttctgacttt cggctgtgag ttcccggcgc tgggcaaaca tgcgccaaaa acggatgctc
    15601 cggtagcgaa agaaaagtgg gggatcggcg tactgtttct ctctgttgcg gcgctgtgct
    15661 acatcctcgg tcagttaggt tttatctcct gggtgcctga gtatgccaaa ggcctgggca
    15721 tgagcctgaa cgacgcgggc acgctggtga gtaacttctg gatgtcatac atggtcggca
    15781 tgtgggcgtt cagctttatt cttcgcttct ttgatttgca acgcattctg accgtactgg
    15841 ctggtctggc tgcgattctg atgtacgtct ttaacaccgg aacaccggca catatggcgt
    15901 ggtcaattct cgccctgggc ttcttctcca gcgcgatcta taccaccatc atcaccctgg
    15961 gttcacagca gaccaaagta ccgtcgccaa aactggttaa ctttgtcctg acctgcggga
    16021 ccatcggtac tatgttgacc tttgtggtta ccggcccgat tgttgaacat agcggtccgc
    16081 aggcggcact gcttacagca aacggtctgt acgctgtcgt ctttgtgatg tgcttcctgt
    16141 taggttttgt cagccgtcac cgtcagcata acaccctgac ctctcattaa ttgctcatgc
    16201 cggacggcac tatcgtcgtc cggccttttc ctctcttacc ccgctacgtg catctatttc
    16261 tataaacccg ttcattttgt ctattttttg cacaaacatg aaatgtcaga caattccgtg
    16321 acttaagaaa atttatacaa atcagcaata taccccttaa ggagtatata aaggtgaatt
    16381 tgatttacat caataagcgg ggttgctgaa tcgttaaggt aggcggtaat agaaaagaaa
    16441 tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg taacggtatg gtcggccatc
    16501 gctttatcga agatcttctt gataaatctg atgcggccaa ctttgatatt accgttttct
    16561 gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc gtcttacttc tctcaccaca
    16621 ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga gaaacacggc atcaaagttc
    16681 tggtcggcga acgtgctatc accatcaacc gtcaggagaa ggtgattcac tccagcgccg
    16741 ggcgcaccgt tttttatgac aagctgatca tggcgaccgg ttcctacccg tggatcccgc
    16801 caatcaaagg ttctgatact caggactgct ttgtctatcg cactattgaa gacctcaacg
    16861 ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt tgttggtggc ggcctgttag
    16921 gtctggaagc cgctggcgcg ctgaaaaact taggtattga aacccacgtt atcgaatttg
    16981 cccctatgct gatggcagaa cagcttgatc agatgggcgg cgagcagctg cgtcgcaaaa
    17041 tcgagagcat gggcgtgcgc gttcacacca gcaaaaacac ccttgagatt gtgcaggaag
    17101 gtgttgaagc gcgtaaaacc atgcgttttg ccgacggcag cgaactggaa gtcgacttta
    17161 tcgtcttctc taccggtatc cgtccgcgcg ataagctggc aacccagtgt ggtctggacg
    17221 ttgctccgcg tgggggtatt gtcattaatg attcctgcca gacttccgat ccggatatct
    17281 acgccatcgg tgaatgcgca agctggaaca accgtgtatt tggtctggta gcaccaggct
    17341 acaaaatggc gcaggtcgcc gttgaccata ttctcggtag cgaaaacgcc tttgaaggtg
    17401 ctgaccttag cgccaagctg aaactgctgg gcgtagacgt aggcggtatt ggtgatgcgc
    17461 acggtcgcac accaggcgca cgtagctacg tttacctcga cgaaagcaaa gagatctaca
    17521 aacgccttat tgtcagcgaa gacaacaaaa ccctgctcgg tgcggtactg gtgggcgata
    17581 ccagcgacta cggcaacctg ctgcaactgg tgctgaatgc tatcgagctg ccggaaaacc
    17641 cggattctct gattctgccg gcgcactcgg gtagcggtaa gccgtctatc ggtgttgata
    17701 aactgccgga cagcgcgcaa atctgctcct gcttcgacgt caccaaaggt gatctgattg
    17761 ctgccatcaa caaaggctgt cacaccgttg cggcgctgaa agccgaaacc aaagcgggta
    17821 ctggctgcgg tggctgtatc ccgctggtca ctcaggtact gaacgcggaa ctggcgaaac
    17881 agggcatcga agttaacaac aacctgtgcg aacactttgc ttattcgcgt caggaactgt
    17941 tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga actgctggcg aaacacggca
    18001 aaggttacgg ttgtgaagtt tgtaaaccga ccgtcggttc gctgctggcc tcctgctgga
    18061 acgaatacat tctgaagccg gaacatactc cgctgcagga ttctaacgat aacttcctcg
    18121 ctaacatcca gaaagacggc acctactcgg tgatcccgcg ctctccgggc ggtgaaatca
    18181 ccccggaagg actgatggca gtgggtcgta tcgcgcgtga atttaatctc tacaccaaaa
    18241 tcaccggctc tcagcgcctg gcgatgtttg gcgcacagaa agacgatctg ccggaaatct
    18301 ggcgtcagct gattgaagcc ggtttcgaaa ccggtcatgc ctatgcgaaa gcactgcgta
    18361 tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg cgtgggcgac agcgtgggcc
    18421 tcggcgtgga actggaaaac cgctacaaag gcatccgtac gccgcacaaa atgaagttcg
    18481 gtgtctccgg ctgtacccgt gaatgttcag aagcccaggg taaagacgtg ggtattatcg
    18541 ccaccgaaaa aggctggaac ctgtatgttt gcggtaacgg cggcatgaaa ccgcgtcatg
    18601 cggatctgct ggcggcggat atcgatcgcg aaacgctgat caaatatctc gaccgcttca
    18661 tgatgttcta catccgtact gccgacaaac tgactcgtac cgcgccgtgg ttagaaaacc
    18721 tcgaaggcgg catcgattac ctgaaagcgg tgatcattga cgacaaactg gggctgaacg
    18781 cacatctgga agaagagatg gcgcgcctgc gtgaagcggt agtgtgtgag tggactgaaa
    18841 cggtcaatac gccgtctgcg cagactcgct tcaaacactt catcaacagc gacaagcgtg
    18901 acccgaacgt gcagatggtg ccagagcgcg aacagcaccg tccggcaacg ccgtatgaac
    18961 gtatcccagt aactctggtg gaggacaacg catgagccag tggaaagaca tctgcaaaat
    19021 cgatgacatc ctgcctgaaa ccggcgtctg cgcgctgtta ggtgacgagc aggtcgcgat
    19081 tttccgcccg tatcacagcg atcaggtgtt tgcgatcagc aacatcgacc cgttcttcga
    19141 gtccagcgtg ctgtcacgcg ggctgattgc ggagcaccag ggcgagctgt gggtcgccag
    19201 cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg tgcatggaag acgaacagtt
    19261 ttccgtcaaa cattacgaag cgcgagtgaa agacggcgtg gtgcagctgc gcggttaatg
    19321 ttttaacggg aggcgcaatg cctccccttt ttgcatggtc ctgtaataat cttcggtata
    19381 ttgcaggaca ttttttaaac tttttgtttt attttttgtt tttatttttt aaaggataat
    19441 caaatgttta cagacactat taataagtgt gcggctaacg ctgcgcgcat tgcacgcctg
    19501 tcggcaaata acccgctcgg cttttgggtc agctccgcca tggcgggcgc gtatgtgggt
    19561 cttgggatca tcctgatttt tacgctcggt aatttgctcg atccatccgt acgccctctg
    19621 gtgatgggcg cgacctttgg tatcgcctta acgctggtga ttatcgccgg ttctgaactg
    19681 ttcaccggac acaccatgtt cctcaccttt ggggtgaaag cgggcaccat cagccacggg
    19741 caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc tggtcggttc cgtcttcgtt
    19801 gccatgctct atagctgggg tggtggtagc ctgctgccgg tagataccag catcgttcac
    19861 tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac tgttcttcaa aggtgcattg
    19921 tgtaactggc tggtttgtct ggcaatctgg atggcgctgc gcactgaagg ggcggcgaaa
    19981 tttatcgcaa tctggtggtg tctgctggca tttatcgcgt ccggctacga gcactccatc
    20041 gctaacatga cgctgttcgc gctctcctgg ttcggcaacc acagcgaagc ctacacgctg
    20101 gcgggtattg gtcataacct gctgtgggtg acgctgggta atactttatc aggtgccgta
    20161 ttcatgggat tgggttattg gtatgctacg ccgaaagcga atcgtccggt tgcggacaaa
    20221 tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg gtttttacgt ggatcatttg
    20281 cctatatttt gccaattacg cgatcgcgac tgtctgattg tcggcggtgg tgatgtcgcg
    20341 gaacgcaaag caaggttgct gttagacgca ggcgctcgct taacggtgaa tgcattagcg
    20401 tttattccac agttcaccgc atgggcagat gcaggcatgt taaccctcgt cgaagggcca
    20461 tttgatgaaa gccttctcga cacctgctgg ctggcgattg cagcgacgga tgatgacgcg
    20521 cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca tcttctgtaa cgtggtcgat
    20581 gcgccgaaag ctgccagctt cattatgccg tcgattattg accgctcacc gctgatggtg
    20641 gcggtttcct ctggcggtac ttctccggtt ctggcgcgcc tgttgcgcga aaaactcgaa
    20701 tcactgctgc cgctgcatct gggccaggta gcgaaatacg ccgggcagtt acgcggtcgg
    20761 gtaaaacaac agttcgccac gatgggtgaa cgccgccgct tctgggaaaa actgttcgtt
    20821 aacgaccgtc tggcgcagtc gctggcgaac aacgatcaga aagccattac tgaaaccacc
    20881 gaacagttaa tcaacgaacc gctcgatcat cgtggtgaag tagtgctggt tggcgcaggt
    20941 ccaggcgatg ctggtcttct gacgctgaaa ggtctgcaac aaattcagca ggcagatgtg
    21001 gtggtctacg accgtctggt ttctgacgat attatgaatc tggtacgccg cgatgctgat
    21061 cgcgttttcg tcggcaaacg cgcgggatac cactgcgtac cgcaggaaga gattaaccag
    21121 atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc ggctgaaagg tggcgatccg
    21181 tttatttttg gccgtggcgg cgaagagctg gaaacactgt gcaacgcggg tattccgttc
    21241 tcggtggttc cgggtattac cgcagcttct ggttgctctg cctattcggg tattccgctc
    21301 acgcatcgcg attatgccca gagcgtgcgc ttaattaccg gacacttaaa aaccggtggc
    21361 gagctggact gggaaaacct ggcggcagaa aaacagactc tggtgttcta tatggggctg
    21421 aatcaggctg cgactattca gcaaaagctg attgaacacg gtatgcctgg cgaaatgccg
    21481 gtagcaattg tcgaaaacgg aacagcagtc acgcagcgcg tgattgacgg tacgctcaca
    21541 caactgggcg aactggcgca gcaaatgaac agtccatcgc taattattat tggtcgggtt
    21601 gttggcctgc gcgataaact aaactggttc tccaaccatt aatttaatca gggccagaga
    21661 attctggcct ttttcgttta caactctctt tatatattca gcagataaaa atataactca
    21721 ttaacaaaaa tcatttgttt ttgccccgtt atattcaccg ttacagcgac ttacaaaatt
    21781 aagtcattca ctcactgacg ccgattaaat attcctgtat cgttcttaat gagcagtcta
    21841 cttatattca ta
//
LOCUS       DAZUCE010000070        21824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZUCE010000070 DAZUCE010000000
VERSION     DAZUCE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.070"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..440)
                     /gene="ttdR"
                     /locus_tag="ACHY03_002202"
                     /gene_synonym="dan"
     CDS             complement(<1..440)
                     /gene="ttdR"
                     /locus_tag="ACHY03_002202"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFS3905426.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLD"
     gene            647..1558
                     /gene="ttdA"
                     /locus_tag="ACHY03_002203"
     CDS             647..1558
                     /gene="ttdA"
                     /locus_tag="ACHY03_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFS3905427.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1555..2160
                     /gene="ttdB"
                     /locus_tag="ACHY03_002204"
     CDS             1555..2160
                     /gene="ttdB"
                     /locus_tag="ACHY03_002204"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFS3905428.1"
                     /translation="MKKILTTPIKAEDLQDIHVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            2208..3671
                     /gene="ttdT"
                     /locus_tag="ACHY03_002205"
     CDS             2208..3671
                     /gene="ttdT"
                     /locus_tag="ACHY03_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFS3905429.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLGWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3714..4727)
                     /gene="tsaD"
                     /locus_tag="ACHY03_002206"
     CDS             complement(3714..4727)
                     /gene="tsaD"
                     /locus_tag="ACHY03_002206"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFS3905430.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4965..5180
                     /gene="rpsU"
                     /locus_tag="ACHY03_002207"
     CDS             4965..5180
                     /gene="rpsU"
                     /locus_tag="ACHY03_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFS3905431.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            5291..7036
                     /gene="dnaG"
                     /locus_tag="ACHY03_002208"
     CDS             5291..7036
                     /gene="dnaG"
                     /locus_tag="ACHY03_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFS3905432.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            7231..9072
                     /gene="rpoD"
                     /locus_tag="ACHY03_002209"
     CDS             7231..9072
                     /gene="rpoD"
                     /locus_tag="ACHY03_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFS3905433.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(9151..9657)
                     /gene="mug"
                     /locus_tag="ACHY03_002210"
     CDS             complement(9151..9657)
                     /gene="mug"
                     /locus_tag="ACHY03_002210"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFS3905434.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            9782..9857
                     /locus_tag="ACHY03_002211"
     tRNA            9782..9857
                     /locus_tag="ACHY03_002211"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9815..9817,aa:Ile,seq:cat)
     gene            complement(9910..10674)
                     /gene="nfeF"
                     /locus_tag="ACHY03_002212"
                     /gene_synonym="yqjH"
     CDS             complement(9910..10674)
                     /gene="nfeF"
                     /locus_tag="ACHY03_002212"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFS3905435.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVEHISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEDGVASSWAMQAQPDDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFETEQFDPQRVRAAAYWHAK"
     gene            10962..11585
                     /gene="nfeR"
                     /locus_tag="ACHY03_002213"
                     /gene_synonym="yqjI"
     CDS             10962..11585
                     /gene="nfeR"
                     /locus_tag="ACHY03_002213"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFS3905436.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(11692..13212)
                     /gene="aer"
                     /locus_tag="ACHY03_002214"
     CDS             complement(11692..13212)
                     /gene="aer"
                     /locus_tag="ACHY03_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFS3905437.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            13630..15009
                     /gene="ygjG"
                     /locus_tag="ACHY03_002215"
     CDS             13630..15009
                     /gene="ygjG"
                     /locus_tag="ACHY03_002215"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFS3905438.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(15051..15383)
                     /locus_tag="ACHY03_002216"
     CDS             complement(15051..15383)
                     /locus_tag="ACHY03_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFS3905439.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            15602..16585
                     /gene="ebgR"
                     /locus_tag="ACHY03_002217"
     CDS             15602..16585
                     /gene="ebgR"
                     /locus_tag="ACHY03_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFS3905440.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            16769..19861
                     /gene="ebgA"
                     /locus_tag="ACHY03_002218"
     CDS             16769..19861
                     /gene="ebgA"
                     /locus_tag="ACHY03_002218"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFS3905441.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVDDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVFIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            19858..20307
                     /locus_tag="ACHY03_002219"
     CDS             19858..20307
                     /locus_tag="ACHY03_002219"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFS3905442.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            20370..21803
                     /locus_tag="ACHY03_002220"
     CDS             20370..21803
                     /locus_tag="ACHY03_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3905443.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFSVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAGERHIEATPASNVD"
BASE COUNT         5239 a         5829 c         5647 g         5109 t
ORIGIN      
        1 atatccagat caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc
       61 acctgtaact caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc
      121 ccaaaaccaa aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg
      181 acgtcatcga ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt
      241 tggccacttt cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc
      301 gtattttcaa ggatttggat ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc
      361 gctgcagcac taaaactccc gctgtggacg atttctacca gtacctgcaa atctttggct
      421 aaaggccagc tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg
      481 caaaacattg ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac
      541 gaactgttaa ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt
      601 ttgtggcggt gaatttttcc agacaaatac agaaactgga gttgccatga tgagcgaaag
      661 taataagcaa caggcagtga ataagttgac ggagattgtc gctaacttta ccgccatgat
      721 ttctacccga atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac
      781 gtcgtcgatg gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga
      841 cctgaatcgt cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc
      901 ccgcttccca ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac
      961 cgtcaaagcg ccactacgtc acaatgcggt agaaattttt gacgaagtga acacaggcaa
     1021 aaataccggc agtggcgtac cgtgggtcac ctgggatatc gtccccgacg gtgacgatgc
     1081 ggaaatcgaa gtttacatgg caggcggcgg ctgcacgcta ccaggccgct cgaaagtgtt
     1141 aatgccgtca gaaggctacg aaggcgtggt gaaatttgtc ttcgaaaata tctccaccct
     1201 cgccgtaaac gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac
     1261 cgccgccgta ctctcgcgta aagccatttt gcgcccgatt ggcagccgcc accccaatcc
     1321 aaaagcggca gagctggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc
     1381 acaagggctg actggcaaca gttcagtgat gggcgtgcat atcgaatctg ccgcccgcca
     1441 tccgtcaacc atcggcgttg ctgtttctac cggctgctgg gcgcatcgtc gcggcacact
     1501 gctggttcat gccgatctca cctttgaaaa tctgtctcac acccggagcg cgttatgaaa
     1561 aagatcctga caaccccgat caaagctgaa gatctgcaag atattcacgt cggcgatgtg
     1621 atctatctga ccggtacgct ggtgacctgc cgtgacgttt gtcaccgccg attgatcgaa
     1681 ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga ttttccacgc tggccccatc
     1741 gtgcgcaaaa acggcgacaa atgggagatg gtttccgtcg gtccgacaac cagtatgcgt
     1801 atggaaagtt ttgaacgtga atttatcgag cagaccggcg tgaaactggt ggtcggcaaa
     1861 ggcggaatgg ggccgctgac cgaagaaggc tgtcagaaat tcaaggcgct acatgtgatt
     1921 ttcccggcag gctgcgcggt gctggcggca acccaggtgg aagagattga agaggtgcac
     1981 tggacagaac tcgggatgcc ggagtcactg tgggtctgcc gggtcaaaga gttcggcccg
     2041 ctgattgtct ctattgatac ccacggcaac aacctgatag ccgaaaacaa aaagctgttc
     2101 gccgaacgcc gcgatcccat cgtggaagag atctgcgagc acgtccatta catcaaataa
     2161 ccctcccgga gaggctcgcc cctctccttt ttcgcaggca taacacgatg aaaccttcca
     2221 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
     2281 ttcccgcagg gctggagagt catacctggc tctactttgc tgtttttact ggcgtgatcg
     2341 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
     2401 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
     2461 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
     2521 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctg gggcgacgta
     2581 tcgcgctgat tctggtgaaa aagatggggc atcgcacgct gtttcttggc tatgcggtga
     2641 tgttctccga gctgatcctc gcacctgtca caccgtccaa ctcggcgcgt ggtgcgggga
     2701 ttatctatcc catcatccgt aacctgccgc cgctctatca atcacaacca aacgacagca
     2761 gttcgcgcag tattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
     2821 gcgccatttt cctgacggcg atggctccta acttgctgtt aattggactg atgaaaagcg
     2881 catctcacgc cacgctgggt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
     2941 tactggtcct gctggttccc tggctggctt acgtgctgta cccgcctgta ctgaagtcag
     3001 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
     3061 gtgaaaaacg gatgctgggg ctgatggtag gcgcgctggt gctgtggatt ttcggcggtg
     3121 attatatcga tgccgcgatg gtcggttaca gcgtggtggc gctgatgctg cttctgcgca
     3181 ttatcagttg ggacgacatt gtcagtaata aagccgcgtg gaacgttttc ttctggctgg
     3241 cctcgcttat caccctcgcg accggactca acaacaccgg ttttattagc tggtttggca
     3301 aactgttagc aggcagctta agcggttatt cgccgacgat ggtgatggtg gcgttgattg
     3361 tggtgtttta tctgctgcgc tacttttttg ccagcgccac ggcgtatacc tccgctctcg
     3421 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
     3481 tgatggttgg tgcggcaatt ggtctgggga gcattcttac accatacgcc accggcccca
     3541 gtccgattta ctacggcagt ggttatctgc caacggcgga ttactggcga ctgggggcga
     3601 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
     3661 tgttgcttta agtaaggacg gtgccggaag taactctccg gcacccgata gttttacgca
     3721 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccaagatc cgccgtcgcg
     3781 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaat
     3841 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
     3901 cgtaacgtgc ggttagcact cacgccgccg gccattacca gtcgcttaaa gcccgtctga
     3961 tccagcgcac gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
     4021 gcgatgtcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatggtatt tgccgcgaag
     4081 gttttcaggc cggagaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
     4141 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
     4201 tccagcccca gcagcttcgc ggttttatcg aacgcttcac cggcggcatc atcgatagac
     4261 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
     4321 gaaaccagca gcgcgacaaa cggaaattcc ggggggttat cttccagcat cggcgctaac
     4381 agatgccctt ccatatggtg tacagggatc gccggaacgt cccaggcaaa cgccagagaa
     4441 cgccccacgg tcgcgccaac cagtagcgcg ccgactaatc cagggcctgc ggtataggcc
     4501 acagcatcaa tgtcttttgc cgttaaacca gactccttta gcgccgcctg gatcaacggt
     4561 acggttttac gcacatgatc gcgggaggcc agttcaggca cgacgccgcc gtagtcagcg
     4621 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
     4681 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
     4741 tcgctttatt actgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
     4801 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaaggccgcg
     4861 gtgctttaca aagcagcagc aattgcagta aaattccgca ccattttgaa ataagctggc
     4921 gttgatgcca gcggcaaacc gaattaatca aaggtgagag gcacatgccg gtaattaaag
     4981 tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt caagcgttcc tgcgaaaaag
     5041 caggtgttct ggcggaagtt cgtcgtcgtg agttctatga aaaaccgact accgaacgta
     5101 agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa actggctcgc gaaaacgcac
     5161 gccgcactcg tctgtactaa ttccccgaga gcgttgctct ccgatcagac cgagttgtag
     5221 ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt tcgtttatga attgctaaaa
     5281 atcggggcct atggctggac gaatcccacg cgtattcatt aatgatctgc tggcacgcac
     5341 tgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa aagcagggca agaatttcca
     5401 cgcgtgttgt ccattccaca acgagaaaac cccgtccttc accgttaacg gtgagaaaca
     5461 gttttaccac tgctttggat gtggcgcgca cggcaacgcg atcgacttcc tgatgaacta
     5521 cgacaagctt gagttcgtcg aaacggtcga agagctggcg gcaatgcaca atcttgaagt
     5581 gccatttgaa gcaggcagcg gccccagcca gatcgagcgc catcagcggc aaacgcttta
     5641 tcagttgatg gacggtctga atacgtttta ccaacaatct ttacagcaac ctgttgccac
     5701 gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac gaggttatcg cccgctttgc
     5761 gattggtttt gcgccccccg gctgggacaa cgtcctgaag cggtttggcg gcaatccaga
     5821 aaatcgccag tcattgattg atgcgggcat gttggtcact aacgatcagg ggcgcagtta
     5881 cgatcgtttc cgcgagcggg tgatgttccc cattcgcgat aaacgcggtc gggtgattgg
     5941 ttttggcggg cgtgtgctgg gcaacgatac ccccaaatac ctgaactcgc cggaaacaga
     6001 cattttccat aaaggccgcc agctttacgg tctttatgaa gcgcagcagg ataacgctga
     6061 acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg gtggcgctgg cgcaatacgg
     6121 cattaattac gccgttgcgt cgttaggtac gtcaaccacc gccgatcaca tacaactgtt
     6181 gttccgcgcg accaacaatg tcatttgctg ttatgacggc gaccgtgcag gccgtgatgc
     6241 cgcatggcga gcgctggaaa cggcgctgcc ttacatgaca gacggccgtc agctacgctt
     6301 tatgtttttg cctgatggcg aagaccctga cacgctggta cgaaaagaag gtaaagaagc
     6361 gtttgaagcg cggatggagc aggcgatgcc gctttccgca tttctgttta acagcctgat
     6421 gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt ttgagtacgc tggcactgcc
     6481 attgatatcg caagtgccgg gcgaaacgct gcgaatatat cttcgtcagg aattaggcaa
     6541 caaattaggc atacttgatg acagccagct tgaacgatta atgccaaaag cggcagagag
     6601 cggcgtttct cgccctgttc cgcagctaaa acgcacgacc atgcgtatac ttatagggtt
     6661 gctggtgcaa aatccagaat tagcgacgct ggtcccgccg cttgagaatc tggatgaaaa
     6721 taagctccct ggacttggct tattcagaga actggtcaac acttgtctct cccagccagg
     6781 tctgaccacc gggcaacttt tagagcacta tcgtggtaca aataatgctg ccacccttga
     6841 aaaactgtcg atgtgggacg atatagcaga taagaatatt gctgagcaaa ccttcaccga
     6901 ctcactcaac catatgtttg attcgctgct tgaactgcgc caggaagagt taatcgctcg
     6961 tgagcgcacg catggtttaa gcaacgaaga acgcctggag ctctggacat taaaccagga
     7021 gctggcgaaa aagtgattta acggcttaag tgccgaagag cgatcgggaa gcccccgaca
     7081 gccgcactga gaggcagcgg caaatatata agtacgccct cgtaattatc gttggcggta
     7141 aacaaccgtt ggatttcagc gttaacggct gaaggacatc gggtcaatcg cccaacacca
     7201 acctcatgaa ataagtgtgg ataccgtctt atggagcaaa acccgcagtc acagctgaaa
     7261 cttcttgtca cccgtggtaa ggagcaaggc tatctgacct atgccgaggt caatgaccat
     7321 ctgccggaag atatcgtcga ttcagatcag atcgaagaca tcatccaaat gatcaacgac
     7381 atgggcattc aggtgatgga agaagcaccg gatgccgatg atctgatgct ggctgaaaac
     7441 accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc tttccagcgt ggaatctgaa
     7501 atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg aaatgggcac cgttgaactg
     7561 ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg aagacgggat caaccaggtt
     7621 caatgctccg ttgctgaata tccggaagcg atcacctatc tgctggaaca gtacgatcgt
     7681 gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg gctttgttga tccgaacgca
     7741 gaagaagatc tggcacctac cgccactcac gtcggttctg agctttccca ggaagatctg
     7801 gacgatgacg aagatgaaga cgaagaagat ggcgatgacg acagcgccga tgatgacaac
     7861 agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac tgcgcgctca gtatgttgta
     7921 acgcgtgaca ccatcaaagc gaaaggtcgc agtcacgctg ccgctcagga agagatcctg
     7981 aaactgtctg aagtattcaa acagttccgc ctggtgccga agcagtttga ctacctggtc
     8041 aacagtatgc gcgtcatgat ggaccgcgtt cgtacgcaag aacgtctgat catgaagctc
     8101 tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta ccctgtttac cggcaacgaa
     8161 accagcgata cctggttcaa cgcggcaatt gcgatgaaca agccgtggtc ggaaaaactg
     8221 cacgatgtct ctgaagaagt gcatcgcgcc ctgcagaaac tgcagcagat tgaagaagaa
     8281 accggcctga ccatcgagca ggttaaagat atcaaccgtc gtatgtccat cggtgaagcg
     8341 aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact tacgtctggt tatttctatc
     8401 gccaagaaat acaccaaccg tggcttgcag ttccttgacc tgattcagga aggcaacatc
     8461 ggtctgatga aagcggttga taaattcgaa taccgccgtg gttacaagtt ctccacctac
     8521 gcaacctggt ggatccgtca ggcgatcacc cgttctatcg cggatcaggc gcgcaccatc
     8581 cgtattccgg tgcatatgat tgagactatc aacaaactca accgtatttc tcgccagatg
     8641 ctgcaagaga tgggccgtga accgacgccg gaagaactgg ctgaacgtat gctgatgccg
     8701 gaagacaaga tccgcaaagt gctgaagatc gccaaagagc caatctccat ggaaacgccg
     8761 atcggtgatg atgaagattc gcatctgggg gatttcatcg aggataccac cctcgagctg
     8821 ccgctggatt ctgcgaccac cgaaagcctg cgtgcggcaa cacacgacgt gctggctggc
     8881 ctgaccgcgc gtgaagcgaa agttctgcgt atgcgtttcg gtatcgatat gaacaccgat
     8941 cacacgctgg aagaagtggg taaacagttc gacgttaccc gtgaacgtat ccgtcagatc
     9001 gaagcgaagg cgctgcgcaa actgcgtcac ccgagccgtt ctgaagtgct gcgtagcttc
     9061 ctggacgatt aatcggtagg ctggatcagg cgttacgccg cacccggcac ctgtcccttt
     9121 gcacaaacgc caccttttcg gtggcgtttt ttatcgccca cgcaccacca gcgcctggtc
     9181 cagctcgcga tacgcttcga ccagtttctc cagtgaaacg cgacttaaac cgctgggatt
     9241 tggcagcacc caaatctgcg tcgaaccaat gctgagcgtt tgtttccccc actgtgcacc
     9301 gcgctggctg aatccctgtt catatgcttg tttgcccaga atcgccaacg cttgcggctg
     9361 ataatcttca attttttcaa tcagcttgcg cccacctgcg tgtaactctt gctttgaaat
     9421 ttcattggct tgcaccgttg gacggtctac cagtttggtg acgccacaac gataatccag
     9481 cagatgctgt gcctcctgcg gcttcaactg acggtcggta aacccggcct gatatatcac
     9541 cttccagaag cgatttgccg gatgagcaaa gggaaaacca gtcccggcgg atgaaagccc
     9601 agggttgata ccgcaaaaca cgacccgtaa ccctggagcc aaaatatcct caaccatatt
     9661 tactcctttc taacatcacc aggaaaagta taacggattg aaaatacatt gtttataaaa
     9721 acagcaggcg cgcggtaatg gctggattgc gacacggagt tactttataa tccgctacca
     9781 tggcccctta gctcagtggt tagagcaggc gactcataat cgcttggtcg ctggttcaag
     9841 tccagcaggg gccaccagat atagcaaagg ctgacgagaa atcgtcagcc tttttctttt
     9901 acatatcagt tactttgcgt gccagtaggc cgctgcacga acccgctgtg ggtcaaactg
     9961 ttccgtttca aagcggcggc ttaaattctt aacgactttg ccttcgccgg ttatccagat
    10021 gaagtaatca tcggcaggga tttgcatctg cgccagacgc gcatctaccg cctgctcatc
    10081 atgtgccagc cattcgatat taaaaccatc aaggtgcgcg agataatcct gacaggcgtt
    10141 atcccgcacg ctaaccagcg cgctaacttg cggtttaacg gcaagtttgc tcaacgtttc
    10201 caggcggcgg cgcagtgcgg gcattccgga ttcatcgcag acatacagct gatacgcgta
    10261 atcttccggc accaccagcg aaccgcgcgg acctgccacc gtgagtttat cgtccggttg
    10321 ggcctgcatt gcccagctac tggcgacgcc gtcttcgtga ataaagaaat caatcgccag
    10381 ttcatggcgt agttcgtcat acaacggcgt atagtcacgc gacggtgggc gtggtccttc
    10441 cggccagacg atgccctctt ccgttaccgt tggcggcaca aagtgagcgt caggttgagg
    10501 aaagaagagt ttgctgtgat cgtcaaagcc acgcgatgta aaaccgtcca gcgcctcgcc
    10561 gccgaggaca atgcgctgaa aaccggcgct gatgtgctca acgcgtaaca cagtcagttc
    10621 acggaagcgc agatcattgc gaacgcgctg cgggtagcgg ggggagttat tcattgttat
    10681 cgccttcgtg atggtaatca gatatatcta aataattttc ataaatgata atgattgtta
    10741 atcatgataa atgcaagcga tttgtagaac tgatatgtct atagtctgat aagacgaacc
    10801 gcctctcctc aggcatcatt actcaacgcc ggatgcggcg tgaacgcctt atccggccta
    10861 cgtgtgagat gagtccacat ttaaaaatca tttttattct tgcaagagtg ctcatatcag
    10921 atataaatta gatatatcta attaagcaaa aggaggctga tatgagccat catcacgaag
    10981 ggtgttgtaa acatgaaggc cagccacgcc atgagggctg ctgcaaaggt gagaagtcag
    11041 aacacgagca ctgcggacac ggtcaccagc atgaacacgg ccaatgttgc ggtggtcgcc
    11101 acggtcgcgg cggcggtcgt cggcaacgtt tctttggtca cggtgaattg cgtctggtga
    11161 ttctggatat tctctcgcgc gatgacagcc acggttacga gttgattaaa gcgattgaaa
    11221 atctgaccca ggggaattac accccaagcc cgggcgtcat ctacccaacg ctggattttc
    11281 tccaggagca gtcgctgatt accatccgcg aagaagaagg cggcaaaaag cagattgcgc
    11341 tgaccgaaca gggcgcgcag tggctggaag aaaaccgcga acaagtggag atgattgaag
    11401 aacgcatcaa agcgcgttgc gttggcgctg cgctgcgtca gaacccgcaa atgaagcggg
    11461 cgctggataa ttttaaagcg gtgctggatt tacgcgtcaa ccagagcgat atcactgatg
    11521 cacaaataaa aaagatcatt gcggtgatcg accgcgccgc ttttgatatt acgcaactgg
    11581 attaatcacc gattgccgga tgcggcgtac cctacatacc ccgtaggcct gataagatgc
    11641 gccagcatcg catcaggcat tgtgctccaa ccgccgaatc ctgcataccg attaatgcag
    11701 caccgtcacc gcgtcttcca gtcggctggc gcggtgtttc accatcgccg acacctgcgc
    11761 actctcttcc accagctcgg catttttctg ggtgatcagg ttaagctcat ccactgcacg
    11821 ggtcaggctg gaaagcccat cggcctgttc cagcgttgaa tggctaatct gggcaatcaa
    11881 ctgggtgacg tttttcacct gtgccacaat atcttccatc gtccgtccgg cggcgtgtac
    11941 ctgctgcgaa ccggattgca ccttatcagc actggcatca atcagcttgc gaatatcgtt
    12001 ggcagcattg gcgctgcggc tggctaaatg acgcacttcc cccgccacca ctgcaaaacc
    12061 tttgccctgt tcgccggcac gcgccgcttc caccgccgca ttcagggcca gaatattggt
    12121 ctgaaacgca atatcgttaa tcagcgaagt aatggtgcca atgcgctggg tactgtcggc
    12181 gatatcgtcc attgtcttga tcaccgtggt catcgcctcc ccgccctgca ctgccgcatt
    12241 actggcggtg attgacagtt tatcggcagc cgacgctgtg gcagagttct gtttcaccga
    12301 cgccgccatt tggttcatgg tcgccaccgt ttgctgaacg ttatcaactg tctgctgggt
    12361 atgttcgttc agttcatcgg tgcctttcgc cagcgtctca ctgccgtttc tgacactgga
    12421 cacctggctt gagacatcgt taattagcca acggcacatc aggccaagct gccctaccgc
    12481 gcgtaatgtc agccccagct catcgctgcg attcagatgc tcaacactat tacgctctcc
    12541 ggtcgccacc ttcagcgcct gacgggcgac attttctatc gggcggacaa tctgccattc
    12601 aaaacaggcg cttgccaaca ataccactaa cgcacagagg atatacgtca ccaccggggc
    12661 agcaacaaac caaagcatgg ccgccagcaa gataaacatc agggtcatca ctccacgcgc
    12721 ccgccagcga agcggtaatg aaggcagttt acccagccag cctttacgca ccaccaggcc
    12781 tttatgaata cgcttaccgg tacgtccggc gttcagcgct ttgtacagcg gctccaccgc
    12841 cgcgatctct tcatccgtcg cccgggtacg aatcgacata tagccactga tttttccctc
    12901 gcgcaccatc ggtaccgcat tggcccgcac ccaataatgg tcaccatttt tgcggcgatt
    12961 tttcacgatg ccgctccagg gctccccttt tttcagggtg aaccacatat ccgcaaacgc
    13021 cgcttttggc atatccgggt gacgcaccat gttgtgcggc tgcccttgta actcttgcaa
    13081 ggtatagccg ctcacctgca caaaagtgtc attagcatga gtgatatagc tttgcagatc
    13141 ggtagtggac atcagagtgg tatcgtccgc cagcggggta ttttgctggg tgacatacgg
    13201 atgagaagac atgttcgcgt cctgtgcagg ttatatggtt gttaacttct tgtcagagtt
    13261 tatgtcggcc ccgctgcggt tatctttaac cgattaattt gatttagatc gcaatttgcg
    13321 attaaagcac aaatctaatt ccttgattta aaatactttc actctgttac tatacgaaaa
    13381 cgttaattat cttgcccaaa aatcaggcaa ttattgccct gaaaacgtgc atttgcgcag
    13441 caattatcaa atccataccc gacaaaaacc gtgcaaaata acaacgaatg ttaacaaata
    13501 gcattaaata ttgcgcaaat gataaccaca tttgtgttta tcccgatttt cgcgatcgca
    13561 gccggagtgg cgcaatccct gcaatactta aatcggtatc atgtgatacg cgagcctccg
    13621 gagcatattt tgaacaggtt accttcgagc gcatcggctt tagcgtgcag cgcccacgcc
    13681 ctgaatctca ttgaaaagcg aacgctggat catgaggaga tgaaagcact taaccgagag
    13741 gtgattgaat acttcaaaga gcatgtcaat ccggggtttt tagagtatcg caaatctgtt
    13801 accgccggcg gggattacgg agccgtagag tggcaagcgg gaggtttaaa tacgcttgtc
    13861 gacacccagg gacaggagtt tatcgactgc ctgggaggtt ttggaatttt caacgtgggg
    13921 caccgtaatc cagttgtggt ttccgccgta cagaatcaac ttgcgaaaca accactgcac
    13981 agccaggagc tactcgatcc gttacgggct atgctggcga aaacccttgc tgcgctaacg
    14041 ccaggtaaac tgaaatacag cttcttctgt aatagcggca ccgagtccgt tgaagcggcg
    14101 ctgaagctgg cgaaggctta tcagtcgccg cgcggcaaat ttacttttat tgccaccagc
    14161 ggcgcgttcc acggtaaatc acttggcgcg ctgtcggcca cggcgaaatc gaccttccgc
    14221 aaaccgttta tgccactgct gccagggttc cgtcatgtgc cgtttggcaa tatcgaagcc
    14281 atgcgcacgg ctcttaatga gtgcaaaaaa accggtgatg atgtggccgc ggtgatcctc
    14341 gaaccgattc agggcgaagg tggcgtaatt ctgccgccgc cgggctatct caccgccgtg
    14401 cgtaagctgt gcgatgagtt cggcgcactg atgatcctcg atgaagtaca aacgggcatg
    14461 gggcgcacgg gcaagatgtt cgcctgcgaa catgagaacg tacagccgga catcctgtgc
    14521 ctggcaaaag ccctcggcgg cggtgtgatg ccgattggcg caaccatcgc cactgaagag
    14581 gtgttctcag ttctgttcga caacccgttc ctgcatacca ccacctttgg cggcaacccg
    14641 ctggcctgtg cggcggcgct ggcgaccatc aatgtgttgc tggagcagaa cttaccggct
    14701 caggctgagc aaaaaggcga tatgttgctg gacggtttcc gtcaactggc gcgggaatat
    14761 cccgatctgg tacaggaagc gcgtggtaaa gggatgttga tggcgattga gtttgttgat
    14821 aacgaaatcg gctataactt tgccagcgag atgttccgcc agcgcgtact ggtggccgga
    14881 acgctcaata acgccaaaac gatccgcatt gaaccgccac tgacactgac cattgaacag
    14941 tgtgaactcg tgatcaaagc ggcgcgtaag gcgctggcgg ccatgcgagt aagtgtcgaa
    15001 gaagcgtagt atcaaatcgg atggcaatac gacgtcgcca tccgatcttt ttatacgata
    15061 cgtacgcccg caggcatcat ccgctccggc gttaacagca cgctttcact gccgtcatcg
    15121 gtttccgcac acaacagcat gcattccgac gtttcaccac gcattttcgc tttttgcaga
    15181 ttgcataaga ccaccaccgt ttttcccatc aactcttctt cgctgtagta cggcaccagg
    15241 ctggtcacgg tttgcagtgt tttttccccc acatcaacct gtacgatgta cagcttgtcg
    15301 gcgttttcat ggcgtttcac ttccacaatc tttccgacgc gcatttccag acgtgcaaaa
    15361 tcagcgtaag ccacggtttc cattgcttcc tcccctctag taaaatttta ctaaactata
    15421 gcaaaagttt ttctcaatcc tgtaggctaa aaatggagaa tgcaggcgtg atcacattcg
    15481 tgagccgctg tgttaccgtt acagcgtcaa agaaacgcgc tttatttact gaaaacaggt
    15541 gacccgataa gcacttcctc tacaatggaa gcgcacatca gggaaagtaa aaaaggtaaa
    15601 catggcaaca ctaaaagaca tcgcaatcga agctggcgta tccctggcga cagtatccag
    15661 ggtcttaaat gacgatccga cattgaatgt gaaagaagag acgaaacatc gcattctcga
    15721 gatcgccgaa aagctggagt acaagaccag tagcgcccgt aaactacaga caggcgcagt
    15781 caaccaacac catattctgg ctatctacag ctaccagcag gagctggaga tcaacgatcc
    15841 ttactatctg gcgatccgcc acggcattga aactcagtgc gaaaagctgg cgatcgaact
    15901 gaccaactgt tatgaacaca gcggattacc agacattaaa aacgtcaccg gtattttaat
    15961 tgtcggcaaa cccacgcccg ctctgcgcgc cgctgccagc gcgttgaccg acaatatctg
    16021 ttttatcgac tttcacgaac ccggcagcgg ttacgatgcg gtggatatcg atctggcacg
    16081 catcagtaaa gaaatcatcg acttctatat caaccagggc gttaatcgca ttggttttat
    16141 tggcggtgaa gatgagcctg gcaaggcgga tattcgtgag gtcgcctttg cggaatatgg
    16201 ccgtctgaaa caagtagtac gcgaagagga tatctggcgc ggcggttttt ccagttcgtc
    16261 gggttatgaa ctggcaaaac agatgctggc gcgggaagac tatccgaagg cactgtttgt
    16321 tgcttccgat tccattgcta tcggcgtact gcgggcaatt catgaacgtg gcctgaacat
    16381 cccacaggat atttcgctta tcagcgttaa cgatatcccc accgcgcgat ttacctttcc
    16441 gccgctctcc accgtgcgca tccattccga aatgatggga agtcagggcg taaacctggt
    16501 gtatgaaaaa gcccgcgatg gtcgcgcgct gccgctgtta gtcttcgttc ccagcaaatt
    16561 aaaactgcgc ggcacgaccc gttaaatccc cttacacact gtccggcaat cgtttttgcc
    16621 gggcagtgct gccgtttatt ttcgtgatcc agttaaagta aatgcattta cccgctactt
    16681 tttagtaaaa attttactaa actccccagc aattacacaa actaccatcg ccatgaatgg
    16741 ttccgatttc tctctaccgg gaggccctat gaatcgctgg gaaaacattc agctcaccca
    16801 cgaaaaccga cttgcgccgc gtgcgtactt tttttcatat gattctgttg cgcaggcgcg
    16861 tacctttgcc cgcgaaacga gcagcctgtt tctgccctta agcggtcagt ggaatttcca
    16921 cttttttgac catccgctgc aagtgccaga agccttcacc tctgagttaa tggctgactg
    16981 ggggcatatt accgtccccg ccatgtggca aatggaaggt cacggcaaac tgcaatatac
    17041 cgacgaaggt tttccgttcc ccatcgatgt gccgtttgtt cccagcgata acccaaccgg
    17101 tgcctatcaa cgtattttca ccctcagtga cggctggcag ggtaaacaga cgctgattaa
    17161 atttgacggc gtcgaaacct attttgaagt ctacgttaac ggtcagtatg tgggtttcag
    17221 caagggcagt cgcctgaccg cagagtttga catcagcgcg atggttaaaa ccggcgacaa
    17281 cctgttgtgt gtgcgcgtga tgcagtgggc ggactctacc tacgtggaag accaggatat
    17341 gtggtggtcg gcggggatct tccgcgatgt ttatctgatc ggaaaacaac taacacatat
    17401 taacgatttc accgtgcgta ccgactttga cgaagcctat tgcgatgcca cgctttcctg
    17461 cgaagtggtg ctggaaaatc tcgccgcctc ccctgtcgtc acgacgctgg aatataccct
    17521 gtttgatggc gaacgcgtgg tgcacagcag cgccattgat catttggcaa ttgaaaaact
    17581 gaccagcgcc agctttgctt ttactgtcga acagccgcag caatggtcag cagaatcccc
    17641 ttatctttac catctggtca tgacgctgaa agacgccaac ggcaacgttc tggaagtggt
    17701 gccacaacgc gttggcttcc gtgatatcaa agtgcgcgac ggtctgttct ggatcaataa
    17761 ccgttatgtg atgctgcacg gcgtcaaccg tcacgacaac gatcatcgca aaggccgcgc
    17821 cgttggaatg gatcgcgtcg agaaagatct ccagttgatg aagcagcaca acatcaactc
    17881 cgtgcgtacc gctcactacc cgaacgatcc gcgtttttac gaactgtgtg atatctacgg
    17941 cctgtttgtg atggcggaaa ccgacgtcga atcgcacggc tttgctaatg tcgacgatat
    18001 cagccgtatt accgacgatc cgcagtggga aaaagtctac gtcgagcgca ttgttcgcca
    18061 tattcacgcg cagaaaaacc atccgtcgat catcatctgg tcgctgggca atgaatccgg
    18121 ctatggctgt aacatccgcg cgatgtacca cgcagcgaag gcgctggatg acacgcgact
    18181 ggtgcattac gaagaagatc gcgatgctga agtggtcgat attatttcca ccatgtacac
    18241 ccgcgtgccg ctgatgaatg agtttggtga atacccgcat ccgaagccgc gcatcatctg
    18301 tgaatatgct catgcgatgg ggaacggacc gggcgggctg acggagtacc agaacgtctt
    18361 ctataagcac gattgtattc agggacatta tgtttgggag tggtgcgacc acgggatcca
    18421 ggcgcaggat gacaacggca atgtctggta taaattcggc ggcgactacg gcgactatcc
    18481 caacaactat aacttctgtc ttgatggttt gatctattcc gatcagacgc cgggaccagg
    18541 cctgaaagag tacaaacagg ttatcgcgcc ggtaaaaatc cacgcgctgg atctgactcg
    18601 cggcgagctg aaagtcgaaa ataaactgtg gtttaccacg cttgatgact acaccctgca
    18661 cgcagaggtg cgcgccgaag gtgaaacgct cgcgacgcag cagattaaac tgcgcgacgt
    18721 tgcgccgaac agcgaagccc ccttgcagat cacgctgccg cagctggacg cccgcgaagc
    18781 gttcctcaac attacggtga ccaaagattc ccgcacccgc tacagcgaag ccgggcattc
    18841 tatcgccact tatcagttcc cgctgaagga aaacaccgcg cagccagtgc ctttcgcacc
    18901 aaataatgcg cgtccgctga cgctggaaga cgatcgtttg agctgcaccg ttcgcggcta
    18961 caacttcgcg atcaccttct caaaaatgag tggcaaaccg acatcctggc aggtaaatgg
    19021 cgagtcgctg ctgacccgcg agccaaagat caacttcttc aagccgatga tcgacaacca
    19081 caagcaggag tacgaagggc tgtggcaacc gaatcatttg cagatcatgc aggaacatct
    19141 gcgcgacttt gccgtagaac agagcgatgg tgaagtgttt atcatcagcc gcacggttat
    19201 agcaccgccg gtgtttgact tcgggatgcg ctgcacctac atctggcgca tcgctgcaga
    19261 tggccaggtt aacgtggcgc tttccggcga gcgttacggc gactatccgc acatcattcc
    19321 gtgcatcggt ttcaccatgg ggattaacgg cgaatacgat caggtggcgt attacggtcg
    19381 tggaccgggc gaaaactacg ccgacagcca gcaggctaac atcatcgata tctggcgcag
    19441 caccgtcgat gccatgttcg agaactatcc cttcccgcag aacaacggca accgtcagca
    19501 tgtccgctgg acggcactga ctaaccgcca cggcaacggt ctgctggtgg ttccgcagcg
    19561 cccaattaac ttcagcgcct ggcactatac ccaggaaaac atccacgctg cccagcactg
    19621 taacgagctg cagcgcagtg atgacatcac cctgaatctc gaccaccagc tgcttggcct
    19681 cggctccaac tcctggggca gcgaggtgct ggactcctgg cgcgtctggt tccgtgactt
    19741 cagctacggc tttacgttgc tgccggtttc tggcggagaa gctaccgcgc aaagcctggc
    19801 gtcgtatgag ttcggcgcag ggttcttttc cacgaatttg cacagcgaga ataagcaatg
    19861 aggatcatcg ataacttaga acagttccgc cagatttacg cctctggtaa gaagtggcaa
    19921 cgctgcgttg aagcgattga aaatatcgac aacattcagc ctggcgtcgc ccactccatc
    19981 ggtgactcat tgacttaccg cgtggagaca gactccgcga ccgatgcgct atttaccggg
    20041 catcgacgct attttgaagt gcattactac ctgcaagggc agcaaaaaat tgaatatgcg
    20101 ccgaaagaga cattacaggt cgtggaatat tatcgtgatg aaactgaccg tgaatattta
    20161 aaaggctgcg gagaaaccgt tgaggtccac gaagggcaaa tcgttatttg cgatatccat
    20221 gaagcatatc ggtttatctg caataacgcg gtcaaaaaag tggttctcaa agtcaccatc
    20281 gaagatggtt atttccataa caaataacaa ctacggcggc aaaaggaatt tgccgccacc
    20341 gctaccctac tcattttcgg agatgtgtta tgtctgatac caaacgtaat acaatcggca
    20401 aattcggctt gctctcgctg acttttgccg ccgtttacag ctttaacaac gttatcaata
    20461 ataatattga gcttggactg gcctcggcac cgatgttttt cctcgcgacg attttttatt
    20521 ttattccctt ctgtctgatc atcgcagaat ttgtttcgtt aaataaaaac tcagaagccg
    20581 gtgtctacgc gtgggtaaaa agttcgctgg gcggacgttg ggcatttatt actgcctata
    20641 cctactggtt cgtaaacctg ttctttttca cctcgctgtt gccgcgcgtt attgcttatg
    20701 cttcgtatgc cttcctcgga tacgaatata ttatgacgcc ggttgccacc accattatca
    20761 gtatggtgct gttcgccttc tccacctggg tttccaccaa cggggcgaaa atgttggggc
    20821 caattacctc cgtcacttca acgctgatgc tgctgttaac gctctcctac attttactgg
    20881 caggtacggc gctggttggc ggcgtacagc ctgccgatcc gatcaccgtt gacgcgatga
    20941 tcccgaactt caactgggcg ttcctcggcg ttaccacctg gatctttatg gccgcaggtg
    21001 gcgcggagtc cgtcgcggtg tacgttaacg acgtcaaagg cggttcgaaa tcgttcgtta
    21061 aagtgatcat cctcgccggg atttttatcg gcgtactgta ttccgtctcc tcggtgctga
    21121 ttaacgtctt cgtcagcagc aaagagttga aatttactgg cggatcggtg caggtattcc
    21181 acggcatggc ggcgtatttt ggtctgccgg aagcgttgat gaatcgcttt gtcggtctgg
    21241 tgtcctttac cgcaatgttc ggttccctgc tgatgtggac cgcaacgccg gtgaaaattt
    21301 tcttctccga aatcccggaa ggcatctttg gtaagaaaac cgtcgaactg aacgaaaacg
    21361 gcgttccggc gcgcgcagcg tggatccagt tcctgatcgt catcccgctg atgattatcc
    21421 cgatgctcgg ttccaatact gtgcaggatc tgatgaatac tattattaat atgaccgccg
    21481 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
    21541 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
    21601 caatgctgat tgcgatattt tccgtagggt ttgtcgcttc gacattcccg actggcgcga
    21661 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctc ggctttgcgt
    21721 ggtggaaata cagtaaatat ataaagggat taacggctgg agagcgccat attgaagcga
    21781 cgccagccag caatgttgat taagcagact aataaaaaat gagc
//
LOCUS       DAZUCE010000071        21538 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZUCE010000071 DAZUCE010000000
VERSION     DAZUCE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.071"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            231..539
                     /gene="asr"
                     /locus_tag="ACHY03_002221"
     CDS             231..539
                     /gene="asr"
                     /locus_tag="ACHY03_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFS3905444.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            632..715
                     /locus_tag="ACHY03_002222"
     CDS             632..715
                     /locus_tag="ACHY03_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905445.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            815..1636
                     /gene="ydgD"
                     /locus_tag="ACHY03_002223"
     CDS             815..1636
                     /gene="ydgD"
                     /locus_tag="ACHY03_002223"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFS3905446.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQLPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1675..2004)
                     /gene="mdtI"
                     /locus_tag="ACHY03_002224"
     CDS             complement(1675..2004)
                     /gene="mdtI"
                     /locus_tag="ACHY03_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFS3905447.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1991..2356)
                     /gene="mdtJ"
                     /locus_tag="ACHY03_002225"
     CDS             complement(1991..2356)
                     /gene="mdtJ"
                     /locus_tag="ACHY03_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFS3905448.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTHKARKPELEVNHGAV"
     gene            2768..3802
                     /gene="tqsA"
                     /locus_tag="ACHY03_002226"
     CDS             2768..3802
                     /gene="tqsA"
                     /locus_tag="ACHY03_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFS3905449.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3827..5215)
                     /gene="pntB"
                     /locus_tag="ACHY03_002227"
     CDS             complement(3827..5215)
                     /gene="pntB"
                     /locus_tag="ACHY03_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFS3905450.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5226..6758)
                     /gene="pntA"
                     /locus_tag="ACHY03_002228"
     CDS             complement(5226..6758)
                     /gene="pntA"
                     /locus_tag="ACHY03_002228"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFS3905451.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7282..8226
                     /gene="ydgH"
                     /locus_tag="ACHY03_002229"
     CDS             7282..8226
                     /gene="ydgH"
                     /locus_tag="ACHY03_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFS3905452.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8412..9794
                     /gene="ydgI"
                     /locus_tag="ACHY03_002230"
     CDS             8412..9794
                     /gene="ydgI"
                     /locus_tag="ACHY03_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3905453.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTLELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9831..10553
                     /gene="folM"
                     /locus_tag="ACHY03_002231"
     CDS             9831..10553
                     /gene="folM"
                     /locus_tag="ACHY03_002231"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFS3905454.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10550..10885)
                     /gene="ydgC"
                     /locus_tag="ACHY03_002232"
     CDS             complement(10550..10885)
                     /gene="ydgC"
                     /locus_tag="ACHY03_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFS3905455.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11014..11733
                     /gene="rstA"
                     /locus_tag="ACHY03_002233"
     CDS             11014..11733
                     /gene="rstA"
                     /locus_tag="ACHY03_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFS3905456.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALQFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11737..13038
                     /gene="rstB"
                     /locus_tag="ACHY03_002234"
     CDS             11737..13038
                     /gene="rstB"
                     /locus_tag="ACHY03_002234"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFS3905457.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPNKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13114..14043
                     /gene="tus"
                     /locus_tag="ACHY03_002235"
     CDS             13114..14043
                     /gene="tus"
                     /locus_tag="ACHY03_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFS3905458.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVTQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(14040..15443)
                     /gene="fumC"
                     /locus_tag="ACHY03_002236"
     CDS             complement(14040..15443)
                     /gene="fumC"
                     /locus_tag="ACHY03_002236"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFS3905459.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15586..17232)
                     /gene="fumB"
                     /locus_tag="ACHY03_002237"
     CDS             complement(15586..17232)
                     /gene="fumB"
                     /locus_tag="ACHY03_002237"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFS3905460.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17431..18606
                     /gene="manA"
                     /locus_tag="ACHY03_002238"
     CDS             17431..18606
                     /gene="manA"
                     /locus_tag="ACHY03_002238"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFS3905461.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HKSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18707..20215
                     /gene="ydgA"
                     /locus_tag="ACHY03_002239"
     CDS             18707..20215
                     /gene="ydgA"
                     /locus_tag="ACHY03_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFS3905462.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEEMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(20260..21525)
                     /gene="uidC"
                     /locus_tag="ACHY03_002240"
     CDS             complement(20260..21525)
                     /gene="uidC"
                     /locus_tag="ACHY03_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFS3905463.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWGQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNLFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         5384 a         5559 c         5188 g         5407 t
ORIGIN      
        1 ccgctttgta agtagtaaaa tagttaaccc gatcaagact actattattg gtagctaaat
       61 ttcccttaag tcacaatacg ttattatcaa tgctgtaatt tattcagcgt ttgtacatat
      121 cgttacacgc tgaaaccaac cactcacgga agtctgccat tcccagggat atagttattt
      181 caacggcccc gcagtggggt taaatgaaaa aacaaattga gggtatgaca atgaaaaaag
      241 tattagctct ggttgttgcc gctgctatgg gtctgtcttc tgccgccttt gctgcagaga
      301 ctgcgaccac acctgctccg actgcgacga ccaccaaagc agcgccggcg aaaactacac
      361 atcataaaaa acagcataaa gcagcacctg cccagaaagc gcaggcggct aaaaagcatc
      421 ataaaaatac gaaagctgaa cagaaagccc ctgaacaaaa agcgcaggca gcgaagaaac
      481 acgccgggaa acacagccat cagcaaccgg caaaacctgc tgcacaaccc gcagcgtaag
      541 ttttcaacag taatgctggc gcgccccctc gcgcctgaaa attacggtgc taagcgggta
      601 acgtttagca ccgcctttag cggagggtag tatggtgggc cgttatcgct ttgagttcat
      661 tcttatcatc cttattttat gcgcactgat tgccgcccgt ttttatcttt cctgactgta
      721 gttatctgat tttactccca ctttcatccc gtcccgtcta tagtatttat gagggtttgc
      781 ttttaataat cataattacc caccagagtg tgatatgcgt acaaccattg ctgtagtgtt
      841 gggtgcaatt agtttgacgt ctgcttttgt gtttgcagat aaaccagacg ttgccaaatc
      901 ggcaaacgat gaggtcagca ccctgttttt tggtcatgat gatcgtgtgc cagtgaatga
      961 cacgacccaa ttaccgtggg atgcggttgg gcaactggaa acggccagcg gcaatttatg
     1021 tacggcgacg ctgattgcac ccaatctggc attaacggca ggacactgtt tattgacacc
     1081 tccaaagggt aaagccgata aagcagtggc gctgcgtttt gtgtcaaata aaggtctttg
     1141 gcgctatgac atacacgaca tagaaggccg cgttgatccg acactgggaa agcggttaaa
     1201 agcagatggg gatggttgga ttgtacctcc cgcagccgcg ccgtgggact tcggattgat
     1261 tgtgctacgt aatccccctt ctggcattac gccgttgccg ttatttgagg gagataaagc
     1321 cgcgcttacc gccgcattaa aagcggcagg tcgtaaagtg actcaggcag gataccctga
     1381 agatcatctc gatacgttgt acagtcatca aaactgtgaa gtgactggct gggcgcaaac
     1441 gtcggtgatg tcacatcagt gcgatacctt gccgggtgac agcggttcgc ctctgatgtt
     1501 gcataccgat gacggctggc aattaattgg ggtgcaaagt tcggctcctg ccgcgaaaga
     1561 tcgctggcgc gccgataacc gggccatttc tgttaccggt tttcgcgaca agctggatca
     1621 actgtcgcaa aaataatgtt caaacgctgc ccgacagcgc gggcagcgtc ttcatcaggc
     1681 aagtttcacc atgatcattc cagccaacag caagaccagg ccaatccagc ctttacgatt
     1741 taaccgttga ccaaacaaga tccaacctgc ggctaacgtg gcggcaatac caaacccgcc
     1801 ccacaatgca taagcgacag acaagtcgat ccctttaacg gcctgagaaa gcgcactaaa
     1861 ggcagccagc accgccgcca gggagagcaa gccaaatatt ttgcgacgaa agccgtcaga
     1921 aaatttcaaa aagacgttag caacgatttc cagcacgatt gccaatgcca gccaggcggc
     1981 gtgaacccat tcaaactgcg ccatggttca cctccagttc aggtttacgc gctttatgag
     2041 tacctgattt gatcaacact atcccggcga ccagggtggt taacccggca attttcatca
     2101 gcgataaact ttcgtcgaat aacaaaacgc taaacaaggt aataaataaa ataccgatac
     2161 cttcccacag cgcataagcc acgcctaagg ctattttttt aacagcgaaa gagagaaata
     2221 tatacgacag agaaatcatc accagcatta aaataaagcc gccatttccc tcactgacgc
     2281 tcgcccattt catagacagc gtaccggtaa tttctgtagc aatagccaga cctaataaaa
     2341 tccaataaat atacattgtc cttctcctgc aagagaatta ttttaatttt cgcttaattc
     2401 agcgaaacca aagtaaagtg gtcaagcccc gcgcttagcg caagctttgg cagaagaaag
     2461 gactaaagcg cgttgcgcca gtgctgctca cctacgagca agatagtgga tgaggtacga
     2521 agatgggtaa atgtagaaaa taacgtcctg aacaaattgt ccataatatt acaattatcc
     2581 gcagtgttgc ttctcgtcat cgcggatgat aattgtcctc ggtagttgaa cacgcctgat
     2641 ttgtatcata gcttaagaat taactcaaaa tattttcatt tctttacctg agcggtttga
     2701 ttttcgttat gatgatggag cgaaaaagac attattattt gcaaaggaag aaaaaacggg
     2761 gacaagcatg gcaaagccga tcatcacgct caatggccta aaaatcgtca ttatgttggg
     2821 aatgctggtc attattctct gcggtatccg ttttgccgcc gagatcatcg tgccgtttat
     2881 tctcgcatta tttattgctg ttattcttaa cccgctggtg caacacatgg tccgctggcg
     2941 agtgccgcgt gtactggcgg tgtcgatttt gatgaccatc atcgtgatgg ctatggtgtt
     3001 gctgttagct tatctgggtt ccgcgctcaa cgagttgacg cggacgttac cgcaatatcg
     3061 caactctatt atgacgccgc tgcaagctct tgagccgttg ttgcaacgcg tagggattga
     3121 cgtctcagtt gaccaactgg cgcattacat tgatccgaac gcggcgatga cgttgctcac
     3181 caacttattg acgcagttat ctaatgccat gtcatcaata tttttattgc tgctgacggt
     3241 gctgtttatg ctgctcgaag tgccacaatt gcccggaaaa tttcagcaaa tgatggcgcg
     3301 tccggttgaa gggatggcgg cgattcagcg tgcgattgac agtgtgtctc attatctggt
     3361 gctgaaaaca gccatcagca tcatcaccgg cctggtcgca tgggcgatgc tcgccgccct
     3421 cgatgttcgc ttcgcttttg tctggggatt gctggccttt gcgcttaatt acatccctaa
     3481 tattggttca gttctcgcgg caatcccccc tatcgctcag gtactggtgt ttaatggctt
     3541 ctacgaagcg ttgctggtgc tggcgggata tctgctaatt aatctggtct tcggcaatat
     3601 tctggagccg cgcatcatgg gacgtgggct ggggctttcc acattggtgg tatttttgtc
     3661 gttgattttt tggggatggt tgttaggacc ggtgggtatg ctgctttccg tgccgttgac
     3721 aattattgtc aaaattgcgc ttgaacaaac agcgggaggt caaagcatcg ccgttctgtt
     3781 aagcgatctc aataaagagt gacggcctcc gcagaggccg tcagggttac agagctttca
     3841 ggattgcatc cacgctggct ttggcgtcac caaacagcat gtgggtgttt tccttgaaga
     3901 acagcgggtt ttgcacacca gcatagccag tgttcatcga acgtttaaag acaatcacgt
     3961 tctgcgcttt ccacacttcc agcacaggca taccagcaat cggactcttc ggatcatcct
     4021 gcgccgccgg gttaaccgta tcgttagcac caatcaccag tacggtatcg gtatcagcaa
     4081 agtcatcgtt gatctcgtcc atttccagca cgatgtcata cggtactttt gcttcagcca
     4141 gcaatacgtt catatgtcca ggcaaacgcc ccgcaaccgg gtggataccg aaacgcacgt
     4201 tgataccacg agcgcgcagt ttctcggtaa tttcagcgac aggatattgc gcctgcgcga
     4261 ctgccatgcc gtaccccgga gtaatgatca ctgaatggga gtttttcaac agttccgctg
     4321 tctcttctgc ggtgatttcg cggtgctcac ccacttcctg atcatcgcca gtagaagagc
     4381 cgtcggtgcc gaaaccaccc gcaataacgc tgataaagga acggttcatc gccttacaca
     4441 taatgtaaga aaggatagcc cccgaggaac cgaccagcgc accggtcaca atcagcaggt
     4501 cgttgctgag cataaagccc gcagccgcag ccgcccagcc ggagtacgag ttcagcatcg
     4561 acaccaccac cggcatatct gcaccaccga tggaggcgac taaatgccag ccaaatacca
     4621 gcgcaatggc ggtcattatc agcaatgcca gcacttgcag gccgacgctg tccgtgcgca
     4681 caaatacaat cagcagcagg aaggaaacga ccagagccgc caggttcatt ttatgacggt
     4741 ttggcagcat caacggttta gacgaaatct tgccacacag tttgccgaac gccaccaccg
     4801 aacccgtgaa cgttaccgcc ccgatgaaga taccgaggaa cacttccgtc aggtgaatat
     4861 tgaccagaat cggtgccatt cccgcgtcat gatgcagata gctgttaaag ccaaccagca
     4921 ctgccgccag acctacgaag ctatgcagga tcgccaccag ttctggcatt tcggtcattt
     4981 caactttctt cgccagacgg ataccaattg ccccaccaat gaccatcgcc agcaagatcc
     5041 agccaacgtt acccgtatcc ggtccaaaaa tggtggcgat tagcgcaatc gccatcccgg
     5101 cgataccgaa gttgttaccc tggcgagacg tttcatgttt cgaaagaccg gccagactga
     5161 agataaacag gatcgcggca acaatgtatg cagctgtaac taatcctcca gacatatgtt
     5221 accccttaat ttttgcggaa cattttcagc atgcgctgag tcacggtgaa gccaccgaaa
     5281 atattaatgc tggctataag caccgcgata aaactaagga agctaaccca gccgccctgg
     5341 ccaatctgca acagtgctcc gacaacaata atccctgaaa tcgcgttggt gaccgacatc
     5401 aacggtgtat gcagcgcgtg cgatacattc cacaccacgt aataaccgac aacgcaggcc
     5461 agcgcgaaaa cggtgaagtg cccaagaaac tctttcggcg caacgcttgc cagccagccg
     5521 aaaaggatga ttgccagcgc catcaacgcg tatttacgcc acggtgaaca ggcacatttt
     5581 tcctcagttt tcacttccgg tgccgctttt tgtgccgcct gcggctgagc tgatacctga
     5641 atcggcggtg ccggccaggt aatttcgccc gcacggatca cggttacgcc gcgaatcacc
     5701 acatcatcaa aatcaacagt gatgttgccg tctttctctt tacacaacag tttcagcaga
     5761 ttaacgaggt tagtgccgta aagctgtgag gattgcgtcg gcagacggcc cggaagatcg
     5821 gtataaccaa ttactttgac accattttcc gtagtgaaga tttcacccgg cacggtgtat
     5881 tcacagttgc caccgttttg ggctgccagg tcgacaatca cactgcccgc cttcatggag
     5941 tcaaccattt cacgggtaat tagcttcggc gctggtttgc ctggaataag cgcggtggtg
     6001 acaataatat cgacctcttt tgcctgggcg gcaaagagtt ccatttccgc tttgatgaac
     6061 gcgtctgaca tcactttggc atagccatcg ccgctgcccg cttcctcttt aaaatccagc
     6121 tcgaggaatt ccgcgcccat actttgaact tgttctttca cttccgggcg ggtgtcgaat
     6181 gcacgcacaa tcgcgccgag actgtttgct gcgccaatgg cggccagacc tgcaacaccc
     6241 gcaccaatca ccatcacttt tgccggtggc actttcccgg ccgcagtgat ttgcccggta
     6301 aagaagcgcc caaattcatg tgccgcttca acaatggcgc gataaccggc gatgttcgcc
     6361 atcgagctta gtgcgtccag cgattgtgcg cgtgagatac gcggcacaga gtccatcgcc
     6421 atcacggtca cgttacgttc cgcaagtttt tgcattaatt ccggattctg cgcaggccag
     6481 ataaaactca ccagcgttgt cccaggattc agtaacgcaa tttcatcatc taacggcgca
     6541 ttgaccttca gaatgatctc tgactgccag acgctattcc cttctacaat ttctgcgccc
     6601 gcttgcacaa acgctttatc gtcaaaactt gccagttgac ccgcgccgct ctctaccgcg
     6661 acggtaaaac ccagtttcag cagctgttcc actgtttttg gcgttgctgc aacacgggtt
     6721 tcattggtta accgttctct tggtatgcca attcgcatga tattcccttc catcggtttt
     6781 attgatgatg gtttgcctgt gtcaggagcc acacaagccg ctcatgtacg agctaaatgt
     6841 tactccgtta aaataaatta gtaacaaacg ccctataacg tactgaaaat tatgcctgtg
     6901 atctagcgcc aaaaatcagt atttcggcgt gaactcgcaa aatattaacg attcagccgt
     6961 gatagtggga taaacacctt agaacgccgg ataaagactg ataattgtct tcgacggtcg
     7021 ggtaaaacga gacatcgccc cggcacgaat cactacttaa cattaaatta acttatacaa
     7081 ttcagttgct tcagtagtaa tgatgctgat acggctgttt tttaagcata gacggtcatt
     7141 tgagcaggat taaaattggc ttaaggaatg tgatatgaaa aatgacgcag acagttacac
     7201 cgtttaaatg caataatcag ccacgtttct cgttaataac aataccagta cctggtttgc
     7261 gcaaggcgaa ggattatttt tatgaagctt aagaacaccc tcctggcgtc ggcactgctt
     7321 tctgctacgg cattctccgt taacgcagca acagaactga caccggagca agcggcagcg
     7381 gttaaacctt ttgaccgtgt agtggttacc ggtcgtttta atgctattgg cgaagcggtg
     7441 aaagccgttt ctcgtcgcgc agataaagaa ggtgccgcct ctttttatgt tgtcgacact
     7501 tctgattttg gtaacagcgg taactggcgt gtggtcgctg acctctataa agccgatgct
     7561 gaaaaagcag aagaaacaag taatcgcgta attaacggtg ttgtcgaact gccgaaagat
     7621 caggctgttc tgattgaacc gtttgacacg gtcaccgtcc agggcttcta tcgtagccag
     7681 ccagaagtca atgatgccat caccaaagcg gcaaaagcga aaggtgccta ctctttctac
     7741 atcgttcgtc aaatcgatgc caaccagggc ggcaaccagc gtattactgc attcatctat
     7801 aaaaaagatg ctaagaaacg tatcgtccag agcccggatg tgatcccggc agattccgaa
     7861 gcaggacgtg cagctctggc tgccggtggc gaagccgcga agaaagttga gatcccgggt
     7921 gttgcgacta ccgcatcacc aagttctgaa gtcggtcgct tctttgaaac ccagtcatca
     7981 aaaggcgggc gttacaccgt cacgctcccg gatggcacta aagtcgaaga actgaacaaa
     8041 gcgacagcag cgatgatggt cccgttcgac agcatcaaat tctctggcaa ctacggcaac
     8101 atgaccgaag tctcctatca ggttgcgaaa cgtgccgcga agaaaggtgc taagtattac
     8161 cacatcaccc gccagtggca ggaacgtggt aataacctga ccgtcagtgc agatctgtat
     8221 aaataactat tcagttactc ttataaggcg gcttgatgcc gcctttcgca tttttagtca
     8281 caaatttccc cgcgaccatt gcatgcagac acatctcttc gtaaaatccc gcgcttcagt
     8341 ggcaaactcc atttttatgc gttttgccct aatatttatt cttcttatca cgttttaatc
     8401 actggatatc gatggaaaag aaactgggac tgagcgcact caccgcgctg gtattaagct
     8461 caatgctggg cgcgggtgtt ttcagtctgc cgcaaaatat ggcggcagtt gccagcccgg
     8521 cagcactgct catcggctgg ggtattactg gcgctggcat tttattgctg gcctttgcca
     8581 tgctgatcct cacgcgcatt cgtcccgaac ttgacggcgg tatctttacc tatgcccgcg
     8641 aagggtttgg cgagctaatc ggcttttgtt ccgcatgggg atactggctg tgtgcagtca
     8701 tcgccaacgt ctcctatctg gtgatcgttt tttccgcgtt aagctttttt acggacacgc
     8761 tggaattgcg cctgtttggc gatggtaata cctggcaatc gatagtcggt gcatcggctt
     8821 tattgtggat tgttcacttt ttgatcctgc gcggagtgca aaccgctgcc agcattaacc
     8881 tggtggcgac actggcaaaa ttgttgccgc tgggtctgtt tgttgtgctg gcgatgatga
     8941 tgttcaaact ggataccttc aagctcgact tcaccggact tgcacttggc gtacccgtgt
     9001 gggaacaggt gaaaaacacc atgctgatca ccctgtgggt tttcattggt gtggaaggtg
     9061 ccgtggttgt ttctgcgcgt gcgcgtaata aacgtgatgt tggcaaagcg acactgctgg
     9121 cggttctctc cgctctgggc gtttatttgc tggtaacgct gctttcgctg ggcgtggtgg
     9181 cacgtcctga actggctgaa attcgtaacc cgtcaatggc cggactgatg gtggaaatga
     9241 tgggaccgtg gggcgaaatc atcatcgctg ccggtttgat cgtttccgtt tgcggcgcgt
     9301 acttaagctg gaccatcatg gcggcagaag ttccgttcct ggccgccact cataaagcat
     9361 tcccacgcat ttttgcgcgc cagaacgccc aagctgcgcc atccgcttca ctgtggctga
     9421 ccaacatctg cgtgcaaatt tgtctggtac tcatctggct gaccggttcc gattacaaca
     9481 cgttgctgac catcgcctca gaaatgattc tggtgcccta tttcctggtc ggcgcgttct
     9541 tgctgaaaat cgcaacccgt ccactgcata aagcggtagg tgtcggtgcc tgcatttatg
     9601 gcttatggtt attgtacgcc tctggtccaa tgcacttgct gttatccgtt gttctgtatg
     9661 caccggggct gctggttttc ctgtatgcgc gcaaaacgca tacacatgat aatgtactga
     9721 accgtcagga aatggtgctg attggcatgc tgttaattgc ctcagttccg gcgacctgga
     9781 tgctggtggg ataacgttca tcccatcgtt ggatagcaaa ggagataacg atgggtaaaa
     9841 cccagccctt gccaatatta attactggcg gaggtcgtcg catcggcctc gccctcgcat
     9901 ggcatttcat taatcaaaag caaccggtga ttgtcagcta tcggacacac tatccagcca
     9961 ttgatggact gattaatgca ggtgcgcagt gtattcaggc tgatttttcg accaacgacg
    10021 gtgtgatggc gtttgccgat gaagtactaa aaagcaccca tggtctgcgt gctattttgc
    10081 ataacgccag tgcgtggatg gcggaaaaac cgggtgcgcc actggccgac gtactggctt
    10141 gcatgatgca gatccacgtt aataccccat acctgctcaa ccatgcgctg gaaagattac
    10201 tgcgtgggca cggacacgcc gccagcgata tcattcactt taccgattat gtggtggagc
    10261 gcggtagcga caaacatatt gcgtatgctg caagcaaagc ggcactggat aatatgaccc
    10321 gctcgtttgc ccgcaagctg gcaccggaag tgaaagtgaa ttctattgcg ccatcgctga
    10381 tcctgtttaa tgaacatgat gatgccgaat atcgacaaca ggcgctgaat aaatcactga
    10441 tgaaaaccgc gcctggcgag aaagaagtga tcgacctggt cgattactta cttaccagtt
    10501 gctttgtcac cggacgcagt ttcccacttg atggcggtcg tcatctgcgt taatgcagct
    10561 ttatccagca tataatcaac acccatgcgc ttatccccca acacgcaaca gaaccaacaa
    10621 aagcggcggg caagcgcatc attccggtga aataccacag cgacaccagg taaacaaaat
    10681 agggaataat cgaccacata ctaaaaatga tggttgcgcg taaggcttca atgccgcgtt
    10741 cgctggcaac aatataatgc gcgatgagcg caaaggtcgg gaaaagtggg atcagcccgg
    10801 cgatataata atttttcgtt tttgctaaaa caccaatcaa cagcactacc agcgcaccga
    10861 gcgcggcttt gattaccagc cccatctttt taccttaaca cttccataac aagtcatcag
    10921 tagaatacct gatgaaaact tgtttagaaa cgattgatag taagtaaaaa cagcgcggtg
    10981 tattgtgacg tttttatatc taccgtgaat gttatgaaca ctatcgtatt tgtggaagat
    11041 gatgcggaag tcggttcact gattgccgcg tacctggcaa aacatgatat gcaggttacc
    11101 gtagagccgc gcggcgacca ggccgaagaa accattttgc gagaaaatcc ggatttggtg
    11161 ttactcgaca tcatgctacc aggcaaggac ggcatgacca tttgtcgtga tttacgcgca
    11221 aagtggtctg gaccgattgt tcttctaacc tctctcgata gcgatatgaa ccacatcctg
    11281 gcactggaaa tgggtgcctg cgactatatt ctcaaaacga cgccccctgc tgttttgcta
    11341 gcgcgtttac gtttgcattt gcgtcagaat gagcaagcca cactgaccaa aggtcttcag
    11401 gaaacgtctc tgactcccta caaagccctg caattcggca cgttgaccat cgatcctatc
    11461 aaccgcgtag tcaccctggc taacactgaa atctcgctct cgacagctga tttcgaatta
    11521 ttgtgggaat tagctaccca tgccgggcaa atcatggacc gcgatgcatt gctgaaaaat
    11581 ttacgcggcg tcagttatga cggactggat cgtagcgtgg acgtggctat ttcgcggtta
    11641 agaaaaaaac tgctcgataa cgccgcagaa ccttatcgca ttaaaactgt gcgtaacaaa
    11701 ggctatcttt ttgcgcctca tgcatgggaa taagcgatga aaaaactgtt tatccagttt
    11761 tacctgttat tgtttgtctg cttccttgtg atgtctctgc tggttgggct ggtgtacaaa
    11821 tttaccgccg aacgcgcggg caaacagtcg ctggatgatt tgatgaacag ttcgctgtat
    11881 ctgatgcgca gcgaattacg tgagatcccc ccacacgact ggggtaagac actgaaagag
    11941 atggatttaa atctctcttt cgatctgcgt gtcgagccac tgagtaaata ccatcttgat
    12001 gatatttcca tgcaccgact gcgtggcggc gaaattgtcg ccctggacga tcagtacacg
    12061 tttttgcagc gtatcccgcg cagccactac gtgctggcag ttggtcctgt tccttatctt
    12121 tattacctcc atcagatgcg attgctggat atcgccctga tcgcttttat tgctatttcc
    12181 ctcgcctttc cggtgtttat ctggatgcgt ccgcactggc aggatatgtt aaaactggaa
    12241 gcagcggcgc aacgatttgg cgatgggcat ctcaatgaac gtatccactt tgatgagggt
    12301 tcgagctttg aacgacttgg tatcgcattt aaccagatgg cggacaatat caacgccttg
    12361 attgccagca aaaaacagct tattgacggt atcgctcacg aactgcgaac accgttagtg
    12421 cgcctgcgtt atcgactgga gatgagcgat aacctgagcg ccgccgaatc ccaggcgttg
    12481 aatcgtgata tcagtcaact tgaagcttta attgaagagc tgctgactta tgcccgactc
    12541 gatcgcccac aaaacgagct tcatcttagc gaaccagacc tgccgttgtg gctatcaacg
    12601 catctggcag atattcaggc agtaacgccc aataaaacgg tacggataaa aacgctcatg
    12661 caaggccatt atgcggcgtt ggatatgcgc ttaatggagc gcgtgctgga taatttgctc
    12721 aataacgccc tgcgctactg ccattcaacg gttgaaacca gcctgctact gtcggggaat
    12781 agagcgacat taattgttga ggatgatggc ccagggattg ccccagaaaa ccgcgaacat
    12841 atctttgaac cttttgttcg cctcgatccc agccgggatc gctcaaccgg cggctgcggg
    12901 ctggggctgg caattgtcca ctctatagca ctggcaatgg gcggtacggt taattgtgac
    12961 accagcgaac tgggtggtgc ccgcttctcg tttagctggc cgttatggca taacatcccg
    13021 caatttacct ctgcttgaca ctacgcgcac ggtggtcaag tcaccaccac tgtgctataa
    13081 attaaggatg ttgtaactaa agtggttaat attatggcgc gttacgatct cgtagaccga
    13141 ctcaacacta cttttcgcca gatggaacaa gagctggctg catttgccgc tcatcttgag
    13201 caacacaagc tattggttgc ccgcgtgttc tctttgccgg aggtaaaaaa agaggatgag
    13261 cataatccgc ttaatcgtat tgaggtgaca caacatctcg gcaacgacgc gcagtcgctg
    13321 gcgttgcgtc atttccgtca tttatttatt caacaacagt ccgaaaatcg cagcagcaaa
    13381 gccgctgtcc gtctgcctgg cgtgttgtgt taccaggtcg ataacctttc gcaagcagcg
    13441 ttggtcagtc atattcagca catcaataaa ctcaagacca cgttcgagca tatcgtcacg
    13501 gttgaatcag aactccctac cgcggcacgt tttgaatggg tgcatcgtca tttgccgggg
    13561 ctgatcaccc ttaatgctta ccgcacgctc accgttctgc acgaccccgc cactttacgt
    13621 tttggctggg ctaataaaca tatcattaaa aatttgcatc gtgatgaagt cctggcacag
    13681 ctggaaaaaa gcctgaaatc accacgcagt gtcgcaccgt ggacgcgcga ggagtggcaa
    13741 agaaaactgg agcgagagta tcaggatatc gctgccctgc cacagaacgc gaagttaaaa
    13801 atcaaacgtc cggtgaaggt gcagccgatt gcccgcgtct ggtacaaagg agatcaaaaa
    13861 caagtgcaac atgcctgccc tacaccgctg attgcactga ttaatcggga taatggtgcg
    13921 ggcgtgccgg acgttggtga gttgttaaat tacgatgccg acaatgtgca gcaccgttat
    13981 aaacctcagg cgcagccgct tcgtttgatc attccacggc tgcacctgta tgttgcagat
    14041 taacgcccgg ctttcatact gccgaccatc tgttctggcc gtacccagct gtcaaactcg
    14101 gcttcgctaa gataccccag cgcaagggcc gcagctttta aggtcagccc ttctttatgc
    14161 gcttttttgg cgatctccgc ggctttgtca taaccaatgt gggtgttaag cgcagtcacc
    14221 agcatcagcg attcattgag taattgattg attcgctcac gattcggttc aatacccact
    14281 gcgcagtgtt tgttaaaact ttccatgcca tctgccagca agcgcaccga ttgcaggaaa
    14341 ttgtggatca ccattggacg gaagacgttc agttcaaagt taccggaagc gccccccatg
    14401 ttgatcgcca cgtcgttccc catcacctga cagcagagca tggttaatgc ctcacactgt
    14461 gttgggttca ctttccccgg catgattgag ctgcccggct cattttccgg gattgagatt
    14521 tcaccaattc cgcagcgcgg gccagaggcc agccagcgga catcattggc gattttcatc
    14581 agtgacgcag ccaacccttt caacgcgccg tgcgcctgaa ccagggcatc acaggtcgcc
    14641 agcgcttcaa atttgttcgg cgcggtaaca aacggtgcac aggtaatgac tgccagttca
    14701 tctgctacgc gacgcgcata ctccggatgg gtatttagtc cagtacccac cgctgtaccg
    14761 ccaagagcca gttccgctac gtgaggcagg ctgtattcga tatgtttgag attatgctcg
    14821 agcatcgcta cccagccgga aatctcctgc cccagtgtta acggcgtggc atcctgcaag
    14881 tgagtacgac caattttgac gatatcggca aatgcacggg atttctcatt cagtgtctgt
    14941 gtcagggttt taagctgagg aatgagttgc ttgcgcagcg ccagcagcgc cgcaacgtgc
    15001 atcgccgtcg gaaagacatc gttggaactt tggcttttgt tcacgtcgtc gttagggtga
    15061 actttacgtt ccatcccgcg cacgccgccg agtaattcac tggcccggtt agccagcact
    15121 tcgttcatgt tcatgttact ttgcgtgccg gagccggtct gccagatagc cagcgggaat
    15181 tcgtcgtcat gctgtcctgc cagtacttca tccgccgcct gccgaatggc gctcgctttc
    15241 tcttcagaca acaagcctaa atcttcatta acttttgccg ctgcgcgctt ggttagcgcc
    15301 agcgcatgaa tcagtgaggt gggcattttc tccgtcgaaa tgcggaaatg ctccagcgag
    15361 cgttgagttt gtgcgcccca cagcttatct gccgggacat caatcgcccc catcgaatct
    15421 ttttcgctgc gtactgtatt catggcctgc tcctcacctg attaattttt tctttctgtt
    15481 ttgctttcgt taagcaactt ttagctcact cattatttac tatttgataa caaatgtttg
    15541 gtctttcgtg ccatgtaaaa aaaccgcccc gaagggcggc tctgtttatt tcacacagcg
    15601 ggtgcattgt gtgagttgta tctgctggaa gaagtcattt cctttatcat ccacaaggat
    15661 aaacgccggg aaatcttcca cttcaatttt ccagatggct tccattccca gttccggata
    15721 ttcaacacat tccaggctct taatacttcc ctgcgccaat acagcggccg gaccaccgat
    15781 actgccaagg tagaagccac cgtgtttttt acaggcatcc gtcacctgct ggctgcggtt
    15841 gcctttcgcc agcatgatca tacttccgcc ctgcgcttgc agttgatcga cataagaatc
    15901 catccgtccg gcggtcgttg ggccaagaga accggaggca taaccttccg gcgttttggc
    15961 cggacccgcg tagtaaatcg gatgatcttt gatgtactgc ggcagccctt caccgttatc
    16021 catccgctct ttcagtttgg cgtgagcaat atcacgaccg acgataatcg tgccgttaag
    16081 tgataagcgt gtagaaacgg gatactgcga caactgtgcg aggatctctt tcatcggacg
    16141 gttaaggtca acgcgcaccg cttcgccttc tcccgctttg cgcagctctt ccgggatata
    16201 tttgcctgga ttatgttcca gtttttcgat ccagatcccc tgacggttga tcttcgcttt
    16261 gatattacgg tcagcagagc aggagacgcc cataccgacc gggcaggatg cgccgtgacg
    16321 tggcaggcga atcacgcgga tgtcgtgagc gaagtattta ccaccaaact gcgcacccag
    16381 accaagattt tgcgcttcga tcagcaattc tttttccagt tccacatcgc ggaacgcctg
    16441 accgtgctca ttcccttccg ttggcagttc atcatagtat ttcgcggaag ccagtttcac
    16501 cgttttaagg ttcgtttctg cagaagttcc accaataacg aacgcaatat gatacggagg
    16561 acaggccgcc gtacccagcg tgcgcatctt ctcaaccagg taatttttca gtttccccgg
    16621 cgtcagtaac gctttggttt cctgatagag atacgtcttg tttgccgaac caccaccttt
    16681 ggcgatacag aggaatttgt actcgtcgcc atcaacggca taaagatcga tctgcgctgg
    16741 tagattggtg ccggtattca cctctttata catatccagc ggcgcgtttt gcgagtagcg
    16801 cagattatct tcgatataag tgttatagac accgcgtgcc agcgccgctt catcaccacc
    16861 accggtccat acacgctgcc cttttttacc aacaataatc gcggtgccgg tatcctgaca
    16921 ggttggcaga acgcctttcg ccgcgatgtc ggagttacgc aggaattgca gcgccacata
    16981 tttatcattt tcgctggcct ccgggtcacg cagaatgtcg gccacctgtt gttggtgcgc
    17041 cggacgcagc atgaacgacg catcatgaaa cgcctggcgc gccaacagag ttaacgcttc
    17101 gggtgcgact ttcaaaatct cctgcccttc aaattcagat acgctaacgt gttcgctggt
    17161 tagcaggtaa tactcagtat catctttttt gagtggaaaa ggagcctgat aatgaaaggg
    17221 tttgtttgac attgttctct cacttactgc ctggtttggt tatgctctgg gcgggtgttc
    17281 cgttgccctg ttaaaagcga gtaacaatat cctacacact tttttaacaa aaactgagac
    17341 tagtacgact ttttgcggct ccaggttact tcccgtagga ttcttgcttt aatagcggga
    17401 ttaatttcca cattaaaaca gggattgatc atgcaaaaac tcattaactc agtgcaaaac
    17461 tatgcctggg gcagcaaaac ggcgttgact gaactttatg gtatggaaaa tccgtccagc
    17521 cagccgatgg ccgagctgtg gatgggcgca catccgaaaa gcagttcacg agtgcagaat
    17581 gccgccggag atatcgtttc actgcgtgat gtgattgaga gtgataaatc gactctgctc
    17641 ggagaggccg ttgccaaacg ctttggcgaa ctgcctttcc tgttcaaagt attatgcgca
    17701 gcacagccac tctccattca ggttcatcca aacaaacaca agtctgaaat cggttttgcc
    17761 aaagaaaatg ccgcaggtat cccgatggat gccgccgagc gtaactataa agatcctaac
    17821 cacaagccgg agctggtttt tgcgctgacg cctttccttg cgatgaacgc gtttcgtgaa
    17881 ttttccgaga ttgtctccct actccagccg gtcgcaggtg cacatccggc gattgctcac
    17941 tttttacaac agcctgatgc cgaacgttta agcgaactgt tcgccagcct gttgaatatg
    18001 cagggtgaag aaaaatcccg cgcgctggcg attttaaaat cggcgctcga cagtcagcag
    18061 ggtgaaccgt ggcaaacgat tcgtttaatt tctgaatttt acccggaaga cagcggcctg
    18121 ttctctccgc tgttgctgaa tgtggtgaaa ttgaatcctg gcgaagcgat gttcctgttc
    18181 gctgaaacac cgcacgctta cctgcaaggc gtggcgctgg aagtgatggc aaactccgat
    18241 aacgtgctgc gtgcgggtct gacgcctaaa tacattgata ttccggaact ggttgccaat
    18301 gtgaagttcg aagccaaacc ggctaaccag ttgttgaccc agccggtgaa acaaggcgca
    18361 gaactggact tcccgattcc tgtggatgat tttgccttct cgctgcatga ccttagtgat
    18421 aaagaaacca ccattagcca gcagagtgcc gccattttgt tctgtgtcga aggcgatgca
    18481 acgctgtgta aaggttctca gcagttacag cttaaaccgg gtgaatcagc gtttattgcc
    18541 gccaacgaat caccggtgac tgttaaaggc cacggccgtt tagcgcgtgt ttacaacaag
    18601 ctgtaagagc ttactgaaaa aattaacatc tcttgctaag cttattaaag gcttataaca
    18661 ccttcaggcg gccagtccgc ctgatttcat tttatggata atcattatga ataaatcgct
    18721 ggtagcggta ggcgtcattg ttgcgctagg cgtagtctgg acaggcggcg catggtatac
    18781 aggcaagaag attgaaaccc atctcgaaga gatggtcgcg caggcgaacg cgcaactcaa
    18841 actgaccgct cctgaatcca acctggaagt gagttatcaa aactatcatc gcggcgtatt
    18901 cagcagtcag ctgcaactgt tggtgaaacc cattgccggg aaagaaaatc cgtggattaa
    18961 aagcggtcag agcgttatct tcaacgaatc ggttgatcat ggtcccttcc cccttgccca
    19021 gcttaaaaaa ctgaacctga tcccgtcgat ggcatcaatt caaaccacgc tggtcaataa
    19081 cgaagtaagc aagccactgt ttgatatggc aaaaggtgaa acgccttttg agattaactc
    19141 ccgcattggt tacagcggtg attccagttc cgatatttcg ctcaagccgc tgaattacga
    19201 gcaaaaggat gaaaaagtcg cctttagcgg cggcgagttc cagttaaatg cggacagaga
    19261 cggcaaagct atctcccttt ccggggaggc gcaaagtggt cggatagacg cggttaacga
    19321 atacaaccag aaagtacaat tgacctttaa taatctgaaa accgacggtt ccagcacgct
    19381 ggcaagtttt ggtgagcgcg taggaaacca aaaactgtca ctggaaaaaa tgaccatttc
    19441 agtggaaggc aaagaactgg cactgctgga aggcatggag atcagcggta aatcggatct
    19501 ggtcaatgac ggtaaaacga tcaatagcca actggattac tcgctaaaca gcctgaaggt
    19561 acagaatcag gatctgggca gcggcaagct gactttaaaa gtcggccaaa ttgatggcga
    19621 agcctggcat cagtttagcc agcaatataa cgcgcaaact caggcgctgc tggcacagcc
    19681 agaaattgcc aacaatcccg aactttatca ggagaaagtg acggaagcct tctttagcgc
    19741 cctgccgctg atgttgaaag gcgatccggt gattactatc gcgccactaa gctggaaaaa
    19801 cagtcagggt gaaagtgcac tgaatctgtc gctgttcctg aaagatccgg caacgactaa
    19861 agaagcgccg caaacgctgg cgcaggaagt agatcgttcg gttaaatctc tggatgcgaa
    19921 actgaccatt ccggtggata tggcaactga gttgatgact caggtagcga agctggaagg
    19981 ttatcaggaa gatcaagcga aaaaactggc gaaacagcaa gttgaaggtg catcagcaat
    20041 ggggcagatg ttccgtctga ccaccttgca ggacaatacc atcaccacca gcctgcaata
    20101 tgctaacggt cagataacgt taaatgggca gaaaatgcca ctggaagatt tcgttggtat
    20161 gtttgcaatg ccggcattaa acgttccggt cgtacccgct attccgcagc agtaattcac
    20221 agccaaaacg ccggaggttt ttctctccgg cgttatttct tacttcagca taaagtcata
    20281 ctgaatactc attttcacat cccagtaatt caggcgatcg cccagcgcac tgccatcgcg
    20341 cttttcccgc cctttatcgg cagcaagaaa taaattcatc ccttgtaacg gcccttcgct
    20401 gacccgatag ttcataccac ctaagatacc cgtgccttca atccgcacat ctcgtttact
    20461 ggattcatag ccgcgagtca caatcggtgc aaatgtcagt gtaaattgcg gcgttaaacg
    20521 atagttgacg cccaattgcc aggactgcat tccttcacgg ttacgatcaa gacttaatga
    20581 aaagggatag ccaatgtcgg tatccaccac ggcaccattc attgcatggc gcaaataacc
    20641 accactgcca aatacgcttc cccacggcgc attccaggtt aattgtccac taaccaacgc
    20701 cgtttcattg ggctggctgg tattacgact taaaccttcg agttgcgcgt aaaagccgag
    20761 caactcccct ttcaacgtat aattcgggtc gagcggtata cttttctcga gaatactttg
    20821 tatctgattg gaaacatctt tctgtaccga tgcccccaga gaatagcgac tggtatcatc
    20881 atgccaactg gcggctaaaa aagaacgtgc gcgatgttta tcgtattttc ccgtctggct
    20941 gttatacacc ccttcatcac gaataccggt tgttgaatca gtccataaaa atactcgtga
    21001 acgccacatt ccccacagat caatatttgg cgttaaagcc caataaccta atgctccttc
    21061 agaaactgtc ggtaatgtac gttgcgaact gccagccatt aacggaatac gataaggtaa
    21121 gctaccataa ctataatccg tcccgaagcg acctaattta agcaggctat tttcactggg
    21181 ttttattttt actgccccca gggcaaagcc aaaactttta tcaccatcaa gataccaccg
    21241 ggtactcata tcagcacgag cacctaattt ataaacataa tacgctccgc cttcaacgcc
    21301 aataatattg gaataataac cactattgaa atcgagcaat cctccctgtc cccatgcgta
    21361 aatatctctt cccgcgccag cactgggctt atcagccctg cgcaattcgt ttttcagtct
    21421 gatgcgtagt cccgcttcat cgtcagccag ttgcgccgcg taagcagagg taaggccaga
    21481 ggcagccgtc aggcaaataa cggccatggc cactattttt ctcatagttg cacctttg
//
LOCUS       DAZUCE010000072        21100 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZUCE010000072 DAZUCE010000000
VERSION     DAZUCE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.072"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(305..847)
                     /gene="ycfP"
                     /locus_tag="ACHY03_002241"
     CDS             complement(305..847)
                     /gene="ycfP"
                     /locus_tag="ACHY03_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFS3905464.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(870..1895)
                     /gene="nagZ"
                     /locus_tag="ACHY03_002242"
     CDS             complement(870..1895)
                     /gene="nagZ"
                     /locus_tag="ACHY03_002242"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFS3905465.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTHLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(1906..2730)
                     /gene="thiK"
                     /locus_tag="ACHY03_002243"
     CDS             complement(1906..2730)
                     /gene="thiK"
                     /locus_tag="ACHY03_002243"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFS3905466.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2711..3352)
                     /gene="lpoB"
                     /locus_tag="ACHY03_002244"
     CDS             complement(2711..3352)
                     /gene="lpoB"
                     /locus_tag="ACHY03_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFS3905467.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3366..3743)
                     /locus_tag="ACHY03_002245"
     CDS             complement(3366..3743)
                     /locus_tag="ACHY03_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFS3905468.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3746..4105)
                     /gene="hinT"
                     /locus_tag="ACHY03_002246"
     CDS             complement(3746..4105)
                     /gene="hinT"
                     /locus_tag="ACHY03_002246"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFS3905469.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4439..6628
                     /gene="fhuE"
                     /locus_tag="ACHY03_002247"
                     /pseudo
     CDS             4439..6628
                     /gene="fhuE"
                     /locus_tag="ACHY03_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(6688..8121)
                     /gene="ptsG"
                     /locus_tag="ACHY03_002248"
     CDS             complement(6688..8121)
                     /gene="ptsG"
                     /locus_tag="ACHY03_002248"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFS3905470.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8416..9213)
                     /locus_tag="ACHY03_002249"
     CDS             complement(8416..9213)
                     /locus_tag="ACHY03_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS3905471.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9224..10228)
                     /gene="holB"
                     /locus_tag="ACHY03_002250"
     CDS             complement(9224..10228)
                     /gene="holB"
                     /locus_tag="ACHY03_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFS3905472.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10225..10866)
                     /gene="tmk"
                     /locus_tag="ACHY03_002251"
     CDS             complement(10225..10866)
                     /gene="tmk"
                     /locus_tag="ACHY03_002251"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS3905473.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10856..11878)
                     /gene="yceG"
                     /locus_tag="ACHY03_002252"
     CDS             complement(10856..11878)
                     /gene="yceG"
                     /locus_tag="ACHY03_002252"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFS3905474.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11881..12690)
                     /gene="pabC"
                     /locus_tag="ACHY03_002253"
     CDS             complement(11881..12690)
                     /gene="pabC"
                     /locus_tag="ACHY03_002253"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFS3905475.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12810..14051)
                     /gene="fabF"
                     /locus_tag="ACHY03_002254"
     CDS             complement(12810..14051)
                     /gene="fabF"
                     /locus_tag="ACHY03_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFS3905476.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14139..14375)
                     /gene="acpP"
                     /locus_tag="ACHY03_002255"
     CDS             complement(14139..14375)
                     /gene="acpP"
                     /locus_tag="ACHY03_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS3905477.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14586..15320)
                     /gene="fabG"
                     /locus_tag="ACHY03_002256"
     CDS             complement(14586..15320)
                     /gene="fabG"
                     /locus_tag="ACHY03_002256"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFS3905478.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15333..16262)
                     /gene="fabD"
                     /locus_tag="ACHY03_002257"
     CDS             complement(15333..16262)
                     /gene="fabD"
                     /locus_tag="ACHY03_002257"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS3905479.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16278..17231)
                     /gene="fabH"
                     /locus_tag="ACHY03_002258"
     CDS             complement(16278..17231)
                     /gene="fabH"
                     /locus_tag="ACHY03_002258"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFS3905480.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17299..18369)
                     /gene="plsX"
                     /locus_tag="ACHY03_002259"
     CDS             complement(17299..18369)
                     /gene="plsX"
                     /locus_tag="ACHY03_002259"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFS3905481.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18450..18623)
                     /gene="rpmF"
                     /locus_tag="ACHY03_002260"
     CDS             complement(18450..18623)
                     /gene="rpmF"
                     /locus_tag="ACHY03_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFS3905482.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18675..19196)
                     /gene="yceD"
                     /locus_tag="ACHY03_002261"
     CDS             complement(18675..19196)
                     /gene="yceD"
                     /locus_tag="ACHY03_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFS3905483.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19395..19979
                     /gene="yceF"
                     /locus_tag="ACHY03_002262"
     CDS             19395..19979
                     /gene="yceF"
                     /locus_tag="ACHY03_002262"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFS3905484.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20091..21050)
                     /gene="rluC"
                     /locus_tag="ACHY03_002263"
     CDS             complement(20091..21050)
                     /gene="rluC"
                     /locus_tag="ACHY03_002263"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFS3905485.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
BASE COUNT         5202 a         5736 c         5084 g         5078 t
ORIGIN      
        1 aatgttcttc gctattgaga ataacatgaa tggtgcattg gtcataccaa aattgatgtt
       61 aatcaagttt tgttgctgaa aagataggcg actttaccgg gtgagatcaa aaaacagagt
      121 ttattgttta ttatttgagc gagtttgtga gagtgatata gagtgagggg tgttcggtag
      181 gcctgataag aggcgcatca ggcattgatg ctcgactgtc ggatgcggcg taaacgcctt
      241 atccgaccca caggatccgg agcgcatcag gtaccggcaa ggttacctga tgcgacgagg
      301 gcatttaccc gagggttttg aacgctttaa tgcgctgtaa atgcggggag atattcttga
      361 atttgtgcgt ctgctcttcg tcccagacaa tctcgtaata atgatgcaac tcttcagatg
      421 tccgctggct gttaagcgct tcatcattac gcgacaaaat caccaggcaa cgatcgcgat
      481 tcttctcgcg gaagttggtc acacacttag tggcaatatc ggcatactct tccgggcgat
      541 caatcttgcc ttccatgttc tcataaggga acaaattagg gttgaagatc acctggcgga
      601 tatcgcaaag aaaaccaatc cgttccgccc agtatccgcc cagaccaacg ccgcaaatca
      661 gcggacgctc gtcgacgttc agttgcaaca ttttgtccac ttctttgagc agatgctgca
      721 tatcatgttt cggatgccgc gtactgtagc ttatcaagcg tacatccggg tcaataaact
      781 gcaattgtaa gactttctcg tggttacccg gactgttaga gtcaaaaccg tgtaaataga
      841 taatcatcgc ttcctcacat aagccagggt tagtgacctg ctttctcttc ctgccagcgt
      901 tcatgtaact gattcagacg ggcgctgatc gctttccagc gagccgagtc catcagttcc
      961 tgtcgcgaaa atgaaccttt atgatacaaa tgtgtaacac gttctgcctt gatcggtgac
     1021 agattatcta acacgctgac ggccccttta cgattattgc agaccaggat catatcgcaa
     1081 cccgcatcca gtgatgcctg cccgcgttcg gcataactgc ccataatcgc ggcaccttcc
     1141 atcgataaat cgtcagagaa aatcacgccg tcaaaaccca gttcctgacg caaaacggtt
     1201 ttcagccagt aggaagaacc gctcgccgga cgcggatcaa catcactgta gatcacatgc
     1261 gcaggcataa tggcgtcgag tttattttcg cggattaacg tgctgaaaac cgacatatct
     1321 ttggcacgaa tttccgcttg cgggcgtggg tcgcacggcg tctctttgtg tgaatctgca
     1381 gttactgcac cgtgtcctgg gaagtgtttc ccggtcgttt tcattccggc ttcatgcatc
     1441 ccatcaataa accgactggc gattgccagg gctttttctg ggtcggcatg ataagaacgc
     1501 tcgccaatcg ccgcgctgat atgtcctaca tccagcactg gcgcaaagct gatatcaata
     1561 tccatagcga tcatttcgct ggccatcagc caacccgcct cttgcgccag tttgccgccc
     1621 tcttccattc ccaacagcgc agcaaaggat tgtgctgccg gtaagcgggt aaaaccttcg
     1681 cgaaaacgct ggacgcgtcc accttcctga tctaccgcca ccaccagatg attgcgcgat
     1741 gctgcgcgga tctggcgcac cagttcacgt aactgggcag gatcatgata gttacgcgta
     1801 aagagaatca gccctcccac cagcggatgc gccagtattt cacgctcttc cgcgtccagt
     1861 tcgtaacctt cgacatccaa cattactgga cccacactgc tctccttatt gttttattaa
     1921 tagctgccgc caggtgtcat cggccagcct gataaattgt tgatcgccgg tttgtcgcca
     1981 gcggtactca aaccaccctg ctttgagcat cagcagccag ggaaaccatc gcctgacctg
     2041 acgccataat tgcgccggat aaatcttcgc gcgagtggca tagtcattga ccaattgccg
     2101 gtgctgttca gtattttcca cccacaccgc cgccagttcc agcgcgatat caccatctcc
     2161 ggcatactcc cagtcgatga gttttaaccc tgacgcgcta tgcactaaat ttccggcgtg
     2221 gacatccata tgcaatggac ttaagcgtaa aggccgtggt tcccgcgctt tgcgcagacg
     2281 ttttaacatt cgcagccaac ccactgtccg ccgcgccgga tcgctttgct gccagtacag
     2341 ttccagtaac ggcaacagcg ttattcgcca gccaaaacgt ggttgttgat gtagataata
     2401 cagcaagcct gccagttcgt tggtatccgg caaatacgtt tttaccgcgc cgggcagata
     2461 gtcgactacc atccagtcac ggagatataa atgcggcttc ggtgcaatgc atgcgggtag
     2521 ttgtgataaa gcccgatact ggcgcaagaa cgcggactgc ggcgcatcag gatcgtgcgg
     2581 ctgacgcaca acaaaacgct ggccctggtg ttcaatgaga aaactcccgc cactaagccc
     2641 actattaaac gtcgtgacgg gatgaaactg cgggaaaaag cgcgacagca attcgtcgcg
     2701 cgtgatggga ttattgctgc gaaacggcac ctttacctga ccagataatt tcgcccgtct
     2761 gcaccagcat cagctgcatt tgtagggtcg gagcgttaac gttgccagag gcgctggagt
     2821 acagcacgta atgagcgccg acattgcggg caatgcctat ggctttacta cgggtaccta
     2881 aactgtcctg cggcgacaaa cctaactgtt gcttagccat cgacagctgc tgggcggaaa
     2941 ccagggtaaa tttaccgtta ttagccagcg catttcgcag cgtttcggtc gcttctgcgg
     3001 cattcagcga accgttagta cggttgttaa cgctatcaac cagaaggacg ctacccgcag
     3061 tcaccccgtc agccccaagc atcttactga ccatcggctg cattgcgcca ttccagtcat
     3121 aatggcgaat atgcggcgca ggcggtgcag tttgatcttc gtgctcaatt gggcctggct
     3181 gctgcgggat cgtcggcacc gagggcactg tggggacagg ctgttgtggc tcggctggtt
     3241 gttccggcgc tggtttcact tcttctaccg gtgcaggttc acgttgcccc acacacccgg
     3301 cgagaaacat cgccagcgcg gtaatcaagg cgtagcgact catttttgtc atcaagattc
     3361 accccttaca aatatagata aagtctgact ttgtgcgccc ccagaaaatt ggcgctgccg
     3421 tacagcgtta ccgccgaatg tgcgggaatg gtaacgctgc gtggcctttc cagaggatgc
     3481 atctccagcc ctctggcgtc ataccagtaa aaacgataat gaacggtaac gggttcttgc
     3541 ctttcgttat aaagcgttga ggatgctgaa ggttgaatat cagacgtcga aaggacgggc
     3601 ttttctgcac tgatgcccgc agccaataaa gatgactcca tcaccagcga ttgctcatca
     3661 ttcaccggaa tttccggatg tgaacgacag cccaccagca gtaacaacgc cagagacacc
     3721 agcccaaagc atccttttct catcgttaca gacctttatg cgccagcatt ggtcccagcg
     3781 gacggccacc caacaagtgc atatggatgt ggtaaacctc ttgtccgcca tggcggttgg
     3841 tattcatgat cagacgatag ccatcttcgg caataccttc ttgctcagca atttttgccg
     3901 ctacggtgat catgcgtccc agcgcctgct catgctcagc tgacacgtcg ttcacagtcg
     3961 gaatgaggat attcggaatg atcagaatat gcgttggcgc ttgcggcgaa atatcgcgaa
     4021 acgccgttac cagatcatcc tggtagacga tatcggaggg gatctcacga cgaataattt
     4081 tgctgaatat agtttcttct gccacgacat tttccttttt caaatagcca tgcgttgcgc
     4141 catgctacgc cgcgccacac tgcgagtata gagtatggtc gagttaaacc tgctctttca
     4201 accttaactc acgatttctt aagcaaaaaa tcgttttgct gccgtatata tcgccattat
     4261 tcccatttct gcctgcgaat gcgctgaaaa gacattctct gtttggttta cacgtcaaat
     4321 atgaatgcgt atatttctca tttgcattta catacaaaat tattcgcaca ctaaatataa
     4381 ccgctcatca ccctcctccg gcatgagcct gacgacaaca taaaccaaga gatttcaaat
     4441 gctttcaaca caatttaaca gggataatca atatcaagcc atcaccaaac cgtcactact
     4501 tgccggttgc atagcattgg cactattacc ttctgccgct tttgctgcac cagccactga
     4561 agaaacggtg attgttgagg gttcagccac agctccagat gatggcgaaa atgattacag
     4621 cgtaacgtct acctctgcgg gtaccaaaat gcagatgact caacgtgata ttcctcagtc
     4681 ggtcactatt gttagccagc agcggatgga agatcagcag ttacaaacgc tgggcgaagt
     4741 gatggaaaac acgctgggga tcagcaaaag tcaggcggat tccgatcgtg ctctttatta
     4801 ttcccgcgga ttccagatcg ataactatat ggttgatggt atccccacct attttgaatc
     4861 gcgctggaat ctgggcgacg cactttctga tatggcactg tttgaacgcg tagaagtagt
     4921 gcgtggcgcg acaggactca tgaccgggac gggtaatcca tctgcggcaa ttaatatggt
     4981 tcgaaaacac gcgaccagtc gtgaatttaa aggcgatgtc tcggcggaat acggtagctg
     5041 gaacaaagaa cggtatgtgg cggatttaca aagcccactc accgaagacg gtaaaatccg
     5101 cgcgcgaatt gtcggcggct accagaataa cgactcatgg ctggaccgct acaatagtga
     5161 aaagaccttc ttctccggca ttgtcgatgc tgatttaggc gatcttacga cgctttcagc
     5221 cggttacgaa tatcagcgca ttgatgttaa tagcccaacc tggggcggtt taccgcgctg
     5281 gaatactgat ggcagcagca acagttacga tcgcgcacgc agtaccgcac ctgactgggc
     5341 gtacaacgat aaagagatca acaaggtctt tatgaccctg aagcagcggt ttgctgatac
     5401 ctagcaagcg acactgaatg ccacccactc tgaaatcgaa tttgacagca aaatgatgta
     5461 tgtcgatgcc tatgtaaaca aagcggatgg tatgctggtt gggccataca gtaattatgg
     5521 acctggcttt gattatgtcg gcggcaccgg ttggaacagt ggcaaacgta aagttgatgc
     5581 gctggatttg ttcgctgacg gtagttatga attgtttggt cgtcagcaca atctaatgtt
     5641 tggtggcagt tacagcaaac aaaacaatcg ttacttcagt tcatgggcca acatcttccc
     5701 ggatgaaatt ggcagtttct acaactttaa tggcaatttc ccacaaaccg actggtcacc
     5761 acagagcctg gcgcaggacg ataccacaca tatgaaatcg ttatatgctg ccactcgtgt
     5821 cacccttgcc gatccgctgc atctgatcct cggcgcacgt tataccaact ggcgggttga
     5881 tacgctgact tacagcatgg agaaaaacca caccacgcct tacgctggtc tggtgtttga
     5941 catcaatgac aactggtcga cctacgccag ctatacctct attttccagc cgcaaaatga
     6001 tcgtgacagt tcaggcaaat atctggctcc aatcaccggt aacaactacg agctgggtct
     6061 gaaatcggac tggatgaata gccgtctgac caccacgtta gccatcttcc gtattgagca
     6121 ggataatgtc gctcagtcca ccggtacacc tatccccggc agcaacggcg aaaccgccta
     6181 taaagcggtg gatgggacag tcagtaaagg ggtggaattt gaactcaacg gcgcaattac
     6241 cgacaactgg cagctgacat ttggcgcaac gcgctatatt gcagagtata acgaaggaaa
     6301 cgccgttaat cctaatctgc ctcgcaccac ggttaaaatg ttcaccagct accggttgcc
     6361 tgtcatgcca gagttgacgg tcggcggtgg tgttaactgg caaaatcgcg tgtataccga
     6421 caccgtgaca ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc tggtggatct
     6481 cttcacccgc taccaggtga cgaaaaactt ctcgttacag gggaacgtca ataacctgtt
     6541 cgacaaaacc tacgatacca acgtggaagg ttctatcgtc tacggcgcac cgcgtaattt
     6601 cagcattacc ggcacgtatc aattctgaat aacacctgta aaaaaggcag ccatctggct
     6661 gccttagtct ccccaacgtc ttacagatta gtggttacgg atgtactcat ccatctcggt
     6721 tttcaggttg tcggatttag taccgaaaat cgcctgaaca ccagaaccag caaccactac
     6781 gcccgctgcg cccagtttct tcaggccagc ctgatccact ttagacacat cagcaacgct
     6841 gacgcgcaga cgggtaatac atgcgtcgag gttagtaatg ttttctttac caccaaatgc
     6901 agcaaccaga gccggtgcca tttcgctggt acctgtcgct tttgcatctt cagtcgcgtc
     6961 ttcacgaccc ggcgttttca gatccagtgc tttaatcagc acgcggaaga tggtgtagta
     7021 aacaatcgca taaccgatac cgacgatcgg gaacagccac agtttgctgc tgttaccaga
     7081 cagaacgatg aagtcgatca gaccgtgcga gaacgacgta ccgtcacgca tccccagaag
     7141 aatacagatt gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca ggatcggcgc
     7201 aacgaacatg aaggagaact cgatcggctc ggtgataccg gtcaggaacg aggtcagcgc
     7261 cgcggagatc ataataccgc ccactttcgc gcggttttct ggtttagcag agtgccagat
     7321 agcaattgcg gcagctggca gaccgtacat tttgaacagg aagccaccag acagtttacc
     7381 cgcagtcggg tcacccgcca tataacgcgg aatgtcgccg tggaaaacct gacctgctgc
     7441 gttggtgtat tcaccaatct gcatctggaa aggtacgttc cagatgtggt gcagaccaaa
     7501 cggtaccagg caacgttcga tgaaaccgta aatgccaaac gcaactaccg ggttctggta
     7561 agcagcccac tgagagaagg tctggattgc agaaccaatc ggcggccaaa tgaaggacag
     7621 cacaacgcca gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc gtttaccggc
     7681 aaagaagcca agatactcag gcagcttaat acggtagaaa cggttaaaca tgtacgctgc
     7741 gatcgcaccg gagataatcc ctccgagtac gccagtatcc gccaggtgtt tagaggcgat
     7801 ttcttcagca ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt taaccatgat
     7861 gccataggca acaactgcgg ccagcgcgga tacgccatcg ttattggtaa agccgagggc
     7921 gacaccgatc gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg cttctgccat
     7981 aacatgcgat acaacggcgg gcagccagct gaaattcgcg gaaccgacgc ccagcagaat
     8041 acctgcgata ggcagtacgg ataccggcag catcagcgat ttaccgacct tttgcaggtt
     8101 agcaaatgca ttcttaaaca taattgagag tgctcctgag tatgggtgct ttttttacgt
     8161 tctcacgcgt ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag cgccctttat
     8221 ttattacaca gagtaaaata attcagtgcc aatttgtttg acggctatca cgtttcaact
     8281 tcagatcatt ggagaagttg cacattttga caaaacatat gtcacaatat ttcaaacgtg
     8341 gttattcacc gcccaatttt cggcggtgaa ctttactcgt tttagccatt aattacgagc
     8401 tttaaaaaaa ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt gaaacagacg
     8461 ggcgaagtta tcggtggtta cctgcgccag ttcttcaacg gcaacacctt tcaacacagc
     8521 catgtattct gcaacgtcac gaaccatcgc aggttgattc tcttttcctc gatgcggtac
     8581 cggcgcaagg taaggtgagt cagtttccac cagtaatcga tccaggggga cataacgcgc
     8641 agcatcgcgc agttgctccg cattacggaa ggtcacaatg ccggaaaagg agatgtaaaa
     8701 tccgagatcc agtaatttac ccgccgtttc tctgtcctct gtaaaacagt gtagtacgcc
     8761 accgcaatcc gtcacttttt cttcgcgcag aattgccagc gtatcggcac gggcgtcacg
     8821 ggtatggacg ataaccggct tgttcagttc acgaccaatc tggatatgat ggatgaagga
     8881 ctcttgctga cgtactttag tttccggtgt gtaataataa tccagcccgg tttcacccag
     8941 cgctacaaca ccctcttctg ccgccagacg gcgtaaatct tctacatcgt agggatcatt
     9001 ctggttgagt ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc ctaccagatc
     9061 ccgcatatgt aaataacccg gtaatgttgt ggcgactgcc agacaaaatt tcacatcgcg
     9121 tgcggcggct ttcgccagaa cgtcatccac gtccttatgc aaagattcat aatccagacc
     9181 atcgagatgg cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga tgaggaaccg
     9241 gtagcacaac gcccggttgc aggtaatgct caatacgcag taaaagatcg gtgatgagaa
     9301 gctcgcggtt gatgcctgta acagacatta actgttcacg aatgtggcaa acatccccca
     9361 gtatagcctg caggcgcgag ggagaaagat ggtttgccag ttcggcgacc aggcccggca
     9421 catcaacatt ggtcacctgc gcagcaccat gatggcgttt tagcgcatcc atcagcaacg
     9481 ttgccagcca gtgtaaacgc gccggagctt gttcatgatt aagggccgct agcagcgaat
     9541 accagtcgcc cgatggcacg ctatatgcca acgcctgaca caatgtttca cgagcctgcc
     9601 agttatctcc ctgaaacaac gccagtgccg cgccaggcga accggcgctt aagcgcaatg
     9661 cggcaagtaa tgcatcctgt gacattgtca cttcgcgtga aagccaggtc acggcgtact
     9721 gttccggcgg cggcgcaagg taatgtaacc gacaacgact acgtaatgtt gccagtaaac
     9781 gttcaggctc gcgggtagcc aggaaaaacc aggtttctgc tggtggctct tcaagcgttt
     9841 tcagcaatgc gttagccgcg gcgtcggtta gtaaggcagc atcggttacc cagacaactt
     9901 tcgcaccacc taagcgtgcg tgctcattca gcttttcggt gacctcacgt accgcatcaa
     9961 tgcccaacgc atttttccct ttttcgggag ccagggtgta gtaatcggga tgcgtgccag
    10021 cctgcatcaa ctgacatcca cgacagtgac cgcaactttt gtggccctgc ggttgttggc
    10081 agagtaaata acggctcagg gcgtagatta aagcatcatc gcccatgccc ggtaacgcct
    10141 gaatgagtag cgcatggtga cctcttccgg cctgatagct ggctaccagt ttttcgaaat
    10201 caggtcgtaa ccatggatac catctcatgc gtccaactcc ttaacccagt gggtcacggt
    10261 agtgcggatt gcatccatca cggcctccag cggctgggtg gcatcaatgg tatgaatact
    10321 tttatcttgt gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga aatcgaaaga
    10381 ctcttgctca atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc caacttccgg
    10441 ggtaacatcg agatagagcg ttaagtcggg gcgaaaatcc ccgagaacag catcacgcag
    10501 tgttgccagc atatgttggt caataccacg tcctccgccc tgatacgcct gagtggagag
    10561 atcgtggcga tcgccaatca cccaggtgcc gttagccaga gctggtttga tgaccgtttc
    10621 taccagttga acgcgcgcgg cataaaacat cagaacttcg gctttatcgg taatgacttc
    10681 atcgcctacc gatttgatat ccagcaccag gcttcttaac ttttcggcaa gttgcgtacc
    10741 gccaggttcc cgagtgaaaa ccatgtcgcg gatacccagt tgctcaagcg tctcaaccac
    10801 cacattacgc gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga tatacttact
    10861 gcgcattttt ttccttaagc actttcagat aatcctgcac agacttgtta tggctggcaa
    10921 gattggtatt aaacgtgtga ccacctttac catcggccac aaaatagaga tacggcgttt
    10981 ttgccggatg cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc gcacccggcg
    11041 gcaaaccggt aatggtatag gtgttatacg ctgtcggcgt ttccaggtct gcacgagaaa
    11101 gtttgccatt ataacgctct cccatcccgt aaatcacggt tgggtcggtc tgcaggcgca
    11161 taccaatgcg taaacggttg ataaataccg aggcaacctg atcgcgttca ctggcaacgg
    11221 cggtttcttt ttcgataatt gatgccatcg tcaccagctg atttttatct ttataaggca
    11281 gaccgtccgc acgcccttcc caggcgctat cgaccgcttt caccattttc ttgtgcgctc
    11341 gcttgagtaa cgcgacatcg gtggtattgg cggtatacat ccaggtgtct ggccagaacc
    11401 aaccttcaat ccactccggg ttttccagtt caagtgcctg cgctacggtg gcgtacttat
    11461 cgtcactcag cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg tagtcgctca
    11521 gacgcatccc ttctaccagt cgcagcggga actgtgcttc tttaccgctt tccaacaatt
    11581 tcagcatctc gcgcacggtc atctgcggtg taaagcggta agtcccggct ttaaagtgag
    11641 aaagatccgg ttcgatacgc agcagccatt gaaaaacccg tgggcgattg atgatcttat
    11701 cagcataaag ctgttcaccg agcgccagac gtccggtccc tggcttcagg gtaaatatcg
    11761 tctcttcttt gataagcaat ttgctgtcgg caagatggcg aaccttccag acgcccacac
    11821 cagcggcgat acccagtacc accagcaata acaagattat caataacact tttttcatga
    11881 ctaattcggg cgctcacaaa gtggggctaa atattcatat aacgttgctg acgaaaagga
    11941 gacatcgcca caggcacgta cgggcatcac tggcattaac gcattacaaa taaccatctc
    12001 atctgcctgc aacgcctctt ccagagaagc ttgcacttcg acaagctgat aagaggattg
    12061 tgccagcaaa cggatacaga attgtcgcat aatgccgtta acacctgcct gatccagtcg
    12121 cggcgtataa actacgttgc ccttccgcca gaacaaatta gccgcacagc attccgtaac
    12181 ccacccttcg ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa gatgagagcg
    12241 aatcaatact tgctcaagac gattgagatg tttaatacct gcaagatgag gattgcgccc
    12301 cagccgcacc gggcttagcg ccaacgtcat cccctcgtta cgcaaacggt cgtaatgtgc
    12361 aggataagcc gtaacggaga gaatccgcgt tgccggtcca ctgttcaatg tgctgtaccc
    12421 tcgcccgcca ctaccgcgac tgatcacgac tttcagtaca ccattttgct gttctgctgc
    12481 cagcgttttc atctcttgtt caagctgtgg ccagaagtca caggaaatca tcaaccgctg
    12541 acaagcatcc tgtagtcgct ggatatgcgc cgataacaaa ctgactttac cgtcgataac
    12601 tctggcggtg gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta ctgccagcga
    12661 ttcctgctta taaccgttaa ttaagaacat accggctcct tatcagagaa ttgttagtgt
    12721 ggcagcatgt tcactacgga acaagtcgga ataaaagcta agaaaaaagg cccgcaagcg
    12781 gaccttttat aagggtggaa aatgacaact tagatctttt taaagatcaa agaaccatta
    12841 gtgccaccga agccgaagga gttacacagc gtgtattcca ttccgctgac ctgacgtgct
    12901 tcatgcggta cgaaatccag atcgcaacct tcatccgggt tatccaggtt gatggttggc
    12961 ggaacagcct gatcgcgcag cgccaggatg gagtagatag attctactgc acccgccgca
    13021 cctaacagat gaccggtcat agatttcgtg gagcttacca acacacggct tgcagcttca
    13081 ccgaagatgg ttttcaccgc ctgcgcttca gctttatcgc cagccggcgt agaagtaccg
    13141 tgcgcgttaa cgtagccaat ctgactcgct tcaatgcctg catcacgcag agcatttgcc
    13201 attgccagag ctgcgcctgc gccattttct ggcggtgacg tcatatgata agcatcgctg
    13261 ctcataccaa agccgacgag ttcagcgtaa attttcgcac cgcgtttttt cgcgtgttcg
    13321 tactcttcaa gtaccagcat accggcacca tcgcccagta cgaagccatc acgctcttta
    13381 tcccacgggc ggctcgccgc ttgcgggtta tcattgcggg tagataatgc acgtgccgcg
    13441 ccaaaaccac caacacccag cggcgtactg gctttctctg cgccacctgc aaccatcacg
    13501 tcagcatcgc catacgcgat aatacgcgca gcatgaccaa tgttgtgcac gccggaagta
    13561 caggcggtcg cgatagagat gcttgggcca cgcaggccat acatgatagt cagatgacct
    13621 gccaccatgt tcacaatcgt tgacggaacg aagaatgggc tgatcttacg tggaccaccg
    13681 ttcatcagag atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc ggagccaatt
    13741 gcggcaccaa tgcgggttgc gttctcttcc gttatttcaa ggccagaatc ctgcatggcc
    13801 tgaacgccag cgacaattcc atattgaatg aaggcatcca tcttgcgctg ttctttgcgc
    13861 gagataatgt cctcacagtt aaaatccttt actaagccag caaatttcgt tgcataggcg
    13921 ctagtatcga aatggtcgat taggctgatg ccactctgac cggcaagcag agctttccag
    13981 gtagactcta cggtattgcc gacaggagac aacatgccca gtccggtcac aactacacga
    14041 cgcttagaca cgtttgtcct ccagggaggg aaaaaatgat tctagtggga caaaaagata
    14101 aaactcaggc ggtcgaacga ccgcctggag atgttcactt acgcctggtg gccgttgatg
    14161 taatcaatgg cagcctgaac ggtggtgatt ttctcagctt cttcgtccgg aatctcagta
    14221 tcaaactctt cttccagagc cattaccagc tcaacggtgt caagagaatc cgcgcccagg
    14281 tcttcaacga aagaagcatt gttggtaact tcttcctgct taacgcccag ctgttcgccg
    14341 ataattttct taacgcgttc ttcgatagtg ctcatactct taaatttcct atcaaaactc
    14401 gctttcgcga tggttttcgt agtgtataaa atgttgaaaa atttgcaact aaatcccggc
    14461 aggtcttacc acgattttac gttattttga ggccttaccc cccaataacg caaatcattt
    14521 tgcactaatt attactcata accacgcaga ctgcgcgcaa catgagcatt ttgtgcaaat
    14581 cgcggtcaga ccatgtacat cccgccgttc acatgcaaag tttcacccgt gatgtaagct
    14641 gcttcgtcgg atgccaggaa tgcaaccgcg ttggcgattt cctgtgcgcc gccgaggcga
    14701 cccgcaggaa cctgcgccag gatacccgca cgctggtcat cgctcagcgc acgtgtcatg
    14761 tccgtttcaa taaagcccgg agcaacaacg tttacagtaa taccgcgtga cgcaacttcg
    14821 cgcgccagtg atttactgaa gccgatcaag cccgctttcg ccgcagcgta gttggcctga
    14881 ccgccatttc ccatggtacc aaccacagaa ccgatagtga taatacgacc atgacgcttt
    14941 ttcatcatag cgcgcattac cgcttttgac agacggaaaa cagatgaaag gttggtttcg
    15001 ataatatcgt tccactcttc atctttcatt cgcattaaca ggttatcacg agtgataccg
    15061 gcattattga ccaggatatc cacttcacca aattctgcgc gaattttttc cagaacagat
    15121 tcgatagatg ccgggtcggt cacattcaac atcagacctt tgccgttagc acctaaataa
    15181 tcactgatcg cctgagcgcc attttcactg gtcgcagtgc caataacttt cgcgccacgg
    15241 gctgcgagcg tttcagcaat tgcgcggcca attccgcggc ttgcaccggt taccagtgcg
    15301 atttttcctt caaaattcat gattttcctc ttttaaagct cgagcgccgc tgccatcgct
    15361 gaaggttcgt tcagcgccga ggcggtcagg gtgtcgacaa tgcgtttcgt caggccagtt
    15421 agcactttgc ccgggccgac ttcatagaga tgttctacgc cttgcgctgc catgtactca
    15481 acagacttcg tccactgaac cgggttatac aactgacgta ccagtgcgtc acgaatggca
    15541 tcaccattgg tttcgcattt cacatcaacg ttattcacaa caggaactgt tggtgcgtta
    15601 aaggtgattt tcgctaattc tactgccagt ttgtcggctg ctggtttcat cagcgcacag
    15661 tgagacggta cgctcactgg taacggcagc gcgcgttttg cgcccgcagc tttacaggca
    15721 gcgccagcac gctcaaccgc ttctttatga ccggcaataa ccacctgtcc cggagagtta
    15781 aagtttaccg gagaaacgac ctgaccttct gcagcttctt cacacgcttt cgcaatagac
    15841 gcatcatcca gaccgatgat tgccgccata gcgccagtgc cttccggtac ggcttcttgc
    15901 atgaacttac cgcgcatctc aaccagacgc accgcatcag cgaaatcaat cacgccagcg
    15961 caaaccagcg cggagtattc ccccaggctg tgaccggcca tcattgccgg tgctttaccg
    16021 ccctgctgct gccatacgcg atacagcgca acagatgcag tcaacagcgc tggctgggtt
    16081 tgccaggttt tattcagttc ttcagctggc ccctgctggg tcagcgccca caggtcgtag
    16141 cccagcgccg cagaagcttc agcaaacgtt tcttcgacaa ttggatagct cgccgccata
    16201 tcagccagca ttccaacggt ttgagaaccc tgtccaggga acacaaatgc aaattgcgtc
    16261 atgttttaat ccttatccta gaaacgaacc agcgcggagc cccaggtgaa tccaccgcca
    16321 aaggcttcaa gcagaaccaa ctgccccggc ttaatgcgcc cgtcgcgtac agcttcatcc
    16381 agcgcgcacg ggacagaggc cgcagaggta ttaccgtggc gatccagcgt caccacgaca
    16441 ttgtccatag acataccgag ttttttcgcc gttgcactga taatacgcag gttagcctga
    16501 tgcggaacca gccagtccag ttgagaacgg tcaagattat ttgccgccag cgtctcatca
    16561 acgatgtgcg ccagttccgt taccgcaacc ttgaagactt cgttgcccgc catcgtcaga
    16621 tgaattgaat tctctggatt cacacggtca gcattaggca gcgtcagcaa ctcaccatag
    16681 ctaccgtcgg catgcagatg ggtggagatg attcccggct cttcagaggc agccagcacc
    16741 gcagcgcccg cgccatcgcc aaaaataata atagtcccac gatctgttgg atcgcaggtg
    16801 cgcgccagta catcggaacc gacgaccaga gcatacttca ccgccccaga tttcacgtat
    16861 tgatcggcta cgcttaatgc ataggtgaaa cctgcgcagg ctgctgcaac gtcaaatgcc
    16921 gggcaacctt taatgcccaa catgctttga atctgacaag ctgcgctcgg gaaagcgtgc
    16981 gtagcagaag tcgttgccac aacgatcagg ccaatctggt ctttctcaat gcccgccatc
    17041 tcaattgcgc gtgtcgccgc ttcaaagccc atggttgaaa cggtttcgtt tggcgcggca
    17101 atgtggcgtt cgcggatacc ggtacgagtg acaatccact cgtcagaggt gtccaccatt
    17161 ttttccaaat cggcgtttgt ccgcacttgt tcgggcagat agctgccagt accaataatc
    17221 ttcgtataca tgtacgctca gtcacttttc ggttatatac cgtcacttgc aaactgcgag
    17281 ttcgctggca gcgtcctgct accgcagagt tccgcttttg ccaccgtcca gcagctcaaa
    17341 accagctggg tatacagatt ccaggcgagc ggcaattcgc tgaggaactt gtcgctgcac
    17401 cgcctgcact gcctgttcaa tcgcgaccgc aaaagctcgc tgattggctg caccatgact
    17461 ttttatcacc gtgccgcgca atcctaacag acaggcgccg ttatactggt cggggttgag
    17521 gtgactgaat cgcctcgtca ggctcttttg tagccaacgc tttaataaca gtagccacca
    17581 cgaccgtttt ttcccttcac cctgagattt cagcagagaa aggaacatcc tgacaacacc
    17641 ttccatcgtc tttaatgtga catttcctgt aaagccgtca caaaccagca catctgtctt
    17701 gccagttaac aactcattgg cttcaagata gccgatataa ttgatagaag ggattgtttt
    17761 aagcaccgct gaggcatccc gaatactatc gagacccttt acttcttctt caccaatatt
    17821 gagcaacgcc acgcgaggat tgggaatttc caccacctct tcagccagga ctgagcccat
    17881 aatggcaaat tgcaccaaca ttgtgctgtc acaatcgacg ttggccccta agtcaaggac
    17941 caccgttttg cccttttgct gatgtggtaa taccgtcacc agcgccggac gctcaatccc
    18001 ctccaggggc ttgagtaata attttgccag ccccatcagt gccccggtat taccggcact
    18061 gacacaggct tgcgctcgac cttctttcac cagctccagg gccacgcgca ttgaactccc
    18121 acgactggcg cggatagctt gcgaaggccg ggcatcactg gcgataactg actgcgcagg
    18181 aattatctgc agacgcgaac gttgttcaaa gtcagcttta gcaagtaatg gcgtgatggc
    18241 gtcgggattg ccgactaaaa gaagagtgag ttgcgaatta gaattcagtg cctgcaatgc
    18301 tgcaggcact gtcacggaag ggccaaaatc ccctcccatg acatctaacg ccagggttag
    18361 acgtgtcaag gtatcgtcgc cgcccggttt ggtctttccc cagttgcctg gggaaaatcc
    18421 tcactaagct tcatcacgca gacgcgtgat tacttagcga tgaccttgcg gccgcggtag
    18481 taaccgtcgg cagtgatgtg gtgacgcagg tgtttttcac cagaagtttt gtctacagac
    18541 aggctggtga ctgcggtcag cgcgtcatgg gaacgacgca tgccacgttt ggaacgggtt
    18601 ggtttattct gttgtacggc catggacctt actcctcaat tacggtttat ccccgatcca
    18661 acggggagca ccaattactt acgctttaag ctggctaata cggcaaatgg gtttggcttt
    18721 tgcgcttctt caggcagttc accaaagacc atgtccgctt cggacacttc acagtgttca
    18781 gaatcatgca ccggaactac cggcaaggcg aggatgattt catcttcaac cattgcaagc
    18841 agatcgattt caccgaattc gttaacctca atcggttcat acgcttccgg cagtgcttca
    18901 gcctgttcgt ctgaacgcac aggactaaaa caatacgttg tgtagacctg atgagtaaac
    18961 ggcttcccgc aacgctgaca ctcgagcgtt accgtcacct tcgcatcgcc gtttaacact
    19021 gcgagacgtt ggttatcgat agcgaacgac atggagcatt ccacatcact gtccacactg
    19081 actacggatt cggcgacgcg ctcaacctga tcaggggtat agataccctg gtaatcaagg
    19141 cgtttttgag ccgtacgaac cggatcgaga gtcaggggta attttacctt ttgcataggg
    19201 cgcgcatatt aactttgtaa cgtcatagag tcaaagaaaa aggcagcctg tggttgcctt
    19261 ttgccaataa ttcgcacaca ttgcgggtta ctgctttatt ttcgttcagt gctgataacg
    19321 aacatcaacc tgcaaaacga agcgcatagt ttaaaatggc tatcatcaat acgctatatc
    19381 tgatggaaaa aaatatgcct aaacttattt tagcctccac ctcgccctgg cgtcgcgctc
    19441 ttctggaaaa actgcaaatc tcttttgaat gtgcagcacc cgaggtcgac gaaactccac
    19501 acagcgacga atcaccgcga cagttggtgc ttcgactggc acaagaaaaa gcgcaatctc
    19561 tggcgtcacg ttatccggat catttaatta ttggttcaga ccaggtatgt gttcttgatg
    19621 gcgaaatcac cggtaaaccg ttaacggaag aaaatgcccg tctgcaatta cgcaaagcca
    19681 gcggcaatat cgtcaccttc tatactggac tggcgttgtt taattcggcg aatgggcatc
    19741 tacaaacaga agtggagcct tttgacgtcc atttccgtca cctgagcgag gcggagattg
    19801 ataattacgt gcgtaaagag catcccctgc actgcgcggg tagctttaag agtgaaggat
    19861 ttggcattac gctgtttgag cgtttagagg ggcgtgaccc taacacgctg gttggtttac
    19921 cgctaatcgc gctgtgtcag atgttacgtc gggaagggaa aaacccgctg atgggatagt
    19981 tttttacgcc ggatgcggtt acgtcgcatc cggcaatcgg tgctggcggc ctgatgcggc
    20041 gtaaacgcct tatcaggcca accgcccatt atcaggttta tatgcttgta ttagcgcgcg
    20101 ttacgcagct tttgcaaaca acgcttcaaa ccttcatcca tcggcgcttc gatacgcatc
    20161 acctcaccgg tccccggatg agtaaacttc aacgctgcag cgtgcaggaa caggcgattt
    20221 aatcccgtgc ctgcttcagt gagctgtctg tcaaattcac ggtcaccgta gcgatcgtca
    20281 aaggcaatcg gatgacccgc atattgtgta tgcacacgga tctgatgagt acgccctgtt
    20341 accgggctac aacgcaccag ggtggcaaat gcatagcgtt cttccacttt aaagcgtgtt
    20401 tccgacggtt tgccttcctg actgacacgc acgatacgtt cgccgctttg cagaatattt
    20461 ttcagtaacg gcgcttgaac gctcttcaca tgcgactgcc actgaccgcg caccagcgcc
    20521 aggtaatctt tttgcatccc tttttcacgt aattgctcat gcagagaacg caacgccgag
    20581 cgttttttcg ctaccagcaa cacacctgaa gtgtcccggt cgagacgatg aaccagttca
    20641 aggaaccgcg cttccgggcg caacgcccgc aaaccttcga taacgccgaa gcttaaacca
    20701 ctgccgccat gtaccgccgt accggaaggt ttattcagca ccaggatatg atcatcttca
    20761 tataagatga cgtccgccag cgccgccacc ttttgcagat gtggtgaaac cgcctcttct
    20821 tcccgctcag caacgcgaac cggtgggata cgcacctcat caccagcttc gagtttatat
    20881 tcaggcttaa tacgtttttt gttcacccgc acttcgcctt tacgcaaaat acggtaaatc
    20941 atactttttg gtacgccttt caattgggta cgcaaaaagt tatcgatacg ttgcccagct
    21001 tcgtcagcgg tgatagcaac aatttttacg gatggagtct ctgttttcat ggttggcgat
    21061 tctaattagc caacaggatt cgcgccactc atttttctat
//
LOCUS       DAZUCE010000073        20718 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZUCE010000073 DAZUCE010000000
VERSION     DAZUCE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.073"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            18..833
                     /locus_tag="ACHY03_002264"
     CDS             18..833
                     /locus_tag="ACHY03_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3905486.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPAALKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            835..975
                     /locus_tag="ACHY03_002265"
                     /pseudo
     CDS             835..975
                     /locus_tag="ACHY03_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            1077..1550
                     /gene="ysaA"
                     /locus_tag="ACHY03_002266"
     CDS             1077..1550
                     /gene="ysaA"
                     /locus_tag="ACHY03_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS3905487.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(1701..2954)
                     /gene="avtA"
                     /locus_tag="ACHY03_002267"
     CDS             complement(1701..2954)
                     /gene="avtA"
                     /locus_tag="ACHY03_002267"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFS3905488.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(3131..5161)
                     /gene="malS"
                     /locus_tag="ACHY03_002268"
     CDS             complement(3131..5161)
                     /gene="malS"
                     /locus_tag="ACHY03_002268"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFS3905489.1"
                     /translation="MKLAACFLTLLPGFAVATSWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADVIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDNAHQRGIRILFDVVMNHTGYATLTDMQEFQFGALYL
                     SGDELTKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLHDFNVLSYLSSHDT
                     RLFRDGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVVWAGQQ"
     gene            5481..6305
                     /gene="bax"
                     /locus_tag="ACHY03_002269"
     CDS             5481..6305
                     /gene="bax"
                     /locus_tag="ACHY03_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFS3905490.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(6413..7591)
                     /gene="xylR"
                     /locus_tag="ACHY03_002270"
     CDS             complement(6413..7591)
                     /gene="xylR"
                     /locus_tag="ACHY03_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFS3905491.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKHWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(7669..8850)
                     /gene="xylH"
                     /locus_tag="ACHY03_002271"
     CDS             complement(7669..8850)
                     /gene="xylH"
                     /locus_tag="ACHY03_002271"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFS3905492.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(8828..10369)
                     /gene="xylG"
                     /locus_tag="ACHY03_002272"
     CDS             complement(8828..10369)
                     /gene="xylG"
                     /locus_tag="ACHY03_002272"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFS3905493.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(10447..11439)
                     /gene="xylF"
                     /locus_tag="ACHY03_002273"
     CDS             complement(10447..11439)
                     /gene="xylF"
                     /locus_tag="ACHY03_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3905494.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            11805..13127
                     /gene="xylA"
                     /locus_tag="ACHY03_002274"
     CDS             11805..13127
                     /gene="xylA"
                     /locus_tag="ACHY03_002274"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFS3905495.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            13199..14653
                     /gene="xylB"
                     /locus_tag="ACHY03_002275"
     CDS             13199..14653
                     /gene="xylB"
                     /locus_tag="ACHY03_002275"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFS3905496.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLEVAKRDWSDVMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYVAYQPRRETFRRLYQQLLPLMA"
     gene            14822..15163
                     /gene="yiaB"
                     /locus_tag="ACHY03_002276"
     CDS             14822..15163
                     /gene="yiaB"
                     /locus_tag="ACHY03_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFS3905497.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLRQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            15209..15646
                     /gene="yiaA"
                     /locus_tag="ACHY03_002277"
     CDS             15209..15646
                     /gene="yiaA"
                     /locus_tag="ACHY03_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFS3905498.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(15688..16683)
                     /gene="wecH"
                     /locus_tag="ACHY03_002278"
     CDS             complement(15688..16683)
                     /gene="wecH"
                     /locus_tag="ACHY03_002278"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFS3905499.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            16858..17157
                     /gene="ysaB"
                     /locus_tag="ACHY03_002279"
     CDS             16858..17157
                     /gene="ysaB"
                     /locus_tag="ACHY03_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFS3905500.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            17252..18163
                     /gene="glyQ"
                     /locus_tag="ACHY03_002280"
     CDS             17252..18163
                     /gene="glyQ"
                     /locus_tag="ACHY03_002280"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFS3905501.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            18173..20242
                     /gene="glyS"
                     /locus_tag="ACHY03_002281"
     CDS             18173..20242
                     /gene="glyS"
                     /locus_tag="ACHY03_002281"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFS3905502.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            20565..20717
                     /gene="hokA"
                     /locus_tag="ACHY03_002282"
     CDS             20565..20717
                     /gene="hokA"
                     /locus_tag="ACHY03_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFS3905503.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGGYELAAFL
                     ACNLKE"
BASE COUNT         5168 a         5038 c         5283 g         5229 t
ORIGIN      
        1 cgggaaatta catatcaatg ctcgaattat ccatgacgtt accgattaaa gtgcaaaatg
       61 gtggattatt tatttcccgt ggcgtaggga ggcatcctgc aaggcggctg gagtcatggg
      121 aaattatctt tgtcgaaaaa ggtcgattaa cgattcagga agaagaacgg atatttctgg
      181 tcgatgcggg agagagttta ttactctggc cgaaccgaca acatgttggg gtggaggatt
      241 ttcccgccgc tctcaaattt tactggctgc attttgaggt taaagcgccg gacaacgacc
      301 ctcgctggct tactcatctt agcgtgccgc aacaaacgaa ggttgtcgat ccgcaggcgc
      361 tggtggccct gtttcgccag ttcttaagcg aacaggaaaa gcatcaacgc actccggcgc
      421 tggagtttat tgtgctgctc attttgcagc agctcacccg tgacgcgcgt cttgatgaac
      481 aggccgatgg cgcaggcacc acccttgcct ggaaagcgca gaaactgatc cgcacgcaat
      541 atcatcgccc gctttctacg gcgcttctgg cccgcgagct gtactgcaat gccgattatc
      601 ttgggcgcgt ctaccggcgg gtgtttcatc tgacgttaac agaagcaatc catcgccagc
      661 gggtgcgcga agccgaaaag ctgttaatta gcgaagcgcg atcgctcacg gaagtggcgg
      721 cgttgtgcgg gtttaatgac gttggctatt tccggcagat ttttcgcaaa cacaccggtt
      781 taacgccagc agcgtggaag cggcgttaca gcaaggaaca tatcaattcg tagtgccgtg
      841 tcgatttcgc tttcgacatc acgtctgaaa caggtggggg agaaaaatct cctgaaacct
      901 ctgcgtgaaa ccgcgcaggc tatttctaat gaactggggt ttactgtcag ggacgaccag
      961 ggcgcgatca cataacgctt tggacaagtg ccaaaacttt aacatttcct tcgttggatc
     1021 aaagcagtag gggcgcgttc tctggcactc tgctgtttta gtgcaaaggg gtgatcatga
     1081 accggtttat tattgcggat gcgacgaaat gtatcggttg ccgtacctgt gaagtggctt
     1141 gcgcagtgtc gcatcaggaa aatcaggatt gcgctgcgtt gtcaccagac gagtttattt
     1201 cccgtattcg tgttattaaa gaccacagct ggaccacggc agtagcctgt catcagtgtg
     1261 aagatgcacc gtgcgcgaat gtctgccctg ttgacgcgat aagccgcgaa catgggcata
     1321 ttttcgttga acaatcacgt tgcattggct gtaaaagctg tatgctggct tgcccgtttg
     1381 gtgcgatgga ggtcgtttct tcgcgcaaaa aggcgagggc gattaagtgc gatctgtgct
     1441 ggcatcgggc gacgggaccg gcctgtgttg aagcctgccc gacaaaggcg ttgcagtgca
     1501 tggatgtcga gaaagtgcag cggcatcggc tacggcagca gcctgtttga aacgttttcc
     1561 ggcgtttctc aacgccggaa tagtgcgctt tgtttatgcc agatgcggcg taaacgcctt
     1621 atccggccta caaattcttc caaattcaat atattgcaga aatcatgtag gcctgataag
     1681 cgtagcgcat caggcaattt ttagtgactt tcagcccagg ctctttctat ctcttccgcc
     1741 agaatcttca cccccgcctc aattttctcc ggctctggta cgtagttcat gcgcatacat
     1801 tgatgcgtat gcggccacgg tttatccagc cctgggaaga agttgtgccc cggcaccatc
     1861 agcacgccgc gtgctttcag gcgctggtag agctgctcgg tcgtaatggg caaatcctta
     1921 aaccatagcc agaggaaaat ggctccttct ggtttatgaa tcaggcagcg atcttccggt
     1981 aaatagcggc gaatgatggc gatagtttcc tgaacacgct ggtagtaaaa cggtttgatg
     2041 actgtttcag acaggcgcag cagatcgtta cgcttaatca tttcacacat catcgccgga
     2101 ccaataccgc caggtgccag gctgataatg ccgttcatat tggtgatggc ggtgatgatt
     2161 ttttcattgg cgatgataat gccgcagcgg gagccaggta gacccagctt tgaaagactc
     2221 atgcacagca cgatattcgg attccatagc gggcgcgctt cactgaagat gatacccggg
     2281 aacgggacgc cataagcgtt atcaatcacc agcggaatgc cgtgttgatt cgccagcgcg
     2341 tcaagcttca gcaactcttc gtcagtaatc acattgcctg ttggattcgt cggccgggag
     2401 acgcaaatca tcccggtttc ttcgccaata tgcagatgct caaaatcgac gtggtattta
     2461 aactggcctt caggcagcag ttcaatattc ggacgcgcag agacaaacag atcttcttcc
     2521 agtccggcgt cagcgtagcc aatgtattcc ggtgccagcg ggaacagcac ttttttgacc
     2581 cgaccatcgg cacggcgtcc ggcaaacagg ttaaataagt agaaaaacgc gctctggctg
     2641 ccgtttgtta gtgcaatatt ctgtggttcg atatcccaac ccaacttctc gcgcagcatt
     2701 ccggcaagca gtgtgagtag ctccgttttc ccctgtggac cgtcgtaatt gcacagtgca
     2761 tcagtcgctt tgccactttc cagcatgtcg gtcagtagcg tctggaagta gtcctgcatt
     2821 tccgggatct gcgccggatt accgccgccg agcataatcg cgccaggcgt gcgtaaaccg
     2881 tcgttcagat cttccatcag cagcgtaatg ccggagtggc gggtaaattt gtcaccaaaa
     2941 agggagaatg tcatagcggg atatctgtcg aactctgtag caaggaaggt aacaataacg
     3001 ctacatttgc tggggtgcaa atcggtctgt tgaagggtga gcggtacttt atcctgcaac
     3061 gttaattagg gatgacattt tatcctggtt ggcggtttcc gaggtactga cgaacgggaa
     3121 gccggaaaag ttactgttgc cctgcccaga cgaccagcac tttatcgtcg ccatgctcac
     3181 gaacaaagcc gtagccctgc ttcagcgaaa gtgtcgtttg tttgcccgcg ccaattgcgg
     3241 gatgacgggc gcggaactgg ctgattttct gccagtgcgc gacgctggcg gcagatttac
     3301 cgctaacatc ctgccagttc atatccgaac gcgtaccttg cagcggatca gaaccggtag
     3361 gaccgaacgg acgcgaggat tcatcaccat aaaatatttg taccgcgcct ggcgctaata
     3421 ataataactc tgctgctttg tcgcccccgt cacggaacag gcgggtatca tgcgatgaga
     3481 ggtagctcaa cacattgaaa tcatgcaatt tctctgccat ttgctgccag gtcgtatcca
     3541 tctgcgccag acaatcgact gctttcgccg cctgctcctg ataatcgaaa ttgatcatcg
     3601 catcgaagcc gtggcgatag tagtcacttt gcatcacgcc gtggccccag gcttcaccgg
     3661 tcatccagaa aggtttgtca tctaatgctt tgtcggggtt agcttttttc cattcgcgaa
     3721 gcgcggcgct ggcttcggtt ttcagttgct gccaggcggg caactcaaca tgtttggcgg
     3781 tatcgactcg aaaaccatca atcccatagt cgcggaccca ctgacttaac cagtgggtta
     3841 agtaatcgcg aggggtaaag ccgtcgatga ctttagcgtg ggtatccgtt ttgtttttat
     3901 agaacaccgg cagaccagaa gcggtcgttg attcggtttt gatatccggc aaaaaggcca
     3961 gcgacatagt gagatcgtca aatccaggat tgtcgtaatc accgatatcc gttctgatcc
     4021 agttttttcc ccaccattta tcccagcctg ttttgtcgct gaaattaatg taatcgttaa
     4081 agctatgcca ggtttgcccg gcggcaggtt tccagtcact ccagcgttca cccagcgttt
     4141 ttgtcagctc gtcaccagaa agatataacg cgccaaactg aaactcctgc atatccgtca
     4201 gcgtggcata gccagtgtgg ttcatcacga catcaaagag aatacgaata ccacgctgat
     4261 gtgcgttatc aaccagcgtc cgtagatcgg cttcgctgcc catattggca tcaagattcg
     4321 tccagtcttg tgtgtaataa ccgtggtagg cataatgcgg gaaatcacct tttgtaccgc
     4381 ctccgaccca gccgtgaatt tgctcaaatg gggcgcttat ccacaaagca ttgacgccca
     4441 gctgctggag ataatccagt ttgttggtca ggccgcgtaa atcgccgccg tgaaaagtac
     4501 ctatttctgc cataccgtct ttatgacgtc cgtaactctg gtcattactg ggatcgccgt
     4561 tttcgaaacg atctgtcagc acaaagtaaa ccgtggcgtt atgccagtcg aaaggggcag
     4621 gggcgtcagt ttctgcccgt tccagcagga gtaaaccgtt gctggtggca gcgggttgta
     4681 acgttatttg accgttcttc actatcgcaa tttgctggct gtaataatcc cgtacggcgg
     4741 ctccttccgg aaaagtggcg ctgacatcca gcgtgagcgg taatccatcc catttcgggc
     4801 atacacggac ctggcttgct accggttcgg cggcgttctg gatggaaatc atcaatgttg
     4861 gcgtgccgga gcgggtgtct atttgcagcg tatattcgcc gtccctgaac aatcgccatt
     4921 gaggcggcgt gttgctacaa ggttgcaggg aaagcatctg attgagtttt atcacatccg
     4981 caggctgcca gcactgttgg tcaaaattta gcgttagtgg acgcgtacct ttgggcaact
     5041 gcgcgtggct gacaaatgtt cctgttccct gttcgctaaa ggcgggaaac cccggagaag
     5101 tccagctggt ggcaacggcg aagccaggaa ggagtgtcag aaaacaggcg gcgagtttca
     5161 tggcggatga gtccttattg ctgcgatttc agacagtttg ccagcgataa gccaaacaaa
     5221 actcatccct gagcggtaag tccacaggat gagaagcaag ggtgagcgat ctcgcgcaaa
     5281 aaacggctga attttgcgat aaaccccaca ttttctgcga tttagcgcca atctgaatcg
     5341 ttaacacgtg acatagtttc agatttggac tattccttgg ggtgctcttg acagctattt
     5401 ttgatatgat ttgagattca actctcaaat ttgtgaaaaa tataaggtgt tggaatgatt
     5461 aaatccgacc aggagacctg atgattttga ctcccatacg acgatatggg gcgatgattc
     5521 ttatgttact cactctggtg ttttcgagtg aggtgttagc gaagacgcac acaacaacag
     5581 cgagtcaaaa gtcccactta actaaagcta gtaataaaca ggtaagcagt aaacaagagt
     5641 attctcgcaa tagtgcaaag agtagttcac ttcctgattt gcgaaaatac ccttccggaa
     5701 caccaaggaa aaaggcgttt ctccggaccg taatgcctta cattaccagc caaaatgcag
     5761 ccattactgc agaacgtaac tggctgattt caaaacagta tcagggccaa tggtcacctg
     5821 ctgagcgtgc gcgtctgaaa gacatcgcca aacgctacaa ggtgaagtgg tctggtaata
     5881 cgcgaaaaat cccgtggaat accttgcttg aacgcgtaga cattatccct accagtatgg
     5941 tggcgacgat ggctgcagca gaaagcggtt ggggaacgtc gaagctggcg cgcaacaaca
     6001 acaatctttt cggcatgaaa tgcatgaaag gacgttgtac caatgcgccg ggtaaagtga
     6061 aagggtactc acagtttagt tctgtcaaag aatcggtgag cgcctatgtc actaacctca
     6121 atacgcatcc ggcttactct tcgttccgta aatcgcgtgc gcagctgcgt aaagctgatc
     6181 aggaagtgac tgccacagcg atgattcaca agctgaaggg ctactcgacc aaagggaaga
     6241 gttataacaa ctacctgttc gcaatgtacc aggataacca acggttaatt gcggcgcata
     6301 tgtaattgta tttcctcgag ccttatccga cttgtcggtc ggataaggct tttgatcgca
     6361 tcaggtggtt gtgctgagtt acctgatgcg acgccgacaa ttctcatcat cgctacaaca
     6421 tgacctcgct atttacatcg cgatactctt ttggcgtcgt gtcatatgct tttttaaaaa
     6481 cagagtagaa atattgcagc gatggataac cgcacatttg cgatatctca ttgatcgaca
     6541 aggtggttga aatcagcaga ctgcgcgctt tctccagctt ctcggcatga atcatggcat
     6601 ggatggtttc acccacctct tctttaaaac gcttctcaag attggagcgc gagatcccga
     6661 ccgcatcgag tacctgatcc actttaatcc ctttacaggc gtgattacga atgtaatgca
     6721 tggcctgaat aacggcggga tcggtcagcg aacggtaatc tgttgagcgc cgttcaatga
     6781 cgcgaactgg agggaccaaa atccgctgta gcggcatttc ttctttatct aataatcgat
     6841 gcaacagttt tgccgcctga tagcccattt gccgcgcgcc ctgagcgacc gaagaaaggg
     6901 cgacacgcga cagatagcgg gtcagttctt cgttgtcgat gccaatcacg cataattttt
     6961 ccggtacggg aatgtgtaga tgttcacata cttgcagaat atgccgcgct cgggcgtcag
     7021 taacggcaat aatcccggtt tgcggtggca gcgtttgtag ccagtctgcc agccgatttt
     7081 gcgcgtgttg ccagttctct ggcgcggttt ctaacccctg ataaaccact ccgcgatact
     7141 tttcttcggc gacaagctga cgaaatgcat attcgcgttc agtggcccaa tgtttgccgc
     7201 ttgattccgg aagaccataa aaagcaaagc ggttaacgcc tttctctttt aaatgcaaaa
     7261 atgcgctttc aaccagcgca tagttatcgg tggcaatgta atgaacgggt gggtaacttt
     7321 ctgcaaggtg atacgagccg ccaaccccaa caatggggac gtcgacatca gccagcgctt
     7381 gctcgatttg tttgtcgtcg aagtcggcaa tgacgccatc tcctaaccag tccttgattt
     7441 tatcaatgcg ggcgcggaaa tcttcttcaa tgaaaatatc ccattccgat tgtgacgcct
     7501 gtaaatattc ccctacgccc tctactacct gccggtcata ggctttattg gcattgaaca
     7561 gtaatgtgat gcggtgacgt ttagtaaaca tggttctttt cctgctgaat catgcaaaaa
     7621 ctcaaaaccg gtaatacgtt accggctttg agaaaatttt tatcaaaatc aagaacggcg
     7681 tttggttgcg gagtccatcc atactgccag caacagaatc gcacctttaa cgatatactg
     7741 ccagaaggtc ggtacatcca tcatactcat accgttatcc agtgaagcca tgatgaatgc
     7801 ccccattacc gctccagcca cgctcccgac gccgcctgcc agactggtgc cgccaatcac
     7861 gcatgctgca attgcgtcca gctcggcgat attccccgca gaaggtgaac cagcgccaag
     7921 tcgagaactg aggattaatc cggcgatggc taccattaat ccgttaatag cgaacacggc
     7981 aagtttggtg cgttcaacgt taatcccgga gagacgtgct gcttccagat tgccgccgat
     8041 ggcataaatg cgtcgtccaa atgccgtccg cgttgccata aacattccgc cgagtaacag
     8101 caacgtcagc agcagaacag gagtgggaac gccacgataa tcattcaaca gccagattgc
     8161 gcctaatacg atgatagcgg ttaaagcctg gcgaccgact actgcggtag aggccggaga
     8221 ctgcaaaccc aaagcctgac ggcgcattct tccgcgccat tgccagccaa caaaagccat
     8281 taagccaagc gcgccaatga tgaagccggt gctggcgggg agatagcttt gcccaatttg
     8341 tgacatcgcg gcgctggtgg gggatacagt cgtgccgttg gtgatgccaa tgagtatgcc
     8401 gcgaaatgcc aacatgcccg cgagagtgac aataaatgaa gggactttgc ggtacgcgac
     8461 ccaccatccg ttccaggcac cgagaagcag tcccagaacc aacgtcacaa tgatggtaag
     8521 tggcaaaggc cagcctagcc agacgtcaca aatcgccgcg acgccaccta atagccccat
     8581 cattgagccg acggaaaggt cgatttcagc agaaattatg acgaacacca ttcctaccgc
     8641 gaggatgccg gtaatcgcgg tctggcgtaa caggttggag acgttacggg cgcttaagta
     8701 ggcaccatcg gtggtccagg taaagaacag catgattgcg atgatagctg caatcatcac
     8761 gaagacctgc aaattcagtg atttcagccc ggagaagcca ccggatgtcg gtacggccaa
     8821 tttcacttca gacggattgc ttttcgacat gatgttcgct cctcaatgcg gcttccatca
     8881 cctgctcctg agtcaggtta tgatttatca ggttggcttt tagtttccct tcatgcatca
     8941 ccagtacacg atcgctaagg ccgagcactt caggtaattc agaagagatg acaataacgg
     9001 caataccctg ctggacgagt tggttaatta atttgtagat ctcgtatttc gcgccaatat
     9061 cgatacccct ggtgggttca tcaagaatga gaatgcgcgg gttaagtaac agacagcgag
     9121 cgaggatcgc tttttgctga ttgccgccgc tcaaacgtcc aatagcaagg tcgggggacg
     9181 acgttttaac tttgagttgc tggattgatt ccagaataca tttttgctct gccgcgtcat
     9241 caagctggct aatgccaccg gtaaatttat tgagtgcggc gagggtaata tttttaccaa
     9301 ccgccattac cggaacgatg ccgtcgcgct ttctgtcttc ggggaccatc gcaatcccct
     9361 gggcgatggc ttgctgacag ttacgaatat ctacctgttt gccatcaata taaatttttc
     9421 cttcccattg tccgggccac acaccaaaca ggcactgaat ggtctcggta cgtccggcac
     9481 caacgagtcc ggcaataccc agtatttcgc cacgtttcag ggaaaacgag acatcattaa
     9541 ctcgtttaat atgacgatta accggatgcc atgccgtcag atgttcaata cgtaatattt
     9601 catctccggt ggtatgtggt tcattagggt aaagcgcggt taactctcgc ccgaccatca
     9661 tggtgataat atcgtcttca ctcattccgg cagcatcacg cgtaccaatg tgctgtccgt
     9721 cgcgaataac gcaaatcgta tcggaaatcg ctttgacttc gttgagtttg tgcgaaatat
     9781 aaatacaggc gataccgtgc tgttgtagat cgcgaataat atccagtaaa accgacgttt
     9841 cctgctcagt taatgaggct gtcggttcat cgagaattaa caagcgcacc tgtttattaa
     9901 gtgccttggc aatttcaacc agttgttgtt gcccaagccc taaatcgcca acgcgggtat
     9961 caggtgaaat ggataaactg acctgtgcga gcagcttctg acagcgtagc gtcatcaggt
    10021 cataatccat aatgccattg tgggttattt cgttacccag gaagatattt tccagcacgg
    10081 tcaattcttt caccagggcc aattcctggt gaatgatggc gatacctttg cgttcggtat
    10141 cgcggatgtg actcgcctga atctcttctc ccgcaaaaat aatttcgcct tcgtaggagc
    10201 catggggata aataccacac agcactttca tcagcgttga tttaccagac ccattttccc
    10261 cacagagtga gacgatttcg ccagcattca accgcaagct gacgttatca atcgccttca
    10321 cactgccgaa ggttttggta atgttcttca tttcaagtag ataaggcata acgactccac
    10381 ctaagccaat tcattcacgc ggcatggaga gaaatcacgc ccccgctccg cgctggggcg
    10441 taacgcttac agctcgctct ctttgtggaa tccgtcttta attaccgtat ctttgatgtt
    10501 gtttttattc acatcgatcg gtgtcaggag gcgggagggg acatctttca ggccattatt
    10561 cagtgaggta tctgcttttg gctcctgacc attgcccaat tcaacggcaa tttctgcggc
    10621 agtatttgcc agcaatgtaa taggtttata taccgtcata gtttgcgtac cggcagcaat
    10681 acgtttaata cctgcgagat ccgcatcctg gccggaaatt gctactttcc ctgataaacc
    10741 ttgcgcgctt aatgcctgaa ttgccccacc tgcggtggca tcgtttgagg caactacagc
    10801 atcaattttg ttattattgg cggttagcgc gttttccata attttcaatg cgttttccgg
    10861 taaccagcca tcaacccatt ggtcaccaac gactttaatt tttccggaat caacgtaagg
    10921 ttttaacact ttcatttgtc cggcgcggaa cagcttggcg ttgttatcta ccggcgagcc
    10981 gcccatcagg aagtaattac cttgcggaac aatatcgacc agggcttttg cctgcagttc
    11041 accgactttt tcgttatcga aagaaatata aaaatcgata tccgcatcgt taatcatacg
    11101 gtcgtaagct aatactttaa tgccttcttg tttggcttct tttacaacgt tacttaatac
    11161 ctgaccgtta tacggaataa tgacaagaac atcgacaccc cggtttatca tgttttcaat
    11221 ctgcgacatt tgtgtttctt cattgccatt tgcagactgt acaaatactt tcgcgccgag
    11281 agattctgcc tttttcacaa aaatatctcg atctttttgc cagcgttcaa gacggagatc
    11341 atcaatcgcc atacctattt tgacttcttt ggcgtgtgcc gcaacgttgg taagcagaag
    11401 tgaggtgcaa agggtgagta gaatgttctt tattttcatg gtgtagggcc ttctgtagtt
    11461 agaggacagc tttaataagt aacaatcacc gcgataaacg taaccaattt ttagcaacta
    11521 aacaggggaa aacaattaca gatttttatc tttcgattac gatttttggt ttatttcttg
    11581 atttatgacc gagatcttac ttttgttgcg caattgtact tattgcattt tcctcttcga
    11641 ggaattacca agtttcatca ttccatttta ttttgcgagc gagcgcacac ttgtgaatta
    11701 tctcaatagc agtgtgaaat aacataattg agcaactgaa agggagtgcc caatattacg
    11761 acatcatcca tcacccgcgg cattacctga ttatggagtt caatatgcaa gcctattttg
    11821 accagctcga tcgcgttcgt tatgaaggct caaaatcctc aaacccgtta gcattccgtc
    11881 actacaatcc cgacgaactg gtgttgggca agcgtatgga agagcacttg cgatttgccg
    11941 cctgctactg gcacaccttc tgctggaacg gggcggacat gtttggtgtg ggggcgttta
    12001 atcgtccgtg gcagcagcct ggtgaggcac tggcgttggc gaagcgtaaa gcagatgtcg
    12061 catttgagtt tttccacaag ttacatgtgc cattttattg cttccacgat gtggatgttt
    12121 cccctgaggg cgcgtcgtta aaagagtaca tcaataattt tgcgcaaatg gttgatgtcc
    12181 tggcaggcaa gcaagaagag agcggcgtga agctgctgtg gggaaccgcc aactgcttta
    12241 caaaccctcg ctacggcgcg ggtgcggcga cgaacccaga tccagaagtc ttcagctggg
    12301 cggcaacgca agttgttaca gcgatggaag caacccataa attgggcggt gaaaactatg
    12361 tcctgtgggg cggtcgtgaa ggttacgaaa cgctgttaaa taccgacttg cgtcaggagc
    12421 gtgaacaact gggccgcttt atgcagatgg tggttgagca taaacataaa atcggtttcc
    12481 agggcacgtt gcttatcgaa ccgaaaccgc aagaaccgac taaacatcaa tatgattacg
    12541 atgctgcgac agtctatggc ttcctgaaac agtttggtct ggaaaaagag attaaactga
    12601 acattgaagc taaccacgcg acgctggcag gtcactcttt ccatcatgaa attgccaccg
    12661 ccattgcgct tggcctgttt ggttctgtcg acgccaaccg tggcgatgcg caactgggct
    12721 gggacaccga ccagttcccg aacagtgtgg aagagaatgc gctggtgatg tatgaaattc
    12781 tcaaagcagg cggtttcacc accggtggtc tgaacttcga tgccaaagta cgtcgtcaaa
    12841 gtactgataa atatgatctg ttttacggtc atatcggcgc gatggatacg atggcactgg
    12901 cgctgaaaat tgcagcgcgc atgattgaag atggcgagct ggataaacgc atcgcgcagc
    12961 gttattccgg ctggaatagc gaattgggcc agcaaatcct gaaaggccaa atgtcactgg
    13021 cagatttagc caaatatgct caggaacata atttgtctcc ggtgcatcag agtggtcgcc
    13081 aggagcaact ggaaaatctg gtaaatcatt atctgttcga caaataacgg ctaactgtgc
    13141 agtccgttgg cccggttatc ggtagcgata ccgggcattt ttttaaggaa cgatcgatat
    13201 gtatatcggg atagatcttg gcacctcggg cgtaaaagtt attttgctca acgagcaggg
    13261 tgaggtggtt gcttcgcaaa cggaaaagct gaccgtttcg cgcccgcatc cactctggtc
    13321 ggaacaagac ccggaacagt ggtggcaggc aactgatcgc gcaatgaaag ctctgggcga
    13381 tcagcattct ctgcaggacg ttaaagcatt gggtattgcc ggccagatgc acggagcaac
    13441 cttactggat gctcaacaac gggtattgcg ccctgccatt ttgtggaacg acgggcgctg
    13501 tgcgcaagag tgcgctttgc tggaagcgcg agttccgcta tcgcgggtga ttaccggcaa
    13561 cctgatgatg cccggattta ctgcgcctaa attgctatgg gttcagcggc atgagccgga
    13621 gatattccgt caaatcgaca aagtattatt accgaaagat tacttgcgtc tgcgtatgac
    13681 gggggagttt gccagcgata tgtctgacgc agctggcacc atgtggctgg aagttgcaaa
    13741 gcgtgactgg agtgacgtca tgctgcaggc ttgccactta tctcgtgacc agatgccagc
    13801 attatacgaa ggcagcgaaa ttactggtgc tttgttacct gaagttgcga aagcgtgggg
    13861 tatggcggcg gtgccagttg tcgcaggcgg tggcgacaat gcagctggtg cggttggtgt
    13921 gggaatggtt gatgctaatc aggcaatgtt atcgctgggg acgtcggggg tctattttgc
    13981 tgtcagcgaa gggttcttaa gcaagccaga aagcgccgta cacagctttt gccatgcgct
    14041 accgcagcgt tggcatttaa tgtctgtaat gctgagtgca gcgtcgtgtc tggattgggc
    14101 cgcgaaatta accggcctga gcaatgtccc agctttaatc gctgcagctc aacaggctga
    14161 tgaaagtgcc gagccagttt ggtttctgcc ttatctttcc ggcgagcgta caccacacaa
    14221 taatccccag gcgaaagggg ttttctttgg tttgactcat caacatggcc ccaatgaact
    14281 ggcgcgagca gtgctggaag gcgtgggtta tgcgctggca gatggcatgg atgtcgtgca
    14341 tgcctgcggc attaaaccgc aaagtgttac gttgattggt ggcggggcgc gtagtgagta
    14401 ctggcgtcag atgctggcgg atatcagcgg tcagcagctc gattaccgta cgggggggga
    14461 tgtggggcca gcactgggcg cagcaaggct ggcgcagatt gcggcgaatc cagagaaatc
    14521 gctcattgag ttgttgccgc aactaccgtt agaacagtcg catctaccag atgcgcagcg
    14581 ttatgtcgct tatcagccac gacgtgaaac attccgtcgc ctctatcagc aacttctgcc
    14641 attaatggcg taaacgttat cccctgcctg accgggtggg ggataattca catctatata
    14701 tctcagcaat taattaatat ttagtatgaa tttattctga aaatcatttg ttaatggcat
    14761 ttttcagttt tgtctttcgt tggttactcg taatgtatcg ctggtagata tggagatcgt
    14821 tatgaaaacc tcaaaaactg tggcaaaact attatttgtt gtcggggcgc tggtttatct
    14881 ggttgggcta tggatctcat gcccattgtt gagtggaaaa ggctattttc ttggcgtgtt
    14941 aatgacagca acttttggca actatgcata tcttcgcgca gaaaaactca ggcaactgga
    15001 taattttttt acccatattt gccagttagt tgcgttaatc actatcggtc tcttgtttat
    15061 cggtgtttta aatgcaccta tcaatgctta tgaaatggtg atctacccca tcgccttttt
    15121 tgtctgcttg tttggtcaaa tgcgtttgtt tcgctcggta tgagcaacat aaagctctta
    15181 tacattcagg aatgaaagga atactgtgat ggacaacaaa atatcaacct attcaccggc
    15241 ctttagtatt gtgtcatgga tagctctcgt tggtggtatc gttacctatc tgttagggct
    15301 atggaatgca gagatgcagt taaatgaaaa aggatattat tttgccgtac tggtattagg
    15361 actgttttct gcggcgtctt atcaaaagac cgttcgggac aaatatgaag gcataccgac
    15421 cacttccatt tattatatga cctgcctgac tgtctttatt atctctgttg cattactgat
    15481 ggtaggtctg tggaatgcga cattattact cagcgaaaaa ggtttttatg gactggcttt
    15541 cttcttaagc ttgtttggtg cagtagcggt gcagaaaaat attcgtgatg ccggaataaa
    15601 cccaccaaaa gaaacacagg ttacccagga agaatacagc gaataactca cgtaagcccg
    15661 gtcagtccaa tgtgaccggg cttttactta actcactaat ctgtttctgt cgattcgttg
    15721 taccagcata gaaagtaaca aactcgctgc caacgtcgcg caaaaaatcc aaataatatc
    15781 cagtattggc caatttttaa gctcaattcc ccgggtgcgc agcgcatgga taatcaaggc
    15841 gtggaatccg tatataccca acgaatggcg ggagattaag ccaagtccgc gaatggtacg
    15901 cgtatccagc gtgtttttaa ccagagtcaa tagcgcgatt gcgcagataa aaaccatcgg
    15961 cccacagtaa agataccagg tatcggcaaa atttccgcgc cactgcaatt catataatgt
    16021 cccgcgagag ataataaaaa ctcccgtcgc aaacagtgcg gcgctcaccc acgacagtgc
    16081 tttatgctgt gtgtccatca tccctaaagc gcggcccaac atgccataca gaatgtagta
    16141 aaaagtatcg ccattgatat ataagttaat tggcagccat tcaaaaccgt cgattttctg
    16201 cggcacggtg tttgggttag cgatgatacc aatcaccacc attagcacca gcaacatttt
    16261 tccgccgacg ttcttcacct gaatcagcgg tgaaaccaga taaatcaccg caatcgcgaa
    16321 gaaaaaccat aagtggtaaa acactggctt ttgcagcagg tttttcagcg ctaactccac
    16381 attaatggag gtaaacagtg caatgtagag cagcgcgatt gcgctataaa aaaacagaca
    16441 taagccgata cgcaagaaat ggcgcggctg ggcgctgcgt tcgccaaaaa agagatagcc
    16501 ggaaatcatg aaaaatagcg gcacgctgac acgagaggca gagttcagaa cattggcgat
    16561 atcccaggtg acggggctaa cactatgagc attggtcaca taccaggtag tggtgtgaat
    16621 catcaccacc attaaacacg ctatccctcg caggttatca atccagtaaa ttttgggctg
    16681 catctgtgtc tctgtatctg gttaaaaaaa gtctgaccga taaatcattc agttggcgca
    16741 ctggaataat ctgagtttta acactacagc ttatagaggc ttaaggaaat tcgtaagata
    16801 tcagccacta taccgatata aataataaga ctcacctgca aaccagacgg taatttaatg
    16861 atgatgaact ctttctttcc ggcaatggcg cttatggtgc tagtgggttg ttctacaccg
    16921 tcacccgtgc agaaagcaca acgggtaaag gttgatcctc tgcgttcgtt gaatatggaa
    16981 gcgttatgca aggatcaggc ggcaaaacgt tataacaccg gcgagcaaaa aatcgacgtc
    17041 accgccttcg aacagttcca gggaagctat gaaatgcgcg gttatacctt ccgtaaagag
    17101 cagtttgtct gttcttttga cgcagatggc cattttttgc atctttccat gcgttaagcc
    17161 ctgctttttc ccgtttcata ctgtatatct tccatccagc gggtatactg atcccttcct
    17221 ttaaatccac acgtatccag cacgaaataa tatgcaaaag tttgatacca ggaccttcca
    17281 gggcttgatc ctgaccttac aggattactg ggctcgccag ggctgcacca ttgttcaacc
    17341 attggacatg gaagtcggcg cgggaacctc tcacccaatg acctgtctgc gcgcgctggg
    17401 gccagaaccg atggcggctg cttatgttca gccttctcgt cgcccgaccg atggtcgcta
    17461 cggcgaaaac cccaaccgtt tacagcacta ctatcagttc caggtggtca ttaagccatc
    17521 gccggacaat attcaggagc tgtaccttgg ttctctgaaa gagctgggca tggacccgac
    17581 tatccacgat atccgtttcg tggaagataa ctgggaaaac ccgacgctgg gtgcctgggg
    17641 actgggctgg gaagtgtggc taaacggcat ggaagtgacg cagttcactt acttccagca
    17701 ggttggtggt ctggagtgta aaccggttac cggcgagatc acctacggtc tggaacgtct
    17761 ggccatgtac attcagggcg tagacagcgt ttacgacctg gtctggagcg acggcccgct
    17821 gggtaaaacc acctacggcg acgtgttcca tcagaacgaa gtggagcagt ccacttacaa
    17881 cttcgaatac gcggacgtgg acttcctgtt cacctgcttc gagcagtacg agaaagaagc
    17941 gcagcagctg ctggcgctgg aaaatccgct gccgctgcca gcttacgagc gtattctgaa
    18001 agccgcccac agcttcaacc tgctggatgc gcgtaaagcc atctccgtca ccgagcgtca
    18061 gcgctacatt ctgcgcattc gcaccctgac caaagcagtg gcagaagcat attacgcttc
    18121 ccgtgaagcc ctcggcttcc cgatgtgcaa caaagataag taagaggcgg ctatgtctga
    18181 gaaaactttt ctggtggaaa tcggcactga agagctgcca ccaaaagcac tgcgcagcct
    18241 ggcagagtcc tttgctgcga actttactgc ggagctggat aacgcaggcc tcgcacacgg
    18301 caccgttcaa tggtttgctg ctccgcgtcg tctggcgctg aaagtagcta acctggcgga
    18361 agcgcaaccg gatcgtgaaa tcgaaaaacg cggcccggcg attgcccagg cgttcgacgc
    18421 tgaaggcaaa ccgagcaaag cggcagaagg ttgggcgcgt ggttgcggta ttaccgttga
    18481 ccaggctgag cgtctgacta ccgataaagg cgaatggctg ctgtatcgcg cccatgtgaa
    18541 gggcgaaagc actgaagcac tgctgccgaa tatggttgcg acttcgctgg cgaaactgcc
    18601 gattccgaaa ctgatgcgct ggggtgcaag cgacgtgcac ttcgtgcgtc cggtgcacac
    18661 cgtgaccctg ctgctgggcg acaaagtcat tcctgcaacc attctgggca ttcagtccga
    18721 tcgcgtgatt cgcggccacc gctttatggg cgagccggaa ttcaccatcg ataacgccga
    18781 tcagtatccg gaaattctgc gtgagcgtgg gaaagtcatc gccgattacg aagaacgtaa
    18841 ggcgaagatt aaagccgatg ccgaagaagc agcgcgtaag attggcggta acgctgactt
    18901 aagcgaaagc ctgctggaag aagtggcttc gctggtggag tggccggtcg ttctaaccgc
    18961 gaaatttgaa gagaaattcc tcgcggtgcc gtctgaagca ctggtttaca ccatgaaagg
    19021 tgaccagaag tacttcccgg tgtatgcgaa cgacggcaaa ctgctgccga actttatctt
    19081 cgttgccaac atcgaatcga aagatccgca gcaaattatc tctggtaacg agaaagtcgt
    19141 tcgtccacgt ctggctgatg ccgagttctt cttcaacacc gaccgtaaaa aacgtctgga
    19201 agataacctg ccgcgtctgc aaaccgtgct gttccagcaa cagctgggta cgctgcgtga
    19261 caaaactgac cgcatccagg cgctggctgg ctggatcgcc gaacagattg gcgctgacgt
    19321 taaccacgca acccgtgcgg gcctgctgtc taagtgtgac ctgatgacca acatggtctt
    19381 cgagttcacc gacacccaag gcgttatggg gatgcactac gcgcgtcacg atggcgaagc
    19441 ggaagacgta gccgtcgcgc tgaatgagca gtatcagccg cgctttgccg gtgatgacct
    19501 gccgtccaac ccggtagctt gtgcgctggc gattgctgat aagatggata ctctggcggg
    19561 tatcttcggt atcggtcagc atccgaaagg cgacaaagac ccgtttgcgc tgcgtcgtgc
    19621 cgcacttggc gtgctgcgaa ttatcgttga gaagaacctc aaccttgatc tgcaaacgct
    19681 gaccgaagaa gcggtgcgtc tgtatggcga taagctgact aatgccaacg tagttgatga
    19741 tgttatcgac tttatgctcg gtcgcttccg cgcctggtat caggacgaag gttataccgt
    19801 tgacaccatc caggcggtac tggcgcgtcg tccgactcgt ccggctgatt tcgatgcccg
    19861 catgaaagcg gtatcgcact tccgtaccct ggaagcagct gcagcactgg cggcggcgaa
    19921 caaacgtgta tctaacatcc tggcgaaatc tgacgaagtg ctgagcgatc gcgtgaatgc
    19981 ctctactctg aaagagccgg aagaaattaa actggcgatg caggttgtgg tgctacgtga
    20041 caagctggag ccgtactttg cggaaggtcg ttaccaggat gcgctggtcg aactggcaga
    20101 gctgcgtgaa ccggtcgatg ccttcttcga taaagtgatg gtcatggttg atgacaaaga
    20161 attgcgtatc aaccgtctga ccatgctgga gaaactgcgc gaactgttcc tgcgcgttgc
    20221 ggatatttcg ctgttgcagt aataacgccg ttattaaaaa gcctgccatc tggcaggctt
    20281 tttttatcgc taaataatac agcaaccttt aataatcttc tgctgaataa agattatctc
    20341 atatattaat tttatgagat ttttttagga ttatatcaag gagaagaaac aaacttatta
    20401 agctagaata gccacgggtg cttgagactg tttgtctcag gtattcaccg aaaggcagac
    20461 agagaaaagc cccacctgac tataaatcaa agtgaggccc ctctatgcat agacaccata
    20521 aggttagcct cttactcgtt ggaaatcaac acagggggct gggaatgccg cagaaatata
    20581 gattgctttc tttaatagtg atttgtttca cgcttttatt tttcacctgg atgataagag
    20641 attcactgtg tgaattgcat attaaacagg ggggttatga gctggcggca tttttagcct
    20701 gcaatttgaa agagtaag
//
LOCUS       DAZUCE010000074        20374 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZUCE010000074 DAZUCE010000000
VERSION     DAZUCE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.074"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            26..1480
                     /locus_tag="ACHY03_002283"
     CDS             26..1480
                     /locus_tag="ACHY03_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFS3905504.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            1540..2901
                     /gene="allB"
                     /locus_tag="ACHY03_002284"
     CDS             1540..2901
                     /gene="allB"
                     /locus_tag="ACHY03_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFS3905505.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            2958..4259
                     /locus_tag="ACHY03_002285"
     CDS             2958..4259
                     /locus_tag="ACHY03_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFS3905506.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            4281..5426
                     /gene="glxK"
                     /locus_tag="ACHY03_002286"
     CDS             4281..5426
                     /gene="glxK"
                     /locus_tag="ACHY03_002286"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFS3905507.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(5554..6339)
                     /gene="allE"
                     /locus_tag="ACHY03_002287"
     CDS             complement(5554..6339)
                     /gene="allE"
                     /locus_tag="ACHY03_002287"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFS3905508.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(6350..7585)
                     /gene="allC"
                     /locus_tag="ACHY03_002288"
     CDS             complement(6350..7585)
                     /gene="allC"
                     /locus_tag="ACHY03_002288"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFS3905509.1"
                     /translation="MITHFRQAIEEALPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNEDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(7607..8656)
                     /gene="allD"
                     /locus_tag="ACHY03_002289"
     CDS             complement(7607..8656)
                     /gene="allD"
                     /locus_tag="ACHY03_002289"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFS3905510.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVIINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFIQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            8973..10640
                     /locus_tag="ACHY03_002290"
     CDS             8973..10640
                     /locus_tag="ACHY03_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFS3905511.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVTRDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLIADLGAKPSVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            10650..11909
                     /locus_tag="ACHY03_002291"
     CDS             10650..11909
                     /locus_tag="ACHY03_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFS3905512.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            11920..12735
                     /locus_tag="ACHY03_002292"
     CDS             11920..12735
                     /locus_tag="ACHY03_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFS3905513.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLVLAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            12732..13625
                     /locus_tag="ACHY03_002293"
     CDS             12732..13625
                     /locus_tag="ACHY03_002293"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS3905514.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(13824..14891)
                     /gene="purK"
                     /locus_tag="ACHY03_002294"
     CDS             complement(13824..14891)
                     /gene="purK"
                     /locus_tag="ACHY03_002294"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFS3905515.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPEECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPIV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(14888..15397)
                     /gene="purE"
                     /locus_tag="ACHY03_002295"
     CDS             complement(14888..15397)
                     /gene="purE"
                     /locus_tag="ACHY03_002295"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFS3905516.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(15515..16237)
                     /gene="lpxH"
                     /locus_tag="ACHY03_002296"
     CDS             complement(15515..16237)
                     /gene="lpxH"
                     /locus_tag="ACHY03_002296"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFS3905517.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARNADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(16240..16734)
                     /gene="ppiB"
                     /locus_tag="ACHY03_002297"
     CDS             complement(16240..16734)
                     /gene="ppiB"
                     /locus_tag="ACHY03_002297"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFS3905518.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            16908..18293
                     /gene="cysS"
                     /locus_tag="ACHY03_002298"
     CDS             16908..18293
                     /gene="cysS"
                     /locus_tag="ACHY03_002298"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFS3905519.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(18329..18850)
                     /locus_tag="ACHY03_002299"
     CDS             complement(18329..18850)
                     /locus_tag="ACHY03_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS3905520.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(18958..19170)
                     /gene="ybcJ"
                     /locus_tag="ACHY03_002300"
     CDS             complement(18958..19170)
                     /gene="ybcJ"
                     /locus_tag="ACHY03_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFS3905521.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(19172..20038)
                     /gene="folD"
                     /locus_tag="ACHY03_002301"
     CDS             complement(19172..20038)
                     /gene="folD"
                     /locus_tag="ACHY03_002301"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFS3905522.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
BASE COUNT         4646 a         5173 c         5598 g         4957 t
ORIGIN      
        1 cgatacgtga aattaagagg gatttatgga acatcagaga aaactattcc agcaacgcgg
       61 ctatagcgaa gatctattgc cgaaaacgca aagccagcgg acctggaaaa catttaacta
      121 ttttacctta tggatgggtt cggttcataa cgttcccaat tatgtgatgg tcggcggctt
      181 ttttattctc ggcttgtcta cctttagtat tatgctggca attatcctca gcgccttttt
      241 cattgccgcg gtaatggtat taaacggtgc tgcgggcagt aaatacggcg taccgtttgc
      301 catgatcctg cgtgcttctt acggcgtacg tggcgcactg tttcccggat tattaagagg
      361 cggtattgct gccattatgt ggtttggcct gcaatgttac gcagggtcac tggcctgctt
      421 gattctgatt ggcaaaatct ggccgggatt tttgactctc ggtggtgatt tcactctgtt
      481 aggcctttct ctaccgggct taattacttt cttactcttc tggctggtca acgttggtat
      541 aggttttggc ggtggcaaag ttttaaataa attcactgcc attcttaacc cgtgcatcta
      601 tatcgttttc ggcggtatgg cgatttgggc gatttcgctg gtcgggctcg gtccaatctt
      661 tgactacatt ccgagcggta ttcagaaagc agaaaacagt ggcttcctgt tcctggtggt
      721 gattaacgcg gtagttgcgg tctgggcggc tccggcggtg agcgcatccg actttacgca
      781 aaacgcccac tcgtttcgtg agcaagcgct ggggcaaacg ctgggtttag ttgtggccta
      841 tattctgttt gcggtcgccg gggtatgtat tattgccgga gccagtattc attacggcgc
      901 tgatacctgg aacgtgctgg atattgttca gcgttgggac agcctgtttg cctcgttctt
      961 tgcggtactg gttattctga tgacgaccat ttccactaac gccaccggta atattattcc
     1021 ggccggttat cagattgctg ccattgcacc gacaaaactg acctataaaa acggcgtact
     1081 gattgccagt attatcagcc tgctgatctg cccgtggaaa ttaatggaaa atcaggacag
     1141 tatttatctg ttcctcgata tcatcggcgg aatgctgggt ccggtaattg gtgtcatgat
     1201 ggcgcattat tttgtggtga tgcgtgggca aattaatctt gatgaactgt acaccgcacc
     1261 tggcgattat aaatattacg ataacggttt taacctcacc gcgttttcag taactctggt
     1321 ggccgttatt ttatctcttg gcggtaagtt tattcccttt atggaaccgt tatcgcgtgt
     1381 ttcatggttt gtcggcgtca tcgtcgcctt tgcggcctac gccttattaa agaaacgtac
     1441 aacagcagaa aaaacaggag agcaaaaaac cataggttaa ttaatcccga tattgaacat
     1501 tgagttaaaa accaatctgt attttacaag gagtttgtta tgtcttttga tttaatcatt
     1561 aaaaacggca ccgttatttt agaaaacgaa gctcgcgttg tagatatcgc cgttaaaggc
     1621 ggcaaaattg ctgctatcgg tcaggatctg ggcgatgcaa aagaagttat ggatgcgtct
     1681 ggtctggtgg tttcgccggg catggttgat gcgcacaccc atatttctga accgggtcgt
     1741 agccactggg aaggttatga aaccggtact cgcgcagcgg caaaaggtgg tatcaccacc
     1801 atgatcgaaa tgccgctcaa ccagctgcct gcaacggttg accgcgcttc aattgaactg
     1861 aagttcgatg ccgctaaagg caagctgact atcgatgcgg cacaactcgg tggcctggtg
     1921 tcttacaaca tcgaccgtct gcatgagctg gatgaagtgg gcgttgtcgg cttcaaatgc
     1981 ttcgttgcga cctgtggcga tcgcggtatc gacaacgact tccgtgatgt aaacgactgg
     2041 cagttcttca aaggtgcgca gaagctgggc gaactggggc agccggtgct ggtgcactgc
     2101 gaaaacgcgc tgatctgtga cgcactgggc gaagaagcga aacgtgaagg tcgcgtaact
     2161 gcccatgact atgtggcttc gcgtccggta tttaccgaag tggaagcgat tcgccgcgtg
     2221 ctgtatctgg cgaaagttgc cggttgccgt ctgcacgttt gccatatcag cagcccggaa
     2281 ggcgttgaag aagtgactcg tgcacgtcag gaaggtcagg acgttacttg tgaatcctgc
     2341 ccgcattact ttgtactgga taccgatcag ttcgaagaaa tcggtactct ggcgaagtgt
     2401 tcaccgccga tccgcgatct ggaaaaccag aaaggcatgt gggaaaaact gtttaacggt
     2461 gaaatcgact gcctggtttc cgaccactca ccatgccctc cggaaatgaa agccggcaac
     2521 atcatggaag catggggcgg tattgccggt ctgcaaaact gtatggacgt gatgttcgat
     2581 gaagcggtac agaaacgcgg aatgtctctg ccaatgttcg gcaaattaat ggcgactaac
     2641 gcagcagata ttttcggtct gcagcaaaaa ggccgtatcg ccccaggaaa agatgccgac
     2701 ttcgtcttca ttcagccgaa tagcagctat gttcttacca atgacgatct ggaatatcgc
     2761 cacaaagtca gcccgtatgt tggccgtacc attggcgcgc gtatcacgaa aaccatctta
     2821 cgtggtgatg tgatttacga catcgaacag ggcttccctg ttgcgccgaa aggtcaattt
     2881 atccttaaac atcagcagta atctggcccc tgcaatgccc gtccttgcgg cgggcattct
     2941 ccggttaagg tgtgtttatg ttcaattttg cagtcagccg cgaaagcctg ttatcaggat
     3001 ttcagtggtt tttctttatt ttttgcaaca cggttgtggt tcctcctacg ctactttctg
     3061 cttttcagtt gccgcaaagt agcctgctta cactcacaca atatgctttt cttgctaccg
     3121 cactggcctg cttcgctcag gcgttctgtg gccatcgtcg cgctattatg gaagggccag
     3181 gtggcctgtg gtggggaacc atcctaacta tcacccttgg cgaagcatcg cgcgggacac
     3241 cgatcaacga tatcgctacc agcctggcgg tggggattgc actctccggc gtgctgacga
     3301 tgttgattgg ttttagcgga ttaggtcatc gcctggcgcg gttatttacg ccgtcggtga
     3361 tggtcttgtt tatgttgatg ctgggcgcgc agctgaccac tatctttttc aaaggtatgc
     3421 tcgggctgcc gtttggcata gccgacccga attttaaaat tcagttaccg ccgttcgcgc
     3481 tctcggtggc ggtgatgtgc ctggtactgg cgatgattat tttcctgccg caacgttttg
     3541 cccgttatgg cctgctggtc ggcaccataa ccggctggtt gttgtggtac ttttgctttc
     3601 cttcttcgca ttcgctctcc ggtgagttgc actggcagtg gttcccgctc ggcagtggcg
     3661 gtgctttgtc gccggggatt attctgacgg cggtgattac aggtctggtg aatatcagca
     3721 atacctacgg tgcgattcgg ggcacggatg ttttttatcc gcagcagggc gcggggaata
     3781 cgcgttatcg tcgtagcttt gtggcgaccg gatttatgac actgataacc gtgccgctgg
     3841 cagtaattcc attttcaccg tttgtttcat ccattggttt attaacccag actggcgatt
     3901 acacgcggcg ttcgtttatt tatggcagcg ttatttgcct gctggtggcg ctggttccgg
     3961 cactcacgcg actgttttgc agtatccctt tacccgtgag tagtgcggtc atgctggttt
     4021 cttatctgcc tttactcttt tccgcgctgg tgtttagcca gcaaataacg tttaccgctc
     4081 gcaatattta tcgactcgca ttgccgttat ttgtcggcat atttttaatg gcattaccgc
     4141 ctgtgtatct gcaagacctt ccattaacgc ttcgtcctct gctcagtaac ggcttattgg
     4201 tcgggatttt actggctgtt cttatggata accttattcc gtgggaacgc atcgaataat
     4261 ttgttgaaaa aggattgata atgaagattg tcattgcgcc agactctttt aaagagagct
     4321 taagtgcaga aaaatgttgt caggcaatta aagccgggtt ttcgaccctc tttcccgatg
     4381 cgaactatat ctgtttgccg atagcggatg gcggcgaagg gacggtggat gcgatggtcg
     4441 ccgcgacggg cggcaacatc gtgacgcttg aagtctgcgg gccgatgggc gaaaaagtga
     4501 atgcttttta tggccttacc ggcgacggga aaacggcggt gattgagatg gcggcagcaa
     4561 gtggcctgat gctggtcgcg cctgaaaagc gtaatccgtt gctggcctcc agttttggta
     4621 cgggggagtt aattcgtcat gcgctggata acggcattcg ccatattatt cttggcattg
     4681 gcggcagtgc gacggtcgac ggcggtatgg gcatggcgca ggcgctcggt gtgcgtttcc
     4741 ttgatgccga cggtcaggtg ctggtggcta acggcggcaa tttagcgcgc gtggcaagca
     4801 ttgagatgga tgaatgcgat ccgcgtctgg cgaactgcca tattgaagta gcatgtgacg
     4861 ttgataaccc gctggtaggg gcacgcggcg cggcggcggt gtttggcccg caaaaagggg
     4921 caacgccgga gatggtcgaa gaacttgaac aggggctgca aaattacgcc cgtgttttac
     4981 aacagcaaac tgaaattaat gtctgccaga tggcgggcgg cggcgctgcg ggcggtatgg
     5041 ggattgcggc ggcggtattc ctcaatgcgg atattaaacc gggtattgag attgtactgc
     5101 gggcggtcaa cctggagcag gcggtgcagg gggcagcact ggtgatcacc ggggaagggc
     5161 gcattgactc gcaaacggca ggcggtaaag cgccgctggg cgttgcgtcg gtggcgaagc
     5221 agtttaatgt accggtgatt gggattgctg gcgtattggg tgatggcgtg gaagtggtgc
     5281 accagtacgg cattgatgcg gtattcagca ttttgccacg tctggcacct ttagccgaag
     5341 tgctcgccag cggtgaaacc aatctcttca acagcgcgcg aaatattgcc tgcgccatta
     5401 aaataggtca gggaattaaa aactgaccct tacctttaaa gcggatgcga tttatttcgc
     5461 atctttttta cctgaattta ctacaaaaaa agccacggta taaaccgtgg caaaatccaa
     5521 catagctaaa aataatcagg cgagtggtat gacttaaatc tctacgtcgc ggttgcaatc
     5581 tttcgagtaa atatagctga acgcttcacc acgccctacg ccataaccag cctgtaaaga
     5641 ataagcgccc ataaagatgt aatcgccttt tttcaccggg atccagttat tgtcgaggtt
     5701 ataaaccccc tgaccggaaa gaatataggc accgtgttcc tgaacgtgtg tttcgatata
     5761 accatggctg gcacctggtg caaaagagag gatatgcatg ttcatatcaa aacctaactc
     5821 tttgggcaga aaatccagca ggatcacatc atccatacct tcatagtgaa tgcgttccag
     5881 ttcgctggca ttgccagaaa ccagccacgg tgcatggcct tctaccggaa catagcggcg
     5941 cttatataaa aagatttggc tgtcttcggc ctgggcgtta acaaacgtca ttaaggagcc
     6001 tggcgggcaa taaagatagc caccttcgct taaggcaaat gttttgcctt cggctttggc
     6061 agtgatattt ccagagatca catacaggaa cgtttcaatg ccttcgccac cgaagccctg
     6121 ttggttgcca ccgttttgat gcagtgtgac cagataatcg acaaaagagg cacccagctt
     6181 tggcgtggag aggattgtcg cgtcacaatt ttcaaagccc ggaataatat tttttaccag
     6241 accgtctggt gttaacagtg cgaaattacc gtgtttcaca atcgcacggt tagccagtaa
     6301 atcatcgcgg taaccggtga cgttatttaa atatcccatt tatgactcct tatttctgcc
     6361 aggcaagttg ataaagcatg agtgccaacg ttttgacccc ttcggcaagg tcggtaatat
     6421 tggtgcgttc cgccgggtta tggctgatcc cgttgatgct gggaatgaaa atcatgcagg
     6481 tcggcacgcg aggcgcgaaa atttgtgcgt cgtgcccggc accactgtgc atcacccggt
     6541 aattcagttt ttcgctttca cacaattctg tcagggtggc gaccaggtcc tcattcatcg
     6601 gcacgggttc ttcgtccatc cataaatcga tatcaatacc aatgtccatt tcatcgcaaa
     6661 tcgcccgcat gtcgttttct aactgctggg tgaaatcgcg cagcacggcg gcgtcggtat
     6721 gacgacaatc aatggtgaac gtggttttac ccggcaccac attcaccgta ttcgggcgcg
     6781 gctctacttt cccaaaggtc agaaccagcg gatcgcccat ctttttcgct ttttcgatcg
     6841 actgatggca aatgcgactg aaagcgtaaa ccgtatcacg acgataaccc atcggcgtgg
     6901 tgcctgcatg gtttgattcg ccgttcagcg tcaccgtata gcgacgttgc ccgacaattg
     6961 cattcaccac gccaattgat tgcccattac tttccagcac acagccctgt tcgatatgca
     7021 gttcgacaaa ggctttaata tcctgacgcg gagttagcgg ggcgtccggc agagtaaatc
     7081 cgcaagcctt catcgcatcg acaaaactat ttcctttggc atcacagata ttccgcacgt
     7141 cgtcaggatt cgccagccca aagatatttt tactgcccca gaaaacatac gggaagcggc
     7201 tgccttcttc ttccgccatc gccaccactt cgaccgtacg tagcggcgcg ccgtattgcg
     7261 ttttcagcca gtcaattgcc agccaagccg ccagtgcgcc gaattgcccg tcgaggttac
     7321 cgccgttaac cacggtatcg atatgcgaac cgctcagaac cacttcctgt ggatattcgg
     7381 tgccactcag gcgaccgtat aaattcccca cttcatcgaa acgtgtttcc agcccacttg
     7441 cggccattct ttttttaaat tgctgctggg tttccagcca ttccggcgaa taaagtaaac
     7501 gggtcatccc acccgttggg tcagcgccaa atgaggaaag ccagggcagc gcttcttcta
     7561 tggcttgacg aaaatgtgta atcataagaa agtcctgtct caataattat tgcgcaaagg
     7621 gatttttcgt ttcgtatgac gtgttataaa gcgcgtcgga aattaaatac tggtaaatat
     7681 catcaacaat ttcgatgcct tcgacggcgg ctttgcgttg tttaatatcc tgatcctgtc
     7741 cgggataata aacctgaata aaaccgggcg cgggggtaat ggcatttaat tcgcgcatgg
     7801 tctggctaag atgttgacgg aataattcgc tggaggagaa aaagttcggg ttaataatta
     7861 catgtaattg ccccaaatta cgcccggcgt gtaaatcgtc atacatcgaa ctaacctgtc
     7921 gcccgaacgg taagccgagt aagacgcctg agaggacgtc aatcatcatc atcaggccat
     7981 accctttcgg cccggctgcg gggagcagag catgtaccgc gaacggatcg gttgttggtg
     8041 caccgttttt atcgaccgcc caggtatccg ggatagacat attacgcgag cgggcgtcga
     8101 gcacttttcc ccatgcctgt acggtagtcg ccatatcaaa ggtaaggatc tcgtcgcctt
     8161 ctcccggcgc ggcaaaggcc agtgggttag taccgtagta aatttccgcg ccgccaaacg
     8221 gcaccaccat tggatcggac tggcacatcg aaatgccaat taatccggcg cgggctgcct
     8281 gctgcacaaa ataagagatt gcgccgctgt gacccatccg gctgataccg accaccgcaa
     8341 cgccattttg ctgggcggtt ttgatggcat gttccatacc cattttcgcc gcgacctgtc
     8401 cggcggcatt gtcggcatgt aaaattgccg agcacggccc ggtttcctca agacgaaact
     8461 ccggttcgcg gttggtgccg ccttttgaaa tgcgttccgc gtagtattcc acgcgcaccg
     8521 cgccatgaga gtggatccct ctggcatcgg cgtaaaccaa tacttcagcc acggttgcag
     8581 cgtgctcacg ttttaaccca gcctggcaga gtttattctc aattagctgg tggagtgttt
     8641 cccgactgat tttcatctgt cttccttttt aacgacggtg tgaagcatga ctgcaattaa
     8701 catacaggga aaatatctgg attatgtgat ccagacaggc aaaaaaatat agttagaatt
     8761 tatttgataa tcttcgcgct tttaacctgg tttttaaaac aaccacgctt attaaaaaat
     8821 aacacgtaat agcatggcgg ttatttgaat tcttttgtta ataatccctg tgtgatattc
     8881 atcaccttat ttacccgttg tcatcgatac cgtaatcgcc acattaacac tgctcgtgca
     8941 attgccatgg gtgcaatttt taaggagttg ttatgatcca cgcctttatt aaaaaagggt
     9001 gttttcagga ttcggtcagt ttaatgatta tttcacgaaa actcagcgaa tcagaaaatg
     9061 ttgatgatgt ttccgtaatg atgggtacgc ccgccaataa agcgttatta gataccacag
     9121 gtttctggca tgacgatttt aataacgcca cgccgaacga tatttgcgtg gcaattcgta
     9181 gcgaagcggc ggatgcgggg atcgcgcagg cgattatgca gcagcttgaa gaggcgctaa
     9241 aacaactggc gcaggggtca ggcagcagcc aggcgttgac gcaggtgcgt cgctgggaca
     9301 gtgcctgtca gaaattaccc gatgccaatc tggcgctgat ttcagtggct ggcgagtatg
     9361 cggcggagct ggcgaatcag gcgctggatc gcaacctcaa cgtgatgatg ttctccgata
     9421 acgtcacgct ggaagatgaa atccaactta aaacccgcgc gcgggaaaaa ggcttgctgg
     9481 tgatggggcc ggactgcggt acgtcgatga ttgccggcac gccgctggct tttgctaacg
     9541 tgatgccgga aggcaacatt ggcgtcattg gcgcttccgg taccgggatt caggagctgt
     9601 gttcgcagat tgcgctggca ggggagggaa ttactcacgc gattggcctt ggcggccgcg
     9661 acctcagccg tgaagtgggc ggcatcagtg cgctgacggc gctggagatg ctcagtgcag
     9721 acgagaaaag cgaagtgctg gcatttgttt caaaaccacc tgccgaagcg gtgcgtctga
     9781 aaattgttaa tgccatgaaa gcaaccggca aaccgacggt ggcgctgttt ttaggttata
     9841 ccccggcggt gacccgcgac gagaatgtct ggtttgcctc ctcgctggat gaggccgcac
     9901 gcctggcttg cctgctttca cgcgttacgg cgcgacgtaa cgcgataacg cctgccagca
     9961 gcggatttat ttgcggtttg tataccggcg gtacgctggc tgccgaagcg gcgggattac
    10021 ttgccggaca ccttggcgtg gaagccgatg atgcccatca tcacggcatg atgctcgacg
    10081 ccgatggtca ccagattatt gacctcggcg atgatttcta caccgtcggg cgtccccatc
    10141 cgatgatcga cccaacctta cgcaaccagt taattgccga tctcggcgct aaaccgtcag
    10201 tgcgcgtatt gctacttgat gtcgtgattg gcttcggcgc gaccgccgat cctgccgcct
    10261 cgctggtaag cgcctggcaa aaagcctgtg ctgcgcgttc agacaatcaa ccactgtatg
    10321 ccattgccac ggtgacaggc actgaacgtg acccgcaatg ccgctcgcag caaatcgcca
    10381 cgctggaaga tgcggggatt gcggtcgtga gttcgctacc ggaagccacc ttgctggcgg
    10441 cagcgttaat tcatccgctc tcgcctgccg cacagcaaca cacaccgtca ttactggaaa
    10501 acgtcgccgt gattaacatc ggattacgca gttttgcgct ggagctacaa agcgccagca
    10561 aaccggttgt gcattaccaa tggtcgccag tcgccggcgg caataaaaaa ctggctcgtt
    10621 tattagaacg tttgcaataa ggggttccca tgtttacatc agtggcgcaa gccaatgctg
    10681 cggttatcga acaaattcgt cgcgctcgtc cacactggct ggatgtgcaa ccggcttctt
    10741 cacttatcag cgaactaaac gagggcaaaa cactgcttca cgccgggccg ccaatgcgct
    10801 ggcaggagat gaccggaccc atgaaagggg cgtgcgtggg cgcatgtctg ttcgaaggtt
    10861 gggcgaaaga tgaagcgcag gcgctggcaa tactggagca gggggaagtg aacttcattc
    10921 cttgtcacca tgtgaatgcc gtcgggccaa tgggcggtat tacttctgcc agtatgccga
    10981 tgctggtggt cgagaacgtg accgacggca accgggcgta ctgcaacctc aacgaaggta
    11041 tcggcaaagt gatgcgtttt ggcgcttacg gcgaagatgt cctgactcgc catcgctgga
    11101 tgcgcgatgt gttaatgcca gtattaagcg cggcgctggg gcgcatggag cgcggtatcg
    11161 atcttaccgc gatgatggcg cagggcatta cgatgggcga tgagttccat caacgcaata
    11221 ttgcttcctc agcactgtta atgcgtgcgc tggccccaca aattgctcgc ctcgatcatg
    11281 ataaacagca catcgcggaa gtgatggatt tcctcagcgt gaccgatcag ttcttcctca
    11341 acctcgcgat ggcttactgc aaggcggcga tggatgctgg cgcgatgatc cgcgcaggca
    11401 gcatcgtcac ggcaatgacc cgcaacggca atatgttcgg gattcgggta agcgggctgg
    11461 gcgaacgctg gtttactgcg cctgtaaaca ctccgcaagg tctgtttttc accggcttct
    11521 cgcaggagca ggcgaacccg gatatgggcg atagcgcgat taccgaaacc tttggtatcg
    11581 gaggtgcggc aatgatcgca gcgcctggcg taacgcgctt tgtcggtgcg ggtggcatgg
    11641 aagcggccag ggcggtatct gaagagatgg cggaaattta ccttgaacgc aatatgcagt
    11701 tgcagatccc aagctgggat ttccagggcg cgtgcctggg gctggatatt cgtcgcgtgg
    11761 tagaaaccgg cattacgcca ctcatcaata ccggtatcgc ccataaagag gcggggatcg
    11821 ggcagattgg cgcaggcact gtgcgggcac cgctggcgtg ttttgaacag gcgctggaag
    11881 cactggctga aagcatgggg attggttgag gaacgcgcaa tgacgatcat ccatcctctg
    11941 cttgccagta gtagcgcacc gaattatcgc cagtcctggc ggttagcggg agtgtggcgg
    12001 cgggcgatta acctgatgac ggaaagcggc gaactgttaa cgctgcatcg tcagggtagt
    12061 ggtttcggcc ccggaggatg ggtgcttcgc cgtgcgcaat tcgatgcgtt atgcggtgga
    12121 ttatgcggca atgaacgacc acaggttgtg gctcaaggga ttcgcctcgg gcgtttcacg
    12181 gttaaacagc cacagcgtta ttgtttgctg cgtattacgc cgcctgcgca tcctcaaccg
    12241 cttgcagctg catggatgca acgcgcggag gaaaccgggc ttttcgggcc actggtgttg
    12301 gcggcaagcg atccgctgcc tgctgaatta cgccagtttc gtcactgttt tcaggccgcg
    12361 ctcaatggcg ttaagaccga ctggcggcac tggctgggta aaggccccgg attaacgcca
    12421 agtcatgatg acacgctgag cggaatgctg ctggcggcct ggtattatgg tgctttagat
    12481 gcgcgctccg gtcgccagtt ttttgcctgt tccgacaatc ttcaactcgt taccacagcg
    12541 gtgagcgtca gttatttacg ttatgccgcg caaggatatt tcgcctcgcc actcctgcac
    12601 tttgttcatg ctctgagttg cccgaaacgt accgctgttg cgattgattc gctgctggcg
    12661 ctggggcata cgtcaggggc agatacgctg ctggggtttt ggcttggcca acaattatta
    12721 caaggaaaac catgaaaaca ctggttgtag ctcttggggg caacgcctta ctccagcgcg
    12781 gtgaggcgct gacggcagaa aatcaatatc gcaatatcgc cagtgctgta cccgcgctgg
    12841 cgcgcctggc ccgttcttat cggctggcga ttgttcacgg caacgggccg caggtggggt
    12901 tgctggcatt gcagaatctg gcgtggaaag aggtagaacc gtatccgctg gatgtgctgg
    12961 tggcagaaag tcaggggatg attggctata tgctggcgca gagtttgagc gcacagccgc
    13021 agatgccgcc tgtcacgacg gtgctgacgc gcattgaagt ttcgcctgat gatccggcgt
    13081 ttttgcagcc agagaaattt attggtccgg tttatcagcc agaagaacaa gaggcactgg
    13141 aagcggctta cggctggcag atgaaacgtg atggtaaata tttgcgccgg gtggtggcgt
    13201 ctccgcaacc gcgtaaaatt ctcgacagcg aagccatcga gttgttgctc aaagaggggc
    13261 atgtggtgat ttgcagtggc ggcggcggtg tgcctgtgac ggatgacgga gcagggagtg
    13321 aagcagtgat tgataaagat ctcgccgctg cgttgctcgc cgagcaaatt aacgccgatg
    13381 gactggtgat cctcaccgat gctgatgcgg tatatgaaaa ctggggaacg ccgcagcaac
    13441 gtgccattcg ccatgccaca ccggatgagt tagcgccatt tgccaaagcc gatggttcga
    13501 tggggccgaa ggtaacggcg gtgagtggtt atgtcagaag ccgtggtaaa cccgcgtgga
    13561 ttggggcgtt atcgcgaatt gaagagacgc tggcgggcga agcggggacc tgtatttcgc
    13621 tgtagttgta ggcattagac atttgtgcct gatgcgacgc ttgacgcgtc ttatcaggcc
    13681 tacaattggt gccgcatccg gcaattggcg cacaatgcct gatgcgacgc ttggcgcgtc
    13741 ttatcaggcc tacaatgggt accggatcgg tgtaggccgg ataaggcgtt cacgccgcat
    13801 ccggcaatca aatgagcacc aggttaactg aacttactct gtgcccacat cacgccgctg
    13861 gcatattccg gcggcagcag cgggattaag gcttccagcg tcgcggtcag acgcgatgtg
    13921 tcgctgtcgg tcaaattcag atgccccact ttacggcccg gacggacttc tttgtcgtac
    13981 cagtgcagat gcaccagcgg cagtttcagc cagtcataat tcacatcgct accaatcagg
    14041 ttgatcatca ctgacggact attcactatc ggttgcggta acggcagatc ggtaatcgcc
    14101 cgcagatgca gctcaaactg gctgatgctg gcaccgtttt gtgtccagtg accgctgtta
    14161 tgcacacgcg gtgccagttc gttgatcaac agaccttgcg gggtgacaaa acactccatc
    14221 gccatcacgc ccacatagcc cagctcctgc ataatcgccg aaagcatctc ttcggcttgc
    14281 gcttgctgct gtgcattggc ctgcgggaaa gcgacgctgg tgcgcaaaat accgtcctga
    14341 tgcaggttat gcgtcagcgg ataaaacacg gtgctgccat caaagccgcg cgcgccaacc
    14401 agcgatactt caccagagaa gttaatgcct tgctcgacaa tacattcgcc gtaacactct
    14461 tccggtaact gttcggtttc atcggcgcgt aaacgccatt gaccgcgtcc gtcatagcca
    14521 ccagtgcgac gcttaacaat cgccagctca cctaaacgat caaacaccgc aggccactcg
    14581 ctgcgatcgg caagtaactg ccacggtgcg gtcggcaggt ggagcttatc gaaaagctgc
    14641 ttctgagtca gacggtcagc aataatcggg aacacatcgc ggttcacaaa ggcaggatgg
    14701 cgcgccagct cgcgggttaa tgcggtttcc ggccagcgtt cgatctcagc ggtaatcacg
    14761 ctttgttgaa aaggcaccgc cgccggttca gcgtccagcc cgaccggcca gacagcaatg
    14821 cctaacggtt cgcctgcctg acgcagcata cggcctaact gcccgttccc gaggacgcaa
    14881 acctgtttca tgccgcacct cgcgggtccg ggttttccag cacttcgtcg gtctgggctt
    14941 tgcgccagtc attcagacgc tggtgcagtt ctttatcatg agtcgcaaga atttgtgctg
    15001 ccagtaacgc cgcgtttgcc gcgccagctt taccaatcgc cagcgtaccc accggaatgc
    15061 cgcgcggcat ttgtacgatg gagtagaggc tatcgacacc gctcagtgcg gcgctctgta
    15121 ctggcacgcc cagcactggc accagcgttt tggcggcaat catacccggc aggtgcgctg
    15181 cgccgcctgc gcccgcaata atcacctgat aaccgttctc ttcggcactt tcggcgaagc
    15241 tgaatagttt atcgggggtg cggtgagcag aaaccacttc aacgtggtgc gggacattca
    15301 ggatttcgaa gatttcggcg gcgaactgca tggtagccca gtcgcttttg gaccccatca
    15361 cgatggcgac acgcgccgga ttattgcggg aagacatgcg tcttaaaact cctgtggtgc
    15421 acaactctcg gctttagagg gcacagagaa tagcacggaa agagagcaag gaaaacggtt
    15481 gcgtggctgt gaaatcagca aagttgcggg ttttttaaaa cggaaaatga atcagctcaa
    15541 cgtcatccgc cgtgactttc accatcgaac cttctgtatg ccaggcaccg agtaccacgc
    15601 gaaaagcagg ttgctggttg gcgataagtt catgtaccgc cgggcgatgg gtatgcccgt
    15661 gaatcagcca ttgcacttga tgtttttcca tcgcactgac caccgcgttt tggttaacgt
    15721 ccatgatcgc cagcgattta ctgctgttgg cttctttact gttcgcgcgc attcgcgcgg
    15781 caatgcgttt gcgcacaaac aacggcaggg cgaggaatag cgtctgcagc cagggtttgt
    15841 ggaccttggc gcgaaaagcc tgataacccg cgtcatcggt gcacagcgtg tcgccatgca
    15901 taatcaacac ccgacgacca taaagttcga gcaccttttc ttccggcaat aacgtcatgc
    15961 cgctttcacg ggcaaagcgt ttgccgagca gaaaatcacg gttgccatga atgaaataac
    16021 agggaacgcc ggaatcggac accgctttga tcgccgccgc catctggcga tggagtgggt
    16081 tgggatcgtc gtcgccaatc cacgcttcaa acagatcgcc aagaatatac agcgcgtcgg
    16141 cattgcgggc ttcccccgct aaaaaacgca gaaaaccggc ggtgatcgcc ggttcttcca
    16201 cgcagagatg aagatctgca ataaagagtg tcgccacgat tactcgctaa cggtcacgct
    16261 ttcaatgata acgtcttctt ttggcacgtc ctggtgcata ccgctacgac cggttgcaac
    16321 acctttgatt ttgtctacca cgtccatgcc ttcaaccact tcagcaaaca cgcagtagcc
    16381 ccaaccttgc aggctttcgc cagagaagtt caggaagtcg ttatcaacca cgttgatgaa
    16441 gaactgtgca gttgcagagt gcggagcctg agtacgggcc attgccagcg taccacgggt
    16501 atttttcaga ccgttgttgg cttcgttttt gatcggttct ttggtggctt tttgtttcat
    16561 gcccggttca aaaccgccgc cctgaatcat aaagccgttg ataacacggt ggaaaatggt
    16621 gttgttgtaa aaaccttcgc ggcagtagtc caggaagttt ttaactgttt caggtgcttt
    16681 atcgtcaaaa gttttgatga caatatcgcc gtgattggtg tggaaagtaa ccatttttgc
    16741 atcctgttcc gtttgattgg tgcttcaacc cagttcgggt catatatagg gtggtgttat
    16801 agcataaccg cacgatcgga tcatcacgca atgtatgctg attcgcgcgg gaaatatggg
    16861 tattatacgc aactcaatta cccacacatg tctaaacgga atcttcgatg ctaaaaatct
    16921 tcaatactct gacacgccaa aaagaggaat ttaagcctat tcacgccggg gaagtcggca
    16981 tgtacgtgtg tggaatcacc gtttacgatc tctgtcatat cggtcacggg cgtacctttg
    17041 ttgcttttga cgtggttgcg cgctatctgc gtttcctcgg ctataagctg aagtatgtgc
    17101 gcaacattac cgatatcgac gacaaaatca tcaaacgcgc caatgaaaat ggcgaaagct
    17161 ttgtggcgct ggtggatcgc atgatcgccg aaatgcacaa agattttgat gcgttgaaca
    17221 ttctgcgccc ggatatggag ccgcgcgcga cgcaccatat cgcagaaatt attgaactta
    17281 ctgaacaact gatcgccaaa ggtcacgctt atgtggcgga caacggcgac gtgatgttcg
    17341 acgtcccgac cgatccgact tatggcgtgc tgtcgcgtca ggatctcgac cagctgcagg
    17401 caggtgcgcg cgttgacgtg gtcgacgaca aacgcaaccc gatggacttc gttctgtgga
    17461 agatgtcgaa agagggcgaa ccgagctggc cgtctccgtg gggcgcgggc cgtcctggct
    17521 ggcacattga atgttcggca atgaactgca agcagctggg taaccacttt gatatccacg
    17581 gcggcggttc agacctgatg ttcccgcacc acgagaacga aatcgcgcag tccacctgtg
    17641 cccatgatgg ccagtatgtg aactactgga tgcactccgg aatggtgatg gttgaccgcg
    17701 agaaaatgtc caaatcgctg ggtaacttct ttaccgttcg cgatgtgctg aaatactacg
    17761 acgccgaaac cgtgcgttac ttcctgatgt cgggccacta tcgcagccag ttgaactaca
    17821 gcgaagagaa cctgaagcag gcgcgtgcgg cgctggagcg tctctacact gcgctgcgcg
    17881 gcacagataa aaccgttgcg cctgccggtg gcgaagcgtt tgaagcgcgc tttattgaag
    17941 cgatggacga cgatttcaac accccggaag cctattccgt gctgtttgat atggcgcgtg
    18001 aagtaaaccg tctgaaagca gaagatatgg cagcggcgaa tgcaatggca tctcacctgc
    18061 gtaaactttc cgctgtattg ggcctgctgg agcaagaacc ggaagcgttc ctgcaaagcg
    18121 gtgcgcaggc agacgacagc gaagtggctg agattgaagc gttaattcaa cagcgtctgg
    18181 atgcccgtaa agcgaaagac tgggcggcgg cagatgcggc acgtgaccgt cttaacgaga
    18241 tggggatcgt gctggaagat ggcccgcaag gaaccacctg gcgtcgtaag taattgcgct
    18301 attgccggat gcgagttttc gcatccggtt atcgcctgcg ccaccacaac attcccataa
    18361 gcaccatccc cggcaaccac acccacatca attcagaaat aatcacctga tgcccgtacg
    18421 ctgtggtgta acgagacaat gcaaacggcg cgactttaat cacctgccag ggagcgaaaa
    18481 agcgttcatc tgaccacggc cacagccagc caacaccctt accaccagtg gttaccgaat
    18541 ccagcatgct gtgcgatagc aacgagacgg ttaaaaacag ccagcagcga atcagcccag
    18601 ccctgaacca tcggcgtcca atcagcacac ataacagcgg gacaacaaac gcaaacacca
    18661 gcgaatgggt aaacccgcga tgaccaaaaa cattgccgta agcaacgcca aatttaaacg
    18721 acaatacgtc tgcgtcgggc agcatcgcca ggatgattcc ggcaaataac agacgcggag
    18781 ggatgacttt cgaacccaac cctaaaccaa tgcatagggg aacggcggcg tgcgtaataa
    18841 cggttggcat gatggtcgct tcggcaaaat gtcgatgcta tcagcatgga tgaacggggc
    18901 gtagagggca aaagtctgaa aagagaaccg gcctgttgat acaggccggg aaagggatca
    18961 ggcaacaacc tgtacgctgt gacctgcaaa actcactgtc tgaccggcga cgattttgca
    19021 gcgtttgcgc gtttcaaccg caccgtcgac tttcacctgg ccttcggcaa tcgcgatttt
    19081 cgcctgcgcg ccgctttcgc tccagccttc cagtttcagc aagtcgcaca gttcaacgtg
    19141 cggatgttta cctaaagaaa atgtcgccat gttactcacc ctgtggatca tgatattcaa
    19201 cgcacgcctg aagcgtgttt tcaatcagcg tggcaaccgt catcgggcca acgccgcccg
    19261 gaacaggcgt aatgtatgag gcgcgtttag ccgcgtcttc aaacacgacg tcgcccacaa
    19321 ctttgccatt ttccagacgg ttgatgccga catcaatcac aattgcgcct tctttgatcc
    19381 agtcaccagg aataaagccc ggcttgccga cggcaacgat cagtagatcg gcattttcta
    19441 cgtgatgacg cagattttta gtgaagcggt gagtcacggt agtggtgcaa cctgcgagca
    19501 acagttccat gctcattgga cgaccgacga tattcgatgc gccaatcacc acggcgttga
    19561 ggccgaaggt atcaatgttg taacgctcaa gcagcgtgac gataccgcgc ggggtgcagg
    19621 gacgcagacg cggcgcgcgc tggcacagac gaccgacgtt ataaggatgg aaaccgtcca
    19681 cgtctttgtc cggatgaata cgttccagca ctttgacgtt atcaataccc gccggtaacg
    19741 gcagttgaac cagaatgcca tcgatggtgt tgtcggcatt cagcgcatcg ataagctcca
    19801 acagctccgc ttcgctggtg gtttccggga ggtcataaga gcgggagacg aacccgactt
    19861 cttcacaagc cttgcgtttg cttgcgacat aaatttgcga tgcagggtta ctacccacca
    19921 gcacaacggc cagtcctggt gcccgcagtc cggctgcaat acgcgcctga actttttgag
    19981 caacttcaga gcgcacctgc tgcgcaatcg ttttaccgtc aataatcttt gctgccatca
    20041 gagagaggat tccatctgtt acgtaggtcg aaggggatgc gcctattttg tcagaagcgg
    20101 ggcgcgctgt caggtttcgt ttcagattta tcgcgtgaag cgacctcttg cgaaggtgag
    20161 gcgcaccgtc gctgaggtcg ctgagattga aagcttcatt tttcgtccat gatggcgttg
    20221 taaatctgga actgatttat ttccttgttt aaggattaag ataatttaag aaatacctga
    20281 caatataaaa agaattttca gcctggtaat ttaccgcttc aggtctatat ttgtgttgaa
    20341 tatattttgc gcggaagtat tcatctaacg gggc
//
LOCUS       DAZUCE010000075        20172 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZUCE010000075 DAZUCE010000000
VERSION     DAZUCE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.075"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(206..952)
                     /gene="cutC"
                     /locus_tag="ACHY03_002302"
     CDS             complement(206..952)
                     /gene="cutC"
                     /locus_tag="ACHY03_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFS3905523.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MSRMEKIMTAAGPLAVTFHRAFDMCANPLNTLNNLAELGIVRVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(966..1532)
                     /gene="yecM"
                     /locus_tag="ACHY03_002303"
     CDS             complement(966..1532)
                     /gene="yecM"
                     /locus_tag="ACHY03_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS3905524.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1748..3481
                     /gene="argS"
                     /locus_tag="ACHY03_002304"
     CDS             1748..3481
                     /gene="argS"
                     /locus_tag="ACHY03_002304"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFS3905525.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3523..3567)
                     /locus_tag="ACHY03_002305"
     CDS             complement(3523..3567)
                     /locus_tag="ACHY03_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905526.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            3658..4146
                     /gene="yecT"
                     /locus_tag="ACHY03_002306"
     CDS             3658..4146
                     /gene="yecT"
                     /locus_tag="ACHY03_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFS3905527.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4268..4660)
                     /gene="flhe"
                     /locus_tag="ACHY03_002307"
     CDS             complement(4268..4660)
                     /gene="flhe"
                     /locus_tag="ACHY03_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFS3905528.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4660..6738)
                     /gene="flhA"
                     /locus_tag="ACHY03_002308"
     CDS             complement(4660..6738)
                     /gene="flhA"
                     /locus_tag="ACHY03_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFS3905529.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATIDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6731..7879)
                     /gene="flhB"
                     /locus_tag="ACHY03_002309"
     CDS             complement(6731..7879)
                     /gene="flhB"
                     /locus_tag="ACHY03_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFS3905530.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLSGIKRMFSAQTGAELLKAILKTILVGSVAG
                     FFLWHHWPQMMRLMAESQITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8081..8725)
                     /gene="cheZ"
                     /locus_tag="ACHY03_002310"
     CDS             complement(8081..8725)
                     /gene="cheZ"
                     /locus_tag="ACHY03_002310"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFS3905531.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8736..9125)
                     /gene="cheY"
                     /locus_tag="ACHY03_002311"
     CDS             complement(8736..9125)
                     /gene="cheY"
                     /locus_tag="ACHY03_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFS3905532.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9140..10189)
                     /gene="cheB"
                     /locus_tag="ACHY03_002312"
     CDS             complement(9140..10189)
                     /gene="cheB"
                     /locus_tag="ACHY03_002312"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFS3905533.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10192..11052)
                     /gene="cheR"
                     /locus_tag="ACHY03_002313"
     CDS             complement(10192..11052)
                     /gene="cheR"
                     /locus_tag="ACHY03_002313"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFS3905534.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKSDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11071..12672)
                     /gene="tap"
                     /locus_tag="ACHY03_002314"
     CDS             complement(11071..12672)
                     /gene="tap"
                     /locus_tag="ACHY03_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFS3905535.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRVLQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12718..14379)
                     /gene="tar"
                     /locus_tag="ACHY03_002315"
     CDS             complement(12718..14379)
                     /gene="tar"
                     /locus_tag="ACHY03_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFS3905536.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14524..15027)
                     /gene="cheW"
                     /locus_tag="ACHY03_002316"
     CDS             complement(14524..15027)
                     /gene="cheW"
                     /locus_tag="ACHY03_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFS3905537.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15048..17012)
                     /gene="cheA"
                     /locus_tag="ACHY03_002317"
     CDS             complement(15048..17012)
                     /gene="cheA"
                     /locus_tag="ACHY03_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFS3905538.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFGVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVADAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17017..17943)
                     /gene="motB"
                     /locus_tag="ACHY03_002318"
     CDS             complement(17017..17943)
                     /gene="motB"
                     /locus_tag="ACHY03_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFS3905539.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17940..18827)
                     /gene="motA"
                     /locus_tag="ACHY03_002319"
     CDS             complement(17940..18827)
                     /gene="motA"
                     /locus_tag="ACHY03_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFS3905540.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18954..19532)
                     /gene="flhC"
                     /locus_tag="ACHY03_002320"
     CDS             complement(18954..19532)
                     /gene="flhC"
                     /locus_tag="ACHY03_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFS3905541.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19535..19885)
                     /gene="flhD"
                     /locus_tag="ACHY03_002321"
     CDS             complement(19535..19885)
                     /gene="flhD"
                     /locus_tag="ACHY03_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFS3905542.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4831 a         5565 c         4923 g         4853 t
ORIGIN      
        1 cagttttagc aatttaaatc acataattaa tgtgaatata taaaatattt ttagcacaac
       61 gctatgtgca ctgtcaccca ctcaacagat cgcggtaatc gttcattttt tatactattc
      121 agggataaaa aaaggcccct gttgaaattg caggggcctg gtacgagcaa gcatcatatt
      181 gggcgacatg atgcaacggt aaaaatcatt tggcctgatg gcgttcaatg attcctttca
      241 tttcagcaac cgccgccccg tctacgacat aacgcgaata ctcgtccgcg tgtgcatctg
      301 atgacatgga caatccttga ttacgataac gcatcggtga ggcttgccac gctcccgcgg
      361 agctatggac ttccagcact ccggcatcga ggaagtggtg caggttttct gcacggactc
      421 ctgctccggc cataatgatt ggagcatcac gatgggcaat aagttccata atttttgata
      481 aaccttgcag tgcgtctgat ttttgccctg atgtcagcac ccggacaatg cccaattccg
      541 caagattatt gagtgtattt agagggttag cgcacatatc aaaggcgcga tgaaaagtca
      601 ctgccagcgg accggcagca gtcattattt tttccattcg tgacatatcg acattaccgt
      661 caacatcgag cacgcctgtc accagtccag gaaaacctaa ttcgcggact gtgcgcacat
      721 cctcaagaat ggcggcaaac tcaccgtcgc tgtagcaaaa atcaccaccg cgtgggcgga
      781 taatcggatg cacagggatc gtcacccgct ggcgcacgga tttcagtaca cccaacgacg
      841 gcgttaagcc cccctctttt ggggctgcgc ataattcaac tctgtctgcg ccgttttgct
      901 gcgccgttag tgcacattcc atgctgtaac agcaaatttc cagtaatgcc atttttactc
      961 cttaattacg ccgactgctc gctggcaacg atctcttcaa tggaccacgg atgaaactta
     1021 atagttgttt tgccatcggt gaccgccagc gtcggattcg gtaatcgctc atgctcacct
     1081 tttggcgaac tggttttaac tgagatcccc ggtaggctga gtccttcatc agaaagcaat
     1141 gccagcgcac gggcattcag cgtttctgga tcacccggca gaacgatttc aatatgttcc
     1201 caaccttcgt gcgggtaacg tttttccccg ggccacggta gctccacaat ggaaaactgc
     1261 cagtgagcaa cctgtaccgg ttcatgcaat ttaaacagac aaatcggtct gccattgatc
     1321 atattttctg acaaaagctc gccgcactgt tcaaacccgc gacgccagcg ttcagcggtg
     1381 gcgttttgat ggcaacgcaa ggaaatgtga tcggcagtca acggagtgat atccaacccc
     1441 aaacggcggg aaagttcatc taatgcgtgg gtaaatcgcg gtaaatccga tgcaatatcc
     1501 tgcagctcgt cgatagattg ccagttcgcc ataatcactc ttcgtctttc agtaaaagcg
     1561 ttaatttacc ctgttgccct gcgccaacca accgctgatt tcacgccgct tctgatgcaa
     1621 tagtgaaaac ggcaatacgg cacgcgcacg ttgctgacga aaacagccat ttgcagtata
     1681 ctcccgccct aatttcttta actggtgcgg gcaatttttg ctcgcttcat caatgtaagg
     1741 tattccggtg aatattcagg ctcttctctc agaaaaagtc cgtcaggcca tgattgcggc
     1801 aggcgcgcct gcggattgcg aaccgcaggt tcgtcagtca gcaaaagttc agttcggcga
     1861 ctatcaggct aacggcatga tggcagttgc taaaaaactg ggtatggcac cgcgacaatt
     1921 agcagagcag gtgctgactc atctggatct taacggtatc gccagcaaag ttgagatcgc
     1981 cggtccaggc tttatcaaca ttttccttga tccggcattc ctggctgaac atgttcagca
     2041 ggcgctggcg tccgatcgtc tcggtgttgc tacgccagaa aaacagacca ttgtggttga
     2101 ctactctgcg ccaaacgtgg cgaaagagat gcacgtcggt cacctgcgct ctaccattat
     2161 tggtgacgca gcagtgcgta ctctggagtt cctcggtcac aaagtgattc gcgcaaacca
     2221 cgtcggcgac tggggcactc agttcggtat gctgattgca tggctggaaa agcagcagca
     2281 ggaaaacgcc ggtgaaatgg agctggctga ccttgaaggt ttctaccgcg atgcgaaaaa
     2341 gcattacgac gaagatgaag agtttgccga gcgtgcacgt aactacgtgg taaaactgca
     2401 aagcggtgac gaatatttcc gcgagatgtg gcgcaaactg gtcgacatca ccatgacgca
     2461 gaaccagatc acctacgatc gtctcaacgt gacgctgacc cgtgatgacg tgatgggcga
     2521 aagcctctac aacccaatgc tgccaggaat tgtggcggat ctcaaagcca aaggtctggc
     2581 agtagaaagc gaaggggcga ccgtcgtatt ccttgatgag tttaaaaaca aggaaggcga
     2641 gccgatgggc gtgatcattc agaagaaaga tggcggctat ctctacacca ccactgatat
     2701 cgcctgtgcg aaatatcgtt atgaaacact gcatgccgat cgcgtgctgt attacatcga
     2761 ctcccgtcag catcaacacc tgatgcaggc atgggcgatc gtccgtaaag caggctatgt
     2821 accggaatcc gtaccgctgg aacaccacat gttcggcatg atgctgggta aagacggcaa
     2881 accgttcaaa acccgcgcgg gtggtacagt gaaactggcc gatctgctgg atgaagccct
     2941 ggaacgtgca cgccgtctgg tggcagaaaa gaacccggat atgccagccg acgagctgga
     3001 aaaactggct aacgcggttg gtattggtgc ggtgaaatat gcggatctct ccaaaaaccg
     3061 caccacggac tacatcttcg actgggacaa catgctggcg tttgagggta ataccgcgcc
     3121 ttacatgcag tatgcataca cgcgtgtatt gtccgtgttc cgtaaagcag aaattgacga
     3181 agagcaactg gctgcagctc cggttatcat ccgtgaagat cgtgaagcgc aactggcagc
     3241 tcgcctgctg cagtttgaag aaaccctcac cgtggttgcc cgtgaaggca cgccgcatgt
     3301 aatgtgtgct tacctgtacg atctggccgg tctgttctct ggcttctacg agcactgccc
     3361 gatcctcagc gcagaaaacg aagaagtgcg taacagccgt ctaaaactgg cacaactgac
     3421 ggcgaagacg ctgaagctgg gtctggatac gctgggtatt gagactgtag agcgtatgta
     3481 atcgattttt cgtgagagtg aagcctgatc agggttagcc gatcaggctt ttttattgcc
     3541 atctaaatgt attctgaaaa cggacatgcc atcgttttct cactgttgag taagaggcca
     3601 gaagcgtaat atccaggccc aggaaaacga taacggttca atttaaggaa taccgcagtg
     3661 tttaaatttc ttgtattaac attaggcatt atctcttgcc aggcttacgc agaagatacg
     3721 gttatagtaa atgatcatga catttcagcc atcaaagatt gttggcaaaa aaattcagat
     3781 gatgatactg acattaacgt gatcaaatca tgtctgcgac aagaatacaa tctcgtcgat
     3841 gcgcaattaa ataaagctta tggtgaagct tatcgttata tagaacaagt gccacgcaca
     3901 ggtgcaaaaa aacctgatac cgaacaactt aacttgctta aaaaatcaca acgagcctgg
     3961 ctggatttta gagacaaaga atgtgaatta atcctttcaa atgaggatgt tcaggattta
     4021 agtgaccctt attctgaatc agaatggcta tcatgtatga tcatacagac caatacgcga
     4081 actcgccagt tgcagctata ccgtaactct gaagattttt atccaagccc tttgacaaga
     4141 ggataattca catatttttg gcatgttttg ttgcaatcta ttcctgataa atgattgcaa
     4201 caaaacatcc agcctttttc actgagtcat taaacatact cgcgagcgcg taatttttat
     4261 ttgtccttca gcgataattc acaatcactt cattccgttg tacctttagc ggtggaatta
     4321 accgcccacc gccaggcact tcccagataa agcgcaacgg ttctgctgcc gctataccgg
     4381 aaaaggccac ggtggttccg ctctgcccct ctaattcgac acaacgagat tgcgaacaca
     4441 agcgaacccg cagtcctgca ggtgtcgggc cgataagctg ataacgccac gccaccagcg
     4501 tcatcaatcc tgaagcaggt tgtcgagtag aaagaggcgc tgacgacatc gactcaccgc
     4561 gatgatttag cgtaattccg acactacttg cctgccacat cccctccccg gcggcttgca
     4621 ctagcagcgg aaacaataat attgctaata aggttctcat catttaccgc caattgtcgc
     4681 cgtcatgcgg atatgtcggt tatcagatag ttccagattc gacagcacca ctaactgcgg
     4741 caagctgcgg cgcagaaagc gagacaataa tggtcgcagc gcgtggttca ccaacaatac
     4801 tggcggcgca cccaacatct cctgacggga tagcgcttcc tgagtttgcg ccagtaaacg
     4861 atccgccagc cctggctcca gtccgccccc gccctgcaac gcctgtagca acaaacgttc
     4921 cagcggtgta tcgaggccaa taacatggac ttcatctttg ccaggaaacc actgctgggt
     4981 aatcgcccgt cccaacgcca cgcgcacgac ggcggttaat tcatgtggat cactttggat
     5041 gggtgcatgt tccgccagcg tttcgagaat ggtgcgcata tcgcgaatcg gtactttttc
     5101 atcgaggaga ttttgcagca ctttatgcaa tgtggtgagc gtgacgacgc caggaacgag
     5161 atcttccgtc agctttggca tctcctgggc gacgcgatcc agcagctgtt gcgcctcctg
     5221 acgaccaaac agctctgcgg catgctggct aatgaggtgg ttaagatgcg ttgctaccac
     5281 cgtgctggcc tcaaccacgg tgtacccctg aatctgcgcc tgttctttta gcgcactttc
     5341 aatccagata gcattcaggc caaatgccgg atcgatggtc gcctcaccag gtaacgtccc
     5401 ggcagcggtt ccagggttaa tcgccagcca gcgccccgga taagcatcac cactgcctat
     5461 ctccacgcct ttcatcaaaa tgcgatagcg ggcaggttgc agatccatat tgtcgcgaat
     5521 gtgcaccacc ggcggcaaga agcccatttc ctgagcaaat ttcttacgaa tactacgaat
     5581 acggcccagc aactcgccat cttgttggaa atcaaccatc gggatcagtc gataacccac
     5641 ttccattccc agagaatctt ccagttgtac atcgttccac gtcgcttcga caacggcatt
     5701 attctctgcc atttttaccg gtttgggttc ggcaggcgct ttttgttcgc gtccgcggat
     5761 ccaccaggcc agccccagca atccagcagt gaacagcaaa aataccaggt tcggcattcc
     5821 aggcaccagg ccgagcaaac cgagcacggc ggcgcttaac aacataacgc ttgggttact
     5881 gaaaagctga ttcaccatct gctcgccaac atcctgatcg gtgctgacac gcgtaacaat
     5941 gacccccgcg gcggtagaaa tcaccagcgc cgggatttgt gccaccagac cgtcgccaat
     6001 ggtcaagagc gtataacttt ccgccgcgtg tcccatgctc atgccatgtt gcagcacgcc
     6061 gaccagcaac ccgccgacaa cgttaatgac catgatgagg atcccggcga tggcatcgcc
     6121 gcggacaaac ttacttgccc cgtccattga gccgtaaaaa tcggcttcct gagtgacttc
     6181 ggagcggcgt tttttcgcct catcttcacc aatcaatccg gcgttaaggt cggcgtcaat
     6241 cgccatctgc ttacccggca taccatcgag aacaaagcgc gcacccactt ctgcgatacg
     6301 cccggcacct ttggtaatga ccataaagtt gatgatcacg agaatgacaa acaccacgat
     6361 accgatagcg aaattgccac caacgaggaa gtgaccgaac gcttcgacca ccttcccagc
     6421 cgccgctgcg ccggtatgcc cttccatcaa aatgatacgg gttgaagcca cgttaagtgc
     6481 cagacgcaac agcgtggtaa acaacagaat ggtcggaaac gcagcaaact caagcgtgcg
     6541 ctgggtaaac atcgccacca gcaacaccat gatcgacaag gcaatattga aggtaaacaa
     6601 caggtcgagt atgaatgcgg gcagtggcag caccatcatc gacaagatca acaggatcaa
     6661 aatcggtccg gcaaggatct gccattgtgt cgatttcagg tttgcgggca ggcgcagcat
     6721 cgcggccaga ttactcatgg gtcggtttct cgttaataaa atccagggct tccggcaccg
     6781 gaagatgagt aggttgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc
     6841 agacccaggc cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa
     6901 tctccgcatg tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat
     6961 tttcagcgcc aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt
     7021 tcggtgcgct cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat
     7081 tgacaatgac atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct
     7141 gcatctgacg gatccgtcct ttaacatggg ggtcgccttc gctttgtttg aactcatcac
     7201 gaatatcctg ccgcgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt
     7261 caaatcccac cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccagat
     7321 ccatcgcatt acccatggcg gtaatctgag actcggccat taagcgcatc atctgcggcc
     7381 agtgatgcca gagaaaaaac cccgccacgc tgccaaccag gatggttttc aaaattgctt
     7441 taagtaactc cgcgccagtc tgtgccgaga acatccgttt aatgcccgat agcgggttga
     7501 gtttggaaaa cttcggctgc aaggatttgc cgctaaatac cagccctccc agcatgaccg
     7561 gagaaatgag cgccaccagc accacgccgc taatcagcgg cagcagcgcc agcatggctt
     7621 ctctgatcag cagaataatt tgcccgagga tcagattcgg gtcattgata atactgtgat
     7681 caaaatgcag cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa
     7741 accagataac actaacgccc accagcaaaa tcagcagtga agtcagttca cgggaacgcg
     7801 ggatttgccc ctcttcccgc gctttttcta gtcggtgagg tgtgggggct tctgttttgt
     7861 cgtcgctctc gtcagacacg tcgccaatcc tggaaagagt attaagcgta aatgatgcca
     7921 gagcgcaaag cgttcaatgg tttgagtaag gggcaaaaca ggcgggattt agggcttttg
     7981 ctgccacaca tcaagcatag tgtgcgtttg tcggatgcgg cgtcatcgcc ttatcagacc
     8041 gcctgatatg acgtggtcac gccacatcag gcaatacaaa tcaaaatcca agactatcca
     8101 acaaatcgtc cacctgatcc tgactggcta ccacaccggc tttgctggta tcgacctgag
     8161 gtccattaag caaactctgg ttttcacgtt ttggacgcga ctcctgttcc gggatgtttt
     8221 ccaacagcac catcagcaac tggcgttcga tctcctgaat gacatccatc atccgcttaa
     8281 taacctgtcc ggtgagatcc tgaaaatcct gcgccatcat gatttccagc agttgcgcgt
     8341 tagtaaagct ggtatgcgcg ggtacatctg ccagaaattg tcgtgtatct gttaccagtt
     8401 cacgggcgtc ggcaaggtca atcggatcgg caaaccagtc atcccaacgt tgggttaacg
     8461 cttttgctga tttctccatt tgatcctgat gcggttgtga cgcctcaaca ctgttcagcg
     8521 cccgctccgc agcctgggcg gtcatctgca caacatagta caaacgatcg cgcgcatccg
     8581 ggatggcttc cgccgcttcg gcaatggcct gatccagccc cagttcccgc aaactgtcgc
     8641 gcagcatacg cgtcaggctg ccgatgcgcg caatgatatc gccagctgaa tgctcgtcag
     8701 caggtttgat tgatggttgc atcatagtcg catcctcaca tgcccagttt ctcaaagatt
     8761 ttgttgagtt tttcctccag cgtcgcggcg gtaaatggct tcaccacata gccactggcc
     8821 cccgcttgcg ccgcagcaat gatgttctct ttcttcgctt ctgcagtcac cattaacact
     8881 ggcaatgccg acatcgcgcc atctgcacga atcgttttca gcaactccag gccatccata
     8941 ttgggcatgt tccagtcgga gataacaaat ccataaccgc ctgcctgcaa cttattgaga
     9001 gcgtcgacgc catcttccgc ttcctcaaca ttattgaatc ccagctcttt cagcaggtta
     9061 cgcactatgc gtcgcatggt ggaaaagtca tccacaacca aaaatttaag ttctttatcc
     9121 gccatttcac actcctgatt taaatacgta tcgcctgtcc ggcactaatt tttgccaaca
     9181 tttgttggct tacctggcta agatcgacca cttcgcagac accacccata ttgatggcct
     9241 cgcgcggcat gccgaacacc acgcaacttg cttcgttttg cgcaagggtc catgcccccg
     9301 cctgacgcat cgccaacatt cctgccgcgc cgtcgttgcc cataccggtc aggatcaccc
     9361 caaccgcatt acgccccgcc tgtttggcga cagaatggaa caacacatct accgaaggcc
     9421 gatgacggtt aaccgccggg ccatcgtgaa ttttgatttg ataatttgcg ccactacgcg
     9481 ccagctccat atgccgatcg cccggcgcga tataggcatg ccccggcaag acacgttctc
     9541 cgtcttcggc ttctttaacc ccgatctggc aaagcttatt aagtctgtcg gcaaaagagc
     9601 gggtgaaacc gggcggcata tgctgggtaa ttaacagtgc cgggctggaa agcggcaacg
     9661 gttgcagtac gtgacgaatt gcctcagttc cacccgttga agcaccaatc gcaatcagtt
     9721 tttcagaact cagcaacggc cccgccttca gcgttgtcgg tgccgacaat ggcttatgtg
     9781 ctgcaaggct cgcctttgct gccgtacgca ccttttcagc aatcatttcg ctatacgcca
     9841 gcataccttc gcgaataccc agttgcggtt tggtgacaaa atctatcgcc cccagctcca
     9901 gcgcgcgcag cgtaacttct gaccctttgc cggtcaggga agaaaccatc actacgggca
     9961 ttggacgcaa acgcattaat ttttcgagga aatccagtcc gtccatccgc ggcatttcaa
    10021 catccagcgt caatacatcg ggattgaatt tcttaatcaa atcacgcgca accagcggat
    10081 caggggcggt cgccaccatt tccatgtcgc tatggctgtt gatgatttct gtcatgatct
    10141 ggcgcatcag tgccgaatca tcgacagata acaccctgat tttgctcatc gttaatcctt
    10201 acttagcgca tacaccgtct gaccacgcag cgtgaagcgg cgctcaaggt ggctaaagtt
    10261 ttcagagtga cccgcaaaca gcaatccgtc ggatttaagg agcggaacaa agcggcgcaa
    10321 aatctcctgc tgggtagttt gatcgaagta gatcatgacg ttacgacaga agatcgcatc
    10381 aaacggcccc ggcacggtgt actgtttcgc cagtaaattc agcggggcaa aatcaacata
    10441 gttcgccagc tcctgacgca cgcgtaccag cccttcatgc ggtcccgtcc ctcgcatgaa
    10501 ataccgttgc agttgctgcg gcgtcaggtt tttcaactct tcatggcgat agataccgct
    10561 tctggctttt tccagcactt cggtgtcgat atcactggca aacactttcc agcgtccggg
    10621 cgcggtgccc aatgtgtcag ccagcgtcat cgcaatgctg tacggctctt cgccggtcga
    10681 agccgccgcg ctccatacgc gatactcgcc agaacggcga cgtgcgtgat ccgcgagcag
    10741 agggaaatga tgtgcctcac ggaaaaatgc cgtcagattc gtggtcagcg aattgataaa
    10801 cgcctgccac tcaccgctgt gctgattaga ttccagcaag ttcagataat gaccgaaatc
    10861 cgtcagtccc agcgaacgca aacgacgaac cagtcggttg taaaccatgt cgcgtttatg
    10921 gtcagccaga acgatcccgg ctcgttgata gatcaattga cttatccgcc gaaaatgcgc
    10981 gtcggaaagc gccaggcgct cggtcatctg taacaataaa gacgtttgcc cacagggcag
    11041 agatgaagtc atagcgcctt ctcaatcact tcaggatacc actggcgcaa tttgtaactg
    11101 cgccgactca tgtcgtgcta cttcatgttc ttcaagggta aataccgcca cacgcgacga
    11161 aagatggtcg gcctgattcg ccagttgttc cgttgccacc gccgcttctt ctaccagcga
    11221 ggcgttctgc tgagtcacct gatccatctg gctgacagcc tgtgcaacct gctcaatccc
    11281 ccgccgttgt tcttccgacg ccgaagcaat ttctcccata atgtcgttca cgcgggtcac
    11341 cgaactgaca atatcggtca tggtcgcggc ggcgttattc accagtttcg aaccctgctg
    11401 gacacgattg actgactctt cgatcagccc tttgatctct tttgctgcct gcgcgctacg
    11461 gctggcaaga ttgcgcactt cacctgccac taccgcaaaa ccacgcccct gctctccggc
    11521 gcgagccgct tccactgccg cattcagggc cagaatatta gtctggaaag caattccgtc
    11581 gataacgctg ataatgtcgc caattttttg cgagctggtg gcgatctcct gcatggtgtg
    11641 agtcatggta ctgacctgaa caccgcccgc ctgcgccgtt gtcgcggcat tttttgccag
    11701 ttccgacgcc tgtcgtgcgt tatcggcgtt ttgccctacc gtggcggtta attgctccat
    11761 actggcggcc gtttgtgcca gcgatgccgc ctgctgttcg gtacgacttg agagatcgtt
    11821 attgcctgcg acaatctccg cgatcccaat gtgcatctcc tggcttccct tacgcacatc
    11881 actcaccgtc ccacgcaaag cctgctgcat ggtcttcagg ctggcaaaaa tggcggtgat
    11941 ctcattacga ccatataccg caatcggacg cgccagatta cccgcagcaa tgctgtcaaa
    12001 atggctaccg ataatggcca gtggttgaac aatcatcttg cgcgtccacc acagcgcact
    12061 gctgatgtag atcgccgcaa caataatcat gctgataaac accagcgccg aaatctgata
    12121 gttacgctgg ctttgcgcac tggcggcttc cagcacatgg ttgatctcca gctgccaggc
    12181 ctcaaagtta acgtcaaacg cattctgtga tccctgcacc ggcgcagtga ggaaatccga
    12241 aagttgatta ctttcaagcc aggtcgcctg atgttcgaga tcgttgtgcc agcgggcaaa
    12301 acttttctcc gtctcttttt gcaataccct gacgtgctcg ttgcccgcag tcatcgccat
    12361 aaaactttta aacagcgtgg tggattgcgt cagactggcg cgcgccgtcg tcatcaacgt
    12421 tttaatgtca tccgccggat agctaagcgc tgtcagagtg cctgctttgt tcagcgcggt
    12481 actggcctgt aacattaccg cccgagtttg cgctaatgcc gcacgttgct gattgctctg
    12541 ctcgacctga ttcagtcgtt gcaaatcgtc gcgaaatgcc caaaaagaca tgccgttact
    12601 gccaatctgc aagatcccgc agagaatcaa aattaaaaac agcgtggtcg aaattcgaat
    12661 acgattaaac atcaacgctc cccatcaggc ggcaatgacc gcgttagtaa atactcgtca
    12721 aaatgtttcc cagtttggat cttgttcagc aattcgcagt cgtggctgtg ccggtggttg
    12781 ctcactggca ggacgggatg gtgtttgcgg tttattggtg agtgggctgg ctgccagacg
    12841 aaacgcggaa accgcttgcg ttaaacgact agcctgttct tccagcgcag ctgcagcggc
    12901 agctgattcc tgcaccagcg atgcgttctg ttgcgtgacg cgatccattt ccgaaaccgc
    12961 caatgcgact tgatcgatgc cacggctctg ttcatccgat gccgatgcaa tctcgcccat
    13021 aatgtcagtc acgcgagtga cagcattgac gatattgttc attgtttccc cggcgctttc
    13081 gaccagcacc gaaccggtat caacgcgcga gacggagtct tcaatgagag ctttgatctc
    13141 ttttgccgcc tgggcgctac gactggcaag attacgcact tcgcccgcca ccacggcaaa
    13201 accacggccc tgttcacccg ctcgcgcggc ttcaaccgcg gcattcagcg cgaggatatt
    13261 agtctggaag gcaataccgt cgataacgct gataatgtcg gcaattttct tcgaactatc
    13321 ggcgatctca tgcatcgttt tcactacgcc atccaccact ttgccgccgt gctgggcggt
    13381 gtcggaggca ctttgcgcca gttgcgaggc ctggcgggcg ttatcggcgt tttgcttcac
    13441 tgtcgcggtg agctgctcca tgctggcggc agtttcttcc agcgcggatg cctgctgttc
    13501 agtacgggag gaaagatcgg tgttgcccgc cgcaatttca cgggtaccgg catagatggc
    13561 atctgaacct tcgcggacat gagtgacggt gtcagtcaaa gagcgttgca tatgtgaaac
    13621 gctctgcgcc aggtcgccca tttcactgcg cccgtcaatg gtcagggtat tcgccaggtt
    13681 accaccggcg atttcgcgaa tgtgagcaat aatttttgcc agcggtgtaa gcaacatacg
    13741 gcgaatgccg taccacgcta ccagcagaat caataccacc accagcgcga taaccgccag
    13801 ttgccactgg gcaaatcggt aatcatctgc gttgtcagtg acgatatcgc gatacagttt
    13861 ttcactgctg agggcgtact gagcaaacgc ttcgcccatt gcattttgca ttccctgggt
    13921 tggctgagcg aaataagctc cagtattgcc ataatcaaga taatcaatca gctcagttaa
    13981 cgctgtgtga tagtttttat atttttcatc aatattacga ctggtagcga ccatttcagg
    14041 taacggtgcc atgcttttga attttttata atgagtcgcg gcctgggcca atgttttcct
    14101 ggcgctatcg agcaattcaa ctttggcatt actttgttga ttggaggaat ccatcatcat
    14161 ccgcaccgct gaacgactca ggttaatgcg cgtttgcagc attaaatccc aggttgacgt
    14221 cagctcgccc tgctgttccc gtaattgatt ggaaaccaca aagctcttct ggctatggtg
    14281 aagggaagaa aaaaacagac tgccggaaat aagctgtaac agtgcgaata cccccagcac
    14341 cattaccaac agcgtgacta cgcggatacg gttaatcata aggcaccttc ctgaaaacaa
    14401 gttgatctcg ttatcggcaa ggaaggggga aactttattg ctgatgccac ccgccgcgaa
    14461 attgaaataa aaaacccgat gcgcagatca tcgggttcat ttcaattgag gaaatcggga
    14521 gaattacgcc acttctgacg ccgcgctatc taacagcgcc atctcttcgc tgttcagcag
    14581 tttttcgatg ttcaccagaa tcaacatccg gtcgcccagt gcgcccagtc cagtgagata
    14641 ttctgttgaa agcgtcacgg caaattccgg tgccggacga atttgctccg ccgtcaatga
    14701 aagcacatct gaaacgccgt caaccacgat gccgaccacc cgctgtccga gattcaggac
    14761 gataactacc gtgttgtcgt tatagtccac atccacctgg ctgaacttaa ttcgtaagtc
    14821 aacaatcggc acaataacgc cgcgcagatt cgtgacgcct ttgataaacg ctggcgtgtt
    14881 cgcaatccgt gttacctgat cgtagccacg gatctcctgc actttcagga tatcaatacc
    14941 gtactcttca tcaccaaggg taaataccag aaattcctgg cctgacggct cgctggccag
    15001 ctttgttaca ttcgtcatac cggtcatatt gttacctttt tactcattca ggcggcggtg
    15061 ttcgccatac gttgttcgcg gtttatcgcc tgcaaggcgg agacatcaac aatcagtgcc
    15121 acgctgccgt cgccaagaat ggtcgcagca gaaatgccgg ggactttgcg atagttactt
    15181 tcaaggtttt taaccacaac ctggtgttga ccaattaatt gatccaccag caaggcgtag
    15241 cggcgaccgc cactttgtaa gatcaccaca attccctggg tggcttcggt tttcgcgtcc
    15301 gcgacgttga acactttcca cagttcgacg atgggcagat attcaccccg cacttccagc
    15361 acccgctcgc cgccggccag tggatggaga tcggcttcac ggggttgcag tgattccata
    15421 acagcattca gcggcagaat gaaaacttca tccgctacgc gtacggacat gccgtcgagg
    15481 atggccagcg tcagcggcag taaaatgcgg atcgtagtgc cagtaccctg cttcgactgg
    15541 atttcgacat gaccgcccat ctcctggata ttacgtttaa cgacgtccat gccgacgccg
    15601 cgcccggaga cgtcggtgac ctgctctgcc gtggagaagc ctggtgcaaa tatcagcatc
    15661 gcgacttcgt cgtcgctcat gttttcgctg acagtcaaac cttgcgaggc cgcttttgcc
    15721 agaattcgct cacggtttag ccccgcccca tcgtcggtca cttcaatgca aatgttgccg
    15781 ccctgatgtt cggcagacag aattaaattt ccgacgctgt ttttacctgc ggcgagccgt
    15841 ttttctggca gttcaatacc gtgatcgagg ctattgcgta ccaggtgggt cagcgggtcg
    15901 ataatgcgtt ctatcaggct cttgtcgagc tcggtggaac tgcccaccag cgtcagttct
    15961 acctgcttgc cgagttttcc cgccagatca cgcaccagcc ggggatagcg actaaagaca
    16021 tattccatcg gcatcatgcg aatcgacatc actgattcct gcaaatcacg ggcgttacgt
    16081 tgtaactgcc ccatgctggt aatcaaatca ccatgattaa ccgggtccag ttcgctggaa
    16141 cgctgggcaa gcatggactg ggtgataacc agctcgccga cgaggttaat taattgatca
    16201 accttttcta ccgctacacg gatgctggtg gattcactgc tgcgcgtcgt tttttcccgc
    16261 tccacgcgac cggttggcgc ttgttcggct gccagtttaa gcactggtgg ggtggatatt
    16321 tttggcgaga cttctactgt ttcaaaggta atctgatcgg cttcaatcac aaaacagagt
    16381 accgctgtga tgtcatcttc ggcgatatcg cccggtaata ttgccgagag agaatccgcc
    16441 cctttcacca cgtcagttaa cgttgtcaga tgccccagct cttcttccag caggtcgact
    16501 tcgccggcct tcaggcgcga aaggataatt cgtcgcggca actgactgcg actctgctca
    16561 tcttgcggtt cacttttggc aaccacactt aatcgggtca ctgcggatgg cgtttcgcct
    16621 ttcgcttcta atgccagttg acgcaaggcc tggcagatat aatcgaagct ggcggcatcc
    16681 ggctcttgcg actgtttata agcgtcgagc tgttcttgca tgatgtcctt cgtttccaaa
    16741 aacagattga taatgtcggt gttgagttgc atctcacctc gtctggcttc atcgagcagg
    16801 ttttccatca gatgcgtggt ttcctgcaaa acgccgaagc caaaagttcc tgcccctcct
    16861 ttgatcgagt gggcagcccg aaagatggca ttcaattgtt cggcatctgg cgcttccggc
    16921 tgcaaaacca gcagatgctg ctccatatca gccaacagtt cgtccgcttc gtcaaaaaat
    16981 gtctgataaa aatcgcttat atccatgctc acgctgtcac ctcggttcgg ctgatggcat
    17041 tgtgggaaca ctgacctgtg gtgcaacctc aggtttttcc agggcgctta ctggctcatt
    17101 ctggctttcg gcgttttcat gcaaaatggc ctgttcggct tgtttgttca gtaccagcag
    17161 gctgatgcga cggttgacgg catcatcagg tccgcgatcg cttaagcgca tcgttgccgc
    17221 catgccgacg acacgtaaca ctttgccgct atccaacccg ccgaccatca gttcgcggcg
    17281 ggatgcattg gcccgatcgg cagaaagctc ccagttgctg taacctttct caccgctggc
    17341 gtagggaaaa tcatcggtat gacctgaaag gctaatacgg ttgggaatac cgttcagtac
    17401 aggcgcgatg gcgcgcagaa tgtcgcgcat atagggttcg acatcggcac tgccggttct
    17461 aaacatcggg cgattctggc tatcgatgat ctgaatacgt agaccttcct ggaccagatc
    17521 gattttgaga tgtggacgca acgcccgcag tttcggatcg gactctatca actggtcgag
    17581 atcaccccgc aatttccgca atcgactttg ctccatgcgt tttttcaact cttcgatgtt
    17641 cggctgctta ttcacttccc cctggctttg ggtgtaatca tcaccaccgc cgggaattgg
    17701 gctttcacta ttagaaatgc gatcgccgcc cgtaaccgca gtcgccagtg gagtccggaa
    17761 gtactccgca atctgaatca actcttttgg gctggagatg gagatcagcc acatcaccag
    17821 aaaaaaggcc atcatcgcgg tcataaagtc ggcataagca atcttccacg atccatgtgc
    17881 tgccccgtgg cttttggctt tgcgtcgttt gacgacaata atcggatgcg cttgattctt
    17941 catgcttcct cggttgtcgt ctgttgttgc ggatttttca ccgcacgcac atgctcttcc
    18001 agttcaataa acgacggacg ttcgctggaa tagagcgttt tgcgaccaaa ctcaacggcg
    18061 ataggcggtg cgtaaccgtt cagattagaa agcagtgtga ctttgacgca ctgcatcatt
    18121 ttgctggttt cggcgctttt ctgacgtaaa acactcgcta atggggaaat aaatccgtaa
    18181 gccaataaaa tgccgaggaa agtccccacc atcgcatgtg cgataagcgc ccccagctcg
    18241 gcggcaggac gatcggctga acctaacgcg tgaacgaccc ccattacagc cgcgacaata
    18301 ccaaacgccg gaagtgagtc cccgaccagc gccagactgt tcgccgggac ttctgcctcg
    18361 ctttcgtgcg tctcaatctc ttcatccatc agggcttcga tttcgaaggt gttcatgtga
    18421 ccgctgataa tcaggcgcag ataatcgacg ataaaatcaa gcatgacgct atccgcgagg
    18481 atgcgtgggt agctggcgaa gatctcgctc tcacggggat tttcaatatc acgttccagc
    18541 gaaaacatcc ccatctgccg cgatttcgcc atcaaccgat aaagcagagc cagcagatcc
    18601 atatacattg ctttggtgta tttggagcga cgaaacagca acggcagcgc cttcagcgtg
    18661 cctttaatcg ctttgccatt attgccgacg ataaacgacc caatccctgc accggcaata
    18721 atcaccagtt cagcgggttg atagagtgct ccaaggcttc caccggtcat caaataaccg
    18781 ccgaaaactg taccgagaac aaccaggtaa cctaataaga taagcacgac atcatccttc
    18841 cactgttgac catgacagga tgttcagtcg tcaggcgtta acgcgcgatt ggggcaaaaa
    18901 aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc cacatcgtat cagttaaaca
    18961 gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt ctgggaaagt
    19021 ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa gctgccaaca
    19081 ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga aagttgcagt
    19141 aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag cagcggtcct
    19201 tcttctgctt gcgggcactg ttcaaggtat aaacggtagg ctttgatcac cgcatcgacg
    19261 ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat cgaagcatga
    19321 acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc tttcggcggt
    19381 gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa ctgtgtttcg
    19441 ctttccagca tctgcaaacg agcgcccagg gtgatcaatt ccattgccag ctgaatatcc
    19501 cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg cgcagcgctt
    19561 cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg gtatgaattt
    19621 gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg tggctgtcaa
    19681 aacggaagtg acaaaccagc tgattggttt ctgccagctt aaccatttgc ggaagagtca
    19741 gtgccgctaa tgttgtcgcc atttcttcat ttatgccgag acgaaacata gcggacgctt
    19801 tgtcctgaac aatcagacgc tgtgcaagta gtaaatatga caagttgatg tcataaatgt
    19861 gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt tatttttatg
    19921 cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa ttagcattag
    19981 aatagttgcg ataagctgca ataagcagag ccaccttttt ggtttaataa gtccttacaa
    20041 atagaagggg gtctttacac ttatctaaga tttttcctaa atcgacgcaa ctgtactcgt
    20101 cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg atgcagatca
    20161 cacaaaacac tc
//
LOCUS       DAZUCE010000076        20044 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZUCE010000076 DAZUCE010000000
VERSION     DAZUCE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.076"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            117..635
                     /gene="yfgH"
                     /locus_tag="ACHY03_002322"
     CDS             117..635
                     /gene="yfgH"
                     /locus_tag="ACHY03_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFS3905543.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            651..1190
                     /gene="yfgI"
                     /locus_tag="ACHY03_002323"
     CDS             651..1190
                     /gene="yfgI"
                     /locus_tag="ACHY03_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFS3905544.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1283..2860)
                     /gene="guaA"
                     /locus_tag="ACHY03_002324"
     CDS             complement(1283..2860)
                     /gene="guaA"
                     /locus_tag="ACHY03_002324"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFS3905545.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2929..4395)
                     /gene="guaB"
                     /locus_tag="ACHY03_002325"
     CDS             complement(2929..4395)
                     /gene="guaB"
                     /locus_tag="ACHY03_002325"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFS3905546.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4557..5927
                     /gene="xseA"
                     /locus_tag="ACHY03_002326"
     CDS             4557..5927
                     /gene="xseA"
                     /locus_tag="ACHY03_002326"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFS3905547.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5924..6139)
                     /gene="yfgJ"
                     /locus_tag="ACHY03_002327"
     CDS             complement(5924..6139)
                     /gene="yfgJ"
                     /locus_tag="ACHY03_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFS3905548.1"
                     /translation="MELHCPQCQHVLDQDNGHAYCPSCGKVIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6208..7680)
                     /gene="der"
                     /locus_tag="ACHY03_002328"
     CDS             complement(6208..7680)
                     /gene="der"
                     /locus_tag="ACHY03_002328"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFS3905549.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLNVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7798..8976)
                     /gene="bamB"
                     /locus_tag="ACHY03_002329"
     CDS             complement(7798..8976)
                     /gene="bamB"
                     /locus_tag="ACHY03_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFS3905550.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8987..9607)
                     /gene="yfgM"
                     /locus_tag="ACHY03_002330"
     CDS             complement(8987..9607)
                     /gene="yfgM"
                     /locus_tag="ACHY03_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFS3905551.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9625..10899)
                     /gene="hisS"
                     /locus_tag="ACHY03_002331"
     CDS             complement(9625..10899)
                     /gene="hisS"
                     /locus_tag="ACHY03_002331"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFS3905552.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(11010..12128)
                     /gene="ispG"
                     /locus_tag="ACHY03_002332"
                     /gene_synonym="gcpE"
     CDS             complement(11010..12128)
                     /gene="ispG"
                     /locus_tag="ACHY03_002332"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFS3905553.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12155..13168)
                     /gene="rodZ"
                     /locus_tag="ACHY03_002333"
     CDS             complement(12155..13168)
                     /gene="rodZ"
                     /locus_tag="ACHY03_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFS3905554.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13453..14607)
                     /gene="rlmN"
                     /locus_tag="ACHY03_002334"
     CDS             complement(13453..14607)
                     /gene="rlmN"
                     /locus_tag="ACHY03_002334"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFS3905555.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14757..15188)
                     /gene="ndk"
                     /locus_tag="ACHY03_002335"
     CDS             complement(14757..15188)
                     /gene="ndk"
                     /locus_tag="ACHY03_002335"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFS3905556.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15329..16183)
                     /locus_tag="ACHY03_002336"
     CDS             complement(15329..16183)
                     /locus_tag="ACHY03_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFS3905557.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIECAVCQFICPQEAIRGVNSPLRYFRNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNERLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRAP"
     gene            complement(16183..17004)
                     /locus_tag="ACHY03_002337"
     CDS             complement(16183..17004)
                     /locus_tag="ACHY03_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HFS3905558.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNARHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLILAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16997..17626)
                     /locus_tag="ACHY03_002338"
     CDS             complement(16997..17626)
                     /locus_tag="ACHY03_002338"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFS3905559.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17623..20004)
                     /locus_tag="ACHY03_002339"
     CDS             complement(17623..20004)
                     /locus_tag="ACHY03_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFS3905560.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSM
                     DTLLDTKLVILWGHNPTETIFGHTNYFFQKMKQNGTRFIVVDPRYSDTVSSLADQWIP
                     LLPTTDNALMDAMMYVIISENLHDKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAK
                     DGIHKTPEWAEKITHVPAQSIRQLARDYATTKPAALIQGWGPQRHICGERTARGSTLL
                     ASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQ
                     DGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSA
                     KYADVLLPETSFMERWNIGETWGTASYLILSEKLIEPDFERRTDYDWLRDVAKKLGVE
                     AEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDP
                     QNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGK
                     NRANSTQYANPWLQEVQTQKLWLNPQDAKQRGIVEGDSVKIYNDRGVSIIPVEITPRI
                     IPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
BASE COUNT         4957 a         5435 c         4863 g         4789 t
ORIGIN      
        1 tattccctgc gtgaatttta ataaatttaa tctatccctt tatacgcaat acatttactt
       61 tcctcttttg atgatcttaa atgtcttatt tttcgtaatg tgtataacaa ggaatagtga
      121 tgaaatttaa aaaatgtctt ctgcctgtgg caatgttagc gtcattcact cttgcaggat
      181 gccagtcaaa tgctgacgat catgccgccg atgtttatca aaccgatcaa ctgaatacca
      241 aacaagaaac taaaaccgtt aatattattt ccattcttcc cgcaaaagtt gccgtagaca
      301 actcccaaaa taaacggaac gcacaagcct tcggcgcgct tattggcgca gtcgctggcg
      361 gtgttatcgg ccacaacgtc gggtctggca gcaattccgg aacgacggca ggtgcagttg
      421 gcggcggagc tgtaggcgcg gcagcgggtt ctatggtgaa tgataaaacc ttagtggaag
      481 gtgtttcttt aacctataag gaaggcacca aagtgtatac ctctacccag gtgggtaaag
      541 agtgccagtt tacgacaggt ttagccgttg ttattaccac aacgtataac gaaacgcgta
      601 ttcagccaaa caccaaatgt cctgaaaaga gctagtaatc aggaggagtc atgaagaaag
      661 tttttctttg cgccatcttg gcctccttaa gctatccggc tatcgcctca tcattgcagg
      721 atcaactctc tgctgtcgca gaagcggaac agcaaggtaa aaatgaagag caaaggcagc
      781 atgacgaatg ggtcgcggag cgcaacaggg aaatccagca agagaagcaa cgtcgcgcaa
      841 atgcccaggc tgccgctaac aaaagagcgg caacggcagc ggcaaataag aaagctcgtc
      901 aggataaact ggacgccgaa gccactgcgg acaaaaaacg cgatcaaagt tatgaagatg
      961 agctacgcag cttagagatt cagaaacaaa aactggcgct ggcaaaagaa gaagcccgcg
     1021 ttaagcgaga aaacgaattt atcgatcagg aactgaagca caaagctgcg caaaccgatg
     1081 tggtgcaatc tgaagctgac gccaacagaa atatgactga aggcggtcgc gatctgatga
     1141 aaagcgtggg caaagcagaa gagaacaaat cggacagctg gtttaattaa tcgatgttac
     1201 taacttcaag cctatgattc ttgaagataa aaaaccctct gtagtaacag agggttttgt
     1261 tcattcatag tgcagggtca aatcattccc actcaatggt agctggcggc ttgccgctga
     1321 tgtcatacac cacgcgggaa ataccgttca cttcattgat aatgcggttg gaaacgcgac
     1381 cgaggaaatc gtacggcaga tgcgcccagt gagcggtcat aaagtcgatg gtttcgaccg
     1441 cacgcagaga aacaacccag tcatacttac gaccatcgcc cattacgcca acggaacgta
     1501 ctggcaggaa gacggtgaac gcctggctga ctttgtcgta caggtccgct ttacgcagtt
     1561 cttcaatgaa gatggcgtca gcacggcgca gcaggtcaca gtactctttc ttcacttcac
     1621 ccagaacacg aacgccaagg cctggtcccg ggaacgggtg acggtacagc atgtcgtacg
     1681 gtagacccag ctccagacca atcttacgca cttcgtcttt gaacagctct ttcagcggtt
     1741 caaccaggcc catcttcatc tctttcggca ggccgcccac gttgtggtga gatttgatga
     1801 cgtgtgcttt accggttgca gacgccgcag attcgataac gtcagggtag atggtgccct
     1861 gcgccagcca cttcacgtct tccagtttca gcgcttcttc atcgaatact tcaacgaaaa
     1921 cgcgcccgat gattttacgt tttgcttccg gatcgttttc gccagccagc gctgacagga
     1981 agcgatcttc tgccggtacg tgaacaatgt tcagaccaaa gtgatcgcca aacatatcca
     2041 gaacctgctc tgcttcgttg agacgcagca ggccgttgtc gacgaatacg caagtcaggt
     2101 ttttaccgat agcgcggtgc agtagcattg cggttacgga ggaatccacg ccaccagaga
     2161 ggccgaggat gactttatcg tcgcctacct gctcgcggat gcgagctaca gcatcgtcga
     2221 taattttcgc tggcgtccac agggcttcac actggcagat atcacgcaca aaacgctcca
     2281 gcatgcgcat accctggcgg gtgtgagtca cttccgggtg gaactgtacg ccatagaagc
     2341 gtttttcttc gttagccata atggcaaacg ggcagctttc ggtgctggct acggtgacga
     2401 agtcggacgg gatagcggta actttatcgc cgtggctcat ccagacatcg agcagcggtt
     2461 taccgtctgc ggtcagcgca tcttcgatac cgcgaaccag tgcgctgtcg tttacgactt
     2521 caacctgcgc gtagccaaat tcacgttcgt tagaggcttc aacgtgaccg cccaactgca
     2581 ttgccatggt ctgcatgcca tagcaaacgc cgaataccgg tacgcctgct tcaaagacat
     2641 actgcggcgc acgcggactg ttttcttcag tagtactttc cgggccgccg gaaagaataa
     2701 tgccgcttgg attgaagtca cgaatttgtg cttctgtcac atcccacgcc cacagttcgc
     2761 agtaaacacc cagctcacgc acgcggcgcg caaccagttg agtgtactga gaaccgaagt
     2821 ccagaatgag gatgcgatgc ttatgaatgt tttccgtcat tgacgcttat tccgaggcaa
     2881 gtgaaacaga taatataaat cgcccgacat gaagtcgggc gaagagaatc aggagcccag
     2941 acggtagttc ggggactctt tagtaatggt cacgtcgtga acgtggcttt cctgaatgcc
     3001 cgcaccgctg atacgtacaa actccgcttt agtacgcagt tcgtcgatag taccacagcc
     3061 ggtcagaccc atacaggagc gcaggccgcc catctgctgg tgaatgatct ctttcaggcg
     3121 acctttataa gctacgcgac cttcgatacc ttccggcacc agtttgtcgg cagcgttatc
     3181 gctctggaaa taacggtcag aggaaccttt ggacatcgcg cccagggaac ccataccacg
     3241 gtaagatttg taagaacggc cctggtagag ttcgatttca cccggagatt cttcagtacc
     3301 cgccagcatg gaacctacca tcaccgcgct tgcgccagcg gcgatagctt tggcgatgtc
     3361 gccggagaag cgaataccac catctgcgat aaccggaata ccggtgcctt ccagggcttc
     3421 aactgcgtca gcaacggcag tgatctgcgg aacacctacg ccagtaacga tacgagtcgt
     3481 acagatagaa ccagggccga taccgacttt aaccgcgctg caaccagctt ctgccagagc
     3541 gcgtgcacct gcagctgttg ccacgttgcc gccgatgatt tgcagatccg gatatttagc
     3601 acgggtttca cggatacgct gcaaaacgcc ttctgagtga ccgtgggagg agtcgatcag
     3661 cagaacgtca acgccagcgg caaccagcgc gtcaacacgc tcttcgttac ccgcacctgc
     3721 gccaactgca gcaccaacac gcagacggcc ttgctcgtct ttacaggcgt tcggtttacg
     3781 ttccgctttc tggaaatctt tcacggtgat catgccgatc aggtggaatt cgtcatcaac
     3841 caccagcgct ttttcaacgc gtttttcgtg catttttgcc agcaccactt cacgggcttc
     3901 accttcacgc acggtgacca gacgctcttt cggcgtcatg taaacgctaa ccggctggtt
     3961 caggtcggta acaaaacgca cgtcacgacc ggtgataata cccaccagtt cgttttcttc
     4021 ggtaacgacc ggatagcccg caaaaccgtt acgctcggtc agttctttca cttcgcgcag
     4081 cgtcgtggtt ggcagaacag tctgcggatc agtcaccaca ccagattcgt gttttttcac
     4141 acggcgaact tcttctgcct ggcgttcaat ggacatgttt ttgtggataa agccgatacc
     4201 gccttcctga gccagagcaa tagccaggcg cgcttccgtt acggtatcca ttgctgcgga
     4261 aagcataggg atattcagac gaatagtttt cgtcagctgg gtgctgaggt cagcagtatt
     4321 cggcagaacg gtagaatgag caggaacgag gagaacgtcg tcaaacgtca gagcttcttt
     4381 agcgatacgt agcatgggca atatctcgac cagagtggtt aataaatatt gccgcggcat
     4441 tatacagagc gtaaccgatt gcatctaccc ctttttgcaa aaaatgcttg ctatccccga
     4501 agggcgggtt actatcgact gaataacctg ctgatttaga atttgatctc gctcacatgt
     4561 taccttctca atcccctgca atttttaccg ttagtcgcct gaatcaaacg gttcgtctgc
     4621 tgcttgagca tgagatggga caggtttgga tcagcggcga aatctctaat ttcacacaac
     4681 cggcttccgg tcactggtac tttacactca aagacgacac cgcccaggta cgctgcgcga
     4741 tgttccgcaa cagcaaccgc cgggtgacct tccgcccaca gcatgggcaa caagttttag
     4801 ttcgcgccaa tattacgctc tacgagccgc gcggcgacta ccagataatc gttgagagta
     4861 tgcagccggc cggtgaaggg ctgctgcaac agaagtacga acagctcaaa gcgaagttgc
     4921 aggctgaagg attgttcgat ctgcaataca aaaaaccact tccctcccct gcgcattgcg
     4981 ttggtgtgat cacctcaaaa accggtgctg cgctacatga tattttgcat gtgttaaaac
     5041 gtcgcgatcc ttctctgccg gtgatcatct accccaccgc cgttcagggc gatgacgcgc
     5101 cggggcaaat tgttcgcgcc attgaactgg cgaatcagcg caatgagtgc gacgtgttga
     5161 tcgttgggcg cggcggcggt tcgctggaag atttatggag ttttaacgac gaacgcgtag
     5221 cgcgggcgat ttttgccagc cgcattccgg tcgtcagcgc cgtcgggcat gagacggatg
     5281 tgaccattgc cgattttgtt gccgatctgc gtgcaccaac accgtcggct gccgccgaag
     5341 tggtgagccg taatcagcaa gagttactgc gccaggtgca atcgacccgt caacggctgg
     5401 agatggcgat ggattattat ctcgccaacc gcacacgtcg ctttacgcaa attcatcacc
     5461 gattacagca acagcatccg cagctccggc tggcacgcca gcaaaccatg cttgaacgcc
     5521 tgcaaaaacg gatgagcttt gcgctggaaa atcaacttaa gcgtgccggg caacagcagc
     5581 agcgattaac acagcggctg aatcagcaaa atccacagcc gaagattcat cgcgcgcaaa
     5641 cgcgcattca gcaactggaa tatcgtttag cagaaaccct gcgcgcacag cttagcgcca
     5701 cgcgtgaacg tttcggtaat gcagtaacgc acctcgaagc cgtaagccca ctgtcaacgc
     5761 tggcgcgtgg atacagcgtt actactgcta ctgacggcaa tgtactgaaa aaagtgaagc
     5821 aagttaaagc gggtgaaatg ctaaccacac gtctggaaga cggctggata gaaagtgaag
     5881 taaaaaacat ccagccggta aaaaaatcgc gtaaaaaggt tcattaagcc agcacgaatt
     5941 caacgcgttt tttcgatatc aagccatgcc cgtgctgaca gaagtaatct accgcaccgc
     6001 aggcttttaa cacctgcaac ggttgatggc agtccgggca aagagctttc atttcaataa
     6061 ctttcccgca ggatgggcaa tacgcatggc cgttatcctg atcaagcaca tgctgacatt
     6121 gtggacaatg cagttccata tgaattcctc cggtagccac ccctctacat tcatagaggg
     6181 aatggcagat aaaatactta cggataatta tttatttttc ttgatgtgct tcatcagacg
     6241 cttacgttta cgcatctggg ttggcgtcag ggtgttacgc ttgttcgcat acgggttttc
     6301 cccttctttg aactgaatac gaatcggcga tcccattacg ttcagcgatt tgcggaagta
     6361 gttcatcaag taacgcttgt aggaatcagg caggtctttc acctgattac cgtgaatcac
     6421 cacaatcggc ggattataac caccggcgtg tgcatatttc agcttcacac gacgaccgcg
     6481 taccagcggc ggttggtgat cttcaacagc catcgtcatg atgcgcgtca gcatagaggt
     6541 ccccacacga cgggtggagc tgtcatacgc ttcacgtact gattcaaaca agttaccaac
     6601 accactgccg tgcaaggcag agataaagtg cacacgagca aaatcgataa agcccagacg
     6661 gaagtccagc gtttctttca cctgctcttt cacttcctgg ctcaggccat cccacttatt
     6721 caccacaatg acaagtgagc gcccactatt gagaataaag cccagcagcg agagatcctg
     6781 atcggaaata ccttcgcgcg catcaatcac caacatcacc acgttggcgt cttcaatggc
     6841 ctgcaacgtt ttgattacgg agaatttctc tacagcatcg gtgattttgc cgcgtttacg
     6901 tacgccagcg gtgtcaatga gcacatactc acgtccatcg cgttccattg gaatgtagat
     6961 actgtcacgc gtcgtaccag gcatgtcata aacaacaacg cgctcttcac caagaatacg
     7021 gttagtgagt gtagacttac ctacgttcgg acgacccaca atcgccagtt tgatcggcag
     7081 actttgcggg tcgaagtcgt cttcctcttc ttcttcgccg ttctcttccg cttcaaattg
     7141 cgcccagtat tcagcgtctt cgtcgacttc ctcttgcggt gcgagatctt ccatccacgg
     7201 cagcagcaca tgctccagca gacttaatac gccacgaccg tgagacgcgg cgatcgggta
     7261 gatttcacct aaaccaagcg agtagaaatc aaccactgcc tgatcgggat ccagaccgtc
     7321 agttttgttt gccaccagga aggtcggttt ttcacgggag cgcagatgtt tggcaatcgc
     7381 ttcatctgcc ggcatcaggc ccgcgcgcgc atccaccata aacagtacga cgtccgcttc
     7441 ttcaatcgcc agcagcgact gttccgccat gcgggtttct acaccgtctt ctgtgccatc
     7501 aatcccgccg gtatcaatac agataaactc acggccttca atttccgcac gaccgtactt
     7561 acggtcacga gtcagacccg ggaaatccgc aaccagcgca tctcgggtgc gagttagacg
     7621 gttaaataac gtggattttc ctacgttagg gcgcccgaca agcgcgacca caggtaccat
     7681 gtttaaagcc tcatttttat aaatcatcag acaacgcacg ctatattcgc gtcgttgtta
     7741 aaaacaggaa aacggcccct gtccaggagc cgttttcaaa gtgaacgaca gagacgatta
     7801 acgtgtaata gagtacacgg ttccgtcttt tgcctggatc agcagtttgc cgtcagcggc
     7861 aaccggttca gtctggaaac cggaactatc aactttttgc tgggcaacga aacgaccatc
     7921 ttcgacgtta atccagtgca aataaccttc actgtcaccg accactaggt tgccattata
     7981 cagcaccgga gaagtcagca ggcgatgcag cagatcgctt tgtgtccaca gcgtaacgcc
     8041 gccatcaatg gtcaacgcca tcacccggtc attttgatcg accagataga tgcgattgcc
     8101 gtcgacgatg aaatcattca ccgaacccag ttcgcgtttc cacataatct gaccactgcg
     8161 cagatcaagc gccgtcaggt taccattata ggccagcgcg aaaacaacgc cgttaacaac
     8221 gacgggagtc gtgtcaacat cgctcagacg gtcaatttcg gtagaaccgg tcgcctggga
     8281 aatacgctgc tgccaaatca tctggccctg ttccatcagc actgcgctga cgcgaccatt
     8341 atcgcccccc acgacggccg caccaaaagc cgttgccggc gcagactcgc cacgcaaaga
     8401 gagcgaaggc atatcgaggt taactgtcca tttgacagcg ccgtcagctt cgttcagcgc
     8461 ttgtaactga ccgttactgg tgtggattaa caccagaccg tcgctgacca ccgggcgcga
     8521 aagtgcttca cccgcgactt tagtttgcca tgccacagta ccatcgctgg tattcagcgc
     8581 gtaaacctgc gccttttcgc tgccaatgta gacatgccca ccagacacgg tcacaccgcc
     8641 agaaagtaat gcaggctctt tagagaacca gccatctttc tcggccaggc tgacagacca
     8701 gatttctttg ccatcatccg cattcagcgc ttttactaaa ccagcgcggt ccgctgcata
     8761 gacaacgttg tccgccagtg ccggatgaag attggaatag aagttgccaa tgccgctacc
     8821 aacggaagtg ctccacgccg tggtcggcgt aaactggttt tcaacggttg gcaatgggga
     8881 catctttacc acatcttctt cgctgttaaa cagcgaacag ccgcttaaaa gggtaacgga
     8941 aagcagtcct ggcagcagta atttacgcaa ttgcatcggg tccctctcag atggacaaat
     9001 tattaatttt catctgcatc atttcgctca gtgccggagt aacatcgctt ttcacgcctg
     9061 cttcccatgc actacgcgca ccttgcttat cacctttgct cagcaatgct tcaccacgca
     9121 ggtcggcaac aatggcagcc cacccttcac ctttgatggt atcaagggtt ttcagcgcgg
     9181 catcagcctg cttgagctgt acctgaacgc gagcaagacg cagatttatc acggctttga
     9241 gattttcatc gctcgtgtct gccagcccct gttgtaactg ggcggcagct ttctctagtt
     9301 catttttgtc aacaaattgc tgcgcaagtt ccaaagaagc cagcgcacca taagtatttt
     9361 tattttcagc agcaaatttt tccgccgccg ggatgctatc cggtttgcct tcgctcactg
     9421 cggtaaccgc attttgatag gcaagagaag cggagcgtgc agaatcaacc tgatggctgt
     9481 tccagtagcg ccagccaatc agtgcgccaa cgcccaaaat caccccaaca gccagtgctt
     9541 tgccattttc agcaaaaaag cgtttaaccg cttctacctg gtcgttttcg ttctcgtaaa
     9601 tttccacgct gtccttctcc ttccttaacc cagtaacgtg cgcaaatgcg cggctacgct
     9661 atcctgcgca actgccgttt gctcaccaga gcgcaaatcc ttcactactg ctgtgccgtt
     9721 agccacttca gactcaccca gcaccacagc aacgcgggca ccccatttat cagcacgggc
     9781 aaactgtttc ttaaagttgc cgccgccgtg gttggtcatc aatttcacgc ccggtaattc
     9841 atcacgcaga cgctcagcta atgccatagc cgcagattgt gtatcagcac ctgaagccac
     9901 caggtatata tcgacaacag gatcggcttt aaattccgga ttaacggcct gtactaacaa
     9961 tacaagacgt tcgaggccca tcgcaaaacc gacagccggt gttgcacggc cgcccagttg
    10021 ttccacaaga ccgtcataac gaccgcctgc acacacggtg ccctgggagc cgagactgtt
    10081 agtcacccac tcgaaaacgg tacggttgta gtaatccaga ccacgcacca gacgctggtt
    10141 tacggtgtaa gcgatccccg cgctctcaag cagtttgcac agaccggcaa aatgctcacg
    10201 agattcctcg tccagataat cacctaatgc cggagcgtcg ttgagaagcg cctgcacttc
    10261 cggatttttt gaatccagca cgcgcagcgg gttagtgtac atgcggcgtt tgcagtcttc
    10321 gtccagcttt tctttatgct gctcaaggaa tgccaccagc gcatcgcggt aattggcgcg
    10381 tgcttccagc gaaccgatag aattcagctc aagagttacg tgttcggaaa tacccagcgc
    10441 gcgccaccag cgggcggtga gcataatcag ttcagcgtcg atatccggac cttgcagacc
    10501 gaaaacttcg cagcccaact gatggaactg acgataacgc cctttctgcg gacgctcgtg
    10561 acggaacatc ggcccgatat accacagacg ctgttcctga ttgtacagaa gaccatgctc
    10621 gatgccggcg cgtacacagc ccgccgtccc ttcagggcgc agagtcaggc tgtcgccatt
    10681 gcgatcctca aaggtgtaca tctctttttc aaccacgtcg gtgacttcac caatcgcacg
    10741 tttgaatagc ggggtctgct ctacaatcgg caagcggatt tcactgtaac cgtagctgcc
    10801 gagcacgttt ttcagtgtgc cttcaatgcg ctgccagatg gccgtttcgc caggcaggta
    10861 atcgttcatg ccgcgaatgg cttgaatgtt ttttgccacg tttattctct ttctgaatat
    10921 aaaaatgaac cctcaacgct tccctcaatg tttcgggagc catgcgggtt caatcataca
    10981 cgggaagcga ggcgcttccc atcacgttat tatttttcaa cctgctgaac gtcaattcga
    11041 cgcgcttcgt ccagctgact ggctttcgca cgaatgcgtg cctccagctg gtcgatcata
    11101 tcgttgttgt ccagacggtc tttgcgcacg ccatcttcat agagaccgct tttcttgttg
    11161 ccgccggtga cgccgagtgt agaaaccagc gcctcacccg ggccattcac cacgcagcca
    11221 ataatcgaaa cgtccatcgg agtgatgata tcttccaggc gttgctccag cgcgttaact
    11281 gtaccgataa catcaaattc ctgacgcgaa caggtcggac aggcgatgaa gttgatacct
    11341 cgcgaacgga tacgaagcga tttcaaaata tcgaaaccga ctttgatctc ttcgaccgga
    11401 tcggccgcca gtgatacgcg cagcgtgtcg ccgatgcctt cagacagcag cagacctaaa
    11461 ccaatggcgg attttactgc cccgctgcgc gcgccacccg cttcggtgat ccccagatgc
    11521 agcggctgat cgatttgttt tgccagcaaa cgataagact caacagcgag gaagacgtca
    11581 gacgctttca cgctgacttt gaactgatcg aagttcaggc gatcgagatg atcaacatgg
    11641 cgcatggcgg attccagcaa cgcctgcggc gtcggttcgc catacttttc ttgcagatct
    11701 ttttccagcg atccggcgtt aacgccaata cggatcggaa tgtttttatc gcgcgcacag
    11761 tcaaccacca tgcgaatacg ctcttcatta ccgatattgc cagggttaat acgcagacaa
    11821 tcgacgccgt attccgctac tttcagcgca atgcgatagt cgaagtggat gtcagccacc
    11881 agcggcacgt taacctgctg tttgatgagt ttgaacgctt ctgccgcgtc catcgtcggt
    11941 acggagacac ggacgatatc agcgccaacg cgttccagcg ccttgatttg attgaccgtt
    12001 gcttcgacat ctgtcgtacg cgtattggtc atggactgta cggcgatggg agcaccatcg
    12061 ccaatcggca cattcccaac gtaaatacgt gttgattttc tacgttgaat tggagcctgg
    12121 ttatgcatga aaaatctccc gcgttacccg tctgttactg cgccggtgat tgttcggcat
    12181 tgagggtcag acgcgcaacc tggttagttc tgataaaacg actcagatcg acaggtttcc
    12241 cttgatactg gatctgtact gcggctggcg caccaatttt cagtttgtat ggtgcctggc
    12301 cggttaagtt caaattaccg tctttacgct gcataccgct aaacaatttt ttaccggtag
    12361 catcagtgac ctccagccag caatcggcag taaagttcat caccagcgca ttcggatcag
    12421 ccaccggcgt ggtcacgcca gcctgatcgg ttggcaacgg cgcagcacca tctggcgttg
    12481 ttgctgccgt tggtgccggg gtcgcggcgg tatcaacatt tgcctgcgaa ggcgaaacaa
    12541 ccgcattctg ttgcggatca acagctggtg ctggcgcagt tacggcaggt gtttgcgtgt
    12601 tggttgcggt agtatccaca gacgctggcg gcgtggacgt tgtagccggg tctgtagttg
    12661 tcgacgtatt taacggaaca ctctgcccct gctcgctatt actgctcagt tccgccgaag
    12721 attgatcggc catagtggtg atctcttcct gctgagcttt gtggtcttgc caccaccagg
    12781 caccgctcag gccgataacc acaaacaaca ccagccaggt gaaggtcatc agccagccgt
    12841 cgcgtttttt gcggcgttta ccgagggaaa aactctgcat cggcgcaact tttgcagccc
    12901 gtagtggagc ctgcttttcc agccctggca gcagttcttc ttctggaata tgtaccagac
    12961 gcgcataaga gcggatatat ccgcgcagga atgttgaagc aagatcggcg ggtgccttat
    13021 cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag tcgctcggca acggcctgct
    13081 gactaagtcc tagttgttcg cgagcattac gcaggcgagc gccggtagta agtgcttcat
    13141 tttggtcgtg cgtggcttca gtattcattc gctgcaggta cgtttaaatg agaattgaga
    13201 tgccggtgaa ccatcatgtt cacccacacc gcgaaacatc cgttaagtta accgttatca
    13261 tacagtataa gaccgtctgt gtgctcgtga caaagtccac attcactaag gctaacttac
    13321 tgttgcatcg ttacatactg ccttaaagtc agcaaaaacg caccgttaat attgaccaga
    13381 caattgcaac ttaatacttc attcataaaa cattacgcca cggtacataa agtaaccgtg
    13441 gcgtaatggc tatcagaccg ctttaatgtc gatggcttca ccctgcatcc gtttacgcag
    13501 ggtacgtttc gtacggtcga taacatcgcc cgccaactga ccacaggcag catcgatatc
    13561 atcaccacgc gttttacgca caatagtggt gaaaccgtag ctcatcagca cttttgagaa
    13621 acggtcgata cggctgttcg agctgcgtcc atacggtgcg cccgggaacg ggttccacgg
    13681 gatcaggttg atcttacacg gcgtatcttt cagcagttcc gccagttggt gcgcgtgttc
    13741 agtgccgtcg ttaacgtggt caagcatcac gtattcaata gtgactcggc cttgattggc
    13801 gttggatttc tccagataac ggcgcaccgc agcaaggaac gtttcgatat tgtacttttt
    13861 gttgatcggc acaatttcgt cacgaatttc gtcgttcggc gcgtgcaggg aaattgccag
    13921 cgcaacgtcg atcatatcgc ccagtttatc cagcgctgga actacaccgg aagtggaaag
    13981 cgtcacgcga cgtttagaca ggccaaaacc gaaatcatca agcatgattt ccatcgccgg
    14041 aacgacgttg ttcaggttga gcagcggctc gcccatgccc atcatcacta cgttagtgat
    14101 tgggcgctga ccggtgactt ttgctgcgcc gacgattttc gccgcacgcc aaacctggcc
    14161 gataatttcc gatacccgca ggttgcggtt aaagccctgc tgggcggtgg aacagaattt
    14221 acactccagc gcacacccca cctgcgaaga gacgcagagc gtggcacggt cgtcttccgg
    14281 gatatacacc gtttcgacgc gctgatcgcc tacggcgatc gcccatttaa tggtgccgtc
    14341 agatgaacgc tgttcttcaa ccacttccgg tgcgcggatc tccgccacct ctttcagttt
    14401 gccgcgcaac actttgttga tgtcggtcat ctcatcaaag ttgtcgcagc aatagtgata
    14461 catccacttc atcacctgat cggcgcggaa gggtttttca cctaaatctt taaaaaactc
    14521 ccgcatctgc tgacggttga gatccagcag gttgattttt ccatctttgg tggtgacgtt
    14581 ttcaggtgtg actaattgtt cagacatatg ctattccggc ctcgttatta cacgttatgg
    14641 cccctggagg gttgaaaaaa gaaacgcccc ggtgagcggc tgctcgtccg ggggcgctgc
    14701 attgtacaaa ttctggcgca cggatgccac gtttgcacgc ggcatttacg aaattattaa
    14761 cgggtgcgcg ggcacacttc gccttcgccg aagaaataag cgatttcgcg agcggcagat
    14821 tcgacggaat cagaaccgtg ggtaccgttt tcggtcaggc tgtcagcgta atcagcgcgc
    14881 agagtaccgg ccagtgcgtt tgccggattg gtcgcgccca gcagatcgcg gtgacgctga
    14941 acggcgtttt caccttccag cacggaaacc actatcgggc cagaggtcat gaattcaacc
    15001 agaccatcaa agaacggttt tccatcgtgt tcagcataaa agccacgtgc ctgttcaacg
    15061 gtcaggtgca gcattttggt gccgacaatt ttgaaccctg cagcttcaaa gcgcgcaaag
    15121 atattaccaa tgacgttttt tgctaccgcg ttcggtttga taatggaaaa agtacgttca
    15181 atagccattt ttacctctgt aaattgttct gttgttgtct gtaccagcgt acgagatggc
    15241 gcggattata atgagcaaca gggccgttga ctattgatga aggtaacatt tttttaaaat
    15301 aaaatgagtt ttagcaacaa ctcgactttt atggtgctct catcccatag cgatgacgtt
    15361 ggcaataaag acaattttgc gcattttgct gaaaagtaag aaaggatttt tgacaggttt
    15421 tacagacttt gcgattagca tgaagattca caatttttgc cggtagaatc gtcaattcaa
    15481 cggtcattgc ctgatgaaaa caaacagcaa tacaattttg gcatccgtta cacaaggcat
    15541 cattgatttg taaatggcct tcgttaagcg aaaagacctg ttgctcgcag gcccgccagc
    15601 atgcaccgca aagctgacac ttttgaggat ccatcatcgg gcgagcatca ctaatctggg
    15661 gccataaata atgtaaccga cgtaatcctg gttgtatttg tgtcatctga acatccgcac
    15721 gctgcacatg gaacaaacga cgccgggaaa cattcacctc actggtatgt ggagatacca
    15781 cagaccagcc ttcctcgcca tattgccgta agcgttcgtt taacagcaat aatgcgcgct
    15841 cccatgcctt cgcctgactc agatcaatcg cgatcgctgt gatcccctgc tctttatgcc
    15901 atagcaaaag ttcttccagc gtcggcgcaa tagtggtgaa aggtgcgacc agggtgtgat
    15961 tgcgaaaata acgtaaaggg gaattaacgc cacgaatagc ctcttgcgga caaataaact
    16021 ggcaaaccgc acactcaata cattgttccg cgtcgaccgc tatgccctct tctgtcagcg
    16081 ttagcgcctg tgccgggcaa gcctcgacac aggcatgaca tgttgcaaaa cgaaagcgtt
    16141 ggcgcgcgca ttccgcgcca atcgttatgt tatgacttaa cattagccga tactaaaaaa
    16201 gacaaaacgc agcataactt cactccccac tagcattacg ctaccgaata acgccgccgg
    16261 gatattcttt ttacaaccac cgacagcaaa gaacatcatc ccaatgacgg aaattcccca
    16321 gcttagtgtc agtatgggtt ttaactgtcc cagcatcgct aatggcgcat gtggcaatgt
    16381 ggctatctgc atcgcatttg ccgttaaatc ccctatccac aacggctgaa ccaacaaacg
    16441 caacagaacc aggagagcga tcaccaacac gcatccatta cgcagggcat caatctgcgg
    16501 caaaattccg ctaatacgaa acactgctac cagcgctgaa ccgagaattc ccactgtacc
    16561 taagaacata atcagcgtgt tgtaatgctg ccaggtgata atcgacgtat ggatgtaaac
    16621 ttgcgccatg cagaaaacat caacaatccc gaccactgcc gctaggataa gcaggagttg
    16681 ccagccgcct ttgcgtaaca tgaccagtaa tgtggataaa cccaatagcg ccagatacag
    16741 cgcaccaaag ataatttccc gactcagcca tgaactggaa aagtggctta gcgcatgcct
    16801 cgcattaagt ggataaccca gatgtaaggt agaaatcagt aaccctacgg cacctgccaa
    16861 aaaagccaac agtaatggcg gccgcataac cgggcgggcg ctggaggtga ttaaaatatg
    16921 acgatgaaga aacgccagcc acaacgtcat accgaccgat gcctgtaaca acagcgtaaa
    16981 agccattaac ggtaattcat gcatgatgtg acgcctcctt ttccgcgccc tgatgcggtt
    17041 taatcaccag atttggctgc gtaatcgacg aatcaggtaa acctttcaca tcactgacat
    17101 cgccatactt ttcacgcaac tcatcaatag ggccgaactt aatcgccccc aacgggcacg
    17161 ttgccacgca aatgggttgt tctcctttag cttgcagatc aatacagaaa tcgcatttcg
    17221 acatctgccc ggtctgctca ttcatttgtg gtgcgccgta aggacaggac caggcgcagt
    17281 aaccgcaacc gacgcactta tcagtattca cgcgcacaat accgtctccg ggacgcttat
    17341 gcatcgccat cgtcgggcaa tttttggtac agatcggatc cgcgcaatgg ttacaggaaa
    17401 cagaaagggt ataagcaaag acattatgac tcacaccgcc ctctccagtg gcgatgaagc
    17461 cacctccgtt cacttcatac acgcggcgaa aacgtcgccc aacctcaagg ttgtttttgt
    17521 ctttacaggc cacctgacac gctttacagc ccgaacaacg cgaagagtca ataaagaacc
    17581 ctaattgttt gtcgctgaca gatgcaaatt ctttaaattg gctcatgctt tctcaacctc
    17641 caccagcatt gtttgatgcg aattcccttt cgccagcgcc gtaatgcggg tcgaactcag
    17701 tacattggca cagcctccgc gatcgatacc ttgtgcatcc ggttgccacc atgcccccgc
    17761 ctgcatagca actacgccag ggatgatacg gggggttatt tccaccggga taatgctcac
    17821 accacgatcg ttataaattt ttacgctatc tccttcgaca atcccgcgct gttttgcatc
    17881 ttgcggattc agccagagtt tttgcgtctg aacttcttgc agccacgggt tagcatactg
    17941 ggtggaatta gcacggtttt tccctttcca ggtgatgagc tgcagcgggt atttcgccgt
    18001 cagtttatct tctggccctt caaaggcagg gacatagtgc gaaagcgcgg gaatttctgg
    18061 atcttgcata tcaaaaagac gcttagagaa gatctcaatt ttgccggaag gcgtcgggaa
    18121 aggattattc tgcggatcgc gaatatttgc ttcgaaagcg atgtgcggcg cacttttaaa
    18181 aagatgtcga cgtgtttttt gcagggtcgc gaagtccggc aaattttcat ccggcatcgc
    18241 cagacgagtt tgctcccaga tatgctcgat ccattgcttc tcatcacgtc cttgactgaa
    18301 ctcggcttcg acacccagtt ttttcgccac gtcacgcaac cagtcataat cggtgcgacg
    18361 ttcaaaatcc ggctcaatca gtttttctga cagaataaga tagctcgcag ttccccatgt
    18421 ttcgccgata ttccagcgtt ccataaagct ggtttccggc agcaacacat cggcatattt
    18481 cgcgctcgga gtcatgaaca gatcgctgag tacgatgaat tctattttag attcgtcttc
    18541 aaggagcttc gcggcctggt ggacgtccgg gttctggttc gccagataat tacctgccag
    18601 tgagaacagc aggcggatat tactgtcgag tttatcgaca cctttaagcc catcttgcgg
    18661 cgtaacttta gaagcatcat ccgccgcctg catccagttc ataatcgaga ttttcgcttg
    18721 taccggattt tctggcatat ctggtccaac gcagaattta cgattagaac taccgccgta
    18781 acctgccgcc cagccacctt tgatgccgac gttaccggta atcgaggcca gtaaagttga
    18841 acctcgggcg gtgcgttcgc cgcaaatatg tcgctgtggc ccccatccct gaatcaacgc
    18901 tgcgggtttt gtagtggcgt aatcgcgtgc cagttgacga atggattgtg ctggaacgtg
    18961 ggtaattttt tctgcccatt ccggcgtttt gtgtatccca tcttttgcgc caaaaaggta
    19021 ggcaaccaac gattcgttgg ctggtacgcc ttctggcata gagttttcat caaaaccgag
    19081 agtatatgtg tcgataaatg tcttatcgtg gagattctcg ctgataatga cgtacatcat
    19141 cgcgtccatc aacgcattgt ccgtggtcgg caacagcggg atccactgat cggcaagtga
    19201 agatacggtg tcggaatagc gcggatccac cacgataaaa cgtgtaccgt tttgcttcat
    19261 tttttgaaaa aagtaattgg tgtgaccgaa gatggtttcc gttgggttat gcccccagag
    19321 gatcaccagt ttggtatcca gaagagtatc catcgagtta ccgctggccg ccacgccata
    19381 cgtgtaaggc gttgccgccg cagtattacc cagactaact gagtgataat attcaagata
    19441 accaccggtt aaatttaaca gccgacgcgc catcgcatcg ccagaaaatg ttccaccaga
    19501 aacagccgtc ccgacatgga cataacgtga agccgggcca tattgttgcg tgatcctttt
    19561 caggttatcg gcaattaagg tcgtcgcctc atcccatgag atacgctcaa atttaccctc
    19621 gcctcgttta cctacccgct tcatcggata tttcaggcgg tctggatgat aaacaaattt
    19681 acgatagccg cgaccacgaa cacaggcgcg cataatcggc atttgtggat ccagttcgtt
    19741 atcgggaagc gtggtaattt gcgtcaccac gccgtcgcgc atatgcgccc gaatatcgca
    19801 cttaccaccg cagtcaaatg tgctacaggt gggcacgact ttatcagcta ctggagctgt
    19861 aacttctggc gacgcatctg gggattgcgc ctttgcggta gaagaaataa aaggcaaagt
    19921 gataagcgcg gaagtcgctt gtaaaaaact acgtcgtgat acttgcggaa atgtaatgcg
    19981 ttccttttcg tctggcgtat ccatcttgcg ttcccgaaaa tgaaaaaata aatattcacg
    20041 tatt
//
LOCUS       DAZUCE010000077        19849 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZUCE010000077 DAZUCE010000000
VERSION     DAZUCE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.077"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            139..1917
                     /gene="ygeV"
                     /locus_tag="ACHY03_002340"
     CDS             139..1917
                     /gene="ygeV"
                     /locus_tag="ACHY03_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS3905561.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGIVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1957..2436)
                     /gene="xdhC"
                     /locus_tag="ACHY03_002341"
     CDS             complement(1957..2436)
                     /gene="xdhC"
                     /locus_tag="ACHY03_002341"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFS3905562.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2433..3311)
                     /gene="xdhB"
                     /locus_tag="ACHY03_002342"
     CDS             complement(2433..3311)
                     /gene="xdhB"
                     /locus_tag="ACHY03_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFS3905563.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRSVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3322..5619)
                     /gene="xdhA"
                     /locus_tag="ACHY03_002343"
     CDS             complement(3322..5619)
                     /gene="xdhA"
                     /locus_tag="ACHY03_002343"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFS3905564.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAVVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6035..6790
                     /gene="actS"
                     /locus_tag="ACHY03_002344"
     CDS             6035..6790
                     /gene="actS"
                     /locus_tag="ACHY03_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFS3905565.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6869..6942
                     /locus_tag="ACHY03_002345"
     tRNA            6869..6942
                     /locus_tag="ACHY03_002345"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6901..6903,aa:Gly,seq:ccc)
     gene            7107..8321
                     /locus_tag="ACHY03_002346"
     CDS             7107..8321
                     /locus_tag="ACHY03_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3905566.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARTARDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEA
                     IAREWYEKRLDRWSPSYAEEMIETFEKDVFPYIGGRPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGVCA"
     gene            8865..9068
                     /locus_tag="ACHY03_002347"
     CDS             8865..9068
                     /locus_tag="ACHY03_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3905567.1"
                     /translation="MANNNSFIRLSEVMRRTGYGRAWIYRLISLGRFPKPVKIGSRSV
                     AFIESEVDEWINQRIDASRNNAA"
     gene            complement(9648..9884)
                     /locus_tag="ACHY03_002348"
     CDS             complement(9648..9884)
                     /locus_tag="ACHY03_002348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905568.1"
                     /translation="MNKKYQSITDIFAEQRRQLLHRILERQLELKRAKEISEPEELTP
                     LPGVKKHPQKRFALEDFVEKYPRRLKATKNRPPG"
     gene            complement(9942..10121)
                     /locus_tag="ACHY03_002349"
     CDS             complement(9942..10121)
                     /locus_tag="ACHY03_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS3905569.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKESE"
     gene            10318..10518
                     /locus_tag="ACHY03_002350"
     CDS             10318..10518
                     /locus_tag="ACHY03_002350"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFS3905570.1"
                     /translation="MRNHITHPQGRDSHNLNKFTWRFMQCLTDSIAIFTVTAATEHEA
                     RAQLPHAHLIFVARIRQEVTHA"
     gene            10511..10696
                     /locus_tag="ACHY03_002351"
     CDS             10511..10696
                     /locus_tag="ACHY03_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905571.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVHEIKGVQ"
     gene            10696..10899
                     /locus_tag="ACHY03_002352"
     CDS             10696..10899
                     /locus_tag="ACHY03_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905572.1"
                     /translation="MQEMTLAEASLKAQQIEVISRMIERMIERNHEEIDDHDLSVIAG
                     LIKQLSSNVAVWLMTEEEKRGEQ"
     gene            10957..11252
                     /locus_tag="ACHY03_002353"
                     /pseudo
     CDS             10957..11252
                     /locus_tag="ACHY03_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            11249..13387
                     /locus_tag="ACHY03_002354"
     CDS             11249..13387
                     /locus_tag="ACHY03_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFS3905573.1"
                     /translation="MKKAPNLKLQPKDKMTEVIIFAGSDAWAHAKEWSEWAGKHIAAD
                     DTPPVILGPKQLASLEDTKIIDKGRNYVRVYRAGEISETALLQIATLLAVAGVKEARC
                     YSGFVDQQPEDWTPRMAGLKDDAERGNSLVINLPAKTNFTGNYDDELKPRVECRPDGV
                     YWVTPKVDKQSGEIIRPETWLCASVELLGAGVIGNEHYRVMRWTDSTTNRLVTMAVPC
                     CGIGDSDGWRLLKANGLKVTTNGKHRIPLADWMQLGGQHEEWHLSTKAGWHFGAYIMP
                     DGTVIGESDKPVLFTGKSAAINGYSVAGTADSWRDSVARLAGGNPFMMLGIATSLSAP
                     LVGLVGADGFGVHFFENSTAGKTTTQSVASSLWGDPEAQRLTWYGTALGIANEAEAHN
                     HGLLPLDEIGQAASARDVYVSAYTLFNGFGKLQGAKDGGNRELKNWRAVAISTGEVDI
                     ETFLKTEGIKVKVGQLVRLLNVPMEKATKFHEYSNGKEHADALKEAWKENHGAAGREW
                     VKWLSGHQQEAKDTVRECRERWRNLIPESYGEQVHRVGERFAILEAALVLSGHVTGWA
                     VQECRDAILHNFNAWVKVFGTGNKEHKQIIEQAEAFLAAYGMSRFAPVNYDPASLPIP
                     ELYGYRESDGRYDEPVLFYVLPDPFGSHVANGFNKDAAAKVLHEAGMLKRPSSGRGWQ
                     IRTPRLKHLKGARLRVYGLLLAQDHDTESD"
     gene            13748..14077
                     /locus_tag="ACHY03_002355"
     CDS             13748..14077
                     /locus_tag="ACHY03_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905574.1"
                     /translation="MSEITVKNISPAVAGWWAKFRDDDGTEWYSPIAAWALCEVDYFG
                     AGNTRREILPVLTSELGMGPHPSDEGYCACLYLPDEKFTLSGEQGSFAWYPVNSNSNN
                     GNSGAES"
     gene            14074..14493
                     /locus_tag="ACHY03_002356"
     CDS             14074..14493
                     /locus_tag="ACHY03_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905575.1"
                     /translation="MTAKHTKNHNSVVKFVSARGRPTLLVPGKHLHYCDENHIPIVIV
                     WKRTVYADVTWLYDSLALMHRDLFEREEFRLNIEERAEKIYEQYAANSKRAARAITHH
                     FMTLYDLKAEDAEKAACDLFDMTMGIIQEYRNRERRP"
     gene            14490..14939
                     /locus_tag="ACHY03_002357"
     CDS             14490..14939
                     /locus_tag="ACHY03_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS3905576.1"
                     /translation="MTAQIAAYGRLVDDPQEKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALDRAKRQTGQQGNQPPAGGDDPYGDDIPF"
     gene            15107..15403
                     /locus_tag="ACHY03_002358"
     CDS             15107..15403
                     /locus_tag="ACHY03_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFS3905577.1"
                     /translation="MKPLKTGVIRDVLKDAKERELPIGHGVLRGALISYIHTTRYLNA
                     VIAGGARYDLNGQPCGEVTEEDKSVASELLKRRLAQIKQQNSQQQTTGDDGDDS"
     gene            15393..15686
                     /locus_tag="ACHY03_002359"
     CDS             15393..15686
                     /locus_tag="ACHY03_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFS3905578.1"
                     /translation="MIADSVAQKLEERGLWRRAATRWTEVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFGDVKKAADRTLAEMGINPQDEWRNYSFSDAGDDLALP"
     gene            16512..16751
                     /locus_tag="ACHY03_002360"
     CDS             16512..16751
                     /locus_tag="ACHY03_002360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905579.1"
                     /translation="MRKKLRKKVRKSIIKPITKSITQTNRAKPDMTRLAGGKATGKSA
                     QKNAQNETQQPRKTKPIRDDRKSAQIYRIRVSNFA"
     gene            16820..16981
                     /locus_tag="ACHY03_002361"
     CDS             16820..16981
                     /locus_tag="ACHY03_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905580.1"
                     /translation="MTERSPTEIIHGIIEEQEERELILMIEEQRERQRKQEATQPDSV
                     SAENQADVT"
     gene            17479..17871
                     /locus_tag="ACHY03_002362"
     CDS             17479..17871
                     /locus_tag="ACHY03_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFS3905581.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIIPAKITSRSQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(18703..19077)
                     /locus_tag="ACHY03_002363"
     CDS             complement(18703..19077)
                     /locus_tag="ACHY03_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905582.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
BASE COUNT         5330 a         4640 c         5184 g         4695 t
ORIGIN      
        1 ttccataatt tcattagaaa gtgataaaaa tcttatgttt ttatcaaagc caatttcgcc
       61 tgagttaata tcatcatgat ctgtgaacca tcaacgtctt caatgatcga cttttaagta
      121 agtctggaag gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa
      181 ccgacaattc agcgttttgc cagaatgctt gccagcgttt tgcagcttga ggttgagatc
      241 gttgatgaaa acttgtgtcg cgtcgccgga actggcgcgt atgggaagtt tcttggtcgc
      301 cagttgagcg gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt
      361 gtgacacagt ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga aaattgccgc
      421 gaaaaagcat ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt
      481 ttgattgccg ttacccacga gcaacaagag catatcagtg ataatttacg cgaattttct
      541 gattatgttc gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca
      601 ggagataaca tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtatta
      661 gttgttgatg atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa aacacttggt
      721 attgtacaaa ataatattat tgggaaacct atccgtttca gaccattaac atttgagagt
      781 aattttaccc atggacatat gcagcatatt gtttcgtggg acgataaaag tgaattaatc
      841 attggccaat tgcataacat tcagggccga caattatttt taatggcgtt tcaccaatcg
      901 cataccagtt tttctgtagc aaatgcacct gatgaaccgc atattgaaca attggttggc
      961 gagtgccgtg ttatgcggca attaaaacga ctcattagcc gtattgcacc cagcccatcc
     1021 agcgttatgg tggttggtga aagcggcacg ggtaaagaag tcgtcgcccg tgcaatccat
     1081 aagttgagcg gaagacggaa taaacccttt attgctatca actgtgccgc gattccggag
     1141 cagcttctgg agagcgaact gttcggttat gttaaaggcg catttactgg cgcttctgcc
     1201 aacggtaaaa cagggttgat tcaggcggcg aatacgggca cgctgtttct cgatgaaata
     1261 ggtgatatgc cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc acgtgaaatt
     1321 ctgccgattg gtgccagtag cccaatacaa gtcgacattc gcatcatttc tgcaactaac
     1381 cagaatttgg cccagttcat tgccgaaggt aaattccgcg aagatctctt ctaccgactt
     1441 aatgttatcc cgataactct gccaccgctg cgtgaacgtc aggaagatat tgaactactg
     1501 gtgcattact ttttacatct gcatacccgt cgtctgggat cggtttatcc tggcattgct
     1561 cccgatgtcg tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc
     1621 aatttgatgg aatatctggt taacgttgtt ccttcaggtg aagttatcga cagcacgcta
     1681 ttgccgccaa atctgctgaa taatggcaca acggagcaaa gtgatgtaac agaggtcact
     1741 gaggcacacc tgtcactcga tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa
     1801 atgatccgcg aggcgctttc acgtcataac agcaagaagc aagttgctga tgaactgggc
     1861 atcggcattg ctacgctcta tcgcaagatt aagaaatatg agttgttaaa cacataacct
     1921 cagtgaatcc ggcctgaatt caggccggat atccttttac ttcgttttct cgcaatccag
     1981 aactgtattt acaatcatct gataccccgt gcagcgacaa agatttcccg ccagtccgcg
     2041 acgaatttcc gtaatggtta atggcttctc gcgtggtttc gccagcattg ccgtggtagc
     2101 cataatcagg ccaggcgtac aaaacccgca ctgcactgcg ccggatttcg cataagcctg
     2161 ctgaacatga gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga tctcttttcc
     2221 ttcagcccag gcggcaaggt ataagcaact gtctattgct gtgccgtcga ccagcaccgt
     2281 acaggcacca cattcaccca cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg
     2341 gagtaattcc gagagcggcg tgcctggtgc ggcatgaagc tgaaaaggca tcccgttaat
     2401 ggtgcattcg atggtaattg tttcgctgtg attcattgca attttccccc cgccgcggcg
     2461 acggcttcgc taatcacttt tttggtcatc gtctggatga gatgcagacg aaactcttta
     2521 ctggcccgcc atgaagaacg cggggcgaca tcttgcagga cagattcgct gatagcttca
     2581 agcgtttgca ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc
     2641 ggcgttggcg cggcaacacc aaatgccaga cgtaattcgc tgaaattgcc gttatccagt
     2701 cggcaatgtg cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat ggcatattta
     2761 aaatgcgcgc tgcccacgtg ttctttcggc tggggcggaa aatgaaaggc gacaaggatt
     2821 tcgtcatgct caagagacac tttgcccggc ccggtgtgaa agccattaat cgggacgaaa
     2881 cgaacactgc gtggggagtg gatctccagt ttcgcgtcat aaatgagcgt tggcgtggca
     2941 gaatctgcgc tggtggcacc gttgcaaata tttccaccgt aggtagcgac gttacggatc
     3001 tgcggtccag caatggacga ggccgcagca cataacgccg ggagatgacg ttgagttata
     3061 ggatcttcta ttagctgggt aaatgtcgtt gcagagccga tacgtagcga gccatcttcc
     3121 gccagcgtaa ttccccgcag ctccgccaga ttatgaatat caacaatatg gcgataacgg
     3181 tcattgtgat ggtggagctg aatcagtacg tcagtgccac cggcgagcag tttggcctgc
     3241 gggttgtcag ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca
     3301 aaatcaaaca tgatgttatc ctcaaatcaa tcctgccaga tgaaattctt catataaccg
     3361 ttttggcgtc agcggcagtg tattgattgc aacaccggta gccatcttca ccgcgttacg
     3421 aatagcagcg gcaacaggaa ttattggtgg ctcacccagt gacttatgtc cgtatgcgga
     3481 ttgcggctca ttgatttcga cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg
     3541 cattttgtaa tccagcagat tggggttacg gaccacgccg cttttcgcat cgatgatcat
     3601 ctcttcaaat agcgcccagc caatgcccat tcccattccg ccgtgtacct gaccttctgc
     3661 cagcagcgga tttagaatat gccccgaatc atgaacgttg aggatgcggt tgatggtgac
     3721 tttgcacagc gcaatatcga ccgtcagatc aacaaaggta cagccgaacg ccggtgggtt
     3781 agtggtggtt ttgatggagc tttcagcaga gagctgcccg ccgcgttcag ggtggtagaa
     3841 agcgtccatc gccaaatctt ttaacgacat taacggctct tccggtcgtt caaccagcac
     3901 gatatggcct tttatcaggg tcagattcat cgctgactga tgtagcatga ctacggcgtg
     3961 agcgatgatt ttctctttta ataacagtgc cgcactgcgc agcgcaggcg cagcaacata
     4021 gctctggcgt gaggcaaatg cgccgggatc gaacggcgta acgtcggtat cttgtgttga
     4081 aataacgcga acgtcgctga ccggaacccc cacggtttct gccaccattt gcgagaagac
     4141 ggtgtcggca ccctgaccga tttccgtcgc gccgctttgc acgttgatgg ttccatcctg
     4201 attcatcaga aggcgcgcgc ctgctatttc tacgccgaca ggccaggtat tagaggtgta
     4261 gctaaaacag gcgacgccaa cgccgcggcg taaattgcct tgctggttct ggcattctgc
     4321 acggcgtttt tcccattcaa agattttccg gcctttttca agacactccg gcaaccctgc
     4381 gctgtaaata cgtttgcccg tgagcggatt agcatctcct tcgcgtgcgg cgttgcgtaa
     4441 acgaatttca acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca tagactcaac
     4501 ggcaaatacg acttgtggcg cgccataacc acgcatcgca ccagccgagg ggaggttggt
     4561 atagcaggtc tttgaactgt aagcgtaggc acaacgagga taaaggtaag cgactttatt
     4621 tccccctgca gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt tagacagaac
     4681 atccagacta taacctttca atgttccgtc gcggttcacg cccatttgcc cgtcaatagt
     4741 aaaagcgtgg cgggtacggg ttgcgaggaa acactcttca cggctaaggg aaactttcac
     4801 cggaatgccg ccaagcttgc tggtcaggaa tgccgccatt ggctcttcca gtacatcctg
     4861 tttattacca aaaccgccac cgacaaatgg tttgatgact cgtacgcatg accagggaat
     4921 atccagcgcc tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc tggaaactat
     4981 ggtaattcgc gagtcatcct ccatccacgc cagcgatgtt acgctttcca tgtgacaatg
     5041 ttgaataacg ggggtctgat agtgcccctg tacctggtag tcggcggcat cgattgtttg
     5101 ttggacattg cccgtcgaca tcgtgctttg tttcagtaaa ttgccgccgt tatggattgg
     5161 tgcagcgtcc tctgccagcg ccgcttctgg cgtggtgata acgggtaatt cttgccactc
     5221 aatgctgacc aattgcgccg ctttttctgc cgtgagttca tcgcgggcca cgacgatggc
     5281 aacggcgtca ccatgatgac gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg
     5341 cttgttttcg tcaagtgtcc atgcatgccc agctgtagcg aatgggatat caggcacatc
     5401 ttcccaggta aaaatcgcca gtacgcctgg taaacttctg gcttgttcat cattaatact
     5461 tacggcataa ccatgtgcga tagggctacg tacatatttc gcataacaca tgcccgccat
     5521 aacataatcg tcagtatatc gtgcccgccc ggtgacctta gcaatggcat caacgcgcat
     5581 gcatgattca cccgtagcgg ttgcttcccg cgcttccata gaaatcccct cgatttgaac
     5641 atttggttct tatggcaaag atttagtcta agcaggcaag atttacgcca gaaacgacga
     5701 taagcgttag gggaggaagg aagtgtgatg acgttaaaaa aacacagata tgatacgaaa
     5761 caggactctg aataggggca gtgatacgta tcaatatgag tggcgataaa atcaatgtga
     5821 taaattggga ttattacgca tcaggatggg aaatagggta aaaaagtcag gaaattcgat
     5881 acctctacag cgaaaatttt gctgatgata cttccctttg ccaaatgaaa ttaacctctt
     5941 tctctattac cgcgtttttc cagaagcaag agattgggta tcaagaggct ggctgctatg
     6001 atatggcgtc ttgtttttta agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa
     6061 atctctgggt atcgtgatgt tgttatcggt tggactgctt ttggcgggct gttcgggtag
     6121 caaatcatcc gatacaggaa cgtattccgg ctccgtttac accgtgaaac ggggggatac
     6181 gctatatcgt atttcgcgca ccacgggaac cagcgtaaaa gagctggcgc gactgaacgg
     6241 catttccccc ccttacacca ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag
     6301 tagcagtagt acacgtaaat caaccgccaa atcaacgacc aaaaccgcat cggttacacc
     6361 gtcatcagcg gtaccgaaat cttcctggcc gccagtaggg caacgttgtt ggttatggcc
     6421 aacgacaggg aaagttatca tgccgtattc gacagcagat ggcggcaata aagggattga
     6481 tatctctgct ccacggggta cacctattta cgccgcgggt gcaggaaagg tggtgtatgt
     6541 gggcaaccag ctgcgtggct acggtaatct catcatgatt aaacacagtg aagattacat
     6601 tacggcttac gcccataatg acacgatgct ggtaaataat gggcaaagcg tgaaggctgg
     6661 gcaaaaaatc gccaccatgg ggagcacgga tgcggcatct gttcgcctgc atttccagat
     6721 tcgttaccgt gcaacggcaa ttgatccgct acgttacttg ccgccgcagg gcagcaagcc
     6781 aaaatgctga tggcgaatta atcagcagtc agcagcgcgg cacttgctaa ggagagcgta
     6841 aggtttataa tgccttacgc atctcgaagc gggcgtagtt caatggtaga acgagagctt
     6901 cccaagctct atacgagggt tcgattccct tcgcccgctc caaacaaaca tctaccaacg
     6961 tctactaaag tctaccaatc ccccgaatat caaggcctcc agcgattttc ctcgtctaca
     7021 accgtctaca gtcatctacc taaatctaca cacagaagtg tatagtaatg cgtataggac
     7081 aaaatcgttt tttgggggct atacacatgt tgctttcaga ccttgctatc cgtcgcgcga
     7141 agccaaaaga gaaagcctat acactcaatg acggcaacgg gctttcgtta ctcattgagc
     7201 cgaacggttc gaagggctgg cgattgcgat acaggtttgc aggcaagccc aaaatgttgt
     7261 cgctgggtgt ttatcctgag gtgtcccttt ctgatgctag gacggcaagg gatgaagcca
     7321 aaaggcttat cgctggtgga gttaatccga gtgaggtcag aaaggcccaa aagcgagaac
     7381 aggcgaacaa atgcggcaac acctttgagg cgatcgcccg tgagtggtat gaaaaacgcc
     7441 tggaccgatg gtctccctca tatgcagagg aaatgataga gacgtttgaa aaagacgttt
     7501 ttccgtacat tggaggtagg cccatcgcag agattaagcc gatggaattg ctcgcggttc
     7561 tatcccgtct taatgaccgt ggcgcaactg aaaaattgcg taaggttcgc cagcgatgtg
     7621 gcgaagtgtt ccggtacgca ataattaccg gacgcgctga atataacccc gctcctgatc
     7681 tggcaagcgc atttgcacca cacaaaaaag agcattacgc gtatctgaaa gttgatgagc
     7741 taccggaatt tttccgcgtt tttaatacgt acaccggaag ccagatcgtc aagctgggta
     7801 tgcgattgtt gattcttaca ggacttcgcc caggtgaatt acggcaggca gagtggcagg
     7861 agattgattt tgataatcgt ctgtgggaag tgccgaaaga acgaatgaaa atgcgccgtc
     7921 ctcattgcgt gccactttca gaacaggcga tcgctattct ggaacagtta aaacacatga
     7981 cggggcagta tccgtttatt ttccctggtc gaatacagca cagtaagccg atgagtgaaa
     8041 tggctatgaa cgtgcttatt cgtcgtattg gctatgcagg gcgcgttact ggtcatgggt
     8101 tccggcatac catgagtacg attttgcatg agcagggtta taactccgcg tggatagaaa
     8161 cgcagctggc gcacgtcgat aaaaactcga tccgtggcac atacaatcac gcgcaatacc
     8221 tggacggacg gcgggagatg ctccagtggt acgccgacta tatggactca ctggaacagg
     8281 gcggtaacgt ggtgcacggc aagtttggtg tatgtgcata aaaacgactg gacaagtata
     8341 cagatgtata ctaacgtaca cggaaagcta caggtaatat ccttacagat ggtttttcac
     8401 gcagttaccc catcatgtat ggacggcgct ccttgtaaca acgaagatgg agtcttctac
     8461 gcagctgaaa agctggatgc tctttaacag cctttggtgc tttcctgatg caggaaagaa
     8521 tagggataca tgcaccttta caatgcatgt aatgattcac caatgaggct gcggagcgct
     8581 gacaaagcgc aattgctctc ttgcctgccc accatgccct gaaaaagggc gctctttaaa
     8641 actactggac gtcacgccta atcgtgtgac cccgccgaaa agcgggaata gctctacctt
     8701 gctggagcat accaaccacc aaccaaacaa tctgaattta tcttatgcaa taccacaaca
     8761 tgttttcatg ttgtggtttt tgtcgtgttc gtaatttgac gcaaataaaa ctcttgctaa
     8821 gccacaggag gggattttcg cgtcaacatt aaggagattg ataaatggct aataacaatt
     8881 cattcatccg tctttctgag gttatgcgcc gtactggtta cggtcgcgca tggatttatc
     8941 gcctgatcag cctggggcgt ttccctaaac cggtaaaaat tggttcccgc tccgtggctt
     9001 ttattgaaag tgaagtcgac gaatggatta accagcgtat tgatgcttct cgcaacaacg
     9061 ccgcgtaata atcttttggg agggggatat tctcccccag cactaaacct gattcgatag
     9121 caggagtttt caatgaaacg tccgaatgtg cccgtacagg ggcagggctt cgttcagcct
     9181 gaaatcagcc agaacgatat attgattcgc cacaaagcag agtcgcgtat tgattcccgc
     9241 ctgtttgctg aacgcatcgg cataaagcac aaaagccttt attcgcttat tcagaagcac
     9301 aaaaccggat tgcgtgaact gggtatcctt ccgtttcaaa cggagcgatt aaaaaccaat
     9361 caacacggag agcgtgaaca taagtttgct ctgctgaata gcgatcagtt tgattacatg
     9421 cgccatatcg tgagccattt cgccgctgag cgtatgaaac acgttaagca atacttagag
     9481 gaggttttat caaattatcg cgctggtggc tctgtacggc gcgaatattt gcatgatgca
     9541 ggaatggcag gcaaaacaaa ggcgaccacg aaagggtcgc cagtggagat aaaggaaatc
     9601 aagcgcatca ccaactatgc catgagtgta gctggtggac aaggctatca gccaggcggt
     9661 cggtttttag tggcttttaa ccgccgtggg tatttctcga caaaatcttc aagggcaaat
     9721 cgtttttgtg ggtgtttttt cactccggga agaggcgtta actcttcggg ttctgatatc
     9781 tctttagcgc gtttcagttc cagttggcgc tcaagtatgc ggtgcaaaag ctgtcttcgt
     9841 tgttcggcaa aaatatctgt aatggattgg tactttttgt tcatgctgct gccctgtaaa
     9901 gcaatgcgcc gtagtacctc acaccacggc gctgatagtg attattctga ttctttggcc
     9961 ttgcggcgct ggcggcgttt gatctcgcct cgcatggctg taacgataaa ttgcgcagtg
    10021 ctctcgcctg tttcttttac ttgctccata gcctcaacaa cttcatgcgg ggcgcgagcc
    10081 tgtaatttct gtgatttgtt attgatatgg tctctttcca tttctgcttc cctttgtgat
    10141 tactggaatt cagtatacac gaaaaagacc aaaacaaaac gcttgaagtg gattccactt
    10201 agtgattata ctggaatcca gttgagtgtt tatcactcaa tgcaaacgag gctgaaaagg
    10261 attgccgtcc tttcgcagcc tctgaccacc aacgatagca agagtatcga ggcagctatg
    10321 agaaatcata tcacacaccc gcaagggcgg gactcgcaca acctgaataa attcacctgg
    10381 cgctttatgc agtgcctgac agacagcatt gcaatattca ccgttaccgc tgccactgag
    10441 cacgaagcac gcgcccagct gccgcacgca catcttattt ttgtcgcccg tatccgtcag
    10501 gaggtgactc atgcttaaaa ccttccgcgt cttttcccgt gctgttaaca agcgtggcta
    10561 taccgttggc atcacgcaga acgtgaaggc cgaaaacgtc caggctgcaa tcagcacagt
    10621 aattaacaaa tcagctaaag ccggtttatc cggcgtaatt atccacgccg ttcatgaaat
    10681 taagggggtg cagtaatgca ggaaatgaca ctggcagaag cctcactgaa agcgcaacag
    10741 atcgaagtaa tcagccgcat gattgaacgc atgattgaac gcaaccacga agaaatagac
    10801 gaccatgacc tttctgttat tgcgggactt atcaaacaac tttccagcaa tgtggcggtg
    10861 tggttaatga cggaggaaga aaaacgcggc gaacaataac ggctaaaaat aaacgggaaa
    10921 taaatatcaa gacgactcct tcaggaggct aattacatga aatcgaaaat atctggctta
    10981 actgccagta gcaaaactca ccccgaattt atgtgcgggg atatttatcg cgataagtac
    11041 ggcggcatcg tgacgattat cagcacgaga gaacgacgca tcacctaccg ccgtgaaggg
    11101 tatgactatg actgcgtgat gcccctgtat cagttcagtc gtgatttttc cctgatgcag
    11161 gccgtgccac gtggtaagcc agcaatacaa aagcagaggc gaacatccga aagatgaaaa
    11221 acatgattaa cgtattcagg agcaaaaaat gaaaaaagca ccgaatttaa agctccagcc
    11281 gaaagacaaa atgaccgaag tcattatctt tgcaggtagt gatgcatggg cacatgcgaa
    11341 agagtggagc gaatgggccg gaaagcatat tgcagcagat gatacaccgc ccgttattct
    11401 tggaccgaaa caactggcga gcctggaaga cacaaaaatc atcgacaaag ggcgcaatta
    11461 cgtgcgcgtt tatcgtgctg gtgaaatatc agagacagca cttttacaga tagcaacact
    11521 gcttgcggtg gcgggtgtaa aggaggcacg ttgctacagc ggctttgttg accagcagcc
    11581 ggaggactgg acaccgcgca tggctggact gaaggatgat gcggaacgcg gcaacagcct
    11641 ggtgatcaat cttccggcaa agacaaattt caccggaaat tatgatgacg aattaaaacc
    11701 ccgcgttgaa tgccgtcctg atggcgttta ttgggttacg ccaaaggttg ataagcaatc
    11761 aggcgaaatt atacggcctg aaacgtggtt atgtgcttcg gttgaattac tgggcgctgg
    11821 tgtcattggt aacgagcatt accgcgtgat gcgctggaca gattcgacaa caaatcggct
    11881 tgtcactatg gccgtgcctt gctgcggaat aggcgacagt gacggctggc ggttactgaa
    11941 agccaacggg cttaaagtca cgacaaacgg aaaacacagg atccccctgg ctgactggat
    12001 gcagttaggc ggacagcatg aggaatggca cttaagcacg aaagctggct ggcactttgg
    12061 cgcgtacatc atgccggacg gtacggttat cggcgaatct gacaagcccg ttttgtttac
    12121 cggaaaaagt gctgccatta acggttattc cgtggcgggt acggcggata gctggcgcga
    12181 cagcgtggcg cggctggctg gtggtaatcc gttcatgatg ctgggcatag cgacgtcatt
    12241 atccgcgcca ttagtcgggc tggtcggtgc tgatggcttt ggtgtgcatt ttttcgaaaa
    12301 ctccacagca ggtaagacca caacacaaag cgtggcatcg agtttatggg gtgatcctga
    12361 agcgcagcga ttaacctggt acggaacagc gttaggcatt gctaacgagg ctgaagcaca
    12421 caaccacggg cttttaccac tggatgagat aggacaggca gcaagcgccc gtgatgtgta
    12481 cgtatcggct tatacgctgt ttaacggctt cgggaaatta cagggggcaa aggacggcgg
    12541 caacagagag ctgaaaaact ggcgggccgt tgctatcagc accggagagg tggacattga
    12601 aacattcctc aaaaccgagg ggataaaggt aaaagtaggg cagcttgtcc gcctgcttaa
    12661 tgtaccgatg gagaaagcca cgaaatttca cgaatacagc aacggtaagg aacatgcgga
    12721 cgcgttaaaa gaagcctgga aggaaaacca cggggcggcg ggtcgtgagt gggttaaatg
    12781 gctgtcaggc caccagcagg aggcaaagga tacggtaagg gaatgccgcg aacggtggcg
    12841 caacctgata ccggagagct acggcgagca ggtccaccgc gtgggtgaac gtttcgccat
    12901 actggaggcc gcgcttgtgc tttccggtca tgtaactggc tgggccgtgc aggaatgccg
    12961 cgacgcgata ctgcacaact ttaatgcctg ggtgaaggtg ttcggtacgg ggaacaagga
    13021 gcacaagcag attattgaac aggctgaggc gtttcttgcg gcctatggga tgagcaggtt
    13081 tgcccctgtc aattatgacc ccgccagtct gccgattcct gaactgtacg gctacaggga
    13141 aagcgacggg cgttatgacg aacctgtttt gttctacgtg ctgccggatc cgttcggctc
    13201 acatgtggcg aatggattta acaaggatgc cgcggcaaaa gttcttcatg aagccggaat
    13261 gctgaaaaga ccatccagcg gtcgtgggtg gcaaatcagg acaccacggc taaaacacct
    13321 gaaaggggcg cgcctgagag tgtacggcct tttgctggcg caggaccacg acacggaaag
    13381 cgattaatct cgcgtatagg cgatgaaaaa atgttgtccc agttgtccca ctacgtatta
    13441 attgattgat tttactaaaa atacaataaa aaagtgggac aacattacag cattttggga
    13501 cgacatcggg gtattttggg acgacatagc gacattttgg gacgacacaa cgagcgttaa
    13561 ttaccctggc tggtgactgg gacaacataa aacccctcat gttgtcccaa aatcggctaa
    13621 tgttgtccca gttttcggag tgtattttta attaaatcaa tggattatgt gtaagtggga
    13681 caactgggac aactgggacg acgtgttttt gtatatatat aaaaaaataa attgagagga
    13741 tgaattcatg agtgaaatta ctgttaaaaa tatctcacct gcggtagctg gctggtgggc
    13801 gaagttcaga gacgacgacg gcacggaatg gtacagcccg attgctgcat gggcgctttg
    13861 tgaggttgat tatttcggtg cggggaacac ccgccgcgaa atcttacccg tgctgaccag
    13921 tgagcttggt atgggtccgc acccatccga tgaaggctat tgcgcgtgtc tgtatctgcc
    13981 tgatgaaaag ttcacgctct caggtgagca ggggtctttt gcctggtatc cggtgaacag
    14041 caacagtaat aacggcaatt cgggggctga atcatgacgg cgaagcacac aaaaaaccat
    14101 aattccgtgg tgaagttcgt atctgcccgt ggcaggccaa cgcttttagt gccaggaaaa
    14161 catctgcatt actgcgatga aaatcatatc ccgatcgtga ttgtctggaa gcgaacggta
    14221 tacgccgatg tgacatggct gtatgactcg ctggcgttga tgcaccgtga cctgtttgag
    14281 cgtgaagagt tccggctgaa tattgaggaa cgggcagaaa aaatctatga gcaatatgca
    14341 gcaaacagca aaagagccgc acgggcaatc actcaccatt tcatgacgct gtatgacctg
    14401 aaagcagaag atgcagaaaa agcggcatgt gatttattcg atatgacaat gggtattatc
    14461 caggagtacc ggaacaggga gcgcagacca tgacagcaca gattgcagct tacgggcggc
    14521 tggtggacga cccgcaggaa aaacagacca gcaagggcac accgatgacg ctggcgcgta
    14581 tggcggtatc tttgccatgc agtcaggcac aggacggaca ggcgacgtta tggctatcag
    14641 tcctggcatt tggtaagcag gccgacgcgc tggcgagaca tgaaaaaggc gaccttatca
    14701 gcgtggcggg taacatgcag atcagtcagt ggaccggaca gaacggagaa atacggcagg
    14761 gctggcaggt tatcgctgac agcgtaatca gtgcccgttc ggcgcgtcct ggcgggaagc
    14821 gggggcagca ggggaaggcg accgaagcac tggacagagc aaaacgacag accggacagc
    14881 agggaaatca gccaccagcg ggaggtgatg acccttacgg tgatgatatt ccgttctgag
    14941 cgacaggagc acaacatgaa ccagcagacc acacaacagg accagacagt acgtaaacca
    15001 tcacggcgca taccgtggca gcacatgaca aagcggcagc gaaaaaaacc acagacggat
    15061 taaccgcctg gctgaatact ggcccgagat tttcaacgtg gagaatatga agcccctgaa
    15121 aactggcgtg atccgtgatg tgcttaagga cgctaaggaa cgggaattgc cgattggtca
    15181 cggcgtattg cgcggcgcgt taatatcgta tatccacaca accagatacc tgaatgctgt
    15241 tatcgctggt ggtgctcggt atgacctgaa cgggcagcct tgcggcgaag tcacggaaga
    15301 agataaatca gtcgccagtg aactactgaa acggagactg gcgcaaatta agcagcagaa
    15361 cagccagcag caaacaaccg gagacgatgg tgatgatagc tgacagcgtg gcacagaaac
    15421 ttgaggagcg cggtttatgg cgacgtgcgg cgacccgatg gactgaggtt cttcttcatg
    15481 cagaaacgga cagggaacgc gaggaagctg ccagacgtcg ggcgatatgt atcattaaat
    15541 cccgtcgtat gcctgagcag ttcgtcacct ttggcgatgt gaaaaaggcc gctgacagaa
    15601 cgctggcaga gatggggatt aatccacagg atgaatggcg aaattacagc ttcagtgatg
    15661 caggcgacga tttagcgcta ccgtgatacg catctcattg ccgcatttgc tcaattatcg
    15721 cgattcgcta taatcaccac cgaaaagccg ttgatggtgt tctcctcggg caactggagc
    15781 ccgactgaat gaagccattc aacggttttt cggcactcat tccggtgtat ctgatcccac
    15841 acatcgggat gagtgaacga cgaaaacatc acggtgattt cgtcatgccg cgataagaat
    15901 gttccccttt gggcaactgg agccctctgt gcagaaggtt atcgcggtca ggttgtgata
    15961 aaaacgcgcc tctttgagca attgaagctc actgtaaagc ggcttatcac agcaggctgt
    16021 agcgggtgct catctctggg caactgaagc cctctgtaca gaggcccgtt acggctgacc
    16081 ctgaatgatt tcgccccgtg caattgaagc acccagcgga gttattcagg gttttttatt
    16141 gcccgtccac cagcaccaca tctgtagcat tgatgcgtat acattcgccg tcaattgcac
    16201 cattccggcc ctcaccgatc cgcagcgaga gcatatagcc agacggcggg aaatatgcct
    16261 tgcgaacttc aggatgctgt aaataagact gttgacgata tgggaatcag acgggatcgg
    16321 cgacattacg ggttatggaa ataacagcca ggtaaattgg tgaatatacg gcaggctgca
    16381 atcagagccg aacatagcgg cctccgtcac cattatcaga cagtagcggt agtccttcca
    16441 tcacagcaca atgctaccca cttcacagcc ccttggaagc ctcttcgatg gggtgaaaaa
    16501 tccctgaaaa aatgcgcaaa aaactgcgca aaaaagtgcg caaatcgatc ataaaaccga
    16561 tcacaaaatc gatcacacag acaaacagag caaagccaga tatgacgcgg cttgcagggg
    16621 gaaaggccac cggaaaaagt gcgcaaaaaa atgcgcaaaa cgaaacgcaa cagccgcgca
    16681 aaacgaaacc gatacgcgat gatcgaaaaa gcgcgcaaat ttaccggatt cgggtaagca
    16741 attttgcctg atttgggcaa aatacggtga cgccgtaaca gtgacattga caccaaaaca
    16801 gtgacacaca ggagggccga tgaccgaacg ttcaccaaca gagataattc acgggattat
    16861 tgaagagcag gaggaacgcg agttaatcct catgattgaa gagcagagag aacgccagcg
    16921 caaacaggag gccacgcagc cggacagcgt gagcgctgaa aatcaggctg atgtgacgtg
    16981 atggtgtcga actgagtagc tttaagcgac tctgtgactg gttttaaacg agtcgcaaaa
    17041 agagccggag aaaaaccacg aatctggggt tttttctaag cacgctgtac acggtggaac
    17101 atatggtggt caggagacgg caggagcaac gccagaacgc gcccccgtac cagtggcata
    17161 agccggagcg gttacggcgg taacttgttg attaaaaggc ggctcaattc tgagccgggt
    17221 agctgacggc gttaactggc tgatattgtt acaaagctca tttttgagct ttggcctaac
    17281 ctactgatat tttcggaaac ctcaatttga ggagaccgca aacgcaaaat tgcgctggct
    17341 acattcctca cttctgagga ctgttaacaa gctggctgaa agacagtcac aagctacgaa
    17401 attttcgtag tttaactttc atccagtgca tccctgaatt acgggtatgt aaccacagct
    17461 attacgaggt gattgttgat gcgtgatatt cagcaggttc tggaacgctg gggagcgtgg
    17521 gcggcaaata atcgcgagaa tgtgacctgg tcatccgttg ccgcaggatt taaggggatt
    17581 attccggcaa agataacatc acgctcacag tgctgtgacg atgatgcgat ggtgatatgc
    17641 gggtgcatag cccgtcttta ccggaacaat cgcgatctgc atgacttgct ggttgattat
    17701 tacgtgttgg gggagacgtt catggcgctg gcacggaaac atgggtgctc tgacacctgt
    17761 ataggtaaac gccttcacaa agcggagggg gttgttgaag gcatgttgat gatgctggat
    17821 gtgaagctgg agatggatcg gtatgttgag cgcgattcag tgacggcata acgcgcataa
    17881 aaaaaccgca gtcatgagga aactgcggtg gagaaatacg gcttgccaat caatcatgaa
    17941 aaacgtaatc gcctgataat tatgccacga gacaaaggac ggtgcaccct gaatggtcat
    18001 aatgaccact cagaccatcg aggcaaccat aacgaacatc gttacagttg gagcttttac
    18061 cccgttgggg gaaaaggtgc atacctgaat catcattatg gtgatcaagg gcatgtaaag
    18121 cccaccagcc tgattaaagg ttaaccggaa aaagccaggt atggagggag tgattagcca
    18181 cgaacaagcc ggacaccacc agcagaaggt attatgattt tcgtaatacc tttagcaaag
    18241 cgtgaatcat cattaccgta gggatggtca taatgaccac accttccccc accggaacaa
    18301 ggaaggctgg cttacgaaaa tttcgtaacc ctccccagca caatgtatta aataatcact
    18361 acaggccatt gattaaactt aattatcgcg aatcgcgaga attagccccg ccggaacaag
    18421 gaaagggaaa ttacgaaaat ttcgtaaaat ccccccacat ctcccacggt tgcccgttaa
    18481 gtgcaagcgc acaggcacga cctcattaac cagttaccag cgctcacgct caaacatcca
    18541 cagcaatggg gataaatcgc actctggcgc aatgttcact atcggggcga gcattcaccg
    18601 gaaaacgtca tacaggctta caggcggctt aaaattcgat gggcggagga ccggatggga
    18661 atcgggagac ctggacaaaa agtgcaggcc tccatgacac ggtcaggaca gtttgtgttt
    18721 aaccagaata tttagcgcct gtattggggc ttcgtggagc tcccgcagac attcagggtc
    18781 agtaacatgt tcctggtatc ttgttttctc aagtaacaag cggtgcatat gcagcatacg
    18841 ggcaagagcc gcgcacacct cctcaaatgc ggtatcccgc ttaccgtcac tgtcagagcc
    18901 acaaaacagc ggtacaacct ccacgccgtc gctgtgggcc tttagccagg catcaaccac
    18961 ggttaaatca tcggtgaaga accacgcact gttagcgctg aatcgcattt tccaggcttc
    19021 cggtactggt tgcggggagt atgaatatgc tggtggtatc ttgtcgtgag tatccatagg
    19081 ctatcgttcc gtcattctgt tattcaccac agcgctaaaa acgccatgaa ggagcagaat
    19141 accgattgcc tcgtttgctg tatgaggtgc ttaccacaca ttgggtacac agatgaatca
    19201 ggcggaagtg tgattaccgg aaaaaatcca tttcagtttg agggaaaaaa aaccgtagtg
    19261 cgataacact acggcatgcc accaaagaaa cacaacaaga ggtcaacaat gagtaatgga
    19321 catgaccaga cgattttatc atgccggaac aaaagttatg tgacacagtc ggataaaaac
    19381 aggtaaagcc caccagcttc ataggggata accggaaaaa ttccggtaaa tatttgcctg
    19441 agtaagtcat caagcgcctg ctaaatgtgt atagaaatgt gtataggatt gattttagaa
    19501 catgtggaaa tggctttatt actggtggca tggcttgttg ttctgttccc ttcgccctcc
    19561 aatttatctt cacctcatca acctgccatt tttgttgtat ctgattttct atattatctt
    19621 aattcaatcg cttcagactt ttcgcaatcg ccgtaacact tccccatttt tttgtaaata
    19681 aaaagcgttt acgcaatgca atccttttgt aacaacccag gtaaaatacc ttattttcaa
    19741 gttgttaaat aagtggttat ctacgctttt aacaaacgat tgatggaata ttttatgaaa
    19801 gggaaatctg cattaactct tctgctcgct ggtatatttt cgtgcggca
//
LOCUS       DAZUCE010000078        19833 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZUCE010000078 DAZUCE010000000
VERSION     DAZUCE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.078"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..262)
                     /locus_tag="ACHY03_002364"
     CDS             complement(<1..262)
                     /locus_tag="ACHY03_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFS3905583.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISIN"
     gene            complement(870..2234)
                     /gene="rhlE"
                     /locus_tag="ACHY03_002365"
     CDS             complement(870..2234)
                     /gene="rhlE"
                     /locus_tag="ACHY03_002365"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFS3905584.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQLQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKVLAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQLRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            2463..3134
                     /gene="cecR"
                     /locus_tag="ACHY03_002366"
     CDS             2463..3134
                     /gene="cecR"
                     /locus_tag="ACHY03_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFS3905585.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            3137..4132
                     /gene="hlyD"
                     /locus_tag="ACHY03_002367"
     CDS             3137..4132
                     /gene="hlyD"
                     /locus_tag="ACHY03_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFS3905586.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            4125..5861
                     /locus_tag="ACHY03_002368"
     CDS             4125..5861
                     /locus_tag="ACHY03_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS3905587.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5854..6987
                     /locus_tag="ACHY03_002369"
     CDS             5854..6987
                     /locus_tag="ACHY03_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3905588.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6998..8104
                     /locus_tag="ACHY03_002370"
     CDS             6998..8104
                     /locus_tag="ACHY03_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3905589.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(8066..8476)
                     /locus_tag="ACHY03_002371"
     CDS             complement(8066..8476)
                     /locus_tag="ACHY03_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFS3905590.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            8609..9370
                     /locus_tag="ACHY03_002372"
     CDS             8609..9370
                     /locus_tag="ACHY03_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS3905591.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTRKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            9367..10608
                     /gene="clsB"
                     /locus_tag="ACHY03_002373"
     CDS             9367..10608
                     /gene="clsB"
                     /locus_tag="ACHY03_002373"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFS3905592.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            10608..11564
                     /locus_tag="ACHY03_002374"
     CDS             10608..11564
                     /locus_tag="ACHY03_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFS3905593.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGENTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(11600..12313)
                     /locus_tag="ACHY03_002375"
                     /pseudo
     CDS             complement(11600..12313)
                     /locus_tag="ACHY03_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(12521..13225)
                     /locus_tag="ACHY03_002376"
     CDS             complement(12521..13225)
                     /locus_tag="ACHY03_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFS3905594.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDSSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(13361..13813)
                     /gene="moaE"
                     /locus_tag="ACHY03_002377"
     CDS             complement(13361..13813)
                     /gene="moaE"
                     /locus_tag="ACHY03_002377"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFS3905595.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRNSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13815..14060)
                     /gene="moaD"
                     /locus_tag="ACHY03_002378"
     CDS             complement(13815..14060)
                     /gene="moaD"
                     /locus_tag="ACHY03_002378"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFS3905596.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSNRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(14053..14538)
                     /gene="moaC"
                     /locus_tag="ACHY03_002379"
     CDS             complement(14053..14538)
                     /gene="moaC"
                     /locus_tag="ACHY03_002379"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFS3905597.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(14541..15053)
                     /gene="moaB"
                     /locus_tag="ACHY03_002380"
     CDS             complement(14541..15053)
                     /gene="moaB"
                     /locus_tag="ACHY03_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFS3905598.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(15075..16064)
                     /gene="moaA"
                     /locus_tag="ACHY03_002381"
     CDS             complement(15075..16064)
                     /gene="moaA"
                     /locus_tag="ACHY03_002381"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFS3905599.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQYRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(16052..16196)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            16461..17369
                     /gene="yvcK"
                     /locus_tag="ACHY03_002382"
     CDS             16461..17369
                     /gene="yvcK"
                     /locus_tag="ACHY03_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFS3905600.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(17560..19581)
                     /gene="uvrB"
                     /locus_tag="ACHY03_002383"
     CDS             complement(17560..19581)
                     /gene="uvrB"
                     /locus_tag="ACHY03_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFS3905601.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         4770 a         5184 c         5116 g         4763 t
ORIGIN      
        1 tatttataga gatcacattt tcatgtctgg attgcccggt tttaatcgtc atattgatat
       61 catttgcgct gatctccgaa atctcactga cgaatttttt aaatgcatta tagcgctggt
      121 ggccagcatt aaatttgcca aatgaaacat aatatgcgaa atgttcaagg aaaccatgta
      181 attccagtaa attaaccggg tcagaaccct gcaccatatt taataacatc gtcgcataac
      241 cattgcattt tgaacaatcc atacatagct cctttgcact tgaatgatct gcgaatcata
      301 agcctggcaa aatggggaat cttacagaaa tcgctcattt ttaacttttc gtactgcaaa
      361 ctcaggatca accgggttat gaaagtctgc tgctgtacgg ctctaaacag actggcaatc
      421 aatctttcta tcaataagct gaaatgagcg atttttgata gtttattaga actactaaag
      481 atatacggta atgaaacgac tgcacacttt attttgctta gggagatagt cggatacact
      541 atagacaatg aaaataataa aatcagggac agaatcaagc taatactgaa tcacatatct
      601 cataataatt taccagatga tgaacaaact ctttatgcaa tagcatgttt attccagatt
      661 ttcccagaaa ttgtttatga atatcaacca atgttattaa caataattca aattgcaaga
      721 agaaataatt tcattgccat tttagataca ctagatagat cataaagcaa atttattaaa
      781 acaataaaat cagacatgtc atgcatgtct gaccgtgtta cctgaataat gggcaaaagc
      841 ctgtgatact cttttgccct tacatggaat tactgcgcag cggcaggttt acgcggacgg
      901 cggcgacgtt gttgttcgcc tgccggtttg gcatcgccga ggcggcgaga cggtttttct
      961 gcaggcttcg cgcttgcaga ttttgcacca ccttctccac ggcgtagttg ctgttgaccg
     1021 cgaccaccgc cctgcccacg accaccgccg ccacgttgct ggcgaccgtt ctggatcggt
     1081 tcggctttga ttgacgggtc cggctcatag cccggaatcg caatgcgcgg gatctctttt
     1141 ttcagcagtt tttcgatatc acgcagcagt ttgtgttcat caacacacac caacgacaac
     1201 gcttctccgg tggctgcggc acgaccggta cgcccgatac ggtggacata atcttccggt
     1261 acgtttggca gttcatagtt aacgacgtgc ggcaactctt cgatatccag accgcgcgca
     1321 gcgatgtcag ttgccaccag tacacgaata tcgcccgatt taaaatcggc cagcgcacgg
     1381 gtacgcgcac cttgcgattt attaccgtgg atcgccgcac tacggatgcc atctttatta
     1441 agctgttcag cgagatggtt agcgccatgt ttggtacggg taaacaccag cacctgctgc
     1501 cagttccctt tcccaatcat gtgcgacagc aattcgcgct tgcgtttctt atcaacaaag
     1561 tgaacatgct gggtcacctg atcagacgcg gtattgcggc gtgccacttc gatttccagc
     1621 gggttgtgca acagtttttc cgccaggact ttaatatcgt cagagaaggt cgcggagaac
     1681 aacaggttct ggcgcttcgc aggtagtttt gttaacacgc gacggatatc gtggataaag
     1741 cccatgtcga gcatgcggtc agcttcatcg aggacgagga tttcaacctg atccagcttc
     1801 actgcattct gatgttccag gtccagcaaa cgtcccggcg ttgccaccag cacatcaacg
     1861 ccgccgcgca acttcatcat ttgtgggtta atgctgacac cgccaaacac caccagcgaa
     1921 cgaatgttca ggtatttgct gtaatcacgg acgttttcgc caatctgcgc agccagttca
     1981 cgggtcgggg taagaatgag agcacgtacc ggacgacgcc ctttggcgtg cggctggcga
     2041 gtgatcaggt gttgcaacag cggcagcgta aagcccgctg ttttgccggt gccggtctgg
     2101 gcgctagcca tcaggtcgcg gccttccagc accgcaggga tcgcctgcag ctgaataggg
     2161 gtgggttcac ggtaaccctg ctcggcaacg gcgcgcagga tatcagggct taaacccaaa
     2221 gaatcgaaag acataactac tccgaaccgc cccgaccgtt accggtgtag ttttcaggga
     2281 gatactgaaa aggaaaatga caaaaaccac aatgtcatga gggagcggag tgtagcagtt
     2341 tttgtgacgc agcgcataaa ttatccctgt gactggcgta gctaaaattc ttagccatac
     2401 tggacaactc ccctatggag tcataatctt aatcaatcat ttgattaaga atggactgtg
     2461 cgatgaataa tcctgccatg acaatcaagg gtgaacaggc gaaaaaacag ctgattgctg
     2521 ccgcactggc gcagtttggt gaatatggaa tgaacgccac cacccgcgag atagccgccc
     2581 aggccgggca gaatatcgct gccatcacct actacttcgg ttcgaaagaa gatttgtacc
     2641 tcgcctgcgc ccagtggatt gccgatttta ttggcgagca gttccgtccg catgccgagg
     2701 aagccgaacg cttgttcgcc caaccacagc ctgatcgggc tgccatccgt gaactgatcc
     2761 ttcgcgcctg caggaacatg attaagctgc tcacccagga tgacaccgtc aacctcagca
     2821 agtttatctc ccgtgagcag ctctctccca cggcagccta ccacctggtg cacgaacagg
     2881 tgattagtcc gctacacagc cacctgacac gcctgattgc cgcctggacc ggctgcgacg
     2941 ccaatgacac ccgcatgatc cttcataccc atgcgttgat tggtgagatt ctggcgttcc
     3001 gtcttggcaa agaaacaatt ctgttacgta ccggctggac cgcgttcgat gaagaaaaga
     3061 ccgaactgat caaccagacg gtgacctgtc atatcgacct gattttgcaa ggattatcgc
     3121 aaaggagttt gtagtgatga aaaaacctgt cgtgatcgga ctggcggtag tggtacttgc
     3181 cgccgtggtt gccggaggct actggtggta tcaaagccgc caggataacg gcctgacgct
     3241 gtatggcaac gtggatattc gtacggtgaa tcttagtttc cgtgttgggg ggcgcgttga
     3301 atcgctggcg gtggacgaag gtgatgcgat caaagcgggc caggtgctgg gcgaactgga
     3361 tcacaagccg tatgagattg ccctgatgca ggcgaaagcg ggtgtttcgg tggctcaggc
     3421 gcagtatgac ctgatgcttg ccgggtatcg cgatgaagaa atcgctcagg ccgccgctgc
     3481 ggtgaaacag gcgcaagccg cctatgacta tgcgcagaac ttctataacc gccagcaagg
     3541 gttgtggaaa agccgcacca tttcggcaaa tgacctggaa aatgcccgct cctcgcgcga
     3601 ccaggcgcag gcaacgctga aatcagcaca ggataaattg cgtcagtacc gttccggtaa
     3661 ccgtgaacag gacatcgctc aggcgaaagc cagcctcgaa caggcgcagg cgcaactggc
     3721 gcaggcggag ctgaatttac aggactcaac gttgatagca ccgtctgatg gcacgctgtt
     3781 aacgcgcgcg gtggagccag gcacggttct caatgaaggt ggcacggtgt ttaccgtttc
     3841 actaacgcgt ccggtgtggg tgcgcgctta tgttgatgaa cggaatcttg accaggccca
     3901 gccggggcgc aaagtgctgc tttataccga tggtcgcccg gacaagccct atcacgggca
     3961 gattggtttc gtttcgccga ctgctgaatt taccccgaaa accgtcgaaa caccggatct
     4021 gcgtaccgac ctcgtctatc gcctgcgtat tgtggtgacc gacgccgatg atgcgttacg
     4081 ccagggaatg ccagtgacgg tacaattcgg tgacgaggca ggacatgaat gatgccgtta
     4141 tcacgctgaa cggcctggaa aaacgctttc cgggcatgga caagcccgcc gtcgcgccgc
     4201 tcgactgtac cattcacgcc ggttatgtga cggggctggt ggggccggat ggtgcaggta
     4261 aaaccacgct aatgcggatg ctggcgggat tactgaaacc cgacagcggc aatgccacgg
     4321 tgattggctt tgatccgatc aaaaacgacg gcgcgctgca cgccgtgctc ggctatatgc
     4381 cgcagaaatt tggtctgtat gaagatctga cggtgatgga gaacctcaat ctgtacgcgg
     4441 atttgcgcag cgtcaccggc gaggcacgta agcaaacttt tgctcgcctg ctggagttta
     4501 cgtctcttgg gccgtttacc ggacgcctgg cgggcaagct ctccggtggg atgaaacaaa
     4561 aactcggcct ggcctgtacc ctggtgggcg aaccgaaagt gttgctgctc gatgaacccg
     4621 gcgtcggcgt tgaccctatc tcacggcgcg aactgtggca gatggtgcat gagctggcgg
     4681 gcgaagggat gttaatcctc tggagtacct cgtatctcga cgaagccgag cagtgccgtg
     4741 acgtgttact gatgaacgaa ggcgagttgc tgtatcaggg agaaccaaaa gccctgacac
     4801 aaaccatggc cggacgcagc tttctgatga ccagtccaca cgagggcaac cgcaaactgt
     4861 tgcaacgcgc cttgaaactg ccgcaggtca gcgacggcat gattcagggg aaatcggtac
     4921 gtctgatcct caaaaaagag gccacaccag acgatattcg ccatgccgac gggatgccgg
     4981 aaatcaacat caacgaaact acgccgcgtt ttgaagatgc gtttattgat ttgctgggcg
     5041 gtgccggaac ctcagaatcg ccgctgggtg caatattgca tacggtagaa ggcacacccg
     5101 gcgagacggt gatcgaagcg aaagaactga ccaagaaatt tggtgatttt gccgccaccg
     5161 atcacgtcaa ctttgccgtt aaacgtgggg agatttttgg tttgctgggg ccaaacggcg
     5221 cgggcaaatc gaccaccttt aagatgatgt gcggtttgct ggtgccgact tccggccagg
     5281 cgctggtgct ggggatggat ctgaaagaga gttccggtaa agcgcgccag catctcggct
     5341 atatggcgca aaaattttcg ctctacggca acctgacggt cgaacagaat ttacgctttt
     5401 tctctggtgt atatggctta cgcggtcggg cgcagaacga aaaaatctcc cgtatgagcg
     5461 aggcatttgg cctgaaaagt attgcctcac acgccaccga tgaactgcca ttaggtttta
     5521 aacagcggct ggcgctggcc tgttcgctga tgcatgaacc ggatattctg tttctcgacg
     5581 aaccgacttc cggcgttgac cccctcaccc gccgtgaatt ctggctgcac atcaacagca
     5641 tggtagagaa aggcgtcacg gtgatggtca ccacccactt tatggatgaa gcggaatatt
     5701 gcgaccgcat cggcctggtg taccgcggga aattaatcgc cagcggcacg ccggacgatt
     5761 tgaaagcaca gtcggctaac gatgagcaac ccgatcccac catggagcaa gcctttattc
     5821 agttgatcca cgactgggat aaggagcata gcaatgagta acccgatcct ttcctggcgt
     5881 cgcgtgcggg cgctgtgcgt caaagagacg cggcagatcg ttcgcgatcc gagtagctgg
     5941 ctgattgcgg tagtgatccc gctgttgctg ctgtttattt ttggttacgg cattaacctc
     6001 gactccagca agctgcgggt cgggatttta ctggaacagc gtagcgaagc ggcgctggat
     6061 ttcacccaca ccatgaccgg ttcgccctac atcgacgcca ccatcagtga taaccgacag
     6121 gaactgatcg ccaaaatgca ggcggggaaa attcgcggtc tggtggttat tccggtggat
     6181 ttcgccgaac agatggagcg cgccaacgcc accgcaccga ttcaggtgat caccgacggc
     6241 agtgagccga ataccgctaa ctttgtgcag gggtatgtcg aagggatctg gcagatctgg
     6301 caaatgcagc gagcggagga caatgggcag acttttgaac cgcttattga tgtacaaacc
     6361 cgctactggt ttaacccggc ggcgattagc cagcacttta ttatccccgg tgcggtgacc
     6421 atcatcatga cggtcatcgg cgcgattctc acctcgctgg tggtggcgcg agaatgggaa
     6481 cgcggcacta tggaggctct gctctctacg gagattaccc gcacggaact gctgctgtgt
     6541 aagctgatcc cctattactt tctcgggatg ctggcgatgc tgctgtgtat gctggtgtca
     6601 gtgtttattc tcggcgtgcc gtatcgcggg tcgctgctga ttctgttttt tatctccagc
     6661 ctgtttttac tcagtaccct ggggatgggg ctgctgattt ccacgattac ccgcaaccag
     6721 ttcaatgccg ctcaggtcgc tctgaacgcg gcttttctgc cgtcgattat gctttccggc
     6781 tttatttttc agatcgacag tatgcccgcg gtgatccgcg cggtgacgta cattattccc
     6841 gcacgttatt tcgtcagcac cctgcaaagc ctgttcctcg ccgggaatat tccagtggtg
     6901 ctggtggtga acgtactgtt tttgatcgct tcggcggtga tgtttatcgg cctgacgtgg
     6961 ctgaaaacca aacgtcggct ggattaggga gaagagcatg tttcatcgct tatggacgtt
     7021 aattcgcaaa gagttgcagt cgttgctgcg cgaaccacaa acccgcgcga ttctgatttt
     7081 acccgtgcta attcaggtga tcctgttccc gttcgccgcc acgctggaag tgactaacgc
     7141 caccatcgcc atctacgatg aagataacgg cgagcattcg gtggaactta ctcaacgttt
     7201 tgcccgcgcc agcgccttta cacatgtgct gctgctgaaa agcccacagg agatccgccc
     7261 aactatcgac acacaaaagg cgttactcct ggtgcgtttc ccggctgact tctcgcgcaa
     7321 actggatacc ttccagaccg cgccgttgca gttgatcctc gacgggcgta actccaacag
     7381 tgcgcaaatt gccgccaact acctgcaaca gatcgtcaaa aattatcagc aggagctgct
     7441 ggaaggaaaa ccgaaaccta acaacagcga gctggtggta cgcaactggt ataacccgaa
     7501 tctcgactac aaatggtttg tggtgccgtc gctgatcgcc atgatcacca ctatcggcgt
     7561 aatgatcgtc acttcacttt ccgtcgcccg cgaacgtgaa caaggtacgc tcgatcagct
     7621 actggtttcg ccgctcacca cctggcagat cttcatcggc aaggccgtac cggcattaat
     7681 tgtcgccacc ttccaggcca ccattgtgct ggcgattggt atctgggcgt atcaaatccc
     7741 cttcgccgga tcgctggcgc tgttctactt tacgatggtg atttacggtt tatcgctggt
     7801 gggattcggt ctgttgattt catcactctg ctcaacacaa cagcaggcgt ttatcggcgt
     7861 gtttgttttt atgatgcccg ccattctcct ttccggttac gtttctccgg tggaaaacat
     7921 gccagtatgg ctgcaaaacc tgacgtggat taaccctatt cgccacttta cggacattac
     7981 caagcagatt tatttgaagg atgcgagtct ggatattgtg tggaatagtt tgtggccgct
     8041 actggtgata acggccacga cagggtcagc ggcgtacgcg atgtttagac gtaaggtgat
     8101 gtaacttctt atctttcgcc agcaaagaca ctaccgccgg gccggcaagg attgccagcc
     8161 ctgcaattgc cagcaaaaag ttgttttcca gtacgcggga caacagccac agcacaatca
     8221 gcgctgcgcc aaaataccag gtcgtggtca gctcttccag cacgtctgcc acctcacgcc
     8281 agcgttgctc ggggtggcgc tgaaacacca gcatcatcac taccgtgaca caatacagtg
     8341 cgcataatcc cacgccgacg tgaaccagag tcttactcat ccagcccacc accagcagcg
     8401 ccactaccag taaatgcaac ataatctgcc agcaacttag acccgttgcg acacgaacac
     8461 gttgttgcca cttcatggct tctctcctga agaatttatc tctacttact cagagtaccg
     8521 aactttacgg aaaattccgt aagaccgcac ctttttgtga cgccgactac actatttcaa
     8581 tgagataaga ggcaaagaca ggaaaattat gcccgatcaa acacaacaat tttcgttcaa
     8641 ggtgctcacc atcaatattc acaaaggctt taccgcgttt aaccgacgct tcattttgcc
     8701 ggaacttcgc gacgccgttc gtaccgtcag cgccgatatt gtttgcttgc aggaagtgat
     8761 gggcgcgcac gaagttcatc cgctgcatgt ggaaaactgg cccgatacct cgcactacga
     8821 gtttctcgcc gacaccatgt ggagcgattt tgcctacggt cgcaatgccg tatacccgga
     8881 agggcatcac ggtaacgccg tactgtcgcg ctatcccatt gaacattatg agaatcgcga
     8941 tgtttcggtc gatggtgcgg aaaagcgcgg cgtgctctac tgccgcattg tgccgccgat
     9001 gacccgaaaa gcgattcatg tgatgtgcgt acatctgggc ctgcgtgagg cgcaccgtca
     9061 ggcgcagctt gcgatgctcg ccgaatgggt gaatgagcta ccggacggcg aaccggtatt
     9121 ggtggcgggt gatttcaatg actggcggca aaaagctaat catccgttaa aagtgcaggc
     9181 cggactggat gagattttta cccgcgccca cggacgcccg gcgcgcacgt ttccggtgca
     9241 atttcctcta ctacgactgg acaggatcta cgtcaaaaat gccagcgcca gcgcgccaac
     9301 cgcgttgccg ctgcggacat ggcgacacct ttctgatcat gcccctttaa gtgcggagat
     9361 tcatttatga aatgtagctg gcgcgaaggc aataagatcc agttgctgga aaacggcgag
     9421 caatattatc ccgcggtgtt taaggcgatt ggcgaggcgc aagaacgcat cattcttgaa
     9481 acgtttatct ggtttgagga tgacgtcggc aaacagctgc atgcggcgct actggcagca
     9541 gcacaacgcg gggttaaagc ggaagtcttg ctggatggct acggttcgcc ggatctcagc
     9601 gatgagtttg tcaatgaact gacggcagct ggcgtggtat ttcgctacta cgatccccgc
     9661 cctcgccttt ttggtatgcg caccaatgtg tttcgccgga tgcatcgcaa aattgtggtg
     9721 atcgacgcgc gtatcgcctt tattggcggg ctgaattact ccgccgagca tatgtccagc
     9781 tacggtccag aggccaaaca ggattacgcg gtacgccttg aagggccgat tgttgaagat
     9841 atcctccagt ttgaactgga aaatctgcca ggacaaagcg cggcccgacg ctggtggcga
     9901 cgtcatcaca aagcggaaga aaatcgccag ccgggagaag cgcaggtatt gctggtatgg
     9961 cgcgataacg aagaacatcg cgatgatata gaacgccact atctgaaaat gctcactcag
    10021 gcgcggcggg aagtcattat cgccaacgcc tacttcttcc ccggctatcg atttttacac
    10081 gccttgcgta aagcggcacg gcgcggggtg cggatcaaac tgatcattca gggcgaaccg
    10141 gatatgccga ttgtcagagt cggtgcgcgc ttgctgtata actatctggt taaaggcggc
    10201 gttcaggtat ttgagtaccg ccgccgcccg ctccacggca aagtggcatt gatggacgat
    10261 cactgggcga cagtagggtc cagtaatctc gatccgctca gtttgtcact gaatctcgaa
    10321 gctaatgtca tcatccacga tcgtcatttt aaccagacgc tgcgcgataa tctgaacggc
    10381 attatcgccg cagattgtca gcaggtcgat gagaccatgc tgccgaaacg cacctggtgg
    10441 aacctaacca aaagcgtgct ggcgttccac tttttacgcc acttcccggc gctggttggc
    10501 tggcttccgg cacacacgcc acgtctggcg caggttgatc cccccgcaca accgacaatg
    10561 gaaacgcagg atcgggtaga aactgaaaac acgggggtaa aaccctgatg agtaaatcac
    10621 acccgcgctg gcgcttagca aagaagatcc tcacctggct gttttttatc gcggtgattg
    10681 tgttgctggt ggtctacgcc aaaaaagtgg actgggaaga ggtctggaag gtcatccgcg
    10741 actacaatcg cgttgcgctg cttagtgcgg tcgggctggt ggtcgtcagc tatctgattt
    10801 acggctgcta tgacctgctc gcccgctttt actgcggtca caaactggcg aagcgccagg
    10861 tgatgctggt gtcgtttatc tgctacgcct tcaacctgac actcagtacc tgggtcggcg
    10921 gcattggtat gcgctatcgt ttgtactctc gtctggggtt gcccggtagc actattacgc
    10981 ggattttctc gctcagtatt accaccaact ggctgggcta tattttgctg gcggggatta
    11041 tctttaccgc aggcgtggtg gagctaccgg atcactggta tgtcgatcaa accacactgc
    11101 gcattctcgg cattggctta ctgatgatta tcgcggttta tttgtggttt tgcgctttcg
    11161 cgaagcaccg ccatatgacc atcaaagggc aaaagctggt gctgccctca tggaaattcg
    11221 ccctcgccca gatgttgatt tccagtgtta actggatggt catgggagcg attatctggc
    11281 tgttacttgg tcaaagcgtg aactatttct ttgtactggg cgtgttgctg gttagtagta
    11341 ttgcaggcgt catcgtgcat attccggcgg ggattggtgt gctggaagcg gtatttatcg
    11401 cattactggc aggggagaat acatccaaag gcacaattat cgccgccctg ctcgcttatc
    11461 gcgtcctgta ttactttatc ccgctgctgc tggcgctgat ctgttacctg ctactggaaa
    11521 gccaggcgaa gaagctacgg gcgaaaaatg aagcggcgat gtgaagttca gttaagttct
    11581 ttagtatgtg catttacgac taatgaaaaa aacgcgtatg cctttgccag acaagcgtta
    11641 tagctatcgc ggcaagtatc ccaaagaagc cgataaaata gtaatagagt gttaatgcac
    11701 ctataaaagc gcaacgaacc ggactccaca actcttcact atggcatttc cccgccagtt
    11761 caaggagctt atttcgtcca tgcgagataa tgcctgacca taacacaatc attaagcaac
    11821 ttattaccca aataggccgt tcactcatta atgccgcatt cacgatgatt accagggcca
    11881 accctgtcaa cgtcatcata atgataaaac ggtgagaacc aggatcaaca ttaaataata
    11941 aaccggccag catactgatt aaaaacatcg cgcccgcagt gctgaacacg atgataatgg
    12001 catatacagg aactaaactt gcaacgacaa tcgccaccaa agagagtaaa aacagatata
    12061 ttacgcctgc gatggcggca tttatcttgt tgatatcctt acttaagcca tacatacaaa
    12121 gtaataataa ccctatggcg acatacattg gtggtgaatc atatttggtt gccgtaattt
    12181 cgagatactt agcacctcag gccagccctg cggttgccac caaacctaaa gcggtaatga
    12241 gccatatacc gtttaatatc ctggtctcgt gctgaaaatc taatttattg ctgttttgca
    12301 agtatgattc cattctttta gttgtcctta tagaaaaata ttaatattca tcatgaataa
    12361 atttaaaccc acgcatacaa aatacttttg tatttatgga tacggaaaac tcttccgctc
    12421 ggccataagg cgtattttat acctggtact tgttaagtgt cgtttttagt aagaaaaaaa
    12481 atcccgacac atggccggga tattgatggt gaaaaaagaa ttaacgacga ttgccaaaaa
    12541 ttcgcagcaa catcaggaac aggttgatga agtccagata caaggttaac gcgccaagaa
    12601 tggaatattt acgcaggtta gagctgtcgc gggtatcaat ctgctcaccc atgtttttca
    12661 gtttctgggt gtcgtatgcg gtcagcccca caaaaacaat cacaccgata taggtgacgg
    12721 cccacatcaa tgcttcgctt ttcagccaga agttgaccag cgatgccagc acaatgccga
    12781 ttaacgccat aaacagcata ttgccaaagc cacttaaatc gcgcttcgtg gtgtaaccgt
    12841 acaggctcat tgcgccgaac atcccggcag taacgacgaa ggtactggcg atagaagcag
    12901 cggtataaac aatgaaaata ctggaaagcg taagacccgt cagcgccgaa taaagcataa
    12961 agagcatcgt cgttacacct gcgctcagct tttgaatcat cgctgataac acaataacca
    13021 atgctaattg cgcgatgatc agaccgatta aaaagacacg gttagtgaac aacagctcca
    13081 tcacggccgc ggaattagcc gcataccagg caacaaatgc ggtcagcaac aagccaacgg
    13141 tcatccagcc atagacttgc gccatataag tctgcaagcc agcccggggt tgtacaattg
    13201 aatcagaacg tgggaatctg tccatgacga tctcctgaag atataaggaa tatcttaagg
    13261 atactgcaaa atgatgaggc tgtgcatcga cgcagcgtaa acgcatgtac tgagcggcaa
    13321 aattgccgga tgcagcggtg ccttatccgg ctaaaaaaaa ctaccagcgt tttgctgcct
    13381 gctgatcgct ctcccgagct tcgacccagc ggtcgccttc aggcgtggct tcgcgcttcc
    13441 agaacggtgc gcgggttttg agataatcca taataaactg cccggcttca aacgcactgt
    13501 tgcgatgcgc actggtgaca ccgacaaaaa cgatttcatc gcccggccat aattccccga
    13561 tgcggtgaat caccgtgacg cgcccaagcg gccatcggtt acgcgcttca tcgacaattt
    13621 ctgccagtgc tttttcagtc atccccggat agtgttcgag ggttaatgct ttgacgctgt
    13681 cgcccaggtt atggttgcgc accttaccgg taaaggtgac taccgcaccg tcttcgtcac
    13741 gctccgccag ccacgggtac tcttctccta cgctgaacgg ctgcggacca acaacaattt
    13801 tggtttctgc catcttaacc tccggttacc ggcgggaaga aagctacttc gtcgccgtca
    13861 gtcagcggat ggtcaaaact caccagcgtc tggttgacgg cagccagtaa tttgccatct
    13921 tccagcgcca gcgcccagcg attgctctgc gcagccaggt gttggcgtaa cgcttcaacg
    13981 gttgggaaat ccgcagccac ttcggttgca tctgttccta ccaactcgcg cacctgggcg
    14041 aaaaaaagaa ctttaatcat ccgcttccac cttaaagtca cccgacttgc cgccgctctt
    14101 cgccagcaaa cgtaccggac caatcaccat atctttttgc accgctttgc acatgtcata
    14161 aatggtcagc gccgccacgg aggccgcggt taacgcttcc atttcgacac cggttttccc
    14221 ggtcaggcgg cataaggttt ctatacgcac ccgattgtgc tccggctcgg cctgtaaatt
    14281 gacttcaact ttgctgagca tcagcggatg acacagcggg atcagatccc aggtgcgttt
    14341 tgccgcctga ataccggcaa tacgcgcagt ggcaaatacg tcgcctttgt ggtggcgacc
    14401 atcaataatc atcgccagcg tctcgctgcg catggtgaca aaggcttcgg cgcgcgcttc
    14461 acgcacggtt tccgctttgg cggagacatc caccatgtgc gcttcgccag cggcgttgat
    14521 atgggtcagt tgcgacatac ttatttcttc aaatgtggat ggaaattaca cggacgcgta
    14581 cgggcatcca gctgcggcgc gatgatattt tcccatgcgg tacggcacgc tttggtcgaa
    14641 cccggcattg cgaaaatcag cgttttgtta gcgacgcccg ctaccgcacg agattgcaac
    14701 gtggaagtgc caatctcttc aaacgacaac atacggaaca cttcaccaaa accttcaact
    14761 tcacggtcga acaacggcag caatgcttcg ggagcctgat caccttccgt caggccagta
    14821 ccacccgtaa tcaacaccac ttgtacatcg tcgctggcga tccacgcaga tacctgagcg
    14881 cgaatagcgt agcggttttc tttcacaatg gctttatcga caacgtgatg gcccgcttct
    14941 tgcgccgaat cgcgcagata gtgaccggag gtatcgtctt cttcaccgcg acgattagaa
    15001 accgtaagaa tagcaatacg ggtcgggata aattcagtgc ttacctgact catctgatct
    15061 ctccttttga cgttttagcc gccaatgtac gataagtttt gcgtaatacc ggtgttgttt
    15121 tgatgcagga aatgggtctg tttcttctcc cgcagcgccg ctgaaatacg cgcttccagc
    15181 gcctgttgct gggtatcgtc ttccagcaga tcgcgcaggt taacgccgcc ttcaccaaac
    15241 aggcagagat ggagtttacc aatggaggaa acgcgcaggc ggttgcaagt ggcgcagaag
    15301 tctttttcat acggcatgat aaggccaatc tctccggcgt aatccggatg acaaaagact
    15361 tgcgcgggac cgtcgctgcg ttgacgtaat tggtggatcc agccgcgacg cagtagctcg
    15421 tcacgcagaa cctgaccaga gatgtgatgc ttacggaaaa gctcgctgcc ctcgcccgtt
    15481 tccatcagtt cgatgaaacg cagctggata gggcgatact ggatccagtt cagaaaggtg
    15541 tcgagctggt gatgattcac atcacgcatc agcacggtat tgactttgac cttctcaaaa
    15601 ccggcctcaa atgcagcatc gatccctgcc atcacctgat tgaatttatc ctggccggta
    15661 atggcatgaa actggcgggc gtccagactg tcgacgctga cgttaatgcc agtaagtccc
    15721 gcatcgcgcc agttcgccac atcgcgttcc agacggtaac cattggtggt gacagcaatc
    15781 tggcgaatag cgtcgttttc tcgcacagcg gcgatgatat cggtaaagtc gcggcgtaaa
    15841 gacggctcac cgcctgtcag gcgcactttt tcggtgccca gactggcgaa ggcgcgcgta
    15901 acccggcgaa tttcatcgac ggtcagaaag cctttattgg tgacgccgct cggtttgtag
    15961 ccatccggca ggcagtaggt gcaacgaaag ttacacacat cggtaatcga caggcgcaag
    16021 tagtaaaact tacgcgcaaa tgcatcagtc agttgtgaag ccatgtacac ctttccagat
    16081 acgggaggcg aagtcatttc ttccttcgcc ctggtggcaa tctttacgag cattgtcacg
    16141 gccaaagcac cgtatcagtt gacccaggtg cagaggctag agtgtttagt ggttaagccg
    16201 atactagcgt gtaaatgaga tttttaccat ctacactttc gctatgtaat catgtatata
    16261 gcgtcatgat cgtgctattt cgctacagaa taagggaaaa tggcggtttt actttgatat
    16321 acatcatcag ggaggcatag cgtgcatcgt cctttagagg tagaagaaaa tagtttgtat
    16381 cctcaatatt ggcaggttaa ttgctgtttc cccgcaattt gcgctactgt agcgcggata
    16441 atctgactcc aggaaactat atgcgcaatc gtacgctggc tgatcttgat cgtgtcgttg
    16501 ctctcggcgg agggcatgga ctgggacgcg ttctctcatc actttcgtct ttgggttctc
    16561 gtttaacggg tatcgtcacc accacggata atggtggctc gacggggcgt attcgccgtt
    16621 cagaaggcgg cattgcctgg ggcgatatgc gcaactgcct taaccagctg ataactgaac
    16681 ccagcgtcgc ttccgcaatg tttgaatatc gtttcggcgg taatggcgaa ctttccgggc
    16741 acaaccttgg aaacctgatg ttgaaggcac tggatcacct tagcgtgcgg cctcttgaag
    16801 cgattaatct gattcgcaat ctgctgaaag tggacgcaca tttgattccg atgtcggagc
    16861 atcccgttga tctgatggcg attgacgatc aggggcatga agtttacggc gaggtcaata
    16921 tcgaccagtt aactacgccg attcaagagt tattgttaac gcctaatgta cccgcaacgc
    16981 gtgaggcggt tcacgctatc aatgaagcgg atctcatcat tattgggcct ggcagttttt
    17041 ataccagcct gatgccaatt ctgctgctga aggaaatcgc acaggcatta cgccgcacgc
    17101 cagcgccgat ggtctatatc ggcaatctgg ggcgtgagtt gagtttacct gcggctaatc
    17161 tgaagctgga aagcaagctg gcaattatgg agcagtatgt tggtaaaaag gtcattgatg
    17221 cggtcatcgt cgggccaaaa gtggatgtct cggcggtgaa agagcggatt gtgattcagg
    17281 aggtgctgga ggccagcgat attccgtatc gtcatgaccg ccagttgttg cataacgcac
    17341 tggaaaaggc gttacaggct ttaggttaac ggaaatccga aggaaaattc cggcttccta
    17401 ttgaagacaa agtgcgcgtt gtttatgccg gatgcggcgt gaacgcctta tccggcctac
    17461 aaaccgcgct aattcaatgt attgcagaat catgtaggcc tgataagcgt agcacatcag
    17521 gcaattttgc ttttgtcatc tgtcttcttc gttatcctgt cacgaggccg cgataaacaa
    17581 ttcacgcaac tgatgcaact ggtcacgaat ttgtgccgct tcttcgaact ccagattttg
    17641 cgcgtgttgc atcatcaacc cttccagttc atggatcttc tgctgcaacg ctttaggcga
    17701 catatccatc ggcacattat ccggctcaac aatcgggcgc gattttcctc tgcccttcgc
    17761 tttggtttta gcaatgttct gccccaacgc caggatatcg accactttct tgttcaagcc
    17821 ttgcggcgta atgccgtgtt cctcgttgta cttctgctgt ttctcgcggc gacgttcggt
    17881 ttcgccaatc gctttcgcca ttgatggggt gatcttatcg ccgtagagaa tcgctttacc
    17941 gttaacgtta cgtgccgcac gaccaatggt ctggatcaac gaacgttcgg aacgcaggaa
    18001 gccttctttg tcagcgtcga ggatcgccac cagcgaaacc tccggcatat ccagaccttc
    18061 gcgcagtaag ttgatcccca ccagcacgtc gaactcaccc agacgcaagt cgcggataat
    18121 ctccatacgt tcgacggtgt cgatatccga gtgaagataa cgcacgcgct cgccatgttc
    18181 ttcgagatat tcggtgagat cttccgccat ccgcttggtc agtgtggtga ccagtacgcg
    18241 ttcgttaatt gccgctcgct gacgaatctc cgaaagaaga tcatcaacct gtgtcgccac
    18301 tggccgcact tcgataatcg gatcaagcaa tccggttgga cgcaccacct gatccaccac
    18361 atcgccgccg gatttttcca gctcataatt acctggcgtc gccgaaacat agatggtttg
    18421 cggcgctaat gcttcgaact cttcaaattt cagcggacgg ttatccagcg ctgatggcag
    18481 gcggaagccg tattccacca gtgtctcttt acgcgcccgg tcaccgcgat acatgccgcc
    18541 aatttgtgga atggtgacgt gagattcatc gaccaccagc agcccatcgg caggcaggta
    18601 atcaaacagc gtcggcggtg gctcacccgg tccacgacca gagaggaagc gcgagtagtt
    18661 ttcaatcccc gaacagtagc ccagctcgtt catcatctcc agatcaaact gggtacgctg
    18721 ggtcagccgc tgctcttcca gcagtttgtt gttttccaat agcactttgc gtctggcggc
    18781 cagctcttct ttgatctcct ccatcgcctg tacgatgcgc tcgcgcggtg tgacgtagtg
    18841 cgttttcggg tagatggtaa aacgtggaat ggtggaaaca atctgcccgg ttagcgggtc
    18901 aaataacgac aatcgttcca cttcctcgtc aaacagttcc acgcgaagtg caatgtcatc
    18961 cgattctgcc gggaagatat caatcacctc gccacgaaca cggaaagtac cacgctggaa
    19021 tgcttgatca ttacgagcgt attgcagctc cgccagtcgg cgcagaatcg cgcgctgatc
    19081 gataatcata ccgaccgtga gatggagcat catcttgaga tataaatcag gatcgcccag
    19141 accataaatc gcggaaacag acgcgaccac aaccacatca cgccgctcca gcatcgcttt
    19201 ggtggcggac aaacgcatct gttcgatatg ttcgtttacc gaggcatctt tctcaatgaa
    19261 ggtgtcggaa ctcggtacat aggcttctgg ctgatagtag tcgtagtagg agacgaaata
    19321 ttccaccgcg ttttccggga agaactcttt catttcgcca tacagctggg ccgccagcgt
    19381 tttgttgggc gcaagtacca tggtagggcg ctgaaggtca gcaatgacat tggcaatggt
    19441 gaaggttttc ccggaaccag tcacaccaag taacgtctgg tgcgccaggc catcttccag
    19501 cccctcttcg agacgtcgaa tcgcctctgg ctgatcgcca gaaggtttaa aagcggaatt
    19561 cagtttgaac ggtttactca tgagtcgcta cctgaaggag ttgggcgggc aggtatgtaa
    19621 ttttactcgt cgtacttaat tatgccaaca aattatactg gataaaaaaa cagttcatca
    19681 ccataatatt tctgttacag cgtaaactcc gctgtcaatc atgagcaaaa tttactctgt
    19741 ggcgagataa aactcggacc ttaccgggtt atccccaaag caacggcttt tttaacattt
    19801 gtcaagatga gtgatgtcag ttttatggca aga
//
LOCUS       DAZUCE010000079        19531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZUCE010000079 DAZUCE010000000
VERSION     DAZUCE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.079"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            228..728
                     /gene="tssB"
                     /locus_tag="ACHY03_002384"
     CDS             228..728
                     /gene="tssB"
                     /locus_tag="ACHY03_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFS3905602.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQSAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            763..987
                     /locus_tag="ACHY03_002385"
     CDS             763..987
                     /locus_tag="ACHY03_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905603.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1038..2513
                     /gene="tssC"
                     /locus_tag="ACHY03_002386"
     CDS             1038..2513
                     /gene="tssC"
                     /locus_tag="ACHY03_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFS3905604.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWCSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2520..2933
                     /gene="tssE"
                     /locus_tag="ACHY03_002387"
     CDS             2520..2933
                     /gene="tssE"
                     /locus_tag="ACHY03_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFS3905605.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2937..4787
                     /gene="tssF"
                     /locus_tag="ACHY03_002388"
     CDS             2937..4787
                     /gene="tssF"
                     /locus_tag="ACHY03_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS3905606.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFNLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4751..5833
                     /gene="tssG"
                     /locus_tag="ACHY03_002389"
     CDS             4751..5833
                     /gene="tssG"
                     /locus_tag="ACHY03_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFS3905607.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5858..7138
                     /gene="tagH"
                     /locus_tag="ACHY03_002390"
     CDS             5858..7138
                     /gene="tagH"
                     /locus_tag="ACHY03_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFS3905608.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7135..7659
                     /gene="tssJ"
                     /locus_tag="ACHY03_002391"
     CDS             7135..7659
                     /gene="tssJ"
                     /locus_tag="ACHY03_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFS3905609.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPIKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7662..8993
                     /gene="tssK"
                     /locus_tag="ACHY03_002392"
     CDS             7662..8993
                     /gene="tssK"
                     /locus_tag="ACHY03_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3905610.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8998..9759
                     /gene="icmH"
                     /locus_tag="ACHY03_002393"
     CDS             8998..9759
                     /gene="icmH"
                     /locus_tag="ACHY03_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFS3905611.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9768..12527
                     /gene="tssH"
                     /locus_tag="ACHY03_002394"
                     /gene_synonym="clpV"
     CDS             9768..12527
                     /gene="tssH"
                     /locus_tag="ACHY03_002394"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFS3905612.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTEDARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPSAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESEQDNTGAVPADEAGSVQPEETAETVSPVQRLAQLTAELDAL
                     HNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLAIA
                     SLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSE
                     FQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAF
                     DKGEMADGEGRLIDCKNIVFFLTSNLGYHVIVEHADDPETMQEVLYPVLADFFKPALL
                     ARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVTRAEN
                     GARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDESVTK
                     DETVL"
     gene            12524..13267
                     /gene="vasI"
                     /locus_tag="ACHY03_002395"
     CDS             12524..13267
                     /gene="vasI"
                     /locus_tag="ACHY03_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFS3905613.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13272..14684
                     /gene="tssA"
                     /locus_tag="ACHY03_002396"
     CDS             13272..14684
                     /gene="tssA"
                     /locus_tag="ACHY03_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFS3905614.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELALATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14793..18227
                     /gene="tssM"
                     /locus_tag="ACHY03_002397"
     CDS             14793..18227
                     /gene="tssM"
                     /locus_tag="ACHY03_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFS3905615.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPSVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18238..>19531
                     /locus_tag="ACHY03_002398"
     CDS             18238..>19531
                     /locus_tag="ACHY03_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFS3905616.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQPSSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVANSRWPESLQQQQASTQ
                     WNEALKTRAQNSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRW"
BASE COUNT         4518 a         5415 c         5462 g         4136 t
ORIGIN      
        1 tgacgcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
       61 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
      121 gtggtgattt tattttcacc gccatcaagg cggcatttcc gatatttttt tcggtacgaa
      181 tatgccgtgt tcatggcctg tacgagataa catgacggat cacttttatg agcaaaaaat
      241 ttgaaggcag cgtcgcgcct cgcgagcgta ttaatatatc gtatgtccca aaaacagatg
      301 gccagtcggc tgaagtggaa cttccgctta acatgctcgt tgtcggcgat accggaaata
      361 cgcaggaaac atcctcactg gatgaacgtc aggcagtttc cgttaacaag cacaatttcg
      421 gtgcagtgat ggctgaagca gccattggcc tcaattttac cgtccccgcc acgctgaagg
      481 gaagcacaac ggacgatgag ctgaatgtgg cgctgaatat caagtcactg gatgattttt
      541 caccagatag cgtggcccgg caggttccgg aagtgaataa attgctggaa ctgcgtgaag
      601 cactgactgc actgaagggg ccgatgggta accttccggc attccgtacc cagctccagg
      661 cattgctgga aaatgaagag tcccgcgagc agttgctcaa agaaatcggc caggtgagca
      721 ataaataacc accactattt cattaaacag ggttatccag gcatgacggc acacaaaaat
      781 atctccggga cataccacct ctctgttgca gacattctgc aggtggttta ccaggtatgt
      841 ttcagcccgt ccgttgaaat caaccaggat ggtgtggctg cgctgattac aacactggat
      901 cgccgtatca gtgatctatt ggatgagatt atccattttt gtgagtttca gcagagcgcg
      961 tcgcactggc agcgcgtact tcactgacgg cgaaaagccg gtttcagaaa cacagctaaa
     1021 acagaaggat tttcagtatg tctttacagg aagaggaact cgtttccagt catgccgggc
     1081 agcctgagca ggcatcgtcg ctgcttgatc aaatcatggc acagacccgt attcagcccg
     1141 gttcggaagg ttatgacgtt gcccgccagg gtgtgacagc ctttattgcc agcatcctgc
     1201 aatccactgc atctgccgaa ccggtcaata aactggccgt ggacagcatg attgcagata
     1261 ttgatgaacg catcagccgc cagatggatg tcatcatcca tgcgcctgcc tttcagcagg
     1321 tcgagtcgtt ctggtgctca ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca
     1381 tcaaggtcaa cgtgcttcat gtcaccaagc aggaacttct ggaagacttt gagtttgcgc
     1441 cagaaattat tcagtccgga ttttataagc acgtttattc gtccggtttc ggacagtttg
     1501 gcggtgaacc catcgctgcc gtgctgggcg cttatgagtt caaaaatacc gcgcctgaca
     1561 tgaaactgct gcaatacgtc agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct
     1621 ccgtttcccc ggagtttatg gggctgaact cgtggaccga actgcctaat atcaaagatc
     1681 tttatgccat ctttgaaggt ccggcctaca ccaaatggcg cgccctgcgt gactcggaag
     1741 attcgcgcta tttagggctt acggcacccc gcttcctgct tcgccagccc tattcaccaa
     1801 cagataaccc tgttaagaac tttaactatt acgaagatgt cagccagaac cacgaagatt
     1861 atctgtgggg taatacggcc tggatgctgg catgcaatat cgcagacagt tttgccaaat
     1921 accgctggtg tccaaacatt atcggtccgc aaagcggcgg cgcagtgaaa gatctgccgg
     1981 tgcatctgtt cgaaacgatg gggcagattc aggccaaaat cccaaccgag gtgctggtca
     2041 ccgatcgccg cgaatttgaa ctggctgaag agggttttat cacccttacc atgcgtaaag
     2101 actctgataa tgcagccttt ttctctgcaa actcggtaca aaaaccgaag cactttccgg
     2161 gaaaagatgc agaaaccaac tataagctgg gtacgcagct tccttatctc ttcatcatca
     2221 atcggttagc gcactacatc aaggtgttgc agcgtgaaca actggggtca tggaaagaaa
     2281 ggagcgactt agaaagagag cttaatacct ggatccgtca gtatgttgcc gaccaggaaa
     2341 acccgcctgc agacgtgcgc agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg
     2401 atgtagaagg cgaacccggc tggtaccagg ttgcgctaag cgtgagacct cattttaaat
     2461 ttatgggggc aaattttgag ctttccctgg ttggccggtt agacagggag taacacacca
     2521 tgataccgga tcgggaaaga accacaggtt ctctttttga gcgtatggaa gcctcttcgg
     2581 ccagaaaccg gcagggaggc agtattcatt cgctcaggca atccattcgc cagaacctac
     2641 gaaacattct taatacccgg tccggtagtt gccggggtgc gcctgagctg ggcattgatg
     2701 aaccggaagg ggctgaaaac ttccgcgaat ccatgagtcg ggccatcgag caatgtatag
     2761 aacgctatga accccggatc tcacacgctg aagtgcaggc agtggtttca tccgcctcca
     2821 gcccgctgga tatgacgttt catattacag cctgggtgac atttaacgaa acgcatgagg
     2881 tgcttgaatt tgatatggca ccgaatggca gccagcatta ccgggtggac tgacaaatgg
     2941 aatttgaaga acgctatttt cgggaagaac tcgattacct gcgccagctt agcaagctgc
     3001 tggcaacgga aaaaccccat ctggcccgct tcctggccga aaaagatgcg gatccggata
     3061 ttgaacgcct gctggaaggg gtggcttttc ttaccggcaa tctccgccag aaaattgagg
     3121 atgaattccc agaactgacg cacgggctta ttaagatgct atggcctaat tacctgcgtc
     3181 cggttccggc aatgaccctt attgaatata cgccggatat ggataagtct tctgtaccgg
     3241 tgttaatccc ccgtaatgag cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag
     3301 tgctgccctc tgatgctaaa aaggaggagc cgcctccctg taccttcacc ctctgccggg
     3361 atatctggct gctgcccgtt cgcctggagc agattgaaaa ccgcagtacg acccgtaatg
     3421 gtgttatcaa catcaccttt tcggtcgcac cgggaacgga cttccgcacg ctggatctga
     3481 acaaacttcg cttctggctc ggcaatgacg acaactatac ccgtgaccag ctttatttat
     3541 ggttctgcga atacttgcag ggtgccgacc tgactgtggg tgaacagcat attcgcctgc
     3601 ctgagtttat gctaaaagct gtcggttttg agccgcagga tgccatgctg ccctggccga
     3661 aaaacgtcca cagcggctac cggatccttc aggagtattt ctgttacccc gatgcgtttc
     3721 tcttttttaa tctttgtggt tgtccggctt tgcctgacgg attgcaggcg gaattcttta
     3781 ccctgcaact gcgtttttcg cgccctttgc ccgtggacat ccggctgcgc cgcgattccc
     3841 tgcgcctgta ttgcgcacct gccattaatt tatttatcca ccatgcagaa gccatcacgc
     3901 tggacaaccg gcgggcagac tatccgctgg ttcccagccg ccattaccca caacattacg
     3961 atgtattttc cgttaacagt gtggtgagcc aggtccagga tatgttcagg aaaaaagatc
     4021 tggggcgtcc tgtttcgacg caggccgcgc gccagtggcc agcctttgaa agtttcagcc
     4081 atcagatgga atacagccgg aagcgggaag tggtgtactg gcatcaccgg accaaaacat
     4141 ccctgttcca tcgcggcttt gatcataccc ttgcctttat acatgctgat ggcagttatc
     4201 cgtcagacga atctctgctc agtaatgaag tggtttcggt atcgctgacc tgtaccaacc
     4261 gtgagcttcc gtcacaaatt cgttccggcg atatcaccgg cacaaccggt aaaaatgcgg
     4321 ctgttgcttc ctttcgcaac attacccgcc cgacgcaacc actctggccg gtcattgatg
     4381 gcagcctgca ctggtccctg ctctccgcca tgaacctgaa ttatctgtca ttactggata
     4441 cggacgcgct gaagcaggtc atcgccaact ttgatcgcca cgcaatccac catccgcaga
     4501 cggcgcggct gtcacaacaa aagctggatg ccattgagcg tctggagacc cgccccgttg
     4561 atcgcctgtt tacgggtatt cccgtccggg gactggcctc cacgctgtat ctgcacccgg
     4621 agccgtttgt ctgtgaaggg gaaatgtatc tgctcggtac ggtgctttcg cattttctgt
     4681 cgctgtacgc cagcgttaac tcattccaca tgctgaccgt tgtgaacaca gaaagccagg
     4741 agacatggaa atggacggaa agaatcgggc agcatcctct tatctgagtc cgggaaatcc
     4801 cccggcagat aaagagcaga atgatccgct ggcacaggtt ttccataatg cctgctctta
     4861 caattttttt gcgatggcgg agctgttgca ccgcctggca aagggtgaaa aggggacgcc
     4921 agaattatcc ctgcgcgacg atccggcaca ggaaaccctg cgtttcagcg ccgatgccag
     4981 ccttgccttc ccgtgcagtg atatcagtgc gctgaaacgg gatacgtcag gggcattcag
     5041 gatgaccacc acctttatgg ggttacaggg gagccagtcg cctctccccg gttattacct
     5101 cgatcacctg gcctggaaag cggtacatga acaaagtccg gtaggtgatt ttctggatat
     5161 gttcagccac cgcctgaccc agtttgtctg gcatatctgg cgtaagtacc gctatcacat
     5221 cagttttcgt aacggcggtg tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct
     5281 gggccaccgc cagcttcgcg ataagctggc gattaatcac agcaaaatgc tggcgtattc
     5341 cgggatcctg gcaaatccgg gacgatcccc ggaaattatc tgtggcctgg tgtcccactg
     5401 ctttgatttg tcagaagtga cgctgcaaaa ctggcaacgt cgcaaggtgg atattgaacc
     5461 ggatcagcaa aatagtctgg ggagctattc tcttaaaaat ggcgaaaaac tggcgggaag
     5521 atcggtactg ggcaactttg ttctgggtac acgagtgccc gatctcagcg gaaaattcca
     5581 gctcagtatt accagcctga cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa
     5641 cttcctgcca ctgacgatgt tcgtgtcctt catcctgcgc gatcagcttg cctgggactt
     5701 acatctgggg ctggcgccgg agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc
     5761 gctgctgggc tggaccagct tcctcggcac accggaagaa cgaccctcag tcaccatcag
     5821 ggtacggtcg taaattatga taatcacgga gcagaatatg ccggaagaga aactacagac
     5881 gctttcattg caggtcatta acggcagtga gctggaaagc gggcgggcgg cgcgctgtct
     5941 gttcacacag cagggaaatg tcggccatgg ccccgaatgc cactggtcgg tacaggatcg
     6001 tcagcagagc attccggccc aggcttttac cgttatcctg cacgatggca cattttgtct
     6061 gcgcccgcag acggcacaac tgtggctgaa tcaggcaaaa gtcacagcaa catcagacct
     6121 gatacagttg cgccagggcg atgagatcca gatcggacgg ctgatggtga gggttcatct
     6181 gaaccgggga gatattcccc attacgatga ggaaatggcc actcccgaaa ccatcgttac
     6241 caatcgcgat atgctcacgg ataccctgct atcaacggag ggtgcgccac actatccggg
     6301 aatgactcac cggcaccagc ttgcagacac cgtggtaaac ggtttttctg ccgatccact
     6361 ccaggcactt cagtccgaaa gcctgattac cacgggcgat ccgctttcag gcattgcggc
     6421 tgtccggcca tcggcaccgc tgtccgatcc ggcaagtaat ggggggatca atactccgtt
     6481 tatggatctg ccgcccattt atgccagccc cggcgatcgt aatgacgacg tctctgcggc
     6541 agaaatggcg caacgccacc ttgcggtcac ccccttactg cgcggtcttg gcggctcgct
     6601 taccatgagc aattccgacg atgcggatga ttttctggag gaggccggac gaacgttaca
     6661 ggccgcaata aaaggtctgc tcgatttgca gcagcagcgt aacagcctct cagacaaaca
     6721 tttgcgcccg ctggaagata acccactgcg cctgaacatg gattacgcca ccgcgctcga
     6781 cgtgatgttt gccgaaggta aaagcccggt acatctggcg gctcccgccg ccgtcagtga
     6841 aagcctgcgc aatgtccgcc accacgaaga agctaaccgg gcagcgattg tggagtcgct
     6901 tcgtgtcctg ctggatgctt tctcaccaca aaatctgctg cgccgctttg tgcagtaccg
     6961 ccgcagccat gaactgcgcc agccgctgga tgatgccgga gcatggcaaa tgtacagcca
     7021 ttattacgaa gaactggcct ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt
     7081 ctacgcccag gtctatgacc gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac
     7141 aggaaggcgt ttctggcctg cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg
     7201 aaaaaaatca gcgaggtgat caaaaacccg gatatccagg tcgggagcct gaaggagcaa
     7261 ccttcggaaa taaccgtcac cctgctgact gaaccggaca ccaataccaa tgcggagggt
     7321 gaatcagccg ccgtggatgt gcaactggtg tacctgactg acgactcaaa attgcaggca
     7381 gccgactatg accagatagc cagtacgccg ctaccggatg tgctgggtaa aaactatatc
     7441 gaccaccagg atttcaacct gttgccggac accatcaaaa ccctgccgcc gataaagctg
     7501 gatgagaaaa cgcagtttat tggtgtcgtg gcctatttct ccgatgacca ggccactgaa
     7561 tggaaacaaa tcgagacggt ggaaggaacc ggccaccatt accgcctgct ggtgcatgta
     7621 cgtcaaagca gcatcgaaat gaaaaaagag gatgaataat tatggcaacc acccgcaaca
     7681 aggtgatgtg gcaggaaggg atgctgatgc gcccacacca tttccagcaa cagcagcgtt
     7741 acaacgacta cctggataac cagcgtttcc gggccatgaa tgatttatcc tggggattta
     7801 ccgaactcac cctcaacaat gaactgctgg cgcagggtaa gatcatgatt gacagcgcgt
     7861 caggcacact gcctgacggc accgtctttt ctatccccga ccaggacgca ctgcccgatc
     7921 cgctgcaccc gcaaaatttt ccggacgaaa gaagccgcaa tatttacctc gctctgccgg
     7981 tcgccagtga tgtgagaaat gaaatcagcg acgggcggcg aatcgggcgt taccggctga
     8041 attatgccga tgtccgggat ctgcattcag aagaaggcga cgcgcgaaca ctgacgctgg
     8101 gacaactgac gccgcgcatt atgagcggtg cagaagatat gagtgcctat attacgctcc
     8161 cgctttgccg tatcagcgat cgccatgccg acggctccct gacgctggat gacgatttta
     8221 tcccctcctg ccagaatatt caggtcagta agaaactgcg tgtttatctc aaagaggtac
     8281 agggggccat tggcggacgg gcaagcgatc tggcaaaccg tattggctct ccggcgcaga
     8341 gcggcatcgc ggatgtggcg gaatttatga tgttgcagtt gcttaaccgt aaccagaccc
     8401 ggtttaccca tcgcgcccgc cgatcccagc tccacccgga agatttctac cttgatcttg
     8461 ccgggttgct gggtgaactg atgaccttta cagagccgtc acgcctgccc tgcccgcttg
     8521 atgtgtatga tcatcatgac ctgaccaaaa catttaaaac actgttaccg gaagtcaaac
     8581 gggcgctgca taccgtactg tcgccaagag cggtcaatct gccgctgcat ctgcgtgacg
     8641 gcatctggca ggccgatgtt catgactcgg aactgctaca gtctgccacc tttgtactgg
     8701 ccgtggcagc aaatatgcct gtcgatcaga tccagcgtca gtttatccag cagtcgaaaa
     8761 tttcctcgcc ggaaaaaatc cgcaatatgg tcagtgtgca gatcccgggt attcctctgc
     8821 gcgcactgat ggtggccccc cgccagcttc cctaccattc cgggttcagc tatttcgaac
     8881 tcgacaagag cggccaggcc tggacagaaa tggctgccgc cggggccgtt gcactgcatg
     8941 tatccggcag tttcccggat ctgaacatgc aactgtgggc gataagaggg taagacgatg
     9001 gatgaggggt cactgtctct gccaccattt accggatacg acgaaaaaag cctgcgcgac
     9061 taccatctcg cgcttcgtgg caacagcctg aatccgatga tcgacgcggc gacgccgcta
     9121 ctcggtatgg taatgcgcct ttccaccatg aacagccaga cgatgccgga gcatcttttt
     9181 gctcaggtgg tgacagatgt tcaggccgtg gagcaactgc ttcaggaaca gggctacgaa
     9241 ccgggcgtga ttatctcgtt tcgctacatc ctctgcacgt ttattgatga agcggcgctc
     9301 ggtaacggct ggtcgaacaa gaacgagtgg atcaagcagt cattgctggt ccatttccat
     9361 aacgaagcct ggggtggcga gaaagttttc atcctgctgg agcgcctgat ccgtgaaccc
     9421 aaacgttacc aggatctgct ggaatttctc tggctctgtt tttctctggg atttcgcggg
     9481 cgctacaaag tggcagtaca ggatcagggg gaatttgagc agatctaccg ccgtctgtac
     9541 cacgttctgc ataaactgcg cggcgatgcc cctttcccgc tgttacacca ggacaaaaaa
     9601 acccagggcg ggcgttatca gctcatcagt cgcctgactg ttaaacatat tttttgtggt
     9661 ggcgttgtcg tgctggcgtt gttttacctg ttctatctgt tgcggctgga cagccagacg
     9721 caggacatcc tgcaccagct aaacaaatta cttcgataag gacgtttatg atccagattg
     9781 atcttcccac gctggtaaaa cggctgaacc tgttctcccg ccaggcgctg gagatggccg
     9841 cctcagaatg tatgagccag caggcagcgg aaattaccgt cagccatgtg cttattcaga
     9901 tgctcgccat gccacgcagt gacctgcggg ttattacccg ccagggcgat attggcatgg
     9961 aagagttgcg ccaggcgttg acggtagaga actacacaac cgcccgttct gcggacagct
    10021 acccggcgtt ttccccgatg ctggttgagt ggcttaaaga gggctggctg ctggcgtcgg
    10081 ctgagatgca gcacagcgaa ctgcgcggcg gcgtgttgct gctggccttg ctgcattcgc
    10141 cgctgcgtta tataccgcct gctgccgccc ggctgttgac cggcattaac cgtgaccgtc
    10201 tgcaacagga ctttgtgcag tggacacagg agtcggcgga atcagtcgtg ccggatgcag
    10261 acggtaaagg cgcaggcaca ctgacggacg cctctgacac cctgcttgcc cgctacgcca
    10321 aaaacatgac agaagacgcc cgtaacggca ggctagaccc agtactgtgc cgcgaccacg
    10381 aaatcgacct gatgatcgac attctctgcc gccgccgtaa aaacaacccg gtggtggtgg
    10441 gcgaagcagg cgtgggtaaa agcgcgctga ttgaagggct ggcactgcgc atcgtggcag
    10501 gccaggtgcc ggacaaactg aaaaacaccg atatcatgac ccttgacctg ggcgcattgc
    10561 aggccggggc atcggtgaag ggtgagtttg aaaaacgctt caaggggctg atggcggagg
    10621 tcatttcctc tccggtgccg gtcattctgt ttatcgacga agcgcatacc ctgattggcg
    10681 cgggcaacca gcagggcggg ctggatatct ccaacctgct caaaccggcg ctggcgcgcg
    10741 gcgagctgaa aaccatcgcc gccaccacct ggagcgagta caaaaaatac ttcgagaaag
    10801 acgccgccct gtcgcgccgc ttccagttgg tgaaggtcag cgaacccagt gccgccgaag
    10861 ccaccattat tctgcgcggt ctgtcggcgg tctatgaaca gtctcacggc gtcctgattg
    10921 atgatgacgc cttgcaggcc gctgcgacat taagtgaacg ttatctctcc gggcgtcagt
    10981 tgccggacaa agcgattgat gtgctggata ccgcctgcgc ccgtgtggca atcaacctgt
    11041 catcgccacc gaaacaaatc tcggcgctga ccaccctgag ccaccagcag gaggcggaaa
    11101 ttcgccagct tgagcgcgag cttcgcatcg gactgcgtac cgacacatca cggatgaccg
    11161 aggtgctggt gcagtatgat gaaacgctga cggcgctgga tgaactggaa gcggcctggc
    11221 accagcagca gacgctggtc cgggagatta tcgcgctgcg ccagcagtta ctgggcgtgg
    11281 cagaggacga tgcggcgccg ttgccggacg cagataccgt ggaggatacg cagccagagt
    11341 cagaacagga taataccggt gctgtaccgg ctgatgaagc tggcagcgta cagccggaag
    11401 agaccgctga aacagtttcc ccggtacagc ggctggccca gctcactgcc gagctggacg
    11461 ccctgcataa cgaccggttg ctggtctccc cgcacgtcga taaaaaacag attgcggcgg
    11521 tgattgccga atggaccggc gtgccgctca accgcctgtc gcagaatgaa atgtcggtca
    11581 tcaccgacct gccgaaatgg ctgggcgaca ccatcaaagg ccaggacctg gcgattgcca
    11641 gcctgcataa gcatctactg accgcacgcg ccgacctgcg tcgtccggga cgcccgctcg
    11701 gtgcgttcct gctggctggc cccagcggtg tgggtaaaac cgaaaccgtc ctgcaactgg
    11761 cagagctgct ctacggcggt cgccagtacc tgaccaccat caatatgtcc gaattccagg
    11821 agaaacacac cgtctcgcgg ctgattggtt cgcctccggg ctacgttggc tacggcgaag
    11881 gcggcgtact gaccgaagcg attcgccaga aaccctactc ggtagtactg ctcgatgaag
    11941 tggaaaaagc gcacccggat gtgctcaacc tgttctacca ggcgttcgat aagggcgaaa
    12001 tggcagacgg tgaaggccgc ctgattgact gcaaaaatat cgtcttcttc ctgacgtcca
    12061 acctcggcta ccacgtaata gtcgagcatg ccgatgaccc ggaaaccatg caggaagtac
    12121 tgtatccggt gctggccgac ttcttcaaac ctgccctgct ggcgcgtatg gaagtggtgc
    12181 cgtatctgcc gctgtcgaaa gagacgctcg ccaccattat cgccgggaaa ctggcccgtc
    12241 tggataacgt gctgcgcagt cgctttggtg cagaagtggt cattgaaccg gaagtgacgg
    12301 acgaaatcat gagccgcgtc acccgcgcgg aaaacggcgc gaggatgctg gaatcggtca
    12361 tcgatggcga catgctaccg ccgctctcgc tgctgctgtt gcagaaaatg gcggctaaca
    12421 cggcgattgc ccggattcgg ttgtcggcag tggacggcgc atttacggca gacgtggaag
    12481 atgctcagaa cgacgagtcc gtcacaaagg atgaaacggt tttatgagca ggttgcgcgt
    12541 tttccggcag acaggatggt ttatcgcggg cctgatgacg ggcttacccg ccactgccgc
    12601 gcccgcagaa gcatcgtcaa tggccggtgt ggcggtcgcc gtcgccacca cgaccccgcc
    12661 ggatgcgaca gccacgttac aggccatgca gtcctgtcgg cgggagtccg cggcgctgga
    12721 gcggcttgac tgctatgacc gcctgctggc accgctgtcc ccgtcagggt ttgacggggc
    12781 gctggtcaaa gccggttttg tgggcgaggc ctggacccgc gccacagagc aggaaaaacg
    12841 ccgcgagggc aacaccacag aactgctggt gacgcaggtg ccgggggagc gcccgacggt
    12901 ggtgataacc accccggcta tcggccatgt tccgccgcgc ccggtgctga tgttcagttg
    12961 cgtggacaat atcacccgga tgcaggtggc gctgatgcac cctctggatg tccatgatat
    13021 cgccgtcact ctgaatgccg acagccgggc attgcgcagc cactggtttg tgcgcgagaa
    13081 cggcaccctg ctggagtcca gccgggggct gtccgggatt gatgaaatta agcaactgtt
    13141 cggcgcaaaa acgctgacag tggataccgg cgcagataac gccgcaggaa agctgacctt
    13201 taacattgac gggctggcaa gggcgattgc ccccctgcgc gatgcctgcc actgggcggg
    13261 agaataaaaa tatggcaatg gacttacgcg atccgaatgt ctggatatcg cacctgctgg
    13321 aaaacctgcc ggaagaaaaa ctggcatcgg cgctgaaaga tgacaacccg gactgggagt
    13381 atatcgacgg cgaaatcgtc aaactgggat ctctggccca cagtcaactg gatatcccgg
    13441 aactccagcg ccgggggctg gtcattctgg cttcggagag taaggacttt cgtctgctgg
    13501 cgcatctgct gagaaccctg caacacgccg gtgatcctca tctcgcattg cgcctgctgg
    13561 cgctgtatgt ggagcattac tggtcggtgg ctgcgccgca gaatatggca cataaaaaac
    13621 gctttgccag ccaggtcatt aaacgctttg aaacaggtat tgaaggcttt tcacagaacg
    13681 ccgccacagc gcagcgcgac gcgctgtcag gggaactggc gaaactggcg cagtgctggc
    13741 agtcacataa cgccccggaa ctggcactgg ctaccgatga tctttttgcc ctgtaccagc
    13801 gtgcgtttaa tcgtgcagca ccggctcctg tccccactcc ggcggcctcc ggtagttcac
    13861 cacagaccac cgccacgtct gaaagcggcg tgacgcaacc cagtgctccg gctccacaaa
    13921 tagccattga cagtcacgac gacaaagcct ggcgcgacac gctgttaaaa gtggcggcca
    13981 ttttatgtga acgccagccg gactcgccgc agggctatcg cctgcgccgc catgccctgt
    14041 ggcaatccat caccagtaca ccccaggcgg aaagcgatgg acgtacccca ttggctgcgg
    14101 tctctgccga tatggtggcg gattaccagt cccggcttgc cagcgcggat atggcgctgt
    14161 ggcagcaggt tgagaaaagc gtattgctgg ctccttactg gctggacggt cactgtcttt
    14221 ctgcacagac ggcactgcgt ctgggttaca aacaggtggc agacaccatc cgcgatgagg
    14281 tcatccgctt ccttgagcgt ctgccccagc ttaccgggct gctgtttaat gaccgcacac
    14341 cgtttctcag tgagcagacg aaacaatggc tggctgcttc gcccgacggc aaagttgcac
    14401 cggttgcgca aatcggtgag gaatcgcagg cagccagagc ctgttttgct gggcagggtc
    14461 tggaggcggc gctgcgatat ctggacatgc tacccgaagg cgatccccgc gatcagtttc
    14521 accgccagta ccttgccgca cagttgacgg aggaggcggg gctgatacag cttgcgcagc
    14581 aacagtaccg gatgttgttg atgataggga gtcagatgat ggtgtctgac tgggagccat
    14641 cattacttac gcagcttgaa caaaaattca cggcagaaca ataaaagaga acaggagttt
    14701 ttgtgttcag attaccaaca ccccgactac tcagcggact caaatcagcc ctacgcccgg
    14761 cgatgccccg gtttaaagtc tccgctttct ggctgctgat actggcgtgg atttttctgc
    14821 tggtatggat ctggtggaaa ggcccgacgt ggacgctgta tgaagaacag tggctcaaac
    14881 cactggcgaa ccgctggctg gcaacggcgg catgggggat tatcgccctg gtgtggctta
    14941 ccgtccgggt gatgaagcgc ctgcaacaac ttgaaaaaat gcaaaagcag cagcgcgagg
    15001 aggccattga tccgctcagt gtggagctga acgcccagca gcgttacctt gaccgctggc
    15061 tgctgcgcct gcaacgccat ctcgacaacc gccgtttcct gtggcagttg ccgtggtata
    15121 tggtcatcgg cccggcgggc agtggcaaaa cgacactgct gcgcgagggg tttccgtcag
    15181 acattattta tgccccggag ggcgcgcgcg gcacagaaca acgcctgtac cttacgcccc
    15241 atgtcggtaa acaggcggtt atctttgata tcgacggcac actctgcgct cccgctgatg
    15301 cggatatcct gcatcgccgt ctgtgggaac atgccctcgg ctggctgaaa gaaaagcgcg
    15361 cgcgccagcc gctgaacggg attattctga cactcgattt acccgatctg cttaccgcag
    15421 acaaacgccg ccgcgagcat ctgttacaga cgctgcgcag ccgcttgcag gatatacgcc
    15481 agcatcttca ctgccagtta ccggtttacg tggtactcac ccggcttgat ttattgcagg
    15541 gttttgccgc cctgttccag tcactgaaca gacaggatcg cgatgcgatt ctgggcgtca
    15601 cgttcacccg ccgcgcccat gaaaatgatg actggcgaac agagttgaat gctttctggc
    15661 agacatgggt ggatcgaatg aacctagcgt tgccggatct gatggtcgct cagactcaca
    15721 cccgcgcgtc tttatttagt ttttcccgcc agatgcaggg aagccgtgaa ccgctggtgt
    15781 cactgcttga gggtctgctt gatggcgaaa atatgaacgt gatgctgcgt ggtgtctatc
    15841 tcacctcttc gcttcagcgt ggacagatgg atgatatatt cacccagtct gccgcccgcc
    15901 agtaccggct gggcaataac ccactggcgt cctggcctct ggtggacacc gcgccttatt
    15961 tcacccgcag cttgttcccg caggcattac tcgcagagcc taatctggca acggagagcc
    16021 gcgcctggct gatgcgttcc cgtcgccgcc tgacggtttt ctctaccaca ggaggcgtgg
    16081 cggcactgct gctcatcacc ggctggcatc actattacaa cggtaactat cagtccggca
    16141 tcaccgtgct taagcaggcc aaagccttta tggacgtgcc gcctccgcag ggggaagatg
    16201 actacggcaa cctgcaactg ccgctgctga acccggtacg cgatgccaca ctggcctatg
    16261 gcgactgggg cgaccgcagc cgtctggccg atatgggact gtaccaggga cgacgtatcg
    16321 ggccttatgt ggaacagacc tatctgcaac tgctggagca acgttacctg ccctcgctgt
    16381 ttaacgggct ggttaaagcg atgaatgccg cgccgccgga gagtgaagaa aaactcgcgg
    16441 tgctgcgcgt gatgcgaatg ctggaggaca aaagcggacg taacaatgag gtggtgaagc
    16501 agtatatggc aaaacgctgg agcgaaaagt tccacggcca gcgcgatatc caggcacaac
    16561 tgatgtccca tcttgactac gcgctggctc atactgactg gcacgcagag cgtcaggcgg
    16621 gcgacggtga cgccatcagc cgctggacgc catatgacaa gcccgtggta tcagcacaga
    16681 aagaactgag caaactgcct gtctaccagc gggtttacca gagcctgaaa acgcgggcgc
    16741 tgggcgttct tcctgccgac ctcaatctgc gtgaccaggt agggccaacc tttgaccagg
    16801 tatttacatc tgccgatgac aacaaactgg ttgttccaca gtttcttacc cgttacggcc
    16861 tgcaaagcta ttttgtaaaa cagcgcgatg aactggttga actgacggcg atggattcct
    16921 gggtacttaa ccttacccgc agcgtgaaat acagtgacgc cgaccgcgcg gaaatccagc
    16981 gccagctcac cgagcagtat atcagcgact acaccgccac ctggcgggcc gggatggaca
    17041 atctgaatat ccgcaatttt gagtccatcg gacaactgac cggggcgctg gagcaggtta
    17101 tcagcggcga ccagcctttg cagcgggcgc tgaccgtgct gcgtgacaac acacaaccaa
    17161 gcgtcttttc tgaaaaactc tctgccaaag aacgggatga ggccctggca gagccggact
    17221 accagttact cacccgtctc gggcatgagt tcgccccgga aaacagtacc ctggcagtac
    17281 agaaagacaa agaaagcacg atgcaggcag tatatcagca actcaccgag ttgcaccgct
    17341 atctgctggc gatccagaac gcgcctgtac cagggaaatc ggcgctgaaa gccgtgcagt
    17401 tacggcttga tcagaacagc agcgatccga tattcgccac ccgccagatg gcaaaaacgc
    17461 tgcctgctcc gctcaaccgc tgggttggca ggctggctga ccaggcctgg catgtggtga
    17521 tggtggaggc cgttcactat atggaagtgg actggcgcga cagcgtggtg aaaccgttta
    17581 acgagcaact ggcaaataac tatccgttta atccgcgttc tgcacaggat gcctcactgg
    17641 atgccttcga acgcttcttt aaaccggatg gcatactgga taccttctac cagcagaacc
    17701 tgaagctgtt tatcgataat gacctgagtc tggaggatgg cgataacaac gtcattattc
    17761 gcgaagatat tattgcgcaa ctggaaactg cgcagaaaat ccgtgacatc ttcttcagca
    17821 aacagaacgg tctgggaaca tcctttgccg tggaaacggt atcgctttca ggcaataaac
    17881 gccgcagtgt actgaacctt gacggtcagt tagtcgatta cagccagggc cgtaactata
    17941 ccgcccatct ggtctggcct aacaacatgc gcgaaggcaa cgaaagtaag ctgacgctca
    18001 tcggcaccag cggcaacgcg ccgcgcagta tcagcttcag cgggccgtgg gcgcagttcc
    18061 gcctgttcgg ggccggacaa ctgaccggag tacaggatgg caactttacc gtgcgcttta
    18121 gcgtggacgg tggcgcgatg acctaccgtg tgcataccga cacggaagat aacccgttca
    18181 gcggtgggtt gttcagccag tttggtctgt cagacacact gtactgagga ggcagggatg
    18241 aatagtaacg tactgacaca aactatcgtt accggcagtg acccgcgcgg gctgccggaa
    18301 ttcagcgcca tccgcgagga aataaacaaa gccagccacc cgtcacagcc tgagctgaac
    18361 tggaaactgg tggagtcgct ggcgctggcg atttttaaag ccaacggtgt ggatttacac
    18421 accgccacct actatacgct tgcccggaca cggacacagg gactggcggg attctgcgaa
    18481 ggtgcggaac tgctggcggc aatggtaagc cacgactggg ataagttctg gccgcagggc
    18541 ggcccggcgc gtactgaaat gctggactgg tttaactccc gaaccggcaa tattctgcgt
    18601 cagcaaatct cctttgcgga atctgatctg ccgctgatct accgcacaga gcgggcattg
    18661 cagcttatct gcgacaagct tcagcaggta gagctgaagc gcgttccgcg cgtggagaat
    18721 ctgctctatt ttatgcagaa cacgcgtaaa cggcttgaac cccagctgaa gagtaacact
    18781 gagaacgccg cacagaccac ggtcagaacg ctgatttatg ccccggaaac acagccatct
    18841 tccacaccag aagcggtagt gcctcccctg cccggcctgc ctgagatgaa agtggaagtg
    18901 cgcagtctga cagagaatcc cccacaggcc agtgtgataa agcaaggcag tacggtaaga
    18961 gggtttatcg cagggatcgc ctgttcagtg gctgtcgcct cagcattgtg gtggtggcag
    19021 gtctatccgg tgcagcagca actgttacag gttaacgaca ccgctcaggg cgcagcaacg
    19081 gtgtggatgg cctcacctga actcgaaaac tatgagcgca ggctgcaaca acttcttgat
    19141 acctccccgg tacagccgct ggaaaccggg atgcagatga tgcgtgttgc caacagtcgc
    19201 tggccggaaa gcctgcaaca gcaacaggcc tcgacacaat ggaatgaggc actcaaaacc
    19261 cgcgcacaga atagcccgca gttgcgtggc tggttgcaga cccgccagga cttacatgct
    19321 tttgcagatc tagtgatgca gcgcgagaaa gagggactca ccctttccta tatcaaaaat
    19381 gtcatctggc aggcggagcg gggactgggg caggaaacac ccgttgagtc tctgttgacg
    19441 cagtaccagg atgcccgtgc gcagaagcag aatacagatg cgctggaaaa acaaattaat
    19501 gagcgactcg aaggcgtgtt aagccgctgg c
//
LOCUS       DAZUCE010000080        19432 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZUCE010000080 DAZUCE010000000
VERSION     DAZUCE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.080"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            171..1388
                     /gene="alaA"
                     /locus_tag="ACHY03_002399"
     CDS             171..1388
                     /gene="alaA"
                     /locus_tag="ACHY03_002399"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFS3905617.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1472..2071
                     /gene="yfbR"
                     /locus_tag="ACHY03_002400"
     CDS             1472..2071
                     /gene="yfbR"
                     /locus_tag="ACHY03_002400"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFS3905618.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLAARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2130..3962)
                     /gene="yfbS"
                     /locus_tag="ACHY03_002401"
     CDS             complement(2130..3962)
                     /gene="yfbS"
                     /locus_tag="ACHY03_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFS3905619.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4049..4699)
                     /gene="hxpA"
                     /locus_tag="ACHY03_002402"
     CDS             complement(4049..4699)
                     /gene="hxpA"
                     /locus_tag="ACHY03_002402"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFS3905620.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4710..5204)
                     /gene="yfbU"
                     /locus_tag="ACHY03_002403"
     CDS             complement(4710..5204)
                     /gene="yfbU"
                     /locus_tag="ACHY03_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFS3905621.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5286..5741)
                     /gene="yfbV"
                     /locus_tag="ACHY03_002404"
     CDS             complement(5286..5741)
                     /gene="yfbV"
                     /locus_tag="ACHY03_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFS3905622.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6079..7281
                     /gene="ackA"
                     /locus_tag="ACHY03_002405"
     CDS             6079..7281
                     /gene="ackA"
                     /locus_tag="ACHY03_002405"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFS3905623.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7356..9500
                     /gene="pta"
                     /locus_tag="ACHY03_002406"
     CDS             7356..9500
                     /gene="pta"
                     /locus_tag="ACHY03_002406"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS3905624.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9690..11210
                     /gene="yfcC"
                     /locus_tag="ACHY03_002407"
     CDS             9690..11210
                     /gene="yfcC"
                     /locus_tag="ACHY03_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFS3905625.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11243..11785)
                     /gene="yfcD"
                     /locus_tag="ACHY03_002408"
     CDS             complement(11243..11785)
                     /gene="yfcD"
                     /locus_tag="ACHY03_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFS3905626.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11843..12394)
                     /gene="yfcE"
                     /locus_tag="ACHY03_002409"
     CDS             complement(11843..12394)
                     /gene="yfcE"
                     /locus_tag="ACHY03_002409"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFS3905627.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12450..13094)
                     /gene="yfcF"
                     /locus_tag="ACHY03_002410"
     CDS             complement(12450..13094)
                     /gene="yfcF"
                     /locus_tag="ACHY03_002410"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFS3905628.1"
                     /translation="MSKPAITLWSDAHCFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPECLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13230..13877
                     /gene="yfcG"
                     /locus_tag="ACHY03_002411"
     CDS             13230..13877
                     /gene="yfcG"
                     /locus_tag="ACHY03_002411"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFS3905629.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13934..14296
                     /gene="folX"
                     /locus_tag="ACHY03_002412"
     CDS             13934..14296
                     /gene="folX"
                     /locus_tag="ACHY03_002412"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFS3905630.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14317..15210
                     /gene="yfcH"
                     /locus_tag="ACHY03_002413"
     CDS             14317..15210
                     /gene="yfcH"
                     /locus_tag="ACHY03_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFS3905631.1"
                     /translation="MNIVITGGTGLIGLHLIPRLLELGHQITVMTRNPQKASSVLGPQ
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15258..16160)
                     /gene="rpnB"
                     /locus_tag="ACHY03_002414"
     CDS             complement(15258..16160)
                     /gene="rpnB"
                     /locus_tag="ACHY03_002414"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFS3905632.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16357..17130)
                     /gene="hisP"
                     /locus_tag="ACHY03_002415"
     CDS             complement(16357..17130)
                     /gene="hisP"
                     /locus_tag="ACHY03_002415"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFS3905633.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17138..17854)
                     /gene="hisM"
                     /locus_tag="ACHY03_002416"
     CDS             complement(17138..17854)
                     /gene="hisM"
                     /locus_tag="ACHY03_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3905634.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17851..18537)
                     /gene="hisQ"
                     /locus_tag="ACHY03_002417"
     CDS             complement(17851..18537)
                     /gene="hisQ"
                     /locus_tag="ACHY03_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFS3905635.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFLRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18627..19409)
                     /gene="hisJ"
                     /locus_tag="ACHY03_002418"
     CDS             complement(18627..19409)
                     /gene="hisJ"
                     /locus_tag="ACHY03_002418"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFS3905636.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKCFDFDVYGG"
BASE COUNT         4854 a         5183 c         4755 g         4640 t
ORIGIN      
        1 atacacctca ttattttgtc attattaagt tattaacagc acaatcgaac cttcccctct
       61 ggcaaaatct tattctgcag accttcaaaa caccgtcctg ggggagtaca ttgttctaag
      121 ctgacttcca cggcagggag tggcgataac agcaaaaaag gtcaagattc atgtccccca
      181 ttgaaaaatc cagcaaatta gagaatgtct gttatgacat ccgtggtccg gtgctgaaag
      241 aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa actgaacatc ggcaacccag
      301 ccccgttcgg ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc aacctgccta
      361 cagctcaagg gtattgcgat tccaaaggtc tttactccgc gcgtaaagcc atcatgcagc
      421 actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc ggcaatggtg
      481 tatcggagct tatcgttcag gcaatgcagg cattgctgaa cagcggggac gaaatgttgg
      541 ttcctgcacc agattaccca ctatggaccg cggcggtttc gctttccagc ggtaaagcgg
      601 tgcattatct ttgcgatgaa tcctctgact ggttcccgga cctcgatgat attcgcgcta
      661 aaattacgcc tcgtacgcgt gggatcgtta ttatcaaccc aaataaccca accggcgcgg
      721 tatattccaa agagctttta atggagattg tggagattgc acgtcagcat aatctcatta
      781 tcttcgccga tgaaatttat gacaaaattc tctacgacga cgctgagcat cactcaattg
      841 cgccgctggc acctgacctg ctgaccatta cctttaatgg actgtcgaaa acgtaccgcg
      901 ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac gccaaaggct
      961 acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt cctgcgcaac
     1021 acgccattca gaccgcgctg ggtggttatc agagcatcag tgaatttatt acccctggcg
     1081 gtcgtcttta tgagcagcgt aaccgcgcgt gggaattgat caacgatatt ccgggcgttt
     1141 cctgcgtgaa acctcgtggt gcgctgtaca tgttcccgaa aatcgacgcc aaacgcttta
     1201 acattcacga cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa gttctgttgg
     1261 tgcaagggac ggcattcaac tggccgtggc cggatcactt ccgcattgtc acgctaccgc
     1321 gtgtcgatga tatcgagctg tctttgagca agttcgcgcg tttcctttct ggttatcatc
     1381 agctgtaatc ttaatttcac tgccggagat tgcatccggc agtgttatcc cgccacaatg
     1441 acctgatgat gtcatcatac gtaaggtcac tatgaaacag agccattttt ttgcccatct
     1501 ctcccgcctg aaactcatta accgctggcc gctcatgcgc aatgtgcgga cggaaaatgt
     1561 gtccgaacac agtttgcagg tagcgatggt cgcccatgcg ctggcagcta tcaaaaatcg
     1621 gaaatttggc ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt accacgatgc
     1681 ctcagaagtg ctcaccggcg atctccctac tccggtgaaa tacttcaatt cgcaaatcgc
     1741 tcaggaatac aaggctattg aaaaaatcgc tcagcaaaaa ctggtcgata tggttccgga
     1801 agagctgcag gatatctttg cgccgttaat tgacgagcat gcatatagcg atgaagaaaa
     1861 atcgttggtg aaacaggcag atgcactgtg tgcatatctg aaatgtctgg aagaactcgc
     1921 ggccggaaat aatgaattct tgctggcaaa aacgcgactg gaagcgacgc ttgcagcgcg
     1981 tcgcagccag gagatggact acttcatgga agtatttgtt cccagcttcc atctttcgct
     2041 cgatgagatt agccaggatt caccgctgta agcagccgga gtctgcgtcg catcaggcaa
     2101 taagcgccgg atgcgacatc aggctcttgt caaaacggaa acagcatcgg gatcatcacc
     2161 acacaaaccg ccatcacgat aatggtgaac ggtaccccca acttcacaaa gtcactgaag
     2221 ctgtaatttc ccggacctaa aaccagtgtg ttaacaggtg aagaaaccgg tgtcataaag
     2281 gcggcggatg ctgccatcgc cacgaccatc gcgaatggat aaggcgacac acccatcgtt
     2341 ttggcagcag ccagcgcaat cggagccatc aacaccgccg tcgcggtatt agagataaat
     2401 agcccaataa ccgccgacaa gacaaacaaa caacccagca tcatatgtgg cccgtaacca
     2461 ccgccaatgt ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt ctgtaatgcc
     2521 acagcaaacg gcatcatccc aacgatcaaa ataatgctcg gccagtgaat ggatttatag
     2581 gcgctttcag catctataca gcggaatttc cccatcagca ggcaggcgat gatagcggca
     2641 acaggattag gaatttcatc tgtcagcatt aacgccacca ttagcaccag acagaaaatg
     2701 gcatggggtg cctggctgtg cgcgggcgat gcttcactca cctcttccgg taagttcagc
     2761 gctacgaagt cgcggccctg tttggccagc ataccgatca gtttccagtt acccacaacc
     2821 aggatgatat cgcccagcag cagaggctca tccgccagcg aaccttccag cgccacgcca
     2881 ttgcgcttta gccccaccac attcagtccg tagcgggtac gaaaaccaat ttcgcgcacc
     2941 gatttaccaa tcagttctga ctcaggaatt aatgaaatct ctgccatgcc cacatcaagg
     3001 gcctggtcag aaaaatactc gccgcgcagt accatcggct ccagcaattg ctcactacaa
     3061 aattgccgga gatcgacatc agccgcagac atatcaataa gcaaaacgtc acgcgcgcga
     3121 aattcagaaa ccccattaac gttcacgata acgcgacgaa aacgccgcca gcgttcaaca
     3181 ccgatgacgt tagcgccata acgctcacgt aatttgagat cgtccagccg ttgaccaatc
     3241 attggcgatc cggggcggat agccagacgt cgcgcacgcc cggtcagtcg atattcacgg
     3301 ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg ggtatcccct
     3361 ttcagcatga aacgcatcac taacatatac aaaataccca gcaccagcac gaccaggcca
     3421 ataggtgtta cgctaaagaa actgaagccg tgatagcctt cacgcagcaa ttcactgttg
     3481 actaccaggt tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag cccggcaaaa
     3541 cttaacggca tcatcagacg tgacggcgaa gtttgcatac gcatggcaac gcttaacacc
     3601 acgggaataa agatagcgac aacgccggtt gaactcataa acgcgccaag ccccgcgacg
     3661 gtcagcatca acaaaaccaa cattttgatt tcactgttgc ccgcaacttt gaccagccat
     3721 gttcccatta cggtggcaac accggtacgg accaaaccat cgccaataat aaacaaggcg
     3781 gcaatcagga caacgttagg atcagaaaag ccggaaaata cttctgggac tgtcagcgtt
     3841 ccgcttaatg caaacgcgac aataacaaac aaagcgaccg catccatacg cactctgccc
     3901 gtcgcaaaca agacgatggc aacggccagt aatgaaagaa cccaaatcaa ttcaccgttc
     3961 acaacttatc cttgttaatt gagggggatg acttgattct gccataaaaa agccccgacg
     4021 agacggggct aaatcatgat caggtgtttc actgaataat aacatcgcca tttggctgtt
     4081 tggtcacagt aatttgctcc agactgtgga ggaccaaatc gacctcattc aggcgcgggg
     4141 tatctgccgg agcgttaacc gcaatgacat ggcaacccgc cgccaggcca gaaagcacgc
     4201 cagcgggagc atcttccacc accacacact cctgcggcgc aagccccagc agctgcgcgc
     4261 ctaacagata cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg gttacaaaca
     4321 cctctggtgc gggaagcccc gctattttat ggcgcgctcg cgctaccggc atggagccag
     4381 aagtcacaat ggcccacgga atacctgctt tattcaaatg attgagtaag gcgattgccc
     4441 ccggaagcgc ggtaatacct tcggtttccg tggcctcgat ttgctccaga cgcgtaaact
     4501 cggcggcaat atcagcctcg gatttgcccg ccataaaatg gcgcagagag gtgatcgcct
     4561 gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa cccatgacgt ctggcccagt
     4621 tgctccacgc ccgttctacc gcaggcaggg aatccaccag cgttccatca agatcaaaca
     4681 gaaaaccttt gcaccgcacg cgggcctcct caggcgttga taatttgatt aatttcgttg
     4741 gcgctcaaat ggtactgacg cgggcaggca tgccacacat taagcatgcg ctgatatttt
     4801 tcccacattg gcgtctgggc gttaaaaccg tgagttccgg cgtcaaaatg ggtatagcgc
     4861 ccttccacat taaccataaa gcggacataa ccgaggtaac gtgcttcagt ggcggcgtca
     4921 aagccaagga aggtgacacg acgttcatcg atggattgct ggtcctgcaa attagaccag
     4981 gaaacatgca acgcatgata catctccata atgtcgatga tagtgcggca ggtttcttct
     5041 ttcagctcgc caaactcgcg atccagttca cgcatctgta atccataacc acgctcaatg
     5101 attgtttgca ggcgacggta acgttcagca tttgccggat cgagcatagt catcatcttg
     5161 tactgattag acaaaataag acgttgcgcg ttggtcattt ccattgttga ctcctgtatc
     5221 actctattac ggtgaaaaaa agaaggctga gtatgccttc ttttatatgc gtaatcaggg
     5281 gtcaattaca aatcatcaag gaaagtttta tccagttgtt tgaaggcgcg cttaagcgtg
     5341 tcagctaatg cctggtaatc aggcttgcct tcaacgggtg ccaacacctg tccagactcc
     5401 tgcaatttac cgcgaacttc ataaaaccag ttgaggatag cagggggtaa tggcgttaca
     5461 gaacgcttgc ccagccacca caatccctgc atgggtaaac ttaaggcgaa cagcgcagtg
     5521 gcaactgccg gcccaagctg accgcccagg gcaatctgcc agcagagagt aaatacggcg
     5581 atcggcggca taaaacggat cgcataacgc gtcatcttga taacgcgatt ttcgacaaag
     5641 accggggcaa ggcgtttttc cagcggccac gtctttgagt aatgctgtcc ccggcgaaac
     5701 aagctaaaaa aattaacaga acgattatcc ggcgttgaca tgcttcacct caacttcaca
     5761 tataaagatt caaaaatttg tgcaaattca caactcagcg ggacaacgtt caaaacattt
     5821 tgtcttccat acccactatc aggtatcctt tagcagcctg aaggcctaag tagtacatat
     5881 tcattgagtc gtcaaattca tatacattat gccattggct gaaaattacg caaaatggca
     5941 tagactcaag atatttcttc catcatgcaa aaaaaatttg cagtgcatga tgttaatcat
     6001 aaatgtcggt gtcatcatgc gctacgctct atggctccct gacgtttttt tagccacgta
     6061 tcaattatag gtacttccat gtcgagtaag ttagtactgg ttctgaactg cggtagttct
     6121 tcactgaaat ttgccatcat cgatgcagta aatggtgaag agtacctttc tggtttagcc
     6181 gaatgtttcc acctgcccga agcacgtatc aaatggaaaa tggacggcaa taaacaggaa
     6241 gcggctttag gtgcaggcgc cgctcacagc gaagcgctca actttatcgt taatactatt
     6301 ctggcacaaa aaccagaact gtctgcgcag ctgactgcta tcggtcaccg tatcgtacac
     6361 ggcggcgaaa agtataccag ctccgtagtg atcgatgagt ctgttattca gggtatcaaa
     6421 gatgcagctt cttttgcacc gctgcacaac ccggctcacc tgatcggtat cgaagaagct
     6481 ctgaaatctt tcccacagct gaaagacaaa aacgttgctg tatttgacac cgcgttccac
     6541 cagactatgc cggaagagtc ttacctctac gccctgccgt acaacctgta caaagagcac
     6601 ggcatccgtc gttacggcgc gcacggcacc agccacttct atgtaaccca ggaagcggca
     6661 aaaatgctga acaaaccggt agaagaactg aacatcatca cctgccacct gggcaacggt
     6721 ggttccgttt ctgctatccg caacggtaaa tgcgttgaca cctctatggg cctgaccccg
     6781 ctggaaggtc tggtcatggg tacccgttct ggtgatatcg atccggcgat catcttccac
     6841 ctgcacgaca ccctgggcat gagcgttgac gcaatcaaca aactgctgac caaagagtct
     6901 ggcctgctgg gtctgaccga agtgaccagc gactgccgct atgttgaaga caactacgcg
     6961 acgaaagaag acgcgaagcg cgcaatggac gtttactgcc accgcctggc gaaatacatc
     7021 ggtgcctaca ctgcgctgat ggatggtcgt ctggacgctg ttgtattcac cggtggtatc
     7081 ggtgaaaatg ccgcgatggt tcgtgaactg tctctgggca aactgggcgt gctgggcttt
     7141 gaagttgatc atgaacgcaa cctggctgca cgtttcggca aatctggttt catcaacaaa
     7201 gaaggtaccc gtcctgcggt ggttatccca accaacgaag aactggttat cgcgcaagac
     7261 gcgagccgcc tgactgcctg atttcacacc gccagctcag ctggcggtgc tgttttgtaa
     7321 cccgccaaat cggcggtaac gaaagaggat aaaccgtgtc ccgtattatt atgctgatcc
     7381 ctaccggaac cagcgtcggt ctgaccagcg tcagccttgg cgtgatccgt gcaatggaac
     7441 gcaaaggcgt tcgtctgagc gttttcaaac ctatcgctca gccgcgtacc ggtggcgatg
     7501 cgcccgatca gactacgact atcgtgcgtg cgaactcttc caccacgacg gccgctgaac
     7561 cgctgaaaat gagctacgtt gaaggtctgc tttccagcaa tcagaaagat gtgctgatgg
     7621 aagagatcgt cgcaaactac cacgctaaca ccaaagacgc tgaagtcgtt ctggttgaag
     7681 gtctggtccc gacacgtaag caccagtttg cccagtctct gaactacgaa atcgctaaaa
     7741 cgctgaatgc ggaaatcgtc ttcgttatgt ctcagggcac cgacacccca gaacagctga
     7801 aagagcgtat cgaactgacc cgcaacagct tcggcggtgc caaaaacacc aacatcaccg
     7861 gcgttatcgt taacaaactg aacgcaccgg ttgatgaaca gggtcgtact cgcccggatc
     7921 tgtccgagat tttcgacgac tcttccaaag ctaaagtaaa caatgttgat ccggcgaagc
     7981 tgcaagaatc cagcccgctg ccggttctcg gcgctgtgcc gtggagcttt gacctgatcg
     8041 cgactcgtgc gatcgatatg gctcgccacc tgaatgcgac catcatcaac gaaggcgaca
     8101 tcaatactcg ccgcgttaaa tccgtcactt tctgcgcacg cagcattccg cacatgctgg
     8161 agcacttccg tgccggttct ctgctggtga cttccgcaga ccgtcctgac gtgctggttg
     8221 ccgcttgcct ggctgccatg aacggcgtag aaatcggtgc cctgctgctg actggcggct
     8281 acgaaatgga cgcgcgcatt tctaaactgt gcgaacgtgc tttcgctacc ggcctgccgg
     8341 tatttatggt gaacaccaac acctggcaga cttctctgag cctgcagagc ttcaacctgg
     8401 aagttccagt tgacgatcac gagcgtatcg agaaagttca ggaatacgtt gctaactaca
     8461 tcaacgctga ctggatcgat tctctgactg ccacttctga gcgcagccgt cgtctgtctc
     8521 cgccagcgtt ccgttatcag ctgactgaac ttgcgcgcaa agcgggcaaa cgtatcgtac
     8581 tgccggaagg tgacgaaccg cgtaccgtta aagcagccgc tatctgtgct gaacgtggta
     8641 tcgcaacttg cgtactgctg ggtaatccgg cagagatcaa ccgtgttgca gcgtctcagg
     8701 gtgtagaact gggtgcaggc attgaaatcg ttgatccaga agtggttcgc gaaagctatg
     8761 ttggtcgtct ggtcgagctg cgtaagaaca aaggcatgac cgaaaccgtt gcccgcgaac
     8821 agctggaaga caacgtggtg ctcggtacgc tgatgctgga acaggatgaa gttgatggtc
     8881 tggtttccgg tgctgttcac actaccgcaa acaccatccg tccgccgctg cagctgatca
     8941 aaactgcacc gggcagctcc ctggtatctt ctgtgttctt catgctgctg ccggaacagg
     9001 tttacgttta cggtgactgt gcgatcaacc cggatccgac cgcagaacag ctggcagaaa
     9061 tcgcgattca gtccgctgat tccgctgcag ccttcggtat cgaaccgcgc gttgctatgc
     9121 tctcctactc caccggtact tctggtgcag gtagcgacgt agaaaaagtt cgcgaagcaa
     9181 ctcgtctggc gcaggaaaaa cgtcctgacc tgatgatcga cggtccgctg cagtacgacg
     9241 ctgcggtaat ggctgacgtt gcgaaatcta aagcgccgaa ctctccggtt gcaggtcgcg
     9301 ctaccgtgtt catcttcccg gatctgaaca ccggtaacac cacctacaaa gcggtacagc
     9361 gttctgccga cctgatctcc atcgggccga tgctgcaggg tatgcgcaag ccggttaacg
     9421 acctgtcccg tggcgcactg gttgacgata tcgtctacac catcgcgctg actgcgattc
     9481 agtctgcaca gcagcagtaa tctcgtcatc atccgcagct ttgcgctgcg gatatctgaa
     9541 ccggaaatag tcactatttc cggtttttta ttctcttaat ttgcattaat cctttctgat
     9601 tatcttgctt aactgcgctg catcaatgaa ttgcgccatt tcactttgca tacttaccac
     9661 tttgttttgt gcaagggaat atttgcgcta tgtccgcaat cactgaatcc aaaccaacaa
     9721 gaagatgggc aatgcccgat acgttggtga ttatcttttt tgttgctatt ttaaccagcc
     9781 ttgccacctg ggtagttccg gtggggatgt ttgacagtca ggaagtgcag tatcaggttg
     9841 atggtcaaac aaaaacacgc aaagtcgtag atccacactc atttcgcatt ctgactaacg
     9901 aagcaggcga acctgagtat caccgcgtac agctgttcac gacgggcgat gaacgcccgg
     9961 gcctgatgaa cttcccgttt gaagggttaa cctcaggatc gaaatacggg acagccgttg
    10021 gcatcatcat gtttatgctg gtgattggcg gcgcgtttgg cattgtgatg cgtacaggaa
    10081 ccattgataa cggtatcctg gcgcttattc gccatacccg cgggaatgaa attctcttta
    10141 ttcctgcgct gtttattctg ttttcacttg gcggcgcggt atttggtatg ggagaagagg
    10201 ccgtcgcctt tgccattatc atcgcaccgc taatggtccg gctgggctat gacagtatta
    10261 ccaccgtcct ggtgacctat attgccacgc aaatcggttt tgccagttcg tggatgaacc
    10321 cgttttgtgt ggtcgttgct caggggattg ccggcgttcc ggtgctttct ggctccgggt
    10381 tgcgcatcgt ggtgtgggtt atcgccactc tgattggcct gatctttacc atggtgtacg
    10441 cctcacgagt gaaaaagaat cctcttctgt cacgcgtgca tgagtccgac cgcttctttc
    10501 gtgaaaagca ggcggatgtt gaacaacgtc cgtttacctt tggtgactgg ctggtattga
    10561 ttgtcctgac cgccgtaatg gtctgggtga tttggggcgt gatcgttaat gcctggttta
    10621 ttccagaaat tgccagccag ttcttcacca tgggtctggt gattggcatc atcggcgttg
    10681 ttttccgcct taacggcatg acggttaata ccatggcttc atcctttacc gaaggggcga
    10741 gaatgatgat cgcccctgcc ctgctggtgg gtttcgccaa agggattttg ctgctggttg
    10801 gtaatggtga agcaggtgat gccagcgtgt taaatactat cctcaacagc attgccaatg
    10861 ccattagcgg tctggacaac gcggtcgcgg cctggtttat gttgctcttc caggcggtat
    10921 ttaatttctt cgtgacgtcc ggttctggtc aggcggcgtt aaccatgccg ttactggcac
    10981 cgcttggcga tctggtcggt gttaaccgtc aggttaccgt gctggcgttc cagtttggtg
    11041 atggcttcag tcacatcatt tacccaacct cagcttcgtt aatggcaacg ctcggtgttt
    11101 gccgggtgga cttccgtaac tggctgaagg tgggcgcgac cctacttgga ctgctgttta
    11161 ttatgtccag cgtcgtggtg atcggcgctc agttgatggg ctaccactaa aaatgttaag
    11221 agccgcaatt gcggctcttt tttcattctg ccgtttcagt ctctacggct tcatttttgg
    11281 cattgcgctt catccacaac gccagcgctt tcagcgagtc tggagtgaac tcatcgcagc
    11341 gtgcggtgat ttcttccggc gtcagccagc aaacttcact gacttcatct tcctgtaggg
    11401 cgaagggacc gtgagagacg cagctgaaca atgcgcccca gacacggcaa tttttatctt
    11461 cgaaatagaa ctgcccgtgc tcggcaaagg ggacaccggc aatgcccaac tcttcttccg
    11521 cttcgcgacg cgcggattcc agcagttgct catcggcctg aaccacaccg cctgcggtcg
    11581 catctaacat gccgggtaaa aagtcttttg tctcggtacg acgctggacc agaattttgc
    11641 ccatgccatc atgcacgacg atgtaagttg cacgatgacg cagacactgt gcccgcattt
    11701 gttcccggct ggcttgtgca atgacttcgt tctcttcatt gacaatatcc acccattcag
    11761 tacttgccaa acgacgctgt tccattatca ggaaaccttc tttttctggc gctcttacgg
    11821 cgcattatga gttgtgggta aattacggat taatcgcgac ctgcgcaatg atactttgat
    11881 cattgagtgc gattacacta agtacgtcat tatccagcat gccataactc gccggattac
    11941 cgcctttcgg aatactcacc gagccggggt tgaagtggaa aatctccccc cgctgttctg
    12001 ccactggcag atgggtatgg ccgtacacca gcacatcgtt ctggtttaaa gcaggcagat
    12061 tttccgggcc aaaaagatga ccatgcgtca aaaacagacg ttgtttttcc agtaatacct
    12121 gttgccacgg cgcggttatc gggaaatgca gcagcatttg atccacttcg ctgtcgcagt
    12181 tgccgcgcac agcgataacc ttatgtgcca cttcattaag ccgttcagcg actttggctg
    12241 gcgcgtaacc ctccggtaaa gcattacgcg ggccgtgatt caacacgtcg ccaaggatca
    12301 ccagccactg ggcaccgctt tgggcaaaca actccagaac acgttccgtc gccggtaacg
    12361 acccatgaat gtccgatgca aacatcagtt tcatcactca ctcctcgtcg aaaaaacgta
    12421 agctatgata ctggattctg tcgttgctat cagccagatt gcttcgccga aagtgcaata
    12481 aaacgctgga cagacgctcg ctgccactgg aatgtcgcat aatccaccag gcattctggc
    12541 acctcatcgc catgtagtac caggcggtta atcattagcg ccagatcagt atcagcaatg
    12601 caccattcac caaataaatt cggctgaccc agtactaaca aatgttctgc catcgcgaac
    12661 agtttctctg cactggcttt tccctcggcc gttagtggcg cttttttcgc ccccgcaaag
    12721 acaacatccg tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga
    12781 atctgtcgtg cacgtgcacg attttctaaa tcaagcggat aaatacgttc ccaggtcggt
    12841 ggcgcaaatc gatcttccag atactccgca atggcagaag attcactcaa ctcaaaatca
    12901 tcgatttgta ataacggcac acggcgtgtc tgaccgtaac cttgccacgt cggttgcaaa
    12961 tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg acaggccttt ttcctgcaac
    13021 gccacccagg cggataacac ataaggggag aaacagtggg catctgacca aagcgtgata
    13081 gcgggtttac tcattatgtc ctcggcaata aagggggctg gctatcaaac tatagagcct
    13141 ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc gtgctttcta tacttgtggt
    13201 gttcagatga ttagacaacc ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca
    13261 atggccacaa aattacgctg tttctcgaag aagcagggct ggattatcgc ttgataaagg
    13321 tagacctggg gaaaggcggt cagtttcgcc cggaattttt gcgcatttcg cctaacaaca
    13381 aaattccggc aattgttgat cattctccag ccgatggcgg cgaaccgcta agcctctttg
    13441 aatctggtgc cattttgttg tatctggctg agaaaacggg gctctttttg agtcatgaaa
    13501 cgcgtgaacg cgccgccaca ttacagtggt tattctggca ggtaggcgga ctggggccga
    13561 tgcttgggca aaatcatcat tttaatcacg cagcccccca aaccattcct tacgctattg
    13621 aacgttatca ggttgaaact cagcgtctgt accatgtact gaacaagcgg ctggaaaact
    13681 cgccctggct gggaggcgag aactacagca ttgcggatat tgcctgctgg ccgtgggtta
    13741 atgcctggac tcgccagcga attgacctcg caatgtatcc ggcagtcaag aactggcatg
    13801 agcggatccg ttcgcgccct gccaccgggc aggcactgct aaaagcacaa ctcggtgatg
    13861 agcgttcgga tagttaacag aaacaggttc tcgtgtatta tttcatccta agtaaaacaa
    13921 cggagaacct gcaatggcac aacctgccgc tattattcgt ataaagaacc ttcgtttacg
    13981 tacatttatc ggaattaagg aagaagaaat taacaaccgt caggatattg ttatcaatgt
    14041 gacgatccac taccccgccg ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa
    14101 ttatcgcacc gtaacgaaaa acattattca gcatgtagag aataaccgtt tctctttgct
    14161 ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa catcactggg tgacgtatgc
    14221 tgaagtggag atcgataaac tgcacgcgct gcgctacgcc gattcggtat ccatgacctt
    14281 aagctggcag cgttaaccgc catatcggga ggctgcatga acatagtgat caccggaggg
    14341 acgggattaa ttggtctcca tttgattcca cgtttgctgg agctgggcca tcaaatcacg
    14401 gtaatgacgc gtaacccgca gaaagccagt tccgtgctcg ggccgcaggt gacgctatgg
    14461 caagggcttg ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa cctcgccgga
    14521 gaaccgattg ctgataaacg ctggactcac gagcaaaaag agcgtctctg ccaaagccgc
    14581 tggaatatca cgcaaaaact ggtcgatttg attaatgcca gcgacacgcc accgtcggta
    14641 ctcatttccg gctcggcaac gggctattat ggcgacttag gtgaagtggt ggttaccgaa
    14701 gaggaaccgc cgcataacga atttacccat aaactctgcg cccgctggga agaaattgcc
    14761 tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc gtaccggtgt agtgctggca
    14821 ccggatggcg gtattctcgg taaaatgctg ccgccgtttc gtcttggcct gggcggaccg
    14881 attggctctg gtcggcagta tctggcctgg attcatattg atgatatggt caacggtatt
    14941 ctctggctgc tggataacga gctgcgcggg ccatttaata tggtttcgcc ctacccggta
    15001 cgcaatgaac aatttgccca tgcgctcggt catgcgctgc atcgcccggc cattttgcgc
    15061 gtccccgcga ccgccattcg actgttaatg ggcgaatctt cagtactggt attaggtgga
    15121 caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg gtacgattta
    15181 gaagaggcgc tggcggatgt cgttcgctga tgtggtttac tgcaaacatc cgccagttaa
    15241 ctcccggtgt tacagggtta gtggctttgc gcgataagat cgtctggtga aagtcgggtc
    15301 accatcataa ctaactctcg gtctaaacct ctatccagca tctcctgagc aatacgcagg
    15361 gcttcttcgc gtttgccaag aattaatcca tcattccgcc cttcttcacg taatctgtca
    15421 gcaatggtca tcagtttctc cttttcctgt ggtgcgcgtt ctgctatctc accaataaat
    15481 gcacggaaac gctgggcatc tcccgtttgt aatacataat taaacagggc ttttagctgt
    15541 ctgtcattag tgttccctgt aactagcagc gaaacaattt ggtcgactaa tcccaacaga
    15601 tcgcgctgac gaatatgttt ctgaattaac tccaacagcg ccattttgcg atgttgcata
    15661 atctcgtcat ccggcaccac ggtaatatcc accaacggaa aagccgatga atatatttta
    15721 cgggcaatcg caggctcggc aaattcatca agccagcaga gtgaataagg ataaggactt
    15781 ctgcaaccat gataaaacag catcgggatc accaatggaa gctctttata gcccgcatca
    15841 agatggtttt gcattgccgc aatggaataa cgcatcatgc gaaaagccat taattcttcc
    15901 ggcttacttt ggtgctctat cactacataa atataaccca ctccctcctg cgttttcaca
    15961 gaccacaaga ggtcggaata atattgccgc aggtcttcat caataaaact gtttggttcc
    16021 agtttaagcg tcgttaaatc acacagtttg cgcagcggcg cgggaagatg aatatcaata
    16081 aaatcccgcg cggtgtctgg atggcgtaaa aaagatttaa ataccgcatc atgcggcgtg
    16141 gaagttgtcg atattgtcat ggcgttccgt cacccctcaa atttaagatg acgcgacaat
    16201 aaccacgatt aacacccaat gcagcaggca gtttttatct ttggacagcg aattcagaag
    16261 tatttttagc ggactgcaac ttttacattt cagcggaagc tgactcgcaa attccccttg
    16321 aaaatactcc ggcagcgtta gccaccggag aggcaattat ttcagcgaac ctttaaggaa
    16381 ctgttgcaga cgagggcttt gcgggttgcc aaataactgc tccggcgcgc cttcttcctc
    16441 tattttcccc tgatggagga aaatgacgtg agtagaaaca tgacgagcaa agcccatttc
    16501 gtgagtcact accaccatgg ttttcccctc ttctgccagt tgctgcataa tgcgcaacac
    16561 ttcgcctacc agttcaggat cgagcgccga ggtaggttca tcaaacagta aaacttccgg
    16621 ttccatcgcc agcgcccgcg cgatagaaac acgctgttgc tgaccgccgg aaagatgcac
    16681 cggatatttc ccctgtgcac gttcgtctat cccgactttt gccagatact tcaccgcccg
    16741 ctcgcgcgct tcctgcttgc tcaggcccaa cacctgaatc ggcgcttcca tgacgttttc
    16801 cagcaccgtc atatggctcc agagattgaa gtgctggaat accatcgtca ggcgtgtgcg
    16861 cagtaagcgc agttgatttt tatcggcgac tttgagttga ccgtctttgt cgcgcaccag
    16921 attgatcgtc tggccattga ccacgatcga cccttcactc ggtttttcga ggaagttaat
    16981 gcagcgcaga aaggtacttt tccccgatcc cgacgatccg atgatactta tgacatctcc
    17041 ggcattcgct tgcagtgaga cccctttcag cacttcgtgt tcgccgtagc gtttgtgcaa
    17101 atcgataacg tttaatttat tctcggacat cgtattctca gtgcgttgaa gaaggtttca
    17161 catgctgcaa ccagcgtttt tccgctctgc gaaagaggct gatcaggaca taagagatga
    17221 ttaaatagag caccgcggca atgccgaagg cggtaaaagg ttgatacgtg gcggcgttaa
    17281 tatcgcgggc tattttcagc agatccggca cagtggcagt aaacgccagc gcagtagaat
    17341 gcagcatcag gatcacttcg ttgctgtatg ccggtaacgc aatacgcagc gcagaaggca
    17401 aaataatgca gcgatacatt ttaaaggttg agaaaccgta ggctctggcg gcttcaattt
    17461 ccccatgcgg aaccgaacgg attgccccag caaaaatctc ggtagtgtaa gcgcaggtgt
    17521 taagcgtcag cgccagcacg gtacagttca ggccactgcg gaaaaaggcg ttaagaaact
    17581 cggtcccctt aacaatctcc agcgtataca tgccggaata gaacaccagc aactgaacat
    17641 acagcggcgt accgcgaaaa atataggtaa ataaccagat tggaaactgg atgtatttat
    17701 tactggagac acgaccaatc gccagaaaca gcgccaggac tccgcctatc actaccgaca
    17761 aaataagcag ccacagagtg atcgccacac cagtaaagcg ataaccgtcg gtccacagca
    17821 gcggtttcca gtattcatgt aagatttcga tcacaggtca gccctcttca cacccacgga
    17881 gtagcggcgc tcaaggaaca gcagcacacc attggaaacg gtggtgaaaa ccaggtaaat
    17941 cacgccacag acgatggcga aatagaacgg ttcccaggta cttttgcctg ccagctgcgt
    18001 ggctttgacc acatcttcca ggccgagtaa cgaaaccagt gcggtagatt tgaggatcac
    18061 ctgccagttg ttgccaatgc ctggcagggc gtaacgcatc atcgccggaa acatgatccg
    18121 cagaaacact tgcccacgag taaaaccgaa cgccgtcgcc gcctctatat gtcctttcgg
    18181 cactgccata aaagcaccac ggaacgtttc ggtaaaataa gcaccgtaga taaaaccgag
    18241 agtgataata ccagcgacca tcggatcgat atcaatctgc ccgacgccca tcgcctccgt
    18301 caccgtgttt agcgcaatct gcaaaccgta gaaaatcagc agcatcaaca ctaagtccgg
    18361 cacgccacga atcagcgtgg tgtacccttc gaaaatcagc cccgaaagcc gattttgcga
    18421 gagcttaccg ccagcgccaa ttaaaccgat gattacagcg agcactacag agctgatagc
    18481 cagctccagc gtgacgagcg caccctgtaa aataacacct gaaaacccat acaacatgca
    18541 gcctgtcctg tcgtgtgtgg tgaattaccg cctcttgtct ccctccggtt acccgaaggg
    18601 agcgggcttt ctttcaccga tggcgattag ccaccataaa catcaaaatc gaagcacttt
    18661 ttcgctaatt tctcgtaagt accgtcagcg cgcatttcag caaaggcttt gttcagtgcc
    18721 tcgcgcagtt cgttatcttc tttacgcagg cccatgccgg tccctacgcc aaacagtttt
    18781 tcatctttaa cagacgggcc accgaatttg taatctttac cgacaggttg tttgaggaaa
    18841 ccttcgctgg cagcgacctc atcctggaac gcggcatcaa tacgtccggc agtcaggtca
    18901 gaataaatgt tgtcctgccc ctgatacgag acgatttcaa tgccttttgg tgcccaatgt
    18961 tcattaccga acgtctcctg ggtggtgccc tgcaatacgc ctacccgttt gcctttcagc
    19021 gactcgactg tcggctgaat gtcagaattt ttcgccacca ccagacgaga atcggcagcg
    19081 tacagtttgt cggtgaaggc tatttcttgc tgacgttttt ccgtaatgga aagcgatgac
    19141 atgatggcgt caatcttctt cgcttttaag gacgggatta acgcatccag cggattttcg
    19201 acaaacgtac attgcgtatt gatgcgtttg cataattcct ttgccagatc gatatcgaag
    19261 ccaaccagtt cgccttgtga attttttgat tcaaatggcg cataggtcgg gtcggtaccg
    19321 atgcggatgt tttgcggaat cgcagcaaat gccgcagttg cgctggagaa ggccagaacc
    19381 agagagagcg atagcaccag ttttttcata atgtcctcaa ctgacagtct tt
//
LOCUS       DAZUCE010000081        19394 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZUCE010000081 DAZUCE010000000
VERSION     DAZUCE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.081"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..108)
                     /locus_tag="ACHY03_002419"
     CDS             complement(<1..108)
                     /locus_tag="ACHY03_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905637.1"
                     /translation="MSRHDILLRSQFERIIEGDRVGQALISFYEKLPEEN"
     gene            complement(254..4156)
                     /gene="hrpA"
                     /locus_tag="ACHY03_002420"
     CDS             complement(254..4156)
                     /gene="hrpA"
                     /locus_tag="ACHY03_002420"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFS3905638.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSNIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLGVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4357..4962
                     /gene="azoR"
                     /locus_tag="ACHY03_002421"
     CDS             4357..4962
                     /gene="azoR"
                     /locus_tag="ACHY03_002421"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFS3905639.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5016..6308)
                     /gene="ynbD"
                     /locus_tag="ACHY03_002422"
     CDS             complement(5016..6308)
                     /gene="ynbD"
                     /locus_tag="ACHY03_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFS3905640.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVSWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIKIALPYVVG
                     ACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(6322..8079)
                     /gene="ynbC"
                     /locus_tag="ACHY03_002423"
     CDS             complement(6322..8079)
                     /gene="ynbC"
                     /locus_tag="ACHY03_002423"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFS3905641.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLLLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILLYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8095..8991)
                     /gene="ynbB"
                     /locus_tag="ACHY03_002424"
     CDS             complement(8095..8991)
                     /gene="ynbB"
                     /locus_tag="ACHY03_002424"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFS3905642.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNTQGGALLVLFLLAITESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTSLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(8991..9596)
                     /gene="ynbA"
                     /locus_tag="ACHY03_002425"
     CDS             complement(8991..9596)
                     /gene="ynbA"
                     /locus_tag="ACHY03_002425"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFS3905643.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNLQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFFTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWGIASILLIWTAINRCRSVLLMSAER"
     gene            complement(9766..12072)
                     /gene="ydbD"
                     /locus_tag="ACHY03_002426"
     CDS             complement(9766..12072)
                     /gene="ydbD"
                     /locus_tag="ACHY03_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFS3905644.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALKIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIASMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDVYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLESALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12136..12996)
                     /gene="pdxI"
                     /locus_tag="ACHY03_002427"
     CDS             complement(12136..12996)
                     /gene="pdxI"
                     /locus_tag="ACHY03_002427"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFS3905645.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIEALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(15898..18642)
                     /locus_tag="ACHY03_002428"
                     /pseudo
     CDS             complement(15898..18642)
                     /locus_tag="ACHY03_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
BASE COUNT         4924 a         5202 c         4381 g         4887 t
ORIGIN      
        1 attttcctct ggaagttttt catagaaaga tattaaagcc tgaccaacgc gatcaccctc
       61 tattatccgt tcaaattgcg acctcagtaa aatatcatgt ctactcattg caatacgtcc
      121 tgttcgccca gggcgaagta tgaatgaaac ttatgtaaaa tagtaccgcc cttgctatat
      181 ttcagaattc ataaagaaaa aacccggcgc taaatgctta ccgggtttta tatttatcag
      241 gcaaatagcc gcgttaaccg ctaatctgct ccatcgcctg caaaatacgc ttatctgaaa
      301 tcggataagg tgtaccgagt tgttgggcaa aatagctgac gcgcagctct tctatcatcc
      361 aacggatctc tttcacgtct tcatcctcac gacgtgcagg cggcagtttg ttgatccatt
      421 gctgccacgc ctgctggacg ttttcgactt tcagcatctg agcacggtcg cgatgtgggt
      481 caacccccag tttttccagt cgtttttcaa tcgcctgcaa ataacgcagc gtgtcgccca
      541 gccgtttgaa gccgttacca gtcacaaaac cgcgatacac caacccgccc atctgcgctt
      601 taatgttaga aagccccagc gccatggtca tatccacccg ccctttcagg cgtttgttga
      661 tattgaacac tgccgtaagg atttgctcga cctgcttcgc aatatccacc accgtgtcgt
      721 tcagttcagc gcgaactttt tcatgcagcg cagcaaagcc ttcttccgtc cagaccgggc
      781 caccattggc gtcgatcagt tgatccacac cgcaggagat acagtcgtct atcagctcca
      841 gcactttgcc atacgggtta aagtacagcc ccagcttggc tttgtttggc aacttctcat
      901 gcaaatattt gattggcgac ggaatattca gcagcagtaa acggcgaaga ccgttccaca
      961 ttgcctgttt ttgctccagc gggttatcaa acagtttgat cgccacactg tcgcgctcat
     1021 ccaccagcgc cggccacgcc ttcactttgt agttaccacg cttctgttcg tagctttccg
     1081 gcaactgacc aaaactccag atatgtaagc cgctctgctc gataccgtca tccgccaccg
     1141 cagacagcgt ttcctgcact ttgcctttca gcgcatcttt cagatcttgt agcgagcgcc
     1201 cttcttttag cttcttgttt ttgtcatcca ccacgcggaa ggtgattttc aggtgatcgg
     1261 gcacctgatc ccagtgccag tcttcgcggt caacggtaac gccggtcatc cgccgtaact
     1321 cgcgctcaag gctgtcgagc aacggtaact ccaatggtgt tacgcggcct aaaaacgctt
     1381 cggcgtagtt tggcgcgggc acaaaattac ggcgtaccgg tttcggcaac gatttaatca
     1441 gagcaatcac cagttcgcgg cgcagaccag ggatctgcca ttcaaacccg ctttcctcaa
     1501 cctggttaag taacggcagc ggaatatgta cggtcacacc gtccgcgtcc gcgcctggct
     1561 caaactgata gctcaaacgc agcttgagat tgccctgatg ccagaagttc ggataatcca
     1621 gcttgctgat tttttccgcc ccttctttga tcaacatgct cttttcaaag ttgagcaaat
     1681 caggcgtttc gcggctgact tttttccacc agctatcgaa gtgacgagcg gagattacat
     1741 cgtggctgat gcgctggtcg tagaactcaa acagcgtttc gtcatcaacc agaatatcgc
     1801 ggcgacgtga tttgtgttcc agctcttcca cttccgcccg cagtttcagg ttttcacgga
     1861 agaatgcgtg acgcgtctgc cagtcacctt ccaccagcgc gtggcgaata aagagttcac
     1921 gacataacgc cggatcgatc tggctgtagt tgaccttgcg cgcagcaaca atcggcaaac
     1981 cataaacagt gactttttcc gttgccatca ccgcgccctg cgcccgttcc cagtgcggtt
     2041 cgctgtaggt gcgtttaatc aaatgctgag cgacgggctc cacccattcc gggtcgatac
     2101 gcgcagcaat gcggccccac aggcggctgg tttctaccag ttccgccacc attacccatt
     2161 tcggcggctt tttgaataaa ccagaaccgg ggaagatgga gaaacgcgcg ttacgtgcgc
     2221 cggtatattc ttgtttatcg gcatctttca tgccgatatg ggaaagcaac cccgtcagca
     2281 acgcaatgtg aatttcgcga tattccgccg gttcgctgtt caccggaatg ccgagttctt
     2341 tcaccacctg gcgcaactgg gtgtagatat cctgccattc gcgcacgcgc agatagttga
     2401 gataatcggt acgacacagg cgacggaagg cgttggaaga aagcgccttt tgctgctcgc
     2461 caagataatt ccacagattt acaaacgcga ggaaatcgga ctctttgtcg tggaagcgac
     2521 gatgtttttc gtccgatgcc tgctgtttgt ccatcggacg ctcgcgcgga tcctgaatgg
     2581 agagcgcgga cgtgataatc atcgcctcac gcacgcagcc gtgtttctgc gcttcaagca
     2641 ccatacgcgc aagtcgagga tcgaccggca gttgcgaaag ctgacgcccg agcggcgtca
     2701 gtttataggc gctggcctgt tcatccgtgg tgattgcacc caactcttcg agcagacgca
     2761 caccatcctg gatattgcgt ttatccggtg cttcgacaaa cgggaacgca gcgatatcgc
     2821 ccagccccag cgcggtcatc tgcaaaataa ccgaggccag gttggtacgc agaatttccg
     2881 ggtcggtaaa ctccgggcgc gagaggaaat cgtcttcgga atagagacga atacagatcc
     2941 cttcggacac acgaccacaa cggcctttac gctgattggc agacgcctgc gaaatcggct
     3001 caatcggtaa acgctgcact ttggtgcgat agctgtagcg gctgatacgc gctgtaccgg
     3061 ggtcgataac gtatttaatc cccggtacgg tcagcgaggt ttccgcgacg ttggtcgcca
     3121 gcacaatgcg ccgtccgcta tgcgactgga acaccctatt ctgttcgctg ttcgaaagcc
     3181 gcgcataaag cggaagaatt tccgtatggc gtaagttcag tttgttcagt gcatcagcgg
     3241 tatcgcggat ttcccgctcg ccgctcataa agatcagaat gtcgccaggg ctttcctgac
     3301 tcagttcgtc tacggcgtca aaaatcgcct gcaattgatc gcgctcggtg tcatcggctt
     3361 cttcaacaat cgggcgatag cgcacttcca ccggataggt ccgaccggag acttcgataa
     3421 tcggcgcatt attaaagtgg cgcgaaaagc gttccgggtc gatagtcgcg gaagtgataa
     3481 tgatttttag gtcaggacgc cgcggcagca actctttcaa atagccgagc agaaaatcga
     3541 tattcaggct acgttcgtgc gcttcatcga taatgatggt gtcgtactgc atcagcaggc
     3601 ggtcttgctg gatctccgcc agcaggatac cgtcggtcat cagcttgacc atcgtgttat
     3661 cacttacgtg atcgctgaaa cgcactttgt aaccgatgca accgcccggc tccgttttca
     3721 gctcttccgc aatacggttc gccaccgttc gtgccgccag gcgacgcggc tgggtatgac
     3781 cgatcagccc tttaataccg cgccccagct ccatacagat tttcggtagc tgagtcgttt
     3841 taccggaacc ggtttccccg gcgacgatca ccacctggtg atcacgaatc gcttcgagaa
     3901 tgtcctgttt tttctgacta accggcaaat tgtccgggta agtaatttcc ggtcgtgccg
     3961 cttcacgcag caggactttc cctgccgcct ggtcaatctc tttcgccatc tcctggaaaa
     4021 tggcctgttg tgcatcagga tttttaacct tcttcacacc atgcagacgg cgagaaaaac
     4081 gcagtctgtc acgcagcatc agcgaatcca gccgctgctg caaggctgta aaggtcaatt
     4141 tttgttgttc tgtcataacg ttagagggca gtaggctgcc tgattaattt tcttttaaat
     4201 gatagatata gagtaccaca tcgcggcttt ctatgcgttg ttcaataaat tcgaacatag
     4261 gcttcgattt attgcgctat ctctgtggca ggattgtgaa taaagtgtca gcaagcaacg
     4321 gggcatcgcc cattcaaaca tctataagga aacaccatga gcaaggtatt agttcttaaa
     4381 tccagcatcc tggcagggta ctctcagtct aatcagttgt ccgattattt tgttgaacaa
     4441 tggcgcgaaa agcactccgc tgatgaaatc accgttcgcg acctggctgc aaatccgatt
     4501 ccggtactgg atggcgaact ggttggtgcg ctgcgtccga gcgatgcgcc gctgactccg
     4561 cgtcagcagg aagctctggc actttccgat gagctgattg ccgagctgaa agcccatgac
     4621 gttatcgtta ttgcggcacc gatgtataac ttcaacatct cgactcagct gaaaaactat
     4681 tttgacctgg ttgcacgtgc aggcgttact ttccgctata ccgagaatgg tccggaaggt
     4741 ctggtaacgg gtaaaaaagc catcgttatt accagccgcg gcgggatcca taaagatgga
     4801 ccaacggacc tggtgacgcc gtatctgtcc acgttcctgg gctttatcgg cattactgat
     4861 gtgaaatttg tcttcgccga agggatcgca tacggtccgg aaatggcagc gaaagcgcag
     4921 tctgacgcga aagcagccat cgacagcatt gttgctgcat aaccggacac ggtcttgatg
     4981 tggactgtag atattcagtc cacatctcaa tccacttacc tgttttccca taatttcaac
     5041 atcgctttgt gctcttcctt aagcacaata tgcgagcgtc tggctcgaat aaagctaatc
     5101 gcttcatcaa ctgttttaca gtgtccgtaa cataacagcc atgccgccac taccagcgca
     5161 ctgcgcgata atcccaatgc gcaatggacc agaacgctgc cttgctcttc gcgtaatgtt
     5221 tccagcatcg ccacggcctg tcggagctcc ccctcttccg gaaccaccag atccaacatc
     5281 ggtacacaga aatagagtcg atcttttgtc gctcgtccgc gagggaattc aaaggtgacg
     5341 tccagaaccg cattctgtgc cggaatatgt cgtggaaacg cccctaaata aacaccagca
     5401 gtcattttgc tcaccggctc caggcgacga caaaaccaac gcatagatag ccacatcccg
     5461 atacgccagg gcaatgtcag ccagtaaacg gcgggcggga gtttcccctg actatctttc
     5521 cctgttgtta tcgcgccaag cccaccgtac ccacggccaa taatgagtag cgataatact
     5581 ggccaacata accagactga ccaccataac tgaaccatca tcattagctc catcaacaca
     5641 atgcacgcgc acgcacctac gacatatggc agtgctattt tgattcgacg ttgatcaggt
     5701 ttctgataat tccaacgacg gtcgacgggt accatccagt caatcaacat acctaccgcc
     5761 agccctgtga tgacatcaat aaaatgatgc tgccaggttg tcagcgtcga aatggcgatg
     5821 agtaaaaacc atccgccgca gactttacgc caactcacag ccagatgctg acgaaagtga
     5881 cgccaaagta gccagcagag aataatatgc agcgaaggag actggttata gggcagatca
     5941 aacagttcaa gttgcgaaaa tagccatcct gtcaccccac tcacttcagg acggataaaa
     6001 ctaaacttca gcgggtagag aaaaaaaccg cagcaggcca ttaccgttgc cagaataagc
     6061 cggtggacaa gtcggcgttg ttcgaatgtc gagctacaaa cgaacagcga aaatccatat
     6121 aaaagatcca gactccagta aggaacaata gtccagggaa gaaaagggat cgccgtttcc
     6181 caaccgaata cctgactggg gatatcatgg ctgttaaggt cctgaaccgc ggtgaactga
     6241 ttaagagatc cataggtgaa gaagaaaaac ggggccagca acaataacca gccagcgcct
     6301 tgtagcaata cgttccgacg ttcagttatc acgacgcacc gccatcgaaa ccgtaaaaat
     6361 accccactca tcaatccgtt gtgtgcattt atcaaatccg gcatcacgca cgagtgaatc
     6421 catctcccct tgcgaacgca cgcgcattac ccacggttta ccatctttat gactggttaa
     6481 caccccggca atcagctcca gttgtgggtg ccacggctgc ccggtgtaga ggagaatgcc
     6541 gcccggttcg atggcattgg caagacctgc gagtgagttt tttacctgct cattttcggg
     6601 aaaaagctca tacaggccag agacaatcgc cagcgtaggc cgcggagtta acgcgctgag
     6661 ttcctccggg ttaaacgcat cgccctgttc aaaacgcacc cgcccagaca ttccccgttg
     6721 agcaatcatc tcttgcccct gtgcaacatt taactcgctg taatcacgta acaaaatatc
     6781 gcttacggca ggctcgtttg ccagcgcatc cagtacatag cgcccatgcc ctgcggcaat
     6841 gtcaaccacg cggacggcta aacctttggc gtggagatcg gcaacggctt gtttaatcag
     6901 tatttgtaaa tgggttttgc gctggcgaat accgcgccag ccaacactgt tcaggtagat
     6961 tttgtcgatt aagcgcccga atgcgttact accttgcggt tgattttgat agacataatc
     7021 cagcgaactg cctgaatcaa agccggtgct cattccgaga tgcaggcccg cagagtgcgc
     7081 cccgaacaat ttcatcgctt tacgcataaa gcgatacgcc aaatcaaccg gcgataatgg
     7141 cacgggtcca cctgaaagga gccgccagcg atccgctgat ggtccagtgc ggtcttcatg
     7201 ctgataatca aatttttgtg acttgttagc atataaacga ctaataaagc tttgcatttt
     7261 ttcaaatgcc agcgccctgt tctcttcacc caacgtgtcg tgataaaagc ctggcagcag
     7321 atgcagctct ttgagagggc tacgtaaacg ctgataaaaa tcgatctgcg gttggcgatg
     7381 caccacatag tcatcgcctg atatcagaag ttgcgtgggg agcgtaatcg ccgccgcatc
     7441 gctaacaata cgttcagacg ttttatagag atcgagcaag atattgacgg caatcgcccg
     7501 tgtgatcagc ggatcattat tgaaactcgc cacccgttgc cgatcgtggg tcaaatagcg
     7561 tcctttcaca taggaattaa taaaaaacag accacgcaga cgatgccata acgccagcgc
     7621 aggacgtgcc agcggcacat acaatttaac cttaaaggcc ggagaagcca gcagcagccc
     7681 gcgaattgca ggcgcataat catgaaccca tgtggcaacc agcactgcgc cgacgctctg
     7741 cgcgatcact accacctctt ccagcccgac ctggctgtcg ctggcagcaa aacggacaaa
     7801 ctcatcgaca tcccgcactg aacgcgcaag agatgggcta taaccacggg gccccgaagt
     7861 ttgtccatgc cctcgggcat cccatgcata aaaagcagta tctggcatcg ccagttcatc
     7921 aacgatatgt tgtagacgac cagaatgttc atgaccgcga tgaaataaga cgatgacctt
     7981 tttcgcccct ggctgtaaag cgggccagtg ccgataaaac aacgctgtat tatcactggt
     8041 ggtaaaaaaa tgttccccag ggatgcgtga attttccatt ttctctattt tccttcagta
     8101 acagcagtag cgaataaaat aaaaaaatac cggagcggtg aaaattaatg agtcaatcct
     8161 gtcaaggagt ccgccatgtc ctggtaatag ttttccgcta tctttaacac ccacatctcg
     8221 tttgacggct gacatcacta catcgccgca aaaaccacta ataccaatta acaaacccgc
     8281 taataatgcc tgtaatgtat ttagcgatgt cagcaacggt ccaataatca gtgacgcaat
     8341 catggtggtg attacgcccc ccaccagacc ttccagcgtt tttcccgggc tgactttggg
     8401 gaccactttt cttctgccgc aggatttccc ccataaatac tgtgcaatat cgtttgactc
     8461 tgttatcgcc aaaagaaata acaccagtaa cgccccgccc tgggtatttg tggttggcaa
     8521 cattaataac caggcggcat gactcaaggc gaaaacggtc attatccagc cccaaaaaat
     8581 ggcgctcacg gtatgcagaa aaccggaggt atcacctact aaaactcgcc aggtggttaa
     8641 tatcagaaac ccagccaaag gtataaataa cagaaacagc tcaaagcaat taaaaccgat
     8701 aagcaaatag ttaagaggaa taacccaata taaccaacgc ggaaaatgta cggatataag
     8761 cgtacaatat tctttcaatg ccagaaaact tatcagcgca aaaaacgtca acgtcatcca
     8821 gcgtggacct gaaatcacca gtgaaaaaca aatgacgata ccccaccagg tacgaatacg
     8881 taatgtaact tcatcctctt ttctctccgg taaccgccat acccgaaaac ggtttatcag
     8941 cgttgctaaa attaacaacg cgaaaagtgt tgccagagat ttttccaaca ttatctttca
     9001 gcactcatca ggagcacact ccgacaacga ttaatcgcag tccagataag cagaattgaa
     9061 gcaatgcccc ataaaagatt attccattgc atccactgtg gataaatggc aacggccagc
     9121 ccccacaacc caaatattaa cgcacgatcg cttttgccga atggaccggc ataactgcga
     9181 ataccgttaa tcgtctgcgc gagtaaaccg caaaactcag tcaatatggt gaaaaataac
     9241 atgagtatta cgagtgatgc gttactttct ggtaaaaata aaaacggtaa atagagcgca
     9301 atatcggaaa taacatcgcc agtttcattc aaaatcgccc ccagacgtgt ttgcagattg
     9361 cactcacgcg ccaacatgcc atccagcgca ttgagtgcca tacggataaa aagcacgatg
     9421 ggcaatagca aaaagaggat gggttgtgcg accaacatca acagcaatcc ggtaataaga
     9481 gaaagcgcca gtgcagcaag agtgatgtga ttcgctgtaa catggtgctt ataaagccaa
     9541 aacatcgtcg gccttaacag cgactgaaag agcggtttta tctggtagag cgtcattatc
     9601 aatccttgat aattcggttt gcgctatggt gcatcaattc ccctgacgga gatatcgcaa
     9661 cacgacgctt cattaaaaat tcagaatata tcaaggtatt ggggactcac tgtcaggaat
     9721 gattatccca ataaatcaag tggttaaaaa ttattacgct gttattcaat acaactcatc
     9781 aagttttctc atcgcttgtt cccgtattgc gcgcgtctta ccgttaagcg ccagctggcg
     9841 aactaaatcc agttcagggt taacaagctc taacgccagc aaaggatcac gtttcagcct
     9901 cgcctttaat acatcaactg gaaaacgggg attattcatc gccacaatcc accactcggg
     9961 atcaatttct gccgccagta ctgcaggcgg tgtgtggcgg ttgttaagtg cgccgaactt
    10021 gaaaaatgtg agattgcttt tcgccagtgt actccaccat atacgatccg gttgttttgc
    10081 cagtacatcc agttgtgcta acgcatcggg gtctagtgca taataaacac gtaacgcttc
    10141 gttaacctgc gcacgggaac tgttttcctg cctcagaaaa aacttcgccc aaatatcatt
    10201 acgtggcgtg ttttgctgta attccttgag aacaacagcg ggtaaattat cccaactaat
    10261 taatgcactc tctaaaaagt cccagtcgtc cgaacgtatc agagaggtgg catttaatag
    10321 catctggtca tcaacttcac cgtagctgat aagctcagac aaaatcatat cagcgctggt
    10381 ttcatatgca tctgaatctg ctttgggatt atttttccga tgcgataaag cacgttgcca
    10441 aattttttgt ttcatcgacg tgctcagata ctgatttttc gccagattac tccacccctc
    10501 aggatcgttg aaacgtgtca ataaccgttg taaagcagcg tcagagtgaa ttatctgcat
    10561 atcccgacag caaaaatagg caggggattt tttctccatc cttttaacaa aattaaactg
    10621 taagttatct ggcaacgcta aaaataacgc acctattaga tcatcgtcat tctcgaattt
    10681 ttggtataaa tatactgcta ttttgtcact ctcttgtggc gtcatccgca acaattgacc
    10741 atggtgtttc atctctgcca gctttaacgc taagcttgtg acaacatcgt ttgatgaatc
    10801 gttcgccaac tgcattgcct gattgtaggt gagttgtcct ccagcaccga cctgacgaat
    10861 agaggaatca tcactgcgga tatagacatc aataatggat tcgggcaata accacgcatc
    10921 tttataaagc tcctgaatga tcatatccca atctcttttc gccagagtca tgacgatatc
    10981 cagagggaga ttttcacgta gcgcaagatt caacggaccg acaccatcag gtagatgact
    11041 cattcgcgtc attatttcga cagagagcgg ttcacggtcg agcaacccca gaacaacagg
    11101 ctggtaaccc aattgctgat cggtcaactc cagaatctca ttgtcaggca tatatctcgc
    11161 catacttgca atcgtttgtc catcattgct attaataagt tgtttgcgta ggttagctga
    11221 aattccttta agccttggga gagttcgcat tatggcaaga tcaaatgtcg caatatctcg
    11281 tccttcaaat gcagcaacgt tgccttcatc cagcatttgc aattcctgca ctaactcagg
    11341 agaaggctta gggcgaaaca gcgcttcttt tttcatctga ttacagacat tccctgccat
    11401 gcgaatattg cttcgcttaa aaatttcttt cagagcatct tcatcattta ttagacccca
    11461 ataatatgca tcaggattta ttatcttcag cgcttttttg acattttcag tgtcattttt
    11521 gtcactcgca cggaccatga attcactatt tgtcgaagac gcaacaacag gtgcaattat
    11581 gtcattgata aatatcccac gcgcattaat actcccctta atcgtagacg gattatcgta
    11641 atatgagtcg ataaatacca gtccacttgc atttatcgaa ccggtaatta acagtggtga
    11701 atccgtaact atattcgttg ccgtcacgtt accgagaata acccatggat tagaaagaga
    11761 gaaagtggaa atattacctt tgactgtcaa attgccaaga acaatcaatt tttgattact
    11821 ctctatatta ccttctacca ctaaatcgcc acgatgcact ttccagtccg atgcagataa
    11881 accgaagggg acaagattca cgctactgtt gatagcttgt tcaacttgtt gtttatcttc
    11941 agcggtagcc gcagaaaaaa aaggttcaat atcaactgca tttgcaacgc atcgaagttc
    12001 cgcctgtact ggaagactca gcataaacag gggaagtaaa cctatccagc tgcaaatcct
    12061 gggcttaaac atatgcactc catgccatta aaaaagaaac aaaagggaaa tgcatctttc
    12121 ccttttgtat attcgttatt ctcgcgaaat accatccaac gtagacaaca cttcctcaga
    12181 aagatgcaat ttttcagcag ccatattctc ccgtaaatgc gcaacagaag acgtccctgg
    12241 gatcagcaaa atattcggtg aacgttgtaa cagccacgcc agcgccacct gcattggtgt
    12301 tgcacccaag ctcgcagcaa catcggaaag cgtggaagat tgcagcggcg taaagccccc
    12361 gagcggaaag aacggcacgt aggcaatgcc gtcacgagcc aaagcttcaa tcattgcgtc
    12421 atcagcacga tgagcaatgt tgtattcgtt ctgcacacag acgatttcgg caatcttgcg
    12481 cgcctctgca acctgcgtcg gtgtgacgtt gcttaggcca atatgtttta ccaggccttg
    12541 ttgttgcatc tctgccagca cggtcaggct ggcttcaatc gatccttccg ccgggccatg
    12601 accatccccc atcatcacgc gcaggttaac cacatccagt acgtccaggc cgagattacg
    12661 tagattatcg tgcaccgctt tttgcagctc tgccggagaa aatgcgggca accaggatgc
    12721 gtcctctcca cgccgcgcac caattttagt gacaattgtc agatcgtcag agtaaggata
    12781 aagcgcttcg cggataatct gattggtgac gtgcggacca taaaagtcgc tggtatcaat
    12841 gtgattgacg cccaatgcca gcgcctcgcg cagcacggta atagcgacat ggcgatctct
    12901 gggggggccg aaaaccccag gacctgccag ttgcatcgcg ccataaccaa gacggttaac
    12961 ggattttgta ccgagagtaa atgtattgct gctcatcgca ccctgccctg taataaaacg
    13021 gattaagaaa aatcagtata gaaacgaaaa gagataaata atgcgaaggc gtaaacaaga
    13081 tttctggatg agtagcacta ttgtactgaa agacaccaaa acattagaca gcaaaagata
    13141 agtcaagcgt cgcatccggc aataaaaaac cgcaacctgt atcagcgttg cggtttgtgt
    13201 atcacatgct aaaaattaga acgttttctt aaagttcagc atcacgcctt tgtcgctgat
    13261 gccgtcctct ttccagtgat acgcatccag attcagcgaa ctttctttgc tactgtactt
    13321 cacgccgacg ctcgccaggc tgttgatacc gccgcccttc tgaccgtcat cgacgttaaa
    13381 gtgctggctg cctgcccctg caaggcttgc cgtgcgctgg ctcttcgcgt agctcaggtt
    13441 cggcccgcct tccagcgtag cgctagcgct ccagccgtct ttacctgcgt aatccagttt
    13501 caacccgacg atgctgtcca ccgccgtttc gctgccactg ttcatattca ggttaaagtc
    13561 tccggcattg cgctcctgat agccaccttc cagtgtgtga cgcagtttta cacccgcata
    13621 tggcgtaacc ttcagtcctt cgctcggttc gaaagtcttc gccccttcgc tgcggaactc
    13681 caggtactgc tgcttcacgt cggtatcagc cgttttgttt gtgttgctaa atgcaatcga
    13741 acggctactg tcaaggttgt gaacgtcata acgcaatgcg ttattccagt tcatgccgtt
    13801 gtcgaaggac atctgatgtt tcaggccaaa gaactggcta taaccacctg tgacgccatt
    13861 atcacccgct ttctgcgcac catcaccatc gagacgagcg ataccgtact ccagactcat
    13921 cgtctggctt tcgctcaggt cgatggtttt acgcaatgcc agcatgtcgt attcggtatt
    13981 attacctaac tcggcacgtg gatcgccttt cgcaacaacg ttgaacgcca gaccgttacc
    14041 caccttcggc gcggcatccg ccagcatgct gaagcggttg cttaacacac gcgcttcacg
    14101 gaataccgtg gtcgcctggc taccgctcac ctgcttcagg gcgctattca gttcagcagt
    14161 ggtgcccacg ttcaggctgg tatacagctc gttattggtg taacccgcat ccagtgcctt
    14221 cgccacatcg ttcaccgaag catcggtcgc cacatcggtg taggcgtttt tgctcatgat
    14281 gacgtcaacg ttgccgctgg catcggtgct gcctttggcg gtccatacca cggacgttga
    14341 ggtgatggca tcggcgtcgg tcaggttgat gccttccacc acgttgtcga aactgaccgt
    14401 ggtgtctgcc gtaccggcgg cgaacccggt gttgatcccg acaccattca tgctggcgtt
    14461 gttgaccttc agtttaccgg cgctgccatc aacgttggta ccgacgatat agccgttaag
    14521 attgttactg ctgccatcgc tactaccgct atcagtcgtt tcactgaaag gtgaaatggt
    14581 gtaagagctt ggcatgtccg gcaacgtgta gtcggtgtaa tgcacttcgg tattgttacc
    14641 gctgttgccg ttcacccctt ccacattcac gctgtcgccc tgcttaatca accccgaacc
    14701 ggtcacgcca tcggcaatca ccaccgtacc attgttaacg atatggcctt gtgttcccaa
    14761 tacactgaat gcaaacgaat cgttagcata gatattaatc gtgccattgt tctcaatcac
    14821 cgcgtctgcg gtagcgttgg catccagttg cataccagtc aacccggtat cggtggtgcc
    14881 ttcggtgccg agattgataa caccgttgtt aaccagttgt gcgtttttat cgccttccat
    14941 cgccacggca ttgttgccgc tgatgttcat tacgccatcg ttcagcgcaa catagttgct
    15001 gtgggtgaac ttaaccgcgc tggcgttatc tttatcgaag ttgatgaccc cgccatcctg
    15061 gttccacaag aaggcatcgc tttctgtcgc accggcagta cttaccgcca cggtgacttt
    15121 cgcatccacc acgcccttat taataaactg gctggcgcca tcgtcgtagt gaatagcgcc
    15181 cgcacctaat gcggcatcca gcgtcatttc accggtgtta tataacgtgg cgttagcatt
    15241 gctgccctgg aagcgaatgg cgtttttgct gttatcaagg atgagtttgc cgctgttgtt
    15301 gaacgttgac ttatcgtcaa tatccagcgt cccggcattg ttgttttcat cctgataacc
    15361 cattttccag gtacccgcgt tattcactac cgcagagtta tagagaatcg cgcctttacg
    15421 cgcctcgccg cctgcaaggt tattgaatac cgcatcgccc tgagcgttaa cgcgcgccga
    15481 cgcaataccg ctgttattca ccgtaccatc gttaagctgt aaggttgaca gggaaccggt
    15541 gttattaatt accgcgttgc tgtttttctc aattttgacg acgtcattaa gcgtgccgtt
    15601 gttattaaag gttccccctt ccagagtcac cgtgttcaac acaccctcat tggttaacgc
    15661 accgcctgca ttcacgacga tggccgaatt gattgctccg ctttcctcgt taaccagcgt
    15721 gccgttttcg agtatcctga tcgttccatt tttaacggta cccgcactgg tgacatcccc
    15781 gttgataatc gcacctgacg ataataatac ggtctcaccc gcagagctta atacatcgcc
    15841 atcatcatac aaatcggcga tataattatc cgtcggatta tactcatcag ccccctggca
    15901 aacaccaaag gtacaaatgg tgccatagtt gataataagt ccagtgccat cgttatagaa
    15961 tgcctgacca ttttccgcgt tgatgttaat tacgcctgtc tggtcattaa tggcggtgcc
    16021 ttcgtgataa accgccatgc caacgagttt attcacaccg agggaatcgt tgtaattttc
    16081 atttttctcc aggttgatcg taccctggtt aatgaccgta ttggtattac catacgccgc
    16141 catccccgca cccgcgttgt agacggtaat tacgcctgtt tcgtggttgg tagccgtggc
    16201 tttggttaat gccccattat aagcatcggt acccgaggcc attcccacac cgaaatcttt
    16261 cgctgtgcta ttggcgatat tattacgcag cgatgtctca tcattttcat cgacccacag
    16321 cgtatccagg gtgatttgcc cttcgttatc cgcggttgat ttatcgatga ctaacatccc
    16381 cacgctacca cggccataca tatcaataat gcccgtttcg ccgttcagaa tggatgacga
    16441 gtagctgcca agcatacctg tcagccctaa tgctttgtta ttgaaagagg taccatcaga
    16501 caagttccac tctgccggat aattcagcac actgtgctga ttcatcattt tggtggtcaa
    16561 ggtgccattg ttaacgccaa tacccccata ttcaacgtca acgacttttg gcgccgggtc
    16621 ggtaccaaaa tcgtacatcg ccaggccaat ggatccgtta ttgatgaccg ttgaatcttt
    16681 attccaggca gccgcaaaac cgatattttt cagttccact tcaccgttgt ttgtcgcctt
    16741 cgcaccagaa tcaatactaa tggtggataa cgctacgcga taataatagg tgaaggaatc
    16801 cacatcgctc atatcaaacg ttgcgtcagc gccaatctcc agctccgcgc cattactcag
    16861 ttgaatgaga ttttcgaaac ccagcgggaa ctcaccgctg atggttgact caccattgag
    16921 gtagactgag gaatgcccat caacgctgat aagcggtgta tctccgatac cacgacgaga
    16981 tgcggcaggt tttgagccat catccatgac atttttttca ccgacaaaat ggacaccgcc
    17041 attaagcgtg aaggtattgt catcgccagc aataacaata cccgcaatgt gctcctgagc
    17101 accgtcgtaa cttgcatagg aacgcgtagt ggtttcgcta tcagcaacaa cggttaactg
    17161 accatccagc gtgacgtcat tgttattacc gctgatattt acgccggttg agggattaaa
    17221 gaagtaaggt tgcgcattat cggctttctg atctttatca accagaatgc tgcctgcgat
    17281 ttcaatcgta ttgccatcac ccgaaacatt aacacccgtc gctttttgcc ctgtcacatt
    17341 cagctcataa gtcgccagcg tcatgttgtt attattgcca gagacatcaa gaccggtgga
    17401 gaaatcccca acgtctaacc cacccgcgag tgaaatatta gctttatccc cagcaacgct
    17461 gacaccggtt ccggcattgg tcgcggaaat atcaccaacg ttaacaaatg tcgcattatc
    17521 accgtcgatc tgaatgccaa tggcattttc acccgctgcg gcctgaccaa cattgaggtc
    17581 gcccatgttg atcacgctga cgccatcacc atcaagcagc atgccaatag agttatcttc
    17641 gatgaccgta atattacctt tgttgctaac gaacgtatca ttgccgtcaa cgtcaatacc
    17701 gtgcgcacca ccgcttacgt ataagtcgcc ttcctgtttg atggtggcac cgtcaccgac
    17761 aattttcgag ccagtggaat tctcaccgtc aacactcatg gtaccttgat tatcgacccg
    17821 cgcattatcg ccattgataa ggctacccgt accaccattg gtgatggtcg agtcgcccgc
    17881 agtgttcagc gtagcattat cgccattgat gattactgcg gtaccgccgt caacttccgt
    17941 ctcgccgttg ttattgatag tgatatcgtc accagtaagg ataccaacga ccgaaccctc
    18001 gccgatggcg gtcgttttac cgtcgttgtt gagggtgatg ttgtcaccct caaccgtcat
    18061 gacagtaccg ccattcagcg ccgtattgcc gccctgatcg gtgatgatcg cattaccaaa
    18121 atcaatcgca gttccggttg tgccatcaac aacgacaccg gcctctttag tgatatggat
    18181 gagcccatca tcgtcgtagc tccaggtgat gccacctgcc gtatttacgc catcaaagat
    18241 gacggtgttg ctgtcgtcat ggactgtcca gcccttgact atcgtcgtca ggccatcagg
    18301 cgcggttaag gtgtaactcc catcggcatt ttggctgtag gtaaaggtgg tttcgcgaat
    18361 tttgacggtt ttcgcgtcct gatcccaggt tacgtcattt ttatagacga caggatcgtg
    18421 tttaaccggg gtatcgccac catcgtctgg cggaacaggc gtatcaccgc cgtcgtctgg
    18481 aactggcgta tcaccgccat catctggtgg aactggcgta tcaccgccat catctggtgg
    18541 aacaggcgta tcaccgccgt catctggtgg aaccggcgta tcaccgccgt catctggcgg
    18601 aactggcgta tcaccgccgt catctggcgg aactggcgta tcaccgccgt catctggcgg
    18661 aaccggcgta tcatcgccgt catctggagg aactggcgta tcaccgccgt catctggcgg
    18721 aactggcgta tcaccgccgt catctggcgg aaccggccta tcaccgccgt cgtctggcgg
    18781 aacaggcgta tcaccgccgt catctggcgg aaccggcgta tcaccgccgt cgtctggcgg
    18841 aacaggcgta tcaccgccat catccggaac tggggagtta ttatggtggt gattgtcatc
    18901 gtcacttaag gtatatatag caattcccgt agctactgca gcaagacctg cagctgccca
    18961 tggtaaaact gcactatctt tattttctct gcactccgcg gataaatttt ctagctgctc
    19021 tttgctcagg gaatgaatat ttgccggaca tgttatctta ctggacttac gtgttttttc
    19081 ggttgagtcg ggtattgaag tatccgcagc ccatgtctgg ctacttaacg ccatggcaac
    19141 acaaacagac aataattttt tcctttgcat ataaaattcc ttagatctag accgtgcgta
    19201 acaagccatg cagaatttga agataaatcg tagtaaatat tcatagtgaa ataagcaata
    19261 aagaaatgct tatataattc acaaaatcaa tattaaaatc aggcaagtaa aagcatacaa
    19321 aaaaacagtt gatggaatta tattccacaa aaaactcact tcaatctaaa aattaacgac
    19381 agaaaacaaa aaca
//
LOCUS       DAZUCE010000082        19043 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZUCE010000082 DAZUCE010000000
VERSION     DAZUCE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.082"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..258
                     /locus_tag="ACHY03_002429"
     CDS             <1..258
                     /locus_tag="ACHY03_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS3905646.1"
                     /translation="EQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGNGFKLDSVD
                     ATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            294..1454
                     /locus_tag="ACHY03_002430"
     CDS             294..1454
                     /locus_tag="ACHY03_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HFS3905647.1"
                     /translation="MKSLAEFHLKNNAVMGVYNNRTLPSSYHDAMTEYKAVRENALLV
                     DYSHLSIVSVMGDDAWALINQLVSADVSIIRDEQAIYSLVLNEEGTIRGDVYVLCSID
                     GYYLLSEDISATELIASMNTILEKAEELDIQSLPEIQDMRENNWGAILLEGPYSWEIM
                     SEIHGFDVIGLPYYEYMNTDEDLLIFRCGKHGEYAYMTIGEQAKLAEQWEKLLTVGEK
                     YLMQTGGLDYQKIVRLENPCWDASLWEGQSVNPVQLQMQWAVQYDKDDFIGKDAVTEL
                     SQEYTGNKLIGMIAQEECEGIEAGDRVLVEGQDVGYVVKAVFSPALQRFIALTLLEKD
                     YAWSDISGYEIQTAHGIIPAQSKCMPFIYNLSMLVSPTEHSYIDASKNKSAA"
     gene            1528..4086
                     /locus_tag="ACHY03_002431"
     CDS             1528..4086
                     /locus_tag="ACHY03_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HFS3905648.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVADSERFA
                     KWFENARAAEVLHDIEYSDLNDDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHAVARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSQARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSDATLPETASNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAVARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            4091..5314
                     /locus_tag="ACHY03_002432"
     CDS             4091..5314
                     /locus_tag="ACHY03_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HFS3905649.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHAAKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESAHHRRARQQSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAIAMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMSNMQAGRAK"
     gene            5311..6264
                     /locus_tag="ACHY03_002433"
     CDS             5311..6264
                     /locus_tag="ACHY03_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS3905650.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGSFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKRLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERQQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            6275..6982
                     /locus_tag="ACHY03_002434"
     CDS             6275..6982
                     /locus_tag="ACHY03_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3905651.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNIFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            6966..7622
                     /locus_tag="ACHY03_002435"
     CDS             6966..7622
                     /locus_tag="ACHY03_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905652.1"
                     /translation="MTQMHKGFLLIGVFLYAAFLAPKIYAATPDAVNQVLQQGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWTTTIRDHILAM
                     RYRALRHCKDTTRANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            7623..8933
                     /locus_tag="ACHY03_002436"
     CDS             7623..8933
                     /locus_tag="ACHY03_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3905653.1"
                     /translation="MSGEILAVNSVLLLILFFVFYQLLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLATDISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKMRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDGTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            8942..9730
                     /locus_tag="ACHY03_002437"
     CDS             8942..9730
                     /locus_tag="ACHY03_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HFS3905654.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDDKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGATQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKVM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLMGEVPCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            9727..11115
                     /locus_tag="ACHY03_002438"
     CDS             9727..11115
                     /locus_tag="ACHY03_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905655.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMSVDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSYSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHHETAQVEEYQFPDSLYETKDESGVELALGGQYTSDSFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSAISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            11129..12070
                     /locus_tag="ACHY03_002439"
     CDS             11129..12070
                     /locus_tag="ACHY03_002439"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFS3905656.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMLNDFAGVDQQRVMHCRKAFTDAKVFK"
     gene            12067..13053
                     /gene="ycdU"
                     /locus_tag="ACHY03_002440"
     CDS             12067..13053
                     /gene="ycdU"
                     /locus_tag="ACHY03_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFS3905657.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFANPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(13246..13333)
                     /locus_tag="ACHY03_002441"
     tRNA            complement(13246..13333)
                     /locus_tag="ACHY03_002441"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13297..13299),aa:Ser,seq:gga)
     gene            13567..14505
                     /gene="ghrA"
                     /locus_tag="ACHY03_002442"
     CDS             13567..14505
                     /gene="ghrA"
                     /locus_tag="ACHY03_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFS3905658.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            14560..15297
                     /gene="ycdX"
                     /locus_tag="ACHY03_002443"
     CDS             14560..15297
                     /gene="ycdX"
                     /locus_tag="ACHY03_002443"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFS3905659.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            15321..15875
                     /gene="ycdY"
                     /locus_tag="ACHY03_002444"
     CDS             15321..15875
                     /gene="ycdY"
                     /locus_tag="ACHY03_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFS3905660.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            15977..16468
                     /gene="ycdZ"
                     /locus_tag="ACHY03_002445"
     CDS             15977..16468
                     /gene="ycdZ"
                     /locus_tag="ACHY03_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFS3905661.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(16532..17365)
                     /gene="csgG"
                     /locus_tag="ACHY03_002446"
     CDS             complement(16532..17365)
                     /gene="csgG"
                     /locus_tag="ACHY03_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFS3905662.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(17392..17808)
                     /gene="csgF"
                     /locus_tag="ACHY03_002447"
     CDS             complement(17392..17808)
                     /gene="csgF"
                     /locus_tag="ACHY03_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFS3905663.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(17833..18222)
                     /gene="csgE"
                     /locus_tag="ACHY03_002448"
     CDS             complement(17833..18222)
                     /gene="csgE"
                     /locus_tag="ACHY03_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFS3905664.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(18227..18877)
                     /gene="csgD"
                     /locus_tag="ACHY03_002449"
     CDS             complement(18227..18877)
                     /gene="csgD"
                     /locus_tag="ACHY03_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFS3905665.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         4734 a         4374 c         4947 g         4988 t
ORIGIN      
        1 gaacagcgta aagaagttct gatgaccatt aaaactgaga tcatccagcg actgaatatt
       61 caacgcgatg aagcgcaaat tgatgatgat actgcgctgt ttggtaatgg ttttaaactg
      121 gactccgtcg atgcaacgga aattgtcgtt ctgctggata aagtatttga tatcaagatc
      181 agcgaaagtg acgacccgtc ctatatgcgc agtatcaata gtctggccac cttcgttatt
      241 caaaagaaaa acgattaact acttattaaa gaattaatta tcaggacact tttatgaaat
      301 ctcttgctga atttcacttg aaaaataatg cggtaatggg ggtatataac aaccgtaccc
      361 taccttcttc ttatcacgat gctatgaccg aatataaagc cgttcgtgaa aacgcgctgc
      421 tggtggacta ttctcacctc tctattgtta gcgtcatggg cgatgatgcc tgggcgctga
      481 tcaaccagct ggtttccgca gatgtctcca tcattcgtga tgagcaggca atctactcac
      541 tggtgttgaa tgaagagggc accattcgcg gagacgtcta tgtgctgtgc agcatcgacg
      601 gttattacct gctgtctgaa gatatttcgg ctaccgaact gatcgccagc atgaatacca
      661 ttctcgaaaa agcggaagaa ctggatattc aatcgctgcc tgaaattcag gatatgcggg
      721 aaaacaactg gggggcaatc ctgctcgaag gcccatactc ctgggagatc atgtctgaaa
      781 ttcatggctt tgacgtgatt ggcctgcctt actacgaata catgaatacc gatgaggatc
      841 tgctgatctt ccgctgtggt aaacacggtg agtatgcgta catgaccatc ggtgagcagg
      901 cgaaactggc ggagcagtgg gaaaaactgt taaccgtcgg cgagaaatac ctgatgcaga
      961 ccggcggtct ggattatcaa aaaatcgtac gcctcgaaaa tccgtgctgg gatgccagcc
     1021 tgtgggaagg tcagtcggtg aatccggttc agttgcagat gcagtgggcg gtgcagtacg
     1081 acaaagatga ttttattggc aaggacgcgg tgacagagct ctcccaggag tataccggta
     1141 ataaactcat cggcatgatc gctcaggaag aatgcgaagg cattgaggct ggcgatcgtg
     1201 tgctggtgga aggtcaagac gtgggctatg tcgtgaaagc ggtattttcc ccggcattgc
     1261 agcgtttcat tgccctgacc ctgctggaaa aggattacgc ctggtctgat atcagcggct
     1321 acgaaattca aactgcccac ggaattattc cggcgcaatc aaaatgtatg ccgtttattt
     1381 ataacctgag catgttggtt agcccaactg agcacagtta tatcgacgcg tcaaaaaata
     1441 aaagcgcagc gtaatgaata tcgccgcgtg actatagctt acgcggctta aattaacccc
     1501 aattggcggg attgccaggt attattcatg aaaacgaaca ggaaaagagt tgttattacc
     1561 gggctgggga ttttgtcctc actggcggaa aatattcagg atttccggga agcattatta
     1621 aataaaaaaa atggcgttgc ggatagcgaa cgctttgcaa aatggtttga aaatgcgcgt
     1681 gcggcagaag tgttgcatga cattgaatac tccgatctga acgatgatgt tatcgattca
     1741 ctggataacg cagcgctctg ggcctacaaa gtgggtaaag atgcgctgag tcaggccggc
     1801 ctgatggata gccgtgacac cctcaaagaa acaggcatga tcgtcggtgt ttcctccgcg
     1861 ggtacagagg cctttttacc gctgttcgaa cagcgcatgg aagatttctc gctgcgtaaa
     1921 gcactctttt ccggcgcatt ctcatcctgt tgttccagcg tttcaacgct gctgggctta
     1981 cagggtgggg ttgaactggt cgcgaccgcg tgtaccgcca gcccgaacgc cgtggggatg
     2041 gcctttgact acatccagaa tggtaaaagc aaaaccatgc tggcagtagg taccgagccc
     2101 atctatctgc cgacctttgc cggattctac gcactgaatg tcatgcatcc agacacttgt
     2161 acgccgttct cgggtcagtc cggtatgtcg attggtgaag gtgcgggcgc tatcgtactg
     2221 gaagagtatg agcatgcagt agcccgtggg gcgaccattt atggtgagat cctctcttac
     2281 gccacttcct gcgatgcttt ccatgaaacc gggccagatc cgcgtgccag cggagcggtg
     2341 caggtcatga acaaggcaat ggcaaatgcg ggtgtaaccc cagagcaaat cgattacgtc
     2401 aacgcgcacg gtacaggtac cgaagccaat gaccgtatcg aaacgctggc aatgaaaaag
     2461 gtcttcgcta accacgataa actgctggtg agctccacca aatcttactt tggccacaac
     2521 attggcgcag cggggattgt ggaactgatt gcctgtctgg caacactgcc ggacaaccgc
     2581 gtactgccga cgctgaattt cagccaggca cgcccgaatt gcgatctgga ctatgtgccg
     2641 aatgaatttc gcgatcgcaa agtgaacatc ttcatgaaga ataactatgc cttcggcggc
     2701 aacaactgct gcatgatcct gagcatggag cccgcgtcca ttccggtcag tacctatgat
     2761 tcgaagcgtg tggctatctc gggcgtcggt gcagtatccg caattggcca cacgctcaac
     2821 caggttctgg aaaacatctg ggcgcaggac caccacgttg aactgggtag cgtaaccttc
     2881 ccggacgata cactggaaga agccaaagag ctgctggccg tgctggatga atcaaaccag
     2941 tttgccgagc tgtttgatga agagtacagc ttcagcgacg ctacgcttcc ggaaacggca
     3001 agcaacttca aaacgttcca ggtttccggg ctggagccgc gtaaacacct gcgtcgtttc
     3061 gatcagcgta aagcaacccg tggcggcacg tttgccctga tcgcactcac cgaagcgctg
     3121 gaacaggcga agcgtaaaat caaacgcgac ggcgatgagc tgggcatgat catggggatg
     3181 tcccgcggcc cgcaggaaac cacctacaaa tatctgcaaa gcctgaaacc cgatccacgc
     3241 aaagtacgta cgtcggaatt ccctggctca ctgatgaatg cgatcccaac gttctgcggg
     3301 atctctgaag ggatcaaagg gtataccact acgcttgcga caggggaaaa ctcggcgctg
     3361 ggcgcactga cctacggtta tgaaatcgtc cggcagaacc tgcaaccgca ggtattggtt
     3421 ggcggcgcgg atgagtactt cccgtcgatg tcgctgtata tggatgcggt gacacaaaag
     3481 atccacatga cggctgaggc gagcgactat caaatttacg gtaaagatcc gcagggttat
     3541 gttccgggcg agggtgcctg catgctgctg ctggaagatc cacagttggc tgtggcgcgt
     3601 ggcgctgagg ttctcgcgga agtggtgggc tacggtaagt catgtagtaa tacctacttc
     3661 gacgcctcat tgttggaaga aaaatccgcg tcgatggcgc tggccattga acgcgccctt
     3721 acgcatgcgg gcatctctgc tggcgatatt gatttggtat gtggtaccag caacggcagc
     3781 gatgaccatt ctcgtatcga aattgacgcc atttacaaca ccttcgccgc gcaaaacccg
     3841 gcggtgccgg tagtgaacta caacgcttgc tttggttttg tcgcctctgc cgctggtttg
     3901 cttaacctcg ccgtgattac tgactgcatt aagaagcagg cagtacctgc gattccaaat
     3961 acggtggaat tctttgatga gcgtgtgaat tttgtacgcc agtcgatgaa acttgcgctg
     4021 aaccatgtcc tgcttgttgg cgcaacggaa ggcggcaact actacgcgtt cgtcattaag
     4081 ggataagact gtggacaatg ccgctattat ttcgggttac agcgtgtgga tgccgtatgc
     4141 cgaagactgt tcgcagttaa ttgacaatct gaaacagggg aaacgcgttg cgcgtacacc
     4201 ctggtttacc tccaacgagg aagccattaa atgcgggttt aagggtaatc cgtcagtggc
     4261 gacgttgaaa caggtggacg atagcgcatt ggacctgctg tctcagttga tcgacgaagc
     4321 cctggaacag gcaatgctgg ataagcattg cctggccggg cgcaacgttc gcgtttacct
     4381 gaccggtatt gggccgcgta tcgacgggct ggattacaaa tctttctata attacaacga
     4441 tgtagaagat atcaacttaa cgcaatccat caccaatctg catgccgcga agatgtcgca
     4501 ggacacgatt tcaagccatc ttgcgcgcaa atatcgcttg cagtatttgc cgcctaacat
     4561 gaactgcacc agtaactcgt cactgactgc ggtgcatctg gcgacgcagg gcattgagca
     4621 gggcgggatt gatctcgcga ttgtgctgaa ctgttcgaaa attaaaaccc aggatatctg
     4681 gttcctggaa actcaatcga tgctggacag cgaacaggtt cagccgtttg gtgaaaacag
     4741 taaaggtgtg gcgttcgcag aaggttttag cgcgctccta ctggaaagcg ctcatcaccg
     4801 ccgggcgcgt caacaaagtg agggcgtaag ggtgcaaacg acttacaccc aaatcagcgc
     4861 gggccgcagc aacgatgcct cctggcttag cactaacgtg cacaaggtca tgcaggccgc
     4921 gatgaagcag gctgaaattg cgctggatga tcttgcagcc attcttccac acggcaacgg
     4981 ttcggcagtg agcgataacg cggaggccaa agccatcgcc atgttcgcag gggagcgaca
     5041 gatccccgtt ctcgcctata aagggcagat cggctatacc gcaaccggat ctggtgttgt
     5101 cgatctgatc attggccacc attcgttgac gcatcatcaa ctgatcgcac ctgttggcaa
     5161 cgacgtcatt atcgacagca tggcttcgct ggtacttacg gacggcagcg tgacaaacca
     5221 tagcaaacgc catttgctga aggttggtgt gggtgtcgat ggttcggtta ttggcgttgt
     5281 tatgtcaaat atgcaagcgg ggcgcgcgaa atgacggaaa agagtatcta cctgtcttcc
     5341 tgggcgctta cggaaccgga cgacattttt tatttttatc agccgacctg gctcaacgcc
     5401 tgggaagcac atatgtacga aaggggatta tacggcaccc gtgctaatgg ctggggactg
     5461 aagcgctttg gcgtggagaa cgatatttat gcgccagaac ggatccgtaa tccacaatat
     5521 atgccttacc ttgaggcgat ggagcaggtg accgattctg cattacaaca aatgccgact
     5581 aaagaacgca tgaagttaat gaaatcgcgc acggcattta tttatgccga ttcctggggc
     5641 gaatccggtt cgtttgaaaa aatttccagt gcgctgcata tcgccactat tgatacgctt
     5701 cccaagaatt tacttaagaa gttttcgata acggaaccga cttgcaagat tcgcggcgaa
     5761 aaacaggcat tcgttcaggc gctgcgtatg gcgcaggatt atctcgcctg ggatgtattc
     5821 gataacgtgg tgatttgtgc tgcctatcgg gcaattccgg tgctggtatt ctctgaagaa
     5881 gatgtgggta aaccgcctaa gcgtttattt ccccgtcgtg ctgatgaggt gaatttatct
     5941 gttgaacgag tgggttgttt tattctcagc cagcgtgaga gcgccatcag gctgcaatgc
     6001 ggggaatatc attccggcac tgcgccacag catgctacgg atattgatgt catggccttc
     6061 agcgaacggc aacaaatgcc gtcccggcct gctgctggtg ttatcgacct ggtggcccat
     6121 tacggtgaaa gcggttgtct gacgcccgcg ttgagtatgg attatttaac ccgtcatatt
     6181 cagcccggtg gaaaaatgcg tacagtcatt gccgataaac agtttggtta tcactacttt
     6241 gaccttgaat accttgaagg ataacgacag tcgtatgcaa tctactatta aatcgttgcc
     6301 cgctatttcg cttagcaaca tttacaagtg ttatggcgct ggggcaggaa aggttgatgc
     6361 gcttaaaagc attaatcttg atatcaataa aggcgaattt ctcgcactgt gtgggccgtc
     6421 aggcagtggt aaaagtactc tgttgaatat tctctcgggt attgataaac cgaccagcgg
     6481 aacggtgata tttttaaata aattactgaa ccaactgccg gaagagcagt tggctgtgat
     6541 tcgtggaaaa catttgggtt ttatttttca gttttttaac cttatgccgg tattaaatat
     6601 tttcgataac gtttatttcc cactggtgct taatggtcag ttcagtaaaa aagaagccac
     6661 tgaacggacg ctgcattatc tcgatagtgt tggattaagc ggttttggcg atcgcaaacc
     6721 ggggcaatta tcgggcggtc aacaacagcg tgtcgccatc gcccgggcac tggcccacga
     6781 accgatggtg gtcattgccg atgaaccgac gggaaattta gacctggcaa ccggcgaggc
     6841 tattttggac ctgctgttga cgattaatca gcagacaggc accacatttg ttatttccac
     6901 gcactccagc gaattaaaag cgcgggcgcg gcgagtggtt gaaattcagg atggagtatt
     6961 gattcatgac tcagatgcat aaaggatttt tgcttatcgg cgtctttttg tacgcggcgt
     7021 ttttggcccc gaagatttac gcggcaactc ccgacgccgt taaccaggtt ctgcaacaag
     7081 ggcttccggc gcagcctgcc gcgctggcga cgctgaccga agcgttaaag aaagagtatg
     7141 aagccaggca caatgtggtg gcgctggttt tttacgccta tggcttactg cgtcaggcgg
     7201 acggttatgc ggcaacaaac gattttatcc atgcctcaga atacgccaaa agcggctttt
     7261 tctggctgga tgaagcggtg gatttgcagg agaaaaatca acgcgtacgc tatttacgtg
     7321 cccgcgtaga tgcctattta cccgcagact cagggcgttg cgtggtgaca gtcaaagaca
     7381 ccgaacacat gctggctgac ccggcgatat ggacgacaac gatccgcgac catattctgg
     7441 cgatgcgtta tcgtgcatta cgtcactgca aggacaccac ccgcgcgaat gcgttgttgg
     7501 cgcaaattaa agggcaaaac gccgcgcttg cgcagtcgct gacgcaggat tttaatgttg
     7561 taccagaatg ggatagtgaa gagttaaccc aggtactgct gccgctgatg aaaggtgagt
     7621 aaatgtccgg ggaaatttta gctgttaata gcgttctgct gctgattctg ttttttgtgt
     7681 tttatcagct tctgttttat tcacgagatg tcaaatttgc atttttgaat ctctttcgcc
     7741 acaagcgtcg ctctttttct actataaccg ccattgtatt gggtggcgtg gcaatttttt
     7801 tatatggcgg atttattgat tactctttct ggatcctgaa agagcagacg attcgtacca
     7861 atattggtca tgtacaaatt tacaatcagc actattttga aacgtcgaat aaaaataaga
     7921 gcctgattgc cgattatgcg gcgttaaaga aagctatttt gtcggacccg acgctggcga
     7981 cggatatttc cacgctctcc gggcaactgg agtttactgg cgtaatttca cattacgaga
     8041 gcgaaacctc cagctatttc tctgcgctgg gggtggagcc gttacccgct ttaaagctgg
     8101 gttcgttcga taagattatc gcggggagcg atctttcccg ggtaaaaggt gatgaaatca
     8161 ccctgggcag cggtctggcg aaaacgttga atgcgaaata tgacgactgg ctggatgtga
     8221 tggtggtcaa cacggcgggc gggcagggcg cattgtcgct aaaaatgcgt ggcatttttg
     8281 agtcaggcat taaagattat gacgatgtcg cgatgaagat cccgctggac accgcgcagc
     8341 gcatgatggg tacggatggc gtcagcaaag tgcttattct gcttaaagaa gatggcaccg
     8401 cggcgtttag cgctaagctg cgtcagttta tcgaacgcaa tcatttgccg ctggtggtca
     8461 aagactggcg tgatgtttca ctcttttatc aacaggttga agggttgcta tcggggatct
     8521 atttcttcat caagctgatt gttgcgctga ttgtcatctt catgatcggc aattcgatgg
     8581 caatgaacat tgttgagcgc acccgtgaaa taaccactct gcgtgccata ggcctgaagc
     8641 cgcttcacgt cacgcgcttg tttctgacag aaggcatttt tattggtgtg attggcgccg
     8701 tcggcagcct gctggtgggg ggcgtgttag cctggattat taatctttat ggtattgcga
     8761 tgccgccatc gccgggacaa accatcggct ataccgcctt tataaaaacc agtaacccgg
     8821 aattgatttg ggtgacggtg gtattaccta ttttgacagc aacaggtgcc tctgtgttac
     8881 cggctttacg tgcttcccgg ctgaatattt ctgacgcatt taaattctcg taggactgat
     8941 tatgaatatg gctttttacg gcaagtggtt tgcttgcctg tggctcgcta cgtcgtgcgt
     9001 tcaggcggcg agcacagatg ataaggcgct ggagataatt cgtcgcgctg acgaaattcg
     9061 ttcgccgaat aaaccgtttc gttacacgct gacggtaacg gaatataaag cgggcgcaac
     9121 ccagccagag aataaacagg tcctcgatat ttccatgcgc tttatgaaac cgcagggtaa
     9181 tgaaaaagcg gatgcacgct cgctggtgcg ttttatttat ccgccgcgag ataaaggaaa
     9241 agtcatgctt tccgactggt acgatttatg gttttacacg ccggaactgc gacgtccgat
     9301 gcctatttca cggcaacaaa gattaattgg gcaaatctct aacggcgatg tcattgtcac
     9361 taactttgaa tatgcctatg actcgaccct tatgggggaa gtcccgtgtg ctgaaaagca
     9421 gtgttacaag ctggcgctgg tgcgtaaatc ggcggatatc acctggccga aggttatcta
     9481 ttacgttgaa aaagacggtg ataatcgccc gtggaaggca gcctattact cgcaggatga
     9541 ccagttaata aaagaagtgt tgtatcagga tttccagccg gtgctgggga aaacgcgacc
     9601 catgaaaata accgttaccg atgttcgtca cggcaataac tattcagtga tggagtacag
     9661 cgatgttcgt ctggaatcgc tgccagagtt tcatttcacc aaagagtata ttcagcgagg
     9721 tgcgaaatga aacgcacgct gaagatctct tcgttgctgt gtgttgcgct gccgttgacg
     9781 gtacaggcgg attgcctgtc aggtgacgag gtggcacaaa acagcgatgt gacgctttac
     9841 gagcaggtgt cttatgtcaa taaacaggct tcggcctggc agattgcagg caaaaatccc
     9901 tacactcgtc acaacggtta tcaggaggcg gggattggca tcaacagtgg ttgttcgata
     9961 atcgacaata cgctggacct taagctcaat ctgtatggaa taaacgaata tgcgcttaaa
    10021 cccgcaggga aattcgaaac ggatgatagc cgcacacgtg cgttaattaa ccgcctcagt
    10081 ttggtctata gcgcctcgga cagcgttcaa tttgaagcag gcaaatttgc cgcgccgtca
    10141 ggaatgttct ttttgcgttc gccttctgat ttgcaaacgc attattacac cggcttccag
    10201 tcgacgcgtt tgcacgatcc caaaatgaca tcggcgtatc aggcgtcatc gtggggtgca
    10261 aaaatgagcg tggacacacg cgattatgcg ttttcggcga gcgtcatacc gaagttagcc
    10321 accatcgaca aacgctatgt gacgtctggc aactggtctg ctaatcagca gggaaacagc
    10381 gacgaagcct atctgctgag ctacagcgat catcgctttg gtgagcacac accgacagtc
    10441 aatgtgcggc tcgggccatc gccgtcactc gcgctgtctg acagttatca ctacaccccg
    10501 cagctgacgc tgaatgtcga cgcagcctac caccgctccc aacaatggcg gcatctttcc
    10561 caccatgaga cggcgcaggt cgaggaatat cagtttccgg attcgctcta tgaaacgaaa
    10621 gacgaaagcg gcgtggagct ggccctgggg gggcagtaca ccagcgatag tttcagcgtg
    10681 tttggcgttg agtattactt tcagagcgag ggttattcgc gagctgaaca gcgtcagcag
    10741 cgcgagttaa ttgatttcct caacaccaca accggttatg cgccactgga tcaggcattt
    10801 gattcttaca aatacctgat ggcttcagaa atcagcaata ccgccaatca ggggatgctg
    10861 cagggtaagc actatctgaa cgcctgggcc agtctgccgc tagcgggaga gtcgacgctg
    10921 cagccttcgc tggtggtgaa tctcgttgat ggcagcacgt tgctgggcct gcattattcc
    10981 acgccgctca gcgctataag taaccagctt gaagcctatg ccggtggcta tagcgcgctg
    11041 ggaagtcggt attctgaatt tgcgcttttt ggcgacacgt taggccttta tttgggcttt
    11101 aaatactatc tataaggata aagagatgat gaataatgct tcacaaccga cagtactgct
    11161 ttttgccggt cagggtaatc cggttattgg catgggcagc gatctttggg atctcaacgc
    11221 cagtacaaaa catatctggg attgtgccag cgatatttcc ggactgaacc tgcgtcgcct
    11281 gtgtcagaaa gggccgatga atcgccttat tcaaacgacg gtacaacaac tggcggtgac
    11341 cgcaattaat gtaacgctct ataccctgtg ccgtgaacgt cttgagaatt taacggttgt
    11401 ggggtcctgc ggacacagcg ttggtgaata cagtgcgctc tacgctgccg gagctatttc
    11461 tcttgagacg ctatttggca tgattcactt cagagccagc ctgatgcatg acgaaagcca
    11521 gagaaataaa ggcgcaatgc tggcggttaa aggcatcaac cgcgaacaac tgcagacgat
    11581 gattacggaa tcaggcattg cgctggatat tagctgtgat aacacgccgc gccagcaggt
    11641 tattggggga acgcaggctg cgttgaatga atttgccacg ctgctgatgg ccgctggata
    11701 cgaaccggtt aaactcggtg tgagcggtgc ctggcacacg cgtttgatgg aagacggcgt
    11761 tcaggcaatg cgcgattatc ttgccggact ggatatcgct tcgccagagc atcaggttct
    11821 gatgaacgta acagcaaaaa gcgaggtcgc tccgtcaatt atcaaagaaa atctctcatt
    11881 acacttaact catacggtga aatggactga atctctcgat acatttctga atatgccaac
    11941 ctcggtagcc ttccttgaaa taagtaataa gccctatttg gggaatatgt taaacgattt
    12001 tgctggtgtg gatcagcaac gagttatgca ttgtcgtaaa gcatttactg atgcgaaggt
    12061 attcaaatga ttcctgatta tttaactttt attcgctttc aggataaacg aagtctgata
    12121 tacatttatg ctattggact tattctgata ggtttttatt ggaaaaatgc aggatttact
    12181 tttccatcag aggatattgg tgtcgttagt gggattctgg ccctggtgct gtataatttt
    12241 atttttgatc tcaaggcgta ctgggcttat aaatgcgtca cgaagaatat cgatttttcg
    12301 tggtttaaga aaaagcagaa ccacaaaata gaattatttc ttacgcaacc tctggtggca
    12361 ggatttctgt cgttaatcat gttgagtgca atgagttggg ggctatacca gcgtctaccc
    12421 tcgttatatg cgctgttcct gatttcgtta cttgggccgt tggttatctt tctgttgttt
    12481 cggatgatcc gcaccagtta tgtcaagcag gtcgctattt cagtggcgaa aaaagtaaaa
    12541 tataaaagtc tgactcgcta tgtgctgctt tcggtgtgca tctcaacggt tgttaacttg
    12601 cttactatca gcccgttgcg taacagtgat tcttttgtga tagaggggca gtggttaacg
    12661 tttaaatcga taattgcatt gctcattctt tgtggcgtag tgttggcgat taatctgttt
    12721 tttctgcgct tctccaggcg gtacgctttt ttgggcaggc tttttttgca ggaaatcgat
    12781 ctctttttct ccagtgaaaa tgcgttgtcg accttttttg ccaatccgct ttggcttcgg
    12841 ttattcatat tgctggttat tgaagtgatg tggattacgc tggtgtcggt attggcaacg
    12901 cttgtggaat ggcggatttg gtttgaagcc tattttttac tctgctatgt accgtgctta
    12961 atttactatt ttttctattg tcgattcctc tggcataacg attttatgat ggcatgtgac
    13021 atgtatttcc gttgggggca ttttaataag tgaggaagtg ataggaagtg accagataat
    13081 acatatatgt tctgtactct cttgcgcatt ttgattgttg actgagcaac cagacagttg
    13141 atgtgcacga tttcccctcg ccctagcaga cgtgggcggg ggcacccggt gtgtgcaata
    13201 tacgaaaaaa aagcccgtac tttcgtacga gctcttcttt aaatatggcg gtgagggggg
    13261 gattcgaacc cccgatacgt tgccgtatac acactttcca ggcgtgctcc ttcagccact
    13321 cggacacctc accaaattgt cgttcctgtc ttgctggaac gggcgctaat ttagggaaat
    13381 catgacctga ggtcaacaaa cttttttaaa aaatcgcgcg tttattcaaa cttcaatcaa
    13441 tatgtggttt taataagcga aatctgcttt ttttgccacc gaccacggat ttgttatgct
    13501 ggtggtcttt gtagatcata acgataagtg cgaataaatt tcgcacaacg cttttcggga
    13561 gtcagtatgg atatcatctt ttatcaccca acgttcgata cccaatggtg gattgaggca
    13621 ctgcgcaaag ctattcctca ggcaagagtc agagcatgga aaagcggaga taatgactct
    13681 gctgattatg ctttagtctg gcatcctcct gttgaaatgc tggcagggcg cgatcttaaa
    13741 gcggtgttcg cactcggggc cggtgttgat tctattttga gcaagctaca ggcacaccct
    13801 gaaatgctga acccttctgt tccacttttt cgcctggaag ataccggtat gggcgagcaa
    13861 atgcaggaat atgctgtcag tcaggtgctg cattggtttc gacgttttga cgattatcgc
    13921 atccagcaaa atagttcgca ttggcaaccg ctgcctgaat atcatcggga agattttacc
    13981 atcggcattt tgggcgcagg cgtgctgggc agtaaagttg ctcagagtct acaaacctgg
    14041 cgctttccgc tgcgttgctg gagtcgaacc cgtaaatcgt ggcctggcgt gcaaagcttt
    14101 gccggacggg aagaactgtc tgcatttctg agccaatgtc gggtattgat taatttgtta
    14161 ccgaataccc ctgaaaccgt cggcattatt aatcaacaat tactcgaaaa attaccggat
    14221 ggcgcgtatc tcctcaacct ggcgcgtggt gttcatgttg tggaagatga cctgctcgcg
    14281 gcgctggata gcgggaaagt taaaggcgca atgctggatg tttttaatcg tgaaccctta
    14341 ccgcctgaaa gtccgctctg gcaacatcca cgcgtgacga taacaccaca tgtcgccgcg
    14401 attacccgtc ccgctgaagc tgtggagtac atttctcgca ctattgccca gctcgaaaaa
    14461 ggggagaggg tctgcggtca agtcgaccgc gcacgcggct actaataaag catcaggatt
    14521 cctgctatcc ttggcgggaa ttgaatacag gagagagtta tgtatcccgt cgaccttcat
    14581 atgcataccg ttgccagcac acatgcatat agcacattga gtgattacat tgcccaggcc
    14641 aaacaaaagg gcattaaact ttttgcgatc accgatcatg gcccggatat ggaagatgcg
    14701 ccgcatcact ggcacttcat taacatgcgt atctggccgc gagtggttga tggggtaggg
    14761 atcctgcgcg gcatcgaagc taacattaaa aatgttgatg gtgaaattga ttgcagcggt
    14821 aaaatgtttg actcgctgga tctgattatt gccggttttc atgagccggt ttttgcacca
    14881 catgacaaag cgaccaatac acaagcgatg atcgccacta tcgccagcgg caatgtgcat
    14941 ataataagcc atcccggaaa tcctaaatat gagatagatg ttaaagccgt tgctgaagca
    15001 gccgcgaaac atcaggtggc gctggaaatc aataattcct catttttaca ctcacgtaag
    15061 ggcagtgaag acaactgtcg tgcggtagcc gcagcggtac gtgatgctgg aggttgggtg
    15121 gcattaggct cggattctca caccgcgttt accatggggg aatttgaaga gtgtcttaaa
    15181 atcctcgacg cggtagattt tccgccagag cgcattttga atgtttctcc gcgccgctta
    15241 ctgaacttcc ttgaatctcg cggtatggca ccgattgcgg aatttgcaga cctttaatta
    15301 ctagtaatgg aaatatatta atgaacgagt tttctatcct ctgtcgtgtg ctgggttcgc
    15361 tctattaccg ccaaccacaa gatcctttac tggtgccgct gtttaccctg attcgtgagg
    15421 ggaaactggc tgcgaactgg ccactggagc aggatgagtt gctgacacgt ttgcagaaaa
    15481 gctgtgatat gacgcaagtc tctgccgatt acaatgcgtt gtttatcggc gatgaatgtg
    15541 ccgtgccgcc atatcgtagc gcatgggttg aggacgcaac ggaagcggaa gtgcgcgctt
    15601 ttctttctga acgagggatg ccattagcgg atacgcctgc cgatcacatc ggcacattgc
    15661 tgcttgcggc ttcctggctg gaagatcagt caacggaaga tgagagcgaa gcactggaaa
    15721 cactgttcag tgagtatctg ttaccctggt gtggcgcgtt tcttggcaaa gtggaggccc
    15781 atgcaaccac gcctttctgg cgcaccatgg caccgctaac ccgcgatgcc attagtgcaa
    15841 tgtgggacga gctggaagaa gattctgaag agtaaatgtg atctacgtca ctcataactg
    15901 caacgtataa tttgttgttg cataaaatgt gtgctcgatc tcattcatgg ccgcgttttc
    15961 tgctatcatg cgcggcatga acatacttct ctctattgca atcacaacag gcattctctc
    16021 cggtatctgg ggatgggtgg ctgtttccct gggcttacta agctgggcgg gcttcctggg
    16081 ctgtacggcc tactttgctt gcccgcaagg tgggctgaaa gggctggcga tctccgctgc
    16141 aaccctgctt agtggcgtgg tgtgggcgat ggtcattatt tacggtagtg cactggcacc
    16201 acatctggaa attctcggtt atgtcataac cggtattgtc gcgtttctga tgtgtattca
    16261 ggccaaacag ctgctgcttt catttgttcc gggaacgttt ataggcgcat gcgcgacgtt
    16321 tgccgggcag ggtgactgga aactggtgtt accttcgctg gcgcttgggc tgatatttgg
    16381 ttacgcaatg aaaaacagcg gcctgtggct ggcggcgcgt agtgcaaaga cggcgcaccg
    16441 tgagcaggaa atcaaaaata aagcgtgagg ggcactcacg cttttgctta aacagtaaat
    16501 gccggattat taattctggc atttttatct gtcaggattc cggtggaacc gacatatggc
    16561 ggtatttcac cagaatgtca ttctgccgtt ctgctttatt ttgcaaatcc cacagaccac
    16621 ggtcgatacc atcattaatc aggaaaatga cccctgtttc gatagccgac atcaggcaca
    16681 gcataacagg ttcgttcgag gtgtaaccca cttccccttc cagcaagcgc tggtagtcaa
    16741 taaagcggaa aaccccggcc tgaacttcat aggaaagtat cgtcttactg gtgttcaccg
    16801 aggagaggat ctcaccggta ctcacattga cgacgcgcag gttcacggca atctgatcga
    16861 gctggtattg cgtgtcggca ccgatcccaa aatatcttgc cccaaccccg cccgatttga
    16921 cgttgctttc ataaccgata atcgaacctt caaccatgat atttgccgcc gttaaagatt
    16981 gcagcgggat tcggttatta atggcaaccg tgccgttttc ttgtgccgca cgaataatct
    17041 tgcgctcgtt aagcaggttt tgtaagccct ggcgctccag cggtataaac cagcgagaat
    17101 ctttcaatgc cgtgaccagc atcgccgtgg cgctttgcgg aacagcagtg gagaagttac
    17161 ttgccgggta gggtttaaat tgcccggttt cgtcctgaat gttgtatacc gaaacaaaga
    17221 tcttacccgt cggcgctggc agatgggtca aatctttgta gctctgagca cgaggcatta
    17281 atgtcggtct ggcggcttct ttaggcgggg cggttaagca tccgctcagt aacatgacgg
    17341 caaccaaaag aaataagcgc tgcatgatta ttttccttat gaagctgggg cttaaaaatc
    17401 ggttgagtta ttttgtaaac ccgaaacctg gatggtcgag gtttgtccgg ttttacgatc
    17461 tgtcacgttc aactgcaatt gaccatcgcg gttagcaata tcaacaatat aatcgttggt
    17521 caccatgcgg cccggtttac cggtattaat attcgacagt agcccaccta aaatttgtga
    17581 ctggatggcc tgagtaaagt tatctaacgc tgagggtgtt tcaataccaa agtcatcgtt
    17641 atagctcgga tctttataag agttttgggc ctgagcgcta tttaataaaa aagcgccatt
    17701 atttgggtta ccaccaaagt ttggattacg gaactggaaa gtcatggttc cagcccaact
    17761 taatggcgaa ataagcatga gtagaactac tgcatgtttg acacgcattg cagcctccga
    17821 acaatttttt atttagaatt catcatgcgc caaatcgccc gtacttaata acgcctgatt
    17881 tatctggcga cgatttagtg cttcttcagt ttgaatcagt gcaaagacga cagttttttc
    17941 gaagtctctt ttcaacggga ataaaaaagt ctggaaaata acgtcctgat tcaccgttat
    18001 agtgatccag cttccccatc gtgcactggg cctttcatta atcgttaagt tacccgtata
    18061 gtcactttcc catttatcac taaaggctcg gtaaaaatca tggccaatag atgaaacagt
    18121 atggtcagtt aacaatcccg ggacttctac ctcaacggcg tgaagattcc ctgcggcgaa
    18181 cagaaattct gccgccacaa tccagcgtaa ataacgtttc atggctttat cgcctgaggt
    18241 tatcgtttgc ccaggaaacc gcttgtgtcc ggttttttac ggctatcttc ttgaaaagat
    18301 tataaagatg cgttttaacc gtattttcgc tgatgaacaa cgaacgagcg atctcgttat
    18361 tagacgcgcc gatacgcagc ttattcagga tctctttttc ccgatgagta aggagggctg
    18421 attccgtgct gttataacga tagttaccag aatgtgtaat caggtaactg gcaagctttt
    18481 gcgtaaagta gcattcgccg cgcaggacgc cttgcaaccc attgacaaca cgttcttgat
    18541 cctccatggc ataaaaaacg ccgttgatat gaggccagtt ttcaatgtcg cggtacgggt
    18601 aatcttcagg cgtatttagc aacaatattt tgatattgtt gtttttcctg ctcaaagtat
    18661 cctgccaata atggataagc tttttatccg cttccatcat atccagaaga ataatagagc
    18721 ctgaagagat atcgtccaga gaacgttgaa tattatgtaa ttttcctgta attgccagcg
    18781 attgtttaag gtgctgcaag agagctgtcg cctgcaaaga aggtttagtg atcaacaata
    18841 atgtatgacc atgaatacta tggacttcat taaacatgat gaaaccccgc tttttttatt
    18901 gatcgcacac ctgacagctg cctctaaaat agaagcacca gaagtactga cagatgttgc
    18961 actgctgtgt gtagtaataa atcagcccta aatgggtaaa atataaaact aatggattac
    19021 atctgatttc aatctagcca tta
//
LOCUS       DAZUCE010000083        18936 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZUCE010000083 DAZUCE010000000
VERSION     DAZUCE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.083"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            166..378
                     /gene="cspH"
                     /locus_tag="ACHY03_002450"
     CDS             166..378
                     /gene="cspH"
                     /locus_tag="ACHY03_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFS3905666.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            820..1125
                     /locus_tag="ACHY03_002451"
     CDS             820..1125
                     /locus_tag="ACHY03_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905667.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1232..1876
                     /gene="gfcB"
                     /locus_tag="ACHY03_002452"
     CDS             1232..1876
                     /gene="gfcB"
                     /locus_tag="ACHY03_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFS3905668.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1873..2619
                     /locus_tag="ACHY03_002453"
     CDS             1873..2619
                     /locus_tag="ACHY03_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFS3905669.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2619..4715
                     /locus_tag="ACHY03_002454"
     CDS             2619..4715
                     /locus_tag="ACHY03_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFS3905670.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4761..5900
                     /locus_tag="ACHY03_002455"
     CDS             4761..5900
                     /locus_tag="ACHY03_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFS3905671.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5888..6334
                     /gene="etp"
                     /locus_tag="ACHY03_002456"
                     /gene_synonym="yccY"
     CDS             5888..6334
                     /gene="etp"
                     /locus_tag="ACHY03_002456"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFS3905672.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6354..8534
                     /gene="etk"
                     /locus_tag="ACHY03_002457"
     CDS             6354..8534
                     /gene="etk"
                     /locus_tag="ACHY03_002457"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFS3905673.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNVLMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8654..9952)
                     /gene="appA"
                     /locus_tag="ACHY03_002458"
     CDS             complement(8654..9952)
                     /gene="appA"
                     /locus_tag="ACHY03_002458"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFS3905674.1"
                     /translation="MKAILIPFLSLLIPLTPQSTFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVTDGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQVDTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALTPHSPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(10032..10124)
                     /gene="cbdX"
                     /locus_tag="ACHY03_002459"
     CDS             complement(10032..10124)
                     /gene="cbdX"
                     /locus_tag="ACHY03_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFS3905675.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10137..11273)
                     /gene="appB"
                     /locus_tag="ACHY03_002460"
     CDS             complement(10137..11273)
                     /gene="appB"
                     /locus_tag="ACHY03_002460"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFS3905676.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11285..12829)
                     /gene="appC"
                     /locus_tag="ACHY03_002461"
     CDS             complement(11285..12829)
                     /gene="appC"
                     /locus_tag="ACHY03_002461"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFS3905677.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12963..13820)
                     /locus_tag="ACHY03_002462"
     CDS             complement(12963..13820)
                     /locus_tag="ACHY03_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFS3905678.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13817..14215)
                     /gene="hyaE"
                     /locus_tag="ACHY03_002463"
     CDS             complement(13817..14215)
                     /gene="hyaE"
                     /locus_tag="ACHY03_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFS3905679.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(14212..14799)
                     /gene="hyaD"
                     /locus_tag="ACHY03_002464"
     CDS             complement(14212..14799)
                     /gene="hyaD"
                     /locus_tag="ACHY03_002464"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFS3905680.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            complement(14796..15503)
                     /gene="hyaC"
                     /locus_tag="ACHY03_002465"
     CDS             complement(14796..15503)
                     /gene="hyaC"
                     /locus_tag="ACHY03_002465"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFS3905681.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(15522..17315)
                     /gene="hyaB"
                     /locus_tag="ACHY03_002466"
     CDS             complement(15522..17315)
                     /gene="hyaB"
                     /locus_tag="ACHY03_002466"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFS3905682.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIILATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17312..18430)
                     /gene="hyaA"
                     /locus_tag="ACHY03_002467"
     CDS             complement(17312..18430)
                     /gene="hyaA"
                     /locus_tag="ACHY03_002467"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFS3905683.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAIDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4653 a         5001 c         4893 g         4389 t
ORIGIN      
        1 aaactacttt gtatgctgtc tgtctttcaa accgacgcag ctattaacgc atgataattg
       61 agattaagca aattttattt tagccgtccg gcggtcttaa atatcgctgg ctttatatta
      121 ttaaccccgt atagtgcagc gcagtatttt cagtaaggaa tttgtttgtc ccgtaaaatg
      181 acaggaattg tcaaaacctt tgatcgcaaa agcggcaaag gattcattat cccctccgac
      241 ggtcgtaaag aagtccaggt ccatatttcc gcattcactc cccgcgacgc agaagtgctt
      301 ataccaggat tacgcgtgga attttgccgc gtaaatggcc tgcgaggacc aacagcggca
      361 aatgtttatc tctcttaata tgacgctgct tgtttaaagc tgactggttt tattggcata
      421 gttcaatagt caacaccata tattataaca caagttactt tttcaccgga aaactaatcc
      481 atttaaccga atatctctac aactaatagt tataaatgcc acttaacagc gagttatagt
      541 accacttgaa actatattgc caacaatagt taatagcttt cttaacaatt ttcttatctt
      601 taatttgtat tttgctaaga tttatcttat gctcacaagt cgtatttcca gagggaaatc
      661 gttaacaatc ttatatttcc ctctgcgcgc ctttctcacc cgctgtatat agtgcaagcg
      721 gtatgaggaa agggagattt cacaaggatg ttatagtcaa agtagacaat cctgatctca
      781 agcgtacgta ttgtccatgc gatgaataaa ggaaaagtta tgaaacacaa gctttctgca
      841 atccttatgg ccttcatgtt gacgacgcca gctgcgtttg ctgcccctga agcagcaaac
      901 gggaccgagg caacgacggg tactactggc accacaacga ccactaccgg tgcaaccacg
      961 acggctgcta ccactggtgg cgtggctgct ggcgctgtcg gcacggcgac agttgtcggt
     1021 gtggcaacgg ctgttggtgt ggcgacgttg gcagtagttg cagcaaatga cagtggcgat
     1081 ggcggttctc ataacacctc aaccaccacg tccactacgc gctaattctg cttaaacgcg
     1141 tcgaaattaa agttaaataa tacccggttg cgatattcgt accgggcttt agagatttgc
     1201 atttttttaa ttaacctttc aaggattaaa ggtgcgccct cttattttat cgattttcgc
     1261 actatttctt gcgggatgta cgcacagcca gcaaagtatg gtcgatacat ttcgcgccag
     1321 ccttttcgat aatcaggata tcaccgtagc ggatcagcag atccaggcgt tgccttattc
     1381 cacgatgtat ttacgcctta atgaagggca acgaatcttt gtggtactgg gatatataga
     1441 acaagaacaa agcaaatggt tatcccagga taacgccatg ctggttaccc acaatggacg
     1501 tcttttaaag accgtcaaac ttaataataa tctgctggaa gtgacaaatt ccgggcagga
     1561 ccctctgcgt aacgcgctgg caataaaaga cggcagccgc tggacgcgcg atattctctg
     1621 gagtgaagac aaccattttc gctctgcgac cctgagttcc actttttcct ttgctggatt
     1681 agagacgctg catattgcgg gtcgcgatgt gctgtgtaat gtctggcagg aagaggtgac
     1741 ttccacgctg ccagaaaaac agtggcaaaa cacattctgg gtcgattcgg ctactggcca
     1801 ggttcgtcaa agtcggcaaa tgttaggcgc aggggttatt cccgtagaaa tgacgtttct
     1861 taaacccgca ccatgaataa attacagtcg tatttcattg ccagcgtact ttacgtaatg
     1921 acaccccatg cctttgcgca aggaacggtg actatttatc tgcctggcga acaacagaca
     1981 ctttccgttg gccccgtgga aaatgttgcc cagttggtga cacaaccgca actgcgcgat
     2041 cgcctctggt ggcctggtgc cttgctgacc gactctgcgg cgaaagctaa agcgcttaaa
     2101 gactaccaac atgtcatggc acagcttgct tcctgggaag cagaagctga cgacgatgtg
     2161 gcggcaacga ttaaatccgt gcgccagcag ttactcaatc tcaatatcac tggtcgctta
     2221 ccggtcaaac tggaccctga ttttgtacga gtggatgaga acagcaatcc cccgctggtc
     2281 ggggactata cgctctatac cgtacagcgt ccggtgacta ttactctgct gggtgcggtt
     2341 tctggggctg ggcaattgcc ctggcaggca ggccgtagcg ttaccgatta tctgcaagat
     2401 cacccccgcc ttgcaggcgc ggacaagaat aacgttatgg tgattacgcc tgagggtgag
     2461 actgtcgtcg cccctgttgc cctgtggaat aaaagacatg tggaaccacc gcctggcagc
     2521 cagctttggt tgggcttttc tgcgcatgta ctgcccgaaa aatatgctga tttaaatgac
     2581 cagattgtgt ctgttttaac gcaacgtgtt cctgactgat gaagaagaat tcttatcttt
     2641 taagctgcct ggccattgcc gtctccagtg cctgtcatgc tgaagtatta acctatccgg
     2701 atccgctggg ttcgtcgcaa tcagactttg gcggcacagg attgttgcag atgccaaatg
     2761 cgcgcatcgc accggaaggt gaattcagcg tcaactaccg ggataacgat cagtaccgtt
     2821 tctactccac gtccgtggcg ctgttcccat ggctggaagg caccattcgt tatacggatg
     2881 tgcgcacacg caaatatagc cagtgggaag atttcagcgg cgatcagtca tacaaagaca
     2941 aatcattcga ttttaaactt cgcctgtggg aagaaggtta ctggctaccg caagtggcgt
     3001 ttggtaaacg tgatattgct ggtacgggtc tgtttgacgg tgagtatctg gtggccagca
     3061 agcaagcggg gccatttgat ttcaccctcg ggatggcatg gggctacgcc ggtaatgcgg
     3121 gcaatattac caacccgttt tgccgggtga gcgataaata ttgtcatcgc gcagagtctc
     3181 acgatgcggg cgatatcagc tttagcgata tctttcgtgg cccggcttcc atctttggcg
     3241 gcattgagta tcaaacgccg tggaatcccc tgcgtttgaa actcgaatac gacggcaaca
     3301 attaccagaa tgatttcgct ggcaaactgc ctcaagcaag ccatttcaac gtcggtgcag
     3361 tttatcgcgc tgccagctgg gcagacctca acctgagtta tgaacgcggt aacacgttga
     3421 tgtttggttt cactttacgg accaatttca acgatctgcg ccctgccctg cgcgatacgc
     3481 caaaaccggc atatcaacct gcgcctgaat ctgaaggatt gcagtacacc acggtagcaa
     3541 accaacttac cgccctgaag tataacgcgg gctttgacgc gccagaaatt cagctacgcg
     3601 ataagacgct gtatatgtct ggtcagcaat acaaataccg tgactctcgt gaagcggtcg
     3661 atcgtgccaa ccggattctg gtgaataacc tgccgcaagg cgttgagaag attagcgtga
     3721 cgcaaaagcg cgagcatatg gcgatggtga ctaccgaaac cgacgtagcc agcctgcgca
     3781 aacagctggc tggtacagcg cctggtaaat cagagcaact gcaacaacaa cgtgttgaag
     3841 cagaagatct ttctgccttt ggtcggggct accgtattcg tgaagatcgc tttagctact
     3901 ctttcaaccc aacactttca cagtcgctgg gcggcccgga agatttctat atgttccagt
     3961 tggggctgat gtccagcgcc cgctactggt ttactgacca cctgctgctt gatggcggta
     4021 ttttcaccaa tatttacaat aactacgaca agtttaagtc ttcgctatta cccgcggact
     4081 ctaccctgcc ccgcgtgcgc acgcatatcc gtgattacgt tcgcaatgac gtttatctca
     4141 acaacttgca ggcgaactac tttgccgact taggcaatgg tttctatggc caggtgtatg
     4201 gcggttatct ggaaacgatg tacgccggtg tcggttccga gctgctttat cgcccgctag
     4261 atgccagctg ggcgctgggt gtggacgtta actacgtgaa gcagcgtgac tgggacaaca
     4321 tgatgcgctt caccgattat tccacgccaa ctggtttcgt gacggcttac tggaacccgc
     4381 cgacgctcaa tggcgtactg atgaaactta gcgttgggca atatctggca aaagataaag
     4441 gggcaacgat cgacgtcgcc aaacgctttg acagcggcgt ggcggtaggg gtatgggcgg
     4501 caatcagtaa cgtatctaaa gatgactacg gcgaaggcgg ctttagtaaa ggtttttata
     4561 tctcgattcc gttcgacttg atgaccattg gacctaaccg caaccgcgcg gtggtttcgt
     4621 ggacaccatt gacgcgtgat ggtggacaaa tgctgtcacg caaataccag ctctatccaa
     4681 tgacggcaga gcgagaagta ccggttggac aataacctga cgtaattcat aacattacat
     4741 tgactgataa atataaacca atgaaaaaga acatttttaa gttttcggta ctgacattgg
     4801 ctgttctgtc tttgacagca tgtacccttg ttcctggtca gaatttatcg accagtaaca
     4861 aggatgtcat tgaactacct gacaatcagt atgacctgga caaaatggtc aatatctatc
     4921 ccgtcacgcc aggattgatt gatcagttgc gcgccaaacc gattatgtcg caggctaacc
     4981 cggagctgga gcagcaaatc gcgaactatg aatatcgtat cggcattggt gatgtgttga
     5041 tggttacggt ctgggatcac ccggaactaa ccacccccgc agggcaatac cgtagcgcca
     5101 gtgataccgg caactgggtt aacgccgatg gtgcgatttt ctacccctat attggtcgcc
     5161 tgaaagtggc cgggaaaaca cttacccagg tacgcaacga gattacagcg cgtctggatt
     5221 cagtcattga aagcccgcaa gtcgatgtca gcgtcgcggc gttccgctcg caaaaagcgt
     5281 acgtcaccgg tgaagtcagc aaatcgggtc agcagccgat taccaatatt cctctgacca
     5341 tcatggatgc gattaacgcc gcgggcggtc tgaccgcaga tgcagactgg cgcaatgtgg
     5401 tgctcaccca gaatggcgta aagaccaaag tgaacctcta tgcgctgatg cagcgtggcg
     5461 acttgcgcca gaacaaactg ctgcacccgg gagatatttt atttattccg cgcaacgatg
     5521 atctgaaagt cttcgtgatg ggtgaagtgg gtaaacagag cacgctgaaa atggaccgca
     5581 gcggtatgac cctggcggaa gcgctgggta acgctgaggg tatgaatcag gatgtcgctg
     5641 atgcgacggg gatttttgtt attcgtgcaa cgcaaaacaa gcagaatggc aaaatcgcca
     5701 atatttatca gctgaatgcg aaggacgcat cggcaatgat actcggaacg gaattccagc
     5761 ttgaacctta cgacattgtt tatgtcacta ccgcgcctct ggctcgttgg aaccgtgtga
     5821 tttccctgct ggtaccgacg atttccgggg tgcatgacct gacagagact tcacggtgga
     5881 ttcaaacatg gcccaactaa aatttaactc aatcctggtg gtttgtaccg gcaacatttg
     5941 ccgttcgccc attggcgagc gcttgctgcg taaacgtctg ccgggcgtaa aagtcaaatc
     6001 ggcaggtgtt catggtctgg taaaacaccc tgcagatgcg acagcggcag atgttgccgc
     6061 taatcatggc gtatctcttg aaggacatgc cggacgtaag ctcactgctg agatggcacg
     6121 aaattacgat ctgattctgg caatggagtc ggaacatatt gctcaggtta ccgcaatcgc
     6181 tcccgaggtc cgcgggaaga caatgctgtt tgggcaatgg ctggaacaga aagagatccc
     6241 ggatccctat cgtaaaagtc aggatgcatt tgaacatgtc tacggaatgt tggagcgcgc
     6301 cagtcaggaa tgggcgaagc gcctcagccg gtaatttgag tttataaaat acgatgacaa
     6361 ctaaaaatat gaatacgcca ccaggcagca ctcaggaaaa tgagatcgat ctgcttcgtc
     6421 tggtcggcga gttatgggat caccgtaagt ttattatcag cgtgaccgcg ttattcacgc
     6481 tgatcgctgt cgcttactcg ctgttaagca caccaattta tcaggcagat actctggtcc
     6541 aggttgagca gaaacagggc aacgccattc tcagcggcct gagcgatatg atccctaact
     6601 catcgcccga gtctgcaccg gagatccaac tgctgcaatc gcgcatgatt ctcggtaaaa
     6661 ccattgctga actgaatctg cgcgacatgg ttgagcagaa gtattttccg attgtgggtc
     6721 gcggctgggc gagattaacc aaagagaaac caggtgagct ggcgatcagc tggatgcata
     6781 ttccacaact gaatggtcag gatcagcaac tgacactcac ggttggggaa aacggccact
     6841 atacgctgga aggtgaagag ttcaccgtca atggtatggt cggccagcgt ctggaaaaag
     6901 atggcgttgc gctgactatc gcggatatta aggccaaacc aggaacacag tttgtcctga
     6961 gccagcgtac cgaactggaa gcgattaacg cgttgcagga aacctttacc gttagcgaac
     7021 gcagtaaaga aagcgggatg ctggaactta ccatgaccgg tgatgatccc cagttgatta
     7081 cccgtattct gaacagcatc gctaacaact atttgcaaca gaatatcgct cgccaggcgg
     7141 cgcaggattc acaaagcctt gaattcttac agcgccagtt gcctgaagtg cgcagcgagc
     7201 tggaccaggc ggaagaaaaa ctcaacgttt atcgccagca gcgcgattcg gttgacctta
     7261 atctggaagc caaagccgtt cttgagcaga ttgtgaacgt tgataatcaa ctcaatgagc
     7321 tgaccttccg cgaggcagag atctcccagc tgtataagaa agatcaccca acttatcgtg
     7381 cgctgctgga aaaacgccag acgctggagc aagaacgcaa acgcctgaat aagcgggtat
     7441 cggcaatgcc ttccacccaa caggaagtgt tgcgtttaag tcgtgacgta gaagcgggcc
     7501 gtgcggtata tctgcaatta cttaaccgcc agcaggagtt gagtatttcg aaatccagtg
     7561 cgattggtaa cgtgcggatt atcgacccgg cagtcactca gccgcagcca gtgaaaccga
     7621 aaaaagcgtt gaatgtggtg cttggtttta ttcttggcct gtttatttcg gtgggtgccg
     7681 tgctggcgcg tgcgatgttg cgtcgtggtg tagaagcccc ggaacaactg gaagagcacg
     7741 gcatcagcgt ttatgccacc atcccgatgt ccgagtggct ggataaacgt acccgtctgc
     7801 gtaagaaaaa tttattttct aatcagcagc gccatcgtac taaaaatatc cccttcctgg
     7861 cggtggataa cccggcggat tctgctgtgg aagccgtacg tgcgctacga accagtctgc
     7921 atttcgctat gatggagact gagaataacg ttctgatgat caccggtgcg acgccagaca
     7981 gtggtaaaac atttgtcagt tcaactctgg cagcggtgat cgcccagtcc gatcaaaaag
     8041 tgttatttat tgatgccgac ttacgccgtg gttattcgca taacctgttt accgtgagta
     8101 atgaacatgg cttgtcggaa tatctggcag gtaaagatga gctcaacaaa gtgatccagc
     8161 attttggcaa aggaggcttt gatgtgatta ctcgcggtca ggtgccacct aacccgtctg
     8221 aactgctgat gcgcgatcgg atgcgtcaat tactggaatg ggcgaacgac cattacgatc
     8281 tggtgattgt tgatacgccg ccgatgctag cggtgagcga tgctgcggtc gtggggcgct
     8341 ctgtcggcac cagcctgctg gttgcgcgtt ttggcttgaa caccgccaaa gaggtgagct
     8401 tgtcaatgca gcgtctggaa caggctggcg ttaatattaa aggcgcaatc ctcaatggtg
     8461 tgattaaacg cgccagcacc gcttacagtt acggctataa ctattacggt tatagttact
     8521 ccgagaaaga gtaaaactga aggtaaagtg accggatgcg gcgtgaacgc cttttccggt
     8581 ctacgcatta gacagttctt cgtttgtcat cagcctcaga gcattcaggt agctgaatgc
     8641 tcttttttat gcattacaaa ctgcacgccg gtatgcgtgc ttcattcacg atttgcgtaa
     8701 aaccggccaa cgaacacatg ccctgcgcat ttcgctcttc acatcctgcc agggtcagtt
     8761 tcacctctcc gggcggcgta tttaatgaca gcggcgtttt atcacgcatc tgctgtaaag
     8821 tctggaagac cagcgaaacc tgaatccact ggctgttatc gcttagccga cgccagcgtt
     8881 caaacaccag ttcaccacct ggcggcgtgt tatccggctg acctggaagc gtccagttga
     8941 gttccagtgc gccgccgaga tttgccagat tagtatcgtg tccggcgata aacagcactg
     9001 aagtgggtaa tgtcacacca tacgcctgtt tttgcggtga atggggcgtc aacgctgtca
     9061 tgatcaaatc caacaacggg gtggcgcggc tgcgggcaac ctctggcgtg cgttgtagta
     9121 aataaaattg cgcgttatgc aaacttagca acgtgttcca ctggtgtgaa tcagtgatcc
     9181 ttccccaccc cggctccggc attccctgtg cctgttgcag gagaaatatt tccgtcagca
     9241 ttgatgcgag gcttaccgca ccggttaatg agacattgtc ggcgctcacc ttgagttccg
     9301 atggtaatgc ctgcgttaat gaacagcttt cgtcctgttt ctcacggtta aggcacaagt
     9361 ttgattgcgg gaaattaagc acccgttcca gttcgcgaaa cgccgtttgc cgatgcccgg
     9421 taaagtcagc aattgaccct cctgccctgc tgaggatcgc gtcagtcacg ttcgcgttat
     9481 ccagttggca aacgccagtt tttagaggat taaataacgg atcgggactg gacgtatcta
     9541 cctgggtatg tacggttatt gcacagtcag gtgccagccc ggcggcgaag gcttcgcctg
     9601 ttttacgggt acgctcgtcg acatcagcaa taatcgcgac ctgaccagac tgcgggcagc
     9661 cctttttcgc cagcaatccg tcggttacta gacgctggcg ttggtaatgt ccgagatagg
     9721 cgattagctc accaccgcgc ggtgtcagcc aacccagttt taccggccag gttggccatg
     9781 cgtctggggt gacatcctgc atcagttgcg tggccttggt tggtgcacgc acaccatgac
     9841 gactgacaat caccacactt tccagcttca gttccggctc actctgagcg aatgtggatt
     9901 gcggggttaa cggaatcaga agagataaaa atgggatcaa gatcgctttc atcgatatgt
     9961 ttccgctttt catatccgac attattgatt gcctgatgcg acgctaatgc gccgcatcag
    10021 acttacgttc gttaactttt caatcgcggg tccagccata ccaacacaag ggtggagagc
    10081 gaacacatca acaaaatgcc gacgaaccaa agtaaatacc acattgttcc tgctccttag
    10141 tacagctcgt tttcgttacg gcggagagtt tctgtcgtca tgcgtcccca cattttgtag
    10201 tagctccaga gagtgtagag caacacaatg ggcaaaaata tcagcacgat taccaacata
    10261 atgctcagcg tcagctgact ggaagtgctg tcccacaacg tcaggctgga gattggactc
    10321 acgcttgacg gcatgacaaa ggggaaaagc gtgatgcctg ccgtgaaaat cacgccaaat
    10381 tgcatcaatg acgccatcaa aaatccccaa cccgggcgac cacgataaat cgccatcacc
    10441 gtcagcaatg ggcagaagaa ccccagcagc gggaagatcc acaaaacggg cgattcgaca
    10501 aaattattca tccacgcacc aggtagcact gccaccagtt tcattaacgg attggaagga
    10561 ccgttagcat cctgggcgag cagtacaaag ccatcaatac cgacccacag ccagtaaccc
    10621 gccagcaaaa agcacagcat caccagtagt gcggcgcgtt tggtcgccag ctgtgaacgc
    10681 agatgaatca caccaacagt tttcagttgt aaccagacgc caccttgcaa aatcaccatc
    10741 ccaaggctga gcaatccgca caataaaggg aatggcgtca gcagttgcca gaagctgccg
    10801 agatactcca cgcgtaattg cggtgtgaag gcaaacggca cgccgagcaa caagttgccg
    10861 aaggcgatac cgaagactac cggcggcacc agactgccga tgaccagacc ggcatcccac
    10921 attttacgcc agcgtgcatc ggcgattttt ccgcgataat caaaggccag cgggcggaag
    10981 aacagcgagc acagcaccag gatcatcgcc acataaaagc cggaaaacgc cgctgcatac
    11041 actctgggcc aggcggcaaa taatgcccca cctgcgagga tcaaccagac ctgattgcct
    11101 tcccagtgcg cgccaacgct attaatcact atccggcgtt catcatcatc acgcgccacc
    11161 agcggcagca aacagccaat ccccatgtca aatccgtcgg agatcataaa gaccaccagg
    11221 atcacgccaa tcagcagcca ccagatgaag cgcaatgttt cataatcaaa catgattttc
    11281 tcctttaccc ctgttgctgc gtcggttgtt cactctgcat cgcgctcggc cccagacggg
    11341 catatttctg catcaggtag acttcggcga ttaagaacag ggtgtaaagc cctacgatca
    11401 tgatcagtga gaaagccagt tgtcctgtgg ttaaagcgga gtgcgcggag tatgtcggta
    11461 agatgtcctg tatcgcccac ggctgacgac caaactcggt cataaaccac ccggcttcaa
    11521 tcgcaatcca cggcaacggc aaactccaga gcgccatttt cagcacccag cgatgctggt
    11581 cgattttgcc acgcagcgtc tggacaagcg caatcagcat caccagtagc agcagggaac
    11641 cacagcccac catgatgcgg aaactccaga ataccggcgc aacctgaggt atcgcgccac
    11701 gcatcgccgc ctggtactgt gcggctgtga catgattcat atccggcgca tagcgggaga
    11761 gcaacatgcc gtagcccagg tcaccttcca gttcccggaa tgcctgcaac acatgcggct
    11821 cacgattgcc ttgcgatatt tcctgcatta acagccaggc catacgtccg cgttgcaagc
    11881 gtgggtaggt ttcagccatc aaattcttca gacccggcac gggtttatct aatgagtgag
    11941 tggcgaggat ccctagcagc gcgggaattt tgatagcaaa ggcgttacgc tcttgatcct
    12001 gttccggcca ggcaaccaca tggaacggtg caggtgcagg ttccgtttgc cactcccctt
    12061 ccatcgccgc cagttttacc ggctgtactt gcgcgacttc gtacgcagaa ctgtctccaa
    12121 gttgcagcgt accgataatc gccagagtac cgaagacgga accgatggca aacgagcgta
    12181 atgcgacatc gcgctcccgt ccgcgcagta aataccaggc gctgatcgcc ataataaaca
    12241 tggccccggt tacgtagccc gccattacgg tgtgcacaaa tttcacctgg ctgaccgggt
    12301 taaagaccag ctcgctgaag ctggtcatct ccatacgcag ggtgtcgata tcaaaatgcg
    12361 caccggtcgg gtattgcatc caaccgttgg cattcaatat ccacaatgca gagagatttg
    12421 aaccgaacgc caccagccac gtcaccagca ggtgctggta tttattcagc cgctgccagc
    12481 cgaagaagaa aagcccgaca aaggtggatt cgaggaagaa ggccattaat gcttccatcg
    12541 ccagcggtgc gccaaaaata tcgcccacat agttggaata gaatgaccag ttagtaccaa
    12601 actgaaactc catggtcagg ccggtagcca cgccaagagc aaagttgata ccgaagagct
    12661 taccccagaa gcgcgtcata tcgcggtaga ttgttttgcc ggtgaccacg taaatggttt
    12721 ccatgatagc cagcaaaaaa atcagcccca gggtaagggg tacaaataaa aagtgataca
    12781 gcgcggtcag agcaaactgc cagcgcgata aatcaatgac atcccacatg cgcactcctg
    12841 taggcgaaga gagaaaaata acccgataat ggtagatctc cctctttatc ctgaaactct
    12901 acgaccagag gtattcgccg attgcgatat tcataagact gaaagcgata cttaccgtct
    12961 ttttacgtcg gtgcagcttc cgccagccac tgacatacct cgctaagccg ctgcgcagag
    13021 tcgaccaaat cttctggcgc tgccagcacc acttccggta ttgggcagat ttcataactt
    13081 tccagtaacg ggccttttaa ggtgtccgta cagcgtaaat gccagacatg gcgtaacccc
    13141 gtggcgttga tatagctctc gccatagcca atggtacgaa tctgaatatt tcccggccca
    13201 cagagacgtg agagaaaaag ccgatcagcc tcgctgatgg gtaattgcgt cagattaatg
    13261 ctgtgcggct gcgcgtcggg gttacgtaca tgcgccagta actcatgcgc caacggtagg
    13321 ccattcatca ggccatcaat gggcggcggt aacagcgaat ctgtcggcaa gagattttgc
    13381 gtggccgcct gccacaacgc cagcggcgcg cagccagcct ccagtttgtc ctccagcaac
    13441 ttttcgccgc gacgtctgcg cacccgccat aatccgcaga agatcgcctc ctgtatttca
    13501 ctttcactgt cgtcagcctg ctgaatgcgc actgacactt ccccttcccc gagaagcgta
    13561 ttgataaagt gacgatcgtc ggcgttgaga tttaacagat cgactgccag cacctcgccc
    13621 aacggcggtt ggcgttcgct tagttgatgt tgtaactcgt ttaacagcgc aatcacctgc
    13681 gggctgtgag cacattgctc cagggccgcc atactcggtt catcattcac ctgacaggtg
    13741 atcggcagtg gattcatgct gaaactgtca tcgttcggtt gcgttcctgg ccccagcaga
    13801 tggaaaaaag tttcgctcat gaggcacgct cctgctgcgg ttcgacaagc ccgcgcatca
    13861 ggtttatcag ttccgcccac gggtgaatac cattcagcac gccgcgatag tttccgccgg
    13921 taaacactaa agtggcagga aagcgaaaga cgccaaagcg atcgccgatg gcttcgctct
    13981 gctcaaggtc agcaatcgcc acctgccatg tatagtcggg aaactcgcgc agtaattcgc
    14041 caatcattac cggattatcg ctgacctctg gcgtgcgttt cgggtcactg cttaataaca
    14101 ccacgccgtc tggcgcttgc gtaagccagt cgtcaagacg ggattcactg actggcgtcc
    14161 agccgcgcgc cagcattcgt tgccacaacg catcaaatgg cgtgtcgttg ctcatccctg
    14221 ctcctcctgt gtcatccggt actggcgcaa gcgaacgcct tcgtaatttt ccatcgacag
    14281 acagtcataa ttgagacagc gcgattcatt agccggttgc ggcacaattc cccacgcagc
    14341 cagctgcgcc agcgccgcct gttccgcagc gggcagttgc tcccgtgcca gttcgctcag
    14401 gctaccgccg tagtcgtcga gcattgcggg ttgcagaccg acgagggcaa tatgtgctgg
    14461 cagatgtccg cggatatccg ccagcgccaa cacttcggag aaactgttct gatgcaggct
    14521 cattttcttc gcgctgagat aagccggaat gcgttctccg gcataggttc gcagcgttcc
    14581 aggttccagc ccgtagtcaa tggcatcgag aatcaacaga tggctggcgc tttcgacata
    14641 ccccagcaag ttcagtccct gagtaccgcc atcgacaatc tccacatact cgggccagtg
    14701 gtaatgggca tacaaccgtt ccgccacccg cacgccgaaa ccttcatcgg cccacagcag
    14761 gttgcccagc cccattacca ccacgcgttg ctcgctcatg aacgctcctt gttacttatt
    14821 ttgccaaatt tgtggctacg gtagccgttg accatggtgg agatcaccgt gtcgtcggac
    14881 atgatgtctt cacgcagcgc catatagaca tgaccgatca caaacgcgcc aatcagccac
    14941 atccccagcc gatgccagct gtgaatgtcc attgagttgc ctcccgtcca gtagaaaaat
    15001 tccaccacat aacggaacgg cgcaaaaata gcgtactggc tgtgttcgct gtacagcgca
    15061 aagccggtga tgatcataaa gaccgacatc aggaaatagc cgaacatcgc cgcctgggcg
    15121 atggggttat ggcctatatc ggcactcgga cgttttgcca gaaacagata ccagcggatt
    15181 tcataccaca cgccctgcca ccagctttta cgccataccg gcacgataaa cagctcgcgg
    15241 gagtatcgat tgccaacaaa agcccagtag atccgcatca gcaagaccac ggtaaaaacc
    15301 atcccggcgc tgaagtgaat taacctgatg tagcccatat agaacagata cgtcgcctcg
    15361 ccgctgacgg aaggtagcgg cttgccgata aagtatccgg tgaccatcaa caccgccatg
    15421 cataacaccg tcagccagtg ccagatgcgg actggcgctt caaagacata gtggctgaca
    15481 acgttgtcgc ttttctgttg catgatgatt ctccttcgct gttaacgcac ctgcacggag
    15541 atcagctcgc taccgtcgtc gcccagcacg tgtgttgaac aggcgaggca cgggtcaaag
    15601 ctgtgcagag tacgcaggat ctccagcggt tgctcgggga tcgccatttt ggtgttcatc
    15661 agcgccgctt cataagcgcc aatctgccct ttgggatcgc gcgggctggc gttccaggtg
    15721 gtcggcacca cgcactggta gagatcaatc ttgccatcgc gaatggcggc ccagtggcct
    15781 aacgccccgc gcggcgcttc ggtaaaaccg acaccacggc actctgtcgg ccaggttgtg
    15841 ggttcccatt tttccgtgga ggcggtggca agattgccgt ttttcaggtt agtcatcagc
    15901 ttgttgaaga aatactgcaa cttacctgcg gcccactgtg cttcgtgcgc gcggcacaaa
    15961 atgcggccca acgttgactg gataccggaa agcggcaggt tcagcgccga catcatgcga
    16021 tcgaccgact caacggtcgc agcatcgcct ttgtgataag cgattaacgt gcgtgccagc
    16081 ggccccactt ccatcgcgtt accgcgccag cggggcgctt tgatccacga gtagcgttcc
    16141 tgttcattca gctgctgaat gttggtatcg ctgcctttga catcgccggg gttgtaccac
    16201 gggtcggtaa tgccatcgaa cggatgacgc ccgacctgat cgttgggata tcgataccag
    16261 gcgtggtcga caaactcttg cacctgctgc ggatcaacca aatccactgg cagcacattg
    16321 ttgaagtcgc cgttaatcac cgcgccgcca ggcatcagca gacttttctc gccaaagtcg
    16381 ttggcaatat ccgggaatgc gccgtagctg aggacacatt tatcagaaag acccgtgccg
    16441 atttcgctcc acggtttgtt gaactgacca atggctaagg cgtcggggat catcacgttg
    16501 ttaatgaaat ccgccgtgcg ggtgataatc gactgcacca ggttcaggcg ttccatattg
    16561 accgccccga ccgcgccgct ttcgtcaatg ttaatggcgc aaggcatccc gccgacaatc
    16621 cagtttggat gcgggttttt accgccaaag accgcgtgga ttttgacaat ttcacgctgg
    16681 aaatcgagag cttcgagata gtgggcaaag cccatcaggt tagcttctgg cggcagtttg
    16741 tactgcgggt gcccccagta gccattgcgg aagatcccca actgcccgcc ttcaacgaat
    16801 ttcttcaggc gattttgtac gtcgaagaaa tagccagggg atgatttcgg ccaagaggag
    16861 agactttgcg ccagttcgga ggttttccgc gggtcggctt tcagcgcatc taacacatcg
    16921 atccagtcca tcccggcaag ctgatagaag tgcaccagat gatcgtggca ccagagcgtt
    16981 gccagaataa tgttgcggat gatattggcg ttgtccggca ctttaatacc gatggcatct
    17041 tcgatggcgt aaaccgaagc cagggcgtgt acgccagtac agacgccgca gatacgttca
    17101 acgaacgccc acgcatcgcg cgggtcgcgc ccttgcagga tgatctccag cccgcgaaac
    17161 atggtgccgc aggagacggc attggtgatc acattctgat cgttaatatt cacttcgcag
    17221 cgcatgtggc cttcaatgcg cgtaatcggg tcgaccacca ggcggcgtcc ggcattattg
    17281 atggtgtatc cctgagtttc gtactgagtg ctcatgcctg tttatcctca ttgcctggct
    17341 gatgttcggt ttctgtaggt tgctggttat gacgtctgcg ctggtcaatg gcgctggcga
    17401 ctgcgtgcac accaacagcc gcagccacca cgccaagcgc ggttaaaccg acggtatcgg
    17461 cggtggaatg agtacccatt tgcggaatat cgaccacgcg gctgtagaac gaaccgcgat
    17521 cccagaaacc attttccgca cagcccaggc agccgtgacc agactggatt gggaaagaaa
    17581 cgccatcatt ccagcgtgtg gaggaacagg cgttataggt ggtaggccct ttgcagccca
    17641 ttttgtacag gcaataacct ttgcgggcag cgtcatcatc ccaactctgg acgaactctc
    17701 cggcgtcgaa gtgagcgcgg cgatagcatt tatcgtggat acgctgacca taaaacatca
    17761 gtggacgacc cattctgtcg acatctggca agcgatcaaa ggtcaccatg taagtaatga
    17821 tggcgctcat cacatccggg atcggcgggc agccaggtac tttgataatg ggtttgtcgg
    17881 tgatgacttt gtcgataggc gttgcctgtg tcggattagg tcgcgcggcc tgcacgcagc
    17941 cccaggacgc gcaggttccc caggcgataa tcgcgctggc tccggcagcg gcacgtttga
    18001 gtttctcaat aaacggtcga ccgctgctga tacagaacat cccctgctcg cccagcggcg
    18061 gattaccttc tactgcgagg atatatttgc cattgtattg cgtgatgatg tcttcaaaga
    18121 cttcttccgc ctgggttccg gcggcagcca tcaaagtatc gtcgtaatcg agggaaatca
    18181 gggaaaggat gacgtccttt gccagcgggt gagcggagcg gataaaagat tcggtacagc
    18241 aggtgcattc cagaccgtgg atccatacca ctggaatgcg gggtttgttc tccagcgccc
    18301 aggcaatctt tggtgccatt cccgcgccta atcccagcga cgtggcagcc agactacaat
    18361 atttgagaaa gctgcgccgg gtaacgccct gacgccgcat ggcctggtaa aatgtttcct
    18421 cgttattcat atcgcacgtc tcttctcctt gcgacaccgg caggacaccg ggcgcttatg
    18481 gtcggcaagc gacgtccctg tagcaaggca aaccatcgct tcacgtgaga tgctgaaaac
    18541 gaaagctcat ccttctgcac tggcgcacgt cgccagaaag tattgttaat aaagcgtagt
    18601 gaaacttttg cacaaaacaa tacaaactgt gtggatttat cttttagcga taaaaatgga
    18661 cctatttttc tttggcaggg cggtggggat gtttagccgg ttgctaaacg agtaaaagag
    18721 aaggaattca tggtgatgaa atgatcaatc tgctgttatt tcacgctatc aatgcacttt
    18781 tggctgtttt tcaggcaaac aaacaaatta ggggtttaca cgccgcatcg ggatgtttat
    18841 agtgcgcgtc attccggaag tgtggccgag cggttgaagg caccggtctt gaaaaccggc
    18901 gacccgaaag ggttccagag ttcgaatctc tgcgct
//
LOCUS       DAZUCE010000084        18929 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZUCE010000084 DAZUCE010000000
VERSION     DAZUCE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.084"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(50..265)
                     /gene="kil"
                     /locus_tag="ACHY03_002468"
     CDS             complement(50..265)
                     /gene="kil"
                     /locus_tag="ACHY03_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560215.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFS3905684.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIEKRKLYIR
                     SLTTKTCITDCMIKVFIGRDGLPVKAE"
     gene            complement(597..953)
                     /locus_tag="ACHY03_002469"
     CDS             complement(597..953)
                     /locus_tag="ACHY03_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905685.1"
                     /translation="MNETELKHIIALLLEDARQVYRLSPNSATQERIRMAEKALKQES
                     ENNISEVSANGENEAIEINSNISNGVIASSHKTILMGARLLEVITSELEKNGIKPTPC
                     YLECVLNVIAYSMFRR"
     gene            complement(985..1698)
                     /locus_tag="ACHY03_002470"
     CDS             complement(985..1698)
                     /locus_tag="ACHY03_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905686.1"
                     /translation="MTTPSVLPQKLWRPLAEIKNFVEKMPDGVRLTEVTKKVKTFAEL
                     SGKERNQLIDFIDKRESIIVFKVRKEGSGNGVTFFRHKKYGYPKREGNVTIIKDLQSK
                     LCTRCGQTKSVDDFYSDASKRDGRAIYCKKCESAMKRSRRECNKLILQQQEPEMNNLK
                     AVSPSPEILRKQAEELLKAAEIAEKKRQEDDVFNKKLAPLKLEILQAAGKMQLKLDEF
                     IDCMDEMNKAVQKLKELTA"
     gene            complement(1753..1824)
                     /locus_tag="ACHY03_002471"
     CDS             complement(1753..1824)
                     /locus_tag="ACHY03_002471"
                     /inference="COORDINATES: protein motif:HMM:NF022510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HFS3905687.1"
                     /translation="MVWAIRTTAMIARELSKQKNKAA"
     gene            complement(2052..2390)
                     /locus_tag="ACHY03_002472"
     CDS             complement(2052..2390)
                     /locus_tag="ACHY03_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3905688.1"
                     /translation="MLMVKKTIYVNPNRGQNRKVSDRSLTSRDRRRIARWEKRIAYAL
                     ENGITPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIKDEKYEDIMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(2359..2697)
                     /locus_tag="ACHY03_002473"
     CDS             complement(2359..2697)
                     /locus_tag="ACHY03_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFS3905689.1"
                     /translation="MVTIIWKESKGTAKSRYKARREELIAERRSSERLTRKIALKISG
                     CVRADKAASLGSLRNKKAEEVERKNRSTYYKDSNPLGNKIHAVQKIKLYSKPPYGLIE
                     YAYGEKDYLC"
     gene            3043..3252
                     /locus_tag="ACHY03_002474"
     CDS             3043..3252
                     /locus_tag="ACHY03_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905690.1"
                     /translation="MLIEGEFGVLHQRVVEILGVSLLEVIATGEAISADAIAGMIRVL
                     HHDELDDLAVKLAIDVLLQDMRLCN"
     gene            complement(3405..4112)
                     /locus_tag="ACHY03_002475"
     CDS             complement(3405..4112)
                     /locus_tag="ACHY03_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS3905691.1"
                     /translation="MKTFAERLNAAMSSAGLSQAQLADMVGVSQPAIQKMSSGKTTGS
                     RKMVELANALRVRPEWLSSGIGSMRPQSAEEPSNARESSLKAIAWDDHQNDNDEFVAL
                     PLLNISLSAGGGSCALEESAEFSLVFRRYYLKKMGVPEKAAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTSIRDGKTYAICQADLLRVKTLIATPTSVIIRSINREEYPDEVMNREEF
                     YKNVKIIGRVFWSSHSW"
     gene            4223..4447
                     /locus_tag="ACHY03_002476"
     CDS             4223..4447
                     /locus_tag="ACHY03_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3905692.1"
                     /translation="MNLVIQRALNIVGSQKRLAADCGVSQPAVHKWLRGGKVSPEKVF
                     AIVNATNGQVKAYEIRPDLPHLFPHPNQAE"
     gene            4563..4859
                     /locus_tag="ACHY03_002477"
     CDS             4563..4859
                     /locus_tag="ACHY03_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFS3905693.1"
                     /translation="MSQTSYSKLSQRDIDRAETDLLINLSVLTQRGLAKMIGCHESKV
                     SRTDWRYIAAILCAFQMASDISPISRAFQHAIRVHANKKRPVGAGRSEQILMNI"
     gene            4874..5092
                     /locus_tag="ACHY03_002478"
     CDS             4874..5092
                     /locus_tag="ACHY03_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905694.1"
                     /translation="MEQYTGIILPHLEEFRQQQHQPQQPEADWVNPEIPGPSVKMCSH
                     TNVQSHQPQQPEADWVNPGDTGTVCGME"
     gene            5113..6189
                     /locus_tag="ACHY03_002479"
     CDS             5113..6189
                     /locus_tag="ACHY03_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3905695.1"
                     /translation="MRNYATISPQFWLGDTGRKLRKSGPECMVVALYMMTSPHSNMLG
                     LYYLPVLYIAHETGLDPEGASKGLQMACEAGFCSYDHDSEVVWVHEMAAWQVGESLKP
                     GDNRCAGVRNEYSALLENPFLSSFYDRYKDDFHLDVRRESCRKIEAPSEPLSSQEQEQ
                     EQERDKTLLVHGEKIATDPLVDSCPVLTERPGPAGMTPEADSGRCVQQVLLVEPEQQH
                     QPQQPEADSAMSGKPIGLTRAMPGPSAGRVDYPDVFERVWREYPHRAGSNPKKSAFNA
                     WRARLREGVSPDVVLDGVRRYARYLEATGKAGTEFVQQASTFFGPNRNFENPWSLPKA
                     GAVSLRCVNHISEPDTEIPPGFRG"
     gene            6196..6936
                     /locus_tag="ACHY03_002480"
     CDS             6196..6936
                     /locus_tag="ACHY03_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS3905696.1"
                     /translation="MKNISTGGILERVRRLAPPHVAAPFRTTDEWREWQLAEGRKRSE
                     EVNRQNHQTRVEKILNRSGIQPLHRKCSFGNYRVQNDGQRHALSLAKSIAAELHTGCT
                     NFVFSGKPGTGKNHLAAAIGNWLMAKGRSVIIVTVSDVMSVLHDGYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVILNQIVDRRTASLRSVGMLTNLNHAAMSTLLGERV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            6962..7732
                     /locus_tag="ACHY03_002481"
     CDS             6962..7732
                     /locus_tag="ACHY03_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS3905697.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESGVTEGQALQPEAPDVVTEEVAPKVSADMMIEFIAQEGAKTCEEI
                     AGKFGVTTRKVASTLAVVTATGRLVRVSIKGKFRYKVPGVENGDLSASSSQDEKSHHE
                     AENSASGGELNRSPLMSEGTAKACTDEFIQDVPSFTEKYAGAVPLPPLRAASRELRRA
                     KKQVQKWERVCAALRELNKHREIVMQITDSSSSIASEK"
     gene            7747..8190
                     /locus_tag="ACHY03_002482"
     CDS             7747..8190
                     /locus_tag="ACHY03_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFS3905698.1"
                     /translation="MAKVFTPEEREKIKEQIVEFVRQSGRETLRALEAKTGASRYYIN
                     TLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKAKKPVVVDPGLIRALPDG
                     EIRRYDRRYNTICRECRNSEAMQRVLAFYRRCAQANNSLHSRVLA"
     gene            8203..8703
                     /locus_tag="ACHY03_002483"
     CDS             8203..8703
                     /locus_tag="ACHY03_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905699.1"
                     /translation="MHLNKEHQSMMGKRQTEEAVRDLEKLFGYRETKLLFLVPVIRQK
                     VEEILEENGIPKEYMTDAMEYSLELVRITPDLTWLDEYQQQFEKGAETLLSAADKTEQ
                     KTEPSVFWNTVEAIARSKIFEFNNINHSGRDTHQSSHLEGVSMLLALLREQGECPQST
                     LNDTEQ"
     gene            9006..9263
                     /locus_tag="ACHY03_002484"
     CDS             9006..9263
                     /locus_tag="ACHY03_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905700.1"
                     /translation="MNNKINIPLEIKEINEIIEVTDRPEFTLMHRYETGTDEQKYIMV
                     AALAVMAIERERREKDVMVIPARNDSPDLRWQEPEWDVAIR"
     gene            complement(9314..9673)
                     /locus_tag="ACHY03_002485"
     CDS             complement(9314..9673)
                     /locus_tag="ACHY03_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HFS3905701.1"
                     /translation="MKTVTIRIESMDAFTADVLSAFKAVAEGMTSKNESIVSFPDWQM
                     MHKVLTPKRMDILMAMTGAGELSIREIAALVGRDVKAVHTDVTALISNGLLEKGENGT
                     SFPYDDIHFDFTLGKAA"
     gene            complement(9675..9962)
                     /locus_tag="ACHY03_002486"
     CDS             complement(9675..9962)
                     /locus_tag="ACHY03_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HFS3905702.1"
                     /translation="MPSRLYKEFIRHLGESEYIRIKIWEVAPAVLGSRHNFKYSMAYV
                     VDGVCVMRYDNERGKGDHKHIGAQEISTHFVSIEQLIADFLHDVESIRRGA"
     gene            10311..10976
                     /locus_tag="ACHY03_002487"
                     /pseudo
     CDS             10311..10976
                     /locus_tag="ACHY03_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211406.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            11233..11877
                     /locus_tag="ACHY03_002488"
     CDS             11233..11877
                     /locus_tag="ACHY03_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HFS3905703.1"
                     /translation="MSTQLVFKSHVLETIEHDGKQWFTAATLASALEYSRGDKITQIY
                     NRNSDEFTPCMSINLKMRFNGINNSLREKDVRIFSLRGAHLVAMFATTPVAKEFRRWV
                     LDILDREAEKSAIPLQPHIQPQFTAEDIILLCYVQLWMEKAQQLSKQLYPVMRELNSD
                     YYGKLFDLAYETRHITNRTRDTLLREAAKLDPANYMVHRARSMLAQLRARQFEF"
     gene            11932..12342
                     /locus_tag="ACHY03_002489"
     CDS             11932..12342
                     /locus_tag="ACHY03_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFS3905704.1"
                     /translation="MNQTIFLRSKQQQQFAINAILATTLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMVADISRQVQWCGRWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTSWAVEQGVRFTDRRYE"
     gene            12339..12530
                     /locus_tag="ACHY03_002490"
     CDS             12339..12530
                     /locus_tag="ACHY03_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFS3905705.1"
                     /translation="MRRQRRSITDIVCENCNYLPTKRSRNKPRPIPNESDVKTFNYTS
                     HLWDVRWLRRRARFNSHSG"
     gene            12554..12844
                     /locus_tag="ACHY03_002491"
     CDS             12554..12844
                     /locus_tag="ACHY03_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFS3905706.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGRGMK
                     PDDLLAAWACSDCHNEIDRRTRILDNNDARLYHLEGVIRTQAILLKEGKIKS"
     gene            12841..13203
                     /locus_tag="ACHY03_002492"
     CDS             12841..13203
                     /locus_tag="ACHY03_002492"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS3905707.1"
                     /translation="MNEYEFVLPWPPTVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LRLDIFTKSRLRITIIAEPPDSRRRDLDNILKGLLDSLIYAGFAEDDEQFDDIRVIRG
                     VKVPGGRVGIKITELENI"
     gene            13203..13397
                     /locus_tag="ACHY03_002493"
     CDS             13203..13397
                     /locus_tag="ACHY03_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFS3905708.1"
                     /translation="MNAKIQTIPELLICTRGNQTEVARILNCNRATVRKYIDDKDAKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            13390..13824
                     /locus_tag="ACHY03_002494"
     CDS             13390..13824
                     /locus_tag="ACHY03_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFS3905709.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            14332..15279
                     /gene="stxA1c"
                     /locus_tag="ACHY03_002495"
     CDS             14332..15279
                     /gene="stxA1c"
                     /locus_tag="ACHY03_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1c subunit A"
                     /protein_id="HFS3905710.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGIPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSVNAILGSVALILNCHHHASRVARIVP
                     NEFPSMCPVDGRVRGITHNKILWDSSTLGAILIRRAISS"
     gene            15290..15559
                     /gene="stxB1c"
                     /locus_tag="ACHY03_002496"
     CDS             15290..15559
                     /gene="stxB1c"
                     /locus_tag="ACHY03_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1c subunit B"
                     /protein_id="HFS3905711.1"
                     /translation="MKKILLIAASLSFFSASVLAAPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            16340..18250
                     /locus_tag="ACHY03_002497"
     CDS             16340..18250
                     /locus_tag="ACHY03_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFS3905712.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVGR
                     PLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFNGTPANHAARFTEVVDQYRADLAD
                     MAGQCVGGSADSVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPDIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAIL
                     SCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLVD
                     DSDNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKY
                     AFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWHT
                     LAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDT
                     VEVKANKAGAAA"
     gene            18390..18572
                     /locus_tag="ACHY03_002498"
     CDS             18390..18572
                     /locus_tag="ACHY03_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFS3905713.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            18598..18870
                     /locus_tag="ACHY03_002499"
     CDS             18598..18870
                     /locus_tag="ACHY03_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFS3905714.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
BASE COUNT         5019 a         4150 c         4704 g         5056 t
ORIGIN      
        1 cggtttgtct gttacagata tggaaggtgt attttattac tcgtcacaac tactctgctt
       61 ttacaggtaa gccatcgcgc ccgatgaaaa ctttaatcat gcagtcggta atgcatgttt
      121 ttgtagtcag gctacgaata tagagctttc tcttttcaat attgtttgct gaagcgatat
      181 atgtgcggcc ttcatgaaga acataatcgc caggtgtcac gcactgacgt ggtatttcat
      241 cagttccgaa gtgatgagca atcataatta tctccatttt cacaaatgaa ctttgttgat
      301 gcggtgccta gtgcctccag gtgacgttaa ccagttaaca attaacgccg gagcagggga
      361 tgatgatttt ccgtgacatc ctgtcggttt aactgttccg cgtgcgcata gccgcattca
      421 ccgcatcata aaattcactt ttaaaaaggg cggacatcag caatcggcaa accgatgtcc
      481 gccaaagagg atattgatac atggatgttg cagcggggtt gtcactcagg cgtctggggc
      541 gatttccatt atttacgatg cacttgaaac attccttgat cgaaaacttt catctgtcac
      601 cgacggaaca tcgagtaagc aataacattt aacacacact ctaaataaca aggggtgggc
      661 ttgataccgt tcttttcaag ctcactcgta atcacctcca gaagtcgtgc gcccatgaga
      721 attgtcttgt gggatgaagc tattacgccg ttgctgatat tgctgtttat ttctattgct
      781 tcattttcac cattcgcgct aacttcggat atgttatttt cactctcctg tttcagagct
      841 ttttctgcca tccggatgcg ttcctgcgtt gcggagtttg ggctaagtcg ataaacctgt
      901 ctggcatctt ccagaagcag ggcgataatg tgcttcagtt ctgtttcgtt catagttaac
      961 tccggtagtt gcaatttatt aatatcaggc ggtcagctct ttaagcttct gaactgcttt
     1021 attcatttca tccatacagt cgatgaattc gtccagttta agctgcattt ttccggcggc
     1081 ctgaagaatt tcaagtttta agggcgcaag ttttttgttg aatacatcat cttcctggcg
     1141 ttttttctcc gcaatttcgg cggctttcag caattcttcc gcctgctttc tgagtatttc
     1201 tggtgaaggt gaaactgctt ttaggttatt catttcaggt tcctgttgtt gcagaattaa
     1261 tttgttgcat tctctgcgtg agcgtttcat cgcagattcg cacttcttgc aataaatggc
     1321 tctcccgtca cgtttgctgg catctgaata aaaatcatcg actgattttg tctgcccgca
     1381 tctggtacat aattttgatt gaaggtcctt aatgattgtg acgtttcctt cccgcttggg
     1441 atatccatat tttttgtggc ggaaaaaggt tactccgtta ccagaacctt cttttctgac
     1501 cttaaataca atgatgcttt cccgtttatc gataaaatct atgagctggt ttctctcctt
     1561 tcctgacagt tcggcaaatg tcttaacttt tttagtaact tccgtaaggc gaacgccgtc
     1621 aggcattttt tcaacgaagt ttttaatctc tgcaagcgga cgccataatt tttgcggcaa
     1681 aactgaaggc gtggtcatca atcacctcgc ttttttttac tgatttgttt atgccaaaaa
     1741 taaaggccac catcaggcag ccttgttttt ctgtttgcta agttctctgg caatcatggc
     1801 cgtagttcgt attgcccaca ccattgattt gtatcaatag tcgtagtcat ccggatagtc
     1861 ctggtattgt tccatcacat cctgtggatg ctcttcgaac tcttcaaatt cttcttccat
     1921 atctcacttc aaataagtgg tttgctgcct aattttattt tctggcgacc aacacaagtc
     1981 acacccgttt cactgcgtgg cttgcggtag taaattttgt tagtccaaac aataaaatct
     2041 accgaagtgg gttatgacca ttttttattt ggatttcgtt ggtgagcgtg gttaacaact
     2101 ctgtgcatta tatcctcata tttttcatct ttaatttttt caacatctcg aggaaatggt
     2161 gttgctaatg ctttgtcaac tttgtccatt gggtcttcat taatcttata ttccggaccg
     2221 tcatctatag cattaaatcc aggtgttata ccgttttcta atgcatatgc tattctcttt
     2281 tcccatctcg ctatcctcct tctgtctcga gatgtaaggc ttctatcaga tacttttctg
     2341 ttttgtccgc gattaggatt aacataaata gtctttttca ccataagcat actcaataag
     2401 tccatacggt ggtttactgt acaattttat tttttgaact gcatgtattt tgtttcctaa
     2461 tgggtttgaa tccttgtaat aagtacttct gtttttgcgc tcgacttctt ctgccttctt
     2521 gttgcgaagg ctgccgagtg atgctgcttt gtctgctctg acgcaaccag agatctttag
     2581 cgcaatcttc cgcgtcagtc tttcactact gcgccgctct gcaataagtt cttccctgcg
     2641 agctttatag cggctttttg ccgtaccttt ggattctttc cagattatgg ttaccatgat
     2701 ggtctccttt aagtggcttt ggtgtatgac gcgtcgaggt gtttttcttc tcgatcgcgg
     2761 ccttgcaact gaaattcgcg tcatccccaa aaccacttag attttggtct caacggttag
     2821 gttgagagtc catcaatgtt aaagagcctg ccaatctgtt ccgtttggct tccagcgtcc
     2881 tgctgttgaa ttgaagataa cctaagttat ctgattgtgc aataactata tttatcattt
     2941 tatggaaagc gttataaatg gctgataaca aaaatatttt attttttgtg gtatccgcgt
     3001 gatattttca agggggaaag gctgattgtt atgggtgatt gcatgttaat cgaaggggaa
     3061 tttggtgtgt tgcaccagcg ggtagtcgaa attctagggg tgtcgttgct tgaggttatt
     3121 gctactgggg aagctatttc agcagatgct attgcgggaa tgatccgagt gcttcaccat
     3181 gatgaattgg atgatctcgc tgtgaagtta gctatagatg tgttacttca ggatatgcga
     3241 ctgtgtaatt aagtaaataa aacccggcac tggagccggg ggatttagaa atggcatttt
     3301 atgaattggc tacttggcga atggtttacc atcggtggta ttgagcacaa taaaaggccg
     3361 ttcttcgtga gtgttcttat agcaaaagtt cagttggagt ctgattacca gctatgtgat
     3421 gaccagaaca ctcgtcctat gatttttacg tttttgtaga attcttctct gttcattact
     3481 tcatcaggat actcttcgcg gtttatcgat ctgataatta ccgatgttgg tgtggctatt
     3541 agcgttttta cccttaacaa atcagcttgg caaattgcat atgttttacc atccctgatg
     3601 ctcgtatctt gcgtgtttac ccccaccaca tcaccatcat ggagtgttgg ctccatactt
     3661 tgcccaacaa ccctgaccaa ctttgccgct ttttctggaa ctcccatttt tttcaggtaa
     3721 tagcgcctaa aaactagaga gaattctgcg gactcctcca atgcacaact tcctccgcca
     3781 gctgaaagtg aaatatttag aagggggagc gcaacaaatt cgtcattatc attttgatga
     3841 tcatcccagg cgatagcttt taaagatgat tcccgagcat tagatggctc ttctgcgctc
     3901 tgtggtctca ttgaccctat accagagctt agccactcag ggcgaactct taacgcgttg
     3961 gctaattcga ccattttacg tgatccggtt gttttaccag atgacatctt ttgtatggct
     4021 ggttgtgata cccccaccat gtctgcaagt tgtgcttgtg acaacccggc tgaactcatg
     4081 gcggcattta gtctttctgc gaatgttttc atacctgtaa tctataacca cggttatcaa
     4141 aagtaaaaca acaattgtta ttgctctgtt gtataactca tgttattttt ggttatgctt
     4201 tatttgctgt agaggtatgc tcatgaattt agtgattcag cgagccttga atattgttgg
     4261 cagtcaaaaa cgacttgcag ctgattgcgg cgtatcacag cccgctgttc ataaatggtt
     4321 gcgaggcgga aaagtttctc ctgaaaaagt tttcgctatc gttaatgcca ccaatggtca
     4381 ggttaaggct tacgaaattc gcccggactt accgcacctg tttcctcatc cgaaccaggc
     4441 tgaataagta acaccgctct ttaacattgc tggtcgttca cctctaacag ggtgagcaaa
     4501 catcagtggc aaacccattg ggattgccgc ttaaccccat atcaatatag gaaaattaac
     4561 aaatgtcaca aacaagttac agcaaactgt cgcagcgcga tatcgatcgc gctgaaacgg
     4621 atttacttat caacctgtca gttctgacgc aaaggggact ggcgaagatg attggctgcc
     4681 atgaatcgaa ggtcagtcgt accgactggc gatacatcgc ggcgatttta tgtgcgttcc
     4741 agatggcatc tgatatcagt ccgatcagcc gggctttcca gcatgccatt cgcgttcatg
     4801 caaataaaaa acgtccggtt ggggccggac gttctgagca aatcctgatg aatatctgat
     4861 attcaggcag ggcatggagc aatacacggg aataattctg ccacatctgg aagaatttcg
     4921 ccagcaacaa caccaaccgc agcagcctga agccgattgg gttaacccgg agataccggg
     4981 accgtctgtg aagatgtgca gtcacaccaa tgtgcagtca caccaaccgc agcagcctga
     5041 agccgattgg gttaacccgg gagataccgg gaccgtctgc ggtatggagt aaatcttgta
     5101 tgcgagggga ctatgcgtaa ttacgcaaca atttcacctc agttctggtt aggcgataca
     5161 gggcgaaaac taaggaagtc tggtccggaa tgtatggtag tggcgttgta tatgatgacc
     5221 tcgcctcatt ccaatatgct gggcctttat tacctgcctg ttttgtacat tgctcacgaa
     5281 accggacttg atcctgaagg ggcttctaag gggcttcaaa tggcttgcga ggctggtttt
     5341 tgcagctatg accatgattc tgaggttgta tgggtgcatg aaatggcagc atggcaggtt
     5401 ggtgaatcgc tgaaacctgg cgataaccgt tgtgctgggg taagaaatga atattccgcg
     5461 ttgctggaaa atcctttttt atcatcattt tatgatagat ataaggatga tttccacctg
     5521 gatgtcagac gtgaatcatg tcggaaaatt gaagcccctt cagagcccct ttcaagccaa
     5581 gaacaggaac aggaacagga aagagataaa acccttctgg tccatggcga aaaaatcgcc
     5641 acggatccac tggtggattc ttgtcccgtt ctgactgaac gtccaggacc agctggcatg
     5701 acaccggaag cagattccgg gcgttgtgtg cagcaggtgc tgctcgtcga accggagcaa
     5761 caacaccaac cgcagcagcc tgaagccgat tccgcgatga gcgggaagcc gattgggtta
     5821 acccgggcga tgccgggacc gtctgcggga cgagttgatt atcctgacgt gttcgaacgg
     5881 gtctggcgtg aatatccgca tcgggcaggg tcaaacccga agaaatccgc gttcaatgcc
     5941 tggagggcca gattacgcga aggggtgtca ccggatgtcg tgctggatgg cgtgaggcgt
     6001 tacgcaagat acctggaggc taccgggaaa gcgggaactg aatttgttca gcaggcatcg
     6061 acgttttttg gcccgaacag gaatttcgaa aatccgtggt cgctgccgaa ggctggcgca
     6121 gtcagcctgc gttgcgtgaa tcacatttct gaaccggaca ccgaaattcc gccgggtttc
     6181 agggggtaat cagccatgaa aaacatttcg acaggaggga ttcttgaacg ggtgcgccgt
     6241 ctggcaccac cgcacgtggc agcaccgttc cggacgaccg acgaatggcg ggaatggcaa
     6301 ctggctgagg gccggaagcg cagcgaggaa gttaaccgcc agaatcacca gacgcgggtt
     6361 gaaaaaatcc tgaatcgttc gggcatccag ccgcttcaca ggaagtgctc attcgggaac
     6421 taccgggtgc agaacgacgg tcagcgccat gctctgagtc tggcaaaatc catcgcggca
     6481 gaactgcata ccggctgcac gaatttcgtg ttcagcggta aacctggcac cgggaaaaat
     6541 caccttgcag cagcgattgg caactggctg atggcgaagg ggagaagcgt gattatcgtc
     6601 accgtgtccg atgtcatgag tgtgttgcat gacggctacg acaacggcaa gtctggtgag
     6661 aaatttttac aggaactttg cggggtagac cttctggtcc ttgatgaaat tggcatgcag
     6721 cgggatacgc gcaacgagca ggtcatactg aaccagatcg tcgaccgcag aacggcatca
     6781 ctgcgcagtg tcgggatgct gacgaacctg aatcacgcag ctatgagcac actcctcggc
     6841 gagcgggtga tggaccgtat gaccatgaac ggtggtcgtt gggtgaattt taactgggag
     6901 agctggcgat caaacgttgg gcgtcagggt atgtgagaat ttttgacgag gtaaattttc
     6961 gatggaaacc gtattgcatg cactgaaagc gatgggaaaa gccaattctg ttgaactggc
     7021 ggcgcggctt gatatcagcc gtgaagaagt tctcaacgaa ctgtgggaac tcaaaaaaaa
     7081 tggcgttgtt gataaaacgg gtcacacctg gtttctggct gtcgaaggtg aatccggggt
     7141 aaccgaaggg caggcactac aacctgaagc gccggatgtg gtaaccgaag aggtcgctcc
     7201 aaaagttagc gctgacatga tgattgagtt tatcgctcag gagggggcta aaacctgtga
     7261 agaaatagcg ggtaagttcg gcgttaccac tcgtaaggtt gcttccacgt tggcggtagt
     7321 aacagcaacg gggcgcctgg tgcgcgtgtc tatcaaggga aaattccgtt ataaagtgcc
     7381 tggcgttgaa aacggcgatt tgagcgcgtc atcatcgcag gatgagaaat cacaccacga
     7441 agcagaaaac agcgcgtcag gcggcgaact gaatcgttct ccgttgatgt cagagggcac
     7501 ggcaaaggcc tgtacagatg agtttatcca ggatgttccg tcgttcaccg aaaaatatgc
     7561 tggtgcagtg ccccttccac ctctacgggc cgccagtcgt gaacttcgtc gcgccaaaaa
     7621 acaggtccag aagtgggagc gtgtctgtgc tgcactacgt gagctgaata aacaccggga
     7681 aattgtcatg cagattaccg attcatccag cagtattgca tcggaaaagt gataactgga
     7741 gagcttatgg caaaagtatt tacacccgaa gagcgggaaa aaattaagga gcagatcgtt
     7801 gaatttgtgc gccagagtgg acgtgagacg ttacgggctc tggaagctaa aactggtgca
     7861 tcaaggtatt acataaacac tctcgccaga gaactggtcg caagtggtga tgtttacaat
     7921 tcaggttacg gattattccc gtctgagcag gcgcgtaagg actggcaaaa tgcccgcaaa
     7981 aaactctcga gagcaaaggc aaagaaaccg gttgtcgttg atcccggtct tatccgggca
     8041 ttacccgatg gggaaatacg gcgctacgac agacgctaca acacaatttg tcgcgagtgc
     8101 cgtaacagtg aagcaatgca gcgtgtgctg gcattctata gacgttgcgc acaggcaaat
     8161 aacagtctgc atagccgtgt attggcttaa caggagctaa acatgcattt gaataaagag
     8221 catcaatcca tgatgggcaa gcgccagaca gaagaggctg ttcgcgatct ggaaaaacta
     8281 tttggctatc gtgaaacaaa actgcttttc ctggttccgg ttattcgcca gaaggttgag
     8341 gaaattcttg aagaaaatgg catcccgaaa gaatacatga cggatgccat ggaatattca
     8401 ctcgaactgg tacgaattac tcctgatctg acctggcttg atgagtatca acaacaattt
     8461 gagaaaggcg cagaaacgtt actttctgct gctgataaaa cagagcagaa aacagaacca
     8521 tcggtattct ggaataccgt tgaggctatt gccaggagca agatatttga gtttaacaac
     8581 atcaatcatt ccgggagaga cactcatcaa tcatcacatc ttgagggggt gtccatgctt
     8641 ctagctctgt tgcgagagca aggcgaatgt cctcagtcga cattgaacga cactgagcaa
     8701 tagtccatcc gtattttccg gcaagataaa gatatattgc gtcaaagcca taaatagtcc
     8761 ttggataccc ctctttaata gccagagagt ccccgaagca ttcaagaaag taatttgagc
     8821 gggctgtttc gtaatgaaaa ttccagcgag cctgattgag ttttttgtcc atttatcctc
     8881 cattgttgct ctgttttgaa aaatggagac cttcacgctg caacgtgagg tcgtgcgccg
     8941 gacacgggta aacatccggt attcaaaatt taatgttaac gaatttttaa tattgaggtt
     9001 atgacgtgaa caacaaaatc aatattcctc tcgaaattaa agaaattaac gaaatcattg
     9061 aggttactga tcgccccgag tttaccctca tgcaccgtta tgaaacggga accgacgagc
     9121 aaaaatacat catggttgca gcattggctg tgatggcgat agaaagggag cggagggaaa
     9181 aggacgtaat ggttattccg gcgagaaatg attcaccgga tttgagatgg caggagccgg
     9241 agtgggatgt ggcaatccgg tgattgatat ttctctgggg taaagcgccg ccagaatgac
     9301 ggcgcggtga attttacgca gctttaccca gcgtaaaatc aaagtggatg tcgtcatacg
     9361 gaaaagatgt tccattttct cctttttcca ggaggccgtt gctaatcagt gcggttacat
     9421 ccgtgtgtac ggcttttacg tcacgcccga ccagagcggc aatctcacgg attgacagtt
     9481 ctccggcacc agtcatcgcc atgagtatgt ccatgcgctt aggggtaagc actttatgca
     9541 tcatttgcca gtctgggaat gaaacgatgc tttcgttctt tgacgtcatt ccttcggcaa
     9601 ccgctttaaa ggcagacaac acatctgccg tgaaagcatc cattgattca atacgtattg
     9661 taactgtttt catatcaggc acctctgcga atggattcta catcatggag gaagtcagca
     9721 atgagttgct cgatggaaac aaagtgggta ctgatttcct gagcaccaat gtgtttgtgg
     9781 tcgcctttac ctctttcgtt gtcgtagcgc atgacgcaaa caccatcaac aacgtaagcc
     9841 atgctgtatt tgaagttatg gcggctacca agaacggctg gagccacctc ccagatttta
     9901 atccttatgt attcggattc cccaagatgg cgaatgaact ctttatacag tctggatggc
     9961 ataaaaactc cgtgtattta tgttgttaag gataacaaca gtgcatagcg ttgtcaatta
    10021 atacaacatc aatgctgagt tgacgacaac acttatccgg gattatattc ttcgcacggt
    10081 cgagttggcc gtcgggattg gaaccccgga tagagaccgc gacaaacaca cgccgcgagc
    10141 gtgtttttta ttgtcgtatg cacgcgcaca tctgaattat ggtggggcgc atgggggagc
    10201 tgaaaagctc gccggtcggt ttcccggtag ttccaaccct gtgcgtctca ccacccgatg
    10261 attggaacct tacggtggtg atagtttaga aaccactaga gggcgtcatt atgacaactc
    10321 aaatctctgt tgaaaccatc tccccgatta cccataacca gattcctgtt attaccaccg
    10381 agcttttggc acaactttat ggcaccgaaa ctaataacat caaagtgaac tatacacgca
    10441 atgccgagcg ttttgtttgt gggaaacact acttcaaatt ggaaggggct ggattgcggg
    10501 aatttaagaa caaggttact caaagtaact tagttgcacc gcgtacaaag cacctcatcc
    10561 tctggacaga acgcggggca gcccgtcacg caaaaatgct cgaaactgat caggcatggt
    10621 aagtgttcga aaaactggaa gactgctatt tcagccaggg aaaaacaaca caaaccgaac
    10681 agcagccgca gattcagcca caattcacag ccgaggaaat catcctcctt tgctacatgc
    10741 agctctggat ggaaaaagcc caggacatca gcaaacacct gtaccctata atgaaagagc
    10801 tgaactcctc atacacgaac aagctgtatg acattgcgtt tgagaccatc tacatggtga
    10861 cgaagaacag agacgcgcta ctgagggagg taacacgtct cgacatgtca agttccgtta
    10921 tccagcgggc catgccaatg ctgaaaagcc tgcgggcaag acaattcgag ttttaaggcc
    10981 agatttgaca aatccgcatt aacggggata tattccgcac caaggtgctg aacacaccta
    11041 tacaagcgga agccgcaccc gtcagtcatg cggatttttt atgtccattt ttcagatatg
    11101 gtcgggtagc gcgtataccg aaaaacagcc gcaaggctaa ggatacgggc cgttcttgta
    11161 tgcggtgttc aagtacccga ccgccctgct gaacacaggg attctgaaca aaaaatacaa
    11221 ggacataaaa ctatgagcac tcaactcgta ttcaaatccc acgttcttga aacgatcgaa
    11281 cacgacggca aacagtggtt cactgccgcg actttggctt cagcactgga gtattcgcgc
    11341 ggcgataaaa tcacccagat atacaaccgc aattcggatg agtttactcc ttgtatgtca
    11401 atcaacctca aaatgaggtt caacgggatc aacaatagct tacgagagaa agatgtgcgt
    11461 attttctctc tacgcggtgc ccacctggta gcgatgttcg caacaactcc agtagccaaa
    11521 gagttccgtc gctgggtatt ggatattctg gaccgtgaag ctgaaaaaag cgctataccg
    11581 ctacaacctc atattcagcc gcaattcaca gcagaagaca tcatcctact ctgctacgtt
    11641 caactctgga tggaaaaagc acaacaactc agtaagcagc tataccccgt catgagagaa
    11701 ctgaactctg attactacgg gaaactgttt gatctggcct acgagactcg tcatataacc
    11761 aaccggactc gcgacacatt gctccgcgaa gcagcaaaac ttgatcccgc caactatatg
    11821 gtgcataggg ccagaagcat gctggcgcaa ctacgggcaa gacaatttga attctgaaac
    11881 taaaggagct tcggctcctt ttttgttggg aaaatccaat gagagggaat aatgaaccag
    11941 actatcttcc tccgaagtaa gcagcagcaa caattcgcca ttaacgccat ccttgcaaca
    12001 actctcgata aagacaaacc cgttacgatc cgcatcaccg attacaagcg gaatctcgat
    12061 caaaatgcca aatttcacgc gatggtcgct gatatctccc gacaggttca gtggtgcggc
    12121 agatggctaa aaccagaaca gtggaaagtt ttgttaatca gcggtcatgc cgtggcgaca
    12181 aaacaggaag ctgatgtttt gccaggtctt gaaggtgaat acgtcaatat ccgcgaaagc
    12241 agtgcgcaga tgagcgtgaa gcgtatggca agcctgattg agtacacaac ttcctgggct
    12301 gtggagcagg gtgtcagatt taccgacagg aggtatgaat gagacgacag cgacgaagca
    12361 ttaccgatat agtctgtgaa aactgcaatt accttccaac gaaacgctcc cgaaataaac
    12421 ccaggccaat ccccaacgaa tctgatgtaa aaaccttcaa ttatacgtct cacctgtggg
    12481 atgtccggtg gctcagacgt cgtgcaagat ttaatagtca ctccggatag ttcaatgtac
    12541 gaggaataag atgatggcaa acctacgcaa agaagcgcgt ggcagagaat gtcaggtacg
    12601 gatttacggc gtatgcaatg gcaatcctga aactacagtt ttggcgcatt accgaatggc
    12661 tggaatttgc ggaaggggaa tgaagccaga tgacctgttg gctgcatggg cctgtagtga
    12721 ctgccataat gaaatcgatc gccgtactcg cattctcgac aacaacgacg ccagacttta
    12781 ccacctggaa ggcgtgatca ggacgcaggc gatactgctg aaggagggga aaattaagtc
    12841 atgaatgaat atgagtttgt gcttccctgg ccgccgacgg tgaataccta ctggcgaaga
    12901 cggggaagcc agtactacat cagcgataaa ggccagaaat accgaaaaga cgtacagcaa
    12961 atcatccggc aactcagatt agacattttc actaaatcac gacttcgcat cacaattatt
    13021 gctgaaccac cagattcccg tcgtcgcgac ctcgataaca tcctgaaagg tttactcgac
    13081 tctcttatct acgccggatt tgcggaagac gacgagcaat tcgatgacat tcgcgtaatt
    13141 cgcggcgtga aagtgcctgg cggtagagtg gggataaaaa tcaccgaact ggagaacatt
    13201 tgatgaatgc taaaattcaa acgatacctg aattactgat ctgcaccagg ggaaatcaga
    13261 cagaagtcgc cagaatactg aactgcaatc gtgccacagt cagaaaatac attgatgata
    13321 aagatgcgaa aaagcacgcc gtcgtcaatg gcgtccttat ggttcatcgc ggatggggta
    13381 aagatactga tgcgtgatat ccggcaggtt cttgagcgct ggggtgcatg ggcggcaaat
    13441 aactatgagg atgttacatg gtcgcccatt gctgccggat ttaagggact gatccccgaa
    13501 aaagtaaaat cacgtccaca gtgttgtgac gatgacgcga tgattatatg cgggtgtatg
    13561 gctcgcctta acaggaacaa cagtgatctg catgacttgc tggttgatta ttacgtgttg
    13621 ggggagacgt tcatggcgct ggcacggaaa catgggtgct ctgacacctg tataggtaaa
    13681 cgccttcaca aagcggaggg gattgttgaa ggcatgctga tgatgctggg agtgaggctt
    13741 gagatggatc ggtatgttga gcgtgaattg ccgggaggga gaacctctgt attttatcag
    13801 cgaaaaaata gtttacgatc gtaaaaatct gcatatcatg ataagagtgg ttacattgct
    13861 acgctgctta acccgccgat gcgcgggttt ttttgtaccc agaatcctgt gagctatacg
    13921 gaaagtacac agaaaggaag gtgcgaccgt aattaataac aaaatcttaa aaatcgcata
    13981 tagcactatt agttttctaa atattgtata ttttaagtat tgcaggatga ccctgtaacg
    14041 aagtttgcgt aacagcattt tgctctacga gtttgccagc ctcccccagt ggctggcttt
    14101 ttttatgtcc gtaacatcct gtgtatcaat aaatgttgtt gtctacgtac gtcaagtagt
    14161 cgcatgagat ctgaccagat atgttaaggt tgcagctctc tttgaatatg attatcattt
    14221 tcattacgtt attgttacgt ttatccggtg cgccgtaaaa cgccgtcctt cagggcgtgg
    14281 aggatgtcaa gaatatagtt atcgtatggc gctcaaggag tattgtgtaa tatgaaaata
    14341 ataattttta gagtgctaac ttttttcttt gttatctttt ctgttaatgt ggttgcgaag
    14401 gaatttacct tagatttctc gacagcaaag acgtatgtag attcgctgaa tgtcattcgc
    14461 tctgcaatag gtattccatt acagactatt tcatcaggag gtacgtcttt actgatgatt
    14521 gatagtggca caggggataa tttgtttgca gttgatgtca gagggataga tccagaggaa
    14581 gggcggttta ataatctacg gcttattgtt gaacgaaata atttatatgt gacaggattt
    14641 gttaacagga caaataatgt tttttatcgc tttgctgatt tttcacatgt tacctttcct
    14701 ggtacaactg cggttacatt gtctggtgac agtagctata ccacgttaca gcgtgttgcg
    14761 gggatcagtc gtacggggat gcagataaat cgccattcgt tgactacttc ttatctggat
    14821 ttaatgtcgc atagcggaac ctcactgacg cagtctgtgg caagagcgat gttacggttt
    14881 gttactgtga cagctgaagc tttacgtttt cggcaaattc agaggggatt tcgtacaaca
    14941 cttgatgatc tcagtgggcg ttcttatgta atgactgctg aagatgttga tcttacgttg
    15001 aactggggaa ggttgagtag tgtcctgcct gactatcatg gacaagactc tgttcgtgtt
    15061 ggaagaattt cttttggaag tgttaatgca attctgggta gcgtggcatt aatactgaat
    15121 tgtcatcatc atgcatcgcg agttgccaga attgtaccta atgagtttcc ttctatgtgc
    15181 ccggtagatg gaagagtgcg tgggattacg cacaataaaa tattgtggga ctcatccact
    15241 ctgggggcaa ttttgatacg cagggctatt agcagttgag gggggtaaaa tgaaaaaaat
    15301 attattaata gctgcatcac tttcattttt ttcagcaagt gtgctggctg cgccagattg
    15361 tgtaactggg aaggtggagt atacaaaata taatgatgac gataccttta cagttaaagt
    15421 gggagataaa gaattattta ctaacagatg gaatcttcag tctcttcttc tcagtgcaca
    15481 aattacgggg atgacggtaa ccattaaaac taatgcctgt cataatggag ggggattcag
    15541 cgaggttatt ttccgttgac tcagaatagc ccggtgaaga gaacaggcgg agatttatca
    15601 atgttaaata catctcaatt cagtcagttg attgttgcct gtctgataat agatgtgcta
    15661 caaaatttct gcatggtgaa tccccctgag cggcggggca tatcagcgtc acaggtgttt
    15721 ctgttttacc tctatccttt ctgtgcgggt tcaggtgctg atactgaact caccgggagg
    15781 cacccggcac catgcaatga acggtacata gcgctactct ccagcccctc tccggagggg
    15841 ctttttaatg aggggaagac actgggcagt gaaatgttaa atcactcaca attcaggtag
    15901 ttgacggttg tctgcttggc tgcgaatttg ttaaaaaaat cctgcatggt gaatccccct
    15961 gtgcggaggg gcgactggtg aatggtatga tctctttgat gatcgtaagc gagaatacgc
    16021 gggtttggtg gcaccaggcc gaactcaccg ggaggcaccc ggcaccatgc agtatacaga
    16081 gattaggcat atatcagggc ccctcatagc aggggccttt ttacatgcaa aaaaaagccg
    16141 ccccgggaag agcggttggc aagaagaaaa gcaatatgaa caattaatta acgctgcgaa
    16201 taatacctta cagtaatcac cctgcgcaac tgtgaggagt gtatttcttt tttgcgggtg
    16261 gttgtctttt tcccttgtgt ttcgggactt ccgctcactc cttatcttat tcagtacatt
    16321 atcccggccg ggaggattca tggcatttaa acactatgac gtggtcaggg cggcgtcgcc
    16381 gtcagacctt gcggaacgaa taactcaaaa actgaaggaa gggtggcagc cttatggtag
    16441 tgcgctgatt tcgacagctg gttatggtgc ggagttcatc cagccagttg tgagtgaggg
    16501 gagcatctca tcaccagagg agccaggcaa ccgtccgacg acctcagcgc cttctgttgc
    16561 gccagaatat tactatgtga tcgcgcttgc tggtcagtcc aatggtatgt catacggtga
    16621 gggactgcca ttaccggata cattcgacag ccctgatcca cgtattaaac agttagcgcg
    16681 tcgcagtacg gtgacaccgg gaggtgctgc atgtatctat aacgatatta tcccggcaga
    16741 tcactgtctc catgatgtgg aggatatgac agggcttaat catcccaaag cggatttatc
    16801 gaaaggtcag tacggctgtg tgggacaggg gctgcatatc gccaaaaagc ttctgccatt
    16861 tataccggcg aatgcgggca ttcttcttgt tccgtgctgc cgtggtggtt cagctttcac
    16921 caccggaacc gatggcacat acagtgacac tactggcgcc tcagagagtt ccacccgctg
    16981 gggggtgggc aggccgctgt ataaggacct cattggtcgt acaaaagccg cgctggcgaa
    17041 gaatccgaaa aatgtgctgc ttgccgtggt atggatgcaa ggggaatttg attttaacgg
    17101 aacgccagcg aatcatgcag cccgttttac agaagtggtg gatcaatacc gtgcggacct
    17161 tgcagatatg gcggggcagt gtgtgggtgg ctcagctgac agtgttccct ggatttgtgg
    17221 agacacaaca tatttctgga agcagaagaa cgaaacggcg taccagacgg tgtacggtag
    17281 ttacaaaaat aaaacggaaa agaatatcca tttcgtgccg ttcatgaccg atgagaacgg
    17341 ggtgaatgtg ccgacgaaca aaccggaaga agacccggat attccggata tcgggtatta
    17401 cggttcaaaa tggcgaacgg acagaagcac ctggacatct caggacaggg ccagtcattt
    17461 cagttcatgg gctcgccgtg ggattatttc cgaccgtctg gcaacggcga ttctgagctg
    17521 cgcgggtaag tcttctgcgt ttgttaatgg tactgccggg gtggttgttc cagacagacc
    17581 ggttaccacc tcagagtctg taatttttta cgatgccaaa aaagcttcag acaatcagct
    17641 gaaaccttat ggctgggacg gtatggatgg cagacgcaca ctggttgatg atagcgacaa
    17701 taaagctctg cgaattgaga aaaataacag cgcgaaatcc tggtcaatgt actgtgatat
    17761 tgctgcagac aaggcaaaac ttttactgga aaaaggcggg gaaattgctg tccggtttaa
    17821 aatccccgaa aacgtcaatc ttgagacaac cagaaacaag tatgcctttg gtttgtactg
    17881 gcgaatagcg gaatggccgg gtgagggtgg tgaaggctat ctgagttctt tctttgtcca
    17941 gacagataaa gccagtattg atgttgcata ccatcataca gttaatcaac aaaaagaact
    18001 tggcacgttt ggcgcattcg accatgactg gcatacgctt gcatttaaat ttaagggcag
    18061 taacagcatt aatgttactc cggtgcttga tggtgtggat ggacaggcgt ttgacctggt
    18121 gaaatgggcc aatactgcta atggactcaa caggtttgtc attacggata ttacaggtag
    18181 tgcagaaacc taccctgtac ttattgatac ggtggaagtt aaagcaaaca aagctggagc
    18241 agccgcataa ttgctaaaaa aagccgccag cggcaggaat ggaagctggc ggaggtaatc
    18301 ccaatggaga atgtaaagaa aagatgcttt cgtacattgg ttttttaaat gaaaacagtt
    18361 ctcattgtca accataacgg taagaaatta tgatatttat tcatcaggtg atgctgtact
    18421 tctgtacggc ggtctgtgtg ctgtatcttc tttcgggtgg gtacagggct gtgcgcgatt
    18481 tctggtgcag gcagattgat aaaagggccg ctgagaaaat cagcgccagt cagtcagccg
    18541 gaagcaaacc cgaagagccc gttactcctt aacaacccct ttcagcgaga aaatcccatg
    18601 tcagaaatca catccctggt cactgcagag gcagtgaagg aagtcctgcg ctctgaagaa
    18661 gtccggagcg cactgaaaca gaaacttcgc cagaatcttg aggcgcgtct tgatgcagaa
    18721 gttgatgcca ttctggatga gctgcttggt gtacaggcag agccaccgac tgaagcggga
    18781 gataccaccg cagagagcgg tgaagttcag cctgaatcac cggtcgccga tgcgactgaa
    18841 cctcaacccg aatcggtcat gatgctgtaa cgaggagtca gggccatcag taaacggctg
    18901 ctggcctttt tcatgttgtg agcttccgg
//
LOCUS       DAZUCE010000085        18865 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZUCE010000085 DAZUCE010000000
VERSION     DAZUCE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.085"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(119..484)
                     /gene="yhaH"
                     /locus_tag="ACHY03_002500"
     CDS             complement(119..484)
                     /gene="yhaH"
                     /locus_tag="ACHY03_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS3905715.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(767..1753)
                     /gene="yqjG"
                     /locus_tag="ACHY03_002501"
     CDS             complement(767..1753)
                     /gene="yqjG"
                     /locus_tag="ACHY03_002501"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFS3905716.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRYWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1823..2215)
                     /gene="yqjF"
                     /locus_tag="ACHY03_002502"
     CDS             complement(1823..2215)
                     /gene="yqjF"
                     /locus_tag="ACHY03_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFS3905717.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2401..2700)
                     /gene="yqjK"
                     /locus_tag="ACHY03_002503"
     CDS             complement(2401..2700)
                     /gene="yqjK"
                     /locus_tag="ACHY03_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFS3905718.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2690..3094)
                     /gene="yqjE"
                     /locus_tag="ACHY03_002504"
     CDS             complement(2690..3094)
                     /gene="yqjE"
                     /locus_tag="ACHY03_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS3905719.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3097..3402)
                     /gene="yqjD"
                     /locus_tag="ACHY03_002505"
     CDS             complement(3097..3402)
                     /gene="yqjD"
                     /locus_tag="ACHY03_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFS3905720.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3440..3808)
                     /gene="yqjC"
                     /locus_tag="ACHY03_002506"
     CDS             complement(3440..3808)
                     /gene="yqjC"
                     /locus_tag="ACHY03_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFS3905721.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3955..4338)
                     /gene="mzrA"
                     /locus_tag="ACHY03_002507"
     CDS             complement(3955..4338)
                     /gene="mzrA"
                     /locus_tag="ACHY03_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFS3905722.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPQGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4342..5004)
                     /gene="yqjA"
                     /locus_tag="ACHY03_002508"
     CDS             complement(4342..5004)
                     /gene="yqjA"
                     /locus_tag="ACHY03_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFS3905723.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5026..5109)
                     /gene="yqiM"
                     /locus_tag="ACHY03_002509"
     CDS             complement(5026..5109)
                     /gene="yqiM"
                     /locus_tag="ACHY03_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFS3905724.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(5349..6125)
                     /gene="exuR"
                     /locus_tag="ACHY03_002510"
     CDS             complement(5349..6125)
                     /gene="exuR"
                     /locus_tag="ACHY03_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFS3905725.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6255..7553)
                     /gene="exuT"
                     /locus_tag="ACHY03_002511"
     CDS             complement(6255..7553)
                     /gene="exuT"
                     /locus_tag="ACHY03_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFS3905726.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPTPQH"
     gene            8036..9448
                     /gene="uxaC"
                     /locus_tag="ACHY03_002512"
     CDS             8036..9448
                     /gene="uxaC"
                     /locus_tag="ACHY03_002512"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFS3905727.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9463..10950
                     /gene="uxaA"
                     /locus_tag="ACHY03_002513"
     CDS             9463..10950
                     /gene="uxaA"
                     /locus_tag="ACHY03_002513"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFS3905728.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            11033..11584
                     /gene="ygjV"
                     /locus_tag="ACHY03_002514"
     CDS             11033..11584
                     /gene="ygjV"
                     /locus_tag="ACHY03_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFS3905729.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11589..12833)
                     /gene="sstT"
                     /locus_tag="ACHY03_002515"
     CDS             complement(11589..12833)
                     /gene="sstT"
                     /locus_tag="ACHY03_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFS3905730.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13378..14343)
                     /gene="alx"
                     /locus_tag="ACHY03_002516"
     CDS             complement(13378..14343)
                     /gene="alx"
                     /locus_tag="ACHY03_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFS3905731.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(14627..15613)
                     /gene="ygjR"
                     /locus_tag="ACHY03_002517"
     CDS             complement(14627..15613)
                     /gene="ygjR"
                     /locus_tag="ACHY03_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS3905732.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQAQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(15692..16384)
                     /gene="ygjQ"
                     /locus_tag="ACHY03_002518"
     CDS             complement(15692..16384)
                     /gene="ygjQ"
                     /locus_tag="ACHY03_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFS3905733.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(16461..16964)
                     /gene="ygjP"
                     /locus_tag="ACHY03_002519"
     CDS             complement(16461..16964)
                     /gene="ygjP"
                     /locus_tag="ACHY03_002519"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFS3905734.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17049..18185
                     /gene="rlmG"
                     /locus_tag="ACHY03_002520"
     CDS             17049..18185
                     /gene="rlmG"
                     /locus_tag="ACHY03_002520"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFS3905735.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFIQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            18354..18458
                     /locus_tag="ACHY03_002521"
                     /pseudo
     CDS             18354..18458
                     /locus_tag="ACHY03_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
     gene            18455..>18865
                     /gene="higA"
                     /locus_tag="ACHY03_002522"
     CDS             18455..>18865
                     /gene="higA"
                     /locus_tag="ACHY03_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFS3905736.1"
                     /translation="MITIADILQAGEKLTAVAPFLAGIQNEEQYIQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAVPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFI"
BASE COUNT         4795 a         4897 c         4792 g         4381 t
ORIGIN      
        1 tttataggta atttatataa cgacattgaa atattttaaa taaacattag taaattattc
       61 attcatcaac atgatgataa aagccggatg agtcagacat ccggcgagca gatttgtttt
      121 aaggttccag ttttggatca ggtccaaagc ggttttcgcc tggcgtaccc gcctggcagt
      181 taaagacgat gataataagc cagccgataa acgggattaa gaatagcagt gcccaccacg
      241 ccgagcggtc ggtgtcgtgc aggcggcgga actgaactgc ccaccacggt aaaaacacca
      301 gaataccgta gattgtcgtc aggatgcctt cgccaccagc gcgttgccag cctaacattt
      361 tatccagcag ccccagcacg aacgtaaaga tgatgttgac cagaataaac atccagtact
      421 ctttgcgccg cgcacgaccc cggaaaccga cataattttt tagtactttc agataccagt
      481 ccatttttgc ctcaattttg ctcaatcact tgtttttaaa gcagcaaatt aagaatagac
      541 gtgaatgaaa tggcagtaaa tattatcgcg atgaattaat aaggagctgt gaaggataag
      601 ggcaagcgca aaatcgccag atgcgctacg cttatctggc ctgcgcggtc attgcaacat
      661 attgaatata aatgttttgt aggtttgata aggcgttcac gccgcatccg acatgaacaa
      721 catgcactta gccaattatc agaggcacct cgtaaggtgc ctttttttaa ccgaagcgaa
      781 catctcgtcc atgcggttca tcgagatcct gccacggacc aattgaaata atccccgtag
      841 ggttgatggt cttatggctg cggaagtaat gattacggat gtgatcgaaa ttgactgttt
      901 cggcaattcc cggcatctgg tagatatcac gcaggaagcc atacagatta aggtaatcgc
      961 tgatgcggtg cttatcacac ttgaagtggg tcacatacac tggatcaaaa cggaccagcg
     1021 tggtccacag gcgaatatcg gcttcggtta gctggttgcc ggtcaggtaa cgatgctgac
     1081 ctaaaatctg ttccagtcgc gccagcgatt caaacacttt ctccaccgct tcgtcgtagg
     1141 cttgctggct ggtggcaaaa ccagctttat acacgccgtt gttaacggtg tcataaatcc
     1201 agccgttaag ttcgtcaatt ttcgtttgca gggctggtgg gtagtaatca cccgctttcg
     1261 cgcccagcgc atcaaacgcg gtattaaaca tgcggatgat ttctgctgat tcgttgctga
     1321 cgatggtgtg gttctttttg tcccacagca cgggaacagt aactcgtccg ctgtagtgtg
     1381 gatcggcgtg gagataaagc tgatacaaaa attcatgttg atagagcgtg tctccggttg
     1441 ctcccggaaa actgtcatca aaggtccagc cgttttccag catcagcggg ttcactacgg
     1501 aaacggaaat aaacggttcc agtcctttga gtttgcgcat gatcagcgtg cggtgcgccc
     1561 acgggcaggc gagtgaaaca tagagatgat aacgatcttt ctctgcgata aaaccgcctg
     1621 tgccagtggg gcctggagcg ccatcggcag tgagccagta acgaaatgcg gaagctgaac
     1681 gttgaaattt accgccggta gatttggtat cgtaccaggt gtcatgccag acgccgtcaa
     1741 tcagttgacc catttttctc tcctccaata gcaaaaacga ggagcttcct cctcgttttt
     1801 tatcgttaag tatagcgcgt gcttaccact ttttattcag caggcggtcg atgctatacg
     1861 cgcccggacc ggtaattgcc agcagcagga atccgccaga aattgtcagg tttttcatga
     1921 acatcagcga gttgacgcct tcagcaaagt tgctgtgaaa taaaaatgcc gtcagcagcg
     1981 taaagcccgc agtaaacagg gctgtggtgc gagtcaggaa accgaacagg attgccagac
     2041 caccaccaaa ctcaagcaga atcaccagcg gcagcataaa acccgggacg cccatcgctt
     2101 ccatatattg ttgggtaccc gcgtaaccag taatttttcc ccagcctgcg gtaataaaca
     2161 gaatcggcat taaaatgcgc gctaccagta caccaacatc ttctaatttt ttcatcatac
     2221 tctccactgc gccactcagg ctgctgattg tttttttgtg caagggcgcg gtattagcgt
     2281 cgttgctgtc gatggagaga atcataaacg tggtgaatga tgattgttag caaggaaaac
     2341 tgtcaaaaat cttcaaaaaa tttgatgggt aaggccggaa tggctccggc cagagggaag
     2401 ttaaccgcga agctgttgct gcttgagggt cgttttaacc agacgccagg cgctccatac
     2461 gccaaaaccg cgtctggccc agcggaccag catattagga tggcgaatcg tccagatcgc
     2521 catcacgcta ctgccaacca gcgcccagga gcgcagactt agcagcatat tccagcgacg
     2581 atcgtaagcg cctgttgcct ccagccattc acgacgactg gcagaaagat ccagccgttg
     2641 ttgctggatc tggctaagca gttgcgcctt acgtcgttca cgttcgactt tactgctcac
     2701 gggactcctc ctcgagcagc tgccgatcgt ttgccagctc atggcgtgta tggcgcagca
     2761 aggtggattt acgcgattta cgtaacgtcc agataccgcc aatcagtgcc agtagcagca
     2821 gcaccacggt ggtggcaatc atcgcattca ggcgatattg cggatcaacc gcccaaataa
     2881 ttagcaccat caggctcata agaccaaatg cagcgaaaag catcgtcagg cccagcatca
     2941 gtaaaagttg aaagagatta gctttttcct cttccagctc aaccaccgcc agccgcagac
     3001 gtgtttccac catttcaacc atgatggaaa caattcgctg cccgatgccc agaacgcttt
     3061 taccgggccc ttgcgcgtga tgagtgtccg ccataattaa cgacgcgaca gcagaacgcc
     3121 gagcactaca ccgattgcag cgccaatgcc cacgcccgtc catggatttt cacgcacata
     3181 ctcatcggca cgcgccgccg cgacacgggt ttgtttggcg atggcatcac cggtttcacc
     3241 caggcgataa cggctctgtt tcagtgcctg ctccgctttg ctacgaatct tactcaactc
     3301 ttctttcgac ttctcgccag atgagctaag cacctcttcc agcgtatcgg aaagggattt
     3361 caactcagca cgcagatgtt ccgtagtgtg ttctttcgac atagttttct ccaggtgagt
     3421 aagtagtgac tattgtgagt tagtagtcgc gcgcttccag ctttttcagc tcttcctgcg
     3481 cttctgccag tttccgttcg cgtttggcaa tcttatcggc atcgcctttt tgcttcgcct
     3541 cggctaaatc ttgctggcgt tccgccacct catctttctg ctttgcgatt ttcttctgat
     3601 gatcggcacg cagctggcta tctgaacagt tggcccggac ttcactcagg gctttattca
     3661 gaccgtcaat acgattctgg ttttggtgtt tttcggcata gctgatctcc ttaaggatat
     3721 tttgctcctt ttcctgacac agggtagtgg cataactacc ggcactaaga gcaaaaagag
     3781 aaacagctaa agcgatgcgg tatttcattc ttgaaccttc cattgtgatt cggacctatt
     3841 acaaaggaac gattgtccag acaacgaagc ggtagtgtta accaccgcct cattaatcat
     3901 agacagttac ggtgaaatca ccaaagtgag tgatctgttt cggataacag gaagttatcc
     3961 gaagcgatgt gagttatccc gtagccgggt cagccactgc atagcctgac tgttattgtc
     4021 ctgctgcgca atgatgtagc cttggggcaa tgttctgtcg aggaccgctt ttgcagcggc
     4081 gctctggtcg ctggagtcaa aggtaattaa cagagtgtcg tttttggggg tgatactttt
     4141 gaaaggaatg ccatgagcgt caaggtgatg ccagattgaa aaaccgtctg gcatcgttgt
     4201 gccttgatga acggcacgaa tcgccagcgt agactcttgc tggcgaaccg cggaccaggc
     4261 cagcaacagc gtgccgacca gaagtaaaac agcgccggac caggctagct ggcgaagcga
     4321 catgcgaggt atttgcatcc cttacccccg atttccatat ttctttttcc ataacacgac
     4381 cagagaacct gccaggccaa acaccagcag caccaccggg agcagcatca ggcatgacat
     4441 cagctggtcc tcgtacttta aaaataccgg cgttttgccg agcatgtaac ccagagttgt
     4501 caggatcaat acccacagca gaccgctcat ccagttgaaa aactgaaagc gcgcgttatt
     4561 cagccctgat aacccggcaa tcgtcggcag cagtgttctg acaaacgcaa taaagcgacc
     4621 aattaacagc gccgataaac cgtgtttatg aaaaagatgg tgtgcgcgtt gatgataatg
     4681 cgcgggtaaa tgagatagcc agttttgtac ggtgcgggta ttgcccagcc atcgcccctg
     4741 aatatagctg acccagcagc cgaggctggc ggcaacggtc agcagcagaa tcgtttgcgg
     4801 atagcccatc gcgcctttcg caatcaacac gccgaccaat accagtaaac tgtcgcccgg
     4861 taaaaaggcc gccggaagca agccgttttc aaggaacaaa attacaaaca agacaaaata
     4921 caacatgcca atcatcgatg gattggccag ggtttcaaaa tcctgcgccc acagggcttg
     4981 cagcaattgg gtcaaaagtt ccattcatta ttcctggtga atcggttaac gccacgctgg
     5041 ttttaaaaga tgcactaccg caatgttatt gctgttttgg tatgttctgc gggtttgata
     5101 catcaacaca gcctgaacta ttccctgtta aatcgtgaga taagaaagca ttcaaaaaca
     5161 taaattcaaa ttgaatctaa ttgtaacaaa ggtcaacgtt gttcgttatc aaaattacat
     5221 gtccgtggag tctgattttg ctgaatgcag gggagggtgg cgagaggatt tacacaggct
     5281 gacactttat acttttcgct taccgacgag acaaacaggc gcattgcagc gcctgtcgga
     5341 caggaatttc atttactgcc gctggtggct gtatcgagat gcaccaccgg gttttcggcg
     5401 aatagatagc ggtcggcatt gaactcgaag tcgtcgctgg tttcgttgaa taacatgatc
     5461 ttggtgtttt ccaggtgctg ccacattgcc agcttagcag catgaggatc tttgcgaatc
     5521 agcgccttga ggatttgatc gtggtcatca caccagttat cgacggtacg ggaatcaata
     5581 tgttcgtgca gttttttcca gtacgggtta tggctacgct gggtccacat tttttcaacg
     5641 atagccgcca gggcggagtt ctgcgtcgcc agggctacct gaatatggaa ctgcaaatcc
     5701 cactcggaat cacggaagca ttgttcaccg cgcgcctgct cctggatggc catcaatttc
     5761 atgatgtcct gtttcgttac ctgagttgct gcgaactcgg caatattact ttcgatgagt
     5821 tggcgagcct ggagtaactc aaacggaccg taattggcga attccatgtt attgtccgca
     5881 gcctgctgat ggcgcggctg gttggaaacc acgtgaatac ctgaaccttt acgcacttcg
     5941 acatagcctt caacttccag catgatgatg gcttcacgaa ctaccgtacg gctgacgttc
     6001 ttttcatcgg caataaagcg ctcggcaggc agtttatcac ccaccagata gacgccctgt
     6061 tcgatgcgct ctttcaggtc agcggcaagt tgttgataca aacggcgtgg ttcagtgatt
     6121 tccatatgcg ctccaggcat gatacggcag actatatttg ttataccact tttgcgagtt
     6181 tagctctact cgcagtgatg aaaaagccgc ctgagaaggc ggctttgaag ggaaactaaa
     6241 acttacacga agcgttaatg ttgcggcgtg ggatcgttat gtgtttcctg cgccacctca
     6301 atcgccggtt tgttctgcaa cacggtccag ataaccagcg cacccagcag gtcaaatact
     6361 gccagaactg cgaacagcgg gctgaagccg atggtgtcag ccagtgcacc gacaaccagc
     6421 gcaaacagcg tacttgccag ccatgcggac atcccggtta aaccgttcgc cgtcgccact
     6481 tcgttacgac cgaacacgtc agaagagagc gtaatcagcg cgccagacag tgcctggtga
     6541 gcaaaaccac cgatacacag cagcataatt gcgacatacg ggttggtgaa caaaccgatc
     6601 atacccgggc caatcatcag cactgcaccc agcgttacga ccatcttacg ggaaacgatc
     6661 aggttcacac caaaccaacg ctggaacagc ggcggcaggt aaccaccgag gatacaaccg
     6721 aggtcagcaa acagcatcgg catccaggcg aacatcgcga tctctttcag gttaaagccg
     6781 taaactttaa acatgaacag cgggatccac gcgttaaaag taccccaggc tggttctgcc
     6841 aggaaacgcg gcagcgcgat accccagaac tgacggttac gcaggatctg accaacggac
     6901 attttcttcg ccgtgctcac ctggtgctgg gcttcctgac cattaataat atagtcgcgt
     6961 tcttcatcgg tcagatgctt ctggtcgcgc ggatgtttat agaaaatcag ccatgccatc
     7021 gcccagataa agctcaatgc accggagatg ataaatgcca tctgccagct gtgcatcacg
     7081 attgcccata ccaccagcgg cggcgcaatc atcgcaccaa tcgaagaacc tacgttaaag
     7141 tagcctactg cgatggaacg ctctttcgcc gggaaccatt cggagctggc tttcagaccc
     7201 gccgggatca tcgctgcttc cgcggcaccg accgcaccac gagcaacagc caggccaccc
     7261 cagctacctg ccagcgcggt tgcaccacag aacacggccc acagcacagc aaacattgca
     7321 taaccgattt tcgtacccag cacatccagt acataacccg ctaccggctg catgaccgta
     7381 taagcagcag aataggctgc gatgatatag gaatactgtt gggtggagat gtttaactct
     7441 tccatcagag ttggcgcagc tgccgccaca gtgttacgcg tcaggtaacc aagcacggtg
     7501 ccgagcgtca ccagtgcgat catataccaa cgtaaccctt taattttacg catgtaaaac
     7561 ctcatcgttt tgttatatcc gcaggaagcg cggccatcat cccgccagag gctgaagatg
     7621 ttagttacga ccgggtaacc gaaaaaaaac cggcacgccc cgaacgttgc catgaaagtt
     7681 tcgtgctaat tcggcttccg taccggttaa cccgattcat ctaacaaatg agtgaatcgg
     7741 caatttgcat tccgtcgact tatgatttgc gacggcagaa agataacttg tcatacaact
     7801 ttaaaaggtg agagccatca caaatgtgcg aatatttgta gggacattac ctgacgacag
     7861 cgaggccagt actggcgcgg cctgcagcga gatttaccac tttaagagta atttttttaa
     7921 ctacgtttat tgatctaact cacgaaaata tcttcggact ctggaaattg gtgtgataac
     7981 tttgtcagca tcgcaccata agcaagctag ctcactcgtt gagaggaaga cgaaaatgac
     8041 tccgtttatg actgaagatt tcctgttaga taccgaattt gcccgccgtc tgtatcacga
     8101 ctacgcaaaa gaccagccga ttttcgatta ccattgccat ttgccgccgc agcagattgc
     8161 ggaagactat cgttttaaaa acctgtatga catctggttg aagggcgatc actacaaatg
     8221 gcgcgctatg cgtaccaacg gtgtggctga gcgtctgtgt actggtgatg cgtctgaccg
     8281 tgaaaaattt gacgcctggg cggcgactgt tccgcacact atcggcaacc cgttatacca
     8341 ctggacgcac ctcgaactgc gtcgtccgtt tggtatcact ggcaaattgc tttctccgtc
     8401 aactgccgat gaaatctgga acgaatgtaa cgaactgctg gcgcaggata acttctccgc
     8461 gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc accaccgatg acccgatcga
     8521 ttctctggag catcacgcag agatcgccaa agacggctct ttcaccatca aagtgctgcc
     8581 gagctggcgt ccggacaaag cctttaacat cgaacaggcg acctttaacg actacatggc
     8641 gaagctgggc gaagtttccg ataccgacat tcgccgcttt gctgacctgc aaactgcctt
     8701 gaccaaacgt ctggatcact tcgccgctca cggctgtaaa gtgtctgacc acgcgctgga
     8761 tgtggtgatg tttgctgaag cgaacgaagc ggaactggac agcattctgg cgcgtcgtct
     8821 ggctggcgaa accctgagcg agcacgaagt ggcacagttc aaaactgcgg tgctggtgtt
     8881 ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag taccacattg gcgcgctgcg
     8941 taataacaac ctgcgtcagt tcaaactgct ggggccggat gtaggctttg actccatcaa
     9001 cgaccgtccg atggcggaag agctgtctaa gctgctgagc aagcagaacg aagaaaacct
     9061 gctgccgaaa accattctct actgcctgaa cccgcgcgat aacgaagtgc tgggcaccat
     9121 gatcggtaac ttccagggcg aaggtatgcc gggcaaaatg cagttcggtt ccggctggtg
     9181 gtttaacgat cagaaagacg gtatggaacg tcagatgacc caactggcgc agctcggttt
     9241 gctgagccgc tttgtcggta tgctgactga cagccgtagc ttcctgtcat acacccgtca
     9301 cgaatacttc cgccgcattc tgtgccagat gatcggtcgc tgggtggaag cgggcgaagc
     9361 accggcggac atcaacctgc tgggcgagat ggtgaaaaat atttgcttta acaatgcgcg
     9421 tgactacttc gccattgaac tgaactaagg tctggggttg atatgcaata catcaagatc
     9481 catgcgctgg ataacgtcgc ggtcgcttta gcagatttgg cggaaggcac agaagtcagt
     9541 gtcgataacc agactgttac gctgcgccag gatgttgctc gtggacataa atttgcgtta
     9601 acggatatcg caaaaggggc caacgtcatc aaatatggcc tgccgattgg ttatgcattg
     9661 gcggatattg cggcggggga acacgttcac gcccacaata cgcgcacgaa tctgagcgat
     9721 ctggatcagt atcgctatca acctgatttt caggatctgc ctgcgcaagc ggcagatcgt
     9781 gaagtgcaga tctatcgtcg cgctaacggc gatgtcgggg tgcgtaatga gctgtggatc
     9841 ctgccaaccg tgggctgtgt caacggcatc gcgcggcaga tccagaaccg tttcctgaaa
     9901 gagaccaaca acgccgaagg taccgacggc gtgttcctct tcagccacac ctacggctgc
     9961 tcacagctgg gcgacgatca cattaatacc cgcaccatgc tgcaaaacat ggtgcgccac
    10021 ccgaacgcag gcgcagtgct ggtgattggc ctgggctgtg aaaacaacca ggttgccgca
    10081 ttccgtgaaa cgctgggcga tatcgatcct gaacgcgttc atttcatgat ctgccaacag
    10141 caggatgatg agatcgaggc cgggatcgag catttgcatc agttgtataa cgtgatgcgc
    10201 aacgataaac gcgagccagg caaactcagc gaactgaagt ttggtctgga gtgcggtggt
    10261 tctgacggtc tttctggtat tactgctaac ccgatgctgg ggcgtttctc tgactacgtg
    10321 attgctaacg gcggtactac cgtactgacc gaagtgccgg agatgtttgg cgcagagcag
    10381 ttgctgatgg accattgccg cgacgaagca acgtttgaaa aactggtcac tatggtcaac
    10441 gacttcaaac agtactttat tgcccatgac cagccgatct atgaaaaccc atcgccgggg
    10501 aacaaagcgg gcggtatcac cacgctggaa gacaaatcac ttggctgtac ccagaaagcg
    10561 ggttccagcg tcgtggttga cgtgctgcgt tacggcgagc gtctgaaaac gccggggctg
    10621 aacttgttaa gtgcgccggg taacgatgcc gtagcgacca gcgccctggc gggtgcgggc
    10681 tgccatatgg tgctgttcag tactggtcgt ggcacgccgt atggtggatt tgtgccgacg
    10741 gtgaaaatcg ccaccaacag tgaactggcg gcgaagaaaa aacactggat cgactttgac
    10801 gcgggtcagc tgatccacgg taaagcgatg ccgcagttgc tggaagaatt tatcgacacc
    10861 atcgttgagt ttgccaacgg taagcaaacc tgtaacgagc gtaacgactt ccgcgaactg
    10921 gcgatcttca aaagcggcgt aacgctataa agtcgaaatg agttgttaat aaaaaacggc
    10981 gtttcataat gtgaagcgcc gtttgctttt ccatacctgc taaggattta tcatgaccgc
    11041 gtattggctg gcccagggcg tgggtgtcat cgcctttctg attggtatca caacattttt
    11101 caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc tatagcgccg ttattggcgt
    11161 acattttttt cttctgggca cctatcccgc tggtgccagt gccatcctta atgccattcg
    11221 tacattgatt accttacgca cgcgcagctt atgggtaatg gcgattttta ttgtgctgac
    11281 tggcggaatt ggcctcgcga agttccatca tcctgtcgaa ctattgccgg ttatcggtac
    11341 gattgtcagt acctgggcgc tgttccgctg taaagggctc accatgcgct gcgtgatgtg
    11401 gttttcaacg tgttgctggg tgattcacaa cttctgggcg gggtcgatag gcggcacgat
    11461 gattgagggt agttttctgc ttatgaatgg cctgaatatc atccgtttct ggcggatgca
    11521 gaaaagggga attgatccat ttaaagtaga gaaaacccct cccgccatag acgaaagggg
    11581 ttaaacaatt aattacgcag ggcgctattt gccagacgat cgtcttctgc ctggcaagct
    11641 gccgcagtga atagcacgtc agttgaagag ttcagcgcgg tttcgcaaga gtcctgcaat
    11701 acgccgatga taaagccgac ggcaaccacc tgcatggcga tatcgttcga aataccgaac
    11761 atattacagg ccagtgggat cagcagcaga gacccccccg ccacgccgga tgcgccacag
    11821 gcacacagag aagcaactac gctcaacagc agcgctgtgg gcagatcgac cggaataccc
    11881 agcgtattaa ccgcagccag cgtcaacacg gtaatggtga ttgctgcgcc cgccatattg
    11941 atggtggcac ccagcggaat agaaacggaa taggtatcgc gatccagatt cagcttttca
    12001 cacagcgcca tattcaccgg aatgttagct gcagagctgc gggtgaagaa ggcatacacg
    12061 ccgctttcgc gcaggcacag cagcaccagc gggaacgggt tacgacgaat tttccaccac
    12121 accagcaatg ggttaaccac cagcgccacc agtaacatac agccaaccag cacgaccagc
    12181 agttgcgcgt agccccacag tgtggagaaa ccggtggttg ccagggtaga agaaaccagc
    12241 ccaaaaatac cgatcggtgc gaagcgaatg accagtttca ccataaaggt gacggcattc
    12301 gacatatcgt tgaccaggtt tttggtggtt tcgttaccgt gacgcagtgc gaaaccgagg
    12361 ccaattgccc acaccaggat cccgatgtag ttacctttca gcagcgcgtc aatggggttg
    12421 gaaaccatgc tcattaccag tccacgcatc acttcgacaa tgcctgacgg cggcgaaata
    12481 tcacccgcgc tactggacaa gtgcagggta gaagggaagg caaagctgaa gactactgcg
    12541 gccagagctg cagagaaggt gcccagcaga tagaggaaca aaataggacg gatattggtt
    12601 ttctgcccgt gctggtggtt agcaatagat gccattacca gcatcaacac caggatgggg
    12661 gcaacagctt tcagtgcgcc gacgaacaaa gtacctaaca gaccaacagc ttccgccgcg
    12721 ggttttgaga tccatgccag aagaatcccc agaacaaggc cgaccaggat ttgttttacc
    12781 aggctgccat gagccagacg ccggaatagc cccggtgaac gttgcgtagt catttttcga
    12841 tcctttcatt gtgttgttgc acatccctgg tgcgttctgt cgacgcatct ttcaggatgt
    12901 aacaaagtgt ttgcaaggtc gagtataagg aaagttacag cttcgggaag cgaaaaatgc
    12961 tggattttaa agagaggtca tgttttgcaa cttaatggtt acatttattt gacataaaca
    13021 gagggaagga ctggctacta aggctgatta gataacatat gggcaaaact atttggaata
    13081 tgagaacggt tgcgctcctt taatgtgtta tgccatataa agtcactggc gcagggggcg
    13141 gccaatcgcc gccgccccct gcacccccag gctctggcga acaaaatcgc cgctgcgcgg
    13201 ttccctcagc ttatcccttg cggctaccgg gtcggcctcc agggaacgct tcggcgattt
    13261 tgacgccacc aacacccgtg cggtcttttt gtaaagagct ataagccgat tacataatta
    13321 atagctcacc tttgtctgga atgagttatc tctagtaatg gcttaatata cagcgaatca
    13381 ttctccccgc tgcttatcat gccgataatt cacccaggcg ttgataataa acgtcatcac
    13441 caggatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga agtcgacaat
    13501 cagcatcttg ataccgataa acaccagaat caccgccagg ccatatttga gcatcgagaa
    13561 acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga tcgcaaacag
    13621 gtttgaggtc agcacaatga acgggtcagt ggtcacggcg aagatagccg gaatgctatc
    13681 cacggcaaaa atcacgtcgc tcaattccac cagaatcaat accagcatca gcggtgtggc
    13741 gtacaacaag ccattcttgc gcacaaagaa atgctcattg tcgatggtgt cggtcatgcg
    13801 caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt catcttcatg
    13861 ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat acaggatcca
    13921 gtcgaactgc gaaatcagcc agctgcctgt gaagatcatg atggtacgca gaacaatcgc
    13981 cccgagcacg ccatacacca gcacgcgacg ttgtaatgcc gccggaacgg agaaatagct
    14041 gaacaacatc agccagacaa agacgttatc gaccgccagc gatttctcaa tcagataacc
    14101 cgtgagaaag gccagtgcct gtggatcggc aacggcgcga ccttcggttt gcaccagata
    14161 ccaccagaaa gcggcgttaa acagtaacga cagcgtcacc cagaccagcg accaggccgc
    14221 agcctgtttc atggtcatgg catgtgcccc acgacgcccc tgcaacaaca ggtcgatagc
    14281 cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc cgacagtatt
    14341 catagaagtt ccttacacat aaaaaaaacg gccaacgtcg gaagacgtca gccgctgctt
    14401 tttatgcata gacctcgcct tccggcaagg tctcacttac aacaaaaaag gaatacgtct
    14461 gcgtattcct ttcgggttgc ccggtgaccg gatgtggttt ttcacacatc gtaatgacga
    14521 tcgaccggca atgaagttac tcccctttgc aggtaacaaa gtatgacaga ccattcagtc
    14581 agtcaatggt ctgttacata ccttttacac tgttttactt tggcaattat agttttacgc
    14641 tatctgccgg aaaaatcacc ccagtctggc ggcggatctc ggtcagcagt ttggctgtga
    14701 tgcgactgac cgccagccct ggatgatcca ccagatgctc atccaccagg gtagcgaaca
    14761 gctctgcttc gtagagcatg gtattaatat gctgcggctg ggtgagatcc tgcatctggc
    14821 tgccacgcgg cacgaagcac actttctggc attcagacag tttttcaatc accagcgatc
    14881 ccgcttcgcc ctgaatctcg cttgccagga cagaatcact gacctttgag tgctgcaagg
    14941 tgacgctgaa atcaccgtaa tccatcacca ccacgccgtg ggcatctaca ccacttgcca
    15001 gtaaactggc ggttgcctgc acgcttttcg gctcaccaaa taacgctacc gccgacgcca
    15061 ggcagtaaaa gccgatatcc ataattgaac cgttagagaa tgccggatta aaggtgttgg
    15121 gattctcacc atccaggtaa cgttgatacc gcgaggaata ctggcaatag ttgaaaaaga
    15181 ctttccgcag tttgcctact ttcggcagcg cctggcgcag caaatgaaag ttcggcaggc
    15241 tggctgtttt aaatgcctca aacagcacca cctgattttc ccgcgcacag gcaatggcgg
    15301 catccacttc cgccagattc gacgccagcg gtttctcgca aatcacatga attttgtgac
    15361 taaggaaaag ttgtgcctgg gaaaagtgca gggaattcgg gctggcaata tacaccgcgt
    15421 caatggcatc gctttccgcc atcgcttcca gcgaggtaaa cagatgctcg acagaaaaat
    15481 cattggcgaa gtgctgagcc tgctcaaggc tgcgggaata aacggcggtt aacttgtatt
    15541 taccgctctc atgggcggcc tcgacgaact ggcgagtgat ccagttcgta ccaatcacag
    15601 cgaaacgtat cataacgcgt ggatactcca taaagggatt ctgtcgtcaa tgtagcacgc
    15661 ctttttcatc ttcgacgtgc gcagatacgc attattcttt cgccggacag ccatgctcac
    15721 taaacggccc aatcatgacc gatggcccga gatatttcgg ctggcgatgg aggattttgg
    15781 cgtcaatcac cgcgctgaca cgagcgagtt tctcccgcag ttgagtataa aaaccgtgct
    15841 tcatattcag gtctggcgcg ttaaacccga tagcgtcgat gccgtactgt tttgccagcc
    15901 agatggcgcg ttggttatga aattcttgcg agataatggt gatatggttt tccccaaaca
    15961 cctttttggc acgcactacc gaatccagcg ttgagaatcc ggcatagtcg cagaagatca
    16021 ctttcgccgg tacgccttta gcgatcaaag cctgctgcat acctgatgct tcatcgtaat
    16081 ttttacgtcc gttatcgcca ctgactaaca gccatttcac ttttccggcg tggtacaact
    16141 ctgctgcggt atcaatacgt cgggtaaagt agcgattacc cggcctggcc cccagcaata
    16201 agcccacgtt acgcgccgga acgacgttga cgtcgctcca ggtcagctgt ttgctggcgt
    16261 tgaccattac gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac agtaagcagg
    16321 caatctttag agtccggcga gaaaaacaaa tacggagaag aaggcgggca aatgcgcgca
    16381 gcatggtcgt tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaagaaaa
    16441 gataacgcct gacatcattt tcagattttg tcctgcccaa gcgacaactg ggttagccac
    16501 agtcgggtgt cgaactcaag ctggtggtac tggggttcca tatgacagca caactggtaa
    16561 aacgctttgt tatgctcttt ctccttcagg tgcgccagct cgtgcaccac gatcatgcgc
    16621 aaaaacggtt ccggcgcgtt gcgaaacacg gtagcgacgc ggatctctgc ttttgctttc
    16681 agcttgccgc cctgcacacg agaaacagcg gtatgtaacc cgagcgcgtt ttttagcaca
    16741 tggatcttat tgtcatacat cactttattg atcggcgggg cattgcgcag aaactgattt
    16801 ttcagatcct gagtatattg ccagagggct ttatcggtgg cgtagtcgtg tgttcccgga
    16861 tagcgttttg ccagcacatc gcccagacgc tgttcgttaa tcagtgttcg cacctgggaa
    16921 agtagctgct cgggataacc ctgaagataa gtaagattgc tcataaccgc ccacgaaaaa
    16981 gagaaaaagg gtatactcac gcaccctttt caggggcaat gtcgaaattt tatcattcag
    17041 gagggccgat gagccactta gacaacggtt tccgttcact gacactacaa cgttttccgg
    17101 cgacggatga cgttaacccg ctacaggcgt gggaagcggc ggatgaatat ttgctgcaac
    17161 agttggacga cacagaaatc cgcggcccgg tgttgatcct gaatgatgcc tttggtgcgt
    17221 taagctgcgc gctggcggaa cataagccgt acagcattgg cgactcatac atcagtgaac
    17281 tggcgacgcg cgagaattta cgcctcaacg ggattgatga gtcgagcgtg aagtttctcg
    17341 acagcaccgc cgactacccg caacagccgg gtgtggtgct gatcaaagtg ccgaaaacac
    17401 tggcattgct ggaacagcaa ctgcgtgcgc tgcgcaaagt ggtcacgtcg gatacacgta
    17461 ttattgccgg tgccaaggcc cgtgacattc acacttccac gctggaactg ttcgaaaaag
    17521 tgctcggccc gaccaccacc acgctggcat ggaaaaaagc gcgcctgatt aattgcactt
    17581 tcaatgaacc gccgctggcc gatgcgccgc agaccgttag ctggaagctg gaaggtactg
    17641 actggactat ccacaaccat gcgaatgtct tctcccgcac cgggctggat attggcgcgc
    17701 gcttctttat acagcatctg ccagagaatc tcgaaggtga gattgtcgat ctcggttgcg
    17761 gtaatggcgt tattggtctg acgctgcttg ataaaaatcc gcaggcgaaa gtggtgtttg
    17821 tcgatgaatc accgatggcg gttgcttcca gccgtttgaa cgttgaaacc aacatgccag
    17881 aggcgttgga tcgctgcgaa tttatgatca acaacgcgct ctccggcgtg gagcctttcc
    17941 gctttaatgc tgtgctctgc aacccgccgt ttcatcagca acatgcgctg accgataacg
    18001 tcgcctggga gatgttccac cacgcccgcc gctgcctgaa aatcaacggc gagctgtata
    18061 tcgttgccaa ccgtcacctg gattacttcc ataaactgaa gaaaattttc ggcaactgca
    18121 ccaccatcgc cacgaataat aaatttgtgg tgctgaaagc agtgaagctg ggacgtcgtc
    18181 ggtaagatta gcagctacct gccggataag gcgtaaacgc cttatccggc atacgttctg
    18241 cacgttaccc ccatgtcgga taagacgcgg caagcgtcgc atccggcaat gtctgcccag
    18301 ttctggaatc gcgctccata aaacaaaaaa tccctgtcac catcctctac attctcttgt
    18361 ttagcgttgt tcaacgttta tgctacatac gagaaggttt gacgcataaa gaatacgatt
    18421 tctttaccgc tgttcatcgt actaagggga aaaaatgatt acgattgccg acatcttgca
    18481 agcaggagaa aagctaactg ctgtggcacc ttttctggcg ggtattcaga acgaggaaca
    18541 atacatccag gcgctggaac tggtagatca tctgctgctc aacgatcctg aaaacccctt
    18601 actggatctg gtgtgtgcca aaataaccgc gtgggaagaa tcagcgcccg aatttgcgga
    18661 atttaatgcc atggctcaag ccgtgcctgg cggtatagcc gtgattcgta cccttatgga
    18721 tcaatatggt ttaacccttt ccgatctgcc ggaaattggc agtaaatcta tggtgtcacg
    18781 cgttttgagc gggaagagga aattaacgct ggaacacgct aaaaaattgg caacgcgatt
    18841 cggcatttct cccgccttgt ttatt
//
LOCUS       DAZUCE010000086        18853 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZUCE010000086 DAZUCE010000000
VERSION     DAZUCE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.086"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            49..918
                     /locus_tag="ACHY03_002523"
     CDS             49..918
                     /locus_tag="ACHY03_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFS3905737.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1111..1464)
                     /locus_tag="ACHY03_002524"
     CDS             complement(1111..1464)
                     /locus_tag="ACHY03_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFS3905738.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1602..3248)
                     /gene="groL"
                     /locus_tag="ACHY03_002525"
     CDS             complement(1602..3248)
                     /gene="groL"
                     /locus_tag="ACHY03_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFS3905739.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3292..3585)
                     /locus_tag="ACHY03_002526"
     CDS             complement(3292..3585)
                     /locus_tag="ACHY03_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFS3905740.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3861..5117
                     /gene="yjeH"
                     /locus_tag="ACHY03_002527"
     CDS             3861..5117
                     /gene="yjeH"
                     /locus_tag="ACHY03_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFS3905741.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5133..5609)
                     /locus_tag="ACHY03_002528"
     CDS             complement(5133..5609)
                     /locus_tag="ACHY03_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFS3905742.1"
                     /translation="MRWLPFIVIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5946..7382
                     /gene="aspA"
                     /locus_tag="ACHY03_002529"
     CDS             5946..7382
                     /gene="aspA"
                     /locus_tag="ACHY03_002529"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFS3905743.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7500..8801
                     /gene="dcuA"
                     /locus_tag="ACHY03_002530"
     CDS             7500..8801
                     /gene="dcuA"
                     /locus_tag="ACHY03_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFS3905744.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8917..9255
                     /gene="cutA"
                     /locus_tag="ACHY03_002531"
     CDS             8917..9255
                     /gene="cutA"
                     /locus_tag="ACHY03_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFS3905745.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9231..10928
                     /gene="dsbD"
                     /locus_tag="ACHY03_002532"
     CDS             9231..10928
                     /gene="dsbD"
                     /locus_tag="ACHY03_002532"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFS3905746.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEANGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKYLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10965..11540
                     /locus_tag="ACHY03_002533"
     CDS             10965..11540
                     /locus_tag="ACHY03_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3905747.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11647..11722
                     /locus_tag="ACHY03_002534"
     tRNA            11647..11722
                     /locus_tag="ACHY03_002534"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11680..11682,aa:Phe,seq:gaa)
     gene            complement(11918..12955)
                     /locus_tag="ACHY03_002535"
     CDS             complement(11918..12955)
                     /locus_tag="ACHY03_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3905748.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPIRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYVDVCMESAAQTLEDALSLYTDERT
                     VSKICKGGNKS"
     gene            complement(13023..14504)
                     /locus_tag="ACHY03_002536"
     CDS             complement(13023..14504)
                     /locus_tag="ACHY03_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFS3905749.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGRWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPLAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVQSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESSIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFF
                     QARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            complement(14497..15987)
                     /locus_tag="ACHY03_002537"
     CDS             complement(14497..15987)
                     /locus_tag="ACHY03_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFS3905750.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARARPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDANPVTTALVLGQKCRVVLVGDC
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKCHAREHLQGHYTVLSRTVAGVIATALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRREYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPIVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRSLRTLVLNELMHQLAEEQQKTEGLRGTGYDNSGRVQENGHA"
     gene            16219..17583
                     /locus_tag="ACHY03_002538"
     CDS             16219..17583
                     /locus_tag="ACHY03_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3905751.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITETRARTEG
                     DVAALAAARAHSDENDKRTLVSANTYADQKETDINNRTDGLLATERQARTAGDAATLA
                     AARTHSDENNKRTLISANTYADQKETDINNRTDGLLMTERQARIAGDAETLATAEAYS
                     DAGDSLTLKQATAYSDISTRRALSEAYRYTDNKFRNLSNKIERAEKRLNAGIAGVTAI
                     SSIPYVAENSFSYGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGV
                     AGGW"
     gene            complement(18509..18751)
                     /locus_tag="ACHY03_002539"
     CDS             complement(18509..18751)
                     /locus_tag="ACHY03_002539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905752.1"
                     /translation="MMMARDITAYHLNSVHISARRFPEIFQWAIGMQTKNLLLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
BASE COUNT         4705 a         5045 c         4433 g         4670 t
ORIGIN      
        1 taaattaaaa ttaattattt aattgagaaa tggttaggga gaacctacat ggccataagc
       61 atcaagggtg ttaataccgg tgttattcgc aagagtaata atttcattgc actggcgctg
      121 aaaattaaag aacctcgcaa taaagaatcg ttgttcttta tgtctgtcat ggaactgcgc
      181 gacctgctga ttgcgctgga aagtcgtctg catcaaaagc ataaactgga tgctgctgca
      241 cgccttcagt atgagcaagc tcgcgataaa gtcattaaga aaatggcaga aaatatccca
      301 gaaattctgg ttgatgagct taaaaatgcc gatattaatc gtcgggttaa cacgctggaa
      361 cttaccgata atcaaggcga aaatcttacc ttcgtattaa cgctacatga cggtagcaaa
      421 tgtgagttag tcgtcaatga gttgcagatt gaaatgctgg cgcgagcaat cattcatgca
      481 attaataatg cagaaatgcg cgaactggca ttacgtatta cctcactgtt agatttcctg
      541 ccgttatacg acgttgactg ccaggaaaat ggcaatctgg aatatgacac ctattcccaa
      601 ccggaatgga aacataactt gtttgaccat tatctggcgg tgctttaccg ctttaaagat
      661 gaaagtggca aagaacaatt tagcggcgca gtggtgaaaa cgcgtgaagc aacgccgggt
      721 aaagaagtag aggcgatcac tcgccgaatg cttgatttca gcccacgact gaaaaaactc
      781 gcgggtgtgc catgtcaggt ctatgttcgc accgtagcgg cgaataacgc ccaaccgcta
      841 acccaggatc aatgcttacg cgcgctgcat cacttacgtg ttcaatccac cagcaaaact
      901 gcaccacagg cgaaataatc tcatctttgc taatgcgagg ctggagccat ccagcctgaa
      961 atgactgata acgtgatcgt tggtcatgcc ggatgcggcg taaacgcctt atccggccta
     1021 caaattctca agcattcaat cagttgcaat gccttatgta ggcctgataa gcgaagcgca
     1081 tctggcaatt atacgtttgt caccatcaca tcagttcggg cacttataaa cctgaccgat
     1141 aatctggcta tccgtcggga caaaactgga caacattccc tgcgacgggc tgctgatgcc
     1201 ataaatcacg ttaccgccca ttgcagccgc ctggttgcgc agatcgtttg cagcgccgcg
     1261 catagaaccg ccctcttctc cgtgttgccc ggaaagccag ttgctttgct tacctgtcgc
     1321 agtaccaatc agctggcact ctgcgcctgg ctgctcgtcc acaatgcgta cactctgacc
     1381 ggcagcactc aattcgttgc tggagctaca acccgccatc agtagcgcgg caccgacaat
     1441 ccctgctaag tattttacgt gcatgttatt ccccataatc aatgagctgg acgccactcg
     1501 cgtcgtccgt gtctgaatct tatactagaa agatgaccaa aagaaaaacc cccagacatt
     1561 tctgcccggg ggtttgttta tttctgcgag gtgcagggca attacatcat gccgcccatg
     1621 ccacccatgc cgcccatacc gccagcagcg cctaagtcag ctgcatcgtt tttcggcagg
     1681 tcggtaacca tgcattcggt ggtgatcatc aggccagcca cagaagctgc gtactgcaga
     1741 gcagaacgag ttactttggt tgggtccagg atacccatgt cgatcatgtt gccgtattct
     1801 tcggttgctg cgttgtaacc gtagttgccg tcgccacctt taacggtgtt agcaacaaca
     1861 gacggttctt cgccgcagtt caggacgatc tgacgcagcg gagcttccat tgcacgcagt
     1921 gcaactttga tacccacgtt ctggtcttcg ttctgaccac gcaggtcagc cagtttagac
     1981 gctacgcgga tcagcgcaac accaccacca gcaaccacgc cttcttctac cgcagcacgg
     2041 gtcgcgtgca gggcgtcttc aacgcgtgct tttttctctt tcatttcaac ttcggtagca
     2101 gcacctactt tgataactgc aacgccgcct gccagtttcg ctacgcgctc ctgcagtttt
     2161 tcacggtcgt agtcagaagt tgcttcttca atctgctgac ggatctgagc aacacggccc
     2221 tggattgcag cttcttcacc cacgccatcg atgatagtgg tggtgtcttt gttgatcaca
     2281 acacgtttag cctgacccag gtcttccagg gttgcttttt ccagctccat accgatctct
     2341 tcagagatta cggtaccgcc agtcagggtt gcgatatcct gcagcatagc tttacgacga
     2401 tcgccgaagc ccggagcttt aactgcagca actttcacga tgccacgcat ggtgttaaca
     2461 accagagttg ccagcgcttc gccttctaca tcttcagcga tgatcagcag cggtttgcct
     2521 gctttggcaa cggcttccag aaccggcagc atttcgcgga tgttggagat tttcttgtca
     2581 gccagcagga tgaacgggct ttccagttct actgcgccag tttccggctt gttgatgaag
     2641 taaggagaca ggtagccacg gtcgaactgc ataccttcaa ccacgtccag ttcgtcctgc
     2701 agaccggtac cgtcttcaac ggtgataacg ccttctttac cgactttgtc catcgcttca
     2761 gcgatcagtt tacctacggt ttcgtcggag ttagcggaga tagtaccaac ctgagcaatc
     2821 gctttagagt cagagcacgg tacggacagc gctttcagtt cttcaactgc agcggtaaca
     2881 gctttgtcga taccacgttt caggtccatc gggttcatgc ccgcagcaac agctttcaga
     2941 ccttcagtga tgatagcctg agccagtacg gttgcagtgg tggtaccgtc gcctgcagcg
     3001 tcgttcgctt tagaggcaac ttctttcacc atctgcgcac ccatgttttc gaacttgtct
     3061 tccagttcga tttcacgagc aacggaaaca ccatctttgg tgatggtcgg tgcaccgaaa
     3121 gatttatcca gaactacgtt acggcctttc ggaccgaggg taactttcac tgcatctgcc
     3181 agtacgttta cgccgcgcag cattttcaca cgagcgtcgt taccgaattt tacgtcttta
     3241 gctgccatta tctttattcc ttaaattcgt atgttcagtg tcgtgcgagg attacgcttc
     3301 aacaattgcc agaatgtcgc tttcggacat gatcaacact tcttcattgt cgatcttctc
     3361 ggatttcaca ccgtagccat cgttgaaaat aacgatgtcg ccaactttca catccagcgg
     3421 cttcacttcg ccattttcaa ggatacggcc attgccgaca gccagcactt cgccgcgggt
     3481 ggatttagcc gctgcagagc cggtcagaac gatgccgcca gcagatttag tttcaacttc
     3541 tttacgcttg acgatcacgc gatcatgcaa tggacgaata ttcattgata actctccttt
     3601 gagaaagtcc gtatctgtta tgggtgacgc cggagcttac gtggtttccc ggctggtgac
     3661 cagagaaatg gggatgaggc ttcgcccctt caagggggaa aaagaaaaaa aattctgatc
     3721 agcacaaaat cgggtgaaaa ccctgattca cctcacattt catcgcaatt tcttcatcgc
     3781 cgtcataagc gaatctgatt gtgctaccat cgaaaatcta cgcagttgcc caaaatttgg
     3841 gcgcaatcgg acatcaaccc atgagtggac tcaaacaaga actggggctg gcccagggca
     3901 ttggcctgct atcgacgtca ttattaggca ctggcgtgtt tgccgttcct gcgttagctg
     3961 cgctggtagc gggcaataac agcctgtggg cgtggcccgt tttgattatc ttagtgttcc
     4021 cgattgcgat tgtgtttgcg attctgggtc gccactatcc cagtgcaggc ggcgtcgcgc
     4081 acttcgtcgg tatggcgttt ggttcgcggc ttgagcgagt caccggctgg ctgtttttat
     4141 cggtcattcc cgtgggtttg cctgccgcgc tacaaattgc tgccggattc ggccaggcaa
     4201 tgtttggctg gcatagctgg caactgttgt tggcagaact cggtacgctg gcgttggtgt
     4261 ggtatatcgg tactcgcggt gccagttcca gtgcaaatct acaaacagtt attgccggac
     4321 ttatcgtcgc gctgattgtc gctatctggt gggcgggcga tatcaaacct gcgagtatcc
     4381 ccttccccgc gccaggaaat atcgaactta ccgggttatt tgctgcgtta tcagtgatgt
     4441 tctggtgttt tgtcggtctt gaagcatttg cccatcttgc ctcggaattt aaaaatccag
     4501 aacgtgattt tcctcgtgct ttgatgattg gtctgctgct ggcaggatta gtctactggg
     4561 gctgtacggt agtcgtctta cacttcgacg cctatggtga acaaatggcc gcggcagcat
     4621 cgcttccaaa aattgtagtg caactgttcg gtgtaggagc gttatggatt gcctgcgtaa
     4681 ttggctatct ggcctgcttt gccagtctca acatttatat acagagcttc gcccgcctgg
     4741 tctggtcgca ggcgcaacat aatcctgacc actacctggc acgcctctct tctcgccata
     4801 tcccgaataa tgccctcaat gcggtgctcg gatgctgcgt ggtgagcact ttggtgattc
     4861 atgctttaga gatcaatctg gacgctctta ttatttatgc caatggcatc tttattatga
     4921 tttatctgtt atgcatgctg gcaggctgta aattattgca aggacgttat cgactactgg
     4981 cggtggttgg cgggctgtta tgcgttctgt tactgacaat ggtcggctgg aaaagtctct
     5041 atgcgctgat tatgctggcg gggttatggc tgtttctgcc aaaacgaaaa acgccggaaa
     5101 atggcataac cacataatcc ggcgtttcga cattaatcct ggcgatcgtc tttatgatca
     5161 aggcggtcgc ggtcatcatc ctttcgctgg tactcaccat caaaagtatt accgccaccg
     5221 gtcccggcgc taaaaccgcc gcctggcatg cgagaaaagc gcaaatgtgg catcaacttc
     5281 actgtcagat gcttttgcac cggcggcaat aaaagcagaa gaccgaggaa gtcggtgaaa
     5341 aagcccggta ataaaagcag caaaccggca atgatcagcg aaacactttt aatcatctcc
     5401 gccgcagggt tttcacccgc cgccattttt tgctgcatca gcacgaaatt cttaaagccc
     5461 tggttacgta ccagtgacat accgataact gacgtaaata taaccagcac gagagtcagc
     5521 aataccccca atacatgggc aacctgaata aagattgaaa tctcaatata gacataaagg
     5581 aaaatgacaa taaaaggtaa ccagcgcaaa ggtttctcct gtaatagcag ccggttaacc
     5641 ccggctacct gaatgggttg cgaatcgcgt tcagcttata ttgtggtcat tagcaaaatt
     5701 tcaagatgtt tgcgcaacta tttttggtag taatcccaaa gcggtgatct atttcacaaa
     5761 ttaataatta aggggtaaaa accgacactt aaagtgatcc agattacggt agaaatcctc
     5821 aagcagcata tgatctcggg tattcggtcg atgcagggga taatcgtcgg tcgaaaaaca
     5881 ttcgaaacca catatattct gtgtgtttaa agcaaatcat tggcagcttg aaaaagaagg
     5941 ttcacatgtc aaacaacatt cgtatcgaag aagatctgtt gggtaccagg gaagttccag
     6001 ctgatgccta ctatggtgtt cacactctga gagcgattga aaacttctat atcagcaaca
     6061 acaaaatcag tgatattcct gaatttgttc gcggtatggt aatggttaaa aaagccgcag
     6121 ctatggcaaa caaagagctg caaaccattc ctaaaagtgt agcgaatgcc atcattgccg
     6181 catgtgatga agtcctgaac aacggaaaat gcatggatca gttcccggta gacgtctacc
     6241 agggcggcgc aggtacttcc gtaaacatga acaccaacga agtgctggcc aatatcggtc
     6301 tggaactgat gggtcaccag aaaggtgaat atcagtacct gaacccgaac gaccatgtta
     6361 acaaatgtca gtccactaac gacgcctacc cgaccggttt ccgtatcgca gtttactctt
     6421 ccctgattaa gctggtagat gcgattaacc aactgcgtga aggctttgaa cgtaaagctg
     6481 tcgaattcca ggacatcctg aaaatgggtc gtacccagct gcaggacgca gtaccgatga
     6541 ccctcggtca ggaattccgc gctttcagca tcctgctgaa agaagaagtg aaaaacatcc
     6601 aacgtaccgc tgaactgctg ctggaagtta accttggcgc aacagcaatc ggtactggtc
     6661 tgaacacgcc gaaagagtac tctccgctgg cagtgaaaaa actggctgaa gttactggct
     6721 tcccatgcgt accggctgaa gacctgatcg aagcgacctc tgactgcggc gcttatgtta
     6781 tggttcacgg cgcgctgaaa cgcctggctg tgaagatgtc caaaatctgt aacgacctgc
     6841 gcttgctctc ttctggtcca cgtgccggcc tgaacgagat caacctgccg gaactgcagg
     6901 cgggctcttc catcatgcca gctaaagtaa acccggttgt tccggaagtg gttaaccagg
     6961 tatgcttcaa agtcatcggt aacgacacca ctgttaccat ggcagcagaa gcaggtcagc
     7021 tgcagttgaa cgttatggag ccggtcattg gccaggccat gttcgaatcc gttcacattc
     7081 tgaccaacgc ttgctacaac ctgctggaaa aatgcattaa cggcatcaca gctaacaaag
     7141 aagtgtgcga aggttacgtt tacaactcta tcggtatcgt tacttacctg aacccgttca
     7201 tcggtcacca caacggtgac atcgtgggta aaatctgtgc cgaaaccggt aagagtgtac
     7261 gtgaagtcgt tctggaacgc ggtctgttga ctgaagcgga acttgacgat attttctccg
     7321 tacagaatct gatgcacccg gcttacaaag caaaacgcta tactgatgaa agcgaacagt
     7381 aatcgtacag ggtagtacaa ataaagaagg cacgtcagat gacgtgcctt ttttcttgtg
     7441 agcagtaact taaaaataac aatctaatat caacttgtta aaaaacaagg aaggctaata
     7501 tgctagttgt agaactcatc atagttttgc tggcgatctt cttgggcgcc agattggggg
     7561 gaataggtat tggttttgca ggcggattgg gggtgctggt tcttgccgct attggcgtta
     7621 aacccggtaa catcccgttc gatgtcattt ccattatcat ggcggttatc gccgctattt
     7681 ctgccatgca ggttgctggc ggtctggact atctggttca tcagacagaa aagctgctgc
     7741 gccgtaaccc gaaatacatc acgatcctcg caccgatcgt gacctatttc ctgactatct
     7801 ttgctggtac tggcaacatc tctctggcga cactgccagt tatcgctgaa gttgcgaagg
     7861 aacaaggcgt taaaccttgc cgtccgctgt ctactgcagt ggtatccgcg cagattgcga
     7921 tcaccgcatc gccaatctca gcggcagtgg tttacatgtc ttccgtgatg gaaggtcatg
     7981 gcatcagcta cctccatctg ctctccgtgg tcatcccgtc caccctgctg gcggttctgg
     8041 tgatgtcctt cctggtcact atgctgttca actccaaact ctctgacgat ccgatttatc
     8101 gcaagcgtct ggaagagggc ctggttgaac tgcgcggtga aaagcagatt gaaatcaaat
     8161 ccggtgcaaa aacgtccgtc tggctgttcc tgctgggcgt agttggcgtg gttatctatg
     8221 caatcatcaa cagcccaagc atgggtctgg ttgaaaaacc gctgatgaac accaccaacg
     8281 caatcctgat catcatgctc agcgttgcaa ctctgaccac cgttatctgt aaagtcgata
     8341 ccgacaacat cctcaactcc agcaccttca aagcaggtat gagcgcctgt atttgtatcc
     8401 tgggtgttgc gtggctgggc gatactttcg tttccaacaa catcgactgg atcaaagata
     8461 ccgctggtga agtgattcag ggtcatccgt ggctgctggc cgtcatcttc ttctttgctt
     8521 ctgctctgct gtactctcag gctgcaaccg caaaagcact gatgccgatg gctctggcac
     8581 tgaacgtttc tccgctgacc gctgttgctt ctttcgctgc ggtgtctggt ctgttcattc
     8641 tgccgaccta cccgacgctg gttgctgcgg tacagatgga tgacacgggt actacccgta
     8701 tcggtaaatt cgtcttcaac catccgttct tcatcccggg tactctgggt gttgccctgg
     8761 ccgtttgctt cggcttcgtg ctgggtagct tcatgctgta atgaccaatc gcggggcgtt
     8821 cacgccccgc tttctttccc gccgactaac atccttcccc cgtccgttgt atagtgacct
     8881 ctctcttgcg gttccatctg ttcttgcgag gtgtttatgc ttgatgaaaa aagttcgaat
     8941 accgcgtctg tcgtggtgct atgtaccgca ccggatgaag cgacagccca ggatttagcc
     9001 gccaaagtgc tggcggaaaa actggcggcc tgcgcgacct tgatccccgg cgctacctct
     9061 ctctattact gggaaggtaa gctggagcaa gaatacgaag tgcagatgat tttaaaaact
     9121 accgtatctc accagcaggc actgctggaa tgcctgaagt ctcatcatcc atatcaaacc
     9181 ccggaacttc tggttttacc tgttacacac ggagacacag attacctctc atggctcaac
     9241 gcatctttac gctgatcctg ctactttgca gcacttccgt ttttgccgga ttattcgacg
     9301 cgccgggacg ttcacaattt gtccccgcgg atcaagcctt tgcttttgat tttcagcaaa
     9361 accaacatga ccttaatctg acctggcaga tcaaagacgg ttactacctc taccgtaaac
     9421 agatccgcat tacgccggaa cacgcgaaaa ttgccgacgt gcagctgccg caaggcgtct
     9481 ggcatgaaga tgagttttac ggcaaaagcg agatttaccg cgatcggctg acgcttcccg
     9541 taaccatcaa ccaggcgagt gcgggagcga cgttaacagt cacctaccag ggctgtgctg
     9601 atgccggttt ctgttatccg ccagaaacca aaaccgttcc gttaagcgaa gtggtcgcca
     9661 acaacgcagc gtcacagcct gtgtctgttc cgcagcaaga gcagcccacc gcgcaattgc
     9721 ccttttccgc gctctgggcg ttgttgatcg gtattggtat cgcctttacg ccatgcgtgc
     9781 tgccaatgta cccactgatt tctggcatcg tgctgggtgg taaacagcgg ctctccactg
     9841 ccagagcatt gttgctgacc tttatttatg tgcaggggat ggcactgact tacacggcgc
     9901 tgggtctggt agttgccgcc gcagggttac agttccaggc ggcgctacag cacccatacg
     9961 tgctcattgg cctcgccatc gtctttacct tgctggcgat gtcaatgttt ggcttgttta
    10021 ctctacaact cccctcctcg ctgcaaacgc gcctcacgct gatgagcaat cgccaacagg
    10081 gcggctcacc cggcggagtg tttattatgg gggcgattgc cggactgatc tgttcaccat
    10141 gcaccaccgc accgcttagc gcgattctgc tgtatatcgc ccaaagcggg aacatgtggc
    10201 tgggcggcgg cacgctttat ctctatgcgt tgggcatggg cctgccgctg atgctaatta
    10261 ccgtctttgg taaccgcttg ctgccgaaaa gcggcccgtg gatggaacaa gtcaaaaccg
    10321 cgtttggttt tgtgatcctc gcactgccgg tcttcctgct ggagcgagtg attggtgatg
    10381 tatggggatt gcgcttgtgg tcggcgctgg gtgtcgcatt ctttggctgg gcctttatca
    10441 ccagcctaca ggccaaacgc ggctggatgc gcgtggtgca aataatcctg ctggcagcgg
    10501 cattggttag cgtgcgccca cttcaggatt gggcatttgg tgcgacgcat accgcgcaaa
    10561 ctcagacgca tctcaacttt acacaaatca aaacggtaga tgagttaaat caggcgctcg
    10621 ttgaagccaa cggcaaaccg gtgatgttag atctttatgc cgactggtgc gtcgcctgta
    10681 aagagtttga gaaatacacc ttcagcgacc cgcaggtgca aaaagcgtta gcagacacgg
    10741 tcttacttca ggccaacgtc acggccaacg acgcacaaga tgtggcgctg ttaaagtatc
    10801 ttaatgtcct tggtctaccg acaattctct tttttgacgg acaaggccag gagcatccac
    10861 aagcacgcgt cacgggcttt atggatgctg aaaccttcag cgcacatttg cgcgatcgcc
    10921 aaccgtgaac gacacttgca gtgggaaaga cggaggaaaa tagcgtgcaa cgtgaagatg
    10981 tactgggaga agccctgaaa ttattagaat tacaagggat tgccaacacc acgctggaga
    11041 tggttgctga acgtgtggat tatccactgg acgagctgcg ccgcttctgg ccagacaaag
    11101 aggcaatcct ctacgatgcg ctgcgttatc ttagccaaca gatagatgtc tggcgtcgtc
    11161 agctgatgct ggacgaaacg caaaccgccg aacaaaagtt gctggcacgt tatcaggcgt
    11221 tatcggagtg cgttaaaaac aaccgctatc cgggctgtct gtttatcgct gcctgtacgt
    11281 tttatcccga tcctggccac cctattcatc aactggccga tcagcaaaaa agcgcggcct
    11341 acgatttcac ccacgaactg ttaaccacgc tggaagttga cgatccggcg atggtagcaa
    11401 agcagatgga actggtgctg gaaggctgtt taagccgaat gctggtgaat cgtagccagg
    11461 cggatgtcga cactgcacat cggctggcgg aagatatcct gcgcttcgcc cgctgccgcc
    11521 agggtggcgc attgacctga cagaaacaca gaaaagaagc gatttgccgc aatcttaagc
    11581 agttgaatcg cttttactga aattaggttg acgagatgtg cagattacgg tttaatgcgc
    11641 cccgttgccc ggatagctca gtcggtagag caggggattg aaaatccccg tgtcggtggt
    11701 tcgattccgc ctccgggcac catataccag aacacgctgg ttaagggatg agatccttga
    11761 cgggcggtta atacaggatg cggcatcaaa gggtctccgt ttgtgtaagt tgtggttaca
    11821 ctgattgagt ttggtatcgt atcctcaaac agggtgtctt ttccggagac gccctgttct
    11881 cgttaacact gtatgcttac atcgctgatt cagcctgtca agatttattc ccccctttgc
    11941 aaatcttgct cactgtgcgc tcatctgtat acaaactcag ggcatcttcc agtgtctgcg
    12001 ctgcagactc catacagaca tcaacatacc ccatggtcgt tgtaagactc ctgtgtccca
    12061 gtatggattt gaccagatgt aaattacgtt caggatattt catcagtctg gttgccagcg
    12121 tatgtctgaa acgatgtggt gaaacaggaa aaccacattc tcgcgaaagt cgccggaaaa
    12181 atgaacgtac ggtctgatgc gcccgaatgg gacagtactg atatctgtcg gcagagatcg
    12241 gctcactgaa acgacagacg tcaaaaagat agtcatcatc acctgccccg cactgacggg
    12301 cccgctccag cagtttttcc atcgatacct tcaggggagc caccagagga acgccccatt
    12361 ccctgtgagt tttacttccc tccagcttaa gatcaattat gctttcatcc ggacagacat
    12421 ccctcacacg gatatgcttc agttgattaa agcgcattcc ggtataacgc agaacatcca
    12481 gaactgtcag ccagtaccag gcagggtata tagcattgcg tcgacgacta ttcaccatgt
    12541 tctgctcttc cgcttctgca aacctctgca caaccaggta aaccctgagc atctgattgt
    12601 cactcagtgt ctttttcttt ttttcagtac tcgcaactga tgcttccacc aacgggtttt
    12661 ctgtctgagg cagcagtttc atcttgatcc cgaatcccat aattgcccgc aagtgggcca
    12721 ccttgttatt ccaggtcacc tccctgcgct tctgctcttt caggacataa cgccggtatc
    12781 gctgaacatc acggacagtc acctcccccg gcaaaatatc accgataaat tccctgaata
    12841 ccctgacaac tttgcagtag ctccactctg tatccggccg aagattcttt gaaaaaaagt
    12901 actcttccag cagttgttcc caggtgattt ttgtgacgga atcgtcattc tgcattatat
    12961 attccccttg tttactgtgc tcccgcactc tccggtcaaa ttctgcacat catcgccttt
    13021 actcacccga aaacaataag cgggctgtct ccgggaaccg taatattccc gtacagaaac
    13081 ctgcccttaa ccagatatcc cgaaaccatt ttgtaacgtc cggaatgttc tgcgtcacta
    13141 taaagacggg cctgaaaaaa acgctctcct gaacgtacct tatggatacc tgcccgttca
    13201 aagagcgact gaacataacg acgatcctgc ccggaaccac tctccttcag aaaacgataa
    13261 aaaatccccg gggcacacag aaaaacatat ccggaaacaa catgaacatt tgaatcaacg
    13321 ctgttaaccg aaagtgaacc ctccctcagc ccctctttta accatctgat aaattcactc
    13381 tctaccctgc tatcctctgt cagggaaaac tctgccgttt tttccactgt ttccgggata
    13441 cggtttccct gattttctgt tacacatccg ctctcttccg gtaactcaat actgctttct
    13501 ttatccggcg gcacgcctga cgctatctgc cctccctcat ccgacaaatc agcagccgaa
    13561 aacagcgtaa ggagcattcc ggtatcagca tcagtttcgt tcccctcatc cacctcctgt
    13621 tccgctgtcc ggccctcatt ttcggcatcc tccactggcg actgtacaac cggcaacggc
    13681 tgaataacgc tttctgctga aattgctgct gactgcagca gtaccggaac aacctcagcc
    13741 accggaactt cggccacatc tcctgcagcg aggggactca cgacttcctc catagcttcc
    13801 tgtaatactg tacgtatcag cgccatttca ggatgctgat gccagactgc tccggcaaga
    13861 ttctgtaatg cccccgggac agtggcaagc caggctcccg cctctccggg cagcagttgt
    13921 cccgccatca gggctgccag ttgccgtccc tccgttccct ggcagggcat cttcctgaac
    13981 cggaaccggt atacgccctc cggtacagat atccccggct gccagcatct gccgtcggcc
    14041 tgttcgcctt ccagttccgc cagcaacggc aggtgataaa acagagccga ccagaacact
    14101 accatgttcc acacactccc ctgcgcggcc tgctcttctg ctgtcgctcc cggtggaaac
    14161 attctgcctc tggccagccg gacagcatac gtcgtgaact gcagggtcag gtcaccaaaa
    14221 ccaccggtgt aaccccagcg accttctgct gtggccggca gattctgact gacagacaac
    14281 aactgatgca caggatcaag acaaaggcgc tgatacatat ccaccggcat ggaactgttt
    14341 tcccataact gtttcagaca ccttttcctg aacggtgtgg ataccagtac atcagccggc
    14401 aatggtgtaa aatacccctc cggcaccgtt tccccgggaa tatccgtccg gacatcagaa
    14461 cattctgtct tcacaggaaa taaccttttc aggcgttcaa gcatgcccat tctcctgaac
    14521 ccgacctgaa ttgtcgtacc cggtcccccg cagaccttca gttttctgct gttcttctgc
    14581 cagttgatgc atcagctcat tcagtaccag cgtcctgagg gaccgggtga cagccacata
    14641 taacagacag gtttcatcat gacgctcagc ctccgtcatc agcggatccg tgatatcctg
    14701 gaaatcctca tccagcatta caatcggcca ctccagcccc ttgctccggt gtgccgttga
    14761 caccgtgata tctgcatcct gctcattcgt taccgtatac tgccggagaa gttccagttt
    14821 tttcggtaac ggaaaacaca actccagaag ccgaagggac tgattcatct ccacatcctt
    14881 cgtggatttt gcaatatatt tatattcttc aaaattcctg tactcccggc gcagcctgtc
    14941 tgaatgcatc cgttccggca tatcaacctg gaaccagtag agatcctcaa gttccccggt
    15001 cctgtaacct tcgattcccc ccacccagta aaccttttgc cctttcattg ccgccatcag
    15061 cgcagtagca atcacacccg cgacagtacg actgagtacc gtgtaatgcc cctgaagatg
    15121 ttcacgcgca tggcatttca gcaatacctc atcatcacca ccacaacctt tcacctctcg
    15181 tgtttcacct tcacgctgca gaagcagatt tgccatacga gccaccgccg gaccaaaacg
    15241 gaaactctgt gtcagccaca accggtccgc attcttcagt gcaggatggg aaagcgcatt
    15301 attcgcaccc cgaaagcggt aaatctgttg ataacaatct ccaacaagga ccacccgaca
    15361 cttctgcccc agaaccagag cagtggtcac cggattcgca tcctgcgcct catcaaacag
    15421 gatggtgtcc catcggtgag acaggtctgc accggacaac tggaagagtt tcagataagt
    15481 atcgtgcgtc accgggaacg tccccccggg gttgctcatt tcgttccaga gcaactgcac
    15541 cgcccgaagc gcatctgctg gcggaacacc ccgcatctca tccttatccg gcaggtgatg
    15601 caggcccggt accgcatcgg cagaacagat aaagtgattc agccccgtcc ctgccacccg
    15661 tgccagagcc cagtatcgtg tattcagctt tctcgccaca tcagtaatac gtaatcccgg
    15721 aaccagccga tgacggtagt gtcttccaac ccgggcatac gccagctgat gtgacgttct
    15781 gcattccacg ttaaagggaa atttacgctc tgcctcatca cgtattgccc ggttataggc
    15841 aagatagagc atacggctgt ccggacgggc ccgggcaaac tgcaccagcg tactcgtctt
    15901 ccctgtgccg gaaaacgcat tcacaaccag ttgctgcccc tgccagttaa taatggcgta
    15961 ctgttcatct gtgggattaa aagacatgca ttctcttctc cggaaaaaca tttccgtaat
    16021 ctgatgcatc aggggtatgc ccaccacaaa taactctgta cagcaacaga aaaatttccc
    16081 tggtcatatg atgaattaat catcgccatc acaccggatg cttctcatcc tgtcctcaga
    16141 aaggcaggtg gtctcctgtc tgccgcgctt atccaggata ccgggacctg aaaggtcata
    16201 atctggcagg aaaaaataat gtccgaagta aaaaaacacg tttcttattc attaacagtt
    16261 gcagctatgc tgtcagccgc catcgtctgt cagacagcac atgctgatgt caacacaccg
    16321 aacatcaccg gagaaaaaaa caatacaaca acacgattct ccggaaatat ttacggcagt
    16381 gaaaatactg tcacatcaga gtcaaacagt ctggttgtgg gtaacagtaa ttccgtggag
    16441 ggagggtata acaacattgt tattggtaat tcctccacgg tcaaagcttc cggtttgatc
    16501 agaaaagtgg gcgaagatat tgtgctggat ggttacagcg tcagcctcgg taatgccacc
    16561 agaattaacg gtgattcatc tgttgccata ggtctgacat cgtccgtaac cgggaacgac
    16621 tccgtggcac tgggcagtca ttccagtgct aacggaaaca atatcgtatc cgtcggctct
    16681 gacacactga aacgccgcat cacaaatctg gcacagggga aagatgacca tgatgcggta
    16741 aatctgatgc agatgaccag tgctgttaac cgggaagctg gagaaagaat aaaatcaaca
    16801 aacaatctgc agaatcagct taataccctc tctggtcagg tcataactga aaccagggcc
    16861 cgaacagaag gtgatgtagc agcgctggct gcagccagag cccacagcga tgaaaacgac
    16921 aaaagaacac tggtcagtgc gaatacttat gctgaccaga aagaaaccga catcaataac
    16981 cgtactgacg gattactggc tacagaacgc caggcacgca ccgcaggcga tgctgcaact
    17041 ctggctgctg ccagaacgca cagcgatgaa aacaacaaaa gaacactgat cagtgcgaat
    17101 acctatgctg accagaaaga aaccgacatc aataaccgta ctgacggatt actgatgacc
    17161 gaacgccagg cacgcatcgc aggtgatgca gaaactctgg caacagcaga ggcatacagt
    17221 gacgccgggg attcactgac actgaaacag gcaacagcct acagcgatat cagtacccgc
    17281 cgggcactgt cagaggccta tcgttatacc gacaacaaat tcagaaacct gagcaataaa
    17341 attgaacggg cagaaaaaag acttaatgca ggcatcgccg gagtgacagc aatatcctcc
    17401 atcccctatg tggctgaaaa ttctttttca tacgggatcg gcgtgggtaa ctatcagaac
    17461 gctaatgctg tggccgcagg cattcagtat aaaacatcac gtaataccaa tatacgcctc
    17521 aacatctcct gggactcatc tcataacacc gcgattggtg caggggttgc cggcggctgg
    17581 taaataatct tgcgtacata cggcattatg gtattccgta atcacattaa ttcagtacgc
    17641 aacacagccc catccggaac agaccactgg actgagcggg ataatcaagg aaaaagggcg
    17701 cgcagtgtgg tcagccttca ctacaatccg gcggcagatt tcctgccgcc atgttgcccg
    17761 taaacattta agttcgcagg ctaacattat tgccacaagc cccgtatttc cctggtgaat
    17821 cctcaaagcg agtccctcac catccagaca aaacacagta tcgtcgatta ataatcacta
    17881 aaagcgttcg tcgtagcatc ataacccctg aatgaacaca tccctgtttg caaggattct
    17941 gttgttctga aatcgacgag tagctgcaac tctgtatccg cccttattgc agtcagtgca
    18001 gtctggccac gatgcgattc gctgacctga gccttattct ccaggcggac tttatgcaat
    18061 gctcccctga aatcttccat tattttacat gtacaggcat catattaaat atggaaataa
    18121 taacgaacga aaaagggaca caatgaatat caaagcacca gtataaacca ggatgttttg
    18181 aacaatttct tactctcctg aatacacact gtaaatcatc agaaactgca ctagccatgc
    18241 taagtacact ctgctacgaa ctgatgagga agtgcggtat caattacctg gacagcctgt
    18301 tcaacggaga ggtatatcta ttatctatgt aattacaggg ggactgatat cagcgaaaaa
    18361 gcaggtcagc atattcacta cccctggtct gaactgaaac aacgtcagcc agaaataagc
    18421 ctataatgcc tcatataagt ggataacttt tcatccgtcg ttgacatcat acacatgaaa
    18481 cattgataac agtcatgaat acaccgaatt atctttctga ctcagcgttt ccagtcagta
    18541 gcatttcttc cggtaaaggc atttccgaaa cgtcacgttc gccattaaat acaaaaatac
    18601 tttctgcgta atcattctgt aacaaagaat tagttaacag cagatttttt gtttgcatac
    18661 cgatggccca ctgaaaaatc tcggggaaac ggcgggcact gatgtgtaca gaattgaggt
    18721 ggtacgcagt aatatctctt gccatcatca taagatggca catcatatcc cgaacgcaaa
    18781 cctggcacat gatgaacaga cggaaggctc agcaggactt ctggataatt atacacgata
    18841 gccagttact gct
//
LOCUS       DAZUCE010000087        18807 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZUCE010000087 DAZUCE010000000
VERSION     DAZUCE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.087"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..303)
                     /gene="trbJ"
                     /locus_tag="ACHY03_002540"
     CDS             complement(<1..303)
                     /gene="trbJ"
                     /locus_tag="ACHY03_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFS3905753.1"
                     /translation="MLPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGVATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWILVSEIYYSRMTGLNES"
     gene            complement(233..787)
                     /gene="trbB"
                     /locus_tag="ACHY03_002541"
     CDS             complement(233..787)
                     /gene="trbB"
                     /locus_tag="ACHY03_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFS3905754.1"
                     /translation="MSLTKSLLFTLLLSVAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     DTSARTDKTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLALQYG
                     FSVFPYTLDGQGDTAFPQALPAPPDVLKTFFPNIPVATPTTFLVNVNTLEALPLLQGA
                     SDAAGFMARMDTVLQMYGGKKGAK"
     gene            complement(774..1055)
                     /gene="traQ"
                     /locus_tag="ACHY03_002542"
     CDS             complement(774..1055)
                     /gene="traQ"
                     /locus_tag="ACHY03_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001732192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFS3905755.1"
                     /translation="MSKYKFSLPRLDITGVWVFSLGLWFHIVARLVYRKPWMAFFLAE
                     LIAAILVLFGAYQILDTWIARVSREEREALEARQAAILAAEKESRHVSH"
     gene            complement(1121..1522)
                     /locus_tag="ACHY03_002543"
     CDS             complement(1121..1522)
                     /locus_tag="ACHY03_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905756.1"
                     /translation="MDKENSSIPERWLIASGVAVILLTLLAFMSFPEPDVSMNRYYSW
                     LMNVLILSVTGVMYVALSVVMVILYFLYERNSRLLYLSVLALIIAAGIWLLRFLDNFL
                     MTPAFHVDLFVLWVLLCFLYIRCKNEMSIPE"
     gene            complement(1653..2018)
                     /locus_tag="ACHY03_002544"
     CDS             complement(1653..2018)
                     /locus_tag="ACHY03_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905757.1"
                     /translation="MNFISLWPLLLAQVTVMSLTVIFLAADRRFFSWDCFVVCSLSMM
                     MAEGIVIMKYELWHIPPALLGLAGVLSWLLLLLHTGYFSGISEGVHKRLWLAVASVFS
                     GAGMIIFYWLLQPSETIMK"
     gene            complement(2032..2775)
                     /gene="traF"
                     /locus_tag="ACHY03_002545"
     CDS             complement(2032..2775)
                     /gene="traF"
                     /locus_tag="ACHY03_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFS3905758.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKMKQPEPENKPAPVI
                     PRQEPDIMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEQYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(2768..3025)
                     /locus_tag="ACHY03_002546"
     CDS             complement(2768..3025)
                     /locus_tag="ACHY03_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFS3905759.1"
                     /translation="MKVIFTSNRLVDFLIRLLITAIVISPVIIWSWDTVKETTAGGVL
                     AAAFIILYSGVLLFILYFCFSALADLQKPDERKSDERNEDE"
     gene            complement(3039..4889)
                     /gene="traN"
                     /locus_tag="ACHY03_002547"
     CDS             complement(3039..4889)
                     /gene="traN"
                     /locus_tag="ACHY03_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFS3905760.1"
                     /translation="MKRLLPLLLGLAVSVAQADSNSDYRAGADFARQIQGQGIGSIQS
                     FNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWATSETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCSRT
                     ARITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPVAGTVESASLSAHAGLYFL
                     NSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLTGSGCTANGNCLPHGNGDR
                     MVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLKGTECSEPGGTKT
                     GVMEGKTWSITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGACLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAHSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKSPDCRGIT
                     VDELQRIKFDQLDFTNFYEDLMNNQKIPDNGALTEKVKEQIAGQLKQVGQ"
     gene            complement(4886..5308)
                     /locus_tag="ACHY03_002548"
     CDS             complement(4886..5308)
                     /locus_tag="ACHY03_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFS3905761.1"
                     /translation="MALTKKQREKLRMKFGGRCAYCGCELPEKGWHADHVQAVLRKSE
                     QCMKAAEKRIFRLKSTGDVFRPEADCPENLVPACAPCNLLKTTYSLEMFRTQVSLQVE
                     RGRRSSVNFRTAERFGLIQVVDKPVVFWFEQYQRESEV"
     gene            complement(5334..5705)
                     /locus_tag="ACHY03_002549"
     CDS             complement(5334..5705)
                     /locus_tag="ACHY03_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905762.1"
                     /translation="MSLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTTLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLTWGWSVKGTFNSDFQLCFRLQPEGPHHWY
                     HRYCRRSLRADFRTLVRLVNP"
     gene            complement(5702..6340)
                     /gene="trbC"
                     /locus_tag="ACHY03_002550"
     CDS             complement(5702..6340)
                     /gene="trbC"
                     /locus_tag="ACHY03_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFS3905763.1"
                     /translation="MSRKIFVLFALTTGLFISAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKATAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVVATGDCRQVAQGLLDGTGDKSQ"
     gene            complement(6367..6888)
                     /locus_tag="ACHY03_002551"
     CDS             complement(6367..6888)
                     /locus_tag="ACHY03_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905764.1"
                     /translation="MGFFSFLRKISDKEKGVFISEENWFSLLEKVSAFDKVRQWAESN
                     GGAYYPLQECRRLTDIEDIRKSEDILNRLEFIDRRFREFISIMSEYNCNDKSKDRWEK
                     WLSEYPPEKIYQVRREGDHLSLKNYGDDITLDRDTAVATVQITVSRKCITQEEMDELY
                     ALSERLKEKCAGN"
     gene            complement(7293..8285)
                     /gene="traU"
                     /locus_tag="ACHY03_002552"
     CDS             complement(7293..8285)
                     /gene="traU"
                     /locus_tag="ACHY03_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFS3905765.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWSCIFP
                     LSLGSTKVSQGKVPDTANPSMPLQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPEFGKTAQGTAKKEDKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNTIGVDVAVCYEYPSPILP
                     KARWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(8282..8914)
                     /gene="traW"
                     /locus_tag="ACHY03_002553"
     CDS             complement(8282..8914)
                     /gene="traW"
                     /locus_tag="ACHY03_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFS3905766.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKARVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            complement(8911..9297)
                     /gene="trbI"
                     /locus_tag="ACHY03_002554"
     CDS             complement(8911..9297)
                     /gene="trbI"
                     /locus_tag="ACHY03_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFS3905767.1"
                     /translation="MTTTQKTTDVTARQRSHWWWTVPGCLAMVLLNAAVSYAIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKKLSEVQSKALADRFNAALEASLQTWQQKHHAVILVS
                     PAVVQGTPDITREIQQDIARRMRAEP"
     gene            complement(9294..11924)
                     /gene="traC"
                     /locus_tag="ACHY03_002555"
     CDS             complement(9294..11924)
                     /gene="traC"
                     /locus_tag="ACHY03_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFS3905768.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEILGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTLGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAETQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLALVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLTRQNRARIDDVVDFLKNARDNDQYAESPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(12051..12398)
                     /locus_tag="ACHY03_002556"
     CDS             complement(12051..12398)
                     /locus_tag="ACHY03_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905769.1"
                     /translation="MKLIYLKYFLSFVFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTIGKANKIASECRKRNEMAEARRVRENERNKIREKQ
                     LQALNDAHSVKER"
     gene            complement(12558..12797)
                     /locus_tag="ACHY03_002557"
     CDS             complement(12558..12797)
                     /locus_tag="ACHY03_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905770.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVQTNEPLNNVMETLNGGGQCLLLRRK"
     gene            complement(12877..13353)
                     /locus_tag="ACHY03_002558"
     CDS             complement(12877..13353)
                     /locus_tag="ACHY03_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905771.1"
                     /translation="MHKSENEKIDFILDEHERIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRRVLSRQELLWLMSLTEDCPDM
                     HQELLNRLLSGKKLTGLDKREIRSLWWEKMDAMQESATRQREQDTIRQFSEESKSE"
     gene            complement(13346..13567)
                     /gene="traR"
                     /locus_tag="ACHY03_002559"
     CDS             complement(13346..13567)
                     /gene="traR"
                     /locus_tag="ACHY03_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFS3905772.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(13702..14217)
                     /gene="traV"
                     /locus_tag="ACHY03_002560"
     CDS             complement(13702..14217)
                     /gene="traV"
                     /locus_tag="ACHY03_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFS3905773.1"
                     /translation="MKKISLFVPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKMEQPADAKPVAASLPRLADGNFRTTSVQAVAATTPSRSRPAVTALPEQKLQAPR
                     PLFTAAREVKTVVPVSSVVPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(14214..14486)
                     /locus_tag="ACHY03_002561"
     CDS             complement(14214..14486)
                     /locus_tag="ACHY03_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFS3905774.1"
                     /translation="MFISLMTEQSEITEIKMNMRNLNIIKSSPQSLLHNFNIAAVGLM
                     KKGMSMSLFSSPGHIMVVGQTCAGKANLTAELISAVVGNNKKGEGQ"
     gene            complement(14425..14985)
                     /gene="traP"
                     /locus_tag="ACHY03_002562"
     CDS             complement(14425..14985)
                     /gene="traP"
                     /locus_tag="ACHY03_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFS3905775.1"
                     /translation="MVGDALIFRVAFVVRWLLWVVRFVVIWPLATMALMALFVLWKDN
                     TTPGKLLVQEIDFVRQTAPAGQFPVRECLPSLHDLPGGAEKICRYRAADAADYVRETD
                     RSLMQLTTAFWATLALMYMSFAAGTGKYPMWPGKMKCVRVAAADERLKGVYTEDASLP
                     EKIRKCPVYFPDDRAIRNNGDKNEHA"
     gene            complement(14975..16429)
                     /gene="traB"
                     /locus_tag="ACHY03_002563"
     CDS             complement(14975..16429)
                     /gene="traB"
                     /locus_tag="ACHY03_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFS3905776.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGTGAVPPPTAFYPGNGTTPPPQVTYQSVPVPNRIQRKTFSYDADRKKGPSLPYIPSG
                     SFAKSVLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVS
                     SERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVD
                     GIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVI
                     PIGAGNEVTVVFQDGFQLKTVEEMALEQSQSQAEEGNAESPVPNPPTAESHLNGFNTD
                     QMLKQLGNLNPQQFMSGDRNQQGGDAGKEGGNGG"
     gene            complement(16429..17157)
                     /gene="traK"
                     /locus_tag="ACHY03_002564"
     CDS             complement(16429..17157)
                     /gene="traK"
                     /locus_tag="ACHY03_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFS3905777.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFVVENKTLSALNIRESDFWQSGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(17144..17710)
                     /gene="traE"
                     /locus_tag="ACHY03_002565"
     CDS             complement(17144..17710)
                     /gene="traE"
                     /locus_tag="ACHY03_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFS3905778.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLAVNVIQGINNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALAFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(17732..18043)
                     /gene="traL"
                     /locus_tag="ACHY03_002566"
     CDS             complement(17732..18043)
                     /gene="traL"
                     /locus_tag="ACHY03_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFS3905779.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAGICIGWGMMT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSFWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(18058..18423)
                     /gene="traA"
                     /locus_tag="ACHY03_002567"
     CDS             complement(18058..18423)
                     /gene="traA"
                     /locus_tag="ACHY03_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFS3905780.1"
                     /translation="MNAILSVQGASAPVKKKSFFPKFTRLNMLRLVRAVIPAAVLMMF
                     FPQLALAAQGAGQDLMAGGNATVKATFGKDSSAVKWVILAEVLVGAIMYMMTKNVKFL
                     AGFAIISVFITVGMAVVGL"
     gene            complement(18456..18683)
                     /gene="traY"
                     /locus_tag="ACHY03_002568"
     CDS             complement(18456..18683)
                     /gene="traY"
                     /locus_tag="ACHY03_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFS3905781.1"
                     /translation="MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVR
                     LRDHLSRFPDFYNLDAIKEGTGETDSIIKDL"
BASE COUNT         4637 a         5253 c         4281 g         4636 t
ORIGIN      
        1 tgattcattc agaccggtca tgcggctgta atatatttca gacaccaaaa tccatcccac
       61 aattgcccct acaaaaaaca caacagaaac agaaaaggta agacacagcg aaacgacaat
      121 ttccacaggg ctggcgggat aaaaaagccc ctgacggata aaggggatat tcaggctgac
      181 gattattccc gtcgcgacgc cggatattcc ggcaatgagt aacaccacct gcttatttcg
      241 cacctttttt tcctccgtac atctgcagaa cggtatccat ccgcgccata aaaccggcag
      301 catccgatgc gccctgtaaa agcggcagcg cttcaagcgt attgacgtta accaggaagg
      361 tggtcggtgt ggccaccggg atattcggga agaatgtttt cagtacatcc ggtggtgcag
      421 gtaacgcctg cgggaaggcc gtatcaccct gaccatccag ggtataggga aatacggaaa
      481 atccgtactg cagcgccagc tgcttcagta ccgggtcaaa ctggtgacag taagggcaat
      541 gcccctgcat aaacagcacc accttccagt cggccaggtt aacctgcctg ccgttgctga
      601 ggcggaacca gcggggagcc ggcttttctg tcttgtctgt cctggctgac gtatctgctg
      661 ccggttgtgc aggctgtgcg gccataccct gcggattcca gagtcgctct atttcatccc
      721 gggtggaggc ctgcacggca gcgactgaca gtaacagggt gaacagcagt gatttagtga
      781 gagacatgtc tgctctcctt ttctgcagca agaatggcgg cctgacgggc ttccagtgcc
      841 tcacgctctt cccggctgac gcgggctatc catgtatcca gtatctggta tgcgccaaac
      901 agaacgagaa tggccgcaat cagctctgcc agaaaaaaag ccatccaggg ctttctgtaa
      961 accagacggg caacaatatg aaaccagagc cccagggaga acacccatac acctgtaata
     1021 tcaaggcggg gtaaagagaa tttatattta ctcataaata tatcctgttt gtaatatctg
     1081 ccacactcag caggatggca aataaaaaac cgactgatta ttattccggt atactcattt
     1141 catttttaca gcgaatgtac aaaaaacaga ggagcaccca taaaacaaac agatcaacat
     1201 gaaatgctgg cgtcattaaa aaattatcca gaaatctgag aagccagata cctgctgcaa
     1261 tgattagggc aagcacagac aagtacaata acctgctgtt acgctcgtaa agaaaataaa
     1321 gtattaccat tacaacagac agcgccacat acattacccc ggtcacactc agaattaaca
     1381 cattcatcaa ccagctataa tagcgattca tactgacatc cggctccgga aaactcataa
     1441 aagccagtaa ggtcagtaaa ataaccgcaa caccagacgc gattaaccat ctttcaggaa
     1501 tactactatt ttctttatcc atacagaata aaaccatact taacaaacaa catgaattaa
     1561 aagccattca ccatcaggtg tggggacaga aaaaagttat ctctgattgt cttttactct
     1621 gtaaatgagt tcatttacat attaacaggg cattatttca ttatggtttc tgatggctgg
     1681 agtaaccagt agaatataat cattcctgca ccagaaaaca ctgaggcaac agcgagccac
     1741 aaacgcttat gcacgccttc tgatatgcct gaaaaatatc ctgtatgaag tagcagcagc
     1801 aaccatgaca acactcctgc cagacccagc agggcaggag ggatatgcca cagttcatat
     1861 ttcataataa caataccttc tgccatcatc atggacaggc tgcaaaccac aaaacagtcc
     1921 catgaaaaaa aacgcctgtc agcagcaagg aaaatcactg tcagtgacat cactgtcacc
     1981 tgggccagca ataaaggcca taaagaaata aaattcattt aaacgacctc tttaaaaatt
     2041 cggtttaaag tcttcagaaa cgttcaggaa ctgttttgcc aggtcgtcct gagaaataaa
     2101 gccgtatgat aacgggcgaa cactgccctg tttcgggtca accagcatca tggccgggaa
     2161 atatttcaca ccgaggcgct gcgcctgccc ctggtcagtc cgggaatccg gcaacagtgg
     2221 attaatcacg ccatccacgg aaacgggaat aacactcaga ccatacgtat cccggaagcc
     2281 attaatgacc tgcgccagct gcccgtcgat ggggtcctgc ccccggtaaa aaaacataat
     2341 gccgtattgt tcagccagtt tcgcaatggc ctgtcgctgc tgtgcctggt ctgccgccag
     2401 ctggttccgt accgtgccgt tgtaatggct gtactgcagg ttatagtcca gttcaggatg
     2461 tgccagcatg gcctttttgg cgctcatggt gaaaagcccg gcctgctgag tccagtaatt
     2521 ctgcagccgg aaatatttca caaaattatc cactccggga tacagaatgg cttcgtacag
     2581 cgcccgcttt gttgccgtct gcagcgcggt cagtttctgc ataatatccg gctcctggcg
     2641 gggaattaca ggtgcaggtt tattttccgg ttccggctgt ttcatctttt cgttatacca
     2701 ctgccagcct gcatctttcc ctgacgccgg aaaaataaag cagcagagta acagtggcag
     2761 taatgcttta ttcatcttca ttcctttcat cactttttct ttcatcaggt ttctgcaggt
     2821 cagccagtgc agaaaaacaa aaatacagaa taaacaggag cacaccggag taaaggataa
     2881 tgaaagcagc agcaagcacc ccgcctgccg tggtttcttt taccgtatcc catgaccaga
     2941 taattaccgg ggaaatgaca atcgctgtaa tcaaaagccg gataagaaaa tcaacaagac
     3001 gattcgacgt gaatatgact ttcattattt tcccgacctt actgccccac ctgtttcagc
     3061 tgaccggcaa tctgctcttt cactttttcg gtcagcgcac cattatccgg gattttctgg
     3121 ttattcatca ggtcttcgta gaagttagtg aagtccagct ggtcgaactt aatacgctgc
     3181 agttcatcca cggttattcc ccggcagtca ggactttttg cgctgccaaa cccgatacgc
     3241 aactgcccgt tacgcccctg ctgctggact atctgcgcca gcttcgaatc aaactggcag
     3301 taactgcgct ttttctgcag acagacaccc agcactttcc gggagcagaa ctcgcccaca
     3361 ctcaccgtca gtttgttgtc ctttgccttt cccagcgcct tttcttcgct gttgcacttc
     3421 gccagtccca catcctgacc ccagccacca tccttacagc agttactgaa gccggctgcg
     3481 gccttacggc agaatttcgc ctcgcccgtg aaagccctga catccacacc attcagggcg
     3541 gcaacatcct taccagcagc ggccagtgcc gccagctgtg atactgcctc accaaaatca
     3601 ctgctttttc cgctatgcgc cttgtcgcat tcaccgtcaa ggcagaagac atcaccacca
     3661 cagaccatca cttttccgga cgttctggac tcgcaggagt acgtggcata ctcatgcaga
     3721 caagctccct catcggaata aaacgcgcac tgacgtgagg ccagcgtaca ggccggatta
     3781 tcgacatatt tctgacaggt gccgttatct gcggactgtg tgacatattt atcccggtat
     3841 gcccagcacg cctgtgtgat gctccaggtt ttaccttcca tcacacccgt tttcgtacca
     3901 cccggttctg aacactcagt gcctttcagc acgccatccg ctttattaaa cggacaactc
     3961 tccacccact ccacccgtgg aacccattcc ttatcacgga ccttcatcct gagtttcagc
     4021 gtaaaggtgg aagcaccact gacaagcgat tcatagacca tcctgtcacc attcccgtgc
     4081 gggaggcaat taccgtttgc agtacagcca ctaccggtca gaacctgccc ctgtgtgaca
     4141 gaaaacccgg agggcacagg tatggtgaaa gtcccgctct gtcctgtaac cagttgcaca
     4201 ttaaaggctg tgttcataaa gtcgtaacgg gagttaagga aatatagccc tgcatgagcc
     4261 gacagcgagg cgctttccac tgtcccggca acaggtacgg taacgctgaa caccagtttc
     4321 agaccgttca tactgaaccg gaactgtgag tgcggtatcg tcacctcacg atattcatcc
     4381 gtgtatttcc agtctcctgt gatcctggct gtcctggaac aatattcctc caccatcgtg
     4441 tcacgttcac aggtatagtt tgtgaattca ctgcggttta tctgctgcgc actgcactgc
     4501 tgtccggtat ttcccacgat actgtcagcc cggttcacca catccctgcc ggtctgaata
     4561 aacggcgcat ccggtgacag aatgtctttc ggcttgttca tgaatgactc tgtgatggtt
     4621 ttgccggttt cactggttgc ccattgtgtg gtgccgttat ttttcagacc gccatccccg
     4681 ccggcagtca ccccaccgta atactgcgtc tcatccgggt tcgcgttata cccggggata
     4741 ctctcctgtg gattaaaact ctgaatactg ccaataccct gcccctggat ctgacgggca
     4801 aaatcagcgc cggcacggta atcactgttg ctgtcagcct gtgccacact gactgccagc
     4861 cccagcagca atggcagaag acgtttcata cctcactctc cctctgatat tgctcaaacc
     4921 agaacacgac tggtttgtcc accacctgaa tcagcccgaa ccgttctgcg gtcctgaaat
     4981 tcacgctgct tctgcgcccc cgttcaacct gcagggacac ctgcgttctg aacatctcca
     5041 gcgaatatgt tgttttcagc aggttgcacg gcgcacaggc ggggacgagg ttttccggac
     5101 agtcggcctc cggtctgaaa acatcaccgg tggatttcag cctgaaaatg cgcttttctg
     5161 cagccttcat gcactgctct gacttgcgga gcaccgcctg aacatggtcg gcatgccatc
     5221 ctttctccgg cagttcacag ccgcagtacg cacaacggcc accgaatttc atacgcagtt
     5281 tttcccgctg tttctttgtc agcgccatca atgcctcctt aacaggaaca gatttacgga
     5341 ttcactaatc ggaccagcgt cctgaagtct gcacgcagtg agcggcgaca gtaccggtga
     5401 taccagtgat gagggccttc aggctgaagc cggaagcaaa gctgaaaatc actgttaaaa
     5461 gtccccttca ccgaccatcc ccatgtcaga cgtggtattg ctgattcagc atcctgccgc
     5521 agtcgctgct ccagaagggc agccattttt accggacagg caaaggcgat ataaggcggc
     5581 agcgttgtaa cagggaaacc atgcccctga caggacgcat atgtacgaaa tccgtgaacg
     5641 ttcatcgcgg ccaccagtgc acggatgtcc gggtcaatcc ggtaatagcc tgcaggtaaa
     5701 ctcattgcga cttatccccg gtgccgtcca gcagtccctg agccacctgc cggcagtctc
     5761 ctgtggcgac cactttctcc agcgcctgtc tgacgcgcag atttccccgg atgatgtcgt
     5821 gcccctggct gcagaacacc accagagccg gcacgctgcg gatgccgtac tgtgaaaaca
     5881 gcgtcgggtc gatctgaaca ccatcggtcg caccgtcttt caccagagac agcactgctt
     5941 cagccgtggc tttcaggtcg ttgttcacca taccccgcag tgtggccgga ataccgtagt
     6001 gccgggtttc gcccagcatc cgtttcagcc cctcttcggg tatggaaaac gacacaaaat
     6061 acacggcacc ctgctgtggt ttatccttct gagcagcctg ctgtttacgg accagttcat
     6121 cgagaaactg gttatcggaa tgctgaaggg gattcgccct gacctgctgt tctgcccatg
     6181 cctgcagctg tgtgtccggc ttttcacgta actgctgact cagtgtttcc tgctgcttca
     6241 ggaactggcg gttttcgggt gtattcacat tttccgaagc atgggcagaa ataaacagtc
     6301 ctgtggtcag ggcaaaaagt acaaatattt tccggctcat atcatctccc ggcatagtga
     6361 tataatttaa ttaccggcgc acttttcttt cagtctctca gacaaagcat ataattcatc
     6421 catttcttcc tgagtaatgc attttctgct gacagtaatc tgcaccgtag ccacggcggt
     6481 atctctgtcc agagtaatat catcaccgta attttttaat gacagatggt ctccctctct
     6541 ccggacctgg tatatttttt ccggtgggta ttcagacaac catttctccc acctgtcttt
     6601 agatttatca ttacagttat attcagacat aatgctgatg aactcccgaa atcttctgtc
     6661 aataaactcc agtcggttca gtatatcctc tgatttcctg atatcttcaa tatctgtaag
     6721 ccgacggcac tcctgcagag gataatatgc cccaccatta ctttcagccc attgtctgac
     6781 cttatcaaaa gcactcacct tttccagcag tgagaaccaa ttctcctcac tgataaaaac
     6841 ccctttttct ttatcactta tctttcgtaa aaatgagaaa aatcccataa cgcaccttta
     6901 cttatattgt acacatgcac acactgttat atttatgata accatcagac tcatttcctg
     6961 cgcttaacgg gcataaactt atctgaagaa agatatgaaa taacaacgcc caccactgta
     7021 agaataaaag acgtcttcag atacagtctg acaagcccga ttttttccag tgtatctgca
     7081 tcatgccagt cattaacaat gtctgagaga atatattcag ggcaaaaata aaaccacacc
     7141 acagagcaca tcaccatagc aaaaggaaac agctttaata cattgctcgt taaccggata
     7201 aacagaagac atatttttct gaagtcatct gcgtgtttat ttcttattac ccacaccttc
     7261 atataatcga tcctcttatt gattaacatg acttacagga acacacagtt acgtttacgc
     7321 cacatcaggt aaccgaagtt tttcttagtg ttgggcggat tttttcccgt ctcccagcgc
     7381 atcacactgc gtccgaacgg atggcactgc ccgctgtccg ggtacatatt caccatctga
     7441 taacgccagc gcgctttggg aagtatgggc gacggatatt cataacagac agccacatcc
     7501 acgccgatgg tgttcattat cattccctga cggtggagct taaacgccat ccgttcactg
     7561 accagcaggg atgactgcag cggactggat tcattgctca cccagccatt aaacggatac
     7621 atactgccct gcgaaccggc acaccagaac aggatatcca gcggcttgtt aaaggcactg
     7681 gcaatggcat ccgccgcaca ggctccctgt gctatcggat ttgcaaaaat gaccgcctcc
     7741 ggattgagga tggtggtcag gctgctgtcc gtccaggtag ggtcaatttc tgacagatag
     7801 gcaatatcca ggtctccgcc ttccaggcac cccaacgacg tgatgatgtt cagccagtac
     7861 gtcagcgggt atttgtacca gtgaacgtga tagaacgccc cgttcacctg tttatcttct
     7921 tttttagccg taccctgtgc cgttttcccg aattccggca gactgaaccc cagattaacc
     7981 atgcatcccg gtgagcgggt gacatccgtc agtgccatcg gctcccagta accaatagcc
     8041 agcccgatac gtttaaaaac agggggaggc gccgggcaga actggagcgg cattgacggg
     8101 tttgccgtat ccggcacctt tccctggctc acttttgtgc tgcccaacga gagagggaag
     8161 atgcagctcc agcagatatc cgtgatcggg ttaacaaacc gcccttcaca ggcagcgtcc
     8221 gctgacgctg cgggaacatg gccagtcagc agcaaaacca cacacaacca catacaccgt
     8281 ttcatttccc tccctccccg accggaataa actccacctt caggaaacga tcgccactga
     8341 cagcactcac ccgtgccggc acctgatcaa tgccgagacg ctggcagatc acgccgttct
     8401 gatcaaagta aacacgactg tccagagcct tctgcatctc cgggatactg ccctgcacca
     8461 ggataatttt gctttccagc gtgggaggcg tctggcgctt catccattca atctgctcag
     8521 gatcatcacc gttgatgaaa tacagtgtct ggttaaaagg cacatactgc agcgggttca
     8581 tcacctcccc ctggcgggcg aagatacgac cttcgttatc ccggatatcc gcagccagtc
     8641 tgaccgacgg gtcaaacagg cggctgctgt atttctccgt ccgaccgata ccgggcacgg
     8701 cagggggccg caggctgttg cggatcaccc gcgccttaaa cgcatccatt ttccggccca
     8761 tctctccgga ctgctccaga gctgtcagac gctgcataat caccgtcagc atgtccggct
     8821 cctttaccgg ccacaaatcc ccccaggtgc caagatcagc tgcagccaca ctctgccccc
     8881 ataccagcag ggcaatcagc ccccggcatc tcatggttct gccctcattc gccgggcaat
     8941 atcctgctgg atttcacggg tgatatccgg tgtcccctgt accacggcag gcgacaccag
     9001 aatgacagcg tggtgcttct gctgccacgt ctgcagactg gcctccagtg ccgcgttaaa
     9061 acggtcagcc agcgccttcg actgcacttc tgacagtttt ttctggctgg cgctgtcaaa
     9121 aaacgcatcc actgtctgtt tcatgttgaa ggcgacggtt accggcgtgt tcagtctgac
     9181 aatggcgtaa ctgacggctg cgttcagtaa caccattgcc aggcatcccg gcaccgtcca
     9241 ccaccagtgg ctgcgttgac gggctgtaac atcagtcgtt ttttgcgttg tggtcatgca
     9301 acactcctgt atttctcatg ttcttccagc caggcttcca gcgaagccat ctccggccct
     9361 gacttcttcc acgccagctg ccacaccgcc tcatgaatac tcagcccctc cttacgtttc
     9421 tgctgcacaa actcaaaatc cgggccgtca gagctgtaca tggcacggct taacgggtcc
     9481 acaaacagac ggtgccagga ggaatggttt tccacctgca gcaggaagga actgaaccac
     9541 tggtctttgg ccgcgccaaa cttgccaatc atgtcacgct gcagtggcag gaactggtcc
     9601 ggatacagct ggttgtattt cgcgaactcc ttcgcactct gtttgaggat aattttgtag
     9661 gaggagttac cccatgccgc acgggcagca ctggaggcct tgtcagagtc aaagtcgacg
     9721 atgttctgtg tgatggtgat ataggcaccg gtatgacgac gggcggtacg gtaacctttc
     9781 tcaataaact caccgacctt gtgatttttg aagtccagca gacgccagcc ttcatcaata
     9841 acgttcagtt tcttgaggtt acgcggcgtg cggtacatcc ggttctcgat gtaaatgata
     9901 aggctgaaca tcaccgcaac cagcagggac ggacggtctt ccagtccacc cagctccagc
     9961 accaccattt tcgcatcatc ccgcagggac ggttcatcag agttaaaata ctggccgtaa
    10021 gtaccgttgg cagtgtactg gtccagcagc actatcattt catccagacg gctgcggatg
    10081 gtcggggact cggcatactg gtcgttatcc cgggcgtttt tcaggaaatc caccacatca
    10141 tcaatacgcg cccggttctg tctggtaagc caggaggccc tgaccgcctg cagcagcaga
    10201 ccttcatgca cttcatccag gttaccgttg gggctggcca tcaccgacaa ctggtcacgg
    10261 acacgctccg ctgactggtc aatatcggtg atgttcgcaa acggattaaa gcgcagggtt
    10321 tcaccgtcca gatatacgcc ccccatgttc tcacacaggg acttgtagcc atcccccatg
    10381 tcgaacacca cggcaaagcc cccggagtcc agcacgctgc ggataagtgg ctgtatcagc
    10441 ccggttttac cggccccgga ggtgccacag accgccatgt tgtagttggt gttattcatc
    10501 ccccggaaga aaatatcgat aaacgccagc tggttacggt aggtgggtgc cagcagacct
    10561 gccggtgtca gggggttatc cgccactaac ggcatcaggt tggccacatt aaagctctct
    10621 gcgcgctgaa ccacaccggc ctctttcagc tgtttaaaca gccctttccc ggccataaag
    10681 ggcaggcagg tcagaaaatt gcgcatgtgg ttaaagcgcg gtgaaatcag ctcaaaaccg
    10741 tttttacgga agctgttcag gatgtcctgc tccacttcca gcgccgtttc attgttgtcc
    10801 ttgcagaacg ctgtgatgtt gaggaagtag gacaccacgg aggactgacc tgagcccagc
    10861 cgctggcgca gttcccccca ctccttcgct tctttctcca cggacggaaa ccatttggca
    10921 taggaggtct tcgacttctt ctccaggtcc atgtacttca ggttggcttc gctgtgggtt
    10981 ttcacctggt cttccaccac cagggccagc gtcaggatga acgggcagga aatggacatt
    11041 tccgggttca gcaggttgct gtagttgtcg gccatgttcc acaggaaggc gatttccggg
    11101 ttacgggcca gatggaaatt caggatgcgg gccgtgctgt tcctgccgtt ctcacgcagg
    11161 cccagcgtca ggtaatcagc ccggactttc agatcaaaac tgtcctccac acactgataa
    11221 ttcaaatcag aatacgggtc cagctgacgt cttttcgggt acagggagtc cgggttatgg
    11281 ttaatcatct ccccgacaat atcgatgaaa gcctgtgcat ccaccgtctg cgtggtgata
    11341 tatgctccct gcagagacgc ccggatgatt ttcaccaggt tttccatttc cagaatgtcg
    11401 gcccggcttt ttttcttcga gggagaacag tacgagataa acacccggta atggcgcagg
    11461 gtcaggggca gattcattcc ctccggcagc ggaaactgtg tttctgcggc tttcatataa
    11521 taggcacggg tgattgcgtt aaaccgctca gcctgctcac cggaccagga gaactcccgc
    11581 aggccgtatt caatcctgtc accaaccagc tgactggaca tcagatggat acacaacgga
    11641 ataccgcgcg gcagtttggt acgcagcatg tggtcgagcg cctccacaat ggactcattc
    11701 gccccattaa tgggaatggc ttccagcata aagcccagcg tggtgtcatt catgaacaga
    11761 ccagattcct ggttgtaatc ccggtacggc agcagacggc tgaactgcgg gaagctcatt
    11821 tcacccagta tttcattggc ctttgcggat tcatcaggca gtttcagtgc ggtgacaagg
    11881 gagttcaccg cctgagtgac ggcctcaagt gggttattac tcatacgagt attccttagt
    11941 acgtttaaaa aaggaaaata tcagtctgtg gccgtggata tatcacgcag tgccattctt
    12001 aaaaccacag cacaaaataa gaaatggagg cttttaacag gaaaccagga ttaacgttcc
    12061 tttactgaat gagcatcatt taatgcctga agttgttttt cgcgtatttt attgcgctca
    12121 ttttctctta cccgtcgcgc ctctgccatt tcatttcttt ttctgcattc tgatgcgatt
    12181 ttatttgcct taccaatggt taaaggttca gactgataat taagtgtctc ctgaaatgct
    12241 tctgctgcac agggagtttc ctgtaaaatc tctgtcagtt gttctggtgt tgcaggttgt
    12301 tgcgcaaggt aaacgatgta aaaaggaata catacaatca tagccataac accaacaaat
    12361 acaaaagaca gaaaatattt taaatatatc agcttcattt aatcttagcc ttatatagtc
    12421 cgataagtac gctaacaatc aatgtgttac ctctgttgac tcgcagactg aatttttcag
    12481 ggataacaaa catagccagt gcgttaacaa cacagataac cacaagaaag aggcacaggc
    12541 atataaacca gtcaatatta tttccttcga agcagtaagc attgcccacc accattcagt
    12601 gtttccatca cattgttcaa aggttcattt gtttgaactt caatacattc tcctgtattt
    12661 ccaaagcccc tgcctccatg cgcttcgatt gtccgaagaa cctgcacgca tacatccgta
    12721 tttggcatgg gtgtaacatt ccagcttccg ccaccgttaa tattttttat cagcacaaga
    12781 aaaataaagg tatgcattct ttattacctc cagtgaaata ttaatcactg aatattcctt
    12841 cagttaactt atgaggttat aaaacacagc agattttcat tcggatttac tctcttcaga
    12901 aaactgccgg atggtatcct gttcacgctg acgagtggca gactcctgca ttgcatccat
    12961 cttttcccac cagagactcc tgatttccct cttgtccaga ccggtcagtt ttttaccgga
    13021 cagcaggcgg ttaagcagtt cctggtgcat atccggacag tcttcagtca gactcataag
    13081 ccagagcaat tcctgacgac tgagtaccct gcgtgattcc tccggtacgc tgtaataatt
    13141 aaaaatacga ctgaataccc cacaacagca ggcaacggat aggcacaggg atttcagata
    13201 caggccagaa acacagaatg tggcaacacc acaaagaata gctataacta aaaagccctt
    13261 acccagtgcc atacacaaag aaccctgttt tctgaatatc cgctcatgct catccagaat
    13321 aaaatctatt ttttcatttt ctgatttatg catagtgttt cctctgcctt tcctgatacg
    13381 cctgacattc aacacataac gtcacaccgg gaaatatttt ccgccgggct tccggaatag
    13441 gatttccgca tgcttcacag agataaacag gaataccatg agcatttatt ttctggcgta
    13501 tccggtttat tcctgtcatg gtcagttgtt ctgtcactga atatgcttca tcggcttcat
    13561 cactcaagga atattcctcc cgacatgttc tgacacgcta ccgcccatcc cttttcacgc
    13621 acagggcact cgttttacag gcagattttg aatacagaaa gcccgccggc attgccggct
    13681 gatatacaac ttcagggcct gttaattaat acgtggtttt ccccacgcag acggtttaat
    13741 aacaaaaaac acgctggatg gctgatgata aacatcctga ttatcaatat acggcgctat
    13801 ccataatgcc gccgtctgtt cacccgttct tagcggacgt gggggtgtta ccggcacaac
    13861 tgaactgacc ggaacaaccg ttttcacttc ccgggcagcg gtaaacagcg ggcgtggtgc
    13921 ctgcagtttc tgctccggaa gtgctgtcac agcaggcctg cttcgggacg gcgttgtcgc
    13981 cgcaacggcc tgcaccgacg tagtcctgaa gttcccgtca gccaggcgcg gcagtgatgc
    14041 cgcaaccggc tttgcatctg caggctgttc catctttttg gctttttcgt tggcctgttc
    14101 cattgtcatg caggtatctg acgtggtggc atcacactca aaatcagagt tcgttccggc
    14161 gcagccggaa agtaataagg ttcccagcag aggaacaaat aaagaaatct ttttcattgc
    14221 ccttctcctt ttttattatt ccccacaaca gcagaaatta actctgcagt cagatttgct
    14281 ttccccgcac aggtttgtcc gacaaccata atatgccccg gagaagaaaa taaactcatc
    14341 gacatcccct tcttcatcaa accgacagcg gctatattaa aattgtgcag cagtgattga
    14401 ggactgcttt taataatatt aagattacgc atgttcattt ttatctccgt tatttctgat
    14461 tgctctgtca tcagggaaat aaacagggca tttccttatt ttttccggca gggaagcgtc
    14521 ttccgtataa actcccttca gacgttcatc tgcggctgcc acccggacac atttcatttt
    14581 tccgggccac atcggatatt tcccggtgcc tgccgcaaac gacatataca tcagtgccag
    14641 tgttgcccag aatgcggtgg tcagttgcat cagtgagcgg tcagtctccc tgacataatc
    14701 tgcagcatca gcagcacggt aacggcagat tttctctgct ccccccggaa ggtcatgcag
    14761 agaaggcaga cattctctta caggaaactg cccggcaggg gctgtttgtc ttacgaagtc
    14821 aatttcctga accagtaatt ttcctggtgt ggtgttgtcc ttccataaaa caaataacgc
    14881 catcagggcc attgtcgcca gcggccagat aaccacaaac ctgacgaccc acagcagcca
    14941 gcgaacgaca aacgccacac ggaatatcag tgcatcaccc accatttccg ccctccttac
    15001 cggcatcccc gccctgctga ttcctgtctc cggacataaa ctgctgcgga ttcaggttgc
    15061 ccagctgttt cagcatctgg tcggtgttaa aaccgttcag gtggctttct gccgtgggcg
    15121 gattcggcac cgggctttca gcgttcccct cctccgcctg gctctgtgac tgctccagcg
    15181 ccatctcctc cacggttttc agctggaagc catcctggaa caccaccgtc acttcattgc
    15241 ccgcaccaat cggtatcacc gggtgatact gctcagcgcg tttgatgtag tagtcactga
    15301 gtgtctgtgc cgcttttgat gcaccgccac cgatacccat tttcaggaca tcgccggctc
    15361 cgtaagcggc agtggctccc agcccgacag caggctgtga agctcgctcc attccctgac
    15421 cgataccgtc aacaaatccc gcgccccatg cccagccgag gattttgccg ttacgcatca
    15481 ccacttcgcc cttgataccg tttttacccc ggaagctgac atgcccctta atcggcatat
    15541 caatagtttt gccgtccttc aggcagctga tattgcgggt gcgtacaatg gcacgctcac
    15601 tggacacatc cccccaggct tccagaccca caaaacatcc cgtggcgtca tacgttttgc
    15661 tgttcggcat ttccaccagg ccggtgatac gcagctgcat cggcacagtg gattcattac
    15721 cggtgacaga cgcattggca tctgccccct caatcaggac ggattttgca aagctcccgg
    15781 acgggatata cggcagggag gggcccttct ttctgtcagc gtcatagctg aacgttttac
    15841 gctgaatacg gttcggtacc ggcacagact ggtatgtcac ctgcggaggc ggtgtcgtgc
    15901 cgttccccgg ataaaacgcc gtcggcggtg gaacagcacc ggtgccctgc ggagggaaag
    15961 atacgggtgt gtttcctggc tgtggttcgc cttccggccc gggcggtgag gctggcgtct
    16021 gcggtacagg ctcaccggtc gcggtgacgg gatttgcacc cagggctttt acctgctctg
    16081 ccagggcggc attgtcctgt cccagctttt caatacgacg ctggtcatca ccacgctgtt
    16141 tgttcaggac atccagttca cgacggattt cctcatactg cttctgcatc tgcgctgccg
    16201 tcacctgcat ctctgtggtg gcatgctgac gcaccttgtc atcaaaggtc gtatccacca
    16261 caccggtcat atccggcaca ggctcctgtt cagccacggt ttcaccgtta ccggacatgt
    16321 ccacatcaga cagatacagt gccccaccaa tcgcggaggc tgtaccgaca accacaatcc
    16381 ccagccacag gtactgcttg cgtttcacaa tggtattgat actggccatc agttgccctc
    16441 cccgtcacgg atgacataca catccatgcg cgcacctgcc agtaactgac tggcaggctg
    16501 gctgaacatc acggcacggg ttcccgactg ccagaaatca ctttcccgga tattcagggc
    16561 agacagcgtt ttattttcca cgacaaagcg gaccatcttc aggtgattcc ccgtccatac
    16621 ggaatctgcc actgaagaca gccccgccgg ggcctgcagg gtttcctttg tcacaggaac
    16681 ctgataccag ccggcgggta attttccgcc acggacagcc tggctgatgg tcacaagcag
    16741 ggattcgtaa ggcgtggacg tttcccacgc gccggcttct tctccggttc cgcgcaggtc
    16801 actgaccagc tgaatggtac gccctgcgcc ttcacgggga acggcctgaa tggaaaggtt
    16861 aagaccacgt tctgtttcca gaatgaacgt aaaaggcttt ttgttgacgg tggccaccac
    16921 cacaccgccg gaggcggtct gctcattatt ggtcagggca ccatccagac tgttcacggc
    16981 gataatccgg tcaccaggaa cggtaaacag attcgggctg gtattgctga tggcaatact
    17041 ggcctgacca ccgttcacca ttggcaccac ggtaggggcc agcgtgccgt ttgcggccat
    17101 cacgctgcag gaaaaaaaca ggccgccgaa tattattgct gtattatttt ttctcatcgt
    17161 ctgtttcccc gaaattatcc agccaggtca ccccgttttc ccgcttcaga ataaggatgt
    17221 aatgtttgat atcagtaaaa ggttcggagt taccaatcca tgttttccga attccacgga
    17281 tttccacacg accatattgt ggccagacac ggacactggt ctggaaaaaa gcagaattca
    17341 cgttatcttt tttaatacgc ttcgcttctt cagccagaat aactttcatc tgattctgtg
    17401 cgcccggacg gatatactgc agaagcgcct gatgtgaggc atcgacgatt tccggtgaaa
    17461 cattcagacg aagggcaata aatgccagtg ccatctgctg taaataagag gcatcggcac
    17521 tgttctgtga cacggcaaag ggtacattaa atcccatcgg ggtcacggca gtgcgttgct
    17581 cattctgaag acggtagtta tttatcccct gaatgacgtt aacggcgagg ctgaggacaa
    17641 taagcaccga cataaatata aaggcgatgg ccattacacg actggtactt aaacgggcac
    17701 cgtgttccat agaataatcc tggtatcagt tctatttaat ccactgccgg aaacacgaat
    17761 cgggaacatt atgaaaaata ccgcgcaaca ggactgttgg catataccag taaatcaggt
    17821 cacgtaacca gaagctgccc cgcccttttt tcagtttttt aatcccgaaa taaaccagaa
    17881 ccgctgcacc aataccaaac agatattttg atgtcatcat tccccagcca atacaaattc
    17941 cggcaggaat aagttcatcc agcggcaggc caaaccagcg gctctggttg gtcagtgttt
    18001 ccgggaaacg atattttttg agtttattct cgtctcccga catgacgtta tttcctgtca
    18061 gagaccgacg actgccatac ccacggtaat aaacacggag ataatggcaa aaccggcgag
    18121 gaacttaacg tttttggtca tcatgtacat gatggccccg acgagaactt ccgccagaat
    18181 cacccatttc accgcactgg agtctttacc gaaggtggcc ttgacggtgg cgttaccccc
    18241 ggccatcagg tcctgacctg ccccctgggc ggccagtgcc agttgcggga agaacatcat
    18301 cagaacagca gccgggatca ctgcacgaac caggcgaagc atattcagac gcgtgaattt
    18361 aggaaaaaac gacttctttt tgacgggcgc agaagcaccc tgaacactca aaatagcatt
    18421 cataagttac ctctattgtg gatagattaa taaacttaaa gatctttaat tatcgaatca
    18481 gtttcccctg ttccctcctt tattgcatcc agattataaa agtcaggaaa acggctcaga
    18541 tgatcacgta acctgactag cacctcatta gtttttgttc tcccgctacg ctcccgggca
    18601 cctaagagct tgtgattagt agcatcgtcc aataccagta aaactttatt gcccggagca
    18661 ggccttatta tatttctgct caagtctccc cctcagccat ttagataatt aaatataatt
    18721 atatgaatcc ataaaaatag aaacaaaaat agagtaaatt acattaacaa aacaaggtga
    18781 cataatgtca ccttgttttt aacaaaa
//
LOCUS       DAZUCE010000088        18690 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZUCE010000088 DAZUCE010000000
VERSION     DAZUCE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.088"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            247..939
                     /gene="yieP"
                     /locus_tag="ACHY03_002569"
     CDS             247..939
                     /gene="yieP"
                     /locus_tag="ACHY03_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFS3905782.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VINHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            962..2389
                     /gene="mdtD"
                     /locus_tag="ACHY03_002570"
     CDS             962..2389
                     /gene="mdtD"
                     /locus_tag="ACHY03_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFS3905783.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2355..3347)
                     /gene="rbsR"
                     /locus_tag="ACHY03_002571"
     CDS             complement(2355..3347)
                     /gene="rbsR"
                     /locus_tag="ACHY03_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFS3905784.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3351..4280)
                     /gene="rbsK"
                     /locus_tag="ACHY03_002572"
     CDS             complement(3351..4280)
                     /gene="rbsK"
                     /locus_tag="ACHY03_002572"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFS3905785.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4406..5296)
                     /gene="rbsB"
                     /locus_tag="ACHY03_002573"
     CDS             complement(4406..5296)
                     /gene="rbsB"
                     /locus_tag="ACHY03_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFS3905786.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5321..6286)
                     /gene="rbsC"
                     /locus_tag="ACHY03_002574"
     CDS             complement(5321..6286)
                     /gene="rbsC"
                     /locus_tag="ACHY03_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFS3905787.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGFVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6291..7796)
                     /gene="rbsA"
                     /locus_tag="ACHY03_002575"
     CDS             complement(6291..7796)
                     /gene="rbsA"
                     /locus_tag="ACHY03_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFS3905788.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7804..8223)
                     /gene="rbsD"
                     /locus_tag="ACHY03_002576"
     CDS             complement(7804..8223)
                     /gene="rbsD"
                     /locus_tag="ACHY03_002576"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFS3905789.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKHHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8390..10258)
                     /gene="kup"
                     /locus_tag="ACHY03_002577"
     CDS             complement(8390..10258)
                     /gene="kup"
                     /locus_tag="ACHY03_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFS3905790.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10481..11977
                     /gene="ravA"
                     /locus_tag="ACHY03_002578"
     CDS             10481..11977
                     /gene="ravA"
                     /locus_tag="ACHY03_002578"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFS3905791.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11971..13422
                     /gene="viaA"
                     /locus_tag="ACHY03_002579"
     CDS             11971..13422
                     /gene="viaA"
                     /locus_tag="ACHY03_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFS3905792.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRNRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLNQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13427..14419)
                     /gene="asnA"
                     /locus_tag="ACHY03_002580"
     CDS             complement(13427..14419)
                     /gene="asnA"
                     /locus_tag="ACHY03_002580"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFS3905793.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14572..15030
                     /gene="asnC"
                     /locus_tag="ACHY03_002581"
     CDS             14572..15030
                     /gene="asnC"
                     /locus_tag="ACHY03_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFS3905794.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15120..15563
                     /gene="mioC"
                     /locus_tag="ACHY03_002582"
     CDS             15120..15563
                     /gene="mioC"
                     /locus_tag="ACHY03_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFS3905795.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15942..17831
                     /gene="mnmG"
                     /locus_tag="ACHY03_002583"
     CDS             15942..17831
                     /gene="mnmG"
                     /locus_tag="ACHY03_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFS3905796.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17895..18518
                     /gene="rsmG"
                     /locus_tag="ACHY03_002584"
     CDS             17895..18518
                     /gene="rsmG"
                     /locus_tag="ACHY03_002584"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFS3905797.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4692 a         4864 c         4619 g         4515 t
ORIGIN      
        1 gaattctcag aagaaccccg ctgacccggc ggcgtgtttg ccgttgttcc gtgtcagtgg
       61 tggcgcatta tagggagtta ttccggcctg acaagaggaa atttaaaata attttctgac
      121 cgcgcatttt ttcagcaaaa gccccgttaa tgtgccataa aacaagcaat ttgccgtttt
      181 tgcaaccgta atcacacttc ctggtggcat actaaggagg aataaaaaga aaaaggaaga
      241 ttagtaatgc ctttaagcgc acaacagtta gctgcacaga aaaacctttc gtatgttctg
      301 gctgagaagc tggcgcaacg gatcttaaaa ggtgaatatg aacccggcac cattttgcct
      361 ggtgagattg agctgggcga gcaatttgga gtgagtcgta cagcggtacg cgaagcagtc
      421 aaaacgttaa cggcaaaagg gatggtttta ccgcgaccgc gaattggtac ccgggtcatg
      481 ccacaatcga actggaattt tcttgatcag gaattgctta cctggtggat gacagaagag
      541 aactttcatc aggtcatcaa tcactttctg gttatgcgta tctgtctgga gccacaagct
      601 tgcctactgg cagcaacggt tggcaccgcg gaacagaaag cgcatctcaa tacgttaatg
      661 gccgaaatgg cggcattaaa agagaacttt cgccgtgaac gctggatcga agtcgatatg
      721 gcctggcatg agcatatcta tgaaatgagc gccaatccgt ttttgacctc attcgcctcg
      781 ctattccatt cggtttatca cacttacttc acgtcaatta ccagcgacac agtgataaag
      841 ctggatctgc atcaggcgat tgtcgatgcg attatccaaa gcgatggcga cgcggcattt
      901 aaagcttgtc aggcgctgct acgctcacct gataagtgat aacccgataa caggacattg
      961 aatgagcgat aaaaagaagc gcagtatggc gggtttgccg tggatcgcgg cgatggcctt
     1021 cttcatgcag gcacttgatg ccactattct gaataccgcc ttacccgcaa tcgctcatag
     1081 ccttaatcgt tctcctctcg cgatgcaatc agccatcatc agttatacgc tgacggtggc
     1141 gatgcttatt ccggtaagcg gatggctagc cgatcgcttc ggtacgcgtc gcatttttac
     1201 ccttgccgtg agtctgttca cattgggttc tctggcctgc gcactttcta attcgctacc
     1261 acagctggtt gtcttccggg ttattcaggg gataggcggc gcaatgatga tgcctgttgc
     1321 tcggctggcc ttactgcgcg cttatcctcg taatgaactt cttccagtat tgaattttgt
     1381 cgccatgccg ggtctggtgg ggccaatttt aggccccgtt cttggcggcg tgctggtcac
     1441 ctgggcaacc tggcactgga tatttttaat caatatcccc ataggtattg cgggccttct
     1501 ttacgcgcgc aaacatatgc ccaatttcac caccgcacga cgcagattcg atatcactgg
     1561 ctttttgctg tttggcctca gccttgttct cttttcaagc ggaatagagc tattcgggga
     1621 aaagattgtc gccagctgga ttgccttgac ggtaattgtc accagcatcg ggttactgct
     1681 tctctatatt ctccatgcac gacgcacgcc aaacccatta atttcattag atttatttaa
     1741 aacccgcact ttctcgatcg gtatcgtagg caatattgca acccgtctgg ggaccggctg
     1801 tgtaccgttc cttatgccat tgatgttaca ggtaggattt ggttatcagg cgtttattgc
     1861 cggctgtatg atggcaccga cagcgttagg ttccattatt gcaaaatcga tggttaccca
     1921 agtcttacgt cgtctgggct atcgccatac gttagtgggg atcacggtga ttattgggct
     1981 aatgatcgct cagttctctt tgcaatcacc ggcaatggcg atatggatgc tgatcttgcc
     2041 gttgtttata ttagggatgg ctatgtcgac gcagtttacc gcgatgaata ccatcacact
     2101 tgccgatctg accgatgaca acgccagcag cggtaacagt gttctggcgg tcacgcagca
     2161 actgtcgatt agtttaggcg ttgctgtaag tgcggccgtc cttcgcgttt atgaagggat
     2221 ggaaggcaca acgactgtcg aacaattcca ctatacgttt atcacgatgg gcattattac
     2281 tgttgcttca gcagcaatgt tcatgcttct gaaaacaacc gatggtaata atttgatcaa
     2341 aagacagcgt aaatctaagc cgaaccgcgt tccatcagaa tcggagtaag ttgtaatcgt
     2401 tgttgctgaa gagttggctg ggttatccga tggatgagta catcaatcgc cagctccccc
     2461 agttcatctt tcggttggtg gatagtggtt aatggtggcg tcataaagct tgccagttcg
     2521 atatcgtcat agccaatcac cgcgatatcc tgcggaacct gtaactctgc ctgatataac
     2581 gcctggtaaa cgccaacagc catagcgtca tttccggtaa agacggcctg aggacgcagc
     2641 ggatgtgata gcagttggcg catagcgtca aacccaccgt taaattcaaa atcaccagtg
     2701 acttcatagc catcaggaat gttgagaccc gcacgtttca tcgccgcccg ataaccttcc
     2761 aaccgcaggc gcgccggagt tttatccagc gggccggtaa tacaggcgat acgggtatga
     2821 cctttatcga tcagatattg cgttgctaag tctccgccca gcaacgagtt atcctgaata
     2881 agatcgctgt cgccatcgaa cggagcccag tccatcatca cggtaggcac tgtcggataa
     2941 cgttgcatga tttcacgcga aggctgatgc gtttcggtgc acagtaacag caagccatca
     3001 acgcgttttt gcatcagcgt ttccagattg cgattcatcc gctgttcatc gccttcggta
     3061 ttgcaaagga caagactata accgcgttcg aagcagctgc gttcaacgcc gcgcaccagt
     3121 tctgaataga aaggattagt actggcagtg atcaacatgc caatggtatg tgtttgattg
     3181 agtttgaggc tacgcgccag agctgatggc gcgtaattga gttctttaat cgccgcttca
     3241 actttggcgg taatcgcttc actgacgaag cgatctttat tgataacgtg agaaactgtt
     3301 gaggtagaaa cgcccgccag gcgggcaaca tctttcattg tagccaagcg tcacctctgc
     3361 ctgtctaaaa atgcgtcgat ctcttcacgc cacggtacgg aaggttgtgc gcctttacgt
     3421 gttacggcaa tcgcagcggc ggcatgggca aaacgaatcg cctctggcaa tggtttttct
     3481 tccagcaacg ccgtgattaa cgcgccgtta aaggtatctc cggcagcaat ggtatcgaca
     3541 gcctgcaccc ggaatccagg aacgcgctga ccttcaccat tcacgctagc ccatacacca
     3601 cgacttccta aagtaatcag tacagtacgg ataccttttt catgcagtac ctgcgccgcc
     3661 ttcgctgcat cttcatcatt ttcaacacga ataccggtga gcttttctgc ttccgtttcg
     3721 tttggcgtaa taatgtccac cagcgccagc agttcgtcag gaagttcgcg agccggagcc
     3781 gggttaagcg caacgatagt cttattttga tgggcgattt tcgccgctgc catcacactt
     3841 tcgagtggtg attccagctg cattaataat gctgacgcgt tggcaatacg ctcacgttgc
     3901 gcttccacca gcgccgggga aagggcagca ttagcgccgg catgaatacc aatgacattc
     3961 tcaccttcgc cattaacaaa aatcagcgcc acacctgttg attcgccttt gatcacgctg
     4021 accggagtaa tatcgatgtt atcagtggcg agctgctggc gaacgctctc accaatgctg
     4081 tcatcgccag tacaggcaat aaacgcgata ttcgcaccgc tacgcccagc agccacagcc
     4141 tgattcgcgc ctttgccgcc aaatgcaacc tgatagtggt taccggttac ggtttcgcct
     4201 ggagtaggaa aagattgaag attaagaatg tggtcagcat taatgctgcc aagaacaacg
     4261 aggctgcctg cgttttgcat attcgggatg tccataaaat gcgccaccgt gttagggtgg
     4321 cgcgtgccct gcttttcttt atattacacg ttcatctgtc gatgacgtat ttatgtcacc
     4381 atcaggtcat acaacctgat taaaactact gcttaacaac cagtttcaga tcaaccggat
     4441 acttagcctg aactttctcg cctttcagca ctttatctgc ggtttcaacg cctttcgcgc
     4501 caatctgatc gggtagctga gcgatagtcg ctgctagttt gccatcattc accgcttttt
     4561 cgccatccgg tgtaccgtca aatccgacga ccatcacatc cgatttaccg gcagtttgca
     4621 gtgcgcgcag cgcgcccagc gccatttcat cattctgcgc aaatacagcc tgaacatccg
     4681 gatgagcggt caacaggttc tgcattacgt tcaaaccttt agtgcgatca aaatctgctg
     4741 gctggctggc aagaacatta aacttgtgag cagcaacggc ctgctggaag ccttcgccac
     4801 gttcacgggc tgcggatgta ccagcaatgc cttgcagctc gataactttg gcaccttcac
     4861 ccgctttctt cgcgatgtaa tcaccggcga ttttgccgcc cagtacgtta tcagatgcaa
     4921 tgtggctcac cacttcacct ttcgttgcct ggcggtcaag agtgataacc gggatgttcg
     4981 cctggttagc catcttcaca gcattaccca ctgcgtcgga gtcggtcggg ttaatcagca
     5041 gaatttttgt gccgcgaacg gttaagtcct gcacgttcgc cagctctttc gccgggttgt
     5101 tctgggagtc cagcaccacc aggttatagc caagtttatc cgcctctttc tgcgcgccat
     5161 ctttcagcga tacaaagaac gggttgttaa gcgtggagac caccagcgcg atggtgtctt
     5221 ttgccatcgc attcgcactg acggtggcgc ttagcgcaac agcggaaacc agggtagcca
     5281 gttttttcat gttcatattc aagatgtcct gtagtcgtta ttactgcttt ttgttgtcta
     5341 ccagcaccgc cagcaaaatc accaccgctt tgacgatcat ctggtaatag gaggaaacac
     5401 ctaacaaatt caatccatta ttaaggaagc caagaattaa tgcgccgatc aacgtcccaa
     5461 caatgcgacc ttttccgccc gccagactcg taccgcccag aaccaccgca gcaatagcat
     5521 ccagctcata gccagtcccc gccgtgggtt gtgcggagga gagacgcgcc acttcaatga
     5581 tcccggccag cgatgccagc agaccacaaa gagaatagac gatgattttg attttattga
     5641 cgttgatacc agaaagacgc gttgccgctt cgttgccgcc cagcgcgtag atgtaacgcc
     5701 ccagacgcgt gtgatgcagc atgtaccagg ccgcgaggaa gacaaacccc atgatccaga
     5761 ctggcgtcgg tacgcccagc ggacgaccaa taccaaacca gccaaacaga tcggcgttct
     5821 cagtaaagcc ggtattcact gggctaccgt tggtataaac catggtcacg ccgcgcagta
     5881 aaagcatcat aaccagcgta gcgataaacg cctggacgcg acctttcgct acaatcactc
     5941 cggttaccgc accaattgcg gcacctaacg cgagagcggc agcgacagcc accagcgcat
     6001 tgacttcaat gccgacgata gatgcagcaa ctgcgccggt tagcgccaac agagaaccta
     6061 ccgacaagtc tatgcccgac gtcaggatca ccagcgtcat cccgaccgcc ataatggcgt
     6121 tcaccgaagt ttgctggaga atattgaata agttattgat ggtgaaaaag ttcggactta
     6181 acgtcgagac aatcgcgatc agcaccagca gagcgataag cgatttctgc tccatcagcc
     6241 acgctttcgt gaaataacgg cgaccagaga cagtctgggt tgtcattttt ttactcctga
     6301 ttcacgcgat taagcttgcc cacagcggca gccattaaca cttcctgggt ggcctgctca
     6361 cgagtaaatt ccccgctgag atgcccttca tgcatgacga tgatgcgatc gctcatgcct
     6421 aatacttctg gcatctccga tgacaccaga atgatgctca agccatcggc tttgaactgg
     6481 ttaatcagtt gatagatctc ttttttcgcg ccgacatcta cgccacgggt aggttcatca
     6541 aggatcaaca ctttggggcg tgtcatcaga ccgcgggcaa tcgccacttt ttgctgattg
     6601 ccaccggaaa gcagaccaat tgcctgttcc atcgacggag ttttcacatt aaacagacga
     6661 atgaaatcac tcacagcctg ctgttcatcg gcatgcttca aactgccgcc agcgcggctg
     6721 aagtagcgca gcgctgtcag cgacatgttc tcttttactg acatgcccaa cactaaaccg
     6781 tcacgtttac ggtcttcgga gatatacaca atgccgtttg ccaggccatc ctgcggtgaa
     6841 cgggtaacga cttcatgccc atccagggtg acgtaaccgc tggtgcgcgg tagtgcgccg
     6901 tagagcactt tcatcagttc ggtacgacct gcgcccatca aaccagagac gccaagaatt
     6961 tcgcctttgc gtaaagtaaa agagacatcg ttaacgccag gtccgcagag attatcgact
     7021 ttcaggcgga tatctcccgg cgctttgtcc aggtgcggat attgatcttc cagcttgcga
     7081 cccaccatca tctcaatcag cgaatcttcg gtcagtgatg ccacttcgcg ctcagcaata
     7141 aattgcccat cacgaaaaac ggtaacgtca tcgcaaatct cgaagatttc tttcatgcgg
     7201 tgggagatat agacaatacc gcggccttgc gatttcagct cgcggatgac gcggaacagg
     7261 gattcggttt cggtatcggt cagcgcatcg gtcggttcat ccataatgat gactttcgac
     7321 tcaaagctca gcactttggc gatttcaacc atttgctggt caccgatgga aagatcgccc
     7381 accagcttgt cgcttttaaa gcgcaggtta agtttagcca gcaatttatc cgcttcggca
     7441 tacatggttt tccagtcaat tttgccaaag cgattaacaa actcacgacc gaggaaaatg
     7501 ttttcggcaa tggttaactg cgggatcagg ttcagttcct gatggataat cccaatcccg
     7561 gcttcctggg aagatttcgg cccggtaaat gtcgtttctt tccccagcca taaaagcgtg
     7621 ccggcatcgc gagtatagat accagtaagc actttcatca tggtggattt acccgcgccg
     7681 ttttcgccca ccagcgccat cacgcggccc ggatagacat ttaacgcagc gcccgagagg
     7741 gcttttacgc ccgggaaggc tttatcgatg cctttaagct gaagtaatgc ttccatgacg
     7801 gcctcagaac gtcacgccag cacagagaat gatattcgca tacggagaac attctccgct
     7861 gcgaattacc gcctgacttt ctgcggtttg ttgtttgaat tgttcatgcg tggtgtaacg
     7921 aatttcaatg gtatttccct ggtgtttttg cagctgctca aggtgagtga gcaacgtttc
     7981 gtggagttgc ggattatggt gtttgatctc ttccgcgata atggcagcct cgacctgcat
     8041 ttcatttgtg acgacgccca gcacctgcat aaaagaaggt acaccctggg ttaatgccat
     8101 atcgatacgc gttgtacttt tggggatggg taaaccagca tcacacacca ccagcgtatc
     8161 ggtatgtccc agacgggaga tcaccgatga aatatcagaa ttaagaacgg tgcctttttt
     8221 catttttttc tccatcagcg aaacgtttcg ctgacccgca gtttaatcct gactgagttg
     8281 ggaaaaccat cgtgacgtta cggaaatgtg atcaacctcg aaacgtttta catggtgatt
     8341 aaccatgaaa acaaaaacgc ccccttatga agaaaggagg cgtctggcgt tagatttcga
     8401 cctgagtacc cagttcgata accctgtttg gcgggatttc aaattgatct ggtgcacgca
     8461 gcgcattacg ttgcagcagc aagtacagct tgccgcgcag acgcaaatac cacgggcgtt
     8521 tgccgaggat caacgactca tgcgacataa agaaggaggt ttccatcatc cggcaactta
     8581 atccttccag accgcagcgg tggaaaactt cttccacgtt tggcgtttct cgccaaccat
     8641 aacttgccac cacgcgccag aaagtgggcg acagttgttc aatctgtacc cgacggacgt
     8701 tatggacata tggcgcgtct tcggtgcgca gagttaacag aatcacccgc tcatgcaata
     8761 ccttgttatg tttaaggtta tgcatcagcg caaagggaat gacgttgatt gcacgcgaca
     8821 tatacaccgc ggtcccgggc acgcgaacag gcggtgattt ctccagcgaa gcaatcatcg
     8881 cttccagaga gttaccatgt tcatgcatcc gccgcagcaa gcggaaacgc tcgctcttcc
     8941 aggtggtcat cacgataaac atcaccgtac cgaggctcaa tggcaaccag ccgccggaga
     9001 gcagtttatc gaggttagcg gtgaacaatg gaatatcgac acaaaggaaa gcaatcaaga
     9061 tcagcgcaac aaaatactta ttccagtgcc agttctgacg tgccacggta gtcgagagaa
     9121 tagacgtcag caccatggtt ccggtcaccg caatcccgta cgccgccgcc aggttgctgg
     9181 agtgctcaaa gctgacaatc acaatcacga ccgcgacata gagcatccag ttcacaaacg
     9241 gaatatagat ttgccctgac tccatttcgg aggtgtgaat aatgcgcatc ggcgacaaat
     9301 atcccagacg taccgcctga cgcgtcaatg aaaagacgcc agagataacc gcctgcgagg
     9361 caattaccgt cgccagtgcg gcgatgatca gcagtgggat cagcgcccag tccggtgcca
     9421 acaggaagaa cgggttctta atcgcttccg ggttctttaa caacaacgct ccctgaccga
     9481 agtaattaag ggtcaaggaa ggtaatacga cggtgaacca cgccaggcga atagggaact
     9541 taccaaagtg ccccatatca gcatacagcg cctcgacccc cgtaatcgac agcaccactg
     9601 cccctaatgc aataaaagaa accgttttgt attcaaggaa gaaatgcacc gcccacatcg
     9661 gattcagcgc atgcagcact tccgggttag caataatgct acgtaacccc agtcctgcca
     9721 gaatcaaaaa ccaggtcagc atgatcggcg caaacagctt accgaccata gccgtgccat
     9781 gcttttgaat cataaataat aaagtgagaa cgataattga gaggggaact atccaggtat
     9841 ccagctgcgg ggcgacgatt tccagacctt caatggctga catcaccgaa atagcgggtg
     9901 ttatgacgac ttcaccatag aaaaagctgc cgccgattag ccccataatc accagcattg
     9961 atgtagttcg cgccgacgta ttacgcccgg caagcgacat caacgtcagg atccccccct
    10021 caccggcgtt atctgcccgc atcacgaagg tgagatattt aatggaaacc acaaagatta
    10081 gcagccagaa gatcagcgat aaaaagccaa acacggcatc gcgttcaacg ccaaaaccaa
    10141 actggccgga caaacattca cgaagtgtat ataacgggct ggtaccaata tcgccgtaga
    10201 caactccaat cgccgcgagg gtaatcgcgg gcaatgattg cttattatca gtgctcatag
    10261 actagtcttt cgttgaaata tgaaatgtgt gcttagtccc ttggcccaca aaaaagcgca
    10321 cagtatgcac gattaacggc aaaatcgtac tcctaaatgc ggccacatta acctggctca
    10381 aagaaaaatc cccccccttt gagactattt tagccaccag caagaaacgt ctatactcgc
    10441 aatttacgca gaacttttga cgaaaggacg ccacttcatt atggctcacc ctcatttatt
    10501 agcggaaaga atttcccgcc tgagcagttc gttggaaaag gggctttatg aacgtagcca
    10561 cgccatccgc ttgtgtttat tagcggcatt aagtggtgaa agtgtgttcc tccttggccc
    10621 gccaggtatt gccaaaagtt tgatcgcccg gcgcttaaaa ttcgcctttc agaatgcccg
    10681 cgcgtttgaa tatctgatga ctcgcttctc cacgccggaa gaggtttttg gtcccctttc
    10741 tattcaggcg ctaaaagatg aagggcgcta tgaacgttta accagcggtt acctgccgga
    10801 agcagaaatc gtctttctgg atgagatctg gaaagcgggt ccggcaattc ttaataccct
    10861 gctcaccgcc attaacgagc gccagttccg caacggtgca cacgtagaaa aaatcccgat
    10921 gcgtctactg gtggcggcct ccaacgagct gccggaagca gacagcagtc tggaagcgtt
    10981 atatgaccgc atgctgattc gtctgtggtt agataaagtg caggataaag cgaatttccg
    11041 ctccatgctg accagtcaac aggatgaaaa tgacaatccg gttccagcct ccttgcaggt
    11101 cacagatgaa gaatatgaac gctggcagaa agagattggt gaaattacgc tgcccgatca
    11161 tgtatttgag ctgattttta tgctgcgcca gcaactggat aaattaccgg atgcgcctta
    11221 tgtctcggat cgtcgctgga aaaaagcgat tcgattattg caggccagcg ccttttttag
    11281 cggtcgcagt gctgttgccc cggttgatct cattttgcta aaagattgcc tgtggtatga
    11341 cgcgcaaagc ctgaatttga tacaacaaca aattgatgta ttgatgaccg gtcacgcctg
    11401 gcaacagcaa gggatgttga ctcgccttgg cgcgattgtg caacgtcacc tgcaactcca
    11461 gcagcaacaa agcgataaaa cagccttaac ggtaattcgt ctgggcggca ttttcagccg
    11521 tcgtcagcag tatcaacttc ctgttaacgt tactgcttcc actctgactc tgctgctgca
    11581 aaaaccgtta aaattgcatg atatggaagt ggttcatatc tcctttgagc gtaacgcgct
    11641 ggaacagtgg ctgagtaaag gtggtgaaat tcgcggcaaa ctgaacggta tcggctttgc
    11701 ccagaaattg aatctggaag tggatagcgc ccaacatctt gttgtacgcg atgtaagttt
    11761 acaaggcagt acgctggcac ttcctggttc atcggctgaa ggtctgccag gtgaaataaa
    11821 acaacaactg gaagagcttg aaagtgactg gcgcaagcaa cacgctttat tcagcgaaca
    11881 gcaaaaatgt ctgtttatcc ctggcgactg gttaggtcgc attgaagcca gcctacagga
    11941 tgtcggcgca cagattcgcc aggcacaaca atgctaacgc tggatacgct taatgtgatg
    12001 ctggccgtca gcgaagaggg attgatcgaa gagatgatca tcgcgctgct ggcctcaccg
    12061 cagctggcgg tcttctttga aaaattccca cgcctgaagg cggcaatcac tgatgatgtt
    12121 ccccgctggc gtgaggcgct gcgtaatcgg ctgaaagatg cccgagtccc gccggaactc
    12181 accgaagagg tgatgtgcta tcagcaaagc cagctcctct ccacgccgca gtttattgtg
    12241 cagctaccac agatcctgga cttactgcat cgtctgaatt ccccatgggc agaacaagcc
    12301 cgacagttgg ttgatgctaa cagcacgatc acttcagcgt tacacacgct tttcctccag
    12361 cgttggcgtt taagtctgat cgtgcaagca acgacgttaa atcaacagtt attagaagaa
    12421 gaacgcgaac aactgttgag tgaagttcag gaacgcatga cgcttagcgg acaacttgaa
    12481 ccgattctcg cagataacaa taccgcagct ggtcgtctgt gggatatgag cgccggtcag
    12541 cttaaacgtg gcgactatca gttgattgtg aaatacggtg aatttcttaa cgaacagccg
    12601 gaactgaaac gcctggcaga acagctgggg cgttcccggg aagccaaatc aataccgcgc
    12661 aacgatgcgc agatggaaac cttccgcacc atggtgcgcg aaccggcgac ggttcctgag
    12721 caggttgatg gtctgcaaca aagcgatgat attttacgtc ttctgccgcc agaactggcg
    12781 acactaggga taacggaact ggagtatgag ttttaccgtc ggctggtgga aaaacagttg
    12841 ctcacctatc gcctgcacgg tgagtcgtgg cgtgaaaaag tgatcgaacg cccggtggtg
    12901 cataaagatt acgacgaaca gccgcgcgga ccgtttattg tctgcgtgga tacttccggc
    12961 tcaatgggcg gctttaatga acagtgtgca aaagcgttct gcctggcctt gatgcgcatt
    13021 gctctcgctg aaaaccggcg ctgttatatt atgctatttt ccaccgagat cgtccgttat
    13081 gagctttcag gcccacaagg catcgaacaa gcaatccgtt ttttaaacca gcagtttcgt
    13141 ggcggcaccg atcttgccag ttgttttcgc gccattatgg aacgcttgca aagcagggaa
    13201 tggtttgatg ccgatgcggt ggtgatttct gattttatcg cccagcggtt gcctgacgac
    13261 gtgacgagta aagtgaaaga gctgcagcgg gtacatcagc atcgctttca tgccgtggcg
    13321 atgtcggcac acggcaaacc cggcatcatg cgcattttcg atcatatctg gcgctttgat
    13381 accgggatgc gaagccgcct gctcagacgc tggcggcgat aaattattac agcagagaag
    13441 ggacgctctc gcgaacagca gctggccata ctccacactg aacctggccg atatgcggca
    13501 gttgcagcag cagcatggtc aaacgagact ggccgatacc gccgccgatg gtctgcggca
    13561 tttcaccgcg cagcaacgcc tgatgccatt ccagctgcag acgatcttca tcaccggtca
    13621 gcgccaactg atgcttcaac gtgtcggcat ccacgcggat ccccatggaa gaaagctcaa
    13681 acgcatcttc cagtaccggg ttccacacca gaatatcgcc gttcagaccc gcatggccca
    13741 gctctgacgg ggtgctccag tcatcataat ccggtgcgcg cacgtcgtgg cgatgaccat
    13801 cgctcagctt gccgccaatc ccgacaagga ataccgcacc aagatctttc gctatcgccc
    13861 gctcacgccc tttggcatca agatccggat aacgagacag taactcctgg ctgtgtacga
    13921 agtggatctg atccggcagg aacggtgcca ggccaaactc ttcgctaacc gcagcttcgg
    13981 ttgctttaat tcccgcccag atcgcctcta ccgtgctttt cagagtcgag aattgacgct
    14041 caccgtcgcc cattacgcgt tcccagtccc actggtcaac atagaccgag tgcaacggag
    14101 aaagacggtc ttcatcgggg cgaagggctt tcatgtgcgt gtacagccct tcgcccgcgc
    14161 tgaagtcgtg ttgccctaag gtctgacgtt tccacttcgc cagtgaatga accacttcga
    14221 actgggcatc aggcagagct ttcactttta cctgcaccgc tttttcacag cccgacaagt
    14281 tatcctgcgt gccatccccc acacggctaa gaatcggcgc ctggacttcg atcagcccca
    14341 gacgttcttc cagttggcga gaaaagtgag atttcacgaa gctaatttgg cgttgtttgg
    14401 caatgtaagc ggttttcatt tttttatact cctgcgtcct gttgcttatg attaagcaac
    14461 aaaaaccgac acacatgcaa taatcattca ataaaaagcc taacacactt ttgattcatt
    14521 aaaaaagaag gctaaaatag aatgaatcat caatctgcat aagaaaatcc tatggaaaat
    14581 tatctgatcg acaatctgga ccgtggcatc ctggaagcat taatgggcaa tgcgcgcacc
    14641 gcttacgccg aactggcgaa acaatttggc gtcagtccag ggacgattca cgttcgagta
    14701 gagaaaatga agcaggcggg gatcattacc ggggcgcgta ttgatgtcag cccgaagcag
    14761 ctcggttatg acgtaggctg ctttatcggc attatattaa agagcgccaa agactatcct
    14821 tccgcgctgg cgaaactgga aagcctcgat gaagtcactg aagcctatta cacaaccggc
    14881 cactacagca tctttataaa agtgatgtgc cgttcgatcg atgctctcca gcatgtactt
    14941 atcaacaaga tccaaacaat tgatgaaatt cagtccaccg agacactgat cgtcctgcag
    15001 aacccgatca tgcgtaccat caagccctga tcggcttttt taatcccata cttttccaca
    15061 ggtagatccc aacgcgttca cagcgtacaa tacgccactc ttaataaagg tggcggttta
    15121 tggcagatat cactcttatc agcggcagca ccctcggcgg tgccgaatat gtagcagaac
    15181 acctggctga aaagctggaa gaggcgggtt ttaccaccga aacgctgcac ggtccgctgt
    15241 tggaagattt atctgcctcg gggatctggc tggttatcag ctcaacccac ggtgccggag
    15301 atattccgga caacctttct cctttctatg aagcattgca ggaacagaag cccgatcttt
    15361 ctgcggtccg ctttggcgca atcggtattg gtagtcgtga atatgacacc ttttgtgggg
    15421 ctattgataa actcgaggct gaactcaaaa attccggtgc aaaacagacg ggcgaaacac
    15481 tgaagatcaa cattcttgat cacgacattc cggaagatcc ggcagaagaa tggctgggat
    15541 cgtggattaa tttactcaaa tgagtataca gatcgtgcga tctactgtgg ataactctgt
    15601 caggaagctt ggatcaaccg gtagttatcc aaagaacaac ggttgttcag tttttgagtt
    15661 gtgtataacc cctcattctg atcccagctt atacggtcca ggatcaccga tcattcacag
    15721 ttaatgatcc tttccaggtt gctgatctta aaagccggat ccttgttatc cacagggcag
    15781 tgcgatccta ataagagatc acaatagaac agatctctaa ataaatagat cttcttttta
    15841 atacccagga tcccaggtct ttctcaagcc gacaaagttg agtagaatcc acggcccggg
    15901 cttcaatcca ttttcatacc gcgttatgcg aggcaatcac catgttttat ccggatcctt
    15961 ttgacgtcat catcattggc gggggtcatg caggcaccga ggccgcgatg gctgcggcgc
    16021 gtatgggtca acagactctg cttttgacac acaatatcga cactctgggg cagatgagct
    16081 gcaacccggc gatcggaggt attgggaagg gacatctggt aaaagaagtg gatgcactcg
    16141 gcggtctgat ggcgaaagcg atcgatcagg cgggtatcca gtttaggata ctaaacgcaa
    16201 gcaagggacc ggcagttcgc gctacccgag ctcaggcgga tcgtgtgctc taccgtcagg
    16261 cggtacgtac ggcgctggag aaccaaccga atctgatgat cttccagcag gcggttgaag
    16321 atcttattgt cgaaaacgat cgcgtggtcg gtgctgttac ccaaatggga ctgaagttcc
    16381 gtgccaaagc cgtcgtgctc accgttggga cgttcctcga cggtaaaatt catatcggtc
    16441 tggataacta cagcggtggc cgtgctggtg atccgccgtc cattccgctt tctcgccgtt
    16501 tgcgtgaact gccgctgcgc gttggtcgtc tgaaaaccgg gacaccaccg cgtattgatg
    16561 ctcgaaccat cgactttagc gtactggcgc aacagcatgg cgataaccca atgccggtat
    16621 tctcgtttat gggcaatgcg tcccagcatc cccagcaggt gccgtgttat atcactcata
    16681 ccaacgagaa aacccatgat gtgatccgca gtaacctcga tcgtagccca atgtacgcag
    16741 gggtgatcga aggtgtcggc ccacgctact gcccgtcgat cgaagacaaa gtcatgcgct
    16801 tcgccgacag aaatcagcat cagatcttcc ttgaaccgga agggctgacc tctaacgaaa
    16861 tttatccgaa cggtatctcc accagcctgc cgttcgatgt gcagatgcaa atcgtccgct
    16921 ctatgcaggg gatggaaaac gcgaagatcg tgcgtccggg ttatgccatt gagtatgact
    16981 tcttcgatcc tcgcgacctg aaaccgacgc tggagagcaa gtttatccag gggctgttct
    17041 ttgctggtca gattaacggc actaccggtt acgaagaagc cgctgcgcaa ggtttgctgg
    17101 ccggtcttaa cgctgcccgt ctgtctgctg acaaagaagg ttgggctccg gcgcgttctc
    17161 aggcgtatct cggcgtactg gttgatgacc tgtgcacttt aggaaccaaa gaaccgtatc
    17221 gtatgtttac ctcgcgcgca gaatatcgtc tgatgctgcg cgaagataat gcggatctgc
    17281 gtttgactga aatcggtcgt gaactgggcc tggtggatga cgaacgttgg gcgcgcttta
    17341 acgagaaact tgagaatatc gagcgtgagc gtcagcgtct gaaatcgacc tgggtaaccc
    17401 cgtcggcgga agctgcagcc gaagtgaatg ctcacctgac tgcgccgctt tcccgtgaag
    17461 ccagtggtga agatctgctg cgtcgtccgg aaatgactta tgaaaaatta accacgctga
    17521 cgccgtttgc ccctgcgttg acagacgaac aggcggcgga acaggttgag attcaggtta
    17581 aatacgaagg ttatatcgcg cgccagcaag atgagatcga aaagcagctg cgtaacgaga
    17641 acaccttgct acccgcgaca ctggattacc gccaggtatc cggtctttct aacgaagtga
    17701 tcgccaaact taacgatcac aaaccggcct ctatcggcca ggcttcgcgt atttctggcg
    17761 tcacgcctgc ggccatctcc attctgctgg tgtggctgaa aaaacagggt atgctgcgtc
    17821 gtagcgcata acgcattaaa aatgcctggt aagcacccgc ttaccaggca acgcatcaag
    17881 aacaggtaat caccgtgctc aacaaactct ccttactgct gaaagacgca ggtatttcgc
    17941 ttaccgatca ccagaaaaac cagcttattg cctacgtgaa tatgctgcat aaatggaaca
    18001 aagcgtacaa cctgacttcg gtccgcgatc ctaatgagat gctggtacgc catattctcg
    18061 atagcattgt ggtggcaccg tatctgcaag gtgaacggtt tatcgatgtc ggcaccggtc
    18121 ctggactgcc tggcattcca ctctctatcg tgcgtcctga agcccatttc actctgttgg
    18181 atagccttgg taaacgcgtg cgtttccttc gtcaggtgca acatgagctt aaactggaga
    18241 atattgagcc agtacagagc agggtagaag agtttccttc agagccgcca tttgatggcg
    18301 taattagccg cgcttttgcc tctctgaacg atatggtgag ctggtgtcac catcttcctg
    18361 gtgagcaagg ccgtttctac gcgctgaaag ggcaaatgcc ggaagatgaa atcgctttgt
    18421 tgcccgaaga atatcaggtt gaatcagtgg ttaaacttca ggttcctgcc ctggatggcg
    18481 aacgccatct ggtggtgatt aaagcaaata aaatttaatt tttatcaaaa aaatcataaa
    18541 aaattgaccg gttagactgt taacaacaac caggttttct actgatataa ctggttacat
    18601 ttaacgccac gttcactctt ttgcatcaac aagataacgt ggcttttttt ggtaagcaga
    18661 aaataagtca ttagtgaaaa tatcagtctg
//
LOCUS       DAZUCE010000089        18566 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZUCE010000089 DAZUCE010000000
VERSION     DAZUCE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.089"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            178..1077
                     /gene="yegS"
                     /locus_tag="ACHY03_002585"
     CDS             178..1077
                     /gene="yegS"
                     /locus_tag="ACHY03_002585"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFS3905798.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIVGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1159..1932)
                     /locus_tag="ACHY03_002586"
     CDS             complement(1159..1932)
                     /locus_tag="ACHY03_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS3905799.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2038..3078)
                     /gene="gatD"
                     /locus_tag="ACHY03_002587"
     CDS             complement(2038..3078)
                     /gene="gatD"
                     /locus_tag="ACHY03_002587"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS3905800.1"
                     /translation="MKSVVNDTDGIVRVAESVFPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHSGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQAVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3126..4481)
                     /locus_tag="ACHY03_002588"
     CDS             complement(3126..4481)
                     /locus_tag="ACHY03_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFS3905801.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4485..4769)
                     /gene="gatB"
                     /locus_tag="ACHY03_002589"
     CDS             complement(4485..4769)
                     /gene="gatB"
                     /locus_tag="ACHY03_002589"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFS3905802.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4800..5252)
                     /gene="gatA"
                     /locus_tag="ACHY03_002590"
     CDS             complement(4800..5252)
                     /gene="gatA"
                     /locus_tag="ACHY03_002590"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFS3905803.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5262..6524)
                     /gene="gatZ"
                     /locus_tag="ACHY03_002591"
     CDS             complement(5262..6524)
                     /gene="gatZ"
                     /locus_tag="ACHY03_002591"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFS3905804.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRTYRYGCAE"
     gene            complement(6553..7407)
                     /gene="gatY"
                     /locus_tag="ACHY03_002592"
     CDS             complement(6553..7407)
                     /gene="gatY"
                     /locus_tag="ACHY03_002592"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFS3905805.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFTQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7716..8768)
                     /gene="fbaB"
                     /locus_tag="ACHY03_002593"
     CDS             complement(7716..8768)
                     /gene="fbaB"
                     /locus_tag="ACHY03_002593"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFS3905806.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9025..10302
                     /locus_tag="ACHY03_002594"
     CDS             9025..10302
                     /locus_tag="ACHY03_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFS3905807.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLITAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10299..11303
                     /locus_tag="ACHY03_002595"
     CDS             10299..11303
                     /locus_tag="ACHY03_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFS3905808.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRGEFTDDTSMALCLADALLEREGRIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPASDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11300..12265
                     /locus_tag="ACHY03_002596"
     CDS             11300..12265
                     /locus_tag="ACHY03_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFS3905809.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12239..12985)
                     /locus_tag="ACHY03_002597"
     CDS             complement(12239..12985)
                     /locus_tag="ACHY03_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS3905810.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13037..13855)
                     /locus_tag="ACHY03_002598"
     CDS             complement(13037..13855)
                     /locus_tag="ACHY03_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFS3905811.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAICPRQTVNFFYSTAVQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(13920..14720)
                     /gene="thiD"
                     /locus_tag="ACHY03_002599"
     CDS             complement(13920..14720)
                     /gene="thiD"
                     /locus_tag="ACHY03_002599"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFS3905812.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14717..15505)
                     /gene="thiM"
                     /locus_tag="ACHY03_002600"
     CDS             complement(14717..15505)
                     /gene="thiM"
                     /locus_tag="ACHY03_002600"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFS3905813.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVATGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPIMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15540..15636)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15728..16000)
                     /gene="rcnR"
                     /locus_tag="ACHY03_002601"
     CDS             complement(15728..16000)
                     /gene="rcnR"
                     /locus_tag="ACHY03_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFS3905814.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16121..16945
                     /gene="rcnA"
                     /locus_tag="ACHY03_002602"
     CDS             16121..16945
                     /gene="rcnA"
                     /locus_tag="ACHY03_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFS3905815.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17164..17502
                     /gene="rcnB"
                     /locus_tag="ACHY03_002603"
     CDS             17164..17502
                     /gene="rcnB"
                     /locus_tag="ACHY03_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFS3905816.1"
                     /translation="MTIKYKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLINNTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17584..>18566)
                     /locus_tag="ACHY03_002604"
     CDS             complement(17584..>18566)
                     /locus_tag="ACHY03_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3905817.1"
                     /translation="WAGCRTTASLNITDGINVGEILANETSFSKSVVFTGISCDTSTD
                     KIVYKNIQSDWVEVGPFGNGEKLKVKIESLGKTSDTIGKSSNAQAVLPYVVKIARGTP
                     DFTGERKSTWFISDTVIANIGGESSSSIDFWLGICKALKFNWCVNYLTSKLAGDTFTL
                     GLSISYYPKNTTCKPENTVIKVDDIALFQLRNQGKIAANSKEGTITLKCDNLFGDKKQ
                     ASRNMVVYLSSSDLVKGSNTILRGKTDNGVGFVLDLTEPPKGTEAAIKISANGDQGAA
                     TSLWKTDKPGVSLNSNIINIPVMASYYVYDEKKVKSGALEATALINVKYD"
BASE COUNT         4597 a         4795 c         4420 g         4754 t
ORIGIN      
        1 atttatcggt ggctaccgtt aatattcgct gttcccattg caagctcctg gtggtaacca
       61 ctgaatcccc catacttgaa ctgacttttt atcctccgac tttcatcctg ttctgactcc
      121 accttttctt ttctgctcta cactatctac agaccaatca taaaggcaca tacgatcatg
      181 gcagaatttc ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg
      241 cgcgaagcaa ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg
      301 gagaaaggcg atgccgcacg atatgtagag gaggcccgga agttgggcgt cgcaacggtg
      361 attgccggtg gtggcgatgg caccattaat gaagtttcta cggcgttgat tcagtgtgag
      421 ggggatgaca tacccgcgct gggaattttg ccattaggaa ccgccaatga ttttgctacc
      481 agtgtaggga ttcctgaggc actggataag gcgctgaaac tggcaattgt cggtaacgcc
      541 attgcgatag atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc
      601 ggatttggga cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtggc
      661 gtctcttaca tcattcatgg cttaatgcgc atggatactc tgcaaccgga ccgttgtgaa
      721 atccgcggtg aaaactttca ctggcaaggt gacgccctgg tcattggtat tggtaacggg
      781 cgtcaggccg gtggcggtca acaattgtgc ccgaacgcgt taattaacga tggcttgctg
      841 caactgcgca tttttaccgg cgatgaaatt cttccggctc tcgtatcaac cttaaaatct
      901 gacgaagata acccgaatat tatcgaaggc gcttcatcgt ggtttgatat acaagcccca
      961 cacgaaatca cttttaatct tgatggcgaa ccgttgagcg gacaaaactt tcatattgaa
     1021 atacttccgg cggcgttgcg ttgtcgatta ccaccggatt gtccattatt gcgttaatca
     1081 aactattctc tcttgaacca gggagaatcc gtaatgccgg atgcggtgta aataccttat
     1141 ccggcataca gaacaatact acaccgtaat taattttacc cgtgtcttct gcaatgccag
     1201 tttatccacc tctgacacag catcgtcggt aataataata tcgatttttt cgattggcag
     1261 tacctgatta aaaccgcgac ggttaaattt cgatgaatcg agaacggcaa tcactttatt
     1321 agcggcggtg accatcgccc cgctaatcga ataaccttca ttaaaggtcg taataccatt
     1381 aaccgcatca ataccatcag caccgacaaa cattaaatcg gcattaatat cctgcaatga
     1441 acgctcggca atggaaccat gcatcgagcg cgttttatgg cggacggtgc caccacagac
     1501 gaccagagta atatctttat tttcggaaag ggcaaacgct gccgggagac tgttggtaat
     1561 caccgtgata tttttggcgg tcattaatcc ttcggcgata agcattgtcg tacttccgct
     1621 gtcgagaata acggtcatcc cttcgtggat cattttgact gccgcctgcg ctattctgtt
     1681 ttttggcgcg ctggcgagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc
     1741 actattaccg gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg
     1801 ttcgagaaag cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag
     1861 atcctgaaca agcacggttc cctgttcatt cactaattgg atgatcttat tccttcgctc
     1921 gaatgaattc atcgtcattt acccatattc aattgtggct agtgtaaacg aagggggacc
     1981 attggtgaat gatctgtttt gcacaatacc gggccagcat cacgctggcc cgcggtttca
     2041 gggaatgagc aacactttgc ccggcatagc attacgagcg atgtcacgca ccgcctgggc
     2101 gaagctttca aagcttccac ggtgagcgat taatggctcc agacttaact tacgttctgt
     2161 cagcaaccgg ctcgccgttt cccactcctg ccccggccaa gggctggagt agttcatcca
     2221 actgccgata accgtcagct ctttacgcaa tattttgcca aacgttgccg atgttaaatg
     2281 cagatcctga tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg caatctctac
     2341 tgccagttcg acagtttgcg gtacgccagc cgtctcgagg ataagctgat taaagcgcag
     2401 ctcgcgtaaa acggcctgca tttgcggcgc gctcatttcg ctactgttaa atgtttgcat
     2461 cgcaccgaaa gattttgcca gtgccagttt ttctgaacta atgtcgatag ccgtcacact
     2521 ctttgctccc agcgcgacag cgcactgaat ggccagcagg ccaatggttc cggcaccaat
     2581 aataataacg tttttattct cacaaccttg cgctaaatga aaagcatgca ggccaacggt
     2641 aatcggctca ataaaagccc catcctcaat aggcatatcc gtgggtagag caaagacatt
     2701 ttttcgctta acgacaatat attcagcaaa tccaccatca cgccgcgagc caataaaatc
     2761 atattttgcg cactgggaat aaaacccttt caaacactct ggacaagtaa aacagggtaa
     2821 taacggcaca caggcaaccg catcgccaga gtgtaaatca tcaacaccgg atcccaccgc
     2881 atcaatatag ccgctaaatt catggcctaa cgttattgga taataatgtg caccattttt
     2941 aaatatcctg ggtaaatcgg aaccacataa gcccgagctg gcaattttta cccgaacctc
     3001 atcctgatgt ttaatttcag gaaagacgct ttctgcaacg cgcacgatac catcagtatc
     3061 attcaccact gatttcataa aaactcctga ttgttaaggg ggataacctc cccctcaggt
     3121 aaaaattatt ctgcgagaac gactttctct tgtttaataa agccacgcgc tctacgccag
     3181 gtcatgaaaa taccggtaag ataaattgcg ccgataataa tgaaaccggg aatattttgc
     3241 ggggagaaaa cctgaatcag taaccaggta atgggagaac cgccctgatc cattgaagcc
     3301 accatacccc cggcttttaa cgccccagca ttagccgcca gttgggtgtg caaaccaata
     3361 gtttgcgtcg cgatccacag ggtgatgctc ataatgatga cacccgagat taaggtgcgg
     3421 aacagatttc cacgatgcac cgcgaccgcc atcgccacaa agaagccgat agtggcaaga
     3481 tcgccaaacg gcagcacctg attacccggc acacaaacag caattaaaat ggtgagcggg
     3541 ataaaaatca ggcttgccga taccaccgcc gtatgcccca gcagtaatgc gggatccagg
     3601 ccaatcagga actcctgacc gccgaacttc gcctgtaaac gactacgggc ctgcttagcg
     3661 atgggcgtta aaccatccat gatgggttta atcacccgtg gcattagcag catcaccgcc
     3721 gccgttttta ccgccagctg caatacacct ttgacatcgt aacccgcgag gatgccgata
     3781 atcagcccca tcacaaaacc cacggtgaca ggctcgccaa aagggccaaa tttgcgctga
     3841 atatcatcgg cgctaaattt aattcggtta acgcctggga ttttctcgat aatggcatcg
     3901 accagcaccg caatcggccc catatacgcc gacgtaccgt gcgggattgc aatgccttcc
     3961 agctcaaaga aatttcgggt atcgcgggca aaccagtcgc cgagcttata aacaaacgcc
     4021 gcgtgaatta ccacacccgc catccctatc atccatgaac cggttgccag atgcagcaac
     4081 gcgccggtga aggtcatatg ccagatattc cagatatcaa catttaccac ccgcgtcata
     4141 cgggtcagta acatcgccac gttaaccaga atcgcaatcg gaatcgccac cagcgcaatt
     4201 tgcgacgccc aggtcattgg tgaagagccc ggccagccga catcgaccac atgcagattc
     4261 aggtcgaaat tttccgccat cgctttcgcc gccggaccaa tggaatccag cattaagcca
     4321 atcacaaggc caatgccaac aaagccaatc ccgatatgca gacccgcttt aaagcaatcg
     4381 cctgccttca tgcctaaaat tttagaaaaa ataatgatga caatcggcaa catcaccgta
     4441 gggccgagat cgagaatata acgcatgact tctgaaaaca taggtcaccc ctgtaagata
     4501 gtcagaattt tattttgtaa tgcttcgata ccgacaccag aaacaaaagg catgccgtga
     4561 actaacggaa tatcgccaaa actacggtca actttggcgg tagtacatat caaatgcacg
     4621 ccatccatat aggtttctat ttcattaacc cgacactgga ttaattcaac aggaatatta
     4681 tgactctgac acaactcttt aatttcttcc gccgccatcg tagaggtcgc aaccgcgcct
     4741 ccgcaagcga caataatctt gcgtttcata aatagtcctt tttattgtta cagagggatt
     4801 tattcgtctg aatctaaaac atactttgtg aagtattcct ttaactgggt ttcaggaaga
     4861 gtgattagtg tctcgacgat atcgggctgt tgtaacttgc caaataaaca gcgtaaaagt
     4921 ttcaattgct gctgcggatt ttccacaatc aacgcaataa ccaacgatac cgccacgtcg
     4981 ttatcatcat ccgcttgctg aaaatgaact ttatttgttg gccttaacag ataaatggct
     5041 gacgacttag catgaatcgc ctcacaatgc ggtattgcaa tggcgtgctg ctcaagcatt
     5101 atcccagtag ggaattctgc ttctctggca attaatgcct gtggccaggt atcatgaacc
     5161 acacctttcg cgagcatctc attaccgata tgggttaaaa cttcgctacg atcgacaaaa
     5221 gaaattccgc tacgaacaaa caggttagtc atataccgtc cttattccgc acagccgtag
     5281 cgataggtgc gcaaaacatc ataaatttta tccataataa gctgatgcgg tattgctgat
     5341 aattccccgg actgaatgcg ttcaaattgt ttaggaagat actgactaat catgcccagt
     5401 gggatgtcca cgccttccag attcaccatc atcgtttcga cgctattttt aatccgacta
     5461 tgcggccagt aataacgaat acgatccgac aggctgtaac gaatatccag taatgaatcg
     5521 ttaaaacccg tgcgataata tttcttccag tactgcggtt cgtcgagcat cacttcttca
     5581 attaccgcca ggcaaccgct gcgattttcg ggggcgataa gttcctgctc aatttgtgcc
     5641 agcgcaaata tcgcctcacg taaagcaaag gttaatgcgg gaccgacttt caatattgca
     5701 aagtgatcgc ggactaattc ccaataagcc gtccgggtct ggtaatcggt agaatgtgct
     5761 tcataaacca ttcgggtgtt ttctatccat tgcgccagcg cctgcgcttc ctgcggctga
     5821 taatggataa tattgctgtg atcaaattcc acacccggct gcaccacgat ggcaatcacg
     5881 cgtgttaacg cctctgtcag cccacgggca ataaaggcct tttgatgcgt acgtaaagta
     5941 ttggcggcat cttcaacatg ggtgatgtgt actgactgaa tggcgctggc ctcaccgccc
     6001 ggaaccggaa cttcggtgcc aatgacatag ctcagttgct cacgctggca atctgtcgcc
     6061 acactttccg cagcaaagca aagcacagca gctcgttccg caaccgtttc tggtgctaac
     6121 ggtatgggat cccccgcgca ggacattgac gcatcaagat gaattttact gaagccagca
     6181 cgaacatatg cttttaccag ctcgacggat ttttccattg ccgcatccgc attttcttgc
     6241 tgccagcagt ttggccccag atggtctccg ccgagaataa tgcgctcgcg cgcaaatccg
     6301 actttatcgg caatcgtaaa aacaaattcg cgaaagtctg ccggtgtcat tccggtataa
     6361 ccgccaaatt gattgacctg gtttgacgtt gcttcaatca gcactttgcg cgtgctgttg
     6421 cgatcaaatg ccagcgccgc ttcgataacc aacggatggg cagaacagac tgaacatatg
     6481 ccgatatgtt caccagcttt atgccgggca attaacgttt tcatgttttt tccttgttaa
     6541 atggcaggtg cgttatgccc tgccctcgca gccacaatcg gcaatcactt tgctcaccac
     6601 atcgcgcatt gcggatttag ccgactgcaa ataatcccgg ggatcggtcg cttcggggtg
     6661 ctcggtcagg taatttttta acgcctgcgt gaaggcattt ttcagctccg ttgcaacgtt
     6721 gattttgcat atccccagtt tgatggtttg ctgaatatcc ttagtcgata accctgacgc
     6781 gccatgcagc accagcggta agttcaccca ctggcgaatg ttctccagtc gagaaaaatc
     6841 aagcgccggt gcgctggcat acataccatg agccgtgccg atggcgaccg ccagggaatc
     6901 aatgccggtt gcctcggcaa attcacgcgc ctgagcgggg ttggtataaa aagcatcggc
     6961 ttcattgact tgcacatcat cttcctggcc gccaagttgc cccagctccg cttcgacgct
     7021 gacatcaaag cgatggcaaa aatccaccac ctctttgacc cgtgaaatat tttgcgcaaa
     7081 aggcaaatgc gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct tctgagcgat
     7141 atcgtcaaat ttcgtgtgat ggtcgagatg aattgccagt ggatggtgat attgcttcgc
     7201 catcgcgctg accagcgcca acagattttc tgtaccagca tgagtaaatg tgccaggcgt
     7261 tccggcgatg atgaccggcg catgcaggtt ggcagcggtt tccaccacca cttgcatcgt
     7321 ttcgagattg tgaatattga atgccggaac cgcgtaaccg ccgcgctgcg cgttgttcag
     7381 catctgcttt gtcgatacca cgtacatttt cacatcctgt cgtttgcttt cgatttcaaa
     7441 acataatgaa attatttgtt tttaaatgtc gagataacga tcacaaaaac gacaatatga
     7501 aaattgttag aggagtgaaa ggcaaaaaaa acggcctccc gatagggaag ccgtagcaaa
     7561 gtgcgcgtta ttttatgccg gatgcagtgt aaacatacgt ggcagcgctg tgtaggcatg
     7621 ataagacgct tcagcgtcgc atcaggcata ggttgccgga tgcagcgtaa acgccttatc
     7681 cgtcctacgg ggtggtgttg ttcgtataag gcgtatcagg cgatagtaat tttgctatcg
     7741 agataaacgt cctgcacggc gttaatcagt ttcacgccgt cagccatcga tttcttgaac
     7801 gccttacgtc caagaatcag ccccattccg ccagcgcgtt tgttgataac cgcagtacgc
     7861 actgcatcgc tgaggtcagt ttcaccgcct gcagcaccgc cggagtttat caacccggcc
     7921 cggcccatat agcagttagc taactgataa cgcaccagat caatcgggtt ttcgctggtt
     7981 aacttgctgt acacgcgatc gtcggtataa ccgtaattaa ttgctttata gccgccgtta
     8041 ttttccgcca ttttttgttt gacgatatct gcacctatgg ttgccgccag atggttcgcc
     8101 tgaccagtca ggtcggcgga gacatggtaa tcaacgccat ctttcttaaa ggcggagtta
     8161 cgcaaatagg cccacagcac tgtcaccatg cccagctcgt gcgcacgttc aaaagccgca
     8221 gaaatttctt caatctggcg acgtgactct tccgagccaa aatagatagt cgcaccaacc
     8281 gccaccgcgc ccatgttgaa ggcctgctcc acgctggcat acagcgtttg atcgtaggta
     8341 ttcgggtaac ttagcgtctc gttgtgatta agtttgacga gaaatggaat gcgatgcgca
     8401 tagcgccgcg ataccgacgc caacacgccg taagttgatg ccacacagtt acagcccgct
     8461 tcgatcgcca gttcaacaat gtttttcggg tcaaagtaga gcgggttagc agcaaatgaa
     8521 gctccggcag agtgctcaac gccctggtca acgggcagaa tagaaagata tcctgtgcca
     8581 gccagacgcc cggtgttgta caacgtctgc atattacgta acaccgctgg cgggcgatta
     8641 ttgtcaatca tcacgcggtc tacgtagtca tgtccgggga gataaagctg gtcagaagga
     8701 atagtcatac aacggtgctg taaaaggttg tcggcgtctt tgccaagcaa ctgcgcaata
     8761 tctgtcatta ctatgctccc gtaaattccg attggatatc ggctatggat tgtcctggcc
     8821 cgccttttgc gggcaatcat aatcctggtc gttacgggcg agtttttcca tcttttggat
     8881 gttttttcag cgttttctgt tggctcgatt catcagaaaa aatgttagcg cggtcaagtt
     8941 ttcaccgcaa aggtatttaa aaggtattat taagtggtat tgtcatcgcg taccttacat
     9001 tacctgtcat gaaggaatta aaagatgaaa acaacagcaa agctgtcgtt catgatgttt
     9061 gttgaatggt ttatctgggg cgcgtggttt gtgccattgt ggttgtggtt aagtaaaagc
     9121 ggttttagtg ccggagaaat tggctggtcg tatgcctgca cagccattgc ggcgatcctg
     9181 tcgccaattc tggttggctc catcactgac cgctttttct cggcgcagaa agtgctggcg
     9241 gtattgatgt tcactggtgc ggcgctgatg tatttcgctg cgcaacagac cacttttgcc
     9301 gggttcttcc cgttactgct ggcctactcg ctaacctata tgccgaccat tgcgctgact
     9361 aacagcatcg cttttgccaa cgtgccggat gttgagcgtg atttcccgcg cattcgtgtg
     9421 atgggcacta tcggctggat tgcctccggt ctggcatgtg gtttcttgcc gcaaatgctg
     9481 ggttatgccg atatctcacc gactaacatc ccgctgctga ttaccgccgg aagttctgct
     9541 ctgctcggtg tgtttgcgtt tttcctgccc gacacgccac caaaaagcac cggcaaaatg
     9601 gatattaaag tcatgctcgg cctggatgcg ctgatcctgc tgcgcgataa gaacttcctc
     9661 gtctttttct tctgttcatt cctgtttgcg atgccactgg cgttctatta catctttgcc
     9721 aacggttatc tgaccgaagt tggcatgaaa aacgccaccg gctggatgac gctcggccag
     9781 ttctctgaaa tcttctttat gctggcattg ccgtttttca ctaaacgctt tggtatcaaa
     9841 aaggtattat tgcttggtct gatcaccgct gcgatccgct atggcttctt tatttacggt
     9901 agtgcggatg aatatttcac ctacgcgtta ctgttcctcg gtattttgct tcacggcgta
     9961 agttacgatt tttactacgt taccgcttac atctatgtcg ataaaaaagc ccccgtgcat
    10021 atgcgtaccg ccgcgcaggg gctgatcacg ctctgctgcc agggcttcgg cagtttgctc
    10081 ggctatcgtc ttggcggtgt gatgatggaa aagatgttcg cttatcagga accggtaaac
    10141 ggactgactt tcaactggtc cgggatgtgg acttttggcg cggtgatgat tgccattatc
    10201 gccgtgctgt tcatgatttt tttccgcgaa tccgacaacg aaattacggc tatcaaggtc
    10261 gatgatcgcg atattgcgtt gacacaaggg gaagttaaat gaaaacagaa cgtattctcg
    10321 gtgctcttta tgggcaggcg ttaggggatg cgatggggat gccctccgag ctttggccac
    10381 gcagccgcgt taaagcacac tttggctgga ttgaccgttt tctgcctgga ccaaaggaga
    10441 ataacgcggc ctgttatttt aaccgcggcg aatttaccga tgacacctcg atggcgctgt
    10501 gtctggcgga tgcgttactg gaacgtgaag gcaggatcga cccgaatctg attgggcgta
    10561 atattctcga ctgggcgctg cgtttcgacg cctttaacaa aaacgtacta ggtccgacct
    10621 cgaagattgc tctcaacgcc attcgcgacg gtaaacccgt tgctgaactg gaaaacaacg
    10681 gcgtgaccaa cggcgcagcg atgcgcgtct cgccattagg ttgtttgctt ccggcgagtg
    10741 atgttgattc ctttattgat gatgtggcgc tggcgtccag cccgacccat aaatccgatc
    10801 tggcagttgc aggtgcggta gtcatcgcat gggcgatttc tcgtgccatt gacggagaaa
    10861 gctggtcagc gattgttgat tcactgcctt caattgcccg acatgcacag caaaaacgca
    10921 tcactacctt cagcgcctca ctggcagcac gtctggagat tgcgctgaaa attgtgcgca
    10981 atgccgacgg caccgaatcc gccagcgaac agctttacca ggtcgttggc gcaggtacca
    11041 gcactattga gtccgttccg tgcgccattg cgctggttga actggcacaa accgacccga
    11101 atcgctgcgc cgtcctgtgc gctaaccttg gcggcgacac agacaccatc ggcgctatgg
    11161 cgacggcgat ttgcggcgcg ttgcatggcg ttaacgctat cgatcctgca ttaaaggcgg
    11221 aactggatgc ggtaaatcag cttgatttca accgctatgc cacagcgctg gcgaaatatc
    11281 gtcaacaacg ggaggcggta tgagcggcgc tcgattacac acgctgctgc ctgaattaac
    11341 ctcgcgtcag ccggtgatgg tcgtcggcgc ggcggtcatt gatgtgatcg ccgacgctta
    11401 tgccctcccc tggcgtgggt gcgatatcga actgaagcag cagagcgtta acgttggcgg
    11461 ctgcgcactg aatattgccg tggcgttaaa gcgcctcggc atcgaagcgg gtaatgcctt
    11521 gccgctcggt cagggcgtgt gggcggagat tattcgcaat cggatggcga aagaggggtt
    11581 aatcagcctg atcgataacg ccgaaggcga taacggctgg tgcctggcgc tggttgagcc
    11641 ggatggcgaa cgtactttta tgtcattcag cggtgtggaa aatcagtgga atcgccagtg
    11701 gctggcgcga ttaaccgttg cgcctggcag cctgctctat ttttccggtt atcaactggc
    11761 ctcgccctgc ggcgaattgt tagtggaatg gctggaagag ctgcaagacg tgacgccgtt
    11821 tatcgatttt ggcccacgta ttggcgatat cccggatgca ttactggcgc ggatcatggc
    11881 ctgtcgccct ttagtgtcac tcaatcgtca agaggctgag attgccgccg aacgttttgc
    11941 tttatccgca gagataacaa cacttggcaa gcaatggcag gagaaatttg ccgcgccgtt
    12001 gatcgttcgt ctcgataaag aaggcgcatg gtatttcagc aacgacgctt ctggctgcat
    12061 tccggcattt ccaacgcaag ttgtcgacac cattggggcg ggcgacagtc atgccggtgg
    12121 cgtacttgcc gggctggcct ctggtctgcc actggcggat gccgtattac tgggcaatgc
    12181 agtggcgtcg tgggttgtcg ggcatcgggg cggtgattgt gcgccaacgc gcgaagaact
    12241 actcctcgca cacaaaaacg tataaatcgc tgcgacagtg gctgatgctg tactcaatag
    12301 gccgttgctg ttggtcaagc gccacttgct tgatcaccag caccggtatt ttgctatcta
    12361 actgaatatg tgactgaaac tcggcatctg gcatccgggc gctaacgcgg gaacgtgtac
    12421 gttgtgggta aatgtgctgg ctgcggaaat agtcataaag agaaattccg atggcatcaa
    12481 catcatgaat taaatgcgcc ggaacccacg attcctcaat cgataccgct tcttcatcga
    12541 catagcgaat acgcttaagc aaaaagacat cacttcccgc ttcgaccgcc agctgctgcg
    12601 cgacttcttc gggacatttc acgacacgct tgttaaccca tagcgtatcg ggctttttcc
    12661 cgcgcaatac cacctgctga gaaaaacctc gcgcttcttt caacgagtat tcgaagatgt
    12721 tgttgatctg cgtgccgtaa ccgcgcgaac gcgtcaccac gccctcttct tccagcgcct
    12781 gcatcgcctt gcgcaccgta atgcgtgaaa cgccggttaa ctgacttaaa tcacgctcac
    12841 cgggcaaaat attgccatgc tccagcaccc cgctgcgcac ggcatttttt accgtttcgg
    12901 caaacttaat ataaagcggc gtgttatccg ccgctaaaat acgctcgtta agttgggcaa
    12961 tcagctgggt atgcgcttgt tccatttgtc tttttcctgc cagggagtct ggtgggagta
    13021 tatcactgct tcttatttaa ggcgtttctt taatcccatc aacgaatgcc tgtaactcct
    13081 ccaccgtgcc attaaacaca ttaaaatcca ccggaccatt gatgccatct acctgtccac
    13141 ggtcggaatg ctgccagaag cgccaggcca tgccgtcatt gtccggacga cgttgatagt
    13201 agtgagccac ccaccacgga tattcattga aatagcccgc cagattcgtg tgataaaaaa
    13261 cggctcctga gtaaataatc ggctttttcc ccgtactttt ttcgaccatt tttagccact
    13321 gacttaccct cttgcgtaat tcttttgccg ataattttcc ccgttcctct acgtccagca
    13381 cggcagggaa atcgccttgt gagaaatcca ccgtttgcag aaataatcgc gcctgaacag
    13441 gcgctgctac cgacggggag aaataatgat acgccccgcg cagcaggcca ttttcgcggc
    13501 ttagttgcca gttacgcgaa aaataggggt ccaccagctt ttcgccttcc gtcgccttaa
    13561 taaaagcaaa ctgtaagcgg ataccgttgt cgcgcatttt tgccacccgc tgccagtcga
    13621 tccgctcctg ccagcgcgaa acatctattc cgtgaatggt gtaactggca ggaatgcgaa
    13681 tagcgaaaga tttaaccggg cgataaccgt agaagtggat gtagtcttta atctgaactg
    13741 ctgtcgaata gaaaaaattc accgtctgac gtggacaaat tgcaacgatg gaaattagcc
    13801 caagagcgca taatagagcg gtgagttttt ttcgactggt aattcttagc tgcattggtt
    13861 tcatccctga atgtcagtgc caaaggctga caataaccaa agcagctata gtacggtgct
    13921 caccaccagg cgtggaagtg gtgaaccgga ccaataccgt gaccaacttc cagcgtgtcg
    13981 gcctgggcta acgccgatga aagccagctt tttgcctcct gtacggtgtc agcccagttt
    14041 gtatggcgcg ggcgtagtgc agccaacgcc gcagagagcg tacaaccagt gccgtgggta
    14101 tttttggtca taatgcgcgg tgcggtaaac cgttgttcac cctcgcgggt aaacagccag
    14161 tccgggcttt gctcatcatc cagatgacca cctttcatta gcactgcgcc acagcccatc
    14221 gccaacagcg atcgcccttg ttccagcatt tcctgttcgg tgcgcgcgtg tggcgcgtcg
    14281 agcaaggcgg ctgcttcggg caagtttggc gttattaatg aaacctgtgg caataatcga
    14341 ctgcgtagcg tggcaactgc cgaaggtgaa agcagtgggt cgccgctttt tgccagcata
    14401 acggtgtcga gtaccacgtt ttggatctga taacgttgca accgttctgc caccgcttca
    14461 acaatatcgg tttccgccaa cataccgatt ttagtggtat cgattcgcac atcgctgaac
    14521 accgaatcga gctgggcggc gacaaaatca ggctcaatgc gatacaccga ctgtacgcca
    14581 cgggtatttt gcgccaccag tgcagtaata actgagcaac cataagcgcc aagtgccgag
    14641 aaggttttaa gatcggcctg aatccccgca ccaccactcg gatcagtacc ggcaatcgtc
    14701 agagcgttaa ttcgtttcat gcctgcacct cctgcgtcaa ttgccagagc gcatcaagga
    14761 aatgtggaac aaaactgcct ggcccctcgc ttctggcgac tgcgcgttct ccggcttgtt
    14821 tcatccagtg acaggcagat gcgacatttt ccagcatatc gcctggtaac gcacaacagg
    14881 cagcgacaac cgccgataat gcacagccag ttcctaccac tttggtcatt atcggatcgc
    14941 caccgtgaat gccaatgata cgatgtccat cggtaacata atccacctcg ccagtgacca
    15001 cgacgattgc gccagtttcc cgtgccagtg tttgtgcagc gggtatcgca ttagctgccg
    15061 cgtcagtggt atccactccc cgtccgccag tagcaacgcc agctaatgcc atgatttccg
    15121 aagcattacc acgtatcgct gccggtttaa aagataaaag ttcatgacaa aaatggcgac
    15181 gataatcgag cgcaccaacc gctactggat caagcgtcca gggggtttga gagctttttg
    15241 cttgctcaac ggcggcacgc atcgcctgtg cgcgtggctg cgtcagcgtg ccaacgttaa
    15301 tcaacaaggc actggcgata gccgcaaact gactggcctc ttcggtttcg ataaccatcg
    15361 ctggcgatgc accgagcgcc agtaaggtat tggcagtaaa ggtttgcacc acatcgttgg
    15421 tcatgcagtg cacaagaggg gaatgttggt gaaaaaggtg taacgtgtgc gcagattgcg
    15481 cggaactcag caggtcgact tgcatagttt gctcctgcca taacgtgaag aagcaatgac
    15541 ccggtggtcc gtgacttccc tacgctggca ttatccagat caggtgatac gggtatttct
    15601 cagccttcac gcagaagggc accccgagtc gtttggttgc gatgataaat cgcagaggaa
    15661 gatggtaatg tccagcgcac gcgttgtaaa cgaggaaaaa ccgcagtttt acaatcgcgt
    15721 taataaatta tttgatataa gaatccagca ccttcagaac gacatccaga tcttcttcac
    15781 gctttagttc atcgccctgg tgaacaatgt gttctgtcag atgaccttta atcacttccc
    15841 gcatcaggcc gtttaccgcg ccacggatag cagcaatctg ttgtaaaact gcagcgcatt
    15901 cgtgcggctc gtcgagcatt ttcttgagcg ccacgacctg gccctgaatc ttactggcac
    15961 gcgctttcag tttctgttta tcacggattg tatgagacat ggcaacacct ggttaacaag
    16021 aatatgaaaa atcatagcac tattaatcta ctggggggta gtatcaggta ctggggggga
    16081 gtagaataag attgccgaat taatactaag aattattatc atgaccgaat ttaccactct
    16141 tcttcagcaa ggaaacgcct ggttcttcat ccccagcgcc atcttacttg gtgcgcttca
    16201 tggcctggaa ccagggcact caaaaacgat gatggcggcg tttatcatcg ccatcaaagg
    16261 caccattaaa caggcggtaa tgctcggact ggcagcaacc atttcgcata ccgcagtggt
    16321 ctggttgatt gcctttggcg ggatggtgat cagcaagcgc tttactgctc aatcagcaga
    16381 accgtggctc cagctgattt ccgcagtgat cattattggc accgcgttct ggatgttctg
    16441 gcgtacctgg cgcggcgaac gcaactggct ggagaatatg cacgagcatg atcatgaaca
    16501 tcatcaccac gatcacgaag atcaccacga ccatggacat catcaccatc acgaacatgg
    16561 cgagtatcag gatgcccatg cacgagccca tgccaatgac attaaacgac gctttgatgg
    16621 tagagaggtc accaactggc aaattttgtt atttggctta accggtggcc ttatcccctg
    16681 cccggcagca attaccgtgc tgttgatttg cattcagttg aaagccctga cactgggcgc
    16741 aacactggtc gtcagtttca gcattggcct ggcgttaacg ctggtcaccg taggcgttgg
    16801 cgcagcaatc agcgttcagc aggtcgcaaa acgctggagc ggatttaaca ctctcgctaa
    16861 acgcgccccc tatttttcca gtctgttgat tggcttagtc ggtgtgtata tgggcgtaca
    16921 tggcttcatg ggcataatgc gataactcaa tcctttaggc ttgcatgtta tcttcagccc
    16981 aggatgattc ttaaatcagc tatttccgct gactctctgc tcgataagga tgcttttagg
    17041 gcatcctttc atttacactt tttacgaaat catgggatca ctaacaaaat atcgcttgtc
    17101 agttatattg tatggcatga aagatatgcg actgatatta cagatcccca aagtggagaa
    17161 tttatgacca ttaaatataa gatgttgctg ggtgcgcttt tgctggttac cagtgccgcc
    17221 tgggccgcac cagccaccgc gggttcgacc aatacctcgg gaatttctaa gtatgagtta
    17281 agcagtttca ttgctgactt taagcatttc aaaccagggg ataccgtacc agaaatgtac
    17341 cgtaccgatg agtacaacat taagcagtgg cagttgcgta acctgcccgc acctgacgcc
    17401 ggaacccact ggacctatat gggcggtgca tatgtgctga ttaataatac cgacggaaaa
    17461 atcattaaag cctacgacgg tgagattttt tatcatcgct aaaaaaagcc ccctcggaga
    17521 gagggggaaa tgcagacacc ttattatttc ttattattag ccacttgctc gtcttgcttg
    17581 ttattaatcg tatttcacgt tgattaatgc ggttgcttcc agtgcgccag atttaacttt
    17641 tttttcatca tatacatagt aactggccat gactggtata ttaataatgt tgctatttaa
    17701 tgaaactcct ggtttatctg ttttccataa tgatgtcgcc gcaccctgat cgccgttggc
    17761 cgaaatttta atggcagcct cagtcccttt tggtggttct gttagatcca acacaaaccc
    17821 tacaccatta tctgttttac cacgcaaaat agtattactt cctttaacta agtcactgct
    17881 agaaagatat acaaccatat tccgcgatgc ttgttttttg tcgccgaaaa gattatcaca
    17941 tttcaacgta attgttcctt ccttactgtt cgccgcaatc tttccctgat ttctgagctg
    18001 gaacaaggcg atatcatcta cttttataac tgtgttttca ggcttacagg tcgtattttt
    18061 aggataatag gaaatactta acccaagcgt aaatgtatcc cccgccagtt tgctggtgag
    18121 ataattcaca caccagttaa acttcaatgc cttacaaata cccaaccaaa aatcgatgga
    18181 tgacgatgac tcaccgccaa tatttgcaat cacggtatct gaaataaacc aggtagattt
    18241 tctttctcca gtaaaatcag gtgtgcctct ggctatttta accacatagg gtaatactgc
    18301 ctgcgcattg ctggatttcc caattgtgtc gctggtttta cctaaagact ctattttaac
    18361 ctttaatttt tcgccattac caaaaggccc aacttcaacc caatcacttt ggatattttt
    18421 ataaactatt ttatccgtgc tcgtatcaca agatatccca gtaaacacga cacttttact
    18481 aaaggaagtt tcattcgcta aaatctcccc aacattaata ccatctgtaa tatttaatga
    18541 tgcagtagta cgacacccag cccagg
//
LOCUS       DAZUCE010000090        18330 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZUCE010000090 DAZUCE010000000
VERSION     DAZUCE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.090"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(18..266)
                     /locus_tag="ACHY03_002605"
     CDS             complement(18..266)
                     /locus_tag="ACHY03_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3905818.1"
                     /translation="MVDHNWHPADIIAGLRKKGTSLAEVSRKAGLSSSTLANALSRPW
                     PRGEKLIADALEIAPEIIWPSRYFDSKGNRLERFMRKR"
     gene            386..652
                     /locus_tag="ACHY03_002606"
     CDS             386..652
                     /locus_tag="ACHY03_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013316693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3905819.1"
                     /translation="MLPYRLKTARLRAALTQEKLGVLAGIEEETARSRVSQYEGGIHR
                     PTFEMMCAFAKVLDVPECYFYTVNDDFAEMILALYLTHYHYSKK"
     gene            748..1017
                     /locus_tag="ACHY03_002607"
     CDS             748..1017
                     /locus_tag="ACHY03_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3905820.1"
                     /translation="MLPVRLKTARLSANLTQEKLGVLAGIEEATARSRISQYESGIHR
                     PTFEMMCAFAKVLNVPECYFYTVDDDFAQAVLDLHTQWAKGSTGR"
     gene            complement(1081..1389)
                     /locus_tag="ACHY03_002608"
     CDS             complement(1081..1389)
                     /locus_tag="ACHY03_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HFS3905821.1"
                     /translation="MAKERLYDDVMVEALREDPAFAQAYLHQAFLDMDEDGGQEAFLL
                     ALRHVVEARGGMATVAQKAGVSRESLYRTLSPTGNPTLKTLRSVVHAAGFQFSALASA
                     "
     gene            complement(1393..1710)
                     /locus_tag="ACHY03_002609"
     CDS             complement(1393..1710)
                     /locus_tag="ACHY03_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3905822.1"
                     /translation="MKTIKTYITADGRDLYAEFFKGLRDPIAKAKIASRINRMASDNF
                     GDHKPCREGVWELRIDQGVGYRVYYSLVEGEVVLLILAGDKRTQDADIDEAIRCLKDY
                     LKR"
     gene            complement(1832..3094)
                     /locus_tag="ACHY03_002610"
     CDS             complement(1832..3094)
                     /locus_tag="ACHY03_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3905823.1"
                     /translation="MALTDTAIRKTKPSNKPFKMTDSSGLYLLIKPNGSKLWYMKYRI
                     DGKEKKLAFGPYPEISLLQARRLRETARNNIRAGVDPAAVKQTEKQKKKNGQTFSQIA
                     TNWHGEHKRWSEHYAKTVMRRLEMYVFPDIGDRLIEEIETEDLLVTLRKVESKGFLEI
                     TARLKNYVTDIMHYAMKKQLLKANPALYLDGEFAAPETNHYPALSLDKLPELLSRTEN
                     YCGRLLTKYTLKLSLMFFVRSSELRFARWSEIDWQQKLWVIPEEREQIENVRFSYRGS
                     KMKIQHIVPLSDQAIAILKQIEALSGHLAFIFPGEYDQDKCMSDNTVNKALRVMGYDT
                     KKDVCGHGFRAMACSALSESGLWSKEAIEKQMSHQERNSVRAAYIHKAEYLEERIEMM
                     QWWSDYLDANRDGYVSAYQYARRLKQAS"
     gene            complement(3261..3336)
                     /locus_tag="ACHY03_002611"
     tRNA            complement(3261..3336)
                     /locus_tag="ACHY03_002611"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3301..3303),aa:Asn,seq:gtt)
     gene            complement(3437..4924)
                     /gene="yeeO"
                     /locus_tag="ACHY03_002612"
     CDS             complement(3437..4924)
                     /gene="yeeO"
                     /locus_tag="ACHY03_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFS3905824.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5085..5160
                     /locus_tag="ACHY03_002613"
     tRNA            5085..5160
                     /locus_tag="ACHY03_002613"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5118..5120,aa:Asn,seq:gtt)
     gene            complement(5198..6148)
                     /gene="cbl"
                     /locus_tag="ACHY03_002614"
     CDS             complement(5198..6148)
                     /gene="cbl"
                     /locus_tag="ACHY03_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFS3905825.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6250..7167)
                     /gene="nac"
                     /locus_tag="ACHY03_002615"
     CDS             complement(6250..7167)
                     /gene="nac"
                     /locus_tag="ACHY03_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFS3905826.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7493..7568
                     /locus_tag="ACHY03_002616"
     tRNA            7493..7568
                     /locus_tag="ACHY03_002616"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7526..7528,aa:Asn,seq:gtt)
     gene            complement(7624..8559)
                     /gene="ldtA"
                     /locus_tag="ACHY03_002617"
     CDS             complement(7624..8559)
                     /gene="ldtA"
                     /locus_tag="ACHY03_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS3905827.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGVDVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(8621..9700)
                     /gene="cobT"
                     /locus_tag="ACHY03_002618"
     CDS             complement(8621..9700)
                     /gene="cobT"
                     /locus_tag="ACHY03_002618"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFS3905828.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLFDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9712..10455)
                     /gene="cobS"
                     /locus_tag="ACHY03_002619"
     CDS             complement(9712..10455)
                     /gene="cobS"
                     /locus_tag="ACHY03_002619"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFS3905829.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVSLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10452..10997)
                     /gene="cobU"
                     /locus_tag="ACHY03_002620"
     CDS             complement(10452..10997)
                     /gene="cobU"
                     /locus_tag="ACHY03_002620"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFS3905830.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(11131..11490)
                     /locus_tag="ACHY03_002621"
     CDS             complement(11131..11490)
                     /locus_tag="ACHY03_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFS3905831.1"
                     /translation="MHCRGCGFIREDWQQDFQPFDGGPVQSLSDAYSDYSVLLLIAAE
                     SEISVPEWLAHSEGTTVILIAEHNARLLTGPAGGAVALIHYLNEICHSRETAVNDAFC
                     RTLTRGSLAIPQCHLLS"
     gene            11469..11765
                     /locus_tag="ACHY03_002622"
                     /pseudo
     CDS             11469..11765
                     /locus_tag="ACHY03_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
     gene            complement(11866..12195)
                     /gene="tmaR"
                     /locus_tag="ACHY03_002623"
     CDS             complement(11866..12195)
                     /gene="tmaR"
                     /locus_tag="ACHY03_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFS3905832.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(12367..13425)
                     /gene="yeeA"
                     /locus_tag="ACHY03_002624"
     CDS             complement(12367..13425)
                     /gene="yeeA"
                     /locus_tag="ACHY03_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFS3905833.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(13623..14096)
                     /gene="sbmC"
                     /locus_tag="ACHY03_002625"
     CDS             complement(13623..14096)
                     /gene="sbmC"
                     /locus_tag="ACHY03_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFS3905834.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAIYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(14215..15381)
                     /gene="dacD"
                     /locus_tag="ACHY03_002626"
     CDS             complement(14215..15381)
                     /gene="dacD"
                     /locus_tag="ACHY03_002626"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFS3905835.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNIDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTDQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAYWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            15590..17017
                     /gene="sbcB"
                     /locus_tag="ACHY03_002627"
     CDS             15590..17017
                     /gene="sbcB"
                     /locus_tag="ACHY03_002627"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFS3905836.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTSQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(17060..17287)
                     /gene="tsuB"
                     /locus_tag="ACHY03_002628"
     CDS             complement(17060..17287)
                     /gene="tsuB"
                     /locus_tag="ACHY03_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFS3905837.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(17301..>18330)
                     /gene="tsuA"
                     /locus_tag="ACHY03_002629"
     CDS             complement(17301..>18330)
                     /gene="tsuA"
                     /locus_tag="ACHY03_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFS3905838.1"
                     /translation="CGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISVQSV
                     GVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLIGSWIA
                     LFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVTVLLVITLWVVMK
                     ELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLG
                     ITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTVRSG
                     LGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKA
                     RLATAAAN"
BASE COUNT         4877 a         4866 c         4116 g         4471 t
ORIGIN      
        1 attttgctgt atcaggatca tcgctttcgc atgaaccttt ccaggcgatt acctttgctg
       61 tcgaagtatc tggaaggcca gatgatctct ggagctatct ccagcgcatc agcaataagt
      121 ttttctccgc gcggccacgg acgggaaagt gcatttgcca gtgtcgaaga gctcaaccct
      181 gctttacgcg atacctcagc cagtgaggtc cctttctttc ttaatccggc aatgatatct
      241 gccggatgcc agttatgatc aaccatcttc atcaccctta ttttgttgtt tcagataaat
      301 atcatttgtt cagcatatct aaacaactgc gatgttaagt aatgctaaac agtaagtcaa
      361 tgtatgatca ttgatggcgt aacagatgct tccttaccga cttaaaactg cccggttacg
      421 agccgctctg actcaggaga aattaggagt gcttgcgggt attgaagagg aaacggcgcg
      481 ctcccgcgtg tctcagtatg agggcgggat acaccggcca acatttgaaa tgatgtgtgc
      541 gtttgcaaag gtattggacg taccggagtg ttatttttat acagttaatg atgattttgc
      601 tgaaatgatt ctggcgcttt atttaaccca ctatcattat tcaaaaaagt aaaaagacgt
      661 tattagccct ttgttcagaa agtctaagca actctcctgt aaagcaatgc taaacatgct
      721 gtcaatatcc ttattggtgg tatagctatg ttgcctgttc gtcttaaaac cgctcgttta
      781 agtgctaatc tcactcagga aaagttaggt gtactggcag gtattgaaga agctacggcc
      841 cgttctcgta tttctcagta tgaaagtgga atacaccgcc cgacattcga aatgatgtgt
      901 gcattcgcca aagtactaaa cgtaccagaa tgctatttct atactgtcga tgatgatttt
      961 gcgcaggcgg tgctggatct acatacccag tgggcaaaag gttctactgg aaggtagatc
     1021 gcagaaaata aaaaaccccg ctggtgaggc agggttcctt aacaacaaat gtaaatccgc
     1081 tcaggcactt gccagcgcag agaactgaaa accggctgcg tggacaacac tacggagtgt
     1141 tttcagtgtc gggttgccag tcggggagag cgtacggtac agcgattcac gcgatacccc
     1201 ggctttctgg gcaaccgtcg ccattcctcc tcgcgcttca acaacatggc gtaacgcgag
     1261 taaaaaagcc tcctgaccgc catcttcatc catatcaaga aatgcctgat gcagataggc
     1321 ctgagcaaag gccggatctt cccggagtgc ttccaccatg acatcatcat ataaacgttc
     1381 tttcgccata catcacctct tcaaatagtc tttgaggcag cgaatagcct catctatatc
     1441 tgcatcctgc gtgcgcttgt cgccagccag aatcagcagg acgacttctc cctccaccag
     1501 gctgtaataa acccgatacc caaccccttg atctatcctg agttcccaga caccttcccg
     1561 gcagggcttg tgatcgccaa aattgtccga agccatccgg ttgattcggg aagcgatttt
     1621 tgccttcgct atggggtcgc gtaacccttt aaaaaactct gcatacagat cccgcccgtc
     1681 agcagttatg taggttttta ttgttttcat aatatgattt tagcttaaaa gctacttttg
     1741 tccaatgagt ttagtttaaa agctacagtc agcatcgaca ttcgaaatgc aaaaaagggg
     1801 aataccctcc ccccttaaat gccctgttag atcatgatgc ctgtttcagg cggcgggcat
     1861 actgataagc cgaaacatac ccgtcacggt ttgcatcaag ataatcagac caccactgca
     1921 tcatctcgat ccgctcctcc agatattcag ccttgtggat gtaggcagcg cgaacactgt
     1981 ttctttcctg gtgactcatc tgcttttcaa tcgcttcttt gctccacaac cctgactcac
     2041 tgagcgcact acacgccatc gcccggaagc catgcccgca gacatccttt ttggtatcgt
     2101 agcccatcac ccgcagcgcc ttattcacgg tgttatcgct catgcactta tcctggtcgt
     2161 actcgccggg aaagatgaag gccagatgcc cggagagggc ttcgatctgt ttgaggatcg
     2221 caattgcctg atccgacagc ggtacaatat gctgaatttt cattttcgaa ccgcgataag
     2281 agaagcgcac gttttcgatc tgctcgcgtt cttccgggat cacccacagc ttttgctgcc
     2341 agtcgatttc actccagcgg gcaaagcgta gctcgctgga acgcacaaaa aacatcagcg
     2401 acaatttcag ggtatatttc gtcagcagcc tgccgcagta attctctgtc cgggataaaa
     2461 gctcaggcaa tttatccagc gaaagggccg ggtagtgatt cgtctctggc gcggcaaact
     2521 caccatccag atacagcgcc gggttcgcct tgagcaactg ctttttcatc gcgtagtgca
     2581 tgatatcggt gacgtagttt ttcagccgcg cggtgatctc cagaaagccc ttgctctcca
     2641 ccttgcgcag agtgaccagc aaatcctccg tttcgatttc ctcgataagg cgatccccga
     2701 tatccgggaa aacatacatt tccagcctgc gcataacggt tttcgcgtaa tgctcagacc
     2761 agcgtttatg ctcgccatgc cagttagtgg cgatttggct gaacgtctgt ccgtttttct
     2821 tcttctgctt ctcggtttgc ttcacagcag ctggatcgac accagcccgg atgttgttgc
     2881 gtgccgtttc ccgtaagcgg cgggcctgca acagcgagat ctccgggtag gggccaaagg
     2941 ccagcttttt ctctttgccg tcgatgcggt acttcatgta ccagagtttt gatccgttag
     3001 gtttgatcaa caggtacagt ccggaactgt ccgtcatttt gaaaggtttg ttggagggtt
     3061 tagtcttgcg aatagcggtg tctgttaatg ccatagcgtg ggggctccgt ttgctcaccg
     3121 aaccgtgagg cccctgatta ggcccccaaa aactatggat gtcaagaaac ccggcagcct
     3181 cttgcgagac aaccgggtaa agataagttc ttgtttttta actattttga gacacataga
     3241 gatgtctcaa aactaaaatc tggctcctct gactggactc gaaccagtga catacggatt
     3301 aacagtccgc cgttctaccg actgaactac agaggaatcg tgagaacgag gcgaatatta
     3361 gcgatgccca cccacaatgt caaagcctgt tttttaaatt tgaaatcgtt tgctgaaata
     3421 atctgcattt tgtcgtttat tccgacacaa ctggcttttt ttcacacttt tgcggctcgg
     3481 atcgagggta tttccatagc caacgtccag taaccattcg ccagtaaaac agcacggccc
     3541 gcacagccca gtcggcaaac attcccatcc agacaccaac cacaccccag ccaagcatga
     3601 tgcccagcac ataaccgacg acaacccggc aaccccacat gctcaacata gaaacccaca
     3661 tagcgtaacg ggcgtcacga gcccccttaa atccagcggg tagcacccat gaggcggacc
     3721 aaataggcat aaataaagca tttagccaaa tcagaatcac aacgacatgt ttaacctgtg
     3781 gatcctgggt gtaaaacgat gccataaccc cggcaaaggg agccgttagc caggcgatgg
     3841 ccgttaatcc aagagtagac agccagaaca catgccgcaa ctgaatctct gcttgagcta
     3901 tctgccctac tcccaacctt cggcctgtaa tgatcgtaga agcagagccg agcgcacttc
     3961 cgggtaagtt gataagagcc gcaattgaaa acgcgataaa atttccggca ataacactgg
     4021 ttcccatccc ggcaacgaac atttgggtta ataaccgacc actggtaaat aatactgatt
     4081 cgacacttgc aggaatacca atccccatga cttcccagat aatgctaaaa ttcagtggct
     4141 taaaatagct ctttaacgaa atccttagcg caggattaaa accaatcgcc aacacccaca
     4201 aaattgcaac tgcgccaata taacgagaaa tggttaaacc caacccagcc ccgacaaatc
     4261 ccagtcccgg ccaggagaaa aggccgtaaa tcaatatgcc gctaataata atattaagaa
     4321 tattcaggct accgttaatc agtagcggta ttttcgtatt ccctgcacca cgaagtgccc
     4381 cgctaccaat tagagtgatg gcagctgctg gataactgag taccgtcagc tccagataag
     4441 tcaacgccag tgctttaact tctgtcgtgg catcacccgc gacgaaatca ataatttgct
     4501 cgccaaaatg atgaataagc gttgccaaca gtacggcaaa caacgtcatg atcaccaatg
     4561 actgccgcgt cgccaccctc gctcgtcgtc gatcccgctt accgagacta aatgccacaa
     4621 cgacagtagt accaagatcg atagcagcaa aaaaagccat aatgaccata ttgaagctgt
     4681 ccgccaatcc cacgccggcc atcgcatctt ttcccagcca gctgaccaga aaagtgctca
     4741 gaacccccat caacaggaca caggcattct ccatgaagat aggaacagca agcggggtta
     4801 tctcgcgcca gaacaacact ttgtagctct tgcgtttagc gtgccagcga gtgccgtgaa
     4861 caacctggcg taaagcagag gagatattca aagccgacct taattgcaga aagtgaaacc
     4921 acatttcaaa taatgaggga gaatcagcaa agctgcaaag attttcgcca acaaattgtc
     4981 tgcaaatgca acaaactgtt gatagaaacg gcaaacagtt ggggaattta aaaatcgggt
     5041 ttgacaaaag atttttcgcc gttaagatgt gcctcaacag cgattcctct gtagttcagt
     5101 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     5161 aatttctgaa aagcccgctt ttatagcggg ctttctgcta tatctgataa tcaatttcct
     5221 cttcactgtt ttccatcacc tgccgcttga tatcctcaac tgaaagtcct gcattacaaa
     5281 gttccagaaa gcgccagaca tagttacgct gaagttgtcc tcgcttcagt cccaaccaga
     5341 cagtattagc atcaaaaaga tgccgcgtat ccaggcggat taaattcttt tcctcttgtt
     5401 caccgctgga ttgttcggca actattccga tcccaagccc aagagcaaca taggttttaa
     5461 tgacatcaga atcctgcgca cttaatacaa tatctgccag caaacctttg cgggcaaatg
     5521 cgtcatcaat acgtgagcgc cccgtaatcc cctgtcggta agtgattaac ggccacttcg
     5581 ctattgattc cagcgtcaat ggtgtaattt gcgtcaaggg atgatcgagt ggaacaagca
     5641 aactatggtg ccaacgaaac cacgggaagg cgacgagctg cgggtcatta ctcaaacgct
     5701 cgctggcgat accaatatca gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg
     5761 tcccctggat tagctcgagc cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct
     5821 ctggcaagct ataacgtgcc tgagtatgcg tcgttgcaat agtgagaacg ccagacgtat
     5881 cgttggtaaa caggtctgca agccgacgaa cattactggc ttcattgaga atacgttctg
     5941 caatgaccag taatgctttg cccggttcag tcatgcccag cagtcgctta cctcgtcgaa
     6001 caaatatttc gatgccaagt tcatcctcca gttcccgaat atgacggctg acgcctgact
     6061 gtgaggtaaa aagcatattc gcaacctctg tcaggttgta atcctgacgt gcagcttcgc
     6121 ggattatctt tagttgttgg aaattcacgg taaactccgg gcagttcaga tatcccgtta
     6181 ttgttaaagt ctaacgcccg gcataacaaa taataaaaac ccgcatctta ttccatcccg
     6241 atataacgct tagctcacca attgccactg ccttttttcc atcactggag aactaatcac
     6301 tgacattaac aactctttca ctgcctgtgc ctgtggcgat aagttcgctc tggcgggtaa
     6361 atttaatgac aaagagagac tcatggaagg cgtggtaatg cgtgacatcc acccatttac
     6421 tgcgccacat aacgaacgcg cggccgattc gggtaatact gcaacgccca tgccgctggc
     6481 aatcgctgcg gtaagcgtgg caatagactc aatttcacca atgacttttg ccgtgagtcg
     6541 ccgtagggaa aacgcctcat caacacgaag tctaacagcg ctgtaatcac tggggagaaa
     6601 gaggttcatt tgtgcaatag cattaacatc aacgctttgc cccgggcaat cttgagttcc
     6661 taccaggaaa agatcttctt tcagcaaagc ctgactggat accccagcca caggggaatg
     6721 ctcataaatc accgccatat cgagttggtg atttatcaat ttttcgttaa gcactgcacc
     6781 gctattttca tgaagataga taacgatctc cgggaattca gcgcgaaccg cctgtaataa
     6841 gggcatggtg atagatgacg cagcggttcc tggcgcaaag ccaatcgaga cttgccccga
     6901 taatgcctga ccaacgttat gcaccgccag ttgggcctgc tcacactgac gtaaaatggc
     6961 ccgcgcatgg gtatagagaa tttttccggc gtctgttggc gtaacgcccc gttttgtgcg
     7021 aatcaaaagt tgttgattta actcaccttc gagtgtggca acctgctggc tgagcgctgg
     7081 ttgtgcgata tgcaatactt cagcagcctg agtcaggcta ccaatatcta caatttttac
     7141 gaagtatttc aggcgtctga agttcatgtt gcctccggtt tttaagaatc ggcccaagtg
     7201 ccgccattac ttacaaccag attgcaagat gcttgccagt tttattttgg cgttgatgta
     7261 caagctaacc aactgtcaaa taagagatta tgatagattc gtcattggct cttttcacca
     7321 gcagtcgcgt tcccctgccc tataaaagga gggtatgcac cacgatggtt cattaccaat
     7381 aagattgaaa tctcatcact ttgttgaaat tggcagcaaa caaacaaaaa aatgcatttc
     7441 accctttgac atcaccatcc actgccatta atatgcgccc cgttcacacg attcctctgt
     7501 agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag
     7561 aggagccaaa ttcaaaaaag cctgctttct agcaggcttt ttgctttcta attaccaact
     7621 ctcttaaaac atctgtcttg aaccagaact aatttgcaca ggcattcccg atcgacgttg
     7681 caacgcagca tttgcgcgat tcacatcaac ttcttgcccg ttgataaacg cccgcaaaga
     7741 tggggttacc ggcaatggca cttttcggtc agactcatat tctgcacgat tgcgcgacaa
     7801 cggctcatga acttccagcc agttcgagcc atctggttca gtggtgtatt ttactggctg
     7861 gtcgataatt tgcacacgcg tcccaacagg aacattatca aacagatatt tgatatcgtc
     7921 attgcgcaga cgaatacagc cctgacttac ccgaagtcca ataccaaaat tggcattggt
     7981 accgtggatg gcatacaacc tgccaatata aatcgcgtac agccccatgg gattatcggg
     8041 ccccgcagga acaaatgcgg gcaaactctc ccctcgtttc gcatattcgc gccgagtgtt
     8101 cggcgttggc gtccaggttg gagcttcttg tttacgttca acggtagtca cccagttacg
     8161 cggggtttct cgcccagcct ggccgatacc aataggaaag acttccacag tattactgtc
     8221 tggcgggtag taataaagac gcatctcagc gacgttaacc acaatccctt tacgaacagt
     8281 ggcgggcaaa atcagttgct gcggaatggt gagttgtgag ccggacttcg gcaagaaaac
     8341 atcaacgccc gggttcgctt ccagcatgtt acttaaccct tgcccgtatt gtgcggcaaa
     8401 agtctccagc ggctgggtat tgtgatcagg aacagttaca gtaaacgact gccccactaa
     8461 acggctaccc tctggaggta atggataagt taccgccagg ctagtatggc tggcaaaaag
     8521 cagagcaaat gagcaaagaa tatttacacg acgcatcatg tccctttcct atgtcgcgaa
     8581 agctatccgt taagtatagc ttttatcaga cttttcgttt ttaactgttc aaatcagaag
     8641 tcgtattccc cggtagaaca atattactgg cagcaagttc gcccatgttg ttatatatcg
     8701 cacaggcagc ttcgatgatg ggcatcgcca gagctgcgcc actcccctca cctaaacgca
     8761 tatccatatt gagataaggc tccagcccca aatgcgacag cgctatacgc gccccttttt
     8821 ctgccgacag gtgagaagga atgagatacg gtttgattgc aggagacatc tggcaggctg
     8881 cgagcgcagc agcataagaa agaaatccat ccagcaacac gggtaaaccg caggaagcag
     8941 cgcctaacat cacgccagcc attccgacca aatcaaatcc acccactttt gccagaacat
     9001 caacgccatc ctgcggattt ggttgattca acgtaatcgc ccggcgcaca acatcaattt
     9061 tattagccag tttatctgtc ggcaggtttg cgccaatccc aaccacttct tcaggatccc
     9121 ggccagtgat tgtgctgacg attgccgctg ccggtgtcgt gtttgccatc cccagttcac
     9181 ctacaccaaa cagcgtgaca ccgttttttg ccagctcccg cgtataacat atgacgtcga
     9241 aaagcaactt ttcagcctga cggcgactca ttgccggagc tgaagcaata ttgccgctac
     9301 ctcgtgcgac acgcatgttg ataagtccgg ggataggctc agcactatca ataccaacat
     9361 caactacgtg gacgttagcg cccgcttgtg ctgccagcac acacacgccg gttgttccgc
     9421 gggtcatatt ttcagcctga atggctgtca cttcttttgg ggaaatagcg accccttcct
     9481 cccagacgcc gtgatcggca cacataacca gtaccgcttt tttgcccaca tgcggtatgc
     9541 cattcaaccc cggcattccc gccagttgta tggcaagcgc ctccagtctt cccaggctac
     9601 caacaggttt gagtaacccg tcaatatgcc gttgtgcacg cgacatagcg gcaggatcga
     9661 tagcagggat cgtattcagt aaatcggcaa gtgtttgcat gtcacgtctc tttatagcag
     9721 tgccagcagg aagaccagtt caccaagctc tatagcagct cccagcgtat cgccggtttg
     9781 cccgcccagc gtacgtttga gaagctgacc gaggatgaaa atcgccacca tcgtcaccac
     9841 catagcagcc acaccatgca tacccggcaa taatacggca gcaaaaatag cggctaagcc
     9901 gagtgtgacg caggtttgtc gcccatcaat tttgccaatg aacacattgc caagcccttc
     9961 ttcccgggca taacgatgac gatacatcaa taatgcggca gtcccgcggc tgaccgcaca
    10021 tgctgccgcc agcgatgcaa ggatcggttc gccacgcaga gccaactcgc ttaacaccag
    10081 aatctttgcc agtacaacaa aaatcaatgc cagaccgccg tgggtgccta aacggctatc
    10141 acgcatgatt tccaacatgc gatcgcggct acgtgcagaa aatacaccat cacaggtatc
    10201 ggcgaggccg tccaggtgga atcccccggt catcagcgcc agcacaagta cgctaaacag
    10261 tgccgccagt gatacgccac accatgcctg cagcaccatg aagaccagcc cgctaatcgc
    10321 gccaagcaat aatccaatta aaggaaaagt aataataccg cgagaataat gctcgaaatc
    10381 cagtccctgg gaccagcgac gcggtacagg cagacgcgta ataaaagaga gcatcgccca
    10441 aaataattta ctcatttgat ttttactcca atacccgaaa ccaccagcca cacttcattt
    10501 gccgcagcgg ccagctgctg atttacccgc ccggcaatat cacgaaaatg tcgtgccaga
    10561 cgactctccg gcacaatccc cattcccact tcgttagtca ctaatacaac ctttgcgggg
    10621 caacgttggc aggcagcaat caacgactga atctcagcat taatcgcctg ttccatcgcc
    10681 tgataatccc attcatcagg gtctttatcg ccgccataat caaacaacag attagtcacc
    10741 attgtggtaa cgcattccag caacacagct tcatgcgggt taatgtctgc atgaattaat
    10801 tcatcaagat gttgccaacg ttccaccgtg tgccagtgcg ccggacggcc ttgccgatga
    10861 tgttctatcc gtgcagccat ctcatcatca aggatttgtg aggtggcgat atacagaacc
    10921 tgtgaagagt ccccaataag cgcctctgca tggcgactct tcccgctccg tgcgcccccc
    10981 gtcaccaaaa tcatcatacc gactcctgat tcaattgtat gatggtattc attgaacaga
    11041 acaatggatc acccaccgtt gctgaccgca aaacatttac gcgattaata ctgcgcgtaa
    11101 tataattaaa aatccagcat tctcaatcca ttacgaaaga agatggcatt gtggaatagc
    11161 cagacttcct cgtgttaacg tccgacaaaa tgcgtcattg acagcggttt ctctggaatg
    11221 acagatttca ttcagataat ggattaatgc tacagcccca ccagccggac cagtcagtaa
    11281 acgggcattg tgttctgcaa tcaaaattac cgtcgttccc tcagaatgag cgagccattc
    11341 tggtacggat atctcgcttt cagctgctat caatagcagc actgaataat cgctatacgc
    11401 atctgataac gattgcacag gcccaccgtc aaaaggctgg aaatcttgct gccagtcctc
    11461 acggataaaa ccgcagcctc tacagtgcac tgttggtcag tgcctgcgaa gcagacatta
    11521 tcgcgctggt gcttaatgcg gacgcaccgt ggtcaccttt ttcgccaggc tttacctcgt
    11581 caatgaatcg cccggtcatt ggagtagtca ccaaagcaga tttagccagc atggaagaaa
    11641 tatctttggt gaaatgttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg
    11701 cggttaataa caatggagtt gcagaacttt tagccttgct gcatacagaa gaaggctgtc
    11761 gctaatttcg acaggttctc agatgagaat gatgacggtg atgcacagaa aagaatcaat
    11821 gagcaatgag ggttgccagg caaccctcat tgaataaaac aggaattact tcgcttcgcc
    11881 gtttttcatt tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag
    11941 ctcggcattt ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc
    12001 gatgatgccc tggattgctt caacgctcat ccccggcttg atgtaatcgc tcaggttgtc
    12061 cagcagcagg acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg
    12121 ttgcagtttg ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg
    12181 cttggtagtt tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc
    12241 gctattcgtt agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt
    12301 ttcactatca taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt
    12361 caggaattat ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc
    12421 cgtttccatg ttcagccaca cataaccata gattggtgtt tccacaacct tcaggtcatg
    12481 gtggttattg agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc
    12541 aaaaaccggc tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg
    12601 catcatgtgc tgggtatcac gaagtttttg cgcgttcaat aacacgaaat ggctggggcg
    12661 cgtggcccag tatgcattga tttgcaactc cagcatacaa accagattgc ggttaatggt
    12721 ctggatacct tcatatatcg attttggaat acgagtttct ttgctggcgg gcgcaatcag
    12781 cccacgcatt ttcacggcat cggtcaggag tttttgtaga tggctttcca gacgtgggcg
    12841 ttcgagtaag ttcggtgaga atgcagattg atagacccga ttatactcgg tcagactttt
    12901 cgccagttga atacgccagt ggatgaacgc ccgttgtggc cagataccgg taaacaacat
    12961 tgccagtaaa gagccgagga tcacatcgcc gcttcgccat aacgccgtat caatttcacc
    13021 tgtaggggaa cccacaacaa ttgccagcgt caccccaatc aataaacctt gatacggttt
    13081 cttgcccagc gccagccaac cgcaaagaaa catagccgcc gcgcaccaga ctaacatcag
    13141 cggtaacgaa attaactcca gttgcagagc gataagacct aaaatcgaac ccaacaccgt
    13201 accgccaata cgttcgaagg cgcgagggac aacgttaccc cagaacgaga ttggccccat
    13261 aatcaccacc atggtgacca gcggccaggt gctttccggg atagtaaaca ggcggataat
    13321 gagaaaagtg agcaggaatg ccagcgcgac ccgagtacca tgcacaatgc ggtaatggcg
    13381 gtatacccgg atttcaaacg ggcttaatga cttatcggca cgcacgcaga aactccgtaa
    13441 tgaagattaa aagcgctcat aactcatctg tcggccagaa ggcgcgcctc tgaattatcc
    13501 gacggacgaa ccgggaacag tgtaatttcc cggcaggagc gaaaaaattg aaaggcgcat
    13561 cttactcttt tcgctttcaa aaaaagagtg gtcatcgcgt taacacaccg ccctgagatg
    13621 aattagtgat gttttggctg caccgcaaca tacatttcga tatcccagta cccatcttcc
    13681 gcgccattgt tcaaataaac ctcgaagcag ggctttggta acatttcata agcactgtcc
    13741 tgcaagaggc tattaaagaa ctgataccag ggtttagcaa aatcatcacc gactacacga
    13801 gctaccgcca ccgcatactg accacctgaa atttctgtca gaatgacgcc ctcactgttt
    13861 tcgggaagcg taaagtaacc cggcaccgtc acgacggtgt cgcagcgtaa tttttcggcg
    13921 ggtgtttcat ctggattgtc gtaatagata gcaacccact ccttcggcac aatattttta
    13981 ctatctaccc acatcatcaa ctgctcaaaa cctttcttta ccgtctgttc ccacgggcca
    14041 acgagatgga aacctgcaac ggtacgtttc tcttcctgct taatctcgta gttcatgacg
    14101 cctccattaa tactgttttt atatacagta tagttgcaaa attaaaacca caaggaatga
    14161 gtgttgatta tgcgagcaga ctcgcactcc tgccagtctg ctgcaaaaga aaggtcaggc
    14221 cttatggtgg aaataatcac tcaggcggga aaacatgccg ccttccccga cagattccag
    14281 ggtaaccagc ggccagtacg ccacctgttt atcacggtcg taaagttcaa tttcccctac
    14341 ccgctgatgg gcgctaattg gcgcggtgag ttctttacca tcaagggtat atttggcttt
    14401 gatatgtgga atttcggctt tcggtagcac catccagaac tcttggtccg ttcccaggtc
    14461 gatattttct ttatcgccat accagatgcg ttccgttccg acctttttcc cacggtgcaa
    14521 aatttgcacc gtagtaaagt tttgttgccc ccaacgcagt aattttcttg cctcttcctc
    14581 acgacctttt gcactgtcag cccccattac cactgcaatg agacgacgct gcccatctac
    14641 agccgaagca atgagattaa acccggcacc agaagtatga cccgttttca ggccgtcaat
    14701 attcatggtt ttatcccaca acaacccgtt acggttttgc tgggtgatgc cgttccaggt
    14761 gagacttttc tcactgtaca tatgataaaa ctcgggctcg ccgtggatga tagcgcgaga
    14821 aagcacagct aaatcataag ccgagctatg ctgacctggc gcatccaggc catgcactgt
    14881 ttcaaaatgc gtatccttga gatgcagctt ctcggcatag ttgttcatca tttcaacaaa
    14941 ctgccgttgc ccaccggcaa tatagtcagc cagtgcaaca caagcgtcat ttccggaatc
    15001 cacaattaaa ccacggctta aatcacgtac cgatacgcga tcgccctctt tcaaaaacat
    15061 cagtgaagaa ccgacaaaca ccggattatc tttcgcccac gcatcgcgcc ccacggtgac
    15121 aatatcgtct ggcgtaatgc gatgactatc gatagcacga tccacgacat agcccgtcat
    15181 cagctttgtc aggctggcgg gattgcgctg ttgatgctca ttacccgcgg tgaggatctg
    15241 accggtggtg taatccatca atacccagga cccggcatga atctcaggag gctgaggtga
    15301 aaaaggaatg ttttccgccg caaaaccaga cgataagtta aaaacgaaca aagaagcagc
    15361 aataataaga cggcgtttca acagcaaacc ctcaggagtt tcaaatagct gttcttttta
    15421 cggaaatact tatgaactgg ctggaataaa gtgcaagaaa atgtgactac cctctcattt
    15481 tcatctgaca tgatctgttg ccactcgctg tcaaattgtc gcgctaaagc tgattagcac
    15541 ggtgatattt gatactctgg cagacagcag aaataacgga tttaacctaa tgatgaatga
    15601 cggtaagcaa caatctacct ttttgtttca cgattacgaa acctttggca cgcaccccgc
    15661 gttagatcgc cctgcacagt tcgcagccat tcgcaccgat agcgaattca atgtcatcgg
    15721 cgaacccgaa gtcttttact gcaagcccgc ggatgactat ttaccccagc ctggagccgt
    15781 attaattacc ggtattaccc cgcaggaagc acgggcgaaa ggagaaaacg aagccgcgtt
    15841 tgccgcccgt attcactcgc tttttaccgt accgaagacc tgtattctgg gctacaacaa
    15901 tgtgcgtttc gacgacgaag tcacacgcaa cattttttat cgtaatttct acgatcctta
    15961 cgcctggagc tggcagcatg ataactcgcg ctgggattta cttgatgtta tgcgtgcctg
    16021 ttatgcgctg cgcccggaag gaataaactg gcctgaaaat gatgacggtc taccgagctt
    16081 tcgccttgag catttaacca aagctaatgg tattgaacat agcaacgccc acgatgcgat
    16141 ggctgatgtg tacgccacta ttgcgatggc aaagctggta aaaacgagtc agccacgcct
    16201 gtttgattat ctctttaccc atcgtaataa acacaaactg atggcgttga ttgatgttcc
    16261 gcagatgaaa cccctggttc acgtttccgg aatgtttggg gcatggcgcg gcaataccag
    16321 ctgggtggca ccgctggcgt ggcatcctga aaatcgcaat gccgtaatta tggtggattt
    16381 ggcaggagac atttcgccat tactggaact ggatagcgac acattgcgcg agcgtttata
    16441 taccgcaaaa accgatcttg gcgataacgc cgccgttccg gttaagctgg tgcatatcaa
    16501 taaatgtccg gtgctggccc aggcgaatac gctacgcccg gaagatgccg accgactggg
    16561 aattaatcgt cagcattgcc tcgataacct gaaaattctg cgtgaaaatc cgcaagtgcg
    16621 cgaaaaagtg gtagcgatat tcgcggaagc cgaaccgttt acgccttcag ataacgtgga
    16681 tgcacagctt tataacggct ttttcagtga cgccgatcgc gcagcaatga aaattgtgct
    16741 ggaaaccgag ccgcgtaatt taccggcact ggatatcact tttgtcgata aacggattga
    16801 aaagctgttg ttcaattatc gggcgcgcaa cttcccgggg acgctggatt atgccgagca
    16861 gcaacgctgg ctggagcacc gccgccaggt attcacaccc gagtttttac aaggctatgc
    16921 agaagagatt cagatgctgg cgcagcagta tgccgacgat aaagaaaaag tggcgctgtt
    16981 aaaagcgctt tggcagtacg cggaagagat tgtctaattt gaagcctccg ccgctgatac
    17041 ggcggaggac aatgttgggt caggcttttt gaacggtgat gctccacgca gcatcgccaa
    17101 tctgctgata atcggtgatg gcatgtcctt cttctgccgc ccactgtggg attgcttcag
    17161 ttgcctgggt gcaatcaaac tcaattacca actcatcgcc gctggccatt tctgccagtg
    17221 ctgcctttgc ttctatgagt ggaaacggac agacctgcgt caccacatcc agctttttaa
    17281 tcgccatctt tacaccttac ttaatttgcc gcagcagttg ccagtcgcgc cttacgctgc
    17341 ggtcgaacat acacaagcca ggacgcagtc cagactccga gaatcataaa tacaaggcca
    17401 atccagccct gccaggtcat cattgcagtc ataaccagtc cattaccgat agagcaacca
    17461 cctgcaatac tggcaccgaa gcccatcagt acaccaccta acccgctacg tactgttgtt
    17521 tgtgcatcag ctgcgcgaac gcggaactca cggctcgctt tggctgcaat aaatgacccc
    17581 acgaagatcc ctaacactag gaaaacgccc cagttaatgt atttcacgtc acccgcgacc
    17641 agaaattgca gaatattggc cgttggggaa gtgattccta acccaaacat gcgtccggtt
    17701 gcttcactca ggggccaggc taacagcgca atcaaaccga tgagtacagc agtgacaaag
    17761 ggatgccagc gtttttcaaa cagaatatga gcgatcccgg ttcggcgcgg cggtaaggtc
    17821 gcgactttca gttttggctt cttcaactct ttcatcacca cccagagcgt tatcaccagc
    17881 aaaacggtaa ccaacggcca cacagacaaa ttaaaagtct cagcaataga gttatgttca
    17941 gtactgtagt gctgcaaggt ttgatttaaa ccactggcat gtggagaacg catcaccgca
    18001 ctcatcacca tataagtgaa aagcgcgatc caactgccga tcaatccctc acccgcgcga
    18061 taccaggtcc cggtggcaca tccacccgcc agaacaattc ccagcccgaa gatataccca
    18121 cctataacag taccaagcca cgggaacgcc ccggcttcgt aagtcagtag gcccgcctga
    18181 attaacgcaa aaacccccac gctttgtacc gaaattgcaa tcagcaaggc gtaaaacatg
    18241 cgattatttt tcgcgatata catatcgcga aaaccacctg tcaggcaaaa acgcccacgc
    18301 tgcatgacaa atcccagcag agcaccacaa
//
LOCUS       DAZUCE010000091        18190 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZUCE010000091 DAZUCE010000000
VERSION     DAZUCE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.091"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            173..1393
                     /locus_tag="ACHY03_002630"
     CDS             173..1393
                     /locus_tag="ACHY03_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFS3905839.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLHELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1366..1995
                     /locus_tag="ACHY03_002631"
     CDS             1366..1995
                     /locus_tag="ACHY03_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFS3905840.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLGGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1996..3156)
                     /gene="ybdL"
                     /locus_tag="ACHY03_002632"
     CDS             complement(1996..3156)
                     /gene="ybdL"
                     /locus_tag="ACHY03_002632"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFS3905841.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3265..4353
                     /gene="hcxA"
                     /locus_tag="ACHY03_002633"
     CDS             3265..4353
                     /gene="hcxA"
                     /locus_tag="ACHY03_002633"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFS3905842.1"
                     /translation="MPHNPIRVVVGPANYYSHPGSFNHLHDFFTDEKLSRAVWIYGER
                     AITAAQTKLPPAFELPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4363..4560)
                     /locus_tag="ACHY03_002634"
     CDS             complement(4363..4560)
                     /locus_tag="ACHY03_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFS3905843.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4713..6818)
                     /gene="cstA"
                     /locus_tag="ACHY03_002635"
     CDS             complement(4713..6818)
                     /gene="cstA"
                     /locus_tag="ACHY03_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFS3905844.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6999..7412)
                     /gene="entH"
                     /locus_tag="ACHY03_002636"
     CDS             complement(6999..7412)
                     /gene="entH"
                     /locus_tag="ACHY03_002636"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFS3905845.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7415..8161)
                     /gene="entA"
                     /locus_tag="ACHY03_002637"
     CDS             complement(7415..8161)
                     /gene="entA"
                     /locus_tag="ACHY03_002637"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFS3905846.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8161..9018)
                     /gene="entB"
                     /locus_tag="ACHY03_002638"
     CDS             complement(8161..9018)
                     /gene="entB"
                     /locus_tag="ACHY03_002638"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFS3905847.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMAQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9032..10642)
                     /gene="entE"
                     /locus_tag="ACHY03_002639"
     CDS             complement(9032..10642)
                     /gene="entE"
                     /locus_tag="ACHY03_002639"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFS3905848.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVHLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLITKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPSEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10652..11827)
                     /gene="entC"
                     /locus_tag="ACHY03_002640"
     CDS             complement(10652..11827)
                     /gene="entC"
                     /locus_tag="ACHY03_002640"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFS3905849.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQLLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12016..12972
                     /gene="fepB"
                     /locus_tag="ACHY03_002641"
     CDS             12016..12972
                     /gene="fepB"
                     /locus_tag="ACHY03_002641"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFS3905850.1"
                     /translation="MRLAPLYRNALLLTGLLLSGISAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12976..14226)
                     /gene="entS"
                     /locus_tag="ACHY03_002642"
     CDS             complement(12976..14226)
                     /gene="entS"
                     /locus_tag="ACHY03_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFS3905851.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14337..15341
                     /gene="fepD"
                     /locus_tag="ACHY03_002643"
     CDS             14337..15341
                     /gene="fepD"
                     /locus_tag="ACHY03_002643"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFS3905852.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15338..16330
                     /gene="fepG"
                     /locus_tag="ACHY03_002644"
     CDS             15338..16330
                     /gene="fepG"
                     /locus_tag="ACHY03_002644"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFS3905853.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16327..17142
                     /gene="fepC"
                     /locus_tag="ACHY03_002645"
     CDS             16327..17142
                     /gene="fepC"
                     /locus_tag="ACHY03_002645"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFS3905854.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17139..>18190)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHY03_002646"
     CDS             complement(17139..>18190)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHY03_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFS3905855.1"
                     /translation="LLNLISVLWRAKKTVMAVVFAFACAGLLISFILPQKWTSAAVVT
                     PPEPVQWQELEKTFTKLRVLDLDIKIDRTEAFNLFIKKFQSVSLLEEYLRSSPYVMDQ
                     LKEAKIDELDLHRAIVALSEKMKAVDDNASKKKDEPSLYTSWTLSFTAPTSEEAQTVL
                     SGYIDYISALVVKESIENVRNKLEIKTQFEKEKLAQDRIKMKNQLDANIQRLNYSLDI
                     ANAAGIKKPVYSNGQAVKDDPDFSISLGADGIERKLEIEKAVTDVAELNGELRNRQYL
                     VEQLTKANINDVNFTPFKYQLSPSLPVKKDGPGKAIIVILSALIGGMVACGSVLLRYA
                     MASRKQDAMMADHLV"
BASE COUNT         4266 a         5159 c         4716 g         4049 t
ORIGIN      
        1 ttgaccgtct aaatgagtgt ttttgatata taacctcaga cagctactat aactttcacc
       61 tatttattca cagcgcgaat gaaaaataaa acactctgct tttcagtgtt tactttataa
      121 ttaacagtct tatctcactg atcaccctgt aacgttcaga gagcgtcttc agatgtctat
      181 ttataaaatt cctcttccgc tcaatattct ggaagctgca cgagagcgta ttacctggac
      241 actcaacacc ctaccccgcg tttgtgtctc attttctggc ggcaaagact ctggtttaat
      301 gctgcatctg acagccgaac ttgcccgaca aatgggcaaa aaaatctgcg ttttgtttat
      361 cgactgggag gcgcaattct cttgcaccat taattatgtt cagtccctgc acgagttgta
      421 caccgatgtc atcgaagagt tttactgggt tgcgctcccg cttacgacgc aaaattccct
      481 ttcacaatac caacccgaat ggcagtgctg ggaacctgat gtcgaatggg tgcgtcagcc
      541 cccgcaagat gcgataaccg atcctgactt tttctccttt taccagccag gcatgacctt
      601 cgaacaattt gtacgtgagt ttgccgaatg gttttcacaa aaacgtccgg cggcgatgat
      661 gatcggcatc cgtgcggatg agtcctacaa ccgttttgtc gccatcgcca gtttaaataa
      721 acaacgtttt gccgacgata aaccctggac tacggccgca ccaggcggtc atagctggta
      781 catttatccc atttacgact ggaaagtggc tgatatctgg acctggtatg caaatcatca
      841 aagtctctgc aacccactgt ataacctaat gtatcaggca ggcgttcctc tgcgtcatat
      901 gcgaatttgc gaaccttttg gcccggagca acgacaagga ttatggctct atcacgttat
      961 cgaaccggat cgctgggctg ctatgtgcgc acgagtcagc ggggtaaaaa gtggcggcat
     1021 ttacgccgga catgacaatc atttctatgg gcatcggaaa atcctcaagc cagaacattt
     1081 agactggcaa gaatatgcat tattgctgct caatagcatg ccggaaaaaa cagctgagca
     1141 ttaccgcaat aaaatcgcca tttatttgca ctggtatcag aaaaaaggca tcgaggttcc
     1201 acaaacccag caaggggaca ttggcgcgaa agatatcccc tcctggcggc ggatatgcaa
     1261 agttttactc aataacgatt actggtgtcg ggcattatca tttagtccta cgaaagcgaa
     1321 gaactatcag cgttataacg aacggataaa aggaaaacgt caggaatggg ggatactatg
     1381 caacaacgat taacgcagga tttaacgcaa tttctcgcca gcttgccgga agatgatcgc
     1441 atcaacgcga ttaatgaaat ccgcatggcg atccatcagg ttagcccctt tcgtgaagag
     1501 ccagtggatt gcgttctgtg ggttaaaaac agccaactta tgcctaatga ttacaaccca
     1561 aacaacgtgg caccacccga aaaaaaactg ctgaaaaaat ccattgaaat agatggtttt
     1621 acccaaccca ttgtggtcac gcatacggat aaaaatgccc tggaaatcgt ggatggtttt
     1681 caccgccatg aaattggtaa aggctcatca tcgttaaaat tacgcttaaa aggttatttg
     1741 ccggtgacct gtctgggagg tacgcgtaat caacgcatcg ccgcgactat tcgtcataat
     1801 cgcgctcgtg gacgccatca aatcaccgcc atgtcggaaa tcgtccgtga actcagccag
     1861 ttgggatggg acgataataa aattggcaaa gagctgggaa tggacagcga cgaagtgctg
     1921 cgcctgaagc aaattaacgg tctgcaagag ctgtttgccg accgtcaata ttcccgcgcc
     1981 tggactgtca aataactaca gctggcgcag tcgttcagct gctgccagca acgtcgattc
     2041 cttcttggca aaacagagac gaatcagttt atgtgggaag ggatcggcgc aaaacaccga
     2101 tagcggaatc gccgctacgc cgtgctcctg cgtcagccac tggcaaaact caacatcatc
     2161 cagggtagat acggcgctgt aatccaccag caaaaagtat gtgccttcgc acggtaaaat
     2221 ctccagccga ctttcattta aggcatttac cagaatatcg cgcttctggc gataaaagtc
     2281 cggtaacgca agataatgct caggttctgc acgtagcata tccgcaattg ccagctgtgc
     2341 cggggtattt accgaaaagg tcagatactg atgtaccttg cgaatttcgg cgctgatggg
     2401 cgctggcgca acgcaataac ccactttcca gccggtcata tgataggtct tgccaaacga
     2461 tgacaccgcc accgcccgct cacgcagctg cggatgcgcc agcacactgg catggccctg
     2521 ttgtgaaaag ttgatgtgct cgtagacttc atcgctaatg acaaaaatct cgtgcccggc
     2581 gatcgcctgc cacaaagcgg cgaaatcagc ctgctgccag acagttgcac tgggattatg
     2641 cggagtgttg aggatcacca gtctggtgcg ctcgcttaac aatgcggcaa attcctgcca
     2701 gtcaacgcga aaatgcggtg gttgcagtgc catacgcttc actattcccc cagaaagcgc
     2761 gatggcgggg gcgtaactgt catagctggg atcaaaacaa atcacttcat cgccattgcg
     2821 caccagtgcg gtaatcgccg cgtataacgc ttccgtcgcc cctgccgtta cggtgatatc
     2881 gctatcggca tctggttgat agccatacaa acgttccgtt ttctgagcaa tcgcctcgcg
     2941 caaggcctgc acgccggtca tgggcgcgta ttggtttgcc ccctgggcaa cgtggtacgc
     3001 cagccgctcc tgtaaatagc gcggaccatc aaaatcagga aagccttgcg acaggttaat
     3061 cgcctggtgt tgctgcgcca gcgcgctcat ctgggtgaaa atagtggtgc caagttgtgg
     3121 aagtttgctt tgtggaatca gagggttatt tgtcattttt attgtacctg acgaagttgc
     3181 gtgttgaagg cactataaca cgatgctagt atttggcaat caagacgttt agatgtctaa
     3241 ataaaacaat aaggacaaca caacatgcct cacaatccta tccgcgtggt cgtcggcccg
     3301 gctaactact attcacatcc aggaagtttc aatcacctgc acgatttttt cactgatgaa
     3361 aaactttctc gcgcggtgtg gatctacggc gaacgcgcca ttactgcggc gcaaaccaaa
     3421 cttccgccag cgtttgaact gccgggggca aagcatattt tgtttcgcgg tcattgcagc
     3481 gaaagcgatg tacagcaact ggcggctgag tccggtgacg atcgcagcgt ggtgattggc
     3541 gtcggcggcg gcgcactact cgacaccgcg aaagccctcg cccgccgtct cggtctgcca
     3601 tttgttgccg ttccgacgat cgccgctacc tgcgccgcct ggacaccgct ctccgtctgg
     3661 tataacgatg ccggacaggc gctgcattat gagattttcg acgacgccaa ttttatggtg
     3721 ctggtggaac cggagattat cctcaatgcg ccgcaacaat atctgctggc ggggatcggt
     3781 gatacgctgg cgaaatggta tgaagcggtg gtgctggctc cgcaaccaga aacgttgccg
     3841 ctaaccgtgc ggctgggaat caataatgcg caggccattc gtgacgtctt gttaaacagt
     3901 agcgaacagg cgctgagcga tcagcaaaat caacagttaa cgcaatcatt ttgcgatgtg
     3961 gtggatgcta ttattgctgg tggtgggatg gtcggtggtc tgggcgatcg ttttacgcgt
     4021 gtggcggcgg ctcatgccgt gcataacggt ctgaccgtgc tgccgcaaac cgagaagttt
     4081 cttcacggca ccaaagtcgc ttacggaatt ctggtgcaaa gcgccttgct cggtcaggat
     4141 gatgtgctgg cgcaattaac cggggcatat cagcgttttc atctgccgac tacgctggcg
     4201 gagctggaag tggatatcaa taatcaggcg gagatcgaca aagtgattgc ccacaccttg
     4261 cgcccggtgg agtccattca ttacctgcca gtcacgctga caccagatac gttgcgtgca
     4321 gcgttcgaaa aagtggaatc gtttaaagcc tgacgtgcaa atttagcagc agcgcgcgcc
     4381 gccttttcca ccgtagcgcg cgtcctgccg ctcacggaaa aactcttcgt aggtcatcgg
     4441 cgtttgatcg ggatggttaa cccgcatatg ttcgacatag ttgtcgtaat caggcatacc
     4501 aatcatcagc ttcgccgcct gacctaaata ttttccggct tttgccagtg aatcaaacat
     4561 caatacctcc ggtaatcagg ttgctgaaag agtgcattaa tagctatcaa cgcgctcggt
     4621 cagtcccctc gccccatcgg ggagagggtt agggtgaggg ggaaccctcg gcactatgcc
     4681 aacattcgcc aacatccccc ctcactctga ctttagtgcg cgcctttcgc ctgcgctacg
     4741 atctcctcaa cattttccgg catcggctca tacggcgttt ctttcgccgt tggcttcgga
     4801 tctttcaatg ccgcaagtgc cgtcttaatc gagaacagtg ccagaaccac cacgaccacc
     4861 ataaagaaga tggttaaccc ggcatccaga cggttgttga acaccagttg cgccagctgt
     4921 gactcagtat actgcgacgg aatattgccg ctgtcgatca ttgcctggaa cttgttagca
     4981 atggccagga agccgacttt cgcatccggg ctaaacgctt tctgccagcc agcggtcagg
     5041 gtacaaatca gcagccaggc cgttggtacc agcgccaccc aggcgtaacg ttgacgtttc
     5101 attttgaaca gcaccacggc acagagcatc agcgccatcc ctgccagcat ctggttggca
     5161 ataccaaaca gcggccacag agtgttaata ccacccagcg gatcgaccac accctgatgc
     5221 aggaagtagc cccacgccag tacgcacagc gccgttgcca gcaggttagc aggcagtgaa
     5281 tcggtccgtt tcaggcccgg agaaaccacg cccagcagat cctgcaacat aaagcgcgca
     5341 gcacgcgtac ctgcatccac cgccgtcaga ataaacaatg cttcaaacaa aatggcgaag
     5401 tgataccaga acgccacatc catcatgccg cccagcgcgc cgtgcagaat gtaggccatc
     5461 cccaccgcca gcgtcggcgc accgcccgca cgggaaatga tcgactgttc acccacttcg
     5521 ctggcaatct ggtttagcgt atccggggta atcgcaaagc cccagctact caccacttgc
     5581 gcggcagaag cgaccacatc cgccgtccct gccggagcca gcaccgccat cgggctgttc
     5641 atggcgaagt acacgcccgg atcgatgata caggcggaaa ccagtgccat aatcgccacg
     5701 aaggattcca ttaacattcc accgtagccg ataaagcacg cctgcccttc gttcgccagc
     5761 atcttcggcg tggtgccgga agagatcagc gcatggaagc cagacaccgc gccacaggcg
     5821 atggtgataa acaggaacgg gaacaggtta ccggtccata ccgggccagt gccatcgaca
     5881 aatttggtca gcgcaggcat ggtcagcgtc gggcgcataa tcaaaatgcc taccgccaga
     5941 ccgacgatag tcccgatttt caggaaggta gagaggtagt cacgcggggc cagcagcaac
     6001 cacaccggca gcaccgccgc cacaaaaccg taacccacca gcatccaggt cagctgcacg
     6061 ccggtaaagt caaagtacgg tgcccaggtc ggactttctg ccacccagcc gccagagata
     6121 atggcgaaaa tcaggaatac cagaccgatg accgacactt caccaatacg ccccggacgc
     6181 agatagcgca ggtagatccc cataaacagc gccagcggaa tggtgaacgc aacggtatac
     6241 gttccccacg ggctatgagt cagggctttc accacgatca tcgccagcac ggcaaggata
     6301 atgaccatga tcataaagca ggccaccagc gcaatcaccc cggcagttgg ccccatctct
     6361 tctttgacca gctcacccag cgatcgaccg tcacggcgcg tagaaacaaa cagcaccatg
     6421 aaatcctgca ccgcaccggc gagaaccacc ccggcgagca gccagatcat ccctggcagg
     6481 taacccattt gcgccgccag taccggcccc accagcgggc ctgctccggc aatggccgca
     6541 aaatggtgac cgaacagcac tttcttgtcc gtcggcacat agtccagccc gtcgttatgg
     6601 cgcaccgctg gcgtcatacg cgtcgggtca accgccagca catttttggc gatatacaga
     6661 ccatagaaac ggtaagcgat cagatagata cagaccgacg ccaccacaat ccacagcgcg
     6721 ttgatctgtt ccccacgatt taaagcaatg tatcccagag caaatgctcc cattacagag
     6781 agcactgtcc agacgaggta tttccctgat ttgttcatag ttgttatccg tgtgcgtgtc
     6841 catagagatg ttacattttg tttctataac atctttgtac gatttaacaa cacagcaacc
     6901 atgtaaatcg gagttgaaac caatatttaa cttaacaatg ttaactcgat cactccgtta
     6961 accgagtttt cctgcaatct catataattc tgtcgggttc atcccaaaac tgccgtaccc
     7021 agccgacaag tgcagcaacg ccgcccctgt tcatcgaaaa cgacgatttc ccagctctga
     7081 ttttgccgcc caagatgcag cggctggcag acgccgcgta ccttgccttc agacaccggg
     7141 cgatgatgtg tggcgttaag ttccgtgccc accacacact gcccgtcgcg ggtcatcata
     7201 aatccggcca tcgatcccag cgtttcagcc agcgccgccg acgcgccgcc atgcagcagg
     7261 ccaaacggct gatgagtacg ggtatcaacc ggcatttcgg cttccagcac atcatcgccc
     7321 agacgggtat acacaattcc cagatgcgcc accattgtgt tatcgctggt ggcgttcagt
     7381 tcgtcgagcg ttaaatggcg tttccagatc atgcttatgc ccccagcgtt gagccgccat
     7441 cgaccacaat atcctgtagg gtgatatggc tggcgaggtc agaggcgagg aacaaaatcg
     7501 tgttggcgat ctcttgtgga cgggcgattt tccccagcgg aatgccgagt ttaaactgct
     7561 cgccaaagcc gcgaatacgc tgttcttcgg cgtcatcgct cacccacagc gtgcgttgca
     7621 tatcggtatc ggtggaaccc ggcgaaacca cattacagcg cacgccgcta cccgccagtt
     7681 ccagcccgac gctcaacgcc aggcttttca gcgccgcttt cgatgcgcca taagcactca
     7741 taccaatacg cggcgtgtgt gcggcgtcgg acgccacagt gacaatcgcc ccgccccgct
     7801 gacggcgaaa ctggttcatg gtttgctgga acaggttaaa cgcaccgccg acgttaaccg
     7861 caaaagtctg ctgccagtcc tccttactga gctgatcggt cgcgcccatg cgcaaaattc
     7921 ccgccgcatt gaccagcacg tccagtcgct ccgtttcagc taacagtcgc tgacatacct
     7981 gcgccacctg cgcagcatcg gcaacatcca ttacttcggt cgcaaaggga tattgctcct
     8041 gagcgaacgc ttgatcaaaa cctgtaactt tcgctcccgc ttcaacaaac gccagcgccg
     8101 tggcgtagcc gatacctttc cccgcgccgg ttacccagac atttttaccg ctgaaatcca
     8161 ttatttcacc tcgcgggaga gtagcttcca ccaggcgtcg atggtcgggt ttttcgccaa
     8221 catgacaaag tcgatatcgc catgcacttt gcgccagcgc gccgccagcg ccatcatgcg
     8281 caccgaatcc agaccgtagt cgatcaggtt gtcgtcatcg aacggttcat cggactcgtc
     8341 cagcaacggc aggatcacct cacgcagcgc cgctttgctg gcggggatag gtgctggcag
     8401 taattcttcg gtcatcacca cccggccaga acgtccggcc acatatttca gcgacatcaa
     8461 atgctcgtca cggctgaaat cggccagcgc atccgccacc ataaacggtt taatatcgcg
     8521 cataaatgcg tcggttgcgg tggtcataca gccaatgtgg gcatataccc ctgtaataat
     8581 cagctggtta cgtccactct ctttgagcat ttgctccagc ggagaacgat gaaacgcgct
     8641 gtagcgccac ttcaccagca ccgtgtcgtc ggcatctggc gtcaggcgat ccaccacctt
     8701 ttgctgttcc ggcgagcggg tcaggcccgg cccccacata tcattcaaca gcgcccgatc
     8761 ttcatcgctc tgctctttcg gctgggcggt gtaataaacc gggatattgt gctgtttgca
     8821 gtagtcgcgc agcgcagcaa tattcgcgat cacctgcgcc atcatcgggc agttctcacc
     8881 ccagaagctg acaaaatagt cctgcatatc atggattaac aacgcggcac gttgcggttc
     8941 aaaggcccag tcgactttat tcttagggat atcgtgagac tccggcagtg cgtaagcctg
     9001 taattttgga atagccatcg tgttctctcc ttcaggctga cgcgcgtgac gccagccact
     9061 gacgtaattg ttttttatcg actttcccga ccgccgtcag cggaagtgaa tccacacact
     9121 ccacgcgatc cggtaattta aattcggcaa taccctgttc acgcaggaaa cgacgcacct
     9181 gcaccgcgcg cagcggctct tttaccacca gataagcaca gcttttttcg cccatcagct
     9241 catcttccat actgaccagt gcggcgtaga tcaccgccgg atggcgcagc aacaggtttt
     9301 cgatctcttc ggcagcgatc ttctcgccgc cacggttgat ctgatctttc tcgcgcccct
     9361 gcacggtgat gtaaccctct ggatcaatag agatcagatc gccggaacag taaaaaccgt
     9421 tggcatcaaa ggcgctggca ttgtgctgcg ggcttttgta atagccacgg aaggtgtacg
     9481 gcccgcgcgt catcaggcgt ccgacttccc cttgcggcag tggatttcct tcggcatcgg
     9541 caacccatac ttcatcgtcc ggacacattg ggtaaccctg ggtatggata attttctccg
     9601 cgctatcatc aagacgggta tagttcacca gcccttccgc catgccaaac acctgctgca
     9661 actggcagcc aatctcagag ggaatacggg ccgcaagcgt ggcagaaaga cgtgcgccgc
     9721 cgacctgtaa cagtttcagc gaggcaagct gcgcccggct ttcgccttcg gtcagcgctt
     9781 gcaaccacag gctgactgcc ggcggcacca gcgcggtaac gttaacctga tgttttgtaa
     9841 tcaatggaaa gcagagcgtg gcgctgggat cggccgccag aacaaccgtt cctccggcaa
     9901 gaaagacgcc cagcgatccc ggcgaactca tggcgtagtt atgagccgcc gggatcgcgc
     9961 acaggtagcg cgtctgttgt gtgaactgac aaatctcgac gctacgacgc acgctgtagt
    10021 agtagtcgtt atgagtgcgc gggatcagtt tcggtgtgcc ggtggtgccg ccggaaagct
    10081 ggaaataggc cacttcatca gcaggtgacg gcgtggcagt aaaatcctca gccggatggt
    10141 taatcgcatc ctgcaagtta tgctcaccgc tgtcgttgag cagatgcacc acgcgaatgg
    10201 aggaatgttc tgtgacgaaa gtattgagga aatcatcccc gctaaacagc gcatgttggc
    10261 gatcggcaat cagtaacgcc ggttcaatct ggctggcata ggcgttcagt tcactacgct
    10321 gatggctgaa cagcgccagc accggcgcaa cgcccagttt cagcagcgcg aaaaaggtaa
    10381 tgtaaagttc agcgacgtta cccagttgta ccagcgcggt ttcaccaggt ttaatgccct
    10441 gacggcgcaa actacacgcg aggttatccg ccgcctgatt cagctcccga taactcaact
    10501 gccgctcgcc gtcgataacc gcgatgctat cgctcgcggc gtggcgggtc agaatgtcag
    10561 tcagtggcaa atcctgccag tagccttttt cccgatagcg acgggcaaac tcttccggcc
    10621 agcgggtaaa tggaatgctc atcctcgctc cttaatgcaa tccaaaaacg ttcaacatgg
    10681 tagaaagttt gacgccggtt tcgcgccact cacccaacgg tgacgacgca ggtacaatcc
    10741 ccgctccggc aaacagacgc acctgatttt cccgcagctt cgcgcagcgg atggtcacca
    10801 cccattcgcc gttaccttcg ctgtcacacc aacccacaat gccgccaaac agctcgcggt
    10861 cgaacggttc cagttcagca ataacctggg tcgcggcctg atgcgggaaa ccgctcagcg
    10921 cgggggtcgg atgcagcaga caggccagag tcagtgcgtt ttcttgcgaa ttcgctttac
    10981 cttcaaaggg agttgcgaga tgccacagcg tcggcgtggt aatcaattgt ggagaggagg
    11041 gaacgtgtaa ctcactactg cgttcgcgca gtacctcttt catcgcctga gtcaccaatt
    11101 catgttcatg gcgatctttt tctgacgcca gcagacgatt acccgcttcg cgatcgagca
    11161 cttcatccgg ctgacgacgc gcggaaccgg ctaacggaat ggagctaaaa cgctcgccgt
    11221 ctttacgtag cagcagttcc gggctggccc ccagcaggac gccaccatca gccagcggga
    11281 catggaagtt gtaactaacc gggttttgcg caatcaaccg ttccagcaat acgccactat
    11341 caatggcggc gtcagtggtg atatcaatca accgtgacaa caccactttg tcgacctgcg
    11401 gcgtggcggt aagtgcggcg gcgcgggcaa ccatctgttc aaacgtggtt tgctccggaa
    11461 ttgcctggcg ttccaccaca ttcagcaact ggctgcgggt gaaacggcgt gcggaagttt
    11521 gtttttcctg acgggagaac gactgccagg attcgggaat atacagcgac gaaggctgac
    11581 gtggatcgaa gggaatcgcc ccaaccatca ccggattttt gatgccctgc gctttggcat
    11641 cggcaaacag cgcggcgagt ttttgctgga agggactgtc gggcgaatcc ccgttcacag
    11701 ccggttcatc gaagcgggcg aaacatcctg acgtcgtaaa actgcggtac ggcgacataa
    11761 agaaaaagcg attgggcgca agtgttgcca tggtctgctg tacttcctca gccagtgacg
    11821 tatccatatc atcctccaca aaatgataaa ggctttaata atgattatca tttatatttt
    11881 cggtcgctaa cctaaaagca aacgcgcact atgtcaactc ttgaggtaac gcaatctgca
    11941 actcggggtt gcatctggtt gtggttacaa tgaaaatgag aagcattaac ttcattaata
    12001 acaggaagct gatttgtgag actcgccccg ctctaccgca acgcccttct attaacagga
    12061 cttttgcttt caggaatatc cgcagttcag gccgccgact ggccgcgtca gattactgac
    12121 agccgtggca ctcataccct ggaaagccag ccgcagcgta ttgtttccac cagcgtcacc
    12181 ctgaccggct cactgctggc gattgatgct ccggtgatcg ccagcggcgc gaccacgcca
    12241 aataaccgcg tagcggatga ccagggcttt ttacgccagt ggagcaaggt ggcgaaagaa
    12301 cgcaaactac aacggctcta tatcggcgaa ccgagcgccg aagccgttgc cgcgcaaatg
    12361 ccggatctga ttttaattag cgcaactggc ggggattcgg cgctggcgct gtatgatcag
    12421 ctttccacca tcgccccgac attaatcatc aattacgacg acaaaagctg gcaggcgctg
    12481 ttaacgcaac ttggcgaaat taccgggcat gagaaacaag cggcagagcg gattgcgcag
    12541 tttgataagc aactggcggc ggcgaaagag caaatcaaat taccgccgca gccggtcact
    12601 gccattgtct atactgccgc tgcacacagt gccaatctct ggacgccgga atcagcacaa
    12661 gggcagatgc tggaacaact cggctttacg ctggcgaagt tacccgcagg gttaaatgcc
    12721 agccaaagcc agggtaaacg ccatgacatc attcagcttg gtggggaaaa tctggctgct
    12781 gggttaaacg gtgaatcgct atttctgttc gctggtgatc agaaagatgc cgatgcgatt
    12841 tatgccaatc cactgctcgc gcacctgccc gcagtacaaa acaagcaggt ttatgcgctg
    12901 ggaaccgaga cgttccgcct ggattactac agcgccatgc aagtgctgga acgacttaag
    12961 gcgctgtttt aagcattaac tgtcagacgc tgtcacctgc ggcggcgtct ggcgaaaacg
    13021 tcgcaactcc accagcacca gcaataacaa cacgccgata atcaacaaac caaaaccgct
    13081 cgcgctcgcg gaagcaaccg gtgtcatcat cgcgcccaaa ccgcccagca gcgccgcgcc
    13141 tatggcatcg cccgtcacgt tttgcgccgt ccacaaaccg ttaatccgcc ctaacatcgc
    13201 ttccggggtt tgcgtttgca gcattgtata ttgcagcaac gaactaaccg cactcagcca
    13261 gccgaacagc gccagacaaa ccacgcctaa aatccacatc ggcatcaggc caaacagacc
    13321 aatggcgagg aacgatccca gcgtggagag cagcatcaat aaccctggtc gcgcgctatg
    13381 cgccagcttc ccgctggtta acgcgccaat agccgcgccg agtgggatcg ccgcgtagag
    13441 aaaaccaatc tgtgccgctg acatctgcca gttgtcagcc agcgccggat acagtacccg
    13501 caccgcgctc gccatcgtca ataaaccacc cagcagcgca atccctccta ccagcgggct
    13561 ggcgagcaga aaacgaaatc ctgccagtaa tgatttcaac ggatgctcac gcggctgcgg
    13621 tggcggtggc agtgccggaa ggcttaacaa cggtagcaag gtaataaacg tgcccgccgc
    13681 cgccagcccg tagttccagg ccacaccgcc agtcgccagc agtaaaccgc caatcatggg
    13741 cgaaatcacc gaccccagac gcacggtcaa catggtgatc gccccggcct gcattaagtt
    13801 ttcacgccct accagtgctg gtgtcgccgc caatagcgcc gtaacgccaa gcgatgcgaa
    13861 aaaaccatcc cataaaccaa gtaaatagat tgccagcaat gacggctccg gcagcagcgc
    13921 gttcaggcac agtccaatga agccaatgcc acaggtgccg cgcgccagca aaatcacttt
    13981 tttgcgctca tagcgatccg ccagcacacc gccgaccatc aggccaacaa acatcgcgcc
    14041 gccggtcagc gtcaccgaaa gccccacctg ccaggtggaa tgtgtcatca tctggatctg
    14101 caccggcacc gcgacgccga gcaaacccag agaaacaata gagatgaaac gagcgaggaa
    14161 tactgcgcga aacgccgggt gcgttttcaa caggctgagg ttaagcagcc aggattgttt
    14221 attcattaca atgccttgcc atcaaatatt ttaaatacct attcttaggc tgcacatgct
    14281 aacatatcca aataagatcg ataacgataa ttaatttcat tatcatggaa gttcgtatgt
    14341 ctggttctgt tgccgtgaca cgcgccattg ccgtgcccgg attgctgtta ttactgatta
    14401 tcgcgacggc attaagtctg ctcattgggg caaaatcact ccccgcttcc gtagtgctgg
    14461 aggccttctc cggcacctgc cagagcgccg actgcaccat cgtgctcgac gcgcggctgc
    14521 cgcgtaccct tgccggttta ctggcaggcg gcgcgcttgg ccttgccggg gcgttaatgc
    14581 aaaccctcac ccgaaaccca cttgccgacc ccggcttgct tggcgtgaac gccggagcca
    14641 gctttgccat tgtgctgggt gcggcgctgt ttggttactc ttccgcacag gaacaactgg
    14701 cgatggcctt cgccggggcg ctggtggcct cgttgattgt tgcctttacc ggcagccagg
    14761 gcggcgggca gttaagtccg gtgcgtttaa ccctggcggg cgtagcgctg gcggcggtgc
    14821 tggaagggct gaccagcggc atagccctgc ttaatcctga cgtctacgat caactgcgtt
    14881 tctggcaagc cggttcgctg gatattcgca atctacatac cttaaaggtg gtgctgatcc
    14941 cggtgctgat cgccggagca actgcgctat tactgagtcg cgcgctgaac agtttgagcc
    15001 tcggcagtga caccgcgacg gcgctgggca gtcgcgtggc gcgcacacag ttgattggtc
    15061 tgctggcgat taccgtgctt tgtggtagtg cgacggcggt agttggcccg attgccttta
    15121 ttggcctgat gatgccgcat atggcgcgtt ggctggtggg tgccgatcat cgctggtcgc
    15181 tgcccgtcac gctacttgct acccctgccc tgctgctgtt tgccgatatc atcgggcggg
    15241 tgattgtgcc cggcgaactg cgcgtttctg tggtcagtgc gtttattggt gcaccggtgc
    15301 tgatcttcct cgtgcgacgt aaaacgcgag gtggtgcatg atttacgtct ctcgccgatt
    15361 actcatcacc tgtttgctgc tggtttccgc ctgtgtggtt gcaggtatct ggggattacg
    15421 cagcggtgcc gtcacgctgg aaacctcgca ggtattcgcc gcgctgatgg gcgatgcgcc
    15481 gcgaagtatg acgatggtgg tcaccgaatg gcgtttacca cgcgtgctga tggcgctgtt
    15541 gattggcgcg gcactgggcg tcagcggcgc gatttttcag tcgctgatgc gtaacccgct
    15601 cggcagccct gacgtaatgg gctttaacac cggggcgtgg agcggcgtgc tggtggcgat
    15661 ggtgctgttt ggtcaggatc tgacggctat cgcgctggca gcaatggcgg gcggcattgt
    15721 cacttcgctg ttggtctggc tgctcgcctg gcgtaacggt atcgacacct ttcggttgat
    15781 tattatcggt atcggcgttc gcgccatgct ggtggccttt aatacctggc tgttgctgaa
    15841 agcgtcttta gaaacggcgc taacagcggg tttgtggaat gccggatcgc tcaatggcct
    15901 gacgtgggca aaaacctcgc cctccgcacc catcattata ttgatgctca ttgccgccgc
    15961 cttgctggtg cggcgaatgc gcttgctgga aatgggcgat gacaccgcgt gtgcactggg
    16021 cgtcagcgtc gaacgttcgc gtctgttaat gatgctggtt gcagtggtgc ttaccgctgc
    16081 ggcaacagcg cttgccgggc cgatttcctt tattgcttta gtcgcgccgc acattgcccg
    16141 acgcattagc ggcaccgctc gctgggggct aacccaggcg gcgctgtgcg gggcgttgtt
    16201 actgctggcg gccgatctct gcgcccaaca actgtttatg ccgtatcaac ttccggttgg
    16261 cgtcgttacc gtcagcctcg gcggtattta ccttatcgtc ttgttaattc aggagtctcg
    16321 caaaaaatga ccgaatcagt agcccgtttg cgcggcgaac agttaaccct gggatatggc
    16381 aaatataccg ttgcggaaaa tctgactgta gaaatacctg atggtcactt cacggcaatt
    16441 atcgggccaa atggctgcgg taaatccacg ttactgcgga ccttaagccg cctgatgacg
    16501 cctgctcacg ggcatgtctg gctggatggc gagcacattc aacattacgc cagtaaagag
    16561 gtcgctcgcc ggattggtct gctggcgcaa aacgctacca cgccgggaga tatcaccgtg
    16621 caggagctgg tggcgcgtgg gcgttatccg catcaaccgc tgtttacccg ctggcgcaaa
    16681 gaggatgaag aagcggtaac gaaagcgatg caggccacgg gaataactca tctggcagat
    16741 caaagcgtgg ataccctttc tggcggacaa cgccagcgag cgtggatcgc gatggtgctg
    16801 gcccaggaaa cggcaattat gctgctcgac gaacccacga cctggctgga tatcagtcat
    16861 cagattgatt tgctggaact gttaagcgaa ctgaaccgcg agaaaggcta taccctggcg
    16921 gcggtgctgc acgatcttaa tcaggcctgt cgttacgcca gccatttgat tgcattgcga
    16981 gaagggaaaa ttgttgctca gggagcgccg aaggagattg tcactgctga actgattgag
    17041 cgcatttatg gtctgcgttg catgatcatt gacgatccag ttgccggaac gccactcgtg
    17101 gtgccgctcg gacgaacggc accttcaacc gcaaatagtt aaactaagtg gtctgccatc
    17161 atggcatcct gttttctgga tgccatcgca tagcgcaata acacgctacc acaagccacc
    17221 atcccgccga tcagcgcgga aaggatcaca ataatcgcct tacccggacc gtcttttttc
    17281 actggcaatg acgggcttaa ctgatattta aacggcgtaa aattcacatc gttgatattt
    17341 gcttttgtta actgctcaac aagatactga cgattacgta attcaccgtt cagttccgca
    17401 acgtcagtga ccgctttttc aatttccagt ttgcgttcaa taccgtctgc gccgagagaa
    17461 atagagaaat cggggtcatc tttaacggcc tgaccattac tgtaaacggg ctttttaatt
    17521 cctgccgcgt tggcaatgtc gagtgaataa ttgaggcgct gaatgtttgc atcaagttga
    17581 tttttcattt taatgcggtc ctgagccagt ttttcttttt caaactgggt tttgatctcc
    17641 agtttattac ggacgttttc tatcgactct ttcaccacca acgcagagat ataatcgata
    17701 tacccgctca aaacggtctg cgcctcttca ctggttggcg cggtaaaact tagcgtccag
    17761 gaggtataca gtgacggttc atcttttttc ttactggcat tgtcatcaac cgctttcatt
    17821 ttttcgctca atgcgacaat tgcgcgatgc aaatccagtt cgtcgatttt cgcctctttt
    17881 aattggtcca tcacataagg tgatgaacgc aggtactctt ccagcaagct aaccgactga
    17941 aacttcttga taaacaggtt aaatgcttct gtacgatcaa ttttgatatc cagatccagc
    18001 acacgaagct tagtgaatgt tttctccaac tcttgccact gaacaggttc tggaggcgtg
    18061 acaaccgccg cgctggtcca tttttgcggc aggatgaaag agatcagcaa gcctgcgcag
    18121 gcaaacgcaa aaacgaccgc catgaccgtt tttttggccc gccataaaac tgagattaga
    18181 ttaagtaaat
//
LOCUS       DAZUCE010000092        18096 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZUCE010000092 DAZUCE010000000
VERSION     DAZUCE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.092"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(192..1784)
                     /gene="lsrK"
                     /locus_tag="ACHY03_002647"
     CDS             complement(192..1784)
                     /gene="lsrK"
                     /locus_tag="ACHY03_002647"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFS3905856.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTLDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1863..2816)
                     /gene="lsrR"
                     /locus_tag="ACHY03_002648"
     CDS             complement(1863..2816)
                     /gene="lsrR"
                     /locus_tag="ACHY03_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFS3905857.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3065..4600
                     /gene="lsrA"
                     /locus_tag="ACHY03_002649"
     CDS             3065..4600
                     /gene="lsrA"
                     /locus_tag="ACHY03_002649"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFS3905858.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGSNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRFSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KYNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4594..5622
                     /gene="lsrC"
                     /locus_tag="ACHY03_002650"
     CDS             4594..5622
                     /gene="lsrC"
                     /locus_tag="ACHY03_002650"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFS3905859.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5622..6614
                     /gene="lsrD"
                     /locus_tag="ACHY03_002651"
     CDS             5622..6614
                     /gene="lsrD"
                     /locus_tag="ACHY03_002651"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFS3905860.1"
                     /translation="MRIRYGWELALAALLVIEIVSFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6626..7648
                     /gene="lsrB"
                     /locus_tag="ACHY03_002652"
     CDS             6626..7648
                     /gene="lsrB"
                     /locus_tag="ACHY03_002652"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFS3905861.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7675..8550
                     /gene="lsrF"
                     /locus_tag="ACHY03_002653"
     CDS             7675..8550
                     /gene="lsrF"
                     /locus_tag="ACHY03_002653"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFS3905862.1"
                     /translation="MADLDDIKDGKDFRTDQPQKNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8574..8864
                     /gene="lsrG"
                     /locus_tag="ACHY03_002654"
     CDS             8574..8864
                     /gene="lsrG"
                     /locus_tag="ACHY03_002654"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFS3905863.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8921..9679
                     /gene="tam"
                     /locus_tag="ACHY03_002655"
     CDS             8921..9679
                     /gene="tam"
                     /locus_tag="ACHY03_002655"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFS3905864.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPGARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLSDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9683..10597)
                     /locus_tag="ACHY03_002656"
     CDS             complement(9683..10597)
                     /locus_tag="ACHY03_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFS3905865.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGIENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10794..12245)
                     /gene="uxaB"
                     /locus_tag="ACHY03_002657"
     CDS             complement(10794..12245)
                     /gene="uxaB"
                     /locus_tag="ACHY03_002657"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFS3905866.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKMTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDSFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12472..13890)
                     /locus_tag="ACHY03_002658"
     CDS             complement(12472..13890)
                     /locus_tag="ACHY03_002658"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS3905867.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14029..14388)
                     /locus_tag="ACHY03_002659"
     CDS             complement(14029..14388)
                     /locus_tag="ACHY03_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFS3905868.1"
                     /translation="MQSLDTLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14388..15314)
                     /gene="glsB"
                     /locus_tag="ACHY03_002660"
     CDS             complement(14388..15314)
                     /gene="glsB"
                     /locus_tag="ACHY03_002660"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFS3905869.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15378..16766)
                     /gene="sad"
                     /locus_tag="ACHY03_002661"
     CDS             complement(15378..16766)
                     /gene="sad"
                     /locus_tag="ACHY03_002661"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFS3905870.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNAHNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16867..17748
                     /locus_tag="ACHY03_002662"
     CDS             16867..17748
                     /locus_tag="ACHY03_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3905871.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            17826..>18096
                     /gene="yneK"
                     /locus_tag="ACHY03_002663"
     CDS             17826..>18096
                     /gene="yneK"
                     /locus_tag="ACHY03_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFS3905872.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICNE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLT"
BASE COUNT         4548 a         4604 c         4503 g         4441 t
ORIGIN      
        1 ttcgctataa aataagggtt atgcttaatg ctttaagaaa aatagcaatt ttcccttgaa
       61 tatagtactg gtgatgaaac gatgaatctg caagctggca tttaacaagc cagctctaaa
      121 agaagggaaa taagaataac tatactcaaa aactaacagc cacggtcatc atgatgtggc
      181 tgtcaatgaa actataaccc aggcgctttc cataacgacg tcgtcagtcc atgatcaacc
      241 agccccagct gatcctgata aactgcctgc cacttatcgc gtgaatcctg ataaagttca
      301 tgcttttccg ggtctagtgt gtgcgtccgt tcccagcgaa ccaggcgttc tccggtttct
      361 gccattgatg aaaaaattcc ggcaccgacg ccagctgcaa tggcacatcc taatgcagtg
      421 gcttctttga ccaccggaat attgacgggt aatcccgaga catcagcgag aatttgactc
      481 cataatttcc cttttgaacc tccgcctgca aagactaacg atgaaggatg aatattcgaa
      541 aaatcagcaa tttgctgcaa gttacacgct gatacaatcg ccgcattttc ttccagcgca
      601 cggaacaatg tcgctttgtt acatttatcc gggtcaatgg acaaattaat aaaggaaggc
      661 gcagcgtgat accaggtttt aaagcgcatt ctgtcggaga agatcggcat tacgccccac
      721 gacccaggcg gcacccgact ggccatctct tccagcagcg tataggtgtc gatgcctaaa
      781 cgttcggcaa tcagtttttc ttcggcacag aaagcatcgc ggaaccagcg catggtgagt
      841 ccggtaaaaa agcttataga ttcagcttgt accatgccag gaataacatg aggattaacg
      901 cgcacgttca tttctgggtc tgtcaccggc gcggctaaat ttacaacttg ctgccagaat
      961 gtgccgccaa gaaccgcggt ttgtgccgga cgcacaacgc ctaaccccag gcaaccaagc
     1021 tgcacgtcgc ctcctccaac gaccaccgga gtgcccacct tcagaccgca gagttccgcc
     1081 gcttgtgaac ttaccacgcc cagcaatgtg ccggtttctt tgacaggaga aagaatatcg
     1141 gcacgcaggc cagccatatc cagcaatgca ggtttccagt cacggatggt tagatcaaga
     1201 agtcccgtgg tgccagcgtt agagggatcc accgcaagtt cgccgctgag catataggcc
     1261 agccagtcgc tgatcatggt gatggttgat gcctgacggt aaatatcgga acgatggtgc
     1321 gccagccaaa gtaatctggg gatggcactt aaagccagtg tttgtccggt cgcgcgataa
     1381 acttcgtttt caaaggtatt gttgtgcagt tctttaagtt cgctaacttc gcgcgccgct
     1441 ctggcatcca cattggcgca ggcccagatc ggtgttcctt cattgttata taaaacaatg
     1501 ccttcacgca tcgaacatgc cgaaacggca gcgatatact ccggggctat gccggcgttg
     1561 tgcaacgcct ggcgcataca ctcacacgcc agttgccagt ttttgttgag atcaaattcc
     1621 atagaaccag gaacgtccgg tactgccaga tgccgccact ccgcctgtcc cactgctatt
     1681 tgattgcctt ccaggtcgaa tatcacagcc cgaatacttc cggtgcctgc atccagcgcc
     1741 atcaggtagt actttgattc tgaaggggta aagagtcgag ccattagatt atcctcggct
     1801 tatgcgttct gtctgttcct ctatacgttc tccatcattc ccggtaataa ggtcatgcaa
     1861 atttaactac gtaaaatcgc cgctgctgtg tcctgatcgg taaccagtgc gttgatataa
     1921 ccgcctttca ttgcagcggc aattgcttcg gctttatttt ctccccctgc cacgccaacc
     1981 cggacgggta tggtcttcag cgcgcttaaa ggtaagccaa tcagttcgtt atgtattttg
     2041 atatccgtga caacgtcacc ttttgcatca aaaaagtagc ctaaaatgtc gccaaccgcc
     2101 ccttttcggc caatcattaa ctgttcgccc tggctgatat aaccggagcg aatgatcgtc
     2161 gcgtcgtctt gttgactcac ggccccaatg ccgacaatcg ccacatccgc tgcttgcgcg
     2221 gctaacagaa catctttgac gcaattttca ttttttagcg tacgggcaat gtcagcggag
     2281 gatgcccgca acggagccgg aataatattc acactgcacg ccgcattaag ctgtccgata
     2341 cccgtcatat aagaaccgac gccaccggag agcgtgacca ggcgaatttg ctgtgacgaa
     2401 ataaaaccac ttaagcgttg cagcgtattc atggttgcct cgccaaaacc aatcgccagc
     2461 atctgttgtg gttgaagtaa actcatcaac atatgcgccg cacctatccc tagtcgcccg
     2521 ccgacatcag catccgcaag cccagggatc acccggacat gttgcagcga aaactgacga
     2581 cgtaattgag tttcatattc cagacagcct tcaaagcgag aattaatctg tacgcgaata
     2641 atgccggact gatgcccttt ctccagtaat cgcgacactt tcaaacgtgt caggccgaga
     2701 cgatcgctga tctcgctctg ggtcagcccg tcgtgatagt aaaaccacgc gatccgcgcg
     2761 acctgttctt cttcacacat tccctgttct gaaattaccg aatcgttgat tgtcataatt
     2821 cattcttcac tttgaacata tttaaatctt taatgcaatt gttcagttct tgcgcattta
     2881 tatctgtgat ggcaaccaca gtttgactct acgagcatga acaaacgcaa ccgtgaaaat
     2941 caaaatagca taaattgtga tctattcgtc ggaaatatgt gcaatgtcca cctaaggtta
     3001 tgaacaaatt aacagcagaa atacatttgt tcaaaactca ccagcaaaac tgaacggggg
     3061 aaatatgcaa acgagtgata cccgcgcgtt accgctactt tgcgcccgct cggtttataa
     3121 acagtattca ggggtcaatg tcctgaaagg catcgatttt acgttgcatc agggggaggt
     3181 ccacgccctg ctcggcggca atggtgccgg taaatcgacg ttaatgaaga ttattgccgg
     3241 tattacccct gctgatagcg gtacgctgga gattgggggc agcaactaca ccagattaac
     3301 gccagttcat gctcatcagc tgggtattta tctcgttccc caggaaccgc tgcttttccc
     3361 aagcctgtcg ataaaagaaa acatcctgtt tgggctggca aaaaaacagc tctccatgca
     3421 gaaaatgaag aacttgctgg cggcgctggg ctgccagttt gatctgcata gtctggcagg
     3481 atcgctggat gtcgccgatc gccaaatggt ggaaatcctc cgcgggctga tgcgcgactc
     3541 gcggattctg atcctcgatg aacctaccgc ctcgcttacc cctgcggaaa ccgaacgctt
     3601 gtttagtcgc ttgcaagagc tgcttgctac tggcgtgggt attgttttta tctcgcataa
     3661 gctgccggaa attcgccaga ttgccgatcg aattagcgtg atgcgcgacg gaaccatcgc
     3721 cttaagcggc aaaaccagcg aactgtctac cgacgacatt attcaggcca tcaccccagc
     3781 ggtacgggaa aaatcgctct ctgccagcca aaaattatgg ctggagttac ctggcaaccg
     3841 cccacaacat gccgcaggaa cgtcggtgct gacactggaa aatctgactg gcgaaggttt
     3901 caggaatgtc agcatgacgc taaatgccgg agaaattctg ggtctggctg ggctggtggg
     3961 tgccggacgc acagaactgg ccgagacgct ctatggtcta cgtactttgc gtggcggacg
     4021 cattatgctg aatgataaag agatcaatag attctccact ggagaacgtt tactgcgcgg
     4081 tctggtttat ctgccggaag atcgccagtc atccggactg aatctcgatg cttcgctggc
     4141 ctggaacgtc tgcgccctta ctcataacct tcgtggattc tgggcgaaaa ccgcgaaaga
     4201 taatgccacc ctggaacgtt atcgtcgggc gctgaatatt aaatataacc aaccggaaca
     4261 agctgcacgg acattatccg gtggtaacca gcaaaaaatc ctcattgcca aatgtctgga
     4321 agcttcgccg caagtattga ttgtcgatga gccgacgcgc ggcgtggatg tctcggcccg
     4381 taatgatatc taccagctgt tacgcagcat cgccgcacaa aatgtggctg tgctgcttat
     4441 ctcctccgac ctggaagaga tcgaactgat ggcagatcgt gtgtatgtga tgcatcaggg
     4501 cgaaattacc cactctgcac tgaccgggcg cgatattaat gtcgagacta ttatgcgcgt
     4561 tgccttcggc gatagtcagc gtcaggaggc gtcatgctga agtttattca gaacaaccgt
     4621 gaaatcacgg cactgctggc ggtggtgctg ctgtttgtat tacccggttt tctcgaccgc
     4681 cagtatttaa gtgtgcaaac gctgaccatg gtttatagca gcgcgcaaat cctgatcctg
     4741 ctggcaatgg gcgcgacgct ggtgatgctt acgcgcaata ttgatgtttc agtgggttcg
     4801 attaccggaa tgtgcgcggt gctgttgggg atgttactga acgcggggta ttcactacct
     4861 gttgcttgtg tcgcgacttt actgcttggt ttgctcgcgg gatttttcaa cggtgtcctg
     4921 gtcgcgtggc taaaaatccc tgccattgtt gccacccttg gcacgttagg gttgtacaga
     4981 ggcatcatgt tgctgtggac tggcggtaaa tggattgagg ggttacccgc cgaactgaaa
     5041 cagctctccg ccccgctact gtttggcgtt tccgcgatag gttggttgac gataattctg
     5101 gtggcattta tggcctggct gctggcaaag acggcgtttg gacgcagttt ttatgccacg
     5161 ggcgataatt tacagggcgc tcgtcaactg ggcgttcgta cggaagccat tcgcattgtg
     5221 gcattttcgt tgaacggctg catggcggca ctggcgggga ttgtgtttgc ttcgcagatt
     5281 ggttttatcc ccaaccagac cggtaccggg ctggagatga aagcaattgc agcctgcgtg
     5341 ctgggcggca ttagtttgct cggcggttcc ggtgcgatca tcggtgcggt actcggcgca
     5401 tggttcctga cgcagatcga tagcgtactg gtgctgttgc gcattccggc atggtggaat
     5461 gattttatcg cgggtctggt tctgctggcg gtgctggtgt ttgatggacg cctgcgttgt
     5521 gcgctggaac gtaatctgcg gcggcaaaaa tatgcccgct ttatgacgcc accgccatcc
     5581 gttaaacccg cttcgtcagg taaaaaacgg gaggctgcat aatgcgtatt cgctacggtt
     5641 gggaactggc tcttgccgca ctgcttgtta ttgagattgt ctcatttggt gcaattaacc
     5701 cgcggatgtt agatctcaat atgttgctgt tcagcaccag tgactttatc tgcattggca
     5761 ttgtcgccct accgctgacg atggtgattg tcagtggcgg gatcgatatt tcgtttggtt
     5821 cgaccatcgg cctctgcgcc attgcattgg gcgtactgtt tcaaagtggt gtgccgatgc
     5881 cgctggcgat tctcctgacc ttactgctcg gcgcattgtg cgggctgatc aacgccggat
     5941 taattatcta taccaaagtt aacccgctgg tgattacgct tggcacgctg tatctgtttg
     6001 ccggaagcgc cctgctgctt tccgggatgg ccggggcgac ggggtacgaa ggtattggtg
     6061 gattcccgat ggcgtttaca gatttcgcta acctggatgt gttggggctc cccgttccgc
     6121 tgattatctt cctgatatgt ctcctcgttt tctggctctg gctgcataaa acccatgccg
     6181 gacgtaacgt gtttttgatt gggcaaagcc cgcgcgtggc gctttatagc gcgattccag
     6241 ttaaccgtac cttatgtgcg ctctatgcca tgacggggct ggcgtctgcg gtcgccgctg
     6301 tgctgctggt gtcgtatttt ggttcagcac gttccgatct cggtgcgtcg tttctgatgc
     6361 ccgccatcac cgccgtggtg cttggcggtg ccaatattta tggtggttcc ggttccatta
     6421 tcggcaccgc cattgcggtt ttattagtgg gatatttgca acaaggtttg caaatggcag
     6481 gagtgccaaa tcaggtgtcc agcgcccttt ccggtgcgct acttatcgtc gttgtcgtag
     6541 gtcgttccgt tagcctgcat cgccagcaaa ttaaagagtg gctggcgcgt cgggccaata
     6601 acccattgcc ataaaggata tcttcatgac acttcatcgc tttaagaaaa tcgccttact
     6661 tagcgctctt ggcattgccg caatctctat gaatgtgcag gccgcagagc gtattgcatt
     6721 tattcccaaa ctggttggcg tgggattttt taccagcggt ggcaacggcg cacaacaagc
     6781 gggtaaagag ctgggcgttg atgtgaccta cgacgggccg acagaaccca gtgtttctgg
     6841 tcaggtacag ttgattaata acttcgtcaa tcaaggttat aacgccatta tcgtttctgc
     6901 ggtttcgcct gatggcttgt gtccggcact gaaacgcgcc atgcaacgtg gtgtgagagt
     6961 gctgacctgg gactctgata ctaaaccgga gtgccgctct tactacatta atcagggaac
     7021 tcccgcccag ttgggaggta tgttggtgga tatggcggcg cgtcaggtga ataaagacaa
     7081 agccaaagtc gcgtttttct actcaagccc caccgttacg gaccaaaacc agtgggtgaa
     7141 agaagcgaaa gcgaaaatcg ccaaagagca tcctggctgg gaaattgtca ctacgcagtt
     7201 tggctataac gatgccacta aatcattaca aaccgcagaa ggaatattaa aagcgtatag
     7261 cgatctcgac gccattatcg cccccgatgc caatgccctg cccgctgccg cacaagccgc
     7321 agaaaacctg aaaaatgaca aagttgcaat tgtcggattc agtacgccaa acgtgatgcg
     7381 tccatatgtg gaacgcggca cggtgaaaga atttggcctg tgggatgtgg ttcagcaagg
     7441 caaaatttca gtttatgtcg cggatgcatt attgaaaaaa ggatcaatga aaacgggcga
     7501 caagctggat attcagggcg taggtcaggt ggaagtctcg ccaaacagcg tccagggcta
     7561 tgactacgaa gcggatggta atggcatcgt actgttaccg gagcgcgtga tattcaacaa
     7621 agagaatatc ggcaaatacg atttctgatg tgcattactt aaccggagta agttatggca
     7681 gatttagacg atattaaaga tggtaaagat tttcgtaccg atcaaccgca aaaaaatatc
     7741 ccttttaccc tgaaaggttg cggtgcgctg gattggggaa tgcagtcacg cttatcgcgg
     7801 atatttaatc cgaaaacggg taaaaccgtg atgctggctt ttgaccatgg ttattttcag
     7861 ggaccaacta ccggacttga acgcattgat ataaatatcg ccccgctgtt tgaacatgcc
     7921 gatgtattaa tgtgtacgcg cgggattttg cgcagcgtag ttcccccggc gaccaataag
     7981 ccggtggtac tgcgggcgtc aggtgcgaac tctatcctgg cggaattaag caatgaagcc
     8041 gtggcgttat cgatggatga cgccgtgcgc ctgaacagtt gcgcggtggc ggcgcaggtt
     8101 tatatcggca gcgaatatga acatcagtcg atcaaaaata ttattcagct ggtcgatgcc
     8161 ggaatgaaag tgggaatgcc gaccatggcc gtgactggcg tgggcaaaga tatggtgcgc
     8221 gatcagcgtt atttctcgct cgcgactcga atcgccgctg aaatgggggc gcaaattatc
     8281 aaaacctatt atgtcgaaaa aggttttgaa cggattgttg ccggatgtcc ggtacccatt
     8341 gttattgctg gcggtaaaaa attaccggag cgcgaggcgc tggaaatgtg ctggcaggct
     8401 atcgatcagg gcgcttctgg tgtggatatg gggcgtaata ttttccagtc tgaccatccg
     8461 gtggcgatga tgaaagccgt acaggcggtg gttcaccata acgaaacggc tgatcgggca
     8521 tatgaactct atctgagtga aaaacagtaa ctgcggattt aaggagaaga attatgcacg
     8581 tcacactggt tgaaattaac gttcatgaag acaaggttga cgagtttatc gaagtttttc
     8641 gccagaacca cctgggctcg gtacaggaag aaggcaattt gcgcttcgat gtcttacagg
     8701 acccggaagt gaattcgcgc ttttatatct acgaagccta taaagatgaa gacgcagtgg
     8761 cgttccataa aaccacgccc cactacaaaa cctgtgtcgc gaaactggaa tctttaatga
     8821 ccgggccgcg taaaaaacgt ctgttcaatg ggttgatgcc gtgaggcgaa tttatcaatt
     8881 ttatctacaa ttggggtaac gcgctgacgg gagtaaaaaa atgtctgact ggaacccctc
     8941 tttatatctg cactttgccg ctgaacgatc gcgtccggcg gtggagctgc ttgccagagt
     9001 gccgctggag aatgtcgaat atgttgccga tcttggctgt ggcccaggta acagcaccgc
     9061 cctgctgcat caacgttggc ctggtgccag gataacgggc atcgactcgt ctccggcgat
     9121 gattgctgaa gcgcgcagtg ctttgccaga ctgccagttc gtggaagcag acatccgcaa
     9181 ctggcaaccg gaacaggcac tcgatctgat ttttgctaat gcctcactgc aatggctgtc
     9241 cgaccactac gaattgtttc ctcacttggt ttctttactt aatccgcagg gcgtgctggc
     9301 agtacagatg ccagataact ggctggagcc gacccatgtg ctcatgcgcg aagttgcctg
     9361 ggaacaaaac tacccggatc gcgggcgtga gccgttggct ggcgttcatg cttactacga
     9421 tattttgagc gaagccggat gtgaggtcga tatctggcga accacctact atcaccagat
     9481 gccgtcgcac caggcgatta tcgattgggt gaccgccact ggattacgtc cgtggttaca
     9541 ggatctgacc gagagcgaac agcaactttt tcttacgcgc taccatcaga tgctggaaga
     9601 gcagtatcca ctgcaagaga acggacagat actgctggca tttccgcgtc tgtttattgt
     9661 tgcccggcgt atggagtaaa tatcacgtca gctggtaatg acgatcggga agaatttttg
     9721 ctggaatttc ggcttcatcg ttcatctgta agaggtcaat ttcaatagcg ttacagatgg
     9781 catccagtgg taaatcattg ttttcaatac cgaacggatc ttccagttct tccgccagac
     9841 aatccagcga aataaaagtg taggaaatca gcacagaaat aaaaggcgtc atgtaatgca
     9901 ggtccacgac cagcgcgaac ggcagcatga tacaaaacag ataaacggta cgatgcaaaa
     9961 tcagggtata ggcaaaggga attggcgtgt aggcaatgcg ctcgcagcct gccagaaccg
    10021 ctgaaatatc gttaagccga tcgttgaggc taataaacag gatatctgaa agctgtccat
    10081 tgcggcgctg aaccgccaac cattctccca ttattaacaa gatacggtta gctggagagt
    10141 tcgaagccag tacacgctga agatcttcag tcttgagata atgagccagc acttccgcct
    10201 gtggctgttt gcgtaatgtc atgcgtaaac agtgggcgaa ggcgatttgc agccgggcaa
    10261 actcccttac acttgccgaa tccggcaatg tcgtttttac ttcgcgcagt aacgaccgcg
    10321 aggcaatcat caactgcccc caaagtttgc gcgcttcaac gtaacgggcg taaccggcat
    10381 tattacgaaa accaagaaaa atggcgatgg cgacaccgag aatgctgaac ggtgcgaggg
    10441 tgaatttgat gcccagatgc gtgtaccagg gcagcatgaa aataacagcg atagaaaaga
    10501 gaaaattgag tagtaagcgc gaggatatct tggataatac tgagccatgc cagacaaaaa
    10561 tacggcgcag ccagtgttgt tgtggacgaa caatcatggt tatcttcagg cgtggaaaag
    10621 tcgccctatt aaacgtgatt acgatcacat tctcaagacg ctacttacaa attacctaca
    10681 aaattacaac attaacaggc gctgcatgtg gcacctgtta atgataaagg cttataggat
    10741 gttgtaacta atataatgaa ataagaaacc cggtcgaaac cgggttcaga gtattagcac
    10801 aacggacgta cagcttcgcg catccctttt tcgagaatcg catccaggtc attagccacc
    10861 tgctcgacca gaccaggcac ttgcgtcagg tcctgctccc agtggtcttt ttctgccaac
    10921 acaatcgcta ccagttcctg ggtaccgata acacggtcac gatgctgact ccagagttgc
    10981 tgataacgtt ccagccagtg tgcatcgtcc tgtaccggat atgtttctcc attacgctca
    11041 ccgcgataga acgcaatcaa tgccgctaat gcgaaagtaa ggcgcgccgg aagtgtgccg
    11101 tttgccttct gccctgccag cagctgcggc aggatgcggg tacggaactt ggtcataccg
    11161 ttgagcgcga tagacagcag ctgatgctta atgtacgggt tacggaaacg cccggtgact
    11221 gcactggcga aagactccag ttcatcgcga ggcaaatcca gaaccggaat aatttcttcg
    11281 tagatggctt tttcaacgaa tgcgcaaatt tcagcatcgt tcatcgcttc acctacggta
    11341 tccagccctg cctggaacgc caccggcacc agcgcggtgt gagcaccgtt gaggattgcc
    11401 actttgcgtt ctttatacgg tttaatatcg tcaacaatca gcacgttgag cggatatttg
    11461 tccaggcgca gctcagtcgc taaggatttc ggaccctgaa tcacaaacag gtaaaagtgt
    11521 tcagcggtgt cgaggaaact atcgtgataa cccagttctt cttccagttt agccacttca
    11581 tcacgcggat aaccggtaac gatacggtcc accagcgtag aacagaagct gttagcctga
    11641 tccagccatt gaataaatgc ttctggcagt gcccactctt gcgcatagcg cagcaccagt
    11701 tcacgcaacg cgtcgccatt gtagtcgatc aactcacacg ggatgatgat ccaaccttta
    11761 tccagcgcac cgttgaaatg gctaaaacgt tcgaacagca gacgggtcat ttttgccgga
    11821 taacttaccg ctggcgcgtc atcgaattta tcgcccgcat ggtagctgat acccgcttcg
    11881 gtggtgttag agaaaacaaa gcgcatttcc gggttgtgcg ccagtttcag gaattcatcg
    11941 tattcactgt agacgctgat ttcacgatta acagagcgaa tcagacgcgc gtcgctgacc
    12001 gcttccccct tctcattcag gccacggata atggtggtgt acagaccatc ctgcgtgctc
    12061 agtgacggcg ggaatgaagt ttcaatcgga cgaacaacga ccacaccaga attcagatcg
    12121 gtgtgctcat tcaggagatc gatttgccag tcaacaaagg cgcgcaggaa gttaccttca
    12181 ccaaactgaa tgatacgttc tggatactgt gcaccgggaa aatcgcgacg atttagtgtt
    12241 ttcacaatgg gttcccttct gattagtcat acaacctgtt tgaattggta cgacaggtta
    12301 gcaaacttta atacgacgaa cccctgtttt gatcaactcc tgatgattaa tgagcagttt
    12361 tatgagaaaa gtgtggcgcg gatcatggtt taatcgagga aatacgcctt ttcctggatc
    12421 ataaagtggt agaacacatt gcattcaaat cgctctgaat gaataaagat gtcagacaac
    12481 ttcctcaccg taacgcatag tgctggtacg gttgcgccca tctttcttcg agcgatacag
    12541 acacgtatcg gcttcgacca tcaatttatt aaagtcatcg gtcagcgttc ggtacgatgc
    12601 gcgaccacta ccgacgccaa tacttaccgt gagataaagc gttttttgtt gccaggtgaa
    12661 tggttgcagt tcaacgcctt tacggatttt ttccgccatt agcagaccat cgacaggatt
    12721 caccgacggc actgcaacag caaattcttc gccgcccatg cgcgccacca gccccttatc
    12781 accgacaatc ttctgaatat gccgggcaaa cacgcttaac actttatcgc cacattcatg
    12841 tccatagtta tcgttaatac ttttgaaata gtcgatatca agcagcatga cagtcagatg
    12901 ttgtgtctgt ttcagcgatg gacttttcag cgcctcataa agaccggacc gtgagtaaac
    12961 ctgagtcaga aaatcaaagt cggctcgcag cgcaacttgc ttcattagcg aattgatcgc
    13021 tgccacgcta aaagaaacca taattgggca tatcgccatc gtggcaatac cgagacgtgc
    13081 ggagaacatt tgcggaatgg agaacggcga accgaccgag atatcaatca ccgaattagc
    13141 caccagtacg atttccaccg caccggtgac aaaggtcaac agacatgtta cctgcggcgt
    13201 atagcgcact gcacaccaga ttaatgcagg cagcgggaac gccagactcc ccgctccgcc
    13261 aatgactacc gaggcaataa ccgacacaat aagcgcgatg gcgggcatca tctgttctgc
    13321 tttaaagcgc ggcagcactc caggaatagc caacgtcagc atacaaggta cgatcaacac
    13381 gcccgttgag aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc
    13441 aatactgacc gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta
    13501 attaaatagc cgtaaggcac ttactggctc atatttattt ttccccaaac gcttatcccg
    13561 cacgaccagt aaggcgacgg taacaataaa catcatattg gagaaattga tagccagtga
    13621 aaccagtccc cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc
    13681 atagtaatgc agtcgattaa gccagacata gcgggcaaac acccccgcca ttacgccatt
    13741 cagcggccag aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa
    13801 cagcgtggtt agcgcaaaga tagcgatgct attacgtagc agatgacctt cctggaacac
    13861 acggaaagtt gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc
    13921 aattatctca acgtaataag taaattctca taaaggatag catgttcgct gacagcggcg
    13981 gcaaataaag gtaccctggg aatgctattt tgtcgttttc cgcaattctc atggctgatt
    14041 catctgtaca accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct
    14101 tagcgaaaca ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc
    14161 ggtggcgtgc tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt
    14221 agccggtaat gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt gttcgatgac
    14281 tggcgcgcct ttctccagac aatactgacg ctctttcatg cccaaacgaa agcgagatcg
    14341 aaattttgaa cgggataaac gcgcgaagag tgtatcgaga gactgcatta ataaaccgaa
    14401 cgccctaact gtttcgtcaa ttgttcaagg acggcgatac ccgcaagcga gttacctgca
    14461 tcatccagtt ccggactcca gactgcgatg gccatttcat gcggaacaat cgccacaata
    14521 ccgccaccaa cgccagattt cgccggtagt cccacccgcc aggcaaactc ccccgcgttc
    14581 tggtacatac cactggtcgc catcagcgcg ttaatttgcc gtgcctgcat tggcgtcacc
    14641 acaggttcat caatatgaat agctttcccc tgattagcca gaaagacaaa cgtccgggcc
    14701 agctctacac agctcatttt cagagcgcag taatgaaaat agttttgcag aacggttgtc
    14761 acgtcatgat ggaaattgcc aaacgacttc atcagccagg cgatagccgc atttcgcgcg
    14821 gaatgttcaa attcggaacg cgctaccacc gtatcgtagg aaatatcaga cacaccgctt
    14881 aagccgcgca cgacttccag catacgttgc cgtggtgcgc ttaatcgccc ttgcaacata
    14941 tcgcagacca ccagcgcacc agcattaata aacggattac gcggaatacc ctgctccatt
    15001 tccagttgca ctaaagaatt gaacggtgat ccagacggat ctttgccgac gcgttgccag
    15061 atttcctctt cggagtaatg acgcatggcg acaacgagac tcagcacttt ggaaatagac
    15121 tgaatggaaa aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag
    15181 atagcaatgc ccaatcggga accgtctact gtagccagcg ccggaatata atccgcgact
    15241 ttaccctgac caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc
    15301 atggcgactg ccactttcta ctcctggacc gcaggtctga aaagacctgc gagtatatca
    15361 gagctgaata tgtcgcgtca gatccggtct ttccacaccg tctggatatt acagaattcg
    15421 tgtaagccga aatgggaaag ctcacgacca aagccactct ttttcacgcc accaaaggcc
    15481 actcgcgcat cgctggcaca ataaccattg ataaacaccc caccgcattc cagacgtgcc
    15541 gccatctgtc tggcctgtgt ttcgtcggtg gtaaaaatgg tcgctgaaag gccgaactca
    15601 ctatcattag ccagttccag cgcatgttct gcatctttcg caacggtgat tgccgcaacg
    15661 gggccaaaca tttcttcccg aaacgcggtc atttctgggg taacattcgc cagaaccgtt
    15721 ggcggatagt agttacctgc cccagccatc ttttccccgc ccagtaacaa acgcgcaccc
    15781 tgcgccaggg ttttctccac ctgatgatgc agctcatcac gtaaatcgaa acgagccatt
    15841 ggtccgagag cgttctcttc gtcacgggga tcgcccattt tcaaggctgc cgcagctgcc
    15901 acaaaacgtt cggtaaatgc cgaagcaatt ccctcttcga taataaagcg ttttgccgct
    15961 gcacaaacct gtccggtatt ctgataacgt ccggctaccg ccgctttaac cgccagttcc
    16021 agatcggcat cgttaagcac aataaatgga tccgaaccgc ccagttccag tacgcatttt
    16081 ttcagtgccg ctccagcctg tgcaccaata gccgctcccg cacgaacact tccggtcacc
    16141 gtgacagcag caatgcgcga gtctttaatc atctgactga caccgtcgtt gtgggcattc
    16201 agccagccat atacgccttg tgggattccc gcatctttaa acacctgggc aatgagctgt
    16261 gcacagccca tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg
    16321 ggaacagcgc cacgcatcac ctgccataac ggaaaattcc acggcataat cgccagaatc
    16381 gtccccaacg gtcgatactc aataactgcc tgctgatttt ccaccagcgt aggttccgcc
    16441 ttcagcattg ccggaccatg ttctgcatac cagtcacaca aattcgccga tttcgccact
    16501 tcagcgcgcg cctggttgat tggtttgccc atttcgcggg tgatcatttg cgccatttct
    16561 tcgctacggg cgcgcagagc cttaccgata tcacgcagtt tttcagcacg ataatctata
    16621 tttgtctcgc gccagtcacg aaagcctgct gccgccagct gaagtgcgtt ttcgatatca
    16681 tcagcgccag cccacggcag cacagaaagt tgttcacccg tggcaggatt tatcgaaatt
    16741 gcatgagttg ccggagtaat ggtcatcggg gtatctcctt tatgagtcat ggtatgaaga
    16801 tacgcagatt tactcttgct ttaaaatgaa taatattaag ccacttattc acgaatcgag
    16861 aatattatgg atctgaccca actggagatg ttcaacgcgg ttgccgaagc tggcagcatt
    16921 acgcaggctg cagcaatagt gcatcgcgtg ccgtcgaatc tcactacccg tctacgccag
    16981 ctggaaacag aactgggggt agatctgttt attcgcgaga atcagcgttt acgtctatct
    17041 cctgccgggc ataacttttt acgctatagc caacaaattc tcgcgttagt ggatgaagcg
    17101 cggagcgttg tcgctggcga tgagccgcaa ggtttatttt ctctgggttc gctggaaagc
    17161 accgcagcag ttcgcattcc ggccacgctg gcggaattca accgtcgtta tcccaaaatt
    17221 cagttttcac tttccaccgg cccttccggc actatgctgg aaggtgtgct ggagggaaaa
    17281 cttaatgcgg cgtttattga tggacccatt aaccatactg ccattgacgg gataccggta
    17341 taccgcgagg agctgatgat cgtcacgcca caaggtcatg cgccagtaat ccgtgccagt
    17401 caggttaatg gcagtaacat ttatgccttc cgcgccaatt gttcgtatcg tcgccacttc
    17461 gagagctggt ttcatgctga cggtgccgct ccgggaacta tccatgagat ggagtcttat
    17521 cacggaatgt tggcctgtgt ggtcgcagga gcaggtattg cgcttattcc gcgctctatg
    17581 ctggaaagta tgccggggca tcaccaggtt gaagcgtggc cgttagctga gcaatggcgt
    17641 tggctaacaa cctggctggt ctggcgtcgt ggtgcgaaaa cgcgtccgct cgaggcattt
    17701 attcaactac tggatgtgcc tgactcgaca aaacagggtt accaatgagc tatttttgat
    17761 agttcttgcg gctaatatgc tctatatagt gatgttccga tgacttatga ctatatgggg
    17821 caaatatggt tacgccagta agcatcagca attacatatc tcttcctgat gattttcccg
    17881 ttcgtaatat tgcaccgcag gtaaaagaag ttttaaagga ttttattgat gcacttagta
    17941 caataatatg taatgaagag tggcgcacgt ctttaaacat caactcagcc acaaaaaaga
    18001 tatttaataa ccttgacaac ttatcatata ttcagaggac ttcttttcgg ggtaacgaca
    18061 cgctatacaa tgaaaaggtt cagtttaaac tcactt
//
LOCUS       DAZUCE010000093        18072 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZUCE010000093 DAZUCE010000000
VERSION     DAZUCE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.093"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..2234
                     /locus_tag="ACHY03_002664"
     CDS             <1..2234
                     /locus_tag="ACHY03_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFS3905873.1"
                     /translation="TGNACATEWISSEDLITSDFHLMTADERNVVKAATDDSMEAAYM
                     LKDNIRWYYHNGNLSLPANFSNQNKLVVNGNLTISGDYDDYLSGNGHLIVLGNVIVDN
                     FINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVSDKATQFEVIKAEFYIN
                     EDESGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPDYDSVADNIVQGLPLFR
                     DKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTESLSPAVMLQLLQHPDD
                     QTRESMAQSWPAQQMHLLTDELIKDEAVARGLVKNSNISADVNKKLMSVPVESVQLEQ
                     ARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVADELINNEDPELRERITG
                     ADLTAQQAVMLSKDRSLKVREALARTLTELKITQLSATLRTEDIERIAEQMYLDNKEN
                     KNIVKALLIALPEMRQLSLAKEDVHNLREGARYLTSKDVISYLLTQHDVPTVWDELAR
                     DKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNGIVDEEMLNNAIDLLVD
                     LPAEYRYRMRNQLFDNKDLPSGIINKLDQQYRFNSDWALAVVSMKNSTRRQSERGLHR
                     WNHEDSDIFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASLLTTLTESQD
                     RSLAINNPQLAADVKTVWLNEDPSLLLFVDQPDLSQLRDLVKTGATRKIRNEARHRLE
                     EKQ"
     gene            2231..2686
                     /locus_tag="ACHY03_002665"
     CDS             2231..2686
                     /locus_tag="ACHY03_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905874.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGVVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(2764..3675)
                     /gene="rdgC"
                     /locus_tag="ACHY03_002666"
     CDS             complement(2764..3675)
                     /gene="rdgC"
                     /locus_tag="ACHY03_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFS3905875.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            3800..4708
                     /gene="mak"
                     /locus_tag="ACHY03_002667"
     CDS             3800..4708
                     /gene="mak"
                     /locus_tag="ACHY03_002667"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFS3905876.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(4851..6035)
                     /gene="araJ"
                     /locus_tag="ACHY03_002668"
     CDS             complement(4851..6035)
                     /gene="araJ"
                     /locus_tag="ACHY03_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFS3905877.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(6161..9304)
                     /gene="sbcC"
                     /locus_tag="ACHY03_002669"
     CDS             complement(6161..9304)
                     /gene="sbcC"
                     /locus_tag="ACHY03_002669"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFS3905878.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLTDVKTICEQETRIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLNAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQFLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTARQNRIQQLTPILETLPQSDELPHCEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(9301..10503)
                     /gene="sbcD"
                     /locus_tag="ACHY03_002670"
     CDS             complement(9301..10503)
                     /gene="sbcD"
                     /locus_tag="ACHY03_002670"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFS3905879.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHIRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQTLTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            10693..11382
                     /gene="phoB"
                     /locus_tag="ACHY03_002671"
     CDS             10693..11382
                     /gene="phoB"
                     /locus_tag="ACHY03_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFS3905880.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGGEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            11440..12735
                     /gene="phoR"
                     /locus_tag="ACHY03_002672"
     CDS             11440..12735
                     /gene="phoR"
                     /locus_tag="ACHY03_002672"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFS3905881.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            13142..14461
                     /gene="brnQ"
                     /locus_tag="ACHY03_002673"
     CDS             13142..14461
                     /gene="brnQ"
                     /locus_tag="ACHY03_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFS3905882.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            14537..15910
                     /gene="proY"
                     /locus_tag="ACHY03_002674"
     CDS             14537..15910
                     /gene="proY"
                     /locus_tag="ACHY03_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFS3905883.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            16066..17883
                     /gene="malZ"
                     /locus_tag="ACHY03_002675"
     CDS             16066..17883
                     /gene="malZ"
                     /locus_tag="ACHY03_002675"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFS3905884.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGPQWAADQIFYQILPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         4174 a         4461 c         4824 g         4613 t
ORIGIN      
        1 tgactggcaa tgcatgtgcc acagagtgga tcagtagcga ggaccttatc acctctgact
       61 ttcatcttat gacggcagat gagcgcaacg tggttaaagc ggcaaccgac gatagtatgg
      121 aagctgctta tatgctaaaa gataatatcc gctggtatta tcataatggt aacctatcat
      181 tacccgctaa cttcagcaat caaaataaac tggtggtgaa cggtaattta acgatttccg
      241 gcgattatga tgattatctg tcaggtaatg gtcatctgat tgtattaggt aatgttattg
      301 tcgataactt tattaatcat gatttcgcgt atgtcaaagg acagatgaca gcgaaaggtt
      361 tagtttatgc tgattacaat gatcacaact ttgaagtgat gaagggaatt agcgcgcgag
      421 gcattattgt ttccgataaa gcaacgcaat ttgaggtcat caaagctgag ttttatatca
      481 atgaagatga aagcggtgaa ggatataact gggatgagaa tattcaaaaa gcctattcac
      541 tggtgactgc cgatctttat gatcacactg aaatcgaaac cgataacatt tccaatgcgt
      601 atcctgacta tgatagcgtc gcagacaaca tcgttcaggg tttaccgctt tttcgagata
      661 aagccgctcc tgaaattaat gagaaattaa aatggattga aacggggaaa ctcgataatt
      721 tcccagcgaa taaaataaag catcaagatc cactggtggc acgttttctg actcatacag
      781 agagtttatc acccgccgtt atgctgcaac tgttacagca ccccgatgac cagacgcgtg
      841 aatcgatggc gcagtcctgg cccgctcaac agatgcattt gcttaccgat gaattaatta
      901 aagatgaggc tgttgccaga gggctggtta aaaacagtaa tatttccgcc gatgtgaata
      961 aaaaattaat gtctgtaccc gtcgagagcg tacagctgga gcaggccaga caggataatc
     1021 tttctccaga tattgttgcg tcgttaagtc atagcccctt tctcagtgta cgcaagacat
     1081 tactcagcca ttacgattac gcctggctgg ttccgactgc agtggccgat gagttgataa
     1141 ataacgaaga cccggagtta cgtgaaagaa ttaccggagc agatttaacc gcacaacaag
     1201 cagtgatgtt gagtaaagat agatcactga aagttcgtga agcattagcc cggacgctta
     1261 ctgaattaaa aattacccaa ctgtcagcaa ccctgcgtac agaggatatt gaacgtatcg
     1321 cagaacaaat gtatctggat aataaagaaa ataaaaatat cgtaaaggcg ttattgatag
     1381 cgttgccgga gatgcgtcaa ctgtctctgg caaaagaaga tgtgcacaat ttgcgcgaag
     1441 gtgcgcgtta tctgacatcg aaggacgtga ttagttatct attgactcag catgatgttc
     1501 ccaccgtctg ggatgagctg gcacgcgata aattattgcc gctggaatat aagaaacagt
     1561 tatggcaacg cacattaaat ttaatgatga gcaaacggca agaagatcaa gagcaagcct
     1621 acgaagtaca gctggcactg attgataatg gtattgttga cgaagagatg ttaaataatg
     1681 ccattgattt actggttgat ttacccgctg aataccgcta tcgaatgcgt aaccaattat
     1741 ttgataataa agatttacct tccggaatta tcaacaaact cgatcaacag tatcgcttca
     1801 actctgactg ggcgctggct gttgtcagta tgaaaaatag cacccgtaga caaagtgaaa
     1861 gaggattaca ccgctggaac catgaggata gcgatatttt tgcggaactg gcgacgataa
     1921 aagataaatc agatgatgaa tggtggcgtg cattgctgca aagtcgcaat gatcatttgc
     1981 gccaaacggc attacgcaac gcacataccc cggcgtcgtt gttaacgaca ttgactgagt
     2041 ctcaggatcg gtcgctggct attaataatc cacagctggc tgccgatgtg aaaacggtgt
     2101 ggctaaatga ggatccatca ttacttttat ttgtcgatca acccgatctt tcgcagttac
     2161 gggatttagt gaaaaccggg gcaacgcgga aaattcgcaa tgaagcgcgt caccggcttg
     2221 aggaaaaaca atgacacaac gcccctggag caagttacag cgggagatct atgacttact
     2281 gacgccaacg atcaacctgc aaattcactg tacccgttat cccatgcgca gccaaaatgg
     2341 tggaagcacc gatttaccgc gttattggat cacgttggat aaaaacgtga tctgggatta
     2401 cccgaaagac tttattgcag gtaacggtgg agtgcgtaat tttcacggtg aaacttgctg
     2461 gtatccttac ctgacggata tttgttcaat ttctgattta ttgcgtgaat atattgacac
     2521 cccaaaagcg gagttgttaa caaaacaatt taccagcgat aaatgggggg tagtgaatat
     2581 tctgcgtgct gctgacaggc gaatagggat gaggcgatta gatcagttac ggcgtaaaac
     2641 gcataatatt gctgcgctaa aaattattgc ccgccgtagc gaataattat atgcctggtg
     2701 tggcttcgta cgccggataa gacgcggcag gcgtcgcatc cggcattaaa ggaaaatcag
     2761 caattaacgt tgtgcttcgc cacctaatcc ttcaatcagg ttttgaatta acgctgccag
     2821 ttcaccagtc atcaggatga aatcggcatc aaaacgctgg gcgaaatctt cacggtcgat
     2881 atcttcgttt tgatcgcgca gctcgtcgca gaacttcaga cgcttgagcg aaccatcgtc
     2941 gcacatcaca aactgaatgc gctgctgcca gtcgagcgcc agtttagtca ccacttttcc
     3001 ggcttcaatg tgattggtga tctcttcgct ggtcagatct tgtttcttcg cgcggatcac
     3061 tccgccatct tccagcaacg atttcagctc ggcttcatca agcagctgga agccctgtgc
     3121 cgcactaccg gaacgtaccc attcggtcag cgtcagttca atcgggtttt ccatgctcaa
     3181 tggtacaacc ggtaacgacc ccaggctttt acgcagcagt gccagcgtat cttccgcttt
     3241 tttggcactg gcgcagtcca ccataatcaa accgttaacc gtgtcgatcc acatcattgt
     3301 ctggctaaaa cggctgaaag cacgcggcaa cagagagtgc agcacttcgt ctttcagcga
     3361 atctttttcg gttttcttca gcttacgcgc ctgttccgct tccagtttgg cgattttcgc
     3421 ttccagcgcc tgtttaatca ccggagacgg gaggattttt tcttctttgc gtgcgcagat
     3481 aacaatttga ccattggcaa cgtgcgttaa cgcatcgctg tgcgatccca tcggaggaac
     3541 ccagcccatc ttcgccatgt cctggctgcc gcatggggta aatgccatcg aggctagctg
     3601 tttttccatc tcttctgcac gcagcgaaat ctcgcggcta agacggtaaa ccattaaatt
     3661 tttgaaccac agcatgataa tttccacggc cttgtcgtta aatttagcgg gcatgataac
     3721 gaattgtcgg cggccttgca ttgccaatcc ggttgtccgt ctctacgcta ttgatattga
     3781 aaaaaataag gagagtaccg tgcgtatagg tatcgattta ggcggcacca aaactgaagt
     3841 gattgcactg ggcgatgcag gggagcagtt gtaccgccat cgtctgccca cgccgcgtga
     3901 tgattaccgg cagactattg aaacgatcgc cacgttggtt gatatggcgg agcaggcgac
     3961 ggggcagcgc ggaacggtag gtatgggcat tcctggctca atttcgcctt acaccggtgt
     4021 ggtgaagaat gccaattcaa cctggctcaa cggtcagcca ttcgataaag acttaagcgc
     4081 gaggttgcag cgggaagtgc ggctggcaaa tgacgccaac tgtctggcgg tttcagaagc
     4141 agtagatggc gcggcagcgg gagcgcagac ggtatttgcc gtgattatcg gcacgggatg
     4201 cggcgcgggc gtggcattca atgggcgggc gcatatcggc ggcaatggca cggcaggtga
     4261 gtggggacac aatccgctac cgtggatgga cgaagacgaa ctgcgttatc gcgaggaagt
     4321 cccttgttat tgcggtaaac aaggttgtat tgaaaccttt atttcgggca cgggattcgc
     4381 gacggattat cgtcgtttga gcggacatgc gctgaaaggc agtgaaatta tccgcctggt
     4441 tgaagaaagc gatccggtag cggaactggc attgcgtcgc tacgagctgc ggctggcaaa
     4501 atcgctggca catgtcgtga atattctcga tccggatgtg attgtcctgg gaggcgggat
     4561 gagcaatgta gaccgtttat atcaaacggt tccgcagttg attaaacaat ttgtcttcgg
     4621 cggcgaatgt gaaacgccgg tgcgtaaggc gaagcacggt gattccagcg gcgtacgcgg
     4681 cgctgcgtgg ttatggccac aagagtaaaa aacgtaggca attggcgcat catgcctgat
     4741 gcgacgcttg ccgcgtctta tcaggcctac aaaatgtgcc agaaccgcgt agggcggata
     4801 aggcgttcac gccgcatccg gcaataagca acgtgctccg tgactgcaat ctaccccaga
     4861 ggtttcgcca gcaccggact atccgccgct tgctggcgct tatagcgacc atacagcagc
     4921 aatgacgaca tcgcagcaaa cgaaagcagg gcggcaggca gcgccacgta attatatgcc
     4981 agccccagcg tcagcatcat accaccgcaa tatgcgccga cggcgctacc gaggttaaag
     5041 gctatttgcc cacctgcggc acctaataac tctccgcctt tggcgttttg cagtaacaat
     5101 atttgcagag gtgctgaaag ggcaaataat cccgcgcaac aaataaaagc aaaaataagc
     5161 gacgttgttt tcataccgcc gcagaaaaag agcatcagca gtgccagtac aattataaag
     5221 tcagtcactg ctgcaatgcg cagtggtgaa taacgtcctg aaatcctgcc acttagcata
     5281 tttcccagca ccatccctag cccaactaac atcataataa aggtcatcgc cgtttccgaa
     5341 aaaccggaaa taaacatcat gtatggcttt acgtagctga accaggcaaa cacacctgcg
     5401 ttgccaaaca tggtggcggc gaaaattaac cacggggccg ggctgcgcaa aaagtgaaat
     5461 tgttcgcgca gctttccttt cgcctcgtcg cgaatatccg gcacccaaaa atagaccgat
     5521 gccatcaccg caatattaaa aacagcgatc aataaaaagg tgtaacgcca gctaaattcc
     5581 tgacttaaat acgttcccag cggaatgccc agcaaattgg cgactgtcat cccggaaacc
     5641 atccccgcca cggcggcggt gacttttccg ggtttgataa tttttgataa cacgatcgct
     5701 ccgacgccaa aaaatgcgcc atgcggaaag ccggaaacca ggcgaccaat ggcaagcatc
     5761 aggtaagacg aagagagcgt gaacatggcg ttgccaatga cgcacaacgc caccagaaac
     5821 aacaagatat gtttgagtga gtagcggctg gaaaagagtg cgattattgg cgcaccgacc
     5881 accaccccca gcgcataata cgagatcata tgcccggcag caggaatcga aattcctacg
     5941 ttatgcgcca gctccgtgag cacgcccata atgccaaatt cggccatccc caaaccaaac
     6001 gtgcccagag ccaaagataa aatgactttt ttcataccac catcacaacc accagaacgg
     6061 ctaccaatca ccgtagccaa taaagtgata gcttgcagga taacgaccag caaggccaag
     6121 agataattcg cccctctgta ttcattatcc tgctgaatag ttatttcact gcaaacgtac
     6181 tttccagttt gctgtagccc aggccgttga tctttttcac tttgatctgc accggaatac
     6241 gctctttcat cgcttctacg tggctaatca caccgatggt tttgccactg gcgttcaggg
     6301 catccagcgc atcaagggcg gtatccagcg tttcgctatc cagcgtgcca aaaccttcat
     6361 caaggaacag cgagtcaata cgcgttttat ggctgaccag atctgaaagc gccagcgcca
     6421 gcgccagact gacgaggaaa ctttcgccgc cagaaagggt acgggtgtcg cgtaccgcat
     6481 ctgcctgcca ggtatcaaca acctcgactt ccagcgcctc gctggctttg cgctgtaaca
     6541 gatagcgccc gtgcagccgg gtaagttgct gattagcgag atggactaaa ttatccagcg
     6601 tcagcccctg ggcaaatttg cggaatttat cgccctcttt ggaacctatt agcgaattca
     6661 aatatcccca gtcctcaacc tgctgcgtca tttgagcaat ttgctgcatt aaggtttgtt
     6721 gttgctgacg gttatctgca tcctgcttca gctgctggcg aatctcgcct tgactcgtgg
     6781 tgttttcacg caacttttgg tgagtttgcg ctaactcttg ctgaatctgc tccaccgtca
     6841 cagtgagagc caacccgtca ggtcggtgtt gctgatgctg tgccagcgtt tctgctgtct
     6901 gagtgaccag cgtttgcgcc tgacggcgct ggttttccag attctgcttg agctgttcca
     6961 gctgcgttag tgtttgttca tccattagcg ccgcaaggaa cgcctgctga tcgtcaaaga
     7021 cgctggcctg tagcgcggtg tcaaactgcg cctgggcttt ttgcagactt tgcgccgcca
     7081 gaacgtcctg ttgctgtaac gtctgctgct ggctgtgtaa tgcgagacat tgttcatgta
     7141 cctgccgcca gttttccagt accacagttt cttcgcagtg cgggagttca tcactttgcg
     7201 gcaacgtttc cagaatcggc gtcagctgct gaatacggtt ttgccgcgcg gttaattcgt
     7261 tctggcgttg ctgccagctc tgcgcttctt gctgacgtgt cgccaaccag ctctcttctt
     7321 catcttcctg tggcaatgtc agtgcataac ccgccaatgc cgttaagaac tgctgctggc
     7381 gttgttcaat ttgctgttga tactggataa tttgctgatt atgcgcggca atctgccctt
     7441 gtaattcatg tcgttggctg agtaaccgca gctggcgttc gtgctcatct tgtgcattta
     7501 gccacggttg aatatcgtcc tgtggctgca aggtgatatt gaggctggcc gtgacggctt
     7561 gccattgttg agtaagtgct tgctcatctt gtcggaggct ttgcgcttcg ttttcatcac
     7621 gctgaagctg ctttgttaag gcgtccagtt gcccacgtag cgccgcacct tcttcaccga
     7681 gctttttaac ttcgttttcc agcgccagta atcgagactg attaacgcca ggctccagcg
     7741 cctgatacgc ctcgaccgcc gggtggctgg tggaaccaca aagtggacaa ggctgacccg
     7801 cctgtaactg tgcgcgctgg gcttccagcg ttttgatgcg cgtttcctgc tcgcaaatgg
     7861 ttttcacatc ggtaagttgc tgcgtctttt ctttataacg ctggcgcatt tcgttaagtg
     7921 cggcattacg ttgcgtctgt tccagagtga cattctggat agcgacctgt aactgcgcca
     7981 gacgtttttg ttggggaaca atctgcccat gcagcgcgac caggcgctga cgcagtgggc
     8041 gttgctcggc atgttgcgcc agggcgctag caacttcatc ggcggttaac gtcaacgtga
     8101 tcgccgcaag cgcattaagt ttttgctctg catgggttaa ctgttgctgc cattgccgca
     8161 gatgctcgcg atcgctggtt tgttgggaga actgcgcacg ccaacccgcc agttcgttgt
     8221 tccactgacg gaagcggtcg tgttcctgta gccaggcatt cagactttgt tgctgctgct
     8281 gtaattctgc tgactgcttc gccgcgtggt ggcgaatgct cgcgcgaagc gccattgtgc
     8341 tctgtaagcg agtatttact tcttcaatct gctggcgagt atgcgccagc gccgcgctgt
     8401 gttctgcgat gcgttcccag tgtggacgaa gatttcgtgc cggttgtgcc agactgagcg
     8461 ccgccagttg aggttgcgct ttttcttctt cggctaacgc ctgttgcaag gcctgttgac
     8521 ggcggctggc ttcttgctgc aattcgtcca gacgcgttaa ccagtttagt gattgttgtt
     8581 cttgctgctg cgcggtaagt aactgttttt cttcgtcagt aagtacctgc aaactcgctg
     8641 tcagcgattg cacttgttcc ggcgtgagca acgcgacgcc gctggcctgc gcttgcagct
     8701 tctccagctc agtgcgggcc gatttgtgct gctcaaaaac catcgccgag atttgcccgt
     8761 agatttcagt gccggttaac tcctcgagca attccgcgcg ttctttgggt ttggcattca
     8821 ggaaggcagc aaattgcccc tgcgaaagca gcatcgaacg ggtgaagcgc ccgtaatcca
     8881 gtccggttaa cgtcgccgtc agttccagct tatctttcac tttgtcggcg agaattttgc
     8941 catcggcgca gcgcgccagc tctacgcgtg gcacctgcaa attaccgtcg ggttggttac
     9001 gcgcccgatt ctggctccag aacgcgcgat acgcttcacc tttcacttca aactccacct
     9061 ccgccagaca ttcggcggta tcgcgggtca tgagatcatt ttgcgattgt gaaacgttag
     9121 agagacgcgg agtttcgtga tacagcgcca gacaaatggc gtccagcagg gtggttttcc
     9181 ccgcaccggt tgggccggta atggcaaaca gcccgttgct ggcgaacggc tcgcgggtga
     9241 aatcaatctt ccattcgcct tttaatgagt tcaggttttt caggcgcagg ctgagaattt
     9301 tcatgcttcg tgttctccgg cgagggtatg caacgtcgtg gtgaaaagat gctgcagacg
     9361 ttgctgctgc gactcatcca gttcttccag tgccagacgg cgattgaaca cctcttcgac
     9421 gctgagttcg ctgagggttt cacgctgttg gctggctaac acacgctcgc gctgttcacg
     9481 actccggcgt accagtaata cttcaacagg caatgattcg gttaatgtct ggattttgcg
     9541 ctgaatatca tgcagatact catcagtagt gatttcgata tccagccaga caggcggctc
     9601 ctgcgataca tcgcgccact gttccagctg tgcggtaatc gacgccagat cgcctttcag
     9661 cactgccatg ggttgcgtta ccggtacgtt caggttttcc acgctctcta atttgccgtt
     9721 tgaaaatgtc accagatgga catatttact ctttccgcat tcatcaaaac tcagtggaat
     9781 gggggagccg caatagcgaa tatgttccat gccgccaata atctgtgcgc ggtgaatatg
     9841 cccgagcgcg atgtagtcgg ctggtggaaa gttttgtgcc ggaaacgcgt ccagcgtgcc
     9901 aatataaatg tcacgcacgg cgtcactttt actggccccc acggtcgtta aatgtcccgt
     9961 ggcgatgatg ggcagaggct gatcgccgcg cagtttgcag gcatcggcat agtgttgttg
    10021 ataataatca gtaatagctg ccagcaaatg ctgctgtttt tcaattccgt taagccccgc
    10081 ctggctggta ataatgtcac gcggacgtaa aaacggtatg ggacacagca ctgcgcctgg
    10141 cgtcccgtcg cgacgaggca agatttgcgg cgcatgtccg gcgctggcga ccacggtcgt
    10201 attgaggaac gccatgatat cgcgcgattc attcagcgtg gcgaccgagt catggtttcc
    10261 tgccagtacc accagatgac agccagtttg ctgtaaattg acaacaaaac ggttgtataa
    10321 cgtgcgggcg taactgggcg gcgagccggt atcgaaaaca tcaccggcaa caataatcgc
    10381 atccacctga tgggtttgtg ctgtctccag cagccagtca agaaaagcct gatgttcagc
    10441 ttcgcggctt ttactataga agttctggcc gagatgccag tctgaggtgt gaaggatgcg
    10501 cataacggtt ccctggcgaa aaagcatggg cgcgattata cccaaacaga tgtgccattt
    10561 gcttttttct gcgccacgga aatcaataac ctgaagatat gtgcgacgag cttttcataa
    10621 atctgtcata aatctgacgc ataatgacgt cgcattaatg atcgcaacct atttattaca
    10681 acagggcaaa tcatggcgag acgtattctg gtcgtagaag atgaagctcc aattcgcgaa
    10741 atggtctgct tcgtgctcga acaaaatggc tttcagccgg tcgaagcgga agattatgac
    10801 agtgctgtga atcaactgaa tgaaccctgg ccggatttaa ttctccttga ctggatgtta
    10861 cctggcggct ccggtatcca gttcatcaaa cacctcaagc gcgagtcgat gacccgggat
    10921 attccagtgg tgatgttgac cgccagaggg gaagaagaag atcgcgtgcg cggccttgaa
    10981 accggcgcgg atgactatat caccaagccg ttttcgccga aggagctggt ggcgcgaatc
    11041 aaagcggtaa tgcgccgtat ttcgccaatg gcggtggaag aggtgattga gatgcaggga
    11101 ttaagtctcg acccgacatc tcaccgggtg atggcgggcg gagagccgct ggagatgggg
    11161 ccgacagaat ttaaactgct gcactttttt atgacgcatc ctgagcgcgt gtacagccgc
    11221 gagcagctgt taaaccacgt ctggggaact aacgtgtatg tggaagaccg cacggtcgat
    11281 gtccacattc gtcgcctgcg taaagcactg gagcccggcg ggcatgaccg catggtgcag
    11341 accgtgcgcg gtacaggata tcgtttttca acccgctttt aacgccttgc tcatcggacg
    11401 cagagcaggg cttatgattt cttaactgga gtatcttacg tgctggaacg gctgtcgtgg
    11461 aaaaggctgg tgctggagct gctactttgc tgcctcccgg ctttcatcct gggtgcattt
    11521 tttggttacc tgccctggtt tttgctggca tcggtaacgg gactgcttat ctggcatttc
    11581 tggaatttat tgcgcctttc atggtggctg tgggtggatc gtagtatgac cccgccaccg
    11641 gggcgtggta gctgggaacc gctactatac ggcttacacc agatgcagct gcgaaataaa
    11701 aaacgccgcc gtgaactggg caatctgatt aaacgctttc gtagcggcgc ggagtcgctg
    11761 cccgacgcag tggtgctgac cacggaagag ggcggtattt tctggtgtaa cggtctggcg
    11821 caacagattc ttggtttgcg ctggccggaa gataacgggc agaacatcct taacctgctg
    11881 cgttacccgg agtttacgca atatctgaaa acgcgtgatt tttctcgccc gctcaatctg
    11941 gtgctcaaca ccgggcggca tctggaaatt cgcgtcatgc cttataccca caaacagttg
    12001 ctgatggtgg cgcgtgatgt cacgcaaatg catcaactgg aaggggcgcg gcgcaacttt
    12061 ttcgccaacg taagccatga gttacgcacg ccattgaccg tgttacaggg ttacctggag
    12121 atgatggatg agcaaccgtt ggaaggcgcg gtacgtgaaa aagcgttgca caccatgcgc
    12181 gagcagaccc agcggatgga agggctggtg aagcaattgc tgacgctgtc gaaaattgaa
    12241 gccgcgccga cgcatttgct caatgaaaag gttgatgtgc cgatgatgct gcgcgttgtt
    12301 gagcgcgagg ctcagactct gagtcagaaa aaacagacat ttacctttga gatagataac
    12361 ggcctcaagg tgtctggcaa cgaagatcag ctacgcagtg cgatttcgaa cctggtctat
    12421 aacgccgtga atcatacgcc ggaaggcacg catatcaccg tacgctggca gcgagtgccg
    12481 cacggtgccg aatttagcgt tgaagataac ggaccgggca ttgcaccgga gcatattccg
    12541 cgcctgaccg agcgttttta tcgcgttgat aaagcgcgtt cccggcaaac cggcggtagc
    12601 ggattagggt tagcgatcgt gaaacatgcg gtgaatcatc acgaaagtcg cctgaatatt
    12661 gagagtacag taggaaaagg aacacgtttc agttttgtta tcccggaacg tttaattgcc
    12721 aaaaacagcg attaatccgc ctttgtcatc ttttattgcc ataagccagc cgatgctggc
    12781 ttattttctt tgcagtcaaa atacgggcgc tagattttac aacggttggt gattttttgt
    12841 tcgcatgatt agccatgtct ttttcacgga aatagtgttt tatactggtt ggtgatttct
    12901 tatcgctata tacctctggt ttttagatcc ctccttgctt taaaacgtta taagcgttta
    12961 aattgcgctt caggtgctgt catactgact gcattcacgc ggtaaatcga aaaactattc
    13021 ttcgccgcgc ctggttggga gtatttcccg ctaaaattgt ttaaatatac cgctgtatca
    13081 tccccaggga ttggcacaaa aatttaacgt tacaacacca catccacagg cagtatgatt
    13141 tatgacccat caattaagat cgcgcgatat catcgctctg ggctttatga catttgcgtt
    13201 gttcgtcggc gcaggtaaca ttattttccc tccaatggtc ggcttacagg caggcgaaca
    13261 cgtctggact gcggcattcg gcttccttat tactgccgtt ggtctgccgg tattaacggt
    13321 agtggcgctg gcaaaagttg gcggcggtgt tgacagcctc agcacgccaa tcggtaaagt
    13381 cgctggcgtg ctgctggcaa cggtttgtta cctggctgtg gggccgcttt tcgctacgcc
    13441 gcgtacagct accgtttctt ttgaagtggg cattgcgccg ctgacgggtg attccgcact
    13501 gccgctgttt atctacagcc tggtttattt cgctatcgtt attctggttt ctctctatcc
    13561 gggcaaactg ctggataccg tgggcaactt ccttgcgccg ctgaaaatta tcgcgctggt
    13621 tatcctgtcc gttgccgcga ttgtctggcc ggcgggttct atcagcacgg cgactgaggc
    13681 ttatcaaaac gctgcgtttt ctaacggctt cgttaacggc tatctgacca tggatacgct
    13741 gggcgcaatg gtgtttggta tcgttattgt taacgcggcg cgttctcgtg gcgttaccga
    13801 agcgcgtctg ctgacccgtt ataccgtctg ggctggcctg atggcgggtg ttggtctgac
    13861 tctgctgtac ctggcgctgt tccgtctggg ttcagacagc gcgtcgctgg tcgatcagtc
    13921 tgcaaacggc gctgctattc tgcatgctta cgttcagcac acctttggcg gcggcggtag
    13981 cttcctgctg gcggcgttaa tcttcatcgc ctgcctggta acggcagttg gcctgacctg
    14041 tgcttgtgca gaattctttg cccagtacgt accgctctct tatcgtacgc tggtgtttat
    14101 cctcggcggc ttctcgatgg tggtttctaa cctcggctta agccagctga tccagatctc
    14161 cgtaccggtg ctgaccgcta tttatccgcc gtgtatcgca ctggttgtat taagttttac
    14221 acgctcatgg tggcataatt cgtcccgcgt gattgctccg ccgatgttta tcagcctgct
    14281 ttttggtatt ctcgacggga tcaaagcatc tgcattcagc gatatcttac cgtcctgggc
    14341 gcagcgttta ccgctggccg aacaaggtct ggcgtggtta atgccaacag tggtgatggt
    14401 ggttctggcc attatctggg atcgcgcggc aggtcgtcag gtgacctcca gcgctcacta
    14461 aatcactgaa catttgtttt aaccacgggg ctgcgatgcc ccgtggtttt ttattgtgtt
    14521 gatgggttag gaattgatgg aaagtaagaa caagctaaag cgtgggctaa gtacccgcca
    14581 catacgcttt atggcactgg gttcagcaat tggcaccggg ctgttttacg gttcggcaga
    14641 cgccatcaaa atggccggtc cgagcgtgtt gttggcctat attatcggtg gtatcgcggc
    14701 gtatatcatt atgcgtgcgc tgggggaaat gtcggtacat aacccggccg ccagctcttt
    14761 ctcgcgttat gcgcaggaaa acctcggccc gctggcaggt tacattaccg gctggaccta
    14821 ctgctttgaa atccttattg tcgccatcgc cgatgtgacc gcttttggta tctatatggg
    14881 tgtctggttc ccgacggtgc cgcactggat ttgggtactg agcgtggtgc tgatcatttg
    14941 cgccgtaaac ctgatgagcg tgaaggtatt cggtgagctg gaattctggt tctcgttctt
    15001 taaagtcgcc actatcatca tcatgattgt cgccggtttc ggcatcatca tctgggggat
    15061 tggcaacggc gggcaaccga ccggtattca taacctgtgg agcaacggcg gcttcttcag
    15121 taacggctgg cttgggatgg tgatgtcgtt gcaaatggtg atgtttgctt acggtgggat
    15181 cgaaattatc gggattaccg ccggtgaagc gaaagatcct gagaaatcga taccgcgtgc
    15241 gattaactcc gtgccgatgc gtattctggt gttctacgtc ggtacgctgt tcgtcattat
    15301 gtctatctac ccgtggaatc aggttggcac tgccggtagc ccgttcgtgc tgacgttcca
    15361 gcatatgggc attacctttg ccgccagcat tcttaacttt gttgtgctga ctgcttcgct
    15421 gtcggcaatt aacagtgacg tatttggcgt aggccgtatg ctccacggta tggcagagca
    15481 gggcagcgca ccgaaaattt tcagcaaaac gtcgcgtcgc ggtattccgt gggttacggt
    15541 gctggtgatg actaccgcgc tattgtttgc ggtgtatctg aactacatca tgccggaaaa
    15601 cgtcttcctg gtgatcgctt cgctggcaac cttcgccacg gtatgggtgt ggattatgat
    15661 cctgctgtcg caaattgcct tccgtcgccg tttgccgcca gaagaagtta aggcgctgaa
    15721 atttaaagtg ccgggtgggg tagcaacgac catcggcggg ctgattttcc tgctctttat
    15781 tatcgggttg attggttatc acccggatac gcgtatctcg ctgtatgtcg gtttcgcgtg
    15841 gattgttgtg ctgttgattg gctggatgtt taaacgccgc cacgatcgtc agctggctga
    15901 aaaccagtaa tccttgcgtt ctgtaagccg gttcaggagg aaatcctgat ccggctattc
    15961 cgaaagttat ccgcccccgt cctcctcccc caaatatcct tcagatgatg agtgatcctg
    16021 cattaggcta tggcaaggtg atcagatttt catcacaggg gaattatgat gttaaatgca
    16081 tggcacctgc cggtgccccc atttgttaaa caaagcaaag atcaactgct cattacactg
    16141 tggctgacgg gcgaagaccc accgcagcgc attatgctgc gtacagaaca cgataacgaa
    16201 gaaatgtcag taccgatgca taagcagcgc agtcagccgc agcctggtgt caccgcatgg
    16261 cgggcggcga tcgatctctc cagcggacaa cctcggcggc gttatagttt caaattgcta
    16321 tggcacgatc gccagcgttg gtttacaccg cagggcttca gccgaatgcc gccagcacga
    16381 ctggagcagt ttgccatcga tgtaccggat atcggcccac aatgggctgc ggatcagatt
    16441 ttttatcaga tcctccctga tcgtttcgcc cgtagccttc ctcgtgaagc tgaacaggat
    16501 catgtctatt accatcacgc agccggacaa gagatcatat tgcgtgactg ggatgaaccg
    16561 gtcacggcgc aggcgggcgg atcaacgttc tatggcggcg atctggacgg gataagcgaa
    16621 aaactgccgt atctgaaaaa acttggcgtg acggcgttgt atctcaatcc ggtgtttaaa
    16681 gctcccagcg tacataaata cgataccgag gattatcgcc atgtcgatcc gcagtttggc
    16741 ggtgatgggg cgttgctgcg tttgcgacac aatacgcagc agctgggaat gcggctggtg
    16801 ctggacggcg tgtttaacca cagtggcgat tcccatgcct ggtttgacag gcacaatcgt
    16861 ggcacggggg gagcttgtca caaccccgaa tcgccctggc gcgactggta ctcgtttagt
    16921 gatgatggca cggcgctcga ctggcttggc tatgccagct tgccgaagct ggattatcag
    16981 tcggaaagtc tggtgaatga aatttatcgc ggggaagaca gtattgtccg ccactggctg
    17041 aaagcgccgt ggaatatgga cggctggcgg ctggatgtgg tgcatatgct gggggaagcg
    17101 ggtggggcgc gcaataattt gcagcacgtt gccgggatca ccgaagcggc gaaagaaacc
    17161 cagccagaag cgtatatagt tggagagcat tttggcgatg cacggcaatg gttacaggcc
    17221 gatgtggaag atgccgccat gaactatcgc ggcttcacat tcccgttgtg gggatttctt
    17281 gccaataccg acatctctta cgatccgcag caaattgacg ctcaaacctg tatggcctgg
    17341 atggataatt accgcgcagg gctttctcat caacagcaat tacgtatgtt taatcagctc
    17401 gacagccacg atactgcgcg atttaaaacg ctgctcggtc gggatgttgc gcgcctgccc
    17461 ctggcggtgg tctggctgtt cacctggcct ggtgtaccgt gcatttatta cggtgatgaa
    17521 gtgggactgg atggtaaaaa cgatccgttt tgccgtaaac cgtttccctg gcaggtggaa
    17581 aagcaggata cggcgttatt cgcgctgtac cagcgaatga ttgtgctgcg taagaaaagt
    17641 caggcgctgc gtcgtggcgg ctgtcaggtg ctgtatgcgg aagataacgt ggtggtattt
    17701 gtccgcgtgc tgaatcagca gcgtgtactg gtggcaatta accgtggcga agcctgtgaa
    17761 gtggtgctac ccgcgtcacc gtttcttaat gtcgtgcaat ggcaacgcaa agaaggccat
    17821 gggcaactga cagacgggat tctggctttg cctgccattt cggctacggt gtggatgaac
    17881 taaagcatct gttttatttg ttacgtcgtt agtcaccagc cctgacacta cgcagcgcaa
    17941 aaaagctgct caatgtcagg gctgaatttg ttaaatattt gtcccgatcg atatgagcga
    18001 tttttggtat tctttgccca tgatgttatg tacaacatgc tccaggataa ccctgtagtg
    18061 gagcaaagat at
//
LOCUS       DAZUCE010000094        17679 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZUCE010000094 DAZUCE010000000
VERSION     DAZUCE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.094"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            88..591
                     /locus_tag="ACHY03_002676"
     CDS             88..591
                     /locus_tag="ACHY03_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFS3905885.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLYFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRNELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(658..2115)
                     /gene="nuoN"
                     /locus_tag="ACHY03_002677"
     CDS             complement(658..2115)
                     /gene="nuoN"
                     /locus_tag="ACHY03_002677"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFS3905886.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2122..3651)
                     /gene="nuoM"
                     /locus_tag="ACHY03_002678"
     CDS             complement(2122..3651)
                     /gene="nuoM"
                     /locus_tag="ACHY03_002678"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFS3905887.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3815..5656)
                     /gene="nuoL"
                     /locus_tag="ACHY03_002679"
     CDS             complement(3815..5656)
                     /gene="nuoL"
                     /locus_tag="ACHY03_002679"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFS3905888.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5653..5955)
                     /gene="nuoK"
                     /locus_tag="ACHY03_002680"
     CDS             complement(5653..5955)
                     /gene="nuoK"
                     /locus_tag="ACHY03_002680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFS3905889.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5952..6506)
                     /gene="nuoJ"
                     /locus_tag="ACHY03_002681"
     CDS             complement(5952..6506)
                     /gene="nuoJ"
                     /locus_tag="ACHY03_002681"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFS3905890.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6518..7060)
                     /gene="nuoI"
                     /locus_tag="ACHY03_002682"
     CDS             complement(6518..7060)
                     /gene="nuoI"
                     /locus_tag="ACHY03_002682"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFS3905891.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7075..8052)
                     /gene="nuoH"
                     /locus_tag="ACHY03_002683"
     CDS             complement(7075..8052)
                     /gene="nuoH"
                     /locus_tag="ACHY03_002683"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFS3905892.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8049..10775)
                     /gene="nuoG"
                     /locus_tag="ACHY03_002684"
     CDS             complement(8049..10775)
                     /gene="nuoG"
                     /locus_tag="ACHY03_002684"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFS3905893.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10828..12165)
                     /gene="nuoF"
                     /locus_tag="ACHY03_002685"
     CDS             complement(10828..12165)
                     /gene="nuoF"
                     /locus_tag="ACHY03_002685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFS3905894.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12162..12662)
                     /gene="nuoE"
                     /locus_tag="ACHY03_002686"
     CDS             complement(12162..12662)
                     /gene="nuoE"
                     /locus_tag="ACHY03_002686"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFS3905895.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12665..14467)
                     /gene="nuoC"
                     /locus_tag="ACHY03_002687"
     CDS             complement(12665..14467)
                     /gene="nuoC"
                     /locus_tag="ACHY03_002687"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFS3905896.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14561..15223)
                     /gene="nuoB"
                     /locus_tag="ACHY03_002688"
     CDS             complement(14561..15223)
                     /gene="nuoB"
                     /locus_tag="ACHY03_002688"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFS3905897.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15239..15682)
                     /gene="nuoA"
                     /locus_tag="ACHY03_002689"
     CDS             complement(15239..15682)
                     /gene="nuoA"
                     /locus_tag="ACHY03_002689"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFS3905898.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16312..17250)
                     /gene="lrhA"
                     /locus_tag="ACHY03_002690"
     CDS             complement(16312..17250)
                     /gene="lrhA"
                     /locus_tag="ACHY03_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFS3905899.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4400 a         4944 c         4583 g         3752 t
ORIGIN      
        1 gcgggtccag aaacagcgac atgtacattt tattttccct cgtctgtttt aagatgcgaa
       61 acataaaatt caatggagga aaaataaatg ggaatgattg gttattttgc agaaattgac
      121 tcagaaaaga ttaaccaact gcttgagtct actgaaaaac ctttgatgga taatatacat
      181 gacacgcttt caggattacg ccgactggat attgataaac gctgggattt tttatacttt
      241 gggctgacag gcacctctgc gttcgatcca gcgaagaatg atccgctgag tcgtgccgtg
      301 cttggtgaac acagcctcga agatggcata gatggcttcc ttggattgac ctggaatcag
      361 gagttagccg cgacaatcga ccgactggaa tcactggatc gaaatgaact gcgtaaacag
      421 ttttccatta agcgactcaa tgaaatggag atttatccgg gagtgacctt ttcagaagag
      481 ttagaggggc aactctttgc ctcgatcatg cttgatatgg aaaagctgat tagcgcgtat
      541 cgacgtatgc tgcggcaagg gaatcatgcg ctgacggtta ttgttgggtg atggtgtcag
      601 ggcaccaaaa aaaaccgccg aacatgtcgg cggctttctg acttacaaac taacagatta
      661 catcagcggc attgctaacc gcacaatgct aatcagcggt tgtggccata cacccagcac
      721 cagtaccaac agtgcagaaa tcagcaccac gataccgccc gcgctgtact gccagtttga
      781 cggtgcatcg cgacccggtt gttccggtgc gtgcaggtac aggctcaccg ccacgcgcag
      841 gtagtagtag aggccgattg ccgaaccgac aaccacggca cccaccagcc accacaagtg
      901 tgcctggaca ccgaccgcca gcacgtagaa cttaccgata aagcccagcg tcatcgggat
      961 accggccaga gacagcatca tcaccgtcat caccgccgcg aggatcggac gatgccagaa
     1021 cagaccgcgg taagagaaca gggaatcagc atccgggcca cgatacgggc tggacatcag
     1081 gctgaccacg ccgaacgcgc caaggctgct gaacagataa ccggccaggt aaacccctac
     1141 cgcttccatc gacatctcgc cagtttgcag cgcaatcagc gccaccagca gatagccgag
     1201 gtgagagata gatgagtaac cgagcagacg tttgatattg gtctggctca gcgccatcag
     1261 gttaccgaag atgatggagg caaaggcgat aatcgccagc accacacgaa tcgcttcgct
     1321 gtcacccacc ggtgcgtaga ggaacagacg catcactaca ccgaagatag cgattttgct
     1381 cgccgtcgcc aggaaagtgg aaaccggcgc aggcgcgccc tggtatacgt ctggcgtcca
     1441 caggtggaac ggcaccagag agagtttgaa gccgaggcca acaatcatca ggccgaaacc
     1501 tgccagcaac agcggctcgt ttagcatacc gtcgccgagg tttttaccca gcgcgacaaa
     1561 cgacaggtcg ccagactgcg catacaccag cgccataccg aacagcagga aagaagacgc
     1621 tgcggcagaa aggatggtgt atttgatact ggcttccagc gaacgtttct ggcgaaaagc
     1681 gtaaccgacc aggccaaaca gcggcaaaga gatcagttca ataccgagga acagagacgc
     1741 cagatggttt gcattcgcca gcaggatccc gcccagcgcg gcaattaaca ccaacaggta
     1801 gaactcatcc ttgttgtcgt tatagccttc aagccacggg taggcgaaag tacaggtggc
     1861 gaggctcgcc aacaatacca gcccggtgta aagcatggcg aaaccatcaa cgcgcatcag
     1921 cggcgtaacg tccatagcgc ccgcctggcc aacaaaccag agcgaaacca gcgccgcgtt
     1981 aagcccaata accgagagcg tagcgttgag gaaatgattg cgtcgccacg caatggagag
     2041 catcacaacc accaccgtca agccgacgat cagcaacggt agcagtgcga tcaggttttg
     2101 tggagttatt gtcatggcga tttacggcct tgtagtagta acggaattaa caaaccactg
     2161 ctggatattg ccaatcgcgg agtgcgaggt atccagaatc ggctgcggat agaagcccag
     2221 cagtaccagc agcaccacca gcaacaggat cataaacagc tcacgcagcg acatccctgg
     2281 cagttcctgg ctggcaatct ggcttttcgc tttaccgaag taagcacgat gcaacatcgc
     2341 cagcgaataa acagatgcaa agaccagccc aaaggtagag ataacggtaa tcaccgggac
     2401 aacctggaag ctgccgaaca gaatcataaa ttcgccgacg aagttaccgg tgccaggcat
     2461 cccaagcgtt gccaccgcaa agaacagcga cagtgctggc agccatttca tcttgctcca
     2521 cagaccgccc atcatgcgca tgtcgcgggt atggatacgt tcataaagct gaccacaaag
     2581 aataaacaga cccgccgctg acaagccgtg cgcaatcatc tggattaccg cgccctggta
     2641 ggccaactgg ctgccggtgt agatagcaat cagtacgaag cccatgtggg aaaccgaggt
     2701 gtaggcgatc agacgtttga tatcggtctg ggcgaaggcc atccacgcac cgtagaagat
     2761 gccgataaca cccagccaca tagcgattgg cgcgaactct gccgacgcgt tcgggaacag
     2821 cggtagggag aaacgcagca gaccgtaagc ggcggttttc agcaagatcc ccgcgaggtc
     2881 cacggaaccg gcagtcggag cctgtgagtg cgcatccggc agccagccat gcagcggaac
     2941 caccggcatt ttcaccgcaa acgcgatgaa gaagcccagc atcaacagat attccacacc
     3001 actggacatt ggcgtattca acagctcttc atagttgaag gtccagacgc cggtcgcatt
     3061 gtagtgaaca aaaaccagcg ccaggatggc gatcaacatc accagaccac tcgcctgggt
     3121 gtatatgaag aacttggttg ccgccgtgat acgcgtttta ccgtcagagg ctttatgccc
     3181 ccacaatgcg atcaggaagt acatcggcac cagcatcatt tcccagaaga agaagaacag
     3241 gaacatgtcg atggcaagga acacgccgat aacgccgccc aggatccaca tcaggttgag
     3301 gtggaagaag ccctgatatt tttcgatctc tttccacgaa cagagtaccg ccagcacacc
     3361 gagcagaccg gtcagcacga ccatcagcag cgacagcccg tcaatggcga gatggataga
     3421 gataccaaaa cgcgggatcc acggcatgtc gaattcagac tgccactgcg gaattccggc
     3481 ggattgcgtc agtgaataac cgccctgcaa ccacagttgc agcgacagcg ccagcgtcaa
     3541 tcccatggtg atcagcgcga tccagcgcgg caccttgacg ccaaagcgtt cggtctgcca
     3601 gcacaggaag ccgccgataa agggaattaa tattagccag ggtagtaaca tggcgatctt
     3661 tattccttgt aaaagtcccg tcaggaccgg attttcaacg aattctcacg acagaaaatt
     3721 ctcttcggac tgggggtagt gtcggatgca ccgcgaagga ggcttatccg acctacgttc
     3781 ttatatgccc aggggcaatc ccacaatcct taactcaacg cagtaccatc aacagtgcca
     3841 gcaccacgac cgcaccgatg ctcatggatg ccacatacca gcgcagatag ccgttctcgc
     3901 ttaacagcag acctttacct gcaaagcggg aaagaaccgc cggaatgttc atcattgagt
     3961 tcagcggatc gcgtttcagc aaccaggcaa tacccaggaa cggcttgacg aacactttgt
     4021 cgtacagcca gtcaaagccc caggcgttgt accaccaggt gcccagcaga cggcccggcg
     4081 cactgttggc gatggaggtc accagagtac gtttacccag ccacagccag gctgccagca
     4141 gaatgccgac caccgcgacc acaccagagg taatttccag ggtcaacatg ctgccgtgcg
     4201 ccagttccgt cgtttgcgga agcacgccct gcagcggcgg tacaatcagt gcgccaacga
     4261 aggtggaaag gatcagcagc acaatcagcg gcaggctgtg agttacccct ttcacggcgt
     4321 gagcgtgaat ttgttctttt ccgtggaaga cgatgaaaat catacggaag gtgtagagcg
     4381 aggtcataaa cgcaccgacc agacctgcca ccatcagatt gatatgacca ttcgccatcg
     4441 cacccgcgag gatctcatcc ttactgaaga agcccgcagt gaccagcggc agtgccgaca
     4501 gtgctgcgcc gcccaccagg aagcagagat aaaccagcgg aatagattta cgcagaccgc
     4561 ccatcttgaa gatgttctgt tcgtgatggc aggccagaat gacagaaccg gatgccagga
     4621 acagcagcgc tttaaagaac gcgtgggtca tcaagtgaaa aatcgccgca tcccatgcct
     4681 gcacgccaag cgcgaggaac atgtagccaa tctggctcat ggtagagtaa gcgagaacac
     4741 gtttgatgtc ggtctgtacc agcgcggcaa aaccggccag cagcagcgta accgccccaa
     4801 caatgcccac cagatgcaga acttccggcg tcatcaggaa caggccgtgg gtacgggcga
     4861 tcaggtagac acccgcggtt accatcgttg cggcgtggat cagcgcggag acaggcgtcg
     4921 ggcccgccat cgcgtcggca agccatgtct gcaacggcaa ctgcgcagat ttaccgaccg
     4981 caccgcccag cagcatcagc gtcgcccaca tcagcatatt attgccgtca gcaaagtgcg
     5041 ctggtgccag ttccaccatt tcgcggaagt tcagggtgcc cagttcgttg taaagaatga
     5101 acagcgcgaa agcgaggaac acgtcaccca cacgggtcac gacgaacgct ttcattgccg
     5161 ctgcgccatt cttcggatcg gtgtaataga aaccgatcag cagataggag cataggccca
     5221 cgccttccca gccgaggtac atcagcagca ggttgtcggc aagcaccaga actaccatgc
     5281 tggcgatgaa caggttggtg taggcgaaga agcgagagta gccctcttca ccgcgcatgt
     5341 accaggaggc gtacatgtgg atcaggaaac ccacaccagt gaccaccgag agcatggtca
     5401 gcgacaggcc atccaacacc aggttaaaac cgatgttaaa gtcgcctacc gacatccacg
     5461 tccacagcgg ctggctgtaa gcctgctcgc cgttagcgaa gaagtcgacg ccaataaagg
     5521 cggttaccag cgccgccagg cccacagagc ctacgccgac gatcgccgag acgttttcag
     5581 accagcgccc acgggagaac gccagcagga cgaagccaat caatggcaaa ataatggtta
     5641 aggcaagcat gttcatccgc gcatctcact tactgaatcg atgttcaggt tctggcgacg
     5701 acggtgaagt tgcagcagca gcgcaaggcc gatactcgct tctgccgccg cgaggctgat
     5761 ggcgagaatg tacatcacct gaccgtcggt ctggccccag tagcttccgg cgaccacaaa
     5821 ggccagcgcg gaggcgttaa tcatgatttc cagaccaatc agcataaaca gcagattgcg
     5881 acggataacc agaccggtta agccaagaac gaataagatt gccgcgagga tcagtccatg
     5941 ttgtaagggg atcatgcgtg ctcctccgtt tttcttttcg cgctgtcgtc tttacgattg
     6001 ctcagcactt cacccgcacg ctcttcacga ccgacgtgga aggccacaac cagacccgcg
     6061 agcagcagca tagaagccag ttccaccgcc agtacgtaag gcccgaacag cgtaataccc
     6121 actgctttag cactgattgg cgtaccgtcg ataccttgat cgttaacacc gagaatggcg
     6181 taaacaatca ccaccaacat gatggctgac aaaattgccg gaccaatcca cacctgcggt
     6241 ttcagccact ggcgttcttg ttcgatttct gaaccgccca ggttgagcat catcaccacg
     6301 aacacgaaca gcaccataat ggcacccgcg tagacgataa tttccagcgc accggcgaag
     6361 taagcgccta gtgagaagaa caccccggaa atcgccagca gcgaaataat caggtacagc
     6421 agtgcgtgta ccggattggt atgggtgatc actcgcaagg tcgcaagtat ggctatcagg
     6481 ccacagatat aaaaagcgaa ctccattgcc cctctcctta cggtaacagg ctcttgacgt
     6541 cgataggctt ggcttcgttc tctgcttcgc ccttatcttt gccgtcgatt gccatacctg
     6601 ccatccggta gaagttatac tccgggtatt tgcccggacc ggagatcagc agatcctctt
     6661 tctcgtaaac cagatcctgg cgcttgtatt cccccatttc gaaatccggg gttaactgaa
     6721 tcgccgtggt cggacaggct tcttcgcaca aaccacagaa aatgcagcgt gagaagttga
     6781 tgcggaaaaa ttccggatac cagcgaccgt ctttggtttc tgctttttgc agcgagatac
     6841 agccgaccgg gcaggctacc gcgcagaggt tacaggctac gcaacgctct tcgccgtccg
     6901 ggtcgcgggt cagaacgata cgaccacgat aacggggcgg cagatagacc ggctcttccg
     6961 ggtacattcg cgtttcgcgt ttggcgaacg cgtgcaggcc gatcatccag atactacgaa
     7021 cctgggtgcc gaaacctact aacaattctt ttaaggtcat ggtcttattg ccccttattg
     7081 cgcctgccag agaatgacag ccgccgttac cagcaagttg atcagcgtca gcggcaggca
     7141 gattttccag ccgaaggaca ttacctggtc ataacgcgga cgcggtaacg acgcacgaat
     7201 caaaatgaac atcatcataa agaacgcggt tttcagcgcg aaccagatga atggtggtaa
     7261 caacgggcct tgccagccac cgaagaacag cgtcaccatc aatgcagaga tggtcacaat
     7321 cccgatgtac tcacccacga agaacagacc gaacttcata ccggaatatt caatgtggta
     7381 accatccgcc agttcctgct cggcttccgg ctggtcaaac gggtgacggt gacacaccgc
     7441 cacgcccgcg atggcaaagg taataaaacc aaagaattgc gggatgacgt tccacacatg
     7501 cgcctggctg ttaacgatgt cggtcatgtt gaatgaaccg gcttgcgcca ccacgcccat
     7561 cagggaaagc ccgaggaaca cttcgtagct cagggtctgc gcagaagcac gcatcgcacc
     7621 cagcagcgag tatttgttgt tacttgacca gcccgcaaac agcaccgcgt aaaccgccag
     7681 acctgccatc atcaggaaga acaaaatccc gatgttcagg tcggcaacca cccaacccgg
     7741 actgactggc acaattgcaa aggccagcag cagcgaggta aaggcaatca tcggtgctag
     7801 ggtaaagatg acgcgatccg agaatttcgg gatccagtct tctttaaaga acattttgat
     7861 catgtccgca accagctgga gcgaaccacc ccagccaaca cggttaggtc cgtaacggtt
     7921 ctggaacaga cccagcaggc gacgttcgcc aaagctcatg aatgccccgc aggtgacaac
     7981 caccagcagg atcaccaccg ctttgaggat ggtcagcagg atctcaatca gttccggtga
     8041 tatccaactc attgttgtgc ctccttgaga tcctcaagat gcgcgccagc cagcaccgga
     8101 gcaatgccgg acatacccat cggcaagccc acctgccctg ccgtcagccc ttcggcgatt
     8161 tcaaccggca gcgtgaccgt gttgccatcg taactaaagg agacgcgtgt acctgcgttc
     8221 acacccaact tcgcggcatc cgctgggttg agtttgatgt acggctgcgg catacgactc
     8281 tggaagaccg gagcacgctg tgacaattca tcgctgccaa acaggtggta gtacggcgcg
     8341 atacgccatt tcccgtcctg cggctggaag cgtgccggta cgctggtgaa gtaatccaga
     8401 ccattttcgc tggtttcaaa cagacgcacg cccggatcgc caaagcgcag tttgccgccc
     8461 acttcgtcct ggaatttgtt ccacgcctgc ggggagttcc agcctggcgc ccaggcaaac
     8521 ggcacttgcg aacggtgcgc agtcggctgg ttgttacctt ccatcgagaa ggtaaacatg
     8581 gtgtcaatat cctgcggctg acgtggctca tgaacgctaa tattggcgcg catggcggta
     8641 cgaccgctgt aacggtgcgg ttcacgggcc agtttctgcc cacgaatacg gaatgtcgca
     8701 tccggcgcag catctttgat acctgccagt tccgggattt tcgccacaac agcgtcaatc
     8761 acatggtcga gctgcgtcca gtccacttca cggctcaaca gggtactgtg cagcgagtgt
     8821 aaccagcgcc agctttccag catgacagtt ttgctgtcgt aataagccgg atcgtaaacc
     8881 tggaagaaac gttgggcgcg accttcatta ttaattaccg taccgtcgct ttcagcaaag
     8941 ctggcagcgg aaagtaccag atgggcgttt tccatgatcg ctgtgcgttg atggtcaacc
     9001 accatcacca gcggcgcttt agccagcgca gcattcacgc gggtagcaga agcgtgacga
     9061 tgcagatcgt tttccagcac caccaccgcg tcggcgcgtc cggtttccag ttcggttaac
     9121 gcttcttcaa gcgatccacc gcccataatg cccagcccca tgctgttgac ggaacgggca
     9181 atcatggtga taccgacgtc agcgccgcga cctttcaggg ctttcgcgac gttagccgca
     9241 gcctgaatta cttcagcgct accggcgttc gtcccggaga taatcaacgg tttcttcgca
     9301 cccgccagtg cctgcacgat gacgtcgatt ttgctttgca gttcaggttc aataccgtca
     9361 accgctggcg cggagttatc cagcgcatgg gcgatggcaa aacctaaacg cgcctgatct
     9421 tcaaccggtg cgcggtaagt ccacgccgcg atatcatcca gacgggtgtc atcaacgttg
     9481 gtaacaaaca gcggatgctt cgcacgttga ccgatgttga ggattgccgc aatctgccag
     9541 tcagccactt tctgtgctgc cgccatttcg cgcgctttac ctttcaccgc ctgacgcact
     9601 gccagcgcga cgcgcgcgcc ggtctgggta acgtcttcgc ccagcaccag taccgcatcg
     9661 taagattcga tttcgcgcag agcaggagta taaatgccgc cttcacgcag tactttcagc
     9721 gccagttgca gacgttcctg ctcaccgtga gcgataccgg tgtagaagtt ttcttcgccc
     9781 accagctcac gcagcgcaaa gttactttcc acgctggcgc gcggagaacc aatgccgatc
     9841 actttcttcg actgacgcag aatatctgct gcgccctgca ttgcctgttc ggcgttgagg
     9901 gtaatgaaat catcgccacg acgctgtact ggctgacgcg gacgatcttt caggttgacg
     9961 taaccgtaac cgaaacgacc acggtcgcag aggaagtagt ggtttaccgt accgttgtaa
    10021 cggttttcga tacgacgcag ttcgccataa cgctcgccgg ggctgatgtt acagccgatg
    10081 gaacattgct ggcagatgct cggcgcaaac tgcatatccc atttacggtt gtaacgctcg
    10141 gagtgcgttt tgtcggtaaa tacgccggtc gggcaaattt cgaccaggtt accggaaaat
    10201 tcgctttcca gcgtgccgtc ttccgggcga ccgaagtaga cgttgtcgtg cgcgccataa
    10261 acgcccagat ctgtaccgtc agcgtaatct ttgtagtaac gcacgcagcg gtagcaggcg
    10321 atgcagcggt tcatttcgtg agagatgaac ggccccaaat cctgattacg gtgggtacgt
    10381 ttggtgaaac ggtagcgacg gaagctgtgt ccggtcatca cggtcatatc ctgaagatgg
    10441 cagttaccgc cttcttcaca taccggacag tcgtgcgggt ggttggtcat taaccactcg
    10501 accacacttt cacggaactg tttcgcttct tcgtcgtcaa tggaaataaa ggtgccatcg
    10561 gaagccggtg tcatacagga catcaccagg cgaccacgcg tgtcttccgc gttttggtat
    10621 tgcttcaccg cacactggcg gcaagcaccg acgcttccca gcgccggatg ccagcaaaag
    10681 taaggaatat caaggcccag agacagacaa gcttccagca ggttgtccgc tccgttgacc
    10741 tcgtattctt tgccgtctac atgaattgta gccattagca tgcttccagt tttctcagtc
    10801 agagactgag cgttaatcga aattcggtta ccagcgctct ttcagcaggt tcggctgaat
    10861 cccattaatc aaatgggtat tgctgaacgg ctgtttgatc cccgcctcaa attcttcgcg
    10921 gaaatatttg atggcgctct gtaacggctc cacggcacca ggtgcgtggg cacagaaagt
    10981 tttacccggg cctaagaatc gacacagttg ctcaagtgtt tcgatatcgc ccggctgacc
    11041 ttcgccacgc tccagcgcac gcagaatttt cacgctccac ggcagaccgt cgcggcacgg
    11101 cgtacaccag ccgcaggact cacgggcgaa aaactcttcc aggttacgca ccagcgacac
    11161 catgttgatc tcatggtcaa ccgccatcgc cagcgccgta cccagacggc ttcccgcttt
    11221 accgatactt tcgaattcca tcggcagatc aaggtgcgct tcggtcagga agtcagtccc
    11281 cgcgccgcct ggctgccagg ctttaaattt cagaccatca cgcataccac cggcgtaatc
    11341 ttcgaggatc tcgcgtgcgg tggtgccgaa cggcagttcc cacagtcccg gatttttcac
    11401 ccgaccagag aagcccatca gcttggtgcc agcatcttta cttttcgaga tgttctgata
    11461 ccactccacg ccgttagcga ggatcgccgg aacgttacac agggtttcga cgttgttgac
    11521 acaggtcggt ttgccccata cgccggaggt tgccgggaag gggggtttcg agcgtgggtt
    11581 agcacgacgc ccttccaggg agttgattaa cgctgtttct tccccgcaga tgtagcgccc
    11641 tgccccggta tggacgaaca gttcgaagtc gaaacctgtt cccataatgt ttttgccaag
    11701 caagcccgct tcagtggctt cggcaatggc gcggcgcaga tttactgccg cttcgatata
    11761 ttcgccacgc aggaagatgt agccacggta agctttcagc gcaaacgcgg agatgagcat
    11821 accttccacc agcaggtgcg gcagttgctc catcaacagg cggtctttat aggtgcctgg
    11881 ctccatttca tcggcgttac acagcaggta acggatgttc atggattcgt ctttcggcat
    11941 caggctccat ttcaggccgg tagaaaagcc cgcgccaccg cgccctttca gaccagcgtc
    12001 ttttacctga ttaacgattt cgtccggaga cagcccggtc agcgccttac gcgccccttc
    12061 gtaaccgttt ttgctgcggt attcgtccag ccacactggc tgtttgtcat cgcgcagacg
    12121 ccaggtcagc ggatgcgttt cgggagtacg gataatgttt ttcatttata ccgctccagc
    12181 agttcaggga tcgcttccgg ggtcagatgc gcgtgagtgt cctcatcgat catcatgttt
    12241 ggccctttat cacagttccc caggcagcaa gttggcagca gcgtaaagcg gccatcaaat
    12301 gtcgtttgcc ctggtttgat gttcagcttt ttctcgagcg ccgcctgaat accctgatac
    12361 ccgttgatat gacagaccac gctgtcgcaa taacggatca catggcgacc aaccggctgg
    12421 cggaagatct gactgtagaa cgttgccaca ccttcgacgt cgcttgccgg aatacccagc
    12481 acatcggcga tcgcgtggat cgcaccatcc ggcacccagc cacgctgctt ctgaacgatt
    12541 ttcagcgctt caatggacgc cgcacgcggg tcttcgtagt ggtgcatctc gtgctcgatc
    12601 gcttcacgct ctgccgcact cagctcaaaa gcctcggttt gtggttgttg attctcgtgc
    12661 ataattagcg gtccacatct gacataacaa aatcgatact gcccagataa acaatcaggt
    12721 cagacaccag gctgccgcgg atcgccgccg gaatttgctg caaatgcgca taactcggcg
    12781 tgcggatacg agtgcgataa ctcatggtgc taccgtcgct ggtcaggtag taactgttga
    12841 tccctttggt cgcctcaatc atctggaagg attcattggc aggcatcacc ggcccccacg
    12901 acacttgcag gaagtgggtg atcagggttt cgatatgttg cagcgtgcgc tctttcggcg
    12961 gcggcgtggt cagcgggtga tccgctttga acgggccttc cggcatgttg ttgaggcact
    13021 gctcaagaat gcgcagactc tggcgcagct cttccacttt cagcattacg cgggtgtagc
    13081 agtcagaaac accaccacca accgggattt caaagtcgaa gttttcatag ccagaataag
    13141 gacgcgcctt acgcacgtcg aagtcgatcc cggtagcacg caggcccgcg ccagtggtgc
    13201 cccactccag cgcctctttc gcgccatagg cggcaacgcc ctgggaacga cctttcagaa
    13261 tggtattttg cagcgccgct ttctcgtaag acgccagacg tttcggcatc cagtcgagga
    13321 actcacgcag caggcgatcc cagccgcgcg gcaggtcgtg cgctacgccg ccaatacgga
    13381 accacgccgg gtgcatacgg aaacccgtga tcgcttccac cagatcgtaa attttctgac
    13441 gatcggtaaa ggcgaagaac acgggcgtca ttgcgccgac gtcctgaata aaggtcgaga
    13501 tgtacagcag gtggctgttg atacggaaca gttcggagag cataacgcga atgacgttaa
    13561 cgcgatccgg cacggtgatc ccggccagtt tctctaccgc cagcacgtaa ggcatttcgt
    13621 taacgcagcc gccgaggtat tcgatacggt cggtatacgg aatgtagctg tgccaggact
    13681 ggcgttcgcc cattttctcc gcaccacggt ggtggtagcc gatgtctggt acgcagtcga
    13741 caatctcttc gccatcgagc tgcaaaacga tacggaaagc accgtgcgcc gacgggtggt
    13801 tcggaccgag gttgaggaac atgaagtcct cgttttcggt gccgcgcttc atcccccact
    13861 cttccggttt gaaggtcagg gcttccatct ccagatcctg tttggctttg gtcagctcaa
    13921 acggcgagaa ttcggtagcg cgcgccgggt aatctttacg cagcgggtga cctttccagg
    13981 tttgcggcat catgatgcgg cgcaggttcg ggtgaccgtc gaatgtaatg ccaaacagat
    14041 cccaggtttc acgctcatac cagttagcgt tcgggaacag tttggtgaag gtcggtacgt
    14101 gcaggtcgtt ttctgccagc gccaccttca gcatgatgtc gcggttacga tcaatagaaa
    14161 tcagatggta gaaaacggaa aaatccgcgg caggtaaccc ttcgcggtgt gtgcgcagac
    14221 gttcgtccat gccgtgtaag tcaaacagca tgacgtaagg tttcggcagt ttctttaaga
    14281 aatcgccaac ttccagtaat tgttcacgct tgatccacac aacgggaacc ccggtgcgag
    14341 tcgcctgaac agtaaaggca tccggcccaa aacggttgcg cagttcgcca attaccggat
    14401 catcaagatg atcgcgggtc tgccaggcgg gttcttgcgc ggttaagtcg gtcatattgt
    14461 tcaccattgc aaatggtccg tggtgactgt cggcgcagta agcttcgcga aatagattta
    14521 ggaataagcg aagaaaaatc cctttgccga caggcgcaaa ttaaatctcg tcaggtgtac
    14581 gcaggttggt tacggcaatg cgttcaccgc gcttgcgttc gcgctctgat tgcatattgg
    14641 cgcgataaac gccctgatcg ccaaccaccc aggagagcgg acgacgttct ttgccgatag
    14701 attcctgcaa cagcatcagt gcctgcatgt acgcttcagg acgcggcggg cagcccggga
    14761 tatacacatc aaccgggatg aatttatcga cgccctgcac aacggaataa atatcgtaca
    14821 taccaccaga gttggcacag gcacccattg agataaccca ttttggttcc agcatctggt
    14881 catacagacg ctgaataacc ggtgccattt tggtaaagca ggttcctgca accaccatca
    14941 ggtcagcctg acgcggcgaa gcacgcaata cttctgcgcc aaaacgcgcc acgtcatgca
    15001 ccgcggtaaa cgaagtcacc atctcaacgt aacagcagga aagaccgaag ttatacggcc
    15061 aaattgagtt tttacgaccc cagttaacca tgtcattgag cttgcccata aacacgtttt
    15121 tgttaacttc ttgctccaga gggtcggtta cgatctcctg cttttgcagg gggtaacggt
    15181 cgttctcacc gttgggatct atgcgggtga gcgtataatc catcttaatg cctcgcggtt
    15241 agcgttgacg attagcgata ctgttcgttt ccgggttcat acgctcgcgg cgtgaacgcg
    15301 cgggcgtcca gtccagcgcg ccaatacgca ccagataaac cagacctgcc agtaacacaa
    15361 aaataaaaat tgcagcttcc acaaagccta cccagccgct ttcgcggata gaggttgacc
    15421 atgcgaacag atacagcgct tcaacgtcga agataacgaa gaacatggcc accagataaa
    15481 acttggcgga caggcgtaag cgggcggagc cgaccgagtc gataccggat tcaaacggca
    15541 cgtttttcga cctcgcgcgt gcgcgaccgc ctaaaaacca accgcctacc agcatcaggc
    15601 aacacaggcc aatggcaacg ataagaaaga tagcgaatgc ccagtgatga gcgatgactt
    15661 cagtggatgt tgacatactc attgcttact catcaaaagt agcgccagat tcactgctct
    15721 tcacggcaga tggacgccac atcgattcat ggggaggaat aaaaaaaacc ttacaatcac
    15781 tgtagaaatt cttttataca gctaattgat gtggtctttt actcctttct ataacctttt
    15841 gtcaacttta acaaaagttt cttcacatta gtttacataa tatcaacacc attagcattt
    15901 aatgcccttt caccccagat ccttgacgac tccaggataa ttagatgttg ttgaatcgtg
    15961 tccgttgtga agcaatggaa aaaatacggg tctattttga caggaatttg tgtcgattcc
    16021 tccccccaaa agagagtatt ttcttgatct gtgacacgct tttgtcattc cataacaaaa
    16081 acgcagcaac aaatttacgt attttttaac atcattgtag caggtgattt ttttcaggcg
    16141 attatttgtg cgtacggggc gtgaatctct ggtgattgaa aaatgaccag ttttgtacat
    16201 tctgcacgat gtgaagaagg ttctttacta aaaaaaagcc gctggggttt aaaacacccc
    16261 cagcggctcg tttttacact attgttccag gaattatcta tggtccgtcg attactcgat
    16321 atccctttca attaacaatg agtcatctcc ttccggagca gacattgggc tgtattgcca
    16381 cggattatgg tagctctcca tcgcctgata gatcacctgg gccagctcat tattactgga
    16441 cgggtcatag cacagcaaat attcggtatc aggtagcggc ggtaagccat ctactccact
    16501 cagcacacgc aagtccggac tcatcatctc gaccggcctt gccgtcacgc caagaccggc
    16561 tttcactgcc gcacgaacgg ccggaagcgt cgaggcgaca taagccagtc gccagggaat
    16621 atctgcttta ttaagcgtcg ccagcaccat atcgcgaaac ggacttggat catccagcaa
    16681 tacaagaggg atcggctcac ctttttgcaa tacgtattcc gctgcgcagt accagtgtgt
    16741 tggcgaggta cgcaggttca atgccttaaa cgctgaagga cgatgggtgg ttaccatcaa
    16801 atccacttcc tgggattcca gcatttctgc catataggca ttacgtttaa cgcggacatc
    16861 cagcgctaat ttcggataaa ccgaactcac gcgatttaac aggaaaggta agatcgtatc
    16921 ggcagattca tctgaagcac cgatagttaa cacgccctga agattactga acattaatga
    16981 tgagcaggcc tcatcattaa aacgcaggat tttcctggcg taaccaagaa gttgaatgcc
    17041 atgttcagtt aacagtttgt tgcgaccgtg acgagcgaac agttctttcc caacgagttg
    17101 ttccagacgc tgcatttgct gacttacggc ggactgagta cgacacacag cggcagctgc
    17161 ggcagcaaaa gtgttcagat cggcaacagc aacaaatgtt ctcagcagat cgaggtcgag
    17221 gttaattatc ggacgatttg cacttatcat atattatcac ttactggcgg ctcatactga
    17281 gctggttaat gctgtgcaca cacaaacaag caattccatt tgtaatgtgc ctccctggca
    17341 gtttcatccg gaacccggac gaaagtaaaa atgcatatga gttgcactaa aaaagcgact
    17401 cacattgttc cgttataatg cctgaagtag atcacagaat atatcttcag ggatcgcata
    17461 tctattaagt tactcactct tttctattta tgacatgcgc gtgtttgtat aaatgtaaat
    17521 gtgagtcctt gttccactct cgtgcagcat cgctggtcat acgcgaacac gtacaaacag
    17581 caatggtgag gcggcatcaa gagcagggat ccgtcattta tgcgagcatt ttaccccaaa
    17641 aaccttttat ttataagggt cattgcgaat tatctgatg
//
LOCUS       DAZUCE010000095        17636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZUCE010000095 DAZUCE010000000
VERSION     DAZUCE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.095"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            587..1915
                     /locus_tag="ACHY03_002691"
     CDS             587..1915
                     /locus_tag="ACHY03_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905900.1"
                     /translation="MRPISNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVTPLVPEEVHLLAVAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ESQNRVHEAARYSPAQQYGREETHFTNNGMREFGNISPLAWRNDVELQQSCNTSNIPP
                     NTANDDINNTRQTIEDEPQQYVKLTVDDMRKWAAMDQQARNALQGVPGWCTRNHFNIK
                     NARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKWCKERN
                     LAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGGAEKWS
                     KKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(2064..3572)
                     /gene="putP"
                     /locus_tag="ACHY03_002692"
     CDS             complement(2064..3572)
                     /gene="putP"
                     /locus_tag="ACHY03_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFS3905901.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFIAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQTLFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            3994..7956
                     /gene="putA"
                     /locus_tag="ACHY03_002693"
     CDS             3994..7956
                     /gene="putA"
                     /locus_tag="ACHY03_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS3905902.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDESPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPC
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPSGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QIMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSLVLWSDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7996..8634)
                     /gene="rutR"
                     /locus_tag="ACHY03_002694"
     CDS             complement(7996..8634)
                     /gene="rutR"
                     /locus_tag="ACHY03_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFS3905903.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8922..10013
                     /gene="rutA"
                     /locus_tag="ACHY03_002695"
     CDS             8922..10013
                     /gene="rutA"
                     /locus_tag="ACHY03_002695"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFS3905904.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKSIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMNDCRVSPQPRVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            10013..10705
                     /gene="rutB"
                     /locus_tag="ACHY03_002696"
     CDS             10013..10705
                     /gene="rutB"
                     /locus_tag="ACHY03_002696"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFS3905905.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNYHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10717..11103
                     /gene="rutC"
                     /locus_tag="ACHY03_002697"
     CDS             10717..11103
                     /gene="rutC"
                     /locus_tag="ACHY03_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFS3905906.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            11111..11911
                     /gene="rutD"
                     /locus_tag="ACHY03_002698"
     CDS             11111..11911
                     /gene="rutD"
                     /locus_tag="ACHY03_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFS3905907.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYVVVGHALGALVGMQLALDYP
                     ASVTVLVCVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHAVRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLTSLLHHREAAL"
     gene            11921..12511
                     /gene="rutE"
                     /locus_tag="ACHY03_002699"
     CDS             11921..12511
                     /gene="rutE"
                     /locus_tag="ACHY03_002699"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFS3905908.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12522..13016
                     /gene="rutF"
                     /locus_tag="ACHY03_002700"
     CDS             12522..13016
                     /gene="rutF"
                     /locus_tag="ACHY03_002700"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFS3905909.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            13037..14365
                     /gene="rutG"
                     /locus_tag="ACHY03_002701"
     CDS             13037..14365
                     /gene="rutG"
                     /locus_tag="ACHY03_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFS3905910.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14448..14621)
                     /gene="ymdF"
                     /locus_tag="ACHY03_002702"
     CDS             complement(14448..14621)
                     /gene="ymdF"
                     /locus_tag="ACHY03_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFS3905911.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            14994..15590
                     /gene="wrbA"
                     /locus_tag="ACHY03_002703"
     CDS             14994..15590
                     /gene="wrbA"
                     /locus_tag="ACHY03_002703"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFS3905912.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15611..15838
                     /gene="yccJ"
                     /locus_tag="ACHY03_002704"
     CDS             15611..15838
                     /gene="yccJ"
                     /locus_tag="ACHY03_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFS3905913.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15876..17117)
                     /gene="agp"
                     /locus_tag="ACHY03_002705"
     CDS             complement(15876..17117)
                     /gene="agp"
                     /locus_tag="ACHY03_002705"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFS3905914.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
     gene            complement(17400..>17636)
                     /locus_tag="ACHY03_002706"
     CDS             complement(17400..>17636)
                     /locus_tag="ACHY03_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905915.1"
                     /translation="LAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGN
                     HYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAI"
BASE COUNT         4263 a         4630 c         4591 g         4152 t
ORIGIN      
        1 aatcaatcac caaaaacacc aacacacaaa aatcaataag ttaataattg ttcagggctt
       61 ttttctcacg atttttctac tatgacatta tcacaaaccc actagcatga atacagaaag
      121 agaagtaata agatgagacg ccactgcttt cagatgaacc ctgtaattag ggtcgagaat
      181 atagtttatc cattccccta tcgataaatt aaaaaatttg cgtattaatt tttctctgac
      241 tgaaggctaa cgtcgaatta ccgacaagat tcacgagtga ttgtaactac gtactttatg
      301 aaacacagga agaaattatt atttaccgcg atgattcaag gcgtaaaaaa aataataaca
      361 ttcataaaac tcatcccgat ggcagcattg taaattcgcc aataactggc attcctcctg
      421 ctttttacgg gtgtttccat taacaccaga atgagcggat tgtttactac atcaagaacg
      481 catccgaagt gtacatcctg gtgcgccgac tgaatcgccc ggagccatgc ggatggctac
      541 gacttaatac gaatcattaa ccctgtaagg atacggagag catgacatga gaccaatatc
      601 caacctcaat acgagcgcag caacctatat tccaccgcaa caactcccga gtagttgtct
      661 ggaaactttg acgcttctgg tgcagatgaa taattatatc aaaagggatg cggactacag
      721 cacgggcatg gctgttactc cactggttcc tgaagaggtt cacttgctgg ctgttgcgat
      781 gacaaccgaa ctacatcgac atcagttcca accggtggta agtgcaaatg atctgcaact
      841 tcctgaaccg ttcaccttcg atgttgccgg tttcaccatc accttcacga agacgcaaga
      901 gcatgacaac acgggcaatc tcatgaaaat tgcagtcagc aagagcggtg tgtgcaccag
      961 tacaaatatt actcttgagc tgtttcactc cattgtcaca accctgatgt cacgcagtca
     1021 atatggaacc tttgatctct ggtccgtacg gccaatactc acggatgaaa gtcaaaacag
     1081 ggtgcatgaa gcagctcgct actccccggc ccagcaatac ggaagagaag aaacacactt
     1141 taccaacaat ggcatgcgag aatttggaaa tatttctccg ctggcgtggc gtaacgatgt
     1201 ggaattacag caatcgtgta atacaagcaa catcccacca aatactgcaa atgacgacat
     1261 aaacaacacc aggcagacca tcgaggacga gccacaacaa tatgttaaat tgactgtaga
     1321 cgacatgcga aaatgggcgg ctatggacca gcaggcgcga aacgctctac agggagttcc
     1381 aggatggtgt acgcgcaatc attttaatat caaaaatgcc agaaattacc tgaccgatca
     1441 cggcctgaac tacgcgggac aggtaaaggt aaacaggccg catgaatatg ccaaattcac
     1501 ccttgaacat atccgccagt gggcagcctt aaacaagcat gtgcgaaaac cggttggtta
     1561 tttagaaaag tggtgtaagg aaagaaacct cgcgccaact actgccagga attacctgaa
     1621 gaacgacggg cttaccgccc tgggtgagtt gaagctgaca gggccgcaga aatgggtcac
     1681 gttcaccttc ggagacatcg tacaatgggc gaatatgacc caggaggagc gtaacagcgc
     1741 agggggagcc gaaaagtgga gtaaaaaacg cggcttccag tggtctactg ccagatccta
     1801 cctgaaatcg agcggagtga ccaagcaagg tgcgagaaaa ctggcgtggt taaaaaacag
     1861 cgggaatatg tcgaatccat tctatctcgc ccaaccaacc agccgcagga cataagctga
     1921 aggtaccgat agcggcaatt ttgttcatct gtgccgggtg ggcaaatagc aattgccccc
     1981 gaacactccc tgccaggcaa cttataccgc atggcaggga acattcgggt taaaaagcaa
     2041 aagataaata attactcttc agcttagcct tcctgcaacc gtgacggcgg agccgaatga
     2101 tagtgcgcat cggcctcggc aaagcgtttt tgcatcgccg ctgacggcgc tttacccagc
     2161 aaactaaaca ctacaatccc aatactgccg aagataaagc ccggaataat ttcgtacagt
     2221 cccagccagc cgaactgttt ccagacgata accgtcagcg ccccgatgat catccccgcc
     2281 agcgcaccgt tgcgcgtcat gcgtgaccac atcaccgaga acagcaccac cggaccaaac
     2341 gccgcgccaa agcctgccca cgcgtagctc actaagccca gcacgcggtt ttccgggttt
     2401 gccgccagcg caatggccac cagcgccacc accagcacca tcacacgccc tacccacacc
     2461 agctctttct ggctggcatg tttacgcaga aacgctttat atagatcttc ggtaatcgca
     2521 ctggaacata ccagcagctg gcaacttaag gttgacatta ccgccgccag aattgccgac
     2581 agcagaatcc cggcaatcca cgggttaaac agagtttgcg ccagttcgat aaacacgcgt
     2641 tcagcgttct ggtttaccgc acctgctacc gccgggtgct cgttaaagta agcgatacca
     2701 aagaagccga cagccaccgc ccctgccagg cagaggatca tccaggtcat actgatacga
     2761 cgcgcatgga cgatgctgtg gtgagaatcc gccgccataa aacgcgccag gatgtgaggc
     2821 tgcccgaagt aacccagccc ccagcccatc agtgagataa tggcgataaa attcagccct
     2881 ttgagcatat ccacgttttc aatgctcttt tgtttgatca cttccagcga atcaccaaac
     2941 ccgccgacac tgataatgac gataaccggc gtcaggatca gggcaaaaat catcaggctg
     3001 gcctgtacag tgtcagtcca gctaaccgcc aggaaaccgc caataaaggt gtaaaggatc
     3061 gtcgccgcag ctcccgccca cagagccgtt tcgtagctca tgccaaaggt actttcaaac
     3121 agacgcgcgc ctgccacaat gcccgaagcg caataaatgg tgaagaacag caaaataacc
     3181 agcgcggaga taatgcgcaa aatgcggctt ttatcttcaa agcgcccggt gaaataatcc
     3241 ggcagagtca aggcgttatt gttgtattcg gtatgaacac gcaaccgtcc ggccaccagc
     3301 ttccagttaa tccacgcgcc taatgtcagg ccaatggcga tccagctttc ggaaatcccg
     3361 gaaagaaaaa ccgcgcccgg caaccccatt aataaccaac cgctcatgtc cgacgcgcct
     3421 gccgataatg cagtcacgaa tggcccaagg ctgcggccgc ccagaatata gtcgtcaaag
     3481 tttttggttg atcgccaggc gataaaccca atcaatatca tgccaaagat atagacacaa
     3541 aatgtcacca acatcggtgt gctaatagcc atctaaagtc tccaaaaatt attatcggca
     3601 atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt atgtattacc
     3661 agggcgaccg tatcctgccg gaagtgctgg ttattcacaa tcgatttaac acatcattta
     3721 catcaaattt tgttgcccag cgatactatt tttcatcagg ttgcacttcc tcacattttt
     3781 cgcggttgca cctttcaaaa atgttaactg ccgcagagaa aaagtctgca ttactttttt
     3841 aatccccgtc atatcgaatt cttttattaa catttcattc atttttaagc ttgctacgta
     3901 agtcacattt aacgtagttg cacaaagtcg caacataata gatatttcac gataacgtta
     3961 agttgcactt ttcagaacaa caggagtaat ggcatgggaa ccaccaccat gggggttaag
     4021 ctggacgacg cgacgcgtga gcgtattaag tctgccgcga cacgtatcga tcgcacaccg
     4081 cactggttaa ttaagcaggc gattttttct tatcttgaac aactggaaaa cagcgatacc
     4141 ttgccggagc tgcctgcgct gctttctggc gcggccaatg agagtgatga atcgccgact
     4201 ccggcagagg aaccacatca gcctttcctc gactttgccg agcaaatttt gccccagtcg
     4261 gtttcccgtg ccgccatcac cgccgcctat cgccgcccgg aaaccgaagc ggtttctatg
     4321 ctgctggaac aagcccgcct gccgcagcca gttgctgaac aggcacacaa actggcgtat
     4381 caactggccg ataaactgcg taatcaaaaa aatgccagtg gccgcgcagg tatggtccag
     4441 gggttattgc aggagttttc gctgtcatcg caggaaggcg tggcgctgat gtgtctggca
     4501 gaagcgttgt tgcgtattcc cgacaaagcc acccgtgatg cgttaattcg cgacaaaatc
     4561 agcaacggta actggcagtc acacattggt cgtagcccgt ccctgtttgt taatgccgct
     4621 acctgggggc tgttgtttac tggcaaactg gtttccaccc ataacgaagc cagcctctcc
     4681 cgctcgctga accgcattat cggtaagagc ggtgaaccgt tgatccgcaa aggtgtggat
     4741 atggcgatgc gcctgatggg agagcagttc gtcacgggcg aaaccattgc agaagcgtta
     4801 gccaatgccc gcaagctgga agagaaaggt ttccgttatt cttacgatat gcttggcgaa
     4861 gctgcactaa ctgccgcaga tgcacaagcg tatatggttt cctatcagca ggcgattcac
     4921 gccattggta aagcgtctaa cggtcgtggc atctatgaag gtccgggcat ttcaatcaag
     4981 ctgtcggcgc tgcatccgcg ttacagccgc gcccagtatg accgggtaat ggaagagctt
     5041 tacccacgtc tgaaatcact caccctgctg gcgcgtcagt acgatattgg tatcaacatt
     5101 gacgccgaag aggccgatcg tctggagatc tccctcgatc tgctggaaaa actctgtttc
     5161 gagcctgagc tggcaggctg gaacggcatc ggctttgtta ttcaggctta tcaaaaacgc
     5221 tgtccgttgg tgattgatta cctgattgat ctcgccaccc gcagccgtcg ccgtctgatg
     5281 attcgcctgg tgaaaggcgc gtactgggat agtgaaatca agcgtgcaca gatggacggc
     5341 cttgaaggtt atccggttta tacccgtaag gtgtataccg acgtttctta tctcgcctgt
     5401 gcgaaaaagc tgctggcggt gccgaatcta atctacccgc agttcgcgac gcacaacgcc
     5461 catacgctgg cagcgattta tcaactggca ggtcaaaact actatccggg tcagtacgag
     5521 ttccagtgcc tgcatggtat gggcgagcca ctgtatgagc aggtcaccgg aaaagttgcc
     5581 gacggcaaac ttaaccgtcc gtgtcgtatt tatgccccgg ttggcacaca tgaaacgctg
     5641 ctggcgtatc tggtgcgtcg cctgctggaa aacggtgcta acacctcgtt tgttaaccgt
     5701 attgccgaca cctctttgcc actggatgaa ctggtcgccg atccggtcac tgctgtagaa
     5761 aaactggcgc aacaggaagg gcaaactgga ttaccgcatc cgaaaattcc cctgccgcgc
     5821 gatctttacg gtcacgggcg cgacaactcg gcagggctgg atctcgctaa cgaacaccgt
     5881 ctggcctcgc tctcctctgc cctgctcaat agtgcactgc aaaaatggca ggccttgcca
     5941 atgctggaac aaccggtagc ggctggtgaa atgtcacccg ttattaaccc ggcagagccg
     6001 aaagatattg tgggctatgt gcgtgaagcc acgccgtgtg aagtagaaca ggcgctggaa
     6061 agtgcggtta ataatgcgcc aatctggttt gccacgcctc cggctgaacg cgcggcgatt
     6121 ttgcatcgcg ctgccgtgct gatggaaagc cagatgcagc aactgattgg tattctggtg
     6181 cgtgaagccg gaaaaacctt cagtaacgcc attgccgaag tgcgcgaagc ggtcgatttt
     6241 ctccactact acgccggaca ggtgcgggat gatttcgcta acgaaaccca tcgtccatta
     6301 gggcctgtgg tatgtatcag cccgtggaac ttcccgctgg cgattttcac cgggcagatc
     6361 gctgccgcac tggcggcagg taacagcgta ctggcaaaac cggcagaaca aacgccactg
     6421 attgctgcgc aagggatcgc cattttgctg gaagcgggcg taccgtcagg cgtggtgcaa
     6481 ttgctgccag gtcaggggga aaccgtgggc gcgcaactga cgggtgatga tcgcgtgcgc
     6541 ggggttatgt ttaccggttc aaccgaagtc gctacgttgc tgcaacgcaa tatcgccagc
     6601 cgcctggacg ctcagggtcg ccctattccg ctcatcgctg aaactggcgg tatgaacgcg
     6661 atgattgtcg attcctccgc cctgaccgaa caagtcgtaa tagatgtgct ggcctcggcg
     6721 ttcgacagtg cgggtcagcg ttgttcggcg ctgcgcgtgc tgtgcctgca agatgagatt
     6781 gccgaccaca cgttgaaaat gctgcgcggc gcaatggccg aatgccggat gggtaatccg
     6841 ggtcgcctga ccaccgatat cggtccggtg attgatagcg aagcgaaagc caatatcgag
     6901 cgtcatattc agatcatgcg tagtaaaggc cgtccagtgt tccaggcggt gcgggaaaac
     6961 agcgaagatg cccgtgaatg gcaaagcggc acctttgtcg ccccgacgct gatcgaactg
     7021 gatgactttg ccgaattgca aaaagaggtc tttggtccgg tgctgcatgt ggtgcgttac
     7081 aaccgtaacc agctgccgga gctgatcgaa cagattaacg cttccggcta tggtctgacg
     7141 cttggtgttc atacacgtat tgatgaaacc atcgcccagg tcactggctc agcccatgtt
     7201 ggtaacctgt atgttaaccg taatatggtg ggcgcagtgg tgggtgtgca gccattcggc
     7261 ggcgaaggat tgtccggcac cgggccaaaa gcaggcggtc cgctctatct ctaccgtcta
     7321 ctggcgaatc gcccggaaag tgcgctggca gtgacgctcg cgcgtcagga tgcagagtat
     7381 ccggtcgatg cgcagttgaa agccgcattg actcagccgc taaatgcact gcgggaatgg
     7441 gcagcaaatc gtccagaatt gcaggcgtta tgtacgcaat atggcgagct ggcgcaggca
     7501 ggaacacaac gattgctgcc agggccgacg ggtgaacgca acacctggac gctgctgccg
     7561 cgtgagcgcg tattgtgtat cgcggatgat gagcaggatg cgctgactca gctcgccgcc
     7621 gtgctggcgg tgggcagcct ggtgctgtgg tcggatgacg cgctgcatcg tcagttagtg
     7681 aaggcattgc catcggcagt cagcgaacgt attcaactgg cgaaagcgga aaatataacc
     7741 gctcaaccgt ttgatgcggt gatcttccac ggtgattcgg atcagcttcg cgcattgtgt
     7801 gaagccgttg ccgcgcggga tggcgcaatt gtttcggtgc agggttttgc ccgtggcgaa
     7861 agcaatatcc ttctggaacg gctgtatatc gagcgttcgc tgagtgtgaa taccgctgcc
     7921 gctggcggta acgccagttt aatgacgatc ggttaaacag gtagccggag gatgttacac
     7981 cttcctccgg catcattagc gtggtcgaat cccctcaata ataatccgct gcacgttttc
     8041 aaccgtttga ttgaaaaata cctcatcgcg caacgttgcg cctgtcactg cctccacctg
     8101 aggggcgaaa tcggcgtaat gttgagtgga agcccaaatc ataaaaatca aatgctgcgg
     8161 atcaattggc gcgagtttgc cgcttttgac ccaaccggca atcagcgccg atttctcatc
     8221 aattaatgct ttcaaatcgc ccgtcagttc atccattaac aacggcgcac ctgccagcat
     8281 ctccatacag aacaggcgcg aagcctgcgg ataatcgcgg gagacttcca gcttcagacg
     8341 gatgtactct ttgatcgccg ccagcggggc gaaatcttca cgaaacgctt ttaacggtgc
     8401 cagccagata tcgagaatct gccgcagcac ggcaatatac agcgcctctt ttgacggaaa
     8461 gtaatacagc agattggttt ttgaaacacc cgccaactct gcgatctgct ccagcctagt
     8521 gccgtgaaaa ccgaattgtg aaaaagtgtc cagtgctgcg ctaagaatcg cttttttctt
     8581 cgcgcttact gcacgcgaac gtttacccgt tgttttgact gcgccttgcg tcatgcgctc
     8641 tcccctcttg tagattggca acaggatagc aaaaccccca cccggcctcg accttctgca
     8701 ctctcatcgc gcacgaatgc atgttttatg tgcaactgtt ttgaccgttt agtccacttt
     8761 tttccagata ttttagataa cttaacatta acacattaat aacattcact tttaaaaaac
     8821 tggcatccac tttgcaaaca agccattacg cggctgaaaa ggatatgcag gatgcagtgc
     8881 cccgtctgac tttcacttta cgagacgaag agaggttgat gatgaaaatt ggcgtattcg
     8941 tacctattgg caacaacggc tggctcattt cgacccacgc gccgcagtac atgccgacct
     9001 ttgaactgaa taaatccatt gtgcaaaaag cggagcacta ccatttcgat ttcgccctgt
     9061 cgatgatcaa actgcgtggc tttggcggca aaactgagtt ctgggatcac aaccttgagt
     9121 cgttcacctt gatggcgggg ctggcagccg tgacctcgcg cattcagatt tacgccactg
     9181 ccgccacctt aacgttacct ccggcaatcg tcgcccgtat ggcggcaacc atcgactcca
     9241 tctctggcgg gcgttttggc gtcaacctcg tgactggctg gcaaaagccc gagtatgagc
     9301 agatgggtat ctggcctggc gatgactatt tctcccgtcg ttacgactat ctcaccgaat
     9361 atgttcaggt gctgcgcgac ctgtggggct cggggaaaag cgattttaaa ggcgattttt
     9421 tcaccatgaa tgattgtcgc gtcagtccgc aaccgagagt acccatgaaa gtgatctgcg
     9481 ccgggcaaag cgacgctggc atggcgttct ccgcccagta tgccgatttc aacttctgtt
     9541 tcggcaaagg cgtaaatacc cccacggctt tcgccccgac cgctgcgcgg atgaaacagg
     9601 ccgcagagca aaccgggcgc gacgttggct cttatgtatt gtttatggtg attgccgatg
     9661 aaaccgacga tgccgctcgc gccaaatggg aacactacaa agcgggcgcg gatgaagagg
     9721 cgttaagctg gctaaccgaa caaagtcaga aagatacccg ctccggtact gacaccaacg
     9781 tccgtcagat ggccgatccc acttcggcgg taaacatcaa tatggggacg ttagtcggtt
     9841 cttacgccag tgtcgcgcgc atgttagatg aagtcgcaag cgtgcctggt gccgaaggcg
     9901 tgctgttaac cttcgacgat tttctgtcgg gaatcgaaac cttcggcgag cgcattcaac
     9961 cactgatgca gtgccgcgcc catctccctg tgctgactca ggaggtggca tgatgacgac
    10021 attaaccgct cgaccggaag ccattacctt cgatccgcag caaagtgcgc tgatcgtggt
    10081 ggatatgcaa aacgcctatg ccacgccagg cggctactta gatctcgccg ggtttgatgt
    10141 ctcaaccact cgcccggtca ttgccaacat tcaaaccgcc gtgaccgcag cgcgagcggc
    10201 agggatgttg atcatctggt ttcaaaatgg ctgggatgaa cagtatgtcg aggctggcgg
    10261 ccctggctca ccgaattatc ataaatcgaa cgccctgaaa accatgcgta agcagccgca
    10321 gctgcagggg aaattgctgg cgaaaggctc ctgggattat caactggtgg atgaactggt
    10381 gccgcagcct ggcgatattg tgctgccgaa gccgcgctac agcggtttct tcaatacgcc
    10441 gctggacagc attttgcgca gccgcggaat acgccatctg gttttcaccg gcatcgctac
    10501 caacgtctgc gtcgaatcga cgctacgcga tggctttttt ctggagtatt tcggcgtggt
    10561 gctggaagac gcaacacacc aggcggggcc ggaatttgca cagaaagccg cgttgttcaa
    10621 tatcgaaacc ttttttggct gggtcagcga cgtcgaaacg ttctgcgacg cgctttctcc
    10681 cacgtccttt gctcgtatcg cttaaggagt ttaacgatgc caaaatccgt aattattccc
    10741 gctggcagca gcgcaccgct ggcccccttc gttcccggca cgctggctga tggcgtggtg
    10801 tatgtctccg gtacgctggc ttttgatcaa cacaataacg tgctgtttgc cgatgaccca
    10861 aaggcgcaaa cccgccacgt tctggaaact atccgcaagg tgatcgagac agcgggtggc
    10921 acgatggcgg atgtgacctt caacagcatc tttattaccg actggaaaaa ttacgccgcg
    10981 attaacgaaa tctacgccga gttttttcca ggagataaac cggcgcgatt ctgcattcag
    11041 tgcggactgg taaaacctga cgcgctggtg gaaatcgcca caattgcgca tatcgccaag
    11101 tgaggccgcg atgaaacttt cactctcacc tcccccttat gctgatgcgc ccgtagtggt
    11161 gttgatttcg ggtcttggcg gcagcggcag ttactggtta ccgcaactgg cggtgctgga
    11221 gcaggagtat caggtagtct gttacgacca gcgcggcacc ggcaataatc ccgacacgct
    11281 ggcagaagat tacagtatca cccagatggc agcggaactg catcaggcgc tggtagccgc
    11341 agggattgag cattacgtag tggtcggcca tgcgctcggt gcgctggtgg gaatgcagct
    11401 ggcgctggat tatcccgcgt cggtaactgt gctggtctgc gttaacggct ggctacgaat
    11461 aaacgcccat acgcgccgct gttttcaggt tcgcgaacga ttactgtata gcggtggcgc
    11521 gcaggcatgg gtggaagcgc agccgttgtt cctctatccc gccgactgga tggcggcccg
    11581 cgcacctcgc cttgaggcgg aagacgcact ggcactggcg cattttcagg gcaaaaataa
    11641 tttactgcgt cgacttaacg ccctcaaacg agctgacttt agtcatcatg cggttcgcat
    11701 ccgctgcccg gtgcaaatca tctgcgccag tgatgatctg ctggtgccat cagcatgttc
    11761 cagtgaactt catgccgccc tgcccgatag ccagaaaatg gtgatgcgct atggcggaca
    11821 cgcctgcaac gtgaccgatc ccgaaacgtt taatgctctg ttactcaacg ggcttaccag
    11881 cctgttacat caccgtgaag ccgccctgta aggaattgct atgaacgaag ccgttagccc
    11941 aggtgcgctt agcaccctgt tcaccgatgc ccgcactcac aacggctggc gggagacccc
    12001 cgtcagcgat gagacgttac gggagcttta tgccctgatg aaatgggggc cgacatcagc
    12061 taactgttct ccggcacgta tcgtgtttat ccgcactgca gaaggaaaag aacgtctgcg
    12121 cccggcgctt tccagcggca atctgcaaaa aaccctgacc gcgcccgtca ccgctatcgt
    12181 cgcatgggac agtgaatttt atgaacggtt accgcaactg tttcctcacg gcgatgcccg
    12241 cagttggttt acctccagcc cacaacttgc cgaagaaaca gcgtttcgca acagttccat
    12301 gcaggcggct tatcttatca tcgcctgccg tgcgctgggg ctggataccg gcccgatgtc
    12361 gggttttgat cgtcaacacg tagacgacgc tttttttgca ggcagcacgc tgaagagcaa
    12421 tctgctgatt aatatcggct atggcgatag cagcaaactt tttgcgcgcc tgccacgtct
    12481 gtcctttgaa gaagcctgcg ggctgttata aggagcaaac tatgaacatt gtcgatcaac
    12541 aaacttttcg cgatgcgatg tcctgcatgg gcgcggcggt caatatcatc accacggacg
    12601 gtccagccgg gcgagccggg ttcaccgcca gcgccgtctg cagcgtgacc gacacgcctc
    12661 ccacattact ggtgtgcctg aatcgtgggg cgtccgtctg gccggtattc aatgaaaatc
    12721 gaacgctgtg tgtaaataca cttagcgccg ggcaggagcc gctttcaaac ctttttggcg
    12781 gcaaaacgcc catggaacac cgctttgccg ccgcccgctg gcagactggt gtgaccggat
    12841 gcccgcaact ggaagaggcg ctggtttcgt ttgactgccg tatcagccag gtggtgagcg
    12901 tcggcaccca cgacattttg ttttgcgcca tcgaagcgat tcatcgtcac gccacaccct
    12961 acgggctggt gtggttcgat cgcagttatc acgcgctgat gcgccctgct tgttaacctc
    13021 cttaaggaga cagctgatgg caatgttcgg tcttcctcac tggcagttaa aatcgacctc
    13081 tacagagagc ggcgtggtcg cgccggatga acgattgccg tttgctcaga cggcaataat
    13141 gggcgttcaa cacgcggtgg cgatgtttgg cgcgacggtg ttaatgccga ttctgatggg
    13201 gctggatccc aatctttcca ttttaatgtc agggatcggc acgttactgt ttttctttat
    13261 taccggcggg cgcgtgccca gttatctcgg ctccagcgcg gcttttgtcg gcgtggtgat
    13321 cgcggcaacg ggctttaacg gtcagggcat taacccgaat atcagcattg cgctgggcgg
    13381 gattatcgcc tgtgggctgg tttataccgt cattggcctg gtggtgatga aaatcggtac
    13441 gcgctggatt gaacggctaa tgccgccggt ggtgacgggt gcggtggtga tggcgattgg
    13501 cctgaactta gcgcctatcg cggtaaaaag tgtatcggct tcaggctttg atagctggat
    13561 ggcggtaatg acggtgctgt gtattgggct ggtggcggtg tttacccgtg gcatgatcca
    13621 gcggttattg attcttgtag gattaattgt cgcctgtctg ctgtatggcg tgatgaccaa
    13681 cgttttaggg ctgggaaaag ccgtcgattt tactctcgtc agccatgctg cctggttcgg
    13741 tctgccgcat ttttccactc ctgcttttaa tggtcaggca atgatgctga ttgcacccgt
    13801 ggcggtgatt ctggtggcgg aaaacttagg ccatctcaag gcggtcgccg ggatgaccgg
    13861 acgcaatatg gatccgtata tgggacgggc attcgtcggt gatgggctgg cgacgatgct
    13921 ttctggttct gtcggtggca gcggcgtaac cacctacgcc gagaatatcg gtgtaatggc
    13981 cgtgacaaaa gtctactcga cgctggtgtt tgtcgctgct gccgtcatcg ccatgctatt
    14041 gggcttttca ccgaaatttg gcgcgctgat tcataccatt ccggccgcgg tgattggtgg
    14101 tgcatcaatt gttgtgttcg ggctgattgc cgtcgcaggc gcaaggatat gggtacaaaa
    14161 ccgtgtcgat ttaagccaga acggtaattt gattatggtc gcagtaacct tagtgctggg
    14221 cgcgggtgat tttgctctca cgctgggcgg ttttacgttg ggagggatcg gtacagcaac
    14281 ctttggcgcg attttactca atgcgttatt aagcagaagg ttggttgacg ttccaccgcc
    14341 agaagttgtt catcaggagc cataagcaga aaaccgccgg gattaccggc ggtgatgact
    14401 ttttgacgag cttaagaaaa aaatgcattc gtcattgatt agcccggcta gttgtcgctt
    14461 ttgccgtgac tgctcttgcc acctttttta cctgcttcag atgcgcgctg cggatcattc
    14521 ttgaaattac ccccgctgtg ctgtccacct tttttacctg cttctgatgc tctttcgcgg
    14581 tcttctgcaa aattgccgga accgcctcga tggtttgcca ttactgacct ccgtctgggt
    14641 atagacatca tattgcatag aattaacgcg gcgtatggcg gctgccatat tttcgctacg
    14701 tgggactaag cttagtgaac tgacgtgttt ctacccgcgt ttggtaatat cctgcaacag
    14761 cttcttagcg agaatagata tcatctccaa taattatcca taagccgctc ttatgattaa
    14821 aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa attgcgtgct tgtgaaagtt
    14881 gttataaatc aaataagtgg ttgtgaaatt tgcactctga aaaggacgtc ttatctttaa
    14941 ataagtggta gcgaatcgct acggaataga gataacacga ggagtggtta gaaatggcta
    15001 aagttctggt gctttattat tccatgtacg gacatattga aacgatggca cgcgcagtcg
    15061 ctgagggtgc aagcaaagtg gatggcgcag aagttgtcgt taagcgtgta ccggaaacca
    15121 tgccgccgca attatttgaa aaagcaggcg gtaaaacgca aactgcaccg gttgcaaccc
    15181 cgcaagaact ggccgattac gacgccatta tttttggtac acctacccgc tttggcaaca
    15241 tgtccggtca aatgcgtacc ttcctcgacc agacgggcgg cctgtgggct tccggcgcac
    15301 tatacggaaa actggcgagc gtctttagtt ccaccggtac tggcggcggt caggaacaaa
    15361 ccatcacatc cacctggacg acccttgcgc atcacggcat ggtgattgtc cccattggct
    15421 acgcagcgca ggaactattt gacgtttcac aggttcgcgg cggtacgccg tacggcgcaa
    15481 ccaccatcgc aggcggtgac ggttcacgcc agcccagcca ggaagaactg tctattgctc
    15541 gttatcaagg ggaatatgtc gcaggtctgg cagttaaact taatggctaa tcttcaacag
    15601 gaggatacgc atgccaactc aagaagcgaa agctcatcac gtcggtgaat gggcatctct
    15661 gcgcaatacg tcgccggaaa tagccgaggc cattttcgaa gtcgccgggt acgacgaaaa
    15721 aatggcggaa aagatttggg aagaaggtag tgatgaagtg ttagtcaaag cctttgctaa
    15781 gaccgataaa gattcgcttt tttggggcga acagaccatc gaacgtaaaa acgtttaacc
    15841 agcgactccc ccgcttctcg cgggggagtt ttctgttatt tcaccgcttc attcaacacg
    15901 ctatcaaact tatccatcgg gcagaaacca tcagcgtcta tcgggcaacc gcttaattcc
    15961 agcgtcacgc gctgcgcagg tgcctgcagg gttaacgcat cggcattacg taactgttcc
    16021 gcactctggt acacatattc aattttcatc aaatcgcgat tggctttgct gtcatgccaa
    16081 cgctggaaaa cgattttgcc gccaatcggc gtgcgttcgt tctggtcatg caactgatac
    16141 ggtttgaaat ccagcgccgt taacagagag gcaatgttgg agtcatgtcc aactaacacc
    16201 gtgatttttg gtgcactggt gcgatcggtg accagggctt tgtcgatata actgaccagc
    16261 ggtttcgcaa cattgcgcgc cacttccggt gaggtaaaca ggctgtcctg gtaaccgttt
    16321 ttcagcttcg acaacacctt ccactgctgg tcagatttga tttctcccca ggccacctga
    16381 tccatcggaa aaccttcgta gtattgcaaa gtaaacgcat ctaccagcga gttgccgact
    16441 ttcagcggcc cggaaacacc tggttcttgt tgatacttcg cgctaaaggt atttttgcca
    16501 tccaccagcg aacactgttg tttctcttta caggcagggg aatctttata gttaacgatt
    16561 ttttccagta gctggtagct gtcggtaagc tggagtttgc tgagctcttt ctccattgcc
    16621 gccaccgcct gttcactgaa tgcggcggaa tcatcggtga tcaccgggtt aaaggttggg
    16681 tccatggtgc ccattttttc ctggtgatgc acaggaatat cacaccccgg gaatgcgccg
    16741 gtaataaaga actgtgcggt cgcgacagta cgttgcagac tattggcata ggcataaacg
    16801 gtgtccggcg gcgggcattc ccccgatttt accatcccct gctctgccag ccattcacgc
    16861 atgtaatggc ccatatacac ttcgagcacg ccacctttgg tggtgagttg cccaccgggg
    16921 acgtcccatt cgggccattt attcggcgtc gactgttcca gcacactgcc attgttcgcc
    16981 agcggcgcac gtaagttatg gcggctcatc atgagcactt gctgtagctg atagccttcc
    17041 ggtacggttt gtgcctgagc atttgaagcg agtaaaacta tccctgccac agttgcggcg
    17101 attagcgttt tgttcattcc tggcacctct tttgttatca atcactaaag agtgtgacag
    17161 aaatatgact tttttccggg aacaggttcg caaaatggct tgttgaacgc ctgtttttct
    17221 atctacatct tcaaaaacag gcaaactatg cctgaatagc ccctgcctcg catacgccgc
    17281 tgatgattac accagtgggc tagacggaat atcagcggcg ttaccaatac agaggcaata
    17341 tttaccaata aaaaaagtgt gagactaata acaatggttt atagcacatg catttgtcat
    17401 taaattgcat aacgatttaa atagtcaagt tgcgcattca tttccttatt gtttaacttg
    17461 taagcctgag caaaaagttc aggagggagg taaacgtcat aatgattgcc agtgtttatt
    17521 aaataaccat actcaagatc ttcatctgtt gaaaaggatt cattccctaa ggagattgat
    17581 gccacattac tattaacaat aaataatgag caaatgctat ctgaaagaac agcaag
//
LOCUS       DAZUCE010000096        17510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZUCE010000096 DAZUCE010000000
VERSION     DAZUCE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.096"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..509
                     /gene="atoB"
                     /locus_tag="ACHY03_002707"
     CDS             <1..509
                     /gene="atoB"
                     /locus_tag="ACHY03_002707"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFS3905916.1"
                     /translation="EALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGL
                     TPLARIKSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVG
                     KSLGFDSEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGI
                     AMVIERLN"
     gene            complement(583..1359)
                     /gene="yfaP"
                     /locus_tag="ACHY03_002708"
     CDS             complement(583..1359)
                     /gene="yfaP"
                     /locus_tag="ACHY03_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFS3905917.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIHGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGRSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(1364..3013)
                     /gene="yfaQ"
                     /locus_tag="ACHY03_002709"
     CDS             complement(1364..3013)
                     /gene="yfaQ"
                     /locus_tag="ACHY03_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFS3905918.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKASTLPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TSVKPGVLNGRYRVTFANGNHITFVSHGETTLLTEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQAMAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(3014..7528)
                     /locus_tag="ACHY03_002710"
     CDS             complement(3014..7528)
                     /locus_tag="ACHY03_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFS3905919.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVRLEGASSNFISPQPGNIYIPLGKQEPGLYLVEAMVGGYR
                     ATTVVFVSNTVALSKVSGNELLVWTAGKKQGEAKPGAEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDSGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVSVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSLLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     AIVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDPRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPSVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWPGMQVK"
     gene            complement(7552..8175)
                     /gene="yfaT"
                     /locus_tag="ACHY03_002711"
     CDS             complement(7552..8175)
                     /gene="yfaT"
                     /locus_tag="ACHY03_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFS3905920.1"
                     /translation="MRHVLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKNNGFSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(8172..9860)
                     /gene="yfaA"
                     /locus_tag="ACHY03_002712"
     CDS             complement(8172..9860)
                     /gene="yfaA"
                     /locus_tag="ACHY03_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFS3905921.1"
                     /translation="MSGEKKAKGWQFYGLVGVGVVALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLNKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(10009..12636)
                     /gene="gyrA"
                     /locus_tag="ACHY03_002713"
     CDS             complement(10009..12636)
                     /gene="gyrA"
                     /locus_tag="ACHY03_002713"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFS3905922.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            12783..13505
                     /gene="ubiG"
                     /locus_tag="ACHY03_002714"
     CDS             12783..13505
                     /gene="ubiG"
                     /locus_tag="ACHY03_002714"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFS3905923.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(13646..17398)
                     /gene="yfaL"
                     /locus_tag="ACHY03_002715"
     CDS             complement(13646..17398)
                     /gene="yfaL"
                     /locus_tag="ACHY03_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFS3905924.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQVWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVSGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAIDLRVTNA
                     VFRNNIANDGKGGAIYTINNDIYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVILGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNEGSFAGTIEGAGQLTIAQNGSY
                     MLAGASSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYKDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVIDAGDGQVRLAND
                     NQYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDNQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     TGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSASDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     VMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFRGRWGDDGEW
                     MLGIVGGYSDNQGDSRSSMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDNWLQYA
                     WFNNDVSEQDDGADHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         3996 a         4892 c         4351 g         4271 t
ORIGIN      
        1 ctgaagcgtt aggcgcattg cgcccggcct tcgataaagc aggaacagtc accgccggga
       61 acgcgtctgg tattaacgac ggtgctgccg ctctggtgat tatggaagaa tctgcggcgc
      121 tggcagcagg ccttaccccc ctggctcgca ttaaaagtta tgccagcggt ggcgtgcccc
      181 ccgcattgat gggtatgggt ccagtacctg ccacgcaaaa agcgttacaa ctggcggggc
      241 tgcaactggc ggatattgat ctcattgagg ctaatgaagc atttgctgca cagttccttg
      301 ccgttgggaa atccctgggc tttgattctg agaaagtgaa tgtcaacggc ggggccatcg
      361 cgctcgggca tcctatcggt gccagtggtg ctcgtattct ggtcacacta ttacatgcca
      421 tgcaggcacg cgataaaacg ctggggctgg caacactgtg cattggtggc ggccagggaa
      481 ttgcgatggt gattgaacgg ttgaattaat caataaaaac acccgatagc gaaagttatc
      541 gggtgttttc ttgaacatcg acggcgaagg taaccccatt aatcaccagt caaaactttt
      601 caccagcgtc agctcgccag cattacgcat cggtacaata aatgtttcct gtttctcatt
      661 gaccgatcct tcatcggtaa tcagcgtcag ttgggcggtg gttaattccg tttcgctgcg
      721 cccaccatag tagttgatat acacctgata gcgcccgtga actggcgctg gcatggcgaa
      781 aatctcgggt ccataccccg tcgtgacatc catatccagt gcaccactgt ttttcagcac
      841 ggtgttaccg taccaggcgt gttcgccatc gggcgtgaca acgtgaagat cgaggtcggt
      901 attgtccgta tcccacgaga gaaccagccg taaacgtgca cgaatcgttc ctgtacccgg
      961 cgttgagtaa aactgcattt tttgtcggct tcgcccatcc gggctgatga cctgcacgct
     1021 gttgctgcct tcagtgaaaa tataagggcg tgcaaatgaa ccatcggatt caactctctg
     1081 cggcatactg gcaccgttga ccaccagccg gccctgctga actttacccg ccgcagcata
     1141 attctttatt ttgccgtgga tctgcgctga gatattttga tcatctgcca tgttgacgga
     1201 cgacgccggg taattgatcg attgtgaaaa actggcatct tcgccctctg ccggatgcca
     1261 gcctgagagc ggtgcgtcaa tctcgacttc ctgcacacct tcactgtgag cgacaggcga
     1321 aagcgccacc agtaacaacg gaagaaaaat ctttcgcata gcattagtcc attaataatt
     1381 gtcgggtcag cgtttcgata tagttctcat caagcccggt gggataacca tcaaaggcca
     1441 ggtgcagata ctcatgggtt aaatccagcc gatcctgaag cgtaaagaag ttacgaataa
     1501 acagccgttt ctgctgccga tcggtatagg ggaaacctga gactaaacga cagaccgcaa
     1561 acacgtctgg ttcgttgtac cccgtctcgc cttgtaatat acgccgccac tgcggcattt
     1621 ttttcgccag ccaggctttc gctttgggta ataactgaca ggttgagcgt ggcgctcccc
     1681 agcggctaag gctgttgtcg ggataagcaa acgcgagtat ctggtcgtaa cgctctccct
     1741 gcccggcctg ggccatggct tgccgccagg aaagcgtccc ttcggtagcg cgactgccgt
     1801 gataatgaac cggatcgccc gcatagatga gatcctgcgt ccaggcggtc atcgttcgcg
     1861 cccccgtggt agctggcgaa gcagaaacac gctgcgtggc gctgctgtcg gggatggtca
     1921 ggcaatcgcc ttcccgattg gcgttttgct gtaaaaaagt gcgtatcgcc acggtcatgg
     1981 ctttcgctgc ctcaggtggc gtacttttcg cctcgcgatc cagaactctg gcaacgtact
     2041 cttcacgatc gagatgcgac tgcaatttca gtttgccctt ttcggtcagt aaggtcgtct
     2101 caccgtggct gacaaacgta atgtggttac cattggcaaa tgtcacacga tagcgcccat
     2161 taagtacgcc gggttttact gacgtcgtac tcttttccgc tgtgattttc ttcagcggat
     2221 agcgggcaaa tagctccacc tctacacatt gcccactggc aacttgtgtc ggcactggca
     2281 aaacccgatt aagcaccgtc gcataacggg tcagtaccgt tttgctggtg ccactgccag
     2341 taacccacag cggtgtacca tcggttaacc agccggcaaa accaccctga cggatttcct
     2401 gtttatcatc ggcaaaccag ctccaggttt tcacccgtac ccgactaccg agcatggatg
     2461 cgacaccgat tttcgcctcg tccagcacca catccagtaa gacttcctga gctttgtttt
     2521 gcgcaggtaa cgtcgctaat gaatcaagca gcgatttaac cgttaccgag gtttctggct
     2581 tcagcgtagt aagagacgct aaccacgcag gtgcctgacg ctgttgccag tattgccccc
     2641 acacatctgc gccaatatgc aaccgctgcg gcgcaaaata gagcccacag gaacgtacca
     2701 gcgcggtatc gcgcgttatg ctctccccag cctgacaaca ataaacctct tccggggagt
     2761 ttccgcgaca ctggtagggc tgctccggtt gatgggtatc ctccagccag gcgtaaacat
     2821 acagtttcca caaactccct aacggcgtgg tgagcgagtc aggtaatgtc gaggctttgg
     2881 tcacgcctga tgacgacagc tgatacagtt gatcgtgctg cgcaccacgc agtgccagtt
     2941 gtaacggcgt ctcctccgca agcgttggca gagtaaccaa cgccaatagc cagacgattc
     3001 tgcgccagtt cacttatttc acctgcatcc cgggccattc gctccctgcc gcaacacttt
     3061 gctgcgcagg tgcataggaa cgcacataac gagcaggagg cagaacgaat tgcccttttt
     3121 gcgagaagcg cagcaaatgg cggaaagtga ccgttcccgt cagttctttc accggcacca
     3181 tataggccag ttcgcccatt tcattacgcg ctttttccag caattgcccc tgctgtttcg
     3241 cggcgttggg tttattgact gaaatgcccc atgttgtgcg ctcaacgtcg gctcccggcg
     3301 ggagcggtac ttccacctga ccatagcgca gaactgcatc ctgctcgctg gtaagcgtaa
     3361 tttcatcgag atacagcgca tcgctgtcaa tctcattgct ggtcaccggc tgcagagtaa
     3421 agctcatctc ttcttcaccg gggataagcc gatacaactg gcgttcaacg gtcaccggaa
     3481 tgttactttg ttgagccgtt tttgccggtt cacgccagcg gacctgcaca ttttgcggcg
     3541 ataattcgtc accaaaagag agaatgtccg gcacgccctg accaacccaa cgccagtatt
     3601 cgccccctcc agttaactta tgtttggccc atgcgcccgc aggcgcaggc aacacaaccg
     3661 aaggcattgt cgccatatat ttcgccagcc agttcatggc cagcgcgcgc tcaatagtag
     3721 attgctcagc ggtcaaacca cttaaaatag cagccgcctg ggtagcgtca ccaccgctat
     3781 tgagcagcac aaccgtgtgc gccagcggct gattgctgtt tgccgcttta tcccacgcat
     3841 attgctgaac ctgtggcatc accgtggact tcaactgcgc ttttttcagc aacgtcatgg
     3901 ttaagacgtt tgccaccgca tctgccagtg gagattccgg tgtatcgagg atcaggctgt
     3961 cattgatatc gcgggggtct tcctccgaga aatcttcagt tttagttccg cgccgggcga
     4021 tagcttcatc caaccctttc aacaacgttt tgcacggcag attcatctcc tgtgcccacg
     4081 ccagcaccag cgcccgatgc aataacggca tgttatctgc ctgctcggca taactgtcga
     4141 gcatatgctg ccagtattcc ggttgttgcg ttacgccgag cgcctggctg gcctgccagt
     4201 cggcgtacca tgcccatgcc gtaaggaagg cgtcaccatt gccatcttca ccccaccagg
     4261 taaagcgcgc tccgggcccc gccagttgca tcagccgcag acggttatcc tgaatcatct
     4321 gacgaatgtc gttagcggcg gcactttgat gatcggcaag cgaacgccag gcgagactga
     4381 gcgggatcag acggctaccg gtgttgatca ccccacccca cggttcatcg actatcgcat
     4441 caagattgtt gcggaaaatc tcctgcggcg tttcactact ttgtagccgg atattgctcg
     4501 cctgctcggg caacatcagc gcgttatcgc caccgccgag catgacattt ttctgctgtt
     4561 caacgggcca gctgttatcc acaaaagaca gttttgtgct aatgctgtcc tgcacttgcc
     4621 cattttgttg cagttctgca cttaacaagc cagattgctg aatgttctgc gtcagcgaaa
     4681 tataattcgc ccctttgtgc agcgtcagcg tctggcgcat ctcagcgcct gcaaatttag
     4741 tcaccagcgc caccggttcg ttatcctgct gactgaagat aaacagtcct gccgccggtt
     4801 tgtcgcccac gcgatacacc gttggcatac tccacttcat gtagagattt ttttccgaac
     4861 gcagataagc acgcccctgc ccgaccagcc cgtcgccgtt catcccacgc gcggtgatac
     4921 gccagcgggt taacgaatca ggcatcagga acgtgaaata cgctttgcct tgtttatcgg
     4981 ttgtgagtga cggcatccat gccgcggtat ccacctcttc acgccgtgga cgttcaagca
     5041 tttttactcg ccgctcgctg cggttagttg cgccaggcgc aaccggctcg ctggagagcg
     5101 cctggtcgta gctgataaac gacagactgg agctggtacg cacattgtta cgccccagcg
     5161 gatagaaaaa tttgccgata ttcggcgcga tttctggttg cagcgcgtag atcatttcat
     5221 cgaccacgcc taccgttagc tgcgcagaaa caggtttacc tttcagcaac gaggttaatt
     5281 cgacattgac cagttcacca ggctggtaat gggttttgtc cgttttcacc cggatatcca
     5341 gctggggaac ggcaactttg atcccggcat tctgaaaact gtactgaccg ttacgggtat
     5401 acagcaccga aaaagtgatg ttaggcgcaa aggaattgct cactgaaaca cgggcttcat
     5461 actgagtatc gtttaaacgt tgtaacgtca gccagtttgc cggatgcgat agcagcgact
     5521 gctgttccac gcgatcgcgt tccagcgtca ataatgcttc atcaattggc tccgggaagg
     5581 taatcagcat cttcgcggtt tcgccaggct ggtacagcgt tttatctgcc acgatatcta
     5641 ccgtaccagt atgcgacatg ctgcccttac cgctgactgc atggcttaac ccggcgagaa
     5701 ttaagccgtc tttatcgcgt aacgtcagat tgtagttgcc aggtttatcg aaattgacgg
     5761 taaaggattt gccgccagac ggtagctctc cgctatggct cgtgcggtct tcgagacgca
     5821 accattcata cgtaacagga acctgttttg aagattccag cgcggcataa cggaacacaa
     5881 ccgactcgcc gctattactg tattgtgcgg cggtacttaa agagtaatgc gccaggccgc
     5941 gttcaatgag gatctctttg gtggtggtga cgcgatacgc cgcgccgtca ctggcggaga
     6001 cggttaacaa ataacggctc ggtttatcgg cggcggggag atttaacgcc acatgaccgc
     6061 tatcgtcgga caccgtttca ctgccttcca gcgacacggg aaaacgtccg gcataacgca
     6121 aatcgttacc aaccattgat aattgctgag cgcgcaaact taactgcacg cgggcatctt
     6181 ttaccggctc accatccggg tagagaagtt gcagtttgcc gctgaccgct tcgccagttt
     6241 tgaactcttt tttgtcgaga gctaaaccaa tctcgaaatg tggcttgatg tagtttgcca
     6301 cgcgaaaact gctgctatag acctgattgc ggtaagcaag acgtaactca taacctccgg
     6361 caacggcatt ttctggcagg cggaaacttc cctgcccgcc attgcgcgca tccagcgtga
     6421 catcgacggt ttgcaacaga ctgccgttag cgtccagcac cgaaagcttc gccggggcgc
     6481 tgacgatggg ggatgaatgc aacggatcgt ggaactcgcg gcccatcact ttaacatcga
     6541 cacgatcgcc tgcgcgatat agcgggcgat cggtaaaaat atacaagcgg gtgttgtaga
     6601 tttcgctttc gtagaagaag ttctcggaga caaaaacgcc gccttcagca tccttaccca
     6661 gaatgtatga acgttctggc gatatatgtt gtaactgcaa ggtaccgctg tcatcggtca
     6721 caccgcgagt catcacgcca agaccgtctg tccacaaaat ttctgctccc ggcttcgctt
     6781 ccccctgctt ttttccagcg gtccacacca gaagttcgtt acccgacact ttactcaacg
     6841 ccacggtatt ggagacaaat accaccgttg ttgcccgata accgccgacc atcgcttcga
     6901 cgagatacag ccccggctct tgtttgccga gagggatata gatgttgccc ggctgcggcg
     6961 agatgaagtt actggatgca ccttccagtc ttacgccttg ctgcggctcg accggtttag
     7021 cctgccataa tgggtaacgg aactgttcca ccagtggata ttttttcagc ggtgaaaact
     7081 ggttgttctg tacataacgg gaaggtttga taatggcatt gccgagctgt aattcgggta
     7141 acgcctgagt cacattctgc cgtgactgag aagagaaagt acgctgcatc acgcggcgag
     7201 atttgccgta ccagttatcc cacagccagg tcagcgtatt gttcagcccg tcgcccagat
     7261 attgcggttg caccacaatg cgatgcaggt ttttctgctg gcgcaaaaat gccatcgggt
     7321 caggaatacg atacaggcga acgtccacgc cgccgtactc ttccatctga tagcgccgat
     7381 aatcacgccc cggcgcttcc agtcgcactt tcgcctcttc actgctgcta aaactgctgt
     7441 cagcaagcaa aaagaatgtt ccccctgcgg gcggcgcata gttactggaa ggaagcgaat
     7501 catcagcatt agcaagccct gttcccacaa gagacaggga caagcacgct gctatcgcgc
     7561 cagaaaattt aaacgataaa tgccaatgaa attgggattg gaatcgttgg gtatccatcg
     7621 ggtgtccttc catgtcataa gttgttgcag actgactgcg cgcattccgt tgtcagtttt
     7681 cgtggcgctt ccggtgtggt agatgacgta acgccccatc cagaccatta agtgctgggc
     7741 atcgccctga tcgaaaaaaa tcatatcgcc aggcagcgcc tggtttatgt cctggccaat
     7801 aaattggctg ttgtactgaa tcaaattaat cgcggttacg taggggccgg ttttcccgtt
     7861 cccctgattc cagttttgcg ccagttgccg ctgttcaggt gttagcgtca tctctggcgg
     7921 caaatactgg ttagagaaac cgttattttt tagccattta ctgtcgtgaa ctttcagcgc
     7981 ctcgttcgct gcaaatcgca ccaggcccgc acaatcctgc tgataccagc gtggacttgg
     8041 cccctggcgg agctgttcct gagcaatgcg cacaaaccag gcgcgaaaca gcccggattg
     8101 ttcgacattc agcatttcgc tatgggcaac aacacaacac aaccagcaaa tcagcgccag
     8161 caggacgtgc ctcatagcgg ctgccaggtt atgggtagcc actgccaggc ggcaccgggt
     8221 tccatctggg ccagcttcat gacataacgc ggttgttgag ataaagcgtc cagcttcggc
     8281 attaataaag tttgtgcggc attataaaaa accggttcga gattcttcgg cagactggtc
     8341 aacgtttcgt tacgcagcag tttcgccacg ccttgtggat tgatatagag cggaacgatg
     8401 ccatcagtgg gtattacatc caccattgcc gggcgggttt tattcagtgt ttgcagggcg
     8461 ttattaacca gcgtgtcatc gagggagaaa agcagcgttt tgttttgcat cgccagcgac
     8521 acgcggaaaa aataatccga cattaattga tcgggttgcg ccgcctgcgc tttcggatac
     8581 tgaccgtatc gggaactcac ttcgcgacgc cagatttgcg cttcgccctg ctgagtctgg
     8641 cttaccggca aaacaccttc tggcgctttg ctttcgtgcg caccaatatt ttgcgtaaac
     8701 agtttccctg gcaattgcgc ctgttcggca gtgccatcaa actgaccgac aaacagcggg
     8761 gtttgcaatt ttgagtcttc ataccagcac agccccgcgg caccgtctaa cgccccattc
     8821 aacttgtcgt tttcctggct gatgtgcgaa agcatctctt cggcaatacc gtgtgaatac
     8881 ggcaccgcca cacagaaact ggccccggca ggcatactgt tccagaccgg cgtaaaatcg
     8941 aaactggcat ctacgctggc ggattcatca tttaacgcca caaaactgtg ccagccgtcg
     9001 ttacccattt cgaagcgtac gccagcaaaa gaaggcatta atcgctggta gccaaacccc
     9061 agcaacctgg cgctgactac gatacgctgg cgtactggcg ttttttcggc agtacgctct
     9121 tccaggccaa agctggcttg ccagcgtttt ttgccgctca acaaatcact tgcgatcgcc
     9181 gtggcttccg tatcctgctg gtcatcttta aacagcatat ccgtgctgga aaacaccaac
     9241 atcttgtcct gatatgtcgc gaacatcagg acgttattgc cgttatagcg caactgataa
     9301 acaggaatag tttcactatt tatcttgatg ctactgattt ccgttttgtt taactggctg
     9361 tcgctggtag cggcaaataa caatggctcc agcagtttgc ttaaaccact gcgttggatc
     9421 agcaccatgt aatgtgaaag atggccttgt ttatcgtgcc atagcgccgc ctgcgcgggc
     9481 tgatctaaga gtgacgaaaa gagcttatct ttcagcgtga gatcgtgttc atagacaatg
     9541 cgacgaatgc tgccttcaat gcccagacga tcggcatgat tctgataata gaaaacgaaa
     9601 tcttcactca gaacatcgtg gagaaacgga atggtgagga gatctttggg aagctggctc
     9661 agagagtcgc tgtcgagaaa gaggtccggc tcattgagat cgatttgcag attgttgtgc
     9721 accaccagcg gcgacaacgt tttttctggc ccactgccag catattgcaa cgcccagacg
     9781 cccgcggaaa ggagcgcaac aacaccgaca cctacaaggc cataaaactg ccagcctttc
     9841 gccttttttt caccactcat tgccacattc cttgtgtata gccagccatt ttttacgggc
     9901 acagccaaac tttaccgtgc cctaatacga caaaagccca gactttgcag cctggacttt
     9961 tcaattcaaa caagggagat agctcccttt tggcatgaag aagtaaaatt attcttcttc
    10021 tggctcgtcg tcaacgtcca cttccggagc gatttcatcg tccccttccg cggcactgcc
    10081 gtcgatggta tccagatctt cctcgtcaac cggttcagca acacgttgca gacccactac
    10141 gttttcatct tccgcagtac ggatgaggat cacgccctgg gtgttacggc ccacgatgct
    10201 gatttctgaa acgcgagtac gtaccagcgt accggcatcg gtgatcatca tgatctggtc
    10261 gcagtcatct acctgtaccg cgccaaccac taaaccgtta cgttcggtaa ccttgatgga
    10321 gataacccct ttcgtcgcac gcgacttagt cgggtattcc gccactgcgg tacgtttacc
    10381 ataaccgttc tgcgttgcgg tgaggattgc gccatcgcca cgaggcacga tcagagagac
    10441 gactttatca ccttcgccta aacgaatacc gcgaacaccg gtggtgttgc agcccatcgc
    10501 acggacagaa gactctttaa agcgcaccac tttaccttcg gcggagaaca gcattacttc
    10561 gtcttcgccg ctggtcaggt caacgccgat cagctcatcg ccttcaacca gtttgatcgc
    10621 cactttaccg gcggtacgca gacggttgaa ctcggtgagg acggttttct tcacagtccc
    10681 gttagcggtc gccatgaaga ctttcacgcc ttcttcaaac tcggtcaccg gcaggatcgc
    10741 ggtgatacgt tcgtcctgct ccagcggcag caggttgacg atcggacgac cgcgcgcgcc
    10801 acgagtggcc tccggcaact ggtaaacttt catcgaatag acgcgaccac ggctggagaa
    10861 gcacagaata tggtcgtgcg tgttcgccac cagcagtcgg tcgataaagt cttcttcttt
    10921 aatacgtgcg gcagatttac ctttcccgcc acgacgctgc gcttcgtatt cagaaagcgg
    10981 ctgatattta acgtagccct ggtgagagag cgtcacgacc acatcttcct gggtgatcag
    11041 atcttccagg ttgatgtctg cgctgttggc ggtgatttca gtacgacgtt tgtcaccgaa
    11101 ctgttcacga accagctcca gctcttcacg gatcacttcc atcagacgat cggcgctacc
    11161 aagaatacgc agcagttccg cgatctgatc cagcagctct ttgtattcgt cgagcagttt
    11221 ttcgtgctca aggccggtca gtttctgcaa acgcagatcc agaatcgcct gagcttgctg
    11281 ttcggtcagg tagtacagac catcacgcac gccgaactct ggctccagcc attccggacg
    11341 cgcagcatcg tcgccagcac gttcgagcat cgcggcaacg ttgcccagct gccacggatt
    11401 agccaccagc gcagttttcg cttctgcagg cgtcggcgca tgacggatca gttcgatgat
    11461 cgggtcgatg ttcgccagcg ccacggctaa tgcttcaagg atatgagcac gatcgcgagc
    11521 tttacgcagt tcgaaaatag tacgacgggt caccacttca cggcggtgac gaacaaacgc
    11581 cgcgatgatg tctttcaggt tcatgatctt tggctgacca tggtgcaatg ccaccatgtt
    11641 gataccgaaa gaaacctgca actgggtctg ggagtagagg ttgttgagca caacttcacc
    11701 gaccgcatcg cgtttcactt caatcacgat gcgcataccg tctttgtcag actcgtcacg
    11761 cagcgcgctg atgccttcca cgcgtttttc ttttaccagt tccgcaatct tctcgatcag
    11821 gcgcgctttg tttacctgat acggaatttc gtggacgata atggtttcac gtccggtttt
    11881 ggcgtcaact tccacttcag cacgggcgcg gatatatacc ttgccgcgac cggtacggta
    11941 agcttcttca ataccgcgac gaccgttaat gattgccgcc gtcgggaagt ccggccccgg
    12001 gatgtgttcc atcagccctt caatgctgat gtcttcatca tcgatatacg ccagacagcc
    12061 gttgatgact tccgtcaggt tgtgcggcgg gatgttggtt gccataccta cggcgatacc
    12121 ggaagaaccg ttcaccagca ggttaggaat tttggttggc atgacgtcgg gaattttttc
    12181 cgtgccgtca tagttatcaa cgaaatcgac cgtctctttt tcgagatcgg ccatcagttc
    12241 atgggcaatt ttcgccagac ggatttccgt ataacgcatt gccgccgcag agtcgccgtc
    12301 gatggaaccg aagttaccct gaccgtctac cagcatgtaa cgcagcgaga atggctgcgc
    12361 catacggacg atcgtgtcat aaaccgccga gtcaccatgg ggatggtatt taccgattac
    12421 gtcaccaacg acacgggcag attttttata ggctttgttc cagtcattgc ctagtacgtt
    12481 catggcgtaa agtacgcgac ggtgtaccgg cttcaggcca tctcggacat ctggcagcgc
    12541 acggccaaca atgaccgaca tcgcataatc cagatatgag ctcttcagct cttcctcaat
    12601 gttgaccggt gtaatttctc tcgcaaggtc gctcatctaa ccgctatccc tctactgtat
    12661 cccggattca aaggtcgcaa attataacac agccgcgcag tttgaggtaa acctatacgc
    12721 tttattcaca tccaatgcct gatatactcg tttgtcttgc caattacgga gtagaagtgc
    12781 caatgaatgc cgaaaaatcg ccggtaaacc ataacgtaga ccacgaagag atcgctaaat
    12841 ttgaagccgt cgcctcccgc tggtgggatc tggaaggtga gttcaaaccg ctgcaccgca
    12901 ttaacccgct gcgtctgggc tatattgccg agcgtgctgg cggtttattc ggcaaaaagg
    12961 tgctcgatgt cggttgcggc ggcggcattt tggccgagag tatggcgcgc gaaggcgcga
    13021 cggtgaccgg tctggatatg ggctttgagc cattgcaggt ggcaaagctg cacgcgctgg
    13081 aaagcggcat tcaggtggat tacgtgcagg aaaccgtgga agagcacgcg gcaaaacatg
    13141 ccgggcagta tgatgtggtg acctgcatgg agatgctgga gcacgtcccc gatccgcagt
    13201 cagtggtcag agcctgtgcg caactggtga aaccgggcgg cgatgtcttt ttctcgacgc
    13261 ttaaccgcaa cggcaagtca tggctgatgg cggtagtcgg cgcggaatat attttgcgca
    13321 tggtgcctaa aggtacacat gatgtgaaga agtttattaa accggcagaa ctgctgggct
    13381 gggtagatca gacaagcctt aaagagcgac atataaccgg gctacattac aacccgatca
    13441 ctaatacttt taaactcggc cccggcgtgg atgtgaacta tatgctgcac acgcagaata
    13501 agtgaggttg ttgtttggat tcatccggca accgcgccaa tgcctgatgc gacgcttgcc
    13561 gcgtcttatc aggcctacaa atgctctccg taggccagat aaggcgttta cgccgcatcc
    13621 ggcaatagtg atggtcagac agcgattacc atttcaccgt catcgacaaa aaccctgccg
    13681 tctgggcaaa atcatcactc cctttctgcc acgccacgct accacgcagg gacactcgct
    13741 gactgatatt gcccgtgact cccactttta tttcaccccg ttgcttcacc gcatcgtcac
    13801 tgatagtgct gccatcttct tcaatttccg tcgaatgggg atcgtgataa taattcagat
    13861 ctaatgttgg tatgacatga acagcggtac gccattcact gtgtaaaccc agccgcgtct
    13921 gaatatcatc accctgcgat tgtgacacgc gcgcacggtt agcggcggta aaatcatcct
    13981 gctgcacgcc ctgataaatc acctgcgcct gcggttcaat caccacacca cgccccggta
    14041 accattgata cccggcttcc agcgaggcga taatccccga cgaatggtaa tgatccgcgc
    14101 catcgtcttg ctcggaaaca tcattgttaa accacgcgta ttgcagccag ttatccagcc
    14161 aggccccctg cttctgatta ccgtgctgaa accagcttga ggtcagccca accgcataac
    14221 cgtggttctg gttatcggcg cgagttccgg tcatactcga gcggctgtcg ccctggttat
    14281 cgctgtagcc acctacaatc ccaagcatcc actcgccatc atcgccccag cgccccctaa
    14341 acaggtcacc gctaagctgc accgtagaag tgtcttcatg ctgagccagt tgccctgccg
    14401 ccgtgtaatg ataacgtccg ccgataacac gtaaattcag cgtctgacca tcgccaccag
    14461 cgtgatcgcg tcgctccatc acaaacgcct gatttgccgc ccgcaggtta ttgagataac
    14521 cgccaacttt ggcattcaac accggctggt aagcaggcgt aggttccggg tcgggtgttg
    14581 gatcaggatc cggcgtggga tcaggatcgg gagtcgggtc tggatcaggt ggcgatggcg
    14641 gcgttacttc ttgcgatcgc agataccagt cgttgttatc ttccaccagc gtgtagtcat
    14701 acgctcccat attgacgtag ccgctgcctg ccagactgaa ctgcgcattg ttttgaaact
    14761 gcgtgggatc agctgcgaaa tcaaccactt taatgcctgt cgatgtcggc tcaccaatcc
    14821 ctgtaatgga gttaaccacc acagttgtgt tgccagcagt attaccgttc aacaccaact
    14881 ggtcgcttgc tgagtcatcg ccgtttaatt cgctgtcgaa cagtaacgta ccgccgccgg
    14941 tgtaatcacc gtttaccgtc agcgtatcgc cagcgacgcc attttgcagg ctgaccgcac
    15001 cggtcgtatt ggtcagattg ccattaaccg tcacgttgga aagataagtc aggctgtcag
    15061 tttcaaggtt ggtattaagc tcacctgtca gcgtcacgcc atcggtggca ttcaccagcg
    15121 tcccatcgcc gttaaacagt gaggcattaa gggcgacgga agtatcctgc ccggtcaggc
    15181 gcaaaaccgt accatcgcta atgtcgatag tgccgctgga agtagcatca attgactgaa
    15241 tatcctgatc cgcgccagta acgaacgtcg ccccatcacc gacccacagt gacgaagcat
    15301 aaggaaagat atccgcaaca tcgcctttca gggtgccttc ttcgacacgt accgccgact
    15361 gcgttgtacc gctggcggtc agctccagcg tacccgcccc cgttttcgtc aatgtgctac
    15421 cctcatcctg atgctgcccg ctgctgtcag ccatcagtgc gccccactgc gtgtctaccc
    15481 ccgcatcaac tgccacttca ccgtcggcgc gcatttcaat atcacgccca tgctcagtcg
    15541 ttgtagagcg agtatcgaca ttggcagtaa tctccatcac gctttcttgt tgattatcgg
    15601 taaagataac ctggcggttg taatgggtat ctccaagctg cgagttgtcg ctcaccatca
    15661 gcgtaccgga ggcgatttgc gttgtgccga ggtattgatt gtcatttgcc aggcggacct
    15721 gcccgtcacc cgcatcgatc accacgtaga cgccatcttt cccgtcaccg ccgatatgca
    15781 tatcgtttcc gcctgcaaga tctaccacat cctgactgcc gataaccgtt ccgctgctgc
    15841 cgctgacttc aaggccatct ttgtatgatg taccgctctg ccaggtggag aaatcagaga
    15901 gatcgagcac accgccgttt aaaacgatcg actgcggatc gtcctggaga gcggcaagat
    15961 ctgccgcgtc gccttccagc gaaagcaccg caccatcatc aacgactata tcgccggtta
    16021 gcgccatcga cgacgctccc gccagcatat agctgccgtt ttgcgcaatg gtgagctgac
    16081 cagcaccttc gatggtgcca gcaaaactgc cttcattaac agtaacattg ccaccagcat
    16141 cgatatttaa agtaccattc tgaaagcccg tcaatgagtg cacaaaagtt tgttgggtgg
    16201 agccaacatt tagctctgcc tgattctggt acttatcaat actccctatc gtcagaccgt
    16261 agcagtcttg cggatcgtcc tggcaatgcg tatctccgac attcatcagg gagttgctgc
    16321 ggcccaggat aacttcaccg ttttcaatct gcatctcacc agtaaagtca ttgttatctg
    16381 cattaagtac cagatctccg gaacctgttt tggtgatcaa cccggtacca gcaatagagt
    16441 caacagctcc gtcattctct gtattgccga taaccagcgt ttttccgtcg gcaatatcaa
    16501 aggtaacttc acttaagccg agatacataa agccacccgc cgcagtggaa ggaccatctc
    16561 catagcctgc tgcgctattg ttctcatcga ctaacacgcc tccgttctgg ctgtagctgt
    16621 catcaacaga aatatcaata agatagggag ccgtcgcgct attggtatat atcgccccgc
    16681 cataaccttc ggcagtgtta tttgtaaagg cagtgttatt tattatcgta taacctgaag
    16741 gatgcttgct gtcgctatta ttatctgtga catcgattgc gccgccatcg ccatcactgt
    16801 aacttgttga tgtatatgcc tggttattgt taaaaacatc atcacttaaa tagatatcat
    16861 tattgatggt ataaattgca ccacctttgc cgtcattagc gatgttattg cgaaacacgg
    16921 cgttagtgac acgtaaatcg atggcaccgg tatcgttagt accagaagaa tagattgcgc
    16981 caccatagcc gcctgcgacg ttaccagaaa aaataacatc cgtaagattc agcgttgagt
    17041 tttctttagc aaatattgcc ccgccgttat tatattctcc tgaaacggtg ttattggcaa
    17101 acagagtcat tccagtttca ttttctggtg ataatgtaaa ttccgctcct tgttgcaaaa
    17161 atacggcccc accgctggca ttattggtca tatccgaaaa aaccagccat tgcccatcgg
    17221 cgatactcca gacctgcgta atgcctgaaa ggctttgcct gctggcgtga caactcgctt
    17281 tgatatcata tccctggcat aaatcaatgg ccgccgcgac accgttagca gagaaaagag
    17341 atgcaatcat tgacgggagt agagataaat actccttgcg tagaaagata atccgcatat
    17401 taatctgacc atcattttat ataagacggc atatttaaac gcattataca atatgggtta
    17461 tctttctttt gtaaattgtc caacccgatg caatagctat tttgttatta
//
LOCUS       DAZUCE010000097        17448 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZUCE010000097 DAZUCE010000000
VERSION     DAZUCE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.097"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..159)
                     /locus_tag="ACHY03_002716"
     CDS             complement(<1..159)
                     /locus_tag="ACHY03_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFS3905925.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIE"
     gene            complement(286..1017)
                     /gene="rlmB"
                     /locus_tag="ACHY03_002717"
     CDS             complement(286..1017)
                     /gene="rlmB"
                     /locus_tag="ACHY03_002717"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFS3905926.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1198..3639)
                     /gene="rnr"
                     /locus_tag="ACHY03_002718"
     CDS             complement(1198..3639)
                     /gene="rnr"
                     /locus_tag="ACHY03_002718"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFS3905927.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3678..4103)
                     /gene="nsrR"
                     /locus_tag="ACHY03_002719"
     CDS             complement(3678..4103)
                     /gene="nsrR"
                     /locus_tag="ACHY03_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFS3905928.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4308..5606)
                     /gene="purA"
                     /locus_tag="ACHY03_002720"
     CDS             complement(4308..5606)
                     /gene="purA"
                     /locus_tag="ACHY03_002720"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFS3905929.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5710..5907)
                     /gene="yjeT"
                     /locus_tag="ACHY03_002721"
     CDS             complement(5710..5907)
                     /gene="yjeT"
                     /locus_tag="ACHY03_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFS3905930.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5989..6993)
                     /gene="hflC"
                     /locus_tag="ACHY03_002722"
     CDS             complement(5989..6993)
                     /gene="hflC"
                     /locus_tag="ACHY03_002722"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFS3905931.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6996..8255)
                     /gene="hflK"
                     /locus_tag="ACHY03_002723"
     CDS             complement(6996..8255)
                     /gene="hflK"
                     /locus_tag="ACHY03_002723"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFS3905932.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8341..9621)
                     /gene="hflX"
                     /locus_tag="ACHY03_002724"
     CDS             complement(8341..9621)
                     /gene="hflX"
                     /locus_tag="ACHY03_002724"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFS3905933.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9698..10006)
                     /gene="hfq"
                     /locus_tag="ACHY03_002725"
     CDS             complement(9698..10006)
                     /gene="hfq"
                     /locus_tag="ACHY03_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFS3905934.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10092..11042)
                     /gene="miaA"
                     /locus_tag="ACHY03_002726"
     CDS             complement(10092..11042)
                     /gene="miaA"
                     /locus_tag="ACHY03_002726"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFS3905935.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(11035..12882)
                     /gene="mutL"
                     /locus_tag="ACHY03_002727"
     CDS             complement(11035..12882)
                     /gene="mutL"
                     /locus_tag="ACHY03_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFS3905936.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIEFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12892..14229)
                     /gene="amiB"
                     /locus_tag="ACHY03_002728"
     CDS             complement(12892..14229)
                     /gene="amiB"
                     /locus_tag="ACHY03_002728"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFS3905937.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRSLPN"
     gene            complement(14248..14709)
                     /gene="tsaE"
                     /locus_tag="ACHY03_002729"
     CDS             complement(14248..14709)
                     /gene="tsaE"
                     /locus_tag="ACHY03_002729"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFS3905938.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14681..16228)
                     /gene="nnr"
                     /locus_tag="ACHY03_002730"
     CDS             complement(14681..16228)
                     /gene="nnr"
                     /locus_tag="ACHY03_002730"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFS3905939.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKALGIEVTLLAQDSDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     SAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGEAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16227..17366
                     /gene="queG"
                     /locus_tag="ACHY03_002731"
     CDS             16227..17366
                     /gene="queG"
                     /locus_tag="ACHY03_002731"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFS3905940.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17349..17402)
                     /locus_tag="ACHY03_002732"
     CDS             complement(17349..17402)
                     /locus_tag="ACHY03_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905941.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3969 a         4963 c         4371 g         4145 t
ORIGIN      
        1 ttcgataatc atttggcggg aagtaaaaag aatattgatt tgcaaatcgc cataatcatt
       61 cattttaata attaatgcgt tctcgctatt tgttacatca atattttgtt caggtacagt
      121 ttgcagcgcc gtcgccagcg ccaacggatt ccatgtcata tattattcca tatagattaa
      181 gtttaaatat taataaaatg aatatttgca gtacgtaatt atcttaccag ctatagacaa
      241 aaaaaaacca tccaaatctg gatggctttt cataattcag aggaactagc tgcgctgacg
      301 aaccgcttca aataagcaaa ttccggttgc aaccgaaacg ttcagagaag aaacacttcc
      361 tgccattggg atgctgatca actcatcgca atgttcacgg gtcaggcgac gcataccttc
      421 accttccgcg cccatcacca gcgccaggcg tccggtcatt ttgctctgat agagtgtatg
      481 atccgcctca cctgccgtac cgacgatcca gatattctct tcctgcaaca tacgcatggt
      541 gcgcgcaagg ttagtcaccc gaatcagtgg aacgctttct gccgcgccgc aggctacttt
      601 tttcgccgtg gcgttgagct gtgcggagcg atctttcggc acaatcaccg cgtgaacgcc
      661 agcagcgtcc gcgctacgca ggcacgcgcc gaggttgtgc ggatcggtta caccgtcgag
      721 gatcagcagg aacggttgat cgagcgaagc gatcagatcc ggcagatcgt tttcctgata
      781 ctgacgtcct ggcttcacgc gggcgataat gccctgatgc acggcaccgt cgcttttctc
      841 gtcgagatat tggcggtttg ccaactggat aaccacgccc tgagactcaa gggcgtgaat
      901 cagcggtaac agacgtttat cttcacggcc ttttaaaata aagacttcct gaaaacgttc
      961 aggggcgcgc tccagcaggg cctgcactgc gtggatgccg taaatcattt cgctcattaa
     1021 tgtactcgtt gtttacgttt tgcctgatgc actacgttta tccggtctac atgatccctg
     1081 caatatattg aatttgcgtg attatgtagg ccggataagg cgttcacgcc gcatccggca
     1141 tgaacaaagc gcattttgtc agcaatctaa ccctcttctg tttgaagagg gtaatgatca
     1201 ctctgccact tttttcttcg ccgcacgctt cgctttggtc gctgcagcta ttttctgcgt
     1261 tttcgccgat ggctttttcg cttttctcgc gtctttcttc gctgctttcg gcttcgtttt
     1321 tttctcaccg cggaaggcgc tgtctggctc aaagtttacc tttttaccga cctgacgacg
     1381 cttgccgcct tttttacctg catcgccttt tttcgctttc tcgcgcgccg ttttaccgac
     1441 gttgcgcggt gcgcgttcgc tggagatcag gctaaagtcg attttgcgct cgtccatatt
     1501 aaccgcttcg acgcgaactt ccacgcgatc gcccaggcga taagtctggc cgctggattc
     1561 acccatcagg cgttgcccta cctggtcaaa gcgatagtag tcattgtcca gcgaagagac
     1621 atggaccagc ccatcaatga acaagtcgtc cagacggacg aagaagccaa agccagtgac
     1681 gctggaaatt acgcctttaa agacgttacc tacctggtcg agcatgaagt cacacttcag
     1741 ccagtcagcc acatcgcgcg ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg
     1801 acccagttgc agcatctctt ccatcgaata atggtagcca ccggtttcag tggtgttgcc
     1861 ctgatgcccc tgctctttcg ccaacagata cttaatggcg cggtgcagcg tcaggtcagg
     1921 ataacgacga atcggcgaag taaagtgcgc ataggactgc aatgccaagc caaagtgacc
     1981 acggttttcc ggatcgtaaa tcgcctgttt catcgagcgc agcagcatgg tttgcagcat
     2041 ttctgcatca ggacggtcgg caaccgactc cagcagctcc gcgtagtcgc gcggttccgg
     2101 cttgttaccg cccggcagtt ccagccccag ctctgccagc actgaacgga aagaggtaat
     2161 cgcttcggtg ctcggcttgt cgtgaatacg gaacagtgcc ggttctttgg ctttctcaac
     2221 gaaacgcgcc gccgagatat tcgccagaat catgcactct tcaattaact tgtgcgcgtc
     2281 gttacgctga gtctgttcga tacgctcaat acggcgttca gcgttgaaaa taaacttcgc
     2341 ttcttcgctc tcaaatgaga tcccgccacg ttcttcacgg gctttatcca gcactttata
     2401 gaggttatgc aactcttcga gatgcttaac cagcggagcg tactgctcgc gcagatcctg
     2461 atcgccctgc agaatatgcc agactttggt gtaggtcaga cgcgcgtgag agctcatcac
     2521 cgcttcgtag aatttgtagc ccgtcaggcg gcctttcgac gaaaccgtca tctcgcacac
     2581 catacacagg cggtctacct gcggattgag cgagcacaga ccgttagaga gcacttccgg
     2641 cagcatcggg ataacctgcg aagggaagta caccgacgta ccacggttac gcgcttctct
     2701 gtccagcggc gttggcggac gcacatagta gctgacgtca gcaatcgcga cccataaacg
     2761 ccagccgccg ccgcgttttt tctcgcagta aactgcatcg tcaaagtcac gggcgtcttc
     2821 gccatcaatg gtgaccagcg gtaaatcgcg cagatcaaca cggcccgctt ttgcttcttc
     2881 cggcacttct tctttcagcc cggcaacctg ttgctcaaca gcctgcggcc agatgtacgg
     2941 aatttcatgg gtacgcagag cgatatcaac cgccatgccg gtgcccatat tatcgccaag
     3001 gacttcgacg attttaccca ccgctttggt gcggcgagtc ggacgctgag tcagttcgac
     3061 tacgaccaca aagcccatcc gcgcgcccat gatctgatcg ggcgggatta agatatcgaa
     3121 gctcagacgg ctatcgtcag gaaccacaaa gccgacgccc gcttcggtaa agtagcgacc
     3181 aacaatctgg ctggtttttg gcaccagtac gcggacaata cgcgcttcac gacgaccttt
     3241 acggtcagcg cccagcggct gcgccagcac ctgatcgcca tgaatgcagg ttttcatctg
     3301 ctcgctggag agatacaaat catctttacg cccttcaacc cgcagaaagc cgtagccatc
     3361 acggtggcca ataacggtac ctttcactag gtcgaggcgt tccggcagcg catagcactg
     3421 acgacgagtg aagaccagtt gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc
     3481 ttcaagctgc tcttcgcctt caatgtgcag ttctaccgcc agctcatcac ggctggccgg
     3541 tttttcacgt ttggttaaat gttcgaggat aaattcccga ctagggatgg gattcgcgta
     3601 tttttcagct tcgcgttcct ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc
     3661 tctgatgaag attttcgtca ctccaccagc aataatttat aaagcggttg attctcttca
     3721 accaaatcgg caagcgtgta gttatccagt tccgtaagaa aactttgcac ggccttagaa
     3781 agcgcctgtt tcaaccgaca ggcaggtgta atgtggcaaa actcactgct gcaattcacc
     3841 agcgataagg gctccagctc gcgcaccaca tcaccaatac gtatcgcact cgccggttta
     3901 cccaggcgaa tgccgccatt ttttccacga acagcagtca cgtagccggc acgactaagt
     3961 tgattgatta ttttgaccat atgattacgg gagacgccgt agacgtcagt cacttcagaa
     4021 atactggtca tccgcccttc tggcaatgac gccatgtaga tcagcgcacg taatccgtaa
     4081 tcagtgaaac tcgttaactg cacatcaacc tcaaaaggga aatcgggaaa aaatacattt
     4141 atattgatga taaaccagcc acaagctgtg tcgctaattt atttcagttc ggggaaggaa
     4201 aaaagcgagg atttaacact gtgtcggata gcgtaatggc ttaaccgaca cagcaatgta
     4261 tcacactgtt agcccggcag gcaaaatatc tgccaggcgt accagaatta cgcgtcgaac
     4321 gggtcgcgca gaatcatggt ttcagtacga tccggaccgg tagagatgat atcgatcggc
     4381 acaccggtca gctcttcaat acgtttgatg tagttcagcg ccgcctgcgg cagaccgcta
     4441 cgatctttaa cgccgaaggt ggattcagac cagcccggca tggtttcgta aatcggctct
     4501 acacctttcc agtcgtcagc tgccagcgga gtggtagtca cttcacgacc atccggcata
     4561 cggtaagcca cgcagagttt aacctctttc aggccatcca gaacgtccag tttagtcagg
     4621 cagaagccag acagggagtt caactgtacc gcacgacgaa cggcaacggt gtccaaccag
     4681 ccggtacgac gacgacgacc cgtagttgcg ccgaattcgt taccctgctt gcagaggaac
     4741 tcgccagttt catcaaacag ttcagtcggg aatggacctg cacccacacg agtggagtaa
     4801 gctttgagaa tacccagaac gtaatcaaca taacgcgggc ccaggccgga accggtcgcc
     4861 acgccaccag cagtggtgtt ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc
     4921 agcagcgtac cctgcgcacc ttcgaacatg acgaaatcgc cacgctgacg cgcctggtcg
     4981 agcaggtcag aaacgtcaac caccatagaa gtcaggatgt cggcaacagc catcgtatca
     5041 tccagaactt tctggtaatc aaccgcttca gctttgtagt agttaaccaa ctggaagttg
     5101 tgatattcca tcacttcttt cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca
     5161 acacgcagac cgcgacgtgc cactttatct tcataagcag gcccgatacc acgaccggtg
     5221 gtgccgatcg ctttcgcgcc acgcgctttc tcacgcgcgt tatccagcgc aacgtgataa
     5281 tcaaggatca gcggacatgc ttcagacagc agcagacgct cacgaacggg gatgccacgg
     5341 tcttccagtt ctttcatctc tttcatcagc gcggccggag acagcacaac accgttaccg
     5401 atgatgctgg ttacattctc gcggagaata cctgatggaa taagatggag aacggttttt
     5461 tcaccgttga ttacgagagt atggcctgcg ttgtgaccgc cctggtagcg tacaacatat
     5521 ttagcccgtt cagtcagaag atcgacgatc ttacctttac cttcgtcacc ccattgggtg
     5581 cccagtacga cgacgttgtt acccattttt caaaatcacc gtttgcttaa aaatggattc
     5641 taccatcgct ttttcagagt tacagcactt tttgcactca aaaatgacct cgatcagtct
     5701 gttttttgtt cagccaatcg ttttcctcaa catgtagtag accacaacgc ccgcaaccac
     5761 aagtccaccg ccaaaacgac gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat
     5821 cttcttccat gccttcgggt aaagcatcgg ccctaaacct tccagtacca aaaccagggc
     5881 aagcgccagc cagattgtcg aattcattca ttatccttat agaaaaagaa aaccaccgac
     5941 atcccagagg atgcggtggc tttattgacc tgtaccgcag tcgttatatt aacgcgttgc
     6001 ggaagtcggc gtcttcatgt agcggaagaa atcgctatcc gggctcatga ccatcacgtc
     6061 ctgattgcca gagaagctgt tctcataagc acgcaggcta cggatgaatg cgtagaagtc
     6121 cggatcttta ctgaatgcat cagcaaacag tttggctgct tcggcatcgc cttcaccacg
     6181 catgatacgg ccctgacgct cagcttctgc cagcgttctg gtcacttcat agtcggcagt
     6241 cgcgcgcagt ttttccgctt cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc
     6301 acgctcggcg cgcatacggt tgtagatcgc ttcagacact tcggtcggca ggttgatctg
     6361 cttgatacgc acatcgacaa cttcaatacc cagcgccgcc atactgttcg ggttgatgac
     6421 cggaactttg cccttcgtct cagccgttac gcgctctgcc gcttcggcaa tggcgttatc
     6481 tgccgccggg gtagtaactt catcttctgt acccgcagaa ccggagttca gcgcgtcacg
     6541 tacttcgagg gtcagacgac cacgggaatc agtgacgata tctttcacgt ccaggcgacc
     6601 aatttcagaa cgcagacggt cagagaactt acgtttcaac agcacttccg cttgcgaaat
     6661 gtcgccacca cccgttgcca ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta
     6721 agagtcgacg atcaggtctt tcttctcttt ggtcacaaag cggtcggcct ggttgtccat
     6781 ggtctgaata cgtgcgtcga gcattttcac cgtttcaatg aacggtatct tgaaatgcag
     6841 acccggctca taaaccagag gtttgttgtc atcgtcacgc agtaccttac caaaacgcag
     6901 cgtaataccg cgctcacctt ctttgacgac aaagacagac atgtaaagca ctaccagcac
     6961 gatgatgata atcgcgataa ctgacttacg catcgttatt ccccctgacg ctggtagtcg
     7021 ttacgctgcg cattggcgcg gcgttggtcc ataatatcgc cctgactggt ggacgacgtg
     7081 ttgcttgctc cactggttgt ggaggaagag gctggcggca gacgcagcag attgctggca
     7141 ccgttatcgc tcttcgccgc aggggcgtta ccacctttca gcatctggtc taacggcaga
     7201 accatcaggt tgccaccttt gtcgttaacc agcactttgc gggtgttgcc caacactttt
     7261 tccatcgtct cgatatacag acgctcgcga gtaatttccg gcgcggcttt atattccggc
     7321 agaagtttag caaagcgcgc cacttcaccc tgagcttcca ggatggtctg ggccttgtac
     7381 gcacgcgcct cttcgaggat acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg
     7441 gtatacgctt ctgcttcacg aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg
     7501 tcaaacgccg cttttacttc ttccggcgga cgagctgcct ggaagttgac gtccagcagc
     7561 gtgataccca tgtcatacgg gcggatagtc tcttccagtt cgcgctgagt atcgctacga
     7621 atcacggtac gaccttccgt cagaatgcgg tccatggtgt atttaccgat aactccacgc
     7681 agggcgctgt cggtagcctg acgcaggctg tcatccgggc tggtcacgct atacagatat
     7741 ttttccggat tggtgacgcg atactgcacg ttcatctcaa cgcgcactac gttctcgtcc
     7801 gacgtcagca tcacaccaga agcggccagt tcacgcacgg cttccacgtt caccggtttg
     7861 acttcgtcga taaacgtcgg tttccagttc agacccggct caaccagatg gctgaattta
     7921 ccaaagcgtg ttaccacgcc gcgttcggct tctttaatgg tatagaaacc actggccgcc
     7981 cagataatga caatcgctgc cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc
     8041 gggccttgcg atgaactgcc accgccagat ccggtgcctt taccgccgcc cagaccaccg
     8101 agctttttgc tcagtttgcg gaagatatca tctaaatcag gtggcccttg atcgcgaccg
     8161 cctttgtttc catttccctc agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg
     8221 tcttgtccgt tattaccggg ctgattccac gccatgtttg tgctccatat ttgttatgcg
     8281 gtgatccctg ttgtctttcg acgccagagg atatgactcc acgcttcaga cgctacgccg
     8341 ttagatcagg taatcgatca acgccggttc ttgtttacag aggcgacgcc agtcaacgat
     8401 cggcatacga acttgcagac ttacgctgcc gtcctcctcc atccactctt tttctattgc
     8461 ctgaagctga taaaaacgac ttctcagacg cccttcctgc ggtggcagac gcaatgtatg
     8521 ctgcgccacc tcgccggaaa gccgctccgt caaagcctga aaaagctgtg gtatccccgc
     8581 tccggtctgt gcggaaagcc agacacggat cggtttgttc tcttcgtccc gatcaatacg
     8641 cggttcgaaa tcttccagca tatcgatctt gttcatcacc agcagggttg ggatctcgtg
     8701 agcgtcgatc tcttcaagaa ccgtattcac cgcttcgatg ttttcttgta cacgcacatc
     8761 cgccgcatca atgacgtgca gcagtaatgt ggcttgccgc gtctcttgta acgtggcttt
     8821 aaatgctgcc accagatcgt gcggcaggtg gcgaataaac cctacggtat ctgcaagtac
     8881 ggtttcaccg acatctgcaa cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa
     8941 ctggtctgcc gcgtagaccc gcgcttcggt gatgcgattg aaaagggtag atttaccggc
     9001 gttggtatat cccaccagcg aaacagtagg aacgtcggct ttgatacgcg attgccgccc
     9061 ctgctcacgc tgcttttcaa ctctttccag gcgcgactgt atctgcacga tgcgattacg
     9121 caacaaacga cggtcggttt cgagctgggt ttcacccgga ccacgcaaac caatcccgcc
     9181 tttctgtctt tcaagatggg tccagccacg caccaggcgt gtagccagat ggcgcagctg
     9241 cgccagctca acctgcaact taccctcatg ggtacgcgca cgttgggcga aaatatctaa
     9301 aataaggccg gtgcggtcga taacacgaca ctcgcacaaa cgctccaggt tacgctcttg
     9361 cgccgggctc agggcatggt caaaaagtac gaccgaagca cccgtcgctt tgacagcttc
     9421 cgcaatttca actgctttac cttcacctac aaaatacttt gggtgcggcg ctttacggct
     9481 accggtaatc acctgcaatg cttcgacacc ggcggaagag accagagatt caaactcctg
     9541 gaggtcttcc atatctttgt cttgcgtaaa atagatgtgt accagtaccg cctgctcacc
     9601 agcatcataa cggtcaaaca agcgtataac cctctaaata gatcagcggg gaacgcagga
     9661 tcgctggctc cccgtgtaaa aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct
     9721 gttgcgcgga agtattctgc gcgctgctac catgatggta gttactgctg gtaccgccac
     9781 cggcgttgtt actgtgatga gaaaccgggc gagacgggac aacagtagaa atcgcgtgct
     9841 tgtaaaccat ctggctgacc gtgtttttca acaggatcac gaactgatca aaagactcga
     9901 tttgcccttg cagcttaata ccattcacca aataaataga aactggaaca cgttcccgac
     9961 gcagtgcgtt caggaacgga tcttgtaaag attgcccctt agccattctc tcttttcctt
    10021 atatgcttat ttgtactttg aacctttcga ttctgaaaaa attgcgcacg atacgtctca
    10081 attgtacaca ttcagcctgc gatagcacca acaacctgta atacttcgtc acgcgcctgt
    10141 tctggttttt cactatcaag ccagtgaacc ccttcccaac cacgcagcca ggttatctgc
    10201 cgcttcgcca actgtctcgt ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt
    10261 tcgccttcaa ggtaagacca catctggcga taacccacgc aacgaatgga aggcaagtcc
    10321 gtatgcaaat ctcctcgggc aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac
    10381 atctgatgaa aacgctgctc aattcgttga tggagcagtt cacggctcgc cggggcgatg
    10441 gcgaactgat gcacctgata cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa
    10501 gttttacccg aaatgaaaaa aacttccagt gcccgggaaa gcctttgtgg atcatttgga
    10561 tgaatccttg ctgccgcaac cggatcgatc tcctgaagtt gacgatgcaa tgactcccaa
    10621 ccttgctctg ccgcctgttg ctcaattctg gcccgtactt ccgggtctgc cgacggtagc
    10681 ggcgacaacc cttccagcaa tgccttgaaa tacaacatcg taccgcccac taacagtggg
    10741 atccgccccg ccgcggtgat atcggccatt tccgccagcg catcgcggcg aaaatcagca
    10801 gccgagtaag cctgcgacgg atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac
    10861 tcttcagcgt tcggcttcgc cgtcccgata tccatccctt tgtaaataag ggcagaatca
    10921 acgcttatca actctactgg taaaatttta cgcagttcaa tggctaacgc cgttttaccg
    10981 gaggccgtcg gccccatcaa aaaaatcgcc ttaggcaggc tcgccttact gatatcactc
    11041 atctttcagg gcttttatcg ccggatgtaa atcaacagat tgtaacagac cacccggcgg
    11101 cgttttcaca agttgcggac ataaccgttc cacgtccgcc agcagggtta tggcctgtgc
    11161 cattgaccac tgcgcatgtt cgctcatcag atttcgtgca atccactgcg caatattgcc
    11221 aggttcgaat acggactgct tcgccaggta gcctatcagt tcaggaatca agatttgtaa
    11281 attttgttgg cgtaagggta aaggcacggc cctgatggtc acatgctgcg catcggactg
    11341 aaattcaata cccaattccg ccagggcaga ctgcgctttt tctaatgccg atttttcttc
    11401 gccagaaact tttagccgca acggaatcag caacggctgg gcgcaaacgg gcgcttcacc
    11461 cggcgtcaat tgtgcctgac gcagccaacg ttctgccact ggcaaggcta aaagtgaaat
    11521 gttgccgtcg cgctccagca acgcacagtc ggaatggacg atagtcagta cccgaccaaa
    11581 actctgactg ttcgccgcaa gtgcaggttc ctgcggttcc ggcgcttttg gtttttgcat
    11641 cggcgcgggc gtttgcaaaa gctggcgata cacttcacct tgctgtttct ggtaacctgg
    11701 ctgcgcattc ggccaggggg cagccggacg actgcctgag gcgggcgcag gagagtagcg
    11761 cggagctacc ggctcacgag ctgccggttc tgcaaagtga ttgcgccccg ccgccacgcg
    11821 gttttccgga atgggacgcg gtgcaggttg gggttcatcg tccagcggta gcggcgtttc
    11881 cagttgctgt tgtagcacgc tcagcacgcc ctgatagata aaatcatgca ccagacgcga
    11941 ctgatggaaa cgcacttcgt gtttggcggg gtgtacgttg acgtccacct gatgcgggtc
    12001 gatctccaga tacaacacaa acgccggttg ttgatcggcc cccagtttgt cttcgcaggc
    12061 ctggcggatc gcgtgattga tcaggcgatc gcgcatcatg cgaccattca cgtagcaata
    12121 ctgaatttcc gccagtgcgg gcgtggtgtg atttggatcg gccacccagc cgcgcagggt
    12181 gagatcgccg tgttgccatt caatcgccag cgcttgttcg agaaaagcgg tgccacaaat
    12241 cgcgcctaag cgccgttctt tttgcccgcc ttccggcact gcgcggtact gacgcacaat
    12301 tttaccgtta tgcgacaggt tgatcgtgac gtcgaaacgc gccagcgcaa tgcggcggat
    12361 gatttcatcg atatggttaa attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg
    12421 ggtgttgtag aacagatcca gcacctccag cgtcgttccc accggatgcg ccgccggttt
    12481 aaccgtcacg tccatatcgc gcccttcggc ataggcctgc caggcttcct gctgttctgc
    12541 ggtgcgtgaa gtgagcgtca ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg
    12601 aaagcccagg ctgataatgg cttcgagatc gtccagagag gcgattttac tggtggcatg
    12661 acgagccagc gccagcgcca gctcatcttt tttgataccg cagccgttat cacgaatgcg
    12721 gataagtttc gccccaccgc gttcgatatc aatatcgata cgcgtcgcac ctgcatcgag
    12781 gctgttttcc accagttctt tgactaccga cgcaggtcgc tcgaccacct cacctgcggc
    12841 aatctggttc gccagttgtg gcggtaagac ctgaattggc atcaatcgtc cttagtttgg
    12901 cagcgaacga tctggcgtcg tcaccgtact ggcagtttgt gccgttgccc cctgcggcgc
    12961 agattgcatc ggatgcgcaa ggaaataatt gcgcaggcct ttataaatgg cttctgccag
    13021 ctgttgttgg taatcgtcgc tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc
    13081 ggtttcgacc agtactgatg ggatatccgg cgaacgcaga acgccaaggc tggcatgttc
    13141 tggtcgacgt ttatgaattt cgccaatgcg ttgcaactga ctgatcatac tggtcgctac
    13201 atcataccct acccgctggg aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata
    13261 ggggtcagac tgactgttcg ccagcacatc gcccgcgccg cccagcagct ccgactgttt
    13321 ctcatgctgt tccagccagc ttgccatctc gctgtttgca cgacggtttg agagcaccca
    13381 tacggaagcg ccagtcgcac tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa
    13441 attggcgttt tgcttacgtg ccacatcgct gcgccccatc accgagataa agtaatcccc
    13501 gtcacgggtt aaaacgcctt taaacatcgg atcgtcattg agcaaggtac gtaatttacg
    13561 cgcgatggcg atggtgacat ttttctcccg cgtaccaccg gggccgatag cgccagggtc
    13621 ctgaccgccg tgtccggcat caatagcgat gataatttta tcgccagtgt tagccgtcgc
    13681 gcgggctgcc ggacgcgtta ccgtattact gctgataaca cccgtagtgc ggttactttc
    13741 cgttttaaac ggattgcgcg ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc
    13801 aacgcgtttc gcaaccacgg gcggcggagg aggcggtggc ggcgcatcgg cgttaatcgt
    13861 aaagacgaca gtgtaattgc tgccattctg ccgcttcacc gcttcggttt taccattttc
    13921 ggtaagatcg accaccagcc gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc
    13981 cttcaccaga ttattgccgc tgaacaacaa tggcagtccc tgaatcacgc ccgtttgttt
    14041 gatatcgagc gccacggtgc gtttgctttg atggctaaac gcataatcag gatcgccaat
    14101 aaaactcaac gttatccgcg cctgttggtt gccgttagaa acctgaatat cagagagcgt
    14161 cgcggcaccc acctgcgcgc acagcagcag caacgtcgct accaaccaat ttctgatgcg
    14221 atacatcatc ccgccacctt tcaaaggtta accggctaaa cgcgccagca acaattcacc
    14281 cgcagaggaa accgcactca cgcgcgcctc acggccttgt gcctgataat caatgtgtat
    14341 ttcgacatcc gggtcaggaa gaacacctgt accttgttgt ggccactcca ccaggcagat
    14401 ggcatcgttg gcaaaataat cgcggatccc cataaactcc agctcctcgg gatcggcaag
    14461 gcggtacaaa tcaaagtgat aaaccattaa gttgtcgagc gtatagggtt cgaccagcgt
    14521 ataagtgggg cttttgacat taccctgatg gcccaaagcc tgtaaaaagc cccggctaaa
    14581 ggtggtttta cctgcgccta aatcgccata cagatagatt acggttgcgc catcgcaggc
    14641 tttcgctacc cgctcgccca ggtctaatgt tgcctgctca tcagggagcg gaattactcg
    14701 attcatcatg gtttttatca gtcacttccg ggttaacaat acgctgtagc gtggaaaaga
    14761 gatcggttgc cagcatcccg cgcgttccaa aacgcgccgc cagtacgtca gctgcctcac
    14821 cgtgcgcgac acagcccgca caggcggcat catacggcga tagtttttgc ccaagcaatg
    14881 cgccaataat accagagagc acatcgccca tgccgccgct cgccatgcct gcatttccgg
    14941 catcaataat gcctaaagcg tcaggatggg cggcgaccac ggttccggca cctttcagca
    15001 ccgctacgcc gccataacgt tgtaccagac gttgggcgca atgtaagcgg tcactttcaa
    15061 tttcagcgac ggaacagcct aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc
    15121 gattgtgacg cttatcggga ttgattgcca gcaggttcaa tgcatcggca tcccacaaca
    15181 tcggtttgcg aaaattctca actttttgca gggctttttt cccccactct tgctggccca
    15241 gaccgggacc aatcaccacc acatcggccc attccaggct ttcggtaaga gagtccatag
    15301 tcagttcatg caccatcaat tctggtcgtg cagtcagcag cggcgcaatg ttttcactgc
    15361 gggtcagaac tcggactaaa ccagcaccag cacgcagcgc cgcttccccc gtcatacgaa
    15421 tagcccccgc cgtgccgtga tcgccaccga taatcaccag ccgcccgtga tcgcctttat
    15481 gcgaagtcgg gcgacgcggt ttcagccagt gagaaagttg ttctgccgaa aaccgctgaa
    15541 ttttcgtctc ctgacctgcc agccagctat ccagccccag tgagtcaaaa tgcagttgtc
    15601 ctgtaacatc ccgcgctttt ccagtgagca agcctggttt cagcgcaata aaagtgatgg
    15661 tgtgatcggc gttgatcact gcgcctggcg tagcgccggt ttcagccagc aggccggaag
    15721 ggatatcaac cgccacaatc ggcgcagaat gggaattagc gtggtcgatt aactggctaa
    15781 tggattcgcg gggcgcttgc tgcaagccgg taccgagcag cgcatcaacg atcagatcta
    15841 ccgattcggg ccagacaata ttcgaagcat ggatctcgcc acccgcgttt aaccatgctt
    15901 cgcgtgccag cgcggcctct tccggcaacg gtttgtcgct atcctgggcc aacaacgtga
    15961 cctcaatgcc gagcgctttg gccagtcgcg cgaccacgta gccatcgccg ccgttattac
    16021 catgaccgca cagcaccagc cagtggcggg cgtcaggata cgccgaacga cacacctgga
    16081 atgcggcctc gccagcgcga agcatcagct catagagtgt gagccccagc acatctgccg
    16141 cctcgcgttc tccgcggcgg atatcgtcgg cgtaccagac ggtgtgtggt atacttacgg
    16201 ggtttttctt cattgtatgg tccgtcatgt cagagcccct cgatctcaat cagttagcgc
    16261 aaaaaattaa acagtggggg ctggaactgg gctttcagca ggtaggtatt accgataccg
    16321 atctcagcga gtccgagccc aaactgcaag catggctgga caaacaatac cacggcgaaa
    16381 tggactggat ggcacgtcac ggtatgttgc gcgcccgccc tcacgaatta ttgcccggta
    16441 cgctgcgcgt gatcagcgtg cggatgaatt accttcctgc taacgccgca tttgccagca
    16501 cgctgaaaaa ccccaaactc ggctatgtta gccgttatgc gctgggccgt gactatcaca
    16561 aacttctgcg caaccgactc aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc
    16621 tgaattttag accgtttgtc gattctgcgc ctattctcga gcgcccgtta gctgaaaaag
    16681 ctgggctcgg ctggacaggt aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct
    16741 tctttttagg cgaattgctg gtcgatattc ctctgcccgt ggatcaacca gtcgaggaag
    16801 gatgtggtaa atgcgtggcc tgtatgacga tttgcccgac cggtgccatc gtcgagccat
    16861 ataccgtcga tgctcgccgc tgtatctctt atctcaccat cgaactggaa ggggcgatcc
    16921 cggaagagtt gcgaccgtta atgggaaacc gtatttacgg ttgcgatgac tgccagctta
    16981 tctgcccgtg gaatcgctat tcacaactca ctacagaaga cgatttcagc ccgcgtaagc
    17041 cgctacacgc accggaactc attgagttat tcgcctggag cgaagagaag tttttaaaag
    17101 tcacggaagg ttcggcgatt cgccgtatag gtcacctgcg ttggctgcgt aatattgctg
    17161 tagccttagg caatgcgccc tgggatgaaa cgattctggc ggcgctggaa agtcgtaaag
    17221 gtgagcaccc acttcttgat gagcacatag cgtgggcgat tgcgcagcaa atcgagagac
    17281 gaaatgcgtg catagtcgaa gtgcaattgc cgaaaaaaca gcgtctggtt cgggtgattg
    17341 aaaaagggtt accgcgtgac gcctgattca tccacagctt gtgaataaaa ataaaaacgc
    17401 attgcaattc aagacgaata aaatcgtcaa gtgatcgact cgacaaaa
//
LOCUS       DAZUCE010000098        17433 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZUCE010000098 DAZUCE010000000
VERSION     DAZUCE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.098"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1336)
                     /gene="djlB"
                     /locus_tag="ACHY03_002733"
     CDS             complement(<1..1336)
                     /gene="djlB"
                     /locus_tag="ACHY03_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFS3905942.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFSALLASDSERFLPSAWQRFIQQLNY
                     CSMDEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENTGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HGNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDD"
     gene            complement(1333..2040)
                     /gene="ybeR"
                     /locus_tag="ACHY03_002734"
     CDS             complement(1333..2040)
                     /gene="ybeR"
                     /locus_tag="ACHY03_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFS3905943.1"
                     /translation="MDMESKKILFALSTPMEIRNECCLPSHSSPKMYLGTRFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            2204..3181
                     /gene="ybeQ"
                     /locus_tag="ACHY03_002735"
     CDS             2204..3181
                     /gene="ybeQ"
                     /locus_tag="ACHY03_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFS3905944.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEQGDSEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVAWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3251..3733)
                     /gene="ybeL"
                     /locus_tag="ACHY03_002736"
     CDS             complement(3251..3733)
                     /gene="ybeL"
                     /locus_tag="ACHY03_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFS3905945.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            3969..6551
                     /gene="leuS"
                     /locus_tag="ACHY03_002737"
     CDS             3969..6551
                     /gene="leuS"
                     /locus_tag="ACHY03_002737"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFS3905946.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAISDKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6566..7147
                     /gene="lptE"
                     /locus_tag="ACHY03_002738"
     CDS             6566..7147
                     /gene="lptE"
                     /locus_tag="ACHY03_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFS3905947.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7147..8178
                     /gene="holA"
                     /locus_tag="ACHY03_002739"
     CDS             7147..8178
                     /gene="holA"
                     /locus_tag="ACHY03_002739"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFS3905948.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANCGVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8180..8821
                     /gene="nadD"
                     /locus_tag="ACHY03_002740"
     CDS             8180..8821
                     /gene="nadD"
                     /locus_tag="ACHY03_002740"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFS3905949.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            8845..9456
                     /gene="cobC"
                     /locus_tag="ACHY03_002741"
     CDS             8845..9456
                     /gene="cobC"
                     /locus_tag="ACHY03_002741"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFS3905950.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKSATLRVLNSRAIGVENA"
     gene            9716..10033
                     /gene="rsfS"
                     /locus_tag="ACHY03_002742"
     CDS             9716..10033
                     /gene="rsfS"
                     /locus_tag="ACHY03_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFS3905951.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10037..10504
                     /gene="rlmH"
                     /locus_tag="ACHY03_002743"
     CDS             10037..10504
                     /gene="rlmH"
                     /locus_tag="ACHY03_002743"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFS3905952.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10535..12436
                     /gene="mrdA"
                     /locus_tag="ACHY03_002744"
     CDS             10535..12436
                     /gene="mrdA"
                     /locus_tag="ACHY03_002744"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFS3905953.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12439..13551
                     /gene="mrdB"
                     /locus_tag="ACHY03_002745"
                     /gene_synonym="rodA"
     CDS             12439..13551
                     /gene="mrdB"
                     /locus_tag="ACHY03_002745"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFS3905954.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13562..14650
                     /gene="rlpA"
                     /locus_tag="ACHY03_002746"
     CDS             13562..14650
                     /gene="rlpA"
                     /locus_tag="ACHY03_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFS3905955.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQATSQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14789..16000
                     /gene="dacA"
                     /locus_tag="ACHY03_002747"
     CDS             14789..16000
                     /gene="dacA"
                     /locus_tag="ACHY03_002747"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFS3905956.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16111..16374
                     /gene="ybeD"
                     /locus_tag="ACHY03_002748"
     CDS             16111..16374
                     /gene="ybeD"
                     /locus_tag="ACHY03_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFS3905957.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16475..17116
                     /gene="lipB"
                     /locus_tag="ACHY03_002749"
     CDS             16475..17116
                     /gene="lipB"
                     /locus_tag="ACHY03_002749"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFS3905958.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4385 a         4377 c         4453 g         4218 t
ORIGIN      
        1 catcgtcaac gggcattggc tgcggttgcg gctccggttc aacagtttgc ttctcggtaa
       61 aattcgccgc ctgaattaat aatcgacgaa taattaatgc ttccagtgga caacgatttt
      121 ctgcatcaag acggcttacg atcggcatta atgattcttc tgttggttgg ctggcctgca
      181 ctaattgttt ggcaactaaa tataatggcc ataagggatg ttctgtatca cgcgtgagtg
      241 agatccgcgg cttacgtcct ttgtgcaaaa ccatctcgac cagtgaacgc cccagagcag
      301 agagtttttc atcgctgagt atttcgtata aatgaaaacg cgtacgggcg ctgtcgccgg
      361 ggatataaag tagcggtttt ccctgatcgt taaccagttc acgagcttca atcatcatca
      421 ccactaacgg gtaataatcg acccgatgct gacggcacca ttgcaatatt aaagcatccg
      481 cctgggtgga gggatactcg cgccagtagt cacaaagttc tgccagcaag ctttcgcctt
      541 ctccacaata gacgcgttgt gcgtattcat aataacgcgc atcttcatta tcagattcag
      601 cttcccgcca cgaccgggcg taatccagga gttcgggaat tcccaggcga cctgcgctat
      661 accagcggag taaatttccg tgcagttttg catcatcggg gataatcact gcgcgatgtt
      721 gcgctaaata caatgtcagc cattctggat gcgatgacca aatccgagcg aacatttggt
      781 aaaaatcgat ggtgtcattt tgcacggcaa cgggcagatg cagaatggtc tggaagttaa
      841 aaacattccc cttcgcaatg gcatataaaa aggattccag ttcttcttcg tctatttccc
      901 cggtattttc ctcctgtaac caccgcaatc tttctgctaa taacaccacg cactcgaagg
      961 ataaatgggc agtgttcatg gctattgtgc acagcgacca gcgtaattca tcaatctcat
     1021 ccatcgagca ataatttaat tgctgaatga atcgctgcca ggcggagggc agaaaacgtt
     1081 cactatcaga ggcaagtaac gcactaaagg cgagcagaat ttcatgttct gctacctcat
     1141 attcttctgg ttgccaaaca gatttagcag gcgactgcgc aatccggagc gcttcctcat
     1201 acgcctgacg aagttgttta aaaccctgcg gatcggtttc tggatgaaag gacggtaaca
     1261 gcgccagata agcgcggcgg ataatatcga cgtcagtcgt ttcctctatg ttgaggatct
     1321 tccagcaatt tttcataggt cgctcatatc cttaagcgtt tccgggcatg tggggtagtc
     1381 agccagtggt tgccagggta aggtcgcgta atatgcatcg tcactggcaa ataattgata
     1441 aaacatatca ttgccggaat cgtcgtattc tttacgcgct aagaattcac gtaataaagg
     1501 cagattatca tcttcgggag actgccaata ttgccgacca aaggtataac cggcaaagta
     1561 ttgccgccag ttactataat aacgtagagc ccgtagatga atacgcgact ggatccatag
     1621 actttcttct tcactcaacc agttattaag tacgcccatt cgacttaaaa aacccatccg
     1681 gacataatcc catgctttaa ttcctccctc accacagcat tcggctgtgc tggcgacaaa
     1741 ttgcgcataa gcctgacctt gctcatttaa ttcagcaaga tagtcacgcc attcacatgg
     1801 agaataacga aaccagcggt gataaaaccc tgccaaccgc gcggcatgac cgttatcgat
     1861 cattcggtga attgtgcgca gcaggtcatc acggtcatca attccccagg aggaagaaag
     1921 gtcaaaaaag cgtgtaccta aatacatttt gggtgatgaa tgcgaaggta aacagcattc
     1981 attgcgtatt tccatcggag tggaaagtgc aaacaatatt tttttcgact ccatgtccat
     2041 ataatttatc cttaaacatt catttaatga atgcaaacta cattacgagg ctcatttatt
     2101 tatacttttc tgacacaaca taatacgcat aaaataagac ttacgcaaaa acggaacaca
     2161 taaaaatata atatgtttta tctgcaacca taaggaaatg ataatgattt ttacgtcaag
     2221 ttgctgcgat aatttgtcaa tagatgagat tatcgaacgt gctgaacaag gcgatagtga
     2281 ggctcaatat attgttgggt tttattataa tcgcgatagc gcaattgact ctccggacga
     2341 cgaaaaggcc ttttactggc tgaagctggc cgctgagcaa ggtcattgtg aagcacagta
     2401 ttccttaggg caaaagtata ccgaggataa aagccgtcat aaagataatg agcaagccat
     2461 cttctggctg aaaaaagctg ccctacaagg ccatactttc gcttccaacg cccttggctg
     2521 gatactggat cgtggagaag accccaacta taaagaagcg gttgcctggt atcagatagc
     2581 cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg tggatgtaca gaaatggcaa
     2641 cggagtcgca caagactata cgctggcatt tttctggtac aaacaagctg cattacaagg
     2701 ccatagtgac gcgcaaaaca atctggccga tctttatgaa gacggaaaag gcgttgctca
     2761 aaacgagaca ctcgccgcat tctggtattt gaaaagcgca cagcagggta atcggcatgc
     2821 tcagtttcaa attgcatggg attataacgc tggcgaaggg gtggaccagg actataagca
     2881 agcgatgtac tggtatctga aggctgccgc tcaggggagc gtcggcgctt acgtcaacat
     2941 cggttatatg tataaacacg gacaaggcgt tgagaaagat tatcaggctg cctttgaatg
     3001 gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg tataacctgg ccattatgta
     3061 tcactacgga gaaggaagac ctgtcgatct ccgacaggct ctcgacctgt atcgtaaagt
     3121 tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt gagactgaag atttactgta
     3181 gagataaaaa caagggccgg ggaatttcat tcggcccata ttctctttat tgctgtgcga
     3241 aatcagacta ttacggctca aacgggcgtc tctggaactg gtcatgacca catttcgggc
     3301 atagcgtcag cacttccggt gtgtagatcg ggagatggaa gtgacatttc tcgcagacca
     3361 gatttcccag cccgaccact tctccgctgt gataaacccc atgatgattg aggtcctgga
     3421 aaacttcgcg ccattcaagc tgcgttttat cggtgatgtc tgccagctcc tgccacaagc
     3481 tttctttaat cacccgcata aagacgctgt cagattcttc tttcaggctc tcttcatagc
     3541 tcatggcgaa ctcttccagg tcacgtctga cagctcgcgt cagctcatcg acctcggttc
     3601 gcgttaactc ccctgttttt attacgcgct cgcgcgcctg ttccaccagt gcgtcgatat
     3661 cacgttcgcc attgcgcagg cgttcgctca gtgacgcaac cagttcacgg taatattgag
     3721 caaccttgtt catcgtttca tctcccgggt cgttaactga ctataataat agacgttctt
     3781 tgttccttgc tttgctaata cgcccacaaa ctctgtaaat taatgtatga ataatccggg
     3841 catcatttca acgtcgagtg cgcgaaaggc tgttttgacg cggacgtttg ggctatgcta
     3901 tgcggatctg aaaaaccacc tctaacgcta cttttgtagc cgtaatgaaa acaggaccac
     3961 tggctgccat gcaagagcaa taccgcccgg aagagataga atccaaagta cagcttcatt
     4021 gggatgagaa gcgcacattt gaagtaaccg aagacgagag caaagagaag tattactgcc
     4081 tgtctatgct tccctatcct tctggtcgac tacacatggg ccacgtacgt aactacacca
     4141 tcggtgacgt gatcgcccgc taccagcgta tgctgggcaa aaacgtcctg cagccgatcg
     4201 gctgggacgc ctttggtctg cctgcggaag gcgcggcggt gaaaaacaac accgctccgg
     4261 caccgtggac gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg ctgggctttg
     4321 gttatgactg gagccgcgag ctggcaacct gtacgccgga atactaccgt tgggaacaga
     4381 aattcttcac cgagctgtat aaaaaaggcc tggtatataa gaagacttct gcggtcaact
     4441 ggtgcccgaa cgaccagacc gtactggcga acgaacaagt tatcgacggc tgctgctggc
     4501 gctgcgatac caaagttgaa cgtaaagaga tcccgcagtg gtttatcaaa atcactgctt
     4561 acgctgacga gctgctcaac gatctggata aactggatca ctggccagac accgttaaaa
     4621 ccatgcagcg taactggatc ggtcgttccg aaggcgtgga gatcaccttc aacgttaacg
     4681 actatgacaa cacgctgacc gtttacacta cccgcccgga cacctttatg ggttgtacct
     4741 acctggcggt agctgcgggt catccgctgg cgcagaaagc ggcggaaaat aatcctgaac
     4801 tggcggcctt tattgacgaa tgccgtaaca ccaaagttgc cgaagctgaa atggcgacga
     4861 tggagaaaaa aggcgtcgat actggcttta aagctgttca cccattaacg ggcgaagaaa
     4921 ttcccgtttg ggcagcaaac ttcgtattga tggagtacgg cacgggcgca gttatggcgg
     4981 ttccgggtca cgaccagcgc gactacgagt ttgcctctaa atacggcctg aacatcaaac
     5041 cggttatcct ggcggctgac ggctctgagc cagatctttc tcagcaagcc ctgactgaaa
     5101 aaggcgtgct gttcaactct ggcgagttca acggtcttga ccatgaagcg gccttcaacg
     5161 ccatctccga taaactgact gcgatgggcg ttggcgagcg taaagtgaac taccgcctgc
     5221 gcgactgggg tgtttctcgt cagcgttact ggggcgcgcc gattccgatg gtgacgctgg
     5281 aagacggtac cgtaatgccg accccggacg accagctgcc ggtgatcctg ccggaagatg
     5341 tggtgatgga cggcattacc agcccgatta aagcagatcc ggagtgggcg aaaactaccg
     5401 ttaacggtat gccagcgctg cgtgaaaccg acactttcga cacctttatg gagtcctcct
     5461 ggtactatgc gcgctacact tgcccagagt acaaagaagg tatgctggat tccgaagcgg
     5521 ctaactactg gctgccggtg gatatctaca ttggtggtat tgaacacgcc attatgcacc
     5581 tgctctactt ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg aactctgacg
     5641 aaccagcgaa acagttgctg tgtcagggta tggtgctggc agatgccttc tactatgttg
     5701 gcgaaaacgg cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa cgtgacgaga
     5761 aaggccgtat cgtgaaagcg aaagatgcgg caggccatga actggtttat accggcatga
     5821 gcaaaatgtc caagtcgaag aacaacggta tcgacccgca ggtgatggtt gaacgttacg
     5881 gcgcggacac cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg actctcgaat
     5941 ggcaggaatc cggcgtggaa ggggctaacc gcttcctgaa acgtgtctgg aaactggtgt
     6001 acgagcacac agcaaaaggt gatgttgcgg cactgaacgt tgatgcgctg actgaagatc
     6061 agaaagcgct gcgtcgcgat gtgcataaaa cgatcgctaa agtgaccgat gatatcggcc
     6121 gtcgtcagac cttcaacacc gcaattgcgg cgattatgga gctgatgaac aaactggcga
     6181 aagcaccaac cgatggcgag caggatcgcg ctctgatgca ggaagcgctg ctggccgttg
     6241 tccgtatgct taacccgttc accccgcaca tctgcttcac gctgtggcag gaactgaaag
     6301 gcgaaggcga tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg atggtggaag
     6361 actccacgct ggtcgtggtg caggttaacg gtaaagtccg tgccaaaatc accgttccgg
     6421 tggacgcaac ggaagaacag gttcgcgaac gtgctggcca ggaacatctg gtagcaaaat
     6481 atcttgatgg cgttactgta cgtaaagtga tttacgtacc aggtaaactc ctcaatctgg
     6541 tcgttggcta agcgcgggag gaagcgtgcg atatctggca acattgttgt tatctctggc
     6601 ggtgttaatc accgccgggt gtggctggca tctgcgtgat accacgcagg ttccttccac
     6661 tatgaaggtc atgatcctgg actcaggcga tccgaacggg ccattaagcc gtgcggtgcg
     6721 taaccagtta cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc gtaaggacgt
     6781 tccatccctg cgtttgggta aagtgagcat cgcgaaagat accgcatcgg tattccgtaa
     6841 cggtcaaaca gcagagtatc agatgatcat gacggtgaat gcgaccgtgt tgatccccgg
     6901 ccgtgatatc tacccgatta gcgccaaagt cttccgttcg ttctttgata acccgcaaat
     6961 ggcgttagcg aaagataacg aacaagacat gatcgtaaaa gagatgtacg accgtgctgc
     7021 cgaacagctg attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt ccgacgaaga
     7081 acagacgtcg accacaacgg atactccggc aacgcctgca cgcgtctcca ccacgctggg
     7141 taactgatga ttcggttgta cccggaacaa ctccgcgcgc agctcaatga agggctgcgc
     7201 gcggcgtatc ttttacttgg taacgatcct ctgttattgc aggaaagcca ggacgctgtt
     7261 cgtcaggtag ctgcagcaca aggattcgaa gaacaccaca ctttttccat tgatcccaac
     7321 actgactgga atgcgatctt ttcgttatgc caggccatga gtctgtttgc cagtcgacaa
     7381 acgctattgc tgttgttacc agaaaacgga ccgaatgcgg cgatcaatga acaacttctc
     7441 acactcaccg gacttctgca tgacgacctg ctgttgatcg tccgcggtaa taaattaagc
     7501 aaagcgcaag aaaatgccgc ctggtttact gctcttgcga attgcggcgt gcaggtgacc
     7561 tgtcagacac cggagcaggc tcagcttccc cgctgggttg ctgcgcgcgc aaaacagctc
     7621 aacttagaac tggatgacgc tgcaaatcag gtgctctgct actgttatga aggtaacctg
     7681 ctggcgctgg ctcaggcact ggagcgttta tcgctgctct ggccagacgg caaactgaca
     7741 ttaccgcgcg ttgaacaggc ggtgaatgat gccgcgcatt tcaccccttt tcattgggtt
     7801 gatgctttgt tgatgggaaa aagtaagcgc gcgttgcata ttcttcagca actgcgtctg
     7861 gaaggcagcg agccggttat tttgttgcgc acattacaac gtgaactgtt gttactggtt
     7921 aacctgaaac gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa gcatcgggta
     7981 tggcagaacc gccggggcat gatgggcgag gcgttaaatc gcttaagtca gccgcagtta
     8041 cgtcaggccg tgcaactcct gacacgaacg gaactcaccc tcaaacaaga ttatggtcag
     8101 tcagtgtggg cagagctgga ggggttatct cttctgttgt gccataaacc cctggcggac
     8161 gtatttatcg acggttgata tgaaatcttt acaggctctg tttggcggca cctttgatcc
     8221 ggtgcactat ggtcatctaa aacccgtgga aacgctggcg aatttgattg gtctgacgcg
     8281 agtcacaatc atccctaata atgttcctcc gcatcgtccc cagccggaag cgaacagcgt
     8341 acagcgtaaa cacatgcttg aactggcgat tgccgacaag ccattattta ctcttgatga
     8401 acgcgagcta aagcgcaatg ccccctctta cactgcgcaa acactgaaag agtggcggca
     8461 ggaacaaggg ccggacgtgc cgctggcgtt tattattggt caggattcat tgctgacctt
     8521 tccgacctgg tacgaatacg aaacgatact cgacaatgca catttgatcg tctgtcggcg
     8581 tccaggttac ccacttgaaa tggcgcaacc gcaataccag caatggctgg aagatcattt
     8641 gacacataac ccggaagatc ttcaccttca gcctgccggt aaaatttatc tggctgaaac
     8701 accatggttt aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaacg gtgaatcgtg
     8761 tgaggattta ttgccggaac cggtactgac ttacattaac caacaaggct tgtatcgctg
     8821 atacctgtcg ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg tgaaacgcaa
     8881 gcaaatgtcg atggtctgta cagcggtcat gctcccacac ccctgaccgc tcgcggtatt
     8941 gagcaagcgc aaaatctgca tacactgcta gatgatgttt cctttgacct ggttttatgc
     9001 agtgaactgg aacgggcaca gcataccgcg cgactggttc tcagtgaccg ccagctcccc
     9061 gttcatatca tccctgaact caacgaaatg ttttttggcg actgggagat gcgacatcat
     9121 cgcgacctca tgcaagaaga tgccgaaaac tatagcgcgt ggtgcaatga ctggcagcat
     9181 gctatcccca cgaacggtga aggatttcag gcattttcgc aacgtgtaga acgctttatc
     9241 gcaaggctta gtgaatatca gcactatcag aatattttaa tcgtcagcca tcagggcgta
     9301 ctgagtctgt taattgctcg tttaattggc atgcctgccg agtctatgtg gcattttcgc
     9361 gttgaccagg ggtgttggag cgccattgat atcaaccaaa aatccgcgac gctacgcgtc
     9421 ctcaatagcc gtgccatcgg ggtcgagaat gcatgacttt tctgtttttt tacgggtaag
     9481 ccgcaacgac cattgacagc cccgggcagg ctgatattct ccgcagccag actttttccg
     9541 ccagacacga ctttgtagaa attgttttac aaaaatggcg atgcaatctg cggcgcgggg
     9601 tgggatgata gcccactttc gaaagccgat tcggcgacaa ttgtcccgaa atcgcctctg
     9661 gttcaggtat actgacagac catttttatc tatttgattc acccaggggg aaaacttgca
     9721 gggtaaagca ctccaggatt ttgttatcga caaaattgat gacctcaaag gtcaggacat
     9781 catcgcctta gacgttcagg gcaaatccag catcaccgac tgcatgatca tctgtacggg
     9841 tacgtccagc cgtcatgtta tgtccattgc tgaccacgtt gtgcaggagt ctcgcgcagc
     9901 gggcctgtta ccgctcggcg tagaaggtga aaacagcgcc gactggattg tcgtggattt
     9961 gggcgatgtg attgtccatg tcatgcagga agagagccgt cgcctgtatg aactggaaaa
    10021 actctggagt taatgcttga agctgcaact tgtcgccgtg ggaacgaaaa tgccggactg
    10081 ggtacaaacc ggttttaccg agtacctgcg tcgttttccg aaagatatgc ccttcgagct
    10141 gattgaaatt ccggccggaa aacgcggcaa gaatgcggac atcaagcgca tactcgacaa
    10201 agagggtgag cagatgttgg cggccgcagg caaaaaccgc attgtcaccc tcgatattcc
    10261 aggcaagccc tgggatacgc cgcagttagc cgctgagctg gaacgctgga agctggatgg
    10321 tcgcgacgtc agtctactga ttggcgggcc tgaagggttg tcgcctgcct gtaaagcggc
    10381 ggcggagcag agctggtcgc tgtcggcgct taccctcccc catccgctgg ttcgcgtgct
    10441 ggtcgcagag agtctgtacc gggcgtggag catcaccacc aaccatcctt atcaccgtga
    10501 gtgataaggg agctttgagt agaaaacgca gcggatgaaa ctacagaact cttttcgcga
    10561 ctatacggca gagtccgcgc tgtttgtgcg ccgggcgctg gtcgcctttt tggggatttt
    10621 gctgctgacc ggcgtgctta tcgccaacct gtataatctg caaattgttc gctttaccga
    10681 ctaccagacc cgctctaatg aaaaccgcat taagctggtg cctatcgcgc ccagccgcgg
    10741 cattatctac gaccgtaacg gtatccctct ggccctcaac cgcactatct accagataga
    10801 aatgatgccg gagaaagtcg ataacgtgca gcaaacgctg gacgctttgc gcagcgtggt
    10861 agatctgacc gatgacgata ttgctgcatt ccgaaaagag cgcgcacgtt cacaccgttt
    10921 cacctctatt ccggtgaaaa ctaacctgac cgaagtacaa gtagctcgct ttgccgtcaa
    10981 tcagtaccgt tttccgggtg tcgaagttaa aggctataaa cgtcgttact atccttacgg
    11041 ttcggcgttg acccacgtca tcggctatgt gtcgaaaatc aacgataaag acgtcgaacg
    11101 cctgaataat gacggcaaac tggccaacta tgcggcaacg catgatatcg gtaagctggg
    11161 cattgagcgt tactatgaag acgtgctgca cggtcagacc ggttatgaag aggttgaagt
    11221 taacaaccgt gggcgtgtta tccgccagtt aaaagaagta ccaccgcaag ccggacacga
    11281 tatttacctg acgctggatc tcaaactcca gcaatatatt gaaacgctgc tggcgggtag
    11341 ccgcgcagct gtggtagtca ccgatccgcg tacaggtggg gtgctggcgc tggtttccac
    11401 gcctagttat gacccaaacc tgtttgttga cggtatctcc agcaaagatt attccgcctt
    11461 gttgaacgac ccgaatacac cgctggtgaa ccgcgccaca cagggggttt atcctcccgc
    11521 gtctacagtt aaaccctatg tagcggtttc ggcattgagc gccggggtga tcacgcgcaa
    11581 tacgacgctg tttgacccag gctggtggca actgccaggt tcggaaaaac gttatcgtga
    11641 ctggaaaaaa tggggccacg ggcgtctgaa tgtcacaaga tcgctggaag aatctgcgga
    11701 taccttcttc tatcaggtgg cctacgatat ggggatcgat cgcctctccg aatggatggg
    11761 taaattcggt tatggtcatt ataccggtat cgacctggcg gaagaacgtt ccggcaacat
    11821 gcctacccgc gaatggaaac agaaacgctt taaaaaaccg tggtatcagg gtgacaccat
    11881 tccggttggt atcggtcagg gttactggac agcgacccca atccagatga gtaaggcact
    11941 gatgatcctg attaatgacg gtatcgtgaa ggttccgcat ttgctgatga gcaccgccga
    12001 agacggcaaa caggtgccat gggtacagcc gcatgaaccg cccgtcggcg atattcattc
    12061 cggttactgg gagctggcga aagacggtat gtacggtgtt gctaaccgcc ctaacggtac
    12121 ggcgcataaa tactttgcca gcgcaccgta caaaattgcg gcgaaatccg gtaccgctca
    12181 ggtcttcggt ctgaaagcga acgaaaccta taatgcgcac aaaattgccg agcgtttacg
    12241 tgaccacaaa ctgatgaccg cctttgcgcc atacaacaat ccgcaagtgg ctgtcgccat
    12301 gattctggag aacggtggcg cgggtccggc ggttggtacg ctgatgcgcc agatcctcga
    12361 ccacattatg ctgggtgata acaacaccga tctgcctgcg gaaaatccag cggttgccgc
    12421 agcggaggac cattaatcat gacggataat ccgaataaaa aaacattctg ggataaagtc
    12481 catctcgatc ccacaatgct gctgatttta ctggcattgc tggtttacag cgccctggtt
    12541 atctggagcg ccagcggtca ggatattggc atgatggagc gtaaaatcgg ccaaatcgcg
    12601 atgggtctgg tcatcatggt ggtgatggcg caaattcctc cacgcgttta tgaaggctgg
    12661 gccccctatc tctatatcat ctgtattatt ttgctggtgg cggtagatgc tttcggtgcc
    12721 atctctaaag gcgctcaacg ctggctggac ctcggtattg ttcgttttca gccgtcggaa
    12781 attgccaaaa tagccgtacc actgatggtt gcgcgcttta tcaaccgcga cgtttgcccg
    12841 ccatcgttga agaacactgg catcgcgctg gtgctgatat ttatgcccac gctgctggtg
    12901 gctgcacagc ctgacctggg aacatcaatc ctcgttgcgc tttccggtct gtttgtactg
    12961 ttcctctctg gccttagctg gcgtctgatt ggcgtcgcag tagtgctggt agcggcgttc
    13021 attccgattc tgtggttctt cctgatgcat gattaccagc gccagcgcgt aatgatgctc
    13081 ctggacccgg aatcagaccc actcggcgcg ggctatcaca ttattcagtc taaaattgct
    13141 attggctccg gcggattacg cggcaaaggc tggctgcacg gcactcagtc acagcttgaa
    13201 tttctccccg aacgccatac cgactttatc ttcgcggtac tggcggaaga gctgggatta
    13261 gtgggcattt tgattctgct cgctctctac attctgctga tcatgcgcgg gctgtggata
    13321 gccgccagag cgcaaaccac ctttggtcgc gtcatggctg gcggcttaat gctgatatta
    13381 ttcgtttatg tcttcgtaaa tattggtatg gtaagcggta ttctgccggt tgtaggggtt
    13441 ccgctcccac tggtcagtta tggaggatcg gcgctaattg tgctgatggc tgggttcggg
    13501 attgtaatgt caatccacac ccacaggaaa atgttgtcga aaagcgtgta agaggtgcgc
    13561 aatgcgtaag cagtggctcg ggatctgcat cgcggcagga atgctcgcgg catgtacaag
    13621 cgatgatggt cagcaacaga cggtaagtgt accgcagcct gcggtatgta acggccccat
    13681 agttgaaatt agcggggcgg acccgcgttt cgaaccactg aacgcgacgg caaatcagga
    13741 ttaccagcgc gacggtaaaa gctacaaaat cgtgcaggat ccgtctcgat ttagccaggc
    13801 gggactggcg gcaatctatg atgccgaacc aggcagtaac ctgacggcct ctggcgaagc
    13861 tttcgatccg acacagctga cggcggccca tccaacgctt ccgatcccca gctacgccag
    13921 aatcactaac ctggctaacg ggcgaatgat cgtggtgcgc attaatgatc gcggccctta
    13981 cggcaacgac cgcgttattt cactttctcg cgcggcagct gaccgtctta acacgtcaaa
    14041 caacaccaaa gttcgtatcg atccgattat tgtcgcccag gatggttcgc tttctggtcc
    14101 tggtatggct tgtactacag tcgccaaaca gacttacgcc ctgcctgcac ctcccgattt
    14161 aagcggcggc gcaggaacaa gttcagtgtc tggcccgcag ggtgacattc ttccggtcag
    14221 taattcgacg ctaaaaagcg aagatccgac cggcgcgccg gtaaccagca gcggtttcct
    14281 cggcgcacca acgaccttag cgccaggtgt actggaaggc agcgaaccga cgcctgctcc
    14341 acagcccgtt gttacagctc cgtcgacaac gcctgcaacc tcgcctgcaa tggtgacacc
    14401 gcaagccacc tcgcaaagcg ccagcggcaa ctttatggtg caggtcgggg ccgtaagcga
    14461 tcaggctcgt gcgcaacagt accaacagca actgggacag aagttcggcg tccccggtcg
    14521 cgtaactcaa aatggcgcgg tctggcggat ccagcttggc ccattcgcca gcaaagccga
    14581 agccagtacc ttgcagcaac gtttgcaaac cgaagcccaa ttacagtcat ttattactac
    14641 cgcgcagtag cgtaaagcag gcatctgatg tgtcaatttc tgtaaatgac gttaacaaag
    14701 tcacgcggaa agtcagatgc ctgccggtag tgcatttgct atagtagggc acttttttaa
    14761 ttccatcacg gatgtcgtag ttcagaccat gaataccatt ttttccgctc gtatcatgaa
    14821 gcgcctggcg ctcaccacgg ctctttgcac agcctttatc tctgctgcac atgccgatga
    14881 cctgaatatc aaaactatga tcccgggtgt accgcagatc gatgcggagt cctacatcct
    14941 gattgactat aactccggca aagtgctcgc cgaacagaac gcagatgtcc gccgcgatcc
    15001 tgccagcctg accaaaatga tgaccagtta cgttatcggc caggcaatga aagccggtaa
    15061 atttaaagaa actgatttag tcactatcgg caacgacgca tgggccaccg gtaacccggt
    15121 gtttaaaggt tcttcgctga tgttcctcaa accgggcatg caggttccgg tttctcagct
    15181 gatccgcggt attaacctgc aatcgggtaa cgatgcttgt gtcgccatgg ccgattttgc
    15241 cgctggtagc caggacgctt ttgttggctt gatgaacagc tacgttaacg ccctgggcct
    15301 gaaaaatacc cacttccaga cggtacatgg tctggatgct gatggtcagt acagctccgc
    15361 gcgcgatatg gcgctgatcg gccaggcgtt gatccgtgac gtaccgaatg aatactcgat
    15421 ctataaagaa aaagaattta cgtttaacgg tattcgccag ctgaaccgta acggcctgtt
    15481 atgggataac agcctgaatg tcgacggaat caaaaccgga cacactgaca aagcaggtta
    15541 caaccttgtt gcttctgcga ctgaaggcca gatgcgcttg atctctgcgg tgatgggcgg
    15601 acgtactttt aaaggccgtg aagccgaaag taaaaaactg ctaacctggg gcttccgttt
    15661 cttcgaaacc gttaacccac tgaaagtagg taaagagttc gcttctgaac cggtttggtt
    15721 tggtgattct gatcgcgctt cgttaggagt tgataaagac gtgtacctga ccattccgcg
    15781 tggtcgcatg aaagatctga aagccagcta tgtgctgaac agcagtgaat tgcatgcgcc
    15841 gctgcaaaag aatcaggtcg tcggaactat caacttccag cttgatggca aaacgatcga
    15901 gcaacgcccg ctggttgtgt tgcaagaaat cccggaaggt aacttcttcg gcaaaatcat
    15961 tgattacatt aaattaatgt tccatcactg gtttggttaa aaattgaaca cttgaaagtg
    16021 taatttccgt ccccatatac taagcatcag taaaaaaact cccgccttct ggcgggagtt
    16081 gctatttaat tacgttacgc cggagctgac atgaaaacca aacttaacga actgcttgaa
    16141 ttccctactc cttttactta caaagttatg gggcaggcgt tacctgagct ggttgatcag
    16201 gtggttgaag tggtacagcg ccatgcgcca ggtgactaca ccccaacggt aaaaccaagc
    16261 agcaaaggca actaccactc ggtatctatc actatcaacg ccactcatat cgagcaggtt
    16321 gaaacactgt atgaagaact gggcaaaatc gatattgtcc gcatggttct gtaactcgct
    16381 tctccgttac ccggctcctg gtcgggtaac ccccctctcc tcgctgtgat atacttttcc
    16441 cccactttta ctcattctcc acggagatgc cgttttgtat caggataaaa ttcttgtccg
    16501 ccagctcggt cttcagcctt acgagccaat ctcccaggct atgcatgaat tcaccgatac
    16561 ccgcgatgat agtacccttg atgaaatctg gctggtcgag cactatccgg tattcaccca
    16621 aggtcaggca ggaaaagcgg agcacatttt aatgccgggt gatattccgg tgatccagag
    16681 cgatcgcggt gggcaggtga cttatcacgg gccggggcaa caggtgatgt atgtgttgct
    16741 taacctgaaa cgccgtaaac tcggtgtgcg tgaactggtg accttgcttg agcaaacagt
    16801 ggtgaatacc ctggctgaac tgggtataga agcgcatcct cgggctgacg cgcctggtgt
    16861 ctatgtcggg gaaaagaaaa tttgctcact gggtttacgt attcgacgcg gttgttcatt
    16921 ccacggtctg gcattaaacg tcaatatgga tctttcacca tttttacgta ttaatccttg
    16981 tgggtatgcc ggaatggaaa tggctaaaat atcacaatgg aaacccgaag cgacgactaa
    17041 taatattgct ccacgtttac tggaaaatat tttagcgcta ctaaacaatc ctgacttcga
    17101 atatattacc gcttaattgc atacatcaat ggcccaattt acactgggtc attactcata
    17161 atacctgcta atgctttaca tctccttcgc gtttgtatat tttctttatc acttgcagaa
    17221 atgcggtgct acgccttgct ccgtaactca ccccattgat taacttattg aaatagttga
    17281 ttatggtgag caaaagcgtt ccgttccaat tagacataag attattgtaa tttttttaaa
    17341 tattattttt ccatgaataa aattggagta agtgcgtgga tagtaataat caaatcgaac
    17401 cctgtttaag cagaaagtcg tcagaaggta aac
//
LOCUS       DAZUCE010000099        17428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZUCE010000099 DAZUCE010000000
VERSION     DAZUCE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.099"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            91..1326
                     /gene="moeA"
                     /locus_tag="ACHY03_002750"
     CDS             91..1326
                     /gene="moeA"
                     /locus_tag="ACHY03_002750"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFS3905959.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADITSGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     KVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGR"
     gene            1326..2075
                     /gene="moeB"
                     /locus_tag="ACHY03_002751"
     CDS             1326..2075
                     /gene="moeB"
                     /locus_tag="ACHY03_002751"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFS3905960.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(2254..2916)
                     /gene="fsa"
                     /locus_tag="ACHY03_002752"
     CDS             complement(2254..2916)
                     /gene="fsa"
                     /locus_tag="ACHY03_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFS3905961.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVLLP
                     ELHEAMGGQGRLFAQVMAITAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            3047..3946
                     /gene="ybiY"
                     /locus_tag="ACHY03_002753"
     CDS             3047..3946
                     /gene="ybiY"
                     /locus_tag="ACHY03_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFS3905962.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVDEIHFLPYHTLGINKYHLLNLPYGAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3952..6384
                     /gene="ybiW"
                     /locus_tag="ACHY03_002754"
     CDS             3952..6384
                     /gene="ybiW"
                     /locus_tag="ACHY03_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFS3905963.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLNSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6530..7345
                     /gene="ybiV"
                     /locus_tag="ACHY03_002755"
     CDS             6530..7345
                     /gene="ybiV"
                     /locus_tag="ACHY03_002755"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFS3905964.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7497..8762
                     /gene="ybiU"
                     /locus_tag="ACHY03_002756"
     CDS             7497..8762
                     /gene="ybiU"
                     /locus_tag="ACHY03_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFS3905965.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFSNVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPQLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(9003..10595)
                     /gene="ybiT"
                     /locus_tag="ACHY03_002757"
     CDS             complement(9003..10595)
                     /gene="ybiT"
                     /locus_tag="ACHY03_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFS3905966.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKMRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            10814..11734
                     /gene="ldtB"
                     /locus_tag="ACHY03_002758"
     CDS             10814..11734
                     /gene="ldtB"
                     /locus_tag="ACHY03_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFS3905967.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(11793..12911)
                     /gene="ybiR"
                     /locus_tag="ACHY03_002759"
     CDS             complement(11793..12911)
                     /gene="ybiR"
                     /locus_tag="ACHY03_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFS3905968.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLIQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(12908..13375)
                     /gene="mntR"
                     /locus_tag="ACHY03_002760"
     CDS             complement(12908..13375)
                     /gene="mntR"
                     /locus_tag="ACHY03_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFS3905969.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            13561..13689
                     /gene="mntS"
                     /locus_tag="ACHY03_002761"
     CDS             13561..13689
                     /gene="mntS"
                     /locus_tag="ACHY03_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFS3905970.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            13961..15544
                     /gene="opgE"
                     /locus_tag="ACHY03_002762"
     CDS             13961..15544
                     /gene="opgE"
                     /locus_tag="ACHY03_002762"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFS3905971.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(15592..16107)
                     /gene="ompX"
                     /locus_tag="ACHY03_002763"
     CDS             complement(15592..16107)
                     /gene="ompX"
                     /locus_tag="ACHY03_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFS3905972.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(16160..16225)
                     /locus_tag="ACHY03_002764"
     CDS             complement(16160..16225)
                     /locus_tag="ACHY03_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3905973.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            16460..17347
                     /gene="rhtA"
                     /locus_tag="ACHY03_002765"
     CDS             16460..17347
                     /gene="rhtA"
                     /locus_tag="ACHY03_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFS3905974.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         4218 a         4658 c         4406 g         4146 t
ORIGIN      
        1 acccagcgct acgcccgagg tggcatacag tctgtggcgt ttttctgaca taataggcaa
       61 attcgatttt gcctccgcag gagtgttttc atggaattta ccaccggatt gatgtcgctc
      121 gacaccgcgc ttaatgagat gctttctcgc gtcaccccac tgaccgccca ggaaacgctg
      181 ccactggttc agtgttttgg tcgtattctg gcgagcgatg tcgtttcgcc gctggatgtc
      241 ccagggtttg ataactccgc aatggatggt tacgcggtac gtttggccga tatcacctcc
      301 ggacaaccgc tgcccgttgc cgggaagtcg tttgccggtc agccttacca tggcgagtgg
      361 cccgctggca cctgcatccg cattatgact ggcgcaccag tacccgaagg ctgcgaagcg
      421 gtggtgatgc aggaacagac tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg
      481 cgtagcgggc aaaatattcg ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc
      541 ccggcgggga ctcgcctgac taccgcagag ctgccagtaa ttgcttcact ggggattgcc
      601 gaagttccgg tgattcgtaa agtgcgtgta gcgctttttt ctaccggtga tgaactccag
      661 ttgcccggtc agccgctggg cgacggccaa atctacgata ccaaccgtct cgccgtacac
      721 ctgatgttag aacagttggg atgcaaggta atcaacttag ggattatccg cgacgatcca
      781 catgccctgc gcgccgcatt tattgaagcc gacagccagg cggatgtggt gatcagttcc
      841 ggcggtgttt cagtgggtga agcggattac accaaaacga ttcttgaaga gctgggggag
      901 atcgccttct ggaagctggc gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc
      961 aatagctggt tctgcggcct gccgggcaac ccggtttcag cgacgctgac cttctatcaa
     1021 ctggtacagc ctttgctggc aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc
     1081 cagcgcgtac gcacagcgtc ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc
     1141 ggcgtgctgc aacgcaacgc cgatggcgaa ctggaagtga cgaccaccgg acatcagggt
     1201 tcacatatat tcagctcctt tagcctcggc aactgcttta tcgtgctgga acgcgatcgc
     1261 ggcaatgtgg aagtgggcga atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc
     1321 cggtaatggc ggaactcagc gatcaggaga tgctgcgcta caaccggcaa atcattctgc
     1381 gcggttttga ttttgacggt caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc
     1441 tcggtggact cggctgcgca gcctcgcagt atctggcaag cgccggtgtc ggcaacctga
     1501 cgctgctcga cttcgacacg gtttcgctct cgaatctgca acgccagacg ctgcacagtg
     1561 atgctacggt cgggcaaccg aaggtggaat ccgcccgtga cgccctggcg cggatcaacc
     1621 cacatatcgc gattacgcca gtcaatgcac tgctagatga cgcagaactt gcagcattga
     1681 ttgctgaaca cgatcttgtg ctcgactgta cggataacgt tgcggtacgt aatcaactga
     1741 acgcaggctg ttttgccgcg aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag
     1801 gtcaaatcac ggtcttcact tatcaggacg gcgagccttg ctatcgctgc cttagccgtt
     1861 tgtttggcga gaacgcgtta acctgcgttg aagcaggcgt gatggcaccg ttgatcggcg
     1921 tgattggttc gttgcaggca atggaagcga tcaaactgct ggcaggttat ggcaaaccgg
     1981 ccagcggcaa aatcgtcatg tacgatgcga tgacctgtca gtttcgcgaa atgaaactga
     2041 tgcgcaatcc ggggtgtgag gtgtgcgggc agtaattgcc acctccaggc ctgataagac
     2101 gcgacaagcg tcgcatcagg catttgctcc agacgccaga tgcggcgtga acgccttatc
     2161 tggcctacgg ttcgggcaca gatttccaag cgcggcaggc atcggtgcac tccgccacgt
     2221 catcagcaat ttatgaggat gcagaacgcc cggttaaatc gacgttctgc caaacgctcc
     2281 ctgccagtcc tgctcaaact tcgccacagc ggcatcaacc gccggatagc taatcatctg
     2341 ttgtgccaca tccagtggca gagtaattga ttcacatcct gccagtaagc agtccagcgc
     2401 ctgacgcggg gttttgaaac tcgcggctaa cactttcgcc tgcggcgcat gcattttcaa
     2461 taactggtgt aagtcggtca cagtctgaat gccgctaccg ccctgagcat caatacgatt
     2521 aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc
     2581 atataccgcg gttcccagcg tcggaatccc ttccgctttt aacatcttaa tcgctgccag
     2641 cccctcggcg gtcaccggaa ctttcaccac gatatccgca ataatagaac gcaatttacg
     2701 tgcgtcattc accatccctt cagcagtgat agccattacc tgggcaaaca gacgcccctg
     2761 accgcccatc gcttcatgaa gttccggaag caaaacctcc agcggttttt tacctgcggc
     2821 gataatgctt gggttagtgg tcacgcccgc cagcggaaaa atacgtgaca gcgccttcac
     2881 cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa tgtacttttt
     2941 gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat caatctaact
     3001 ttcattcgaa agtattttaa tgtttgaacg aaacagagag gcaactatga ttttcaatat
     3061 tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc ttaaaggctg
     3121 ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgcacgc aggatctgct
     3181 gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggcag cgccggaagt
     3241 gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaacac cggagcatct
     3301 gacggcgtta accgactgct gcccgacaca ggcattaacc gtgtgtggtg aagtgaaaag
     3361 cgttgaggag atcatggcga ccgttttgcg ggataaaccg ttttacgatc gcagcggcgg
     3421 aggcttaacg ctttcgggtg gtgaaccctt tatgcagccg gaaatggcga cggcgctgct
     3481 gcaagccagc cacgaggccg gcattcatac tgcggtagaa acctgtctgc atgtgccgtg
     3541 gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt taaaacacgt
     3601 tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagggtgc tggataacct
     3661 gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccactga ttcagggctt
     3721 taatgccgac gaaacctctg taaaagccat taccgatttt gccgccgacg agctgcacgt
     3781 tgacgaaatt catttcctgc cctaccacac gctgggcatc aacaaatatc acttacttaa
     3841 tctgccctat ggcgccccgg aaaaaccgct tgatgcgcca gaactgctcg actttgccca
     3901 gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac catgaccaca
     3961 ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt
     4021 gtgaaaccgc cggtttgtac cgagcgcgcg cagcactata ccgagatgta tcaacaacat
     4081 ctcgataagc cgatccctgt acgtcgcgcg ctggcgctgg cacatcacct ggcgaatcgc
     4141 actatctgga tcaagcacga tgagttgatc atcggcaacc aggcaagcga agttcgcgcc
     4201 gcgccgatct tcccggaata tactgtctcg tggatcgaaa aagagattga tgatctggca
     4261 gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgcgttct gcatgaagtg
     4321 tgcccgtggt ggcgcggtca gaccgtacag gatcgctgct acggcatgtt taccgatgag
     4381 caaaaaggtc tgctggcgac cggcatcatt aaagcggaag gcaatatgac ctccggcgat
     4441 gcgcacctgg cggtcaattt cccgctgctg ctggaaaaag ggcttgatgg tctgcgcgag
     4501 aaagtagcgg aacgtcgctc gcgcatcaac ctgacggtgc tggaagattt acacggtgag
     4561 caattcctga aagcgattga tatcgtgctg gtggcagtca gtgaacatat tgaacgtttc
     4621 gctgccctgg cgcgtgaaat ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg
     4681 ctggcgatag cagaaaactg cgatcttatc gcccaccagc cgccgcagac tttctggcag
     4741 gcgctgcaac tgtgttactt catccagttg atattgcaga ttgaatctaa cggtcactcg
     4801 gtgtcgtttg gtcgtatgga tcagtatctc tatccgtact atcgccgcga cgttgaactc
     4861 aaccagacac tggatcgcga acacgccatc gaattgctga atagctgctg gctgaaactg
     4921 ctggaagtga acaaaatccg ctcaggctca cactcaaaag cctctgcggg aagtccgctg
     4981 tatcagaacg tcaccattgg cgggcaaaat ctggttgatg gtcaaccaat ggacgcggtg
     5041 aatcccctct cttacgcgat ccttgaatcc tgcggtcgcc tgcgttccac tcagcctaac
     5101 ctcagcgtgc gctaccacgc gggaatgagc aacgatttcc tcgacgcctg tgtgcaggtg
     5161 atccgctgcg gcttcgggat gccggcgttc aacaacgacg aaatcgtgat cccggaattt
     5221 attaaactcg gtattgaacc gcaggacgct tacgactacg cagcgattgg ttgtatagaa
     5281 accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat caacttcgcc
     5341 cgcgtgatgc tggcggcgct ggaaggtggt cgggatgcca ccagcggcaa agtgttcctg
     5401 ccacaagaaa aagcgttgtc agcgggtaac tttaacaatt tcgatgaagt aatggacgcg
     5461 tgggatacgc aaatccgtta ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac
     5521 accatgctgg aagagaacgt gcacgatatt ctctgctcgg cgctggtgga tgactgtatt
     5581 gagcgagcga aaagcatcaa gcaaggcggc gcgaaatatg actgggtttc tggcctgcag
     5641 gttggcattg ccaacctcgg caacagcctg gcggccgtga agaaactggt atttgaacag
     5701 ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga cggcctgact
     5761 catgagcaac tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa cgacgatgat
     5821 actgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact gaaacagtac
     5881 cataacccgc gctacggtcg tggtccggtt ggcggcaact attacgcggg tacgtcgtct
     5941 atctccgcta acgtaccgtt tggcgcgcag actatggcaa caccggacgg gcgtaaagcc
     6001 cacaccccgc tggcagaagg cgcaagcccg gcttccggta ctgatcatct tggccctact
     6061 gcggtcattg gctcggtggg taaactgcct acggcagcga ttctcggcgg cgtgttgctc
     6121 aaccagaaac tgaatccagc cacactggag aacgaatctg acaagcagaa actgatgatc
     6181 ctgctgcgca ccttcttcga ggtgcataaa ggctggcata ttcagtacaa catcgtttcc
     6241 cgcgaaacgc tgctggatgc gaaaaaacat cccgatcagt atcgcgatct ggtagtgcgt
     6301 gtcgcgggtt attccgcctt cttcaccgcg ctctctccag acgctcagga cgatatcatc
     6361 gcccgtaccg aacatatgct gtaatcccct cagcccggcg gagacgtcgc cgggctaaat
     6421 cattcacacc atcaatttca ctctcacttt cattcgaaat ataatttgtg ctctgcgtca
     6481 cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta tgagcgtaaa
     6541 agttatcgtc acagacatgg acggtacttt tcttaacgac gccaaaacgt acaaccaacc
     6601 acgttttatg gcgcaatatc aggaactgaa aaagcgcggc attaagttcg ttgtcgccag
     6661 cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg agatctcttt
     6721 tgtcgcggaa aacggcgcac tggtttacga acatggcaag caactgttcc acggcgaact
     6781 gacccgacat gaatcgcgga ttgttattgg cgagttgcta aaagataagc aactcaattt
     6841 tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag cattcgtcgc
     6901 actgatggca aaacactacc atcgcctgaa acctgtaaaa gattatcagg agattgacga
     6961 cgtactgttc aagttttcgc tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa
     7021 actgcacata gcgctggatg gcattatgaa acccgtcacc agtggttttg gctttatcga
     7081 cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga aacgctggga
     7141 tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg agatgctgaa
     7201 aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg
     7261 ttacgctacc gatgataata atcatgaagg cgcgctgaat gtgattcagg ctgtactgga
     7321 taacacatcc ccttttaaca gctgacctct ctaccggagc catgcgctcc ggttttccct
     7381 tctttttaca tcctgtacat cacatcaaat cattaaactt gcattaactt atttataact
     7441 tatattgtca catgcaaatc agcatagttt cgaatgaaag ttaatgcgag gtggttatgg
     7501 cttctacttt taccagcgac acattgcctg ccgatcacaa agcagctatc cgtcagatga
     7561 agcacgcgct gcgggcgcag cttggcgacg tccagcagat ctttaatcag ctaagcgatg
     7621 acattgccac gcgagtggct gaaatcaacg cactcaaagc acagggcgat gccgtctggc
     7681 cggtgctgtc ttatgccgat atcaaagcag gtcatgttac tgcagagcag cgcgaacaga
     7741 ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa gcgctaggct
     7801 gggatcagtc gatgctggac tatctggacc gcaaccgctt tgacgaggtc tacaaaggcc
     7861 ccggcgataa tttcttcggg acgctcagcg cttcacgtcc cgagatttac cccatctact
     7921 ggtcgcaggc gcaaatgcag gctcgccaga gtgaagaaat ggcgaatgcg cagtcgtttc
     7981 tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg
     8041 tcatctaccc tgaccgtatc cgccgccgtc cgcccggaac gacctccaaa ggtcttggag
     8101 cgcataccga ctccggggca ctggaacgct ggctgcttcc agcgtatcag cgcgttttct
     8161 ccaacgtctt taatggcaat ctggcgcaat atgatccctg gcatgcggca catcgtacgg
     8221 aagttgaaga gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg acattccagg
     8281 gctggacagc gctctctgat atgctgcctg gtcaggggct gctgcacgtc gtgcccattc
     8341 ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac
     8401 tgtgcggcgt agcgcccgga agagtattgc cggtatcaga gcaatggcat ccactgttga
     8461 ttgaggcatt aaccagcatt ccacaactcg aagccggaga ctccgtctgg tggcactgcg
     8521 acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg ctggggcaac gtgatgtaca
     8581 ttcctgcggc accgatgtgc gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac
     8641 tggaaaaagg cgcatcgccg ggcgatttcc cgcgcgagga ctatgaaaca aactgggaag
     8701 gacgctttac gcttgccgac ctcaacattc acggtaagcg agcgttgggc atggatgttt
     8761 gagagttaat ggaagtgaat tgatgtggga ttaagaaaat caattcgacg ttactcagat
     8821 tgctgacaag tgcgctttct taatgcctga tgcggcgtta acgccttatc tggcctacaa
     8881 agtcctgcag attcaataca ttacaggtac tgcgtaggcc tgataagcat agcgcatcag
     8941 gcaatcttgc gtttgtcatc agccgccgtc accccgacag tatcggggtg acggataata
     9001 acttactcga tccctttact acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg
     9061 cgttccgggg tgatttccag aatgcgggtc gccagggagc ttacgaactc acggtcgtgt
     9121 gaaacaaaga tcagcgtgcc ctgatacagt tccagcgcca tgttcagtga ctcgatggat
     9181 tccatatcca ggtggttggt cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt
     9241 aacttaccaa aaagcatccg ccctttttct ccaccggaaa gcactttagc aggctttttg
     9301 atgtcgtcct ggctgaacag caaacgaccg agaatactgc gcaccgcctg ctcgtcatcg
     9361 ccttcctgct tccactggct catccattcg aacacggtca gatcattttc aaactcatat
     9421 tcgtggtcct gagcatagta accaatgcgc gcgttttcag accattttac ggtgccgctg
     9481 tccggttgca gatcgcccac cagcgttttc aacagcgttg atttaccgac gccgttggta
     9541 cccagtaccg ccagtttttc accgacttcc agcagcaggt tgagattttt aaacagcgga
     9601 ccgttatcaa accctttggt cagaccttcc acttccagcg cgttacggaa cagcttctta
     9661 tcctgttcaa aacggatgaa cgggttctga cggctggagg ctttaacctc ttccagtttg
     9721 attttatcaa tctggcgcgc gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta
     9781 aagcggctaa cgaaagattg cagctcagca atctgcgctt tcttcttggc gttatcagcc
     9841 agcagacgtt cacgcgcctg ggtcgccgcc gtcatgtact catcgtagtt gcccggataa
     9901 acgcgcagct cgccgtaatc cagatccgcc atgtgggtac agaccatgtt aaggaagtga
     9961 cggtcgtgcg agatgatgat catggtgctg tcacgctcgt tcagcacctg ttccagccag
    10021 cgaatggtgt cgatgtcgag gttgttggtc ggttcgtcga gcaggagaat gtccggatca
    10081 gcaaacagcg cctgcgccag caacacacgc agcttccagc caggagcaac ttcactcatc
    10141 gggccgtagt gctgttccac tggaattccc acgccaagca acaattcacc ggcacgagct
    10201 tccgcagagt aaccgtccat ttcgccgtat ttaacttcca gatcggccac tttatagccg
    10261 tcttcttcac tcatttccgg caaagcatag atgcggtcgc gctcctgctt cacttcccac
    10321 aactctttat gccccatgat caccgtatcc agcacagtga actcttcaaa ggcaaactga
    10381 tcctgacgca tcttaccaat gcgctcgttg ggatcgaggg aaacgttacc caacgtcggc
    10441 tctaagtcgc cgccgaggat cttcataaag gtggatttac cactaccgtt cgcgccaatc
    10501 aggccgtaac ggttgccgcc gccaaatttg acggaaatgt tttcaaacaa cggcttactg
    10561 ccgaactgca tggtgacgtt actggaaact aacacaggcg tatcctgaaa agagatatga
    10621 caaaccgcgc attatgccat aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca
    10681 ctcaagccaa aggcaaaaaa tgtgatttcg tacacatctg atttcactgt gagctggaat
    10741 gaacttataa tgcgcttcca atactctaat attctcaacc caatggcctg ccaggcacaa
    10801 aatctcgctt aacatgaata tgaaattgaa aacattattc gcagcggcct tcgctgttgt
    10861 cggcttttgc agtaccgcct ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct
    10921 ggttggtcag aatcaggtga tcaccattcc tgaaggtaac actcagccgc tggagtattt
    10981 tgccgcggag taccagatgg ggctttccaa tatgatggaa gcgaacccgg gtgtggatac
    11041 cttcctgccg aaaggcggta ctgtactgaa cattccgcag cagctgatcc tgccggatac
    11101 tgttcatgaa ggcatcgtca ttaacagtgc agagatgcgt ctgtattact atccgaaagg
    11161 gaccaacacc gttatcgtgc tgccgatcgg cattggtcag ttaggcaaag atacgcctat
    11221 caactggacc accaaagttg agcgtaagaa agcaggcccg acctggacgc cgaccgccaa
    11281 aatgcacgca gagtaccgcg ctgcgggcga accgcttccg gctgtcgttc cggcaggtcc
    11341 ggataacccg atggggctgt atgcactcta catcggtcgc ctgtatgcta tccatggcac
    11401 caacgccaac ttcggtatcg gtctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga
    11461 agacatcaaa ttcctgttcg agaaagtacc ggtcggtacc cgcgtacagt ttattgatga
    11521 gccggtaaaa gcgaccaccg agccagacgg cagccgttat attgaagtcc ataatccgct
    11581 gtctaccacc gaagcccagt ttgaaggtca ggaaattgtg ccaattaccc tgacgaagag
    11641 cgtgcagaca gtgaccggtc agccagatgt tgaccaggtt gttcttgatg aagcgatcaa
    11701 aaatcgttcc gggatgccgg ttcgtctgaa ttaatcgtca aaacgtaaaa caaaggcgga
    11761 ctcataatcc gccttttttt atttgccaga ccttaccagg ccgggatcat aaccaacaaa
    11821 acatatccca ccagcgccgc ccacaacagc atcggtattg aatagagatg gaaacgccac
    11881 cagatgcggc gatcgttcgc catacgtagc gcaatcaaat ttgccagcga tccgggtaat
    11941 aacccaaagc cacctacgtt taccgcccat gccagtaata aagacggcgg cacatagttc
    12001 agcaacaata tggtactcgg cacgttactg atcacctgcg ataaaccgat tgccgttaac
    12061 cataacccgg gttcagataa atgactcacg ttacccaaca cgccttgcaa cgctggaagc
    12121 tggatcagta aatggacgtc gataaacatc gccataaaca ccagcagcaa cgtccagtcc
    12181 acactcaaca ccacgcgacg cgccagcagc gcaaagcccg ccgccacaat caccagtccc
    12241 cacagctctt gtttgaactc cagcgccgtc agaaagacga tatacagccc cagacaactc
    12301 cacaccagcc gcggtttcca ctccggtgtt tgcacccccg tatggtattg gagtgccttt
    12361 ccagggaaac aacaccagca caacagcagg agtgtcagca tcattgcgcc agccagcggt
    12421 gccatttggg caataaatcc ggcaaacgaa agaccagaac gtccccagat aagaatattt
    12481 tgcgggttgc caattggcgt cagtagcgaa ccagcgttga ctgccagcgc ctcaaaaata
    12541 atcagccgat taaccgggat ctcacacagc ctttttagcg tgatagtcag cggaacaaca
    12601 ataaacagcg cgacatcgtt ggtcagaaag gtagaaagca gcgccgccgc cagcaccata
    12661 aacatcgcca gccgacgctc cgtagcaaag cggcgcacca ttttgcgccc cagcacatca
    12721 aaataaccgc ttaactccac acctttggtc agcagcatca ggccgcttaa ggtgatgatg
    12781 gtgtgccagt cgatagcagc aggccaagat ttcggtgcaa agggaacgaa aaaacttaat
    12841 ccgataccaa caagaattaa taactgaaaa aaacgatcgc cttgcagcgt gcgtaaaaaa
    12901 ggcaggctca tttggcaccg tgtttctggg taaacaaacg aaaggcatcc agcgtctctt
    12961 cactaacatg gtgctccatg ccttccgcgt cgcgacgggc gatttccgga ctgacgccca
    13021 acaccaacaa gaaattttcg actatctgat gtcgctcgcg gctttcctgc gccagcttct
    13081 ctccttctgc cgttaaaaac acgccacgcc aggggatcat ttcaatcagc cccatagttg
    13141 ccagtcgctt aagcatttta gccaccgtcg gttgcgaaac tcccaaacga gcagccatgt
    13201 ctacctggcg agcttccccc acttccctga tcaagtcaga aatcagctca acgtaatcat
    13261 caataagctc gcgccgatgc gcctctctga cctggcggaa cccttcaacg tgctcttcca
    13321 cgttcactaa ctgcgtcact ttttttgctg ttggcgtacc tgcgcgacga ctcattgtgc
    13381 ttcctcatta tggtgacgca tacatagcgt tctcatttcg tgcgcacatt gtaaaccaga
    13441 gttgcgaagg tacaaaaaat taacgtttta gcaatagcta tataatatag cctgtgctat
    13501 atctgtatgt aatgcaatca tccctcaagg atcgacggga ttagcaagtc aggaggtctt
    13561 atgaatgagt tcaagaggtg tatgcgcgtg tttagtcatt ctccctttaa agtacggtta
    13621 atgctgctct ctatgttgtg cgatatggtc aacaacaaac cgcagcaaga taaaccttcc
    13681 gataaatagc ggcgtcgcgg tacgccgctt ccctcctgct ttcatgcagg cataacgcgt
    13741 tttggtctga aaaaccccac tttttgtcgg atttgcaatc cccttcgcaa aagatttgtt
    13801 cgtcagtagt tgacctgaac ggcggctcgc tctatcttct tgcagccctg cgtatattgc
    13861 ggctcgcgga tgcggacccc tttcccctct tcacgcactc ttgcaggtat tgacccttga
    13921 cgccagggta agcacatggc gtttgttacg atagtggcat atgaatttaa ccctcaaaga
    13981 atcgcttgtt acccgtagcc gggtatttag cccgtggact gcgttctact ttttacagtc
    14041 gctattaatt aacctcggct taggttaccc cttcagtttg ctctacaccg ctgcgtttac
    14101 ggctattttg cttttgctat ggcgaacatt gcctcgcgta caaaaagttc tggtcggtgt
    14161 cagttcgctg gtggcggctt gttatttccc ttttgctcag gcctacggcg cgcctaattt
    14221 caatacattg ctggcattgc actccaccaa tatggaagag tcgaccgaaa tcctgacgat
    14281 ttttccgtgg tacagctacc tggtcggctt atttattttt gcgctcggcg taatagcaat
    14341 caggcgaaaa aaagagaatg aaaaagcgcg ctggaatacc ttcgacagcc tgtgtctggt
    14401 attcagtgtg gcgacatttt ttgttgctcc cgtgcaaaac ctggcctggg gtggcgtatt
    14461 taaactgaaa gatactggct atccggtatt tcgttttgct aaggatgtca tcgtcaataa
    14521 taacgaggtg attgaagagc aagaacggat ggcaaaactt tccggaatga aagatacctg
    14581 gacggtcact gccgttaagc cgaagtatca gacctatgtg gtggtgatcg gtgaaagcgc
    14641 gcgtcgcgat gccctcggtg cctttggcgg tcactgggac aataccccgt ttgccagcag
    14701 cgttaacggt ttgatatttg ctgactacat tgccgccagt ggctccacgc agaaatcgct
    14761 tggcttaacg ctcaatcgcg ttgtcgatgg caagccacag tttcaggata actttgtcac
    14821 cctggcaaat cgcgcgggct tccagacctg gtggttttcc aaccagggtc aaatcggcga
    14881 atacgataca gccatcgcca gcatcgccaa acgagcagat gaagtgtact tcctgaaaga
    14941 aggtaatttt gaagcagata aaaacaccaa agacgaagcg ttactggata tgaccgctca
    15001 agtgctggcg caagagcact cgcaaccgca gctgattgtt ctgcatctga tgggctcgca
    15061 tccgcaggcc tgcgacagga cacaaggaaa atacgaaacc tttgtgcaat cgaaagaaac
    15121 gtcgtgctat ctctatacca tgacgcaaac agacgattta ctgcgcaagc tgtacgatca
    15181 gttacgcaac agcggcagca gcttctcgct ggtttacttt tctgaccacg gtctggcctt
    15241 taaagagcgc ggcaaagacg tgcaatacct tgcccatgat gataagtatc agcaaaattt
    15301 ccaggtgcct tttatggtca tttccagcga cgataaagcg catcgtgtga ttaaagcccg
    15361 ccgctcagcc aatgacttct taggcttttt ctcccagtgg acgggaatta aagcgaagga
    15421 aattaacatc aaatacccgt ttatatctga gaagaaagcc gggccgatat acatcaccaa
    15481 cttccagtta cagaaggtgg attacaacca tctcggaacc gatattttcg acccgaaacc
    15541 ttaaaacaaa aatccgcccc gagaggcgga ttttttatat caccgaagtg attagaagcg
    15601 gtaaccaaca ccggcaatcc aggtgcctac gtcaacgcta cgaatacggc tctgctcgta
    15661 agagaagtcc agagcaacgt tttccatcgg gttgaactgc agaccagcac cgtaggagaa
    15721 accgtagtcg ctggtgtcgt gtttgtaggt cgggtattca gtggtctgga atttaccata
    15781 acccacaccc actacaccgt agatgcttgc ccagtcgtta atgcggtaag ccggaccagc
    15841 agtgatgccg tagtactggt ttttgttgta gtcaccagag cttgcagtac ggcttttctc
    15901 ggtgtaagtg aaagaaccga tcacacccag cgggctgttg tcttcttcat agcggtattt
    15961 caggttgaaa ccgcccattt tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc
    16021 agttacggta gaagtcgcag ctacggaagt acctgcggtg aaagccagaa ctgcggccag
    16081 tgctgaaaga catgcaattt ttttcataac cacctcaaat gtgattcaaa taagtcctaa
    16141 gttttaaata tatcaaaaat taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc
    16201 atttcatgta acagaacgtt tccatacacc gctatccatc taaatttaaa tcactttttc
    16261 agagaactgc gtaagtatta cgcatgtttt ccctgtcatt catccagatt attcctaatc
    16321 accagactaa tgattccatc aatcctggcg cattttagtc aaaacggggg aaaatttttt
    16381 caacaaatgc tcaaccagca ttgggtatat ccagtacact ccacgcttta cttaagtcta
    16441 gatatttgtg ggagaaagga tgcctggttc attacgtaaa atgccggtct ggttaccaat
    16501 agtcatattg ctcgttgcca tggcgtctat tcagggtgga gcttcgttag ctaagtcact
    16561 ttttcctctg gtgggcgcac cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat
    16621 cctcatcgcg ttctttaagc catggcgact gcgctttgcc aaagagcaac gattaccgct
    16681 gttgttttac ggcgtttcgc tgggggggat gaattatctt ttttatcttt ctattcagac
    16741 ggtaccgctg ggtattgcgg tggcgctgga gttcaccgga ccactggcgg tggcgctgtt
    16801 ctcttctcgt cgcccggtag atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt
    16861 cctgctacct ctggggcaag acgtttccca tgtcgattta accggctgtg cgctggcact
    16921 gggggccggg gcttgttggg ctatttacat tttaagtggg caacgcgcag gagcggaaca
    16981 tggccctgcg acggtggcaa ttggttcgtt gattgcagcg ttaattttcg tgccaattgg
    17041 agcgcttcag gctggtgaag cactctggca ctggtcggtt attccattgg gtctggcggt
    17101 cgctattctc tcgaccgctc tgccttattc gctggaaatg attgccctca cccgtttgcc
    17161 aacacggaca tttggtacgc tgatgagcat ggaaccggcg ctggctgccg tttccgggat
    17221 gattttcctc ggagaaacac tgacacccat acagctgctg gcgctcggcg caattatcgc
    17281 cgcttcaatg ggttctacgc tgacagtacg caaagagagc aaaataaaag aattagacat
    17341 taattaaatt tacatttctg catggttatg cataaccatg cagaatttct cgctactttt
    17401 cctctacacc gtctttatat atcgaatt
//
LOCUS       DAZUCE010000100        17128 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZUCE010000100 DAZUCE010000000
VERSION     DAZUCE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.100"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            132..386
                     /gene="bssS"
                     /locus_tag="ACHY03_002766"
     CDS             132..386
                     /gene="bssS"
                     /locus_tag="ACHY03_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFS3905975.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            501..1619
                     /gene="solA"
                     /locus_tag="ACHY03_002767"
     CDS             501..1619
                     /gene="solA"
                     /locus_tag="ACHY03_002767"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFS3905976.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCSVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1667..1780
                     /locus_tag="ACHY03_002768"
     CDS             1667..1780
                     /locus_tag="ACHY03_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFS3905977.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2041..2607
                     /gene="yceJ"
                     /locus_tag="ACHY03_002769"
     CDS             2041..2607
                     /gene="yceJ"
                     /locus_tag="ACHY03_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFS3905978.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2611..3186
                     /gene="yceI"
                     /locus_tag="ACHY03_002770"
     CDS             2611..3186
                     /gene="yceI"
                     /locus_tag="ACHY03_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFS3905979.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3228..4280)
                     /gene="trhO"
                     /locus_tag="ACHY03_002771"
     CDS             complement(3228..4280)
                     /gene="trhO"
                     /locus_tag="ACHY03_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFS3905980.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4505..5425
                     /gene="lpxL"
                     /locus_tag="ACHY03_002772"
     CDS             4505..5425
                     /gene="lpxL"
                     /locus_tag="ACHY03_002772"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFS3905981.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5597..6823
                     /gene="mdtG"
                     /locus_tag="ACHY03_002773"
     CDS             5597..6823
                     /gene="mdtG"
                     /locus_tag="ACHY03_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFS3905982.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6906..7280
                     /gene="msyB"
                     /locus_tag="ACHY03_002774"
     CDS             6906..7280
                     /gene="msyB"
                     /locus_tag="ACHY03_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFS3905983.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7281..7508)
                     /gene="yceK"
                     /locus_tag="ACHY03_002775"
     CDS             complement(7281..7508)
                     /gene="yceK"
                     /locus_tag="ACHY03_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFS3905984.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7681..10224)
                     /gene="mdoH"
                     /locus_tag="ACHY03_002776"
     CDS             complement(7681..10224)
                     /gene="mdoH"
                     /locus_tag="ACHY03_002776"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFS3905985.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHIEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10217..11752)
                     /gene="mdoG"
                     /locus_tag="ACHY03_002777"
     CDS             complement(10217..11752)
                     /gene="mdoG"
                     /locus_tag="ACHY03_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFS3905986.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPLGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12145..13302
                     /gene="mdoC"
                     /locus_tag="ACHY03_002778"
     CDS             12145..13302
                     /gene="mdoC"
                     /locus_tag="ACHY03_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFS3905987.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSLIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13310..14791)
                     /gene="clsC"
                     /locus_tag="ACHY03_002779"
     CDS             complement(13310..14791)
                     /gene="clsC"
                     /locus_tag="ACHY03_002779"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309451.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFS3905988.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEIAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIVLKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14733..15266)
                     /gene="ymdB"
                     /locus_tag="ACHY03_002780"
     CDS             complement(14733..15266)
                     /gene="ymdB"
                     /locus_tag="ACHY03_002780"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFS3905989.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15361..15672)
                     /gene="ymdA"
                     /locus_tag="ACHY03_002781"
     CDS             complement(15361..15672)
                     /gene="ymdA"
                     /locus_tag="ACHY03_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFS3905990.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPDKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15793..16125)
                     /gene="csgC"
                     /locus_tag="ACHY03_002782"
     CDS             complement(15793..16125)
                     /gene="csgC"
                     /locus_tag="ACHY03_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFS3905991.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16184..16642)
                     /gene="csgA"
                     /locus_tag="ACHY03_002783"
     CDS             complement(16184..16642)
                     /gene="csgA"
                     /locus_tag="ACHY03_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFS3905992.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSHMTVKQFGGGNGAAVDQTASNSTVNVTQVGFGNNATAHQY"
     gene            complement(16683..17126)
                     /gene="csgB"
                     /locus_tag="ACHY03_002784"
     CDS             complement(16683..17126)
                     /gene="csgB"
                     /locus_tag="ACHY03_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFS3905993.1"
                     /translation="MLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFNQAAI
                     IGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDASISQ
                     GAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4294 a         4367 c         4066 g         4401 t
ORIGIN      
        1 agaaatttat ctgtttcccc ctttaaaaat tcggtgaata cccttactta ttggtatact
       61 gaagttgctt cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt
      121 agggggccat gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg
      181 acatcagcac cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa
      241 ataagtccga gcaggaaggg actgaagttg gtcagacgct ctggttaacc actgatgttg
      301 ccagacaatt tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc
      361 aggtaaacga gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac
      421 cggtgccttt accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc
      481 atgagagcag agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag
      541 gcgctgccgc cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc
      601 atatgccacc acatcaacac ggcagccacc acggcgatac gcgattaatt cgccatgctt
      661 atggtgaagg cgaaaagtat gtcccgctgg tcctccgcgc acaaacgctg tgggatgacc
      721 tctcccgcca caacgaagat gatcccattt ttgtacgctc tggtgtcatt aaccttggcc
      781 cggctgactc cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg
      841 ttgaaaagct cgatgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca
      901 actacatcgg cttatttgag actgattccg gctttttacg cagcgaactg gcgattaaaa
      961 cctggatcca actggcgaag gaagcgggct gtgcgcaact gttcaactgc tcggtcaccg
     1021 caattcgtca tgacgatgat ggcgtaacta ttgaaacggc tgacggtgag tatcaggcga
     1081 aaaaagcgat tgtctgcgcg ggaacatggg taaaagacct gctcccggag ctgcctgtcc
     1141 agcccgtacg taaagtattt gcctggtatc aggccgatgg ccgctatagc gtgaagaata
     1201 aattcccggc gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag
     1261 aaaacgacgc gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg
     1321 aacgtgttcc gtttgcggaa gtggccagtg atgggtcaga agccttcccg ttcttgcgca
     1381 atgtattgcc gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc
     1441 ctgacgaaga ttttattatc gataccctac ccggccacga taatacactg ctcattaccg
     1501 gcctgagtgg gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg
     1561 cgcaagacaa aaaaagcgat tttgatttga cgccattcag gctttcccgc ttccaataat
     1621 catcattacg gcctccagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt
     1681 gcactatctc atcaataata ttcgcgagca tctgatgctt tatctttttc tctggggatt
     1741 gttggcaatt atggatctga tctatgtgtt ttatttttag ttttccgcac tgacataaac
     1801 tgacaaatat ttaatctccc tttcatttag cgtgaaaaat tgaatgagat tattcctgct
     1861 tctacagcac tcaccagaca tctctgatgc tgaacccact aaacttatga agattctttc
     1921 attattaatt caaaattatt gaaataaaaa taaatatcat tcaatttgtt atttttgtgt
     1981 tgcaattcat tctccgacac gccattttct ttctctcgca aattcgtttt ggatgcactt
     2041 atgtcattca caaatacccc tgaacgctat ggtgttatat ctgcggcctt ccattggcta
     2101 agcgcaatta ttgtctacgg catgtttgct ttaggtctgt ggatggtcac gctcagttat
     2161 tacgatggct ggtatcacaa agcacccgaa ctgcataaaa gcatcggtat tttattaatg
     2221 atggggctgg ttattcgcgt tctgtggcgt gtcatatctc caccgccggg tccgctgccg
     2281 agttattcgc ccatgactcg tcttgccgca aaagctggac atctcgcatt gtacctattg
     2341 ctgttcgcca tcggcatcag cggctatctg atctcaactg ccgatggtaa accgatcagc
     2401 gtttttggct ggtttgacgt ccccgcgacc cttgccgacg ctggcgcaca ggcagacttt
     2461 gccggtgccc tgcatttttg gctcgcgtgg agcgtcgtag tactgtccgt tatgcacgga
     2521 tttatggccc tgaagcatca tttcatcgat aaagacgaca ctctgaagcg catgctggga
     2581 aagtcgtcat ctgactatgg agtataaaaa atgaaaaaaa gcctgcttgg tttaaccttc
     2641 gcgtccctga tgttctctgc cggttcagcg gttgccgccg attacaaaat tgacaaagaa
     2701 ggtcagcacg cctttgttaa cttccgcatc cagcatcttg gctatagctg gttatatggc
     2761 acctttaaag atttcgacgg tacttttacc tttgatgaaa aaaatccagc tgccgataaa
     2821 gtgaatgtga caattaacac caccagcgtc gatactaacc acgccgaacg cgataaacat
     2881 cttcgcagtg cggatttcct caataccgca aaatatccac aggcaacatt cacctccacc
     2941 agcgtgaaga aagacggtga cgaactggat attaccggcg atctgacgct gaatggtgta
     3001 accaaacccg tcacgctgga agcgaaatta attggtcagg gcgacgaccc gtggggtggt
     3061 aaacgtgcag gctttgaggc cgaaggcaaa attaagctca aagacttcaa tatcaagaca
     3121 gatttaggtc cagcttctca ggaagtggat ctgattattt cagtggaagg cgtacagcag
     3181 aagtaagagt gaaatgttgc cggatggcac gcatccggca atgatattta ttctgttgga
     3241 tcaggaatgc ccagtgttgt attcagacgt ccacgagact tattaaatat cttattgcca
     3301 ttttcacgtc ctgcccgacg gcgtcgctgt tcttctggcg gtaacgcgct ttcttcgcag
     3361 caaatttcac tacaacaacc tttgtatttt tccgcacata ctggacactg aataaacagc
     3421 aggtggcagc catcattttt acagttggta tggctgtcgc acggcgcacc gcactggtgg
     3481 caatgcgcga taatctcatc agatatacgt tcgcccatcc gctcgtcaaa aacaaaattt
     3541 ttgccaataa aacgcaccgg caagccctgc tcgcgcgcct tacgggcgta ttcaataatt
     3601 ccgccctcga tatgccagac tttgttgaat ccgttatgtt tcatccaggc actggccttt
     3661 tcacaacgaa tgccgccggt gcagtacata acgatttttt tatctttatg tgcctgcatc
     3721 atctcgactg cttttggcag ctgctcacgg aaagtatctg ccggaatttc cagcgcgttt
     3781 tcaaagtgcc ccacttcata ctcatagtgg ttacgcatgt cgataaatag tgcatcggga
     3841 tcgtcaagca tggcgttcac ttccgccgct tgcagatact caccaacatt gctggcatca
     3901 aagtgaggat cgtcaatacc gtcggcaaca atgcgatcgc gtaccttcat gcgcagtacc
     3961 cagaaggatt tcccgtcatc atccaacgcg atattcaggc gtaaaccctc taaagccggg
     4021 tcgaaggcat agagctgcgc gcgaaatgtt tcaacattgc tcgctggtac gctgatttgc
     4081 gcgttaatgc cctcatgcgc cagatacact cgcccaaaaa cattcagcgc ggtaaacagc
     4141 tgatataaag cgtcacgggt cgccttagga tcggcgatgt ggaaatactt gtaaaacgaa
     4201 atggtggttc gcggttcgct ctcagccaac attttggctt ttaacgcgtc gttggaaatg
     4261 cggttgtgta acactggcat ggtgtacggt tcctgcgaga tgggaaagta aaaatccgcg
     4321 gcatgatata gcaattatcg ataattaaca tccacacatt ttacgctaca tttgcgcatt
     4381 aaaaattatt tgttatttac aagcgcggca atttcgccca gtcttcagtc acaaattttg
     4441 gttgcggacg aaaaaatgcg acaatacata caattgcccg tataggttga aaaacaggat
     4501 tgatatgacg aatctaccca agttctccac cgcactgctt catccgcgtt attggttaac
     4561 ctggttgggt attggcgtac tttggttggt cgtgcaattg ccctacccgg ttatctaccg
     4621 cctcggttgt ggattaggaa aactggcgtt acgttttatg aaacgacgcg caaaaattgt
     4681 gcatcgcaac ctggaactgt gcttcccgga aatgagcgaa caagaacgcc gtaaaatggt
     4741 ggtgaagaat ttcgaatccg ttggcatggg cctgatggaa accggcatgg cgtggttctg
     4801 gccggaccgc cgaatcgccc gctggacgga agtgatcggc atggaacaca ttcgtgacgt
     4861 gcaggcgcaa aaacgcggca tcctgttagt tggcatccat tttctgacac tggagctggg
     4921 cgcgcggcag tttggtatgc aggaaccggg gattggcgtt taccgcccga acgataatcc
     4981 actgattgac tggctacaaa cctggggccg tttgcgctca aataaatcga tgctcgaccg
     5041 caaagattta aaaggcatga ttaaagccct gaaaaaaggc gaagtggtct ggtacgcacc
     5101 ggatcatgat tacggcccgc gctcaagcgt tttcgtcccg ttgtttgccg ttgagcaggc
     5161 tgcgaccacg accggaacct ggatgctggc acggatgtcc ggcgcatgtc tggtgccctt
     5221 cgttccacgc cgtaagccag atggcaaagg gtatcagttg attatgctgc cgccagagtg
     5281 ttctccgccg ctggatgatg ccgaaactac cgccgcgtgg atgaacaaag ttgtcgaaaa
     5341 atgcatcatg atggcaccag agcagtatat gtggttacac cgccgcttta aaacacgccc
     5401 ggaaggcgtt ccttcacgct attaaatctc tcatgccgga tgcttcagaa tggcatccgg
     5461 cattaccaca gcaaatcccc ctgatttagc gataaaagct ctctggattg cgccccctgg
     5521 aagtcgggcg cataattagt gtgcttatct tttcttctta tgttcaccgc gcctggcgca
     5581 ccaacagcgg attgctatgt caccctgtga aaatgacacc cctataaact ggaaacgaaa
     5641 cctgatcgtc gcctggctag gctgttttct taccggggcc gccttcagtc tggtaatgcc
     5701 cttcttaccc ctctacgttg agcagcttgg cgttaccggc cactctgccc tgaatatgtg
     5761 gtccggtatt gtcttcagca ttacattttt attttcggcc atcgcctcac cgttttgggg
     5821 tggactcgcc gaccgtaaag gccgaaaact catgctatta cgctctgccc tcggcatggg
     5881 catcgtgatg gtgttgatgg gactggcaca aaatatctgg cagtttttga tcctgcgggc
     5941 gcttcttggg ttacttggcg gatttgtccc caacgctaat gctcttatcg ccacacaagt
     6001 accgcgtaat aaaagcggct gggcgctggg tacgctctcc acaggcggcg ttagcggcgc
     6061 gttgctcggc ccaatggctg gcggtttgct cgccgatagc tacggcttac gtccggtatt
     6121 ctttattacc gccagtgtgc tcatactctg ctttttcgtc accctgttct gcatcagaga
     6181 aaaattccag ccggtcagca aaaaagagat gctgcacatg cgggaagtgg tgacatcact
     6241 taaaaacccg aaactggtac tcagcctgtt tgtcactacg ttaatcatcc aggtggcgac
     6301 gggctcaatt gcccccattc tgacgctgta tgtccgcgaa ctggcgggta acgtcagtaa
     6361 cgtcgccttt atcagtggca tgatcgcctc ggtgccaggc gtggcggctc tgctgagtgc
     6421 accacgactc ggcaaacttg gcgatcgaat cggccccgaa aagatcctga ttacggcgct
     6481 gatcttctct gtactgctgt tgatcccaat gtcatacgtt cagacgccat tacaacttgg
     6541 gattttacgt tttttgctcg gtgccgccga tggtgcacta ctccccgccg tacagacact
     6601 gttggtttac aactcgagca accaaatcgc cggacgtatc ttcagctata accaatcgtt
     6661 tcgtgatatt ggcaacgtta ccggaccatt gatgggagca gcgatttcag cgaactacgg
     6721 tttcagagcg gtatttctcg tcaccgctgg cgtagtgtta ttcaacgcag tctattcatg
     6781 gaacagtcta cgtcgtcgtc gaatacccca ggtatcgaac tgatttttcg cctttcatac
     6841 ttgcaaaagc ggagaatcag ctatcctttt ccctgaaacc tcatcaactc aaagggagaa
     6901 tcgtgatgac catgtacgca acgcttgaag aagccattga cgctgcacgc gaagaatttc
     6961 ttgcagacaa ccccggcatc gacgccgaag acgcgaatgt gcaacagttc aatgcccaaa
     7021 aatacgtttt gcaggacggc gacatcatgt ggcaagttga gttttttgcc gacgaagggg
     7081 aagaaggtga atgtttacct atgcttagcg gtgaggccgc gcaaagtgtt tttgacggcg
     7141 actatgatga gatagagatc cgccaggagt ggcaagaaga gaatacatta catgaatggg
     7201 acgaggggga atttcagctt gagccaccgc tggataccga ggaaggacgc gcagcagctg
     7261 atgagtggga tgaacgttaa tcactcatac gggccatgat gaatgtcgat cggcagcagt
     7321 aaagtatcaa aaaccaggga gaatggcaga tcaagaattg tgacataacg ccaggcggag
     7381 tcacgcacat cccattgcac accgggataa tattggttgc catgcccctg ccccggtata
     7441 gtgcggctaa tgatgctgcc acagccactc agcaaggtca ccatgatgct caccacaatt
     7501 aaacgcattt gatacctctt ccgtaaaaaa ataccggcat aacgccggta tttttattga
     7561 ctcgttgttt ttgccagatg cgacgtgaac gccttaccca acctacaaaa tcttgcaaaa
     7621 tcaataaatt gcaggaacga tgtaggcctg ataagcgtag cgcatcaggc aactacgttt
     7681 ttattgcgaa gccgcatctg gtttacgcaa tgccagtgga ttgagcttta tcccttcgta
     7741 ataactcacc catgaagaat atctctcagg ggaattccag acgcggaaat gcagacgggc
     7801 catcgtcacc ggatcgctta gcagcaccag gcgacgatcg cgattcagct tctctggcgt
     7861 ctcgttcagc gcctgttcaa tgtggcggtc acgggcgatt tccagcacct tactggcgcg
     7921 gtgacgcgcg gtcgccattg cggtggccag agcgttaaat gacgggttaa atactgcgtg
     7981 cataaaacca tcatcaaggg agcgttgacg gttcatctca aggaaccgat cggtatcaac
     8041 cagcacctgc ggcggtgaat actcttccgg gatcaggaac agtttccagc gtttggtgcg
     8101 cagaccaacg gtggcacggc tggaaatcac cgaaacaaac ggtgacagga tcaacgagaa
     8161 gacaatcgga gccagccaga acaggaaacg cagatccagc cacgccatcc caacagccca
     8221 cactaaccct aacagcagct gtgagccgtg gcgtttgaac gcttcacccc aggaagtgga
     8281 gtcatcatca cgctgcggtg aattccacac cacttcccag ccaaggaacg cgctgacgac
     8341 gaagaccgta tggaacagca tgcgtaccgg agccagcagc acggaaaaca gcacttccag
     8401 cagcaacgat aatgtaacgc gccagaagcc gccgtattct ttcgttcctt tgcaccagat
     8461 aagcaaaatg ctcaatagct ttggcaggaa caacagcacc atggtcgaag caaaaagtgc
     8521 aatcgccagc tcaggacgcc actgcggcca caccgggaac aactgccgtg gttgcaggaa
     8581 gtattgcggt tcggttaacg catgcactac ctgcaatgca gtagagagcg cgaggaacat
     8641 aaaccacagc ggagcggaga gataagacat cacgcccgtc aggaataccg cacggtgaac
     8701 cgggtgcata cccttcacca ggaacagacg gaagttcatc aggttaccgt ggcaccagcg
     8761 gcggtcacgt tttagctcat caagtaagtt aggcggtaat tcttcataag aacccgggag
     8821 atcgtaagca atccagactc cccaacctgc acggcgcatc aacgccgctt ccacgaagtc
     8881 atgtgacagg attgaaccgg caaaggaacc ttcgcccggc agcggagcca gtgcgcagtg
     8941 ctcgataaac ggtttcacgc ggataatcgc gttatgtccc cagtagtgcg actcgccaag
     9001 ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac acgcgggtcg cgaactgctg
     9061 acagcgtgca tacagcgtat ccataccgga cgctttcggc gacgactgaa tgatcccggc
     9121 gttcgggttg gcttccatca agcgcaccag cccacacaaa caatcaccgg tcattaccga
     9181 gtcagcatcc agcaccacca tgtagctgta ctggctgccc cagcgacggc agaagtcatc
     9241 gatattaccg cttttacgct tcacgcgacg acggcggcgg cgatagaaaa tctgaccttc
     9301 gccaccgact tcagcgataa gctccatcca ggctttttgc tctgcgacgc agatatccgg
     9361 gttgtaactg tcactcagaa tgtagacatc aaagtgtttg gcattcccgg tggcttttac
     9421 tgattcccac gttgcacgca agccagcaaa aacacgattc acgtcttcgt tacagatagg
     9481 catgatcaac gccgtgcgat gctccgggtt taatggttca tcgccaactg ttgacgcaga
     9541 tatactgtat ttatcgcgac caataagcag ttgcaggaag cccatcaacg ccgtccagaa
     9601 tccggcggac acccaacaga acagtaccgc aaagaggatc aggataccgg tttgcagcat
     9661 ataaggcaga agctgcatga aggaaaccca caaatcctga ccaaccatat ccataggatt
     9721 aatcagcgcc caaccctgat aaggaagaat ggtcttcata taccaggtcg cgacgacagt
     9781 ttgcgcgagc gtcaggatca acagaatgta acgccggatg gtaccgacgg tacgccactt
     9841 ttgctcgctc tcctgctctt ctttggtcaa acgagccaga tagcgcggtg ttacatcgcg
     9901 tccacgcagg cgatcccaga aacggcctac cgggttggta cgccacgggt cgggaaacat
     9961 cgaggagcgt tttgcttctg gcatcgcctt aagctgatcg cgcccttcgt cgtctttaat
    10021 taactgtcca tcagcaagtg aatctggcca ggcttgttcc agacgcgcct ttaccgagcc
    10081 ttgcggggag tcatcctccc gcgcccaggt gcggtgttcg gcatccagcg cctgatgaac
    10141 ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt gcggcgatgg gcattgcgtc
    10201 aatgtactca gttgtcttat tcattggcag gtaactggta gctccaggtt tcactcaacg
    10261 tctgatcggc attcaccagc gcagcgcgca tttcagtggt tttcttggca tctttcactt
    10321 tcacacgcat caccagacgc cagcctttgg taaccgggtt atagcgcacc gtgctttcca
    10381 ctatctcacc attatcacca atgctggttt gcgctgtgac cggggtatct tctggcagtt
    10441 ttttcatctc tgcgccggta aaatcgacca caaaggcgat agtaccgtca ggctggcgaa
    10501 tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt ttgttgcacc catgcgttat
    10561 ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat ggtgtattta aagttcatct
    10621 ctttacccgg ctccggcagc tgatccggcg tccagtaagc gacgatgtta tcgttagttt
    10681 catcgttggt tggaatttcc accagctcaa cgctgccttt accccactcc cctttcggag
    10741 tcacccatgc gcttggacga agatcgtaac gatcatcgag atcttcaaag cgggagaaat
    10801 cacgaccgcg ctgcaacaga ccaaagccta gcgggttttc catggagaag ctgctgaccg
    10861 ctaaatgttt cgggttattc aacggacgcc agatccattc gccattaccg gcatggatag
    10921 agagaccgtt agagtcgtgc aactccggac gatagttatt tgcaggcgac ggttggttcg
    10981 gcccaaacag gaacatactg gttaacggtg caacccccag tttgccgact ttatcgcgca
    11041 gatagatttt cgactgcaca tccacaaccg tgtcacgccc tggcataact acgaatttgt
    11101 aagcacctgt cgcgcgcggc gagtcaagca atgcataaat ggttaaacgt ttatcagttg
    11161 gttttggacg ctcgatccag aactctttga agcgtggaaa ttcttcaccc gatggcaagg
    11221 cggtatcaat tgccagaccg cgggcagaaa ggccataaac ctgacctgca ccaatcacgc
    11281 ggaaatagct ggccccgagc atgctgacga tttcatcgtt tttatctttg ctgttgatcg
    11341 ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac cgtgtctttg tcatgctgaa
    11401 catcgccgaa agtgaaataa tccgggctgt atttgattcg tttgactgcg gtggcagtca
    11461 cttcatttat tttgaccggg gtatcgaagt acataccctg atggtagaac tcgagtttga
    11521 atggggtctt cagattgttc cagtacgctt tgtcatgatt aaactggatc tgctgatagt
    11581 ccgcgtattt catatcgcgg aaaacggagg gcaagttgct tttgggcgcc tcatagcctt
    11641 tcccggctaa ggattgagct tgctttgcga catcatcaat actgaaagcc cagcttgaag
    11701 atgtatacag ggttaacatt actgcagcac tcaaccaacg cattttcatc atttgtagtt
    11761 tatgtttcat aattagtaag cacttccccc tttgtgtgct tatatcgatc cgatctattt
    11821 taatggataa ccagccattc cgacaacaga atcccggtat cgttcagcac gctggctcat
    11881 ggattaagca cctgattgca accgttttca ctttccgtgc ttctcctaaa gacagaagca
    11941 tcgacttcgt gtagggtttg ccagcaaatt tatttatcaa ccgtcttaca gacgcttcca
    12001 ttctggttag tggtagcgat gatgctatgc ataaaaactt gaagcctgcg cctggcgcgg
    12061 tcaggtcggt gtcgctatgg caacaagcct aaaggcataa cccgacaggg ataagacgaa
    12121 aaatccgaga ttagttaacc atatatgaat ccagtacccg cgcaacgtga atatttcctc
    12181 gactccatcc gcgcctggct gatgttgtta gggatccctt ttcatatttc tttaatctat
    12241 tcgagccata catggcatgt gaatagcgcc gaaccgtcat tgtggctgac cctttttaat
    12301 gacttcatcc actcgttccg catgcaggta tttttcgtta tatccggcta cttttcctac
    12361 atgctttttt tacgctatcc cttgaaaaaa tggtggaaag tacgtgtcga acgtgtaggt
    12421 atcccgatgt taacagccat ccccctactg acattgccgc aatttattat gctgcaatac
    12481 gtcaaaggga aagcggaaag ttggcctggg ctgtcattgt atgacaaata taatacgttg
    12541 gcctgggaat taatatcgca cctgtggttt ttactggtgt tagtggtcat gacgacgctg
    12601 tgcgtatgga tatttaaacg catcagaaat aatttagaaa attctgataa aacgaataaa
    12661 aaattctcga tggtaaaact atcgctgatt tttttgtgcc tcggcatcgg ttatgcggta
    12721 ataagaagaa cgatttttat tgtgtatccg cccattctga gtaatggcat gttcaatttt
    12781 attgtcatgc aaacgctatt ttatttgccg ttctttatcc tcggcgcact ggctttcatt
    12841 ttccctcatc ttaaagcctt gtttaccacg ccgtctcgtg gctgtaccct cgctgcagca
    12901 ttggcgtttg tcgcttattt actcaaccag cgctatggca gtggcgatgc ctggatgtac
    12961 gaaaccgagt cggtgatcac catggtcctc ggtctgtgga tggtgaatgt ggtcttctcc
    13021 tttggccacc gtttgcttaa cttccagtca gcgcgggtga cttactttgt taacgcatcg
    13081 ctatttatct atctggttca ccacccgtta acgctgtttt tcggcgcgta cattacaccg
    13141 cacatcacct ccaactggct tggttttctc tgtggcctga tattcgtagt agggattgcg
    13201 ataattctgt atgaaattca tttacgcatc ccattactga agtttttgtt ctctggtaaa
    13261 ccggttgtta agcgtgagaa cgataaagca ccagcccgtt aagccgcgtt tacaataacc
    13321 attccacggg caatatcgac gccagtctga ccataacccg cttccagaaa ctggtggcgg
    13381 gttctttttt gagaacaatc tctttctttg catgacgatc aacccagttg atccgtcccc
    13441 acctgtccag tcggagctgc caggccgcat catactggct ctgaataaag cgtttatcaa
    13501 ttaactgagc cagcgtttcg ctctctatca cgaagcccat ttcagtatta agcaatgttg
    13561 aacgcggatc gaaattgaaa gaaccgataa acaccgtttt accatcgatg ctaaaggttt
    13621 tagcatgcag gctggcaccg gaattaccgg ttatgccgcg atcgtgtaac gtactacttt
    13681 gttcacgcgt cggcttgagt tcatataatt ccacgccata gcggagcaat tttttgcgcc
    13741 agcgcgcgta tccggcatgg acgacagcaa catcgttagc ggcaagagaa ttggttagga
    13801 tcgcaatctt tacccctttt ctcaccatcc gtaagagttg cgccacacct gctcgtgtcg
    13861 gtacaaaata ggaagagata atatcgatgc gttcactggg tgagcccatg atatcgaaca
    13921 ggcgctgcgg taacagtgaa tgccgttttg ccttgccctc ccctttcgcc ggatcatcac
    13981 ttaataaacg tgttttcgcc cagataagcg gcaacgttcc atcaaccaga tgatttataa
    14041 acggactgga ttccattttg cgtaagtaac gatgcgtcat ggcatcgtta tgccaggagg
    14101 cgggtaactc gatgcgatcc gccatttcac cttccgggac atccagcacc tgctgtaagg
    14161 gtgaaaccga tttgcaatac cagtagcggg cgaaatcatc ggcaacgtcc tctactacgg
    14221 gtcctattgc catgacatct aaatccgaaa aaagtggctc ttcccctgcc ccaaaatagg
    14281 catcaccaat atttcgcccc cctaccagcg tcaccacgcc atcgacagtg aaacttttat
    14341 tgtgcatacg gcgattgaga cgggaaaagt cggtgatata accaagcgga cgcagcaagc
    14401 gaaacgagaa aggattaaaa agccggactt caatgcgggg atgactgtca agcaagcgta
    14461 aaatgtcgtc aagtccgggc gtattgttgt cgtccagcaa caaacggacg cgaacgccac
    14521 gctttgctgc ggctaacagg gcggaaaaca gtaaccgacc cgacatatcg tcctgccaga
    14581 tgtaatactg aacatccagc gtatgctctg ctatttcggc cagacgatac cgggcggcaa
    14641 acgcatccag acttttctcc agagggaaaa gaccacactg accaggatgt tgcgaacaca
    14701 gtggcagcac cgcgctcgcc agccggggca aatcattcat ctccttgttg ggtaaggagt
    14761 ctttcgtaga ggtgggcgtt ttcttcatca taacagacaa agtatacctg ttcgggtaaa
    14821 gcgtgacggg taataaattc tgaaacggtt tttactgcga tttcagccgc tgccgcacga
    14881 gggtaaccat aaaccccagt actgattgca ggaaaagcca ctgacgtata gctgtttgcc
    14941 gccaccagtc gtaggctatt gagataggca tcctgcaaaa gctggtcttc gttttgttca
    15001 ccaccacgcc agactggccc gacggtgtgc actacggctt tagcaggaag atcgcctgca
    15061 agcgtaataa cggcatgccc cgtagggcaa tcgccctgct gttgcctgac ttttaaacag
    15121 gcatccagca gggccggacc cgctgcgcga tgaatggccc catcgacgcc gccacctccc
    15181 attaatgaag gattagccgc attcacaatc acatcaacgg ccagtttggt aatatcaccc
    15241 tgcacaacat gaatacgcgt tttcataacg cctccttaca ccttcttgtt tcttaagtgt
    15301 acagcaggct ttttggttaa atcgcatttt tctgctcagc aacgtgagtt gttcagaaac
    15361 ttagttatat tcgatgttca ttacagccag ctttttctgt ttatcaagat actgcacttt
    15421 tactgaggct atttgcgcgt ttttgacatt gccgctcatt aacgctttac tggagtaggt
    15481 tttatccgga acagaaccat tctgtgggca gttcatttct acgggtgatg actgggtgct
    15541 gacgtcacat gccggttcca caatttggcc ataaaaatgg atcacgcccc cttgcgcggt
    15601 gcttcctgcg atggcaataa aaggaaaaaa caaactgccg agcataagag agtttaaaaa
    15661 aggtctgaac atttattcta tttcctcaat gattagtcat ccttgagggt tgtgttatcc
    15721 atactttcag gttggctgat tcgcagcaaa ccattctctc cagattcatc ttatgctcga
    15781 tatttcaaca aattaagact tttctgaaga gggcggccat tgttgtgata aatgaagtga
    15841 ctgtccatca gaaacagtaa caactatttt cacccgatcg tccggggaaa tatttaaact
    15901 caacttcgtc aaagcaatgg gttgattagc aggcaatgaa agggtctttt cttgcttcgt
    15961 ctgactttgc cctgaactgc cttcgcgcag ggacaatatt tgtactctgc acagacaaga
    16021 ttgagtaaga gtgacttcag gaataatggt gtacatatcc ccttgctggg tcgtattaaa
    16081 ggttatctga ctggaaagtg ccgcaaggag taataacgca ttcatattct tctcccgaaa
    16141 aaaaacaggg cttgcgccct gtttctttaa tacagatgat gtattagtac tgatgagcgg
    16201 tcgcgttgtt accaaagcca acctgagtta cgttgacggt ggaattagat gcagtctggt
    16261 caaccgctgc accgttgccg ccaccgaact gtttaactgt catatgagag tctttaccgt
    16321 tccactgatc aagagtggca ctgttaccaa agccacgttg ggtcagatcg attgagctgt
    16381 catctgagcc ctgaccaaca tctgcaccgt taccaccacc atgctgggta atagtaagat
    16441 cagagttacg agcatcagct tgcagagcaa gtgcagagtt accaccaccg tactgataaa
    16501 tattcagctc tgaattcggg ccgctgttat taccgccacc accgtggtta ccgccaccgc
    16561 cgccgtactg aggaacaaca cctgccagag cgctaccgga gaatacgatt gctgcaattg
    16621 ctgctacttt taaaagtttc atgtaaaacc cccatcggat tgatttaaaa gtcgaatgga
    16681 aattaacgtt gtgtcacgcg aatagccatt tgcgactgtc tctgcactac aattgccgtt
    16741 ttttgagtac catactgtgt aatatttgct ttattaccag aacctttctg gataatcatc
    16801 gcagtattac cataagcacc ttgcgaaata ctggcatcgt tggcactgcc cgcctgatca
    16861 atatatgcaa ggttataatc tcctgtctgg tcaatctttg cccggttgct actaccttct
    16921 tgcgcaacaa ccgccaaaag ttttgagcct ccctgccgta actgagcact attattagtc
    16981 ccagcttgac caattatggc tgcctgatta aatgaagact tactcaattc atttaccgcg
    17041 aagttatatt ctgaattagc taaatcataa cctgctgcgg ctgcaatccc aggcgcaccc
    17101 agtattgtta acatcataaa taacaatt
//
LOCUS       DAZUCE010000101        17109 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZUCE010000101 DAZUCE010000000
VERSION     DAZUCE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.101"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            54..1193
                     /gene="arnB"
                     /locus_tag="ACHY03_002785"
     CDS             54..1193
                     /gene="arnB"
                     /locus_tag="ACHY03_002785"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFS3905994.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWSHVHAWHLFIIRVDEQRCGISRDVLMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1197..2165
                     /gene="arnC"
                     /locus_tag="ACHY03_002786"
     CDS             1197..2165
                     /gene="arnC"
                     /locus_tag="ACHY03_002786"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFS3905995.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREYGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2165..4147
                     /gene="arnA"
                     /locus_tag="ACHY03_002787"
     CDS             2165..4147
                     /gene="arnA"
                     /locus_tag="ACHY03_002787"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFS3905996.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAIN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4144..5034
                     /gene="arnD"
                     /locus_tag="ACHY03_002788"
     CDS             4144..5034
                     /gene="arnD"
                     /locus_tag="ACHY03_002788"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFS3905997.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGWDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5034..6686
                     /gene="arnT"
                     /locus_tag="ACHY03_002789"
     CDS             5034..6686
                     /gene="arnT"
                     /locus_tag="ACHY03_002789"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFS3905998.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6683..7018
                     /gene="arnE"
                     /locus_tag="ACHY03_002790"
     CDS             6683..7018
                     /gene="arnE"
                     /locus_tag="ACHY03_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFS3905999.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7018..7404
                     /gene="arnF"
                     /locus_tag="ACHY03_002791"
     CDS             7018..7404
                     /gene="arnF"
                     /locus_tag="ACHY03_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFS3906000.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7398..7664)
                     /gene="pmrD"
                     /locus_tag="ACHY03_002792"
     CDS             complement(7398..7664)
                     /gene="pmrD"
                     /locus_tag="ACHY03_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFS3906001.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7774..9129)
                     /gene="menE"
                     /locus_tag="ACHY03_002793"
     CDS             complement(7774..9129)
                     /gene="menE"
                     /locus_tag="ACHY03_002793"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFS3906002.1"
                     /translation="MIFSDWPWRHWRQVRGETIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPIELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9126..10088)
                     /gene="menC"
                     /locus_tag="ACHY03_002794"
     CDS             complement(9126..10088)
                     /gene="menC"
                     /locus_tag="ACHY03_002794"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFS3906003.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMHSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAVPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10088..10945)
                     /gene="menB"
                     /locus_tag="ACHY03_002795"
     CDS             complement(10088..10945)
                     /gene="menB"
                     /locus_tag="ACHY03_002795"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFS3906004.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10960..11718)
                     /gene="menH"
                     /locus_tag="ACHY03_002796"
     CDS             complement(10960..11718)
                     /gene="menH"
                     /locus_tag="ACHY03_002796"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFS3906005.1"
                     /translation="MILHAQAKHRKPGLPWLVFLHGFSGDCHEWQEVGEVFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11715..13385)
                     /gene="menD"
                     /locus_tag="ACHY03_002797"
     CDS             complement(11715..13385)
                     /gene="menD"
                     /locus_tag="ACHY03_002797"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFS3906006.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAADN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRVCDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNGTDGAQTLQQLLAQVSHL"
     gene            complement(13474..14769)
                     /gene="menF"
                     /locus_tag="ACHY03_002798"
     CDS             complement(13474..14769)
                     /gene="menF"
                     /locus_tag="ACHY03_002798"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFS3906007.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSITPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIAHHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14848..15153)
                     /gene="elaB"
                     /locus_tag="ACHY03_002799"
     CDS             complement(14848..15153)
                     /gene="elaB"
                     /locus_tag="ACHY03_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFS3906008.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15208..15669)
                     /locus_tag="ACHY03_002800"
     CDS             complement(15208..15669)
                     /locus_tag="ACHY03_002800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3906009.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            15734..16651
                     /gene="rbn"
                     /locus_tag="ACHY03_002801"
     CDS             15734..16651
                     /gene="rbn"
                     /locus_tag="ACHY03_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFS3906010.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16839..>17109
                     /locus_tag="ACHY03_002802"
     CDS             16839..>17109
                     /locus_tag="ACHY03_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFS3906011.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNDTLSIIRLIKNAC"
BASE COUNT         3978 a         4631 c         4369 g         4131 t
ORIGIN      
        1 actctttata gcaaatataa gtggacaggt attcaatggc ggaaggaaaa gcaatgtcag
       61 aatttttgcc tttttcgcga ccagcaatgg gcgtggagga actcgctgca gttaaagagg
      121 ttctcgaatc cggttggatc acaaccggtc cgaagaatca ggcgcttgaa caagcctttt
      181 gccagttgac gggaaatcag catgccatcg cggtcagttc agccaccgcc ggaatgcata
      241 tcacgctaat ggcgttggaa attggcaagg gcgatgaagt gattacgcct tccctgacct
      301 gggtttcaac cctcaatatg atttccttgt tgggtgcaac gccggtaatg gtggatgtcg
      361 accgcgatac gctgatggtc acgcctgaag ctatcgagtc agccattacg ccacgcacta
      421 aagccatcat tccggtgcat tatgccggtg cgccagcaga tattgacgcc attcgcgcca
      481 ttggcgaacg ttacggcatc gcagttatcg aagatgctgc ccatgccgtc ggtacgtatt
      541 acaaagggcg acatattggc gcaaaaggta ccgctatttt ttcatttcat gccattaaaa
      601 atattacctg tgcggaaggt ggcctgattg taactgataa tgaaaacctt gcccgccagc
      661 tacggatgct gaaatttcac ggtctgggtg tcgatgccta tgacagacaa acctggggcc
      721 gtgcaccgca ggctgaagtc ttaacaccgg gctataagta caatctgacc gatattaacg
      781 ccgcgattgc cctgacacag ttagctaaat tagagcacct caacacccgt cggcgcgaaa
      841 ttgcccagca atatcagcaa gcactggcag ctctcccctt tcagccatta agccttcccg
      901 cctggtcgca cgttcacgcc tggcatctgt ttattattcg tgtcgatgaa caacgttgtg
      961 gtatcagtcg cgatgtgttg atggaagcgt taaaagaaag aggtattggt accgggttac
     1021 atttccgcgc cgctcacaca caaaaatatt atcgcgagcg ttttcccacg ctgtcgttac
     1081 cgaataccga atggaatagc gaacgcatct gttcgttgcc gctgttcccg gatatgacta
     1141 ccgccgatgc cgaccgcgtc atcacagccc ttcagcaact cgcaggacaa taagccatgt
     1201 ttgaaatcca ccctgttaag aaagtctcgg tggttattcc cgtttataac gagcaggaaa
     1261 gcttaccgga attaatcagg cgcaccacca cagcctgtga atcactgggg aaagagtatg
     1321 agatcctgct gattgatgac ggcagtagcg ataattccgc gcatatgctg gtcgaagcct
     1381 cacaagcgga gaacagccat attgtgtcta ttttgcttaa ccgcaattac gggcagcatt
     1441 cagcgattat ggcgggattc agtcacgtta ctggcgactt aattattacc ctcgatgccg
     1501 atctccagaa tccgccagag gagatccccc gtctggtggc aaaagccgat gaaggttacg
     1561 acgtggtagg gactgtacgc cagaaccgcc aggacagctg gtttcgtaaa acagcttcga
     1621 agatgattaa ccggcttatt cagcgcacca ccggcaaagc gatgggtgac tacggttgta
     1681 tgttgcgcgc ctatcgccgt catattgtcg atgcgatgtt gcactgccat gaacgcagca
     1741 cctttatccc gattctggcg aatatcttcg cccgccgtgc cattgaaatt ccagtgcatc
     1801 atgccgaacg tgagtatggt gaatccaaat acagctttat gcgcctgatt aatttgatgt
     1861 acgacctggt gacctgcctt accacaacgc cgctacgtat gctgagtctg ctcggcagca
     1921 ttattgcgat tggaggtttt agcattgcgg tgctgctggt gattttacgc ctgaccttcg
     1981 gaccacaatg ggcggcagaa ggcgtcttta tgctatttgc cgtgctgttt acttttattg
     2041 gcgctcagtt tatcggcatg ggattactcg gtgaatatat cggcaggatc tacaccgatg
     2101 tccgcgcccg cccccgctat tttgttcagc aagttatccg tccatccagc aaggaaaatg
     2161 aataatgaaa accgtcgttt ttgcctacca cgatatggga tgcctcggta ttgaagccct
     2221 gctgtctgcc ggttacgaaa ttagcgccat ttttacccat actgataatc ccggtgaaaa
     2281 agccttttat ggttcggtgg ctcgtctggc ggcggaaaga ggcattccgg tttatgcgcc
     2341 ggataacgtt aatcatccgc tgtgggtgga acgcattgcc caactgtcgc cagatgtgat
     2401 tttctctttt tattatcgcc atcttatttg cgacgaaatt ttgcagctcg ctcccgcagg
     2461 tgcaattaat ctgcacggtt cgctgttacc aaaatatcgt ggtcgcgcgc cgctgaactg
     2521 ggtgctggtg aacggtgaaa cggaaacggg cgttacattg caccgaatgg taaaacgtgc
     2581 cgatgccggg gccattgtgg cgcaactgcg cgttgccatt gcgccagacg atatcgctat
     2641 tacgctgcat cataaattgt gccatgccgc gcgccagcta ctggaacaga cattacccgc
     2701 cattaaacac ggtaatattc tggaaatcgc ccagcgcgaa aacgaagcca cctgttttgg
     2761 tcgcagaacg ccggatgaca gtttcctcga atggcataag ccggcatccg tactgcacaa
     2821 catggtacgt gccgttgccg atccgtggcc gggtgccttc agctatgttg gcaatcagaa
     2881 attcaccgtc tggtcgtcgc gtgttcatcc tcatgccagc aaagcacagc cggggagcgt
     2941 gatttctgtt gcgccactgc tgattgcctg tggcgatggc gcgctggaaa tcgtcaccgg
     3001 acaggcgggc gacggcatta ctatgcaggg ctcgcaatta gcgcagacgc tgggcctggt
     3061 gcaaggttca cgcttgaata gccagcctgc ctgtgccgcc cgacgccgta cccgggtact
     3121 catcctcggg gtgaatggct ttattggcaa ccatctgaca gaacgcctgc tgcgcgaaga
     3181 tcattatgaa gtttacggtc tggatattgg cagcgatgcg ataagccgtt ttctgaatca
     3241 tccgcatttt cactttgtcg aaggcgatat cagtattcat tccgaatgga ttgagtatca
     3301 cgtcaaaaaa tgtgatgtcg tcttgccgct ggtggcgata gccacgccga ttgaatatac
     3361 ccgcaatccg ctgcgcgtat ttgaactcga ttttgaagag aatctgcgca ttatccgcta
     3421 ctgcgtgaag taccgtaagc gaatcatctt cccatcaact tcagaagttt atggaatgtg
     3481 tagcgataaa tacttcgatg aggaccattc taatttaatc gtcggcccgg tgaataaacc
     3541 acgctggatt tattcggtgt caaaacaatt acttgatcgg gtgatctggg cctatggcga
     3601 aaaagagggt ttacagttca ccctcttccg cccgtttaac tggatgggac cacgactgga
     3661 taaccttaat gcggcacgaa tcggcagctc ccgcgcgatt acgcaactca ttctcaatct
     3721 tgtagaaggt tcaccgatta agctgattga tggcggaaaa caaaaacgct gctttactga
     3781 tattcgcgat ggaatcgagg cgttataccg cattatcgaa aatgcgggaa atcgctgcga
     3841 cggcgaaatt atcaacattg gcaatcctga gaacgaagcg agcattgagg aactgggcga
     3901 gatgctgctg gcgagcttcg aaaaacatcc gctgcgccat catttcccac cgtttgcggg
     3961 ctttcgcgtt gtcgaaagta gcagctacta cggcaaagga tatcaggacg tagagcatcg
     4021 taaaccgagc atccgcaatg cccaccgctg cctggactgg gagccgaaaa ttgatatgca
     4081 ggaaaccatc gacgaaacgc tggatttctt cctgcgcacc gttgatctta cggataaacc
     4141 atcatgacca aagtaggctt acgcattgat gtcgatacct ttcgtggcac ccgtgaaggc
     4201 gtgccgcgtc tgctggaaat cttgagtaag cataatattc aggccagcat ttttttcagc
     4261 gtcggcccgg acaatatggg ccgccatctc tggcgactgg tgaagccaca gtttttgtgg
     4321 aagatgctgc gctcaaacgc ggcatcgctt tatggctggg atattttact ggcaggtacg
     4381 gcctggccag gtaaagagat tggtcatgcc aatgccgata tcattcgtga agcggctaaa
     4441 catcacgaag tcggcctgca cgcctgggat caccatgcct ggcaagcccg tagcggtaac
     4501 tgggatcggc aaacaatgat cgacgatatt gcacgcggcc ttcgcactct ggaagagatt
     4561 atcggtcaac cggtaacctg ttctgccgct gcgggctggc gtgccgacca gcaggtgatc
     4621 gaagcaaaag aagcgttcca tttgcgctac aacagcgatt gtcgtggggc catgccgttc
     4681 cgtccactgc tcgaatcagg aaaccctggc actgcgcaaa ttccggtgac attacccacc
     4741 tgggacgaag tgattggttg ggatgtgaaa gcagaagatt ttaatggttg gttactcaac
     4801 cgcatcctgc gagataaagg cacgccggtt tataccattc atgcagaagt tgaaggctgc
     4861 gcttatcagc ataattttgt ggatctcctc aaacgcgcag ctcaggaagg cgtgacattt
     4921 tgccctttaa gcgaactgtt atcagagacg ttgccgctcg gacaagttgt tcgcggaaat
     4981 attgccggac gtgaaggctg gctgggttgc caacaaattg cgggtagtcg ctgatgaaat
     5041 cggtacgtta ccttatcggc ctcttcgcgt ttattgcctg ctattacctg ttaccgatca
     5101 gcacgcgtct gctatggcaa cccgatgaaa cgcgttatgc ggaaatcagt cgggaaatgc
     5161 tggcatccgg cgactggatt gtgccccatc tgttagggct acgttatttt gaaaaaccca
     5221 ttgccggata ctggattaac agcattgggc aatggctatt tggcgcgaat aactttggtg
     5281 tgcgggcagg cgttatcttt gcgaccctgt taactgccgc gctggtgacc tggtttactc
     5341 tgcgcttatg gcgcgataaa cgtctggcat tactcgccac agtaatttat ctctcattgt
     5401 ttattgtcta tgccatcggc acttatgccg tgctcgatcc gtttattgca ttctggctgg
     5461 tggcgggaat gtgcagcttc tggctggcaa tgcaggcaca gacgtggaaa ggcaaaagcg
     5521 caggattttt actgctggga atcacctgcg gcatgggggt gatgaccaaa ggttttctcg
     5581 cccttgccgt gccggtatta agcgtgctgc catgggtagc gacgcaaaaa cgctggaaag
     5641 atctctttat ttacggctgg ctggcggtta tcagttgcgt actgacggtt ctcccctggg
     5701 gactgacgat agcgcagcgg gagcctgact tctggcacta ttttttctgg gttgagcata
     5761 ttcaacgctt tgcactggat gatgcccaac atagagctcc gttctggtac tacgtgccgg
     5821 tcatcattgc cggtagcctt ccgtggctgg gattactccc cggtgcactg tacacaggct
     5881 ggaaaaaccg caagcattcc gcaaccgtct atttgttgag ctggacgata atgccgctgc
     5941 tgtttttctc cgtcgctaaa ggcaaattgc ccacctatat tctttcctgc tttgcacctc
     6001 tggcaatgct gatggcgcat tacgctttgc tggcagcaaa aaataatcct ctggcgttgc
     6061 ggattaatgg ctggattaac atcgcttttg gcgtcactgg cattattgcc acatttgtgg
     6121 tctccccctg gggaccaatg aacacgccgg tgtggcaaac cttcgagagc tataaagtct
     6181 tttgtgcctg gtcgattttt tcgctatggg catttttcgg ctggtacacc ttaacaaacg
     6241 tcgaaaagac ctggtctttt gccgcgcttt gcccgctggg gctggcgttg ctggtaggat
     6301 tttcaattcc tgacagagtc atggaaggaa aacatccgca attttttgtc gagatgacac
     6361 aagaatcact tcagccaagc cgctatattc ttactgatag cgtcggtgtt gccgcaggtc
     6421 tggcatggag cctgcaacgc gatgacatca tcatgtatcg ccagacaggt gagttgaaat
     6481 acggccttaa ttatccggat gcgaaaggga gatttgtcag cggtgatgag ttcgcaaact
     6541 ggcttaatca acatcgtcag gaggggatta ttacactcgt gctttcggtt gaccgcgatg
     6601 aagatatcaa cagtctcgcc attccgcccg cagatgccat cgatcgtcag gagcgtctgg
     6661 tgctgattca gtatcgtccc aaatgatctg gctaacatta gtctttgcca gcttgcttag
     6721 cgttgccggg cagttgtgtc agaaacaggc aacctgcttt gtggcgataa acaaacggcg
     6781 caaacatatc gtgctgtggc tgggactggc gctggcttgt cttggtcttg ccatggtgct
     6841 ctggctgctg gtcttgcaga acgtaccggt aggcattgcc tacccgatgt taagtctgaa
     6901 ttttgtctgg gtgacgctgg ctgcagtaaa actgtggcac gaaccggtat cgccgcgtca
     6961 ctggtgtggg gtggcgttca ttattggtgg cattgtgatc ctcgggagta cggtgtaatg
     7021 ggcctgatgt ggggattatt cagcgtcatt attgcctcag ttgcgcaatt aagcctgggt
     7081 tttgcggcga gtcatctgcc gccgatgacg cacttatggg attttatcgc ggctctgtta
     7141 gcttttgggc tggatgcccg gatactgtta ctcgggctgc tgggttatct gctttccgtt
     7201 ttttgttggt ataaaacgct acataaactt gcgctaagca aagcctatgc cttgctcagt
     7261 atgagttatg tgctggtatg gattgcttca atggtacttc ccggctggga agggactttt
     7321 tcgctgaaag cactactggg agtagcctgt attatgagcg ggttgatgct gattttcctg
     7381 cccacgacaa aacaacgtta ctgagttttc cctgccactt tacactgccg ttcccactca
     7441 tcggggctat aactgcttgc cgagaggact ttcaccgtat gcagcttttc acgattaata
     7501 caataaagcg cattctgcaa aggcgagagt aaatccccca ctttcacggc gaagtcactc
     7561 ttcacttcgg cgatcatttt tatcgcgccg ccagcatcgc agagcataag tacatgtctg
     7621 ttatcttgtt tattgcagca cgattttttg accagccatt ccattgcatt atcctgtttg
     7681 ctaagagttt tcacatcaat ttacagatta acagataaac tctttatcaa cggcaatgga
     7741 atggctgaaa tgagaagtga aacggagggt taattattgc tgacgttgca cccactcttt
     7801 tagcgcctga cgtgaaattt taataccgcc gtttttcagt tcaggcggca acgttaacca
     7861 acgtaccggt tgctgaaaac gggccagctt atctttcacc cattcactaa gatcaacgct
     7921 ctcgtggtca tactccatca ccgccaccgg tcgatgacca aactccttgt cggcaacggg
     7981 gacgataaac acctgcaaaa ccgcaggatg cgcagcaatt acgcgctcga cttcttccgg
     8041 ctgaataccc tctccgccac tgaagaatag attgtctaaa cgtccgacaa tggtcagctt
     8101 gccattatgc atctcaccgc gatcgcgcgt agcgtaccag ccttcgtcat taaccagtga
     8161 aaccagttgc ccgttacgcc agtaaccttc tgccatactg gcagcccgca gccacacttc
     8221 attattaacg attttcactt cccgacccgg cagcggcgaa ccaacgtctg ccaggccgtc
     8281 ggcttctttc gcacacaccg tggaggcaaa ctcggtcaga ccatagccgc aaaagcaacg
     8341 aatcccctgc tcgcgcgcct gttccgtcaa ctcgatcggg atagccgcgc cgccaagtaa
     8401 caccgctttc agggaaacgg aactgcggtt aaccagcaaa cgccagagtt gtgttggcac
     8461 cagtgaagcg tgagtacagc ctgccagcat ttgctccaat ggctgtttat cacgcaccgt
     8521 catccgcgca ccagcgtata accagcgcca cataattccc tgaccggaga cgtgaaacag
     8581 cggtaaagag agcaaccagt catcgtgatc gccaaacgga atcagcgata acaccccttc
     8641 ggcactggca agatgggcct gataagtatg gacagcggct ttcggcaacc cggtagaacc
     8701 cgaggtcaac gtcattgagc acagacgcgt cggctgccac gcagcggcat gtgcgccttc
     8761 aacccgctga atgtgcagcg acgttaatgc cggaaacgtg ttttccccat ccggcaccag
     8821 cgcaaattgc agcgtcagat tgggcagcaa ttcttcaagc aacggttgcg gcagctgagg
     8881 gttcacgggc aacacccgcg ccccgcattg cagtaacgcc agccaggcga gcagcgtttg
     8941 cggcgtattc cacgcccgca acatcacgcc gctgccctca acaacgccct gcgccgcaaa
     9001 gccggaggct aattcatcga cgcgagcaca aagctcgcgc cagttgagtt gctcgtcatt
     9061 aagacgtaag gcgatggttt ctccccgcac ttgccgccag tgacgccacg gccagtcaga
     9121 gaagatcata acaaccgctc cagtgcatca acttccacga caggcagcgt gctacccggc
     9181 cagcgacgta cctgctgcgc ctgcattaga tccagcgtgt ccagccctgg aatggtgtcc
     9241 ggcgttaacc aggcggcaat ccgcgccagt tgcgttaagc ctaagctcga ttcaatggaa
     9301 gaactgatca ccgccgtcag ccccagcgcg tgcgccgcct gtacctgctc gcgtactttc
     9361 tccagactgc ccgtgagcgt gggtttgata actaccgcgc gcacgccctc ttcagccaca
     9421 aaggcaaaat ccggctcgcg caagctttca tcccaggcaa tggcaatgcc ggtttcacgg
     9481 gcaaacgctc gcgaatcatc gcgggttttg cacggctctt cgagaaaagc aatgcggtgg
     9541 cgataatccg ggttaacgta tttggcaaac tgctggcctt tcagcggcgt ccaggcgcga
     9601 ttcgcgtcaa gacgcaaatg cagatcagga accgcctcca gcaacagatt gaccaccatg
     9661 ccgtcgcgca cggcttcgta caatcccact ttgaccttcg ccactttctc gcccggcata
     9721 tcggcaagtt tgaggatcag atcgtccgga tcgccattac acagcggtgc cgcacggtag
     9781 ttggctgctt gcggcaacgt atctgtcagt tctgccaatg cacagcttac gccaaaggcc
     9841 acggaatgca tctgcggtag ctcgcaatcg cctgccagcc agttatttac ccaggcaagc
     9901 agcacgcttt gcgcctcttc ccaggtttcc tggctgaagc ccggcagtgg ggagatctcc
     9961 ccccaccctt cgcgctcgcc ctcacgcagg caaacgtaca gcccgtcgcg ggtttttaac
    10021 cgcctgtcgc gcagaaccac ccccgcgtcc atggggatct gccagcggta tacctgcgcg
    10081 ctacgcatta cggattccgt ttaaatttgc tgaaatcagg ctgacgtttc tggttgaaag
    10141 cgttgcgacc ttcctgacct tcttccgtca tgtagaacag catggtggcg ttgcccgcca
    10201 gctcctgcag ccccgcctgc ccgtcacagt cggcgttcag cgcagctttc aggcagcgca
    10261 gcgccatcgg gctgttttgc agcatttcgc ggcaccaacg gacggtttct ttttccagat
    10321 ccgccagcgg taccacggtg ttcacaaggc ccatatccag cgcctgtttt gcgtcgtact
    10381 gacggcacag gaaccagatt tcacgcgctt ttttctgccc gacgatgcga gccatgtagg
    10441 aagcgcccca gccgccgtcg aaggaaccga ctttcgggcc agtctgaccg aagatggcat
    10501 tatctgccgc gatagtcagg tcgcacatca tgtgcagaac gtgaccaccg ccgatggagt
    10561 agccagccac catcgcgaca accggtttcg gacaggtacg gatctgacgc tggaagtcca
    10621 gcacgttcag gtgatgtacg ccggaatcat ctttatagcc gccgtaatcg ccacgcactt
    10681 tctggtcacc accggagcag aacgctttat cgcctgcacc agtcagaatg atcacgccga
    10741 tgttgtcgtc ataacgtgca tctgccagtg cctggatcat ctcttttacc gtcagaggac
    10801 ggaaggcatt gcgcacctgc ggacgattaa tggtgatttt tgcgataccg tcggtggatt
    10861 tttcataacg aatgtcctcg aaaccttcgg agcagtcgtg ccattcaacc ggtgcgtaaa
    10921 gcattgcttc atcaggataa atcatattgt gtcctttagt cagaaacgca agatctgcgc
    10981 cagacttgcg attacgccag cgggattttc ccggtgcgcg ttatgtccgg cgcgaggaat
    11041 gacatggcag tcggcagcca gttccgccgc cagagcgcgg aatttgctgt cacgttcacc
    11101 acataaataa taaaacgcaa atgtgcgggc gctaaggtta gcacgtaaat caggctggac
    11161 ggcgagagaa gtcgcctcca gcatggcggc aagggttgcg ccattattgt tgctgcgcag
    11221 cgccaccagc tcccggcgtt gatcgtcatt gagcgaggca aaaacaggct gttgatacca
    11281 gtcggcaaat accgccgtta acggttctgt gcgaaaacgc tgcgcccatt ggcgatcgga
    11341 acgctgacgt tccgcacgtt gttcagcatt ttgcagcccc ggatgcccgc cttcgacaat
    11401 aaccccacaa agccccgcca gcccctggca agccgccatc atcgccaccc gtccgccaag
    11461 cgagtacccc accagccaga agttaaggat gttgtaacta accaaggttt tacgcagtaa
    11521 gtcggtgaca tcatcaaatc catcgacgct aatcgccgcc gaaccaccgt gacctgggag
    11581 atcaacatac aaccgtgagt agtcggcaaa cacctcgccc acttcttgcc attcgtggca
    11641 atcgccggaa aaaccgtgca aaaaaaccag ccagggtaaa cctggtttgc ggtgttttgc
    11701 ctgcgcgtgc aggatcataa atggcttacc tgcgccagaa gttgctggag cgtttgcgca
    11761 ccatcggtgc cgttaaccac catttcaatc accgtggtgg ttggcgtgcg ccaggcgtca
    11821 gcaagcgccg tttcaagttc ctgccagttt tgcggacgat gatatttcag ctcgaacatc
    11881 gcggcggcgt gctcaaaatg gacgttttgc ggcatcagat agaaacgctc gcgctcgctt
    11941 ttcggcgttg gcagcagcga gaaaatttgc ccgccgttgt tgttcaccac aattaatacc
    12001 agcggcgcgg atacctggcg caataacgcc agcgcattca ggtcgtaaag tgcggagaga
    12061 tcgcccacaa tcgccagcgt cggtttgccg cttgcccgct gaacgccggc ggcagtcgag
    12121 agcagcccgt cgataccgct ggccccacgg ttgctgtaca ccgggtaacc tgccggaagt
    12181 tgcgaaagcg catcaatcag acgtaccacc aggctgttac cgacaaacaa ttgcccctgt
    12241 tcaggcagat agtcgcagac gcgatgcgcc agttgcgctt cgccaaacgc atcgcgacgg
    12301 gcaataaccg cctgcatcgc ctgctccgcc aggcgcggga tttcaacgca ccagggctgg
    12361 cgtttttctg ccggatgcag ttccagccag tcggcaatat tggcaattaa ccgacgcccg
    12421 cgatggtgcg ccggatcaag ccgcccttcg atgtcatcca caatccagta ctcttcgggt
    12481 tcacagcttg cctgccattg caggagccgt ttgccagtca ggctgcttcc cagttgcacc
    12541 acaatttgcg cctgttgcag ttcgctggtc gctttggcat tgcctaacca gagatcggca
    12601 cacggcagcg gctgtccggt ttgcgacagc acgtcgccaa tcagcggcca gccaagagtt
    12661 tgcgcccaca gtgcaacttt tttgccctct tccgcactca tgcgcccggc aaccaccacg
    12721 ccgcgctttt gccgccagaa gaaccagtcg cgctgttttt cgctctcaag acgaggcgct
    12781 tcacgcagcc acggcttatc atcctgccac cagtcaccca gccgctgttg ccagctaagc
    12841 ccggtgtcgt ccatctcgcc atacagcggt tcagcaaacg ggcagttgat atggacccca
    12901 ccagcatgaa gcgtaccgag agcatggtcg atggtagaaa ccagccaacg tgcggggata
    12961 tcctgggtcg ggcgcggcag tgaaatactg tgcgtggggt gagaggcgaa cattcccggc
    13021 tgacgaatcg cctgattcgc gccgcagtca attagctccg gcgggcgatc ggcggttaag
    13081 agaatcagtt tttctccggt taacccggct tcaatcagtg ccggatagag atttgccacc
    13141 gccgtaccgg aggtgacaat caccgccacc ggctgcttgc tgactttcgc cagccccagc
    13201 gccagatgcc ccagtccacg ctcatcgaaa tgggtgtggt gaatgaatgc ggaattatcc
    13261 gccgccgcta acgttaacgg tgtagaacgc gagccagggg cgatacagat gtgtcttacg
    13321 ccgtgacgcg ttaatgcttc cagaatgacc gccgcccagc gtcggttaaa tgcgcttact
    13381 gacatgagtt tgtccggtat caatattgcg gctaagtata aggagctaca aaaatagatt
    13441 attgatatga atcggtaatg atgcgactca ttactattcc atttgtaata aagtacgcag
    13501 ccctgccgct ttgttgtcga tttcctgcca ctcttgctcg gggtcggaac cacggacaat
    13561 gcccgcgcca gcatataatc gcacgacatt gccgctgatt tttgctgagc gcagggaaac
    13621 gcagaattcg ctttgttgta atgagagata gcccgcagaa ccagcgtacc attctcgggt
    13681 gaacggttcg tgatgggcga taaactgtcg cgccagatcg cgcggtaaac cagcaactgc
    13741 tgccgttggc tgcaactgat gtaaacagat cacatcatcc gctttgttga gtgaagtcca
    13801 gatacagcgg cgaagatgct gcactttacg cagacgcagt acctgcggcg gtaaaacatc
    13861 cagcgtctgg gtatcggcct gtaatcgttg gcagatatct tccaccacca acatattctc
    13921 acgctgattt ttatcatccg ccatcagcca ctcacctaac tgctgcgcct gcttatcatc
    13981 aggatgattt gctactgttc ccgccagcgc ttcagtacgc agcgctttgt cacgccgccg
    14041 ccataaccgt tccggtgaag agccaagaaa agcattttcg ccatcaaagg ccatgtaaaa
    14101 atggtagcaa ttcagattca gtcgacgact ggccgccatc atcgccgccg cattgaccgg
    14161 acttgcgaaa tgcaggtcag ttgcccgagc aagcaccact ttgtcgagct caccttcggc
    14221 gatggttttc gttgccagtt cgattaattg cgtccagccc gttttgtccg gccagtgttg
    14281 ttctcgcgta gtggttaaat gtaacccagg caagggcgtg atactcacca gtgtggcgat
    14341 aaattctctt gccttaatcg catcgtgctg aagggagctt tcgctgaata gcgtcagccg
    14401 cagcgtggct ttaccgccac agcgtcgcca ttccaggcgt ggtaaaagta aattgccctg
    14461 cgacgggtca aacgcattca gcccccaaat gcgtaagtcg gcgtgttccg ggtgctggcg
    14521 aagaaaacgt tgtgcctggt ccaacgacgt aaaacgggta atcgcgccca ggacggcagc
    14581 ttcttcatca ccattacgtt gttgccagta gaattgcggg tacgtttgct gactggccag
    14641 ccagctcaag gcatcaaaag cgtctttgag agggaaagga atatcgataa cccgaatgcc
    14701 gggtgtcgcc ggaatctctt gcgacaaatg gcgcagtaga ttttccagcg ccgtagtaag
    14761 tgattgcacg cggacctctc cctgttaaaa gcctcacatt atacggggta ctacaaaaaa
    14821 aatgcagtac cccggtgtag ggaggtttta acggcgtgcc agcaacagtc ctagtaccag
    14881 cccaacggcc gcacccacac caattccctg ccagggtttt tcgtggacgt agtcatcagc
    14941 acgataaaca gcctgcttcg cccgatagta ataactgtct gaagcctggc taacccgttt
    15001 tttcacatca tccagcgctt tttctgcacg cgctttcagc tcaacatatt tctgatcggc
    15061 gggatcgcca gaggagcgga gcacctcttc cagtgtttca ctaagcagcg tcaggtcgtc
    15121 atcgatacgt gtatcaccaa actgattaga cattctcgtt ctccatttgc gtaaaacctg
    15181 tcactaacta tagacaaggg ttattgatta cgcctgaatt acctcgcgtg ccatcccaat
    15241 gtgtgggata ccatcttctt catagatctc cgtcacaggg ataaaaccga aactctggta
    15301 gaagttttgc agatgcgcct gcgccccgag gtagacaggc ttatcaggcc agtgatgcgt
    15361 gcaagtgtcg agggttttac tcatcagttg ctgccccact ttttcgccac gcaacgcttc
    15421 actgacaatc acccgaccaa taacgaccgg ctcaagatca tcatcacttt tcagaatcct
    15481 cgcatatgcc accagttcat catttttcca accgagaata tggcgattat cccccgtcaa
    15541 atcatcacca tcgatatcct gataagggca attctgctcg acaacaaaca ccgcgcaacg
    15601 cagttgtaat aaggcataaa gctgggaaac cgataattca gagtgatgca gatcttgcca
    15661 ttcaatcata atgctctcct tttagatgta tgccttatac tagccggaat attttttgaa
    15721 acgtgatgaa ctcatggaat taattttttt aggtacttcg gccggtgtgc caacccgcac
    15781 gcgcaatgtc acggcaatat tgcttaacct gcaacatccg acccagagcg gactttggtt
    15841 gtttgactgc ggtgaaggca cccagcatca gctactgcat accgccttta accctggaaa
    15901 actggacaag atttttatca gtcaccttca tggcgatcat ctttttggtt tacccggctt
    15961 gctgtgcagt cgttctatgt caggcattat ccaaccctta acgatttatg gtccgcaagg
    16021 tatccgtgaa tttgtggaga ccgcgctgcg gattagcggc tcatggaccg attatccgct
    16081 ggaaattgtc gaaattggcg ctggcgaaat tctcgacgat ggcctgcgca aagtgaccgc
    16141 ttatccaatg gaacacccac tggaatgtta tggctatcgt attgaagaac atgataaacc
    16201 gggtgcatta aatgcccagg cattaaaagc tgctggcgtg ccgcctggcc cactgtttca
    16261 ggaattaaaa gcgggcaaaa caatcacgct ggaagatgga aggcagatta acggcgcaga
    16321 ttacttagct gctccagtgc caggtaaagc gctcgctatt ttcggcgata ccggcccctg
    16381 cgatgccgca cttgacctgg ctaaaggtgt cgatgtcatg gtgcacgaag cgacgctgga
    16441 tataaccatg gaagccaaag ccaatagtcg cggtcatagc tctacacgcc aggctgcgac
    16501 actggcccgt gaggctggag tcggtaagct aatcattacc cacgtcagct cgcgctatga
    16561 tgacaaaggt tgtcagcacc tgttacgtga atgcaggtca attttcccgg cgactgaact
    16621 agcgaatgat ttcaccgtgt ttaacgttta actctgctta ttattaaaaa cagggcgaaa
    16681 cttgccctgt tatcgcaacc cgcgctacat atatccactc acacgggcat attgagcgat
    16741 ttttgatatt tattcatctc cttacgcact atattcagag caataagttg cctcttttta
    16801 tcttcattca gcacaacctt tgcaactaaa aggagcatat gatggttaca gttgtcagca
    16861 attattgtca attatctcaa acccaactca gtcagacatt tgcagaaaaa tttactgtga
    16921 ccgaggaatt actgcagtct ttaaaaaaaa cagcgttatc cggagatgaa gaaagtattg
    16981 agttactgca taatattgcg ttaggttatg atgaatttgg aaaaaaagct gaagatattc
    17041 tttaccatat tgttagaaac ccaacaaatg ataccctatc gattatccga cttattaaaa
    17101 atgcctgtt
//
LOCUS       DAZUCE010000102        17104 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZUCE010000102 DAZUCE010000000
VERSION     DAZUCE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.102"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..299
                     /locus_tag="ACHY03_002803"
     CDS             <1..299
                     /locus_tag="ACHY03_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3906012.1"
                     /translation="FRASVSYICHEFTKEYRISPINYVIQRRMTEAKWLLTNTELSQA
                     EISWRVGYENVDHFGKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(342..1493)
                     /locus_tag="ACHY03_002804"
     CDS             complement(342..1493)
                     /locus_tag="ACHY03_002804"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFS3906013.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1507..3102)
                     /locus_tag="ACHY03_002805"
     CDS             complement(1507..3102)
                     /locus_tag="ACHY03_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFS3906014.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3247..4005)
                     /gene="aroD"
                     /locus_tag="ACHY03_002806"
     CDS             complement(3247..4005)
                     /gene="aroD"
                     /locus_tag="ACHY03_002806"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFS3906015.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4036..4902)
                     /gene="ydiB"
                     /locus_tag="ACHY03_002807"
     CDS             complement(4036..4902)
                     /gene="ydiB"
                     /locus_tag="ACHY03_002807"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFS3906016.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4914..6179)
                     /locus_tag="ACHY03_002808"
     CDS             complement(4914..6179)
                     /locus_tag="ACHY03_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3906017.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6406..7620)
                     /locus_tag="ACHY03_002809"
     CDS             complement(6406..7620)
                     /locus_tag="ACHY03_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3906018.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7720..8076)
                     /locus_tag="ACHY03_002810"
     CDS             complement(7720..8076)
                     /locus_tag="ACHY03_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFS3906019.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8212..8319)
                     /gene="rprA"
                     /locus_tag="ACHY03_002811"
     ncRNA           complement(8212..8319)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHY03_002811"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8505..9617)
                     /gene="ydiK"
                     /locus_tag="ACHY03_002812"
     CDS             complement(8505..9617)
                     /gene="ydiK"
                     /locus_tag="ACHY03_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFS3906020.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10006..13062
                     /locus_tag="ACHY03_002813"
     CDS             10006..13062
                     /locus_tag="ACHY03_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFS3906021.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13059..13469
                     /gene="menI"
                     /locus_tag="ACHY03_002814"
     CDS             13059..13469
                     /gene="menI"
                     /locus_tag="ACHY03_002814"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFS3906022.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13569..13757
                     /locus_tag="ACHY03_002815"
     CDS             13569..13757
                     /locus_tag="ACHY03_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFS3906023.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14306..14674
                     /gene="sufA"
                     /locus_tag="ACHY03_002816"
     CDS             14306..14674
                     /gene="sufA"
                     /locus_tag="ACHY03_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFS3906024.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14683..16170
                     /gene="sufB"
                     /locus_tag="ACHY03_002817"
     CDS             14683..16170
                     /gene="sufB"
                     /locus_tag="ACHY03_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFS3906025.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSDVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16180..16926
                     /gene="sufC"
                     /locus_tag="ACHY03_002818"
     CDS             16180..16926
                     /gene="sufC"
                     /locus_tag="ACHY03_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFS3906026.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLHALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPGLCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16901..>17104
                     /locus_tag="ACHY03_002819"
     CDS             16901..>17104
                     /locus_tag="ACHY03_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310415.1"
                     /note="with SufBC activates cysteine desulfurase SufS;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFS3906027.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNIQFVSIVGE"
BASE COUNT         4494 a         4166 c         4239 g         4205 t
ORIGIN      
        1 aatttcgtgc cagcgtcagt tatatttgcc atgaatttac caaagagtat cgtatttccc
       61 caattaacta tgttattcaa cggcgtatga cggaagcgaa atggttactc actaatactg
      121 aattatcaca ggcagagatc tcctggcgtg tgggttatga aaatgtcgat cactttggca
      181 aactgttttt gcgccatgtc ggctgttcac ccagcgatta ccgcaggcaa tttaaaaact
      241 gttttgcgga acaagaaatc ctatctgaat ttcctcaacc ggtaagtctt gttggataaa
      301 tctggcggcg cagtttgtta agaagctgcg ccgcctgcag attatttgtt ctgatagtct
      361 ttcaggatct gccgacctgc tacgtaaatc ataatttcgt ctgtaccgcc gccgatacgt
      421 tcacaacgga catcacgcca gaagcgggag acgcgcgctt catcggtata gcccagaccg
      481 cccatgattt gaatcgcatc atcaatgact tccattgcgg tacgtgcgca atacagtttt
      541 gccagcgccg cgctggtgcg cagtgactga tgctgatcgg cttgccatgc cactttcagc
      601 accatgttgc gcatgttgtc gatcttaatc gccatcagcg ccagtttttc ctggatcatc
      661 tggttatgcc caatgggttt accaaaagcg atacgctggt tggcatagcg agcggcatct
      721 tcaaaggcac actcggcaaa gccggtgctg cgcgcggcgt tgatcaggcg ctccatctca
      781 aagttgtaca tcacattgag gaaacccatt ccttcttcgc ccaccatgtc gctctcttca
      841 acttccacgt tgtcgagata gacttcgcag gtgctgagca tatgccagcc gattttatgc
      901 agcgggttaa tcttaatgcc gggcttactg gagtcgaccc accacagggt gaaggctttt
      961 ttgggatctt tcggttgcgg atcgcgcgcc aacaccagca tatacgggta ctctttcgca
     1021 ccggtaataa aggttttctg tccgttgatg taaaccttgc cgtttttacg cgtgtaagtg
     1081 gtagtggcac tgttgttgtc tgagcctgcg cctggctccg tcaacgccag ggcataggcg
     1141 ggatcacccg tttccagggt gctttctgcc gttttacgta gctgctctgc agaaccgaaa
     1201 cggcgcatac tgtgaataca ttgaccgttg gtgatcaaaa atgccggagc accgcatttt
     1261 gacacttcca tcagcgccag catctgggtg acgtaatccg cagggatacc accaaattct
     1321 tccggcacgc caagcatgga aataccgtta tccgccagtg cccgcataaa ttcacgcggg
     1381 tatgtcccgt tttgatcgca ggtgcggaaa tactcttccg gaaagttagt cgtaatcagt
     1441 tcgcgaatac tggccagcag cagttcttgt tcttcagtta aagaaaaatc catcgtgata
     1501 ctcctgttaa tgagccgctt caggcaggac aaaacccatc gccgcatcga taaataatct
     1561 ttcgtccatc agtttgagtt ctggcgaaat cactggggtg aaatccattt tgtcgagaat
     1621 atctttttgt aaatcgacgc caggggcgat ttcgattaaa tgcaggccgt cttctttcag
     1681 cgtgaatact gcgcgctcag tgatataacg aacatccagc cctcgctcga gagcgatttt
     1741 tccgctgaaa gtaatttccg gtagttcccg aataaatttc ttcacccgtc cttcctggac
     1801 gatatttaat ttgccgtcgg taatttctgt ttttaaactg cccgcagtta atgtgccgca
     1861 gaaaatgatt ttcttcgaag tggcactgat atcaataaat ccaccggtgc ccatgatttt
     1921 accattgaat ttatgcacgc cgacattacc gtgctggtcg acttcagcaa aactcaaata
     1981 acaaacatcc agaccaccac cgtgataaaa atcaaactgg gacgtcatat ccagaatggc
     2041 acgggtattc acgttcgcgc caaaggcgat accctgtgaa gtaattccgc caatcggacc
     2101 tgtttctacc gtcagaataa agtcatcagc acaaccttct tcccgggcga ccaggccaat
     2161 gccgtcagca ataccgacgc cgacattccc caccgcgcct ttacgcattt cgaataatgc
     2221 acgccgcgca actaatttac gttggtttag gggcaggcta agtttagtgc tgtcatcaag
     2281 ggtgaagtca ccagaaataa agcggttaac cggcgcaccg ccatacagtt gggtttgatc
     2341 cggatcgacc accacaatat ccaccagata acccggaata cggacagatt taggatgcag
     2401 cgtggctttc ttaaccattt tctgcacctg catcatcaca ataccgccgt tattgtgcac
     2461 cgcctgggca ataaccaatg cgtcgagata catcacttca tcttcaaaag tggcgtagcc
     2521 ttcactgtcg caggtggtag cgcgaatgaa ggcgatatct ggcgcaatcg ctttgtaata
     2581 gagatattct ttgttatcaa actcgaccag tttaatcagg tcttctttag tgacttcatt
     2641 cagtttgccg ccttgctggc gtggatcgac aaatgtcccg atgccaatat cactaataat
     2701 accaggctgg tgggctgcgg cggcgcgtaa ggtttgtgta agtacacctt gtgggtagtt
     2761 ataagcaata attttatttt gttctgcgag ttcagaaata cgcggcgatt gtccccagtg
     2821 accacataat gcccatttca ccagaccttc ttgcgccagc ggactaatac cgcggtcggc
     2881 gcgatcgcca agcccggttg gactaataat cgataaatta cgtggcgttt gagtctgttt
     2941 atatttatca gcaagagcag taattaacgt ggtggcttcc agaataccgc cgccagcgcc
     3001 taacacacaa agtgttgctt cgtcgggaat ataattcacc gcttcctgtg ccgacaggac
     3061 cggcactcgt ccattaatac gaggtggttt tacaggtttc attgttaatt cctgattcag
     3121 gtgtgatttt ccctacgcca gatggataat tagcgcacag agactcacga aatattttta
     3181 ttaaatacgg ttgaatatgt ttttttagtt cggcggggga ggatattccc gccgaaatat
     3241 tattgcttat gcctgatgta aaatagttaa tactgtgcgc aaatcattta ccgagatttg
     3301 ccctggcgca gaggcttttt ttaccgcacc aaaagttgcc gccgagccaa atacttcacc
     3361 agccagacga gaaattacgc cagtttttgc cattgacatc gtgatgattg gacgatcggc
     3421 atactgctcc tgcatctcca gggtcgcggc aagcaacgtc agcacatcgc tggtactttg
     3481 cggcatcagc gcaatcttag gaatatcggc gtcgaaggac tgcattttgc gcagacgggc
     3541 aatgatttct tcggcttccg gcgttttatg gaagtcatgg ttggacatga caactttcac
     3601 atcatgcgcg tgggcgtagg cgacggtttc tttaacctga tcatcgccgg taaataactc
     3661 cagatcgatc atatcaacca ggccgctgtc gatggctgca cgattgagag caatataagc
     3721 ctcggtggaa atcgcctgct cgccgccttc tttggcactg cggaaggtaa acagcagcgg
     3781 tttttctggc atggtttcac ggagaatttt tgccgccgcc atgacagact ccacattgga
     3841 gaggtcggca aagtggtcca cacgccattc cagaatatca aagtccgctt cacgataggc
     3901 gagagcttcg gatttcacgc gggcgatatc tttcgccatc agcgagacga tgattttagg
     3961 tgcgcccgca ccaatgacga gatcttttac agttacggtt ttcatttttt accctttctg
     4021 cacgcggtca gcctgtcagg caccgaaccc catgacctgt ttaacatatt ccagagggaa
     4081 atctttgcca gtccataacg tgaactgttc agccccttgc cacaacaaca tgccgtatcc
     4141 atcaatcgtt ttgcaaccag cttgttgcgc ctgctgcaat aacttcgtca tatgcgggtt
     4201 atacacgcat tcagtgacca gcagtcccgg atgtaacaga ctgatatcat taaccaatga
     4261 ttcattctca aggggtttca tacccacttt tgtgccattg gttaaaatgt cggcggaagc
     4321 cagggcttca gcaaaggctt gctgatcggc gagatcggta accgtgacga cacaatcggt
     4381 gttttcgtta acccgctgcg cgaaggcgag ggctttatcg aagaactcat cccgacggtt
     4441 aaagagttta atttctttta aaccttcaat tgccccctgc gcgccaatcg ccgttgaggc
     4501 acctccggcc cctaacagca ccattgtttt gcctttgata tcaaaaccgc tctctttaat
     4561 tgcgcgaata tggcccgtgc cgtcggtgtt atagccgcgc aggtagccat catcattaac
     4621 gatggtgttg atggcaccca ccagtttggc tgctggcgtt aattcatcaa catattcaca
     4681 cgccagttgt ttgttcggca ttgatacacc agttccgcgc atttttagag cttttaatcc
     4741 ttcaattgct ccgggaaagc tatcgttatc cacttcgaag gccatatagg taaatggcaa
     4801 tcccgctttt tctaaggctt tattctgcat ttcaggcgat aaactgtggc ggataggata
     4861 agccatcaac ccaatcaatt cgtattttgc ggtaacatcc ataattaact cctttaacct
     4921 ctatgcttaa ttgtgtttaa ccgggtgcta aatttgcgct cgccaaaccg cacatcattc
     4981 tcaggaataa taaaaacgcg gtaatagcgg ataaaaacaa taattgcggt aatcagggcc
     5041 agcagcgcga aagtaaaatc gagaacaatg atatattgca ggccgatgtt cgacagataa
     5101 ccggtaatca gcggaataac aaagttagcc agtccaccca tcatcatata aatactggtg
     5161 actttggctt tgctttttgg gaaaaactct gacatgaccg aaacaccgag ctgtaaaatg
     5221 ccgccagctg ctgagaaacc gataacaaag gcgccggcat tacacaccag tggagaaggg
     5281 tacaggtaga taatggctgc tgttattgtt gccagtgcag agttaaatac attagcccag
     5341 atgggccgga ccattttttt cagtagtgcg gcaaaaataa agacacagac caacgagccc
     5401 atactgtaat aagagatggt ttttaatgcc tcagcttctg acataccagc aaaagccatc
     5461 gcatatttgg gcatccacac cacaatcaca taaaaggtcg agaatgcggc tacaccgaac
     5521 agtaccgatg aaacaccttc cagccagact aacggtttgc tgttcatttg cggtaattca
     5581 ttagctacgc tggcgtccac caactggctg gggaatttgc ttttcaacag catcagcgtg
     5641 atcagtacaa acagaatacc cggaataatc agcccatagc cgtaccagat attattgagc
     5701 aacatatagc tcaccagcat tgggtagaac atttgcccga atgacaccat cgctttaacc
     5761 agtatgaccg ccgaaccaga ggctttcgga aagcattcca tgagcgcggg gtagccaccc
     5821 gtatccagcg ctgagttagc gatacctacg cacactgcca gaccgtaggc gagagttaaa
     5881 ttcgggcaag cgggaatacc aaagaagaat agcagataca ttattactgc cattaatatc
     5941 accgcccgac gaccaaactt atcggagatc acaccgaaga ataaaatact gatcaatcgc
     6001 cccaaaccga taccggaaat taagtaggca atgcccgcgt tgtcagtgga aaacttttcc
     6061 gccagagatg acatattttg ggcaagcgta ataacactaa tgccgtgcag gaagtagctg
     6121 aagtaaatac aaagaacagc caggatgaat ggcgtgctga aagccttatt ttgagacatt
     6181 ttcacagctc cttgctgaaa gataatttcg ttaccgtacg tattgttctc cgacggttta
     6241 cataattact atactgaaga tgattttccc tgggtacatg gcataaagtt tatgtttcca
     6301 ggaatattgc atgtcatagt ttgtttttga tcgcgatttt ccttagttag cgcagaaaga
     6361 gttatttatc cgtaagatct tgcatgacct gaaaagaata ttgcattacc cacccggagc
     6421 gacattttcc tttagcgagt gatgccgcgt ttttttgcgg ctgcgtaagc cgataaacag
     6481 tgccagtaaa aagccgatgg cagcgatggc ggtatcgaac cacataatat cggcaatact
     6541 tctttgggac agatgagccg taatcaacgg aatagtaaag gtcgcaatac tgcccgcact
     6601 gtaatagatc cctgtagctt tacctttagc gtaggggaaa cgttcagcca ttaacgtcag
     6661 gccaatttgc acaacacctc cagcagaggt aaaaccaatg acaaaagcaa atattatcac
     6721 cacataaaat gtgggatgca ggcagacggt aaacagagca ataaatgaga taaaggtgta
     6781 cagcatcagt aatgttgttg ggcgaacggt attacgaatg agtggagcgg taataaatac
     6841 acaaagcagc gaacccacgg tgtagatact gagtagtttg atcgacatag tgtatgacat
     6901 gcctgcaaca aattgtccgt actgtgccag ccactggcta accagataaa acgttgccat
     6961 tgagatatag ccatataagg tataactggc taaatcgata attgaacagc gatgttccgt
     7021 agagctggtg gttttcttta tgacaggtaa gcgacgaccc ggatggggtg ggaacgtaca
     7081 acggtataaa aacagagcgt taataaacat aatgcctgca gcaatcataa aggaccaacc
     7141 gaaccacagt tcagcccaca ctaacaggct aatgattagc ggtaataaaa attgtccgct
     7201 ggaaacaaat gctttaatta aaatattggc tgtcccaggt gagcgtggaa aagcttccat
     7261 caaactggga taagtgcctg catcgagaaa actgtttgcc attcccgcca gaaagccaaa
     7321 aacataagcg ataatgatgt tattggtctg caggatgcca aaaaagaagg ccatatagca
     7381 gcacatcccg agcatgataa aagggcggcg accaaagcga tcggataata atcctgcaaa
     7441 aagcaagaca cttaatcgac caatgcccag cgatgagata actatcgaga cacccgcggc
     7501 attagtctgc caaagtgtct ccagcgaggc catattcagg ctcatcaaaa tgacgcccat
     7561 accatgcacc aggtaattaa aatacaaccc aagtgcggta gggtaatagg gatttttcat
     7621 aggttctact tagcaaaggt gctttaaaaa gtgtagcgat gttgtgtcag tatccattca
     7681 ttattgttgt aacaacgaca attcctttct gtggaaaaat taacaaagcc gctccagatc
     7741 gtgtgcatat aattcagcgg ctaccgattg atagatcttc cagtcaataa aattgccatc
     7801 agggtagact cgttgaaatg cggttaagtc ggggaaaaaa cgcatggtgt tgttgccgat
     7861 gcggttattt attttctcta tgatggcatg aagatgtttt tttctttgct gacgcatagc
     7921 caacagttgc tcgactacac aatcaggaat ggcacacttg ccattttccc attgacgcca
     7981 gctttcactg tcaccggtct gggcaatcca ggtcgcgcat tcatcaatag tcatggcaaa
     8041 aatatggcgg agtgcctgga gttcataagc gttcatcgtg taatggctgc atgaaataaa
     8101 caataaaata taaataacat tatcagcctg ctgacggctt gaagagagtc acagtatctt
     8161 gtgcaacatt attggaaaga gtgaggggcg aggtagcgaa gcggaaaaat gttaaaaaaa
     8221 agcccatcgt gggagatggg caaagactac acacagcaat tcgttgtttc actcagggga
     8281 tttccatgct tataaatcaa tatgttgatt tataaccgtg agctaatagt aggcatacgg
     8341 actttttgcg tcgatcagat tcgtctcatt agtttaaaaa ctgtaaagaa tttgcgacag
     8401 gtactaccag ctgaatggcg gtaagcagga aaatatgggg aagagcaagc aaatatcaat
     8461 tagcggtggt taacaatcag gcggcagagc cgcctgatga gaaattactt attcggtttc
     8521 tcgatttcgc caagttcttc gagaatttct tccggttggt ccgtcggcgg cgggacttct
     8581 tccacccacg cggcaaacag acgccaggaa acggctaaca gaaccggacc aataaacaga
     8641 ccgatcatcc cgaaagcaat caaaccacca ataacgccag agaggatcag gatcagcggt
     8701 aaatcggcac ccatgcgaat taacattggg cggatgacgt tatccagcgt gccaaccaca
     8761 ccgctccaca ctaacaatac cgttccccag gtggtatcgc cggtccagta gagccagata
     8821 atcgccggaa tcagtaccgg caacgggcca agctggacaa ggcaggagag gatcattaac
     8881 accgttagca acgttgcata aggtacgccg gatacggcga ggccgatacc gccaagcact
     8941 gcctgtacta acgccgtcac caccacaccc agcgccaccg cgcggatagc ctgtgccgcc
     9001 agcagcacgg cggcatctcc gcgaacgcct gccagacggg tcgcaaaatg gcgaatgcct
     9061 tgtgccacct gttcaccgcg ccagtacagc agggcactga agagaagcat cagcgcacaa
     9121 tgcaccataa agcgcccgat atgcgccgcc tgcccaacga accaggtggt ggtggtgcca
     9181 atataagggc ggaccttcgc catgatcgcc gtacccccca tatccagcaa gttgtgccag
     9241 cctgcataca gcttcgcgcc aatcaccgga atggtattaa gccacgccag atcgggtaac
     9301 gtcatgtcac cgctggaaat ggctttaatt agcgggccgc tgccgtcgac gatactgtta
     9361 accagcaaag caatagggat gataaacacc atcactaata acagcgtcat caccagaacg
     9421 gcgagagagc ggcggccaaa catgatcttt tgcaaacgta acaataccgg ccaggtggcg
     9481 ataaccaccg taccggccca tgcaaagccg agaataaagg gttgaacaat ccacagacat
     9541 gccacaatca tgatggctaa aaacagcacc gaaagcagaa tttgtgcgac atccctgggc
     9601 tgacgaacat ttaccataac tacttttcac cttttttgtg cgtcaaatcg tcgacgcgac
     9661 gtgaacacgc gaaactcaca tgcataatga tgagcaattt cagcggtttt taacaggccg
     9721 attctgccct taattctgat gggcgtgtta gaaaaaaatg tggtacaaca actcaggcaa
     9781 cacgcaaacg atttactcgt cgtatttcaa gttgcatatg ctgcggctac gttcgttcac
     9841 cccagttact tactgttgta agctcctgga gttcactcgc ttgccgcctt cctgcaactc
     9901 gaaatccata gagtaaataa acatcacctt tctgtgcgct attgcagccg cctgaaagaa
     9961 cagtgtatcg acctacatca cagaccgcag gaaagggtca atataatgat tccacagatt
    10021 tcccaggcac ccggcgtcgt tcaactggtg cttaattttt tgcaagagct ggagcaacaa
    10081 ggttttaccg gcgatacggc gacaagttat gccgatcgtc tgacaatgtc gaccgacaac
    10141 agtatttacc aacttctccc cgatgcggtg gtatttccgc gttcaaccgc agatgtggcg
    10201 ctgatcgccc gtcttgccgc gcaggaacgc tattcatcgc tgatctttac cccccgcggc
    10261 ggcggcaccg gcactaacgg tcaggcgctc aaccagggga ttattgttga tatgtcccgc
    10321 catatgaacc gcatcatcga aattaaccct gaagagggct gggtgcgcgt tgaggccggg
    10381 gtgataaaag accaactcaa tcagtacctg aaaccgttcg gctacttttt tgcaccagaa
    10441 ctttcgacca gcaaccgggc aacgctcggc gggatgatca ataccgatgc atccggtcag
    10501 ggatcgctgg tctatggcaa aacgtcagat cacgtacttg gcctacgtgc ggtattgttg
    10561 gggggcgata ttctcgatac gcaaccttta cccgtcgagc tggcggaaac gctgggtaaa
    10621 tccaatacca caatcgggcg aatttataac acggtttatc aacgttgccg tcagcaacgc
    10681 cagttaatta tcgacaactt ccccaaactt aaccgctttc ttaccggtta cgatctgcgt
    10741 catgtcttta acgatgagat gaccgagttc gacctgacgc gcattctgac gggttcagaa
    10801 gggacgctgg cctttattac cgaagcgcgg ctggatatta cgcgcttgcc taaagtgcgc
    10861 cgtctggtga atgtcaaata tgactctttt gactctgcgc tgcgtaacgc gccgtttatg
    10921 gttgaggcgc gggcactttc ggtagagacg gtggactcaa aagtgctgaa tctggcgcgg
    10981 gaagatattg tctggcattc cgtcagcgag ttgattaccg atgtgcctga ccaagagatg
    11041 ctcgggctga acattgtgga atttgctggt gatgatgagg cgctgattga cgagcgggta
    11101 aatgcactct gtgcgcggtt ggatgagctg atcgccagtc atcaagcagg tgtcatcggc
    11161 tggcaggtgt gccgtgagct ggcgggggtt gaacgtatct atgcaatgcg caaaaaagcc
    11221 gttggtctgc ttggcaatgc caaaggtgcc gctaagccaa ttccgtttgc tgaggatacc
    11281 tgcgtaccgc cggaacatct ggccgattat attgctgaat ttcgcgcgct gctcgacagc
    11341 cacggcttaa gttatggcat gtttggtcat gtcgatgctg gggtgttgca cgtccgtccg
    11401 gcgctggata tgtgcgatcc acaacaagag attttgatga agcaaatctc tgatgacgtg
    11461 gttgcgctga ctgcgaaata cggtggtttg ttgtggggcg aacacggaaa aggttttcgt
    11521 gccgaataca gcccggcgtt ttttggggag gaactttttg cagaactgcg caaagtgaaa
    11581 gcggcatttg acccgcataa ccgactcaac ccagggaaga tttgcccgcc agaaggtctc
    11641 gatgcgccga tgatgaaagt ggacgcggtg aagcgcggta cattcgatcg gcagatcccc
    11701 attgcggtac gccagcagtg gcgcggtgcg atggagtgta acggcaacgg tttatgcttc
    11761 aactttgatg cccgtagtcc gatgtgtccg tcgatgaaga tcacccagaa ccggattcat
    11821 tcaccgaaag ggcgcgcaac gctggtgcgt gaatggctgc gtttgttggc ggatcgcggc
    11881 gttgacccac tcaaactgga acaagaactg cctgaatccg gcgtcagttt gcggacgtta
    11941 attgcccgca cgcgcaatag ctggcatgcg aataaaggcg aatatgactt ctcacacgaa
    12001 gtcaaagagg cgatgtcggg ctgtctggcc tgtaaagctt gttcgaccca gtgccccatc
    12061 aaaattgatg tgccggagtt tcgctctcgt ttcctgcagc tctatcacac ccgttattta
    12121 cgcccgctgc gcgaccacct cgtcgctacg gtcgagagct acgcgccgct gatggcacgc
    12181 gcgccgaaaa cctttaactt cttcattaac cagcctctgg tgcgcaaact ctcggaaaaa
    12241 catatcggca tggttgattt gccgctgctg tcggtcccct cgctacaaca acaaatggtg
    12301 gggcatcgct cggcaaacat gacgctggaa cagcttgaat ctctcaatgc agagcagaaa
    12361 gcgcgcacgg tgttggtggt gcaggacccc tttaccagct attacgatgc gcaagtggtg
    12421 gcggattttg tccgtctggt cgaaaaatta ggtttccagc ctgtgttact gccattttcg
    12481 ccaaatggca aagcccagca tattaaaggt tttcttaatc gttttgcgaa gacggcgaaa
    12541 aagacggcgg atttcctcaa ccgtatggcg aagctgggta tgccaatggt gggcgtcgat
    12601 ccggcgctgg tactttgtta tcgcgatgaa tataaactgg cgctgggcga ggagcgtggc
    12661 gagtttaacg tcttactggc gaatgaatgg ctggcaagcg cacttgagtc acagccagtg
    12721 gctacagtca gcggtgaatc atggtatttc tttggtcact gtaccgaagt taccgccttg
    12781 ccgggtgcgc cagcacaatg ggccgcgata tttgcccgtt ttggcgcgaa actggaaaat
    12841 gtcagcgtgg gttgctgcgg catggcaggg acttacggac atgaagcgaa aaaccataaa
    12901 aattcgctcg ggatctatga gttatcctgg catcaggcta tgcagcgact gccgcgtaac
    12961 cgctgtctgg cgaccggata ttcctgccgt agtcaggtca aacgggttga aggcacgggg
    13021 gtacgccatc ctgtgcaggc tttactggag attattaaat gatatggaaa cgaaaaatca
    13081 ccctggaagc actgaatgcc atgggtgaag gaaacatggt ggggttactg gatattcgct
    13141 ttgaacatat tggtgatgac acccttgaag cgacaatgcc agtagactcg cggacaaagc
    13201 agcctttcgg gttgctacat ggaggtgcat ccgtggtact ggccgaaagt atcggttccg
    13261 ttgccggtta tttatgtacc gaaggtgagc aaaaagtggt tggtctggaa atcaatgcta
    13321 accacgtccg ctcggcacga gaagggcggg tgcgcggcgt atgcaaaccg ttgcatctcg
    13381 gttcgcgtca ccaggtctgg cagattgaaa tcttcgatga gaaagggcgt ttgtgctgtt
    13441 cgtcacgatt gacgaccgcc attttgtgac gttgtcacca gaaaagtgtg acgcatattg
    13501 cttttggtta ttatctgcac agtaaacggt ggtatactgt gcaggtcttg tctaaaagcg
    13561 aggatgctat gtctactcaa cttgatccca cccagctggc cattgaattt ttacgccgtg
    13621 accagagcaa tctttcacct gcccaatatc tcaaacgtct gaaacaacta gaacttgaat
    13681 ttgctgacct gcttactctc tcttctgctg aattaaaaga agaaatctac tttgcctggc
    13741 gtctgggcgt gcattgatcc tgacttaatg ccgcaagttc gccaaatcga cctggattaa
    13801 cctccgtcca ggttcgatga ccttcttaat actgcttatc tacccgcctg atattgttca
    13861 aaaataatac taatcgcagt ttgtgttaaa acgacggctt agctttatga gattattctt
    13921 atcgcccctt caagagctaa gccactgaga gtgccggaga taagcgccgg atggggtaga
    13981 aacccttaag cctgtgtcgc acagacttaa gggttttctt atttctgaat gctgcacttt
    14041 tttacctatc tcttaaatag tcattttcaa tacaactttt tctttttcat cacagcctta
    14101 atgctcctta gataataacc attatctaac aatgagatac ctaattctta gcgtgcctgt
    14161 taacccacac atcagggtct atgcttatta aaagcaggca aaaagcatgt tttacggtaa
    14221 agcccctgcg tttgctgggt tgaactgata atcattatca ctaacatgct gttatacgct
    14281 gaaagcgatg aagtgaggta aatcgatgga catgcattca ggaaccttta acccacagga
    14341 tttcgcctgg caaggcttaa cgctgacacc cgcagcggcg atacacatcc gcgagctggt
    14401 ggcaaagcag ccaggtatgg tcggcgtgcg cttaggtgtg aagcaaacgg gctgcgcggg
    14461 ctttggctat gtgctcgaca gtgttagcga gccggacaaa gacgatctgc tgtttgaaca
    14521 cgacggcgcg aagctgtttg tcccgctgca agcgatgccg tttattgatg gcacggaagt
    14581 cgatttcgtt cgtgaaggac ttaatcagat attcaaattt cacaacccta aagcccagaa
    14641 tgaatgtggc tgtggcgaaa gctttggggt ataggcggta ctatgtctcg taatactgaa
    14701 gcaactgacg atgtcaaaac ctggaccggc ggcccgctga attataaaga aggattcttc
    14761 acccagttag ccaccgatga gctggcaaag gggataaacg aagaggtggt gcgcgcaatt
    14821 tcggcgaagc gtaatgagcc ggagtggatg ctggagtttc gtctaaacgc ctatcgcgca
    14881 tggctggaga tggaagaacc gcactggttg aaagcgcact acgacaagct gaattatcag
    14941 gattacagct actactcagc accatcgtgc ggtaattgtg acgacacttg cgcgtctgaa
    15001 cctggcgcgg tgcagcaaac tggcgcgaac gcctttttaa gtaaagaggt ggaggcggcg
    15061 tttgagcagt tgggcgttcc cgtgcgggaa ggcaaagagg tggcggtgga tgccattttc
    15121 gactcagttt cggttgccac tacttatcgc gaaaaactgg cggagcaggg aattattttc
    15181 tgttcctttg gtgaggcgat ccacgatcac ccggaactgg tgcgtaaata tctcggcacc
    15241 gtggtgccgg ggaatgacaa cttctttgcc gcgcttaatg cggcggtagc ctctgacggt
    15301 acgtttattt atgtgcctaa aggcgtgcgc tgcccgatgg aactttccac ctattttcgc
    15361 attaacgcgg aaaaaaccgg gcagtttgag cgcaccattc tggtggccga cgaagacagc
    15421 gacgtcagct acattgaagg ctgttccgct ccggtgcgtg acagctatca gttgcacgcg
    15481 gcagtggtgg aagtcatcat ccataaaaac gccgaggtga aatattccac ggtacaaaac
    15541 tggttccctg gcgataacaa caccggcggt attctcaact tcgtcaccaa gcgtgctttg
    15601 tgcgaaggcg aaaacagcaa aatgtcatgg acgcaatcag aaaccgggtc agcgattacg
    15661 tggaaatatc ccagctgcat tttgcgcggc gataactcca ttggtgagtt ttactcagtg
    15721 gcgctgacca gcggtcatca gcaagcggat accggcacca agatgatcca catcggtaaa
    15781 aacaccaaat cgaccattat ctcgaaaggg atctctgccg gacatagtca gaacagttat
    15841 cgcggcttag tgaaaatcat gccgacggca accaatgcgc gcaatttcac tcagtgcgac
    15901 tcaatgctga ttggcgctaa ttgtggggcg cataccttcc cgtatgtcga atgtcgcaat
    15961 aacagcgcac aactggagca cgaggcaacg acatcacgta ttggtgaaga tcaactgttt
    16021 tactgcctgc aacgcgggat cagcgaagaa gacgccatct cgatgattgt taacggtttc
    16081 tgcaaagacg tgttctcaga actgccgctg gaatttgccg ttgaagcaca aaaactcctc
    16141 gccatcagtc ttgaacacag cgtcggataa ggaataaaca tgttaagtat taaagattta
    16201 cacgtcagcg tggaagataa agctatcctg cgtggattaa gcctcgacgt tcgtcccggc
    16261 gaagttcacg ccattatggg gccaaacggt tcgggcaaaa gtaccttatc ggcaacgctt
    16321 gccgggcgag aagattatga agtgacgggc ggcacggttg agttcaaagg caaagatttg
    16381 catgcactgt cgccggaaga tcgtgcaggc gaaggcatct ttatggcctt ccagtatccg
    16441 gtggagattc caggcgtcag taaccagttt ttcctgcaaa cggcacttaa tgcggtgcgc
    16501 agctatcgcg gccaggaaac gctcgaccgc tttgattttc aggatttgat ggaagagaaa
    16561 atcgctctcc tgaagatgcc ggaagattta ttaacccgtt cggtaaacgt tggcttttcc
    16621 ggcggcgaga aaaagcgcaa cgatattctg caaatggcgg tgctggaacc ggggttatgc
    16681 attcttgatg aatcggactc cgggctggat attgacgcat taaaagtggt tgccgatggc
    16741 gtgaactcgc tgcgtgacgg caagcgctca ttcatcattg ttacgcacta ccaacgcatt
    16801 ctcgactaca tcaagcctga ttacgttcat gtgctatatc agggacgaat tgtgaaatcc
    16861 ggcgatttca cgttggtcaa acaactggag gagcagggtt atggctggct taccgaacag
    16921 cagtaacgcg ctgcaacagt ggcatcactt gtttgaagct gaagggacaa aacgctcccc
    16981 gcaagcacag cagcatttac aacaattgct gcgtaccgga ctgccgacac gtaaacatga
    17041 aaactggaaa tatacgccgc tggaagggct gaccaatatc cagtttgtca gcattgtggg
    17101 ggag
//
LOCUS       DAZUCE010000103        17012 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZUCE010000103 DAZUCE010000000
VERSION     DAZUCE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.103"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            52..321
                     /locus_tag="ACHY03_002820"
     CDS             52..321
                     /locus_tag="ACHY03_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFS3906028.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGEAGIAPEIHTGDDDALKEILTHVTEATKKQY"
     gene            290..1408
                     /locus_tag="ACHY03_002821"
     CDS             290..1408
                     /locus_tag="ACHY03_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFS3906029.1"
                     /translation="MSPRPRKNSTDVAALYEKFDRRTGKVYYQYKNPVTGKFHGLGTD
                     KVKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDSRLKN
                     GDIAATTHKEKVRMAAYLVSRLGNHPLKELEVRDFALILDEWMDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRSRLTLEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNGHLHITQGKTGMRLALPLTLRCDEIGVSLKEVIDGC
                     RDRMLSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1685..2242
                     /locus_tag="ACHY03_002822"
     CDS             1685..2242
                     /locus_tag="ACHY03_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFS3906030.1"
                     /translation="MTTQIPVETLSPIAHNQIPVITTELLAHLYGADVNSIQQNHKRN
                     SEHFIEGKHFFKVVGEELKNLRLTISQLQISPKTRSLILWTERGAARHAKMLETDQAW
                     EVFEKLEDSYFSQKQPPATQKSPIENDGCALLIHFNNYGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVTRKEQLVERLMRL"
     gene            2395..3189
                     /gene="potC"
                     /locus_tag="ACHY03_002823"
     CDS             2395..3189
                     /gene="potC"
                     /locus_tag="ACHY03_002823"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFS3906031.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            3186..4232
                     /gene="potD"
                     /locus_tag="ACHY03_002824"
     CDS             3186..4232
                     /gene="potD"
                     /locus_tag="ACHY03_002824"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFS3906032.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            4290..4751
                     /gene="ymfA"
                     /locus_tag="ACHY03_002825"
     CDS             4290..4751
                     /gene="ymfA"
                     /locus_tag="ACHY03_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFS3906033.1"
                     /translation="MSQDSKVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVLHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPTSKKSRRKRKSRP"
     gene            4748..5533
                     /locus_tag="ACHY03_002826"
     CDS             4748..5533
                     /locus_tag="ACHY03_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFS3906034.1"
                     /translation="MKKIIILLSLLILLPLTVTSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQIGIAPFIVVVPGVNEASFRQNGKAMLAHASSSLSDVKGSVLLLFTTRE
                     PRLIMITNGHVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPTP
                     PLTYYPHPGQQQENDPPGSTTTLGLFAWAVAFIVFTAFFNYTTRLNYALKFAVAMTIA
                     NMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(5670..6491)
                     /gene="cobB"
                     /locus_tag="ACHY03_002827"
     CDS             complement(5670..6491)
                     /gene="cobB"
                     /locus_tag="ACHY03_002827"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFS3906035.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(6507..7418)
                     /gene="nagK"
                     /locus_tag="ACHY03_002828"
     CDS             complement(6507..7418)
                     /gene="nagK"
                     /locus_tag="ACHY03_002828"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFS3906036.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(7447..8691)
                     /gene="lolE"
                     /locus_tag="ACHY03_002829"
     CDS             complement(7447..8691)
                     /gene="lolE"
                     /locus_tag="ACHY03_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFS3906037.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(8691..9392)
                     /gene="lolD"
                     /locus_tag="ACHY03_002830"
     CDS             complement(8691..9392)
                     /gene="lolD"
                     /locus_tag="ACHY03_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFS3906038.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(9385..10584)
                     /gene="lolC"
                     /locus_tag="ACHY03_002831"
     CDS             complement(9385..10584)
                     /gene="lolC"
                     /locus_tag="ACHY03_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFS3906039.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10846..11919
                     /gene="ycfT"
                     /locus_tag="ACHY03_002832"
     CDS             10846..11919
                     /gene="ycfT"
                     /locus_tag="ACHY03_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFS3906040.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMSCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            12047..15493
                     /gene="mfd"
                     /locus_tag="ACHY03_002833"
     CDS             12047..15493
                     /gene="mfd"
                     /locus_tag="ACHY03_002833"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFS3906041.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKHW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            15637..16599
                     /gene="ldtC"
                     /locus_tag="ACHY03_002834"
     CDS             15637..16599
                     /gene="ldtC"
                     /locus_tag="ACHY03_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFS3906042.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(16682..16939)
                     /gene="bhsA"
                     /locus_tag="ACHY03_002835"
                     /gene_synonym="ycfR"
     CDS             complement(16682..16939)
                     /gene="bhsA"
                     /locus_tag="ACHY03_002835"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFS3906043.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4317 a         4354 c         4282 g         4059 t
ORIGIN      
        1 tcgccggttg tagttggtat tctgcaactg gcgcgttccg gagtgatggt catgagcgaa
       61 cagtacctga ttacgctcga cgagtggaaa cctaagcggt tcagtctccc aataacaaac
      121 actaccctgg tgaaatacgg aaaacttgga tacatcgtac caagaccaca aaaaattcgt
      181 ggtcgctggc taatagatcg ccgcgcagtc tttgttggac cgggtgaagc tggaattgcg
      241 ccggaaattc atactggcga tgatgacgca ctgaaggaga ttttaaccca tgtcaccgag
      301 gccacgaaaa aacagtactg acgtagcagc tctttacgaa aagtttgatc gcagaactgg
      361 caaggtttac taccagtata aaaatcctgt gactggaaaa tttcacggac tcgggacaga
      421 caaagtcaag gcagaaaaaa tcgcttcaac agccaatcag cgaatagctg cagcagaagc
      481 agaatatttc atgcgcaaaa ttgatgaaag tccatcagca acaaaacgtc ggggtatcag
      541 attaaaggca tgggttgatc gatatctgaa aatacaggat tcgcgactga aaaatggaga
      601 cattgcagct acaacccaca aagaaaaagt gcgaatggct gcatacctgg tttcccgcct
      661 gggaaaccac ccattgaaag aactggaggt aagagacttt gcattaatac tggacgagtg
      721 gatggataaa gacatggtca gcacagcaag agtaaatcgc ggattatggg ttgatattta
      781 taaagaagca cagcatgcgg gagaagttcc tcctggatgg aatcctccgg aggctacccg
      841 taaaccgatc cctaaagtga ccagatccag actcaccctg gaagactggc aaaaaattta
      901 taatgcaacg ccagaaaaac actttatccg taacgcaatg cttcttgcga ttgttactgg
      961 tcagcgccgt gatgacattt gccacatgcg tttttcagat gtatggaatg gacatctaca
     1021 tataacccaa ggaaaaaccg gaatgcgtct ggccttacct cttacgctac gatgcgatga
     1081 aattggggta tcgttaaaag aagttattga tggatgcagg gacagaatgt tgagtccata
     1141 cctaatccat agtagacacc agaaacaacc gaagccgatg agtaaagaca acctgagcga
     1201 ctactttgcc aaagcacggg atctggctgg gataattcca ccagcaggaa aaactccgcc
     1261 aacatttcat gaacaacgct ctctatcaga acggctgtac cgtgcacagg gtatcgatac
     1321 aaaaacatta ctaggacata aagtccaggc aaccaccgat cgctataacg atactcgagg
     1381 tcaggaatgg gttaagttgg ttatttgaca aaaataaccg tccgggatta tagtctgcgc
     1441 acgccagcaa aatctggcgt cgggattagg aaccccggat attcaaccgc gacagaacac
     1501 gccgcgagcg tgttttttat tgtcgtaagc gcacgcacat ctgaataatg gtggggcgta
     1561 tgggggagcc gaaaggctcg ccggttggtt gatccggtag ttcctaaccc tgtacgtctc
     1621 gccacccgat gattaggaac ctgaaggtgg tgatgttttg aaacatcaac cggagggcgt
     1681 caatatgacc actcaaattc ctgttgaaac tctttccccg attgcccaca accagattcc
     1741 tgtcataacc accgagttat tggcgcatct ttatggtgcg gatgtgaata gtattcagca
     1801 aaaccataag cgcaattcag agcattttat tgaaggtaaa catttcttca aggttgtggg
     1861 cgaagagctt aaaaatttgc gactgacaat aagtcagtta caaatctcgc ccaaaacccg
     1921 ctccctcatt ctctggacag aacgcggggc tgcccgtcat gcaaaaatgc tcgaaacaga
     1981 tcaggcatgg gaagttttcg aaaaactgga agacagttat ttcagccaga aacaaccacc
     2041 agccacacaa aaatcaccca tcgaaaacga cggatgcgca ttactgatcc atttcaataa
     2101 ctacggacag gtcgagttca cggaaaaagt gcctgccgat gcgatggtgt gtactctgga
     2161 gcgttttaag ttttatctgg agcagcacgg ctggatcgtt acccgtaagg agcaactggt
     2221 agaaaggctg atgcgacttt aaaaatgagg ggctcgcatc aggaaaaatg ttgtagaatg
     2281 cgagcccctc cgttttgggg aaaagttttg gagagatttt ggagaaagga aaaaattgaa
     2341 taaattcaaa atcctgaaag tgccccgcct tatgaataaa aaggtggaac tcgaatgatc
     2401 ggtcgactgc ttcgcggcgg ttttatgacc gctatctacg cgtacctgta tatcccaatc
     2461 attattttga ttgtgaactc ctttaacagc tcgcgctttg gcatcaactg gcagggtttt
     2521 accactaaat ggtatagctt gctgatgaac aacgacagcc tgttacaggc agcgcagcat
     2581 tcactaacaa tggcggtgtt ttcggcgacg tttgctacgc ttatcggttc actgacggca
     2641 gttgcactgt accgttatcg ctttcgtggt aagccgttcg ttagcggaat gctgtttgtg
     2701 gtaatgatgt caccagatat cgtgatggca atttcgctgc tggtgctgtt tatgctgctg
     2761 ggtattcagc ttggcttctg gtcgctgctg ttctcgcata tcaccttctg cctgccattt
     2821 gtggtggtga cggtgtattc gcgcctgaaa ggttttgacg tgcgaatgct ggaagcggcg
     2881 aaagatctcg gtgccagcga atttaccatt ctgcgaaaaa tcattctgcc actggcaatg
     2941 ccagcggtgg cggcgggctg ggtgttaagc tttaccctgt cgatggacga tgtggtggtt
     3001 tcttcgttcg tcaccgggcc gagttatgaa attctgccgt taaaaattta ttcgatggtc
     3061 aaagtcggcg tatcgccgga agttaacgcg ctggcaacca tattactggt gctgtcgctg
     3121 gtgatggtta ttgccagcca gcttattgct cgtgataaaa cgaaaggtaa cacaggggac
     3181 gttaaatgaa aaaatggtca cgccacctgc tcgcggcggg tgctctggca ctgggcatga
     3241 gcgccgctca cgccgatgac aacaacacgc tgtatttcta caactggacc gagtacgtgc
     3301 cgccaggact gcttgaacag ttcaccaaag aaaccggtat taaggttatc tattcgactt
     3361 acgagtcgaa cgaaaccatg tatgccaagc tgaaaacgta caaagacggt gcctatgacc
     3421 tggtggttcc ttcaacctat tacgtcgata aaatgcgtaa agaagggatg atccagaaga
     3481 tcgacaagtc gaagttaacg aacttcagca atctcgatcc agacatgctc aataagccgt
     3541 ttgacccgaa taacgattac tccattccgt atatctgggg tgcgacggcg attggcgtta
     3601 acggtgatgc ggtggatccg aaatctgtca ccagctgggc cgatttgtgg aagcctgagt
     3661 acaaaggcag cctgctgctg accgacgatg cccgtgaagt gttccagatg gcgctacgta
     3721 agctgggcta ctccggtaac accaccgatc cgaaagagat tgaagctgca tataacgagc
     3781 tgaaaaaact gatgccaaac gtcgcagcgt ttaactccga taacccggct aacccgtaca
     3841 tggaaggcga agttaacctc ggcatgatct ggaacggttc tgctttcgtt gcacgccagg
     3901 cgggtacgcc tattgacgtg gtgtggccga aagaaggcgg catattctgg atggacagcc
     3961 tggcgatccc ggcaaatgcc aaaaacaaag aaggcgcgct gaaattgatc aacttcctgc
     4021 tgcgcccgga tgtggcaaaa caggttgctg aaactatcgg ttatccaacg ccaaaccttg
     4081 cggcgcgtaa gctgttaagt ccagaagtgg cgaacgataa gacgctctac ccggatgctg
     4141 aaaccattaa aaatggcgaa tggcagaatg acgttggcgc agccagcagc atttatgaag
     4201 agtattatca gaagctgaaa gcaggacgtt aattcagcaa gcgggcggtg gtattaccgc
     4261 ccgtttttta gttagcaagg atgtggctaa tgagtcagga cagtaaagtt ttctttcgta
     4321 tattcttcgg tatcggtctg gtactgatcc tcatttctgt tgttatcttt tataaccagt
     4381 tcacctatag caaagacgcg attcataccg aaggcgtcat tgttgatacc gtcttgcact
     4441 ccagtcattc tcatcgcacg ggtaaaaatg gttcgtggta tcccgtcgtt gcctttcgac
     4501 cgacgccaga ttacacgcta attttcaatt cgagtattgg cagcgatttt tatgaagaca
     4561 gcgaaggcga taaagttaat gtttattatt ctcccgggca tcctgaaaag gctgagataa
     4621 ataacccgtg ggttaatttt tttaaatggg ggtttatcgg cattatgggc gttattttta
     4681 tcgccgtcgg tttattaatc agtatgccca cctctaaaaa atcacgcagg aaacggaagt
     4741 cccgtccatg aaaaaaatca taatactgct cagtttactg atacttctgc ctctgacggt
     4801 aaccagtaag cccttaatcc caataatgaa gacacttttt acggatgtta caggtactgt
     4861 accggatgct gaggagattg cgcggaaagc ggagcttttt cgtcaacaaa tagggattgc
     4921 gccgtttatc gtggttgtac ctggcgttaa tgaagcaagc ttcaggcaaa atggcaaagc
     4981 gatgctggcg catgcgtcat cttcattgag tgatgtaaaa ggaagtgttc tgttactttt
     5041 tactacacgc gagccacggt taattatgat aaccaacggc catgttgaaa gcagcatgga
     5101 cgataagcat ctcggccttc tggtagaaaa tcacacgctg gcttatttac atgcggatct
     5161 ctggtatcag ggaatcaata atgcattggc tgttttacaa gcacagatat taaaacaacc
     5221 gacgccgcca ctgacatatt atccgcatcc cggacaacag caggagaatg atcctcccgg
     5281 cagcaccacg acattaggcc tttttgcctg ggcggttgca tttatcgtct ttactgcatt
     5341 ttttaactac acgacgcgcc ttaattatgc attaaaattt gcagtggcga tgactattgc
     5401 caatatgggc tatcaggctt tatgcctgta tatcgataat agctttgcga ttaccagaat
     5461 atcgccactc tgggcagggc tgattggtgt ctgtacgttt attgccgcgt tgttattgac
     5521 gagtaaacgg taaaagagat agtaaaatgc gtgtgtatta tgccggatgc gacgcgaacg
     5581 ccttatccgg cccacaaccc cgcaaattca attaattgcg tccccttgta ggcctgataa
     5641 gcgtagcgca tcaggcaatg cttcctgctt tataatccct tcagcaactt ttcaacaaat
     5701 tctggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt accaacctga
     5761 ctcggttcaa gattcagttc cacggtgtgc gcaccatgca gtttcgcttc gtgaacaaac
     5821 ccagccgccg gataaacatg ccccgaagta ccaatggcaa tgaaaatatc ggccatcgac
     5881 aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccatac tacgtgtggg
     5941 cgcaaggggg ccgggaactg gcagcaatgg catttatctt ctggggtaac gtctccggtc
     6001 cagtcgagaa cctgaccact ttgagaacaa cgaactttaa gcagttcccc atgcatatga
     6061 atcacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt caccagcaaa
     6121 aagcgatcgc cgagagcatc ttgcagttta gccagcgcaa gatgcgcggc gttaggctga
     6181 atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac cagttcagga
     6241 tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc ccacaggcca
     6301 tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc tgtcagtacg
     6361 agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa aatacgctga
     6421 cgcaaacgct cgcgcaggcg gcgtttattt ttacgaaaac gacttaaccg atgaccccga
     6481 cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc
     6541 attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc tacaggtaag
     6601 agatgacgag gcagcctgtc cgccagttgc gttgtgattg ccgggaaatt cgataagcca
     6661 ccaccaatga cgaccaggtc agggtcaaca atggtcagga tatttcccag acaaaccgct
     6721 aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg atcataaagc
     6781 gcaataattt cgggagcctg caacggttga tgataatagt gttgatacag ccatgcaaaa
     6841 ccgcgaccag acagataatt ttcaatgcag ccatgctgac cacagccgca gcggcgtaac
     6901 gggaaatcca accccatcat ggttaacgca tcaaccggca gacgcatatg gccaaactcg
     6961 ccggtaatgt agcttttccc ggtaatcggt ttgccgttga aaatcagccc gccgccaacg
     7021 ccagtgccga gaatcaaccc catcaccagt ggatattgcg taaattcatc atcccaggct
     7081 tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc aagacgtgcg
     7141 ctcaggtcgg cacgcagcgg tttaccgctg gcagcgggga cattggcggc atacagcgta
     7201 ccatcttctg tttccggcat tcccggaata ccgatgccga cagaacctct acagccaaaa
     7261 cgttgatcag cttcggctac cagctcgcac actgcatcta aaaatgcgtc atagctatca
     7321 cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa cacgccaagc
     7381 gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg ttttaatgta
     7441 ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc gccgcgccgg
     7501 ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga agacgtccag
     7561 ccagtgcaat tccgatggca ggaaatcaat aaaatagata tcgctggaga ggaactgatg
     7621 accgatcagc ttttcaatcc gctcaataat cggggtaagt tgcagtgaaa caactacgcc
     7681 gataatcacc ccacacaggc tgccgaacag ccctgccagc aatccatacc agacaaagat
     7741 ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat cgccactctt
     7801 gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc caatcaccag
     7861 taccatcgcc agatacataa tggcgcggat catctgaata tcgcgataca tatagccgta
     7921 agtaccaatc cagcttttaa tataaacata gctgttggtc acttcacccg catcgcgtac
     7981 cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca cgctggaacc
     8041 catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt gatcgagttg
     8101 accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct gcatcagttt
     8161 atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct tcactttcag
     8221 cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa aattgcgcca
     8281 ggcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt gcgggttaac
     8341 gcccttcacc tggattgcgc gcagattggc accactttct accagtccgg tgaaattgat
     8401 atacggcgca gcggcggcaa tacccggcac tttttgcacg ttatccagtg cttcctgcca
     8461 gttagtccac ggctgatcta ccgcctcaat ttcaccgtgc ggtaccacag caagaatgcg
     8521 gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca ataccgccac
     8581 gccgagggca atgccaatgg tagaaatgac ggagatcagc gacaccatgc cgccgcgccg
     8641 ccgtccgcga ctaaaacgca ggccaattaa taacgataaa ggcatcgcca ttactccgcc
     8701 cccatcaggc tcagttccgc cgtcagacga ccatcacgca tttctaactg gcggctcata
     8761 cgtttcgcca gttgcaggtc gtgagtaacc accaggaagg cggtgccctg caagcgattc
     8821 aattctccaa gcaactggaa gatgctgtcg gcgttacgtg cgtcaaggtt accggttggt
     8881 tcatccgcca gtaccagacg cgggttattg accagcgcac gggcaatcgc cacacgctgg
     8941 cgttcgccgc cagaaagctc tgacgggcgg tgattcgcac gatgatccag ccccaccgct
     9001 tttaacatct caagtgcacg gctgttgatt tcagcgggct ttttcttgcc aatcagcagc
     9061 ggcatagcca cgttttccag ggcggtaaaa tccggcagca ggtggtgaaa ctgataaata
     9121 aagcccagct tctggttgcg cagttctgct ttcgcagccg acgacagttt gctcattggc
     9181 tgaccattaa agatcacatc gccggaggtt ggcgtatcca gcccgcccag caggtgcagc
     9241 aaggtacttt taccggaacc agagctaccg acgatcgcca tcatttcacc ttcgccgacg
     9301 ctgaaactga cattgtgcag cacatcggtt tgcacactgc cttcctgata gcgtttgcac
     9361 aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg gttgagtggc
     9421 ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg ccaccagcgc
     9481 aataacaatg acctgtaaag gttcgatagc gaccggcagc gccgcgccat caagcaggac
     9541 gccgattatc ggcatcagat tatttaactg gctggcaagc agggcgccaa gcgccgctcc
     9601 gaggatcgca ccgataatcc cggcgctggc cccttgcacc ataaagacca tcatgatttg
     9661 tcgcggagtt aagccttgcg tttgcaggat cgctacttcg ccctgcttct ccattaccat
     9721 cagccctagt gaggtaataa tgttaaacgc cgcaacggcg acaatcaggc tcagcagtaa
     9781 acccatcata tttttttcca tgcgtacggc ctggaacaac tcgcctttac ggtcacgcca
     9841 gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa ctttcagcgg
     9901 ctcatccagc cacaaacgcc agccggtaat attgcctgcc ggataacgca ttagacgcga
     9961 ggcatcctca atattcacca gcatttcata gccatcgact tcactgttag cggcaaaagt
    10021 accaatcaca ttgaacaggc gctgacttgg aatacgcccc atcggcgtga actggctggc
    10081 agatggcacc atcacgcgga tttgatcacc gcgattgacg cccaactgtg aggcaagctg
    10141 ttcaccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca cattgaccag
    10201 atacggcgtt agtggatctt tttgcgccgg atcgataccc agcatcaccc cgaccgccac
    10261 gctgcgcgcg ctttgcagta ccacatcccc ggtagtaata ggtgcgacgc gattaacgcc
    10321 gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat gctcagaaga
    10381 gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct caaagccgtt
    10441 catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga tggtagaaag
    10501 ccaggagacg aaacgaccga agcgatccgc tgcacgccca cgcatgtaac gcaggccaat
    10561 aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa gacacgagta
    10621 tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag ggctaatctg
    10681 aaaagccccc gtaaatcaat cggctaaatt gccatgtttt atcatcaatc ttgccgcctg
    10741 atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg ggtcataacc
    10801 cgcaggatca accgcatgta aattaaaaaa gagcgtgttg tcctgatgaa acaaaaagag
    10861 ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta tcactcggtc
    10921 attacctttt atccgcatat gaccactttt cagcatccgt tatcggaagt cctgagcaaa
    10981 tgctggatct atttcaatct ttaccttgca ccctttcgta tgccggtttt tttctttata
    11041 tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg tctcgataaa
    11101 cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg gctggcgtta
    11161 agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc caatgccgct
    11221 tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac cagcttgtgg
    11281 tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg tctggcgctg
    11341 ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc cacgccgtgg
    11401 tggggaatga acagtgtgat ccgcaatttg ccttattaca gccttggcgc atggtttggc
    11461 gcaacaataa tgagctgtgt taaagaggtg ccgttgcgcc gccatctgct gatggcttct
    11521 ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct gttgtcgctg
    11581 gtatcgattg tggtgatcat gaagctgttt tatcaatacg agcaacgatt cggtatgcgc
    11641 tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac cacccatcgc
    11701 attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc acgctggtcg
    11761 ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt catctgtact
    11821 gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct gttgttctcc
    11881 ccgccttcac tgcccgctgc cgtcagttac tcccgctaag ccatcgcccc agccggggcg
    11941 attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta tcagagatat
    12001 gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc ctgaacaata
    12061 tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt taaccggcgc
    12121 tgcctgcgcg acgctggtgg cggaaattgc cgaacgtcac gccggtccgg tggtactcat
    12181 tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt tcaccgatca
    12241 gatggtgatg aatctggcgg actgggaaac cctcccttac gacagttttt cgcctcatca
    12301 ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc agcgtggcgt
    12361 actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt ttctccacgg
    12421 tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac gaacccaact
    12481 ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat acgccacgcg
    12541 cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc ttgatttctt
    12601 tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca cgctggagga
    12661 agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag cggcaattga
    12721 actgttccgc agccagtggc gtgatacctt cgaagtgaag cgcgatccgg aacatattta
    12781 ccagcaagtg agtaaaggca cattacctgc cgggatcgag tactggcagc cgctattctt
    12841 cagcgaaccg ctgccgccac tgttcagtta tttccctgcc aataccttgc tggtgaatac
    12901 tggcgatctg gaaaacagtg ccgaacgttt ccaggctgat acactggcgc gttttgagaa
    12961 tcgcggcgtc gatccgatgc gcccactgtt gccaccacaa tcgctctggc tgcgggtgga
    13021 cgagctcttc tcagagctga aacactggcc ccgagtgcag ctaaaaactg aacatttacc
    13081 gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg ccgttcaggc
    13141 acaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg atggtccggt
    13201 ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc tcgcacgaat
    13261 taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg ggcgttatct
    13321 gatgattggc gctgccgaac atggttttgt cgatacggtg cgtaacctgg cgctgatttg
    13381 cgaaagcgat ctgctcggtg aacgcgttgc ccgccgtcgt caggactctc gccgcaccat
    13441 caaccccgat acactgatcc gcaacctcgc ggagctgcat attggtcagc cggtggtcca
    13501 tctggagcac ggcgttggtc gttatgccgg aatgaccacg ctcgaagctg gaggcattac
    13561 tggcgagtat ttgatgctca cctatgccaa cgacgccaaa ctgtatgttc cggtgtcgtc
    13621 actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc tgcataaact
    13681 tggcggtgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc gtgatgtggc
    13741 ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg cgtttaaaca
    13801 cgatcgtgag cagtatcagt tgttctgcga cagcttcccg tttgaaacca cgccggatca
    13861 ggcgcaggcc attaatgcgg tgcttagcga catgtgtcag ccactggcaa tggatcgtct
    13921 ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg ctttcctggc
    13981 agtagataac cacaagcagg tggcggtgct ggtgcctacc acccttctcg cgcagcagca
    14041 ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa tgatctcccg
    14101 tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag ggaaaatcga
    14161 tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag atttaggcct
    14221 gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca ttaaagcgat
    14281 gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgta cgctgaatat
    14341 ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc gtcgtctggc
    14401 agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga tcctgcgtga
    14461 aattttgcgc ggaggacagg tttattatct ctacaatgat gtggaaaaca ttcagaaagc
    14521 cgccgaacgg ctggcagaac tggtgccaga agcgcggatc gccatcggtc acgggcagat
    14581 gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt tcaacgtgct
    14641 ggtttgtaca accattatcg aaaccgggat cgacatcccg acagccaaca ccattatcat
    14701 tgaacgcgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc gcgtcggacg
    14761 ttcgcatcat caggcatatg catggttgct gacaccgcat ccaaaagcga tgactaccga
    14821 tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggtgcag gttttgcgct
    14881 ggcaacgcac gatctggaga tccgcggcgc gggtgaactg cttggcgaag aacaaagtgg
    14941 ctcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg ccgtcgatgc
    15001 actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa cagaagtcga
    15061 gctgcggatg ccgtcgctat tgccagatga tttcatccct gacgtgaaca cgcgtctgtc
    15121 gttctacaaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga tcaaagtcga
    15181 gcttatcgat cgcttcggcc tgctgccgga tccggcgcgt accctgctgg atattgcccg
    15241 tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg agaaaggcgg
    15301 cgtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg gtttgctgca
    15361 aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaagttta ttcaggattt
    15421 gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac tggaagagaa
    15481 cgcgatcgct taatggtaaa tcgggggcgt ttctgcgccc ccatctgcaa catttacaaa
    15541 ttctttgcac ttccctgcac tatccgacac tgtcaccatc cataattcag gcttatctgt
    15601 ttattacaat aaccttatat ttattatgga tttttggtga tgatcaaaac gcgtttttct
    15661 cgctggctaa cgttttttac gttcgccgct gccgtggcgc tggcgctacc ggcaaaagcc
    15721 aacacctggc cgctgccgcc agcgggcagt cgtctggttg gcgaaaacaa atttcatgtg
    15781 gtggaaaatg acggtggttc tctggaagcc atcgccaaaa aatataacgt cggctttctc
    15841 gctctgttac aggctaaccc cggcgttgat ccttacgtac cgcgcgcggg cagcgtgtta
    15901 acgatcccgt tgcaaaccct acttccagac gcgccgcgcg aaggcattgt gatcaacatt
    15961 gcggagctgc gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt gtatccaata
    16021 ggtattggtc agttaggtgg tgacacgctg acaccgacaa tggtgaccac cgtttcagac
    16081 aaacgtgcaa acccaacctg gacgccaacg gcaaacatcc gcgcccgtta taaagcacag
    16141 ggaattgagt tgcctgcggt agtgccggct ggaccggata acccaatggg ccatcatgcg
    16201 attcgtctgg cggcctatgg cggcgtttat ttgcttcatg gtacgaacgc cgatttcggc
    16261 attggcatgc gggtaagttc tggctgtatt cgtctgcggg atgacgatat caaaacactc
    16321 tttagccagg tcaccccagg caccaaagtg aatatcatca acactccgat aaaagtctct
    16381 gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga gaagattgat
    16441 gacgatccgc agctgctgcc aattacgctg aatagcgcaa tgcaatcatt taaagatgca
    16501 gcacaaactg acgctgaagt gatgcaacat gtgatggatg tccgttccgg gatgccggtg
    16561 gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa tcctggataa aaaaaatccc
    16621 cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg agggttaatg
    16681 cttatttata aattactgct gttccatgga gggtattcgg accggttaca gaagtaatac
    16741 ggaaagattt tgcgcccatc tcatccgctt tttgcgccag ctgttcttcc agcgatccca
    16801 gatttgtccc cgcgttagca ctgattgtac cgactttttg ttggccttct ggcgttgact
    16861 gaacttcgac agccgcaaag ctggcaaatg acatggagct taaaatcgcc gcagcgatga
    16921 gggtttttac gtttttcata atagtggcct tatgcagatg aatgacgtcg caagcaatta
    16981 cttaacgatc gataactaaa tgatagatgt ga
//
LOCUS       DAZUCE010000104        16887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZUCE010000104 DAZUCE010000000
VERSION     DAZUCE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.104"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(152..724)
                     /locus_tag="ACHY03_002836"
     CDS             complement(152..724)
                     /locus_tag="ACHY03_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906044.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAVTKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYIT
                     YASPAPGHLKDKFIEPLVKRVKDVAEGRVR"
     gene            916..1091
                     /locus_tag="ACHY03_002837"
                     /pseudo
     CDS             916..1091
                     /locus_tag="ACHY03_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1137..1400)
                     /locus_tag="ACHY03_002838"
     CDS             complement(1137..1400)
                     /locus_tag="ACHY03_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS3906045.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
     gene            1590..1829
                     /locus_tag="ACHY03_002839"
     CDS             1590..1829
                     /locus_tag="ACHY03_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906046.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPDFTPAQDKPQ
                     NMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
     gene            complement(1880..3376)
                     /locus_tag="ACHY03_002840"
     CDS             complement(1880..3376)
                     /locus_tag="ACHY03_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFS3906047.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEVSD
                     HYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAV
                     ARIAGTAGSAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLIT
                     LTFVVFAVHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGT
                     MFLVLPAVWLGALSWAGVSVGTVMGDAINKGTSNVSKAAAKSGDLANK"
     gene            complement(3383..3724)
                     /locus_tag="ACHY03_002841"
     CDS             complement(3383..3724)
                     /locus_tag="ACHY03_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906048.1"
                     /translation="MSRIYGRVKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            complement(3729..5150)
                     /locus_tag="ACHY03_002842"
     CDS             complement(3729..5150)
                     /locus_tag="ACHY03_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFS3906049.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
     gene            complement(5164..6138)
                     /locus_tag="ACHY03_002843"
     CDS             complement(5164..6138)
                     /locus_tag="ACHY03_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFS3906050.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWLFCTKFSCKVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGYWPPDPVEENTGTRNHKWQRL
                     TPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            complement(6135..6530)
                     /locus_tag="ACHY03_002844"
     CDS             complement(6135..6530)
                     /locus_tag="ACHY03_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFS3906051.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNPPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            complement(6666..7298)
                     /locus_tag="ACHY03_002845"
     CDS             complement(6666..7298)
                     /locus_tag="ACHY03_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS3906052.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSSVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            complement(7490..7768)
                     /locus_tag="ACHY03_002846"
     CDS             complement(7490..7768)
                     /locus_tag="ACHY03_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906053.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPEKGAEISVTEE"
     gene            complement(8148..8336)
                     /locus_tag="ACHY03_002847"
     CDS             complement(8148..8336)
                     /locus_tag="ACHY03_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFS3906054.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQDECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            complement(8714..9091)
                     /locus_tag="ACHY03_002848"
     CDS             complement(8714..9091)
                     /locus_tag="ACHY03_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS3906055.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            complement(9096..11792)
                     /locus_tag="ACHY03_002849"
     CDS             complement(9096..11792)
                     /locus_tag="ACHY03_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFS3906056.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRAREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLAGRE"
     gene            complement(11930..12322)
                     /locus_tag="ACHY03_002850"
     CDS             complement(11930..12322)
                     /locus_tag="ACHY03_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFS3906057.1"
                     /translation="MTVMVAVFILPGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            complement(12358..13845)
                     /locus_tag="ACHY03_002851"
     CDS             complement(12358..13845)
                     /locus_tag="ACHY03_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFS3906058.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            complement(13835..14743)
                     /locus_tag="ACHY03_002852"
     CDS             complement(13835..14743)
                     /locus_tag="ACHY03_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFS3906059.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            complement(14743..15405)
                     /locus_tag="ACHY03_002853"
     CDS             complement(14743..15405)
                     /locus_tag="ACHY03_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFS3906060.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            complement(15402..15764)
                     /locus_tag="ACHY03_002854"
     CDS             complement(15402..15764)
                     /locus_tag="ACHY03_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFS3906061.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGGIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            complement(15777..16091)
                     /locus_tag="ACHY03_002855"
     CDS             complement(15777..16091)
                     /locus_tag="ACHY03_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFS3906062.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            complement(16168..16410)
                     /locus_tag="ACHY03_002856"
     CDS             complement(16168..16410)
                     /locus_tag="ACHY03_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFS3906063.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            complement(16410..16751)
                     /locus_tag="ACHY03_002857"
     CDS             complement(16410..16751)
                     /locus_tag="ACHY03_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFS3906064.1"
                     /translation="MFSFPASPCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
BASE COUNT         3928 a         5313 c         3785 g         3861 t
ORIGIN      
        1 tgatatgccg tcagggtgtg cttccttaca tatcacgccc tcttccagac gtcgctggtc
       61 gtcgccctct gcgtccctta ctttcagccc ctctgagcat aatcgcccct ccagaggagc
      121 ccatcacaac gacaatgcca gcgggttaat tttatctgac ccggccttca gccacatcct
      181 tcacacgttt aactaatggc tcaataaatt tatctttcag atgccccggt gccggagatg
      241 catacgttat atatatccga cttcctttgc catttttttc aagttcaagc gtcagatgat
      301 catcaaactc gccaccatcc cagtcactgc ccattttata ataactaccg ggcatggcat
      361 cccacatatg cccgttctga cttattgctg ctgcggccca cttcccgttc gcatgtccgt
      421 tattacgaat cgcatcgact tcttcatttg aataaaactt atagtaccgt ttcagtcttc
      481 ccgcagctgt atccacatca acagggactt ctatgacatg atcacgtttc tctctttttg
      541 cccggggtgc ctgagacaga tatttgagat catcatctgc ctggtttaca cctacgttca
      601 gtacttttgt cacagcaaat gcaccgtcac ttatctgcct gttcagctct gcaatttcct
      661 gtgcagaaca ggcactcagc gcaacacacg aagcacaaac gagtaacatt tttttagtca
      721 gcatgtcttc atcctcatca gcagctcttc agtggataac atccacagtt acacctgcat
      781 ttacgcgcaa caaaaacacc agatgaagat tttattcatt cagctccgct aaaaattaat
      841 cttgctgtgg aatatcggga aaacaggaag ttacgttaaa agccgaagga aaaactcaca
      901 atcccttctt caatctacat cagtatcatg cccaaaccag acgggttaac ggcagcaaag
      961 aaccttgcag aagcgttcga gcattataac gaatggcatc cacatagtgc gctgggttat
     1021 cgctctccac gggaatatct gcggcggcgg gcagtaatgg gttaagtgat accagatgtc
     1081 tggaaatata ggagcaaatc caccacgcat ggagcctgtt aagactctca ctctcatcat
     1141 tctcccttct gagtcccaac gaaacgacca aaacgctgag taaagcgggc aacattgcct
     1201 tctgatgcca ccgttctgag tttaccggta tagaacggat gcgatttgga ggaaacgtca
     1261 atcgtcacat acggatacgt cacaccatcc agttcaatct cgcgatctgt tttgatggta
     1321 gaacctattt taaagtactc atccacactc gtgtcatgga acactactgt gcggtattca
     1381 ggatggatat ttggcttcat ctctcttcac tcatgttata acataacaat aagatacatt
     1441 ctcagctaga tcatttcaac agaatccccg agcccaaggg taatgtggca tcgcagcctc
     1501 tcatcaaatg gaagacatgt gattgcacat ttatcataca agacgtatga taacactgtg
     1561 ttatttatcc tgctgctgag ggtatagata tgaataacga atctgaaaaa gcaatgtggc
     1621 gttgtaagcc tgtggataat ccatggacct tcacttccct gctgaaaaaa gcagtctgtg
     1681 ccgttttacc agatttcaca cctgctcaag ataaacccca aaatatgttt tcactaagta
     1741 atgaaaaaga tactgattat tcagtaccaa tgagaccctt tgcattatat gacccgtgga
     1801 aacatcacca tgacaatgct gatttctaag aaaagcaagg gtttccccaa ttatcccagc
     1861 taaggaaacc ctaagaattt tacttattag ctaaatctcc actttttgct gctgccttag
     1921 atacattact tgttcctttg ttaattgcat cccccataac cgttccgaca cttaccccag
     1981 cccaggacaa cgctcccagc cacactgccg gcaagacgag aaacatcgtc cccatcacca
     2041 gattcattat cagatcatcc gatgtgttct ggaatcccat catattgaac cgggtatgtg
     2101 tatccgaact gtacagcgcc gtcagcatcc agctgtcaag ccaccgtgca agctcccacc
     2161 agaatgtcag aaagtgtacc gcaaacacca caaacgtcag tgtgattaac gtcgacagtt
     2221 catatgccgc aaacgccaga atcagcggta tcatgatgta aatcgccatc agtattatcg
     2281 actgtaccat cggcagtgcc tgccgcattg catccagtgc cggaaacgca cccagtgctg
     2341 ctaccgcact gcccgccgtt cccgcaatcc gggccaccgc attcatggtc gtcatatctg
     2401 cattgccacc atagccacga tacacctctc ccccctgaga gagcgtcagg tttgccggac
     2461 tcaccagacg cttgatcacc tcctccttat aatccacctg actactgcca atcatcttca
     2521 ttgcagctga cagacgtaac cagtaatgat cactgacctc ctccatgacc cgttttttca
     2581 gccccacact actgtcactc caccactccc ggcatgtcgg atagcctccc cgccccgtat
     2641 ccggtcgtcc gctgtcacgt gctgtattcc acgggaacgc agcccgcggc atctttgact
     2701 gcagcgtccc gtaatccccc tgcatgaatg tggcgctccc tatccaggag atatcatcaa
     2761 gcactttttt atctttactc tgcccctgat cacgctgttt ccacatatac agtgccagtg
     2821 aataacagtc gttcgtaaag tcctgcagct catcagcaag tgccctgttt tctataaacg
     2881 ttcgctggat ctcaaaacgc aactggcgca aatcaggccg gcacggaatc gaagccactg
     2941 ccgcctgggt gacacctttc gacaatttat gaaccaccac ccaccatacc ggggcagcag
     3001 cagtctgatc ccctatactg ctgatcaccc cactgtaccc cgtctctccg ggctcttcag
     3061 gtgtccatac cccacaggtc tgtgccctgg atttatcgaa tgacagcgta tccagcgtca
     3121 cctgtaccag aggaacacag caggatatca tcacaaaaaa agcaccatag agaacatgct
     3181 caatacgcgg cagggaaagc atccctttgt ttccctcatc cgcaccttct tccctgactt
     3241 tcagccagat cccgatgatc ctgaatgcca gtggcagggc aaacagtcct gtgctgatca
     3301 acacattcca cagaccgtta ttcaccaccc agcccagcag agtcagaaaa tattccagat
     3361 agttatttgt ggtcatgatt gctcaacgcc ccggctcagc aaaaatggcg tattcacaca
     3421 gcaggataaa cagaacactg actgtggcca tacgcagaaa agaagcccgg tattctgccc
     3481 tgaaccccgg cgcatgccag atcctccacc ccagccatcc gatcgcactg taaagacaca
     3541 gacgccatcc cagccagcca taacgtgtct catacatcca ctcccgcagg gccgcaaatc
     3601 cttctgcgtg acgtataccg gtatcggcca gccagaggga aaacacggta atccctgtca
     3661 gcaacagcgt atacagggcg aataccttca tcctcttttt cacccggcca taaattctgc
     3721 tcatcctctc actccttatc cggggcatcg agagaatgga tacggctgtc agcattgtca
     3781 gcctgctgga tcatcgggtt attccccaca cgctcgttat cccgttcaat gatggtcaga
     3841 acactgttac gcgccagatc ccgcttcagc tccatctcgt ttttcagcgc cataatttcc
     3901 cgatccagcg cctcaatacg gcgttccccc tcttccagtg cagcaggctg agctgcagca
     3961 tacggctcgg ccatccccgt gagcagcatc ctgcgcatca gcaacgccgt ttcaacggta
     4021 tccgccatgg ccagttctcc cgccagacgc gacgtcaggg caccattgtc cggatcacgc
     4081 tgcagggcac ggatcactcc cgctgtcaca gacagactcc cggctttcag ttttgccaga
     4141 ttcccggctg tcggcttgtc acgaccattg accatacctg ccagcagttc cgtgttaacc
     4201 cgcgtcgctt cttcaagcat cggcgcaaac cccgtgccgg caatactggt ccccggctgc
     4261 tgatcagcat ccccgctggt acatgccttt gcatcaccac aggtccgcat ggatttatcc
     4321 cccagaacct tcaccaccgc ccgggcagct tcctctgcat tacggtattt catgcaggca
     4381 ctcccgttac agttcccggg actgacactg gatgtactgg tcgaaggcag actgttcatc
     4441 atattgaaac cggcagctgt cagatcatgt gtcggccgga tagcagactg tcccgctcca
     4501 ccacgctgct gccctccgat ccagcgttgc ccctcctccc cggtaacctg acgacctgct
     4561 tcatcccctt tgatcgcatc accgtccccc gtattcacaa tttttttgta ctcctcgagc
     4621 atggccgcct gcgtccactt attgctgtcc gtgaactcca tcatgcgttt cgccatgttc
     4681 tgacaattga gctgcgcttt gtcgaatgcc acattcgcct gcagtacacc gttggtcagc
     4741 atgtcataca gccccggatt agcccgctgt atcaccattg ccggcagact cgccacggct
     4801 ccgctcgccc cctgaatcac atcccccatc aggtttttga aaccatcagt gatcccgttc
     4861 agctggttac ccactgttgt tttcaggtca aaattgccac acatcagatc actgctccac
     4921 ccgagatcca ccccaagttt ctgcatattg ctgcgcgttg ccggctgcga tatcaccgag
     4981 ccccctccca gggtataaaa cagtttgtct gatactgcgc cgtccacgga tttaccgtag
     5041 ccaaacgcac tgtcattcac tttcggcagg gacaaaaagt ttccgtcagc ctgtaccgta
     5101 cccgcacaaa gcaccacagc cagaacaggt atcgtcattt tctgtttcat cacttcccct
     5161 tacttaaaaa ttcgtactgc ccaggaatgt ctgtccccgt ttcttgcagc aactgtaggg
     5221 ctgccacagg gcaaaggcag cattacctgt ttcagccgct tcatgctctc cgtcagggaa
     5281 gactgcgcag gacgtggaga ctgaaggcgt cagtctctgc catttatggt tccgggtgcc
     5341 cgtattttcc tccaccggat ccggtggcca gtaaccgtcc ttcttccctg ctttcaccgg
     5401 ctgatagaca tgtggttcac cggtgcgggt gataatgtca gccacccgct gggccaccac
     5461 cgcagcagct ttatcatcat ctgtctgggt cacgaacccg gagcggggat acacattgcc
     5521 ccacatattc tcttcttcct ggctgcctat ttcgcgttta ccggggatca gggcctccgg
     5581 gtacagtgac tccggtagtc ctgtacgcca ggcaatactg tcaagcgtac tgagaaaata
     5641 aggaacaaaa ggtgtcaccg gtgactcaca ggaatatccc ggcaccatcc ctccgataag
     5701 cgtggtggcc gggtgccctg ttgcatcggc atatttaaaa cggattgacg aacgacgttc
     5761 cccctgtacc ttcatgtctg cggggttccc tcctcctgct gacagaccgg caataccttt
     5821 gcttatggca ttctccggac caccggctgt actgctgacc gccgacattt cacgccaggg
     5881 atttccgcca ggtgcggtat aggtggaaac aaccacttcc gggatataat gggaaacttt
     5941 cactgatgtt ctgaccttgc aactgaattt ggtgcagaac agccagtagc aaatcccgct
     6001 gacccgccag ctgatacagc tctgtgaggc tgcactggcc actatctgcg cggtgttcag
     6061 cgatgcctct gccaccggca ataccgccat acttaacagc agaacaccgg cgcgatgacg
     6121 tgatgtgcag gaaatcatgg ctgccctccc cctgtacgca gttgttctgc cagcaccacg
     6181 tctgtggttc cgtagaccac ttcactgtca tcaaaaacca cagccggata ttttttcagt
     6241 ccaagggacc aggcatgtgc taatgcacgg tagtgttcag caagccctgc ctgaaacgac
     6301 tgccattcag gcgactgcat ccgttccatg acacgggcag atgcttctgc aggctctgcg
     6361 ggaagtaccc cgaaaaatgt atcctgcgtc tgctcaggag catcaagcag gacaacacga
     6421 acacctgctg gcgggttcat ggggcgctgg tgcgaatcgg tataaattgt tgtaccggca
     6481 gatgcagcca ccggtagtat caggaaggaa tacagtaaca gtcggtacat gatgcgactc
     6541 cgtcagacaa ctggtatctg acagcgtgcg caaaggcaaa gggacaatca gcaataaatt
     6601 caaaaccgga tgataaaaat taagcctctt cctctgaata acgcacactc tgcatctgaa
     6661 tcaaattaat ccggttgcat cacaggaagc cactgattcg gggtaatacc ataataacgc
     6721 ctgaacacag aaataaaata agaagcactg gcatagccac acaactctgc cacccgggaa
     6781 atacggtttt ctcccgcaga caaaagctga gcggcatact gcattctctc tttcagaaga
     6841 agctcactga aggtcgtcag ctcttcctga agctttttct tcagtaaact ctcacttata
     6901 tataacagac tggccacttc acccagcatc catggacggg acaaatcagc actgataata
     6961 ttcttcaccc ggacagtgca gctccggata caccccgtga gcagggcgtg gaaatccgga
     7021 tgttcctcaa acagcgccag actggaaaaa gccaccgcat ccctgaagta caccgacgat
     7081 ggttcatctg taataagttg tctgatcaga cattccgcca gcacggacat ctgtggccgg
     7141 atctgtaaaa gcaaagacga cgggctgact gccataccgg ataatggcgt gcagaaccgc
     7201 agataccgcc tcaccacctc ctcactgagg tcagcgataa ggaaacgtcc ggtatgagcc
     7261 tgaaaagcag ggcgcagatg cccggcaatc agaagcattg tgccggcagg tatgacagag
     7321 ttcctgtgct ccagacaaag cattgtctgt ttgagagcca taatgacaac gcaggtacag
     7381 ggcatcggat atccggtaaa tatgttgatt tattaaagag acagacatgc cccgtatata
     7441 aaattccgtg tcaccgggtg ctgaaaaaaa ctgcaccgac ggcgctcttt tactcctccg
     7501 ttacggatat ttcagccccc ttttcaggag cggtatcctt cacctcgctg agcccggact
     7561 gagccgcttc agaaacctct gccacctttc cggcagcctg cattttcact tcctccgcgg
     7621 catccttcac atcactgagt tgagtctgcg cagcttcctt tatatctgcc gccttcttac
     7681 ttacattctc ctttgtcgca tcatcacagc ccgcaaccag aaatggtgcg gaaatacata
     7741 acgccagaat tgttttttca gcatacaatc tctcattatt aagtgttaac gcagaacagc
     7801 ttatatttcg tccccgggaa atggtcaaaa gacaaccaga acatcacagt gtcagggcac
     7861 actcccggta tatgctttta taaccataac atgctgttcc tgatattacc ggaaatgtgc
     7921 ccactacatt taacctgttt tcagcaccgg gtcatcggct gtgctgtatt ttttattccc
     7981 gtcacagaaa cacactatta ccgttgattt catgacgaat aaaatgaata aattctcccg
     8041 tggtctttac caccattatt ataccaataa caccatttac acagatacac tccgggacta
     8101 tctgtttttg ccccctccga ctccgccgga ggtttttatt ttcatactca ggccttcacc
     8161 acttcacacc ggaagtcctc caccctgacg ccaatatacc gtcccggctc ttttgcgggc
     8221 gcaaacagtt tttcacattc atcctgagtc attttttcat cctggtaacg cagccactga
     8281 accggcatat tgcctggttt gattattgtc gcagttattt tatatcgctt tcccatactg
     8341 gctctcatct caacaacaaa atcaccggct gagccatcac tccggtaatg catgcgaaaa
     8401 aaagtgagcg tctcagcaat ggctcatgcg gtgattctgt acggtacccg cacaaaatac
     8461 gggcaccaac agcgtcgcag aatacaacga aggcatcagg gcagcacctc tctcccgcta
     8521 tttttcagag ttctttaccc tcctcctgac acgggagtac aaccctgaca gcatccgggc
     8581 cagtacattc gcaagccccc atgaaaacac agccacaccc acagctcccg ccagaaacag
     8641 cacaggttca ctggcaattt gatctgacag cgatggatac agtggtcccg gaaaatcgat
     8701 atcgctcctc atatcacccc tctcccagcc acaacagcag atagaacaaa tccgccgcat
     8761 cagtcactga tatataccag cggggatgaa tatactcctc ctccgacggc cagctctcat
     8821 gcccactcac agtaagcaga atatgcacat actgaccctg ctgaccttcc agcatcaccg
     8881 ccacactgct gttcagaccc accctctgtg gctggcaacg gatacactcc acccgcccca
     8941 gttgcgccgc cagatgcccg cgaagacgtt caagattatc ccgtgtggtg caacttatcc
     9001 ccggcgcacc ctgtggcggc agaacaaaca cacttccggc ggggtgaaca gcagacacca
     9061 cttccggttc cggatacttc agacacagca tagtctcact ccctgccagc cagaccacgt
     9121 agcccgtcca gctttcgtgc cacatgaaac gctgcttcca gttcgctgca ctggtgcacc
     9181 ttcatcaggg cgcggcgttc agccttctct tctttctccg tcatccccag agccagatac
     9241 agactcggtg gcacaacccg gaacaacgct tctgtgcgct tcgccagtac caccccctcc
     9301 gtataacagc cggacagttt actggctgac agcagtaccg ccttctgctc ctccgtcagg
     9361 gatttaaaac gggcaatatt gttcacttcc tccggcggca tggtcaggca cacccaccac
     9421 tctgccatat tcagcatctt ctccgagaca tccggataat ccttcaggtt ctgcgtggcc
     9481 agccacagcc aggcccccag cttgcgccac attttcacca ctttggtcat atacggggac
     9541 aaaagcgggt ttaccgtcac gatatgcgcc tcatccacca caaacagaat atcccgtccc
     9601 agatactgat cgcgctcggc aatgttgtta atcatattag tcattgacac catggtcagt
     9661 gccatctggg cttcataacc ttcacgggca agatgtccca gatcaatcag ggtcacatcc
     9721 gcttccggcc acagttttcc ttcccggtta aacagctccg cttcaaaact gcccggctgg
     9781 gtgaacatcc ccagcgactc tgccatctca gcagctttag cccggcgaac atcattacgc
     9841 tgagtgtccc gcgaaatttc ccacaacgca ctctgcagat ccaccggcag catctgacgc
     9901 ccctcccggt acgtggtgtg cgtggccatc agcagtgcct cacggatcat cgccctgtcc
     9961 gcacgtttga gcgcggcttc ttctttcgga tcccctccgg tgatcatcat gcgggcagag
    10021 atttccatct cccccagaat gtcgcgtttc tcttcctctt catccccctc atcttcatca
    10081 agatcgggca gtgaatgctc atccacactc tgcagcgtct ctccctgctc caccagttga
    10141 tgagcatccg caaatggagg aagacacacc cccgttcccg gtttgacact gattttatta
    10201 accgaaagcc cgagactctc aaaataatcc gccagcagac caaacgagtt cccggcctca
    10261 gcgataaaca gacggggacg atgaactgcc attatctgcg acaacgaacc acacagcgtg
    10321 gccgatttac cggctccggt aggcccgaaa agcagcagat gtgcattctg ggtccggtcc
    10381 agcttgttca gcggatcaaa ggtcagcaca tccccgccac ggttaaagaa actgaatccg
    10441 ggatgccccg taccggtttc ccttcccgtc accggcagga gccctgccag atgctgtacc
    10501 cacatcagac gcgtgtacca ctgtttttta tccgtatcag gatcaaaaca catgggcagc
    10561 gcgcgcagcc agctgttcag cggtccgaca tcaaactccg ggcgcactgg ctgtaagccc
    10621 gcgcccagca gcaccgtcgt cagttcaacc cgtctgcgct tcagactctc catatcctct
    10681 gcccgcagca gaaacgtgat cccggcacgg tagagcttgt gccggtttcc cagatatttc
    10741 ttcacggtcg ccacatcctc ccgggcacgc agggattcac tgttctcccc gaccgcattt
    10801 ttcgacaacc tcgtgaagcg ctcttccagt gtgtcctgtg gctggaccac aattgtcata
    10861 cacagcaccg ttccctccgg aaaggtgtcc atcagggcat tcactgtctt ttcgccccgg
    10921 gcctgttccc ctgtgatggt tcccggctcc ggtggtgtgc gcagtttctc caccacaaca
    10981 gcggcatgcg gaagcccgtc cagccaccac acgccgtttt ccggatcaga taccggtggg
    11041 gtgaaccaga ggctttctgc aaaatcggtc atcaccggca tcattccctc cggtgtctcc
    11101 tgcggttctg cacgggatgc catacggtac agaatatccc tgtccaccca ttccggccgg
    11161 ggattgaaca gcctcagcag ccagccatgc acctgctgcc cgttcatccg tgtacagcgg
    11221 acgcctgcgc cacccagcgc ccccacgacc cgcgaacaga cctggttcag cattgccacc
    11281 ggcggcatcg gatcgtggtt gtttttcccg atccaccggt aaatcaccat cctcgtgcgc
    11341 cgctgctgac cacgccatgg ctgacctgtg accagcgtat ccctgaacag accttccggt
    11401 cgggcaatcc ccttcagatg gcgctccatt tccctcagcc aggcctccgt aaatgaggag
    11461 ccttctgcat gaggtttcac gtaccccctg agctggtcaa catacgtgtc cacgtcgttt
    11521 tcatcctgac agaagaactg caccacccag ggatgtgagt catattcatc aaaactgtcc
    11581 tgcagcgcat cctccaccgt atcccggatt tgctccagac gctctgcagt ccgcccttcc
    11641 gtagcggccg gactcacctc atacacggca ccaaccgaca ccccatcatc cagcagcagg
    11701 cactgctctt cgtccagata ttccacccat ggcagaaaat caatcaccga cggacaggca
    11761 tggtaaacct tcgtctcatc ctgtcgggtc atcttccccg gccttgacag tgcctttcgt
    11821 acctgagggg ccactctttc tttcatatcc ggagataccg cttctccgtc atgctgcgac
    11881 accggggaag aaacccgttt ttttgtgtct gtaaacaggg aaaatcgcat cacaacgcct
    11941 ccgttcgttc acccggcatg gcatactgcg tccggctgta aaaaggaaat actgtgctgt
    12001 accccggaac cggtgacgta ccggaagcca gatgcggaaa aacatacatc accatgtcag
    12061 gattgggcag ccgcggaaac tgctgactga tctcactttc ctgtgtccgg ctgtagctta
    12121 tggctccgtg cgcatctgcc tgctgctccg gcattgtcag cggacgccgc agggtgtccc
    12181 tggccgctgt catgtgccgc gaatgtcctc cgcttccttt cccctgccag atctccagca
    12241 tactctgttc acccggaggc agaagctcct ctttcgatgt cgagcagccg ggcagaataa
    12301 ataccgccac catgacggtc attacagata actgtcgttt cacgggatct ccttacatca
    12361 gtccagacca ccggtaccgt ggacttcccc cggcagagtg aagtcgtaac gtacccggcg
    12421 cccctgatct tcataatcaa tggccagttg acgcgtgata tgcaccgcca gcgtctgccc
    12481 cgggggaaca taaatggcat caaacgtctg cccgtatcgc tgcttcaccc attccgcggt
    12541 ttcattcatc ccgccggaca gggcttttcc aagtgccgcc tctccggcac tgccggtcag
    12601 tgttgatgtg atgccattga cgttgttctg tgaggtgtac tgcccctcac tcagtgcatc
    12661 acccgcagcg ccggctgcag acagggcaaa tattgtcggc agatacgtgg aggcattgga
    12721 tttacgggtt ccgctgatac acgggatccc gtcatcatcc gaaatccagc caatgcctcc
    12781 tgtactgctg ttattttttg cctgtgcgtt actgctgccg ttcacgtctg atgacggcag
    12841 cgtacgcact gtcccgtcgg caaaaacaaa cgtgatgctg ttcacctgcc cacggacaca
    12901 ggagagcgtc cagtcaccac tcgcagtacc ggacaccact gcaccttcca catccggcag
    12961 ctctattccg tttgccgtca ggttatcctt acccaccagg atcttgaacg gatagggatc
    13021 cgtcacggtt ccgttcaccg gaacccgtcc cagaagcgct gtcatcgccc ggctgcctgc
    13081 cagcgtcgag ttctccggca gggtatacac tgcccgggtg ctcgccttct gctgttcatt
    13141 gctgtatccc ttcacctgct gttcataagc cgctttctga cgggtcagtg gattgtcatc
    13201 cagaaaagag actggaaaac gcggtccgct cttatccccc accgacgacg cttcatcctg
    13261 cacctgatcg gccggtgtca cccacatcag accatccgtt cccggtgagg ctgccgatcc
    13321 tgtcacgctc cccagtccgt caaggcccag acctgctgga atatcgctct cctgtcctcc
    13381 ggcgccggca tcagcactct cctgctgacg cttcagttgc tctgtcagtg actgaacctg
    13441 tgtcattaac gcgttctgtt cattccggaa ctggcgctgc gactgctcat aatttttctg
    13501 caatcctgcc actgcctcac tgacctgacc ggaaacatcc gtgccccgct tgcgcagcat
    13561 ttcattttct ttcagcaact tttcattctg tttgtccagt accagttgcc gctccctcac
    13621 ctgattcata cgcccaatca gtgtgcgcag ggtatcttca ggtgtgtctc cttccacgcc
    13681 cagggccttc agttcatccg gtgacagtcc ggccagcgtt cctgagcttt ccgcagacga
    13741 gggctccccg gtatctcctc cacagctttt taatcccgtc aggatccccg ccaccaccac
    13801 acagggcacc acatacttca cgatcgtatt ggatttaatc tgcatgcttc acctcccccc
    13861 gtgaaggaat gcgacgggag gcaggctccg cgataaacgc ctgttccggc cgttctgccg
    13921 tcaccacata gagcgtggtg gtatcctcag gggtacctgc tgcacccagc cagcggtgct
    13981 ggaaagtcgc ggcaatgaat tttccctgaa gtgcccgcgg atcaaggata attttgcggc
    14041 ttaccgtact cgtcagtttc agtgcaacaa ctgcacgccc acccgcagcc caggctgcca
    14101 gtggcgtgac tgtcagtggc tctgacggat agagtgtggt gattttcccg ggcagatgtg
    14161 tattaaccgg ccggataccc ggtaccgctt ccagcgtccg taacggtgcg tacagcgact
    14221 gtgccgcata gcgggtcaac agaacgggta aaggctctgc atacttcacc ggctgacgga
    14281 gggcctgcga gccgtcatcc ccgccgggta ccaccttttt gtgttctgca gagcgtgcag
    14341 acgccgctgt gccagcctga ctgaccgtac tgacgcttcc ctcatacaca atccgtacgg
    14401 gctctgtggg ccctttttct gctgctgtca catccagcag aataatttct ccgctctcca
    14461 catcctgcag ttgcagacgc gtctgcggaa aaacactctc tgctttcaga tagactgccc
    14521 caccggtact ctgtaccctc aactttccgt tcagtgctgg cgggaagccc acccgcacat
    14581 ttttatctgc aaaaataacc cgctcctgtc ctgccatcag ggtaacgggt aagggaatgc
    14641 gctcccattt catcagctca tcagcccgtg cgctcagtgg taaaaccagc agtgacagga
    14701 ccatcacaat actacggtat aaacaacgtt cagctccgtt catcactgat tatccccctt
    14761 cccggctgcc ggggaggctg cttccagccg ctgtggtgta ctgctgtagc agtccagcgc
    14821 cagcccgaac ggattacgtt caggatcccc ctccatgcgc accaccttta tcggataacg
    14881 gacaagtgcc cgttttaccg gctcagcatg aaaatactca tctgccacca gatccagctg
    14941 tacctgccag ttatcccgcc cttccaccgt caccggattt ttgttgtacc tgcccccgta
    15001 gccatgttcc ggaatttcat acaccacgcg cacacgctcc agcagctccc ccgcatcact
    15061 gcgacggcgg gcatcactct ccagaatctc ccgacaggat ggcgtcaggt aggcagagag
    15121 cgcatgaatt ttcgccgggt agtccacctc tccgttcttc ggccaggcat tcagttgctg
    15181 aaagatataa aaggcaaagg aataaaccgt tgacggcggg acttcccacc agggacgggt
    15241 actgccggaa cgcagatccg gcggattgtg aattgtcagt ctgtccggtg ccgtcatcca
    15301 cccgatacag accagcatca gaattccggc cagcacaccg ctgaaaatac gcagtgtcag
    15361 gatatgctgg tcgcgatccc gcagagcatg acgaaaacga ctcatttttt ccccctgcta
    15421 cgggtacggc gcagggacca gccatgcgcc tcccggataa atgttttccg gccaccaccc
    15481 catgcactgc ttttcttcgc cagatgctgc cagagccagt tgtcaggttt tccccgcttc
    15541 agccggctga tccagacgcc accgaaccag accagcagca ggggagtcag caacatccct
    15601 gtcgggataa tcacccagcc aagtagagga atcagcggta acggccagat cccccctgcc
    15661 acagcgcctg tcagggccgc cagccccagt tccggtgtgg taaaaccacg gaaaaccgca
    15721 ggctccacgt tcagacgatc gggcagaaaa cgaatggtct gcatggggat gtctccttac
    15781 agaatgatgg aggccgactt gcccagcagc cagataatgg cgaccagcag aacaacacca
    15841 accaccacga tggcaccaaa ctgcccccat ccggccttcc cgtcacgaac ctctgagaag
    15901 gtggaaagcg ccgcttccgc caccttcaca aaagcaactg cactgagcac aagaccaaac
    15961 agcacaacac catcctggag atagcctttg atctgtccga aaagtccacc acctccgccg
    16021 gatgttggcg gctctatctg aggcaaatca gccagagcag gtgtgatccc ggtacacaga
    16081 aacaccgcca tggcatacca ggcctggcga cagatgcgca tttttttatg cattatttcc
    16141 tcctgaaact taaacaagac actgaaatca acccgcaaac atccagatac tgcagatcag
    16201 cagcaggacg agtcggaaca taaaccagcc gaacacacca tcccggacat caccgctacg
    16261 ccagcctgcc caggcatccg tcagactcca cgctgcccag aggaacacaa ctgccagcag
    16321 aaatcccagc gtgatgagat gcagcacact gagatccact tcacctgaag cagcccggaa
    16381 ggccgtattc tgcagggccg tcatcgccat cagggacgct cccgccggta gtcaccggtc
    16441 acggctgcgg aagaccgggg ttgcgcccgg gaaggggcca gataatggtc aataccgtca
    16501 cgaatggtct gcagatctgc acgggcacgc agataatcaa aaaagtagcg ttccccctgt
    16561 gaagaagcct gcagagaggc cgttctggcc cgctccagcg ctgcctgcac gtggtcaagc
    16621 tggcgcatta cagtggccag ttcatccttt tcggcagcct gcgcaggtaa tgcaaaggac
    16681 aaaagaaaca gcatccccgc ggcaccggcc agacggccgg cacggcaggg tgatgccgga
    16741 aaagaaaaca tacgcacctc cgtcagagtt aaacagtgcg tacaggatgc gtaaggcaga
    16801 ggactggatc agcgataaac tctatttgca aaacagaaaa tttttacggg agaatacact
    16861 ggtgtggatg aaggcaaaca acccggg
//
LOCUS       DAZUCE010000105        16820 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZUCE010000105 DAZUCE010000000
VERSION     DAZUCE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.105"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(341..1156)
                     /gene="djlA"
                     /locus_tag="ACHY03_002858"
     CDS             complement(341..1156)
                     /gene="djlA"
                     /locus_tag="ACHY03_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFS3906065.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1411..3765
                     /gene="lptD"
                     /locus_tag="ACHY03_002859"
     CDS             1411..3765
                     /gene="lptD"
                     /locus_tag="ACHY03_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFS3906066.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            3818..5104
                     /gene="surA"
                     /locus_tag="ACHY03_002860"
     CDS             3818..5104
                     /gene="surA"
                     /locus_tag="ACHY03_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFS3906067.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            5104..6093
                     /gene="pdxA"
                     /locus_tag="ACHY03_002861"
     CDS             5104..6093
                     /gene="pdxA"
                     /locus_tag="ACHY03_002861"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFS3906068.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRSYSPNSPAQPQTAGTLTLLPVALRAPVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            6090..6911
                     /gene="rsmA"
                     /locus_tag="ACHY03_002862"
     CDS             6090..6911
                     /gene="rsmA"
                     /locus_tag="ACHY03_002862"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFS3906069.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            6914..7291
                     /gene="apaG"
                     /locus_tag="ACHY03_002863"
     CDS             6914..7291
                     /gene="apaG"
                     /locus_tag="ACHY03_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFS3906070.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            7298..8146
                     /gene="apaH"
                     /locus_tag="ACHY03_002864"
     CDS             7298..8146
                     /gene="apaH"
                     /locus_tag="ACHY03_002864"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFS3906071.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKHYFVQPSNRHKDMGE
                     GEAVAS"
     gene            complement(8143..8457)
                     /locus_tag="ACHY03_002865"
     CDS             complement(8143..8457)
                     /locus_tag="ACHY03_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFS3906072.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFI
                     LIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(8460..8693)
                     /locus_tag="ACHY03_002866"
     CDS             complement(8460..8693)
                     /locus_tag="ACHY03_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFS3906073.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(8779..9258)
                     /gene="folA"
                     /locus_tag="ACHY03_002867"
     CDS             complement(8779..9258)
                     /gene="folA"
                     /locus_tag="ACHY03_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFS3906074.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(9450..11312)
                     /gene="kefC"
                     /locus_tag="ACHY03_002868"
     CDS             complement(9450..11312)
                     /gene="kefC"
                     /locus_tag="ACHY03_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFS3906075.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(11305..11835)
                     /gene="kefF"
                     /locus_tag="ACHY03_002869"
     CDS             complement(11305..11835)
                     /gene="kefF"
                     /locus_tag="ACHY03_002869"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFS3906076.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(11946..13277)
                     /gene="yaaU"
                     /locus_tag="ACHY03_002870"
     CDS             complement(11946..13277)
                     /gene="yaaU"
                     /locus_tag="ACHY03_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3906077.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(13335..13622)
                     /gene="fixX"
                     /locus_tag="ACHY03_002871"
     CDS             complement(13335..13622)
                     /gene="fixX"
                     /locus_tag="ACHY03_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFS3906078.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(13619..14905)
                     /gene="fixC"
                     /locus_tag="ACHY03_002872"
     CDS             complement(13619..14905)
                     /gene="fixC"
                     /locus_tag="ACHY03_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS3906079.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(14956..15897)
                     /gene="fixB"
                     /locus_tag="ACHY03_002873"
     CDS             complement(14956..15897)
                     /gene="fixB"
                     /locus_tag="ACHY03_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFS3906080.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYASVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKDNIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(15912..16682)
                     /gene="fixA"
                     /locus_tag="ACHY03_002874"
     CDS             complement(15912..16682)
                     /gene="fixA"
                     /locus_tag="ACHY03_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFS3906081.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDV
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         4133 a         4672 c         4205 g         3810 t
ORIGIN      
        1 tcatatttaa aagtgtagtc ggcatgcctg gaactgatac tttcatggta atagcctcgc
       61 attgagttaa aaactcatga tgcgcactct gaataaatat actttcaaaa atcgctcatt
      121 cagatatgtg ttttttattt ttaagacgta aaaatataat tccgctcgtc gtaaagctct
      181 caaccttaag cagggtcaac gcgataaaac caacagaaac tctcccgtaa accgtgtatt
      241 gagcctgtcc accactctga gtaaacgcct tttctcaaag aagtgaccgc cagggcggcc
      301 acctttaatc gtgggcagtt actcagcatc atttacaggg tcacttaaac cctttctgct
      361 gcttaatcaa ttcataggcc tgctgaattt cctgcgcttt ttgcttcgcc atctccatca
      421 tctcaggcgg caaacctttc gccaccaact tatcgggatg gtgttcactc atcagcttac
      481 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccaaa acattacagg
      541 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
      601 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
      661 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
      721 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
      781 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattgtctg
      841 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
      901 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
      961 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
     1021 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
     1081 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
     1141 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
     1201 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
     1261 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
     1321 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
     1381 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
     1441 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
     1501 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
     1561 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
     1621 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
     1681 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
     1741 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
     1801 aaagatacca acgtctggga gggtgattac cagatggtgg gtcgccaggg ccgcggtaaa
     1861 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
     1921 acctcctgtc tgccgggttc tgacacctgg agtgtggtag gtagcgaaat tattcatgac
     1981 cgcgaagaac aagttgcgga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
     2041 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
     2101 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
     2161 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
     2221 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
     2281 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
     2341 tggttgttct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
     2401 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
     2461 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
     2521 accgtttcaa ccaagcagtt ccaggtcttc agcgaacaga acaccagtag ctactcggca
     2581 gagccgcagt tagacgttaa ctactaccag aatgatgttg gtccgtttga tacgcgtatt
     2641 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aactcgtgtt
     2701 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
     2761 gcgaagttgc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
     2821 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
     2881 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
     2941 cgcgcgcagt atttgtacgt gccatatcgc gatcagagcg acatctataa ctacgactca
     3001 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
     3061 cgtatagcct ccgctaacca ggtgacgacc ggtgtcacat ctcgcatata tgatgatgct
     3121 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
     3181 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
     3241 gatacttact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgatact
     3301 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
     3361 ctggtacagc tgaattaccg ttacgccagc ccggaatata ttcaggctac gctgcctaag
     3421 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
     3481 tggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
     3541 aagcaagctg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
     3601 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
     3661 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
     3721 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
     3781 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
     3841 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
     3901 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
     3961 ggtaaaactg aacgctgctc aggcaaggca gcaacttcct gatgacgcga cgctgcgcca
     4021 ccaaatcatg gaacgtttga tcatggatca aattatcctg cagatggggc agaaaatggg
     4081 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
     4141 catgacgctg gatcagatgc gcagccgtct ggcctacgat ggtctgaact acaacaccta
     4201 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
     4261 tcgcatcacc atcctgccgc aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
     4321 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
     4381 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
     4441 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
     4501 cggccagatg ggctggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
     4561 caccgcgaag aaaggcgaca ttgttggccc gattcgttca ggcgttggct tccatattct
     4621 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
     4681 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
     4741 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
     4801 ctcgcaggat ccaggttctg ctaaccaggg cggcgatctc ggctgggcta caccagatat
     4861 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
     4921 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
     4981 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
     5041 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
     5101 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
     5161 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
     5221 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgttcttat
     5281 tcccccaact cccctgcaca accgcaaact gcgggcacat taacgctact tcctgtcgcg
     5341 ctacgtgcac ctgtcactgc ggggcagtta gcggttgaaa atgggcatta cgtggtggag
     5401 acgctggcgc gagcgtgcga tggttgtctg aacggcgaat tcgctgcgct gatcacaggc
     5461 cccgtgcata aaggcgtcat taacgacgca ggcattccgt ttaccggtca taccgagttt
     5521 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
     5581 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgcg
     5641 cttttgcacg aagtgattgc tattttgcat cacgatttgc ggaccaaatt tggtattgcc
     5701 gaaccgcgca ttctggtctg cgggctgaat ccacacgcgg gcgaaggcgg tcatatgggt
     5761 acggaagaga tagacaccat tattccggtg ctcgacgagt tgcgggcgca ggggatgaaa
     5821 cttaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
     5881 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
     5941 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
     6001 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
     6061 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
     6121 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
     6181 ctgccattaa cccgcagaag ggccaggcga tggtcgaaat cggccccggt ctggcggcat
     6241 tgactgaacc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
     6301 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
     6361 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
     6421 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
     6481 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
     6541 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaatgtga
     6601 tcccggtact ggaagtaccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
     6661 tcgtgcgcct ggttcctcat gcaacgatgc ctcacccggt taaagatgtt cgcgtgttga
     6721 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
     6781 acctgtttag cgtcgaggtg ttaacaggaa tggggatcga cccggcgatg cgagcggaaa
     6841 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
     6901 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
     6961 tgaggctcaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
     7021 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ctaatggcaa
     7081 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
     7141 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
     7201 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
     7261 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
     7321 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccctgg
     7381 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggccccggtt cgctggatgt
     7441 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
     7501 gcatctgctg gcggtgtttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
     7561 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
     7621 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
     7681 tctacagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
     7741 tcccttcttt ctcgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
     7801 ggggctggga agattgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgctttcc
     7861 aaacggccaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
     7921 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
     7981 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata caggctgttg
     8041 ctggggcggt tcattaacct gcctgcgctg ggaagataaa cactattttg tccagccgtc
     8101 gaaccggcat aaagatatgg gtgagggaga ggcggtcgcg tcttaaatcc cgtcgagcat
     8161 aaaatctaat gctcctttaa tcaacgttcg gtgcgcgctg gcatcgcaaa atttcgttcc
     8221 cagcgcgtta actggaacag ttgcagtctc atgcgtcatg agcacatatt ctttgccgtc
     8281 taccagtaaa aggattgggt taagccgttc tggtgggcga ataaggataa atcgttcaat
     8341 aggtgttaat ggaataacaa tacggcgatt aatcacacca ataatgtcgc tggtaacatc
     8401 gataacaaag ggaaacgcgg cgttcctgct gcgactgcgg tataccgtaa attgcatcgc
     8461 tagaagctcc ggtactcatc agagaaacat ccggtttcat cagccaattg atttaacgca
     8521 gcgatagctt ctgcgttctc ttcacgccaa cgtgttgctg catgtttttt aagttcagca
     8581 ttgagcgcaa ccgtaagggt ggcgctcata tttaagcctg catcacgagc gcgattgact
     8641 aactcacggt cgacggtgac ggtcacactt tgtgtggtac gttttgcagt catgatatcg
     8701 tgctcctgta caatttatac gtctagtata cacggatatc tgcacgtatc agtgatgccg
     8761 gaattctaat atacaaaatt accgccgctc cagaatctca aagcaatagc tatgagagtt
     8821 ctgcgcatca gcgtcgtgga attcgctgaa taccgattcc cagtcatccg gctcgtaatc
     8881 cgggaaatgg gtgtcgcctt ccacttctgc gtcgatatgc gtcagataca gtttttgcgc
     8941 ttttggcagg aactgctcat aaacgcgacc gccaccaatc accatgattt ctggtacgtc
     9001 accacacgcc gcgatggctt catccaccga cttcacccac gttacgcgat cgtccgtacc
     9061 cggttgactg ctgaggataa tatttttgcg tcctggcaac ggacgaccga tggattccca
     9121 ggtatggcgg cccataatca cgggtttatt taatgtgttg cgtttaaacc aggcgagatc
     9181 ggcaggcagg ttccacggca tggcgttttc catgccgata acgcgatcta ccgctaacgc
     9241 cgcaatcaga ctgatcattg agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa
     9301 gcgtaaaccg tcgtcgactg gtgcgaggat gatgttgagg aaaattttat attctgctgg
     9361 cgagtccacg ctctccccct ggactcgccg cattacaatg aaacaaaaac aaacagttag
     9421 ctgtaaagtg tgatttacgt cactctttat taggatgagg gtttcgtttc cggttcatcc
     9481 gccatgttgc cggtatgttt accttcttct gttccctgcc agccatgtcg ttgaattaat
     9541 gacagatgtt cgcggtcctc ggtgatgatc tcacttaaca tcgcgctggt gcgtttatag
     9601 accgccgcgc gggctttggt gtcattctca accattgcca tctcttccac catctgaata
     9661 ttaaagcggc ggaacacatc ggcacgttct cgcgcttcat acggccccag acctaaactt
     9721 tccagcgcca gacgcccggt tttcagcgca ccttcaaagg tttcacgctc cggcttttca
     9781 acgcctgcct gacgcaaacg aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc
     9841 aaatgcggga aatgttcttt caccatctcc gtcagttgca ggttggtttg cggatcgtca
     9901 atagcgttaa tcagcacttc cgctttcgct gctccggcag attccagtaa atccatccgc
     9961 gtggcatcgc cgtaaaacac tttcatacca aatttacgca gcgtttcgat atggtccgga
    10021 tcgtgatcga gcaccaccat tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc
    10081 ccaaaacgac cgaatccggc aatgattacc cgcggctgtt cttcgtcgat ctcatcggct
    10141 tcacgcgcct cttcagtaga agattgctca aggcgattga ggatcaccag cagaatcggc
    10201 gttgctgcca tcgacagcgc caccgccagg gtcagcgatt tcgcccactc cggctccagc
    10261 acattcgcca tctgcgccgc gccaaatacc acaaaggcaa actcactgcc ctgccctaac
    10321 aacaccgcaa accagcgacg ctgtttattt ggcacctgca acggtcgggc aatcagccac
    10381 agcatggcga ttttgatgat gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt
    10441 tcaatcagcg tgccaaagtc tatcgacatg ccaacaccga tgaaaaacag ccccaacagc
    10501 aaacctttaa acggttcgat atcgctttcc agcgcatgac ggtattccga gcttgccagc
    10561 agtacgcccg ccagaaacgc gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa
    10621 ccaaagccaa acacaaggaa taacgccacg gcactaaaca cttcccgcaa gccagagcgg
    10681 gcaacaaaac gcagcgccgg acgcgtaaca tagcgcccca gcaataccac cagtaccagc
    10741 gcacccgcca cttttaacgc cgagagagca aatgcgccca tcgtcgtcga ggcactgctc
    10801 gccgccagta gcggaatcat cgccaccaac gggatcgccg cgatatcctg gaacagcagc
    10861 accgcaaagg cactgcgacc catttgcgtc accatcagat tgcgttcatt catcgcctgc
    10921 atggcaatcg ccgtagagga gagcgccagc gtcatgccga tcaattccgc gacctgccag
    10981 cgcagcccaa gtaacatgca gaacagcccc agcaggccgc cgcaaatcac catctgtaat
    11041 gcgccaccgc cgaacaccgc cgcacgcagc ttccacagcc tttgtggatc gagttcgagg
    11101 ccgataataa acagcatcag caccacacca atttcagcaa agtgcagaat agattcggca
    11161 tcggtcacca gccgcaaccc ccacgggcca ataatgcagc ctgcaatcag gtagccaagt
    11221 actgatccca gaccgaggcg taccgcaatg ggtacaatca gcgccgccga tccgaggtag
    11281 atcagcgcct gaaccagcgt atgactatcc atgatgtgcc tcctgccatt ccagcagacg
    11341 ttgcttatag tggcgcgcct gcccttcgag ggtttcgtcg tcacaaataa aggtacagtg
    11401 catggcaaac ggtggcagcc agttcagccc gcagtagatt gccgtcgcct gtagcggctg
    11461 cgacagcaca tcaaaacccg gatgcgcacc aatttcaaaa tggctttccc cgccgccggt
    11521 cgtcaccgcc cacagcaaat gtttgccatg cagcgccgtg ccgccgtgac cgtaagccca
    11581 gccgtgcgag aagactttat cgatccaaag tttaaggagc ggaggaatgc tgtaccactg
    11641 catcgggtgc tgccagacga tcagatcggc gcgagacagc gcctcctgct cggcggcaat
    11701 atcgatattg aagtcaggat agagttgata aagagagcga atttcgacgc cttccagcgt
    11761 ccttgcctgt tcaagcatcc gtttattcgc atgggaatga tgcggatacg gatgcgcata
    11821 aattatgagg atcatagtta ttagcccgcg tgtttacgcc tttgttttcc cacaaagtgt
    11881 agtcatatat tcacgcggct tatagtcaat aatattgatt cagaaaagag agaaatctga
    11941 acaaattatc ccattctctg cccgcggatc gtcatattgc tggtctgcgc cagtgacatt
    12001 cctcgagtct ccggggcaaa cgctacggaa atcaacaagc caaacagcga gatacccgcc
    12061 cccattagca tcgtgttact gataccgtaa ttattgataa atatcggcag tgcccaggtc
    12121 gaaacaatgg tgccaatacg acttaaggac ataatcacgc ccacggcaga ggcgcggata
    12181 tccgtcggga agagttcatt aggatagagc cactgcaaat tacccggccc gccagagaaa
    12241 aaggcataca ccgcaaaggc catcactacc agccagatcc ccatatccgg gattagcccc
    12301 aaaaccgcca gcgccagcgt catcatggca aagctgccaa tcaacaatgg acgccgtccg
    12361 gcagtgttta gccacagcat cggcggaata cagccgagca taaagaacag gctaatcacc
    12421 acattcccta gtgccgcgtt tttgccaacc cccaatccca acaaaccaac gatttgcggg
    12481 ccaaaggtgt aaatggcgaa cattgggatc acctggcagg tccagatggc ggcaacaaac
    12541 agaacaaaag gaaaatggcg gcgattaaac agatcgcgaa aacgggtttg ctgcggctgc
    12601 tcttcatcga aagccaccgg ttcgccaaac agttttatca tcatctcttc gcactctttt
    12661 actcgccctt tgcgtaataa ccagcgggga gattctggca gttcgaatcg accaatcaaa
    12721 atcaacaaac aagggatcgc cgcgctaccc agcatccagc gccagccgcc ttccacatca
    12781 taaagccagt agccgaccag atcggcgcag gtcgcgccga cataccacat ggcggcgata
    12841 aagctgatgg aaaatgcccg ctggcgggta ctggagaact cggtgatcat tgaggtggcg
    12901 atgggataat ctgcaccgat gacaatgccg ataagtaccc gcatcaccaa cagttcgacg
    12961 ggggatgaaa caaacatcgt cgccacagat atcacaccga tggcgatgat atcaatgagg
    13021 aacattttgc gccgtccgac tttatcggag atatagccaa acagcgatgt gccaacgaac
    13081 agcccggcga gcgttcccgc gcccagcaag ccaatccagt cagcgtccag ttttagcgcc
    13141 ggagtcagtt gctccagcgc cacgccaatc attaccagta cataaccatc cagaaacgga
    13201 ccaccgcttc cccacagcaa aatgcggcgg tgaatagagg agaatttaag atcgtcaaag
    13261 tttctggacg gttgcatgga ttattcctgt tttttgcaat gagagtcagt acggcgttac
    13321 gtatcaaacc aacatcagcc gtaacggaac tccacaccaa aggtgccgcg cgggtattcc
    13381 cactgttcca gcgccgaccc cagccccaga atgcgacagg tgccgcactc cagacatccg
    13441 gcgtaatcga agcgcacact gccgtcatcc tgcttcttgt acagacctgc ggggcacgct
    13501 ttcaccagca gctccagggc ctgtttatcg gcatcggcct tcacaacaat gtgtggatgc
    13561 tcttcatcga cattgaattt attgacgccc agtttgacgt ccacattgac gggagaagtc
    13621 ataaaacggt cactcctttc atgccatcct tgatcagatt gatgaagccc actttcttgc
    13681 cgtggcggag gattttcttg cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt
    13741 cacgcgccac gcccaccgcc agttccgggt agccgctaaa catgcgtggg ttatcaagga
    13801 acgccggtag tttctggtac atgcgcatat cgcgcagcgg gccactctcc agatgctgac
    13861 gatattccgc gagtttttgc ttactgaaat cgtcgctttt catcgctgaa agcacggttt
    13921 ttgctgcagc ttctccggcg gcaatcgcca gatccatacc gcgaatggta aaaccgaggt
    13981 tcatacacat tccggcggca tcaccggcaa tcaatacgcc gtcaccaacc agctccggca
    14041 gcatgttgat gcctgcttcc ggcactacgt gagcggaata ttccaccagc ttgccacccg
    14101 cgatcagcgg tgcaacggcc ggatgttgtt tgaaatcttc cagcatttgc ggcaccgatt
    14161 ttttcgcgtc atgcagatga tgcagaccac aaaccagccc cagcgacagg gtgttttcat
    14221 tggtataaag aaaaccgccg cccatcaggc catcagtagg tgatcccgca aacaggcaag
    14281 ccgcgccctg attaccctgc aactgaaaac ggtcttcgat taccgacttc ggtaactcga
    14341 tcagttcctt cacgccaacc gccacatccg tcggtttgac gcgttttgcc atccccaatt
    14401 tttcggcaag gatggagttc accccatcag caaggatcac cgttttcgct tcaatcacat
    14461 cgccatcggc ttctacacca acgactttgc catcgcgctg tacgaggtta tcgacgcgga
    14521 tcccggtaat taactgcgcg cccgcttctt cggcctgctc catcaaccag gcatcaaatt
    14581 tactgcgcaa aacggagtaa gaacgctggg atggcgaggt ttcgtcaccg ttgcagtagt
    14641 ccatagtcat cgctgacttt tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt
    14701 ctacgggggc ggattcggcg aaacctggaa taatgtgttc cagactgtgg gcatagagac
    14761 gcccgccggt gacgttcttg gcacctgcgg aattgccacg ctcgataact aacacttgcg
    14821 ccccttcgcg ggcgagcacc agtgcggcaa ccgaaccggc aagccctgca ccgacgatga
    14881 tggcgtcaaa gatatcttcg gacataagag ctccctgtca gcggcagagc cgtaatgcag
    14941 ccctgccaga gtggatcaac gcgctaaagc tgcggtcagt gccggaagga tcttcacggc
    15001 atcgccaaca atgccgtaat ccgcgtactg gaagatcggc gcatttttat ctttattgat
    15061 ggcgaaaatg gtttgcgatg cattagcacc aaccatgtgc tggatctgcc cggagatccc
    15121 caccgccaga tacaattcag gtttcagcat caggttggag ataccgacat agcgttcgtg
    15181 ctccatccat ttttcgtttt ccgccaccgg acgagaacag gccaactccg cacctatcgc
    15241 cttgcaaagc tgttctgcca gcgcaatgtt atctttgctg ccaatgccgc gaccgacgct
    15301 gaccaccaga cgggctttgt cgagatcgac gctgttgctc tggcgcgcct gggttgccgt
    15361 gcgggtaatc gccacagccg gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga
    15421 cgcgtctggc tgagccgcat cgaacgtgcc actgctgatg gtcagtaccg catacggcgt
    15481 ggcaatgcgt tcttcgccaa tcgccaggcc gccgtaaacc atgtgtttca ctgttgcttt
    15541 accgtcctgt acgctgacgg tgctggcatc gttagacacc gccgctttaa ggcgataacc
    15601 cagttttgcc gccagtaatt tgccgcgacg ggtgtttggc aacagcacca ggccgtctgc
    15661 gccgtgctgg cgaatagtgt cagccatgac actggcgtaa tcttcgatca tccgatcgtc
    15721 cggtttgccg tttaatttcc agacatgatt agcgccgagc tggattgcct gtgcgccgtc
    15781 ggcatcattg aggacaaagg tgttgatttg attagctaaa gcctgcgcac cgttcatcag
    15841 ttccggcaga cgagaagggg tatcgctgaa tacccagact tgagaaaacg tgttcatagc
    15901 atcccctgta attaaatgac tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg
    15961 ccgtcgcctt caatcacgat gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt
    16021 tctgaccaga cgtccacggc gttaaaaccg atatccgccg ccgaccatac ctggactggc
    16081 tttttcgccg cgccgagaat ggctttcatc gaaggaattt gtggggagtt gatatcagtg
    16141 gaaacagcaa caaccgcagg cagcggaatg cttaaggttt cggtttcatc ttccagttcg
    16201 cgctcaacgg tgagggtatc tgccgtaagg gagataattt tgctgacgcc gttaactgcc
    16261 ggaatattga ggatttcgcc caccagcaga ccaacctgct gggcataaag gtcggaagaa
    16321 ccatcgccac agaggatcag atcaaagcct gctttctggg cggctgcagc cagtgcgctc
    16381 gccgtttgtt gcggcagtgc ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc
    16441 gggccgcgcg atagcacatc tttacgccct ttggcgttgg tcagggcttt accgcccaca
    16501 cttaaggctg tcacctgcgc ctctgctgcc tgttgcttta gctggcaagc cgcttcaata
    16561 gcgttgagat cgtattggct tattttggca tcggctttgc tgaagtctaa tgaaccatca
    16621 gcattattga ccgcaatatc ctgttcatca ggcacgcact tatagcaagt aataatcttc
    16681 attgcatctc cagaaatcat gaaggaaacg tcgttcatat cggtggtgtt aattacagat
    16741 atcacgccct ttaatttgga aataaaactt taatcaccaa tattgaaaat gtcacacgca
    16801 gataaatcca actttcaata
//
LOCUS       DAZUCE010000106        16309 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZUCE010000106 DAZUCE010000000
VERSION     DAZUCE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.106"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..235
                     /locus_tag="ACHY03_002875"
     CDS             <1..235
                     /locus_tag="ACHY03_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906082.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            228..461
                     /locus_tag="ACHY03_002876"
     CDS             228..461
                     /locus_tag="ACHY03_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906083.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMEMQEIDLLHDRYKEQHTGE"
     gene            467..766
                     /locus_tag="ACHY03_002877"
     CDS             467..766
                     /locus_tag="ACHY03_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS3906084.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            763..2163
                     /locus_tag="ACHY03_002878"
     CDS             763..2163
                     /locus_tag="ACHY03_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFS3906085.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            2364..2615
                     /locus_tag="ACHY03_002879"
     CDS             2364..2615
                     /locus_tag="ACHY03_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906086.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            2612..3022
                     /locus_tag="ACHY03_002880"
     CDS             2612..3022
                     /locus_tag="ACHY03_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS3906087.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            3033..3305
                     /locus_tag="ACHY03_002881"
     CDS             3033..3305
                     /locus_tag="ACHY03_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFS3906088.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            3262..3390
                     /locus_tag="ACHY03_002882"
     CDS             3262..3390
                     /locus_tag="ACHY03_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFS3906089.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            3432..3656
                     /locus_tag="ACHY03_002883"
     CDS             3432..3656
                     /locus_tag="ACHY03_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906090.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            3908..4114
                     /locus_tag="ACHY03_002884"
     CDS             3908..4114
                     /locus_tag="ACHY03_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906091.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            4114..5169
                     /locus_tag="ACHY03_002885"
     CDS             4114..5169
                     /locus_tag="ACHY03_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS3906092.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPENCIIEQAKGKKWSEQDKDMHDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            5182..5517
                     /locus_tag="ACHY03_002886"
     CDS             5182..5517
                     /locus_tag="ACHY03_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS3906093.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            5530..5943
                     /locus_tag="ACHY03_002887"
     CDS             5530..5943
                     /locus_tag="ACHY03_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFS3906094.1"
                     /translation="MATNESIMTLPLASKFKVEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVGTAKSILSMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKII"
     gene            6149..6691
                     /locus_tag="ACHY03_002888"
     CDS             6149..6691
                     /locus_tag="ACHY03_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS3906095.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            6948..7229
                     /locus_tag="ACHY03_002889"
     CDS             6948..7229
                     /locus_tag="ACHY03_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906096.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(7831..9291)
                     /gene="phoQ"
                     /locus_tag="ACHY03_002890"
     CDS             complement(7831..9291)
                     /gene="phoQ"
                     /locus_tag="ACHY03_002890"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFS3906097.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(9291..9962)
                     /gene="phoP"
                     /locus_tag="ACHY03_002891"
     CDS             complement(9291..9962)
                     /gene="phoP"
                     /locus_tag="ACHY03_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFS3906098.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(10131..11501)
                     /gene="purB"
                     /locus_tag="ACHY03_002892"
     CDS             complement(10131..11501)
                     /gene="purB"
                     /locus_tag="ACHY03_002892"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFS3906099.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(11505..12146)
                     /gene="hflD"
                     /locus_tag="ACHY03_002893"
     CDS             complement(11505..12146)
                     /gene="hflD"
                     /locus_tag="ACHY03_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFS3906100.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(12182..13288)
                     /gene="mnmA"
                     /locus_tag="ACHY03_002894"
                     /gene_synonym="trmU"
     CDS             complement(12182..13288)
                     /gene="mnmA"
                     /locus_tag="ACHY03_002894"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFS3906101.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(13342..13803)
                     /gene="nudJ"
                     /locus_tag="ACHY03_002895"
     CDS             complement(13342..13803)
                     /gene="nudJ"
                     /locus_tag="ACHY03_002895"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFS3906102.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(13813..14466)
                     /gene="rluE"
                     /locus_tag="ACHY03_002896"
     CDS             complement(13813..14466)
                     /gene="rluE"
                     /locus_tag="ACHY03_002896"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFS3906103.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            14638..15888
                     /gene="icd"
                     /locus_tag="ACHY03_002897"
     CDS             14638..15888
                     /gene="icd"
                     /locus_tag="ACHY03_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFS3906104.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            complement(15991..>16309)
                     /locus_tag="ACHY03_002898"
     CDS             complement(15991..>16309)
                     /locus_tag="ACHY03_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFS3906105.1"
                     /translation="WIVIDTIIQPSCGIFFSAIWGNMKMIIWYQSTIFLPPGSIFTPV
                     KSGIILKDKEYPITIYHIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKICPY
                     GLK"
BASE COUNT         4334 a         3894 c         4251 g         3830 t
ORIGIN      
        1 tatgcgtgat gatcgtttta attccctgaa acaggaattt tccggcgttc ctgatgatgc
       61 ggctgatgcg ctttcgtcaa tgccagaact tattagagcg gcttttttct tactttccac
      121 gagagaatat aaatcaacgg ggcttgatgt actgaatatc gccgccgatt atgcggaata
      181 tgtggcagag gcgcgttata gaagaaaatt tcctgaggat gtaagccatg cgtgatattt
      241 accaccaaac aatagaccgc gcatttcttg cactttctca cagtgaaaac atgctggaaa
      301 tattgcgcat atggcttgaa acacttggcg acaatgaacg cgacaaacaa aaatcaagaa
      361 ttgccacggc attaataacg cttcttgagc ctgtaataat ggaaatgcaa gaaatagatc
      421 tattgcacga cagatataaa gaacagcaca ccggagaata aaaataatga aacttaaata
      481 ttctggctta actgccagtg gcaacactca ccctaaattt acgcgcggtg atatttaccg
      541 cgaccagtac ggcggcacgg taatgattaa gggcgtggaa gaacggcgcg taacctaccg
      601 ccgtgaaggt tacgaatatg attgcgtgat gcctgtttat cagttccggc gtgatttttc
      661 tctggtacag accgcgccgc ataacgtgcc caccagcaac gccagggcac gggcaaacat
      721 ccagaagctg aaaaccatga ttaacggatt caggggcaag aaatgaaaag cgcaccgaac
      781 ttaaaaaaac agccttacga caagatgacc gaagtcatta tttttgcggg tagtgatgcc
      841 tgggcacatg cgaaacagtg gcaggaacag gacgggcgac tggctggcga taatgtgcct
      901 cccgttgtgc tggctgatga tcaactggat gaactggcag acctgagaat catcgacgag
      961 gggcgctatt gtgtccggct gtacaaggca ggccacatca ggccatcaaa tattaatgcc
     1021 attgcgcaca agctggcggc ggcgggtgta actgatgcga attattaccc cgaagggatg
     1081 cacagccata tgcgggagaa ctggcgcgaa tacctggaac gggtgcgcgg gaaagagccg
     1141 gcggaagaaa aaaaccacca gcgaaaaacc acgctaccga tgagcgttgg atctaccgga
     1201 tacgacacgc aactggatta cgtggttaag gggattattc cggcggtatc gctatgcagc
     1261 atatacgggg ctagcgggtc ctataaatca ttccttgccg gatcgtgggc gtgccatgtt
     1321 gccactggtc gccagtgggg aggccgcagg gttgcacatg gtgcggttct ctatgtggtt
     1381 ggtgaaggcg gtataggtgt tccgcgtcgt gtaaaagcct gggaggttgt gcacgatgag
     1441 caggtgaaaa atctgtatct ggtaaaccgc cccatctttc cggctgcccc gcttgatgtt
     1501 gatgaaatgg ttatcgctgc ccgtcaggtg gagcgggaaa cgggtaaacc tgtacgcatg
     1561 attattctgg atacgctggc gcgttgcttt ggtgggaatg atgaaaatga ttcccgtgat
     1621 atgggggcgt ttatccgtgg ttgtgacgaa ctgaaacgac gcacaggggc cacggtgctg
     1681 gtggttcacc attccggcaa ggatgagacg aaaggcgcgc gcggttccag tgcatttcgt
     1741 gcttcgctgg atgctgaata ccggatacgc agggaggacg caggaagcga agcgctggtt
     1801 atctcatgca ccaaaatgaa ggacgcggag gaactcaaag aagccgcata tgacttacgc
     1861 gtggtggagc tttttaccga cgctgacggt gaattaatca cgtcgctggt ggtggtggat
     1921 gatccgcgcc ctcctgttga actggagcgc atcgaggagg cagggaacaa gacggaaaac
     1981 cataccgcgc tatgggggtg catccgttca cgcacacaga acggcgacaa gtgcacgatc
     2041 ccgctgttac gtgatgacat gaaaaagctg gggtatgaaa tgaaaaactt ccggcgctgg
     2101 ctgtacaagc tggaaaaaga tggggttatt cgtatcgatg gggatgatgt agcgccgcta
     2161 taaaagtgag gagcaaaagc gagggggata gaaagagggc caaaattagc ccgctctccc
     2221 tcacttttcg acctgtatac atcctcaaaa gtgaggggta aaaaaatact tatgaaacac
     2281 acacatagaa aaaccgaaaa tcccaactgc gacgaagtga gacgcttgaa aaagtgaggc
     2341 gaaaaagtga gaggttgcga gaaatgaccc aaaaacgcag agacagaaca gagccaaaat
     2401 ataaagcgtt agacatgact gagcacacct taaaggtggc aatcagaacg atagaccgcc
     2461 acacgcggga aggatacgcg aaggaacatc ccgacctgat aagcgcattc atgaccacgg
     2521 cggcggcaaa ctttgccacg ctgacagaac gggagattgc cgaagcggaa caggtaacaa
     2581 ccatcaacgt taaaaccgga gaggtggaat tatgacggca cagatagccg cttacgggcg
     2641 gctggtggac gacccgcagg taaaacagac cagcaagggc acaccgatga cgctggcgcg
     2701 tatggcagta tctttgccat gcagccaggc acaggacgga caggcgacgt tatggttatc
     2761 ggtcatcgca tttggcaaac aggccgactt cctggctaaa catcaaaaag gcgatgttgc
     2821 cagcgtatcc ggcacgatgc aggtaagcca gtggaccgga cagaacggag aaacgcggca
     2881 gggctggcag gttatcgcag acagcgtaat cagtgcccgc gcggcacgtc caggcgggaa
     2941 cagacgcaaa accacaggca cacagggtaa tcagccacca gcgggaggcg atgaccctta
     3001 cggtgatgat atttcgttct gagggggtga cgatggtaca tgaccgcata gcggaggaac
     3061 tggaggcgaa aggcttttac cggagggcgg cggcgcgatg gggtgaagtc atgcagctgg
     3121 tggagacaga caaggaacgg catcacatca cgatgcgacg gctggaatgt tcaaggaagg
     3181 cacagagggc accggagccg ccggataatt tcggagacct gaaaaaggca gtcgatcgca
     3241 cttatgccga aatgggtata gatggtgctg gtgatgaaat atggcgcaat taccaggaca
     3301 gctaatcaaa cagccggaga aatccggctt tttttgcacc agttgaaacg gtatggcgca
     3361 ttaccgggtt ttcgtcacgg tcaggcatag ttactatctg aaacaaacag acacaacaga
     3421 ggaaaaaaac aatgccgatg aaatttgatg agatattaaa acagcgtgat aaataccatg
     3481 ctgacaacat ggagacgatg agcatcaatg attaccgcgc attcctggag acgggcgcac
     3541 tgattgaaaa ggatcagcat ggttttgtga gatgtgctct atccggtgaa atgctggcgg
     3601 taaatcctga acagatagat gcattgatag aatttctgaa agagatcaga gactgagcca
     3661 gcacacagca cacatagccg gagcaatccg gcttttttgc gcccaaaaaa agcccgataa
     3721 ggtcagaggg ttcttatcgg gcttttgcat atgaggtttt tggatgcact ggcgttcgtg
     3781 atcgggataa tcatttcata atttgcaaca taactcaata tcattgcata aaatgcaatt
     3841 ctgattataa tcaggactgg ataaacatcc agttatgatt tttttagtcg aagaggaatt
     3901 tcttactatg gctgaagaga aaaaaggcgg tgtttcggtg tacataagcc ccgaaatcgt
     3961 ggaggtgctc aagcagcgcc acaaaaaaaa ctatgaagct ggcgttgcgg ctggactgga
     4021 tccgctgatg acgccggagc cgtcgatagg ttcacttgta cgctcttatt tacttgcggc
     4081 gcttgggatg cataaaaatt atgggggtga ataatggcag gcaaagcaac ggcacttaac
     4141 actaaccagc ttttcatgta cctgaatcgc ggggatattg cggattttaa attcagccct
     4201 ctgtttacca cgctgttttt cccgaacgtg gcgacattca gcacgcaaaa catcatgctg
     4261 gataccctgg acattgaaga agtcaccatg tcggcgtttt gttcgcctat ggtgggtagc
     4321 caggttcagc gcgataaagg gtacgaaacc agcacaatca aacctggcta catgaagcca
     4381 aagcacgaaa tcgatccaac gaaaaccatc atgcgcatgg ctggagaaga tccggcacag
     4441 cttaacgacc ctacctatcg ccgtatgcgc ctgattactg gcaacatgcg ccgccagata
     4501 aacgccatta aggcacgcgt ggaatggctg gcggtgaatg cggtaacgac cggaaaaaac
     4561 atcattgagg gcgaaggcat agaacgctat gaaatcgact ggaaaatacc ggaaaactgc
     4621 atcatagagc aggccaaggg taaaaaatgg tccgagcagg ataaagacat gcacgaccca
     4681 atctatgaca tcgagcttta tgctgatcag gctggttgcc ccgcaaacgt catgattatg
     4741 ggcgctgagg tatggcgcac attacgcagc tttaaaaaat tccgtgagct gtacgatctt
     4801 tcccgtggtt cagaatccgc cgccgaactg gcctgtaaaa acctgggcga agtggtgagc
     4861 tttaaaggct atcttggtga tctggccctt atcgtctatt ccggcaaata cactgacagc
     4921 gatggtaccg aaaaatattt ccttgagcct gatttgctgg tcctgggtaa caccaacaat
     4981 aaagggctgg tggcctatgg tgcgataatg gatcaggaag cggtaagaac gggcgcaaca
     5041 caaaacatgt tttacccgaa aaactggatt gaggacggcg atccggcgat tgagtacgtg
     5101 cagacacaca gtgcaccgca gccggtaccg gcagatattc gcaaatttgt taccgtcaaa
     5161 attggttaac ggggggattc tatggacact ccatacattg agttatttgc aggcagtcag
     5221 caggtatcca cgacgttggt acattttgcc gctgatgctg gcgttattca ggaatttacc
     5281 ccgctgatgc tggcagacaa tggcgagttt aagccgtggg atggtcagga atctggcaag
     5341 gctgtttatc tgacctcgta ccctgtggac acgtcaaagc agaaatcagc acagtgttac
     5401 aagacgggga tattcaatat tgccgccgtt aactggcctg agagcgtcga caccgatgcg
     5461 aaaaaatgcg ccgcctttgc gggttccggc gtatccgttc agccgctggc ccgataagca
     5521 gggggaacga tggcaacgaa tgaaagcatc atgacgctac cgctggcgag taaatttaaa
     5581 gtcgaagcgc gggcaatggc tgacagaggt ttatcaacct atgaggccgt atatcaacta
     5641 aacaaactgg aagagcagga caagccgcgc gctgatgcga ttatggcgct tcatgagcat
     5701 aaagactatc agccgctgtt gcgtgcaatg gcaaacgtgc cttgtatcga tgttggtacg
     5761 gctaaaagca tccttagcat gaccatagag caggaacgtc cgaaggttgc gccggaactt
     5821 accgcagcct ttgaaaactt tatggacatg cacagcccgc aagccgtatc agctggcatg
     5881 gcatacgatg gcagaaacca gggcgatgac ggcgacatcg atcgcatact gaaaatcatc
     5941 tgagacaagg ccggagaaat ccggcttttt tacgggtcct tcctggaatt atggcccgtt
     6001 acggggcggc gacctcgcgg attttcgcta tttatgagcc ttttcagggt ggtggtggtg
     6061 gttttgttgt ttgatctatc tttatgaatg aaaagggaaa gatgcaagca atacaccaac
     6121 ctgaagcagt aattaagttg gtgtattaat gaaatctcat ctgatgaaca aaaaaaacat
     6181 ggcgaaaagc tgccgtgtaa gtgcgacagc gttcgacaag tggggagtaa ctcccgttga
     6241 gcgtaaaggc cgtgaggcgt tttatgatgt tgccagcgtg atagaaaata gggttaacaa
     6301 tgcaattagc cagcttataa acgacaaagg cgagattgac gatgatgaac ttttacgagt
     6361 caggatcaga ttactgacag cacaggcaga ggcgcaggaa cttaaaaacg atcgcgatcg
     6421 cggtgacgtg attgatactg agttttgcct gtacgcgctt tcaaagctgg cgagtcagat
     6481 ttcatcaatc atggacagcc tcccgcttac tatgcaaagg agcttcccac agattacccc
     6541 cgccatgctg gacaggctta aaagggaagt ggttaaagcc tgtaatgcca gtgccagagt
     6601 tgccgacaac ctcccacaga tactggctga ttacttgaaa gaaacaaccg gaaacgtacc
     6661 ggaaaagttg ctacagaaga aaggcgagta acagacgcgc aattattgaa caaaaatgag
     6721 aaaggatctg ataatggcat tttctggcta aaatgcctcg tgtcagatcc tttctttttt
     6781 ttaatatttt cattgttaaa cagtctgtta cagagaagaa gatcggatct gacttttgcc
     6841 ggaaaattct cataaacaga taaaaaccgc gaggtcgccg ccccgtaacg ggccataatt
     6901 ccaggaagga cccgacgaca ccagactatc agagcgatgg gggcacaatg acagaagccg
     6961 aactactggg attaatccgc cgcgtcgccg gaatcagcca gcagcatgac gaacaggcca
     7021 cacagccgga cagcgtgacc gctgaaaatt atgtgcgtgt tgtagcggaa gtgatgcgcc
     7081 gtgatggtat agaacttaat ggcgttgata tgcgcaacat acggaaccgc gttcttgaaa
     7141 tgctggccta caatcgccgc gttgaactgt atcgggagaa agaaaaaata acgtaccact
     7201 ggaagaagcc ggaacggctg cggcggtaac ttgctgatat tcccgataac gcaaaattgc
     7261 gctggctaca tccctcacat ctgaggactg ttaatcagaa ggccggaata tattcacaaa
     7321 ctacgaaatt ttcgtagttt aattaatggt cagattaatc gctaacccgc tgatattttc
     7381 ggaaacctca atttgaggaa gtcggaagcg tcacccttca tattctgaat ggtcataatg
     7441 accacgcaga ccatcaaggc aaccacaacg aacatcgtta cggttggagc ttttacccca
     7501 ctgggggaaa ggtattacga taatcataac acctaccgaa caggtaaaac ccaccagcct
     7561 gattaacagt taaccggaaa aaaaatccgg tagccccgtt atggggatcc ccatatcgac
     7621 attaacgccc ctcatgaatt ggtgcgcttc cccctggaaa gattaccagc ctgtatattt
     7681 cttgtgtcta tttgttccac gttgtttcat acagtgcacc gaacggtgta gttaatggtg
     7741 tagtcaattt gctgttttta gcactttttg cagtgtggat tgtttaatta aatcaatggg
     7801 ttgccttgct gtggtgataa aaggacatat ttattcatct ttcggcgcag aatgctggcg
     7861 accaaaaatc acctccatcc gcgcaccgcc aagcatgctc tctccggcga cgattttacc
     7921 ctcatattgc tcggtgattt cgcgggctac cgccagcccc acaccttgcc cagggcgtaa
     7981 agtatcaacc cgttgaccac ggtcgaaaat gacctctcgc ttgcttaatg gaataccggg
     8041 tccatcatcc tcgaccacaa tatagagatg ctcgtcggtt tgccttgcag aaatttcgac
     8101 aaactcgagg caatatttac aggcattatc cagcacattg cccatcacct cgacaaaatc
     8161 gttctgctca ccgacaaagc tgatctctgg cgaaatatcg agagagatat tgaccccttt
     8221 gcgttgatac actttgttca gcgccgaggt gagattgtcc agcagtgggg cgaccggatg
     8281 cagctcgcgg ctaagcaatg tcccgccgcg catactggca cgatgcaggt agtagccaat
     8341 ttgctgtgaa atgcggctga tttgctccag cattaccggt tcagcatcac tgacgctcat
     8401 cttttcacta cgcagagaac gcagcgtact ttgcagcacc gccagtggcg ttttcagact
     8461 atgggtcagg tcggtgagcg tcgtacgata tttgtcgtaa cgttcgcgtt cactttttaa
     8521 caatcggttc aggtttcgta ccagactggt cagttctcgc gttgtggctg gattgagcaa
     8581 ttcgcggtta tgttcttcca gttcgcggac ttcttttgcc agggcttcga tggggcgtaa
     8641 actccaccag gcggcgaccc acagcagcgg gatcactaac agcagattgg ctgagagcac
     8701 atagataaac cagctccaga ccatatagga actttttagc tccaccggaa tggtatccac
     8761 caccacaatg gttaactttg gcatccgcga tgttgccggg tagacgttta ccgccaccga
     8821 gtgggtcatc tccgcgtcgt catcatcttc ccgcacttcc tgcaactgtt gctgtatcga
     8881 atgatctcca ctcagcaaga ggctggtatc gttaacatcc gcttcaattt catgaaaacc
     8941 attcgatttc agccagtcag gctggatcat cttcatcagc cagggcacgt cacgttgcgc
     9001 ccataaaagc tgcccgttct catcataaat tagcgtcatg gtggggcttt gcttgtcgat
     9061 attttcgggt aactcgacat gcaacttatt gttttcccac ttcgcaaggg tatagaacag
     9121 attgctctcg ccacgtaaca gccgaaacgt agttttatcg aaactgacgc tataaccgat
     9181 cagcgcgacc attccgtagg caagcgaaag caccagtact accgctgccg ttgctaacag
     9241 aaaacgtacc cgcagcgaga gcgggaaaaa aagacgcagt aattttttca tcagcgcaat
     9301 tcgaacaaat agccctggcc gcgaacggta gtaatcactt cttgcggata ttgtgcctga
     9361 atttttttgc gcagacgtcc catcagtaca tcaatggtat ggctttcccg cagctccgca
     9421 tccggataaa gttgtagcat taacgaatct ttgctgacca ctttgccatt attgcgtatc
     9481 aacgtttcca taatggtgta ttcgaacgcg gtcagtttga tcacttcgtc attaatagat
     9541 aattcacggc gagagagatc aacctgaaac ggggggagcg aaatgacctg tgaagccagg
     9601 ccgctattac gccgcattaa tgcctgcatt cgcgccatca cctcttcaat atgaaacggt
     9661 ttagtcacgt aatcatcagc accggcactt aatacttcga ctttgtcctg ccagctttca
     9721 cgagcggtta ataccagaat cggcagtgaa acatcattgc tacgccagcg gcgaatcagt
     9781 gacagaccgt cctcgtctgg caatccgaga tcgacaatcg caatatccgg taaatgttca
     9841 ttgagataat aatcggcttc tttggcatct tctgcatcat cgacctgatg accagcatcc
     9901 tgaatctgaa ctttaaggtg gtgacgtaac aacgcattgt cttcaacaac cagtacgcgc
     9961 atttttattt ctccctgtct taattattaa aatagtgtaa cgcgattatg tggttatggg
    10021 ggtaaacatt aaataaacca gcgggggagg ggaggtaaag tgaaaaaata aaaagcggat
    10081 aatcttaata agcaggccgg acagcatcgc catccggcac tgatacgagt ttatttcagc
    10141 tcatcaacca tggtgatggc gcgaccaatg tagtttgccg gcgtcatcgc tttcaggcgg
    10201 gctttctctt cttctggcaa cgccagaccg tcgataaact gcttcatgcc ttcggcgtca
    10261 acgcgcttac cgcgagtcag ctctttcaat ttctcgtacg gtttttcgat gccatagcga
    10321 cgcataactg tctggattgg ttctgccagc acttcccagt tgtgatccag ctcatccagc
    10381 agatggtcac ggttcacttc cagtttgctc acgcctttca gggtggattg atacgcaatc
    10441 agcgcataac cgatacccac gccgaggtta cgcagcacgg tggagtcggt caggtcacgc
    10501 tgccagcggg aaaccggcag tttgcttgcc agatgctgta ataccgcgtt ggaaaggccc
    10561 agattccctt cggagttctc gaagtcgatc gggttaactt tatgcggcat ggtggaagaa
    10621 ccaatctcac cggcgatggt tttctgtttg aagtggttaa gggcgatata accccagacg
    10681 tcacggtcaa agtcgatcag aatggtgttg aagcgcgcaa cgcaatcaaa cagttcggca
    10741 atgtagtcgt gcggttcaat ctgggtagtg tacgggttcc attgaatacc cagcgaggtg
    10801 acgaactctt cgctgaactg atgccagtca acttccgggt aagcggcgat gtgggcgtta
    10861 tagttaccga ccgcgccgtt gattttgccg aggatctcca cctggttaag ctggcggtac
    10921 tggcgctcca tacggtaggc gacgtttgcc atctctttac cgatggttga cggcgtggct
    10981 ggctgaccgt gggtacgaga cagcagcggg atatcgcggt actgaacggc gagatcttta
    11041 atgccatcaa ttagttgacg ccagtacggc aggatcactt catcacgcgc ggttttcagc
    11101 attaatgcgt gggagaggtt attgatatct tccgaagtac aggcaaagtg gatgaattca
    11161 gaaaccgcgt gcagttccgg gatctccgcc actttttctt tcaggaaata ctcaaccgct
    11221 ttaacgtcgt ggttagtagt acgctcgatg gttttgatgc gtgctgcatc ttcttcgctg
    11281 aaactggcga caattgcatc aaggtaaccg attgcgtcgg cagcaaaagc aggaacttcc
    11341 ttgatcgctg cgtgcgcggc cagtttttgc agccaacgta cttcaacttg tacacggaat
    11401 ttcagcaaac catattcgct gaaaatcccg cgcagcgcgc tgactttatc gccgtagcgt
    11461 ccatcgacag gggaaacggc ggtcagtgag gataattcca tagatcacaa ctccggggtt
    11521 aaatgagcaa gaatttgttt tgcctgagtg gtcaggcgat tacgagaaaa catcagttgc
    11581 agacgtccgc cgccgacctg gtgccagagc acggcggcgc gaatgcctgc cagcagggtt
    11641 gcgcgaactt tcgcctgcac ttgtgggctt tgcagtacag caggggaacc ggtgacctga
    11701 atgcgcgggc caagcgggct gatcacatca acatagatag cagccatcgc gctcatcagc
    11761 gtttcggact gtaaatcgaa gtgttcgagc tggcgttgca ggccgttgat ccggttgccc
    11821 agagtgtcga gcgcgccttt cgctgaggag agtttgcgct caagcaccat caagctgagt
    11881 gtgtagcggg ttaactcagc gtttaagccc tggcgactgc tggcattgag cacgccgagc
    11941 agggtttcca gcccgacgcg caggttggct tcgctaccgc caaaaaccgc cagcgtcgag
    12001 ctggggttca tatcaataat actgttgagt gagacgtgta gcgcatcggc atcacaatgc
    12061 ccctggtgag cgagttgttg caccaggcgt gccgactgac aaataccggc cagggcgagg
    12121 gtgatgtcat agtaattctt tgccacgttc actgcttcct tgttgtaatt aaagacaata
    12181 atcagaccgg cagcggcaga cgctgctcaa taataccgcc accgaggcac acttcaccgt
    12241 tatagaagac ggcagactgg cccggcgtca cggcggcaac cggttcatcg aaaatcactt
    12301 caatgcgatc atcgtccagc gccttgacgg tgcaagggat atcggtctgg cgatagcggg
    12361 tttttaccgt gcaacgcata gtgccggtga atggttcgcg atcgacccag tgcaactgct
    12421 gggcaatcaa cccgacagac atcagccgcg ggtgttcatg gccctgagcg acaaccagaa
    12481 tgttgttttc gacgtctttg tccaccacat accacggttc ttcggtacct tctttggtgc
    12541 caccgatacc cagaccttta cgctgaccga gagtgtgata catcagcccc tggtgctcac
    12601 caatttcatc gccatcgacg gtaatgattt tgcccggttg cgccgggaga taacggccca
    12661 ggaactcgcg gaatttacgt tcgccaatga agcaaatgcc ggtagagtct tttttcttcg
    12721 cggtgaccag accaagatct tcagcaatct tacgcacctg cggtttttcc agttcgccga
    12781 ccgggaacag gctttgcgca atctgctcat ggctgagcgt ataaaggaag tagctctggt
    12841 ctttattgct gtccagacca cgcagcaggc ggctcttgcc atcgacatcg gcccgacgta
    12901 cgtaatgacc ggtagcgata taatcggcac ctaaatcttc ggcggcaaat tcgaggaagg
    12961 ctttaaattt gatctctttg ttgcacagaa tatccggatt cggcgtgcga ccggctttat
    13021 attcggcaag gaacagttcg aagacgttgt cccagtactc ggcagcaaag ttaacggtgt
    13081 gcagttcaat gccgagcttg tcgcagacag cctgggcatc agccagatcc gctgccgctg
    13141 tgcaatattc ctcaccgtcg tcttcttccc agttcttcat aaacaggcct tcaacctgat
    13201 atccctgttg ttgcaacagc caggcagaaa cggaggaatc gacaccgccg gacatgccga
    13261 cgattacttt ttttgcggtt tcagacattg gatcactcac gacattgaac ttcaaggcgg
    13321 cgtattctat cacgcagcct tttagatgac accctttgta aaaggccagt taaaatcgcc
    13381 aatcatctcc agcggatagc gttgcccgct ttgataacaa cggatacttt ccgccaccag
    13441 cggcgaacga agatttgacg cctttaaaat ttcttcggcg ctgacccaac gacagcagtc
    13501 gatatcgctg tcatgaggct gggtcgggca tatttgctca agctcaatgg caaagaggaa
    13561 acgcaaaaac ggcgttttat ctggcgcaat ccactgatgc atacgaataa agtgttgcgg
    13621 ctgcgcgctg atgccggttt cttcccacag ctcacgggcg gcggcttcca ctaaggtttc
    13681 atcggcttcc agatgccccg ccggttggtt ccataacgct ttaccattaa tcgtctcttc
    13741 aacgactaaa aatttgcctt ctgcgtgcac cacgcaagca acggtaacgt gcggtttaaa
    13801 cattttgcat ccttaatctg tcacttctcg ccattcgcca ttagcaagat tatccaaaga
    13861 gtaatcaccc atcgcatagc gaatcagtcg cagcgtgggg aaaccaacat gggcggtcat
    13921 gcggcgcacc tggcgattac gtccttcata taaggtgatc ttcagccagc tggtgggaat
    13981 acttttgcgt tcacgaattg gtggattccg cggccataac cacgcaggtt cgtcaaccag
    14041 ctccgcgccg gcgggcaggg tagggccatc atttaaggtt acgccattgc gcaaggcttc
    14101 aagtgcgtct tgtgtgggaa taccttccac ctgcacataa tagattttcc cggtgcgttt
    14161 gcccggctgg gttaaacgcg cctgcaacgc gccgttattg gtcagcacca gtaacccttc
    14221 gctatcgcgg tcaagacgac ctgctgcata aacgccctga accgggatga attcttttaa
    14281 tgttttgcgt ccggcttcat cggtgaactg cggaagaaca tcgtagggtt tattgaacag
    14341 gatcacacgc gtgggctggt tttcaggttt acgcctggta gaacgttgcg agctgaatcg
    14401 cttaacctgg tgatttctaa aagaagtttt ttgcatggta ttttcagaga ttatgaattg
    14461 ccgcattata gcctaataac gcgcatcttt catgacggca aacaataggg tagtattgac
    14521 aagccaatta caaatcatta acaaaaaatt gctctaaagc atccgtatcg caggacgcaa
    14581 acgcatatgc aacgtggtgg cagacgagca aaccagtagc gctcgaagga gaggtgaatg
    14641 gaaagtaaag tagttgttcc ggcacaaggc aagaagatca ccctgcaaaa cggcaaactc
    14701 aacgttcctg aaaatccgat tatcccttac attgaaggtg atggaatcgg tgtagatgta
    14761 accccagcca tgctgaaagt ggtcgacgct gcagtcgaga aagcctataa aggcgagcgt
    14821 aaaatctcct ggatggaaat ttacaccggt gaaaaatcca cacaggttta tggtcaggac
    14881 gtctggctgc ctgctgaaac tcttgatctg attcgtgaat atcgcgttgc cattaaaggt
    14941 ccgctgacca ctcctgttgg tggcggtatt cgctctctga acgttgccct gcgccaggaa
    15001 ctggatctct acatctgcct gcgtccggta cgttactatc agggcactcc aagcccggtt
    15061 aaacaccctg aactgaccga tatggttatc ttccgtgaaa actcggaaga catttatgcg
    15121 ggtatcgaat ggaaagcaga ctctgccgac gccgagaaag tgattaaatt cctgcgtgaa
    15181 gagatggggg tgaagaaaat tcgcttcccg gaacattgtg gtatcggtat taagccgtgt
    15241 tcggaagaag gcaccaaacg tctggttcgt gcagcgatcg aatacgcaat tgctaacgat
    15301 cgtgactctg tgaccctggt gcacaaaggc aacatcatga agttcaccga aggcgcgttt
    15361 aaagactggg gctaccagct ggcgcgtgaa gaatttggcg gtgaactgat cgacggcggc
    15421 ccgtggctga aagttaaaaa cccgaatacc ggcaaagaga tcgtcattaa agacgtgatt
    15481 gctgatgcat tcctgcaaca gatcctgctg cgtccggctg aatatgatgt tatcgcctgt
    15541 atgaacctga acggtgacta catttctgac gccctggcag cgcaggttgg cggtatcggt
    15601 atcgcccctg gcgcaaacat cggtgacgaa tgcgccctgt ttgaagctac ccacggtact
    15661 gcgccgaaat atgccggtca ggacaaagtt aacccaggct ctattattct ctctgctgag
    15721 atgatgctgc gccacatggg ttggactgaa gccgcagacc tgattgttaa aggtatggaa
    15781 ggcgcgatca acgcgaagac tgtaacctat gacttcgaac gtctgatgga aggcgctaaa
    15841 ctgctgaaat gttcagagtt tggtgaagcg atcatcgaaa acatgtaatc tctccatgag
    15901 ttaaatattg aaacgggcgt ataacacgcc cgttgtttta tttatgtgga tattattaat
    15961 agcatatcga gcatatttat atgaagccca ttacttgagc ccatatgggc atatttttat
    16021 aatgcaacta ttatgtaaac atttatttgt tattttgctt tctcctggag gacactcttg
    16081 actgcttttg agtaaactcc ataaatcctt gttgaatggt gcgatgtgat aaatagtaat
    16141 aggatattct ttatccttaa ggataatacc agacttaacc ggtgtaaata tactgccagg
    16201 agggagaaat atagtagatt gataccagat gatcattttc atattacccc atatggctga
    16261 aaaaaatatg ccgcatgaag gttgaattat cgtgtcaatt actatccac
//
LOCUS       DAZUCE010000107        16250 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZUCE010000107 DAZUCE010000000
VERSION     DAZUCE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.107"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(88..1218)
                     /gene="dnaJ"
                     /locus_tag="ACHY03_002899"
     CDS             complement(88..1218)
                     /gene="dnaJ"
                     /locus_tag="ACHY03_002899"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFS3906106.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1307..3223)
                     /gene="dnaK"
                     /locus_tag="ACHY03_002900"
     CDS             complement(1307..3223)
                     /gene="dnaK"
                     /locus_tag="ACHY03_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFS3906107.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3600..4004
                     /gene="yaaI"
                     /locus_tag="ACHY03_002901"
     CDS             3600..4004
                     /gene="yaaI"
                     /locus_tag="ACHY03_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFS3906108.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWLNINS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4030..4743
                     /gene="msyB"
                     /locus_tag="ACHY03_002902"
     CDS             4030..4743
                     /gene="msyB"
                     /locus_tag="ACHY03_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFS3906109.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            4892..5458
                     /gene="satP"
                     /locus_tag="ACHY03_002903"
     CDS             4892..5458
                     /gene="satP"
                     /locus_tag="ACHY03_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFS3906110.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5493..6080)
                     /gene="mog"
                     /locus_tag="ACHY03_002904"
     CDS             complement(5493..6080)
                     /gene="mog"
                     /locus_tag="ACHY03_002904"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFS3906111.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6195..7148)
                     /gene="tal"
                     /locus_tag="ACHY03_002905"
     CDS             complement(6195..7148)
                     /gene="tal"
                     /locus_tag="ACHY03_002905"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFS3906112.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            7427..8857
                     /gene="yaaJ"
                     /locus_tag="ACHY03_002906"
     CDS             7427..8857
                     /gene="yaaJ"
                     /locus_tag="ACHY03_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFS3906113.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAIIRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            8927..9703
                     /gene="yaaA"
                     /locus_tag="ACHY03_002907"
     CDS             8927..9703
                     /gene="yaaA"
                     /locus_tag="ACHY03_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFS3906114.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(9856..10152)
                     /gene="yaaX"
                     /locus_tag="ACHY03_002908"
     CDS             complement(9856..10152)
                     /gene="yaaX"
                     /locus_tag="ACHY03_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFS3906115.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10366..11652)
                     /gene="thrC"
                     /locus_tag="ACHY03_002909"
     CDS             complement(10366..11652)
                     /gene="thrC"
                     /locus_tag="ACHY03_002909"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFS3906116.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11653..12585)
                     /gene="thrB"
                     /locus_tag="ACHY03_002910"
     CDS             complement(11653..12585)
                     /gene="thrB"
                     /locus_tag="ACHY03_002910"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFS3906117.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12587..15049)
                     /gene="thrA"
                     /locus_tag="ACHY03_002911"
     CDS             complement(12587..15049)
                     /gene="thrA"
                     /locus_tag="ACHY03_002911"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFS3906118.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15075..15194)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15130..15195)
                     /gene="thrL"
                     /locus_tag="ACHY03_002912"
     CDS             complement(15130..15195)
                     /gene="thrL"
                     /locus_tag="ACHY03_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFS3906119.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15409..16095)
                     /gene="yjtD"
                     /locus_tag="ACHY03_002913"
     CDS             complement(15409..16095)
                     /gene="yjtD"
                     /locus_tag="ACHY03_002913"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFS3906120.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGILEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3744 a         4334 c         4012 g         4160 t
ORIGIN      
        1 tgatccccgc cactttacag gtgctcgcat atcttcaacg caccctattt ttacccaggc
       61 ctgcccacgg gcaggctttt ggggaggtta gcgggtcagg tcgtcaaaaa acttcttcac
      121 accatcaaag aagctctttg agcgcgggct gttgtgctcg ccggttgggc caccgaagct
      181 ttcttgcagc tcttgcagca gctgtttctg cttctcgttc aggcctacag gtgtttcgac
      241 gacaacgcgg cacagcaaat caccctgtgc gccaccgcgg acagacttga cgcctttacc
      301 gcgcatacgg aatagcttac cggtctgggt ttcgccaggc actttcagtt tgacgcgacc
      361 atcaagggtc ggtacttcga tttcgccacc cagcgccgcc atagcgaagt tgatcgggac
      421 ttcgcaatac aggttgttgc cttcacgctc gaaaatcggg tgctgtttaa cctgaacctg
      481 aacgtacaga tcgcctgccg gtgcgccatg ctcgcccgct tcaccttcgc ccgcaagacg
      541 gatgcggtct ccagtgtcca cccctgccgg gattttaacg gacagcgttt tgctgcgctc
      601 aacacgacca tgaccatgac atttgttgca cggatctttg atcagcgtac cgcggccctg
      661 acagtgtgga caggtctgct gcacggcaaa gaaaccctgg cgcatctgca cctgaccaga
      721 accatgacag gtcggacagg tctgcggctg tgtacctggt tttgcaccgc taccgtggca
      781 aacgtcacac tcttccagag tcggaatgcg gatctctttg gtcacgccac gtacagcttc
      841 ttcgagggtg agctccatgt tatagcgtaa atcagcaccg cgcgccgcac gttgacgacc
      901 acgtccgccg ccaaaaatat cgccgaaaac gtcaccaaaa atatcgctga agtctgcgcc
      961 gccgccaaaa ccgccgccgc ccatgccacc ttgctcaaac gcagcatgac catactgatc
     1021 gtatgccgca cgtttttgcg agtcggtcag aacttcataa gcttccttga tctctttaaa
     1081 tttcgcctcg gcctctttgt caccctggtt acggtccggg tggtatttca tggccaggcg
     1141 tttgtaggcc tttttgattt cacgctcttc cgctgttttg gaaacgccta aaatctcgta
     1201 ataatcttgc ttagccatct tttttaaatt gcccctagat gaatgcacgg gcggagagga
     1261 aattcccctt cgcccgtgtc agtataatta cccgtttata gggcgattat tttttgtctt
     1321 tgacttcttc aaattcagcg tcgacaacat cgtcatcttt cgcgttgttt gcagaagcat
     1381 cagcaccggc agtctgctgc tgggcatgtt gctgctgggc gatttccatc agtttctggg
     1441 aaacctgtgc cagttcctgc attttcgctt cgatagcggc tttgtcttca cctttcagag
     1501 cagtttccag tgcagtcagc gcagactcga tagcagtttt gtcgtcagcc ggcagtttgt
     1561 cgcctgcttc ttcaacctgc ttacgggtgc tgtgcagcag atggtcgccc tggttgcgag
     1621 tctgtaccag ctcttcaaac ttacggtcag cttcggcgtt agcttctgcg tcgcgtacca
     1681 ttttctggat ttcatcttcg ttcagaccag aagacgcctt gatggtgatc ttctgctctt
     1741 taccgctgtt tttatctttc gcggaaacgt gcaggatacc gtcagcatcg atatcgaagg
     1801 taacttcgat ctgcggcatg ccgcgcggtg ccgggttgat accatccagg ttgaactgac
     1861 ccagagattt gttatcagcc gcacgtttac gttcaccctg cagcacatgg atggttaccg
     1921 cagactggtt gtcttcagca gtagagaaca cctggctgtg cttggtcggg atagtggtgt
     1981 ttttcgcgat cagcgtcgtc atcacaccgc ccatggtttc gatacccaga gacagcgggg
     2041 taacgtccag cagcagtacg tcttttacgt caccagtcag aacaccaccc tgaacagcag
     2101 caccgattgc tacagcttcg tccgggttaa cgtctttacg cggctcttta ccaaagaact
     2161 cagcaacttt cttctgaacc attggcatac gagtctgacc accaacgagg ataacgtcgt
     2221 cgatatcaga tacagacagg ccagcgtcct gcagtgcaac tttcagcggc tcaatggaac
     2281 ggtttaccag atcttcaacc aggctttcca gtttcgcacg agtcactttg atgttcatgt
     2341 gtttcggacc ggtcgcgtct gcagtgatgt acggcaggtt aacgtcggtc tgctgagcgg
     2401 aagacagttc gattttcgct ttttctgccg cttctttcag gcgctgcatt gccagcggat
     2461 cgttgcgcag gtcaatgccc tgatctttct tgaattcttc aaccagatag ttgatcagac
     2521 ggctgtcgaa gtcttcacca cccaggtggg tatcaccgtt ggttgccaga acttcgaagg
     2581 ttttttcgcc gtcaacttcg tcgatttcga taatagaaat atcgaaagta ccaccaccca
     2641 ggtcataaac cgcgatagta cggttgccag tacctttgtc cagaccgtaa gccagcgcag
     2701 ctgcggtcgg ttcgttgatg atacgtttta cttcgagacc agcgatacgg cctgcgtctt
     2761 tggttgcctg acgctgagca tcgttaaagt atgccggtac ggtgataaca gcttcagtta
     2821 ccggttcacc caggtaatct tcagcggttt tcttcatttt tttcagcact tcagcagaaa
     2881 tctgcggcgg tgccattttc tggcctttaa cttcgaccca tgcgtcgccg ttatcagcag
     2941 caataatttt gaacggcatg atggaaacat cacgctgtac ttcttcgtcc tggaagcggc
     3001 ggccaatcag gcgtttaatc gcaaacaggg tgttttgcgg gttcgtcact gcctgacgtt
     3061 tagccggctg accaaccaga gtttcaccat cctgggtata ggcaatgata gaaggcgtgg
     3121 tgcgatcgcc ttcggcattc tccagcacac gaggagtggt gccatccata atcgctacac
     3181 aagagttggt agtacccaag tcgataccaa ttattttacc catctaaacg tctccactat
     3241 atattcggtc atcatgtggt tgtgagtctg taataggggc gaaacgtctg gtttcaactg
     3301 cccaatttca tttttttgca gactcactca ctgcggttga ctactaaatg gggtcgtaac
     3361 ccacgtcatc aagggggaga tgcaaaaatt ttttgtgctt ttgcgcagaa atttcgcgat
     3421 atggcaggac aagacgtaaa attgacagca attttattca tataaagaat gaatcgcagt
     3481 caatgtattc atcaaaaaat tgtaataaaa agaaaagatt acgtgcctga atcttctctt
     3541 tatcagcagt aaactagtgg gtattcatcc ccctacctct tcccactaag agaatcctta
     3601 tgaaatccgt ttttacgatt tccgcaagcc tggcgattag cctgatgctg tgctgcacgg
     3661 cgcaggcaaa cgaccataaa ctcctcggcg tcattgcaat gccgcgtaac gaaaccaacg
     3721 atctggcgct gaaacttcct gtttgtcgca ttgtgaaacg catacaactc tctgccgacc
     3781 atggcgattt acagttaagc ggtgcatcgg tttatttcaa agccgcccgt agcgccagtc
     3841 agagcctgaa tattccttca gaaataaaag aagggcaaac cactgactgg ctcaacatta
     3901 acagcgataa cgacaataaa cgctgcgtct caaaaatcac cttttccggt catacggtga
     3961 actcatcgga tatggccacg ctgaaaatta tcggcgacga ctaacgccag aatatagaag
     4021 ccacaaaaaa tgaatgttaa ttacctgaat gattcagatc tggattttct ccagcattgt
     4081 agtgaggaac agttagcaaa tttcgcccgg ctgctcaccc ataatgaaaa aggcaaaact
     4141 cgcctctcca gcgtactgat gcgcaacgaa ctgtttaaat cgatggaagg acatcccgag
     4201 caacatcgcc gcaactggca gctgattgct ggagaattac agcattttgg tggcgatagt
     4261 atcgccaaca aactgcgcgg acacggtaaa ttgtatcggg ccattttgct cgatgtttca
     4321 aaacgattga agctgaaagc cgacaaagag atgtctacgt ttgaaattga gcaacagtta
     4381 ctggaacaat ttctgcgtaa tacctggaag aaaatggacg aggaacataa gcaggagttt
     4441 ctgcacgcgg tcgatgccag ggtgaatgag ctggaagagc tgctgccgct gctgatgaaa
     4501 gacaaattat tggcaaaagg tgtgtcgcat ttgctttcca gccaattgac ccgcatttta
     4561 cgcacccacg cagcaatgag cgtgcttggg catggtttgc tgcgcggcgc gggtctggga
     4621 ggccctgtgg gcgcagcatt aaatggggtt aaagcggtca gcggcagcgc ctatcgcgtg
     4681 acgattccag ccgtactgca aatagcctgc ctgcgccgga tggttagcgc cactcaggtc
     4741 tgataccgga aaccggggaa aattttcatt ttcccctcaa aagatcgtag acactgcccc
     4801 actggctgat tattatgccg cgccctgaaa acactacagt tattcaggga aattatttca
     4861 ccattcattc gatgatgatt tttgaggaat tatgggcaac actaagttgg ctaatccggc
     4921 accgctgggc ctgatgggct tcggcatgac caccattctg cttaacctgc acaacgtggg
     4981 ttatttcgct ctggacggta ttattcttgc catgggcatt ttctacggcg gcatcgcgca
     5041 aatttttgcc ggtctgctgg agtacaaaaa aggcaacact ttcggtttaa ccgcattcac
     5101 ctcttacggt tctttctggc tgacgctggt tgcgattctg ctgatgccga aactgggtct
     5161 gaccgatgcg ccaaatgcac agttccttgg tgtctacctg ggtctgtggg gcgtatttac
     5221 gctgtttatg ttcttcggca ctctgaaagg cgcacgcgtc ctgcaattcg ttttctttag
     5281 cctgaccgtg ctgtttgccc tgctggcgat cggtaacatt gccggtaatg ccgcgatcat
     5341 ccactttgcc ggctggattg gtctgatctg cggtgccagc gcaatctatc tggcgatggg
     5401 tgaagtactg aacgagcagt ttggtcgcac cgttctgccg attggtgaat cccactaatt
     5461 gtcttgagat ccccccgctc ggggggattt ttttattcgc taacgtcgcg tcttgcactc
     5521 ttcggtctga atgcagcaac cacttctggt gccgtttcaa cgtatggccc ttccagcaac
     5581 tgaatgcagt acggtacgct ggcaaaaata ccgtgtacca caacgttacc ctcagcgtcc
     5641 ttcacacctt ccagcgtctc tttaatagac ttcggctgac cgggtaagtt aaggatcagc
     5701 gcctgtttgc gaatcacacc cacctgacgc gaaaggatcg cagttggtac aaaatgcagg
     5761 ctgatctggc gcatctgttc accaaagcca ggcatctcgc ggtccgctac tgccagcgtc
     5821 gcatcgggcg ttacgtcacg acgcgccggg ccagttccgc ccgtggtgag caccagatgg
     5881 caactcattt catccaccag ctcacacaac gtttgctcga tgatcgcctg ctcatcgggg
     5941 attaagcggg tttccagttc aaacggcgtg gttagcgccg atgtcagcca ttcttccagt
     6001 gcagggatgc ctttatcctg ataaacgccg ctggatgcgc gatcagagat ggaaactaag
     6061 ccaatacgta aagtattcat caagattttt ccgtttaaac agtctcgtta aacagaatga
     6121 tacactgcga agggagtgac agacaggctt cagaagaggt agcgtgaccg gcttcccggt
     6181 cacgctaaga atgattacag cagatcgccg atcatttttt ccagtttttc ctggtcaaca
     6241 gcaaacttac ggataccttc cgccagttta tctactgcca ttggatcctg gttgtgctgc
     6301 cacaggaact cggactcagt gatacgcgcc ggacgcgctt tcacttcgcc ggtgtaagac
     6361 agtttacgtt cgatagcccc ttcgctctca gccagctctt tcagcagtgc cggtgcgatg
     6421 gtcagacggt cgcagcctgc cagttccaga atttcgccga tgttacggaa gcttgcgccc
     6481 ataaccacgg tttcataacc gtgctctttg tagtactggt agatttcaga tacagaaacc
     6541 acgcccggat cttctgccgg agcgtactct ttcttatcgg tattcgcttt gtaccagtca
     6601 agaatacggc caacaaatgg agagatcaga aacacgcccg cttccgcaca agcacgagcc
     6661 tgagcgaagg agaacagcag ggtcaggtta cagttgatac cttctttttc cagctgttcc
     6721 gcagcacgga taccctgcca ggtagaagcc agtttgatca gaatacgatc gttgctaata
     6781 cctgcatcgt tgtagagttt gatcaggcgt tttgctttcg caattgacgc ttcggtgtca
     6841 taggaaagac gcgcatcaac ttcagttgag atacggcccg gaaccagttt caggatttcc
     6901 agaccaatat ttactgccag tttgtcggtc gcgtccacga tctgctgcgc gcgatcgttg
     6961 ctctgctgtt tcgcccaggc gacagcatca tcaatcaact tacgatattc cggaatctgc
     7021 gctgcgttaa gaatgagaga agggttggtt gtggcatcct gcggttgata cagcttcatt
     7081 gccgcgatgt ccccagtgtc ggccactacg gtggtgtact gacgaaggga ggtcaatttg
     7141 tccgtcatga tagtatttct ctttaaacag cttgttaggg ggatgtaacc ggtctgccct
     7201 gatgatatca cgacgcaacg tcagcgcaac cgccgacaag acggttatcg ccaggcgaga
     7261 ctgtttcgga tttctgacac tgcgttgata tcgcccgcca tttttaaaca aaacctcatg
     7321 tatgctacgc agaagttatc aagtacctcg tagcgtatat acttcttaaa caattggtaa
     7381 cgtttacaca ggaaagtcat cgcgaccggc aataagaggg atatgcatgc cagatttttt
     7441 ctcctttatt aacagcgtcc tttggggatc ggtaatgatt tacctgctct tcggcgcagg
     7501 ttgttggttc acttttcgca ccggatttgt gcagtttcgc tacatccgcc agtttggcaa
     7561 aagtcttaaa aatagcattc atccacagcc aggcggttta acctcatttc agtcattgtg
     7621 taccagtctt gcggcgcgcg tgggtagcgg caacctggcc ggcgttgcgc tggctattac
     7681 cgccggtgga cctggagccg tcttttggat gtgggttgca gcgtttatcg gcatggcgac
     7741 ctcgtttgcc gaatgttccc ttgcacaact ttataaagaa cgtgacgtca atgggcagtt
     7801 tcgtggcgga ccggcatggt atatggcgcg cgggctgggg atgcgctgga tgggcgttct
     7861 gttcgccgtc tttttgctca tcgcctacgg cataattttc agcggagttc aggcaaacgc
     7921 tgttgcccga gctctgagtt tttcttttga ttttcccccg ctggtgacag gcattattct
     7981 tgctgtcttt gctctgctgg caatcattcg cggtcttcat ggcgtcgccc ggctcatgca
     8041 ggggtttgtc ccgttgatgg cgataatctg ggtactgacc agcctggtga tttgcgtaat
     8101 aaatatcggg caacttcccc acgtcatttg gtctattttt gagagtgctt ttggctggca
     8161 ggaagcggca ggcggcgcgg cgggatatac cttaagccag gcgattacta acggttttca
     8221 gcgcagtatg ttttccaatg aggcgggaat gggttcgacg ccaaacgcgg cagcggcagc
     8281 ggcgtcctgg cctccgcatc cggcagcgca aggaattgtc cagatgattg gcatttttat
     8341 cgacaccctg gtcatctgta cggcaagcgc catgctgata ttactggcgg gtaacggcac
     8401 aacctacatg ccgctggaag gtattcagct tatccagaag gcgatgcggg tgctaatggg
     8461 ttcctggggt gctgagtttg ttaccctcgt ggttattctg tttgccttca gctccatcgt
     8521 tgccaactac atttatgccg aaaacaatct cttcttttta cgcctgaaca accctaaagc
     8581 gatctggtgt ttgcggatct gcaccttcgc aacggtcatc ggcggcacct tgctaagtct
     8641 tccgctgatg tggcaactgg cagatatcat aatggcctgc atggctatta ccaatttgac
     8701 cgccatttta ctgctctcgc ctgtggttca taccattgcc agtgattatc tacgccagcg
     8761 taaactcggc gtgcgcccgg tgtttgatcc gttgcgttat ccggatatcg gtcgccagct
     8821 ttctcgggac gcgtgggatg atgtttcgca ggagtaatca caactatcga tcaactcatt
     8881 ctcttttttt gctaaagtcg gcataaattt cctgcaagga ctggatatgc tgattcttat
     8941 ttcacctgcg aaaacgcttg attaccaaag cccgttgacc accacgcgat atacactgcc
     9001 ggagctgtta gacaattccc agcagttgat ccatgaagcg cggaaactga cgcctccgca
     9061 gattagcacg ctgatgcgta tcagcgacaa actggcaggt atcaacgccg ctcgctttca
     9121 tgactggcag ccagatttca cgccggagaa tgcccgccag gcgattctgg cgtttaaagg
     9181 tgatgtctac accggcttgc aggccgaaac cttcagcgaa gacgatttcg attttgccca
     9241 acagcatttg cgaatgcttt ccggcttgta tggcgtactc cgcccgctcg atttaatgca
     9301 gccttatcgt ctggaaatgg ggatccgtct tgagaatgcc cgagggaaag atctgtatca
     9361 attctgggga gatatcatca ccaacaagct gaacgaggcg ctcgcagcac aaggcgataa
     9421 tgtggtgatt aatctggcgt cagatgagta ttttaaatcg gtgaagccga agaaattgaa
     9481 tgccgagatt atcaagccgg tgttcctcga tgagaagaac ggcaaattta agataatcag
     9541 cttctacgct aagaaagcac gcggtctgat gagtcgtttc attattgaaa atcggctgac
     9601 caaaccagag caactgactg gttttaatag cgaaggttac ttctttgatg aagcttcctc
     9661 cagcaatggc gaactggtgt ttaaacgcta cgagcagcgt taagtttgta gtcaatgccg
     9721 gatgcggcgt gaacgcctta tccggcctac aaaagcatgc agatttaata tattgcagaa
     9781 ttcacgtagg cctgataaga cgcgccagcg tcgcatcagg cgctgaatgc cggattgtta
     9841 cccggcattt gtcatttagc ggtgatgttt gcctggacca tgaccgccgt gatgatcatg
     9901 atgagctttc ttatggtgac gcggcggtgg cggcggtccg tgtgggtgcc agcgattgcc
     9961 tcgccattca taatgttgtt tccaccagcc gtggtcgcgc cagtgaccgc catcccagta
    10021 atagccacga ttatcacgat cgcctatctg taattttact gacgggacta acgtaatttc
    10081 cgcagcctgc gttgccatgg gagcgaccag aaccagggaa agtgcgagta cgatagattg
    10141 catctttttc acaggttact ccttttatca ttgcccgtta tgggtcgata tccggagatt
    10201 acgcgatgag aacggcggtt gttattctcc gcaatcctaa tctctaagtt accgcattta
    10261 ttgagaattt ctcctttttc cctgtcgttt ctctccatat tttcttcaca aaaaagccgg
    10321 gctgcataaa agcaaatccg gcctgattga gataatgaat agatgttact gatgattcat
    10381 catcaattta cgcaacgcag caaaatcggc gggcagatta tgcgaaagca agggtaaatc
    10441 ggcacgttct gccagctctt ttggcagatc caacgtttca ccgagaatcg cttccacgct
    10501 ctctttaaat ttcgccggat gcgcggtgcc gaggaacaag ccatattcgc ctggattcaa
    10561 ctggtcacgc agcgcacgat acgctacggc agcgtgcggc tccgaggtgt agcccagttc
    10621 ttttaactca cgcattgtct gttgcgtggt ttcatcatcc acggctgcat aacccagctc
    10681 tttcagttgc cagattttgc ggcggaacaa ctcttccaca cgcggccagt tgttcggctg
    10741 actcacgtcc atcgcgttgg ataacgtcgc ctgagtcgct ttgggtgacc actgaccgtc
    10801 gtgcaggaaa cgtggcacgg tatcgttcac gttggtcgca gcaataaaac gtttcaccgg
    10861 cagaccgagt gacttcgcca gcagacccgc cgtcaaatcg ccgaagtttc cgcttggcac
    10921 cgagacaacc agctggttgc gcgcttcctg cggcagctgc gcaactgctt caaagtagta
    10981 gcaaatctgc gccagcaacc ggctgatgtt aatcgagtta gctgagttta accccagcgc
    11041 cactttcagc tcttcatcat caaacgcctg cttcaccagc gcctgacagg catcgaaatc
    11101 gccgtcgatg gcaacagttt cgatattgcc gcccaatgta cagaacagtt tttcttgcag
    11161 tggactgatt ttgcctcgtg gatagaggat aaccactttc acattcggta aaccgtagaa
    11221 agcatgagcc actgccgctc cggtatcacc ggaggtcgcg gtcagaatgg tcactggctt
    11281 atcgcccgca atatgggtca gcatttgtgc cataaagcga ccgccgaaat ctttaaatgc
    11341 cagcgttggc ccgtggaaca attccagaca accgacatcg ctttcaacat tggcgaccgg
    11401 agccgggaag gcaaacgccg cgcgcacgcg ctcttccagg atttcctgcg ggatttcatc
    11461 accaataaac gccgagagga tcttcgcact gcgggtgaca aaatccagct tcagcatctc
    11521 atcaatttca gtcaggctga attccggcag gtcgtgcgga aaaaacagcc cctgattttt
    11581 gcccaacccc tgggttacgg cttgcgcaaa gctgacctgc tcattgtgat ctttcagatt
    11641 gtagagtttc atttagtttt ccagtactcg tgcgcccgcc gtatccagcc ggcaaatatg
    11701 aacaaaacct tcctgatttt gcaggtagtt cttacccaac cagtcggcaa cgcgctgggc
    11761 ggtatccggc ttgtcacaca gagcgaacaa ggtcgggccg gagccggaga taccgctcgc
    11821 taccgcgccg atttccgcga ccgcctgccg cgcctgccgg aagccaggca gtaaccgttc
    11881 acggtagggt tctgcgataa catctttcat cagcttcgcg gcaagctcag gctgacggga
    11941 atagcaggcg tgaatgaagc ctgccagatg tcgcccgtgc gcaatgcaat cctggcggcg
    12001 atactgcgcc ggtaaaatag cccgggcttc tgccgtcgag actttaatcc ccggatacgc
    12061 cagcacccac agccactcat caaaccctgg cacttgctgg ctgatgatgt cgttttcttc
    12121 gatcatcaac tgcataccac caagaaaaca cggtgccacg ttgtcgtaat gaatgctgcc
    12181 ggagatacgc ccttccagct cgcccatcaa agccagcaaa cgggtgtcat taagcggctt
    12241 gccgcagtgt tcattcatcg ccatcagcgc cgcgaccacc gaacaggcgc tggagcctaa
    12301 gcccgaaccg atcggcatat tcttttccag agtcatcgcc actggaattt gcttgcccag
    12361 ctcctggcaa aaacgctccc agcactgata aacgatattt tcccgtggtt ctgacggcag
    12421 cttatcggca aagcgtccga ggttgttgag actgaatgtc tctgccgcct caaccgttac
    12481 tacatctccg agcaatgcac catcaacggg tgtcaccgcc gccccgagca catcaaaccc
    12541 gacgctcata ttggcactgg aagccggggc ataaacttta accatgtcag actcctaact
    12601 tccatgagag ggtacgcagc agatcggcaa agacaccagc agcggtaacg tcattgcccg
    12661 caccatatcc gcgcagcacc aatggcagcg gctgataata gtggctatag aaggccaggg
    12721 cgttttcgcc atttttcact ttgaacagcg gatcattacc atccacttcg gcaatcttca
    12781 cgcggcagac gccatcttca tcaatattgc caacatagcg caaaactttt ccttcatcac
    12841 gggccttcgc cacgcgcgcg gcaaagagat cgtcgagctg tgacagattc gccataaaag
    12901 cggcaacatc accctcggcg ttaaactctg cgggcagcac aggttcaatt tcaatatccg
    12961 ccagctccag ttcacgtccc gtttcacgag cgagaattaa tagtttacgc gctacatcca
    13021 taccagaaag atcatctcgc ggatccggtt cggtataacc catttcccgc gccagcgtag
    13081 tcgcctcgga gaaactcatg ccttcgtcta acttgccgaa gatataagaa agcgaacctg
    13141 aaagaatgcc ggagaacttc atcaattcat caccagcatt gagcagattt tgcaggttct
    13201 caataaccgg taatccagcc ccaacgttgg tgtcatagag gaatttacgt cgcgattttt
    13261 ccgccgcata acgcaactga tggtagtaat ccatcgacga ggtgttggcc tttttattcg
    13321 gcgtgacaac gtggaaacct tcgcgcagga agtcggcata ttgatccgcc actgcctggc
    13381 tggaggtgca gtcaacaatg accgggttca gcagatgata ttctttcacg aggcgaatta
    13441 agcgcccgag attaaacggc tctttggctt gcgccagttc ttcctgccag ttttccaggt
    13501 ttaggccatg cacattggta agcagagcct tcgagttggc aacgccgcag acacgtaagt
    13561 caatatgttt attcttcagc cagctttgtt gacgcttcag ttgctccagc agcgcaccgc
    13621 caacgccacc gacgccaatc acaaacactt cgataacctg atccgtattg aacagcatct
    13681 gatgagtaac gcgcacgcca gtggtcgcat catcgttatt taccacgaca gagattgagc
    13741 gttcagaaga tccctgagca atggcgacaa tgttgatatt ggcgcgggcc agcgcggcaa
    13801 agaatttcgc cgagatccca cgcaaggtgc gcataccatc acctaccacc gagataatgg
    13861 ccagccgttc cgtcaccgcc agcggctcca gtaagccttc tttcagttcc aggtagaact
    13921 cttcctgcat tgcccgttca gctcgcacac agtcgctttg cggaacgcag aaactgatac
    13981 tgtattcgga agatgattgc gtaatcagca ccacggaaat acgggcgcgt gacattgcag
    14041 caaagacgcg cgccgccatg ccgaccattc ctttcatccc cgggccggaa acgctgaaca
    14101 ttgccatgtt attcagattg gaaatgccct tgaccggtaa ttcgtcttca tcacggctgg
    14161 caccaattag cgtacctgga gcttgaggat ttccggtatt tttaatcagg cacgggatct
    14221 ggaactgggc gatgggggta atggtgcggg ggtgaagaac tttagcgccg aagtaagaaa
    14281 gctccatcgc ttcctggtag gacatcgatt tcaacaacct cgcatcgggc acctgacgcg
    14341 ggtcgcaggt atataccccg tcaacgtccg tccaaatctc gcaacaatcg gcgcgtaaac
    14401 aggcagccag caccgccgcg gagtagtcgg aaccgttgcg tccaagtacc accagttcgc
    14461 ctttttcatt accggcggtg aaacctgcca tcagcaccat gtgatcagcc ggaatacgac
    14521 ttgccgcaat acggcgggtg gactctgcaa tatcgacagt agattcgagg taatgcccca
    14581 ctgccagcag tttttcgacc ggatcgataa cggtaacgtt gtgaccgcgc gcttctaata
    14641 cgccggccat aatggcgatc gacattttct cgccacggca aatcagcgca gcgttaatgc
    14701 tatccgggca ctgccctaac aaactaatgc catgcaggac atgttttatt tgagcaaatt
    14761 cctggtcgac gaaagttttc aattgcgcca gcgggaatcc cggctgggcg gcggcgagtc
    14821 ccgtcagaag ttcggcaaaa atacgttcgg catcgctgat attgggtaaa gcatcctggc
    14881 cgctaatggt tttttcaatc atcgccacca ggtggttggt tattttggcg ggggcagaga
    14941 ggacggtggc cacctgcccc tgcctggcat tgctttccag aatatcggca acccgcagaa
    15001 aacgttctgc atttgccact gatgtaccgc cgaacttcaa cactcgcatg gttgttacct
    15061 cgttaccttt ggtcgaaaaa aaagcccgca ctgtcaggtg cgggcttttt tctgtgtttc
    15121 ctgtacgcgt cagcccgcac cgttacctgt ggtaatggtg atggtggtgg taatggtggt
    15181 gctaatgcgt ttcatggatg ttgtgtactc tgtaattttt atctgtctgt gcgctatgcc
    15241 tatattggtt aaagtattta gtgacctaag tcaataaaat tttaatttac tcacggcagg
    15301 taaccagttc agaagctgct gtcagagact ctttttttaa tccacacaga gacatattgc
    15361 ccgttgcagt cagaatgaaa agctgaaaaa tacttactaa ggcgtttttt atttggtgat
    15421 atttttttca atatcatgca gcaaacggtg caacattgcc gtgtctcgtt gctctaaaat
    15481 ccccaggcgt tgttgtaacc agtcgaccag ttttatgtca tctgccactg ccagagtcgt
    15541 cagcaatgcc atggctcgtt cgcgtaaagc ttgcagttga tgttggtctg ccgttgtgtc
    15601 acttttcgtc ggttgttgta ttaatgttgc taattgatag caatagacca tcactgcctg
    15661 ccccagattg agcgaaggat aatccgccac catcggcaca ccagtaagaa cgtcagccaa
    15721 cgctaactct tcgttagtca gcccggaatc ttcgcgacca aacaccagcg cggcatggct
    15781 catccatgaa gatttttcct ctaacagcgg caccagttct actggcgtgg cgtagtaatg
    15841 atatttcgcc cgactgcgcg cagtggtggc gacagtgaaa tcgacatcgt gtaacgattc
    15901 agccagcgtc gggaaaactt taatattatc aataatatca ccagatccat gtgcgaccca
    15961 gcgggtggct ggctccagat gtgcctgact atcgacaatc cgcagttcgc taaaccccat
    16021 cgttttcatt gcccgcgccg ctgccccaat attttctgct ctggcgggtg cgaccagaat
    16081 aatcgttata cgcatattgc cactcttctt gaccaaataa ccgcgaacag ggtgatcact
    16141 gtcaacttat tacgcggtgc gaatttacaa actcttaacg taagtcgcag aaaaagccct
    16201 ttacttagct taaaaaaggc taaactattt cctgactgta ctaacggttt
//
LOCUS       DAZUCE010000108        16120 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZUCE010000108 DAZUCE010000000
VERSION     DAZUCE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.108"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(42..1580)
                     /gene="cadC"
                     /locus_tag="ACHY03_002914"
     CDS             complement(42..1580)
                     /gene="cadC"
                     /locus_tag="ACHY03_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFS3906121.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAIPATDSPSHSLNIQNTATLPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1840..1999)
                     /locus_tag="ACHY03_002915"
                     /pseudo
     CDS             complement(1840..1999)
                     /locus_tag="ACHY03_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            2335..3222
                     /locus_tag="ACHY03_002916"
     CDS             2335..3222
                     /locus_tag="ACHY03_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFS3906122.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLEMIVLSATESVVGMVNPVLPDVR
                     EFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKV
                     LPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTIHSLACELLPEW
                     RAFFDALLENGGKSLWAEN"
     gene            3555..3764
                     /locus_tag="ACHY03_002917"
     CDS             3555..3764
                     /locus_tag="ACHY03_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906123.1"
                     /translation="MCVGCGYSGVRDGAGKNALVWVKVNPESGLGLCSGCDSFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
     gene            4000..4902
                     /locus_tag="ACHY03_002918"
     CDS             4000..4902
                     /locus_tag="ACHY03_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906124.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQSVPGNSVNCMCWASLAALAFDAEERVERQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCTGRIQAGRHHSGSHPDVSVYTERCAQ
                     GMSLSPVHSARQCPVQPTDSAETE"
     gene            5250..6617
                     /gene="dnaB-PI"
                     /locus_tag="ACHY03_002919"
     CDS             5250..6617
                     /gene="dnaB-PI"
                     /locus_tag="ACHY03_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFS3906125.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVDYLQLIRA
                     PELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDG
                     PGGYA"
     gene            6617..8350
                     /locus_tag="ACHY03_002920"
     CDS             6617..8350
                     /locus_tag="ACHY03_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFS3906126.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGEPRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEEQLG
                     RTVSLRELADLLTHEGLPIHNSTISRMEDALKYLYPWIPDLMESGLGRPQVTALLALR
                     QDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHPS
                     LDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPD
                     TDTEAMPESTVSIFPGTEREQHGITRPLKAVSDTDTERTEKCSRPSQEESAGRGDSGA
                     SSAENALRPEIQPDMYGGKPVFSGDVMEDDAAGAVLASVMTGDTAPGTTDALPASVTH
                     FAVRRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFL
                     AGLAGDAPRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSVL
                     PDDDEGDENDE"
     gene            8343..9068
                     /locus_tag="ACHY03_002921"
     CDS             8343..9068
                     /locus_tag="ACHY03_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFS3906127.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGGRY
                     RRCVTFYGFSERPQVAAYAFDVLCRQLREATDAYLRTQSRRLKLSTRRARAAQFRDGW
                     VQGIRQVVMPFSVTEEESRLMRLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNA
                     SLHHGVGESARQVVPGLMIREAS"
     gene            9065..9673
                     /locus_tag="ACHY03_002922"
     CDS             9065..9673
                     /locus_tag="ACHY03_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFS3906128.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            9688..10107
                     /locus_tag="ACHY03_002923"
     CDS             9688..10107
                     /locus_tag="ACHY03_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFS3906129.1"
                     /translation="MAEINFQVLRENDAGELTGVATAGRPVARHFDDGLTLEVNRTCT
                     TGERNANSALYGAVWRAAKDMGYRRCITYTRADESGASLRAAGFVRVKELPSGKSWAE
                     SSVALKSTRDLVGSGGVPCVLREIRRMSTTVIHIKGE"
     gene            10111..10578
                     /locus_tag="ACHY03_002924"
     CDS             10111..10578
                     /locus_tag="ACHY03_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906130.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGVLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            10596..11840
                     /locus_tag="ACHY03_002925"
     CDS             10596..11840
                     /locus_tag="ACHY03_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFS3906131.1"
                     /translation="MTLPVDSLIAYTLEKMQERIVQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQRVTEGRMQ
                     PGSETEPGVETQSSVLKMLSSDSELSEKQGQKILSSDSEPSEKSEGYHRVRKPNHYVR
                     NITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELAQGVLESLSGMLGSHSLQ
                     NPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAE
                     RLSGIVAGLRASLRRCSAMRSE"
     gene            11952..12431
                     /locus_tag="ACHY03_002926"
     CDS             11952..12431
                     /locus_tag="ACHY03_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906132.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMFLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
     gene            13043..13747
                     /locus_tag="ACHY03_002927"
     CDS             13043..13747
                     /locus_tag="ACHY03_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFS3906133.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
     gene            13750..15792
                     /locus_tag="ACHY03_002928"
     CDS             13750..15792
                     /locus_tag="ACHY03_002928"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFS3906134.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLSRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGCLITAMKNASSGVT
                     DPALKKILRENAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALPDSLT
                     NPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLC
                     NGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSGDVGDKLN"
BASE COUNT         3947 a         3629 c         4670 g         3874 t
ORIGIN      
        1 aacaacaaat attcccgatc atgaatcaac ccgggggtta cttattctga agcgagaaat
       61 ttgtcgagat aaggtacaac ataaggaaca gaagtctgga atataccatt ttcaatccag
      121 taaagggtgt ttgcccctgg gcgtaaatta aaggcggtga gatatgcatc agccgcttcc
      181 cggttcatcc ccttcatttc ataaaccttg ccaagcaata cataatttag ccaggacatt
      241 tcaagatcaa tgccagtatt tatcgcctgg taagactcat ctgttttacc ttttaccaga
      301 gcactgaccg cttttatttg atatataatg gacaggttat tcaattccgg tagtgtaaca
      361 atgttatcta tttctgtgtt cagtgctgct aattgttttt catctaaagg atgttgagaa
      421 tggcgcacga tatcaactaa tgctttttct gctctcgcgt aggtaaattc tggggatgat
      481 tgaacaatct cacctaataa ttcactggca cggttcaatg atttatcatc gccatgcagt
      541 aaataatcat gtgcctgata aaaattagtt aataacgcac cacgatgagg caaaatttgc
      601 tggagcgtct cctgcattcg ttgtggccac ggttggttta acgcttttga taaactctcc
      661 agtaaatcat tttgaatcgc cagctgatta ccgttagtga tgacataacg tttatccagc
      721 atggttgaac catctgcatt gtctactaat tttatcgaca taaagcattg ttgagcacgg
      781 tattggcgct gattaacaaa cgcaatagat aatgttttac cggaactgct cggttcatca
      841 atgttgtagt tgattttgtc atgcaccata aaggtggaga aggtgttaag tgatgtcgcc
      901 accaaatcac ccacgcctat cgcgtaagag agctgatacg gggaactcca gctgttacaa
      961 cttttattta ccatattaat gtcaatatcg cgtggattga gcaaaatacg cgatttgctc
     1021 atcggaagac gtgtatcaag acttgaaaac gctaccagtg ctacacagat acctaacgac
     1081 aacaggaaaa aaaaccatac ccaaaaggta gtgaatcgtt tgcttttaac tggggattgt
     1141 tcaggtagcg ttgcggtgtt ttgaatgtta agactgtggg agggagaatc tgtggcagga
     1201 atcgcctctg gtataggggg aggcgaagat agcattattt cctctccctc ttcttcgctg
     1261 taccagataa ccggcaccat taatttatag ccgcgctttg gtacagtagc gatatagaca
     1321 ggactatctt catcattatc ttttaatgac ttacgtagtt ctgagatact ctgcgtcaca
     1381 acgtgattgg tgacaatact tctcttccag acattatcga taagttcatc cctgctaagt
     1441 acttcgccac tgtgttgagc aaagaaaacc agaaggtcga ttaatctcgg ctcaagggta
     1501 agttgacgcc cattgcggct aatttggttt atggacggag taacaagcca ttcgccaacg
     1561 cgaactacag gttgttgcat aatagaaact cattcgaaaa gggaatgatg caatgataat
     1621 tgccacaacc tatttttacc atctatagat gggtttattt acatattatt ggtgaatgca
     1681 agacgttatt tttaccagcc ataaacttct tgattacata gtattacgaa aggattttac
     1741 tgagaaccag aagtaatatt ccttaccatc aaaattcatc atctttgcca aagaaaaatg
     1801 ttcagaaaat aatccatgga aaattgtccg gagcgcttac tattttaatg gattgttagt
     1861 ctttgcatga gcaagcggac tgatacattt ctctttgttc tcattcagaa aatctcatca
     1921 gtcgccgttc agaccggtgt tgtgctttat ggatgcgtta cgctcctgat gacgtcattt
     1981 gacgttcaac agcatcacgg ggtcgcacga catttcacgt cagttagtgc tatcgctcag
     2041 gaacaaattt tcccgaattg ggatatgccc gcaaattgct ggtgatgtgg ggagaatctg
     2101 gttgagttcg gtagaattga tttggaggca gaacgcttaa atcgtggcgt cttgaaacga
     2161 aaaacggacc tccgtggagg tccgtttata tgaatttggt gccggactcg gaatcgaacc
     2221 aaggacacgg ggattttcaa tccccgtgtt gcgagtattc aggtagtgaa gtgaatattc
     2281 tggctggttt ccgtagtgta agtgattggt tgcatacttt aaagagtgtg ctatatgaaa
     2341 attttaccgg ttatatccac taaaggtggc gaaggaaaat caacccagtc tgctaatctt
     2401 gccggttttc tggcagatgc cggcctccgt actctgctga tagacggaga ccatgcacag
     2461 cccacggcaa gcaatatctt tccgctgacg tatgaagcgc cggccggtct ttttgaatta
     2521 ctgatgcgca cggctgacct cagtcatcct gacaacatca tttcccgtac ggctgtcagg
     2581 gggcttgacc tcatcgtctc caacgacccc catgagcaac tgaaaacggc catgcttcat
     2641 gcgccggatg gtcgccttcg tctgcgtaat gtgcttcagc atccgctttt tcagacctat
     2701 gacgtcattg tggtggattc acagggcgca cgttctgtca tgctggaaat gattgtgctg
     2761 tccgccacgg agtctgtggt ggggatggtg aatccggttc tgccggatgt gcgtgagttc
     2821 atccgcggca cggtcaatgt catggaaaac cttatgccct accgtgaact gggtattccc
     2881 ctgccgaaag tccgcaccct ggttaactgc atggattata cggcgctggc cagacagaca
     2941 ctggcagagc tgacaggcat tatcaacgcc ggtcgctaca gcaaagtgct gcctgaaggt
     3001 gcggtcaccc ttctttcaac acagatttac gacctgaata tttataaaca ggggcacgca
     3061 gccggtcagc cggtgcatcg tctggaaaaa gtgtctgccc gttgcagcga ttctgcgctg
     3121 gtcaccatac acagtctggc ctgtgaactg ttgccggagt ggcgtgcgtt ttttgatgct
     3181 ctgctggaaa acggagggaa aagcctgtgg gcagaaaact gatatggcgt gccgtggctt
     3241 cagcacccgg acacgaggaa gtgcatgtgt ttgttgatgc atctgaccgc gcagaagcga
     3301 aaaagcattt gtatcagcgt ctggcggagg aatggcgttg ttcagtgaac cggataactt
     3361 tccacaatat gtggaatgaa aacgaattac gggaaatgtc aacaggtccc cgggagcctg
     3421 caggactccc gctgcttgaa tccggagggg gtaacggcgg ggttttttat gaccatgagc
     3481 cgttgctcct tattgcctcc cggcgtctcc gcgcatcact cgaaactgca ctcatcgtac
     3541 tgaagaagtg gacggtgtgc gtgggctgtg gttattccgg ggtacgggat ggtgccggta
     3601 agaatgctct ggtctgggtg aaggtgaatc ctgagagtgg tctggggctg tgttccggtt
     3661 gtgactcctt tcttttgcag gaacgccagc gtcgtcagcg gcatgaccag cttttttcat
     3721 gtggtctttc ctgcaaacag cgaaagacgg agggggcggt atgacagtct ttgtaatgca
     3781 tatcccggtc ggtacagtta tgagcgcaaa gatgctgtcc gtaccttctg accagggtcg
     3841 ccaaacttac cggtctgaaa acgtacagac agttaccggg attttattcc cgacaactgt
     3901 tcgtccggca cccttttccc ttttttcata tagccaggtt tccggttctc tgcggggaag
     3961 tgacgcacct ggcgggctga gagatatgga ggtgaaagta tgtcttcctc agtgaaatat
     4021 tctccggttc cggcggtctt aacccgccct gaattactgg ctgaactgaa cctgctgatg
     4081 acccggattg ccagtgggtg tcgtgtgggg cttccggcgt caggtcgtcc ggtcattgcg
     4141 gtgcacggtg gttatgtttc atacagttct ccacgcaccg gacatgtttt tcaggacggt
     4201 tcccgcccgg agacagtgat gaccgtcagt aaccgggaac ggatgtgcga atggttcagt
     4261 caggtctggc ttccgctgca tctggctgcc cttgaaggcg atgagaacgc ttttttcagt
     4321 gctgaggtca acatggtgcg ccgggagctg cagagcgttc cggggaattc agtgaactgc
     4381 atgtgctggg caagccttgc tgcactggca tttgatgctg aagagcgggt ggaacggcag
     4441 aaaggcaata atgagaacat cgcaacatat gatgagagca tcgcaacata tgcattccgt
     4501 gctgcctgca ctgttagtcg ctgtctgagc cggaatgctt ccggaggtat tccgcgctca
     4561 taccctgaat ggaaagcgat tttgtttccc cgtgatggtg ctgacatttt ccccgacggg
     4621 tggctggaaa atatgacccg tcgggctgaa cgtgttttta cgagtgcgaa acgtcagtgt
     4681 ggaatgctct cagccagtca ggcagcgctt ttttctcaac aaacacgtca gtgtgccagt
     4741 gagcaatgca ccggccgtat tcaggccggc cggcatcatt cagggagcca ccctgacgtt
     4801 tcagtttata cagaacgctg tgcacaagga atgtcgcttt caccggtcca ttcggcccga
     4861 cagtgtccag ttcaacccac tgattctgct gaaacggagt aaaaggatga gccgattcaa
     4921 tacagttcag gggaaagggg ttacagagcc cgtctttttc cgtcatgacc tttccgttac
     4981 tcagcagcca gaactgcagg gtgtatttca cttcagattc aatgccggac ggcagttgtt
     5041 tttctgtatt cataaacttt ttcctttaaa acaacttgtt atgaatgaac gaatgctgtc
     5101 cgtaccttca gaccgggggc ggcaaactca ccggtctgaa aacgtacaga cagttaccgg
     5161 gattttattc ccgacagctg tacgtccggc accctttttc cttttttcac gtagccaggt
     5221 ttccggctgt cagaggggga gggggcgcca tggctgaaat gtctgttctc tgcgcatacg
     5281 aagcagagca ggccgttctg ggcggtctga tgctggagaa tgaacgctgg gatgaagtgg
     5341 tgctgatgct gagtcctgac gatttttatg tggctcagca ccgggctatt ttccgtgcca
     5401 tgtctgaact ggcggtgaat ggccagccac tggacctgat tacactgtca gagcacctgg
     5461 aacatcaggg caggctggac gtactgggcg gctttgctta tctggccgag ctcagcaaga
     5521 acacaccttc tgcggccaac atcatggcct atgcaggcat tgtggtggag aaaagcgttc
     5581 tgcgtcagtt acaggccacc ggaaacaccc tgattgctga tgtggcggca ccgaacgcca
     5641 catcacggga tgtgctggat gctgcagagc ggcgtctgtt tgcacttgcg cagaacagga
     5701 cgctccggga acgcacggaa gcctctcttt catccgcgct tgataccgtt ctgcagcaac
     5761 tggaaagtgt gctcggctcc ggtggtgtta ccggtacccc cacgggattt gactgtctgg
     5821 atgaaatgac ctgtggcctg cagcctggtg acctggttct gctggctgcc cgtccgtcaa
     5881 tggggaaaac ctctctggcg ctatccatgt gtctcagcgc cgcactgcgg cgaacggaag
     5941 agagcgttct ggtattcagc attgagatgc cgaaggagca actgattctg cgcatgcttt
     6001 ccatgctggg gcgtgtggaa ctgaatcatt tgcgcagtgg caacatggat gatgaggact
     6061 ggctgagggt gtcatctgcg gtcggtatgg cgcttggtga cggaggaatg ggctctctgg
     6121 gtgagcgcat aataattgat gactgctgtg agcagacgcc ttcatcactg cgtgcaggcg
     6181 cccgtcgtta tacacgtctg tacggtaaac cctccctgat tatggtggat tatctgcaac
     6241 tgattcgtgc gccggagctg gaaaaccgca cacaggaaat tgctgaaatt tcccgttccc
     6301 tgaaagcgct ggggaaggag ctgggctgcc cggtgctggc cctctcccag cttaaccggc
     6361 aggtggagca gcgtgctgat aaacgcccca ataacggtga cctgcgtgat tccggggcgc
     6421 tggagcagga tgctgacctg attatgttta tttaccgcga cgaggtctat aaccccgact
     6481 cactggcaaa gggggaggca gaaatcattg tcagcaagca gcgtcagggg cccacgggta
     6541 cggtacgggt caggttcgac ggtcgttaca cactgtttac gccctctgag aatgacgggc
     6601 cgggaggata tgcgtgatgg cacataaacc actggatctc ggggcggcta ttatgcagcc
     6661 cggtcgtcag accactggtg ccggcaatgt ggtggcaatg ggggaaacgc ccatggttct
     6721 gacgctggac cagttacgtc cgaatccgga taacccgcgc accacgcgca atccgcgtta
     6781 tgacgatatc aaaaattcca ttcatgcccg cgggctggat acggtgccca aagtcacccg
     6841 tatgccggac agtgagccgg atgtgtatat cttcagtgac gggggaaaca cccgttacca
     6901 gatactgacg gaactctgga aagaaacagg ggagccgcgt ttttaccgta ttcatgtgct
     6961 gtttaaaccc tggccgggtc ggctccagtg tgtgattggt catctggctg aaaatgaagt
     7021 tcgcggtgaa ctgactttta tcgagaaggc gcagggggtc cacaaggcaa gacaaatcca
     7081 tgaggaacaa ctgggcagaa cagtgtctct tcgtgaactg gctgatttgt taacccatga
     7141 agggttgccg atacataact ccacgatcag cagaatggaa gatgccctga agtatctgta
     7201 cccctggatc cccgacctga tggagtcagg actggggcgt ccacaggtta cagcactcct
     7261 cgcacttcgt caggacgcag aacgtgtatg ggggcagttt gctgttgcag ccgatacgga
     7321 tgcggaattt gaccgcgtgt tcggggaaag ctgccggaag tttaattcgc cggaactgtg
     7381 gtcactggag atgttccgcg atgagcttat cggagatttg ttgcaggccc tgccacatcc
     7441 ctccctggac tatgaccggt ggttgctgga actggatccg aaagagcgaa accgtcgtca
     7501 tcattttggt gagccggaac cggtggtggt gcctccttca gtccgtggca gcagtacggt
     7561 acaacccggg gcaccggtat ccgggatccg gtctgaagta ccggagtgtc aaccggatac
     7621 ggatacagag gcgatgccgg aaagcacagt ctccatattc cccggtactg agcgcgagca
     7681 acatggtatt acccggccac tgaaagccgt ttctgatact gacacagagc gcactgaaaa
     7741 atgcagtcga ccttctcagg aagagagcgc aggcaggggg gacagtgggg cgtcttctgc
     7801 tgaaaatgcc ctgcgtcctg agatacagcc ggatatgtac ggtggtaagc ctgtcttcag
     7861 tggtgatgtt atggaagatg atgctgccgg ggcagtactg gcatcagtta tgacaggaga
     7921 tactgccccg ggaaccactg atgcacttcc cgcgtcggtg acgcactttg ctgtgaggcg
     7981 ggatgatatc gaacatctgc agaatgaagc gttccgtctg gcctgggaac tggctgaatc
     8041 agccggttgt gctgaagaga ttgccatgga cagggaaagc gacctgtctg ccggtttcgg
     8101 ggcagcagtg gagaaatgtt ctcctgtagt ggcgtttctg gcgggtctgg ccggtgatgc
     8161 accgcggaca gtcctccccg tgagtctgaa agacctgctt acagggggca gtgcaccgga
     8221 agcgtggcca ctgctggatg acgaccatgc cgtgaagctg ctgcgtcttc tgagtgtgct
     8281 gcgccgcctg cgagccctgc agcgcagtgt attacccgac gacgatgagg gggatgagaa
     8341 tgatgagtga tatgtcgcat caggaacgtc tgatcaggcg ggtcaggaaa ctgctggagc
     8401 tgagccgtaa taacagcaat gcccatgagg ccgggctggc gcttgcaagg gcgcaacagc
     8461 tgatggcgaa acacggtatc acggagcagg atgccggact ttcttcggtg cagacctctt
     8521 catcacaggg ggcaccttct gatgctgaaa aagttccggc gtggatgttt cgtctggtca
     8581 tggtggtgac acagacattt ggctgccggg cgtattacga ctggcgggcg gtgcccggag
     8641 gccggtatcg ccggtgtgtg acgttttacg gtttcagtga acgtcctcag gtggcggctt
     8701 atgcgtttga tgttctgtgc cgtcagctaa gggaagcaac ggatgcgtat cttcgtacac
     8761 agagccggag actgaaactg agcacccgtc gtgcccgggc ggctcagttt cgtgatggct
     8821 gggttcaggg gattcgtcag gtggtgatgc cattcagtgt gacagaagag gaaagccggc
     8881 tgatgcgtct ctggatggag cagcaggata tggtgacggt ggactcccgt gagatgaaag
     8941 cctgtcgcgg cggtgacctg gcccgctggc aggggtatga ggccgggaaa aatgcctcac
     9001 tgcatcatgg tgtgggggag agtgcccgac aggtggtgcc gggtctgatg atacgggagg
     9061 cgtcatgaat aacagcttgt ctcaggcaac caacggtctg ctgatgcaac tggtgatgga
     9121 cctcaagagc ggctacctgc gtcgctgcga atctctgggg ctggcccggg aggaaatgca
     9181 gatgctgcag ggactgacca ttgaggaaat tcattacctg tcaaactctg aagtgtcggt
     9241 tatccggctg gacattaatc atgaaaacct gctgcgtatg ctgcagcagg cccggacgga
     9301 gcagaaacgt ctgcagcgca ttgaccgggc actggcgctg ggaggctcca ttgaactgat
     9361 ggcgttttac tttggacttt ccagtgtgga tgtggcggcg cgccgtcgca ttgccggtat
     9421 tgatgttcgc ccgggacgtg gcatcacact gagtgatgag gaaagtgcag agctgtggcg
     9481 tttatggcag aagagcggca tcacggatgt ggagagtgcg gacggactgg atgtcatgat
     9541 gctggcagct gagcaaatga atatcccgct gacggcggta tggcatgccg tgaggggatg
     9601 gcatcaggga actgcggggt ttaatgatgt atcggccagt cgccggaaac tgaaccggga
     9661 aaaagcagaa tgacaaggac aaatgacatg gccgaaatta actttcaggt actgcgggaa
     9721 aatgatgcgg gtgaattgac aggagtggcg acagctggca gacctgttgc ccgccacttt
     9781 gacgatgggc tgacactgga ggtcaatcgt acctgtacta ctggtgaacg taacgctaac
     9841 agcgcacttt atggcgctgt ctggagggcg gcaaaagata tgggctatcg ccgttgcatt
     9901 acatacacca gggctgatga atccggagcc tctcttcggg cagctggttt tgttcgtgtg
     9961 aaagaacttc cttcaggaaa aagctgggcg gaatcaagtg tcgccctgaa gagtacacgc
    10021 gatctggtcg gaagtggtgg tgttccttgt gtgctccggg aaatcaggag aatgagcact
    10081 actgtcattc acatcaaagg ggaatgagat atggctattg cagagagaac gggtatctcc
    10141 tcgcgcggcg atacttacga aatctggttg ggagcaaact gttatcacta ccgctctgat
    10201 ggttatgcag tgcttctgaa agaaggcagg aatcgtgctt atccatgtcg ggtgacgagt
    10261 gaaacgatta aatctgcgct ggcagcaatt gttgaattgc aacagaaatt aactgcggca
    10321 gagaagaaaa aggagtgtga gaaccacggg gttcttttgc cagctgagat gttaattcgc
    10381 tgtctggaag atcaaacagg gggacggtgg atccgtgcac atgaaccagt aatgctgatg
    10441 aaacgattaa tcagtaacct gaaaaaagcc agaccagaat gtaccgttcc tgatgaagcg
    10501 ctggcatatc tccggcgtaa ctgtctggtg aatatgggcg atatacccgt taccagcaga
    10561 cggatgtctg acgtataaca aaaaggaaaa gtgtaatgac attacccgta gacagcctga
    10621 ttgcgtacac gctggaaaaa atgcaagagc ggatagtgca gaaaggccga caacaggacg
    10681 gaaaaataca aagcggactg ctgtttaccg gaaatgtgca tgatgccatt ccgcgtcgac
    10741 tgcttctgga cacgcgactt tctccgctgg ataagatggc gtggatgatg attcggctgt
    10801 atgcacagaa taatgaaggt gcagttttcc ccacatatga cgaactgcaa ctgcagcttg
    10861 cttccccggg gaaaggaaaa gcctccaggg agactgtcag ccgggtgctg ctgatgctgc
    10921 ggatcacggg ctggctgagt ctgtgcaaac gggtgcgtga tgagaaaggg cgggtacggg
    10981 gcaatattta tgcccagcac gatgaaccgg tgacgtgccg ggatgctgaa atactggatc
    11041 cacgttatct ggatatggtg gcagatgcct gtatgagccg gaaccggacc ataagtcaga
    11101 cggcccggga tattctgtgc gaaataaaaa atgatccggg aatgcgtcat tatcacagcc
    11161 atatcagtca actggaagcc cggataggga gtgcccagac accgggacag atggcggccc
    11221 gtcagcgggt gactgagggc agaatgcagc cgggttcgga aaccgaaccg ggggtagaaa
    11281 cacagagttc ggtattgaaa atgctgagtt cggattccga actcagtgaa aaacaggggc
    11341 aaaaaatact gagttcggat tccgaaccat cagaaaaatc agaaggttat cacagagttc
    11401 ggaaaccgaa ccattacgta cgtaatatca cacacagtgt gattaaaaac acgtacgtat
    11461 tgcctgaagc gctgacaggg caaatcaggg aggaggaggc cattatgctg accagtcagt
    11521 tgcaggcgct gccggaagaa ctggcgcagg gggttctgga aagtctgtca gggatgctgg
    11581 gcagccactc gctgcagaat cctgtgggct ggttgcttgc agtgctgaag cgagcccggg
    11641 agggaaattt ttatccgccg aagcagaaac aacgtgttgt tgtgccagcc tccgtactgg
    11701 caggagaaag ggggcgagct actgcgcaat cagtttcatc ggggaaacgt actgaaccga
    11761 tttcagcaga acgtctgagt ggcattgtgg ctggactacg ggcctcgctg cgcagatgtt
    11821 ctgcgatgag atctgagtga ttgcaatacg ggctatgttc gcgggaacaa aaatcaacca
    11881 gtgccgccag cgactggtta aaaagaaaac agaatcggga actggtcagg tgaaacaagg
    11941 tggtaaatag catgcagaaa tacaaacagg aaagtctgag tgcagccatg aaagaaagtg
    12001 gcctgttgat cgctaaaatt tgtgcctgcg tgattgccgg agtggtggtt atctgggggc
    12061 tggtgacagg cacagagacc ctgctcactg cgctgaaaac aatgcacgaa cagtacgata
    12121 acagtatgtg ttatatcgta tgtgccacag ggtggttttt cctggtgtta agtaactggc
    12181 tgtgggtcac atacagtctt tataaagcgc gtacaggcag tacagtcgga gggggcctgc
    12241 aatttatgtt cctcctgagt ctgctggcag aatgcttttc attttttgtg attttctggt
    12301 cattgtctca tctggatata accgacattc agttgctcgt gatggggata acgacattaa
    12361 tgctgttttt gtctctgaac gttaaggtag tagtcatcat taaccatgtt gccagtggca
    12421 acagtgatta aaaaatgagc ggtgttgcca gtggcaacag aggttaaaaa atgagcagtg
    12481 ctgccagtgg taacggtgat taaaaaatga gcggtgttgc cagtggcagc agagattaaa
    12541 aaatgagcag tgctgccagt ggcagcagag attaaaaaat gagcagcgct gccagtggca
    12601 gaagaggtta aaaagcgaac agtgctgcca gtggcagcag aggttaaaaa gcgaacagtg
    12661 ctgccagtgg taacggtgat taaaaaatga gcggtgttgc cagtggcagc agagattaaa
    12721 aaatgagcag tactgccagt ggcagcagag attaaaaaat gagcagtgct gccagtggca
    12781 gcagaggtta aaaagtgaac agtgctgcca gtggcagcag aggttaaaaa gcgaacagtg
    12841 ctgccagtgg cagcagaggt taaaaagtga acagtgctgc cagtggcaac agaggttaaa
    12901 aagtgagcgg tgttgccagt gtcaacagcg attaaaaata atcggcgtta aatgtatatt
    12961 ctgcctgatt tgtttttttg ttgtcggtaa tacgcaacct gcggactatc ttctggtcat
    13021 gaaatcattt ggagtaaaca caatgcccca ggagaaaacc ggagaagtca gaaaatcaga
    13081 acaagttggt gctctgcagt catcgctgac tattgccctt catacccgat atgcgatccg
    13141 tctctgggag ggacgcagga ataatcagaa ggaggaaaaa caggaaaagc ggcctgacat
    13201 catcagtatg cccagggcta ttgcgtttgc gggtcaggca accagagatt cagccaggga
    13261 taatccttat gcagatatga tgttagtcag gctggaaaat gccctgacga aggcaactga
    13321 aacaattgaa aagcatcttg cgtggcttga cgggatcctt aaaaacctgc cgggacagat
    13381 ctccctttct gatattaaat cctcttcgcc ggtaaatatt ggcgtgtaca gtagttctcc
    13441 ggtcggttat cgatgtgtat ggcttctgat cggatatgat cgactggtca tgaaggtatt
    13501 tcaggtattt cattacggac ttatttcccg cacaaaacgg gatgagttgt tgaatcaggg
    13561 agggtatgcg gtacgcagga tttacgggat tattcaaaat tataaatcag tcagtgttac
    13621 acgtgctgat attttgtccg gcacagcccg tggaaaggag gcggttaacc gcatggggga
    13681 gccggatcct gacgtgatga gcggtaagaa acgttctgtt tattcaccac cggttaaagc
    13741 ggggtgagta tgcaactctt tctgtgtgaa aaaccttctc aggcgaaaga tatcgcccgt
    13801 gaactggcgc tcagtaagcg gggcgacgga tacatcggag atgacaatat tgctgtcacc
    13861 tgggcgatag gacacctgct ggaactggct gagccggaag cttacggtga acagttcggt
    13921 tccccatggc gttctgatgt gttgccgctg ataccggaac aatggcagat ggtggtgaag
    13981 ccgaaggtga aaaaacaact tattgtgatc cgggggctgt taaaacgcgc ttctgaagtg
    14041 gttattgcca cagatgccga tcgtgaagga gaagtgatag ccagagagtt gctggagtac
    14101 tgtcagtaca gagggcgtgt caggcgtctg tggttatctg cactggatga ggccagtatc
    14161 aggaaggcgc tgaataatat tctcccgggc gaacagactg cgcttctcag tgaagccggt
    14221 aaggggcgtg cccgggctga ctggctgaca gggatgaatc tgagccggtt gtataccctg
    14281 aaggcacggg aacgtggaat ttctggggtt ttgtcagtgg gccgggtgca gacacccacg
    14341 ctggcaatgg tggtcagacg ggatcaggag atcgccacat ttgtgccggt ttcctggtgg
    14401 caggtgattg tctggctgga aaaggagggg ctccgtttcc gcagtaactg ggtggcagct
    14461 gagcagtact gcgatgatga gggacgttgt gtgaatgtgc aggcggccgg tgctgttctc
    14521 ggtctgtgcc gtcagcagtc ctctgctgtt gtacaggatg ttgtacagaa gagggagaaa
    14581 acaccgccac ctttgtgttt ttcgctggga acgttgcagg aagcctgttc ccggaagttt
    14641 ggcatggggg ctcagaccgt tctggatatc gcacagtccc tttatgagac tcacaaagca
    14701 acaacttatc ccaggacaga ctgtggttat ctgcctgcct cgatgcatga ggagagttct
    14761 gaagtgcttg aggctgtcgg gcgttcagat ccgtccgtgc ttccacaact ggctgaactg
    14821 gaatcaggac gggtatcccg tatctggaat gataaaaaaa ttacggctca ccatgccatt
    14881 attccgaccc ggcaggtttt tgacctcagc tcactgaacg gtgatgaact gaaggtatat
    14941 caactgattc gtcagcacta tctggcacag tttctgccgg agcaggaagc tgatgttact
    15001 gaggcaacat ttgtgattgg ggggcagtta ttccgttccc gcggaagagt taatgtgatt
    15061 atcgggtgga aacagttatt tccaccggaa aatcatgcag atgaagaaaa gcctgacagt
    15121 gatgaaagtg gaaacatatt gccccctttg ataaagggag aactctgtgc tctggatggt
    15181 gctgaacaga aggagctgaa aaccgtacct ccccgtcctt ttactgaagg ctgtcttatt
    15241 actgcgatga agaatgcttc gtctggtgta acggatccgg cgctgaaaaa gatcctgagg
    15301 gagaatgcgg gactgggaac ggaagccaca cgcgcgggga tcctggaaac gttatttgcg
    15361 cgcaactata ttgagagagc gggaaaacat atccgttcca cgcaactggg aagagaactg
    15421 gtggcggcat tgccggactc tctgacaaat ccgggtatga cggctttatg ggaacaggcg
    15481 ctggatgatg ttgcccgggg gaaaatctca ctggaggcgt ttatggagaa acagcatcag
    15541 tggatcaggc acctggtgtt atccggacgt aaccagacgg ttaatctgtc agtggaatca
    15601 ggtccttcat gtccactctg taacggtccg acggtgaaac ggaagggaaa aagcggtttt
    15661 ttctggggat gctcccgtta tcccgactgc aggggaattg ctggtgagca gggaaacagc
    15721 aggggcagga aaagaaaaat taatttttct cataaacaaa aatctggtga tgtcggagac
    15781 aaactgaatt aaaattgcag gcgagttctg gtatcaccct gttgaccaca gtggggctga
    15841 cagccctgag aggtccggta acccgttatg cacacggcgg ggttacaagg cacatgcact
    15901 ccgtgaaaac cgtgccggga gtcgctctct ccggagaaga tgagaatcaa caccgggatt
    15961 tggtatcccc ctgaagcacc ggcttgccgg tgcttttttg tttttggtta gctgttgact
    16021 ttttccatca gtgatttcag tctgtccggg cacataacgt cgctccggcg accaccaatg
    16081 ctccacaagt ggcctgagag gagcgccttc gggtggctac
//
LOCUS       DAZUCE010000109        15975 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZUCE010000109 DAZUCE010000000
VERSION     DAZUCE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.109"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(149..1507)
                     /gene="plaP"
                     /locus_tag="ACHY03_002929"
     CDS             complement(149..1507)
                     /gene="plaP"
                     /locus_tag="ACHY03_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFS3906135.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1497..1559)
                     /gene="yoeI"
                     /locus_tag="ACHY03_002930"
     CDS             complement(1497..1559)
                     /gene="yoeI"
                     /locus_tag="ACHY03_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFS3906136.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1774..2703)
                     /gene="yeeY"
                     /locus_tag="ACHY03_002931"
     CDS             complement(1774..2703)
                     /gene="yeeY"
                     /locus_tag="ACHY03_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3906137.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2749..3573)
                     /gene="yeeZ"
                     /locus_tag="ACHY03_002932"
     CDS             complement(2749..3573)
                     /gene="yeeZ"
                     /locus_tag="ACHY03_002932"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3906138.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            3999..4049
                     /gene="hisL"
                     /locus_tag="ACHY03_002933"
     CDS             3999..4049
                     /gene="hisL"
                     /locus_tag="ACHY03_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFS3906139.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4025..4148
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4195..5094
                     /gene="hisG"
                     /locus_tag="ACHY03_002934"
     CDS             4195..5094
                     /gene="hisG"
                     /locus_tag="ACHY03_002934"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFS3906140.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5100..6404
                     /gene="hisD"
                     /locus_tag="ACHY03_002935"
     CDS             5100..6404
                     /gene="hisD"
                     /locus_tag="ACHY03_002935"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFS3906141.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTNDLAQCVAISNQYGPEHLIIQTRN
                     ARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSSLASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6401..7471
                     /gene="hisC"
                     /locus_tag="ACHY03_002936"
     CDS             6401..7471
                     /gene="hisC"
                     /locus_tag="ACHY03_002936"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFS3906142.1"
                     /translation="MSTVTITDLARENVRKLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCTQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7471..8538
                     /gene="hisB"
                     /locus_tag="ACHY03_002937"
     CDS             7471..8538
                     /gene="hisB"
                     /locus_tag="ACHY03_002937"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFS3906143.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQTDFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8538..9128
                     /gene="hisH"
                     /locus_tag="ACHY03_002938"
     CDS             8538..9128
                     /gene="hisH"
                     /locus_tag="ACHY03_002938"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFS3906144.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQSNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9128..9865
                     /gene="hisA"
                     /locus_tag="ACHY03_002939"
     CDS             9128..9865
                     /gene="hisA"
                     /locus_tag="ACHY03_002939"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFS3906145.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            9847..10623
                     /gene="hisF"
                     /locus_tag="ACHY03_002940"
     CDS             9847..10623
                     /gene="hisF"
                     /locus_tag="ACHY03_002940"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFS3906146.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10617..11228
                     /gene="hisIE"
                     /locus_tag="ACHY03_002941"
     CDS             10617..11228
                     /gene="hisIE"
                     /locus_tag="ACHY03_002941"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFS3906147.1"
                     /translation="MLTEQQRRKLDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11325..12302)
                     /gene="wzzB"
                     /locus_tag="ACHY03_002942"
     CDS             complement(11325..12302)
                     /gene="wzzB"
                     /locus_tag="ACHY03_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFS3906148.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALVETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12448..13614)
                     /gene="ugd"
                     /locus_tag="ACHY03_002943"
     CDS             complement(12448..13614)
                     /gene="ugd"
                     /locus_tag="ACHY03_002943"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFS3906149.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(13863..15269)
                     /gene="gndA"
                     /locus_tag="ACHY03_002944"
     CDS             complement(13863..15269)
                     /gene="gndA"
                     /locus_tag="ACHY03_002944"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFS3906150.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15358..>15975)
                     /gene="galE"
                     /locus_tag="ACHY03_002945"
     CDS             complement(15358..>15975)
                     /gene="galE"
                     /locus_tag="ACHY03_002945"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFS3906151.1"
                     /translation="NIPLNESSRVGNTTNPYGTSKLMVEKILQDISSSNQKFRTTILR
                     YFNPVGAHKSGEIGEDPNGTPNNLMPYICQVAIGKRKELFIYGNDYKTKDGTGIRDFI
                     HVMDLAEGHIAALKKCDNGSNYKIFNLGTGKGYSVLDLISAFERVTSVTIPYVITSRR
                     PGDIAECWSDPKKAFLELGWKAMYNLDDMVRDAWNWQKKNPSGYK"
BASE COUNT         4110 a         4081 c         3952 g         3832 t
ORIGIN      
        1 gaaattattc atgactggtg gaatgcagac caataaccaa attctctaaa ttagaacaaa
       61 tggttattaa tgaggatttc gataaaaaag aataaaaaaa ccggagccaa atgttcattc
      121 gactccggtt gattattaga aaagatgatt atgctacgtc ttcgtactgc ggaaccggat
      181 tacggaagct cttggtgacg caagccaggt aaatcagacc gatagcagcc cagatcagcc
      241 ccagaaccat tgagctttct tccaggttaa cccacagcgc accaacggtc agcgcaccac
      301 acatcggcag gaacagatac tggaagtgat ctttcagcgt cttgttacgc ttctcacgga
      361 tccagaactg cgagatgacc gacaggttaa cgaaggtgaa cgccaccagc gcaccaaagt
      421 tgatcagcgc cgtagccatt accaggtcga agttgattgc cagcagggca atcgcgccaa
      481 ccaggatgat gttcatcgcc ggagtacgcc atttcgggtg tacataaccg aagaagcttt
      541 tcgggaacac gccgtcacga cccattacgt acatcagacg cgcaacacct gcgtgcgccg
      601 ccataccaga cgccagtacg gtaatggtgg agaagatcag cgcgccaacc tggaacgctt
      661 tacccgcaac gtacagcatg atttcaggct gtgacgcatc cggatcttta aagcgagaga
      721 tatccgggaa gtacagctgc aggaagtaag ttgcaaagat gaagatcatg ccgccgatca
      781 gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc atctttggtt tcttccgaca
      841 ggttgctgat gccgtcaaag ccggtaaagg agaagcacag gattgtcgcc ccggtaatca
      901 tcgggataac atgcgcatcg ccagaccaga acggacgagt gctcgccagc gtaccagcgc
      961 cttcaccttc aaatacgcca taaacaacca tgcccagaat caccgcgatc agcactacct
     1021 gcaacacgac gattacagtg ttgaagttcg ctacggattt cagactacgc aggttaaagg
     1081 cggtcataaa ggccaccagc gccaccacaa acatccacga tgggatggaa ggcaccagag
     1141 cttcaaaata gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca
     1201 gagaagacca acccaccata aagccgacag tcgggctaat ggatttctgg gcgtaagtat
     1261 atgcagagcc agcagaagga tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca
     1321 ggatcgcaat caatgcgaac gcataggctg tcggcacatg accatccgtg aggcctgaaa
     1381 cgataccaaa agtatcaaac agcgtcatcg gctgcatata ggcaagaccc atcattacaa
     1441 ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat
     1501 gcgacatggt cattctcctt tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt
     1561 tttttggatt cctcagcgac aacaactgtc gatttttagt aaatatctat ccggtacgaa
     1621 gcccggcctc ttggtatgaa tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac
     1681 gtcggttttt agtcattttt tgacagcggc gcattgtgcc taaatggggg ggaaatgaca
     1741 agagaatgag aggcttgtca taataatttt tctttaaatg gctgattttc cgtcatcaga
     1801 ttttgcataa acaccggcga tcgcactatt tgctaaaatt tcatcccgcc accagccgct
     1861 ggcaagccct gcggtctgtt cattccagcc aatccagacg ggttcaaaga gagtttgggc
     1921 gaccactttc ttctcgatta acgcgccact atcgagaaaa cgttgcgcca gataacgggg
     1981 taaatagccg cagccgaggc cgctaatttg cagctccagc ttggttttaa aatcgaaaac
     2041 ggtaatggct tcttgttcgt cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt
     2101 gtcccccacc acaatcgccc gatatcgctt aatgatacga cggtttaacg gctcttcttc
     2161 ctgggctagc ggatgatgag gcgcgaccgc aaagacttgt tccagatcgc ccaaccgcga
     2221 gaaaccaaac tcactggaag atggtggctc atgcatcgcg ccaacaataa tatccgctcg
     2281 cccctgagtc agggcatccc aggaaccacc gagtacgcca ttgataaact tcaggcgcgt
     2341 cacgctatga tgttgataaa aagcttcaat cagtggcgca agcagagaaa aaggaaacgt
     2401 gtcgtccacg ccaatcacca gctcgttttc ccagccttca tgcagtttga tcgcctgctt
     2461 ttccagttct cgcacggtat gcagaacttc ccgccccttc tctaataaca tttttccggt
     2521 acgggtgaat ttagcgcggt gaccgctacg atcaagtaat tgaatattaa ggtcgctttc
     2581 cagcttgtga acggtgtaac tcagagcgga cggcgtcttg tagagtttgg ccgacgccgc
     2641 cgcaaaactg ccttcttttt ctaaggcatc gaggatcatc aacacatcca gcagtggttt
     2701 catacacgcc cccttgcagt gcatgcggcg tcctgctggc gtgatcgttt actccagcgg
     2761 cattaccagc ggatcgggat actggtattc aaatcccagt tcattacaaa tccgactgcc
     2821 atcaataatc ttacctttgc cgctgtccag actatttctg aactgcggag gttccagccc
     2881 cagtaaacgg gccatctgcg gatagaaaac attacgcgca gggtgagctg gcgcacatat
     2941 attatagatg tgtccgcctt taggtgcctg taacaacaga gtgatagcgc caataacatc
     3001 ttctaaatgg actaaattaa caccatgttc accatcaggc gcggtttttc cggcaaagaa
     3061 gcgtccggga tgacgtcccg gtccgaccag ccccgcaaga cgcagaatat cgaccgaagt
     3121 accgggtaaa ttgtgcagcc agtcttcgag ttcttctaat actcgtccac tgttggtcac
     3181 cggattacgc ggggtggttt ctttcaccgt gccttgcgcg tcgccataga cagatgttga
     3241 gctggtaaaa ataatacggg gaatacgatg agccagcgcg ctatccacta actcttgtac
     3301 cgcttgtaaa tagaactcat cgccggggcc gctacgacgt gccggaagcg taatgaccag
     3361 cgcgtcggca tccatcaggg catccagatc gtcagaatcg caaactaact caggctccat
     3421 gcgaagcaga tagctatcaa tgccactcat tcgggccgct tcgacaccat cttgtgtggt
     3481 tttactcccg gtgacttgcc agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa
     3541 ccaccctaac ccgacaattg cgaccttttt catccgttat ctcctgatac accttagatc
     3601 tataaggcta cgctagcgta tcaaaactga caattcattc tatgaatgaa atcgaaacgg
     3661 ttagcaaata taccattgcc tgatgcgctc cgcttatcag tcctacaaaa catcaatata
     3721 ttgaatttag gcaacatttg tagaccagat aaggcattca cgccgcatcc ggcaggaaca
     3781 aagcccactt tagttacaga acacaatgtt cttgagggtt ttaaagacat attttcacga
     3841 ttacttttct aacgtagtca ctccaggccc ccacctttcc ccgctcattc attaaacaaa
     3901 tccattgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt ggtttaggtt
     3961 aaaagacatc agttgaataa acattcacag agacttttat gacacgcgtt caatttaaac
     4021 accaccatca tcaccatcat cctgactagt ctttcaggcg atgtgtgctg gaagacattc
     4081 agatcttcca gtggtgcatg aacgcatgag aaagcccccg gaagatcacc ttccgggggc
     4141 ttttttattg cgcgactgat aacggttcag acaggtttaa agaggaataa gaaaatgaca
     4201 gacaacactc gtttacgcat agctatgcag aaatccggcc gtttaagtga tgactcacgt
     4261 gaattgctgg cgcgctgtgg cattaaaatc aatcttcaca cccagcgcct gatcgcgatg
     4321 gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg acgacattcc cggtctggta
     4381 atggatggcg tggtagacct tgggattatc ggcgaaaacg tgctggaaga agaactgctt
     4441 aaccgccgcg cccagggtga agatccgcgc tactttaccc tgcgtcgtct ggatttcggc
     4501 ggctgccgac tttcactggc aacgccggtt gatgaagcct gggatggccc actctcctta
     4561 aacggtaaac gtatcgccac ctcttatcct cacctgctca agcgttatct cgaccagaaa
     4621 ggcatctctt ttaaatcctg cttactgaac ggttctgttg aagtcgcccc gcgtgccgga
     4681 ctggcggatg cgatttgcga tctggtttcc accggtgcca cgctggaagc aaacggcctg
     4741 cgcgaagtcg aagttatcta ccgctcgaaa gcctgcctga tccagcgcga tggcgaaatg
     4801 gaagaatcta aacagcaact gatcgacaaa ctgctgaccc gtattcaggg tgttatccag
     4861 gcgcgcgaat caaaatacat catgatgcac gcaccgaccg aacgcctgga tgaagtcatc
     4921 gccctgctgc caggtgccga acgcccaacc attctgccgc tggcgggtga ccaacaacgc
     4981 gtagcgatgc acatggtcag cagcgaaacc ctgttctggg aaacgatgga aaaactgaaa
     5041 gcgctgggtg ccagttcaat cctggtcctg ccgattgaga agatgatgga gtgatcgcca
     5101 tgagctttaa cacaatcatt gactggaata gctgtactgc ggagcaacaa cgccagctgt
     5161 taatgcgccc ggcgatctcc gcttctgaaa gcattacccg cactgttaac gatattctcg
     5221 ataacgtgaa agcacgcggt gatgacgccc ttcgggaata cagcgcgaag tttgataaaa
     5281 ccacggttaa cgcgctgaag gtgtctgctg aggagattgc cgccgctagc gaacgcctga
     5341 gcgacgagct aaaacaggcg atggcggtgg cagtaaagaa tattgaaacc ttccacactg
     5401 cgcaaaaact gccgccggta gatgtagaaa cccagccagg cgtgcgttgc cagcaggtca
     5461 cgcgcccagt cgcttcagtt gggttgtata ttcctggcgg ctccgccccg ctcttctcaa
     5521 cggtattaat gctggcaact ccggcgcgta ttgcgggctg taaaaaagtg gtgctgtgct
     5581 caccgccgcc gattgccgat gagatccttt acgcggcgca gctgtgcggt gtacaggacg
     5641 tgtttaacgt cggcggcgca caggccattg ccgcactggc gtttggtacg gaatctgtgc
     5701 cgaaagtgga caaaatcttc gggccgggta acgcctttgt caccgaagcg aaacgtcagg
     5761 tgagccagcg tctggacggt gcggcgatcg atatgcccgc aggcccgtcg gaagtgctgg
     5821 tgattgctga cagcggcgct acgccggatt tcgtggcttc tgatttgctt tcccaggctg
     5881 aacacggccc ggactcacag gtgattttac tgacgcccga cgccgatatg gcgcgtcgcg
     5941 ttgccgaggc tgttgaacgc cagctggcag aactgcctag agctgaaacc gcccgtcagg
     6001 cactgagcgc cagccgcctg atcgtgacca acgatttagc gcagtgcgta gcgatttcca
     6061 accagtacgg cccggagcac ctgatcattc agacccgcaa cgcccgcgaa ctggtcgata
     6121 gcatcaccag cgccggttcg gtatttcttg gtgactggtc accggaatcc gcaggtgatt
     6181 acgcctccgg caccaaccac gttctgccga cttacggtta caccgccacc tgttccagcc
     6241 tcgggctggc ggatttccag aagcgcatga ccgtgcagga actgtcgaaa gaaggctttt
     6301 cttcgctggc ttcaaccata gaaacactgg ccgccgccga gcgcctgacc gcccacaaaa
     6361 atgccgttac tttgcgtgtt aacgccctta aggagcaagc atgagcaccg tgactattac
     6421 cgatttagcg cgtgaaaacg tccgcaagct gacgccgtat cagtcggcgc gtcgtctggg
     6481 cggtaacggc gacgtctggc tgaacgccaa cgaatatccc acagccgtgg agtttcagct
     6541 cactcagcaa acgctcaacc gctacccgga atgtcagccg aaagcggtga ttgaaaatta
     6601 cgcgcaatat gcaggcgtaa aaccggaaca ggtgctggtc agtcgtggcg cggacgaagg
     6661 tattgaactg ctgattcgcg ctttttgcga accgggtaaa gacgccatcc tctactgccc
     6721 gccaacgtac ggcatgtaca gcgtcagcgc cgaaaccatt ggcgtcgagt gccgcacagt
     6781 gccgacgctg gacaactggc aactggactt acagggcatt tccgacaagc tggacggcgt
     6841 aaaagtggtc tatgtttgca gccccaacaa cccgaccggg caactgatca atccgcagga
     6901 ttttcgcacc ctgctggagt taacgcgcgg taaggcgatt gtggtcgccg atgaagccta
     6961 tatagagttt tgtacacagg catcgctggc tggctggctg gcggaatatc cgcacctggc
     7021 tattttgcgc acactgtcga aagcttttgc tctggcgggc cttcgttgcg ggtttacgct
     7081 ggcaaacgaa gaagtcatca acctactgat gaaagtgatc gccccctacc cgctctcgac
     7141 gccggtcgcc gacattgcgg cccaggcgtt aagcccgcag gggatcgtcg ccatgcgcga
     7201 acgggtggcg caaattattg cagaacgcga atacctgatt gccgccctga aagagatccc
     7261 ctgcgtggag caggtttttg actctgaaac caactacatt ctggcgcgct ttaaagcctc
     7321 cagtgcagtg tttaaatctt tgtgggatca gggcattatc ttacgtgatc agaataaaca
     7381 accctcttta agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa gccagcgcgt
     7441 cattgacgcc ttacgtgcgg agcaagtttg atgagtcaga agtatctttt tatcgatcgc
     7501 gatggaaccc tgattagcga accgccgagt gattttcagg tggatcgttt tgacaaactc
     7561 gcctttgaac cgggcgtgat cccggaactg ctgaagctgc aaaaagcggg ctacaaactg
     7621 gtgatgatca ctaatcagga tggtcttgga acacaaagtt tcccgcaaac ggatttcgat
     7681 ggcccgcaca acctgatgat gcagatcttc acctcgcaag gcgtgcagtt tgatgaagtg
     7741 ctgatttgtc cgcacctgcc cgccgatgag tgcgactgcc gtaagccgaa agtgaaactg
     7801 gtggaaggtt acctcgctga gcaagcgatg gatcgtgcca acagttatgt gattggcgat
     7861 cgcgcgaccg atattcaact cgctgaaaac atgggcatta atggtttacg ctatgaccgc
     7921 gaaaccctga actggccaat gattggcgag cagctcacta aacgagaccg ttacgcccat
     7981 gtagtgcgca acaccaaaga gacgcaaatt gacgtccagg tgtggctgga tcgtgaaggt
     8041 ggcagcaaga ttaataccgg cgttggcttc tttgatcaca tgctggatca gatcgccacc
     8101 cacggcggtt tccgtatgga aatcaacgtc aaaggcgacc tctatatcga cgatcaccac
     8161 accgtcgaag ataccggcct ggcgctgggc gaagcgttaa aaattgccct cggcgataaa
     8221 cgcggtattt gtcgctttgg ttttgtgcta ccgatggacg aatgccttgc ccgctgtgca
     8281 ctggatatct ctggtcgccc gcacctggaa tataaggccg aatttaccta ccagcgcgtg
     8341 ggcgatctca gcaccgaaat gatcgagcac ttcttccgtt cgctctcata caccatgggc
     8401 gtgaccctgc acctgaaaac caaaggtaaa aacgatcacc accgtgtaga gagcctgttc
     8461 aaagcttttg gtcgcaccct gcgccaggcc atccgcgtgg aaggcgatac cctgccctcg
     8521 tcgaaaggag tgctgtaatg aacgtggtga tccttgatac tggctgcgcc aacctgaact
     8581 cggtgaagtc tgccattgcg cgccacggtt atgaacccaa agtcagccgt gacccggacg
     8641 tcgtgttgct ggccgataaa ctatttttac ccggcgttgg cactgcacaa gcagcgatgg
     8701 atcaggtgcg tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa ccggtgctgg
     8761 gcatctgctt agggatgcaa ctgcttgggc ggcgcagcga agagagcaac ggcgtcgact
     8821 tgctgggcat cattgacgaa gacgtgccga aaatgaccga ctttggtctg ccgctgccgc
     8881 atatgggctg gaaccgcgtt tacccgcagg caggcaaccg cctgtttcag gggattgaag
     8941 atggcgcgta cttttacttt gttcacagct acgcaatgcc agtcaatccg tggaccatcg
     9001 cccagagtaa ttacggcgaa ccgttcactg cggcggtaca aaaagataac ttctacggcg
     9061 tgcagttcca cccggagcgt tctggtgccg ctggcgctaa gttgctgaaa aacttcctgg
     9121 agatgtgatg attattccag cattagattt aattgacggc accgtggtgc gtctccatca
     9181 gggcgattac ggcaaacaac gcgattacgg taacgacccg ctgccacgct tacaggatta
     9241 cgctgcgcaa ggtgccgaag tgttgcatct ggtggatctg accggggcaa aagatccggc
     9301 taaacgtcaa atcccgctga ttaaaaccct ggtcgcgggc gttaacgttc cggtgcaggt
     9361 tggcggcggc gtgcgtagcg aagaagacgt ggcggcgtta ctggaagcgg gcgttgcgcg
     9421 cgtagtggtc ggctccaccg cggtgaaatc accagatatg gtgaaaggct ggtttgaacg
     9481 cttcggtgcc gatgccttag tgctggcgct ggatgtccgt attgacgagc aaggcaacaa
     9541 gcaggtggca gtcagcggct ggcaagagaa ctcgggcgtt tcactggaac aactggtgga
     9601 aacctatttg cccgtcggcc tgaaacatgt gctgtgtacc gatatctcgc gcgacggcac
     9661 gctggcaggc tctaacgtct ctttatatga agaagtgtgc gccagatatc cgcaggtggc
     9721 atttcagtca tccggcggca ttggcgacat taacgatgtt gctgccctgc gtggcactgg
     9781 cgtgcgcggc gtaatagttg gtcgtgcatt actggaaggt aaattcaccg tgaaggaggc
     9841 catcgcatgc tggcaaaacg cataattcca tgtctcgacg ttcgtgatgg tcaggtggta
     9901 aaaggcgtac agtttcgcaa ccatgaaatc attggcgata tcgtgccgct ggcaaaacgc
     9961 tacgccgaag aaggtgcaga cgaactggtg ttctacgata tcaccgcttc cagcgatggc
    10021 cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag tgatcgacat tccgttctgc
    10081 gtggcgggtg ggattaagtc tctggaagat gccgcgaaaa ttctttcctt tggcgcggat
    10141 aaaatttcca taaactcgcc tgcgctggcg gacccgacgt taattactcg cctggcggat
    10201 cgatttggcg tgcagtgtat tgtggtcggt attgatacct ggtacgacgc tgaaaccggt
    10261 aaatatcatg tgaatcaata taccggtgat gaaagccgca cccgcgtcac tcagtgggaa
    10321 acgctcgact gggtacagga agtgcaaaaa cgcggtgccg gagaaatcgt cctcaacatg
    10381 atgaatcagg acggcgtgcg taacggttac gacctcgaac aactgaaaaa agtgcgtgaa
    10441 gtttgccacg tcccgctgat tgcttccggt ggcgcgggca ccatggaaca cttcctcgaa
    10501 gccttccgcg atgccgacgt tgacggcgcg ctggcggctt ccgtattcca caaacaaata
    10561 atcaatattg gtgaattaaa agcgtacctg gcaacacagg gcgtggagat caggatatgt
    10621 taacagaaca acaacgtcgc aaactggact gggaaaaaac cgacggactg atgccggtga
    10681 ttgtgcaaca cgcggtatcc ggcgaagtgt taatgctggg ctatatgaac ccggaagcct
    10741 tagataaaac catcgaaagc ggcaaagtca ccttcttctc gcgcactaaa cagcgactgt
    10801 ggaccaaagg cgaaacgtcg ggcaatttcc tcaacgtagt aaatattgcg ccggactgcg
    10861 acaacgacac gttactggtg ctggcgaatc ccatcggccc aacctgccac aaaggcacca
    10921 gcagctgctt cggcgacacc gctcaccagt ggctgttcct ttatcaactg gaacaactgc
    10981 tcgccgaacg caaatctgcc gatccggaaa cctcctacac cgccaaactg tatgccagcg
    11041 gcaccaaacg cattgcgcag aaagtaggtg aagaaggcgt ggaaaccgca ctggcagcga
    11101 cggtacatga ccgctttgag ctgaccaacg aggcatctga tttgatgtat cacctgctgg
    11161 tgttgttgca ggatcaggat ctggatttaa cgacggtgat tgagaacctg cgtaaacggc
    11221 atcagtgagt tgcggggtaa gcggatgcga tattgttgtc gcatccggca aaaaaaacgg
    11281 gcaaggtgtc acccccctgc cctttttctt taaaaccgaa aagattactt cgcgttgtaa
    11341 ttacgcagag cgttacgccc caagacaatc cccgcgccaa ccatgccgcc cagcagcacc
    11401 gccagaatca aggtaattgc ctttttcggg ctatcgcgac gaataggtaa cgtcggtttc
    11461 atcacataac ggtaagcatg aatatcaaga tcatcaacct ttaagttttc aatatccaac
    11521 aggttttgac gtgtctgata gtagtttggt gagaacacca acggacgggt cgcctcatgc
    11581 ttaatcatcg actccagcgc ttcgctaccc aaaaggaaca acgtatcttg cgtcacatct
    11641 tcagtctgct gaacctgtgg ctttgccacc tgcgcctgat tcgcatactg caacgcttcc
    11701 tgaatctgac ggatacgcag atctttctgc tcctgcgcga ctacttcctg cgttcttaaa
    11761 gagtcctgca agtttttccg tcccagagca atgttgtctt tgagatcctt ttctagctct
    11821 tgattcactt tatcatcaac ttgctgaatg tattgggcca acttcatttg tgctccctct
    11881 gcagtttgcc caacataaga aacagtcaat ggtaattgct ggttcttaac agaaggttcg
    11941 atggtaagtt tttctggttc ttcctgatta tccagcgttt ctactaatgc agagaaggca
    12001 gaactgaagc gaccaatcaa cgtctcctgc aaatccgata ctttcggtgc agcctgacca
    12061 tagataacat tcatggcatt gttatagcca gcaatttgcc ccacgtcggg ctgagtgata
    12121 attgctgttg acgtccattt ctccttcgct actgccaaat aaccaatagc cagagcaata
    12181 gccacaatga cggaaatgat gattgtcatc ttgccacgcc acaactgcac tagtaaatca
    12241 atcaaatcaa tctgttccgg gtcatggttt tgcccagaaa cattattatt ttctactctc
    12301 atcattaccc taactgacgg aaatttctaa gtccgcatag tttagcgata atccccgtga
    12361 atgctatagg ataagtgata aaaataatga attattgcgg aggtaacaaa agacaatcag
    12421 ggcgtaaata gccctgataa caagatgtta gtcgctgcca aagagatcgc gggtgtagac
    12481 cttatctgcc acatccctaa gctcttctgc catacggttg gaaataatga cgtcggcttg
    12541 ttgtttgaag gtggcgagat cacgttccag gcgagagttg aagaatgagt cttctttcat
    12601 cactggctcg tagatgatca cttcaacacc tttcgccttg atacgtttca taatcccctg
    12661 aatagaagac gcacggaagt tatctgaacc gctcttcata atcagacgat aaatacctac
    12721 cacttgcggc ttgcgtgaca aaatggcatc ggcaataaaa tctttacgcg tgcggttagc
    12781 atcgacaatt gccgagatca ggttattcgg cacagactgg tagttcgcca gtaactgctt
    12841 ggtatctttc ggcagacaat aaccaccata accaaacgac ggattgttgt aatggttgcc
    12901 aatacgcgga tcgagacaaa cgccttcgat tatttgacgg gaattcagac ctaaactttc
    12961 tgcgtagcta tccagttcgt taaagtacgc cacgcgcatc gccaggtagg tgtttgcaaa
    13021 aagtttaatc gcttctgctt cagtggagtc ggtaaacagg gtcgggatat tttgcttaat
    13081 cgctccttcc tgtaacaatg cagcaaaacg ttctgcgcgt tctgaacgct caccaatgac
    13141 aatacgtgaa ggatggagat tatcgtaaag ggctttacct tcacggagaa attccggaga
    13201 gaaaataata ttttcagtac gatatttctt atgcatcgct tcggtaaaac caacgggaac
    13261 cgttgatttg atgaccataa ccgcataagg atttatctca actacgtctt taattactga
    13321 ttctacactg gatgtattga aataattagt tttaggatca tagtcggttg gagtggcgat
    13381 gatgacataa tcagcatccc ggtaggcttc atttttatct aatgtggcat taaagtgtat
    13441 tttatctgat tgcaaaaact gctgaatttc cttatccaca ataggagata tccgatcatt
    13501 cagcatagca acgcgtgacg gtaaaatatc taatgccaca acctcatgat tttgtgcgat
    13561 tagaagcccg tttgacaagc ctacatagcc agtaccggaa atggtaattt tcatttcatg
    13621 ctctcagaat taacttaact gtgaatcatg atgtttttag catcctgata agagttaaaa
    13681 gttttaacgc tacgttgttc gatggtcaac gcaacgaaaa acaattcaga ttttctttat
    13741 aagaatagct gatatttatt aagttaatat taagcagtga aatttagtct ataagatatt
    13801 tggcaaaaaa aagcccggtg caatatacgc cgggcctcaa ttttattgtt ggttaaatca
    13861 gattaatcca gccattcagt atggaacaca ccttctttat caatgcgctt ataagtatgc
    13921 gcaccgaaat agtcacgctg tgcctggatc aggtttgcag gcagaactgc ggcgcggtag
    13981 ctgtcataat aggcaaccgc agccgcgaag gtcggaaccg ggataccgtt ctgtactgca
    14041 taagcaacga catcacgcag cgcctgctgg tagtcatcgg caatttgctt gaagtacgga
    14101 gccagcagca ggttagcgat ctgcggattt tcggcataag catcggtgat tttctgcagg
    14161 aactgcgcac ggatgatgca gccagcacgg aaaatcttcg cgatttcgcc gtagttcaga
    14221 tcccagttgt actcttcaga cgcagcacgc agctgagaga agccctgggc gtaagaaacg
    14281 attttgccca gatacagcgc acgacgaact ttttcgatga actcagcctt gtcgcctgct
    14341 ggctgtgctt gcggaccaga gagaacttta gatgcggcaa cacgctgatc tttcagagaa
    14401 gagatataac gtgcaaacac agactcggta atcagcgaca gcggttcgcc gagatccagc
    14461 gcgctctggc tggtccattt accggtacct ttgttagccg cttcatccag gatcacatca
    14521 accaggtagt taccgtcttc atcttttttg gtgaagatat ctttggtgat gtcgatcagg
    14581 taactgctca gttcaccgtt attccactcg gtaaaggtct gcgccagttc ttcgttggtg
    14641 aggttcaggc cacctttaag cagagaatag gcttcagcaa tcagttgcat atcaccgtat
    14701 tcgataccgt tgtgaaccat cttcacataa tggcctgcgc catcggcacc aatataggta
    14761 acgcatggct ccccgtcttc agctactgcg gcgattttgg tcaggattgg tgcaaccagt
    14821 tcataggctt ctttctgccc accaggcata atagaaggac ctttcagcgc gccttcttca
    14881 ccaccggaga caccggtacc aatgaagtta aagccttcgg cagaaagttc acggttacga
    14941 cgaatggtgt cctggaagaa ggtattgcca ccatcaatga tgatgtcacc tttatcgaga
    15001 tatggtttga gggaatcaat agcagcatcc gtgcctgcac ctgctttcac cattaacagg
    15061 atgcgacgag gcgtttccag agattcaaca aactctttca ccgtatagta aggaaccagt
    15121 ttcttgcctg gattttcggc aatcacttct tccgtctttt cacgggaacg gttgaaaata
    15181 gagacggtat aaccacggct ttcgatgttg agcgcaaggt tgcgccccat cactgccata
    15241 ccgactacgc cgatctgttg ctttgacatt gtttactcct gtcaggggat tttcacatac
    15301 aatacataaa cattgctgag attaatataa atccgttatg aaccgataat agtattttta
    15361 tttataacca ctaggatttt ttttctgcca attccatgca tctcttacca tatcgtccaa
    15421 gttatacatc gccttccaac ctaattctaa gaaggctttt ttggggtctg accaacactc
    15481 agcaatgtca cctggacgtc ttgatgttat gacatatggt atggtaacag atgtaacacg
    15541 ctcaaaagct gaaataagat cgagtacgga atatcctttt cctgtaccta aattaaaaat
    15601 tttatagttg cttccattat cacatttttt caaagcagca atatgcccct ctgcaagatc
    15661 cattacatgt atgaaatcac gaatccctgt cccatctttt gttttataat cattaccata
    15721 aataaataat tcctttcgct taccaatggc gacttgacat atgtaaggca tcaaattatt
    15781 tggtgtacca ttaggatctt ccccaatctc accggatttg tgagctccca ctggattaaa
    15841 atatcttaat atagtcgtac gaaatttttg gttagagctt gagatatctt gtagtatttt
    15901 ttcaaccatt aacttcgatg ttccataagg atttgttgta ttaccgactc gcgaagattc
    15961 gtttagcggt atatt
//
LOCUS       DAZUCE010000110        15901 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZUCE010000110 DAZUCE010000000
VERSION     DAZUCE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.110"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..690
                     /locus_tag="ACHY03_002946"
     CDS             <1..690
                     /locus_tag="ACHY03_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFS3906152.1"
                     /translation="YLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSWGINNR
                     DDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVAQTAMC
                     CGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYVRAHHYYHSWMHYFSAYS
                     LGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPLTVQPE
                     CPVTLKDVSDL"
     gene            687..2138
                     /gene="djlC"
                     /locus_tag="ACHY03_002947"
     CDS             687..2138
                     /gene="djlC"
                     /locus_tag="ACHY03_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFS3906153.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRALLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMDGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQMEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSAQHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHNGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSIISIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2198..3868)
                     /gene="hscC"
                     /locus_tag="ACHY03_002948"
     CDS             complement(2198..3868)
                     /gene="hscC"
                     /locus_tag="ACHY03_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFS3906154.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIEIP"
     gene            complement(3952..4887)
                     /gene="rihA"
                     /locus_tag="ACHY03_002949"
     CDS             complement(3952..4887)
                     /gene="rihA"
                     /locus_tag="ACHY03_002949"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFS3906155.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5005..5730)
                     /gene="gltL"
                     /locus_tag="ACHY03_002950"
     CDS             complement(5005..5730)
                     /gene="gltL"
                     /locus_tag="ACHY03_002950"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFS3906156.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5730..6404)
                     /gene="gltK"
                     /locus_tag="ACHY03_002951"
     CDS             complement(5730..6404)
                     /gene="gltK"
                     /locus_tag="ACHY03_002951"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFS3906157.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6404..7144)
                     /gene="gltJ"
                     /locus_tag="ACHY03_002952"
     CDS             complement(6404..7144)
                     /gene="gltJ"
                     /locus_tag="ACHY03_002952"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFS3906158.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7314..8222)
                     /gene="gltI"
                     /locus_tag="ACHY03_002953"
     CDS             complement(7314..8222)
                     /gene="gltI"
                     /locus_tag="ACHY03_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFS3906159.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKNKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            complement(8619..10157)
                     /gene="lnt"
                     /locus_tag="ACHY03_002954"
     CDS             complement(8619..10157)
                     /gene="lnt"
                     /locus_tag="ACHY03_002954"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFS3906160.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10182..11060)
                     /gene="corC"
                     /locus_tag="ACHY03_002955"
     CDS             complement(10182..11060)
                     /gene="corC"
                     /locus_tag="ACHY03_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFS3906161.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11150..11617)
                     /gene="ybeY"
                     /locus_tag="ACHY03_002956"
     CDS             complement(11150..11617)
                     /gene="ybeY"
                     /locus_tag="ACHY03_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFS3906162.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11614..12654)
                     /locus_tag="ACHY03_002957"
     CDS             complement(11614..12654)
                     /locus_tag="ACHY03_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFS3906163.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(12807..14231)
                     /gene="miaB"
                     /locus_tag="ACHY03_002958"
     CDS             complement(12807..14231)
                     /gene="miaB"
                     /locus_tag="ACHY03_002958"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFS3906164.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14377..15552
                     /gene="ubiF"
                     /locus_tag="ACHY03_002959"
     CDS             14377..15552
                     /gene="ubiF"
                     /locus_tag="ACHY03_002959"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFS3906165.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGVALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(15706..15780)
                     /locus_tag="ACHY03_002960"
     tRNA            complement(15706..15780)
                     /locus_tag="ACHY03_002960"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15746..15748),aa:Gln,seq:ctg)
BASE COUNT         3829 a         4058 c         4147 g         3867 t
ORIGIN      
        1 tatcttcttt ttgcgctttc tgcgcccatg gaaatactta atcagggttg caaacccgcg
       61 catgactcgc caaaaatgta taccgggata aaagaattcg atctctcatc atcctgggga
      121 ataaataatc gtgatgattt aattcagact atttatcaga tgactgatga tggtcatgct
      181 aatgatttag ctggcttata tctgacatgg catcgctctt cccctgaaga gtggaaagca
      241 ttaattgctg gtggctcgga gagagggttg atttataccc aattcgttgc ccaaaccgct
      301 atgtgctgtg gagaaggggg aatcaaagct tgggattatg ttcgaatggg gtttctgtca
      361 cgggtcggtg tgctcaacaa gtggctgacg gaagacgaga gtttatggct gcaatcgcgc
      421 gtctatgtac gagctcacca ttactatcac agctggatgc actatttttc tgcctattca
      481 ctagggcgtc tctactggca atcttctcag tgcgaagaca acacttcgct acgcgaggcg
      541 ctaaccctgt acaaatacga tagcgccggg agtcgtatgt tcgaagaact ggcagcggga
      601 agcgatcgtt tctatgccac gcttccctgg cagccattaa ctgtccagcc tgaatgcccg
      661 gtaacgctta aggatgtgag cgacctatga aaacatgttg gcaaatcctc gaaattgaaa
      721 gcacgacgca aatagacatt atccgccagg cttatcttgc tcgcttaccg ttatgtcatc
      781 ccgaaaccga tccgcagggg tttaaagcat tacgccaggc ctatgaagag gcgctgcgac
      841 tggcggtaaa tcctgtcgag gaagcagatg atgaagaaaa agatgccgcc gctgaacatg
      901 aaatactacg tgcattcagg gcattactgg attcagaaag tgatcgtttt cagccctccg
      961 cctggcagaa atttattcag caattaaata cctggaacat ggaggatgtc gatcaattac
     1021 gctggccgct gtgtgcaatc gctatagaag cgcgatatct ttcattgaat tgtgcttctt
     1081 tgctggcaga gcgtttgaac tggcattcgt ttaatgacag cgaaggaatg gatgaggaag
     1141 aaagggaggc ttttcttgag gccattcagg ctggtgattg tttcgatttc cttagccttc
     1201 tggaatatcc cgttgcgttg cagaaccaga cggttgagta ttacttcgcg ctggaacgat
     1261 gctgccgtta ccatcctgac tatgtcactg cgtttttggc gatggatggt ccgtggttca
     1321 ttcctgatga tgcgaagttg catcgcaaac tgttgcgctg gtacagctcg gtgcaaacag
     1381 gtatggcgga actcattcct gtcgctcaac agtggcaaat ggaagaacca gaaagcgaag
     1441 atgcccggta ttaccagtgt gcacaacgtt tgtactgcgg cgagggggaa agcctgcttg
     1501 ccgatctctg cgcgtactgg gaaagttacc catctacaca agccgataat ttgttgttgc
     1561 agtggagcgc acaacattgc ccggattatt tcgcgttatt agtgatggtt atcgaagcgc
     1621 ggagcatggt agatgcgcaa ggtaaaccgt tgaaatatgt tcctggtgag agcgcccgga
     1681 cgcgtctgtt atgggcggaa attttacata acggaaaact atcgccgtta ggtcaatcgt
     1741 ttattgagtc gttattcttc aaacgcaaag catgggcgtg gtggaaatcg agagtcggta
     1801 gcgagacaga agaggattca ccgttactgg atttgtatcg ggtagcggaa caggtagtac
     1861 ttgaagcgtt tccgaaacaa gagatgctgg cccgtctcaa tacaaggctg gaaggcggag
     1921 atgctcatcc attagaggcc attatcaccc ggatgctgtt gacgaaagta aaactcgagc
     1981 cggaggatga agatgtcgat gagccaacac ctgaaaacca tgaagaaaaa aatgatgagg
     2041 atgaaaaacc acagagcatt atcagcatta tcaaaatcag cttaacggtg ctggtgatag
     2101 gttatgctct cggcaaaatc gcgatgttgt ttagttgacg atgtgttttt tgcgccgatg
     2161 ccggatgcag catttacgcc gcatccggca gccgtgatta tgggatctca atggctaaat
     2221 aaacgctcgc ccgccgcctg acctcatcga cgcgggccat atcgtttgac tgcagcgccg
     2281 catcaaaatc ggtgatcacc cggccaatct cttctcgctc gtcacccagc gcccgcgccc
     2341 acaactcttc cagtttggct ttaaaggtgc ggttgatgag catatcgcgc ggataaattt
     2401 tcaatgcgga taaccgcgtc cgactctctt caatctgctg cgcgctcaat gttaccgggc
     2461 tgtggttaat cactctggac ttaacgctgc cgtcttccag aagcacgtca acttcaagca
     2521 agccgttgat atcataacta aagcgaatat caatcgactg ataagccccg gttttcttca
     2581 acggcacatc gaaggattcc accagaatgt tatttttaac tttgtggttt tctccctgat
     2641 agacgttaac cgtaatagaa tcctgttccg ggtgcatggt cgagtaagtt tctacacgcg
     2701 acaccggtac tgtggtgttt cgttcaataa tcggcgagaa aatgccggaa acgccctggc
     2761 ggttaacttc aacgcccaac gagtaagggc aaatatcagt gaggatcact tcttcaatat
     2821 cttcactgcg taagcggcag gctgcctgga ttgctgcgcc cagcgcgaca atggtgctcg
     2881 gatcgtaact ttgatacggt aatttgccaa acagacgcac ggcgattcgc tgcaccagcg
     2941 gcatttgtga cgcgccgcca accagcacca gactgtcgat ttgactcggc ttcagacgcg
     3001 catcgcgcaa cgcctgttca atcggcactc gcaagcgatt gagcagcggc aaccacaaat
     3061 cttccagttc gttctcgtaa aattcgcatt cccgcgtttc atcctggtac tgccagcgaa
     3121 tgtgcattgg cgattgattg ctacattttg ccgcttccac acaggcatac aaggctgcca
     3181 gttcactctc gttaagcgtg gtcctggcga catccgcgcg ttttaaaacc tcatcgacca
     3241 gcatatgggt aaaatcttcg ccaccgagga agttgtcgcc agcggaggcg tgaacttcaa
     3301 tcaccggcgt ggcgtactca agcaccgtaa cgtcaaacgt gccgccaccg agatcaaaca
     3361 ccagcgaacg ggtattttgt tgggtatgca ggccatacgc catcgcagct gctgtgggtt
     3421 cattaattaa gcgtaccgca tttaacccgg ctaactccgc tgctaaacgg gtatgcttgc
     3481 gttgttcatc actgaaataa gccggaacag agatcaccac atctttaatc ggacgttgca
     3541 gaaattcttc ggcatcttct tttaatgagc gtaataccaa agaggacagt tctggcgcgt
     3601 taaatgtgtc gctgcctaac cgccagttgg tattactgcc cattgcacgt ttaaataacg
     3661 ctgccgtttt atccggatgc gaagtacgcc gtgatacagc cggttttccg actaaaatat
     3721 gattattttc atccatgcta attatggatg gtgttaaata ttcaccgaac ttatttggaa
     3781 ttaattgcgc ggcaccgtct ttccagacgg caattaaact attggtagta ccgagatcga
     3841 taccaatggc gagttctgca ttatccattg catttatatc ttgaaagaaa aagagataaa
     3901 cagattaaga ccacccaggt aacgcgtggt gatcttaatc aatgacgtgt gttaagcgta
     3961 aaatttcaga cgatcagcca gtaaatcaac aaagccctga cgatcaacat cgaccattac
     4021 agtggcattc ggtttattgc cagtcagata ataataatca acaaccgtca tcccctgggt
     4081 atatttcccc tgtgtttcca cgccaaccca gcgctcaaca gtggtaaaca actcgggttt
     4141 caacagccag gcgatggtgc atgggtcatg cagcggtgcg ccgacaaagc cccatttttc
     4201 gtctttatga tattcgagga agaaatccag cagttcggca acaatggttg aaaccgggtt
     4261 accaatcgcg cggaaacgct cggtgtcctc aacgtggatt tgcgctttgt gagtaacatc
     4321 cagaccggcc atcaccaccg ggatccctga ctggaagaca atttctgccg cttccgggtc
     4381 aacgtaaata ttaaattcag ccgcaggcgt ccagttacca agccccattg cgccgcccat
     4441 aatcacgata cgggcaattt tgctatgcag ttccggatgg ctattgagca gcaaggcaac
     4501 gttagtttgc ggtccggtag aaacaatggt gacaggttcc gcactttcac gcagcgtttt
     4561 cgccattagc tctaccgccg tacagttttg cggtgcgaac gccggttccg gtaatgccgg
     4621 gccgtcgaga ccgctttcgc cgtgcacatt gtccgcgata atcaactcac gcattaacgg
     4681 ttttaccgcg ccgcctgcta ccggaatatc ggtgcgatta agcaaggtca gcatacgcag
     4741 aacattgcgt aaggtttttt ctggtgtctg gtttccggcg gaagacgtaa ttgctttgac
     4801 atcaagctct ggtgaggcga gggcgagaac tattgcgata gcgtcgtcat gacctgggtc
     4861 gcaatctaac agaattggca gtgccattgt tgctccttgt tgtgtgcttc tttgcgacaa
     4921 gggtaacgcc aggatgtaac agatacgagg ggcgaaacga taaagcgtga gatggcgcgc
     4981 aattgggtat gcgcgccaga gtgattaatg caggattttc gcgaggaagt cttttgcgcg
     5041 gtccgatttc ggatcatcga agaacgcatc tttcggcgag tcttcgacaa ttttaccctc
     5101 atccataaag atcacccgat tcgccacttt acgggcaaag cccatttcgt gggtcaccac
     5161 catcatggtc atcccctcgt tcgccagttc gaccatcacg tccagcactt cgttgatcat
     5221 ctccgggtct agtgccgatg tgggttcgtc gaacagcatc gcaataggat ccatacacaa
     5281 tgcgcgagca attgccacac gctgctgctg gccgccggaa agctgcgccg gaaatttatt
     5341 ggcatgtgcg gaaagtccca cgcgctccag caatttcaat gctttttcac gagccggtgc
     5401 tttatcgcgt ttaagcactt tcacctgcgc cagggtcagg ttttcgataa tcgacagatg
     5461 agggaacagc tcgaaatgtt ggaataccat cccgacgcga gaacgcagct ttgccagatc
     5521 ggttttcttg tcgttaacca cgataccatc gacggtgatt tcaccttgct gcactggttc
     5581 gaggccgttg acggttttaa tcagcgttga tttgccggaa ccagacgggc cgcaaaccac
     5641 caccacttcg ccttttttca cttcggttga gcagtcggtc agcacctgaa agtgaccata
     5701 ccattttgaa acatttttca gggtaatcat tatgctgtcc ttcttttcaa gtagctgacc
     5761 aacaacgacg cgctaaggct aataacgaaa taaacaaatc cggcaaacag gatcatctca
     5821 acctgcgtac catcacgctc accaatggtt gaggcggtac ggaagaaatc ggccagagat
     5881 aacacataca ccagcgaggt atcctggaac agtacgatgc cctgagtgag cagcagcggc
     5941 accatcgcgc ggaacgcctg cggcagaata atcagtttca tcgactgcca gtgagtcatt
     6001 cccaacgcca gcgcggcgct agactgacca cgagaaatac tttgaatacc agcacggata
     6061 atctctgaat agtaggccgc ttcaaacatc gaaaacgcca ccatcgccga aattaaacgg
     6121 atatcatttt ttggtgataa tcccagcacg ttttgcagaa aacccggcac gatcaggtaa
     6181 aaccacagca aaaccataac taaaggaatc gagcggaata cgttaacgta ggctttggca
     6241 aaccacgcta cgggcgcaaa gctggacaag cgcatcaccg ccagcatggt gccccacaaa
     6301 ataccaatca cgactgccgt gacggtgatt ttcagggtga tcaccagccc gtcgagcaga
     6361 tatggcaggg aaggaacaat ggaactccag tcaaactcgt acattatttg ccccccatgt
     6421 tgccgggcag gcgaacttta cgttcaacca gcgtcatcac cagcatgata aaagcgttaa
     6481 tcaatacata cgccagcgta atggcggtaa acgactccca ggcgtgagcc gagtagtcga
     6541 gcaatttacc cgcctgcgct gccatatcta ccagaccgat ggtcgaggcg atggcggagt
     6601 ttttcaccag gttcatcatc tctgaggtca tcggcgggac gataacgcga taagcattag
     6661 gcagcagtac gtagcggtaa gcctgcggta gcgtcagacc catcgccagc gcggcatttt
     6721 tttgcccgcg cggcagcgac tgaatcgcgg cgcgtacctg ttcgcaaaca cgggcggcgg
     6781 taaacagccc caggcagagc atggatgaaa ggaaaaactg aatattggga tccagctcgg
     6841 ccttaaacca catgccgatt ttctccggca gcagctccgg gatcaccaga taccaggtaa
     6901 agaattgcac aatcaacggc acgttgcgga acagttcgac atacaacgta cccagaccgg
     6961 agagaaaacg gtttggaacg gtacgtaaaa tgccgaaaaa tgaaccgacg aggaaagcga
     7021 taatccaggc gcagatcgac aaagcgatcg tcacctgaaa accactccag atccaaccaa
     7081 gataggtggt gttgccgaac ggggcttgtt gtaaaaaaat accccagttc cagtctatag
     7141 acataaatct actccagaaa aaagagggta gcagcgttaa ctgctaccct cgaagattgt
     7201 tacccagcgt attgcggttt tcaggcccga tggggaacga ccatcaggcg tatagtctgt
     7261 ccgtgctacg taacaatcga gagggctgga atttccgccc ctggttcttg taattagttc
     7321 agtgccttgt cattcggttc tttgaacagt gctttcattt cgtctgacag ttcgaaattc
     7381 atgttcaggt ttttcggcgg aattggattt ttgaaccatt tatcaaacca tttttccgct
     7441 tcaccggagg tctgcacctg agcgatagtg tcatccatca gctttttgaa ctgcggatcg
     7501 tctttacgca acatgcaacc ataggcttcc tgagactgcg gcttgccgac gatatcccag
     7561 ttgtctggtt tcttcgcttt cgcacgttca ccggccagca gagcatcatc catcataaag
     7621 gcaacggcac gaccgctttc cagggtacgg aaagagtcac cgtgatcttt ggcgctgatg
     7681 atgcgcatat tcattttttg ctcttcattc agtttgttga gcaaaacttc agaggtagtg
     7741 ccggaagtga cgactacggc tttgcctttc aggtcggcaa aatctttgat atcgccgccc
     7801 tttttggtca acaggcgcgt accgaccacg aaaatagtgt cagagaaagc cgcctgtttt
     7861 tggcgttcga cgttgttggt ggtagaacca cattcaaaat cgaaagtgcc gttttgcaga
     7921 agaggaatac ggttttgtga ggtgatcgga attagtttca cctgcaagtc cggtttgttg
     7981 agtttgtttt tcactgcttc aacaatggcg ttggagtaat cctgcgaata acccaccact
     8041 ttttgctgat tgtcgtaata agagaaaggt actgaagatt cacggtgacc gacgacaatc
     8101 acgccgtttt tggcgatttt gtccagcgta ctgcccgctg ccggggcggc gtcatctgcc
     8161 tgtgccagtc ctgcggaaag cgcaagggcc aggattgctg tggcaggttt acgtaattgc
     8221 atatccaact cctttatctt ctgcgttaag aacgcatgga tacccgttgt gagtgtttgt
     8281 gttgttatcg tctgcaactt tattgtgcag tgttgtgact gttaggtaac atttagtttg
     8341 gctaaatgta aagatattga tgttttattg tttgtttttg cgaggtgcac cgcaccattc
     8401 cgaagcaaaa ttcttaaaat gcactctttt ggtgctaccg gtggattgct gtggtgcaac
     8461 cgggttgtac tgatgctggt taaagctttg ccttgtataa caaagcaata gatgtgccaa
     8521 agttggatag gagaatattg ttatccggat aacgcactga tgccgcatcc ggtgagcgtg
     8581 ccgaaatatg ggatgtattc cggcacgata agaagggatt atttacgtcg ctgacgcaga
     8641 ctcatcaaca cagcagcaaa accaaacagt gccgtcagca cccacagcgg ccagttgccg
     8701 gtacgtgcgt atggtgtgag tccggtggtc ggcgtcacgt tagtggttaa cacctcgcgg
     8761 gtgaactgcg ggatcatcgc ctgaatctca ccctgcgggc caatcaccgc cgtaatgccg
     8821 ttgttggtgc tgcgcaacag tgggcgcgcc agctccagcg cacgcattcg cgccatctgg
     8881 aagtgctgcc atggaccaat agacttacca aaccacgcat cgttggagat agtcagcaga
     8941 tagtcggtat ccgggcggaa gttatcgcgc acttgctcgc cgagaatgat ctcgtagcaa
     9001 atagccgcag taagctcaat accatttgcc gacagcggcg gctggatata tggtccacgg
     9061 ctgaacgacg acatcggcag atcaaagaac ggtgctaacg gacgcagaat cgactccagc
     9121 gggacaaact cgccaaacgg caccagatgg tttttgttat agcgatcggc tgattcgtag
     9181 ctgtacggtg cacctttacc cagtgtgatg atggtgttgt aggtatcgta acggttctgc
     9241 ttattaagac gcgcgtcaac aatcccggtg accagcgagc tacctttatc acgcaactca
     9301 ccgtccagag ctttgaggaa cggttgctga ttaatttcca gatcggttat cgccgactcc
     9361 ggccagataa tcaacgatga tttgcccatc agcggtgccg ttgcgttgta gtaaatcttc
     9421 agcgtattaa gaagctggcc ttcgtcccat ttcagcgatt gcggaatatc gccctgaacc
     9481 atcgaaacct gaatggtttt ctccggttgt ggggtaaacc actggatgta acgcagcggg
     9541 aagggaaggg caaacagcac gacggccacc accagcggac gccagttgcg tttgactaac
     9601 gccagtgcca gcaggccgct aaccatcata agcaggaagt taatggcttc cacgcccatt
     9661 agcggtgcca gcccttttaa cgggccatca atctggctat agccgaactg taaccacggg
     9721 aagccagtca gcacccaacc gcgcagaaac tcggtcactt gccagagagc aggagcggca
     9781 atcgccacgc gcagccaggt ggttttcggc cacagacgcg atagcacgcc agcaaacagt
     9841 ccggtataca gcgacaaata cgccgccagc agtaccacca ggaagatgtt aaccgggcca
     9901 ggcattccgc caaaggtcgc gatgctgaca tagacccagt taataccgct gccaaagaga
     9961 ccaaatcccc agcaaaagcc aatagcggca gactggagtg gacggcggtt aaaggtcaac
    10021 gcctgaagcc ccatcagcga aataatcgcc gcaggccaga cgtcgtaagg agagaaggcc
    10081 agcgttccgc aggcaccgaa taataacgcc agcagcaggc gaatgcgctg gcgttcaatt
    10141 aatgaggcaa aagccatgta gttatctatc cagtttcggt tttattcatc cagcttcggc
    10201 tggggtgagt catccggaat tttgacatga acctgaataa tacgccgact gtcagccatc
    10261 gccactttga actggtaacc gtcgatgtcg atggtttcgc cacgcgccgg aagatgcccg
    10321 aatgcctgca tcaccagacc accgatagtg tcgacctctt catcgctaaa gtgggtgccg
    10381 aacgcttcgt tgaagtcttc aatggaagcc agtgcgcgca cggtccaggt atgacgactc
    10441 agctgacgga agtcgatatc atcttcttcg tcatattcgt cttcaatctc accaacaatc
    10501 agttccagga tgtcttcaat ggttaccaga ccggaaaccc caccgaattc gtcaataacg
    10561 atcgccatgt ggtaacgctg agagcgaaac tctttcagca tccggtctac gcgcttactt
    10621 tcaggaacga caaccgcctg acgtaacact ttgtccatgc tgaaggcttc agcatcgctg
    10681 cgcataaacg gcagcaagtc tttcgccatc agaatccctt caatgtgatc tttgtcttcg
    10741 ctaatcaccg ggaaacgtga gtgggcggac tcgatgatga catcaaggca ttcgtccagc
    10801 gtctggttgc gtttcagggt aatcatctgg gagcggggga tcatgatgtc gcggacgcgt
    10861 tggtctgcga tgtccatcac cccttcgagc atatcgcgcg tatcttcgtc gataaggtcg
    10921 ttctgcccgg aatcacggat cagcgccagc agttcgtcac ggtttttcgg ttcaccgtgg
    10981 aaaagttggc tgagtaacag ggagaaaaat cccttcttgt tgcttatcgt gtcactactg
    11041 tgtgaattgt cgtcgctcat ggcgtcgtat gggttctcat gttagttaat caaaacgccg
    11101 tcgttaatca ccaacggcgg ggacgtctgc cagtcaaatg cctggcaaat tattctttct
    11161 cggcaatgta cggatcctca tagcccagag caagcataat ctctgtttcg agggcttcca
    11221 tttcttctgc ttcgtcatct tcgatgtgat cgtaacctaa caaatgcaga ctgccgtgca
    11281 ccaccatatg cgcccagtgc gcctccagtg gtttgccttg ctcctgagct tccttctcaa
    11341 ccacctgacg gcagataacc agatcgccca gtagcgacat ctccatgccg ggcggcactt
    11401 caaacgggaa ggagagcacg ttggtcggct tatccttacc gcgataggtc agattgagac
    11461 tgtggctttc ggcggtatcg accacgcgaa tcgtcacttc cgactcttcc tgaaactgcg
    11521 ggatcaccgc attcagccat gtttgaaact ggctctcttc cggcaatccg gagttatctt
    11581 cacatgccag ttgtaaatcg aggatcacct gactcatttt tgttcctgtt cttcgcgctt
    11641 gcgttctgcc gccagtgccg ctttacgttt ttgttcggct tcttcccagg cttcataggc
    11701 gttaacgata cgcgccacca ccgggtgacg aaccacgtct tcgctgtgga agaagttaaa
    11761 gctgatctct tcgacatcgg ccagcacttc gatggcatga cgtaagcctg atttagtatt
    11821 acgcggcagg tcgatctgtg tgacgtcgcc ggtgataacc gcttttgagt taaaaccgat
    11881 acgggtcagg aacatcttca tctgttcgat ggtagtgttc tggctctcat cgagaatgat
    11941 aaatgcgtcg ttcagcgtac gaccacgcat ataggccagc ggtgcgactt caataacgtt
    12001 gcgctcaatc agtttctcga ctttctcaaa gcccagcatt tcaaacagcg cgtcgtacag
    12061 tgggcgcaga tacgggtcta ctttctggct taaatcgcca ggcaggaagc ccagtttctc
    12121 accggcttcg accgccggac gagtcagcag aatacggcga atttcctgac gctccagggc
    12181 atcaactgcc gcagccactg ccaggtaggt tttacccgta cccgccgggc caacgccgaa
    12241 ggtaatgtca tggtcgagaa tattggcgat gtactgcgcc tggtttggcg tgcgcggctt
    12301 aattacgccg cgtttggttt tgatattgac cgctttgccg tactccggca cgctctccgc
    12361 gctttgctcc agtacccgcg cttctttaat cgcaaggtgg atctgttccg gttcaatatc
    12421 ctgaatctga ccgcgcatcg gggcagtatc gacatacagg ctacgcagaa tgtctgccgc
    12481 agcggtgacg caaatcggac ggccggtcag tttaaagtgg ttatcgcggc gattgatctc
    12541 gatgccgaga cggcgttcga gctgcttgat gttgtcatca aacgggccgc acaggctcaa
    12601 cagacgcgcg ttgtctgctg gctccagggt gatttcgcga gtgtctatgt tcaaaccgtt
    12661 cctcttatct gtatgccgcc ggaagctgaa cattcaccgg cctataagga aattattcac
    12721 gccacaggaa aaaggcgcaa gcattgcgat aaagatgggg ataaagagag aaaaaacaag
    12781 gcccaccgga acggcgggcc tgagaattac ggctgataat aacccacgcc aaggtcgttt
    12841 tctttgcgag tacgggctat caccgattcc ggtgtttctg ccacgcgcag acccatttca
    12901 tcttcagttc gcaccacttt accgcgcaga gagttcgggt agacgtcggt aatttctaca
    12961 tcgacgaatt taccgatcat atccggcgtg ccttcgaagt tgaccacgcg gttattttcc
    13021 gtacgcccgg aaagctccat gatgctctta cgcgatgtac cttctaccag aatacgctgg
    13081 gtggtgccga gcatccggcg gctccacgcc atcgcttgct gattaatgcg ctcttgcaga
    13141 atatacagac gctgcttctt ctcttcttcc ggaacatcat caaccatatc ggcggctggt
    13201 gtacccggac gtgcagagaa gataaagctg tagctcatgt cgaaattgac gtcggcaatc
    13261 agcttcatcg ttttctcgaa gtcttcggtg gtttcgccag ggaagccgac gatgaagtca
    13321 gaactgatct gaatatccgg gcgcgccgca cgcagtttac ggatgattgc tttgtactcc
    13381 agcgccgtat gggtacggcc catcaggttc agaatgcgat cggaaccact ctgtaccggc
    13441 aggtgcagga agctcaccag ctccggcgtg tcgcggtaca cttcgatgat atcgtcggtg
    13501 aattcgatcg gatgactggt ggtaaagcga atacgatcga tcccgtcgat cgctgcaacc
    13561 agacgcagca gatcggcaaa cgatccggtg gtgccgtcgt agttttcacc acgccaggcg
    13621 ttcacgttct gaccgagcag gttgacttca cgcacgccct gagccgcaag ctgggcaatc
    13681 tcaaacagaa tatcgtcgga cggacggctt acctcttcac cacgggtgta aggcaccacg
    13741 cagtaggtgc aatatttatt gcagccttcc atgatggaga caaacgcggt cggcccttcg
    13801 gcgcgcggtt ccggtagacg gtcaaacttc tcgatttccg ggaagctgat atctacaacc
    13861 gggctgcggt cgccgcgcac ggagttgatc atctccggca gacggtgcag cgtttgcggc
    13921 ccaaaaataa tatcgacata gtgggcgcgc tggcgaatgt gctcgccttc ttgcgatgcc
    13981 acgcagccac cgacgccgat aatcaggtct ggattcttct cttttaacag tttccagcga
    14041 cccaactgat ggaagacttt ttcctgagcc ttctcgcgga ttgagcaggt gttcagcagc
    14101 agcacatccg cttcttccgc cacgtcggtc agttgatagc cgtgggtggc atccagcaga
    14161 tcggccatct tcgatgaatc gtactcgttc atctgacagc cccaggtttt aatatggagt
    14221 tttttggtca tcgacttgct cttgcgaaat agtagccagg aatgcagggc gcatagtgta
    14281 atgctttgct gccgttgtga ccagtatgag cgttatcagc ccttgggggt aaaaatcctg
    14341 taaacttaaa gcatattgct aacaggatga ttgaccatga caaatcaacc aacggaaatt
    14401 gccattgtcg gcggaggaat ggtcggcggc gcactggcgc tggggctggc acagcacgga
    14461 tttgcggtaa cggtgatcga gcacgcagaa ccagcgccgt ttgtcgctga tagccaaccg
    14521 gacgtgcgga tctcggcgat cagcgcggct tcggtatcat tgcttaaagg gttaggggtc
    14581 tgggatgcag tacaggccat gcgttgccat ccttaccgca gactggaaac gtgggagtgg
    14641 gaaacggcgc atgtggtgtt tgacgccgct gaacttaagc taccgctgct tggctatatg
    14701 gtggaaaaca ctgtcctgca acaggcgttg tggcaggcgc tggaagcgca tccgaaagta
    14761 acgttacgtg tgccaggctc gctgattgcg ctgcatcgcc atgatgatct tcaggagctg
    14821 gagctgaaag gcggtgaagt gattcgcgcg aagctggtga ttggtgccga cggcgcaaat
    14881 tcgcaggtgc ggcagatggc gggaattggc gttcatgcat ggcagtatgc gcagtcgtgc
    14941 atgttgatta gcgtccagtg cgagaacgat cccggcgata gcacctggca gcaatttact
    15001 ccggacggac cgcgtgcgtt tctgccgttg tttgataact gggcatcgct ggtgtggtat
    15061 gactctccgg cgcgtattcg ccagttgcag aatatgaata tggcacagct ccaggcggaa
    15121 atcgcgaagc atttcccgtc gcgtctgggt tacgttacac cgcttgccgc tggtgcgttt
    15181 ccgctgacgc gtcgccatgc gttgcagtat gtgcagccag gggttgcgct ggtgggcgat
    15241 gccgcgcata ccatccatcc gctggcgggg cagggggtga atcttggtta tcgtgatgtc
    15301 gatgccctga ttgatgttct ggtgaatgcc cgcagctacg gcgaagcgtg ggccagttat
    15361 cctgtcctca agcgttacca gatgcggcgc atggcggata acttcattat gcagagcggt
    15421 atggatctgt tttatgccgg attcagtaat aatctgccgc cgctgcgttt tgtgcgtaat
    15481 cttggtttga tggcggcgga gcgtgctggt gtgttgaaac gtcaggcgct gaaatatgcg
    15541 ttagggttgt agccttacaa cattgccgga tgcgtgccaa ccgtaggtcg gataagacgc
    15601 ggcagcgtcg catccgacat tgaaggataa gacgtgtcaa catcgcattc gacattgaat
    15661 gaacgcagaa aagcaaaaag ctcgccgaag cgagcttttt taatgtggct ggggtacgag
    15721 gattcgaacc tcggaatgcc ggaatcagaa tccggtgcct taccgcttgg cgatacccca
    15781 acaaattggt tttgaatttg ccgaacatat tcgacacatt cagaatttgg tggctacgac
    15841 gggattcgaa cctgtgaccc catcattatg agtgatgtgc tctaaccaac tgagctacgt
    15901 a
//
LOCUS       DAZUCE010000111        15588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZUCE010000111 DAZUCE010000000
VERSION     DAZUCE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.111"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..569
                     /locus_tag="ACHY03_002961"
     CDS             <1..569
                     /locus_tag="ACHY03_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906166.1"
                     /translation="WEGIVSFGEACVKFGKIIWAVAKKLEWLIPDEKTDEEQRVYNEE
                     YNKAYQEFMDSGGKYSPNPGLAADNVAKAKADEAVDNMRHPERLARAKSQAESMLSFA
                     NPFTGLINKDSTPESQNSATLNIDALRQAVATPAPEQNNKIEINIIGAADPQSTQQAA
                     ASGVLDGLRQVASSYNRGAMFDKPAAAG"
     gene            572..994
                     /locus_tag="ACHY03_002962"
     CDS             572..994
                     /locus_tag="ACHY03_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3906167.1"
                     /translation="MSDAINGAEDIMLGLGDFIFAISTVAYNKLQRSDAWRWAQQTRF
                     GKNDALQITGRPNPTITIDGKINALFLDGCGVGLLTDLRALGNTGEPQQLVLGTGEVK
                     GYWVLRELTETQNSFLMGGTPKSQDFSLTLEYYGASLD"
     gene            1168..1380
                     /locus_tag="ACHY03_002963"
     CDS             1168..1380
                     /locus_tag="ACHY03_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFS3906168.1"
                     /translation="MITYTTRDGDRLDQICLAVYGRTAKTTETVLYQVLNYGVTDMCA
                     VFRAGEKIVLPEIDPEPVKKETQLWD"
     gene            1384..2427
                     /locus_tag="ACHY03_002964"
     CDS             1384..2427
                     /locus_tag="ACHY03_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late control protein"
                     /protein_id="HFS3906169.1"
                     /translation="MSDYVNTGVEAWKPNFYISADNENITDKIRKGLINITLTDYGGS
                     SKQTDELRVAIVSETLKIPARGVKISVGLGFGNQIIDKGIYIVDGASSGGEPRVVDFT
                     AKAAPMNASKGSPTVQSKKTRSWSDVTVSNIVATIASDNGLKPRVSKRFADNIITQLD
                     QVGESDMHLMSRLATRFDAVSKPAGGYWIFLPRGAGESVSGAPLQHYTLTRRDNSSWG
                     YSRNGQSGDSGSGGENPEPTYLIKYHDTATGQIKELRTGSGGDPVIEWPAVEPSLDAA
                     KEAAPGLKGGAAKKEFSMTYTTPATLDLVSLTAECKVTTQGFGTEEDRDWTINTLSLT
                     LAENGFSVRLSLE"
     gene            2594..3013
                     /locus_tag="ACHY03_002965"
     CDS             2594..3013
                     /locus_tag="ACHY03_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906170.1"
                     /translation="MVVKNALACGIMLTLVGCATKPVSNEQATPVPEKQIISTVLLEK
                     RDGTGEVIIKRDSGFFGSACFSRVYVDGKEVADLGTAQKVVVYPTIGDHVFSAWPKGV
                     CGGGMSEQAGKVTTDRTLMYRIGYGSNGEYGIHPTAF"
     gene            3486..4151
                     /gene="yecA"
                     /locus_tag="ACHY03_002966"
     CDS             3486..4151
                     /gene="yecA"
                     /locus_tag="ACHY03_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFS3906171.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(4213..5424)
                     /gene="tyrP"
                     /locus_tag="ACHY03_002967"
     CDS             complement(4213..5424)
                     /gene="tyrP"
                     /locus_tag="ACHY03_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFS3906172.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            5616..5855
                     /gene="yecH"
                     /locus_tag="ACHY03_002968"
     CDS             5616..5855
                     /gene="yecH"
                     /locus_tag="ACHY03_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFS3906173.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            complement(5892..6389)
                     /gene="ftnA"
                     /locus_tag="ACHY03_002969"
     CDS             complement(5892..6389)
                     /gene="ftnA"
                     /locus_tag="ACHY03_002969"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFS3906174.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6560..6883)
                     /gene="yecR"
                     /locus_tag="ACHY03_002970"
     CDS             complement(6560..6883)
                     /gene="yecR"
                     /locus_tag="ACHY03_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFS3906175.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLAYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7146..7232
                     /gene="azuC"
                     /locus_tag="ACHY03_002971"
     CDS             7146..7232
                     /gene="azuC"
                     /locus_tag="ACHY03_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFS3906176.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7347..7598
                     /gene="yecJ"
                     /locus_tag="ACHY03_002972"
     CDS             7347..7598
                     /gene="yecJ"
                     /locus_tag="ACHY03_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFS3906177.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(7677..8180)
                     /gene="ftnB"
                     /locus_tag="ACHY03_002973"
     CDS             complement(7677..8180)
                     /gene="ftnB"
                     /locus_tag="ACHY03_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFS3906178.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            8977..9966
                     /gene="araF"
                     /locus_tag="ACHY03_002974"
     CDS             8977..9966
                     /gene="araF"
                     /locus_tag="ACHY03_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFS3906179.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            10036..11550
                     /gene="araG"
                     /locus_tag="ACHY03_002975"
     CDS             10036..11550
                     /gene="araG"
                     /locus_tag="ACHY03_002975"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFS3906180.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            11565..12551
                     /gene="araH"
                     /locus_tag="ACHY03_002976"
     CDS             11565..12551
                     /gene="araH"
                     /locus_tag="ACHY03_002976"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFS3906181.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            12718..13518
                     /gene="otsB"
                     /locus_tag="ACHY03_002977"
     CDS             12718..13518
                     /gene="otsB"
                     /locus_tag="ACHY03_002977"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFS3906182.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            13493..14917
                     /gene="otsA"
                     /locus_tag="ACHY03_002978"
     CDS             13493..14917
                     /gene="otsA"
                     /locus_tag="ACHY03_002978"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFS3906183.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(14924..15352)
                     /gene="uspC"
                     /locus_tag="ACHY03_002979"
     CDS             complement(14924..15352)
                     /gene="uspC"
                     /locus_tag="ACHY03_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFS3906184.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         4255 a         3523 c         3922 g         3888 t
ORIGIN      
        1 tgtgggaggg gatagtcagt tttggcgagg cgtgcgttaa attcggaaaa ataatttggg
       61 cagtagcaaa aaaacttgag tggttaatac cggacgagaa aacagacgag gaacagcgcg
      121 tatataacga agaatataat aaagcgtatc aggaattcat ggatagtggt ggtaaatatt
      181 caccgaaccc aggactggca gcagataatg tagcaaaagc aaaggccgac gaagccgtag
      241 ataatatgcg acaccctgaa cgtcttgctc gtgcaaaatc acaggctgaa tcaatgctct
      301 cgtttgctaa cccgtttact ggattaataa ataaagactc aacgcctgaa tcacaaaact
      361 cagcaacctt gaatattgac gcgctgagac aggcagtcgc aactccagcg ccagagcaaa
      421 acaataaaat tgaaatcaat attatcggag cggcagatcc acaatcaaca cagcaggctg
      481 ctgcatcggg tgtgctcgac gggttgagac aagttgcgag ttcgtataat cgtggcgcga
      541 tgtttgataa accagcggcg gcggggtgac aatgagtgat gcaataaacg gtgctgagga
      601 tatcatgctc ggcctgggtg atttcatttt tgctatatca acagtagcgt acaacaaact
      661 gcaacgtagt gacgcctggc gctgggctca gcaaacacga ttcgggaaaa acgacgcgtt
      721 gcagataacc ggacgaccga accccacaat cactatcgat ggaaagataa acgctttatt
      781 tcttgacggg tgcggcgtcg gcctgttgac agatttgcgt gcgcttggga acacgggaga
      841 accccagcag ctagtgctgg ggaccgggga ggtgaaaggg tactgggtgc tgcgagaact
      901 aactgaaacg caaaacagct ttttgatggg cggaacccca aaatcgcagg acttttcact
      961 cacgctcgaa tattatggag cttcgctaga ttaatataaa atcaagcgct accagagcac
     1021 ttaacaagta taaaaataat aaaaaaaact acgaacataa atgtaaaaca acctgatggt
     1081 attttttttg acgccgaaat actaatacct ctccgactac taaaattaat tctcattatg
     1141 aaatcctttt aattaatggg gccccaaatg attacataca ctacacggga cggtgaccgt
     1201 ctcgatcaga tctgccttgc tgtctacggg agaacagcaa aaacgacaga aaccgtgtta
     1261 tatcaggttt taaattacgg cgtaacggat atgtgtgccg tgtttcgtgc aggagaaaaa
     1321 attgttttac cagaaatcga ccccgaaccg gttaaaaaag aaacgcaatt atgggattga
     1381 tgaatgagtg attacgtaaa tactggcgtg gaagcatgga agcccaattt ttatatatct
     1441 gctgataatg aaaacatcac ggataaaata agaaaaggtt tgatcaatat cacgctcaca
     1501 gattatggcg gttcgagtaa acagacagac gaattacgcg tagcaatcgt ttctgaaact
     1561 ctgaaaattc cggcccgtgg cgtgaagatc agtgtcggac tcggtttcgg taatcagata
     1621 attgataagg gtatatacat cgtcgacggt gcgagcagtg gcggtgaacc tcgggtagtt
     1681 gatttcacag caaaagcggc tccgatgaac gcgtcaaaag gctctccgac agttcagagc
     1741 aaaaaaactc gctcctggag cgacgtcact gtcagcaata tcgttgcaac aatagcgagc
     1801 gataacgggc taaaaccacg tgtatcaaag cggtttgcag acaatattat cacgcaactc
     1861 gatcaagtcg gcgagtcaga tatgcacctg atgtcgagac tagcaacacg attcgatgcg
     1921 gtgagtaaac ccgcgggagg gtactggata ttcctgccgc gcggcgcggg cgaatctgtt
     1981 agcggagcgc cgcttcagca ctacacgctc acgcgtcgtg ataactccag ctggggttat
     2041 tcacgaaacg gacaaagtgg cgatagtggt agcggtggcg aaaatccgga accgacgtat
     2101 ctgattaaat atcacgacac agcaactggg cagatcaaag aattgcggac aggtagtggc
     2161 ggcgatcctg tcattgaatg gccggcagtt gagccgtcgc tcgacgcagc gaaagaagct
     2221 gcaccagggc tcaagggcgg tgcagctaaa aaagagttct ctatgacgta cacgacgcca
     2281 gcgacgctcg atttagtgtc gctgacagca gaatgcaaag tcacaactca aggattcggg
     2341 accgaggagg accgggactg gactatcaac acgctgagcc tgacgcttgc tgaaaacggc
     2401 ttttcggttc gactgtctct ggagtaaatt ggctcggcga gccggtggcg ggtatgaatt
     2461 tagtattaac gactgtcgac tgcaaacggc acctgcgata tttttaggcg taagccggag
     2521 aacgaagagt ctctggtgct gaacgttgtt atcgttagat gctgacattt tttaatattg
     2581 ggagttttca aacatggtcg ttaaaaatgc tcttgcctgt gggattatgc ttactcttgt
     2641 cggttgtgct acaaaacctg tctcaaatga acaagccact cctgttcctg aaaaacaaat
     2701 tatcagtacc gtgttactcg aaaaacgaga cggtacggga gaggtgatca tcaagcgtga
     2761 ctcggggttc tttggaagcg cgtgcttttc ccgtgtttat gttgatggta aagaggtggc
     2821 agatttaggt acagctcaga aagtcgttgt atacccaacg attggtgatc atgtcttcag
     2881 cgcgtggcct aaaggggtat gtggcggtgg tatgagtgag caggcgggta aggtgacaac
     2941 agatcgtact ttgatgtatc gcatagggta tggatcaaac ggtgaatatg gcattcaccc
     3001 aactgcgttt taatttgtgt tggggtatgt gagtttttct catacccctt tacctcactt
     3061 tgctcctgac tgtttggtca catcaaggcg caaaacatca ccattttttg ccaaaaacct
     3121 tcagaagatg atgagaaaga cgtgtcataa ctatatgatt atttgatatt cacacacacc
     3181 atcatgtccg gataatttgc cataattatt tgaatttatt gcacaaaacg aacaatttaa
     3241 aatccctcgg cgttcgcgct gtgcgggttc aagtcccgct ccgggtacca tgggaaagat
     3301 aagaataaaa tcaaagcaat aagcagtgtc gtgaaaccac cttcgggtgg tttttttgta
     3361 cctgcaactt gtcgttacac cctccttaat ttttaatcac cggcaaagcc actcaaaaag
     3421 tatctctgat acggaccggc atgtaagata ggtgctggcg agttgagatc cacaaggaaa
     3481 agcgtatgaa aacgggaccg ttaaacgaaa gtgagttgga atggctggac gatattctga
     3541 ccaaatacaa cactgaccac gccatccttg atgtggcgga gctggacggt ttattgacgg
     3601 cggtgttgag ttctccgcaa gagattgaac cggcacagtg gctggttgcc gtgtggggtg
     3661 gggctgacta tgtgccgcgc tgggcgtcag agaaagagat gacgcgcttt atgaacctgg
     3721 cttttcaaca tatggccgat accgcagagc gtctgaacga attcccggag cagtttgagc
     3781 cgttattcgg cttgcgagaa gttgatggca gcgagctgac gattgttgag gagtggtgct
     3841 ttggctatat gcggggcgtg gcactttctg actggtcaac attgcccgat tcgttaaaac
     3901 cagcgctgga ggcgattgcg ctgcacggta ctgaggaaaa cttcgagcgg gtagagaaga
     3961 tgtcgccaga agcgtttgaa gagagcgtag atgccattcg actggcggcg cttgatctac
     4021 atgcgtactg gatggcgcat ccgcaggaaa aagctgttca gcaaccgatc aaagcagaag
     4081 agaaaccggg gcgtaacgat ccttgcccgt gcggtagtgg taagaaattt aagcagtgct
     4141 gcctgcatta agaagtcaaa ttgggtgtca gcgttaattc tggcacccaa taaggaattt
     4201 gaggctatct gatcacccca cttctggtaa caaccctgcc gcaatcaaaa attgcacgcc
     4261 aatcacagca ataccacaga gaaacaccac caccagcgcc ggacgaccac ctttgacccg
     4321 gtaacccgct tgaggattgt gctttctgct ttgccaggtc aacagcgaag ggataatcaa
     4381 tgccagtacc gccagcgcca caccggcgta acccagcgcc atcacaaatc ctcgtggata
     4441 aaacagtgca aacgccaacg gcggcagaaa ggtaattgca ccagtttgca atcttccacc
     4501 aacggtattt gaacgctgaa acaaatcagc cagataatca aataagccta acgcaacgcc
     4561 gagaaatgac gtggcgaggg ctaaatcagc aaataaatgc actgccagct caacatgcgg
     4621 agaggccacc atttcgcgta acgcctgtag cagcccgttt aatccggcat gattagccag
     4681 caatcccata aaggttgttg aatcaatgct gccaagcgtc gccacctgcc agaaaatata
     4741 tgccaccagg gggatcgcac taccggttat aaacacccag cgtagcttac gaatgttgcc
     4801 atccatatag ctgacaatac tcggcacgct accgtgaaaa ccaaacgacg taaaaatcac
     4861 cgggattgca gacagtgcca gcccctgttg caacggcagg gttaaaagat tcactttgtg
     4921 aatatgcggt agcagcaata ccagcatcac caccagaaaa ataattttgg cgctgaacag
     4981 aaaacggtta aataaatcga ccagcgatgt tccaacacaa accacgccac cggcaacaaa
     5041 agtgaacaac agcacgccag cggttgccga cattgaaata cctgtccagt cgctgatgct
     5101 ggaggccaac aattcaccgg caccgctgat gtatgccgct gtcagagcat acattaagaa
     5161 catcatactg aagcccgtca gccattgacc gtagcgtccc agatagcgtt ttgccagcgt
     5221 gcccagaccg gtatctgccg gaacatgctg gtacacctcc agcagtaata gcgccgtgta
     5281 gcacatcaac gcccaaagcc caatcaacaa gattaacgta acgctaaaac caacaccggc
     5341 cgcagccagc ggcattgcca gcatgcctgc gccaattgtg gttcccgcca cgataaaaac
     5401 acttcccagg gttctgtttt tcacgctttc ttctgtcctg acgatcttta tgagtgatat
     5461 ctgcggcgca ggttaaggca taacggctgc ttcgtcaaac cgacgttaca tatggtgtaa
     5521 atataaatgt acaataaaaa gcattcagtc ctgcgctggc gcaaagtctt tataagcggt
     5581 aatcgctacg ctaggctttt ttttgaggag gaacgatgga ctctattcac ggtcatgaag
     5641 tgttaaatat gatgattgaa tcaggcgagc aatatacgca tgccagtctg gaagctgcga
     5701 ttaaagcgcg ttttggtgaa caggcacgtt ttcacacttg ctcggcagaa gggatgacag
     5761 cgggagagct ggtagcgttt ctggcagcaa aaggcaaatt tataccttca gaagaaggtt
     5821 tttcgaccga tcagagtaag atttgccgtc actgatttga aacggcggca gaaacctgcc
     5881 gccggagagc attagttttg tgtgtcgagg gtagagagtt ctttgtcgat aaaatacaga
     5941 ccttcgccgc ttttgcctgc caggcttaat ttatcaataa tcgatttgaa cagtttctct
     6001 tcttcatgct gctcagaaac ataccactgc aggaaattaa atgttgggta gtcctgattg
     6061 gtcattgcag catgagccag ttcgttaatt ttctgggtga ttaattgttc gtgtttatag
     6121 gtttcctgga ataattcatc aagtgaggaa tattcagcaa acggagattc aacggtatta
     6181 atacgcggta aattgccggt atcagtcagg taatcaaaca gacgctgcat atgcgtcatc
     6241 tcttcctggg cgtgacggcg caggaacgcg gcagcacctt cgaaggtatg atagctgcac
     6301 caggcgctca tttgctgata aagcagtgaa gagtacagtt ccaggttcat ctgctcatta
     6361 agtttttcaa tcatttctgg tttcagcatg atagtgctcc acaaaggtta tatttgttgc
     6421 tgtggcgtca ctataatttg ttattttatt atttgcaaaa ggtaaaatat aaacttcaat
     6481 tattaaaaat acgaatggga ataaaacgca ttaccgctat ttctttaatc agcgaagatc
     6541 ccctcgctga ttaaaatatt tagagaaaga cagggaaggc ggaatgatgg cactgatatg
     6601 acaaagtgaa ttccgtattc aaacataagg aacccgcaaa aaggctacag ttgcctactg
     6661 gctgaccaaa ggggatcgcg tgggtatagc cttcctgctg gcatgcgcga tcggcgatcc
     6721 cgcgactgac ataacgatcg gtatgggcat gctgcaaaaa agcctggtca taagccaacc
     6781 gtacaatacc tgttgccgcg tcaacttcgc tcacatgggc ctggcgagta accgtacacc
     6841 ccgccagcgt tattaatgaa agagtcagaa tcagaagtct catggtccag ccagaagagt
     6901 aaaagaaagc actattttac gcgttacagc gctgcgacaa tggccggaaa agacgcctgg
     6961 caggcgctat ttcttgccat ctgtaggtgt cagaaatggg gtagctgctt tttgattatc
     7021 atttttataa cattaaaggg gtatttatgt gatttgtatc acataaagat tgatttttgt
     7081 gatggttgtc atattataag cgcaaagaca ataacacctg tataacaaat ggtcggagtg
     7141 ccgcgatgaa actgcgcaaa atcctgaaaa gtatgttcaa taactattgc aagacgttca
     7201 aagacgtacc gccaggcaat atgttccgat aacaaaaaac ctgctccggc aggttttttt
     7261 gtgtcctgat gacggtggat gtgactatcg ctgaagatgt gaagccgcta ctataacggc
     7321 tttcaaaatg ctatcaggag tttacgatgt cccagccgct gaatgccgat caggaactgg
     7381 tttctgatgt ggttgcttgc cagttggtta tcaaacaaat tctggatgtt ctcgatgtaa
     7441 tcgcgcccgt tgaagttcgc gaaaaaatgt ccagccagct gaagaatatc gactttacta
     7501 accatcccgc cgccgcagac ccggtcacca tgcgggcaat ccaaaaagcc atcgcgctga
     7561 ttgaactcaa gtttacccca caaggcgaat cccattaaaa aaacgctgct tcaaaaagat
     7621 gaggcagcgg tttctcaaaa acttcatcgg cgcaatgcat tagcgccgat gtatgatcaa
     7681 tgcagctgat gcgacacgac attcagaaca tgttggtcgg tctgcactgg gcacatacca
     7741 gcaagtttcg cactgcgcac ttcatcaaga atggtttgca gcaacaggcc atcatgctgc
     7801 tgttcttttt ccagatcgcg caggaaattg acggttgaat catcattcag ttctttcgct
     7861 tcatcggcta actgtgccaa cgtactagaa cgttgctcgt attcttccat cgttttttgg
     7921 aacagttctt ccagagagtt cagtttttcg ccgggaacat caatggcttt aacgatgggg
     7981 gtagcgccga cactcttcat aaagttaaac atgcgcatca tttgggtcac attactctgt
     8041 gcctgggcgc gaaggaaagt ggcggtgccg ttcagactct gttcagaaca ccagttactc
     8101 aggtgaaggt agagattgga tgcgtaaaac tcgcggttca tttgagagtt gagtttgaga
     8161 agcattccag cggttgccat acctaatatc cttatatcca gaagtggaga ggtgcaagat
     8221 aaatcaaaac accataatta ctgtttgcag aataattcct ttctgccggt ccgaaatgtg
     8281 atcgtggagt caattctgac gatgatctga aataagaata acgtctgaac gagtataaga
     8341 aaatgcttaa atcatcaagt ttatatattt ttacatttca ttgatgaaat caatgtaact
     8401 gcaatgaatt ttaatgatag tgctttttgt atcttgttga tattaatcga tgaatttgaa
     8461 ttcatgatga agtgatgatt ttataaaacg ttttttcatt tttgcgagat ggctctcatt
     8521 atacgtgttc tgttaattaa tgagcacagt gataattaat ggcgaaatga atgacgcgca
     8581 ttttccacat ctttgagttg tggttattac accatttcaa aaactcaacg ccaggtaatg
     8641 cggactattg actggttaaa aagaagacat cccgcatggg taccaaagac aacaaggatt
     8701 tccaggctaa tcttatggat taatctgctg tgcattcgac aatttgtctg acaaattggc
     8761 tttcccttat gtcttttccc gctaaattta tgcacgttct cactgtaatt ctgcgatgtg
     8821 atattgctct cctatggaga attaatttct cgctaaaact atgtcaacac agtcaattat
     8881 cttttagtta aaaggtaatg ctttgttttc cgattaattt aacgaatgtc attcgttttt
     8941 gccctacaca aaacgacact aaagctggag agaaccatgc acaaatttac taaagccctg
     9001 gcagccattg gtctggcagc cgttatgtca caatccgcta tggcggagaa cctgaagctc
     9061 ggtttcctgg tgaagcaacc ggaagagccg tggttccaga ccgaatggaa gtttgccgat
     9121 aaagccggga aggatttagg gtttgaggtt attaagattg ccgtgccgga tggcgaaaaa
     9181 acattgaacg cgatcgacag cctggcagcc agtggcgcaa aaggtttcgt tatttgtact
     9241 ccggacccca aactcggctc tgccatcgtg gcgaaagcgc gtggctacga tatgaaagtc
     9301 attgccgtgg atgaccagtt tgttaacgcc aaaggtaagc caatggatac cgttcctctg
     9361 gtgatgatgg cggcgaccaa aattggcgaa cgtcagggcc aggaactgta taaagagatg
     9421 cagaaacgtg gctgggatgt caaagaaagc gcggtgatgg cgattacctc caacgaactg
     9481 gatacggctc gtcgccgtac tacgggttct atggatgcgc tgaaagcggc aggattcccg
     9541 gaaaaacaaa tttatcaggt acctaccaaa tctaacgaca tccccggggc atttgacgct
     9601 gccaactcaa tgctggttca acatccggaa gttaaacatt ggctgatcgt cggtatgaac
     9661 gacagcaccg tgctgggcgg cgtacgcgcg acggaaggtc agggctttaa agcggccgat
     9721 atcatcggta tcggtattaa cggtgtggat gcggtgagcg aactgtctaa ggcacaggca
     9781 accggcttct acggttccct gttgccaagc ccggacgtac atggttataa gtccagcgaa
     9841 atgctttaca actgggtagc aaaaggtgtt gaaccgacca aattcaccga agttaccgac
     9901 gtggtgctga tcacgcgtga caactttaaa gaagaactgg agaaaaaagg tttaggcggt
     9961 aagtaatttg ccggaaaaat tcccctctgc atgatgcaga gggggtgtga acgaccagtg
    10021 attcacggag acgttatgca acagtctacc ccgtatctct catttcgcgg catcggtaaa
    10081 acgtttcccg gcgttaaggc gctgacggat attagttttg actgctatgc cggtcaggtt
    10141 catgcgttga tgggtgaaaa tggcgcagga aaatcaactc tcttaaaaat cctcagcggc
    10201 aactatgcgc caaccacggg ttctgtagtg attaatgggc aggaaatgtc cttttccgac
    10261 acgaccgcag cacttaacgc gggtgtggcg attatttacc aggaactgca tctcgtgccg
    10321 gaaatgaccg tcgcggaaaa catctatctc ggccagctgc cgcataaagg cggtattgtg
    10381 aatcgctcat tgctgaatta tgaggcgggt ttacaactta aacatcttgg tatggatatt
    10441 gacccggaca cgccgctgaa atatctctcc attggtcagt ggcagatggt tgaaatcgcc
    10501 aaggcgctgg cgcgtaacgc taaaattatc gcctttgatg agccaaccag ctccctttct
    10561 gcccgcgaaa tcgacaatct tttccgcgtt attcgtgaac tgcgaaaaga ggggcgggtg
    10621 atcttatacg tttctcaccg tatggaagaa atatttgccc tcagcgatgc catcactgtc
    10681 tttaaagatg gacgttatgt caaaaccttt accgatatgc agcaggttga ccacgacgcg
    10741 ctggtgcagg cgatggtcgg gcgcgacatt ggcgatatct acggctggca accgcgtagt
    10801 tatggcgagg agcgcctgcg tcttgatgct gtgaaagcac caggcgtgcg tacgccaata
    10861 agtctggcgg ttcgcagtgg tgaaattgtt gggctgtttg gtctggtagg ggcggggcgt
    10921 agcgaattaa tgaaaggcat gtttggcggg acgcaaatca ccgccggtca ggtttatatc
    10981 gaccaacagc cgatcgatat tcgtaaaccg agccacgcca ttgccgcagg catgatgctc
    11041 tgcccggaag atcgcaaagc ggaaggcatt attcccgtgc actccgttcg cgacaatatc
    11101 aacatcagtg ccagacgtaa acatgtgctc ggcggttgtg taatcaacaa cggttgggaa
    11161 gaaaacaatg ccgatcacca cattcgttcg ctcaacatca aaacgccggg cgcggagcaa
    11221 ctgatcatga atctctcagg cggaaatcag caaaaagcca ttctgggccg ctggttatcg
    11281 gaagagatga aggtcatttt gctggatgaa cctacgcgcg gcattgatgt tggtgctaag
    11341 cacgaaatat ataacgtaat ttatgcgctg gcggcgcagg gtgtggcggt gctgtttgcc
    11401 tccagcgact tacctgaagt cctcggcgtt gccgaccgga ttgtggtgat gcgggaaggt
    11461 gaaatcgccg gtgaattgtt acacgagcag gcagatgagc gtcaggcact gagccttgcg
    11521 atgcctaaag tcagccaggc agttgcctga gtaaggagag tatgatgtct tctgtttcta
    11581 catcgggttc tggcgcacct aagtcgtcat tcagcttcgg gcgtatctgg gatcagtacg
    11641 gcatgctggt ggtgtttgcg gtgcttttta tcgcctgtgc catttttgtc ccgaactttg
    11701 ccaccttcat taatatgaaa gggttgggcc tggcaatttc catgtcgggg atggtggctt
    11761 gtggcatgtt gttctgcctt gcttccggtg actttgacct ttctgtcgcc tccgtaattg
    11821 cctgtgcggg tgtcaccacg gcggtggtta tcaacctgac tgaaagcctg tggattggcg
    11881 tggcagcggg gttgctgctt ggcattctct gtggcctggt caatggcttt gttatcgcca
    11941 aactgaaaat aaatgctctg atcacgacac tggcaacgat gcagattgtt cgaggtctgg
    12001 cgtacatcat ttcagacggt aaagcggtcg gtatcgaaga tgaaagcttc tttgcccttg
    12061 gttacgctaa ctggttcggt ctgcctgcgc caatctggct caccgtcgcg tgtctgatta
    12121 tctttggttt gctgctgaat aaaaccacct ttggtcgtaa taccctggcg attggcggga
    12181 acgaagaggc tgcgcgtctg gcgggtgtac cggttgttcg caccaaaatt attatctttg
    12241 ttctctctgg cctggtatct gcgatagccg gaattattct cgcttcacgt atgaccagtg
    12301 ggcagccaat gacgtcgatt ggttatgagc ttattgttat ctccgcctgc gttttaggtg
    12361 gcgtttctct gaaaggcggc atcggaaaaa tctcatatgt ggtggcgggt atcttaattt
    12421 taggcaccgt ggagaacgcc atgaacctgc ttaatatttc tcctttcgcg cagtacgtgg
    12481 ttcgcggctt aatcctgctg gcagcggtga tcttcgaccg ttacaagcaa aaagcgaaac
    12541 gcactgtctg atgctttttt ccacaacaat ttaacgtttt ttcccaccac agccagccgc
    12601 cacaacggtt ggctgttctt cgttgcaaat ggcgaccccc gtcacactgt ctatacttac
    12661 atgtctgtaa agcgcgttct gcgcaacaca ataagaaaag agaaggagga gaaccgggtg
    12721 acagaaccgt taaccgaaac ccctgaacta tccgcgaaat atgcctggtt ttttgatctc
    12781 gatggaacgc tggcggaaat caaaccgcat cccgatcagg tcgtcgtacc tgacaatatt
    12841 ctgcaaggac ttcagctact ggcaaccgca agtgatggtg cattggcatt gatatcaggg
    12901 cgctcaatgg tggagctgga cgcactggca aaaccttatc gcttcccgtt agcgggcgtg
    12961 catggggcgg agcgccgtga catcaatggt aaaacgcata tcgttcatct gccggatgcg
    13021 attgcacgtg atattaacgt gcaactgcat acggtcatcg cacagtatcc cggtgcggag
    13081 ctggagacga aagggatggc ttttgcgctg cattatcgtc aggctccgca gcatgaagac
    13141 gcattaatga cattagcgca acgtattact cagatctggc cacaaatggc gttacagcag
    13201 ggaaagtgtg ttgtcgagat caaaccgaga ggtaccagta aaggtgaggc gattgctgcc
    13261 tttatgcagg aagctccctt tatcgggcga acgcccgtat ttctgggcga tgatttaacc
    13321 gatgaatctg gtttcgcagt cgttaaccga ctgggcggaa tgtcagtaaa aattggcaca
    13381 ggcgcaactc aggcatcatg gcgactggcg ggcgtaccgg atgtctggag ctggcttgaa
    13441 atgataacca ccgcattaca acaaaaaaga gaaaataaca ggagtgatga ctatgagtcg
    13501 tttagtcgta gtatctaacc ggattgcacc accagatgag cacgccgcca gtgccggtgg
    13561 ccttgccgtt ggcatactgg gggcactgaa agccgcaggc ggactgtggt ttggctggag
    13621 tggtgaaaca gggaatgagg atcagccgct aaaaaaggtg aaaaagggta acattacgtg
    13681 ggcctctttt aacctcagcg aacaggacct tgacgaatac tacaaccaat tctccaatgc
    13741 cgttctctgg cctgcttttc attatcgcct cgatctggtg caatttcagc gtcctgcctg
    13801 ggacggctat ctacgcgtaa atgcgttgct ggcagataaa ttactgccgc tgttgcaaga
    13861 cgatgacatt atctggatcc acgattatca cctgttgcca tttgcgcatg aattacgcaa
    13921 acggggagtg aataatcgca ttggtttctt tctgcatatt cctttcccga caccggaaat
    13981 cttcaacgcg ctgccgacat atgacacctt gcttgaacag ctttgtgatt atgatttgct
    14041 gggtttccag acagaaaacg atcgtctggc gttcctggat tgcctttcta acctgacccg
    14101 cgtcacgaca cgtagcgcaa aaagccatac agcctggggc aaagcgtttc gaacagaagt
    14161 ttacccgatt ggcattgagc caaaagaaat agccaaacag gctgccgggc cactgccgcc
    14221 aaaactggcg caacttaaag cggaactgaa aaacgtacaa aatatctttt ctgtcgaacg
    14281 gctggattat tccaaaggtt tgccagagcg ttttctcgcc tatgaagcgt tgctggaaaa
    14341 atatccgcag catcatggta aaattcgtta tacccagatt gcaccaacgt cgcgtggtga
    14401 tgtgcaagcc tatcaggata ttcgtcatca gctcgaaaat gaagctggac gaattaatgg
    14461 taaatacggg caattaggct ggacgcctct ttattatttg aatcagcatt ttgaccgtaa
    14521 attactgatg aaaatattcc gctactctga cgtgggctta gtgacgccac tgcgtgacgg
    14581 gatgaacctg gtggcaaaag agtatgttgc cgctcaggac ccagccaatc cgggcgttct
    14641 tgttctttcg caatttgcgg gagcggcaaa cgagttaacg tcggcgttaa ttgttaaccc
    14701 ctacgatcgt gacgaagttg cagctgcgct ggatcgtgca ttgactatgt cgctggcgga
    14761 acgaatttcc cgtcatgcag aaatgctgga cgttatcgtg aaaaacgata ttaaccactg
    14821 gcaggagtgc ttcattagcg acctaaagca gatagttccg cgaagcgcgg aaagccagca
    14881 gcgcgataaa gttgctacct ttcctaagct tgcgtaggag cggttaatct cccgtaagtg
    14941 gaactaacag cacatcgacc tcacttgagg caatcactct tttcgccgag caggatgctc
    15001 gcgaaaagaa actgtgattg tgattaccgc aaatcaccaa atcgaaatga tatttgcgac
    15061 aaacttccag aatatgttcg cttaactcac cgtaggcaat aaacgtcttg tcaacgggat
    15121 accccgcatc ctgaattaac ttatcaagaa agctttgcgt ttcttcctgc atcacgctgc
    15181 gtaaatcttc aagcatcggc gcagctaatt gattgtacat ttccgggtcg gaagcgagag
    15241 taattaaact gatgtgtccc tttactggcc tggcgataga taccgctttt gccagcagtt
    15301 gctgactttc cggtgtaacc gcaacagcga caagaatatt gctatagctc ataacctgtt
    15361 ccttattctg tgaacttcag gtgacattaa agctttactc ctaatggaga aacgacgcaa
    15421 tcccaactca gtcaaacagg taaatattag tctgattatt agtaaagaca attaatcaat
    15481 cactctcggt gatcatatga tttcaactgt aattataaga aaataatgta ctgattcccc
    15541 gcattaaaca agtggttaat atatgcaaaa cattttatta acattaag
//
LOCUS       DAZUCE010000112        15478 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZUCE010000112 DAZUCE010000000
VERSION     DAZUCE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.112"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            18..254
                     /gene="yjjZ"
                     /locus_tag="ACHY03_002980"
     CDS             18..254
                     /gene="yjjZ"
                     /locus_tag="ACHY03_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFS3906185.1"
                     /translation="MLQRTLGSGWGVLLPGFLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALSAGIMLMLMNLNQG"
     gene            complement(293..379)
                     /locus_tag="ACHY03_002981"
     tRNA            complement(293..379)
                     /locus_tag="ACHY03_002981"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(343..345),aa:Leu,seq:cag)
     gene            complement(647..1678)
                     /gene="rsmC"
                     /locus_tag="ACHY03_002982"
     CDS             complement(647..1678)
                     /gene="rsmC"
                     /locus_tag="ACHY03_002982"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFS3906186.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHMKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1781..2194
                     /gene="holD"
                     /locus_tag="ACHY03_002983"
     CDS             1781..2194
                     /gene="holD"
                     /locus_tag="ACHY03_002983"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFS3906187.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            2163..2609
                     /gene="rimI"
                     /locus_tag="ACHY03_002984"
     CDS             2163..2609
                     /gene="rimI"
                     /locus_tag="ACHY03_002984"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFS3906188.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2624..3301
                     /gene="yjjG"
                     /locus_tag="ACHY03_002985"
     CDS             2624..3301
                     /gene="yjjG"
                     /locus_tag="ACHY03_002985"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFS3906189.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3392..4981
                     /gene="prfC"
                     /locus_tag="ACHY03_002986"
     CDS             3392..4981
                     /gene="prfC"
                     /locus_tag="ACHY03_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFS3906190.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5374..5979
                     /gene="osmY"
                     /locus_tag="ACHY03_002987"
     CDS             5374..5979
                     /gene="osmY"
                     /locus_tag="ACHY03_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFS3906191.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            6106..6267
                     /gene="ytjA"
                     /locus_tag="ACHY03_002988"
     CDS             6106..6267
                     /gene="ytjA"
                     /locus_tag="ACHY03_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFS3906192.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6389..7462
                     /gene="yjjU"
                     /locus_tag="ACHY03_002989"
     CDS             6389..7462
                     /gene="yjjU"
                     /locus_tag="ACHY03_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFS3906193.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7459..8241
                     /gene="yjjV"
                     /locus_tag="ACHY03_002990"
     CDS             7459..8241
                     /gene="yjjV"
                     /locus_tag="ACHY03_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS3906194.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAA
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRREPVNEIAEVLLNNTYAVFNVRG"
     gene            complement(8455..9318)
                     /gene="yjjW"
                     /locus_tag="ACHY03_002991"
     CDS             complement(8455..9318)
                     /gene="yjjW"
                     /locus_tag="ACHY03_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFS3906195.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
     gene            complement(9290..10840)
                     /gene="yjjI"
                     /locus_tag="ACHY03_002992"
     CDS             complement(9290..10840)
                     /gene="yjjI"
                     /locus_tag="ACHY03_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFS3906196.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDVLLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            11098..11877
                     /gene="deoC"
                     /locus_tag="ACHY03_002993"
     CDS             11098..11877
                     /gene="deoC"
                     /locus_tag="ACHY03_002993"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFS3906197.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            12004..13326
                     /gene="deoA"
                     /locus_tag="ACHY03_002994"
     CDS             12004..13326
                     /gene="deoA"
                     /locus_tag="ACHY03_002994"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFS3906198.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     IDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            13378..14601
                     /gene="deoB"
                     /locus_tag="ACHY03_002995"
     CDS             13378..14601
                     /gene="deoB"
                     /locus_tag="ACHY03_002995"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFS3906199.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14658..15377
                     /gene="deoD"
                     /locus_tag="ACHY03_002996"
     CDS             14658..15377
                     /gene="deoD"
                     /locus_tag="ACHY03_002996"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFS3906200.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3677 a         4014 c         4211 g         3576 t
ORIGIN      
        1 atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag tggctgggga gtgttgctgc
       61 cgggatttct gattgcaggg ctgatgtatg cggatttatc gccggatcag tggcggattg
      121 tcattctgat gggattgata ttgacgccgg tgatgctgta tcacaaacag ttacggcatt
      181 acgttttgct accatcgtgc ctggcgctta gtgctggcat catgctgatg ctaatgaatt
      241 tgaatcaggg atgacaaaac agggaagcaa caagaaagga agtaaagata attggtgcga
      301 ggggggggac ttgaaccccc acgtccgtaa ggacactaac acctgaagct agcgcgtcta
      361 ccaattccgc caccttcgca taccatcaat tcttaaaaag aattgctacc acggaggcgc
      421 attctagtgg ttttcagctt ttcgtcaata gttaattatc gacagaggtg taattgctgg
      481 aaaaatgtcc atcaggaaac tagcgtgcag gtttggtagg catgcggggg cagatgccag
      541 atgcgacgct ggcgcgtctt atctggccta cgaagggcta acgtgcaggt tttgtaggtc
      601 ggataaggcg ttcacgccgc atccgacacg gtattcggcg agataattaa cctttcttcg
      661 cctggcgggt cataatggcg cgatacacct tgaagcgccc ggtttgcgcg ataacttcat
      721 ggaagccaaa tgtctcatcc agcacgtccg ggtaaggcag gaaggcgttc gctacaattc
      781 gcagctcgcc gccactatta agatgacgca ccgcgccgcg aatcagcgtt tgcgccgcat
      841 ccaggctggt ttgcatccca tcgtggaacg gcgggttgga gatgatcata tcaaaacgac
      901 ctttcacctc ggaaaagacg ttgctggcaa agacttcacc ttcaacaccg ttggccgcaa
      961 gtgttgcgcg gctggcttct accgccgggg cagagacatc gcacaaggtg agacgaattt
     1021 tcggcgaatg gcgcgcaaag gcaactgaaa gtacacccgc gccacagccg acatccagca
     1081 ctttaccttt catgtgcgga gttaacgtcg agagcagcaa ctggctaccg acatccagac
     1141 cgtcgcggct aaacacgcca ggcagcgttt tgaccgtcag gccatcgacg ctgtattcgc
     1201 cccagaattt atcggcatca aataccggct gtttttccag acgaccaaaa tagaggccac
     1261 agcgacgagc gctgtcgact ttattcaacg gcgcataatc tgccagcatc tgctcggcgc
     1321 tgcgcacgcc gctgcggttc tcgccaacaa caaaaatatc cgtccccacc ggaagcagag
     1381 aaagtaaatt catcaactgg aactgggctt ccggtttgtt cttcggccag tagtaaatca
     1441 gcgtatcgca atctgcgacg tcatccgccg tggcgaccag actaaaacgg gcgttatccc
     1501 ccatctggcg gcttaacacc tgccagtggt ggaattgctg ggtatgagca cggctggccg
     1561 cggtatctaa acgcgcgggc aggtcatcct gtaagtctcc ggcaaacaga atacggcttt
     1621 gttcgaaatc atcactgtga cgcagcaaga cttcacttgc cggggtaaat gcagacatgg
     1681 aatgctcctc aattgatact ggcggcgatt atagccatat gttggcgcgg tatcgacgaa
     1741 tttgctatat ttgcgcccct gacaacagga gcgattcgct atgacatccc gacgagactg
     1801 gcagttacag caactgggca ttacccagtg gtcgctgcgt cgccctggcg cgttgcaggg
     1861 ggagattgcc attgcgatcc cggcacacgt ccgtctggtg atggtggcaa acgatcttcc
     1921 cgccctgact gatcctttag tgagcgatgt tctgcgtgca ttaaccgtca gccccgacca
     1981 ggtgctgcaa ctgacgccag aaaaaatcgc gatgctgccg caaggcagtc gctgcaacag
     2041 ctggcggttg ggtactgacg aaccgctatc actggaaggc gctcaggtgg catcaccggc
     2101 gctcaccgaa ttacgggcaa acccaacggc acgcgccgcg ttatggcaac aaatttgcac
     2161 atatgaacac gatttcttcc ctcgaaacga ctgatttacc agcggcttac cacattgaac
     2221 aacgcgccca cgcctttccg tggagtgaaa aaacctttgc cagcaaccag ggcgagcgtt
     2281 atctcaactt tcagttaacg caaaacggca aaatggcggc gtttgcgatt acgcaagtgg
     2341 tgctggatga agctacattg ttcaatattg cggtcgatcc tgactatcag cgtcagggat
     2401 tgggaagggc gctgctggaa catctgatcg acgaactgga aaaacgcggc gtggcgacgc
     2461 tctggctgga agtccgtgcc tcaaacgctg ccgccattgc cctgtacgaa agtttaggct
     2521 ttaacgaggc gacgattcgc cgcaattact accccaccac ggacggtcgc gaagacgcca
     2581 tcatcatggc gttgccaatc agtatgtaag acaaggtgga ataatgaagt gggactggat
     2641 tttctttgat gccgatgaaa cgctgtttac ctttgactca ttcaccggcc tgcagcggat
     2701 gtttcttgat tacagcgtca cctttaccgc tgaagatttt caggactatc aggccgttaa
     2761 caagccactg tgggtggatt atcaaaacgg cgcgatcact tcattacagc ttcagcacgg
     2821 gcggtttgag agctgggccg aacggctgaa cgtcgagccg ggtaaactca acgaagcctt
     2881 tattaatgcg atggcggaaa tctgtacgcc gctgccgggc gcggtttctc tgcttaacgc
     2941 cattcgtggc aacgccaaaa tcggcatcat caccaacggt tttagcgcct tgcagcaagt
     3001 gcgtctggaa cggacgggcc tgcgtgatta cttcgatttg ctggtgattt ccgaagaagt
     3061 tggcgttgct aaaccgaata agaaaatttt cgattatgcg ctggaacagg cgggcaatcc
     3121 tgaccgttca cgcgtgctga tggttggcga cactgccgag tccgatattc tcggtggcat
     3181 caacgccggg cttgcgacct gctggctgaa tgcacaccat cgcgagcaac cagaaggcat
     3241 cgcgcccacc tggaccgttt cttcgttgca cgaactggag cagctcctgt gtaaacactg
     3301 attgcctccc ccccgttgat gggtaaaata gccgcaattt ttcgttttca acaagcgcgg
     3361 cgcgatgccg cttactcaag aagaaagaat tatgacgttg tctccttatt tgcaagaggt
     3421 ggcgaagcgc cgcacttttg ccattatttc tcacccggac gccggtaaaa ctaccatcac
     3481 cgagaaggtg ctgctgttcg gacaggccat tcagaccgcc ggtacagtaa aaggccgtgg
     3541 ttccaaccag cacgctaagt cggactggat ggagatggaa aagcagcgtg ggatctccat
     3601 tactacgtct gtgatgcagt ttccgtatca cgattgcctg gttaacctgc tcgacacccc
     3661 ggggcacgaa gacttctcgg aagataccta tcgtaccctg acggcggtgg actgctgcct
     3721 gatggttatc gacgccgcaa aaggtgttga agatcgtacc cgtaagctga tggaagttac
     3781 ccgtctgcgc gacacgccga tcctcacctt tatgaacaaa cttgaccgtg atatccgcga
     3841 cccgatggaa ttgctcgatg aagttgagaa cgagctgaaa attggctgcg caccgatcac
     3901 ctggccaatt ggctgcggca agctgtttaa aggcgtttac cacctttata aagatgaaac
     3961 ctatctctat cagagcggta aagggcacac cattcaggaa gtccgcattg ttaaagggct
     4021 gaataacccg gatctcgatg ctgcggttgg tgaagatctg gcacagcagc tgcgtgacga
     4081 actggaactg gttaaaggcg cgtctaacga gttcgacaaa gagctgttcc ttgcgggcga
     4141 aatcactccg gtattcttcg gtactgcgct gggtaacttc ggcgttgatc atatgctgga
     4201 tggcctggtg gagtgggcgc ctgcgccgat gccgcgtcag accgataccc gtaccgtaga
     4261 agcgagcgaa gataaattta ccggcttcgt atttaaaatt caggccaaca tggacccgaa
     4321 acaccgcgac cgcgtggcgt ttatgcgtgt ggtgtccggt aaatatgaaa aaggcatgaa
     4381 actgcgccag gtgcgcactg cgaaagatgt ggtgatctcc gacgcgctga cctttatggc
     4441 gggtgaccgt tcgcacgttg aagaagcgta tccgggcgat atcctcggcc tgcacaacca
     4501 cggcaccatt cagatcggcg acacctttac ccagggtgag atgatgaagt tcaccggtat
     4561 tccgaacttc gcaccagaac tgttccgtcg tatccgcctg aaagatccgc tgaagcaaaa
     4621 acagctgctc aaagggctgg tacagctttc cgaagagggc gcggtgcagg tgttccgtcc
     4681 aatctccaac aacgatctga tcgttggtgc agttggtgtg ctgcagtttg atgtggtggt
     4741 agcgcgcctg aagagcgaat acaacgttga agcagtgtat gagtcagtca acgttgccac
     4801 tgcccgctgg gtagaatgtg cagacgcgaa gaaattcgaa gagttcaagc gtaagaacga
     4861 aagccaactg gcgcttgatg gcggcgataa cctcgcttac atcgctacca gcatggtcaa
     4921 cctgcgcctg gcacaggaac gttatccgga cgttcagttc caccagaccc gcgagcatta
     4981 attcttgcct ccagggcgcg gtagccgctg cgccctgtca atttcccttc cttattagcc
     5041 gcttacggaa tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg
     5101 gtttcaaaat tgtgatctat atttaacaaa gtgatgacat ttctgacggc gttaaatacc
     5161 gttcaatgcg tagatatcag tatctaaagc cgtcgattgt cattctaccg atattaataa
     5221 ctgattcaga ggctgtaatg gtcgttattc atcactcatc gcttttgtga tggcgaccat
     5281 tgacttctgt agagggtgaa gtctctccct attcagcaat gcaacctcgt gttgccaggc
     5341 tcaaattacg agcaaacata caggaataaa tcgatgacta tgacaagact gaagatttcg
     5401 aaaactctgc tggctgtaat gttgacctct gccgtcgcga ccggctctgc ctacgcggaa
     5461 aacaacgcac aaactaccaa tgaaagcgca gggcaaaaag tcgatagctc tatgaataaa
     5521 gtcggtaatt tcatggatga cagcgccatc accgcgaaag tgaaggcggc gctggtggat
     5581 catgacaaca tcaagagcac cgatatctct gtcaaaaccg atcaaaaagt cgtgaccctg
     5641 agcggtttcg ttgaaagtca ggcccaggcc gaagaggcag tgaaagtggc gaaaggcgtt
     5701 gaaggcgtga cctctgtcag tgacaaactg cacgttcgcg acgctaaaga aggctctgtg
     5761 aagggctacg caggtgacac tgccaccacc agtgaaatca aagccaaact gctggcggac
     5821 gatatcgtcc cttcccgtca tgtgaaagtt gaaaccaccg atggcgtggt tcagctctcc
     5881 ggtaccgtag attctcaggc acaaagtgac cgtgctgaaa gtatcgccaa agcggtagat
     5941 ggtgtgaaaa gcgttaaaaa tgatctgaaa actaagtaat tcgtcgtaat tcgtcctccc
     6001 gaactttgtc gggaggcgcg atgtgcacca cactaaaaat atcgcattga gtgattttca
     6061 cgctcatatc aagcggtgac attaactatg gtaaaggaga cgcttatgtt tcgttggggc
     6121 atcatatttc tggttatcgc gttaatcgcc gccgcacttg ggtttggtgg tctggccggt
     6181 accgctgcag gcgcagctaa aattgtcttt gtcgtcggga ttattctgtt cctggtgagt
     6241 ttgttcatgg gccgaaaacg accctagatt tcagaccatt aagaaaattt tatccaaagc
     6301 cagtccagcg gactggcttt tgcggtttta gcgaataata aattgcgtta ctttgtcttt
     6361 ctttcacaac aaaaacagga aggcagaagt ggggcagcga atacctgtaa cgcttggtaa
     6421 tattgcgccg ttgtcgctaa ggccgttcca gcctggacga atagctctgg tgtgcgaagg
     6481 cggcgggcag cgtggaattt tcacggctgg cgtgctggat gagtttatgc gcgcgcagtt
     6541 taatcctttc gatctttatc tcggcacatc tgccggggcg cagaacctct cggcgtttat
     6601 ctgcaatcag cccggttacg cgcgcaaagt catcatgcgc tataccacaa aacgcgaatt
     6661 tttcgatcca ttgcgctttg tccgtggagg aaatcttatc gatctcgact ggctggtgga
     6721 ggccactgca agccagatgc cgttgcaaat ggataccgcc gcgcggttgt ttgacagcgg
     6781 caaatcgttt tatatgtgcg cctgtcgtca ggatgactac gcgccgaatt actttttacc
     6841 aaccaaacaa aactggctgg atgtgattcg cgcctccagt gcgatacctg gcttttatcg
     6901 tagcggagtg tcgctggaag gcattaacta cctggatggc gggatcagtg atgcgattcc
     6961 ggttaaagag gcggcaaggc agggcgctaa aacgttggtc gtcatccgca ctgtgccgtc
     7021 gcaaatgtac tatacgccgc agtggtttaa acgcatggaa cgctggctgg gcgacagtag
     7081 cctgcaaccg ctggtcaatc tggtacagca tcatgaaacc agctatcgtg acattcagca
     7141 atttattgag aaaccaccgg gcaagctgcg gatattcgaa atttatccgc cgaagccatt
     7201 acatagtatc gcgcttggca gtcggattcc ggcgctgcgc gaagactata aacttgggcg
     7261 tttatgcggt cgttatttcc tcgccacggt tggcaagcta ttaactgaaa aagcgccgct
     7321 tacccgccat ctggtgccag tggtgacgcc ggaatcgatt gtcattccgc ctgcgccagt
     7381 cgccaacgat acactggttg ccgaagtgag cgacgctccg caggcgaacg acccgacatt
     7441 taacaatgag gatctggctt gatttgccgt tttatcgaca ctcactgcca ttttgatttt
     7501 ccgccgttta gcggtgatga agaggctagc ctgcaacgcg cggcacaagc gggcgtaggc
     7561 aagatcattg ttccggcaac cgaggcggca aattttgccc gcgtgcaggc gttagcggaa
     7621 aattatcaac cgctgtatgc cgcattgggc ttgcatcctg gtatgttgga aaaacatagc
     7681 gatgtgtctc ttgagcagct acagcaggcg ctggaaaggc gtccggcgaa ggtggtggcg
     7741 gtgggggaga tcggtctgga tctctttggc gacgatccgc aatttgagag gcagcagtgg
     7801 ttactcgacg aacaactgaa actggcgaaa cgctacgatc tgccggtgat cctgcattca
     7861 cggcgcacac acgacaaact ggcgatgcat cttaaacgcc acgatttatc gcgcactggc
     7921 gtggttcacg gtttttccgg cagcctgcaa caggccgagc ggtttgtaca gctgggctac
     7981 aaaatcggcg taggcggtac tatcacctat ccacgcgcca gtaaaacccg cgatgtcatc
     8041 gcaaaattac cgctggcatc gttgttgctg gaaaccgatg cgccggatat gccgctcaac
     8101 ggttttcagg ggcagcctaa ccgcccggaa caggctgccc gtgtgtttgc cgtgctttgc
     8161 gagttgcgcc gggaaccggt gaatgagatt gccgaagtgt tgcttaataa cacgtatgcg
     8221 gtgtttaacg ttcgtgggta gtttgccctt cgtaggcagc atccggcagt cggcgcataa
     8281 tgcctgatgc gacgcttaac gcgtcttatc atgcctacag gtttgtgccg aaccgtaggc
     8341 cggataaggc gttcacgccg catccggcag ttggcgcaca atgcctgatg cgacgcttga
     8401 cgcgtcttat caggcctaca agtctgtgcc gaaccgtagg ccgtatccgg catgtcacaa
     8461 atacagcgcc ggaaatatta accggctcac cccgcgctcc cgtaacgcat cagccaacgg
     8521 ctcaacgtct tccggcgtgg cgctcgccca gctttgcgcc tcgccataca cgccgtgggc
     8581 atgaaacgcg ttcaggcgta ccggaacatc gccaagtccc ttgataaaca ccgccagttc
     8641 ttcgatgtgt tgcaaataat ccacctggcc agggattacc agcaaacgca gttccgccag
     8701 cttgccgcgc tctgccagca aacagatgct gcgcttaatc tgctgattat cgcgtccggt
     8761 taattgttga tgacattcgc tcccccacgc tttgagatcg agcattgcgc cgtcgcacac
     8821 cgggagcaat ttttcccagc cggtttcgct caacataccg ttactgtcca cgaggcaggt
     8881 gagatggcac agttgcggat cgtttttgat ggcagtaaac agcgccacta caaacggcag
     8941 ttgggtcgtg gcttcgccgc cgcttaccgt tatcccttca ataaacagca ccgctttgcg
     9001 gatatggcta agcacctcat ccacgctcat ggattgcgcc attggcgtgg catgttgcgg
     9061 acacctcttc aggcaggtat cacattgctc gcagaccaca gcgttccaca ccactttgcc
     9121 gtcaacaatc tgcaacgcct gatgcggaca ctgtggcacg cactccccac agtcattgca
     9181 acgtcccatc gtccacggat tgtgacagtt tttgcagcgc agattgcagc cctgcaaaaa
     9241 cagagccaga cgactgcctg gcccgtcaac gcaggagaag gggataatct tactgactaa
     9301 agcgcatctg ctgttcatgg cttatcacgc gcggctggcg ttccagaata cgagtgttgc
     9361 gtgcggcttc ttcgcccagc caggtggtgt tggtgcgtga accttcggcg cgatattttt
     9421 ctaaatccga caaacgcacc atataaccgg taacgcgaac cagatcgtta ccgctgacat
     9481 tggcggtaaa ttcacgcatt ccggctttaa aggcaccgag gcaaagctgt accagcgcct
     9541 gcggattacg tttgatggtt tcgtcgagcg tcagaatgtc gctgatgccg gaataataat
     9601 aagcatgatg cggggcgaca gtttgcagat gggtgatcgg atctggctca tcgccatacg
     9661 gcaaacgcgc gcccggcgtg gtgccgatat cggaactgat ccccgactgt gcgtgtaaca
     9721 tggcgcgttt ttgccagcca tatttcacgg gggtattggc gacaaactcc gccagttgcg
     9781 cgctgatgcg ataacccact tcatttgcgg cggcttcttt accgtagcgc gcggcaatcc
     9841 cttctttctc acacagcaag ttaaccgctt ccgccagccc atacatgcca aacattggca
     9901 caaaacgttc agggttaatc agcccttctt tcaccaggaa gctattctca aagaagtgtg
     9961 attgttgata gaggaattca caccgcgcat cgatgatggc gatctgctgc tggcagtagt
    10021 gcggtagagt gcgcgtaaag aagtcatcca gcgattcgct gcgctcggca atggctttca
    10081 ggttaaggcg taccagcgtg ctgccaccac ccgccagcgg cagtgagttg taacagctca
    10141 caatcccgta gccccctttt gtgaaaattt tatcatgcac cggaccgttg gcgatgtgcg
    10201 gtttgctaca ttcacagatg ttcttcgcca cttccagcag caggtcatca ggggtgattt
    10261 caggatcgta gataaaggtc aggttcggtg aaacctgctt caactctgcg tctgcacgta
    10321 agattgcacg ggtaatgggc gaatcagacg ggccgatatt ggcgtgcata aaggcgtctg
    10381 gcagggttct gtcgaggtaa cgccagaaac gttttattcg aatatcgatc tcgtcttgtg
    10441 ttagaattct aacatacggt tgcaacaaca catccagttg ccccaggtag accggcatcg
    10501 atgtgaccga cggtacgtgg tggtaaagaa tggtcagcag agagagtgcg tcatcaagat
    10561 ctttcgcgcc ttccagctcc agccattcgg aaccgttcgc cagaaaacgg gcgtaatcgg
    10621 gtaagacata gcgcggtttg tacggcgcat gaccttcaaa catatcgcag attacacctt
    10681 catccagcgc gcggcgggct tcggcaggaa gctgtgggta aggcagattg ttttctgctt
    10741 ccagtgccag aaaatggcgc ttctgctccg ggctaagaac tgggctggtg acaatttgct
    10801 ggcaacgttg ttgcagtgca ttttcatgag aagtgggcat cttcttttcc ttttatgccg
    10861 aaggtgatgc gccattgtaa gaagtttcgt gatgttcact ttgatcctga tgcgtttgcc
    10921 accactgacg cattcatttg aaagtgaatt atttgaacca gatcgcatta cagtgatgca
    10981 aacttgtaag tagatttcct taattgtgat gtgtatcgaa gtgtgttgcg gagtagatgt
    11041 tagaatacta acaaactcgc aaggtgaatt ttattggcga caagccagga gaatgaaatg
    11101 actgatctga aagcaagcag cctgcgtgca ctgaaattga tggacctgac caccctgaat
    11161 gacgacgaca ccgacgagaa agtaattgct ctgtgtcatc aggccaaaac cccggtcggc
    11221 aataccgccg ctatctgtat ctatcctcgc tttatcccga ttgctcgcaa aacactgaaa
    11281 gagcagggca ccccggaaat ccgtattgct acggtaacca acttcccaca cggtaacgac
    11341 gacatcgaaa tcgcgctggc agaaacccgt gcggcaatcg cctacggtgc tgatgaagtt
    11401 gacgtggtgt ttccgtaccg cgcgctgatg gcgggtaacg agcaggttgg ttttgacctg
    11461 gtgaaagcct gtaaagaggc ttgcgcggca gcgaatgtac tgctgaaagt gatcatcgaa
    11521 accggcgaac tgaaagacga agcgctgatc cgtaaagcgt ctgaaatctc catcaaagcg
    11581 ggtgcggact tcatcaaaac ctctaccggt aaagtggctg tgaacgcgac gccggaaagc
    11641 gcgcgcatca tgatggaagt gatccgtgat atgggcgtag aaaaaaccgt tggtttcaaa
    11701 ccggcgggcg gcgtgcgtac tgcggaagat gcgcagaaat atctcgccat cgcagatgaa
    11761 ctgttcggtg ctgactgggc tgatgcgcgt cactaccgct ttggtgcttc cagcctgctg
    11821 gcaagcctgc tgaaagcgct gggtcacggc gacggtaaga gcgccagcag ctactaagta
    11881 agatgcttta cgcctgatgc gctgcgctta tcaggcctac gagacgtatc tacccgtagg
    11941 ccggataagg cgtagacgca tccggcaaaa gccgcctcat actcttttcc acgggaggtt
    12001 accttgtttc tcgcacaaga aattattcgt aaaaaacgtg atggtcatgc gctgagcgat
    12061 gaagaaattc gtttctttat caacggtatt cgcgacaaca ctatctccga agggcagatt
    12121 gccgccctcg cgatgaccat tttcttccac gatatgacaa tgcctgagcg tgtctcgctg
    12181 accatggcga tgcgagattc aggaaccgtt ctcgactgga aaagcctgca tctgaatggc
    12241 ccgattgttg ataaacactc gaccggcggc gtcggcgatg tgacttcgct gatgttgggg
    12301 ccgatggtcg cagcctgcgg cggctatatt ccgatgatct ctggtcgcgg cctcggtcat
    12361 actggcggta cgctcgacaa actggaatcc atccctggct tcgacatttt cccggatgac
    12421 aaccgtttcc gcgaaattat taaagacgtc ggcgtggcga ttatcggtca gaccagctca
    12481 ctggctccgg cggataaacg tttctacgcg acccgtgata ttaccgcaac cgtggactcc
    12541 atcccgctga tcaccgcctc tattctggcg aagaaacttg cggaaggtct ggacgcgctg
    12601 gtgatggacg tgaaagtggg tagcggcgcg tttatgccga cctacgaact ctctgaagcc
    12661 cttgccgaag cgattgttgg cgtggctaac ggcgctggcg tgcgcaccac cgcgctgctc
    12721 accgacatga atcaggtgct ggcctccagt gcaggtaacg cggttgaagt tcgtgaagcg
    12781 gtgcagttcc tgacgggtga atatcgtaac ccgcgtctgt ttgatgtcac gatggcgctg
    12841 tgcgtggaga tgctgatctc cggcaaactg gcgaaagatg acgccgaagc gcgcgcgaaa
    12901 ttgcaggcgg tgctggacaa cggtaaagcg gcagaagtct ttggtcgtat ggtagcggca
    12961 caaaaaggcc cgaccgactt cgttgagaac tacgcgaagt atctgccgac agcgatgctg
    13021 acgaaagcag tctatgctga taccgaaggt tttgtcagtg aaatggatac ccgcgcgctg
    13081 gggatggcag tggttgcaat gggcggtgga cgccgtcagg catctgacac catcgattac
    13141 agcgtcggct ttactgatat ggcgcgtctg ggcgaccaga tagacggtca gcgtccgctg
    13201 gcggttatcc acgcgaaaga cgaaaacagc tggcaggaag cggcgaaagc ggtgaaagcg
    13261 gcaattaaac ttgccgataa agcaccggaa agcacaccaa ctgtctatcg ccgtatcagc
    13321 gaataacggt atactgatct gatcatttaa atttgaagca ctgagtacgg agaacatatg
    13381 aaacgtgcat ttattatggt gctggactca ttcggcatcg gcgctacaga agatgcagaa
    13441 cgctttggtg acgtcggggc tgacaccctg ggtcatatcg cagaagcttg tgccaaaggc
    13501 gaagctgata acggtcgtaa aggcccgctc aatctgccaa atctgacccg tctggggctg
    13561 gcgaaagcac acgaaggttc taccggtttc attccggcgg gaatggacgg caacgctgaa
    13621 gttatcggcg cgtacgcatg ggcgcacgaa atgtcatccg gtaaagatac cccgtctggt
    13681 cactgggaaa tcgccggtgt cccggttctg tttgagtggg gatatttctc cgatcacgaa
    13741 aacagcttcc cgcaagagct gctggataaa ctggtcgaac gcgctaatct gccgggttac
    13801 ctcggtaact gccactcttc cggtacggtc attctggatc aactgggcga agagcacatg
    13861 aaaaccggca agccgatttt ctatacctcc gctgactccg tgttccagat tgcctgccat
    13921 gaagaaactt tcggtctgga taaactctac gaactgtgcg aaatcgcccg tgaagagctg
    13981 accaacggcg gctacaatat cggtcgtgtt atcgctcgtc cgtttatcgg cgacaaagcc
    14041 ggtaacttcc agcgtaccgg taaccgtcac gacctggctg ttgagccgcc agcaccgacc
    14101 gtgctgcaga aactggttga tgaaaaacac ggccaggtgg tttctgtcgg taaaattgca
    14161 gacatctacg ccaactgcgg tatcaccaaa aaagtgaaag cgactggcct ggacgcgctg
    14221 tttgacgcca ccatcaaaga gatgaaagaa gcgggtgata acaccatcgt cttcaccaac
    14281 ttcgttgact tcgactcttc ctggggccac cgtcgcgacg tcgccggtta tgctgcgggt
    14341 ctggaactgt tcgaccgccg tctgccggag ctgatgtctc tgctgcgcga tgacgacatc
    14401 ctgatcctca ccgctgacca cggctgtgat ccgacctgga ccggtactga ccacacgcgt
    14461 gaacacattc cggtactggt ttacggcccg aaagtaaaac cgggctcact gggtcaccgt
    14521 gaaaccttcg cggatatcgg ccagactctg gcaaaatatt ttggtacttc tgatatggaa
    14581 tatggcaaag ccatgttctg atggatttgg gcggagcgtt gactccgcct ttgttatgtc
    14641 acaaaaagga taaaacaatg gctaccccac acattaatgc agaaatgggc gatttcgctg
    14701 acgtagtttt gatgccaggc gacccgctgc gtgcaaagta tattgctgaa actttccttg
    14761 aagatgcccg tgaagtgaac aacgttcgcg gtatgctggg cttcaccggt acttacaaag
    14821 gccgcaaaat ttccgtaatg ggtcacggta tgggtatccc gtcctgctcc atctacacca
    14881 aagaactgat caccgatttc ggcgtgaaga aaattatccg cgtgggttcc tgtggcgcag
    14941 ttctgccgca cgtaaaactg cgcgacgtcg ttatcggtat gggtgcctgc accgattcca
    15001 aagttaaccg catccgtttt aaagaccatg actttgccgc tatcgctgac ttcgacatgg
    15061 tgcgtaacgc agtagatgca gctaaagcac tgggcgttga tgctcgcgtg ggtaacctgt
    15121 tctccgctga cctgttctac tctccggacg gcgaaatgtt cgacgtgatg gaaaaatacg
    15181 gcatcctcgg cgtggaaatg gaagcggctg gtatctacgg cgtggctgca gagtttggcg
    15241 cgaaagccct gaccatctgc accgtgtctg accacatccg cactcacgag caaaccactg
    15301 ccgctgagcg tcagaccacc ttcaacgaca tgatcaaaat cgcactggaa tccgttctgc
    15361 tgggcgataa agagtaattg tgtttcgctg aaaggcgatt gttgtgttaa gccggagcgg
    15421 gagactgctc cggcttttta gtatctattc atcaatagca actgatttat agatttac
//
LOCUS       DAZUCE010000113        15398 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZUCE010000113 DAZUCE010000000
VERSION     DAZUCE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.113"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(67..348)
                     /gene="yehE"
                     /locus_tag="ACHY03_002997"
     CDS             complement(67..348)
                     /gene="yehE"
                     /locus_tag="ACHY03_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFS3906201.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPAKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(611..1720)
                     /gene="apbC"
                     /locus_tag="ACHY03_002998"
     CDS             complement(611..1720)
                     /gene="apbC"
                     /locus_tag="ACHY03_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFS3906202.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1852..3885
                     /gene="metG"
                     /locus_tag="ACHY03_002999"
     CDS             1852..3885
                     /gene="metG"
                     /locus_tag="ACHY03_002999"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFS3906203.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4026..4220
                     /locus_tag="ACHY03_003000"
                     /pseudo
     CDS             4026..4220
                     /locus_tag="ACHY03_003000"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            6759..7835
                     /locus_tag="ACHY03_003001"
                     /pseudo
     CDS             6759..7835
                     /locus_tag="ACHY03_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7848..10880
                     /locus_tag="ACHY03_003002"
     CDS             7848..10880
                     /locus_tag="ACHY03_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFS3906204.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQRNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAELKLIAEKVKKGYVEQVDANAHSHDVSAKKVLVTEEKASINHQAATNGLPW
                     LADNDPIILPPNIARHALSHRLWPGEPVKKPTKPEKQNLISRLANRTYHYYKNLTFDY
                     SSCSDEWADAISQAISLIKAQKTEIPARTMAVLTFLEICFCQGWNDETSSELLDEIVS
                     TFGIEYATETVIFWWQIDFDFDYKDEHLTFFINYADSSKDSYRRNNDKFSLRLRKHLS
                     LAEEEVWQNCIAKLLVALPDISLYRQPLIAILMPEIPEVAHEIVHRLHQVADVPQLEM
                     LKLVATDPFTLEILENYQYIDVFNTYGASWSATVLREQGIAALARLASYAEEDNCGDV
                     LKCINHPLAITALIEAADTNKRCYERLIKSAENFPSATIAALAEALVKKDDKRKHTLL
                     VSLLNANPLLVEKLVIGLSSQAANLLQQCQQLLNSRNEDFANSEILPMVLVSPPWETQ
                     NKNNDNSPVGKYPAKIPPLPLFYQPPLWTRPKLKNSSKVLPDNALLHLGEMLRFPQEE
                     ALYPGLLQVKDACTADSLAEFAWDLFTAWLTTGAPSKESWAFTALGVLGNDDTARKLT
                     PLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQERAKEKIADIA
                     ESRELTVEELEDRLAPDLSLDDNGSLLLDFGPRQFTVSFDETLKPFVRDVSGSRLKDL
                     PKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLESAMCLRRRWSPENFQLFLVV
                     HPLVRHLTRRLIWGVYSAENQLQACFRVAEDNSYSTADDDLFTLPEGDISIGIPHVLE
                     ISPTDAAAFGQLFADYELLPPFRQLHRNSYALTEAERNASELTRWAGRKCPSGRVMGL
                     ANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSAEQLLSKLWLWE
                     GKAESYGWGSNSTQEAQLSVLDAITASELINDIEALFE"
     gene            10890..14522
                     /gene="yehI"
                     /locus_tag="ACHY03_003003"
     CDS             10890..14522
                     /gene="yehI"
                     /locus_tag="ACHY03_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFS3906205.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATDVVMARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGISKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARVVLKSYLSALPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQEWLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLM
                     LAFLHRPKETFPLILNFGATELALPVARVWRRFAVQRDLARQWILHWPEHTATALIPL
                     VFTKSSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEHLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVIGLNILTLMNNDMALIQLHHVSQRAKSSSLRENAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALIFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVNFH"
     gene            14583..14903
                     /gene="yehK"
                     /locus_tag="ACHY03_003004"
     CDS             14583..14903
                     /gene="yehK"
                     /locus_tag="ACHY03_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFS3906206.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFIQ
                     QIEK"
BASE COUNT         4110 a         3830 c         3715 g         3743 t
ORIGIN      
        1 aaaagccgaa atcatttata taaatgattt cggctttcta aatgcaatta aaatcaggtt
       61 gccaaattaa tcgtagcgat ttaacacgat tcgacgtttg ctatcactac ctgcaacaac
      121 cacttctgtc gttacacctt tggcgggcga agaaacctga tgacgggtat cgacattata
      181 cgttaactga ccacaatgct gttgaggttg actattaacc atagccatac tgcatgttgg
      241 gggactgatc ctgccattaa tcgttaatgt ggcagtctct gaagagaacg cgggagaagc
      301 caatccatag gcaagaaaaa taattcctga cagccaatac ttattcattg aacgttatcc
      361 ctgtagtaaa ggttatgcct ggatagaatg agtgcataac aaactatagc tgtacatcca
      421 ctacacagcc acgaaggatg ataatgaagc attgcctgta cgatcaatcg actttgtaga
      481 gtttcggacg aaggtccgca gaatattcgc agtattaaat aagtgttcat taatgcggca
      541 aaaacaaata caccgcatca atgtaacatc tctgcggtgt atgaagaata accagaatgg
      601 ttaataggcg ttagaccgcg cggaaggaaa tctcgcctgg aatgacttca ccctgccagt
      661 agagctgagc tgcaacgcgg tcagccagct ggcggtagat agcggtaaat tcgctctccg
      721 gacggctgat aaccgttggc gtgcctttat cgagatcctc acgtaaggaa atatgtaacg
      781 gcatctgccc cagcagttgt gtgtggtatt tctctgccag tttctctgca ccaccggtac
      841 caaaaatcgg ttcatggtga ccacagttac tgcaaatatg cacactcatg ttctcgacaa
      901 taccgagcac cggaacctcg actttctcga acatcacaat gccttttttc gcatcgatca
      961 acgcgatgtc ctgcggggtg gtgaccacga ctgcaccagt tactggaatg ttttgcgcca
     1021 gcgtcaattg aatgtcaccg gtgcctggcg gcatatcaag caccagatag tcgagatccg
     1081 gccacaaggt ttcctgcaac atctgcatta acgccttgct cgccatcggt ccacgccaca
     1141 ccatcgcatt gtcgtcagtg accagataac caattgaatt ggttgccagg ccatgagaca
     1201 taatcggtgc catatgggta ccgtccggcg aagttggacg ctgattttct gcgcccagca
     1261 tggtgggaat tgatgggccg tagatatcgg cgtccagaat gccgactttc gccccttccg
     1321 ccgccagtgc cagcgccagg tttaccgccg tggaggattt acccacgccg cctttaccgg
     1381 aactgaccgc aatgatgttc ttcacgccat taatacccgg ctgattcttc acgcgtttta
     1441 aggtggcgat attgtgcgac agtttccagt cgatagcttt cgccccggtg atacgcagca
     1501 gttcggcgct acattgctct tttaactctt caaacgcgct atgccagacg aatggcataa
     1561 ccagttccac atgcaacgtg tcgtccatcc aggcgacatg gtgcaacgct ttcagcgtgg
     1621 tgaggttatg cttcagggtt gggtgctgaa aattggccag tgtcccggcg accattgcgc
     1681 gcaaggcttc tggtgatttg gcctgggatt gttcgttcat cccgactcct tttttgtata
     1741 gataaaccat cagctgatag tttacctgaa gaatatagag aagtacttac ttaacatttt
     1801 cccatttggt actatctaac cccttttcac tattaagaag taatgcctac tatgactcaa
     1861 gtcgcgaaga aaattctggt gacgtgcgca ctgccgtacg ctaacggctc aatccacctc
     1921 ggccatatgc tggagcacat ccaggctgat gtctgggtcc gttaccagcg aatgcgcggc
     1981 cacgaggtca acttcatctg cgccgacgat gcccacggta caccgatcat gctgaaagct
     2041 cagcagcttg gtatcacccc ggagcagatg attggcgaaa tgagtcagga gcatcagact
     2101 gatttcgcag gctttaacat cagctatgac aactatcact cgacgcacag cgaagagaac
     2161 cgccagttgt ctgagcttat ctatactcgc ttgaaagaaa acggttttat taaaaaccgc
     2221 accatctctc agctgtacga tccggaaaaa ggcatgttcc tgccggaccg ttttgtgaaa
     2281 ggcacctgcc cgaaatgtaa atccccggat caatacggcg ataactgcga agtctgcggc
     2341 gcgacctaca gcccgactga attgatcgag ccgaaatcgg tggtttctgg cgctaccccg
     2401 gtaatgcgtg attctgaaca cttcttcttt gatctgccct ctttcagcga aatgttgcag
     2461 gcatggaccc gcagcggtgc gttgcaggag caggtggcaa ataaaatgca ggagtggttt
     2521 gaatctggcc tgcaacagtg ggatatctcc cgcgacgcgc cgtacttcgg ttttgaaatt
     2581 ccgaacgcgc cgggtaaata tttctacgtc tggctagatg cgccgattgg ctatatgggt
     2641 tctttcaaga atctgtgcga caagcgcggc gacagcgtaa gcttcgatga atactggaag
     2701 aaagactcca ccgccgagct gtaccacttc atcggtaaag atattgttta cttccacagt
     2761 ctgttctggc cagcaatgct ggaaggcagc aacttccgca agccgaccaa cctgtttgtt
     2821 catggctatg tgacggtgaa cggcgcgaag atgtctaagt ctcgcggtac ctttattaaa
     2881 gccagcacct ggctgaatca ttttgacgcc gacagcctgc gttactacta cactgcgaaa
     2941 ctctcttcgc gcattgatga tatcgatctc aacctggaag atttcgttca gcgcgtgaat
     3001 gccgatatcg ttaacaaagt ggttaacctg gcctctcgta atgcgggctt tatcaacaag
     3061 cgttttgacg gcgtgctggc aagcgaactg gctgacccgc agttgtacaa aaccttcact
     3121 gatgccgctg aagtgattgg tgaagcgtgg gaaagccgcg aatttggtaa agcagtgcgc
     3181 gaaatcatgg cgctggctga tctggctaac cgctatgtcg atgaacaggc tccgtgggtg
     3241 gtggcaaaac aggaaggccg cgatgctgac ctgcaggcga tttgctcaat gggcatcaac
     3301 ctgttccgcg tgctgatgac ttacctgaag ccggtactgc cgaaactgac cgagcgcgca
     3361 gaagcatttc tcaatacaga actgacctgg gatggtatcc agcaaccgct gctgggccac
     3421 aaagtgaatc cgttcaaggc gctgtataac cgcatcgata tgaagcaggt tgaagcactg
     3481 gtggaagcgt ctaaagaaga agtgaaagca gctgccgcgc cagttactgg cccactggca
     3541 gatgatccga ttcaggaaac catcaccttt gacgacttcg ccaaagttga cctgcgcgtg
     3601 gcgctgattg aaaacgcaga gtttgttgaa ggttctgaca aactgctacg cctgacgctg
     3661 gatctcggcg gcgaaaagcg taatgtcttc tccggcattc gttctgctta cccggatccg
     3721 caggcactga tcgggcgtca caccattatg gtggccaacc tggcgccacg taaaatgcgc
     3781 tttggtatct ccgaaggcat ggtaatggct gctggtcctg gcgggaaaga tattttcctg
     3841 ttaagcccgg atgctggtgc taaaccaggc catcaggtga aataatcccc cttcaaggcg
     3901 ctgcatcgac agcgcctttt ctttataaat tcctaaagtt gttttcttgc gattttgtct
     3961 ctctctaacc cgcataaata ctggtagcat ctgcattcaa ctggataaaa ttacagggat
     4021 gcagaatgag acactttatc tatcaggacg aaaaatcaca taaattctgg gcggttgagc
     4081 aacaggataa tgagcttcat attagatggg gtaaagtcgg tactaacggg caaagtcaga
     4141 taaaaagttt ttcagatgct gctgcagcgg caaaagcgga gcttaagctg atcgcagaga
     4201 aaacgaaaaa aggctatgtg gaaaatgctt cagcaaacgt gcatatctcc cccatcacca
     4261 aagccactcc tgaagttgaa acttcccctg agagtaaaaa ccaacgcccg tggctggctg
     4321 atgatgcagt tattccggta actgacgata ttaaccgatt tgcttttcct catcgctctc
     4381 gccccagaga gatcaattat ctgcgcaaag acggtgagat atggaagcgt attgcagata
     4441 acaccagggc atatgatccc gacaacaatt accgagctta tccagaaaac tggcagcagg
     4501 cttttgctga gttacaaatg cgaattctgg gtaatcaaca gacagggagt gtgcaatctg
     4561 atgcagcatt gctctggagt ttttggaact cttactctgc agatgaactg gtcgatgact
     4621 tagtcattcg ctgtggtctg gaaagcgccg ttgaaatcgt cctttttgcc cttcaactca
     4681 aatataaacc agtaaaaggc gcagtaacca ccatcattcc acctaatcac gaagcagaat
     4741 cgctacctag ttggcatcaa cgtctatgtc atcatctttc actcgcttca gaagatgagt
     4801 ggcaacgctg tgtagataaa gtactcaccg ccattccgac gctatccccc gcacggcaac
     4861 cttttgcggc gttactgctc cctgaacgcc cggatatcgc ccatgctata gctctacgct
     4921 atgcagacca aaacgtcccg gcgatgacct ggttaagact ggtgataacc gatgatactg
     4981 ctctgagtac agtggaaaga tacgacttcc caccgttgta tcgtgatttt cgtaattttc
     5041 gcgcttatct ggcaatgttg ttggcaaata atggggtgcg cggtgtaagc cggatacttc
     5101 ttgagtttac ggaagaccat tctgacaatc ccacttatct cttcgaacgc atcagcgaga
     5161 ctgaaaatct tgtcaaatgg ctatggaaaa cgaatcaccc ggatgcgatt caaattctga
     5221 tcctcggtgt aaatggcaag aaaaagcacc tggaatactt aagcaaagcc tgccaaaaac
     5281 atcctgctgc ggctattgcc gcttatgcta ctttgctggc aatacatgta aataaagagt
     5341 ggcgtaaagc gcttgtcaaa ctgattaccg ccacgccaga gttagtatgc gacgtcattc
     5401 cctgggttaa tgctaaagcg gcaggcatct tatctgaatg tcgtccgcag ccagtagctg
     5461 aagaatgtga atatgccact gtggatatgt tgccggagct gcttgtttcg ccgccctgga
     5521 tgaccaaaga gaaaaagaaa aatacgcctg tgtttgatct tccagtgctt ccagttcctt
     5581 ctgtttctga tgtcactcca gagattacca aaaaactgac ccgtacttac cttgtcaccc
     5641 acttccagca aatagcccaa cagcaggcga caaaacaaac gttgtttacc gacctgccgc
     5701 cgatcaaaaa agcaagctgg gagaaacact taataccgct tacaccggag caacaaatac
     5761 tgtggcatct tggttttgaa aaatggcggg aaagcggaga aaaaatatac gaaaaaattc
     5821 cggcgccaca aagcgccgtt gatgccctgc tacgcttcga ttttccagca ctcaatgctg
     5881 aatttgtgca atatcataac aatgcataca aaagctggaa tctcattgcc ctctgttacc
     5941 tgcctggtca acaggccatt tcttttctta accagatagt caaagaagat aactactctg
     6001 gtgagggaaa tattctggca atttttggta gcgccgccat acctgcattt atggcctgcc
     6061 tgcaacgtga tccacgacgt ctgtgcttct tccccttttt cctcggtgtc agtgaactgg
     6121 ccttaccgat ggcgcaacaa ttacagaaaa aaatgtctta tgaagatgcg cgaaactggc
     6181 taactgatta tccacgccat gccgccgccg ggctacttcc tgtggccctg ggcaaaaaag
     6241 gaaaagatcg tgattgtgcc cgccaggcgc ttcgtctgct ggtgaattta aaccagcgag
     6301 aaacgattga ggaaatcgcg caaggatata atcaacctga tgttttagct gcgctggcaa
     6361 cattgttcga tagcgaccca ctggaagaat atcccgctaa aatcgcccca ctgtccggct
     6421 tttatcagtt caccttgtgg cgcagaccac ggcttaaaag taacaacctg cctctgtcag
     6481 atgacgctat gcgccacctc ggcactatgc tgagctttcc tcgcgacatt accgcctacg
     6541 ctgggctgga tatcattaga gaaatcttca cccgcgaatc actggctgaa tttggctggg
     6601 atctgtatac cgcctggaca gaagctggcg cacccgcaaa agaaaactgg gcatttactt
     6661 cgcttgggat tttaggcaat gacgacaccg cgcgtaaatt aaccccgctt atccgcgcct
     6721 ggcctgggga atcccagcat aaacgggcgg tgtccgggct ggatgtatta gctgatattg
     6781 gcagcgatgt tgcgctaatg ctgcttaatg gcatcgcgcg aaaaattaaa ttcaaagcat
     6841 tacaggaaca tgcccgcgag aaaatcaaca tagttgctga aaaccgtggg ctgactatgg
     6901 ctgaaatgga agaccgcctg gctccagatt tagggcttga tagcagtggc tcgctgatac
     6961 tggatttcgg cccacgtcaa tttaccgttg gttttgacga aactttaaaa cctgtagtgc
     7021 gcgacgcaaa cggcaaagtc ctgaaagatt tgcccaaacc aaaccagagc gatgaaaaaa
     7081 ctcaggcaac tgacgcggtt aatctcttca aacagttgaa aaaagatgta cgcgccatag
     7141 ccagccagca gattgatcgt ctggaacagg ctatgtgcca gcgcagacgc tggacggcag
     7201 agcagttccg cctgtttctg gtggagcatc cgctggtacg tcacttaacc cgacggctgt
     7261 tatggggggt ttataacgat gaaaacgccc ttatcacctg ttttcgcgtg gcagaagaca
     7321 gcacttacag cgatgcgcag gatgagttat tcacgctgcc agcaggaaac atcggtatac
     7381 cgcatgtgct ggaaatcccc cctgaatccg ccgctgcatt caggcaaatt tacgttgatt
     7441 acgaactgct tccccctttc caacagctcg aacgtggtag ttaccacctt gctgataacg
     7501 aacgtaatgt ccatgaattg tcacgctgga acggacgtct ctgccaggcc ggacgcattg
     7561 tcgggcttga acgcagaggc tggcaacgtc tggaagaaag cggcagcgtc tatgcgatgc
     7621 gcaaatctac cccttatggt gctctcgaac ttgaaacaga acctttttca ttaatttatg
     7681 gcgaaacggg gtatagcgac ctgctgcctg tcgaaagcgt aaaaatcacc gccccttatg
     7741 atcgctatgg taaacaatcc tcacccactt tctccgtgct cgacgacatc accgccagcg
     7801 agctgattaa cgacattgaa tcactgtttg attaaaagga tttagcaatg aaaacctata
     7861 tttatcagga cgaaaaatca cataaattct gggcggtgga gcagcagaga aacgagctgc
     7921 atatcaactg gggcaaagtc ggcactaacg ggcaaagtca ggtaaaaagc tttgctgatg
     7981 ctgcggcggc agaaaaagcg gaactgaagc tgattgcgga gaaggtgaag aaggggtatg
     8041 tggagcaagt agacgctaac gctcactcac atgatgtctc agcaaagaaa gtcctggtca
     8101 cagaggaaaa agcctcaata aatcaccagg cagcaactaa tggtttaccc tggctggcag
     8161 acaacgatcc cattattctt cccccgaata ttgcccgcca tgccctcagc caccgccttt
     8221 ggccgggaga gccagtaaaa aaaccaacca aacccgagaa acaaaattta atttcccgat
     8281 tggcaaatcg tacttatcat tattataaaa atctgacttt cgattacagc tcatgctctg
     8341 atgaatgggc tgatgcgatt agccaggcaa tcagcttaat taaggcccaa aagactgaga
     8401 tacccgcgcg tactatggcg gtacttactt ttctggaaat atgtttttgt cagggctgga
     8461 atgatgaaac ctcatccgag ttgctggatg aaattgtaag caccttcggt atcgaatacg
     8521 caactgaaac tgtcatcttc tggtggcaaa ttgatttcga ttttgactat aaagatgaac
     8581 atttgacttt ctttattaat tatgcagata gcagcaagga cagttataga aggaataacg
     8641 acaaatttag tttacgtctg cgtaaacatt tatcactagc agaagaagaa gtatggcaaa
     8701 attgcattgc aaaattactc gttgcgttac cagatatatc actctatcgc caaccgttaa
     8761 ttgccatttt aatgccagaa atacccgaag tggcccatga gatcgtacat cgtttacacc
     8821 aggtcgcaga tgttccacaa cttgaaatgt tgaaactggt agcgacagat ccattcacgc
     8881 tcgaaatact ggagaactat caatacattg atgtatttaa cacctatggt gccagttggt
     8941 cagctacggt attacgggaa caaggtatag cggcactggc tcgcctggct tcctatgctg
     9001 aagaagataa ttgcggtgac gtactgaaat gtattaatca tccgttggcc attacggcac
     9061 tcatcgaagc cgccgatact aataaacgct gttatgaacg actaataaaa agtgccgaaa
     9121 attttccttc tgccacgata gcggcattag ccgaagcatt ggtaaaaaaa gatgataaac
     9181 gcaaacatac gttgctggtt tcattactta atgcaaatcc tttattggtc gaaaaattag
     9241 tcatcggatt atcgtcgcaa gcggcaaatt tattgcaaca atgtcagcaa ttgctgaata
     9301 gtcgcaatga agattttgca aactcagaaa ttctaccaat ggtactagtc tcgccgccct
     9361 gggaaactca aaataaaaat aacgacaaca gtcctgtggg taaatacccg gcgaaaatcc
     9421 cccctttacc gttgttttat caacctccat tatggactcg cccaaaactc aaaaatagca
     9481 gcaaggtact gccagataac gccctcctcc acctcggtga aatgctccgc ttccctcagg
     9541 aagaggcgct gtatccggga ttattgcagg tgaaagatgc ctgtaccgcc gactcactgg
     9601 ctgaatttgc ctgggatctg tttaccgcct ggctgactac gggtgcacca tcgaaagaaa
     9661 gctgggcgtt cactgcgtta ggtgttctcg gtaacgacga caccgctcgc aaactgacgc
     9721 cattaatacg cgcctggcct ggcgaatctc agcataaacg cgccaccgtt gggttggata
     9781 ttctcgccgc tatcggtagt gatatcgctc tgatgcagct taacggcatt gctcagaaac
     9841 tgaaattcaa agctttacag gagcgggcga aagagaaaat tgccgacatt gctgaaagtc
     9901 gcgaactgac agtggaggag cttgaagatc ggttagcacc ggatctcagt ctggatgata
     9961 acggttcgtt gctgctggat ttcggcccac gccagttcac cgtcagcttt gatgaaacct
    10021 taaaaccgtt tgtgcgtgat gtttctggca gccgtctgaa agacctgccc aaaccaaaca
    10081 aaagcgatga tgaatcacag gcgaacgatg ctgttaaccg ctacaaactg ctgaaaaaag
    10141 atgcgcgtac cgtcgccgcc cagcaggtgg caaggcttga atccgccatg tgcctgcgcc
    10201 gccgctggtc gccggaaaac ttccaactct tcctggttgt gcatccgctg gttcgccact
    10261 taacccgccg tctgatttgg ggcgtttata gcgccgaaaa ccagctgcag gcttgctttc
    10321 gcgtagcgga agataacagc tacagcaccg ctgacgatga tcttttcacc ctgccggaag
    10381 gcgatatctc tatcggcatt cctcacgttc tggaaatatc accaacggat gctgccgcct
    10441 ttggtcagct ttttgccgac tacgaactgc taccaccgtt ccgtcagctt caccgtaaca
    10501 gttacgccct gacagaagcc gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa
    10561 aatgcccgag cggtcgggta atggggctgg cgaataaagg ctggataaag ggcgaaccac
    10621 aggatggagg ctggatcggc tggatgatca aacctctggg ccgctggtcg ttaatcatgg
    10681 aaatcgatga aggctttgcg gtaggcatgt cgccagccga actgagtgcc gagcaactct
    10741 taagcaagct gtggctatgg gaaggcaaag cggaaagcta tggctggggg agtaattcaa
    10801 cacaggaagc gcagctctcc gtactcgatg ccatcaccgc cagcgagcta attaacgata
    10861 ttgaagccct gtttgaataa ggaaagagca tggacaagga attaccgtgg ctggctgata
    10921 acgcccaact ggaactgaaa tataaaaagg gtaaaacacc actcagccat cgcaattggc
    10981 cgggcgaacc agtgcctgtt atcactgaaa gcatcattca gacactgggt gatgaattgc
    11041 tacaaaaagc tgagaagaaa aaaaacattg tctggcgtta tgaaaatttt tcactggagt
    11101 ggcagtccgc catcacgcag gccatcaact tgatcggcga acacaaaccc tcaatcccgg
    11161 cccggacaat ggcggcgcta gcctgtatcg cgcaaaatga cagccaacag ttgctcgacg
    11221 aaatcgtcca acaagagggg ctggaatatg cgacggatgt agtgatggcg cgtcagtgca
    11281 ttgcgcggcg ttatgagagt gactcactgg tcgtcacttt gcagtatcag gacgaggact
    11341 atggctacgg ttatggctcc gcgacctata acgatttcga tctccgtttg cgtaagcatc
    11401 tgtcgctggc agaggaaagt tgctggcagc gttgtgccga taaactcatt gccgcactgc
    11461 caggaatatc caaaattcgc cgtcctttta ttgcgctgat cctcccggaa aaacctgaaa
    11521 tagccaatga actcgccagt cttgaaagct cccggtcaag tctccattca aaggagtggt
    11581 taaaggtcgt tgctactgat aatacggcgg taaaaaaact cgaacgatac tggggcctgg
    11641 acgtatttag cgatcgcgaa gccagctaca tgtcacaaga aaaccgcttc ggctacgcgg
    11701 cctgcgcctc ccttttgcgc gaacaaggac tggcagccgt tccgcgcctg gcaatgtatg
    11761 ctcataaaga agattgcggc agtctgctgg tacaaattaa ccatccgcaa gtcatccgca
    11821 ccttgctgtt agttgctgat aaaaacaaac ccagcctgca acgtgtggct aaatacagta
    11881 aaaacttccc ccatgcgacg ctcgccgcac tggcagaact gctggcgtta aaagaaccac
    11941 cagcccgccc tggttatcca atcatcgaag acaaaaagct gcctgcacag caaaaagctc
    12001 gagatgaata ctggcgtacg ctgttacaaa cgctgatggc atcgcagcca caactggcag
    12061 cagaagtgat gccgtggtta agtactcaag ccagggtagt gctgaagagt tatttatcgg
    12121 cactgccaaa accggttatt gatggcaccg ataacagcaa tctgcctgaa atcctcgttt
    12181 caccaccgtg gcgtagtaag aaaaaaatga cagctccacg tcttgatttg gcaccgctcg
    12241 aattaacccc gcaagtttac tggcaaccag gcgaacaaga gtggcttgcc tccactgagt
    12301 ctgcccgtta tttcagcacg gaatctcttg cggaacgcat ggaacaaaaa agtgggcgag
    12361 ttgtattaca ggaactgggt tttggggatg atgtatggct gtttctgaat tatatactcc
    12421 ccggaaaact ggatgctgca cgcaattcac tcattgttca gtggcattac taccaggggc
    12481 gggttgaaga gatcctgaat ggctggaact ccccggaagc acaattagca gaacaggcgc
    12541 tccgcagcgg tcacatagaa gcgttaatta acatatggga aaatgacaac tactcacgtt
    12601 atcgtccgga aaagagtgtc tggaacctgt atttattggc acagttgccg cgtgagatgg
    12661 ctttgacctt ctggctgcgt atcaatgaga aaaaacatct gttcgcgggt gaggactatt
    12721 ttctcagtat cctcgggttg gatgcgctac caggtctgat gttggctttt ttacatcgtc
    12781 caaaagaaac atttccgtta attttaaatt ttggcgcaac agaactggcc ctgcccgttg
    12841 cccgcgtctg gcgacgtttc gcggtgcagc gtgatctggc tcgccagtgg attttacatt
    12901 ggccggaaca tacggctact gcacttatcc ctcttgtctt caccaaatcc agcgataata
    12961 gcgaagctgc attacttgcc ctgcgtttac tttacgaaca gggacatggt gaattgctgc
    13021 aaaccgtggc aaaccgctgg cagcgtacag atgtatggcc tgccctggag catttactga
    13081 aacagggtcc aatggacatt tacccggcac gcattccaaa agcccctgat ttctggcatc
    13141 cggcaatgtg gtccaggccg cgacttatca ctaataatca gcctgttacc gatgacgctc
    13201 tggaaattat cggcgaaatg ctgcgcttta cccagggcgg acgtttttat agcggtctgg
    13261 aacaactgaa aacgttttgc cagccacaaa cgctggccgc ttttgcctgg gatctcttca
    13321 ctgcgtggca acaagctggt gcccccgcaa aagacaactg ggcatttctg gcgttaagtc
    13381 tctttggtga cgaaagcacg gctcgggatc tgacgacaca gatcctcgcc tggccacaag
    13441 aaggcaaatc tgcccgtgct gtcattggtc tgaatatact taccttgatg aataatgata
    13501 tggcgctgat acagctgcat catgtatcac aacgggcgaa atcctcttca ttacgtgaaa
    13561 acgcagcgga atttcttcag gtggtcgcag aaaatcgcgg gctaagccag gaagagttag
    13621 cggacagatt agtcccaaca ctgggtcttg atgatccgca ggcgttgatt tttgattttg
    13681 gtccccggca gtttaccgtt cgcttcgatg aaaacctcaa cccggttatc tttgatcaac
    13741 aaaacgttcg ccagaaaagt gttccccggt tgcgtgccga tgacgatcaa ctgaaagcgc
    13801 ccgaggcact ggcccgacta aaagggctaa aaaaagatgc tactcaggtg agcaaaaacc
    13861 tgctcccgcg tcttgaagct gccctacgta ccacccgacg ctggtcgctg gcagattttc
    13921 attctctgtt tgttaatcat ccctttaccc gcctggttac ccagcgctta atatgggggg
    13981 tttatccggc aaatgaaccg cgtcgtttac tcaacgcctt tcgtgtggcc gcagaggggg
    14041 agttctgcaa tgcgcaggat gagcccattg accttcctgc ggatgctctg attggcattg
    14101 cccacccgtt agaaatgaca gcagaaatgc gcagtgaatt tgcacagctt tttgccgatt
    14161 acgaaattat gccgcctttt cgccagttgt cgcgccgcac ggtgctgctc acacctgacg
    14221 agtcaaccag taacagcctg actcgctggg aaggtaaatc cgctaccgtt gggcaactta
    14281 tgggaatgcg atacaaaggc tgggagtcag gctatgagga cgcatttgtc tatgacctgg
    14341 gtgagtaccg gctggtcctt aagttttcac ccggttttaa ccactacaat gttgatagca
    14401 aagcgctaat gagcttccgt tctcttcgag tgtaccgtga caataaatcc gtcacttttg
    14461 ccgaacttga tgtgtttgat ttgagtgagg cgttaagcgc acctgacgtc aatttccatt
    14521 aaaacacagc gggcagtgat gcaactgccc gttattaaca cgacttacca gcggggaaag
    14581 cgatgatcgt gcaaaaagag ctggttgcta tttacgatta tgaggtccct gtacctgaag
    14641 atccgttttc cttcagactt gagatccata aatgctctga attatttaca ggttccgtct
    14701 atcgactgga gcgattccgg ctacatccaa catttcatca acgtgatcga gaagatgctg
    14761 acccgctaat aaatgatgcg ttgatttata taagagatga gtgtattgat gagcggaaat
    14821 tacgaggtga atcacctgaa actgtaatag caatttttaa tcgtgaacta cagaatatat
    14881 tcatccaaca aatagaaaaa tagctttatt aaagggggaa acggtattcc cccttagatg
    14941 cgtttcaatt tgcccgttac ttcaacaaat caatcaaccg taacagagcc actaataact
    15001 ccagtacggc aatgagcacg gttaactttt gtgtcagcgt gtcaatccgc tctccttatt
    15061 aaggagcgca acaccagcac cttaccctta cactgcctgt cgatactggt ttacgtatgt
    15121 tggtacgaaa cgttgctctc cggatcaggg caccgcctgc ggcaccaccc gtccggcagc
    15181 caggacttaa aattggtccg atccggcaca acctcccaac gggcaggatt ataaatctca
    15241 ctcactgaat tgcaatggag tgtggatatg gttataattt attcgttggt acgaaatgtt
    15301 gcacaatcag caagttagcg tcactatagt aaataaaaga acctgcctca ccagcaggtt
    15361 tttttatttt ctgcgatcta ctttccagat attttttg
//
LOCUS       DAZUCE010000114        15340 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZUCE010000114 DAZUCE010000000
VERSION     DAZUCE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.114"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            206..541
                     /locus_tag="ACHY03_003005"
     CDS             206..541
                     /locus_tag="ACHY03_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFS3906207.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            901..1344
                     /gene="yjdN"
                     /locus_tag="ACHY03_003006"
     CDS             901..1344
                     /gene="yjdN"
                     /locus_tag="ACHY03_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFS3906208.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AEGCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1477..2265
                     /gene="phnC"
                     /locus_tag="ACHY03_003007"
     CDS             1477..2265
                     /gene="phnC"
                     /locus_tag="ACHY03_003007"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFS3906209.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLIVGDKKPECRVELLGRTVQQNGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2290..3306
                     /gene="phnD"
                     /locus_tag="ACHY03_003008"
     CDS             2290..3306
                     /gene="phnD"
                     /locus_tag="ACHY03_003008"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3906210.1"
                     /translation="MNAKIIASLAFTSMFSLSTLFSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVEGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3412..4191
                     /gene="phnE"
                     /locus_tag="ACHY03_003009"
     CDS             3412..4191
                     /gene="phnE"
                     /locus_tag="ACHY03_003009"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFS3906211.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4212..4937
                     /gene="phnF"
                     /locus_tag="ACHY03_003010"
     CDS             4212..4937
                     /gene="phnF"
                     /locus_tag="ACHY03_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFS3906212.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGIAEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4938..5390
                     /gene="phnG"
                     /locus_tag="ACHY03_003011"
     CDS             4938..5390
                     /gene="phnG"
                     /locus_tag="ACHY03_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFS3906213.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQTRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5387..5971
                     /gene="phnH"
                     /locus_tag="ACHY03_003012"
     CDS             5387..5971
                     /gene="phnH"
                     /locus_tag="ACHY03_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFS3906214.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGAAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5971..7035
                     /gene="phnI"
                     /locus_tag="ACHY03_003013"
     CDS             5971..7035
                     /gene="phnI"
                     /locus_tag="ACHY03_003013"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFS3906215.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLIRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPYFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            7028..7873
                     /gene="phnJ"
                     /locus_tag="ACHY03_003014"
     CDS             7028..7873
                     /gene="phnJ"
                     /locus_tag="ACHY03_003014"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFS3906216.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGENDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7870..8628
                     /gene="phnK"
                     /locus_tag="ACHY03_003015"
     CDS             7870..8628
                     /gene="phnK"
                     /locus_tag="ACHY03_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFS3906217.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQLGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8739..9419
                     /gene="phnL"
                     /locus_tag="ACHY03_003016"
     CDS             8739..9419
                     /gene="phnL"
                     /locus_tag="ACHY03_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFS3906218.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9416..10552
                     /gene="phnM"
                     /locus_tag="ACHY03_003017"
     CDS             9416..10552
                     /gene="phnM"
                     /locus_tag="ACHY03_003017"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFS3906219.1"
                     /translation="MIINNVKLVLENEVVSGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNSFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10552..11109
                     /gene="phnN"
                     /locus_tag="ACHY03_003018"
     CDS             10552..11109
                     /gene="phnN"
                     /locus_tag="ACHY03_003018"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFS3906220.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRLREQAQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQQLFALSWHANGYYYGVGLEIDLWLHAGFDVVVNGSRAHL
                     PQARARYASALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            11096..11530
                     /gene="phnO"
                     /locus_tag="ACHY03_003019"
     CDS             11096..11530
                     /gene="phnO"
                     /locus_tag="ACHY03_003019"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFS3906221.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11532..12290
                     /gene="phnP"
                     /locus_tag="ACHY03_003020"
     CDS             11532..12290
                     /gene="phnP"
                     /locus_tag="ACHY03_003020"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFS3906222.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPCEDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12367..12696)
                     /gene="yjdP"
                     /locus_tag="ACHY03_003021"
     CDS             complement(12367..12696)
                     /gene="yjdP"
                     /locus_tag="ACHY03_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFS3906223.1"
                     /translation="MKRFPLFLLFTLLTLTTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12765..13082)
                     /locus_tag="ACHY03_003022"
     CDS             complement(12765..13082)
                     /locus_tag="ACHY03_003022"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFS3906224.1"
                     /translation="MQPVFFIKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEGIDKET
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13335..15320
                     /gene="yjcS"
                     /locus_tag="ACHY03_003023"
     CDS             13335..15320
                     /gene="yjcS"
                     /locus_tag="ACHY03_003023"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFS3906225.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSIRLDSEKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3432 a         4567 c         4271 g         3070 t
ORIGIN      
        1 aaggagagca ttaaccttat cttaaataat aaatgaaggc aaaaagatta ttttcttttt
       61 gcgtttcctt tcaaatgaaa acgatcgtcg tctaaaatca gcagtacccc cgacaaactc
      121 agggattttg tgtataattg cggccttttt cggcaatctg ccgttttttg gcgcttttgc
      181 cctgctgact tttgaggaaa tccacatgtc attaccacac tgcccaaaat gcaactccga
      241 atacacctac gaagataacg gcatgtacat ctgcccggaa tgtgcctacg aatggaacga
      301 cgcagaacct gcacaggaaa gcgacgagct gatcgttaaa gatgctaacg gcaatctgct
      361 ggctgacggc gacagcgtta ccatcattaa agatctgaag gtgaaaggta gctcttcgat
      421 gctgaaaatt ggcaccaaag tgaaaaacat ccgcctggtt gaaggcgacc ataacatcga
      481 ttgcaaaatc gacggttttg gtccgatgaa actgaaatct gagtttgtga aaaagaactg
      541 attgtattgt gatcggtagg ccggataagg cgtttacgcc gcatccggca acggtgccag
      601 atgcctgatg cgacgcttgc gcgtcttatc aggcctacaa attcccgcac cctccgtagg
      661 ccggataagg cgtttacgcc gcatccggca acagtgccag atgcctgatg cgacgctcgc
      721 gcgtcttatc aggcctacaa attcccgcac cctccgtagg ccggataagg cgtttacgcc
      781 gcatccggca acagtgccga ctgcctgatg cgacgcttac cgcgtcttat caggcctccc
      841 cctcatctgt ataaatttcg aactacactt aactggcttc tcttaactga ggtcaccatc
      901 atgccgttaa gtccctacct ctcttttgcc ggtaactgtg ccgacgcaat tgcttattat
      961 caacgtacgt tgggcgcgga actgctctat aaaatcagct tcggcgaaat gccaaaatca
     1021 gcgcaggaca gcgccgaagg ctgcccttcc ggaatgcaat ttcccgatac tgccatcgct
     1081 catgccaacg tgcgtattgc cggaagcgat atcatgatga gcgatgctat accgtcagga
     1141 aaagccagct actccggttt tacgctggtg ctcgattcgc aacaggtcga agaaggaaaa
     1201 cgctggtttg ataaccttgc agctaacgga aaaatcgaaa tggcctggca ggaaactttc
     1261 tgggcgcatg gctttggcaa agtcaccgat aaatttggcg taccgtggat gattaatgtc
     1321 gtcaaacaac aaccaacgca ataatccgcc gggaggcccg ccctcccgca ctgtcatcga
     1381 attcccgtta actcttcatc tgttagtcac ttttaattaa ccaaatcgtc acaataatcc
     1441 gccacgatgg agccactttt ttagggaggc tgcatcatgc aaacgattat ccgtgtcgag
     1501 aagctcgcca aaaccttcaa tcagcatcag gcgctgcatg cggttgatct gaacattcat
     1561 cacggtgaaa tggtggctct gcttgggccg tcgggttccg gcaaatccac ccttttacgt
     1621 cacttaagcg gcctgattgt cggcgataaa aagccagaat gccgcgtcga gctgctgggc
     1681 cgcaccgtcc agcaaaatgg ccgcttagcc cgcgatatcc gcaaaagccg cgccaacacc
     1741 ggctacatct tccaacaatt caacctggtg aatcgcctga gcgtactgga gaacgtgctg
     1801 attggcgcgc tcggcagcac gccgttctgg cgcacctgtt ttagttggtt cacccgcgag
     1861 cagaaacagc gcgcgttaca ggcgctgacc cgcgttggca tggtgcattt tgcccatcag
     1921 cgcgtttcca ccctctccgg cggacagcag cagcgtgtgg cgattgcccg cgcgctgatg
     1981 cagcaggcga aggtgattct ggcggacgaa ccgatcgcct cgctggaccc ggaatccgcc
     2041 cgcatcgtga tggacaccct gcgcgacatc aaccagaacg acggcatcac cgtggtcgtc
     2101 acgctgcatc aggtggatta cgccctgcgc tactgcgagc gcatcgtcgc cctgcgccag
     2161 gggcacgtct tctacgacgg cagcagccaa cagtttgata acgaacgttt tgaccatctc
     2221 taccgcagca ttaatcgcat cgaagagaac gcgaaagctg cctgacatcc ccatcattga
     2281 ggaaaacgaa tgaacgctaa gataattgcc tcgctggcct tcaccagcat gttcagcctc
     2341 agcaccctgt ttagcccggc gcacgccgaa gagcaggaaa aggcgctgaa tttcggcatt
     2401 atttcaacgg aatcacagca aaacctgaaa ccgcaatgga cgccgttttt acaggatatg
     2461 gagaagaagc tgggcgtgaa ggtcaacgcc ttctttgccc cggactacgc gggcattatc
     2521 cagggaatgc gcttcaacaa agtggatatc gcctggtacg gcaacctgtc ggcgatggaa
     2581 gcggtggatc gcgccaacgg tcaggtcttc gctcagaccg tcgcggcgga cggatcgccg
     2641 ggttactgga gcgtgttgat cgtcaacaaa gatagcccga tcaacaacct gaacgatctg
     2701 ctggcgaagc ggaaagatct caccttcggc aatggcgatc ctaactccac ctctggcttc
     2761 ctcgtccccg gctactacgt cttcgccaaa aacaatatct ccgccagcga cttcaagcgc
     2821 accgtcaacg ccgggcatga aaccaacgcg ctggccgtcg ccaacaagca ggtggatgtt
     2881 gccactaaca acaccgaaaa cctcgacaag ctgaaaacct ccgcgccgga gaagttgaaa
     2941 gaactgaaag tgatctggaa gtcgccgctg atcccaggcg atccgatcgt ctggcgtaaa
     3001 aacctttccg aaaccaccaa agacaagatc tacgacttct ttatgaacta cggaaaaacg
     3061 ccggaagaga aagcggtgct ggaacgcctg ggctgggcgc cgttccgcgc ttccagcgac
     3121 ctgcaactgg tgccgattcg ccagctcgca ctgtttaaag agatgcaaag cgtggaaggc
     3181 aataaagggc tgaatgagca ggacaagctg gcaaaaacca ccgagattca ggcgcagctg
     3241 gatgacctgg accgcctgaa caacgcgcta agcgcgatga gttcggtgag taaagcggtg
     3301 cagtaaatcg taggtcggat aagacgcccc ggcgtcgcat ccgacaatgt gcaggcgttg
     3361 atgccggatg cggtgcaagc accttatccg gcctacagac cggagccaaa catgcaaacc
     3421 atcaccatcg ccccacccaa gcgcagctgg ttctcgcttc tgagctgggc cgttgttctc
     3481 gccgtgctgg tcgtctcgtg gcagggcgcg gaaatggccc cgctcacgct gattaaagac
     3541 ggcggcaaca tggcgacgtt cgccgccgac ttcttcccgc ccgatttcag ccagtggcag
     3601 gattacctca ccgaaatggc cgtcacgctg caaatcgccg tctggggcac cgcgctggcg
     3661 gtggttctct ccatcccctt cggcctgatg agcgccgaaa acctggtgcc atggtgggtt
     3721 taccagcccg ttcgccgcct gatggacgcc tgccgcgcca ttaacgaaat ggtcttcgcc
     3781 atgctgttcg tggtcgccgt cggcctcggc ccgttcgctg gcgtgctggc gctgtttatc
     3841 cacaccaccg gcgtgctctc caagctgctt tccgaagcgg tggaagcgat cgaacccggc
     3901 ccggtggaag gcattcgcgc gaccggcgcc aacaagctgg aagagatcct ctacggcgtg
     3961 ctgccgcagg tgatgccgct gctgatctcc tactccctct atcgcttcga atctaacgtc
     4021 cgctcagcga ccgtcgtcgg catggtcggc gcgggcggga tcggcgtcac gctgtgggaa
     4081 gcgattcgcg gtttccagtt ccagcaaacc tgcgccctga tggtgctgat tatcgtcacg
     4141 gtcagcctgc tggatttcct ctctcaacgg ttgcgtaagc actttatctg attagcgagg
     4201 cattgatagc tatgcacttg tctacacatc cgaccagcta cccaacacgc tatcaggaga
     4261 tagccgcaaa acttgagcag gagctgcgtc aacactaccg ctgcggcgac tatcttcccg
     4321 ccgagcagca gctggcggcg cgctttgagg tgaaccggca caccctgcgc cgcgccatcg
     4381 accaactggt ggaaaaaggc tgggtacagc gccgtcaggg cgtcggcgtg ctggtgctga
     4441 tgcgcccgtt cgattacccg ctcaacgccc aggcgcgttt tagccagaat ctacttgatc
     4501 agggcagtca ccccaccagc gaaaaactgc tgtctgtgct ccgcccggcc tccggccacg
     4561 tcgctgacgc gctgggtatc gccgagggcg agaacgttat ccacctgcgc accctgcgcc
     4621 gggtcaacgg cgtcgcgctc tgtttaatcg accactactt tgcggacctc accctctggc
     4681 cgacgctgca acgcttcgac agcggctcgc tgcacgattt tctgcgcgag caaacgggga
     4741 ttgcgctgcg ccgtagccag acgcggatca gcgcccgccg cgcccaggcc aaagagtgcc
     4801 agcgtcttga aatcccgaat atgtcgccgc tgctgtgcgt gcgcaccctt aaccaccgtg
     4861 acggtgaaag cagcccggcg gagtactccg tcagcctgac gcgcgccgac atgattgaat
     4921 tcactatgga gcactgaatg cacgcagata ccgcgacccg ccagcactgg atgtccgtac
     4981 tggcgcacag ccaaccggca gaactggcag cccgcctgaa cgcgctgaac atcaccgccg
     5041 actatgaggt gatccgcgcc gctgaaactg gcctggtaca gattcaggcg cggatgggcg
     5101 gcaccggtga acgctttttt gccggcgacg ccacgctcac ccgcgccgcc gtacgcctga
     5161 ctgacggcac gctcggctac agctgggtgc tggggcgcga caaacagcac gccgaacgct
     5221 gcgcgctgat tgacgcgctg atgcagcaaa cccgccactt tcaaaactta tcagaaactc
     5281 ttattgcccc gctggacgct gaccgtatgg cacgcattgc cgcacgccag gccgaagtga
     5341 acgccagccg ggtcgacttc tttacgatgg ttcgcggaga caacgcatga ccctggaaac
     5401 cgcttttatg cttcccgtgc aggatgccca gcacagtttt cgtcgcctgt taaaggccat
     5461 gagcgagccg ggcgtgattg tcgccctgca tcagctcaaa cgcggctggc agccgctgaa
     5521 tatcgccacc accagcgtgc tgctgacgct ggccgataac gacacgccgg tgtggcttgc
     5581 cgcgccatta agtaacgata tcgtcagcca gagcctgcgt tttcatacca acgcgccgct
     5641 ggtcagccag ccggaacagg cgaccttcgc ggtaacggat gaggcgattt ccagcgaaca
     5701 gctcaacgcc ctttccaccg gcgccgccgt tgcgccggaa gcgggcgcga cgctgatttt
     5761 acaggtcgcc agcctgagcg gcgggcgcat gctgcgcctt accggcgcgg gcattgccga
     5821 agaacgaatg atcgccccgc agctgccgga gtgcattctg cacgaactca ccgagcgccc
     5881 gcacccgttc ccgctcggca tcgacctgat cctgacctgc ggcgagcgcc tgctggctat
     5941 cccgcgaacc actcatgtgg aggtgtgctg atgtacgttg ccgtgaaagg gggcgagaag
     6001 gcgatcgacg ccgcccacgc cctgcaagag agccgacgcc ggggcgatac cgatttgccc
     6061 gaactgagcg tcgcccagat tgaacagcag cttaaccttg cggtggatcg cgtgatgacc
     6121 gaaggcggca ttgccgaccg cgaactggcg gcgctggcgc tgaaacaggc cagcggcgat
     6181 aacgtcgaag caattttcct gctgcgcgcc taccgcacca cgctggcgaa gctcgcggta
     6241 agcgagccgc tcgacaccac cgggatgcgc ctcgaacggc gtatctccgc cgtttataaa
     6301 gacattcccg gcggccagct gcttggccca acctacgact acacccatcg cctgctcgat
     6361 ttcaccctgc tggcaaacgg cgaagcgccg acgctgacca ccgccgacag cgaacaacag
     6421 ccgtcgccgc acgttttcag cctgctggcg cgtcaggggc tggcgaagtt tgaagaggat
     6481 agcggcgcac agccggacga catcacccgc acgccgccgg tttacccctg ctcacgttct
     6541 tcccgtttgc agcagttgat tcgcggcgac gaaggctatt tgctggcgct ggcctactcc
     6601 acccagcgcg gttacgggcg caaccacccg ttcgcgggcg agatccgcag cggctatatc
     6661 gacgtatcga ttgtgccgga agagctggga tttgcggtaa acgtcggcga actgctgatg
     6721 accgagtgtg aaatggttaa cggttttatc gacccgccgg atgaaccgcc gtacttcacg
     6781 cgcggctacg ggctggtgtt cggcatgagc gagcgcaaag cgatggcgat ggcgctggtc
     6841 gaccgcgctc tgcaagcccc ggagtacggc gagcacgcga caggcccggc gcaggatgaa
     6901 gagttcgtgc tggcacatgc cgacaacgtc gaagccgcag gctttgtctc acacctcaaa
     6961 ctcccgcact acgtcgattt ccaggccgaa ctggagctac tcaaacgtct gcaacaggag
     7021 cagaaccatg gctaatctga gcggctacaa ctttgcctac ctcgacgagc agaccaaacg
     7081 catgatccgc cgcgccatct taaaagcggt ggcgatcccc ggttatcagg tgccgtttgg
     7141 cgggcgcgag atgccaatgc cgtacggctg gggaaccggc ggcattcagc ttaccgccag
     7201 cgtgattggc gaaaacgacg tgctgaaggt gattgaccag ggcgcggatg acaccaccaa
     7261 cgccgtgtcg attcgcaact tcttcaagcg cgtgaccggg gtaaacacca cggaacgtac
     7321 ggacgatgcg acggttatcc agacgcgtca ccgcatcccc gaaacgccgc tgaccgaaga
     7381 tcagataatt atcttccagg tgccgatccc cgagccgctg cgctttatcg agccgcgcga
     7441 aacggaaacc cgcaccatgc acgcgctgga agagtacggc gtgatgcagg tgaaactgta
     7501 tgaagatatt gcccgcttcg gtcatatcgc cactacctac gcctatccgg tgaaggtgaa
     7561 cgggcgctac gtgatggacc cgtcgccgat cccgaaattc gataacccga aaatggacat
     7621 gatgcccgcc ctgcaactgt tcggcgcggg gcgcgagaag cgcatctatg cggtgccgcc
     7681 gtttacccgc gtggaaagcc tcgatttcga cgatcacccg ttcaccgttc agcagtggga
     7741 tgaaccatgc gccatctgcg gatcgaccca cagctatctt gatgaagtgg tgctggatga
     7801 cgccggaaac cgcatgtttg tctgctccga taccgattat tgccgccaac agagcgaggc
     7861 aaaaagccaa tgaatcaacc gttactttcg gtcaataacc tgacccacct ttacgcgccg
     7921 ggcaaaggct ttagcgatgt ctcttttgat ttatggccgg gagaagtgct gggcattgtc
     7981 ggggaatccg gctccgggaa gaccacgctg ctgaaatcaa tctccgcgcg tctgacgccg
     8041 caactggggg agattcacta tgaaaaccgt tccctctatg gcatgagcga ggccgaccgc
     8101 cgtcgcctgc tgcgcaccga atggggcgta gtgcatcagc atccgctcga cggcctgcgc
     8161 cgccaggtgt cggcaggcgg caatatcggc gagcggctga tggcaaccgg ggcgcgtcat
     8221 tacggcgaca ttcgcgctac cgcgcagaag tggctggaag aggtggaaat tcccgccaac
     8281 cggatcgacg acctgccgac caccttttcc ggcggtatgc agcagcgttt gcagattgcc
     8341 cgcaacctgg tgacgcatcc gaagctggtg tttatggacg agccaaccgg tgggctggat
     8401 gtgtcggtgc aggcgcgcct gctcgacctg ctgcgcggcc tggtggtgga gctgaacctg
     8461 gcggtggtga ttgtcaccca cgatttaggc gtcgcccgcc tgctggcgga ccgtttgctg
     8521 gtgatgaagc aggggcaagt ggtggagagt gggttaaccg accgcgtgct cgacgacccg
     8581 catcatccgt atacacagct gctggtgtca tcggttttgc agaattgagc cggtgccgga
     8641 tgcggcgtaa acgccttatc cggcctacaa atgcgctccc cgtaggtcgg ataagacgcg
     8701 tcagcgtcgc atccgacacc cgaaccacga ggcgaaaaat gattaacgta caaaacgtca
     8761 gtaaaacctt catcctgcac cagcaaaacg gcgtgcgcct gcccgtcctc aaccgcgcct
     8821 cgctcatcgt caacgcgggc gaatgcgtgg tgctccacgg ccattccggc agcggaaaat
     8881 caaccctgct acgctcgctg tacgccaact atctgcccga cgaagggcaa atccagatca
     8941 aacacggtga cgagtgggta gacctggtca ccgcgccagc gcgcaaagtg gtggagatcc
     9001 gcaaaaccac cgtcggctgg gtgagccagt ttctgcgcgt catcccgcgt atttcggcgc
     9061 tggaagtggt gatgcagccg ctgctcgata ccggcgttcc gcgtgaagcc tgcgccgcta
     9121 aagccgcgcg tctactcacc cgcctgaacg tgccggaacg cctgtggcgc ctggcgccat
     9181 cgaccttctc cggcggcgaa cagcagcgcg tcaacatcgc ccgcggcttt atcgtcgact
     9241 accccattct gctgctcgac gaacctaccg cctcgctgga cgccaaaaac agcgccgcgg
     9301 tggtggaact gatccgcgaa gccaaagccc gcggcgcagc cattgtcggc atcttccatg
     9361 acgaagccgt acgtaatgac gttgccgacc gcctgcaccc aatgggagcc tcttcatgat
     9421 tatcaataac gtcaaattgg tgctggaaaa cgaggtggta agcggttcgc tggaggtaca
     9481 ggacggcgaa atccgcgcct ttgccgaaag ccagagccgc ctgccggagg cgatggacgg
     9541 cgaaggcggc tggctgctgc cggggctgat tgagctgcat accgataatc tggataaatt
     9601 cttcaccccg cgcccgaaag ttgactggcc tgcccactcg gcgatgagca gccacgacgc
     9661 gctgatggtg gcgagcggca tcaccaccgt actggatgcc gtggcgattg gcgacgtgcg
     9721 cgacggcggc gatcggctgg agaatctgga gaagatgatc aacgccatcg aagagacgca
     9781 gaaacgcggc gtcaaccgcg ccgagcaccg cctgcacctg cgctgcgagc tgccgcacca
     9841 caccacgctg ccgctgtttg aaaaactggt gcagcgcgaa ccggtgacgc tggtgtcgct
     9901 gatggaccac tcgccgggcc agcgccagtt cgccaaccgc gagaagtatc gcgagtatta
     9961 tcagggtaaa tactctctca ctgatgcgca gatgcagcag tatgaagagg aacagctggc
    10021 gcttgctgca cgctggtcgc agccaaatcg cgaatccatc gccgcaatgt gccgcgcgcg
    10081 acaaattgcg ctcgccagcc acgatgacgc cacgcacgcc cacgtggccg aatcccatca
    10141 gcttggcagc gtgatcgccg aatttcccac cacgttcgaa gcggcagaag cctcgcgcaa
    10201 gcatggtatg aacgtgctga tgggcgcgcc aaatatcgtg cgtggcggtt cgcactccgg
    10261 caacgtggcg gccagtgaac tggcgcagct tggcctgctg gatatcctct cttccgacta
    10321 ctaccccgcc agcctgctgg atgcggcgtt ccgcgtcgcc gatgacgaga gcaacagctt
    10381 tacgctgccg caggcggtga agctggtgac taaaaatcca gcgcaggcgc ttaatctcca
    10441 ggatcgcggg gtgattggcg agggcaaacg cgcggacctg gtgctggcgc atcgcaaggg
    10501 caatcacatt catatcgacc acgtctggcg tcagggtaaa agggtgttct gatgacggga
    10561 aaactgattt ggttaatggg gccgtccggc tccgggaaag atagcctgct ggcggaactc
    10621 cgcctgcggg aacaggcgca gttactggtg gctcatcgct acatcacgcg cgacgccagc
    10681 gccggaagcg aaaaccatat cgccctgagc gagcaggagt tttttacccg cgcaggtcag
    10741 caactcttcg cgttaagctg gcatgctaac ggctactatt acggcgtcgg gctggagatc
    10801 gacctctggc tgcatgccgg attcgacgtg gtggtcaacg gctcacgcgc ccatctgccg
    10861 caggcgcggg cacgctatgc atcggcgctg ctgcccatct gcttacaggt gtcgccagag
    10921 atcctgcgcc agcgcctgga aaaccgtggt cgtgaaaacg ccagtgaaat taacgcccgg
    10981 ctggcgcgcg ccgcccgcta tactcctcag gattgccata cgctcaataa tgacggcagc
    11041 ctgcgccagt cggtcgacac gctgctgacg ctgatccatc agaaggagaa acaccatgcc
    11101 tgcttgtgag cttcgcccgg ccacacagta cgacaccgac gcggtttacg cgttgatttg
    11161 tgagctaaaa caggcggagt ttgaccacca cgcgtttcgc gtgggcttta acgccaatct
    11221 gcgcgacccg aacatgcgct accatctggc tctgctggat ggcgaagttg tcggtatgat
    11281 cggcctgcat ttgcagtttc atctgcatca tgtcaactgg atcggcgaaa ttcaggagct
    11341 ggtggtgatg ccgcaggcgc gcggtctgaa cgtcggcagt aagttactgg cgtgggcaga
    11401 agaagaagcc cgccaggccg gggccgaaat gaccgagctt tcgaccaacg tgaagcgcca
    11461 cgacgcacac cgtttctatc tgcgcgaagg ctacgagcag agccacttcc gcttcacgaa
    11521 ggcgctgtaa catgagcctg accctcacgc tcaccggcac cggcggcgca cagggcgttc
    11581 cggcatgggg ctgcgagtgc gcggcctgcg ccagagcacg acgctcgccg cagtatcgcc
    11641 gccagccgtg cagcggcgta gtgaagttta acgaggcgat caccctgatc gacgccgggc
    11701 tgcacgatct caccgatcgc tggtcgccag gatcgttcca gcagtttttg ctgacgcact
    11761 accatatgga tcacgtccag gggctgtttc cgctgcgctg gggcgtgggc gatccgatcc
    11821 cggtctacgg cccgccggat gaacagggct gcgacgatct gtttaaacat ccgggcctgc
    11881 ttgatttcag ccacacggtg gaaccgtttg tggtgtttga tttgcagggg ttgcaggtca
    11941 cgcccctgcc gctcaaccac tcaaaactga ccttcggtta tctgctggaa acggcgcaca
    12001 gccgggtggc gtggctgtct gacaccgctg gcctgccgga aaaaacgctg aaatttttac
    12061 gcaataatca gccgcaggta atggtgattg attgcagcca cccgccgtgc gaggatgcac
    12121 cgcgtaatca ctgtgattta aataccgtgc ttgcgctgaa tcaggttatc cgctcgccgc
    12181 gcgtgattct gacccatatc agccaccagt ttgatgcgtg gctgatggaa aacgcactgc
    12241 cgtcggggtt tgaggcgggg tttgatggga tggagattgg ggtggcgtga tgagagggaa
    12301 tgtgcgtgct ggccccctca ccctaaccct ctccccagag gggcgatgcc tgctccatac
    12361 ccaacatcat cgcccatact catcttccat tctccgctct tcatcctcca gttgccgacg
    12421 ctcctgatca agctggcgct ggcgatcgtc cagctgcctg cggcgatctt caaactggcg
    12481 gcggcggtcg tcatattgtc tgcgccgatc gtcgctcact tcacgctgcc agccatcgtc
    12541 gcgcgaatct tcatagtctc gcccacggtc agggttataa gcatcgttaa tcgcctgctg
    12601 aatattgcca atggtgtcgt caataatatc ggcctgggcc ggtacagtgg tcagcgtgag
    12661 cagggtgaat aaaagaaata gcggaaagcg tttcattagc caacctcaaa aagaaactct
    12721 atccacatta gtcattactc atccatgcaa gtagtggatg aatctcaatt tctccgctgc
    12781 tctattgccg taatcgcctc cacgcgttgt tgatgacgac cgccttcgta ctgtgcgccc
    12841 agccacgcat ccacaatcat ttttgccagt tcgaggccaa ccactcgtga accaaaagcc
    12901 agtgcaactt cactggcgta atgcggataa tcagtgcgct ctgacgacct ggtttcttta
    12961 tcaatcccct cactcattga ccatgaataa aaggctggca cagcaacaca gaatcctgcg
    13021 gtgccagcga catgaccgcc cattgctccg gtacgttttt taataaaaaa caccggttgc
    13081 attgattaat tgtgctcagt atttgccgaa gtacaatata tataataaat atataaaaca
    13141 cctcatatta tttacagaga taataaattc gcgtactttt ccctcttcag cacaaatgcc
    13201 taataaaatg gatcgttccg ctcacacttc cacttattaa agtcgcaatt tcccaattat
    13261 gataacgcca gctttaaatg acgtattttg catggattac atatttacga cataaatgat
    13321 aaatggagat ttatatgaat aactctcggt tattccgttt gagcaggatt gttattgcgt
    13381 taactgccgc cagcggcatg atggtaaata ccgctaacgc gaaagaggaa gcgaaagccg
    13441 ccactcaata tacccaacag gttaatcaga attacgccaa atcattaccg tttagcgatc
    13501 gtcaggattt tgacgatgcc cagcgtggat ttatcgcccc gctgctggat gaaggtattc
    13561 tgcgtgatgc gaacggtaaa gtttactacc gcgcggacga ttacaaattt gatattaatg
    13621 ccgcagcgcc ggaaaccgta aaccccagcc tgtggcgtca gtcgcagatc aacggcattt
    13681 ctggcctgtt caaagtcacc gataaaatgt atcaggtgcg cggccaggat atctctaaca
    13741 ttacgttcgt tgagggcgag aaaggcatta ttgttatcga cccgctggtg acgccacctg
    13801 ccgcaaaagc cgcacttgac ctttacttcc agcatcgtcc gcaaaaaccg attgttgccg
    13861 ttatctacac tcacagccac accgaccact atggtggcgt gaaaggcatt atctctgaag
    13921 ccgatgttaa atccggcaaa gttcaggtga ttgcccctgc tggctttatg gacgaagcca
    13981 tcagcgaaaa cgtgctggcg ggtaacatca tgagccgccg tgcgctctac tcttacggtc
    14041 tgttactgcc acacaacgcg caaggcaacg tgggtaatgg ccttggcgtg acgctggcaa
    14101 cgggcgaccc gagcattatt gcaccgacta aaactatcgt cagaactggc gagaagatga
    14161 ttatcgacgg cctggagttt gacttcctga tgaccccagg tagcgaagca ccagccgaaa
    14221 tgcacttcta tattccggcc ctgaaagccc tgtgtaccgc cgaaaacgcc acgcataccc
    14281 tgcacaactt ctacactctg cgcggcgcga aaacccgtga caccagcaag tggaccgagt
    14341 atctgaacga aacgctggat atgtggggta acgacgcgga agtgctgttt atgccgcaca
    14401 cctggccggt ctggggcaat aagcatatca atgattatat tggtaaatat cgcgatacta
    14461 tcaagtacat tcacgaccag accctgcacc tggcgaacca gggctacacc atgaatgaaa
    14521 tcggcgacat gattaagctg ccgcctgcac ttgccaataa ctgggccagc cgtggctatt
    14581 acggttctgt cagccacaac gcccgcgcgg tgtataactt ctatcttggc tattacgacg
    14641 gtaacccggc taacctgcat ccgtatggtc aggtagagat gggcaaacgt tacgtgaaag
    14701 cgctgggcgg ttctgcccgt gtcatcaacc tggcgcaaga agcgaacaag caaggtgatt
    14761 accgctggtc ggcagaactg ctgaaacagg tgattgccgc caacccgggt gaccaggtcg
    14821 cgaagaatct gcaagcgaat aactttgaac agctgggcta tcaggccgag tccgccacat
    14881 ggcgcggttt ctacctgacc ggcgcgaaag agctgcgcga aggggtgcat aagttcagcc
    14941 acggcaccac cggttccccg gacaccattc gcggaatgtc ggtcgaaatg ctgttcgact
    15001 ttatgtccat tcgcctcgat agcgaaaaag ccgcgggtaa aaatatcagc ctgaacttca
    15061 atatgagcaa cggcgataac ctcaacctga cgctgaacga tagcgtgctt aactaccgta
    15121 aaacactgca accgcaagcc gacgcctctt tctacatcag ccgtgaagat ctgcacgccg
    15181 tgctgaccgg acaggcaaaa atggcggatc tggtgaaagc gaagaaagcc aaaattattg
    15241 gcaatggcgc gaaactggaa gaaattatcg cctgtctgga taatttcgat ttgtgggtta
    15301 atatcgtaac cccaaattaa tgtcactcgc ttttgggggc
//
LOCUS       DAZUCE010000115        15068 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZUCE010000115 DAZUCE010000000
VERSION     DAZUCE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.115"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(71..481)
                     /locus_tag="ACHY03_003024"
     CDS             complement(71..481)
                     /locus_tag="ACHY03_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFS3906226.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAQLARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(780..2162)
                     /gene="yahJ"
                     /locus_tag="ACHY03_003025"
     CDS             complement(780..2162)
                     /gene="yahJ"
                     /locus_tag="ACHY03_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFS3906227.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGDKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMVAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDATFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(2172..3122)
                     /gene="yahI"
                     /locus_tag="ACHY03_003026"
     CDS             complement(2172..3122)
                     /gene="yahI"
                     /locus_tag="ACHY03_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFS3906228.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQKGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(3172..3345)
                     /gene="yahH"
                     /locus_tag="ACHY03_003027"
                     /pseudo
     CDS             complement(3172..3345)
                     /gene="yahH"
                     /locus_tag="ACHY03_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahH"
     gene            complement(3359..4777)
                     /gene="yahG"
                     /locus_tag="ACHY03_003028"
     CDS             complement(3359..4777)
                     /gene="yahG"
                     /locus_tag="ACHY03_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFS3906229.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVAQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(4777..6324)
                     /gene="fdrA"
                     /locus_tag="ACHY03_003029"
     CDS             complement(4777..6324)
                     /gene="fdrA"
                     /locus_tag="ACHY03_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFS3906230.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLAEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGTKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(6314..7177)
                     /gene="yahE"
                     /locus_tag="ACHY03_003030"
     CDS             complement(6314..7177)
                     /gene="yahE"
                     /locus_tag="ACHY03_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFS3906231.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFRLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVIDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALILFISGHPAGKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(7217..7822)
                     /gene="yahD"
                     /locus_tag="ACHY03_003031"
     CDS             complement(7217..7822)
                     /gene="yahD"
                     /locus_tag="ACHY03_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFS3906232.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKNCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            8080..8577
                     /gene="yahC"
                     /locus_tag="ACHY03_003032"
     CDS             8080..8577
                     /gene="yahC"
                     /locus_tag="ACHY03_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFS3906233.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTTGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLATVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            8669..9601
                     /gene="yahB"
                     /locus_tag="ACHY03_003033"
     CDS             8669..9601
                     /gene="yahB"
                     /locus_tag="ACHY03_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3906234.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(9643..10731)
                     /gene="pdeL"
                     /locus_tag="ACHY03_003034"
     CDS             complement(9643..10731)
                     /gene="pdeL"
                     /locus_tag="ACHY03_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS3906235.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(10873..14856)
                     /gene="ehaA"
                     /locus_tag="ACHY03_003035"
     CDS             complement(10873..14856)
                     /gene="ehaA"
                     /locus_tag="ACHY03_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFS3906236.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LALSKDVAVSEDHTAVFGKDNQTIDYRISVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SEKKITLNHTDKDATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVELTGNNYAFSGYID
                     VASGAVAVISEDKNIGRAELDVDGKLQINANKDWVFDNDLEGKGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQKGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGHQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVHAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TDYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         3475 a         4068 c         3574 g         3951 t
ORIGIN      
        1 aaacagcaaa ataaacaaaa ttagcgatat tttatgtttg tgagcttatt tcgtgtagat
       61 aaatagttat ctacacaacg tcaataaggg catattcttc aaagataacg tccatttctg
      121 gtgaatagct tccttctgcc tggaaaaaac gacggtgttc attacgccaa tattcaagac
      181 tgaggtcacc ttcaccttcc ttacgggcaa gctgcgcggt gacatcagaa aagcgcagta
      241 aatgcaggcc aatcactctg ataacacata caggctcacc acggctattt tctacaatct
      301 tatactcacc aatcatcgga aatgcgttgt cttcaataca tcccgccagc gaaccacagg
      361 aagctgtttt aacaccagtc gcaatcaacg ttgcgaggtg atcggccaac gcggctgaat
      421 cgccaaaagt acaaaaggaa gcatggggat atttttgtga gaatttttct tttatggtca
      481 tggcaatatt tttgcgatta cggaaaaaca aatcacatct taattactgt tggtaaccat
      541 cagcaagagt atcattggtt tttcgatact gaatcttttt tatccgaagc aggtcacatt
      601 tatggcctga taaatacctt tgttaaaact cacttttgtg tatttctctc aaataatccc
      661 ctggaaatta tgccgggaga ttataaagtt caggatcatt cattagcaaa ccattcccgc
      721 cccactccct ggggcttaaa tctcttacta ttcaccgttg caggcgtttc aaatgcgcat
      781 caacctgcca cactccccca caccagttgc cctttatgga acgttgcggt tctcggcgag
      841 atacgcgcca ccgcctcggc ggaacaggag gcgtccacca gcacaaaggt ggcgtcatcc
      901 tgcgctttgg gccagacgcg ctcgcctttt tcgttgagcg gcaatacatc accagtggct
      961 aaaaacagcg agcgggagag gttctgttcg ttaggacgaa tatagagctg cgcgtagaga
     1021 ttggcttttt ccagcatgtc gcccagacca taaggcgacc agtggtcgat aacgctgtca
     1081 gtgccagtca tcacttttac gcctttgtcg tgcaactgtt tgagcggcat atgcagcgtg
     1141 ccaatcggca ccgtcgaggc gatagaaatt tgctgcgcca ccatccggtg cgccagttca
     1201 tctacctgtt gttcgttgag cgttgccagc gcaaaggcgt gactgatggt cagcttgccc
     1261 ttcagctgtg gcgttttctc taccgtttca accatataat tgatggctgc cacgcctgcc
     1321 ggagtggttt cgtgcaggtg aatatcgacg cctttgtcgt agtccagcgc aatctggaac
     1381 atggtgtcga gggatttttc catcgcgcca tcgacactgg tcgggtccag cccgccgacg
     1441 taatgcgcac ccgcctgcat tgcttcacgc attaagggtt ccgatttcga cagcagcaaa
     1501 ccgtgctgcg ggaaggcgac gatttcacac tcgaagcccg cctgacgtcg cgccagcacc
     1561 gcctgcaaat tttgcagatt tttcaggccg gaaactggtt cgatattgca gtggctgcgg
     1621 gcaatggtag tacctttcga ctgcaataga tcgatcagtt tttccgcccg ctcctgcgta
     1681 tacggttgta actccggcag cattttctgc tcgagtttga tcatgtcctg gatggtggtg
     1741 cctgccgggc gattgagcga gcgccacggc ccgccgtaaa aggttttgtc gagatgaata
     1801 tgcatgtcgc gggtggtggg cagcatcagc ttatcgccag cgtcatagtg cggcagcgtg
     1861 gcatccgggt gctgcttatt ctcgcgcagc gcgacaattt tgccgttctg aatctccacg
     1921 gtctggcgcg ccgtgcgggt ctgcaccgcc acgccatttt cgtagtcaaa tccagtttcc
     1981 agcagcacgt tgtcgagata gtaatgcggg tcagtgattt tcatggtgtt gttcgcttcg
     2041 cagtttgtgg tgccagagac cgccgcatgg gcgagcggca cagaggtacc aaacagcgcg
     2101 gcggcggtga ccatcttacc gctctggctc aaaaactcac ggcggctatt gttttctttc
     2161 atcttacgtc cttacgtttt aataatgtgg gtgcccgttt caccgcgcag cgccgcgggc
     2221 aggcattccg gcgtggtgat aatcacttct ttaccgccct gttccaggaa cgtcaggctg
     2281 gcgatgattt ttggcaacat gctgccgggc gggaaatgtc cttcctgcat atagcgagtc
     2341 atggtggcaa tatccacccg atccagcgcc tgctgctcgg gtttaccaaa gtggatacag
     2401 actttttcga cgccagtggt gatcacaaga atgtcggcgt gaatttcacg ggccagcagc
     2461 gcggtagaga gatctttgtc gataaccgcg tccacgcttt ggtaatctcc cgcgtcagta
     2521 cgcactaccg gaattccacc gccgcccgcg ccaattacga caaaaccttt ttggatcagt
     2581 gctttaatgg caggtgcttc gacaatacgt ttcggttccg gcgaggcgac cacgcggcga
     2641 tagccccgcc cggcatcttc aacaaaacac cagtcagggt ttgccttttg taattcgtca
     2701 cgctggctgt cactaaagaa tgcgccgatg ggcttggtgg gatgggcaaa acctggatcg
     2761 tttttatcca cttccacctg agtcaccacg gtgacggctt tcttctcgcc gtgacgcgcc
     2821 agccggttat tcagcgcctg ttggatcaga tagccgatgc cgccttgcgt atctgccaca
     2881 cagttcgcca gcggcgttaa gggcagccct tcgcgctcgt gggcaatctc cgcacggcgt
     2941 aaatccagcc cgacctgcgg cccgttaccg tgggtcagca caatgtcgta atcggaagcc
     3001 agcatttcca gcaccgtatc ggcgacggct ttcaccgcct ccgcctgatg ctcaatcgac
     3061 tggctggcgt tatctttgat aatgctgttg ccaccaatgg caacgaccac aagttctttc
     3121 atgttgttat gtccgctaca gagggtgatg gggttatgac caacgccaga ctgcctgatg
     3181 cgctacgctt atcaggccta caaattctcg cgccactcgt aggccggata aggcgttcac
     3241 gccgcatccg gcaatggtga caaatgcctg atgcgctacg cttatcaggc ctacagattc
     3301 tcgcgccatt cgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa catgtaggtc
     3361 agacgccgta acgttcgcac cagccaagaa tggctttctc gaagcaggca aaaggtggat
     3421 gcacaatgcc cgcaccaatc atgccgacgc ccgcatcttt atgggcgatg gcggtgttga
     3481 tcactggcag aatgccgctg ctacccacgc gggtgatgtc gattgccgac ggcacgccca
     3541 taaaaccgag cagcggaatg gtgacgttgg ggttttcgcc aagggtgatt tcgcgcatct
     3601 gacgggagaa atcgatagcc tcttccaccg tgccacccac cagcgcgacg attgcaggcg
     3661 cggtcgccat ggcaaacccg ccaataccgt aggtttctgt gatggcgctg tcgccgatat
     3721 ccagccccga atcttccggc ttataacctg caaacatcgg gccgatgacc tgctgcgctg
     3781 ggccggtaaa ccactgcccc ggcaggccgc tgacccgcag gccgaactcg acgccgttac
     3841 gcgccatggt ggtcaccacg gtgctgtatt cgatgccgtg cgccgcatcc atcgccgctt
     3901 tacacatcgc catccacgtc gggccggaga agtaatcgct actggcaaca aactcaaaca
     3961 cttcgcgctg ttgcgcgacg gaatagcccg cctggataat ccccggcgtc agcgcctgaa
     4021 tcagcagcgt cgtcccggcg ttattgcggt tatggcactc atcgcccata tgcagcgcct
     4081 gcgccagcat taagcgcaga tcgatttcgc cgataatttt catcgcgtcg cgcagtattg
     4141 gtccctgcac atcacgcatc cagttcaggc ggtcaatcac gctctggtcg ttagcgccca
     4201 tacgcaaaat cttcgccatc tgctcgctca tgttggtata agcgatgttg ccgtaggttt
     4261 tgttttttac gatgtgcata aacatcgagg ccgaggtaac acccgccatc gatcccacgc
     4321 agtcgtgctc gtgacacggc gagaaggtga tctccccgga agccgccagt tcagccgcct
     4381 cgtcgagatc tttcgccagt ccttcgaaca ccagcgctcc ggtgaccgcg cctttcatcg
     4441 cgccgcacat tttttcccag gtgatcggcg gcccggcgtg aagaatggtt ttcggcgtca
     4501 tgcccggcac cacgttaatc gcctgatcaa aaccaatcag caccggatgc gactggataa
     4561 tgcgctccag cgcctgctgg ttagcggcgg cgattttgtc tgccagcggc gaatcggcaa
     4621 tgttatccag cgcctgcacc acctgcatat tgccctgccc cggcggcgtc cagtcgagtt
     4681 gagtcacttc tacatgctgc tttttcaggt catcgctaaa catggcgatg ccgacgttaa
     4741 taacgttcaa tggttggcta aacagtgact ggctcatcag gcttcctccc ctttgcagat
     4801 aaattcacgc gccagcaatc cggtattggt gctgctgctc gccagaatca ctccggcatc
     4861 aagcagcatc tggctttgtt gttctaacga tggcgtatca agatcggtac ccagcacata
     4921 ggcgagaatg atcaactcgc gtccttcggc agcggcgatc gctttcgctt ctttgatcgc
     4981 ctcgatggtg gagccgaccg gatcttcatg cgatccaaat ccgagcacaa aatccatcac
     5041 gatcaccgcc acttctggat cgcgcgcctc ttcgatcaag cgactgatgc ggttggtggg
     5101 gtcaatcatt ggatgcggct tgccgttggt gaagtcgtca tcgccaaagt cgaggaaggt
     5161 gtgtttgatg ctgcggttga tatctttcag gcggaattca ggatcgggct ggatgttgct
     5221 gtagacatcg ccgtgttttt ccatcaccgc gaacatggtt tcgtcacaca gcgtaccgcc
     5281 gcagaacagg ccacgaatgt atttctgctg gggttgcaga cgcgcacgca catccgcaat
     5341 caacggctgg ttcagggtat gcaggtcgag attttcctgt ttcacgccgg agagcatcac
     5401 cgctttcagc gccgcttctt tggtgccgcg ggcaaactgt agcccctgct cgtcaaccgg
     5461 cgtttcgcca cgaccgagga agcagaccac caccggcttg cggcaggcgc gggcgcgttc
     5521 cagcacttta cgggccaccg caggcgcggg aggttttgaa atcagcgcga taatttcagt
     5581 ttgcggatcg ttttccagca tcccgatggc gtcgagcatc atcaggccgc cgattttctc
     5641 gctcaggtcg cgcccgccgg tgccgatgag ttgtgaaacg ccgccaccaa attcatgaat
     5701 gcggacgctc agctcctggc tgccggtgcc ggatgcgcca acaataccga tgttgccgcg
     5761 acgcacggcg ttaccaaagc agagcgccgc accgttgata atcgccgtgc cgcagtctgg
     5821 ccccatcatc agcagccctt tttcgtgggc cagttgcttg agcgccagtt catcttcaac
     5881 tgagacgtta tcggaaaaca gcatcacgtt gagatcgttt tgcagcgcct gacgcgcttc
     5941 gcgagcggca aacagaccgt tgaccgaaat caccgccagg ttactttccg ggatatgctt
     6001 tttggcgctg gcaatagtgg cgtaacgcgc ctcgtgcgag ccgctttgcg ctttggtgtt
     6061 gaacagttct tcaatctccg ccagcaactg ctcattgtcc gcacccgatt taccattgat
     6121 gacaatcatc aggtcgccgt ttttcgcctg ctccagctcc ggcgtcagca gtcctaaatt
     6181 cttcagcacg cctttattca tttcggtcgc catcgccaca aatgcctgct cgacgccgtc
     6241 gagtttattt gcacgtgtgg agatagacat cagcgagaca gaatcaaaat aggtattcgg
     6301 tttaatgact attttaactg acataattcc ctccgtaggt ttggctcagc gcacaaccat
     6361 cggccatgcc atacagcatg tcgcagccgg aactggagcc aatatgttta attttttcta
     6421 ttgcctgagt tgcgttccca gggatgtcat aaataatgtt gtgaataaaa cgatgaatat
     6481 tttcccggca gcgttgttgt aatgcggctt ccagcgttat ggcacttaat aacgtggtat
     6541 tatttctgcc gcgttgcaag ccgagataaa attcctcttt gtattttccc gccggatgcc
     6601 cggaaataaa taaaataagt gccagacctg ttaaataatc atcggctgat ggcgtaaggc
     6661 caatccctaa gcccatcata ctggcgaccg ctgaggcgat attttgttta tcgattactg
     6721 cctgtataac tgcccgtcgt ttaatatgta attctttatt taacgcctga taaaacggat
     6781 tatcgccgcg ataattaaac agggtttcat tttcccccag gtgctgatga atgatgtcgt
     6841 gccactgttg ccaggcgata aggcgaaaat tcacagcggt catttgcaag gttggggaca
     6901 gccaacgccg acaacgactc atctctatat gaagatgttg accaaccgta ataccttgat
     6961 cgtcaaactg aaccttatca ccatgccgga acagatcatc aaagtgagtg agtgccaacc
     7021 gacaactgtt tggcgcattg tcgtactctt cacaaagcag cgtcagcaac tgctggcgag
     7081 ccgggagtgc gatatttacc gctctggcaa agacctgttc aacctgtcct tgcccccgct
     7141 gcgccagaaa atccgcatcc gcagttagcg cccacacaca ccgtttttct ggtgtgttga
     7201 tagcaagcct cccggtttat gcacctgcgg caatcagtaa ctgcgcaatc tcttcaaagc
     7261 cccgttcccg cgccagttcc agcggcgttt tgccatattt atcggtcaga tgcgggctgg
     7321 caccgtgttc cagcaataac tgcacaatcg cctgctgttt aataccacca tcattaagca
     7381 caatcgcttc cagtagcggc gtccagccta catggttggt ctggttaacg ttaatctccg
     7441 tatgcgccaa aagctctttt acaatactta aatggccttt ttcacaggca ggcgtcaggc
     7501 cgacaccgcc aaaacgggtt acgcaattaa gatcgggttt agccggtaaa ataattcgta
     7561 gtagcgttaa atcatcgttc agacagctaa ttaaaaaagg atttaaacag gtatgatctt
     7621 gtttattaat atccgctccg gcgtcaatta atgcctgaac gcaagcatat tgctgatata
     7681 aacttgccag cgtaattgcc gttttcccct gacggtcgca ggtattaata tcgacaccca
     7741 gcgcaaggca gttttttact ttatcgatat caccctgttg tgcagccaat aaatagtcgg
     7801 caggtagatt ttttatactc attcacattc ccctggtttt tgtggctgtt ttcgatgaat
     7861 tgagaatagc aacgataatc tgataaaaga acccgcttaa atttaattcg ccgtatttcg
     7921 cctttttgtt gaatgatgtt caacaatctt ttcgcagtgt gcggtagtgc aatatttgtt
     7981 tctttgaccc gatacccttt cactcaatca ggttttattt ctctatagtc tgcacatgta
     8041 agagtagctt tcgtccatgt ctaaccagag gggaacaaga tgaacggact cacggcaacg
     8101 ggtgtcaccg tcggtatttg tgcagggctt tggcagttag tgtcttcaca tgtcgggctc
     8161 tctcagggat gggaattatt aggaacgaca ggcttcgtcg ccttttgcag tttttatgcc
     8221 gcaggcggcg gtaaatcggg gtttatcaga agtctggcgg ttaattattc cgggatggtg
     8281 tgggcgtttt tcgccgcgct gactgccggt tggctggcca ctgtaagtgg actgtccgcg
     8341 ttctgggcga gcgtaattac taccgtaccc ttctcggcgg tggtggtctg gcaagggcgt
     8401 ttctggttac tgtcatttat tcccggtggc tttctcggca tgacgctgtt ttttgccagc
     8461 gggatgaact ggacggtgac gttactgggt tttctggcgg gtaactgcgt ggggataatt
     8521 tcagaatatg gtggacaaaa attaagcgaa gcgacgacta aacgcgacgg gtattaaaag
     8581 ttattcaaaa atgattatcc atctttaacc gatacctcta cagtcatagc tggagaaatt
     8641 catagtagat aatggcgaga atggtttaat gaactcaatt tttaccgaag agaatctgtt
     8701 ggcatttact accgctgcgc gttttggcag tttcagtaaa gccgcagaag agttgggttt
     8761 aaccacttcc gccattagct acaccattaa gcgtatggag acggggctgg atgtggtgct
     8821 gttcactcgc agtacccgca gcattgagtt aacggagtcc gggcgttatt ttttccgcaa
     8881 agctaccgac ctgctgaatg atttttatgc catcaaacgt agcatagata ctatttcgca
     8941 gggcatagaa gcgcgtgtgc gcatttgtat taatcagctg ctttatacgc ccaaacacac
     9001 cgcgcgctta ttacaggtgc tgaaaaagca gtttcctacc tgccagatta ccgtgacgac
     9061 cgaagtgtat aacggcgtct gggatgcgat tattaataat caggccaata tcgccatcgg
     9121 cgcacccgat acactactgg acggcggcgg tattgattac actgaaattg gcgcgattcg
     9181 ctgggctttt gctatcgccc cggatcaccc gttagccttt gtgccggagc cgatcgccga
     9241 aagccaactg cgtctctacc ctaatattat ggtggaggac accgcgcata cgattaataa
     9301 aaaagtgggc tggttgctgc acgggcagga gtcaattctg gtgccagatt tcaacaccaa
     9361 atgtcagtgc cagattttag gtgaagggat tggttttttg ccggattaca tggtccgtga
     9421 ggcgatgacg caatccctgc tggttacccg acaaatccat aatccgcgcc aggattcgcg
     9481 catgttactg gcgactcagc attctgctac gggtcaggtg acgcagtgga ttaaaaaaca
     9541 atttgcgcca aatggcatat taacggggat ctatcaggat ttactgcatc gggagaatta
     9601 atcgctgcca acctcccgat gcgctgtgaa tagcggttta catcaaccac ctgctttcat
     9661 tacccattca gagataaatt tattacccgg tactggcgga gagtacaaat agccctgcaa
     9721 aaagtgaacg cctttaccga tcattaaatc cgcctgctcc tgggtttcta ccccttccgc
     9781 cacgatactc agaccaggct tacgcgccag ttcgacaata ttgtccacaa tatgaccgga
     9841 tatttcgtcg acactcgcca tttgcacaaa tgacttatcg atcttaataa aatcgaccgg
     9901 aaacgcctgc agataacgat aggtcgcata acccgtacca aagtcatcaa gcgcaaaggt
     9961 aatgttgtgc tgatgaaggc tgtcaaatat cgctctggct tctggcgtta ccggaatagg
    10021 gttacgttcc gttagctcga gaaccagttt gattttatcg tttcctaatt tcttgaccag
    10081 gttcaggcat tctttttcaa atcccgcggc caaaaaacaa cccgccgaga cgttgatacc
    10141 aatatggaaa ttatccggca gcaaatgttt taccggcatc agaatatccg cagtctgttt
    10201 catcagttgg cgggtcatta tgacaataag accggatgac tccgccagag gaataaactg
    10261 atccggtggg ataattcccg tttgtggatg ttcccagcgg acaagcactt cacagcccgt
    10321 cagtacccca gtctgcgcgc agaaaaccgg ttggatccac ggtttaaatt catggttttc
    10381 caacgccaga ctgatggctt caggcgtgac gatatggtga caacggttat cagggatata
    10441 aaaattattt ttatttcccg tgccactaat gacttgcgga tgatattgaa aaaagatatc
    10501 ccgccagaag gtaatatcac tctggatgcc tagtttttca tacagctgct tcttttgatg
    10561 tgaaattgtc tttgcactgc gatttctgta ctgtgagatt tctgtaaccg acattccctc
    10621 cattagcagc ttgagtaaaa gccgttcttt ttcggacacg ataccaggca acgtcaaatt
    10681 caccgttgcc ccgaactctt ccaaaaaaac actcaaatca tgcgaagcca ttgaacacac
    10741 cttgattaaa tcttgtgcgc cagctaaatc tgcgagtctt cactctgacg gataaagctg
    10801 gctgcaagaa aatgacacag agagccgaat tatctcccgt tcggctctct ctccttttac
    10861 tcattattca gatcagaatt gccacttaat gccaaccatt gcagaggtgt cattatatcc
    10921 cctatcggca acctgaacgc ccacattccc ccagacattc aggttggcat ttagctgtcc
    10981 ttccaccccg gtttttatct cagcaatatt tcgggctcca tcctgagtga cggacacgcc
    11041 atccatactg gtgctgaaat ccttactgtt atgcagccag ttcacttcta caaacggctg
    11101 gaactcgcgg gatttaccgt catccatttt atggtggctc ttcagccagg tttttacgcc
    11161 aagtcgggtt tgaacattgc catcaccgtt gctatgaacg agggttccgt tgctttcgcg
    11221 gtgcttatcg gctttgactc ccatccaggt aacctgtgct tgcggctgaa cataccattc
    11281 attacgcgtt ccctggctgc cattaaattc agctaatttg tgtttgtatc cagcttccag
    11341 tgaagcggta aatccttttg atttatagga ttcactttgt aagtcatccc ctttcactgt
    11401 gttatcaaac cagctgtact gcgcccaact gtcgagatac gcgccattac gcgactcgtc
    11461 atcggcatac caggtggcat agaggcctgt gctatatccg ttcacactcg cttttgcacg
    11521 ataaccggtt cgcgaggaaa tggttttgct gtcgctgttg ccatatcccg ccatgacccc
    11581 aacatgccag ctgtcgctgc cgttttggct ccactgcgcg acatcgcctc ccagttgcag
    11641 aacatagcga ttgctttggg ttttcagctg gccgctgcca tcacgccatt tattatgacc
    11701 accttcatgg cgcatccaca tagtggtttg tttctgctca cccgtcacca tgtcggtata
    11761 gtccgtattt cccaaacgct cgtgcagacg cgtggtgaac atggtattcg ctgctgcaag
    11821 gttcgcaatg tagctacccg cctccggtcg caggtcatta tccacattca gatccggacc
    11881 cggcgtaggt gtcgggttag ggttcggctc tggattcggg tttggctccg gattcggcat
    11941 cgggtctggc tccggctgca gttcaggaga atcactgccg ctggtcagat accagttacc
    12001 actatttgcc ccttgtccac gcgcgagagt gtagtcatac gcccccgcaa cgatacgacc
    12061 agcatgaaca aattcgccct cagacttacc gtctacatgg ataagttcga taccgttaag
    12121 ggttttcgca cctgtaccgc cagcgttatt caccgtaacg gcagtcgtgc cgctagtatc
    12181 gccctcgaca accaatttat cggttactga gtcatcgcca ttcagtaccg tattcattac
    12241 tagttgaccg ttattaccgt ggtagttccc cttcaccact aacacattgc cagcttggtg
    12301 tcctgacatt ccggtcatca gcgtaccgtt gttggtcaga ttgccgccca gggtgtaatt
    12361 cacagacgca gccgctcttt tttcagcgct attattcaac gccagggtac cgttgttctc
    12421 aacgtttgcg ctacccaatc cctgcgcatg acttaccagg acattagccg aattagcaat
    12481 gttcacattc gccgtcaggt tgctgttatc gcccgcgatg tccagcgtgc cgccctgaat
    12541 attcagctct ccgctccccg ttaacgaacc ggttgaagtg cccccgttgg taaccgtcag
    12601 actgccgcca tttaagctca gcagtgaatc ggcgctgcca ttgagcgtgc ccacggtctg
    12661 gctgtgacca ttcatatcca gttcagtctc tgccgccaga cgcagttcat gggtattacc
    12721 caacacgtta tcgttattca acaaaagcgt cccactgcgc agatcggtaa caccggtata
    12781 gtcgttgtct ttgttagaaa gcgatacggt ctgacccttc tggctgctga atgccaggtc
    12841 accactgccg gtaatctttg cgctgagatc ggcggcattc tcgcttttac cgttagagct
    12901 cagcaccaga gcatcgctgt cggtagcgtg taaatcaagc tgggtcaggc cgtaaccgat
    12961 atacagaccg tcattgttta ccccactcgt cagacgatag tcgtaagtgc cgacggccgc
    13021 tttttgccca ttctgctgaa tatcacgttg ggcactgctg gagaggattt gcccattttt
    13081 atcctgcaat tgcagattgc ccgcatcgcc cagaacttcc gcaccttgcg catcaaccaa
    13141 cttaatggtg ctgttaccat catcgacttc agtgagcgat aacgcagagt caatatcacg
    13201 agatactgag cttacaacgt cactgtcaga aacctgaatc acaccctcgc cgcgcaaatc
    13261 cagcgtttta ctaacgttga cggtcccttc tgtcatctga gcacctgctg tcagggcacc
    13321 aaaatcaacg gttccgccgg agaatcccag agtgctaata gaatgagcgc ccttacccac
    13381 tgttacctgg cttccctgac ccgcagtgat cccacctttc tgcagaaact cagcattctt
    13441 ttccaggtta aatgtcgtgt tctggaacgc cagtgaaccc tggaaccagt ctgtataagc
    13501 aaactcatca aaggagaatt cgtgattccc catgtttatt tcaacaatgc ctttaccttc
    13561 aagatcgtta tcaaataccc aatctttgtt ggcattaatt tgcaatttgc catcaacatc
    13621 cagctctgca cgaccgatat tcttgtcttc agaaataaca gcgacagcac cagaagcaac
    13681 atcaatatat ccgctgaagg catagttatt gccggtcaac tccaccgtcc cactgtcgat
    13741 acttattgta ccgttgccgg aaatcttatt ggcaaaggtc atgctatcgt cagccttcag
    13801 gtgtaatttc ccgcggttgt tgatctctgc tgtacctata ttttgggtga tccctaaagc
    13861 gccattttgc tcaattttga ctttaccact aaaggcagag ttatcaccgg ttaagtaagt
    13921 cgtgccattg agcgcattga tatagcccag gccctcgctg ccactgacaa tatccgcgga
    13981 aatagtcgcg tctttatccg tgtggtttaa ggtgattttc ttctcggatg tctttgccca
    14041 cacaaactca attttttcgg catcaataat ccctgccgcc ttcgcagcac ttccttcctg
    14101 tgcacctaac gctaactcag agttggtgct taaactaatt ttcggtgcgg atattttacc
    14161 tccatctgat acgactaatg ttgtatcaga acgttggtcg taataaccca ggctaatatg
    14221 ttcacgagca ttaacttcac caccgttatt aaccgacagg aactgattac cgccatatcc
    14281 gccgtaaacg cccataaaga aggtcccggc atttacctga ctaccttcgc cgcttacatt
    14341 cccctgcgta taagtcagac gactggtgcc cttaacatga attttgccgc cattggagac
    14401 attaaaatag ccttgatcat gtctcatgcc tgagtttgac tcatacaaat catcagcata
    14461 taactcgctg ccagcattgg agacattcag gaagcccgta cctttcagca aaaagttaag
    14521 tgggccattg attgttacct ttcctccggt aatatccaac ccacccccag acgcgagagt
    14581 cagacgcgga cgggaagtat ctgtcgcatt gattaccaga ttggcattgt cggtcactga
    14641 aatacggtaa tcaatagtct ggttatcttt accgaataca gctgtgtgat cttcacttac
    14701 agcgacatct ttagacagtg ccagagatga atataaactt atcgcgctaa ttaaaacagc
    14761 ccgggacttt tttcctttta ctctgctccc cagctcagag cagacgctcc aacagttacg
    14821 ggacctgttc cactttagcc gatatatttt attcatcaca atctccagca atacatccaa
    14881 ataaaaccaa gattgcataa acagcaacgc attaaatgcc attccggtta ctgttcatcg
    14941 catataaaaa ttcaaaaatt ttgcatagca ttggcaacca catatctgag tatgtagttc
    15001 agagggtaat aaaatagcaa gttactactg aaaacttatt ttaagttaac actcttaact
    15061 ttatcatt
//
LOCUS       DAZUCE010000116        14988 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZUCE010000116 DAZUCE010000000
VERSION     DAZUCE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.116"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            138..1196
                     /gene="citC"
                     /locus_tag="ACHY03_003036"
     CDS             138..1196
                     /gene="citC"
                     /locus_tag="ACHY03_003036"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFS3906237.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTKG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1211..1507
                     /gene="citD"
                     /locus_tag="ACHY03_003037"
     CDS             1211..1507
                     /gene="citD"
                     /locus_tag="ACHY03_003037"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFS3906238.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1504..2412
                     /gene="citE"
                     /locus_tag="ACHY03_003038"
     CDS             1504..2412
                     /gene="citE"
                     /locus_tag="ACHY03_003038"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFS3906239.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2423..3955
                     /gene="citF"
                     /locus_tag="ACHY03_003039"
     CDS             2423..3955
                     /gene="citF"
                     /locus_tag="ACHY03_003039"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFS3906240.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQNGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQESGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3959..4510
                     /gene="citX"
                     /locus_tag="ACHY03_003040"
     CDS             3959..4510
                     /gene="citX"
                     /locus_tag="ACHY03_003040"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFS3906241.1"
                     /translation="MLLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPFPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALCALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCAQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4485..5363
                     /gene="citG"
                     /locus_tag="ACHY03_003041"
     CDS             4485..5363
                     /gene="citG"
                     /locus_tag="ACHY03_003041"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFS3906242.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRSN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5414..6877
                     /gene="citT"
                     /locus_tag="ACHY03_003042"
     CDS             5414..6877
                     /gene="citT"
                     /locus_tag="ACHY03_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFS3906243.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            6991..7797
                     /gene="rna"
                     /locus_tag="ACHY03_003043"
     CDS             6991..7797
                     /gene="rna"
                     /locus_tag="ACHY03_003043"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFS3906244.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVNRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8026..8436
                     /gene="rnk"
                     /locus_tag="ACHY03_003044"
     CDS             8026..8436
                     /gene="rnk"
                     /locus_tag="ACHY03_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFS3906245.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8574..8663
                     /gene="yldA"
                     /locus_tag="ACHY03_003045"
     CDS             8574..8663
                     /gene="yldA"
                     /locus_tag="ACHY03_003045"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFS3906246.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8667..9905)
                     /gene="ybdR"
                     /locus_tag="ACHY03_003046"
     CDS             complement(8667..9905)
                     /gene="ybdR"
                     /locus_tag="ACHY03_003046"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFS3906247.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYIRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10126..10554
                     /gene="uspG"
                     /locus_tag="ACHY03_003047"
     CDS             10126..10554
                     /gene="uspG"
                     /locus_tag="ACHY03_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFS3906248.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10675..12240)
                     /gene="ahpF"
                     /locus_tag="ACHY03_003048"
     CDS             complement(10675..12240)
                     /gene="ahpF"
                     /locus_tag="ACHY03_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFS3906249.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12369..12932)
                     /gene="ahpC"
                     /locus_tag="ACHY03_003049"
     CDS             complement(12369..12932)
                     /gene="ahpC"
                     /locus_tag="ACHY03_003049"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFS3906250.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13304..14050
                     /gene="dsbG"
                     /locus_tag="ACHY03_003050"
     CDS             13304..14050
                     /gene="dsbG"
                     /locus_tag="ACHY03_003050"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFS3906251.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14259..>14988
                     /locus_tag="ACHY03_003051"
     CDS             14259..>14988
                     /locus_tag="ACHY03_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3906252.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAAVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDL"
BASE COUNT         3632 a         3912 c         3714 g         3730 t
ORIGIN      
        1 tatagcgaaa ggagaaaaag atatacctcg atcacccccc tttctcccaa gtgaaaataa
       61 aaggttatca gtttgcaaca ttgaacaaca ttcgttgcaa atcgataaca acatgtacct
      121 tcaggatact atttattatg ttcggcaatg atattttcac ccgcgtaaaa cgttcagaaa
      181 ataaaaaaat ggcggaaatc gcccaattcc tgcatgaaaa tgatttgagc gttgacacca
      241 cagtcgaagt atttattacc gtaacccgcg atgaaaagct tatcgcgtgc ggtggaattg
      301 ccggaaatat tattaaatgc gttgctatca gtgaatccgt tcgcggtgaa ggactggcgc
      361 tgacattagc cactgaactg ataaacctcg cctatgagcg gcacagcacg catctgttta
      421 tttataccaa aaccgaatac gaggcgctgt tccgccagtg cggtttttcc acgttgacca
      481 gcgtaccagg cgtgatggtg ctgatggaaa acagcgccac gcgattgaaa cgctatgccg
      541 aatcgctgaa aaaatttcgt catcccggga acaagattgg ctgcattgtg atgaacgcca
      601 atccctttac gaagggtcac cgttatctga ttcaacaggc tgcggcacag tgcgactggt
      661 tgcatctgtt tttagtcaaa gaagattctt cacgcttccc ctatgaagac cggctggatc
      721 tggtgttaaa aggcaccgcc gatattccac gcctgactgt gcatcgcggc tccgaataca
      781 tcatctcccg cgctacgttc ccttgctact tcattaaaga acagagcgtc attaaccatt
      841 gttacaccga aattgatttg aagattttcc gtcagtacct cgctcccgcg ctgggtgtaa
      901 ctcaccgctt tgtcggtact gaaccctttt gtcgcgttac cgcccagtac aaccaggata
      961 tgcgctactg gctggaaacg ccgactatct ccgcaccgcc catcgaactg gttgaaattg
     1021 agcggctgcg ttaccaggag atgccgatat ccgcttcccg ggtacgtcaa ctgctggcga
     1081 aaaacgatct cacggctatc gcgccgctgg tccctgcagt cacgctgcat tatttgcaga
     1141 acctgcttga gcactcccgc caggacgcgg cagctcgtca aaagaccccc gcatgagaaa
     1201 caggtgaaaa atgaaaataa accagcccgc cgttgcaggc acccttgagt ctggggatgt
     1261 gatgatacgc atcgccccac tcgatacgca ggatatcgac ctgcaaatca atagcagcgt
     1321 tgagaaacag tttggcgatg caattcgcac caccattctg gacgttctcg cccgctacaa
     1381 cgtacgcggc gtacagctga atgtcgatga caaaggcgca ctggactgca ttttacgtgc
     1441 acgactggaa gccctgctgg cacgcgccag cggtatcccg gctctgccat gggaggattg
     1501 ccaatgattt ccgcttcgct gcaacaacgt aaaactcgca cccgccgcag catgttgttt
     1561 gtgcctggtg ccaatgccgc gatggtcagc aactcattca tttacccggc tgatgccctg
     1621 atgtttgacc tcgaagactc cgtggcattg cgtgaaaaag acaccgcccg ccgcatggtt
     1681 tatcacgcgc tgcaacatcc gctgtatcgc gatattgaaa ccattgtgcg tgtcaacgca
     1741 ctggattccg aatggggtgt taacgacctg gaagccgtcg ttcgcggtgg tgcggacgtt
     1801 gtgcgtctgc cgaaaaccga taccgctcag gatgttctgg atatcgaaaa agagatcctg
     1861 cgtatcgaaa aagcctgtgg tcgtgaaccc ggcagcaccg gtctgctggc ggcgattgaa
     1921 tctccgctgg ggattacccg cgcagtggaa atcgctcacg cttccgagcg tttgatcggt
     1981 atcgccctcg gtgcagaaga ctatgtgcgc aacctgcgta cagaacgctc cccggaagga
     2041 actgaactgc tgttcgcacg ctgttccatt ttgcaggctg cgcgctctgc gggtattcag
     2101 gcgttcgata ccgtctattc cgacgctaac aacgaagccg gattcctgca agaagccgcc
     2161 cacatcaaac agctgggctt tgacggcaaa tcgctgatca acccgcgtca gattgatctg
     2221 ctgcacaacc tctacgcacc gacccagaaa gaagtggatc acgcccgccg cgtcgtagaa
     2281 gccgctgaag ccgccgctcg cgaaggcctc ggcgtggttt ccctgaacgg caagatggtg
     2341 gacggtccgg ttatcgatcg cgcccgtctg gtgctctccc gtgcagaact ttccggcatt
     2401 cgcgaagaat aaggcaatca aaatgacgca gaaaattgaa caatctcaac gacaagaacg
     2461 ggtagcggcc tggaatcgtc gcgctgaatg cgatcttgcc gctttccaaa actcgccaaa
     2521 gcaaacctac caggctgaaa aagcgcgcga tcgcaaactg tgcgccaacc tggaagaagc
     2581 gattcgtcgc tctggtttac agaacggcat gacggtttcc ttccatcacg ctttccgtgg
     2641 cggtgacctg accgtcaata tggtgatgga cgtcatcgcg aagatgggct ttaaaaacct
     2701 gaccctggcg tccagctccc tgagtgattg ccatgcgccg ttggtagaac acattcgcca
     2761 gggcgtggtt acccgcattt atacctccgg cctgcgcggt ccactggcgg aagaaatctc
     2821 ccgtggtctg ctggcggaac cggtgcaaat ccactctcac ggcggacgtg tgcatctggt
     2881 acagagcggc gaactgaata ttgatgtggc tttcctcggc gtcccgtcat gtgatgaatt
     2941 cggtaatgcc aacggctaca ccggtaaagc ctgctgcggc tccctcggct atgcaatggt
     3001 tgatgccgac aacgcaaaac aggtcgtgat gcttaccgaa gaactgctgc cttatccgca
     3061 taatccggca agcattgagc aagatcaggt tgatctgatc gtcaaagttg atcgcgttgg
     3121 cgatgctgca aaaatcggcg ctggtgcgac ccgtatgacc actaacccgc gcgaactgct
     3181 tattgcccgt agcgctgcgg atgtgattgt caactctggc tacttcaaag aaggtttctc
     3241 tatgcaaacc ggtaccggtg gcgcatcgct ggcggtaacc cgtttcctgg aagacaaaat
     3301 gcgtagccgc gatattcgcg ccgacttcgc cctcggcggt attaccgcga cgatggttga
     3361 tctgcacgaa aaaggtctga tccgcaaact gctggatgtg cagagctttg acagccacgc
     3421 tgcgcaatcg ctggcccgta accccaatca catcgaaatc agcgccaacc agtacgctaa
     3481 ctggggttcg aaaggcgcat cggttgatcg tctcgacgtg gtggtactga gcgcgctgga
     3541 aattgacacc cagttcaacg ttaacgtgct gaccggctct gacggcgtac tgcgtggtgc
     3601 ttccggtggt cactgcgata ccgcgattgc ctctgcgctt tccatcatcg tcgcgccgct
     3661 ggtacgcggt cgtattccga ctctggtgga taacgtgctg acctgcatca ccccaggctc
     3721 cagtgtcgat attctggtca cagaccacgg tatcgcagtt aacccggcac gtccggaact
     3781 ggcagaacgt ctgcaggaat cgggcattaa agtggtttcc attgagtggc tgcgcgaacg
     3841 tgcgcgtctg ctgaccggtg aaccacagcc gattgaattc accgaccgcg tcgttgccgt
     3901 tgtgcgttac cgcgatggct cggtgatcga tgttgtgcat caggtgaagg aataagccat
     3961 gctcctgctt cctgaactcg ccagccacca tgcggtgtca attcccgagc tgctcgtcag
     4021 ccgggatgaa aggcaagcac ggcaacacgc ctggctcaag cgccatcctt ttccactggt
     4081 ctcctttacc gtggttgcgc ctgggccgat taaagacagc gaggtcacac gccgaatttt
     4141 taatcatggc gtgaccgcct tgtgtgcctt agccgcaaaa cagggctggc aaattcagga
     4201 gcaggctgca ctggtttccg ccagcgggcc ggagggcatg ttaagcattg ccgccccggc
     4261 tcgcgacctc aagctcgcca ccattgagct tgaacatagt catcctctcg ggcggttatg
     4321 ggatatcgat gtcctgacgc ccgaaggcga aattctctcc cgccgcgact attcactgcc
     4381 gcctcgccgc tgcctgttgt gcgcgcaaag cgcagctgtt tgcgcgcgtg gaaaaaccca
     4441 tcaactgacc gatttactca accgcatgga ggcactgctg aacgatgtcg atgcctgcaa
     4501 cgtcaactaa aaccacaaag cttgcgacgt cattaatcga tgagtacgcc ctgctgggct
     4561 ggcgcgccat gctgactgaa gtcaatctgt caccgaaacc aggcctcgtg gatcgcatta
     4621 actgcggtgc gcacaaagat atggcgctgg aagatttcca ccgcagcgcg ctggcgattc
     4681 agggctggtt accccgtttc attgaatttg gtgcctgtag tgcagaaatg gcaccggaag
     4741 cggtactcca cggattacgc ccaattggta tggcttgcga aggtgatatg ttccgcgcca
     4801 ctgcgggcgt aaacacgcat aaaggcagca ttttttcttt agggctgcta tgtgcggcaa
     4861 ttggccgttt gcttcagctc aaccagccgg taacgccaac aaccgtttgt tctacggcgg
     4921 caagtttctg ccgtggcctg accgatcgcg aactgcgtag caataattca caactgacgg
     4981 caggtcaacg gttgtaccag cagcttggcc ttaccggcgc acgcggtgaa gccgaagcgg
     5041 gttatccact ggtgatcaac cacgccttgc cgcattacct cactctgctg gatcaggggt
     5101 tagatcctga actggcattg ctcgatacct tgctcctact gatggcgatc aacggcgata
     5161 ccaacgttgc atcgcgcggt ggcgaggggg gcctgcgctg gctacagcgc gaggcgcaaa
     5221 cattattgca aaaagggggc attcgaaccc ccgccgatct cgattatctc cggcagttcg
     5281 acagggagtg tatcgaacga aatctcagtc caggcggcag tgccgacctg ctgatcctta
     5341 cctggttttt agcacagatt taattattta agcacttgat aaattttgaa atattaattt
     5401 tcggagaacc cgtatgtctt tagcaaaaga taatatatgg aaactattgg ccccactggt
     5461 ggtgatgggc gtcatgtttc ttatccctgt ccccgacggt atgccgccgc aggcatggca
     5521 ttacttcgct gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa ttccggcaac
     5581 agcgatcagt tttattgcgg ttactatttg cgttattggc agtaattacc tgctctttga
     5641 tgccaaagaa ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga aatggggtct
     5701 ggctggtttt tccagcacca cggtatggct ggtatttggc gcatttattt ttgcattagg
     5761 gtatgaagtt tccgggttag gtcgtcgcat tgcccttttc ctggtgaaat tcatgggcaa
     5821 acgcacgctg acgttgggtt atgcgattgt cattatcgac attctgctgg caccgtttac
     5881 accgtccaac accgcgcgta ccgggggtac ggtttttccg gtcattaaaa acctgccgcc
     5941 gctgtttaaa tcattcccga acgatccgtc cgcgcgtcgt attggcggct atttgatgtg
     6001 gatgatggtc attagtacca gtctgagttc gtccatgttt gtcaccggtg cggcaccaaa
     6061 cgtgctgggt ctggagttcg tcagcaaaat tgccggtatc cagattagct ggttgcagtg
     6121 gttcctctgc ttcctgccgg ttggggttat cttgcttatc attgcgccgt ggctttccta
     6181 cgtgctgtac aaaccggaaa tcacacacag tgaagaagtg gcaacctggg cgggtgatga
     6241 actaaaaacc atgggtgcgc tgacacgcag agagtggacg ctgattggcc ttgtattgct
     6301 cagcttaggt ttgtgggtat ttggcagtga agtcattaat gctactgcgg ttggtctgct
     6361 ggcagtttcg ctaatgctgg ccctgcacgt tgtgccgtgg aaagacatta cccgctataa
     6421 cagcgcatgg aacacgctgg tcaacctggc aactctggtt gtgatggcta acggcctgac
     6481 tcgttctggt tttattgact ggttcgccgg taccatgagt acgcacctgg aaggattctc
     6541 accaaacgca acggtgattg tactggttct ggtgttctac tttgcacact acctgtttgc
     6601 cagcctgtct gcgcacaccg caaccatgct gccggttatt ctggccgtcg gtaaagggat
     6661 cccgggcgta ccaatggaac aactgtgtat cctgctggtg ctgtctatcg gtatcatggg
     6721 ctgtctgacg ccgtatgcaa ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa
     6781 atcaaaagat tactggcgtc ttggcgcaat cttcggggtg atttacatct ctatgttgct
     6841 gttggttggc tggccgattc tcgccatgtg gaactaatac gttcgccaaa cgctgaataa
     6901 atcatcacgc ccgccaggta agcccacctg gcgggctttt ttatgattta atagatagta
     6961 ttcagtgtat gtatgagttc cacacccatt atgaaagcat tctggcgtaa cgccgcgttg
     7021 ctcgcggttt ctctgcttcc cttctcttct gccaacgccg tagcgttgca ggcaaaacag
     7081 tatggcgatt ttgatcgcta tgtcctggcc ctctcctggc aaaccggatt ttgccagagt
     7141 caacacgatc gaaatcgtaa cgaacgagat gaatgtcgcc tgcaaaccga aacgaccaac
     7201 aaagctgatt ttctgaccgt acatggtctg tggccaggat tgcctaaatc ggtcgctgcc
     7261 cgtggtgttg atgaacgccg ctggatgcgc ttcggttgcg ctactcgccc aatcccgaat
     7321 ctaccagaag cgcgcgccag ccggatgtgt tcatcgccgg aaaccggatt atcactggaa
     7381 acggccgcta aactaagtga agtcatgcct ggagctggcg gacgttcctg cctggaacgc
     7441 tacgaatatg ccaaacacgg tgcctgcttt ggttttgatc cggacgcata cttcggtacg
     7501 atggtacgcc tgaatcaaga aataaaagag agcgaagccg gaaaattcct tgcggataat
     7561 tacggtaaaa cagtgaaccg ccgtgacttt gacgccgcct ttgccaaaag ctggggaaaa
     7621 gagaacgtga aagcagttaa gctaacgtgc cagggtaacc ctgcgtattt gactgaaatt
     7681 cagatctcga tcaaagctga cgccatcaac gctccgcttt ctgcaaactc attcttgcca
     7741 caacctcacc caggcaactg tggcaaaacc tttgtgattg ataaagcggg ttattaatcc
     7801 tctgatttac aaaacacgct aattcgtctt ccataaccat gacgattaag aattatacat
     7861 aatgcgctgc attgaggaat tccctgaatg tgacgcaaat cacttcaaag agtgagcaac
     7921 agtcagtatc atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg
     7981 tatgtttatc tgcgacactc gcagtaccga cgacatggag taaaaatgtc cagaccaact
     8041 atcatcatta acgacctgga tgccgaacgc atcgatattc tgctggagca acccgcctat
     8101 gctggtttgc caatcgccga cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg
     8161 ccagaagaga tgccacacga cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt
     8221 agcgatggcg aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac cgatagcaat
     8281 actcagcttt ccgttatggc tccggtaggt gccgcactgc tggggctgcg cgttggcgat
     8341 tccattcact gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa
     8401 taccagccag aagctgctgg cgactatctg ctttaatccc atctgcacaa gcgataactc
     8461 gctttgccca gaggatgcac tcgcatcctc tttacgcaaa atcagcaatt tttcctggtt
     8521 ctcactgttc acttttgctg caaaattatt ccgtttcgtt tgtgggaata accatggcag
     8581 aagcatttta catcctgata ggatttctga ttatggcggc gattatcgtc atggctgtcc
     8641 tttacctcga aaaccactcc tgacgatcat attgttcccc ccggcatcgc attcaccaga
     8701 cctgaaaccg ccttctgcgc cgcctctgcg ctttgtgcac cgggtaccag aatcacctta
     8761 cggcactcct cttcacgttt ttcgaaaatc tcatatcccc gggcggcctc ttcaaacggc
     8821 atatagtggg taacaatttc ttctggtttc agtaatcctt tctcaattaa cggtaataat
     8881 tctcccagcc atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc tttatcaaag
     8941 gcgtcgccaa acaggaaacc gtgaataaat ccagcgtaga cgcccggtac gctaacaatg
     9001 ccgccacgcc tgaccgccgc aatacactgg cgcaacgctt taccgctgct gccctccagt
     9061 ttcaggttag tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc gacggcgtct
     9121 attactgcat ccacgccccg gtgacctgcc gtttgttcaa taattgactg tgccggatcg
     9181 ctatcttcat cgaaattaat cgggatcgcg ccgtagcggt cagcggcgaa acgcaagcgg
     9241 tagggatgat gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc acaggcgatt
     9301 gtcaacaatc ccacaggacc ggcaccatag acagcaacgc ttgaaccttg ttggatctgc
     9361 gcattttttg ctgcctgcca tgccgttggc agaatatcag aaaggaaaag ggctttatca
     9421 tctgaaagca aaggcggtac tttaaacggc cccacattcc ctttagggac gcggatatat
     9481 tccgcctgcc caccaggaac gccgccatac aggtgactat aaccaaacaa tgccgcgggt
     9541 gctggtatct gttttttatt gagcgcagcg cctttacccg cattggtatt ttcgcaggcg
     9601 gcatattgct gtaatcgaca gaaaaaacag tcgccacaag caatgacgaa cggaattacc
     9661 acccggtcgc ctttttgcaa attttttacg tccttcccgg tttcaacgac ttcccccata
     9721 aattcatgac caaaaatatc gccatgttta acctggggta ttttgcctcg ataaagatgg
     9781 aggtcagagc cacagatcgc cgttgccgta atacgcagaa taatatcatc tgcctgttca
     9841 atgcccggat cgggaacatt ttctacctga acgtgatgtg ggccgtgata agtcaatgct
     9901 ttcatgcaac ctccatagac agttcaaaaa agagtcagtc tttaagggta gctatcacag
     9961 cgggaaaacg ggcagtacca caggttttgg gagaattcct ggtgttcagt gagaatatat
    10021 tcatcacaga aacagttcgt aaggaaaaag aaccagacaa atatcaggga taaatattat
    10081 aatcatggcg aatgatttgt tcatgattaa cagggagaaa gggttatgta taagacaatc
    10141 attatgccag ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg ccacgctgaa
    10201 ttcctcgccc aggatgacgg agttattcat ctacttcacg tactacccgg gtcagccagc
    10261 ctgagcctgc accgttttgc cgctgatgtg cgtcgttttg aagagcatct gcaacatgaa
    10321 gcagaagaac gtctgcaaac gatggtcagc cacttcacca tcgatccttc ccgcattaaa
    10381 caacatgtcc gttttggtag cgtgcgggat gaagtcaatg agttggcaga agaactgggg
    10441 gctgatgttg tagttattgg ttctcgcaac ccatcgattt cgacccatct gttaggttct
    10501 aacgcctcga gcgtgatccg ccacgccaat ctgccggtgc tggttgtgcg ttaattgcca
    10561 gaataagtct ccagccctgc ccggaggcat tccggcgggg ctttttaatg ccataaaaaa
    10621 gccgccaggt ttgaccccgg cggctaagca attgcaggtg aatcttactt cttcttatgc
    10681 agttttggtg cgaatcaggt agtcaaaagc actcagagag gctttggcac cttcgccagt
    10741 ggcgatgatg atctgcttgt acggaaccgt cgtacagtca cccgctgcga acacgccttt
    10801 gacgttggtt tcgcatttcg catcaatgat aatctcgccc atgcggttac gttcgactgc
    10861 gccttccagc cagttggtgt tcggcagcag gccaatctgg acgaaaatac cagccagttc
    10921 gatgttgtga atatcgccgc tgacacgatc acgatattcc agaccaacga ctttgctgcc
    10981 gtcgcctttc acttccgtag tttgcgcatt cagaatgatg tcgacgtttt tcaggctgcg
    11041 cagtttgtcc tgcagaacct ggtcggcttt catttctggc gcaaattcca gcagcgttac
    11101 gtgctcaacg atacccgcca ggtcgattgc cgcttccacg ccggagttac cgccgccgat
    11161 aaccgctacg cgtttacctt taaacagcgg accgtcgcag tgcgggcagt aggtcacgcc
    11221 tttggtgcga tactgatctt cgcccggaac gttcatgttg cgccattttg caccggtcgc
    11281 cacgataatg ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct gatgcagacc
    11341 accttcaact gctgctggga tcagtttgct ggcgctctgg ctgtcgatca catcaacgtc
    11401 gtattcatca acgtgaactt tcagtgcgcc tgccaacttc tgcccttcag tcttcggtac
    11461 agaaatgtag ttttcgatat caacggtatc gaggatctga ccaccaaaac gttcgcccat
    11521 cagaccggta cggatgcctt tacgtgcgga gtaaattgct gccgctgcac ccgccgggcc
    11581 ggaaccaacg attaatacgt cataagcatc acgcttgttc agctcttctg ccgcacgttt
    11641 ttccgcgcca gtatcaattt tggcaacgat ttcagtcaac gtcatgcggc cctgaccaaa
    11701 ctctttcccg tttacgaaca ctgccggaac gcccatcacg ttgcgatcgg tgatttcgtt
    11761 ctggaaggtg ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcaata cgctcatcag
    11821 gttcagcgcc tgcaccacgt ccgggcagtt atggcaagag agcgagtaat aggtttcgaa
    11881 ttcaaaatca ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt ctttcgacgg
    11941 atgaccaccg gtccacagta acgccagtac cagcgaggtg aactcgtggc ccagcgggga
    12001 gcctgcaaaa cgtggcccct ggttggaacc tgggttggtg atcaggaaag acggcttacg
    12061 caccggcaag ctgttatctt ctttaaaggt gactttgtct gacagttctg cgatttcagc
    12121 caacagttcc ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa ttaactcaac
    12181 aggcttggtc aatttctcaa ggtaagcctt gagttgagtt ttcatatttg tgtcgagcat
    12241 gtttatctcc tgggctttaa acatcatcat gcaagcggcc tctgcggcta cctggatgca
    12301 gcttgcatca tggtgcgggg aagaaaatcg ggtgccgcag cacccgatta attacgcgat
    12361 tgcggaactt agattttacc aaccaggtcc agagacggag ccagagttgc ttcaccttct
    12421 ttccatttag ccgggcaaac ttcacctggg tgagaagcta cgtactgtgc tgctttgatt
    12481 ttacgcagca ggtcagacgc gtcacggcca atgccttcag cggtaacttc gattgcctgg
    12541 atgataccct gcgggtcaac aacgaaggtc gcacggtcag ccagaccttc atcttcacgc
    12601 atgttgtcga agttacgggt cagggcgcca gtcgggtcgc cgatcatcgc atatttgatt
    12661 ttagcgatgg tttcagagct gctgtgccat gctttgtggg tgaagtgagt atcggtagat
    12721 actgcgtata cgtctacgcc cagtttttgc agttcttcgt agtggtcagc aacgtcaccc
    12781 agttcggtcg ggcatacgaa agtaaagtca gccgggtaga agaagaagac gctccagcgg
    12841 ccttcggtat ctttttcggt gatttcgatg aattcgccgt ttttgaatgc ctggttttta
    12901 aaaggtttaa ttttggtgtt aatcaaggac atctatactt cctccgtgtt ttcgatgaga
    12961 tgtaaggtaa ccaatttttg ccgattcggc taatgcgttt ccattatcaa atcgataagt
    13021 tttaccttac aaccttcgta agacaacttt gtgaactttg caccgccagc ggcgataaaa
    13081 agtaaaatgg ccccctttcc aggtgaccgt gtaatctgaa ctaccctttg gtatcttcag
    13141 cgcctgcctg caggctgagg tgttgccctt acaaatgcaa caacgacatg gattacaaca
    13201 ccctcataaa caaagggcaa tcacctgatc taagctctta cctatgacag tgataggtta
    13261 tgccttttac tcgacttttg cactgactga aaaggacaaa ttaatgttaa aaaagatact
    13321 tttactggct ctgcttcctg caatcgcctt cgcagaggaa cttcctgctc cagtaaaagc
    13381 gattgaaaaa cagggtatta cgatcatcaa aacgttcgat gcccccggag gaatgaaagg
    13441 ttatctcgga aagtatcagg atatgggcgt caccatctac ctgactccag atggtaagca
    13501 cgctatctct ggttacatgt acaacgagaa aggtgaaaac ctgagtaaca cacttatcga
    13561 aaaagaaatt tacgcaccag ccggacgcga aatgtggcaa cggatggaac aatcccactg
    13621 gctcctcgac ggtaaaaaag acgcgccggt cattgtctac gtcttcgccg atccgttctg
    13681 cccatattgt aaacagttct ggcagcaggc gcgcccgtgg gtagattctg gcaaagtaca
    13741 gttgagaaca ttgttggttg gggttatcaa accagaaagt ccggcgacag ccgcggcaat
    13801 tcttgcctcc aaagatcccg caaaaacctg gcaacaatat gaagcctctg gtggcaagct
    13861 taagctaaac gtgcctgcaa acgtaagtac agagcaaatg aaagtgttaa gtgacaatga
    13921 gaaactgatg gacgatctgg gggcaaatgt cactccggct atctattaca tgagtaagga
    13981 aaatacgctg caacaggccg tggggttgcc cgatcagaaa acgcttaata tcattatggg
    14041 aaataaataa aaggtaattc atggatggtt gaactatatc cctgctttca accatcctaa
    14101 aaaagcatga attgctcgct ctgccctaag tggctgtgct attttagaaa aacttaatcc
    14161 tgagttaagc aaaaaaacat aaccaataaa aatatatacg caaaaaaaca ttgattaagt
    14221 gaatatatca tggaagaaaa atataaccgg agtagtgtat gaccaatctc tacgacttga
    14281 aaaagttcga cttaaatctt cttgtcatat ttgagtgtat ttaccagcat ttaagcatta
    14341 gcaaagctgc tgaatcacta tatatcaccc catcagccgt gagccagtcg ttacaacgct
    14401 tacgtgcgca atttaatgac cctttattca ttcgctcagg aaaaggtatc gcgccgacga
    14461 caacagggtt aaatctgcat caccatcttg agaaaaatct cagaggcctt gaacaaacaa
    14521 ttaatatagt caataagtcc gaactaaaga aaaactttat tattcacggc cctcagctca
    14581 tttcttgctc taataatagt atgctgatcc gctgcctacg acaagattcc tctgtcgaga
    14641 ttgagtgcca tgatatcctc atgtcagcag agaatgctga agaactgttg gttcaccgca
    14701 aagccgatct ggtgatcacc caaatgccag tgatcagccg ttccgtcatt tgcatgccat
    14761 tgcatacgat tcgcaatact ctaatttgta gtaataggca tccacgaatt actgacaata
    14821 gcacttatga acaaattatg gcagaagagt tcacccaatt aatatctaaa tcagcagcgg
    14881 ttgatgatat tcaaatggaa atagacgaga gatttatgaa tcgcaaaatt tcatttcgtg
    14941 gttcctcttt gctgacaatc attaatagca ttgcagttac agatttac
//
LOCUS       DAZUCE010000117        14835 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZUCE010000117 DAZUCE010000000
VERSION     DAZUCE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.117"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..122)
                     /locus_tag="ACHY03_003052"
     CDS             complement(<1..122)
                     /locus_tag="ACHY03_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFS3906253.1"
                     /translation="MDQVVIFKQIFDKVRNDLNYQWFYSELKRHNVSHYIYYLAT"
     gene            complement(180..413)
                     /locus_tag="ACHY03_003053"
     CDS             complement(180..413)
                     /locus_tag="ACHY03_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS3906254.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLCSEKRVSSEANKEKSDITELLRKQVRPD"
     gene            802..1347
                     /gene="nohD"
                     /locus_tag="ACHY03_003054"
     CDS             802..1347
                     /gene="nohD"
                     /locus_tag="ACHY03_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFS3906255.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1322..3247
                     /locus_tag="ACHY03_003055"
     CDS             1322..3247
                     /locus_tag="ACHY03_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFS3906256.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYVREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLQRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVANMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3244..3450
                     /locus_tag="ACHY03_003056"
     CDS             3244..3450
                     /locus_tag="ACHY03_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFS3906257.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3447..5048
                     /locus_tag="ACHY03_003057"
     CDS             3447..5048
                     /locus_tag="ACHY03_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS3906258.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5029..6348
                     /locus_tag="ACHY03_003058"
     CDS             5029..6348
                     /locus_tag="ACHY03_003058"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFS3906259.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEAPATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6358..6690
                     /locus_tag="ACHY03_003059"
     CDS             6358..6690
                     /locus_tag="ACHY03_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS3906260.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLMFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6746..7771
                     /locus_tag="ACHY03_003060"
     CDS             6746..7771
                     /locus_tag="ACHY03_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS3906261.1"
                     /translation="MSMYTTAQLLAANEKKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            7813..8208
                     /locus_tag="ACHY03_003061"
     CDS             7813..8208
                     /locus_tag="ACHY03_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS3906262.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVSAEMTERGLARMQ"
     gene            8220..8573
                     /locus_tag="ACHY03_003062"
     CDS             8220..8573
                     /locus_tag="ACHY03_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS3906263.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8585..9163
                     /locus_tag="ACHY03_003063"
     CDS             8585..9163
                     /locus_tag="ACHY03_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3906264.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9160..9555
                     /locus_tag="ACHY03_003064"
     CDS             9160..9555
                     /locus_tag="ACHY03_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFS3906265.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VTSGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            9563..10303
                     /locus_tag="ACHY03_003065"
     CDS             9563..10303
                     /locus_tag="ACHY03_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3906266.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            10319..10741
                     /locus_tag="ACHY03_003066"
     CDS             10319..10741
                     /locus_tag="ACHY03_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFS3906267.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            10723..11157
                     /locus_tag="ACHY03_003067"
     CDS             10723..11157
                     /locus_tag="ACHY03_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFS3906268.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11150..13729
                     /locus_tag="ACHY03_003068"
     CDS             11150..13729
                     /locus_tag="ACHY03_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS3906269.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDLLRGNWMAGLRSGWSEWKESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGINFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQAQMRDGGLFSG
                     GGR"
     gene            13726..14055
                     /locus_tag="ACHY03_003069"
     CDS             13726..14055
                     /locus_tag="ACHY03_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3906270.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14055..14753
                     /locus_tag="ACHY03_003070"
     CDS             14055..14753
                     /locus_tag="ACHY03_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS3906271.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTEMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14759..>14835
                     /locus_tag="ACHY03_003071"
     CDS             14759..>14835
                     /locus_tag="ACHY03_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3906272.1"
                     /translation="MTETESAILAHARRCAPAESCGFVV"
BASE COUNT         3384 a         3734 c         4622 g         3095 t
ORIGIN      
        1 gtggctaaat agtaaatgta atgtgagaca ttgtgacgtt ttagctcaga ataaaaccat
       61 tgatagttta aatcgtttcg aactttatca aatatttgtt taaaaatgac tacctgatcc
      121 atagataaac cttccatgtg atatgagggg gcgtagtctg cacgattatc taaattgctt
      181 caatctggtc tgacctgttt tctgagcaat tcagtaatgt cactcttttc tttgtttgct
      241 tcagaagaaa ctcttttttc tgagcacagt ctccggcggc aggcttcaat gacccaggct
      301 gagaaattcc cggacccttt ttgctcaaga gcgatgttaa tttgttcaat catttggtta
      361 ggaaagcgga tgttgcgggt tgttgttctg cgggttctgt tcttcgttga catgaggttg
      421 tcccgtattc agtgtcgctg atttgtattg tctgaagttg tttttacgtt aagttgatgc
      481 agatcaatta atacgatatc tgcgtcataa ttgattattt gacgtggttt gatggcgtag
      541 atgcacgttg tgacatgcag atgataatta ttatcatttt gcgggtcctt tccggcgatc
      601 cgacaggtta cggggcggcg acctcgcggg ttttcgctat ttatgaaaat tttccggttt
      661 aaggcgtttc cgttcttctt cgttgtaact taatgttttt atttaaaata ccccctgaaa
      721 agaaaggaaa cgacaggtgc tgaaaacgag cttttgggcc tctgtcgttt cctttctctg
      781 tttttggccg tggaatgaac aatggaagtc aacaaaaagc agctggctga cattttcggt
      841 gcgagtatcc gtaccattca gaactggcag gaacagggaa tgcccgttct gcgaggcggt
      901 ggcaagggta atgaggtgct ttatgactct gccgccgtta taagatggta tgccgaaagg
      961 gatgctgaaa ttgagaacga aaagctgcgc cgggaagttg aagaactgcg gcaggccagc
     1021 gaggcagatc tccagccagg gactattgag tacgaacgcc atcgacttac gcgtgcgcag
     1081 gccgacgcac aggaactgaa gaatgccaga gactccgctg aagtggtgga aaccgcattc
     1141 tgtactttcg tgctgtcgcg gatcgcaggt gaaattgcca gtattctcga cgggatcccc
     1201 ctgtcggtgc agcggcgttt tccggaactg gaaaaccgac atgttgattt cctgaaacgg
     1261 gatatcatca aagccatgaa caaagcagcc gcgctggatg aactgatacc ggggttgctg
     1321 agtgaatata tcgaacagtc aggttaacag gctgcggcat tttgtccgcg ccgggcttcg
     1381 ctcactgttc cggccggagc cacagaccgc cgttgaatgg gcggatgcca attactatct
     1441 cccgaaagaa tccgcatacc aggaagggcg ctgggaaaca ctgccctttc agcgggccat
     1501 catgaatgcg atgggcagtg actacgtccg cgaggtgaat gtggtgaagt ctgcccgtgt
     1561 tggttattcc aaaatgctgc tgggtgttta tgcctacttc atagagcata agcagcgcaa
     1621 cacccttatc tggttgccga cggatggtga tgccgagaac tttatgaaaa cccacgttga
     1681 gccgaccatc cgcgatattc cgtcgctgct ggcgctggct ccgtggtatg gcaaaaagca
     1741 ccgggataac acgctcacca tgaagcgttt ttccaatggt cgtggcttct ggtgcctggg
     1801 cggtaaagcg gcaaaaaact accgtgaaaa gtcggtggat gtggcgggtt atgatgaact
     1861 tgctgccttt gatgaggata ttgaacagga aggctctccg acgttcctgg gcgataagcg
     1921 tattgaaggc tcggtctggc caaagtccat ccgtggctcc acgcccaaag tgagaggcac
     1981 ctgccagatt gagcgtgcag ccagtgaatc cccgcatttt atgcgttttc atgttgcctg
     2041 cccgcactgc ggggaggagc agtaccttaa atttggcgat aaagagacgc cgtttggcct
     2101 caaatggacg ccggatgacc cctccagcgt gttttatctc tgcgagcata acgcctgcgt
     2161 catccgtcag caggagctgg acttcactga tgcccgttat atctgcgaaa agaccgggat
     2221 ctggacccgt gatggcattc tctggttttc gtcatccggt gaagagattg aaccgcctga
     2281 cagcgtgacc tttcacatct ggacggcgta cagcccgttc accacctggg tgcagattgt
     2341 caaagactgg atgaagacga aaggggatac gggaaaacgt aaaaccttcg tgaacaccac
     2401 gctcggtgag acgtgggaag cgaaaatcgg tgaacgtccg gatgctgaag tgatggcaga
     2461 gcggaaagag cattattcag cgcccgttcc tgaccgtgtg gcttacctga ccgccggtat
     2521 cgactcccag ctggatcgct acgaaatgcg cgtttgggga tgggggccgg gtgaggaaag
     2581 ctggctgatt gaccggcaga ttattatggg ccgccacgac gatgaacaga cgctgcagcg
     2641 tgtggatgag gccatcaata aaacctatac ccgccggaat ggtgcagaaa tgtcggtatc
     2701 ccgtatctgc tgggatactg gcgggattga ccctaccatt gtgtatgaac gctcgaaaaa
     2761 acatgggctg ttccgggtga tccccattaa aggggcatcc gtctacggta agccggtggc
     2821 caacatgcca cgtaagcgaa acaaaaacgg ggtttacctt accgaaatcg gtacggatac
     2881 cgcgaaagag cagatttata accgcttcac actgacgccg gaaggggatg aaccgcttcc
     2941 cggtgccgtt cacttcccga ataacccgga tatttttgat ctgaccgaag cgcagcagct
     3001 gactgctgaa gagcaggtcg aaaaatgggt ggatggcaga aaaaaaatac tgtgggacag
     3061 caaaaagcga cgcaatgagg cgctcgactg cttcgtttat gcgctggcgg cgctgcgcat
     3121 cagtatttcc cgctggcagc tggatctcag tgcactgctg gcgagcctgc aggaagagga
     3181 tggtgcagca accaacaaga aaacactggc agattacgcc cgtgccttat ccggagagga
     3241 tgaatgacgc gacaggaaga acttgccgct gcccgtgcgg cactgcatga cctgatgaca
     3301 ggtaaacggg tggcaacggt acagaaagac ggacggcgag tggagtttac ggccacttcc
     3361 gtgtctgacc tgaaaaaata cattgcggag ctggaagtgc agaccggcat gacacagcga
     3421 cgcaggggac ctgcaggatt ttatgtatga aaacgtccac cattcccacc cttctggggc
     3481 cggacggcat gacatcgctg cgtgaatatg ccggttatca cggcggtggc agcggatttg
     3541 gtgggcagtt gcgggcgtgg aacccaccgg gtgaaagtgt ggatgcagcc ctgctgccca
     3601 attttacccg tggcaatgcc cgtgcagacg atctggtacg caataacggc tatgccgcca
     3661 acgccatcca gctgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc
     3721 caagctggcg ctatctgggc atcggggagg aagacgcccg tgccttttcc cgcgaggttg
     3781 aagcggcatg gaaagagttt gccgaagatg actgttgctg cattgacgtt gagcgaaaac
     3841 gcacgtttac catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgaactgt
     3901 tcgttcaggc cacctgggat accagtccgt cgcggctttt ccggacacag ttccggatgg
     3961 tcagcccgaa gcgcatcagc aacccgaaca ataccggcga cagccggaac tgccgtgccg
     4021 gtgtgcagat taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc
     4081 ctggctggat gccgcagaaa tggacatgga taccccgtga gttacccggc gggcgcgcct
     4141 cgttcattca cgtttttgaa cccgtggagg acgggcagac ccgcggtgca aatgtgtttt
     4201 acagcgtaat ggagcagatg aagatgctcg acacgctgca gaacacgcag ctgcagagcg
     4261 ccattgtgaa ggcgatgtat gccgccacca ttgaaagtga gctggatacg cagtcagcga
     4321 tggattttat tctgggcgcg aacagtcagg agcagcggga aaggctgacg ggctggattg
     4381 gtgaaattgc cgcgtattac gccgcagcac cggtccgtct gggaggcgca aaagtgccgc
     4441 acctgatgcc gggggactca ctgaacctgc agacggctca ggacacggat aacggctact
     4501 ccgtgtttga gcagtcactg ctgcggtata tcgctgccgg gctgggtgtc tcgtatgagc
     4561 agctttcccg gaattacgcc cagatgagct actccacggc acgggccagt gcgaacgagt
     4621 cgtgggcgta ctttatgggg cggcgaaaat tcgtcgcatc ccgtcaggcg agccagatgt
     4681 ttctgtgctg gctggaagag gccatcgttc gccgcgtagt gacgttacct tcaaaagcgc
     4741 gcttcagctt tcaggaagcc cgcagtgcct gggggaactg cgactggata ggctccggtc
     4801 gtatggccat cgatggtctg aaagaagttc aggaagcggt gatgctgata gaagccggac
     4861 tgagcaccta cgagaaagag tgcgcgaaac gcggtgacga ctatcaggaa atttttgccc
     4921 agcaggtccg tgaaacgatg gagcgccgcg cggctggtct taaaccgccc gcctgggcgg
     4981 ctgcagcatt tgaatccggg ctgcgacaat caacagagga ggagaagagt gacagcagag
     5041 ctgcgtaatc tcccgcatat tgccagcatg gcctttaatg agccgctgat gcttgaaccc
     5101 gcctatgcgc gggttttctt ttgtgcgctt gcaggccagc ttgggatcag ccgcctgacg
     5161 gatgcggtgt ccggcgacag cctgactgcc caggaggcac ccgcgacgct ggcattatcc
     5221 ggtgatgatg acggaccacg acaggcccgc agttatcagg tcatgaacgg catcgccgtg
     5281 ctgccggtgt ccggcacgct ggtcagccgg acgcgggcgc tgcagccgta ctcggggatg
     5341 accggttaca acggcattat cgcccgtctg caacaggctg ccagcgaccc gatggtggac
     5401 ggcattctgc tcgatatgga cacaccgggc ggaatggtgg cgggagcatt tgactgcgct
     5461 gacatcatcg cccgtgtgcg tgacataaag ccggtatggg cgctggccaa cgacatgaac
     5521 tgcagtgcag gtcagctgct tgctagtgcc gcctcccggc gtctggtcac gcagaccgcc
     5581 cggacaggct ccatcggcgt catgatggct cacagtaatt acggcgctgc gctggagaaa
     5641 cagggcgtgg aaatcacgct gatttacagc ggcagccata aggtggatgg caacccctac
     5701 agccatcttc cggatgacgt ccgggagaca ctgcagtccc ggatggatgc aacccgccgg
     5761 atgtttgcgc agaaggtgtc ggcatatacc ggcctgtccg tgcaggctgt gctggatacc
     5821 gaggctgcag tgtacagcgg tcaggaggcc attgatgccg gactggctga tgaacttgtc
     5881 aacagcaccg atgcgatcac cgttatgcgt gatgcactgg atgcacgtaa atcccgtctc
     5941 tcaggagggc gaatgaccaa agagactcaa tcaacaactg tttcagccac tgcttcgcag
     6001 gctgacgtta ctgacgtggt gccagcgacg gagggcgaaa acgccagcgc ggcgcagccg
     6061 gacgtgaacg cgcagatcac cgctgcggtt gcggcagaaa acagccgcat tatggggatc
     6121 ctcaactgtg aggaggctca cggacgcgaa gaacaggccc gcgtgctggc agaaaccccc
     6181 ggtatgaccg tggaaacggc ccgccgcatt ctggccgcag caccacagag tgcacaggcg
     6241 cgcagtgaca ctgcgctgga tcgtctgatg cagggggcac cggcaccgct ggctgcaggt
     6301 aacccggcat ctgatgccgt taacgatttg ctgaacacac cagtgtaagg gatgtttatg
     6361 acgagcaaag aaacctttac ccattaccag ccgctgggca acagtgaccc ggctcatacc
     6421 gcaaccgcgc ccggcggatt gagtgcgaaa gcgcctgcaa tgaccccgct gatgctggac
     6481 acctccaccc gtaagctggt tgcgtgggat ggcaccaccg acggtgctgc cgttggcatt
     6541 ctggcggttg ctgctgacca gaccagcacc acactgatgt tctacaagtc cggcacgttc
     6601 cgttatgagg atgtgctctg gccggaggct gccagcgacg agacgaaaaa acggaccgcg
     6661 tttgccggaa cggcaatcag catcgtttaa ccttaccctt catcactaaa ggccgcctgt
     6721 gcggcttttt ttatgggatt tttttatgtc gatgtacaca accgcccagc tgctggcggc
     6781 aaatgagaag aaatttaagt ttgatccgct gtttctgcgt ctctttttcc gtgagagcta
     6841 tcccttcacc acggagaaag tctatctctc acaaattccg ggactggtaa acatggcgct
     6901 gtacgtttcg ccgattgttt ccggtgaggt tatccgctcc cgtggcggct ccacctctga
     6961 atttacaccg ggatatgtca agccgaagca tgaggtgaat ccgcagatga ccctgcgtcg
     7021 cctgccggat gaagatccgc agaatctggc ggacccggct taccgccgcc gtcgcatcat
     7081 catgcagaac atgcgagacg aagagctggc cattgctcag gtcgaagaga tgcaggcagt
     7141 ttctgctgtg cttaagggca aatacaccat gaccggtgaa gccttcgatc cggttgaggt
     7201 ggatatgggc cgcagtgcgg cgaacaacat cacacagtcc ggtggtacgg agtggagcaa
     7261 gcgtgacaag tccacgtatg acccgaccga cgatatcgaa gcctatgcgc tgaacgccag
     7321 cggagtggtg aatatcatcg tgtttgatcc gaaaggctgg gcgctgttcc gttccttcaa
     7381 agccgtcagg gagaagctgg atacccgtcg cggctctaat tccgagctgg agacagcggt
     7441 aaaagacctg ggcgaagcgg tgtcctataa ggggatgtat ggcgatacgg cgatcgtcgt
     7501 gtattccgga cagtacgtgg aaaacgacgt caaaaagaac ttccttccgg acaacacgat
     7561 ggtgctgggg aacactcagg cacgcggtct gcgcacctat ggctgcattc aggatgcgga
     7621 cgcacagcgc gaaggtatta acgcctctgc ccgctacccg aaaaactggg tgaccaccgg
     7681 cgatccggcg cgtgagttca ccatgattca gtcagcaccg ctgatgctgc tggctgaccc
     7741 tgatgcgttc gtgtccgtac aactggcgta atcatggccc ttcggggcca ttttctctct
     7801 gtggaggagt ccatgacgaa agatgaactg attgcccgtc ttcaggtgct gggtgagcaa
     7861 ctgaaccgtg atgtcagcct gacggggacg aaagaagaac tggtgctccg tgtggcagag
     7921 ctggaagagg agcttgatga cacggatgac gctgccggtc aggacacatc tgtcagcccg
     7981 gaaaatgcgc tgaccggaca tgaaaatgag gtggtatcag cgcagccgga taccgtgact
     8041 gatacggctg atctggtcac ggttgtggca ctggtgacgc tgcatactga tgcacttcac
     8101 gccacgcggg atgaggctgt ggcatttgtg ctgccgggaa cggcgttccg tgtctctgcc
     8161 ggtgtgtcag ctgaaatgac agagcgcggc ctggccagaa tgcaataacg ggaggcgctg
     8221 tggctgattt cgataacctg ttcgatgctg ccattgcccg cgccgatgaa acgatacgcg
     8281 ggtacatggg aacgtcagcc accatgacat ccggtgagca gtccggtgct gtgatacgtg
     8341 gtgtttttga tgaccctgaa aatatcagct atgccggaca gggcgtgcgc gttgaaggct
     8401 ccagcccgtc cctgtttgtc cggactgatg atgtgcggca gctgcggcgc ggcgacacgc
     8461 tgaccatcgg tgaggaaaac ttctggatag accggatttc gccagatgat ggcggaagct
     8521 gtcatctctg gcttgggcgg ggcgtaccgc ctgccgttaa ccgtcgccgc tgaaaggggg
     8581 atgtatggcc ataaaaggtc ttgagcaggc cgttgaaaac ctcagccgta tcagcagaac
     8641 ggcggtgccc ggtgccgccg caatggccat taaccgcgtt gcttcatccg cgatatcgca
     8701 gtcggcgtca caggttgccc gtgagacaaa ggtacgccgg aaactggtaa aggaaagggc
     8761 caggctgaaa agggccacgg tcaaaaatcc gcaggccaga atcaaggtta accgggggga
     8821 tttgcccgta ataaagctgg gtaacgcgcg gattgtcctg tcccgacgca ggcgtcgtaa
     8881 aaaggggcag cgttcagccc tgaaaggtgg cggcagcgtg cttgtggtgg gaaaccgtcg
     8941 tattcccggc gcgtttattc agcaactgaa aaatggccgc tggcatgtca tgcagcgtgt
     9001 ggccgggaaa aatcgttacc ccattgatgt ggtgaaaatc ccgatggcgg tgccgctgac
     9061 cacggcgttt aaacagaata ttgagcggat acggcgtgag cgtcttccga aagagctggg
     9121 ctatgcgctg cagcatcaac tgaggatggt aataaagcga tgaaacatac tgaactccga
     9181 gcagccgtac tggatgcact ggagaagcat gacaccgggg cgacgctttt tgatggtcgc
     9241 cccgctgttt ttgatgaggc ggattttccg gcaattgccg tttatctcac cggcgctgaa
     9301 tacacgggcg aagagctgga cagcgatacc tggcaggcgg agctgcatat tgaagttttc
     9361 ctgcctgctc aggtgccgga ttcagagctg gatgcgtgga tggagtcccg gatttatccg
     9421 gtgatgagtg atatcccggc actgtcagat ttgatcacca gtatggtgac cagcggctat
     9481 gactatcggc gcgacgatga tgcgggcctg tggagttcag ccgatctgac ttatgtcatt
     9541 acctatgaaa tgtgaggacg ctatgcctgt accaaatcct gtaatgccgg tgaaaggggc
     9601 cgggaccacc ctgtgggttt ataaggggag tggtgaccct tatgcgaatc cgctttcaga
     9661 cgttgactgg tcgcgtctgg caaaagttaa agacctgacg cccggcgaac tgaccgctga
     9721 gtcttatgac gacagctatc tcgatgatga agatgcagac tggactgcga ccgggcaggg
     9781 gcagaaatct gccggagata ccagcttcac gctggcgtgg atgcccggag agcaggggca
     9841 gcaggcgctg ctggcgtggt ttaatgaagg cgatacccgt gcctataaaa tccgcttccc
     9901 gaacggcacg gtcgatgtgt tccgtggctg ggtcagcagt atcggtaagg cggtgacggc
     9961 gaaggaagtg attacccgca cggtgaaggt caccaatgtg ggacgtccgt cgatggcaga
    10021 agatcgcagc acggtgacgg cgacaaccgg catgacggtg acacccgcca gtgcttccgt
    10081 agtgaaaggg cagagcacca cgctgaccgt ggcattccag ccggatggcg caaccgacaa
    10141 gagcttccgt gcggtgtctg cggataaaac aaaagccacc gtgtcggtca gtggtatgac
    10201 catcaccgtg aaaggtgttg ctgcaggcaa ggtcaacatt ccggtcgtat ccggtaatgg
    10261 tgagtttgct gcggttgcag aaattaccgt caccgccagt taatccggag agtcagcgat
    10321 gttcctgaaa accgaatcat ttgaacataa cggtgtgacc gtcacgcttt ctgaactgtc
    10381 agccctgcag cgcattgagc atctcgccct gatgaaacgg caggcagaac aggcggagtc
    10441 agacagcaac cggaagttta ctgtggaaga cgccatcaga accggcgcgt ttctggtggc
    10501 gatgtccctg tggcataacc atccgcagaa gacgcagatg ccgtccatga atgaagccgt
    10561 taaacagatt gagcaggaag tgcttaccac ctggcccacg gaggcaattt ctcatgctga
    10621 aaacgtggtg taccggctgt ccggtatgta tgagtttgtt gtgaatgatg ctcctgaaca
    10681 ggcagaggac gccgggcctg cagagcctgt ttctgcggga aagtgttcga cggtgagctg
    10741 agttttgccc tgaaactggc gcgtgagatg gggcgacccg actggcgcgc catgcttgcc
    10801 gggatgtcat ccacggagta tgccgactgg caccgctttt acagtaccca ttattttcat
    10861 gatgttctgc tggatatgca cttttccggg ctgacgtaca ccgtgctcag cctgtttttc
    10921 agcgatccgg agatgcatcc gctggatttc agtctgctga accggcgcga ggctgacgaa
    10981 gagcctgaag atgatgtgct gatgcagaaa gcggcagggc ttgccggagg cgttcgtttt
    11041 ggcccggacg ggaatgaagt tatccccgct tccccggatg tggcggacat gacggaggat
    11101 gacgtaatgc tgatgacagt atcagaaggg atcgcaggag gagtccggta tggctgaacc
    11161 ggtaggcgat ctggtcgttg atttaagtct ggatgcggcc agatttgacg agcagatggc
    11221 cagagtcagg cgtcattttt ccggtacgga aactgatgcg aaaaaaacag cggcagtcgt
    11281 tgaacagtcg ctgagccgac aggcgctggc tgcacagaaa gcggggattt ccgtcgggca
    11341 gtataaagcc gccatgcgta tgctgcctgc acagttcacc gacgtggcca cgcagcttgc
    11401 aggcgggcaa agcccgtggc tgatcctgct gcaacagggt ggtcaggtta aggactcctt
    11461 cggcgggatg atccccatgt tcagggggct tgccggtgcg atcaccctgc cgatggtggg
    11521 ggccacctcg ctggcggtgg cgaccggtgc gctggcgtat gcctggtatc agggcaactc
    11581 aaccctgtcc gatttcaaca aaacgttggt cctttccggc aatcaggcgg gactgacggc
    11641 agatcgtatg ctggtcctgt ccagagccgg gcaggcggca gggctgacgt ttaaccagac
    11701 cagcgagtca ctgacggcgc tggtgaatgc cggtgtgcgt ggtggtgagc agtttgaggc
    11761 gatcagccag agtgtggcgc gtttctcctc tgcatccggc gtggaggtgg acaaggtcgc
    11821 tgaagccttc gggaagctga ccacagaccc gacgtcggga ctgacagcga tggcacgtca
    11881 gttccataac gtgacggcgg agcagattgc gtatgttgct cagttgcagc gttccggaga
    11941 tgaagccggg gcattgcagg cggcgaacga ggccgcaacg aaagggtttg atgaccagac
    12001 ccgccgcctg aaagagaaca tgggcacgct ggagacctgg gcagacagga cagcacgggc
    12061 attcaaatcc atgtgggatt cggtgctgga tattggtcgc ccggacactg cccagggaat
    12121 gctggagaaa gcagaaaagg cttttgatga ggcggacaaa aaatggcagt ggtatcagag
    12181 ccggagccac cggcgcggta aaacctcagc atttcttgcc aatctccggg gagcatggga
    12241 ggacagagcg aatgcgcaac ttgggctttc agccgccacg ttgcaggccg atcttgaaaa
    12301 ggccagagag atggcagcaa aggactgggc cgagtctgag gcatcacggc tgaaatatac
    12361 cgaagaggcg cagaaggctt acgaacgcct gcagacgccg ctggagaaat ataccgcccg
    12421 tcaggaagaa ctgaacaagg cactgaagga cgggaaaatt ctgcaggcag attacaacac
    12481 gctgatggcg gcggcgaaaa aggactatga agcgacgctg aaaaagccga aacagtccgg
    12541 cgtgaaggtg tctgcgggcg atcgtcagga agacagtgct catgctgccc tgctgacgct
    12601 tcaggcagaa ctccggacgc tggagaagca tgccggagca aatgagaaaa tcagccagca
    12661 gcgccgggat ttgtggaagg cggagagtca gttcgcggta ctggaggagg cggcgcaacg
    12721 tcgccagctg tctgcacagg agaaatccct gctggcgcat aaagatgaga cgctggagta
    12781 caaacgccag ctggctgcac ttggcgacaa ggttacgtat caggagcgcc tgaacgcgct
    12841 ggcgcagcag gcggataaat tcgcacagca gcaacgggca aaacgggccg ctattgatgc
    12901 gaaaagccgg gggctgactg accggcaggc agaacgggaa gccacggaac agcgcctgaa
    12961 ggaacagtat ggcgataatc ctctggcgct gaataacgtc atgtcagagc agaaaaagac
    13021 ctgggcggct gaagacctgc ttcgcgggaa ctggatggca ggcctcaggt ccggctggag
    13081 cgagtggaaa gagagtgcca cggacagtat gtcgcaggtt aaaagtgcag ccacgcagac
    13141 ctttgatggt attgcgcaga atatggcggc gatgctgacc ggcagtgaac agaactggcg
    13201 cagcttcacc cgctccgtgc tgtccatgat gacagaaatt ctgcttaagc aggcaatggt
    13261 ggggattgtc gggagtatcg gcagcgccat tggcggggct gttggtggcg gcgcatccgc
    13321 gtcaggcggt acagccattc aggccgctgc ggcgaaattc cattttgcga ccgggggatt
    13381 tacgggaacc ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt
    13441 cacgaaggag gcaaccagcc ggattggtgt cggcaacctg taccgcctga tgcggggcta
    13501 tgcggaaggt ggttatgtgg gcggtgccgg aagtccggcg cagatgcggc gggctgaagg
    13561 cattaatttt aatcagaaca atcacgtggt gattcagaac gacggtacga atggtctgcc
    13621 aggtccacag atgatgaagg cggtgtatga catggcccgc aagggtgccc gtgatgaaat
    13681 ccaggcacag atgcgcgatg gtggtctgtt ctccggaggt ggacgatgaa aaccttccgc
    13741 tggaaagtga aacccggtat ggatgtggct tcggcccctt ctgtaagaaa ggtgcgcttt
    13801 ggtgatggct attcccagcg agcgcctgcc gggctgaatg ccaacctgaa aacgtacagc
    13861 gtgacgcttt ctgtcccccg tgaggaggcc acggtactgg agtcgtttct ggaagagcac
    13921 gggggctgga aagcctttct gtggacgccg ccttatgagt ggcggcagat aaaggtgacc
    13981 tgcgcaaaat ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa
    14041 caggtggtga actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg
    14101 agcagtcggc cagcgtggtg ctctgggaaa tcgacctgac agaggtcggt ggagaacgtt
    14161 attttttctg taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt
    14221 atcagccgta tcccattcag gggagtggtt ttgaactgaa tggcaaaggc accagtacgc
    14281 gccccacgct gacggtttct aacctgtacg gtatggtcac cgagatggcg gaagatctgc
    14341 agagtctggt cggcggaacg gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg
    14401 tgaacttcgt caacggaaac agcgacgccg atccggagca ggaggtgatc agccgctggc
    14461 gcatcgagca gtgcagcgaa ctgagcgcgg tcagtgcttc ctttgtgctc tccacaccga
    14521 cggaaacgga tggtgccgtt tttccgggcc gcatcatgct ggctaatacc tgcacctgga
    14581 cctatcgcgg tgatgagtgc ggttatcacg gtccggctgt cgcggatgaa tatgaccagc
    14641 cgacgtccga tatcacgaag gataaatgca gcaaatgcct gagcggttgt aagtttcgca
    14701 ataacgtcgg caactttggc ggcttccttt ccattaacaa actttcgcag taaatcccat
    14761 gacagagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
    14821 cggcttcgtg gtaag
//
LOCUS       DAZUCE010000118        14765 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZUCE010000118 DAZUCE010000000
VERSION     DAZUCE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.118"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(143..2410)
                     /gene="adiA"
                     /locus_tag="ACHY03_003072"
     CDS             complement(143..2410)
                     /gene="adiA"
                     /locus_tag="ACHY03_003072"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFS3906273.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2609..3517)
                     /gene="melR"
                     /locus_tag="ACHY03_003073"
     CDS             complement(2609..3517)
                     /gene="melR"
                     /locus_tag="ACHY03_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFS3906274.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3800..5155
                     /gene="melA"
                     /locus_tag="ACHY03_003074"
     CDS             3800..5155
                     /gene="melA"
                     /locus_tag="ACHY03_003074"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFS3906275.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5258..6679
                     /gene="melB"
                     /locus_tag="ACHY03_003075"
     CDS             5258..6679
                     /gene="melB"
                     /locus_tag="ACHY03_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFS3906276.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIAFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6818..7447)
                     /gene="yjdF"
                     /locus_tag="ACHY03_003076"
     CDS             complement(6818..7447)
                     /gene="yjdF"
                     /locus_tag="ACHY03_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFS3906277.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7570..9216)
                     /gene="fumB"
                     /locus_tag="ACHY03_003077"
     CDS             complement(7570..9216)
                     /gene="fumB"
                     /locus_tag="ACHY03_003077"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFS3906278.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9294..10634)
                     /gene="dcuB"
                     /locus_tag="ACHY03_003078"
     CDS             complement(9294..10634)
                     /gene="dcuB"
                     /locus_tag="ACHY03_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFS3906279.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11205..11924)
                     /gene="dcuR"
                     /locus_tag="ACHY03_003079"
     CDS             complement(11205..11924)
                     /gene="dcuR"
                     /locus_tag="ACHY03_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFS3906280.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11921..13552)
                     /gene="dcuS"
                     /locus_tag="ACHY03_003080"
     CDS             complement(11921..13552)
                     /gene="dcuS"
                     /locus_tag="ACHY03_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFS3906281.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13733..13963
                     /gene="yjdI"
                     /locus_tag="ACHY03_003081"
     CDS             13733..13963
                     /gene="yjdI"
                     /locus_tag="ACHY03_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFS3906282.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            13975..14247
                     /gene="yjdJ"
                     /locus_tag="ACHY03_003082"
     CDS             13975..14247
                     /gene="yjdJ"
                     /locus_tag="ACHY03_003082"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3906283.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14474..>14765
                     /gene="ghoS"
                     /locus_tag="ACHY03_003083"
     CDS             14474..>14765
                     /gene="ghoS"
                     /locus_tag="ACHY03_003083"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFS3906284.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFY"
BASE COUNT         3563 a         3528 c         3830 g         3844 t
ORIGIN      
        1 aaaaaagcgc ggggtggggc cgcgcaaaaa gtatgacatt tttgtttgca gcaattttta
       61 tgaaaacgtt gccatactca aaaggcctaa ggccaggcgg cgtaatgtta tttaaacaat
      121 tacgccttca gcggaatagt ggttacgctt tcacacacat aacgtggtaa ataccgtcaa
      181 taatttcagt cccttcagtt tcgtgttcaa atccagggaa atgatggtcc caggattgca
      241 gcgagcgtaa ataacttact tgcggactgt ttttatcgcc gaagttttca ccagacagca
      301 gcatcgggat tcctggcgga tacgggataa ctgagtttgc cgcgatgcgt cctggtagat
      361 tttcaatgga taccagttcg acattgttat cgacaatcgc gttgtacgct tcacgcgggg
      421 tgatctccgc caccggcagg ccggaatagg cttcgttcaa ccgtgcgcca ggattgtttt
      481 ctttcagcca ggcaaacatg gtgtcaccca gatcgtgaat ccccatgttc gcgtaagtgt
      541 caggatattg ttcaacaagt tccggcatca cctgcgccag cggtgtgttg gcgtcatagt
      601 ggcgtttgaa ggagcaaagg gtgttaacca gagtccccca tttcccacgg gttacgccca
      661 tagagaacag gaacataatt tggaagtcag tggtgcgggt aggcacaatg ccgtggcgac
      721 caagccaggc agtgaccagc gccgccggaa caccggtttc ttccagttca ccatcttcac
      781 ccattcccgg agcaaggatg ctgactttaa tcgggtcgag catactccag ttatccggaa
      841 tatctttgaa gccgtgccag ctttcgcccg gatgcattac ccagcagtcc tgaacggtgg
      901 tcaacagttt ggttggcgcg tcagcaaagt cataggtttt gccggtttgt gggtcggtga
      961 cgacttcttt gttccacggt ttgaagaacc agctaccgtc agcggtgaac tctttataca
     1021 gccgcgccat cgcctgacgg aaatcaaccg cttcgtcgat cacttcctgc gtcagtgaca
     1081 ggccactgtt gccgtccatc atcgacaccg ccacgtcgtt ggatgcgcag atggcataca
     1141 gcggggaggt ggtggcatgc atcatgtacg cctgattgaa gcgggagaag ttaatcgcac
     1201 cacgaccttc acgcacatga atgtaagagg cctgtgacag cgcattcagc agtttgtggg
     1261 tggagtgggt ggcgaaaacg gtagggccgt tgtgatcgcc aggttcgccg cgcatggcat
     1321 agtgatcggc atagatcggg ttgaaacgtg catagccgta ccaggcttcg tcaaagtgca
     1381 gacgatcgga ggttttttcc agcagatcct gcgcttcttt agcgttataa cacacgccgt
     1441 cataggtgca gttggtcacc acgcagtaag acggtttttg cccggctttg tctttagtca
     1501 gcgggctttc actgattttc ttctgcaagg tttcaggttg catttcctgc ggatagattg
     1561 gcccgataat gccgtagcgg ttgcggcttg gcaccatgta gaccggtttc gcgcctgtca
     1621 gcatcaaacc ttgttcgatg gatttatggc agttacggtc aacaaccact acatcgttat
     1681 cggtcatgca agcctgcatg atggtgcggt tagagccgga agtaccgacg actaccgacc
     1741 aggagcgatc ggcaccaaat acgcgtgcgg catatttttc gctttcgcca aatgcgccag
     1801 tatggtcaag caaagaaccg agggaagttc gttcgatgcc catgtcggtg cggaacagat
     1861 tttcaccata gtagtcatgg tagaaacgtc cggcgggtgt tttggtaaaa ccaacgccgc
     1921 cctggtggcc tggcgctgcc caggaatatt catggatgtc gctatatttc atcagcgcgc
     1981 tgaacagtgg cggcaacagc tgctggcggt agcgggtcat cgcggcaacg gcgcgtccgg
     2041 cgataaagtc ggcggtatct tccagaatcc aggcgaattc atcgacaagc tccagcaggt
     2101 cgcgatccat tgcggcgagg gctttttccc gatcgcccaa caggaagacc ggcacgtttt
     2161 gttggcgctc atgaagctta ccgatcaatt gtctgacgtt ttgatgttcg tccggatgtt
     2221 ccatttgata gctgaacatc aggcagtcaa tggcttcgtt tgaagagaga atggcaaaac
     2281 catcatcaaa ggaggtggat ttaatcacgg taacattttg ctggcttaaa gcatctgcca
     2341 gacgctcaac ggcgttaccg acccaggtgt cttgatggag aaactcgctt tcaacaatta
     2401 atactttcat cattgcttac ccggttatga aggaatcttc gttgcgggca agtatcttcc
     2461 ggccttttcg ggctgtaaag cgcgtgaaaa acaaacattt ttcaaatatt tgtttttata
     2521 aacaatgctt tatgtgatta ttttaaatta ttttcactgc ttattatcga cggctaaact
     2581 atttttttgg ctgatactga tatcgtcttt agccgggaaa cgtctggcgg cgctgttggc
     2641 taagtttgcg gtattgttgc ggcgacatgc cgacatattt gccgaacgtg ctgtaaaaac
     2701 gactacttga acgaaagcct gccgtcaggg caatatcgag aatactttta tcggtatcgc
     2761 tcagtaacgc gcgaacgtgg ttgatgcgca tcgcggtaat gtactgtttc atcgtcaatt
     2821 gcatgacccg ctggaatatc cccattgcat agttggcgtt aagtttgacg tgctcagcca
     2881 catcgttgat ggtcagcgcc tgatcatagt tttcggcaat aaagcccagc atctggctaa
     2941 cataaaattg cgcatggcgc gagacgctgt ttttgtgtgt gcgcgaggtt ttattgacca
     3001 gaatcggttc ccagccagag aggctaaatc gcttgagcat caggccaatt tcatcaatgg
     3061 cgagctggcg aatttgttca ttcggactgt ttaattcctg ctgccagcgg cgtacttcaa
     3121 acgggctaag ttgctgtgtg gcaagtgatt tgatcaccat gccgtgagtg acgtggttaa
     3181 tcaggtcttt atccagcggc caggagagaa acagatgcat cggcagatta aaaatcgcca
     3241 tgctctgaca ggttccggta tctgttagtt ggtgcggtgt acaggcccag aacagcgtga
     3301 tatgaccctg attgatattc actttttcat tgttgatcag gtattccaca tcgccatcga
     3361 aaggcacatt cacttcgacc tgaccatgcc agtggctggt gggcatgata tgcggtgcgc
     3421 gaaactcaat ctccatccgc tggtattccg aatacagcga cagcgggctg cgggtctgtt
     3481 tttcgtcgct gctgcacata aacgtatctg tattcatgga tggctctctt tcctggaata
     3541 tcagaattat ggcaggagtg agggaggatg actgcgagtg ggagcacggt tttcaccctc
     3601 ttcccagagg ggcgagggga gtgtccgagc atcatgaggc cgaaaactct gcttttcagg
     3661 tgaattattc ccataaactc agatttactg ctgcttcacg caggatctga gtttatggga
     3721 atgctcaacc tggaagccgg aggttttctg cagattcgcc tgccatgatg aagttattca
     3781 agcaagccag gagatctgca tgatgtctgc acccaaaatt acatttatcg gcgctggttc
     3841 gacgattttc gttaaaaata ttcttggtga tgtgttccat cgcgaggcgc tgaaaacggc
     3901 gcatattgcc ctgatggaca ttgaccccac ccgcctggaa gagtcgcata ttgtggtgcg
     3961 taagctgatg gattcagcag gggccagcgg caaaatcacc tgccacaccc aacagaaaga
     4021 agccttagag gatgccgatt ttgtcgtggt ggcatttcag attggcggtt atgaaccttg
     4081 cacggtgact gatttcgagg tctgtaagcg gcatggtctg gaacaaacca ttgccgatac
     4141 gttggggccg ggcggtatta tgcgcgcgct acgtaccatt ccgcatctgt ggcaaatttg
     4201 cgaggacatg acggaagtct gccccgatgc caccatgctc aactatgtta acccaatggc
     4261 gatgaatacc tgggcgatgt atgcccgcta tccgcatatc aaacaggtcg ggctgtgcca
     4321 ttcggtgcag ggaacggcgg aagagctggc gcgtgacctc aatatcgacc cggctacgct
     4381 gcgttaccgt tgcgcaggta tcaaccatat ggcgttttac ctggagctgg agcgcaaaac
     4441 cgccgacggc agttacgtga atctctaccc ggaactgctg gcggcttatg acgcagggca
     4501 ggcaccgaag ccgaatattc acggcaatac tcgctgccag aatattgtgc gctatgaaat
     4561 gttcaaaaag ctgggctatt tcgtcacgga atcgtcagaa cattttgctg agtacacacc
     4621 gtggtttatt aagccaggtc gtgaggattt gattgagcgt tataaagtac cgctggatga
     4681 gtacccgaaa cgctgcgtcg agcagctggc gaactggcat aaagagctgg aggagtataa
     4741 aaacgcctcc cggattgata ttaaaccgtc acgggaatat gccagcacaa tcatgaacgc
     4801 tatctggacg ggcgagccga gtgtgattta cggcaacgtc cgtaacgatg gtttgattga
     4861 taacctgcca caaggatgtt gcgtggaagt agcctgtctg gttgatgcta atggcattca
     4921 gccgaccaaa gtcggtacgc taccttcgca tctggccgcc ctgatgcaaa ccaacattaa
     4981 cgtacagacg ctgctgaccg aagctattct tacggaaaat cgcgaccgtg tttaccacgc
     5041 cgcgatgatg gacccgcata ctgccgccgt gctgggcatt gacgaaatat atgctcttgt
     5101 tgacgacctg attgccgccc acggcgactg gctgccaggc tggttgcacc gttaaaacgc
     5161 gactaaacgc tactgcgccg ggggatttat tccggcgcac acctctgacg ataccaataa
     5221 cagaaggcgg gcgttggtaa cagcgacccg ataccctatg agcatttcaa tgactacaaa
     5281 actcagttat ggatttggag cgttcgggaa ggattttgcg atcggcattg tgtatatgta
     5341 cctcatgtat tactacaccg atgtcgtcgg gctgtctgtg ggtttggtcg gtactttgtt
     5401 tctggtggcg aggatctggg atgctattaa cgatccgatt atgggatgga ttgtaaatgc
     5461 tacgcgatcg cgatggggta agttcaaacc ctggatcctg atcggtacgt tggcaaactc
     5521 tgtaatctta tttctcctct ttagtgcgca tctgtttgaa ggtactactc agattgcctt
     5581 tgtttgcgtg acctacatcc tctggggcat gacttacacc attatggata ttcccttctg
     5641 gtcgctggtt ccaaccatca cgctcgataa acgtgagcgt gaacaactgg ttccttatcc
     5701 gcgttttttt gccagtctgg cgggctttgt tacggcaggt gtgacgctac catttgttaa
     5761 ttatgtcggc ggtggcgatc ggggatttgg ctttcagatg ttcacactgg tactgatcgc
     5821 cttttttatt gtttcaacca tcatcactct gcgcaatgtg catgaagtct tttcgtcaga
     5881 caatcaaccg tctgctgaag gaagccatct gacacttaaa gccatcgttg cgcttattta
     5941 taaaaacgat cagctttcat gcctcttggg tatggctctt gcttataatg tagccagcaa
     6001 cattattacc ggctttgcta tctattattt ctcatatgtt atcggtgatg cggatttgtt
     6061 cccctattat ctgtcgtatg cgggagctgc taacctggtg acgttagtat tcttcccacg
     6121 cttagttaaa tcattatccc gacgcatttt atgggccgga gcatctattc ttccggtgtt
     6181 aagctgtggt gttctcctgt taatggcatt aatgagctat cacaacgtcg tcctcattgt
     6241 gattgcgggt attttgctga atgtgggaac ggcgcttttc tgggtattac aggtcatcat
     6301 ggtggcagat accgttgatt acggtgaata taaactgcac gtacgctgtg aaagcatcgc
     6361 ttactccgtg cagactatgg tggtgaaggg cggttcagcc tttgcggctt ttttcattgc
     6421 ggttgtgtta gggatgattg gctatgtacc gaatgttgaa cagtctacgc aagccctatt
     6481 aggtatgcag tttattatga ttgctctacc gactctgttt ttcatggtaa cgctgattct
     6541 ctacttccgt ttctatcgcc tcaatggcga cacgctgcgc aggatccaga ttcatctgct
     6601 ggataaatat cgcaaagtac cgcccgagcc tgttcatgct gatattccgg tcggtgcagt
     6661 gagtgatgtg aaagcctgac gtggacaata gctaacgaaa atggcctgat gcgatatgtt
     6721 tatcaggcca cgtcttaatg taatgctttg gatttgcaaa ttttgtagac cggataagga
     6781 attcacgccg catccggcat caacaaagtg cacgttgtta tccggttatc aagccaaagc
     6841 gccgtagctg gcggcaatga aagcgagcga ggaatatcac cgtcgttaat gcgccaagta
     6901 gcgcgcagaa catatcggat tgcgtgtccc actggtcgcc ctgggtgccg agaaaatcat
     6961 ccgctccctg gcccattgcc agcgccgccc accactcgat taattcatac atcgcgctta
     7021 tcgccagcgc cacgcaacag accagaaacg ccaccatttt acgtccgcga acgtacatcc
     7081 cgcgcacaag gatctcacgt gccaccagtg caggcaccag cccctggaaa aaatgcccca
     7141 gcttgtcata cggattacgg ctcaacccca gccattcctg cacctcaaaa ccgatgggga
     7201 ctttcgcgta ggtgtattgc ccgccgacca tcaggatgat ggcgtgaaag aaaatgagcg
     7261 tatagagcag cggcgttaac ggataacgtt tcgcggtggc aagcagtagc ggcacgacaa
     7321 taatcaccgg tgtcacttcc atcagccagg tgagtttgtc atgggccgaa atgccggtat
     7381 aaatcaggat taacgttagc gccagtgcgc cggtgttaag aattaacggc ttgagtgtgc
     7441 gggtcatggt gagttcgcaa gtcagggaaa atactgacta ttcaccagca agcgtgaaat
     7501 tacaatcgca aaagaggtgg cccaggggga tcacctggca gcatgctgcc aggcgctggg
     7561 ccgaagaggt tacttagtgc agttcgcgca ctgtttgttg acgatttgct ggaagaagtc
     7621 gttaccttta tcatcgacca ggataaacgc cgggaaatct tctacttcga ttttccagat
     7681 agcttccata cccagctccg gataagcgac gcattccaga tgcttgatgc tctgctgcgc
     7741 cagtaccgcc gccggaccgc cgatgctacc gaggtaaaag ccgccgtgtt tatggcacgc
     7801 gtcggtaacc tgctggctgc ggttgccttt cgccagcatg atcatgctgc cgccgtggga
     7861 ttgcagcaga tccacgtagg agtccatacg acctgcggtg gttgggccaa gtgaacctga
     7921 tggataaccg gcaggggttt tcgccggacc cgcgtagtag atcgggtgat ctttgatgta
     7981 ctgcggaagt tctttaccgg cgtcaatcag ctctttcagc ttggcgtgtg caatatctcg
     8041 acccacaata atggtgccgg tgagcgacaa acgggtggat accggatatt gcgaaagctg
     8101 ggcgaggatc tctttcatcg ggcggttaag gtcgaccttc accgcttcgc cttcaccggc
     8161 ctgacgcagt tcttgtggaa tgtactggcc tggattgtgt tccagttttt cgatccagat
     8221 accttcgcgg ttgattttcg ctttaatgtt acggtcagcg gagcaggaga cgcccatgcc
     8281 taccgggcag gatgcgccgt gacgtggcag acggataacg cgaatgtcgt gcgcgaagta
     8341 tttaccgcca aactgcgcgc caagaccgag tttctgggcc tcttccagca gttcctgttc
     8401 cagctggaca tcgcggaacg cctgaccatg ttcgttccct tccgtcggca gttcatcgta
     8461 atagtgagcg cttgctaact tgacggtttt caggttggtt tccgcagacg taccgccaat
     8521 cacaaacgcg atatggtacg gcgggcaggc tgcagtaccg agggtacgca ttttctcgac
     8581 gaggaagttt ttcagtttgc cgggagtcag cagggctttg gtttcctggt agagatacgt
     8641 tttgttggca gagccgccgc ctttcgcaac gcaaaggaat ttgtactcat cgccatctac
     8701 cgcgtacagg tcgatttgtg caggcaggtt agtgccggtg ttgacctctt tgtacatgtc
     8761 cagcgccgcg ttttgtgaat agcgcaggtt atcttcgata taggtgttat agacgccttt
     8821 cgacagcgct tcttcatcac cgccgccggt ccacacgcgc tggccttttt taccgacgat
     8881 gatcgcggtg ccggtatcct ggcaggtagg cagcacgcct ttggcggcga tttcggagtt
     8941 tcttaagaat tgcagcgcca cgtacttgtc gttttcgctg gcttctggat cgtgaagaat
     9001 agccgcaacc tgtttctggt gtgccggacg gagcataaaa gaagcgtcgt gaaaggcttg
     9061 ctgcgccagc agggtcaggg cttctggttc cactttcagg atggtttcgc cgtcgaagtc
     9121 ggcaacgcta acgtaatcgg aagtgagtag atagtattcg gtattgtctt tccccatcgg
     9181 gaaaggtgcc tggtagataa agggtttgtt tgacatagct tccagcctgt aactctgtat
     9241 ttgttattcg aaaatggcgt gccgcgtagt cacgggcggc acgcaaagtg catttataag
     9301 aacccgtaca tcgcggcgaa gatccagccg aagacgcacg atacgctcac accaatcaac
     9361 cccggcagaa taaagctgtg gttgatgacg aagcgaccga tgtgggtggt gccggaacgg
     9421 tcaaactgaa tcgctgccag atcgctcgga taagtcggca ggatgtaata accgtagcaa
     9481 gccggtgctg aagccacgat gtatgccgga tcaacgccga tcgccagcgc gaccggaaca
     9541 atcgccgcca gcgccgcagc ctgagagttt acaaacttgg aaaccagcag cagaacaatg
     9601 gcataggccc acggatactc tttcaccatt tcacccagta cgccctgaat ttcagacata
     9661 tgcgcaccga acatggtttc tgccatccat gcgataccgt acaccgccac gatggcgatc
     9721 ataccggaac ggaagacttc gttttttgag atagacgcgg gattggtttt ggtcaggata
     9781 ataatcagcg ccccggtcag cagcataaac atctgaataa ccagtaccat cgacagcggt
     9841 ttgccgccga aggatggacg caggtccgaa tcagcaccaa gaagtgcgac tacagcgatt
     9901 gccccgagga aaatccacat tgccagccag ttgcttttcg gcagtttttt atccagcagc
     9961 gtcgcggtat caccgtaaac atactcacgg ttttccggta cggagatgaa tttctggaac
    10021 tcttcgtctt tatccagatc tttaccgcgg aaccagctga agataccgat cgccaggata
    10081 ccgattaacg tcgatggaat ggtgattgcc agcaggtcga ggaactcaag atggcgacca
    10141 tcaaaggtga cattacccag cattgcaacc agagacacga ccgcaaccga caccggactg
    10201 gcgataatcc ccatctgtgc accgatagaa cttgccgcca tcggacgttc cggacggatg
    10261 ttgttcttaa tggcgacgtc gtagatgatc ggcagaatgg tgtaaaccac atgacccgta
    10321 ccgcaaagaa tggtcagcgt acaggtcaca aacggcgcga caattgagac atatttcggg
    10381 ttgcggcgca gcagcttctc ggcaatttgc agcatgacat caagaccgcc cgaagcttgc
    10441 aaggtcgccg atgccgccac caccgcaatg ataaccagca tgacatcaac tggtggttta
    10501 cctggctgaa ggtggaagac gaagaccaga atgaccagac cgataccgcc taataaaccc
    10561 agcgcgatac caccctttct ggcaccataa aacagacata tcagtattat gataagttgg
    10621 atagtaaata acatgtgtga accctcgcga taatcctatt taaatttttg ctgaatagat
    10681 cacagtcacg ttctgttttg tatgaactgt tttcagagcc tgactaaata tccgtttggt
    10741 cgttaccggc tttagcaaat acctcacagt gaatattggc tgattaaagc agcggcaaat
    10801 ttatgcactg tgataagcgg ctttttcaga gagaaaaact ctctggcgat gggtttaaag
    10861 tttgtactgc gcagatgtta atgttttgtt tgctgttatc ttattgatat ttatcggtct
    10921 attttttgtt ctcagggcag atatcattag gtttatagat taatctgatc tacccatttg
    10981 tgggtaaaaa tacacataat gcgggtgaca taatagttaa ttaacttttg ttagcgtttt
    11041 gaaattaaaa acaccgttca cctgaagaga tattaatttt tagcgatgat ggagggataa
    11101 ttatatttga tctggcacaa gttttactga tgaaggatgt aacttgtgcc aggggtattt
    11161 tgcattacgg taataattat tacttacaga taaccagcga tccgttattg gcaatattgt
    11221 ttcagtagtg agtagtgttc tgcctgaata cggtaacggt aaaccggacg ccccgtgacg
    11281 ccataatgga tactggtgaa caagatgtgg cagttgacca gccagatgag gtatttacgg
    11341 caggaaacac gcgagatgtt aacctcgtta gccagctcgt cggttgaaaa ctcatagtcc
    11401 tgatgcgcgt caatccattg gcacagcgtg cgtaacgtct gcggcgttaa gccttttggc
    11461 aagcgacgag gatcctgttc gttggagctg ctgccgtgga ttagctgatc aagctcggcc
    11521 tggtcataat actgatgttt ttccagcgcc attttctttt gccgccagcc ggtgagcgcc
    11581 tcttcaaagc gggaagcctg gaagggtttg atcaggtaat ccacgacacc gtaatgcagc
    11641 gaatctttaa tggttgccgc atcggctgcg gaggagatga caatcacgtc acttttgcaa
    11701 cgcgcgttat gcaggacagg cagtaaatcg agcccgttct ctttttgcat atagatatcg
    11761 agcaatatca ggtcgatagg cgtatcgcta ttgaagataa tctctttggc tttctccagc
    11821 gtcgaggctg ttccacagca ttgaaagcct gggatttgtg ctacgtatcg gcgattcagc
    11881 tccgcgacca ttgcgtcgtc atcgataatt aatacattga tcatctgttc gacctctccc
    11941 cgtcccaggg tatctggaca aaaaattgtg tgaaaatccc gggttccgat tccacggcga
    12001 tgctgccgcc gagattttct acctgttgtt tgacaagtgc taaaccgacg cctcgctcgc
    12061 ttccttttgt cgagacacct ttgtcaaaaa tgtgatcgat tttgtcgggt gcgatccccg
    12121 gtccatcatc gttaacttcg cagtgcagcc agccgtgacg gtagtgcaat gttacgctaa
    12181 tttcgccacc gggttccggc cctaatgctt ccagcgcgtt ttctatcaga tttcccaatg
    12241 tggtaatcag cgtcgcgacc tggtcctcac tgccgctgtc tggcagctgg ctttcactgt
    12301 ttaaaatcag cgtatggcct aaatcggtcg cgcggttaat cttgctgatt aaaaaaccag
    12361 cgataaccgg agatttgatc ttacccagca gagagccaat ctcttcctga tagttattgg
    12421 ctgttttgag aatgtaatct tccaactgct tataactctt cagatgcaat aatccgagaa
    12481 tcacatgcaa tttattcata aattcgtggg atcgttcacg aagtgcgtca gcatagttga
    12541 ccagaccatc gagtcgctgc atcagtttac gtacttcggt tttgtccctg aaggttgaaa
    12601 tggcaccgat gataacgcca ttactgcgca ccggaacggt gttgatcagt aatagccgat
    12661 ctttaatcgt aatctcttcg tcgcggcgcg gggtaccgtc gcgtaacact tccgagacat
    12721 ctaccacctg tgaccacgag tggcttagcg tcgacagttt ctcatcgtcc tgcgacttac
    12781 ggtaattcag caattcttgt gcggcatcgt tgatcagcgt gacctcgccg cgatcgtcca
    12841 cggcaacgac gccttctttg atagactgca acatggcctg gcgttgctca aacagcgtgg
    12901 agatttcgta gggttccagg ccgaaaagga tttttttcag tactttaacc agaatgcagg
    12961 tgccaatcag tccgaccagc atgccaaata atactgacca gataatgctc cagcgactgt
    13021 cattgatctg ttgggtcacg cggcttaact caaggccgat cgccaccacg ccaatttgtt
    13081 tatgattttc atcgtagatg ggggtaaata cgcgtaaagc ctgcgccaga aaaccgcgat
    13141 tgatagcgac attttcttcg ccattcagcg ctttaaggat gtcatcacct ttaaatggct
    13201 gaccaatacg ctgggcttca ggatgcgagt agcgaagact ttgcatatcg gtaacgacaa
    13261 taaacagcag atcgttgcgt ttgcgcacgg cttccgcgat ggcctggatg ccgctctcct
    13321 gcggtttttt ctgcaagccc tgacggattt ccggcgagtc ggcgagagta cgcgccactg
    13381 ccagtgcctt gttggctagc ccgtctcgcg tcatatcact gatttgcgag aagtaaatca
    13441 gatgcaccac caatagcacc gagaacagta ccgcgctgac cattaagatc actgtggtac
    13501 tcaatttcat cggacgtttg cgtaacatgc ggtagggcaa tgaatgtctc atcagcttcc
    13561 ttgtgtgaca aatttcttaa gcattatctc tgatgaggcg ggtaattcaa agggagtaag
    13621 aatgattggc tatatagggg aagagactct ggcaacggaa actgccagtg ctgtatgaag
    13681 attccagggc tatgcttata gtgaaaacca tactgatgag agagggaagg tcatggatca
    13741 ggcgctactg gacgggggtt atcgctgtta taccggcgaa aagatcgatg tctatttcaa
    13801 cactgcgata tgtcagcatt ctggcaattg cgtacgtggc aacggcaagt tatttaatct
    13861 caaacgaaag ccgtggatca tgccggatga agtcgacgtt gccactgtgg tgaaagtgat
    13921 tgatacgtgc ccgagtggtg cgctgaaata ccgtcataaa taagcgaggg taaaatggaa
    13981 atacgcgaag gccacaataa attttacatt aatgacgaac aaggcaagca aatcgctgaa
    14041 attgtctttg tgccgaccgg agagaattta gcaattatag aacataccga tgtcgatgaa
    14101 agcctgaaag ggcaagggat cggtaaacag ctggttgcga aagtagtgga aaaaatgcgt
    14161 cgggaaaaac gaaaaattat cccactatgc ccatttgcga aacacgaatt tgataaaacg
    14221 cgggagtatg atgatattcg cagttgatgg aagagtacag agccacgata tttttcatgc
    14281 tctccgcgga gtgatgcagg agagcatctg aagggtaggg ggatgcacaa agaatgggca
    14341 gagagcgcgt ttttttgtcc caagtcatcc cctttactga gcaaaaaaaa gaatatctcc
    14401 tatatgagaa tcatcaatcg gggttaataa gttttgcgtc cccagagcgt tcaatattga
    14461 taggagtcat attatggaag gtaaaaacaa gttcaatact tatgttgttt cttttgatta
    14521 tccatcatct tattcctcag tgttcttaag gttaagatca ttgatgtatg atatgaattt
    14581 ctcctctatc gtggctgatg aatatgggat accacgacaa ttgaatgaaa actccttcgc
    14641 aataacgaca tcgttagccg caagtgaaat cgaagattta atcaggctta aatgcttaga
    14701 cttaccggat attgactttg acctcaacat tatgacagtt gatgactatt tccgtcagtt
    14761 ttaca
//
LOCUS       DAZUCE010000119        14758 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZUCE010000119 DAZUCE010000000
VERSION     DAZUCE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.119"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            151..693
                     /gene="yfaZ"
                     /locus_tag="ACHY03_003084"
     CDS             151..693
                     /gene="yfaZ"
                     /locus_tag="ACHY03_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFS3906285.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            793..1995
                     /gene="yfaY"
                     /locus_tag="ACHY03_003085"
     CDS             793..1995
                     /gene="yfaY"
                     /locus_tag="ACHY03_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFS3906286.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2215..2997
                     /gene="yfaX"
                     /locus_tag="ACHY03_003086"
     CDS             2215..2997
                     /gene="yfaX"
                     /locus_tag="ACHY03_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFS3906287.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            3012..4217
                     /gene="rhmD"
                     /locus_tag="ACHY03_003087"
     CDS             3012..4217
                     /gene="rhmD"
                     /locus_tag="ACHY03_003087"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFS3906288.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4274..5563
                     /gene="yfaV"
                     /locus_tag="ACHY03_003088"
     CDS             4274..5563
                     /gene="yfaV"
                     /locus_tag="ACHY03_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3906289.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5581..6384
                     /gene="yfaU"
                     /locus_tag="ACHY03_003089"
     CDS             5581..6384
                     /gene="yfaU"
                     /locus_tag="ACHY03_003089"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFS3906290.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVDGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6425..6634)
                     /locus_tag="ACHY03_003090"
                     /pseudo
     CDS             complement(6425..6634)
                     /locus_tag="ACHY03_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6635..7630)
                     /gene="rpnE"
                     /locus_tag="ACHY03_003091"
     CDS             complement(6635..7630)
                     /gene="rpnE"
                     /locus_tag="ACHY03_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFS3906291.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPKLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     QGKLEGLQKGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCH"
     gene            complement(7823..9013)
                     /gene="glpC"
                     /locus_tag="ACHY03_003092"
     CDS             complement(7823..9013)
                     /gene="glpC"
                     /locus_tag="ACHY03_003092"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFS3906292.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9010..10269)
                     /gene="glpB"
                     /locus_tag="ACHY03_003093"
     CDS             complement(9010..10269)
                     /gene="glpB"
                     /locus_tag="ACHY03_003093"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFS3906293.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLHSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10259..11887)
                     /gene="glpA"
                     /locus_tag="ACHY03_003094"
     CDS             complement(10259..11887)
                     /gene="glpA"
                     /locus_tag="ACHY03_003094"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFS3906294.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDIS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLILDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLYGLEKEQKDAL"
     gene            12160..13518
                     /gene="glpT"
                     /locus_tag="ACHY03_003095"
     CDS             12160..13518
                     /gene="glpT"
                     /locus_tag="ACHY03_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFS3906295.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            13523..14599
                     /gene="glpQ"
                     /locus_tag="ACHY03_003096"
     CDS             13523..14599
                     /gene="glpQ"
                     /locus_tag="ACHY03_003096"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS3906296.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEALKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT         3372 a         3659 c         4019 g         3708 t
ORIGIN      
        1 tgtttatttt ttcggcgttt aattatttaa gaagtattta gttacaataa gtgactagat
       61 cccttttttc gctcattgaa tcctctgcgc caggcagtac aatcccgcag ctttgggaat
      121 tttgattaat tgcttgtggg aaaggggata atgaaaaaaa ttgcgctagc aggtctggcc
      181 ggaatgctgt tggtttctgc atcggtcaat gcaatgagca tcagcggcca ggcgggtaaa
      241 gaatacacca atattggtgt cggttttggg actgaatcga cgggcctggc tttaagcggt
      301 aactggacac ataacgacga cgacggtgac gtcgcgggcg tggggctggg gttgaatctg
      361 cctctcgggc cgttaatggc gaccgttggc ggaaaaggcg tttacaccaa cccgaattac
      421 ggcgatgaag gttatgccgc agcggtagga ggtggtttgc agtggaaaat tggcaacagc
      481 ttccgtttgt ttggcgagta ttactactct ccggattcac tctccagcgg tattaaaagt
      541 tatgaggaag cgaatgctgg cgcgcgttac accattatgc gtccagtcag tattgaggcg
      601 ggttatcgct acctgaatct gtcgggtaaa gacggtaacc gcgacaacgc cgtggctgac
      661 ggcccgtatg ttggggttaa cgccagtttc tgatcccttc cggcgcggca ttctgtcgcg
      721 ccattcagtt tcttactacg ccttatctcc ctttgcgtta tagtgattcg ataacaaaag
      781 caggagataa ccatgttaaa agtggaaatg ctatccaccg gggatgaagt gttacacggg
      841 caaatcgttg acactaacgc tgcctggctg gccgattttt tctttcatca ggggttgcca
      901 ttatctcgcc gcaatacggt gggggataac cttgatgact tagtcaccat tcttcgcgaa
      961 cgtagtcagc acgccgatgt gctgatcgtt aacggcgggc tgggaccgac cagcgatgat
     1021 ttaagcgcac tcgccgctgc gacagcaaaa ggtgaaggcc tggtgctgca tgaagcctgg
     1081 ctcaaagaga tggaacgcta tttccacgaa cgtggacgag taatggcacc gagcaaccgt
     1141 aaacaagcgg agctgcctgc cagtgctgaa tttatcaata acccagtagg caccgcctgt
     1201 ggttttgccg tgcagcttaa tcgttgcctg atgttcttta ctcccggcgt accgtcagaa
     1261 tttaaggtga tggtcgagca cgaaatcctg ccgcgtctgc gcgagcgttt ttctttacca
     1321 cagccgccgg tttgcctgcg tttgactacc tttggtcgtt cggaaagcga tctggcacaa
     1381 agcctggaca ctctacaact gccgccgggc gtaacaatgg gctatcgctc ctcaatgccg
     1441 atcatcgaac tgaaactcac cggaccggca agcgagcaac aggcgatgga aaaactgtgg
     1501 ctggacgtta aacgtgttgc cggacagagc gtgattttcg aaggcaccga aggactgccc
     1561 gcgcagatca gtcgcgaatt gcaaaaccgc cagttcagcc tgacgttgag cgagcaattc
     1621 accggtggtt tattggcttt gcaactttct cgcgcaggtg ctccactgct ggcgtgtgaa
     1681 gtggttcctt cacaggaaga aaccctggcg caaactgcgc actggattac agaacggcgg
     1741 gccaaccatt ttgccgggct ggcactggct gtttcgggtt tcgagaacga gcatctcaac
     1801 tttgcgctag ccacgccaga tggcactttc gctctgcgtg tgcgtttcag cactacacgc
     1861 tacagcctgg ctatccgtca ggaagtgtgc gcaatgatgg cactgaatat gctacgccgt
     1921 tggttaaacg gccaggacat tgccagtgag catggctgga ttgaggttgt tgagtccatg
     1981 accttatctg tctgattccg ccactctgtg ccggtttact gcaccggcac attccccctc
     2041 cggcaaaatt tgtccaacct gttgtactca ataagaaaaa atccgtgcgc tgattcacaa
     2101 atttgtgcgt aatttctcca gtccgttgga cgaggagcgg gatttgatgc gcttcacaat
     2161 tttactcccc ctgagcaacg agactgattc cagtatctaa gactggagtc cagtatgttg
     2221 gaatcaagca aagttcccgc cctgacacgg gctattgata ttctcaattt gattgcccgc
     2281 attggcccct gtaatgctgc gacgatcatt gacacattgg ggatccctaa aagcacggcc
     2341 tatttgctgc ttaatgaact caggcgtcag cgttttctta gccttgatca ccaggaaaat
     2401 ttttgtttgt ggaccaagct ggtggagctt tccggtcatg cgttaagcaa gatggatctc
     2461 cgggaactgg ctcgaccgcg tttgacgcaa ctaatggata ccaccgggct gttatgtcac
     2521 ctggggatta ttgataacgg cagcgcttat tacattttga aagtggagtc atcagccact
     2581 atcagcgtgc gttcacatga aggaaaaagc ctttcgcttt atcgctccgg aataggtaaa
     2641 tgcctgcttg cctggcaacc tgcaactgta cagcaaagta ttatcgaagg gctggtctgg
     2701 gagcaggcga cacccacaac aattactcat ccacagcaac ttcacgaaga actggcgcgt
     2761 attcgccgtc agggctggag ctatgacaac ggcgaagatt acgccgatgt ccgttgtgtc
     2821 gctgcgccgg tgtttaacgc caataacgag cttaccgctg cgatctcggt ggtcggtacc
     2881 cgcttacaaa tcaatgaaga ataccgtgat tatctggcgg gcaaagccat tgcctgcgcc
     2941 agggatattt cacgtctgtt gggatggaaa agtccctttg acttacaagc ctcataacaa
     3001 tggagaacat catgacccta ccaaaaatta aacaggttcg cgcctggttt actggcggtg
     3061 cgacagcaga aaaaggcgct ggcggcggtg attatcacga ccagggggcg aatcactgga
     3121 tagacgatca tattgccacc ccgatgagta aataccgcga ttacgagcag tcacgccagt
     3181 catttggcat taacgttctt ggcacgttgg ttgttgaagt cgaagcagaa aacggccaga
     3241 ccggattcgc tgtttcgaca gccggtgaaa tgggctgttt tatcgtcgaa aaacacctta
     3301 accgtttcat tgagggtaaa tgtgtcagtg atatcaaact gatccacgat caaatgctca
     3361 atgccaccct gtattactcc ggctctggtg gcctggtgat gaatacgatt tcctgtgtcg
     3421 atctggctct gtgggatctg ttcggcaaag tggtcgggct tccggtttat aaacttttag
     3481 gcggcgctgt tcgtgatgag attcagttct acgccacagg tgcgcgtccg gatctggcaa
     3541 aagagatggg ctttatcggt ggcaaaatgc cgacgcactg ggggccacat gatggcgatg
     3601 cggggatccg caaagatgcc gctatggtcg cggatatgcg tgaaaaatgc ggtgaggatt
     3661 tctggttaat gctcgactgc tggatgagtc aggacgtgaa ctatgccacc aaactggccc
     3721 acgcttgcgc gccctataac ctgaaatgga tcgaagagtg cctgccgcca cagcagtatg
     3781 aaggttatcg cgaactgaaa cgcaacgcgc cagtcggaat gatggtcacc agcggtgagc
     3841 accacggcac actgcaatct tttcgtacgc tttcagaaac cggtatcgac attatgcagc
     3901 cggatgttgg ctggtgcggt ggcttaacca cgctggtgga aattgccgca atcgccaaat
     3961 ccagggggca actggtggtg ccgcacggtt cgtctgttta ctcccaccat gcggtgatca
     4021 ccttcaccaa tacgccattc agcgaattcc tgatgaccag tccggattgt tcaacgatgc
     4081 gtccgcagtt tgacccgatt ctgctcaatg agccggttcc ggtgaatggt cgtattcata
     4141 aatcagtgct tgataaaccc ggttttggcg tcgaactcaa tcgtgactgc aatttgaaac
     4201 gcccctacag ccactaatca ctccgggcgt tgctgtgaca acggtaacgc ccacatattc
     4261 taaggagctt tttatgagca ccgctttgct tgacgccgtg gtgaagaaaa accgtgtgcg
     4321 tttaattccg tttatgttgg cgctgtatgt gctggcgttt ctcgaccgtt cgaatatcgg
     4381 ttttgccaaa cagacctacc agattgatac cgggttgagt aatgaagctt atgcgctggg
     4441 agcaggcatt ttctttgtgg tatatgcgtt tctgggtgtt ccggcgaatc ttttgatgcg
     4501 caaactgggg gccagaacct ggattggtac gacaacactg ctgtggggat ttctttcggc
     4561 agccatggca tgggccgata ctgaagcgaa atttctgata gttcgcactc tgcttggtgc
     4621 tgcggaggcc ggatttttcc ctggtatgat ttatctcacc tcgcaatggt ttccgcagcg
     4681 taatcgcgcc agcattatgg ggctgttcta tatgggcgca ccgctggcgt taacactggg
     4741 atcaccgctt tccggcgcgc tgctggagat gcatggattt atggggcatc ccggctggtt
     4801 ctggatgttt gtgattgaag gattgttggc agtcggcgct ggggtattca cattcttttg
     4861 gcttgatgac acaccggagc aggcacgttt tctgagtaaa caagaaaaaa cgttgcttat
     4921 caatcaactg gcaagtgaag aacaacagaa agtgacttct cggctgagcg atgcgctgcg
     4981 taatgggcga gtctggcaac tggcgattat ctacctgacc attcaggtgg cggtttacgg
     5041 attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag tgggctttac
     5101 agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct ggcttattcc
     5161 gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat tactggcggc
     5221 gggcattggt attggtctgt ccgggctgct ttctccagta ctggcgatcg tagcgctgtg
     5281 tgttgcagcc atcgggttta ttgccgtgca gccggtgttc tggacgatgc cgacacaact
     5341 gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgtttg gtgcagtggg
     5401 cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttattttcca gcgatgcggc
     5461 gggattactg acgctggcag cggtggcggt catcggttcg ctgattattt tcactctgcg
     5521 tgtaaatcgt actgttgcgc agaccgacgt ggcacatcat taaataggtt aaggaacacg
     5581 atgaacgcat tattaaccaa tccctttaaa gaacgtttac gcaagggcga agtgcaaatt
     5641 ggtctgtggt taagctcaac aactgcctat atggcagaaa ttgccgccac ttccggttat
     5701 gactggttgc tgattgatgg ggagcacgcg ccaaacacca ttcaggatct ttatcaccag
     5761 ctacaggcgg tagcgcccta tgccagccaa cccgtgatcc gtccggtgga tggcagtaag
     5821 tcgctgatta aacaagtcct ggatattggc gcgcaaactc tactgatccc gatggtcgat
     5881 actgccgatc aggcacgtca ggtggtgtct gccacgcgct atcctcccta cggtgaacgt
     5941 ggtgttgggg ccagtgtggc acgggctgcg cgctggggac gcattgagaa ttacatggcg
     6001 caagtcaacg attcgctttg tctgttagtg caggtggaaa gtaaaacggc actggataac
     6061 ctggacgaga tcctcgacgt cgaagggatt gatggcgtgt ttattggacc tgcggatctt
     6121 tctgcgtcgt tgggctaccc ggataacgcc gggcacccgg aagtgcagcg aattattgaa
     6181 accagtattc ggcggatccg tgctgcgggt aaagcggctg gctttctggc tgtggctcct
     6241 gatatggcgc agcaatgcct ggcgtgggga gcgaactttg tcgctgttgg cgttgacacg
     6301 atgctctaca gcgatgctct ggatcaacga ctggcgatgt ttaaatcagg caaaaatggg
     6361 ccacgcataa aaggtagtta ttgatatcaa aggcccatgg ggatcggctg tgggcctgtg
     6421 ttaattagtg gttattcgct gccagatcgg cttcgcttag ctgggtggcc gcgagcacca
     6481 ggtcacgatc aaagccctgt tccagcatgc gcaaagcaat tttaatggct tgttcatgca
     6541 taccttcctg caaaccttct aatttacctt cctgccaacc aatttggtgt ccttcctgcc
     6601 gtagccgttc agcaatagtc atcaatctct ccttttaatg acagttcgga atatcagcat
     6661 cagtgagttg ggttgtcgcc agcacaatct cacgttcaaa gccctgctcc agcatgcgca
     6721 aggcaatttt aatggcttgc tcatgcatgc cttcctgcaa accttctaat ttacctttct
     6781 gcagaccctc caatttacct tgctgcaaac cttctaattt accttcttgc cagccttcaa
     6841 ttttaccttc ctgccagcca atttggtgtc cttcctgccg tagccgttca gcaatagtca
     6901 ttaatctctc cttttgtagt ggtgaacgtt cggcaatctc ctgaataaaa cgggtgaaac
     6961 gggaggtatc gccgcattgc agcagataat taaacagtgt ttgtagctgg ctgtcattag
     7021 tgaagcctct aaccaaaagc gtggtgatcc tgtcgaccat accgattaaa tcgcggtcgc
     7081 gaatatgctt ttgaatcagt tccagcagag ctatacgccg atgttgcatg atctcatcgt
     7141 caggtacgat ggtaatatcc accaacggaa aagcttcggt gtacaactgc cgggcaagtt
     7201 tcggatcgtc aaactcatcc agccagttga gtgagtacgg gtagggcgag gtttcgccat
     7261 gataaaacag caacggcacc accagcggga ctctgtcata gcctttgtcc aggtgacgct
     7321 gcatggcggc agtggcatag cgcattagcc gaaaagccat attcttttct gcagagcttt
     7381 gatgttcaat cacgcagtag atatagccgt caccgtcgct ggtttccacg gaccacaaaa
     7441 catccgagta gtaagcgcgt aaactttttt caataaaact agtgggttcc aggcgtaagg
     7501 tttgcaggtt gcaaagcttg cgcagtggtt ctggtaaatg tatttcgaga aaatcccgtg
     7561 cggtttcggg tgtgaacata aaggttttaa atactgcatc atgcggggag gaggttgttg
     7621 attcggtcat ggttgtccgt cacctgatat ttatgatgac gcgacattac ggttatccca
     7681 ggcctttgac atctgccagt ttcagccttt gcgagacgtt ttcagaggaa ttttcactgc
     7741 gttgcaacgc atacattggg cacggaatcg tgtacgcaaa aatgcccggt aaccacatgc
     7801 ttaccgggca tcagaaagga gtttaagcca gcgcctgggc cagtagcgta atcggatgtt
     7861 cgcagcgaag acttgtggac atctcaatct gccatttaca ggtttcgcag tcggtgacca
     7921 ccagatctgc gccgctttct tctatctggc ggaacagtgg tgcgccgatg gcttgtgagg
     7981 tggggtagtt ctcttttttg aaaccgtaag tacccgcaat accgcagcac tgggaatcca
     8041 gcaccgttaa ttcaagcccc gggatgttac gcaacagctc cagggtgtag agcgtccagc
     8101 ccattttttc catatggcac ggagtgtgat aaaccacttt cagcggcagc ggtttcagcg
     8161 gtaacgtttt gccttcgtcc agcttgcgcc acagccagcg ggtcgccagt tcgatatgat
     8221 cgcgcaagcc tttgttgtcg acattcagca cttccgggta ttcgtcgcgc agggcaaatg
     8281 tacaggttga ggaggtggca atcaccggaa tgccttttac tcccacagct tcgcggatcg
     8341 actctacatt cgtaattgcc tgtttgcgtg ctttatcggt aaagccgttg gcgatcagcg
     8401 gtacgccgca gcatttttct ttgctgagca gttgtacacc ggtacccatt gcgttgagca
     8461 ctttaattaa atctttgcct aactgcggat ggttgtagtt aacgaagcag ccgtgaaaga
     8521 aagcgacctg gtctttatac tgcgcttgct gagccgccac gctgcgatac cagcgacgaa
     8581 acgtgccgaa ggagtatttc ggtagcgtgc ggcgatgatc gatttttaac gccgcatcaa
     8641 gcagctgccg caccggtttc agcgaggtag cggtgttgac gattggtgcg aacggcgtgg
     8701 aaacgctacc catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgcgtgg
     8761 tgtcatattt cgcccgcgcg cgctggatga tatcgccaat cttcacatcg gacgggcagg
     8821 cgacttcaca acgtttgcag ttgatacaat atttcagcgc ctcgtcatac agtgcgccat
     8881 cttttaaacg cagacgctcg ccatccggcc cggcttgttt tggtcctgga taaccgggat
     8941 tcacccggct cactgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc
     9001 tggtgtcatt cattgttggc ctcctgcgcg ttgggcaatc tgttgagcgg catgtaaagc
     9061 actgacggca caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc cgcccagcac
     9121 cgaaccgatg gcaaacaggt tctcaatggt ttgccctgcc tgtgatgggc gtagcgtctg
     9181 atcagtggtt acaccgaact gctgccacgg ttgcggcgca aaaaaatctc ccttatacca
     9241 ttcaccccgc gtggcggttt gtagcacatc aaggccgaga atcggctctc gaatgccgtt
     9301 acgttccgct accagtccgc cactaaagaa actgccgctg gcgagaaccg cgaaacgtgg
     9361 acgcagcggg atatcggcgt gattgcgggt ccagatttca ttcactacgc catttttaca
     9421 ggtcactttt ttcacttcat cgcccggcat ccacacgcca ccctgacgca caaactggcg
     9481 ctgtaactgg ttttgcagac gaatgcccag cacggaaggt ggcagcgttg gcaaaagcat
     9541 cagtgaacaa ggtagttttt cattcaacca acgccacagt ttgtcatcag ccaggccgaa
     9601 gcaggcgggc atcaggatca tttcgcaggt attggcgaca ggaataagcg catcaagtaa
     9661 cagcggccag ttttcttcat tatcaaggaa acgggcgata ttcaccgcgc ggaactcggt
     9721 ggcgttattg cgcaggacat ccagttccgg cagctctatt tctgcggttt caacggcaag
     9781 gccgagttca cgcaacgaag ctgccgcaag gtgcgcctga aaatccatca gtccgctaat
     9841 tcccactaca catattttct tcgcgggcag cggccagacg gggacttctg gcgaacttag
     9901 ccaggtagag tgcagagtgc cgagcggcgt aatccgctgg tgagccagtt ctacgctgcc
     9961 ctgcaattgc gcaccgcttt cagcgattaa tgcctgcgcc tggcaagcga gatcgagcac
    10021 gcgttgtggc ccgagaagag agtaaggatg tgcaggtgcc tgctgacgca aagattccag
    10081 tccactgtga atgtctgtca cagcttgacc atctggcaga tggctcagca aatccagcga
    10141 accggatgag aaatgcagcg cgctttgacc acgcgtgaca atggcacagc gcaggccgtg
    10201 tttttgcagt tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgacagtatc
    10261 aaagcgcatc tttctgctcc ttctccagac catacaatcc ctgataaacc cagcgggtaa
    10321 attcgctttc gcgcagtgca tctccccagg cgatgggttg cacgcctttc cagcgttcgt
    10381 taaggaaggt ggaaagttgc tcgatagatt gcgcggacgt cgtgacgtta aaacgttgca
    10441 gcagtccggc agcgcggcag gcgcagagtt cgccctggca ggtacccatc cccacacggg
    10501 tacgacggcg taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgcactt
    10561 cacccgcagt taccgcttcg cactcacata ccaggctacg gtgcagacgg ccttcgctca
    10621 gccaggcagg cgtacgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggca
    10681 gggagatgac tttacgcaag gtaacttcag cgggttcttg tgaaccaggc agtgccagat
    10741 cggcagtcgt acaggggcgc gtgttgccca gtttgcggca taccgcgtcg gtagcccatt
    10801 cagccatcag ccgataggtc atcagtttgc caccggtgat ggtgataaat ccgtccagac
    10861 catcgcgttc agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcgggt
    10921 cgtcatcgct ggcaaccagc gggcgcacgc cagaataggc ccgcaaaatg cgcgttttcg
    10981 ccatcacggg ggccagtttt tccccttcac gcagcagaat atcaacctct tctgccgtca
    11041 ctcgattatc gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca atcagcgaaa
    11101 tggtatcgcc aggcaccaga atatcggcgt cggaaggttt acggcagcgg ttgatcacat
    11161 gctggttaat gcggtgatcc aagatcagca gcgatccttt cgccgggaac atgcgaatgc
    11221 gcagatcggc atattcggca atgtgttgcc cccagatccc agcggcatta accacgacag
    11281 gtgcatgaag ggcctgagtt tcgccggtga gatggttacg tacacgaaca ccgcacaccg
    11341 tcgcgccttc acgaatcagc cccgtgactt catgagcggt aaggataaca gcaccgtgtt
    11401 ctttggcatc cagcatgttt gctgcggtca gacgaaatgg atcaacggtg ccatccggaa
    11461 ctttcaccgc gccaatcagt gccgggttaa cggcaggttc gataatgcgc gcttgctgcg
    11521 ggtctatagc ttctgcgctg atccctgctt cttcgcaggc gcgaataaaa gtggcctgga
    11581 aggagatgtc atcttccggc agggtgataa acaggccgtt agttggttca acgcagtgac
    11641 gtgcaatgcg tttcaggatc tggttttcac taatgcattc gcgggccgat tccgcatcgg
    11701 ttaccgcata gcgcgcaccg ctgtgcagca ggccgtggtt acgcccggtg gcaccggttg
    11761 cgatgtcgtg gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag tcgcgggcaa
    11821 tcccggctcc cgttgcgccg ccgccaatga taatcacgtc acttgattgc gagtcgcgag
    11881 ttttcattgt tatccctctg aagttcgttt tttaccattt agccatagta aaaacatgaa
    11941 ttgtttgatt tcgcgcatat tcgctcataa ttcgaaagtg aaacgtgatt tcatgcgtca
    12001 ttctgaacat tttcaccatc gtatttaata atgtgtgcgg taattcacat ttaatttatg
    12061 aatgttttct taacatcgcg gcaactcaag aaacggcagg ttctcccact gaatcaggct
    12121 gttaatcata aataagatca cgggccacgg aggctatcaa tgttgagtat ttttaaacca
    12181 gcgccacaca aagcgcgctt acctgccgcg gagatcgatc cgacttatcg tcgattgcgc
    12241 tggcaaattt tcctggggat attctttggt tatgctgctt actatttggt tcgtaagaac
    12301 tttgcgctcg ctatgcctta tctggttgag cagggattct cacgcggtga tttaggtttt
    12361 gccctttcgg ggatctcgat tgcttatgga ttttcgaaat tcatcatggg ttcggtatcg
    12421 gatcgctcga atccgcgcgt tttcctgccc gcaggtttga ttctggcggc ggcagtgatg
    12481 ttgtttatgg gctttgtgcc atgggcgacg tcgagcattg cggtaatgtt tgtactgttg
    12541 ttcctctgcg gttggttcca ggggatgggg tggccgccgt gtggtcgtac tatggtgcac
    12601 tggtggtcgc agaaagaacg tggcggcatt gtgtcagtgt ggaactgtgc gcacaacgtc
    12661 ggtggtggta ttccgccgct gctgttcctg ctggggatgg cctggttcaa tgactggcac
    12721 gcggcgctct atatgcccgc tttctgcgcc attctggtgg cattattcgc ctttgcgatg
    12781 atgcgcgata ccccgcaatc ctgtggcctg ccgccgatcg aagagtacaa aaatgattat
    12841 ccggacgact ataacgaaaa agcggaacag gagctgactg cgaagcaaat cttcatgcag
    12901 tacgtgctgc cgaacaaact gctgtggtat atcgccatcg ccaacgtgtt cgtttatctg
    12961 ctgcgttacg gcatcctcga ctggtcaccg acttatctga aagaggttaa gcatttcgcg
    13021 ctggataaat cctcctgggc ctacttcttc tatgagtatg cgggtattcc gggcactctg
    13081 ctgtgcggct ggatgtcgga taaagtcttc cgtggcaacc gtggggcaac cggcgttttc
    13141 tttatgacac tggtgaccat cgcgactatc gtttactgga tgaacccggc aggtaaccca
    13201 accgtcgata tgatttgtat gattgttatc ggcttcctga tttacggccc tgtgatgctg
    13261 atcggtctgc atgcgctgga actggcaccg aaaaaagcgg caggaacggc agcgggcttt
    13321 accggtctgt ttggttacct gggcggttcg gtggcggcga gcgcgattgt tggctacacc
    13381 gtggacttct tcggctggga tggcggcttt atggtaatga ttggcggcag cattttggcg
    13441 gttatcttgt tgattgttgt gatgattggc gaaaaacgtc gccatcagga attactgcaa
    13501 aaacgcaacg gaggctaatg gcatgaaatt gacgctgaaa aaccttagca tggcgatcat
    13561 gatgagcact atagtcatgg gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt
    13621 catcgcccat cgcggtgcca gtggatattt gccggagcat acgctgccag caaaagcgat
    13681 ggcgtatgcg cagggagcgg attatctgga acaggatttg gtgatgacca aagacgacca
    13741 tctggttgtt ctgcatgacc attacctcga tcgtgttact gatgttgccg atcgtttccc
    13801 ggatcgggcg cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg atgaaattaa
    13861 gtcgttgaaa tttaccgaag gtttcgatat tgaaaacggt aaaaaagtgc agacttatcc
    13921 agggcgtttc ccaatgggta agtccgactt ccgggtgcac acctttgaag aagagattga
    13981 atttgttcag gggttaaatc actctaccgg gaaaaatatc ggtatctatc cagaaatcaa
    14041 agcgccgtgg ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc tggaagcgct
    14101 gaagaaatat ggttacaccg gtaaagacga taaagtttat ttgcaatgtt ttgatgctga
    14161 tgagctgaag cgtattaaga atgagctgga acccaaaatg ggcatggatc tcaatctggt
    14221 acagctgatt gcctataccg actggaacga aactcagcag aaacagccgg acggaagctg
    14281 ggttaattac aactacgact ggatgtttaa gccgggtgcc atgaaacagg tggcggaata
    14341 tgcagatggt attggtccgg attaccatat gttgattgag gagacatcgc agccgggtaa
    14401 tatcaaactc actggcatgg tgcaagatgc tcagcagaac aagctggtag tgcatcctta
    14461 taccgtgcgg tcagataaac tgcctgaata cacaactgat gtgaatcagt tatatgatgc
    14521 tctgtataac aaagcgggtg taaatgggtt gtttactgat ttccctgata aagcggttaa
    14581 attccttaat aaagagtaat gctgctactg caggatggcg ttcatgcgcc atcctctgct
    14641 aaagcgaaat aatgctacta ccggttattc ttttcattaa ccggtgcatc ctgtaaaaaa
    14701 gatgccaaca taattttcgt aaaactcatt cgttaaaaaa gctaacactg ggcattgt
//
LOCUS       DAZUCE010000120        14670 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZUCE010000120 DAZUCE010000000
VERSION     DAZUCE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.120"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            141..1070
                     /gene="kdgK"
                     /locus_tag="ACHY03_003097"
     CDS             141..1070
                     /gene="kdgK"
                     /locus_tag="ACHY03_003097"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFS3906297.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICAELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(1166..2662)
                     /locus_tag="ACHY03_003098"
     CDS             complement(1166..2662)
                     /locus_tag="ACHY03_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS3906298.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(2883..4169)
                     /gene="dctA"
                     /locus_tag="ACHY03_003099"
     CDS             complement(2883..4169)
                     /gene="dctA"
                     /locus_tag="ACHY03_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFS3906299.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(4352..6340)
                     /gene="hmsP"
                     /locus_tag="ACHY03_003100"
     CDS             complement(4352..6340)
                     /gene="hmsP"
                     /locus_tag="ACHY03_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFS3906300.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(6422..9895)
                     /gene="bcsC"
                     /locus_tag="ACHY03_003101"
     CDS             complement(6422..9895)
                     /gene="bcsC"
                     /locus_tag="ACHY03_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFS3906301.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKSAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKVIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(9877..10983)
                     /gene="bcsZ"
                     /locus_tag="ACHY03_003102"
     CDS             complement(9877..10983)
                     /gene="bcsZ"
                     /locus_tag="ACHY03_003102"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFS3906302.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(10990..13329)
                     /gene="bcsB"
                     /locus_tag="ACHY03_003103"
     CDS             complement(10990..13329)
                     /gene="bcsB"
                     /locus_tag="ACHY03_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFS3906303.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAIAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(13340..>14670)
                     /locus_tag="ACHY03_003104"
     CDS             complement(13340..>14670)
                     /locus_tag="ACHY03_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFS3906304.1"
                     /translation="FCGSCAVIRRKPLDEIGGIAVETVTEDAHTSLRLHRRGYTSAYM
                     RIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPLTGKGLKFAQRLCYVNAMFHFL
                     SGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPHMIHASLTNSKIQGKYRHSFWS
                     EIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVEEEYVDWVISRPYIFLVLLNLV
                     GVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGAVAVSVESKQVRRSHRVEMTMP
                     AAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQKVNLLLKRGQQEYVFPTQVAR
                     VMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQDSYPEDKPLESLLDILKLGFRG
                     YRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRPERSETAQPSDQALAQQ"
BASE COUNT         3377 a         4153 c         3828 g         3312 t
ORIGIN      
        1 aatttcccct ctccacaacg gaatctttca ggaatacgtt ctggctcaaa aaaataatgg
       61 aacactgttt taatatggtt gaccagcaaa ccaccacagc gcaaactaac gctaattttt
      121 tacagatcag gttcacgact atgtccaaaa agattgccgt gattggcgaa tgcatgattg
      181 agctttccga gaaaggcgcg gacgttaagc gcggtttcgg cggcgataca ctgaatactt
      241 ccgtctatat cgcccgtcag gtcgatcctg cggcattaac cgttcattac gtaacggcgc
      301 tgggaacgga cagttttagc cagcagatgc tggacgcctg gcacggcgag aacgttgata
      361 cttccctgac ccaacggatg gaaaaccgtc tgccgggcct ttactacatt gaaaccgaca
      421 gcaccggtga acgtacgttc tactactggc gtaacgaagc cgctgccaaa ttctggctgg
      481 agagcgagca gtctgcggcg atttgtgcag agctggcgaa tttcgattat ctctacctga
      541 gcgggattag cctggcgatc ttaagcccga ccagccgcga aaagctgctt tccctgctgc
      601 gcgaatgccg cgccaacggc ggaaaagtga ttttcgacaa taactatcgt ccgcgcctgt
      661 gggccagcaa agaagagaca cagcaggtgt accaacaaat gctggaatgc acggatatcg
      721 ccttcctgac gctggacgat gaagacgcgc tgtggggtca acagccggtg gaagacgtca
      781 ttgcgcgcac ccataacgcg ggcgtgaaag aagtggtggt gaaacgcggg gcggattctt
      841 gcctggtgtc cattgctggc gaagggttag tggatgttcc agcggtgaaa ctgccgaaag
      901 aaaaagtgat cgataccacc gcagctggcg actctttcag tgccggttat ctggcggtac
      961 gtttgacggg aggcagtgcg gaaaacgcag cgaaacgcgg gcacctgacc gcaagtaccg
     1021 ttattcagta tcgcggcgcg attatcccgc gtgaggcgat gccagcgtaa acgcagcata
     1081 tgcaaaaggc tggataaagt gttacccctc tatccagctc atccataaat acctgatggt
     1141 tgtgctaact catcaccatc aggcattact gtgcaggtgg aatatccgtc acttcaggat
     1201 gtacgtcatc cgtggcgaca gaggtggtcg cagcggcggt agatggggcc atgatttgat
     1261 cccaggccgc ctgcaacgct ttcatgttaa attccggctc gcctttcggc tgcagcagta
     1321 ttaacgccat atcattcgac aactgctgac gcagatcctg atttaacatc tccacagtca
     1381 ggctgttaag gaaatcctgc cgcagtttct gatactgctc cggcgcgata tcgacgacct
     1441 gattttgcaa cgaacgcatc cgctgaccca tcagaatatc ggtatcagct cgtgcatagg
     1501 cggcaaacag tttctgcagc tccagtttct tttgcgccac taacgcattg aactcttctt
     1561 ccggcagacc tttatcgcga accttcgcca gttcacgcgc caccagatta aggttgctgt
     1621 tcagcttgtc gtttggtgat tcgatgttga tggcacactg cgcacgcaga tacagcacac
     1681 ggcagtcaaa tccaagaccg atgtctttgc tgttactggc gcttaacgct tgctgaacat
     1741 gccagaacag cgcctcacgg gccaggtccg cacgccagta gcgcagcagt gcggctgatt
     1801 cacgaatcgg ctgccacggc gtatcccaca tgatagataa ccggtcctga cgcaccgcgt
     1861 cagtcataat actcaccgct tccgcacgca gcggagaaag cgtcggcacc ggagctggcg
     1921 tttcacgttt gcctttcagt tcgccaaacg ttttgttgat ttggtcgaca accgagcgcg
     1981 catccacgtt tcccaccacc agcagcgtca ttgcatccgg ggtgtaccat ttctgataga
     2041 aatctttaat tttttccgct tcaacgggtt gtttcagcgg atcggcagga tcgtgaccta
     2101 acagggttga tcctttcaga cgataacgcc accagccctc tttagtatcg gcaggccagg
     2161 ttgccaccat gtcctgactt tgcagcgcgt ggttgatggt ttctggcgtg atagtcagtt
     2221 tgccagtggc atttgccaga taagagagcg cctctttcag caaatcgtta cggttattgg
     2281 gcaaactcag attaaacagc gtggtgtcat aagagacaat taccggcggc atcgggcgtt
     2341 tagggtcgat cccctgctgc cacaatgaac gcgcctgtgc tgcgtcaagg ccaccgcttt
     2401 gcgttagcgc aatacgaggg atggcgtgac tgtaaccgct ctgttgtgta ctttcggcga
     2461 gcgaaccggt attaaccagc aggcgaattt caacacgatc gctgggacgc tggggggtgg
     2521 tcagcacttg ccactgtaaa ccgttggaaa gcgtcccctg ttgccatgct ggatcaggct
     2581 ggagcgcatc tgcctgcaca tagccagcag tggccatcat cagcaaaccg cccgctaaaa
     2641 gtcgaatttt tgtgccctgc atgtgaaccc ctgatcaact atcctggtaa taaaaaaact
     2701 gcccgttgtc ggtcagtcta aatgacgtta aaaacacttc gtgttagacc gcaagaacat
     2761 gaaaatgtca cggaagaagt gaaataaacc tgaactcacc caggtggtaa gttcaggcac
     2821 aggggtcaat tatgcgcaaa cacccgcact cggggaaggg agtgcgggca taagtgatga
     2881 gattaagagg ataattcgtg cgttttgcca tccggcgcac gattattcag cacatcgtcc
     2941 agttttttgt ggtccagttc tttcacccac ttagcaacga caatggtcgc tacgccgtta
     3001 ccgaccaggt tagtcagcgc acgagcttct gacataaagc ggtcgatacc gaggatcagc
     3061 gccagacccg ctaccggcaa atggcccaca gcagagagcg tcgccgccag cacgataaag
     3121 ccactacccg ttacccctgc cgccccttta gaagaaagca gcaacacgat caacagcgtg
     3181 atttggtgga cgatatccat ctgactgtta gtggcctggg caataaacac cgccgccatt
     3241 gtcaggtata tcgatgtgcc atcaaggtta aacgagtagc ctgtcgggat gaccagcccc
     3301 accaccgatt tacggcagcc gagtttctcc atcttgtcga gcatacgcgg cagcgccgac
     3361 tcggaagatg aagtccccag tacaatcagc agttcttcac ggatgtagcg gataaatttg
     3421 aagatactga aaccagtcgc tttagcgatt gaacccaata ccagcaccac aaacaggata
     3481 caggtaatgt agaaacagat aatcagctgc cccagttgca ccagtgtgcc gacgccgtat
     3541 ttaccgatgg taaacgccat tgccccgaac gcaccaatag gtgccagacg catgatcata
     3601 ttgatgatgc cgaagatgac ctgcgagaaa ctttcgatca cgttaaaaat cagttggcct
     3661 ttgctgccca gacggtggag cgcaaaacca aacagtacgg caaacagcag cacctgcaga
     3721 atgttaccgc tggcaaatgc gccaatgacg ctcgccggga tgacatccat aatgaaggcg
     3781 acaatgccct ggtctttcgc ctgatcggcg taaaccgcta ccgctttcgc atcaagcgtt
     3841 gccggatcga cgttcattcc ggcaccaggc tgcacgacgt taacgatgat aagaccaata
     3901 atcagcgcga tggtactgac aatttcaaag taaagcagtg cgactgcgcc ggtacgaccg
     3961 accgccttca tgctttccat gcccgcgatg cccgttacga cggtacaaaa gatgacagga
     4021 gcgatgatca tcttaatgag cttaacgaag ccgtcgccaa gcggtttcat ttgctcgcct
     4081 atttcaggat agaaatggcc aaggagaata ccaatggcta tcgctgtcag gacctgaaag
     4141 taaaggcttt taaacagaga ggttttcata gggtgtcctt tagtaaaacc acaggtcttg
     4201 taaggttatg gggtacctgc ggccttaaaa taacacccag acaacatcac agaaatgtac
     4261 ctggatcata attgaaacaa aaaggttaaa aagtttgagc tggctcgcac aaaccagcac
     4321 tttttaaagt tttgtaatca gtttggggta gctacttttc ttccaggtaa ctctcttcga
     4381 agatttcaat agggagtggg cgagcaaaaa ggaagccctg ggcaatacca acgcccgctt
     4441 ttgccagcca gtcgcgttgt gcttcagtct ccacgccttc ggcaatcatt tgtaagttca
     4501 ggctctgcgc cagcatgata attgcagcaa tcatgctgct atcttccggc aagccttcaa
     4561 caaacatttt gtcgattttc agaacgtcga ttggcaacga tttcatatgc tgcagctgac
     4621 gcagccctgc gtagcccatg ccgaaatcat ccagcgccac ccgaactccg gcattgcgca
     4681 gcggacggag gattgccacc gcagcatgag ggtcgtcaat acgtcggctt tctgtcactt
     4741 ccagaatcag tgttcccggc tgaatgcgat agcgggttaa cagttccagc atatccgcca
     4801 ccatattcgg gtgcatcagt tgcagcgcag agaggtttac cgacaagggc agcataatgc
     4861 cgcgctcttg ccaggcagcc aacagtcgac aggactcttc cagcacccag tgaccgacgg
     4921 taaccatcag cccacagcac tcaatgcgat cgattaagcc atccggcagg tcccaactgc
     4981 catccggttg ctggatacgc agtaacactt ccgcactgac cagtttaccg ctggtcatct
     5041 cgacctgtgg ctgtaaccaa atagcaaact gatgattttc cagtgcatta aggatatcgc
     5101 tctcttccgt caaccgcttc tgggcggctt ccatctgctg cggatcaaag aactgaatct
     5161 gattcttgcc tttatggcga gcggtaaatg ccgcagaaat agcgcgactg taaagctgtt
     5221 cggcggtgag atcgccgtag aacatcgcca cgccaatgct acagtgcgga cggagttgaa
     5281 tacgttcaat cggcaggcgc tcgctcatga tagtgagcac ttgctgacct aaggtgattg
     5341 cgtgccacgg ttcctgtaca ccattggcaa tgacagcaaa gtcataaccg ctaatctgcg
     5401 cgaggatcat acgtggcgac agtaccgatt tgagtttttc caccagcgtc agcagcagaa
     5461 tttctcgttg cgcctctttc agcacacccg cagtatcacg cagggtttca caggtgatga
     5521 tcatcagcgc ggtggtttgt ttacgcgcga caacctgctc cagcatctcc atcagcaagg
     5581 ctttgttcgg caaatccgac actgggaagc gcatcgcatt ttcgttctgt tcttcataat
     5641 ggcgctgcag caattgctgg ttgaggttgt aactgcgcac caacataccg atttcatcat
     5701 cctgatgcag acgcggtaat gccagttggt gaccaacaag ctcctgggct gggatggcgt
     5761 taagttcgcg agcaatattg cgtaacggat gcaaaatcag gcggttaatg caccagctaa
     5821 tcgccaccgt caatattaac gacaaaagta agtaaatggt cactaacgtt gagagggtgc
     5881 tcatcacgaa cttatacata cggaaggaat ccgcctgtag caccagatag gcaattggct
     5941 gcggatttgc cggacgttcg agcgagtaga cgcccagcga gatttgaacc ggtagctcaa
     6001 acaggcgagt aaccattacc ggcaccgggc gctctggaat aaaactttta cgcagcgcct
     6061 ggaactggtt gggcagcact acatcggcac ggctgaccac gcctgccggt ttaatgctgg
     6121 caagaatagc ttccgcttcg ggaatatcgc ctttcaaaat ggctgaagat aaaggttcgc
     6181 ggacagagcg agcaatgctt tccagttgcg tagccgtgtt atagcgattc tgctggacca
     6241 gatggaacag caaaacggtg caaaaaataa aaacgaacac caggacaacg gctgccacca
     6301 ttgccatctg cttgattgtt aacgagcgac ttacgcgcaa attgtctccc cccggaaatc
     6361 ccagattgcc gcccgagtat acccgatcgc agcggcatta agagaggcgc tatctgaaaa
     6421 cttaccagtc ggcgtaaggt atcagcggct gcggcggtaa atccatgtca ccctgccatc
     6481 cggcggcgga ataacgtacg tagagcagga aatggctggg tgcgtaatct ttcgcctgct
     6541 ggatatcaat tgccgtgccg acaaaccagt tggatgtgac gcgacgctca agtaatgccc
     6601 gcgccgtata gccaaagccc tgactgctgc cgccatcgtt ggattgccgc gcagcttctt
     6661 cctgccagtc agtcgggatc agattcatca gcggataacg cggcatggtt ttggtgcgtg
     6721 aatgcgacca cgagccagac gcccccagct cccacgacca gttttccgtg cgctcccgcc
     6781 acatcaccgg tatggcaaac gacaggtatt cctgcgggct gtagtaaccg ccctgaccga
     6841 gtgagtagcc actcaggtct ttgtcgtaat gccagatcat gttgttcagg ccgattgtga
     6901 cgcggcgatt gttctggttg atgaccttat agtaatagcc cgtcatccag cgcacgcgcc
     6961 agttatcttc gacattttta ccggttaact ggtcgccact aagcgatgcc cagacgccgt
     7021 ttgcttcacc tttatcgtag ctcaggctta gccccacgcc gtcggcacgc acaccgcccc
     7081 attttttccc ggtattgctc ggggagtctt tttgcccacc aaaggccagc aaagaactgg
     7141 agatgggccg acggtgggcg ttaacggtgt aacccagcgg cccgatatca tcgctgtaac
     7201 tgatgccgcc gaccacatcc accacgttga agcccatcgg cgtggtgccg atatcccagc
     7261 tccagacgtc atttcgccag ccgaccgcca cgctggcacc ggaatccgac tggctgcggt
     7321 tgccgctaca gtcctgtaat gtacaggtgc cccagttatc atcccattta ccatcggcat
     7381 tagtggagaa actgccgacg ttcatattga cgaaatcact gcgaaagaac atccgcccgt
     7441 cagaatacgg cgcatccacc tgcaacatgg tagtgtgcgc tttcagatcg gagtaaccac
     7501 cggtgccgct cgaaccccaa taatcatgct cgagggtgac gttaagatcc tgctggcgat
     7561 agaggtccgc cgcatcgctg cgcacgccgc gtttcagcca gtcatctttc tcgtcgttac
     7621 gggtcagtcg ggtaaaggtg tcgttatcct gcggacgcgt cgtagtcaca ccggatgcga
     7681 ccatggcgtc tttgtaggtt tccagcgcct gctttggatc gcccgcctgc gcttcaaact
     7741 tcgcaccatc acgcagtacc atcgcacttt ccatcgacgg tggctgagat tttgcctgcg
     7801 ggatcacctt attaaacgtc tgctgcgccg ctgcggtatc gccaagctgc gcctgcgcca
     7861 gcgccacgcg ccgctgtgtg ttcagcgagg cgttatcggt ggcaggcagt ttcgccagct
     7921 ggctacgtgc cgccgatttg tcaccagcag caatatccac ttccgtcaga ccaagaatgg
     7981 catcggcgtt agttggctcc cgcgtcagga cattctgata tgcagcgcgg gcggcggtgt
     8041 aatcacgtcg ctgctgcgcc cagtcagcca gcgtgaggtc aatacgcgtg gaaggtggtt
     8101 gctgacgcag catcgcttcc gcttcagctt ctttgccgct ttctcgcagg cgattagcgg
     8161 tttccagcac ctgatcgctt tgcagtcgat taaccagctc ctgaatattg ctgctccact
     8221 gcgcacgcgg caggctgttg atatgcgcca gcgccgctct gtcctggtca tgaccagaga
     8281 ggtacagccc gtaagcgtaa acctgctccg ggtcgttcgg cttctgctgc gccagattgc
     8341 gcattaacgt atcggcctgg ctgcgttgtc cggcctgcca gagatcctgc gaaagtcggt
     8401 aagtaatcca tacgctgccg gggtccagcg ccagtcgttg ccgctgaagt gctgccgcct
     8461 gcgcccattt gccctggttt tccagtgcct ctgcctgctg tgccagacgg tcgttttgca
     8521 ggctgcgttc gatatcatca atgctacgcc gctgactggc agagagcgag gcgataaacg
     8581 cttcagcttt ttctggcgat tgctggcggt aaatatttgc cagcccgcgc acggcgttag
     8641 tgttgccgct gtccatacgc aaggtctgct gataataacg ttcggcggcg ggataatctt
     8701 ttcgcgccat cgccacatcg cccagcccca gcactgcata actgtcggtg ttatcgacat
     8761 tacgcgcctg ctggaacagg cgttctgccc ggtcaggatt attggctttc agcgcagcat
     8821 cgccctgctg gattgccagc cagtagcggt tcactttcag cagactgttc catttgtcgt
     8881 tattgctgtt gtgcggatcc agcgcgaggg ctttttccag attcgccacc gcattggcgc
     8941 gatcgccttt ctgagaatac gcctggccca gcgcccccag agcctcactg tctttcgggt
     9001 tcgcccgcac tgcctgttgt agttcgggaa tggctttacc cgccatacca gagtccaccg
     9061 ccgctaaacc ttgcgcacga gcgcggaaag caggatcggc cagctgtttt tgctgttctg
     9121 ccagttgcga ttgcgcagcc gccacgctat cgccatcact aaagatcgaa agatattttt
     9181 tcagcgcctg cacgctggca tcgctgacgg gcatgtcttt aatctgcccg taccagattt
     9241 tagaggcccc ctcgcgtccg gcgttcgatt tcgccatttg ttccagaacg gcaaagcctt
     9301 cgtcacgccg atcgctacta aacagcaata gcgccagatt gttttgcagg cccgtgttgc
     9361 ccggtgcatc cgcattgatg cgttttaact gattgatcgc ttcgccacgg cgagccggaa
     9421 ttttcgccac cgtactccag tactcgacag caatgtcacc ttccggcggc gcaccgttga
     9481 acagtttgtt gtaactcgcc acagcttctt ccgcatgacc ggtagtcgcc tgcaatcttg
     9541 cctgttgcag tgcctgacga ccatccggcg tggaaagtag catcgtagtc cgcgacgatt
     9601 tatacgcatt tgaactcggc gctaactgcg acagccgatc gagctgtttt tgcgcgccat
     9661 caatatcgcc ctgacgtaac aaagaacgga aacgggcggc aacgacgtcc gggttattcg
     9721 gatcgataag ttccagccga tacaacgact gctgcaccag gtcttcacga tgggtcgctt
     9781 cgcctaaccg aacttgctcc agcaactgtt gctgagcggt tggtgctgcc tcgaccatcg
     9841 gcatgacggc cagaccgagg gaaagcgtga atatgtttag tgtgaatttg cgcattcctg
     9901 gccccagtca ggtaataact caccttttgt cgagaagcgg aaacggtgtt gatcccagcc
     9961 ttgtccaaac agggtcagca catagttgta ataggcatcg ctgccgggaa agttatcggc
    10021 cacgcgctgg cgctgaacgg cctgcgcatc acggttttgt aaaaagggca gcatggcggc
    10081 agaaaaaccg accggtcctt taccctgcgc tttccccgta gccacatcca ctttttccgg
    10141 cggataaccg tttttctcag tgaatgtcgc catcggttta aaccggttga gcatccgcgc
    10201 tttctgcgga tcgctgtcag gcatcatgcc tacccacatg taaacgcgga tagcgtcgta
    10261 gctgctgatc aatgtttttt cggcttttag ctgccagcct ttgtctttct catagcgcac
    10321 ccagtctggc gaaaagcctt tcggggcggt ttccagcaat aaacgttgat tggtttcgcg
    10381 cagcgtagtc cacggcgcgc caaagcgggt gaaatactgc gccagcgtcg gcggcaggta
    10441 gctggggtta aaacgccagc tgttatcctc agcaaaaccc actttgcccg gtaacaacat
    10501 ggaacccagc ccaggcaccg tcaccacttc ctcccgcgca atacgtttta gcaacgcgct
    10561 gccgatgtcg gtataacgct gctctttcca caaacgcccc gcctccagca acgaccaggc
    10621 catccagaca tcaccatcgg aggccgaatt gctgtccagc acttcccact tactgttctc
    10681 tttcttgccc cacagccagg cgggcaaacg ttcttttaaa gaaccctgag cgagattgtt
    10741 ctgcgtccag tcgagaatat tatcgaaagc tgcacggtcg ttagccgcca gggcaaagaa
    10801 catgccgtaa ctttgccctt cagaggtggt gattttgcgc gcgtcgctgg ggtcgatgac
    10861 gcgcccttcc tgactgatgt aatccttttt aaactgctcc caggcaggcc aggtacaggc
    10921 tgcctgaaca ctaaaggcag ccaacagcag catcgtcacg agtccactac gcaacacatt
    10981 catcttcaat tactcgttat ccgggttaag acgacgacgg ctgataatac gcagcaaacg
    11041 ccacagtacc catgccagca atatcacact gatagccgcc agcaccgcca gcagaatcgg
    11101 atggtttgcc agcgcatacc acaagcgctc gaaccacggc agatgaccta cgtaataaac
    11161 gtcgccaaca cgtaggctgt tgataccgga ctcgcggatc accgcgaccg aaccgaacat
    11221 ggtggcgcgt ttgccgctat cgttcaccgc atcgttaagc atttcataac cgcgtgggct
    11281 atctgccagc agcgcaatca cgctgcgctg gtcgttatac ggcgactgga agccaatcac
    11341 cgccgccatc gcaccggaag aggtcagcgt tgactgggtt tctgccgcgc gatcgctctc
    11401 gtccggcaca atgccgggga acggggtctg gcgcatcggt gttttcaccc agctttcggt
    11461 cgcctgcacc aataggtcga tctgcttatc gtctttcagt ttgtccggga taccaccgat
    11521 gatcatgatg tcggcatctt tgccctgaat ggtgctgcca tcatcggtca ccgtcagatt
    11581 aatcgccggg aagcccgtct gtgcgccgat aaaaccaaca gtattcagca acgtttccat
    11641 ctgtgcttcg ttaggcgttt tcggcatcac ggtgatggtt tgcgacagat ccgccatccg
    11701 gctgaacggg aagcccgcgt tagcaaaggc gcgtagatcc ggcatcggga tgaagtggta
    11761 atacttcgag aagtcgatgg tggagtcgtc accaatcacc acatgattct gcaccggctg
    11821 gaaggtaata cagttatcca ccgaaccgcc cggcatcggg ttcatatact caaagtcgaa
    11881 gcgcagctgg ttggtcgcgc ccagtttcag cgccggaata gagacatccg ttttgccatc
    11941 cagtaaacct tgtaataccg gaatccgcag cagcaggcgg ttcgcctcct gtttgctgct
    12001 caggttgaag gattgcagga actggttatt caggctgata tccatccgcg aactgtcttt
    12061 caccggcggc atggtgtagc ggtaattaat atccatatca atgccggtac tgcgcatcag
    12121 gtagagatct ggcggcaggt ttagcgaaac attaatcgct gctggctcaa gaccgctgga
    12181 ttgtaactgt tcttcatagg ttttcagttc accaaaggtg accggacgat cggtacgcac
    12241 ccagttcggc gcatcgtacg gcttacgcgg tagcagcggt ttcacttcat tcactaccac
    12301 gctttcacca cggaacagga tgttaccctg agcgatacct ttcgctgcct gcaacaggtc
    12361 tttgtcgtca cgaccaaaca ccaccagcag tttgacgtaa ggattctgcg gatggttgat
    12421 catctcaatc accggggctt ttaccgccgg atgatcgcgc aggaagtccg gccgtttgtc
    12481 gttggttgca aagacaatgg cattacgatc cggcagttgg ttatagagca ccgggaagtt
    12541 ctgcccacgc cagccagaac gcgaaccaaa ccacgaggcg acaatggcag atgcttgttg
    12601 cagcccaaca tccggcgcac ccgcaaagac catcggcaag gtgttagtac ggttatcacg
    12661 cgggtcaaag aatggtaccg ggaagtgtga caggtcattc ttcacattca gggtctgata
    12721 ggtcaaatcc agtccactgc tccgcccaac atccagccaa agcgtggtgc tggccgggtt
    12781 ttcgcacacg tcctgataat ggccgacaaa ctccagccgc acacggttga agtcggtaat
    12841 aaacagtggg ttaatgggca tttgcgccag cgttttttta cccaactgtt ctttggtcac
    12901 tggcagcacg cccatcagct catcattgag ataaaccttt aactgcgact ggacaggcag
    12961 taacgatggc gatggcgtgt attcgaggtt gagcatcgcc ttcgtcacca cttcatcgct
    13021 gcgcatacca aactcaatgc tgccgttcgg gttaatgcca cgtagcacca tgctgcccgg
    13081 cggcggcgca atttgcgcaa aggtcagctt cacatcacgc gaaggaccgt tctgcgccac
    13141 gactggcgca tcagcgccct gcacgcccgg catcacttgc cccacctgcg gattttgttc
    13201 cgtctgggcg gctatagctg gctcagcatt gatcagtggt tgcgttgctg gcgtcgcctg
    13261 cgtcatgaaa gagggaaacg cactcatccc catagccact gcacaaatcc agaatagttt
    13321 tcttttcatc gcgttatcat cattgttgag ccaaagcctg atccgatggt tgtgccgttt
    13381 cgctccgctc cgggcggcgc ggaataaacg atacaaccca ggaaaccaga gaagtcagca
    13441 cacggaatat gcccttcacc gaagaaggcg caaactccgc cagatggcgg tagccacgga
    13501 agccgagctt cagaatatcc agcagacttt ccagcggctt atcttccgga tagctgtcct
    13561 gccagagcgc ccatgtatcc gcacgggcaa acgtacactg cacaaaatcg atatgttgct
    13621 gggtggtgag cggcattaat ttcagcccaa cttcattacc catcacgcgc gccacctggg
    13681 tcgggaagac gtattcctgc tgaccgcgtt taagcaacag attcactttc tgcccttcca
    13741 gaatctgcgc ctgaccgttg atcttgatcc ccaaaccacc gtcggagaaa tcctgaacgg
    13801 tacacgaaaa gaggtgacca tcttcgcggg caattgccgc gggcatcgtc atctccacgc
    13861 ggtgcgatcg gcgtacctgt ttgctttcta ccgatactgc aactgcgccg ccaagaacaa
    13921 tcaggttgta gaatacccac accatactga cgaccacggt gagcatctcg gttggcgggc
    13981 catagaagta gcgccagatg cctaccgcaa cgcccaccag gttgagcagg acaaggaaga
    14041 tgtagggccg cgagatcacc cagtcgacgt actcttcttc caccagtcca cctttggcgg
    14101 tgacgttaaa tttgcctttg tgcgggttaa tcagcgccac cagcgtcggt ggtgcgatat
    14161 accacgccag caccgtttcg tagatttcac tccagaaaga gtggcgatat ttgccctgga
    14221 tcttggagtt ggtcaggctg gcatggatca tatgcggcag cacgaatagg gcgatcatca
    14281 gcgctggcgc atagatgatg taggcatgaa gcagcaggaa cgccagcggc gcagtcagga
    14341 agatcagccg tggaatgccc gacaagaagt ggaacatggc gttgacgtag cacagccgct
    14401 gagcaaactt cagcccttta ccggtgagcg ggttatcgag acggaagatt tgcaccatcc
    14461 cgcgcgccca gcgaatacgc tgaccgatat gcgccgacag actttcggtc gccagccccg
    14521 ccgcctgcgg aatacgcata tacgcggagg tatagccacg acggtgcaaa cgcagagaag
    14581 tatgcgcatc ttcagtcacg gtttcgacag caatgccgcc aatttcatcc agcggcttac
    14641 ggcgaatcac cgcacaggaa ccgcagaaga
//
LOCUS       DAZUCE010000121        14666 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZUCE010000121 DAZUCE010000000
VERSION     DAZUCE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.121"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(5..112)
                     /locus_tag="ACHY03_003105"
     CDS             complement(5..112)
                     /locus_tag="ACHY03_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS3906305.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            588..1859
                     /locus_tag="ACHY03_003106"
     CDS             588..1859
                     /locus_tag="ACHY03_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFS3906306.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1889..2893)
                     /gene="dppF"
                     /locus_tag="ACHY03_003107"
     CDS             complement(1889..2893)
                     /gene="dppF"
                     /locus_tag="ACHY03_003107"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFS3906307.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQTLLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2890..3873)
                     /gene="dppD"
                     /locus_tag="ACHY03_003108"
     CDS             complement(2890..3873)
                     /gene="dppD"
                     /locus_tag="ACHY03_003108"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFS3906308.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3884..4786)
                     /gene="dppC"
                     /locus_tag="ACHY03_003109"
     CDS             complement(3884..4786)
                     /gene="dppC"
                     /locus_tag="ACHY03_003109"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFS3906309.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4796..5815)
                     /gene="dppB"
                     /locus_tag="ACHY03_003110"
     CDS             complement(4796..5815)
                     /gene="dppB"
                     /locus_tag="ACHY03_003110"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFS3906310.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5966..7573)
                     /gene="dppA"
                     /locus_tag="ACHY03_003111"
     CDS             complement(5966..7573)
                     /gene="dppA"
                     /locus_tag="ACHY03_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFS3906311.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(8326..8402)
                     /locus_tag="ACHY03_003112"
     tRNA            complement(8326..8402)
                     /locus_tag="ACHY03_003112"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8366..8368),aa:Pro,seq:cgg)
     gene            complement(8494..10185)
                     /gene="eptB"
                     /locus_tag="ACHY03_003113"
     CDS             complement(8494..10185)
                     /gene="eptB"
                     /locus_tag="ACHY03_003113"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFS3906312.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAVAN"
     gene            complement(10440..10970)
                     /gene="lpfE"
                     /locus_tag="ACHY03_003114"
     CDS             complement(10440..10970)
                     /gene="lpfE"
                     /locus_tag="ACHY03_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFS3906313.1"
                     /translation="MKFNLLLHSGIASLSLFACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            complement(10975..12030)
                     /locus_tag="ACHY03_003115"
     CDS             complement(10975..12030)
                     /locus_tag="ACHY03_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3906314.1"
                     /translation="MKAAIALSLLGCVFGFSGKAFAGDAWGPCTPADGTTYHYNVDVD
                     VGIPDAAKNVAGTVLPDVLNWSNGQNVSLICECPDSYKNEKDTLVQGVSMLPPSGRSV
                     DSMKYYTLTEELEVATKIRISTSVYGFVPFKNQQALQTTGCNKVITTPYMGGAGLLSF
                     AITKPFIGDSVIPLTLIAELYASKTNKDYGTIPISSVSIQGRVTITQDCEIKPGTVLD
                     VPFGEFPSSAFKNRQGQMPEGATEQEINLGFDCNNISDGIKVSLRLEGATNADDPRAV
                     DMGNPDIGVLVKDSSGKILVPNDASSTTLLNLSSLDSKTHRNAAIRLLALPISTTGKA
                     PKGGTFEGVTTIYLEME"
     gene            complement(12045..14618)
                     /gene="lpfC"
                     /locus_tag="ACHY03_003116"
     CDS             complement(12045..14618)
                     /gene="lpfC"
                     /locus_tag="ACHY03_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFS3906315.1"
                     /translation="MSRKTVSRTFSSFSISVVAVAVASTFSAHAGKFNPKFLEDVQGV
                     GQHVDLTMFEKGQEQQLPGIYRVSVYVNEQRMETRTLEFKEATEAQRKAMGESLVPCL
                     SRTQLAEMGVRVESFPTLNQVPAEACVPFDEIIPQASSHFDFSEQKLVLSFPQAAMHQ
                     VARGTVPESLWDEGIPALLLDYSFSGSNSEYDSSGSSSSYVDDNGTVHHDDGDDTLKS
                     DSYYLNLRSGLNLGAWRLRNYSTWSHSGGKAQWDNIGTSLSRAIVPFKAQLTMGDTAT
                     AGDIFDSVQMRGAMLASDDEMLPDSQRGFAPIVRGIAKSNAEVSIEQNGYVIYRTYVQ
                     PGAFEINDLYPTANSGDLTVIIKEADGSEQRFIQPFSSVPIFQREGHLKYSFAAGEYQ
                     AGNYDSASPRFGQLDLIYGLPWGMTAYGGVLISNNYNAFALGIGKNFGYIGAISIDVT
                     QAKSELNNDRDSQGQSYRFLYSKSFESGTDFRLAGYRYSTSGFYTFQEATDVRSDADS
                     DYNRYHKRSEIQGNLTQQLGAYGSVYLNLTQQDYWNDAGKQNTVSAGYNGRIGKVSYS
                     IAYSWNKSPEWDESDRLWSFNISVPLGRAWSDYRVTTDQDGRTNQQVGVSGTLLEDRN
                     LSYSVQEGYASNGVGNSGNANVGYQGGSGNVNVGYSYGKDYRQLNYSVRGGVIVHSEG
                     VTLSQPLGETMTLISVPGARNARVVNNGGVQVDWMGNAIVPYAMPYRENEISLRSDSL
                     GDDVDVENAFQKVVPTRGAIVRARFDTRVGYRVLMTLLRSAGSPVPFGATATLITDKQ
                     NEVSSIVGEEGQLYISGMPEEGRVLIKWGNDASQQCVAPYKLSLELKQGGIIPVSANC
                     Q"
BASE COUNT         3514 a         3937 c         3610 g         3605 t
ORIGIN      
        1 cacgttactt ccgcttgttc agccagttca cgatcatact ggcaagaatg ccagcaatga
       61 ccggagccgc taaatcatgc cagaaggcca tgcccagttc tgcgagcgtc atatagccgc
      121 ctgtgttgta atgacaacgt ttcgcggcta ttcttgagtg gtctaggttc aagattagcc
      181 cccgtggtgt tgtcaggtgc atacctgcaa cgtgcggggg ttttctctct ccagcaacca
      241 atgccaccag ggattaagcc cccgcaacat tgcgcctcac cggaaccctt ccggcttgcc
      301 gctaatttta cgattgcttt ttactgtcag cactcggcaa tcttcctcat tgcgtgatgc
      361 cttccagctt aatttcttaa tttgcaatag ttgcagttca tatccgggct gactccagac
      421 tggtagtgaa atgcgcaatt ttgtagaatt gcgcctcctc gcgacctccc cagtgaatct
      481 gttgcctcat tttttacata ctgcgtattc gacttctcca cctgttgcgc aagagaaact
      541 gggtttattc atttttgcga ggccgacttc tttctggaca ggactttatg cagcacaaca
      601 cactaccgaa acacgatcag aaattgccgt ttacacgcta cgacttcggc tgggttttat
      661 tatgcatagg catggcgatt ggtgccggaa ccgtgctgat gccagtacaa attggcttga
      721 agggaatttg ggtatttatt accgcagcga tcattgctta tcctgccacc tgggtagtgc
      781 aggacattta tttaaaaacc ctttctgaaa gcgattcctg taatgactac accgatatta
      841 tcagtcatta cctggggaag aactggggaa ttttcctcgg ggttatctac tttttgatga
      901 ttatccacgg gatttttatc tactctctct ccgtggtttt cgacagcgcc tcgtacctga
      961 aaaccttcgg tttaaccgat gccgatcttt cacaatctct actttataaa gtcgctattt
     1021 tcgccgtact ggtagcgatt gcgtctggtg gtgaacgatt actgtttaag atttctgggc
     1081 caatggtggt ggtcaaagta gggattattg tcgtgttcgg ttttgcgatg atcccgcact
     1141 ggaatttcgc caatataacc gccttcccgc aagcctccgt ctttttccgc gatgtcctgc
     1201 ttaccattcc attttgcttc ttttctgcag tatttattca ggtacttaac ccaatgaata
     1261 ttgcctatcg taaacgggaa gcggataaag tactggcaac ccggctcgcg ctgcgtaccc
     1321 accgaattag ttatatcacg ctcatcgcgg tgatactgtt ttttgccttt tcgtttacct
     1381 tctcaattag ccacgaagaa gccgtttctg cctttgaaca aaatatctcg gcactggcac
     1441 tggccgcgca ggtgatccct gggcatatca ttcatatcac ctctacggtg ctgaatatct
     1501 ttgccgtact gaccgcattc tttggcattt atctcggttt ccacgaggcc attaaaggca
     1561 ttattctcaa tctgttaagc cgaattattg ataccaagaa aattaactca cgcatgctga
     1621 ctctggcgat ctgcgctttt atcgtcatta cgttgacgat ttgggtttcg tttcgtgtat
     1681 cggtgctggt gttctttcag ttgggaagcc cgttatatgg cattgtgtcg tgcctcattc
     1741 cgtttttcct gatctataaa gtcgcacaac tggaaaaact tcgcggattt aaagcctggc
     1801 tgattctgct ttacggcatt ttgctatgct tgtcgccact gttgaagctg attgagtaaa
     1861 ccggagcgca tggccccggt tttgtgagtt aacgctgcgg attttcatcc tgatcaacag
     1921 caaaacaagc taccagttga ccgccgtagt cttttagctg cggctgcaac tgggtgcagg
     1981 ggccgaagcg ccgacgacag cgggcgttaa acgcgcagcc cggcggtgga ttcagcgggc
     2041 ttggcagttc accggtgagt ttgatgcgct cgcggcgatc gtccgggttc aggcgtggtg
     2101 tagcggaaag tagcgtctga gtgtacggat gacgcgggtt attgaagatt tggtctttcg
     2161 ttcccttctc cacgcagcgg cccaggtaca tcaccatcac ttcatcagca atgtgctcca
     2221 ccaccgacag gtcgtgggag atgaagacat aagaaagccc cagttcctgc tgcaaatcca
     2281 tcatcaggtt gagcacctgt gcacgcaccg agacgtccag cgcggagacc ggttcatcgg
     2341 caatcaccac gtccgggtcg agcatcagac cacgggcaat agcgatacgc tgacgttgtc
     2401 cgccggagaa catatgcgga tagcggtcat agtgctcggt tttcaggccg actttcgcca
     2461 tcatcgacaa ggctttttcc cgacgctgtt ctttgcttaa gctggtatta atcagcagcg
     2521 gctcctcaag aatttgcccg acttttttac gcggattcag cgaaccgtaa gggttctgga
     2581 agacaatctg gattttctgc cgacgcagct tctgcgcctg cggatcgtgc ttaagcagat
     2641 cctgcccctg gtaatacagc tcgccaccgg tgggcgtttc aatcatcgtc agcaaccgac
     2701 cgagggtcga tttaccgcag ccagattcgc ccactaccgc cagcgttttg ccacgttcaa
     2761 ggttaaacga aacgccatcc agcgctttaa ccagacgttc cggcgcgaac atgcctttct
     2821 tcaccggata atgttttttc aggtcgatag cctgcaacag cggttgttgc gaggtggcct
     2881 cttgcgtact catagtgtcg gcctcccggc atcatcaagt gggtaatggc atttggactg
     2941 acgcccatca gcgagcatat tcagcgccgg ttcttcagcg cgacatctgt cagtggcata
     3001 ggggcagcgc gggttaagca ggcagccgtt cgggcggtcg tacttgccgg gaacgacacc
     3061 tggcaacgat gccagacgtt ctttgtcctg agcaaattcc ggcagcgcac gcagcaatgc
     3121 ctgtgtatac gggtgacgcg gcgcatggaa gatggcgtgc gcatcgcccg tttccaccac
     3181 ctgaccggca tacatcacga tgattttatg tgccgcttcc gccaccagcg ccaggtcatg
     3241 ggtaattaac accagcgcca tgttctcttt ctgctgtagc tccagcagta gttcgatgat
     3301 ttgcgcctga atggtcacgt ccagcgcagt ggtcggttca tcggcaatca gcagttttgg
     3361 ccgacaggca atcgccatgg cgatcatcac gcgctggctc atgccgccgg aaagctgatg
     3421 cgggtaaaca tccagacgcg atgccggatc gggaataccg acctgattca gcaggtcaat
     3481 cgcccgctga cggcgagtgc tcttattacc gccctgatgc accttaatcg cttccataat
     3541 ctggaaacca acggtgtagc acgggttaag gctggtcatc gggtcctgga agatcatcgc
     3601 cacttcggca cccaccagat tgcggcgctc tttttctgag atacgctgca aatcctgacc
     3661 gttaaactcc agcttttccg ccatcacgcg gcccggataa tcaatcagcc ccataatcgc
     3721 cagtgaactg accgacttac cagagccgga ctcacccaca atcccgacca cttcgccctg
     3781 tttcacgctg tagctgatgc ggtctacggc gcggaacggt gcgctttcgt cgccgaaatg
     3841 caccgataat ttatctacat ttaataacgc catctcgaac ctcttactgc ttcagtttgg
     3901 gatcgagcgc gtcacgcaga ccgtcaccca tcaggttaaa tgccagcacc gtcagcagga
     3961 tcgccagacc agggaaggtc acgacccacc aggcgctttg cgcgaactgc aacacgtcag
     4021 agagcatggt gccccactct ggtgttggcg gctgcgcccc catgccgagg aaaccaagag
     4081 cagccatatc gagaatggcg ttagagaaac cgagcgacgc ctgaacaatc agaggcgcaa
     4141 ggcagttcgg gaagatgtta ataaacatct gacgcatcgc cccggcacct gccacgcgag
     4201 acgcggtaac gtaatcgcgg ttaacttcca ccagcacggc ggcgcgggtt aagcgcacat
     4261 agtgcggcaa ggcaacaaag gtcagcgcca gcgcggcgtt accaatcgac gggccgaaaa
     4321 tcgccaccag caccagtgcc aacagcagac ttggcagcgc cagcatgata tcgaccacgc
     4381 gcatgatgat gttatcgacc aggccgccaa agtaaccggc gatcagtccg agaataacgc
     4441 ccataatcag cgataacacc accaccagac agccaaccag cagcgataaa cgcgcgccgt
     4501 acatcagccg cgacagcaca tcacggccta cgtcatcggt gcccagcaag tgcgccatgc
     4561 tgccgccttc ctgccaggcg ggcggggcaa gcagagcatc gcggaattgt tccgccgggt
     4621 tatagggagc aatccagttg gcaaagatcg cgataaacag cacgatgacg acgtaaacca
     4681 gcccgacgac cgcgcctttg ttgcgtttaa aatagtgcca gaactcctgt aacggggtca
     4741 tcggcaccgg tgcgctaatc actttatttt cagtaacctg tgacatgatg tccccttact
     4801 tcttatgacg aatacgcggg ttcaccacgc cgtacagcag atcgaccagc agattgacga
     4861 ggataatcat cgtcgccacc agcaatacgc cgccctgcac taccggatag tcgcggcgtt
     4921 gcagtgcgtc aatcaaccag cgccccagac cgggccacga gaagatggtt tcggtcagaa
     4981 tcgcccccgc cagcaatgtt cccacctgga ggccgataac ggtcactacc ggcagcatcg
     5041 cgttacgcag cgcatggacg ataatcaccc gcatgcgggt caaccctttg gcgcgcgcgg
     5101 tgcggatgta atcttcaccc agcacttcca gcatcgagga gcgtgtcata cgcacaatga
     5161 ccgccagcgg aatagtaccc agcacaatgg cgggcaagat catatgggcg acggcatcga
     5221 taaagttacc gtcttcaccc cagatggcgg tgtcgatcag cataaaaccg gttaacggat
     5281 tggaatcatc gaggaacacc atatcgctca cgcgaccgga gacgggtgtc aggttccagt
     5341 gcaccgaaac cagcatgatc agcatcatgc cccaccagaa gataggcatt gaataacctg
     5401 tcagcgccag gccaaccgct gtgtgatcga aaatggaacc gcgtttaacc gcagccagca
     5461 caccaaccgg aataccgact gccgtagcaa aaatcatcgc gcagacgcca agttccagcg
     5521 tggcctgaaa gcgcggcacg aactcttccc ataccgggat gcggcttttc attgaaatgc
     5581 ctagatcgcc atgcataacg ccccaaatgt aatggagata ctgctgccac atcggtttat
     5641 ctaagccgag ttcagccagc aactgcgcgt gacgctctgg ggagatccca cgttcgcccg
     5701 ccatgatcat caccggatcg cccgggatca tgtggacaaa ggcaaatgtg agaagggtaa
     5761 taccgataaa cgtggggatg acgagtccca aacgtcggag aataaactgc aacataaccc
     5821 ggattctctg tagtgacccg cagcctggaa ctgcgtgtct gtattgctca caaatcaatt
     5881 cccgcccagg tcgggcaggg aatgaagtat tgctgccgga tggcgctgca cgcagaggca
     5941 cagcaccacc gggtatggct tttaattatt cgatagagac gttttcgaag tgatgtttgc
     6001 ctaatggatc aaccacatag cctttaactt ctttgcgtac cggttcaaac acggtggagt
     6061 gagcgatgat cagtgccgga gcctgatcgt gcatcaccac ctgcgcttgc ttgtacagtt
     6121 caacgcgttt gttatggtcg tcggtagcac gcgccggttg aatcagatct tcaaacggtt
     6181 tgtagcacca ttttgagtag ttggagcctt gttcagaggc ggcgcagctg aacagagtgg
     6241 cgaagaagtt atccggatcc ccgttatcgc cagtccagcc catcattacc gtctggtgct
     6301 cgccatcttt cgcacgcttg aggtactcac cccattcgta ggtgacaatt ttggcctgca
     6361 cgccgacttt cgcccagtct gcctgaatca tctccgccat gcggcgagcg ttcgggttat
     6421 acggacgttg taccggcatc gcccacaggt cgatggagaa acctttttcc agacccgctt
     6481 ctttcagcaa ggctttcgct ttttcaggat cgtaggtgta gtcctgaacg tcgtcgttat
     6541 agccccacat ggttggcggg atcaggtttt tcgctgatac acccgcgccc tgataaaccg
     6601 ctttgatgat cgcgtctttg ttcaccgcgt aggtcagagc ctggcgaact ttcacgtcat
     6661 cgagtggttt tttctgcacg ttatacgaga gataaccgac gttcagcccc ggcatttcca
     6721 tcaggttgat agatttatcc tgcttcatgc gggcgatatc tgccgggttc gggtacggca
     6781 tcacctggca ttcatttttc tgcaatttag cgtaacgcac ggaagcgtca ggggtaatag
     6841 agaaaaccag cgtatcgatc tgcggtttgg tgccccagta gccatcaaac gctttgtagc
     6901 ggatacggga atctttttga tactgctgta actggaacgg accggttccg attgggttga
     6961 ggtccagttt ttccggtgta ccggctttca tcatcgcatc agcatattct tttgacagaa
     7021 tagaggcgaa gtccattgcc aggtcagcga ggaacggcgc ttccgggcga gtcagcacaa
     7081 actgaacggt gttatcgtcc acctttttca cttcgctgat cagctctggc aagcccatgc
     7141 cttcgaagta ttcgtagctg ccgccagaaa ctttatggta cgggttttgc gcgtttttct
     7201 gacgatcgaa cgagaacacc acgtcatcgg cgttcagttc gcgcgtcggt ttgaattctt
     7261 tattgtcgtg ccacttcaca cctttacgca gatggaaggt gtaggtttta ccgtcttcgc
     7321 tgacttccca cttttcagcg aggcccggga tcacttcagt ggtgccgatt ttaaattcaa
     7381 ccagacggtt ataaagcggg acggaagagg cgtcataggt ggtgccggag gtaaacagct
     7441 gcgggttaaa cccttccgga gatccttctg agcaataaac cagagtttta gcctgaacac
     7501 ttgctgcgac ggtcatagcc accaggctga gaccaagctt cagcatccct gactttttca
     7561 aggaaatacg cattattctg ctccaattgt gatgtttgtt gttttaaccc tttgcagtgg
     7621 gttgtcgctg cctgaccttt tttgtttttt acccggtcgg gtcaacgtta tgaggtgggg
     7681 atgccgtact aattaacatc agtgtaacag tgcggatcct ccataaaatg cccctcgtga
     7741 cctacaatct gtcaacagaa tgtgaaaacg tcaatacagc caaccgggat ttacaccaac
     7801 ggtgagaatc cacaaacaaa gattaaaaaa acttcaaaca gctatttgca gcagagaaat
     7861 ttgtgctact ccaacatgac cagaacaatc agcttaatat ttagcaacat ttggtgatta
     7921 agttttatgc atttaatgaa aaaaatctga ggaaaaggtg gatatctgag cgattaatac
     7981 catgaacgct aaaacgcaca gcggaaaatg ccagcgccag ccataagtaa cgcaaaaaaa
     8041 gatttaagca aatataaaaa aagacaatgg tttctgtcac aaatccgtta atggatagtg
     8101 agatatgggg cgcaaatttg gcagggaaat gtgtggtgtg ctgccgttta ggtcggataa
     8161 gacgggtacg ctgcatccga caatgagtgc gctgccgttg cctgatgcga cgctgacgcg
     8221 tcttatcagg cctacgtcga gtcgaacctc cctgcccccg gaggttctca tcctcttcag
     8281 gtcgcagaaa gcaaaaaacc agcccgtagg ctggtttttc taaattggtc ggtgatagag
     8341 gattcgaacc tccgacccct tcgtcccgaa cgaagtgcgc taccaggctg cgccaatcac
     8401 cgaatgcggg gcgcatctta ctgcgcagat acgccctcgt caatccctta atagcaaaat
     8461 gccttttgat cggcgagaaa gtcagcaggc ctcttagtta gccactgcct cttttgtctg
     8521 cgggatgtga caccagttgt tgttttcgtt aattccacca tccggtgaag tatagccaag
     8581 acaacccatg atggtgtcgt acagctctac gtgacggcgt ggcactttca tgtcggctgc
     8641 ttttttcagc tgcgcaaacg cctgcgcatt gaccggattt tccagatatt tatctgacat
     8701 ccagaccatc atcggtacgc ggaactgctc cggcggtgcc agttcacgcg gcgtgccgtg
     8761 caggtgctcg cgttcattaa ttgactcgcc gtggtcagct gcgtagaaca caatcgcttt
     8821 cttatcgcga acctgatcaa tcacactgga gataaagtga tccacatagg tcaccgagtt
     8881 gtcataggag ttgatcatct gcgctttggt acatccgcta tcaacaccaa tacattccgg
     8941 cttccactgc gcaaagctgc gcgggtaacg ctgggtgtag ttaaagtgcg aacctttggt
     9001 atgcagaatg attagatgct taccatccgg gttgcgcccc agcgattgct gcatttcgtc
     9061 taccagcaac atatcatcta ccggcttgcc acggttacgt ggctccgcac caatctgttc
     9121 acggtaagca atattgtccg ccatcgtatt gctgtagaac cacatttcgc tctgcatagc
     9181 gtagaggtca gaactgaatc ctaactgctt cagtaccgcg aaaatgttct gttcttttaa
     9241 tgtgcgctgc ggattatctt ccgcgccccc ctgacgtaca aacatgcaac gcagagagag
     9301 tttggttgcg gtatcacagg agtaaccacg gaacgccgcc agatttttct cctgggccag
     9361 cttcggcgtg gtattacgct catagccgaa aatgcccata tggtcccaac gcgtggtttc
     9421 accgatgata aacacgacat aagtatcatt aacattttgc ggtgcctgat aagtgaattt
     9481 cttcgccggg ttaagcaatg aattattatc ggaagattca tcgacccgcg cccaggcgta
     9541 cagccccaac gcagaaagcc agtttgatgg cagataagag ttcgctacga caccgccata
     9601 actcggcaaa tcaacgccgg tcgccctctc cactttcttc tgctggatat ccagcaaacg
     9661 aatcggtgcc caaaccataa ttcccgccag tacgacgacc gccaggctgc gaatacgctg
     9721 ccctggggtt cgcagttgtc ggagcaaggt gtagcgacag cggttattcc agataaggat
     9781 aagaggcaat gcgctaacgg cgattaacca gagaataaag ttcagaccaa caacttcttt
     9841 tgacaggtcg atatcggtgg tcatgacgga agcgatgatg ccataaccaa tgaccacatt
     9901 aaggaaggtc atgtaatagc tggcacctgc ggaaaagagc acaaccagcg atgccagaat
     9961 acgccaactg cggcggccaa acagcgaaag aagacgtagt aaaaagaagg tcaccagtac
    10021 ggtggcggcc agttcaacaa cagcagaaat gcctttccag acggtaaaat cgtgcgcata
    10081 gctgccgaag cggcggtaaa aaaccgcgcc attcataaaa aggccaatat agattgcaag
    10141 caaaaagctc agcttctgct gtgtaatcga tttgatgtat ctcatgcaaa caaaccctgg
    10201 acaaacaggt gataacaccc tgcttagcgg cttttcaggc ccagcagggg aaaagtgcgg
    10261 aggtagtcag ataagtaaaa tctacacgcg cgaataagta gaccacagca aagggaaaaa
    10321 gtgtggggaa agagtgtgca tgaagcacaa atttacaaaa gtgcaagtcc ggaatagacc
    10381 attttaacgg attaacgact attcagagat aggttgggcc aggatgctgg cccagtaact
    10441 tactcatagg tgatggaaaa attgacaagg gcattcgctg tgccaccagt ggcctgagtg
    10501 ttgacgacca cataactcgc ctggtagtga agcgacgtgg tttcgccatc aatcgcccat
    10561 tcaatcggcg ttccgctgga gcctttggga acttgctgcg ttcctttatc gtcctctatc
    10621 ttcaacgcaa cacccgttgc gccaccagcc tcaagggcaa acatggaatc atcatcgctg
    10681 tccgactgac tgtccatctc aacggtcgct ttggatatcc catcgcactc tttgagttca
    10741 atggtgaaat ctttcttgct caacaccgta ccgactgccg caggtttttg cagcgtcatt
    10801 gttcccatat cgacttcgag atcgcttttt tccatctcgc aagctttagt ggtgatagtg
    10861 atggagaaat gaatatcccc tgccgggcca agatccgtcg ccgcattcac cccgcaggca
    10921 aacagactca aactggcgat gccgctatgc agcaatagat tgaatttcat ggcatcactc
    10981 catttcaaga tatatggtcg ttacgccttc aaacgtcccg cctttaggcg ctttacccgt
    11041 cgtgctaatc ggtaaagcca gcagtcgaat cgcggcattg cggtgagttt tagaatccag
    11101 gctgctcaaa tttaataacg tggtgctgct tgcatcattc ggtaccaaaa ttttgccgct
    11161 ggaatctttc accagtacgc caatatcggg gttgcccata tccaccgctc ggggatcgtc
    11221 agcattggtt gccccctcaa ggcgcaacga gactttgata ccatccgaga tattgttgca
    11281 atcaaagccg aggttaattt cttgctctgt cgcaccttca ggcatttgcc cttgtctgtt
    11341 tttaaacgct gaggagggga attcaccaaa tggcacatcc agcaccgtgc cgggctttat
    11401 ttcgcaatcc tgagttatgg tgacgcggcc ctgaatagat acggaagata tgggtattgt
    11461 tccgtaatct ttatttgttt tggaggcata cagttcggca attaatgtca gagggatgac
    11521 ggaatcgccg ataaagggtt tagtaatagc aaaggaaagt agacctgcgc cgcccatata
    11581 gggcgtagta atgactttgt tgcatccggt tgtttgcagc gcctgctggt ttttgaaagg
    11641 aacaaatcca tacacactgg tactaatgcg aattttcgtc gcaacctcca gttcctccgt
    11701 caaagtataa tatttcatac tgtcaaccga acggccagaa gggggaagca tactcacccc
    11761 ttgtactaga gtatcttttt cattcttata agaatcagga cattcacaaa ttagcgatac
    11821 gttttgtcca ttagaccagt taaggacatc cggtaaaacc gttcctgcga cgttttttgc
    11881 cgcatcaggt ataccaacat ccacatcgac attatagtga taagtcgtgc catccgcggg
    11941 ggtacatggc ccccacgcat ctccggcaaa tgccttgcca gaaaatccga atacgcatcc
    12001 taataaggat aatgctattg ccgctttcat ttcgctctcc ttgcttactg gcaattggcc
    12061 gaaacaggaa taattccgcc ctgttttaat tccagggata atttataagg cgccacgcat
    12121 tgctgcgacg cgtcattacc ccatttaatc aatacccgtc cttcctctgg cattccgcta
    12181 atatagagct gtccttcttc accaacgata ctgctcacct cgttttgttt atcggtgatt
    12241 agcgttgccg ttgctccaaa gggcaccggg ctgcccgcgg aacgaagcag cgtcattaat
    12301 acgcggtaac caacgcgggt atcaaaacgc gctctgacaa tcgctccacg cgttggcacc
    12361 actttctgga acgcattttc aacgtcaaca tcgtcaccca acgaatcgct acgcagtgag
    12421 atttcgtttt cacgatacgg catggcgtaa ggcacgatcg cgttacccat ccagtcaacc
    12481 tgaacgccgc cgttattcac cacgcgggca ttgcgcgcac cgggtacgga gatgagcgtc
    12541 atggtttcgc ctagcggttg ggaaagcgtc acgccttcgc tgtgaacgat cacgccgccg
    12601 cgaacgctgt agttgagctg ccggtaatct ttcccgtagc tatagcctac gttgacatta
    12661 ccggacccac cctgatagcc aacgttggca ttaccgctat tacccacgcc gttgctggcg
    12721 tagccttcct gaacactgta gctcaggttg cgatcctcaa gcagcgtgcc gctgacccca
    12781 acctgttgat tggtgcgacc atcctggtcg gtcgtgacgc gatagtcact ccaggcccgg
    12841 cctagtggaa cggaaatatt gaaagaccac aagcgatcgc tttcatccca ttcagggctt
    12901 ttattccagc tatatgcaat actgtaactg accttgccaa tacgtccgtt gtaacccgcc
    12961 gataccgtgt tctgtttacc tgcgtcgttc cagtaatcct gctgcgttaa atttaaataa
    13021 acagagccat aggcccctaa ttgctgcgtc aggttaccct gtatttcgct gcgcttgtga
    13081 taacggttat agtcgctgtc agcgtcactg cgcacatcgg tggcttcctg gaaggtatag
    13141 aaaccgctgg tagagtaccg atagcccgca aggcggaaat cggtgccgct ttcgaagctc
    13201 ttggaatata agaaacgata agattgtccc tggctatcgc gatcgttatt cagttcgctt
    13261 ttagcctgcg tcacatcaat ggaaatcgcc ccgatataac caaagttttt ccctatccct
    13321 aatgcaaatg cattgtaatt attagagatt aatacgccgc cgtaggccgt catcccccac
    13381 ggtaaaccgt agatcagatc aagctgcccg aaacgcggcg aggcgctgtc ataattcccg
    13441 gcctgatatt cacctgcggc aaagctatat ttgagatggc cttcacgctg gaaaattggc
    13501 accgatgaga atggctgaat aaagcgttgt tcactgccat ccgcttcttt aataatgacc
    13561 gtgagatcac cgctgtttgc agtggggtaa agatcgttta tttcaaaggc accaggctgc
    13621 acataggtgc gataaatcac gtagccgttt tgttcaatac tgacttcggc gttgcttttg
    13681 gcgatccctc gaacgatggg tgcaaagcct cgctggctgt cgggcagcat ctcatcatcg
    13741 gaggctaaca tggcaccgcg catctgaacg ctgtcaaaaa tatccccggc ggttgccgta
    13801 tctcccatcg ttaattgcgc tttgaaaggg acgatggccc tacttaaaga cgtaccgata
    13861 ttatcccact gcgctttgcc gccggaatgg ctccaggtac tgtaattacg cagtcgccac
    13921 gcgccaaggt tcagcccgct gcgtaagttg agatagtagc tgtcgctctt tagcgtatcg
    13981 tcgccatcat catgatgaac ggtgccatta tcatcgacat aactgctgga agaccccgaa
    14041 gagtcatatt cactgttgct cccgctaaaa ctgtaatcca gcaataatgc cgggatacct
    14101 tcgtcccata aagattccgg caccgtgccg cgcgccacct ggtgcatcgc cgcctgaggg
    14161 aaacttaata ccagcttttg ttcactgaag tcgaaatggc ttgaggcctg gggaatgatt
    14221 tcgtcaaacg gtacgcaggc ttcggcaggc acctgattca gggtcggaaa gctttcaacg
    14281 cgcacgccca tctctgccag ttgcgtccgg ctaaggcaag gcacaaggga ttcccccatc
    14341 gctttgcgct gtgcttctgt ggcctcttta aattccagtg tgcgggtttc cattctctgt
    14401 tcattgacgt aaacagagac acgatagatg ccgggtaact gctgctcctg gcctttttcg
    14461 aacatcgtta aatcgacatg ctggcccaca ccctgaacgt cctccaggaa tttggggtta
    14521 aactttccgg catgtgcaga aaacgtgctg gcaacagcaa ccgccaccac agagatagaa
    14581 aaggaagaaa aagtacgtga aacggtcttc cgtgacattg agctatttct cctgaaaaga
    14641 tattaatcaa tcaacatttt tagtga
//
LOCUS       DAZUCE010000122        14599 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZUCE010000122 DAZUCE010000000
VERSION     DAZUCE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.122"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(16..168)
                     /gene="hokE"
                     /locus_tag="ACHY03_003117"
     CDS             complement(16..168)
                     /gene="hokE"
                     /locus_tag="ACHY03_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFS3906316.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            137..405
                     /locus_tag="ACHY03_003118"
                     /pseudo
     CDS             137..405
                     /locus_tag="ACHY03_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            609..1727
                     /locus_tag="ACHY03_003119"
     CDS             609..1727
                     /locus_tag="ACHY03_003119"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFS3906317.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1793..2041
                     /locus_tag="ACHY03_003120"
     CDS             1793..2041
                     /locus_tag="ACHY03_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFS3906318.1"
                     /translation="MKHPLETLTTAAGILLMTFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTFLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2106..2474
                     /locus_tag="ACHY03_003121"
     CDS             2106..2474
                     /locus_tag="ACHY03_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS3906319.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2568..3221
                     /gene="nfsB"
                     /locus_tag="ACHY03_003122"
     CDS             2568..3221
                     /gene="nfsB"
                     /locus_tag="ACHY03_003122"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFS3906320.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3329..4576
                     /gene="ybdG"
                     /locus_tag="ACHY03_003123"
     CDS             3329..4576
                     /gene="ybdG"
                     /locus_tag="ACHY03_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFS3906321.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4644..6020)
                     /gene="pheP"
                     /locus_tag="ACHY03_003124"
     CDS             complement(4644..6020)
                     /gene="pheP"
                     /locus_tag="ACHY03_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFS3906322.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6122..9265)
                     /gene="cusA"
                     /locus_tag="ACHY03_003125"
     CDS             complement(6122..9265)
                     /gene="cusA"
                     /locus_tag="ACHY03_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFS3906323.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVKIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVIWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9277..10500)
                     /gene="cusB"
                     /locus_tag="ACHY03_003126"
     CDS             complement(9277..10500)
                     /gene="cusB"
                     /locus_tag="ACHY03_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFS3906324.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFDRAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10516..10848)
                     /gene="cusF"
                     /locus_tag="ACHY03_003127"
     CDS             complement(10516..10848)
                     /gene="cusF"
                     /locus_tag="ACHY03_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFS3906325.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10872..12254)
                     /gene="cusC"
                     /locus_tag="ACHY03_003128"
     CDS             complement(10872..12254)
                     /gene="cusC"
                     /locus_tag="ACHY03_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFS3906326.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPHAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12411..13094
                     /gene="cusR"
                     /locus_tag="ACHY03_003129"
     CDS             12411..13094
                     /gene="cusR"
                     /locus_tag="ACHY03_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFS3906327.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13084..14532
                     /gene="cusS"
                     /locus_tag="ACHY03_003130"
     CDS             13084..14532
                     /gene="cusS"
                     /locus_tag="ACHY03_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFS3906328.1"
                     /translation="MVNKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEIGKVFDFFEALAEDHGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIVLPERG"
BASE COUNT         3522 a         3959 c         3604 g         3514 t
ORIGIN      
        1 ctccccgcac aacggctact tcttcggttc gtaagcgaga acagccctga actcaatatt
       61 acgttccttc accgtaaact cacacagcga gtcgccgacc agaagcgtaa atcccagcac
      121 cgtcaaacac agcactatga ctgccacaag ggcatatttc gtcagcatgt ttatattgcc
      181 tccatgcgaa gaagggacta ccatccaaat tgttcaggtt tggagtggca gccccagggt
      241 tgatagaaat atctcctggg gcttcatctt tccagacctc agagtaaacc tgaaaccgga
      301 aagcttcagg cacccgccgt tatcttaccc ttcctcaaca aaatggctgt ttatttatac
      361 agtaaatcct gtttcggact tctcactcct catttgatca actgaagctg agcccgtttt
      421 ttctgcatgc ctcaatcgtc aaaattcttc gctactagcc agccgtgcca taacttgtca
      481 cattgcaaaa gtagccaaat tgcttcaggc accatcacca aaaaaaagaa acattccaga
      541 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg ccaacataag
      601 agaaacctat gccattaccc gattttcatg tttctgaacc ttttaccctc ggtattgaac
      661 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      721 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      781 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg caattttcag
      841 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      901 gcacgcaccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      961 gaacgctgga aaactttggc tatctcattc aacaggcgac cgtttttggt cagcatgttc
     1021 atgttggctg cgccagtggc gatgacgcca tttatttgct gcacggcctg tcacggtttg
     1081 tgccgcactt catcgccctt tccgccgcgt cgccatatat gcaaggaacg gatacgcgtt
     1141 ttgcctcctc acgaccgaat attttttccg cctttcccga taatggcccg atgccgtggg
     1201 tcagtaactg gcaacaattt gaagccctgt ttcgctgttt gagttacacc acgatgatcg
     1261 acagcattaa agatctgcac tgggatattc gccccagtcc tcattttggc acggtggagg
     1321 ttcgggtgat ggataccccg ttaaccctta gccacgcggt aaatatggcg ggattaattc
     1381 aggctaccgc ccactggtta ctgacagaac gcccgttcaa acatcaggag aaagattacc
     1441 tgctgtataa attcaaccgt ttccaagcct gtcgctatgg gctggaaggc gtcattaccg
     1501 atccgcacac tggcgatcgt cgaccgctaa cggaagacac cttgcgattg ctggaaaaaa
     1561 tcgccccttc cgcacataaa attggtgcat cgagcgcgat tgaggccctg catcgccagg
     1621 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggtggctcgc
     1681 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagta gggaattgcc
     1741 ttacagaaga atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca
     1801 ccctttagaa accttgacta ccgcagcagg cattttgctg atgactttcc tctcttgcct
     1861 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1921 tctgatggat cttagtcagc tttacacttt tttgttttgt ctgtggtttt tagtgctggg
     1981 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     2041 aacgtcgatt atcgccgtac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     2101 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctggccctt gagttaccct
     2161 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2221 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag tccgatccac
     2281 aaaaatccct gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata
     2341 aaaagcactg gatttcggtg tatcccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2401 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2461 tgcgtccagg ctaaagcgga aatctatagc gcatttttct cgcttaccat ttctcgttga
     2521 accttgtaat ctgctggcac gcaaaattac tttcacatgg agtctttatg gatatcattt
     2581 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2641 aacaggccga gcagatcaaa actctcctgc aatacagccc atccagcacc aactcccagc
     2701 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2761 ccggtaatta tgtgttcaac gaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2821 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgctg
     2881 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2941 ccgatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     3001 tcaacgtcgg taatttcctg ctcggcgtgg cggctctggg tctggacgcg gtacccatcg
     3061 aaggttttga cgccgccatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     3121 gtctggtggt agttccggtg ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3181 aatctcgtct gccgcaaaac attaccttaa ccgaagtgta attctccctt gccgggcatc
     3241 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3301 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3361 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3421 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3481 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3541 tttagctttt accctacagg ggattatcgt caatattcag gcggtattct ggctgcaaaa
     3601 aggcaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3661 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccggc
     3721 agcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcgattct
     3781 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc tgatcagcgg
     3841 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3901 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggagatgcc
     3961 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     4021 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     4081 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     4141 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4201 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4261 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4321 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4381 actggctcca ggtgataacg gtttaccgct tgagatctat gcgtttacca acaccgtggt
     4441 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4501 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4561 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4621 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4681 aatacaatcc acaccggcag cagaatcgct gacaagcgca tatcatccat cgtgcacatc
     4741 agcagcaaaa tcatgccgag gaaggcaatg caaagatagt tgccgaacgg ataaagcagc
     4801 gccttaaact gtgtttcacg tccctgacgt cgcatcgccg cgcgaaaacg cagatgcgca
     4861 aggcagatca taatccagtt caacagcagc gttgctacca ccagcgccat cagcagacca
     4921 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4981 agcatcagcg agttaatcgg cacgccgcga cggctgacgc gagtcaaaaa cttcggcgca
     5041 ttaccctgta cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     5101 actgacagcg atgctaccag aatgacgaag ttcagcgcag aagctaccac gttgctgtcg
     5161 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5221 agcgccagta aaaccaccag tgaaccgatg taaaacagca ggatgcgata caccacctga
     5281 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggcagt aatcccaatc
     5341 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5401 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5461 tgaccagaaa acagcagcca caggccaaag ccgatcatac cgatgattgc cagcacttta
     5521 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5581 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5641 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa catcacccag
     5701 taattccagc cagagaggaa gcccgcgaac ggtccccagt atttataggc aaagtgggca
     5761 aatgaaccgg ataccggctc ctcaaccacc atttcgccaa gctggcgcat aatcaggaaa
     5821 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5881 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5941 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     6001 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     6061 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacggg gtatcctgct
     6121 tttatttccg tacccgatgt cggtgcagcc acatcagctt atacgccgcc gggataataa
     6181 acagcgacag caaaggcgcg gtgatcatgc cgccaatcat cggcgcggca atccggctca
     6241 tcacctctga ccctgctcct gttccccaca gaatcggcaa cagaccagcg ataatcaccg
     6301 ccaccgtcat cgctttcggg cgcacgcgca ggaccgcgcc gtgatacaac gcctcatcca
     6361 gcttctgctc gctgaatgtt tgcggattat tcaacgacgg ctcggcctct atggcgtgac
     6421 gtaaatacat cagcatcacc acgccaaatt cggcggcgac cccggcaagg gcaataaaac
     6481 cggtgcccgt cgccacagaa agatgaaagc ccatccacca cagcaaccag atgccgccca
     6541 ccagcgcaaa cggtacgctg ctgataatca gcaacgcttc gcccacccga cggaacgcca
     6601 gatacaacag cacgaagata atcatcaatg tcatcggcac catgagttta agcttatggt
     6661 tggcgcgctc cagtagctcg aactgcccgg agaatgccac gctggtgccc ggttttaact
     6721 gcactttttc agctatcgct ttttgcaaat cgtgaaccac cgacaccatg tcacgatcgc
     6781 gggcatcgat ataaatccag ctcgtcgggc gcgcattctc ggttttcagc atcgacggtc
     6841 cggtagagac tttaacgtcg gctacgtctg ccagggtgat ttgctgcttc atcggcgtca
     6901 ggatcggcaa ctggcgcagt gcctgcggac tatcgcgcca gctttgcgga taacgcagat
     6961 taatgggata acgggcaatc ccttccaccg tttcgccaac catcgccccg cccaccgcag
     7021 aagtcacaaa caactgcaca tccgccaccg tcataccgta acgcgcggct ttttcacggt
     7081 taatctcaac gttgatatag cgcccacctt ccagacgctc ggcaagcgca gaagccacgc
     7141 ctggcaccgt tcgtgccacc tcttcaattt gctccgccat cgcgtcgata tccgccagca
     7201 cagtgccgga aacttttatg ccgatagggc ttttaatgcc ggttgagagc atatcgatac
     7261 ggttacgaat tggcggcacc cacagattcg ccagccccgg cagacgcacg gtgttatcca
     7321 gttcctcaat gattttgtcc atcgtcatgc ctggccgcca ctgatcctgt ggcttaagct
     7381 ggatggtcgt ttctaccatc tccagcggag cagaatcggt ggcggtttcc gctttcccgg
     7441 ttttgccaaa tacccgcgcc acttcaggta cgctcataat tagcttgtcg gttttttgca
     7501 gcatactcgc cgcctctgct gcggaaatcc ccggcaacgt cgatggcata tacaacaagt
     7561 cgccttcatt gatctgtggt aaaaattccc cgccaacttt attgagcggc cagataaccg
     7621 tcagcaccga aagcgccgcc accagcagcg tggttttcgg ccagtgcagt actttcagca
     7681 acagcggatg ataaacacga atcaaaaagc gattgagcgg gttactgctt tctggcggaa
     7741 ttttgccacg gatccagtaa cccatcagga tagggatcac tacgatcgcc agcagcgccg
     7801 cacccgccat cgcatacgtt ttggtgaacg ccaacgggcc aaacagacgc ccttcctgcc
     7861 cttccagggt gaagatcggg ataaacgaca acgtgataat cagcagactg ataaacagcg
     7921 ccggccccac ttcaacagac gcatcggtga tcacctgcca gcgcgtttta ttatccagcg
     7981 tggcgtcagg atgctggtgc tgccactctt ccagccgttt atgcgcattc tcgatcatga
     8041 cgatagcagc atcgaccatc gccccaacgg caatcgcaat gccacccaac gacataatat
     8101 tggcattcag cccctggaag tgcatgacaa taaaagcaat acacaacccc agcggcaacg
     8161 aaataatcgc caccagcgcc gagcgcacat gccagagaaa cagcgcacag actaccgcca
     8221 ccacaataaa ctcttccagc aacttgccgc tgaggttgtc gatggcccgg tcaatgagct
     8281 ggctgcgatc gtatgttgta actattttca cgccttccgg cagactgctt ttcagcgttt
     8341 ccagtttgtc cttcacggcg gcgatcactt cacgggcgtt tttgccggag cgtaggatca
     8401 ccaccccgcc cgcaacttcg ccttcgccgt tcagttcggc aatgccccgg cgcatctccg
     8461 ggccaatctg gaccttcgca acatcgcgca gataaacggg cacgccattt tcactggctt
     8521 ttaaaacgat gtgattaaag tcgtcgagcg tttgcagata gccgctggcg cgcaccatat
     8581 attccgcttc cgccagttcg atcgacgaac cgcccgcttc ctggtttgaa gcatccagcg
     8641 cgctttttac ttcggcgaga ctgatgccat attgcgccag gcgctgagga tcgataacca
     8701 cctgatactc tttcaccaca ccgcccaccg acgccacttc cgcaacgtca ggaatggttt
     8761 tcagctcata tttgagaaac cagtcctgca atgagcgtaa atcggcaaga tcgtgcttac
     8821 cgctgcgatc caccagtgca tattcataga tccagccaac acccgtggca tctggcccca
     8881 gctcggcgct gactcccgca ggcagcttac cctgtacctg gttgaggtac tccagcaccc
     8941 gcgaacgcgc ccagtacgga tcggtgccat cttcgaaaat gacatacaca taagagtcgc
     9001 caaactgcga gaaaccacgc acagtcttcg cgccaggcac cgacaacatg gtggtggtta
     9061 gcggataagt cacctgattt tcaacgattt gcggtgcctg gccgggatag ctggttttaa
     9121 taatcacctg cacatcggag agatccggca gcgcatccac tggcgtatta atgatggtcc
     9181 aggtgcccca gatacttaga aacaacgcgc ccatcagcac caaaaaacgg ttcgccaccg
     9241 agcgacgaat aatccattca atcattggtt attccctcaa tgcgcatggg tagcactttc
     9301 agagcgcatc cgctccagtg cgccagaaat attggcttcg gaatcaatca ggaacaggcc
     9361 gctggaaacc accttttcac cttccgccag accagagcgt aatgcggtga caccttgcga
     9421 tgcctggaaa acggcaacgc gttttggtac aaagcgcccg tcggcatcaa cggtaatcac
     9481 ccgctgttcg ttgccggtat caatcagcgc ctgtgacgga atgagcagca tcggttcgct
     9541 ggcggtgttg agttgcaacc aggcgttcat gcccggtttt agcgcctcgt cggcgttgtc
     9601 gacttccaga cgcagctgca gcgtgcgggt cgcggcatcc acgccaggta gtagcgtcca
     9661 tttgcggatg gtgagtgttt tatcaggtcg cgccggaacg gtgagcgtaa actgcgaggc
     9721 atctttcacc agccaggcga tggactccgg gatcgcagca gtgacccaca ccgggtccat
     9781 accctgaatt ttcgctacca cgttatcttt ggcgatattc attcccgcgc gcagatcaaa
     9841 cgcggtgatc acgccatcaa tgggtgcttt gagcgtaaag cgagtctgga ttttttgcgt
     9901 ggcgatcagg cggcgaatat ccgcctccgg cattcccgcc agtcgcagcc gctcaagaat
     9961 gccttcagtc tgggtcgccg taccgccggt ttcgcgcagc agtaaatact cactctgcgc
    10021 ttccacccag tcaggaatgg tcaggtcgag aagcggtgcg cccttttgca ctttatcgcc
    10081 cacggtaagc ggatacacct tgtcgataaa cccggcagcg cgagcctgca caatggcata
    10141 ctgatactcg ttgtaactga cattcgccgg gaaactctgg gcaaaagtca gcggcccgcg
    10201 cgtgacggta gccgttttca cccccagatt ctgcgtctga gtcgggtcaa tgcgcacacc
    10261 agacgcagaa ctctcttcat cggcatattt cggcaccaga tccatatcca taaacggcga
    10321 tttacctggt ttatcgaacc gcgtattggg atacatcggg tcgtaccaga ataagatttt
    10381 acgttctgcg gtcgacgttt tttctgcggg cggttcagcc cggtcaaacc aggtaaaacc
    10441 tgccgcagaa ataataccgc ccgcgatcat gctgccgata ataagcgcga tttttttcat
    10501 ctcattaaac ctgggttact ggctgacttt aatatcctgt aataaagaaa ggttgccctg
    10561 ctggacaaaa ttaaacgcca ctttgtcgcc agttttaatt tcactcattt tcgtctgcgg
    10621 agtgatggta aagcgcatag tcatctccgg ccagttcacg gcagcaatcg gatcgtgatg
    10681 gatggtaatt tttttgcttt ccagatcaac accttttacc acgccggtgg cactaatgac
    10741 ctgtggttgt gcttcgctca tggtttcatg atgatgttcg ttagcctggg cattaaagcc
    10801 aataacggta aacagactga acattgcgac ttgcagtgct tttttcattt attctctcct
    10861 ggagttaaaa gttattgctg ccaaccgcca ccaagtgcgg tatataaaga aatttcgtta
    10921 acctgacggg cataattcag gtcaagtaaa gtttgtcggg ttgcaaataa cgaacgctcg
    10981 gcatccagca cttccagata acttaccgcg ccgtgctgat ataatgcccg cgcccgttgc
    11041 aaagtaattt gcagcgatgc cagataacgc tgctgggcgc taatttgatc gttcaggctt
    11101 tgacgtaatg ccagcgcatc tgccacttct ttaaaggcgt tctggatttt ctgttcataa
    11161 ttcactaccg actgctgctg gcgaatttcg gcgatatcca gattggcctg gttgcgtccg
    11221 gcattaaaaa tggggatctc aattttagga ataaaattcc acatcccgct gctggcgtta
    11281 aataatgacg acagatcgct gctggcggtt gatattccgc tggtcaggct gatagacggg
    11341 aaaaatgctg cacgtgcagc accaatattg gcattagccg ccattaacgc gtgttcagct
    11401 tccataatat ccgggcgctg caataagatt tgcgacgaca agcccgccgg taatttaacg
    11461 ctttgcaggc tgtcgctgtt tactgtctgc gcatgcggca gcttgccgta gcttcccagt
    11521 aacagttgca atgcattatt cgcctgcgcc agttccccct gacgtttagc gatgtcgctg
    11581 cgggtacttt caatcacccc gcgagcctgt tccagcgcca gaacattact gctacctgtc
    11641 aacagttgtt tttcgacaaa cgcatatgac tgctgataat tacgcagcgt ttcttcggct
    11701 atttgcaatt gcgcatacgc cagttgctga ttgaaatagc tttgcgcgac attagaaacc
    11761 agcagaatat gcaccgcgcg ctgagcttcc tcagtggcta aataattttg tcgctcggct
    11821 tcgctcatgt tctttaagcg cccgaaaaaa tcgaggtcaa agctggcgtt aaggccagta
    11881 gaaaactccc gcgtcgtggc tgaatcgcct ttaagattgc cgctccagct gccgctgccc
    11941 tcgccattga gctgtgggta gcggtcggca tcggtcagac gatattgcgc ccgtgcctcc
    12001 tgcactttca gcgtcgccat gcgcaaatcc cggttattca ccagcgcctc gctaatcagc
    12061 gtcttcacct gattatcaac aaaaaaggtg cgccagcccg cgttctgata gttatctgct
    12121 gcgttaacga ggccgttctg gctgagtgag aactgctgcg gtacgggcat tgccgggcgc
    12181 tgataatccg gtgccagtga acaaccggtt agcgcaaggg ccacacaaaa tggcagaagt
    12241 ttacaaggag acataggctc ataatttctg gtgattttat accgccaact ttactcgcca
    12301 ggctctgatt ttccggtgac aggaaaatga caaaattgtc attttgccaa taagcgattg
    12361 ccatctgatc ccgctactct agaattgccc gggcaacatg tggaggaaat atgaaactgt
    12421 tgattgtcga agatgaaaag aaaaccggag aatacttgac caaagggtta accgaagccg
    12481 gttttgtggt cgatttggcc gacaacgggc tgaatggcta ccatctggcg atgaccggag
    12541 attatgacct gataatcctc gatattatgc tgccggacgt gaacggctgg gatatcgtgc
    12601 gcatgttgcg ctccgccaat aaagggatgc cgattctgtt gctaaccgcg cttggcacca
    12661 ttgaacatcg cgtcaagggg ctggagttgg gggcagatga ctacctggtg aagccattcg
    12721 cttttgctga actgctggcg cgggtgcgca cattactgcg gcgcggggcg gcggtgatta
    12781 tcgaaagtca gtttcaggtt gccgatctga tggtcgatct cgtcagccgc aaagtcactc
    12841 gcagcggcac gcgcatcact ttgaccagta aagagtttac tctgctggag ttttttctcc
    12901 gccatcaggg cgaagtgctg ccccgctcgc ttatcgcctc gcaggtatgg gacatgaatt
    12961 ttgacagcga cactaacgcc attgatgtgg cggtgaagcg gctacgcggc aaaatcgaca
    13021 acgactttga gccgaagctg attcagaccg tgcgcggcgt gggctatatg cttgaggtac
    13081 ctgatggtca ataagccatt tcagcgcccg ttttcgctgg caacccgcct gacctttttt
    13141 atcagcctgg ccaccatcgc ggcgtttttc gcctttgcat ggatcatgat ccactcagta
    13201 aaagtgcatt ttgccgagca ggatattaat gatttaaaag agattagcgc cacccttgaa
    13261 cgggtactaa atcaccctga cgaaacgcaa gcccgacgct taatgacgct ggaagatatc
    13321 gtcagtggtt attccaatgt gttgatttcc ctggcagata gccagggtaa aacggtgtat
    13381 cactcccccg gtgcgccgga tatccgcgag tttgcgcgtg acgccatacc cgataaagac
    13441 gcgcggggcg gtgaggtgta tctcctttcc ggcccgacga tgatgatgcc aggccacggt
    13501 cacgggcata tggaacacag caactggcgg atgattaact tgccggttgg cccgttggtg
    13561 gacggaaaac cgatttatac gctctatatc gcgctttcga tcgattttca tcttcattac
    13621 ataaatgatt tgatgaataa acttattatg accgcatcga tcatcagcat cctgatcgtc
    13681 tttatcgtgc tgttggcggt acataaaggt cacgcgccga tccgcagcgt cagccgtcaa
    13741 atccagaata tcacctcgaa agatctcgac gtccgccttg acccgcagac cgtgcccatt
    13801 gagctggaac agctggtact gtcgttcaac catatgatcg agcgtattga ggatgtcttt
    13861 acccgccagt ccaatttctc agcggacatc gcccacgaaa ttcgcacacc aattacgaat
    13921 ctcataacgc aaaccgaaat cgccctcagc cagtcgcgca gccagaagga gctggaagat
    13981 gtgctctact ctaatctcga agagctgacg cgaatggcga aaatggtcag cgatatgctg
    14041 tttctcgctc aggccgataa caaccagcta atccccgaaa agaaaatgct caacctggcg
    14101 gatgaaatcg gcaaagtgtt cgattttttc gaggcgttag cggaagatca cggcgtggag
    14161 ttgcgatttg ttggcgacga gtgccaggtt gcgggcgatc cgctgatgct gcgtcgggcg
    14221 ttaagcaacc tgctttcgaa cgccctgcgt tatacgccac ccggagaggc tattgtagtg
    14281 cgctgccaga cagtcgatca tctggtgcaa gttatcgtcg aaaaccccgg tacgcccatt
    14341 gcgcccgagc acttaccgcg attgtttgac cgtttctacc gcgttgaccc ctcccgccaa
    14401 cgaaaaggcg aaggtagcgg cattgggctg gcgatagtga aatcgattgt tgtcgcgcat
    14461 aaaggcacgg ttgcagtaac gtcagatgcg cgggggacaa ggtttgtgat tgtgttgccg
    14521 gagagggggt gattattaag ttcaaattca gggcaagggt ttacggcgtt tttcgctaaa
    14581 tttccatcat tataaacaa
//
LOCUS       DAZUCE010000123        14523 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZUCE010000123 DAZUCE010000000
VERSION     DAZUCE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.123"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..185
                     /locus_tag="ACHY03_003131"
     CDS             <1..185
                     /locus_tag="ACHY03_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906329.1"
                     /translation="RIGRCMGYLQVAAEIEALASGADKDSVVREALLRDFDNPPFKKV
                     PAYWLHPGLTYLKVRI"
     gene            222..755
                     /locus_tag="ACHY03_003132"
     CDS             222..755
                     /locus_tag="ACHY03_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS3906330.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            912..1094
                     /locus_tag="ACHY03_003133"
     CDS             912..1094
                     /locus_tag="ACHY03_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906331.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            1109..1240
                     /locus_tag="ACHY03_003134"
     CDS             1109..1240
                     /locus_tag="ACHY03_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906332.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            1463..1648
                     /locus_tag="ACHY03_003135"
     CDS             1463..1648
                     /locus_tag="ACHY03_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFS3906333.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPVQAPCVKPPPPPAWIMQPA
                     PDWQTQLNGIISPSESG"
     gene            2191..2697
                     /locus_tag="ACHY03_003136"
     CDS             2191..2697
                     /locus_tag="ACHY03_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS3906334.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVLYDVKTVL
                     EIDRERRQHNQRTPDDGGELEERLLRARAELTEEQAVAQKLKNQVTEGKLIDSDFCVF
                     ALSKLAMALSSTLDSIPLSMQRQFPDLTPRHIDHLKTLIAKGANQCARAGDKLPDLLD
                     EYIRATTE"
     gene            2669..4597
                     /locus_tag="ACHY03_003137"
     CDS             2669..4597
                     /locus_tag="ACHY03_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFS3906335.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVVPMTAVEWADQNYYLPKDS
                     SYGDGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVHYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            4581..4787
                     /locus_tag="ACHY03_003138"
     CDS             4581..4787
                     /locus_tag="ACHY03_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFS3906336.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            4784..6376
                     /locus_tag="ACHY03_003139"
     CDS             4784..6376
                     /locus_tag="ACHY03_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS3906337.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYHGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQQDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIDSGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            6366..7871
                     /locus_tag="ACHY03_003140"
     CDS             6366..7871
                     /locus_tag="ACHY03_003140"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFS3906338.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEFMTGDKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPSEVRQDMQQRIDAAHRMFAEKVVMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPD
                     MTVEKARPILAAAPLADAGPSLRDQIMVLDEAKGAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTALFERFMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            7908..8255
                     /locus_tag="ACHY03_003141"
     CDS             7908..8255
                     /locus_tag="ACHY03_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS3906339.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
     gene            8313..9341
                     /locus_tag="ACHY03_003142"
     CDS             8313..9341
                     /locus_tag="ACHY03_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS3906340.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTSAEGIRCYGAIQDAQALSEGVVASSRYPKHWMTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            9393..9776
                     /gene="gpFI"
                     /locus_tag="ACHY03_003143"
     CDS             9393..9776
                     /gene="gpFI"
                     /locus_tag="ACHY03_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS3906341.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIALRVSEWEEELAA
                     SREGIMHSDESGSDQNDTDDGEQLNNTDSPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTAEYA"
     gene            9769..10122
                     /locus_tag="ACHY03_003144"
     CDS             9769..10122
                     /locus_tag="ACHY03_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFS3906342.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            10138..10713
                     /locus_tag="ACHY03_003145"
     CDS             10138..10713
                     /locus_tag="ACHY03_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3906343.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            10710..11105
                     /locus_tag="ACHY03_003146"
     CDS             10710..11105
                     /locus_tag="ACHY03_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFS3906344.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAEIPALAGMITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            11113..11862
                     /locus_tag="ACHY03_003147"
     CDS             11113..11862
                     /locus_tag="ACHY03_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3906345.1"
                     /translation="MATPNPLAPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGEITP
                     VTGITVTPLTGNVAKGQSTTLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            11879..12301
                     /locus_tag="ACHY03_003148"
     CDS             11879..12301
                     /locus_tag="ACHY03_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFS3906346.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRYDADPEQIGKAEATEPVTSKKHSKAS"
     gene            12328..12741
                     /locus_tag="ACHY03_003149"
     CDS             12328..12741
                     /locus_tag="ACHY03_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFS3906347.1"
                     /translation="MGRADWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGERD
                     ISPSADVVDVREDDVALMMASAGISGGVRYVPASG"
     gene            12722..>14523
                     /locus_tag="ACHY03_003150"
     CDS             12722..>14523
                     /locus_tag="ACHY03_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFS3906348.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASAV
                     EQGLSRQALAAQKAGMSVGQYKAAMRMLPMQFTDVATQLAGGHNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAISLPMAGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTAERMLTLSRAGQAAGLTFNQAGESLAALVSAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPDTAQEMLIKAETAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQAELNKALRDGKILQADYNTLMASAKKDYEST
                     LKKPKSSGVKVSAGERQEDRAHAAMLALETELRTLEKHSGANEKISQQRRDLWKAESQ
                     YAVLKEAATKRQLSGQEKSLLAHEKETLEYKRQLAELGDKVEYQKRLNELAQQAVRFE
                     EQQSAKQAAISAKARGLTDRQAQRESEEQRLRDV"
BASE COUNT         3424 a         3438 c         4486 g         3175 t
ORIGIN      
        1 agcgcattgg tcgttgcatg gggtatctgc aggtagccgc tgagattgaa gcgctggcca
       61 gtggtgcgga caaggattca gttgtgcggg aggcacttct tcgtgatttt gataatccgc
      121 cctttaaaaa agtgccggct tactggcttc atcccggact gacttatctg aaagtgcgta
      181 tttagtgggc ctgggacagc ggctgaatat ttaatatatc catgaacaca aaaatcaaat
      241 acggcctgtc ggctgccgtt ctggcgttga ttgccgcagg ggcttctgcg cctgaaatcc
      301 tcgaccagtt tctggatgaa aaagaaggta accacaccac ggcataccgt gatggcgcgg
      361 gtatctggac catctgccgt ggagccaccc gggtggatgg taagcctgtt attcctggca
      421 tgaagctgtc gaaggggaaa tgcgaccagg ttaacgccat tgagcgtgat aaggcgctgg
      481 catgggtgga gaaaaacatc agagtgccac tgactgaacc ccagaaagcg ggtattgcgt
      541 cattctgtcc ttacaacatt ggcccgggta agtgtttccc gtcgacgttt tataaacgaa
      601 ttaatgcagg cgatcgcagg ggagcgtgtg aagcgattcg ctggtggatt aaggacggtg
      661 gcagagactg ccgtatccgc tcaaacaact gttatggtca gatatcccgt cgtgaccagg
      721 agagcgcgct ggcgtgctgg ggaatcgaca gataagcaga atattttgct gaaaaatgac
      781 gttggccaac gcggacggat aacacgaaat cctgcgaact ggcaaaacct aagtgaataa
      841 aagtaaaaac cccgtttgtt ggcagcaagc ggggttttgt gtttctgacc ttggataagg
      901 caagggagaa catggaaaag tataaacgaa ttctgttgag gttgactatg aaaaacggcc
      961 ttgaactgaa agcgccagta actgatgaca tcagcagagc actggctttt gctattaagt
     1021 gggtggcggt cggtatcgct gtgtctccga tgctgtatgg gctggcaaaa ctggtcattg
     1081 cgttgaaatc gtgaagggag gattaagcat gtcagacaaa ctcataatgc tggcgaagat
     1141 cctctgtgta atcgtcggta tttcattttc actaatgctg gttgctattt ttcttttcct
     1201 gccctggatg atgttgtctt cgtcggggat gctggggtga gcataaaccg aatgctttcc
     1261 gcgtttaccg ttattctgct ggtggcctgt ggtgcgctta gtctggggct gaatcattac
     1321 cgcgataacg ccatcaccta caaagcgcag cgcgataaaa aagccagaga gctggaacag
     1381 gcgaatgcca ccattactga catgcaggtg cgccagcgtg atgttgctgc gctcgatgca
     1441 aaatactcga gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt
     1501 gccgctggtc gtaagcgcct gcggatcaac gccacctgtc caggctccgt gcgtgaagcc
     1561 cccaccacct ccggcgtgga taatgcaacc ggcccccgac tggcagacac agttgaacgg
     1621 gattatttca ccctcagaga gcggttgatg atgatgcaga agcagctgga aggggcgcag
     1681 gaatatatcc gcactcagtg cctgaaataa gttttgctga tgcgccgtat cgtcgccgta
     1741 ttcccgtatt aacagagacc gcagcccgac agggagactc ctctgcgaga gtgtgcgggg
     1801 ataatcaaaa acgatacaca ccggggttta ccgcgttaac ggagcgcggc gttgtcccct
     1861 catagtcgcc tgtccggtgc gatggtggaa gaaactggat ttgttgcaac tgataaccat
     1921 tatcattttc tcgggtcctt tccggcatat ggacccgtta cggggcggcg acctcgcgcg
     1981 ttttcactat ttatgagatt ttttgagggg gtggttgttg tttaattgtt tggtatatct
     2041 acttgataag taaggtgaaa ataaaatcaa tacaacaacc ttacgatgtg ttttgatgtc
     2101 gtcaatgcga aaaatgtcaa tgatatcaaa tggttttgca aaaacacatg gttgttgttt
     2161 cgctttttat cgatgactta tggagaggag atggcctttt tattgaataa aagtgatatg
     2221 gcctcctcca tcggtatctc tgttcaggca tttgataaat ggggcgttcc tcctgttgaa
     2281 cgtcggggga gggaagtttt atatgacgtt aaaactgtac tggagataga tcgcgagcgg
     2341 cgacaacaca atcagagaac acctgatgac gggggagaac tggaggaaag gctgcttcgg
     2401 gccagagctg aactgacaga agaacaggct gtagctcaaa aacttaaaaa tcaggtaacc
     2461 gaaggtaagc tcatcgattc agacttctgc gttttcgccc tcagcaaact ggcgatggca
     2521 ttgtccagta cgcttgattc cattccgtta tccatgcagc gacagttccc ggatttaacg
     2581 ccacgtcata ttgaccatct gaaaaccctt attgcaaagg gcgcaaatca gtgtgcgcgg
     2641 gcaggggata aattaccgga tttactcgat gaatatatca gagcaacaac tgaataatat
     2701 gatggctgcc gtttcggttg cgctgcagcc tctggtcaga gttgtaccga tgacggcagt
     2761 tgaatgggct gatcaaaatt attatttgcc taaagactcc tcatacggtg acggagaatg
     2821 gaaaacgctg ccattccaga tcgccatcat gaacagcatg gggaatgatc agatccgcac
     2881 tgttaatctg attaaatctg cccgtgttgg ctatacaaag atgttgctgg gggtggtcgg
     2941 gtattttatt gagcataaat cccgaaacag tctgcttttt cagcccacgg attctgccgc
     3001 tgaagatttt atgaagtctc acgtggaggc gacgattcgg gacgtgccat gcctgaaaga
     3061 tctttccccg tggctgggtc gtaaacatcg tgacaatact ctcacgctga aacgcttttc
     3121 atcgggtgtg ggtttctggt gcctgggcgg cgctgccgcc aaaaactacc gtgaaaaatc
     3181 cgtggacgtg gtctgctatg acgaactttc ctcgttcgag ccggatgttg aaaaagaggg
     3241 ctcgccaacc ctgctggggg ataagcgtat tgagggctcg gtatggccaa aatctattcg
     3301 cggctcgacg cctaaaataa aaggcacctg ccagatcgaa aaagcggcca acgagtcggc
     3361 gcatttcatg cgtttttatg tgccctgccc gcactgtggg gaggagcagt atctgaaatt
     3421 tggcgatgaa tccacgcctt ttggccttaa atgggagaag gacagccccg aaagcgtttt
     3481 ctatctctgt gaacatcatg gctgcgtgat ccatcagtct gagcttgacc agagcaacgg
     3541 gcggtggatc tgtgaaaaca cgggcatgtg gacccgtgac ggcctgacgt ttttcagcgc
     3601 tgcgggtaat gaaattccgc cgccgcgctc catcactttc catatctgga cggcgtacag
     3661 tccgttcacc acctgggtac agatagtcta tgactggctg gatgcactga aagatcccaa
     3721 cggcctgaaa acttttgtga acaccacgct gggcgagacc tgggaagagg ccgtgggcga
     3781 aaaactcgat caccaggtgc tgatggataa ggtcgtgcat tacacggcag cggtgcctgc
     3841 ccgggtggtt tatctgacgg cgggcattga ctcgcagcgt aaccgttttg agatgtatgt
     3901 ctggggatgg gctccgggag aggaagcctt tctggtggat aaaatcatca ttatggggcg
     3961 tcctgatgag gaagagacgc tgttacgtgt ggatgcggcg atcaacaaaa aataccgcca
     4021 tgcagacggt accgaaatga ccatttcccg tgtctgctgg gacaccgggg ggatcgatgg
     4081 tgaaattgtt tatcagagat caaaaaaaca cggtgttttc cgggtgctgc cggtaaaagg
     4141 cgcatctgtc tatggcaagc cggtgatcac catgccaaaa acccgcaatc agcggggcgt
     4201 gtatctgtgt gaagtgggga cagacaccgc aaaagaaatt ctctatgccc gtatgaaagc
     4261 cgatcccacg cctgcggatg aagccacgtc gtatgccatc cgttttcctg atgatccgga
     4321 gattttttcg cagacagagg cgcagcaact ggtggcggaa gaactggtgg agaagtggga
     4381 aaaaggaaag atgcgtctgc tgtgggataa caaaaagcgg cgtaacgaag cgctggactg
     4441 cctggtgtat gcctacgcgg cattacgtgt gtccgtgcaa cgctggcagc ttgatctggc
     4501 tgtactggca aaatcccggg aagaagagac gacccggcca accctgaaag aactggcagc
     4561 gaagctgtcc ggaggagtga atggttacag tcgctgaact gcaggcgctg cgtcaggcgc
     4621 gccttgattt attaaccggt aaacgggtgg tgtctgtcca gaaagatggt cgcagaattg
     4681 aatatacggc ggcttctctg gatgagctta accgtgcgat caatgatgcg gagtcggtac
     4741 tggggacaac ccgccgtcgc cgtcgtccgc tgggagtgag gttatgaaac gaacgcctgt
     4801 cctgattgat gtgaacggcg ttccgcttcg ggagagtctc agctaccacg ggggcggtgc
     4861 aggatttggc gggcaaatgg cggagtggtt gccaccggcg cagagtgccg atgcggccct
     4921 gctgcccgcg ttgcgtctgg ggaatgcccg tgcagatgat ctggtacgca ataacgggat
     4981 agcggctaat gcggtggccc tgcataagga tcatattgtc gggcatatgt ttcttatcag
     5041 ctaccgtccg aactggcgct ggctggggat gcgggagact gcggcaaaaa gttttgtcga
     5101 tgaggtggag gcggcctggt cggaatacgc agaagggatg tttggcgaga tcgacgtgga
     5161 agggaaacgc acgttcacgg aatttatccg tgaaggtgtg ggcgttcatg cctttaacgg
     5221 cgaaatcttt gtgcagccgg tctgggatac ggaaaccacg cagttattcc gcacgcgttt
     5281 taaagccgtg agtccgaaac gggtggacac gccagggcac ggtatgggga accgctttct
     5341 gcgggccggt gtggaggttg atcgatatgg ccgtgccgtt gcgtaccata tctgtgagga
     5401 tgattttccg ttctctggta gtggacgatg ggaacggatc ccgcgtgaac ttcccaccgg
     5461 gcgtccggcc atgctgcata ttttcgagcc ggtggaggac gggcagaccc gtggggccaa
     5521 tcagttttac agcgtcatgg aacggctgaa gatgctcgat tccctgcagg caacacagct
     5581 tcagtcggcc attgttaaag ccatgtatgc agcgacgatt gaaagtgaac ttgataccga
     5641 aaaggccttt gaatatatcg cgggggcacc gcagggtcag caggataatc cgcttattaa
     5701 tattctggag aagttctcca gctggtatga cacgaatcat gtgacgctgg gcggtgtcaa
     5761 aattccgcac cttttccccg gtgatgatct gaaactgcag accgcgcagg attcagacaa
     5821 tggattttcg gcgcttgaac aggcgctgct gcggtatatc gccgccggtc ttggcgtttc
     5881 ctacgaacag ttgtcccgtg attactcgaa ggtcagttat tcaagtgccc gcgcctctgc
     5941 caatgagtcg tggcgctatt ttatgggacg acgaaaattt attgcggccc ggctggccac
     6001 gcagatgttt tcctgctggc tggaagaggc acttcttcgg gggattatcc gtccgccacg
     6061 ggcacgtttt gatttttatc aggcgcgatc agcctggtca cgggccgagt ggattggtgc
     6121 cggaagaatg gccattgacg ggctcaagga ggtccaggaa tcggtgatgc gtattgattc
     6181 cggactgagc acgtatgaga aagagctggc gctgatgggt gaggattatc aggacatttt
     6241 ccgccagcag gtcagggaat ccgcagagcg ggaaaaagcc ggactctcac gtccggtgtg
     6301 gatagcgcag gcgtatcagc agcagatagc ggagagccgc aggccggaag aggagacaac
     6361 accacgtgag acgtaatctt tcacacatta ttgccgcagc attcaatgaa ccgctgcttc
     6421 tggagcccgc ctatgcgcgg gttttctttt gcgcgctggg gcgcgagatg ggggcagcaa
     6481 gtctttcggt accacaacag caggtacagc ttgatgctcc cggaatgctg gctgaaacgg
     6541 atgagttcat gaccggagat aaacgaccgg cccgtgttta ccgggtggtg aacggtattg
     6601 ctgtactgcc ggtgaccggc acgctggtgc accggctggg tggcatgcgg ccattttccg
     6661 gaatgacagg ctatgacggc attgttgcct gtcttcagca ggcaatggcg gacacctctg
     6721 tccggggcgt actgctggat attgacagtc cgggcgggca ggccgccggt gcgtttgact
     6781 gcgctgacat gatttaccgc ctccggcagc agaagccggt ctgggcactg tgtaatgaca
     6841 cggcctgttc tgcggccatg ctgctggcgt cggcctgctc ccgacggctg gttacccaga
     6901 catcccgtat cggctccatt ggcgtgatga tgggccatgt cagctatgcc ggtcatctgg
     6961 cgcaggccgg agtggatatc acgctgattt atgccggggc gcacaaggtg gatggcaatc
     7021 agtttgaagc gttgccgtca gaggttcgcc aggacatgca gcagcggatt gatgcggcgc
     7081 accggatgtt tgccgaaaaa gtggtgatgt atacggggct gtctgtggaa gctgtcacgg
     7141 ggacagaggc tgccgttttt gaaggtcagt ccggtattga ggccgggctg gcggatgaat
     7201 taatcaatgc gtcggatgcc atcagcgtga tggctgcggc gctgaacaca catgatacag
     7261 gaggcactat gccgcaatta actgcaacgg aagccgccgc gcaggagaac cagcgagtga
     7321 tggggatcct gacgtgtcag gaagcgaaag gacgtgaaca gcttgccacg atgctggcag
     7381 gacaacaggg catgagcgtt gaacaggccc gggcgattct ggccgcggca gcaccgcagc
     7441 agccggtggc atccgcgcag agtgaagccg atcgcattat ggcgtgtgaa gaagctaaag
     7501 gtcgtgaaca actggcggca acgctggcgg cgatgccgga tatgacggtg gaaaaagccc
     7561 gcccgatcct ggcggctgca ccactggcgg atgccggacc ctcactccgt gatcagatta
     7621 tggttctgga tgaggcaaaa ggggctgagg cgcaggctga aaaactggcg gcgtttcccg
     7681 gaatgacggt ggaggctgcc cgcgacattc tgtcctcatc gccggataaa gcagaaccgg
     7741 tttctgcatc cacaaccgcc ctgtttgaac ggttcatggc gaaccattca ccggcagccg
     7801 tgcagggtgg tgtgtcacag acgtcggcag acggtgatgc ggacgtgaaa atgctcatgg
     7861 ccatgccatg aagtcagtgc tgaccatcaa tatgaggttt ttacaatatg gtaacgaaaa
     7921 ccatcactga acaacgtgca gaagtacgta tttttgccgg taatgatccg gctcataccg
     7981 ccacaggcag cagcgggatt tctcaggcaa caccggcact gacgcccctg atgctggatg
     8041 aagccagcgg gaaactggtg gtctgggacg gacagaaagc cggtagtgcg gctggcatac
     8101 tggtactgcc gcttgaaggc acagagacgg tgctgacgta ttacaagtcg gggacctttg
     8161 cgacggaggc aatccgctgg cctgaaagtg tggatgaaca caaaaaggcc aacgcctttg
     8221 tcggcacagc cctgagtcac gcggcgctgc cgtaacacgt tatcaggcca ccgcgttggc
     8281 ctgactgatt tctgaatgaa aggaactgat ttatgggatt gtttacgacc cgccagttac
     8341 tcggttatac cgaacaaaaa gtgaaatttc gtgcgctgtt tctggaactg tttttccgcc
     8401 gtacggtgaa tttccacacc gaagaggtga tgctggacaa aattaccgga aaaacgccgg
     8461 tggcagccta tgtctccccg gttgttgaag gaaaagtgct gcgtcatcgt ggtggtgaaa
     8521 cccgcgtgtt gcgtccgggc tacgtcaagc cgaaacacga atttaattac cagcaggcgg
     8581 tggagcgcct tcccggtgaa gatccggcgc aactgaacga cccggcctac cgtcgtctgc
     8641 gtatcatcac tgataacctc aaacaggaag agcatgccat tgtccaggtg gaggaaatgc
     8701 aggcggtgaa tgccgtgctg tatggcaaat acaccatgga aggggagcag tttgatactg
     8761 tcgaggtgga tttcgggcgc tctgaaggaa ataacattga gcaggctgac ggtaaaaaat
     8821 ggtctgagca ggaccgtgat acgtttgatc cgacgcatga tattgacctc tactgcgatc
     8881 aggccagcgg tcttgtgaat atcgccatta tggacggtac ggtctggcgt ctgctgaatg
     8941 gctttaagtt gttccgcgaa aaactggata cccgtcgcgg ctcaaattca caactcgaaa
     9001 cggcagtgaa agatctgggc gcagtggtgt ccttcaaggg gtattacggc gatctggcca
     9061 ttgtggtggc gaaaacgtct tatgtggcag aggacggtac cgaaaaacgt tatctgccgg
     9121 agggcacgct ggtcctggga aatacgtcag cggagggcat ccgttgctat ggtgccattc
     9181 aggatgcgca ggcgttgtcc gaaggtgtgg tggcctcttc ccgttatccg aaacactgga
     9241 tgaccgtggg cgatccggcc cgtgaattca ccatgacgca gtccgcgccg ctgatggtgc
     9301 tgccggatcc ggatgagttt gtggtggtac aggtgaaata atccgggagc gggggcgaaa
     9361 tgcccccgtg tcttttttca cagggggctg agatggcaac aaaagaagaa aatctgaatc
     9421 gtcttcgtca actggctggt ctgctggggc gcgaggcgga tatgtcgggg agtgctgcgg
     9481 atattgctct gcgtgtgtct gagtgggaag aggagcttgc agcttcccgg gagggcatta
     9541 tgcactctga tgagagcggg tctgatcaaa atgacacaga cgatggtgag cagttgaaca
     9601 acacggattc tccggatgat gttaaagccg tccgggtacg gaagtgcctg caagtaatgg
     9661 ggtattgccc ggagacaggt cgtcccgttg agctggcgtt acggggtatg cgtgttctgg
     9721 tgccatcatc actggcaacg gccatgatac agcacggaac ggctgaatat gcgtgatttt
     9781 cagaatgcct ttgatgctgc cctcgccggg gtggacagta cgatcgttga agtgatgggg
     9841 atccgtgcgc agttcacctc cggtgcacag cgtggcggcg aagttcaggg ggtttttgac
     9901 gatccggagt cgctgggttt tgccggtggc ggggtccgta ttgaaggaag cagcccgtca
     9961 ttatttgtgc ggacggatac ggtgcgtgct gtgcggcgtg gtgacacgct gaccattaac
    10021 ggcgagatgt tctgggtgga tcgtgtttct ccggatgacg ggggcagctg ttatctctgg
    10081 ctcaaccgtg ggcaaccacc cgcagttaac cggcgacgat aaacgcaggg tgaaattatg
    10141 gcgataaaag ggcttgatca ggcgattgat aatctgagcc gggttcgtaa aaacgccatt
    10201 ccggcggctt cagcaatgac gattaaccgc gtggccacaa cggcgattaa tcagtcttca
    10261 tcacaggttg cccgggagac aaaggtacgc cggaaactgg ttaaggaacg gtccagactg
    10321 aaacgggcga cggtcagaaa tccgaatgca aaaattatcg ttaaccgcgg tgatctccca
    10381 gtgattaagc tggggatcag aatgctgggg cgtcgtccga acagcatact caaagccggt
    10441 cagcatcgtt atcagcgggc atttatccag cgattaaata atgggcgctg gcatgttatg
    10501 caacgtcttc cccaggccag atatgaggag ggcaatgacg acaagggcag gaaaaagcgt
    10561 aatcgccttc ccattcaggt ggtgaaaatc ccgatggcgg ccccactgaa acaggcgttt
    10621 gatgaaaacg ttgaccgtat ccgtcgtgaa cgcctgccca aagaactggc atatgcgctg
    10681 aaacaacaac tgaggattgc gataaaacga tgaaacatac tgatatccgt gcagccgtac
    10741 tggatgcact ggagaagcat gacaccgggg cgacgctgtt tgatggtcgc cccgctgttt
    10801 ttgaggaggc ggattttccg gcggtcgcgg tttatctgac ggatgcagag tataccggtg
    10861 aagagctgga tgctgatacc tggcgggcca cactgcatat tgaggtgttt ttaccggcac
    10921 aggtaccgga ttcagagctt gatcagtgga tggaaagccg gatttacccg gcgatggcgg
    10981 agatccctgc actggcaggc atgattacca cgatggttac gcagggctat gagtatcgtc
    11041 gtgatgacga tatggcattg tggagctctg cagatctgac ttattccatt acatacgaga
    11101 tgtgaggatg atatggcaac accaaatccc ctggcgccgg taaaaggttc cggcaccacg
    11161 ctctgggttt acaccggtaa gggcgatgct tatgcaaacc cgctgtcaga tgatgagtgg
    11221 acgcgtctgg caaaaataaa ggatctgacc cccggcgaga tgacggcgga atcctacgac
    11281 gataactatc tggatgatga ggatgctgac tgggtatcca ccgggcaggg gcagaaatct
    11341 gccggtgaca ccagttttac gctggcctgg aagcccggcg agaaagggca gcgtgatttg
    11401 attgcctggt ttgacagcag tgagacccgg gcctacaaaa tccgcttccc gaacggtacg
    11461 gtggatgtgt tccgtggctg ggtgagcgcc attggtaaag cggtgactgc caaagaagtg
    11521 atcacccgta cggtaaaaat caccaatatt ggtcgtccgt cgttggcgga ggatcgggga
    11581 gaaatcacac cggtcaccgg tattaccgtg acgccactaa cggggaatgt ggcaaaaggt
    11641 cagagcacca ccctgactgt ggccgttcag ccggaaggcg caacggataa aaccttccgc
    11701 gcaacgtcgg caaatcagaa tttcgccact attaccgtga aagggaacac gatcaccgtg
    11761 aaaggtgttg cggcaggtaa agcgcagatc cctgttgtca ccggcaatgg tgagtttgcg
    11821 gcggtggcgg agatcaccgt cacggatggc gctgcgggct gaggggagga gatcaagcat
    11881 gttcctgaaa acagaacaat ttgaatataa cggtgtgtcc gtcacgctgt cggagctgtc
    11941 cgcgctgcag cggtttgatt atataaagtt tgtttcagac gcagaacaac aggagacaac
    12001 gaagcatgat gccgtgcaca ttaaccagcg atatctggaa acggcatccc tgcttgtggc
    12061 gatgtcgcta tggcattccc attccctcaa aggcactctg gcctctccgg agacagagat
    12121 gcagcagatc cgccgtgaag tgatgctggg atggcctgct gatgcactga atcaggcaac
    12181 gaaccgggtg ctttatcttt caggtatgct ggataaccgg tacgatgccg atcctgaaca
    12241 aatcgggaaa gcagaagcga ctgagccggt aacatcaaaa aagcattcga aggcgagctg
    12301 aactttgtcc tgaaactggc gcgtgagatg gggagagccg actggcgcgc catgcttgcc
    12361 gggatgacat ccaccgaata tgccgactgg cgacgttttt actgcacgca ttattttcag
    12421 gatacccaac tggacgctca tttttccggg ctgatgtacg ccgtactcag cctgtttttt
    12481 ggcgatccgg atatgcatcc ggcggatttc agtctgcttg ctccagcgtg tgaggaagag
    12541 cagacagaga tgccggacga ggaagaaatg ctgatgcaga aagcgacagg agttgccgga
    12601 ggcgtccggt tcggagggga cggagagcgc gatatttcac cttctgcgga tgtggtggat
    12661 gtcagggagg atgatgttgc attaatgatg gcttcagcgg ggatttccgg aggtgtgaga
    12721 tatgtcccag ccagcgggtg atctggttat tgatttgagt ctggatgcgg cccggtttga
    12781 tgaacagatg gcccgggtac gtcgccattt ttccagtctg gaggcggatg ccagaaaaac
    12841 cgccagtgct gttgagcagg ggctgagccg ccaggcgctg gctgcacaaa aagccgggat
    12901 gtccgtcggg cagtataaag ccgccatgcg tatgctaccc atgcagttca ccgacgtggc
    12961 cacgcagctt gccggtggtc ataatccctg gctcatcctg ctgcaacagg gcggtcaggt
    13021 gaaggactcc ttcggcggga tgatccccat gttccggggg cttgccggtg cgatcagcct
    13081 gccaatggcc ggtgtcacct cgctggcggt ggcgaccggt gcgctggcgt acgcctggta
    13141 ccagggggat tccacgcttt cagcgtttaa taaaaccctg gttctttccg gtaatcagtc
    13201 aggactgacg gcagagcgca tgcttacgct ctccagagcc gggcaggcgg cagggctgac
    13261 gtttaaccag gcgggagagt cactggcagc cctggtcagt gccggtgtgc gtggtggtga
    13321 acagtttgat gccattaacc agagtgtcgc gcgttttgcg tctgcctccg gtgtggaggt
    13381 ggacaaggtt gcagaggctt tcggaaaact gaccaccgac ccgacgtcgg ggctgactgc
    13441 gatggcgcgc cagttccgta acgtgacggc agagcagatt gcgtatgttg cgcagctgca
    13501 gcgttccgga gacgaggccg gggcattgca ggcggcgaac gaggctgcca cgaaagggtt
    13561 tgatgaccag acccgccgcc tgaaagagaa catggggacg ctggaaacct gggcggataa
    13621 aacagggaag gcgttcaaat cgatgtggga tgccattctg gatattggtc gtcctgatac
    13681 cgcgcaggag atgctgatta aggcagagac tgcgtttaag aaagcagacg acatctggaa
    13741 tctccgcaag gatgattatt ttgttaacga tgaagcgcgg gcgcgttact gggatgatcg
    13801 tgaaaaggcc cgtcttgcgc ttgaagccgc ccgaaagaag gctgagcagc agactcaaca
    13861 ggacaaaaat gcgcagcagc agagcgatac cgaagcgtca cggctgaaat ataccgaaga
    13921 ggcgcagaag gcttacgaac gcctgcagac accgctggag aaatataccg cccgtcaggc
    13981 agaactgaac aaggctctga gagacgggaa aatcctgcag gcggattaca acacgctgat
    14041 ggcgtcggcg aaaaaggatt atgaatcgac gctgaaaaaa ccgaagtcgt caggtgtgaa
    14101 ggtgtctgcc ggtgagcgcc aggaagaccg ggcacatgca gccatgctgg cgcttgaaac
    14161 cgagctccgg acgctggaga agcacagtgg tgcgaatgag aaaatcagcc agcagcgccg
    14221 tgatttatgg aaagcggaaa gtcagtatgc ggtcctgaaa gaggctgcca cgaaacgaca
    14281 gttatccggg caggaaaaat ccctgctggc ccatgagaaa gagacgctgg agtacaaacg
    14341 tcagctggct gagctgggcg acaaggttga ataccagaaa cgcctgaatg agctggcaca
    14401 gcaggcggtg cggtttgaag agcagcagag cgcgaagcag gccgccatca gcgcaaaagc
    14461 ccgcggtctc actgaccgtc aggcacagcg ggagtctgaa gagcagcgtc ttcgtgacgt
    14521 gta
//
LOCUS       DAZUCE010000124        14513 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZUCE010000124 DAZUCE010000000
VERSION     DAZUCE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.124"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            245..1816
                     /gene="garD"
                     /locus_tag="ACHY03_003151"
     CDS             245..1816
                     /gene="garD"
                     /locus_tag="ACHY03_003151"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFS3906349.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1966..2301
                     /gene="prlF"
                     /locus_tag="ACHY03_003152"
     CDS             1966..2301
                     /gene="prlF"
                     /locus_tag="ACHY03_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFS3906350.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2301..2765
                     /gene="yhaV"
                     /locus_tag="ACHY03_003153"
     CDS             2301..2765
                     /gene="yhaV"
                     /locus_tag="ACHY03_003153"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFS3906351.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(2820..3629)
                     /gene="agaR"
                     /locus_tag="ACHY03_003154"
     CDS             complement(2820..3629)
                     /gene="agaR"
                     /locus_tag="ACHY03_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFS3906352.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPPVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3878..5158
                     /gene="kbaZ"
                     /locus_tag="ACHY03_003155"
     CDS             3878..5158
                     /gene="kbaZ"
                     /locus_tag="ACHY03_003155"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFS3906353.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETSL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5181..5654
                     /gene="agaV"
                     /locus_tag="ACHY03_003156"
     CDS             5181..5654
                     /gene="agaV"
                     /locus_tag="ACHY03_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFS3906354.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5665..6444
                     /gene="agaW"
                     /locus_tag="ACHY03_003157"
     CDS             5665..6444
                     /gene="agaW"
                     /locus_tag="ACHY03_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFS3906355.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6434..7312
                     /gene="agaE"
                     /locus_tag="ACHY03_003158"
     CDS             6434..7312
                     /gene="agaE"
                     /locus_tag="ACHY03_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFS3906356.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7330..7764
                     /gene="agaF"
                     /locus_tag="ACHY03_003159"
     CDS             7330..7764
                     /gene="agaF"
                     /locus_tag="ACHY03_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFS3906357.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELDRCHEECPVEEGI"
     gene            7761..8894
                     /gene="nagA"
                     /locus_tag="ACHY03_003160"
     CDS             7761..8894
                     /gene="nagA"
                     /locus_tag="ACHY03_003160"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFS3906358.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGIDDVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9245..10399
                     /locus_tag="ACHY03_003161"
     CDS             9245..10399
                     /locus_tag="ACHY03_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFS3906359.1"
                     /translation="MPENYTPAAAASGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10412..11272
                     /gene="kbaY"
                     /locus_tag="ACHY03_003162"
     CDS             10412..11272
                     /gene="kbaY"
                     /locus_tag="ACHY03_003162"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFS3906360.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11439..11915
                     /gene="agaB"
                     /locus_tag="ACHY03_003163"
     CDS             11439..11915
                     /gene="agaB"
                     /locus_tag="ACHY03_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFS3906361.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11954..12757
                     /gene="agaC"
                     /locus_tag="ACHY03_003164"
     CDS             11954..12757
                     /gene="agaC"
                     /locus_tag="ACHY03_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFS3906362.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12747..13538
                     /gene="agaD"
                     /locus_tag="ACHY03_003165"
     CDS             12747..13538
                     /gene="agaD"
                     /locus_tag="ACHY03_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFS3906363.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13539..14294
                     /locus_tag="ACHY03_003166"
     CDS             13539..14294
                     /locus_tag="ACHY03_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFS3906364.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPSTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGENLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTSIPASFLWLHSNFICLINT"
BASE COUNT         3532 a         3662 c         3759 g         3560 t
ORIGIN      
        1 gcaaatgcta atttaattaa taccatttga atattatttt gagcatatgc acataaggtt
       61 gcgcgctaaa gcacagattt gcgctttacc ttgccgggcg gcactgcaat ccctgaaatg
      121 attgacattg atcacatttc tgcgtttaaa ctcctgacat tcttatttca cccaatgaag
      181 tcatttattt ttaaatgaga ccaggtcctc attttaataa cccctggctg gagaatattg
      241 cacaatggcc aacatcgaaa tcagacaaga aacgccaact gcgttttata taaaagttca
      301 cgacacagat aatgtggcaa ttattgttaa tgataatggc ctgaaagcag gaacgcgttt
      361 tccggatggg ctggaattaa ttgaacatat tccccagggg cataaagtcg cattgctgga
      421 cattccggct aatggtgaaa ttattcgtta tggcgaagtg attggttacg ccgtgcgtgc
      481 aatcccacgc ggaagctgga tcgacgaatc aatggttgta ctaccggaag cgccgccgtt
      541 acacacgctg ccactggcaa ccaaagtccc ggaaccctta ccgccgctgg aaggatacac
      601 ctttgagggc tatcgcaatg ccgatggcag cgtgggcacc aaaaacctgc tcggcatcac
      661 caccagcgtc cactgtgtgg caggcgtggt ggactacgta gtaaaaatca ttgaacgcga
      721 tctgctaccg aaatacccga acgtcgatgg cgtggtgggg ctgaatcatt tgtacggttg
      781 tggcgtggcg attaacgcac cggcggcagt tgttcctatt cgtaccattc acaatatttc
      841 gctgaaccct aactttggcg gcgaagtaat ggtgattggc ctgggttgtg aaaagttgca
      901 gcctgagcgc ctgctgactg gaacggatga tgtgcaagct attccagtag aaagcgccag
      961 cattgtcagt ttgcaggatg aaaagcatgt cggttttcag tccatggtcg aggatatttt
     1021 gcaggtcgcc gaacgccatc tacaaaaact gaatcaacgt cagcgagaaa cctgtccggc
     1081 ttcagaactg gttgtcggca tgcagtgcgg tggcagcgat gccttttctg gcgtaacggc
     1141 aaacccggcg gttgggtatg cgtctgatct actggtgcgc tgcggcgcaa cggtgatgtt
     1201 ctcagaagtc acggaagtgc gtgacgcgat ccatctgctg acaccacgcg cagtgaacga
     1261 agaggtcggc aaacggctgc tggaggagat ggagtggtac gataactatc tcaatatggg
     1321 aaaaaccgac cgcagcgcca acccttcgcc gggcaacaag aaaggcggtc tggcaaacgt
     1381 ggtagagaag gcactcggct ccattgctaa atcgggtaaa agcgcaattg ttgaagtgct
     1441 gtcgcccggt caacgcccga ctaaacgcgg attaatttac gccgcgacgc cagccagcga
     1501 ttttgtctgt ggcacgcaac aggtggcttc gggtatcaca gtgcaagtgt ttacgaccgg
     1561 ccgtggtacg ccgtacggcc tgatggcggt acccgtcatt aaaatggcaa cccgcaccga
     1621 gctggcgaac cgctggtttg atttaatgga tattaacgcg ggcactatcg ccaccggcga
     1681 agaaaccatt gaagaggtgg gctggaagtt gttccacttt attctcgacg tcgccagcgg
     1741 gaagaagaaa accttctcgg atcaatgggg gctgcataac cagctggcag tgtttaaccc
     1801 ggcaccggtg acctgatttc ttttggaacg tccctcgcaa aacatggcct tagtgccatg
     1861 ttttttattg tttaaagccc ccacgtccat taataatgca tttgcattac cttagttcaa
     1921 gcttataatt tgagcagaaa acaggctgta aaaggacagt gaatcatgcc cgctaatgct
     1981 cgctctcacg ctgtactgac cactgaatca aaggtcacga tacgcggaca aacaactatc
     2041 cccgcgccag tgcgtgaggc cttaaaactg aagccaggcc aggacagcat tcattacgaa
     2101 attctgcctg gtgggcaagt atttatgtgc cgactgggag atgaacagga ggatcatact
     2161 atgaatgcat ttttgcgttt tctggatgca gatatccaga acaacccgca aaaaactcgt
     2221 ccattcaaca ttcaacaagg aaagaaactt gtcgctggca tggacgtcaa cattgatgat
     2281 gagattggcg acgacgaata atggattttc cacaaagggt taatggttgg gcgctatatg
     2341 ctcatccctg ttttcaggaa acctacgacg ctttagttgc cgaagtcgag acattaaagg
     2401 gaaaagatcc tgaaaattat cagagaaaag ccgccacaaa gttattggcg gtagtccata
     2461 aagtgattga ggagcatatc acggtcaatc catcatcacc ggcattccgt catggcaagt
     2521 cgttaggctc tgggaaaaat aaagactggt cacgggtaaa atttggtgct ggtcgttatc
     2581 gtctcttctt tcgttatagt gaaaaagaga aagtcatcat tctgggatgg atgaacgatg
     2641 aaaacacgct gcgcacctac ggtaaaaaaa cagatgccta taccgtattc agcaaaatgt
     2701 taaaaagagg acatcctcct gccgactggg aaaccctcac ccgagagaca gaagaaaccc
     2761 attgatggtg ttcacaatgc ccgccgaacg cattcgtgtt gaccagcggg caccctttct
     2821 cactccccga ccagaatcac ttcaacccca gcctttcgca gtccttccag actatccgca
     2881 ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat cttatgtaaa
     2941 ctggaacgat tgaatttact ggaatcggtg accacgatga tccgttccgc aacttcgcac
     3001 atccggcggt ttaaacgggc ttcatcttca ttatgcgtgc tgacgccgcg ctccagatcg
     3061 atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga ttgctcagcc
     3121 tgatcgccgt aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat cagcagctca
     3181 acgccttccg cttccagcaa agcattagcc acgttcatac cgttggtcat cgcaattacg
     3241 tcagtgtgct tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga atcgaggatc
     3301 acccgatgac ctggctgaat caactcaacg gcagctttcg caacgctgcg tttcatcgcg
     3361 gtgttcagcg cgcttttatc ttccactgat ggctcgactg gcggcgtcgt gctatcgcag
     3421 atcaacgcgc caccataggc acgcacagcg atcccctgct tttccagaaa cgccagatcg
     3481 ttgcggatcg tcacagtaga tacgccatac aatgccgaca gatcgttaac ctgcacactc
     3541 ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc tgtcactcgc
     3601 ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact ttcgtttcat
     3661 ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccacctt gcccattgac
     3721 gcaagctact ctcgtttcag tgactttcat tatgtttctt ttgtgaatca gatcagaaaa
     3781 ctattatctt tcgttttatt tttatctcac catgacgcag tatcaactga aacaaaacga
     3841 aagattaata tcgcagtaat ctgaactgga gaggaaagtg aaacatctga cagaaatggt
     3901 gagacagcac aaagcgggca aaacaaatgg aatttatgcc gtttgttccg cacatccgct
     3961 ggtgctggag gctgcaatcc gctacgccag tgcaaaccaa acgccgttac tgattgaagc
     4021 aacctccaat caggtagacc agttcggcgg ttataccgga atgacgcccg ccgattttcg
     4081 cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgt tgattctggg
     4141 tggtgaccat ctggggccaa accgctggca aaacctgccg gccgctcagg caatggccaa
     4201 tgccgatgat ttgattaaaa gctacgtcgc ggcgggattc aaaaaaatcc accttgattg
     4261 cagcatgtcg tgtcaggacg atccgattcc cttaactgat gacatcgtgg ctgaacgcgc
     4321 cgcccgtctg gcgaaagtgg cggaagaaac cagtcttgaa cactttggcg aagccgatct
     4381 ggagtatgtc attggtaccg aagtgccggt acctggcggc gcgcatgaaa ccttaagcga
     4441 gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc gtcacgcctt
     4501 tgaaaagcag ggtctgaatg ccatctggcc acgcatcatt gccctggtgg ttcaacccgg
     4561 cgtcgaattc gatcacacca acgttattga ttatcagccc gccaaagcga ccgccttaag
     4621 ccagatggtc gaaagctacg aaacgctgat tttcgaagcg cactctaccg attatcaaac
     4681 gccgcaatcg ctgcgccagc tggtgattga ccactttgcc attctgaaag ttggcccggc
     4741 gctgaccttc gccctgcgtg aagctctgtt ctctctggca gcgattgaag aagaactggt
     4801 gccagcaaaa gcctgttctg gtctgcgtca ggtgctggaa aacgtgatgc tcgaccgccc
     4861 ggaatactgg caaagccact accacggtga cggcaacgcg cgtcgtctgg cgcgtggtta
     4921 tagctactcg gatcgcgtgc gctattactg gccggacagc cagattgatg acgctttcgc
     4981 tcatctggta cgtaatctgg cggattcacc aattccgctg ccgctgatca gccagtatct
     5041 gccgctgcag tacgtgaaag ttcgctccgg cgagctgcag ccaacgccac gggaactcat
     5101 tatcaaccat attcaggaca tcctggcgca gtaccacaca gcctgtgaag gccaataagc
     5161 aaaacaaaga ggaacacgct atgccaaata ttgttttaag ccggattgat gaacgcttga
     5221 ttcacggtca ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg gtgctggtcg
     5281 ccaacgatga ggttgccgaa gatccggtac aacaaaacct gatggaaatg gtactggcag
     5341 aagggatcgc cgtacgtttc tggacgctgc aaaaagttat cgacaacatt catcgcgccg
     5401 ccgatcgaca gaaaatcctg ctggtttgta aaacacccgc cgatttcctg acgctggtga
     5461 aaggtggcgt tccggtgaat cgcattaacg ttggcaatat gcactacgcc aatggcaagc
     5521 aacaaatcgc caaaacggtt tctgtggatg cgggcgatat cgcagcattt aacgacctga
     5581 aagccgctgg ggtggaatgc ttcgttcagg gcgtcccgac agagcctgct gtggacctct
     5641 ttaaattact ttgagggatt catcatggaa atcagtctgt tgcaggcatt cgcgttgggc
     5701 attatcgcct ttatcgctgg cctggatatg tttaacggct taacccatat gcaccgccca
     5761 gtggtcctcg gcccgttagt cgggctggta cttggcgatc tgcataccgg tattttaacc
     5821 ggcggtacgc tggaactggt gtggatgggg ctggccccgc tggcgggcgc acagccgcct
     5881 aacgtgatta tcggtactat cgtcggcacg gcgtttgcca ttactactgg cgtgaaaccc
     5941 gatgtcgcag tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg gattaccttc
     6001 ctgttctcgg tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga gaacgccgat
     6061 actcgcggca ttgaacgcgt aaactatctg gcactgctgg cactcggcac tttctatttt
     6121 ctctgcgctt tcctgcctat ctacttcggc gcggaacatg caaaaaccat cattgatgtc
     6181 ctgccgcaac gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc agcaatcggt
     6241 tttgccgtgc tgctgaaaat catgatgaaa aacgtctaca tcccctactt catcctgggc
     6301 tttgttgccg cagcctggct caagttaccg gtgctggcta ttgctgccgc cgcgctggcg
     6361 atggcgctga tcgatctgct gcgtaaatct cctgaaccga ctcaacctgc ggcacagaaa
     6421 gaggaatttg aagatggcat ctaatcaaac caccctgccg aacgtcagtg aaaacgaaga
     6481 aacattgctg actggcgtca atgaaaacgt gtatgaagat cagagcattg gcgcggagct
     6541 gacgaaaaaa gatatcaacc gcgtcgcctg gcgttccatg ctgttacaag cgtcttttaa
     6601 ctacgaacgt atgcaggctt ctggctggtt gtacggtctg ctgcctgctc tgaaaaagat
     6661 ccacactaat aaacgcgacc tggcgcgcgc catgaagggg catatgggtt tcttcaatac
     6721 ccatccgttt ctggtgacat ttgttatcgg cattatcctt gcgatggagc gttctaagca
     6781 ggacgttaac agtattcaga gcaccaaaat tgccgtcggt gcgccgctcg gcgggattgg
     6841 cgatgcaatg ttctggctaa cgctactgcc gatttgtggc gggattggag ccagtctggc
     6901 attgcaaggc tccattcttg gcgcagtcgt ctttattgtg ctgttcaacg tggtacacct
     6961 ggggctgcgt tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg cgattccact
     7021 cattaaagca aataccaaaa aagtcggcca tgctgcgtct atcgttggga tgacggtaat
     7081 cggtgcgctg gtggcaacct atgttcgttt aagcaccacg ctggaaatca cagcgggcga
     7141 cgcagtggtt aagttacagg ctgatgttat cgacaaactg atgcctgcct tcttaccgtt
     7201 ggtctacacc ctgaccatgt tttggttggt acgccgcggc tggagtccgc tgcgtctgat
     7261 tgcagttacc gtggttctcg gcatcgtcgg taaattctgc catttccttt aaatacaatg
     7321 aggtattaga tgttaagtat tattttgaca gggcatggcg ggtttgccag tggcatggaa
     7381 aaagcaatga agcaaatcct cggtgagcaa tcgcagttta tcgccatcga ttttccggaa
     7441 acctcaagca ccgcgctgct tacatcgcag cttgaagagg ccatcgccca actggattgt
     7501 gaagacggca ttgtttttct gacggatttg ctgggcggca cgccgtttcg cgtagcttca
     7561 acgctggcga tgcaaaaacc gggctgtgaa gtgatcaccg gcaccaattt acaactgcta
     7621 ctggagatgg tgctggagcg cgaagggtta agcggtgaag aattccgtgt acaggcgctg
     7681 gagtgtggac atcgtgggct gaccagtctg gtggacgagt tagaccgctg ccatgaagaa
     7741 tgtccggtcg aggaagggat atgacacacg ttctgcgcgc cagaaggctg ctgactgaag
     7801 agggatggct cgatgaccat cagttgcgta ttgctgacgg tgtcatcgca gcaatcgaac
     7861 cgattccagt gggcgtgact gaacgcgatg cggaactgct ctgccctgct tacatcgaca
     7921 cccatgtaca cggtggtgcg ggcgttgatg taatggatga cgcgccggat gtactcgaca
     7981 agctggcaat gcacaaggca cgcgaaggtg tcggcagttg gttaccgacc accgtaaccg
     8041 cgccgcttaa taccattcat gcggcgctga aacgtattgc tcaacgttgc caacgcggcg
     8101 gacctggtgc gcaagtgctg gggagttatc tcgaaggacc gtacttcacg ccgcagaata
     8161 aaggcgcgca tccgccggaa ttgtttcgcg agcttgaaat tgccgagctg gatcaattga
     8221 ttgccgtttc tcagcacacc ttacgcgtgg tagcgctggc accggaaaaa gaaggggcat
     8281 tgcaggccat ccgccatctt aaacagcaaa atgtaagggt gatgctgggg catagcgcgg
     8341 cgacctggca acaaacccgc gccgcgtttg atgcaggtgc cgacggcctg gtgcattgct
     8401 ataacgggat gacaggttta catcaccgcg aaccgggaat ggttggcgcg ggattaacag
     8461 acaagcgcgc ctggctggag ctgatagccg atggtcatca tgtacatccg gcggcaatgt
     8521 cgctgtgttg ttgctgtgcg aaagagagaa tcgtactgat caccgacgcg atgcaggcag
     8581 ccgggatgcc ggatggtcgc tatacgttat gtggtgaaga agtgcaaatg cacggtggcg
     8641 ttgtccgtac cgcgtccggt gggctggcgg gcagtacgct gtctgtcgat gcggcagtgc
     8701 gcaacatggt cgagttaacg ggcgtaacgc ctgcggaagc catccatatg gcgtcgctgc
     8761 atccggcacg aatgctgggt attgatgatg ttctgggatc gcttaaaccg ggcaaacgcg
     8821 ccagcgtcgt tgcgctggat agcgggctgc atgtgcaaca aatctggatt cagggtcaat
     8881 tagcttcgtt ttgatggttt gctcctttat tgggccttca cttcccccgt aaggcctttc
     8941 tttttctttc gttttgatct gtgcagcggt gtcggatgcg acgctaacgc gtcttatgcg
     9001 acctacggta cgccgcatcc ggcaatcggt gaacatagcg acgtcattac gcaattcttt
     9061 aatcttccct tgtcttttct ttcaatgatc acagatttcg ttttatttcc tttttctcca
     9121 ttgaactttc ggtttctttt ctatagattt taatcaacga aagatatcac caagtgaaat
     9181 gaaacgaaag gcaagtgaaa gcgacaacgc ccgacgtcaa gttcatcaga ctaaggattg
     9241 agttatgcca gaaaattaca cccctgctgc cgccgcatcc ggtacatgga ctgaagaaga
     9301 gatccgccat cagcctcgcg catggatccg ttcactcacc aacatcgacg cgctacgttc
     9361 cgcgctcaat aacttccttg aaccgttact gcgcaaagag aatctgcgga tcatcctgac
     9421 cggagccgga acctcagcat ttatcggtga catcatcgcg ccgtggctcg ccagccatac
     9481 cggtaaaaac ttcagcgccg taccgaccac cgatctggtc actaatccga tggactacct
     9541 gaacccagcc catccgctgc tgttgatctc cttcggtcga tccggcaaca gcccggaaag
     9601 cgtcgcagcc gtggaactgg caaatcaatt tgtaccggaa tgctatcacc tgccgatcac
     9661 ctgcaacgaa gcgggcgctc tttaccaaaa cgcgatcaac agcgataacg cgtttgctct
     9721 gctgatgccc gcagaaacgc acgatcgcgg ctttgcgatg accagcagca ttaccaccat
     9781 gatggccagc tgcctcgcgg ttttcgcacc tgagacgatc aacagccaaa ccttccgcga
     9841 cgtggcggat cgttgccagg cgatcctgac ctcattgggc gatttcagcg aaggtgtgtt
     9901 tggttacgca ccgtggaaac ggatcgttta tctcggcagc ggtggcttac agggcgcagc
     9961 acgcgagtcg gcgctgaaag tgctggaact gactgcgggt aaactggcgg ccttctatga
    10021 ttccccgacc ggattccgtc atggcccgaa atcactggtc gataacgaaa cgctggtggt
    10081 ggtatttgtc tcaagccacc cttacacccg tcagtatgat cttgatctgc tggctgaact
    10141 ccgccgtgac aaccaggcaa tgcgcgtaat cgccatcgcc gcggaaagca ccgacatcgt
    10201 cgctgccggt ccacatatca tcctaccgcc gtcacgtcac tttatcgacg ttgagcaggc
    10261 attttgcttc ctgatgtacg cccagacgtt tgcactgatg cagtcgctgc acatgggcaa
    10321 tacgccggat accccatcag ccagtggcac cgttaaccgc gtggtgcaag gcgtaatcat
    10381 tcatccgtgg caggcataag aggatcgcat tatgagcatt atctccacta aatatctgtt
    10441 acaggacgcc caggccaatg gctacgcggt gcctgccttt aacattcata acgccgagac
    10501 gatccaggcg atcctcgaag tgtgcagtga aatgcgatcg ccggtgatcc tcgccggaac
    10561 gccggggacc tttaaacata tcgcgctgga agagatctac gccctgtgca gcgcctattc
    10621 cacaacctac aacatgccac tggcgctgca tctcgaccac cacgaatcgc tggatgatat
    10681 tcgccgtaaa gtccacgcag gtgtgcgcag tgcaatgatc gacggcagcc acttcccgtt
    10741 tgccgagaac gtgaagctgg tgaaatcggt tgttgacttc tgccactcac aggattgcag
    10801 cgtggaagca gaactgggcc gcctgggcgg tgttgaagat gacatgagcg ttgacgccga
    10861 aagtgcattc ctgaccgatc cacaagaagc taaacgcttt gtcgaactga ctggcgtcga
    10921 cagcctagcg gtagcgattg gtacggcgca cggcttatac agcaaaacgc cgaagattga
    10981 tttccagcgg ctggcggaaa ttcgtgaagt ggtggatgtt cctctggtgc tacatggtgc
    11041 cagcgatgtt ccggatgaat ttgtccgtcg cactattgaa cttggcgtca caaaagtgaa
    11101 cgttgccaca gaattaaaaa tagccttcgc cggcgcggtt aaagcctggt ttgcggaaaa
    11161 tccgcagggt aatgatcctc gttattatat gcgcgtcgga atggatgcga tgaaagaagt
    11221 tgtcagaaat aaaattaatg tctgtggttc agcgaatcga atttcagcat aatcattagt
    11281 tttttatgat ttatcccaat gtacttcccg attaatatcg gggagtgcct taatggaaaa
    11341 ggagatgact aaaccttaat aaatagatca gtacaatatc gcaacaataa tatatttaaa
    11401 aaaattatat tattcaactt tatggtgagg attacacaat gagcagtcca aatattctct
    11461 taacccgtat tgataaccgt ctggttcacg gtcaggttgg cgtgacctgg acatccacca
    11521 tcggtgcgaa tctgctggta gtcgtggatg atgttgtcgc taacgatgat attcaacaga
    11581 aattaatggg tattaccgca gaaacctacg gctttggcat tcgtttcttt actatcgaaa
    11641 aaaccattaa cgtcatcggc aaagctgcac cgcatcagaa gatcttcctg atttgccgta
    11701 cgccacaaac ggtacgtaaa ttggtagaag gtggcattga cctgaaagat gtcaacgtcg
    11761 gcaatatgca tttctcggaa gggaaaaagc aaatcagcag taaagtttat gtcgatgacc
    11821 aggatctcac ggacttacgt tttattaaac aacgtggcgt gaatgttttc attcaggacg
    11881 tccctggcga tcaaaaagaa caaatccctg actaaatcta aaatcgcctt aatattggtt
    11941 tgaggtaata aaaatgcatg aaataaccct acttcaggga ttatccctgg cggcgttagt
    12001 tttcgttctg gggattgatt tttggctgga agccttattt ttattccgcc cgataatcgt
    12061 ttgtacccta actggcgcta ttctcggtga tattcagact ggcttaatta ccggtggtct
    12121 gacagagttg gctttcgccg gattaacccc tgcaggtggt gttcagccgc ccaacccgat
    12181 tatggcgggt ctgatgacca ccgtcattgc atggtctacg ggcgttgatg ccaaaacggc
    12241 aattggtctt ggcctgccgt ttagtttgtt aatgcagtac gtcattctgt tcttctattc
    12301 tgctttctca ttatttatga ccaaagccga taaatgcgcg aaagaggcgg atacggcagc
    12361 gttttcccga cttaactgga caacgatgct catcgtcgct tcagcgtatg cggtgattgc
    12421 tttcctctgt acttacctgg cacaaggggc gatgcaggcg ctggtgaaag cgatgcccgc
    12481 ctggttgacc cacggctttg aagtggctgg cggtattctg cctgccgttg gttttggctt
    12541 gctgctgcgc gtgatgttca aagcacaata tatcccttac ctgatcgctg gcttcctgtt
    12601 tgtttgctac atccaggtca gcaacctgtt gccggttgcc gtactgggcg caggctttgc
    12661 ggtgtatgag tttttcaatg cgaaatcccg gcagcaagcg caaccgcagc ccgttgccag
    12721 taaaaatgaa gaagaggact acagcaatgg gatctgaaat cagtaaaaaa gatatcaccc
    12781 gtctgggctt tcgttcgtcg ctgctgcaag cgagctttaa ctacgaaagg atgcaagcgg
    12841 gcggttttac ctgggcgatg ttgccgatcc tgaaaaagat ttataaggat gacaaaccgg
    12901 gcctaagcgc agcgatgaaa gataacctcg aatttattaa tacccacccg aatctggtcg
    12961 gattcctgat ggggttatta atttcgatgg aagaaaaagg agaaaaccgc gacaccatta
    13021 aaggcctcaa agtggcactg tttggcccaa tcgccgggat tggcgatgcg attttctggt
    13081 ttactttgtt gccgattatg gcgggaattt gctcatcatt tgccagccag ggaaacctgc
    13141 tggggccgat tctatttttc gccgtttacc tgcttatctt tttcctgcgc gtcggctgga
    13201 cccacgtcgg ttattcagtc ggcgtgaagg cgatcgataa agtgcgagag aactcgcaga
    13261 tgattgctcg ctcggcaacc atcctcggga tcacggtaat cggcgggctg atcgcttcgt
    13321 atgtgcatat taacgtggtg acatcgtttg ccatcgacag tacccacagc gtcgcgctgc
    13381 aacaggattt cttcgataaa gtcttcccga acattttacc gatggcctac accctgctga
    13441 tgtattactt cctgcgggtg aaaaaagcgc atccggtgct gttaatcggc gtgacttttg
    13501 tgctctctat tgtttgttcc gcattcggca ttttgtaaat ggaacgaggc actgcgtctg
    13561 gcggtgcctc tttacttaag gaatttcatc ctgtgcaaac ccttcagcaa gttgaaaact
    13621 atacggcgtt aagtgaacgt gccagcgaat atttattggc cgtgatccgt agcaaaccgg
    13681 atgccgtgat ttgcctggcg accggagcca cgccattact gacgtatcat tatctggtag
    13741 aaaaaatcca ccagcagcag gttgatgtca gccagctcac cttcgtgaag ctcgacgaat
    13801 gggtggatct gccattaacg atgcctagca cctgcgaaac tttcctgcaa cagcatatcg
    13861 tgcagccgct ggggctacgt gaagaccagc tcatcagctt tcgctccgaa gagataaatg
    13921 agacagagtg cgaacgggta acgaacctga ttgcgcgcaa aggcggtctg gatttatgcg
    13981 ttctcggatt ggggaaaaac ggtcatcttg ggctgaacga accgggagaa aacctgcaac
    14041 cggcctgcca tatcagtcaa cttgatgcca gaacacagca acatgagatg ttaaaaaccg
    14101 cgggtcgccc cgtgactcgt gggatcacct taggcctgaa ggatattctc aatgcccgcg
    14161 aggttttgtt actggtgact ggcgaaggaa agcaggatgc gacagaacgt tttctcacgg
    14221 ctaaagtctc tacctctatc ccggcttcgt ttttgtggct gcacagtaac tttatttgtt
    14281 taattaatac ctaaccagat gaaaaatttg ctcaaaatgt ttaaattaac ttattcaaca
    14341 gtcacgcatt atattaaata acatttgact gggttgaaca taacgccaat agcaaaagga
    14401 gttatgttca acagtttaat tttagatata catataactt gttccattaa taaaccatcc
    14461 cgtattctga ctcgccccct ggcgcagcaa agtcatgcac ttatactctt ttg
//
LOCUS       DAZUCE010000125        14427 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZUCE010000125 DAZUCE010000000
VERSION     DAZUCE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.125"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            894..1214
                     /locus_tag="ACHY03_003167"
     CDS             894..1214
                     /locus_tag="ACHY03_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906365.1"
                     /translation="MEARTIPVTLFIHYATSTFSHEKLFIATVDMSKNFPDRYILLES
                     REIEITVNQPQLIDIIGLQVEQLREQKQNTAADAQQRIAAIDDKIQQLLCIEYTPDTD
                     ELPY"
     gene            1245..3458
                     /locus_tag="ACHY03_003168"
     CDS             1245..3458
                     /locus_tag="ACHY03_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906366.1"
                     /translation="MTIFNGLLEAKKGALKNGAIPALAIAIDAPNKKVAENIIIGKLW
                     EAYPDHGDNYFKPKIWEDAPGQPRPGVGEFDETFATEHSFDGEKWVVNTPVVDSGSAD
                     IAQVNDLMKLPARERFAAVLLFSHDANEVDSELLAQTREYLEMLDNSDTDSEDEVDAF
                     NRIVLDAMVACKPIEYMHIAGLNNLVHAILASCDTQEQNPTSWTISKFIKKWVENPGK
                     RDEMLPEVKPETASARPYQQTHATLDREIACALLPVGPEKITPSVLKAADEIISQDRE
                     DFKRWSMALRTTDQILAYDRASVFGVIQSAPAKDTYHFPQSLRSHIDNWLQANGQRDA
                     NAVEEKPKETAPKDDVKVTNHGGGRFSIDGMMSETPSNQGEKSEAANAGERSLQQLRE
                     QFVTPRHVYDVPENNAVSQREPAAITPAEETPPQEKLSEQVKDLVQNVDALVERIHTE
                     EHKRQNAISAIETELKDSGDTDNLALWKNVFKTDERFTKAFSQNGGGTSINGTYIAMK
                     ATREFGPFGIGWGVEVVEERFDKGAPIVRKKQVGEKIEWDLIQDGVGGYLCEMHHTMK
                     VRVWYILNGVRGESEAYGCTPYIYDTKYGPTSDGEAPKKSWTDAVKKALSPLGFSADI
                     FMGLYDNPEYRQRNKAEFDIKNASENAEDATRLRKELDEKLINVANTLAAAVTTNEVN
                     KVFGLIAREVDVHRKAAEAKGDKEYSSYLGSRLRRITDIKTERLAAITAAQEQTA"
     gene            3455..4024
                     /locus_tag="ACHY03_003169"
     CDS             3455..4024
                     /locus_tag="ACHY03_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFS3906367.1"
                     /translation="MSTAIAIAKEYASLIDLLETSDELTPEMIADTLEGMEGELGDKL
                     DAMMVICRNLQGNASTCAEEMSRLATRKTSFEGKEKAIRKHMLTCLQAAGLDKLKTAK
                     NTFTDAQGAIRVIIDSKDKIPDEYEGVSLVDVETVITPNKRAIKEVIESAEAVAAEIL
                     ARGETPPAELLNPVPGAHLERGERSLRVR"
     gene            4024..4206
                     /locus_tag="ACHY03_003170"
     CDS             4024..4206
                     /locus_tag="ACHY03_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906368.1"
                     /translation="MLKLTLKRGDAVHLVLADGTNGIIEARSRCELGLHLPENIKVTR
                     EKSAFPPPELITPNQK"
     gene            4256..4480
                     /locus_tag="ACHY03_003171"
     CDS             4256..4480
                     /locus_tag="ACHY03_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016060749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906369.1"
                     /translation="MLRWQPGATLLSAFDIKIGRLSASVRKQALTESDIARACKAADD
                     AISYIMRKDHEKRSRHNHQRRNGRADGKPT"
     gene            4416..4679
                     /locus_tag="ACHY03_003172"
     CDS             4416..4679
                     /locus_tag="ACHY03_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFS3906370.1"
                     /translation="MRSDHDIITREEMVELTGSPLKSKQCEALRRAGIFFMERADGHP
                     KTTWGHFLNPIKFRNLQEVTTRKEDEPDFGAIFNGRKEKEPSR"
     gene            4648..5664
                     /locus_tag="ACHY03_003173"
     CDS             4648..5664
                     /locus_tag="ACHY03_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3906371.1"
                     /translation="MAGKRKNPADNWMPPRVYQGKAAYEFRNKDNKAIRLCALDAPRS
                     AVWLAYEKAVGDEKERNTFQALAEQFMTSPDFMDLAVETRKDYTKYSGKVLPVFGKID
                     PDKIKPEHIRRYMDQRGLSSRTQANREKSFMSRVFRWGYERGYVQRNPCQGVKQFKET
                     ARERYITDEEYQAVYEVSPDVVRVAMEIAYLCVARQSDVLSLQKDQLFDSGIYIRQGK
                     TGVKQIKAWSPRLQKAIALARSLPLKPGISSLFVIHQTTGGKYTRDGFNSRWRDVKAA
                     AQEKYPHLQIDFTFHDLKAKGISDLEGSLEEKQAISGHKNPRQTAAYDRKVKVVPVVG
                     GQRK"
     gene            complement(5795..5881)
                     /locus_tag="ACHY03_003174"
     tRNA            complement(5795..5881)
                     /locus_tag="ACHY03_003174"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5845..5847),aa:Leu,seq:taa)
     gene            complement(5894..5967)
                     /locus_tag="ACHY03_003175"
     tRNA            complement(5894..5967)
                     /locus_tag="ACHY03_003175"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5933..5935),aa:Cys,seq:gca)
     gene            complement(6022..6097)
                     /locus_tag="ACHY03_003176"
     tRNA            complement(6022..6097)
                     /locus_tag="ACHY03_003176"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6062..6064),aa:Gly,seq:gcc)
     gene            complement(6249..6797)
                     /gene="pgsA"
                     /locus_tag="ACHY03_003177"
     CDS             complement(6249..6797)
                     /gene="pgsA"
                     /locus_tag="ACHY03_003177"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFS3906372.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6854..8686)
                     /gene="uvrC"
                     /locus_tag="ACHY03_003178"
     CDS             complement(6854..8686)
                     /gene="uvrC"
                     /locus_tag="ACHY03_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFS3906373.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDESKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(8683..9339)
                     /gene="uvrY"
                     /locus_tag="ACHY03_003179"
     CDS             complement(8683..9339)
                     /gene="uvrY"
                     /locus_tag="ACHY03_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFS3906374.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            9635..9811
                     /gene="yecU"
                     /locus_tag="ACHY03_003180"
     CDS             9635..9811
                     /gene="yecU"
                     /locus_tag="ACHY03_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFS3906375.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9798..10022
                     /gene="yecF"
                     /locus_tag="ACHY03_003181"
     CDS             9798..10022
                     /gene="yecF"
                     /locus_tag="ACHY03_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFS3906376.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(10090..10812)
                     /gene="sdiA"
                     /locus_tag="ACHY03_003182"
     CDS             complement(10090..10812)
                     /gene="sdiA"
                     /locus_tag="ACHY03_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFS3906377.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMETAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(11042..11794)
                     /gene="tcyN"
                     /locus_tag="ACHY03_003183"
     CDS             complement(11042..11794)
                     /gene="tcyN"
                     /locus_tag="ACHY03_003183"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFS3906378.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPKQPRTRQFLEKFLLQ"
     gene            complement(11791..12459)
                     /gene="tcyL"
                     /locus_tag="ACHY03_003184"
     CDS             complement(11791..12459)
                     /gene="tcyL"
                     /locus_tag="ACHY03_003184"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFS3906379.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(12474..13460)
                     /gene="dcyD"
                     /locus_tag="ACHY03_003185"
     CDS             complement(12474..13460)
                     /gene="dcyD"
                     /locus_tag="ACHY03_003185"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFS3906380.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(13565..14365)
                     /gene="tcyJ"
                     /locus_tag="ACHY03_003186"
     CDS             complement(13565..14365)
                     /gene="tcyJ"
                     /locus_tag="ACHY03_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3906381.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT         3889 a         3715 c         3471 g         3352 t
ORIGIN      
        1 attttttaaa aatacgttga ccattacaaa tacaaattgt aaagtcactt tcatcaacaa
       61 cgcttaccca gcggcagttg ttcagaaaca cgttctgaca gccggaaaga cggcatcaaa
      121 ttttgcgcgt cggcgccaac acgatgacag agggaaagac ttcgccggca tatggcacat
      181 gtgtcgaagc ggtctggatg gaagcggagc cttaacgcgt tgtctccata gcaggtagcc
      241 ggaatgtgca agccacagcc aggtatgagc gattgattca ccatcaaggc gatacggtgt
      301 gaccaccagg gaagagtcct ggctacaaca cgagagcgca cttcatcgac tcaactttga
      361 gctttgtcgt taaattttga aatggcggag tgcgctcccg gttgtggtga acaggtgttt
      421 aacgggaact ccctgcccgt tacccggttc gattccgggc gcccatcatc aatttgctgt
      481 gtttagtctt tgcccagtcc gcacgatggg ccattttttc acacagccag gttttatcgc
      541 tgtgcctgag tccccaacag gagaggccaa acccgcagcg tgacaccagg gaaagaccgg
      601 aggaagtacc acgcctgacc agcgttgacc atgagcctga ccagctcaaa acaggaaaga
      661 ccagccccgt acggtcgtga tggaaacata acgacgccgg aaacgtaacc ggcacccttt
      721 agatagcaaa agacccgcac aaggcgggcc agttaccccg aacggcgacc aaaccattcg
      781 gatttatcac aagtgaccaa acttgtgatg aggaaagacc aacgacactg acgctatgga
      841 agctgatcaa tattcgctga tcggttctga gtatacatca ccaaggagtc gctatggaag
      901 cgcgcaccat cccagtaaca ctctttattc attatgcaac ttcaactttc agccatgaaa
      961 agctgtttat tgcaaccgtt gatatgtcaa aaaattttcc agacaggtac atccttctgg
     1021 aaagccgcga aattgaaatt accgtcaacc agccccagct aatcgacatc atcggtttac
     1081 aggttgagca gctgcgtgag caaaaacaga atacagccgc cgacgctcaa cagcgtattg
     1141 ctgctatcga tgacaaaatc cagcagttac tttgcatcga atacacgcca gataccgatg
     1201 agctccccta ctaaaaacca ctgacctgta atgaggaaag accaatgacc atttttaacg
     1261 gcttgttaga agcgaaaaaa ggcgcgctca aaaacggcgc gatcccggcg ctggccatcg
     1321 ccatcgacgc ccctaacaaa aaagttgccg agaacatcat catcggcaaa ttgtgggaag
     1381 cctaccctga ccacggcgac aactatttca aacctaaaat ctgggaagat gccccgggcc
     1441 aaccgcgccc aggcgttggt gagtttgatg agacgttcgc cacagaacac agttttgatg
     1501 gcgaaaaatg ggtagttaac acccccgttg tagatagcgg ttcagcagat atcgcacagg
     1561 ttaacgacct gatgaaactg ccggctcgtg aacgattcgc cgccgttctg ctgttcagcc
     1621 acgacgccaa cgaagtcgac agtgaattgc ttgcgcagac gcgtgaatac ctggagatgc
     1681 tcgacaacag tgatactgac agtgaggatg aggttgacgc gtttaaccgc atcgttcttg
     1741 atgccatggt ggcgtgtaag cccatcgagt acatgcatat tgctggattg aataatctgg
     1801 tacatgcaat tttggcaagt tgcgataccc aggaacaaaa cccgaccagt tggactatct
     1861 ccaaatttat aaaaaaatgg gtagagaatc ccggtaaacg cgatgaaatg ctgccggagg
     1921 taaaaccaga aacagcatcc gcacgtcctt accaacagac tcacgccaca ctggatcgtg
     1981 aaattgcctg cgccctgtta cccgttggcc cggaaaaaat cacccccagc gtcctgaaag
     2041 cggcagacga aataatcagc caggatcggg aggattttaa acgctggtca atggccttgc
     2101 gcacaacgga tcagatcctt gcctatgacc gcgcatcagt attcggtgtt attcagagcg
     2161 cccccgcaaa agacacgtac cacttcccgc aatccctgcg cagtcacatc gacaactggc
     2221 tgcaagctaa cgggcagcgt gatgcaaatg ctgttgaaga gaagccaaag gaaacagcac
     2281 caaaggatga tgtaaaagtc accaaccacg gcggcggacg attctcaatt gatgggatga
     2341 tgtcagaaac accctcaaat cagggcgaaa aaagcgaagc agcaaatgct ggagaacgta
     2401 gtttgcagca gttgcgtgag cagtttgtca cgcctcgcca tgtgtatgac gtacctgaaa
     2461 ataacgccgt atcacagagg gaacctgcag ctattacccc ggcagaagag actcctccac
     2521 aagaaaaact tagcgagcag gtaaaggatc tggtgcagaa cgttgacgcg ctggttgagc
     2581 ggatccatac cgaagagcac aagcggcaga atgcaatctc agctatcgaa acggagttaa
     2641 aggactccgg cgacacggat aacctggcat tgtggaagaa cgtattcaaa accgatgagc
     2701 gcttcaccaa agctttctct cagaacggcg gcggcacatc catcaacggc acttatatag
     2761 cgatgaaagc gactcgcgaa tttgggccgt ttggtattgg ctggggtgta gaggtggtgg
     2821 aagagcgttt tgataaaggc gcaccgatag ttcgcaaaaa acaggttggt gaaaaaatag
     2881 aatgggatct gattcaagac ggagttggcg ggtacctatg tgaaatgcac cacaccatga
     2941 aagttagagt ctggtacata ttgaatgggg tgcgcggtga gtccgaggct tacggatgca
     3001 caccttatat ctacgacact aaatacggac caacaagcga cggcgaagcg ccgaagaaat
     3061 cctggacgga tgctgttaag aaagcgctat cccctctcgg attcagcgcc gatattttca
     3121 tgggcctgta cgacaatccg gaatatcgcc agaggaataa agctgagttt gatatcaaga
     3181 atgccagcga gaatgcggaa gatgctaccc gtcttcgtaa ggaactggat gagaagctta
     3241 tcaacgttgc caatacgttg gccgccgcgg taaccaccaa cgaagtaaac aaggtatttg
     3301 gcctcatcgc ccgcgaagtg gatgtacacc ggaaagcagc ggaagccaaa ggcgataaag
     3361 aatattcatc gtatctggga tcacgtctgc gccgtatcac cgacattaaa accgaacgcc
     3421 tcgccgccat caccgctgca caggagcaaa cagcatgagc accgctatcg ctattgcaaa
     3481 agaatatgcc agcctgattg acctgctgga aacctccgac gaactgacgc cagagatgat
     3541 cgccgacacg cttgaaggta tggaaggaga actgggagac aaactggacg ccatgatggt
     3601 catctgccgc aatttacagg gcaatgccag tacctgcgct gaagaaatgt ctcgcctggc
     3661 tacccgtaag acgtcatttg aaggcaagga aaaagcaatt cggaagcata tgctcacctg
     3721 cctgcaagct gctggcctgg ataaactgaa aaccgcaaaa aacacattta ctgatgcaca
     3781 gggggctatc cgggtaatta tcgatagcaa agataagatc ccggatgagt atgaaggcgt
     3841 gtcacttgtt gatgtagaaa cggttattac accaaacaag cgagccatta aagaagtgat
     3901 tgaatctgcg gaggcggttg ccgccgaaat actggcgcgt ggagaaacac caccagccga
     3961 gttattaaac ccggtaccag gcgcacatct ggaacgcggc gaacgttcgc tgagggtacg
     4021 ctaatgctta aactcacatt aaagcgtggt gacgcggttc acctggtact tgccgatggt
     4081 accaatggca ttattgaagc acggagtcgc tgcgaacttg ggctacacct gccagaaaac
     4141 attaaggtca cgcgggagaa atcggcattc cccccaccag aactgattac gcctaatcag
     4201 aaataaaaac tcaccatcgc tagcattgcg attcaccagt aaccggagga tcacaatgct
     4261 gcgatggcaa ccgggagcaa ctctgctctc cgcatttgat atcaaaattg gccgactgtc
     4321 ggccagcgtc aggaaacagg ctttgaccga gtctgatatt gcccgtgcct gcaaggcggc
     4381 agatgacgca ataagctaca taatgaggaa agaccatgag aagcgatcac gacataatca
     4441 ccagagaaga aatggtcgag ctgacgggaa gcccacttaa atcaaagcaa tgtgaggctc
     4501 ttcgccgggc tggaattttc ttcatggaaa gggctgacgg gcacccaaaa acaacctggg
     4561 gccatttctt gaacccaata aaatttcgca atttacagga ggtgacgacg cgaaaagaag
     4621 atgaacctga ttttggagca atatttaatg gccggaaaga gaaagaaccc agcagataac
     4681 tggatgccgc ctcgtgttta ccagggcaaa gcggcctacg aattcaggaa taaagataac
     4741 aaagcgatac gcctgtgcgc attggatgcg ccacgatcag ccgtatggct ggcatatgaa
     4801 aaagcggtcg gtgacgaaaa agaaagaaat acttttcagg cgctcgcgga acaattcatg
     4861 acctcccctg attttatgga tttggcagtc gaaaccagga aagactacac aaaatattcc
     4921 ggaaaagttc tgcctgtctt cgggaagatc gacccggata aaatcaaacc tgaacatatt
     4981 cggcgctata tggatcaacg cggtttatca agccgaactc aggcaaacag ggaaaaaagc
     5041 tttatgtcca gggtattccg ttggggttat gagcgaggtt atgtccagag gaatccttgt
     5101 cagggagtta agcagtttaa agagacagca cgcgaacgtt atattacgga tgaagaatat
     5161 caggccgttt atgaagtatc tcctgatgtt gttcgcgtgg caatggagat tgcctactta
     5221 tgcgtggcca gacagagtga tgtactttca ttgcagaaag accagctgtt cgattcaggg
     5281 atctacattc gtcagggaaa aaccggcgtt aagcaaatca aagcctggtc gcctcgtctg
     5341 cagaaagcga tagctctggc tcgttctctg ccattaaaac cgggaatcag tagcctgttt
     5401 gtgattcatc aaactaccgg aggcaagtac acccgtgatg gttttaactc tcgttggcgt
     5461 gatgtcaaag cggcagcaca ggaaaaatat cctcatctgc aaatagactt cacatttcat
     5521 gatctgaaag caaaaggtat ctctgatctg gaaggcagcc tggaagagaa gcaagcaatt
     5581 tccgggcata agaacccacg acagacagca gcatatgacc gaaaagttaa agtagtgccc
     5641 gtagttggtg gccagagaaa atgaatgatg atgcgtccag agaaaaatca tcttcggacg
     5701 catcttcgga aatgaaaaag aagatacaaa aaaaccaccc gaaggtggtt tcacgacact
     5761 gcttattgct ttgattttat tcttatcttt cccatggtac ccggagcggg acttgaaccc
     5821 gcacagcgcg aacgccgagg gattttaaat cccttgtgtc taccgattcc accatccggg
     5881 ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg actagacgga tttgcaatcc
     5941 gctacataac cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca gacatccgct
     6001 tgaggccgat gtcttttaaa ctggagcggg aaacgagact cgaactcgcg accccgacct
     6061 tggcaaggtc gtgctctacc aactgagcta ttcccgcatt catcaagcaa tcagttaatc
     6121 acttgatttt attatcgtct ggcaatcagt gccgccgttc gatgcgttgc attctactta
     6181 cctggcgcga tgagtcaacg atatttttca ccacttttga tcgtttgctg aaaattgcgc
     6241 cgaaacgatc actgatcaag caaatctgca cgcgcagcgc tcaaatattg caacattgac
     6301 cacagagtca gtaccgcagc cacaaagaaa agtgcaatac cggcgtactc aacccaaatg
     6361 ttcggacgcc acagcagcca tgccaacgcc accatctggg cagtggtttt cactttccca
     6421 atccaggaga cagccacgct actgcgttta cccaactccg ccatccattc gcgtagcgca
     6481 gaaataataa tttcacgggc gatcatcgtt gccgccggta aagtcaccca ccagctgtgg
     6541 taatgctcgg ttaccagcac catggcgata gccacgagaa ctttatctgc tacagggtca
     6601 aggaaagcac caaaccgggt actctggttc cagcggcgtg ccagaaaacc atcgaaccag
     6661 tcagtcaccg ccgcgacgca gaaaatgagc gcggcggcaa acggcgacca ggtgacaggc
     6721 aaataaaaga ccaatacaaa gaatgggata aggatgacac ggaacagtgt aagcaacgta
     6781 gggatattaa attgcataat gacgggtaac tatctgttgt cagtaagatt acccctatgt
     6841 tgctacagag acatcaatgt ttcaacgacc agaagatctt ttctgccaga ccttgcgaaa
     6901 tacccggcac ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc aaaccgccca
     6961 tatatttcaa caacatttgc cgacgttttg gcccgacgcc ttcaatggtt tccagggaac
     7021 tggtattttt gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga tcgtgtgatt
     7081 catcgcgaat atgctggata acatgcagtg cgggagaatc tggcggcaaa ctgaatccct
     7141 caccttccgg ctcaaagaac agcgtttcca gcccagcctt acgatctgct cctttggcaa
     7201 cgcctagtag cagcggatga tttttatccc atgagacatc cagttcggcg aagacatttt
     7261 tcgcctgcgc aagttggcct ttgccgccgt cgataaggat cacatccggg atcttactct
     7321 cgtcgatggc tttaccataa cgccgacgca gcacctgatt catcgccgca taatcatcgc
     7381 ccggcgtgat gccagtaatg ttatagcgcc gatactccgc acgcagcggg ccgttagcat
     7441 caaacaccac acaggaagcg acggtttgtt cacccatggt atggctgatg tcaaagcact
     7501 ccatccgctt cacttccggc aatttcaaca cgctggcaag cgcggtcagt cgctggtgaa
     7561 cggtagattg ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg cgcgcgagtt
     7621 tcagataacg cgccctatcg ccgcgaggtt tggtttgaac attaatcttg cgtcccgcca
     7681 gttctgaaag ggaatcggcg agcagcgttt tatcgctaag attaaaatcg agcaggatct
     7741 cacccggtaa ggtgcgcatc tggctgcctt gtaaatagaa ctgacctacg aaggtttcca
     7801 ccacctcgct cagttccgta ccgccaggca ctttcgggaa atagctgcgg ctgccgagca
     7861 ctttgccctg acgaatgaac aatacgtgga cacaagccat gcccgcatcg aacgccacac
     7921 caataacgtc aaggtcgtcg ccggtattgg acacaaactg tttttcggtg acgcgtcgca
     7981 ccgcctgaat ttggtcgcga atacgtgccg cttcttcaaa ctccagattc tggctggcag
     8041 tttccatacg gctaatgagt tgcgtaagca cctgatcatc tttgccagac aaaaacaggc
     8101 gcacatactc gacctgctga gcgtattctt cttcactcac cagtccttca acgcacggtc
     8161 ccagacagcg ccctatctga tattgcagac acggacgcga gcgattgcga taaacactat
     8221 tttcgcactg gcggatgggg aatatctttt gcagtagcgc cagtgtttca cgtacggcat
     8281 agccattcgg gaacgggcca aaatattcac ctttggcatg cttcgcacca cgatgcatcg
     8341 ccagacgcgg gtgggtatcg ccactcagga agataaaggg ataagattta tcatcgcgta
     8401 gcaaaacgtt gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc aacagcgctt
     8461 cggtttctgt atgagtaacc gttacatcaa tttgctggat ctgggcgacc agcgcttcgg
     8521 ttttgcgcga agcgaggttg ctacggaaat agctggaaag ccgttttttc aggtctttcg
     8581 ctttgccgac atagataacc gtaccaccag catcgtacat gcgataaacg cctggctggc
     8641 tggttacggt ttttaaaaac gcttttgcgt caaactgatc actcactgac ttgataatgt
     8701 ctccgcatta cacagaccat ggcgaattgc caggtgagtc agctcaacat cgccatgaat
     8761 gtttagttta ctgaacattc gatagcggta gctgttcacc gttttcggac tgagattgag
     8821 ctgttctgag atctcattga ccttctggcc cttggtgatc atcagcataa tctgcaattc
     8881 acgttcagac aaactggcaa atgggctttc tgttttttct ggttcgatct ggcttaacgc
     8941 catttgttga gcgatgtcag aagcaatgta acgctgccct gaatagacag aacgaatcgc
     9001 actcacgact tcctgcggag ccgcgccttt gctgaggtag cccgcagcac cggcctgcat
     9061 gactttcgct ggtaaagggt tttctgtatg gacggtaagc atgatgattt tgacatcagc
     9121 tgtggaacgc gcgattttac gcgtcgcctc aagaccgcca atgcccggca tactcatgtc
     9181 cattagcacc acgtcaacgg catttgcccg gcaccactta acggcgtctt caccgcacga
     9241 tgcctcaccg acgactttta taccctttat atcttccaga atgcgtcgta tccctgcgcg
     9301 caccagttcg tggtcatcaa caagtagaac gttgatcaaa ggaatatctc cagaaatagg
     9361 gataacgcta ctgatagtta gtcattcgta tattagcggt ttttattgca actttgaaac
     9421 gttaaaaatg gtgggtattc gatttttctc tcgtttttgt aaattcgatt gtccacagtt
     9481 atggtcacgc ccgtcaccaa cggcttccgg gatcgtttca cagtaagccc tgcgatgaaa
     9541 tgtgacaaaa attgacttta ttcagcaaaa atgaaaatca gcccgctgaa tatgtaacat
     9601 taattaaccc gaacaatacg ggcaaaaaac attcatgata aaaatattta ttggtcatta
     9661 tattaacgta ttttatagca ctgccgatat cacgctcaaa aaacaaccac tgctattttt
     9721 agcaaagctt atggtatact ccgccgcctt aacatttttc accgcaaatt ttcattgcaa
     9781 catgacgagg aaaataaatg agtacgcctg atttttctac tgccgagaat aatcaagaac
     9841 tggcaaatga agtctcctgc ctgaaagcga tgctgacgct gatgctgcag gcgatgggac
     9901 aagctgacgc gggccgcgtg atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg
     9961 aaacccaggc tgcagtattt tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt
    10021 aatataaaac cggctgatag cgtgcctttc agccggtttt tgcatctggc acgcaggaca
    10081 gaaaagagat caaattaagc cagtagcggc cgcgtaacag gcaacctggg tcttatttgg
    10141 tgcattaatt tttttctgca tgtttttctg gtggaaattg accgtattct cagagattga
    10201 caaaatcatc gctatctctg ctgatgtctt cccttccgcc gtccacctca gaatttcttt
    10261 ttcgcgcttg ctgaaattca tctctggcgt catcactatt tcatcattta aacgcatcag
    10321 agccatcaga ctttcgcgca ccagtaactg catttttaat tgcagttcat cactaagaat
    10381 gggtatttcg cgcgcgctgc aacgggaaaa ggacaaaaag cccagcgccc ggtttggcag
    10441 cattaaatac tgagtgacac cgcggcgtaa accatgtgcg cgcgcggctt cccataacgg
    10501 ctgtgcttcg ttgaataagt catcattcca cattagatgg ccctgactaa agttttcagg
    10561 gttcagcacc ggatcaattg cgagaaagtt ttttgcctga taataactaa cccacgcctc
    10621 agggtaattg gtgtagaaag ccactttagg tcgagtgaat ggtaccgggt ggcggacaca
    10681 taacgaatag taatcgtact ccagctgctg agcctgaagc tcaatttcat ggtagacctc
    10741 ttctgcggtc tccatcctct gaaaacgcaa cagcatcgtg cgacgccagc tgaaaaaatc
    10801 cgtatcctgc atagtaaacc gcaacgcccc tgagagtgag tatcatttat aatgataatt
    10861 caaactatca cataaataag atttatatat aatttatatt attcaggcaa tgaattactt
    10921 ttgcaagcca tcgcattctc ttatgttatt aatgagttat gctgatttgt taagcagttt
    10981 tatcaggctt gaaatggcgt cccgccccga caggtgaatc gtcggggctg attttttctt
    11041 attattgcag cagaaacttc tcgaggaact ggcgggtgcg aggctgcttg ggatcggcaa
    11101 ataacgcttt tgcggccccc tgctcgacta tccgcccctg gtccataaaa atcgcccggt
    11161 ccgcaacatc ccgggcaaag ctcatttcgt gtgtcacaat caccatcgtg cgcttttcct
    11221 gcgccagctg acggatggtg ttcaggactt cgcccaccag ctctggatcc agcgctgacg
    11281 ttggctcgtc aaacaaaatc acctcaggac gcattgccag cgcacgcgca atcgcaacac
    11341 gctgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg gccagcccaa
    11401 ctttcgccaa cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg cctttgacga
    11461 tcaccggccc ttcaataatg ttctccagca ccgtacgatg cggaaacaaa ttaaagttct
    11521 ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc tgacttaatg
    11581 aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc ggttgttcca
    11641 gcagatttat gctgcgtagc aacgtggttt tgccggaacc actcggaccg ataattgcca
    11701 ccacttcgcc aggctttacc tcaaggtcga taccgtgcag caccgtctga ccgtggaatt
    11761 ttttcaccag gttcttaact tcaatggcac tcattttggt tctctctcct ggcgattaag
    11821 ttgattctca aaatggttct gcaacgtcga taacacagtc gccatgatcc agtagatgag
    11881 cgaagccgcc agatacatgg tgaacacctc cagcgtacgc gaggtaatca actgcgcctg
    11941 acggaacagc tccggcacct ggattgtcgc ggccagcgac gtatctttta ccagactgat
    12001 aaagctgttt gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa tcgcgcgacg
    12061 catggtctgc cacggcgtca taccaatact ggctgccgct tcccactgac ctttatcaat
    12121 agaagaaatg gcggcgcgca gcgtttcggc agcataggcg gcagtgttta acgacagacc
    12181 aatcattgct gacggaatgg gatctaattc gatgccaaac tgcggcaggc cgtaatagat
    12241 cataaacagt tgtgcgatga gcggcgtacc acgaaaaata gagatataaa aacgcgccag
    12301 ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc ctagcagtaa
    12361 gccaaaaaac atgccgccga tactgagttg cagggtatac ccagcccctt tcagcaggaa
    12421 cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgttcccg gagctaaacg
    12481 tggggatgat aggcgaacag cgcaggcgca ccgccggtat gaataaacag aatcggccct
    12541 tcatctttga agcgtttctg actgataccg tcaatcagac ccgccatcgc ttttccggta
    12601 tacacaggat caagcagaat gccttccagc cgcgccagca atttcactgc ttccatgcct
    12661 tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtaa aatttccgct
    12721 gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac tttcggcaat
    12781 tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg catcaggtgt
    12841 tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac caccaccgac
    12901 gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc cacataacct
    12961 agtgcgccaa gagcattaga accgccaacc ggaatgacat acgggcgaaa gccttgtgct
    13021 tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc gtcgcacatt
    13081 tcaatctggg tattgaacag atccagcaac aagcgattgc cgttggttaa atagttttct
    13141 gcggttgtgc caataggatt ttccagcagc gccacacagt gcagtccgag tttcgcagcg
    13201 actgcggcag tctggcgcac atggttagac tggatcgccc cggcagtaat cagcgtatcg
    13261 gcaccttcgc gcagagcatc tgctgcgaga aattccagct tacgtaattt attgccgccc
    13321 attgccatgg gggtgacgtc atcccgtttg atgaaaattt cccgtcctag ataatcagaa
    13381 aagcgcggca gatattcgag cggcgttggc gcgccgataa actccagccg tggaaaacgg
    13441 gttaaattat gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt tcttattatg
    13501 cacgctgaaa acgcgtaaat aaaaaaggcg ctagtgaaag cgcccttttt tgtcattatg
    13561 ctgattattt ggtcacatca gcaccaaacc atttttcgga aagggcttgc aaagtgccgt
    13621 ctttttgcat ttccgcaatt gcatcattca ctgctttcag caggtcttca tttcctttac
    13681 gcagcgccac gccagactcc tgacgggaga atgcttcacc ggttactgcc agcgtatcgt
    13741 tggttttctt caccagatcc agcgccgcca gacgatcaac gaggatcgca tcgatacgcc
    13801 ctacgcgcag atcctgatat ttggtcgggt catcatcata ggtacgcaca tcgacgccct
    13861 gaacattctg ccgcagccac tcttcatagt tggtgcccag accgaccccc acttttttgc
    13921 ctttcagatc atcggctgtt ttaatggtgc cttcgttacc ttttttcacc agcgcctgaa
    13981 taccagaaat ggtgtacggg gttgagaaat cgtatttttt cttgcgctca tcagaaatgg
    14041 tgacctgatt aatcaccaca tcaatacgtt tagagtccag cgacgccagc ataccgtccc
    14101 atttggtcgg ttttagtgac gcctcaacgc caagatgttt tgccagctgt tgggcaaatt
    14161 ccacttcaaa accggttaat ttgccgtcat ccccctgaaa actgaacggc ggataagttc
    14221 cttccagccc taccagtagc gtgccgcgct ctttgacttt attaagcaga ccttcatctg
    14281 caaaactttt aacgctcatg cccgcaacca gcgccacggc catcacaccc atcaatgcct
    14341 gacgtcccag atgtgctaat ttcatattca ccccgaatgt tgttatgtct gtttgcagtg
    14401 tagagccatt gtttgtaaac acaaaaa
//
LOCUS       DAZUCE010000126        14006 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZUCE010000126 DAZUCE010000000
VERSION     DAZUCE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.126"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..207
                     /locus_tag="ACHY03_003187"
     CDS             <1..207
                     /locus_tag="ACHY03_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906382.1"
                     /translation="AKYKMDDQLDQLLVTREIQDEIKSKIQDKIDELSKNLFNTMTET
                     IENNFDDIFRQQSENMSNYYEFVD"
     gene            complement(385..900)
                     /gene="pgpA"
                     /locus_tag="ACHY03_003188"
     CDS             complement(385..900)
                     /gene="pgpA"
                     /locus_tag="ACHY03_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFS3906383.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(878..1855)
                     /gene="thiL"
                     /locus_tag="ACHY03_003189"
     CDS             complement(878..1855)
                     /gene="thiL"
                     /locus_tag="ACHY03_003189"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFS3906384.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLACKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQSLRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1933..2352)
                     /gene="nusB"
                     /locus_tag="ACHY03_003190"
     CDS             complement(1933..2352)
                     /gene="nusB"
                     /locus_tag="ACHY03_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFS3906385.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2372..2842)
                     /gene="ribE"
                     /locus_tag="ACHY03_003191"
     CDS             complement(2372..2842)
                     /gene="ribE"
                     /locus_tag="ACHY03_003191"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFS3906386.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2931..4034)
                     /gene="ribD"
                     /locus_tag="ACHY03_003192"
     CDS             complement(2931..4034)
                     /gene="ribD"
                     /locus_tag="ACHY03_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFS3906387.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(4038..4487)
                     /gene="nrdR"
                     /locus_tag="ACHY03_003193"
     CDS             complement(4038..4487)
                     /gene="nrdR"
                     /locus_tag="ACHY03_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFS3906388.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4638..5177
                     /locus_tag="ACHY03_003194"
     CDS             4638..5177
                     /locus_tag="ACHY03_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFS3906389.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLDFVRIHDIQPVMQ"
     gene            5476..6360
                     /gene="tsx"
                     /locus_tag="ACHY03_003195"
     CDS             5476..6360
                     /gene="tsx"
                     /locus_tag="ACHY03_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFS3906390.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6537..6884)
                     /gene="yajD"
                     /locus_tag="ACHY03_003196"
     CDS             complement(6537..6884)
                     /gene="yajD"
                     /locus_tag="ACHY03_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFS3906391.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(7013..7984)
                     /gene="secF"
                     /locus_tag="ACHY03_003197"
     CDS             complement(7013..7984)
                     /gene="secF"
                     /locus_tag="ACHY03_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFS3906392.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(7995..9842)
                     /gene="secD"
                     /locus_tag="ACHY03_003198"
     CDS             complement(7995..9842)
                     /gene="secD"
                     /locus_tag="ACHY03_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFS3906393.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(9870..10202)
                     /gene="yajC"
                     /locus_tag="ACHY03_003199"
     CDS             complement(9870..10202)
                     /gene="yajC"
                     /locus_tag="ACHY03_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFS3906394.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10225..11352)
                     /gene="tgt"
                     /locus_tag="ACHY03_003200"
     CDS             complement(10225..11352)
                     /gene="tgt"
                     /locus_tag="ACHY03_003200"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFS3906395.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11407..12477)
                     /gene="queA"
                     /locus_tag="ACHY03_003201"
     CDS             complement(11407..12477)
                     /gene="queA"
                     /locus_tag="ACHY03_003201"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFS3906396.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            12570..13151
                     /gene="acpH"
                     /locus_tag="ACHY03_003202"
     CDS             12570..13151
                     /gene="acpH"
                     /locus_tag="ACHY03_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFS3906397.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13172..>14006)
                     /locus_tag="ACHY03_003203"
     CDS             complement(13172..>14006)
                     /locus_tag="ACHY03_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906398.1"
                     /translation="FNVKEFKDDITSMLCEQPPLRTHQISSSGYVSADKLPKLYELNL
                     KDAQRDLKTTPVDIDGVSLKFKTRERIAEEIAKKTAEKTAKKNSEIIAKQNANSIEEK
                     NIEKNKKETNPEDNFVLKFSQEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQC
                     LYNVARDGSPRYAGLLQQIASKCAILQDFDQQLFWRTFSGGVFHRRISVNSEPEKTDV
                     WRFSTQFDFPHINDNGELSRTVLMFEGVAESNFKDIANHSSECFSYSCYAAPLDVNAK
                     K"
BASE COUNT         3485 a         3734 c         3354 g         3433 t
ORIGIN      
        1 gcaaaataca aaatggatga tcaattggac caacttttgg ttacccgtga aattcaagat
       61 gaaataaaat caaagattca agacaaaata gatgaacttt ctaaaaattt attcaacact
      121 atgaccgaaa ccattgagaa taattttgat gatatttttc gtcaacagtc agagaatatg
      181 agcaattatt atgagttcgt cgactgacga tatttcagtg tcattgaatt atgagtgaca
      241 aagatacacc ttgaaatatc gccgcatagt atattcggcg atgtcgggtt ttactttaac
      301 agagtgcccc aaaattgggg cactgttcat ttataaatca aaccgggtgg tctggaatcg
      361 cctacacctc ccggtcaatg gcattcaaag aatacccagc ggccagtgat gaccgataaa
      421 gtacaggatg cctgcggaaa tcaccccggc gacaatatca tcgatcatga tccccatgcc
      481 gccatgtaca ttgcgatcaa accagcggat cggccacggc ttccacatat ccagaatacg
      541 gaaaatcaca aacccggcgg cgacccactg ccagtcattg gtcggcagcg ccatcagggt
      601 gatccacatg ccaataaact catcccagac gatgctaccg tgatcgtgca cacccatgtc
      661 tttcgccgtt tgatgacaaa gatagacgcc gatacagatc cccaacatca ccaccagcga
      721 gtagagctgc cagggcaaaa aggtcatcag ataccagaac ggaatcgccg ccagcgatcc
      781 catcgtgcca ggaacgattg ggcttaatcc acttccgaat ccgacagcaa gcagatgcca
      841 cggattactc atcttcaggc gacttttcgc gacatcttta tggcgtggca aaatggtcat
      901 atcctttcca gtctaacgtg acaggttcgc cgtcacgaat aaaacaaagc ccttcgatat
      961 cggcggtcat ttgcccgata caggtaaacg gtacgcccag atgaccgaga gccacatcca
     1021 gcgcgccacg gttcagttcc ggcaccgtga aacacaactc gtaatcttca ccgccagaga
     1081 gcgcccagcg cagcgcctgt tccggtacaa catggcgaga aagcgcatca gaaaacggca
     1141 gcaatgccag gtcaatacgt gcgccgcagt cgctggcttt cacgatatgc ccgagatcgg
     1201 aaatcagacc gtcagagaga tcgatagctg aatttgccag atcgcgcagt gactgcccct
     1261 gtaaaatacg cggcgatgga cggagatgac gtttgatcaa gtagtccgca tctttagcat
     1321 cggcaacctg caaacggttt tgcaaaatcg ccagcccggc ggcgctatcg cccggtgtac
     1381 cggtcacata gatccagtca cccggtttcg ccccagagcg cgttaaggct cgtcccatcg
     1441 gaacaaagcc gtggataccc aacgtcattg ataatggccc acgcgtggta tcgccgccaa
     1501 tgagttgcat atcgtaataa ttgagaagat caaacaaact gtcgctgaag gactcaagcc
     1561 acgcttcgtc tacgtccggt aaggttaatg ccagcgtcag ccaggccgga tcggccccca
     1621 tcgctgccag atcgcttagg ttcaccgcca gtgctttaca agccagatca gcaggatcga
     1681 tatcagggag gaaatggttg cccgccacca gcgtatcagt gctgatcgcc agggtctgtt
     1741 tctcggggat attgagaaga gcgcaatcgt cgccgatgcc cagttcgaca tcaagacgag
     1801 aacttcttac acggtcaaaa taacgggcaa tcagggagaa ctcgccacat gccatacgtt
     1861 atgcctcagc aggtaaaaaa gaaaaggccg gcgatcgcgg aacagtcttc cgtgaatcta
     1921 ccggcctgga tatcactttt tgttagggcg aatcacaggt gctgctttat cgagtacgcc
     1981 gttgacgaac ttatggctgt cttctgcgcc gaacgatttc gccagttcga tcgcttcgtt
     2041 aatggccact ttgtatggca catcgctacg tttagacagt tcgtacagcg caatgcgcag
     2101 tactgctttt tctacctgtc ccagttcttc cagcaggcgg gacaggtatg gcttcatcag
     2161 tccgtcgagg tatgcggtat tagtcgccac cccggccagc agctcacgga agtacaggac
     2221 gtcaacgtct tttacatcct gttcagccag gaactggtat tcaacatcag cgatgtcgtt
     2281 ctgggacaac tgccaggagt agagcgcctg gacggcacac tcacgagcgc ggcgacgagc
     2341 agcaggtttc acggatttcc ccttactaat ttcaggcctt gatggctttc aatacattaa
     2401 tcatttcaag cgcggtcagt gcagcttctg cacctttgtt gccagctttg gtgccagcac
     2461 gttcgatcgc ttgttcaatg ctttcagtgg tcagaacccc aaaagcaacc ggaatttcgc
     2521 tgtcctgggc aacatgcgcc aggccgttgc ttgcaccacc agcgacatat tcaaaatggg
     2581 cagtgccacc acgaataacc gtacccagcg caatcaccgc gtcgtattta ccggttttag
     2641 ccagtgcacc cgccgccagc ggcagctcat aggcaccagg cacccaaaca acggtaatgt
     2701 tttcatcttt tacctgaccg atacgtttca gtgcatcaat tgcaccttcc agcaggctgt
     2761 cattgataaa gttgttgaaa cgcgcgatgg tgatggcgac gcgagcgtcc ggggtagcaa
     2821 cgttagcttc aataatgttc attttcttcc ttcgggttcg agtatggccc cgcagggggg
     2881 cggattttag cataatattt cgtgcgctgc ttccctttcg agccgggaga tcatgcaccc
     2941 actaaatgca ggcaaacatc cgggcctaca tgacgtatct ctttgaattt aaattggggg
     3001 gcgtcagcta atttctcaag ccctggcagt gtgcataatc cacgggcgtc gctgcctaat
     3061 agtttaggtg cgatatagac aatcagctca tcgactaaac ccgcctgcag caatgcgcca
     3121 gcgagcgttg gccccgcttc cacccagatg ctgttaatct gctgtttacc cagttgcatc
     3181 atcagtacaa ccagatccag atgaccttta tgctctggaa tcagcaaggt acgcaccgtt
     3241 tccggccact cacgagaatc ttcctgcgta cgcgcgaacc aggtttcgcc gggctgctgc
     3301 acaatgtgat gttccggcgt cacgcgattt tggctatcaa tcacaatacg taccggctga
     3361 cggagatttt gttgcggata gagcgcctga gtttgttcat ccagttcaga ccaacgcacc
     3421 gttaaggcag gatcatccgc cagcaccgta gcgctgctgg ttaaaatggc atgactttgt
     3481 gcgcgcagtc gttgtacgtc gcgccgcgcc tgaggcgaag tgatccactg gctttcgccg
     3541 ctcgccatcg ccgtgcggcc atcaagcgat gcgccaagtt ttaactgaat ataaggaaag
     3601 ccggtgcgca tccgcttgag aaagccttta ttcaattgct cggcttcact catcatcagc
     3661 ccgtggctga cgtcaatgcc agcctgttgc aaacggtaaa gtccacgccc agcgacctgc
     3721 gggttcgggt cctgcatcgc agcgaccacg cgcgctacgc cagcggcgat gagtgcgtca
     3781 cagcacggtg gcgtacgacc atgatggcta cagggttcga gtgtgacata agcggtcgca
     3841 cctttggctt tttcacccgc catacgcaac gcgtgtactt cggcatgtgg ttcacccgct
     3901 cgttggtggt aaccttcacc gacaatttcg ccatctttga caatgacgca cccgacattc
     3961 gggttggggt gggtggtaaa acgtcctcgt tgcgccagct ttagcgcccg cgccatgtaa
     4021 tactcgtcct gcacggctta gtcctccagg cgcgcgatct cttcgccaaa ttctttgata
     4081 tcttcgaaac tgcggtagac agaggcaaaa cggatatagg cgactttatc gagctttttc
     4141 aattgctcca tcaccagatt gccaatcatc ttgctcggca cttcgcgctc accggtggcg
     4201 cgcagctgcg atttaatatg attgattgcc atttcgacgt catcggaact caccggacgt
     4261 ttttccagcg cccgcagcat tccgctacgt aatttttctt cattaaacgg ttcacgcacg
     4321 tcgttgcttt ttacaacacg cggcataacc agctccgcca cttcaaaggt ggtgaaacgt
     4381 tcattacaca ccagacactg ccgacggcgg cgtacggatg aaccctcgcc cacgagacga
     4441 gagtcaatta ccttagtgtc cacggcgaaa cagaatgggc aatgcatacg gcgtcctgac
     4501 caggtggtta acagaaatct attttaccct gaactgacga tacaacaaag gcgcaacgct
     4561 tttgagataa aggatcgatg ctttcgctgc ttttaccctc taccattagg ctaattgcga
     4621 cgagaaaggg acgacctatg aatactaacg tttttcgact gctcctgctg ggaagcctgt
     4681 tcagccttag cgcctgtgtg cagcaaagcg aagtgcgaca gatgaaacac agcgtcagca
     4741 cgctgaacca ggagatgacg caactcaatc aagaaacagt caaaatcact cagcaaaaca
     4801 ggctgaatgc aaaatccagc agtggggttt accttctgcc tggagcgaaa acaccggcaa
     4861 gactggaaag ccagatcggt actttacgta tgtcgctggt gaatattacg cctgatacag
     4921 atggcaccac cctgacgcta cgtattcagg gcgagtcgaa tgatcccttg cccgcattca
     4981 gcggaactgt tgaatatggg caaattcagg gaacaataga caactttcag gaaatcaatg
     5041 tgcagaatca attgattaat gcccctgcca gcgtcctcgc ccccagcgat gttgatattc
     5101 cgttacagtt aaaggggatt tctgtggatc agttagactt tgtacgtatc cacgacattc
     5161 aacctgttat gcagtaaacg tattgcgggg caattttgtg cgtcccgcaa catctttccc
     5221 cgtcattttg ttactccgct tacatcaccc agattgatag taaaagtttg caacaagggt
     5281 gaaagtcagt acaatccctg cccgaatgtg tgtaaacgtg aacgcaatcg attacgtaaa
     5341 tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa taggctcctc
     5401 tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa gggattttca
     5461 aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact ggcgctctct
     5521 tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc cgactggtgg
     5581 caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca gatccgcaac
     5641 gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt ctatggttat
     5701 gcggatgcgc cggtattctt cggcggtaac tccgatgcaa aaggtatctg gaaccacggt
     5761 tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac caatactgac
     5821 cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta cgacatgggt
     5881 cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga tatcgacact
     5941 ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca gaactatggc
     6001 gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt tgtgccgatt
     6061 accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga ctggggttcc
     6121 gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac taataactct
     6181 atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc tgtcgtagct
     6241 cgttactggc acgacggtgg gcagtggaac gacgatgcag aactgaactt cggcaacggc
     6301 aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta caacttctga
     6361 ttatgaaaat gccgggattt attcccggca tttcagattg ttaacaacgt gcgctttgtt
     6421 catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa ttcaatatat
     6481 tgctgaaata acgtaggcct gataagcgta gcgcatcagg catttttacg ttttaatcac
     6541 ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc ttcaccgaca
     6601 tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc ttcggtatat
     6661 ttcgaatgct catgatcgtg gcaatagaga caaagcaatt cccagttact gccatcttcc
     6721 gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag gttggaataa
     6781 acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc tttttcgcga
     6841 tagccgcttt ctaaccgcgc atagtttttt gggatgatag ccatgaaaag ttacctggat
     6901 gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc tccatacatc
     6961 aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt gattacggca
     7021 gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg tgttcgcgct
     7081 tcatacccag tttcagagcc aacgcagatg ccacatagat ggaagatgca gtaccgatgg
     7141 aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg aagagataca
     7201 gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc agcgtctggg
     7261 tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg aagttttcac
     7321 gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc gacatcaacg
     7381 atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc agcgtaatga
     7441 taacgtcgtg cgccagcgca ataaccaccc ctgccgccag tcgccactca aagcggaaac
     7501 ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg gtttgcgcaa
     7561 ggtctgcccc cacgctcgga ccgacgaact caatacgctt cactgctgca ttctgattgg
     7621 tggattcgtt aatcaccttc agaacctggc tgcccagcac ctgaccgccg gtttcgcctt
     7681 cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc agcattggct
     7741 cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc ggtttttcga
     7801 gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag ccgcgcacgc
     7861 ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc cagtagtccc
     7921 agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta tattcctgtg
     7981 ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata tagcaggttt
     8041 acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc gataccggta
     8101 gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat gactttaatc
     8161 agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc ttcatcaatt
     8221 gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt gatcagtacg
     8281 ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg catactcagt
     8341 gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc aatcagagca
     8401 ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat agaaaccagc
     8461 agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag ggtcgggcca
     8521 attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg cagcagcagt
     8581 gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct gttacccaga
     8641 cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac cgcacgacca
     8701 tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat cggtttgccg
     8761 atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag cgagatgtta
     8821 acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg gtcaccggtc
     8881 agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt cacttcagag
     8941 tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cgttggtgtt taccagacgg
     9001 aattccagcg ttgccgtcgc acccagaatc tctttcgcac gcgcagtgtc ctgaatacct
     9061 ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc cgccacgcca
     9121 agttggttta cacggttacg caggatattg atgttctgct gcaccgcata ttcacgcgct
     9181 tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg gctgctaatc
     9241 accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc tttagcatcg
     9301 cggaaagtaa tgctcaggcc gtagttgttt tctttacgaa cggtggtata cgggatgcct
     9361 ttttcgcgca ggtcgctgcg caggctatcg atattttgtt cctggagttt gccaagcgcg
     9421 gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag gccgagcttc
     9481 atcggctcag cgtgaatagc tgccagccag cgcggcgttg ccggggcaag gttaagcgcc
     9541 acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcag ctgggtgtca
     9601 gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt agcagttatt
     9661 ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc ggcgactccg
     9721 cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag cgcatacagc
     9781 agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata acggtttaac
     9841 acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc tttcggcagg
     9901 acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag cgcgatagca
     9961 atgtagccgt tttccgctac tttggttaca cgaccgacca ggccaccgtt cgtcagaact
    10021 tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttagtgcg cttctgctgt
    10081 ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa aatcaaagac
    10141 atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat aaaaaagctc
    10201 attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt ctcgcccctg
    10261 acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag ccttgcgtaa
    10321 acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc gcgcgcctaa
    10381 tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc gacaggtgta
    10441 gcaatcacac tcaggatcga gtgggccagt atcgctctta tacttcgcat tgcggatttt
    10501 caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg gcattacgca
    10561 gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttctggtt taccaacgcc
    10621 catcaggtaa cgcggtttgt ctgccggaat ttgcgggcat acatgctcca gaatgcggtg
    10681 catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat caaaaccgat
    10741 atctaccaga cctttaacag aaatatcacg taaatcttcg taaacgctgc cctgaatgat
    10801 accaaacagc gcatttttgt ttccgagact gtcaaaacgc tcacggctac gcttcgccca
    10861 acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat acggcgtaca
    10921 ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca tcgacttttc
    10981 cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc cctgttcggt
    11041 gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg tgaggatcgg
    11101 tcccttccac tgcataaaat cgtgcagatc gccgtgcaat ttcatgattt cctggcccgg
    11161 gcgcagccac aggtggaagg tgttgccgag gataatttgt gcgccagtgg cttcaacttc
    11221 ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac aaggcgtttc
    11281 cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg tggtgtccag
    11341 ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta aaccagcgcc
    11401 gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga taaacatcgc
    11461 atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg cattcatggt
    11521 gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg gcaagtggaa
    11581 gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa agatttgggt
    11641 atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc tttccagcga
    11701 acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg ccgccagtac
    11761 cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt gatcttcaat
    11821 ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca caaacgccat
    11881 ctccacgcct ttggcgcgta atttttccag caaaggttcg tcaaaatgca gaccagcggt
    11941 cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt cgcggtcagc
    12001 gtcttcgtcc ggacggtcga tatacggcgg cagcggcata tggccgatgc tgttgagaat
    12061 atccagcacc gaacgatcat cattaaattc gacttcaaac agtgcgccgt ggcgcgcggt
    12121 cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag gttttggcgc
    12181 tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct caaccagcac
    12241 ttcaatcttg ccgccgctgg ctttacgccc aaacaggcgc gccgggatca cgcgggtatt
    12301 attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga aagtaccgtg
    12361 cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc gttcaggcat
    12421 cggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa cgcgcatgac
    12481 actgactcag actaaaataa gaggcgggta gtctagtgcc ggggcgctct ccctgcaacc
    12541 tttacccccc tttggataga atataatcga tgaatttttt agcacacctg catttagccc
    12601 atctcgcgga aagctcgctt tccggcaatt tgctggctga tttcgtacgc ggaaatcctg
    12661 aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt atcgacgtat
    12721 tgactgacaa tctgccggaa gtccgcgaag cacgggagtg gtttcgtagt gaaactcgcc
    12781 gcgttgcgcc tattacgctg gatgtcatgt gggatcactt tctttcccgt cactggtcgc
    12841 agctgtcgcc ggactttccg ctacaggaat ttgtttgtta tgcccgcgag caagtgatga
    12901 caattttgcc ggactcaccg ccacgcttta tcaacctgaa caactactta tggtcagagc
    12961 agtggctggt gcgctatcgc gatatggatt tcatccagaa cgtgttaaac ggcatggcaa
    13021 gccgtcgccc acgtctggat gccctgcgtg actcctggta cgatttagac gcccattatg
    13081 ccgccctcga aacccgcttc tggcagtttt atccacggat gatggcgcag gcgtcacgca
    13141 aggcgctctg aaacttcaag ttccggcgtc actatttttt agcattcaca tccagcggag
    13201 cggcataaca actgtaagaa aaacattcgc tggaatgatt ggcgatatct ttaaagttgc
    13261 tttcagcaac cccctcgaac atgagtaccg ttctggacaa ctctccgttg tcatttatat
    13321 gtggaaaatc aaattgtgta ctgaatcgcc atacatctgt cttttctggc tcgctgttca
    13381 cagagatacg tcgatggaag actcctcctg aaaacgtgcg ccagaacaat tgttgatcaa
    13441 aatcttgtaa tatcgcacac ttggatgcta tttgttgtag taaacctgca taccgtggac
    13501 tgccatcacg agcaacatta tacaaacact gactaaaagc atcgaacaaa caatagatgt
    13561 gatcactagt gaaaaaaggt cttaattttt tttgttcttc gtcgctgtaa tccaggagca
    13621 tctcattgat ttcttcctgt gagaatttta agacaaaatt atcttcagga tttgtttctt
    13681 tcttgttttt ttcaatattc ttctcctcaa tgctattcgc attttgtttc gcaattattt
    13741 ccgaattttt tttcgcagtt ttttccgcag tttttttcgc aatttcttct gcaattcttt
    13801 cccgagtttt aaattttaaa ctgacaccat cgatgtcaac cggtgttgtt ttaagatctc
    13861 tctgcgcgtc tttcaaattt aactcataga gtttgggcag tttatctgca gatacgtatc
    13921 cggatgaaga aatctggtgt gtacgcaggg gcggctgttc acaaagcatc gaggttatat
    13981 cgtctttaaa ctcttttaca ttaaac
//
LOCUS       DAZUCE010000127        13759 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZUCE010000127 DAZUCE010000000
VERSION     DAZUCE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.127"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            318..1007
                     /gene="paoA"
                     /locus_tag="ACHY03_003204"
     CDS             318..1007
                     /gene="paoA"
                     /locus_tag="ACHY03_003204"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFS3906399.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGLPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSPPERTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            1004..1960
                     /gene="paoB"
                     /locus_tag="ACHY03_003205"
     CDS             1004..1960
                     /gene="paoB"
                     /locus_tag="ACHY03_003205"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFS3906400.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            1957..4155
                     /gene="paoC"
                     /locus_tag="ACHY03_003206"
     CDS             1957..4155
                     /gene="paoC"
                     /locus_tag="ACHY03_003206"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFS3906401.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPYDRIDGPLKTTGTARYAYEWHEE
                     SPNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVSQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVSVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAL
                     VHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACVKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            4165..5121
                     /gene="paoD"
                     /locus_tag="ACHY03_003207"
     CDS             4165..5121
                     /gene="paoD"
                     /locus_tag="ACHY03_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFS3906402.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSKEETAQ
                     IRAPVGIFPKARNAHTLALSVLAEVASVRLHQDEDSCLPPSS"
     gene            complement(5302..6429)
                     /locus_tag="ACHY03_003208"
     CDS             complement(5302..6429)
                     /locus_tag="ACHY03_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3906403.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPIARDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLGMTVLMAVSGLIIALATSYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPMA
                     VVAFIWQCISLPSMDANKSHTSCGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSTFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(6571..7629)
                     /locus_tag="ACHY03_003209"
     CDS             complement(6571..7629)
                     /locus_tag="ACHY03_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3906404.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGKDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVSAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKAVGSKDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            7894..8778
                     /locus_tag="ACHY03_003210"
     CDS             7894..8778
                     /locus_tag="ACHY03_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3906405.1"
                     /translation="MAKRENYNELYLFMQVVQEGSFTAAAKRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
     gene            9021..9077
                     /locus_tag="ACHY03_003211"
     CDS             9021..9077
                     /locus_tag="ACHY03_003211"
                     /inference="COORDINATES: protein motif:HMM:NF041474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906406.1"
                     /translation="MVVFVATVEYAYLSLHFY"
     gene            complement(9481..9756)
                     /locus_tag="ACHY03_003212"
     CDS             complement(9481..9756)
                     /locus_tag="ACHY03_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906407.1"
                     /translation="MPMYIKLILSIVRRINMNTICSLIVVVALSLLSFSSLAKTITAV
                     GSTINSTEKNFFTGRKQGKSYKILGAFFKNRFYMIAKLTPVSKNDAS"
     gene            complement(9920..10642)
                     /locus_tag="ACHY03_003213"
     CDS             complement(9920..10642)
                     /locus_tag="ACHY03_003213"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3906408.1"
                     /translation="MDKVILITGASSGIGEGIARELGTTGAKTLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQVALDSWGRVDVLINNAGVMPLSPLAAGKQNE
                     WELTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
     gene            10741..11640
                     /locus_tag="ACHY03_003214"
     CDS             10741..11640
                     /locus_tag="ACHY03_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3906409.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGLLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     RQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPEVAPYRWPFEENGRAFYLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            11669..11880
                     /locus_tag="ACHY03_003215"
                     /pseudo
     CDS             11669..11880
                     /locus_tag="ACHY03_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144690.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(12045..12965)
                     /locus_tag="ACHY03_003216"
     CDS             complement(12045..12965)
                     /locus_tag="ACHY03_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFS3906410.1"
                     /translation="MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPR
                     LSVDIDLAYKSFADRDTDLAAINDALKRITASLNSKPGITAIRQENKADEKRIIVNTA
                     DAKIKIEVSPVWRGLLLPPAEMSVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
                     PRDLFDVLNMLEKPGLMREIFDGFICYLAGHPRPIAELLAPNWDTDRIATLYAQEFSG
                     MTQQETSLESLLSVTTLLPQALKSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAI
                     RWKQRNLAVLKDKNPAKYVAAVNKLERVLE"
     gene            complement(12952..13734)
                     /locus_tag="ACHY03_003217"
     CDS             complement(12952..13734)
                     /locus_tag="ACHY03_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFS3906411.1"
                     /translation="MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVQNGWLRR
                     IAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLTSLALQGHAHYTEPGRPQVWLAL
                     PAYVKLPGWFAAFEQFATFITLYTSGLTVDVTSFTTELKIGDRQLAGSALELAAYEIA
                     CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSIKTNRVMLFLARRNGHPYFQYL
                     DQSGIETGTGKRQIIPGGWLEPDYQITVPRAFKPEGVEDGSA"
BASE COUNT         3362 a         3740 c         3550 g         3107 t
ORIGIN      
        1 gcctaatcgc caatgaatat aagcaccatc atttctccct atttttatat tagaagtgac
       61 atagatttgc agggtgatgc agagctgaca atcacaggtt tccttatcgg tttttgcatc
      121 gtacaactaa agcaataaac cagctcccat catcatcaac atcccggcga acatgaattt
      181 attagccggg ctcatagctg cattcgcgtt ttaagaataa tcctcctgct gtcgccgact
      241 atgcttaacg tttaaaaaag catcagcact ctcgcaacgc actcttattt tcccctttag
      301 aataccggag gcctggtatg agcaaccaag gcgaataccc cgaagataat cgggttggga
      361 agcatgagcc gcacgattta agtttgaccc gtcgcgatct gattaaagtg agcgccgcaa
      421 cggcggcggc cgccgtggtt tatcctcatt ctacgcttgc ggcaagcgtt ccggcagcta
      481 cacccgcgcc tgagataatg cccctgacac ttaaggtgaa cggcaaaacc gagcagcttg
      541 aggtggatac ccgaaccacg ctactggacg ctttgcgtga aaatctgcat ttgatcggta
      601 ccaagaaagg ttgcgatcac ggacagtgcg gagcctgtac cgtgctggtc aatggtcgca
      661 ggcttaatgc ctgcctgacg cttgcagtca tgcatcaggg ggccgagatc accaccattg
      721 aaggcctggg tttaccagat aatcttcacc ccatgcaggc ggcctttatc aagcatgatg
      781 gcttccagtg cggctactgc acctccgggc aaatttgctc atcagtagcg gtgctaaaag
      841 agattcagga cggcattccc agtcacgtca cggtcgattt ggtttcccct ccagaaagaa
      901 ctgccgatga gatccgtgaa cgtatgagcg gcaacatctg tcgctgtggt gcatacgcta
      961 acatccttgc cgccattgaa gacgctgcgg gggagataaa atcatgaagg cgtttaccta
     1021 tgaacgagtg aacaccccag ccgaggcggc acttagcgct cagcgcgtac ccggcgcaaa
     1081 atttatcgcg ggcgggacca atctgctgga cctgatgaag ctggaaattg aaacgcccac
     1141 ccaccttatc gatgtgaacg gcctcgggct cgataagatt gaagtgaccg acgcgggtgg
     1201 gctgcgcatc ggcgcactgg tacggaacac cgacctggcg gctcacgagc gcgtgcgtcg
     1261 tgattacgcg gtactctccc gcgccctgct cgctggcgcg tctggtcagt tacgcaatca
     1321 ggcaaccacc gcaggtaatc tgctccagcg cacgcgctgc ccctattttt acgacaccaa
     1381 tcagccctgc aataagcgcc tgcccgggag cggctgcgcg gcgcttgaag gctttagccg
     1441 tcagcacgcg gtggtaggcg taagcgaagc ctgcattgcc acccatccga gcgatatggc
     1501 ggtcgcaatg cggttgctgg atgcggtggt ggaaaccatc acgccggagg gaaagactcg
     1561 cagtatcaca ctggctgatt tttatcaccc accggggaaa acgccgcaca ttgaaaccgc
     1621 cctgcttccc ggtgagctta tcgttgcggt gacgttacct ccgccgctcg gcggaaaaca
     1681 tatctaccgt aaggtgcgcg atcgcgcctc ctacgccttt gccctggtat cggtcgcggc
     1741 gattattcag cctgacggca gcgggcgcgt cgcgctgggc ggagtagcac ataagccctg
     1801 gcgcattgag gctgcggatg ctcagctatc ccagggggcg caggccgtat atgacgcgct
     1861 gttcgccagc gcccatccca ccgctgaaaa cacctttaaa ctcctgttgg cgaagcgaac
     1921 gcttgcctcc gtactggctg aagcgagggc acaagcatga aatttgataa acccgcaggg
     1981 gaaaacccga tcgatcagct gaaggttgtc ggtcgtccct atgaccgcat cgacggaccg
     2041 ctgaaaacta ccggcacggc acgctacgcc tacgaatggc atgaagaatc ccccaacgcc
     2101 gcctatggct atatcgtcgg ttccgccatt gccaaaggac gcctcaccgc ccttgatacg
     2161 gacgccgcgc aaaaagcgcc gggcgtactg gctgtcatta ccgccagtaa cgccggggca
     2221 ctcggcaaag gcgacaaaaa caccgccagg ctgttaggcg gccccactat tgagcactat
     2281 catcaggcca ttgcgctggt agtggccgag accttcgaac aggcacgagc agcggcctcg
     2341 ctggtgcagg cgcactatcg ccgtaataaa ggagcttact ccctggcgga cgaaaaacag
     2401 gccgtcagtc agccgccgga agacacgccc gacaaaaacg tcggtgactt tgacggggca
     2461 ttcacctccg ctgcggtgaa gattgatgct acctacacga ccccggacca gagccatatg
     2521 gcgatggagc cgcatgcctc gatggccgtc tgggatggaa ataagcttac tctctggacc
     2581 tcaaatcaga tgattgactg gtgccgcacc gatctggcaa aaacgctaaa agtgtccgtg
     2641 gagaatgtgc gtattatctc cccgtatatc ggcggagggt ttggcggcaa gctcttcctg
     2701 agaagcgatg cgctgctggc agccctcgcc gcccgagcgg tgaaacgtcc ggttaaagtg
     2761 atgctccccc gccccactat tcccaataac accacgcacc gccccgccac ccttcagcac
     2821 ttgcgtatcg gtgccgacca gagcgggaaa atcaccgcta tctcacatga aagctggtcc
     2881 ggaaacctgc ccggcggcac gccggaaacg gcggtacagc aaagcgaatt actctacgcc
     2941 ggggcaaacc gtcataccgg cctgcggctc gccacgcttg atttgccgga agggaacgcc
     3001 atgcgtgcgc ccggcgaagc ccccggtctg atggcgctcg aaatcgcgat cgacgaactg
     3061 gcggaaaaag cgggcatcga tcccgtcgag tttcgcatcc tgaatgacac tcaggttgac
     3121 cccgccgacc cgacgcgccg cttctctcgc cgtcagctta tcgagtgctt gcgcaccgga
     3181 gcggataaat ttggctggaa gcagcgcaac gccacacccg gacaggtacg cgacggagag
     3241 tggctagtcg gccacggcgt cgcggcgggc tttcgcaata atctgctgga aaaatcgggg
     3301 gctctggttc acctcgaacc aaacggcacc gttaccgtgg aaacggacat gaccgacatt
     3361 ggcaccggca gctacaccat tctggcccag acggcagcgg aaatgcttgg cgtaccgctg
     3421 gagcaggttg cggttcacct cggcgattcc agtttcccgg tttctgcggg ttctggcgga
     3481 caatggggcg cgaatacctc cacctccggc gtttacgccg cctgtgtgaa gcttcgcgaa
     3541 atgattgcct cggcagtcgg gtttgatcct gagcagtcgc agtttgccga cggcaagatt
     3601 accaacggta cccgaagcgc cacgctacat gaggccaccg caggcggcag actgacagcg
     3661 gaagagagta ttgaattcgg aacactgagc aaggagtacc agcagtcgac ctttgccggg
     3721 cattttgtgg aggtcggcgt gcatagcgcg acgggagaag ttcgggtccg gcgtatgctc
     3781 gctgtgtgtg ctgcaggacg cattctgaat ccgaaaactg cacgcagcca ggtcattggc
     3841 gcaatgacta tgggcatggg cgcggcactg atggaggagc tggcggtgga tgaccgtttg
     3901 ggctacttcg ttaatcacga tatggcgggg tatgaggtgc cggtccatgc ggatatccca
     3961 aaacaggaag tgattttcct ggatgatacc gaccccatat cctccccgat gaaggccaaa
     4021 ggtgtcggtg agctaggcct gtgcggcgtg agcgcggcta tcgccaacgc ggtgtataac
     4081 gccaccggta ttcgggtacg ggattatccc atcactctgg ataagctgct cgataagctg
     4141 ccggatgtgg tttaaggagg aacaatgtca tacccgcttt ttgataaaga cgaacactgg
     4201 cataagccag agcaggcgtt tctcaccgat gaccaccgga ccattctgcg cttcgccgta
     4261 gaggcgctaa tgtccggtaa aggagcggtg ctggtgacgc tggtggagat acgcggcggc
     4321 gcggcgcgcc cgctcggggc gcagatggtg gtgcgcgaag atggtcgtta ctgcggtttt
     4381 gtctctggcg gctgcgtgga ggccgctgcc gcttttgaag cgctggagat gatgggctca
     4441 ggccgcgatc gcgaaattcg ctatggcgaa ggttcgccgt ggtttgacat cgttctgccc
     4501 tgcggcggtg ggatcacgct gacgctccat aaactacgct cggcacagcc tctgctcgcc
     4561 gtgctgaacc gcctggaaca gagaaaaccg gcgggtctgc gctacgatcc gcaagcacaa
     4621 tcgctggtgt gcctgcccac gcaaacccga acgggctgga atctcaatgg ctttgaggtg
     4681 gggttcaggc catgcgtcag gctgatgatt tacggacgtt ctcttgaggc gcaggcaacc
     4741 gcgagtcttg cagcagccac aggctatgac agccatatct tcgatctttt tccggcctca
     4801 gccagcgctc agatcgatac cgatacggcg gtcattttgc tgtgccatga tctcaaccgg
     4861 gagctgccag tgttgcaggc cgcgcgagaa gcaaaaccct tttatctcgg cgcattgggc
     4921 agctatcgaa cccacacgtt acgtctgcaa aagctccacg agctgggatg gtccaaggag
     4981 gagacagcgc aaatccgggc acccgtcggg atattcccca aagcccggaa tgcgcataca
     5041 ctggccctct ccgtgctggc agaagtcgcc tctgtacgtc tccatcagga tgaggattca
     5101 tgcctgcccc cgtcgtcctg atccttgcgg ccgggcgtgg agagcgcttt ctcgcctccg
     5161 ggggaaatac ccataaatta agagaaaaaa tctgcagtag ccgttttaga aaatcaccta
     5221 aatcgtttaa aagagcgccc aacccatagt gggcgctcat cgccttgggt aacttattca
     5281 gcaacgcggg ccctcgccgg actaaagcgc accggccttc tggcgtgcgg taacaaaggt
     5341 cagtacgccc gcgcaaagga gcagcacact actcatggca aacgtactct gccagccaag
     5401 gcggtcaaac acgatgcctc ctgccgttga tccaagggcg attgagagct gaatcaccgc
     5461 gaccatgagc ccgcctccgg cttcggcgtt gtcaggcaac gtacgcgcaa tccatgtcca
     5521 ccacccggtt ggcgcggcgg ttgccagcat accccagagt cctaacagca gcgagaccgt
     5581 ccagacatga tgcccggtaa gcatcagacc aatggcaatg acggccatca gtccgggtat
     5641 ggccatcagc gtggggtaga attttctgtt gagaaatgtc gaaacaacca gcgtgccaat
     5701 aaaaccggcc acgccgataa tcagcaaaat caacgacaag cccaaggaat taacccgtgt
     5761 caccgactcc agaaacgggc gcacataggt gaatagcgca aactgcccca tgaaaaacag
     5821 gccacaagct atcatgccga ccacaaccat gcggcggcta aataagcgga ataccgcgcc
     5881 acatgacgtg tggcttttat tcgcatccat gctgggcaga ctaatgcact gccagataaa
     5941 ggcgacaacc gccatgggca ccaagcacaa gaaagcgcct cgccatccga ccgtagcccc
     6001 cagatagctt cccagcggcg cggccacgac cgtcgccagc gcattaccgg cgttgaaaat
     6061 cgccagcgca cgcgtgacct gatgctgcgg caccagacga atcgccgttg ccgctgacat
     6121 cgaccagaat ccgccaatgg ccacaccaat catcgcacga ccgaccatat acatcagata
     6181 actggtagcc agcgcaataa taagccccga tacggccatc agaaccgtca tccccagcag
     6241 caggaactta cgattcatct ttccagctag cgttgaaagc gtcaggctgg tcagaaccgc
     6301 cagcgcaccg gagatagcaa tcccctgccc tgccagcccc tccgtcacgc ccaggtcacg
     6361 ggcaatcggc gtgagcaggc tgacgggcat aaattcagaa gcaatcagca caaacacgca
     6421 cagggtcatg gcaaaaatac cgccccagta tgcgtgctga tgatgaggtg atggtgtttg
     6481 gcttagtgtc gacatagtga aatttcacaa agatcaggat aaaagccacc ggccatagac
     6541 cggtggcttt gcatcaattc aggggaggtt ttatttcagt ttggtctgga aaaattgttc
     6601 gaacttcgcg aaaggtattt ttccggccac gttatcgtac aggtccacgt gatttgcccc
     6661 tggtaccacc accaactctt tgtctttact gccaaccgcc ttgtaagcat cttcagcaaa
     6721 atagcgtgaa tgtgctttct caccggtcac aatcagcgtc ggaatggtga tttcactggc
     6781 gtagctcaac agcggcatat tcataaatga cagcggcatc gttgccgtcc acgccccggt
     6841 agagttaacg gaacgttcgt ggaagccgcg cggcatgcga tagtagtcaa agaactcttt
     6901 cagtaccgga tgaggatttg ccggcagcgt ttctggcaga atgcgctcgc ccgcactgac
     6961 cttgccgttg ctatcgacat taatatcatg accaccgtga gcgaacgttc cgctctgcgc
     7021 atccttccag cgctgttcat tcagatattg cagaacggca cgacggtcgg cggtagaata
     7081 acggtctttg ccatccccca caccatgacc catcgcccgg ctcatgtcat acatcacgct
     7141 ggtagctacc gctttaacgc gggtatccat cgccgcgtcg ttcagcgcca tgccgcccca
     7201 gccacaaata ccgagcagcc cgatacggtt gcgatctacc tctttttgca gccctaagaa
     7261 atccaccgcc gcgctgaaat cttcagtgtt gatatccggt gaggcgacgt ttcgcggata
     7321 gccgccgctt tccccggtgt aagaaggatc gaacgccagg gtaacaaacc ctcgttccgc
     7381 cagcgtctgc gcatacaagc cgctggattg ctctttcacc gcgccaaatg gcccactgac
     7441 cgcaatcgcc gccagcttgc gatcgccacg gtctttaggc aggtaaagat cgcccactaa
     7501 ggtgatgcca tatcggttcg ggaacgatac tttacgatga tcgactttct ggctctcggc
     7561 gaacgtttta tcccatttat ctgtcatgga cacaggggca ttaggattgg ttgaatcagc
     7621 ataactcatt gtcgtgactc cacttaatga tgcgaaaagc agcatggccg gcatcgcggt
     7681 ttttagcgtt cgggtgaagg ctttcataaa aatccccata aaaaacaaca gagagataca
     7741 ggtttccgac ggacaacgct gcggtgcttt actttgatgg ggtcattata gtgagcagaa
     7801 ttagtgctga ttagggggcg attaatgcta agattaatac catattgtta taaattaatg
     7861 cgaagattgc cggcatgaag gggggatgtt tcaatggcga aaagggagaa ctacaacgag
     7921 ctgtacctgt ttatgcaggt ggtgcaagaa ggcagtttta ccgcagcggc taaacggctg
     7981 ggtcttgcgc agtcaggagt cagccgttct gttcgcgagc ttgaagaaag gctgggcgtc
     8041 cagctgctgg tgcgcaccac gcgtaaactg tctctgacgc aagcaggcga gcagctctac
     8101 cagaagacgg catctggatt tgaaatgtta gatcttgggc ttgccacact ggcgcactat
     8161 cgagagacgc cctccggcac ggtgcgtatc aatgccagcc agcacgcgat tgataaatgc
     8221 ctgctgccaa agctggcggt atttaaacag cgatatcctg atatccggct tgaactgata
     8281 aatgagagcc ggttcgtcga tatcatcgag gaaagattcg atgcaggtgt ccgcctgggg
     8341 cctgaagtcg gccagggcat ggtcgcggta cgcatcacgc ccgatatgga gatggcaatt
     8401 gtggggactc cggaacattt tcgtcgctat ggttttccgc aaaccccggc ggatttaaag
     8461 gcgcatccct gtatcgccta tcagtttgcc gacggcagcg tataccagtg ggaactcaat
     8521 caggatgata aaaaaatcac tcatcgccct gaagggcaat gggccctatc cgacagctat
     8581 atggaagcag aagccgcccg gctgggcctc ggattggcct atgttcctgt tgaactggtc
     8641 gcggatgatt tagaacacgg taagcttatc agggtgttgc agcgttacag cctgcgaatg
     8701 gagggattgt ttctctatta ccctcatcgc aacgtctccc ccgctctgcg gatggtcatt
     8761 gatacattga aaatctgaac gttaacaacg accgactctt cactgtgcaa tattgtgtca
     8821 ggtagtcact ctgcgttcaa ttccaggggc cgctttgagc gagaaagagc ggacttatct
     8881 gtacaagggg actatccagc aatggagatc atccaaaaga aaaaaccacc tggctcccct
     8941 cgacaacctt aatccgggca tttagcctca atcaacattt tcatatctgt caggttactt
     9001 cattatgaaa accgatcaat atcgttgttt ttgttgcaac agtagaatac gcgtatttat
     9061 ccctccattt ctattaataa cggcaataaa ctgttcattt cactgatatt taaaatatgc
     9121 atccttttca agctgttcag cggcataaga aagcgtatta aatatgaatt cgacgattta
     9181 accatttctg tctgattggt aatcatctgt gattaatgct ggtctattga ttacgccagc
     9241 attaaccgct tagggatgta ttcttcctga tcatatacag ccctcgaaaa agagagaaaa
     9301 taaaactgtg tccaatatta ctctaccact ccagaccgaa gcatcggtag aattagcaga
     9361 acgactggta cagaaggtgg gtatagcgtc atttgtcggg cattccattg ccatcataat
     9421 gataattttg aatcaataga cgtgcttctt gatcatgtct taaaaattat accatgagcc
     9481 ttatgaagca tcatttttac tgactggtgt taactttgct atcatataaa atctgttctt
     9541 aaaaaacgcg cccagaattt tatatgattt cccttgtttt ctgcctgtaa agaaattttt
     9601 ttcagtgctg ttaatggttg aaccgacggc agtaatcgtt ttggcgaggc tactgaatga
     9661 taataaagac agtgcaacca caacaattaa agaacagata gtattcatgt taattctcct
     9721 gacaattgag aggatgagtt ttatatacat aggtattaaa catgagtcat atttacccca
     9781 agagaaaaca ctccttctgt aattaaaccc tcatctcctt cggggtgagc aatacataat
     9841 gcatcttgct atgcaaaaca ggttgatata aattttgctt ctgacatatt taataaaacg
     9901 tgaatgtaaa aattattctt cagtttgcag atgcagtggg tctgatggta atttcggttg
     9961 tatcaacgct ttcgggagat tcaataatat gccttaccgc acgggcaata tccgcaggtt
    10021 gaagtgcaac agaacggtag gcgtccattg ccttcatggt ttccacatgg gtaatggttg
    10081 aagccagttc actttcgaca acgccaggat ttacgcaggt cacacggatt ttcgagcttt
    10141 cctgccgtaa gccatcagaa atagcccgta ctgcaaattt tgatgcacaa tagactgcgc
    10201 ctgtgggcac aacagaaagg gcaccaatag aaccaagatt gattatctga ccggaaccct
    10261 gtgcttccat caccggaagt acagcgccaa ttccccacag tacacctttg atattcacgt
    10321 caatagtgag ctcccattca ttctgttttc ctgctgcaag cggtgaaagc ggcataacgc
    10381 ccgcattatt gataagaaca tcaactcgcc cccagctatc cagcgctact tgcacgaaat
    10441 ctgccatgga ctgtcggtct gtgacatcca actcccgagc tttagcaatt cctcctgcgc
    10501 ggcaaatttc ggttgcaatg gcttcgatac gctcaactct gcgtgctccc agtaaagtct
    10561 ttgcacctgt cgttcccagc tctctggcaa taccttcccc aataccactt gatgcgccgg
    10621 tgattaatat gaccttatcc attacgttct ccttcaaatg tgttcggaga gagaatagaa
    10681 ctttatgatt ggttccagta tcctgctttc aatgtactca gtggttagcg gcattaaaca
    10741 atgaaaatcg atcttaatct tttacccatt tttatcgcgg tagcagaaga gagtaatttc
    10801 agtaaagccg ccgcacgact gggcgttacc cgttcagcgg tcagtcaggg aataagacgt
    10861 cttgaagatg cattcgggac aatgcttgta atgcggacca cgcgctcagt taatttgacg
    10921 gaagcgggag aacggctgca taaatcgttg tcattacctt tatccggtat tgaggcagcg
    10981 ttcgaagaga tggtttctga ccatgtgcca cgcggacttc tcagaatcgc ggtgacgtcg
    11041 atagcagaag aatttctttc aggtccgcta atcgcttcgt ttgcagctgc caatccagcc
    11101 gttacgcttg atattgttgt cacggatgag gaattcgata tcgtggccgc aggttacgat
    11161 gctggcgtaa ggctcggaga ggtcattgaa aaagacatga ttgctgtgcc ccttaccggc
    11221 aggcagcgtg aaatggtcgt tgcctctccc tcctatctcg cagccaacag cacacctgtt
    11281 cacccgcgtg aacttgtgaa tcataagtgt atcggctggc gtcagtcccc ggaggttgcg
    11341 ccttatcgct ggccatttga agaaaacggg agagctttct accttgcgat tgaaccgcag
    11401 attacgacga atgatctgcg tctgatgttg aggctggctc ttgccggcgg aggaataaca
    11461 attgccactc aggaaacgtt caggccatat attgaaagcg gtaagcttgt atcgctgctt
    11521 gatgactttc ttccacaatt tccgggcttc tatctgtatt tcccacagcg tcgcaatatt
    11581 gcaccaaagc tccgcgccct gattgaccac gtcaaggaat ggcggcagca attggcttaa
    11641 atgtctgcac ctgcactcta caagcagtgc tgagtatctg gatgtgccag aacggcttat
    11701 agaacttctg ttgaatcatg ttccgaaaga cagattaatc agaacttatc aggtcgggca
    11761 gcaggcggaa aaattacggg atcttttttt gaaatggggg gattttatta gcatgagatc
    11821 atcagatcta acaatgatgc tccagataat gttattcaga tcagctttgg aaagagatag
    11881 tttcgtattt ccacgagcag agatcgtcta atcactcagc gatctcttac tcgatggtgc
    11941 tatgtattga attaaatgaa tatgtttgag ttattgccac gaagttattc atataaccaa
    12001 agcatcatgg cccaattcag agtccctgaa cagcgctaag aatattactc cagcaccctc
    12061 tctagcttat tcaccgccgc cacatacttc gccggatttt tatctttaag caccgccaga
    12121 ttgcgttgct tccagcgaat agccggaaga tgctgaattt caggatagcg ataccgcgac
    12181 cagtcagggc tgttttgctt aaaactgagc aagaactcgc gatccagatc cgtaagatgt
    12241 gacttcaacg cctgcggcag caaagtcgtt actgacagca gcgattccag cgacgtttcc
    12301 tgctgagtca tccccgaaaa ttcctgagcg tataacgtgg caatccgatc tgtatcccag
    12361 tttggagcca gcaactcggc aatgggacgg ggatggcctg ccagataaca aataaaaccg
    12421 tcaaagatct cccgcatcag tcccggtttc tccagcatat tcagcacatc aaacagatcg
    12481 cgcgggtgtt gacgatcaag cgccgcgcag attttaccgc cataaagatc cgccagagaa
    12541 acgacactca tagtggtgaa gccatactcc atttccacac gttcgcacac cgacatttct
    12601 gccggaggca acaacagccc gcgccagact gggctaacct cgatcttaat ctttgcatca
    12661 gcggtattca caatgatgcg cttttcatcc gctttatttt cctgccgaat cgcggtaatc
    12721 cccggtttac tgttcagcga tgcagtaatt ctcttcaaag cgtcgttgat agccgccaga
    12781 tcggtatcgc gatccgcaaa gctcttatac gccagatcaa tatccaccga cagacgggga
    12841 aaatcctgca caaacaaatt gattgccgtg ccgcctttca acgcgaaaca ggactccttc
    12901 gccacatgcg gcagagcagc catcaacagc ttaacctgcc gggcataact atcacgcaga
    12961 tccatcttcg actccttccg gtttaaatgc gcgtggaaca gtaatttgat aatccggctc
    13021 cagccagcca ccgggtatga tttgccgctt tcccgtacct gtttctatac cgctttgatc
    13081 aagatactga aaataaggat gtccattacg ccgggcaagg aaaagcatca cccggttggt
    13141 tttaatcgaa tgactggcgc gaagcagtgc ctgcaatttg cgtgggctga gaagattcaa
    13201 cccctgaaac agagcatcgg catgttcgaa cgagattaac gaaggaacgc cgcaggcgat
    13261 ctcatacgca gccagttcca gggcactgcc tgctaactgc cggtcgccaa ttttcagttc
    13321 cgtagtaaat gacgtgacat caacggtaag gcccgatgta tacagcgtga taaatgttgc
    13381 aaattgttcg aaggcagcaa accatcccgg cagcttaacg taagccggaa gcgcgagcca
    13441 gacctgcggt ctgcccggtt cggtatagtg cgcatggccc tgtagcgcaa ggctggttag
    13501 ccccgctacc cgaacaggct tttcccactg tgtttgcagg cagtaaaccg cgtctaccca
    13561 atccggctca cggcccgtcc ggcagtacac gccgtgggcg atccgcctga gccagccatt
    13621 ctggacatag agataagaga gcttacgatc tatgccgttg cgggtaagcc atgactggag
    13681 cagcacctga ccgggtagcg tatgctgcat cagccagttt agatagtttg gcaaatgtcg
    13741 cctctgaata gacattaac
//
LOCUS       DAZUCE010000128        13696 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZUCE010000128 DAZUCE010000000
VERSION     DAZUCE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.128"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..288
                     /locus_tag="ACHY03_003218"
     CDS             <1..288
                     /locus_tag="ACHY03_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906412.1"
                     /translation="VTWDSDNIFTPNIFEHNGRCFMKKNETWMLLNGDATPNIRTMSN
                     KKIECLEKSEVEWASRVCDSITDKERENLKALICSKPAKSVNTGKRPALGY"
     gene            883..1110
                     /gene="pptA"
                     /locus_tag="ACHY03_003219"
     CDS             883..1110
                     /gene="pptA"
                     /locus_tag="ACHY03_003219"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFS3906413.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(1114..1683)
                     /gene="yddH"
                     /locus_tag="ACHY03_003220"
     CDS             complement(1114..1683)
                     /gene="yddH"
                     /locus_tag="ACHY03_003220"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFS3906414.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIEHNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQEQYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            1856..2701
                     /gene="nhoA"
                     /locus_tag="ACHY03_003221"
     CDS             1856..2701
                     /gene="nhoA"
                     /locus_tag="ACHY03_003221"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFS3906415.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVPDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            complement(2797..3690)
                     /gene="yddE"
                     /locus_tag="ACHY03_003222"
     CDS             complement(2797..3690)
                     /gene="yddE"
                     /locus_tag="ACHY03_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFS3906416.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHHDDYRISLEQGTPGFEPPLEDETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            3827..5017
                     /locus_tag="ACHY03_003223"
     CDS             3827..5017
                     /locus_tag="ACHY03_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4303 domain-containing protein"
                     /protein_id="HFS3906417.1"
                     /translation="MKQVQFDWKGMETAAINYMVASVKHIHQEHPCQHIYGAMFHCFY
                     GDGDIIDWPCLAVGTEESLASIVTAYQKNGFTQNVEKLTQDLRWSPADHKYNDEPNEE
                     CEQWANECHEFAAINGTFDAWYEVYAQFLRVFPKAAKKARLQLLREGVVEKNFIAIAS
                     DEVGELIPLSLTKKQILQYFPQYSQAEQERQYLKSLPSQARIAELIAQIFGRKTPGLL
                     LDDYEVLLKQLGQLALPALLQEVKNADKAWNACKLIAEINESNEDVIAVLTSLLDDQT
                     ADGSNRCWAAAAIARLGHMNLLVERISQLPVEILLRGVTAPYLAFRDHGRHLPLDYTP
                     LEFVLSHHPELVEAIAKEMAPGRGFCTITANEEPTARAGLVSSWPCIQFHAQYVLEEF
                     EDNH"
     gene            complement(5082..5762)
                     /gene="narI"
                     /locus_tag="ACHY03_003224"
     CDS             complement(5082..5762)
                     /gene="narI"
                     /locus_tag="ACHY03_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFS3906418.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMLKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            complement(5759..6454)
                     /gene="narW"
                     /locus_tag="ACHY03_003225"
     CDS             complement(5759..6454)
                     /gene="narW"
                     /locus_tag="ACHY03_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFS3906419.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            complement(6454..7998)
                     /gene="narH"
                     /locus_tag="ACHY03_003226"
     CDS             complement(6454..7998)
                     /gene="narH"
                     /locus_tag="ACHY03_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFS3906420.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            complement(7995..11735)
                     /gene="narZ"
                     /locus_tag="ACHY03_003227"
     CDS             complement(7995..11735)
                     /gene="narZ"
                     /locus_tag="ACHY03_003227"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFS3906421.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGDIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGELRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     KELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKSINWLDDEGRDQVQEAKK"
     gene            complement(11830..13218)
                     /gene="narU"
                     /locus_tag="ACHY03_003228"
     CDS             complement(11830..13218)
                     /gene="narU"
                     /locus_tag="ACHY03_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFS3906422.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            complement(13539..>13696)
                     /locus_tag="ACHY03_003229"
     CDS             complement(13539..>13696)
                     /locus_tag="ACHY03_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFS3906423.1"
                     /translation="VVNQRNIKIKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFS
                     SSSWPFE"
BASE COUNT         3462 a         3446 c         3478 g         3310 t
ORIGIN      
        1 gtaacatggg attctgataa tatattcaca cctaatatct ttgaacataa tggaagatgt
       61 tttatgaaga aaaatgaaac atggatgtta ttaaatggag atgcaacacc aaacattaga
      121 acaatgagca ataagaaaat agaatgcctg gagaagagtg aagtagaatg ggctagtagg
      181 gtttgcgaca gtatcacaga caaggaaagg gaaaatctga aggcattaat atgtagcaaa
      241 ccagctaaat cagttaatac aggcaaaaga ccagcgttag gatattgaaa ccggcactgc
      301 actctatcac tattacaata ttctgagtga ctagagcaac tgctccgcca tccgggctag
      361 cagtatgacg acgaatccga actgcactac aactggcacc ggtactacaa tccgggacag
      421 ggaccaggga aagatcactg tgtgccgtag gcacaaagat gcaaaacggg acgttttgca
      481 tgtaataagc ataagcttgt gaagagatgg tggcaggccc accgggatct gctgtcggaa
      541 gtcggtccca aacaaccccg gctgctgtgg taaagagcca tggttcgttg agtttttctg
      601 cattgccggt aattttggca tctcctttgt ccgtagcttg tttcagcgtc tataatgggc
      661 gttaataaat tggcaagtcg ggaacttgat gttttcttaa tggtatgtag gcagaagaat
      721 atgttctgca agttgacgat ttgttggtgc tttatttaaa agggtgtagt gcaaatatct
      781 acaatttcaa gtaggcatta ttgtcgtaaa aacgcatcac cggacatacg acattcacaa
      841 tgtgtgttaa ttttactgac atctttcaaa aggagcacaa tcatgccgca catcgacatt
      901 aaatgttttc cgcgtgaact ggacgaacaa caaaaagcag cacttgctgc agatattacc
      961 gacgttatta ttcgccatct gaacagtaaa gatagctcga taagcatttc tctacagcag
     1021 attcaaccag aatcatggca agctatctgg gatgccgaaa tcgcgcccca aatggaggct
     1081 ttgataaaga aacctggtta cagcatgtaa tgcttaaccc gccgtaacac gcttgccgct
     1141 agtaacaaac gtgccagcac ctaaatgatg caacgtattg agcttatcat catcaaactg
     1201 ccagcggcct tcgacaaata cccgtgcgtc tgccgctgct gatactacct cgccaaacag
     1261 tgtgtcgtat tgttcttgcg cagaagtcgc aggtagcagg cgacactcca tccacgccag
     1321 acatttttcc tcgaccagag gcaaaccaaa taccgggcct ctcacaaccg gaatgccata
     1381 gcaattaaat ttatcttctt cacgccccga cacacttccc actgcccacg tccagttagt
     1441 tgctgcaacg cccgggataa cgatgccaaa tttaccgtta tgttcaatca actccctggt
     1501 ccatgtcgat ttatctacca caatcgccac gcgtggaggt tcaaactcca ccggcattga
     1561 ccaggctgca gccataatgt tacgccgctg ggattgctca tcgaaactgg tgatcaagac
     1621 agttgggcca tgatttaaca gacggctggc atggtgtaat tctatgggga tgaatcggct
     1681 catggtatcg cctcgcgaag aaggtttttt taagcgtagt ccgtaacgca ataagtaacg
     1741 aaattaacgg gattggcgat ttgcgaacgt gatgcatgtc cgcgatcgca caaaatagcc
     1801 ggtgcggcgt ctattccagg ttataagttg agaaaaccac taagggaaac gcctgatgac
     1861 gcccattctg aatcactatt ttgcccgtat taactggtcg ggagctgctg cggtcaatat
     1921 tgatacgctt cgtgcattgc acctgaaaca caattgcacc attccgtttg aaaacctcga
     1981 cgttttgctg ccgagggaaa tacagcttga tgatcaatcg ctggaagaga aactggtgat
     2041 agcccgtcgt ggcggttact gttttgagca gaatggcgtg tttgagcggg tgttacgcga
     2101 gctggggttt aacgttcgca gcttgttagg gcgggtagtg ttatcaaatc cgccagcatt
     2161 accgccgcgc acccatcgtt tgctgttggt tgaactggaa ggggaaaaat ggattgctga
     2221 tgtcggtttc ggtgggcaga cgctaaccgc gccgattcgt ttagttcccg atctcgtgca
     2281 gaccacgcca cacggagagt atcggttgtt gcaggagggt gatgattggg tgttgcagtt
     2341 taatcatcat cagcattggc agtcgatgta ccgttttgat ctctgcgagc agcaacaaag
     2401 cgattatgtg atgggcaatt tctggtcggc gcactggccg cagtcgcatt ttcgccatca
     2461 tttgctgatg tgccgccatt tgccggacgg cggcaagctg acactgacca attttcattt
     2521 tacccattat gaaaatgggc acgcggtgga gcagcgaaat ctaccggatg tggcgtcatt
     2581 atatgctgtg atgcaagaac agtttggtct gggcgtggat gatgcgaaac atggctttac
     2641 cgtggatgag ttagcgctgg tgatggcggc gtttgatacg cacccggagg cgggaaaata
     2701 atttatgtca ggttgccgga tgcggcgtaa acgccttatc cggcatacat tagctcggtt
     2761 gtagacctga taagcgaagc gcatcaggca acggtttcag agtttaattg cccattcggc
     2821 atgaaacaaa atcaccgccg cgccagaaat ggtgactttt tccggttggt tatcacgaat
     2881 tgtcaccgtc acttcaatca cgccgtcacg ccccaatgcg cgcccctgat ggcctttaac
     2941 acgcagcacc ttgccatcgt ggggcaatac gttgtgatgt accaaccatg cacccatcgg
     3001 gccattggca ttgccagtca ccggatcttc cacaatacca atcgcaggcg agaacatgcg
     3061 accatcggtt tcgtttttgc ctgggcggat ctggaacggg aagaaaccat tgcagccaat
     3121 ctgtttgctg atagcgctca gcgcagcaag atcaggcgaa agggcgtcga tatccacttc
     3181 tggtttcaac gggatcatca cttttgagtg tcctgttgtt gccacctgaa ttggcaagcc
     3241 tggcagaata tcgtcctcag tgagatgaag cgcgttgata atcgccgcac gtgtttcatc
     3301 ttccagcggt ggctcaaagc ccggcgtacc ttgttccagc gaaatacgat aatcatcgtg
     3361 gtgcttttcg atagtcacgc gatgttttcc tgccagcgat gtttgccaga ccgtgcaatt
     3421 tcctaaacct aacaccttcg cacgtacata gtgcgcagct actgtcgcgt gaccacaaat
     3481 cggaacttca accgttggcg taaagtagcg gatgcgcaca tcgctgtcgt cgctgtgcag
     3541 cagaaaagca gtttccgaat gacctaattc gcgggcgata agctgcattt gcgcttcgct
     3601 aagattatcg gcggggaaaa caacaccggc agaattgccg cgaaatggtt gagaggtaaa
     3661 agcatcgacg tggtaaacct gcggtttcat taacgttctc ctgtgactgg agaactatca
     3721 tagcctgttt agggcacttt tctgaattaa tccttggcaa gtcatgttga aataactatc
     3781 aggataaaat ggttaactct ctatattgcc ttacacggac cgctttatga agcaagtaca
     3841 atttgactgg aaagggatgg aaacggctgc cattaattac atggtagctt ccgttaaaca
     3901 tatacatcag gaacacccat gccaacatat ctatggtgcc atgtttcatt gtttttatgg
     3961 ggatggggac atcattgact ggccttgtct ggcagttggc actgaggaaa gcctggcctc
     4021 tattgttact gcttatcaga aaaatggatt tacacaaaac gtagaaaaac tcacacagga
     4081 cttacgctgg tcgcctgcgg accataagta caatgatgaa cctaacgaag aatgcgaaca
     4141 atgggctaat gaatgccatg agttcgctgc tattaatgga acatttgatg cctggtatga
     4201 ggtgtacgcg caattcctgc gtgtttttcc gaaagcagcc aaaaaagcac gactacaact
     4261 cttacgtgaa ggggtagtgg agaaaaactt tatcgccata gcctctgatg aggttggtga
     4321 gttgatcccg ttaagtctga caaaaaaaca aatattgcaa tattttcctc aatattctca
     4381 ggctgaacag gagaggcaat atctgaaaag tttgccttca caagcacgga tagcagaatt
     4441 aatcgcgcaa atatttggca gaaaaacgcc gggtctactt ttagacgatt acgaagtatt
     4501 actcaaacaa ctaggtcaac tggcgttacc tgctttgctg caagaagtta aaaatgcaga
     4561 caaagcatgg aatgcctgta aattgattgc agaaattaat gaatctaatg aggatgtgat
     4621 tgctgttttg acaagcctgc tggacgatca aacagccgat gggagtaacc gctgttgggc
     4681 tgcagcggct atagcgcggc ttgggcatat gaatttattg gtagaacgca tttcccagct
     4741 ccctgtagaa attttgcttc gtggagtaac agctccatat ctggcatttc gggaccacgg
     4801 acgtcattta cccttagatt acaccccgtt agagtttgtc cttagtcatc atccagagct
     4861 ggttgaagcc atagcaaaag aaatggcacc aggaagaggg ttttgcacca tcaccgccaa
     4921 tgaagaaccc actgctcgcg ctggtttagt ttcatcctgg ccctgtattc aatttcatgc
     4981 tcaatatgtt ttggaagagt ttgaagataa tcattaaggt tgaaaagcct gtttttctga
     5041 caacacatga caagaaaaac aggcggtttt gaaaagcaga attatcgccg cgagcgcaca
     5101 atctgatacc gacgagtaaa gtactcaaac ggggcgctcc acacatgcac cagtctagtg
     5161 aacgggaaga gcaggaagat ggtcattccc aacaccagat gcaggcggaa gacgaacgct
     5221 acaccgttaa gcatctctga cgatccacca cggaaagtga caatgctttg cgcccaaccc
     5281 accagcttca acatttcgct accatcagga tactgtgctg aaaacggtat ggtgcttagc
     5341 cccaacagac actggaccag caaaatgctc atgatgatga tatccggcgt agtggaagtg
     5401 gcacgcacgc gctgatttgt cagcctgcgc cacagcagtc ctgcgccgcc aatcaacgtc
     5461 aacacaccgc aaataccgcc gagcaccatc gccattagtt gtttcgctgc cactggcaaa
     5521 aaccacgcgt acatccagtg cggcgttaac atgccgaaca ggtgcccgaa gaagatcccc
     5581 aaaatgccga tatggaacag attcgaccat atcaccatcc cgcgtttatc gagcatttga
     5641 cttgaggagg cgcgccaggt gtactggcca tagtcgtagc gcagccagct gccgaggaaa
     5701 aacaccgtcg cacaaatgta cgggtagata tcgtaaaaaa agacgttcag atactgaatc
     5761 atttcccacc tcccgcactg atatcgacat attgcggcgc gacatcctgg ctaaagcgtc
     5821 gctgatattg attaagcggc gagctgtcac acgccgtggc gttatcttca ataaacttca
     5881 cctgttcctc ttcccacact gcatccagcg cctggcgggt gtcatcgcgc tcttcgctgt
     5941 ttacttgttt tgtgacactg tcacttgaaa gtgtacttcc cgccagttgc agcagagcat
     6001 caaacaacgc gtaccacggt gcctcgcgtt gttttaagcg accgccaagc agggcgagga
     6061 tcggcgcaac attgagcaat ccttctttcg cctgatcgtc aggcagtacg cttaaatact
     6121 ccagatacag cggtagataa tcgggcagtt cccgacaatc cagctgcagg ccgacctttt
     6181 catactccgc cagcaggtcc accattgcct ggccgcgatc gcgggactcg gcatgaacat
     6241 gttcgaacag cagcagcgac gtagtgcgcc cgcggtcaaa cacttcgcac cattcggcct
     6301 gtttatccag cagcggcgcg ttaagcaggt tgtgggtgaa atccgtaagc atcggcgcgt
     6361 cgcggcggat caacgccagc gcgtcctcct tgcattccca caacagctcg tccggatact
     6421 ccatcaacag gccgatcact ttgaggatct gcattattcg ccctccgctt tgtcgcgcac
     6481 ttcggtgatg ttgatggcat cgatacggct actgttgaac aggttgaatt tactgtccga
     6541 accgtggcaa ccgtcgccaa aggtaaaacc gcagccgttg cgttctgcga aggcatcgcc
     6601 cgccatttcc cgatggctgg tcgggataac aaaacgatct tcatagttgg caatggcgag
     6661 atagcgatac atttcttcga cctgggcgac gctcaggcct acttcgtcga tggcacgagt
     6721 atcagtaacg ccttccacgg tttgtgaacg catatagtgg cgcatcgcca tcatccgttt
     6781 cagcgcccgc agtaccggac cagtatcacc ggcactcaac atattggcga gatattgcac
     6841 cggaatacgc aggctttcga tggcggggag cacgccttcg cttttcggca aaccgcccgc
     6901 atcagcgtaa gactgaatcg gtgacagcgg aggaacgtac cagaccatcg gcagggtgcg
     6961 gtattcaggg tgcaacggta gtgccagttt ccagtccatc gccattttgt agactggcga
     7021 acgctgggca gcgtcaatca cattttgtgg aataccttgt ttcagggctt cctcgatcac
     7081 tgagggatcg tgtggatcga ggaacacttc gcactggcgc tcatagaggt caacttcgcg
     7141 ctcggtgctt gccgcttcct caatgcggtc ggcgtcgtaa agcagcacgc ccagataccg
     7201 gatgcgaccc acgcaggttt ctgagcacac ggtcggttga ccggactcaa ttcgtgggta
     7261 acagaagata catttttctg acttgccgct tttccagttg aagtagattt ttttgtacgg
     7321 acaaccgcta atgcacaaac gccagccacg gcatttatcc tgatcaatca gcacaatgcc
     7381 gtcttcttcg cgtttgtaga tagcgccgct tgggcaggtc gccacgcagc tgggattgag
     7441 gcagtgttcg cacaggcgcg gcaggtacat catgaaggtg ttttcaaact gcccgtacat
     7501 ctccttttgc atggcctcga agttgcggtc gcgggcacgt ttttcaaact cgccgcccag
     7561 cagttcttcc cagtttggcc cccagatcac tttgtccatc cgcttaccgt caatcagcga
     7621 acgcgggcga gcagtaggga tatgtttgcc ttccggtgcg ctatgcaaat gttcgtagtc
     7681 gaaggtgaaa ggttcgtagt aatcatcaat ctgcggtacc accgggttgg cgaagatttt
     7741 ggttattacg cccatcttgc tgcccagacg cgggcgtatc ttgccattca catcgcggac
     7801 ccagccgcct tgccactctt cctgatcttc ccagtttttc ggataaccaa tgcccggttt
     7861 ggtttcgacg ttgttaaacc atgcgtactc catgccttcg cgtccggtcc agacgttttt
     7921 acaggtcacc gaacaggtat ggcagccgat acatttatcg agattaagca ccatgccgac
     7981 ttgcgaacgg attttcattt tttcgcctcc tgtacctgat cgcgaccttc atcatccagc
     8041 cagttaatgc tcttcatctt gcggatcatg atgaattcgt cgcggttcga accgacggtg
     8101 ccgtagtagt taaagcccca ggccagctgt gcgtaaccgc caatcatatg cgttggtttc
     8161 ggacaaacgc gggtgaccga gttatgaata ccgccgcgca tgccagttac ttccgaacca
     8221 ggaatattca taatgcgttc ctgagcgtga tacatcatgg tcatacccgg cggtacacgt
     8281 tggctgacca ccgcgcgggc cgtcagcgcg ccattggcgt taaacacttc cacccagtcg
     8341 ttatcgacaa tggttagttc acgggcatcg gtttcgctga tccagacaat cggtccaccg
     8401 cgagagagcg tcagcattag caggttttca ctgtaggttg agtgaatgcc ccatttctgg
     8461 tgcggcgtca ggaagttaag tgctttttcc gggaagccgt ttggcgggat ctggcgcatc
     8521 tcactgacgc tacgggtgtc gataggcggg cgataagcca ccaggctttc accaaaagca
     8581 cgcatccacg gatgatcctg atagagctgc tggcgtcccg acagcgtgcg ccacggaatt
     8641 aactcatgaa cgttggtgta tcccgcatta taggagacgt gatcgctttc cagaccagac
     8701 catgtggggc tggagataat tttacgcggc tgcgcctgaa tatcgcgaaa gcgaatcttc
     8761 tcgtcctctt tgtgcagcgc cagatgggta tgttcgcgtc cggtgatctc gcccagcgcc
     8821 tgccacgctt taactgcaac atgaccgttg gtttctggtg ccagtgccag aatcacttct
     8881 gaagcgtcaa tggcggtgtc aatcagcgga cgtccctgcg ctgggccatc acgcttggtg
     8941 taattgagtt taccgaggaa atcgatttca tcctgcgtat tccacgaaat ccctttaccg
     9001 ccgttgccaa gtttgtccat caatggcccg agcgaggtaa agcgttcata cgtagcaggg
     9061 tagtcgcgct ccaccgccac aatattcggc gcggttttac ctggaatcag atcgcattcg
     9121 cctttgcgcc agtcgagcac ttcacacggc tgtgagagct ctgccggaga gtcgtgcagc
     9181 agtggttgca gtaccacgtc ggtttctttg ccgagatgac ccacgcacac ttgtgaaaat
     9241 gctttggcaa tacctttgta gatttcccag tcgctgcgtg actcccaggc cggatcgacc
     9301 gccgcagaaa gcggatgaat aaacggatgc atatccgagg tattcatatc gtctttttcg
     9361 taccaggtgg cggtaggcag aacaatatcg gagaacaggc aggtgctgga catgcgaaag
     9421 tcgagcgtca ccagcaggtc gagcttgcct tcaatcgccg cagtttgcca ctcgacttct
     9481 tccggtttga tcccgtcgct ggcaccgagt tccttgccct gaatcccgct ttcggtcccc
     9541 agcagatact tctgcatata ctcgtggcct ttgccggagg agccaagcag gttagaacgc
     9601 cagacaaaca aattacgcgg atgattgctg ccgctatctg gttgttcgca ggccatacgc
     9661 aactcgcccg atttcagcgc ctgggcggta aattctgtgg gggataatcc ggccttgtcg
     9721 gcttcagctt taatcccgag cgggttacgc cccagctgcg gcgcagaagg tagccagccc
     9781 atgcgttcgg cgcgaacgtt gaaatcaatc agatgaccgc tgtacttact ggcatcggca
     9841 agcggtgaaa gtaactcctg cgcagagact ttttcatagc gccattggct ggaatgattg
     9901 tagaaaaacg aggtgctgtt catctggcgt ggtggtcggt tccagtcgag cgcaaaggcc
     9961 agcggcaacc agccggtttg tgggcgcagt ttttcctgac cgacatagtg cgcccagccg
    10021 ccgccgcttt gcccgacaca accgcagaag atcagcatat tgatcatccc acggtagttc
    10081 atatccatgt gataccagtg gttgacgcca gcaccgagga taatcattga gcgcccatgc
    10141 gttttatggg cggtatcggc aaattcgcgg gcgatagttt caatatactg gcgcggcacg
    10201 ccggtaattt gctcacccca ggctggggtg tacggtttga tttcagcgta atctttcgcg
    10261 ctgttttcat cttccagccc gcgatcgagg ccgtaattcg ccagtaccaa atcataaacg
    10321 ctgaccactg gacaggtgct gccatcggct aatgtcaggt ttttaacggg taattggcgc
    10381 accagcaccg gattgtgttt tacgctgcga aaatgcggat tttcgatgtc gccaaagtag
    10441 gggaaggcca cgcctgcaac agcgtcatgt tgaccgagca gggttaacga caattcggtt
    10501 tccttaccgg cggcaatgga ttccagattc catttgcctt tttctcccca gcggaaacca
    10561 atcgaaccgt tcggcactac caattcacct gcggtattaa ccgccacggt tttccactgc
    10621 ggattattgc tttcgcccag tccatctacc aggtcagacg cgcggatcat gcgcccgggt
    10681 acgtagctac catcgtcgcg aggttccagc attaccagca tcggcatgtc gctatagcgg
    10741 cggcagtagt tgataaagta gtcgctggga ttatcgagat gaaactcttt taaaatcaca
    10801 tggcccattg ccatcgccag ggcgctatcc gtgccttgtt ttggtgccag ccactggtcg
    10861 cacaatttgg ccacttcaga gtagtcaggg gtaatggcga tggttttagt gcctttgtag
    10921 cgtacttcgg taaagaaatg ggcgtccggc gtacgtgtct gcggtacgtt agacccccag
    10981 gcgatgatgt aactggagtt ataccagtcg gcagattccg gtacgtcggt ttgctcgccc
    11041 caggtcatcg gcgaggcggg cggcaggtcg caataccagt cgtagaagct taaacaggtg
    11101 ccacctaata gtgacagata acgtgttccg gcggcgtaag aaaccatcga catcgccggg
    11161 atcggcgaga aaccggcaac gcgatccggg ccgtaggttt tgatggtcca gacgttagcg
    11221 gcggcaatca gctggtttag ttcctgccag ttggagcgga taaacccgcc gcgcccacgc
    11281 acttgtttgt agctcaggca cttttgcgga tcgttcataa tcgatgccca cgccagtacc
    11341 ggatcgctgt gttgtttgag ggcttcgcgc cacagttcaa tcagtctttt acgaatgagc
    11401 gggtatttca ggcggttagc gctgtaaaga taccaggagt aacttgcgcc acgcgggcag
    11461 ccgcgaggtt catgattggg caggtcaggg cgagtgcgcg ggtagtcggt ctgttggatt
    11521 tcccaggtca ccagaccatt tttaacgtag attttccagc tacaggagcc tgtacagtta
    11581 acaccgtggg tggaacgcac gattttgtcg aactgccaac gctggcgata gctgtcctcc
    11641 cagtcgcggt tgctatgcat cacctgtccg tgaccatcgg caaaggtttc gcccttttgt
    11701 ttgaagtagc gaaagcgatc caaaagttta ctcatgacat gactcctgct ccaggaatga
    11761 attgagatct gatgccttgt cggatgcgac acggttgtag cctgataaga cgtgtcaggc
    11821 atcgatctct tatttttggc tgaacttccg ccgaccataa accagccagg tcagtagcac
    11881 acaaacgatg tagaagatta aaaacacttt catcgcgccc actggagagc cggtcatatt
    11941 gagcgacatg ccaaacgcct gcggaataaa aaagccgccc actgcgccaa tggctgagat
    12001 aaagcccaga gccgccgccg tttcggtgac ggcttctttt tgagcttgct catcactacc
    12061 gcctttcatc ttcacccgat aaatggttat ctggcgaaag atgacggcga tcatctggaa
    12121 agtagaacca cttcccagac ccgcggtcag aaacagcccc ataaatacgg cgtaaaaggc
    12181 gatgaaatta ccggagcctg tgcccggtaa ggtaaggaac agcagggcgc tgaaaatcgc
    12241 cataaaaata aagttgatca acgtcacccg cacgccgccg aatttatcgg aaatagcacc
    12301 accaaccgac cgcgcgatgg caccgataaa tgggccaaag aacgccaggc gcagaatatt
    12361 cacatccggg aactgggttt ttgccagcat ggcaaaaccc gcagaaaaac cgataaacga
    12421 accgaaggtg gcaaggtaaa gcaggctcag cagccagaga tgcaggcgtt gtaagacagg
    12481 gagctggtcg gcaattgacg cgcgtgaact ggcgatatca ttcatccctg accaggcggc
    12541 gatcgtggca atcgccagta gcggtaccca aatccatgcg gcattcgcca gcgacatcac
    12601 cgaaccgtcg gcctgcggga cgccattgac gccgagaaag gcaaatacgg gtacaaaaat
    12661 gaccagcggt gcaaccagct gcatcacact tacacctaag tttcctaatc cgccattaat
    12721 cccaagagcg ctcccttgcc tggcttttgg aaagaagaaa ctgatattgc ccatgctcga
    12781 agcaaagttc gcacctgcaa aaccgcatag caaagcgata acgataaaga tcccaaaagg
    12841 agtattcgga ttctgcacgg caattccgag ccagacgcaa ggaataatca ggattgcagt
    12901 actaaaaacc gtccatcggc gtccaccgaa tataggtacc ataaaggagt agggaacacg
    12961 caataatgcg ccagaaacgg agggtaatgc ggttaataaa aagagttgat cggtagtaaa
    13021 attaaaaccg attttattga gattaacggt aactgcgcta aatagcatcc agacacagaa
    13081 ggcaagaagt agacaactga ctgatatcca gaggtttctt cgagcaatat gttttccttt
    13141 attttcccag aaggccggat tttctggttt ccagtcgcgt aaaagataac gactattttt
    13201 ctcattttgc agtgccatat tgttcctcac atgcacacat tggtaatgaa aaaagacaaa
    13261 acaggaggta aggcgcaata gccagttatt agaattaagg atgaattggg tgaagtgctg
    13321 attgaaagaa tagataagaa agcgtaacag cgggggcaga atggggatta ggtagccaga
    13381 tatgtgttac taaatgtaac tgacaagcta accattaaac ggggggtaat ctttgaacgc
    13441 atgtcatgat acaacccttc agatgatgaa tccaaagggt tgtatattaa ctatattctt
    13501 aacaagacaa gacacttata gaattagcaa taatttattc actcaaaagg ccatgagctg
    13561 gaggaaaacg atctgtccag aaggtctatc ggttcctctc ttattgcctg attgtaataa
    13621 tcgttattgc agtaagtatt gtttatttca tctaaggtca tgtttttaat ttttatattc
    13681 ctctggttta caactt
//
LOCUS       DAZUCE010000129        13365 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZUCE010000129 DAZUCE010000000
VERSION     DAZUCE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.129"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(19..171)
                     /locus_tag="ACHY03_003230"
     CDS             complement(19..171)
                     /locus_tag="ACHY03_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3906424.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(435..1169)
                     /gene="cysH"
                     /locus_tag="ACHY03_003231"
     CDS             complement(435..1169)
                     /gene="cysH"
                     /locus_tag="ACHY03_003231"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFS3906425.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1243..2955)
                     /gene="cysI"
                     /locus_tag="ACHY03_003232"
     CDS             complement(1243..2955)
                     /gene="cysI"
                     /locus_tag="ACHY03_003232"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFS3906426.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESQKAKIEKIAQASGLMNVVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2955..4754)
                     /gene="cysJ"
                     /locus_tag="ACHY03_003233"
     CDS             complement(2955..4754)
                     /gene="cysJ"
                     /locus_tag="ACHY03_003233"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFS3906427.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQTAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYLPGDAL
                     GVWYQNDPALVKELVELLWLKGDETVTVDGKTLTLSEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDADALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAVDEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5073..5435
                     /gene="queD"
                     /locus_tag="ACHY03_003234"
     CDS             5073..5435
                     /gene="queD"
                     /locus_tag="ACHY03_003234"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFS3906428.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5513..6784
                     /locus_tag="ACHY03_003235"
     CDS             5513..6784
                     /locus_tag="ACHY03_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS3906429.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVVEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTYPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6775..7035
                     /locus_tag="ACHY03_003236"
     CDS             6775..7035
                     /locus_tag="ACHY03_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFS3906430.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTVQRLINACPAGLFSLTPG
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7052..7627
                     /locus_tag="ACHY03_003237"
     CDS             7052..7627
                     /locus_tag="ACHY03_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFS3906431.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7774..8634)
                     /locus_tag="ACHY03_003238"
     CDS             complement(7774..8634)
                     /locus_tag="ACHY03_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFS3906432.1"
                     /translation="MNIAIVTINQENAALAGWLAAQDFSGCTLAHWQIEPQPVVVEQV
                     LDALVEQWQRTPANVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGTDKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARWVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVCNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8631..9410)
                     /locus_tag="ACHY03_003239"
     CDS             complement(8631..9410)
                     /locus_tag="ACHY03_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFS3906433.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFASE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCWVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAELPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9388..10797)
                     /locus_tag="ACHY03_003240"
     CDS             complement(9388..10797)
                     /locus_tag="ACHY03_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3906434.1"
                     /translation="MMGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGVLLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10819..12273)
                     /locus_tag="ACHY03_003241"
     CDS             complement(10819..12273)
                     /locus_tag="ACHY03_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFS3906435.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12343..13128)
                     /locus_tag="ACHY03_003242"
     CDS             complement(12343..13128)
                     /locus_tag="ACHY03_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3906436.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKKMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
BASE COUNT         3106 a         3651 c         3362 g         3246 t
ORIGIN      
        1 tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct taaactccat
       61 accacgctct ctgatactca actcacataa cgagtcgcct accatgagcg tgaatcccag
      121 tactgtacaa cacagtacta tgattgccac aagggcatat tttgttagca tgtcttgcct
      181 ccctgcaaag aagagactaa catccgaatt gctacgttcg aacgcgagcc tcaggttgat
      241 agaaatatcg cctggggctt ttgtccatct ggaacctcgc gaatgcttaa cgccagacag
      301 cctcaagcac ccaacgccat tctatacctg ataattcttc ctgcgacgtt cacctttaaa
      361 tgccggaatg ctgctcgcaa aataaaaacg cccccatcat ttctgacaga ggcgtttaat
      421 ttatccggca atatttaccc ttcgtgtaac ccacattccc tttttaagcc aaagaaacgc
      481 gtttcttctt ccgccatgcc gggttcccat ttacgggttg tatgggtatc gcccaccgat
      541 aaatatcctt cgtcccataa tgggtgatat ttcaggccat gcttttgcag atactgatat
      601 atcgttcggt tatcccagtc gataatcggc agcactttaa atacgccacg ctgaattgcc
      661 agcaccggta aattggcgcg gctgccggat tgttcgcggc gcaggccagc aaaccacgtt
      721 tgcgcgttaa gttctttcag cgcccggttc atcggttcga ctttgttgat gtcattgtac
      781 ttttcaatgc cttcaacgcc ctgctcccac agttttccgt agcgtgcttc ctgccaggct
      841 gcgctttcgg tagcacggta cactttcagg ttgagcttga gtttgtccgt taactcgtca
      901 ataaagcggt aggtttccgg gaacaagtaa ccggtatcgg tgaggatcac tggaatatcc
      961 gggcgaatct gattcaccag atgcaggctc accgccgcct gaatgccgaa gctggaagaa
     1021 agcacatatt caccgggcag attatccagc gcccaggcta cgcggccttc agcgtccagt
     1081 ttttccagtt gggcgttagt ttccgccagc gccagaatgc gatctacctt cggcagttca
     1141 ttcagggcgt ttagatcgag tttggacata gattcctcac tgtttgcctt gcctgatgcg
     1201 acgctcacac gtcttatcag gcctacaaga ccgggctgat ggttaatccc acaaatcgcg
     1261 cgccggatcg agcaccgggc gaatgatgcc cgcacgcacc gtaaagtcgc cgaagccttc
     1321 acccgcttcg cgctctttcg cccagcgccc tatcagttca tcaagcgacg ccaggatttc
     1381 cggctcggtg atgttttctt tatacatccg tgggatacgt gtcccaatgc ggttgccgcc
     1441 aagatgcagg ttgtagcgac ccggcgcttt acccaccagg cccacttccg ccagcatcgc
     1501 gcgcccacag ccgttcgggc agcccgtcac acgcatcacg atatgctcat cactgacgcc
     1561 atgttttgcc attaaattat cgatattgtc gataaacgac ggcaggaatc gctcagcttc
     1621 ggccatcgcc aacggacacg ttgggaacga cacgcaagcc attgagtttt cacgctgcgg
     1681 agtgacgaca ttcatcaggc cgctcgcctg cgcaattttc tcgatcttcg ctttctgact
     1741 ttccggtacg ccggcgatga tcagattctg gttcgccgta atgcggaaat cacctttgtg
     1801 gatcttcgcg atctccagca ggccggtttt cagcggacgc cccggataat caaggatgcg
     1861 accattttcg ataaacagcg tcaggtgcca gttatcatca atgcccttaa cccagccaat
     1921 acgatctccg cgtccggtaa actcatacgg gcgaatcggc tcgaatttga tccccgcgcg
     1981 acgctccact tccgctttaa acgtctcaac ccccacgcgc tccagcgtgt atttggtttt
     2041 ggcattttta cgatcggttc ggttacccca gtcacgctga gttgtcacga cggcttccgc
     2101 caccgctagc gtatgctcta gcggcagata gccaaactca ctcgccgtgc gggcgtaggt
     2161 tttcttgttg ccgtgttcga tggaaagccc accgcccacc agcaggttaa agcccaccag
     2221 cttgccgttt tcggcgattg ccacgaagtt catgtcgttg gcgtgcagat cgatatcgtt
     2281 ctgtggcggg atcactaccg tggttttgaa tttacgcggc aggtaggtct ggccgaggat
     2341 cggttcttca tcagtggtgg cgactttttc ctgatcgagc cagatctccg catacgcgcg
     2401 ggtacgaggc agcagatgtt cagagatttt cttcgcccac tcgtacgctt ctgcgtgcag
     2461 ctgcgactcg taagggttcg aggtgcagag tacgttacgg ttcatgtcgt tagcggtcgc
     2521 cagcgcatca agaccgaccg agtgcagcat ctggtgcacc ggtttgacgt tctttttcag
     2581 aatgccgtgg aactggaatg tctggcggtt ggtcaggcga atgctgccat agatggtgtt
     2641 ttcaccggca aatttatcga tcgcctgcca ctgtttggtg gtgataactc cacccggcag
     2701 acggcagcgc aacagcatcg catggcgcgg ctccagcttc tgttcagcac gttcggcgcg
     2761 gatgtcgcgg tcatcctgct gatacatgcc gtggaagcgg atcagcagga agttatcgcc
     2821 cttaaagccg ccggtcaggc cgtcgtttaa atcttccgca atggtgccgc gcaggtagtt
     2881 gctttcaagc ttcatgcgct cggcgtctgt cagttttcct tcgaccacta aaggccctgg
     2941 atgtttttcg ctcattagta gacatctcgc tgataacggc gctctacgcg cagctcactt
     3001 aaaaattcat ccgccgcttc ggtgtccatg ccaccaaatt ctgcaatcac ttccagaagt
     3061 gcctgctcaa cgtctttcgc catgcgatta gcgtcgccgc agacataaat gtgggcaccg
     3121 tcattgatcc agcgccacag ctccgcgccc tgttcgcgta gtttgtcttg tacgtaaact
     3181 ttttcttttt gatcgcgcga ccaggcgaga tcgatacgtg tcagcacgcc ctctttgacg
     3241 tagcgctgcc actccacctg gtagaggaaa tcttccgtaa aatgcgggtt accaaagaac
     3301 agccagtttt tacccggtgc ttcatcgaca gcacgttgct gcataaaggc gcggaacggc
     3361 gcgataccgg tgcctgggcc aatcataatc accggggttt ctggattggc tggtaggcgg
     3421 aagttatcgt tatgttcgat aaatacgcgg acttcgccct cttcttccac gcggtcagcg
     3481 aggaagctgg aggcaccacc ggcacgggcg cggccttcca cgtcgtaacg caccacacca
     3541 acggtgacgt gtacttcgtt ctcgacttcc gcctgcgatg aggcgatgga atagaggcgc
     3601 ggcgtcagcg ggcgcagcag gttaatcagc gcgtcagcat caagctgtgc cggagagaaa
     3661 cgcaccatat cgacaatcgg cgtggtcgcg gcgtaatgct gcaacttcgc tttatcgccc
     3721 accagcggca gcagcgtttc actgcgggta agcgtggcat agttctcaac aatattggcg
     3781 gtgttaaccg tcagttcgaa gtgccactgt agcgcttcgc tcagggtcag cgttttgccg
     3841 tcaacggtga cagtttcatc acctttcagc cacagcagtt cgacaagttc tttcaccagt
     3901 gccggatcgt tctgatacca cacgcccagc gcgtcgcccg gcagatagcg caggccagag
     3961 tcacctaagt caatttcgat atggcgaacg tctttttcag agttacgccc ggtaattttc
     4021 tggttaacag agaggctagc caccagcggc gcgtctttgc tgtacgggct ggtgtggatt
     4081 tcatttaccg cgccagtagc gacggattgc gaaggtgccg cgacaggcgc acgcgattta
     4141 agcgcatcaa ccacgcgggc gcgccactcg ctggcagcgg tctggtattc aacgtcggca
     4201 tcgacacggt cgagcaggcg ttcaccgccc agttccgcca gcttgctgtc aaaatctttc
     4261 ccggactggc agaaaaattc ataagagcta tcgccgaggc taaacacggc aaatgcggtg
     4321 ttttccagct tcggcgcttt tttggagaac aggaacttat gcagcgcgac ggcttcttcc
     4381 ggcggttccc cttctccttg cgttgacgtc accacgataa gcagtttttc gctggcgatt
     4441 tgtttgaatt tatagtcgcc cgcgttcacc agcttaacgt tcagttttgc cgctaataaa
     4501 tcatcgcgta atgcttcagc aacccggcgc gcattgccgg tttgcgaggc ggagataata
     4561 gttatacccg gcatttctgc ggctggcgct ggcgtcgctg caagcgcagc aggctgctga
     4621 ttgagcacgc cccagaaata gccagaaacc caggcaagct gggtgggagt taaatcggtc
     4681 gtggccgcct gaaggcgtgc cagttgctcc gggttcaacg gaagcaacgc ggaaggtggg
     4741 acctgtgtcg tcatgcgtcg ttatgttcca gtaagcaaag ctgtttctgc gccctgtcag
     4801 cgcccataaa acagaagaga aggtaaggtt aacggggtga acggtgtgga ttaaagacgg
     4861 gatagcgata actaataacc aaatcgacta acctgtttta gcaatagtct ttaacaacaa
     4921 aatagattaa ccaacctaat gaaaaacaaa tgaatttagc caatcattaa gataaatcag
     4981 cgattttgcg caacaaagtc gttttagata atgcgaaaaa acagcctttc cggtactcta
     5041 cggcggtttt atcgtcctgt agagaaatta tgatgtccac cacgttattt aaagatttca
     5101 ccttcgaagc cgctcaccgc ttaccacacg tcccggaagg gcataaatgt ggtcgcctgc
     5161 acgggcattc ctttatggtg cgactggaaa ttaccgggga agtcgatccg catacgggct
     5221 ggattatcga tttcgctgaa ctgaaagcag cgtttaagcc aacttatgag cgcctcgatc
     5281 accattatct caatgatatt ccaggcctgg aaaacccgac cagcgaagtt ttagcaaaat
     5341 ggatttggga tcaggttaaa cccgttgtgc cgctgttaag tgcggtgatg gtaaaagaaa
     5401 cctgcaccgc tggttgtatc tatcgcggcg aatgataaga gtgtgtcggc ggtcaatttc
     5461 ccttaagtaa cgctatgtta gggtgttgtg ttctggatat ctggggcatg acatggaaga
     5521 cgactgcgac attattatta ttggtgccgg tattgcaggc accgcttgcg cgttacgctg
     5581 cgcgcgagcg ggtttatccg ttttgttact ggaacgcgct gaaatccccg gcagcaaaaa
     5641 tctttccggc gggcggttat atacccatgc actcgcggaa ctcctccctc agtttcatct
     5701 gaccgcgcct cttgaacgac gcatcactca cgaaagcctt tccctgttaa cgccggatgg
     5761 cgcaacgacg ttttccagct tacagcccgg cggtgaatcc tggagtgtat tacgtgcgcg
     5821 gttcgatccg tggctggttg tcgaagccga aaaagaaggt gtcgaatgca tccctggtgc
     5881 gacggtggat gcactgtatg aagaaaacgg cagggtgtgt ggtgtcattt gtggtgacga
     5941 tattctccgc gcccgttatg tggtgctggc agaaggtgcc aacagcgtcc tggctgaacg
     6001 tcacggatta gtgactcgtc ctgctggcga agcgatggcg ttggggatca aagaagtgct
     6061 gtcgctggaa acatccgcta ttgaagaacg ttttcatctg gagaataacg aaggcgcagc
     6121 gttgctgttc agcggcggga tctgtgatga cttacccggc ggcgcatttc tttatactaa
     6181 tcaacaaacg ctctcgttag ggattgtttg cccgctctct tcccttacgc aaagtcgtgt
     6241 tccggcaagc gagctgctgg ctcgctttaa aacgtatccg gcagtgcgcc cgcttatcaa
     6301 aaacacggaa tcactggagt atggtgcgca tctggtgcca gaaggtggct tgcacagtat
     6361 gccggtgcaa tacgccggta acggctggct gctggtgggc gatgcgttgc gcagttgcgt
     6421 caataccgga atttccgtgc gcggcatgga tatggcgctg actggcgcgc aggcggcggc
     6481 acaaacgctg ataagcgcct gccagcaccg cgagccgcaa aatctgtttg cgctttatca
     6541 tcacaacgtc gagcgcagcc tgctgtggga tgttctacaa cgttatcagc atgttccggc
     6601 gcttttgcaa cgcccgggat ggtaccgtgc gtggcctgcg ttaatgcagg atatttcccg
     6661 cgatttatgg gatcagggtg ataaacctgt tccaccgctg cgccagttat tctggcgtca
     6721 tttacgtcgt cacggcctgt ggcatctggc gggcgatgtt atcaggagtc tgcgatgtct
     6781 gtagcccgta atctctggcg cgttgctgat gcgccgcaca ttgttccggc tgactccgtt
     6841 gagcgccaga cggtacaacg gttgattaac gcttgtccgg ctggtctttt ttcgctcaca
     6901 ccgggaggtg acttacgcgt tgattatcac ggctgcctgg agtgtggcac ctgccgtttg
     6961 ctgtgcgacg aatcaacact acaacagtgg cgctatccgc cttccggatt cggcatcacc
     7021 taccgttttg gataaataat aaggataatt tatgcccctc ttacacctgc tccgccagaa
     7081 tccggtgatt gctgccgtta aagataatgc cagcctgcaa ctggcaatcg attctgaatg
     7141 ccaatttatt tccgtgttgt acggcaatat ctgcaccatc agtaatatcg ttaaaaagat
     7201 taagaacgcc gggaaatatg cttttattca tgttgatttg ctggaaggcg cgtcaaataa
     7261 agaagtcgtt attcagtttt tgaaactggt gaccgaggcg gacggcatta tcagtaccaa
     7321 agcctcaatg ttgaaagcgg caagagcgga aggttttttc tgtattcatc gcctgtttat
     7381 tgttgattcg atttcgtttc acaacattga taagcaagtt gcgcaatcga atccggattg
     7441 tattgagatc ctgccaggct gcatgcccaa agtgctgggc tgggtgacgg aaaaaatccg
     7501 ccaaccgctg attgctggcg ggctggtatg cgatgaagaa gatgcgcgta atgcgattaa
     7561 cgcgggtgtc gtggcgcttt ccaccacgaa tactggggtc tggacgttag cgaaaaaatt
     7621 gctttgacgg aataatcgta acgaattgaa tttgtgtcat ttgtaggctg cacgccacat
     7681 ccgacattca gcgcctgatg cgacgcttga cgcgtcttat caggcctaca agtccccagg
     7741 ccagaaccgt aggcaggatc atccggcaac acgttattgg caatctgcgt gaatgtttgt
     7801 caccagtgcc tccagcacca ctttccagtc atcaaccacg ccaacatctg cctgtgaaaa
     7861 taccgctgcg ctggcgtcat gattaatcgc caccacaaac ttgctattgc atacgcccgc
     7921 cattaacgct gctgcgccag aagcgccaac aacaatacac acctcaggtg ccagcagatg
     7981 tccggaaata ccaatcactt tttcagcatc aacgccaccg ttcatcactc gcgcccgact
     8041 gtagcccact tcggctccca gcttttctgc cagcatagcg atttcctggt tatctgcctc
     8101 tccgccctgc cccaccacca gcacccatct ggcctcagct aatgggtcgc gagtaacatt
     8161 tttcagatcc tctgtgctaa ccagccagtc gggaagcgca cctgggacta tgttcagttg
     8221 ctgcatacca gagggcaacg tggcattttt atctgttccc gcctggcgcg ccagggaaag
     8281 acatagcggg cgtttttctg tttgcagcgt cgccgttagc gcatttcccc agtgggattt
     8341 gcgcacgctt acggttggaa tatctaacga agtcacctgg cagatgcttg caccatgtaa
     8401 acgccaggcc agccgggtcg acagttcatc gccaaacgtt cccggcggaa agagaacaac
     8461 gttcgcaggc gttcgctgcc actgttccac cagcgcatcc agcacctgtt ccaccactac
     8521 gggctgtggt tctatttgcc agtgcgccag ggtacaaccg ctgaaatcct gcgccgccag
     8581 ccagcccgct aatgcggcat tctcctgatt tattgtcaca attgcgatat tcatggctgc
     8641 atcctttggc gtaaatagtc ctgccatagc ttttgcgctt tttctgctac cgtttgccca
     8701 tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca gttgcagaca ctgcatcgct
     8761 ggcaattcgg cagcaacagt tttacggata atttccgctt tccccgcagc cattcgctgg
     8821 cgcattcccg gtactggcaa ggccacttca ccgcactggc gcacggcaat caccgcaggc
     8881 aagcgaaccc aacaacagcg cagcccatgt tcagtacgct gttcgagggt gataaacagc
     8941 gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc agcccagcat ttccgccagc
     9001 aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc agccggtaat tatcagatcc
     9061 agcggattct gacgctgcca ctcggcaata tggcgagcga caaattccga cgcaaagcgc
     9121 agatctgccg ccgtctccag caatacagct tcctcaaacc caagagccat gagatagcgc
     9181 agccagtgta acgcccgttc atcccccatg ctcaacgcgg ttaaagacat cggcgtaccg
     9241 ttttttctct gcgccaacag cagcgcggcg gcagcctgtt catcagcacc gagtaaactt
     9301 cgcagtagcg aaacatccgg tccgctatta ccctgagccg ccgcctgcca ctctttttcc
     9361 gccagcattc cggcatccgg ttcggcttta aacgctaaca gaatgttcat tcgcacctcc
     9421 tacatttccc gccgccacca gcgggagggc tttggtttct ggtgcccata gccaggtcac
     9481 aacaaaacca accaacaaca ctgccgccag gagcaataag gtgacttgca ttccccactg
     9541 cgccagcacc cacggcagca ggccagtact taccgccgcc cccaaccgac tcatggcggt
     9601 ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa ctttccgcag gcaaaatgcc
     9661 caccagatta ctgactgccg aaatggtggt gctgaagaga acaaaaagca gtagcgttaa
     9721 tgaactgccg gaaggcaaac aggccatcac taccagcgtt gccgccagca gcaaaaaact
     9781 tcccagcaaa aacttgcgat gtgccagcag gtgcgtcaga actaatccca gcagcgcgcc
     9841 cacaattaac aacgcattaa gcatcaggct ggcagtcagc gcatcttcca gtccaatagt
     9901 ctgggcgata gttggcagcc aggtataaat cacaaaccat gggattacga ggcagacaaa
     9961 gaagacgctg ttaaacgccg tgcgccgcca gtagcgcgaa gagaacaagg ttttgatgtg
    10021 tttatgggtc gccgttgcca cttcatcgcc cagtaaaaca tggggaccaa aatagcgatg
    10081 cacgatagcg tgagcttctg caaaacgccc ctggcgcagt agccagcgtg gcgattctgg
    10141 ggttccccag cgtaataacg taatcaacaa cgcgggcaga gctgccgatg ccagcagcca
    10201 gcgccaggcc tccgggcttt cgctaataaa gtgatgtccg gcaatacttg ctaacacata
    10261 gcctacggtc cacaccacgc tgaatgcgcc cagcaaaaca ccgcgatggc ggcgcgggga
    10321 aaattcagcc agcaaggtgt gacctactga gtaatcgcct cccagaccaa tgccgatcaa
    10381 aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa aattgcaaga acgaagcgag
    10441 cgtaatcagc aaaaagctga aggtgaagat tttttgccga ccaatatggt cggagatcca
    10501 cccaagaacc aggctaccaa gaaacaaacc aaggagcgcc gagccgccga tcattcccgc
    10561 cataaacggc gtcagttgca tggcgggcgt aagctgaata atggcgtaac caatgacgcc
    10621 gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc gcagcaattc gacagtgaaa
    10681 acggttaagc ggtaaatcat ccattcgcac cggtgaagtg ttcatcttta ccccttcatt
    10741 gtccggtaat aagaacggct tctcgcctga gaagccgaaa aggcaacgcc ccatcatcgc
    10801 ggaacgctgc ctgacacatt atttttcaat cggatagata gtacccgtat tcataatgcc
    10861 gttaggatcg aattgctttt tcagcccttc cagcaacgcc cacgcgctgc cgtgttccag
    10921 cttgctccag tgaacgcgat gtttaccaat gccgtggtgg tgcaccatcg aaccgccgag
    10981 gcgaatggtt tcttcgcaga tgattttgtt gagcggattg tggtacttgt cgatttcctc
    11041 ttccggctta cagtcaacaa cgttgtagtc atagacgaag tacatgtttg tgccgttctg
    11101 atagctatga gaggagtgac cgcccagcat ggtgatgtcg tcggcgtgcg ggaactcggt
    11161 acgaatacgg ttaataacac tttcgtagat ttcgtgaatg cagctccagc agccggatac
    11221 ttcggtggta aagcccatgt tgccggtttt gaggatctgc acacgttcgg cagccacttt
    11281 atccggtccc cagttcaggt tgttaaacca ggtttcgatc agcttgctgt ccacgcgctg
    11341 gcattgcggg tagcgggcaa cgatttccgc aatcccttcg cccgtcgcct tcgcaatgcg
    11401 agggttacct tcagccataa agatcagcac gcattttccg tcggcaaaat gggtgaagtg
    11461 ttgggtgcca tcttcagcgt catacaaacg agcgatcgac ggacgatacc cttccaccat
    11521 gatttcacgc aggatgttga agccggtttt catgtcttcc aggatatagc cgtagaagag
    11581 gttgttttcc ggggtgaatt taaagatttt cactgttact tcagtgatat agcacaatgc
    11641 accttcgttg ccgatgatga tgtgacgaat gtccgggcca gccgcgcggc gtggcacgtt
    11701 tttaatacgt gtgacggtgc catcagccaa tacggcttcc agaccaacga ccatatcttc
    11761 gattgcgccg tagagtgtgg agaactgccc gatactgcgg gttgctacca ggccgcccat
    11821 ctgcgccagc ggctttgact gcggagaatg ccccgtggtg taaccttttt cacgcaacgc
    11881 gttttccagc acctccagcg gaacgccaca ttgcgccgtc gcctgcatat tctcaatatc
    11941 aatattaatg atttgattca tggcggaacc gtcgagcacc accgagtttt ctacaacagt
    12001 ttccagccca ccttcggtgg cggaagcacc ggtacgcggc acaccgttaa ttttgtgcgc
    12061 attcataaaa ttcagcacac gggagacttg ttctgtggaa ccgagtttta cgaccgcagc
    12121 cggaatcggc aaagtataaa taccatgaat atccggaaat ttacgaaaac ggtcaatact
    12181 gttcttcttt aatactgttt catcggtaat tacgcgatct gcaccaacaa tttcctttag
    12241 ctggtcgaca atcgctgcgc gagataaaga catagtaatt ccttcctgat aataaaaatg
    12301 gaataatcat taataagaga aaacagaaac caccgttatg ggttagcgca ctaaataacc
    12361 gccatcgacc actaataaat gcccgttgac ataattcgat gcctgacttg cgaggaatac
    12421 ggctgcgccc attaaatctt gagtatcgcc ccaacggttt gccggaatat gatcaagaac
    12481 gcgctgattg gtttctggat tactgcgtgt cgccagcgta atatctgttg cataatagcc
    12541 aggggcgata ccatttacct gaatattata ttgccctaat tcatcacaat aagctttggt
    12601 gaatccggca agagcatgtt tagtggcaga atatgcgggt gaccattgtc cacctaagta
    12661 agagaacaat gaacagatat taataatttt accgcttttc tgcgggatca taatttttgc
    12721 cgcttcatag cttaactcga atgcggcggt caggttcaca tcaatcatcg ggtcccagtc
    12781 ggcacgaccg aagtccagca ccttattcag cttacaaata ccggcattgt taaccagaat
    12841 atcaactgta ccgaaacgct cacagcaggc agcgataatc ttctgcggcg cgccttctgc
    12901 ggtgatatcc acctgcatga agtccacctc aacaccctga ttttcaatca ttttctttgt
    12961 ttcgccgtta tctttgacaa aacttggaat aaagacattt gcgcctgctt tggccaacgc
    13021 catggcaaat gcctggccta aaccgctatt cccgccggta acaattgcgg ttttaccttt
    13081 cagggagaaa aaatccattg agaacgcatt gagagattcg attgacatag ttagctccaa
    13141 tttccgtaag gcaaaaaaaa agaaaggtaa cttccccgcg agagggaagt tacctttctc
    13201 attatctctg gtaactggag ataattttta gcataaggac ataactgcta atgtgatcgt
    13261 aatcacagtg tgatattcgc attatcaaat agttttaaaa agcagaagcg tgattactct
    13321 cacacaacac ctctgctttc catgttattt attatttcaa ttact
//
LOCUS       DAZUCE010000130        13343 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZUCE010000130 DAZUCE010000000
VERSION     DAZUCE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.130"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(716..1807)
                     /gene="ychF"
                     /locus_tag="ACHY03_003243"
     CDS             complement(716..1807)
                     /gene="ychF"
                     /locus_tag="ACHY03_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFS3906437.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1924..2508)
                     /gene="pth"
                     /locus_tag="ACHY03_003244"
     CDS             complement(1924..2508)
                     /gene="pth"
                     /locus_tag="ACHY03_003244"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFS3906438.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2786..3064
                     /gene="ychH"
                     /locus_tag="ACHY03_003245"
     CDS             2786..3064
                     /gene="ychH"
                     /locus_tag="ACHY03_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFS3906439.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3119..4798)
                     /gene="dauA"
                     /locus_tag="ACHY03_003246"
     CDS             complement(3119..4798)
                     /gene="dauA"
                     /locus_tag="ACHY03_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFS3906440.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4923..5870)
                     /gene="prs"
                     /locus_tag="ACHY03_003247"
     CDS             complement(4923..5870)
                     /gene="prs"
                     /locus_tag="ACHY03_003247"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFS3906441.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6021..6872)
                     /gene="ispE"
                     /locus_tag="ACHY03_003248"
     CDS             complement(6021..6872)
                     /gene="ispE"
                     /locus_tag="ACHY03_003248"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFS3906442.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTTADSGRLPMGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6872..7495)
                     /gene="lolB"
                     /locus_tag="ACHY03_003249"
     CDS             complement(6872..7495)
                     /gene="lolB"
                     /locus_tag="ACHY03_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFS3906443.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7709..8965
                     /gene="hemA"
                     /locus_tag="ACHY03_003250"
     CDS             7709..8965
                     /gene="hemA"
                     /locus_tag="ACHY03_003250"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFS3906444.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9007..10089
                     /gene="prfA"
                     /locus_tag="ACHY03_003251"
     CDS             9007..10089
                     /gene="prfA"
                     /locus_tag="ACHY03_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFS3906445.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10089..10922
                     /gene="prmC"
                     /locus_tag="ACHY03_003252"
     CDS             10089..10922
                     /gene="prmC"
                     /locus_tag="ACHY03_003252"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFS3906446.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10919..11311
                     /gene="sirB2"
                     /locus_tag="ACHY03_003253"
     CDS             10919..11311
                     /gene="sirB2"
                     /locus_tag="ACHY03_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFS3906447.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11315..12124
                     /gene="sirB1"
                     /locus_tag="ACHY03_003254"
     CDS             11315..12124
                     /gene="sirB1"
                     /locus_tag="ACHY03_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFS3906448.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12160..13014
                     /gene="kdsA"
                     /locus_tag="ACHY03_003255"
     CDS             12160..13014
                     /gene="kdsA"
                     /locus_tag="ACHY03_003255"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFS3906449.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13162..13269)
                     /gene="ldrA"
                     /locus_tag="ACHY03_003256"
     CDS             complement(13162..13269)
                     /gene="ldrA"
                     /locus_tag="ACHY03_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS3906450.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3348 a         3343 c         3426 g         3226 t
ORIGIN      
        1 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
       61 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      121 ctatgaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt
      181 tgtaataatc ctaattagtt acgggatttt cgcttgatgt atctgcataa caaaaacaat
      241 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
      301 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      361 ggatcgcacg taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa
      421 gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggctg
      481 atgtgtagtg gaacaaaaac tgacgttata tcactgctaa tgagatatcc gtcatctgaa
      541 cacttatgtc tttaattatt ctcgtggttc actgtaggca ataagcacaa aagtgtagga
      601 tgttacaaga atgattcgga ctcggtgaaa tgaaaaatcc acgcaattgc gtggatttta
      661 tatacttttg cgctcttcat gagatttagc gaaacctcat gagacaataa attaattaga
      721 cgttgaaaag gaagttcatc acatcgccat ctttcacgat gtaatcttta ccttctgcac
      781 gcattttgcc tgcttctttc gcgccttgtt cacctttgta agtgatgaaa tcttcaaacg
      841 agatggtttg tgcacggata aagccttttt caaaatcagt atggattttg cccgctgcct
      901 gcggcgcggt tgctccaacc ggaatggtcc atgcacgcac ttctttcacc ccagcggtga
      961 agtaagtttg caggttcagc agcttataac cggcacggat cacacggttc aggcccggct
     1021 cttccagccc aagctcctgc ataaactcgt cacgttcttc gtcgtccagt tcggcaatgt
     1081 ctgcttcaac agcagcacaa accggaacca caacagaacc ttctttcgcc gcgatttcac
     1141 gcacctggtc aagatatggg ttgttttcaa aaccgtcttc gttgacgttg gcgatgtaca
     1201 ttgttggttt cagcgtcagg aagctcaggt aacgaatagc cgctttctct tcagcgctta
     1261 aatccagcgc gcgcagcata cctgcgtttt ccagctgggg caggcatttt tccaggaccg
     1321 ccagctcagc tttcgcgtct ttatcgccac ctttggcttt cttctgtacg cgatgaatcg
     1381 cacgttcgca ggtgtcgagg tctgccagcg ccagttcggt gttgataact tcaatatcgt
     1441 cagccgggtt aactttgccg gaaacgtgaa tgatgttgtc attttcaaag cagcgaacaa
     1501 cgtgaccgat cgcttcggtt tcacggatgt tggtcaggaa ctggttaccc agaccttcgc
     1561 ctttcgatgc gccttttacc agaccggcga tatcgacaaa ttccatggtc gtgggaagcg
     1621 tacgttgcgg ttttacgatt tcagccagtt gatccaggcg aggatcaggc attggtacga
     1681 cgcctgtgtt cggctcaatg gtgcagaatg gaaagttggc cgcttcaata ccggctttgg
     1741 tcagcgcgtt gaacagggta gatttcccga cgttgggcaa accgacgata ccgcatttga
     1801 atcccatgat ttaaatcacc ttaatatctt aataatcaac ctgttattgc taacagattg
     1861 cagaaatgga aataactttg cctattatac acggcactcg gcaaaaatgc cgcagacaac
     1921 gacttattgc gctttaaagg cgtgcaatcg gttcgttgct ttggtcaagc catctgtaaa
     1981 ccacatttca gtacaacgcg ccgcttcgtc gatggcttca tcaattaact tctgttcact
     2041 aacaggcggt ttgcctaaca caaaaccaac aactttattt ttatcgcccg gatgaccgat
     2101 tccgatgcgt aaacggtgaa agttagggtt attacccaat ttactgatga tgtctttcag
     2161 tccattgtga ccaccatggc caccgcccaa cttaaatttg gcgacgccag gaggcagatc
     2221 cagttcgtcg tgggccacca gaatttcgtc cggattaatg cggaaaaaac tggccatcgc
     2281 cgcaacggct ttgccgctga gattcataaa tgtagtcggg actaacaggc ggacatcttc
     2341 gcctccaagg gtgactcgcg aagtataacc aaagaattta gcctcttcgc gcagcggagc
     2401 gcgcaaacgc tctgccagta agtcaacgaa ccaggcacca gcattatgtc gcgttgcggc
     2461 gtattcagca ccggggttcg ccaggccgac aatcaattta atcgtcacgt ttttttgtcc
     2521 tgagtgtgta cataactggc gcgtagttta ctggttgcgg ccccgcttga caaaaaactg
     2581 cgtatcaaat gcagataacg taataattgc ctgagtggac tattagaaag tcagggtgtt
     2641 caggcgttta tttgtaaagt ttttttgaaa taagggttgt aattgtgatc acgcccgcac
     2701 ataacccaca gggtgttgtc tatactttac acataaggaa gaggggtatt ccctgttaca
     2761 acccagaaag ttccggaggt gacatatgaa acgcaaaaac gcttcgttac tcggtaacgt
     2821 gctcatgggg ttgggtctgg tggtaatggt ggtcggcgtg gggtattcaa tcctcaacca
     2881 gttaccacag tttaatatgc cccagtattt cgcacatggt gcagtgctaa gtattttcgt
     2941 cggtgccatt ctctggctgg cgggtgcccg tgttggcggg catgaacagg tgtgcgaccg
     3001 ttactggtgg gttcgccact atgacaaacg ttgccgccgt agcgataatc gccgtcatag
     3061 ctaacaaata atgcagtttg ctgaccagtc agtttgcgct gactggtcaa tctcgtactt
     3121 ataaatccgc catcgccgcg cgacgattcg ggaagaacgc gaggcgtccc gggatcggtt
     3181 gaatgcctgc gcgagccata gtgcgcagtg gctggaattc cacgttgcac acgcgcagtt
     3241 cacatccttc gggcagacgc ttcacaaaac gctggaacgc atcaagacca ccagcatcaa
     3301 gtaccggaac ggcatcccac ttcagaatca caatccgttt gccttcaaga cgtgactcca
     3361 ggtccgtgaa taagccttca gcagcagcaa aaaacagcgg gccaataacg cgcagtacca
     3421 ggacatcgtc tggaacatct acgactaccg gtgccaggcg agtcatacgt gcgatacgac
     3481 gcataaacag cagcgatgcc agcacgatcc ccacgctgat ggcaataacc atatcaaaca
     3541 gcacggttag cgacatgcac agcagcatga caatgatgtc atctttcggt gcatgacgca
     3601 gcaagtcgac cactttatgc gcttcactca tgttccacgc caccatcaac agcagggctg
     3661 ccatagcgga aagcggcagc caggagagca gcggtgccag taccagcagg gcaagaataa
     3721 ccagaataga gtggatcacc gccgagatag gggaagttgc cccggcacgg acgttagcgg
     3781 cagaacgcgc gatggcagct gtagcggtaa taccaccaaa gaacggagcg atgatattcc
     3841 ccagtccctg tccaaccagt tcgctattcg ccttgtgttt cgtcccggtc ataccatcca
     3901 gcaccacggc gcagagcaga gattcgattg cgccgagcat tgccattgag aatgccgcag
     3961 gcagcagtgt gcgaatagaa tcccaggtta gcgtgaattc tgaattaggc agatcccacg
     4021 gcagcaccag ttgcggcagc agttgcggaa taccgttacc ctgagaacca tcggccagga
     4081 cgtagtggaa ttgcgaaccg atggtagcaa catgtccgcc gagcaggtta acaatcccca
     4141 tcaccgcgca accagccagc aaggccggaa ggtgaccggg taaacgaatg cccagacgcg
     4201 gccagaaaac aagaatacct agcgtcacaa tgccaatggc agcatcaccc acattaatgg
     4261 tcggcagcgc cataaataat gcgccgactt tttgtagata atgttccggg acatgggcca
     4321 tttgcagacc gagaaaatct ttaatctgca tggtaccgat ggtgatcccg atacccgagg
     4381 tgaaacctaa ggtgacggaa accggaatat actcaatcag gcgaccaaag cgtgccagac
     4441 ccatcagaat caaaaagatc cccgacagca aggtcgcaac cagcagtcct gccagtccaa
     4501 actgttgcga cacgggatag agaattacca caaatgccgc agtcggaccg gaaacgctaa
     4561 agcgtgaccc acccgtcaga gcaatgacaa tccccgcaac agctgcggta tataaaccgt
     4621 actggggtgc cacaccacta ccaatagcca acgccatcgc cagcgggata gcaataatcc
     4681 cgacggttat cccggcaatc aggtcacggg taaaccgtgc ggcagtatat ttttctttcc
     4741 agcaagcgtc gatcagagcg cggaaaggca tcacatgtga ggaaaatatt ttgttcacaa
     4801 taatgtttca tccgtgagcg catcatctgt caactaaatg gcaggtgaag gaggcatagg
     4861 tcatacaaat ggatattaca gacaaaaaaa cccgccgcag cgggtctttg agccgggttc
     4921 gattagtgtt cgaacatggc agagatcgat tcttcgttgc tgatacgacg aatcgcttcg
     4981 gccagcatac ctgacagggt cagagtacgc acgttcggca gtgatttgat ttcatcgctc
     5041 agcggaatgg tatcgcagac aacgacttca tcaattacag agttacgcag gttgttcgcc
     5101 gcgttgccag agaagatcgg gtgagtcgcg tacgcaaata cacgtttagc accacgttct
     5161 ttcagagctt cagcagcttt acacagcgta ccgccagtgt cgatcatatc atcgaccagt
     5221 acgcagtcac gacctgcaac gtcaccgatg atatgcatca cctgggaaac gttcgcacgc
     5281 ggacgacgtt tgtcgatgat tgccatatcg gtatcgttca gcagcttagc gatagcgcgg
     5341 gcacgcacaa cgccgccgat gtccggagaa accacaattg ggttatccag attcagctgc
     5401 agcatgtctt ccagcaggat cgggctacca aatacgttat caaccggaac gtcgaagaaa
     5461 ccctgaatct gttcagcgtg cagatccact gtcagcacac ggtcaacacc gacgctggag
     5521 aggaagtctg caaccacttt cgcagtgatt ggtacacgag cggaacggac gcgacggtcc
     5581 tggcgcgcat agccaaagta ggggataaca gcggtgatac gacctgcgga agcacgacgc
     5641 agggcatcaa ccataacgac taattccatc aggttgtcgt tagtaggggc acaagtggac
     5701 tggatgatga aaatatcacc accgcgtaca ttttcattaa tttgtacgct gacttcgcca
     5761 tcgctaaagc gacctacagc ggcgtcgccg agtgaagtgt acaggcggtt ggcaatacgt
     5821 tgtgctagtt ccggggtggc gttaccagca aaaagcttca tatcaggcac gagaagaacc
     5881 tcaggcatgc gtccattggt ggaaagaatc tgccgaaaac tgtgcgggcc aggcatgatc
     5941 ctttccaggc ggtgtattaa agagcgcgat gcaacgtctg gaacaaggtg acgttgtcac
     6001 cgaaactcag cttgcccggc ttaaagcatg gctctgtgca atggggaaag attagcgcct
     6061 ttcgccacaa agccattgag ccattccggg gcttgctcta gcacctggcg ggcttcagac
     6121 tctgtatcaa attcagcaaa gacacaggcc cctgtcccag tcaggcgcga cggggcgtat
     6181 tctaacagcc aggaaagcac cgcatcaacc tcgcgaaaac gttttcttgc gataacctcg
     6241 caatcattgc tgaattcaca ttttagcaac gtttctattg acctttttgg cgtattgcgc
     6301 gggagttcag gatctttaaa aatcaccgga gtcggaatac ttacaccagg gtgcgccacc
     6361 agataccact tctctggcgg atccaccggc gttagtattt caccaacgcc ttcggcaaac
     6421 gcggcatgcc cccgaacaaa gacaggaaca tctgcgccca gcgtcagccc catttccgcc
     6481 agctcatcca tgcttagccc gcattgccag agatgattta acgccaccag aaccgtcgcg
     6541 gcattggatg aaccaccgcc gagaccgccg cccatcggca aacgcttgtc aatgctgata
     6601 ttcgcaccgc ttcccatcgg aagacgcccg ctgtctgccg tagttttcat caacaaccgc
     6661 gctgcgcgaa cgatcaggtt atcttcatgt tccacgcctt caacgggcgt taacagacga
     6721 atatccccat cgtcacgaag ctcaatgctg atggtgtcgc cgtaatcaag aaactgaaac
     6781 agcgtttgca gcgtgtggta accatccgca cgctgaccgg taatgtataa aaacagatta
     6841 agttttgccg gagagggcca ctgtgtccgc attatttcac tatccagtta tccattttta
     6901 acttgatgcg ttgaccaccg tcggtgagtt ccatattggc tggcatcgca ggttgcgttt
     6961 tggtgtcata accaccataa acaaccttcc agtttttgcc attctggctg taggtaattt
     7021 cgctcaggcg gtactggtcg tccagtttgt agtcggttgc atcacccggt aaacctaaaa
     7081 tccactggcg caagctgttg agcggaattg gcattccagt caatttgcca atcatctctt
     7141 cggcgtcatc ggcggtataa cgctgacctt tattgtcgac taactgcacg ttacccggtt
     7201 gagcattcag ctccagttcc gtactgccca atgggttagt cagcagcaga cggtagcgat
     7261 cctgaccagt ttgctgccag aagaaacggg cgtacacttt ttgttggtca gaaatataag
     7321 cgaacgcgcc gcgagtctga tactgattaa gattgcgcac gtcttgctga tgctgacgcc
     7381 attgtggcga atccgggctt ttgccaggac ctttgggcgt ggtaacggaa caggcagtga
     7441 gcacaagagc agccagcggt agcaggcgga taagacgaaa atcgggcagg ggcatagtga
     7501 tgacaagtcc ttgagatacg ttgcagttat aacccttaat gctagcgtca ccattagtca
     7561 tcgtctatgt tcaagttgtc ttaattgtag aaatctaacg ggttcgggca attactccaa
     7621 aagggggcgc tcccttttat tgatctcgcg catacggtat gatgcgccga gactaacctt
     7681 atcaacgttg gtattatttc ccgcagacat gaccctttta gcgctcggta ttaaccataa
     7741 aacggcacct gtatcgctgc gagaacgtgt atcgttttcg ccggacaaac ttgatcaggc
     7801 gctggacagt ctgctcgcac agccgatggt gcagggcggg gtggtgctgt cgacgtgtaa
     7861 ccgcactgag ctttatctta gcgtcgaaga gcaggacaac ttgcaagagg cgttaatccg
     7921 ctggctttgc gattatcaca atcttaatga agaagatctg cgtaaaagcc tctactggca
     7981 tcaggataac gacgcggtta gccatttaat gcgtgttgcc agcggcctgg attcactggt
     8041 tcttggggag ccgcagatcc tcggtcaggt taaaaaagcg tttgccgatt cgcaaaaagg
     8101 ccatatgaag gccagcgaac tggaacgcat gttccagaaa tctttctctg tagcgaaacg
     8161 cgttcgcact gaaacagata tcggtgccag cgctgtgtct gtcgcttttg cggcttgtac
     8221 gctggcgcgg cagatctttg aatcgctctc tacggtcaca gtgttgctgg taggcgcggg
     8281 cgaaaccatc gagctggtag cgcgtcatct gcgcgaacat aaagtacaga agatgattat
     8341 cgccaaccgc actcgcgaac gtgcccaaat tctggcagat gaagtcggcg cggaagtgat
     8401 tgccctgagt gatatcgacg aacgtctgcg cgaagccgat atcatcatca gttccaccgc
     8461 cagcccgtta ccgattatcg ggaaaggcat ggtggagcgc gcattaaaaa gccgtcgcaa
     8521 ccaaccaatg ctgttggtgg atattgccgt tccgcgcgat gttgagccgg aagttggcaa
     8581 actggcgaat gcttatcttt atagcgttga tgatctgcaa agcatcattt cacacaacct
     8641 ggcgcaacgt aaagccgcag cggttgaggc ggaaactatt gtcgctcagg aaaccagcga
     8701 atttatggcg tggctgcgag cacaaagcgc cagcgaaacc attcgcgagt atcgcagcca
     8761 ggcagagcac gttcgcgatg agttaaccgc caaagcgtta gcggcccttg agcagggcgg
     8821 cgacgcgcaa gccattatgc aggatctggc atggaaactg actaaccgct tgatccatgc
     8881 gccaacgaaa tcacttcaac aggccgcccg tgacggggat aacgaacgcc tgaatattct
     8941 gcgcgacagc ctcgggctgg agtagcagta catcattttc tttttttaca gggtgcattt
     9001 acgcctatga agccttctat cgttgccaaa ctggaagccc tgcatgaacg ccatgaagaa
     9061 gttcaggcgt tgctgggtga cgcgcaaact atcgccgacc aggaacgttt tcgcgcatta
     9121 tcacgcgaat atgcgcagtt aagtgatgtt tcgcgctgtt ttaccgactg gcaacaggtt
     9181 caggaagata tcgaaaccgc acagatgatg ctcgatgatc ctgaaatgcg tgagatggcg
     9241 caggatgaac tgcgcgaagc taaagaaaaa agcgagcaac tggaacagca attacaggtt
     9301 ctgttactgc caaaagatcc tgatgacgaa cgtaacgcct tcctcgaagt ccgagccgga
     9361 accggcggcg acgaagcggc gctgttcgcg ggcgatctgt tccgtatgta cagccgttat
     9421 gccgaagccc gccgctggcg ggtagaaatc atgagcgcca gcgagggtga acatggtggt
     9481 tataaagaga tcatcgccaa aattagcggt gatggtgtat atggtcgttt gaaattcgaa
     9541 tctggcggtc atcgcgtgca gcgtgttcct gctacggaat cgcagggtcg tattcatact
     9601 tctgcttgta ccgttgcggt aatgccagaa ctgcctgacg cagaactgcc ggacatcaac
     9661 ccagcagatt tacgcattga tactttccgc tcgtcagggg cgggtggtca gcacgttaac
     9721 accaccgatt cggcaattcg tattactcac ttgccgaccg ggattgttgt tgaatgtcag
     9781 gacgaacgtt cacaacataa aaacaaagct aaagcacttt ctgtactcgg tgctcgcatc
     9841 cacgctgctg aaatggcaaa acggcaacag gccgaagcgt ctacccgtcg taacctgctg
     9901 gggagtggcg atcgcagcga ccgtaaccgt acttacaatt tcccgcaggg gcgcgttacc
     9961 gatcaccgca tcaacctgac gctctaccgc ctggatgaag tgatggaagg taagctggat
    10021 atgctgattg aaccgattat ccaggaacat caggccgacc aactggcggc gttgtccgag
    10081 caggaataat ggaatatcaa cactggttac gtgaagcaat aagccaactt caggcgagcg
    10141 aaagcccgcg gcgtgatgct gaaatcctgc tggagcatgt taccggcaga gggcgtactt
    10201 ttattctcgc ctttggtgaa acgcagctga ctgacgaaca atgtcagcaa cttgatgcgc
    10261 tactgacacg tcgtcgcgat ggtgaaccca ttgctcattt aaccggggtg cgagaattct
    10321 ggtcgttgcc gttatttgtt tcgccagcga ccttaattcc gcgcccggat acggagtgtc
    10381 tggtggagca ggcactggcg cggttgcctg aacaaccttg ccgtattctc gatctcggga
    10441 cgggtaccgg ggcgattgcg cttgcgctgg ctagcgagcg cccggactgc gaaattatcg
    10501 ctgtagatcg tatgcctgat gctgtctccc tggcacaacg taatgcccag catctggcga
    10561 tcaaaaatat ccacattctg caaagtgact ggtttagcgc gctagccggg cagcagtttg
    10621 cgatgattgt cagcaatccg ccgtatattg acgagcagga cccacatctt caacaaggcg
    10681 atgtccgctt tgagccgctc actgcgctgg ttgcggcaga cagtggaatg gcagacatcg
    10741 tgcatatcat cgaacagtcg cgtaacgcgc tggtatccgg cggctttctg cttctggaac
    10801 atggctggca gcagggcgaa gcggtgcgac aggcatttat cctcgcgggg tatcatgacg
    10861 tcgaaacctg ccgtgactat ggtgataacg agcgcgtaac gctcggccgc tattatcaat
    10921 gacaagtttt tctacactgc ttagtgttca tcttattagt atcgcgcttt ctgttgggct
    10981 attaacctta cgtttctggc tacgttatca gaagcatcca caggcatttg ctcgctggac
    11041 gcgcattgtg ccgccggttg tcgatacggt gttattgtta agcggcattg cgttgatggc
    11101 taaagcgcac atcctgccat tttccgggca ggcacagtgg ctgactgaaa agctgtttgg
    11161 agttatcatt tatatcgttt tgggttttat tgcactcgat tatcgtcgta tgcacagtca
    11221 gcaggcgcgc attattgcct tcccgctggc gttggtggtg ctgtacatca tcattaaact
    11281 cgccaccaca aaagtaccgt tactggggta agtcatgaga tcgttagctg atttcgaatt
    11341 taataaagcg ccattgtgcg aaggcatgat cctggcttgc gaagcaatcc gccgcgattt
    11401 tccctcgcaa gatgtttacg acgaactgga gcgtctcgtt agtctggcga aggaagaaat
    11461 cagccagctt ctgcctttag aagagcagct ggaaaaactg atcgcgctgt tttacggcga
    11521 ctggggattt aaagcctcac gcggcgttta tcgtctttcc gatgcattat ggctggacca
    11581 ggtgttaaag aatcgacagg gcagtgcggt atcattaggt gcggttttat tatgggtcgc
    11641 gaatcgtcta gatttgccgc tgctgccggt gattttccct acgcagctga tattgcgcat
    11701 tgaatgtccg gatggcgaaa tttggctgat taatcctttt aacggtgaat cgttaagcga
    11761 acatatgctg gacgtatggt taaagggaaa tatcagcccg tcggcggaac tgttttatga
    11821 agaccttgat gaagctgata acattgaggt aatccgcaaa ttgctggata cactcaaagc
    11881 ctcgttgatg gaagaaaatc agatggagct ggcgttacgc accagcgaag ctttattaca
    11941 attcaaccct gaagatccct atgaaattcg cgatcgcggg ttgatttatg cgcaactgga
    12001 ttgcgaacac gttgcgttga acgatttaag ttatttcgtt gaacaatgtc cggaagaccc
    12061 gatcagcgaa atgatccgtg cgcaaataaa taacatcgcg cataaacata ttgtgctgca
    12121 ttaattaatc gacattttac tcaagattaa ggcgatccta tgaaacaaaa agtggttagc
    12181 attggcgaca tcaacgtagc aaatgacctg ccgttcgtac tgtttggcgg tatgaacgtg
    12241 ctggaatctc gcgatctggc gatgcgcatt tgcgagcact acgtaaccgt gacccagaaa
    12301 ctgggtatcc cttacgtgtt caaagcctct tttgacaaag ccaaccgctc ctccatccac
    12361 tcttatcgtg gaccgggcct ggaagaaggg atgaaaatct tccaggagtt gaagcagact
    12421 tttggcgtga aaattatcac cgacgttcac gaaccaagtc aggcacagcc cgttgctgat
    12481 gtcgtggatg tgattcagtt gccggcgttt cttgctcgcc agactgacct ggttgaagcc
    12541 atggcgaaaa ccggtgcggt aattaacgtc aagaaaccac agtttgtcag cccgggacag
    12601 atgggtaata tcgttgataa attcaaagaa ggcggcaacg aaaaagtgat tctttgcgat
    12661 cgtggtgcta acttcggcta tgacaacctg gttgtcgata tgctgggctt cagcattatg
    12721 aagaaagtgt ctggtaactc gccggtgatt ttcgacgtga cccacgcatt gcaatgccgc
    12781 gatccttttg gcgcagcttc cggcggtcgt cgtgctcagg tggctgagct ggcacgagcc
    12841 ggtatggcgg taggtctggc ggggctgttt attgaagcgc atccggatcc ggaacatgcg
    12901 aaatgtgatg gtccatccgc gctgccgctg gctaaactgg aaccgttcct caagcagatg
    12961 aaagcgattg atgatctggt gaaaggtttc gaagaactgg ataccagcaa gtaatctttt
    13021 ttgcttgaaa aataaagtct tagcgttctg cgttaagact tttttcatgg gtgccggata
    13081 caaaaaaggc cgcaggctgt taccctgcgg ccggtttcgg gcgcatattg ccatcacggc
    13141 agcctgacgc ccgttttcgc cttacttccg gttacgccac cagccgacaa tcgctgcggt
    13201 aataattccc gccaggatcg gtgctgccag gtcgtgccag aaaatcatgg caaactgcgc
    13261 gagcgtcata tagccgcctt gttgtaatga caacattttg cggctattct tgaattgttg
    13321 atggttcaag attagccccc gtt
//
LOCUS       DAZUCE010000131        13313 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZUCE010000131 DAZUCE010000000
VERSION     DAZUCE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.131"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..813
                     /locus_tag="ACHY03_003257"
     CDS             <1..813
                     /locus_tag="ACHY03_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFS3906451.1"
                     /translation="NAANNQFNTNNHFLHHPKVDSELTRKFKIARMDTENIYLLPLTR
                     GNNHNYDGKSVVEIRKLDISKESWPFNYVTETCRESDGITTTGRMLYRNLKITSALDE
                     IYGGICKKAHAATELAEGLRLNLFMKSPSYPVEDYTVHEITLGPGCNVPGYAGTTIGY
                     ISTLPASQAKRWTNEQPRIDIYIDQIITVSGIANSSGFALAALLNANIEMGNDPIIGI
                     EAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK"
     gene            complement(954..1727)
                     /gene="uxuR"
                     /locus_tag="ACHY03_003258"
     CDS             complement(954..1727)
                     /gene="uxuR"
                     /locus_tag="ACHY03_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFS3906452.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHRDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1942..3402)
                     /locus_tag="ACHY03_003259"
     CDS             complement(1942..3402)
                     /locus_tag="ACHY03_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFS3906453.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3483..4667)
                     /gene="uxuA"
                     /locus_tag="ACHY03_003260"
     CDS             complement(3483..4667)
                     /gene="uxuA"
                     /locus_tag="ACHY03_003260"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFS3906454.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5007..6350
                     /gene="gntP"
                     /locus_tag="ACHY03_003261"
     CDS             5007..6350
                     /gene="gntP"
                     /locus_tag="ACHY03_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFS3906455.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6524..7426)
                     /gene="fimH"
                     /locus_tag="ACHY03_003262"
     CDS             complement(6524..7426)
                     /gene="fimH"
                     /locus_tag="ACHY03_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFS3906456.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7446..7949)
                     /gene="fimG"
                     /locus_tag="ACHY03_003263"
     CDS             complement(7446..7949)
                     /gene="fimG"
                     /locus_tag="ACHY03_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFS3906457.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7962..8492)
                     /gene="fimF"
                     /locus_tag="ACHY03_003264"
     CDS             complement(7962..8492)
                     /gene="fimF"
                     /locus_tag="ACHY03_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFS3906458.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNMLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8502..11138)
                     /gene="fimD"
                     /locus_tag="ACHY03_003265"
     CDS             complement(8502..11138)
                     /gene="fimD"
                     /locus_tag="ACHY03_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFS3906459.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTSWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11205..11930)
                     /gene="fimC"
                     /locus_tag="ACHY03_003266"
     CDS             complement(11205..11930)
                     /gene="fimC"
                     /locus_tag="ACHY03_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFS3906460.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11967..12506)
                     /locus_tag="ACHY03_003267"
     CDS             complement(11967..12506)
                     /locus_tag="ACHY03_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3906461.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVANGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12571..13119)
                     /gene="fimA"
                     /locus_tag="ACHY03_003268"
     CDS             complement(12571..13119)
                     /gene="fimA"
                     /locus_tag="ACHY03_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFS3906462.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLDQDGATSSAVGFNIQLNDCDTDVASKAAVAFLGTTID
                     TNHTNVLALQSSAAGSATNVGVQILDKTGAALALDGASFSAQTTLNNGTNTIPFQARY
                     YAIGAATPGAANADATFKVQYQ"
BASE COUNT         3233 a         3452 c         3207 g         3421 t
ORIGIN      
        1 aatgccgcta ataaccaatt caatacaaat aatcacttcc ttcatcatcc aaaagtcgac
       61 agtgagttaa cacgtaaatt taaaattgcc cgcatggata cggaaaatat ctatcttctg
      121 cctcttactc gtggcaataa ccataattac gatggaaaaa gtgttgtcga aattagaaaa
      181 cttgatatta gtaaagagtc ctggccattt aattatgtga cagaaacatg cagagagtcc
      241 gatggtatta caacaacagg acgtatgctc tatcgaaact tgaaaatcac ctcagcttta
      301 gacgaaatat atggtggtat atgtaaaaag gcgcatgctg ctacagagtt agcagaaggt
      361 ttacggctaa atttatttat gaagagccct tcctatcctg ttgaggacta tacagttcat
      421 gaaataacat taggccctgg ttgcaatgtt cctggttatg ccggaaccac gataggatat
      481 atttcaaccc ttccagcttc tcaggcaaaa agatggacca atgagcagcc aagaattgat
      541 atctatattg atcagataat aactgtctca gggatagcaa attcttccgg attcgccctt
      601 gccgcattac ttaatgctaa tattgagatg ggaaatgacc caatcattgg tatagaagcc
      661 tatcctggta ccgcggagat ccatgcaaaa atgggatata acgttatccc cggtgatgaa
      721 gatgcgccac tcaaacgcat gactctgcaa ccgtcatctc ttccagaact gtttgaatta
      781 aaaaatggtg aatggaatta tattggtaaa tagccactga gaagaatgag tactaatatt
      841 caggttatac agatatttaa acaataccct gcaatttaca aagtaatact cattgcgact
      901 tcttaattta agaaagcgca aggaacgttt acccttgcgc ttattataat aagtcaggcg
      961 tcgactttat ccagcggcca tgaatcaaac agatagccgt caaaataaat atcgtcaacg
     1021 ttcgagaatt ccagcagacg ttgcttaacg ttttccagat gctgccacat tgccagcttc
     1081 gccgcacggg catctttttt gattaacgcg gcgaggatct gtttgtgatc gcccaaccac
     1141 tctttgcgat acaggctgtc atcccgatgg ctgtgcaact ggatccacat tggattgttt
     1201 tcccgccact gccaggactg acggaataac tccaccagca tgctgttatg cgttgcttca
     1261 gcaatggcga gatggaactg catgtcaccg ctttcgctgc tacccggcgc gctggaagcc
     1321 agttcacgct cttccagttg caatgcctga cgcattttga cgatatcttc gcgggtagcc
     1381 tgcagggcgg caaactcggc gatgttactt tccagtaact gacgcgcctg taacagctca
     1441 aaaggacccg catcattgca gacgttggca tccggactgt ctgtgttgtg gctgcctgag
     1501 ctatcaagaa catagatacc ggcaccccgg cgcacttcca ccagcccttt gatctccagc
     1561 atgatcagcg cttcacgtac caccgtccgc gtgacatcaa gcatttctgc aatttcacgc
     1621 tccggcggta gccgttcacc aggattgtac ggcgtcttta tgatcagatc gcggatcatc
     1681 gccccgactt cctggtaagg tctttgcgca gaggtggcag atttcatggt gttgtgtccg
     1741 ttaggtgcgg ttaatcaaat ttttgccgga tgcggcgtga acgccttatc cggcctacaa
     1801 cgggcaattt tacggccccc gttaatctca aactatacca ttagcactgg aagttggcac
     1861 gtacaggtgg gcaaaaaaac gaaaactggt caaccaaata tcagtttgat tgaccagtaa
     1921 gtctgtaggg taatcagtta gttacagcgc agccacacac tcgcgcgcac cgcgttcgca
     1981 cagctgctga tatgccgccg tcaccgcgcc aacaaagtcg gcattttgcg gcagatcatc
     2041 ggcaaaaata ccgctcaggc ccagcagcgc tttcacgcgg tctgcgccct gatactgcgc
     2101 gttaatcttc tggaactcgg ccagcatcgg gtcgaccacg tcaatggcat taccctgctc
     2161 atccacgccc tgggtgtaac gcatccagcc agccacgccc agcgccaggt gacgccagct
     2221 gccgccgttt tgcaggtgca gacgcaccgg atccaacaga cgctgcggta acttctggct
     2281 gccgtccatc gcaatctgcc aggtacggtg acgcagagac gggttgctga aacgctcgat
     2341 cagcagcgtc gcataggcgt tcaggtccgt accttccggc atcgacagcg ttggcgcttg
     2401 ttcctgcatc atcagggcaa aggccgcttt gcgataatcc gggttagtca tggtgtcggc
     2461 aatagtttca tagccgccga ggtaaccgag gtatgccagg aaagagtggc tgccgttcag
     2521 catacgcagc ttcatcattt cgaacggcac aacgtcggca acgaactgtg cacccacttt
     2581 atcccagtcc gggcgaccat taacgaagtt atcttcaatc acccactgac ggaacggttc
     2641 gcaggcaatg gcgcatgggt cgtaaacgcc cagctggtca gcaatttcct gcaacgtttc
     2701 tggggtagcg gcaggaacga tacggtcaac catggtgcac gggaaggtga cgttctcttc
     2761 aatccatgcc gctagctgcg ggtcacgcgc ctgagccagc cccagtaccg cgaccttcgc
     2821 cacatgaccg ttttcacgca cgttatcgca ggacatcacc gtaaatgctt tcagcccttt
     2881 ttcacgacgc agacgcaagg cttcgacgat gtaaccaatc gcggacttcg gcgcagtcgg
     2941 gttttccaga tcgtgcttga tcagcgggtt attgagatcc agttgaccgc ttgccgcatc
     3001 agcgcagtag cctttttccg tgaccgttag agagacaatc gccgtttgcg gacgcgccat
     3061 cgcgttgaga ataccttcgc agccgtcgat ttccggatgc agcgcttctt tcatcgaacc
     3121 gataattttc agctcggtgc tttctgcgcc tttttccgct acggtgtaca gcagttgctg
     3181 ttttttcagg ttttcgatca gcacgcggtc gttgcctggc atcaggttaa cttcgcagat
     3241 gccccagtcg ctgtcggtgc tttccagcag atgatgggta tacagcgcct ggtgcgcgcg
     3301 gtgaaacgcc ccgcaaccga gatgcacaat gcgtgattcc agacgagaat gatcccatga
     3361 cgggcgggca accggcagat tgctgtcaac aatagtagtc atcgtaaact ccagaatccg
     3421 tcttcagaga gtgacggcaa ggaagaagga accggcacgg cgcgcagcca tgccggtgga
     3481 tattaacggc taaagaaagc gcgctggatc gccagttcga caccgcgaac ttcggccagg
     3541 cctttcagac gaccaattgc ggagtaacct gggttggttt tcttcttcag gtcgtccagc
     3601 atctgatgac cgtggtccgg acgcatcggg atcaggtctt ctttgccttc cgctttacga
     3661 cggtgttctt cttcaacaat cgctttcacc acttcgtaca tatcaacgtc accgttcagg
     3721 tgcgccgctt cgtggaaggt tttcgggtta tcttcacgca tggtggagcg cagatgggtg
     3781 aagtaaatac gcggaccgaa ctgcttgatc atatcaacca gatcgttgtc agcacgcacg
     3841 ccgtaggaac cggtgcacat ggtgaaaccg ttcgccatgc tgtttacggt atcaaccatc
     3901 cactgcatat cttcaatggt ggaaacaatg cgcggcaggc cgaggatcgg gcgcggcgga
     3961 tcgtccgggt gaaccgccat acgcacgcca acttcttcag caactggaat aatcgctttc
     4021 aggaagacgg caaagttttc gcgcagcttc gctttatcga tatctttgta cagctccagg
     4081 tgcttacgga actggtcgag ggtataccct tcttccgcgc ccggcagacc ggcaatgatg
     4141 ttacgcgtca gacgcgcttt gtcttcgtcg ctcatagtgg cgaagcgttc agctgcctga
     4201 gcaatttctt cttcggtgta atccgcttcc gcgcctggac gtttcaggat atgcatttcg
     4261 aatgcagcga attcgatctg gtcgaagcgc agagctttgg agccgtctgg cagcacgtat
     4321 tcgaggtcag tacgggtcca gtcgagcacc ggcatgaagt tgtagcacac ggtgcgaatg
     4381 ccgcactgcg ccaggttgcg cagagtctgc tgatagttag cgatccactg ctcatagttg
     4441 ccagtgtggg ttttgatatc ttcgtgaatt ggtacgcttt ctacgacaga ccacaccagg
     4501 cctgcgtctt caacgatcgc cttgcgtttg aggatctctt ctacggacca tacttcgccg
     4561 ttcgggatat ggtgcagcgc ggtaaccacg ccagttgcgc ccgcctgacg gacatcagct
     4621 aaagaaaccg gatcgtttgg gccgtaccag cgccaggtct gttccatgat ttttcctctt
     4681 tcacatagtg ggttccacag caaagagacg cggaaccctg gagttgttta ggtatccgga
     4741 tgaacaacca gaccgcagcc gtggcgttgc atcggatgag atggcgtata agttctacct
     4801 tttggtaaga catgtcaacc cgaattctga aattggttaa ccacatcaca agaatttcac
     4861 tctcatggtt gttgctgcaa attgcagcaa agggtaatcg atggttattg atcagaggat
     4921 gtgacattca tcgcaacaat ggttgaccaa tttacataac atgtcgccca aaataacaac
     4981 ggttcaacca ggtaaggtaa aacgttatgc atgtgcttaa cattctctgg gtggtattcg
     5041 gcattggtct gatgctggta ctgaatttga agttcaaaat caattcaatg gtggctttgt
     5101 tggtggcggc gctgtccgtc gggatgctgg cgggcatgga tttgatgtcg ctgctgcaca
     5161 ccatgaaagc gggcttcggc aacacgctgg gggaactggc tatcatcgtg gtgttcggtg
     5221 cggtcatcgg taaattgatg gtcgactccg gcgcggctca ccagatagcg catacgctgc
     5281 tggcgcgtct cggtctgcgc tatgtacagc tgtcggtgat tatcatcggc ctgatttttg
     5341 gtctggcgat gttctatgaa gtggccttta tcatgttagc gccgctggtt attgtcattg
     5401 ccgccgaagc taaaattccg ttcctgaaac tggcgatccc ggcagtagca gctgccacta
     5461 ccgcacattc actgttccca ccgcagccgg gtcctgtggc gctggtgaat gcttatggcg
     5521 cagatatggg gatggtttat atctatggcg tactggtgac gatcccaagt gtaatctgcg
     5581 caggtctgat cctgccgaag ttcctcggca atcttgagcg cccaacgcca tcattcctga
     5641 aagcagatca accggtagat atgaacaatc tgccctcttt cggcgtttcg attctggtgc
     5701 cgctgatccc ggcgatcatt atgatctcta ccaccatcgc caatatctgg ctggtaaaag
     5761 atacccctgc ctgggaagtg gttaacttta tcggttcctc gccgattgca atgtttattg
     5821 cgatggtggt tgcattcgta ctctttggca ccgcacgtgg tcatgacatg cagtgggtga
     5881 tgaacgcttt tgaaagtgcg gtgaaaagca ttgcaatggt gattctgatc atcggtgcgg
     5941 gtggcgtgct gaagcaaact atcatcgaca ccggcattgg cgacaccatc ggcatgttga
     6001 tgtcccacgg caatatctcg ccctacatta tggcatggct gatcactgtg ctaattcgtc
     6061 tggcgacggg tcagggtgtc gtttcggcga tgaccgccgc cgggattatc agtgctgcaa
     6121 tccttgatcc agcaaccggt cagctggttg gcgtgaatcc ggcgctgctg gtgctggcga
     6181 cggctgcggg ttccaacacc ctcacccaca ttaacgatgc atctttctgg ttgttcaaag
     6241 gttactttga cctgtcagta aaagacacgt tgaaaacctg gggtctgctg gagctggtca
     6301 actccgtggt tgggctaatt attgtgctga ttattagcat ggtagcgtaa aagaacacca
     6361 gagcctgcca atggcaggct ctgttcggat tgtcggcaaa gtgcgctttg tttatgccag
     6421 atgcgacgct gacgcgtctt atctggccta caaagggcta acgtgcaggt ttttagcttc
     6481 aggtaatatt gcgtacctgc attagcaaag ccctgtgatt tctttattga taaacaaaag
     6541 tcacgccaat aatcgattgc acattccctg cagtcacctg ccctccggta cgtgcgtaat
     6601 ttgccgttaa tcccagactt accgccgaag ttcctactgc tcctaacgat accgtgttat
     6661 tcgctggaat aatcgtaccg ttgcgcgtca actgtacgcc gacgccctgc gctggtgaaa
     6721 acgacgcggt attggtgaaa atcgagttgc ccgcatctgc ggttgtgccg gagaggtaat
     6781 accccaggtt ttggcttttc gcacagtaaa cggtaagagg aatcggcact gaaccagggt
     6841 agtccggcag agtaacggtg acatcacgag cagaaacatc gcagccgcca gtaggcacca
     6901 ccacatcatt attggcgtaa atattccaca caaactggaa atcatcgctg ttatagttgt
     6961 tggtctgtcg caaaataagc acggcaatta atgagccagc tttaatcgcc accccgcccg
     7021 cactgctcac aggcgtcaaa taaagcgcca ccggccacgg cttatccgtt ctcgaattat
     7081 aaacaacccg cggcgtttcg ctggtggtcg gaaatggata gctactgcca ctatatttta
     7141 cggtcccgga aaaattagat aacacgccgc cataagccga gcctcgttgc agtgtgacat
     7201 agtctgtaat ggtttccgga taatcgttat ggcaaaagat ttgcgtcgaa agatctacga
     7261 ccaggttttg ccccacattc acggcaggcg caaggtttac ataaacatta gcgctgccac
     7321 caccaatagg gatagcggta ccattggcgg ttttacaggc gaatgaccag gcatttaccg
     7381 accagcccat cagcagtaca gcaaacaggg taataactcg tttcattaca atcatctctt
     7441 cgggttcagc tgtaggtata ggtgatgcta atcactgcct gaatggttcc ctgagtggct
     7501 ccgccattta ccgttaatgc tctgacctgt aacgggaagt gcgctgattg tgaggaatca
     7561 tccacctgaa ctgttttggt tgcgccagta ttcaatgtgt tgccactgtc atcctgtagc
     7621 tctaactgga tgttttgcgc ggtcccctgg tttttataat atccggtact gtcggctgcc
     7681 ccgctgaagc tggcagtgac cctcgacgtt cccaccggac aattagtcaa ctcaagcgca
     7741 acatcatgcc aggccgatgc cgccccggca gacatcagac tgaaagaata aagatcgccg
     7801 agatcaaccg tggcattggt ggtggaaact gtgcacggtt tggcgacgac cttaccgttc
     7861 accgtgatgg tgacatcggc tgcctgtatc gtcgcacttg cgagcgccaa tattgccgcc
     7921 aataaatacc cacgtttgcg ccatttcatg agcatctcca gttactgata ttcaagagtg
     7981 aaggtagccg tggcattgat atgccccgca gtgacaggca cctgtgtcgc cattagccgg
     8041 gcgtaaaaat tcagcatatt tggtttaccc ggcgtcaggg tcgtccacga aagcgcggac
     8101 gatggagcat taaggggtat ctgattttgc tgctcattca gaagctgtat tcccagtccc
     8161 gaagccgctg acaccgtatt ttcaagtgca agcaggttgg cattgtggct atctgcaaca
     8221 ccggtaaacc caacttttac ggcagaaacg gcattaccac agggtgacag caaaatacga
     8281 aatggaacga caggagtcgt cgcgccaatg ttgttaaatt gcttcgccgc gttttccatc
     8341 aaatcaacag taaaattggt tgattcagcg gccacactac agccgttatc tctgacatag
     8401 ccgcggatag taatcgtgct atccgcagcc aaaacgcgac ttaccgccag ccacaaaaaa
     8461 gcgcacagaa gataaaaagg tttgtttctc atcacgcccc cttaacgaca ttcagctgat
     8521 agctgggtta ataactgctg ctgactctct ggtggcagtt gataattggc gacacaatga
     8581 gcattttcct cttctcccca tttcacctga acttttcccg ctagaggcat tccgctgagg
     8641 taaacctgac cattatccgc aacaatgccg ctactctggc tactctctga tgtcaccatc
     8701 gccccaaacg gcagcggctt attattgtgg gttagcgtca tgagcagttt tatcccaacg
     8761 cgcgctttaa actctgctcg cacgatcgcc ccacgagtgg gaacaacgtt agcgaccgcg
     8821 ttatctaaat cgacgttatc agccagggta ttggtatcca gcgccactct gttttcccga
     8881 tattcagtgg cataaggcag cacggcataa ccacgccagt cggtacgcac ccccgtctgg
     8941 ttttcgactt ttgcatcttt tgcgccaggc gctttaacaa gcaccaccgt atcgtttaac
     9001 ggctgcccca gcgttacgcc attggcatga gccagtaccc caccgctgac tccgtaatag
     9061 agctgcttaa tatcatcgct atggctgtaa ccgatattgg cattgccgta accaccgcga
     9121 taattcagcg tggcgtagcc tgtacttccg ctattaccat cgcctccccc ggcatagccg
     9181 gtttgcacgc tataactgag gttgttgtct tccagcaacg taccgtatac accagccaga
     9241 ttggtcatcc gaccgttgag atcgtgtgac atgctgtagc tagcactggc atgtcgccac
     9301 tgagatttac tgtcagaacg cagccagtgg ctgaaaggaa tattgacgtt aagcgctaac
     9361 atctgatccc gtcctttttg ccaggcgttt ttcgtcaggc tatagctgag cgtccagttg
     9421 atatcttcga acgcagtatt taatccagcc tggaattgct catcgacatt actcgttccc
     9481 caataagttt gatggctacc actcaaatac agtgttgatg tgcgcccgag ttgctgagta
     9541 acggtgagct gtaatttccc gcgtttgtta taagcgaggt tgtaatagtc ggtgaatttc
     9601 ggcttaacct gaataactcc atcctgtgtt tcgatgttgt agccattcat tcgactgtat
     9661 gttgtatcag cgaaattaaa atatccgctg gtcgaataac ggtaacccac taactgaata
     9721 ttcgtgcctg actcattgag cgatttgtta tagagaaaac gcaccgattg tccgtcatgc
     9781 tgactgtcat cgggaagtgt ggaattagcc tgcgtcatat ccacagacag agcgcccagt
     9841 gcccccatgt ttttcccgat accgaaatta aaagcacgat aacgatccgc caattgcgtt
     9901 ccaccatata ttgtccagcc agccggaagg ccgtggagta atgtactctg gaaaaagcgg
     9961 ggtttttcct gctgcgcatt tccactacgg tattctcctg ccgtaatgga ataacgagta
    10021 tgcccttcac gttgcaaaag cgggactgac gaatagggta cggtaaaaat ctgcgtgctg
    10081 ccgtcagcct ctttgatcgt tacctgcaag tcaccactat tacctgcggc atagatatcg
    10141 ttgatggtaa aaggccccgg tggcaccgta ctattataaa tgtcataccc attttgttta
    10201 atagtgacct gtgcagtacc acgagcaata ccgtggatca ccggggcaaa tcctctttgg
    10261 ctatcgggta acatattgtc atctgaggcc aattgtgcgc cgcgaaagtt aataccatcg
    10321 aaaatatcgc cctgggtata accatcaccc agcgtcagcc gggaacgtaa cggtattatg
    10381 tctcgctcaa gccaggtatt gatatgctgc catttatttt tgctacctga tgaactgtcg
    10441 ctactgttat aactccagct ggtattgtcg cgtaaacgcc acgcaccaat atttaaccca
    10501 ctctgtaggt ttaaatatgc ataatggctg ttacccccaa tccgattctg tacactattt
    10561 ccgctgaaat tataattgag caatccggca ttaataccgg gatcccataa ctcaggagga
    10621 atataaccac gcgcgcgatt actcataaat gcctgaggga tcgtcaggtt gagtcgctgc
    10681 tgaccaacat ctaaatgcgc agtagcgtcc tggaccattg tggttaatgg cacacaggca
    10741 tcatccgcca gcagattcat accggagaca gaagccgtat tcagccccat actggcaagt
    10801 tgcgcgcgtg tcaggcaggg aacaatccct tgttcgctgt cgcccgtatt aaatgtgaca
    10861 tcacgcgttg ccatataacc attattcaaa tagatatcga cacgatacgt ccctggcggt
    10921 aattcttgcc cattttcaaa acgcgataaa tcggccacag cctggggatc atccgctaaa
    10981 aagcgcggat taaaatagag ttcggcagat gacaaaggtg cctgtgcggc aaaagcacag
    11041 gcgacaacga gccggacaaa aaaaccagcc aaacgatgct tacgaatatg caagcattgt
    11101 gtgtttcgct ggtaaagtct taaattcaga tatgacatta ttcctccggt ttaaaatcac
    11161 ccggcagtca attcttttta ttcaggcgaa aaattccccc tgcgttattc cattacgccc
    11221 gtcattttgg gggtaagtgc gccataatca tttattgttc ggtaagtaat attgcttcct
    11281 gcatcagaag gcaatttaac cgtgctttcg cccattggag gcaccaatgc attttcaaga
    11341 acccgggttc cggcattcaa ctctgttacc gtcaggtaat agggtgtcgg gttaatcagc
    11401 gtcagagaat tcgcgctacg acgaaatctt aatttttctg cggcctgatc gggtggcaac
    11461 gctaatttag ccggacgata gtacagttta atgcggctga taattgcgag ctgtagcgta
    11521 ttctcagtca attttgattt atccattgac ggaatcgctt taacgttcat ccagaataaa
    11581 ctttcccggt cctgtggcaa ttggttattt gttgcatcaa gaatacgcaa ggtattctct
    11641 ttttttccct tcatcgcaaa cagaggaggc gtcacgataa aacgaccatc ctttacacca
    11701 tcggcatttt ccacccatga ttgaattaaa taggtactat tttcatcatt atttgtcacg
    11761 gcaagttgca cttgtttttg ccctgccgga taaattacgc gagtcgcacc taaggccact
    11821 cccgcttcag cgcgtccggc aaccatcatt gccatgaaca tcaggatacc tgccagcaag
    11881 cagaatgtta tttcctgcga tttccttaca ttgacgtttt tattactcac tgtcctgttc
    11941 ctgttatcac attatctgct gaacaattac tgataggtta aagagaacca ggcctgggca
    12001 ttggcgatgc cgccagtaac ccgacgcccg gtagcacgat atttggcgat gaaatgtagc
    12061 gaagtagagc ctgagtaaag ccgtttccag tttgctggag gacgattaat cggtacgagg
    12121 tttccttcat catcaaacaa cgctacgcca atattggtgg ctatccctgg cccctctccc
    12181 acggaaagca catccggatt tttaccattc gcgacaccgt gaaacgccac acccacacgt
    12241 tcactcacca ccgtgctaca ttcccgtaaa tgaataacaa aaggcaccgg tgcgctatct
    12301 tcccctaccg catgaaaccg gttactgctg atttgcccca tattgaccgt catttgttta
    12361 tcaccggctt caatccggca agtttccgca ataatgacgc cctgaaattg catatttccg
    12421 ccgggcaacg tggtattcca tttatttccg gccagagcaa acattggcag caacgacgct
    12481 aatagaaata atctcttcct tttcatcgtt atttttatcg cacaagggtg ggcatccctg
    12541 cccgtaatga cgtccctgaa cctgggtagg ttattgatac tgaaccttga aggtcgcatc
    12601 cgcattagct gcgccagggg ttgcggcacc aattgcataa taacgcgcct ggaacggaat
    12661 ggtgttggtt ccgttattca gggttgtttg tgcactaaat gacgcaccat ccagcgccaa
    12721 cgcagctcct gttttatcca ggatctgcac accaacgttt gttgcgctac ccgcagcaga
    12781 actctgcaga gccagtacgt tggtatgatt cgtgtcaatt gttgtaccta agaaggcaac
    12841 agcagctttg cttgcaacat cggtatcgca atcattcagc tgaatgttaa aaccgacagc
    12901 agagctggtc gctccatcct ggtccagcga agcggtacga acctgaccta actgaacggt
    12961 ttgatcaaca gagcctgcat caactgcgca agcggcgtta acaacttccc ctttaaagtg
    13021 aacggtccca ccattaaccg tcgtggcagc ggccagagcc gctgtagaac tgagggacag
    13081 agccgacaga acaacgattg ccagagtttt aattttcatg ctgctttcct ttaaaaaaac
    13141 tatttctaaa tcgacatggg cagtcgttct gtacactttg ttttgtcaac gagtttgact
    13201 gccaacactg cacagttttc ccccaaaaga tgaaacattt ggggccattt tgactcatag
    13261 aggaaagcat cgcgcacaaa ctttttcagt ttatttgttg gcttaatatt cta
//
LOCUS       DAZUCE010000132        13262 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZUCE010000132 DAZUCE010000000
VERSION     DAZUCE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.132"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..248
                     /locus_tag="ACHY03_003269"
     CDS             <1..248
                     /locus_tag="ACHY03_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906463.1"
                     /translation="FDLDTPCIFNAPAYNKSNEKSLERVSENGLSGVLSDRNKKIKLI
                     TGVAPFDDILFMDDDFDDNSPEDGPVENSPVVNSPLV"
     gene            618..776
                     /locus_tag="ACHY03_003270"
                     /pseudo
     CDS             618..776
                     /locus_tag="ACHY03_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1000..1119
                     /locus_tag="ACHY03_003271"
     CDS             1000..1119
                     /locus_tag="ACHY03_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFS3906464.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            1184..5764
                     /gene="ypjA"
                     /locus_tag="ACHY03_003272"
     CDS             1184..5764
                     /gene="ypjA"
                     /locus_tag="ACHY03_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HFS3906465.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTTNVIGVVNDETV
                     DGVQKVDERGTTNNTHIINHGRQEVYGGISNSSIIETGGEQLVSIHADINGQANNTTI
                     NGGRQSIEYGGISTGTIIESGNQYVHKGGTSNDTTIKGGTSRIEGGTANGTIIDGGSQ
                     RVTTQGHVDSTTINKSGSQDITQGSLATNTTINGGRQYVEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSSSDVIEVYSGGVLDVRGGT
                     ATNITQHDGAALKVTTYDLTVSGTNSEGAFSIHNKVADNVLLENGGHLEVSHSANKTI
                     IKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNINSG
                     TQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETGSA
                     LVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGKLI
                     VQKEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTFIEGTNSYGDAFY
                     IRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVNGKDVGTVLNSAGTQTIYAS
                     ATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAINTD
                     IVSGLQQIMANGTAEGSIINGGSQIVNEGGLAENSVLNDGGTLDVREKGSATGIQQSS
                     QGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTTSAKTQVNTGG
                     QEIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTTINEGGTLTV
                     NDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSISGNLATNALLENGGNLLVLA
                     GTEARDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQPLARAEDLQVAGGTAI
                     VYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLADLT
                     AASRGSVWLNSNNSCAGTSNCEYRVNSLLLNDGNVYLSAQTAAPATTNGIYNTLTTNE
                     LSGSGNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGGDA
                     SFTLGNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDPKP
                     DPKPDPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHN
                     NNVWGATYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIG
                     FDRGGHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSN
                     GLGGHIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMKSKSVDTRSIYRELGAT
                     LSYNMRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASF
                     SSTLSGHLGVGYSNGADVESPWNAVAGVNWSF"
     gene            complement(6128..6457)
                     /locus_tag="ACHY03_003273"
     CDS             complement(6128..6457)
                     /locus_tag="ACHY03_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFS3906466.1"
                     /translation="MQTLSSHPTRATQPCLSPVETWQRLLTHLLSQHYGLTLNDTPFS
                     NETTIREDIDAGVSLSDAVNFLVEKYELVRIDRKGFSWQEQTPYISVVDILRARRSTG
                     LLKTNVK"
     gene            complement(6495..6860)
                     /locus_tag="ACHY03_003274"
     CDS             complement(6495..6860)
                     /locus_tag="ACHY03_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFS3906467.1"
                     /translation="MNNHSVFGTKPENLTCQEWGLKRTITPCFGARLVQEGNRVHFLA
                     DRAGFNGAFIDDNALRLDLAFPLILKQLELMLTSGELSPRHEHCVTLYHNGLTCEADT
                     LGSCGYVYIAIYPDQPEPH"
     gene            complement(6886..7107)
                     /locus_tag="ACHY03_003275"
     CDS             complement(6886..7107)
                     /locus_tag="ACHY03_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS3906468.1"
                     /translation="MKIISKRQAMALYRQHPGSRLFRFCTGKYKWTGSICHYAGREVQ
                     DIRNVLAVFAERRQDRNGPYVILRSVTLN"
     gene            complement(7104..7646)
                     /gene="radC"
                     /locus_tag="ACHY03_003276"
     CDS             complement(7104..7646)
                     /gene="radC"
                     /locus_tag="ACHY03_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS3906469.1"
                     /translation="MNTVLCPESSQRELFPLTVMAQHGYLLPATSGLTPYAQRTIRRA
                     INLLDKYLRQPGISFTSSIAARDWLRLQLAGQEREVFMVLYLDNQHRLLESETLFAGS
                     VYHVQVHPREVVKSALRFNAAAVVLAHNHPSGDPEPSKVDRQMTDKLKEVLGLVDVKT
                     LDHLVVGQDGIVSFAERGWI"
     gene            complement(7659..8102)
                     /locus_tag="ACHY03_003277"
     CDS             complement(7659..8102)
                     /locus_tag="ACHY03_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS3906470.1"
                     /translation="MDTQNLQFAEQTAITASVVPDELRIGFWPQHFGSIPQWITLEPR
                     IFAWMDRLCTDYHGGIWHFSTLSNGGAFMAPESEQDEKWKLFNSMNGNGAELTGEAAG
                     IVACLMVYSHHACRIECDAMTEHYYRLRDFALNHPECSAIMYLID"
     gene            complement(8133..8954)
                     /locus_tag="ACHY03_003278"
     CDS             complement(8133..8954)
                     /locus_tag="ACHY03_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS3906471.1"
                     /translation="MRLASRFGRINQIRRDRPLTHEELMSHVPSVFGSDKHESRSDRY
                     TYIPTITILESLQREGFQPFFACQTKVRDQSKREHTKHMLRLRRAGQLNGHQVPEIIL
                     LNSHDGSSSYQMLPGLFRGVCTNGLVCGQSFGEVRVPHKGNVVEKVIEGAYEVLGVFD
                     RVDEKRDAMASLLLPPPAQHALANAALKYRFGEDHQPVTVSQLLTSRRREDCSDDLWT
                     VYQRVQENLMKGGLSGRTAQGKSSRTRAVTGINGDVKLNRALWVMAENMLEFFGR"
     gene            complement(9046..9387)
                     /locus_tag="ACHY03_003279"
     CDS             complement(9046..9387)
                     /locus_tag="ACHY03_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906472.1"
                     /translation="MKKIITVAALSLLSFSSLAGSSPVSVSVSPGSYSHYSSIRVTSK
                     VDSIVIKQLIVNRGNCQDAEFASPWKSVRLGFGGAVSHEFTGKGLMVPCNVLEVVVQT
                     SEGAWQFDFDS"
     gene            complement(9422..9886)
                     /locus_tag="ACHY03_003280"
     CDS             complement(9422..9886)
                     /locus_tag="ACHY03_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906473.1"
                     /translation="MKNKLVISFLLASVLTPSVSHAFGNPDSWVSGYAQGTSEYTILG
                     KGQSQLYLACDSSGSQPATIIFTDVNGQQVRMDSGQTLAMRIDNAEEVNISESESHVG
                     SDNVMWAWNKLRTGKRVVVSGSGVKPATFTLAGAAAVLPAFGDNGCVPGFAL"
     gene            complement(10012..10170)
                     /locus_tag="ACHY03_003281"
     CDS             complement(10012..10170)
                     /locus_tag="ACHY03_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906474.1"
                     /translation="MPLILLWGGLALLLGIVASANGRSFWGWFILGLIIDPILAGLLY
                     WLIAKDRT"
     gene            complement(10392..11279)
                     /locus_tag="ACHY03_003282"
     CDS             complement(10392..11279)
                     /locus_tag="ACHY03_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFS3906475.1"
                     /translation="MKHPSGYTLIRRHLRRYPHALRQHLLQELNRLVTYEPVIGIMGK
                     TGVGKSSLCNALFRSEVCTVNAVEACTRQPQRVRLRFGSHYLTLVDLPGVGESVTRDG
                     EYRELYRDLMPQLDMVLWVLKADDRAFAVEEQFYQDVFAQFRGPIPPVLWILNQVDKT
                     EPAEQWRWLSAQPSALQAERIAQKRQAVARQLQIAEIDILPVSVRGRYRLPRLVETMI
                     TRLPKQARSPLVPHLQNRYRTDTVINTASSSFGDAVVEVIDNIIDLAPLPQVARTALH
                     AVTHTVARAAGSLWGFFFS"
     gene            11594..13051
                     /locus_tag="ACHY03_003283"
     CDS             11594..13051
                     /locus_tag="ACHY03_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFS3906476.1"
                     /translation="MQEFPFNAFPSSLLETIFDIEECTRAPGEMIALTLLSGLSLACQ
                     SLINVKITDTMKSPVSLYSFVIADSGERKTAVEKLLLKPFHEHDLRVLLQYEKQKKEH
                     EVQSKIWSKKENALLKCIDKDTMKGLCTDKLSRQLEQLSAEKPVQPKAKRYIYNNVTP
                     EALQFEMYTHSPQTGLITDEGANILSRRTMSDLGFMNSIWDDESFHVNRKTGPSFTIK
                     NGRITLLVMVQKAIFYDFLKRKGEHARGSGLFARCFILYIDAKLSTQGQRFISRLSSP
                     QSGKLENLNRFYQRMNELIEKSASQHGTGNQTCLSFEPSALAAWKEIHDEIESSIGPD
                     REYANMNDFASKLPNNVARLAALLSYYTEGECAIKKEYVENAWLLCEWYMQQAIKVFG
                     AQEGYYEALLLSWLRREYYETEMDHVRFNTIRNAGPNVLRKGKLLERVIERLEKEGAI
                     DIVRSRRKARMVYAGRYFRNNPFTKNAAYKHYWPR"
BASE COUNT         3633 a         3007 c         3393 g         3229 t
ORIGIN      
        1 tgtttgatct tgatactcca tgtattttta atgcgcctgc ttacaacaag agtaatgaaa
       61 aatcattaga acgcgtcagc gaaaacggtt taagtggagt cttgtctgat cgtaataaaa
      121 aaataaaact aatcacgggt gtggcaccat tcgatgatat tttatttatg gatgatgact
      181 ttgatgataa ctcccctgag gatggtcccg ttgagaatag tcctgttgtg aatagtcccc
      241 ttgtatgaag ttctaaaagt agttttcaat gataaataaa tggctatttt tacgaagaga
      301 taataacgta gattgaaaca atatgtatta ttgattgagt aaacgggatt attcttcaca
      361 ctacatccat tttagtgtca ctaccaaaga aaaaggggct acgttttcat gtaacccctt
      421 gatttatttg gtgggctggc agtgactgaa ttggtgctgt aattggttga aatatttcat
      481 ctaatgtctg gttcaacttt ccttagctgc ctgaaagttt tttgatctta cccagcaata
      541 acagcgttac ggtgcaccac gcctgactga agaactgcgt gctcagggtt accacttcaa
      601 cgtaaaaccg tggcaggagg tccggactga aaaaactgct caggacatca tcggcaggga
      661 acgcactgct agtctgtaaa ctgaaccgac tggggcgcag tatgtggcat cttgtcgtgc
      721 tgttggagga gctgtgcgaa cgtggtatta actttcgtgc tctggcccaa tctatatttg
      781 cccaacaatg gggggacgaa tgctgtaaaa gtaaaacaat ctgcgatctc aaagttattg
      841 tgtgattttt atgtgagcag aagatattca tcagaaacga ttatgtaaat cattttattt
      901 tgccaacggc ctcattgtcg aaagaaaaga atacgcccgt gtttcctgaa tagattgact
      961 tttttatcca accacacttc agcgcactgc gtttaaaaaa tgcctcattc ttatgcggaa
     1021 tatcatcatt tcatcatgat gtctttgatg agcggtgaac acaatacact tgcgctgtct
     1081 cttcaggatg aatcccctgg tctggtgcct gtgggctgat gttgcagcaa agctaaggtc
     1141 gcttaaacgc tactcagtat tcacttttca gaggatgaaa tttatgaaca ggaccagtcc
     1201 ctattattgt cgccgctcag tactttcctt attgatatct gccttgatat atgccccgcc
     1261 cgggatggct gccttcacta ctaatgttat tggtgtggta aacgatgaga ctgtagatgg
     1321 tgtccaaaaa gtagatgaac gaggtacaac aaataacact catattatca accatggccg
     1381 gcaggaggtc tatggtggga tatctaatag tagtattatt gaaactggtg gcgagcaact
     1441 agtatcaatc catgccgata ttaacgggca agctaataat acaaccatta atggaggcag
     1501 acagtcaatt gagtacggag gaatttctac tggcacgata atcgaaagtg gcaatcaata
     1561 tgttcataaa ggtggtacca gcaatgacac gacaattaag ggcggtactt cacgcataga
     1621 gggggggact gcgaatggaa caatcattga tggtggcagc caacgagtaa caactcaggg
     1681 gcatgtcgac agtacaacga taaataagtc tggctctcag gacatcacgc aaggaagtct
     1741 ggcaacgaac acaaccataa atggtggtcg acagtatgtt gaacagagca cagtagaaac
     1801 aaccaccatt aaaaatggcg gtgagcaaag agtatatgag agccgtgcgc tggacacgac
     1861 gattgaaggc ggaactcagt ctctgaatag taagtcaacg gcaaaaaata cgcagatcta
     1921 ttctggtggt acgcaaattg ttgataacac gagctcctcg gatgttattg aagtttattc
     1981 cggcggcgtg cttgatgtta gaggtggtac ggcaactaat attacgcagc acgatggagc
     2041 cgctttaaaa gtaacgactt acgatttgac ggtgagcggt acgaatagtg aaggggcatt
     2101 ctccatccac aataaagtgg ctgacaatgt gttgctggaa aacggaggcc atttagaagt
     2161 atctcattct gcaaacaaga cgattatcaa agataaagga acaatgtcag ttttaaccaa
     2221 tgctaaagct gatgcgaccc gaatagataa tggcggggtt atggatgttg ccggaaacgc
     2281 gacaaatacc ataattaatg gtggcacaca gaatattaat aattatggca tagccacagg
     2341 caccaatatc aacagcggaa cgcaaaatat caaaagcggc gggaaagctg acacaacaat
     2401 tatatcctcc gggagccggc aggttgttga gaaagatggt acggcaattg gcagcaatat
     2461 tagcgccgga ggctcgctga ttgtctatac cggcggtatt gcacatgggg ttaaccagga
     2521 gacgggcagt gctttagttg ccaacacggg tgcagggact gatatcgaag gatacaacaa
     2581 gctctctcac ttcactatta ccggagggga ggctaattat gttgtgctgg aaaataccgg
     2641 cgaactgacg gtagtggcta aaacctcggc gaaaaatact accattgatg ctggcggtaa
     2701 gctgattgtc cagaaggagg ctaaaacaga tagcaccaga cttaataatg gcggcgttct
     2761 ggaggttcag gatggtggtg aggctaagca tgttgagcaa caatccggcg gcgcattaat
     2821 tgcttccacg acatccggaa catttatcga aggaaccaac agttatggtg atgctttcta
     2881 cattaggaat tcagaagcta aaaatgtagt gctggaaaac gctggctcat taacagtcgt
     2941 cactggttcc cgggcagttg acacgattat taatgccaac ggcaaaatgg atgttaatgg
     3001 aaaagatgtt ggcactgtac tcaatagtgc tggcacccaa acaatatatg ccagtgccac
     3061 ttctgataaa gcaaatatca aaggtggcaa gcaaacggta tatggtttag ccactgaagc
     3121 aaatatcgaa agtggtgaac aaattgttga tggtgggtca acagagaaaa cacacatcaa
     3181 tggtggcacg caaaccgttc agaattatgg taaggcgatc aataccgata tcgtctctgg
     3241 cctacaacaa attatggcaa acgggacagc ggaaggttcc attattaatg gcggttcaca
     3301 gatagttaat gagggcggtc tggctgaaaa ctcggtgctt aatgacggcg gcacactcga
     3361 tgtgcgggag aaaggcagcg caacggggat acagcaaagt agccagggcg cgttggttgc
     3421 aaccaccagg gcgacgcggg tcacagggac acgcgctgat ggtgtcgcgt tcagcatcga
     3481 gcagggtgcg gcgaacaata tcctgctggc aaatggcggc gtgttaaccg tggagtcaga
     3541 caccacttcc gccaaaacac aggtcaatac gggcgggcag gagatcgtca aaacaaaagc
     3601 cactgcgaca ggcacgacgc tcaccggcgg cgaacaaatc gtcgagggtg tggcgaatga
     3661 gacaacgatt aacgacggtg gaatacaaac agtttcggct aacggagagg caataaaaac
     3721 aacgatcaat gaaggcggta cgctgacagt taacgataat ggcaaagcaa cagatatcgt
     3781 ccagaacagc ggtgccgctc tccagacgag cacggctaac ggtattgaaa tcagcggtac
     3841 tcaccagtac ggcacttttt ccatttccgg caatttagcg accaatgcgt tgctggaaaa
     3901 tggcggtaat ttattggtat tagcaggcac cgaagctcgc gactccacgg ttggcaaggg
     3961 tggtgcaatg caaaacctgg gtcaggactc cgccacaaag gttaactctg gcgggcaata
     4021 tacccttggg cggtcaaaag atgagtttca gcctctggcc cgggcagaag atctccaggt
     4081 cgctggcggg acggcaatcg tctacgcagg tacgctggcg gatgcatcgg tcagtggcgc
     4141 gacaggaagc ctgtcgttaa tgacgccacg ggataatgtt acgccagtta aactcgaagg
     4201 ggcggtccgg attaccgata gcgcgacatt gactctggga aatggcgtcg atacaacgct
     4261 tgccgacctg acggctgcca gccggggcag tgtctggctt aacagcaata attcctgtgc
     4321 aggcaccagc aactgcgagt atagagtaaa cagtttgcta cttaacgacg gtaatgttta
     4381 tttatcagca caaacagcag cgcctgccac aactaacggt atatacaata cgctgacaac
     4441 caatgaactt tccggtagcg gtaatttcta cctgcatacc aacgttgcag gctcccgggg
     4501 cgatcaactg gtcgtcaaca acaacgccac tggtaatttt aaaatctttg ttcaggatac
     4561 cggcgtcagc ccacagtctg acgacgcgat gacgctggtg aaaacagggg gaggggatgc
     4621 ttcgtttacg ctgggcaata ccggcggttt cgttgatctt gggacctatg agtatgtcct
     4681 gaaaagcgat ggcaacagca actggaacct gaccaatgat gtcaaaccca acccggatcc
     4741 caacccaaat cccaacccaa atccgaagcc agatccaaaa ccagacccaa aaccggatcc
     4801 gaaaccagac ccgactcccg atccaacgcc gacacccgtt ccggagaaac gcatcacgcc
     4861 ttctacggca gccgtactca atatggctgc aacattaccg ttggtatttg atgctgagct
     4921 aaacagtatt cgcgagcggt tgaacataat gaaagcgagt ccacacaaca ataatgtctg
     4981 gggggcgacg tataacaccc gtaataatgt caccaccgat gcgggggccg ggtttgagca
     5041 gacgctgacc ggaatgacag tggggatcga cagccgtaat gatattcctg aggggattgc
     5101 gacgctgggc gcttttatgg gttattccca ttcacatatc ggttttgatc gtggaggaca
     5161 tggcagtgtg ggcagttatt ctctgggcgg ctatgccagt tgggaacatg aaagtggttt
     5221 ctatctggac ggtgtcgtga agctgaaccg ttttgaaagt aacgtagccg gtaaaatgag
     5281 cagcggtgga gccgccaatg gcagttacca cagcaacggg ctgggcggtc acattgaaac
     5341 cgggatgcga tttaccgatg gtaactggaa cctgacgccg tatgcatcgt taacggggtt
     5401 caccgctgat aaccccgaat atcatttatc caatggcatg aaatcgaaat cagtcgatac
     5461 ccgcagtata tatcgtgaac tgggcgcaac gctgagttac aacatgcgtc tgggaaacgg
     5521 tatggaaatt gagccgtggc tgaaggcggc tgtgcgcaaa gaatttgtcg atgataaccg
     5581 ggtgaaggtg aataatgacg gtaatttcgt caatgatttg tcgggcagac gtggaatata
     5641 ccaggcaggt attaaagcct cattcagcag tacgctaagc gggcatctcg gggtggggta
     5701 tagcaacggt gccgatgtgg aatccccgtg gaacgcggtg gctggtgtga actggtcgtt
     5761 ctgaccatca acgaaaaagc ccacatctgt gggctttcat gtcaccagga gccgcggctc
     5821 ctttgcgtat ccttttatgt ctcctcaccg tctggtcggt gtcctgctaa gacttctaac
     5881 ttcctgtttt tgttggtgtt gtccttacac cgtccaatca tgattggtgg gttggcggag
     5941 tctgaattgg gtttgtaact tgttgtttaa tggctgataa ccttcagttc aattttcatt
     6001 ttggtcccat tgttggtcct aaaagaaaat gtcaggtatt ttgcagggag gggaggttac
     6061 agcaggagtc tcttcgtctg tgagattagc attttgcttt ccttaagtct gctctgtatt
     6121 taagcgttta tttcacatta gtttttagca agccggtaga gcgccttgct cgcagaatat
     6181 ccactacgga aatatacggg gtttgttctt gccacgaaaa tccctttcgg tcgattcgaa
     6241 ccagttcgta tttttcgacc agaaagttca ccgcatcgct gagcgatact ccggcatcta
     6301 tatcttcacg tatggtggtt tcattactga acggtgtgtc attcagcgtg aggccatagt
     6361 gctgcgataa cagatgagtc agcagccgtt gccaggtttc aacgggtgac agacagggct
     6421 gagtcgccct tgtcgggtgt gatgataagg tttgcatgat gtttgctcta tgagtttaag
     6481 agataagcgt agagttaatg gggttcaggc tggtcgggat aaatagcgat gtagacatag
     6541 ccacaggagc cgagtgtatc ggcttcgcaa gtcagtccat tgtgatatag cgtgacgcag
     6601 tgctcatgcc ggggagagag ttctccgctg gtgagcatca gctccagttg cttgagtatc
     6661 aacggaaagg ccagatccag gcgtagcgcg ttatcgtcga tgaaagcacc gttgaatccg
     6721 gccctgtcag cgagaaaatg cacccggtta ccttcctgca ccagacgagc gccaaaacag
     6781 ggggtgatcg tgcgctttag tccccactcc tggcaggtaa gattctccgg tttggtaccg
     6841 aatactgagt gattgttcat tattgttcct gattaaaggg aaggattaat taagagtgac
     6901 acttcgaaga atgacatagg gaccattacg atcctgacgg cgctcagcaa agacggccag
     6961 aacgttgcgt atgtcctgaa cttcccggcc agcataatgg cagatactgc cagtccattt
     7021 atatttgccg gtacagaagc gaaacagtcg ggagcccgga tgttgccggt acagagccat
     7081 ggcctggcgc ttactgatga ttttcatatc cagcctcgtt ctgcaaacga cacaatgcca
     7141 tcctgaccga caaccaggtg atcgagcgtt ttcacatcaa ccagtcccag cacctccttc
     7201 agtttgtcag tcatctgccg atcgactttg ctgggctccg gatcgccgga tggatggttg
     7261 tgcgccaata cgacagcggc agcattgaag cgaagtgcag actttaccac ttcacgggga
     7321 tggacctgga catggtaaac cgaaccggca aacagcgttt cgctctccag taaacggtgc
     7381 tgattatcga gatagagcac cataaacact tcccgttctt gtcctgccag ctgtaaccga
     7441 agccagtcac gggcagcaat acttgatgta aaggatatgc cgggctggcg cagatattta
     7501 tccagcagat tgatagcccg gcgaatggtc cgttgggcat agggtgtcaa tccggaagtc
     7561 gcagggagca ggtaaccatg ttgagccata accgtcagcg gaaatagttc gcgctgtgaa
     7621 gactccgggc ataatacggt attcatgtca ggtatccctc agtcgattag atacataatg
     7681 gcactgcact ctggatggtt tagcgcgaag tctcgtaagc gataatagtg ctctgtcatc
     7741 gcgtcacatt cgatacggca ggcatgatgg ctgtatacca tcagacaggc gactatccct
     7801 gcagcttcac cagtaagttc cgcgccgttg ccattcatac tgttgaacaa tttccacttc
     7861 tcatcctgct ctgactcagg ggccataaaa gcgccgccgt tgctgagcgt cgaaaagtgc
     7921 cagataccac cgtgataatc ggtacacaga cggtccatcc aggcaaaaat tctgggctca
     7981 agggttatcc attgcgggat ggagccaaag tgctgtggcc agaagccgat gcgtaactcg
     8041 tcaggtacca cagaggcagt aattgctgtc tgctcagcga actgaaggtt ttgtgtatcc
     8101 atgaaactat ctccttatgc ttgtatcgtt gtttaacgcc cgaaaaactc gagcatattc
     8161 tctgccatta cccacagggc acgattgagc ttcacgtcgc cattaatacc ggtaacggca
     8221 cgggtgcgac tgcttttccc ctgtgcggtt cgtcctgaca atcccccttt catcaggttc
     8281 tcctgcacgc gctggtagac ggtccacagg tcatcgctgc aatcctcccg gcgacgtgag
     8341 gtcagcaact gcgataccgt gaccggctgg tggtcctcac caaagcgata tttcagcgcg
     8401 gcattcgcca gagcgtgctg tgctggtggc ggaagtaaaa gtgacgccat ggcatcacgc
     8461 ttttcatcca cccggtcaaa taccccgaga acttcgtaag ccccttcaat taccttctca
     8521 acgacattac ctttatgtgg tacccgcacc tctccgaatg actgaccgca gaccagaccg
     8581 ttagtacaga cgccacgaaa caaccccggc agcatctggt agcttgatga gccgtcatgg
     8641 ctgttgagta aaatgatttc cggtacctga tgaccgttta gctgcccggc gcgacgcagg
     8701 cgaagcatgt gtttggtgtg ctcccgtttg ctctggtccc gaacctttgt ctggcaggca
     8761 aagaacggct gaaaaccctc gcgctgcagg ctttcgagga tggtgatggt cggaatgtag
     8821 gtgtaacggt ctgaacgcga ttcatgtttg tcactcccga atacactggg aacatgactc
     8881 atcagctctt catgggtcag aggacggtca cggcgtatct ggtttatacg gccaaagcgg
     8941 ctggctaatc tcataagtaa ttcctttaag agaggcaaaa gaaaacgccc tgctcgtttg
     9001 aggagggcgt tcagatagaa aagaaaggtg gaaaagcgcg cggtgtcagc tgtcaaaatc
     9061 aaactgccat gcgccttcgc ttgtctggac aactacttcc agtacattac agggaaccat
     9121 cagccccttg ccggtaaatt catgggatac agcaccgcca aatcccagac gtaccgattt
     9181 ccaggggctg gcgaattctg catcctggca gtttcccctg ttgacaatga gttgtttaat
     9241 cacaatcgaa tcgacttttg atgtaacccg aatcgatgaa tagtgactgt agcttcccgg
     9301 gctgacgctg acgctaacag gtgaagaccc ggccagcgaa gaaaatgaca gcagactgag
     9361 tgctgcgact gtaataattt tcttcattaa atttccttta atctgtaaga gtgaggcaga
     9421 gctacagtgc aaatccgggc acacagccgt tgtcaccgaa ggcaggaaga actgcagcag
     9481 cccccgctaa agtgaaagtg gcaggtttta ccccgctacc tgaaaccaca acacgtttgc
     9541 cggtgcgtaa tttgttccat gcccacatca cattgtcaga tcccacatga gactcgcttt
     9601 cgctgatatt cacttcttca gcgttatcaa ttctcatggc aagtgtctgg ccgctgtcca
     9661 tcctgacctg ttggccgttc acgtcagtga agataatggt cgctggctgt gagccggaac
     9721 tatcgcaggc cagataaagt tgtgattgcc ctttaccgag gatggtgtat tcagacgttc
     9781 cctgcgcata gccacttacc cagctatcag ggttgccaaa cgcatgcgag acagaaggcg
     9841 tcagcactga agccagtaaa aatgaaataa ccagtttatt tttcattgga gtgtcctgtc
     9901 agaagccagc catattgccc agggtggtga taatgagcag taggcaggcc ataaagataa
     9961 tgagggtgat gtaccagcgg gtgaacttac cgaacagacg cagaaatgtt ttcatgtgcg
    10021 gtcctttgca ataagccagt acagcagccc cgccagtatc gggtcgataa tcagaccgag
    10081 aatgaaccag ccccagaacg agcgtccatt agcggaagcc acgataccca gcaacagggc
    10141 cagaccgccc cataacagaa ttaacggcat atactttcct taatgcgtga caatccctgc
    10201 gatacatcgc attgattttg atggttaaaa ttaacaaatc acataatgcc ctgtggatta
    10261 tgtggctacc ggattaatca tgtacaacga tgaatatcga tcggctcgat cggctcgatc
    10321 gacaaaatcg atcgtaatga aacaaagaaa cttatcgaaa acggcaaggc cgctcatcga
    10381 aagcgaagtg actagctgaa gaaaaatccc cacagggagc ctgccgcgcg ggcgacggtg
    10441 tgggtcaccg catgcagtgc tgtgcgggcc acctgcggca gcggtgcgag gtcaatgatg
    10501 ttatcaatca cctctaccac ggcatcgcca aagctgctgc tggcggtatt gataacggta
    10561 tcagtgcgat accggttctg cagatgaggc acaagtggac tgcgggcttg ctttgggagg
    10621 cgggtaatca tggtctccac caggcgcggc agccggtaac gcccccggac agagactggc
    10681 agaatatcta tttctgctat ctgcaactga cgtgccacag cctgccgttt ctgcgcaata
    10741 cgctccgcct gtaacgccga gggttgagcc gacaaccatc gccactgctc ggcaggttcg
    10801 gttttgtcca cttggttgag tatccacagc actggcggta tcggtcctct gaactgcgcg
    10861 aacacgtcct gataaaattg ctcttccacc gcaaatgcgc ggtcatcggc cttgagcacc
    10921 cacagcacca tatcgagctg tggcatgagg tcacgataca gctcacggta ttcaccgtcg
    10981 cgggtaacag actcgcccac tccgggcaag tcgaccagcg tcagataatg gctgccgaag
    11041 cgcagccgga cacgctgtgg ctgacgggtg caggcttcca cggcattgac agtacagact
    11101 tcgctccgga acagtgcatt gcacagcgag gatttgccga cgccggtttt tcccatgatg
    11161 ccaatcaccg gttcatacgt aacgagtcgg ttgagctcct gcagcaaatg ctggcgtagc
    11221 gcgtgtggat aacgccgcag atggcgacgg ataagggtat aacccgaggg atgtttcata
    11281 tcggcctctg aatgaaaaaa ggccatccgg tggggatgac ctgttggatt aaaacgggtg
    11341 gaaattcact gaggttatat atatctgaga attatttaaa tcatattacc tggcagtata
    11401 tctccgtact gacattactc gtctgtgttt ttataaagaa agcacaggcg aatgaaaata
    11461 ccagaagctg ccacttgact aatttagtcc aacgctgtct gactagacta aatatcagtt
    11521 tttcatattg acttgcgatc gcattccaga ttagttttca attatcaaaa caacattttc
    11581 tggaggtatt gtcatgcagg agtttccatt taacgctttc ccctcgtcat tgcttgaaac
    11641 tatttttgat attgaggaat gcacacgagc cccgggggaa atgattgcat taacattgct
    11701 gagtggatta tcactggcgt gtcagagcct gattaatgta aaaataacag acaccatgaa
    11761 gtctccggtg tcattgtatt cattcgtgat agcagattct ggcgagagaa aaacagcggt
    11821 ggaaaaactg ctgttaaaac cttttcatga gcatgatctg cgtgtattac tccagtatga
    11881 aaagcagaag aaagaacatg aggtccagtc taaaatatgg agcaagaaag aaaatgcact
    11941 tctgaaatgc attgataaag acactatgaa aggactatgt acagataaac tttcacggca
    12001 gttagaacag ttatctgctg aaaagccagt acaaccgaaa gccaaaaggt atatttataa
    12061 taatgtcacc cctgaagccc tgcagtttga aatgtatacc cactcgccac aaacaggact
    12121 aataactgat gagggggcta atatacttag cagacggact atgagtgatt tgggatttat
    12181 gaactccata tgggatgatg aaagttttca tgttaatcgg aaaacagggc caagttttac
    12241 gattaagaat ggacgtatta cgttgttagt catggtacaa aaagcaatat tttacgattt
    12301 tttaaaacgc aagggcgaac atgcacgcgg gagtggtttg tttgcacgct gttttatctt
    12361 gtatattgat gcgaaattat cgacacaggg tcagcgtttt attagtcgtc tatcttcccc
    12421 tcagtcaggt aagctggaga atcttaatcg cttctaccaa cgtatgaatg aactgataga
    12481 aaaaagtgct tctcaacacg ggactgggaa tcagacatgt ttatcattcg agccctcagc
    12541 cttggcggcg tggaaagaaa ttcacgatga aatagaaagc agtattggcc ccgacaggga
    12601 atatgcaaac atgaatgatt ttgcatccaa acttccgaat aatgttgcac gtctggccgc
    12661 gctgctgagt tactatactg aaggtgaatg cgcgataaaa aaagaatatg tcgaaaatgc
    12721 ctggttgctg tgtgaatggt acatgcaaca ggcaataaag gtctttgggg ctcaggaggg
    12781 ctactatgaa gcgctgctat tatcatggct tcgtcgtgag tactatgaaa cagagatgga
    12841 tcatgtcaga tttaatacaa tacgaaatgc cgggcctaat gtattacgta aaggcaagtt
    12901 actggaaaga gttattgaga gacttgaaaa ggaaggcgcg atagacatcg tccgctcaag
    12961 acgcaaggca cgaatggttt atgcaggccg ttattttcgc aacaatccgt ttacaaaaaa
    13021 tgcagcatat aagcactact ggccgcgata gttgcgactg atcgcaacta atcgcgaggt
    13081 ttgcctgttg aaatacccaa aattgttgaa aactaatatc agagtgaaat tattatatga
    13141 cttaccattt ttcgtaaggt ttactcagga ggagggactt attgttatat tttcactgat
    13201 gaaattactg tcggtatagt aatatatgtt tattagtaat atatacctgt tactggtgta
    13261 ca
//
LOCUS       DAZUCE010000133        13206 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZUCE010000133 DAZUCE010000000
VERSION     DAZUCE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.133"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..333
                     /locus_tag="ACHY03_003284"
     CDS             <1..333
                     /locus_tag="ACHY03_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFS3906477.1"
                     /translation="KNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLILGLELPYLS
                     MLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEGTSGSNRYG
                     NDPKFGSN"
     gene            complement(384..1280)
                     /gene="yhaJ"
                     /locus_tag="ACHY03_003285"
     CDS             complement(384..1280)
                     /gene="yhaJ"
                     /locus_tag="ACHY03_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFS3906478.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1385..2086
                     /locus_tag="ACHY03_003286"
     CDS             1385..2086
                     /locus_tag="ACHY03_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFS3906479.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGSMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2109..2273
                     /gene="yhaL"
                     /locus_tag="ACHY03_003287"
     CDS             2109..2273
                     /gene="yhaL"
                     /locus_tag="ACHY03_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFS3906480.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2407..3717)
                     /gene="cyuA"
                     /locus_tag="ACHY03_003288"
     CDS             complement(2407..3717)
                     /gene="cyuA"
                     /locus_tag="ACHY03_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFS3906481.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3745..5076)
                     /locus_tag="ACHY03_003289"
     CDS             complement(3745..5076)
                     /locus_tag="ACHY03_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3906482.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5351..6715)
                     /gene="tdcG"
                     /locus_tag="ACHY03_003290"
     CDS             complement(5351..6715)
                     /gene="tdcG"
                     /locus_tag="ACHY03_003290"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFS3906483.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6787..7176)
                     /locus_tag="ACHY03_003291"
     CDS             complement(6787..7176)
                     /locus_tag="ACHY03_003291"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFS3906484.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7190..9484)
                     /gene="tdcE"
                     /locus_tag="ACHY03_003292"
     CDS             complement(7190..9484)
                     /gene="tdcE"
                     /locus_tag="ACHY03_003292"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFS3906485.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9518..10726)
                     /gene="tdcD"
                     /locus_tag="ACHY03_003293"
     CDS             complement(9518..10726)
                     /gene="tdcD"
                     /locus_tag="ACHY03_003293"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFS3906486.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLV
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10752..12083)
                     /gene="tdcC"
                     /locus_tag="ACHY03_003294"
     CDS             complement(10752..12083)
                     /gene="tdcC"
                     /locus_tag="ACHY03_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFS3906487.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12105..13094)
                     /gene="tdcB"
                     /locus_tag="ACHY03_003295"
     CDS             complement(12105..13094)
                     /gene="tdcB"
                     /locus_tag="ACHY03_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFS3906488.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVARPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3360 a         3600 c         3137 g         3109 t
ORIGIN      
        1 aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag agtactggat gtttactctg
       61 attaacgcca tcgtcggcgc tattatcaat gtcattcaat tgattttagg tctggagctt
      121 ccatatctgt ctatgcttta tttgctggca accttccttc ccgtccttgc gcttgctatc
      181 cgtcgcttac acgataccga ccgttcaggt gcctgggcct tgctcttttt tgttccgttt
      241 attggctggc tcgtgcttct ggtttttttc tgcacagaag gtacttctgg cagcaatcgt
      301 tacggaaacg atccgaagtt tggttcaaat taatcttaga attggggcga tatttcgccc
      361 ctttttatta acaataacaa atattatttt ccgctaaaaa gtttgggaat ttcccgcaga
      421 caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat atcgatctcg
      481 ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt ttcgaccatc
      541 ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt atcttcaatc
      601 gtgctcaccg ttaagcgcgg ctgtttgtcc agcagctgta cggtcaacac cgggcgctca
      661 cgagcggtat ccgccaccgc aattccacga tatttcacac gcgtcacttc agataacggt
      721 tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat taacgtatag
      781 agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc gataacaata
      841 tccgcccgcc cctgctccag ccgttcccac gcccccgcca gcacttctgt gatgattgcc
      901 agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt
      961 accagcgctt cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag agcttccgca
     1021 tcagtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccaa caacatcctc
     1081 ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac atccagctct
     1141 tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag ctcatccgct
     1201 gccgccgcaa aactgccccg gcgatcgatc gcatccataa cccgtagtgc ttccagcgtt
     1261 aatgcccttt ctttggccat ttcgttctca ttcaggaaat ttgaacatac cgggcagaat
     1321 atctggctaa caatgtagcg tccagcccct taccataaaa ggaagtaaag agaggtcaag
     1381 aattatgatt actacccgaa ctgccaggca gtgtggacaa gcagactacg gatggttgca
     1441 ggcccggtat actttttcct ttggacacta cttcgacccg aaattgttag gctatgcctc
     1501 cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc gcctttcagc cgcgaaccta
     1561 tcccaaagtc gatattttaa atgtgattct ggatggagaa gcagagtatc gcgacagcga
     1621 aggcaatcat gttcaggcca gcgccggtga ggcgttgctg ctctctaccc agccgggtgt
     1681 cagctacagc gaacacaatc tcagcaaaga caaaccgtta acacgaatgc agctttggct
     1741 ggacgcctgc ccgcagcgag agaatccgct gattcaaaaa ctggcgctta atatgggcaa
     1801 gcagcaatta atcgcctcgc cagagggttc gatgggaagc ctgcaattac gccagcaagt
     1861 gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg aatttccagt tgcatggacc
     1921 acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg cttacgcatc atgaagagaa
     1981 agcggcgctg acctgcggtg atggggcgtt tattcgtgac gaggctaaca ttacgctggt
     2041 tgccgattcc ccactgcgcg ctttgctgat agatttgcct gtctagttgt ttttacaggg
     2101 agatgatgat gagtaaaaaa tcggccaaaa agcgccagcc agtgaagccc gtggtggcga
     2161 aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga gatgttgagc gagctggaag
     2221 ccatcgtcgc ggatgctgaa acgcgtttag ccgaggatga agctaccgcg taaagacagg
     2281 ttgccggatg cggcgaaaac gccttatccg gcctacaaaa tccaacaaca ttaacgaatt
     2341 aaacaacccg taggccggat aagatgcgcc agcatcgcat ccggcacgat acccaaaacc
     2401 tggcgtttat ctggccttgc tcgccataat ctcgataatc tgccgatccg tttgctgcat
     2461 cgaatggctt gctaatgcac acaggttggc aatcgactgc tcaacatcat gcgccacaat
     2521 cccttcattg ccggtcacgg cggtatcatc cagcgccatt aacaccgctt tccacgcagc
     2581 cgaagcactg gtcgaaacct tcatcgcgca gctgttcgac gcaccatcgc aaatcatgcc
     2641 gctgacatcg ccgatcatac tgctgatcgc catcgagatg gtttcataac gcccatccac
     2701 cagccatgcc atcccggcgg cagcccccat tgctgcggtc gttgcggcac acagcgcaga
     2761 caaacgcggt aactggttat ggatgtaaat tgcgctcaaa tgcgaaagca tcagcgcacg
     2821 cgccagccgt tcatcatccg ctccgaagtg ttctgctacc accaccacag gcattgttgc
     2881 ggtgatcccc tggttacccg agccggagtt actcatagcc ggaagcgtag cgccgcccat
     2941 acgcgcatcg gatgccgcgc tggtacgaat cacaatggat gaagagagat ctttcgccag
     3001 caaaccgcgc gcgcactgtt tttccagcgt cgcgccaata tgcagccccc acttaccgct
     3061 caaaccttcc tgcgataacg cacaatttag cttcgcagaa tcgagaataa agcggatcgc
     3121 cgcaaacggg acttcattga cgaacttcag gatctcagcc agcgtcgttc tggaaagcac
     3181 cgtcagcgga gactcttgct cgccctctgc cacacacgcc tgctgggtaa acaccacacc
     3241 atcgtgcgtc tcaatatgca caatgttggt atgcccgccg acgatagtga cacacgccca
     3301 tttctcaccg ttccagactt tggcgcgtga gaagaggatt tcattgcaag gttcctggat
     3361 cttaacggag actttccccg ccgccagcag tgctttggca tcggcaattg cctgcgcagt
     3421 tgcgtctttc agcacttcca gcccggcgtt ggcatttcca cctaacgccc ccagcgccgc
     3481 cgcaatcggc agccccacca ttcccgtgcc gggaacggtg acgcccagac cgttcttcat
     3541 cagatttggc gaaacccagg cttctacacg ttcaaccgga ccttccagtt ctgccgcagc
     3601 aaccgccgcc gccagcgcca gtgaaatcgg ttcagtacat cccagcgccg gttttacttc
     3661 ctcctgaacg gcgaggatgt aacgctgcca taacggattt aaagtcgaat caaacatatt
     3721 aaaaacctta aaatttcagg taaatcaaga aaatgccagg aacggagaaa cacaaagcaa
     3781 caaaccagtg acgataatca ggtacagaga catccctttg tatttgtgca atgccggtac
     3841 tttgtaaacc agccacgccg ggatcaggca ccctaccatg ccgaaaatcg ggctacagat
     3901 agaggtgaag ctcaacaccg gtgcgttcag tacgatggcg ctccaggcca gcaaaatggc
     3961 gaaaatcatg atgccgcgct gaacaagatt ttcgttaatc ttctcggcag gcatcttgcg
     4021 acgcaggatg ttcattacga tcccttgcgt tgcttcgcga aagcctaaat agacgccaaa
     4081 gaatgcagtc atgacagcaa agatattgag aatgacgctg accactttca cccacgctgc
     4141 gccgtcaccg ctaataaact gtgcggcaat cgccagcgcg gaaatattct gctcgtaggc
     4201 ttttaccgct tcgtcatgtc ccatcgccag cgtgaacgac acggcgtaga aaaagacggt
     4261 gacaaacaaa atgccaaacg cgatattcat tgcccgcaat gctttatgcc gcgccacttc
     4321 aatcgatttt tcccgcgagc gataagagat caccatcggg cttaacgtct ggataaacag
     4381 aatcgacgtc agggtaaacg gcagcgtaat aatggcgttt ttcaccagca gccccagcgg
     4441 cggtagcgaa ccgacgttat acagatgcca cattcccacc atcgacacgc ccagcgccgc
     4501 gaccaccagc agcttggtca gcaccatgcc agtcgaaatt ttgaataaca atttctcgcc
     4561 gcgtgaagag atcgccacca gaatgcaaat cagtaccaga ccataaaagg gactgtctga
     4621 cagcaaccct tccgtcacgc cgaaggtatg caggtaggaa gcactatcgt tggtgatggc
     4681 ggtggaatag acgaacatcc aaatcaccag cattacgaaa tagagtgcac ctaacaggat
     4741 gccccagttt ttacctaaat aaccgctaat gacgctcggg taatctttac attctggtga
     4801 ttctgccagc gtattaataa acaaccgctg aaacagatac attgccgggt aaccaatcac
     4861 cgatgagagc aaaaataccc acaatcccat caaaccgacc tgcaccggga gaaaaacaat
     4921 ccccgcgcca atcgccatcc cgatactcat aatcacccag ccggtgtcag tactgtcgaa
     4981 tttgatcgct tctcgccact cgctctcact cattccagca cgacccgccg gggtcgaagc
     5041 gtctgcaatg acgcctttat tcgatgcaat ttccataatt tctcgctcaa tattttgtag
     5101 ggcttattat ttttttccga gccgcatcaa ggcgatatgc ggtctacgtt tttgcaggcg
     5161 agttattaga ataaagaaat gatacgcgcg aggtcagaga aaatcttcac aatcaagact
     5221 ttgaaataac ggagatggat aagaattttc tactaaatta atcgcaagag aatattcatt
     5281 ctctatttgc ggcagatcac aaaaaaaagg tgcacatttg tgcacccaag gatgaaagct
     5341 gacagcaatg tcagccgcag accactttaa tggccagtcc tccgcgtgat gtttcgcggt
     5401 atttatcgtt catatctttg ccggtttcat acatcgtctc gatcacttta tcgagtgaaa
     5461 cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct ttcacggcat
     5521 taatggcatt acgttcaatg cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca
     5581 gtccaaggtt atgctccatc gcgatttccg ccgcattgca tacctgcgcc ggactgccgc
     5641 ccagtagttc agttaaccct gccgccgcca ttgaacaggc cacgccaatc tccccctgac
     5701 agccgacctc cgcgccagag atggaggcgt tcattttata cagcgcgcca atagccccgg
     5761 ctgcaagaaa atagcgggca attgaccgct cgtttaccgg acgacggaac ttatcgtaat
     5821 aagccagcac tgccggaata atgccgcacg caccgttagt cggtgccgtt accacgcgcc
     5881 cgccagctgc gttttcttca ctaaccgcca gcgcgtacat gttgatccag tcgatgacat
     5941 tcatcggatc gttagagatg ttatcgctgg aaaccagctg acgacgcagc gctacggcac
     6001 ggcgcggcac attgagcgga ccaggcagca cgccttcagt gttcatcccg cgttcaatac
     6061 cgtcatgcat cacttgccag atacgggcaa aaccggcgtc aatttccgct ttactgcgca
     6121 gcgctagctc gttgtgcatc atcaggccag aaatagacag gccgttgtaa tcacacattt
     6181 tcagcagttc acctgctgag tggaaatcgt aaggtacgga cgtttcgaca tcgtgcgaca
     6241 ggccgaagtg ttcttcttcg acaataaacc cgccgccgac agagtaatag gttttactta
     6301 atagctcttc ctgtgccttc caggcagtga tccgcattcc gttctcatgg cgaggcaaca
     6361 tttcgggatg gaagataatg ttctttgcta caggaaaatc aacaatatgc gcaccagatg
     6421 ccactggcag ccgcccgctg cgcgttacta actctataaa tgcagggatc tcatcaatga
     6481 caacatcctg cggactgttt cctgccagtc ccatgatgat ggcgacatcc gtggcatggc
     6541 ctttgcccgt cagtgacaac gacccgtaca gatcgaccac aatatggctc gtcgcggtta
     6601 ataagccgct actttccagc cgatcaataa aactttttcc ggcattcatt ggccccacgg
     6661 tatgcgaact ggagggacca atcccaattt tgaaaatatc gaatgcacta atcatatcca
     6721 caccctcgga ttgccgttca gtgaagtgga gcggaacgac cttacgaccg tcccgctcac
     6781 gaggctttac gcactacgta ctgcgatagc ttcaatttcc agcttcacat ctttcggcaa
     6841 acgcgcgacc tgtacacagc tccgggtcgg gtaggtcgcc tgatgctcat cgaagaactg
     6901 cttatagact tcgttgatgg tggcaaaatc attcagatcg gtgatgaaca cggtcatctt
     6961 gatgatatcg cccacgctca gcccggcagc aaccacgatc gctttgacgt tttcgaggct
     7021 taaacgcgcc tgatcttgca catcagccgg gatctcaccg gtctgtgggc aaaccggaat
     7081 ttgaccggag gtgaagacca tgctgcctaa atcaacgccc tgaacataag ggccgattgc
     7141 gcctggggca cgttgcgttt cgataatctt tttcatacat cctccggcgt cagagcgcct
     7201 gggtaaaggt acgtgaaata acatcctgct gctgttcacg ggtcagtgcg ttgaagcgca
     7261 cggcgtagcc agagacacgg attgtcaggt taggatattt ttccgggtgc tcgatggcat
     7321 ccagcagcat ttcccgattc attacgttga cgttgaggtg ttgaccgcct tcgacatccg
     7381 cttcgtggtg gaaataccca tccagcaggc cgacaaggtt ggttttacgt actggatctt
     7441 ctttgcccag cgccgcaggg acgattgaga aggtgtacga gatcccatct ttggcgtagg
     7501 tgaacggcag tttcgccacc gacgtcaatg aggccacggc acctttgcgg tcacgaccat
     7561 gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg acgaccgtcc ggcgtgttac
     7621 cggttttctg gccgtacacc acgttagaag tgatagtcag aatcgactgg gtagggacgg
     7681 cgttgcgata ggttggcagc gctttaattt tcttcataaa acgttcaacc aggtcgcagg
     7741 caatgctgtc tacgcgctcg tcgttgttgc cgtactgcgg atattcaccg tcgatttcaa
     7801 agtccaccgc caggccgttt tcgtcacgga ttggtttcac gcgggcatat ttgatcgcag
     7861 acagggagtc cgttgccacc gacaggcccg cgatgccgca tgccatagtg cgatagacat
     7921 cacgatcgtg cagcgccatc agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa
     7981 tgatattcag cgcgctgatg tactgcaccg ccagccagtc catgaagtga tcgaggctgt
     8041 ccatcacttt gtcgtaatcc agcacatcgt ccatcaacgg tgctgttttc ggcccgacct
     8101 gaatcttcag cttctcgtcc accccgccgt taattgcgta gagcagcgtt ttcgccaggt
     8161 tagcgcgtgc accaaagaac tgcatttgct taccaatcac cattgggctg acgcagcagg
     8221 caatcgcgta atcgtcgctg ttgaagtcag tacgcatcag atcgtcgttt tcatattgca
     8281 aggaagaggt gacgatcgac acctgcgcgg catatttttt gaaggcaatc ggtaattctt
     8341 ccgaccaaag aatggtcagg ttaggttccg gtgcaggccc catagtgtgc agggtgtgca
     8401 aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc cagccccatc ccgccgatca
     8461 cttccgtcgc ccagattggg tcgccggaga acagcgaatc aaattccggt gtacgcagga
     8521 agcgcaccat acggatcttc atgatgaagt gatcgatcag ttcctgtgcc tgctgctcat
     8581 tgagtacgcc agctttaaag tcgcgctcaa tgtagatatc caggaacgat gccgtgcgac
     8641 ccagcgacat cgcgccgccg ttctgtgatt tcactgccgc cagataagcg aagtagagcc
     8701 actgcaccgc ttcctgcgca ttctgcgccg ggcgagagat atcaaagcca tatttcgccg
     8761 ccatttcctg aatctgcaac agcgcatgac gatgctctgc cagctcctca cgcagacgga
     8821 tggtggcttc cagatcctcg cctttttcca gacgagactg gagatcggca aattgcagtt
     8881 cgcgttcacg caccagataa cgaatgccat acagcgctac gcggcgatag tcacctataa
     8941 tgcgcccacg tccataacca tctggtaaac cggtcagtac gccagattta cggcagcgca
     9001 gcatatccgg tgagtaaaca tcaaatacgc cctggttatg ggttttacgc agatcggtaa
     9061 acagatattc aaattcactg tccatttcac ggccataggc gtggaatgaa cttttaatca
     9121 tattaatgcc accgaacggg tgtagcgcac gtttcaacgg cgcatccgtt tgcaggccaa
     9181 caattttttc cagcggctgg ttaatatatc ccgcatcatg agcggtaatt gtggtggcaa
     9241 tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc aatacgaatg ccttccatca
     9301 ccttttccca caattccgtg gtggcaggcg tcgcttcggc gagaaaagat tcatcgcctt
     9361 catacggtgt atagttatgt tgaataaaat cgcggacatt aatttcgctt ttccagtccg
     9421 tacctttaaa gccaagccat gcgtcggcgt acagcttatc gctggtatca atatctacct
     9481 tcatgaaaaa taatctctct acaatacttc aactaaatta tgcaaattct gcgggcgcgt
     9541 taactttgcc taaatgaatg gcatccaaag caatcatttt ttcttcgtta gtcggaataa
     9601 cggcgcaaat gacacgcgca ttttcactgg aaacaattcg ctcgccaaaa gagttagatc
     9661 gattattcat ttctgtatca atctctacgc ctaatacagc caaatgttcc atgaccagac
     9721 gacgaactaa acttgaattc tctcctattc cgccggtgaa tataattcca tccaggcgac
     9781 gtaatgaagc tgcgtgtccg gcaatatgac gggcaattcg gtgaacaaag gttttaattg
     9841 ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc cagaacacgt aaatccgaag
     9901 aaagaccgga aatacctaat aatcccgact ctttattcac tacgcgttcc aggtcaccca
     9961 ggctctggtt ggtttggctg gcgacccagg acatcgcacc aaagtcgaca tcgccactgc
    10021 gggtacccat catcaagcct tccagcggcg tcattcccat tgaggtgtca acactctgac
    10081 cgttgcgaac tgcgcagatt gacgcgccat tgccaagatg cgccacaacc aggccggagt
    10141 catcttccgc cagattcagc aaagaatgtg cgcgctggga aacatagcgg tgcgacgtgc
    10201 cgtggaaacc ataacggcgt acacctaact cttcataata tttccacggc aggccgtata
    10261 aataagcttc cggagccatc gtctggtgga aactggtatc aaataccgcc acctgagtta
    10321 cgcccggaaa taattgctgc gccgattcaa taccacttaa attggcgtaa ttatgcaggg
    10381 gtgccagtgg agaaacgcga cggatattat caatgacttc atcggtaata atggcggact
    10441 cggtaaaaat actgccgccg tgagcgatgc ggtggccaat taaggccacg ctgtcattta
    10501 aattccgttt ttccagttca aatgcaattg ccttcaatgc accttcgtag ctgtggtgag
    10561 ccagcggtgc tggctctccc ccatttacgg ataagaatgc attttccgag ttaataccgt
    10621 cggcaatacc tgacattaat acttcacagt cgctggcatc gagtacggaa aacttaatcg
    10681 aagacgaacc acagttaata accaaaacaa ccggaaattc attcatctct tttctcatcc
    10741 tgagttacgg attaaaacag tttgtatacg atgttcagga tggtcagcag accaatcacg
    10801 gtaacaaaca cgttatccag acgaccacgg tatttcgcca gagacggcgc tttacggatg
    10861 gcatacatcg gcaacaggca cagcagggat gcgataatcg gtgcgcccat ggcttcaatc
    10921 aggtcgagga tgttcgggtt ggcgtaggca acaacccagg tggagcccat gatgaagatc
    10981 atgctgagag tattcagttt acccagcgac actttggttt tgtcaccttt ataaccgaac
    11041 ttcagaatca gaccattcaa gccttccagc gtccccagat agtgaccgaa gaaagatttg
    11101 aagatagcca cgagtgcgat gatggaagcc gcatattcca gtgtaatcgc gaacgttgtt
    11161 ttcgtaccgg tcatggacgc aaagtggtta gccagataag aaagcactgg aatattctgc
    11221 gctttggctt ccgccatgtt ggccggagac agagtaaaca ggcagctaaa ggcaaagaac
    11281 atcaccactg caaccatcag catgctggca cgagaaatga tttgggaaca tttacgttcg
    11341 gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt tagaaaccac gaaggaagag
    11401 acgattggcg agaagttaaa ggagaaaacc atgatggaaa tccccagcca gacagtgatc
    11461 aggataccgt catgaccggt taacgacagc gaaccgaggt caacctggtc gataactgca
    11521 gagttccagt aagggatcag cgacaaagaa atcagcacca ggctggcgat aaacggccat
    11581 accaggtagc tcatcacttt aaccatcaga tccttaccaa accagatgac gaaagccatc
    11641 agcagcaaca ggaacagcgc cacaaagccg cgattcagcg gtgcaaagcc gagctggttt
    11701 tcccagaacg tcataaaggt attggtaata gtaacgccat aaatccacag cagtgggcaa
    11761 atcgcgaaga agtacaggaa cgtgataacc acgccgccag ttttaccaaa atgctcttcc
    11821 accgtttccg taatgttgcc ggaaggatta gagccagaaa gacacagacg cgccagcgcc
    11881 cggtggcaat aaaacgcgat ggggtatgcc aataccaaca tcagaagaat cgggatcagt
    11941 ccgccaaaac ctgcgcggat agggaagaac agcaccccgg cgccgattgc cgtaccaaac
    12001 aagcctaacg tccatgtggt atctgattta cgccaggacg attgttttgt ctggctggat
    12061 acaatgctat ctgaagtact catatcctat cctcaacgaa ttaattaagc gtcaacgaaa
    12121 ccggtgattt gagagacgcg agaaagatcg atattgccgc cggaaataat actgacggtt
    12181 tttctgtttt gaatatattg gtctaattta ccgcttaata atgcagcaca tgccagagcg
    12241 cctgcgcctt cggtgacgac tttattgcgc tgaattaagg caatcatact gtttctgatt
    12301 tcgtcttcgc tgaccagcac gatgtcatcg actaattcac gaacgatttc gtaagttaaa
    12361 ttacccgggc gggcgacatc acaaccatcc gccagggtgc cggtagttcg gtgcgtggtt
    12421 atttctccgg agtggaaaga agccgccatg ccgtgaacgt tttcagactg tacgccaata
    12481 acacgaatgg tcgggttaat agatttaatt gccaccgcaa taccagcaat taaaccgcca
    12541 ccaccaattg gcacaatcac gttatcgaca tcatagagat cttccataat ttccagacca
    12601 atcgttccct ggccagcaat cactttcgga tcatcgtaag gtgggataaa aatacggcct
    12661 tccatttcga caatttcgct cactttagcg atagtgtcgt tgaagttatc accatgcaga
    12721 acgacttctg cagagtagtc gcacgttgcc gctactttgg attttggcgc gccttttggc
    12781 atcaccactt taccgtcgat acccagcatc gcgcaggaga gcgaaacccc ttgcgcatgg
    12841 ttgcccgcag aacaggccac cacgcctttg cgtttttccg catcagtcag tgaacttaat
    12901 ttattaaatg cgccacgaat tttaaatgaa cccgtacgct gcatattttc aaacttcagg
    12961 aatatttcac ctttgcaacg ttcactaaaa tagttggagc gaggcatgcc tgttttataa
    13021 attcgcccag ccagtcgttg tttcgcttca ataatgtcat caatagcaac cggcagatcg
    13081 tatgtaatat gcattataaa acctcttcgc ctgaattaaa tatgtaggta accgtaaccg
    13141 acacctgcaa gacagacagg tggattattt atgtttaaat aaaaacaaat aactaaccaa
    13201 cttcta
//
LOCUS       DAZUCE010000134        13149 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZUCE010000134 DAZUCE010000000
VERSION     DAZUCE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.134"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            430..2061
                     /gene="oppA"
                     /locus_tag="ACHY03_003296"
     CDS             430..2061
                     /gene="oppA"
                     /locus_tag="ACHY03_003296"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFS3906489.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTYDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2147..3067
                     /gene="oppB"
                     /locus_tag="ACHY03_003297"
     CDS             2147..3067
                     /gene="oppB"
                     /locus_tag="ACHY03_003297"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFS3906490.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3082..3990
                     /gene="oppC"
                     /locus_tag="ACHY03_003298"
     CDS             3082..3990
                     /gene="oppC"
                     /locus_tag="ACHY03_003298"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFS3906491.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4002..5015
                     /gene="oppD"
                     /locus_tag="ACHY03_003299"
     CDS             4002..5015
                     /gene="oppD"
                     /locus_tag="ACHY03_003299"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFS3906492.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5012..6016
                     /gene="oppF"
                     /locus_tag="ACHY03_003300"
     CDS             5012..6016
                     /gene="oppF"
                     /locus_tag="ACHY03_003300"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFS3906493.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6069..6398)
                     /gene="yciU"
                     /locus_tag="ACHY03_003301"
     CDS             complement(6069..6398)
                     /gene="yciU"
                     /locus_tag="ACHY03_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFS3906494.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6433..7893)
                     /gene="cls"
                     /locus_tag="ACHY03_003302"
     CDS             complement(6433..7893)
                     /gene="cls"
                     /locus_tag="ACHY03_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFS3906495.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8036..8209
                     /gene="yciY"
                     /locus_tag="ACHY03_003303"
     CDS             8036..8209
                     /gene="yciY"
                     /locus_tag="ACHY03_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFS3906496.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8263..9516)
                     /gene="kch"
                     /locus_tag="ACHY03_003304"
     CDS             complement(8263..9516)
                     /gene="kch"
                     /locus_tag="ACHY03_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFS3906497.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(9816..10112)
                     /gene="yciI"
                     /locus_tag="ACHY03_003305"
     CDS             complement(9816..10112)
                     /gene="yciI"
                     /locus_tag="ACHY03_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFS3906498.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10336..11055
                     /gene="tonB"
                     /locus_tag="ACHY03_003306"
     CDS             10336..11055
                     /gene="tonB"
                     /locus_tag="ACHY03_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFS3906499.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(11095..11493)
                     /gene="yciA"
                     /locus_tag="ACHY03_003307"
     CDS             complement(11095..11493)
                     /gene="yciA"
                     /locus_tag="ACHY03_003307"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFS3906500.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11598..12137)
                     /gene="yciB"
                     /locus_tag="ACHY03_003308"
     CDS             complement(11598..12137)
                     /gene="yciB"
                     /locus_tag="ACHY03_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFS3906501.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12167..12910)
                     /gene="yciC"
                     /locus_tag="ACHY03_003309"
     CDS             complement(12167..12910)
                     /gene="yciC"
                     /locus_tag="ACHY03_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFS3906502.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(12938..13027)
                     /locus_tag="ACHY03_003310"
                     /pseudo
     CDS             complement(12938..13027)
                     /locus_tag="ACHY03_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT         3541 a         3123 c         3228 g         3257 t
ORIGIN      
        1 aagatagaca tgcttaacaa atctctattt gattgaattt aaagatgttt gttaagacat
       61 ggatgcaagc tatagattct gatacggtca ataaaagaga attgcttaac aattttgcaa
      121 aatgtattgg cgagtaagaa gcgcatttgg tactttccgg gcaaccgcca ggcgattctt
      181 tattggtaat gagaataatt aacaattaaa gagcgtcgtg aaagaataat gtgtctcgac
      241 aggggagaca cagtacgaat cgacataagg tgatcgtctg aatcaccaga ataaataaag
      301 tcggtgatag caatccgtaa cgataaagta acctgacagc agaaagtctc cgagcctgtg
      361 cagggtccca atccgggatt acacatgctg gttaatacca gtaattataa tgagggagtc
      421 caaaaaacaa tgaccaacat caccaagaga agtttagtag cagctggcgt tctggctgcg
      481 ctaatggcag ggaatgtcgc gctggcagct gatgtacccg caggcgtcac actggcggaa
      541 aaacaaacgc tggtacgtaa caatggttca gaagttcagt cattagatcc gcacaaaatt
      601 gaaggtgttc cggagtctaa tatcagccga gacctgtttg aaggcttact ggtcagcgat
      661 cttgacggtc atccagcacc tggcgtcgct gaatcctggg ataataaaga cgcgaaagtc
      721 tggaccttcc atttgcgtaa agatgcgaaa tggtctgatg gcacgccagt cacagcacaa
      781 gactttgtgt atagctggca acgttctgtt gatccgaaca ctgcttctcc gtatgccagt
      841 tatctgcaat atgggcatat cgccggtatt gatgaaattc ttgaagggaa aaaaccgatt
      901 accgatctcg gcgtgaaagc tattgatgat cacacattag aagtcacctt aagtgaaccc
      961 gttccgtact tctataaatt acttgttcac ccatcaactt caccggtgcc aaaagccgct
     1021 atcgagaaat tcggcgaaaa atggacccag cctggtaata tcgtcaccaa cggtgcctat
     1081 accttaaaag attgggtcgt aaacgaacga atcgttcttg aacgcagccc gacctactgg
     1141 aacaacgcga aaaccgttat taaccaggta acctatttgc ctattgcttc tgaagttacc
     1201 gatgtcaacc gctaccgtag tggtgaaatc gacatgacgt ataacaacat gccgatcgaa
     1261 ttgttccaga agctgaaaaa agagatcccg gacgaagttc acgttgatcc atacctgtgc
     1321 acttactatt acgaaattaa caaccagaaa ccgccattca acgatgtgcg tgtgcgtacc
     1381 gcactgaaac taggtatgga ccgcgatatc attgttaata aagtgaaagc gcagggcgac
     1441 atgcccgcct atggttacac tccaccgtat actgatggcg caaaattgac tcagccggaa
     1501 tggtttggct ggagccagga aaaacgtaac gaagaagcga aaaaactgct ggctgaagcg
     1561 ggttataccg cagacaaacc gttgaccatc aacctgctgt ataacacctc cgatctgcat
     1621 aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa acattggtgt aaacgtcaaa
     1681 ctggttaacc aggagtggaa aacgttcctc gacacccgtc accagggtac ttatgatgtg
     1741 gcccgtgcag gctggtgtgc tgactacaac gaaccaactt ccttcctcaa caccatgctt
     1801 tcgaacagct cgatgaatac cgcgcattat aagagcccgg cctttgacag cattatggcg
     1861 gaaacgctga aagtgactga cgaggcgcag cgcacagctc tgtacactaa agcagaacaa
     1921 cagctggata aggattcggc cattgttcct gtttattact acgtgaatgc gcgtctggtg
     1981 aaaccgtggg ttggtggtta taccggcaaa gatccgctgg ataataccta cacccgaaat
     2041 atgtacattg tgaagcacta atggcaatac gtggggcagg agtgtcctgc tccacggtgt
     2101 ctgattttta tcgcattaca gaaggcacag gccagaaggt agggcaatgt taaaatttat
     2161 tctacgtcgc tgtctggaag cgattccgac gctatttatt cttattacta tttcgttctt
     2221 tatgatgcgc ctcgcgccgg gaagcccttt taccggcgaa cgtactttac caccggaagt
     2281 aatggccaat atcgaagcga aatatcatct taatgatccg atcatgacac agtatttcag
     2341 ctacctgaaa caactggcgc atggcgattt cggtccatcg tttaaatata aagattattc
     2401 ggtcaatgac ctggtggcat ccagttttcc cgtttctgcc aaactgggag ccgcagcatt
     2461 tttccttgcg gtaatactgg gtgttagtgc tggcgttatt gccgcattaa aacaaaacac
     2521 caaatgggac tataccgtga tggggctggc aatgaccggg gttgttatcc ccagttttgt
     2581 ggttgcgcca ttattagtca tgatatttgc gatcattttg cattggctgc cgggcggtgg
     2641 ctggaatggt ggggcgctta aattcatgat attgccaatg gtggcgttgt cactcgccta
     2701 tatcgccagt attgcgcgta ttacccgtgg ctctatgatt gaagtattac actccaattt
     2761 tattcgtact gcccgggcga aagggttacc tatgcggcgg atcattttac gtcacgcatt
     2821 aaaacctgct ctgttacccg tgctctctta tatgggcccc gcatttgtcg gcattattac
     2881 cggttctatg gttatcgaaa ccatttatgg tttgccgggg attggacaac tgttcgttaa
     2941 tggcgcattg aaccgtgact attccttagt gttaagcctg accatcctgg ttggtgcttt
     3001 aaccattttg tttaatgcca ttgtcgatgt gctatatgcg gttatcgacc cgaaaatccg
     3061 ttactgatac tggagctcgc gatgatgtta agtaagaaaa acagcgagac gctggaaaat
     3121 ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc aggacgcacg tcgacgtttt
     3181 atgcataacc gtgcggcggt tgccagtctg atagtgctgg tgctgatcgc gttatttgta
     3241 atcctggcac cgatgctttc gcagtttgcc tatgacgata ctgactgggc gatgatgtcc
     3301 agcgccccgg atatggagtc cggtcactac tttggtactg actcatccgg tcgcgacctg
     3361 cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg tcggtgttgc tgcggcactg
     3421 gtggcagtgg tcgtcgggac actttatggt tcgctttccg gttatctggg cggtaaggtg
     3481 gattcggtaa tgatgcgtct gctggaaatc ctcaactcct tcccattcat gttcttcgtc
     3541 attttgctgg tgaccttttt cggccaaaac atcctgctga ttttcgtggc gataggcatg
     3601 gtttcctggc tggatatggc tcgtattgtg cgtgggcaaa ccctgagtct gaagcgcaaa
     3661 gagtttattg aggcggcaca agttggcggt gtatcgacgc cgggcattgt tattcgccac
     3721 attgtgccga acgtactcgg tgtggtggtg gtctacgcat cgctactggt gcccagcatg
     3781 atcctctttg aatctttcct tagcttcctg gggttgggta cgcaagagcc gttaagcagc
     3841 tggggtgcat tgctgagtga tggcgcgaac tcgatggaag tctctccatg gttactgttg
     3901 ttcccagcgg gattcctcgt ggtgacgctg ttttgtttca actttatcgg cgatggcttg
     3961 cgtgatgccc tcgacccgaa agatcgttaa ggagtgcagc catgagcgta attgaaactg
     4021 caactgtgcc gctcgcacaa caacaggctg acgcactgct gaacgtgaaa gatttgcgtg
     4081 tcacctttag taccccggac ggcgacgtca cggcggtcaa tgatttaaat ttttccctac
     4141 gtgccggaga aacgctgggc attgtaggtg agtctggttc gggtaaatcg caaactgcat
     4201 ttgcgttgat gggcctgttg gctgccaacg gacgcattgg cggatcggca accttcaatg
     4261 ggcgtgaaat cctcaatttg ccagagcgtg aactcaataa actgcgcgct gaacaaatct
     4321 caatgatttt tcaggaccca atgacttcgt tgaatcccta tatgcgcgtc ggtgagcagt
     4381 tgatggaagt gctgatgctg cataagaaca tgagcaaagc tgaggcgttt gaagagtcgg
     4441 tgcggatgct cgatgcggta aaaatgccgg aagcgcgtaa acgcatgaaa atgtacccgc
     4501 acgaattttc tggcggcatg cgtcagcgag ttatgattgc gatggcattg ctatgtcgac
     4561 ctaagctgct gattgcggat gaacccacta ctgcgctgga cgtcaccgta caggcgcaga
     4621 tcatgacgct attgaatgaa ctgaagcggg aatttaatac cgccatcatt atgatcaccc
     4681 acgatcttgg cgtggtggcg gggatctgcg acaaagtgct ggtaatgtac gccgggcgca
     4741 cgatggaata tggcaacgcg cgcgatgtct tttatcaacc cgttcatcct tattctatcg
     4801 gtttgctcaa cgcggtgcct cgtctcgatg cggaaggtga aacaatgttg actattccgg
     4861 gtaatccgcc aaacctgctg cgattaccga aaggttgccc gttccagcca cgttgtccgc
     4921 atgcgatgga aatttgtagt agcgcaccgc cgctggaaga gtttacgcct ggccgtctgc
     4981 gtgcttgctt taaaccggtg gaggaactgt tatgaatgct gtaaccgaag gaagaaaagt
     5041 cctcctcgaa atcgccgatc tgaaagtgca ctttgaaatc aaagatggca aacagtggtt
     5101 ctggcaaccg ccgaaaacgc tcaaagccgt agatggtgta actcttcgcc tgtatgaagg
     5161 ggaaacatta ggtgtggtag gggaatcggg atgcggtaag tccacctttg ctcgcgccat
     5221 cattggcctg gttaaagcga ccgacggtca tgttgcctgg ttaggtaaag agttgctggg
     5281 catgaagccc gatgaatggc gtgccgttcg cagtgatatt cagatgattt tccaggatcc
     5341 gttggcatcg ctaaacccgc gtatgaccat cggcgagatt atcgctgaac cgctgcgtac
     5401 ctatcatccg aaaatgtcac gccaggaagt tcgcgagcgc gtgaaggcga tgatgctgaa
     5461 agtcgggtta ttgcctaacc tgattaaccg ctatccgcat gagttctccg gtgggcagtg
     5521 ccagcgtatc gggattgctc gtgctcttat tcttgaaccg aagctgatta tctgtgatga
     5581 gccggtgtcg gcgctggacg tgtcaattca ggcgcaggtg gtcaacctgc tccagcagtt
     5641 acaacgtgag atgggattgt cattaatttt tatcgctcac gacctggccg tggtaaaaca
     5701 catttccgat cgtgtgttag tgatgtatct cggccatgcg gtagaactgg ggacctatga
     5761 tgaggtctac cacaatccac tacatcctta caccagggca ttgatgtcgg cagtccccat
     5821 acctgatccg gatctggaga agaacaaaac catccagtta ctggaagggg aattaccgtc
     5881 gccgatcaac ccgccttccg gttgtgtttt ccgtacccgt tgcccgattg ccggtccaga
     5941 gtgcgccaaa acacgtcctg ttctggaggg gagtttcaga cacgccgttt cttgcctgaa
     6001 agtcgatccg ctttaaaaac aaaaagggct gacagctgtc agcccttttt cacgctaaaa
     6061 gcgattattt attcccgcca gatgatatgg caaagtttgt gatctttttc gcggcataac
     6121 agaatgcggg cgaaaacatc gttgatttca ccatcttcac tgtccgccag accaatcacc
     6181 acttcggcaa aaaagtccgg gttcaaatcg aaatcaacat gttcctgcca gtcttccgcc
     6241 ggatcgaata actccgcgcc gccgcgctct tcaaactgaa gattgaacag cagaacatca
     6301 gctggatcga gattgtcagc agccagttcg agaaaaatat cgtaggcctg ctcaagcgtt
     6361 tcatcttcag tcaggcgatt gtttagatcc atatccatag tcactacctg tttaacctct
     6421 gttggcgacg ttttacagca acgggctgaa gaagtaaaac agtcgctcgg cgacacgttg
     6481 ccataatgga cgttttagcc ataaacgggc atcgagcaga cgtgaacgcg aaatataatc
     6541 gtcctgaacg gcggcaaggt ctgcaccaaa acctttatcg tcgattgcca gggtaatctc
     6601 gaaattcagc cacagactac gcatatcaag gttaacggtg ccaaccagac ttagttcgcc
     6661 atcgaccagc acgctcttgg tatgcagtaa cccgccttca aactgataaa ttttaacccc
     6721 agcagcaagc agttccgtaa agaatgcgcg actggcccag ccgaccagca tcgagtcatt
     6781 ttttcgcgga aggataatac tgacatccac cccgcgctgc gccgccgtgc aaatcgcatg
     6841 aagtaaatca tcgcttggca caaagtaggg cgtggtcatg atcaaatatt cacgcgccga
     6901 ataagccgca gtcaataatg cctggtgaat gagatcttcc ggaaagccgg ggccagaagc
     6961 aattgtgtga atggtatgac cgctggcctg ctcaaacggc ataatattga catcaggtgg
     7021 tggcggtaga atacgtttcc ctgtttcaat ctcccagtcg caggaataaa taatccccat
     7081 cgcggtggcg atggggcctt ccatacgcgc catcagatca atccattgcc ctacgcccgc
     7141 atcttgtttg aagtagcgag ggtcgaccat attcatgctg ccggtgtacg cgatgtaatt
     7201 atcgatcatg atcatcttgc gatgttggcg caggtccata cggcgtaaaa acacacgcat
     7261 cagattgacc tttaaggctt cgaccacttc aataccggca ttacgcatta gctcgggcca
     7321 cgggctgcgg aaaaaagcta cactcccggc ggagtcgagc atcaatcggc aatgaatacc
     7381 acgccgtgca gccgccatta atgattcagc cacctggtcc gccatgccgc cgggctgcca
     7441 gatataaaac accatctcaa tattatggcg cgcgagctgg atgtcgcgga ttaacgcctg
     7501 catcacatca tctgactcgg tcatcagttg tagctgattc cctttgaccc cagcgatccc
     7561 ctgacgacgc tcgcaaagct tgaataatgg cgcagcgaca ctgctatttt cttcggcgaa
     7621 gatatgctta caggctttaa ggtcgttaag ccattttgcg gtggaaggcc acatcgctct
     7681 ggcgcgctca gcgcggcgtt tgcctaaatg gagctcgcca acggcaagat aggcaataat
     7741 tccgactaac ggcagaatgt aaataatcaa cagccaggcc atcgcggagg gaactgcgcg
     7801 tcgtttcatt agaatgcgta aagttacgcc tgcaatgagc aaccagtatc ccagaatggc
     7861 caaccaactc accaacgtat aaacggttgt catagattag aaatcctttt gaaagcgcat
     7921 tgttatgagt ttacgcatat ggattcatct ggcaaataaa aacgcgggaa aagcactggt
     7981 cagcgtcggg ggcgggggta taatgaccat tctgttattg catagagtag ttaacatgaa
     8041 gcggagtaga acggaagtgg ggcgctggcg catgcagcgt caggctagcc gacgtaaatc
     8101 gcgttggctt gaggggcagt cgcgccgaaa tatgcgtatc cacagcatca ggaagtgcat
     8161 tctaaacaaa cagcgtaact cgttattgtt tgcgatctac aatatctaaa tgttctgggc
     8221 accattgcgg tgcccaataa gtttactttt ccgtctttaa ctctattttt gcaccgattc
     8281 tttactgtca gctttcgttt cttgttcatc gttatcgctg aaaatgccat gaccggagtt
     8341 caacaacatt gaaacgagca tatcattatt aatctcttca ccatttaaaa ctcgcgccag
     8401 aatttcgctg ccaaacagtt gcggtgagag aatgatatcc ggatgtacca tcttaatctt
     8461 attcaggttt ttgctatcac tgacggcgag aactgttttg acatcactgc tcatatcttt
     8521 tgccgagagt acaacaaacg cgttatctgc atcgttatca ctcagcgcca gaatggcccg
     8581 gcaacgatcg attcccgctt tctttaatac tgaactgtca ttactgtcac cggggataac
     8641 atcagcgtta tcgcctaagc gttgctcaag ttgcttgata tcatcttcag gcaagttgct
     8701 gataaccgtt acgttttgtc cgcgttgatt cagttgcagg atcgtattga tggcgagaat
     8761 cgaatgtccg caaacaataa aatgatcttt acgatgcatt gtatgattgt ttccttttac
     8821 aagtttgttg aatcccccgc ggataagcgg accaaaaatt gaggtcatag atgtggcaaa
     8881 aacggtaatg ccggaaataa tgaccgaaat agtgaacaat cgtgctgatt cagaaacagg
     8941 gacaatatct ccatagccga cggttgacat ggtttctatc gaaaaataga acgcggtcat
     9001 caaactttct atccgcggat taaaaccttc gcttaaataa agcgcaccgt aggttgagta
     9061 aaacagtaat gtcgtgaaac taataaatgc aaaaattgtc ccggctgcgg cgctactgtg
     9121 ggagaagtct ttgcgcagta tcagcaaaaa caccagcgta aaaatgcaaa atccaatact
     9181 aaatttcagc cagggataaa aatgcagggt gtaaattagc gctatcaaca acaaaatgat
     9241 actgattgcc catgcgagct ttgcccggaa cagtaagcca atggagttca ggacaagaaa
     9301 gacgccgagc ataaatagtg gggcgtttgc cagcaaggag agatccaact ctgaaaatgc
     9361 tttgatatgg aaaatatcaa gcaaattgac cgacatacca tagattgttt taaaaataag
     9421 caatccattt aaaaagacgg ccagcgcgag aatgtcgtgc cgtagggtca cccataaatt
     9481 tgttgctgtt tgtttgaatg tagcccagtg actcactgat acctctgtaa ccacaatttt
     9541 gtttaattat cttaacgtgc acattttctg aacatacatg cgacacgtga atgtgttaaa
     9601 caatagtaga tgactttatc cgcagtaatc aaaggttttg agtgagcaac tctgcgtcag
     9661 gaaagggcat ttttcctgaa catgctcaca tcatataaag aaatacagat aaagttatta
     9721 tctgcttgtg gtggtgaatg cactgaccgg caataaggaa aggccaaaca agaacacggt
     9781 tgcaaaaacc gtgcccttaa atattgaatc tctattcaga acactttctt aaatggtttc
     9841 actgaaacgt gttcatagac acctgccgct acgtacgggt cagcatcggc ccaggcctga
     9901 gctgcttcca gcgattcaaa ttcagcaata acggttgagc cagtaaatcc cgcagcccct
     9961 ggatcgttac tgtctaccgc tggcattgga ccagctgtca acaaacgacc ttcatcatgc
    10021 agtaactgta aacgtgctaa atgtgccgga cgaacggaaa ggcgtttttc gaggctatca
    10081 gctttgtctt gagcgtaaat aacatacaac acgggcacaa ctccttgttc gggaaagttg
    10141 taaagtacgt tatttgaaag ggcgaagatc tgcaacggaa agatgatgtc tttgttaaaa
    10201 ccatgcgtaa agtaaggtca attacgccaa aaatgacatt ttcactgatc ctgatcgtct
    10261 tgccttattg aatatgattg ctatttgcat ttaaaatcga gacctggttt ttctactgaa
    10321 atgattatga cttcaatgac ccttgattta cctcgccgct tcccctggcc gacgttactt
    10381 tcggtctgca ttcatggtgc tgttgtggcg ggtctgctct atacctcggt acatcaggtt
    10441 attgaactac ctgcgcctgc gcagccaatt tctgtcacga tggttgcgcc tgctgatctc
    10501 gaaccgccac aagccgttca gccgccaccg gagccggtgg tagagccaga accggaacct
    10561 gagccgatcc ccgaaccgcc aaaagaagca ccggtggtca ttgaaaagcc gaagccgaaa
    10621 cctaagccaa aaccgaagcc ggtgaaaaag gtacaggagc agccaaaacg cgatgtcaaa
    10681 cccgtagagt cgcgtccggc atcaccgttt gaaaatacgg caccggcacg cccgacatca
    10741 agtacagcaa cggctgcaac cagcaagccg gttaccagtg tggcttcagg accacgcgca
    10801 ttaagccgta atcagccgca gtatccggca cgggcacagg cattgcgcat tgaagggcag
    10861 gttaaagtta aatttgatgt cacgccggat ggtcgcgtgg ataacgtgca aatcctctca
    10921 gctaaacctg cgaacatgtt tgagcgtgag gtgaaaaatg cgatgcgcag atggcgttat
    10981 gagccgggta agccaggcag tgggattgtg gtgaatatcc tgtttaaaat taacggcacc
    11041 accgaaattc agtaagcaga aagtcaaaag cctccgaccg gaggcttttg attattactc
    11101 aacaggtaag gcgcgaggtt ttccttcagg atcaaccgcg acatacttaa ataatgcttc
    11161 tgtcgcttta tagcgttgcc cgattggttc agacgctact tttttcaccc acacttcaat
    11221 attaatgctg accgatgtcg tccctttctg gacacagcgt gcatagcagc acaccacatc
    11281 gccgaccgca accggccgta agaaagtcat tccttcaacc cgcacggtca ctacgcgacc
    11341 gtgggcaatt tctttcgcca gaatagcgcc gccaatatcc atttgtgaca ttaaccaacc
    11401 accaaagatg tcaccattgg cattggtatc ggcgggcatg gctaaagtac gtaaaacaag
    11461 atcgccctga gggacgttat gtgttgtaga catgataaaa ccgacttaaa aggaaaatca
    11521 caggcgcgat gctactatga ttttcctgag gagaacagag gagatacagc gccagccccg
    11581 aaggactggc agtctggtta ggatttatct tcctgcggca tgtggcggta gatatagata
    11641 ccgcttaaca atgtgaagat taaggtaagg gcggtcaggc caaagacttt aaagttgacc
    11701 caaatatttt gcggcagcca gaatgcgatg tagatgtttg ccagaccgca aaggataaag
    11761 aaaacagccc aggccagatt cagcttcgac cataccggtt gcggtagcgt gagttcttta
    11821 cccagcatcc gctgaattag cggctttttc atcacccatt ggctgactaa cagggctccc
    11881 gcaaacagag cataaatgac tgtaaccttc catttaataa actcatcatt gtggaagaac
    11941 agcgtcaagc caccgaagac gaccaccaga acaaaagtga tcagggccat cttctcaacc
    12001 ttacgaaagc gaacccagct atagataagc acaatcgccg tggcgacgat cagcgccgca
    12061 gtagccgcat agatgtcata aatcttgtaa aacgcgaaaa agacaaccag cggtaaaaaa
    12121 tcaagaaact gcttcatttt acgattccgt aatcaaatgc ttaaggttat tggcgaatca
    12181 acatatacag acggaacaga tagatgagca atacggctga aatcaggttg ctcaaggtgt
    12241 tcgccagtac ggcaccaatt tccggggtta atgcggcaaa agaagaggca aaaagcagca
    12301 acagtgtttt tgccagcaac cagctcagta ctgcgggtgc caccagacgc atattcgccc
    12361 aagtcagccg catactgcta cgcatcgagg caaaaatgcc cattttgtcc tgtaccagca
    12421 tcaccggtgc cagggccagt aaaatggcca tgataattcc cggaacgacc accagcatga
    12481 tgccaatctg tactaaaagg gtagtcagaa aaatcagaat aaataacttg ggcaatatcg
    12541 gcgcactggc accaatagct cgcagcgcac tgactctctg acccgcagac accagctgga
    12601 taattaatat tacgcctccg gcgagaatgg cgttaccgat taatcctgaa aaagtggacg
    12661 ccgctgaagc ctgcagcaaa atttgttgct gttccggtga catattctga accaggtcga
    12721 acaacccact actgccgcta acgggcacgc cgtcattgag ctgcgcaagc tgtgcatcac
    12781 tgggtgagaa aacatgccct aacaccactg tgataaacgc acatagcaac gataccaaca
    12841 gaatggtcat aaattgatta cggaagaaat ttccggtgtc acggtatacg gactgcgccg
    12901 tgatagacat gcactctcct tgagttttat gcaggtgtta attagcgggc aattgtaccc
    12961 tggttaagcc tcgggtggca gcatcaggtc ttgtatggaa aagcatatct ttgtaaagcg
    13021 gaggtaattg ctggccttca ggcgtgggca aaccgcggca agtggcgaac gagttaatat
    13081 ttgcgtagcg aaaatattta aaaattgatt taaatcacgt taaccaggat tctcaatgca
    13141 acttctaaa
//
LOCUS       DAZUCE010000135        13031 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZUCE010000135 DAZUCE010000000
VERSION     DAZUCE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.135"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1183
                     /gene="proV"
                     /locus_tag="ACHY03_003311"
     CDS             <1..1183
                     /gene="proV"
                     /locus_tag="ACHY03_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFS3906503.1"
                     /translation="KNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGVKDASLAI
                     EEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIA
                     MVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSG
                     GMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDL
                     DEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRT
                     PNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDA
                     ALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNN
                     G"
     gene            1176..2240
                     /gene="proW"
                     /locus_tag="ACHY03_003312"
     CDS             1176..2240
                     /gene="proW"
                     /locus_tag="ACHY03_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFS3906504.1"
                     /translation="MADQNNPWDTTPAAESAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2297..3289
                     /gene="proX"
                     /locus_tag="ACHY03_003313"
     CDS             2297..3289
                     /gene="proX"
                     /locus_tag="ACHY03_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFS3906505.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3481..4665
                     /locus_tag="ACHY03_003314"
     CDS             3481..4665
                     /locus_tag="ACHY03_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3906506.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAACGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYNDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4789..5526
                     /gene="ygaZ"
                     /locus_tag="ACHY03_003315"
     CDS             4789..5526
                     /gene="ygaZ"
                     /locus_tag="ACHY03_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFS3906507.1"
                     /translation="MESPTTQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5516..5851
                     /gene="ygaH"
                     /locus_tag="ACHY03_003316"
     CDS             5516..5851
                     /gene="ygaH"
                     /locus_tag="ACHY03_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFS3906508.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            5942..6472
                     /gene="emrR"
                     /locus_tag="ACHY03_003317"
     CDS             5942..6472
                     /gene="emrR"
                     /locus_tag="ACHY03_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFS3906509.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6599..7771
                     /gene="emrA"
                     /locus_tag="ACHY03_003318"
     CDS             6599..7771
                     /gene="emrA"
                     /locus_tag="ACHY03_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFS3906510.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7788..9326
                     /gene="emrB"
                     /locus_tag="ACHY03_003319"
     CDS             7788..9326
                     /gene="emrB"
                     /locus_tag="ACHY03_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFS3906511.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9390..9905)
                     /gene="luxS"
                     /locus_tag="ACHY03_003320"
     CDS             complement(9390..9905)
                     /gene="luxS"
                     /locus_tag="ACHY03_003320"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFS3906512.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10055..11611)
                     /gene="gshA"
                     /locus_tag="ACHY03_003321"
     CDS             complement(10055..11611)
                     /gene="gshA"
                     /locus_tag="ACHY03_003321"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFS3906513.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11684..12112)
                     /locus_tag="ACHY03_003322"
     CDS             complement(11684..12112)
                     /locus_tag="ACHY03_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFS3906514.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12109..12675)
                     /gene="yqaB"
                     /locus_tag="ACHY03_003323"
     CDS             complement(12109..12675)
                     /gene="yqaB"
                     /locus_tag="ACHY03_003323"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFS3906515.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         3003 a         3450 c         3359 g         3219 t
ORIGIN      
        1 taaaaatctt tataaaatat ttggtgagca tccacagcga gctttcaaat atatcgaaca
       61 aggactttca aaagaacaaa ttctggaaaa aactgggcta tcgcttggcg taaaagacgc
      121 cagtctggcc attgaagaag gcgagatatt tgtcatcatg ggattatccg gttcgggtaa
      181 atccacaatg gtacgccttc tcaatcgcct gattgaaccc acccgcgggc aagtgctgat
      241 tgatggtgtg gatattgcca aaatatccga cgccgaactc cgtgaggtgc gcagaaaaaa
      301 gattgcgatg gtcttccagt cctttgcctt aatgccgcat atgaccgtgc tggacaatac
      361 tgcgttcggt atggaactgg ccggaattaa tgccgaagaa cgccgggaaa aagcccttga
      421 tgcactgcgt caggtcgggc tggaaaatta tgcccacagc tacccggatg aactctctgg
      481 cgggatgcgt caacgtgtgg gattagcccg cgcgttagcg attaatccgg atatattatt
      541 aatggacgaa gccttctcgg cactcgatcc attaattcgc accgagatgc aggatgagct
      601 ggtaaaatta caggcgaaac atcagcgcac cattgtcttt atttcccacg atctcgatga
      661 agccatgcgt attggcgacc gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg
      721 cacaccggat gaaattctca ataatccggc gaatgattat gtccgtacct tcttccgtgg
      781 cgttgatatt agtcaggtat tcagtgcgaa agatattgcc cgccggacac cgaatggctt
      841 aattcgtaaa acccctggct tcggcccacg ttcggcactg aaattattgc aggatgaaga
      901 tcgcgaatat ggctacgtta tcgaacgcgg taataagttt gtcggcgctg tctccatcga
      961 ttcgcttaaa accgcgttaa cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc
     1021 gttagcagtc gatgcacaaa cgcctcttag cgagttgctc tctcatgtcg gacaggcacc
     1081 ctgtgcggtg cccgtggtcg acgaggacca acagtatgtc ggcatcattt cgaaaggaat
     1141 gctgctgcgc gctttagatc gtgagggggt aaataatggc tgatcaaaat aatccgtggg
     1201 ataccacgcc agcggcggag agtgccgcgc aatccgcaga cgcctggggt acaccgacga
     1261 ctgcaccgac tgacggcggt ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg
     1321 agcattttaa tattctcgat ccgttccata aaacgctgat cccgctcgac agttgggtca
     1381 ctgaagggat cgactgggtc gttacccatt tccgtcccgt cttccagggc gtgcgcgttc
     1441 cggttgatta tatcctcaac ggtttccagc aattgctgct gggtatgccc gcgccggtgg
     1501 cgattatcgt tttcgctctc atcgcctggc agatttccgg ggtcggaatg ggcgtggcga
     1561 cgctggtttc gctgattgcc atcggcgcaa tcggtgcctg gtcgcaggca atggtgactc
     1621 tggcgctggt gttaaccgcc ctgctgttct gtatcgtcat cggtttgccg ttggggatct
     1681 ggctggcgag aagtccgcga gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga
     1741 ccacgccagc gtttgtttat ctggtgccaa tcgtcatgct atttggtatc ggtaacgtgc
     1801 cgggcgtggt ggtgacgatc atctttgctc tgccgccgat tatccgtctg accattctgg
     1861 ggattaacca ggttccggcg gatctgattg aagcctcgcg ctcattcggt gccagcccgc
     1921 gccagatgct gttcaaagtt cagttaccgc tggcgatgcc gaccattatg gcgggcgtta
     1981 accagacgct gatgctggcc ctttctatgg tggtcatcgc ctcgatgatt gccgtcggcg
     2041 ggctgggtca gatggtactt cgcggtatcg gtcgtctgga tatggggctt gccaccgttg
     2101 gcggcgtcgg gattgtgatc ctcgccatta tcctcgatcg tctgacgcag gccgttgggc
     2161 gcgactcacg cagtcgcggc aaccgtcgct ggtacaccac tggccctgtc ggtctgctga
     2221 cccgcccatt cattaagtaa ctctgcactt gcccggtgac gccgggcata tcaccctgcc
     2281 aaaaaaagga ataacaatgc gacatagcgt actttttgcg acagcgtttg ccacgcttat
     2341 ctctacacaa acttttgctg ccgatctgcc gggcaaaggc attactgtta atccagttca
     2401 aagcaccatc accgaagaaa ccttccagac gctgctggtc agccgtgcgc tggagaaatt
     2461 aggctatacc gtcaacaaac ccagcgaagt ggattacaac gttggctaca cctcgcttgc
     2521 ttccggcgat gcaaccttca ccgccgtgaa ctggacgcca ctgcatgaca acatgtacga
     2581 agctgccggt ggcgataaga aattttatcg tgaaggggta tttgttaacg gcgcggcaca
     2641 gggttacctg atcgataaga aaaccgccga ccagtacaaa atcaccaaca tcgcacaact
     2701 gaaagatccg aagatcgcca aactgttcga taccaacggc gacggtaaag cggatttaac
     2761 cggttgtaac ccaggctggg gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga
     2821 actgaccaac accgtgacgc ataatcaggg gaactacgca gcgatgatgg ccgacaccat
     2881 cagtcgctac aaagagggca aaccggtgtt ttactacacc tggacgccgt actgggtgag
     2941 taatgagctg aagccaggga aagatgtggt ctggttgcag gtgccgttct ctgcactgcc
     3001 gggcgataaa aatgccgata ccaaactgcc gaatggcgcg aattacggct tcccggtcag
     3061 caccatgcat atcgttgcca ataaagcctg ggccgagaaa aacccggcag cagcgaaact
     3121 gtttgccatt atgcagttgc cagtggcaga tattaacgcc cagaacgcca ttatgcatga
     3181 cggcaaagcc tcagaaggcg atattcaggg ccacgttgat ggctggatca aagcccacca
     3241 gcaacagttc gatggctggg tgaatgaggc gttggcagcg cagaagtaat ttttattcgg
     3301 gcggataagg cgtttacgcc gcatccgccg ttctgtacac aatgcctgat gcgacgctgg
     3361 cgcgtcttat catgcctaca aaccacaccg cacatttcaa caatctatct ttcatcccat
     3421 attcatcaac atccgctatt attgatttcc agcttaatca tcacctgatg aacaaaaata
     3481 atgaccaaac ctaatcatga gcttagcccg gcgctaatcg tgctgatgtc tatcgccacc
     3541 ggtctggcgg tagccagtaa ctattacgcc cagccattgc tcgacaccat cgcgcgtaac
     3601 ttttcccttt ccgccagttc ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc
     3661 gcaggtctac tgtttcttgt tcccctcggt gatatgtttg aacgccgccg cctgattgtc
     3721 tcgatgacct tactggcggc atgcggcatg ttgattaccg ccagcagtca gtcgctggcg
     3781 atgatgatcc tcggtacggc attaaccggt ttattctcag tcgtggcaca aattctggtt
     3841 ccactggcag cgacgctggc ttcaccggat aaacgcggca aagtggttgg caccattatg
     3901 agcggcctgc tgttggggat attgctggca cgaaccgttg ccgggttact ggcaaacctc
     3961 ggcggctggc gcaccgtctt ttgggttgcc tcggtgttaa tggctctgat ggcgctagca
     4021 ttatggcgtg gtctgccaca aatgaaatca gaaacccacc tcaattaccc acagttgttg
     4081 ggttccgttt tcagtatgtt tatcagcaat aagatcctgc gcacccgcgc gttgctgggc
     4141 tgcctgacct ttgccaactt cagcattctc tggacctcaa tggccttttt gcttgccgca
     4201 ccgcctttta attacaacga tggcgtcatt ggtctgtttg gacttgcggg ggctgccgga
     4261 gcgttgggcg ctcgtccggc gggcggtttt gccgacaagg gcaaatcgca ccacaccaca
     4321 actttcggtc tgctgctgct gttactttca tggctggcaa tctggtttgg tcacacttcc
     4381 gtactggcgt tgattatcgg cattctggtg ctggacctca ccgtacaggg cgtgcatatc
     4441 actaaccaga cggtaattta tcgaatacat cctgatgcgc gtaatcgcct gaccgcaggt
     4501 tacatgacca gctactttat tggcggtgcc gccggttcgc taatttcagc ctcagcctgg
     4561 caacatggcg gttgggctgg cgtttgtctg gctggcgcga cgattgccct ggttaactta
     4621 ctggtctggt ggcgaggttt tcatcgtcag gaagccgcaa attaagcaat cagcaaccgt
     4681 tttcgtggct ttacacttat aagggtgtta agaagcccat cagtctgata aggttaagat
     4741 attcattcag tctatttata atattaacaa tcgttaagcg tacactctat ggaaagccct
     4801 actacacagc ctgctcctgg ttcggcgacc ttcatggaag gatgcaaaga cagtttaccg
     4861 attgttatta gttatattcc ggtggccttt gcgttcggtc tgaatgcgac ccgtctggga
     4921 ttctctcctc tcgaaagcgt ttttttctcc tgcatcattt atgcaggcgc gagccagttc
     4981 gtcattaccg cgatgctggc agccgggagt agtttgtggg ttgctgcact gaccgtcatg
     5041 gcaatggatg ttcgccatgt gttgtatggc ccgtcactgc gtagccgtat tattcagcgt
     5101 ctgcaaaaat cgaaaaccgc cctatgggcg tttggcctga cggatgaggt ttttgccgcc
     5161 gccaccgcaa aactggtacg caataatcgc cgctggagcg agaactggat gatcggcatt
     5221 gccttcagtt catggtcatc gtgggtattt ggtacggtaa taggggcatt ctccggcagc
     5281 ggcttgctgc aaggttatcc cgccgttgaa gctgcattag gttttatgct tccggcactc
     5341 tttatgagtt tcctgctcgc ctctttccag cgcaaacaat ctctttgcgt taccgcagcg
     5401 ttagttggtg cccttgcagg cgtaacgcta ttttctattc ccgtcgccat tctggcaggc
     5461 attgtctgtg gctgcctcac tgcgttaata caggcattct ggcaaggagc gcccgatgag
     5521 ctatgaggtt ctgctgcttg ggttactggt tggcgcggcg aattattgct tccgctattt
     5581 gccgctgcgc ctgcgtgtgg gtaatgcccg cccaaccaaa cgtggcgcgg taggtatttt
     5641 gctcgacacc attggcatcg cctcgatatg cgctctgctg gttgtctcta ccgcaccaga
     5701 agtgatgcac gatacacgcc gtttcgtgcc cacgctggtc ggcttcgcgg tactgggtgc
     5761 cagtttctat aaaacacgca gcattatcat cccaacactg cttagtgcgc tggcctatgg
     5821 gctcgcctgg aaagtgatgg cgattatata acccacaaga atcatttttc taaaacaata
     5881 catttacttt atttgtcact gtcgttacta tatcggctga aattaatgag gtcataccca
     5941 aatggatagt tcgtttacgc ccattgaaca aatgctaaaa tttcgcgcca gccgccacga
     6001 agattttcct tatcaggaga tccttctgac tcgtctttgc atgcacatgc aaagcaagct
     6061 gctggagaac cgcaataaaa tgctgaaggc tcaggggatt aacgagacgt tgtttatggc
     6121 gttgattacg ctggagtctc aggaaaacca cagtattcag ccttctgaat taagttgtgc
     6181 tcttggatca tcccgtacca acgcgacgcg tattgccgat gaactggaaa aacgcggttg
     6241 gatcgaacgt cgtgaaagcg ataacgatcg ccgctgcctg catctgcaat taacggaaaa
     6301 aggtcacgag tttttgcgcg aggttttacc accgcagcat aactgcctgc atcaactctg
     6361 gtccgcgctc agcacaacag aaaaagatca gctcgagcaa atcacccgca aattgctctc
     6421 ccgtctcgac cagatggaac aagacggtgt ggttctcgaa gcgatgagct aacgcgtcat
     6481 ctcgctcaaa aatccagatt tataaaagaa aaaatgactg gccagcatcg caacatgctg
     6541 gcctttttgg caagcaggtc ggctcagccg atgagttaag aagatcgtgg agaacaatat
     6601 gagcgcaaat gcggagactc aaaccccgca gcaaccggta aagaagagcg gcaaacgtaa
     6661 gcgtctgctc ctccttctca ccttgctctt tataattatt gccgtagcga tagggattta
     6721 ttggtttttg gtactgcgtc acttcgaaga aaccgatgac gcatacgtgg cagggaatca
     6781 aattcaaatt atgtctcagg tgtctggcag cgtgacgaaa gtctgggccg ataacaccga
     6841 ttttgtaaaa gaaggcgacg tgctggtcac tctcgacccg acagatgctc gccaggcgtt
     6901 tgaaaaagcc aaaactgcac tggcttccag cgttcgccaa acccaccagc tgatgattaa
     6961 cagcaaacag ttgcaggcga atattgaggt gcaaaaaatc gccctcgcga aagcacaaag
     7021 cgactacaac cgccgtgtgc cgctgggcaa tgccaacctg attggtcgcg aagagctgca
     7081 acacgcccgc gacgccgtta ccagtgccca ggcgcaactg gacgtcgcga ttcaacaata
     7141 caatgccaat caggcgatga ttctggggac taaactggaa gatcagcctg ccgtgcaaca
     7201 ggctgccacc gaagtacgta acgcctggct ggcgctggag cgtactcgta ttgtcagtcc
     7261 gatgaccggt tatgtctccc gccgcgcggt acagcctggg gcgcaaatta gcccaacgac
     7321 gccgctgatg gcggtcgttc cagccaccaa tatgtgggtg gatgccaact ttaaagagac
     7381 gcagattgcc aatatgcgta tcggtcagcc ggtcactatc accaccgata tttacggcga
     7441 tgatgtgaaa tacaccggta aagtggttgg tctggatatg ggcacaggta gcgcgttctc
     7501 actgcttcca gcgcaaaatg cgaccggtaa ctggatcaaa gtcgttcagc gtctgcctgt
     7561 gcgtatcgaa ctggaccaga aacagctgga gcaatatccg ctgcgtatcg gtttgtccac
     7621 gctggtgagc gtcaatacca ctaaccgtga cggtcaggta ctggcaaata aagtacgttc
     7681 cactccggta gcggtaagca ccgcgcgtga aatcagcctg gcacctgtca ataaactgat
     7741 cgacgatatc gtaaaagcta acgctggcta atccagaggt gcgtgtgatg caacagcaaa
     7801 aaccgctgga aggcgcgcaa ctggtcatta tgacgattgc gctgtcactg gcgacattca
     7861 tgcaggtgct ggactccacc attgctaacg tggcgatccc cactatcgcc gggaatctgg
     7921 gctcatcgct cagccaggga acgtgggtaa tcacttcttt cggggtggcg aatgccatct
     7981 cgatcccgct taccggctgg ctggcaaagc gcgtcgggga agtgaaactg ttcctctggt
     8041 caaccatcgc ctttgctatt gcgtcgtggg cgtgtggtgt ctccagcagc ctgaatatgc
     8101 tgatcttctt ccgcgtgatt caggggattg tcgccgggcc gttgatcccg ctttcgcaaa
     8161 gtctattgct gaataattac ccgccagcca aacgctcaat cgcgctggcg ttgtggtcga
     8221 tgacggtgat tgtcgcgcca atttgcggcc caatcctcgg cgggtatatc agcgataatt
     8281 accactgggg ctggatattc ttcatcaacg tgccgattgg cgtggcggtg gtgttgatga
     8341 ccctgcaaac cctgcgcgga cgtgaaaccc gcaccgaacg gcggcggatt gatgccgtgg
     8401 ggctggcact gctggttatt ggtatcggca gcctgcagat tatgctcgac cgcggtaaag
     8461 agctggactg gttttcatca caggaaatta tcatccttac cgtggtggcg gtggtggcta
     8521 tctgcttcct gattgtctgg gagctgaccg acgataaccc gatagtcgat ctgtcgttgt
     8581 ttaagtcgcg caacttcacc atcggctgtt tgtgtatcag cctcgcgtat atgctctact
     8641 tcggcgcgat tgttctgctg ccgcagttgt tgcaggaggt ctacggttac acggcgacct
     8701 gggcaggttt ggcctctgcg ccggtaggga ttattccagt gatactgtcg ccgattatcg
     8761 gccgcttcgc gcataaactg gatatgcggc ggctggtaac cttcagcttt attatgtatg
     8821 ccgtctgctt ctactggcgt gcctatacct ttgaaccggg catggatttt ggcgcgtcgg
     8881 cctggccgca gtttatccag gggtttgcgg tggcctgctt ctttatgccg ctgaccacca
     8941 ttacgctgtc tggtttgcca ccggaacgac tggcggcggc atcgagcctc tccaacttta
     9001 cgcgtacgct ggcggggtct atcggcacgt cgataaccac gaccatgtgg accaaccgcg
     9061 agtcgatgca ccatgcgcag ttaaccgagt cggtaaatcc gttcaacccg aatgcccagg
     9121 cgatgtacag tcaactggaa gggcttggga tgacgcaaca gcaggcctcc ggctggattg
     9181 cccagcagat cactaaccag gggctgatta tttccgccaa tgagatcttc tggatgtcag
     9241 ccgggatatt cctcgtcttg ctggggctgg tgtggtttgc taaaccgcca tttggcgcag
     9301 gtggcggcgg aggcggtgcg cactaagtac aactaagcca gttcatttga actggctttt
     9361 ttcaattaat tgtgaagata gtttactgac taaatgtgca gttcctgcaa cttctctttc
     9421 ggcagcgcca gttcttcgtt gctgttgatg cgcacgtcac gttccagaat gctacgcgca
     9481 atatcctgtg cttcctgcaa cgagtgcatc tggtaagtgc cacactggta gacgttcagt
     9541 tccgggatct gattctgatc ctgcactttc agcacgtctt ccattgccgc tttccaggca
     9601 tcagcaacac gctgctcatc tggcgtacca atcagactca tataaaaacc ggtgcggcag
     9661 cccattggcg aaatatcgat aatctctaca ccattaccgt taagatggtt acgcataaaa
     9721 ccagcaaaca ggtgctccag ggtatggatc cctctttctg gcatcacttc tttgttcggc
     9781 acgcagaagc gcagatcgaa cacggtgatt gcgtcgccat gcggggtgtt cattgttttc
     9841 gccacccgaa ctgcaggcgc ttccatccgg gtatgatcga ctgtgaagct atctaacaac
     9901 ggcatttagc cacctccggt aattttttta aaaattttct gaactctttc ttcccaggcg
     9961 agtctgagta tatgaaagac gcgcatttgt tatcatcatc cctgaattca gagatgaaat
    10021 tttggccact cacgagtggc ctttttcttt tctgtcaggc gtgtttttcc agccacaccg
    10081 caaacggttc ggtatctgcg gcttccattt cctgctgacg gcgttcagat gcctcgcgct
    10141 cggctacaaa atcctcttcg cgcagaattt ccagcggctc ttcacgcagc agattacggt
    10201 acgcttcagc aaacgctttg cctgttccgc caataccagt atcaatcata gaccttaaga
    10261 tacgggcaga gaaagtcaga tcgggattat cgaagcaggc gaccagttca tcacacactt
    10321 tctgatacgc ttcgccgccg ttaatgctat ccagcgtttg cgcgacgcgt ttcagatcgc
    10381 ggaacagatc tttacccacc tgtggtaacg ggaactgtgc ggtttcgcag ccgataccca
    10441 gcgtcagacc cggtttgcga ccttcgagga tcacccggtt ccagttaacg cgcgtacagg
    10501 caagttcgct actgctcatt tccggtgcat cagccagcgc acaccagacc ataaacaggt
    10561 cgaggaatcg cacctgctgt tcatctacac caatcggcga gaacgggttg atgtccagcg
    10621 aacgcacttc aatatattca atgccgccac gtaacagcgc atcagaaggc gactcgccgc
    10681 tgcgggtaac gcgttttgga cgaatcggcg cgtacagttc gttttcaatc tgcagcacgt
    10741 tgctgttgat ttgcagcctc ttaccgtctt tctcaatacc aatcttcgcg tactcttccg
    10801 atggcgtttt gattgcctgt ttaaggcccg ctacgtactc gtaaagatcg ttgaaggtaa
    10861 taccaagatt gctttgcgat ttattggtat agccgaggtc gctcaaacga agagaggtcg
    10921 catacggcag gtaatacata ccgcactcgg ttttctcaaa cggcagcgac gttggttttc
    10981 cttgcaggaa agaagaacaa atcgccggag atgcaccaaa cagataagga atgacccaac
    11041 cgaaacgata gtaattgcgg ataacgcgga aatagcccgc agaaattttc tctttggcat
    11101 cagcgcccga gatatcaccg cacttcgctt gccagaatgc cattggcaaa gagaaattgt
    11161 aatgcacgcc ggaaatggtt tgcatcagcg cgccatagcg atttttcagc ccttcacgat
    11221 acagcgtttt aaagcgtccg gtgttagaag tgccgtactg tgccagttcg atgtcctgac
    11281 cttctgcgat gtagcatggc atacttaacg gccacatccg ctcatcgccc atattgcgcg
    11341 ccgtataacg atgcagatcg cgcataaagg tcagcatatg ttcaatatca ccatccactg
    11401 gtgtaataaa ttccagcaat gcttccgcaa aatcggtagt aatccattta tgcgtcagtg
    11461 cggaacctaa tgcttcagga tgacctgttg ttgccagtgt gccatcagca ttaacacgca
    11521 aagtttcgcg ctccagccca cgctgtatcc cctttaacgc ctgaggatgt ttttccagcc
    11581 aggccagcgc ctgtgatacg tccgggatca aattgacctc ccgcctgtca aaatcgtttt
    11641 aattagcata actgtaatgg tgaccatatg tgcaggccta caattagtgc caccacatca
    11701 tgccctgaac ggtagctgct gcaactgcaa catagcgtaa cgctttacca aggcacaaaa
    11761 aaaagattac cggtccccac gagatgcgca tccatcccgc taacagacac agtaaatcgc
    11821 caaccacggg catccagctt aataatagcg tgactgcacc atagcgtttc agccagccgg
    11881 tagctttctc ttgccagcgc gatgttttac gcaatggaaa gaaacgccca aggataacgt
    11941 tagttaaccc tccaaggcta ttacccattg ttgctgttaa aactaaaacc cagggatgac
    12001 tgatcccgga aagcaacatt gccaccagca cgacttcgga gttgccgggt aatagtgtag
    12061 cgctgagaaa actactggca aacaacgaga aaagcgataa cgcttcactc acagcaagcg
    12121 aacatccaca gcgtccatgc ctgctgcacg ggccgcctga ataccgaaat cggcatcttc
    12181 aaagaccaca cactgcgtcg gttgcacgcc catacgctgc gcgcacaaca aaaatgtgtc
    12241 tggcgcgggt ttatggtgtt tgacgtgatc ggcagcgacg acggcgtcaa aataacggcg
    12301 taatcccagg tgcgccagca atgcctcagc gatggcgctt tcactccccg ttcctacagc
    12361 cattgggcga cgaccatgcc aacttttcac cacttcaaca agaggaagcg gctcgacact
    12421 atccagcagc atacttctta ctgcttctgt tttttcacgc gctaacgcat gcgggtcgag
    12481 atcggcctga ttcagctcaa taattgcctg agcaatacgc caggtgggcg atccattaag
    12541 cgcaatcatc gcctgaacat cgtactgaag accgtagtgc cctaatactt cgcgccacgc
    12601 tttacggtgc gtaggctccg tatccaggat tgtgccatcc atatcaaaaa ttaaacctgc
    12661 ataacgctcg tacatggtct tctcgcaagt cgaaaaatat gacgctactt tagcgtaatt
    12721 gcttgatttt gtcgctgatg gcgaaggaga agaaaattca ggaaatggat aaagtagtta
    12781 tttagaaatg agatattttt gaaggaaatt ttttggagaa gatggtgcat ccgggaggat
    12841 gactcactgg cgttcgccct tcgggccgtt gctaaagcaa cgttatcctt cctggtgcct
    12901 gcggttaatt ccgctacctc ttcgatatat tcattgctga agatttcgga gaatatggtg
    12961 catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg
    13021 agctacggat g
//
LOCUS       DAZUCE010000136        13010 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZUCE010000136 DAZUCE010000000
VERSION     DAZUCE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.136"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..234)
                     /locus_tag="ACHY03_003324"
     CDS             complement(<1..234)
                     /locus_tag="ACHY03_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS3906516.1"
                     /translation="MRYNGLNNMFFPLCLINDNHSVTSPSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKMDDHEKVIGLIQKMKRIYDSLPSG"
     gene            complement(412..2061)
                     /gene="actP"
                     /locus_tag="ACHY03_003325"
     CDS             complement(412..2061)
                     /gene="actP"
                     /locus_tag="ACHY03_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFS3906517.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(2058..2372)
                     /gene="yjcH"
                     /locus_tag="ACHY03_003326"
     CDS             complement(2058..2372)
                     /gene="yjcH"
                     /locus_tag="ACHY03_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFS3906518.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2573..4531)
                     /gene="acs"
                     /locus_tag="ACHY03_003327"
     CDS             complement(2573..4531)
                     /gene="acs"
                     /locus_tag="ACHY03_003327"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFS3906519.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4924..6360
                     /gene="nrfA"
                     /locus_tag="ACHY03_003328"
     CDS             4924..6360
                     /gene="nrfA"
                     /locus_tag="ACHY03_003328"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFS3906520.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6405..6971
                     /gene="nrfB"
                     /locus_tag="ACHY03_003329"
     CDS             6405..6971
                     /gene="nrfB"
                     /locus_tag="ACHY03_003329"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFS3906521.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6968..7639
                     /gene="nrfC"
                     /locus_tag="ACHY03_003330"
     CDS             6968..7639
                     /gene="nrfC"
                     /locus_tag="ACHY03_003330"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFS3906522.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7636..8592
                     /gene="nrfD"
                     /locus_tag="ACHY03_003331"
     CDS             7636..8592
                     /gene="nrfD"
                     /locus_tag="ACHY03_003331"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFS3906523.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8612..10330
                     /gene="nrfE"
                     /locus_tag="ACHY03_003332"
     CDS             8612..10330
                     /gene="nrfE"
                     /locus_tag="ACHY03_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFS3906524.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVVLASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVMLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIDELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10323..10706
                     /gene="nrfF"
                     /locus_tag="ACHY03_003333"
     CDS             10323..10706
                     /gene="nrfF"
                     /locus_tag="ACHY03_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFS3906525.1"
                     /translation="MSKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10703..11299
                     /gene="nrfG"
                     /locus_tag="ACHY03_003334"
     CDS             10703..11299
                     /gene="nrfG"
                     /locus_tag="ACHY03_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFS3906526.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11641..12954
                     /gene="gltP"
                     /locus_tag="ACHY03_003335"
     CDS             11641..12954
                     /gene="gltP"
                     /locus_tag="ACHY03_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFS3906527.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
BASE COUNT         2916 a         3347 c         3607 g         3140 t
ORIGIN      
        1 tcctgatggt aaactatcat aaattctttt cattttctga atcaagccta tcactttttc
       61 atgatcatcc attttgaaaa gagagagggc tttattgtca attaacgtgt ttttatgatg
      121 tttgctgtaa ttatctgatt ttgttttctt tgtatgtgat ggacttgtga cagagtggtt
      181 atcgttaatc aggcaaagag ggaaaaacat attatttaaa ccattatagc gcataaaata
      241 tccttataat taacaagaaa gaaaaggcat attctctgca ttatcatttt ctacgactgt
      301 caaaaatcgc tcatttttta atgagtttat ttgtttaata ttatgggaaa aggtgatgca
      361 tttgggagag gaagagtatt ccccggtcag acgaccgggg aaggggagag attaatgcgc
      421 gcggccttgc tcaacgccaa agccggtctg ggagcggata aactgcgcgc ggaacagttc
      481 acgctcacgc gcgccttccg ctgagttatc ggttgccgag aagaaccaga tgccgaggaa
      541 tgccacgctg atagagaaca gcgccgggta ttcatacggg aagatggctt tttcgtgacc
      601 gaggatctgt acccaaatcg tcgggccgag gatcatcagt accactgcgg taatcagccc
      661 cagccagcca cccatcatcg cgccacgcgt ggtcagtttc gaccagtaca tagaaagcag
      721 aatgatcggg aagttacagc tcgccgcgat ggcaaacgcc agccccacca taaaggcgat
      781 gttctgattc tcaaacagca cgccgaggat aatcgcaatc acgccgagga tcagtacggt
      841 gatttttgat acccgcagct cttcacgttc ggtcgcacct tttttgaaga cgttagcgta
      901 caggtcatgc gaaaccgcgg atgcgcctgc cagcgtcaga cccgcaacca ccgcgaggat
      961 agtggcgaaa gcaaccgctg aaataaaacc gaggaacagg ttgccgccca ctgcattcgc
     1021 caggtgaacg gccgccatgt tgttaccacc aatcagatgg cccgccgcgt ctttatattc
     1081 cggatttgca ccgaccagca tgatcgcgcc aaagccgata ataaaggtca ggatatagaa
     1141 gtagcccata aacccggtgg cgtagaacac gctcttacgt gcttcgcggg catcgctgac
     1201 cgtaaagaag cgcatcagaa tgtgcggcaa gccagccgta ccaaacatca atccaagccc
     1261 cagcgacagc gcggagatcg gatctttcac cagccctccc ggcttcatga tgtcgacacc
     1321 tttcgggtgt accgccatcg cttcgctgaa cagattgttg aagctaaagc cgacgtgttt
     1381 catcaccata aaggccataa agctggcacc gaacagcaac agcacagctt tgataatttg
     1441 tacccaggtg gtcgccagca tgccgccgaa caggacgtac atcatcatca gcacgccgac
     1501 cagcaccact gcaatgtgat agttaaggcc aaacagcagc tcgatcagtt tacctgcgcc
     1561 caccatctgg gcgataaggt aaagcgccac caccaccaga gaaccacagg ccgaaagaat
     1621 acgaatcggc ccttgtttca gacgataaga ggctacatcg gcaaaggtgt aacgccccag
     1681 gttacgcaga cgttcggcaa tcaggaacaa aatgatcggc cagcccacca ggaagcccag
     1741 cgagtaaatt aagccgtcat agccggaggt aaacaccagt gcggagatcc ccaaaaatga
     1801 ggcggcggac atatagtcac cggcaatcgc cagcccgttc tgaaaaccgg tgatattgcc
     1861 gcctgcggtg taatagtcat tacgcgaacg tacgcgtttt gacgcccagt aggtaatgcc
     1921 gagcgtaaac acgacgaaaa tcaggaacat aataatcgcc tgccagttcg ttggctggcg
     1981 ctctacggcc ccgctaatag catccgcggc gttagctgcg aaagggagtg tggcggcaag
     2041 cgccgtcaga actcttttca tgatgcttgt acctcatgca ggacttcatt attaagacgg
     2101 tcgaattcgc cgttcgcccg ccagatgtag ataccagtga gaacaaagga gatcactatt
     2161 accccaacac cgactggaat accgcgtgtg acgctggtgt tcggattcag cggggtgccc
     2221 agccagccgg gcgcgaaggc gatcagtaaa ataaagccga tataaactgc cagcataata
     2281 atcgacagga tggtggcaaa ccgttgccgt ttttcgacta actccctgaa atgcgcattg
     2341 tcttctatcc gctgataaat cgtaccattc atcacagatt ctccagaggt aatgtaggga
     2401 ttgttttaat tcccgctccc ttatgggaga aggttaacgc tcgggtaacc cttgccgaat
     2461 gtaggccgga taaggcgtta tacgccgcat ccggcaatca atgcctgatg cgacgctgtc
     2521 gcgtcttatc aggcctacaa accgttaccg actcgcatcc ggcaattgtg ggttacgatg
     2581 gcatcgcgat agcctgcttc tcttcaagca gcttctcgac tacgccagga tcggcaagcg
     2641 tcgaggtatc gcccaggttg ctggtatcgc ccgccgcaat tttgcgcaga atacggcgca
     2701 taattttgcc ggagcgggtt ttaggcaggg agtcggtcca gtgcagcacg tctggcgtcg
     2761 ccagcgggcc aatctcttta cgcacccagt tgcggacttc tgcgtacagt tctggtgacg
     2821 gttcctcccc gtgattaagc gtgacgtagg cgtagatcgc ctgaccttta atattgtgcg
     2881 gaataccgac gacagcggct tcggcgattt tcggatgcgc caccagcgcc gactcaatct
     2941 ccgccgttcc cagacgatga ccggagacgt tcagcacgtc gtccacgcgc ccggtgatcc
     3001 agtaatagcc atcttcatcg cgacgcgcgc cgtcgccgct gaaatacata tttttgaagg
     3061 tggagaagta ggtctgctca aaacgttcgt gatcgccaaa cagcgtacgc gcctgacccg
     3121 gccaggagtc ggtgatcacc agactgcctt cggtggctcc ttccagcggg ttaccttcgt
     3181 tatcgaccag cgccggttgc acgccgaaga acggacgtgt tgccgaaccg gctttcagct
     3241 cggtagcgcc aggcagcggg gtgatcatga aaccgccggt ttcggtctgc caccaggtat
     3301 cgaccaccgg acatttctcg ttgccgattt ttttccagta ccactcccac gcttccgggt
     3361 taattggctc gcccacggaa ccgagaatgc gcagcgacga acggtcggtg ccttcgatcg
     3421 ctttatcgcc ttccgccatc agcgcgcgaa tcgccgtggg cgcggtatag agaatattga
     3481 cctgatgctt gtccaccacc tgtgccatac gggcaggcgt cggccagttc ggtacgcctt
     3541 caaacatcag cgtggtcgcg ccgcaggcca gcgggccgta cagcaaataa ctgtgtccgg
     3601 tcacccagcc cacatcggcg gtgcaccagt agatatcgcc cggatgataa tcaaagacat
     3661 atttaaaggt cagcgccgcg tacaccagat aaccgccggt agtgtgcagt acgccttttg
     3721 gttttccggt agaaccggag gtatagagaa taaacagcgg atcttcggcg ttcatctctt
     3781 ccgcctggtg ctgatcgctg gcttgctcaa ccaggtcgtg ccaccacagg tcgcgccctt
     3841 cctgccagtc aattttcccg ccagtacgct tcagtaccac cacatgctct acgctggtga
     3901 cgttcgggtt ttttagtgcg tcatcaacgt ttttcttcag cggaatacta cgcccggcgc
     3961 gcacgccttc gtcggaagtg atcaccagtc gtgagttgga atcgataatg cgcccggcaa
     4021 ccgcttccgg cgagaagcca ccgaaaatta ccgaatgcac cgcgccaata cgggcgcagg
     4081 ccagcatcgc aaccgcggct tccggcacca tcggcatata aatcgccacc acatcacctt
     4141 ttttaatgcc cagctcgagc agggtattgg cgaagcggca gacgtcgcgg tgcagctctt
     4201 tatagctgat atgtttgctc tggctggcgt cgtcgccttc ccagatgatg gcggtacgat
     4261 cgccgttttc ttgcagatgg cggtcaaggc agtttgccgc cagattcagc gtgccgtcct
     4321 cgtaccattt aatggacaca ttaccggggg caaaggaggt gtttttcacc ttctggtacg
     4381 gtttgatcca gtcgagaatt tttccctgtt cgccccagaa ggtatcaggt gcgttaatag
     4441 attgttgata catcgcctcg tactgctgag ggtttatcag gcaacggtct gcgatgttgg
     4501 caggaatggt gtgtttgtga atttgactca tgcttttgtt ctccttgtag gatgttaata
     4561 atatgtggca taagcgttaa atgtaggggt attggcagtt ttgtttagta tttgggcgac
     4621 agatcacgca aaagtagaat tgtgcaaata aacggcaggg taatttttga aggtcagcaa
     4681 caaaagttga ttaattcttt gaggaacatg cagttatgca tgctgttgaa aaagaggaag
     4741 atactgacta actctaaagt ggtattttac atgcacttac aattgattaa agacaacatt
     4801 cacagtgtgg ttatttgtta cacatagggg cgagcaatgt catgacagtg taggtgcggt
     4861 tactgtcgtg aaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc
     4921 cctatgacaa ggataaaaat aaacgcacgc cgtatcttca gcttattgat tccttttttc
     4981 tttttcactt ctgttcacgc tgaacaaacg gctgctcccg caaaacctgt aactgtggaa
     5041 gcgaagaatg aaacctttgc cccgcagcat cccgatcaat atctctcctg gaaagccacc
     5101 tcggaacagt cagagcgtgt tgacgccctg gcggaagatc cacggctggt gatcctgtgg
     5161 gcggggtatc ccttctcgcg cgattacaac aagccgcgtg gacatgcctt tgctgtgacc
     5221 gatgtgcgtg aaaccctgcg taccggtgcg ccgaaaaacg ctgaagatgg tccgctaccg
     5281 atggcgtgct ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa agacggcgaa
     5341 gatggctact tccacggtaa atgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt
     5401 tgtgccgatt gccataacac cgcctctcca gagttcgcca aaggcaaacc ggagttaacc
     5461 ctttcccgtc cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc
     5521 ggacgtttcg accagcaatc gatggtttgc ggtcagtgcc atgtggagta ttacttcgac
     5581 ggcaaaaaca aagcggttaa attcccgtgg gatgacggca tgaaagtcga aaatatggag
     5641 cagtattacg acaaaattgc cttctctgac tggactaact ccctgtcgaa aacgccaatg
     5701 ctgaaagcgc agcacccgga atatgaaacc tggacagcgg gcattcacgg taaaaacaac
     5761 gtgacctgta tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc
     5821 gaccataaaa ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc
     5881 caggacaaag ctgccttgca aaaagtggtt gcggaacgta agcagtcgat taacgacctg
     5941 aaaatcaagg ttgaagatca actggttcac gctcacttcg aagcgaaagc ggcgctggac
     6001 gcaggtgcga cggaagccga aatgaagccg attcaggacg atatccgtca tgcccagtgg
     6061 cgttgggatc tggcgattgc ttcccacggc attcatatgc acgcaccgga agaaggtctg
     6121 aggatgctcg gtactgcgat ggataaagcg gcggatgcac gcaccaaact ggcgcgcctg
     6181 ctggcgacca aaggcatcac ccatgaaatc cagatcccgg atatctcgac caaagagaaa
     6241 gcccagcagg ccattggcct gaacatggaa caaatcaagg ccgagaagca ggacttcatc
     6301 aaaacggtga tcccgcagtg ggaagagcag gcacgtaaaa acggtctgtt aagccaataa
     6361 ccccgttccg cctcgcaagg ggcggaaaac acaatggagt gaatatgagc gtattacgtt
     6421 cgttattaac tgccggggtg ctggcgtcgg gcctgttgtg gagcctgaac ggaattaccg
     6481 ctacccctgc ggcgcaggca tctgatgatc gctacgaagt tacccagcag cgtaacccgg
     6541 atgccgcctg tctggactgt cataagccag ataccgaagg tatgcatggc aaacatgctt
     6601 ccgttatcaa cccgaataac aaactgccgg tcacctgcac caactgtcac ggccagccat
     6661 caccgcaaca ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg atgtacaagg
     6721 ttggagagca gaacagcgtc tgtatgtcct gccatctgcc ggaacagttg caaaaagcgt
     6781 tctggccgca cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc
     6841 cacagcaaga tacgatgcag acgttaagtg acaaaggacg gattaagatt tgcgtcgatt
     6901 gccacagcga tcagcgcacc aatccgaact ttaacccggc gtctgttccg ttgcttaagg
     6961 agcagccatg acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt
     7021 cagcggcacc gcaggacgcg tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg
     7081 catggtgcat gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg cttgtcggga
     7141 agtgaacaag gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg
     7201 cgaatttcct gatgtgaaat atcgcttctt ccgtaagtct tgccagcact gcgatcatgc
     7261 gccgtgcgtt gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta gcgggattgt
     7321 cgatgttaat cctgatctct gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg
     7381 cgtgcgcttt atccatccgg tcacgaagac ggcggacaaa tgcgatttct gccgtaagac
     7441 caatttgcag gccggtaagc tgcccgcgtg cgttgaggct tgcccgacga aggcgctgac
     7501 gtttggcaat ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc agaagcctac
     7561 ttaccgctac aagctggcgc tgggaaccaa accgaagctg taccgcgtac cgtttaaata
     7621 cggggaggtg agccaatgac gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac
     7681 tggccgattg ccatctacct gtttttgatt ggtatttctg ctggtttggt gacgctggct
     7741 gtgctgttac gtcgcttcta cccgcaggcg ggcggtgcag acagcacgtt gctgcgcacc
     7801 acgctgattg tcggacctgg cgcggtgatc ctcggtctgt tgatcctcgt cttccacctg
     7861 acaagaccgt ggaccttctg gaagctgatg ttccactaca gttttacctc ggtgatgtcg
     7921 atgggggtga tgctgtttca gctttacatg gtggtgctgg tgctgtggct ggcgaaaatc
     7981 tttgaacatg atttgcttgc cctgcaacaa cgctggttgc cgaagctggg gatcgtgcaa
     8041 aaggttctga gcctgctgac gcccgttcat cgcggactgg aaacattgat gctggtgctg
     8101 gcggtgttgt tgggggctta taccggtttt ctgctgtcgg cgctgaaatc gtatccgttc
     8161 cttaataacc cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc cggtgcggcg
     8221 gtggcactga tcgccatggc gatacgccaa cgcagtaacc cgcattccac ggaagcgcag
     8281 tttgtacacc gtatggaaat ccccgtggta tggggtgaaa tcttcctgct ggtggcgttt
     8341 tttgtcggtc tggccttggg cgatgacggt aaagtgcgtg cgctggtggc ggcattgggc
     8401 ggcggtttct ggacgtggtg gttctggctt ggtgtcgccg ggctggggct gattgtgcca
     8461 atgttgctca aaccgtgggt caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt
     8521 ggagccagcc tggtcggcgt gttgatgttg cgctttttca ttctctacgc cgggcagtta
     8581 acggtggcgt aagccagaac agaggtggtt tctggacgta ttccttcctg aagtcggttt
     8641 tctggcgctg ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg
     8701 agtgcggttg cgctggcctg ccatgatgcg actcacttgc atcggcattc tggcacagtt
     8761 cgcgctcctg ctgctcgcct ttggcgtact gacgtattgt tttctcatca gcgatttctc
     8821 ggtcatttat gtcgcccaac atagctacag cctgctgtcg tgggaactca aactggcggc
     8881 ggtgtggggc ggtcatgaag gttcgctgct gctttgggtg ctgctgcttt ccgcctggag
     8941 cgcgctgttt gcctggcaat atcggcagca aaccgatccg ctatttccgc tgacgctagc
     9001 cgttttatct ctcatgctcg ccgcactgct actgtttgta gtgctgtggt ccgatccctt
     9061 cgtgcggata tttccaccag caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc
     9121 cggtcttatc tttcatccac cgctgcttta ccttggctat ggcggtttga tggtagcggc
     9181 gagcgtggtg ctggcgagtc tactgcgcgg cgagtttgat ggtgcctgcg cccgaatttg
     9241 ctggcgctgg gcgttacctg gctggagtgc attaacagcg gggatcatcc tcggttcctg
     9301 gtgggcctat tgcgaactgg gctggggcgg ctggtggttc tgggatccgg tggaaaacgc
     9361 ctctttacta ccctggcttt ctgccactgc gttgctgcac agtttatccc tgacacgcca
     9421 gcgggggatt ttccgccact ggtcgctgtt actggcgata gttactctga tgctgtcgct
     9481 gctgggcacc ttaattgtcc gttctggcat tctggtttcg gttcatgcgt tcgcgctgga
     9541 taacgtccgc gccgtgccgt tgttcagtct gtttgtactg attagccttg cgtctctggc
     9601 tctgtatggc tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg ggttatcgcg
     9661 ggaaatgtta atcctcgcta cgctgttgct gttttgcgca gtgctactga tcgtgctggt
     9721 gggaacgctt tatccgatga tttacggcct gctgggctgg ggacgcctct ccgttggcgc
     9781 accgtatttt aaccgcgcga cgttaccgtt tggtctgttg atgttggtgg tgattgtgct
     9841 ggcgacgttt gtctctggta aacgcgcgca gcttccggcg ctgctggcgc atgcgggcgt
     9901 gctgttattt gccgcaggga tcgttgtctc cagcgtcagc cgtcaggaga tcagcctgaa
     9961 tttacagccg ggccagcagg tgatgctggc aggatacacc ttccgttttg agcgcctcga
    10021 tctgcaagcc aaaggcaatt acaccagcga aaaagcgata gtggcgctgt ttgaccatca
    10081 gcaacgcatt gatgaattaa cgccggagcg gcgtttttac gaagctcgtc gtcagcaaat
    10141 gatggaaccg tcaattcgct ggaacggcat ccatgattgg tatgcggtca tgggtgaaaa
    10201 aaccggagcg gatcgttacg cttttcgttt gtatgtacaa agcggtgtgc gctggatctg
    10261 ggggggagga ttgttgatga ttgcaggcgc attattaagc ggatggcggg ggaggaagcg
    10321 cgatgagtaa aggacttctc acgctactgc tgttatttac ctgttttgcg cgcgctcagg
    10381 ttgtagacac ctggcaattc gccaatccgc aacaacagca acaggcgtta aatattgcca
    10441 gccagttacg ttgtccgcag tgccagaatc aaaacttact ggaatccaac gcgccggtgg
    10501 cagtcagtat gcgccatcag gtttacagca tggtggcgga ggggaaaagt gaagtcgaaa
    10561 tcattggctg gatgaccgaa cgctacggag attttgttcg ctataaccca ccgttaacgg
    10621 gtcagacgct ggtgttgtgg gcgctgccag tggtgttgtt actgctgatg gcactgatcc
    10681 tctggcgagt gagggcgaag cgatgaaaca gcctcaaata ccggtgaaaa tgctgacaac
    10741 actcacgata ttgatggtat tcctctgtgt cggcagttat ctgttaagcc caaaatggca
    10801 ggcggtacgt gcggagtatc agcgtcagcg cgatccgcta catcagtttg ccagccagca
    10861 aaccccggaa gcgcagcttc aggcattgca ggataaaatc cgcgctaatc cacaaaacag
    10921 cgaacagtgg gcgttactgg gcgagtacta tctgtggcaa aacgattaca gcaattcgct
    10981 gctggcgtac cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt atgcggcgct
    11041 ggcgacggtg ctttattacc aggccagcca gcatatgacc gcccagactc gcgcaatgat
    11101 cgacaaagcc ctcgcgctgg actctaacga aatcaccgct ctgatgctgc tggcttccga
    11161 tgcgtttatg caggcgaatt acgcgcaagc catcgaacta tggcaaaaag tgatggatct
    11221 caactcaccc cggatcaacc ggacacagct ggtcgagtcg attaatatgg cgaaactgtt
    11281 gcagcggaga tcggattaat catctggtga tgaagattat gttattgagc tgcataaaaa
    11341 ataatcgaat gaacatatgc caaaaataat caccaatcag tattattgca gattaaacaa
    11401 ataaaaatct ttccataaca aatggttatt cattaatcct gccataaact cgcgttatta
    11461 tgcattaatg cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga
    11521 tactgatcat aagcgttaaa aaaatctaca aaccaacgca acacaattca tgccctggca
    11581 gtatgtcacg ttctcgcgtt tctgaacggg gaacggcgct ccattgagga agttattctg
    11641 atgaaaaata taaaattcag cctggcctgg cagattctgt ttgctatggt gctgggcatt
    11701 ctcctgggaa gctacctgca ctaccatagc gacagccgcg actggctggt cgtcaatttg
    11761 ctctctccgg cgggtgatat cttcatccat ctgatcaaaa tgattgttgt gccgattgtg
    11821 atctccacgc tggtggtggg tatcgcgggt gttggtgatg ccaaacagct cgggcgtatt
    11881 ggcgcgaaaa ccattatcta cttcgaggtg atcaccaccg tcgccatcat tttggggatc
    11941 actctggcga acgtcttcca gcccggtgcc ggggtggata tgtcgcagtt ggcgaccgtc
    12001 gatatctcga aatatcagag cactacggaa gcggtacaaa gcagttccca cggcattatg
    12061 ggcacgattt tgtcgctggt gccgacgaac attgtggcgt cgatggcgaa aggcgaaatg
    12121 ctgccgatca tcttcttctc ggtgctgttt ggtctggggc tttcctccct gcccgcgacg
    12181 catcgtgaac cgctggtgac cgtgttccgc tccatctctg aaaccatgtt taaagtgact
    12241 cacatggtga tgcgttatgc gccggtgggt gtgtttgcgc tgattgcggt gacggtggct
    12301 aactttggtt tctcttctct gtggccactg gcgaaactgg tgctgctggt gcatttcgcc
    12361 attctgttct tcgcgctggt agtgctggga attgtggcgc gcctgtgcgg gttaagcgtc
    12421 tggatcctga ttcgtattct gaaagatgag ctgattctgg cgtactccac tgccagctct
    12481 gaaagcgtac tgccgcgaat tattgagaag atggaagcct acggagcacc ggcgtcgatc
    12541 accagcttcg tggtgccgac cggttactct tttaaccttg atggttcgac gctgtatcaa
    12601 agtattgccg ctattttcat cgctcagttg tatggcattg atttgtccat ctggcaggaa
    12661 atcattctgg tgctgacgct gatggtgacc tcgaaaggga ttgctggcgt gccgggcgtg
    12721 tcgtttgtgg tgttgctggc aacgctgggt agcgtaggta ttccgctgga aggtctggcg
    12781 tttattgccg gtgttgaccg tatcctcgac atggcgcgta ctgcgctgaa cgtggtgggt
    12841 aatgcgctgg cggtgctggt gattgccaag tgggaacaca aatttgaccg caaaaaggcg
    12901 ctggcttatg agcgtgaagt gctgggcaaa tttgataaaa ctgcggatca ataattgaag
    12961 attgccgggg ttatcccccg gcaatgtgtg gatgcctgat gcgacgctgg
//
LOCUS       DAZUCE010000137        12956 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZUCE010000137 DAZUCE010000000
VERSION     DAZUCE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.137"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            98..310
                     /gene="cspG"
                     /locus_tag="ACHY03_003336"
     CDS             98..310
                     /gene="cspG"
                     /locus_tag="ACHY03_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFS3906528.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            321..509
                     /gene="ymcF"
                     /locus_tag="ACHY03_003337"
     CDS             321..509
                     /gene="ymcF"
                     /locus_tag="ACHY03_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS3906529.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            484..714
                     /gene="ymcE"
                     /locus_tag="ACHY03_003338"
     CDS             484..714
                     /gene="ymcE"
                     /locus_tag="ACHY03_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFS3906530.1"
                     /translation="MHRWISQNNIRLPCGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            704..877
                     /gene="gnsA"
                     /locus_tag="ACHY03_003339"
     CDS             704..877
                     /gene="gnsA"
                     /locus_tag="ACHY03_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFS3906531.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(926..1999)
                     /gene="yccM"
                     /locus_tag="ACHY03_003340"
     CDS             complement(926..1999)
                     /gene="yccM"
                     /locus_tag="ACHY03_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFS3906532.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFLARPGGIEGWLPIAGLMNLKYSLATGQVPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFTVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(2071..4815)
                     /gene="torS"
                     /locus_tag="ACHY03_003341"
     CDS             complement(2071..4815)
                     /gene="torS"
                     /locus_tag="ACHY03_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFS3906533.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQRQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLSLF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLTTEMADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVDDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQVVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLEHYL
                     QLQVNNDLPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDEIDISRASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            4898..5926
                     /gene="torT"
                     /locus_tag="ACHY03_003342"
     CDS             4898..5926
                     /gene="torT"
                     /locus_tag="ACHY03_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFS3906534.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHICRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5899..6591)
                     /gene="torR"
                     /locus_tag="ACHY03_003343"
     CDS             complement(5899..6591)
                     /gene="torR"
                     /locus_tag="ACHY03_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFS3906535.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6721..7893
                     /gene="torC"
                     /locus_tag="ACHY03_003344"
     CDS             6721..7893
                     /gene="torC"
                     /locus_tag="ACHY03_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFS3906536.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKKNNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7893..10439
                     /gene="torA"
                     /locus_tag="ACHY03_003345"
     CDS             7893..10439
                     /gene="torA"
                     /locus_tag="ACHY03_003345"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFS3906537.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLVGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTIAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            10436..11035
                     /gene="torD"
                     /locus_tag="ACHY03_003346"
     CDS             10436..11035
                     /gene="torD"
                     /locus_tag="ACHY03_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFS3906538.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFVARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(11187..11492)
                     /gene="cbpM"
                     /locus_tag="ACHY03_003347"
     CDS             complement(11187..11492)
                     /gene="cbpM"
                     /locus_tag="ACHY03_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFS3906539.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11492..12412)
                     /gene="cbpA"
                     /locus_tag="ACHY03_003348"
     CDS             complement(11492..12412)
                     /gene="cbpA"
                     /locus_tag="ACHY03_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFS3906540.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGTNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12672..>12956
                     /locus_tag="ACHY03_003349"
     CDS             12672..>12956
                     /locus_tag="ACHY03_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906541.1"
                     /translation="MGSNIHGISCTENNYLKQAWNDIKNEYEKNKTYSITLFENTLVC
                     FIRLYKEIRRQQAEDYIPCLECDSLEKEFEEIQNDNDLSLFMKTLRTNDKQ"
BASE COUNT         3155 a         3284 c         3398 g         3119 t
ORIGIN      
        1 gcattatccc cttcttttga gtctctctcc tgaacactaa gtagtttctg tattaaagcc
       61 ctgtttgccg aaaggcccaa aatgaaggaa gtaaaatatg tctaataaaa tgactggttt
      121 agtaaaatgg tttaacgcag ataaaggttt tggctttatc actcctgatg atggcagcaa
      181 agacgttttc gtccatttca ccgccatcca gagcaatgaa ttccgcacgc tgaacgaaaa
      241 tcagaaagtt gaattttcta ttgagcaggg gcaacgtggc cccgcggcag cgaacgtagt
      301 tacgctctaa ggttgccgtt attactcaac atctccattt tcgctgtcca tgttgtcatg
      361 gttcacagta ccgcacatcg gcattcgatg tgacggagcg aaaccctttg ggcgctaagt
      421 gtattttttg taaatcgacg atgatcacct ttgataacgt tgctctacaa atacgcactg
      481 accatgcacc gctggatttc acaaaataat atcaggctcc cttgtggagc cttttttata
      541 tctgtcttat tttttttcaa cgctgtatgt atagtaagcg ataacctgtt gattattgaa
      601 tctttcgggg agatggctta taacatttct tacctgacca gggtaccggg aaccaacacc
      661 ttactggcgt gttgctgtct tttaagacca gaagaggtta acagtgaata ttgaagagtt
      721 aaaaaaacaa gccgaaacgg aaatcgccga ctttatcgcg caaaaaatcg ccgagctgaa
      781 caagaataca gggaaagaag tctctgaaat tcgcttcacc gcgcgagaaa aaatgaccgg
      841 gcttgaaagt tatgatgtca aaatcaaaat aatgtgattt tgtgaacatc accccgtgcg
      901 aggtgatgtt ccgcttgttg ctaatttagt gaccaatcat tggcgcttgc ggaattaagc
      961 gtcggtacaa ttcctccggc accgggcttt gccatactcc tgcatacatt gcgtaaccaa
     1021 tcaccgtaaa cataatcccc agaaccagta gcgtcattaa ccagccagac aacgcaaagg
     1081 cttttttatt tgccgcaggt ttttgcagtg aaaaggtcaa tgttgaggct accggacatg
     1141 actctacgca agtcatacag ccggtacatt ccactgttcg tacctgaatt aatttatcta
     1201 ccgggatccg tgatgggcaa ttttttgcgc atttgccaca gtcgatacaa ctttcggcat
     1261 tgcgacgaat cttaaacggc gacaatagcg aaaccacgcc catcagcgcg ccatatgggc
     1321 aaagataacg acaccaggca tggcgaataa acaggctggc aatcagcaaa acggtcacgc
     1381 tgattaatgt cgcggtcccc atatgacgaa agaaatcgag cattttaacg tccatcacca
     1441 cgctgtaggg cgacaacata aaatagtgaa tcgcctgagc gggcatcaat aacgcgatat
     1501 agagaaaaaa actcaacagc aaatacttta caccgcgcag aggaatatcc agccagcggg
     1561 gaaggacaca ttgccgacca aacagtttgt taccgagagc gccgattaat tcagaaagcg
     1621 taccaaccgg gcataaccat gaacaaaagg cctttttgag taatagactg atgacgataa
     1681 aagcgaccaa taacagcatc gcggcggcgt ggacggacgg tacctgacct gttgcaaggc
     1741 tatatttcag attcatcaga ccggcaatcg gtagccagcc ttcgatacct cccggtctgg
     1801 cgagaaatgt cgtactacct gccgtttcgt aatagcgcac ccaataccag aacgtgatgg
     1861 caatataaat attcattgcc aatagtaaga attgcgtcgc tttacgccag gtcgtggcat
     1921 tgcgccagtc attccacggt aatttgccgc ccgtcgtgcc tggccgccgc tgccagcggg
     1981 ttcttttttt ctctgccatg attttgccag tccgttaagt tgcataccaa atgccactat
     2041 tctagttatt cttaactggc tgatattgat tcaaatcgcg ttcaggtctt tcttattcaa
     2101 ccatgcttcc agagcggcaa cactgcgtgt aatttcttcg tgtggaaggg gggcagataa
     2161 tggctgctgc tccagttgtg cgcatagctg actggcgcta cgcattccca gactcgagca
     2221 actgcttttt agctgatgcg cggcacgctt tattttctcg ctgtcctggc tggcgcggga
     2281 aatgtcgatt tcatcgagaa gcggcagggc atgttgttta aataatgcca gccattcgtg
     2341 gatcttctcc gtccccatta attgagcatc ttcattgagt tgcgatacat caagtggtag
     2401 atcgttattg acttgcagtt ggagatagtg ctccagtaac tgaccgagca cttcacgcgg
     2461 caccggttta gggataatcc cgcgaaatag tgaactggta cgctggcgca gcgtttcgtc
     2521 aatgacatga gcgctaaagc caatcaaaac cagcgacgga tattgctgtg ccagttgccg
     2581 ggcaagggta atgccgtcga tatccggcag atcaaaatcc accagtgcgg cagcaaacgg
     2641 ttcgctattt tgcagtgtct ctaaagcctg cgcggcattg ccaacagcaa caacctgcgc
     2701 accactggtg ttcagcatct caacggtaat tcgctgggtt agcgggttat cttcaattaa
     2761 cagcaaacgt aaaccgtcaa gacgcaccac ctgattgact gtttttggca cgggtgcagt
     2821 ggcaacacgt aacggcaagc gtaaacaaaa acagcttcca acctccggcg tgctggtggc
     2881 gctcagttcg ccgcccatcg cctgggccag acggctactg atagtcagcc ccagcccggt
     2941 gccgccgcgt ttgccgctta cctgcacaaa tggctggaag atttctgcca gtttcgcggg
     3001 atcaataccg cagccgctgt catccacttc gaccagccat tgttcgccat cagtgcgact
     3061 acgcaggata atgtgccctt cgtcagtaaa acgcagggca ttgctcaaca ggttggtaat
     3121 gacctgacga atacgtcgcg gatcgcccat taacgcagtc ggtacatcgt cggccatttc
     3181 agttgtcagg cgaatcggcc gacctttcac ccgtccgctc attaattgca gggtactttc
     3241 cagcagcggg cgcggttcaa agggctcatc gctgaccgaa acattcttgc cacctgcttc
     3301 gatagcggaa taatcgagaa tatcgttgag gatggtcagc aacgattcgc cggagtcggt
     3361 aattgcccgc aaatcatcac gctgggcgtt aagtgcgggg ttatctgcca gtagctgtgc
     3421 ggtgccgaga ataccgtaca gcggtgtgcg gatctcatgg ctcatcgccg ccagaaacgc
     3481 cgattttgcc tggctggctt tttctgcttc cgcccgtgcc tgtcggtgtt ctatcaccag
     3541 ttcctgcaat tcagctgtac gcgctttgac ctgcgccgcc agctgttcgc ggtggcgatt
     3601 cagtgcatga acactgctgc gaaacgcatc catcagccgc ccgatggtat ccagctcccg
     3661 tacgccagcg gtttccggga aaggggagtc aatatcaccg tccagcagcc gttgcagcgc
     3721 ctgcgtttgt tcggcaagtg gacgcgtgac tgagcgataa accacgcgcc agaggatcag
     3781 aatcagtgcg caaagtgaaa ccatccccag caataacagg ctgtattgcc cgcgtgcact
     3841 ggctttttcc agatgcgcca gtccatgctg attgcgcagc tcaatggtgt cgaccagctg
     3901 actgacttcg ctactaaact gcgcgaactg ggcgatatta ttttgggcga gagtttgcag
     3961 gtgattgctg atttcactgt cctgctggaa cagcgacaac agatcgctat attggctaac
     4021 ggtagttaac gttgttgcga cctgcgcacg aactcccgga tcttcaatgc gtatttgccg
     4081 acgttgcaga attttcaccg cattattgag ctgcttttcc agcgttggtg catttttctg
     4141 gatctgctcc agccccagat tcatcaccat ttgctgcacc cgcagagcgc taaggcgcag
     4201 ttcattcatc tggttaacat actcaagatc gatatcaatc agccgatcga gtgcactttc
     4261 agcagcctga cgctgatctt gttcgatcaa atcgtaaatc ccggcctggg tcgctccagc
     4321 ggaagttgtc gcattattcg cctgaccttg cgccaggcgt gcgatctcat cggcggcagc
     4381 gactatctgc tgactgagtt gctgttgtcg ctggcgtagt tgcagacgtc gccccaccag
     4441 ttccccttgc tgacgtaacg aacgggagat ctcctgctcc tgttgttcaa tagcggtggt
     4501 atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc tttgtgcggt
     4561 gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca ggttctgcgc
     4621 ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca tggtaggaat
     4681 caatgcctga gtgttgtctt tttccacctg gctgataaag cgcaggttgt accatcccac
     4741 cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga gtcttcgggt
     4801 cagggttaaa ttcacggtcg gtgcacttta ggtgaaaaat gttgagtcgc aaagcggaat
     4861 gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc tatttttact
     4921 tctttccctt ttcatgttgc cggcattttc ggctgataac ctgttgcgct ggcatgatgc
     4981 gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat ggaaactgtg
     5041 cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg gtatgcagga
     5101 ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct acagccagtt
     5161 ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg ccattttgct
     5221 cggtagtagc acgacgtcat tccccgatct gcaaaagcag gtagcaagtc tgccggtgat
     5281 cgaactggta aatgctattg atgctcccca ggtgaaaagc cgcgttggtg taccctggtt
     5341 tcagatgggc tatcaacctg ggcgatatct ggtgcaatgg gcgcacggta aaccactgaa
     5401 cgtgctgttg atgcccggac ccgataacgc cgggggcagt aaggagatgg tcgagggttt
     5461 tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgctcg gtgataacga
     5521 tattgaaatc cagcgtaacc tgttgcagga gatgctggaa cgccatccag aaatcgacgt
     5581 cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgca acctgaaaac
     5641 gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc tgaagcgggg
     5701 aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg cggttgagca
     5761 ggccatcagg caattacagg gtcaatcggt gtctgataat gtcagcccac cgattttagt
     5821 tctgacgccg aaaaatgccg accgcgaaca tatctgccgc tcgctgtcgc cagggggatt
     5881 tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt cgccatgttg
     5941 cgtcaccagt aaatccgcgc tgagtttatg gcgtaaacga cgaattaaca catcgacggt
     6001 gcgcaggtca gggttttcca cccgacgcgc agagagcata cgcagcagac gttcacggct
     6061 gagaatttct cccggattgg tcacaaatgc caccaacatt tcatactctg cgcgggtcag
     6121 tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc aataaccggc
     6181 aaagcgatag cagttgtcct gagtgtacgg ttgagcttgt cgcgcgaggt cgattcgcca
     6241 gagcagattt tttacccgta ctaccagttc gcgcagttcc agcggtttgg tgacgtaatc
     6301 gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc cggtaaccag
     6361 aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc cattttcatc
     6421 gggcaagttg atatccagca gaattaaatc taccggctga ttctgcataa tttcccgcag
     6481 cccggcaccg ctcgctgtaa cggaaacggt atacccctcc tgagtgaagt aagattgtaa
     6541 tcgcgcctgg gtaaccggct catcttcaac aataacaatg tgatgtggca tcagagggtt
     6601 ttactcattc tgttcatatc tgttcatatt ctgccgtaag ccgttcatcc tgaccagtgc
     6661 cgctgttcat atttgctcat taagatcgct tcactaaacc ataattttac aggggctatt
     6721 atgcggaaac tctggaacgc gctacgccga cccagtgctc gttggtcggt actggcgctg
     6781 gtcgcaattg ggattgtgat tggcattgcg ctgattgtat tgccacacgt tgggatcaaa
     6841 gtcaccagca caaccgaatt ttgtgtcagt tgccacagta tgcaaccggt gtatgaagaa
     6901 tataaacagt cggtgcattt ccagaacgcc tccggcgtgc gagctgaatg ccatgactgt
     6961 catatcccgc cggatattcc aggcatggtg aagcgcaaac tggaagcgag caatgatatc
     7021 taccagacct ttattgctca ctccattgat acacctgaaa aattcgaagc caaacgcgcg
     7081 gaacttgccg agcgtgaatg ggcgcgaatg aaaaaaaaca actcggcaac ctgccgctcc
     7141 tgccataact acgatgcgat ggatcatgcg aagcagcatc ctgaagcggc acgtcagatg
     7201 aaggtggcag cgaaagataa tcaatcctgc atcgactgtc ataaaggtat tgcccaccag
     7261 ttaccggata tgagtagcgg cttccgtaag cagtttgatg agctgcgcgc cagtgctaat
     7321 gacagtggtg acacgctgta ctctattgat attaagccaa tttatgcggc gaaaggcgat
     7381 aaagaagcct ctggttctct gctgcctgct tcggaagtga aagtccttaa acgtgacggc
     7441 gactggctgc aaattgaaat taccggctgg acagaaagcg ccggacgtca gcgtgtactc
     7501 acccaattcc caggtaaacg catctttgtt gcctcgattc gtggtgatgt gcagcagcag
     7561 gtgaaaacgc tggagaaaac caccgttgcc gacaccaata ccgagtggag caagttgcag
     7621 gccaccgcat ggatgaagaa aggcgacatg gtgaacgata tcaaaccgat ctgggcctat
     7681 gcggattcgt tgtacaacgg cacctgtaac cagtgccacg gcgcaccgga aatcgcccac
     7741 tttgacgcta acggttggat cggcacgctc aacggcatga ttggctttac cagcctcgat
     7801 aaacgtgaag aacgcacctt gttgaaatat ctgcaaatga atgcgtctga caccgcaggt
     7861 aaggctcacg gcgataagaa ggaagaaaaa taatgaacaa taacgatctc tttcaggcat
     7921 cacgtcggcg ttttctggca caactcggcg gcttaactgt cgccgggatg ctggggccgt
     7981 cattgttaac gccgcgccgt gcgactgcgg cgcaagcggc gactgaggct gtcatctcga
     8041 aagagggcat tcttaccggg tcgcactggg gggctatccg cgcgacggtg aaggatggtc
     8101 gctttgtggc ggcaaaaccg ttcgaactgg ataaatatcc gtcgaaaatg atcgccggat
     8161 tgccggacca cgtacacaac gcggcgcgta ttcgttatcc gatggtacgc gtggactggc
     8221 tgcgtaagcg ccatctgagc gacacctccc agcgcggcga taaccgtttt gtgcgcgtga
     8281 gctgggatga agccctcgac atgttctatg aagaactgga gcgcgtacag aaaactcacg
     8341 ggccgagtgc cttgctgacc gccagcggtt ggcaatcgac ggggatgttc cataacgctt
     8401 cggggatgct ggcgaaagct attgccttgc atggtaatag cgttggtacg ggtggggatt
     8461 actctaccgg tgctgcgcag gtgatcctgc cgcgcgtggt tggctcaatg gaagtgtatg
     8521 aacagcaaac ctcctggccg ctggtattgc agaacagtaa aaccattgtg ctgtggggct
     8581 ccgatttgct gaaaaaccag caagcgaact ggtggtgccc ggatcacgat gtttatgaat
     8641 attacgcgca gttgaaagcg aaagtcgccg ccggtgaaat tgaggtcatt agcatcgatc
     8701 cggttgttac atccacccat gagtatctgg ggcgcgagca tgtgaagcac attgcggtta
     8761 acccgcaaac tgacgtgccg ctgcaactgg cgctggcgca tacgctgtac agtgaaaacc
     8821 tgtacgacaa aaacttcctc gctaactact gtgtgggttt tgagcagttc ctgccgtatc
     8881 tggtgggcga gaaagacggt cagccgaaag atgctgcatg ggctgaaaaa ctgaccggca
     8941 ttgatgccga aaccattcgt gggctggcgc ggcagatggc ggcgaacaga acgcagatta
     9001 ttgctggctg gtgcgtacag cgtatgcagc acggtgaaca gtgggcgtgg atgattgtgg
     9061 ttctggcggc gatgctgggg caaattggcc tgccaggtgg tggctttggt tttggctggc
     9121 actataacgg cgcaggcacg ccggggcgta aaggcgttat tctgagtggt ttctccggct
     9181 ctacgtcgat tccgcctgtt cacgacaaca gtgattacaa aggctacagc agcaccattc
     9241 cgattgcccg ttttatcgat gcgatcctcg aaccggggaa agtgatcaac tggaacggta
     9301 aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc cggaactaac ccattccatc
     9361 gccatcagca gatcaaccgc attattgaag gctggcgcaa gctggaaacg gttatcgcca
     9421 tagataacca gtggacctca acctgccgct ttgccgatat cgtgctgcct gcgaccacgc
     9481 agtttgagcg taacgatctc gaccagtacg gcaaccactc caaccgtggc attatcgcca
     9541 tgaaacaggt cgtgccgccg cagttcgagg cgcgcaacga ctttgatatt ttccgcgagc
     9601 tgtgccgccg ctttaatcgc gaagaagcct ttaccgaagg gctggacgaa atgggctggc
     9661 tgaaacgcat ctggcaggaa ggtgttcagc aaggcaaagg acgcggcgtt catttgccag
     9721 cgtttgatga cttctggaat aacaaagagt acgtcgagtt tgaccatccg cagatgtttg
     9781 ttcgccacca ggcattccgc gaagatccgg atctcgaacc gctgggcacg ccgagtggcc
     9841 tgattgagat ctattcgaaa accatcgccg atatgaacta cgacgattgt caggggcatc
     9901 cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc tggctcgcag acgtatccgt
     9961 tgcatctgca atctgtgcat ccggatttcc gacttcactc gcagttatgt gagtcggaaa
    10021 ctctgcgtca gcaatatacg atagcgggta aagagccagt gttcattaac ccgcaggatg
    10081 ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt ctttaacgct cgcggtcagg
    10141 tgttggcagg ggcagtcgtt tctgatcgct atgcacccgg cgtggcgcga attcacgaag
    10201 gggcatggta cgatccagat aaaggcggcg agcctggtgc gctgtgcaaa tacggtaacc
    10261 ccaacgtgtt gaccatcgac atcggtactt cgcagctggc gcaggcgacc agtgcgcaca
    10321 ctacgctggt ggaaattgag aagtacaacg gaacagtgga gcaggtaacg gcgtttaacg
    10381 gccccgtgga gatggtggcg cagtgcgaat atgttcccgc gtcgcaggtg aaattatgac
    10441 cacgctgaca gcacaacaga tagcctgtgt ttacgcctgg ctggcgcagt tgttctcccg
    10501 tgaactggac gatgaacaac tgacgcaaat cgccagtgcg cagatggctg aatggttttc
    10561 gttgctgaaa agcgaaccgc cgctcactgc ggcggtggat gagctggaaa accgtgttgc
    10621 cactctgaca gtacgtgacg atgcccgtct ggaactggcc gctgactttt gcggcctgtt
    10681 tctgatgact gacaaacaag cggcgctgcc gtatgcatcg gcctacaaac aggacgagca
    10741 agaaattaaa cgcttgttag ttgaggcagg gatggaaacc agcggcaatt tcaacgaacc
    10801 ggcagatcat ctggcgatct atctcgagtt gttgagtcat ctgcattttt cgctgggaga
    10861 ggggacggtt cctgcgcgaa gaatcgacag tttacggcaa aaaacactca cggcgctgcg
    10921 gcaatggtta ccagagtttg ttgcgcgttg ccatcagtat gacagctttg gtttttacgc
    10981 ggcactaagc cagttattgc tggtgttagt ggagtgcgac caccaaaaca gataacgtcg
    11041 tttgttggcc tgataagacg cgtttagcgt cgcatcaggc attagggcac cgccgccgga
    11101 tgcggcgtga acgccttatc cggcccacag gaaaagtact ctttgcaggc aggctaagat
    11161 gcgtcatcac atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg
    11221 cgcagcaggc ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc
    11281 gcgatccccg gccagtccag agccagttca tggcgcaggc gcaccgcgcg ttgcaccacg
    11341 atggcggcat ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc
    11401 cccaaaccga caatttcatt caattcctct tcagagatgc cggtatgcag gcaaaattct
    11461 gtaatagtaa aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat
    11521 caaaagacga ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt
    11581 tcggcggcat cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca
    11641 gacctttgcc tttaacgcgc aatcgttgcc cggcctggct gccaggcggg atagtcagca
    11701 aaatgctttc tttcagtgtt ggaacggtga ctttagcacc cagcgccgct tcccacgggc
    11761 taaccggcac cacaatttcc agatcctggc cgacaatatc aaacagcgga tgtggcgcaa
    11821 tatgaatcac cagccacaaa tcgccatttg taccgccgtt ttcgcccggc gtcccctggc
    11881 ctttcaggcg gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt
    11941 tcgggatttc ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg
    12001 tacgcttatg ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt
    12061 ggccgcgtgt ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga
    12121 agatatcgtc aaaatcttcg gcgttaaaac tctgaccgtc gccatggtgg aactgacggt
    12181 taaattgcgg atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat
    12241 cacttaacac ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt
    12301 tgctgacatc aggatggtat ttgcgggcca gtcgacgata ggcggtcttg attgtcttga
    12361 gatcgtccgt cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat
    12421 ctcgcgtaaa tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg
    12481 taatcctcaa aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga
    12541 ggtcgcctca aggtggggtg ctgaacatat tttaaacagg tgaaaaaggg tgagcgattt
    12601 ttgatagttg aaccaggcac tttaagttta actagggcgt cattatttat taaattttat
    12661 aggcgctata tatgggtagt aatatacatg gaattagttg cactgaaaat aattatttga
    12721 agcaagcctg gaacgatata aaaaatgagt acgaaaaaaa taaaacatat tcaatcacgc
    12781 tttttgaaaa cacactggtg tgttttattc ggttgtataa agaaatcaga cgtcaacaag
    12841 ctgaagacta cattccatgc ctggaatgcg attcactaga aaaagaattt gaggaaatac
    12901 agaatgataa tgatttatca ttatttatga aaacattgcg tactaatgat aaacaa
//
LOCUS       DAZUCE010000138        12952 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZUCE010000138 DAZUCE010000000
VERSION     DAZUCE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.138"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(511..936)
                     /gene="arsC"
                     /locus_tag="ACHY03_003350"
     CDS             complement(511..936)
                     /gene="arsC"
                     /locus_tag="ACHY03_003350"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFS3906542.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAKDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGKRLK"
     gene            1470..1553
                     /gene="dinQ"
                     /locus_tag="ACHY03_003351"
     CDS             1470..1553
                     /gene="dinQ"
                     /locus_tag="ACHY03_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFS3906543.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1607..2959)
                     /gene="gorA"
                     /locus_tag="ACHY03_003352"
     CDS             complement(1607..2959)
                     /gene="gorA"
                     /locus_tag="ACHY03_003352"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFS3906544.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(3031..3873)
                     /gene="rlmJ"
                     /locus_tag="ACHY03_003353"
     CDS             complement(3031..3873)
                     /gene="rlmJ"
                     /locus_tag="ACHY03_003353"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFS3906545.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            4076..6118
                     /gene="prlC"
                     /locus_tag="ACHY03_003354"
     CDS             4076..6118
                     /gene="prlC"
                     /locus_tag="ACHY03_003354"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFS3906546.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILTLRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            6126..6878
                     /gene="rsmJ"
                     /locus_tag="ACHY03_003355"
     CDS             6126..6878
                     /gene="rsmJ"
                     /locus_tag="ACHY03_003355"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFS3906547.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6927..8396)
                     /gene="dtpB"
                     /locus_tag="ACHY03_003356"
     CDS             complement(6927..8396)
                     /gene="dtpB"
                     /locus_tag="ACHY03_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFS3906548.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVVNLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            8562..8615
                     /locus_tag="ACHY03_003357"
     CDS             8562..8615
                     /locus_tag="ACHY03_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906549.1"
                     /translation="MVLNIVLAGDFDCERRP"
     gene            8593..8646
                     /locus_tag="ACHY03_003358"
     CDS             8593..8646
                     /locus_tag="ACHY03_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906550.1"
                     /translation="MIANADRRPDKAFTLIR"
     gene            complement(8713..9147)
                     /gene="uspA"
                     /locus_tag="ACHY03_003359"
     CDS             complement(8713..9147)
                     /gene="uspA"
                     /locus_tag="ACHY03_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFS3906551.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9538..9873
                     /gene="uspB"
                     /locus_tag="ACHY03_003360"
     CDS             9538..9873
                     /gene="uspB"
                     /locus_tag="ACHY03_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFS3906552.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9944..11443)
                     /gene="pitA"
                     /locus_tag="ACHY03_003361"
     CDS             complement(9944..11443)
                     /gene="pitA"
                     /locus_tag="ACHY03_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFS3906553.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11675..12877
                     /gene="yhiN"
                     /locus_tag="ACHY03_003362"
     CDS             11675..12877
                     /gene="yhiN"
                     /locus_tag="ACHY03_003362"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFS3906554.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTVRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
BASE COUNT         3222 a         3283 c         3432 g         3015 t
ORIGIN      
        1 ttacctttga cttccctttt atctccaccc ggtaatattt gtgtataaaa aagggcattg
       61 atagtatcgt aaaggaaaac gttaacaact atcaaaaagc gctcatttta aaaagttatg
      121 atgttctccg atagtgtgtg aaatgatatt tttgtgatgt tttagatgtg taataataaa
      181 atgggttaag gtatcttata attattgaat aatttgttgt gtaaagcact gataaatcat
      241 aatccagtac gtctttaaac tgctgcactg tatcgctgat aactaagcag ctcactgata
      301 caccatgctg gacgcgataa ttggaatctc acgcagggag taatgtgtaa atagcaaaat
      361 cgcctgatgc gctacgcctg tcaggcctac aaataacata caattcatcg aattttcata
      421 acattgtagg tcggataaga cgcgccagcg tcgcatccga cataaataaa gcgcactttt
      481 ctaacaacct gtggggggga tatagccgct ttatttcagg cgcttacccg cttcatcaac
      541 cactttctcg ccatcttcct tggtgaatgc gcctttttgc gcatctggca gaatttccag
      601 caccacttct gaagggcggc acaggcgagt tcccagcgtc gtcaccacaa tcgggcgatt
      661 aatcagaatc gggtgctgaa gcataaagtc aattaaccga tcgtcagtaa atttatcttt
      721 tgcaaggccc agttcctcat acggctcgac gtttttacgc agcagcgcgc gtacggaaat
      781 ccccatatcg gcaatgagtt tgaccagttc atcgcgcgtt ggcggagttt ccagataatg
      841 gataatggtc ggttctgtgc cgctgttgcg gatcatctcc agcgtattac gcgacgtgcc
      901 gcaggccggg ttgtgataaa tggtaatgtt gctcatatca ttatctcatt acaaagtgaa
      961 agagagacgt agcgccagcg ccaccagcgt tacaaaccaa agcggataag tcaaaaacat
     1021 atatggttta acgaatgcgt gtgtgcagag gaaggtaata ggtgtgaatt ttgagttggc
     1081 tattcatttg aaaggaggtc tgaatcagga cgtaaaaaaa ccgactttgc gtcggttttt
     1141 tactttccag ccctgagttg gtggctctgg ctggagtgag aatgccatca tctaacgcat
     1201 caatgctaat accatactga aactattgca aggacatgct ggttttataa cctgaatata
     1261 ctgtatgatt atccagttag ctctgaggca ttttcactct ggtaatgcgc ataaacgctt
     1321 acaaagtcct ggtcagaagt acgggtggtg ccgttaactg atgctctggc cggagtgaga
     1381 atgccattat ctaacaatag ggcatgcacc gtgacaggca gtggatgagt aagcggatgc
     1441 attctcactc catcgcatgg agaaaacggg tgattgataa agcaatcatc gttcttgggg
     1501 cgttaattgc gctgctggaa ctgatccgct ttctgcttca gcttctgaac tgatagcgga
     1561 aacgtaatta agggctaaga gcacactact cttagccctt taacatttaa cgcatggtca
     1621 cgaactcttc tgccgccgtt gggtgaatgg cgacggtatt gtcgaagtct tttttggttg
     1681 cccccatctt cagcgccacc gcgaagccct gcaacatttc gtccatacca aaaccaatgc
     1741 cgtgaatacc gacaatcttc tcttctggcc caacgcacac cagcttcatg cggcacggct
     1801 ggcggtgagt ggtgacggcg gtatacatcg cggtgaaaga ggatttatac actttcacct
     1861 gatcgtcgcc gtactgctcg cgcgcctgcg gttccgttaa accaacagta ccaatcggcg
     1921 gatggctgaa gaccacggtc ggaatgttgc tgtaatccag atgctcatcc ggcttgttat
     1981 taaacaggcg ttcagagagg cggcgacccg ctgcaactgc caccggtgtc agttctactg
     2041 cacccgtgtt atcgcccacc gcgtaaatac cttcaatgtt ggtgttttga tatttatcga
     2101 cgacgatata gcctttttcg ttggttttaa cgccagcggc ttcgaggttg atgttgtcat
     2161 tggcaggctc gcgaccaatc gcccaaatca ggcaatccac cgtttcactg cgaccatctt
     2221 ccagctccag cgtcaggcta ccatcggcat ttttcactac cgctttcggg atggcgttgg
     2281 tgtgcaactg cgggccttcg gcgttcatca cttcaaccag cgtttcagag atcatcgggt
     2341 cgaagctgcg cagcggcgca tgtttacgca caaacagatg cgttttcgcg cccaggccgt
     2401 taatcacgcc tgccagttcc acggcgatgt aacccgcgcc aacaaccgcc acgcgctctg
     2461 gcaaagcagg aagggcgaag aagccatcag aatcaatacc gtattccacg cccggaatat
     2521 ccgggtggct cggacgaccg ccggtggcga tcagaatatg atcggctgtg atggtttcgc
     2581 cgtttacttc cagcgttttg gcatcaacga agcgggcaaa gcctttgatt acatcaacgt
     2641 tatttttacc gagcacgttt tcataggaag tatgaatgcg gtcgatatag gcggtacggc
     2701 tggcgatcaa cgtttcccag ttgaatttat tgatagtggt atcgaaacca taatccggac
     2761 cgtacatgtg gatcgcttca cggatttgcg ccgcgtgcca catcactttc ttcggtacgc
     2821 agccgacgtt cacgcaggtg ccgcccagct ctttggcttc aatcagcgcg catttctggc
     2881 cgtacatagc cgcgcggttg atggaggcga taccgccgct gccgccgccg atggcgatgt
     2941 aatcatagtg tttagtcatg acagagtgtc cttatcgttg attaccgcga ttgtagcgcg
     3001 agacgtaata ggtgccagca atggctgcaa ttactctggc acgatccagc ttacggtggc
     3061 gtgcccggtg ccagctggaa ccagtttgct gtgcagccac ggcagcacgt tattcatctg
     3121 ctgttccagc ttccacggcg ggttaatcac aatcatgccg gaagcggtca tgccacggcg
     3181 atcgctgtct ggcagtaccg ccagttcaat ttgcagaatt ttgcgaatgc cggtcgcttc
     3241 caggtcgtgg atcatgcgct taatttgctg gcgtagcacc accggatacc acaatgcgta
     3301 agtgcccgtg gcgaaacgtt tgtaaccttc tgctatcccg ctgactaccg cctgatagtc
     3361 ggttttcatt tcatacggcg gatcgataag gattaaacca cggcgagaaa ccggcggcag
     3421 tttggcctta agctgctgga aaccgtcgga tttttcgacg cgcgcacggc tgtctttctg
     3481 aaattcagaa cgcagcaacg ggtaatcgct cgggtgcagt tcggtcagtt gcaggctgtc
     3541 ctgttcacgc agtaactgac gggcaatcaa cggtgaaccg gggtagtaac gcaactgacc
     3601 gctacggtta aagtgtttta ccacattgat gtacgcctcc agctccgcag gcaaatcgtc
     3661 ctgctgccag atacgggcga taccttcgag atattcgccg gtacgctcgg catgttcgct
     3721 gcctaactga taacgcccgg cccctgcgtg ggtgtcgaga tagagaaacg gtttatcttt
     3781 ctctttcagc gactcaatga tcaggctctg aacggtatgt ttaaggacgt cggcgtggtt
     3841 gccagcgtga aagctgtggc gataactgag catgggtaaa ggtgttccgg taagtaaaaa
     3901 tcggcccgta ttcggggcgc acaaaagcct atcaggacag tataccgaaa agaggccgcc
     3961 gccgcgaaag cgtaacgttt ctcattgaaa ttcactacac ttaaccccat gctacacaca
     4021 ttatgtaaag cgcctgttga gcgcttcctt aacctcttta accaggactg cgctaatgac
     4081 gaatccgtta ctgactccct ttgaattgcc tccgttttct aaaattctcc cggaacatgt
     4141 cgttccagcc gtgactaagg cgctgaacga ctgccgcgaa aatgtggagc gcgtagtagc
     4201 gcaaggagca ccgtacacct gggaaaatct ctgccagccg ttggcggaag tggacgatgt
     4261 gctggggcgt atcttctccc cggtcagcca cctgaactcg gtgaaaaata gcccggaact
     4321 gcgtgaagcg tacgaacaaa ctctgccgct gctgtcggaa tacagcacct gggtagggca
     4381 acatgaaggg ctgtataaag cgtatcgcga cctgcgcgat ggcgatcatt acgccacgct
     4441 gaacacagct cagaaaaaag cggttgataa cgcactgcgc gacttcgaac tctctggcat
     4501 cggtctgccg aaagagaaac agcagcgtta cggcgaaatt gctacccgtc tttctgaact
     4561 gggcaatcag tacagcaaca acgtcctcga tgcgacaatg ggctggacca aactcgttac
     4621 cgacgaagcg gaactggcgg ggatgccaga aagcgcgctg gctgcggcaa aagcccaggc
     4681 tgaagcgaaa gaacttgaag gctacctgct gacgctggat atcccaagct acctgccggt
     4741 aatgacctac tgcgacaacc aggctctgcg cgaagagatg tatcgcgctt acagcacccg
     4801 cgcttccgat caaggtccga acgccggtaa gtgggacaac agcaaggtga tggaagagat
     4861 cctcaccctg cgccacgaac tggcgcaact gctgggcttt gaaaactacg ccttcaaatc
     4921 gctcgccact aaaatggcag aaaacccgca gcaggtgctt gatttcttaa ccgatctggc
     4981 aaaacgcgcg cgtccgcaag gcgaaaaaga gctggcacaa ctgcgcgcct ttgccaaagc
     5041 cgaatttggc gtggatgagt tgcaaccgtg ggatatcgca tactacagcg aaaaacaaaa
     5101 acagcacctc tacagcatca gtgacgaaca gctgcgtccg tacttcccgg aaaacaaagc
     5161 ggttaacggc ctgtttgaag tggttaagcg tatttacggc atcaccgcta aagagcgtaa
     5221 agatgttgat gtctggcatc cggatgtacg tttcttcgaa ctgtatgacg agaacaacga
     5281 actgcgcggt agcttctacc tcgacctgta tgcccgtgaa aacaagcgcg gcggggcgtg
     5341 gatggatgac tgcgtaggcc agatgcgcaa agctgacggt tctctgcaaa aaccggtcgc
     5401 gtatctgacc tgtaacttta accgcccggt aaatggtaaa ccggcgctgt ttacccatga
     5461 cgaagtgatc accctgttcc acgagttcgg tcatggtctg catcatatgc tgacccgcat
     5521 cgaaaccgcc ggtgtatccg ggatcagtgg ggtgccgtgg gatgcggtcg aactgccgag
     5581 tcagtttatg gaaaactggt gctgggagcc tgaggcgctg gcgtttatct ctggtcacta
     5641 cgaaaccggc gaaccgctgc cgaaagagtt gctggataaa atgctggcgg cgaagaacta
     5701 ccaggcggcg ctgtttattc tgcgccagct ggagttcggc ctgttcgatt tccgcctcca
     5761 tgccgagttc cgcccggatc agggagcgaa aatcctcgaa actctggcag aaatcaagaa
     5821 actggttgcc gtagtaccgt ctccatcctg gggccgtttc ccgcacgctt tcagccatat
     5881 tttcgccggt ggttatgccg caggttacta cagctacctg tgggctgacg tactggcggc
     5941 agatgctttc tcgcgctttg aggaagaggg cattttcaac cgtgaaaccg ggcagtcgtt
     6001 cctcgacaac attctgagcc gtggcggttc agaagagccg atggatctgt tcaaacgctt
     6061 ccgtggtcgt gaaccgcagc tggatgcgat gctggagcat tacggcatta agggctgatc
     6121 attcagtgaa aatctgctta attgatgaaa caggcaccgg agacggtgcc ttatctgttc
     6181 tggcggcccg ctgggggctg gagcacgatg aagacaacct gatggcgctg gtgttaacgc
     6241 cggaacatct ggaattgcgc aagcgtgatg agccaaaact tggcggcatc tttgttgatt
     6301 ttgttggcgg agcgatggcg catcgtcgca aattcggcgg tggtcgtggt gaggcggtgg
     6361 cgaaagcggt gggcattaaa ggcgattatt tgccggatgt ggtggacgcc accgcagggc
     6421 tggggcgcga tgcctttgtg ctggcttcag tcggctgccg cgtgcggatg ctggagcgta
     6481 atccggtggt tgccgcgcta ctcgacgacg gcctggcgcg tggttatgcg gatgcggaaa
     6541 tcggcggctg gttgcaggag cgtttgcagt taattcacgc ctccagcctg acggcgctga
     6601 ccgatattac tccgcgcccg caggtggtct atctcgaccc gatgttcccg cataagcaga
     6661 aaagcgcgct ggtgaaaaaa gagatgcgcg tctttcagtc gctggtggga ccggatcttg
     6721 atgccgatgg attactggag cctgcacgct tgctggcgac caaacgcgtg gtggtaaagc
     6781 gcccggacta cgcgccaccg ctggcgaatg tcgccacgcc aaacgcggta gtcactaaag
     6841 ggcataggtt tgatatttat gccggaacgc cagtgtaaaa gtaaagcagg gtagcgataa
     6901 cgctaccctg ctttctgcaa gaataattaa tggctttccg gcgtcgcaat catgcgtttc
     6961 agccacggca ccatcaacag catcactact gcaacgccca gtgtgaccag accaatctta
     7021 ccaaacacgt tggtatagac aggcaacgtc tcaagcggat cggtaatgtt gtctggtact
     7081 gcggtaaatg ttgccacata gccgcccagc aagaacgcgg cagcctgcgt caggaaccac
     7141 atcccgagaa taaagcccat caaatgctgc ggcaccaggg cagcaatcat cgccaggcca
     7201 agggcgctaa taaacagttc acccaggctc tggaacaagt aaaccagcac gataaaccac
     7261 ggcgatgtca gtccttgtgc atcagcaaac cacattcccg cagctgcagc cgtcaaaaag
     7321 cccagcgagc acataaacat accgagagta aatttcatcg gcatcgagag gtctttgcct
     7381 ttgttaccca aatgcgtgta gatacctgcc agtattgggc tggcgagtac cacccagaac
     7441 gggttaagcg cctggaagct gaccgggttg atggaaaagc cgagaatttc atgatgcacg
     7501 ttgttgatgg caaagaagtt cagcgacgtt ggcatctggg cgtagagaat gtaaaacacc
     7561 accgcttcga gcatcaggac aaaggcgaca aacattttat tgcgcccggt tttatccagc
     7621 ttgaatgcct gacgaaagaa gatgatggtg acgacgatgg agagaacaat cagcaccaga
     7681 ttgacgactt ctacgttgtg catcagccag gcacagacga agatcatcac cacgctgcca
     7741 agtaacacgt acaacagttt gctgaagctc atcggccgga agtcgggttc agaaccaatg
     7801 tctttcacca ttccacgaca ggcgatgtaa accagtaatg cgataattaa ccccgccccg
     7861 cacaggttgt aggtgactga atagccgaat ctatcagcga tcacaggggc cagcgataac
     7921 gctatcaacg agccgatgtt gatcgacata tagaacaggg tgaatgcgcc atcaaggcgc
     7981 ggatctttcg gtggatagca cttcgaaagc aagctggctg ggttagcttt aaacaggccg
     8041 ttaccgacag cgatagtccc cagggcgatg aaaatcaggt caggcttaag tagcgacatg
     8101 ccggtcatga agtagccaat cgccagcaca agggctccaa gaacgatggt gcgtttggtc
     8161 cccagcaggt ggtcgccgac atagccgcca atggaaatga ggccatagac cagcgcagca
     8221 aaagcaccaa aagtgacaaa agcctgctct tgcgagaatc caagctgttt aacgaagaaa
     8281 accgccagta cgccctgcac gccgtagtag ccgaatcgct cccataactc gacaaaaaag
     8341 atcatgaaaa atgggcgagg ttgctgcagc atccccatgg gtgttgttgt attcatattt
     8401 attaaccttc caataccatc ctgaaaagta tgaacgcgta agggataacg cgcgaaaaag
     8461 tttacatctg tcagagattc ttaatattga tgcggtgaat agattaatac actattacaa
     8521 cagaaaataa ccagctgatc acactaatga tggaaaatat gatggtgtta aatattgtat
     8581 tggctgggga ttttgattgc gaacgcagac cgtaggccag ataaggcgtt tacgctgatc
     8641 aggtaacaag gcgcgccaaa aagcaaaacg cccgcatatg cgggcgttca ggaacacgtc
     8701 gtggagggaa gattattctt cttcgtcgcg cagcggaaca atcagcatat caacgtgaac
     8761 ggtgttgatc agctgacgtg cggaagacat cagtttgctc cagaagtcct ggtggtgacc
     8821 acaaaccacc agatccatat cgtatttctt gattgcatcg accagaacct ggcccaggtc
     8881 gccgctgccg ctcagggttt cagtgattgg gtagcctgca ttagtggaaa gctcggtcag
     8941 cgcatgatgt gtctcttcag agatgcgttt ctgcatatca ccaagattca catcaataag
     9001 cccggtatat aggtcagagt agtttacatc tacgtggatc agagaaactt tcgcattgta
     9061 ggggcgagcc atagagactg ctttctctac cagaactttg ctttccgggg agagatcgac
     9121 cgcgatgaga atgtgtttat aagccatagt gttactcctt ccataaagtt gtcgatgact
     9181 ggccagctag cgtttcttgt gccgttacgt cacccgcgtc ctgcgaacta cgtgcgcggg
     9241 gctaacccag ccgtaaagat tcagtgttaa gaccatcctt accttatagc gacccggatg
     9301 atccgtcaat ccgccttgct tacccgttag ataaacaaat tttcgacatg aatatattga
     9361 tagtggttaa ccttctggaa aaaaaacaac ctgatctcct acactatcta tagagccgct
     9421 cggatgttgg tcaccacaaa agcggtttct ccaggacctg gtgttaactg attggcagcg
     9481 tatcgggagc ggtagccccg gggtggaact ccgcgggcag gtcgccgggg aggaggtatg
     9541 ataagcaccg tcgcattatt ttgggcttta tgtgtcgttt gcattgttaa catggcgcgc
     9601 tatttctcat cactacgcgc gttgttagtg gtactgcgta actgcgatcc attgctctat
     9661 caatatgttg atggaggggg cttttttacc tcacatggcc aacccaacaa acaggtgcgt
     9721 ctcgtttggt atatctatgc ccaacgttat cgcgatcatc acgatgatga gtttattcgc
     9781 cgctgtgagc gggtgcgtcg gcagtttatt ctgaccagcg cattgtgtgg tctggtggtg
     9841 gtcagcctga ttgccttgat gatttggcat taagcacttc agcaataata acgcataaaa
     9901 aaagcgggcc agtaaactga cccgctcgtt ttggtgtgta cgattacagg aactgcaagg
     9961 agagccagta aagcccgccg gaaagcagta ccgcagccgg aagggtaaac acccaggcca
    10021 tcagaatgct ggtcacggtt ttacgctgta agccgccacc atctaccacc atcgtccccg
    10081 cgacagaaga ggagagtacg tgagtagtgg aaaccggcat cccggtataa ctcgccaggc
    10141 cgatagacac tgccgccgtc atctgggcag acatcccctg agcgtaggtc atgcctttct
    10201 taccgatttt ctcaccgata gtcgttgcca cacgacgcca gccaatcatc gtaccgatac
    10261 ctaacgccag cgcgaccgcc atgatgatcc acaccggtgc atactcgatg gtgctaagca
    10321 tgtcggactt cagtttcttc aacaggcgct gatcgtcagc actcacgcca ggcatcttca
    10381 ccactttgtc gatagtgtca gaaacgcaca gcataatgcg gcgcatctgg ctacgttgat
    10441 caagcgacag cttgtcgtag ctttccacat cggtggtcaa catacctttc aggcggttga
    10501 gcgcgttaat ggtattcgac ggatggcagt ggaactccgc aggttgcgtt gcgccagctt
    10561 ccggagccgg tactaactga tcagcaccgg tagcttgttt cagcagcgca ggatgctgct
    10621 caaagtaagc ttcgacgttg ttaatggcat cacgggtacg ggtgatttcg tagccagtgg
    10681 cattcatgtt caccacgaag cctgctggtg cgacgccaat caataccaac ataaccagac
    10741 caatgccttt ctgaccatcg ttcgcgccgt gcgaaaacgc cacgccgata gcggaaagga
    10801 tcagcgcaat acgcgtccag aacggcggct ttttcttgcc gtctttcttt tcacgctccg
    10861 ctggggtcag gtggatacgg gcgcgtttct tggtgccgct ccagtagcga cgcagcaaga
    10921 aaatcagacc gccagcaaac accaggccga caatagggga aacgatcaga gaaccgaaaa
    10981 tacttaatac tttcgggata ttgagtgcat ccaccactga cgtcccggtc atcaacgcat
    11041 tggttaaacc aatcccgatg atcgcgccaa tcagcgtatg agagctggat gcaggtaaac
    11101 caaagtacca ggtacccagg ttccagataa tcgccgccag caacatagag aacaccatgg
    11161 caaggccatg agacgatccc atattaagca gcagatccgt cggcagcata tgcacaatgg
    11221 cataggcaac actcagacca cccagcaaaa cacccaaaaa gttgaacacc gctgccataa
    11281 ccacggcgag ctgagaacgc atcgcgcggg tatagataac ggttgccacg gcgttggctg
    11341 tgtcatggaa accattgatg gcttcgtaga acagcacaaa agccagtgca agcaataata
    11401 acagcccggt atgcaaatcc aggccagcaa acaaatgtag cataggacgt tacgccattt
    11461 tgaggacatg aacgcggcgc attatcagtg actttcgcgg cgcgggcaaa gtgaaatata
    11521 gacttttttt gatttgcctc ctgtatggat ttcactcaaa aaataattat cttatataat
    11581 tcaggtaaat acttcctttt agtaatattg atgctggtgc gaccactgag gaatctttac
    11641 aattcacgcc cgttttttct aagaggagcg caacgtggaa aggtttgatg ccattattat
    11701 aggcgctggt gcggcgggta tgttctgttc tgcgctggca ggtcaggcag gacgccgggt
    11761 tctgctgatc gataatggta aaaaaccagg gcgcaaaatc cttatgtctg gcggtgggcg
    11821 ctgcaacttt accaaccttt atgtcgaacc gggcgcttat ctgagccaga atccgcattt
    11881 ttgtaagtct gcactcgcgc gttttaccca gtgggatttc attgatctgg tcaataaaca
    11941 cggcatcgcc tggcacgaga aaacgttagg gcaactcttc tgcgatgact ccgcgcagca
    12001 gattgtcgac atgctggtgg atgagtgcga gaagggcaat gtgaccgtca gattgcgtag
    12061 cgaagtgctg agtgtggcga aggatgatac aggcttcacg cttgaactga acggcatgac
    12121 tgtcggttgt gaaaagctgg tcatcgcgac cggcgggctg tcaatgccgg gcctgggcgc
    12181 gtcgccgttt ggttataaga ttgccgaaca atttggcctc aacgtgctgc cgacccgcgc
    12241 gggtctggtg ccattcactc tgcataaacc gttgctcgaa gagttacagg tgctggcggg
    12301 cgtggcggtg ccttccgtga ttaccgctga aaacggcacc gttttccgag agaacttact
    12361 cttcacccac cgcggcttat ctggaccggc ggtgttgcag atttccagct actggcaacc
    12421 gggtgaattt gtcagtatca atctgctacc ggatgtggac ctcgaaacct tcctgaatga
    12481 gcagcgtaac gcacatccga atcagagcct gaaaaacaca ctggcggttc atctaccgaa
    12541 gcggttggtt gaacgcttac agcaactcgg gcaaatcccg gatgtttcgc taaaacagct
    12601 caacgtgcgt gaccaacagg cactgattag cacattgacc gactggcgcg tacaacccaa
    12661 cggcactgaa ggctatcgca ctgccgaagt gacgctcggc ggcgtggaca ctaacgaact
    12721 ctcttcacgg acgatggaag cgcgcaaagt gcctgggctg tactttatcg gcgaagtgat
    12781 ggacgtcacc ggctggctgg ggggctataa cttccagtgg gcgtggtcga gtgcgtgggc
    12841 ttgtgcgcag gatttgattg cagcgaagtc gtcctgagct ttttcctaaa gatgaaaatt
    12901 attagagtcg cattaaaaat gagctaattt tgatagtggc tatcttgtga tt
//
LOCUS       DAZUCE010000139        12933 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZUCE010000139 DAZUCE010000000
VERSION     DAZUCE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.139"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            49..840
                     /locus_tag="ACHY03_003363"
     CDS             49..840
                     /locus_tag="ACHY03_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFS3906555.1"
                     /translation="MSEKLKIVYRPLQELSPYAHNARTHSPEQVAQLVESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEMLKMDSVPVIVLSGLTDEQKKAYRLADNRLPMNAGWD
                     EDLLRMELSDLINADFDVSLTGFIPTEIDELLTDVLPGTGNEEEPYTTKIDTPVYEPS
                     GGKPDISELYDDTKTQELISRIRSASLEPDIEKFLLCAAERHTVFNFSRIADYYAHAP
                     AEIQCFFEESALVIIDYQQAIENGFVRMTQRMVEIMHGGEEEEYA"
     gene            833..1765
                     /locus_tag="ACHY03_003364"
     CDS             833..1765
                     /locus_tag="ACHY03_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906556.1"
                     /translation="MRDDFCAFILTHGRPDKVLTYRTLRRAGYTGKIFIVVDDEDKTR
                     HQYMAEFGEQVLVFSKADIASRFDEADNFCDRRSIFYARNACFDQAKLVGCKYFIQLD
                     DDYHEFQFRVDRNYDQAYFPIRKLDAILSEMLAYYESIPALSIAMSQGGDFLGDNGGH
                     ASWVKRKAMNSFICSVDRPFSFMGRINEDVNTYTNLGRCGELFMTIGAVQLGQKQTQK
                     NSGGMTELYLDSGTYVKSFYSVMYAPSCVKISLMGASHKRIHHQVTWNNAAVKILHEK
                     YRKKTPCISIGVTNDSVFESRVSGSVPDDCTTNR"
     gene            1743..1952
                     /locus_tag="ACHY03_003365"
     CDS             1743..1952
                     /locus_tag="ACHY03_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906557.1"
                     /translation="MTAQQIADVLDVDLNRLKENREAMTDFYAAIRKGRAKGEAELRA
                     ALFKLARKGDAFALRELLRLDKNQD"
     gene            1956..3050
                     /locus_tag="ACHY03_003366"
     CDS             1956..3050
                     /locus_tag="ACHY03_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS3906558.1"
                     /translation="MSRPDWGALQQEYIAEYTRSGISPVAWCEARGLNYATARRYIKK
                     PPKNAQTEMRKTAQKSAQKKSAQTAQKRNEKSQEKKPVFDAGLNEGDAEEFSFCPDEF
                     GISDQQAKFAMLVAQGKKPTEAYRLAGYEGQGATANSNASRMLRNARVYRAISYFRNQ
                     YQKRYTADLDLLVSQLMAIVQADPNQLAQFRRVNCRYCWGENHLYQWRDIAEFDKAAA
                     QASRDGKPEPEYGGLGFVDNAIPNPDCPKCCGEGTGQLYMADTTLLDGDARQLYAGSK
                     LGKYGVEILLEDKAAARRELLRLLSAGGALCADKRLQELEIERRRIENQKLRKEIETV
                     EDNEHPQPVAININVVDARVRSDEDDLSDA"
     gene            3031..4332
                     /locus_tag="ACHY03_003367"
     CDS             3031..4332
                     /locus_tag="ACHY03_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFS3906559.1"
                     /translation="MISPTLNVPQARFLSMPHKFKAYIAGFGSGKTWVGCGGICKGIW
                     EHPGINQGYFAPTYPQIRDIFYPTVEEVAADWGLNVKINEGNKEVHFYYGRQYRGTTI
                     CRSMEKPQTIVGFKIGNALVDELDILPKEKARTAWRKIIARMRYKIDGLRNGIDVTTT
                     PEGFKFVYEQFVKAVREKTELASLYGLVQASTFDNEKNLPADYIPSLLESYPPELIKA
                     YLRGQFTNLTSGTVYHQFDRKLNNCEEVEQPGEPIYIGMDFNVGKMAGIVHVLRLGLP
                     CAVTEIINAYDTPDMIRIIKERFWLYDGNDYRKVREIYIYPDASGDSRKSSNASTTDI
                     AQLKQAGFNVVVNSSNPPVKDRVNSMNAMFCNANGERRYKVNVKRCPLYAESLEQQVW
                     DEKGEPDKKSGNDHPNDAGGYFIVKQFPIVKPTGRVTSLRI"
     gene            4335..5741
                     /locus_tag="ACHY03_003368"
     CDS             4335..5741
                     /locus_tag="ACHY03_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HFS3906560.1"
                     /translation="MADISTPNLDYNDMLEAWDINDALMGGTLEMRRQGENYLPKWPN
                     EDEDAYKKRLSVATLLPVYEESIKQNIGRIFAEPTVLSEETPAKIREYAENIDMEGSR
                     LDVWAQQFFSLAFQYGVAHALVDYPRTDMKEIRTKADENATGGRPYVTMLNPRQVIGW
                     KSKVEKGKVVLTDLRIKEVIIIDGDDFGQKKVEQIRHIMPRRVEIYRRSEGTNGESVW
                     TLHESWNTSRDDIPLVTLYTKKTGFMRGTPPLLNLGLLNIKHWQSQSEQDNILHVARV
                     PLLVAYGLDRNEELTVGASTATIFEDRTKNGLEYVEHSGAAIESGETSLEKLENQMRH
                     AGAKLLRAENTSTKSVDQTNEERMQENSPLYTMANSLEDALDNILQIMAEWIGESCGG
                     NVDVRTELDVSAQVFDSSSALAVQSLRQGGDIRQIDAVRVLQALKFIDQDSRPEEVID
                     ELKNQSVMLMEINDANRE"
     gene            5725..6837
                     /locus_tag="ACHY03_003369"
     CDS             5725..6837
                     /locus_tag="ACHY03_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFS3906561.1"
                     /translation="MQTVNERLRDESIAHAVWISRYSTGVAARMVKILNDSDAELTAR
                     LLVALDSLDPGSFTVTRLESLLASVREVNRAAINSMFTRLSGELNELAIYEAGYQLSL
                     FDSMLPDFVADVHPLVGISPDALYAAAMARPFQGRLLSEWASDLEADRLRRITNTVRQ
                     GFLLGDTNEQIARKIRGHVSKGFQDGALQMSRANAASIAKTAVGHLAATARESFASAN
                     NDLIKGKQWLSTLDNRTTPQCRIRDRLKYTLNNKPVGHSVPYLQGPGKIHFCCRSTET
                     FILKSAKELGIDVRDISPAERASMDGVVAGDTTYREWFLRQPYTRQKQIVGETRAKLI
                     RDGGMSPDEFYTDKGEWLTLKQLRERDAQVFRKAGI"
     gene            6942..7706
                     /locus_tag="ACHY03_003370"
     CDS             6942..7706
                     /locus_tag="ACHY03_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6651 domain-containing protein"
                     /protein_id="HFS3906562.1"
                     /translation="MKLKTVEINGKQYAEIDTAGLPVYVHDDGKEIGFDAPLATKKIT
                     ELNGEAKNHRLAKEAAEEKLAKFAAIEDPKKAIEALEMLSKIDQKKLLDAGQVDQVKA
                     EITKNFQQQLDEEKQRSQMLETQLYDSMIGGSFAGSKYIADKIAIPADLLQARFGQAF
                     KVEEGKIVAYDASGNKIYSRAKPGELAQFDEALEFLVENYPQKDYILKASGNNGGGSR
                     PTQHDIGQKTMKRSAFDALDIAGKQNALKDGITIVD"
     gene            7805..8944
                     /locus_tag="ACHY03_003371"
     CDS             7805..8944
                     /locus_tag="ACHY03_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFS3906563.1"
                     /translation="MAGNTLTGLIPTIYTALDIVSREQVGFIPAVAKNAKADAAAKDQ
                     TVTAPVAPEAKTEDIVPGPSAPNTGDQNIGTVDVKITKSKMAPVKWNGEEQLALGPSG
                     TYNTILADQFKQAFRALANEVEADLGALYFGASRAVGTAGTTPFGVKDDLSDAALARQ
                     VLEDNGAPTTDLQMVLGSTAIANLRGKQSVLFKVNESGTEQLLREGVLGRLEGFNIHS
                     SAGVKRAPKVAATGYLVNGEKKEGDVLISIDTGSGSISAGQIVTFAGDPNQYVVAAAT
                     SNLITLAAPGLRQDLADDTAITVVGSFTANMAFDRNAFLLASRTPAMPEGGDNADDVM
                     NVTDPISGITFQIALYRQYRQVRYEVGLAWGVSSVKPAHGCLILG"
     gene            8987..9163
                     /locus_tag="ACHY03_003372"
     CDS             8987..9163
                     /locus_tag="ACHY03_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004121546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906564.1"
                     /translation="MAGLTKEQRAQREAAQKTQIELVVMVTDYQMFPGAPTIANVHPD
                     EVDNWKAMGWKTQE"
     gene            9167..9562
                     /locus_tag="ACHY03_003373"
     CDS             9167..9562
                     /locus_tag="ACHY03_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein singed"
                     /protein_id="HFS3906565.1"
                     /translation="MITYVTCDDVDNAFGNGWTSENAKNKAVLMANAWLNGFNLKINP
                     SRIPEEVKLAGAYAARIASFGKLFQQKNDSGVVISKAVSADGVSVSKSFAELPANSTA
                     LLEPDLQLAIALLKPYGLSRSQVRVVRGG"
     gene            9562..9945
                     /locus_tag="ACHY03_003374"
     CDS             9562..9945
                     /locus_tag="ACHY03_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS3906566.1"
                     /translation="MGLREEIQSEVAAAFDEDLADAVSDFSGSYVTHRHWDPVTETGG
                     ESTAIYTGRGVLTRYKLGRIDGINILHGDLKLTALVCEVTDKPAVDHIIEIYDPVLRQ
                     LQRYEVITASVDPSASVYSIQLRRA"
     gene            9946..10326
                     /locus_tag="ACHY03_003375"
     CDS             9946..10326
                     /locus_tag="ACHY03_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFS3906567.1"
                     /translation="MAKAWDIEPSIFAGMIEEDVGLKIRYIAMQILTAIDIAAPVDTG
                     RFRNNNLVSLQHPDFGISDNVDPNGTIAVQRGIGVISKAANYGVIYIQNNLPYAEALE
                     NGHSQQAPTGVYANAFHGVLQAYK"
     gene            10323..10715
                     /locus_tag="ACHY03_003376"
     CDS             10323..10715
                     /locus_tag="ACHY03_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator-like protein"
                     /protein_id="HFS3906568.1"
                     /translation="MTFTEIRNTVISRMTAQTIIDGKDVLYPNGPTFDPSGKLIWARL
                     SNIPGQAGVNEIGAGPVVYRTGIIIIQLFVPAGSGSKLITETADKLRELFEFQDDDRL
                     SYQAVSSIAVGEKNGWFQLNLQIPYRAL"
     gene            10742..11704
                     /locus_tag="ACHY03_003377"
     CDS             10742..11704
                     /locus_tag="ACHY03_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFS3906569.1"
                     /translation="MSSGAKVISAFIRETVAGTTPASGDWSLLKRTSWGVKPTQNKGE
                     NNEIGGSRMAQGATPGTVDVGGDVGTKFRWGQHDDFLASCFGAEWSGDSLTMGNERIT
                     FSLATYASDVGIASVVRGAQVGSWKMQIPNDGDITATVTFAGLDWESKADDTNFIKGE
                     PVDSAGKLRYSFKEVSAVSLNGVAGGNGFCIDSFDIQFDNKLQTQRCIGTGSPYAGAN
                     IPTTFTPSGTVTLSWSKAAWEIWSKTLTGETVPFSFTLSNGEGAYTFSFPKVQVSGEW
                     PDGGNSDIIQVQLSITAADEAPTITRKKCSPTAVIAKASADAIS"
     gene            11855..12214
                     /gene="ydaY"
                     /locus_tag="ACHY03_003378"
     CDS             11855..12214
                     /gene="ydaY"
                     /locus_tag="ACHY03_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdaY"
                     /protein_id="HFS3906570.1"
                     /translation="MVASSDNDQYRSRNALIRRHIEKMDASLHVGTKEFDISKVSEVD
                     SVDDLLIDNAARYLLKDWKGVGELVNGAEVALEYTPERGIALLKQNPELYWQILAEAA
                     SIAQGKEQQKQDTIKKP"
     gene            12322..12522
                     /locus_tag="ACHY03_003379"
     CDS             12322..12522
                     /locus_tag="ACHY03_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906571.1"
                     /translation="MLKELLYAYSVISRARRYAGMTGVPLPLSLTEINEYLATHPVLI
                     ERDEFEAVIFALDDQYFQDQCV"
     gene            12686..>12933
                     /locus_tag="ACHY03_003380"
     CDS             12686..>12933
                     /locus_tag="ACHY03_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS3906572.1"
                     /translation="MAEQTSRLAIIIDSTGAKNNADNLTSSLVKMTQAGETAANSAGK
                     VTKATEDEKNALAKLKAAIDPVGAAIDTVGRRYSELKK"
BASE COUNT         3502 a         2703 c         3501 g         3227 t
ORIGIN      
        1 cagtagcact ggctgctggg gtgcgtttta ttcataaagc aaggctgtat gagcgagaaa
       61 ttaaagatag tctatcgccc attacaagaa ttgtcaccgt atgcgcacaa cgccaggacg
      121 cacagccctg agcaggtggc acaactggta gaaagtatta agcaattcgg ctggactaat
      181 ccggtgctga ttgacgaaaa gggcgaaatt attgcgggtc acggtcgtgt tatggcggct
      241 gaaatgctca aaatggattc tgttccggtc attgttctgt ctggcctgac ggatgagcag
      301 aaaaaggcgt accgcctggc agataatcgc ctgccgatga atgctggctg ggatgaagat
      361 ctgttgcgga tggagctgtc ggacctaatc aatgctgatt ttgatgtctc cctgacaggc
      421 ttcatcccga cagaaattga tgaactgttg acggatgttt tgcccggtac aggaaatgag
      481 gaggagccgt atacgacgaa aattgatacg cctgtttatg agccgtcggg cggtaaaccg
      541 gatatcagtg aactgtacga cgatacgaaa actcaggagc tgatcagccg gatacgttcg
      601 gcgtcccttg agcctgatat tgagaaattc ctcctgtgtg cggcagaacg tcacacggtg
      661 tttaatttca gcagaattgc ggactattac gctcacgccc ccgctgaaat tcagtgtttt
      721 ttcgaggagt cggcgctggt gatcattgat tatcagcagg ctattgaaaa tggatttgtc
      781 cggatgacgc agcgcatggt ggagatcatg catggtggtg aggaggagga atatgcgtga
      841 tgatttttgc gcctttattc tgactcacgg gcgaccggac aaagttctga cttaccggac
      901 gttgcgtcgt gctggctata ccgggaaaat ttttatcgtt gttgatgatg aagataagac
      961 acggcatcag tacatggctg aatttggtga acaggtgctg gtgttttcca aagccgatat
     1021 cgccagtcgt tttgacgaag ccgataattt ctgtgaccgc cgctcaattt tttacgcccg
     1081 taatgcctgt ttcgaccagg caaaactggt cgggtgtaaa tacttcattc agctcgatga
     1141 tgattatcac gagttccagt ttcgggtgga tcgcaactat gaccaggcct attttccgat
     1201 aaggaaactg gatgcgatcc tttctgaaat gctggcgtac tacgaatcaa tacctgctct
     1261 ttccatcgct atgtcgcagg gaggggattt tcttggtgac aatggcggcc atgcttcgtg
     1321 ggtgaaacgc aaggcaatga acagctttat ctgttcggtt gatcgaccgt tctcattcat
     1381 ggggcgcatt aacgaggatg tgaatacgta cacgaatctc ggtcgctgtg gtgaattgtt
     1441 tatgacgatc ggtgctgtcc agttagggca gaaacagacg cagaaaaaca gcggcggaat
     1501 gaccgagctg tatctggatt ccggaaccta cgttaaaagt ttttactccg tcatgtatgc
     1561 gccgtcgtgc gtaaaaatct cactgatggg tgccagccat aaacgcattc accatcaggt
     1621 cacctggaac aacgctgcag taaaaatcct tcacgaaaaa tacaggaaga agacaccctg
     1681 catatcaata ggggtgacaa atgattccgt attcgaaagt cgagtctctg gcagcgtgcc
     1741 ggatgactgc acaacaaatc gctgacgttc tggatgttga tctgaaccga ctgaaagaaa
     1801 atcgggaagc aatgacagat ttttatgcgg ccatccgtaa gggcagagcg aaaggtgaag
     1861 ccgagttacg agcggcattg tttaagcttg ccagaaaagg ggatgccttt gctctgcgcg
     1921 aactactcag gttggataaa aatcaggact aactgatgag cagaccggac tggggggcgt
     1981 tgcagcagga gtatattgct gaatacaccc gctccggtat atctccggtg gcatggtgtg
     2041 aagcaagggg actgaattac gcaacagccc gtcgttacat caaaaaacct ccgaaaaatg
     2101 cgcagacaga aatgcgcaaa actgcgcaaa aaagtgcgca gaaaaaatct gcgcagactg
     2161 cacaaaagcg gaacgaaaaa tctcaggaaa aaaagccagt attcgatgcg ggcctgaatg
     2221 agggcgacgc ggaggaattt tcgttctgtc ccgatgaatt cggcatttct gaccagcagg
     2281 ctaagtttgc gatgcttgtt gctcagggta aaaagccgac agaggcgtac cgactagccg
     2341 gttatgaggg gcaaggtgcg acagctaaca gcaacgccag tcgtatgctt agaaatgcca
     2401 gggtttatcg tgctatcagc tacttccgca atcagtacca gaaacgctat accgcagacc
     2461 tggatttact ggtgagccag ttgatggcca ttgtccaggc cgaccccaat cagttggcac
     2521 aatttcgccg tgttaactgc cgttattgct ggggcgagaa tcatctctac cagtggcgtg
     2581 atattgcaga attcgataag gctgcggcac aggcctccag agatggtaaa cccgagccgg
     2641 aatatggagg cctcggcttt gttgataacg ccatacccaa tccggattgc ccgaagtgct
     2701 gcggtgaggg aacgggacag ctttatatgg ctgataccac actgcttgat ggggatgcgc
     2761 ggcaattata tgcagggtca aagctcggaa aatacggtgt tgagatcctg ctggaggata
     2821 aggctgccgc ccggcgtgaa ttgttgcgtt tgctttctgc tggcggggca ttatgtgcag
     2881 ataagcggct acaggaactg gaaattgaac ggcgcagaat agaaaaccag aagctgcgca
     2941 aagagatcga aacggtggag gataatgaac atccccagcc tgtggcgatc aatattaatg
     3001 tggttgatgc cagagtaagg agtgatgaag atgatctctc cgacgcttaa tgtgcctcag
     3061 gcgcgatttc tttcaatgcc ccataaattt aaagcctata ttgctggttt tggctcgggc
     3121 aaaacatggg ttgggtgtgg cggcatatgc aaggggattt gggagcatcc aggtataaat
     3181 cagggatatt ttgcgccaac gtatccccaa attcgcgata ttttttaccc tacagtggaa
     3241 gaagttgctg ctgactgggg attgaacgta aaaattaatg agggaaataa agaggttcac
     3301 ttttattacg gacgccagta tcggggaacc actatctgca gatcgatgga gaagccacaa
     3361 acgatcgtcg gtttcaaaat tggtaatgcg ttggtggatg aactggacat tttgccgaag
     3421 gaaaaagcca gaacggcgtg gcgcaagata attgcacgta tgcgttataa gattgatgga
     3481 cttcgcaacg gtattgacgt tacaaccacg ccggaaggat tcaaatttgt ctacgagcag
     3541 tttgttaaag ccgtgcgtga aaaaacagag ctggcctcac tgtatggtct ggtgcaggca
     3601 tctactttcg ataatgaaaa gaacctgcca gcagattaca ttccttcgct tcttgaatca
     3661 taccctccag agctgattaa agcctatctt cgaggacagt ttactaacct gacaagtggt
     3721 actgtttacc atcagtttga ccggaaactg aataattgcg aagaagtgga gcagccaggg
     3781 gagccgattt atattgggat ggattttaac gttggaaaga tggcgggaat cgtccatgtg
     3841 ctgcgtttgg ggcttccatg tgcggtaact gaaatcatca atgcctacga tacgccggat
     3901 atgatccgca tcattaaaga acgcttctgg ctgtatgatg ggaatgatta ccggaaagtg
     3961 agggaaattt atatttatcc agatgcttcc ggggattcca gaaaatcaag taacgcaagt
     4021 acgacggata tagcccagct taagcaggct ggttttaacg ttgtggtgaa cagctcgaac
     4081 ccgccagtaa aagatcgcgt taactcaatg aatgcaatgt tctgcaatgc caatggtgag
     4141 cgtcgctata aagttaatgt gaagcgttgc ccgttatatg ccgaatctct ggagcaacag
     4201 gtctgggatg agaaggggga gcctgataaa aaatctggca atgatcaccc gaatgatgcc
     4261 ggaggttatt tcatcgttaa gcaattccct attgtcaaac cgaccggaag agtcacatca
     4321 cttcggattt aattatggct gatatatcaa cacccaacct cgactataac gatatgctgg
     4381 aggcgtggga tattaacgac gcactgatgg gcggtacgct tgaaatgcgc aggcaggggg
     4441 aaaattatct cccaaaatgg cctaacgaag atgaagacgc ttataaaaaa cgcctgtctg
     4501 tggctacgct acttcctgtg tatgaagaaa gcatcaagca aaatattggg cgcatatttg
     4561 cagagccgac agtattgagt gaggaaacgc cggcaaaaat cagggaatat gcagaaaata
     4621 tcgacatgga ggggagccga ctggatgtgt gggcgcagca atttttcagc ctcgcatttc
     4681 agtatggtgt ggcacatgcg ctggtggact atccacgaac ggatatgaaa gagattcgga
     4741 caaaagccga tgaaaacgcg accggtggtc gtccgtacgt tacgatgctg aatccacgcc
     4801 aggttattgg atggaaatcg aaagttgaaa aagggaaagt tgttctcact gatttgcgta
     4861 taaaagaggt catcattatt gatggtgatg atttcgggca gaagaaggtg gagcagatcc
     4921 gccatattat gccccgtcga gttgaaattt atcgacgtag cgaaggcact aatggcgaat
     4981 ctgtctggac gcttcatgag tcatggaata ccagccgtga tgatattcct ctggtaacac
     5041 tgtacacgaa gaaaacagga tttatgcgtg gtacaccgcc attgcttaat cttggcttgc
     5101 tgaatatcaa gcactggcaa agccagagcg agcaggacaa tattcttcat gttgcccgcg
     5161 ttccattgct ggtggcctac gggctggaca ggaatgaaga actgacggtt ggtgcatcca
     5221 ccgctacgat ttttgaggac agaacaaaaa atggcctcga atatgttgaa catagtggcg
     5281 cagcgataga atctggcgaa acatcacttg agaagctgga aaatcagatg cgtcatgccg
     5341 gcgctaaact tctgcgtgct gaaaatacat ccaccaaatc tgttgatcag actaatgaag
     5401 agcgaatgca ggaaaactcg ccgctttata caatggcgaa ctccctcgaa gatgccctcg
     5461 ataatattct ccagataatg gcggaatgga tcggagagag ttgcggtggc aatgtggatg
     5521 tgcgcactga actggatgta tctgcccagg tgtttgactc atcctccgcg ctggctgttc
     5581 agtcattgcg tcagggcggt gatatacgtc agattgatgc ggttcgggtg ttgcaggcgt
     5641 tgaaatttat cgatcaggat tcccgtccag aggaagtgat cgatgaatta aaaaatcaga
     5701 gtgtaatgct gatggaaatc aatgatgcaa accgtgaatg aacggctgcg tgatgaatca
     5761 attgctcatg cagtctggat atcccgctat agcactggtg tggctgccag aatggtgaaa
     5821 atactgaatg acagcgatgc ggagcttaca gctcgcctgc tggtagcact ggacagcctc
     5881 gatcctggta gttttaccgt tacgcgcctg gagtcacttc tggcgagtgt cagagaggtt
     5941 aaccgcgctg ctattaacag catgtttacc cgtctctccg gagagctgaa cgagctggca
     6001 atttatgagg ctggttatca gttaagtctg tttgattcta tgctacctga ttttgttgct
     6061 gatgttcacc ctctggttgg tatctctcct gatgcacttt atgccgctgc aatggcgcga
     6121 ccattccagg gacgactgct cagtgagtgg gcctcagatc ttgaggcgga tcggctcaga
     6181 cgcataacaa atacagtgcg tcagggtttt ttgctggggg ataccaatga gcagatcgca
     6241 agaaaaattc ggggacatgt cagtaaggga tttcaggatg gtgcattgca gatgagccgg
     6301 gctaatgcgg ccagcattgc aaaaacagcg gttggacatc ttgctgctac tgcccgtgag
     6361 agttttgcca gcgcgaataa tgatttgatt aagggtaagc aatggttatc aacgcttgat
     6421 aatcgtacta cgccacaatg tcgaatcagg gatcgcctca aatatacgct gaataataaa
     6481 cctgtaggtc acagcgtgcc ttatttgcag gggccgggaa aaattcattt ctgctgccgc
     6541 tcaacggaaa cgttcattct gaaatcagcg aaggaactgg gtattgatgt tcgcgatatt
     6601 tccccggctg agcgggccag catggatggc gtggtggccg gagatacaac ctacagggaa
     6661 tggtttttgc gtcagcctta caccagacaa aaacagattg tgggggaaac ccgagcaaag
     6721 ctgattcggg atggtggtat gtcgccagat gaattttaca ccgataaagg cgaatggctg
     6781 acgctgaagc agctccgtga gcgtgatgca caggtattca gaaaagcagg gatttaaata
     6841 aatcatttat tacaacaggc taccttcggg tggccttttt tattgctgcg atccggatgg
     6901 tgagcagcgt aactgtcgga agacttaaac caggtactaa tatgaaactg aaaacggtcg
     6961 agattaacgg aaaacaatac gcagaaattg atactgctgg cctgccagtt tatgtgcacg
     7021 acgatggtaa agaaattggc ttcgatgcac cgctggcgac aaaaaaaatt acagagctta
     7081 atggcgaggc aaaaaatcat cgcctggcta aagaagctgc agaggaaaaa ctggctaagt
     7141 ttgccgctat cgaagacccg aagaaggcga tcgaggcact ggaaatgctg tcaaaaatcg
     7201 accagaaaaa gctgctcgat gcgggacagg ttgaccaggt taaggcagaa attacgaaaa
     7261 attttcagca gcaattagat gaagaaaagc aacgctctca gatgctggag acgcagcttt
     7321 acgattctat gattggcggt agttttgcgg gttcaaaata tattgccgat aaaattgcga
     7381 tcccggcaga tttattacaa gcccgcttcg ggcaggcatt caaagtggaa gaagggaaga
     7441 tcgttgctta tgacgcttcc ggcaacaaaa tttattcccg cgcgaagcct ggcgaactgg
     7501 cgcagtttga tgaggcgctg gagttcctcg tcgaaaatta ccctcagaaa gactacatcc
     7561 tgaaagccag tggcaacaat ggcggcggct cccgtccgac acagcatgat attggtcaga
     7621 aaacgatgaa acgctctgct tttgatgcac tggatattgc aggtaagcaa aacgcattga
     7681 aagacggtat cacaattgtt gattaacaca tttgtcagct tccggatggg agctggtgtc
     7741 agggctggat agctcattac tccatctatt cacaattacg caaattttta aggaatattt
     7801 aattatggct ggaaataccc tgaccgggtt gatcccgact atttataccg ccctggatat
     7861 tgtatcccgt gagcaggtag gttttatccc tgcggtagca aaaaacgcaa aagctgacgc
     7921 cgcagcaaaa gatcagacgg taaccgcgcc agttgcgcct gaggcgaaaa ccgaagatat
     7981 cgtaccgggg ccgtcagctc cgaataccgg tgatcaaaat attggcactg ttgatgtgaa
     8041 aattactaaa tccaaaatgg caccggttaa atggaatggt gaagaacaac tggctcttgg
     8101 cccttcaggg acttacaaca ccattcttgc tgatcagttc aagcaggctt ttcgcgccct
     8161 tgcaaacgag gttgaggctg atcttggtgc gttgtatttc ggtgcttccc gcgccgtggg
     8221 aaccgcaggg acaacgccgt ttggtgttaa agatgatctt tctgatgctg ctctggctcg
     8281 tcaggttctg gaggataacg gcgcaccgac aaccgatctg cagatggtgc ttggctccac
     8341 tgccattgct aatttgcgcg gaaaacagtc tgtactgttt aaagtgaatg aatccggcac
     8401 tgaacagcta ctgcgtgagg gcgtgttggg gcgtctggag ggattcaata ttcacagttc
     8461 ggcaggtgta aaacgagcgc caaaggttgc tgcaactggt tatctcgtga atggcgagaa
     8521 aaaagaaggc gatgttctta tttccattga tactggttcg gggagtattt ctgcaggtca
     8581 gattgttacg ttcgctggcg atccgaatca gtatgttgtg gcagcagcga ccagcaatct
     8641 gattactctt gcagcgcccg gactgcgtca ggatctggcc gatgatactg caatcacagt
     8701 tgttggctcc tttactgcaa atatggcgtt tgatcgcaac gcgtttctgc tggcatcccg
     8761 tactccggca atgccggaag gtggcgataa tgctgatgat gtaatgaatg ttacggaccc
     8821 gatttcaggg attacgttcc agattgcact gtatcgtcag tatcgccagg tgcgctatga
     8881 agttggactg gcatggggtg tttcatcagt gaagccggca catggttgtc tgattcttgg
     8941 ttaaacattc aaacggggct tcggccccat ttttaatgga gggcatatgg ccggattaac
     9001 taaagagcaa agagcacagc gtgaagccgc acaaaaaacg caaattgagt tagtggttat
     9061 ggtgaccgat tatcagatgt ttcccggcgc accaactatc gctaatgttc atcctgatga
     9121 agttgacaac tggaaggcta tgggctggaa aactcaggag taacacatga tcacttacgt
     9181 gacctgtgat gacgttgata atgcgtttgg gaacggctgg acgagtgaga acgctaaaaa
     9241 taaagctgtt ttaatggcta atgcctggct taatggcttc aacctgaaaa ttaacccatc
     9301 ccgtattccg gaagaggtaa aacttgcggg agcatatgca gccagaattg cctcattcgg
     9361 taagttgttt cagcagaaaa atgattctgg cgttgttatc agtaaagccg tgtcggctga
     9421 cggggtcagt gtatcgaaat catttgctga gttgccagca aacagcactg cattgcttga
     9481 acccgattta cagcttgcga tagcactgct gaaaccgtac ggacttagtc gctcacaagt
     9541 cagggttgtg agggggggat aatggggctt cgtgaagaga ttcagtcaga ggttgccgct
     9601 gcttttgatg aggatttagc ggacgccgtg agtgattttt ctggttctta cgttacgcac
     9661 cggcactggg atcctgtgac ggaaactggt ggcgaatcca ccgcaatcta taccgggcga
     9721 ggcgtgttga cacgttataa gctgggcaga attgatggga ttaatatttt gcatggtgac
     9781 cttaaattaa ccgctctggt atgcgaggtt acggataaac ccgctgtcga ccatattatt
     9841 gagatttatg atcctgtatt aaggcagtta caacgatacg aggtaattac agcgagtgta
     9901 gatccttccg catcagttta ctctatccag ttaaggagag cgtaaatggc aaaggcatgg
     9961 gatatagaac cgtcaatatt tgccgggatg attgaggaag atgtagggct gaagattcgc
    10021 tacatcgcta tgcagattct tactgctatt gatattgctg ctccggttga taccggacgt
    10081 ttcagaaata acaacctggt gtcgttacag catcccgatt ttggtatatc tgataacgtg
    10141 gatccgaacg gtacgattgc tgttcagcgt gggatcgggg ttatttcgaa agctgcaaat
    10201 tacggggtta tttatatcca gaataacctt ccttatgcag aggctcttga aaacggtcat
    10261 tcacaacaag cgccaactgg cgtgtatgcc aacgcttttc atggtgtttt acaggcttac
    10321 aaatgacgtt tactgaaatc agaaatacgg ttatttccag aatgacggca cagacgatta
    10381 ttgatggaaa agacgtattg tatccgaacg ggccaacgtt cgatccttcc ggtaagttaa
    10441 tctgggcgcg gttaagtaat attcccgggc aggctggagt taatgaaatt ggcgcggggc
    10501 cggttgttta tcgcacgggg ataatcatta ttcagttatt tgtccccgca ggttctggtt
    10561 caaaactcat tactgagaca gccgataaat tgcgggaact gtttgagttt caggatgacg
    10621 atcgtctcag ttaccaggct gtttcctcaa tagccgtcgg cgaaaagaat ggctggttcc
    10681 agcttaatct tcaaattcca tatcgcgcgc tctagcgcaa ttaatgacat aggaggctcc
    10741 tgtgagttca ggtgcaaagg ttatctcggc atttatccgg gagacggttg caggcactac
    10801 gccagcaagt ggtgactgga gtttattaaa acgcacaagc tggggagtaa aacccaccca
    10861 gaataaaggc gaaaataatg agattggtgg ctcccggatg gctcaggggg cgacgcctgg
    10921 cactgtggat gtcggcggtg atgttggtac caaatttcgc tggggtcaac atgatgattt
    10981 tcttgcatcc tgtttcggcg cggaatggtc aggcgattct ctgacaatgg ggaatgagcg
    11041 aataacattt tctctggcga cctatgcgtc cgatgtcgga attgcctctg tcgtcagagg
    11101 agcgcaggtt ggctcatgga aaatgcagat ccctaacgac ggcgatatta cagcgaccgt
    11161 aacctttgcc ggactggact gggaatcaaa ggccgatgat acgaatttta tcaaaggcga
    11221 acctgtggat agtgcaggaa agctacgtta ttcgtttaag gaggtttcag cagtaagcct
    11281 gaatggtgtt gccggaggta acggtttttg tatcgacagt tttgatattc agttcgataa
    11341 caaactccag acacagcgtt gtatcgggac tggctcgcct tatgcaggag caaatattcc
    11401 gactactttt acaccgtccg gtacggtgac gctttcatgg tctaaagccg cgtgggaaat
    11461 ctggagtaaa acactgactg gagaaacagt tccgttcagc ttcacgcttt cgaatggaga
    11521 gggggcatac actttcagtt tcccgaaggt tcaggtgtca ggtgaatggc ctgatggggg
    11581 taatagcgac attatccagg ttcaattgag cattaccgca gcagatgaag cacctacgat
    11641 aaccagaaaa aaatgctccc caactgccgt gatcgcaaaa gccagtgctg atgcgattag
    11701 ttgattttcc gttattcccc ctgtggtact gcactacagg gggacgcatt gaatgaggtt
    11761 atggatgttt attcttaatc agaaaattat catcggtgga gaacgctggt ttacgccaat
    11821 gaaggactta aaacctgtag acgggttaaa actgttggtg gcaagcagcg ataacgatca
    11881 gtatcgctcc cgtaatgcat taatccgtcg tcacattgag aaaatggatg ccagtttgca
    11941 cgtcggaacg aaggagtttg atatttcaaa ggtttccgag gtagattctg ttgatgattt
    12001 actcattgat aatgctgctc gttatctgct gaaagactgg aaaggggttg gtgaactggt
    12061 taatggtgct gaggttgcac tggaatatac gccagaacga gggatcgcgc tgcttaagca
    12121 gaatccagag ttgtactggc agatccttgc agaagcagcc agcatcgccc agggtaaaga
    12181 gcagcagaag caggatacga taaaaaagcc atagccgccc agcggtggtt atcggagttc
    12241 gggggagaaa ggggggaaaa ggcaagatgg aagcgagaaa aactcaggtt gccaccgata
    12301 ccggaaccag aaatagaccc ggtgcttaag gaattgttgt acgcctattc ggtaatatcc
    12361 cgtgcccgac gttatgctgg aatgaccggg gtgcctttgc ctttatctct gacagagata
    12421 aatgaatatt tagccactca tccggtattg attgagcgcg atgagtttga agcagtgatc
    12481 tttgcactgg atgaccagta ttttcaggat cagtgtgtgt agttgttaat tacgtacact
    12541 ctgttacaga gatgtgatgg tgtctttaat taaatcgatg atgctcctgg agaaaagcat
    12601 tgcgtggcct cgtaatcgct atatctacta ttatgtcgcc tgaaacccac ttcggtgggc
    12661 tttttgttgt ctggagtttt aataaatggc agagcaaacc tcgcgtctcg caataattat
    12721 tgatagcact ggagcaaaaa ataatgctga caatctgacc tcctcattag tcaaaatgac
    12781 gcaggccggg gaaactgctg caaatagcgc agggaaagtg actaaggcaa cagaagatga
    12841 gaagaacgcg ctcgcaaaat taaaagcagc tattgatcca gttggtgccg caattgatac
    12901 tgtcggtcga cgctattctg aattaaagaa att
//
LOCUS       DAZUCE010000140        12867 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZUCE010000140 DAZUCE010000000
VERSION     DAZUCE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.140"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            92..175
                     /gene="idlP"
                     /locus_tag="ACHY03_003381"
     CDS             92..175
                     /gene="idlP"
                     /locus_tag="ACHY03_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFS3906573.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            172..564
                     /gene="iraD"
                     /locus_tag="ACHY03_003382"
     CDS             172..564
                     /gene="iraD"
                     /locus_tag="ACHY03_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFS3906574.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQLHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLALSLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(557..1468)
                     /gene="hypT"
                     /locus_tag="ACHY03_003383"
                     /gene_synonym="qseD"
     CDS             complement(557..1468)
                     /gene="hypT"
                     /locus_tag="ACHY03_003383"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFS3906575.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(1533..2705)
                     /gene="iadA"
                     /locus_tag="ACHY03_003384"
     CDS             complement(1533..2705)
                     /gene="iadA"
                     /locus_tag="ACHY03_003384"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS3906576.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            complement(2718..3179)
                     /gene="yjiG"
                     /locus_tag="ACHY03_003385"
     CDS             complement(2718..3179)
                     /gene="yjiG"
                     /locus_tag="ACHY03_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFS3906577.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            complement(3176..3859)
                     /gene="yjiH"
                     /locus_tag="ACHY03_003386"
     CDS             complement(3176..3859)
                     /gene="yjiH"
                     /locus_tag="ACHY03_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFS3906578.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            4109..4693
                     /gene="kptA"
                     /locus_tag="ACHY03_003387"
     CDS             4109..4693
                     /gene="kptA"
                     /locus_tag="ACHY03_003387"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFS3906579.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWANIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGGRHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            complement(4817..10057)
                     /gene="iatD"
                     /locus_tag="ACHY03_003388"
     CDS             complement(4817..10057)
                     /gene="iatD"
                     /locus_tag="ACHY03_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter IatD"
                     /protein_id="HFS3906580.1"
                     /translation="MASTNAHQIKNNDQNSLCGLGDKIRRLTAGVCLFTQFFFPVMAT
                     AQNVVHAKPQTTVSSAPPLLENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTSQKSHEQQNAIPPANGENMLENQIASTSQRVGSLLSQDM
                     NSEQASGMARGWASSEASGAMTDWLNNFGTAKISLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTGLGWRHFTSSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDNDFEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSMQKQLNSDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIRLSLLDPVKGKSGEI
                     KPLVSSLQTKYALKGYNIEAAALEAAGGKVSTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNLSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYTQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPVVSSRDHSSITIDNVSYYAGDDIKVRVELKDESNQPVAYQKEELVKAVT
                     VENSKPGTTIVWHEEQPGVYTANYPAHKQGTALRAQLSLHNWNAPLQSHIYNIEANQN
                     KARVATLSATNNDVYADKKTFNTLTINVTDESDNPLTNHQVTFKNEKGSAEFVEPPQQ
                     NTDGYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTAIVTDFHNNPLKDMKVNFVAPGGSQLDNTTATTDQSGIVRVHLTSSKA
                     GSYSVDASLEVDKNIHQSVTITVVPNREQSVMTLNARSGSAIANNTNIVTLTASVKDV
                     YGHPLPDEDVKFTLPASMTGNFTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVAYQQVSFIGDTNSAQLQPLTASLNSIVAGNSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSGTEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQASDNKTFNLSVLPDESS
                     AKVISITGAEKTITVGENITLRILVQDAFNNVIAGQRVRLSAQPTTNITIGDTAYTDN
                     NGYAYVNLLSTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNARGELMPGQIITFSVTPEGATLSNTGEVLTDQSGQAKVTLTSDKVNVYTVTA
                     IMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGIDSSTITSRVEDDYGFPVE
                     GVDVSHGLDTKGSPVVNIPTTRTDQSGQVTATITSTLAETLTVNVQVPGTANQSATIT
                     LVAGTADESKSILKSDVDTLKADYQQSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSISLNLIQSIKEMSG
                     HVTANNHTFSTAKFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQGWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYN
                     LPGITHLTSGENKRTQGSLYGEWGNVGAFSSNSQFTPGAYWTSESDDYSRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(10268..11446)
                     /gene="yjiJ"
                     /locus_tag="ACHY03_003389"
     CDS             complement(10268..11446)
                     /gene="yjiJ"
                     /locus_tag="ACHY03_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3906581.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GVFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            complement(11514..12356)
                     /locus_tag="ACHY03_003390"
     CDS             complement(11514..12356)
                     /locus_tag="ACHY03_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFS3906582.1"
                     /translation="MKKRITFIVFLCAIVAASLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSSQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKTLQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVRGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            complement(12626..>12867)
                     /locus_tag="ACHY03_003391"
     CDS             complement(12626..>12867)
                     /locus_tag="ACHY03_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFS3906583.1"
                     /translation="SIDKQANDSTVQQSIDSTVQQANDNIDKQVNDSTDKQTNNSTEQ
                     QDSNSFNQARLKEVVNIRFSIPGLTSYQPTYIVEE"
BASE COUNT         3162 a         3136 c         3243 g         3326 t
ORIGIN      
        1 tattcatagg caagtaaggt tttatacttt gctgacagga ttcaggcctg tctcagactg
       61 acatggatgt aatgaacaaa agggaatggc tatggaaaat gagcatcaat acagtggtgc
      121 ccggtgttca gggcaagccg catatgttgc taaacgtcag gagtgcgcaa aatgatgcgc
      181 caatcacttc aggctgtttt acctgaaatt tcaggcaata aaacgtcatc gctgcgtaaa
      241 tcggtatgca gcgatctcct cacacttttt aattctcctc attcggcatt accgtcatta
      301 cttgtttcag gcatgcccga gtggcaactg cataatcaga gcgacaaaca tctccagagc
      361 tggtattgcc gtcagctgcg ttctgcactg ttatttcatg agccacggat tgcggcgttg
      421 caggtcaatt tgaaagaagc gtactgccac acgctggcgc tcagccttga gataatgctg
      481 taccacgacg atgaaccgct aacgtttgat ctggtctggg ataatggcgg ttggcgcagt
      541 gcgacgcttg agaatgtcag ctaagcacaa tctccagctc gcgcagttca cgccagaagc
      601 gttcggcaac gggattcatg cgggtattca tccggtatgc gtaggcctga atcgggatca
      661 ccagttcgtc ccgattcagc acaacgagct gcccgctgcg aatttcttgt tggatggcgt
      721 attccggcag ccaggcaatc ccacagccgt cgagggcaac ctgctttaaa agctcgctca
      781 tcgaagagac aaaaaaggtg ctgaaactta actcactgtg gcgcgtcagg gtgcgattaa
      841 tcaatcgccc catgtaggag ttgcggctgt aattcagtaa cggaaagtgt ggctgcgcga
      901 ggttaaaaag tgcttctccg tgttcgtcac tggcgcaaac ggggaacaat tgagattcaa
      961 ataagcgaat gtggtcaaac ggcgcttcca gcaaatcttc gtcgtgaaag gaaaaaatac
     1021 agtcactttg cccttcacgc agtttatcga ctgcttcatc gacatcaatg gcttcaattg
     1081 cccaggtaaa gagcggcggc atctggctga taatggacgg taacagaccg agggaaagag
     1141 agtgtgcagc ggctatcttg attttacgtt gcgcgtaatc gctgccgcca cgcagctctg
     1201 caaggttact ttctaactgt tgcaacagat ggcggatctg cgaatggaag attttccctt
     1261 gttctgagag ttgcagcggc gtcacctggc ggttaaacaa ttcaacgcca atcgcctgtt
     1321 ccagtgcacg gatgcggcgg ctgaatgccg gttgcgagac gttgcgactg actgccgcct
     1381 gggaaaaatt gcggcatttt tccagagtca gaaaatcata aagccatttg gtttcaatat
     1441 tatgcaaaac cgcaccacag tcatccatta cgttttactc tgtcagccgc ccttgcgggc
     1501 attctacgtc cattagggcg gctgacaacc gtttaagccg tttcaaacgt tcctttcacg
     1561 caggctttgc cgtctttgac catcagtttg ccgcgagcat atacctgctc aatgcgcaat
     1621 tctggcgtca tgaccagtaa gtctgcgtca ttgcctggca gaatttcgcc tttcccgctc
     1681 aggttaagga aaccggctac gctactggtg agcgggcgca gggcatcgct gatactgaaa
     1741 tcatagtctt taaccagcac ctgcacggtt tccagcaacg tttcaaaacc tgcaacgccg
     1801 atatgggtta aattcccttc atcatcgaag aacggctggc taccgttgcc gtcggagctg
     1861 agggtgacgc gtgccagcgg aatacccgcc tgaacggcgc gggcaatacc ttcggcaggg
     1921 gcgaccggtt cgtcaatgct gctggtgata tcgatggtgc cgcctttgcg cgcgaactcc
     1981 agcgcctgct caaacaacgg tacgttgcgg ttaacgtggg tcggcagcag cttgctgatc
     2041 ggcacatcgc agttttccag caggtcatag ataggctgta acgccttttt actgtcgccc
     2101 atgtggaaca cggtgacgcc aggtttactg ccgagcaaac cgccaacgcg ggattctgcc
     2161 gccatattag ccaggtgata aacgtccggt gcggcagaac ggtgatcaga gatggcgcat
     2221 ttcacgccaa tcacacgatc gataatcgcc acgtcttttt ccacggaacc cgtaatagtg
     2281 cgggaaggga catgataagc gccggtcagc atccaggcgc tgatgccttc ttcattgagc
     2341 gcacgggtct tggcgagtag ggattccggg tggcgagaga tagagtcggt gcccagcaga
     2401 ccaaccactg acgtgacgcc cgcttccgtc aggcgactta gcgccacttc cggcgtgcgc
     2461 gtcgtgggac ctgcttcacc gccaccgcca atcaaatgga cgtgttgatc aataaaacct
     2521 gggcagagga tctgcccact gagatcgaca accgtgcagt tcggtacaat gtcagaaggg
     2581 atattgctgg caacggcgat aattttgccg ttagcgacga ggacatcgca aattccccga
     2641 tcttccggcg catacaaatg cgctccctgc agcagggtaa aaccggctgc ggtataatca
     2701 atcatggtaa ctccttgtta aacaataagc tgcataaccc agatcgacag caatgcgtta
     2761 atcacgcaga ccgtaatgat atgcgggtaa tatttggcat tcacttcggc tgtccccagg
     2821 cagcgaccga cattttgcac cgggttgccc attaaataca ttgcgggcag taaaacggtg
     2881 acatcgtggc cggttaatgc gccagcggtt gccagactgg cggcaacacc gactgcgccg
     2941 cccatactca ttaatgcggc taaaaggacg gttgccgctt caccgggtaa tccccacagc
     3001 gccattacgg gttcacagat atgccccacc cagtcgagca ggccggtaat tttcagcgcc
     3061 tgaatgatga caaacgccat caccacgttt ggcagcaggt tggtcgtggc gatagtaaag
     3121 ccacggcgcg cgccatcgat aaacatatcc atgacgtttt tgcggacctg agttgtcatg
     3181 cttgcgctcc ttgcgttggg ttgcgacgtt cttcgaagtt aagccagacg cgcaaaatgt
     3241 tggctccgac aaatttaaac accaggatga cggctaaagg tacgattacg gacgtgccca
     3301 gaaaggcaaa caccgcgacg ccggaagaga agtagttggt gatgatggcg ctaccgctgg
     3361 tttgatatgc ggcgaaaatc actttatcgc gttcggtaat ttcaccttcc tgcgccagct
     3421 ctttggtcat accggcggcg gcatcagtgt tttgcaagtt agcgatcagc gccagcgagc
     3481 agatccctgg aatgcccaac aacggtttta gcacaggtgt cattaactgc tgcgcggcgc
     3541 gtaatccacc aagaccatcg gtaattgaaa taatccccag agagagaatg actgacggtg
     3601 ccagctccag cgcaaagaga aagccatctt tagcccccgc accacccgcg ccgcgaaatg
     3661 aggtggtggc tccgttcgca ccaggcagtt gcccaaacga tccgttcaga acggaaaaat
     3721 caaaaacgcg ccaccagctg tctgttccgg aaaaaacacc ggaaaaaaaa attatggtga
     3781 gaaaaaaagc cagataccct ttaatgccca ctttttctgt cgccagctcc ccggctacag
     3841 catccccttg ttgtgtcata acaatcccca ttgttttttt gtgtttgtgt gttgcttgag
     3901 ccaggaaaat tcctggaact gaaaaacctt atcagccatg tgggtatggg gaaaatgcaa
     3961 gtatgtttct ggctatgcgt tttttgcata ggggaaacat tacgctgtgt ggaaaaggtt
     4021 ctgcgcagga ttgagttgca gaaaattcat aaacccagtg acaatgacat tggtaaataa
     4081 aaacagaaaa cccagggacg aaaacgcgat ggcaaagtac aacgaaaaag agctggcgga
     4141 tacgagcaag tttttaagtt ttgtcctccg acacaaaccg gaggcgattg gtatcgtact
     4201 ggaccgtgaa ggatgggcga atatcgataa gctcatactt tgtgcccaaa aagcgggcaa
     4261 gcgattaacc cgtgcattgc tggataccgt ggtcgcaacc agcgataaaa agcgttttag
     4321 ttattccagt gatggtaggt gcatccgcgc ggttcagggg cattcgactt cgcaggtcgc
     4381 tatttccttt gccgaaaaaa caccgccaca gtttctctac cacggtacgg caagccgttt
     4441 ccttgacgag ataaaaaaac aggggctgat tgcagggggg cgtcattacg tgcatctctc
     4501 tgctgatgaa gcaacggcac gcaaagtggg agcaagacac ggatcgccgg ttatcttaac
     4561 cgtcaaagca caggagatgg cgaaacgagg catccctttc tggcaagcgg aaaacggggt
     4621 ctggctgaca tcaacggttg ctgttgagtt tcttgagtgg tcgtttatgc cgactggggt
     4681 acaaaaacaa taaattcaat atattgcagg ggctatatag gcctgataag cgcagcgcat
     4741 caggcaatgt agcgtttgtt atcagtctga aaggataccc tcgcaaaagg gtatccttta
     4801 ataacaactg ctgaaattaa agtgatttac ggcaggcggt cagttggggg ctggaatcag
     4861 cgtcgcttcc ggtcataccg gttagcatct gcacatagta gtgccgactg taatcatcag
     4921 attcacttgt ccagtaagct cccggtgtaa attgcgaatt actggaaaac gctccaacgt
     4981 tcccccattc accatacagt gatccctgcg tgcgtttgtt ttcgccagac gtcaaatgtg
     5041 tgatgccagg aagattatat ccatcatttt tagcatgaca gagcgcattg gcagctagcc
     5101 agatattggt atgatttcca ttattcaccc accagccttt cagcttaatt aaggtctggt
     5161 aggttgtacc tccttgtcgg ggaacagcgc ttattgtgac tgacgtttga tcgccgatat
     5221 ttgcgaaaga aactttacct gaagcatcga cagacaccca accctgtgaa cttgaaaaca
     5281 tataatcatc aacggtttta cccgcttcaa agttatcatt gttgagtgtg taatacgccc
     5341 ctgcaaagcc ttcgctcggg aatttagccg tggagaaggt atggttgttt gcagtgacat
     5401 gaccggacat ttcttttatc gattggatga gattcagcga tatgcttaag tttgcctgat
     5461 gaaccccgtt cagcatggga atgagggtcg ctgtcccctc ttttccgcca gtgacagtca
     5521 cggtgtactc gccataattt ctttggctgt aatcaatatc gctcaacttg agaaagttca
     5581 cgaaggggcc tgactggaca aattccagat gatcagacgt cactatcggg ttaccgtact
     5641 tgtcttgcaa tgttagcgta agttttgcgc tctgctggta gtcagccttc agagtgtcaa
     5701 catcggattt caaaattgac ttactttcat cggccgtgcc ggcaaccaat gtaatggttg
     5761 cggattggtt ggctgtacca ggaacttgca cattgactgt taaggtttct gccaatgtac
     5821 tggttattgt cgccgtgact tgcccggact gatcggtacg cgtagttgga atattaacta
     5881 ccgggctgcc tttggtgtct aagccatgac taacatcgac accttcaacc gggaatccgt
     5941 aatcatcttc tactcgtgaa gtgatggtgc tgctatcgat gccgtcggcg gtgatggtgt
     6001 caggagaagc cacgacgctc acaacatgtg ccgttttagc atctgcctta accgcaaccg
     6061 ttacctggct ctgaacggga acatctttgc ccattatggc cgtaacggta tagacattca
     6121 ctttgtcact ggtcagcgtc actttggcct gacctgactg gtcagtaagg acttcccctg
     6181 tattgcttag cgttgcacct tcaggcgtta cgctaaaggt gataatttgc cctggcatca
     6241 attcaccccg cgcatttctc gccgttgcgg taagcgtaat accctcacta ttatggaccg
     6301 tggtttctgg tttcgatgat gttaactcga gtttgccatt tgccacattc acgtcaacct
     6361 tactactact gttattgtcc agcgttgccg tcacctgata aaccccaggt tgggtgctga
     6421 gaaggttaac gtacgcataa ccgttattat cggtgtaagc cgtatcgcct atcgtaatgt
     6481 tagttgttgg ctgcgcactt aatctgacgc gttgacccgc gattacattg ttaaacgcgt
     6541 cctggacgag tatccgtagc gtgatgtttt cgcccaccgt tatcgttttt tcggctccgg
     6601 ttatacttat taccttcgcc gaactttcat ccggcagcac tgataaatta aacgttttat
     6661 tatcggaggc ttgcgctttc cggctcacca cgacgttatg ttgtccggca atattgctgg
     6721 tcattgttac cgtcgcctga cccagcgtat tggtggtgac ttctgttccg cttagagtga
     6781 catcgttact ctggaaagtg accaactggt ctttaagcgg attttggtaa gcgtccagga
     6841 tcgttgccgt cagggtactc cccgtactgt tacccgcaac aatggaattt aatgaggcag
     6901 tcagcggctg gagctgcgca ctgtttgtat ccccaataaa agagacttgc tgataagcaa
     6961 cggtattatt tctggtcagc gtcgccgtaa ctgtaaactc acccgctttt gtgcctcgca
     7021 atgtgaccac ggcatcaccg tttgcatcgg tgcgggcggt ttcactactt agcgtgaagt
     7081 tcccggtcat ggaggctggc aaggtaaatt tcacatcctc atccggcaac gggtgtccat
     7141 aaacatcttt cacactggca gtcagggtaa cgatatttgt attgttagcg atcgcactgc
     7201 ccgacctggc attcaaggtc attaccgatt gttccctgtt tgggaccacg gtgatcgtga
     7261 ccgactggtg aatattttta tccacctcaa gcgaggcatc gacggaatag ctaccagctt
     7321 ttgaactggt caggtgcacc cgcacaatac cggactggtc tgttgtggcg gtcgtgttgt
     7381 ccagttgcga gccaccaggt gccacaaaat tcactttcat atcttttaac gggttgttat
     7441 gaaagtctgt gacgatggcg gtcagagtac tgccctggct gttaccagca ataatggtat
     7501 ctggatcggc aaccagttgt ttgaattttg gcgtactcga atcgctaacg aatttcgcaa
     7561 ttatccgttg tgagaaacca tttggcagcg tggcgctaat cgtattttct tccgcaacct
     7621 gacttaccat gttgattgtg gcaacaccgt atccatccgt attttgctgc ggcggttcga
     7681 caaattccgc gcttcctttt tcattcttaa aggtgacctg atgatttgtc aggggattat
     7741 cactctcatc agtgacgttg atagtgaggg tattaaatgt ctttttatca gcgtaaacgt
     7801 cattattcgt cgctgataat gtggcgacgc gagccttatt ctggtttgcc tcaatgttat
     7861 aaatatgcga ttgcagtgga gcattccagt tgtgaaggct aagttgtgcc cttagtgcag
     7921 tcccttgctt atgggccgga taattcgcgg tataaacgcc cggctgctct tcgtgccaga
     7981 caatcgtggt gccaggtttg ctgttttcga cagtaacggc ttttaccaat tcctcttttt
     8041 gatatgcaac cggttgatta ctttcgtctt tcagttccac cctaaccttg atgtcgtctc
     8101 cggcataata cgatacgtta tcaattgtta ttgatgaatg gtcgcgggat gagacaacag
     8161 ggacgatatt aacgacgatg gattctttta ccgcactctc accgttaatt tgcggcgtca
     8221 gtgttactga acctgatgtc gttccggcgg tcagcatctg tgtgtaacta ccatctccgt
     8281 tatctgtcca gtcagacagg gtgatatcct gaacgccttc actgcgggtt tgcagcgcca
     8341 gccccggcac cggagttccg tcggaatcgt gtgcagtcac ggtcaatgtg gtagtcgatt
     8401 ttttatctgc agccacggtt agcggattaa cggataacag agaatctttc tggcttaatg
     8461 tcggagcctg aatgacgacc atgctttgtt catgacgtga caggttacct tttacatcct
     8521 cggcggtaac gtctatcgac catgtattat cggtttctgg ggtgttagtg aagcggtaac
     8581 ctggcagcgt gaccgtgata tcttttccag acgtgctgac tttacctccg gcagcttcca
     8641 gcgcagcggc ttcgatgtta tagcctttca gggcatattt ggtctgtagc gaggaaacca
     8701 gcggttttat ttctccagac ttccctttca ctggatccag cagactcagg cgaatcagct
     8761 ctttcttgcg gtattccaga acgatgttgt tgttgcgatc aatcaggtca taacgactac
     8821 cggccagact gcgacgtccg gccacttcgt ccgagttaag ctgtttctgc attgaactgc
     8881 tgggttgcca ggtcagatca acggcaaaac gtgtgtcatt ttcaccttgc ttcccctgac
     8941 gctgttccgc actgagagtc agaagcggga agggggtata gttgaggccc gcggtaatag
     9001 catggggatt actttgacga tcattcttgt caaacagtgc cacttcatcg ccatagtatt
     9061 gttcatagac cagttttccc cccagttgtg gccaggcagg taaccagcct tccgcgcgta
     9121 aatcccagcc gttggccggg cgggcttcga agtcgttatc caattctggt gcgctacgcc
     9181 agccggtcag gccgatataa gcgttgctgc tcaacttcag ataatcacgc cagtattctg
     9241 cgccaagccc tgcgcgggag tgatagcggc tcaggtcgtg gtcaaaaaac aggttgatgc
     9301 ctgacatcca gctggaggtg aaatgacgcc agcccaaacc ggtgttgatc tgggtacgat
     9361 cgtctgttcg gtgaagggta tgctggctga agagcagata atcgggtgtg tcataccacg
     9421 gatgcaggaa gtcgaattgc gaatttttca ggctgaaatc ctcatccaca cccagagaga
     9481 ttttcgcagt gccaaagtta tttaaccaat cagtcatcgc gcctgaggct tctgaagacg
     9541 cccaaccacg cgccatgccg ctggcctgct cactattcat atcttgtgaa agcagagagc
     9601 caactcgctg cgaggtgctg gctatttgat tctccagcat gttttcgcca ttcgcaggcg
     9661 gtatggcatt ttgttgctca tgacttttct gcgaggttgt tgccggaaca tccagttcct
     9721 caccctggcg cacgttatca aagccgcgag caaaagtacg gaactgatta agacgacgaa
     9781 gctcctccag tgaaataccg aatcgatcag caacgctttg cgctgattcc agcgcaccaa
     9841 gcgtgtaagg caccgtatta ttttctagta gagggggggc agatgaaact gtcgtctgag
     9901 gtttagcgtg taccacattt tgtgccgtcg ccatgacggg gaaaaaaaat tgtgtaaata
     9961 aacaaactcc ggcggtcagg cggcgtattt tgtcacccag accacataaa gagttttgat
    10021 cattattttt tatttgatgc gcatttgtcg aagccatagt gaccttccgt gaaaaaacag
    10081 aattaaactt gttttttcta gcacggagat ataatgcgat cctgactata atatgaccag
    10141 aaataatgaa ttagccgtgg aattgctaaa ccaggaataa taccggcggt ggctgagtac
    10201 ctccaaagaa aaaggatgtt ttaattattt tacgtgatgt actgaaaagt taaattcgtt
    10261 aaattactta agaataattc gtatgcaatt tgaatatttg catcccgcag attaatgctg
    10321 cgataaatag cgccgctgcg ccgcagagtg tggcgctggc gagcgccgac gttccgttgc
    10381 cgagcatact ggtcagcgcc gggccaagaa tttgcccgat accataaatg agtgtcacaa
    10441 agcccaaaag gttaagattt cccggcacgc taagctggcg ggcgatggtc atcaccagcg
    10501 aggtcgttcc cataaaggtg ccgccaaaac caatactgct gatgatgagt aataaaggag
    10561 agctgctggc gagggttaac agcacgcaga tcgcctgcac cagcaaattc gcggtcaggc
    10621 aaggtaatgc tccccaccgt ttggctgccc acagccagcc aaagcaacct gggacaatcg
    10681 acaacccgac cagtgtccag aggtgagccg tcagcacagg ctggcccgcg tctttcgcca
    10741 tgagcggcag gtaggtggcg acgatgatat aaccaaaacc cgccagacca tacagaatcg
    10801 ccagtaacca ccagctcatg ggttgttgcg cgatttttgc caatggcgct ggcgggataa
    10861 cgtgtttatt cgacgggatg agcagggcca gagcaagcaa tataatggca gaaagtgctc
    10921 cggcaccttg ccacaacgtt tgtgaagaga gggcaaaatg caggcctgcc agcacatatt
    10981 cattacccag agcgatgccg acgccaacac cagaaaatag cgccgcaagg acaaagggat
    11041 ggcgagtatg ttgcatgatg agtgttgagc cgaaaatcaa catcccggcg ctggcgactc
    11101 ccgccagaaa gcgaatgatg aaaaccagaa gaaacggcgg cagccacgcc atcgcgagga
    11161 ttaataatcc ggttgcgagg gcggaagcta acaggaacgg gcgcaggcgt gagggtaaat
    11221 gaaacgcgcc gaatgaaaac agcaggctcc ccgccagata cccggcatag ttaccgctgg
    11281 cgatccatga gagttcgcta aaggaaaaca cgccttccgc cagcatgact ggcagcatcg
    11341 gcgtatagag aaagcggcct aaacccatac ctaaggtcag aaccagcatc ccgaataacg
    11401 cggtgctgaa agaaaaacgt tctacgggat gcgatgtcga attcatgttc tgcctggttt
    11461 gtattatttt gagttaacaa gttaataaca aaaaaggaga acctgccaga ctgttatcgt
    11521 gattttgtcg acgtaaagcg ataaaaaaga ttcggttcgc caacgatata gatgttgccg
    11581 ctatcatcca tcgctatccc ttccgcctgc ttgatgttgt gtgaaaggcc atacttacct
    11641 ttggttaacg acatttcgcc gatcacgtca cctctcacgg tcacttcctg cagggcgcgt
    11701 gactcatgtg acaacaccag caacgtattt ttctgtgggt taaattccgc gccagaaaca
    11761 tcgtctaaag taaactgacg ctgcaacgtt ttatctttgc tgatatgcaa ctcatcgcta
    11821 cgcaatagcc cggttacttt gtagacctct atcggatttt tctctttaaa aaaccagaag
    11881 gtatggtcct gagaggagta agccagccct tcaaagccac agttagttgg tgtttcctgc
    11941 aatgggattt taattttttt gaggatgctc acttccgagt cagcgttaag tgaaatcaca
    12001 taaatggcgt agtcacgttc atcactgatg acaaatttat tatcaccaat gtattcaatc
    12061 gtctcgagat ccttaacaaa atcgagcgga atggtgcgga tcaagtcgcc ctcggtcgtc
    12121 atttcaacga tggttgcagg tttgttgata gtgctaaata aggtattgct ctgcgctgac
    12181 caagtcagtg aggagatatt tttagttact cctgtaatct ctttaccatc gatagtggcc
    12241 tgataattct gaaaccctgc gacatgctgg cttttacgga cgcaggattg cacaaaaaac
    12301 aggcttgccg ccacgatggc gcaaagaaaa acgataaagg tgatgcgctt tttcaactta
    12361 ttgctcctta ttattgcttt ttttttctcg tcaaattcag tgcgacagat aaggagtata
    12421 cggatgaagc ttaatgaatt cttaacgcgg gcgatgaaat agtaaacgcc gcaggggggc
    12481 ggcggttgtc tctggatggc agaacatttt cagcttacat cgactgataa atgcgcctca
    12541 tccatgaatg atagatgagg cggttaacgt ttaaatgtta atctatatcg tctgtcatac
    12601 ccactctgtt tgggtgatta ttgaactatt cttcaactat atatgttggc tgataagacg
    12661 ttaaacccgg aatggaaaat cttatattta caacttcttt taaacgggct tgattaaaac
    12721 tgttactgtc ctgttgctcg gtactgttat tggtttgttt atcggtactg tcattgacct
    12781 gtttatcgat attgtcattg gcttgttgca ccgtactgtc aatagattgt tgcaccgtac
    12841 tgtcattggc ttgtttatcg atactgt
//
LOCUS       DAZUCE010000141        12774 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZUCE010000141 DAZUCE010000000
VERSION     DAZUCE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.141"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(81..1127)
                     /gene="fbpC"
                     /locus_tag="ACHY03_003392"
     CDS             complement(81..1127)
                     /gene="fbpC"
                     /locus_tag="ACHY03_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HFS3906584.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADTA"
     gene            complement(1139..3217)
                     /locus_tag="ACHY03_003393"
     CDS             complement(1139..3217)
                     /locus_tag="ACHY03_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3906585.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFVLLPSWSLDYGLLESTS
                     DEILGAYGWSHFNISWLWYLLPSLLLVRPFSEATREQRSRHYFDAGWALLCMAFIVIS
                     ATVVGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(3286..4317)
                     /locus_tag="ACHY03_003394"
     CDS             complement(3286..4317)
                     /locus_tag="ACHY03_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS3906586.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(4314..5630)
                     /gene="uhpC"
                     /locus_tag="ACHY03_003395"
     CDS             complement(4314..5630)
                     /gene="uhpC"
                     /locus_tag="ACHY03_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFS3906587.1"
                     /translation="METTMHARSAREIDQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLIIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(5703..7244)
                     /locus_tag="ACHY03_003396"
     CDS             complement(5703..7244)
                     /locus_tag="ACHY03_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFS3906588.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPALLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQIVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(7244..7873)
                     /locus_tag="ACHY03_003397"
     CDS             complement(7244..7873)
                     /locus_tag="ACHY03_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS3906589.1"
                     /translation="MIRVVLVDDHVVVRSGFAQLLSLEDDLEVIGQYSSAAQAWSALI
                     RDDVNVAVIDIAMPDENGLSLLKRLRAQKPQFRAIILSIYDAPTFVQSALDAGASGYL
                     TKRCGPEELVQAVRSVGLGGHYLCADAIRALRGGGQPAQALEILTPREREVFDLLVKG
                     DSVKEIAFKLELSHKTVHVHRANVLGKLNCHSTIELVHFALDHHLLAGH"
     gene            8176..9138
                     /gene="tauA"
                     /locus_tag="ACHY03_003398"
     CDS             8176..9138
                     /gene="tauA"
                     /locus_tag="ACHY03_003398"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3906590.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            9151..9918
                     /gene="tauB"
                     /locus_tag="ACHY03_003399"
     CDS             9151..9918
                     /gene="tauB"
                     /locus_tag="ACHY03_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFS3906591.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            9915..10742
                     /gene="tauC"
                     /locus_tag="ACHY03_003400"
     CDS             9915..10742
                     /gene="tauC"
                     /locus_tag="ACHY03_003400"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFS3906592.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            10739..11590
                     /gene="tauD"
                     /locus_tag="ACHY03_003401"
     CDS             10739..11590
                     /gene="tauD"
                     /locus_tag="ACHY03_003401"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFS3906593.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPLQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(11696..12670)
                     /gene="hemB"
                     /locus_tag="ACHY03_003402"
     CDS             complement(11696..12670)
                     /gene="hemB"
                     /locus_tag="ACHY03_003402"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFS3906594.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         3064 a         3455 c         3497 g         2758 t
ORIGIN      
        1 acactggaga ataatgttgt ccgaagccta accaggtgaa cggtccctca agacagatgg
       61 ggaccgttta tgtgctattt ttatgccgta tccgctaaaa caaacatccc gtaaggatgg
      121 atttcgaggt aatagtgctc accaatatcg ggctgtaaac gggtggcgtt tacttgtaat
      181 aaaatctcct gtccatgcca ctctacggtc acttcgtact gcggccccat gtaggcgaca
      241 tggcgaataa cacaacgctg actctcttcg ccgtgattgc ttaaggtgat ggcttccggg
      301 cgaacgccga cggtgccgct accttgcgcc gggaaatgcg ccgcgcgcgg caggcgataa
      361 ccgtagatat cgacatactc ttcgctaaag ttcgccggga acaggttggc gtcgcccata
      421 aaacttgcca taaatcggga ggctggctga cgatagagat cctgcggtga gccaatctgc
      481 atgatgtgac ctttattcat caccagcaca gtgtcggaca ccgcgaaagc ttcactttga
      541 tcgtgagtga cgtagagcga cgtgatatta aactgctttt gcagctcgcg gatcttatcg
      601 cgcatactgc ggcgcaggtt ggcatcgagg ttacttaacg gctcatcaaa cagcagcacc
      661 tttggcttga ggatcaacgc gcgggccagt gccacacgct gttgctgacc accggaaatc
      721 tgatcgacat agcggtcctc gaagccttcc agatccacca ttgccagcgc ctccttcacc
      781 cgctgtttca cttcgctacg cgagacgcca agcatcttca gcccgtagcc aacgttttca
      841 cccagcgaca tatgcgggaa cagagcgtat gactgaaaca ccatgcagat atcgcgctgt
      901 tgaatggaac gatgcgtgac atcttcgcca tcaataaaaa tttgcccttc gctcggtttt
      961 tccagcccgg caaccagacg caaaatggtg gttttgccac agccggaagg accgagcagc
     1021 gtcaccattt gcccttgtgg aatggtgaga ttgatattgt cgataaccgt attactgccg
     1081 aatcgtttag tgacgttgcg cagttcaaca aaatttttct gactcatagt gcgctccatt
     1141 acgcctggtt tttggcttta gaacgggaga tacgagcttc tccgatcagc cagtcaaaga
     1201 ggaaaataat cgccagcatc accacgatca gaatcgaacc ataggcgatg gctacgccat
     1261 attcgccgtc ttcaacgcgg ttaagaatgt aggcggttgc cacgcgggtg tccggcgtga
     1321 cgagaaagac gatggcgctg accgtcgtga tggcccgcac aaagctgtag atcagggccg
     1381 agagaatggc cggacgcagc agcggcagta aaatgtgggt tatggttcgc aaggaacccg
     1441 cgcgcaggct gagtgaggct tcatccagcg atttgtcgat ctgccccaga cccgcaatcc
     1501 cggcgcgaat acccaccgga acgttacgca tcaccatcga gataatcaca attgccgccg
     1561 ttccggtgag ataaaccgga gcgctgttaa aggcaagaat atacgaaacg cccgccaccg
     1621 tacccggtac ggcaaagcac agcatggtgg tgaactcgat ggtctttttg cctttgaatt
     1681 gttgacgcac caccacccag gcgatgagca ggccaaagat agcggtaatg ggagcggcaa
     1741 tcccggcgta gagcagggta tccagcagtg aaggccacgc gccgtcgctc attccctgac
     1801 caaatagctt gataaagtta tccagcgtca gggtgtagtc cacgccccag ttcaccgtga
     1861 agctgccgta gaaaatgctg ccgtagagca gggcgttaaa ggcaatccat atcgcaagta
     1921 tggcgaccac gctccacacc agggtgaccg gtagcggctg gacgtcgcca cggtaggatt
     1981 tgccggaaac cgtgacgtaa gagcgtttac caatccacat atactggatg cagaacacca
     2041 gcagcgagaa cagcagcagg aaagcgccaa gcgtgctggc ggcctgataa tccagctgcg
     2101 agccggtaat atagaagtaa atttgcgtcg ccagtacgtc gaagttaccg ccaagtacca
     2161 gcgggttact gaaatcagcc agtgactgta ccacgacgat taaaaaggca ttggcgagcg
     2221 cgggcttcaa caacggcaca aacacgccgt taaaggtttg ccagcggctg gcgcgcaggg
     2281 tgtaggaggc ctcttccagc gagggatgaa tggttttaat ggccccgtcg aggatcataa
     2341 aagccattgg cgtgaatgcc agaacctgtg ccagccagat cccggtaaaa ccgtacagcc
     2401 agttggtgtt ggtcagccca aaccactcca ccataaattc cgtaacatag ccggagcgcc
     2461 ccatcatgag cgtcacgcct aagccgacca caaacggtgg cgtaacgatg ggcaaaatgg
     2521 aaaagatgcg accaataatg gcgctgcgtt tagcgatgcg cgtggtataa atcgccagca
     2581 ccaggccgaa gaaagtacag cctatcccca ccgctatcga caagacgata gagttgacga
     2641 tcacctgaac aatatgtgcc tgcgagagca cggtcataaa cgccagcggg gcgaactctc
     2701 ccgcgtcgtt ggtgaacatc gggataaaaa tggcgatgct cggccagacg ataaaaatgc
     2761 caatcagcgc cacaatcgca atcagtgagc cgatcacgaa gcggtcgcca cccagccatt
     2821 cgaggcgggt taaggccagc gtcataatcg cgcctagttc gacaaacagc acgatggcgg
     2881 cgtagcctaa accgcgtcca accacggtgg cgctgatgac gataaacgcc atacagagca
     2941 atgcccagcc ggcatcgaaa taatgacggc tacgttgttc gcgcgtggcc tcactaaacg
     3001 ggcggaccag caacaggctc gggagtaaat accacagcca gctgatatta aaatgcgacc
     3061 agccgtacgc cccgaggatc tcatcactcg tggactccag caggccataa tcgaggctcc
     3121 agctcggcaa caggacaaat gccagccaac ccagcaaaac ccaaaggaaa atcgcatccc
     3181 gctttttcac gggatggagt gcaagtgtct gtgacatagc agttccggta tggttggccg
     3241 gggcaggcgt ctgcgctgca cccggcaaat gcgcctaatg cgggtttatt tacccatttt
     3301 tacttcgcta acccatttat taatcaatgc cttacgaaca tccgtggaac cgtatttgtc
     3361 catgtcgtag ttgattaatt tcaggtcgtc gagcttcagt gagtttggcg acgtttcggc
     3421 ggtggtattg gtcaggatct gataagactt ccctttcttc cacgccagtt cctgcgcttc
     3481 ttttgataat acccagtcca caaacagttt ggcgttgtcg aggttgcgcg cgcctttcag
     3541 aatgctgacg ccgccaattt cgtagcctgt accttcacac ggggaaatca actccagcgg
     3601 cgcgccctgt tctttttcca gcgagtagtc atgcaggaag ccaataccaa tcgccgtttc
     3661 gccacgggcg gcgttacgtg ccggggctat gcctgatttg gtgtactgag agacgttggc
     3721 gttcagttgt tttagataat caaaggcctg atcttccccc cacagctgcg caaaagtagc
     3781 cagggcggtg taggcagtac cggaactttg cggatcggca atttgaattt cgcctttata
     3841 ttccggtttg gtgagatctt tccagcactt cggcaccggc aggtttttct ctttcagacg
     3901 atcggtgttt acgccaaaac cgaggatccc cacataaacg gcagaggaca gattcccttt
     3961 cacttttgcc ggatcgcgga atttctccat gatctgctca aggttagggg atttatacgc
     4021 ctgtagcagg cccatctcgc cagcctgcga ttgcgggtct aaagtgccgc cgtaccagac
     4081 gtcagcctgg gggtttttct tttcagcatc gacttttgcc agggtgcttc cggaaccgtt
     4141 gcggataaac gaggttttta catcatattt ctcgccaaac gctttggttt cggcctcaca
     4201 catttcattg gtggcgctgc aatagaccac cagccggcct tttgcctgcg cggcacccgt
     4261 taaagtcgcc agcgcaacgg cagaagcaat cagagtagag gtaaaggtca ttttcattgt
     4321 tttatccttc gagcgtgagt ggtggtgatg cccgccatca gtaacggcat cagcagtaaa
     4381 cccataagta ctgcggcgac cgagagtaaa ctgaacattc ccggccagcc gtagcgttcg
     4441 atgatcagcg acagcggcca gcctgccagc gccgcgccaa ggtaagcgaa caggccgaga
     4501 aatccggtga ttgaacccgc cgcagctttg tgcccacact ccaccgccgc aagtccaatc
     4561 agcatttgtg ggccaaagac gaaaaagccc acggtaaaaa agcacactgc cagcagagcg
     4621 tagtgatgaa ccggtgccag ccacagtgcg gcgactgaca ccatcaaccc caacgtgaac
     4681 agcaaaatca ttggcgctcg ttgcccgcta aacagcaaat ccgatcccca tccggcaaat
     4741 aacgcgccga gtaagccgcc cacttcaaac agcattaccg tggcgttggc gctgagcagg
     4801 ttgacaccgt ggctttccgt cagccagata ttgccccagt cgttaagcgc aatccggatc
     4861 acataaacca gtacataaga gacgcccagt agccagatca gcggattttg cagcatggtg
     4921 gtgcgcaaca tttgccacag ccccatcggc ggactttgtt gttcctgacg taactccagc
     4981 ggatcgtgac gccagtgacc aactgtcggt aatccctctt cctggggcgt gcctttgagt
     5041 tgtagcgtta accagatccc taatgccatg ctgattatcc ccggtgtcag catcgcggcc
     5101 tgccagcccc accagtgggc ggcaaaggcg ctaatcagcg gaataatcgc tccgccaatg
     5161 ttgatcgaca tattccagca gccccaccag aaaccgcgct cattgcgtga gtaccagtgg
     5221 gtcagcaagc gggcacacgg tggccatccc cagccctgaa aaaaaccgtt cagtgtccag
     5281 accacaagca gaagcgtaag tgattcgcca aaggcaaaca ccacgttcaa taacccggtg
     5341 gcgaacaagc cgacgcccat aaaaccgcgt tgtccgtggc tgtcatgcca cagtccggcg
     5401 gtaaatttcg acaatccgta gctgagataa aacagcgaac ccagcaggcc aatgtcgcct
     5461 ttatccagtc cgaggtcggt ttgtagcgcc ggaaggacat aattcacgct ttttcgcgtc
     5521 agataaaatg cggcgtagcc aataatcatg cacatcagca atcgcggacg taatgtccgg
     5581 tagcattgat caatttctct cgctgaacgc gcgtgcatgg ttgtctccat gttgctgact
     5641 atatgaaaat gaaagtgaaa ggggatgaga gaaaatccgg gatacctaag actttttcct
     5701 ggttatccgg cgatttgttg caaaaatgtg ggcaagttaa caattacccg cgtgccgtgc
     5761 tgtttttcga gcgttaagtc gccgcccagg gcgctgacgc gctcacgcat cccctgaatg
     5821 ccaaatccgg ccattttccc gctggcgatc cccacaccgt tgtcgctgac ttccagatgc
     5881 aatacctctc cctgctggcg caggataatc gtcacttcgc tggcttcggc gtgtttacag
     5941 atgttattga gcaactcctg caataaccga tacaaggtaa agcgcaccgt ttcgttttct
     6001 ggtgtggagt tcagttgata ggcaaactgg caatgaatgc cgcgttcact aaaggcaaat
     6061 tcattaatca gatgcagcaa cgcttcgcga aatgtcagtt catcaagcgc gggaggccgc
     6121 aactggcgca gcaattggcg ggtggagaga tgaatgcggc gggcgagctc attgatttgg
     6181 ctggctgcgg attgaatttg cgccggatca cgcgcccgct tcaccagttg cgactggatc
     6241 tgaatggcgg tgatgttctg accgatttcg tcgtgcagct ctcgcgccag gttttttcgc
     6301 gtgtcctcct cggtgtggat cagcttttcc gccaatgccc gccgcgctgc cagctcggcc
     6361 tccagccgtt gtcggtaatg gtgcaggttt agcgccagat gttgctggcg actgatggca
     6421 atgcccaggc caattcccag caaggcctgg gtcgcgagga aaatctccag ctccaccagg
     6481 ttactaaagc ccacgccaat ctggcgggcg atggtgatca tcatgctgcc caaaagcccg
     6541 gagagtacgc caccttgcca gccaaatttc cacgccatca ccacattggg caaaaagacc
     6601 acgatcaaca gcagacgttc aatttcaggt gacagcacta tctgagtgcc gataccaata
     6661 ataaaaaaca ggctacacca gatgattaaa gaggtacgca aggggggatt atgcgtatcg
     6721 agtcccagga gctggtagcg atgctgctgg cgcagaaact caaagataag atagacaaac
     6781 ggcgtcagta atacgccgcc ggtaaatgag gtcaaacccg gcagcattat tgggcctgac
     6841 ataaaagggg agagcagggc ggtattcaac aatgaagctg ccgtgacagt ggctaacagc
     6901 aacgtcagac gctgccagta aagtggaaaa cggtgccaga agttttgcgc aaggagggca
     6961 ggaaacaggc taataaccgg cgttgccagc atgagataac cgttcaatag ctgttcacta
     7021 tggagccaga acatcatcag caaagggggg agcaccaacg caggccagta gcggcgagag
     7081 agcaaaataa caagggcgag ataaactccc tgcgggagaa acaacaccgc ctgttgaccg
     7141 ttattggtca gataaaagct gagcgtccac agcatcagcc agccggttcc ccaggccagc
     7201 acaataaaca gcgaaatcac ccaatgacga agattgcgcg acattaatgc cccgccagca
     7261 gatggtggtc gagcgcaaaa tgtaccagtt caatggtgct atggcagttc agttttccca
     7321 gcacattggc gcgatgcaca tgtaccgttt tatgactgag ttcgagttta aaggcgatct
     7381 ctttaacgct gtctcctttg accagtaaat caaacacttc acgttctcgt ggggtgagaa
     7441 tttccagcgc ctgggcgggt tgcccgccgc cgcgtaaagc ccgaatcgca tcggcacaga
     7501 gataatgccc gcccagcccg accgagcgca ccgcctgcac taactcttcc ggcccacagc
     7561 gtttggttaa atacccgctg gcaccggcat ccagcgcgct ttgcacaaag gttggcgcgt
     7621 cataaatgct aagaataatg gcgcgaaatt gcggtttttg tgcacgcagg cgcttaagca
     7681 aactgaggcc gttttcgtcc ggcatggcaa tatcaatgac ggcaacattg acgtcatcac
     7741 ggattaatgc cgaccatgcc tgcgcggcac tactgtactg accaatgacc tccagatcgt
     7801 cttccagaga gagtaattgt gcgaagccag agcgcaccac cacatggtca tccaccagca
     7861 ctacccgaat catacttttc ttctcttatt atctaattct cctatcatgc atcacggcgc
     7921 ggcgctggca atcaaccagc gacagatatg gaatggatgt aacaaaatgg caataatttg
     7981 ccggagcgcg cacatcttat ctggcttgtg aaccggcggg atacgttcgc caaaaccgca
     8041 aaatgcgcaa actcgctaac cattgccgat ttttttctaa aaacgctttg ttcttaacaa
     8101 gacgttttaa ttcctttatc aaactgaccc gcaacgaaat actgctccca taacaagaca
     8161 ggggagcagg caatcatggc aatttcatcg cgtaacacac ttcttgccgc actggcattc
     8221 atcgcttttc aggcacaggc ggtgaacgtc accgtggcgt atcaaacctc agccgaaccg
     8281 gcgaaagtgg ctcaggccga caacaccttt gctaaagaaa gcggagcaac cgtggactgg
     8341 cgtaagtttg acagcggagc cagcatcgtg cgggcgctgg cttctggcga tgtgcaaatc
     8401 ggcaacctcg gttccagccc gttagcggtt gcagccagcc aacaggtgcc gattgaagtt
     8461 ttcttgctgg cgtcaaaact gggtaactcc gaagcgctgg tggtaaagaa aactatcagc
     8521 aaaccggaag atctgattgg caaacgcata gccgtaccgt ttatctctac cacccactac
     8581 agcctgctgg cggcgctgaa acactggggc attaaacccg ggcaagtgga gattgtgaac
     8641 ctgcagccgc ccgcgattat cgctgcctgg cagcggggag atattgatgg tgcttatgtc
     8701 tgggcaccgg cggttaacgc cctggaaaaa gacggcaagg tgttgaccga ttctgaacag
     8761 gtcgggcagt ggggtgcgcc aacgctggac gtctgggtgg tgcgcaaaga ttttgccgag
     8821 aaacatcctg aggtcgtgaa agcgttcgct aaaagcgcca tcgatgctca gcaaccgtac
     8881 attgctaacc cagacgcgtg gctgaaacag ccggaaaaca tcagcaaact ggcgcgttta
     8941 agcggcgtgc ctgaaggtga cgttccgggg ctggtgaagg gaaataccta tctgacgccg
     9001 cagcaacaaa cggcagaact gaccggaccg gtgaacaaag caatcatcga caccgcgcag
     9061 tttttgaaag agcagggcaa ggtcccggct gtagcgaatg attacagcca gtacgttacc
     9121 tcgcgcttcg tgcaataaaa ggaggcgcag atgctgcaaa tctctcatct ctatgccgat
     9181 tatggcggca aaccggcact ggaagatatc aacctgacgc tggaaagcgg cgagctactg
     9241 gtggtgctgg ggccgtccgg ctgcggtaaa accaccctgc tgaatctgat tgccggtttt
     9301 gtgccttatc agcatggcag cattcaactg gcgggtaagc gtattgaggg accgggagca
     9361 gagcgcgggg tggtttttca gaatgaaggg ctgctaccgt ggcgcaatgt acaggacaac
     9421 gtggcgttcg gcctgcaatt ggcaggtata gagaaaatgc agcgactgga aatcgcgcac
     9481 cagatgctga aaaaagtcgg ccttgaaggg gcggaaaaac gctatatctg gcagctttcc
     9541 ggtggtcaac gtcagcgggt ggggattgcc cgcgcgctgg cggcgaatcc ccagctgtta
     9601 ttactcgacg aaccgtttgg tgcgctggac gccttcaccc gcgaccagat gcaaaccctg
     9661 ctgctgaaac tctggcagga gacgggcaag caggtgctgt tgattaccca cgatatagaa
     9721 gaagcggtgt ttatggcgac tgaactggtt ctgctttcac ccggccctgg ccgcgtgctg
     9781 gagcggctgc cgctcaactt tgctcgccgc tttgttgcgg gagagtcgag ccgcagcatc
     9841 aagtccgatc cacaattcat cgccatgcgc gaatatgttt taagccgcgt atttgagcaa
     9901 cgggaggcgt tctcatgagt gtgctcatta atgaaaaact gcattcgcgg cggctgaaat
     9961 ggcgctggcc gctctcgcgt caggtgacct taagcattgg cacgttagcg gttttactca
    10021 ctgtatggtg gacggtggcg gcgctgcaac tgattagccc gctgtttttg ccgccgccgc
    10081 aacaagtact ggcaaaactg ctcaccattg ccggaccgca gggatttatg gacgccacgc
    10141 tgtggcagca tctggcagcc agcctgacgc gcattgtgct ggcgctactc gcggcggtgt
    10201 tgatcggcat tccggtcggg atcgccatgg ggctaagccc gacggtgcgc ggcattctgg
    10261 atccggtaat cgaactttat cgtccggtgc cgccgctggc ttatttacca ctgatggtga
    10321 tctggtttgg tattggcgag acgtcgaaga tcttactgat ctatttagcg atttttgcgc
    10381 cggtggcgat gtcggcgctg gcaggggtga aaagcgcgca gcaggttcgc attcgtgccg
    10441 cccagtcgct gggtgccagc cgtgcgcagg tgctgtggtt tgtcattttg cccggcgcgc
    10501 tgccggaaat cctcaccgga ttacgcattg gtctgggggt gggctggtct accctggtag
    10561 cggcggagct gattgccgcg acgcgcggtt taggatttat ggttcagtca gcgggtgaat
    10621 ttctcgcgac tgacgtggtg ctggcgggga tcgcggtgat tgcgattatc gcctttcttt
    10681 tagaactggg tttgcgcgcg ttacagcgcc gactgacgcc ctggcatgga gaagtacaat
    10741 gagtgaacgt ctgagcatta ccccgctggg gccgtatatt ggcgcacaaa tttcgggtgc
    10801 cgacctgacg cgcccgttaa gcgataatca gtttgaacag ctttaccatg cggtgctgcg
    10861 ccatcaggtg gtgtttctgc gcgatcaagc tattacaccg ctgcagcaac gcgcgctggc
    10921 ccagcgtttt ggcgaactgc atattcaccc tgtttaccct catgccgaag gggttgacga
    10981 gatcatcgtg ctggataccc ataacgataa tccgccagat aacgacaact ggcataccga
    11041 tgtgacattt attgaaacgc ctcccgcagg ggcgattctg gcagctaaag agttaccttc
    11101 gaccggcggt gatacgctct ggaccagcgg tattgcggcc tatgaggcgc tctctgttcc
    11161 cttccgccag ctgctgagcg ggctgcgtgc ggagcatgat ttccgtaaat cgttcccgga
    11221 atacaaatac cgcaaaaccg aggaggaaca tcaacgctgg cgcgaggcgg tcgcgaaaaa
    11281 tcctccgttg ctacatccgg tggtgcgaac gcatccggtg agcggtaaac aggcgctgtt
    11341 tgtgaatgaa ggctttacta cgcgaattgt cgatgtcagc gagaaagaga gcgaagcctt
    11401 gttaggcttt ttattcgccc atatcactaa accggagttt caggtgcgtt ggcgctggca
    11461 accgaatgat attgcgattt gggataaccg cgtgactcag cactatgcca atgccgatta
    11521 cctgccacag cggcggatta tgcatcgggc gacgatcctt ggggataaac cgttttatcg
    11581 ggcggggtga tacgagtgcg gacggttccc tcgccccctt ggggagaggg ttagggtgag
    11641 ggggcgttca ccgtactttc aacaggttaa ctcccccttt ctgagaggaa aaaaattaac
    11701 gcagaatctt cttctcagcc aaatccagcg caaagtagct gaaaatcaga tccgcacccg
    11761 cacgcttaat cgaacctaag ctttcgagca ccactttctc ttcatctata gcacccgcca
    11821 gcgcggcgaa cttaatcatc gcgtactcac cgctcacctg atacgcgcca atcggcaatt
    11881 cagtacgttc acgcagctca cgcacgatgt cgaggtatgc tccagcaggt ttaaccatca
    11941 ggcagtctgc gccctgggct tcatccagca gtgattcacg aatcgcctcg cgacggttca
    12001 ttgggttcat ctgatagctt ttgcggtcgc cttttaatgc gcttccggca gcttcacgga
    12061 acgggccata aaaggaggag gcgaacttgg tcgaatacga cataatcgcc gtatctttaa
    12121 agcccgcagc gtccagcgcc tggcgaattg cctgtacctg gccgtccatc gcggcggaag
    12181 gggcgatgaa gtctgcgcct gcagcagctg caaccacggc ttgcttgcct aaattttcca
    12241 gagtcgcgtc gttgtcgacg ccatgctcgc acagcacacc gcagtgaccg tgagaagtgt
    12301 attcacagaa acaagtgtct gacatgacga tcatttctgg cacagtctgc ttgcagatgc
    12361 gcgacatacg cgccaccaat ccatcttccc gccaggcgtc gctgccggtt tcatcggtat
    12421 ggtgagagat gccgaaagtc atcacggaac gaataccggc gttggcgatg cgttcaattt
    12481 cgcgtgccag atgtttctct ggaatgcgca tcacgcctgg catggcttca acggctttgt
    12541 agtcgtcaat ttcttcttca acaaagatcg gcaacaccag gtcgttaagg ctaagtgttg
    12601 tctcttcaaa catagcgcgc agcgcaggag atttgcgcag gcgacgaggg cgttggatta
    12661 agtctgtcat ggtctgcctg atgtttgtgg aatcgagggg catagtatac ctgaagcagg
    12721 gtggggatgt tttacgaaag ttgtcgcgat gttgacaaga agagaatgga agag
//
LOCUS       DAZUCE010000142        12718 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZUCE010000142 DAZUCE010000000
VERSION     DAZUCE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.142"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            250..2643
                     /gene="malP"
                     /locus_tag="ACHY03_003403"
     CDS             250..2643
                     /gene="malP"
                     /locus_tag="ACHY03_003403"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFS3906595.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFTKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHQAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            2653..4734
                     /gene="malQ"
                     /locus_tag="ACHY03_003404"
     CDS             2653..4734
                     /gene="malQ"
                     /locus_tag="ACHY03_003404"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFS3906596.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHITGGKAFNLPT
                     KLPEGYHTLTLTQEDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRSPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRKAAAKK"
     gene            complement(4779..6095)
                     /gene="gntT"
                     /locus_tag="ACHY03_003405"
     CDS             complement(4779..6095)
                     /gene="gntT"
                     /locus_tag="ACHY03_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFS3906597.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(6456..7031)
                     /gene="nfuA"
                     /locus_tag="ACHY03_003406"
     CDS             complement(6456..7031)
                     /gene="nfuA"
                     /locus_tag="ACHY03_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFS3906598.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(7090..7773)
                     /gene="gntX"
                     /locus_tag="ACHY03_003407"
     CDS             complement(7090..7773)
                     /gene="gntX"
                     /locus_tag="ACHY03_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFS3906599.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     YARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            7811..8581
                     /gene="bioH"
                     /locus_tag="ACHY03_003408"
     CDS             7811..8581
                     /gene="bioH"
                     /locus_tag="ACHY03_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFS3906600.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(8608..9486)
                     /gene="rpnA"
                     /locus_tag="ACHY03_003409"
     CDS             complement(8608..9486)
                     /gene="rpnA"
                     /locus_tag="ACHY03_003409"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFS3906601.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDIT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLEDV
                     LRFTGLSEEELAAASQ"
     gene            complement(9689..9925)
                     /gene="feoC"
                     /locus_tag="ACHY03_003410"
     CDS             complement(9689..9925)
                     /gene="feoC"
                     /locus_tag="ACHY03_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFS3906602.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISKTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(9925..12246)
                     /gene="feoB"
                     /locus_tag="ACHY03_003411"
     CDS             complement(9925..12246)
                     /gene="feoB"
                     /locus_tag="ACHY03_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFS3906603.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(12263..12490)
                     /gene="feoA"
                     /locus_tag="ACHY03_003412"
     CDS             complement(12263..12490)
                     /gene="feoA"
                     /locus_tag="ACHY03_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFS3906604.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
BASE COUNT         3142 a         3222 c         3489 g         2865 t
ORIGIN      
        1 taccttccgc tttttgaaaa tacgcaacgg ccattttttg cacttagata cagattttct
       61 gcgctgtatg gcattgattt gctgctaatc ctgtggtttg cactagcttt aagtggttga
      121 gatcacattt ccttgctcat ccccgcaact cctccctgcc taatcccccg caggatgagg
      181 aaggtcaaca tcgagcctgg caaactagcg ataacgttgt gttgaaaatc taagaaaagt
      241 ggaactccta tgtcacaacc tatttttaac gataagcaat ttcaggaagc gctttcacgt
      301 cagtggcagc gttatggctt aaattctgcg gctgaaatga ctcctcgcca gtggtggctg
      361 gcagtgagtg aagcactggc cgaaatgctg cgtgctcagc cattcaccaa accggtggcg
      421 aatcagcgac atgttaacta catctcaatg gagtttttga ttggtcgtct gacgggcaac
      481 aacctgttga atctcggctg gtatcaggat gtacaggatt cgttgaaggc ttatgacatc
      541 aatctgactg acctgctgga agaagagatc gaccctgcgc tgggtaacgg tggtctggga
      601 cgtctggcgg cgtgcttcct cgattcaatg gcaactgtcg gtcagtctgc gacgggttac
      661 ggtctgaact atcaatatgg tttgttccgc cagtcttttg tggatggcaa acaggttgaa
      721 gcgccggatg actggcatcg cagtaactac ccgtggttcc gccacaacga agcactggat
      781 gtgcaggtag ggattggcgg taaagtgacg aaagacggac gctgggagcc ggagtttacc
      841 attaccggtc aagcgtggga tctccccgtt gtcggctatc gtaatggcgt ggcgcagccg
      901 ctgcgtctgt ggcaggcgac gcacgcgcat ccgtttgatc tgactaaatt taacgacggt
      961 gatttcctgc gtgccgaaca gcagggcatc aatgcggaaa aactgaccaa agttctctat
     1021 ccaaacgaca accatactgc cggtaaaaag ctgcgcttga tgcagcagta cttccagtgt
     1081 gcctgttcgg tagcggatat tttgcgtcgc catcatcagg cggggcgtaa actgcacgaa
     1141 ctggcggatt acgaagttat tcagctgaac gatacccacc caactatcgc gattccagaa
     1201 ctgctgcgcg tgctgatcga tgagcaccag atgagctggg atgacgcctg ggccattacc
     1261 agcaaaactt tcgcttacac caaccatacc ctgatgccag aagcgctgga acgctgggat
     1321 gtgaaactgg tgaaaggctt actgccgcgc cacatgcaga ttattaacga aattaatact
     1381 cgctttaaaa cgctggtgga gaaaacctgg ccgggcgatg aaaaagtgtg ggccaaactg
     1441 gcggtggtgc acgacaaaca agtgcatatg gcgaacctgt gtgtggttgg cggtttcgcg
     1501 gtgaacggtg ttgcggcgct gcactcggat ctggtggtga aagatctgtt cccggaatat
     1561 caccagctat ggccgaacaa attccataac gtcaccaacg gtattacccc acgtcgctgg
     1621 atcaaacagt gcaacccggc actggcggct ctgttggata aatcactgaa aaaagagtgg
     1681 gctaacgatc tcgatcagct gatcaatctt gaaaaattcg ctgatgatgc gaaattccgt
     1741 cagcaatatc gcgagatcaa gcaggcgaat aaagtccgtc tggcggagtt tgtgaaagtt
     1801 cgtaccggta ttgagatcaa tccacaggcg attttcgata ttcagatcaa acgtctgcat
     1861 gagtacaaac gccagcacct gaatctgctg catattctgg cgttgtacaa agaaattcgt
     1921 gaaaacccgc aggctgatcg cgtaccgcgc gtcttcctct tcggcgcgaa agcggcaccg
     1981 ggctactacc tggcgaagaa tattatcttt gcgatcaaca aagtggctga cgtgatcaac
     2041 aacgatccgc tggttggcga taagttgaag gtggtgttcc tgccggatta ttgcgtttcg
     2101 gcggcggaaa aactgatccc ggcggcggat atctccgaac aaatttcgac tgcaggtaaa
     2161 gaagcttccg gtaccggcaa tatgaaactg gcgctcaatg gtgcgcttac tgtcggtacg
     2221 ctggatgggg cgaacgttga aatcgccgag aaagtcggtg aagaaaatat ctttattttt
     2281 ggtcataccg tggaacaagt gaaggcaatt ctggccaaag gctacgaccc ggtgaaatgg
     2341 cggaagaaag ataaggtgct ggacgcagta ttgaaagagc tggaaagcgg taaatacagc
     2401 gacggcgata agcatgcctt cgaccagatg ctgcacagta tcggcaaaca gggcggcgat
     2461 ccgtatctgg tgatggcgga tttcgcagcc tatgtagagg cacaaaagca ggtggatgtg
     2521 ctgtaccgcg accaggaggc ctggactcgc gcggcgatcc tcaataccgc ccgctgcggt
     2581 atgtttagct cggatcgctc tattcgcgat tatcaggctc gtatctggca ggcaaaacgc
     2641 taaggaagct cgatggaaag caaacgtctg gataatgccg cgctggcggc ggggattagc
     2701 cccaattaca tcaatgccca cggtaaaccg cagtcgatta gcgccgaaac caaacggcgt
     2761 ttgcttgacg cgatgcatca acgtaccgcc acgaaagtgg cggtaacgcc agtcccgaat
     2821 gtcatggttt ataccagcgg caaaaaaatg ccgatggtgg tggagggcag cggcgaatat
     2881 agctggctgc tgaccaccga agaaggaacg cagtacaaag gccatataac ggggggcaaa
     2941 gcgttcaatc taccgacgaa gctgccggaa ggttatcaca cgctgacgct tacccaggaa
     3001 gaccagcgcg cgcattgcag ggtgattgtc gccccgaaac gctgttacga accgcaggcg
     3061 ttgctgaata aacaaaagct gtggggtgcc tgcgtccagc tttatacgct gcgatcggaa
     3121 aaaaactggg gtattgggga ttttggcgat ctcaaagcga tgctggtgga tgtggcaaaa
     3181 cgtggcgggt cgttcatcgg cctgaacccg attcatgcgc tctatccggc aaatccggag
     3241 agcgccagcc catacagccc gtcttctcgc cgctggctga atgtgattta tatcgacgtt
     3301 aacgccgtcg aagatttcca tcttagcgaa gaggctcagg cctggtggca gttgccgacc
     3361 acgcaacaga cgctgcaaca ggcgcgggat gccgactggg tcgattactc cacggttacc
     3421 gccctaaaaa tgacagcatt acgaatggcg tggaaaggtt tcgcgcaacg tgatgatgag
     3481 cagatggccg cgtttcgcca gtttgttgca gatcaaggcg acagcctgtt ctggcaggca
     3541 gcctttgatg cgctacatgc ccagcaagtg aaagaggacg aaatgcgctg gggctggcct
     3601 gcatggccag agatgtatca gaacgtggat tcaccagaag tgcgtcagtt ctgcgaagaa
     3661 catcgcgatg acgttgattt ttatctctgg ctgcagtggc tggcttacag ccagtttgcc
     3721 gcctgctggg agataagcca gggctatgaa atgccgattg gcttgtatcg tgatctggcg
     3781 gttggcgtag cggaaggtgg ggcggaaacc tggtgtgacc gtgaactgta ttgcctgaaa
     3841 gcgtcggttg gcgcgccgcc ggatatcctc ggcccgttgg ggcagaactg gggattaccg
     3901 ccaatggacc cgcatatcat caccgcgcgt gcctatgaac cgtttatcga gctgttgcgt
     3961 gccaatatgc aaaactgcgg cgcattacga attgaccatg tgatgtcgat gctgcgtttg
     4021 tggtggatac cttatggcga gacggcggat cagggggcat acgtacacta tccggtggat
     4081 gatctgctct caattctggc actcgaaagt aagcgtcatc gctgcatggt gattggtgaa
     4141 gatctcggta ccgtaccggt agagattgtc ggcaagctgc gcagcagcgg tgtttactcc
     4201 tacaaagtgc tctatttcga aaacgaccac gagaagacgt tccgttcacc gaaagcgtac
     4261 ccggagcagt cgatggcggt tgcggcgaca cacgacctgc caacgctgcg tggttactgg
     4321 gagagcgggg atctaactct gggcaaaacc ctggggctgt atccggatga agtggtactg
     4381 cgcggtctgt atcaggatcg cgaactggcg aagcaagggc tgctggatgc actgcataaa
     4441 tatggttgtc tgccaaaacg tgccgggcat aaggcatcgt tgatgtcgat gacgccgacg
     4501 ctgaaccgtg gtttgcagcg ctacattgcc gacagtaaca gtgctctgtt aggactacag
     4561 ccggaagact ggctggatat ggccgaaccg gtgaatattc ctggcaccag ttaccagtat
     4621 aaaaactggc gacgcaagct ttccgcaacg cttgagacga tgtttgccga tgatggcgtg
     4681 aacaagttgc tgaaggattt ggacagacgg cgcaaagcgg cagcgaagaa gtagagtgcg
     4741 gttgatgccg gatgcgatgc atccggcagt cagtgttatc aaatcaccat attcagcagc
     4801 agacagccta ccagaccgca caccgagata atggtttcca gcatcgacca ggatttgatg
     4861 gtctcgccga tagtcaggtt aaagtactct ttgaacagcc agaagcccgg atcgttcaca
     4921 tgagagaaaa tcacactacc ggaacccacc gcaataacca tcagctccgg gctaacaccc
     4981 gtcgttgcaa tcagcggtgc cgcgatacca cccgcagtga ttgccgcaac ggttgcggaa
     5041 cccagcgcga tacgcagtac ggcagcaatc gaccaggcca tcagcagcgg agaaatgttg
     5101 gtttcgtgca tcatggaagc aatgtatttg tccacgccgc tgtctaccag cacctgcttg
     5161 aacgcaccgc caccaccgat gatcaacagc atcatcgcaa tgattttgat ggaagaaacc
     5221 agcgtgtcgt taatctgatc cattgaacga ccacggttta gaccaaaggt gaacatcgca
     5281 atcagcacgg caatcagcgt agccattacc gggtcaccga ggaactccgc taccggcagg
     5341 aaagcgtgac ctttcggcag gatcatttcg gcaatcgcac gcatcgccat cagtactacc
     5401 ggcaccagag aggtccagac gctgacgcca aagctcggca tctcttcttc gctgaaggtt
     5461 ttcgcgctgt agagaccttc cggaattggc ttatcgatac ctttcaacac gcgagcgtaa
     5521 accggaccgg cgagaatcac ggtcgggatt gccagaatag taccgtacag cagggtttta
     5581 cccatgtcgg cattgaaaat ggtggcaatc gcagtcgggc ccggatgcgg tggcaggaag
     5641 ccgtgggtca cagacagtgc agctgccatt ggtacaccaa catacagcag tggaatattc
     5701 gcagaagccg cgatggtaaa caccagcggc agcatcagca caaagcccac ttcatagaac
     5761 agggcaaaac caacggtaaa accggtcagt accaccgccc actggatgtg ttttttacca
     5821 aatttggcaa tcagcgtggt ggcgatacgt tgtgcgccac cgcagtctgc cagcattttg
     5881 cccagcattg cgccaaaacc catgatcagg gcaaggctac cgagcgtccc gccgacaccg
     5941 gctttgatgg agccaataac tttatccagc ggcattcctt gcattaatcc aacagcaagc
     6001 gccacgagga cgagagcgat gaagccgttc attttgaagc ggatcatcag gagcaacaac
     6061 aagattacac cgatagcaac aatgactaat ggcatgattt acctggcctt tcatttgtta
     6121 tgggtaacgt caattttctg acgacaaact ctaattatcc caatcgggaa cagagatatt
     6181 gcggcaccac gactgatacc cactaaaact aattattgta gtcagatgtc aggagtatgt
     6241 ttggtaccca tgtgaatgat acgggtaaca tctggcgttt gagaatcacc agagcggggt
     6301 aaatttaaat tatgagaggt tggtcatatt atcgcgggga aacgaaggga gggatttgcc
     6361 aaagcaatgc tgcgccaacg tctggcacat gttcaacgta ggcccgaaat gacgctttag
     6421 cgtcgcatcg ggcaatctac aaaagagggg ataacttagt agtaggagtg ttcgccgcgc
     6481 tggtgttcgg tgagatcgcg cacacctttc agctccggga attcgtttag cagctgcttc
     6541 tcgatccctt ctttcagcgt cacatcgacc atggaacaac cgttacagcc gccgccaaat
     6601 tgcagaatgg cgtaaccgtc ttcggtgatt tccatcagtg aaacgcgacc accgtgacca
     6661 gcaagctgtg ggttgatctg cgactgcagc atatactcca cgcgctccat cagcggtgca
     6721 tcgtctgcca ctttacgcat tttggcgtta ggcgctttca gtgttagctg agaacccaac
     6781 tggtcggtaa caaaatcgat ctctgcatct tccaggtatg gtgcgcttaa ctcatcaaca
     6841 tacgcggtca gcaggtcaaa tttcagggca gtgtcggtgg cttccacggc atccggcgga
     6901 caataagaaa caccacattc agcgttaggc gtgccagggt taatcacaaa tacgcggatt
     6961 tgtgtccctt cttcctggtt tgccagcagt ttggcaaagt gcgcttgtgc agcatcggaa
     7021 atacggatca tagtaatggc ctaatagttg actattttag ttggttataa tacgcccatc
     7081 atcgaggctc tacaaggttc gacaaaggca ccagacctgg acagtcgccg caccattgcg
     7141 taaaagcaac tgcgcaatct ctgcgacggt acttccggtg gtaacgacat catccacaat
     7201 caccatatgg cgaccttgca cgggcaattc aagacgaaag gcatttttca ggttgcgctt
     7261 gcgtagccgg gcactgagaa aatgctgggt cgcagtggcc cgtgttcgtg tgacggcttc
     7321 gctatcccat cggcagtgca accagcgtga taacggctga cacagcaaat cgctctgatt
     7381 aaatccccga cgccagtgac gccgctgcca taacggaacg ctgatgatgc gatccggcaa
     7441 ttgcaacccg gtggtgcgac gagcgtataa gacctccaat agtaacagac gtgacagggc
     7501 gctggcgatt tcgctgcgcc gggaaaactt aagctggtgg attagcgggc ttaacggcgg
     7561 cgcatagtcg gcaaccgtga ccagtctttg ccagggcggt ggtttttgca ggcagcgacc
     7621 gcagggaaga tgtgagtgtg tggcgggtaa tccacattgt gggcataacg ttttatctgt
     7681 acgggcggcg cgtgagcaga ccgaacaaat cccccaatga cctaacgcca gcggcattcg
     7741 gcatagccag cataatcctg gtactgttag catatgttca tccttgtaag tcaaaagaga
     7801 acaatagcgg atgaataaca tctggtggca gaccaaaggt caggggaatg ttcatcttgt
     7861 gctgctgcac ggatggggac tgaatgccga agtgtggcgt tgcattgacg aggaactgag
     7921 ctcgcatttt acgctgcacc ttgttgacct gcccggcttc gggcgtagcc ggggatttgg
     7981 tgcgatgtca cttgctgata tggccgaagc cgtgctgcga caggcacctg ataaagccat
     8041 ttggttaggc tggagtctgg gcgggctggt ggcaagccag attgcgttaa cccatcccga
     8101 gcgtgttcag gcgctggtca ccgtggcgtc gtcaccttgt tttagtgctc gtgacgagtg
     8161 gccggggata aaaccggacg tgctggcggg atttcagcag caactcagtg atgattttca
     8221 gcgtacggtg gaacggttcc ttgcgttaca aaccatgggt actgaaacgg cgcgtcagga
     8281 tgcgcgggcg ttgaagaaaa ccgttctggc attaccgatg ccggaggttg acgtgctcaa
     8341 tggtgggctg gaaatcctga aaacggtcga tctccgccag ccgctgcaaa acgtacccat
     8401 gccgtttttg cgactgtatg gttatctcga cggtctggtg ccgcgcaaag tggtgccgat
     8461 gctggataaa ctctggccgc acagcgagtc gtatatcttc gccaaagcgg cccatgcgcc
     8521 gtttatttcg catccggtcg agttttgtca cctgctggtg gcgttgaagc agagggtgta
     8581 ggcacttttg aatggcgaga cagaacttta ctgactcgcc gcagccaact cttcctctga
     8641 cagcccggta aatcgcagaa cgtcctcaag agggactccg gattcaagca ttattttggc
     8701 tatatgcagg gctttggatt gttccccctc ctgccgcaat ctttccgcaa tagtcattaa
     8761 actctccttg tgtttcggtg atcgttcggc aacgccgtcg ataaaatcgt taaaacgtac
     8821 cgcatcgccg gtttgcagta tgtaattaaa cagccctttg atttgtctgt cattagcgta
     8881 tccactactt aataagcagg ccatttgctc taccagcccc atcaggtcgc gttgacgaat
     8941 atgtttttga attaactcca gcagcgccat gcgtcggtgc tgcatgattt catcatcagg
     9001 catgacggtg atatcaatca gcggaaatgc ggaggcataa agctgccttg ccagtttggg
     9061 atcggcgaaa caatccagcc aacacagcga atagggatag gggctttcaa taccgtggta
     9121 aaacaacaat ggcaccacca tcggcaacgt tttgtatccg gcatcaaggt gattttgcat
     9181 tgcggcaata gcgtaacgca tcatgcgaaa tgcgatcagt ttgtttgagg tgctttgatg
     9241 ttcaatcaga caatagatgt atcctggtcc ttgttctgtt ttcaccgacc acagcacatc
     9301 ggaatagctt tcacgcagat cgtcatcaat aaagctgctc gactccagct tgagggtttt
     9361 catatcacag agcgcgtgaa tgggtgccgg taaatgaaac gcaagaaaat cgcgtgccgt
     9421 ctccggttgg cgtaaaaaga gtttgaacag cgcatcgtgt ggggtggaac tctgcttttt
     9481 gctcatggcg ttccttcacc tgttgtttga atgccgcgac tctacgcgcc actccacacc
     9541 tgtgcattag ccagttttca tccctgcgag acgctttcag aggaacttac gtcgcgctgc
     9601 aagaattgca tttttatgga aagccgcacg caaaaaagcc cggatgcaaa cgcataccgg
     9661 gcttttcaaa acaggcgatg gagtatggtt aacgcagcgc ccaccactcg cgcagacagg
     9721 cttttccttc cgggcagctt ttacaactgc cggagaggca gccgtcaggt tcttcctgaa
     9781 tccgcacggc tttgcccata ctttccagtt gttgcagcat ggcgttaatc attggctgtg
     9841 gagtgttcaa tgtcttgctt atctgggccg cttccatacg gccccgtaac gccaacaaat
     9901 cacgcacctg aataagtgaa gccattaatg gcaatcaccg gtggtgctgg ctgcgcagca
     9961 actacttacc gacttgcggg ttgccagcag ttcgatatcc acccggctac gcgcgcggcg
    10021 cagcagaccg ataaccacga tgttaaacag gataaccgcc agaatgcaca ccaggctgta
    10081 agttggatgc tggctgtagc tggcgacttg atagaacaat gttgccagcg agtaagcgat
    10141 attcagcccc cacaggatgg agaagcccat ccagccacgg cttgattcac gggcgatggc
    10201 tcccatcacc gagatgcacg gtacatacag caggacgaaa atcaggtagc tgtatgcggc
    10261 agctgcgcta ccgaatttct gatccatcac acccatcgcc ccggtaccca tttcgccgtc
    10321 gcctttgcta gcttcaatgg ggttcatcag tacgctaagg ctgaaggtgt ctttcaggct
    10381 ctgccaggtt tcatctaccg cgctgaacaa ctcttcaccg aggttaaatt ccgccggatt
    10441 gaactcttcg tcctgaatat tttctgcggt gtagagggtg ttgagcgtac ccaccaccac
    10501 ttctttcgcc attgcacccg taaacaggcc aactgttgcc tgccagttat cttcatgcac
    10561 gccaatcggc ttgaagaccg gggtgatcac ccggctgacg gaagccagcg ccgagtcgtt
    10621 gatgttatcg acgattttcc cgctcaacga gaagctgttg aaagcgctca ggaaaatgct
    10681 gacgatgata atcactttac cagcacgcag aacgaagcct ttcagacgct gccaggtctg
    10741 gataatcagg cttttaacgt gtggtacatg gtagaccggc agctccatga caaacggcgt
    10801 cgcttcaccg cgcatgatgg tgtacttgag catcaggcca gtcagcaccg ccatcacgat
    10861 accgagcata tacagcgaga agaccgccag cgcaccgttc tgcccgaaga aggcagccgc
    10921 gaatactgcg aagatagcca gacgcgcgcc gcaggacata aacggtgcca tcatgatggt
    10981 catcagacgt tcacgcggtg catcaagtgt acgtgcaccc attaccgacg gtacgttaca
    11041 accgaaaccg acgatcagcg gcacaaagga tttccccggc aagcccagcg cctgcatcag
    11101 gcggtccatc acaaacgctg cacgcgccat atagccggag tcctcaagga aggagaggaa
    11161 caggtacatc atgccgatct gcggcaccag tggcagcacg gtgttaatac cgccacccag
    11221 gccctgggcg aggaagatag tcagccagtc cgggaagtgg agcgtgtagc caatccattg
    11281 aataccatgc acaaatagcg ccacggagcc gacgtcaaac agcggctgta acgccccgcc
    11341 gatgttgata gccagcagga acatcaggta catcacaaag aggaaaatcg gcagaccgag
    11401 gaaacggttg agcacgattt tatctaccgc agtggtgaaa cggctgggtt ctgccgtcag
    11461 ggtgttgctt accacatcac agatggcagc aatgcactgg taacgcgcat cggcaatgtg
    11521 cagcgccgga tcgtccatct cattacgcag acgggcgagg gcggcatcaa gatgctgcga
    11581 cgcttcaccg gcgtaggcgc ggctgtagat atcgccttcc agcatttgca ggcccagcca
    11641 gcgacgttgt ttcagcggga tgtcggaagg catcactttt gccagtgaat ctgcttcgtt
    11701 gagcagcggc tgtgcgtaat gcaccagttc cacattctcg ttagctttat agcgatcaat
    11761 cgccagcttg agcgcttcaa taccgcgacc acgggttgaa accagcggga tcactgggca
    11821 gccaagacgc gccgacagag catcaatttc aatacgaata ttttgcttct cggcaatgtc
    11881 gagcatgttc agtgccacga tgcagggaat gccgagttcc agcagttgta gcgtcaggta
    11941 caggttacgc tcaaggttag acgcatccac cacgttaatc agcaggtcgg cgtcgccact
    12001 caaaatgtag tgacaggcga tttgctcatc gagcgaggtc tgcgatgaga tggtggtcag
    12061 agaataggtg ccgggcaggt ccaccagcgt gacctgatga tcggtggtgg agaattgccc
    12121 ttctttacgt tcgacggtaa cgccagccca gttacctaca cgctgacgtg cgccagtgag
    12181 ctggttaaat aaggttgtct tgccagaatt tggattacca attaagccta tggttaattt
    12241 tttcattgtt gttatcaccg tattaacagg aaaccgcttc cacttctaat aaggccagat
    12301 cttttttgcg taataccagg ctcacacgac gggtttcgat atgaatgggg tcgccgagtg
    12361 gagcgacgcg caccacatta aaagaggagc caggtaacat gccaagagaa agcagttttt
    12421 ggcgatatgc cgggctgatt tcacgggaaa agccagtgat tttccacgca gtatctggag
    12481 tgtattgcat aggtgcctac ttgtttctca ttaactggat aactacctca tggggcgttt
    12541 gacagcgaca tgagaccaga tgatttgcca acgaaggaga gattaaataa tcaacagtac
    12601 aatgataatg agaatggttt ctatcagcaa tacttaaaat gtgtgcgaat gttggtttga
    12661 ataataatct gactcaatgt tgatgtggct caaggttata tcgcagcgaa aatataat
//
LOCUS       DAZUCE010000143        12718 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZUCE010000143 DAZUCE010000000
VERSION     DAZUCE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.143"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            200..1069
                     /gene="robA"
                     /locus_tag="ACHY03_003413"
     CDS             200..1069
                     /gene="robA"
                     /locus_tag="ACHY03_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFS3906605.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(1066..1713)
                     /gene="gpmB"
                     /locus_tag="ACHY03_003414"
     CDS             complement(1066..1713)
                     /gene="gpmB"
                     /locus_tag="ACHY03_003414"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFS3906606.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            1765..2277
                     /gene="yjjX"
                     /locus_tag="ACHY03_003415"
     CDS             1765..2277
                     /gene="yjjX"
                     /locus_tag="ACHY03_003415"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFS3906607.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(2424..2750)
                     /gene="trpR"
                     /locus_tag="ACHY03_003416"
     CDS             complement(2424..2750)
                     /gene="trpR"
                     /locus_tag="ACHY03_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFS3906608.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(2840..4777)
                     /gene="sltY"
                     /locus_tag="ACHY03_003417"
     CDS             complement(2840..4777)
                     /gene="sltY"
                     /locus_tag="ACHY03_003417"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFS3906609.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            4988..6655
                     /gene="ettA"
                     /locus_tag="ACHY03_003418"
     CDS             4988..6655
                     /gene="ettA"
                     /locus_tag="ACHY03_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFS3906610.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(6762..6929)
                     /gene="nadS"
                     /locus_tag="ACHY03_003419"
     CDS             complement(6762..6929)
                     /gene="nadS"
                     /locus_tag="ACHY03_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFS3906611.1"
                     /translation="MSFFDELKTSLEETVEIKQSLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(6963..8195)
                     /gene="nadR"
                     /locus_tag="ACHY03_003420"
     CDS             complement(6963..8195)
                     /gene="nadR"
                     /locus_tag="ACHY03_003420"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFS3906612.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEEDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(8216..9598)
                     /gene="radA"
                     /locus_tag="ACHY03_003421"
     CDS             complement(8216..9598)
                     /gene="radA"
                     /locus_tag="ACHY03_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFS3906613.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(9647..10615)
                     /gene="serB"
                     /locus_tag="ACHY03_003422"
     CDS             complement(9647..10615)
                     /gene="serB"
                     /locus_tag="ACHY03_003422"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFS3906614.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            10721..11365
                     /locus_tag="ACHY03_003423"
     CDS             10721..11365
                     /locus_tag="ACHY03_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFS3906615.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            11393..12409
                     /gene="lplA"
                     /locus_tag="ACHY03_003424"
     CDS             11393..12409
                     /gene="lplA"
                     /locus_tag="ACHY03_003424"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFS3906616.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            12441..12704
                     /locus_tag="ACHY03_003425"
     CDS             12441..12704
                     /locus_tag="ACHY03_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3906617.1"
                     /translation="MIVLENQEREIINLMFSQHISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
BASE COUNT         2929 a         3569 c         3299 g         2921 t
ORIGIN      
        1 ttttatctat tacacgagca attagttcgt cacggtaaat aacatattga ttaagaacaa
       61 aatctcaata cttttatttc cgtcaagacc taaaacatac tctactaaga aaaaaacact
      121 gaatgctaaa acagcaaaaa atgctattat ccaattacct gatgtcaggt gctcgttgtt
      181 gaaaggatga ggatatttta tggatcaggc cggcattatt cgcgaccttt taatctggct
      241 ggaaggtcat ctggatcagc ccctgtcgct cgacaatgta gcggcgaaag caggttattc
      301 caagtggcac ttacagagaa tgtttaaaga tgtcactggc catgctattg gcgcgtatat
      361 tcgtgctcgt cgtttgtcga aatcggcggt cgcactacgc ctgactgcgc gtccgattct
      421 ggacatcgcg ctgcaatacc gcttcgactc tcaacagaca tttacccgcg cattcaagaa
      481 gcagtttgcc cagactcctg cactttaccg ccgttctcct gaatggagcg cctttggtat
      541 tcgcccgccg ctgcgtctgg gtgaattcac catgccagag cataaatttg tcaccctgga
      601 agatacgccg ctgattggtg ttacccagag ctactcctgt tcgctggagc aaatctctga
      661 tttccgccat gaaatgcgtt atcagttctg gcacgatttt ctcggcaacg cgccgaccat
      721 tccgccagtg ctctacggcc tgaatgaaac gcgtccgagt caggataaag acgacgaaca
      781 agaggtattc tataccaccg cgttagccca ggatcaggca gatggctatg tactgacggg
      841 gcatccggtg atgctgcagg gcggcgaata tgtgatgttt acctatgaag gtctgggaac
      901 cggcgtgcag gagtttatcc tgacggtata cggaacgtgc atgccaatgc tcaacctgac
      961 gcgccgtaaa ggtcaggata ttgagcgata ctacccggca gaagacgcca aagcgggaga
     1021 tcgcccaatt aatctgcgct gtgaactgct gattccgatc cgtcgttaac gctgcagctc
     1081 atctaatgca ggggcgtcca gatgcgagat gtcccctgcg gtttcaacga cccagccaga
     1141 cgccagccac aggctttcct gataatccac gcgcgaaata gaacagttac gcagacgtaa
     1201 gcggcgctct gcccatgctg gtaatccgag aatcgtactc accaggcatc ccagtgcaat
     1261 accatggctc accagcagcg gtcggcttcc ctgcggtaag tcccggcaag attccagagc
     1321 cgcattgacg cgatcgctga gctcctgcat tgactcgcct tcaggaatac gcccgtcaac
     1381 ggtgccattg accagctgcc gacgccagtt ctcttcttct tcggtcagag aatcgatatg
     1441 tcttttttcc agcacaccca tgtttaattc acgcaggcga gaatcaaaga tgatgtcaca
     1501 gccgcaggcc tgggcgatga tttccgccgt acgccgggtg cgtcctaaat cgctactgat
     1561 gatatgcgta atgccaagct ctttggcacg ggttgccacc tgcatcgcct gttgctcacc
     1621 tttggcggtc agcgggctgt cagactggcc ctgaatacgt cgctcggcgt tccactgcgt
     1681 ttcaccgtgg cggacaaggt atacctgtaa catgcttttt ttccgtatac tgctgtaacg
     1741 ttaattttct gagtaatgct gattatgcac caagttgtct gtgcgaccac caatcccgct
     1801 aaaattcagg ccattctgca ggcatttcac gagatcttcg gcgaaggatc ctgccatatt
     1861 gcatccgttg ccgtcgagag cggtgtaccg gaacagccct ttggcagtga ggaaacgcgc
     1921 gctggcgcac gaaatcgggt agcaaatgcc cgccgtttac ttccagaggc tgatttttgg
     1981 gtggcgattg aagctggcat cgatggtgac agcactttca gttgggtggt gattgaaaac
     2041 gccagccagc gcggcgaggc gcgttcggct actttgccgt tgcctgcggt gattctggaa
     2101 aaagtgcgtg aaggtgaggc gcttggtccc gtgatgtcgc gttataccgg tattgatgag
     2161 attggtcgta aggaaggggc gatcggcgtg tttaccgccg ggaaactcac tcgcgccagc
     2221 gtctatcacc aggcggtgat cctggcgctg agtccgtttc ataatgccgt gtattaagcg
     2281 cctgatgcga cgctgccgcg tcttatcatg cctaccaaac atattgaaat tacgggtatt
     2341 tgtaggacgg ataaggcgtt cacgccgcat ccggcattcg gtgcacgatg cctgatgcgc
     2401 cacgtcttat caggcctaca aaatcaatcg cttttcagca acacctcttc cagccactgg
     2461 cgcagctcaa cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt cgcgatgccc
     2521 gcgccgagtt cattttttaa ctcacgctgg ctcatttcgc cgcgcaacag ctcttcgaca
     2581 atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag gtttaacaac
     2641 ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg taaccactcc
     2701 tggtgacgct gttctgccat cgctgctgaa tagggtgatt gttgggccat aatatgtcgc
     2761 cattgttagc gggggaagca aaatgcctcc cccggttgta ctcgctaaag agtacgatag
     2821 catatcataa acgtgtggat cagtaacgac gtccccattc cgtggcgctc atcaacgtcg
     2881 gtttatcccc catgaaatag cggtagtaag cgtcataagc cagcacgttc ttcacataac
     2941 cgcgcgtctc ggagaacgga atactctcga caaatgccac agcgtcgata cgcccggcgc
     3001 tgttgccaag ccaggttcgc acccgccctg gtccggcgtt ataagctgct gaggagaaaa
     3061 tacggttatt accaaattgc tgataaacat attgcaggta actggtgcca atgttgatat
     3121 tcgtttccgg atccagcaat tgcccagggc tgctataacc aggaatagag aacatcttca
     3181 ccgtatgggt cgccgtgccc ggcataatct gcatcaggcc gctggcccct accggtgatt
     3241 tcactttcgg attccaggcg ctctcctgac gggcaatcgc catcgcatag ctttgcggga
     3301 tctccttccc actggtgtaa cgtttgaaaa gatcgttgta agccagcggg aatcgctctt
     3361 ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc caccattggt
     3421 tgttgaaagc ataccgcgcc agttgagctt gctctgtttt tgacttgctc ttcaccagat
     3481 tggcccactc gctacgcgcg gtgttatcga gattccagta catcaactcg cgcacgcgcg
     3541 ccatctccgg cccctgagtc agggcgctgt caacattctg cggcgcttta tcaatcttca
     3601 gctcatactc ttctccgatg cgttgcgctg caaccatcgg gtagaaacca cgctgttgca
     3661 tgagttgatg caaaatctct tttgcttcag cttcacgtcc gcgttccagc aataaatccg
     3721 cctgccagta acgccattca tctttctctt tcgcctccat cggcaaacgc gccagccagg
     3781 tattcaggcc gcgacgatcg ccagtgccaa gcgccattcg cacacggcgt tcaataagcg
     3841 aagtagattg cgagcgcata atggcgtcat cgcgccattt cgcctgctcg tcggtgacat
     3901 cgttgcccat caaacgccag gcgacgatat cgcgcagctc ctgaatctga tcttcattaa
     3961 gctgctgcgc ctgggcaagc gatgggatca tcagccgtgc attttcagca tcctgccgcg
     4021 ccacactggc aaacgccacc gccgccattt gacgggtaaa atcggtcgct ccggttgtac
     4081 gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga aatgattgcc gaggcgatag
     4141 tctggtaatc ggcaggcatc tgccctgcca gcactgttac caggcctgtg ttacccgctt
     4201 tcatcgccag acggatacgc tctaaatacg ccagcggatc ttgtttacct gacgcacgcc
     4261 agacgctaaa taacttgtca caggcgttag gctggctctt gccggttagc cacagctctt
     4321 tcgccccttg ccaggcttct tcactctgcc cggtgttcca tttcgcatag tagtaattac
     4381 attgcgcttc ggtagttccg ggcttttccg ggctaaaggc taacaagcca cgccagtctt
     4441 cacgccgcgc cagttcattg acgaaacgag attgcagcgt gcgagcggga ggaagcgtgg
     4501 ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg attcatcaga tcatcggtga
     4561 tctggcggta ttccaggtag ggataaagcg gataatcctt cagtccaggc atcatttgtt
     4621 cgaccacatc catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa cgactacgct
     4681 gctcatccag tgagtcggct cgcgccacgc tgctgaccgt cagcagacag acaccggcag
     4741 ccaacagccg ccaggtaact tgtttggctt tttccacaag cgcatcctct aagtgtaaat
     4801 acatcaatgc agcgtcatgc cgcgtaatag atttatgcta accagtcatt gccgtttacg
     4861 ccacgttacg gacacttttt tacttttact gcgaaggtga tcgaccaact gggattaggc
     4921 ggggaaaaag gctacacttc gcctttgaaa actcatcatc acgataaaca tagaggcgaa
     4981 gtccaacgtg gctcaattcg tttataccat gcatcgtgtc ggcaaagttg ttccgccgaa
     5041 acgtcatatt ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa ttggtgtcct
     5101 gggtctgaat ggcgcgggta agtccaccct gctgcgcatt atggcgggca ttgataaaga
     5161 catcgaaggt gaagcgcgtc cgcagccaga catcaagatt ggttatctgc cgcaggaacc
     5221 gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt cagaagtggt
     5281 taacgccctg aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg atgccgattt
     5341 tgacaagctg gccgctgaac aaggccgtct ggaagagatc attcaggctc acgacggtca
     5401 taatctgaac gtacagctgg agcgtgcggc ggatgcgcta cgtctgccgg actgggacgc
     5461 gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgtg gcgttgtgcc gcctgctgct
     5521 ggaaaaacca gacatgctgc tgctcgacga accgaccaac cacctggatg ccgaatccgt
     5581 ggcctggctg gaacgcttcc tgcacgactt cgaaggcacc gtggtggcga ttacccacga
     5641 ccgttacttc ctcgataacg ttgcgggctg gatcctcgaa cttgaccgcg gtgaaggtat
     5701 tccgtgggaa ggtaactact cctcctggct ggagcagaaa gatcagcgtc tggcgcagga
     5761 agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt gggtacgtca
     5821 gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg aagagctgaa
     5881 cagcaccgaa taccagaaac gtaacgaaac caacgaactg tttattccac ctggaccgcg
     5941 tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggcg atcgcctgct
     6001 gattgatgac ctgagcttct cgatcccgaa aggggcgatc gtcgggatca tcggtccgaa
     6061 cggcgcgggt aaatcgaccc tgttccgtat gatctccggt caggaacagc cggacagcgg
     6121 caccatcact ttgggtgaaa cggtgaaact ggcatcggtt gatcagttcc gtgactcaat
     6181 ggataacagc aaaaccgttt gggaagaagt ttccggcggg ctggatatca tgaagattgg
     6241 caacaccgag atgccaagtc gcgcctacgt tggccgcttt aactttaaag gggttgatca
     6301 gggtaaacgc gttggtgaac tctccggtgg tgagcgcggt cgtctgcatc tggcgaagct
     6361 gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca accaacgacc tggatatcga
     6421 aaccctgcgc gcgctggaaa acgccctgct ggagttcccg ggctgtgcga tggttatctc
     6481 gcacgaccgt tggttcctcg accgtatcgc cacgcacatc ctggactacc aggatgaagg
     6541 taaagttgag ttcttcgaag gtaactttac cgagtacgaa gagtacaaga aacgcacgct
     6601 gggcgcagac gcgctggagc cgaagcgtat caagtacaag cgtattgcga agtaatgcgt
     6661 aaaatgccgg atgcggcgcg aacgccttat ccggcctaca aaaccagcaa tttcaatacg
     6721 ttgtaaaact gtaggcctga taagacgcgg taagcgtcgc atcaggcatt gagcaccgaa
     6781 tgccggatgc ggcgtgaaca ccttttccgg tctacaacag ccttagcatc ctcaatttcg
     6841 tggcgggtca cccgtgcagg ttttttcaaa ctttgtttaa tctcgacagt ctcttccaga
     6901 gaggttttca actcatcaaa aaaactcatt ttatacctcg cctttgagca gtttcgtcac
     6961 ggttatctct gctcccccat catctcccgc accagctcca cgcagcgcag gaaacggctg
     7021 tcgtaatctt cctcttcaac ccgcacaaat tcgatattgt tctcttccag catctccacc
     7081 agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa accatccgcc
     7141 acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc atcaattaac
     7201 gcctgcacga acggatgctc acgcccttcg tactttttgc agaacgcctg ggtagtgaca
     7261 aaatcagtgt caataaacgc cactttatta gcatatttca ctgcaaaatc aatgtattga
     7321 gcgtggccca gcgcgatttt atcgtagtcg gaatactgca atgcgatctc atcaccgccg
     7381 aggtgtgaaa agacataatc gcgaccatat tcccacgcac tggtggtatt gaagatattg
     7441 gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc cgccaaggat cgccacggta
     7501 cgcacaaaga acggcttcac ttccgtagga atatattccc agtagcggaa cgggttttcg
     7561 cggatctgtg caccgctgat actcataaag gtacgtttcg gatctaccag caccgtttcg
     7621 atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta gatcaggtcc
     7681 ggctgaatcc ccttttcagc cataaacttt ttgatgccgt tgctccacac atcccagccg
     7741 tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa tgcgaatatt tttctgatat
     7801 ttaaaagttt gcaataacca gcgcagacga tccggcacgg tcggctgctg cgacatggca
     7861 ctgtcttcga acaacgcgcg gtcacgggta tcgtcaaaac ccataatgat atgcagctcg
     7921 tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag tgggtagaac
     7981 ttaccgaata cgacaccgat cgttttcttc tgccggggaa actcaagccc caaaaaacgg
     8041 tgcaacgcct ccagcttttg cgcgctgggg cttttgattt tggcattcag taactggctt
     8101 aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca gccctgttgc
     8161 ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatt tctcattata
     8221 agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc tgcatccctt
     8281 ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa ccgtgtttcg
     8341 ccgcttcaga gattcgttcc tgaccgctgg gcaccgggcg gatctcccct gccagcccga
     8401 cttcaccaaa caccaccaga tcctgcggta gcggtctgtc acgcaggctg gaaaccatcg
     8461 ccagcagtaa cgctaagtcg gcgctggttt cggttacctt cacgccgccg accacgttca
     8521 caaacacatc ctgatcggcc atttgcagac caccgtgacg gtgcaagaca gccagcagga
     8581 ttgccagacg gttttgttcc agccccactg ccacgcggcg cggattcgcc atcatcgagt
     8641 gatcgaccag cgcctgaatc tccaccagca acggacgcgt tccttcccac accaccatca
     8701 cggagctgcc ggaggtcact tcatcgccac ggcttaaaaa gattgccgaa ggattgctga
     8761 cttcacgcag cccctgttcg gtcatcgcga atacgcccag ctcattcacc gcgccgaagc
     8821 ggtttttatg gctgcgtaag gtgcgaaaac gggagtcggc atcgccatcc aaaagcaccg
     8881 aacagtcgat acagtgttcc agcactttcg ggccagccag cgaaccatct ttggttacgt
     8941 gccccaccat gacaatcgcc acaccgcgcg ttttggcgaa gcgtgtcaaa taagccgccg
     9001 tttcacgcac ctgcgccacg ctaccaggcg atgactgtac atccgccata tgcatcacct
     9061 ggatcgagtc aattaccatc agcttcggtt gctcttcttc ggcaatcagg cagatctgtt
     9121 cgatgctggt ttccgacaac atattgagat tgtcagtcgg caggccaagg cgatgagcgc
     9181 gcattgccac ctgttgcagc gactcttcgc cggtgacata cagcgttttc atctgctggg
     9241 ccagtttgca cagcgtttgc agtagcagcg tggatttccc cgcaccaggg ttaccgccaa
     9301 tcagaatggc acttcccggc accacgccgc cgcctagtac gcggtcgaac tctttaaatc
     9361 cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga gagtttctgg acttttgcca
     9421 ccccggcgct accggcatag ccgctgagac gctcgttacg cgccaccgtt ggcgacgcag
     9481 caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc ccctgccagc
     9541 gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct tttgccacgg
     9601 tgtacctcgt taatgctgtg ccgcccgcag gatggcgggc gagcaattac ttctgattca
     9661 ggctacctga gaggatgcag aataccccca tcaggtcagc gtgacggatg gtgacttccg
     9721 ccttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgcc gctttgatca
     9781 tcggcaggtc attggctcca tcgccaatcg ccacggtctg cgccagcggg atttcatact
     9841 cctgcgcgag gcgagtcaga gttttcgctt tgtactgcgc gtctacgatg tcgccgatca
     9901 cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg gctgtcaggc
     9961 gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc gccggaggca atcgccactt
    10021 tccagcccag cgtttccagc ttgagcacca gttgcgttaa gcctggcatc agcggcagat
    10081 tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc acgcggctgc
    10141 gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttacc tccgccacca
    10201 tctcgcccgt tccggccagt ttggcaattt catcaataca ttcaatctgg atggcggtgg
    10261 aatccatgtc catcaccagc aaacccggcg tgcgcaggtg cgggattttc cccagcgggg
    10321 cgacatccag ctgcgcttcg tgggccaggc gtgtagcccg tgcggtgagt gaacctgcca
    10381 gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccatc gccgcaccca
    10441 gtttgctctg gtattgggtc agacgctgtt tatccagccc acgaccatac agcagccagc
    10501 cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat gaaagaggca
    10561 gacccggcca taaagagaca tcttcaggca ggtcgcacca ggtaatgtta ggcattaagg
    10621 ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg tatccattgc
    10681 ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca caaaactgaa
    10741 attccggcta catcgggcag tgattgtcct gttctgtctt gccttgttag tggcactgat
    10801 gcagggggcg tcatggttta gtcaaaacca ccagcgacag cgtaatccac agctggaaga
    10861 actggcccgc accctggcgc ggcaggtgac gctgaacgtt gcaccactga tgcgtaccga
    10921 ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg aaagccgcat
    10981 tctcgacgcg ggcgtgtatg acgaacaagg cgatcttatc gcccgttctg gcgaaagcgt
    11041 cgacgtgcgt gaccggctgg cgctcgacgg gaaaaaggcg ggcggctatt ttaaccagca
    11101 gattgtcgaa ccaattgccg ggaaaaacgg accgctcggt tatctgcgcc tgacactcga
    11161 cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca ttttacgcct
    11221 gatgttgctg ctctcactgg caatcggcgt agtgctgacc cgcacgctgc tacagggtaa
    11281 acgcacccgc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg tgccggaaga
    11341 ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg ttatgtccac
    11401 attacgcctg ctcatctctg actcttacga cccgtggttt aacctggcgg tggaagagtg
    11461 tatttttcgc cagatgcccg ccacgcagcg cgttctgttt ctctggcgca atgccgacac
    11521 cgtggtgatt ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc ggatggaaga
    11581 agataacgtc cgcctggcgc ggcgcagtag cggtggcggc gcggtgttcc acgatctcgg
    11641 caatacctgt tttaccttta tggctggcaa gccggagtac gataaaacca tctccacgtc
    11701 gattgtgctc aatgcgttga acgcgcttgg cgtcagcgcc gaagcgtctg gacgtaacga
    11761 tctggtggtg aaaaccgccg aaggcgaccg caaagtctca ggctcagcct atcgcgaaac
    11821 caaagatcgc ggcttccacc acggcacctt gctgctcaat gccgacctca gccgcctggc
    11881 aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattactt cagtgcgttc
    11941 ccgcgtgacc aacctcaccg agctgttgcc ggggatcacc catgagcagg tttgcgaggc
    12001 cataaccgag gcctttttcg cccattatgg cgagcgcgtg gaagcggaaa tcatctcccc
    12061 ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt gcccgccaga gtagctggga
    12121 gtggaacttc ggtcaggctc cggcattctc gcatctgctg gatgaacgct ttagctgggg
    12181 cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc acccgcgccc aggtgtttac
    12241 cgacagcctc aaccccgcgc cgctggaagc cctcgccgga cgactgcaag gctgcctgta
    12301 ccgcgcagat atgctgcaac aggagtgcga agcgctgttg gtcgacttcc cggaacagga
    12361 aaaagagcta cgggagttat cggcgtggat ggcgggggcg gtgcggtaac tcgctgatta
    12421 aaataccaaa ggaagggaat atgatcgttc tggaaaacca ggagcgggag attatcaact
    12481 taatgttcag ccagcatatt tcctggctag ccgctgtgcg tatcaggcat aaattatcgc
    12541 ttgctgaagt aagtaaaatg ttaggtatct cgattaattc tctaaaacag atcgaaaaga
    12601 cggaacggct tagttcaaac atcaaaagca aaatggcaga aatttatggt tgtccaccag
    12661 agttgctgat atgcccttcc tggatgacgg ctgaacataa atgaatagat tcgtgaat
//
LOCUS       DAZUCE010000144        12621 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZUCE010000144 DAZUCE010000000
VERSION     DAZUCE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.144"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            48..1562
                     /gene="caiT"
                     /locus_tag="ACHY03_003426"
     CDS             48..1562
                     /gene="caiT"
                     /locus_tag="ACHY03_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFS3906618.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1593..2735
                     /gene="caiA"
                     /locus_tag="ACHY03_003427"
     CDS             1593..2735
                     /gene="caiA"
                     /locus_tag="ACHY03_003427"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFS3906619.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAALYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2864..4081
                     /gene="caiB"
                     /locus_tag="ACHY03_003428"
     CDS             2864..4081
                     /gene="caiB"
                     /locus_tag="ACHY03_003428"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFS3906620.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4155..5708
                     /gene="caiC"
                     /locus_tag="ACHY03_003429"
     CDS             4155..5708
                     /gene="caiC"
                     /locus_tag="ACHY03_003429"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFS3906621.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5817..6602
                     /gene="caiD"
                     /locus_tag="ACHY03_003430"
     CDS             5817..6602
                     /gene="caiD"
                     /locus_tag="ACHY03_003430"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFS3906622.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQLLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6608..7198
                     /gene="caiE"
                     /locus_tag="ACHY03_003431"
     CDS             6608..7198
                     /gene="caiE"
                     /locus_tag="ACHY03_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFS3906623.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQTGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7317..7712)
                     /gene="caiF"
                     /locus_tag="ACHY03_003432"
     CDS             complement(7317..7712)
                     /gene="caiF"
                     /locus_tag="ACHY03_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFS3906624.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7974..11195)
                     /gene="carB"
                     /locus_tag="ACHY03_003433"
     CDS             complement(7974..11195)
                     /gene="carB"
                     /locus_tag="ACHY03_003433"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFS3906625.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLALSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVCLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11213..12361)
                     /gene="carA"
                     /locus_tag="ACHY03_003434"
     CDS             complement(11213..12361)
                     /gene="carA"
                     /locus_tag="ACHY03_003434"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFS3906626.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2935 a         3294 c         3356 g         3036 t
ORIGIN      
        1 aaacgggaac gcaaaataaa tattcgtttt cacagtggaa ttaactcatg aagaatgaaa
       61 agagaaaaac gggaatagaa ccgaaggttt tctttccgcc gttaataatc gtcggcatac
      121 tttgttggct tacagtcaga gatctggatg cggcgaatgt cgttattaat gctgtattca
      181 gttacgtcac caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg
      241 gttggttctg gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccgccag
      301 aatttagcac cgccagttgg atctttatga tgttcgcctc ctgtacgtct gctgccgtac
      361 tgttctgggg atcgattgag atctactact acatctccac cccgccgttt ggcttagaac
      421 cgaactcgac aggagcgaaa gagttggggc tggcttacag cttgttccac tggggacctc
      481 tgccgtgggc cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca
      541 aaatggaagt gattcgcccc agctccaccc tggtgccgct ggtaggtgaa aaacacgcca
      601 aagggttgtt cggcactatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg
      661 gtaccagtct gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca
      721 ttccgcatac cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca
      781 tttgcgtcgc ctgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc
      841 tgagcttcct gatgctgggt tgggtgttca ttgtcagcgg tgccagcttc atcatgaact
      901 acttcaccga ttcggtgggg atgttgctga tgtatctgcc gcgaatgttg ttctataccg
      961 atcccatcgc taaaggcggc ttcccgcagg gctggaccgt gttctactgg gcatggtggg
     1021 tgatttacgc catccagatg agtatcttcc tcgcccgcat ctcccgtggt cgtactgtgc
     1081 gtgaactgtg cttcggcatg gtgatggggc tgacagcatc cacctggatc ctgtggaccg
     1141 tgctcggtag taacactctg ctgttgatgg ataaaaacat catcaacatt ccaaatctga
     1201 tcgaacagta cggtgtggcg cgcgccatca tcgaaacctg ggccgctctg ccactcagca
     1261 ccgccaccat gtggggcttc ttcatcctct gctttattgc caccgttacg ctggttaacg
     1321 cctgctctta taccctggcg atgtctactt gccgcgaagt acgcgatggt gaagaaccac
     1381 cgctgctggt gcgtatcggt tggtcaattc tggttggcat tatcggtatt gttctgctgg
     1441 cgctcggcgg cctgaaaccg attcaaaccg ccattatcgc cggaggatgc ccgctgttct
     1501 tcgtcaacat tatggtgacg ctctccttta ttaaagacgc gaaacagaac tggaaagatt
     1561 aattaacccc caaaatatca agaggttgaa agatggattt taatttaaat gatgagcagg
     1621 aactgtttgt cgccggtatc cgcgaactga tggccagcga aaactgggag gcctattttg
     1681 ccgagtgcga ccgtgacagc gtctacccgg aacgttttgt caaagcactg gcggatatgg
     1741 gtatcgacag tctgctgatc cctgaagagc acggtggtct ggacgcgggg tttgttactc
     1801 tcgccgccgt gtggatggag ctgggacgtc tgggggcacc aacctatgtg ctgtaccagt
     1861 tgccgggcgg gttcaatacc ttcctgcgcg aaggcacaca agagcagatc gacaagatta
     1921 tggctttccg cggcaccggt aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg
     1981 gctccgacgt gggtagcctg aaaacgactt atacccgtag aaatggtaag atttatctta
     2041 atggtagtaa gtgttttatt accagtagcg cctacacccc gtacatcgtg gtgatggcgc
     2101 gcgacggggc ttctccggac aaacctgtct acaccgaatg gtttgttgat atgagcaaac
     2161 cgggcatcaa agtgaccaaa cttgagaagc tcggtctgcg tatggatagc tgctgtgaaa
     2221 tcacctttga cgatgtggaa ctggacgaga aagacatgtt cggtcgggaa ggtaacggct
     2281 ttaaccgcgt caaagaagag ttcgaccatg aacgtttcct ggtagccctg accaactacg
     2341 gtacggcgat gtgcgccttt gaagatgcgg cgctctacgc caaccagcgc gtgcagtttg
     2401 gcgaggctat tggtcgtttc cagttgattc aggaaaaatt cgcccacatg gcgatcaaat
     2461 taaactccat gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac ggcaccatca
     2521 cctctggcga tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt gaagtggtgg
     2581 atagcgcaat gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc atcagccgct
     2641 tctggcgtga cctgcgtgta gaccgcgtct ccgggggatc tgacgaaatg cagatcctga
     2701 cgctgggtcg cgcggtgctg aagcaatacc gctaagtccg tgcgctgcct gatgcgacgc
     2761 taatgcgtct tatcaggcct acgggagcac aaatgtaggc cggataaagc gtttacgccg
     2821 catccggcag tcatgcgcta cataaatttt tcaggagcta ataatggatc atctacccat
     2881 gccgaaattc gggccgttgg ccggattgcg cgttgtcttc tccggtatcg aaatcgccgg
     2941 gccgttcgcc gggcaaatgt tcgcagaatg gggtgcggaa gttatctgga tcgagaacgt
     3001 cgcctgggcc gacaccattc gcgttcaacc gaactacccg cagctctccc gccgcaattt
     3061 gcacgcgctg tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc tgaaattaat
     3121 ggaaaccacc gatatcttca tcgaagccag taaaggtccg gcctttgccc gtcgtggcat
     3181 taccgatgaa gtactgtggc agcacaaccc gaaactggtt atcgctcacc tgtccggttt
     3241 tggtcagtac ggcaccgagg agtacaccaa tcttccggcc tataacacca tcgcccaggc
     3301 cttcagtggt tacctgattc agaacggtga tgttgaccag ccaatgcctg ccttcccgta
     3361 taccgccgat tacttttctg gcctgaccgc caccacggcg gcgctggcag cactgcataa
     3421 agcgcgtgaa accggtaaag gcgaaagtat cgacatcgcc atgtatgaag tgatgctgcg
     3481 tatgggccag tacttcatga tggattactt caacggcggc gaaatgtgcc cgcgcatgag
     3541 caaaggtaaa gatccctact acgccggttg cggtctgtat aaatgcgccg acggctacat
     3601 cgtgatggaa ctggtgggca ttacccaaat tgaagagtgc tttaaagata ttggcctcgc
     3661 acatctgctc ggtacgccgg aaatcccgga aggcactcag cttatccacc gtatcgaatg
     3721 cccttacggc ccactggtag aagagaaact cgatgcctgg ctggcggcac ataccatcgc
     3781 agaagttaaa gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac tgaccgtacc
     3841 ggaactggaa agcaatccac agtatgttgc ccgtgaatca atcactcagt ggcaaacgat
     3901 ggatggtcgc acctgcaaag ggccgaacat catgccgaaa ttcaaaaata accccggaca
     3961 aatctggcgc ggaatgccct cacatggcat ggacacggct gccattttga aaaatatcgg
     4021 ctacagcgaa aacgacattc aggagttggt cagcaaaggt ctggccaaag ttgaggacta
     4081 aacgcgtggc gtcagtcggc ttgcctgact agcgcccgcc caataacaaa cgaacaaaaa
     4141 tggatagagg tgcaatggat atcattggcg gacaacatct acgtcaaatg tgggacgatc
     4201 ttgcggacgt ttacggtcat aaaacggcgc tgatttgtga atccagcggc ggagtcgtta
     4261 accggtatag ttatcttgag ttaaatcagg agattaaccg cacggcaaac ctgttttata
     4321 cgctggggat tcgcaaaggc gacaaggttg cactacatct cgacaactgc ccggaattta
     4381 tcttttgctg gttcgggctg gcaaaaattg gcgcgattat ggtgccgatt aacgcccgcc
     4441 tgttacgcga ggaaagcgcg tggatcctgc aaaatagcca ggcgtgcctg ctggtgacca
     4501 gtgcgcaatt ttatcctatg tatcaacaga ttcagcagga agatgccact caattgcggc
     4561 acatttgcct gacagatgtg gcacttcccg ctgatgatgg cgtgagttcg tttactcaac
     4621 tgaaaaatca acaacctgcc accttgtgct atgcaccgcc gctatcgact gacgatacgg
     4681 cggaaattct cttcacctcc ggcaccacct cccgaccgaa aggtgtggtg attacccatt
     4741 acaacctgcg cttcgctgga tattactccg cctggcagtg tgcactgcgt gacgatgacg
     4801 tctacctgac ggtaatgcct gcgtttcata tcgattgcca gtgtactgcg gcgatggcgg
     4861 cgttttctgc cggggccacc tttgtgctgg tcgagaaata cagcgcccgc gccttctggg
     4921 gacaggtgca gaagtaccgc gccaccatta ccgaatgtat tccgatgatg atccgtacgt
     4981 tgatggtaca gccgccttca gcgaacgatc ggcaacaccg cctgcgggaa gtgatgtttt
     5041 atctcaactt gtcggagcag gaaaaagatg cgttttgtga acgcttcggc gttcgcttgc
     5101 tgacgtctta tgggatgacg gaaaccattg tgggcattat cggcgatcgt cctggcgata
     5161 aacgacgctg gccgtcgatt ggtcgggcgg ggttttgcta cgaagcggag atccgcgacg
     5221 atcacaatcg cccgctcccg gcaggtgaga tcggtgaaat ctgtattaaa ggcgtaccag
     5281 ggaaaaccat cttcaaagag tattttctca acccgaaagc cactgcgaaa gtgctggaag
     5341 ccgacggctg gctacatacc ggcgatacag gatactgcga cgaagaaggc tttttttatt
     5401 tcgtcgatcg ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc tgcgtggagc
     5461 tggaaaatat catcgccacc catccgaaaa ttcaggacat cgtggttgtg ggtattaaag
     5521 attcgattcg cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt gaaacattga
     5581 gcgaagagga attttttcgc ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt
     5641 atctggagat cagaaaagat ctgccacgta attgctcggg gaaaataatt agaaagaatt
     5701 tgaaataaat gaaacggcag ggaactaccc tgccagctaa taatcacgct ctaaaacaat
     5761 atgcattttt tgcaggaatg ttgtcgtccc tgaaaaagca aaaatggaga aaaggaatga
     5821 gtgaatcatt acatctgacc cgcaatggat caattcttga aattaccctt gatcgcccaa
     5881 aagcgaatgc tattgatgca aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc
     5941 gtgacgatcc gcaattacgt gtcgccatta ttaccggtgc cggagagaaa ttcttttccg
     6001 cgggctggga tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac tttggcccgg
     6061 gtggttttgc gggattaacc gaaattttca atctcgacaa accggttatc gcagctgtga
     6121 acggctacgc ctttggcggc ggtttcgaac tggcgctggc ggcagatttt attgtttgtg
     6181 ccgataacgc cagcttcgcc ctgccggaag ccaaactggg catcgttcct gacagcggcg
     6241 gtgtgctgcg tctgccgaag atcctgccgc ctgccatcgt caatgaaatg gtgatgaccg
     6301 gcagacgaat gggcgcagaa gaggcgctgc gttgggggat agtcaaccgc gtggttagcc
     6361 aggcggaact gatggataac gcccgcgaac tggctcagct gctggttaac agcgccccgc
     6421 tggcgattgc ggcgctgaaa gagatctacc gcaccaccag cgaaatgccg gtagaagaag
     6481 cgtatcgcta tattcgcagc ggcgtgttga aacactatcc atcggttctg cattcggaag
     6541 atgccattga agggccgctg gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt
     6601 aacaaccgtg agctattacg cctttgaggg gttaattccg gtggttcacc cgacggcgtt
     6661 tgtccatccc agtgcagtct tgattggcga tgtgattgtg ggagccggtg tctacattgg
     6721 cccactcgcc tcactgcgtg gtgactacgg gcggttgatc gtgcaaacgg gagccaatat
     6781 tcaggatggc tgcattatgc atggctactg cgacactgac accatcgttg gggaaaacgg
     6841 ccatatcggg cacggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg
     6901 gatgaacagc gtgattatgg atggcgcggt cattggcgaa gagagcattg ttgccgccat
     6961 gagctttgtc aaagcaggct ttcacggcga gaaacgccag ttgttgatgg gtacgccagc
     7021 ccgcgctgta cgcagtgtta gtgacgacga gttacactgg aaacggttga ataccaaaga
     7081 gtatcaggat cttgtcgggc gctgtcatgc atcgttacat gaaacacagc cactgaggca
     7141 aatggaggaa aatcgccccc gtttgcaggg gacgacggat gtgacgccta aacggtgatc
     7201 ttgccggatg cggcgtaaac gccttatcca actctgctgg agagcaaccg taggccggat
     7261 aagatgcgcc agcatcgcat ccggcgacca ggtcgcacgg tatcatcgaa atgacattaa
     7321 cgacgcatac tctttgacaa catcatctgc cacttctgct cgcggttaag ttccgtgagc
     7381 ggaacggcag gaatacgcgg tgtctttctt acgccgacta atttttcccg actgttattg
     7441 cgcaggcgcg cgtaaatttg ctcatcgata tcgaccactt taaccagtcg ctgacactgg
     7501 catccccgcc cttccagctt attagggatc atcttaactt cgcagcttat ttctgtgact
     7561 tccgacagaa tataagtcag ggtattaacc gccttgctgt gttcaatatc gaaatgaata
     7621 gagatctctc ttgctatcac ccaccgattt tcagccatca tccattcggc gattaacaag
     7681 tagagtggtt tttcaacata tccttcacac atacgaactc cagtttcaca aattaaaagt
     7741 ctggctatga ctcgttttga agcaattaaa caaggccata ttaaaattgt ggatatatcg
     7801 atagtatgca acttcaccgt cgataagtgt gagctaaatc ctgcttatgt catcaaaaat
     7861 atcgattccc caaaagattt acttaatggg caaaaaaata cggtcctcat cagagaaccg
     7921 tattagttat tcgatcaaat tagcggatga aaaatatccg ccatgacacg ctattatttg
     7981 atctgtgcgt gcatttcctg caccgaaatt actttttcag tcgcatcggc attcagcgcc
     8041 atcgcggtag cgaaaccacc gttcagggtg gtgtcataat gcactttata ttgcagcgca
     8101 ctgcgacgga tcacgcggga gtcttcaatc gcacgacggc ctgaggtggt gttgatgatg
     8161 taggtatatt cgccattctt gatacggtcc tgaatgtgcg gacgaccttc atgcaccttg
     8221 ttcaccagac gtgggttgat gcccgcttcg cccagcacaa tcgccgtgcc gtgggtcgca
     8281 tccagctcga agccctgttt cagcagtttt gccgccaggt ccaccacgcg ttctttatcg
     8341 ccttcgcgca cggaaagcag cgcacgaccg tgtttcttca tggtggagtt gctgcccagc
     8401 tgcgctttgg caaacgcttc agcgaaggtg cggcccacgc ccatgacttc cccggtagag
     8461 cgcatttctg gccctaacag cgggtcaacg cccgggaatt tattgaacgg cagcaccact
     8521 tctttcaccg agtagtacgg cgggataact tctttggtta cgccctgctc agccagcgat
     8581 ttgccagcca tcacacgcgc cgccactttt gccagcggta cgccggtggc tttggagacg
     8641 aacggaacgg tacgcgccgc acgcgggtta acttcaatca ggtagacttc gttgtttttc
     8701 accgcaaact gcacgttcat caggccgcgc acctgcaatt cgaaggccag tttctgcacc
     8761 tgctggcgca tcacatcctg aatttcctga cttaaggtgt acgctggcag cgaacatgcg
     8821 gagtcaccgg agtgcacgcc cgcctgctcg atgtgctcca taatgccgcc aatcagcacc
     8881 atttcgccgt cgcagatggc gtccacatcc acttctaccg cgtcatcaag gaagtgatcc
     8941 agcaacactg gcgcatcgtt agacacgctg accgccgtct ggaagtagcg acgcaggtca
     9001 gcttcgtcat agacgatttc catcgcccga ccgccgagaa cgtaagacgg acgcaccacc
     9061 agcgggtagc caatctcttt tgccttctca actgccattt caatagcagt aacggtggcg
     9121 ttcgccggtt gtttcagttt cagacggtca accgcatgct ggaagcgttc acggtcttcc
     9181 gcacggtcga tagcatccgg gctggtgccg ataaccggta cgccagcggc ttccagtgca
     9241 cgcgccagtt tcagcggggt ctgaccgccg tactgaacga taacgccttt cggcttctca
     9301 atacgcacga tttccagcac atcttccaga gttaccggct cgaagtagag gcgatcggaa
     9361 gtgtcgtagt cggtagagac ggtttccggg ttacagttaa ccataatggt ttcgtaaccg
     9421 tcttcacgca gcgccagcga ggcgtgtacg cagcagtagt cgaattcgat accctgaccg
     9481 atacggtttg gaccgccgcc aagcaccatg attttttcac ggtcggtaga cggattcgct
     9541 tcgcactctt cttcataagt ggagtacatg taagcggtgt cggtggcaaa ctctgccgca
     9601 caggtatcca cgcgcttgta gaccgggtgc aggtcatatt ggtcacgcag cttacggatt
     9661 tccgcttcgc gtacgcccgc cagttttgcc aagcgcgcat cggcaaagcc tttgcgtttc
     9721 agctggcgca ggaattcagc gttcaggcca gtgatgccca cttccgccac tttctcttcc
     9781 agacgcacca gttcttcaat ctgtaccagg aaccagcggt caatgttagt caggttgaag
     9841 acgccgtcca cagacaggcc cgcgcggaaa gcatcggcga tgtaccagat acgctctgcg
     9901 cctgcgtctt tcagttcgcg acggattttg gttaacgctt ccgggtcatc caggcacact
     9961 ttcgggtcga atccagtcgc accgacttcc aggccgcgca gcgctttttg cagggattcc
    10021 tgctgcgtgc gaccaatcgc catcacttcg ccaaccgatt tcatctgagt ggtcagacgg
    10081 tcgttagcac cggcgaattt ttcgaagttg aagcgaggaa ttttagtaac cacatagtcg
    10141 atggacggct cgaaggaggc cggagtacgt ccgccagtga tgtcgttcat cagttcgtcg
    10201 agggtgtaac ccaccgccag tttcgccgcc actttagcaa tcgggaaacc ggtcgctttc
    10261 gacgccagcg ccgaagaacg ggacacgcgc gggttcattt cgataacaat caggcgaccg
    10321 tttttcgggt tcaccgcaaa ctggacgttg gaacccccgg tttcaacgcc gatttcacgc
    10381 agcaccgcca tcgaggcgtt acgcatgatt tgatattctt tgtcggtcag cgtttgggct
    10441 ggcgcgacag tgatggagtc accggtgtgg atgcccatcg catcgaagtt ttcgatagag
    10501 cagacgatga tgcagttgtc gtttttatca cgcaccactt ccatctcgta ctctttccag
    10561 ccgatcagcg actcatcaat cagcaactct ttggtcggag agagagccag accgcgggcg
    10621 caaatttctt caaactcttc gcggttataa gcgataccgc cgccgctacc gcccatggta
    10681 aaggatgggc gaataatgca cgggaagccc acgtcagcgg caaccgccag cgcttcttcc
    10741 atcgtgtgtg cgataccgga acgcgcggtt tccagaccaa ttttcttcat cgctacgtcg
    10801 aaacgacggc ggtcttctgc tttatcaatc gcatcggcag tggcaccaat catggtgacg
    10861 ccgaactctt ccaacacgcc ctgacgttcc agctccagcg cgcagttcag cgccgtctga
    10921 ccgcccatcg ttggcagcac cgcgtccggg cgctcttttt caataatctt gcgtaccact
    10981 tcccagtgaa tcggctcgat gtaggtcgca tcggccattt ccgggtcggt catgatggtc
    11041 gccgggttgg agttcaccag aatgacgcgg taaccctctt cgcgcagggc tttacatgct
    11101 tgcgcgccag agtagtcaaa ctcacacgcc tgaccgataa caatcgggcc cgcacccaga
    11161 atcaggatac tttttatatc tgtacgtttt ggcatggctc ttttactcct gattacttag
    11221 cggttttacg gtactgctca attaactcga taaagtggtc gaacaacggc gcggcgtcgt
    11281 gtggaccagg gctggcttca gggtgaccct ggaagctgaa cgccggttta tcggtgcgat
    11341 gaatgccctg taacgtaccg tcgaacaggg atttatgcgt gacacgcagg tttgcaggta
    11401 atgttgcttc atccaccgca aaaccgtggt tctgggcggt gatcatcacc acgtttttct
    11461 caacatcttt aaccggatgg ttgccgccgt ggtgaccaaa tttcattttg acagtcttcg
    11521 caccgctcgc cagcgccagc agctgatgac cgagacagat gccgaagacc ggaatatcgg
    11581 tttcgaggaa tttctggatg gcggtaatgg cgtaatcgca cggggccggg tcgccaggac
    11641 cgttggagag gaagatgccg tctggattca ttttcagcac atcttccgca gaagtttgcg
    11701 ccggaacgat ggtcaggcga cagcctctat ccaccagcat ccgcaggatg ttgcgcttgg
    11761 caccaaaatc ataagccacg acgtggaacg gcagctcgtc ttcttttttc gcttctggca
    11821 ggccgccggt caacgtccag ctcccttgtg tccagctata ggcttctgcg gtggtcactt
    11881 cttttgccag atccatgcca ttcagacctg ggaacgcgcg ggctttttct aacgccagcg
    11941 ccgcatccgg gttatcgcct gcgataatgc agccattctg tgcgcctttc tcgcgcagta
    12001 aacgcgtcag cttacgagta tcgatatcgg caatcgccac gatgttatgg cgcttcaggt
    12061 aagaagagag gtcttcggta ttacggaagt tgctggcaat cagcggcagg tcgcgaatca
    12121 ccagaccttg tgcatgtacc tgagaagatt cttcatcggc gtcattagtg ccgacattgc
    12181 caatatgggg ataagtaaga gtaacgattt gacgagaata ggaaggatca gtgaggattt
    12241 cttgataacc ggtcattgaa gtattgaaaa cgacttcccc aaccgccgaa cctgttgccc
    12301 ctatggcccg accgtgaaac tgggttccgt cttccagaac caatagcgct gacttaatca
    12361 aaacaccctc cagagaatat tcactcactt tatttgcata ttaattcaca atgatgacgt
    12421 aaatcaatgc aaatctgctt accgacgaat ttctggcaaa cggcggcatt ctggagatat
    12481 gggcgctaaa agtcaactta atggcaggat tttttatatt attttgtggc tcatgcatgt
    12541 tttacaggca ttaagcgaca aaaagatcat ctaacctgtt ctggaaaacg cttgcgcagc
    12601 aagaagattg catgaaataa a
//
LOCUS       DAZUCE010000145        12620 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZUCE010000145 DAZUCE010000000
VERSION     DAZUCE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.145"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            279..2429
                     /gene="btsT"
                     /locus_tag="ACHY03_003435"
     CDS             279..2429
                     /gene="btsT"
                     /locus_tag="ACHY03_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFS3906627.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2479..2682
                     /locus_tag="ACHY03_003436"
     CDS             2479..2682
                     /locus_tag="ACHY03_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFS3906628.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2693..3649
                     /gene="yjiA"
                     /locus_tag="ACHY03_003437"
     CDS             2693..3649
                     /gene="yjiA"
                     /locus_tag="ACHY03_003437"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFS3906629.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3683..3985)
                     /locus_tag="ACHY03_003438"
     CDS             complement(3683..3985)
                     /locus_tag="ACHY03_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFS3906630.1"
                     /translation="MSMVKHKRGAPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(3972..4259)
                     /locus_tag="ACHY03_003439"
     CDS             complement(3972..4259)
                     /locus_tag="ACHY03_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFS3906631.1"
                     /translation="MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIEN
                     GEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIISARKADRKERSRYEHG"
     gene            4456..5622
                     /locus_tag="ACHY03_003440"
     CDS             4456..5622
                     /locus_tag="ACHY03_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS3906632.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASDRSAKSTLYSRLYNLIDRDMVTREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            5686..7305
                     /locus_tag="ACHY03_003441"
     CDS             5686..7305
                     /locus_tag="ACHY03_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFS3906633.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPANTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTEDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTTEDIATIADTYHAWRSTPEELT
                     TRIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRKNLEALGYGE"
     gene            7295..8674
                     /locus_tag="ACHY03_003442"
     CDS             7295..8674
                     /locus_tag="ACHY03_003442"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFS3906634.1"
                     /translation="MGNKWPEFRLDEITTLIVDCPHSTPEWTDSGVIVLRSNNIRNGA
                     LDLATPSFTTEEGYLERIKRAIPSEEDIVLTREAPMGEVCIIPKGIKCCLGQRMVLIR
                     ADKSQVIPEYLLYVMQSSYIQHQISWNEGTGTTVSNIRIPNIKALKIPLPKITIQRTI
                     VSNLLVIDKKIAINTGINQTLEQMSQTLFKSWFVDFDPVVDNALDAGNPIPEALQSRA
                     ELRQKVRNSTDFKPLPAEIRSLFPSEFEETELGWVPKGWTLKSVAKSININPSIKLPK
                     NKIAKYVDMKSLPTQGYSISDIIEKPYSGGAKFQNNDTLFARITPCLENGKTGFVDFL
                     DEKETAFGSTEFIVMRGTPQVHYLYVACLARESNFRLHAIQNMVGSSGRQRVQNSCFD
                     SFYIAIPTPAVMSLFSGKVSSYFDKMYFCNLENKSLTTLRDTLLPKLISGELSLDDLP
                     DLTTDTEAA"
     gene            8769..12032
                     /locus_tag="ACHY03_003443"
     CDS             8769..12032
                     /locus_tag="ACHY03_003443"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFS3906635.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYMNPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEASIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEAEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYDFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDYIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            12247..12585
                     /gene="symE"
                     /locus_tag="ACHY03_003444"
     CDS             12247..12585
                     /gene="symE"
                     /locus_tag="ACHY03_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFS3906636.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESKLMQSLRQVCKLSARKQKQVQDF
                     IGVISSKTPR"
BASE COUNT         3376 a         3103 c         3072 g         3069 t
ORIGIN      
        1 atataatttt taaatgagtt acacaaccga gggctaaaaa tcgcttcggt tgtgatcatc
       61 atcacataaa taaccttcat aaaccttgct caaaaccgct caaccgcaaa actgaccgct
      121 tacgtcccta aaataaccac tcagttattt accttacttt acgcgcgcgt aactctggca
      181 gcatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac ctagaacggc
      241 ttcggccaac tattaatcaa tacatgccag gttttactat ggatacgaaa aaactattca
      301 agcacatacc ctgggtgatt ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat
      361 tacgtcgggg ggagcacgtc agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc
      421 tggtggcgta tcgctactac agtttgtata tcgcccagaa ggtgatgaaa ctcgacccca
      481 cgcgcgcgac gcctgcggtt attaacaacg acggtctgaa ctacgttccg accaaccgtt
      541 acgtgttgtt tggtcaccac ttcgccgcta tcgccggtgc tggtccgctg gttggtccgg
      601 ttctcgccgc gcagatgggc tacctgcctg gcacactgtg gctgctggcg ggggtagtgc
      661 tggccggtgc ggtacaggac tttatggtgc tgtttatctc ctctcgccgt aacggagcat
      721 ctctcggtga gatgatcaaa gaagagatgg ggccagtacc ggggactatc gcgctgtttg
      781 gctgtttctt aatcatgatc atcatccttg ccgtcctggc gctgattgtg gttaaagccc
      841 tggccgaaag tccgtggggt gtcttcaccg tttgctcaac cgtaccaatt gcgctgttta
      901 tgggtatcta catgcgcttt atccgtccgg ggcgtgtggg tgaagtctct gtcattggta
      961 tcgtgctgct ggttgcctct atctacttcg gtggcgtgat tgctcacgat ccgtactggg
     1021 gtccggcact gacctttaaa gacaccacca ttaccttcgc gctgattggc tatgcgtttg
     1081 tttccgcgct gctgccggtg tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc
     1141 tgaaaatcgg cgttatcgtc ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga
     1201 aaatgcctgc gatgacccag tacattgacg gtactggccc gctgtggaaa ggcgctctgt
     1261 tcccgttcct gttcatcacc atcgcctgtg gtgcggtatc tggcttccac gcgctgatct
     1321 cttccggtac gacgccgaaa ctgctggcta acgaaaccga cgcgcgtttc attggctacg
     1381 gcgcaatgct gatggagtcc ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg
     1441 aaccgggtct ttacttcgcg atgaataccc cgcctgcggg ccttggcatc accatgccta
     1501 acctgcatga aatgggtggc gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta
     1561 ccgcacacgc ggcagcgacc gtcagctcct ggggcttcgt gatttcgcca gagcagatcc
     1621 tgcaaaccgc gaaagacatt ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccga
     1681 cgctggcggt aggtatcgct cacgtgttcc acaaagtgct gccgatggct gacatgggct
     1741 tctggtatca cttcggtatt ctgttcgaag ccctattcat cctgaccgcg ctggatgcgg
     1801 gtacccgttc tggccgcttt atgttgcaag acctgctggg taacttcatc ccgttcctga
     1861 aaaaaaccga ctctctggtt gccggtatca tcggtactgc gggctgtgtg ggtctgtggg
     1921 gctacctgct gtatcagggc gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc
     1981 tgttcggtat ctccaaccag atgctggcag ccgtggcgct ggtactgggc accgttgtgc
     2041 tgattaagat gaagcgcacc caatacatct gggttactgt tgttccggct gtatggctgc
     2101 ttatctgcac cacctgggcg ctgggcctga aactattcag caccaacccg cagatggaag
     2161 gcttcttcta catggcaagc cagtacaaag agaagattgc taacggtact gacctgacgg
     2221 cgcagcagat tgccaacatg aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga
     2281 gtattctgtt cctgattgtg gtgtacagca tcatcttcta cggtttcaaa acctggcttg
     2341 cggtgcgtaa cagcgacaaa cgtactgaca aagaaacacc gtacgttcca atcccggaag
     2401 gcggcgtgaa gatctcttcg caccactaac cgtgtttagc cccgcttcgg cggggctttg
     2461 ttcttatcag agtgaactat gtttggtaac ttaggacagg caaaaaaata tctcggccag
     2521 gcggcgaaga tgttgattgg cattccagac tacgacaact acgtcgagca tatgaagacc
     2581 aaccatcccg acaagccgta catgagctat gaagaattct tccgcgaacg ccagaatgca
     2641 cgctacggcg gcgatggtaa aggcggtatg cgctgttgtt aatggagaga gcatgaaccc
     2701 gattgcagtt accctactca ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca
     2761 tattcttaat gaacaacacg gctacaagat tgccgtgatt gaaaacgaat tcggcgaagt
     2821 ctccgttgat gatcaactaa ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg
     2881 ctgcatctgc tgttcgcgct ccaacgagct ggaagacgca ctactcgacc tgctggataa
     2941 tctcgacaag ggcaatattc agttcgaccg tctggtcatt gaatgcaccg gcatggccga
     3001 tcccggcccg attattcaga cctttttctc ccatgagatt ttatgccagc gttacctgct
     3061 ggacggcgtg attgcgctgg tggatgcggt acatgccgat gagcagatga accagttcac
     3121 catcgcccag tcccaggtgg gctacgccga tcgtattctg ctgaccaaaa ccgacgtcgc
     3181 aggcgaagca gaaaaactgc gtgaacgcct ggcgcgcatc aacgcccgtg caccggtcta
     3241 caccgtcacc cacggcgaca tcgacctggg cctgctgttc aacaccaacg gttttatgct
     3301 ggaagaaaac gtcgtcagca ccaaaccgcg tttccacttt atcgcggata aacaaaacga
     3361 tatttcgtcg attgtggtgg aactggatta cccggtagat atcagcgaag tttcccgggt
     3421 gatggaaaac ctgctgctgg aatcggcaga taaactgctg cgttacaaag ggatgctatg
     3481 gattgacggc gaacctaacc gcctgctatt ccagggcgtc cagcgcctct acagcgccga
     3541 ctgggaccgc ccgtggggcg atgaaaaacc gcatagcacg atggtgttta tcgggattca
     3601 gctgccggag gaagagattc gggcggcgtt tgcggggttg aggaagtaat tttggatgat
     3661 ggccgccatg tggcggcctg aattattttt tgttctgatc gcgcagcatc gcttcacgta
     3721 aaattgcgtt caggcgagtt tgatagcctt tacctggtcg cttcagccat tccatcacat
     3781 cagcatcaat acgcaccgaa gcctgagttt tcaatggccg ataaaactta ccgcgttctg
     3841 cgttagacca ttgctcatcg ctggaaggcg ggatatcact gtaatcaata tcttcgtcgg
     3901 atttattcgc cagtgccttc agttcggcct catgctgcac atcgagtgta gagggtgccc
     3961 ctcgtttatg tttaaccatg ctcatagcga ctcctctctt tacgatctgc ttttcgtgca
     4021 ctgatgattc gaataatttc atttccgctt tcgaaacgga tggtatgcgc aaccagaata
     4081 accacgatac cgtgaaccaa gccgatagtc tgccagcgat actcaccgtt ttctatgcga
     4141 tcttgctgtg acaaatgttg tggatcatca aacactaaca ccgcatcttc aaagcggatg
     4201 ccatgtttca cccgattact tttggccttg tttgcatccc attcaaactc cattggcata
     4261 gttgccatcc ctaattgtat atacaaaaat gtatctacaa ttattctgca attatagcgt
     4321 tgatgttgga aatagcatca cgttttggat aatcaaccat gaaattgaca atgagtaagt
     4381 tggacttgat tcatccaata gaatttctcg cctgacatgg cacactaaac acctcgctgt
     4441 cgttaaggga ttaccgtgaa aaactacaaa gttattaccc cgctttttcc tacctatgca
     4501 caggttaaag ctatgatgaa agccgtctca ggctattccc tgaaagcggt acgcaatatg
     4561 attaccgcca ttcacgagca aaccggtacg ccgcaaaaac ccgttgactg gtcggaaccc
     4621 gacctgtgga tcagcgagcg cttaacgggt gaagatgccg acatcgcccg tcgtatttgg
     4681 gataccgata atcacatatt gaacccgcgc cacagctacg gctgctactt attcctcaac
     4741 tatccccagt ttgagctgat ggaaagcacg ccagatgata cctggcagcc cacctcgcgc
     4801 gggcaaaaat tcctgcaaga tgacgaaaaa acgctgcgtt cactggacga ccaggaaggc
     4861 attctgcaac tgcttgagct gctggctggc cgggaaatgt cgcgccgcgc cgatttatta
     4921 ccggaatggc aggccttttt gcatcagcat tccaaattcg cttccgaccg gtcagccaaa
     4981 agtacgctct actcccgtct ctataacctt atcgaccgcg acatggttac tcgcgaaggc
     5041 atgagctatc gcatcaccga taccgggcgc gcctggcttg gaaaagcgtt gccagcacga
     5101 aaaagcgatc cgcgtaaaga gctgctggaa gcggtgaaac gctacaacga gcagcaaaaa
     5161 gagctgctgc gcgaacaaat cagcacaatg aacccgtaca aatttgagca actggtcggt
     5221 caattactgg aagcaatggg ctacgagcag gtcgaagtga ccaaagcctc aggcgataaa
     5281 ggtgtggacg tgattggtca ggtgcaggtc ggcatcacca ctattaccga agtggtgcag
     5341 gtcaaaagaa tgcagaacac catcactcga ccgtatatcg accagttgcg cggagcgttg
     5401 ccttaccaca aggccattcg cggtacgctt atcaccaccg ggaaatttgc ggcgaaatgt
     5461 gcagaagcgg cgctctttcc gggggcagcg cccattacgc ttatcgacgg cgatcgctta
     5521 ctggaactgt taatcgaaaa taacgtcggc attcgccgca gcaatgcagt ggaattgctg
     5581 gatgtggatt tgcagttgtt tgatgagctg gagatggatt aaagggcgaa atccgccgcc
     5641 tcaaccatta caatagcgac ccaatttatc tgaacagagt tcatcatggc caaagcacca
     5701 actaaaaaag ccaaagcaaa gaaaggcttt gaagaaacat tatgggatac cgcaaaccag
     5761 cttcgcggta gcgttgagtc ctccgaatac aagcacgtgg tgttgagcct cgtattcctg
     5821 aaattcatca gcgataagtt tgaagcacgc cgcaaaaaaa tgattgccga tgggcaagca
     5881 gacttccttg agatggaagt gttctaccag caggacaaca ttttctacct gccggaagag
     5941 gcgcgctggt cgtttatcaa acaaaatgcc aaacaggacg atattgcggt ccgtattgac
     6001 accgccctct cgaccattga gaaacgtaac ccaaccctga aaggtgcgct gccagacaac
     6061 tacttcagcc gtcagaatct ggaaaccaaa aaactggcgt cactgattga taccatcgac
     6121 aacatcgaaa cgctggcaca cgaaactgac gttgaaacgt tatcgaaaga agacctggtc
     6181 ggacgcgttt acgaatactt cctcggtaag tttgccgcaa ctgaaggcaa aggcggtgga
     6241 gagttctaca cgcccaaatg tgtggtcacg ctgttaactg aaatgctcga acccttccag
     6301 ggcaaaattt atgacccgtg ctgcggctcg gcagggatgt tcgtgcagtc ggtgaagttc
     6361 gtcgagagcc atcagggtaa aagccgtgat atcgcgctgt atggtcagga actgacagcc
     6421 acgacgtata aactggcaaa aatgaacctc gccattcgcg gtctttcagc taacctcggc
     6481 gaacgccctg caaacacttt ctttagcgac cagcacccgg acctgaaagc cgactacatt
     6541 ctggcgaacc cgccgttcaa cctgaaagac tggcgcaatg aagccgaatt aaccgaagat
     6601 ccacgctttg ccggttatcg tatgccgcca accggtaacg ccaactacgg ctggattttg
     6661 catatgctct ccaagctgtc ggctaacggc acagcaggtt ttgtgttggc aaacggttcg
     6721 atgagttcta acaccagcgg cgaaggagag atccgtgcac agatgatcga aaatgatcta
     6781 atcgactgca tgatcgctct gccaggtcag ttgttttaca ccacgcaaat cccggtgtgt
     6841 ttatggttta tgaccaaatc gaaggctgcc gatccggcca aaggttatcg tgatcgtcag
     6901 ggcgagacgc tgtttattga tgcgcgtaac ctcggcacca tgattagccg caccaccaaa
     6961 gagctgacga cagaggatat cgccaccatc gccgatacct accatgcatg gcgcagtacg
     7021 ccggaagaac tgactacgcg gattgcgcgt ggtgacagca agctggaaaa atatgaagac
     7081 caggctggct tctgcaaagt tgcaaccctg caagacatta aagataacga ctacgttctg
     7141 acaccgggcc gctatgtggg tgcagccgag caggaagaag acggcgtggc gtttgagacg
     7201 aaaatgcgcg aattgtcgaa gacgttgttt gagcagatga agcaggcgga agaactggat
     7261 cgtgcgattc gcaagaattt ggaggcgctg ggttatgggg aataagtggc cagaattccg
     7321 tttggatgaa attacgactt taattgttga ttgtcctcat tcaacgccag aatggacaga
     7381 ctcaggagtt attgttctca gaagtaataa tatccgcaat ggtgctttag atttagcaac
     7441 ccctagtttt acaacagaag aaggttatct agaacgtatc aagagagcca ttccctctga
     7501 agaagatata gttctaacca gagaagcgcc aatgggtgaa gtatgtataa ttccaaaagg
     7561 gataaaatgc tgcctgggac aaagaatggt attaatacgt gcggataaat ctcaagtcat
     7621 cccagaatat ctactatatg taatgcagag ttcatatatt caacatcaaa ttagctggaa
     7681 tgaaggtact ggaacaaccg taagcaatat cagaatacct aatattaaag cattaaaaat
     7741 acctttacca aaaatcacta ttcaacggac aattgtttct aatttattag tgattgacaa
     7801 aaaaatagca ataaatactg ggatcaacca aaccctggaa caaatgtccc aaaccctgtt
     7861 caaatcttgg tttgtggatt tcgatccggt ggttgataac gctctggatg caggaaatcc
     7921 aatcccggaa gctctgcaat ctcgtgccga gttgcgtcaa aaagtacgta atagtacaga
     7981 tttcaaacca cttccggcgg aaatccgctc gcttttccca agtgaatttg aagaaacgga
     8041 gttgggttgg gtgccaaagg gctggacgct gaagtctgta gcaaaatcta taaatataaa
     8101 tcctagtata aaattaccaa aaaataaaat cgcaaagtat gttgatatga aatctttgcc
     8161 tactcaagga tattcaatat cagatattat agaaaagcca tattcagggg gagcgaaatt
     8221 ccaaaataac gatactttat tcgctcggat aactccatgc ctagaaaatg gtaaaacagg
     8281 atttgttgat tttttagatg aaaaagagac tgcgtttggt tctactgaat ttatagtgat
     8341 gcgaggtact cctcaagtac attatttgta tgtagcttgt ttagcccgag aaagtaattt
     8401 caggttacat gcaatacaaa acatggtcgg ttcatccggc cgccaacggg tacagaacag
     8461 ctgctttgat agcttttata ttgcaatacc aacacctgct gtaatgagct tattttcagg
     8521 aaaagtcagt agttactttg ataaaatgta tttctgtaat ttggaaaaca aatccctgac
     8581 gaccctccgc gacaccttgc tcccaaaact catctccggt gagctatccc tggatgatct
     8641 tccggatctc actaccgata cagaagctgc ataacgcatt ttgcccctgt aaaatcaggg
     8701 gctttctggt aaggttttct actgatacgg gaatgcttac cagaaattag ccagggttgg
     8761 agcgcgatat gagtctctct ttcagtgaag caaaattaga acaagcgatc attgaactgc
     8821 tacaggatca gggatatcaa cacctgatcg gcgataatgt cccacgttcg agtctcgatc
     8881 aggtcattat cgaagacgat ctccgtcatt atttagcggc acgctaccag cctgatggca
     8941 ttactgaaga agagattcag cgactgatca aacagttcac cacgcttccg gcctccgacc
     9001 tttatgaaag caacaaaaca ttttgtcgct ggctggcaaa tggttttctg ttcaaacgcg
     9061 acgatcggca acaaaaagat ctctatattg aattgctcga cacccggcat ctacctgccg
     9121 cactgcgcca gatatttgac gccgaagatg tcccgttgca acaggcggcg gaactcccgc
     9181 cttcctatat gaatccgccg cttaaccgga ttaagattgt taaccagctt aaaatctccg
     9241 gcaaagataa tcagactcgt attcctgacg gcattctcta tatcaatggt ctgccattgg
     9301 tcgtctttga atttaaaagt gcggtgcgcg agcaggaagc cagtattggc gatgcctgga
     9361 agcagctctg taaacgctat cgccgggata ttccgcaact gtttatctac aacgcgctct
     9421 gcattattag cgatggcgta aataaccgga tgggcaacct gtttgcgccc tatgaatact
     9481 tttactcatg gcgcaaagtc accggtaatg aaaaccgtga acaggacgga attccatcat
     9541 tgcactcaat gattcagggg ctgtttcatc cggtacgtct gctggatgtg attaaaaact
     9601 ttatctgctt cccggataaa gccaagcacg aagtaaaaat ttgctgccga tatccgcagt
     9661 actatgccgc ccgcaaactc tattacagca tcaagcaagc gcgtaaacct ttcggtagcg
     9721 gtaaaggcgg cacctacttt ggcgcaacgg gctgtggcaa aagttacacc atgcaatttt
     9781 taacgcgtct tttgatgaag agcgtggagt ttgccagccc gaccattgtt ttgatcaccg
     9841 accgcaccga tctggacgat cagctttctg cgcaaatgtg caacgccaaa aattacattg
     9901 gcgacgacac catcgttcct gttaccagcc gtgaagattt gcgtaatcaa ctggcgggac
     9961 gcaatagtgg cggtgtattc ctgacaacga tccataaatt caccgaagac accgaactcc
    10021 tttctgaacg cagcaatatc atttgcatct cggacgaagc acatcgcagc caggttaacc
    10081 tcgaccagaa agtcatcgtc gataaagaaa gcggaaaagt gcgcaaaact tatggctttg
    10141 cgaaatacct gcacgattca ctgccaaacg ccacctatgt tggctttacc ggcacaccga
    10201 ttgacgcgac gctcgatgtc ttcggtgagg tgatcgacag ttacaccatg accgaatccg
    10261 ttcaggatga aatcactgta cgcatcgtgt acgaaggccg tgcggctaaa gtgatcctgg
    10321 actccagcaa actggaagaa gccgaaaagt attacgaaga gtgcgcaaac gcaggcacca
    10381 atgagtggca aatcgacgaa agcaaaaaag ccaccgcaac catgaatgcg gtactgggtg
    10441 atgaagatcg gttaaaagcc ctcgcggaag attttgccaa acattatgaa aaacgcgtag
    10501 ccgaaggttc caccgtaaaa ggcaaagcca tgtttgtttg tgccagccgt gaaattgcct
    10561 gggatttcta ccgtcagctt aaagctattc gccctgcctg gtttgaagtg aagcaagcac
    10621 ccgatggcgt cgtcctgacg gaacaggagc agaaagagtt accgccttca gaaatggtga
    10681 agatggtcat gacgcgcggt aaagatgacg acgaggcgct ttatgattta ctgggcacaa
    10741 aagaatatcg taaagagctg gataagcagt tcaaaaacgc taaatcgaat ttcaaaattg
    10801 ccattgttgt tgatatgtgg ctgaccggtt tcgacgtccc ggaactggat accatctata
    10861 ttgataaacc cttacaaaaa cataatctta tccagaccat ttctcgcgtt aaccgtaaac
    10921 tggaaggcaa aagtaaaggg ttagtggtgg actacatcgg cattaagagt cagatgaacc
    10981 aggcactggc aatgtattcc cgcattgatg ccaccaactt tgaagatatt cagcaatcgg
    11041 taatagaagt taaaaaccat ctcgatttgt tggggcaagt cttttacgac tttgacagtc
    11101 gggattattt tagtggtgag ccacaagcgc aattatcctg cctcaaccgc gcggcggaat
    11161 ttgttctgcg tacccagaaa gttgaacgtc gttttatggg actggttaaa cgcatgaaag
    11221 ccgcctacga cgtctgctgc ggcagcgaag cattatcaca gacagaacgt gactatattc
    11281 actattatct tgctgttcgt tcaattgttt tcaaactgac gaaaggtgac gcaccggatg
    11341 tcacccagat gaatgcacgc gttcgtgaaa tgattgcaga agcgttaaaa gctgatggcg
    11401 tagaagaaat ttattttctt ggcgataaaa aagcggaatc tatcgatatt tttgacgaag
    11461 attatctggc acgaattaac aagatcaaac ttccggcaac gaagatccag ttattacaaa
    11521 aattactgga aaaagcgatc agcgacttca agaaagtgaa ccagttgcaa gggattaact
    11581 tcacccgccg cttccaggca attatagatc gttataatga gcggcgagaa gatgatgtac
    11641 tcaacggtga agaattcgat acattcagtc aggaaatgac cgatattatc tatgatatta
    11701 aaaccgaaat gggcacctgg gccgatttag gcattgatat tgaagaaaaa gcgtttttcg
    11761 acattcttgc tcatatgcgc gataaatatc agttcaccta tgacgatgaa aaaatgctgt
    11821 cactggcaaa agagatgaaa agcgtggttg acaacacatc gaaatatcct gactggagta
    11881 aacgtgatga tattaaagcg aaactgaaag ttgaacttat tctgcttcta cacaagcata
    11941 agttcccgcc agtagcgaat gatgatgttt atatgggtgt gctggcacag gcagagaatt
    12001 ttaaagaaca ccatatgagt gctttaaatt aattatgaag gccggataca ctttgttatc
    12061 cggccattct gaattaacgt taatttctct tccagatctt tccagtaacg cttttattat
    12121 tttctatttt tagctggact ttccctgtat ttactgatga tatatccagg tatttagcgc
    12181 ggtgcggatg tgcgccaaca cacccgcacc gctaatcaca atcactattt tcaaggaata
    12241 gcagttatga ctgacacgca ttctattgca caaccgttcg aagcagaagt ctccccggca
    12301 aataaccgtc aattaaccgt gagttatgcg agtcgctacc cggattacag ccgtattccc
    12361 gccatcaccc tgaaaggtca gtggctcgaa gccgccggtt ttgccaccgg cacggcggta
    12421 gatgtcaaag tgatggaagg ctgtattgtc ctcaccgccc aaccacccgc cgccgaagag
    12481 agcaaactaa tgcagtcgct gcgccaggtg tgcaaactgt cggcgcgtaa acaaaagcag
    12541 gtgcaggatt ttattggggt gattagtagc aaaacgccac gctaagtttc tctccccttc
    12601 cccatacggg aaggggaatt
//
LOCUS       DAZUCE010000146        12525 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZUCE010000146 DAZUCE010000000
VERSION     DAZUCE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.146"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..328
                     /locus_tag="ACHY03_003445"
     CDS             <1..328
                     /locus_tag="ACHY03_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906637.1"
                     /translation="LKEIAERISKICKKHKIDFVFSFSVLTEVGNNEYKDSRFVSFGL
                     YCETTSPYIHAASEIVDSHIDPQRIQELTLALQLLRENGECNCSDCQHEKKKTTHKTT
                     NQATLH"
     gene            437..2488
                     /locus_tag="ACHY03_003446"
     CDS             437..2488
                     /locus_tag="ACHY03_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFS3906638.1"
                     /translation="MSADKQTFALHCEAKNDKVRKRLGIKGGFFWADTRKLSVAVSRC
                     IAAMDDAGYDEDDFKKPVRVNFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMMAIPFD
                     DNINGEDATTAGDDYDNLDGTIPDDVEKSESPDSDDDCSECEIPVATLSLTHRFLHLF
                     FFFSKDEDGKYRHHATPEQRNNVIRMEMDTEDSYLQSLLTAVRSHPELDKLTNYHLGR
                     LAQSVGKAFSHATPRHISPAEFDKFISTWMKTDYVDQGLLAKEWQKGNYVTGITRTPS
                     GANAGGGNLTDRGEGFTHNQASLARDIATGVLARSMDVDIYNLHPAHAKRVEEIVAEN
                     KPPFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEAIPARVTEYLNKVLTETDHSN
                     PDPEIVEIACGRSSAPMPQRGTAEEIHDDEEKQQTSDAMSNEQAAPESVEEIPVKHNA
                     DTQSLENVSSVETKYQELRAELYEARKNILPKNPVDADKLLAASRGEFVEGISDPTDP
                     KWVKGIQTRDTEDQNQSKVEQNSPDTQQNGPEEQQPGPVMQQEVEKVCTACGQNSGGH
                     CPDCGPVMGDETYAETFGENETAAGDDQAQMKNEYIQKNAVDTTQEHDSVVHIDPDSD
                     ASDNNDDSEQADFDWKRQLVLAAVYGLCANPACVATAPAIPDIAIMIANRLENFGGDK
                     S"
     gene            2485..2781
                     /locus_tag="ACHY03_003447"
     CDS             2485..2781
                     /locus_tag="ACHY03_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFS3906639.1"
                     /translation="MNAWLIPDRIEEQSWARHYRQITREENETELADDLEKGLPQHLF
                     ESLCIDNLQRHGASKKAIARAFDDDVDFQERMAEHIRYMVETIARHQIYIDSEV"
     gene            2787..3572
                     /gene="bet"
                     /locus_tag="ACHY03_003448"
     CDS             2787..3572
                     /gene="bet"
                     /locus_tag="ACHY03_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFS3906640.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQNDE
                     SCTCRIYRKDRNHPICVTEWMDECRRAPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTTERQPERDITPVNEETMSEINALLTSMEKTWDD
                     DLLPLCSQIFRRYIRASSELSQAEAEKVLGFLKQKATEQKVAA"
     gene            3569..4249
                     /locus_tag="ACHY03_003449"
     CDS             3569..4249
                     /locus_tag="ACHY03_003449"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFS3906641.1"
                     /translation="MTPEIILQRTGIDVRGVEQGDYAWQKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQF
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIS
                     FVFGEQWR"
     gene            4297..4548
                     /locus_tag="ACHY03_003450"
     CDS             4297..4548
                     /locus_tag="ACHY03_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS3906642.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERVDHNELHMIKAEIEKEMHIASLRKMRRTSIN"
     gene            4583..4678
                     /locus_tag="ACHY03_003451"
                     /pseudo
     CDS             4583..4678
                     /locus_tag="ACHY03_003451"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4810..5973
                     /locus_tag="ACHY03_003452"
     CDS             4810..5973
                     /locus_tag="ACHY03_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3906643.1"
                     /translation="MSLFRRGEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVDRLGDMPDVTFEEACLRWLEEKADKKSIDSDKSRIAFWIEHFEGIRIKDISEAM
                     IYSVISKAYNRKTKERWKLQVEAALRKGKEPPAYIPKSVSTQTKATHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVKNKRVRWLEKEEARRLIDACSDPLKSVVKFALATGLRRSN
                     IINLEWQQIDMQRRVAWVNPEDSKSNRAIGVALNDTACKVLRDQIGKHHRWVFVYTTA
                     ARRPDGTMTPSIRKMRLDYNTSWLTACRRAGIENFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDILSDDVPNLSHPEVFEDAKKA"
     gene            6014..6565
                     /locus_tag="ACHY03_003453"
     CDS             6014..6565
                     /locus_tag="ACHY03_003453"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFS3906644.1"
                     /translation="MSNNFKDLGPEVSFRRIYEYAGGDWQEDNGVWHQNVFAYYLSIS
                     CNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGCRYCHMACPYGAPQYNAEKGHMT
                     KCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQKHGTLAAVAPLPRAHFTKPNIV
                     IKPNANSRPTGDTTGYLANPEEV"
     gene            6567..7421
                     /gene="ynfH"
                     /locus_tag="ACHY03_003454"
     CDS             6567..7421
                     /gene="ynfH"
                     /locus_tag="ACHY03_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFS3906645.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            7464..8078
                     /gene="dmsD"
                     /locus_tag="ACHY03_003455"
     CDS             7464..8078
                     /gene="dmsD"
                     /locus_tag="ACHY03_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFS3906646.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKSLFR"
     gene            8237..9529
                     /gene="clcB"
                     /locus_tag="ACHY03_003456"
     CDS             8237..9529
                     /gene="clcB"
                     /locus_tag="ACHY03_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFS3906647.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQERKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSNAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(9482..10177)
                     /gene="bioD"
                     /locus_tag="ACHY03_003457"
     CDS             complement(9482..10177)
                     /gene="bioD"
                     /locus_tag="ACHY03_003457"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFS3906648.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     SDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(10302..11522)
                     /gene="mlc"
                     /locus_tag="ACHY03_003458"
                     /gene_synonym="dgsA"
     CDS             complement(10302..11522)
                     /gene="mlc"
                     /locus_tag="ACHY03_003458"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFS3906649.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(11657..>12525)
                     /gene="ynfL"
                     /locus_tag="ACHY03_003459"
     CDS             complement(11657..>12525)
                     /gene="ynfL"
                     /locus_tag="ACHY03_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3906650.1"
                     /translation="YFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLART
                     NRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSD
                     TLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLM
                     AMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVG
                     EAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAA
                     RNFRIHLLNALR"
BASE COUNT         3225 a         3152 c         3181 g         2967 t
ORIGIN      
        1 actcaaagaa attgcagaac gcatcagtaa aatatgcaaa aaacacaaaa tcgactttgt
       61 attttctttt tcagtgctta cagaagttgg aaataacgaa tataaagaca gtcgctttgt
      121 ttccttcggt ttatattgcg aaaccaccag cccatatatt catgctgcat ctgaaattgt
      181 cgacagtcat attgatccac aaagaattca ggaactgacc ctggcacttc aacttctcag
      241 agaaaatggc gaatgcaact gctctgactg ccagcatgaa aagaaaaaaa caactcacaa
      301 aacaacaaac caggcaacct tacactgaaa taaaaaatcc ggcagcgcag gctaccggac
      361 tcccccctgc gtcactgtat tcggagaaat cagacaaagg gccgctaatt ctaatccagc
      421 cagaggttta aatacaatga gcgctgataa acaaactttt gcactgcact gcgaagcaaa
      481 aaacgataaa gtcagaaaac gccttggcat caaaggcggt tttttctggg ctgataccag
      541 aaaactttct gtcgcagttt cccgctgcat tgcagccatg gacgatgcag gctacgacga
      601 ggatgatttc aaaaaacccg ttcgcgtaaa tttccccgtc gtgaatgacc ttccaccgga
      661 aggcgtgttt gatactgaat tctgcaaccg ctatgaaaaa ggcggggaag atggcatcac
      721 catgatggca atccccttcg atgacaacat caacggtgaa gatgccacaa cagctggcga
      781 tgactacgat aacctggacg gaactattcc ggatgatgtg gagaaaagcg aatccccgga
      841 cagcgacgat gactgttctg agtgtgaaat tcccgtcgcc actctgagcc ttactcatcg
      901 cttccttcac ctcttcttct tcttcagcaa agatgaagat ggaaaatatc ggcatcacgc
      961 cacacccgaa caacgaaata acgtgatccg tatggagatg gacacagaag acagttacct
     1021 tcagagcctg cttactgctg tgcgttcaca tccagagctg gataagctga cgaattatca
     1081 tctgggaaga ctggcacaat ccgttggaaa ggcattctct cacgcaacac cccgtcatat
     1141 cagcccggcc gaattcgaca agttcatctc cacctggatg aaaacagact atgtggatca
     1201 gggattgctg gcaaaagaat ggcagaaagg aaattatgtt acaggcatca ctcgtacgcc
     1261 ttccggtgct aacgctggcg gcggaaatct taccgatcgt ggcgaaggct ttacccacaa
     1321 tcaggcatca ctggcgcgag acattgccac tggcgttctg gcccgttcaa tggatgtgga
     1381 tatttataac ctgcaccctg cacatgcaaa acgcgttgaa gaaatcgttg cagagaataa
     1441 accgcccttt tctgttttcc gtgacaaatt catcgccatg cccggtgggc tggattattc
     1501 ccgcgccatt gtggtggctt cagtgaaaga agcaccaatt ggcattgagg ctatcccggc
     1561 gcgcgtgact gaatatctca acaaagtgtt gaccgaaacc gatcactcta acccggatcc
     1621 ggaaatcgtg gaaattgcct gcggtcgctc atcagccccg atgccgcagc gcggaacagc
     1681 agaagagata catgacgatg aagaaaagca gcaaacatcg gacgcaatgt ctaatgaaca
     1741 ggcagcgcct gaatcagtgg aagaaattcc tgttaaacat aatgcggaca cgcaatcact
     1801 ggaaaatgtc tcatctgtag aaacgaaata ccaggaactg agagcagaac tctatgaagc
     1861 caggaaaaac attttgccca aaaatcctgt cgatgcagac aaattgctgg ctgcctctcg
     1921 tggggagttc gttgaaggca tcagcgaccc aaccgacccg aagtgggtga aggggatcca
     1981 gactcgcgac actgaggacc agaatcaatc caaagtggaa caaaacagcc cggatacgca
     2041 acaaaacgga ccggaagagc aacagcctgg gccagtaatg caacaggaag tggaaaaggt
     2101 ttgcaccgca tgcggtcaga acagtggcgg gcattgccct gactgtggtc cagtaatggg
     2161 agatgaaact tacgctgaaa cttttggaga aaatgagacc gctgctggag atgaccaggc
     2221 acaaatgaag aatgaataca ttcagaaaaa tgctgttgat acaactcagg aacacgattc
     2281 ggttgttcac atcgatccag atagtgatgc ttctgacaat aatgatgatt cagagcaagc
     2341 agatttcgac tggaaaaggc aactcgtgct tgcagccgtc tatggtttgt gcgcaaaccc
     2401 tgcatgcgtc gcaacagcgc cagcaatccc tgatatcgca atcatgattg ccaacaggct
     2461 tgaaaatttt ggaggtgata aatcatgaat gcctggctta tccccgatcg cattgaagag
     2521 cagtcatggg cacgacacta ccggcaaatt acccgtgaag aaaatgagac tgaactggcg
     2581 gacgacctgg aaaaaggtct gccccagcac ctgtttgaat cgctgtgcat cgataatctg
     2641 caacgtcatg gagccagcaa aaaagccatt gcccgtgcat ttgatgacga tgtcgatttt
     2701 caggaacgca tggcagaaca catccggtac atggttgaaa ccattgcccg ccaccagatt
     2761 tatattgatt cagaggtata aaccagatga gtacagctct cgcaacgctg gcagggaagc
     2821 tggctgaacg tgtcggcatg gattctgtcg acccacagga actgatcacc actcttcgcc
     2881 agactgcatt taaaggtgat gccagcgatg cgcagttcat cgcattgctg atcgttgcca
     2941 accagtacgg ccttaatcca tggacgaaag aaatttacgc cttccctgat aagcagaacg
     3001 gcatcgttcc ggtggtgggc gttgatggct ggtcccgcat tatcaacgaa aaccagcagt
     3061 ttgatggcat ggactttgag cagaacgatg aatcctgtac atgtcggatt taccgcaaag
     3121 accgtaatca cccgatctgc gttaccgagt ggatggatga atgtcgccgc gcaccattca
     3181 aaacccgcga aggcagagaa atcacggggc cgtggcagtc gcatcccaaa cggatgttac
     3241 ggcataaagc gatgattcag tgtgcccgtc tggccttcgg atttgctggt atctatgaca
     3301 aggatgaagc cgaacgcatt gtcgaaaata ccgcatatac tacagaacgt cagccggaac
     3361 gcgacatcac cccggttaac gaagagacca tgtcggaaat taacgccctt cttacttcca
     3421 tggaaaaaac gtgggatgac gacctcttgc cgctctgttc tcagatattt cgccgctaca
     3481 ttcgggcgtc gtcagaactg tcccaggccg aagcagagaa ggttcttgga ttcctgaaac
     3541 agaaagccac ggagcagaag gtggcagcat gacaccagaa attatcctac aacgtaccgg
     3601 gatcgacgtg agaggtgtcg agcagggaga ttatgcatgg caaaaattac ggctcggggt
     3661 catcacagct tcagaagttc acaacgtgat agccaaaccc cgctccggaa agaaatggcc
     3721 tgacatgaaa atgtcctact tccacaccct gctggctgag gtctgcacgg gtgtggcccc
     3781 ggaagttaac gccaaagccc tggcatgggg aaaacagtac gagaacgacg ccagagccct
     3841 gtttgagttt acttccggcg tgaatattac tgaatccccg atcatctatc gcgacgaaag
     3901 tatgcgtacc gcctgctctc cggatggatt atgcagtgac ggcaacggcc ttgagctgaa
     3961 atgtccgttt acctcccggg atttcatgaa attccggctc ggtggtttcg aggccataaa
     4021 atcagcttac atggcccagg tgcaattcag catgtgggtg actcgaaaag atgcctggta
     4081 cttcgccaac tatgacccgc gcatgaagcg tgaaggcctg cattatgtcg tggtcgagcg
     4141 ggatgaaaaa tacatggctg gttttgacga gatggtgccg gagttcatcg aaaaaatgga
     4201 cgaagcactg gctgaaatta gttttgtatt tggggagcaa tggcgatagc cgtagcaacg
     4261 aggtgcaaat gatatgacag ttaaggaatg ctaccggtgt tcgtcctgat ccagcgcggg
     4321 caatctttcg tcgatgccaa caactatccg gtggaaatct gcaaggtaac tcttactcag
     4381 gtgatctacc gaagactcga cggcagaacc cgagccactt caattggtgc atttaatgaa
     4441 gaatttgagc gagtcgacca caacgaacta cacatgatta aagcggaaat tgagaaggaa
     4501 atgcatattg ccagccttcg aaaaatgcga cgtacatcaa tcaactgaca accgccttcg
     4561 ggcggttttt aatggcaaaa atatggattc acacagtatc accctcaaag aggcctgtca
     4621 gtttctcaag atatcaaggc caacagctgt taactggata cgaacgggcc gactacaggc
     4681 aacacgaaaa aattcttccg gtaaaagatc accttatctc acaacccggc aagcctgcat
     4741 tgcagcactt cattcaccgc tgcatactgt ccaggtgagc gcgggtgatg gcataacaga
     4801 ggaaagaaaa tgtcactctt ccgcagaggt gaaatatggt acgcctcgta ctcgctcccg
     4861 ggcgggaagc gaattaagga gtctcttggc acaaaggaca agcggcaagc tcaggagttg
     4921 cacgacaagc gaaaagcaga actctggcga gtagacagac tgggggatat gccagatgtc
     4981 actttcgaag aagcctgcct gagatggctt gaggaaaaag ccgacaagaa atccatcgat
     5041 tccgataaat ccagaatcgc attctggatt gagcatttcg agggaataag gattaaggat
     5101 atatcggagg caatgatcta ctcagttatc agcaaagcgt ataaccgaaa aacaaaggag
     5161 agatggaagt tgcaggtgga ggctgcatta agaaaaggga aagaaccacc agcctatata
     5221 cctaaatcgg tgagcacgca aacaaaagca acacacctgg caatgatcaa ggctattctg
     5281 cgcgccgcag agcgagactg gaaatggctt gaaaaagcac ctgtaatcaa aatacctgcc
     5341 gtaaaaaaca aacgcgtgag atggctggaa aaagaagaag ccaggagact cattgatgca
     5401 tgttctgatc ccctgaaatc tgtagttaaa tttgcactgg caactggcct gaggagatca
     5461 aacattatta atctggagtg gcaacaaatc gatatgcagc gacgtgttgc ttgggtaaac
     5521 cctgaagaca gtaagtcaaa ccgcgctatt ggggtcgcac tgaatgacac tgcctgcaag
     5581 gtgttgcgtg atcaaatagg caaacatcac cgctgggtgt ttgtttatac cactgctgcc
     5641 agaaggcctg acgggacaat gacaccaagc atcagaaaga tgcgcctgga ctataacaca
     5701 tcgtggttaa cagcatgtcg tcgggcagga attgaaaatt tccgttttca tgacctccgc
     5761 cacacttggg ccagttggtt aattcagtca ggtgtaccgc tgtcagtact tcaggaaatg
     5821 ggcggctggg agtctatcga aatggtgcgt aggtacgcac accttgcacc taatcatttg
     5881 acagagcacg cgaggaaaat tgacgacata ttgagtgacg atgtcccaaa tttgtcccac
     5941 cctgaggttt ttgaggatgc aaagaaagca taagcaattg aattagaatg agttaagctc
     6001 acgctttcaa gtggtgagca acaactttaa agatcttggc ccggaagtca gtttccgccg
     6061 tatttatgaa tatgctggcg gcgactggca ggaagataat ggcgtctggc accagaacgt
     6121 gttcgcttat tatctctcca tctcctgtaa ccattgcgat gaccccgcat gtacaaaagt
     6181 ctgccccagc ggcgcaatgc ataagcgtga agatggcttt gtggtggttg atgaagatgt
     6241 ttgtatcggc tgccgctact gccacatggc ttgtccgtac ggcgcgccac agtacaatgc
     6301 tgaaaaaggg cacatgacga agtgcgatgg ttgttattcg cgcgtcgccg aggggaaaca
     6361 acccatatgc gtcgaatcct gcccgctgcg tgcacttgag ttcggtccaa ttgaagagct
     6421 gcgccagaag cacggcactc tggctgccgt cgcgccactg ccgcgcgcgc atttcacaaa
     6481 acccaatatc gttatcaaac ctaacgccaa cagccgcccg accggcgata ccactggtta
     6541 tctggctaat ccggaggaag tgtaaaatgg gaaatggatg gcatgaatgg ccactggtta
     6601 tctttaccgt attgggtcaa tgcgtagtag gcgcgctgat tgttagcgga attggctggt
     6661 tcgccgccaa aaatgacgca gaccgtcagc acattgttcg tggcatgttt ttcctctggc
     6721 tgttgatggg catcgggttt attgcctctg tcatgcacct gggatcgcca ctgcgggcgt
     6781 ttaactcgct gaatcgaatc ggagcttctg gtctaagtaa cgagattgct gccggttcca
     6841 tattttttgc cgttggcgga ctatggtggc tggtggcggt catcggtaaa atgccgcaag
     6901 cgttggggaa actctggtta cttgtcagca tggcgctcgg cgtcattttc gtctggatga
     6961 tgacctgcgt gtatcaaatc gacactgtgc caacctggca taacggttac actacgctgg
     7021 ctttcttcct gacggtattg ttgagtggtc caatacttgc tgccgcaatt ctgcgggcag
     7081 cccgcgttac atttaatacc acaccatttg ccatcattag cgttctggcg ctgatcgctt
     7141 gtgccggggt gattgtgctg caaggtttgt ctctgtcgtc tattcactca tccgtgcaac
     7201 aagccagcgc actggtacca gattacgcct ccttacaggt ctggcgtgtg gtattgttat
     7261 gcgcaggtct tggatgctgg ctttgtccgc tcattcgtcg ccgtgaacct cacgttgccg
     7321 gacttattct ggggctaatc ctcatacttg gcggtgagat gatcgggcgt gtgctctttt
     7381 atggcctgca catgacggta ggaatggcta ttgcaggtta acaaattagt gcggggcaac
     7441 ccgcacattt caggatgtta ggaatgaccc atttttcaca gcaagataat ttttctgttg
     7501 cggcgcgcgt gttgggcgca ctgttttact acgctccaga gagtgctgaa gccgcacctc
     7561 tggttgcggt actcacccgt gacggctggg aaactcagtg gcctctacca gaagcgtcat
     7621 tagcgccgct ggtgacggct tttcagactc agtgtgaaga gactcacgcc caggcctggc
     7681 agcgtttgtt tgtcggcccg tgggcactgc cttcaccgcc gtgggggtcg gtctggctgg
     7741 atcgcgaatc tgtgctgttt ggcgattcaa cattggcact tcgtcagtgg atgcgcgaga
     7801 aaggcattca gtttgaaatg aagcaaaacg aaccggaaga tcattttggt tcactgctgt
     7861 tgatggctgc gtggttggcg gagaatggtc gccagacaga atgcgaagaa ttgctggcat
     7921 ggcacctttt tccgtggtca acacgttttc ttgacgtttt tatcgaaaaa gcagaacacc
     7981 ctttctaccg tgcactgggt gaactggctc gcttaacgct ggcgcaatgg caatcacaac
     8041 tgttaattcc tgtcgcggtt aaatcgctgt ttcgatagga ttaccgtaat attgccggat
     8101 ggtgacgatt acgcgcaatt cggcaataat acgttacagt gtcacgtttt tttatctctt
     8161 aaagcacgca ctgcttttgc ggctggcctc ttttgccgca aaatagtcgc ccgtgtttca
     8221 ttgcccattt ctgctcatgc atcatctaca catctatccg gatctgcgca ctatgttccg
     8281 ccgtctgctt atcgcaacag tcgtcggtat tctcgcggcc tttgccgttg ccgggtttcg
     8341 tcatgcgatg ctgctactgg agtggttgtt cctcaataat gactccggca gtctggtcaa
     8401 tgcagcgaca aatctttccc cctggcgacg gctgctaact ccggcgctcg gcggactggc
     8461 ggcgggtttg ttgttgatgg gctggcagaa atttacccag caacgccctc atgcgccgac
     8521 cgattacatg gaagcgttgc aaaccgatgg tcagtttgat tacgcagcaa gcctggttaa
     8581 atcgcttgcc tctctgctgg tagtaaccag cggcagtgca attggtcgcg aaggtgcgat
     8641 gattctttta gctgcccttg ccgcctcctg ttttgcccaa cgttttacgc cacgccagga
     8701 gaggaaatta tggatcgcct gtggggccgc ggcgggaatg gctgcggcct atcgtgcccc
     8761 gcttgctggc agtttattta tagccgaagt gctgtttggc actatgatgt tggcctctct
     8821 cggcccggtg attatttccg ccgtcgtggc attgctggtt agcaatctga ttaatcatag
     8881 caacgcgtta ctctacagcg tacaactctc agtgacggtt caggctcgtg actatgcgct
     8941 gattatcagt acaggtgtgc tggcaggtct gtgcggacca ctgttgttaa cgttaatgaa
     9001 cgcctgtcat cgtggatttg tgagtctcaa acttgcgccg ccctggcaac tggcactagg
     9061 cgggttgatc gtgggtctgc tttccctgtt cacacctgca gtgtggggca acggctatag
     9121 caccgtacaa tcctttttaa ccgccccgcc actgttaatg atcattgccg ggatcttcct
     9181 ttgtaaactg tgtgccgtgc tggcgagtag cggttccggc gcacccggtg gtgtctttac
     9241 accgacgcta tttatcggtc ttgccattgg catgttgtat ggtcgtagcc tgggattatg
     9301 gttccctgat ggcgaagaaa ttacgctttt actcggattg accgggatgg cgacactgtt
     9361 ggcggcaacc acgcacgcgc cgattatgtc gacgttgatg atatgtgaaa tgaccgggga
     9421 gtatcagcta ctccccggtt tattgattgc ctgcgtaatt gcgtcggtaa tctcgcggac
     9481 gttacaccgt gactctatct accgccagca cactgcgcag catagctaag cggatgtatt
     9541 gccccagttc acgctgttcg gcgcgcggca gataaggcag ttcaccaatg agcggtgccg
     9601 gaagtttttt accgagcaca tcaatgattt ccgcataatg cgcaagtcct gggttgattc
     9661 ggttagccac ccagccaatg agcggcagcc cgtcgctggc aatcgcctga gctgttagca
     9721 gtgcatggtt aatgcaacct tcctgaatac cgacaaccat caacaccggc agttgttcct
     9781 gcaccaccca ttcagagagt ggacgcaaat cattcatcag actgcgccag ccgccagtcc
     9841 cttctaccac gacatgatcg actttatcgg tcaggtttgc caggccgttt gaaatgaggg
     9901 tgtaattgat tgggcaactg tgcgccacgc tactttcttc ttcgcttaat gcgataggat
     9961 taactgcttc ataaggcagt tcgatggttg aaacactctg caacaccagg gcatctttat
    10021 tacgcagccc ttcgggtgtc tctttgctcc ccttcgctac gggtttatat cccgcaaccg
    10081 tttttccctg ggaggctaac gcttgtagca atgcgcggga aaccaccgtt ttccctacag
    10141 aagtgtctgt accggtaata aagaaacgct tcagcatcac taactccacc gttatgcttc
    10201 acaaatataa accaggaaaa taattaacct tgaaagtcta agttatgctt tcctggccca
    10261 aattgagata gcgcaaattt tggtagaaca gttaaaaaat gttaaccctg caacagacga
    10321 atcaacaaag aaccgttata catcgcgtct tttaccagcg cagcgcctgc catcgtaccc
    10381 tggttagaaa actgagtact ctcaacgcta atgtgctgac tatacgcagg aagggcctgc
    10441 tgacggatgc tgtctgagat gaccgggaag aggatatctg ccgctttact taacggtgag
    10501 ccaatcagta ttttttgtgg gttaaataaa ttcaccatga tggcaagaat gcgccctaca
    10561 tgcgcgccca ccccggtaat gatgtctttt gccagcagat cgccgcgcaa tgccgcctga
    10621 cacaatgagt ccacggttaa cggttgccca tgtaacatcg agctcatgga ttgattgaga
    10681 cgcagctgtg ccagctcaag aatactgtcc acactggcga tggtttcgag gcagccgtgg
    10741 ttcccgcaat aacagcgttt accatatggg tctacctgtg tgtgacctat ttccacgagg
    10801 ctactgctgc ctgcgtgtag cagatgacca tcggtaatga cgcccgcccc cacgttgtga
    10861 tcgataacca cctgaatcac atcgcgcgcc ccgcgtgagg caccaaacaa ggcctctgcc
    10921 atcgtccatg cgctgatatc atgctgtata taaaccggaa cgccggtatg ctgctccaga
    10981 gcctcgccga gcggcatctc ttttacatcc tcgtagaacg gcatgcgatg tacaatgcca
    11041 ttttccgtat caataattcc cggcagggtt atggcaatcg aagttagacg ctcaagtttt
    11101 ttctggtggc ggataaaaaa ctgatcgata tgggaaatga tacgatccag caatggcgag
    11161 tcatctttta acgccagttc ctgcgactct tccaccacca gtttgctgct cagatcgcgc
    11221 agagcaagga aaatctcccc gcgactaatg cgcagagaaa gatagtgcca ggcttcagtt
    11281 tcaaccacca gccccaccgc cggacggcca cggttccccg cttctttgat ttccagctct
    11341 tgcaccaggt gtgcttcgag catctcacgg acaattttag tgatactggc aggagccagc
    11401 tgcgccagac gggaaagatc gatacgcgag actggaccaa gctgatcaat caggcgataa
    11461 accgcgcccg cgttggtctg ctttatttga tcaatgtgcc caggctggtt ttcagcaacc
    11521 accgcatact ccctatattt tcgcgctccg aaataatctg taggctatgg tgaagcactt
    11581 caatacgtgt cgtcaaattt ttacttaggc atgtgattaa cagcacattt ttcgggcttt
    11641 ttcgctgaaa tttccctcac ctgagagcat tcagcagatg aatacgaaag ttacgcgcag
    11701 ctggactttg ttcatgatgt ttcggccaga ccaaccacat ttcagaaacc gcatcctctt
    11761 cagcaatcgg cacccagcgc atttcgttga gctgaacacg tttaaatgac gcaggcaaaa
    11821 ttgaaacacc cagaccggcg gaaaccagac cgatgatggt cattgcctcg cccacctcct
    11881 gagtgatgac gggcgtcaaa tggtaacgtc gcatcagccc gagaatatcg tcatacagcc
    11941 ctgtcccgac gtgcggatca aaaaagacaa agggttcttt cgccagttca gccagcgtta
    12001 cattcgggtt atttgccagg ggatgatcgt gcgggatcat cgccataagc ggttcatgga
    12061 cgatgactgc gtgttcaagc gtctccggta acgctgtgtt acgcagcaat cccatatcca
    12121 gcgttccttc aatgagcgga gcgatttgct cgcgagtgtt catttcgcgg gtttgtaaat
    12181 ggacatcggg ataatctcgg cgaaacagcg ataacgtatc ggacaccgcc cgaataaaag
    12241 gagccgacga agtaaaacca atgcgcaact cccccgcttc accctgatgc agtctttcag
    12301 cgcgagcggc agcgtcatcc accatagaca ggatttgccg actatctgca agaaactgtt
    12361 ttcctgctgc cgtcagcaat acactgcgat tggttcgtgc cagcagtcgg gcaccaattt
    12421 gttgctccag cgcctgaatc tgctgactta gcggcggttg cgaaatattc aggcgggcag
    12481 cggcgcgccc gaaatgcagc tcttccgcaa cagcaacaaa gtaac
//
LOCUS       DAZUCE010000147        12510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZUCE010000147 DAZUCE010000000
VERSION     DAZUCE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.147"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(601..685)
                     /locus_tag="ACHY03_003460"
     tRNA            complement(601..685)
                     /locus_tag="ACHY03_003460"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(649..651),aa:Leu,seq:caa)
     gene            881..1900
                     /gene="ahr"
                     /locus_tag="ACHY03_003461"
     CDS             881..1900
                     /gene="ahr"
                     /locus_tag="ACHY03_003461"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFS3906651.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            2030..3532
                     /gene="yjgR"
                     /locus_tag="ACHY03_003462"
     CDS             2030..3532
                     /gene="yjgR"
                     /locus_tag="ACHY03_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFS3906652.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRPNPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASTEQQNNPAVKRKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(3693..4775)
                     /gene="lptG"
                     /locus_tag="ACHY03_003463"
     CDS             complement(3693..4775)
                     /gene="lptG"
                     /locus_tag="ACHY03_003463"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFS3906653.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(4775..5875)
                     /gene="lptF"
                     /locus_tag="ACHY03_003464"
     CDS             complement(4775..5875)
                     /gene="lptF"
                     /locus_tag="ACHY03_003464"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFS3906654.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(5938..5988)
                     /gene="ytgA"
                     /locus_tag="ACHY03_003465"
     CDS             complement(5938..5988)
                     /gene="ytgA"
                     /locus_tag="ACHY03_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFS3906655.1"
                     /translation="MSIIILATISTTQENK"
     gene            6142..7653
                     /gene="pepA"
                     /locus_tag="ACHY03_003466"
     CDS             6142..7653
                     /gene="pepA"
                     /locus_tag="ACHY03_003466"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFS3906656.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8007..8450
                     /gene="holC"
                     /locus_tag="ACHY03_003467"
     CDS             8007..8450
                     /gene="holC"
                     /locus_tag="ACHY03_003467"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFS3906657.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8450..11305
                     /gene="valS"
                     /locus_tag="ACHY03_003468"
     CDS             8450..11305
                     /gene="valS"
                     /locus_tag="ACHY03_003468"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFS3906658.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11361..>12510)
                     /gene="yjgN"
                     /locus_tag="ACHY03_003469"
     CDS             complement(11361..>12510)
                     /gene="yjgN"
                     /locus_tag="ACHY03_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFS3906659.1"
                     /translation="FHGTGERYFLICVVNVLLTIITLGIYLPWALMKCRRYLYANMEV
                     NGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCVLTLSLLVLLIFMAAKG
                     LRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSV
                     IISVVLMAIVGIVSIGSFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYCIKYAILAF
                     LALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAV
                     STSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLK
                     IWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIMPSLPFL"
BASE COUNT         3217 a         3186 c         3317 g         2790 t
ORIGIN      
        1 taaccaatga aatctacaac aagcaactga tggataatcc gatattggtc gcatttaatg
       61 gcatggtagc aaagttttca tcacatacct cctttgcacc agaataaacg ccagagatct
      121 gaaattgcga gtacactaca gaaattgtac tgccggtccc atcagcataa aaccatagtt
      181 acacaggtaa tcatcattat tcgccaagat cataaatatt attcggtaga atagcgttct
      241 caggtggtca gtaaaattgt cttcaacaag atgtctgcgc atctcgtctg ctagccctac
      301 ataactgctc tcgaccgaaa ctatttattg aatgttcacc aaggtgggcg tggagacaaa
      361 atgacgtctt ttgatattag ctacctgagc cacatggtca aaacgttcaa gtagcatagc
      421 gaaaccaccc tgcccacgta atgtggacga tgcagcttcc agccagagca aacttgggac
      481 catcgactac aaaatgaaga atactgaact gcttttctgg caggcctata tactgcacgg
      541 atatgggtga tttcagacac aaaaaaagcc gctcttgagc gactcgattt gcatacggtg
      601 tggtgccgaa ggccggactc gaaccggcac gtatttctac ggttgatttt gaatcaactg
      661 cgtctaccga tttcgccact tcggcactga agaggtatgc ggaaaacgtt gtggattata
      721 cctgtcgcac gccaccatgc aagcgccagc ttgcacccac tttgctaagt gctgaaattt
      781 tcagcattac actcagcgat gtgtaacctt tgtcacactc caggcacccc gccctgccat
      841 gctctaaact tcccagacaa caccagagaa ggaccaaaaa atgtcgatga taaaaagcta
      901 tgccgcaaaa gaagcaggcg gcgaattgga agtttatgag tacgatcccg gtgaactgaa
      961 gccacaagat gttgaagtgc aggtggatta ctgcgggatc tgccattccg atctgtcgat
     1021 gatcgataac gaatggggat tttcacaata tccgctggtt gccgggcatg aggtgattgg
     1081 tcgcgtggtg gcgctcggga gcgccgcgca ggataaaggt ttgcaggtcg gtcagcgtgt
     1141 cgggattggc tggacggcgc gtagctgcgg gcattgcgat gcctgtatta gcggtaatca
     1201 gatcaactgt gagcaaggtg cggtgccgac gattatgaat cgcggcggct ttgccgagaa
     1261 gttgcgtgcg gactggcaat gggtgatccc actgccagaa aatatcgata tcgagtccgc
     1321 cgggccgctg ctgtgtggtg gtatcacggt cttcaaacca ctgttgatgc accatatcac
     1381 tgctaccagc cgcgttgggg taattggtat tggcgggctg gggcatatcg ctataaaact
     1441 tctgcacgca atgggatgcg aggtgacagc ctttagttct aatccggcga aagagcagga
     1501 agtactggcg atgggtgccg ataaagtggt gaatagccgc gatccgcagg cactgaaagc
     1561 actggcgggg cagtttgatc tcattatcaa caccgtcaac gtcagcctcg actggcagcc
     1621 ttattttgag gcgctgacct atggcggtaa tttccatacg gtcggtgcgg ttctcacgcc
     1681 gctgtctgtt ccggccttta cgttaattgc gggcgatcgc agcgtctctg gttctgctac
     1741 cggcacgcct tatgagctgc gtaagctgat gcgttttgcc gcccgcagca aggttgcgcc
     1801 gaccaccgaa ctgttcccga tgtcgaaaat taacgatgcc atcaagcatg tgcgcgacgg
     1861 taaggcgcgt taccgcgtgg ttctgaaagc tgacttctga tctctttgcc gggtgcctgt
     1921 cgcccggcac ttaaatctct ttggaaggcg tctcgcaatc tgctgaattt agcggtgaag
     1981 agtgcgcagc cgttgcctat actccaggca ggaaactgga ggaaatctca tgagcgaacc
     2041 cctgttaatt gcccgcacgc cggacacaga actattttta ctgccgggaa tggctaaccg
     2101 tcacgggctg attactggcg caacggggac gggtaaaact gtcacgttgc aaaaactggc
     2161 agagtcattg tcggaaatcg gcgtgccggt gtttatggct gatgtgaaag gcgatctgac
     2221 cggcgtcgcg caggcaggaa cggcgtcgga aaaactgctc gcaaggctta aaaatatcgg
     2281 cgtaaatgac tggcaaccgc atgctaatcc ggtggtggtg tgggatatct ttggcgagaa
     2341 aggccatccg gtgcgggcga cggtttcaga cctggggccg ctgttgctgg cgcggctgtt
     2401 gaatctcaac gatgtgcaat ctggcgtgct gaatatcatc ttccgcattg ctgacgatca
     2461 ggggctgtta ctgctcgact ttaaagattt gcgggcgatt acccagtaca tcggcgataa
     2521 cgccaaatct ttccagaatc agtacggaaa tatcagtagc gcatcggttg gtgccatcca
     2581 gcgcgggctg ttgtcgctgg aacagcaagg cgcagcacac ttctttggtg agccgatgct
     2641 ggatatcaaa gactggatgc gcaccgatgc caacggtaaa ggcgttatca atatcctcag
     2701 cgccgagaaa ctttatcaga tgccgaaact gtacgccgcc agtctgctgt ggatgctttc
     2761 agagttgtat gaacaattgc cggaagcggg cgatctggag aagccaaaac tggtgttttt
     2821 cttcgacgaa gcacatctgc tgtttaacga tgcaccgcag gtactgctgg ataagattga
     2881 gcaggtgata aggcttattc gctcaaaagg cgtgggcgtc tggttcgttt cgcaaaaccc
     2941 gtctgatatt ccggataatg tgctcggcca gctcggtaat cgcgttcagc acgctttgcg
     3001 ggcttttacg ccaaaagatc agaaagcagt gaaggcagcg gcgcaaacca tgcgccccaa
     3061 ccctgcattt gatacggaaa aggcaatcca ggaactgggg accggcgagg cgttaatctc
     3121 gtttctcgat gcaaaaggaa gtccttctgt ggtggaacgg gcgatggtga ttgcgccttg
     3181 ttcgcggatg ggaccggtga cggaagatga gcgtaatggc ttgattaatc actctccggt
     3241 gtatggcaag tacgaggatg acgtggaccg ggaatccgcc tatgagatgt tgcaaaaagg
     3301 cgtacaggca agcaccgagc agcaaaataa tcctgccgtg aaaaggaaag aggtggcggt
     3361 ggatgacggc attcttggtg gattgaagga tattttgttt ggcactaccg gaccacgcgg
     3421 cgggaagaaa gatggtgtgg tgcaaacaat ggccaaaagc gccgctcgcc aggtgacgaa
     3481 tcagattgta cgcgggatgt tggggagttt gctggggggg agaaaaaggt aagccaggtg
     3541 cgatttgtct ttgcctgatg cgacgctggc gcgtcttatc atgcctacac cactgcaata
     3601 tattggattt tcataatttt tcaggaatga acgaagcaca ctttgctaac aataataaag
     3661 ggagctttcg ctccctttat tcgttcattc ggttacgatt ttctcattaa cagccacagg
     3721 ctgattaaga agaagctggc gcttggcaac agtgcgccga tgatcggcgg gatgccataa
     3781 accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc gaagctgata
     3841 ccggtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat gaacgacagc
     3901 gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca catgttgagc
     3961 tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt gtgcaaacca
     4021 ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtccgg cgtgaggttg
     4081 gttttccagg tgccgctcac cgtctgcgaa ccggtgatct gtttcggatt ggtcagatca
     4141 gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt cgcggtagcg
     4201 gcatagcgta cggattgcag acgacgattc tcgttaaagg catagatact gatgccacct
     4261 aactcttcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc tttcgcccat
     4321 aagccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg gtagttacgc
     4381 gccatctgct cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag caagaccagc
     4441 ggaatggcgg ttttcatcac cgacagcgcc acctgcatac gggtaaaacc agaagcctgc
     4501 atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc cccaagcaga
     4561 gccgccatcg ggaagaagat ttgcacatct ttcggcacgc tcagcaaggt atacattcct
     4621 gcacctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac aaacttgata
     4681 atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa aatagtttta
     4741 ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta cgcgaaaaac
     4801 tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc gccaaagcca
     4861 gataaatcag gttaacggtc cacatccaca gcgtcgggtc cagcttacct ttaccgccgt
     4921 tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg gctggcagca
     4981 tcgacagtac gcgtccctga cgtgggttaa ccacgctcag cggtacgacc ataagtgcca
     5041 tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga gcacgatcgg
     5101 tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg tcgagcgcca
     5161 ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg aaatcacgta
     5221 acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg gagccgtcgc
     5281 gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca ttaccttttg
     5341 gtcgaatttg cgcgaggaac acatctttga aatcgctgcc gtcaacgctt tcgatgaaca
     5401 gcaccgaact gccattagtc gcttgctgga attgcccttg cgccagcgcc gccatgccag
     5461 ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac ggtcccgccc
     5521 acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt gccgctttca
     5581 ccagaaccgc tttgctcagg ccgcaggcat gcattaccgt aatttcactt tcggtataca
     5641 gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata agctgcgcca
     5701 tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata tcgccgtcaa
     5761 ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc aagatgaaga
     5821 gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt atcactttaa
     5881 atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc ttaaacgtca
     5941 tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg gattcacctc
     6001 tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac gaagtcatcg
     6061 caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg ccgttgtctt
     6121 taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc ggagaaacag
     6181 cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc gattgcagaa
     6241 cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgtcgggg cgaactggaa
     6301 ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc cgagcgaatt
     6361 ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa gcaggttatt
     6421 cagaaaacca ttaatacgct gaatgatact ggctcaatgg aagcggtctg ctttctgact
     6481 gaactgcacg ttaaaggccg taataactac tggaaagtgc gtcaggctgt cgagacggca
     6541 aaagagacgc tctacagttt cgatcagctg aaaacgaaca agagcgaacc gcgtcgcccg
     6601 ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg tgagcgcgcg
     6661 atccagcacg gtctggcgat tgccgccggg attaaagcag cgaaagatct cggcaatatg
     6721 ccgccgaata tctgtaacgc cgcttacctc gcttcacaag cgcgccagct ggctgacagc
     6781 tacagcaaga atgttatcac ccgcgttatc ggcgaacagc agatgaaaga gctggggatg
     6841 cattcctatc tggcggtcgg tcagggttcg caaaacgaat cgctgatgtc ggtgattgag
     6901 tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa aggtttaacc
     6961 ttcgactccg gcggtatctc gatcaagcct tcagaaggca tggatgagat gaagtacgat
     7021 atgtgcggtg cggcagcggt ttacggcgtg atgcggatgg tcgcggagct acaactgccg
     7081 attaacgtta tcggcgtgtt ggcaggctgc gaaaacatgc ctggcgggcg tgcctatcgt
     7141 ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa taccgacgct
     7201 gaaggccgcc tggtactgtg cgacgtgtta acttacgttg agcgttttga gccggaagcg
     7261 gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca tcacattacc
     7321 ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc tgaacaatcc
     7381 ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacaact ggagtccaat
     7441 tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc aggttgcttc
     7501 ctgtcgcgct ttacccgtaa gtacaactgg gcgcacctgg atatcgccgg taccgcctgg
     7561 cgttctggta aagcaaaagg cgccaccggt cgtccggtag cgttgctggc acagttcctg
     7621 ttaaaccgcg ctgggtttaa cggcgaagag taattgcgtc aggcaaggct gttattgccg
     7681 gatgcggcgt gaacgcctta tccgacctac acagcactga actcgtaggc ctgataagac
     7741 gcgcaagcgt cgcatcaggc aatgctgtta ttgtcggatg cggcatgaac gccttatccg
     7801 acctacacag caccgaactc gtaggcctga taagacgcgg cagcgtcgca tcaggcaatg
     7861 ctgttattgt cggatgcggc gtgagcgcct tatccgacct acacacagca ctgaactcgt
     7921 aggcctgata agacacaaca gcgtcgcatc aggcgctgcg gtgtatacct gatgcgtatt
     7981 taaatccacc acaagaagcc ccatttatga aaaacgcgac gttctacctt ctggacaatg
     8041 acaccaccgt cgatggctta agcgccgttg agcaactggt gtgtgaaatt gccgcagaac
     8101 gttggcgcag cggtaagcgc gtgctcatcg cctgtgaaga tgaaaagcag gcttaccggc
     8161 tggatgaagc cctgtgggcg cgtccggcag aaagctttgt tccgcataat ttagcgggag
     8221 aaggaccgcg cggcggtgcg ccggtggaga tcgcctggcc gcaaaagcgc agcagcagcc
     8281 cgcgcgatat attgattagt ctgcgaacaa gctttgcaga ttttgccacc gctttcacag
     8341 aagtggtaga cttcgttcct tatgaagatt ctctgaaaca actggcgcgc gaacgctata
     8401 aagcctaccg cgtggctggt ttcaacctga atacggcaac ctggaaataa tggaaaagac
     8461 atataaccca caagatatcg aacagccgct ttacgagcac tgggaaaagc agggctactt
     8521 taagcctaat ggcgatgaaa gccaggaaag tttctgcatc atgatcccgc cgccgaacgt
     8581 caccggcagt ttgcatatgg gtcacgcctt ccagcaaacc atcatggata ccatgatccg
     8641 ctatcagcgc atgcagggca aaaacaccct gtggcaggtc ggtactgacc acgccgggat
     8701 cgctacccag atggtcgttg agcgcaagat tgccgcagaa gaaggtaaaa cccgtcacga
     8761 ctacggccgc gaagctttca tcgacaaaat ctgggaatgg aaagcggaat ccggcggcac
     8821 gattacccgt cagatgcgcc gtctcggcaa ctccgtcgac tgggagcgtg aacgcttcac
     8881 gatggacgaa ggcctgtcca atgcggtgaa agaagttttc gttcgtctat ataaagaaga
     8941 cctgatttac cgtggcaaac gcctggtaaa ctgggacccg aaactgcgca ccgctatctc
     9001 tgacctggaa gtggaaaacc gcgaatcgaa aggttcgatg tggcacatcc gctatccgct
     9061 ggctgacggt gcgaaaaccg cagacggtaa agattatctg gtggttgcga ctacccgtcc
     9121 ggaaaccctg ctgggcgata ctggcgtagc cgttaacccg gaagatccgc gttacaaaga
     9181 tctgattggc aaatatgtca ttctgccgct ggttaaccgt cgtattccga tcgttggcga
     9241 cgaacacgcc gacatggaaa aaggcaccgg ctgcgtgaaa atcactccgg cgcacgactt
     9301 taacgactat gaagtgggta aacgtcacgc gctgccgatg atcaacatcc tgacctttga
     9361 cggcgatatc cgtgaaagcg cccaggtgtt cgataccaaa ggtaacgaat ctgacgttta
     9421 ttccagcgag atcccggcag aattccagaa actggagcgt tttgccgcac gtaaagcagt
     9481 cgttgctgcg gttgacgcac tcggcctgct ggaagaaatt aaaccgcacg acctgaccgt
     9541 tccttacggc gaccgtggcg gcgtggttat cgaaccaatg ctgaccgacc agtggtacgt
     9601 acgtgccgat gtactggcga agccggcggt tgaagcggtt gagaacggcg acattcagtt
     9661 cgtaccgaag cagtacgaaa acatgtattt ctcctggatg cgcgatattc aggactggtg
     9721 tatctctcgt cagctgtggt ggggtcaccg tatcccggca tggtatgacg aagcgggtaa
     9781 tgtttatgtt ggccgcaacg aagaagaagt gcgcaaggaa aataacctcg gtgccgacgt
     9841 tgccctgcgt caggacgaag acgttctcga cacctggttc tcttctgcgc tgtggacctt
     9901 ctctaccctt ggctggccgg aaaacaccga cgccctgcgt cagttccacc caaccagcgt
     9961 gatggtatcc ggcttcgaca tcatcttctt ctggattgcc cgcatgatca tgatgaccat
    10021 gcacttcatc aaagatgaaa atggcaaacc gcaggtgccg ttccacaccg tttacatgac
    10081 cggcctgatt cgtgatgacg aaggccagaa gatgtctaaa tccaagggta acgttattga
    10141 tccgctggat atggttgacg gtatttcgct gccagaactg ctggaaaaac gtaccggcaa
    10201 tatgatgcag ccgcagctgg cggacaaaat ccgtaagcgc accgagaagc agttcccgaa
    10261 cggtattgag ccgcacggta ctgacgcgct gcgcttcacc ctggcggcgc tggcgtctac
    10321 cggtcgtgac atcaactggg atatgaagcg tctggaaggt taccgtaact tctgtaacaa
    10381 gctgtggaac gccagccgct ttgtgctgat gaacacggaa ggtcaggatt gcggctttaa
    10441 cggcggtgaa atgacgctgt cgctggcgga tcgctggatt ctggctgagt tcaaccagac
    10501 catcaaagcg taccgcgaag cgctggacag cttccgcttc gatatcgctg caggcattct
    10561 gtatgagttc acctggaacc agttctgtga ctggtatctg gagctgacta agccggtaat
    10621 gaacggtgga accgaagccg aactgcgtgg tactcgccat acgctggtga ctgtactgga
    10681 aggtctgctg cgcctcgcgc atccgatcat tccgttcatc accgaaacca tctggcagcg
    10741 tgtgaaagta ctttgcggta tcactgccga caccatcatg ctgcagccgt tcccgcagta
    10801 tgatgcatct caggttgatg aagcggcgct ggccgacacc gagtggctga aacaggcgat
    10861 cgttgcggta cgtaacatcc gtgcagaaat gaatatcgcg ccgggcaaac cgctggagct
    10921 gctgctgcgt ggttgcagcg cggatgcaga acgtcgcgta aatgaaaacc gtggcttcct
    10981 gcaaaccctg gcgcgtctgg aaagcatcac cgtgctgcct gccgatgaca aaggtccggt
    11041 ttccgttacg aagatcatcg acggcgcaga actgctgatc ccgatggctg gcctcatcaa
    11101 caaagaagat gagctggcgc gtctggcgaa agaagtggcg aagatcgaag gcgaaatcag
    11161 ccgtatcgag aacaagctgg cgaatgaagg ctttgtcgcc cgcgcaccgg aagcggtcat
    11221 cgcgaaagag cgtgagaagc tggaaggcta tgcggaagcg aaagcgaaac tgattgaaca
    11281 gcaggctgtt atcgccgcgc tgtaatcaaa acacagtgat gaaaacgaag gccggagcat
    11341 gctccggcct tttttatctc ttacagaaat ggtaaagaag gcataattcc ccgtgaaata
    11401 ctggctaaga atcctttatc tggttgttct tctttattga ttaaaggaag gtcatccaaa
    11461 tcgcccagca aatacgtatt ttttgcctgc cagtcaatca tccatatttt cagcagtgga
    11521 taagccagac cgcccgtaat cccggatatc accaccaacg cacacatgcg ataaagcata
    11581 ccgtggtatg ttaaagttga gcgaaaacga atacgcccat cattcagtga caggttgctc
    11641 ataaaatgat ttcgcaacga caccgttaaa taactggtgc taacagcaat cccaaaataa
    11701 tagattaact gcgcgattat cattttacgt tgcatttcca taaattgctg taaattctca
    11761 atatcatcat ttgcatacac agaactatca tacgcattta atatttgatc gaagataata
    11821 taaccagcaa cagcgagaaa aggtaataaa gctaaaaatg cgagaatggc atattttata
    11881 caatacgtag tatctaattt caccttgaaa cgatgtatac cgaaactggt attgctccag
    11941 agaaaactca ttaccagact atataaagta ccattaaaac taccaatgga aacaatacca
    12001 actattgcca tcagaaccac agatataata acactacttg aactattggc atgtagcatc
    12061 tttgtcgaga taaagaaaac agtccccatc ccaatggcca ttaaaatcgg caagaaaaag
    12121 gttacccacc agaacccttt caaagagcaa ttaaaactaa atcttacgcc gttgagactg
    12181 gtcatcaagg cctgataacg cagtcctttt gctgccataa atataagcaa aaccaacagt
    12241 gacaaagtca aaacacagcc aacaagcggc atatctgctg acactgtcat taagattgcg
    12301 aaatagaaaa aaacaaaaac aagacaacta aaaaaaacat tcccaccagt aattccataa
    12361 gaaaatcgtt gtccgttaac ttccatatta gcatagagat aacgcctaca tttcattaat
    12421 gcccatggta aatagatacc tagcgttata atcgttaaca acacattcac cacacaaata
    12481 agaaaatatc tctcacctgt accatgaaaa
//
LOCUS       DAZUCE010000148        12454 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZUCE010000148 DAZUCE010000000
VERSION     DAZUCE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.148"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            58..1110
                     /locus_tag="ACHY03_003470"
     CDS             58..1110
                     /locus_tag="ACHY03_003470"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFS3906660.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1186..2619
                     /locus_tag="ACHY03_003471"
     CDS             1186..2619
                     /locus_tag="ACHY03_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFS3906661.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2802..5063
                     /locus_tag="ACHY03_003472"
     CDS             2802..5063
                     /locus_tag="ACHY03_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFS3906662.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGQIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEAHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5204..6487)
                     /locus_tag="ACHY03_003473"
     CDS             complement(5204..6487)
                     /locus_tag="ACHY03_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFS3906663.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6639..7115)
                     /locus_tag="ACHY03_003474"
     CDS             complement(6639..7115)
                     /locus_tag="ACHY03_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFS3906664.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(7174..8463)
                     /gene="bioA"
                     /locus_tag="ACHY03_003475"
     CDS             complement(7174..8463)
                     /gene="bioA"
                     /locus_tag="ACHY03_003475"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFS3906665.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRIYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEAQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            8550..9590
                     /gene="bioB"
                     /locus_tag="ACHY03_003476"
     CDS             8550..9590
                     /gene="bioB"
                     /locus_tag="ACHY03_003476"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFS3906666.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            9587..10741
                     /gene="bioF"
                     /locus_tag="ACHY03_003477"
     CDS             9587..10741
                     /gene="bioF"
                     /locus_tag="ACHY03_003477"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFS3906667.1"
                     /translation="MSWQDKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQSAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFIHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTSAHEMQDIDRLLEVLHGNG"
     gene            10728..11483
                     /gene="bioC"
                     /locus_tag="ACHY03_003478"
     CDS             10728..11483
                     /gene="bioC"
                     /locus_tag="ACHY03_003478"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFS3906668.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHVQVTALDLSPPMLIQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVHPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHRIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            11476..12153
                     /gene="bioD"
                     /locus_tag="ACHY03_003479"
     CDS             11476..12153
                     /gene="bioD"
                     /locus_tag="ACHY03_003479"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFS3906669.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESSVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YIDLALL"
BASE COUNT         2971 a         3123 c         3367 g         2993 t
ORIGIN      
        1 ttgcccttaa tgtaaccata tcgcaataag ttatgttttt aaattgaggg cattattatg
       61 aaaaaaatac cctgcgtgat gatgcgaggt ggaacctcga ggggcgcgtt cctgttagcg
      121 gaacatttac ccgaagatca aacgcagcgc gataaaatat tgatggcaat tatgggttcc
      181 ggtaatgatc tggaaattga cggtattggc ggcggtaatc cactgaccag taaagtcgcc
      241 attattagcc gttccagcga tccgcgtgct gatgttgatt atctgtttgc tcaagttatc
      301 gtccatgagc aacgtgtcga taccacgcct aactgcggca atatgctgtc tggcgttggc
      361 gcattcgcca ttgaaaatgg tttgattgca gcgacttcgc cagttacccg tgtacgtatc
      421 cgcaacgtca atacgggtac gttcatcgaa gctgatgtgc aaacgccaaa tggtgttgtc
      481 gagtacgagg gtagcgccag aattgacggc gtaccgggta ctgccgcacc ggttgcgctc
      541 actttcctga atgccgctgg aacgaaaacc ggaaaagttt tcccgactga taatcagatt
      601 gattattttg acgatgtccc ggtgacctgt atcgatatgg cgatgccagt cgtcattatt
      661 ccggctgaat atctgggaaa aacaggttat gaattaccgg cggaactgga tgccgacaaa
      721 gcattattag cccgcattga atctatccgt ctacaagcgg gtaaagcaat gggcttaggt
      781 gatgtcagta atatggttat ccctaaacct gtgcttattt ctccagcgca gaaaggcggg
      841 gcaattaatg tgcgttattt tatgccgcat tcttgccatc gcgcgctggc gataaccggt
      901 gctattgcta tttccagtag ttgtgcattg gaaggcaccg tcacccgaca aatcgtccct
      961 tctgtaggat atggcaatat caatattgaa caccccagtg gtgcgctcga cgttcattta
     1021 agtaatgaag gtcaggatgc cacgacgtta cgcgcatctg ttattcggac aaccagaaaa
     1081 atattttccg gtgaagttta tcttccctga aaaaattcgt tgtcaggata aggacaatca
     1141 ataaaggact tctgtatgag tcatacagaa agaacaggat tttaaatgaa taagaaatcg
     1201 ttatggaagc taattctgat attagcgatc ccatgtatta ttggctttat gccagctccg
     1261 gcaggattaa gcgaactggc gtgggtgctt tttggtattt acctggcggc cattgtgggt
     1321 ctggttatca agcctttccc ggaacctgtc gtactgttaa ttgccgttgc tgcctcaatg
     1381 gtggtggtcg gtaacttatc cgacggtgcg tttaagacca ccgccgtatt aagcggttac
     1441 tcttcaggta ctacctggct ggtgttctcg gcgtttacct taagcgccgc atttgtgacc
     1501 accggtttag gtaaacgtat tgcctatctg ctgattggta aaatcggtaa caccacgctg
     1561 ggtctgggtt acgttacggt attcctcgat ctggtattgg ctccggcaac accgtctaac
     1621 accgcgcgtg cgggcggcat tgtgttaccg atcatcaaca gcgtggcggt ggctttgggg
     1681 tccgaaccgg aaaaaagtcc gcgtcgtgtc ggacattacc tgatgatgtc catttacatg
     1741 gtcaccaaaa ccaccagcta tatgttcttt accgcaatgg cggggaacat tctggcgctg
     1801 aaaatgatca acgacattct gcacctgcaa attagctggg ggggatgggc gctggccgcc
     1861 ggattgccgg gcatcattat gctgctggtt actccgctgg tgatttacac catgtatccg
     1921 ccagaaatta agaaggtgga taacaaaacc atcgctaaag cgggccttgc cgaactggga
     1981 ccgatgaaaa tccgcgaaaa aatgctgctc ggtgtctttg tgctggcgct gctgggctgg
     2041 attttcagta agtctctggg ggttgatgaa tccaccgtgg caatcgttgt tatggcgact
     2101 atgctgctgc tgggtatcgt tacctgggaa gacgtggtta aaaataaagg cggctggaat
     2161 accttaatct ggtacggcgg tattatcggc ttaagctcct tattatcgaa agttaaattt
     2221 ttcgaatggt tagctgaagt ctttaaaaat aacctggcat ttgatggtca cggtaacgtt
     2281 gctttcttcg ttattatttt cctcagcatc atcgtgcgtt atttcttcgc ttccggtagt
     2341 gcctatatcg ttgctatgtt accggtattt gccatgctgg cgaacgtctc tggcgcaccg
     2401 ttaatgttaa ccgcgctggc actgttgttc tccaactcct atggcggcat ggttactcac
     2461 tatggcggcg cggcaggtcc ggtcatcttt ggcgtgggtt ataacgatat taaatcctgg
     2521 tggttggtcg gtgcggtact gacgatatta accttcctgg tgcatatcac cctcggagtg
     2581 tggtggtgga atatgctgat cggctggaac atgctgtaaa tatactcgtc atacttcaag
     2641 ttgcatgtgc tgagtctgcg ttcgctcacc ccagtcactt acttatgtaa gctcctgggg
     2701 attcactcgc ttgtcgcctt cctgcaactc gaattattta gagtatatcc atttattatc
     2761 tttctgcgca cttcacggtg cgcagatatc tggagcattt aatgatcaag ttatctgaaa
     2821 aaggcgtgtt tctcgccagt aataacgaaa taattgccga agaacatttc accggccaaa
     2881 ttaaaaaaga agaagccaaa aaaggcacta ttgcctggtc tattctctct tctcataata
     2941 cgtccggaaa tatggataaa cttaaaatta agtttgattc attagcctct cacgatatta
     3001 cctttgttgg tattgtacag accgctaaag cgtccggtat ggaacgtttc ccgctgccgt
     3061 atgtgctgac caactgccat aactcacttt gcgccgtcgg cggcaccatt aacggtgatg
     3121 accatgtttt tggtttatcg gcggctcagc gttatggcgg tatttttgtg cctccgcata
     3181 ttgcggtcat ccatcaatat atgcgtgaga tgatggcagg tggcggcaaa atgatcctcg
     3241 ggtcagacag tcacacccgt tacggtgcat tagggacaat ggcagtcggt gagggcggcg
     3301 gcgagttggt aaaacaattg cttaatgaca cttgggatat cgactatccg ggagttgttg
     3361 cggtgcatct gaccggaaaa ccagcgccgt atgtggggcc gcaggatgtg gcgttggcta
     3421 tcatcggtgc cgtgttcaaa aacggctacg tcaaaaacaa agtgatggaa ttcgtaggtc
     3481 ccggcgttgc tgcgctctct accgatttcc gtaacagcgt tgacgttatg accactgaaa
     3541 cgacctgttt aagttctgtc tggcaaaccg atgaagaagc ccataactgg ctggcgctcc
     3601 acggtcgcgg ccaggattac tgccagctta accctcaacc gatggcgtac tacgatggct
     3661 gcatcagcgt tgatttaagc gccatcaaac caatgattgc gctgccgttc cacccgagca
     3721 acgtgtatga aatcgacacg ctgaaccaga acctgaccga cattctgcgt gagattgaaa
     3781 ttgagtccga acgcgtggcg cacggtaaag ccaaactctc gctgctggac aaagtggaaa
     3841 atggtcgcct gaaagtgcag caggggatta tcgcgggctg ttctggcggt aactatgaaa
     3901 acgttatcgc cgcagcaaat gcgctgcgcg gtcaatcctg tggtaacgac accttctcgt
     3961 tagcggttta cccgtcatca cagccagtat ttatggattt agccaaaaaa ggtgtggtag
     4021 cagatttgat tggcgcaggc gcaatcatca gaaccgcgtt ctgcggccca tgctttggcg
     4081 cgggcgatac gccaatcaac aacggtttga gtattcgcca caccacgcgt aacttcccga
     4141 accgcgaagg ctctaagcca gctaatgggc agatgtcagc ggtggcgttg atggacgctc
     4201 gttctatcgc tgcgactgcg gcaaacggtg gctatttaac ttctgccagc gaactcgatt
     4261 gctgggacaa cgtgccggag tacgccttcg atgtaacgcc gtataaaaac cgtgtttatc
     4321 agggctttgt taaaggggca actcagcaac cgctgattta cggaccgaac attaaagact
     4381 ggccggaatt aggtgcgctg actgacaata tcgtcctcaa agtgtgctcg aagatcctcg
     4441 acgaagtgac caccaccgac gaactgatcc cttccggtga aacctcttct tatcgttcaa
     4501 atccgattgg tctggcggag tttaccctgt cacgccgcga tccaggttat gttggcagaa
     4561 gtaaagcgac tgctgagctg gaaaatcagc gtctggcggg gaatgtcagc gagctgacag
     4621 aggtgtttgc gcgcattaag cagattgctg gtcaggagca tattgatccg ctgcaaactg
     4681 aaattggcag catggtctat gcggtgaaac caggcgatgg ttctgcgcgt gaacaggcgg
     4741 cgagttgtca gcgtgtgatt ggcggtctgg cgaatattgc cgaggagtac gcgactaaac
     4801 gctatcgttc taacgtcatc aactggggga tgttacctct gcagatggcg gaagtgccaa
     4861 cctttgaagt gggggattac atttacatcc ctggcattaa agcggcgctg gataatccgg
     4921 gtacgacgtt taaaggttat gtgatccatg aagatgcgcc ggtaacggaa attacgctct
     4981 atatggaaag tctgactgct gaagagcgcg agattatcaa ggcgggtagt ttgattaact
     5041 tcaataaaaa ccgtcagatg taaaaagcgc catgtgaatg taggtcgcat tcggcactta
     5101 ttgtcggatg cgatgctggc gcatcttatc cgacctacaa atcgcatcga accgtaggcc
     5161 ggataaggcg tttacgccgc attcggcaaa tagttaatag ctcttacttc ttcgcctctg
     5221 caaccacttt gctacccacg ccgcggttat tgtattccca catgcggttg tagttagtgt
     5281 cattcagatt gcgctgtatt tcgtcgctat cgtcaacgct gccggtgtta cccgcaaacg
     5341 gacgattaga gatcaccgca tcgccccacg gtttagccgt gttaaaacct tcgttgatgg
     5401 cgctatcacg gatcaccacc tgaccgttgg tattggcatc aacatccagc gagcggccca
     5461 gttgtgccac gccatcaccg gaagcattga aacggctgtt tacggcgagg aaaccgtagt
     5521 aaatgttgga cagcgtagcc ggtgcaaaca catacgcttc ttgctgagta cgtgagttca
     5581 ccacgcggaa ttcggtgtta tcgaacacca ctgcgccgcg accagaaacg atatccacat
     5641 ccccttcaat gtagctgttg gtcaccagcg tacgcggctg acgattcgtt tccagacggt
     5701 tctgcacacc gctgttggtg acaaagaagg tgttctgacg accgagaatg ttaacgttgt
     5761 taatctgtac ctggtcaccg tcagtacgca gtgccaccgc cggatggtta cccgcatcta
     5821 cgctatcacc cagcgtgttt tcgatggtca ggttttgcag ttgcaggcca ttgttttgtg
     5881 accagaagac cgcagaacac agaacaccga tactgtcgct gcgtttgctc tggcagctat
     5941 cgtacatata ccacgctggt ttacctggca tatatttgcc gcgcgggttg acgtcgtgac
     6001 gccagtcggc agggctcata cccccatcaa gggaaagccc aatcttcaca tcaatcggtt
     6061 tttcacctgt accgtacaga gtaattccac ccggagcggc agggacatac accgttccct
     6121 gatactcacc aggcatcacg gcaatatact ggcgcttgtt ggtacgcttg ataattgccg
     6181 catctaccgc cgcctgaatc gtggtatgcg ttacaccttg agtacccgcc gggccgacaa
     6241 caaagtcagg ttgcgcaggc agggttatcg gggacggatt ccacgctgca gcacctggtg
     6301 tcagggatgc aaaatagtgt tgcgcatcga aattctgcgc ttcttttgcc gacagaatcg
     6361 ggcgagaaga ggtaccaggc gcagtttgat cagaaggacg ttgatcgggt ggtgttgagc
     6421 tacaggcggt cagcgtcacg ccaaaagcca atgccagcgc cagacgggaa actgaaaatg
     6481 tgttcacagg ttgctccggg ctatgaaata gaaaaatgaa tccgttgaag cctgcttttt
     6541 tatactaact tgagcgaaac gggaaggtaa aaagacaaaa agttgttttt aataccttta
     6601 agtgatacca gatagcattg cgccatctgg cagagtgatt aactaaacat cgcggtaatc
     6661 gatgcgcttg ccagagagtg gaaatgaacg ttaaacccga ccatcgcgcc gctggctcct
     6721 tcatcgacat caatacgctc tacatccagc gcgtgaacgg taaaaatgta gcgatgggtt
     6781 tcgcctttcg gcggcgctgc gccatcgtat ccggttttac caaaatcggt acgcgtctgc
     6841 aaaacgccgt ctggcattgc taccagacca gagccaaacc cttgcggtaa tacgcgggta
     6901 tcagcgggta aattaacaac tacccagtgc caccagccgg agccggttgg cgcatccggg
     6961 tcgtagcagg tgacaacaaa acttttcgtt cccgcaggaa catcatccca cgccagatgc
     7021 ggtgaaatat tatcgccatc gtaacccatg ccgttaaaga catgacgatg cggcaactta
     7081 tcgccatcgc gcagatcgtt actgatgagt ttcatgaacc ctcctttcta gtttgcagaa
     7141 agtgtagcca gaaaccctca cgctgacttc ccgttattgg caaaaaaatg tttcatcctg
     7201 taccgcgcgg ttaaccgctg cggtcagacg ctgcaactgt tgcgggagaa taatataggg
     7261 cggcatcagg taaatcagtt tgccaaaagg ccggatccag acaccctgtt cgacaaagaa
     7321 tttttgcagc gccgccatat tcaccggacg agtggtttcg accacaccga ttgcccccag
     7381 tacgcgcaca tcggcaacca tttcggcatc acgggctggt gccagttgct cgcgtagctg
     7441 tgcttcaata gccgctacct gttgctgcca gtcgccagat tcgagaatcg ccaggctggc
     7501 gttagctgcc gcgcaggcca gcggattacc cataaaagtt ggcccgtgca taaagcagcc
     7561 ggcttcaccg ttactgatgg tttctgcaac ctcgcgcgtg gtgagtgtgg cggaaagggt
     7621 cattgtgccg ccagttaagg ctttaccgag gcacaaaatg tccggcgcga tttctgcata
     7681 ttcacaggca aacagtttgc cggtacgacc aaatccggtg gcgatctcgt cggcaatcag
     7741 caagatacct tcgcgatcgc acattttgcg gattcgtttt aaccattccg gatggtagat
     7801 gcgcatcccg cctgcgccct ggacaatcgg ttcaatgatc accgccgcga tttcatgacg
     7861 atgcgccgcc atcaggcggg caaagcccac catatcgcgc tcatcccatt caccatccat
     7921 gcggctttgc ggggcgggag caaacaagtt ttctggcaga tagcctttcc acaggctgtg
     7981 cattgagttg tccggatcgc acaccgacat cgcgccaaag gtatcgccat gataaccatt
     8041 gcggaaggtc agaaaacgct ggcgcgcttc gcctttggcc tgccagtact gcaacgccat
     8101 tttcatcgcc acttccaccg ctacggagcc ggagtccgcg agaaaaacgc attccagcgg
     8161 ttgcggcgtc atcgccacca gtttgcggca cagctcaatg gctggcgcat gggtgatacc
     8221 gccaaacatc acatgcgaca tggcatcaat ttgcgacttc atcgccgcat taagctgcgg
     8281 gtgattgtaa ccgtggatcg ccgcccacca ggacgacata ccgtcaacca ggcgtctgcc
     8341 gtcagacaaa attagctcgc aaccttcggc gctcgccacc ggataaaccg gtagcgggga
     8401 ggtcatggat gtgtatgggt gccagatatg gcgttggtca aaggcaagat cgtccgttgt
     8461 cataatcgac ttgtaaacca aattgaaaag atttaggttt acgagtctac accgaattaa
     8521 caacaaaaaa cacgttttgg agaagcccca tggctcaccg cccacgctgg acattgtcgc
     8581 aagtcacaga attatttgaa aaaccgttgc tggatctgct gtttgaagcg cagcaggtgc
     8641 atcgccagca tttcgatcct cgtcaggtgc aggtcagcac gttgctgtcg attaagaccg
     8701 gagcttgtcc ggaagattgc aaatactgcc cgcaaagctc gcgctacaaa accgggctgg
     8761 aagccgagcg gttgatggaa gttgaacagg tgctggagtc ggcgcgcaaa gcgaaagcgg
     8821 caggatcgac gcgcttctgt atgggcgcgg cgtggaagaa tccccacgaa cgcgatatgc
     8881 catacctgga acaaatggtg cagggagtaa aagcgatggg gctggaggcg tgtatgacgc
     8941 tgggtacgtt gagtgaatct caggcgcagc gccttgcgaa tgccgggctg gattactaca
     9001 accataacct cgacacctcg ccggagtttt acggcaatat catcaccaca cgtacttatc
     9061 aggaacgcct tgatacgctg gaaaaagtgc gcgatgccgg gatcaaagtc tgctcaggtg
     9121 gcattgtggg tttaggcgaa acggtaaaag atcgcgccgg attattactg caactggcaa
     9181 acctgccgac gccgccggaa agcgtgccaa tcaacatgct ggtgaaggtg aaaggtactc
     9241 cactggcaga taacgatgat gtcgatgcct ttgattttat tcgcaccatt gccgtagcgc
     9301 ggatcatgat gccgacctct tacgtgcgcc tttctgccgg acgtgagcag atgaatgaac
     9361 agactcaggc aatgtgcttt atggctggcg caaactcgat tttctacggt tgcaaactgc
     9421 taaccacgcc gaatccggaa gaagataaag acctgcaact gtttcgcaaa ctggggctaa
     9481 atccgcagca aactgccgtg ctggcgggcg ataacgaaca acagcagcgt ctggaacagg
     9541 cactgatgac cccggacact gacgaatatt acaacgcggc agcactatga gctggcagga
     9601 taaaatcaac gcggcgctcg atgcgcggcg tgctgccgat gccctgcgtc gccgttatcc
     9661 agtggcgcaa ggagccggac gctggctggt ggcggacgat cgccagtatc tgaacttttc
     9721 cagtaacgat tatttaggtt taagccatca tccgcaaatt atccgtgcct ggaagcagag
     9781 tgcggagcaa tttggcgtcg gtagcggcgg ttccggtcac gtcagcggtt atagcgtggc
     9841 gcatcaggcg ctggaagaag aactggccga gtggctgggc tattcacggg cactgctgtt
     9901 tatctctggt tttgccgcta atcaggcggt cattgccgcg atgatggcga aagaggaccg
     9961 tattgttgcc gaccggctta gccatgcctc attgctggag gctgccagtt taagcccgtc
    10021 gcagcttcgc cgttttatcc ataacgatgt cgctcatctg gcgcgactgc ttgcttcccc
    10081 ctgtccgggg cagcaactgg tggtgacaga aggcgtgttc agcatggacg gcgatagtgc
    10141 gccactggcg gaaatccagc aggtaacgca acagcacaat ggctggttga tggtcgatga
    10201 tgcccacggc acgggcgtta tcggggagca ggggcgcggc acctgctggt tgcaaaaggt
    10261 aaaaccagaa ttgctggtag tgacttttgg caaaggattt ggcgtcagcg gggcagcggt
    10321 gctttgctcc agtacggtgg cggattatct gctgcaattc gcccgccatc ttatctacag
    10381 caccagtatg ccgcccgctc aggcgcaggc attacgtgcg tcactggcgg tcattcgcag
    10441 tgatgagggt gatgcacggc gcgaaaaact ggcggcactc attacgcgtt ttcgtgccgg
    10501 agtacaggat ttgccgttta cgcttgctga ttcatgcagc gccatccagc cattgatcgt
    10561 cggtgataac agccgtgcgt tacaactggc agaaaaactg cgccagcaag gctgctgggt
    10621 cacggcgatt cgcccgccaa ccgtacccgc tggtactgcg cgactgcgct taacactaac
    10681 ctccgcgcat gaaatgcagg atatcgaccg tctgctggag gtgctgcatg gcaacggtta
    10741 ataaacaagc cattgcagcg gcatttggtc gggcagccgc gcactatgag caacacgcag
    10801 agttacagcg ccagagtgct gacgtcttac tggcaatgct cccacagcgt aaatacaccc
    10861 acgtactgga tgcgggttgt ggacctggct ggatgagccg tcattggcgg gaacgtcacg
    10921 tgcaggtgac ggcgttagat ctctcgccgc caatgcttat tcaggcacgc cagaaggatg
    10981 cagcagacca ttatctggcg ggagatatcg aatccctgcc gttagcgact gcgacgttcg
    11041 atcttgcatg gagcaatctc gcggtgcagt ggtgcggtaa tttatccacg gcactccgcg
    11101 agctgtatcg ggtggtgcac cccggaggcg tggtcgcgtt taccacgctg gtgcagggat
    11161 cgttacccga actgcatcag gcgtggcagg cggtggacga gcgcccgcat gctaatcgct
    11221 ttttaccgcc agatgaaatc gaacagtcgc tgaacggcgt gcattatcaa catcgtattc
    11281 agcccatcac actatggttt gatgatgcgc tcagtgccat gcgttcgctg aaaggcatcg
    11341 gtgccacgca tcttcatgaa gggcgcgacc agcgaatatt aacgcgttca cagttgcagc
    11401 gattgcaact ggcctggccg caacaacagg ggcgatatcc tctgacgtat catctttttt
    11461 tgggagtgat tgttcgtgag taaacgttat tttgtcaccg gaacggatac cgaagtgggg
    11521 aaaactgtcg ccagttgtgc acttttacaa gccgcaaagg cagcaggcta tcggacggca
    11581 ggttataaac cggtcgcctc tggcagcgaa aagaccccgg agggtttacg taatagtgac
    11641 gcgctggcgt tacagcgcaa cagcagcctg cagctggatt acgcaacggt aaatccttac
    11701 accttcgcag aacccacctc accgcacatc atcagcgcgc aagagggcag gccgatagaa
    11761 tcatcggtaa tgagcgccgg attacgcgcg cttgaacaac aggctgactg ggtgttagtg
    11821 gaaggcgctg gcggctggtt tacgcccctt tctgacactt tcacttttgc ggattgggta
    11881 acacaggaac aactgccggt aatactggtg gttggcgtga aacttggctg tattaatcac
    11941 gcgatgttga cggcacaggc gatacaacac gccgggctga ctctggcggg ttgggtggcg
    12001 aacgatgtca cgcctccggg aaaacgtcac gctgaatata tgaccacgct cacccgcatg
    12061 attcccgcgc cgctgctggg agagatcccc tggcttgcag aaaatccaga aaatgcggca
    12121 accggaaagt acatagacct tgccttgttg tagctatcct gtatttggtt aaattgcgag
    12181 cgagatcgcg tcttcgattg actgcaattt aaccaattaa attctaaaat aatcacgaaa
    12241 aaaattttac ttccgcatca tgcggcgaat gtgggaattg cccaggcggc gggggatagg
    12301 ggctgcagac agttatccac tattcctgtg gataaccatg tgtattagag ttagaaaaca
    12361 cgaggcaagc gagagaatac gcggcttgca cgcgaattgg cgttaaagac ggctcaaaga
    12421 aatatctttt attttttaac tggttagata attg
//
LOCUS       DAZUCE010000149        12268 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZUCE010000149 DAZUCE010000000
VERSION     DAZUCE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.149"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..3700)
                     /gene="rhsB"
                     /locus_tag="ACHY03_003480"
     CDS             complement(<1..3700)
                     /gene="rhsB"
                     /locus_tag="ACHY03_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFS3906670.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQASSGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPDNLPAAPLVRYGWTPRGELAAVYDRSNTQVR
                     SFTYDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYCYEQNRITITDS
                     LDRREVLHTQGEGGLKRVVKKEHADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVT
                     GLITRITTPDGRASAFYYNHHSQLTSATGPDGLELRREYDESGRLIQETAPDGDITRY
                     RYDNPHSDLPCATDDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHRE
                     EGLSQYRAYDSRGQLIAVKDMQGHEMRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAI
                     RTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAERWRYDERGWLTDISHISEGHRVTV
                     HYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYG
                     SGWLAGMKLGDTPLVEYTRDRLHRETLRSFGHYELTTAYTPAGQLQSQHLNSLQYDRD
                     YTWNDNGELIRISSPRHTRSYSYSNSGRLTGVHTTTSTLDIRIPYATDPAGNRLPDPE
                     LHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQ
                     HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWD
                     GDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELARTQRRSLADALQQSGGEDGGS
                     VVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVKQMQNQMDPVYTPARKIHLY
                     HCDHRGLPLALISTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYY
                     NRHRYYDPLQGRYITQDPIGLKGGWNLYT"
     gene            complement(3860..4072)
                     /gene="rpmE"
                     /locus_tag="ACHY03_003481"
     CDS             complement(3860..4072)
                     /gene="rpmE"
                     /locus_tag="ACHY03_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFS3906671.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            4275..6473
                     /gene="priA"
                     /locus_tag="ACHY03_003482"
     CDS             4275..6473
                     /gene="priA"
                     /locus_tag="ACHY03_003482"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFS3906672.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGHAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKDLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYRGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            6629..7654
                     /gene="cytR"
                     /locus_tag="ACHY03_003483"
     CDS             6629..7654
                     /gene="cytR"
                     /locus_tag="ACHY03_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFS3906673.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            7746..8705
                     /gene="ftsN"
                     /locus_tag="ACHY03_003484"
     CDS             7746..8705
                     /gene="ftsN"
                     /locus_tag="ACHY03_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFS3906674.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            8798..9328
                     /gene="hslV"
                     /locus_tag="ACHY03_003485"
     CDS             8798..9328
                     /gene="hslV"
                     /locus_tag="ACHY03_003485"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFS3906675.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            9338..10669
                     /gene="hslU"
                     /locus_tag="ACHY03_003486"
     CDS             9338..10669
                     /gene="hslU"
                     /locus_tag="ACHY03_003486"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFS3906676.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            10736..11662
                     /gene="menA"
                     /locus_tag="ACHY03_003487"
     CDS             10736..11662
                     /gene="menA"
                     /locus_tag="ACHY03_003487"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFS3906677.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            11755..12240
                     /gene="rraA"
                     /locus_tag="ACHY03_003488"
     CDS             11755..12240
                     /gene="rraA"
                     /locus_tag="ACHY03_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFS3906678.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
BASE COUNT         2670 a         3379 c         3483 g         2736 t
ORIGIN      
        1 atgtatacag gttccatccc cctttcagcc caatcggatc ctgagtgata taccgcccct
       61 gcaacgggtc ataatagcgg tggcggttgt aatacaggcc ggactcctca tcatactgct
      121 gacccggcag gcggatgagc tgctgcagct gatgcgggtt ctcttcattc agcaggttgc
      181 cccactcatc gtattctgcg caccattctg tagccccttc cgtgctgata agcgccagcg
      241 gcaggccgcg atggtcgcag tggtacaggt ggatttttcg cgccggcgtg tacaccgggt
      301 ccatctggtt ttgcatctgc ttcaccgtca ggccgcacga tgccagccag cggcggcttt
      361 cctcactcac ccggtcagcc aggatttcac tttccagccg gtcgagcatc tgcaccagca
      421 ccggcgggaa caccacactg ccaccgtctt caccgccgga ctgctgaagg gcatccgcca
      481 ggctgcggcg ctgcgttctg gccagttcac cggtggcagt ttcaactctg atgagtggtg
      541 tgaagctccc cggctgataa atcgtctgga tgcgggttct gtcgttctgt atcgtggtca
      601 gccggtcgcc atcccagccg taccaggtca cttgcggttt ccgtgacagc gacatccagc
      661 ccgtcaggtc ccgttcacgt cgccataccc gttttgccac cctgcggccc agcgggtcgt
      721 aaagatagcg gctttcgacc agcggctctg catattgtgt ccgcgtgtag tgcaccagcc
      781 ggtgctgact gtcgtaatgg taccggtggg tgcgctcatc atccgtgcgg ataacccctt
      841 ccgggatgag gtcggttttc tctgtcagtc tgccgtgacg gtcataccgg taaagatagt
      901 gcgcgtcacg ggcgatacgg ttatccggcc acatgctgag ggtgctgtcc gggtgcagct
      961 ccgggtcggg caggcggtta cctgccgggt ccgtggcata cgggatgcgg atatccagag
     1021 ttgaggtggt ggtgtgaacg ccggtcagcc tgccggagtt gctgtagctg taactccggg
     1081 tatggcgcgg gctgctgatg cggatgagtt cgccgttgtc gttccaggtg taatcgcggt
     1141 catactgcag gctgttcagg tgctggctct gtaactgccc ggcaggggta taagcggtgg
     1201 tgagttcata atggccgaag ctgcgcagcg tttcccggtg caggcggtcg cgggtgtact
     1261 ccaccagcgg tgtgtcgccg agtttcatgc ctgccagcca gccgctgccg taggtcagcc
     1321 attccacggc gggcaggctg tccggtatac agcggttcgc cagcccctgc gcgttgtaag
     1381 cgtgtctggt ctcatgctgc cagagcagtg cttccgtctg cgggtgatgc accgtctgac
     1441 gctcaccggt cagccggcct ttctcatcat acccgtaatg caccgtcacc cggtgccctt
     1501 cgctgatatg gctgatgtct gtcagccagc cgcgttcgtc gtaccgccac cgctctgcgg
     1561 tttcgccatt cacggtgcgg tgcgtgaggc ggtctgcttc gtcatagtgc cagtgggtga
     1621 ccagcccctc atcctcgctg cggataagtt tgccggtcag gtcgtggtga taacgctgtg
     1681 tgcggccgtc aaagccggtt tcctgtatca gccggtcgag tacatcgtaa cggaaggtgg
     1741 tatggctgcc gttttcactg gtcaggcgga tgacccgtcc ggcagcatcg tattccatac
     1801 tgcgcgtcag accgccctgc gtggtacgga tggcctttcc ccacgcatcg tactgtgtcc
     1861 cgtttctgct gccgtccgga gcaatgacgg cggtcaggtc accggcggcg ttgtattcat
     1921 accgcatttc atggccctgc atgtctttca cggcaattaa ctgtccacgg ctgtcgtatg
     1981 cgcggtactg actcagccct tcctcgcggt gcaccgccgt catctgcccg aagcggtcat
     2041 ggtcataacg ggttacataa ccggaacagt cggtgaagct cagcaactgg ccgtaacggc
     2101 tccacgtcat ggttttccgg ctgccggtgg catcgtccgt tgcgcagggt aagtcactgt
     2161 gtggattatc ataacggtag cgggtgatat cgccgtcagg ggcggtttcc tgaatcagac
     2221 ggccggattc atcatattcc cggcgcaatt ccagcccgtc aggcccggtg gctgacgtta
     2281 actggctgtg gtggttatag taaaatgccg atgccctgcc atccggtgtg gtgatgcgcg
     2341 tgatgagtcc cgtcaccaca tccgggctgt attctgttgt ccggcctgcg gcatccgtct
     2401 gtgccttaag cctgcccacc gcatcaaact gactctgcgt gacgctgccg tccgcgtgtt
     2461 cctttttcac cacccgcttc agcccgcctt cgccctgcgt gtgcagcact tcacggcggt
     2521 ccaggctgtc ggtgatggtg atgcggtttt gctcataaca gtaggtgtag cttaagcccg
     2581 ccgggttaag ctgctctgtc acccgcccgt cgctgtcgta acggtaacgg atttccggtc
     2641 ggcccgtgtg acggtgcgcc accatccggc cccggtattt atcatcgtaa gtaaagctgc
     2701 gcacctgtgt attgctgcgg tcatacaccg ccgccagttc gccgcgtggc gtccagccat
     2761 agcgcaccag cggcgcggct ggcaggttgt ccgggtattc cgggtcgtgc gtcagccaca
     2821 cggcagacag acggatgccg ttgtcccggc catattcggt gtaacccggc agggtatcag
     2881 gaaaagcgga cggttccgtc ccgccggaag aggcctgctg ccgggcttct tctgcccgct
     2941 gcgcctgcgt ggtcagtacc agccggaagt gacgcccggc accatccgtc acgccggtga
     3001 tttcgccgct gaattcaccg gcggcttcgc ggtggaacgt ctgtgtgcgc ccgaagcggt
     3061 ccaccagccc ggtcagtacc cggtacggcg gcagcggcgc aggcagcacc tcatccgctt
     3121 ccggcacccg ctcacaccag ccgagcagcc accacggccc ctgcggactg tttgtcgcca
     3181 gataacgatg cggacttaag cggagttctt ccggcagcgc ctgccagagt gcggccagcc
     3241 ggtgaccttc atccagtttc gccacgccgc cgcgcaccag ccagagtgac tcgctgcggc
     3301 tgtaaccgtc ctcaccggga aacaggtgct caaaatacag gcttctgccg ccgttatcac
     3361 tgagtatcag tgtgttatcg cgcagctgta agcggatatc cgcaggcatt ttccagccgg
     3421 ggccgaggct ccccaccggc gcgggcgttt ttgtccggta actgctgtag gtgcgggaga
     3481 gaatgaacgg cagcggggcg ggcagggcga tgtcggtttc accgggaagg acctttgcac
     3541 cgagcagcgg attgaccgga tggccggacg tcactccgcc ggggcacacc gaacaggcca
     3601 cgccggtggg ggcaccgatg cgcaccccgg ctgaaccctg gacaatgcta ccgccatact
     3661 gcgtcatgtc gccctgacgc gccgccggtt ttccgctcat cgtaaaatcc ttttgaaata
     3721 aataagaacc agaagcctag cgccagtcaa caacaggaaa tgataatgag ttaacacccc
     3781 ggaatttttc agaatttata aaaggcaaat tacaggcaca aaaaaagcgc cgtgcggcgc
     3841 ttttttcgta aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt
     3901 caacacggcc accggtagca acatcacgct gtttgccagt gaagaacggg tggcacttgc
     3961 tgcacacgtc gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc
     4021 aagagcagct agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac
     4081 ctcggtttaa ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa
     4141 gtatagcttc aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc
     4201 gtatgcaaac tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa
     4261 gtcaggatga tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct
     4321 ttgactatct gctgccagaa ggcatgacgg ttaaagccgg gtgtcgcgtg cgcgtgccgt
     4381 ttggcaaaca gcaggagcgc atcgggattg tggtatcagt tagcgatgcc agcgaactgc
     4441 cgctcaatga gctaaaagcg gtagtcgaag tgctggatag tgagccggtg tttactcact
     4501 ccgtctggcg attgctgctc tgggcggcag attactatca tcatccgatt ggcgatgtgc
     4561 tgtttcatgc cttgccgatt ttactgcgcc aggggcggcc tgcggcgaac gcgccgatgt
     4621 ggtactggtt tgccactgaa caaggccatg cggtggatct gaacagcctg aaacgttcgc
     4681 caaagcaaca acaggcactg gcggcattgc ggcaaggcaa aatctggcgc gaccaggttg
     4741 ccacgcttga atttaatgat gccgcgttgc aggcgctgcg caaaaaagat ctgtgtgatt
     4801 tagcaagtga aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgaac
     4861 ggttgcgatt gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcggata
     4921 ccttctctgc ctggctgctg gcgggcgtta caggttccgg taaaacagag gtttatttga
     4981 gcgtactgga aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg
     5041 gcctgacacc gcaaactatc gcccgttttc gtgaacgttt taatgccccc gtagaagttc
     5101 tgcattccgg cctgaacgac agcgagcgtc tttcggcgtg gttgaaagcg aaaaatggtg
     5161 aggcggcgat tgtgattggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg
     5221 tgattgtcat tgatgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc
     5281 atgcccgcga cctggcggtt tatcgtgcgc acagcgagca aatcccgatt attcttggct
     5341 ccgcaacacc cgcgctggaa acgttgtgca acgtccaaca gaaaaaatac cgcctgctgc
     5401 gcctgacccg tcgggcaggg aatgcgcgtc cggcaattca acatgtgctg gatttaaaag
     5461 gtcagaaggt gcaggcgggg ctggctccgg cgttaatcac ccgtatgcgc cagcatttac
     5521 aggccgacaa ccaggttatc ctctttctta accgccgtgg ctttgcgcct gccttgctgt
     5581 gccacgactg cggctggatt gccgaatgtc cacgttgcga tcactactac acgctgcatc
     5641 aggcgcaaca gcatttgcgc tgtcaccact gtgacagcca acgcccggtg ccgcgccagt
     5701 gcccttcctg cggttccacg cacctggtac ccgtgggact gggcaccgaa cagcttgaac
     5761 agacgctcgc gccgttattc cctggtgtgc ccatttctcg tatcgaccgc gataccacca
     5821 gccgcaaagg agcgctggaa caacaactgg cggaagtgca tcgcggcggc gcgaggatct
     5881 tgattggcac gcaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtagcgt
     5941 tactggatgt ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgttttgccc
     6001 agctatacac ccaggtagca ggccgtgccg ggcgtgcggg taaacagggc gaagtggtgc
     6061 tgcaaacgca tcatccggaa cacccgctat tgcaaacgtt gctctataga ggctacgacg
     6121 cttttgccga acaggcactg gctgagcgac gcatgatgca gctgccgccg tggaccagcc
     6181 atgtgattgt gcgtgcggaa gatcataaca atcagcatgc gccgctgttc ctgcaacaac
     6241 tgcgtaatct gatcctctcc agcccgctgg cagacgacaa attgtgggtt ctcggtccgg
     6301 ttccggcact ggcacctaaa cgtggcggtc gctggcgctg gcagatattg ttgcagcacc
     6361 cctcccgcgt gcgcttgcaa cacatcatta acggtacgct ggcactcatc aatacaatac
     6421 cggattcccg taaggtgaaa tgggtgctgg atgttgatcc gattgagggt taagccgctc
     6481 acgatgcgag gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa
     6541 ccgttttcac gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta
     6601 cgcctgacgt gccaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac
     6661 catgaaagac gttgccctca aggcaaaagt ctctacagcg accgtctccc gagcattaat
     6721 gaatcccgat aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt
     6781 gggttattta ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct
     6841 ggtgattgtc ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatagaagt
     6901 tacggcggca aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca
     6961 ggaaaaaacc tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg
     7021 ttcaaggctg ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat
     7081 ggcgaacgaa tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc
     7141 cgcatttgat gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc
     7201 cggtcccgaa gagatgccgc tgtgtcacta ccgcctgcaa ggttacgttc aggcgctgcg
     7261 tcgctgcggc attatggttg atccgcaata catcgcccgt ggcgacttca ccttcgaagc
     7321 cggaagcaaa gcgatgcagc agctgcttga tcttccacaa ccgcctactg ctgtcttctg
     7381 ccatagcgat gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt
     7441 cccggaagac ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc
     7501 gccgctgaca accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt
     7561 gcttgatcaa atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact
     7621 tatcatccgg ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact
     7681 ggtcaaaggc ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa
     7741 cgatagtggc acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa
     7801 agagcacctc acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta
     7861 ttgctgccgc cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga
     7921 aagaagagtc cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac
     7981 cagaagaacg ctggcgctac atcaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc
     8041 ccacagaacc ttctgctggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac
     8101 gtcagcttct tgaacaaatg caggctgata tgcgccagca gccaacgcag ctggttgaag
     8161 ttccgtggaa tgaacagacg ccggaacagc gccagcaaac gctacagcgc cagcgtcagg
     8221 cgcagcaatt agcggaacag caacgtctgg ctcagcagtc gcgtacgact gaacaaagct
     8281 ggcagcagca gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta
     8341 aaccggcttc tactcagcag ccttaccagg atctgctgca aacccctgcg cacacgaccg
     8401 cgcagtcgaa gccgcagcaa gctgcgccag ttgctcgtgc cgctgacgcg ccaaaaccga
     8461 cggcggagaa aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg
     8521 aacaggcaga gacggtacgt gctcagctgg cgttcgaagg atttgactcg aaaatcacca
     8581 ccaacaatgg ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca
     8641 gcaccctcaa tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg
     8701 gttgaaaccc tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg
     8761 ggtttgtact ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg
     8821 ccgtaacggc catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat
     8881 gaaaggcaac gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc
     8941 gggcggtact gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca
     9001 tcagggccat ctggttaaag ccgccgttga gctggcaaaa gactggcgta ccgatcgcat
     9061 gctgcgcaaa cttgaagcac tgctggcagt cgcggatgaa actgcatcgc ttatcatcac
     9121 cggtaacggt gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc
     9181 ttacgcccag gctgcggcgc gtgcactgtt agaaaatact gaacttagtg ctcgtgaaat
     9241 tgctgaaaag gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac
     9301 catcgaagaa ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga
     9361 aatcgtcagc gaactggata agcacatcat cggccaggac aacgccaagc gttccgtggc
     9421 gattgctctg cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt
     9481 gaccccgaaa aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg
     9541 tcgtctggct aagctggcga atgcgccgtt catcaaagtt gaagcgacca aattcaccga
     9601 agtgggctat gtcggtaagg aagtggattc tattattcgc gatctgaccg atgccgccgt
     9661 gaaaatggta cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga
     9721 agaacgtatt ctcgacgtgc tgatcccacc ggctaaaaac aactggggac agaccgaaca
     9781 acagcaggaa ccgtccgctg ctcgtcaggc attccgcaag aaactgcgtg aaggccagct
     9841 tgatgacaaa gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc
     9901 tcctccaggc atggaagaga tgaccagcca gctccagtcc atgttccaga acctgggcgg
     9961 ccagaagcaa aaagcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga
    10021 agaagcggcg aaactggtga acccggaaga actgaagcaa gacgctatcg acgctgttga
    10081 gcagcacggg atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc
    10141 cggtccggat gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg
    10201 caccgtttcc accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg
    10261 cgcgttccag attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc gtctgccaat
    10321 ccgcgttgag ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa
    10381 tgcctctatc accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt
    10441 taccgactcc ggtattaagc gcatcgcgga agcggcatgg caggtgaacg aatctaccga
    10501 aaacatcggt gctcgtcgtt tacataccgt tctggagcgt ttaatggaag agatttccta
    10561 cgacgccagc gatttaagcg gtcaaaatat cactattgac gcagattatg tgagcaaaca
    10621 tctggatgcg ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat
    10681 cattttcatc attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac
    10741 tgaacaacaa attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc
    10801 cctcgccttt gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcattttga
    10861 tccgctggtc gccctgctgg cactgattac cgccgggcta ttacagatcc tttctaacct
    10921 cgccaatgat tacggcgatg cggtaaaagg cagcgataaa cctgaccgta tcggaccgct
    10981 acgcggcatg caaaaagggg tcattaccca gcaagagatg aaacgggcgc tcattattac
    11041 cgtcgtgctc atctgtctct ccgggctggc actggttgca gtggcgtgcc atacgctggc
    11101 cgattttgtc ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac
    11161 cgtgggcaat cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt
    11221 tggctggtta agcgtcatgg ggagctggta tttacaggct catacattga ttccggcact
    11281 gatccttccg gcgaccgcat gcggcctgct ggcaacggca gtactgaaca ttaataacct
    11341 gcgtgatatc aatagcgacc gcgaaaacgg caaaaacacg ctggtggtgc gcttaggtga
    11401 agtgaacgcg cgtcgttatc atgcctgcct gctgatgggc tctctggtat gtctggcgct
    11461 gtttaatctc ttttcgctgc atagcctgtg gggctggctg ttcctgctgg cggcaccgtt
    11521 gctggtgaag caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccgat
    11581 gctggaacgc actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt
    11641 cctaagccag tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac
    11701 agaccacata gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa
    11761 tacgatactt ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg
    11821 ttctccaact ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc
    11881 gaggacaacg ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc
    11941 gtcgatggcg gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca
    12001 gtacaaaatg aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg
    12061 gaagagttgg atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa
    12121 ggcattggcg aaagcgatgt ccgcgtcaat tttggcggtg ttaccttctt ctccggcgac
    12181 catctttatg ccgacaatac cgggattatt ctttcagaag atccgctgga tattgaataa
    12241 gtatctgcca tgcgttaaaa ggggaatt
//
LOCUS       DAZUCE010000150        12070 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZUCE010000150 DAZUCE010000000
VERSION     DAZUCE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.150"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            17..550
                     /locus_tag="ACHY03_003489"
     CDS             17..550
                     /locus_tag="ACHY03_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS3906679.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATQVDGKPVVPGMKLSKEKCDQVNAIERDKVLAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            841..972
                     /locus_tag="ACHY03_003490"
     CDS             841..972
                     /locus_tag="ACHY03_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906680.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            936..1090
                     /locus_tag="ACHY03_003491"
                     /pseudo
     CDS             936..1090
                     /locus_tag="ACHY03_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1155..1616
                     /locus_tag="ACHY03_003492"
     CDS             1155..1616
                     /locus_tag="ACHY03_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS3906681.1"
                     /translation="MNRILYVVIIVLLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREAPTTSGVDNATAPRLADTVERDYFTLRERLMLMQKQLEGAQDYIRTQC"
     gene            1916..2722
                     /locus_tag="ACHY03_003493"
     CDS             1916..2722
                     /locus_tag="ACHY03_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFS3906682.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            2703..4409
                     /locus_tag="ACHY03_003494"
     CDS             2703..4409
                     /locus_tag="ACHY03_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFS3906683.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            4409..6553
                     /locus_tag="ACHY03_003495"
     CDS             4409..6553
                     /locus_tag="ACHY03_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFS3906684.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEAAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            6712..7719
                     /locus_tag="ACHY03_003496"
     CDS             6712..7719
                     /locus_tag="ACHY03_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906685.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            7743..8957
                     /locus_tag="ACHY03_003497"
     CDS             7743..8957
                     /locus_tag="ACHY03_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFS3906686.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            9013..9402
                     /locus_tag="ACHY03_003498"
     CDS             9013..9402
                     /locus_tag="ACHY03_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906687.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            9452..9913
                     /locus_tag="ACHY03_003499"
     CDS             9452..9913
                     /locus_tag="ACHY03_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906688.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRHRVAETEQHGGTE"
     gene            9897..10460
                     /locus_tag="ACHY03_003500"
     CDS             9897..10460
                     /locus_tag="ACHY03_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906689.1"
                     /translation="MAELSDFLPYVRRQISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            10460..11110
                     /locus_tag="ACHY03_003501"
     CDS             10460..11110
                     /locus_tag="ACHY03_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFS3906690.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            11107..>12070
                     /locus_tag="ACHY03_003502"
     CDS             11107..>12070
                     /locus_tag="ACHY03_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFS3906691.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKSASDSAK
                     TATSRAAEAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAEKARQHAEKA
                     RLAQESAGEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGP
                     QGPAGPKGERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGD"
BASE COUNT         3085 a         2786 c         3472 g         2727 t
ORIGIN      
        1 cggggagagt aagctgatga gcaggaaatt ccgctatggt ttatcagcgg tcgttctggc
       61 gctgattgct gcaggtgctt ctgcgcctga aatcctcgac cagtttcttg acgaaaaaga
      121 aggtaaccac accacggcat accgtgatgg tgcgggtatc tggaccatct gccgtggtgc
      181 cacccaggtg gatggtaagc ctgtcgtccc cggcatgaag ttgtcgaagg aaaaatgcga
      241 ccaggttaac gccattgaac gtgataaggt gctggcatgg gtggcgaaaa acatcagagt
      301 gccactgacc gaacctcaga aagcggggat cgcgtcattc tgtccttaca acattggccc
      361 cggtaagtgt ttcccgtcga cgttttataa acgaattaat gcaggcgatc gcaggggagc
      421 gtgtgaagcg attcgctggt ggattaagga cggtggcaga gactgccgta tccgctcaaa
      481 taattgctac ggtcaggtat cccgtcgcga ccaggagagt gcgctggcat gttggggtat
      541 cgacaggtag cagaatattt tgctgaaaaa taacgttggt caacgcggac ggataacacg
      601 aaatcctgcg aactggcaaa acctaagtga ataaaagtaa aaaccccgtt tttggcagca
      661 agcggggttt tgtgtttatg gcagtaagct atgggtggct gccttgattg attttagcaa
      721 actgattagg gagttgcgac tcatgattag tcaattacca aactggaagt ttttgctggt
      781 ctggagcatc ccttttttat ggggggtatc ccagttaatt gtggcaatta aggggtagct
      841 atgtcagaca aactcataac gctggcgaag atcctctgtg taatcgtcgg catttcattt
      901 tcactaatgc tggttgctat ttttcttttc ctgccctgga tgatgttgtc ttcgtcgggg
      961 atgctggggt aacagtgact gatgacatca gcagagcgct ggctttgcta ttaagtgggt
     1021 ggcggttggt atcgctgtgt ctccgatact gtatgggctg gcaaaactgg tcattgcgct
     1081 gaaatcgtga actttattta aacagatgag taatgaaatt gctgtactgg tccgggcaat
     1141 tcgctggtgg tgatatgaac cgtattctgt atgtggtgat tattgtactg ctggtggcct
     1201 gtggtgcgct tagtctgggg ctgaatcatt accgcgataa cgccatcacc tacaaagcgc
     1261 agcgcgataa aaaagccaga gagctggagc tagcaaacgc aaccattact gacatgcagc
     1321 agcgccagcg tgatgttgct gcgcttgatg ccagatactc aagggaatta gccgatgcga
     1381 gagctgaaaa tgaaactctg cgcgctgatg ttgccgctgg tcgtaagcgc ctgcggatca
     1441 acgccacctg ctccggtacc gtgcgtgaag cccccaccac ctccggcgtg gataatgcaa
     1501 ccgccccccg actggcagac acagttgaac gggattattt cactctcaga gagcggttga
     1561 tgctgatgca gaagcagctg gaaggggcac aggactatat ccgcactcag tgctgaacta
     1621 agttttgctg atgcgccgta tcgtcgccgt attcccgcat aaacagagac cgcagccacc
     1681 ttatctgcgt gagtgtgcgg gggtaatcaa aaacgatgca caccgggttt tctcattttt
     1741 cacgagatgg gagcgatttc ccgcgaagcc gcctgtccgg tgcggtggtg gaagaaaccg
     1801 gataaaacaa cctcattgta caaatatcga tcaagtatgg cgctgctgtg tgaattctga
     1861 aaaatcacag cggtcattat gcgtcagttt ttaacagagg acgtcagaaa gtgacatggc
     1921 aaagctggac tggaaaaagc tggagcaggc attccgacgc gaacatgccg aaacgggaat
     1981 aacattactg gactggtgcc ggaagaaaaa gattaattac aacaccgcca gaacccgtat
     2041 aaaaatgggc aaaatcgatc atgaaattga tcataaaacc gatcatgaaa tcgatcatga
     2101 catctcagat gaagaaccca gcaatgacgc gtgttccgac gatgaaaaat gcgcaaaaaa
     2161 ctctgaaaaa aactgcgcaa attcggcaga aacgaaacgg attcgtggtt cccgactttt
     2221 acctccttca aacgcttttt ctcagcgaaa cacccacgcc gtaagacacc gtggatatgc
     2281 gaagtatctt gaggcagata acctcatgga tgatgcgtcc gacatggtgc tgttcgatga
     2341 actggtgttc acccgggccc gcgcactttc agtaactaag gcacttaaag ggatgttcgc
     2401 cgacctggaa gaggcaactg acgtggaaac ccgcgttgct ctttacgaca aaatactcaa
     2461 agctgaacag gcccttgacc ggaatattgc ccgtatcgag tcaattgaac gctcattgct
     2521 gacgctggac gtcctggctg agacagcacc aaaacttcgt gctgaccggg aaagaatcaa
     2581 cgccgccaga gataaactca gagcggaaac cgatattctg accagccagc gccggggtgt
     2641 tattacgcca gtcagtgaca tcgtgtcatc gctgcatgaa atgagtaatt cggggagact
     2701 ggatgacatt ccggaagaat aaaacgcgat gtgatgaacc tgcagaaatg actgagaccg
     2761 aacaacgtct gttcattatg acaaaactga gcaatccctg gtggcgcctc aatcatctct
     2821 acaaaataca gaacgaaaaa ggtgaactgg tcaccttcag aatgcgaccg gcgcaacgtc
     2881 agttgtttcg taacatgcat aataaaaaca ttatcctgaa agcgcgccag ctgggatttt
     2941 ccacagccat tgatatttat cttctcgacc aggcattatt cattccgcat ctcaaatgcg
     3001 ggatcgtcgc tcaggataaa caggctgcca gtgaaatttt ccgcacaaaa attgctgtac
     3061 cgtttgatca tctccctgac tggctgagag cctcattcac catcgttgaa cgtcgtagcg
     3121 gtgccagcgg tggctatatc ctgtttggtc acggctcgag tattcaggtg gcaacctcat
     3181 ttcgctcagg tacggtgcag cgcctgcata tctcagagca cggcaaaatt tgcgcgaaat
     3241 atccggctaa ggcgaaagag ctgcgaaccg gtacgcttaa tgccgtctct gatgaatgca
     3301 ttatttttga tgaatccacg gctgaaggcg tgggtggtga tttttacgag atgagtaacc
     3361 gtgcacagga gagcactgca tcaggcttat tgctgacggc acaggattat aaattccatt
     3421 tttacgcatg gtggcaggat cctaaataca gcgccagagt gcctgaaagc gggctgaagc
     3481 tgtcacggga aaaaatgacg tatttttctg cggttgagaa ggcaatgaac atcacgctta
     3541 ccgatgagca gaagcagtgg tacatcagta aggaaactga acagcgtgag gaaatgaagc
     3601 aggagtttcc ctcaacgcca caggaggcgt ttctgacgtc cggacgacgt gtgttcagtg
     3661 ccgaaagtac attgcaggca gaatcattct gttcgccacc gatgattgtt tatgacattg
     3721 aacctgttac aggagcgaag actaaagctc agtctctgcg tgaaggaaat aaaaacgagt
     3781 tgcagcggac gctgatgaat tatctgctgg tatgggaact gccggatccg gatgaagagt
     3841 atgtttgtgg ggcagatact gccgaagggc tggagcacgg agaccgctca tcgctggatg
     3901 ttgtcaaacg cagtaatggc gagcaggtgg ctcactggtt cgggcatctc gatgctgaac
     3961 tttttgctca tctcatttcg caggtctgtc gtatgtataa caacgcgttt gtggggccgg
     4021 agcgtaataa tcacggacat gcagttatcc tgaaactccg ggaactctat ccgacacgtt
     4081 atatctacaa cgaacagcat cttgaccagg catatgacga cgatacgccc cgccttggct
     4141 ggctgacaac ccgtcagagc aaacctgttc tgaccgaagg aatgaaaacg ctcctgaata
     4201 atggaatatc agggatccgc tggtcaggca cattatcgga aatgaacacc tacgtttatg
     4261 acgcgaaagg ctccatgaat gcacaggaag gctgctttga tgatcagctc atgagctaca
     4321 tgattgccca ggagatgcgc gccagaatgc cggtgagggt aaaacagaaa acggataaac
     4381 gcagaaccac acactggatg gcacactgat gaaaaatgaa attaacacca cagcgatgaa
     4441 aaacgatcat ggatccacgc cgcgtttttc tcagcgtcag ttactgtctc tctgttctga
     4501 tattgacagt cagcctctct ggcgtgatgc cgcaaacaag gcctgtgcgt attatgatgg
     4561 cgaccagctg gcaccggaag ttatccaggt actgaaagat cgcggtcagc ccatgaccat
     4621 ccataacctc attgccccca cggtagatgg tgtactggga atggaggcaa aaacaagaac
     4681 ggacctgata gtgatgtcag acgatccgaa cgatgaaaca gagaaactgg cagaggccat
     4741 taatgcggag tttgctgatg cgtgccgtct tggcaacatg aataaggccc gctccgatgc
     4801 ctatgcggaa caaatcaagg cggggctcag ttgggtggag gtcaggcgca acagcgatcc
     4861 gttcggacct gaattcaggg tatccacagt cagcagaaat gaggttttct gggactggct
     4921 cagccgggaa tccgacctga gtgactgccg gtggctgatg cgtcgccgct ggatggatac
     4981 cgatgaggca aaagccacgt tcccgggaat ggctcaggtt atcgattatg ctattgatga
     5041 ctggcgtggt tttgtcgata ccacgattac tgaaggccag cccagtccgt tgatgagtgc
     5101 atgggaagag tatcagtcat gggatcgcag ggagaatgaa tggcttcagc gtgaacgccg
     5161 tcgtgtgctg cttcaggtgg tttattaccg tacattcgag cgtcttccgg tgattgaact
     5221 cagtaatgga cgggtggtgg cttttgataa aaataatctg atgcaggcgg tggctgtggc
     5281 atccgggcgg gttcaggtga aagtcgggcg ggtaagtcgt attcgtgaag cctggtttgt
     5341 gggcccacac tttattgtgg atcgcccctg tagtgcaccg caggggatgt ttccgctggt
     5401 tcctttctgg ggataccgaa aggataaaac cggggagcca tacgggttaa tttcccgcgc
     5461 cattccggca caggatgagg tgaattttcg tcgtatcaag ctgacctggc tgcttcaggc
     5521 caaacgcgtg attatggacg aggatgccac ccagttgtca gacagcgacc tgatggagca
     5581 gatcgaacgt ccggatggca ttattaaact gaatccggcc cgaaaaaatc agaaaagtgt
     5641 cgcagatgtt tttcgggttg agcaggattt tcaggttgcc agccagcagt ttcaggtcat
     5701 gcaggaatcg gaaaaactta tccaggatac catgggagtg tattccgcat ttctcgggca
     5761 ggattcaggt gcgacgtcag gcgtggctat cagtaacctg gtggagcagg gggccacaac
     5821 ccttgcggaa atcaacgata actaccagtt tgcctgccag caggtgggaa gactgttgct
     5881 ggcttatctt ctcgatgacc tgaaaaaacg ccgtaatcat gcagtggtga ttaatcgcga
     5941 tgatcgccag cgtcgccaga ccattgtcct caatgctgaa ggtgataatg gtgaactgac
     6001 caatgatatt tcaaggttaa atacacatat tgcgctggcg cctgttcagc agacaccggc
     6061 gtttaaggca cagcttgcac agagaatgtc agaggttatt caggggctgc cgcctcaggt
     6121 gcaggctgtt gtgctcgacc tgtgggttaa tcttctggat gtgccgcaga aacaggagtt
     6181 tgttgagcgt attcgtgctg cgctggggac gccaaaatca ccggatgaaa tgacgccgga
     6241 agaacaggaa gcagcggcac aacaacaggc acttcagcaa caacaggcag aactccagat
     6301 gcgcgagatg gctggcagag tggcaaaact ggaagctgac gccgccaggg cacatgcagc
     6361 tgcacaacgg gataatgcca gtgcacagcg ggaagtcgcc ctgacacagg ggcagcgtta
     6421 tgtggatgcg cttaaccagg cacatacggc agaaatcatt accggcgtac agaatatgga
     6481 acaggagcag gacgttcttc agcaacagat gctgtatacg ttacaacagc ggatgaatga
     6541 aatgtcgctc tgaaaactct ggcttcaact gaaccccgtc atcgtacggg gttttttgtt
     6601 tccggaggta agcgttccgg gagcggtgcg cttattcgcg ggggcagcga taagccttat
     6661 tttactcaac cattcggatc tgtccgataa acagaccatg cggagttatt tatggatttt
     6721 gaatttacgg gtgaagaaac cccggaacaa ctggaaaaaa tgctggaagg acttggggat
     6781 gtggatattg acagtcacgc acaggacgtc gtgacggaag ataccacgga aaaacatgcg
     6841 gatgaggaag cacagactca gacgggcgat aacaatgtgg caccgacgcc ggatgccagt
     6901 gtggagcaga cgcaggacgt gaaggagccg gaagcgaagg gggtgctcac ccgcgacggt
     6961 aaacacgtca ttccctatga agtccttgag gctgaacgtt ccggtaagca acgggccgaa
     7021 caggaagccg cacttcttcg tgggcagata gctgaagaaa aacgcagggt ggaactgctg
     7081 acgtctcaga tccaccaggc cggtatgaag cccacaccgt taccggaaaa cgaaaaaatt
     7141 tctgatgagc agattgcccg tatcagggag atgtatccgg aaattggtga cgcggtggct
     7201 tcgctcatcc gtaaaaataa ctatctccag tcccgtgttc agcaatcaac acagcaggca
     7261 gaaggtaatg gtggtgagga tttatcaccg gttcttgatg cgatgaatgc cgtgccggtg
     7321 ctgaaaacgt ggcaggagtc cgatccggat cgcttctcgg ttgctgtatc catcgacggg
     7381 aagctccaga atgaccccgc atggaaagac aaaacgctca ctgaacgttt cgctgaagtg
     7441 gcccgtcgta cgcaggttgc tttcggtgaa gtcagtgagt cgtctgctga caaccaggca
     7501 gacaaaacgg atatccggaa aacggcggaa gaaaaagtga agaccgctga acaggagcag
     7561 gcagtacctg cttccccgtc agatttaggc accacggcct ccgtcggaac cggtgataat
     7621 tttgaacggt tacttggtgc ttctcattca gaggctgagg cgattatgcg tggtatgacg
     7681 aatgctgaaa tagacgcact tctggagaag ctcgggtaac ttactgaagg agtactgaag
     7741 taatgacgac tgtaacatca gcccaggcga ataagctgta tcaggtggcg ctttttactg
     7801 ctgccaaccg caaccgctcg atggttaata tccttactga acagcaggaa gcgccaaaag
     7861 cggtttcgcc ggacaagaaa agcacgaagc agaccagcgc aggtgcgccg gttgtccgta
     7921 tcacagacct taacaaacag gccggtgatg aagtgacctt cagcatcatg cacaaactct
     7981 caaaacgtcc gacgatggga gatgagcgtg ttgaaggtcg tggtgaagac cttagccatg
     8041 ctgatttttc cctgaaaatt aatcagggac gtcacctggt ggatgcaggc ggacgtatga
     8101 gtcagcagcg cacgaagttt aacctggcgt cgtctgccag aacgcttctg gggacgtact
     8161 ttaatgacct gcaggaccag tgtgcgatag tgcatcttgc tggtgctcgc ggtgattttg
     8221 ttgctgacga caccattctg ccgacagcgg agcaccctga attcaaaaaa atcatgatca
     8281 acgatgtact gcctccgaca catgaccgtc acttttttgg cggtgatgcg acaagctttg
     8341 agcagattga agcggcagat attttttcta ttggcctggt ggacaatctc tccctgttca
     8401 ttgacgaaat ggcgcatccg ttacagccgg ttcgtctgtc cggtgatgaa cttcacggag
     8461 aagatccata ttacgtcctg tacgtcacgc cgcgtcagtg gaatgactgg tacacctcga
     8521 cgtccggtaa ggactggaac cagatgatgg ttcgggccgt gaaccgtgca aaaggtttta
     8581 atcatccgct gttcaaaggt gaatgtgcga tgtggcgcaa tatcttggtt cgtaagtatg
     8641 cgggtatgcc gatccgtttc tatcaggggt caaaggttct ggtatcagag aataacctga
     8701 cggcaaccac gaaagaggtc gctgctgcaa ccaatattga ccgcgccatg ttactggggg
     8761 ctcaggcgct ggcaaatgct tacggtcaga aggcgggcgg tcacttcaac atggttgaga
     8821 agaaaacgga tatggataac cgtactgaga tagcaatcag ctggatcaac ggtctgaaaa
     8881 aaatccgttt ccccgagaag agcggcaaga tgcaggatca cggcgtgatt gccgttgata
     8941 cagcagtgaa gctctgattt tttcctttcc ccatgccggg ttttcgcccg gctttttcag
     9001 gagtcattaa ttatggcaaa gactatcctt gccccgtcac tgagtgaacg ggtctatacg
     9061 ggtacgcacg gtaatgagtc ggtggcagaa ggcgtattta cggtgaatgc tgcggaagcg
     9121 gacagtgtta ttcatcttct ctcactgcca gtgggcatcc gtatcaactc actccagctg
     9181 gtttcaacgg gtggtctggg tactgcaacc gtcagcatta agtccggtga gcatgctctc
     9241 atcgataaca gcgaagctgt ttctgcaaaa tttgccagat atgtgccagt ggagccgtac
     9301 accacacagc gtgacgggga gctggttact gtcaccatta agactgccgc tgcaaccggc
     9361 accctgaatg ttctgctgcg ttataccgtg gtgggatact gattaaaacc ttccggcccg
     9421 cgtcatgcgg gctttttatc cggggaatta tatgagtgag aaaattgccg ttgtctatat
     9481 cggcccaaaa cccgtgaaaa aggacaccat taccggaagc cgcacattgt tcccacgtct
     9541 tgagccggtg catgttgaca gtgcgatggc ctggcaactg ctggggtttc cggatgtctg
     9601 ggttcgtcat gaagagcttg atgatgttct gaaaaagcaa caacagaatg agcagttgcg
     9661 gcaggcacag caggcgcagg aaagagtgct tgctgcgcgg gcagaagcgg agaacagttt
     9721 tgttgtttct gttaacgggc aggaggtgga tttaagtaag ctcacctcag cacggctggc
     9781 gacgctgtgt gaggcagaag agctggatat tcacaaagat ccgaaagaaa cggctgaggc
     9841 attccgtatc cgggtgcgtg aggcatttcg ccatcgtgtt gcggagactg aacagcatgg
     9901 cggaactgag tgatttttta ccgtatgtcc gtcgtcagat cagcggtcca ctgaacatta
     9961 tgatgacgga tgctctgtca atggctgccg tggcattcag ccgtcagtcg ctggtgtgcc
    10021 gtcgggaggt tactgttgta ccagtagcag gaaaagaaat cgtgcttccg tatgacaaag
    10081 atgatgagga gtgcgttcat atcatccgta tctctgacga taatcatgag ctttttgtcg
    10141 gtcgggatgt ggatatcagc tccggacgct ccctgcgatt tgcctgttct cccggtgagg
    10201 tgagcgtgct ttatgccgtc gctccgaaag ccggacgcag ccagataccg gatgaactcc
    10261 tcacatggcc tgaagaagtg gctgcggggg cacttgagcg gttgttcatg cagactggtg
    10321 tttcatggtc agatccgtta cgcgcacagt atttttctgt gcagttttct gaggggatcc
    10381 gtcgggcata tcgtcataca ctggcgacaa gcccgtactc ttcataccgc aaccctgtac
    10441 gcaggcagag gttttactga tgacgacgat tacagaaatc atcggacgtg tgaatacaca
    10501 actggttgac ccgatgatgg ttcgctggcc cctgcaggaa ttgtgcgatt attacaatga
    10561 tgctgtgagg gcagtgattc tggcgagacc ggatgctggc gcaagcctgg aaacaatcag
    10621 ttgtgttcct ggcgcccgtc aggttttgcc cgatggtgta atacaacttc ttgacgtgat
    10681 atgcctcagt gacggtagtg cagtcagacc attatcccgg gaggtgctgg atgcgcagta
    10741 tcctgagtgg cccacaatga agggcattcc tgaatgtttt atcagcaacg acctgtcccc
    10801 gcgcgtattc tggctgtttc ctgtccctga caaagagata agtattgatg cagtggtaag
    10861 ccggataccg gaggcagtgt atgttctgac gcaggacgat gatacgccag ttccactgga
    10921 agaggcttat gttaacccac tggtggactg gatgttgttt cgtgctttca gtaaggatgc
    10981 tgccggtggt gcagaatcgg ggctggctgc gcagcattat cagagttttg ttgagcaact
    11041 tgggatcaaa cagggggcag acagtgcatt gtctgcccgt aaaaaagtgt ttaacggagg
    11101 tggagtgtga gtgttgttgt ttcggggacg ctgaaatctc ctgatggtga ggcgatatca
    11161 ggagcaaata ttaccctgac ggcgctgaca gtttcaccgg atgcgctcag cggcaccagt
    11221 gcgtcggcag tgacccgtga aggtggatat tacggaatga cgatggatcc gggggagtat
    11281 gcggtttcgg tgacggtgaa agggaagact gctgtctacg gacgtgtgcg tattgagggg
    11341 accgaaagta cggtgacgct caatatgctg ttacgccgca gtcttgttga ggtgagcata
    11401 cccggagagc tgctgacaga tttccggcag atacagaata atgtggctga tgaccttgct
    11461 actattcgtc gcctgaatga agacacggcg acaaaaaaca ctcaggccac acagtcaaaa
    11521 gaaagtgcag cagccagtgc gaagagtgca tctgacagtg cgaagagtgc atctgacagt
    11581 gcaaagacgg caaccagcag ggcggctgaa gccggacaaa aagcgactga tgccactgag
    11641 gccgcgaccc gtgcaatcac agcagcgggg aatgcagagg aaagctcgac ccgtgccgga
    11701 gagtctgaaa aagccgccgg agctgatgca gaaaaagcca gacagcatgc tgaaaaggcc
    11761 aggctggcgc aggagagcgc cggagagatc cttaagcggg cagaggctgc cactgtcagt
    11821 gctgaagagg ccagacgtat ggctgagaat gcacgggggc cccgggggcc tcagggagaa
    11881 actggtccga agggggatgt cggtcctaaa ggcgaaacag gtccagtggg ccctcaaggg
    11941 cccgcagggc cgaaaggtga gcgtggtgac gttggtgctc agggggctgt agggcccgct
    12001 ggtccgcgtg gtgagaaggg cgaacagggg gagcgaggac cgcagggaat accaggcctg
    12061 aagggggata
//
LOCUS       DAZUCE010000151        11970 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZUCE010000151 DAZUCE010000000
VERSION     DAZUCE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.151"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1344)
                     /gene="uidB"
                     /locus_tag="ACHY03_003503"
     CDS             complement(<1..1344)
                     /gene="uidB"
                     /locus_tag="ACHY03_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFS3906692.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQ"
     gene            complement(1341..3152)
                     /gene="uidA"
                     /locus_tag="ACHY03_003504"
     CDS             complement(1341..3152)
                     /gene="uidA"
                     /locus_tag="ACHY03_003504"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFS3906693.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELYVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQRGGKQ"
     gene            complement(3543..4133)
                     /gene="uidR"
                     /locus_tag="ACHY03_003505"
     CDS             complement(3543..4133)
                     /gene="uidR"
                     /locus_tag="ACHY03_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFS3906694.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4362..5129)
                     /gene="hdhA"
                     /locus_tag="ACHY03_003506"
     CDS             complement(4362..5129)
                     /gene="hdhA"
                     /locus_tag="ACHY03_003506"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFS3906695.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5241..6269)
                     /gene="malI"
                     /locus_tag="ACHY03_003507"
     CDS             complement(5241..6269)
                     /gene="malI"
                     /locus_tag="ACHY03_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFS3906696.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKSIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6444..8036
                     /gene="malX"
                     /locus_tag="ACHY03_003508"
     CDS             6444..8036
                     /gene="malX"
                     /locus_tag="ACHY03_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFS3906697.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFIWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8046..9218
                     /gene="malY"
                     /locus_tag="ACHY03_003509"
     CDS             8046..9218
                     /gene="malY"
                     /locus_tag="ACHY03_003509"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFS3906698.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSCRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEKEKVAIMPGYTYGEEGLGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9322..10323
                     /gene="add"
                     /locus_tag="ACHY03_003510"
     CDS             9322..10323
                     /gene="add"
                     /locus_tag="ACHY03_003510"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFS3906699.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(10359..11399)
                     /gene="ydgJ"
                     /locus_tag="ACHY03_003511"
     CDS             complement(10359..11399)
                     /gene="ydgJ"
                     /locus_tag="ACHY03_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFS3906700.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11642..11767
                     /gene="blr"
                     /locus_tag="ACHY03_003512"
     CDS             11642..11767
                     /gene="blr"
                     /locus_tag="ACHY03_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFS3906701.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
BASE COUNT         2911 a         3140 c         2925 g         2994 t
ORIGIN      
        1 ctgcactttt ttacgattat caatttcaac cacgatttct ttgaattttt tatccgtgag
       61 cggataaaac cagataataa cgaatgccag tagcataaat ccgcaaggta ctaaggcaat
      121 tgatgtgcgg atgcccataa taacttccgg cgtttgcacc tgattggcga tatatccgct
      181 taaccccaaa ataaaggcag gaattgaacc tccgattgcc tgaccgcatt tacgggtaaa
      241 tgagaatagt gaataggtga gcccttcaat tcgcacgccg gtcaggtatt caccgtattc
      301 tacggtatca gcttccagcg cccacataac agtcatggta acgccctgac caattgaagc
      361 gatggccaac gcaaccaacg ccaccggcag tgaccagacg gaaacccaga agaatagcag
      421 ataaccgcag gttcccagca aagcgccaat caggaaggta ttctttttac cgatcctcgc
      481 gaccatcccc ggcaccagcg gtgccgatgc cacagtaccg accaggtttt gcaccagtac
      541 cagcacagtg aacagcccgg tatcatttaa cacatagcgc acgtagaaca acgacgaggc
      601 gctgaccgcg aaggtcgaaa tcagcacaca cagcgcaccg atgcacaaca taaacagcgg
      661 gcgattccgt ttcagggttt gcagactgat cttcaatgac ggctgcgcca cgatacgtac
      721 cacattctca cgcgtcgatt tgaagcagat gaagtaaagc accattccgg caatcgccag
      781 cacaatcgtc cagaaatggt ataccgacac catctcttcc gggctggagt tcttaatgct
      841 cggccctatc agaaatgcca ggcagacaaa ggtcaatgaa gcggcaatcc cacgagccgc
      901 gcccagacgg gcgcgggatt gtggttgttg ggtcatcgcg gtagcaagtg aaccataagg
      961 aatattcacc aggctgtagc aaagcccgag gcccatgtag gtcaaatatg catacaccac
     1021 tttgctacca tggctccagt cggtcggcac ccagaatacc agcacgctga agatcattaa
     1081 cggcgcagta ccgaagagta aaaacgggcg gaattttccc cagcgggtat tcacactgtc
     1141 caccactcgt ccggcaaaga cgtcggcgaa ggcatcgaat acccgcacca gtaacagcat
     1201 ggtgcccgcc gcagcggcac cgacgccagc gacgtcggtg tagtaactca acaggaagag
     1261 cgcccccatt gcgaaggcga agttattggc gacgtcaccg aggctgtagc cgacgatggt
     1321 gcgccaggag agttgttgat tcattgtttg cctccccgct gcggtttttc accgaagttc
     1381 atgccagtcc agcgtttttg aagcagaaaa gccgccgact tcggtttgcg gtcgcgggtg
     1441 aagatgccct tcttgttacc gccaacgcgc aatatgcctt gcgaggtcgc aaaatcggcg
     1501 aaattccata cctgttcacc gacgacggcg ctgacgcgat caaagacgcg gtgatacata
     1561 tccagccatg cacactgata ctcttcactc cacatgtcgg tgtacattga gtgcagcccg
     1621 gctaacgtat ccacaccgta ttcggtgatg ataatcggct gatgcagttt ctcctgccag
     1681 gccagaagtt ctttttccag taccttctct gccgtttcca aatcgccgct ttggacatac
     1741 catccgtaat aacggttcag gcacagcaca tcaaagagat cgctgatggt atcggtgtga
     1801 gcgtcgcaga acattacatt gacgcaggtg atcggacgcg tcgggtcgag tttacgcgtt
     1861 gcttccgcca gtggcgcgaa atattcccgt gcaccttgcg gacgggtatc cggttcgttg
     1921 gcaatactcc acatcaccac gcttgggtgg tttttgtcac gcgcaatcag ctctttaatc
     1981 gcctgtaagt gcgcctgctg agtttccccg ttgactgcct cttcgctgta cagttctttc
     2041 ggcttgttgc ccgcttcgaa accaatgcct aaagagaggt taaagccgac agctgcagtt
     2101 tcatcaatca ccacgatgcc atgttcatct gcccagtcga gcatctcttc ggcataaggg
     2161 taatgcgagg tacggtagga gttggcccca atccagtcca ttaatgcgtg atcgtgcacc
     2221 atcagcacgt tatcgaatcc tttgccgcgc aaatccgcat cttcatgacg gccaaagcca
     2281 gtaaagtaga acggtttgtg gttgatcagg aactgttcgc ccttcactgc cactgaccgg
     2341 atgccgacgc gcagcgggta gatatcacac tctgtctggc ttttggctgt gacgtacagt
     2401 tcatagagat aaccttcacc cggttgccag aggtgcggat tcaccacttg caaagtcccg
     2461 ctggtgcctt gtccagttgc aaccacctgt tgatccgcat cacgcagttc aacgctgaca
     2521 tcaccattgg ccaccacctg ccagtcaaca gacgcgtggt tacagtcttg cgcgacatgc
     2581 gtcaccacgg tgatatcgtc cacccaggtg ttcggcgtgg tgtagagcat tacgctgcga
     2641 tggatcccgg cgtagttaaa gaaatcatgg aagtaagact gctttttctt gccgttttcg
     2701 tcggtaatca ccattcccgg cgggatagtc tgccagttca gttcgttgtt cacacaaacg
     2761 gtgatacgta cacttttccc ggcaataaca tacggcgtga catcggcttc aaatggcgta
     2821 tagccgccct gatgctccat cacttcctga ttattgaccc acactttgcc gtaatgagtg
     2881 accgcatcga aacgcagcac gatacgctgg cctgcccaac ctttcggtat aaagacttcg
     2941 cgctgatacc agacgttgcc cacataatta cgaatatctg catcggcgaa ctgatcgtta
     3001 aaactgcctg gcacagcaat tgcccggctt tcttgtaacg cgctttccca ccaacgctga
     3061 tcaattccac agttttcgcg atccagactg aatgcccaca ggccgtcgag ttttttgatt
     3121 tcacgggttg gggtttctac aggacgtaac ataagggact cctcattaag ataaaaatac
     3181 tggtcaacct ttaatctgat tagttaacca attctgtgtt gcacacagtt atctgtgaga
     3241 aacccatgga agagaaatgg aaataaaaag aactgacacc gcgatcaaaa aaccaaaaac
     3301 tgcaataccc acagcgtgat atagatcgca ttaatcttta aaacggcgtt gacagaagcc
     3361 ataaaataag caaccgaacg ttcgttcgag cgtattggga acgataaact ctcatgacat
     3421 atggctacag tgaatatttt ggggggaatt ctccgaaccc gggaaggata atttttgcac
     3481 agagaaatta cgatatgaaa cgcgggcaca gttatcacca gcccgccgaa aaagagagag
     3541 aatcaggatg cggttaagat accgccaatc atcgcgcgta atccctgagc aaacgacgct
     3601 tcacgcgcca ggtcttccgc ttcgatatcg gccagtacac cgtaggtcag atccagcagt
     3661 aaacgtgaag tcattgccgt gttgatgtct gggcttatct cgcctttttg ctgggcatca
     3721 cgcatccgct gagcaacaaa ttcagtgatc gtcatatgct tatttttaag catggcggcg
     3781 acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgcccg ttgcccaaag
     3841 gcttcatggg tgagagagac aatagactcg accatatagt caacaaaatg aatcccttca
     3901 atcggttccc ggaaacgggc cagcgccctc tcctggtcct gtaagataat cgcctgaatc
     3961 aaggcttctt tggagatgaa atggtgatag agcgtcccgg gactaatggc gcaggattta
     4021 cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc tctggcagca
     4081 ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat cattaaacca
     4141 ttatagttag cgtgaataac tagtttaccg cgttgtcagg tgagcgccta tcattgctgt
     4201 tgaatagtgc gaaggcacac tctattcgta tgcttttcat ccagctattg gttaaccaat
     4261 agccgtgatt tggctgccag ttgtttagca gagacgttga tgaattgcac acgctatccc
     4321 ggtaaacaac acatacggct gtttaccggt tcgttagtgt attaattgag ctcctgtacc
     4381 ccaccaccgg agacggtgag aatttgtccg cttacccagc tggctgcagg cgagcaaagg
     4441 aacagcgctg cgttagcaat atcttgcggt tggcccagac gtctgatcgg cgtgtgctgt
     4501 aacatttttt gttcaatttc tggtgtaata acggatttca gggcatcggt taatattgcc
     4561 cccggcgcaa tgccattcac ccgaatattt ttttcaccca ggtcaaatgc catatttctg
     4621 accagatgac tggccgcagc tttagatgat gcataggaag tcatgtttat atttttattt
     4681 tctgccgcca tagaagtgat ggtcagaata acgccaccgc catttttttc catttctggc
     4741 gcaacaagtt gtgacagatg gaaaaaagaa aacacattca gttcataagc acggcgaaaa
     4801 tccgccattg gcatatcaaa cggtttaggt ccaccgccac cggcgttgtt aaccagaata
     4861 tcaactttac ccagcttact gatagcaaag tctgccagtg cagagagttc ctgttcggaa
     4921 gtaatatcac aacggcaggc aaatgcctga ccacccagtt gttgaatttc gtctacaaca
     4981 tggttagctg cgtcggcgtt aatatcactg accaccacag atgcgccagc tgtcgcgaat
     5041 gtaatggcga tttctttacc aatacctgca cccgcacctg tgatgatggc gcattttccg
     5101 tcgagtctca ggttgtcaga attaaacaca taaacctcct gtgaacttca gtaaaaataa
     5161 aaggcaccgc ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccagg
     5221 tatagcagat aaagagagga ttatttcgcc gcaatgagcc gggcgggaat aataagattg
     5281 cgtgaatgcg tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca
     5341 agttcccgcg caggcgtgct ggcccaggta acgggtatat catcaagtgt ggttggtgtc
     5401 gcatcggtaa atgccgccag cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc
     5461 ccgctttgcc gccctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta
     5521 tagcaaacca cggcactgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg
     5581 gcttgcttct ggctggaagt gcactccaac acccaatcgc tgtgaaacgg caggccaaat
     5641 tttagtagag ttgcacaata gccccccacc cgttctgcac gggttaatga ggaactttgc
     5701 cctcccagcc aggcgatccg ctgatgccca ttgcgaatga gatgctccgt caacaactgt
     5761 gcagcctgca tgttgtccgg gcgaaccgta tcaacatcat caagataact ggcacgggaa
     5821 gcgaaaatca ccgggataga tttttcttct gccatccgtc gcaggtcatc gctgcttcct
     5881 gcagccccgg caattaccac accatcgaca ccctgattca gtaacagtga aaaccgctgt
     5941 gccagctgct cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc
     6001 agagcttccg tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg
     6061 atcaaaccaa tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca
     6121 aatcccagct cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct
     6181 ttgccactaa gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca
     6241 tgaatggtta tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag
     6301 ataaaacgtt ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt
     6361 atgcctcact aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat
     6421 tgttctctac gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg
     6481 agttcttcca gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg
     6541 gcattatgct cggcattggt agttccctta gtagccacga tgtcatcacc ctaatcccgg
     6601 tcctgggcaa ccccgtgttg caggctatct ttatctggat gagtaagatt ggctcgtttg
     6661 cttttagttt cctgccagtc atgttctgta tcgccatccc gctgggtctg gcacgagaaa
     6721 acaaaggcgt agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa
     6781 acttctggtt gaccaataaa ggcattctgc caaccacgga tgccgccgtt ctgaaagcca
     6841 ataacatcca gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtaa
     6901 tcgccggtat tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg
     6961 cgctggcatt cttcggcggt acgcgcttcg taccaattat ctcctcgctg gtgatgggcc
     7021 ttgtcggcct ggtgattcca ttagtctggc cgattttcgc catgggtatt agcggcttag
     7081 gccatatgat caatagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc
     7141 tgctgttgcc gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag
     7201 gtggcacgca ggaagtctgc ggtcaaaccg tcagcggcgc attgaccatc ttccaggcgc
     7261 aattgagttg cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag
     7321 gcaaaatgcc tgcgtttctc ggcggtctgc caggtgcagc gttagcgatg tatcactgcg
     7381 cgcgcccgga aaatcgccat aaaattaaag ggctgctgat ttctggcctg atcgcctgtg
     7441 tcgttggcgg cacgaccgaa ccgctggaat tcctgttcct gttcgtagcg ccagttctgt
     7501 atgttatcca cgcgctgtta actggcctcg gcttcaccgt catgtctgtg ctcggcgtca
     7561 ccatcggtaa taccgacggc aatatcatcg actttgtggt gttcggtatt ttgcatggtc
     7621 tgtcaaccaa gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc gtttactacg
     7681 tcatcttccg tttcgctatc acccgcttca atctgaaaac cccggggcgc gatagcgaag
     7741 tggccagttc aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc
     7801 cggcaatcct cgaagcctta ggcggtgccg acaatattgt tagcctcgat aactgcatta
     7861 cccgtctgcg tttgtctgtg aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca
     7921 atcgggcaat tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac
     7981 aagttcagtc agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat
     8041 aagatatgtt cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acgcagtggg
     8101 attatgtcgc tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg
     8161 attttgccac tgccccctgc attatcgagg cgctgaatca gcgcctgatg cacggcgtgt
     8221 ttggctacag ccgctggaag aacgatgagt ttctcgcggc tattgcccac tggttttcca
     8281 cccagcatta caccgccatc gatcctcagt cggtggtgta tggcccttct gttatctata
     8341 tggtctcaga actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacac
     8401 ccgcctatga cgcattttac aaggccattg aaggtaatca gcgcacagta atgcccgttg
     8461 ctttagagaa gcaggctgac ggctggtttt gcgatatggg caagctggaa gccgtgttgg
     8521 cgaaaccaga atgtaaaatt atgctcctgt gtagcccaca gaatcctacc ggaaaagtgt
     8581 ggacgtgcga tgagctggag atcatggctg acctgtgcga gcgtcatggt gtgagggtta
     8641 tttccgatga aatccatatg gatatggttt ggggcgagca gccgcatatt ccctggagta
     8701 atgtggcgcg gggagactgg gcgttgctga cgtcgggatc gaaaagtttc aatattcccg
     8761 ccctgaccgg tgcttacggg attatagaaa atagcagttg tcgcgatgcc tatttatcgg
     8821 cactgaaagg acgtgatggg ctttcttccc cttcggtact ggcgttaact gctcatatcg
     8881 ccgcctatca gcaaggcgcg ccgtggctgg atgccttacg cgtctatctg aaagataacc
     8941 tgacgtatat cgcagataaa atgaacgccg cgtttcctga actcaactgg cagatcccgc
     9001 aatccactta tctggcctgg cttgatctac gtccattgaa tattgacgac aacgccttgc
     9061 aaaaagcgct tatcgagaaa gaaaaggtcg cgatcatgcc ggggtatacc tacggtgaag
     9121 aaggtcttgg ttttgtccgt ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg
     9181 tggctggatt aattaacgcc atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa
     9241 atcgttgcgc aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat
     9301 atccagaaaa agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc
     9361 caccttgatg gtaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc
     9421 tcgcttcctg cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa
     9481 cccgatctgg tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt
     9541 gatgcttgtc gccgcgtggc atttgaaaac attgaagatg cagcccgtca cggcctgcac
     9601 tatgtcgagc tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgttgca
     9661 ggtgttgttg aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag
     9721 gcgaagctta tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg
     9781 gaggcctttt tagcccaccg tgaccagatt accgcacttg atttagccgg tgatgaactt
     9841 ggtttcccgg gaagtctgtt tctttctcac ttcaaccgcg cgcgtgatgc gggctggcat
     9901 attaccgtcc atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa
     9961 ctgggggcag agcgcattgg acatggcgta aaagccattg aagatcgggc gctgatggat
    10021 tttctcgccg agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc
    10081 accgtggcag agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc
    10141 agcattaaca ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt
    10201 gccgcgccag ctgctgggtt atcccgcgag caaatccgcc aggcacagat taatggtctg
    10261 gaaatggctt tcctcaacgc agaggaaaaa cgcgcactgc gagaaaaagt cgcagcgaag
    10321 taacaaaaat ggatggtgca aattgcacca tccatttttc atgcaaggca caaagtcgcg
    10381 cgatgtttgg cggattcgat gcccagctca atcaactcca ttacctggat tgcctggctt
    10441 gccggaaccg gattttcacc atcgccattt aacgcatcac gaatcgccgc atagtaagcc
    10501 ggataattcc caggcaccgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg
    10561 gtaagtacgc catcacgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt
    10621 ttcagacgtt cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc
    10681 acgatatacc gtgctgactc agcggctgcc agcatggtac cgtgtaaaat gactcgccgc
    10741 tgtggatagg acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt
    10801 gccaaatcta ccgtcatgct gaccggtaaa ccaaatagcg taatggcctg atcaagaaga
    10861 tgtggtgcta aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg
    10921 cgcacctgcg gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg
    10981 ccttccgcga gcaaaccttt tagcgtcaag aaatcgctat cccaacgacg gttatggaat
    11041 acagacagca cacgccccag gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt
    11101 gtcacggtaa agggtttatc aacgaccaca tgtttacccg cctcaagcgc tgctttggct
    11161 aacgggaaat gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta
    11221 aacagatgct tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt
    11281 tcatcactgc tggagattac cgccagttcc agcccgggtg tgcccgcaat caggggcgca
    11341 tggaaggttt tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg
    11401 tcattgcctc tcattgaagt atgatggcta tttgacacta tcctttaccc tcgctcaaca
    11461 gtttaataac ttgccagcaa taaaggatgt tgtttaactt aagtcaaaaa aatagcgaat
    11521 tttccaacga caaaagctaa atatcgcaaa aacctcagta aaaatcttgc tggagctatt
    11581 attgctaagt aacatttacc ccctgaagtt aatggatcaa tcaacagaga tgtgggctgt
    11641 aatgaatcgt cttattgaat taacaggttg gatcgttctt gtcgtttcag tcattcttct
    11701 tggcgtggcg agtcacattg acaactatca gccacctgaa cagagtacta cggtacaaca
    11761 caagtaagct ctgcgcttgt agagcgacat gctgcccgtc cgggtgcatg ttttcacttg
    11821 tcggatatta aaccaggaat ttattatctt gttcgatgtt gttggtgatt gtcagggata
    11881 gtaaagcgcg ttactctttt cagagcagtt tttcatccct gaatatttgc catcagcgca
    11941 ttcttattat tgtaataaca caagcgttca
//
LOCUS       DAZUCE010000152        11888 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZUCE010000152 DAZUCE010000000
VERSION     DAZUCE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.152"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            12..614
                     /gene="yebB"
                     /locus_tag="ACHY03_003513"
     CDS             12..614
                     /gene="yebB"
                     /locus_tag="ACHY03_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFS3906702.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(616..1137)
                     /gene="ruvC"
                     /locus_tag="ACHY03_003514"
     CDS             complement(616..1137)
                     /gene="ruvC"
                     /locus_tag="ACHY03_003514"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFS3906703.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1172..1912)
                     /gene="yebC"
                     /locus_tag="ACHY03_003515"
     CDS             complement(1172..1912)
                     /gene="yebC"
                     /locus_tag="ACHY03_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS3906704.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1941..2393)
                     /gene="nudB"
                     /locus_tag="ACHY03_003516"
     CDS             complement(1941..2393)
                     /gene="nudB"
                     /locus_tag="ACHY03_003516"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFS3906705.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2511..4283)
                     /gene="aspS"
                     /locus_tag="ACHY03_003517"
     CDS             complement(2511..4283)
                     /gene="aspS"
                     /locus_tag="ACHY03_003517"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFS3906706.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4593..5159
                     /gene="yecD"
                     /locus_tag="ACHY03_003518"
     CDS             4593..5159
                     /gene="yecD"
                     /locus_tag="ACHY03_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFS3906707.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLHRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5156..5974
                     /gene="yecE"
                     /locus_tag="ACHY03_003519"
     CDS             5156..5974
                     /gene="yecE"
                     /locus_tag="ACHY03_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFS3906708.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6027..6422
                     /gene="yecN"
                     /locus_tag="ACHY03_003520"
     CDS             6027..6422
                     /gene="yecN"
                     /locus_tag="ACHY03_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFS3906709.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6463..7206
                     /gene="cmoA"
                     /locus_tag="ACHY03_003521"
     CDS             6463..7206
                     /gene="cmoA"
                     /locus_tag="ACHY03_003521"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFS3906710.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7203..8174
                     /gene="cmoB"
                     /locus_tag="ACHY03_003522"
     CDS             7203..8174
                     /gene="cmoB"
                     /locus_tag="ACHY03_003522"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFS3906711.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8339..10768)
                     /gene="torZ"
                     /locus_tag="ACHY03_003523"
     CDS             complement(8339..10768)
                     /gene="torZ"
                     /locus_tag="ACHY03_003523"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFS3906712.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(10793..>11888)
                     /gene="torY"
                     /locus_tag="ACHY03_003524"
     CDS             complement(10793..>11888)
                     /gene="torY"
                     /locus_tag="ACHY03_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFS3906713.1"
                     /translation="GKKRIGLLFLLIAVVVGGGGFLLAQKALHKTSDTAFCLSCHSMS
                     KPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDD
                     KFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGETCI
                     DCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTDLT
                     VVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYG
                     NQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIAKG
                     MGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2696 a         3167 c         2921 g         3104 t
ORIGIN      
        1 ggaatgacaa cgtgaatatt aattaccctg ctgaatatga aattggagat atcgtcttta
       61 catgtatagg tgctgcctta tttggtcaaa tatcagctgc atcaaattgc tggagtaatc
      121 acgtcgggat cattatcggt cataacggtg aagacttcct ggttgcagaa agccgggttc
      181 ccctttcaac catcaccacg ctatctcgtt ttattaaacg ttcgtctaat caacgctatg
      241 ctataaagcg attagacgct gggctgacag aacaacaaaa acaacgaatt gttgaacagg
      301 ttccctcccg gctacgcaaa ctttaccaca ccggttttaa atacgaatct tcgcgccagt
      361 tctgttcaaa atttgttttt gatatttata aagaagcgct atgtattccg gtgggtgaaa
      421 tagagacgtt tggagaattg ttaaatagca atccgaatgc aaaactcact ttctggaaat
      481 tctggttcct gggttctatt ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc
      541 atcatccggg tttggtgttg attcatgcgg agggagtgga aacgcctcag ccggaactga
      601 ccgaggcggt ataacttaac gcagtcgccc tctcgccagg ttcagccgcg attcgctcat
      661 ctgcatcgca ttctgactaa cgtggcagtg ggtgatggca atcgccagcg catcggcggc
      721 atccgcctgt ggattagcgg gcagtttcag caaggtgcgg accatatgct gcacctggct
      781 tttttcggca ctaccaatac ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa
      841 tactggcaac tcctgattca ccgccgccac aatcgccacg ccgcgcgcct ggcccagttt
      901 cagggctgag tcagcgttct ttgccataaa gacttgttca atggcgaaat aatcaggctg
      961 gaactgggtg atgatttccg tcacgcccgc atagatgagt ttcagacgag acggtaaatc
     1021 atccactttg gtgcggatgc atccgctacc caggtaggac agttgcctac ctacctggcg
     1081 gatgacgccg tagccggtca cgcgcgaacc cggatcaatg ccgagaataa tagccatcac
     1141 gcgtctccgt tttgctgttt agcaggcctc atcagagagt cgctgcgacc tcatcagaga
     1201 tttcaccgtt atggtaaact tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac
     1261 gcatcagttt cggtgcggtt tctgcatcca tatcagcttt ggtagacggg atcatggaaa
     1321 cttccgcgct gtctgctttc agacctgccg cttccagtgc gtcgcgcact ttacccattt
     1381 cttcccatgc agtgtagaca tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac
     1441 ctgcttccaa tgctgcttcc atgatggtgt cttcatcgcc tttctcgaag gagatcacgc
     1501 cttttttgct gaacagatag gctacggaac catcagtacc gaggttaccg ccacatttgc
     1561 tgaatgcatg acgcacttca gcaacggtac ggttgcggtt gtcagacaga cattcaatca
     1621 tgattgccgt gccgccagga ccgtaacctt cgtagatgat ggtttccatg ttcgcatcat
     1681 catcaccgcc cacaccacgt gcaattgcgc ggttcagcgt gtcacgggtc atgttgttag
     1741 acagtgcttt atcaattgct gcacgcagac gcgggttagc gtccggatcg ccaccgccca
     1801 acttcgccgc ggttaccagc tcacgaatga ttttagtgaa gattttaccg cgcttagcat
     1861 cctgcgcagc tttacgatgt ctggtgttgg cccatttact atgacctgcc ataaaaatat
     1921 ctccaaatag ccctgcctgt tcaggcagcg ttaattacaa actgttcaat cgcctgccgg
     1981 ttgctccagg acttagtgag cgccgccgca gcaggcgcat caagccactt gtaagccaga
     2041 tgttcagtga aaacgatctg ccgctcgtgc ggaagcgcaa gacagaacca tgattccgta
     2101 ttacgcgtca cgcccggcgc atagcgatga cgtaaatgtg aaaaaatttc aaactctacc
     2161 gtgcgctgac agtcaattaa ggtcagttgt tcagcgacaa catcaatggt gacctcttcc
     2221 tttacttcgc gcatggcagc ttgcggcgcg gtttcaccct cttccacgct gccggttacc
     2281 gactgccaga aatcggggtc atcacgccgc tgcaacatca gcacccgttt cgtatcttgt
     2341 gcgtagatga ccactaagat cgaaacggga cgcttataca ctttatcctt cacgctgcct
     2401 cttcgttgac tgccttcgct catcccattc acatagttat ctatgctcat gggagttcac
     2461 tcagttgccg cctcgatgca acgcgaatga tttcgtgtat ttgagtcata tcagttattc
     2521 tcagccttct tcacaacctg aatgctcagc tcagccagtg cagtcgggtt agcaaagctc
     2581 ggtgcttcag tcatcagaca cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg
     2641 atattgtcgg tgccggtcag cagcatggtc agacggtcaa gaccgaatgc cagacctgcg
     2701 tgcggcggag taccgtattt cagagcgtcg agaaggaagc cgaatttctc gcgctgttcc
     2761 tcttcgttga tacccagaat accaaacacc gtctgctgca tatcaccatt atggatacgt
     2821 actgaaccac cgcccacttc gtaaccattg atgaccatat cgtaagcgtt cgccaccgca
     2881 ttttccggtg cagctttcag ctctgcagcc gtcatgtctt tcggtgaggt gaacggatgg
     2941 tgcattgccg tcaggccgcc ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac
     3001 agcggtgccc atttgctttc gtcggtcaga ccaaggtctt tacccacttt caggcgcaat
     3061 gcgcccatcg cgtcggcaac aattttcttg ttgtcggcac cgaagaaaat catatcgcca
     3121 tcttgcgcgg cagtacgatc caggatggct tcgatgattt ctgcattaag gaacttcgct
     3181 accgggctgt tgataccttc cagacctttc gcgcgttcgt taactttgat gtaagccaaa
     3241 cctttcgcgc cgtagatttt aacgaagtta ccgtattcgt cgatctgctt acgggtcagc
     3301 gatgcgccac ccggaacacg cagagcggca acacggcctt tcggatcgtt cgccggacct
     3361 gcaaatactg caaactcaac agatttcagc agatcagcaa cgtcggtcag ttccatcggg
     3421 ttacgcagat ccggtttatc agaaccataa cggcgttcgg cttccgcaaa ggtcattacc
     3481 gggaaatcgc ccagatccac gcccttcact tccagccaca gatggcgcac cagcgcttcc
     3541 atcacttcac gcacttgcgg cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat
     3601 tcaggctgac gatcagcgcg caggtcttcg tcacggaagc atttaacgat ctgatagtag
     3661 cggtcaaagc cggacatcat cagcagctgt ttgaacaact gtggggattg cggcagtgcg
     3721 tagaatttac ctttgtgcac acgagaaggc accaggtagt cacgcgcgcc ttccggcgtg
     3781 gctttggtca gcatcggagt ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc
     3841 accaggctgg tgattttagc gcgggttttc aggcgctgag ccatttccgg acgacgcagg
     3901 tcgaggtagc ggtatttcag acgcgcttct tcggtgttga cgtggttaga gtcaagcggc
     3961 agaacatctg cgcggttgat gatagtcagc gaggacgcca gcacttcgat ttcgccggtc
     4021 gccatatcgc ggttaatatt tttttcgtca cgcgcacgta cggtgcccgt gacctgaatg
     4081 cagaactcat tacgcagttc agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa
     4141 aatacctgca cgataccttc gcggtcgcgc atatcgatga agatcaggct accaagatca
     4201 cgacgacggt tgacccaacc acacagagtc acctgctgcc ccacgtggga caaacggagc
     4261 tgtccacaat attctgtacg catgagatat cccttaactt agctgccggc ggatgccccc
     4321 tgctgcgcag gtgaccaagt cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc
     4381 ggctattata ctggaaattc tgccgcaccg taagagcctg gcccgcgctg gaacgcctcg
     4441 ttaccacttt atatcgggcc tgaaatcaga ctctacgcca gtttgctata aaggtgttgc
     4501 ccgaactcat aaaaattaac aaaatttgtc gttccgccat cggctaatcg cattaaggtg
     4561 tgaggcacga ttttgttttt tcaggagtca tcatgcttga acttaatgct aaaaccactg
     4621 cgctggtggt gatcgattta caagaaggca tcttaccttt tgccggtggt ccacatactg
     4681 ccgatgaggt agttaatcgc gccgggaagc tggcggcgaa atttcgcgcc agcggtcagc
     4741 ccgtgtttct ggtgcgcgtt ggctggtctg ccgattacgc cgaagcatta aaacagccgg
     4801 ttgatgcccc ttcccccgct aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat
     4861 taggtgcaac tgacagtgat atcgaaataa tcaaacgtca gtggggtgcg ttttacggta
     4921 cggatctgga gttgcaatta caccgccgag gtatcgatac aatagtgtta tgtgggatct
     4981 cgaccaatat cggtgttgaa tccaccgccc gcaatgcctg ggaacttggc tttaatctgg
     5041 tgattgccga agacgcctgt agtgccgcca gcgccgagca acacaataac agcattaatc
     5101 atatctaccc gcgcatcgcc cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat
     5161 ttacatcggt ctaccgcaat ggtcgcatcc taaatgggtg cggttgggga tcaccagcct
     5221 tgaagagtat gcccgccact ttaactgcgt ggagggcaac accacgcttt acgccctgcc
     5281 gaaacccgag gttgtcctgc gctggcgtga gcagaccaca gatgacttcc gcttctgttt
     5341 taagtttccg gcgaccattt cgcatcaggc agcattacgg cattgcgatg atttagtgac
     5401 tgaatttttg acccgcatgt caccgttggc tccgcgcatt gggcaatact ggctgcaact
     5461 gcctgccaca ttcggcccac gggagctgcc tgcgctttgg cattttctcg attctctccc
     5521 tggcgaattt aattatggtg tggaagtccg ccatccacag tttttcgcca aaggggaaga
     5581 ggaacaaacg cttaatcgcg gtttacatca gcgcggcgtt aatcgggtga ttttagacag
     5641 ccgcccggtt catgcagcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa
     5701 acctaaagtt ccggtacatg ctgtactgac ggcgacaaat ccactgatcc gttttatcgg
     5761 tagtgatgat atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca
     5821 gtggcatcag accactacgc cttatctttt tttacatacg ccagacattg cccaggcacc
     5881 ggaactggta cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt
     5941 tccggctatt ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc
     6001 catcggccat tttaaaggga gtttgtatgg taagcgcgct gtatgccgtt ttaagtgcgt
     6061 tgttattaat gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct
     6121 atggtgacgg tggttttagc gaactgcaaa gcgctattcg cattcatggt aacgcggtgg
     6181 aatatattcc catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct
     6241 ggatggtgca tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt
     6301 ttcatcaccg tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt
     6361 tgctgatggt gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt
     6421 agcgcacaat acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg
     6481 ctattttctg cccctatcgc cagactgggc gactggacct ttgatgaacg ggtagctgaa
     6541 gtcttcccgg atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt
     6601 ggtatgttag ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct
     6661 ctgggcgcgg cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt
     6721 gccatcgaca actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa
     6781 gcccctacgc cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca
     6841 tcgatggtgg tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta
     6901 ctggataaaa tttatcaagg gctgaacccc ggcggcgcgc tggtgctttc ggaaaaattc
     6961 agtttcgaag atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt
     7021 gccaacggtt acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg
     7081 ctgaccgatt ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc
     7141 gagctgtggt tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct
     7201 gcatgatcga ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc
     7261 tcgaaacgct gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggttgttta
     7321 agcagtggtc aaatgcggtg gagtttctac ctgaaattaa accgtatcgt cttgatttat
     7381 tgcatagcgt tacggcagaa agcgaagagc cactgagcgc agggcaaatt aaacgcattg
     7441 aaacgctgat gcgcaacctg atgccgtggc gcaaagggcc gttctcactg tatggcgtca
     7501 acatcgatac cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg
     7561 atttaaccgg acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca
     7621 tgattggcgc aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc
     7681 agtttgaagc ggtgcgtaaa ctactgggta atgatcagcg cgcgcatttg ttaccgttag
     7741 gtattgaaca acttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt
     7801 atcatcgtcg ttcaccgctg gagcatctct ggcagttgaa agatcaactg gtcaatgaag
     7861 gtgaactggt gcttgaaacg ctggttattg atggtgatga gaacacggtg ctggtgccgg
     7921 gcgatcgtta cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa
     7981 actggctgaa gaagtgtggt tttgtagata tccgcattgc ggatgtgagc gttaccacca
     8041 cagaagagca gcgacgcacc gaatggatgg ttaccgaatc gctggccgat ttcctcgacc
     8101 cacatgatcc gggtaaaacg gtggaaggtt atccggcacc taaacgcgcg gtgctgattg
     8161 cgcgcaagcc gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc
     8221 tacaaaacct tgctaattca atatattgca gggactatgt aggcctgata agcatagcgc
     8281 atcaggcagc tttatgtttg cataacctca gcgcccgttt ccgggcgctt ttcacgtctt
     8341 acgcctgaac agctggctga tcaaacgccg ttaacttcgg tgcgttaccc gtatattttt
     8401 cgatatacac cagcgcagag ttaccggcac aggcatttgc cagctgcgag ctggggatat
     8461 ccgccgttaa cacgttcgca ctgccgtttt tacacaagcc attttccaga tctggccatg
     8521 caccttcatg cacgcatacc acgccttttt tgatcccgtc agtcaccaca gcgcctgtca
     8581 ggatctgacc tcgtttattc cagacacgga ccagatcgcc attcgcaata ccaaagcgag
     8641 cagcatcttc ggtgtgaata gtgattggtt cacgatcggc aaccgcatat tttttacgca
     8701 gttccgcata gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca
     8761 actgcttctc gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg
     8821 ggcaatcctt atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg
     8881 gcgtacccag cgcatttttc actggatcgg cgcggaaatc accataacga acatactgtt
     8941 cgttcttctc gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca
     9001 ttggcatagt gacgcgttgc gcacgggcac ctttctgagc ggcatcatag aaaaatttca
     9061 gccacgccat ttcatcttta ccttcggtat agatctcttt tccgccaggt ttcagtaatt
     9121 cagcaagatc ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcgacagcct
     9181 gcttcatcgg tacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct
     9241 caaacgatgt ggtgatcggt aatacgatat ctgcatgttt agcagctgcg gtccagtagc
     9301 attcagaaac gacgatcatc tccggtttct gccatgcttt gatcagacga ttagtatcct
     9361 ggtgatgggt aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt
     9421 gttctttacc gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg
     9481 caacaggaat cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg
     9541 ccgcagacat ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat
     9601 gataagagaa accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg
     9661 tcaccagcat ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca
     9721 tcagcattgt gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta
     9781 cgccagtaat ttccgccgcc caggccgcgc tcttcggagt attatcgctc ttacctgtca
     9841 gatattcttc aaactgcgga tacccggtgg tgtatttttc aaggaacact ttatcgtgtt
     9901 tgccttgtgt catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat
     9961 tcggcgcgat ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat
    10021 caatggcaat cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct
    10081 catcggtact gctccaggca atttttaagg tattcagcgg gttcatcccc cacagcacca
    10141 caacctggct gttttccaga atcagcggcc aggaggtctg ctgttcatac acctctacag
    10201 aaccgaccac atgcggcatg atcacctgtg ccgccccggt tgaataatcg ccgctatgcc
    10261 cggaataacc gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca
    10321 cgccagaaga gcgccagccg tatgatccgg caaaaatagc cgatggtccg ttagctttgc
    10381 gaatgcgatc gtgttgctca tgaatcagtt ttaatgcttg ctcccagctc acctgtacat
    10441 aggtatcttc gccacgacct ttcgccggtt gcagtggatt atcgagatag ctttttctca
    10501 ccatcggatg ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg
    10561 agttcggtat ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt
    10621 ctacgttcat cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc
    10681 atgcgggtag cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt
    10741 taataaattc acgtcttgtt aatgtcataa cttcctccct gatcaacgag gatcactgtt
    10801 tctcggtaat atctttggcg ttgtactgga aataccgcgt taaaatgtcc agttcgtttt
    10861 cgctcatgct ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt
    10921 aatggtcggc ggcaataggg gcatgacaac cagcgcaata ggtatcgtca agtttttcag
    10981 cgtattgcca tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag
    11041 acgcctgacg ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg
    11101 ccttgatacc ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga
    11161 gcgtgttttc actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac
    11221 tggcatcaac gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag
    11281 aagttttgaa aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca
    11341 gctcatgcgc cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat
    11401 gacaatcgat acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt
    11461 cactttgcga ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt
    11521 cagttgcttt taattctttc catactgttt cggccatttc ctggcgatga gcttcgaact
    11581 tatcgtcact gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta
    11641 atttagcgaa taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac
    11701 gtatcccttt ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca
    11761 tcgagtggca ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca
    11821 gcaagaaccc gccaccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt
    11881 ttttccct
//
LOCUS       DAZUCE010000153        11770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZUCE010000153 DAZUCE010000000
VERSION     DAZUCE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.153"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            1..236
                     /locus_tag="ACHY03_003525"
                     /pseudo
     CDS             1..236
                     /locus_tag="ACHY03_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(294..725)
                     /gene="tnpA"
                     /locus_tag="ACHY03_003526"
     CDS             complement(294..725)
                     /gene="tnpA"
                     /locus_tag="ACHY03_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element IS609 family
                     transposase"
                     /protein_id="HFS3906714.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPG"
     gene            733..1941
                     /locus_tag="ACHY03_003527"
     CDS             733..1941
                     /locus_tag="ACHY03_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFS3906715.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQACGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            1985..2092
                     /locus_tag="ACHY03_003528"
                     /pseudo
     CDS             1985..2092
                     /locus_tag="ACHY03_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2076..2714)
                     /gene="ytfB"
                     /locus_tag="ACHY03_003529"
     CDS             complement(2076..2714)
                     /gene="ytfB"
                     /locus_tag="ACHY03_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFS3906716.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            2933..3553
                     /gene="fklB"
                     /locus_tag="ACHY03_003530"
     CDS             2933..3553
                     /gene="fklB"
                     /locus_tag="ACHY03_003530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS3906717.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            3862..5274
                     /gene="cycA"
                     /locus_tag="ACHY03_003531"
                     /gene_synonym="dagA"
     CDS             3862..5274
                     /gene="cycA"
                     /locus_tag="ACHY03_003531"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFS3906718.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5319..5981)
                     /gene="ytfE"
                     /locus_tag="ACHY03_003532"
     CDS             complement(5319..5981)
                     /gene="ytfE"
                     /locus_tag="ACHY03_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFS3906719.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6084..7049)
                     /gene="ytfF"
                     /locus_tag="ACHY03_003533"
     CDS             complement(6084..7049)
                     /gene="ytfF"
                     /locus_tag="ACHY03_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFS3906720.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQLLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7157..8017)
                     /gene="qorB"
                     /locus_tag="ACHY03_003534"
     CDS             complement(7157..8017)
                     /gene="qorB"
                     /locus_tag="ACHY03_003534"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFS3906721.1"
                     /translation="MIAITGATGQLGHYVIESLVKTVPASQIVAIARNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELVGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            8106..8486
                     /gene="ytfH"
                     /locus_tag="ACHY03_003535"
     CDS             8106..8486
                     /gene="ytfH"
                     /locus_tag="ACHY03_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFS3906722.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8615..10558)
                     /gene="cpdB"
                     /locus_tag="ACHY03_003536"
     CDS             complement(8615..10558)
                     /gene="cpdB"
                     /locus_tag="ACHY03_003536"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFS3906723.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            10748..11488
                     /gene="cysQ"
                     /locus_tag="ACHY03_003537"
     CDS             10748..11488
                     /gene="cysQ"
                     /locus_tag="ACHY03_003537"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFS3906724.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            11700..>11770
                     /locus_tag="ACHY03_003538"
     CDS             11700..>11770
                     /locus_tag="ACHY03_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906725.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYT"
BASE COUNT         2791 a         2949 c         3064 g         2966 t
ORIGIN      
        1 cttggttttt aaaaattgtg catagtgaaa atcaaagtaa aggtgtgcat tatgcaaaat
       61 cacagcgtct cttagaaatt acccatgcgc acttgcagtt aatggaatcc ttattggatg
      121 aagggaagaa acacaatatt tttaagccag atatcgaccc atttcaggtg aatattaata
      181 ttgctgcgct tgggggatat tatttgatca accagcatac gcttggcctg gtttatccgg
      241 tgcgccgtaa aaccccgtcc ttcagggcgg ggatatacgg cgcggttttc cacctaacca
      301 ggtgtttgct gttgttcaat gtattgacgg atggcggata ttggcgcacc tccgcagcta
      361 ctggcaaaat agccaggact ccacaaaacg cctttgtagt aatacctgac tgcaatatct
      421 ggtcgatctc gtcgcagtaa cctacccgat acgcctttga ggctgtttac cagactggat
      481 atcgccagtt tgggaggata gttaattaac aaatggacgt gatctggttc gccatccatt
      541 tcaaccagtt cagcttcaaa atcagcacat acctttgaaa agtaagtgcg tagtttttct
      601 gtcgcgtcat ggtcaaaaat ctggcgtcgg tatttggtga caaagaccag gtgaacatgc
      661 atcaggaaaa cacaatgcct tccacgccgg atatcggttt cttttttcac agaccaaagt
      721 atcatttggc ctgtgaaacg actacaggca tttaaattcc agttaagacc cggtggtcaa
      781 caggagcgtg aaatgaggcg cttcgcaggc gcatgtcgtt tcgttttcaa tcgtgctctg
      841 gcgcttcaga atgagaatca tgaggccggt aataaataca tcccttacgg gaaaatggct
      901 tcctggctgg ttgagtggaa aaatgccact gaaacgcaat ggcttaaaga tgccccgtca
      961 cagccattgc aacagtcact gaaagacctt gagcgggctt acaaaaactt cttccagaag
     1021 cgggctgctt ttccccgatt caagaagcgg ggacagaatg atgcattccg ctacccgcag
     1081 ggcgttaagc tcgatcagga aaacagccgt atttttctgc cgaaacttgg ctggatgcgc
     1141 taccggaata gccgtcaggt cacgggtgtt gtgaaaaatg tcactgtcag ccagtcctgc
     1201 ggtaagtggt acatcagtat tcagactgaa aatgaagtat caactccggt tcacccttca
     1261 gcattaatgg tcggactgga tgctggcgtg gctaaactcg ccacgctgtc agatggcaca
     1321 gtctttgggc ctgtaaacag ttttcagaaa aaccagaaga cgctggcgag acttcagcga
     1381 cagttaagcc gcaaggtcaa attcagcaac aactggcaga agcagaaacg caaaatacag
     1441 cgactgcatt cctgtatcgc aaatatccgc agggactacc ttcacaaagt cacaacgacc
     1501 gtcagcaaaa accacgcaat gatagtcatt gaggatttga aggtcagcaa catgtcaaag
     1561 tcagcagcgg gtacggtcag ccagccgggg cgcaatgtcc gggcaaaatc aggtttaaac
     1621 cgttcgatac tggatcaggg ctggtatgaa atgcgccgcc agcttgagta caagcagctc
     1681 tggcgaggcg gtcaggtgct tgctgttccg ccagcgtaca caagccagcg ttgcgcgtgc
     1741 tgtggtcata cagcgaaaga aaatcgcctg tcacaaagta aattcagatg ccaggcatgt
     1801 ggatatacag cgaacgctga tgtaaatggc gctcgtaaca ttttagcggc ggggcacgcc
     1861 gttcttgcct gtggagagat ggtgcagtca ggccgctcgt tgaagcagga acccaccgaa
     1921 atgattcagg cgacagcctg aacgtagcag ggatccacgt ccttcagggc gtggaggatg
     1981 tcaacacatc agtatgattt caccccaggc actggaagcc agacgtaagg tcatcaaaga
     2041 gacaatcctt agctggcttc tggttgaccc ttcatctacc gcgcgcgaat aaaactgcca
     2101 tccggctggc gggtaaacag gacctgttga ttattccccg tatcaatggt taagcccgtc
     2161 accacgccgc tggcgttctg gcggattttc accatctgcc cgttttgcaa attactcagc
     2221 ggcttacccg cgccttctac ctgcgccatt gcatagacat cggtagcggg cagtccgtgg
     2281 tcgcggaaca gctgcgccat cgttttaccg ggttcgacac ggtaagagcg ccattgatta
     2341 tcaattccgc tatctggctg gaatggttgc gtttgggtag tttgcggctg ttcttctggc
     2401 tggccttcct gtataggttc tggagcgacc ggagccacct gatcaggatc attttgtggg
     2461 gtgactaatt gcgcccgcag ctgttcttct gtcggcggtt gtgattgcga ctgaatatct
     2521 aactgcgctt cacgcgtcac caccggcgcg ttgggcgtat catcagatgg aagcagaaat
     2581 ccgaccacca atacaatggc ggcaataatg atcccccgac gatgcatcgg cggcagcggg
     2641 tccatgaagc gaaaattgtc cggcgcgtgc cagactttct ccagggttgg ttttagttca
     2701 aagcgcccgg gcatggtttc ctcctgctcc gcgtcttgtt cctcaatcat agcctatgaa
     2761 taagctaacg ctatgatgtc cgtggtaaac ccgcctttat tatattcata cgggatattg
     2821 ctattgtttc tttttccccg ggatttgtca tcattcctgc gacaaagttt tacccaaaga
     2881 agtgtggctg atatgctgcc cgctactttt ataccctaag aaaggaaata cgatgaccac
     2941 cccaactttt gacaccatcg aagcgcaagc aagctacggc attggtttgc aggtagggca
     3001 acaactgagt gaatctggcc tggaagggct gctgccagaa gcactggttg caggtattgc
     3061 cgatgcgctg gaaggcaaac atccggctgt tccggttgat gtggtgcatc gcgcgctgcg
     3121 tgaaatccac gaacgcgccg atgccgttcg tcgtcagcgt ttccaggcga tggctgcaga
     3181 aggtgtgaaa tacctggaag aaaacgccaa aaaagaaggt gtgaatagca ccgaatctgg
     3241 cctgcaattc cgcgtgatca accagggtga aggcgcaatt ccggcacgta ccgaccgcgt
     3301 tcgtgttcat tacaccggta aactgatcga cggcaccgtg tttgacagct ccgttgctcg
     3361 tggtgaaccc gctgaattcc cggttaatgg cgtgatccct ggctggattg aagcactgac
     3421 tctgatgccg gtaggttcta aatgggaact gactatcccg caggaactgg catatggcga
     3481 gcgcggcgca ggcgcatcca tccctccgtt cagcaccctg gtgtttgaag tcgaactgct
     3541 ggaaatcctc taagcagcgc attctgttcc cctcgaacca gaggggagca ggcattcagc
     3601 aataaaccct tcagtttgcc aaacggcgct attttgtgtt gcaaagaccc cgtaagcgtg
     3661 tatttttgtg agctgtttcg cgttatcacc gtgatatgac actcatttta aacataaaat
     3721 taacattaga tctaaatctt agtattcatc ccgcgtattg ttacctaata tcgatgagtc
     3781 ccgatacaga ttcgtcgtat catagactga ctaaaggccg tagagcctga acaacacaga
     3841 caggtacagg aagaaaaaaa catggtagat caggtaaaag tcgttgccga tgatcaggct
     3901 ccggctgaac agtcgctacg gcgcaatctc acaaaccgac atattcagct tattgccatt
     3961 ggcggtgcca ttggtacggg gttgtttatg gggtctggca aaacgattag ccttgccggg
     4021 ccgtcgatca ttttcgttta tatgatcatc ggttttatgc tctttttcgt gatgcgggca
     4081 atgggggaat tgctgctttc gaatctggaa tacaaatctt ttagtgactt cgcttccgat
     4141 ttactcgggc cgtgggcagg atatttcacc ggctggactt actggttctg ctgggttgta
     4201 accggtatgg cagacgtggt ggcgatcacg gcttatgctc agttctggtt ccccgatctc
     4261 tccgactggg tcgcctcgct ggcggtgata gtgctgctgc tgacgctcaa tctcgccacc
     4321 gtgaaaatgt tcggtgagat ggagttctgg tttgcgatga tcaaaatcgt cgccattgtg
     4381 tcgctgattg tcgtcggcct ggtcatggtg gcgatgcact ttcagtcacc gactggtgtg
     4441 gaagcgtcat tcgcgcattt gtggaatgac ggcggctggt tcccgaaagg tttaagtggc
     4501 ttctttgccg gattccagat agcggttttc gctttcgtgg ggattgagct ggtaggtaca
     4561 acagctgcgg aaaccaaaga tccagagaaa tcactgccac gcgcgattaa ctccattccg
     4621 atccgtatca ttatgttcta cgtcttcgcg ctgattgtga ttatgtccgt gacgccgtgg
     4681 agttcggtag tcccggagaa aagcccgttt gttgaactgt tcgtgttggt agggctgcct
     4741 gctgccgcaa gcgtgatcaa ctttgtggtg ctgacctctg cggcgtcttc cgctaacagc
     4801 ggcgtcttct ctaccagccg tatgctgttt ggtctggcgc aggaaggtgt ggcaccgaaa
     4861 gcgttcgcta aactttctaa gcgcgcagta cccgcgaaag ggctgacgtt ctcgtgtatc
     4921 tgtctgctcg gcggcgtggt gatgttgtat gtgaatccta gtgtgattgg cgcgttcacg
     4981 atgattacaa ccgtttccgc gattctgttt atgttcgtct ggacgattat cctttgctcg
     5041 taccttgtgt atcgcaaaca gcgtcctcat ctacatgaga agtcgatcta caagatgccg
     5101 ctcggcaagc tgatgtgctg ggtatgtatg gcgttctttg tgttcgtggt cgtgttgctg
     5161 acactggaag atgacactcg ccaggcgctg ctggttaccc cgctgtggtt tatcgcgctg
     5221 gggttgggct ggctgtttat tggtaagaag cgggctgctg aactgcggaa ataaccgcat
     5281 tatcatgctg gatggcgcaa tgccatccag cttttagatc actcacccgc cagcgcgcgt
     5341 gggaacagta cattgttttc cagactgatg tgatccatca ggtcatcaat cagttcatta
     5401 atgccgttat acatcgcttt ccaggtggtg caggcttctg gcggcggtgt gacgttattg
     5461 gtggtgtgtt taatcacttc cagcagttcg cccgcttcat cgtgctcgct ttccattacg
     5521 ctgattggcc ccattgcctg gctgcccatg ccttgtttga tcatcgggaa gaggatctgc
     5581 tcttctttca tcatgtggct ggaaagctct tcatgcagca tggtcaggta ttttgtcagc
     5641 ccttttggca cgctcggttt gtcggcgtga acgcgctcga ctttagtcgc ttgcagaatc
     5701 agctccggca gttgctcgcg gtgacgatcg tggtagcgca cgatgatatg gtcgatgatt
     5761 tctgccagcg gggcgctacg ccagtctttc tcaatcggtt gttcagcgag ctttgccagt
     5821 tcagcttcaa tgacctcaac atccagttct ttacgtgccg ccgcgcgcgc cagcgtctgc
     5881 ttaccgccac agcagtaatc catatcatat ttacgaaaca gagctgaagc gcgaggaata
     5941 gagagcgcca gttcacctaa aggttggtcg cgataagcca tagctgatac ctcattctta
     6001 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     6061 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     6121 ggttttgctc tgaccgcgat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     6181 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     6241 aaaacaatca gcggcccgag aatcactgtc ggtaatagct ggctggcgac gttccagcag
     6301 agtgcgccaa cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc
     6361 ggacgggggc caaagggtag ggagaagtcc ggcgtttgcg tattcagcca gtaacaagcg
     6421 acgagatagc cgataagtga aaccggcagt gtgaccagcg cctgcgctgt tgcccacatc
     6481 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     6541 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     6601 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     6661 atcagtgctg gggcgagttt tccccacgcg agtttgccgt cgcgctggct ataaagcaga
     6721 ttggcaaaga caggaatgac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg
     6781 ccagtacgtt gaatggcact ggcaaggcag aagtaataga tgaggttgcc catcatagtg
     6841 agcatcaagg cggtaagcca gtcccgacgc gccaactgac gcagacgcac gcgtcccagc
     6901 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcagcatc
     6961 gccgggtatt ccggcacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     7021 aacagggcgt acagcacgcc gctaatcatt actggcatcc attgatctgt cagaagaaag
     7081 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     7141 atgccacttt aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg
     7201 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gtcatcaaat agaccgcctt
     7261 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     7321 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     7381 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccaccagtt
     7441 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     7501 ctgcccgcgt tgctgaggca attttgccat cgcccgcggc accgataaat acgccgtgct
     7561 ccagtgctgc cggtgcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     7621 tgtaaacgat gccagaatca gccagcattt tctccgtctc gatgtgctca tcggcgaggc
     7681 cgagcggaga tttatccgcg tgaagcagac tggtataagc gataaatttc acgcccgccg
     7741 ccttcgccgc gttaatcaca ttgcgatgct gcggggcacg ctggcccact tcgctggaag
     7801 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     7861 agtcagcctg acgcacggta atgccttgtg ctgccagggc ctgggctttt gccgggttac
     7921 gtgcgatagc cactatttgg ctggcaggaa cggttttcac cagggactca ataacatagt
     7981 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     8041 tgtgtttaaa tacgctaaca cctaaactta cttttagtaa gtacgcacaa aaaggtaagt
     8101 atggaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     8161 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     8221 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatc ggcggggtga
     8281 gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca ggatggtttt cttaaccgta
     8341 tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct cacgccactg ggcgagcagg
     8401 tgagtgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttgg
     8461 cggtgcggga tgaacgtgcg gcataactgc cggctgcgct gcgcttatcc ggtcgacggt
     8521 tcatggcttg tagggcggat aagatgcgcc agcatcgcat ccgccaataa atgccggatg
     8581 cggttgatga tttataggcc gaaaaagaag tgttttactt actcaaatcc acctgataaa
     8641 tcgcaaaccc gatatcatcg gtcgcgactt tattcatcgg atactgccct ttctctttaa
     8701 taaacgctgc ggctttatct gacggagagg tttcgaaacg gatatccagt ttcttatcgc
     8761 cagctatcgg tgctaaacgc cagttgttat ctgccgccgg gtgaatttcc cccgcacgtt
     8821 tcgactcatc agcaatccac gctgccagca ccgagcggtt ctcatccggt gaagcaaaag
     8881 cgatatggct gtcgcccgta ccggcaaatt tgccgccgta agcgcgatag ttattggtgg
     8941 caacgaggaa catagcgttc ggatcaatcg gcttgccatt aaaggtcagg ttcttaatcc
     9001 tttccgcatt ggcattaatc atctggcact cgccgtcata acgggcgggc tgggtaacat
     9061 caatctgata attcacacca tcaataacat caaagttata ggtgcggaaa ccgtcccagt
     9121 tgatgagtga ctgtggtttc gtgctgttgg gatcaatctg gttaaactgt ccggcggagc
     9181 actccagcca ctctttcacc tctttaccgc tggctttcac cacaatcagc gtattgggat
     9241 agagataaag atcggcggca ttacggaagg tcaactggcc tttttccacc tccacatagc
     9301 ttgccgggtc atttttgcga ccaccaactt taaacggtgc ggcagctgaa agcaccggca
     9361 gttttgccag atccggatcg ccctgaatgt aatgttcgac atacgctttt tgcgcgttgt
     9421 tcactacttg cacggtcgga tcgtcctgca ccagcgccag atagctatac atattgtcgg
     9481 cagatttacc gattggcttg ctgacgaact ggcgtgtagc atcgtgatcg gctttgagtg
     9541 tttctaccag cttgctgtct tccgccgcga gggatttttt attggcgatg tcgtaaatcg
     9601 gccgtgcttc cgctttcgcc tgcgtcacct gccatttacc gctgtcatta ctgagttgta
     9661 agtcgaccac cccaagatga tcgccccaca tgcctggcat taccgccgga acaccattca
     9721 gcgtgccttt ggcgatatca gccccttcga tatcagcaaa atctttaccc gggaaaacgg
     9781 cgtgagcatg gccaaacata atggcgttaa cgcccggaat ttcactgagg taataaactg
     9841 agttttccgc cattacttta tacggatcgg cagacagccc ggaatgcgcc agaacgacaa
     9901 cgacatcggc acctttctcg cgcatttcag gcacgtattt gcgcacggtt tcggtaatat
     9961 cattcaccgt cacttttccg gataaattag ctttatccca gcccatgatt tgcggcggca
    10021 cgacgccaat atagccaatc ttcagcgtct gtttttttcc gtctttatcg accacttcgg
    10081 tgtctttaat taaatacggt gtaaacattg gctgtttggt tctggcgtca atgacgttgg
    10141 catttacata agggaatttc gctcctgcca gcgcattttt caggtaatcc agaccgtagt
    10201 taaactcgtg gttgccaagc gttccgacgg tatagtccag cgtatttaat gccttataga
    10261 ccgggtgaat atcacctgct tttaatcctt tcgccgacat gtaatcggcc agcggactcc
    10321 cctgaatcaa atcgccgtta tcaaccagta cgctgttttt cacttcattg cgggcatcgt
    10381 taatcaggct tgccgtacgt accagtccga atttttccgt ggcggtgtct ttgtaataat
    10441 cgaaatccat catgttgcta tgcagatcag tggtttccat gatacgcaga tcgaccgtcg
    10501 ctgcattcac actggcggca atcagcgtgg ccaggagcgt tgcgctaaac ttaatcatca
    10561 gggacatcct tttatcatcg ggaatacgaa agaaaaggga gaataaacgc cttacttata
    10621 gaacagtgaa gaatgccaca attttacgct ttgaaaatga tgacactatc acagttggcg
    10681 cattcattaa cgatagggta taagtaaaac aataagttaa caccgctcac agagacgagg
    10741 tggagaaatg ttagatcaag tatgccagct tgcacggaat gcaggcgatg ccattatgca
    10801 ggtctacgac gggacgaaac cgatggacgt cgtcagcaaa gcggacaatt ccccggtaac
    10861 ggcagcggat attgccgcac acaccgttat catggacggt ttacgtacgc tgacaccgga
    10921 tattccggtc ctttctgaag aagatcctcc cggttgggaa gtccgtcagc actggcagcg
    10981 ttactggctg gtagacccac tggatggtac taaagagttt attaaacgta atggcgaatt
    11041 caccgttaac attgcgctca ttgaccatgg caaaccgatt ttaggcgtgg tgtatgcgcc
    11101 ggtaatgaac gtaatgtaca gcgcggcaga aggcaaggcc tggaaagaag agtgcggtgt
    11161 gcgcaagcag attcaggtcc gcgatgcgcg cccgccactg gtggtgatca gccgttccca
    11221 tgcagatgcg gagctgaaag agtatctgca acagcttggc gaacatcaga ccacgtccat
    11281 cggctcttcg ctgaaattct gcctggtggc ggaagggcag gcgcagctgt acccgcgctt
    11341 cggaccaacg aatatttggg acaccgccgc tggacatgct gtggctgcag ctgccggagc
    11401 gcacgttcac gactggcagg gtaaaccgct ggattacact ccgcgcgagt cgttcctgaa
    11461 tccggggttc agagtgtcta tttactaaat tcagatggca gaaacagtgt atttcctgat
    11521 tctgccatcc tgatttctcc caacctaaaa agttataaat aaaaagagat tgtatttaaa
    11581 gtgcaaaaat tcaattgcta ataagttaca ttttaataat gagcgttttt tgatagttta
    11641 cttctatagt gagatattta atggcgacat aaagtaacca aataaaataa ggttgtcata
    11701 tgttacccag gatcagacac aataatttta ttggtgcggt ggagttattt gtaaagtctt
    11761 cgtatacaaa
//
LOCUS       DAZUCE010000154        11764 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZUCE010000154 DAZUCE010000000
VERSION     DAZUCE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.154"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            372..2795
                     /gene="pgaA"
                     /locus_tag="ACHY03_003539"
     CDS             372..2795
                     /gene="pgaA"
                     /locus_tag="ACHY03_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFS3906726.1"
                     /translation="MYSSSRKRCPQTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGQYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQIDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETITPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTSRELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVLRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKSGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2804..4822
                     /gene="pgaB"
                     /locus_tag="ACHY03_003540"
     CDS             2804..4822
                     /gene="pgaB"
                     /locus_tag="ACHY03_003540"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFS3906727.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDEMVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     AGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGLLFHDDALLSDYEDASAPAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVTQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLINQIKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDVIRPEFSTAWYPKND"
     gene            4815..6140
                     /gene="pgaC"
                     /locus_tag="ACHY03_003541"
     CDS             4815..6140
                     /gene="pgaC"
                     /locus_tag="ACHY03_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFS3906728.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYVVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6142..6555
                     /gene="pgaD"
                     /locus_tag="ACHY03_003542"
     CDS             6142..6555
                     /gene="pgaD"
                     /locus_tag="ACHY03_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFS3906729.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6605..7393)
                     /gene="phoH"
                     /locus_tag="ACHY03_003543"
     CDS             complement(6605..7393)
                     /gene="phoH"
                     /locus_tag="ACHY03_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFS3906730.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVHSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8014..9285)
                     /gene="efeB"
                     /locus_tag="ACHY03_003544"
     CDS             complement(8014..9285)
                     /gene="efeB"
                     /locus_tag="ACHY03_003544"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFS3906731.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGTAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYSSDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDVNDYLGSALLRV"
     gene            complement(9291..10418)
                     /gene="efeO"
                     /locus_tag="ACHY03_003545"
     CDS             complement(9291..10418)
                     /gene="efeO"
                     /locus_tag="ACHY03_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFS3906732.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10476..11306)
                     /gene="efeU"
                     /locus_tag="ACHY03_003546"
     CDS             complement(10476..11306)
                     /gene="efeU"
                     /locus_tag="ACHY03_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFS3906733.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSEGLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPLRAGATASRSA
                     "
BASE COUNT         3105 a         2865 c         2746 g         3048 t
ORIGIN      
        1 ttcatagata tattaggaat aacaattgaa tccgtgagta cagtaacgct gcatttcatc
       61 aggactttcg aaaaatccga aatcatgcat cggaatttac tgatttaatt attttactcc
      121 taacttattt tgaaaaaggc attgggattt atgccgtatt cctgaagatc ctcatcattg
      181 gaatggattt tcgggcgaga aaaggatttt atatggacac tctgctcatc atttcttctt
      241 ctcatcatca acaattcacg tctctcttcc gcgtttaata acggattatg aggtgcaaaa
      301 aatatctttc ttttcagtta cctgtcatta gatacagaga gagattttgg caatacatgg
      361 agtaatacag gatgtattca agtagcagaa aaaggtgccc gcaaaccaaa tgggctttga
      421 aaattcttac tgccgcattt ttagcagcga gtcccgcggc gaagagtgcc gttaataacg
      481 cctatgatgc attgattatt gaagctcgca agggaaatac tcagccagct ttgttatggt
      541 ttgcacaaaa atcagcactc agtaataacc aaattgctga ctggttacag attgccttat
      601 gggccgggca agataaacag gttattaccg tttacaaccg ctaccgttat cagcaattac
      661 cagcgcgtgg ttatgcagct gtcgccgtcg cttatcgtaa cctgcaacaa tggcaaaact
      721 cgcttacact gtggcaaaag gcgctctctc tggagccgca aaataaggat tatcaacggg
      781 gacaaatttt aaccctggca gatgcgggtc aatatgatac tgcgctggtt aaacttaagc
      841 agcttaactc tggagcaccg gacaaagcca atttactcgc agaagcctat atctataaac
      901 tggcagggcg tcatcaggat gaattacggg cgatgacaga gtcattacct gaaaatgcat
      961 ctacgcaaca atatcccaca gaatacgtgc aggcattacg taataatcaa cttgctgccg
     1021 cgattgacga tgccaattta acgccagata ttcgcgctga tattcatgcc gaactggtca
     1081 gactgtcgtt tatgcctacg cgcagtgaaa gtgaacgtta tgccattgcc gatcgcgccc
     1141 tcgcccaata cgctgcatta gaaattctgt ggcacgataa cccagaccgc actgcccagt
     1201 accagcgtat tcagattgat catcttggcg cgttattaac tcgcgatcgt tataaagacg
     1261 ttatttctca ctatcagcgt ttaaaaaaga cgggacaaat tattccgccc tgggcgcaat
     1321 attgggttgc gtcggcttat ctcaaagatc atcagccgaa aaaagcacag tcaataatga
     1381 ccgagctctt ttatcacaag gagaccatta ccccggattt atccgatgaa gaacttgcgg
     1441 atctctttta cagccacctg gagagtgaaa attatcccgg cgcgctaact gtcacccaac
     1501 ataccattaa tacttcgccg cctttccttc ggctaatggg cacgcctacg agcatcccga
     1561 atgatacctg gttacagggg cattcgtttc tctcaaccgt agcaaaatat agtaatgatc
     1621 ttcctcaggc tgaaatgaca tccagagagc ttgcttataa cgcaccagga aatcagggac
     1681 tgcgcattga ttacgcgagt gtgttacaag cccgcggttg gcctcgtgcg gcagaaaatg
     1741 aattaaaaaa agcagaagtg atcgagccac gtaatattaa tctggaggtt gaacaagcct
     1801 ggacagcatt aacgttacaa gaatggcagc aggcagctgt cttaacgcac gatgttgtcg
     1861 aacgtgaacc gcaagatccc ggcgttctac gtttaaaacg tgcggttgat gtacataatc
     1921 ttgcagagct tcgtatcgct ggctcaacag gaattgatgc cgaaggcccg gatagtggta
     1981 aacatgatgt cgacttaacc accatcgttt attcaccacc gctaaatgat aactggcgcg
     2041 gttttgctgg attcggttat gccgatggac aatttagcga aggaaaaggg attgttcgcg
     2101 actggcttgc gggtgttgag tggcggtcac gtaatatctg gctcgaggcg gagtacgctg
     2161 aacgcttttt caatcatgag cataaatccg gcgcgcgcct ttctggctgg tatgatttta
     2221 atgataattg gcgtattggt tcgcaactgg aacgcctctc tcaccgcgtt ccattacggg
     2281 caatgaaaaa tggtgttaca ggcaacagtg ctcaggctta tgttcgctgg tatcaaaatg
     2341 agcggcgtaa gtacggtgtc tcctgggctt tcactgattt ttccgacagt aaccagcgtc
     2401 atgaagtctc aattgagggt caggaacgca tctggtcttc accatatttg attgtcgatt
     2461 tcctacccag tctgtattac gaacaaaata cagaacacga taccccatac tacaacccta
     2521 taaaaacgtt cgatattgtt ccggcatttg aggcaagcca tttgttatgg cgtagctatg
     2581 aaaatagctg ggagcaaata ttcagcgcag gtgttggtgc ctcctggcaa aaacattatg
     2641 gcacggatgt cgtcacccaa ctcggctacg ggcaacgcat tagctggaat gacgtgattg
     2701 atgctggcgc aacgctacgc tgggaaaaac gaccttatga cggtgacaga gaacacaact
     2761 tatacgttga attcgatatg acattcagat tttaaggata aatatgttac gtaatggaaa
     2821 taaatatctc ctgatgctgg tgagtataat tatgctcacc gcgtgcatta gccagtcaag
     2881 aacatcattt ataccgccac aggatcgcga atctttactc gccgagcaac cgtggccgca
     2941 taatggtttt gtagcgattt catggcataa cgttgaagac gaagctgccg accagcgttt
     3001 tatgtcagtg cggacatcag cactgcgtga acaatttgcc tggctgcgcg agaacggtta
     3061 tcaaccggtc agtattgctc aaattcgtga agcacatcga ggaggaaaac cgctaccgga
     3121 aaaagctgta gtgctgactt ttgatgacgg ctaccagagt ttttataccc gcgtcttccc
     3181 aattcttcag gccttccagt ggcctgctgt atgggccccc gtcggcagtt gggtcgatac
     3241 gccagcggat aaacaagtaa aatttggcga tgagatggtc gatcgagaat attttgccac
     3301 gtggcaacaa gtgcgagaag tcgcgcgttc ccggctcgtt gagctcgctt ctcatacatg
     3361 gaattctcac tacggtattc aggctaatgc gaccggcagc ttattgcctg tatatgtaaa
     3421 tcgtgcatat tttactgacc acgcacggta tgaaaccgca gcagaatacc gggaaagaat
     3481 tcgtctggat gctgtaaaaa tgacggaata cctgcgtaca aaggctaagg taaatccaca
     3541 cgtttttgtt tggccttatg gcgaagcgaa tggcatagcg atagaggaat taaaaaaact
     3601 cggttatgac atgttcttca cccttgaatc aggtttggca aatgcgtcgc aattggattc
     3661 cattccgcga gtattaatcg ccaataatcc ctcattaaaa gagtttgccc agaaaattat
     3721 taccgtacag gaaaaatcac cacaacggat aatgcatatc gatcttgatt acgtttatga
     3781 cgaaaaccgc cagcaaatgg atcgcaatat tgatgtgcta attcagcggg tgaaagatat
     3841 gcaaatatca accgtttatt tgcaggcatt tgctgatccc gatggtgatg ggctggtcaa
     3901 agaggtctgg tttccaaatc gtttgctacc aatgaaagca gatattttta gtcgggttgc
     3961 ctggcaatta cgtacccgcg caggtgtaaa catctatgcg tggatgccgg tattaagctg
     4021 ggatttagat cccacattaa cgcgagtaaa atacttacca actggggaga aaaaagcaca
     4081 aattcatcct gaacaatatc gccgtctctc gccttttgat gacagggtca gagcacaagt
     4141 tggcatgtta tatgaagatc ttgccggaca tgctgctttt gatggcctat tgttccacga
     4201 tgatgctttg ctttcagatt atgaagatgc cagtgcaccg gctatcgcgg cttatcagca
     4261 agcaggcttt agcgggagtc tgagcgaaat tcgacaaaac ccggagcaat ttaaacagtg
     4321 gacccgcttt aaaagtcgtg cgttaactga cttcacttta gaacttagtg cgcgcgtaaa
     4381 agccattcgt ggtccacatg ttaaaactgc acgaaatatt tttgcacttc cggtaacaca
     4441 acctgaaagt gaagcctggt ttgcacagaa ttatgctgat ttcctaaaaa gctatgactg
     4501 gactgctatt atggctatgc cttatatgga aggtgtcgca gaaaaatcgg ctgatcaatg
     4561 gttaatacaa ctgatcaatc agattaaaag catccctcag gctaaagaca aatctatttt
     4621 agaattacag gcacaaaact ggcagaaaaa tggtcagcat caggctattt cttcgcaaca
     4681 actcgctcac tggatgagcc tattacaact gaatggagtg aaaaattatg gttattatcc
     4741 cgacaatttt ctgcataacc aacctgaaat agacgttatt cgtcctgaat tttcaacagc
     4801 ctggtatccg aaaaatgatt aatcgcatcg tatcgttttt tatattatgt ctggtgttat
     4861 gcatccccct atgcgtagcg tactttcact ctggtgaact gatgatgagg ttcgttttct
     4921 tctggccgtt ttttatgtcc attatgtgga ttgttggcgg cgtctatttc tgggtctatc
     4981 gtgaacgcca ctggccgtgg ggagaaaacg caccagcccc ccagttgaaa gataacccgt
     5041 ctatctccat tatcattccc tgttttaatg aggagaaaaa cgttgaggaa accatacacg
     5101 ccgctttagc acagcgttat gagaacattg aagttattgc cgtaaatgac ggttcaacag
     5161 ataaaacccg tgccatcctg gatcgcatgg ctgcacaaat tccccatttg cgggtcattc
     5221 atctggcgca aaaccagggg aaagccattg cgcttaaaac cggagctgcc gcggcgaaaa
     5281 gtgaatatct ggtgtgcatt gatggcgacg cgttattaga ccgcgatgcg gcggcatata
     5341 ttgtggaacc gatgttgtac aacccgcgtg tgggtgccgt aaccggtaat cctcgtattc
     5401 gcacacgttc taccctggtg ggtaaaattc aggttggcga gtattcctca attattggtt
     5461 tgatcaagcg aacccagcgt atctatggaa acgtatttac cgtttccggt gttattgccg
     5521 catttcgtcg cagcgccctg gcagaagtgg gttactggag tgacgatatg atcaccgaag
     5581 atattgatat tagctggaag ctgcagttga atcagtggac gattttttac gagcctcggg
     5641 cgctgtgctg gatattaatg cctgaaacgt taaaagggct gtggaaacag cgcctgcgct
     5701 gggctcaggg cggtgcagaa gtattcctca aaaatatgac aaggctatgg cgcaaagaaa
     5761 actttcgaat gtggccactg ttttttgaat actgcctgac gacaatatgg gcctttacct
     5821 gcctggtcgg tttcattatt tacgtagtcc aacttgccgg tgtaccgtta aatattgaat
     5881 tgacacatat cgctgcgaca catactgccg gaatattatt gtgtacgtta tgtttactgc
     5941 aatttattgt cagcctgatg atcgagaatc gctatgagca taatctgact tcatcgcttt
     6001 tctggattat ttggttcccg gttattttct ggatgctgag cctggcaacg acattggtat
     6061 catttacacg agtcatgttg atgcctaaaa agcaacgcgc ccgttgggta agtcccgatc
     6121 gcgggattct gagaggttaa tatgaacaat ttaattatta cgacccgaca atcgccagta
     6181 cgtttactgg ttgattatgt tgccacaacc atcttgtgga cattatttgc tttgttcata
     6241 ttcttattcg ccatggatct gctgacgggt tattactggc aaagcgaggc cagaagccga
     6301 cttcagttct attttttgct ggcagtggcg aatgccgtcg tgttaattgt ctgggcgctg
     6361 tacaataagc tgcgttttca aaaacagcag catcatgcag cctaccaata tacgccgcaa
     6421 gaatatgcag agagcttagc aatacctgat gagctctatc agcaactaca aaaaagccac
     6481 aggatgagcg tacacttcac cagccagggg caaataaaaa tggtggtttc agaaaaagcg
     6541 ctagtccggg cataaacacc caaaacaaag cccggttcgc ccgggctctg caccgataat
     6601 acacttaact gtatgcatgt agcgtacgtt ggcaaagtgc cgaacgtacg caatcctctt
     6661 taccgaatcg gacgatcccg accatttcat cttcttcgaa acgttccagt gcgtcactta
     6721 atccggagtg cacgccgcga ggtaaatcgc attgcgtgat atcaccgttg acgataaccg
     6781 tcacgttctc cccgaggcgg gttaaaaaca ttttcatttg cgcggcagtc acattctgcg
     6841 cctcgtcaag aatgacgact gcattttcaa aggtacgtcc acgcatatag gcgaacggcg
     6901 caatttccac cttccctatt tccggtcgca ggcagtactg cataaaggaa gcccctaagc
     6961 gccggaccag cacgtcgtat accgggcgaa aatagggagc aaacttttct gcgatatctc
     7021 caggtaagaa gccaagatct tcatcggctt gcagaactgg acgggtgacg ataatcctgt
     7081 cgacatcctt atgtatcagg gcctctgccg cttttgctgc gctgatccag gtttttccgc
     7141 acccggcttc gcccgtagcg aatatcagct gcttactctc aatagccttc agatagtgca
     7201 attgcgcttc atttcgcgcg aggatgggcg aagtatcgcg actgtcgcgg gccataccaa
     7261 tggcttctac gccgctcatc tgcacaagcg aggtgaccga ttcttcttca cgctgcttat
     7321 ggctgcgcga atcccgtctc agcacacgtt ttgcctcgcg acgagctttg atcactgctt
     7381 tttgtcttcc catggagagc accttgagtt gtttgtattc atcacacgcg ccgttggcag
     7441 cgcgattatg cgcacgaaca tcagagggtt ggcttccttg taagccatag tttgcttttg
     7501 gataaaatgc cgaaaaacgg ctacgcgcac cgtttacggc gtcggtaaca catgaaaaga
     7561 aaggatgagg ttgaaaatgc aaagtgacga gatgactacc ggaggagaaa actccgcgag
     7621 tggtggcgcg ttgattatct aaaacatgtc cagtacagga cgttaccatc cgcgatctcc
     7681 atagtgactg actatcactg ccgggaactt ccgctgctac ttaataagta caacagatct
     7741 cgcattcatt gcaacaatat attcacttat atttaactat aaaacaccat tttagtgaca
     7801 ttagtttgta ctggaaagat gacagagtga tgacagagat gaaaaaagct gtgtgctttc
     7861 agcaggattc acatcaggca tcagtgcacg atcgccggat gcggcgtgaa cgcctgatcc
     7921 agcctaccaa acccgcaccg attgtgggca tgataagcat cgcaagcgcc gcatcaggca
     7981 tctgcgcaca aatgccttat ccgcctaaaa acattaaacc cgcaataatg cgcttccgag
     8041 ataatcgttc acgtccttca ctcccggcag cgcaaaaaaa taaccgccgc cgatgggttt
     8101 aacatattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     8161 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     8221 gcccagtgaa taactgtagc cacgacgcag catcaggctg gactcactct ccgccgtgcg
     8281 gggattcgcc agccggatat ggctgtccag cgcaatcacc ttcccttccg ggtcgctgga
     8341 ataatccggc acatcgtgct catgctgcat tcccagcggt gcgccggttt gcttatcacg
     8401 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     8461 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     8521 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     8581 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     8641 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     8701 atcgcgcagc gcatggatca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     8761 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     8821 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     8881 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     8941 caggcgcgga tttggcgttt ctggtgctgt tccgccctga gtcagaaaag caaaacgctg
     9001 agtcaacaag cgaaacaacc gttcaagatc ggctttatcg ctggcaagta catcaaacgc
     9061 caccagcatc attgcggcct gttgtggcgt cagtattcct gcctgatgct caccataaaa
     9121 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     9181 tgcatgagcg accggacaac ttcccgccag cgccagtgca ccaatccctt tcagtaaacg
     9241 tcggcgtgac ggttcattca cgccgttttc atcttcatac tgcataacgc ttaatccagt
     9301 cccagcacac cacgaagttg cgccagatct tccgccagcg cagtaatcgg tcctttcagt
     9361 gcattccggt cggcatcggt caatttgtcg taggtttcaa aaccgtcttt agtacggtat
     9421 ttcgccagaa tggtatcgac ctttttaaag ttggcatcga cttttgccaa cagttccggg
     9481 ttggcttttt gcagttgtgg acgcagcaga tcgacaattt tctgcgagcc ttcaacgtta
     9541 gcctggaaat cccacagatc ggtgtggctg tagcgatctt cttcaccgct gattttgctg
     9601 gctgccactt cctcaatcag tccggctgcg ccgccgacca cttttgaagg tgggaaagcc
     9661 agttcactga tgcgtttttg caaatcgacc acatcggtat aaagctgctc agcgtactga
     9721 tccatccctt tggtggtgtt gtcgccaaac aatgcttttt ccagacggtg gaaaccggtg
     9781 aattttggat cagcggcttt ttgctcgtaa tcatcttcac gggcgtcaat gctgccatcc
     9841 agatcggaga acagttcagc aatcggttca atgcgctcat agtgctggcg cgtcggcgca
     9901 tacagtgctt tcgctttttc gatatcgcct gctttaatcg cgtcggtaaa ggctttagtg
     9961 tcggtcacca gctgtgtggt ttccgccatg acatacgctt tatatgcagt aattgcacca
    10021 ccaagactta acagcgcatc actttgcgcc gcatccgccg ttgcctcacc tttgacaatc
    10081 aacttccctt tcgggttagt cagcagaccg caggtcatat cgtattcgcc aggctgtaaa
    10141 ttcgccgtca ttttctggct aaaaccaggg gcgatatttt cccgctcttc caccaccatc
    10201 acgcctttga ggatctccca ctccagcgcc ttctggctat ggttctgaat aatgaactgt
    10261 gttttcccgg cgttaaccgt aatggtcatc ggttcgcact gtttatccgt cacggtcact
    10321 ttgacttgcg gaatatcagc ggcgttagcc ataaaagcag aagagaacag cgcagccacg
    10381 ctcaactgca atgcgttacg gcggaagtta atggtcatga cgagactatc cctttaaagt
    10441 atgttgtaac taagcaagag tttgcgtcgt atttgctacg cggagcgaga cgctgtcgcc
    10501 cctgcgcgta gtggcagggc aaatgccacc agcgccggga tgagataaat aaaccagacg
    10561 gcgacttcgc tgacgctcgg tgcttcctga tagccaaaaa tcccttccat cagcgtgcca
    10621 aacagcgagt gagttgagag cccctcgctc atatcgaagg cgatttcctg aaagtggttc
    10681 cacaatccgg cttcatgaaa tgcgcgaatg gcaccagctg ccagccctgc ggcgacgaag
    10741 agaataaaca ggctggtcca tttaaaaaat gcaccaagat tgaggcgaat accgccccag
    10801 tagagcagga aaccgagcac cacggcagta gcaagaccga gcattgcacc cagcggcggc
    10861 cagatcccga catcttgttg aaatgccgcc agcaggaaaa agaccgactc caggccttcc
    10921 cttgcaacgg caaaaaagac catcatcacc agcgcccagc catgatgatt tccacgctgc
    10981 aatgcgctat cgactgcctg ctccagttgc actttgacgt tgcgcgacac tttgcgcatc
    11041 cagaaaacca tccaggtaag gatcaccacg gcgatcaccg ccacgatacc ttcaaacagt
    11101 tcctgttctt tttgcggaaa ttcgccggtg gtttcgttaa tgaagatacc caagcccagg
    11161 cacaacgcag cggcaagcaa cacgccaatc cacatcacac caatccatcg gcctcgctgg
    11221 gtacgcttaa gatagctggc aatcaaactg acaatcagcg cggcttcaag tccttcgcgc
    11281 aacataatga gaaacggaac aaacatgcca cccaccctta aacgtttttc tgagtcaatc
    11341 tatgcaaaga aaagtaaatc gcagtgatag cgattatcat tacgctaaag aaaaatacaa
    11401 gcggaatgtt tataaaatga tgttggaatg taaaaacgag gacaacaaat ccggaaacaa
    11461 aagcgattat tttaaggtat ttagctgact tttcgcccgg cagatgaagc ttgtacgaat
    11521 gttggtggcg acaaactcgc tgcagcgtgc cccgaagggt tgaattttca gttagtcgta
    11581 tcacaaaaga acgttgtttt tgcttattaa tagatagtga tagggttgtg aatgaaatta
    11641 tacctcgtct cctcatcgaa tttttcgccc acaacctaaa caggagaaaa tgtgtattta
    11701 agtctggaat gagcgatttt tgatagtgcg taattaaccc aagaaattat aatccccctt
    11761 atta
//
LOCUS       DAZUCE010000155        11719 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZUCE010000155 DAZUCE010000000
VERSION     DAZUCE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.155"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            153..3935
                     /locus_tag="ACHY03_003547"
     CDS             153..3935
                     /locus_tag="ACHY03_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906734.1"
                     /translation="MGRKSREKRERKLTEQKVDYDSLIHDMSRYRELFTNGVGEDTYF
                     CKHLEATQNLFRRYKTLDVAVALFTSELWPANTGSPIKHIFAWCVLLELPFESQVGIS
                     IDSYIDFKVFTKELYASWPEFPMLEDYSPEADWGQIKVRLGQDFVPMFYGSCIERMSD
                     FVEAFRITYAHIPEAQAHMDLAIALQAQIIESIPELMLSVNTEAQCADVELATEDFWL
                     TCKSLLQQLGNELANRRKKAGCTFDTRVGTFKAPLKPDAFGNAVMQGMALPFLAVEMD
                     RVWIPMSVRSAPGLIIDHWANKNLNGISPSTHRKLAEFVAERFQRTLIGPLTLYVDGT
                     ACKDLPISCIILGNSGIYLICTCDHTSNDRLSNAAESIYAKVSRGSPIFFRLDDGRFL
                     SLSRVGAEPSVDELHIVIVVTLAGTAFNSINLPSKPTRLFPLADFITIFDSLNDLEEL
                     ESYWKFTDAQEGTLSPFSRGTVDLFASFKDMHGVLVEGAIAPNFIGLDPHWGTSWRFK
                     ILTEFWSRAPKVFPDSSIGWTLSSSTEGVVRLESRHHKAMAYSTSIGSCTVQTLIEIT
                     KELRFEDGRLIDLFAQLLADCSYRCRKMMSDISLFRQPHVLFTCSPEPSSLVKAEESP
                     SSLDEFKHVVTSVIPDSHNPDVFHVQVDTRAVLAGLNLSKDGSFEVRCLLETLEKCHA
                     VLGLQMPDEFAQRFTHIASQAARYHLKVMTRNIDVPDYVYPIIPSPTDYKLARRKLAA
                     EIMGLGFIPGRYELSDAKARIDPSSARMRLHIENRLAAFDRHQLLQAFIEQHDALLVT
                     ERTRIQRARLSLAHEVEYDRLDAIEHARKEFGDKARHYRYLLEKTVNLSSNGVDRVTE
                     DVLRELVGLVDWFMVLTGASDTLHNGIDVGGVEIDDSYIPEVFYSTDFGDREATFAKE
                     YAKSRLGLGTNDKDAVEGESEGLLASEKLKKAFITDLGFELQNMLTVLAVLSQAQRYG
                     FGNELSLSYAAAPDRLARGLADSIDRLDYSEAQKIVAFLTLSEVGVRRLVGRDIDEGD
                     IPYWERNKRIDRYTIKPLVIDGADLRWGAESASRAMYIWMSAVRDGYLPAEFDWPHVK
                     PVIREVKESIEKRLELRTEEIFRRHTPYVQRGIDFFRRFREEKFDDVGDFDVFAYWPE
                     SNLLVTVECKYNQPPFTMKDSRRLRDRIFGKAENDRAGQFSRILDRRKFLETHRSRLL
                     ELLQWPQPESVPLRNIELYVSRDVYYWMVHPPYPVPTQFVRVDTLDTWIKTELNIP"
     gene            3940..8367
                     /locus_tag="ACHY03_003548"
     CDS             3940..8367
                     /locus_tag="ACHY03_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HFS3906735.1"
                     /translation="MSEDIKKTAITAAGYIYQNRQGLKVLCDWLDAPTRYSRVKFECD
                     NENEAPQGLDDIVIERSDGFVDLQQIKFTPDSETHLLSWDWMLEKTGKTERSRSMLRK
                     WFDAFKQLDPVRIGEISLITNRRPDAAIEVCLERDRIDPSKIPEPQRTLVEVELGGVE
                     ECKLFFRQLCIRHSDKGYLALEHEVDARLRLHGTPEGIANLKYVALNWATQKNLPAPD
                     GWIKLAEVGTILRASPPAPLPEDFVVPEGYEVPDEIFHQEFVQSTINATGKAIVLTGP
                     PGRGKSTYLSALCDTLAEKGIPTVRHHYFLSTTERGRDRVNSYIVEQSILAQIQQFHE
                     DVPKTGGGLHTQLEACAAHYKVLGKPFVLILDGLDHVWRINAEDKRPLDDLFCQVIPC
                     PENMVLLVGTQPVNDEQLPADLLAFAPKSEWHTLPAMSESAVLSYLRRTVQEGWLTTG
                     FESEEQIEEQLQSAASALRKKTNGHPLHVIYATSELKHSGRRLSSWDIEQLKGDLSQD
                     ARFYYASLWERLAPSQKDTLRLICAFTFFWPKTAFSEIATKIKAVEPDVDKVEHLLHS
                     SVAGLKVFHESLAVFVRATDDYESRINELMPVIADWLEHQAPTSLRVNWLWTVQAKLG
                     NPNNLITGLTRDWIMSRLKEGYSEALFEILLSEALSAALETANFSDAYRLAHLKARMV
                     DGSQFQMQDSDLARLISFTLTLTSEESVIRESIASRHETDILHVVALGLALLSRGDII
                     QAEICGEEAFHRFRALCQFSNKYNSRAGSDELLFLASAFTQLGVIADTSENLAWLVDK
                     NSPDIWLPRVQMLIQKGNLDDLMLLAASLPDGQKKNIISDACIRAAAVAGVVVTERED
                     FDELVRTPFVAAIEVALIKTSTPSSQPIPVNWISDSYYKHKDNLATLTHHWLFGAVHL
                     NLCMIAEGQTNFEHIKAPMYEDREDITDYLNVLSGIGAQIAQRWWCGEFINFHELYEL
                     LEPVESRRFRQSYFRKSATADFQAALHRIACDIYIGSVLLSGTSAVVLSQSTIDAALQ
                     CAWFDSASFRTQYASGSLIRMADEAAITFIGAQRLLLDADIQNETSVHLQIPLQLCAI
                     AISHQLNSEARELCTQTWELTTGYGHRKDPTLSNTVDAIAYLVDVVPKEACRLLAKIA
                     PQIHHVLDYTDGKGTRHVLAEADRLLAKLKPSALVIKFEEHTCVGDWALAESSMQAYI
                     KQGIIEEWPLDALMRTGLHPEIRDTLQQLVEDGCTTASERLRILEEHNGWDIGCLQRH
                     ENSSSDADNKPYEGDVTVFRPEQLEDLLKVLEPYYKERPKLLRTWYQHWESQKQGRNL
                     LVELDKILFAKNNKWMREALHLSEFAFQTKRRLSGLTAAWKYLVHAQIRNGGWIGYME
                     NVDVTCKRLDLVVQNYPQRCDEFVAVTTYNMFDEPVSSRLAPSEVMVYFYVKQNRIDE
                     AVKFTETMVNCVIEDTRTLPLEPPCWMSKLLEPIAQEVK"
     gene            8364..9812
                     /locus_tag="ACHY03_003549"
     CDS             8364..9812
                     /locus_tag="ACHY03_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HFS3906736.1"
                     /translation="MNDLRILIARLGWPATSARWWTIQELAARLGEPETKAETESLLL
                     QLLRSRKLEAEVVEVLCIFWMAIKEFNYFPTTLLAENILLPSPLSSLLMKDFGLSILL
                     MNTDLELAPEDFIIPDDFDSVQGTDLPRIFHITISKLETFTRCPFIRQMAFEWTKNRT
                     AYPNAPYQGDAGYFIRPLGEGFAPHFSSRTALRVISAYLRTLAVARHIWRIPMELTEQ
                     HSLLALPIHPTLAFLRPYRPEWLSVSTEFKSDSKAIEASLRTFFAQVEAANPGDELIA
                     FSSPISMSMEQCVELSIVRWSQTVSSDKEGADSLTSHIESFWTREQMLSSQAPDPLST
                     TTLITPPTFEQLVNKTCMAWPLAGTLDFGRIGYLQHDLYPSRLFLPTMPGIENSLEVI
                     PHDGRLQVKLKDLVVAELYYWNAGWGPARLGQIQGNCGTALISRGTTYRERNITNTGS
                     LRSFYSWQVRRFHRNGSYGRFSEKITRGVVFV"
     gene            9911..11608
                     /locus_tag="ACHY03_003550"
     CDS             9911..11608
                     /locus_tag="ACHY03_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906737.1"
                     /translation="MSQFKFTELHAISEKPQNDDAAWLIEAERSIKFLKENAQNDEVV
                     IYAIGNSVLIHGALALEKNVTTADRWALQNMRMDVEYCWKIQKSWGGGQGHRIYLEPP
                     LDGVFKGGEALIYRRRFHGVEEGRAPIELSQKLVHSLGLYYLPERNAYCRLDERGDIE
                     DVIRIINKNISPPFNYLDIVTIKRKDLDTFMALSKTCLVLKFDFTRVRWGNFAGWGNI
                     NRYTKDEGSLFYHGGINGEASYCNGVMIIRPQVTVRGLVKAWKDEGNPSKRKYATFKI
                     YDRKNSCNVETSCGPDSLSNYFQENKLPWEVSPAFFRAEVLHRFKADPEKYTLDERSI
                     ACRNAWYLKSYDINEAGQVHVYIGDLAHLPYEEQLYWQAFNEWPKGTISKRAFQTDIV
                     GEWYLSNEPLLALKHKISLMDKNPPTWWKPRGTVLSDAVQYPATDSIKEWGDEILALD
                     QYLVEGFLPKPLSEIAKYEGRIIEPKWGSLRILQEALIAKSATVDEAKMLVQPMQKLH
                     ALRTEVRGHASTEKKKKAILEARTDHENLRAHFTSLVGQCEASFNDILRIFDFKIDD"
BASE COUNT         3244 a         2402 c         2800 g         3273 t
ORIGIN      
        1 atttgtcgga tgtgtttgat agtttgaggc tatgcacttt tgtgagggcg gtacagtaag
       61 tgactcaaga tattcattat gatacgcatt aataatttct atgcttcgat accgtataga
      121 ctgtcaacgt atgcatacag ggaggatatg aagtgggtag gaagtcacgt gaaaagcggg
      181 aaagaaaact aacagagcaa aaggttgatt atgattcttt gatacacgat atgtccagat
      241 atcgtgaatt gtttacgaat ggggtaggtg aagacacata cttttgtaag catctggaag
      301 ccacccagaa tttatttcgc cgatacaaaa ccctcgacgt agcagttgca ttatttactt
      361 ccgaactctg gccagccaat acaggtagcc ccataaagca tatttttgct tggtgtgtcc
      421 ttttagagct gccatttgag tctcaggtag gtatatcgat tgatagctat atagatttta
      481 aagtatttac aaaagaatta tacgcatcat ggcctgaatt cccaatgctt gaggattatt
      541 cccccgaggc tgattggggg caaatcaaag tacggcttgg tcaagacttc gtccctatgt
      601 tctacgggag ttgcattgag agaatgtctg acttcgtaga agcatttcgc attacctatg
      661 ctcatattcc agaagcacaa gcccatatgg atcttgccat tgcgctgcaa gcgcagatca
      721 tcgaatcgat tcctgagttg atgctctctg tcaacaccga ggcccaatgt gctgatgttg
      781 aactagctac tgaagacttc tggttgactt gcaagtcttt gcttcaacaa cttggcaatg
      841 agctagcgaa ccgccgaaag aaagcagggt gtacgttcga tactcgtgtt ggcactttca
      901 aagcaccgct gaaaccggat gcgttcggaa atgcagtgat gcaaggcatg gcgctacctt
      961 tccttgctgt cgagatggat agggtatgga taccaatgtc agtacgcagt gcaccgggtc
     1021 tcattattga ccattgggct aacaaaaatc tgaacgggat tagcccctct acgcacagga
     1081 aacttgcaga gtttgtcgct gagcgattcc aaagaaccct tatagggcct ttgactctgt
     1141 atgttgacgg caccgcctgc aaagacctgc ctatcagttg tattatactg ggtaattctg
     1201 ggatatatct catttgcaca tgtgaccata catctaatga ccgcctgtca aacgcagcgg
     1261 aaagcatata cgccaaggtg agtcgtggat cgccgatttt cttccgtctt gatgatggac
     1321 gtttcctttc tctatctaga gttggtgcag aacctagcgt cgatgagctt cacatcgtta
     1381 ttgtcgttac actcgcgggt actgcgttta attccatcaa tctcccctca aagccaactc
     1441 gtttgtttcc attagcggat ttcatcacga tttttgatag tctcaacgat ctggaagaat
     1501 tagaaagtta ttggaaattt acagatgctc aagaggggac gctcagccca ttttcccgtg
     1561 gaacagtaga cctgtttgca tcattcaaag acatgcatgg tgtgcttgtc gaaggtgcga
     1621 ttgcacctaa cttcattggg ctagaccctc actgggggac ttcttggcgc ttcaagatat
     1681 tgacagaatt ctggtctcgt gcacccaagg tatttccaga cagctctatc ggatggacgt
     1741 tgagttcttc aactgaaggt gtcgttcgac ttgaatcgcg tcatcacaaa gcgatggcat
     1801 actcaacatc gattggttcc tgtaccgttc aaacattaat agaaataacc aaagaattac
     1861 gctttgaaga tggacgtctg atagaccttt ttgcccaact tctggccgac tgtagctatc
     1921 gctgcagaaa aatgatgtct gatatttctc tatttaggca gccgcatgtt ttgtttacgt
     1981 gctcccctga accatctagt ctggttaaag ctgaagagag tccttcttct ttagacgaat
     2041 ttaaacacgt ggtgacttca gtcatcccgg attcccacaa ccccgatgta tttcacgttc
     2101 aagttgatac acgtgcagtg cttgctggat taaatctttc caaggatgga tcattcgagg
     2161 ttcgttgctt actggaaacg ctcgaaaaat gtcatgcagt acttggtttg cagatgcctg
     2221 atgaatttgc tcaacgattt actcatatag cgtcgcaagc ggctcgttat catttaaaag
     2281 tcatgacccg taatattgat gtccccgact atgtataccc tataattcct tcgcctaccg
     2341 attacaagct tgcccgtaga aaactggccg cagaaatcat ggggcttggg tttattcctg
     2401 gcaggtatga actgtcagat gccaaggcca ggattgatcc ttccagtgct cgcatgcgtc
     2461 ttcacattga gaatcgactt gcagcatttg ataggcatca gcttctgcag gcattcatcg
     2521 agcaacacga tgccctcctt gttactgaga ggactagaat ccaacgagcc cgtttgagtc
     2581 ttgctcatga agttgagtat gacagacttg atgcgataga acatgcacgc aaagaattcg
     2641 gtgataaggc ccgccattat cgatatctac tagagaagac cgtcaattta tcttcaaatg
     2701 gtgtagatag agttacagag gatgtactac gagaattagt tggcttggtt gattggttca
     2761 tggtgctgac tggtgccagt gatacccttc acaatggtat agatgtgggt ggtgttgaga
     2821 tcgacgactc atatattcca gaagttttct attcgacaga ttttggtgat cgtgaggcta
     2881 cctttgcaaa ggagtatgcg aagtccaggt tggggcttgg cacaaatgac aaggatgcgg
     2941 tggaaggaga gtcagagggg ttgctggctt cagagaaact caaaaaagct tttatcaccg
     3001 atttggggtt cgagcttcag aatatgctca ctgttctcgc tgtgttatcg caagctcaac
     3061 gatatggatt cgggaacgaa ctttccttgt cttatgctgc agcacctgat cgccttgcgc
     3121 gagggttggc tgatagcatt gataggcttg actattctga ggcccaaaag attgttgctt
     3181 ttctgactct ttcggaagta ggagttcgta ggctcgttgg aagggatatc gatgaaggag
     3241 atattccata ttgggagcgg aataagcgaa tcgatcgata cactatcaag ccgctggtaa
     3301 ttgatggtgc cgatttgcgt tggggggcag agtcggcaag ccgggcaatg tacatttgga
     3361 tgtcggcggt acgagacggt tatttgcctg cggaatttga ttggcctcat gttaagccag
     3421 tcattcgcga ggttaaggag agtattgaaa agcgtctcga gcttcgtaca gaggaaatat
     3481 tccgcaggca tacaccttat gttcagcgag gtattgattt ctttagaaga ttccgggaag
     3541 agaagtttga tgacgttggt gatttcgatg tttttgcata ctggccggaa agcaatttgc
     3601 tcgtgacagt agagtgtaag tacaaccagc cgccatttac aatgaaagat agccgtcgtc
     3661 ttcgtgatcg gatatttggc aaggcagaga atgacagggc aggacaattc agtcgtattt
     3721 tagaccgcag aaaatttctc gaaactcacc gttcaaggtt gcttgaactg ctccagtggc
     3781 cacagccaga gagtgttcct ttaaggaata ttgaactcta tgtaagccgc gatgtgtact
     3841 actggatggt tcatccgcca tatcctgttc caacccagtt tgtacgtgta gacactttgg
     3901 acacttggat caaaaccgaa ctaaacatac cttgatgcta tgagtgaaga tattaagaaa
     3961 accgccatca ctgcagctgg ttatatttat caaaatcgcc agggccttaa ggtcctgtgc
     4021 gattggcttg atgcgccgac gcgctatagc cgcgtgaaat ttgaatgtga taatgaaaac
     4081 gaagcaccac agggactaga tgatatcgtc atcgaacgaa gtgatggctt cgttgacctt
     4141 cagcagatca agttcacccc agattctgag acgcatcttc tatcttggga ttggatgctg
     4201 gagaaaacgg gcaagacaga gcggtcgaga tcgatgctac gcaaatggtt cgatgctttc
     4261 aaacaacttg atccagtgcg tattggcgaa atttcgctca taacgaatcg ccgaccagat
     4321 gctgctattg aggtttgtct ggaacgagac cggattgatc cttctaagat cccagaacct
     4381 cagcgcacct tggtagaagt tgaactcggc ggcgttgagg aatgcaaact tttcttcaga
     4441 caattatgta ttcgacacag cgataagggc tatcttgcat tggagcatga agttgatgct
     4501 cgccttcgac tgcatggtac gccagaggga atcgctaatc taaaatatgt ggctctgaat
     4561 tgggcaacgc aaaaaaatct ccctgcgccg gatggctgga ttaaactggc agaggttgga
     4621 accatcttgc gagcttcacc tccagctcct ttgccagagg attttgttgt tccagaaggt
     4681 tatgaggttc ctgatgaaat attccatcaa gaatttgtcc agagcacaat taatgctacc
     4741 ggaaaggcaa ttgtgctgac tggtccacca ggacgcggaa agagtactta cctgagcgca
     4801 ctgtgcgaca cgcttgcaga aaaaggtatc ccaacggttc gccatcacta ttttctgtcg
     4861 acaaccgagc gaggccgcga ccgggttaat agctacattg tcgagcaatc cattctggcg
     4921 cagatccaac agtttcacga ggatgtcccg aaaactggtg gaggcttaca tacacaattg
     4981 gaggcatgtg ctgcccacta caaagtactg ggaaagccat ttgtgctcat tcttgatggt
     5041 ttggatcatg tctggcgtat caacgctgag gataaacgac cactagatga ccttttctgt
     5101 caggtaattc cctgcccgga aaatatggta ctgctcgtcg gcactcaacc ggtaaatgac
     5161 gaacagttgc cagcggattt gctcgccttt gcaccaaaat ctgagtggca tacattgcct
     5221 gcaatgtctg agagtgcagt actttcttat ctacgtagaa cagttcaaga aggctggctt
     5281 acaactggtt ttgaaagtga agaacaaatt gaagaacaac tgcagagtgc agcatccgct
     5341 cttcgtaaga aaactaatgg ccatcctctt cacgttattt atgctacatc agaattgaaa
     5401 cattcgggtc gcagactatc cagttgggat attgagcagt tgaagggaga cttgagccaa
     5461 gacgcccgat tttactatgc atctctatgg gaacgtcttg ctccaagcca aaaggatacg
     5521 ctaaggctta tctgtgcctt tacttttttc tggcctaaga cagccttctc agagattgct
     5581 accaaaatta aggcggtgga accagatgtg gataaagtag agcatttgct ccattcgtct
     5641 gtggctggtc ttaaagtctt ccatgaaagt ttggctgttt tcgtacgtgc aactgatgac
     5701 tatgagagtc gaatcaatga attaatgcct gttattgcag actggcttga acatcaggca
     5761 ccgacttcac tacgagtgaa ctggttatgg acagttcaag cgaagcttgg taaccccaac
     5821 aatctcatta caggtttaac tcgtgactgg atcatgagtc gcttaaaaga aggatattca
     5881 gaagctttat ttgagatatt attgtcagaa gccctgagcg ctgcgcttga aactgcaaat
     5941 ttttcagatg cctaccggtt ggcgcatctg aaagctcgta tggttgatgg aagtcaattt
     6001 cagatgcagg atagcgatct ggctaggctc atatctttta cattgacact aacctcagag
     6061 gaaagtgtga tacgtgagtc tatagcctcc aggcatgaaa cggatatttt acacgttgtt
     6121 gcacttggtc tggccttact gtctcgaggg gatattattc aggcagaaat ttgcggtgaa
     6181 gaagcctttc atcggttcag agccctatgc caattttcca ataaatataa ttcaagggct
     6241 ggttctgatg aactcttgtt tctggccagt gcatttacac agcttggcgt tatcgctgat
     6301 acatctgaaa atctcgcttg gttagtcgac aaaaatagcc cggacatatg gttacctcgc
     6361 gttcaaatgc tgattcaaaa aggtaatttg gatgatttaa tgttgctagc agcttctcta
     6421 cccgatggac agaaaaagaa cataattagc gatgcttgta tacgagcggc tgcggttgca
     6481 ggggtagtag tgaccgagcg agaggacttc gacgaacttg tcagaactcc atttgtggct
     6541 gcaatagagg tcgcgttaat caaaacctca actccttcga gccaaccaat cccggtgaac
     6601 tggataagcg acagctatta caaacacaaa gataatttgg caactctcac gcaccactgg
     6661 ctgtttggtg ccgtacatct gaatctttgt atgattgcag aagggcaaac aaacttcgag
     6721 cacatcaaag cccctatgta cgaagataga gaagatatta ctgattatct caatgtactg
     6781 tccggaattg gtgcacaaat agcccaacga tggtggtgtg gggaattcat caacttccat
     6841 gagctgtatg aattactcga gcctgttgaa tcgcggcgat ttagacaaag ttattttaga
     6901 aaatcggcaa cggcggactt tcaagcggca ctacaccgta ttgcttgcga tatatatatt
     6961 ggtagtgttc tgctttcggg aactagtgct gtggtacttt cccaatcgac tattgatgct
     7021 gcgttgcaat gcgcttggtt cgatagtgcg tcattccgca ctcagtacgc atctggttca
     7081 ttgatcagaa tggccgacga agcggctata acattcattg gcgctcaacg tttgttactt
     7141 gatgcagaca ttcaaaatga gaccagtgta catcttcaaa taccattaca actctgtgcg
     7201 atagcgatct cccatcaact gaattcggag gctcgtgagc tttgcacgca gacgtgggaa
     7261 ttgaccactg gatacggaca tcgtaaagat ccgacactga gtaatacggt tgatgccatc
     7321 gcttatttag ttgatgtagt gccgaaagaa gcttgtcggt tactggctaa aattgcgccg
     7381 caaatacacc atgttttgga ttacaccgat ggtaagggta cgagacatgt tcttgctgaa
     7441 gcagatcggc tactggcaaa gctgaagcct tccgcattgg taataaaatt tgaagagcat
     7501 acatgtgtgg gtgactgggc tttggccgag agcagtatgc aggcctacat caaacaggga
     7561 atcattgaag agtggcctct ggatgctcta atgcgcacag gccttcatcc cgaaattcgg
     7621 gatacacttc aacaacttgt agaggatggt tgtacaactg cttccgagcg gcttcgcatt
     7681 cttgaagagc ataatggatg ggatattggt tgtttacaac gccacgaaaa ttcaagtagt
     7741 gacgctgaca ataaacctta tgaaggtgat gtgacggtct ttagacctga acaattggaa
     7801 gacttgctca aagttctgga accatattac aaagagcgtc caaaacttct ccgtacttgg
     7861 tatcagcact gggaaagcca aaagcagggg cgaaatttac tcgtcgaact ggataagatt
     7921 ttatttgcaa aaaacaacaa atggatgagg gaagctctac atctttccga gtttgcattt
     7981 caaacaaaac gtagattaag tggcttaact gcagcttgga agtatcttgt tcatgcacag
     8041 atccgtaatg ggggatggat tggttacatg gagaacgtgg atgtgacttg caaaagactc
     8101 gacctggttg tccaaaatta tccgcagcgc tgtgatgaat ttgtggccgt aacaacctac
     8161 aacatgtttg atgagccagt gtcgtcacgc ctagctccat cggaagttat ggtctatttc
     8221 tacgttaagc aaaatcgcat tgatgaagct gtaaagttca cagagacgat ggttaattgt
     8281 gtgattgagg atactcggac cttgccctta gaaccacctt gctggatgtc aaaactgctc
     8341 gagccaatcg cccaggaagt gaaatgaacg atcttcgcat tcttattgcc cgtcttggat
     8401 ggcctgctac gtctgctcgg tggtggacta tccaggagct tgccgctcga ctgggagagc
     8461 ctgagacgaa agctgaaacc gaatcattgc tgcttcaatt gctgcgctcg cgcaaacttg
     8521 aagccgaggt tgtcgaagta ttatgtattt tctggatggc aattaaagag tttaactact
     8581 ttcctacgac tttgttggca gagaatattc ttctaccctc accactttca tcattgttga
     8641 tgaaagattt tgggttatcg attctgctca tgaatactga tttggaacta gctccagaag
     8701 acttcatcat tcccgatgac tttgatagtg ttcaaggtac ggatttgcca agaatcttcc
     8761 atataaccat tagcaagttg gaaactttta cccgctgtcc atttattcga caaatggcct
     8821 tcgaatggac aaaaaaccgg acggcatatc caaatgcacc gtaccaaggg gatgcaggat
     8881 atttcattcg gcccttggga gagggctttg cccctcattt ttcctctcga acagcccttc
     8941 gcgtaatttc cgcttatctt cgcacgttag ctgtggctag acacatctgg aggataccaa
     9001 tggagttgac cgagcaacat tctctcttgg cgttgcctat ccatccaact ttggcttttc
     9061 ttcgacctta tcgtcccgaa tggctctcag tatcgactga attcaaaagt gatagtaagg
     9121 cgatcgaggc ttcattgcga actttctttg ctcaagtaga ggcggcaaac ccaggagacg
     9181 agctaattgc atttagctcg cccatttcaa tgtcaatgga acagtgcgta gagttgtcga
     9241 ttgttcggtg gtcacaaact gtaagcagtg ataaggaagg agcggactcg ttgacatccc
     9301 atattgaatc gttctggaca cgtgaacaaa tgctttccag ccaagctccg gatccactca
     9361 gtacaacaac cttaataact ccaccaactt ttgaacaact ggtgaataaa acttgtatgg
     9421 cttggccatt ggcgggaacg ctcgattttg gtcggatagg atacctacaa catgatttgt
     9481 atccatcccg gttattttta ccgacgatgc caggcataga aaattctctt gaagtcatcc
     9541 cgcatgatgg gcgtctacag gttaagttaa aggacctagt cgtcgcagag ctttattatt
     9601 ggaatgctgg atgggggcca gctaggctag ggcaaattca aggtaattgt gggacagccc
     9661 tcatatcgcg aggcactaca tatcgcgaga gaaacataac aaataccggg tcattgcgct
     9721 cattttactc ttggcaagta cgtagattcc atcgtaatgg aagttatggc agattcagtg
     9781 aaaaaattac gagaggggta gtatttgttt gatgttattt cttaattcat aaggggaact
     9841 tataatttat aacttataac ttataagtga tgtcagttat agataaagta aaaggatatg
     9901 aatattactc atgagccaat ttaaattcac agagcttcat gctatttcgg aaaagccaca
     9961 gaatgatgat gccgcttggt taatagaggc tgaaaggtca attaaatttc tgaaagaaaa
    10021 tgctcaaaat gatgaagtgg ttatctacgc gattggaaat agtgtcctaa ttcatggcgc
    10081 gctagctcta gagaaaaatg ttactacagc agatagatgg gcattgcaga atatgcgtat
    10141 ggatgttgag tattgttgga agattcaaaa gtcatggggt ggaggtcaag gacatcgtat
    10201 ttatcttgag ccacctttag atggggtgtt caaaggggga gaggcattaa tttatcgtcg
    10261 taggtttcat ggcgttgaag aaggccgtgc acctatcgaa ttaagtcaga aacttgtgca
    10321 cagccttggg ttatattact tgccagaaag aaatgcatat tgtcgtctcg atgaacgggg
    10381 tgatattgaa gatgtaatca ggataattaa caaaaatatt tcacctcctt ttaactatct
    10441 ggatatagtt actattaaga gaaaggattt agatacattc atggctttat ccaaaacatg
    10501 tcttgtttta aagtttgatt ttacacgtgt cagatggggt aattttgctg gctgggggaa
    10561 tatcaacaga tatactaaag atgaagggag tcttttttat catggtggta ttaacgggga
    10621 agccagttac tgtaatggtg taatgatcat tcgccctcaa gtaactgtgc gcggtctggt
    10681 aaaagcatgg aaggatgaag gtaatccatc taaaaggaaa tacgcaacct ttaaaatata
    10741 cgatcgtaaa aatagctgca atgttgaaac atcttgcgga ccagactcac tatctaacta
    10801 ttttcaagaa aataaattac cgtgggaagt ttcacctgcc ttttttagag cagaagtgtt
    10861 acatcgtttc aaggcggatc cagaaaaata tactttagat gaaagaagta ttgcttgtcg
    10921 aaatgcttgg tatttaaaat cctatgatat taatgaggcc gggcaagttc atgtatacat
    10981 tggtgatttg gctcatttac cttatgaaga acagctctat tggcaagcct tcaatgaatg
    11041 gccaaaagga acaatttcta agcgtgcatt tcagacggat attgttgggg aatggtatct
    11101 gtccaatgaa ccattgttgg cactcaaaca caaaatttcg ctaatggaca agaatcctcc
    11161 cacctggtgg aagccacggg gtacagtatt gagtgacgct gttcagtatc ctgcaacaga
    11221 ttcaatcaaa gagtggggtg atgaaatcct tgcgcttgat cagtatttag ttgagggctt
    11281 tttaccaaag ccactgagtg aaattgctaa atatgagggc cgaatcattg aacccaaatg
    11341 gggatccttg cgcatactac aagaagctct tattgctaaa agtgcaacag tagatgaagc
    11401 gaaaatgtta gttcagccta tgcaaaaact gcatgctcta cgaaccgaag tacgcggaca
    11461 tgcttctaca gaaaaaaaga agaaagcgat attagaagcg cgtaccgatc atgaaaatct
    11521 acgtgcacac ttcactagcc ttgtggggca atgtgaagca tcatttaatg atattttaag
    11581 gatttttgat tttaaaatag atgattagct atttttgtaa tagatgataa ttggggggta
    11641 taatacttgc caatcttagt catagttgtg tgagacttat cgagcgggtg agaggtcagc
    11701 ttcaattatg aaaatgacc
//
LOCUS       DAZUCE010000156        11702 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZUCE010000156 DAZUCE010000000
VERSION     DAZUCE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.156"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..631
                     /locus_tag="ACHY03_003551"
     CDS             <1..631
                     /locus_tag="ACHY03_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3906738.1"
                     /translation="TVQVIELAVNPTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGD
                     FLNREYDAVIMRENFCAPWAEVEYLFDEEILPVCSGSLLAMSDQKLSVAELLTELPLL
                     HQSTRITGWEEWFALSGVSSPLVNNGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLD
                     RGDMVIPCDVPIRTGNRFIMTWQEEKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(665..1267)
                     /gene="cobO"
                     /locus_tag="ACHY03_003552"
     CDS             complement(665..1267)
                     /gene="cobO"
                     /locus_tag="ACHY03_003552"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFS3906739.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1277..2929)
                     /locus_tag="ACHY03_003553"
     CDS             complement(1277..2929)
                     /locus_tag="ACHY03_003553"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFS3906740.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKIAKLASTKYYDNLPTSGNEQGQAFRDIELEKALL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMLDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGESMPDYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3037..4317)
                     /locus_tag="ACHY03_003554"
     CDS             complement(3037..4317)
                     /locus_tag="ACHY03_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFS3906741.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTVSPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWEGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4478..4795)
                     /locus_tag="ACHY03_003555"
     CDS             complement(4478..4795)
                     /locus_tag="ACHY03_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HFS3906742.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(4792..6162)
                     /locus_tag="ACHY03_003556"
     CDS             complement(4792..6162)
                     /locus_tag="ACHY03_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HFS3906743.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AQEEHLSFSMALIPADVDKAIVHQALDNGKITALEFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6166..7407)
                     /locus_tag="ACHY03_003557"
     CDS             complement(6166..7407)
                     /locus_tag="ACHY03_003557"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050096 - methylaspartate ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HFS3906744.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7407..8852)
                     /locus_tag="ACHY03_003558"
     CDS             complement(7407..8852)
                     /locus_tag="ACHY03_003558"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0019670 - anaerobic glutamate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HFS3906745.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVENFEDGVKYQQTIP
                     EHKRFSLALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARFEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(8871..10259)
                     /gene="glmL"
                     /locus_tag="ACHY03_003559"
     CDS             complement(8871..10259)
                     /gene="glmL"
                     /locus_tag="ACHY03_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase accessory protein GlmL"
                     /protein_id="HFS3906746.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNSGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARHAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10259..10708)
                     /gene="glmS"
                     /locus_tag="ACHY03_003560"
     CDS             complement(10259..10708)
                     /gene="glmS"
                     /locus_tag="ACHY03_003560"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050097 - methylaspartate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HFS3906747.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVETKFKEMGFDRVFAPSHDLEDVCQLMAHDINQRHDVDTRILEEAI"
     gene            complement(11256..11678)
                     /locus_tag="ACHY03_003561"
     CDS             complement(11256..11678)
                     /locus_tag="ACHY03_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906748.1"
                     /translation="MIPSQISFNTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEISSDT
                     VIFSILSSLIDSAISKFQHQNPVNGSVNPEILYEDYTD"
BASE COUNT         2807 a         2880 c         2907 g         3108 t
ORIGIN      
        1 tacggttcag gttattgaac ttgccgttaa tcctactttt agtacccact ggttgatccc
       61 aaatttacat gaattcacaa agttacatcc cgatattatt gttaatattc attctttagc
      121 taataacggc gattttctta atcgggagta cgatgctgtc attatgcgcg aaaatttttg
      181 cgcgccctgg gcggaagtag aatatctctt tgatgaggag attttgccgg tatgcagcgg
      241 cagtttgctt gcaatgtccg atcaaaagct gtcagtggca gaactgctta ctgagttacc
      301 gcttctgcat caaagcaccc gtattactgg ctgggaagag tggtttgctt tatctggcgt
      361 tagcagtccg ttggtcaata acggaccacg tttcgatttg ctttcaatgc tgattgccgc
      421 cgtgcgctct aatctcggcg tggcgctgtt accacgtttc gctattcaac atgatttgga
      481 taggggcgat atggtgattc cctgtgatgt gcctattcgt accggtaatc gttttattat
      541 gacgtggcag gaagagaagt ctgactcacc gcacttgcag caatttcgtg aatggttgct
      601 ggcgaaatcg gtcgtaccgc aggagatgta aaacctgcgc ctggcgggat gccaggcgca
      661 gttattacca gtcgataccc ggctgggcct gaatgccgtt atcaaaagca tgtttgacgg
      721 ggcggatttc gcttaccgta tccgccattt ttaatatctg actgtggcaa ccgcgtccgg
      781 tgacaatgac actttgttgt gccgggcggt tttgcagaga agcgatcact tcttcggtat
      841 ccaaataatg gtaagccagc atataggtca gctcatcaag caccactaag tcgtaacgct
      901 tgtcagccag catccgtttg ctttccgacc agacctcttt cgccgcgtcg atatccgctt
      961 gccggttctg tgtctcccag gtaaaaccgg ttcccataat atgaaactca acccccagcg
     1021 gctggagaag gttgtattcg ccgttgtccc attgcccctt gatgtactgc gcaaccccca
     1081 ccgttttgcc atgacccacc gcgcgcgtca cagtaccaaa cgcggcagtg gatttccctt
     1141 tgccgttgcc ggtgaaaaca atcaagatcc cttttttctc tatcgccgcc gcgacgcgcg
     1201 tatcgacctg ttccttcaat ttctgttggc gctgcacatg ccgttcagtt gaaattcgcg
     1261 cttccattca ttttccttaa tgtcccgcag ggcaactggc gcagcggtgt tgctgctcaa
     1321 actgactaaa gaaattattg cctttatcat ctaccaggat gaatgcgggc agattttcta
     1381 cttccatcat ccacaccgct tccataccca gctccgggta ttccaggcaa cgcagacttt
     1441 tgacatattc ttgcgccagt aacgcagctg caccgccaat acttcccaga ttgaagccgc
     1501 catgtttgtg acaggcatcg gtgacctgct ggctgcggtt acctttcgat aacatcacca
     1561 gactgccgcc ggccgcctgg aaggtatcga tataaccatc cattcgaccg cccgtggttg
     1621 gacccagtga accgcaagcc atgttctccg gtgttttcgc cggaccagcg taatagacaa
     1681 tatgatgctt caggtaatcc ggcatcgatt cgccgctatc cagccgcgct tttattttgg
     1741 cgtgggcgat atcgcgcgcc actacgatgg gaccacttaa cgacacgcgg gtgcctaccg
     1801 gtaatctggc gagatccagc atcacgtcac gcaacggacg gtttagatcc agttgtacat
     1861 gttgcgcgtg attttcttcc cgcaatgagg ctggaatata ttgccccgga ttgtgttcga
     1921 gtttttccag ccagataccg tgtttgttaa tttttgcttt gatattgcga tcggcagaac
     1981 aggagagcgc catagctatc gggcaagagc caccgtgacg cggcagacga atgacgcgaa
     2041 tatcatgagc gaaatatttc cccccaaatt gtgcaccaat accaaattgc tgactggcct
     2101 cgagcaacgc tttttccagc tcaatatcac ggaatgcttg tccctgctcg tttccgctgg
     2161 tgggtaaatt gtcgtaatat ttcgttgagg ccagcttggc aatttttaag gtttgatcgg
     2221 ctgaaagccc tccgacgaca aaggcgatgt ggtaaggcgg gcaggcagcg gtgcccagcg
     2281 atttcatttt ttcgattaaa aatgcggtca gtttttctgg ttgcaataag gatttggttt
     2341 cctgataaag cgcggcttta ttggcggagc cgcccccttt attaacacag agaaaatggt
     2401 actcatcacc agcgacagcg ctgatatcaa tctgcgccgg gagattggtt tgcgtattga
     2461 cttcggtata catatccagc ggcgcatttt gtgaaaagcg cagattattt tcctggaaag
     2521 tactgtaaat gcctttgctt aaggcttcag cgtcgttacc tcccgtccat atctgttgcc
     2581 ctttactggc aacaatagtt gcggtgccgg tatcctgaca gttgggtaat accccttttg
     2641 ctgagacttc agcattacgt aataactgca acgcaacgta tttatcatta ctactggcct
     2701 gcggatcgtt aagaatgctg gcgacctgtt gcaaatgagc ggaacgtaag aaaaaagagg
     2761 cttcataaaa tgcctgccgg gcaagcagcg ttaatgcttc aggagcgatt tttatcactt
     2821 cttcgccgtc taattccgtt actgtaatat gttgatcgct aattaatgta tattccgtac
     2881 cgtctttatt ttgtagaaac ggttcttgcc agataaatgg tttggacata tttcactttt
     2941 caccttaaac gttagtgtta cttaattgcc gtctaacttc atgatccggc ttataaaaat
     3001 gcgtagtcat tggcttctga ttgcttaaga tccccctcag ggcagagcaa cgttcgattc
     3061 attgggtttt aatgctttac cccgacagcc ctcgccgaaa tgttcgcctt cccaccgact
     3121 caccatcgca gcgcccattg cgttgctcat gacgtttgtc gcggaacgcc ccatatcaag
     3181 gaacggatca acgcccatca gcaagatcag ccccgcttcc ggaatattga actggttgag
     3241 cgtggcggcg ataaccacca ttgaggcgcg tggtacgcca gccattcctt tcgaggtcaa
     3301 catcaggatc aacagcatgg tgatttgctc accgatggat aaatggatat tgcaggcctg
     3361 ggcaatgaaa actgtggcga aggagcagta ggccattgat ccaacgagat taaatgagta
     3421 gccaatgggt aagacaaagc tggcaatttt gggggaaacg ccaaattgct ccagtttttc
     3481 aagcgttccc ggaaaagccg cttcagaact ggatgtggta aatgccagca gggcgggttc
     3541 cgaaagggcg cgggtcaggc gtctgatgca ggggccgaca taaacgatgg ccagcccgat
     3601 aagcagcacc caaagtaaca acatggtgaa ataaaattca cccataaaga tcccggcgct
     3661 caccataacg gccagtcctc gttcagcaat caatgctgaa atagcggcga atacggtcag
     3721 gggagcgaag agcatgacgt agccagtgag ctttaacatg gcatgtgcca gcgaatctaa
     3781 ggcgtgaacg atggcgctgc ctttctcgcc aatcgccgtc aggctacagc cgaggaaaat
     3841 tgagaacacc acgatttgca aaatttcatt gtgcgccatg gcatcgacaa tgctggtggg
     3901 gaaagcatgc gaaataaata cttttagcgt aaagggttct gacgcgacca ctcctgtggt
     3961 ttcggcaccg tgtgcaacaa aattaattcc tgtgcctggc atgaagaaat ttaccgttat
     4021 caagcctaag gcaattgaca gcaatgaggc gcaaataaat aaaaagagtg ttttagaaaa
     4081 aatacgacca agggctttgg catcgcccat tttagcaata cctaccacca gggtagagac
     4141 cactaacgga gcgataatca tttttatcag tcgtaagaaa atcgtcgtga atatcgatat
     4201 ttcttgcgca tagagttttg ctgtttccgg tgaaacagtg ttattaagca ctacgccgat
     4261 aatcattcca agtaccagcg ccaaaagaat catcgtggtt aaacttattt tcttcatttt
     4321 ttcctccatc aggtgcacgg cgataaatca gaatcaggag aagtagatgc ctgaatagag
     4381 attcaggcat ctacctgcta ttacaacctt acaaatagct gaagtgttga actgcaataa
     4441 gtgacgggat gtcagttggc taaaagtatt cggattatta caaatcgaaa ctcatattta
     4501 ataaaggtgc atgttgctgt gcaccataga catcattgtc acctacattc ccggagataa
     4561 tatctcgtgg catgacgatt tttactgcgt tagaaggatc aaaccagaca atacgatgaa
     4621 taaagtctgg ttcaacatga tataagcgcg caatcagttc tggggttaat tgccctgatg
     4681 atttaactcg ctcatagttt tctttcgttt taaataaaat atctaaaacc agttcgtaag
     4741 gtccggcatt tttcgaacga atgacttgcg ccagtgagac aattgactgt ttcatgcctg
     4801 aactccttct ggcgtcacct gttcaatatg gaaatcaaaa cgcagagcgt cgttggcttc
     4861 aatcaggtga tatatggaaa attcgtatac cggcccgccc tggatatcag aaggggagaa
     4921 cgggaacgcc agattacctg cggtagcgat gcggttttca tagccgtagt gcagcatggt
     4981 agagcgcacc agcgagcaaa cgctgttggc aatttcctgg gtcggtgcaa ctacatcaag
     5041 gacaatcccc agctcatgcc cggcagtttg catcggttca tggtctccca tcaccccgtt
     5101 tttgccgtac aggtggaaat tgatgcgaat gctgtcatcg tcgagcgaca ggttacgcga
     5161 aacgctggtt ttgacttcat cgatgatttt atcgatcccg gcgatcataa tcgggtcgcg
     5221 cgttccggcg atggtcagac agcggaagcc cacccgtcgt gcaccttcca gtttcagggc
     5281 atacggcgtt tcttcatgct tagaaccgct gacgtagact tcgccgtcat tgactgcttt
     5341 aaatgtgcag cctttcaggt tcaacacgcc gccaggtcct ggcaggaagt agggatcgga
     5401 tttctcatac agtgtgtgtg cagctgctga cgtttcggta aatttacgct tcggattaaa
     5461 tgtcttcagc gtaaagccgt tgtcatcaat gatgcccatc gcacagtcag agcctgagcc
     5521 gggcgttgcg gcgattgctg cgcactcaag gattttccca caatgcagcg ccagaccttc
     5581 atcaaagcct tgcatgatcg ggagcgcagc aaagcaggcc gggtcgtaag cgcgcccgcc
     5641 cagcaccact tgcgcgcctt ctttcagcgc ccgctggaag ggttcgatgc ccatttgcgc
     5701 gacgatatac gtactttcgt caatcgcgtc gtgggttaac ggcgggacaa actccagcgc
     5761 cgtgattttg ccgttatcca gcgcctggtg gacgatggct ttgtcaacgt ctgccgggat
     5821 caacgccatc gagaacgaga ggtgctcttc ctgggcaatc tcatgaatta tttctcggca
     5881 ccactccagg tgtggtgcgg caccagaacc gccggccgtt ccgatcacca ccggtatatt
     5941 attttgcacg cctgcggtga tcatatagcg cagatcgcgc ttgacccctg cgcgatcggt
     6001 aaacggtttg cctgccccga ggtagtgggg gcctggatcg gaagagcctg catcaacggc
     6061 gatcagatcg ggcgattctg ccatcgcttt gcggaagctc tcttccggga atccataccc
     6121 gaggattgcc gttggcgata agactttaaa agaacgagcc atttgttaat ccttagcctg
     6181 caacagcgcg atagtacgat tcatttcgtt gaagacaata tcgaggcctt cgtcaaagcc
     6241 catccctggt tttaccagca tacgcatagg gcgagcggca agggcgacgt ggacacaggt
     6301 gcgggcactg acatcagttt cattgcaggt gccgccctgg tacgcttcca tgctgtggct
     6361 gttgcagtaa agaaccgcat cgacgatgtt gtgaatactg cccagatccg gggttttgat
     6421 ttgcaccatg tgacaactgg cagcatcagt gaaatcaaca atatcctggt aggtgttaca
     6481 ccattcatcg gccacaattt tcacgccgga accgaggcgc gtcagctctt tggtaatcgc
     6541 ggtcagcagg cgaatttgat cgggcttgtt accggcatcg accggccctt cgatgtagag
     6601 cggcaggcct tgcgcttctt tttccaggct ggcgatgtat tgcgcacagc gaagcggatc
     6661 catatcgaag atcagaccga tagtgccgta tacatcgatg tgcagggttg ggtggtagcg
     6721 tgcgctggtg cgcttgctca gaatgcgatc cgacaaccag cggacatatt cgcgcagttt
     6781 ttcaccttta aagcccagct tctcttcgac gttattaatc agcgcatggg gcagcacgtc
     6841 gatgccttta aggatcatct tatcgacggc gatatatcga tcgtcgccgc tctgaccaaa
     6901 taatggaatg gattccgcca cgcgtggcaa ctgccattca tcacagacca cttcagtttt
     6961 cagacggccg gttgccagcg cggtagcatc aagcagcgcc tgtgataatc cgtagcgcac
     7021 ggcggtatgc agcaagttgc cgtcaatacg caatttgtcg aagaaacggg cattcggcag
     7081 gaaagcatcc acatcgcggc ctaccagtaa tggcttgata tggtcgttga ggaacggaat
     7141 aaagtgctct gcgaggaaca gtggatcgcg accgcctgcc ccggaatact gtacggcagt
     7201 acagtcacct acggcgaccg cgccgttttc cagaatcaac tgtaccgata cgcactcccc
     7261 ggcctgacgt acggcgttaa acccctgtgt tactggctcc ccggtataaa aaaagccgtc
     7321 atgacccgct ccgtttttta tcgcctgctg gtcatcgaaa tagaatgagg agtagccagc
     7381 ggtgaacaga gcctgtttta ttttcattat ggtcttccta ttaatttact gtgggataca
     7441 gcattgatgt catctacaac catctggaat gtcggttggc gtccttcaaa gcgtgcgcgt
     7501 tccgcaacgt aatcgtggtg gaggtcgaga atatcttttg gcaatggaac ggctccggct
     7561 tccagtacgc gtatcgcgcc ggtgttgtca cggaccggaa gaattttgcc cgcgttgcag
     7621 gcggctggcg caaacggcac atccagtacg cccgcttcaa atgccagcac cgtaccgcgc
     7681 gcaatatcac cattacccag ttcgaacact ttattcagta ccgcgcgaac ctcgctctta
     7741 atcagttcaa tttccagttc aaccgcaggg cacggcggga atttctgctc gttgaccatg
     7801 ttgagcatct ggcgggaggc tttcagcccc tgaatgttgg cagcggcagt cgggatgccc
     7861 caggcttcgt gcggactttt ggtgatgact ttggtggcac cagacatccc cgcgacggct
     7921 gcgccccatg agataatagc gaacgctttc gattcgtctt ccggaaagcc gcccatccac
     7981 tggtgaaaca cggtactcaa ttcatagtcg gtatagccat agctctgaaa atattcgtgt
     8041 gcaagttcac gcagagactg gatcgccgca acatcctgcg tcaggctgcc gacctgtcca
     8101 taaccaacgg tgatagattt cacaccctgt tccagcgcca gcaagccttc gattatcgcg
     8161 atagagtgcg agataaacgg cgggatcagc gtacctgtta gtgggccaaa cggttcgcgg
     8221 ttgatgcgga tcccgtgttc ttcatacatc cccattaaac ggtcgcagta ctgccagtcg
     8281 cggatcgatt tttccagcgt tacgcgtttg gcatacggaa tgttgtagga aatgccaccg
     8341 ccttcataac tggtaaagcc gctggccatt gagatttcag ccagcagacg cgcatctggc
     8401 gtgccgtggc ggatctgtag cggtttttgc agggtttcgg tgaggcgacg gcaagctgcg
     8461 acaccatggt taactaccgg taaaccgttc agtttagagg ttccggcttc gatggatttt
     8521 ttaatcccta cagccgcttc ttcgtaccga ttcagacggg tatatgcatc gatggtgctt
     8581 ggcagcagat cgcactcttc ttgtagtgtt ttcagcagtt cgatgtgttc gtccatcagc
     8641 gccacacctg cgcgcggttg gctaagggtt ttaccttcct tgtctgcttt cagcagcgca
     8701 agagagaagc gtttatgctc agggatggtt tgttggtatt taacgccatc ttcaaagttt
     8761 tcaacatcct tgccggtttc ccaggttttc agtacctgtt gccgctcggt catgaattcg
     8821 tcatgggtta atttcttatt tcgaagttcc attgattgca gccttaattg ttatagggtt
     8881 aagcattgaa tactggtgcg cgcggcggcc agcggatcga ctctggcgac gtttgccagc
     8941 agtggtaata gcccccggga atcgcggtaa tattcaaatt cggtcggtaa gagaatgcgt
     9001 ttaccgtcgt cgtttaactc gcggtatttc aaccagtgat gcatatcgaa ctgactggca
     9061 cgcgagagcc atccgccgga tccgacgact ttacgcacgg ttgtcaggtc acgtcccatc
     9121 tgtaaatcga cattgccgac gcaggtgcag acctcttttt tggtgcccgc atgtctttcg
     9181 gtggcataac ccacacacag tccggccagg acggtatcga aatatttctc ttctgcattt
     9241 gcgggtaagt agtcgggatg agcgactaga tgacggaggt aatgataaaa agcgtccagc
     9301 tgtgcgggtt gctgagcaaa attcacattg actaactctt tcgcactttc accaaccacc
     9361 actgccgaga cgcgcattcc cagatcgcct tccaccgtcc gttttacgaa gggttccggt
     9421 acgccgtgta aaacggtgtc gggcgagagc gtgttggcac aggcggaata aacatcggtg
     9481 gttgcgccgc ccatgtcgat cagcatgaac tctttccagc tgtgatcgac gttactgatt
     9541 gccttgacca gttcaaagac cgtccatggt gtcggcattg gttcttcgcc tgttttatca
     9601 acaatgacat ccagcccttt ccctttaacg atgcgtttaa ggaatatgtc gcaaatagca
     9661 tggcgtgcgg ctaaagggtt cggatggtca agatcgggca gaacattgtc gacgatggtc
     9721 aggtctttat gcccgaggat ctcctgcacc tcgtcctgaa tatcccggtt tccggcataa
     9781 ataatggcgc agtcgagttt ggattctgcc agcacacggg cgttattcag tccgtagctt
     9841 tcttctccgc cgtcggtgcc gccggtgaac aagagaatat cgggctgcgt ttcttccagt
     9901 gcctggatat cacggcggtt cagcttgtag gcgtaatatt gcgcaatttt agcacccgca
     9961 gagtgagcgg ttactttcgc ggtttccagg gtgatagaag gcaccaatcc cattgcggca
    10021 accgccagcc cacctttggc agaggaggaa tacttcaatg caacctcacc gctgtttaac
    10081 agggggaggg cattatctac gtttaacacc tggttcaggc tggagaaaaa ccccttcgcc
    10141 agatgatggg tggtggttgg ggtcagtacg tggttaacca acgttaacgc gtccccctcc
    10201 cgggtgaaga gggctgcttt ggtccacgtc gatccaatat cgacagagac gatttgcatc
    10261 agatagcctc ttcaagaata cgagtatcaa cgtcatgacg ttggttgata tcgtgggcca
    10321 tcagctgaca aacatcttct aagtcatgac ttggggcaaa gacgcgatca aatcccatct
    10381 ctttgaattt ggtttccacg tcagcgaagt catgttttcc gaccaccagg ttaccgccca
    10441 catagagcag gatgtccccc agtccacgct cgatgcagcg ttcgcgcatg cccagacagt
    10501 cgatatcgcc atgcccatag atagaggaga cgacaatcgc gtcagcgcca gtttcaatgg
    10561 ccgcatcaat atattcgtcc tggctgacca ttacgcccag attaatgact ctgaagtcgt
    10621 ggttgctaaa aacgcggtcc agaactttat tgcctactgc atggcagtcc gcgccaataa
    10681 cgccaatcac aagtgttgct ttcttcatgg gtaatccttt gtaagacaga gagtaaaata
    10741 agttacacac gtcgtattta tggccgttca taacggcaca ggtttaatat cacaggagtg
    10801 tctttatctg accgtaatgt tgcaattgat ctattttcaa aatgtctttc gggaaatgat
    10861 tgtttaaagt atgtttttaa attctcatgt taattcagtg tgttatgtca actaatgtca
    10921 tgaatgttaa atttttaaaa ttaacttgat ttggtgtatt gagataattg tgatctactt
    10981 ctaatttgca tttttgtgac gtggttttag ccatgagtaa atgcatttca taagatttaa
    11041 gtcgaatttc actgcaattc atctctgctt aatgtaaaaa taatgttgaa aataacaaat
    11101 ggttaatgat tcatgcgaat tatgacctta gttagctctt ttgatttttc gtaaaaaaga
    11161 gaggtacatg aaggtgctac cagtatgagt aacgttaatc aaaggtttta ttacgtctgg
    11221 caataacgca ggccattttt cgcgacaggg tggatttaat cagtataatc ctcatacaat
    11281 atttcaggat taactgaacc gttaaccggg ttttggtgtt gaaatttaga aatggcggag
    11341 tcaatcaatg aagacagtat cgaaaaaata actgtgtcag aactaatttc aattaatcct
    11401 tctcgttgct gaccaaaaaa gatatttatc ttgatttcat ttgtactacg gcaatcaata
    11461 tgcagtaatt tttgatcgct gagattttct gtttcataaa tgtttttggc gatagtcgga
    11521 tgtattgatg ccagacatct gctcagataa gccagatcct ttagggaaca ccccgctgag
    11581 tagaggatgt cagagaattt ttgtgtgtca actgttggct ctgaagcgta tgttgcattg
    11641 acaggaagag tattgaaaga gatctgagat ggtatcatta tttaaatctt tccgtgagta
    11701 at
//
LOCUS       DAZUCE010000157        11592 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZUCE010000157 DAZUCE010000000
VERSION     DAZUCE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.157"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            318..623
                     /locus_tag="ACHY03_003562"
     CDS             318..623
                     /locus_tag="ACHY03_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS3906749.1"
                     /translation="MARVKRRILAKRYAPIELISVAVSRVAVELRTRVESWPPRLKKV
                     WPEMPQEASSVLREELAIALNELADIRVDFSDYDVSDIERDLDRVESLARDDTDDGG"
     gene            598..2475
                     /locus_tag="ACHY03_003563"
     CDS             598..2475
                     /locus_tag="ACHY03_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012138691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFS3906750.1"
                     /translation="MTIPMTGVEWADKYFYLPEGSSHIAGHWTTQPVQVVMLNMMTND
                     AIKIVSVRKSARLGYTKILVAALLYFAEHKKRSAVVYQPIDDESDGFVADEVDPAIAE
                     MPVIQKIFPDWDKSNERNNLQRKEMSGAIIDFRGASTPGNFRRLTKQVVEGDEVDGWP
                     LEVAKKGKGEGSPIELALVRIKGAAYPKAIFGSTPTVTGKSHIEMLEDAADLTFRFYL
                     KCPHCGEEQVLVFGFDGIGYGLKWDNNLQTNEAKSSSAYYQCCHCPEHFYYRDLEKME
                     LSGRWIAEDCTWTRDGIHFFDHDGGVVRAPKHAAIVINALYSLNLDGWGEIVSEWLKA
                     KGDPLKEKTFHNTTLGELWSDVASEQLEHDILVNRREKYASQVPDGVVYLTGGIDSQT
                     SGRYECYVWGWGAEEECWLIDKTIVLGRYDEEDTLQRVDGVIRKQYRRSDGTTIGVSR
                     WAWDTGGIDAQVVYNRSLKLGPLWVIPIKGASSYGQPVVNMPRTRNANKVYLSLIGTD
                     TAKDLLAMRLPLEPDSKSATPSAIHFPNDDEIFGTTEAKQLVSEVLIPKLINGRVVYR
                     WDNQGRRNEALDCWVYALAALRISKIRFQLNLETLAEQRKKSQNKLSLEEMARMLGGS
                     S"
     gene            2475..2705
                     /gene="gpW"
                     /locus_tag="ACHY03_003564"
     CDS             2475..2705
                     /gene="gpW"
                     /locus_tag="ACHY03_003564"
                     /inference="COORDINATES: protein motif:HMM:NF014846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFS3906751.1"
                     /translation="MSREVLTERLLEAEIALHKLLTGRSTVSLSHGDSAGNNRSYQYS
                     QASIEQLRTYIIELKSQLGLSTGRRRPVGVRL"
     gene            2702..4273
                     /locus_tag="ACHY03_003565"
     CDS             2702..4273
                     /locus_tag="ACHY03_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS3906752.1"
                     /translation="MTAQQLLGPDGKTPLRRYAGYNGGGPGFGGQLIDWNAPQQSADA
                     ALLPNFYRGNARADDLVRNNGVASNAVQLHQDHIVGNLFKLSYRPNWRYIGISREDAR
                     ALARDVEVAWTEYAEDPHCTIDIERKRTFTMMIREGVATHAFNGETCVQPVWESCAGS
                     VFRTRFKMVSPKRIRNPGYAADTQFRRAGVDIDKNGAAVGYWIAEDTYPLGGVGKCRR
                     IPAKLSSGRHAFIHIFEPLEDGQTRGDNIFYSVMERLKMLDTLQQTQLQSAIVKAMYA
                     ATIESELDSQQAFEYIAGAGTDIDSNPLSSFIQSYVTYYNGANIKLGGVKVPHLHPGD
                     KLSLQTAQNADAGFSSLEKSLLRYVAAGVGASYEELSRDYSQVSYSSARASANVSWRF
                     FMGRRRFIAARQASLMFCCWFEEALARGVITLPRSAVRSFYEARNSWTNALWIGAGRM
                     AIDGLKEVQESAMRITTGLSTYQNELALQGQDYEEVMEQQEYEIQRRREMGLSEPSWS
                     VSHPSNTNDNGWGGN"
     gene            4273..5583
                     /locus_tag="ACHY03_003566"
     CDS             4273..5583
                     /locus_tag="ACHY03_003566"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFS3906753.1"
                     /translation="MPWNNFPHLAARAFNQPLLLEPAYARVFFSALSDRFGTGRLIDT
                     ASGEVMNSDEMNALAMGWDSSERTRQKSYRVERGIAVLPVTGTLVHKLGYINPVSGMS
                     GYDGIAKRLQQAISDPDVKGILLDIDSPGGEVAGAFDTADLIARAREQKPVWALASDT
                     ACSAAYLLASACSRRLITQTGTVGSIGVLMAHRCVEKALEIAGVDVTLIYAGAHKVDG
                     NPYSQLPDDVRDEFQLSINSTREQFAQKVSDYTGLKKSRVLATEAAVFIGADAIKSGL
                     ADQLVNYADAIAVMADALKPKTERFMPGTTETTAETTTTEQTAATTTVAPVESNAEQI
                     RADAASSELARVMAIINCPEAVGREAQAKALAAVPGMTVGQAQAVLAAAPQTAQARTE
                     TALDTLMSTESPETIQDAGSTTATGTTANVSMLVAAGRSILGDE"
     gene            5583..5912
                     /locus_tag="ACHY03_003567"
     CDS             5583..5912
                     /locus_tag="ACHY03_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFS3906754.1"
                     /translation="MTTETYSPDDFILGPDLVVTTVGHFQGGINVPRLTPIMLDATAG
                     TFKVWDGSIGKAVGLTATAVNTGSSSADCSYYKSGSFRYTAINWGTVTDVAKRKSAFA
                     GTPVSVG"
     gene            5973..7007
                     /locus_tag="ACHY03_003568"
     CDS             5973..7007
                     /locus_tag="ACHY03_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFS3906755.1"
                     /translation="MSDSFTTSELITATQQVFKFNPLFLKLFFRETYTFTSEEVFLDK
                     IPGKVNMAVYCAPMITGKVDRTRGYSTNHFKPGYTKPKHTINPNMSIKRAAGEQIGQP
                     ETPVERRAKIIMQNLLDEELSISQLEEFQAVQAVLYGKYTVSGSNIETYEIDMSRSAT
                     NNVTQSGSTAWSTQDAETYDPSDDIESYADLASGAVNVIIMDGKAWKQLKRFKKFWTA
                     LDTRRGSNSQLEVALKNLGDVVSFKGYYGDTALFVYKGQYIDPLIGTETRYMPDNTMI
                     LGNTKNRGLRTYGAIQDEDALKEGICEATRYPKVWTTTGDPAVTQTMTQSAPAMVLTD
                     ADAFVVVKIA"
     gene            7040..7459
                     /gene="gpFI"
                     /locus_tag="ACHY03_003569"
     CDS             7040..7459
                     /gene="gpFI"
                     /locus_tag="ACHY03_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFS3906756.1"
                     /translation="MSTKTELLARIDDLSAQLGRELPRSGTIAELESIVAGAESELDI
                     LNEQSGDAGGIEITVNAAPGTDSDLAQSIASAIVSQSQEPELAPATHRVKLRNTLDVY
                     HYVNGRRVREIVAAGREIVVDSPEVADLIAADHVYAL"
     gene            7456..7836
                     /locus_tag="ACHY03_003570"
     CDS             7456..7836
                     /locus_tag="ACHY03_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906757.1"
                     /translation="MSYYDDLRAGDEEMIREWGRPVKLRGKTDPIIAIFNEPYARVDV
                     PHAGFITGTVTSLTALSDDVAGVVARDVVQVPKQRSIAADGSVTWSDWTDYVVKEPQP
                     DGIGLTNIFLEPHTSSENSEYSKY"
     gene            7868..8548
                     /locus_tag="ACHY03_003571"
     CDS             7868..8548
                     /locus_tag="ACHY03_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906758.1"
                     /translation="MADLRSNAQMFDIDVSALEQLRVEISATQHQMLMAYNRALNRTA
                     KYMHRISAGMILTALAAKNHKAVNKRIKPFIKRRNFTKEGAGDLSSVKLWYGLNDFRV
                     SELKGRLQNPRKQKQPRNPETGQFLKTKKGARGAAFTPKSAGLAMMSWPDSFVAKRYG
                     AKSVWIRLARGGIEEARVPVHDALEDAIDDYIFENIGSVFMGFFEKDLRGRVKGNVHV
                     DPKTGKRL"
     gene            8545..9081
                     /locus_tag="ACHY03_003572"
     CDS             8545..9081
                     /locus_tag="ACHY03_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906759.1"
                     /translation="MSGLDAFDEYLDRVKGAVLQIPFIKTFGIYPEIPAGFETPALFL
                     EISNWSQSDEAVPGSIQSVELSCNLYLLREFAADQYGLKSQNAALYMTSWIDGRMFGP
                     GTKPAKFSDAEPCDWIKNGQSVGSHSVQCVSFTQVVGVGPDIFDYPSQGTLKNIYVGI
                     APDIGAEHESDYYGPIGR"
     gene            9062..9964
                     /locus_tag="ACHY03_003573"
     CDS             9062..9964
                     /locus_tag="ACHY03_003573"
                     /inference="COORDINATES: protein motif:HMM:TIGR01644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFS3906760.1"
                     /translation="MAQSDDEYAAAENARRLRDAVKRGTIAAVKMNPPRCRVSFGGEH
                     QSGWLQWFTHATSERVDWSAPSVGDPVTVVSEGGDTRNGVVMLGLHIDNKAPPSNDPH
                     DHVTAYCDGATMTYNTKNHTLTWQGVPDGVVKILGESEIEIFGRADVTINSENVVNIH
                     GGKLINADADIINVTATDTINAHADLVNVIATSSVSVTAANRISLTAQTISAWAPGGI
                     TLAGPTHITETLVVDKLATFRNDISVTGDNGGTGNITTRGSVLAGQEVRDRQGTMNEM
                     RITYNGHTHICPDGETEKPNQPMA"
     gene            9967..10308
                     /locus_tag="ACHY03_003574"
     CDS             9967..10308
                     /locus_tag="ACHY03_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFS3906761.1"
                     /translation="MLGMNRDTGKPLSGTDHICQSIVDILTTPLGTRVMLPEYGSKLF
                     DLVDNPTDPSLAMRIIMESAGAIARWEPRVRIDRINVLAVDIGKITILIIATDIETQQ
                     RLEFNNMELIF"
     gene            10305..11225
                     /locus_tag="ACHY03_003575"
     CDS             10305..11225
                     /locus_tag="ACHY03_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFS3906762.1"
                     /translation="MITSTVQNSIVKTIDMSLLPPPAFVKTPLFSDVKSNLLSELQIL
                     YPQFNALLESDPAVKLLEIVAYREIIITARVNQGMLAVLLAFAKNSDLDQIGANFDCL
                     RLLITPANPDSVPPTEAVYESDDEYRHRIQLSWYARNTAGSTNAYNYFALSSDPDVLS
                     AQAYGPPVTQPGYVDMYVLSRTGDGVPPQSLLNTVNAALSPDDTRPLTDFVTVKPASN
                     LNYRVEAVIVAGLGPDQNVLLNGAQSDLEVYVTTQHKIGATAALSGIYDAIHRDGTER
                     VILISPTEDAIAGVGQAPHCTEIKLSVQMG"
     gene            11228..>11592
                     /locus_tag="ACHY03_003576"
     CDS             11228..>11592
                     /locus_tag="ACHY03_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFS3906763.1"
                     /translation="MTSQSVLPPNAVTPERALEAVLSHVGDLPGDIRIIKNPDLCPAN
                     LLPWLAWEYAVTYWNPDWSERQKREIIKAAAWQNKHRGTRGAVERALLTVGFNSKMRE
                     WFEATPRDDPYTFAIKIYLL"
BASE COUNT         2994 a         2894 c         3192 g         2512 t
ORIGIN      
        1 actcattttt agtatttttt cattttatgt atttccggtt ccggtgagga tttttaatgg
       61 caagtcaggc tgaggtcgca gcacatttac tgctatcaga tcgtcgtctg cgcgatctcg
      121 caaaacttcc gggagcacca gtcccacaag gacgtggtga ttgggagcta gacgcctggc
      181 gccatttcta tattcattat ctccggagta atagacgcga cacagttggt actgacgaac
      241 cggaagtggg ggacaattct cccgaaaaaa atcgcgagca gtggctgaaa aatgaggaac
      301 gacaggagcg aatcctgatg gctcgcgtga aacgccgcat tcttgctaaa cgctacgcgc
      361 caattgaatt aatcagcgtc gctgtatctc gcgtcgcagt tgaattacgt acccgtgtcg
      421 aatcgtggcc accacggttg aaaaaggtgt ggcctgaaat gccgcaggag gcgagcagtg
      481 ttttacgaga ggagctggcg atagccctga atgaactggc agacatacga gtcgacttca
      541 gcgattacga tgtcagcgat atcgaacgcg atctcgacag ggttgaatcc cttgcgcgtg
      601 acgataccga tgacgggggt tgagtgggct gataaatatt tttatctccc tgagggctcc
      661 agccacatcg ctggccactg gacgactcag ccggtccagg tagtgatgct caatatgatg
      721 actaacgacg cgataaaaat cgtgtctgtt cgcaaatcag ctcgtctcgg ttatacaaaa
      781 atactcgtcg cggcgctgct ctatttcgct gagcacaaaa aacgtagtgc cgtggtctat
      841 cagcctatcg atgacgaatc ggatggattt gttgccgacg aggttgaccc cgctatcgcc
      901 gaaatgccgg tgattcagaa aattttcccc gactgggata aaagcaacga gcgtaacaat
      961 ctccagcgta aagagatgag cggcgcaatt attgattttc gcggtgcgag tacaccgggt
     1021 aattttcgtc gactgacaaa acaggttgtt gaaggcgatg aggttgacgg ctggccgctg
     1081 gaagtagcaa aaaaaggtaa aggagagggc tcgccaattg aacttgcgct cgttcgaatt
     1141 aaaggcgcag cgtacccgaa ggcaattttc ggctcgacgc caacagttac cgggaaaagt
     1201 catattgaaa tgctggaaga cgctgccgat ctgacgtttc gtttttacct gaaatgccca
     1261 cattgtggcg aggagcaggt tctggtattt ggtttcgacg gcatcggata cggcctcaaa
     1321 tgggataaca acttacagac caacgaggcg aaatcgtcgt ccgcatatta ccagtgttgc
     1381 cactgcccgg agcattttta ctatcgcgat ctcgaaaaaa tggaactctc ggggcgctgg
     1441 atagccgagg actgcacctg gacgcgggac ggtattcatt tttttgatca cgacggaggt
     1501 gtcgttcgcg cgccgaaaca cgcggcgatc gtgataaacg ccctgtattc actgaatctc
     1561 gacggctggg gcgagattgt cagcgagtgg ctgaaagcga aaggcgaccc actcaaagaa
     1621 aaaacgtttc ataacacgac gctcggcgag ctctggagcg acgtggccag cgagcagctg
     1681 gagcacgata ttctcgttaa tcgccgggaa aaatatgcca gccaggttcc tgatggtgtt
     1741 gtttatctaa ccggcggcat cgactctcag acgtccgggc gctacgagtg ttacgtgtgg
     1801 ggctggggag cagaggaaga gtgctggctg attgataaaa caatcgtcct cggtcgctac
     1861 gacgaggagg acacgctgca gcgcgtcgac ggagtgattc gcaaacaata ccggcgcagc
     1921 gacgggacca caatcggcgt cagtcgctgg gcgtgggata ccggtggtat agatgcgcag
     1981 gtcgtttata accgctcgct gaaactcggt ccgctgtggg tcattccaat taaaggtgcg
     2041 agctcatacg gtcagccagt cgtaaatatg ccgcgtacac gtaacgcgaa taaagtctat
     2101 ttgtcgttaa tcggtactga tacggcaaaa gatttgctcg caatgcgcct gccgctggaa
     2161 cccgattcta aatcggcgac accaagtgcg attcattttc ccaacgacga cgaaatattc
     2221 ggcacgacag aggcaaaaca gctcgtctct gaagttctga tcccgaaact gattaacggt
     2281 cgcgtcgttt atcgctggga caaccagggc cggcgaaatg aggcgctcga ctgctgggta
     2341 tacgcgctgg cagcgctacg tatcagtaaa attcgtttcc agctcaatct cgagacgctc
     2401 gctgagcaac ggaaaaaatc acaaaacaaa ctgtctctcg aggagatggc cagaatgctc
     2461 ggagggagct catgatgtcg cgcgaggttt taacagaacg gctgctggaa gctgaaattg
     2521 ccctgcacaa attattaacg ggtagatcga ctgtatcgct gtctcacggc gattcagcgg
     2581 gaaataaccg gagctatcaa tactcacagg cgagtattga acagctccga acgtacatta
     2641 tcgagctgaa atcacagctc ggtctgagta cgggacgccg ccgtcccgtg ggagttcgat
     2701 tatgactgct cagcagttgc tcgggcctga cggtaaaacg ccactacgcc gttacgcggg
     2761 gtataacggc ggcggtcccg gcttcggtgg ccagctgatt gactggaatg caccacagca
     2821 aagcgccgat gcggcactgc tgcctaattt ttatcgcggt aacgcgcgag cagacgatct
     2881 cgttcgtaat aacggcgtcg cgtcgaacgc cgtgcagctg catcaggatc atatcgtcgg
     2941 caatctgttt aagttgagtt atcgccctaa ctggcgttac atcgggatat ctcgcgagga
     3001 cgccagggcg ctggcgcgtg acgttgaggt cgcgtggact gaatacgccg aggaccctca
     3061 ctgcacgatt gatatcgagc ggaaacggac gttcacaatg atgattcgcg aaggcgtggc
     3121 cactcacgcg ttcaacggtg aaacctgcgt acaaccggtg tgggagagct gcgccggcag
     3181 cgtttttcgg acgcgattca aaatggtctc accgaaacga attcgaaatc ctggctacgc
     3241 agctgacact caatttcgcc gcgccggtgt tgatatcgat aaaaacgggg ccgcggttgg
     3301 gtactggata gccgaggata cctatcccct cggcggagtt ggtaaatgcc ggcgcatacc
     3361 agcaaaactc agcagcggca gacacgcatt cattcacata ttcgagccgc tcgaggacgg
     3421 acaaacccgc ggagataaca ttttttacag cgtaatggag cggctgaaaa tgctcgatac
     3481 gctgcagcaa acacagctgc agagcgccat cgtgaaggcg atgtacgccg ccacaatcga
     3541 atcagagctc gacagccagc aggcgtttga atatatcgcc ggcgcgggta ctgatatcga
     3601 ctcgaacccg ctgagttcgt ttattcagag ttacgtgacg tactacaacg gcgcaaatat
     3661 caagctcggt ggtgtgaaag ttccccacct gcatcccggc gacaaactca gtttacagac
     3721 cgctcagaat gctgatgctg gtttcagttc gctggaaaaa tcactcctgc gttacgttgc
     3781 cgctggcgtc ggcgcgtcat atgaggaact gagtcgggac tacagccagg tcagttactc
     3841 cagcgcgcgg gccagcgcaa acgtgagctg gcgttttttc atgggccgtc gacgttttat
     3901 cgccgcacga caggcgtcac tgatgttctg ctgctggttt gaggaagctc tggcgcgcgg
     3961 agttatcacc ctgccacgct cagcggtccg gtcgttttat gaagcgcgga actcctggac
     4021 gaacgcgctc tggatcggcg ctggtcgcat ggcgattgat gggctgaaag aggttcagga
     4081 gagcgccatg cgcatcacga ctggcctcag cacgtatcag aacgaactcg cgctgcaggg
     4141 gcaggactac gaggaggtca tggaacaaca ggaatacgaa attcagcgcc ggcgcgaaat
     4201 ggggctgagc gaaccatcct ggtcagtatc tcacccctca aataccaacg ataacggctg
     4261 gggaggtaac tgatgccgtg gaacaatttt ccgcacctcg ccgctagggc gttcaatcaa
     4321 ccgcttttgc tggagcccgc ctacgcgcgg gtattttttt ctgcgctgag cgaccggttc
     4381 ggtaccgggc gactgattga tacagcgtca ggagaggtaa tgaacagcga cgaaatgaac
     4441 gcgctcgcga tggggtggga cagcagcgag cgaacacgcc agaaatcgta tcgcgtggag
     4501 cgtggtatag ccgttctgcc ggttaccggg acgctggttc ataaattggg ttatatcaat
     4561 ccggtcagcg ggatgagtgg ttacgacgga atcgcaaaac gcctgcagca ggcgatttct
     4621 gatcccgatg ttaaggggat cctgctggat attgattccc ctggcggtga ggtcgccggc
     4681 gcgtttgata ccgctgattt aatcgcccgg gcgcgagagc aaaaaccggt gtgggcgctg
     4741 gccagcgata cggcctgcag cgccgcatat ttgctggcgt cagcgtgttc gcgccggctg
     4801 ataacgcaga ccggcacggt tggttcaatc ggtgtcctga tggctcaccg ctgcgtcgaa
     4861 aaggcgctgg agattgccgg cgttgacgtg acgctgattt acgccggcgc gcacaaagtc
     4921 gacgggaacc cgtattccca gctgcccgac gacgttcgcg acgaattcca gctgagtatt
     4981 aacagcacac gcgagcagtt cgcgcaaaaa gtctcggatt ataccgggct gaaaaaatcc
     5041 agggtgctgg ccacagaggc cgcagtattt atcggcgcgg acgcgattaa atctggtctc
     5101 gctgatcaac tcgttaatta cgcggacgct atcgcagtga tggccgacgc actgaaacca
     5161 aaaacggagc gatttatgcc aggtacaaca gaaaccacgg cggagaccac gaccacagaa
     5221 caaaccgcgg ctacgactac ggtcgcgccg gttgagtcca acgcggagca gattcgcgcg
     5281 gacgccgcat cgagcgaact ggcacgcgtg atggccatca tcaactgtcc cgaagctgtt
     5341 gggcgcgagg cgcaggcaaa agcgctcgct gccgtccccg ggatgacggt cgggcaggcg
     5401 caggctgtcc tcgcggcagc accgcaaaca gcgcaggcgc ggacagaaac ggcgctcgat
     5461 acactcatga gcactgaatc accggaaact attcaggatg ccggcagcac cacggcaaca
     5521 ggaacaaccg caaacgtctc gatgctggtc gcggcagggc gttcaatttt aggggatgaa
     5581 taatgaccac agaaacgtat agcccggatg attttattct gggacccgat cttgttgtga
     5641 cgaccgtcgg tcattttcag ggcgggatca acgtaccccg gctgacaccg attatgctcg
     5701 acgctactgc cgggacgttt aaagtctggg acggctcaat tggtaaggcg gttggtctca
     5761 cagccacagc agttaataca ggctccagtt ctgcagactg ctcgtactac aaatcaggat
     5821 cgtttcgtta tacggcgatt aactggggta cggttactga cgtcgctaaa cgcaaatcag
     5881 cgtttgctgg tacgccagtc agcgttggct gatagcaaaa aaaaactcaa aacaagccgc
     5941 ctccgggcgg tttttttata caggatatat aaatgagcga ttcgtttact acgtcagaac
     6001 tgattaccgc aacgcagcag gtatttaagt tcaatccgtt gtttttaaaa ttgtttttcc
     6061 gtgagaccta cacgtttacg agcgaagagg tttttctgga taaaatcccg ggcaaagtca
     6121 atatggcggt atattgcgcc ccgatgatca ccggcaaagt tgaccgcact cgcggctatt
     6181 caacgaacca ctttaaaccg ggttacacga aaccgaaaca cacgatcaat ccgaatatga
     6241 gcatcaagcg cgccgccggt gagcaaattg gccagccaga aacgccggtc gaacgtcgtg
     6301 caaaaatcat catgcagaat ttgctcgacg aggagctcag catcagccag ctcgaagagt
     6361 tccaggcagt gcaggcggtt ctgtacggta aatacaccgt ttccggcagc aatatcgaga
     6421 cctatgagat cgacatgagc cgcagcgcga cgaataacgt cactcagtcc ggttcgaccg
     6481 cctggtcgac tcaggacgcg gaaacgtatg acccgagcga cgatattgaa tcctatgcag
     6541 acctcgcctc cggtgcggtt aacgtgatca tcatggacgg taaagcctgg aagcagctta
     6601 aacgctttaa aaaattctgg acggcactgg atacgcgccg cggctcaaac agtcagctcg
     6661 aagtcgcgct gaaaaacctg ggcgatgtcg ttagctttaa gggctattac ggcgacacgg
     6721 cgctgttcgt ttacaagggt caatatattg acccgctaat aggcactgaa acgcgttata
     6781 tgccggataa cacgatgatc ctgggcaaca caaaaaatcg cgggctccgc acctatggcg
     6841 cgatccagga cgaggacgcg ctgaaagagg gtatctgcga agccacgcgc tatccaaaag
     6901 tgtggacgac gaccggcgat ccggcagtga cgcaaacaat gacgcaatcc gcgccagcaa
     6961 tggtcctcac cgacgctgat gcgttcgtcg tcgtaaaaat cgcgtaagag ccgaaggctc
     7021 ttttttttaa ggaattaaca tgagcacaaa aacagaattg ctggcgcgca ttgacgattt
     7081 gagcgcccag ctcggtcgcg aattaccgcg cagcgggact attgctgaac tggaatcaat
     7141 cgtcgccggc gctgagtcag agctcgatat tctgaacgag caatctggtg acgcgggagg
     7201 catcgagatc acggtgaatg ctgcgcccgg gacggatagc gatctcgctc aatcaatcgc
     7261 gagcgctatc gtttcacagt cacaagaacc ggagctcgca ccggctacgc accgcgttaa
     7321 gctgcgaaac acgctggacg tttatcacta cgtgaacggg cgtcgcgttc gcgagattgt
     7381 tgctgctggc cgggaaattg ttgttgattc accggaggtt gcagacctca tcgcagctga
     7441 tcacgtttac gcgctatgag ctactacgat gacctccggg ctggcgacga ggagatgatc
     7501 cgtgagtggg gacggcccgt taaattacgc ggtaaaaccg accccattat cgctattttc
     7561 aacgagcctt acgcgcgcgt tgacgttcct cacgctggtt ttattaccgg tacagtaacg
     7621 agcctgacag cgctctcaga cgacgtcgct ggcgttgttg cgcgtgacgt tgtccaggtc
     7681 ccaaagcagc gcagtatcgc tgctgacggc tctgttacct ggtctgactg gaccgattac
     7741 gtggttaaag agcctcaacc ggatggcatc ggtctcacga atattttttt agagcctcac
     7801 acgtccagcg aaaacagcga gtattcaaaa tactaagtgg gaccgcggtc ccacttaagg
     7861 gggaaccgtg gccgatttac gtagtaacgc gcagatgttc gatatcgatg tttcggcgct
     7921 ggagcagctc agagtcgaaa tcagcgcgac gcaacatcag atgctgatgg catacaacag
     7981 agcgctgaac cgaactgcaa agtatatgca ccgaatttca gcaggaatga ttttgactgc
     8041 actggcagct aaaaatcata aggccgtaaa taaacgaatc aaaccgttca ttaagcgccg
     8101 caattttaca aaagaagggg caggagatct gagtagcgta aaactctggt acggcctgaa
     8161 cgatttccgt gtatctgagc taaaggggcg attacaaaat cccaggaagc agaagcagcc
     8221 tcgcaatcca gagaccggtc aatttttgaa aacaaaaaaa ggcgctcgag gggcggcttt
     8281 tacacccaaa agtgcagggc tggcgatgat gagttggcca gattcattcg tagctaagcg
     8341 ctacggtgcg aaaagtgtct ggattcggct ggctcgcgga ggaatcgaag aggctcgcgt
     8401 acctgtgcac gacgcgctgg aggatgctat cgatgattat atttttgaaa acattggctc
     8461 tgtttttatg ggcttttttg agaaagattt acgcggtcga gtgaaaggta atgtccacgt
     8521 agacccaaaa acagggaaac gattatgagc gggctggatg catttgacga atatcttgat
     8581 cgcgttaaag gcgcggtttt acaaataccg ttcatcaaaa cattcggcat ttatccagaa
     8641 attccggcag gatttgaaac gccagctctg ttcctggaaa tcagcaactg gtcacagagt
     8701 gacgaagctg ttccaggctc aattcagtca gtcgagctgt cgtgtaattt gtatttgctg
     8761 cgtgagtttg ctgcagatca gtacggactg aaatcgcaga atgcagcgct ctatatgacg
     8821 agctggattg atgggcgaat gttcggtccc ggaactaaac cggcaaaatt cagcgacgcg
     8881 gaaccatgtg actggattaa aaacgggcag tcagtcggtt cgcattcagt tcagtgcgtg
     8941 tcgttcacgc aggttgtagg cgttggtcca gatattttcg attatccctc acagggaacc
     9001 ctgaaaaata tatacgtcgg aatagcgcca gatatcggcg cagaacacga gagcgactac
     9061 tatggcccaa tcggacgatg aatatgctgc agcggagaac gcgcgcaggc tgcgtgacgc
     9121 agttaaacga ggcacgatag ctgcagttaa aatgaatcct ccccgctgcc gagtctcatt
     9181 tggtggcgaa caccagtcag gctggctgca gtggtttact cacgccacct cagaacgcgt
     9241 cgactggagc gcaccatcag tgggcgatcc cgttactgtt gtttctgagg gtggggacac
     9301 gcggaacggc gtagttatgc tcgggctgca cattgacaac aaagctccgc ccagtaatga
     9361 cccccatgat catgtcactg catactgtga cggggctacg atgacgtata acacaaaaaa
     9421 tcacactctg acatggcagg gcgtaccgga cggcgtagta aaaatactcg gtgagtctga
     9481 aatagaaata ttcggacgtg cagacgttac tattaatagc gaaaacgttg tcaatattca
     9541 cggtggaaaa ttaattaacg cagacgctga cattattaat gtgacagcaa cagacacaat
     9601 taacgcacat gctgatttag tgaacgttat agcaacgagt tctgttagtg ttactgctgc
     9661 gaacagaata tcgctgacag ctcaaacaat cagtgcgtgg gctccgggtg ggataacact
     9721 agctggtcca actcatatta ccgagacatt agtagtagat aaattagcga cattccgtaa
     9781 cgatatttct gtcactggag ataacggtgg aacaggtaat atcacaaccc gcggtagtgt
     9841 gttagcaggg caagaggtac gggaccgaca aggcacaatg aatgagatgc gtattacata
     9901 taacgggcat acgcacatat gccctgacgg agaaacagag aaaccaaatc aaccaatggc
     9961 gtaaatatgc ttggaatgaa ccgtgacacc ggtaagcctt tatcggggac tgatcacatt
    10021 tgtcagtcta tcgttgatat tttaacgacc ccgctgggaa cccgtgtaat gctgccggaa
    10081 tatggaagca aattatttga ccttgttgat aatcccacag atccatcgct tgctatgcgg
    10141 ataattatgg aaagcgctgg cgcaatagca cgctgggaac cacgcgtcag aattgacaga
    10201 ataaatgtgt tagcagtgga tatcgggaaa ataacaatat taattatcgc aacagatatc
    10261 gaaacacaac agcgattaga gtttaataat atggagctga tattttgata acatcaacag
    10321 ttcaaaactc gatagtgaaa actattgata tgagtctgct tccgccgcca gcattcgtta
    10381 aaaccccgtt attttcagat gttaaatcta atctactgtc agagctgcag atattatatc
    10441 cacaatttaa cgcgctcctg gagtcagacc cagcagttaa actgctggaa atagttgcat
    10501 acagagaaat tatcattaca gcccgggtaa atcaggggat gctcgctgta ttgctcgcat
    10561 tcgcaaaaaa tagcgatctc gaccagattg gcgctaattt tgactgcctg cggctgttga
    10621 taacgccagc taatcccgac tcagtccccc cgactgaagc ggtttacgag agtgacgacg
    10681 agtatcgtca tcgcatacaa ctatcatggt acgcgcggaa tactgccggc agtacaaacg
    10741 cgtataacta tttcgcgcta tcgagtgatc ccgatgttct gtcagcgcag gcatacggcc
    10801 cgcctgtgac tcagccaggg tacgtcgata tgtacgtttt gtcccggaca ggcgacggtg
    10861 tacccccgca atcactactg aatacagtga atgcggccct gtctcctgac gacactcggc
    10921 cactaactga tttcgtgacc gtaaaacctg cgtcgaacct gaattaccgg gttgaggccg
    10981 ttatcgtagc ggggctcggt cccgaccaga acgtattact caacggcgcg cagagcgatc
    11041 ttgaagtcta cgtgaccaca caacataaaa tcggggcaac agcagctctg tcaggaattt
    11101 acgacgcgat acatcgcgac ggcactgagc gtgtgattct gatatcaccg acagaggatg
    11161 cgatcgccgg cgtcggacag gctccacact gcacagaaat caaactcagc gtacagatgg
    11221 ggtgacaatg acgagccaga gtgtactgcc tcctaacgca gtaacaccag aaagagcgct
    11281 ggaagccgtt ttatctcatg tcggcgattt gcctggtgat atacgaatta ttaaaaatcc
    11341 cgacctgtgt cccgcaaatt tgctcccatg gctagcgtgg gaatacgcgg ttacgtactg
    11401 gaatccggac tggagtgagc gacaaaaacg cgaaattatc aaagcagccg cgtggcaaaa
    11461 caaacaccgc ggcacccgtg gagctgttga acgcgcgtta ttaactgtcg gatttaatag
    11521 caaaatgcgc gagtggtttg aagccacacc cagggacgac ccgtatacat tcgcaattaa
    11581 aatatatctg ct
//
LOCUS       DAZUCE010000158        11543 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZUCE010000158 DAZUCE010000000
VERSION     DAZUCE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.158"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            169..1833
                     /gene="tsr"
                     /locus_tag="ACHY03_003577"
     CDS             169..1833
                     /gene="tsr"
                     /locus_tag="ACHY03_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFS3906764.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(1882..3243)
                     /locus_tag="ACHY03_003578"
     CDS             complement(1882..3243)
                     /locus_tag="ACHY03_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3906765.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(3458..4372)
                     /locus_tag="ACHY03_003579"
     CDS             complement(3458..4372)
                     /locus_tag="ACHY03_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFS3906766.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRSHLNSAKQSMIRSINENTRYAH"
     gene            4511..5533
                     /gene="lgoD"
                     /locus_tag="ACHY03_003580"
     CDS             4511..5533
                     /gene="lgoD"
                     /locus_tag="ACHY03_003580"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFS3906767.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            complement(5670..7961)
                     /gene="opgB"
                     /locus_tag="ACHY03_003581"
     CDS             complement(5670..7961)
                     /gene="opgB"
                     /locus_tag="ACHY03_003581"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFS3906768.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNAAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPKLA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(8215..8709)
                     /gene="yjjA"
                     /locus_tag="ACHY03_003582"
     CDS             complement(8215..8709)
                     /gene="yjjA"
                     /locus_tag="ACHY03_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFS3906769.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            complement(8758..9495)
                     /gene="dnaC"
                     /locus_tag="ACHY03_003583"
     CDS             complement(8758..9495)
                     /gene="dnaC"
                     /locus_tag="ACHY03_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFS3906770.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            complement(9498..10037)
                     /gene="dnaT"
                     /locus_tag="ACHY03_003584"
     CDS             complement(9498..10037)
                     /gene="dnaT"
                     /locus_tag="ACHY03_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFS3906771.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            complement(10145..10618)
                     /locus_tag="ACHY03_003585"
     CDS             complement(10145..10618)
                     /locus_tag="ACHY03_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFS3906772.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            complement(10609..11379)
                     /locus_tag="ACHY03_003586"
     CDS             complement(10609..11379)
                     /locus_tag="ACHY03_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFS3906773.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLSNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
BASE COUNT         2889 a         2808 c         3059 g         2787 t
ORIGIN      
        1 ttcgtgtgaa aaaaataaga aaagaaaatg tatcaataag tggttattgt atttttgaac
       61 aaaaattaaa aaaaagtgag gtaaaattaa ccagtttcaa gtcgctacct ataaagtttt
      121 ctcatttcag gccgaaaatc ttgcatcggt ccacaggaaa gagaaaccat gttaaaacgt
      181 atcaagatcg tgaccagctt actgctggtt ttggccgttt ttggcctttt acaactgaca
      241 tcaggcggtc tgttctttaa tgccttaaag aatgacaaag aaaatttcac tgttttacaa
      301 accattcgtc agcagcaatc cacgctgaat ggcagctggg tcgcgttgtt gcagacgcgt
      361 aacaccctca accgcgcggg tatccgctac atgatggatc agaataatat tggtagcggt
      421 tcaaccgttg ctgagctgat gcagagtgcc agtatttcgc tgaaacaggc ggaaaaaaac
      481 tgggcggatt acgaagcgtt gccgcgtgac ccgcgtcaga gcaccgccgc agcggcagag
      541 atcaaacgta attacgatat ttatcacaat gcgctggcgg agctgatcca actgttaggt
      601 gcaggcaaaa tcaacgagtt ctttgatcag ccgacccagg gatatcagga cggtttcgag
      661 aagcagtatg tggcttacat ggagcaaaac gatcggctct atgatatcgc cgtcagcgat
      721 aacaatgcct cctacagcca ggcgatgtgg attctggtgg gcgtgatgat cgtcgtactg
      781 gcggtcatct tcgccgtctg gttcggtatt aaagcctcgc tggtagcgcc aatgaatcgc
      841 ctgattgaca gcattcgtca tattgcaggc ggcgatctgg tgaaaccgat tgaggtggat
      901 ggctctaatg agatggggca actggcagag agtttgcgcc atatgcaggg agaactgatg
      961 cgtaccgtcg gtgatgtgcg caacggggcc aatgccatct atagcggtgc cagcgaaatc
     1021 gctaccggca ataatgatct ctcttcgcgc accgagcaac aggccgcttc gctggaagag
     1081 acggcagcca gcatggagca actgaccgca acggtgaaac agaacgccga gaatgcgcgc
     1141 caggccagcc acctggcgtt aagtgcttct gaaacggcgc aacgcggcgg caaagtggta
     1201 gataacgtgg tgcagactat gcgtgatatc tccaccagtt cgcagaaaat cgccgatatt
     1261 atcagcgtaa ttgacggcat tgccttccag accaatattc tggctttgaa cgcggcggtt
     1321 gaagcagcgc gtgcgggtga gcaaggacgt ggttttgcgg tggttgcggg agaagtgcgt
     1381 aatctggccc agcgtagcgc ccaggcggct cgtgaaatta aaagcctgat tgaagactcg
     1441 gtggggaaag tggatgttgg ctctacgctg gtcgaaagcg ccggggaaac aatggcggag
     1501 attgtcagtg ctgtgacccg cgtgacggac attatgggcg aaatagcttc tgcttctgat
     1561 gagcagagcc gtggtatcga tcaggttggc ttagcggttg ctgagatgga ccgggtaact
     1621 caacagaacg ctgcgctggt ggaagagtct gccgctgccg ccgccgcgct ggaagagcag
     1681 gccagtcgcc tgaccgaagc tgtggcagtg ttccggattc agcaacagca gcaacagcag
     1741 cgtgaaacat cggctgtggt aaaaaccgtg acgccagcta cgccgcgtaa aatggcagtg
     1801 gcagatagcg gggagaactg ggaaacgttt taatcgccat aaaaatgccc gataagcaaa
     1861 atgttatcgg gcatagggag tttaatcttt acgcgggtcg ttgatcggct gacgaaccag
     1921 gaagatgtac gccagtgctc ccgctgcggt gacgcaaccg caaatgatta gtgccagacg
     1981 gaatgagtgg gttgtatcaa caataaaacc agtaatgatc ggcgcgaaag aggcgcagat
     2041 gaagctggca aagttctgga tactgcccac cgacgcagtc atgcgagaag caactgcgac
     2101 gtggatcaag ccccagcagg atgttccggc aaagtgaata cagaacagtg ccatgccaat
     2161 cagcagaacc gccatcatgg atgttgtcgc ttgtggtact atcagcgtaa aggcggcaga
     2221 acagaacatc ccggcaataa tgcagatctt acggctttta atcggagcca ttcccccttt
     2281 gaccagccag tcagtaacgt aaccgttgac cagcatcccg gcagccccaa acaggaaagg
     2341 gatagccgcc atcaaccctg tgctttttaa atccaggtta taggctgttt gcaggtaacc
     2401 aggaagccag gccagataca gccacgcagt gtagttgatg ccactgaatc cgagcatcat
     2461 tccccacatt gtgcggttac ggaacaggct gcgccattcg gcaaaactga gcggatctcg
     2521 gcgggcattg acgctacctg cattgagata agcttgttca acggcagtca gttctacgtg
     2581 ctcgcggttg cgatagagca tataccagcc gatggcgaga aaaatcccca gtacaccaat
     2641 ggtaataaac atcccgcgcc agcccatcac cagcatcatc gccgccagaa tcggtgggct
     2701 tacggcaacg ccaatggtag aagctgcgtt gaagaagccc atcgggcgtc cgcgctcttt
     2761 gatgttgaac cagtcgttaa tgacttttac accgcatggg ttcatcggcg cttcaccaat
     2821 ccccataccg atacgcacca acacgaactg cgtaaagctg tgcaccatgc cagacatggc
     2881 ctggaacagt gaccagaaga acatccccag tcccagcatc aggcgtgggc ctttacgatc
     2941 caatagtggg ccgcaaggaa gttgcgcaat gccgtaagcg agtgaaaaca cggagagcaa
     3001 agcgccgatt tcggtggcac ttaatcccaa ttcttcacga atcgttaaat ttgctaccga
     3061 cagcgaactg cggtcgagat aattgattac cgccgcaaaa aataataaca acatggcggt
     3121 ggtttgaatg cgtttaattc gggtactgcg ttgaactaat ccatctggtg gcacgggaat
     3181 atctgccgac gaagatggag taaatgaaga acgcggatcg agggtgatat tttctttttc
     3241 cacgccggac tcctgtattt ataattttaa ttgtttacgt aattgtgcat tacgcaaggt
     3301 tgacattaac ggcaggttaa agttttagga ctttactacc gctcacaaaa acatttttag
     3361 tcgactgatt gtgagcatag gtaagcggga atttcccggt atttatcttt tgataaaacc
     3421 gtgcacccgc taatagtttg ctgtttttaa tccgtactta atgagcataa cgcgtgttct
     3481 cattaatgga tcgaatcatt gattgtttcg ctgaattcaa atggctacgg agcgcaaggg
     3541 tggcatcaag gtcactgcgg caaatcagag cgctgagaat ggtcatatgc tcatcgacgg
     3601 caatgatatt gcgctgttta agatcgcttt cgtcccattg atagtgaaaa tgaaagatga
     3661 ctgagatgat ctcaagcgat tgatcaaaaa agatattgtc ggcagctgaa agcagtaagg
     3721 agtgaaaatc tctgtcaagt tgcgagaaca tgcgaaagct attgccgatg ttgtcacgca
     3781 gtagccgatg acgttcgagc atggttttcg cctgcagcca gcggggatca tgatcaggca
     3841 ggttgagaaa gtgctgaagt gaatgtgttt cgagcatttc gcgtagctca aacagctgct
     3901 cagcgtagga ttgatcgaat tgcttcatgc tccactggcc gcgcttttcg ctttgaataa
     3961 gattataacg gccgaatttt aaaagatatt ctcttacaac caccgggctg acacccgcgg
     4021 cccgagccag ttgcagttca gaaaaagttt ccccagggcg taactggcgc tggtttatca
     4081 tcgtgaaaaa agcctgctca aacactttgt tttgttcact cattgaagcg gtagtacagg
     4141 cgaaaccgtc atcatggtca ggtttacgcg caataacata gtcgtttcca acctgcgtta
     4201 agacgccgca ttcgcgtaag tggctgagaa tatgacgcac tgtagtgcgg ctgatgttgt
     4261 acatttccgc cagcgcactt tgcgatggaa gtggggaagg gatatgaccc cgcgccatat
     4321 catcaatgac ctggttaatc acattgtggc gtaaattttg tgaacgactc atattttcct
     4381 ccttgtgagt ccattaaaac ccgatttaaa acatttttat gcgtgtgttt tcacaaaaca
     4441 gattttccgt tccgctgcca tttttcattt tcattgatat ctaaggcaac aaaaaatgga
     4501 gagccgaatc atgtctacga tgaatgtttt aatttgccag cagccgaaag aattagtctg
     4561 gaaacaacgt gagataccta ttccgggtga caatgaagca ttaataaaaa ttaagtctgt
     4621 cgggatttgc ggtaccgata ttcatgcctg gggtggaaat caaccatttt ttagttatcc
     4681 acgggtttta ggccatgaaa tatgtgggga gattgttggg ctgggtaaaa atattgctga
     4741 tcttaaaaat ggtcagcaag ttgctgtgat cccttatgtt gcctgtcagc aatgcccggc
     4801 gtgtaaaagc gggcgtacca attgctgtga aaaaatttca gtcattggcg tgcatcagga
     4861 tggcggtttt agtgagtatt tgagcgtgcc ggtggcgaac attttgcccg cagacggtat
     4921 tgacccgcag gcggccgcat tgattgaacc tttcgctatt agcgctcatg cggtgcgtcg
     4981 cgcagccatt gctcccggcg agcaggtgct ggtggtcggg gcggggccaa tcggtctggg
     5041 cgcggcggca atcgctaaag ccgatggcgc acaggtggtg gtggcggata ccagtccggc
     5101 gcgccgtgaa catgtggcaa cgcgtctgga gttgcccgtg ctggacccgt cagccgaaga
     5161 ttttgacgcg cagctgcggg cgcagtttgg tggttcgctg gcgcagaaag tgatcgacgc
     5221 gacaggtaat caacatgcga tgaataacac cgtgaatctg attcgtcacg gcggcacggt
     5281 ggtatttgtc ggtctgttta aaggtgagtt gcagttctcc gatccggaat tccataaaaa
     5341 agaaacgacg atgatgggca gccgcaacgc cacgccggaa gatttcgcta aagtcggtcg
     5401 actgatggcg gaagggaaaa tcactgccga catgatgtta acccatcgct atccgttcgc
     5461 cacgctggca gaaacctacg agcgcgatgt gattaacaat cgtgagttaa ttaaaggcgt
     5521 aattactttc tgataaggcg agattattaa agttgccatg cagcgtccgg ggaagtgttg
     5581 ggcgctgttt ttttgtttct taataatgtg ttgtaagccg gagaaggtgt gtaggtcgca
     5641 ccctatgcga cctacacatc agtcaggaat tacccttcac gttctaccac tttaatttcc
     5701 accatgccga tcccgagctt acgcggcgag tgtccgagga tattcccttc gttggttgag
     5761 acaggttccg gcggcacaat taccagtgtg tcggcatcgg ttgggttatc gaaatgcagt
     5821 gtggtggtgg tcacttcatt gcccagcaca agagtttgtt cttcattgcc tacgcgtacc
     5881 ggaataggac ggctggcgtt attgccgtat gctttggcgg taatcaccag gtcaaatttc
     5941 ttcggcagcg gatgcttgta ctcgattttt acttcatcgc ccagctgcgc gttggaccag
     6001 cggccccacg actccggacg ggaaatcccg ctaaactgtt tcacctcttc tggcgcaccg
     6061 gcgacgttaa agatgaaact gtcggctttg tagcgaatat cgttatcaac aatcttcagc
     6121 gtatcgacat tgcctttgta acgcgccatg tcgatgaccg tatctttaaa tgccgttttg
     6181 cccttccatg ttgttttgtc aacatgctga acaatttgct gaccgccaag ctgcccttgc
     6241 gagacacacc agtcggtgga gagtgccagt ttcggagccc agagttgtgc catcttatag
     6301 caacggtcaa cccagacgaa attgtcgcgt ggagcgaaat cggccagctg gaaacgcagc
     6361 ggtgctgagt attcgctttc tggcagcggt tccacgcgtt tgtctgaaac ccgcaacagc
     6421 agcggcaaac ggaaatggct acccgagaag gcaatcatgt ttttctgctg gtcgatggtg
     6481 aactctttca tctctttagg gaatttccac aggcggatga tatccggctt ccacgccaat
     6541 gttttctctt tgatattgag gaagatttcc gacattgact gcccggataa actgctacga
     6601 ccaagtccga gatagttatc gccaccgaga atgtccagca ccgtcgcgcc gttatccatc
     6661 gtattacgct tcactgccag cgtctcttgc tgcggcttgt cgccacgaat gacaaaaaac
     6721 aggttattgc gatcctgctt attgaggtat ttccacgccg tgttgttcat cgctaaatgg
     6781 tcagaagaga cgacgatgac ggtatcttta aaccacggtg acgctttgat tttgttgata
     6841 aacgccgcga tattctcctg gctgcaactt accgcgctga atgactggtt cggcttaccg
     6901 tcaaaatcat attttttgcg gttacaggta cgcgagataa aaccatccgg gtgatgggta
     6961 tcgactgtca gggtaaaaag tgagaatcgc tgacctgagc gggaaagctc ttcaaacttt
     7021 ttccacgctt catcgagaac ggtatcgtcg tagaatcccc agtcgttgcg atagtgcggg
     7081 tcggccacca cgcttttcag ctcttctgag ccgtataagt ggtcgaagcc atgcgacttc
     7141 aggaacacat ctttaccggc aaaacgcaga tttgcgccct gcacgaaata gttctgataa
     7201 cccgagtttt tcaggatatc tcccagacag atgttctgcg ggaagaagct ggagacagag
     7261 gcggaggcgt tgccttcaaa gggggcaaac agcggtatgc cgcactgaga agccaccatg
     7321 cccgcaatcg tgtaatccgt tcctggcagc tgctgcgtgt ggctgaaatc caggccttca
     7381 tttttcaacg cgcccagttc aggcgtgaga tccgggaaag ctgcgttatc aaaataggtc
     7441 cgctcgagac tttcgccgta gatataaacc aggttgagtt tcgggtcagg gatagttttc
     7501 gacggctctt tataataagc cgcaaagtcc gggtcgccgt cgcgtgactg ggatttcacc
     7561 agttccgtta tctgacgaaa tgccgggctg gcgtccaccg aacccagcgc cagtaagagc
     7621 gccagcaggc tgtaaccaaa atggtgcgga tgatgacgac gacggcgcag gatccagccc
     7681 agcgcaccga acaccgccgt cagccccagc acaatgccga tacccggcag gatgtatttg
     7741 ctgacgccag caccggtcag gctgttggtt aaggtataga gaaccgcgtc gttaatacca
     7801 tcgccagtaa aatagtcgct ggcaaacagg gtgatattta aaacgacaaa tagccccagc
     7861 accgttaatg tggctgcaaa ccaccaggtg ttacgtcccg ctttccatgc gtaaatcagc
     7921 acagaggcga gaaaaagggc gaaagagagt agttctgaca acgggcgatc ctcactaaac
     7981 cgggccgttt agccaacgtt taagcttttt gagaagcaca atgtaatggc gtcgccatat
     8041 tgctgcaatt ctagtgtcaa aaaaatgcga tgttgttagg aattagttta taaacagact
     8101 tttttgatcg cggtcgcttc ccgtgtaaca gggggaaaac cgtccaggag cagggaaaag
     8161 ggcaggagag agccgcgtca gccgggtaac agctaaaacg cggcgtttcg cggttcagga
     8221 aatgaagttc agcccctgct ttaacaccag atcgcaggct ttggttttca ccttttctgc
     8281 cagcggagtc gttccgatgt tatccagatt gagttgctga ccatctttgg ttttcagcaa
     8341 accctgaata ccgtccagat agttggtgtc ttctttttgc tcttcactgt tcaggcccag
     8401 cttttccagc acctgattct tgatgttttc ggcatcggtt accgacgcca gcttttgctt
     8461 cgcgcagtat tgcagaatgc ctgcggcatt gttcatgtta tctgcgctta aggcctggtt
     8521 tccgctgcta agcaagttag ttaatgacgc gagcgaccaa ccgccttcct gtgtcgtgct
     8581 gttttggttg ccaagttcgc tggcggcgct ggagagcgca tctttccagg acgcagcatg
     8641 caccccggtg gaaattaatg cgctggcggc aatggcacag cacagaagat gtttaacagt
     8701 tttcatcatc attactcaag gtggaattgt gtcgcagtat accgccagaa acggggatta
     8761 atactcttta cctgttacac ggctacggta gctatcccag ttgaagatca cccacaaact
     8821 gttacccagg cgcatacggt ccatcacgcg ttcgcccagc aacttggtca tctcttccat
     8881 attactgttg gtcagcatcc cggttgggcg tttggaagaa gagcggcgat cgacgatctg
     8941 gttgatgatc actttttcgt atttcgattc ggtctgcacg ccgatctcat cgatcaccag
     9001 cagatcaacg ttgctcagat cgttaagcag ttgttcttcg ctggtaccgc tattcctgaa
     9061 ggtatctttc atcgccgaca taatatcggc cacggtgatg atcaatacgg atttaccgcg
     9121 tagcagcagc tcgttgcaga ttgccgccgc cagatggttt ttgccggttc ctggcttacc
     9181 agaaaagatg aagctggcga tgttgccgtc aaactcttcg acatactggc gcgctttgct
     9241 taacgcattc atctgccctt cacactcaac gcgatagttc tcaaaggagc agttctgatg
     9301 cagtggacga ataccggagc ggttaaaggt gcgctgcatt ttcatcgccc gattttcacg
     9361 ttcgagagcg gcggagcgga tcgccccttg ttctttctgc caggccagta gctcttcacc
     9421 cgttttgaat gcaggtttga tatgggcagg catcattttt tgcaggcgtt gcatcaggtc
     9481 gccaacgttt ttcatcgtta ccctctgaat cctggtggaa tttggctgtc aggttcactg
     9541 accgtattca catctcgttt cggcagtccg ccgttgctgg cacgaccgat ttgcaggctg
     9601 cgcgccagtt tttgttgcca ctgcacatgg tgaaagactt taccttccgc ctgccagtag
     9661 gcaatgaatg aggccagttc ttcggtggtt accggctctc ttagcgccac gccccatagc
     9721 gccgccaggc ggataaaatc ggcatcgggt tgccagtccg gatacatggc gaatttcccc
     9781 atgggtacag caacgggagc ggcttgcggt tcctgataga gttgatcgtc cagagcgaca
     9841 tcgcttcccg gacgcgccag cttttcttcc agcgccagca gttcagccag gcgtgcaggc
     9901 gtgacggcat aaaacgccgg ggcattgtta gcaaatacgg caaccacacc gccttcagct
     9961 tttgccagaa cggtttggtg atcgtgtacc agggcgtcaa taccaacgac gtccggggtc
    10021 aaaactctgg aagacatact gtttctcaaa tatggaacga tgaacgagaa gggagataac
    10081 ttttatagta acacacggag gagaggaggc tgtctctcat ccgcgtgctt taagcgaggc
    10141 aattttatac gcgagggcgc ttgcggtaca accataatcc aggaatggaa agaccgatag
    10201 ataacgcacc aacaatcgat gaagctgtaa gaaagttggt taacagggta atcatcaacg
    10261 gttcgctgta acctagctgg ctgattttta ccgccgaaat cattgcggta taagccgata
    10321 tgcccgggaa catagggata acggccgcca cggtaaacac tttcggatgc gccagatacc
    10381 agcgcgacca ttgaatacca atggtgccga ccagcataga agccataaag gttgaccact
    10441 caatattcaa cccgctggtc atcaagatca ttcgggaacc atgacctatc gagccaagca
    10501 gcgcacacca gcgtaacgcc cgcacgggaa cgttgaacac catcgcaaag ccgaccgcag
    10561 gaatggcggc gaggatcata tcctgcgcca atgcaaacag aaattcgatc acacccatcc
    10621 gcgtagcccc caaatcgtca gtgccatcac cacgccgacg caggtagcca gtgtcagcag
    10681 actggcgatc gcccagcgtg ccagtccggt attgatgtgg cctttaaaca tatcggcgac
    10741 ggcattaatc aacggaaagc ccggcaccag cagcagaacg ctggcggcca tcgcaatggt
    10801 gggggtattg ctgaaagtgg ggagttgcaa aagcaatccg gaaatggtgg tggcggcgaa
    10861 agcggtaagg caaaagttga tctgtggatg aagatgacgt tgtgccagca gctggcggat
    10921 atacatcgcg gtcgtactgg caaagaaggt gatgacggca ccatcccagc caccgttact
    10981 gagtttacag aaacaggcgc aagaaaggcc aaccattaag gctaccagcc atcttgggta
    11041 acgtaatggc tgaatttggc taaatcgttt ctctacgcct ttgtaatcca gcagatgatg
    11101 ctccgcaaga atcacaatgt gctggacttc agtcacaaca tgcatattaa tgccgcgatc
    11161 gtgattttta cgtgtcgatg tcaggcattg cccatcttta atagttgtca gcactatggc
    11221 gttcgaagag atagaacttt cgacgctgtc cattcccagc gcccgaccca gtcgtgagga
    11281 aagctcatca accaacgcgc tttccgcacc atgttgcaaa agaaataatc cacactggat
    11341 acaaagccgc gttacggctc gctgttgctc agtttgcata actcgccctt tgtcatagac
    11401 gtgatgcgta ttgttcagat aatgagaatc catttgtatc atgaagcacg gttgttttgc
    11461 ggtaggttaa gatcaaaatt gcgctgatta atcctggtta tcgggagacg cttggtgata
    11521 aaaagcgaat tatctctcgc cgt
//
LOCUS       DAZUCE010000159        11529 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZUCE010000159 DAZUCE010000000
VERSION     DAZUCE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.159"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1886)
                     /locus_tag="ACHY03_003587"
     CDS             complement(<1..1886)
                     /locus_tag="ACHY03_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFS3906774.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEDSLSDTANALAENARNAGEL
                     GRFMSVVRVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEA
                     ELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLVHEKETLEY
                     KRQLAELGDKVEHQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEEQR
                     LRDV"
     gene            complement(1936..2238)
                     /locus_tag="ACHY03_003588"
                     /pseudo
     CDS             complement(1936..2238)
                     /locus_tag="ACHY03_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(2265..2687)
                     /locus_tag="ACHY03_003589"
     CDS             complement(2265..2687)
                     /locus_tag="ACHY03_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFS3906775.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTEHD
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            complement(2703..3452)
                     /locus_tag="ACHY03_003590"
     CDS             complement(2703..3452)
                     /locus_tag="ACHY03_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3906776.1"
                     /translation="MATPNPLEPVKGSGTTLWVYTGTGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SESRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDQGDITP
                     VTGITVTPPTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDGAAG"
     gene            complement(3460..3858)
                     /gene="gpU"
                     /locus_tag="ACHY03_003591"
     CDS             complement(3460..3858)
                     /gene="gpU"
                     /locus_tag="ACHY03_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFS3906777.1"
                     /translation="MRHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKITYEM"
     gene            complement(3871..4494)
                     /locus_tag="ACHY03_003592"
     CDS             complement(3871..4494)
                     /locus_tag="ACHY03_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3906778.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRAAATTLNRVAESIIAKTASSV
                     ARELAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVLLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(4497..4778)
                     /locus_tag="ACHY03_003593"
     CDS             complement(4497..4778)
                     /locus_tag="ACHY03_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFS3906779.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(4771..5097)
                     /locus_tag="ACHY03_003594"
     CDS             complement(4771..5097)
                     /locus_tag="ACHY03_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFS3906780.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWEDAPANSAAV
                     AVKINV"
     gene            complement(5185..7209)
                     /locus_tag="ACHY03_003595"
     CDS             complement(5185..7209)
                     /locus_tag="ACHY03_003595"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFS3906781.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQTGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITNINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKECSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(7154..8656)
                     /locus_tag="ACHY03_003596"
     CDS             complement(7154..8656)
                     /locus_tag="ACHY03_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS3906782.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(8656..8868)
                     /locus_tag="ACHY03_003597"
     CDS             complement(8656..8868)
                     /locus_tag="ACHY03_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906783.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(8865..10988)
                     /locus_tag="ACHY03_003598"
     CDS             complement(8865..10988)
                     /locus_tag="ACHY03_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFS3906784.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWALRETWLVFPQERSGETVSPELTTGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            complement(10985..11461)
                     /locus_tag="ACHY03_003599"
     CDS             complement(10985..11461)
                     /locus_tag="ACHY03_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFS3906785.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGLLVSVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         2422 a         3655 c         2746 g         2706 t
ORIGIN      
        1 tacacgtcac gaagacgctg ctcttcagac tcccgctgcg cctgacggtc agtgagacca
       61 cgggcttttg cgctgatggc agcctgcttc gcgctctgtt gctcttcaaa ccgcaccgcc
      121 tgctgtgcca gctcattcag gcgtttctgg tgctccacct tgtcacccag ctcagccagc
      181 tggcgtttgt actccagcgt ttctttctca tgaaccagca gggatttttc ctgcccggat
      241 aactgtcgtt tcgtggcagc ctctttcagg accgcatact gactttccgc cttccataaa
      301 tcacggcgct gccggctgat tttctcattc gcaccgctgt gcttctccag cgtcctgagc
      361 tcagcttcaa gggcaagcag agcctctctt gcctgctcct cttccctctc cccggcagag
      421 cgcgttttcg gtgatgtatg ctttttacct gtcagctctt cagccagacg gctgacggct
      481 tcctgctgcc ccggaccttt gctgacgcct gttgcacgcg aacggttgat gtaccccatt
      541 tccccctggc gtatacgcgc atcccgttcc gcaatggatt ttctcagcgc cagttcatcg
      601 cgttttgttt tctcaataaa tacgcggttc tcttctgcca gttcaccaaa caacgcacca
      661 acgccgggca cattctttgt cgtttcccag gctgactgaa taaattcagc cagcgccaga
      721 tccccctgca caagcagcag cttcacctgt tcaacggttc cggccaccac gtcagtgatc
      781 agactgagtg cccccagtgt atgatcacct atccatgccc atgcgtcaga agtccaggtt
      841 ttaacatcgt cccagatttt ttccaccggc gtggccgctt tatcaagttg ctccagacgt
      901 gcattcatga catccgcaaa cagggacatc gcctccgtca ccgcagcctg tttacctttc
      961 gtgcgctcaa gctcatcaat atggcgtaac tgggaaacgc tcaggaagtt atactgctga
     1021 ttcagggagg ccagcgcctt caccggatct gctgcaatcc cttcaaaggc tttttccacc
     1081 ttcccggcat cgtcccccac cgtctgcagc catctctgag aggtttcccc catgatccgt
     1141 agctgcccgg cggtatattt cccgctttct gccagacggg ccagattttc tgccgcctgt
     1201 ctgataccac caccggcttc atcgctgatc accccggcca ttttccacaa ttctgccgtt
     1261 gtggtggcag ccgcccctcc ggtcagggtc agtgaacgca ataaggcccg gtcagcctgc
     1321 tctgcctgcc aggcggcagc agcaagcgcg gccagtacgg caaccccgcc acctgccgcc
     1381 acacggacca ccgacataaa tcgtcccagt tcaccggcat tccgggcatt ttcagccagt
     1441 gcatttgccg tatctgacag cgaatcctct gatgattcag aggcatccct gatcccgaga
     1501 agttcctcct tcagcagggt aagcaggctg agcggtccac cgaatgaatc gctgatctgc
     1561 cccccctgct gcagcatgat aaggaaggga ttctgaccac cggcaagctg agtgacaata
     1621 tccgtgaact gtgcgggcag tgtgcgcatg gcagccttat actgtccgac tgatatcccg
     1681 gctttttgtg cagccagcgc ctgtcggctc agactctgct cagcagcatc agcctgtttt
     1741 ctgaaagact gactgacttt cccggacatc aaatcagcaa gcgcactggt ttcacccagc
     1801 tcttttttta cccgcgctgc ctcttcagaa aaacgggcag aatccagtgt cagcacggct
     1861 gtcagatcgg caaaattacc tgccatcgtg gtcacctcct gaaatgtcct ctgatgccat
     1921 caataactgt cacaacctcc ttccctcctc cccgaaacgg actccaccgg cgaggcccgc
     1981 cgccttctgc atcagtacat cattttcgtc cggcgtctcc gcctgccctt cctctgcctc
     2041 cggagcgaac aggctgaaat ccgccggatg catatccgga tcgcaaaaaa acaggctgag
     2101 tacggcgtac gtcagcccgg aaaaatgcat atccagctgg gtatcctgaa aataatgcgt
     2161 gcagtaaaaa cgtcgccagt cggcatattc ggtggatgtc atcccggcaa gcatggcgcg
     2221 ccagtcggct ctccccatct cacgcgccag tttcaggaca aagttcagct cgccttcgaa
     2281 tgcttttttg atgttaccgg ctcagtcgct tctgctttcc cggtttgttc aggatcggca
     2341 tcgtgccggt tatccagcat acctgaaaga taaagcaccc ggttcgttgc ctgattcagt
     2401 gcatcagcag gccatcccag catcacttca cggcggatct gctgcatctc tgtctccgga
     2461 gaggccagag tgcctttgag ggaatgggaa tgccatagcg acatcgccac aagcagggat
     2521 gccgtttcca gatatcgctg gttaatgtgc acggcatcat gctccgttgt ctcctgttgt
     2581 gctgcgtctg aaacaaactt catataatca aaccgctgca gcgcagacag ctccgacaac
     2641 gtgacggaca ccccgttata ttcaaattgt tctgttttca gaaacatgct ttatctcccc
     2701 cctcagcccg cagcgccatc cgtgacggtg atctccgcca ccgccgcaaa ctcaccattg
     2761 ccggtaacca cagggatctg cgctttacct gccgcaacac ctttcacggt gatcgtgttc
     2821 cctttcacgg taatggtggc gaaattctga ttcgccgacg tggcacggaa ggttttatcc
     2881 gtcgcccctt ccggctgaac agccacggtc agggtgatat tctgaccttt tgccacattg
     2941 cccgttggtg gcgtcacggt aataccggtg accggtgtga tgtccccctg atcttccgcc
     3001 agcgacggac gaccgatatt ggtgattttt accgtacggg tgatcacctc tttggcggtc
     3061 accgctttac caatggcgct cacccagcca cggaacacat ccaccgtacc gttcgggaag
     3121 cgaattttgt aggcccggct ctcactgctg tcaaaccagg caatcaaatc gcgctgccct
     3181 ttctcacccg gcttccaggc cagcgtaaaa ctggtgtcac cggcagattt ctgcccctgc
     3241 ccggtggata cccagtcagc atcctcatca tccagatagt tatcatcgta ggattctgcc
     3301 gtcatctcgc cgggggtcag atccttcacc ttcgccagtc gctgccagtc atcgtctgac
     3361 aacgggtttg cataagcatc accagtgccg gtgtacaccc acagtgtggt accggaacct
     3421 ttcaccggct caagaggatt tggtgttgcc atatcgtcct cacatctcgt aggtaatttt
     3481 ccacaggaga tctgccgatc cccacatcat aaactcatca tcccggcggt agtcataccc
     3541 ctgaagattc attttcagca gtaatgcact gagcccggga actgcctcca gcgcaggaag
     3601 aattttttct tccatccaca tatccagtgc cgagtccggt tcttttgccc tgagaaaaac
     3661 ctcaatatgc agtgtcgcct cccaggttcc ctcatcaacg aactcgtcag cagcagacgc
     3721 atctgtcagg taaacagcaa cagcaggcag ttcctgttca tcaataaaaa ccgggcgacc
     3781 gtcaaaccag ctcaccctct cagaaatatt ttctttcagg gcagacagaa ctgccgcccg
     3841 tatttcacgg tgtctcatac accctcccag tcattttctt ttcagcacca ggcgtaactg
     3901 atgcgtcatg gctttcatca tctgcgccgg taatttttcc cggtacatcc ggtcccgttc
     3961 acgttcaaag gtttctgcca gcggtccggc agtcggaatc ttcaccactt cgatcggcag
     4021 acggtggcgt ttcggcctcc ctttgctgtc agcgccggtg gacgatgatg cccacggcat
     4081 acgctgcatc acatgccagc gtccgttagc cagccgggtg ataaaggcat ccgggatccg
     4141 tcttttcccc acaatcagca cgctgccacc gcctttcgtg accgaacgct cgcctttctt
     4201 tcgtcgcttt ctgcgggaaa gcagcacgct ggccgtcccc agttttatgg cgggcagatt
     4261 accggtgttg atgatgacct tcgcataaac cctgtctgcg ctggcccgtt gcaggcggat
     4321 acgctcacgg atgagacggc gcggaaccgc cagctccctg gcaaccgaag aggccgtttt
     4381 cgcgatgatg gactccgcca cacggttaag tgtcgtggcg gcagcccggg gaacggcacg
     4441 gcggtcaatt gcatccagat ttttcatggc ctgcgccaga ccttttattg ccatactcat
     4501 tcctgttcga caaaaatccg gggtttaccg ttgtacgtgt cataacgggt caccgtcagt
     4561 gtgcgccccg caaacacaac aacatcatga cgggccggac ggtaccgggc tgaaaacacc
     4621 accagtgaca actggctgcc cgaaagcgcc cccatctccg cggactcttc ctccggcatc
     4681 acgtcgtaca cgacgccgtt aatctccgcc tgtttgccca tcacccgaac ggtcgccacg
     4741 tccatccggc aacacattcg cgtaaacaga tcagacattg atttttaccg ccacagcggc
     4801 gctgtttgca ggagcatctt cccaggctac tcccgctgcc accgcaccgt ctgcagccag
     4861 ctgcacaacc ccgtccttca gataaaccac cgcgccggac tgaatatcgt cagcagactg
     4921 tttgggcagc aggaacacgc cttcggcaaa accgtcaccg gcctcaccgg caggaatatc
     4981 ggtaatggcc acggccacca tactgccgac caccaccgca gcaccgctca ggatggtctg
     5041 atctccggca ttcaccagtt caatggtggt accgtcctgt acaaaatttt tcgccataat
     5101 gctgtttctc cggacagccc ctgtggggct gtttttcagg cataaaaaaa gccctttcgg
     5161 gcagtgattg tgataacgcg gttatcaggc caccgacgaa cgcaccagcc cgcgccagtc
     5221 aagtggcgcc actccggcat caatacggat ttttgtggca ataccgtcag tggtgaaacc
     5281 ttcctgctga tcaatgtatg gcgtgtccac accatccagc caggccactt caatggtgtc
     5341 agtgccctgt gccgccgcca gataccaggt tttcggatct gccgcatcaa gacgcgcttc
     5401 tgcaatcacc tcagcaaagt tctggatggg gttaatgaca ccggcgtttg catccgcccc
     5461 tttcacactg gccgatttga tggtctggtt cgccaccgtc tccagtgcca ccggtaccag
     5521 cataaaggcc ggacggatat tcagggcgcg atcgccttct ttctgcaggc gcatcatctg
     5581 acgggccgca tccagtccgg aaacagagat ccccccggtg gcaatatttt tgtgatcggc
     5641 atggaacagc gccttaccgt cggacagttt cgggttatcc gtcagcacct tgtaaaccag
     5701 gtcaccaatc gttgccttcg ccgcacgccc catcttcatc ggcacgtcca ccagcatatt
     5761 cagatcatca ttgataatgg cctggcgggt gatggagaaa atctccccgt aggtggccag
     5821 agcaatggtc tccttgcgat ctgaggtggt aatgtattta tactccgccc cctcacgaac
     5881 ctgacgcaga gaaccaaaac cgcccatccc cacgcgatac gctgttttga agtctgacag
     5941 gcgtccctta cgggtccact tctggaaggt ttcttctgat tcctcccagc cctggatcag
     6001 ccccttgttc gacacatcca gcaggatatt gccaaaatca gaggtgctgt gcgtcagcgc
     6061 cagcccgacc atctgcatgg ggttataact ggccacccca ataccgcgct ccgtcagtga
     6121 catacgagcc cattcacgca gggtcatccc gttataggcg ttatccttct cgacattttc
     6181 aaatccggca cgggccagca tcgcctggcg gatcccgtct cccacaaaat tgccgtttcc
     6241 ggcataaata tgggccggtg tgtttttgtt ggtcggcgag cactccttgc ccatttcatt
     6301 cagcagacgt tcacgggcca tttccagcga acagtcagga tcagccacgc actgtgcctg
     6361 aagcgtctga tagcgaccgc cgaacatggc aaacagatcg ttaatgcctg acatgcgtgc
     6421 tttctgctca gccataacgc gggcgcgaat ggtcgcctca tcagacactg ccggtaccgg
     6481 tgatggttct gttaccgccg gtgcagggat tgtcactgtg gtatcacgcg gggcactgtt
     6541 gcgtggcgga gtaatcatgt ttcggatgga ttccggcatc tttttaaatt cctctgtacg
     6601 ttttgactga atacatgcca ttgcctcaac agcgggtgtc acctggtcag caaatccgtg
     6661 tgccagacat tcggcaccgg acatccaggt ttcatccgcc agcatggcgg caatttcatc
     6721 ggtggttttt ccggttttct gcgcataggc tggcaacagt accgattcga ctttatccag
     6781 caaatcggca taactgcgca tatcctcagc atccccgcca ctgaatcccc atggcttatg
     6841 gatcatcatg aaggcatttt ccggcataat gaccgtatca ccggccatcg caatcacaga
     6901 tgccatcgag gcggcaacgc catccacata cacggtaatg gtcgccccct gatttttcag
     6961 ggcattaaaa atggcgatgc cttcaaagac atcgccaccc ggtgaattga tatggagatt
     7021 aatgttggtg atatcaccca gtgcattcag ttcgctgaca aactgcttcg cggtaactcc
     7081 ccagaaacca atctcgtcat aaatataaat atccgcgtca cccggccccc cagtctgcat
     7141 cctgaaccag gatttattct tcatgctggc tttcggtgtc gcgctgatac tgtcgttcag
     7201 ttccggcact gttgcctcct ttgtcgttga cggggtcagt atcaaagacc agccccagtc
     7261 tgctgttttc atcaatttca gccttgcggc gacgtttgac ctcatccgga ttgcgcccac
     7321 cggcacgcac ccagtcagat tctgtcgctg caccaccccg gatctgaatt ctccaggctt
     7381 cagcttcctt aaccgggtcg atccacggca tcaccggacc ggaatacgtc gcgttatata
     7441 gcgttttcat ctccacatcc gccggaattt tcagcagacc tgccgcaacc accatattca
     7501 gccatgtccg gtacaccggg cgggttaccg cgccaataaa acagtcctgc aggatcaggt
     7561 aaccatccgt ggactcgacc agctcctgcc gctgggcgct gtaggtgccg ttatagttac
     7621 gcgccgcact ggaaaaactc agacgactgc ctgctgccac tgcacgcaac tggccgttgc
     7681 ggaaagtttc aagattggga ttgggacggt cagatttgac catgccgata tcctcgccct
     7741 tgcgcaaatc gtcataaata atacccgggg tgatatggac ttcccgctcg gtttctttga
     7801 tccccggatc ttcatagtcc tgtccgtcac ctttacggat atacagtccc agcgccgcag
     7861 caatacgcgc cgctgtcagt tccgcatcct catactcctt aagggcactg atccgcatca
     7921 gcacccccga taacatggat gagcctcgcg tctgatgcag acgacgagtg aacttcaggt
     7981 ggatcatttt tccggcagcg atttctttcg tatcactctg ccgaccgctg accggataat
     8041 ttttataaac cagatatttt ttcggtcttc cccactcatc aagaaaaacc ccctgattca
     8101 gtccggcgga ttcatctgtg cgcatgggaa caaaatccgg ctccatcgcc tcaagccaga
     8161 atggcactcc cgccgtccgt tccagaccgt ttcccgcacc actgaccatc tgcgcaaaca
     8221 cttcaccatc ccgcagccag gtccgcagca gtaaacgttc aagcacagga cgggtatact
     8281 gccctgtcac atccggactc acggaccatt cagcccacaa acggcggata tccgcagcca
     8341 gctcagccgc catttccccg ttttttcgta atggctgagg ctccacaata attcccctgg
     8401 caccaatcac ccgctcttcc agcttgtcaa acacaccaat caccaggtca tgattgatat
     8461 caagaaaacg ggcctgctcc cgcagggaaa ccgcaccgta tttactgagc tgatcggcag
     8521 agcgattttc ccgccgggct ttatgtgtcc gggtcggttt caccgcctca taggccatga
     8581 ttaacgccct tgaacgcagt ctggctgctt tccacccggg ggaaaacacg cctatcacat
     8641 catcaataat tgccattaaa acctcgccag tttaaatcct ggttttcccc gcctgcggct
     8701 caccatcgcg gcaagcctgc gttcccactc ctgacgtccg gcgcggatct gagaaaggct
     8761 ttccagcgtc agttgctgcc cgttgaaggt gacagacttc ccctccagta cggccatttc
     8821 cgcttcacgg taccgctgta tcatttctct ggcttcttct gtgctcacaa ccagcctcct
     8881 gatgttatcc atggattatc ttccgcacgc tccgtccgca gttttttctt ccggcgacgg
     8941 cgtttttctg ccccggtcgt cagttccggg gataccgttt caccagaacg ctcctgcggg
     9001 aagacgagcc acgtttcccg cagtgcccag tccggtgcgg agggccagcg gatcttttcg
     9061 taaccatgca gaacggcaag cgcatccgca taaaccagca ggtcaaacgc ctcgttagcg
     9121 cccctgcccg gttttcgcca ttttccgtca ctgccgcgct cttcataggt cagctcatcg
     9181 taaaaccacc gccccagcca gtcgggaaag tggatatagt tcggccctgg tgtgtcacgc
     9241 cacagggcat tatttacacg gtccttaaac gcatccgtct gaaccagcca cagcgcgaca
     9301 tcgccactgg ctctggcacg gcgggcactt ctgccggtat tatccgggaa ggtacggtta
     9361 atcagcctgt cacggcgaag accatccccc ttgaacagaa acaccctgtt gcccagtccg
     9421 tcactccggc aacgacgcca gaaacgatag gcgttatctg tcaccccggc ttcccctccc
     9481 gtatccaccg ccatggccat cagacgcatg cgcacatccg gatcagaagc cagcggccat
     9541 gttttatgga acacatccgt cagcaacaaa tcccagtcct ccggatatgc cgccggatca
     9601 accggcagac tttcaccgtt gggactgcag cgcagtgaat gccggatgtt gtagcgatca
     9661 acaatccagc gttccccctg ctctccgtat ccggtgatct gcacaacaaa acggcgattt
     9721 ttaccgccct gtacgtcaac cgttgcctca ataaaacgca caccatccgg cacagatcgc
     9781 cggggaaacg gctcggcacg ctgttcaagc agttcacttt tacgctgttc cgtggctgaa
     9841 cggggcagat agggtcgtcc gatatcggtg ttcaccaccg ctttcagggt ctcttcactg
     9901 ccggttcgct catactcttc ttctgccgcc agcagtttaa aaatcagttg ttcccaggtc
     9961 tgaaacgccg cagccggccc ctccatccaa aatgacgcaa tccgggaatt tcgtggcgtt
    10021 ccggtgatac tgccgtccgc cgccgcccgt tcaccttcac gaagccagat cccctggtta
    10081 ttcagttcac gcttctgttc cggagcaatc aggccgcggc agtgcggaca catcaggcgg
    10141 gccgcctgcc cggcagccac aaaatcaggg ttattccggt aaccggtcat gttatccatc
    10201 accggctgaa aatattcccc gcagtgcgga cacggccagt accaccggcg gcggtctccc
    10261 cggttataca gtgacaggat ccccgttgtt ggcggtgcct catgtgcgcc gccacagcgc
    10321 catttggtgt cagtgatatc ccgcccgggc gaactttcga ccagggtcat ccccgaggac
    10381 ataaaggtgg tggtacgctt tgaggccagg gtgaaggcat ccccttcacc gtcgacgttc
    10441 tcaggaaaac ggtcataatc cgtcagcgcc acacgacggt aatccgaaga ggaaaataca
    10501 gtgatcgacg gccagccaat tttcaggaat gagccgtcaa gaaacatttt gtcgtggacg
    10561 ttgttgtcat tacgggaagg gctgaggcgc ttactgacct ccggactgtg gcgaaacgtc
    10621 ctggaaagac gcgttctgga atgctcacgc gcctttgtct cggtcatctg caccaccagc
    10681 atatccgccg gatcacagat gatgccgtac acaatccagc catcaatcag cccttcggtt
    10741 ttcccggttc gcgcaggtcc cacaaacacc accgcgtcat attcacgggc tgataatgta
    10801 ttaatggggt caatcatata gggcgtcagc gatgactccc acggaccgga agtattggct
    10861 ccccgtggta cccgcatata acgcctgatg gcttccgcta ctggtaaccg gccaggtggg
    10921 cgaaacagcg aggccacttc gcgccagata tcggatgcgc ggctatggct ctcgttcacc
    10981 tgattcacat atcggcctca tcacaacagt caatgactgc cttttccagt gtgtcgcgga
    11041 tctcatcaac cacaatctgt acttcattca gttgtgatgc ggtccacccc ctgtccctct
    11101 ccagccggtc aggccaggtt tccagtacct gaactatcgc tttcaccacg acagaaaagg
    11161 accgcctgac atcactgact gacacgagca gaccggtttc ctgctcaaat ttcagtcggt
    11221 cacgttctga ctggtaccat gctttacgcg catgaggatc catttcctcg ttatctacag
    11281 gcagaggagc tttcatcagc tcggcaagga tatctgtcag tcggtacagt ttgagattgc
    11341 tctcatgacc accggctggg cttatgtttt ttacccgagc cgcgacagtc tgtcgatgag
    11401 caccggataa tgcggccagt tgggaaatat tcagatgcag attttttaat tcacgatcca
    11461 taacttcccc tgaaaattat gtaaatacac gccagtgatg aacaaaaaac aatcaacttc
    11521 gacactaaa
//
LOCUS       DAZUCE010000160        11461 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZUCE010000160 DAZUCE010000000
VERSION     DAZUCE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.160"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            85..1698
                     /gene="mppA"
                     /locus_tag="ACHY03_003600"
     CDS             85..1698
                     /gene="mppA"
                     /locus_tag="ACHY03_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFS3906786.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYVVKH"
     gene            complement(1749..2780)
                     /gene="ynaI"
                     /locus_tag="ACHY03_003601"
     CDS             complement(1749..2780)
                     /gene="ynaI"
                     /locus_tag="ACHY03_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFS3906787.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            3024..3281
                     /locus_tag="ACHY03_003602"
     CDS             3024..3281
                     /locus_tag="ACHY03_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFS3906788.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(3331..4281)
                     /gene="uspE"
                     /locus_tag="ACHY03_003603"
     CDS             complement(3331..4281)
                     /gene="uspE"
                     /locus_tag="ACHY03_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFS3906789.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(4433..5185)
                     /gene="fnr"
                     /locus_tag="ACHY03_003604"
     CDS             complement(4433..5185)
                     /gene="fnr"
                     /locus_tag="ACHY03_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFS3906790.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(5380..5895)
                     /gene="ogt"
                     /locus_tag="ACHY03_003605"
     CDS             complement(5380..5895)
                     /gene="ogt"
                     /locus_tag="ACHY03_003605"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFS3906791.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(5906..7432)
                     /gene="abgT"
                     /locus_tag="ACHY03_003606"
     CDS             complement(5906..7432)
                     /gene="abgT"
                     /locus_tag="ACHY03_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFS3906792.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNAHYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVAVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(7469..8914)
                     /gene="abgB"
                     /locus_tag="ACHY03_003607"
     CDS             complement(7469..8914)
                     /gene="abgB"
                     /locus_tag="ACHY03_003607"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFS3906793.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(8914..10224)
                     /gene="abgA"
                     /locus_tag="ACHY03_003608"
     CDS             complement(8914..10224)
                     /gene="abgA"
                     /locus_tag="ACHY03_003608"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFS3906794.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGIIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10400..11308
                     /locus_tag="ACHY03_003609"
     CDS             10400..11308
                     /locus_tag="ACHY03_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3906795.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVREILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
BASE COUNT         3013 a         2797 c         2818 g         2833 t
ORIGIN      
        1 cttttttgat ggaagttgtt tgcatttctt taaggcgaaa caaataatta cgcatcaatt
       61 ttaatgtcgg ttagagggaa acttatgaag cactctgttt cagtcacgtg ttgtgcgctg
      121 ttggtcagca gcatttctct ttcgtatgct gcagaagttc cgagcggcac agtactggca
      181 gagaagcagg agctggtgcg ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa
      241 gccgtgggcc tgccagagat tcaggtcatt cgcgatctgt ttgaaggact ggtgaatcag
      301 aacgagaaag gggagattgt ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt
      361 atctggactt ttaccctgcg cgataacgca aaatgggcgg atggcacacc ggtaacggcg
      421 caagattttg tctacagctg gcagcgtctg gtggacccga aaacgctatc gccatttgca
      481 tggtttgccg cgctggcggg aatcaacaac gcacaggcga ttattgatgg taaagctacg
      541 cctgaccagc ttggcgtcac cgcagttgat gcccatactt tgaaaattca gcttgataaa
      601 ccgttgccgt ggtttgtgaa tttaaccgct aactttgcct tcttcccggt gcaaaaagcc
      661 aacgtagaaa gcggtaaaga gtggacgaaa cctggaaatc tgatcggcaa tggcgcttat
      721 gttcttaaag agcgcgtagt caatgaaaaa ctggtcgtgg taccgaatac ccattattgg
      781 gataacgcca aaacggtact gcaaaaagtg accttcctgc caattaatca ggaatccgca
      841 gccactaagc gttacctcgc gggggatatt gatatcaccg aatccttccc gaaaaatatg
      901 tatcagaagc tgttgaagga tattccgggg caggtttata cgccgccgca gctcgggacc
      961 tattattatg cgtttaacac gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca
     1021 ttaagtatga cgatagatcg ccgcctgatg accgaaaaag tattagggac gggcgaaaag
     1081 ccagcgtggc attttacacc agatgttacc gccggattta cgccggaacc ttcgccgttt
     1141 gaacaaatga gtcaggaaga actgaatgcg caggcaaaaa ctttgttgag cgcagctggt
     1201 tatggtccgc aaaaaccgct gaagctgacg cttttgtata acacttcaga aaaccatcaa
     1261 aaaattgcga ttgctgtagc atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg
     1321 caaaatcagg aatggaaaac ctatatcgat agccgtaaca ccggcaattt tgatgttatc
     1381 cgcgcctcat gggtggggga ttataatgaa ccctccactt tcctgacatt attaacgtca
     1441 acgcattcag gaaatatttc acgctttaac aatccggcat acgacaaagt tctggcgcag
     1501 gcatcgacgg aaaataccgt taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc
     1561 ctcatggagc aagcaccgat tgcaccaatt tatcaatata ccaatggacg attaatcaag
     1621 ccgtggctga aaggttatcc cattaataac ccggaagatg tggcgtacag tcggactatg
     1681 tatgttgtga agcattgatg tgatgggaac tggcgttacc cttgtggata acgccagtga
     1741 tgtcttattt agcgaccttg ttccggcggt gtaatgttat ccatatacag cgtctggctg
     1801 gggaaggcaa agtccgcgcc gtgtgactgt acaatatcga taatcttcaa ataaacgtct
     1861 tgctgtgcag caagccactc agcccatacc gtggttttgg taaagcaata aaccataata
     1921 ttcaatgacg agtcagcaaa ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg
     1981 gccgggtgat ttttcagcat ctcacgtacg gcttcgacaa taacgcccac ttttgccgca
     2041 tcctcataac gtaaaccaat ggtcgtggta atgcggcggt tggtcattcg tcctgggttt
     2101 tctacgctga tcgacgaaaa cagcgagttc ggtacgtaca atggacgatt atcaaaggtc
     2161 gtaattttgg taattcgcca gccaatttcc gctactgtac cttcgatatt tctgtccggt
     2221 gaacggatcc agtcgccaat actgaaagga cggtcgaaat agagcatgat cccggaaaag
     2281 aaattactca gaatatcttt accggccata ccgacagcca gaccaccaat accaccaaag
     2341 gtcagcaagc cagaaaggct catgccgaaa tgttcgccat aaagcagaac aagcaccaca
     2401 ataatggtga ttttgataat gcgcgacata atccgcgcac tggtgatatc gcgacctttt
     2461 ttaatctgct gtttttcaaa ctgattaatc agcaaaaata gcttaatcgt cagaataacc
     2521 gcaatcaggg acgtacagat aaaatcgata acgcctgggg tgataaattt gagtttatag
     2581 ttttctataa cataattaat aatgctacca acagcactga taattatggt gtagattaaa
     2641 aattgcaccg catggaataa aaaacctttt cttttacgat ttccgcggcg aaaccaaaag
     2701 ctcatcagaa tcaatgctgc gcagctaccg aaaataatga ccagattaag cgcattattt
     2761 gtaaacagtt cagcgatcat tgttttatca ggctcctcca gataattgtc gtcatgccgg
     2821 aaacccctgg cggggctatt ttaccgcgac aattcattca gatcatcaat agtcagggaa
     2881 ggaagtagca acattagcta actaatcaat gcgttgattg taaatccagc taagaggtga
     2941 ggttttcaga gcagacaacg gtgaaatgtc atggtattgt tacgtttagg taacaagaaa
     3001 tttgtctgca caaggattac atcatgatta tggcgaaact gaagtcagcg aaagggaaga
     3061 aatttctctt tggtttgttg gcggttttca ttattgcggc gtcggttgtg actcgcgcga
     3121 ctatcggcgg cgttatagaa cagtacaata ttccgctgtc tgagtggacg acatcaatgt
     3181 atgtgattca gtcatcgatg atttttgttt atagcctggt ctttactgtg ttgctggcaa
     3241 tcccgttggg aatttatttc cttggcggcg aagagcagta agtaaaaaat aggcccgata
     3301 actcgggcct tgtcagttat tgaagagtcg ttaatcgtct tcttcgtcat ccagttcaac
     3361 gggtgtctga tactggtcag gtttaatgac caacagatcg cagcgaagat gatcaatcac
     3421 ctgttccgcc gtgttgccga ggaatgctgc tgaaataccg gtgcgtccta ccgtgcccag
     3481 aaccacaatc cccgcctgta agtgctccgc caaatcagga atcacctctt ctggcagacc
     3541 tttttctacg tgcgtcatgt tttcattaat gccgaatttc tgccgcagag ctttcattgc
     3601 cagcaaatgt tgcccacgaa tggcatcgtt ataaacgctc ggatcaaatt ccggcagttc
     3661 aatcgcgata ttaattggcg ttactgggta agcgccaacc agatgaactt cggtatggtt
     3721 gacttgttct gccagttcga tcgtctcttt gaccagtttt tcattgagcg cattatgata
     3781 cggctcttca ctggcgagat tcaccgccac cagcgcctta cctccttccg gccacggctg
     3841 gtctttcacc atccacaccg ggcttgggca tttgcgtaac agatgccagt ccgttggcgt
     3901 aaaaatcacc gcttccagac ggtcatgttg gtgcgccatt tttagcacca aatcgtgccc
     3961 gccgctgatc acttcctgaa tgatggcttc gaaaggacgg ttatgccaga ccactttaat
     4021 ttcaatggga acgccagcat tgagataata ttttgcctgc tcgtggatcc aggctgtacg
     4081 ctggctaatg acgccctgac gcatagcggt acgttcgtcc ggggagagca gggtggtcat
     4141 ttcgtatgag aagtcataga tcggcaaaaa ggctttaatt ttgccaccaa tccgttgatg
     4201 taaataaaca gctcgccgca atgctggttg gtcgtcctgg ttaggatcga taacaacgag
     4261 catgttctga tacatagcca tacagggtct ccttacaaca actgtcaacg cagtttgtaa
     4321 ttaaaagatt aacccatatc tggtgaatga aacagtgatg aaccttctgc cagatcaata
     4381 aatcagaaaa atttaatgat atgacagaag gatagtgagt tatgcggaaa aatcaggcaa
     4441 cgttacgcgt atgaccagca agctgggcca gcgcatcgtt attttcgatg gtgatgtatt
     4501 tacctttgac tgccagcata ccgcttttct ggaagcgacc cagcagacgg ctgatggttt
     4561 ctaccgtcag gcccagatag ttaccgatat cgccacgagt catcgtcagg cggaattcac
     4621 gaggggagaa gccgcgttgg gcaaaacgac gggacaggtt gtagatgaat gcagccagac
     4681 gttcctcggc atttttcttc gacaacagca ggatcatgtc ctgatcgcct ttgatttcac
     4741 cgctcatcag acgcatcatt tgctgacgca gattcggcat tttaccggac aaatcgtcca
     4801 gcgtttcgaa cgggatttca cataccatcg aggtttccag cgcctgcgcg aagctcgggt
     4861 gatgaccgct gccgatggca tcaaatccca ccaggtcacc tgctaaatga aaaccagtga
     4921 tttgctcgtc gccttgctca gtgatggtat aacttttaat cgtaccggag cggatggcat
     4981 aaagcgattt aagttcatca ccagccttaa acagcgtctg gcctttctga ataggcttct
     5041 tccgctcaat gatattatca agctgatcaa gctcatgttc gttgagtgtg aacgggatgc
     5101 aaagctggct gatgctgcaa tcctggcaat ggatagcaca accgccagac tgaatgcgcc
     5161 gtataattcg cttttccggg atcataggtc tgctcaagcc gtaattgata tttgtcaatt
     5221 ttaacatctt tttagggagc aagtaagtct aagcaaacct taacagcaga gaattccgat
     5281 attagatgta aatatatgtc tatctatttg aaaaccctta agttgttaag ggtaacttta
     5341 cataaaagtg tgaacaagct ggcacaaatt gtttaatgtt tacagcaaaa gataaccttc
     5401 gtggcgcaat aaccactctt ttcgctgaac tccgcctgca tatccggtca tggtgccgtt
     5461 tcggccaata acccgatggc aaggtacgac gatgctgatg ggattcgatc cgtttgccgc
     5521 accaacggca cgcgccgcgc cagggcggcc caattgttca gccagttggc cgtaatgcat
     5581 tacctgcccg caggggattg tgcgtagtgt tttccagact tcacgctgaa atggcgtccc
     5641 ccctgtggca gtgggaagcg tatcaataat gctaagatta ccggcaaaat aatcacgaag
     5701 cttgtcgctt aaaccgcctg ggttggtggc agaaatgcgc tcatagcctt ctttgcgata
     5761 atggatgtcc agcagctgca ccatgcgttc gctgtactct tcccattcaa ccgcccgcag
     5821 acgaaattgc tcatcgcaaa tcacccacag tggacccagt ggcgtggcaa ttttttcttc
     5881 aagtaatctc agcatccgtt ctctcttaag acaaacgtgg gtaaataccc ggacctatcg
     5941 gcagaccgac aagataccac gccagcaaca ttagcagcca taccaccaaa aagataagcg
     6001 gatagggcaa gactaacgaa tagtaagtac ccagtttcgc gtctggtttg tagcgttgca
     6061 ggaatccaag aaacagtgga acaaaaggag ataccggcgc taaaggcaat acggatgagt
     6121 cggcaatacg aaagaggatt tgcgcaaatg ccgggtgaaa gccaagtagc ataaacattg
     6181 gtacgaaaat gggggccaga atcgaccaga ttgcggaacc gctggcgata aacatgcata
     6241 agaaagagga aagcaacgcc agaccgacaa acgccgggat gccgctaagc cctgaacttt
     6301 ctagtatatc ggtcagcccc acggcgatga atttccccat gttgctccag ttaaacatgg
     6361 cgacaaattg ggcgagggga aaaaccatca cgataaatcc cgccatctct ttcatcggtt
     6421 caatcattaa atgcggtaaa tccgcctgac gtcgaattgt gcgggtagcg atgccataag
     6481 ccagcgagac aacaaagaaa aaaagaatga tcagtggcac gataccttta ataaagggtg
     6541 atggcatcac ggtgtgatta atcggatcgc gcaatatccc gttttccggg atcaccatca
     6601 gcgcaatcgc agcaataaaa agtagcgata cgacacctgc tatgcgtaaa ccaaaacgct
     6661 gactttcggt caatgtctgc agtttctcat cgctgtttcc ctgccattga cctaaccgtg
     6721 gctcgatgat tttgtcggtt atcaggccgc caacaatcgt cagtacggct acggagctgg
     6781 ccataaaata ccagttatca attacactga cgtgcatttg cggattgaac gcagccgccg
     6841 cttccgtgct gatccccgac agcaacacgt cggttgtgac aatcagtaaa ttagccgtaa
     6901 agccgcaacc tacgcctgca atggcagcca gtaaacctgc aactggatgc ctgcccaccg
     6961 ccagaaaaat cagcgcaccc atcggtggca tgatcactaa cgccgcatcg gaagaaatgt
     7021 ggctgaaaaa agcaataaac agcaccatat aactggcgta gtgggcatta acatgcgatg
     7081 ccattttaac cattagtgct ggcagtaagc cgacgcgctc cgccagaccg gcacctaaaa
     7141 ccagcgccag gatcgcacca agtggagcaa aaccgctaaa gtttttaata acattgggta
     7201 aaaaccagtg taatccttcc acactgagca ggtttttcac caccaccggc gtaccatcgg
     7261 tcgggttttt cgcactgacg ccaaaggccg acaaaattgc cgtcgtcacc atgagtacga
     7321 ttatcaaata gataaagagc aaaaaaggat ggggaacctt gttaccaatt ctttcgaccc
     7381 agccatagag cttcccggat tgggaggacg acggtatgga tgacatactc atgggcattc
     7441 ctcggttgtt gtgtttgcgt tgttgttgtt attttaaagg tgacggtgtc acgtttttcg
     7501 ggatagggca gtgatacggt tgcgtgtccg taacttgctg atgctcttgt tggcattctt
     7561 gcaatagccc tgaatcaatg aagagattga ctgtggttgc tgccatagtt ttcgccgcca
     7621 gcagcattcc tttatgagca atagatgttc gcccctggct aaccagttgc cacgtatgta
     7681 gcggtgtacc gacggcaaaa caggggctga aacactgggc aacaggcagt ttccaactga
     7741 cgtcgccgac atcagtcgat gccgcaagca cgttatcggt ggcggcatat ggagcgacct
     7801 cattcgccag taccgtttca cgatgacgta gtgcaaaagc cttgccgttt tcgccaccgg
     7861 ttgcggcgat attattcaga ctgttttgcc gatcgttggg ggtgagcgta gcctgaattt
     7921 gtttcgcaaa agccagttct tcggagttcc attccggggt accaaaatgg gatagggcat
     7981 ggtacatggc attttctaag gtgcgattcg ggagataact ggaacaggct ttgtcgaagc
     8041 ggcattcaac cgtggtttcg gtcatcaatg ccgcaccttc ggcgattttg gcgacccgat
     8101 cataaatatg ctgcacgtcg gtcatttcgg gggcgcggat aagataaagc acttctgcct
     8161 gcgcctggac cacgttgggc gagatcccgc cgctatttgt gatggcatag tgtacgcgcg
     8221 ctttttcaat aatatgttcg ttgaggaagt tggtgccagt ggtcatcaac gttacggcat
     8281 caagggcgct gcgtcccaaa tgaggggaat tcgcggcatg tgctgcgatc cctttaaagc
     8341 gccatgatgc ctgaatgttt gccagcgtgc gggtattgaa cataccggca aaggcttccg
     8401 ggtgccaggt gagtgccgca tccacatcat caaatacccc ctcgcggacc atgaacgttt
     8461 tacccgagcc gccttcttcg ccaggacaac cataaaagcg cactgtgccg ccttgcccat
     8521 attgttccag ccatttcttg acggctattg cagcggcaaa ggcggcggtt cccagcaaat
     8581 tgtgtccgca accgtgacca ttttcaccgg gcgtcgcgga tgtaggttgc gcgcaaccag
     8641 cttgctgact taaacctgcc agggcgtcat attctcccag cagggcgata accggtttgc
     8701 cttgaccaaa cgaagcaata aaggcatttg ggatattgcc tacgttgcgg gtaacggtga
     8761 agcctgcaga ttccagcgcc gaagccagat gctccgctga ccagaactct tcaaaacgtg
     8821 tttctggatg atcccagatt tgatcggcaa tatcggtata acgttggcga tcggcttcaa
     8881 tcgcatcgtc gataaaacga tagatttcct gcatcagata cctcgcgtcc agggaaaatt
     8941 gagcgcggtg cgcgccagcg tttcgacggc aatagcgaga acctgctcgt caaaatcgaa
     9001 tttttcgttg tgatgacctg ccgccagctg tgtgccaaac accacgtagg aggcttgccc
     9061 ttgatgttgc tgcacgcggg ccatcattaa tgtggcatct tcggaacccg caggcgcttc
     9121 aacacgttca atggcctgat tgacccccgc gacctgagcc gcctgacttt gcaaccatgc
     9181 gacccattgc ggcgaaggag aactggcggt agctgcaccc atcagacgag tttcaacgcc
     9241 gacaccatac atggttgctg cgccctgaat cgcttgttgt gcacggtcaa aaacatattg
     9301 attaatgacg tcgctggccc cgcgtgtttc cactttcagc aacgccgagg caggaacaac
     9361 attacgaccg cttcctgcct gcataacgcc cacgtttact ctggaagcgc cttcgctgtg
     9421 cggggcgatt gcatgtagtg caagagttgc ttgtgctgcc gccaacaagg cattgtgacc
     9481 gtcttctggt tttgcgcctg cgtgagcggc ggtcccggtg aagtgcgcgt caaatttggt
     9541 ggttgccata aaattatcac tgccgcacac cacggtgccc gcaggtacgc cagtgccaat
     9601 gtgcacggca gtaaaataat caacatcatc cactacacct gcatcgacca tcgccctcgc
     9661 gccacgcgta ccttcttctg caggttgaaa aatcagtttg atgatgccat gtagtccgga
     9721 ctcgaattgt ttaagggtat gcgccagccc aagcccaatg gcggtatgcc catcatgacc
     9781 acaggcatgc atcattccgg cgttacatga cgcaaaaccg tcgcggtagg ggcgatggct
     9841 gacatcctgc tcttcactga gatccagcgc gtccatatcg acacggaaag ccatcaccgg
     9901 accgggtcga ccggtatcca gggtggcgac gatacccgtg aaaccacctt caaaagccgc
     9961 aatccattgt gctagcgcac cctgttgacg agcgcgctcg aattcgcgtt gtagagtgag
    10021 ttcatcaggt agtcccatcc ggctactttc attaactact tcgcggccca gcgctagtga
    10081 atagccgagc tgctgcaatt cttccgcaac aagggtggca gtgcggaatt ccacccagcc
    10141 agactctgca tagtgatgaa aatcacgtcg ccagtgcgat aattttgggg caagcgaatt
    10201 aacaaattga ttcaaagact ccataacctt tcccgtcatc agtaaaaagt gtgacccggt
    10261 tcacgtagcg atagttttta cttatcacta actgattttt cacagtttta accgttcata
    10321 aattaccctg acacaatcat ctgcattaaa gtagatgcca gtttctttgg tctgataaat
    10381 aacggttatc ggtggcgtca tggcttttca ggtaaaaatt catcaaattc gggcttttgt
    10441 tgaagtggct cgtcagggca gcattcgcgg agcgagccga atgttgaata tgtcgcaacc
    10501 ggcactgagt aaatctattc aggagctgga agaagggtta gcggcgcaac tcttttttcg
    10561 ccgtagtaaa ggcgtgacgt taactgatgc cggtgaaagt ttttatcagc acgccagtct
    10621 tattcttgaa gagctgcgcg cagcccaaga ggatattcgc caacgacaag ggcaactggc
    10681 agggcagatt aatatcggca tgggggccag tatttcccgc agtctgatgc cagctgtcat
    10741 atctcgtttt catcagcagc atccgcaggt aaaagtacgc attatggaag ggcaactggt
    10801 gtcgatgatt aatgaattgc gtcagggaga attggatttc accatcaata cctattatca
    10861 gggaccgtac gaccacgaat ttacttttga gaaattactg gaaaagcaat tcgcgatctt
    10921 ttgccgcccg ggacaccccg ccattggtgc ccgttcgatc aaacagttac tggattacag
    10981 ctggacaatg ccgacgccac acggcagcta ctacaaacag ttgagtgaat tgcttgacga
    11041 tcaggcgcaa acgccacagg tcggtgtagt ctgcgagacg ttctcagcct gtatcagtct
    11101 ggtggcaaaa agcgattttc tcagcatact gcctgaagaa atgggctgcg atcccttgca
    11161 cggacagggg ctggtgatgt tgccggttag agaaatttta ccgaaagcgg cctattattt
    11221 gattcagcgg cgtgatagtc gccagacacc actgaccgcg tcattaatca cgcaattccg
    11281 gcgagaatgc ggctatctgc aaagttaaaa ctgcataaaa aaatagagtc tgtcgacatc
    11341 cgccagactc tacagtacac acagcagtgc atccgcgtct taaatccggc attgtctcct
    11401 ctgcgccggt gactgtgtta taacggtttt aacctttagt tgccaatttt cttcagtgac
    11461 a
//
LOCUS       DAZUCE010000161        11442 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZUCE010000161 DAZUCE010000000
VERSION     DAZUCE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.161"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            242..541
                     /gene="yoaC"
                     /locus_tag="ACHY03_003610"
     CDS             242..541
                     /gene="yoaC"
                     /locus_tag="ACHY03_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFS3906796.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(661..840)
                     /gene="yoaH"
                     /locus_tag="ACHY03_003611"
     CDS             complement(661..840)
                     /gene="yoaH"
                     /locus_tag="ACHY03_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFS3906797.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            914..2275
                     /gene="pabB"
                     /locus_tag="ACHY03_003612"
     CDS             914..2275
                     /gene="pabB"
                     /locus_tag="ACHY03_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFS3906798.1"
                     /translation="MKTLSPVVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIFCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2279..2857
                     /gene="nudL"
                     /locus_tag="ACHY03_003613"
     CDS             2279..2857
                     /gene="nudL"
                     /locus_tag="ACHY03_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFS3906799.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3041..4405
                     /gene="sdaA"
                     /locus_tag="ACHY03_003614"
     CDS             3041..4405
                     /gene="sdaA"
                     /locus_tag="ACHY03_003614"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFS3906800.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSITR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGQ
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4536..6134
                     /gene="pdeD"
                     /locus_tag="ACHY03_003615"
     CDS             4536..6134
                     /gene="pdeD"
                     /locus_tag="ACHY03_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS3906801.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRVLIKGSPVLIQWTPAASSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6138..7694)
                     /gene="yoaE"
                     /locus_tag="ACHY03_003616"
     CDS             complement(6138..7694)
                     /gene="yoaE"
                     /locus_tag="ACHY03_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFS3906802.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7682..7840)
                     /gene="yoaL"
                     /locus_tag="ACHY03_003617"
     CDS             complement(7682..7840)
                     /gene="yoaL"
                     /locus_tag="ACHY03_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFS3906803.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8157..9128
                     /gene="manX"
                     /locus_tag="ACHY03_003618"
     CDS             8157..9128
                     /gene="manX"
                     /locus_tag="ACHY03_003618"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFS3906804.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9191..9991
                     /gene="manY"
                     /locus_tag="ACHY03_003619"
     CDS             9191..9991
                     /gene="manY"
                     /locus_tag="ACHY03_003619"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFS3906805.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10004..10855
                     /gene="manZ"
                     /locus_tag="ACHY03_003620"
     CDS             10004..10855
                     /gene="manZ"
                     /locus_tag="ACHY03_003620"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFS3906806.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10910..11368
                     /gene="yobD"
                     /locus_tag="ACHY03_003621"
     CDS             10910..11368
                     /gene="yobD"
                     /locus_tag="ACHY03_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFS3906807.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLERIYKLLVSTQ"
BASE COUNT         2806 a         2883 c         2869 g         2884 t
ORIGIN      
        1 ttcgcccttt gattagcaac tctggttttc tttcttagct gaacaatctg gttatagcgt
       61 gcggactgac cgttaatttt ctccatctta ctggataaca caccatgagc tgctaccttt
      121 aaaacacctg aatgtcaaca ggttaactcg tgccatatcg ttataatccc tttgatatta
      181 gatgcaaatt aaggtcatat atagccttat cgataacatg gttaatttta aggataagaa
      241 tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg tatcagcgcc
      301 aacaccaagt tcgatgaatg aaagcacggc gaagggaatc tttaaatatt taaaagaact
      361 gggagtaccc gcgagtgccg ctgatattac ggcgcgagct gaccaggaag gctggaaccc
      421 tgggttcacg gaaaaaatgg ttggatgggc aaaaaaaatg gagacaggtg aacgctctgt
      481 gattaaaaat cctgagtact tttcaacata tatgcaggaa gaactaaaag cactggtctg
      541 agttaaattt atatcagcat aaatgggtca ggacgctttt aatcacatat aaaaagcgtc
      601 ctttttctca caatcaaata atcatcggct tctataacga aatcgatacg gattagtgtg
      661 ttactcgtct tcatcctcaa aacgcgccac gattcgctcg ccggagtggt tggcacgcag
      721 ctcttctgcc accagcgcga ttgcctggcc gctgctcatc ccttgtgcca tcagttcctg
      781 gatccgctcg acagcttttt gctgctgttc atgggtgagt gaaggtaaac ctgcaaacat
      841 tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca catagccagt
      901 agagtcagga ctgatgaaga cgttatctcc cgttgtgatt actttaccct ggcgtcagga
      961 cgccgctgaa ttttatttct cccgcttaag ccacctgccg tgggcgatgc ttttacactc
     1021 cggctatgcc gatcatccgt atagccgctt tgatattgtg gtcgccgatc cgatttgcac
     1081 tttaaccact ttaggtaaag aaaccgttgt tagtgaaagc gaaaaacgca caacgaccac
     1141 tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc gcccaacgca
     1201 taacgaagat ttgccatttc agggcggcgc actggggttg tttggctacg atctgggccg
     1261 ccgttttgag tcactgccag aaattgcgga acaagatatc gttctgccgg atatggcagt
     1321 gggtatctac gattgggcgc tcattgtcga ccaccagcgt catacagttt ctttgctgag
     1381 tcataatgat gtcaatgccc gtcgggcctg gctggaaagc cagcaattct cgccgcagga
     1441 agatttcacg ctcacttccg actggcaatc caatatgacc cgcgagcagt acggcgaaaa
     1501 atttcgccag gtacaggaat atctgcacag cggtgattgc tatcaggtga atctcgccca
     1561 gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc ttaatcaggc
     1621 caaccgcgcg ccatttagcg cttttttacg tcttgaacag ggtgcaattt taagcctttc
     1681 gccagagcgg tttattcttt gtgataatag tgaaatccag acccgcccta ttaaaggcac
     1741 gctaccacgc ctgcccgatc ctcaggaaga tagcaaacaa gcagaaaaac tggcgaactc
     1801 agcgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg atatcggtcg
     1861 tgttgccgta gcaggttcgg taaaagtacc agagctgttc gtggtggaac ccttccctgc
     1921 cgtgcatcat ctggtcagca ccataacggc gcgactacca gaacagttac acgccagcga
     1981 tctgctgcgc gcggcttttc ctggtggttc aataaccggg gctccgaaag tacgggctat
     2041 ggaaattatc gacgaactgg aaccgcagcg acgcaatgcc tggtgcggca gcattggcta
     2101 tttgagcttt tgcggcaaca tggacaccag cattactatc cgcacgctga ctgccattaa
     2161 cggacaaatt ttctgctctg cgggcggtgg aattgtcgcc gatagccagg aagaagcgga
     2221 atatcaggaa acttttgata aagttaataa gatattacgc caactggaga agtaagacgt
     2281 ggaataccgt agcctgacgc ttgatgattt tttatcgcgc tttcaacttt tgcgcccgca
     2341 aattaaccgg gaaaccctaa atcatcgtca ggctgccgtg ttgatcccca tcgtccgtcg
     2401 accgcaaccg gggttgttgc tgactcagcg ttcaattcat ctgcgtaaac acgctggaca
     2461 agtggcattc cctggaggtg cagtcgatga cacggacgca tcagttatcg ccgccgcgct
     2521 gcgcgaagct gaagaagagg ttgctatacc gccttccgcc gttgaagtta tcggcgtgct
     2581 gccgcccgtc gatagcgtaa ctggctacca ggtaacccca gtggtcggca ttatcccgcc
     2641 cgatctgccg tatcgcgcca gtgaagatga agtttccgct gtgtttgaaa tgccgctcgc
     2701 ccaggcatta catctgggtc gttatcaccc tttagatatc taccgccgtg gcgattcaca
     2761 tcgggtatgg ctgtcctggt acgaacagta ttttgtatgg ggaatgaccg caggcataat
     2821 tcgtgagctg gcgctgcaaa ttggtgtgaa accctgacta tacttatctt tacatctaca
     2881 aaacactact tgagacaatc atcgcaatat tagttaaatc gcggtttttg attagtttaa
     2941 ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta cactatgcgc tgttattagt
     3001 tcgttactgg aagtccagtc accttgtcag gagtattatc gtgattagtc tattcgacat
     3061 gtttaaggtg gggattggtc cctcatcttc ccataccgta gggcctatga aggcaggtaa
     3121 acagttcgtc gatgatctgg tcgaaaaagg cttactggat agcattactc gcgttgccgt
     3181 ggacgtttat ggttcactgt cgctgacggg taaaggccac cacaccgata tcgccattat
     3241 tatgggtctt gcaggtaacg aacctgccac cgtggatatc gacagtattc ccggttttat
     3301 tcgcgacgta gaagagcgcg aacgtctgct gctggcacag ggacagcatg aagtggattt
     3361 cccgcgcgac aacgggatgc gttttcataa cggcaacctg ccgctgcatg aaaacggtat
     3421 gcaaatccac gcctataacg gcgatgaagt cgtctacagc aaaacttatt attccatcgg
     3481 cggcggtttt atcgtcgatg aagaacactt tggtcaggat gctgccaacg aagtgagcgt
     3541 gccgtatccg ttcaaatctg ccaccgaact gctcgcgtac tgtaatgaaa ccggctattc
     3601 gctgtctggt ctcgctatgc agaacgaact ggcgctgcac agcaagaaag agatcgacga
     3661 gtatttcgcg catgtctggc aaaccatgca ggcatgtatc gatcgcggga tgaacaccga
     3721 aggtgtactg ccaggcccgc tgcgcgtacc acgtcgtgcg tctgccctgc gccggatgct
     3781 ggtttccagc gataaactgt ctaacgatcc gatgaatgtc attgactggg taaacatgtt
     3841 tgcgctggca gttaacgaag aaaacgccgc cggtggtcgt gtggtaactg cgccaaccaa
     3901 cggtgcctgc ggtatcgttc cggcagtgct ggcttactat gaccacttta ttgaatcggt
     3961 cagcccggac atctataccc gttactttat ggcagcgggc gcgattggtg cattgtataa
     4021 aatgaacgcc tctatttccg gtgcggaagt tggttgccag ggcgaagtgg gtgttgcctg
     4081 ttcaatggct gctgcgggtc ttgcagaact gctgggcggt agcccggaac aggtttgcgt
     4141 ggcggcggaa attggcatgg aacacaacct tggtttaacc tgcgacccgg ttgcagggca
     4201 ggttcaggtg ccgtgcattg agcgtaatgc cattgcctct gtgaaggcga ttaacgccgc
     4261 acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg ctagataagg tcatcgaaac
     4321 gatgtacgaa accggtaagg acatgaacgc caaataccgc gaaacctcac gcggtggtct
     4381 ggcaatcaaa gtccagtgtg actaatactt cttactcgcc catctgcaac ggatgggcga
     4441 atttatcccc gctttctcgt ctgctgtaat attccccact acacttccac tgttgcgtca
     4501 ggcgtttgtc gccatacgct tacagggtgg cccgcatgca aaaagcacaa cggatcatta
     4561 aaacctatcg ccgtaatcga atgattgttt gtacgatttg cgcactcgtt acgctcgctt
     4621 cgaccctgag cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg gtagtacaat
     4681 tcgccaatca cgctgtagaa gaattagata aagtactgct tcccctacag gcaggtagcg
     4741 aagtcttgct tccgctgatt ggtctgccct gctctgtcgc ccatttgcca ttacgtaaac
     4801 aggcggcaaa actccaaact gtgcgatcca ttggcctggt gcaagacggc acactttatt
     4861 gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga cattctggct gaacttcctg
     4921 caccgcaacc acttttacgc ctgacgaccg accgtgtcct gattaaaggc agtccggttt
     4981 tgattcaatg gacgcctgca gcgagcagta gcaatgctgg ggtcatggag atgattaaca
     5041 tcgacttact gacggcaatg ctgcttgagc cacaactgcc gcaaatcagt agcgccagcc
     5101 tgacggtgga caaacggcat ttgctctatg gtaatgggct ggtagattcc cttccacaac
     5161 ctgaagacaa tgaaaactat caggtttctt cgcaacgctt tccttttacc attaacgtta
     5221 atggtccggg ggctacggcg ctggcatggc actatcttcc aacacaatta ccgctggcgg
     5281 tgctgctaag tttactggtg ggctacatcg cctggctggc gaccgcttac cggatgagct
     5341 tttcccgcga aatcaatctg ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt
     5401 tgcttaatgc gcgcagccag caatgtattg gtgtagagat tttgctgcgc tggaacaatc
     5461 cgcgtcaggg ctggatttca ccggatgtgt ttattcctat cgcggaagaa catcatttaa
     5521 ttgtgccact gacccgctat gtgatggcag aaaccattcg tcagcgccat gttttcccga
     5581 tgagtagtca gtttcatgtt ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc
     5641 tgataaaaga tctcaatcag tactggttta gcgctcaccc gattcagcaa ctgatcctcg
     5701 aaatcaccga acgcgatgcc ttactggatg ttgattatcg gattgcccgc gagctgcatc
     5761 gtaaaaacgt caaactggcg attgatgact tcggcaccgg caacagctcg ttttcctggc
     5821 ttgaaacatt acgtcctgac gtgctgaaaa ttgataagtc atttaccgca gctataggtt
     5881 ctgacgcggt taactcgacg gtgaccgata tcatcatcgc gctggggcaa agactgaata
     5941 ttgaactggt ggcggagggc gtggaaacgc aagaacaggc gaagtatttg cgccgtcatg
     6001 gcgtgcatat tttgcaaggg tatttgtacg cacagccgat gccgctacgt gattttccca
     6061 aatggctggc gggcagccaa ccgccgcccg cccggcataa tggacatatc acgcccatta
     6121 tgccgttacg ttaacgctta ctcatcttca tcgtgcgccg gttgctcttt aacaatgcga
     6181 accagatcaa cacgataatc attggcttca atgatggtga tatgcagtgg ccctacatca
     6241 atcacatcgc ccacacgggg aatgtgacca tttgccgaga tcacgaggcc cgcgaccgtc
     6301 gcgatatcat cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa ggcatgcaaa
     6361 tctgtaccgc cttttaccag ccagccgtca ccatcagtaa tgatttccgg cgtttcgtca
     6421 gcgtccggga attcacccgc aatggcttcc agcacatcca gcggcgtgac cagaccttgt
     6481 accacaccaa actcgttggt cacgataaca aagctcccac gagcacgacg cagcacgccc
     6541 aacaggttga tcggatcgag ggtttcaggg acgataatcg ccggagacgc cgaagcaatc
     6601 gccgccacat caacgccctc ttccagcgcc accagcagtt ctttagcacg tacgattccg
     6661 atgatttcat ccagttcacc gcgacatacc gggaacaggc tgtgcggtga ggagagcagc
     6721 tgctcgcgga tttcatctac cccgagatta gcgtcaaccc agcttatttc accgcgcggc
     6781 gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat catgtaacgt
     6841 tcttcttcgg caaatgcacc ttccgggatc ggcatcggca tcgggttatc ggcatcgtgc
     6901 tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc agtacgcgct
     6961 cgcagcggca aagtcgactg gtgacgaata aagttgcgac gcgcaatctg gttaaacact
     7021 tcgatgatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat gtggaaaccg
     7081 aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca gagcaccacc
     7141 accgtggggt gctggttaac aaatcgcgtc agcggtttgg atgccagcaa cataaccgcc
     7201 atcgcaatca ctaccgccgc catcatcacc ggcagatggt taaccatccc tactgcagta
     7261 attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac gactacccag
     7321 aaactggcgt agcctttacc gtggccggaa tcatgatcac ggttttccaa ccgttcatgc
     7381 agttcggttg ttgctttgaa cagcaagaat atccccccga acaacataat caggtcgcgt
     7441 ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac catccatgaa
     7501 atcagcgaca gcagccccag acgcataatc agcgccagtg ataaccccaa caaacgcgct
     7561 ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac caggttatcg
     7621 atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat tgaggggtcc
     7681 attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc agaaataatg
     7741 caggcgtaac gacaaaatgc aaacgaaagg tgcggcatag agtgccagaa aggcaggcgt
     7801 taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct actgccctat
     7861 actccatggt tgttaaacgg gagttaaaca tatcagagac gcctctgatt tggcaaagat
     7921 ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat ttctgctaat
     7981 cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc taaaataaat
     8041 cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat tgtggacgac
     8101 gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg tagcaagtga
     8161 ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt aaaacggcag
     8221 aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca ggtgaaaatg
     8281 ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc actaaaggcg
     8341 tgctgtttct cgttgataca tggggaggca gcccgttcaa tgctgccagc cgcattgtcg
     8401 tcgacaaaga gcattatgaa gtcattgcag gcgttaacat tccaatgctc gtggaaacgt
     8461 taatggcccg tgatgatgac ccaagctttg atgaactggt tgcgctggca gtagaaacag
     8521 gccgtgaagg cgtgaaagca ctgaaagcca aaccggttga aaaagccgcg ccagcacccg
     8581 cggccgcagc accaaaagcg gctccaactc cggcaaaacc aatggggcca aacgactaca
     8641 tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc gccacccgct
     8701 ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg gctgcggata
     8761 ccgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca cacgtagttg
     8821 atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa cgtgtaatgc
     8881 tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg aaaatcacct
     8941 ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaggtgaat aacgcggttt
     9001 cggttgatga aaaagatatc gaggcgttca agaaactgaa tgcgcgcggt attgagctgg
     9061 aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc agcaaaatcg
     9121 ataagtaacg tattgtgttg attatcactc agttttcaca ctttagtctt acgtaaacag
     9181 gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg tagcctgtat
     9241 cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa tcgcgtgtac
     9301 cctggtgggt atcgttcttg gggatatgaa aaccggtatt attatcggtg gtacgctgga
     9361 aatgatcgcg ctgggctgga tgaacatcgg tgctgcagtt gcgcctgacg ccgctctggc
     9421 atctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg caggtattgc
     9481 actggcaatc cctctggccg ctgcgggcca ggtactgacc atcatcgttc gtactattac
     9541 cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga cagcgatttc
     9601 ctggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc cggccgtcat
     9661 cgttgcgctg tctgtaggta ccagcgaagt acagaacatg ctgaatgcga ttccggaagt
     9721 ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt atgcgatggt
     9781 tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct tcgtaaccgc
     9841 agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta tggcagtgct
     9901 ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc aggcagctgg
     9961 taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg atacaactca
    10021 aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc tgcgttctaa
    10081 cctcttccag ggttcatgga acttcgaacg tatgcaggca ctgggtttct gcttctctat
    10141 ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac aagctattcg
    10201 ccgtcacctg gagttcttta acacccagcc gttcgtggct gcgccgattc tcggcgtaac
    10261 cctggcgctg gaagaacagc gtgctaatgg cgcagagatc gacgacggtg ctatcaacgg
    10321 tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct tctggggaac
    10381 cgtacgtccg gtatttgcag cactgggtgc cggtatcgcg atgagcggca gcctgttagg
    10441 tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt actacggcgt
    10501 agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg gcttcctgca
    10561 aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat tggttaacaa
    10621 gtggacacat gtcaacatcc cgctggttgt ctctcgcatt actgaccaga cgggcaaaga
    10681 acacgttact actgtccaga ctattctgga ccagttaatg ccaggcctgg taccactgct
    10741 gctgaccttt gcttgtatgt ggctactgcg caaaaaagtt aacccgctgt ggatcatcgt
    10801 tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac tgtaagactg
    10861 ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa tgacaatcac
    10921 ggacctggta ctgattcttt tcatcgccgc actcctggcc ttcgcgatct acgatcagtt
    10981 catcatgccc cgccgtaacg gccccaccct gctggcaatt cctttgctcc ggcgtggtcg
    11041 catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca acgtcacgaa
    11101 tcatggtgcg ttaataacga catggttatt aagcgcactg gctctgatgg gtttttatat
    11161 attctggatc cgcgttccga agatcatctt taaacaaaaa ggttttttct tcgccaatgt
    11221 ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg tgttggtgat
    11281 gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatc tggaaagaat
    11341 ttataaactt ctcgtttcaa ctcaatgagt tattaattta gccaaagcta tgtttagtgt
    11401 atttttaata atcagacata gcttaggcta tattacctct tc
//
LOCUS       DAZUCE010000162        11360 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZUCE010000162 DAZUCE010000000
VERSION     DAZUCE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.162"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            89..322
                     /locus_tag="ACHY03_003622"
     CDS             89..322
                     /locus_tag="ACHY03_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFS3906808.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            422..1240
                     /locus_tag="ACHY03_003623"
     CDS             422..1240
                     /locus_tag="ACHY03_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS3906809.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            1295..1780
                     /locus_tag="ACHY03_003624"
     CDS             1295..1780
                     /locus_tag="ACHY03_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS3906810.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYSLNNGGAFIAPEPDDNDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1796..2272
                     /locus_tag="ACHY03_003625"
     CDS             1796..2272
                     /locus_tag="ACHY03_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFS3906811.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALAMVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            2341..2562
                     /locus_tag="ACHY03_003626"
     CDS             2341..2562
                     /locus_tag="ACHY03_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS3906812.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2725..3093
                     /locus_tag="ACHY03_003627"
     CDS             2725..3093
                     /locus_tag="ACHY03_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFS3906813.1"
                     /translation="MSDALSGTMLPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            3183..3557
                     /locus_tag="ACHY03_003628"
     CDS             3183..3557
                     /locus_tag="ACHY03_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFS3906814.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            3554..4042
                     /locus_tag="ACHY03_003629"
     CDS             3554..4042
                     /locus_tag="ACHY03_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS3906815.1"
                     /translation="MKLSLMLEADRINVQALNMGQIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            4054..4251
                     /locus_tag="ACHY03_003630"
     CDS             4054..4251
                     /locus_tag="ACHY03_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFS3906816.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            4348..4917
                     /locus_tag="ACHY03_003631"
     CDS             4348..4917
                     /locus_tag="ACHY03_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS3906817.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRSRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFERGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     SAHITFKRLELIDRINDIIARHFPSVLSA"
     gene            5212..6479
                     /locus_tag="ACHY03_003632"
                     /pseudo
     CDS             5212..6479
                     /locus_tag="ACHY03_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(6661..7542)
                     /locus_tag="ACHY03_003633"
     CDS             complement(6661..7542)
                     /locus_tag="ACHY03_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFS3906818.1"
                     /translation="MGMLEREMKNLARQAGGSHKTVHDRIAMAGRFCERLMELNVQIR
                     YVHHLKAKHIEAYIQMRLAQGIQKNTLHNETAAIRKILTQAGREKLAQSERISNKSLG
                     LAGVSRNGTRKAITPEYYQQMAEAARLKDAGLAAALELTRLMGLRSQEAVRCSGSLKT
                     WREALEKGDTRLTVVLGTKGGRPRDTIIQDTGAVKQALDKAIVVAERRNGRLIDAASL
                     KQAMKYWRNQTLRMGLTGKYSPHSLRYAWAQDDIRRYLAQGFSEKEALAMVAMDLGHG
                     DGRGRWVKQVYARNDEA"
     gene            complement(9061..11256)
                     /locus_tag="ACHY03_003634"
     CDS             complement(9061..11256)
                     /locus_tag="ACHY03_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3906819.1"
                     /translation="MSILSIHGVRSYQGERPVEFDLSKYVTLIYGQNGSGKSTVSGYF
                     YKHGRPDYSQCSLRPPLDMNYLVFNQEYVDDIFSQPSQPGIFTLNSENAEIKTEIDAL
                     EAESKVLFARRDALDVQKRDVEGMEESIKNGSAKQIYSSTAEIRKTDLWDLMSGTKQT
                     DKLFQTILEHTEVEDTSTQELTEELHRLEASKGNPYALLEALPASPLNDNDIALLMQP
                     LIPAGDSRLAAAINQLGNIDWVRNGQQWLSDDICPFCQTPIDARQLQQEITALFDTSW
                     EAAMDQLRELQARYQFWHDKPEHMRQLIKTCPLVDQEHPVYLYLLELEQAYQRNKLHI
                     DEKLTSPSASIAVEDLSALAGNVSVQIASINTIISEHNRKAENYQTERVRLKQRLLSH
                     IRKLATDTIINHDEQLAELAEKLAKLTVSRDEITAQLDTLNAIIRGKSSLIVNTQETI
                     ERINHSLDSLGITGFRIAPYDDRDDYRLVREGENSDTPVFSSLSEGEKTLIAFLYFLE
                     TCTGRKSRDDNDQRKRLIVIDDPISSLSQNYVFEIASLIQHQVIRARIGEKVIILTHS
                     LFFFQELLLSAERKKRAAGSCPPEWTLYRVSKSLHSSASLVSEKELLNDYQALWYVLR
                     HAQKDDIASVVIPNTMRQILEYYFGFSGKSETLHRALETLASGPEGEPGFRIFARYLN
                     RHSHQDARNISLHEGASVERYLTWFKKVFEAAKDEEHYTSMMEKTTQTT"
BASE COUNT         2748 a         3020 c         2807 g         2785 t
ORIGIN      
        1 cgttaactga tccctgaagt cagtttcatg cttcagcctc tgtcacaacg cgtggcagag
       61 gctttttatt tatcaggaga aaacagatat gccgggttac acagagtatg tcctggctga
      121 aggatctttc tcatacgggc aggcggttgc tgtcataacc gcttaccgca atgtgtttat
      181 ccaggatgac ccgggaatgc atttccgtcg ggttatccgc aacgcggaag gtcagcgacg
      241 ctggcgctgc cggaattctg aacccgatgc cggaaaggtg cttaatacgc ggctcgccag
      301 tgacggtctt ctcagacaat aaacgtcatt catttcaccc atcaggccgc gtcttctccg
      361 ggagacgtgg ctttttcatt tattacacca gtaaatctta accaccgata aggagcaaat
      421 tatgcgacta gccagtcgtt ttggttatgc gaaccagata cgccgtgacc gtccgctgac
      481 acacgaagaa ctgatgcact atgtgccggg gattttcggg gaagataaac acacgtcccg
      541 aagccagaac tatacgtaca tccccaccat caccgtactg gaaagcctgc agcgggaagg
      601 ctttcagcca ttcttcgcct gccagacccg tgtgcgcgac ccgggccgcc ggggatacac
      661 aaaacacatg ctgcgtctgc ggcgggccgg agagataaac ggagaacatg tccctgaaat
      721 tattctgctc aactctcatg acggtacctc cagctaccag atgctgccgg gttacttcag
      781 gttcgtctgc cagaacggat gcgtctgtgg tcagtctctg ggggaagtgc gtgttccgca
      841 ccggggaaat gtggtggaca gagtcattga aggggcttac gaggtggtgg gggtttttga
      901 ccggattgag gaaaagcgtg atgccatgca gtcactgatt ctgccgccac cggcacgcca
      961 ggcgctggca caggcggcac tgacttaccg ttatggtgac gaacatcagc ccgtcaccac
     1021 cgccgacatt ctgacgccac gacgccggga ggattacggt aaggacctgt ggagtgctta
     1081 tcagaccatt caggagaata tgctgaaagg cggaatttca ggtcgcagcg ctaaaggaaa
     1141 acgtatccat acccgtgcca ttcacagcat tgacactgac attaagctca accgcgcatt
     1201 gtgggtgatg gcggaaacgc tgctggagag cctgcgctga ttcagccatt accctgaaaa
     1261 ccacgcattt atttgtcctt taaggagtca tttcatgaaa accatttcac ataattccac
     1321 cacatcttct gtttccgtaa ccgctgcatc agggaatgac cagcctcagt tggttgccac
     1381 catagtccct gatgaacagc gcatcagctt ctggccgcag cattttggcc tcattccaca
     1441 gtgggtgacc ctggagcccc gtgttttcgg ctggatggac cgtctgtgcg aagactactg
     1501 cggtggtatc tggaatctgt acagtctgaa caacggcggg gcatttatag cgcccgaacc
     1561 ggatgacaat gatgacgaaa catgggtact gttcaatgcc atgaacggta accgcgctga
     1621 aatgagcccg gaagctgccg gtattgccgc ctgtctgatg acgtacagcc atcatgcctg
     1681 tcgtacggag tgttatgcca tgacggtcca ttattaccgg ctgcgggatt acgccctgca
     1741 gcatccggaa tgcagcgcca ttatgcgcat tatcgactga atggaggcag ggacaatgca
     1801 gcagctttcc tttctgcccg gagagatgac gcccggcgag cgcagtctca ttctgcgggc
     1861 cctgaaaacc ctggaccgcc atcttcatga acccggcgtg gccttcacct ccacccgtgc
     1921 ggcacgggaa tggctgattc tgaacatggc gggactggag cgtgaagaat tccgggtgct
     1981 gtatctgaat aaccagaatc agctgattgc cggtgaaacc ctcttcacag gcaccatcaa
     2041 tcgcacggaa gtccatcccc gggaggtgat taaacgcgcc ctgtaccaca atgccgctgc
     2101 cgtggtgctg gcacacaacc acccgtccgg tgaagtcact cccagtaagg cagaccggct
     2161 tattacggaa cgtctggtac aggcactggc catggtggat atccgagtgc cggaccatct
     2221 gatagtcggt ggcagccagg ttttctcctt tgccgaacac ggtctgcttt aacccgtcac
     2281 cgtcacaacc acatcacact tgttttcact tttatcttct gtcttcagag gtatcccacc
     2341 atgaaaatta tcacccgtgg tgaagccatg cgcattcacc gtcagcatcc ggcatcccgt
     2401 ctttttccgt tctgcaccgg taagtaccgc tggcacggca gcactgaggc atataccggt
     2461 cgtgaagtgc aggatattcc cggtgtgctg gccgtgtttg ctgaacgccg taaggacagt
     2521 tttggcccgt atgtccggct gatgagcgtc accctgaact gatgttccgt cacccggaaa
     2581 acgagtacga ctggctgagc tcagacgccg ctgtggatga ggccctgctc ataaacgaat
     2641 acacctttac cgaaggcggt ctgcgcgccg gctgacctgt catcgttaaa ccattcttaa
     2701 ccgattttct gtgaggattt tatcgtgtca gacgcactct ccgggacaat gcttcccgac
     2761 gacaatcacg accacccctg gtgggggctg ccctgcaccg tgacgccctg tttcggggca
     2821 cgtctggtgc aggagggtaa ccgcctgcat taccttgccg accgcgccgg tatcagaggc
     2881 ctgttcagcg atgcagatgc gtaccacctg gaccaggcct ttccgctgct gatgaaacaa
     2941 ctggaactca tgctcaccag cggtgaactg aatccccgcc atcagcatac cgtcacgctg
     3001 tatgcaaaag gtctgacctg caaagctgac actctcggta gcggtggtta cgtttatctg
     3061 gctgtttatc cgacgcccga aacgaaaaag taaaccaccg gaataacctt ctgctccggc
     3121 ctggtgcttt caccacgcca cttttccatt tttcatctct gcatatcagg aaaaccttca
     3181 gtatgaaaac attacccgat acacacgtac gggaggcatc gagctgcccg tctcccgtca
     3241 ccatctggca gactctgctc tcccgactac tgggccagca ttacggcctt acactgaatg
     3301 acacaccgtt cgccgatgaa cgtgtgattg agcagcatat tgaagctggc atttcactgt
     3361 gtgatgcggt gaattttctc gtggaaaaat acgcgctggt gcgtaccgac cagccgggat
     3421 tcagcacttg tccccgctct cagttaataa acagtattga tatcctccgg gctcgccggg
     3481 cgaccggcct gatgacccgc gacaattaca gaacggtaaa taacattacc ctgggtaagc
     3541 atccggagaa acgatgaaac tttccctgat gctggaagcc gacagaatta atgtgcaggc
     3601 actgaacatg gggcaaattg tcgttgacgt cgatggtgtt aatctcactg aactgattaa
     3661 caaggtcgct gaaaacggtt attcactccg cgtggtggag gaatccgacc aacagtcaac
     3721 ctgcacacta ccaccgtttg caacccttgc cggcatacgc tgcagtaccg cacatatcac
     3781 ggaaaaggat aacgcctggc tgtactcgct gtcgcatcag accagtgacg ttggtgaatc
     3841 agaatggatt catttcacag gtagcggata tctgttacgt accgatgcgt ggtcatatcc
     3901 ggttctgcgg cttaaacgcc tggggctgtc aaaaacgttc cgtcgtctgg ttatcacact
     3961 tacccgacgt tatggcgtca gtctcattca tctggatgcc agcgctgaat gcctgccggg
     4021 tttacccact ttcaactggt aaccaggaac aacatgaaat cattaaccac ggaaaccgca
     4081 ctggatattc tgattgcgtg gctgcaggac aatatcgact gcgaatcggg aattatcttt
     4141 gacaacaatg aggataaaac ggattcagca gcactgttgc cctgtatcga acaggccaga
     4201 gaggatatcc gtaccctgcg ccaactgcag cttcagcacc agaaccggtg agtctcactc
     4261 atcatctcac tcaccagact tcattccact gacgccagcc tgaacacggc tggcgttttc
     4321 atttatctgc aaaaaggaat atcgattatg tctgaaatca cagtctcccg tccggaagtg
     4381 gtcaacgaga atacggacgt tatctgctcc acctcagtca ggtccaggtc actggaatat
     4441 gataattttc cggaaatcag cgaagcgaac attctgagca catttgaaca actgcaccag
     4501 aacaaagatg aagtgtttga acggggagtg atcaacgtct tcaaagggct gagctgggat
     4561 tacaaaacca actcaccctg taaatttggc agtaaaatta tcgtcaacaa tctggtgaga
     4621 tgggaccagt ggggatttca tcttatcagt ggaatgcagg cagatcgcct ggctgacctg
     4681 gaaagaatgt tgcatctgct cagcggtaaa ccgatccccg acaaccgagg gaatatcacc
     4741 attaatctgg atgaccacat acagtccgtt cagggtaaag gacgctatga agatgagatg
     4801 ttcatcatta aatactttaa gaagggatct gcacacatca ctttcaaaag gctggagctg
     4861 attgacagaa ttaacgatat aatagccagg cactttcctt ctgtgctctc agcctgaccc
     4921 cgagtttgat tccctttcga tatcaaaagg gactgcgggt acaaaagagg gtacatattt
     4981 caccaaacca aacaaaataa actaatatca acatgataga agcattcttc gattccgagt
     5041 ccggcaccac tatttagttt catggcgtct ctatgagtcc tgaaacatca aaaaatcagt
     5101 tagttacaaa gagtctggag taccaacagg tactgccaga ttttttgaca tccatagttt
     5161 ttgggggctt aattgggggc ccgtcggttc gataaactat ggagccccac tatgtccctc
     5221 accgataccg ccatccgtaa tgcaaagccg cttgataaat cctggaaact cagcgatgcg
     5281 cagggtctgt acctgctgat aaagcccaac ggatctaaac tctggcacct caagtaccgc
     5341 ttcggcggta aagagaagaa gctggcgttt ggtgcttacc cggccgtttc tctggcaaat
     5401 gcccgcaagc tgcgcgagga tgcccgctcg gtattgagcg caggcagcga tcccggcgtg
     5461 aaaaaacagc aggagaagtt tgcacgtaaa agcggcaaca cctttgaaga tatcgcccgt
     5521 aagtgggtag cgggcaatat ccgctggaat gaagcccatg ccgccaaagt cctgcgctcg
     5581 ctggagctgc atgtttttcc actgatcggc aaattccggt ctctgatttg aaaactgccg
     5641 atctgctgat cccgctgcgg gtggcggaac gaaaaggtac gctggagacc gctgcacgga
     5701 ttcagcagcg caccacggca atcatgcgtt acgcggtgca ggaggggata atcgacagca
     5761 atccggcgaa cgatctgacc ggggctatcg caccgccgcc caaaaaccac taccccgccc
     5821 tgccgctgga gaaaataccg gagatgctgg agcgtattga aggctattcc ggcaggctgt
     5881 taacccgtct ggcggtacag cttaatctgc tgatctttat ccgttccagc gagctacgtt
     5941 ttgcccgctg gacggagatc aatctggatg gcgcaatgtg gaccatcccg gcggagcggg
     6001 agccgattcc gggcgtgcgt tattcaaatc gcggcgcaaa gatgaagatg cagcatctgg
     6061 tgccgttgtc agcgcaggcg gtgacaatac tcaaacagtt aaaactgttg tccggcagta
     6121 gtgagttact gtttcccggc gaccacgatc cgcgtaagcc gatgagcgaa aacaccatca
     6181 acaaagcact gcgggttatg ggctacaaca cacaggcaga tgtttgcggt cacggtttcc
     6241 gcacgatggc ctgtagtgcg ctgatcgagt ccgggcgctg gtcaaaagac gccgtggagc
     6301 gacaaatgag ccatcaggag cgtaatcacg ttcgtgccgc gtatatccac aaggcggagc
     6361 acctcgacga gcgcacacag atgatgcagt ggtggtcgga ttatctcgac gcctgtcggc
     6421 agaaatttat cccgccgtat cggtatgaca ggcagattca ggcggcctgc gccagctaac
     6481 ggacaatgcc ggaaagcaga tccactgcat cagggatctg ctttttattt gcgccagata
     6541 tccgtggata gacgtcgatc tgcgccagtc ccaacccttt ggccctttcc ggtaaggagc
     6601 tgttaccctt aacccacctt taccctttgg cctttttcct ttcgctgcgc cagcaaaaaa
     6661 tcacgcttca tcattccgtg catacacctg cttcacccag cgaccacgcc cgtcgccatg
     6721 ccctaggtcc attgccacca tcgccagcgc ttctttttcg ctgaacccct gcgccagata
     6781 ccggcggata tcgtcctgcg cccaggcgta gcgcagtgag tgcggggagt atttgcccgt
     6841 cagccccata cgcaaagtct ggttgcgcca gtatttcatt gcctgtttaa ggctcgccgc
     6901 atcaatcagc ctgccgttgc gccgttctgc caccacgatg gccttatcca gcgcctgttt
     6961 aactgcgcca gtatcctgaa taatcgtgtc acgggggcgt ccacctttgg taccgagtac
     7021 cacggttagc cgggtatcac ctttctccag tgcttcgcgc caggttttca gcgaaccgga
     7081 acagcgtact gcctcctgag agcgtagccc catcagccgc gtcagttcca gcgccgccgc
     7141 cagccccgca tctttcagcc gggcagcctc tgccatctgt tgataatatt ccggcgtgat
     7201 ggctttccgc gtaccattgc gggatacgcc agccagcccc agcgatttat tcgatatccg
     7261 ttcactctgc gccagctttt cgcgcccggc ctgcgtcagg attttacgga tagccgccgt
     7321 ttcgttatgc agcgtatttt tctgaatccc ctgcgccagc cgcatctgaa tatacgcctc
     7381 aatatgcttc gcctttaaat ggtggacata gcgtatctga acgttcagtt ccatcaggcg
     7441 ttcgcagaaa cgtcctgcca tggcgatacg gtcatggaca gtcttgtgcg acccgccagc
     7501 ctgccgcgcc aggtttttca tctctctttc cagcataccc attttacgtt ttcctcttct
     7561 ctcgctcctg attttcacaa aaccacaaca aacaacggat acagctttcc ccggcgcgta
     7621 ggccgatagc ccctgatttt tgtcggggct gcgagcagta cctgagcgcc ggggcgacgg
     7681 ctgttgaggt tttgcggggt atcggttgtg ctctcagagc agccgccagc gtgagctggt
     7741 catcccccgg accgtcctgg tggtccccct ccggtactgg ctcatgttct ccttttgtga
     7801 atgaatggtt gagataaatg ctgaaaagcg acgtcagaag ctcgcctgtg gctggaacag
     7861 ttgatatgac tggtgcagat ggaaatttct gcgggcgtca gtctctcact gcgcgtgagc
     7921 gactgacgcc ctggtctggc gcagggtctg aaaattaaca ctctggtgca ggatgacggt
     7981 cacgcggcag caaagccttc ggcaatgcag gccgcagccc gtcatgtaaa aaggcgctgg
     8041 ttatcagcag ggtataagtg ggctaggaaa attccggcaa agagaggccg ttcagccggt
     8101 gaaggctgtt gccgttaagc gatgacgatg gagccgtcca tgtgtatgta ctcttagcct
     8161 caaacgcttt cgcgttccgg tattgcggag gtcgtaccgg tagaaagggc tggtcatcct
     8221 gataaatcag gggcttcttg cagaccatga agcaagggcg ttgtccacga caacatagtg
     8281 caggagggag attacgcctt tatttctggc gcagaaaggg gaaactcatt tttctggaaa
     8341 tagagttgtt tgtagatttt ctcgcgattt tgtgaatgta aatctacaat gcttcacaca
     8401 cctttccagc caagcaatat cgtacggtcc attcgtcgat aagcctctca ccatcaatgg
     8461 tacatacccg gtaccgcatc ttcattttcc acaacgaagt agtagaccat gtcatagaac
     8521 aattcaccaa gcggcttcta cattggctac atcaaatgta agccatggca acgtcagaca
     8581 agcgaatgac tgccacaaag caccatatcc aatcagcgtt taagcaacgt tttgtgataa
     8641 tcggccagca tcgtaaaata agcaagagag tttccaggaa accatcacgg ttcccgaatt
     8701 ggtcatttca gcagcactat agctaagaca tacagtgcta atactaaaat acgaccatct
     8761 caattaattg ttatccaaag caaagattga atcatcaaat gaaatcgcct acgatagcgg
     8821 aagccatccc ccgggatcct tagcctctac acgatagtca cgctcgtacg aggtgtacgg
     8881 ggctcgccga cagcttgtcg gcgtcgaaag aaaggagccc ggtatgaaac agaaaactgt
     8941 gctggttcac cagtatctgc gtttccgttt tggtcgctgg gagcacgtgt gtcagcacat
     9001 gcgctctttg ccgaactgct aacggttaat cccggggtga tggtcctctt ctgttgttaa
     9061 tcaggtcgtt tgtgtcgtct tttccatcat tgatgtgtaa tgctcttcat cctttgccgc
     9121 ctcgaatact ttcttaaacc aggtaagata gcgttcaacc gaagccccct catgaagaga
     9181 gatattgcgg gcatcctggt gagaatgtcg atttagataa cgagcaaata tacggaaacc
     9241 aggttcacct tcaggcccgc tcgccagtgt ttcaagcgcc cggtgaagcg tctcactttt
     9301 ccccgaaaaa ccaaaatagt attcaaggat ttgccgcatc gtatttggga tcaccacact
     9361 ggcaatgtca tctttttggg cgtggcgtaa gacataccag agtgcctgat agtcgttcag
     9421 taattctttt tcggaaacaa gggaagcaga actatgtaag gatttggata cccgataaag
     9481 cgtccattct ggtgggcaag aacccgctgc ccttttcttc cgttccgcag aaagcagtaa
     9541 ctcctgaaag aaaaacaggc tgtgcgtcag aataatcact ttctcaccaa ttctggcacg
     9601 tataacctga tgctggatca acgatgctat ttcaaaaaca tagttttgcg acaggctgga
     9661 tataggatca tcaatgacaa ttaaacgttt tcgttggtca ttgtcatcac gactttttct
     9721 ccctgtgcaa gtctccagaa aatagagaaa agcaatcagc gtcttttccc cttcactcag
     9781 actactaaaa acaggcgtat cgctgttctc accttccctc accagacgat aatcatcccg
     9841 atcgtcatag ggagcaatgc ggaatccagt gattccaagg ctatccaagc tgtgattaat
     9901 acgttcaatc gtttcctgcg tattgacgat caggctggat ttaccgcgaa taattgcgtt
     9961 cagcgtatca agctgggccg taatttcatc ccgagacacc gttaattttg ccaatttttc
    10021 agcaagttct gctagttgtt catcatggtt aattatcgta tcggtagcca gttttcggat
    10081 atgactgagt agtctctgtt tcagtctaac ccgttcagtc tgataatttt cagcctttcg
    10141 gttatgttcg cttattattg tattaatact ggctatctgc acagagacat tgccagccag
    10201 agcagaaaga tcctcaacag caatcgacgc agagggtgat gtcaatttct catcgatatg
    10261 caatttattt ctttgatacg cctgctccag ttcaagtaga tagagataaa caggatgttc
    10321 ctgatcaact aacggacacg tttttattaa ctgccgcata tgttcgggtt tatcgtgcca
    10381 gaattgatat cgcgcctgaa gttcccgcaa ctggtccatc gcagcttccc atgaggtatc
    10441 aaaaagggca gtgatttcct gttgaagttg ccgggcatct atcggcgttt gacaaaatgg
    10501 gcaaatgtca tcactcagcc actgctgtcc attcctcacc cagtcaatgt tgcccaattg
    10561 gttaattgcg gcagcaagac ggctatcacc tgctggaata agaggttgca tcaatagcgc
    10621 aatatcattg tcattcaaag gggatgctgg caatgcttcg agcaaagcgt aagggttgcc
    10681 tttactggct tcaagtcggt gtagttcttc ggttaattcc tgcgtcgaag tatcttcgac
    10741 ttcagtgtgt tcaaggatgg tctgaaaaag tttgtcggtc tgtttagttc cagacatcaa
    10801 atcccataaa tctgtttttc tgatttctgc ggtagaacta taaatctgtt ttgcggagcc
    10861 atttttaata ctttcttcca tgccctcaac gtcgcgtttt tgcacgtcca aagcatcacg
    10921 ccgggcaaac aaaactttac tctccgcttc cagagcatct atttcagttt taatttctgc
    10981 attttcacta tttaaagtga aaatgcccgg ctgagaaggt tggctgaaaa tatcgtctac
    11041 atactcctga ttaaacacca ggtagttcat atccagtgga ggtcgcaggc tgcactggct
    11101 gtaatctgga cgaccatgct tataaaagta accagaaacg gttgattttc ctgagccgtt
    11161 ttgtccatag ataagagtga catacttact caagtcgaac tccactggcc tttcgccctg
    11221 ataactgcga acgccgtgga tggagagaat gctcatgcta tatccttatc accgcctgct
    11281 gtatgtgggt attttatcgt cggaatttcc tacagcaagc tacctcactg atatctgcca
    11341 ttttaaacat tgtcatagaa
//
LOCUS       DAZUCE010000163        11359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZUCE010000163 DAZUCE010000000
VERSION     DAZUCE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.163"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..303
                     /locus_tag="ACHY03_003635"
     CDS             <1..303
                     /locus_tag="ACHY03_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFS3906820.1"
                     /translation="FQRENPMSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLESRL
                     DAEVDAILDTIQGEQPGEQPEPEPQPEDITSENGDIQPESPVVDATEAQPEPGTML"
     gene            379..594
                     /locus_tag="ACHY03_003636"
     CDS             379..594
                     /locus_tag="ACHY03_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS3906821.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            598..846
                     /locus_tag="ACHY03_003637"
                     /pseudo
     CDS             598..846
                     /locus_tag="ACHY03_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            1901..2434
                     /gene="rrrQ"
                     /locus_tag="ACHY03_003638"
     CDS             1901..2434
                     /gene="rrrQ"
                     /locus_tag="ACHY03_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS3906822.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKKKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2655..2768
                     /locus_tag="ACHY03_003639"
     CDS             2655..2768
                     /locus_tag="ACHY03_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906823.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            2770..3237
                     /locus_tag="ACHY03_003640"
     CDS             2770..3237
                     /locus_tag="ACHY03_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS3906824.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYCDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGHRSLRINATCPGP
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQEYIRTQCIN"
     gene            3261..3485
                     /locus_tag="ACHY03_003641"
     CDS             3261..3485
                     /locus_tag="ACHY03_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906825.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            3830..4156
                     /locus_tag="ACHY03_003642"
     CDS             3830..4156
                     /locus_tag="ACHY03_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFS3906826.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(4288..4488)
                     /locus_tag="ACHY03_003643"
     CDS             complement(4288..4488)
                     /locus_tag="ACHY03_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS3906827.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            4530..4895
                     /locus_tag="ACHY03_003644"
     CDS             4530..4895
                     /locus_tag="ACHY03_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS3906828.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            5179..5736
                     /locus_tag="ACHY03_003645"
     CDS             5179..5736
                     /locus_tag="ACHY03_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFS3906829.1"
                     /translation="MLTTQKRKFALALMSGKNRTAAAVEAGYSEKSARCKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQACHREVPPAEKKNQVSVPPDDGQSFSPLSG
                     IDYMEDGLPDPVKAMGRILVENISIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGDAA
                     KKVANRFAVPPPPRLVVNNDNEGNG"
     gene            5733..7394
                     /locus_tag="ACHY03_003646"
     CDS             5733..7394
                     /locus_tag="ACHY03_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFS3906830.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGVHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWTGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            7457..9394
                     /locus_tag="ACHY03_003647"
     CDS             7457..9394
                     /locus_tag="ACHY03_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFS3906831.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRQFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALV
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGKASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            9439..9660
                     /locus_tag="ACHY03_003648"
     CDS             9439..9660
                     /locus_tag="ACHY03_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906832.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            9606..>11359
                     /locus_tag="ACHY03_003649"
     CDS             9606..>11359
                     /locus_tag="ACHY03_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS3906833.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWNRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASTGKTALAQPPDGASDGNKAISETEHDAVKAMFRG
                     DTAKMTERELSIIRALGEEFSAVLADLQRTFEGKIAAQAQTFEEKLASLSVVLQKCVT
                     GDDVRPMLEQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDV
                     RPMLEQMVKEAVSHIPVPR"
BASE COUNT         2700 a         2656 c         3421 g         2582 t
ORIGIN      
        1 tttcaacgag aaaatcccat gtcagaaatc acatccctgg tcactgcaga ggcagtgaag
       61 gaagtcctgc gctctgaaga agtcctgagc gcactgaaac agaaactccg ccagaacctt
      121 gagtcgcgtc ttgatgcaga agtggatgcg attctggata cgatacaggg agagcagcca
      181 ggcgaacagc cagagccgga accgcaaccg gaagatatca cctcagaaaa cggcgatatt
      241 caaccggaat caccggtggt tgatgcgact gaagcacaac ccgaaccggg caccatgctg
      301 taagggtgag tcagggccat cagtaaagta ctgctggcct ttttcatgtt gtgagcttcc
      361 gtgtgcggga gacggggtat gtaccagatg gaaaaaatca caacaggtgt gtcatacacc
      421 acgtcagcgg tgggaacggg ctactggttc ctgcagttgc tggacagggt ttccccgtct
      481 cagtgggcgg caataggcgt gctggggagt ctgctgtttg ggctgctgac atatctgacg
      541 aacctgtatt tcaaaattaa agaagaccgg cgtaaggcgg cgcggggaga gtagacgatg
      601 aataaacaat acgaactggt tgttaaaggg ataaatattt acccggataa gattactgtt
      661 actgtggcgc tggaaactgg tgggtatacg tcactgttgt tgccaaatgt ggtgattgat
      721 cttgatcgtg ttgaaggtgc cccgctggag ttttacgaag ctgaggcgaa aaagaaggcg
      781 aagcagtttt tcatggatat tgctgccggg ttatgtgaag ggaacgaacc gttgccggaa
      841 aagcgcctct gttcagaaga gcggtatacc atacaaatta acaatgcata taacacgata
      901 cagtcagaaa aagatgatat cgaatcacgc attgaaaaac tggagaacag cgtcgttgaa
      961 ctgaataaaa aactgtcggt gcagatccct tccgaaggtg aaaaaaaacg ccgtgatgag
     1021 cagttcgcgg cgttttacga ttattgccgg aaagtgatga gcagaaatct cgcagagtgt
     1081 ttccgtattc aaaaaaacag agagcgctct gcaaatagtg agattctgga taatttttta
     1141 aaggggacgc atctcgccag tgattgtttc attgctcatt cttttggtgg ctttcatgag
     1201 gccatcaaac atgatgttga gagaagtcca ggtatcctct gttccattcc cgtaagcatc
     1261 gaaatagaca caaatacgat aaccggtgaa ctcgttaccg ccggagtcgg tatattccgt
     1321 catgagggga acgatgccaa aggttccttt acaaaaatcg ggccgttctt tctgaaggta
     1381 gagcgatagt ttgttaagta cccatccgac agctcgctca ttttggttta ttttcgtatc
     1441 gcgaaacaaa aattcgaagc ggccagatga cgcatgggtg caaccaaata ccggggcgta
     1501 ttcatcaacc tttggtgttc ctgaaaacat gcagtccgtg ctctcgaatg cagatgaatt
     1561 gctgttgatc cacttaacaa aagaaaaaaa ggtctgaatc gcttgtctgt ctgaatattc
     1621 aacaaaatcc tcaaggtgtg tctcgatcag ttccgggtgt ttacgaaagt cataatattg
     1681 accgggacga tatttatgag tggttgtttt atcccatggt tgtgctctgc gtccctgtaa
     1741 acggaggtct gccgctggat agataagcat aagtaatctc ctggtggaat gtaatccacg
     1801 atcatccggg gaaattaaga acccgccagt gcccaccact ggtggggtga aggcttaaca
     1861 tatccaggga ttcggaaccg ataaatcctg ataaatatcc atgaacacca aaatcaaata
     1921 cggcctgtcg gctgccgttc tggcgctgat tgccgcaggg gcgcctgcgc ctgatattct
     1981 cgaccagttt ctggatgaaa aagaaggtaa ccacaccacg gcataccgtg atggtgcggg
     2041 tatctggacc atttgtcgtg gtgccaccag ggtggatggc aaacctgtcg tcccgggcat
     2101 gaagttgtcg aagaaaaaat gcgaccaggt taacgccatt gagcgtgata aggcgctggc
     2161 atgggtggag aaaaacatcc gggtgccact gactgaaccc cagaaagcgg ggatcgcgtc
     2221 attctgcccg tataacattg gccccggtaa gtgtttcccg tcgacgtttt ataaacgaat
     2281 taatgcaggc gatcgcaggg gagcgtgtga agcgattcgc tggtggatta aggacggtgg
     2341 cagggactgc cgtatccgct caaataattg ttatggtcag gtatcccgtc gtgaccagga
     2401 gagcgcgctg gcgtgctggg gtatcgacag ataagcagaa tattttgctg aaaaatgagg
     2461 tttgcttaca tggacggata acacgaaatc ctgcgaactg gcaaaatgta agtgaataaa
     2521 agtaaaaacc ccgtttgttg gcagcaagcg gggttttgtg tttctgacct tggataaggc
     2581 aagggagaaa ttatgggtag ggaggtgctt tccctgtgag gaagtataaa agattctttc
     2641 tgaggttgtc cattatgaaa ggcattgaag tggagacgcc agccagtctg gatttaacaa
     2701 gagcggcagc ttttgccatt cgtattgtgg ccattgctgt tctggtctgg gcaatccgtt
     2761 ggtggtgata tgaaccgtgt tctgtgtgtg gtgattattg tcctgctggt agcctgtggt
     2821 gtgcttagtc tggggctgaa tcattactgc gataacgcca tcacctataa agcgcagcgc
     2881 gataaaaaag ccagagagct ggagctggca aacgcaacca ttactgacat gcagcagcgc
     2941 cagcgtgatg ttgcagcgct cgatgccaga tactcgaggg aattagccga tgcgagagct
     3001 gaaaatgaaa ctctgcgtgc tgacgttgcc gctggtcacc gcagcctgcg gatcaacgcc
     3061 acctgtccag gtcccgtgcg tgaagccacc ggcacctccg gtgtggataa tgcaaccggg
     3121 ccccgactgg cagacaccgc tgaacgggat tatttcatcc tcagagaacg gttgatgaca
     3181 atgcagaagc agctggaagg ggcgcaggaa tatatccgca ctcagtgtat taactagtat
     3241 ttttgttatc cggaggatgc atgaagaaat tactggtaac cgtaaagcct tttcagggaa
     3301 caattccgtt ccgtattttg cagcgtggtc gtgttcttgt tgaaggttcg ttcagtggta
     3361 aatgtacgca attacactcc cggacctttc aggtgaatgc cacgaatgaa gagctaaccg
     3421 ttgagtgtac gatgaatgcc gctaaatgcc gtatggtatc ggctgcatta cagccagtgt
     3481 gttgagcgac cttattatcc atgcgcggta ttgtcgccgt attcccgtat taacagagac
     3541 cgcagcccga cagggagact cctctgcgcg agtgtgcggg gataatcaaa aacgatacac
     3601 accggggttt accgcgttaa cggagcgcgg cgttgtcctc tcatggtcgc tggtccggtg
     3661 cgatggtgga agaaacctga cgatgtgtta cctcacaaga cctgttatgt catgtgtctg
     3721 atttgtgatt taagtcggat aattgtcgtt gccattaagc agaggattga tggccgacag
     3781 ggtggcattg ttagaataag acttattctt atctgtgccg ggaatgaaaa tgaaaagaaa
     3841 tcttccgtta attattttgt tgtcttctct ggttatgggc tgtacgcaac ataaaacaga
     3901 tatgccccga cagttggtta aggcattacc acaatatccg gcctatgcag cggcaaatta
     3961 tataaaggga cgggttgatg tgaagtttga tattggtgct gatggtactg tcacccgaat
     4021 tgagtttatc cgttcagagc cgcaccatct gtttgatgag caggttgtaa aggcgatggc
     4081 aaaatggcga tttgagaagg acaggccgcg taaaggcgtg aagaaaacgt ttatctttag
     4141 tccttctgca ccctgattat ttcatcagaa attaattatc actctgttgt tattctgtac
     4201 atcccggctg ggtaagtctt gttccgccgg gtatgaagat gaaatattgt tggaggacag
     4261 tgggtacctg ctcctgtaac cgaacgttca tttctcgtta tttgtcatgc tggccggacg
     4321 cagatgcgtt gcatctgttg ccagccttct cctgcaggct tcaataaccc acgctgaaaa
     4381 gttaccggac cctttatgct caagggcgat gttgatctgt tcaatcatgt gattggggaa
     4441 acggatattg cgggttgtgg ttctgcgggt ccggtttttc gatgacatat ttatttcctt
     4501 tactgattgc catatgacgg ggattttaca tggctgagct tcgtacactc cagagcagaa
     4561 tcaaaacact gaatacccga cgggtgaata ttctgaaggg tgaacagcgt cgtgtcagtg
     4621 gcagtgcacg tgtttccctc aagcgtcata tctggctcag ggatgccggg cagtgctgtc
     4681 tctgtggtcg tgtggttgac ctctgtgaca gtgaactcga tcaccgaatt gcacttcagt
     4741 tcggtggtgg taatgaggag acgaatctct ggacgctctg taccgaatgc catcgacaaa
     4801 agtctgctcg tgaagcggcg ggtggtatgc cggacccgac gctgccggag gtgtccggag
     4861 gtagtggcag agcggacgac atcatcggac tgtaacccga ccgggggggg tatcattcgg
     4921 cgtaaaaaac gatcgctccg gacaccgcgc cccctctcac gcagagaaaa aattcccctt
     4981 cagaagttat aaacatgttt ataacctact gatggatttt taccgttttt ttctttttat
     5041 tcataacgtt gtgggaggta aatgtttata cccttttttc gcaaaatgtt tacagggcag
     5101 cggtaaacat ttgccctgcc gcctccattc ttaccgggag gattgctgac gtttttctgt
     5161 ttttccccgg aggaaagaat gctgacgacg caaaaaagaa aatttgcgct ggcgctcatg
     5221 tcgggcaaaa acagaacggc tgctgcagtt gaggctggtt actcggaaaa gtccgccaga
     5281 tgtaaaggct cacaactggc aaaagatccg gaagtgctcg cgtttattgc ccgtaagcag
     5341 ggcgagacgg tggaggtgga tgaggttcct gtttaccggc agaaaaagac gcaggcgtgc
     5401 caccgggaag ttcctcctgc ggaaaagaaa aaccaggttt cagtgccgcc tgatgacggg
     5461 caaagtttct ctccactatc cggtattgat tacatggagg acggccttcc tgatcctgtg
     5521 aaagccatgg ggcggatcct ggtggaaaac atcagcattg atccgaaact ggctctggat
     5581 gcagcctggc gactggcgca gttcacgcac cacaaaaaag gggatgccgg taaaaaatcg
     5641 gcaaaaggtg acgcggcgaa aaaagtggct aaccgttttg cggtgccacc accaccccgc
     5701 ctggtggtga ataatgataa tgagggcaac ggatgatacc tgtgtggagc acggcctgcc
     5761 cggactgggc agagcgcctg aaaaaggggc tgtcgattat tccggctccg atttacccgg
     5821 accaggccgc acatgccctg gcgattttta aacaactgcg gattgtggat gcaccgggca
     5881 gcccgacgtt cggggagtcc tgtgcaccgt gggtgtttga tctggtggct gccctgtttg
     5941 gctcctacga tgcgcagacc ggtgtacgcc atataaagga agtgttcatt ctgataccca
     6001 aaaaaaacag caagtccacg ctggctgccg ggatcatgat gacggcgctg ttactgaact
     6061 ggcggcaggc ggcgggctac accattctgg ccccgaccgt ggaggtggcg gctaacgcct
     6121 tcaaccctgc cagggatatg gtacgacggg acgatgatct ggatgacctc tgtcaggtgc
     6181 agacacatat ccggaccatc acccacaggg tgacggacac cacccttaag gtggtggctg
     6241 ccgatccgaa tacggtatcc ggtatcaagt ccgtggggac gctgattgat gaactgtggt
     6301 tatttggcaa gcagtacaaa gcggaggaca tgttacgtga agccataggc ggccttgcct
     6361 cccgcccgga agggtttgtg gtgtatacga ccacccagtc gaatgagccg ccagccgggg
     6421 tgttcagaca gaaactgcag tacgcccggg atgtccgtga cggcaaaatt catgatccgc
     6481 actttctgcc ggtgatattt gaacaccctc ctgaaatggt ggaaagcggg gttcacctgc
     6541 tgatggaaaa cctcgccatg gtcaatccga atctcggtta ttcggtggat gaggcttttc
     6601 tgtaccggga gtaccgtaaa gcccgggagg ctggtgagga agcatttcgt ggcttcatgt
     6661 caaaacatgc caatgtggaa attggtcttg ccctgcgttc tgaccgctgg acgggtgcgg
     6721 atttctggga gcagcagggc aggcgcgtca gcctggacga tatcctgcag cgcgctgatg
     6781 tggtgacggt ggggattgac ggcgggggcc tggatgatct gctgggaatg tacgtgattg
     6841 gccgtgacag ggaaacccgc gaatggctgg gctggggcca tgcctgggcg catgaaaccg
     6901 cggtggtcag acggaagagt gaggcatccc ggtttcagga ttttgtggcc tgtggagata
     6961 tgacgattgt ccggcgggtc ggggatgaca cggcggaagt ggcggagtat gtgcgtcgta
     7021 ttcatgaggc tgagttactg gagcatatcg gtattgaccc gtcaggtgtg gggcagattc
     7081 tggattcact ggcggaagct gggatccccg acggaattgt tgtggggata agccagggct
     7141 ggaagctggg cggtgccata aaaaccaccg agcgcaaact ggctgaggga gtgctggtgc
     7201 atggtggtca gccactgatg gcctggtgcg ttggcaatgc ccgggtggag cctaaaggta
     7261 acgccatcct tattaccaaa caggccagcg gacgggggaa aattgacccg ctgatggcgc
     7321 tcttcaatgc ggtatccctg atgtccctga atccggagcc gaaaaagaaa gaatatgcgg
     7381 tttttttcat ataaccctgt tcaccctgta accatcatgg accctgcggc ggttttttta
     7441 ttttcaggag gctgatgtga ctcttaaacg ggcctgctcc ctgctgacgg tgaaatcctt
     7501 cagtgaggat gagcgggtga tcaccgggat tgcgtcaacg ccttctccgg atcgggatgg
     7561 tgacatcctg gagccggagg gcgcggagtt tggcagtgcg atcccgtttc tctggcagca
     7621 tgaccattcc cgcccggtgg ggcagtgtac ggtgcgccgg gtcagcgaag ggctggaaat
     7681 cacggcaaca ctggcgaagc ccgtgccgga tatgccatcg caactggctg ccaggctgga
     7741 tgaggcctgg gcggccatta agaccgggct ggtcaggggg ctgtccgtgg gcttccgtcc
     7801 tcatgaatac acctttctgg acggaggcgg actgcatttt ctgcgctggg agctgatgga
     7861 ggtgtctgcc gtcaccgtgc ccgcgaatgc ggaatgcacc atccggacca ttaaatctta
     7921 cgaccgccag ttttctgccg cgtccggcaa ccggaaaccg gtggtgaaaa tcgcatcttc
     7981 tgccggcgct gcggcacagt caacaaccgt ttttcataag gaaaagacca taatgaatat
     8041 tggcgaacag attaaaagtt ttgaaaacaa gcgtgcagca ctggcagcct cccttgagga
     8101 ggtcatgacc aaagccgcag aggaagggcg cacgctggat gtggaggagg aagagcatta
     8161 cgacaacacc gcagcggaaa tacgtcaggt ggatgcgcac ctgaagcgcc tgcgtgaact
     8221 ggaagccggt aaggccgcca cggcgcagcc ggtgaaacag gccggtaacg ggaatgtggc
     8281 cgcggtggct tctgcgccgg tgatccgtgt ggagcagaaa ctggataagg ggattggttt
     8341 cgcacgtttt gccaaatcgc tggctgcggc taaaggtgtc cgctctgaag ccctggaagt
     8401 ggcccgtcgt cagtatccgg atgacagtcg tctgcatcat gtcctgaaat cggcagtggg
     8461 cgcggggacc accacggatc cgcagtgggc aggcagcctg tctgaatatc aggaatacgc
     8521 acaggacttt attgattacc tgcgtcctca gaccattatc gggcgatttg gtcagggcgg
     8581 gatccctgca cttcgtcagg tgccgttcaa tatccgtgtg cacgcccagg tgtccggcgg
     8641 tgctgccggc tgggtgggtg agggtaaggc aaaacccctg acgaagtttg attttgaatc
     8701 catcaccttc agtcatgcga aggtgtcggc cattgcggta ctgacggaag aattgatccg
     8761 tttttccagt ccggctgctg atgcactggt ccgtaatgcg ctggtggaag cggtagtggc
     8821 gcgtctggat acagactttg tggacccgaa aaaagccgca gtggcagatg tctccccggc
     8881 gtccatcacc catgatgtga agggcaaggc atcaaccggt aacccggatg cggatgcaga
     8941 ggcggcgttt ggacagtttg tggcagcaaa cctgcagccc accggtgcgg tctggctgat
     9001 gtccagcacc aatgccctgg cgctgtccat gcgtaaaaat gcgctgggtc agaaagaata
     9061 cccggacatg accctgctgg gtggctcctt tcaggggctg ccggtgattg tctcccagta
     9121 cgtgggtgac cagctggtgc tggtgaatgc cccggatatt tatctggcgg atgacggcgg
     9181 cgtggcagtg gatatgtccc gcgaggcatc actggaaatg cagtctgagc cgaccggcga
     9241 cagtaccacg ccgtcgccgg tggagctggt ttccatgttc cagacaggca gcgtggccat
     9301 ccgtgcggag cgctggatca actggcgtcg tcgccgtacc gcggcggtgg cggtgatcac
     9361 aggagtgaac tacggcagtg cgtccggcgg ctgagtctga taaggaggac gggaggcgtg
     9421 cgcctcccgt aacaggttat ggcaaagatc cgatatctgc agggcacgca tgatgcccgg
     9481 gccggggata tccgtgatgt ggcacagccg tgtgcggagg tgctggttcg cctgggaaag
     9541 gcggagtaca tcacggtgcg acgtccggca ggtcagaaaa agaaacgtga tgcggagcat
     9601 ggcgaatgtg gaacctttta cggcgaaccc gaaaaaacca gaaatcagga cgtgacgtaa
     9661 gagaggcggg ctggaccagc ctgtttcagg cggtggctga gcccttttcc ggcgcctggc
     9721 agcagggcgt gaaagccgat cctgaagccg tcctctcctt tcatgcggtg tttgcatgta
     9781 tttcgctgat atcccaggat atcgccaaaa tgcggctgcg tcttatgcag acggatgcgc
     9841 atgggatacg cagggaaacg cgccgggggg atattgcccg cctctgtcgt cgccccaatg
     9901 cgcagcagaa ccgcatccag ttttttgaac tgtggctgaa cgccaaactg cgtcacggca
     9961 atacggtggt gctgaaaatc cgtaatgccc gggggcagat caaagaactg cgtattctgg
    10021 actggaaccg ggttgaacct ctggtggcgg atgacggcga ggtgttctac cgcatcacgc
    10081 cggaccggaa ctgtgggatc actgaggcgg tgacggtgcc tgcccgggaa gtgatccacg
    10141 atcggtttaa ctgttttttt catccgctta tagggttgcc gccggtgtat gccgccgggc
    10201 tggcggccac gcaggggcat catattcagg aaaattcgac gtcttttttc agaaatggcg
    10261 gcaggccgtc cggggtgatt gagatccccg gcagtattac ggaagaaaat gcgaaaaaac
    10321 tgaagagcaa ctgggacagc gggtatacag gcgaaaatgc ggggaaaacg gccattctga
    10381 gcaacggggc aaaatacaac cccacgacgt tttcaccggt ggatgcgcag acggtggaac
    10441 aactgaagat gaccgctgaa attgtctgtt cggtgttccg tgtcccggcc tacaagattg
    10501 gcgtgggaca accgccttcc agtgacaatg tggaggcgct ggagcagcag tattattccc
    10561 agtgcctgca gacgctgatt gagtccattg aactgttact ggatgaggcg ctggaaacgg
    10621 gggaaaacga gagtacggaa tttgatgtca ccacgctgct gagaatggac agtgagcggc
    10681 gcatgaaaac gctgggggat gcggtgaaaa atacgcttct cacgcccaat gaggcccgta
    10741 aacgggagaa cctgccgccc ctggccggcg gtgatgcact gtatcttcag cagcagaact
    10801 acagtctgga ggcgctgtcc cgtcgtgatg cccgtgagga tccgttcgcg tcgaccggga
    10861 aaacggcctt agcgcaaccg cctgacggcg catctgacgg taataaggca atcagtgaaa
    10921 cagagcatga tgcggtgaaa gcgatgttca ggggggatac tgcgaaaatg acggaacggg
    10981 aactgtccat tatccgtgcg ctgggtgaag aattttccgc agtgctggcg gatttacagc
    11041 gcacatttga ggggaaaata gccgcgcagg cacaaacgtt tgaagaaaaa ctggcttccc
    11101 tgtctgtggt attacagaag tgcgtgacgg gcgatgatgt gcgtccgatg cttgagcaga
    11161 tggtgaagga ggcggtaagc catatccctg ttccgcgtga cggtcgtgac tacgatccgg
    11221 aagtactgca gaaggcggtg aatgacgcgg tcgcaaatat tccacagcca gcggacggta
    11281 aaagtctcac cccggatgat gtgcgtccga tgcttgaaca gatggtgaag gaggcggtaa
    11341 gccatatccc tgttccgcg
//
LOCUS       DAZUCE010000164        11293 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZUCE010000164 DAZUCE010000000
VERSION     DAZUCE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.164"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            111..1220
                     /gene="livK"
                     /locus_tag="ACHY03_003650"
     CDS             111..1220
                     /gene="livK"
                     /locus_tag="ACHY03_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFS3906834.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1268..2194
                     /gene="livH"
                     /locus_tag="ACHY03_003651"
     CDS             1268..2194
                     /gene="livH"
                     /locus_tag="ACHY03_003651"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFS3906835.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2191..3468
                     /gene="livM"
                     /locus_tag="ACHY03_003652"
     CDS             2191..3468
                     /gene="livM"
                     /locus_tag="ACHY03_003652"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFS3906836.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVRQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3465..4232
                     /gene="livG"
                     /locus_tag="ACHY03_003653"
     CDS             3465..4232
                     /gene="livG"
                     /locus_tag="ACHY03_003653"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFS3906837.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAAIWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4234..4947
                     /gene="livF"
                     /locus_tag="ACHY03_003654"
     CDS             4234..4947
                     /gene="livF"
                     /locus_tag="ACHY03_003654"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFS3906838.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5066..5812
                     /locus_tag="ACHY03_003655"
     CDS             5066..5812
                     /locus_tag="ACHY03_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS3906839.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            6172..7488
                     /gene="ugpB"
                     /locus_tag="ACHY03_003656"
     CDS             6172..7488
                     /gene="ugpB"
                     /locus_tag="ACHY03_003656"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFS3906840.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            7586..8473
                     /gene="ugpA"
                     /locus_tag="ACHY03_003657"
     CDS             7586..8473
                     /gene="ugpA"
                     /locus_tag="ACHY03_003657"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFS3906841.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            8470..9315
                     /gene="ugpE"
                     /locus_tag="ACHY03_003658"
     CDS             8470..9315
                     /gene="ugpE"
                     /locus_tag="ACHY03_003658"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFS3906842.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            9317..10387
                     /gene="ugpC"
                     /locus_tag="ACHY03_003659"
     CDS             9317..10387
                     /gene="ugpC"
                     /locus_tag="ACHY03_003659"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFS3906843.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINNQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMYTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            10384..11127
                     /gene="ugpQ"
                     /locus_tag="ACHY03_003660"
     CDS             10384..11127
                     /gene="ugpQ"
                     /locus_tag="ACHY03_003660"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFS3906844.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGSLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQRAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(11114..>11293)
                     /locus_tag="ACHY03_003661"
     CDS             complement(11114..>11293)
                     /locus_tag="ACHY03_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906845.1"
                     /translation="LNPARQQMLPNTNGGMLNNNHNPDSSLNQQHMLQQRKNGDMLNP
                     PSTPQPDIPLKTIGP"
BASE COUNT         2450 a         2900 c         3253 g         2690 t
ORIGIN      
        1 taaaccctta atcagacagg caaaaacagt gcagtataaa aaaagaacag tctgatttgt
       61 taacacataa aaacaaagca acacaacatc acgaatgggg atttttgact atgaaacgga
      121 atgcgaaaac tatcatcgca gggatgattg cactggcaat ttcacacacc gctttagccg
      181 acgatattaa agttgccgtt gtcggcgcga tgtccggccc gattgcccag tggggcgata
      241 tggaatttaa cggcgcgcgt caggcgatta aagacattaa tgccaaaggg ggaattaaag
      301 gcgataagct ggttggcgtg gaatatgacg acgcctgcga cccgaaacaa gccgttgcgg
      361 tcgccaacaa aatcgttaat gacggcatta aatacgttat tggtcatctg tgttcttctt
      421 ctacccagcc tgcgtcagat atctatgaag acgaaggtat tctgatgatc tcgccgggag
      481 cgaccaaccc ggagctgacc caacgcggtt atcaacacat tatgcgtact gccgggctgg
      541 actcttccca ggggccaacg gcggcaaaat acattcttga gacggtgaag cctcagcgca
      601 tcgccatcat tcacgacaaa caacagtatg gcgaagggct ggcgcgttcg gtgcaggacg
      661 ggctgaaagc ggctaacgcc aacgtcgtct tcttcgacgg tattaccgcc ggggagaaag
      721 atttctccgc gctgatcgcc cgcctgaaaa aagaaaacat cgacttcgtt tactacggcg
      781 gttactaccc ggaaatgggg cagatgctgc gccaggcccg ttccgttggc ctgaaaaccc
      841 agtttatggg gccggaaggt gtgggtaatg cgtcgttgtc gaacattgcc ggtgatgccg
      901 ccgaaggcat gttggtcact atgccaaaac gctatgacca ggatccggca aaccagggca
      961 tcgttgatgc gctgaaagca gacaagaaag atccgtccgg gccttatgtc tggatcacct
     1021 acgcggcggt gcaatctctg gcaactgccc ttgagcgtac cggcagcgat gagccgctgg
     1081 cgctggtgaa agatttaaaa gctaacggtg caaacaccgt gattgggccg ctgaactggg
     1141 atgaaaaagg cgatcttaag ggatttgatt ttggtgtctt ccagtggcac gccgacggtt
     1201 catccacggc agccaagtga aaatcccacc gcccgtgaaa tgcgggcggg tttagaaagg
     1261 ttaccttatg tctgagcagt ttttgtattt cttgcagcag atgtttaacg gcgtcacgct
     1321 gggcagtact tacgcgctga ttgccatcgg ctacaccatg gtttacggca ttatcggcat
     1381 gatcaacttc gcccacggcg aggtttacat gatcggcagc tacgtctcat ttatgatcat
     1441 cgccgcactg atgatgatgg gcattgatac cggctggctg ctggtagcgg cgggattcgt
     1501 cggcgcaatc gtcattgcca gcgcctacgg ctggagtatc gaacgggtgg cttaccgccc
     1561 ggtgcgtaac tctaagcgcc tgattgcact catctcggca atcggtatgt ccatcttcct
     1621 gcaaaactac gtcagcctga ctgaaggttc gcgcgacgtg gcgctgccca gcttgtttaa
     1681 tggtcagtgg gtagtgggtc atagcgaaaa cttctctgcc tctattacca ccatgcaggc
     1741 ggtgatctgg attgtcacct tcctcgccat gctggcgctg acgattttca ttcgctattc
     1801 ccgcatgggg cgcgcctgtc gtgcctgcgc ggaagatctg aaaatggcga gcctgcttgg
     1861 cattaacact gaccgggtga ttgcgctgac ctttgtgatt ggcgcggcga tggcagcggt
     1921 ggcgggcgta ctgctcggtc agttctacgg cgtcattaac ccctatatcg gctttatggc
     1981 cgggatgaaa gcctttaccg ctgcggtgct tggtgggatt ggcagcattc cgggagcgat
     2041 gattggcggg ctgattctgg ggattgcgga ggcgctctct tctgcctatc tgagtacgga
     2101 atataaagat gtggtctcat tcgccctgct gattctggtg ctgctggtga tgccgaccgg
     2161 tattctgggt cgcccggagg tagagaaagt atgaaaccga tgcatattgc aatggcgttg
     2221 ctctctgccg ccatgttttt tgtgctggcg ggcgtcttta tgggcgtaca actggagctg
     2281 gatggcacca aactggtggt cgacacggct tcagatgtcc gttggcagtg ggtgtttatc
     2341 ggcacggcgg tggtcttttt cttccagctt ttgcgcccgg ctttccagaa agggttgaaa
     2401 agcgtttccg gaccgaagtt tattctgccc gcgattgatg gctccacggt gaggcaaaag
     2461 ctgtttctcg tggcgctgct ggtgcttgcg gtggcgtggc cgtttatggt ttcacgcggg
     2521 acggtggata tcgccacctt gaccatgatc tacattatcc tcggcctcgg gctgaacgtg
     2581 gtggtagggc tttctggcct gctggtgctg ggttacggcg gcttttacgc catcggcgct
     2641 tacacctttg cgctgctcaa tcactattac ggcctgggct tctggacctg tctgccgatt
     2701 gccgggttaa tggcagcggc ggcgggcttc ctgctcggtt ttccggtgct gcgcctgcgc
     2761 ggcgactatc tggcgatcgt taccctcggt ttcggcgaaa ttgtgcgcat attgctgctc
     2821 aataacaccg aaattaccgg cggcccgaat ggtatcagtc agatcccgaa accgactttc
     2881 ttcggcctcg agttcagccg taccgcccgt gaaggcggct gggacacgtt cagtaatttc
     2941 tttggcctga aatacgatcc ctccgatcgc gttatcttcc tctacctggt ggcgttgctg
     3001 ctggtggtat taagcctgtt tgtcattaac cgcctgctgc ggatgccgct gggtcgcgcc
     3061 tgggaagcgt tgcgtgaaga cgaaatcgcc tgccgttcgc tgggcttaag cccgcgtcgt
     3121 atcaagctga ccgcctttac catcagcgcc gcgtttgcgg gttttgccgg aacgctgttt
     3181 gcggcgcgtc agggctttgt cagcccggaa tccttcacct ttgctgaatc agcctttgta
     3241 ctggcgatag tggtgctcgg cggtatgggc tcgcaatttg cggtgattct ggcggcaatt
     3301 ttgctggtgg tgtcgcgcga gttgatgcgt gatttcaacg aatacagcat gttaatgctc
     3361 ggtggtttga tggtgctgat gatgatctgg cgtccgcagg gcttgctgcc catgacgcgc
     3421 ccgcaactca agctgaaaaa cggcgcagcg aaaggagagc aggcatgagt cagccattat
     3481 tatctgttaa cggcctgatg atgcgcttcg gcggcctgct ggcagtgaac aacgtcaatc
     3541 ttgaactgta cccgcaggag atcgtctcgt taatcggtcc taacggtgcc ggaaaaacca
     3601 cggtctttaa ctgcctgacc ggattctaca aacccaccgg cggcactatt ttactgcgcg
     3661 atcagcatct ggagggtttg ccggggcagc aaatcgcccg catgggcgtg gtgcgcacct
     3721 tccagcatgt gcgtctgttc cgtgaaatga cggtaattga aaacctgctg gtggcgcagc
     3781 atcagcaact gaaaaccggg ctgttctctg gcctgttgaa aacgccttct ttccgtcgcg
     3841 cccagagcga agcgctcgac cgcgccgcca tctggcttga gcgcattggt ttgctggaac
     3901 acgccaaccg ccaggcgagc aacctggcct acggtgacca gcgccgtctg gagatcgccc
     3961 gctgcatggt gacgcagccg gagattttaa tgctcgacga acctgcggca ggtcttaacc
     4021 cgaaagaaac gaaagagctg gatgagctga ttgccgaact gcgcaatcat cacaacacca
     4081 ctatcttgtt gattgaacac gatatgaagc tggtgatggg aatttcggac cgaatttacg
     4141 tggtcaatca ggggacgccg ctggcaaacg gtacgccgga gcaaatccgt aataacccgg
     4201 acgtgatccg tgcctattta ggtgaggcat aagatggaaa aagtcatgtt gtcctttgac
     4261 aaagtcagcg cccactacgg caaaatccag gcgctgcatg aggtgagcct gcatatcaat
     4321 cagggcgaga ttgtcaccct cattggcgcg aacggggcgg ggaaaaccac cttgcttggc
     4381 acattatgcg gcgatccacg cgccaccagc gggcgaattg tgtttgatga taaagacatt
     4441 accgactggc agacggcgaa aatcatgcgc gaagcggtag cgattgtccc ggaagggcgt
     4501 cgcgtcttct cgcggatgac ggtggaagag aacctggcga tgggcggctt ctttgccgaa
     4561 cgcgaccagt tccaggagcg cataaagtgg gtgtatgaac tgttcccgcg tctgcatgag
     4621 cgccgtgttc agcgggcagg caccatgtcc ggcggtgaac agcagatgct ggcgattggt
     4681 cgcgcgctga tgagcaaccc gcgtttgttg ttgcttgatg agccatcgct cggtcttgcg
     4741 ccgattatca tccagcaaat tttcgacacc atcgagcagc tgcgcgagca ggggatgact
     4801 atcttcctcg tcgagcagaa cgctaaccag gcgctgaagc tcgctgatcg cggctacgtg
     4861 ctggaaaacg gccatgtagt gctctccgat actggtgatg cgctactggc gaacgaagca
     4921 gtgagaagtg cgtatttagg cgggtaaatt atcaataacc cggcagctaa gttctgccgg
     4981 gttattcgtc cgtatacaga cttgtttcaa ctgaccggga ggattaaaac cagaccactt
     5041 atttgccacg gaaaggttcc ctcccatgca ttatgccgtt gttgtcatgc ttgaactgct
     5101 ttgctgtatt cacgcttacc gtaccggaca agagcggtac tggattttta tcattttctg
     5161 ttttccggtg attggctgcg tggcgtattt cgtcatagtg atgctgcctg aaacgggcgc
     5221 tgaccgtcac gggcatactt tgctgatgcg cctgcaggac aaactaaacc ctgagcgcca
     5281 cctgcgcaaa ttaaccgaag aactggcgat tgccgagacg aatcaaaacc attatgcgtt
     5341 ggctaatgaa ctggcgcgtc tggggcgtta tcacgaagct gttccccatt atcagcaggc
     5401 gttaagcggg atattcgccc atgaagcggc aatgatgcta agcctggcgc aggcacagtt
     5461 tgctattcag gagttcgccg cctgtcagca gacgctggaa gatgtgatgc gttataaccc
     5521 tgattttcag tcggcagacg ggcatttgtt atttgccaga acgttagccg cacaagaaaa
     5581 atacgctgat gcggaaagcg agtttgaagt attgatttct tattaccccg gcccgcaggc
     5641 gcgtatttac tatgcggaaa tgttggcgaa aatgagtcgg ttacgtgaag ccaacgagca
     5701 gtatgttgcc gtggttgata cggctaaacg ctcaagacct cattatagaa aacaccaccg
     5761 ggaatggatt aagaccgcca atgagcggct gaagcagagt gtggtgcaat gaaaatcttc
     5821 tttgccgtta gaccagtgtc gcggcaatct tttgttaaaa ttgaaaaatg tagctctcat
     5881 cgggcagcaa aactggacgc agaatttgct gccatttttg ctcttcatgg taaggagttc
     5941 tggcgattag ctggccgtta atcgcctctt ttcagtgcat tctcgggata tccgtgcccc
     6001 atctcccatt ttcccttacc cgtaccgtca ccgccttgtc atctttctga caccttacta
     6061 tcttacaaat gtaacaaaaa agttattttt ctgtaattcg agcatgtcat gttaccccgc
     6121 gagcataaaa cgcgtgaatt cgcgcattcg gtacaacaag agagataaac gatgaaaccg
     6181 ttacattata cagcttcagc actggcgctc ggactggcgt taatggggaa tgcacaggca
     6241 gtgacaacca ttccgttctg gcattctatg gaaggggaac tgggtaaaga ggtggattct
     6301 ctggcccaac gttttaacgc cgaaaacccg gattacaaaa ttgtaccgac ctataaaggc
     6361 aactacgaac agaatttaag cgcggggatt gccgcatttc gtaccggcaa cgctccggct
     6421 attttgcagg tttatgaagt tggcaccgcc accatgatgg cgtcgaaagc cattaaaccg
     6481 gtgtatgacg tgtttaaaga ggcggggatt caattcgatg agtcgcagtt tgtgccgacg
     6541 gtttcaggtt actattccga cagcaaaacc ggccacttac tctcccagcc gttcaacagc
     6601 tcgacccccg ttctctatta caacaaagac gccttcaaga aagcaggatt agacccggaa
     6661 cagccgccga aaacctggca ggatctggcg gactattctg caaaactgaa agcctccggc
     6721 atgaagtgcg gctacgccag cggctggcag ggctggatcc aactggaaaa ctttagcgcc
     6781 tggaacggtc tgccgtttgc cagcaaaaac aacggctttg acggcacgga tgcggtgctg
     6841 gagttcaaca agccggagca ggtgaaacac atcgccatgc tcgaagagat gaacaagaag
     6901 ggcgatttca gctacgtcgg gcgtaaggat gaatccaccg agaagttcta taacggtgat
     6961 tgcgcgatga ccaccgcctc ttccggttct ctcgctaata ttcgcgagta cgccaaattc
     7021 aactatggcg taggcatgat gccttacgac gccgatgcga aagatgcacc gcaaaacgcc
     7081 attatcggcg gggccagcct gtgggtgatg cagggcaaag ataaagaaac ttataccggc
     7141 gtggcgaagt tcctcgactt cctcgctaag ccagaaaatg ctgccgagtg gcatcagaaa
     7201 actggttatc tgccaatcac caaagcggcg tatgacttga ctcgtgagca gggcttttat
     7261 gagaaaaacc caggggcgga taccgcgacg cgtcagatgc tgaataagcc gccgttgccg
     7321 ttcaccaaag ggctgcgtct gggcaacatg ccgcagatcc gcgtgattgt ggatgaagag
     7381 ctcgagagcg tgtggaccgg taagaagaca ccacagcagg cactggatac cgccgttgag
     7441 cgtggaaatc agttgctgcg ccgctttgag aaatcgacga agtcttaatc agtgtaatgt
     7501 cggatgcgtt tcgcttatct gacctggcat cgcgtgtagg ccggataagc gaagcgcatc
     7561 cggcacagtt caggaattaa ccgtaatgtc atcatcccgt ccggtgttcc gctcgcgctg
     7621 gctgccttat ctgctggtcg cgccgcagct catcatcacc gttatctttt ttatctggcc
     7681 tgcgggcgaa gcgttgtggt actcgctgca aagcgtcgat ccgtttggtt tctccagcca
     7741 gtttgtcggc ctggataact tcgtcgcgct gtttcatgac agctactatc tcgactcctt
     7801 ctggacgacg ataaaattca gcacctttgt caccgtcagc ggtttgctgg tatcgctgtt
     7861 ctttgcggcg ctggtggagt acatcgtgcg cggcagtcgt ttctatcaaa ccttgatgct
     7921 gctgccgtat gccgtggctc ccgccgttgc cgccgtattg tggatcttcc tgtttaaccc
     7981 cggtcgcggg ctgatcaccc attttctcgc tgagttcggc tacgactgga accacgcgca
     8041 aaacagcggt caggcaatgt ttctggtggt gtttgcctca gtatggaagc aaatcagcta
     8101 caacttcctg ttcttctatg ccgcgctgca atccattccc cgttcgttga tcgaagccgc
     8161 agccatcgac ggtgcagggc cgattcgccg cttctttaag attgcgctgc cgcttatcgc
     8221 cccggtgagt ttcttcctgc tggtagtgaa cctggtgtat gccttcttcg acaccttccc
     8281 ggtgatcgac gccgccacgt ccggcgggcc ggttcaggcc accacgacgc tgatttataa
     8341 gatctaccgc gaaggcttta ctggactgga tctggcttcg tctgccgcac agtcggtggt
     8401 gttgatgttc ctcgtcatcg tgctgacggt ggtgcagttc cgctatgttg aaagcaaggt
     8461 gcgttaccaa tgattgagaa ccgtccgtgg ctgacgatat tcagccatac catgctgatc
     8521 ctcgggatcg cggtgatcct cttcccgctg tacgtggcgt ttgtcgcggc gacgctggat
     8581 aaacaggccg tctacgccgc gccgatgacg ctcatccccg gcacgcatct gctggaaaac
     8641 atccacaaca tctgggtaaa cggggtaggc acaaatagtg cgccgttctg gcggatgttg
     8701 cttaacagct tcgtgatggc gttcagcatt acgctcggca aaattaccgt ctcgatgctc
     8761 tcggcattcg ccattgtctg gtttcgtttt ccgctgcgta acctcttttt ctggatgatt
     8821 tttatcaccc tgatgttgcc ggttgaagta cgtatcttcc cgacggtgga agtcatcgcc
     8881 aacctgaaga tgctcgacag ctacgccggt ttaacgctgc cgctgatggc ctcggcgacc
     8941 gcgacgtttc ttttccgcca gttctttatg accctaccgg atgaactggt ggaagccgcg
     9001 cggatcgacg gcgcgtcgcc aatgcgcttc ttttgcgaca tcgtttttcc gttgtccaaa
     9061 actaatctgg cggcgttgtt tgtgatcacc tttatttacg gctggaacca gtatttgtgg
     9121 ccgttgttaa ttattaccga cgtggatctc ggcaccaccg tggcagggat caaagggatg
     9181 atcgccactg gcgaaggcac cacagaatgg aactcagtga tggcggcgat gttgttaacg
     9241 cttatccctc cggtggtgat tgttttagtg atgcagcgtg cctttgtgcg cggcctggtc
     9301 gatagtgaga aataagatgg caggactgaa attacaggca gtaaccaaaa gctgggatgg
     9361 caaaacccag gtgattaaac cgctgaccct tgatgtggcg gatggcgaat ttatcgtgat
     9421 ggtcgggccg tctggctgcg ggaaatcgac gctgctgcgt atggttgccg ggctggagcg
     9481 ggtgacggaa ggcgatatct ggatcaacaa tcagcgggtg accgaaatgg agccgaaaga
     9541 tcgcgggatt gcgatggtgt tccagaacta cgcgctttat ccgcatatga gcgtcgaaga
     9601 aaacatggcg tgggggctga aaattcgcgg catgggcaag cagcaaattg ccgagcgcgt
     9661 taaagaagca gcgcgcattc tggaactgga cggtctgctt aagcgccgcc cgcgcgagct
     9721 ttccggcggt cagcgtcagc gtgtggcgat gggccgcgcg attgtgcgcg atccggcggt
     9781 gttcctgttt gatgagccac tctctaacct cgatgccaag ctgcgcgtac agatgcgtct
     9841 tgaactgcaa cagctgcacc gtcgcctgaa aacgacatca ctctacgtta ctcacgatca
     9901 ggttgaggcg atgaccctcg cccagcgagt aatggtgatg aacggcggcg ttgccgaaca
     9961 gattggcaca ccagtagaag tctacgaaaa gcccgccagc ctgtttgtgg cgagttttat
    10021 tggcagcccg gcgatgaatc tgctgacagg ccgcgtgaat aacgaaggca cgcatttcga
    10081 actggacggc ggtattgagc tgccgctaaa cggtggctac cgtcagtatg ccgggcgtaa
    10141 aatgaccctc ggcattcgcc cggaacatat tgcgctaagc tcgcaggcag aaggtggcgt
    10201 accgctggtg atgtatacgc tggagatcct cggcgcagat aacctggcgc acggacgctg
    10261 gggcgaacag aagctggtgg tacggctggc gcatcaggag cgcccgacgg caggcagcac
    10321 gctgtggctg catctgccgg aaaatcagct acatcttttt gatggtgaaa caggacaacg
    10381 agtatgagta actggcctta tccccgcatc gtcgcccacc gtggcggcgg taaactggcc
    10441 ccggaaaaca ccctggcggc aatcgacgtc ggggcaagat acggtcataa gatgatcgaa
    10501 tttgacgcga agttatcgaa agatggcgag atcttcctgc ttcatgacga caatctcgaa
    10561 cgtaccagta acggttgggg cgtcgcgggt gaactgaact ggcaggattt actgcgcgtg
    10621 gatgcaggca gttggtacag caaagcgttt aaaggtgagc cgctgccgtt gctttcgcag
    10681 gtggcggaac gctgtcgcga acacgggatg atggcgaata tcgaaatcaa acccaccact
    10741 ggcaccgggt cgttgacggg caaaatggtg gcgctggcgg cacgcgaact gtgggccggt
    10801 atgacgccac cgctgctgtc atcgtttgag attgatgctt tagaagctgc acaacgggcg
    10861 gcaccggaat tgccgcgcgg tttgttgctg gatgaatggc gcgacgactg gcgcgaactg
    10921 accgcgcggc tgggctgcgt ctctattcat ctcaatcata agttactcga taaagcgcga
    10981 gtgatgcagt tgaaagacgc cggattacgg attctggttt ataccgtcaa taaaccccag
    11041 cgcgcggcag agttgctgcg ctggggcgtg gattgcatct gtactgatgc gattgatgtt
    11101 attggtccga actttacggc ccaatagttt tcagcggaat gtcaggctgc ggcgtgctgg
    11161 gtggattcag catgtcgccg tttttcctct gttgcagcat atgctgctga tttagcgaac
    11221 tatccggatt atggttgttg tttaacatcc cgccgttggt gttgggcagc atttgctgcc
    11281 gggcggggtt tag
//
LOCUS       DAZUCE010000165        11270 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZUCE010000165 DAZUCE010000000
VERSION     DAZUCE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.165"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            125..265
                     /locus_tag="ACHY03_003662"
                     /pseudo
     CDS             125..265
                     /locus_tag="ACHY03_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(290..604)
                     /gene="fliE"
                     /locus_tag="ACHY03_003663"
     CDS             complement(290..604)
                     /gene="fliE"
                     /locus_tag="ACHY03_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFS3906846.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            819..2477
                     /gene="fliF"
                     /locus_tag="ACHY03_003664"
     CDS             819..2477
                     /gene="fliF"
                     /locus_tag="ACHY03_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFS3906847.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAQAVKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2470..3465
                     /gene="fliG"
                     /locus_tag="ACHY03_003665"
     CDS             2470..3465
                     /gene="fliG"
                     /locus_tag="ACHY03_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFS3906848.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANHGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3458..4144
                     /gene="fliH"
                     /locus_tag="ACHY03_003666"
     CDS             3458..4144
                     /gene="fliH"
                     /locus_tag="ACHY03_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFS3906849.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4144..5517
                     /gene="fliI"
                     /locus_tag="ACHY03_003667"
     CDS             4144..5517
                     /gene="fliI"
                     /locus_tag="ACHY03_003667"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFS3906850.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5536..5979
                     /gene="fliJ"
                     /locus_tag="ACHY03_003668"
     CDS             5536..5979
                     /gene="fliJ"
                     /locus_tag="ACHY03_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFS3906851.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            5976..7103
                     /gene="fliK"
                     /locus_tag="ACHY03_003669"
     CDS             5976..7103
                     /gene="fliK"
                     /locus_tag="ACHY03_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFS3906852.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KADPQLLVATDKPSAKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRIANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7208..7672
                     /gene="fliL"
                     /locus_tag="ACHY03_003670"
     CDS             7208..7672
                     /gene="fliL"
                     /locus_tag="ACHY03_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFS3906853.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7677..8681
                     /gene="fliM"
                     /locus_tag="ACHY03_003671"
     CDS             7677..8681
                     /gene="fliM"
                     /locus_tag="ACHY03_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFS3906854.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8678..9091
                     /gene="fliN"
                     /locus_tag="ACHY03_003672"
     CDS             8678..9091
                     /gene="fliN"
                     /locus_tag="ACHY03_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFS3906855.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTNSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9094..9459
                     /gene="fliO"
                     /locus_tag="ACHY03_003673"
     CDS             9094..9459
                     /gene="fliO"
                     /locus_tag="ACHY03_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFS3906856.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVDDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9459..10196
                     /gene="fliP"
                     /locus_tag="ACHY03_003674"
     CDS             9459..10196
                     /gene="fliP"
                     /locus_tag="ACHY03_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFS3906857.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10206..10475
                     /gene="fliQ"
                     /locus_tag="ACHY03_003675"
     CDS             10206..10475
                     /gene="fliQ"
                     /locus_tag="ACHY03_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFS3906858.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10484..11269
                     /gene="fliR"
                     /locus_tag="ACHY03_003676"
     CDS             10484..11269
                     /gene="fliR"
                     /locus_tag="ACHY03_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFS3906859.1"
                     /translation="MMQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
BASE COUNT         2661 a         3005 c         3091 g         2513 t
ORIGIN      
        1 cactatcaaa aatcgctcat atccatagcc cggaatatga ataaaaacat caacaaagat
       61 taaattatcc agaggttact gttgcgtctg tggtcgtaac aacatccgac gtggtgatgt
      121 gtgtcaaatg agacattctg atgatcagag ggtttatcgt gtatacggat cgtggatggc
      181 agaaaacttt caggatcagg tatccatttc aaaccagata atgagtaagt ttgccccatc
      241 catgccccac gcggtgagat cggatgtcat taacaacagg ttacacaacc tatacctgca
      301 tgctcattac ttcctgatat gccgccacca gcttattacg cacctgaatc cccatttgca
      361 ttgaaactga ggctttttgc atatcggtca tcacatcgtt taacgccacg ccgggttcac
      421 cgagagtgaa tttttctgcc tgcgtgcggg cggctgtttg tgtatcgctt atgcgatcga
      481 gcgcggcgtg cagctgcccg gcaaaactaa tggtcggttg cggcagtgat tcctgcgcac
      541 gcgcactcat cgccgtagcc tgtaactggc tgataacccc ttcaatcccc tgtatcgctg
      601 acattttcat ctcctggata atttctggta gcaaagccta ccagtaagtc aataagacaa
      661 aggcgctaaa tagcaacaaa aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca
      721 aataatggga gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt gttccacttt
      781 gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact gcagcccaga
      841 caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg
      901 ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact
      961 accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaatcgtc agccaactga
     1021 cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgaa gttccggcag
     1081 ataaagttca cgagctgcgt ctgcgcctgg cgcaacaagg tttgcccaaa ggcggcgcgg
     1141 tcggtttcga actgctcgat caggaaaagt ttggtatcag ccagttcagc gaacaggtga
     1201 attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc ggcccggtaa
     1261 aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat
     1321 ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat gaagggcaaa
     1381 ttagcgccat tgtgcatctg gtttccagcg ccgttgctgg tctgccgccg ggaaacgtca
     1441 cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg cgcgatctta
     1501 atgacgctca attgaaatat gccagcgatg tcgaaggccg tattcagcgg cgtattgaag
     1561 cgatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggttacg gcgcagctgg
     1621 acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat gaatctcagg
     1681 cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccgggcg
     1741 gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc
     1801 ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat agcgggccgc
     1861 gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca
     1921 aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct
     1981 tgccagatgg caaaccgttg cctctcagca acgaacagat gaagcaaatt gaagatctga
     2041 cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc gtcaactcgc
     2101 cgttcaatag cagtgacgaa agcggcggag aactgccatt ctggcaacag caagcgttta
     2161 tcgatcagtt acttgctgcc ggtcgctggt tgctggtact gctagtggcg tggctgctgt
     2221 ggcggaaagc ggtacgcccg cagctaacac gtcgcgccca ggcggtgaaa gctgtgcagc
     2281 aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg agcaaagacg
     2341 aacaactaca acaacggcgc gctaaccaac gactgggtgc agaagtcatg agccagcgta
     2401 tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc cagtggataa
     2461 ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcttgct gatgaccatt
     2521 ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaggt gcaaaccctg
     2581 agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccga tgtgctggcg
     2641 gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa cgattatctg
     2701 cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct ggaagatatt
     2761 ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga gccgcagagc
     2821 gccgccgatc tgattcgtga tgagcatccg caaattatcg ccaccattct ggtgcatctg
     2881 aagcgcgccc aggccgccga tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg
     2941 atgttgcgta tcgccacctt tggcggtgtg cagccagccg cgctggcgga actgaccgaa
     3001 gtactgaatg gtttgctcga cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga
     3061 acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt tattaccgcc
     3121 gtgcgtgaat tcgacggcga gctggcgcag aaaatcatcg acgagatgtt cctgttcgag
     3181 aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa
     3241 tcgctgttga tcgcgctaaa aggggccgag cagccactgc gcgagaaatt cctgcgcaat
     3301 atgtcgcagc gtgccgctga tattctgcgc gacgatctcg ccaaccatgg tccggtgcgt
     3361 ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg ccttgccgaa
     3421 actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc tgccgtggaa
     3481 aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca tggtcgagcc
     3541 ggaagaaacc atcattgaag aggccgaacc cagccttgag cagcaactgg cgcaactgca
     3601 aatgcaggcc catgagcaag gttatcaggc gggtattgcc gaaggtcgcc agcaaggtca
     3661 taagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg cagaggcgaa
     3721 gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat ttcaaactac
     3781 ccttgatgca cttgatagtg tgatagcgtc gcgcctgatg cagatggcgc tggaggcggc
     3841 acgtcaggtc atcggtcaga cgccaacagt ggataactcg gcactgatca aacagatcca
     3901 acagctgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg tgcacccgga
     3961 tgatctgcaa cgtgtggatg atatgctcgg cgctacctta agtttgcatg gctggcgctt
     4021 gcggggcgat cccaccctcc atcctggcgg ttgcaaagtc tccgccgatg aaggtgatct
     4081 cgacgccagt gtcgccaccc gctggcaaga actctgccgt ctggcagcac caggagtggt
     4141 gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga agccaaaatg
     4201 gcgcagttgc ctgcggtacg tcgctacggg cgattaactc gcgctaccgg gctggtgttg
     4261 gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgagcg ccagaacggc
     4321 agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg gctgttttta
     4381 atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gtgtttatgc caaaaacatt
     4441 tcggcagaag ggctacaaag cggcaagcag ttgccgctcg gtccggcgtt attaggtcgc
     4501 gttctggacg gcagcggtaa acccctcgat ggcctgccct cccccgatac gacggaaacc
     4561 ggtgcgctga ttaccccacc atttaaccca ttgcaacgta caccgattga acatgtgctg
     4621 gacaccggcg tgcgcccaat caatgccctg cttaccgtcg ggcgcgggca gcgtatgggg
     4681 ctgtttgctg gttctggtgt cggtaaaagt gtgctgctgg ggatgatggc gcgttacacc
     4741 cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt aaaagatttt
     4801 attgagaata tcctcggtgc cgaagggcgt gcacgatcag ttgtgattgc cgctccggcg
     4861 gatgtttctc cgctcctgcg aatgcagggg gccgcctatg ccacgcgcat tgccgaagat
     4921 tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg ctacgcgatg
     4981 gcccagcgtg agattgcact agccattggc gaaccgcctg ccaccaaagg ctacccaccg
     5041 tcagtattcg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat tagcggcggc
     5101 ggctcgatta ccgcgtttta taccgtgctc actgagggcg atgaccagca ggaccccatt
     5161 gccgactccg cgcgggcaat cctcgacggt cacattgtgc tgtctcgccg actggcggaa
     5221 gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat gacggcgttg
     5281 atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc gagttttcag
     5341 cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc gatgctcgac
     5401 aaagccatcg ccctgtggcc gcagctggag ggctatttgc aacaaggcat ttttgaacgc
     5461 gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt gtcataaccc
     5521 aggagataac ggcagatggc agaacatggc gcgctggcga ccctgaaaga tctggcagaa
     5581 aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg tcagcaggcg
     5641 gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa cctcaacagc
     5701 gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt tatccagacg
     5761 ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca gaaagttgac
     5821 attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca gacactgcag
     5881 gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa aaagatggat
     5941 gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg cccttgatta
     6001 ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct gcacaagatt
     6061 ttctcgcgtt gttgagcgaa gcattagcag gcgagacaac taccgacaaa gcggaccccc
     6121 agttgctggt ggcaacagat aagccctcgg caaaaggcga gccgctggtc agcgagattc
     6181 ttgccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacaccg cctgtcatca
     6241 acgacgaaca atccacatca acaccgttaa ccaccgctca gacgatgacg ttggctgcgg
     6301 tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa gacgtcaccg
     6361 caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc aaagtgactg
     6421 atgtgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa ctgacttctg
     6481 cgcaactcac aacagcacag cctgatgatg cccccggcac accagctcag ccattaacac
     6541 cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca ccggtgaccg
     6601 ctgccgccag cccgctaatc actccacacc agacacagcc actgcccacc gtcgccgcgc
     6661 ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc cagcatattt
     6721 cgctgttcac ccgccagggg caacaaagtg cagagttgcg tctgcacccg caggatttag
     6781 gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag atggtttcac
     6841 cgcatcaaca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt acgcagctgg
     6901 ccgaaagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt agtggtcagc
     6961 agcaggctgc ttcccagcaa cagcaaagcc aacgcatagc aaaccatgaa cctctggcgg
     7021 gggaagacga cgatacgctt ccggttcccg tctctttaca agggcgtgta acaggcaaca
     7081 gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt cttttccccg
     7141 ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca caggaagacc
     7201 gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat
     7261 tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact ggcattcgca
     7321 tcaggttgcc gctgacgaca aagcgcagca acgcgtcgtg ccctcaccgg tcttctatgc
     7381 gctggatacc ttcacggtca atttgggcga tgcggatcgc gtgctttata tcggcataac
     7441 cctgcgcctg aaagatgaag ctacccgctc gcggctgagt gagtatttgc cggaagtccg
     7501 tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga cagaagaagg
     7561 caagaaaaac ctcattgccg agattaaaac cacactttcc accccgcttg ttgccgggca
     7621 accgaaacag gatgtcaccg acgtgctgta taccgctttt attctgcgat aacgacatgg
     7681 gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac agcgaagtca
     7741 aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac gatccgaata
     7801 cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat gagcgctttg
     7861 cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat ataaccgtcg
     7921 gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc
     7981 tgaaccttat ccatctgaaa ccgctgcgcg gcactgggct ggtggtgttc tcaccgagtc
     8041 tggtgtttat cgccgtggat aacctgtttg gcggcgatgg acgcttcccg accaaagtgg
     8101 aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgttg aaactggcgc
     8161 ttgaaggcta tagcgacgca tggaaggcga ttaatccgct ggaagttgag tacgtgcgtt
     8221 cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt gtggttaaca
     8281 cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgc ctgccattca
     8341 gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg
     8401 aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag ctggagctgg
     8461 tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg
     8521 acgtcctgcc gatagaaaaa cccgatcgca tcatcgccca tgttgacggc gtcccggtgc
     8581 tgaccagtca gtacggcacc ctcaacggtc agtatgcgtt acggatagaa catttgatta
     8641 acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga ataatccggc
     8701 cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg aacaaaaatc
     8761 aaccaacagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag
     8821 cggaacgttg caggatatcg acctgattat ggatattccg gtcaagctga ccgtcgagct
     8881 gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt ccgtcgtggc
     8941 gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt taatcgccca
     9001 gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata tcattactcc
     9061 gtctgagcga atgcgccgcc tgagccgtta gtgatgaata accacgctac tgtgcaatct
     9121 tccgcgccgg tttctgctgc gccactgctg caggtgagcg gcgcactcat cgccattatt
     9181 gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc taagcgcaca
     9241 ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga acgggttgtg
     9301 gtggtcgatg tggatgatgc acggctggtg ctcggcgtta ccgcagggca aatcaatctg
     9361 ctgcataaac ttcccccttc tgcaccaacg gaagagatac cgcagaccga ttttcagtcg
     9421 gtcatgaaaa atttgcttaa gcgtagcggg agatcctgat gcgtcgttta ttgtctgtcg
     9481 cacctgtcct tctctggctg attacgcccc tcgccttcgc gcaactgccg ggtatcacca
     9541 gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca
     9601 tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc acccgcatca
     9661 tcattgtttt tggtttattg cgtaacgcgc tgggaacacc ctccgcgcca cctaaccagg
     9721 tattgctggg gctggcactg tttttgacct tttttattat gtcaccggtg atcgacaaaa
     9781 tttatgtcga tgcgtaccag ccattcagtg aagagaaaat atcaatgcag gaggcgctgg
     9841 aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca gacccgtgag gcagatttag
     9901 ggttgtttgc cagactggcg aataccggcc cgttgcaggg acctgaagcc gtgccgatgc
     9961 gcattttgct cccggcctac gtgaccagcg agttgaaaac cgcatttcag ataggcttca
    10021 cgattttcat cccttttttg attatcgacc tggtgatagc cagcgtgttg atggcattgg
    10081 ggatgatgat ggttccccca gccaccattg ctctgccctt taaactgatg ctgtttgtac
    10141 tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac agctagagag
    10201 gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa gtcgcgctgg
    10261 cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc agtattttgc
    10321 aggccgccac gcagattaac gaaatgacgc tgtcgtttat tccaaaaatc atcgccgtat
    10381 ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca
    10441 ccttgttcac taacctgccg tatatcatcg ggtaggccga acaatgatgc aggtgacaag
    10501 cgaacaatgg ctatcctggt taagcctgta cttctggccg ttactgcgcg tgctggcgct
    10561 catttctacc gcacctattc tgagcgaacg cagcgtgccg aaacgggtaa aactggggct
    10621 ggcaatgatg atcacgttcg ccattgcccc atcattacct gccaacgatg ttcctgtttt
    10681 ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg cgcttggttt
    10741 taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat
    10801 ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc ccgttttagc
    10861 gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc atttatggtt
    10921 gatttcactg ctggtcgata cctttcacac cctgccgatt ggtggcgaac cgttgaacag
    10981 caatgcgttt ctggcactca ccaaagcagg gagtttgatt ttccttaacg ggctgatgct
    11041 ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt tactgaatcg
    11101 tatggccccg caattatcca tttttgttat tggatttcca ttaactctga ctgtcggtat
    11161 ctctttaatg gcggcattaa tgccgttaat tgcgcctttt tgcgaacatt tattcagtga
    11221 gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
//
LOCUS       DAZUCE010000166        11176 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZUCE010000166 DAZUCE010000000
VERSION     DAZUCE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.166"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            60..662
                     /gene="lpfA2"
                     /locus_tag="ACHY03_003677"
     CDS             60..662
                     /gene="lpfA2"
                     /locus_tag="ACHY03_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA2"
                     /protein_id="HFS3906860.1"
                     /translation="MKPNMIVGALALTSVFMAGHLQAADGTVHFRGEIIDSTCEVTPE
                     TKDQVVDLGKVNRTAFSGVDDVAAPTAFSIDLTQCPETFKSAAIRFDGNEDAHGNGNL
                     AIGTPLDNSNDAAAGISPSDNSGDYTGAGAVSAAKGVAIRLYNRADNTQVKLYENSAS
                     TPISNGNASMKFMARYIATETTIDPGTANADSQFTVEYIK"
     gene            771..1451
                     /locus_tag="ACHY03_003678"
     CDS             771..1451
                     /locus_tag="ACHY03_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFS3906861.1"
                     /translation="MYLLRLFFAVTLITQMSMAMAGIVVGGTRVIFYGDKPDTTISIF
                     NKETNLPYLLQTWVDPFNKEDKSKPPFSVIPPVSRIEPQQEKILRILKTAGELPKDRE
                     SVFWLNVKNIPPSLDKKNTNVLEIAIKTRIKLFWRPAAVKIIPEQAIKNVKWSRQGNN
                     LIVENPNPIHINVMSVTVDDKDVSLNMIRPFETLTLPIPAGVAGKSLVWRFINDYGAI
                     SQPLKKNL"
     gene            1473..4007
                     /locus_tag="ACHY03_003679"
     CDS             1473..4007
                     /locus_tag="ACHY03_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS3906862.1"
                     /translation="MASSHLFITLASGICLLCSISAFARDSLFNPRLLELDHTADNID
                     IHQFNRSNTLPAGTYKVDVMINGMLFERQEVKFVQDNPDAELHPCYVAIKNVLATYGI
                     KVDAIKSLANVDDKTCVNPVPLIDGATWLLDASKLALNITIPQIYLNNAVNGYISPSR
                     WDQGINAMMMNYDFSASHTIRSNYDDDDDSYYLNLRNGINLGAWRFRNYSTLNSYDGN
                     VDYHSVSNYIQRDIMALRSQIMIGDTWTASDVFDSTQVRGVRLYTDDDMLPSSQNGFA
                     PVVHGIAKTNATVIIKQNGYVIYQSAVPQGAFALTDLNTTSSGGDLDVTIKEEDGSEQ
                     HFIQPFTSLAILKREGQTDVDLSIGEVRDESGFTPEVLQLQAMHGFPLGITLYGGTQL
                     ANDYASAALGIGKDMGALGAISFDVTHARSQFDYGDNESGQSYRFLYSKRFEDTNTTF
                     RLVGYRYSTEGFYTLNEWVSRQDNDSDFWVTGNRRSRFEGTWTQSFTPGWGNIYLTFS
                     RQEYWQTDEVERLLQFGYNNNWRNISWNVSWNYTDSIKRSSGNHHDDNNDDFGKEQIF
                     MFSMSIPLSGWMEDSYVNYSLTQNNHHESTMQVGLNGTMLEGRNLSYNVQESWMHSPD
                     DSYSGNAGMTYDGTYGSVNGSYSWSRDSQHFDYGARGGVLVHSDGVTFSQELGETVAL
                     VKAPGAEGLSIENATGISTDWRGYTVKTQLSPYDENRVALNSDYFSKANIELENTVIN
                     LVPTRGAVVKAEFVTHVGYRVLFNVRQVNGKPIMFGAMATASLETGTVTGIVGDNGEL
                     YLSGMPEKGEFLLSWGQAADEKCKAAYHITHKPDDTSLIQMDAICR"
     gene            4019..5110
                     /locus_tag="ACHY03_003680"
     CDS             4019..5110
                     /locus_tag="ACHY03_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3906863.1"
                     /translation="MKKILSGLILLLCCPYGFAANGDGVCFNSDGATHMSNLSFGPLT
                     VAAANNHSGYNIFEALSNTTGTYPVRCHCDDTHGGPGQQTAFFPIFYTGDAAPGLVLE
                     RTLNGLNYYALNDYLSVGVTIFIINNQYAAIPFEHLSNQSTSPQHTCGAGNNGSTVNL
                     DSGRSAKLSFYVRHSITGTVTIPTTEVAWLYAGMSDHFPKTTPVSKVTIRGQLTAPQN
                     CELTPNQSIDVDFQKINSAEFSSTAGSIIAERKIKTEVTVSCTGMEDVRSTEVVSASM
                     IAANRSADATMIVTSNPDVGIKIFDKNDRPVNVDGGNLPADMGAISRLGKTDGSVTFY
                     SAPASLTGAKPAPDNGFTATATLVIEFTN"
     gene            5138..6220
                     /locus_tag="ACHY03_003681"
     CDS             5138..6220
                     /locus_tag="ACHY03_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3906864.1"
                     /translation="MKKWHYIFCIILFHLGLPCGYAANDGTCATRGGTHTLSLNFPLT
                     TVSAANNVPGNTLIDIANATSSENYSVLCNCDSKHSNGAYHEIYYTADPAPGMVYSTT
                     ASGLAFYYLNEYVDVGTKISVLNAGYTAVPFEHVSNQATTTDHTCQGNKTTAVGVSLK
                     TGADAKISFRIKRSINGTVVIPITDIALLYANISSTTTRGEAIAKVRISGSLTAPQSC
                     QINAGQVIYFDFDTIPASEFSSTAGQAITSRKITKTVSIECTGMGDERTQKVDASFTG
                     TNRSSDDTMVATDNADVGIKIYNKSNAEVSVNNGKLPADMGNTTIFGRKNGSVTFSAA
                     PASFTGARPQPGVFNATATLTIEFVN"
     gene            6432..7472
                     /gene="pstS"
                     /locus_tag="ACHY03_003682"
     CDS             6432..7472
                     /gene="pstS"
                     /locus_tag="ACHY03_003682"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFS3906865.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            7559..8518
                     /gene="pstC"
                     /locus_tag="ACHY03_003683"
     CDS             7559..8518
                     /gene="pstC"
                     /locus_tag="ACHY03_003683"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFS3906866.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            8518..9408
                     /gene="pstA"
                     /locus_tag="ACHY03_003684"
     CDS             8518..9408
                     /gene="pstA"
                     /locus_tag="ACHY03_003684"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFS3906867.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            9499..10272
                     /gene="pstB"
                     /locus_tag="ACHY03_003685"
     CDS             9499..10272
                     /gene="pstB"
                     /locus_tag="ACHY03_003685"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFS3906868.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            10287..11012
                     /gene="phoU"
                     /locus_tag="ACHY03_003686"
     CDS             10287..11012
                     /gene="phoU"
                     /locus_tag="ACHY03_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFS3906869.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         2879 a         2612 c         2808 g         2877 t
ORIGIN      
        1 tccaaattta ataaatggat aacacacagg aataatatca aattaatgga gttatattta
       61 tgaaaccaaa tatgattgta ggagcattag cgttaacttc tgtgtttatg gcaggtcacc
      121 tacaggcggc tgatggaaca gtccatttcc gtggtgaaat tattgacagt acttgcgaag
      181 tcacacctga aactaaagat caggtcgttg acttaggcaa agtaaaccgt acagccttta
      241 gtggcgtcga tgatgtggct gccccgacgg ctttttctat cgatctgact caatgcccgg
      301 aaacctttaa gtccgccgca attcgtttcg atggtaatga agatgctcat ggtaatggca
      361 acctggcaat tggtaccccg ctggataact ctaacgatgc tgccgctggt attagcccga
      421 gtgataacag tggggattat actggtgcgg gtgccgttag tgcagcgaaa ggcgtagcta
      481 ttcgtttata taaccgtgca gataacactc aggtcaagtt atatgaaaat tctgcatcaa
      541 ctccgatttc taatggtaat gcatccatga agttcatggc tcgttatatt gctacggaaa
      601 cgactattga ccctggtaca gctaacgccg actcgcagtt tacagttgaa tatataaaat
      661 aaatcattct taacataata ccttaaataa aatggcgctc gccattatgg ttctctttta
      721 cttaaatgaa gggcgtcatt attatatttt tatgagatac atttatgcct atgtatttac
      781 ttagattgtt ttttgctgtt actttgatta cgcagatgtc gatggctatg gctggtattg
      841 ttgttggggg aactcgcgtt attttttatg gagacaaacc tgataccaca atctctattt
      901 ttaataaaga aacgaacctt ccttatctat tgcagacatg ggttgatcca tttaataaag
      961 aggataaaag caaaccgcca ttttcagtga tacctcctgt ttctcgcatt gaaccacagc
     1021 aagagaaaat attacgcatt ttaaaaacgg ctggcgagtt accaaaagat cgggagtccg
     1081 tattctggtt gaacgtgaaa aacatccctc cttcacttga taaaaaaaat accaatgttc
     1141 ttgagatagc cataaaaaca agaattaagt tattctggcg accagccgcg gtaaagataa
     1201 tccctgagca ggctattaaa aatgttaaat ggtcgcggca gggtaataat ctcatcgtcg
     1261 aaaatccaaa tccgatccat attaatgtaa tgagtgtgac ggtggatgat aaagacgtgt
     1321 cgctaaatat gatccgccct tttgaaacat tgacattacc aattccggct ggcgtggcag
     1381 gaaaatcgct cgtttggcgc tttattaatg attacggtgc aattagtcaa ccactaaaga
     1441 aaaaccttta agcgccggat aaggatatat cgatggcctc ttcgcatctt ttcattacgc
     1501 ttgcatcggg catatgtctg ctctgttcca tatctgcttt tgcccgggat agcttgttca
     1561 accccagatt actggaactg gatcatactg cggataatat tgatattcac cagttcaacc
     1621 gttcgaatac cttacctgcg ggaacataca aagttgatgt gatgatcaac ggcatgctct
     1681 tcgaacgcca ggaagttaaa tttgtccagg ataaccctga tgctgaactc cacccatgct
     1741 acgtggcgat aaaaaacgta ctggcgacct atggtataaa agttgatgcg ataaaatctc
     1801 tggcgaatgt tgatgacaaa acatgcgtaa atcccgttcc gctgatcgac ggggcgacct
     1861 ggttactgga cgccagtaag ctggcattga atattactat tccgcaaatt tatctcaaca
     1921 atgcagttaa tggttatatc agcccttccc gttgggatca ggggatcaat gccatgatga
     1981 tgaattatga tttttcggca tcgcatacca tccggtcaaa ttatgacgac gacgatgaca
     2041 gttattatct gaatttgcgt aatggtatta atttaggcgc atggcgtttt cgtaattaca
     2101 gcaccctgaa ttcttatgac ggtaatgtgg actaccattc cgtcagtaat tacattcagc
     2161 gcgacattat ggcattacgt agccagatta tgattggcga tacctggacg gcaagcgatg
     2221 tatttgatag tacacaggtg cgtggcgtgc ggctgtatac cgatgacgat atgttgccct
     2281 ccagccagaa cggctttgcg ccagtggtac atgggattgc gaaaactaac gccacggtga
     2341 tcatcaaaca aaacggctac gttatttatc aatcagccgt accacagggc gcatttgccc
     2401 tcaccgactt aaacacgacc agtagcggcg gcgatctcga tgtcactatc aaagaagaag
     2461 atggcagcga gcagcacttt attcagccat ttacttcact ggccattctc aagcgtgaag
     2521 gtcagaccga tgtagacctt agcattggag aagtgcgcga cgaaagcggc tttacgcctg
     2581 aggtcttgca gttacaagca atgcacggtt tccctttggg aataaccttg tatggcggaa
     2641 cacaactggc aaatgattac gcttctgccg cgctgggtat tggtaaagat atgggggcgc
     2701 tgggcgcgat ttcttttgac gtgactcatg cccgctcgca gtttgactac ggcgataatg
     2761 agagtggtca atcgtatcgt tttctctatt ccaaacgttt tgaagacacc aataccacct
     2821 ttcgtctggt gggttatcgc tactctacgg aggggttcta caccctcaat gaatgggtgt
     2881 cgcgacagga taacgattct gatttctggg taacgggcaa ccgtcgcagc cgcttcgaag
     2941 gcacctggac gcaatctttc acgccaggct ggggcaatat ttatttaaca ttcagtcgac
     3001 aggaatactg gcagaccgat gaggtcgagc gtttattaca gttcggctat aacaacaact
     3061 ggcgaaacat ctcctggaac gtttcctgga actatacgga ctcgatcaag cgctcatcgg
     3121 gcaaccatca tgatgataac aatgatgatt tcggcaaaga acagattttc atgttctcaa
     3181 tgtcgatacc gctatcgggc tggatggaag acagctacgt caactattcg ttaacgcaaa
     3241 acaaccacca tgaaagcacg atgcaggtcg gtctgaacgg aacgatgctg gaagggcgta
     3301 acctgtctta taacgtacag gaatcgtgga tgcactctcc tgatgactcc tacagcggca
     3361 atgccggaat gacctatgac gggacttatg gctcggtcaa tggcagctat tcctggagcc
     3421 gtgactccca acattttgat tatggcgcca gaggcggcgt gctggtgcat agtgacggag
     3481 tgaccttctc gcaggaactg ggcgaaacgg tggcattagt caaagcgccg ggcgcagaag
     3541 gcctgtccat tgaaaacgcc accgggattt ctaccgactg gcgtggttat accgtaaaaa
     3601 cgcagcttag cccatatgac gaaaaccgcg tggcattgaa cagcgactat ttctccaaag
     3661 ccaatattga actggaaaac accgtcatca acctggtacc aacgcgcggt gcggtggtga
     3721 aagccgaatt tgtcacccat gtcggttatc gcgtgctatt taacgtccgc caggtcaacg
     3781 gtaaaccaat aatgtttggc gcgatggcaa ccgcctctct cgaaacgggc acagtcaccg
     3841 ggattgtcgg tgataacggc gaactgtatc tctccgggat gcctgaaaaa ggcgagtttt
     3901 tattgagttg gggacaagct gcggatgaaa aatgtaaggc ggcctatcac atcacccata
     3961 aacctgatga taccagcctg attcaaatgg atgcgatttg tcgctaaagg aagcatgaat
     4021 gaaaaaaata ttatcagggt tgattctgct gctttgctgt ccttatggtt tcgccgctaa
     4081 tggtgatggc gtttgtttta atagtgatgg cgcaacgcac atgtcaaatt tatcatttgg
     4141 tccgctgacg gtggctgcgg cgaataacca ctccggatac aatattttcg aggcactgag
     4201 caacacgact ggaacatacc cggtgcgctg tcactgtgat gacacgcatg gcggaccggg
     4261 ccaacaaaca gcattttttc ctatcttcta cacgggggat gccgcaccgg ggcttgtgct
     4321 tgagcgcact cttaatgggt taaattacta tgctctgaat gattatttat cggtcggcgt
     4381 gacgattttt attattaata accaatatgc ggccattcct tttgaacact tatccaacca
     4441 atccacctca ccgcaacata cctgtggagc aggtaataat gggagcactg taaatctgga
     4501 ttcagggcgc tcggcaaaat tatctttcta tgttcggcat tctattactg gcacggtgac
     4561 aatacccaca acggaagtcg cctggttgta cgcgggcatg tccgatcatt ttcccaaaac
     4621 gacccccgtt tctaaagtga caattcgcgg acaactaacg gccccgcaga actgtgagtt
     4681 aacgccaaat cagagcatcg atgtcgattt tcaaaaaatt aatagcgctg agttctcctc
     4741 aacggcgggt tcaattattg ccgaaagaaa gatcaaaacc gaagtgactg tatcctgtac
     4801 cgggatggaa gacgtaaggt ccacggaggt ggtgagtgcg tcgatgattg cggcaaacag
     4861 aagtgccgat gccactatga tcgtgacgag taatccggat gtgggaatta agatttttga
     4921 taagaacgac cgtccagtga atgtggatgg gggcaactta cccgctgata tgggtgctat
     4981 tagtcgatta ggaaaaaccg atggtagcgt aacgttttat tcagcgcccg ccagtctgac
     5041 gggagcaaaa ccagcgcctg ataatggatt taccgctaca gccacgctgg ttattgaatt
     5101 tactaactaa tcttgtgtta gtaaaaggaa taatttcatg aaaaaatggc attatatatt
     5161 ttgcataatt ctctttcatt tagggttgcc gtgcgggtat gcggcaaatg atggcacgtg
     5221 tgcaacaaga ggcggcacac atacattaag ccttaatttt cctctgacaa cggtcagtgc
     5281 agcaaacaat gtgcctggaa atacattaat agatattgct aatgcaacat cttctgaaaa
     5341 ttatagcgtt ctgtgtaact gtgattcaaa acatagcaat ggcgcttatc acgaaatata
     5401 ttataccgca gaccctgctc ccggtatggt ttatagcacc accgcaagtg gtcttgcttt
     5461 ttactatctt aacgaatatg tcgatgtggg aacaaaaata tctgtgctaa atgcggggta
     5521 tacggcagtt ccttttgaac atgtttccaa ccaggcaact acaacagatc acacttgtca
     5581 gggaaacaaa actacagcgg ttggcgtgag cctgaaaact ggagcagatg cgaagatttc
     5641 atttcgtatt aaacgttcaa taaatggaac ggtagtaata cctatcaccg atattgcatt
     5701 gctgtatgcc aacatatcca gcaccacgac ccgtggtgag gcgattgcaa aagttcgaat
     5761 ttcaggcagt ttgaccgcac cacagtcttg tcagataaat gcaggacagg tgatttattt
     5821 tgattttgat actattcctg cgtccgaatt ttcatctacc gccgggcaag ccattacttc
     5881 acgaaaaatc actaaaacag tgagtattga gtgtacgggg atgggggatg agcgtacgca
     5941 gaaagtcgat gcttctttta cggggacgaa ccgaagcagt gacgatacga tggttgcgac
     6001 agacaatgcc gatgtcggga tcaaaattta caataaatcg aatgctgaag ttagcgtcaa
     6061 caacggaaag ttacccgcag acatgggcaa cacgaccatt tttggtcgta aaaatggttc
     6121 ggtaactttt tcggcagcac ctgccagctt taccggtgcc cggcctcagc ccggcgtttt
     6181 taacgctacc gcgaccttaa ccattgaatt tgtaaactaa ccttttgcgg ctgttttatg
     6241 acagccgttc atatttcctc tccttttatg aaaaaacgct gatattttta ccctttaata
     6301 tcaataagta ataactttcg tttgggtgtc atcaaactgt cacattcctt acatataact
     6361 gtcacctgtt tgtcctattt tgcttctcgt agccaacaaa caatgcttta tgaatcctcc
     6421 caggagacat tatgaaagtt atgcgtacca ccgtcgcaac tgttgtcgcc gcgaccttat
     6481 cgatgagtgc tttctctgtg tttgcagaag caagcctgac aggtgcaggt gcaaccttcc
     6541 ctgcgccggt gtatgccaaa tgggctgaca cttaccagaa agaaaccggt aataaagtta
     6601 actaccaggg tatcggttct tccggtggcg taaaacagat tatcgctaat accgttgatt
     6661 ttggtgcctc tgacgcgccg ctgtctgatg aaaaattggc tcaggaaggc ctgttccagt
     6721 tcccgaccgt gattggcggc gtggtgctgg cggttaacat tccagggttg aagtctggcg
     6781 agctggtgct ggatggtaaa accctcggcg acatctacct cggcaaaatc aagaagtggg
     6841 atgatgaagc catcgccaaa ctgaatccgg gtctgaaact gccttcacag aacattgctg
     6901 tagtacgccg cgcagatggc tccgggactt ccttcgtctt caccagctac ctggcgaaag
     6961 tgaatgaaga gtggaaaaac aacgttggta ctggctctac cgtaaaatgg ccgatcggtc
     7021 tgggtggtaa aggtaacgac ggtatcgctg cgttcgttca gcgtctgccg ggtgcaattg
     7081 gttacgttga atatgcttat gcgaagcaaa acaacctggc gtacaccaaa ctgatctccg
     7141 ctgatggtaa acctgttttg ccgactgaag aaaacttcgc taatgcagca aaaggtgcag
     7201 actggagcaa aaccttcgct caggatctga ccaaccagaa aggcgaagat gcgtggccta
     7261 ttacctctac cacgttcatt ctgatccaca aagatcagaa gaaaccagaa caaggcacag
     7321 aagtgctgaa attcttcgac tgggcgtaca aaaccggggc taaacaggcg aacgacctgg
     7381 attatgccag cctgccggat agcgtagttg aacaggttcg cgcagcgtgg aagaccaata
     7441 ttaaagacag tagcggcaag ccgctgtact aataaaactc caggccgggt acggtgtttt
     7501 acgccgcatc cggcattaca aaatgacttt gtaaacgcgt ttaactgaag agtaacttat
     7561 ggctgcaacc aagcctgctt ttaacccacc gggtaaaaag ggcgacataa ttttcagcgt
     7621 gctggtaaaa ctggcggcgc tgattgtgct attgatgttg ggtggcatta ttgtctctct
     7681 gatcatctcc tcatggccga gcattcagaa atttggtctg gctttcctgt ggaccaaaga
     7741 gtgggatgcg ccgaacgata tctacggggc gctggtgccg atctacggta cgttggtgac
     7801 ttcgtttatc gcgctgctga tcgccgtccc ggtgagtttc ggtatcgccc tgttcctgac
     7861 tgagcttgcg cctggctggc tgaaacgccc gctgggtatc gccattgagc tgctggcagc
     7921 cattccaagt atcgtttacg gcatgtgggg cctgtttatc tttgcgccgc tgttcgccgt
     7981 ttacttccag gagccggtcg gcaatatcat gtcgaatatc ccgattgtcg gcgcgctgtt
     8041 ctctggcccg gcatttggta tcggtatcct cgcggcaggc gtgatcctcg ccatcatgat
     8101 tattccgtac attgcggcgg taatgcgcga tgtgttcgaa caaaccccgg tgatgatgaa
     8161 agagtcggcc tacggtattg gctgcaccac ctgggaggtt atctggcgta tcgttcttcc
     8221 gttcaccaaa aatggtgtta tcggcggcat tatgctgggg ctgggccgcg cgcttggtga
     8281 aaccatggcg gtgaccttta tcatcggtaa cacctaccag ctcgacagcg cctcgctgta
     8341 catgccgggc aacagtatca cctctgcgct ggcgaacgaa tttgccgaag cggaatccgg
     8401 tctgcacgtt gccgcactga tggaactggg cctgatcctg tttgtgatta ccttcatcgt
     8461 cctcgccgca tcgaagttta tgattatgcg cctggctaag aatgaggggg cacgctaatg
     8521 gctatggttg aaatgcaaac cactgcggcg ctggcggaat cccgccgcaa aatgcaggcg
     8581 cgtcgccgcc tcaagaaccg tattgcactg acgctctcga tggcgacgat ggccttcggt
     8641 ctgttctggc tgatctggat tttaatgtcc accatcaccc gcggtatcga cggtatgtcg
     8701 ctggctctgt ttactgaaat gacgccgccg cccaatacgg aaggtggtgg tctggcgaac
     8761 gctctggcgg gtagcgggct gttaattttg tgggccacgg tattcggtac gccgctgggc
     8821 attatggcgg ggatttatct ggcggaatat ggtcgtaaat cctggctggc agaagtgatt
     8881 cgcttcatta acgacattct gctctctgcg ccgtcgattg tggttggtct gtttgtttac
     8941 accattgtgg tggcgcagat ggagcacttc tccggttggg cgggtgtgat tgccctggcg
     9001 ctgttgcagg taccgattgt tatccgcacc accgagaaca tgctgaaact ggtgccggac
     9061 agcctgcgtg aagcggctta tgcgctgggt acaccgaagt ggaagatgat ctccgcgatt
     9121 acgctgaaag cgtcggtgtc cgggatcatg accggtatcc tgctggcgat tgcccgtatt
     9181 gctggtgaaa ccgcgccgct gctgtttacc gcgctctcca accagttctg gagcacggac
     9241 atgatgcagc cgatcgccaa cctgccggtg acgatcttta agtttgcgat gagcccgttt
     9301 gctgaatggc agcaactggc ctgggccggg gtgttgatta ttaccctgtg cgtactgctg
     9361 ctgaacattc tggcgcgcgt tgtttttgcg aagaataaac acggttaaca ctttgccgga
     9421 tgcggcgtga acgcctgatc cggcctacgg taagcctgat tagcgaagtg catcaggcac
     9481 gatgaggaaa agattgcaat gagtatggtt gaaactgccc cgagtaaaat tcaggttcgt
     9541 aatttgaact tctactacgg caaattccat gccctgaaaa acatcaacct ggatatcgct
     9601 aaaaaccagg ttacggcgtt tatcgggccg tccggctgcg gtaaatcgac gctgctgcgt
     9661 accttcaaca aaatgtttga actgtacccg gagcagcgcg cagaaggtga aattctgctt
     9721 gatggcgaca acatcctgac caactctcag gatatcgccc tgctgcgtgc gaaagtgggc
     9781 atggtgttcc agaaaccgac gccgttcccg atgtccatct acgacaacat cgcctttggc
     9841 gttcgtctgt ttgagaagct ctcccgtgcc gacatggacg agcgcgtgca gtgggcattg
     9901 accaaagccg cattgtggaa cgaaaccaaa gataaattgc atcagagcgg ttactctctc
     9961 tctggcggtc agcaacagcg tctgtgtatt gcgcgtggta tcgccattcg cccggaagtg
    10021 ctgctgctcg acgaaccgtg ttcggcgctc gaccctatct ctaccgggcg tattgaagag
    10081 ctgatcaccg aactgaagca ggattacacc gtggtgatcg tcacccacaa catgcagcag
    10141 gctgcgcgtt gttccgacca cacggcgttt atgtacctgg gcgaattgat tgagttcagc
    10201 aacacggacg atctgttcac caagccagcg aagaaacaaa cagaagacta catcaccggt
    10261 cgttacggtt gattcaggag tgcgttatgg acagtctcaa tcttaataaa catatttccg
    10321 gccagttcaa cgccgaactg gaaagtatcc gcacgcaggt gatgaccatg ggcggcatgg
    10381 tggagcagca gctttctgat gcaatcaccg cgatgcacaa ccaggacagc gatctggcga
    10441 agcgcgtcat cgaaggcgac aagaacgtca acatgatgga agtggcgatc gatgaagcct
    10501 gcgtgcgcat tattgccaaa cgccagccga cggcgagcga cctgcgactg gttatggtga
    10561 tcagtaaaac cattgccgag ctggagcgta ttggtgacgt ggcggacaaa atctgccgta
    10621 ctgcgctgga gaaattctcc cagcagcatc agccgttgct ggtaagtctg gagtcgctgg
    10681 gccgtcatac catccagatg ctgcacgacg tgctggacgc gttcgcgcgg atggacattg
    10741 acgaagcggt acgtatttat cgtgaagata aaaaagtcga tcaggagtat gaaggtattg
    10801 ttcgtcaact gatgacctac atgatggaag attcgcgtac cattccgagc gtacttaccg
    10861 cgctattctg tgcgcgttct atcgaacgta ttggcgaccg ctgccagaat atttgtgagt
    10921 ttatcttcta ctacgtgaag gggcaggatt tccgtcatgt aggcggtgat gagctggata
    10981 aactgctggc gggaaaagac ccgaaagagt agtgctaact gtaggccaga taaagctttt
    11041 tgccgcatct ggcaatcagc ccggtcatac atagttaccg ggctgataaa ctttctcact
    11101 gaaatattgc cacacaaact cacctcgaaa ctctccccag tacattgcat atacattatt
    11161 acatttgaat tttttt
//
LOCUS       DAZUCE010000167        11125 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZUCE010000167 DAZUCE010000000
VERSION     DAZUCE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.167"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(207..2036)
                     /gene="glmS"
                     /locus_tag="ACHY03_003687"
     CDS             complement(207..2036)
                     /gene="glmS"
                     /locus_tag="ACHY03_003687"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFS3906870.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2198..3568)
                     /gene="glmU"
                     /locus_tag="ACHY03_003688"
     CDS             complement(2198..3568)
                     /gene="glmU"
                     /locus_tag="ACHY03_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFS3906871.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3921..4340)
                     /locus_tag="ACHY03_003689"
     CDS             complement(3921..4340)
                     /locus_tag="ACHY03_003689"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFS3906872.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4361..5743)
                     /gene="atpD"
                     /locus_tag="ACHY03_003690"
     CDS             complement(4361..5743)
                     /gene="atpD"
                     /locus_tag="ACHY03_003690"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFS3906873.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5770..6633)
                     /gene="atpG"
                     /locus_tag="ACHY03_003691"
     CDS             complement(5770..6633)
                     /gene="atpG"
                     /locus_tag="ACHY03_003691"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFS3906874.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6684..8225)
                     /gene="atpA"
                     /locus_tag="ACHY03_003692"
     CDS             complement(6684..8225)
                     /gene="atpA"
                     /locus_tag="ACHY03_003692"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFS3906875.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8238..8771)
                     /gene="atpH"
                     /locus_tag="ACHY03_003693"
     CDS             complement(8238..8771)
                     /gene="atpH"
                     /locus_tag="ACHY03_003693"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFS3906876.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8786..9256)
                     /gene="atpF"
                     /locus_tag="ACHY03_003694"
     CDS             complement(8786..9256)
                     /gene="atpF"
                     /locus_tag="ACHY03_003694"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFS3906877.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9318..9557)
                     /gene="atpE"
                     /locus_tag="ACHY03_003695"
     CDS             complement(9318..9557)
                     /gene="atpE"
                     /locus_tag="ACHY03_003695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFS3906878.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9604..10419)
                     /gene="atpB"
                     /locus_tag="ACHY03_003696"
     CDS             complement(9604..10419)
                     /gene="atpB"
                     /locus_tag="ACHY03_003696"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFS3906879.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10428..10808)
                     /gene="atpI"
                     /locus_tag="ACHY03_003697"
     CDS             complement(10428..10808)
                     /gene="atpI"
                     /locus_tag="ACHY03_003697"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFS3906880.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2663 a         3017 c         2762 g         2683 t
ORIGIN      
        1 acgtgctatt cattacaaag tacgtcaatt atggcaattg tttgtcaatg attcgaggta
       61 tttaattact actacggtta aaacaagtgt tcaacggtgc cccaaataat agctgaagtt
      121 aattatctgg aatgaggtta gattattaat gctggtaagt aaaagggccg ccaaaaggca
      181 gcccttcgtt cagcaaatga ataatattac tcaaccgtta ccgattttgc caggttacgc
      241 ggctggtcaa cgtcggtgcc tttgatcagc gcgacgtgat aagccagtaa ctgcagcgga
      301 acggtgtaga agatcggtgc aatcacctct tccacatgcg gcatctcgat gatgtgcatg
      361 ttatcgctac ttacaaaacc cgcatcctga tcggcgaaga catacaactg accgccacgc
      421 gcgcgaactt cttcaatgtt ggatttcagt ttttccagca attcgttgtt cggtgcaacg
      481 acgataaccg gcatatcggc atcaatcagc gccagcggac cgtgtttcag ttcacctgca
      541 gcgtaggctt cagcgtgaat gtaagagatc tctttcagct tcaatgcgcc ttccagcgcg
      601 attgggtact gatcgccacg gcccaggaac agcgcgtgat gtttgtcaga gaaatcttct
      661 gccagtgctt caatgcgttt gtcctgagac agcatctgct caatacggct cggcagcgcc
      721 tgcagaccat gcacaatgtc atgttcaatg gaggcatcca gacctttcag gcgagacagc
      781 ttcgccacca gcatcaacag cacagttaac tgagtggtga atgctttagt ggatgccacg
      841 ccgatttccg tacccgcgtt ggtcatcagc gccagatcgg attcgcgcac cagagaagaa
      901 cccggaacgt tacagattgc cagtgaacca aggtaaccca gctctttcga cagacgcagg
      961 cccgccaggg tatctgcggt ttcgccagac tgtgacaagg tgatcatcag gctgttacga
     1021 cgcacggcag atttgcgata gcggaattca gaggcgattt cgacgtcgca cggaatacct
     1081 gccagcgatt caaaccagta gcgggaaacc ataccggagt tataagaagt accacaggcg
     1141 aggatctgaa tatgctcaac cttcgacagc agttcgtcgg cgttcggtcc cagctcgctt
     1201 aaatcaacct gaccgtggct gatgcgcccg gtaagggtgt ttttgatcgc gttcggctgt
     1261 tcgtagatct ctttctgcat gtagtgacgg taaatacctt tatcgcccgc gtcatattgc
     1321 agattggatt cgatatcctg acgttttact tccgcgccag ttttatcgaa gatgtttacc
     1381 gaacggcgag tgatttccgc aatatcgccc tcttcaagga agataaagcg acgggtcacc
     1441 ggcagcagcg ccagctggtc agaagcgata aagttttcac ccatacccag accaatcacc
     1501 agcggactac cagaacgtgc tgccagcagg gtatccgggt gacgggagtc catgatcact
     1561 gtaccgtacg caccacgcag ttgcgggata gcacgcagaa cagcctcacg cagagtaccg
     1621 ccctgtttca gctcccagtt caccagatgg gcaatcactt cggtgtcggt ttcagaaacg
     1681 aaggtatagc cacgcgcttt taactcttcg cgcagcggtt catggttttc gatgatgccg
     1741 ttatgcacca ccacaatgtg ttcagaaaca tgcggatgcg cattcacttc tgaaggttca
     1801 ccgtgggtcg cccagcgagt gtgagcaata ccggtgccgc catgcagagg atgttcttcc
     1861 gctgcctgag ccagcatctg gactttaccg aggcgacgca ggcgggtcat atgaccttct
     1921 gcatcaacaa cggccagacc ggcagagtca tatccgcggt attccagacg acgtaaacct
     1981 tcaagaagga tttctgctac atcacgttgc gcgatcgcgc caacaattcc acacatagtt
     2041 tttgattccg atttatatcg ttgtcggtca acctgtatgc ccgttttttc gggcgccccg
     2101 agccttgtag agagtggggt tatttttata gttactgctt gtgagcggga gattatgtta
     2161 tctcctcatc ccatatgacc ggattacccg gccagaatta ctttttcttg accggacgac
     2221 gccagccttc tttctgagtc tgcggcacac ggctgatagc taatgcattt tcgccgacat
     2281 tacgcgtcac agttgtaccc gcagcaatgg ttgcaccttt gcctactgtt accggggcca
     2341 ccagctgagt gtcggaaccg acaaacacat cgtcgccgat aatggtctta aatttattcg
     2401 caccatcgta gttgcaggta atggttcccg cgccgatgtt aacgttatcg ccaatttccg
     2461 catcgccaag gtaagtcaga tgaccagctt tcgagccttt acccagacgt gcttttttca
     2521 tctcaacaaa gttaccgacg tgtgcacctt cgagcaactc agcaccagga cgcaaacggg
     2581 caaacgggcc aatggtacag gccgccgcca gattcgcgtc ttccacgacg gtatatgggc
     2641 taatttcgca atcatcgcca atcacgctgt ttttaatcac gcaaccggtg ccgattttca
     2701 cgcgatgacc gagagtcacg ttgccctcga tgataacgtt agtatcaatt tcaacatcgc
     2761 gcccgtgagt aagcgtaccg cgcagatcaa aacgcgccgg atcgcgcagc ataacgcctg
     2821 ctaacagcag tttttcagcc tgttcggact ggtaaacgcg ctccagacgg gagagttgca
     2881 ggcggttatt cacgccttct acttcgctta aacgttgcgg atgaacggcg acgatttcac
     2941 gcccttcctg atacgccagc gcaataatgt cggtgatgta gtattcgccc tgagcattat
     3001 tgttggtaag cttcgccagc cagcgtttca tatctgcgcc gttagcgatc agaatgccgg
     3061 tgttgatctc ctgaatctga cgctgctcgt cggtggcatc tttgtgctca acaatgccgg
     3121 taactttgcc gttttcacgg gtgatacgtc cataaccggt cggatcatcc agtttcaccg
     3181 tcagcagacc aatgccaccc tgcggtttag catcacgcag acgctggagt gtttcgacag
     3241 agatcagcgg cacgtcgccg tagagcatta aaatgtcttc atcatcggca aagaaaggtg
     3301 cagcctgctg cattgcatga ccagtaccca gctgttctgc ctgaagcacc cagttcaggt
     3361 tgtcgtcttt cagcgcctgt tttagcagat cgccgccgtg accgtacacc aggtgaacgt
     3421 gcgctgcgcc taattcattc gcagcatcaa tgacatgctg aaccatcgct ttcccggcaa
     3481 gggtatgcag cactttcgga agatcggaat acatgcgcgt gcctttacct gcggcaagga
     3541 tcactacgct catagcatta ttcaacatac gcgtcctgac tgtaatttga gaacgaattt
     3601 aaaccgcttc accttgaaaa aactacattt ttttcatcgt gaaatggaca gaggataaat
     3661 tgttcaataa cggattatcc ccgctgacga aagcgccatt ttcgaccatc gtcacgcctt
     3721 ttgtctgcag aaaattaagg cagtaccttc atttaagcgt caggtggatg tttttgctct
     3781 tatttcgatc aatgaataaa cgggaaaaca gggggtttta tatcagcagg atctatgtga
     3841 acgctattca ggacgggtca cacgcgcaaa aaaaaagcca gcctgtttcc agactggctt
     3901 ttgtgctttt caagccggtg ttacatcgct tttttggtca actcgataac gcgcagctgc
     3961 gcgatcgctt tggccagttc cgcagacgcc tgagcgtaat ctacgtcgcc gtgagagctg
     4021 ctaatgtgct cttcagcctt acgtttcgct tccatggctc gcgcttcgtc gagatcctgg
     4081 ccgcgaattg cggtgtcggc cagaacggtc acgttgccag gctgcacttc aagaatgccg
     4141 ccagacagat agataaactc ttcgtgaccg tgctgtttca cgatgcgaat cataccaggc
     4201 ttaatggcgg tgagcagcgg tgcgtggcca gggtagatcc ccagttcacc ttcgctaccc
     4261 gttacctgga ttttctcgac cagaccagag aacatttgtt gctctgcgct gacgacgtcc
     4321 aggtggtaag tcattgccat atcaccctcc gattaaggcg ttaaagtttt ttggcttttt
     4381 ccacagcttc ttcgatggaa ccgaccatgt agaacgcctg ctccggcagg tgatcgtatt
     4441 cgccttccat gatgccttta aagccacgga tggtgtcttt cagggagacg tatttacccg
     4501 gagaaccggt gaatacttct gccacgaaga acggctggga caggaagcgc tggatcttac
     4561 gagcacgcgc taccaccagt ttgtcttctt cagacagttc atccataccc aggatggcga
     4621 tgatgtcttt cagttcctga taacgttgca ggatggactg aacgccacgc gcggtgtcgt
     4681 agtgttcctg accaaccacc agcgggtcca gctgacggct ggtggagtcc agcgggtcaa
     4741 cggccgggta gatacccaga gacgcgatct gacggctcag taccacggtt gcgtcaaggt
     4801 gcgcaaaggt ggttgccgga gacgggtcag tcaagtcatc cgcaggtacg tatactgcct
     4861 gtacggaggt gatagaaccg gttttggtgg aggtgatacg ttcctgcaga acgcccatct
     4921 cttccgccag ggtcggctga taacctaccg ctgaaggcat acggcccagc agtgcggata
     4981 cttccgtacc ggccagggtg taacgataga tgttgtcaac gaacagcaga acgtcacgac
     5041 cttcgtcacg gaatttctca gccatggtca gaccggtcag agctacgcgc agacggtttc
     5101 ccggcggctc gttcatctgg ccatacacca gggatacttt gtcgataacg ttggagtcgg
     5161 tcatttcgtg gtagaagtcg ttaccctcac gagtacgttc acctacgccc gcaaacacag
     5221 agtaaccgga gtgctcgatc gcgatgttac gaatgagctc catcatgttt acggttttac
     5281 ctacacccgc accaccgaac agaccaactt taccgccctt agcgaacgga cacatcaggt
     5341 cgataacttt gataccggtt tccagcagtt cctgagagtt tgacagctct tcgtaggaag
     5401 gtgctgcgcg gtgaatagcc caacgctctt cttcaccgat ctcgcctttc atgtcgaccg
     5461 gttcacccag tacgttcatg atacggccca gagtcgcttt acctaccggg acttcgatcg
     5521 ggtgttcgag gtcttttaca tccagaccgc gacgcagacc gtcggaggaa cccattgcga
     5581 tggtacgcac gataccgccg ccgagctgct gctgaacttc cagcaccaga cgctcattac
     5641 cattttgcac ctcaagagca tcgtacacgc gcggtacggc atcctgaggg aattcgacgt
     5701 caactacggc gccgattacc tggacaatct ttccagtagc catcttaaat cctctacgaa
     5761 ataacctgtt taaaccgcgg cggcccccga gacgatctcg gtgagttcct gagtaatgct
     5821 ggcctgacga gctttgttgt ataccaactg cagctcttta atcaggctgc cgccattgtc
     5881 ggtcgcggct ttcatcgcca ccatacgggc ggcctgctcg ctggccaggt tttcaaccac
     5941 gccctgataa acctgagatt cgacataacg acgcagcagg gtatccagca acgccttcgg
     6001 atcgggttcg tacaggtaat cccaggattt atgtttcaga tcatcatcat ctgatgccgg
     6061 taacggcagc agctggctga tggtcggaac ctgagacatg gtgttaataa atttgttgct
     6121 gacaatgtaa agcttgtcca gacggccttc gtcgtaggcc tgcaacatca cttttaccgg
     6181 accgatcagt tcggacaggg aagggttatc ccccatgccg gtgacctggg caacaacatt
     6241 gccgcccacg gagttgaaga acgacacgcc tttcgagccg atcattgcga ggtcgcattg
     6301 aacgcctttg tcggtccagg tcttcatttc cgccagcagt tttttgaaca ggttaatgtt
     6361 caaaccaccg cacaaaccac ggtcggtcga caccaccagg tagcccacgc gtttaacgtc
     6421 gcggtcttcc aggtaagggt gcttatattc cagattaccg tgtgcaaggt gaccaatcac
     6481 tttgcgcatg gtttctgcat aaggacggct ggccgccatg cgatcctgcg atttacgcat
     6541 tttggaagcg gcgaccatct ccatcgcttt agtgatcttt tgcgtgttct ggacgcttgc
     6601 gatcttacta cgtatctctt ttgcgccggc catgagcttc tcctcaatgc cttgcggcct
     6661 gccctaaggc aagccgccag acgttaccag gattgggttg ctttgaagga atcgaggatg
     6721 cctttcagct tgccttcgat ttcgtcgttg tagccaccgg tctggttgat ctcttgcatc
     6781 aacggagcgt gatcacggtc gacgtaagcc agcagagcgg cttcgaagct gccgattttc
     6841 gacagttcaa catccgccag gtaaccacgt tctgctgcga acagaaccag agactgctgc
     6901 gcaacggaca tcggcgcata ctgtttctgt ttcagcagtt cggtcacttt ctgaccgtgg
     6961 tcaagctgtt tacgcgttgc atcgtcaagg tcggatgcaa actgagagaa cgctgccagt
     7021 tcacgatact gtgccagcgc ggtacggata ccaccggaca gttttttcat gatcttggtc
     7081 tgtgctgcac caccaacacg ggatacggaa atacccgggt taaccgcagg acgaataccg
     7141 gcgttgaaca ggttggtttc caggaagatc tgaccatcgg taatggagat tacgttggtc
     7201 ggaacgaacg cagaaacgtc acccgcttga gtttcgataa tcggcagcgc ggtcagagaa
     7261 ccggttttcc ctttcacttc acctttggtg aaagcttcaa cgtattcggc gttaacacgc
     7321 gcagcacgct ccagcagacg agagtggagg tagaatacgt cgcccgggaa tgcttcacgt
     7381 cctggcggac gacggagcag cagggagatc tgacggtaag caacagcctg tttagacagg
     7441 tcatcgtaaa tgatcagcgc atcttcaccg cggtcacgga agtattcgcc cattgcgcaa
     7501 ccagcatatg gtgccaggta ttgcagtgca gcggattcag acgcggttgc taccacaacg
     7561 atggtgttag ccagtgcgcc gtgctcttcc agtttacgta ccacgttaga aatagtggac
     7621 gctttctggc cgatagcgac atagatacat ttgataccgg aatcgcgctg gttgatgatg
     7681 gcatcgattg ccagtgcggt tttaccggtc tgacggtcac cgatgatcaa ttcacgctga
     7741 ccacgaccga ttgggatcat ggagtcaacg gctttataac cggtctgtac cggctgatct
     7801 acggactgac gttcgataac gcccggagcg attgcttcta cagcagagaa gccgtcgtga
     7861 tccagcggac ctttaccgtc gattggtgca cccagagtgt taaccacacg gcccagcagg
     7921 ccacggccaa ccggaacttc caggatacgg ccagtacact taactttcat gccttcagca
     7981 aggtcagcgt acggacccat aacaaccgca cctacagagt cgcgctcgag gttcagtgcg
     8041 atagcgtaac ggttacccgg cagggagatc atttcaccct gcatacaatc ggccaggccg
     8101 tgaatgcgga taacaccgtc acttacagaa acaatagtac cttcgttgtg agcttcactc
     8161 acaacattga actgagcaat gcgctgcttg atcagttcgc tgatttcggt ggaattcagt
     8221 tgcatgctcc agtcccctta agactgcaag acgtctgcaa ggcgctcaag acgaccgcgt
     8281 acgctgccat caatgaccat atcacccgct cggatgataa cgcctgccat tacagactta
     8341 tcgattttgc aattcagctt aactttgcgt gacagacgtt tttccatcgc agcagaaatt
     8401 ttcgcgagct gttgttcact cagtgcggca gcggaaatga cgtctacctc agcggtagcc
     8461 tcactcacgg cacgcaggtg aataaactgc tccagaacat ccgggagcgc gttaagacga
     8521 ccattctcag ccataacccg aatcaggttc tgaccgtttt cgtccagttg ctcaccacaa
     8581 actgcgataa acgactcggc gagcgtttct ggcgcaagcg cgccagagag aagctctgcc
     8641 atttgttcgt ttttggttac ctcggcggca aacgccagca tgtcctgcca gcgttctaca
     8701 ctttggtgtt cgacggcaaa gtcaaaagct gctttggcgt aggggcgagc taccgtaata
     8761 aattcagaca tcagcccctc cctccttaca gttcagcgac aagtttatcc acgatgtcgc
     8821 tgttagcagc ttcatccacg gaacgttcga tgatcttctc ggcgccagca acagccagga
     8881 tagcaacttg cttacgcagc tcttcacggg cacgtttacg ctcggcttca atttctgcct
     8941 gcgcctgggc cacgatttta gtacgttcct gttctgcctc agctttcgct tcgtccagaa
     9001 tctgcgagcg gcgtttgttc gcctgctcga tgattacctg ggcttccgct ttcgcttttt
     9061 tcagctggtc ggtcgcgctg gcctttgcaa ggtcaaggtc cttatgtgct cgttctgcgg
     9121 aagcaaggcc gtcagcaatt tctttttgac gtttttcgat ggctgccatt aatggcggcc
     9181 atacgtactt catgcagaac agaacgaaca ggacaaacgc gatggcctgg ccgaggattg
     9241 ttgcgttaag attcacagca caatgcctct atttagttaa cgttctgata ttgctcttta
     9301 aataaaagca acgcttacta cgcgacagcg aacatcacgt acagacccag acctacagcg
     9361 atcatcggga tagcatccac cagacccata acgataaaga actgagtacg cagcagagga
     9421 atcagatcag gttgacgcgc tgcgccttcc aggaatttac ccccgaggat gccgataccg
     9481 atcgcagcac cgattgccgc cagacccatc atcacagcgg cagccatgta cagcagatcc
     9541 atattcaggt tttccatgac agtctccagt ttgtttcagt taaaacgtag tagtgttggt
     9601 aaattaatgt tcttcagacg ccatcgacag atagacgatc gtcagaacca tgaagatgaa
     9661 ggcttgcagc gtaatgatca ggatgtggaa aatggcccac ggcacattca ggatccactg
     9721 tgaccaccac ggcaacagac cagcaatcag aatgaaaatc agctcaccgg catacatgtt
     9781 accgaacagt cgcaaaccga gtgaaactgg tttggacagc aggcttaccc cttcaaggat
     9841 taagttgaca ggaatgaacg cccagtgatt gaacggctgc agcgtcaact ctttcgtgaa
     9901 gccgccgatg cctttcattt tgatgctgta gaacagaatc aggataaata cgcccagtgc
     9961 catagacagc gttacgttca cgtccgcaga cggaaccaca cgcagtgcag gcagacccag
    10021 tacatgttca gcaatgtacg gcagcaggtc gataggcagt aaatccatca ggttcatcag
    10081 gaatacccag acgaagatcg tcagggccag cggagcaatc agcttgcttt tgccatggta
    10141 catgtctttc acgctaccat taacaaagcc gatcaccagc tcaatcgcgg tctgaaactt
    10201 acctggcaca ccgctggtcg cctttttggc tacgctacgg aataaaacca ggaacaacag
    10261 acccagcacc accgagaaga acatggagtc aatattgatt gtccagaagg tggctggggg
    10321 gttttgtgga tccaccagcg agaatgtacg caggtccagc tgaaggttat tcaggtggtg
    10381 tcctatgtaa tcctgcggcg tcatattttc tgaagccatg atgcctttta ccctttgttg
    10441 ttaattacag ccggtgccag tatctgaacc accagcacca aaacccacgt aacgatcagc
    10501 ggcaagaata ccgcctttaa aaccgccaac gccaccacca gtaacaccaa catcgccaga
    10561 actttgaaag cttcgccaaa tgcgaatgtc caggccactc ggcctttcgc tggtgtatgc
    10621 gcctggtgac gccaggcaaa tatcataaac aaaacgttag gcagaaagac tgccaggccc
    10681 ccgcttattg cagagacgcc ccagaagggg tctttgaggc tgaacagcaa tccacttgct
    10741 atcaccacca gtaactgaac gagcagaagc ttccgagcaa cgtttcgact cacgagcgac
    10801 acagacatca cgtttttcac tcctgctccc ttcgaggtat gccgcgtgtc gtataaaact
    10861 ttctttaagg cttagagtca agcatcaaaa agcggtcaaa ttatacggtg cgcccccgtg
    10921 atttcaaaca ataagtagcc aaaaggtgaa taaatgttta aatatttttc cagtgcatac
    10981 aattgcgact tttctgctaa ccctgttcga tcatgaaaaa ctgtaaataa cgcgtaaaca
    11041 ctggtgataa agcgtgcttc agatcacata ttgcgcatgt tcgcgcacag catatttatt
    11101 tacttggcaa atgatgcctt tgcaa
//
LOCUS       DAZUCE010000168        11104 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZUCE010000168 DAZUCE010000000
VERSION     DAZUCE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.168"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..179)
                     /locus_tag="ACHY03_003698"
     CDS             complement(<1..179)
                     /locus_tag="ACHY03_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid channel protein"
                     /protein_id="HFS3906881.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNT"
     gene            complement(225..1055)
                     /locus_tag="ACHY03_003699"
     CDS             complement(225..1055)
                     /locus_tag="ACHY03_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3906882.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDNSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            complement(1059..2531)
                     /locus_tag="ACHY03_003700"
     CDS             complement(1059..2531)
                     /locus_tag="ACHY03_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3906883.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            complement(2580..3455)
                     /locus_tag="ACHY03_003701"
     CDS             complement(2580..3455)
                     /locus_tag="ACHY03_003701"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFS3906884.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            complement(3488..4405)
                     /locus_tag="ACHY03_003702"
     CDS             complement(3488..4405)
                     /locus_tag="ACHY03_003702"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFS3906885.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKKMKII"
     gene            4523..5075
                     /locus_tag="ACHY03_003703"
                     /pseudo
     CDS             4523..5075
                     /locus_tag="ACHY03_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5309..5995)
                     /locus_tag="ACHY03_003704"
     CDS             complement(5309..5995)
                     /locus_tag="ACHY03_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFS3906886.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSIASMMAQMQMGDVKALFENKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
     gene            complement(6107..7054)
                     /locus_tag="ACHY03_003705"
     CDS             complement(6107..7054)
                     /locus_tag="ACHY03_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFS3906887.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPRLYLVEECAEEQTKAAPLPVPLVSAQ"
     gene            complement(7168..8145)
                     /locus_tag="ACHY03_003706"
     CDS             complement(7168..8145)
                     /locus_tag="ACHY03_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFS3906888.1"
                     /translation="MKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKLYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSADALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYWTTLLHELVHSTGHEKRLRRVGISTFTGRNSPEYAFEELIAEMGSAFLCAHLGI
                     AGDVQHESYIDGWLKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
     gene            complement(8238..8621)
                     /locus_tag="ACHY03_003707"
     CDS             complement(8238..8621)
                     /locus_tag="ACHY03_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906889.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            complement(8737..9366)
                     /locus_tag="ACHY03_003708"
     CDS             complement(8737..9366)
                     /locus_tag="ACHY03_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906890.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TTPVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            complement(9448..9825)
                     /locus_tag="ACHY03_003709"
     CDS             complement(9448..9825)
                     /locus_tag="ACHY03_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906891.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            complement(10021..10500)
                     /locus_tag="ACHY03_003710"
     CDS             complement(10021..10500)
                     /locus_tag="ACHY03_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906892.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            complement(10583..10975)
                     /locus_tag="ACHY03_003711"
     CDS             complement(10583..10975)
                     /locus_tag="ACHY03_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906893.1"
                     /translation="MSTFILYPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
BASE COUNT         3171 a         3006 c         2238 g         2689 t
ORIGIN      
        1 caggtatttg actcaaaact tgcccaccaa ccattattcc acccctgact aacctgaatc
       61 aacgtttcat aagcgtgact ggcgcttctg tatcctccac gtagattaag agttaatgcc
      121 tgagagcaca aaggtaaacc aagaagaatc acagttaata gtggataata ttttttcata
      181 aatgcctcat cattaaatat atttatcact tgtaatctga tttctcaatg aaacttatgt
      241 tccgcaagcc attccagaag caggctttgc catttttcta tcggatatcc attttttccg
      301 gatacaccaa atccatgccc tcccttatca aaaaaatgag cttctgcatg atgtccctta
      361 tcccggatag agttaaaata tctcagagag tgaataactg gaacatcctt atcatctgtt
      421 gcatgcacaa taaagactgg tggcatatac tctgtaacac tattttcaag actggctgaa
      481 tattcacttt cacctgcatc cagaagacgt ttccggcatc cctgatgagc aatactcctg
      541 tcaagagata taactggata tatcagagcc atccagtcag gacgcacaga agtctcttcc
      601 tcataatgga gatgagtaat atatccagca agatgaccac ctgccgaaaa ccccatacat
      661 ccaacactct taaatgttct ctccgacatt ccggcccagt aagaaataaa gttcagggca
      721 ttcttcgcat ccgcaaagga ataataacca ttaacggagg gcattctgta agaaagaata
      781 aatgccgtat atccctgacc tgctatccat ctgcctacat caagtccctc tctttcataa
      841 acaatcctcc tgtatccacc tccaggtata atcagcacac tatgttgatt agatacaaca
      901 ggctcaacaa ctgtaataag gggcacaata cctgtatata ttatgccatc gtcagtattc
      961 tcaaatagcg aattgtcagt tatgtaacgc tcataattat caagagaata tatattctcc
     1021 ctgtaatgaa tcattttttt attccctgat agcatccttt attctcctgc aacattcttt
     1081 tttcttgctc cataaaaagg ttttccatcg acagtatcat attcaagcgc agcttctgga
     1141 tggatccatc tctgtacacg tgatggcata tcaaaaccaa taaggacaag aacaacaaat
     1201 gttaagataa aagcaagaga acacattgct gtaccaagac ttgtatgaga cgcaacaact
     1261 gcaccaagaa taggcgcaca tgctccacca agtgaaccta cattataaat aaaccccagt
     1321 cctgccgcac gctgctccac attaaaataa ccactgatta ttttcggcac cagagcacca
     1381 atcccctgac caaaaacctg ctgagtaaag atcagaaggc caagtaacca tacatagtcc
     1441 cggtccacaa gaaaaacggg gataataaca agctgaccga taagaagtga tataacatat
     1501 gcttttcggg ttccaagcca gtcccccata aaaccaccta tacaacttcc agctgcagag
     1561 ccaaaacctg ccagcataaa tatgagagca acagtttctg gtgaatactg catatcaacc
     1621 ttaagccatg taggaagaaa agcctgcagt ggccatccgt agaggaaaca gaacataacc
     1681 acaagcatca acataatgcc tgttggccat cttttcccca taccctgata tataaaactg
     1741 ataaaaacag ccgcacagca caatgccata actgtgatta aagcagcgtt tgcagtgacg
     1801 cctgaaaaac aaacatatag acatgcaaat gcaatacaag acagaagaat atttatgtat
     1861 ttattctttt ctctgtacag aatatccacc attgttctga cgggtttatt ctcttgttgc
     1921 cttttttgcc agtcatcaga ctccggaaga ttcttgcgaa gataaaaagc aaataatatt
     1981 ggaagcatgc cgacaaagaa aagagaacgc cagccccata gtggtactac aatgctataa
     2041 acctgtgcag ctaaacctcc cccaatagaa aatccagaaa ttaaaaaacc acttgccttg
     2101 tttctgagat gaacaggcca gctttcgata acatatgtta cacttgagcc atattcacct
     2161 gccataccaa gcccaataac aatgcgagca ataaacatca ctgcatatcc aggagccaga
     2221 ccacaggcaa gggttccgag agagaatagc actatactta aaaccattgc catcctgcgc
     2281 cccatcttgt ccgataaagc gccaatagca agccccccaa accatcggga aataaaagca
     2341 gcggaaataa ggcttgccgc ttctattgtc gtcaagccga attcatgctg aacctctgta
     2401 agaaccagag atatcagaac gaaatcaaaa ccatcaagta aataacctgt ccatgccgca
     2461 ccgaatatct tccactgtct gggtgaaaga tgcttatacc aaacctgctt tttctgaata
     2521 caggagtcca tattaaacct cacaataata gcggatttat aatacactgg agtattactt
     2581 taagtaatac aatcccggtc ccaaagagtt gcaccaataa gaccagagtc acttctgaaa
     2641 tatgccggaa acagcattac attacaaatt tcagaatata tggaaagatg tttcgacact
     2701 ttctgaatat acccatcagc aagtccaaca cttcctccga caacaacaac ctgacaatca
     2761 agcagtaatt ttaaacttac tattaattca gcaatagtac tggcagaacg ctctgtcaaa
     2821 aattcggctt ccttatatcc ctgtctcgct aattcaaaaa tatatttggt gctatatcct
     2881 gcaagttcat tttttgctgc ccccatgatg gcccgaccag atgaaaccgc ttcaacgcat
     2941 ccccgacgac cacatccaca ttctgtatcc gttactccgg acaatatatg cccaacatgt
     3001 ccggctaatc cagaaacacc tgttaataat tttctgttaa ctacaattcc ccctccaacc
     3061 cccgtagaaa cggttataaa aaccatatca caaatttctt ttggcaaaac tgtatattct
     3121 gcccatgcgg ctgcctgggc atcatttatc acacttccat ttaaaccagt tatatcttcc
     3181 attatatttt tcagaggata ctctttcagc cctccaagat tgtctggatt caaagctgtc
     3241 aatactccat tattaataat ccctgttgat gctactgcaa atcgctccgc tgttcccttc
     3301 atttcagaaa ccagtagacg taaagcccct gtcatttcgt caggacatct ttcatgaggt
     3361 gttgatatct gttttttcag caaaaatgaa ccgtcatcag atatcaacgc cgctgaaatt
     3421 ttcgttcctc ctatatcaac agccagtgtt atcattcatt ttacctatat aacaatcttg
     3481 caattattca gattattttc atttttttat ttctgtcatg ttcttcgagc agtcgctctg
     3541 caagttttga taatacatcc agattctttt cctttaccgg agaaaaagga cttctgcata
     3601 caggagtatt tataatcccc atatagtaaa gaagagtttt tatacctgcc aggacaccac
     3661 tctgaataag agtatcaata acctggttac aggccacctg catttccttc gccttaatca
     3721 catcattatt tttgactgct tcaaatattt caagataacg ccaccccatt atattatatg
     3781 tactaccgat tcccccatct gcacctgcaa ttaaccctga tgcaaatatt tcatcgtaac
     3841 cattatataa cacaagctct ggatgattcc ttttgatttg ttccatctgg aataaatcac
     3901 cggatgtctg ttttaatgca caaaccccgg gtattgtgac aagctcattt atctgatcta
     3961 aagaaaaacg cactccgcta agagccggta tattataaac taccatcgga agtccatctg
     4021 cagaatcaat aatcttcctg taatggatac aatgctcttc aaatgaaaaa ggataataaa
     4081 atggagtaac agcagatata gcatgatatc cgtatttttt tgcagctttt gcaagtacct
     4141 cgctttctgc cgtactgatt ccgcccacat gcgctataag tgttagtctt ccatctgatt
     4201 catcggctac tgtttcaaga attttttctc gctctgcaac attctgaaga aatgcttctc
     4261 cagtagagcc tccaacatac aatcccttaa tattttgttc gatattaaaa cggatcagtt
     4321 ttcgaagact ctccatatcg atttgctggg attggtcata aggagtgggt aatgctgata
     4381 tgaccccttt aaattcacat tgcattttta atcaccttca cttaactgca taatgggata
     4441 cctagtacac catcaagtac aaagattatc caacagaaaa gaagcacaat gtgaagatta
     4501 tcaaaaaaaa gccatcagaa agctgtcatg ccgctcagtt aaggatcggt tgatcgatcc
     4561 agtgtccagt tactattctc tgcgtaaccc tctgcgacta tcttgccctg gaactgatta
     4621 cccacagccc tgaagagtcc gacactgtca cgctacacaa acggcgtctt cctctggaaa
     4681 tgatggtctg gtgaattatc agtatggccc atgagcgtaa ggaaccttca tcagattgtg
     4741 aaccggctgg acatcatgct gccaggcagt agcgccgtgc tccatgcccg ccagcggcca
     4801 ggaagcggag ccatacgctg cgtgttcact caaacagcgc agctctagca taacaccacg
     4861 ccatatccgc tctggtgcga tctgaatctg ctgaccattg atggcgtgtt ctgacgaaca
     4921 caggacacgc aagaacacga taaagcttca ctcgccaaat acatagtgga aaccagacac
     4981 tttatcctca ggtaaaaatg gtctgtctga tagaactgac cagccatcta ctgacggcag
     5041 cggccttcga tacaatgaat ctggcactaa ataagactgg caatatagcc gataatgcgt
     5101 tgaatcagtg gctatatcgt atacataaaa cagacatcat tattctgtct tgttgtccaa
     5161 cacataaaaa ctaataaaaa cataccattc cctgtcgtca gagaatttat tgataaacag
     5221 gctttattga acaaatcaaa cattcaggga actggcagtt gaacaaaacg ccagtccgaa
     5281 gactggcgca gtgataaaac attctgcatc atgccacatt ctgctcctga agatgacgca
     5341 gacgatggtc ccagtttccc aggtaatgcc cgggagtcag cagtacgcgg cgtcgctcat
     5401 tacagagcga gttaccaatc accacttctc cggccacacc acacaacgac agttgtatat
     5461 acgccatgcc ggctgccagc gggtcaatat ccgttgccga cacccacatc ctgtggtatg
     5521 ccggataccc cgaacggttc aggatgtcag ccattgccag aatcatactg ccggcaccac
     5581 aggcaggttc actgagcgtg ataaagggtt tgttctcaaa caacgccttc acatctccca
     5641 tctgcatctg tgccatcatg ctggcaatac tccagggcgt gaaaaactga ctccggtatt
     5701 tatcccccag ttccagttgc atgaacacac tccccagaaa atcacagaat ccctgctcca
     5761 gtccctcaat gacatgagca agcaaatgcg caatgtggat aacatcctgc ttttcataac
     5821 cactgacgat tcgcagatac ttctgttccc gggcctcact gaactgaagc cggttttcca
     5881 gggcaatcac actacagctg acaaagtctt caaacacctt atgacgtttg tgataacagg
     5941 ctgtccggtt aaacaacgaa atgaactcct tctgatggtc agtcatggtg gccatgtttt
     6001 ctccgtaaaa acagggaaca catggcccgg acaggggaac aggtgttccc cttcgggttg
     6061 aaatgttgat aaaacgggca tcacggacat gatacccgga ggttaattac tgggctgaga
     6121 ccagaggaac cggtaaaggt gcggctttcg tctgttcttc agcgcactcc tccaccagat
     6181 acagtcgggg ctgtggaagt gtttccctgc ccggccattc ggcctgcaga tcactgtccg
     6241 tatgctctcc gccatcgtaa cagttgtaac cctgtatacc gtgtaccggc tcgctgtacc
     6301 agtgtcggac ctcgcagtca aacaccttcg agatctcacc aaccagttcg cctgaaggcg
     6361 gataccaggg cgtgtcgatc agcagcacca gactgctcat ctcatcacgc gcatggcgaa
     6421 cattatttcc tgacggccac tccacaccgt atatccggct gtaaaggctg gtcgttgtgg
     6481 aaataccttg cagcagtcca cctgaaccat tcagctctgt agccagacgg gagggtatga
     6541 ccatcagcat gtcacagggc tgcgcctgtt ccgggtaatc cgacaaccag ctccagcagg
     6601 cttctgcatc aatgtgttca ctcagcccgg ccagaccaaa ccagtcagaa tactgacggc
     6661 tcatcagcgt ggtgatgatc tccctggcat tatccggcag attaccccag cgaacagcgc
     6721 cgatacatga ctggtgataa agacgctcaa tggtctttat cgtttcacca tccagaacgg
     6781 cattttttac cagcaacccc agccactgct caaacgcctg gttagccgga gatgacaacc
     6841 ccgttccgtg acgaaccagc gccggatatg gcggatactg cgtcgtccgt gtgggtttta
     6901 ccagccctgc acaaccggcc agaaacagct gtatactctg ctgtatggca tgacggtagc
     6961 gcggggtctc acgcccttca atccactgtg taagtacatc aagacagact gatttaccgg
     7021 taatttcaaa acggtttttg caccattcag acattgttta cgctcctctt ttttatacag
     7081 atgtaaaaag agggcattgt cccgtgaggt acaatgccct cacgggataa atggcggtca
     7141 ggttataacc gccaccggta gtaaaaatta tgctgcgcac tcctgcgaag ttcgccgcgt
     7201 cagtaaatac tctgatgctt cacgtgcctg ccggcaggcg cgaaacagcg cttttttgtc
     7261 agacttcaga accttaagcc atccgtcaat ataactttcg tgctgtacgt caccggcaat
     7321 cccgagatgt gcacacagaa aagcactgcc catctcggca atcagctctt caaacgcata
     7381 ttccgggctg ttgcgaccgg taaaggtact tatccccacc cgccgcaggc gcttttcatg
     7441 accggtactg tgtaccagct catgcagcag ggtggtccag taatcagcca cctggtcaaa
     7501 ctgcgacgac agtggcatca cgatacggtc aagatcaggc cggtagaatg ccctgtcctg
     7561 tggaagtgac gagacgctga cgccgctgcc attcagtacc tgcagcaccc gctcactgaa
     7621 ggtatctgcc ggcagtgcat ccgcgctgcc ttccgccttc accatcagat gttcaggtaa
     7681 accgtcacac tgccccacat taaacagagg attcgatttc agtaccggta cgatttccat
     7741 gagaggctcc ccctcactgt ccgtcagccg gttaccgtgt tcatccacag cctgtttttt
     7801 ccagtctttg taaatcaccg ccagcgtggc cacctcccct ttacggacgt ggccacccag
     7861 ttgttgcgcc tgacggtacg tcagccagtc attgctgaca tacccggctt catccgcggc
     7921 cagccacagc aacaggacgt tcacccctgt ataaagcttc ccggtcaggg catttgccgg
     7981 catcatctcc ggaagcccca cgccattacg cgcagagcgc cagggacgtt tccagggtgg
     8041 cacaccattt tcaatggccg taacaaccct gtcggtcaca agctgataca ggtcacgttc
     8101 cggagtttta gtcgcggtac gtgaagtatg ttttttcgtt ttcataagag catgccccct
     8161 gaaaaagatg cacgctccct gtacggtgag agtgcaccgt acagttaaat taataacgaa
     8221 aatcagactg gccagaatta ccacaacgcc agcatcacca gtgaacgacg ctcaaaatca
     8281 aacgtcttca gcgtattgct gacccacgct tttacgtcat ccacgtcata ccagtaatca
     8341 ccggcataat cttccccccg taaacacgag cctgtgacat aaggaaacag cctgtggcag
     8401 ttccccggtg tcagcatact gaggtcgcgc aataatgcag ccagtgaact gcgggttaca
     8461 gggaaagcat gctgctcatc tgaagcaccg gtatgttcac gaaaccatgt cagaagcggt
     8521 ttcaccttac ggaaagcccc cacctcctga tgttcctgtg ttcccacaac ggcaccactg
     8581 tgatggttat cacgagccag aaagtaaata tcgagtccca taacaattct cctgaataaa
     8641 gccgggactc tccccacagg gatatgtccc tgccgggtga tataatgacg gaatacgtga
     8701 tgacgttatt ccggataaag aaactacggc gttctgttac cgcccaccgg caagagcccg
     8761 cagccagtaa agctgatatt cggcctcttc cagtgctgcc cagtcatccg gggataactg
     8821 tcgcccctga ccggacaaaa actgcagcag taagctgtca aacgcatctc cccttaccgg
     8881 ggtggttaat acagactccg gagcagaaaa attctcatgc cccaggtacc gttttatcca
     8941 tttcagcggc agcgaccagc tggcaccacc gacagtggaa tgaatacgaa aacagtccgg
     9001 acacagattg tgcaacagca caaagactgt acgggcaaag aaatctcccg gctgacgtcc
     9061 gaaattcagc tcattaagag ccagaatatt ccggctgtgc tcccgctcaa tagtgacatt
     9121 caccgaccac agcccgtcgg cagaccggca gtaaaacgcc agcgtgtccg ttaccggaaa
     9181 caactctgac gcccgggtca gggagccgaa atacgcattc tgcggccgta acgcctccgg
     9241 caactgccag aaggtttcag tgacagccag aacaaaagca tgccactgct tttccggtat
     9301 tgtggaaacc tgctcaaggg tatataccac cggctcacgg gccggacgtg aagataactc
     9361 agacatggaa cattcctcat ggttaaaacg aaacaacggg gaatgtctcc cggacggaga
     9421 actcccccgt cagggtcagg tcatgaatta catcatgtcg tgccgggcaa aatattcatc
     9481 cgctccggcc accatctttc cggtactgat actgtcagcc cagatttcat gattaatttc
     9541 acgccccaga gccatcagac tgtcgagtgc ctgacgggcc tgaattcccg gtgcggaaat
     9601 ctcctcctgc tccaggtcat acaacagcca gcccagcgcc tcgccggtat cccacagata
     9661 cacgtatacc agtgaaggct ccgtctcatc atccggctcc ggggtaatac ccagccgttg
     9721 ccaggcacgt aaccgcatca gctcgccttc catctgactg atatgagcca gcagagcaga
     9781 ctgcgtctga agcgctgaat aatgagcaat taaaggtgaa tacatgatga cctccgtctc
     9841 ttaaaggaag tcctgtccct gacggaacaa aactcccgtc agggaaaaca ccggagcaaa
     9901 aacacggtgc agtaagtaaa agaaaaaccg ttacgggagc aaaacagcca tcattgcaaa
     9961 atggcagttg catcgctcac gatttgacat ccttcctgtg actgactggc atcctgcccg
    10021 tcagtctgcg tcggaaaatt caatacaggc ctgcttcagg agcccggcta tacgttgatt
    10081 cagacattcc gtccagccat cgttccatcc acgctcccat ggattgcaga catcctccca
    10141 tggattgcag acatcgttac agccattctt ccatgtcttc catgcctcca tctccatgac
    10201 agcccccagc ctgtggcctt tcacacgtat atgccacaga aacggccgga aaagtcctga
    10261 tttcagttgg tcaccaccgc ttttgtagcc cagatgacgt gcaatacttt cagtatattc
    10321 aaatgcatca ttctgcattc cagccgcctt agccagcgag ctgtacgcat cacgaagtat
    10381 cgtcatacct tccagttgat actcacacca cgcacggaca gccgacactg gtgcgattac
    10441 ccgaagaatg gtgcaggcac gccatacaac aacgtaaagg agtattaaca gactatgcat
    10501 gtgttcacct cagatacaaa aaataacggg aacacacctg ccctgcggga agcgtgttcc
    10561 cgacagggta ataaatgata aatcagcagg gctgtgcgac gattccatca ccctgcagac
    10621 caaaccggta acgcagggtg gtcagttgct ccgccatacg gcaggcagca tgccatgtcc
    10681 tgcgacggga tatccgtccg gtaaaccggt tcagataaat gacctcacgc ggtaacggct
    10741 gataccatcc tgtgtactcg ttgcgataca ttccctgccg gtaatacagg cagggtgtcc
    10801 agacaccgcc acgacggaac atacagaccc gcacgctcag attgccggcc ggattagtgt
    10861 accagcgact gtaatatgac aggttattgc gggaaaaaaa cacccgcaac tgagccgtca
    10921 ttcgccggaa aaaagaagag atgcccggtt cagggtacaa aataaacgtt gacataaaga
    10981 ctcctgtaaa aaaacgggag ccttcgcccc gacggacaaa aactcccgtc agggttaaaa
    11041 caccaccagt acagcatggt gttgctgtca gaaaaagccc gttaataaag ggcgcagtta
    11101 taaa
//
LOCUS       DAZUCE010000169        11020 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZUCE010000169 DAZUCE010000000
VERSION     DAZUCE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.169"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..535)
                     /gene="fliZ"
                     /locus_tag="ACHY03_003712"
     CDS             complement(<1..535)
                     /gene="fliZ"
                     /locus_tag="ACHY03_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFS3906894.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLP"
     gene            complement(581..1300)
                     /gene="fliA"
                     /locus_tag="ACHY03_003713"
     CDS             complement(581..1300)
                     /gene="fliA"
                     /locus_tag="ACHY03_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFS3906895.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1619..3358)
                     /locus_tag="ACHY03_003714"
     CDS             complement(1619..3358)
                     /locus_tag="ACHY03_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFS3906896.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGSGAVANTAATKSDLAAAQLLAPGTADANGTVTYTVGAGLKT
                     STAADVIASLANNAKVNATIANGFGSPTATDYTYNSATGDFTYSATIAAGTNSGDSNS
                     AQLQSFLTPKAGDTANLNVKIGSTSIDVVLASDGKITAKDGSELFIDVDGNLTQNNAG
                     TVKAATLDALTKNWHTTGTPGAVSTVITTEDETTFTLAGGTDATTSGAITVANARMSA
                     ESLQSATKSTGFTVDVGATGTSAGDIKVDSKGIVQQHTGTGFEDAYTKADGSLTTDNT
                     TNLFLQKDGTVTNGSGKAVYVSADGNFTTDAETKAATTADPLKALDEAISSIDKFRSS
                     LGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKA
                     NQVPQQVLSLLQG"
     gene            3624..5030
                     /gene="fliD"
                     /locus_tag="ACHY03_003715"
     CDS             3624..5030
                     /gene="fliD"
                     /locus_tag="ACHY03_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFS3906897.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            5055..5465
                     /gene="fliS"
                     /locus_tag="ACHY03_003716"
     CDS             5055..5465
                     /gene="fliS"
                     /locus_tag="ACHY03_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFS3906898.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEDSKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSTSLIQDPV"
     gene            5465..5830
                     /gene="fliT"
                     /locus_tag="ACHY03_003717"
     CDS             5465..5830
                     /gene="fliT"
                     /locus_tag="ACHY03_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFS3906899.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            5908..7395
                     /gene="amyA"
                     /locus_tag="ACHY03_003718"
     CDS             5908..7395
                     /gene="amyA"
                     /locus_tag="ACHY03_003718"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFS3906900.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESIVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(7429..7842)
                     /gene="yedD"
                     /locus_tag="ACHY03_003719"
     CDS             complement(7429..7842)
                     /gene="yedD"
                     /locus_tag="ACHY03_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFS3906901.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAATLDKAPLPTPLP"
     gene            8029..9234
                     /gene="yedE"
                     /locus_tag="ACHY03_003720"
     CDS             8029..9234
                     /gene="yedE"
                     /locus_tag="ACHY03_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFS3906902.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            9231..9464
                     /gene="yedF"
                     /locus_tag="ACHY03_003721"
     CDS             9231..9464
                     /gene="yedF"
                     /locus_tag="ACHY03_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFS3906903.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            9573..10241
                     /locus_tag="ACHY03_003722"
     CDS             9573..10241
                     /locus_tag="ACHY03_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFS3906904.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFLAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQDIGGKEASEIAASGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            10352..10831
                     /locus_tag="ACHY03_003723"
     CDS             10352..10831
                     /locus_tag="ACHY03_003723"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3906905.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRN
                     PTVTFWSVWEGEQLAGIGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAQD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFVDCEPFADYRFDPHSRFLSLTLCENNELP"
BASE COUNT         2731 a         2746 c         2748 g         2795 t
ORIGIN      
        1 caggcaggct ggaggatttc tgcacaagtc cggtacaggt ttggcgctga tagtgttgat
       61 acttccgtaa tgcgatgcgg taattgttgg tgctggtggt gggcagccac ggggcgagga
      121 tttcatcaag gaaaccgtcc tgcagctgat cgagggaaat attttgctcg tgtagatgat
      181 tccccaaacg gcgcagacga acgacatatt cacgcaccgt acctgggctc atttcagttt
      241 gctcaaaaag aaattgctta aagccgataa tatcgaaaaa atcactctgc gttttgcaat
      301 gtaaatcacc gcagaatcgg cacaatgccg cccatgattt ttgttccgtt tgccagccat
      361 tttcatcaag cagcgtgtcc aggcgggaaa tctcgatttt attcactatt ttgccgtcgc
      421 gaactaaggt aatgcgatcg agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt
      481 taaagtcttt aagatagcgg cttaatggcc gtcttttcag gtgctgcacc atcattaaga
      541 actcctggta gtcaaagtta aagtgcggca tttactgacg ttataactta cccagtttag
      601 tgcgtaaccg tttaatagcc tggctgtgta actgactgac ccgcgattcc ccgacctcca
      661 gcactgcacc aatctctttg agattcagct cttcctggta atagagggtt aataccagtt
      721 tttcgcgctc cggcaacgtt tcgatggctt ccatcacccg ctggcgcaga ttactgtcca
      781 gcagttgttg tagcgggttt tctcgctgat gatcatcagt aaccaattcg atgctatcgc
      841 cgtgctcttc gcgccactca tcgtaggaga agagctggct gttattggtg tcgagcaaca
      901 tttggcgata atcggcaata tctatcccta aacgttccgc tacctcagtt tccgtggcgt
      961 tgcggccaag ttcctgctcc agttgcccta ttgcctgtgc cacttcacgc gcgttgcgac
     1021 gaacgctgcg cggcacccag tcacggctgc gaagttcatc cagcatagcg ccacggatac
     1081 gctgcactgc gtaagttgta aatgccgttc cttgtagggc gtcatagcgt tcgacggcat
     1141 taagtaaccc aatgccgccc gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc
     1201 gaacctgcag gcgcaatgct tcgtgacgca ccagcgggac ataacgctgc cacagcgagt
     1261 gtttatccat tacaccttca gcggtataga gtgaattcac gataaacagc cctgcgttat
     1321 atgagttatc ggcatgatta tccgtttctg cagggttttt aatcggacga ttagtgggtg
     1381 aaatgagggg ttatttgggg gttaccggta aattgcgggc agaaaaaacc ccgccggtgg
     1441 cggggaagca cgttgctgac aaattaccat tcatgttgcc ggatgcggcg taacgcctca
     1501 tccggcctac aaaaatctgc aaattcaata aattgcaatt caccttgtag gcctgataag
     1561 cacagcgcac caggcaattt ggcgttgccg tcagtctcag ttaatcaggt tacgacgatt
     1621 aaccctgcag cagagacaga acctgctgcg gtacctggtt agcttttgcc agcacggagt
     1681 taccggcctg ctggatgatc tgcgctttcg acatattgga cacttcggtc gcatagtcag
     1741 cgtcctgaat acgggactgc gcttcagaca ggttagtagt ggtgttgttc aggttggtga
     1801 ctgcggaatc cagacggttt tgcaccgcac cgagggaaga acggaatttg tcgatggagc
     1861 tgatcgcttc gtccagagct ttcagtggat cggcggtggt tgcagcttta gtttcagcgt
     1921 cagtagtaaa attaccatcc gctgaaacat agactgcttt acctgaacca ttggtcacag
     1981 ttccgtcttt ttgcaaaaac agattggttg tattatcggt agtcagtgaa ccatcagctt
     2041 tggtgtaagc gtcttcaaaa cctgtacctg tgtgttgttg tactatacct ttactatcaa
     2101 ctttaatatc gcctgcgctg gtaccagtag ctccaacatc aactgtgaat cctgtggact
     2161 tagttgccga ttgaagagac tcagcactca ttcttgcatt tgctacagtg attgcaccag
     2221 aagtagtagc atcagtaccg ccagccagag tgaaggttgt ttcatcttca gttgtaatta
     2281 ccgtagatac ggcacccggt gtgcctgttg tatgccagtt tttagtcagt gcatcaagag
     2341 tggctgcttt gacagtccca gcattgtttt gagtgaggtt accatctacg tcaataaata
     2401 gttctgaacc atctttcgcg gtaattttac cgtcgctagc caatacaacg tcaattgacg
     2461 tagaaccaat tttaacgttt aagttagcag tatcgcccgc ttttggtgtc aggaaggatt
     2521 gtaactgagc actgttacta tcaccagaat ttgtaccagc tgcaatagtt gcactatatg
     2581 taaaatcgcc tgtagcgctg ttgtatgtat aatctgtagc tgttggcgat ccaaaaccat
     2641 ttgcaattgt ggcattaact tttgcgttat tagccaaact cgcaattaca tctgcagctg
     2701 tagatgtttt caggcctgcg ccaacagtat aggtaactgt accattagca tcagcagtac
     2761 ctggagccaa gagttgagct gctgccaaat cagatttagt cgctgcagta ttagccacag
     2821 ccccgctacc attcacatta aacccactca gccccagcgt atcagagtca attttcttca
     2881 gatcaatcgt gatagtctgg ccgtcattcg caccaacctg aattttcatt gaaccgtctt
     2941 tcgccagtac gttcacgccg ttgaactggg tctggccaga tacgcggtca atttcgtcca
     3001 gacgggattt gatttcgtcc tgaatggagt ccagatccga atcggagtta gtcccggtag
     3061 aagcctgaac cgtcagttca cggatacgct gtaagttgtt gttgatttcg gacagcgcgc
     3121 cttcagtggt ctgtgcaaca gaaataccat cgttggcgtt acgtgcagcc tgagtcaggc
     3181 ctttaatgtt agaagtaaaa cggttagcaa tcgcctgacc tgcggcgtca tccttcgcgc
     3241 tgttaatacg caagccagaa gacagacgct cgatagaact cgacagcgca gactggttct
     3301 tgttgatatt attttgagtg atcagcgaga ggctgttggt attaatgact tgtgccatga
     3361 ttcgttatcc tatattgcaa gtcgttgatt acgtattggg tttccacccg tcggctcaat
     3421 cgccgtcaac cctgttatcg tctgtcgtaa aacaaccttt agaatttttt tcacaaacag
     3481 ccattttttg ttagtcgccg aaatactctt ttctctgccc cttattcccg ctattaaaaa
     3541 aaacaattaa acgtaaactt tgcgcaattc aggccgataa ccccggtatt cgttttacgt
     3601 gtcgaaagat aaaaggaaat cgcatggcaa gtatttcatc gctgggagtc gggtcaggtc
     3661 tggatttaag ttcaatcctt gatagcctca ccgccgcgca aaaagcgacg ctaaccccta
     3721 tttcaaatca gcaatcgtcg tttaccgcta aacttagcgc ctacggtacg ctgaaaagcg
     3781 cgttgacgac tttccagacc gccaatactg cattgtctaa agccgatctt ttttccgcca
     3841 ccagcaccac cagcagcacc accgcgttca gtgccaccac cgcgggtaac gccatcgccg
     3901 ggaaatacac catcagcgtc acccatctgg cgcaggcgca aaccctgacc acgcgcacca
     3961 ccagagacga tacgaaaacg gcgatcgcca ccagcgacag taaactcacc attcaacaag
     4021 gcggcgacaa agatccgatt actattgata tcagcgcggc taactcgtcg ttaagcggga
     4081 tccgtgatgc catcaacaac gcaaaagcgg gcgtaagtgc gagcatcatt aacgtgggta
     4141 acggtgaata tcgtctgtca gtcacatcaa atgacaccgg ccttgacaat gcgatgacac
     4201 tctcggtcag cggtgatgat gcgctacaaa gttttatggg ctatgacgcc agtgccagca
     4261 gcaacggtat ggaagtttcg gtcgccgccc agaatgcgca gctgacggtc aacaacgtcg
     4321 ccattgagaa cagcagcaac accatcagcg acgcgctgga aaacatcacc ctgaacctga
     4381 acgatgtcac cacgggcaac cagacgctaa ccatcactca ggacacctcc aaagcgcaaa
     4441 cggcgattaa agattgggtg aatgcctata actcgctaat agataccttc agcagcctga
     4501 ccaaatacac cgccgtagat gcgggagctg atagccagag ttctagcaat ggcgcactgc
     4561 tcggcgactc cacgctgcgg acgattcaga cgcagttgaa atcgatgctg agtaataccg
     4621 tcagttcttc caactataaa acgctggcgc agattggtat cacgaccgat cccagcgatg
     4681 gcaaactgga actggatgcc gacaaactca ccgctgcact gaaaaaagat gccagcggcg
     4741 taggtgcatt gattgttggc gatggtaaaa aaaccggcat cacgaccacc atcggcagca
     4801 acctgaccag ttggctttcg acaacgggca ttattaaagc cgctaccgat ggcgttagta
     4861 agaccctgaa taaattaact aaagactaca acgccgccag cgatcgcatt gatgcgcagg
     4921 tcgcgcgcta caaagaacaa tttacccaac tggacgtttt aatgacctcg ttaaacagca
     4981 caagcagcta cttaacgcag cagttcgaaa acaacagtaa ttccaagtaa gcaatattca
     5041 tcgggagaca ggtcatgtac gcggcaaaag gcacccaggc ctatgcacaa attggcgtcg
     5101 aaagcgccgt aatgagcgcc agccagcagc agctggtcac catgctattt gatggagtgc
     5161 tgagcgcact ggttagagcg cgcctgttta tgcaggacaa caatcagcaa ggcaaaggcg
     5221 tctctttgtc aaaagcgatc aacatcattg agaacggact gcgggtgagt cttgatgaag
     5281 atagcaaaga cgaattaacc caaaacttga ttgctcttta tagctatatg gtcaggcgct
     5341 tgctgcaagc caatttacgc aacgatgtct ccgcagtcga agaagtggaa gcattaatgc
     5401 gtaatattgc cgatgcctgg aaagagtcgt tactctccac ttctttgatt caggacccag
     5461 tctgatgaac catgcaccgc atttatattt cgcctggcaa caactcgtcg aaaaaagcca
     5521 gctcatgtta cgcctggcaa cggaagaaca atgggacgaa ctcatcgcca gcgaaatggc
     5581 gtatgttaat gcggtgcagg agattgcaca tttgactgaa gaggttgacc cgtccaccac
     5641 gatgcaggag cagctccgcc cgatgctgcg cctgattctc gacaacgaaa gcaaggtaaa
     5701 gcagttatta cagattcgga tggatgaact ggcgaaactg gtcggtcagt catcggtgca
     5761 aaaatcggtg ttaagtgcct atggcgatca gggcggcttt gtgctggctc cgcaggataa
     5821 cctcttttga atctgaatga gtcgatggct cgcgaataat ccgattacga ctacgcttct
     5881 aatgttcccc ttgaatggag tcgaagaatg cgtaatccca cgctgttaca atgttttcac
     5941 tggtattacc cggaaggcgg taagctctgg cctgaattgg ccgagcgcgc cgacggtttt
     6001 aatgatattg gtatcaatat ggtctggttg ccgcccgcct ataaaggcgc atcgggcggg
     6061 tattcggtcg gctacgactc ctatgattta tttgatttag gtgagtttga tcagaaaggc
     6121 agcatcccta ctaaatatgg cgataaagta caactgctgg ccgccattga cgccctgaaa
     6181 cgtaatgaca ttgcggtgct gttggacgtg gtagtcaacc ataaaatggg cgcggatgaa
     6241 aaagaagcta ttcgcgtgca gcgtgtaaat gctgacgacc gtacgcaaat tgatgaagaa
     6301 atcattgagt gcgaaggctg gacgcgttac accttccccg cccgtgccgg gcaatactca
     6361 cagtttatct gggatttcaa atgttttagc ggtatcgacc atatcgaaaa ccctgatgaa
     6421 gatggcatat ttaaaattgt taacgactac accggcgaag gctggaacga tcaggttgat
     6481 gatgaattag gtaatttcga ttatctgatg ggcgagaata tcgattttcg caatcatgcc
     6541 gtgacggaag agattaaata ctgggcgcgc tgggtgatgg aacaaacgca atgcgacggt
     6601 tttcgtcttg atgcggtcaa acatattccg gcgtggtttt ataaagagtg gatcgaacac
     6661 gtacaggaag ttgcgccaaa gccgctgttt attgtggcgg agtactggtc gcatgaagtt
     6721 gataagctgc aaacgtatat tgatcaggtg gaaggcaaaa ccatgctgtt cgatgcgccg
     6781 ctgcagatga aattccatga agcatcgcgc atgggacgcg actacgacat gacgcagatt
     6841 ttcactggga cgctggtgga agccgatcct tttcacgccg taaccctcgt tgccaaccat
     6901 gacacccaac cgttgcaagc cctcgaagcg ccagtcgaac cgtggtttaa accgctggcg
     6961 tatgctctaa ttttgttgcg ggaaaatggc gttccttcgg tattctatcc ggacctctac
     7021 ggtgcgcatt acgaagatgt cggtggtgac gggcaaacct atccgataga tatgccaata
     7081 atcgaacagc ttgatgagtt aattctcgcc cgtcagcgtt tcgcccacgg tgtacagacg
     7141 ttatttttcg accatccgaa ctgcattgcc tttagccgca gtggcactga cgaatatccc
     7201 ggctgcgtgg tggtcatgtc gaacggggat gatggcgaaa aaaccattca tctgggagag
     7261 aattacggca ataaaacctg gcgtgatttt ctggggaacc ggcaagagag catagtgacc
     7321 gacgaaaacg gcgaagcaac cttcttttgc aacggcggca gcgtcagcgt gtgggttatc
     7381 gaagaggtga tttaaattca tccccggcgg caagccgggg agatttcatt acggcaatgg
     7441 cgtcggtaac ggcgctttat ccagcgtagc ggcacactct gccgtcgggc gatccacacg
     7501 ctccatcgtc ataccatcgt actcaatggt gttaccgtca cgctcaacct cgtacagctc
     7561 acgttttacg gtaacgttag tgagatcatc agacatcaat gtcaacttgc ccggcacagc
     7621 aatcacccgc tgccactgac ggcaatcaag agtatcgccc tctttagtga caatcaggct
     7681 gcctatcgct tccggactga ccagcgcacg ttgcggccct ttggtctgcc agtagcccgc
     7741 cagccagtcc ggcgcggggg ttttcacgac attgttatag ttttcgactt cggcacagcc
     7801 cgccagcaac atgagcgcgc ctgcaattgc gagttttttc atcattcgtc ctgcatgaga
     7861 agaaaagagg attgtggcat taaagccctg atgtcgccag cctttccagg ttcagagaga
     7921 aatttgatct aactattatt tttgtgaatt attaccccga aaataaattt gcatggtgta
     7981 tgattcgcga ctcttgtttc aaaccttctg agttcagagg ctacattcat gtcatggcag
     8041 caattcaaac acgcctggtt gattaaattc tgggcgccca tccccgcggt catcgcggcg
     8101 ggtattctct ccacttacta ttttggcatt actggcacct tttgggctgt cacgggtgaa
     8161 tttacccgtt ggggcggcca gctcctgcaa ctgttcggcg tccatgctga agagtggggt
     8221 tactttaaaa ttatccatct ggaaggatcg ccattaaccc gcatcgacgg gatgatgatc
     8281 ctcggtatgt ttggcggctg ctttgccgca gcgctgtggg ccaacaatgt caaactgcgc
     8341 atgccgcgca gccgtatccg cattatgcag gccatcattg gcggcattat cgccggtttt
     8401 ggcgcgcgtc tggcaatggg ctgtaacctg gcggcgttct ttaccggtat tcctcagttc
     8461 tcgctgcatg cctggttctt tgccatcgcc actgccattg gttcatggtt tggcgcgcgc
     8521 tttaccctgc tgcccatctt ccgtattccc gtgaaaatgc agaaagtttc tgccgcctca
     8581 ccgctgacgc aaaaacctga tcaggcgcgg cgtcgttttc gtctcgggat gctggtcttt
     8641 ttcggcatgc tgggctgggc gctgctcact gcgatgaacc aacccaaact ggggctggca
     8701 atgctgtttg gcgtcggctt tggtttactg attgaacgtg cgcaaatctg ctttacttcg
     8761 gcgttccgcg atatgtggat caccggacgt acccatatgg cgaaagcaat cattatcggt
     8821 atggcggtaa gtgccatcgg gatcttcagt tacgtacagt taggcgttga acccaaaatc
     8881 atgtgggcgg gaccaaacgc ggtaattggt ggtttactgt ttggttttgg catcgtgctg
     8941 gcaggcggct gcgaaaccgg ctggatgtac cgcgcggtag aaggccaggt gcactactgg
     9001 tgggtcggtc tgggcaacgt gatcggctca acgattctgg cgtactactg ggatgatttc
     9061 gctccggcgc tggccaccga ctgggacaaa atcaacctgc tgaaaacctt tggcccgatg
     9121 ggtggcctgc tggtgacata tttgctgttg tttgctgcgc tgatgttgat tatcggctgg
     9181 gaaaaacgct tcttccgccg tgcggcaccg cagactgcta aggagatcgc atgaaaaata
     9241 tcgttcctga ttaccgtctt gatatggtgg gtgaaccctg cccttatccg gcagtcgcaa
     9301 ctcttgaggc gatgccgcag ttaaaaaaag gggaaatcct ggaagtggtg agcgactgtc
     9361 cgcagtcgat caataatata ccactggatg cgcgtaatca cggctatacg gtgctggata
     9421 ttcagcaaga cgggccgacc attcgttatt taattcagaa gtaatccctc attcccgctg
     9481 tggcccacag cgggaacatc gttacctttg tatcacatag attttacccg ccccctctcc
     9541 tattcttttc agtatgatct caggaggttt ctatgtgtgg acgctttgcc caatcccaaa
     9601 cgcgtgaaga ttaccttgcg cttctcgcgg aagatattga acgcgatatt ccctatgatc
     9661 ccgaacccat tggcagatac aacgtcgcac cgggaaccaa agtccttctg ctcagtgaac
     9721 gtgacgaaca ccttcatctg gatccggttt tctggggata tgctcccgga tggtgggata
     9781 aaccgccact gattaacgcc cgcgtagaaa ctgccgcaac gagtcgcatg tttaaaccgc
     9841 tctggcaaca tggtcgggca atctgttttg ccgatggctg gtttgagtgg aaaaaagaag
     9901 gcgacaaaaa gcagccttat tttatctatc gtgctgatgg acaacctgtt tttctggccg
     9961 caataggcag cacaccgttc gagcgcggtg acgaagccga aggatttttg attgtcactg
    10021 cagcagcaga ccagggtctg gtagatattc atgaccgccg cccgctggtt ctgtcgccag
    10081 aagctgcacg agaatggatg cggcaagata ttggcggtaa agaagcctca gaaatcgcgg
    10141 ccagtggttg tgtgccagca aaccagttca cctggcaccc ggtatcgcgc gcggtgggta
    10201 atgttaaaaa ccagggtgcg gagttaattc aacctgtttg ataagcctgg agattattga
    10261 tcaacaatac tgcgtcataa gtaatctcta ttagacaaag atttcattac ctgttggcat
    10321 attgcaaaaa taacaccaat acggaatcgt catgttcacg attaaaacag atgatctcac
    10381 ccatccagca gtgcaagcat tagtggctta ccatatttcc ggcatgctgc agcagtctcc
    10441 ccccgaaagc agtcatgctt tagatgtgca aaaattacgt aacccgacag tgacattctg
    10501 gtcagtatgg gaaggcgaac aactcgcagg aattggtgcg ctgaagttgc tggatgataa
    10561 acatggcgag ctaaaatcaa tgcgtaccgc gccaaattat ttacgtcgtg gtgtcgccag
    10621 tctgatttta cgccacattt tgcaggtcgc ccaggacaga tgccttcatc gcctgagctt
    10681 agaaacgggt acacaggctg gatttacggc ctgccatcaa ctttatttga aacatggatt
    10741 cgttgattgc gaaccgtttg ctgattatcg atttgatcca cacagtcgat ttttatcatt
    10801 gacgctatgc gaaaataatg aattgccatg agccagacgc agcacattct tgcattcgac
    10861 gtgctgcgcc tttatttatc accagcagga aacgccttgt ccatagacgc cccttccaca
    10921 tgcgtcacaa gaaacctcta ttccagtgac acaatttcgc ctaattaatt acataaatta
    10981 tttaattctg accatctatt actatccttt taaccatatc
//
LOCUS       DAZUCE010000170        10994 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZUCE010000170 DAZUCE010000000
VERSION     DAZUCE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.170"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(367..1128)
                     /locus_tag="ACHY03_003724"
     CDS             complement(367..1128)
                     /locus_tag="ACHY03_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFS3906906.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSTPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLCEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCIFY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(1121..3217)
                     /gene="traD"
                     /locus_tag="ACHY03_003725"
     CDS             complement(1121..3217)
                     /gene="traD"
                     /locus_tag="ACHY03_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFS3906907.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTEHSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
     gene            complement(3214..4002)
                     /locus_tag="ACHY03_003726"
     CDS             complement(3214..4002)
                     /locus_tag="ACHY03_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906908.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGKRMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQAFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEALLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEALSASPEKALPRSWWRRLLQRGTRS"
     gene            complement(3999..4481)
                     /locus_tag="ACHY03_003727"
     CDS             complement(3999..4481)
                     /locus_tag="ACHY03_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906909.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRGGPAVMPWPDGIGISKVQERRAE
                     "
     gene            complement(4478..5071)
                     /locus_tag="ACHY03_003728"
     CDS             complement(4478..5071)
                     /locus_tag="ACHY03_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFS3906910.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGRS"
     gene            complement(5068..5586)
                     /locus_tag="ACHY03_003729"
     CDS             complement(5068..5586)
                     /locus_tag="ACHY03_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFS3906911.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            complement(5583..6263)
                     /locus_tag="ACHY03_003730"
     CDS             complement(5583..6263)
                     /locus_tag="ACHY03_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFS3906912.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTPSPRYIW
                     TEPQEEQK"
     gene            complement(6242..6961)
                     /locus_tag="ACHY03_003731"
     CDS             complement(6242..6961)
                     /locus_tag="ACHY03_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFS3906913.1"
                     /translation="MKRKILYLLALCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPSILPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            complement(6965..7714)
                     /locus_tag="ACHY03_003732"
     CDS             complement(6965..7714)
                     /locus_tag="ACHY03_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906914.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESAREKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
     gene            complement(7711..8310)
                     /locus_tag="ACHY03_003733"
     CDS             complement(7711..8310)
                     /locus_tag="ACHY03_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFS3906915.1"
                     /translation="MKKRFILSGLTVCLLMAGCQSTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            complement(8496..9488)
                     /locus_tag="ACHY03_003734"
     CDS             complement(8496..9488)
                     /locus_tag="ACHY03_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFS3906916.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRQEGIEKGRLEGIEK
                     GRQEGIEKGRQEEREKALQENYASARRMQQMGISSEKIKQILQFSDDDLRNALR"
     gene            complement(9537..10097)
                     /locus_tag="ACHY03_003735"
     CDS             complement(9537..10097)
                     /locus_tag="ACHY03_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFS3906917.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKEHTEWHRVVIYGKLAEIAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYST
                     EVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGM
                     PPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            complement(10113..10316)
                     /locus_tag="ACHY03_003736"
     CDS             complement(10113..10316)
                     /locus_tag="ACHY03_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906918.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(10407..10874)
                     /locus_tag="ACHY03_003737"
     CDS             complement(10407..10874)
                     /locus_tag="ACHY03_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFS3906919.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
BASE COUNT         2532 a         3368 c         2621 g         2473 t
ORIGIN      
        1 tgatttattc ctgtttatta aaacacggca tcactactgt ttcccgttaa tgacacctca
       61 ttaacaattt aaagaggaaa cacatcagca tggaatttct ccgtactgcc cttccgttct
      121 gggccctgtt ccttctgacc tgggtttgtg ttgtgtattt tttcatttat gaagaacctc
      181 gttgcccccc gtggatctat gacgtcatca gggccatcac tctgttcatt attttcgtca
      241 acattgttgt cattccggcg ctgtcgttac tctgacactg acgcgcactg accgtaaggg
      301 cgtggcttgt ctgccgacac cctgccacaa aacaagtttt gcatcagggt gtccggcctc
      361 tgtggattat aaatatttct tgaaggatgc catggtcacg gtgatggcca cccccagcag
      421 catggccgcc ggccatatca gcaggttcgg atacaccgac gtcggccagg acaggtaaaa
      481 aatgcaggga atatatatcg ccggtttaat agactttttt gcatggtgat aaacaaagct
      541 ggactcatac gcagcaccat accggcgcag atcacgacgc cccagtccct ccacaaccgc
      601 gatcagtatc accatcacaa acagcggaac agacaacacc aggatcgtca cccgtatcag
      661 tgtgaccacc gtgacatata ctgtggccat caggtactca cacagataat ggctcagcca
      721 gcgccccaca tcctcactgt actgtgacac actgttaccc accacggcac cggcattttg
      781 ctgcttcagc cacgacagaa aacctgtttc cacaaacagc cactgatacg ccgttgctat
      841 ccattctgtc accgttaccg atggtgtcga cagcagcaga ctgcgggtga attccgaaga
      901 cagccacccc agttctgtct tcatcactgc ctcactgtgt gctgccccct gctccggcca
      961 gaaaaaagca atcccgatat actcaaccag caggctcagc agcagggagg aaaacaggat
     1021 cccgaccagc atccacggcc agccccacaa cagattccag agcagtccgt gctgacgtgg
     1081 cggagagggt tgtgctgctc tgttcacctc acgacggggt tcagccattc tgctctcctt
     1141 ccggcatgtt cacggaggga gtctcattcc accatccctc tccggtgtgg taattacgcc
     1201 gcatggcctc tgcaattttt gcaatactct ccggcatcag cgcgtcatct gcatcagttg
     1261 caggcagggg catacggatt ttccacaact gtcccccttc cagcagagca aaggcctgtc
     1321 ctttcggcag ggtcacgata tctgccggct ccagcaaagg caccttcaca gtaccaacac
     1381 gatcctgggt actggatgtg aagtcctggc cggtcgtgac atctgcggta tcctgatgac
     1441 cggaaaccag cgttttggta taaatttcca cctgggacaa ctgggtcgtc agtaactcgg
     1501 ccgtccggtt ctccctgacc cgcagcataa tcatgttatt aaagttaccc tgcacctggg
     1561 ccgttttggc agcactaccg gtacgggcct caatatcaga agaggtctgc gtgtaagccg
     1621 tcacctgcat accggcaccg cctcccttgt tgattaacgg aatgaactca tcgcccatca
     1681 gttcattaaa ctcatcacaa tgcaggttaa ccggattttt ggtaccattc cctcccggaa
     1741 gaccggcatt cattccgtgt ttgtagatat gcccggcgac actcaccaga tccgcaaaca
     1801 tggagttacc cacggcactg gccacctcac tgtcactcag cgcatcaaga ccaatgtaca
     1861 ccaccgcctt tttacgtatc acctgctccc agtcaaacac gggacgggta tcgctcatat
     1921 ccaggtagtc aggtgccagc agttctgccg tctttcctgt ggtcagtttt tccagcaacg
     1981 gcaacaggga tgccacaatt ttgtcaaaat aggtacgatc ataacgtacc gcgcttcgca
     2041 gaccatccag tatcggatca tagagtttct ttccggcttc actgctgaga gccacctcaa
     2101 tggcccagat acgcacagca tccggctggt tctgcatggt ccggggaaca tccgcttccc
     2161 ccagtacatt catgttattc agaatggcgt tttccagttc cggcatctgt tcgctgatca
     2221 ccttaccagc ataacggata taaaggtcgg caatattatt cacataacgc ataatcagcg
     2281 tgtaatctgg gcgctccccc agtgccacca gtgcacgggc aacaatgttg acaaaccgcc
     2341 atgcaaactc acggaaggcc gcactgttac cttcaccgga taactgaccg gccacacggg
     2401 aagccacctc cgacacacga ccaaaacgcc ccacggcgtt ataacgcgca gagatttccg
     2461 gccagcccag atgaaagata taaagctcat cgcccctgcc ggcacggtgt gcttcagccc
     2521 agacacgcag caacagatcc gcatcccctt tgggatcaaa gacaatggtg acatctcccc
     2581 gacgaatatc ctgtgtgacc agcaactccg ccagccgggt tttcccgaca cgggtggtgc
     2641 catagacaat ggtgtgcccc acacgttcac ccagtgccag cgtcacatcc tgctcatccg
     2701 gctcaatacc atgcagggca ggatttcctc ccaccggcgg caggggacgg acgggattca
     2761 gcggcgagtc acgacgaaga agctgcccaa tccagggaag actgtgctcc gttttcattt
     2821 caagccagcg cgctgtccgg tacagactgc ccggctgtag atagtgtgca acctccggtc
     2881 tcagcgtgtc ctgaaggcgc tgggtgtgct tctgtgtcca gcggaagccg cgtcccagaa
     2941 acagccgctg acggctgacc ggaatttgcc gggaactcat cacataacgg ggcagccggc
     3001 gcagattgcg acggtaacgg atgatcttca ttccctgccg gaaacggacc actgccagaa
     3061 cgccaaagcc ggcggctgtg acatagctga ccgaaggcgc gagcgccacc gcccagggcg
     3121 ctgtcacaca gacaccggcc gccgttgctg ccaccacggc ggtattcagt tccacagccg
     3181 gacgcagcag agcctcaatc acataacggt tactcatgac cgggtacctc tctgtaaaag
     3241 acgccgccac catgagcgcg gcaacgcttt ctccggtgat gctgacagtg cctccagtct
     3301 gcgctgtaac accatcacct cccgccatgc gtctgccgca ttcagcagta atgccttata
     3361 caccagtggc ccgtcatgtc cgccggcatg cacatcatcc actgtcaaca gcgcctctgt
     3421 tctgaaacgg tccactttcc agcaggccag gtcttcgcgg gaaacacact gccgccggat
     3481 ctgcagacga agatacacat catgccggga caacggttct gccaggttgt gaatttcccg
     3541 acaggcagac cggaacgcct ggtctgtgtg ataaatactc tgggccacat tgtgaagctg
     3601 tgtgcgcccc tccccctgca ggtggaacag aaagcgggcc agacagctgc ggtgattacc
     3661 gtcagcactg acataccagt gaatgccatc cgtgctgaca aaacccatcc cggcatgctg
     3721 ttgctctgcc tgcgtgtaat atcccggatt cttttcaagt aacgggatat tgatgtccat
     3781 gcgtttacca cgctccagca gctccagcca gctcattccg gcatactgtg gatggtcagt
     3841 ccccacaatg cggtacacat tgatactgcc ctgtcccgtc cagtagtgtt ccgtaataaa
     3901 atccgacatt gccgcaaact ctgcggtatc ccatgcaacc agttgttcat gtgcccaggc
     3961 cggagcattc gcctgtatcc gctctgtaaa tgcccccttc attcagctct tctctcctgc
     4021 acttttgaga taccgatacc gtcaggccac ggcataacag ccgggccccc cctgccttcc
     4081 ccggatacgg ctgcagaacc acaaccacga aacagaatga ctgacgagac gccttccata
     4141 aacaaagcgc ccagcaacag aaccactgcc tgcattttca gttcacgaat atcctcagca
     4201 acagcctccc cgaaagaaat cgctgttcgc ggacattcct gctcatcgac atacatacct
     4261 gagacggata tgacggagga acatttttgt accatcacat gcccttcagg cgcatcacgc
     4321 cagcgggatg actccgtcgc ataacgctgt gtcatgcctg cctcaccacc actcagattg
     4381 ttccagacat acagcattac cagacatcct gccagcattg acggccagac aatcagaagc
     4441 cggagcagca gcataacaaa ccggtacagt gtcataatca tgatcttcct ttattactca
     4501 gtaaggtgcc attcagcagt tgcaacagac gttgtccact gatcagctca atatccggcc
     4561 acagtgtcag ccgtgcccgg ctgatgttcc ccgtacgccc cgtatggata aaaaagcccc
     4621 tgcatccctt ccggcgcagc agttcgccaa aagcatcaat atgtgccgga cgaatggttc
     4681 ttccgtaacg tttagcctgt atcagccagc gttcaccgtt aatcaccacc tgaccatcaa
     4741 gaccaccatc gccgctgtat gaggggttac ggataaccgg catcccctgt tgctcaaaag
     4801 ccagcagcaa cagttcctca aacacatacg gactgatttt gcgcagatat ttcagccgcc
     4861 gggcatcacc ctgcaattcg ttcagccgct tcattacccg tgaggccgtg gcacgataac
     4921 gctgatgccg gcgtgttctc acacacgctt tttttcgccg gctgcgccac agtgccggcc
     4981 ccaggacaac agccagcagg ctgagaacca ggcaggctgt cagtaccgga gaagcagaga
     5041 gcgcagcaga agtggcagac agcggcatca tggtgcaggt acctgtgaca ggccgttgtc
     5101 cgttatcagc accgggtaat gacttatctg taacctgcgg gccagtacag aaccggatgc
     5161 cggaaccatc accacccccg gtgccagctc acgcagtacg cgcagactgc tctcatccgc
     5221 cacattcact accattcccg ccgccttccg ctccctgagt actgctgcat tcatctgcag
     5281 ccatgtgcgg gaatgggcat catcgcccac aagaaaaagt gcaccgatgc ccggtaactg
     5341 aagccggcgc gccacaacct ctccgggtga catttcaggc gtactgaccg gtaacattgc
     5401 cgcctctccc tgcggaaaag agggtgatgg cacaactgaa gcgggaaccg gtggctgccg
     5461 gtttatcgcc tcaaaaaagg ggctggcatc cctgccgccc aggtctgcaa tcactttcag
     5521 ttctgcgcca gctgttgtac agaacagcag cagactcagt acataaaact gtctttctcg
     5581 tttcatttct gctcctcctg tggttcagtc cagatataac ggggggatgg tgtcagcatg
     5641 gcggtcgctg ccacatgcac atcagacgga ttcaggaaat tatcccggtg gagccgggca
     5701 agatgccgtc tgaccacggc ggtataacgt gcggccggcg caccacctgc cgggtggtga
     5761 taacacccgg ccacctgcag ccagctgacc ggatggcgac gccggcattc ctgcagaatg
     5821 accgctgccg tattcagact ggtgtaagga tcaaatgccg cccatgtgga cgggaaacga
     5881 tgtccattcc agcccagatt gacctgtgct atcccgacat caatacgttt tgccggataa
     5941 tgtttcagga acacctgcaa tgcctgccag gcctccagac gtgtggcata acggtaccct
     6001 tttcctgcca catttattgt ccagggccag ggtcgggcca cgtccggctg acgaaaatca
     6061 cgacgccgtt gtttcagtac tccggacatc gcttccggag acatggacgt ttccgtcagg
     6121 gaaacggcat acaaaagctc tgcgggaata ccatgggcac gggccacccg cacatacccg
     6181 tccggtacgt tctgaacggc agcacgacag acaccactgc aggccaccat cagcagaacc
     6241 gtcagaatgc cgccacgcgc catccgtcag ccccctgctg cagtaccacc ggcatttttc
     6301 cttcaccaaa acgcagccag cgtccggcat catgattcag ggtgatatga cggctgcgaa
     6361 ctttctccac aggtatgctg tgcgcatgcg cccactgacg cagaatctca tcctttccct
     6421 gactgtcgac aaggtagata tcaacctgcc gtcccgtggc cagtacggag gcaagacggg
     6481 catcacaggt gccacattcc ttcgccttca caaacagcgc cagccgtccc cccgtatctc
     6541 cggtaagtgc tcctgttttt cccatactga caggcaagat actgggatac aggcgcttcc
     6601 aggccgcatt cacctcctgc tgaaacgcca gctccttctc tgttcgttca tattcagccc
     6661 tgacccactt ttcggcccag acacgtcgct ctgccggcgt tctggcctct ataccgagta
     6721 ccgtcagcgg atcaagtccg ggagactgta tcccccgggg gccggacatc agtgactgat
     6781 aacgccggta atcctcctca ctcacccccc actgcccggc ctgctgctga agtccggatg
     6841 tggtactctg cgtcaccacc gtaccggtcg tccgttgcgc ctctgacgac atattcgtct
     6901 gcccggcgga ctgagccaga acggggcaca gtgccagcag atacagtatt tttcgcttca
     6961 tcatctactc caccctgagc accgtctgac gaccattcac ccggaacgtt gcttcaccat
     7021 ggccggcata cacaagcgtc cacccggcca cactctctcc ttcacccagc agagccacct
     7081 gagagaggct ggtatacccc cggggagcca cggctgcacg ggtactgcca ccgcgcagct
     7141 ctgtcccggt cagcacaaaa ggagcctgcc gaactgtatg aactgcgcgg gatttacggg
     7201 aggacgtcac cggagcgggc tttttctcct ccgttttttt catccccgct ttcctgctgt
     7261 ccggctgtga cggtgactcc ggtactttct gcattttctc caccagaccg ctgagtgtat
     7321 ccagtctcag ggaaagcttt tcccgggcag actcaatctg ctccagcatc tccggcagat
     7381 tctccagttg ttccttccag gatgctgtca gcacgggctg ctgctccagg actgccaggc
     7441 gcttttccat atccttcagt tgcttctccg tctctgtcag tttcattctc aggggcgcga
     7501 gttctgacgc agaaagcatt tctcttctct ggccttccag ggtcgtgatc cggctgtcaa
     7561 gaccggtcat aagcatcagt acgccatata ccactgcccc catgaccagc cccacagtga
     7621 cagacagtat caacacgccg gggaaacgac tttttcttcg ctccggcgac ataccagacg
     7681 caccctccgg tatctgcggc tgtttctcac tcattttttc agaaaccctc cggttgcctt
     7741 cacacccggt tcacgaccca caaaaggaac ggtgaccgga acaggagcca gctggctgac
     7801 cggtaactgg taaccgtcac gcagactgtg gcagaccacc ctctgcacat catccacttc
     7861 cagttgccat gccggccccg ccattaactg cagggcagta cgcagtctca gtggtcccgg
     7921 ctgatactgc accgccggca gaggctgacg gtacagcacg ccgttggccg gtccggtggc
     7981 gcagagcgtg taaccggact gtttcagggc ataacgcatg gcatcggcca ccgtgggttt
     8041 cagggaggag ggaatgcgaa tgtcgatcac ctgagaaagt ggatcacgct gtacagcctg
     8101 cggctctgtg ctgaccagca ggtaacggtc atagcgcacc acctccggtg cctgagaata
     8161 cacatccggg ctggcaggct gtacattacg gtttactgtg accgacggta ccggcatggc
     8221 cgcatcccgt tgctgtagtt tctgagtgct ctgacatccc gccatcagga ggcagactgt
     8281 cagtccggac aggataaagc gttttttcat cgggtattta cctctgcagt gataaatcag
     8341 aggtcaatgt gcagaagaag acggggctgg ctcaatgaac aactcattac ccgctggcga
     8401 aaaaaaacgc ctgcagacat gcaggcgctt tgttgacagg aaagaaagca acataacaaa
     8461 tatgtcacaa tcctgcggtt aacataaatt accgattacc ggagagcatt acgcaaatca
     8521 tcatccgaga actgcagtat ttgtttgatt ttctctgacg atatacccat ctgctgcatg
     8581 cgacgggccg atgcgtaatt ctcctgaaga gccttttctc tctcctcctg gcggcccttc
     8641 tcgatacctt cctggcggcc cttctcgata ccttccaggc ggcccttctc aataccttcc
     8701 tggcggccct tctcgatacc agccagttcc agtcgttcag cgatagtcat tagatcatcc
     8761 tcatgtcttg gcagacgttc tgccatcgcc cgtataaatt caggtgaagc tgaatcacat
     8821 tctttcacca tatagttaaa caatgtcacc acctgagtgt gtgtttcctg tatctgaatc
     8881 aactgtgaca aaaagtcaag atgaaccatc agatccactt tcttctgccg gagcttcatc
     8941 ataagcatca gcgcagccat ctgcttatag cgacggatac cctcatcatc aagaagaccg
     9001 acatcaatca ccctgggtgg cctgccttct ccataaagct cccgaccaaa cgccggatca
     9061 tccagacagt caggccagtt catgctgtat ggataaggac tgggtacacc atggtaaaaa
     9121 agcaccggta tcacaacggg agcacacgca tgtgctttca ggtggcgatg tatcaccgcc
     9181 agaacatagc gcatcattcg cagggccata tacttatccg ccttgctctg gtgctctgtc
     9241 agcgtataaa tgtatccatc cctgccatct gtggttttca tactccacag aacatcacag
     9301 gcaaactggc gaagatccgg atccacaaac gtggtcggct ccagttgcag ggtgttcata
     9361 tcacaccggt tcagttgttc cgggggcaac gcgcctttca ggaaatccct cgcaacagaa
     9421 ggacattcca tcatcgctct gaacgccgca tcatgtggcg tgggggtagt gggtgtgttt
     9481 tcattcacaa ccgcctcctt gaattaaaag tggggcggta agccccgtgg ttaacatcag
     9541 atgacatgga taatccgtcg ttcaacgcca tacccacgcc ccataaaggg gatatcatcg
     9601 tcaaagtcca tcggcggcat tcccccgctt cctgaggaag cctgaccgtt ttttcccgtt
     9661 gattgttgtg cattttgtgg tggttgtccc catcctccct gagcaggagc agatgaaaca
     9721 tttccaccac cctgctgctg acgcggagac cccagcatct gcagggtacc attgacactc
     9781 acaacaactt ccgtggaata acgatcaaca ccattctgct gatcgaacca cttccgggtt
     9841 cttaactgac cttcaatata cacctgtgaa cctttacgaa ggtattcacc ggcaatctca
     9901 gccagttttc cgtaaataac aacacggtgc cattcagtgt gttccttcat ttcaccgctc
     9961 tgtttatcac gccaggtatc agtggtggcc agcgtcagat ttgccactgc acctccgccg
    10021 ttggggaaat aacggatttc cggatcctgt cccagatgac caatcagtat gactttattc
    10081 actccacgcg atgccataac aactccttat cctcacacac attcacgctg agtccagatg
    10141 ccgttttcca gagcccggac tgcctgctca taatttctga aatactcttc ggattcccgg
    10201 gacacaggcc cttcgtcgca ctgagtacca atataataac cacgagcact gctgaggatc
    10261 tgcagaggta aggtatagcc cgctttggcg gccagaattc cgcttgtcgg tttcataaca
    10321 tgtttcctta aagttaaacg ataagaaaac agacctccct gatggttggt ctgttttcat
    10381 cagggaggtt gagttgaaaa atacgatcag aaagaatttt ctgcgtattc ccgcggggta
    10441 ttgccattct gtggcggtat ggattccgat ttttcagtct tataaaccat gtcctggcct
    10501 attttgatcc actccacctt gatcaggcgg gctttcagac tgacgcgttg ctcaccggca
    10561 tgttcacccc ggttaagggt aaaaatgtca gtagaggggt tgctcatggt aaaaccaatc
    10621 agcaccttgc gatcttcatc caccgctttc tggcagcggg caatcaggga agttgcctct
    10681 ttaccggcaa ccgtcacatc aaaacgcaca taggacgggc tgtccgtcgg accactcagt
    10741 gcattcacaa cacaactgag aaatgtcccg ttctgatgat taacctgacg aatgttgctg
    10801 agatacccca ggcctttcag tgtcagattg aagtactcag ttttagcgtt tgcagaagaa
    10861 gtatttgcag tcataatgat atctccattc tcagtatcgg aaagacagag aacatcatca
    10921 tccctgacgg gttgtgatat tcccgtcagg gagcaggaac agaactattc cggctgactc
    10981 agtaacagtt aaag
//
LOCUS       DAZUCE010000171        10981 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZUCE010000171 DAZUCE010000000
VERSION     DAZUCE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.171"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            64..396
                     /gene="hdeA"
                     /locus_tag="ACHY03_003738"
     CDS             64..396
                     /gene="hdeA"
                     /locus_tag="ACHY03_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFS3906920.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            512..838
                     /gene="hdeB"
                     /locus_tag="ACHY03_003739"
     CDS             512..838
                     /gene="hdeB"
                     /locus_tag="ACHY03_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFS3906921.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            902..1549
                     /locus_tag="ACHY03_003740"
     CDS             902..1549
                     /locus_tag="ACHY03_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFS3906922.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1601..2371)
                     /locus_tag="ACHY03_003741"
     CDS             complement(1601..2371)
                     /locus_tag="ACHY03_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFS3906923.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLHEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLEDPANHSPLIVLDH"
     gene            complement(2368..3360)
                     /gene="shuU"
                     /locus_tag="ACHY03_003742"
                     /gene_synonym="chuU"
     CDS             complement(2368..3360)
                     /gene="shuU"
                     /locus_tag="ACHY03_003742"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFS3906924.1"
                     /translation="MLKDSFSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(3411..4034)
                     /gene="chuY"
                     /locus_tag="ACHY03_003743"
     CDS             complement(3411..4034)
                     /gene="chuY"
                     /locus_tag="ACHY03_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFS3906925.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSDRAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRVQNQECHGFVRRADVAAHIHELANAPALNKQIYSLIEPDLKPA"
     gene            complement(4034..4528)
                     /gene="hutX"
                     /locus_tag="ACHY03_003744"
     CDS             complement(4034..4528)
                     /gene="hutX"
                     /locus_tag="ACHY03_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFS3906926.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPSSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLNEQVSAFHTLAASL
                     KEHA"
     gene            complement(4541..5878)
                     /gene="hutW"
                     /locus_tag="ACHY03_003745"
     CDS             complement(4541..5878)
                     /gene="hutW"
                     /locus_tag="ACHY03_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFS3906927.1"
                     /translation="MNANNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLALT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDEAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVVNAPAIVREKP"
     gene            complement(5898..6812)
                     /gene="shuT"
                     /locus_tag="ACHY03_003746"
     CDS             complement(5898..6812)
                     /gene="shuT"
                     /locus_tag="ACHY03_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HFS3906928.1"
                     /translation="MPRIITRPFLFSPLTLCISAVASAAKTAVKRKKLFTAVLALSWA
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGDALVTQISQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVAPHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
     gene            7494..9476
                     /gene="chuA"
                     /locus_tag="ACHY03_003747"
                     /gene_synonym="shuA"
     CDS             7494..9476
                     /gene="chuA"
                     /locus_tag="ACHY03_003747"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HFS3906929.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     IDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
     gene            9525..10553
                     /gene="chuS"
                     /locus_tag="ACHY03_003748"
     CDS             9525..10553
                     /gene="chuS"
                     /locus_tag="ACHY03_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HFS3906930.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MHGDIREILAALESVGETKCICRNEYAVHEQVGTFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(10614..>10981)
                     /locus_tag="ACHY03_003749"
     CDS             complement(10614..>10981)
                     /locus_tag="ACHY03_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3906931.1"
                     /translation="NFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFV
                     ALINGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMI
                     LNLKRINELVRHQHIDYLV"
BASE COUNT         2827 a         2607 c         2772 g         2775 t
ORIGIN      
        1 tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt tgaggatatt
       61 acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc agttgtgagc
      121 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
      181 gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt tgctgaagcg
      241 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
      301 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
      361 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
      421 ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc ctcctgttca
      481 tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt
      541 tatttttatg ggcgctgtag cggctttgtc actggtgaac gcacaatctg cgctggcagc
      601 caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc caaaagcaat
      661 gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg gcgataccgt
      721 tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact gcaagaaaaa
      781 cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg
      841 aggtacaagt aaaaaggagt agcaagttga gccatcttgc tgctcctttt tgcattttta
      901 tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg gcgctattgg
      961 catggaaagg caaatgcgcg gcaaaggagc agggttacgc acacatgtat taattggcat
     1021 gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga
     1081 tcacgtcgga ctggacccca gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat
     1141 cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtttgacga cggcagcgga
     1201 tatctgggtg accgccgcca taggtatggt tattggcagt ggtatgtacg aactgggtat
     1261 ttatggttca gtgatgacct tgctggtgct ggaagtcttc catcaattaa ccttccgcct
     1321 gatgaacaaa aattaccatt tacagctgac actagtgaac ggtaatacgg tatcaatgct
     1381 cgactggttt aagcagcaaa aaataaaaac tgatttagtt tcgttgcagg aaaatgaaga
     1441 tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag aagatctgtt
     1501 gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat ctgtatagtt
     1561 atttattcgc aggagtacca gtgcggtact cctgttgctt ttaatgatcg aggacgatca
     1621 gtggtgaatg gttggcagga tcttcaagta cggtaatatc cgcgccgtag agcattgtca
     1681 gcgcctgctg tgtcaatacg tcctgtggtt taccatttgc gataaccttt cctttttgca
     1741 ttaatacaac acggtctgca taacgcgcgg caagatttag atcgtgcaat atgcagcaga
     1801 cattgaactg ccgctcgtga actaactggc gtaataaacg aaacaagtgc tgctgatggt
     1861 gaatatccag cgccgaagtt ggttcatcaa gaaataacca tttcggtgag ggagtaggtt
     1921 cccagagttg caccagcagg cgcgctagtt gcacgcgctg ttgttcacca ccggataatt
     1981 gccgatagtc gcggtttgct aacgcctgac agtcacataa cgccattatt tgtgctgttt
     2041 cattatcctg attaccagta cgatgcggat gccgccccat ctggataact tcttgtacgc
     2101 tgaatggaaa cgccatgtgg ctattttgac gcatcacggc ccggtgtttc gccagttcag
     2161 taatcgacca ttcatgtaat ggcttattaa acaaccgaca ttcgccgcta tcaggttgaa
     2221 gatagccggt taactggcga agcagcgtag attttcccgc accattaggg ccgagaatgg
     2281 caacaatttc cccaccggga aaagtcagcg agacattatc agtcagacgc cgcccctgaa
     2341 ggctataaac cagattctgg gcgctgatca tgtgatcctc cgatttcgca gaatcaacca
     2401 cataaaataa gggccaccaa gcaggctggt taataatccc acgggcattt ctgctggctg
     2461 taccagcgtg cgggcaagcg tgtctgccat cagcaataaa caggctccgg cgagggcgga
     2521 acaagggatt agccagcggt gatttgctcc ggtagtcatg cgaatcagat gcgggatcac
     2581 caggccaata aagccgataa tgccgcttac cgatacggca gcaccaacga gcagtgagct
     2641 cactaacagt aattgctggc gtttacgctt aacgttcacg ccgaggtaat gggcttcttc
     2701 atcaccgagc tgcagtaaat tcagcgttcc ggcgagacaa gttgttgcga taatggccgg
     2761 aaggatgaat gaactggcaa ccaataacgt cggccattgc gcctgtccaa gattgcccat
     2821 catccacaat gtgagttgtc gtagctgctg ctcatcgccg atatagctca ggatgccgat
     2881 agctgcgccg cacagggcat tgatggcgat accggcgagt aataattgca tcatgctgcc
     2941 atcgcgatgg cgctgcgtga tgagaaaaat gatggtgcag actactaaac ttccggcaat
     3001 ggcaaacacc atttgctcat agagcaccag caccactggg aaggagaagg gcagcacaat
     3061 agcaacgcca accattaatg cagaaccgct actgacgccg agtaatccag ggtcagccat
     3121 tgggttgcga aaaagcccct gcataattac ccctgcggtt gccaacgctg cacctaccag
     3181 caccgccagc aaaacccgtg gtagacgaat attgagccag atatcgcggt attcctcatc
     3241 aaaaagggca tcaaaactga tctttaacgc tccctgactg gcaccgaaca gcaccagggc
     3301 aagcaataat agtgatagcc ccataaagac cctggcggat gaaaatgaat ccttgagcac
     3361 gcaaaactcc gttatgacgt tgcggcgcgg agagtctgcg ccgcatggga ttacgccggt
     3421 ttcaggtcag gttcaatcag actgtagatc tgcttgttga gtgctggcgc gtttgccagt
     3481 tcatgaatgt gtgcagcgac atcggcacgg cggacaaaac catgacactc ctgattttgt
     3541 acccgttgtg ctttgcctgt tgctgcgccg tcaagcagac caccagggcg gagaatggcg
     3601 tagtctagct ggctggtttg cagccagctt tccgccagtg tcttttcgcg caccgcctga
     3661 ccaaacgcgg cttttgcccg atccgataag aacggccaac tatcgccgca tcccaaagaa
     3721 gtcaccagaa tcatgcgact gatgcctgcc ttttcggcct catcaatcac tgttctgtgc
     3781 gccagataat cctgagctcc tcccattgtt gagatgataa gcgcatccgg gccagctgcg
     3841 cggcaagcag ccgcaactac gctggcatca caggcgtcgc ctgtaaaaac ctgtacgcct
     3901 tgttgcgcca gtttcgtggc ggcatcggca tgacgaatga cagcaacgac cggacgttgt
     3961 tccgccagtg ccagttcaag cgttctggcg cccacacctt ttccgcctgc gccaaagagt
     4021 agccacggcg tcatcaggcg tgttccttta acgatgccgc cagagtatgg aaggcgctga
     4081 cctgttcgtt caggagctga cgatggtcat ccctgccgag gaagattttc agcatggcgc
     4141 tgccttcttt gttgaaaaag agaatggacg cggtgtccat gcccataaat ttacgttcga
     4201 ttaaggcaat atgcgtgcag ttttctgctt tgatatgccc ggacatgccg tgctttccgc
     4261 gtaggttgaa atagccgtgg cgatggaaac ccgaaggcag ttcgccgcta aattcgagga
     4321 tgacatcggc agtatgtact aacgtggtga cgttgcccca ttcgcagacg gtatcccaga
     4381 cagtatcgaa tttatcgcct ggcactacgg tagaggaggg tagatttctc actacctcca
     4441 gcagtgtggt gttgtattgt tcggctacca cctccagcgt accgtccggc tccgttttca
     4501 aaaattcttg taacgagaca tggctcataa atcactcctg ttatggtttt tcacgcacaa
     4561 tcgctggcgc attaactacc tgaattagtt cattaagaga ctgcaaaata ttgcttgccc
     4621 agaaacgacc ttcattagtc agccgcaggc aagttgaagc atcgcgactt aagccttttt
     4681 ggtgccattg agccagtaac ggcgcgagtt tttcggcatg tggtgtaagt tcatccagcg
     4741 gtacacgcgc tgtttcaaca cctgactgca aggtatgacg ccactgcgca ttacgttcgg
     4801 cgttacgcat gatcatcatc agcggttttt tgcctgccgc gacggattca tgccaggtct
     4861 gaagattgcg ttcgttcatc caggagtaac cattaatcga accgccggct ccggaaccaa
     4921 aggccagaca atcggcacct tgttttatca gcaggttata gagattgcgt tcgcgtgtgg
     4981 tacggcccca gtggctgtta ctaatacagc gccaaccggc ttcgtccatg aagtcacacc
     5041 cttgcagata aagatcgcga cgttctgccg gagagggcac ggtagtacgc ccattttcca
     5101 cggctttgcc cagcggtgta ttgggcagga cattgagcgc atagagatcg acgccatcga
     5161 gaccgatatc gcgggcaata gccagatctt ctccccaggt ttgcgcatct tgacccggca
     5221 ggccaaacag caggtcacag accaccgcag cacggtcacg tttgaccagg ctttccataa
     5281 acgcaatagc ggttgggcca tccgaggtgc gggccatttt cttgcggatt ttgctgttaa
     5341 acgactggat accaattgag aagcggtttg ctcctgcatc aaggcaagca tcgattcgct
     5401 cagcgtcaaa attcaatacc cggccttcga tagtgatttc acaatcaggg gccagtggca
     5461 gcttttctcg taatgtggtg atgattctgg ccaaatcgtg tgccgaaagc gcggaaggcg
     5521 taccgccacc gaaatagacc gcgtggatgg gggcagactg atgcaatacg ctgtccgctt
     5581 ccatttctat ttcacgaatc agggcgtcgg tataatgagc acacgcatct tcattaaaac
     5641 ggttctgata aaaaccacaa aacgtgcagt gtgtcgcaca aaaaggaatg tgtaggtaaa
     5701 ccagtcgttt acgtggtgat accgtttgat tgatcatctc ttgccaggtt agtgccagtt
     5761 gctctttggc aactggaatg gccccacgaa acggcatcat ggcgcgcctg tctttaaatg
     5821 gctgatcacc gtctaaagca aaatgtggcg tgagatccag ggtgttattt gcgttcattc
     5881 gtctgattta aatccattta ttgcggccaa agctgctggt gtaaagactc aacgacatca
     5941 gcaatacgcg gacccattcc cagaatcagg ttttgatcaa cggtgataat gcgctggttt
     6001 ttccatgcgg cagtgtgggt aattccagca atagaacgta gccgattaat atcgccatcg
     6061 accatttgtg aggttacgac aatcacttca gggttagccg caatcagtga ttctgcactg
     6121 tagcttttgt actgctggtg gggagcgacg ttttctgctc cggcaagcga aagaatggcg
     6181 tccgcgacgc tgcctttacc ggcaacttgt ggcgcacttc cgcctgcgga gagaataaac
     6241 atggctttaa ccggggcttt tttggccgcc acgttttgct gtactcgctc caggcgttgg
     6301 ctgatttgtg tcaccagcgc gtccccttgt tcaggaacct gtagcgtttt tgccagttgg
     6361 cgaatgttgg cgtacatctg ctcaagggtg gcaggcacac gcggcagggt gacgacgttg
     6421 actttctgcg ctcgtagttg gtcgagcaca atttgtggcc cggcatcctg ccaggtaatt
     6481 acgctatccg ggcgaagtga caaaatgcct tcgctgctga gttgtttcca gtagccaata
     6541 tgcggcagtt tggcggtttc tggtggataa gatgtcgttt catcgacacc aaccacgcgt
     6601 tcgccagcgc ccatcgcgta gattagctcc gtcagcgatc ctcctgcgac cacgatacgt
     6661 tcggcagccg ttacgctaaa agcccagcta agagccagta ctgccgtaaa caattttttt
     6721 cgttttactg cggtcttcgc ggcagaggcc acggctgaaa tacataacgt cagtggggaa
     6781 aaaagaaacg gcctggtgat gatccttggc ataatatgag aactcttatc aaattgagaa
     6841 tgatcatgat tatcatttga gttatatatg aaatacaacc gtcgattcaa tacgcaaggc
     6901 gttacaagcg tatttatttt tgatttttat caagatgctt cgtcagctaa cgttcttgtt
     6961 accgactaaa tttcatcgcg ggaaaaagct gagaacccag agatatcgag gcttgcagag
     7021 caaagagaag aaaaaattat cactgttaac gacaaaattt agtgatgagg catgggggga
     7081 taaccataaa aacaaggtgg tttacagggc gcaggatagg tttcataacc ccctgtttat
     7141 tgtgttaatg gtggttaaaa aaaacttgat cataaaaatt tgatcgataa taattctcat
     7201 tatcacgtct acatttaaca aaacacgacc atatggataa caaaaccata gcatttgcaa
     7261 gggataacgc acagctctct tcactcctga tggggcatat cgttttgcct gacaaagagg
     7321 gcgtgctttt gcgtaattgc cgagttatca ggcaatttca tgggatataa acgcctgtaa
     7381 actcacccgg cgaatattat cgctctgaat tttaaataaa tttatttttc gccagccacc
     7441 ccggtattaa cccaggcagc cagcattttt tattttcctc atggagaatc gctatgtcac
     7501 gtccgcaatt tacctcgttg cgtttgagtt tgttggcctt ggctgtttct gccaccttgc
     7561 caacgtttgc ttttgctact gaaaccatga ccgttacggc aacggggaat gcccgtagtt
     7621 ccttcgaagc gcctatgatg gtcagcgtca tcgacacttc cgctcctgaa aatcaaacgg
     7681 ctacttcagc caccgatctg ctgcgtcatg ttcctggaat tactctggat ggtaccggac
     7741 gaaccaacgg tcaggatgta aatatgcgtg gctatgatca tcgcggcgtg ctggttctta
     7801 tcgatggtgt tcgccaggga acggataccg gacacctgaa tggcactttt ctcgatccgg
     7861 cgctgatcaa gcgtgttgag attgttcgtg gaccttcagc attactgtat ggcagtggcg
     7921 cgctgggtgg agtgatctcc tacgatacgg tcgatgcaaa agatttattg caggaaggac
     7981 aaagcagtgg ttttcgtgtc tttggtactg gcggcacggg ggaccatagc ctgggattag
     8041 gcgcgagcgc gtttgggcga actgaaaatc tggatggtat tgtggcctgg tccagtcgcg
     8101 atcggggtga tttacgccag agcaatggtg aaaccgcgcc gaatgacgag tccattaata
     8161 acatgctggc gaaagggacc tggcaaattg attcagccca gtctctgagc ggtttagtgc
     8221 gttactacaa caacgacgcg cgtgaaccaa aaaatccgca gaccgttgag gcttctgaaa
     8281 gcagcaaccc gatggttgat cgttcaacaa ttcaacgcga tgcgcagctt tcttataaac
     8341 tcgccccgca gggcaacgac tggttaaatg cagatgcaaa aatttattgg tcggaagtcc
     8401 gtattaatgc gcaaaacacg gggagttccg gcgagtatcg tgaacagata acaaaaggag
     8461 ccaggctgga gaaccgttcc actctctttg ccgacagttt cgcttctcac ttactgacat
     8521 atggcggtga gtattatcgt caggaacaac atccgggcgg cgcgacgacg ggcttcccgc
     8581 aagcaaaaat cgatttcagc tctggctggc tacaggatga gatcacctta cgcgatctgc
     8641 cgattaccct gcttggcgga acccgctatg acagttatcg cggtagcagc gatggctaca
     8701 aagatgttga tgccgacaaa tggtcatctc gtgcggggat gactatcaac ccgaccaact
     8761 ggctgatgtt atttggctca tatgcccagg cattccgcgc cccgacgatg ggcgaaatgt
     8821 ataacgattc taagcacttc tcgattggtc gcttctatac caactattgg gtgccaaacc
     8881 cgaacttacg tccggaaact aacgaaactc aggagtacgg ttttgggctg cgttttgatg
     8941 acctgatgtt gtccaatgat gctctggaat ttaaagccag ctactttgat accaaagcga
     9001 aggattacat ctccacgacc gtcgatttcg cggcggcgac gactatgtcg tataacgtcc
     9061 cgaacgccaa aatctggggc tgggatgtga tgacgaaata taccactgat ctgtttagcc
     9121 ttgatgtggc ctataaccgt acccgcggca aagacaccga taccggcgaa tacatctcca
     9181 gcattaaccc ggataccgtt accagcactc tgaatattcc gatcgctcac agtggcttct
     9241 ctgttgggtg ggttggtacg tttgccgatc gctcaacaca tatcagcagc agttacagca
     9301 aacaaccagg ctatggcgtg aatgatttct acgtcagtta tcaaggacaa caggcgctca
     9361 aaggtatgac cactacttta gtgttgggta acgctttcga caaagagtac tggtcgccac
     9421 aaggcatccc acaggatggt cgtaacggaa aaattttcgt gagttatcaa tggtaatcat
     9481 ctgccccgat atttcggggc atttatctgg aaggaagaga gacaatgaac cactacacac
     9541 gctggcttga gttaaaagaa caaaatcccg gaaagtacgc gcgtgacatc gcaggtttaa
     9601 tgaatatcag cgaagcagaa ctggcatttg cacgcgtcac gcacgatgcg tggcggatgc
     9661 acggcgatat ccgtgaaatt ctggcggcgc tcgaaagtgt tggcgaaacc aaatgtattt
     9721 gtcgtaatga atatgcagtc catgagcaag ttggtacgtt cacaaaccag catttgaacg
     9781 gacatgccgg attgatcctc aatccgcgcg cgctggattt acgtctgttt ctcaatcaat
     9841 gggccagtgt tttccacatc aaagaaaaca cggctcgtgg cgaacgccag agtattcagt
     9901 tctttgatca tcagggcgat gcattactaa aagtttatgc caccgacaat accgatatgg
     9961 cggcatggag tgagcttctg gcacggttta tcaccgatga gaatacgccg cttgagttaa
    10021 aagccgttga tgcgccagtt gttcaaacgc gagccgatgc cactgtggtc gagcaagagt
    10081 ggcgggcgat gaccgacgtt catcagtttt ttacgttgct caagcgccac aacctgacgc
    10141 gccaacaggc gttcaatctg gtggcagacg atttggcctg caaagtatcc aacagtgcgt
    10201 tggcgcaaat tcttgaatct gcacagcagg atggtaatga aatcatggtg tttgttggca
    10261 accgtggctg cgtacagatt ttcaccggtg tggtagaaaa agtggtgcca atgaaaggtt
    10321 ggctgaatat tttcaacccg acgtttactc ttcatctatt agaagagagc attgctgaag
    10381 cctgggttac ccgtaaaccg accagcgatg gctacgtaac cagtctggaa ttgtttgccc
    10441 atgatggtac gcagatagcg caactttatg gtcaacgtac agaaggcgaa caggagcaag
    10501 cgcaatggcg taagcaaatt gcttcgctga taccggaagg cgttgcggca taacgtataa
    10561 ttaacgggag gtctggctat ttattgctgg gcctctttat attgatcaat cgttcacacc
    10621 agataatcaa tatgctgatg gcgtaccagc tcattgatcc gcttaagatt gaggatcatc
    10681 atgatattgt gtttatggca gtagaacgtt tttagcgata ttttcagttt ctccagaata
    10741 tcttccgttg tcatttgatt cgcaatgcag ctcagaacct gatgttgctt gcgcgaaagt
    10801 gacacattcg cttcaggcac agagcagtaa ctgccattga tgagtgcgac aaaatcaatg
    10861 atagtgatag tccgcggaac aaaggtcacc ggcactttcc ccaggcagcg tttaatatta
    10921 aagggggaga aaataatgac atggacaggt tttaatcgtt caatttgagt gagaaaatta
    10981 g
//
LOCUS       DAZUCE010000172        10930 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZUCE010000172 DAZUCE010000000
VERSION     DAZUCE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.172"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1821)
                     /gene="selB"
                     /locus_tag="ACHY03_003750"
     CDS             complement(<1..1821)
                     /gene="selB"
                     /locus_tag="ACHY03_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFS3906932.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRD"
     gene            complement(1818..3209)
                     /gene="selA"
                     /locus_tag="ACHY03_003751"
     CDS             complement(1818..3209)
                     /gene="selA"
                     /locus_tag="ACHY03_003751"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFS3906933.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPTWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAVCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3307..3915)
                     /locus_tag="ACHY03_003752"
     CDS             complement(3307..3915)
                     /locus_tag="ACHY03_003752"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFS3906934.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3986..5122)
                     /locus_tag="ACHY03_003753"
     CDS             complement(3986..5122)
                     /locus_tag="ACHY03_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS3906935.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5125..5487)
                     /locus_tag="ACHY03_003754"
     CDS             complement(5125..5487)
                     /locus_tag="ACHY03_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFS3906936.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKVTDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6024..7937
                     /gene="mtlA"
                     /locus_tag="ACHY03_003755"
     CDS             6024..7937
                     /gene="mtlA"
                     /locus_tag="ACHY03_003755"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFS3906937.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEDDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8167..9315
                     /gene="mtlD"
                     /locus_tag="ACHY03_003756"
     CDS             8167..9315
                     /gene="mtlD"
                     /locus_tag="ACHY03_003756"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFS3906938.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDANAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9315..9902
                     /gene="mtlR"
                     /locus_tag="ACHY03_003757"
     CDS             9315..9902
                     /gene="mtlR"
                     /locus_tag="ACHY03_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFS3906939.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9914..10123)
                     /gene="yibT"
                     /locus_tag="ACHY03_003758"
     CDS             complement(9914..10123)
                     /gene="yibT"
                     /locus_tag="ACHY03_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFS3906940.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10408..10770
                     /locus_tag="ACHY03_003759"
     CDS             10408..10770
                     /locus_tag="ACHY03_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFS3906941.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVDAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
BASE COUNT         2624 a         2947 c         2806 g         2553 t
ORIGIN      
        1 gtcgcgcaat aaatgatcat ttccacgacg acgcgtaaag ccaatgcggt caaaatattc
       61 caggatttga atcgccagtt ttcgcccaac ccctaagcga tcgcggaaat ccgccgcgca
      121 ggttgaacca cattcctgat cgagatcgcg gatcatattg gcaaactcga caatccgatc
      181 gttacggtaa taacgatctt tgacgatcgc ggtaattatc ccctgttggg ccgcctggcg
      241 tagcgtcaga cgcattgcct gctcgtcggt tcccgtctct tttgccaggt cacgcaccca
      301 ccacggctcg tcaccaaaca gcggctctgc tttttgccag atggcctgct gctcttcgct
      361 gaagcccgct ttgtgatccg gcagatgcag ccaaccgtga tgactgagga tgtcgccgct
      421 ttcgcgcatc ttttcaatca gcaacagtac cagcgcttca tcttccattg gcaacgccat
      481 acgtcgcaga cgttcgcgcc caggaccagg ttcatcacga tgttgctcat gataagtcgc
      541 taatgtgtcg agaattttcc gctgccagcg ggcggcaacc ggcgcattca acaagctata
      601 accagcctga atatagccag gctgttgcag cagttcgcgc atcccttcgc cgttgagctg
      661 gcgcgcccag gcgaaatccg caaggttaac cgctccgcgt tccagatgaa cagataacgc
      721 atcggcatcg ctctgcgccc gtgcaagaga cgccagccat tgcagatatt ccggcttacg
      781 tttaccgcga cgcggcgggt taagcatcac cacacgcgcc cccgccagcg tattgcgggc
      841 cgagatatcg cgcaacacta agcgatcgtt atccgccagc cacagcggcg tgtcgaacac
      901 cagctcggca aggttatctt ccagcagcga aacgcgtccc gtaacgtggc tggcggcgtg
      961 gtgaatatgc agcggctgcc actgggtcag cggcgtatgg gtttgaagct cgacaatcac
     1021 ccgcgtgaac ggctctggcg gcacatcggc aagcagccag tcgccacggt taatctgctc
     1081 tttttccgca tcacccgcga tgttaagcgc gatacgctgc ccggcatggg cggtttctgt
     1141 tggctggttt tgtgcatgca gcgcacgtac acgcatcggt ttatttacac cagttagcca
     1201 tagtgaatcg cctaccttca cttccccgct taacgccgta ccggtgacga ccagcccggc
     1261 accttttacg gtaaatgcgc ggtcgatcgc gaggcggaaa ctatgttggc tagcgtgttc
     1321 acgttctggt aactgaagca gatgctcgcg cagggcatca attccccgac cttcggttgc
     1381 tgcggtgata aacagttttg cctcagcaaa accgtattcc cgcagcacct cctttacctg
     1441 gcgttcaacc tcatcaacac gcgcttcgtc cacgcgatcg gctttggtca gcgccactgt
     1501 cagcatcggg ttaccagtca gctgcaaaat cgccagatgc tcacgggtct gtgccatcac
     1561 gccgtcatcg cacgccacca ccaacagcgc gtgatcgata ccaccaacgc ccgccagcat
     1621 gttggaaaga aacttctcat gaccgggtac gtcgataaaa ccaggcacgc gaccatccgg
     1681 ctgcggccag taggcatagc cgagatcgat agtcatgccg cgcttttttt cttccggcag
     1741 acgatcggca tttacgccag taatcgcctg caataaggtt gttttgccgt ggtcaacgtg
     1801 tccggcagtc gcaataatca tttcaacaac atctccaaaa accgttgctc atcttcaagg
     1861 cagcgtaaat ccagccacaa tcgtccgtca taaatacgac caatcaccgg cactggcaat
     1921 tcacgccagc gggcggctaa tgactcaagg tggctaccgc gtccatcatg gggtgtaaac
     1981 gttaatgccg cgctcggcag gcgatcaacc ggcagcgaac cactgccaat ctgcgaaaga
     2041 catggcataa cctgtaccgc aaactccgcg ccgtaatgtg cggcaagggg agcctgtaaa
     2101 cgttgtgcct ggatttgaat gacctctgcg ctgcgggtaa gcaggcgcag ggtcggtaat
     2161 ttttcactca gagcttcagg gtgtaaataa agacgcaacg tggcttccag cgccgcaagg
     2221 gtcattttat ccgcgcgtaa tgcacgcttc agcgggtggc tttgcaggcg ggcgatcatc
     2281 tcttttttac caacgataat ccctgcctgc ggcccgccta acaacttgtc gccggagaag
     2341 ctcaccagac tgacgcccgc cgcaatcaac tcctgcggca ttggctcttt cggcaaaccg
     2401 tactggctaa gatcgaccag cgagccactg cctaaatcag tcactacggg aacatccagc
     2461 tctttgccga gcgccaccag ttcagcttcg tctatcgctt tggtgaaccc ctgaatgctg
     2521 tagttactgg tatgtacttt catcaacagt gcggtatttt cattcaccgc ctgacgataa
     2581 tcattcgcgt gcgtgcggtt ggtggtccct acttcgtgta gggtgcagac tgcctgacgc
     2641 ataacatcgg gaatacgaaa cgcgccgcca atctccacca gttcgccgcg agaaaccacc
     2701 acctctttcc cgctggcagt ggccgccaac atcaataaca ccgccgccgc attgttatta
     2761 acgatacagg catcttccgc gccggtaatg cggcacagca gctgcgccag cgcccgatcg
     2821 cgatgtccgc gcccggcgtc gtccagatcg tactcgaggg tcactggcga acgcatagcc
     2881 tgcgcaacgg cttccaccgc ggcttccgcc tgtaaagctc gcccaaggtt ggtatgcagc
     2941 acggttcccg tcaggttgat caccggacgc agcgcgctct gcgcctcttt cgtcaaccgg
     3001 gcatcgactt cttgcgccca gttttcacac cacgtgggaa gcgtctggct gtcacgaatc
     3061 acttctcgcg cttcgtcgag catctgacgc aacaattcca ccacgcgggt gtgaccataa
     3121 gtatcacgca aagaaaggaa ggagctatcg cgcaataagc gatcaatagc cggaagttga
     3181 ctatagagag aacgcgtttc ggttgtcata ggaaacctgg ctgatcaagg ccctctcaca
     3241 cggagaaggg cgtttaacat aaccacggat tgtaacgtga gatgggtcag gaggacatat
     3301 cgcgcatcaa gcctttggcg gttcggtgcg agcaaaactt tcgcggctaa acaggttttc
     3361 gaccagtttg actaagtgtg ggcggtcgac acaccagcct ggcgcaacgc ggcggaaatt
     3421 gagatatccg acggcacagg caatggcgat agtcgccaga ttgaccgtat cggttttgag
     3481 tgtgccatcg accagatatc cttccagcac atccagactg cggttgattt tctcccgctg
     3541 gcgtaacaat tcatcttcag actgctgcgc cgctggacgc gcctgttcac gcaccgatac
     3601 cagcccggca tccataatgc catccgccag tgcctcaatt ttgcgcaccc gcaacgactc
     3661 cagcggatcg cgcggcaaca tcgccggagc gacattcatt aattcaatat attcagcgat
     3721 gatcggcgaa tcaaaccagc attcgccctc ttcggtcacc agcaccggca cttttcctaa
     3781 cgggttaaat tgcgccacgc cgttgtccgc gttatagggc agctcattaa taaattcgaa
     3841 agttatgccc ttttctaaca acagaataga aagtttgcgt acaaacgggc tggtgtagct
     3901 accgacgagt ttcatgccga gtcctttgtg cgaggaaaaa tatcagtatg gctcaggctc
     3961 acgcaaaaca caggtgatat aagtctcaat gatccaaata aagataatgc atccagctgg
     4021 tcattcttag cagcactttt cgcatcaccg aaacatggct gaaatggtcg gagtaaactg
     4081 ccacctgggc gtaaatgccg tcgggtaagg catcgatatc atcgttaggg tccagttcaa
     4141 tggttcccag cacaccgtcc gtgccgggca caaccgttaa tgattgcaat accccctgcg
     4201 cctgataaga accgcctggc acgacaggta aaatactggt cagtttgccg tggaacacct
     4261 gcccaggtag cgcgttaaac accacttccg catcatcacc aggtttcaga cgtaacagcg
     4321 agttttgccg aaattgggcg acaatttgcc gtttttgctc ggggatgaaa accatcaccg
     4381 gacgcaacgg caaggcagcg gcgtatgtgc ctgggcggat cagtacctga gtgacgtagc
     4441 cattgcttgg tgcgcgaatg acagtctgct caagattata ttttgcttca gtaagttgcg
     4501 ctcttaagct cacaatctgc gattgctcgc cgttgaccat actgtcgagc tgactctgga
     4561 tctgcgcctg ctccgccacc gagcctttca ctaacgcatc ctgcgcgagg aaattttgcc
     4621 gcgcatcgtc gatgtcacgt tccgagaacg gattcaccgc cgcctggctg cctttcaggt
     4681 aacgttgata atttttaaac agacggtcgc gttccgctga aacctgggtg gtgttggcct
     4741 gcgcttcagt gagctgcgcg cgcagcgtct ttatattatg cgtcgccgtc atcaggtcag
     4801 cctgaagtct gtcaactcgc gcctggtaac gaaccgggtc gagcttaaaa agcacctcgc
     4861 ccttttgaat aagctgatta ttcttgtcag tgacttcagt aacaattccc gtcacctgtg
     4921 gcgtgatggg gatcgctatc actgcctttt gcgcggtaaa agtgtaaggg tggttgtagt
     4981 tcatcaacaa aatcaaacca ctcaccagaa acacgcctcc cagcgccgcc gtcgccagcg
     5041 tccactgatt caccggaatg cggaagattt taaagaccgc ccacgccagc gccacgtaag
     5101 ttaaaacaat caatagatcc ataattagat ctccgggaac gtggaataat cagtcttttt
     5161 ctcggcggcg agttggtgct ccagcctgga aattcggtca gaaagtgcgg cgatttccgg
     5221 gtcagtcact ttctcctgcg acgcaacatg tgactgcatg ccccagccgc gctccggttg
     5281 atagagcgtc gcccagatcc acagaaacgg ccagataaca tgcagagtaa acaggctcac
     5341 ccagcccgcc gtatgaatag cgtcggcatg gggatggttg cgctttttgg caatcaaata
     5401 agggatgtca tgtatcgcga tgattccata aaaaatcacc agaaagacga agatcagcac
     5461 tcccaacgcg aaatagttta gaaacatatt tgcctcggat tcacgtttat cagtgttgtt
     5521 ttttggctgg cagccagaag ggagtcaggc tgatattttg acaataatcc gggttcgcga
     5581 ttctcgccat aacaccaaag aataattttt agaggtgatg agttgcttag ttacataacg
     5641 attgtatgac gaaggcataa catgctgtag atcacatcag gtgaacgccg taagaaaata
     5701 tcttgtgatt cagatcacaa agattcaaca aaccatcaaa acaaaaatgt gacactactc
     5761 acatttaaat gccattttta gcgaaaatcg ccgccttgtt gcttttttac acaagcgttt
     5821 tgtgatgaac gtcacgtcaa ttacctctct accccctata tttatgtgat tgatatcaca
     5881 caaaaggccg tcgactggac agttaaccga ttcagtgcca gatttcgcag tatctacaag
     5941 gtccggctac ctctgccgcc acattaacaa aaaacctcgg gcttccagcc tgcgcgacag
     6001 caaacataag aaggggtgtt tttatgtcat ccgatattaa gatcaaagtg caaagctttg
     6061 gtcgtttcct cagcaacatg gtgatgccaa atatcggcgc gtttatcgcg tggggtatca
     6121 tcaccgcgtt atttattcca acagggtggt taccgaacga gacgctggcg aagctggtcg
     6181 ggccgatgat cacttatctc ctgccgctgc tgatcggtta taccggtggt aagctggtag
     6241 gcggcgaacg tggcggcgta gtcggtgcca tcaccaccat gggcgttatc gtcggcgcag
     6301 acatgccgat gttcctcggt tctatgattg caggtccact gggcggctgg tgcattaagc
     6361 acttcgaccg ctgggtagac ggtaagatca aatccggttt tgagatgctg gtgaataact
     6421 tctccgcagg catcatcggg atgatcctcg ccattctggc atttctcggc attggcccga
     6481 ttgttgaagc cctgtccaaa atgctggctg cgggcgttaa cttcatggtt gtccatgaca
     6541 tgctgccgct ggcgtctatc tttgttgaac cggcgaaaat cctgttcctc aacaacgcca
     6601 ttaaccacgg tatcttctcg ccgctgggta ttcagcagtc ccatgaactg ggtaaatcca
     6661 tattcttcct gattgaagct aacccaggtc cgggtatggg cgtgctgctg gcgtacatgt
     6721 tctttggtcg tggtagcgct aaacagtctg cgggcggtgc ggcaattatc cacttcctgg
     6781 gtggtatcca cgaaatttac ttcccgtatg tgttgatgaa tccgcgtctg atcctggccg
     6841 ttatcctcgg cggtatgact ggcgtgttca cgctgactat cctgggcggt ggtctggttt
     6901 ctccggcatc tccgggttct atccttgctg tactggcgat gacaccaaaa ggtgcttatt
     6961 tcgctaacat cgcgggtgtg tgtgcggcga tggctgtctc ctttgttgtc tctgctattt
     7021 tgctgaaaac cagcaaagtg aaagaagaag atgatattga agcagcaact cgtcgtatgc
     7081 aggacatgaa agctgagtct aaaggtgcat ctccgctgtc tgcgggcgat gtgactaacg
     7141 acctgagcca cgtacgtaaa atcatcgttg cctgtgacgc cggtatgggt tcaagtgcga
     7201 tgggcgcagg cgtgctgcgt aagaaaattc aggatgcagg tctgtcgcag atttctgtta
     7261 ctaacagcgc gatcaacaac ctgccaccgg atgtggacct ggtcatcact caccgtgatc
     7321 tgaccgaacg cgctatgcgc caggttccgc aggcacagca tatttcgctg accaacttcc
     7381 tcgacagtgg cctgtacacc agcctgactg aacgtctggt tgctgcccag cgccatactg
     7441 aaaacgaagt gaaagtgaaa gacagtctga aagacagctt tgacgattcc agtgctaacc
     7501 tgttcaaact gggcgcggag aacatcttcc tcggtcgcaa agcagcaacc aaagaagaag
     7561 cgattcgttt tgctggcgag cagctggtga aaggcggtta cgttgagccg gaatacgttc
     7621 aagcgatgct ggatcgtgaa aaactgaccc cgacttatct gggtgagtct atcgcggtgc
     7681 cacacggtac ggttgaagcg aaggatcgcg tactgaaaac gggtgtcgtg ttctgccagt
     7741 acccggaagg cgtgcgcttc ggtgaagaag atgatgacat tgcccgtctg gtgattggta
     7801 ttgctgcccg caacaacgag cacattcagg ttatcaccag cctgaccaat gcactggatg
     7861 atgagtccgt catcgagcgt ctggcacaca ccaccagcgt ggatgaagtg ctagaactgc
     7921 tggcaggtcg taagtaatcc aatcccaccc tctccacatg gagaaggtgg ggttaattgc
     7981 ctgatgcgct acgcttatca ggcctacagg atgcatcacg atttgttgaa tttgcacgtt
     8041 cttgtaggcc ggataaggcg cttacgccgc atccggcgct gcccctctcc tcacggagag
     8101 ggtttgggtg agggaaaagc ctcaccccag ccctctcggg taaaaacatt gatgaaggtt
     8161 aatactatga aagcattaca ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa
     8221 ctgctggcag acgcgggtat acaactgacg tttgccgatg tcaatcaggt ggtacttgat
     8281 gccctgaatg cccgtcatag ctatcaggtt catgtggtag gtgaaaccga gcaggtagat
     8341 accgtttccg gcgtcaatgc tgtcagcagc attggtgatg atgtcgttga tctcattgct
     8401 caggttgatt tagtcactac cgccgttggc ccggtagtgc tggaacgtat tgctccggct
     8461 atcgccaaag ggctggtgaa acgtaaagaa caaggtaatg aatccccgct gaacatcatc
     8521 gcctgtgaaa acatggtacg cggtaccacg cagctgaaag gccatgtgat gaacgccctg
     8581 ccggaagacg ccaacgcgtg ggtagaagaa cacgttggct ttgtcgattc cgccgttgac
     8641 cgcatcgtac cgccttcggc ttcggcaact aacgatccgc tggaagtgac ggtagaaacc
     8701 ttcagcgaat ggattgtcga taaaacgcag ttcaaaggtg cactgccgaa catcccaggc
     8761 atggagttaa ccgacaacct gatggcattt gtcgaacgta aactcttcac cctgaacacg
     8821 ggtcatgcta taaccgcgta cctcggaaaa ctggccggtc atcagaccat tcgtgacgct
     8881 attctcgacg agaaaatccg cgcggtggta aaaggtgcga tggaagaaag tggcgcggta
     8941 ctgatcaagc gttacggctt tgacgctgac aagcatgcgg cgtacatcca gaaaatcctc
     9001 ggccgttttg agaacccgta tctgaaagat gatgtagagc gcgtaggccg tcagccgctg
     9061 cgtaaactga gcgcaggcga ccgtctgatc aagccactgc tcggtacgct ggaatatggt
     9121 ctgccacata aaaacctgat tgaaggtatt gccgctgcaa tgcacttccg cagtgaagat
     9181 gatccgcagg ctcaggaact ggcagcactg atcgctgaca aaggtccgca ggcggcgctg
     9241 gcacagattt ccggtcttga tgccaacagc gaggttgtat ctgaggcggt aaccgcttat
     9301 aaagcaatgc aataatggtg gaccaggcgc aggacaccct gcgcccgaat aacagattgt
     9361 cagatatgca ggcaacaatg gaacaaaccc aggcctttga aaaccgtgtg cttgagcgtc
     9421 tgaatgctgg caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctc ctgaccgagg
     9481 cggtaaatct tctggtgctt caggtattcc gcaaagacga ttacgcggtg aaatatgctg
     9541 tagaaccgtt actcgacggc gatggtccgc tgggcgatct ttctgtgcgt ttaaaactca
     9601 tttacgggtt gggcgtcatt aaccgccagg aatacgaaga tgcggagctg ctgatggcat
     9661 tgcgtgaaga actaaatcac gacggcaacg agtacgcctt taccgacgac gaaatccttg
     9721 gcccctttgg tgaactgcac tgcgtggcgg cgttaccacc gccgccacag tttgaaccag
     9781 cagactccag tttgtatgca atgcaaattc agcgctatca acaggctgtg cgatcaacaa
     9841 tggtcctttc actgacagag ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt
     9901 aagcaacgtc tgcttactgc ccctctacct gcttcggccg ataaagccga cgataatact
     9961 ccagacgttg tagatataac ggcacgcttt catcgggtat tccggacgga atcgcgttac
    10021 ggggaggaag ttttttcaga tactcccgga acgcctggct tgatgccatg aaatctacgg
    10081 ctttatcgat aagaagcgga acgttttcac ctaatttgcc catgatgata tctccgtatt
    10141 accccgcgcc gggaatgcgc ggccgccaat tttagtttag gttcccggta aagatgacat
    10201 taaggaaaac gtgctgaatc ctcaataatc atccgcttct gtttattatc ttttttctat
    10261 agatttctta attaatcaat aaattgcgta gtttttaagc atattaatga ttacgttgcg
    10321 gaactattgc aatgtcatca gttgtgaaca aagcacctgg tcgcgcatac taggggctat
    10381 aaatttatct ttatcagaag ccatcacatg aaagaagtcg aaaaaaacga aatcaaacgt
    10441 ctcagcgatc gtctggacgc catccgccac cagcaggccg atctgtcgct ggttgatgcc
    10501 gcagacaaat atgccgagct ggaaaaagag aaagccacgc tggaagcaga aattgcccgc
    10561 ctgcgtgaag ttcacagcca gaaactgagt aaagaagcac agaagctgat gaagatgccg
    10621 tttcagcgcg caattaccaa aaaagagcag gctgatatgg gcaagctgaa gaaaagtgtt
    10681 cgcggactgg tggttgttca cccaatgacc gcgctgggcc gcgaaatggg cctggaagag
    10741 atgactggat tctctaagac tgcgttttaa gcccctcaga cgattggtac gatcgtatca
    10801 acccgcatct ggcttgcgtt agctggtcaa tgcggggact cccctgtctg ctcgcctaat
    10861 tactgaacaa tccactccct ctacatcaat ttaccgctgg ttgatattct gaaaatatat
    10921 ttttaccgaa
//
LOCUS       DAZUCE010000173        10880 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZUCE010000173 DAZUCE010000000
VERSION     DAZUCE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.173"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            30..827
                     /locus_tag="ACHY03_003760"
     CDS             30..827
                     /locus_tag="ACHY03_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFS3906942.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            838..1662
                     /locus_tag="ACHY03_003761"
     CDS             838..1662
                     /locus_tag="ACHY03_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFS3906943.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHTADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            1714..2943
                     /gene="sorE"
                     /locus_tag="ACHY03_003762"
     CDS             1714..2943
                     /gene="sorE"
                     /locus_tag="ACHY03_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFS3906944.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSCPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            3325..4014
                     /gene="pepE"
                     /locus_tag="ACHY03_003763"
     CDS             3325..4014
                     /gene="pepE"
                     /locus_tag="ACHY03_003763"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFS3906945.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(4104..5735)
                     /locus_tag="ACHY03_003764"
     CDS             complement(4104..5735)
                     /locus_tag="ACHY03_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFS3906946.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(5955..9638)
                     /gene="metH"
                     /locus_tag="ACHY03_003765"
     CDS             complement(5955..9638)
                     /gene="metH"
                     /locus_tag="ACHY03_003765"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFS3906947.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARTCADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEVAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRAWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAREVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            9838..10662
                     /gene="iclR"
                     /locus_tag="ACHY03_003766"
     CDS             9838..10662
                     /gene="iclR"
                     /locus_tag="ACHY03_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFS3906948.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2460 a         2986 c         2874 g         2560 t
ORIGIN      
        1 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa attattgcta
       61 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattc cagactcatc
      121 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
      181 tactcggtgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
      241 ctccggactc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
      301 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
      361 tgtttgcacg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcggaagagg
      421 cacggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
      481 ttgctattcc ggcactgatt gtctcgctgt tcgtcagcgc cgatatggtc agcaatatgt
      541 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
      601 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
      661 tcctcggttt cctcgcaggt ggctacctcg atctcagtct gctggcgttc ggtggcgtcg
      721 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
      781 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
      841 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
      901 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
      961 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     1021 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgtcac tgccgccatg
     1081 gaagaggcgc gggccaacgg cgcggaaatt gatgacggaa ccattaacgg catcaaagtc
     1141 ggtctgatgg gaccattggc aggggttggc gatccactgg tctggggaac gctgcgccct
     1201 attaccgccg cgctcggcgc atctctggca ctttcgggca acattctcgg cccgctgctg
     1261 ttctttttta ttttcaacgc ggtgcgtctg gcgatgaagt ggtatggcct acagctcggc
     1321 tttcgcaaag gggtgaatat cgtcagcgat atgggcggga atgtgctgca aaaactcacc
     1381 gaaggcgcgt cgattctcgg actgtttgtg atgggcgtgc tggtcaccaa atggacgtca
     1441 atcaacgtac cgttggtggt ttcacaaacg cataccgccg atggctccac cgtcaccatg
     1501 accgtgcaga acattctcga ccaactttgt cctggtttgc tggcgctcgg tctgacgctg
     1561 ctgatggtgc gtctgctcaa caaaaaaatt aacccggtat ggctgatttt cgccctgttt
     1621 ggcttaggga ttatcggcaa tgcgctgggc ttcctgtcct gattcttcgc cccggcacga
     1681 ctgccggggc catcgctcaa catgaggtgg tttatgaaaa caacagctct gcgtctttat
     1741 ggtaaacgtg atttacgcct ggaaaccttt gaccttcctg aaatgcagga ggatgaaatc
     1801 ctcgcgacgg tggtcactga cagcctgtgc ctctcttcct ggaaagaggc caacctgggt
     1861 gaaaaccata aaaaagtacc cgacgatgtg gcgaccaacc caatcatcat cggccacgag
     1921 ttttgcggcg atattctggc cgtgggtaaa aagtggcagc acaaattcca gccgggtcag
     1981 cgttatgtga ttcaggccaa cctgcaactc cccgaccgcc cggactgccc cggctactcc
     2041 tgcccgtggg tgggtggtga agccacacat gtggttattc ccaacgaggt catggaacaa
     2101 gattgcctgc tggcatacga cggcgaaacc tattttgaag gctcgctggt tgaaccgctt
     2161 tcctgcgtga ttggcgcgtt caacgccaac tatcatcttc aggaaggtag ttataaccac
     2221 acgatgggga ttcgcccgca agggcgcacg ctgatcctcg gcggcaccgg accaatggga
     2281 ctgttggcga ttgattatgc gctacatgga cccgttaacc cgtcgctgct cgtcattacc
     2341 gataccgaca acgataaatt gagttatgcg cgcaagcact atccatcaga accgcaaaca
     2401 ctgattcatt atctcaatgc caccgatgca gcatttgata cgctaatggc gctgagtggc
     2461 ggtcacggct tcgatgatat tttcgtcttt gtgcctaatg aaggactggt gactctcgcc
     2521 tcttccttgc tggcgacaga tggttgcctg aatttcttcg ccggaccgca ggataagcac
     2581 ttcagcgcgc caattaattt ctacgatgtg cattatgcct ttactcatta tgttggcaca
     2641 tcgggcggca ataccgatga catgcgcgcg gcggtcaaac tgatcgaaga gaaaaaagtg
     2701 caggccgcaa aagtggtaac acatattctt gggctgaatg ccgcgggcga aaccacgctt
     2761 gaattgcctg ccgtcggcgg cggcaaaaag ctggtgtata ccgggaaata cctgccgctg
     2821 acgtcactca cgcagattca ggatcaagca ctggcggcga ttctggcgcg tcatcagggg
     2881 atctggtccg gtgaggcgga gcaatatctg ctcactcatg cagaggcaat ttcccatgat
     2941 taatcgcact actcaactct gcatgtcgct ggctggtcgc cgcggcaact tcgcgacctg
     3001 atgtgcggct gattgctgaa gcggtccaat gtgcacgaga aggataaaaa aacatcagcg
     3061 ccttaagggg cgctcaaatt ggtggcaaag tgcacattgt ttatgccgga tgcggaatga
     3121 agccttattc ggcctacata atctcgaaaa ttcaatatat tgcagttatc tggtaggcct
     3181 ggtaagcgta gcgcatcagg caatgctgtg tttgtcatca gtctcagcgc ctgtgcaata
     3241 cgtgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca ttccttccgt
     3301 gatttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc gacgctgccg
     3361 ggtaaagcct ggctggaaca tgcactgccg ctaattgctg aacagttgca gggtcgccgc
     3421 tcagcggtgt ttatcccttt cgctggcgta acgcagacct gggatgatta cacagagaaa
     3481 acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat
     3541 cccgttgccg cgattgaaaa tgctgagatc gtgattgtcg gcggcggtaa tactttccag
     3601 ttgctgaaac agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt ggttaaacgt
     3661 ggcgcactgt atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc
     3721 accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctgaatct gttcccgttg
     3781 caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgaa
     3841 cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctaccggaa
     3901 ggtaactgga tcacagtgag taaaggtcac gcaacgctgg gtggcccgaa caccacctat
     3961 gtgtttaagg ccggtgaaga agcggttccg ctggaagcgg gtcaccgttt ttaagtgaat
     4021 tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt tacgcgtctt
     4081 atcaggccta cagggtcacg gtattaatac tcgtcccgtc cttcatcttc atccggctgt
     4141 tccagcacac tgtaagccac cgaacaaaac agcgagttca gacgctgcat atctcccagt
     4201 aagcctaaat gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg
     4261 acgtgggcgt gggaatagcg gcgattaaga atgcgaaagc gatgcttgct gcggcgcaaa
     4321 cgacgggcgc tggtaacatc accagagaag aaaaccgaca ttgccagttt taaattactg
     4381 agcaattgct catagagcgc atccagttcc ttcaagccat caagcgaaaa tgcccgccgt
     4441 gctgccagcg atttatcggc aatttcgctc cccatgcgct cgacgatatc ggaggcctgt
     4501 tcaaggttga gcgacatttc gatgatctcc gcccagcggc gcgactcttc ctccgccagc
     4561 tcttcttttg gcatccgcgc cagatacagc ttaatggcgg tatagagaac gttgatatca
     4621 tctgccagct tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc
     4681 ccttccatca tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc
     4741 agagcaagcg tgggcgtatc cagcgcgctg acatccagat gtttgggccg tagctgggta
     4801 tccagttccg gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat
     4861 ggcagcatga ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc
     4921 gcttttggca atgacaactt ccccattgtc tctgccagca aatggacaaa cggcaggata
     4981 atcaggctac ccaccagctt aaacagcaaa ctacccagcg cgacacggcg ggctgcggca
     5041 ttggcggcac tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc
     5101 agacagagcg ccacggggaa ggagataatg cctgcggcgg tcagagtcgc agtcagcagt
     5161 acagcagcaa ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc
     5221 agaatatcgc cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg agtgatcggc
     5281 gttacggcct gcacaatcaa ctccagcgcc agtaaaatca gcccaagacc aataccgacg
     5341 cggcccagtt gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa
     5401 ataagtaacg gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc
     5461 ccgacatctg cacccagcac aatgaccaga gccggtgcga gggctaccag atcctgagcg
     5521 acaaacgagg tcaccagcat ggtggtggca ttactgctct gtaccagtgc ggtaacgccg
     5581 atccccgcgc aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaga
     5641 cgcgcgccga agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg
     5701 gcgacggcag aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat
     5761 atgttcctga agcaattgaa taaccaaagg accggaaaca cgctgactgg catctttact
     5821 gtgcctttta acattcgttt ttccggctaa gattattcca gcctgattcc agtataagtg
     5881 tttcatatta ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt
     5941 gacagatttg tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca
     6001 tcggagacgc tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc
     6061 gccaccgcgt agtacttgct gtccggatga ctgaaatacc aacccgaaac cgatgcacca
     6121 ggccacatgg cgaaagattc tgtgagtttc atgccagtat gtttttccac ttccagcagc
     6181 tcccagatgg tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg
     6241 atgccctggt agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag
     6301 ccccagtaga ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg
     6361 tcggcaagcg ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca
     6421 gccagtgcgt cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta
     6481 ccagaaagct tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg
     6541 gtctgttgac gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag
     6601 atttcaatgt catcgcccac acggtttgcc gggaacagcc ccaccacacc acgcggattc
     6661 agcgttttct cagcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc
     6721 gcctcaacgc ctaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag
     6781 gtcataaaga acggcgtcca gtcgatgtaa ttacgcagcg tttcgatgct ggcttcaact
     6841 tcctgcacac cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaaa
     6901 tcgttatcgc gcgccgcttc cagcgtgacc ggtggtgtgc gtggtttctt gcgcccgtgc
     6961 tgaatacgca cggtttcgta ctccttgcgg gtacgagcaa cgaaatcatc gcgctgagtg
     7021 tcggaaagca gcgccgccac cacgccaacg gtgcgcgagg cgttctgcac atatactgtc
     7081 gggccgctgt agttctgctc gattttcacc gccgtgtgcg ctttcgaagt agtcgcgccg
     7141 ccaatcagga gtggaatggt gaagccttga cgctccatct ccttagccac gttaaccatc
     7201 tcgtccagcg acggcgtgat cagacccgag aggccaatca gatcagcatt cacttctctg
     7261 gcggtgcgga ggattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg
     7321 ttacattgca gcaccacacc aacgatattt ttaccgatat cgtggacgtc gcccttcacg
     7381 gtggcgatca ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt
     7441 tcgaggtagg ccaccgcctg cttcatcaca cgcgccgatt tgaccacctg tggcaggaac
     7501 attttccctt cgccaaacag atcgccgacc acgttcatgc cgtccattag cgggccttca
     7561 atcacttcaa tcgggcgcgt agcctgctgg cgtgcttctt cggtatcctg ctcgataaac
     7621 tcggtaatgc ctttgaccaa cgagtactcc agacgtttat tcacttccca cgcgcgccac
     7681 tccgcctgct gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc
     7741 tccagtaaac gctcggtgcc atcgtcgcga cgattaagaa tcacatcttc caccgcgtcg
     7801 cgcagttccg cgggcaggtc gtcgtaaatc gccagctgcc cggcgttgac gatccccata
     7861 tccatgccat tgcgaatagc gtagtagagg aacaccgcgt gaatagcttc gcgcaccgga
     7921 tcgttaccac ggaatgagaa agaaacgtta gatacgccgc cggaaatcag cgcgtgcggc
     7981 agttcgcgtt tgatgtcttc gcaggcgccg ataaagtcct gcgcatagtt gttgtgctct
     8041 tcaatgccgg tggcgaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag
     8101 ccaacctctt cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg
     8161 gtatcggcct gtccctgttc gtcaaaggcc attaccacca ccgctgcacc gtaacgacgc
     8221 aacagtttcg catgatggat aaaggcgtcg acgccctctt tcattgagat agagttaaca
     8281 atgcctttgc cctgaataca cttcagcccc ttttcgatga cctcccattt tgaggagtcg
     8341 atcataatcg gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc
     8401 atcgccactt cggcgtcgag catcccttca tccatattga tatcgataat ctgcgcaccg
     8461 ttttccacct gctggcgggc cacatccagc gcctcgctgt atttctcttc tttgatcagg
     8521 cgcttgaact tagcggaacc ggtgacgttg gtgcgttcac ccacgttcac aaacaggcta
     8581 tcgtcgccaa tgttcagcgg ctccaggccg gacaaacggc aggctacggg aatttcaggc
     8641 agtttgcgcg gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg
     8701 gtgccacagc agccgccgac gatattgaga aaacccgctt gcgcccattc acgtatctgt
     8761 tttgccatcg tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc
     8821 gggtgcgcgg tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt
     8881 tcatctggcc ccagcgcgca gttgaggcca aaagtcaggg cttcggcatg gcgcagggag
     8941 ttgtaaaatg cttcggtggt ctgtccggag agcgtgcgcc cggaggcgtc ggtgatggtg
     9001 ccggagatca taatcggcag ctcaacgccc agcgcttcaa actccgtttt caccgcaaag
     9061 accgccgctt tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca
     9121 ccttccacca gcgctttggt ggattcgcga taggccgcca ccagctggtc aaaagtgatg
     9181 ttgcggaatg ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggtcgggccg
     9241 agaacaccgg caacgtagcg cggtttctcc ggcgtgcgcg cggtccactc gtcagcacag
     9301 gtgcgcgcca gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa
     9361 tccgccatcg caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct
     9421 tcaaagtagg cgttgtggat agcggcgatc acttccggtt tgcttagcac cagcagatcg
     9481 ttattgcctt tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg
     9541 ttcagtcgat agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca
     9601 tttaactgcg cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac
     9661 ccagatttaa taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga
     9721 ccaccacgca acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg
     9781 cattagctaa caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg
     9841 gtcgcaccca ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact
     9901 ggacaggttc agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat
     9961 ggcagtgtgg cactcacaga gctggcgcaa caagccgggt tacccaattc cacgacacac
    10021 cgcctgctaa ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac
    10081 tgggcaatcg gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg
    10141 ttagcaattg ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat
    10201 atggcggtgc ttgatcaaag cgatcacgaa gcgattatta tcgaccaggt acagtgtacg
    10261 catctgatgc gaatgtccgc gcctatcggc ggtaaattac ctatgcacgc ttccggtgcg
    10321 ggtaaagcct ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa
    10381 gggttacatg cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc
    10441 gcccaaacgc gcaaacgggg ttattcattt gacgatgagg aacatgcact ggggctacgt
    10501 tgccttgcag cgtgtatttt cgatgagcac cgtgaaccgt ttgccgcaat ttctatttcc
    10561 ggaccgattt cacgtattac cgatgaccgc gtcaccgagt ttggcgcgat ggtgattaaa
    10621 gcggcgaagg aagtgacgct ggcgtacggt ggaatgcgct gactttttct ggcgggcaga
    10681 ggcaatattc tgcccatcat acctgagtgg caatagaata agggtgtctg ttaatcgcat
    10741 tgacgccaaa ataacttaat gtcatacact tcacacactt ttattaactc agcattattt
    10801 ttaaacatca aaccacttaa ttattaacaa aatcgcaaga tctcattcca ccgaaacttt
    10861 tacggctgca ttatgagatt
//
LOCUS       DAZUCE010000174        10875 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZUCE010000174 DAZUCE010000000
VERSION     DAZUCE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.174"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(371..460)
                     /locus_tag="ACHY03_003767"
     tRNA            complement(371..460)
                     /locus_tag="ACHY03_003767"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Ser,seq:cga)
     gene            554..1351
                     /gene="mtfA"
                     /locus_tag="ACHY03_003768"
     CDS             554..1351
                     /gene="mtfA"
                     /locus_tag="ACHY03_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFS3906949.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1452..1527
                     /locus_tag="ACHY03_003769"
     tRNA            1452..1527
                     /locus_tag="ACHY03_003769"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1485..1487,aa:Asn,seq:gtt)
     gene            1841..9823
                     /locus_tag="ACHY03_003770"
     CDS             1841..9823
                     /locus_tag="ACHY03_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFS3906950.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLVTQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AENMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSTQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLADENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPPGEITVTDTAPQQLTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNGGKGMTDSNGVAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVYTAEATLPNGNNDTKIVNIAPDASNALVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPLAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTLKGTVLGVHT
                     VEATLLNGNGYTTTVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTLSNNGINTTNHDGYLYASF
                     TATKAGVYQVTATLDNGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTAS
                     ITGGKSEPLVVNFTADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVRGAITGSVTVSAVTTAGGMQTVDITLVAGPADASKS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNVPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSNWER
                     ITATPKSGGPSYVYEIRVKSWWVNAGEAFMIYSLAENFCSSNGYTLPRANYLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFQSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(10085..>10875)
                     /locus_tag="ACHY03_003771"
     CDS             complement(10085..>10875)
                     /locus_tag="ACHY03_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFS3906951.1"
                     /translation="QDHATLKEIKDLAKNITQQYGTPDLCIEAMRKKNLKTMLFISQL
                     KAKTNFQVVGLTMKCYSPACKNASRMDQNLRAPVPMTWAFMMREAGFPAVRPIYELPL
                     SEDVLDEVREEMRSLGSYIALNLEGSSQERTFSLLIAENLIAKIQSETDIPIVIVHGP
                     KGEGKARALVDRYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVA
                     IYADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         3018 a         2630 c         2629 g         2598 t
ORIGIN      
        1 tcaaaaaagc tgggaaattc aacaactctt ggaaattatg gactttgtcc cgcggcttta
       61 atctgacggc cgcgttcctt ttttgactca attattgacc ccttctctac gcaacttcgg
      121 ttcccaccac caactttgcg gcagtttcat aggatgaatg tctaaaagaa taatggtgag
      181 cgataagaaa acgactgaat aactgcacat tttcgctcga aagcttcctg tcagatccat
      241 agcgaatcaa gtgctgaatg tcacagtatc gaacagaaaa cagtgacgat ctaacccttc
      301 aagaatattc tacgattgtt ctgtttagga aaagcaaggc gggaagtcgg gagataagtc
      361 attgataaag tggcggagag agggggattt gaacccccgg tagagttgcc cctactccgg
      421 ttttcgagac cggtccgttc agccgctccg gcatctctcc gttcagatgg ttgccatgat
      481 gccaggaaat ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa gtgacgagtt
      541 tgcgagcaaa acgatgatta agtggccctg gaaagtacaa gaatcagcac atcaaactgc
      601 ccttccctgg caggaagcac tatcgatccc ccttttaacg tgtctgacgg aacaggaaca
      661 aagcaaatta gtcactcttg ccgaacgttt tttacagcaa aagcggcttg ttcctttaca
      721 gggctttgaa ctggattcat taagaagctg ccggatagca cttctatttt gcctacccgt
      781 tctggagtta ggactggaat ggctggatgg ttttcatgaa gtcttaattt atcctgcgcc
      841 atttgtggtc gatgatgaat gggaagacga tatcggtctg gtgcataacc aacgtattgt
      901 tcagtcaggt cagagctggc agcaagggcc tatcgttttg aactggttgg atatacaaga
      961 ttcttttgat gcttctggtt ttaacctgat tattcatgaa gtcgctcata agctggacac
     1021 ccgtaacggc gatcgtgcca gcggagttcc ctttattccg ttgcgtgagg ttgctggctg
     1081 ggaacacgat cttcatgctg caatgaacaa cattcaggaa gaaatcgaat tggttggcga
     1141 gaatgcggcg agcattgatg cttatgctgc cagtgatcct gctgaatgtt ttgccgtact
     1201 ttctgaatat ttctttagcg ccccagaact ttttgctcct cgtttccctt cattgtggca
     1261 acgtttctgc caattttatc aacaagatcc tttgcagaga ctgcatcacg ctaatgatac
     1321 agactcgttt tcggcgacga atgttcatta attaacaact ttgcagatta attaaccaat
     1381 tgaaatgact tatgaaattt agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc
     1441 gttcacacga ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca
     1501 ctggttcgag tccagtcaga ggagccaaat tctaaaaatt cgctttttta gcgcaatgtc
     1561 actgacctta gttgaacatt gttttttaac ggatagcggg tttttaacat cttatgcgcc
     1621 ctcgaccttt atggttgagg gcgttttgct atgaatgcca tcaccatttt cccctcgatt
     1681 ataaaacttg agttattcag tagtctcccc tcttgcaact cacacccaaa actgcctaac
     1741 gaaaagttat taattttcaa tcatattgct atcaggattt acattttttc gctgtgctag
     1801 aaagggcgca tttatgttag ctcgttcagg gaaggtaagc atggctacga agaagagaag
     1861 tggagaagaa ataaatgacc gacaaatctt atgcgggatg ggaattaaac tacgccgctt
     1921 aactgcgggt atctgcctgg taactcaact tgcgttccct atggctgcgg cagcacaagg
     1981 tgtggtaaac gctgcaaccc aacaacccgt tcctgcacaa attgccattg caaatgccaa
     2041 tacggtgccc tacacccttg gagcgctgga atcggcccaa agcgttgccg aacgtttcgg
     2101 tatttcggtg gctgagttac gcaaactcaa ccagtttcgt acgtttgctc gaggttttga
     2161 taatgtccgc cagggtgatg aactggatgt cccggcacaa gttagtgaaa aaaaattaac
     2221 cccgccgccg ggtaatagca gtgacaacct cgagcaacag atagccagta cttcacagca
     2281 aatcgggtct ctgctcgccg aagatatgaa cagcgagcaa gcggaaaata tggcgcgtgg
     2341 atgggcctct tctcaggctt caggcgcaat gacagactgg ttaagccgct tcggtaccgc
     2401 aagaatcacg ctgggcgtgg atgaagattt tagcctgaag aactcccagt tcgattttct
     2461 ccatccgtgg tatgaaacgc ctgataatct ctttttcagt cagcatactc tccatcgtac
     2521 tgacgagcgt acgcagatta acaacggctt gggttggcgt catttcactc ccacatggat
     2581 gtcgggcatc aacttctttt tcgaccacga tcttagccgt taccactccc gcgccggcat
     2641 tggcgcggag tactggcgcg actatctaaa attaagcagt aacggctatt tgcgactgac
     2701 aaactggcgc agcgcacctg aactggacaa cgattatgaa gcacgcccgg ccaatggctg
     2761 ggatgtacgc gcagaaggct ggttacccgc ctggccgcac cttggcggta aactggtcta
     2821 tgaacagtat tatggcgatg aagtggccct gttcgataaa gacgatcggc aaagtaatcc
     2881 tcatgccata accgctggac ttaactatac ccccttcccg ctgatgacct ttagcgcgga
     2941 gcaacgtcag ggtaaacagg gcgaaaatga cacccgtttt gccgtcgatt ttacctggca
     3001 acctggcagc gcaatgcaga aacagcttga cccgaatgaa gtcgctgcac ggcgtagcct
     3061 tgcaggcagc cgttatgatc tggtggatcg caacaacaac atcgttctgg aatatcgcaa
     3121 aaaagaactg gtccgcctga ccctgacaga ccccgtgaca gggaagtcag gagaagtgaa
     3181 atcactggtt tcgtcgctac aaaccaaata tgccctgaaa ggctataacg tcgaagccac
     3241 cgctctggaa gctgccggtg gtaaagtggt tacaacgggt aaagatattc tggttaccct
     3301 gccggcgtac cggttcacca gtacgccaga aaccgataac acctggccga ttgaagtcac
     3361 cgctgaagat gtcaaaggca atttttcgaa tcgtgaacag agcatggtag tcgttcaggc
     3421 acctacgcta agccagaaag attcctcggt atcgttaagt acccagacgt tgagcgcgga
     3481 ttcccattca accgccacac tgacttttat tgcgcatgat gcagcaggta atcctgttat
     3541 cgggctggtg ctctcgacgc gtcacgaagg tgttcaggac atcacccttt ctgactggaa
     3601 agataatggt gacggaagct atacccagat cctgaccaca ggagcgatgt ctggcacgct
     3661 gacgctgatg ccacagctga acggtgtgga tgcggctaaa gcccccgccg tggtgaatat
     3721 catttctgtt tcgtcatccc ggactcactc gtcaattaag attgataagg accgttatct
     3781 ctccgggaat cctatcgagg tgacggtaga actgagagat gaaaatgaca aacctgttaa
     3841 ggagcaaaaa cagcaactga ataccgcagt cagcatcgac aacgtgaaac ctggtgtcac
     3901 tacagactgg aaagaaaccg cagatggcgt ctataaggca acctataccg cctataccaa
     3961 aggcagtggg cttactgcga agctgttaat gcaaaactgg aatgaagatt tgcataccgc
     4021 tggatttatc atcgacgcca acccgcagtc agcgaaaatt gcgacattat ctgccagcaa
     4081 taatggtgtg ctcgccgatg agaatgcagc aaacaccgtc tcggtcaatg tcgctgatga
     4141 aggaagcaac ccaatcaatg atcataccgt cacgtttgcg gtattaagcg gatcggcaac
     4201 ttcctttaac aatcaaaaca ccgcaaaaac ggatgttaat ggtctggcga cttttgatct
     4261 gaaaagtagt aagcaggaag acaacacggt tgaagtcacc cttgaaaatg gcgtgaaaca
     4321 aacgttaatc gtcagttttg tcggcgactc gagtaccgcg caggttgatc tgcagaagtc
     4381 gaaaaatgaa gtggtcgctg acggcaatga cagtgccaca atgaccgcga cagtccggga
     4441 tgcaaaaggc aacctgctca atgacgtcaa ggtcaccttc aatgttaatt cagctgcagc
     4501 gaaactgagc caaaccgaag tgaatagcca cgacgggatc gccacagcta cgctgaccag
     4561 tttgaaaaat ggtgattata cggttacggc ctctgtgagc tctggttctc aggctaatca
     4621 acaggtgatt tttatcggtg atcaaagtac tgcagccctg accctcagtg tgcccccagg
     4681 cgagatcacc gtcaccgaca cagcgcctca acagctgact gcaaccttgc aggataaaaa
     4741 tggcaaccca ctaaaagata aagaaatcac tttctctgtg ccaaacgacg tcgcaagtcg
     4801 gttctcgatt agcaacggcg gaaaaggcat gacggatagt aacggggttg caatcgcctc
     4861 cctgaccggc acgttagcgg gcacgcatat gatcacggct cgtctggcta acagcaatgt
     4921 cagcgataca cagccaatga cgtttgtggc ggataaagac agagcggttg tcgttctgca
     4981 aacatcgaaa gcggaaatca ttgggaatgg cgtggatgag acaactctga cagcaacagt
     5041 gaaagatcct tttgataacg tggttaaaaa tctgtcagta gtcttccgca cctcccccgc
     5101 agacacgcaa ctgagtctga acgcgcgtaa tactaatgag aacggtattg ctgaagttac
     5161 ccttaagggc acggttttgg gggtttacac agccgaagcc acactgccta acggcaacaa
     5221 cgatacgaag atagtcaata ttgcgcccga tgccagcaac gcgctggtca cgctgaacat
     5281 cccggctcaa caggtggtga cgaataacag cgacagcgtg cagctgacgg cgacggtgaa
     5341 agatccgtcg aatcatccgc tggcggggat aacggtaaac ttcaccatgc cacaggacgt
     5401 cgcggcaaac tttacccttg aaaataacgg tattgccatc actcaggcca atggcgaagc
     5461 gcatgtcacg ctcaaaggta aaaaagcggg cacacatacg gtaaccgcaa cgctgggtaa
     5521 caataatgcc agcgatgcgc aaccagtcac cttcgtggcg gataaggaca gcgcagttgt
     5581 cgttttgcaa acatcgaaag cggaaatcat tgggaatggc gtggatgaga cgactctgac
     5641 ggcaacagtg aaagatcctt ttgataacgc agtaaaagat ctacaggtca ccttcagtac
     5701 caacccggca gatactcaac ttagtcagag caaaagcaat actaacgaca gtggtgtggc
     5761 tgaagttacc cttaagggca cggttttggg tgttcataca gtcgaagcca cactgcttaa
     5821 cggcaatggc tatacgacga cagtcaatat tgcgcccgat gccagcaacg cgcaggtcac
     5881 cctgaacatc cctgctcaac aggtggtgac gaataacagt gacagcgtgc agcttacagc
     5941 gatggtgaaa gatccgtcga atcatccggt ggcggggata acggtaaact tcaccatgcc
     6001 acaggacgtt gcggcaaact ttacccttga aaataacggt attgccatca ctcaggccaa
     6061 tggggaagcg catgtcacgc tgaaaggtaa aaaagcgggc acgcatacgg ttaccgcaac
     6121 gctgggtaat aacaatacca gtgattcgca gccggtaaca tttgtggcgg acaaaacctc
     6181 ggctcaggtt gtcctgcaga tgtcaaaaga tgagatcaca ggtaatggcg tcgataacgc
     6241 aacgctaact gcaacggtta aagatcagtt cgacaatgag gtgaataatc ttccggtaac
     6301 attcagctca gcctcttcag gactcaccct gaccccggga gtaagtaata ccaatgagtc
     6361 tggcatcgcg caggccactc tcgcaggcgt tgcctttggt gagcagacgg tcactgcatc
     6421 actggctaat aatggtgcca gcgacaacaa aactgtgcat tttattggcg acacagcggc
     6481 ggcaaaaatt atcgagttga cgcctgtccc agacagcata atcgccggta ccccgcagaa
     6541 cagctccggc agcgtcatca ccgccacagt cgttgataat aatggctttc cggtgaaagg
     6601 tgtgactgtg aacttcacca gcaacgcagc gacagccgaa atgacgaacg gcggtcaagc
     6661 cgtgacgaac gaacagggta aggctaccgt cacttatacc aatacccgct cctcgataga
     6721 atcaggagcg agaccggata ccgttgaggc cagtctggaa aatggtagct ccacgcttag
     6781 cacatcaatt aatgtcaacg ctgatgcgtc tacggcacat ctcaccttgc tacaggcact
     6841 ttttgataca gtctccgcag gcgacactac caatctgtat attgaggtga aggataatta
     6901 cggcaacgga gtaccccagc aggaggtaac cctcagcgtt tcgccgagtg aaggcgtgac
     6961 cctcagtaat aacggtataa atactaccaa ccacgacggc tatttatacg caagctttac
     7021 cgctacaaaa gccggggttt atcaagtgac ggcaaccctc gacaatggcg attcgatgca
     7081 acaaacagtg acctatgtgc cgaacgtcgc gaatgccgaa atcacgctgg cagcctcgaa
     7141 ggatccggtg attgccgaca ataacgatct cacgacacta acagcaacag tcgctgatac
     7201 agagggcaat gcgatagcca acactgaagt aacatttact ctgccggaag atgtgaaggc
     7261 gaacttcacg ctgagcgatg gcggtaaagc gattactgat gctgaaggca aagcgaaagt
     7321 cacgctgaaa ggtacaaaag caggcgctca tactgttaca gcatcgataa ctggcggtaa
     7381 gagtgagccg ttggtggtga actttactgc ggatacgctc actgcgcagg ttaatcttaa
     7441 cgttaccgag gacaatttta tcgctaataa cgtcgggatg accaggctgc aggcaacagt
     7501 gactgatgga aacggcaacc cgttagccaa tgaggcggtg acattcacgc taccggcaga
     7561 tgtgagcgca agctttactc tcggacaagg cggttccgcc attactgata tcaacggcaa
     7621 ggctgaagtt acactgagcg gtacaaaatc cggcacctat cccgtgacag ttagcgtgaa
     7681 caattatggt gtcagtgata cgaaacaggt gactttgatt gccgatgcag gtaccgcaaa
     7741 actggcttcc ttaacctctg tatattcatt cgtcgtcagc acgaccgagg gcgcaaccat
     7801 gacggcaagc gtcactgacg ctaacggcaa cccggtagaa ggtataaaag ttaatttccg
     7861 cggaacttcc gtcacgctaa gcagcaccag cgttgaaacg gatgatcggg gtttcgctga
     7921 aattcttgtg acaagcaccg aggtcggact gaaaacagtt tcagcctctc tggcagataa
     7981 acctactgaa gtcatctcgc gattactgaa tgcaaaagca gatattaatt ctgcaacgat
     8041 taccagtctg gagatacctg aaggtcaggt catggtcgca caagacgtag cagttaaagc
     8101 tcacgtcaac gaccagtttg gcaatccgat tcttaatgaa tctgtaacat tcagtgcaga
     8161 accaccagag cacatgacca tcagccaaaa tattgtctct actgatacgc atggtatagc
     8221 cgaggtcact atgacgcccg aaagaaacgg ttcgtatatg gtgaaagcat ccctggcgaa
     8281 tggatcctct tatgagaagg atctggtggt aatcgatcaa aaactgacac tctcggcgtc
     8341 cagcccgctt atcggtgtca attccccaac aggtgcaact ctgacggcaa cgctaacttc
     8401 tgcaaatggc actccagtgg agggtcaggt catcaacttt agcgtaacgc cagaaggtgc
     8461 gacgttaagt ggcggaaaag tgagaaccaa ctcttcaggt caggctccag tcgttctgac
     8521 cagcaataaa gtcggtacat atacggtgac tgcatcgttc cataacggtg taacaataca
     8581 gacacagaca atcgtgaaag tcactggcaa ctcaagtacc gcccatgttg ctagctttat
     8641 cgctgatcca tcgactatcg ccgccaccaa cagtgattta agtaccttaa aggcaacggt
     8701 tgaggatggc agtggtaacc tgatcgaagg tctcactgtg tacttcgcct taaaaagcgg
     8761 ctctgccaca ttaacgtcat taacagcggt gaccgatcaa aacggaatcg cgacaacaag
     8821 cgtgagagga gcgataacgg ggagcgtcac ggtaagcgca gtcacgaccg ctggtggaat
     8881 gcaaacagta gatataacgc tggtggcagg cccggcagac gcctcaaagt cagtccttaa
     8941 gaacaatcgg tcatcattga aaggagactt taccgatagt gctgagctac atcttgttct
     9001 gcacgatata tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg tgcagtcagg
     9061 taccaacgtg ccctatgtgc aagttagtgc aattgactac agtaaaaatt tctcaggcga
     9121 gtacaaagcc actgttacag gcggcggaga gggtatcgca acgctgatcc ctgtattgaa
     9181 tggtgttcat caagctggtc tgagtaccac aatacaattc actcgtgcag aagacaaaat
     9241 aatgagcggt acagtgtcag tcaatggtac tgacctaccg acaactacat tcccttcgca
     9301 gggattcacc ggggcgtatt atcagttgaa taatgacaac tttgcccctg gaaaaacggc
     9361 ggctgattat gagttttcaa gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt
     9421 gacatttaaa aatgtcggca gcaattggga aaggattacg gcgacgccaa aatcaggagg
     9481 ccctagctat gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg gcgaggcttt
     9541 catgatatac agccttgctg aaaatttttg cagcagcaat ggctacacgc tccccagagc
     9601 aaactattta aaccacagtc gttcccgagg catcgggtca ctgtacagtg aatggggaga
     9661 tatggggcat tacacgactg aagctggttt tcaatcaaat atgtattggt catctagtcc
     9721 cgcaaactca aacgaacaat acgtagtttc cctggcaaca ggtgatcaaa gcgtatttga
     9781 aaagcttggg tttgcttatg cgacatgtta taaaaacctc tgattttccg ttataatttc
     9841 ttaaagagtg ctccattcgg ggcactcttt tttacattcc ctctatagag cttgctcttc
     9901 tctcaccgaa aatgaataaa taaaaatgcg ataccgccag attcgcgttc ggtgtgacta
     9961 tgatcgtatc cggtgagtac agctttgatc caaaggctgg ttttctgtga tgattgacca
    10021 gcctcagagt attgatgaca ccaccgttca taacagatcg gcattactat gcataagtac
    10081 tgtctcatat tctcgccaaa acacttttta atgcctttgc gaattcatcc agacttatat
    10141 tgtcaatttt ttgcccaacg acgactgact ccgaaacatc ggccattgcg ggccatcgcg
    10201 ttttgtaatc agcataaatt gcaacaacgg gagtattata ggcacttgcc atatgtaata
    10261 ttgaggtgtc aggagttatt gcgatataag catcttttat gattgctgct gaacgtttaa
    10321 tcgagggtga taaggataaa cggtagacat tattataacg atccactaat gccctggctt
    10381 taccttcccc ttttggtcca tgaacgatca ctattggtat atctgtttca ctttgaattt
    10441 ttgctattag attttctgca atcaataatg aaaatgtacg ttcctgcgag ctaccttcta
    10501 aattaagcgc aatgtaagat cctaacgatc gcatttcctc gcgcacctca tcgagtacat
    10561 cctcacttag tggcaattca tatattggcc tgactgctgg aaaacccgcc tcacgcatca
    10621 taaatgccca tgtcatgggt acgggagccc ggagattctg atccatccgg gacgcgttct
    10681 tacacgcagg ggaatagcac ttcatggtta aaccaacaac ctgaaaattc gtttttgctt
    10741 tcaactgact aataaataac atcgttttca ggttcttttt acgcatcgcc tctatacaaa
    10801 gatccggcgt accgtattgc tgtgttatgt tcttcgctaa atcttttatt tcttttaatg
    10861 ttgcgtgatc ctgca
//
LOCUS       DAZUCE010000175        10862 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZUCE010000175 DAZUCE010000000
VERSION     DAZUCE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.175"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            80..511
                     /gene="osmC"
                     /locus_tag="ACHY03_003772"
     CDS             80..511
                     /gene="osmC"
                     /locus_tag="ACHY03_003772"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFS3906952.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(567..1493)
                     /locus_tag="ACHY03_003773"
     CDS             complement(567..1493)
                     /locus_tag="ACHY03_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFS3906953.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTFAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(1486..2472)
                     /locus_tag="ACHY03_003774"
     CDS             complement(1486..2472)
                     /locus_tag="ACHY03_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3906954.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNARETQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(2469..3365)
                     /gene="ddpC"
                     /locus_tag="ACHY03_003775"
     CDS             complement(2469..3365)
                     /gene="ddpC"
                     /locus_tag="ACHY03_003775"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFS3906955.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(3362..4384)
                     /locus_tag="ACHY03_003776"
     CDS             complement(3362..4384)
                     /locus_tag="ACHY03_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3906956.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(4386..5936)
                     /locus_tag="ACHY03_003777"
     CDS             complement(4386..5936)
                     /locus_tag="ACHY03_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFS3906957.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQAELRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVKSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(5950..6531)
                     /gene="ddpX"
                     /locus_tag="ACHY03_003778"
     CDS             complement(5950..6531)
                     /gene="ddpX"
                     /locus_tag="ACHY03_003778"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFS3906958.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(6789..9188)
                     /gene="dosP"
                     /locus_tag="ACHY03_003779"
                     /gene_synonym="pdeO"
     CDS             complement(6789..9188)
                     /gene="dosP"
                     /locus_tag="ACHY03_003779"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFS3906959.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(9213..10595)
                     /gene="dosC"
                     /locus_tag="ACHY03_003780"
     CDS             complement(9213..10595)
                     /gene="dosC"
                     /locus_tag="ACHY03_003780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFS3906960.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
BASE COUNT         2697 a         2812 c         2692 g         2661 t
ORIGIN      
        1 attcggaata tcctgcttat cctcgtgctg tttctcacgt agtctataat ttccttttta
       61 agcccacagg agagcaacaa tgacaatcca taagaaaggt caggcacact gggaaggcga
      121 tatcaaacgc gggaagggaa cagtatccac cgagagtggc gtgctgaacc aacagccgta
      181 tggatttaac acgcgttttg aaggcgaaaa aggaaccaac cctgaagaac tgattggcgc
      241 agcgcatgcc gcatgtttct caatggcgct ttcattaatg ctgggggaag cgggattcac
      301 gccaacatcg attgatacca ccgccgatgt gtcgctggat aaagtggatg ccggttttgc
      361 gattacgaaa atcgcactga agagtgaagt tgcggtgccg ggtattgatg cctctacctt
      421 tgacggcatc atccagaaag caaaagcagg atgcccggtc tctcaggtac tgaaagcgga
      481 aattacgctg gattaccagt tgaaatcgta aagcgttgcc ggatgacgcg tcaggcgcgt
      541 gaatgcctga tgcgttgtta gcatctctac agcgcccgcc agcaccggag ctcacgtccg
      601 tcctccctta ttgctaatga ttgccggact tcgcatccgc tggttgcaag ggggcaacgt
      661 tcgtagaaaa aacagccttg cggcaacgtg cggtttcccg gcagatccgt tttacgtaat
      721 gcccattctt cctccagcgg tttatcaatg gcggggaggg aatccagcaa taatcgggtg
      781 tatggatgtg ccggagccgt tagcacctgt tgcgcttccc ctaattccac aatctgcccg
      841 agatacatca ccgctacccg atcgctcata tgacgaatca ccgagacatt gtgtgagatc
      901 agcacatagg tcagcccgcg atttacctgt agcgttacca gtaaattgag gatctgcgcc
      961 tgcacggaga tatccagcgc cgaggttggc tcatcaagca caatcacgtc aggctgcgaa
     1021 gagagtgctc tggcaatggc gatgcgttgc cgctgcccgc cggagaacgc atgaggcagg
     1081 cggtcgagat actccggacg aatacccacc tgcacagcca gctcctctgc caacgctcgc
     1141 cgctgttgtt cactactacg cttagctatc cagagcggtt ctgtgatgat gcgccacacc
     1201 ggtaagcgcg gattaagcga agagagcggg tcctgaaaca ccatctgcat aatgcgttgt
     1261 gatcctgaac ggatgtattg cccgtggctc ggttgcagca tacccattaa aagctgtgcg
     1321 aaggtgcttt tgccgcagcc tgactcaccg acgatcccta aggtttcacc acggcggatc
     1381 tgtaaatcaa taccattaat cgcatgaaca tgttccgtag ttttaccaag ccagttttta
     1441 cgggccggga aattgatatg gacgtcgcgt aacgttaata acgtgtcaga cacttatgac
     1501 ctcctgctgc ggataccaac aggcgcagcg ctggttgttg tcaccacacg ctgtcagcgc
     1561 cgggacgttt tcacactgtg cacctgccgc atagcaacga tcgcgaaaag cgcagccgtc
     1621 aggcaaatgg gtgagatttg gtaccgtccc tggaatggcg ggtaatggct ggcgcggtat
     1681 tccatgttct ggtgcgcatt gcagcaaacc aatggtatac ggatgccggg gatgatggat
     1741 aacgtctgcc gtcacgccgc tttcaattac gcttccggca tacatcacgt aaacgctatc
     1801 gcacagttgc gacaccacgg ccatatcatg gctgatgaac agtaccgcag ttccactggc
     1861 gcgggcttta tgtttaagca aacgcagtac ctgcaactgt accgtgacgt ccagcgccgt
     1921 agtcggttcg tcggcaataa tcaattgcgg ctcgcaggag aatgccagcg caatcattac
     1981 ccgctggcgc ataccacctg aaagctcaaa cggatagcgc gacataactt ccactgcatc
     2041 cgggatttgc atctcttcca gcagggcaat cgctttagct ctggcttccc gacgacttat
     2101 tggttgatga tggcggatca cgtccatcat ctgaagacct attcgacgtg tcggattaag
     2161 ggcggtcatc ggttcctgaa agatcatcgc cactcgtgcg ccgcgccact gacgaagctg
     2221 tgtttcccgc gcgttaagaa catcttctcc cagcagggaa atctgccccc gatgtacgca
     2281 ataactgccc gtcggtagca gacgcataat cagcattgcg gtgactgatt tacctgagcc
     2341 ggattctccc accagaccga caatttcacc gcggttaatc tgcaaggaca cattgttgag
     2401 cgcgtgaaca tcaccgttaa aaccggggaa actcaaatgc agttgttgaa tgtccagaac
     2461 gggttgggtc atgactgctt tcctcctgct ttcgggtcaa gcagatcgcg aataccatca
     2521 ccaaagagat taaacccgac ggcggtaagc aaaatcgctg cccccggaaa tgcgcaatac
     2581 caccactgat cgagtacata gttgcgacca tttgctacca tcgcgcccca ttcagcactc
     2641 ggttgttgag cacccaggcc aataaatccc aacgtggcgg ccattaaaat cgcgctaccg
     2701 atatccagcg atgcctgcac gatcagcggc ggtagggagt tacgtaaaat atgccagttg
     2761 atcagatgcc aacgagacgc gccaaaggtt ttcgccgcct gaacataggt atactggcgt
     2821 acaactaatg cttgcccccg cgccaggcgc acataaaagg gaattcgcac aatagcaata
     2881 gccagcatgg cgttaaacaa actcggcccg agagcggcag ccagtgccat tgtcagtacc
     2941 aacgaaggaa tcgacagcat aatgtccatg atgcgcatga tgatggcgtc tgcgcgtccg
     3001 ccaagcacac cggatagaca tccgagtagc gaaccaatca tacccgcaat ggcaaccacg
     3061 actaatccgg cgagaattga ttgctgactg ccgaccagta cgcggctgaa cagatcgcgt
     3121 cccacttcat cggtgccaaa ccagtgcgcc gcagaaggcg gcaaaaggcg ggcggttaaa
     3181 tcaatggcgt tgggatcatg cgtcgccagc cacggtgaaa aaatcatcat cagtagcatt
     3241 aatacaatga tgactgcgcc ggtcacggtg agcggactgc ctttcagcat ccataccagt
     3301 tttgcaccgt taaatcgcgt ttgtttttgt gggcgtacgg cggacgtttc ctcgcttagc
     3361 atcattcacc acctccacgt ccgatacgcg gatcaatcca caaatagagc aaatccacta
     3421 ccaggttcac cagcacataa gcaaacgaaa ccacgacggc aaatcccatc actgccggga
     3481 agtcgagcgc ctgtattgat gtcactaccc atgcccccat tccgggccag gcaaaaacgg
     3541 tttcggtgag cactgcgcca tacaacaaat cgcccagcgc cagccccaat acggtaatcg
     3601 atgggatcaa cgcattgggt agcgcataac atagaacgat ataccagccg ggcaagccgc
     3661 tggcccgggc ggtacgaatg tagtcttcac tcaattgttc cagcatcgct gagcggatct
     3721 ggcgggcgac aatccccagg tgaacaaacg ccagcgttaa tgccggtaag atgagatgtt
     3781 gcaacgcatt gaagaagact tcaccgttgc cttcaagcaa cgcatcgagc agataaaagc
     3841 cggtgacgtg cgttggtgga tccagccagt catcaagcct tccgccgccg ggaagtattt
     3901 gcagatgacc ataaaacagc acaatgacgc ccagcccaag ccagaacgca ggtgtggaga
     3961 ttccggtaat ggccattatt cgcaccagat gatccagcca gcgatttcgc cagactgcag
     4021 agagtatgcc cagcgggatg ccaatcaaga gtgccagcag cagggcacaa aaagccagct
     4081 ccagcgtcgc cgggaaaaat atacgcaact cttccagcac cggacgcccg gtacgaatgg
     4141 atgttcccag gtcaccatga aacaggtcgc tgacgtagcg gtaaaactgt acatacagtg
     4201 gctggtccag tcccagttgc tggcgaatat tttccacgat agcatcgctg gcgcggtcac
     4261 ccgccaataa ccgcgccgga tcgccgggga tcagatgcga gataataaac gtaatcacac
     4321 agacccccgc catcacgagc accagccccc aacagcgttg gcgtaaaata ctccagaagg
     4381 tcacgttatt tactcatggt attgatattg aagacctgtt ccagcatggg attgaacaca
     4441 aagcctttca cctctttgtt catcgccagt tggtagtttt tctggaacag gtacacataa
     4501 gcagcgtcat caatgacgat tttctgtgcc tgctggtagt ctcgggtacg ctgcgtctgg
     4561 tcggtggtcg caagcgcatt gcgcagtaac ttatcgacct cactgttttc atagaacgag
     4621 cggttacccg gcagaccttt tttgtctgac tcaaaccagt aattcataaa catatacggg
     4681 tcggcaaaat ccggactcca gttgccaatc gcaatgtcgt aatcaccttt acccactctg
     4741 tcgcgcatgg tggcgttcgc cagcttttcc agcttcacat tgatgcccag cttgttgaga
     4801 ctggattgtg tcgccagagc aataggctcc cagttcggat cgttatcgga gtagagaaac
     4861 gtcaggctgg tgggtttgct cttcacttta tcccattcgg ctttggcttt cgtttcgtca
     4921 tggttgtatt gcattgccgt cgcatcgtag ccccacatgc cttccggaat cgggccgcgc
     4981 atctgttttc cgttaccact cagaatgccg ttaaccatgc cctgataatc ggtagaccag
     5041 gaaatggccc gccgcagctc cgcctgatta agtggcgctt tgctgttatt cagatacaga
     5101 taggtgacgc gcagtgacgg atactctgcc acattgactt tgttttcctg cttcagggcg
     5161 ttgagttgat ccaccggcag cgcatcggca atgtcaatgt cgccacggga gagctgcagg
     5221 cgacgggagg cactttcacc gataattttt accgatactc gcttaaagtt cggtttattg
     5281 ccggggtaat gcggatttgg caccagaact aattgctgac ctttttgcca gcttttcagc
     5341 ataaatggtc cggaaccggc ggtattttgc gcgaggaagc cgcgagcatc atccgccgca
     5401 tgttccttta acaccgccgg attgataatg gatgcaccgt cattcgccag cgtatagagg
     5461 aacggtgcga atggctggct aagggtaaac ttcaccgtat gttcgtcggg agtatcaatc
     5521 tttaaatctt tgggaaatgc ttctgctggc ccctggccga tttttagtaa ccgctcaaaa
     5581 gagagtttta ctgcttcggc agtgacaggt gtgccatcgg caaatttagc attgtccttc
     5641 agggtgaacg tccactcttt ttgatcgtca gacgctttcc agctacttgc cagatcgcct
     5701 tcaacgtcgg ttgagccttt atcaccgtcc gttttgtact gaaccagccg ctgataagac
     5761 gggtaggtca ctgtccagtc gttattatct attgttaccg ccgggtcgag ggtttgtgga
     5821 tcggcggcct taccaatcac cagcatatct ttcggtacgg cggcgtgcgc aaccgggaaa
     5881 gttgtggcaa ggacgagcgc gagcaatgtg ggacgaaacg atatcgatct cttcatgaca
     5941 gtttcctgat taactgacgt gctgtgtgcc aggggatata aaacaggtga attgatcagc
     6001 gagcagaggg taactcgctg cctggggtaa ttcgaagtgc caccattcgc ttgagatacc
     6061 gacaaaaccg ccaccagtca ttatcgcatt cagcaacagc cgattgcgct gagcggcggg
     6121 cgggacggaa gggtgatagg catgggagcg ctcgtgcatt tcatcgaacc ctgcgcccat
     6181 atccaggatg ttcccgtgct catcacgaag cgtcaggtcg atcgccgtgc cacggctgtg
     6241 attagaaccg accgtcacat caacaacata ttgcgggtct gggcaggctt gccacaacat
     6301 cgcctgtgct tgttgtgggc gatacgcatc gtaaatcacc agttgtaagc ctgatagctg
     6361 ggcgatactg atacttttcg ccagcgcggt aatcgcatcc ttgtgtaaca gacaacgcgc
     6421 ttgctggtaa atagctttac ctgtgatgtt atcagcgcag gcgtatttca attcgatctc
     6481 cagatcaggg aagattacgg ctaaatcaac cagttcggtg gtatccgaca taaacggttc
     6541 ctgtataaga caaaaattgc tgcgctttcc gcttatgcag atctcatgcc atgccgggat
     6601 aagcgccaga atgctggctt aaagttatat tttttaactt ttgatcaaca tttgtgcagc
     6661 gtagtgcagt tttggtgcaa gaggggaagt taaggaagga atctcccgga atcgtagctg
     6721 aaatcacagt atttaagtga cagtgtcacg ttaaatgaaa acccgcgagt gcgggcgaga
     6781 ggaatttgtc agattttcag cggtaacacg ctgctcatcc agcctggaat ttcttcggcg
     6841 ggtagggggc gggaaaagaa atatccctga ataacgcgac agtggatctt gcgtagcatc
     6901 tcaaattgct ctttggtttc gacgccttcc gccacgacgg ttaaattgag gctttgccca
     6961 atgctggtaa tggcttcaag taaggcaagg atgcgttttt ccgtcagaca acgatcgaca
     7021 aaacttttgt caattttgat ttccgttacc ggaagactga ctaagcggga taatccggaa
     7081 aagcccgtgc caaaatcatc taccgataaa cccacgccca tatcacgcag gatctgaatg
     7141 cgcttaaaga tttcggtatc gtgttccatc atcatgcttt ctgtgatttc caccgtcagc
     7201 tggtggccgt caatacccca ggcttgcatt gcatcagaca cctgattagg cagttgatta
     7261 ctgcgaaagt gcagcgccga caagttcacg gataacgccg ggatatgaat attctggcta
     7321 cgccattctg ctaactgacg gcaagcttcc gcgatgaccc agcgcccaat attttcgatt
     7381 tcaccaatct cttctgcgag aggaataaac cgtgaagggg gcacatgacc atgctgggga
     7441 tcgtgccagc gagcaagggc ttcgatgccg tagagttcac ccgtttctgc gaagatttgc
     7501 ggctggtaaa ccagtttcag ttggttattg ctaatcgcct ctttcagcgc tgcgcctaaa
     7561 accaaacgct cttttaccat ttcgttcatc gccgggctga agaactgcca gccgttaccg
     7621 ccattcttgc gaatataatc cattgcattg tgagcagtgg agagcaggta atcgcggttt
     7681 ttacccacgt cgtagctgat gccaatactc aaggtaagcg ggaagggttt atcgtcaatc
     7741 attatcggtt tgctgaccac attccgtagc tcatcggcga tttgggtaat gttactgacg
     7801 tcgttttcca ggctcacgag gacaaactgc gtaccttcga tacgacagag atactgatcc
     7861 ggtttgagtt tttcacgaaa gcgattgacc acttccagca atgcctgatc ggcccacgca
     7921 tagccaaggc tatcaatcac atcctgaata tggtcaacac cgatgagata caccaccgga
     7981 gagacggctt tgtcgaccag gtcatcgagg taattgtgca ggttattgcg atttggcaga
     8041 ccggtcatcg gatcaaattg gatgagttgt tcaatatgct gacggctttt ttcctgttcc
     8101 agcgccagcg cggccatatg ctggctgata tctgccacgc gttcgataaa ggcgctggtt
     8161 tctgctcctg acgaggtttt aatttgcagg atccccgcag gcgcgccatc acgctgacga
     8221 atggtcgctg accagctttg cgcattttga acttctgcac cgtgcgaaga tgacgcccag
     8281 tgtatcggca tcccgttgcg cagtgcgaac agcgaaacat gcgattcgtt gagaacagat
     8341 tcgatgttac gacaaatgat ttcccccatt tcatgaaatg gcgggctgct gcacatggcg
     8401 gcgagaatat tgccttcaag ctggcggatc tgccgttctt cggtgatatc cgagaaagtc
     8461 attaccaggt tctgcagatg cgcgagcacg tcataaaccg ggcttataga ggctttaatc
     8521 cagatttttt caccggtgcg cgtcagcagc agaaattcgt cctgatcgcg ggcggttttc
     8581 catagcaact gttgtaaacg aatgcggtta tcggcaggga attcaggaat gttcaggagt
     8641 gtatcgggct gcataccgct ggcttcgcta atgcagtaac caaacatttc ggtaaatgcg
     8701 cgattgcact gcacaatatg gcgttccgga tcgagaacaa tcaccggtcg gtcgagatgg
     8761 tcaacggcaa taatcaattg tcgggtctgt tctttttgcg ccatttctac gctggcatcc
     8821 cgtaccagcg ccaggtaata aactttcccc tcggcgctca ctttcgatag cgcaaaacgg
     8881 gtccagattt tactgccgtc ttttttctcc agctgcagct cccgactcat cccctcaaca
     8941 cgcgctttac cgccttcacg gttgtgacga atgtattcag gatgcgcagg acgcaaatcc
     9001 cgcggaatca gcatatcaat gttattgcca atgacttctt cacgtttgta tccccagagc
     9061 ttctctgcgg cggggttgaa aaacatcact tcatcatttt cgttaattaa caccgcaccc
     9121 atcatatttt gctcaagggc ggggaaaaaa atgccatcgg cggcattatc cgcatcggtt
     9181 agcttcatga ttacctctgc atcctggcgc atctaaagac tggctttcca gagttcaaca
     9241 cggtttctac ctcgtctttt ggcgatatac agagcttcat cggctatttg aatgaggcgc
     9301 tcatagtcag gatgaccatt aaacatggcg gcaccgatgg aaagtgagag ggcaatatcc
     9361 tcgccgtttg cggctttcag tttggttttc tccacccgac tgcgaatacg ttctgcggta
     9421 cgtaacgttt cgttttcaga agcttcagtc aaaacaatga taaattcatc gcccccgtag
     9481 cggaaaacat aatcactact gcggacgttg tcataaaagg cctgagagac tttacgcaga
     9541 atttcatcac cagtgttatg gccccacgta tcgttgatct ctttgaattt atcaacgtca
     9601 ataatcagca ctgacagcgg tgtaccggtc cggttggcat gggcaatttc gcgtttgaag
     9661 atagtcggca ggaaacggcg gttaagtaat ttcgtcagta catccatacc gacttcgtgg
     9721 cgcgatactt cttcaaacaa ttcacgcagc aaggtaataa tttgcgatac agtatttctt
     9781 atctgtaata aaaatttcac ccgcagactt ctgttattca aattcctggt gttacgcatg
     9841 gtttgattga aaataccgtc gaaatcctga atcagacgag agatatggcc cacttcagca
     9901 atgccgctga aataatgtcg acctttatgg ttaaaccaca ggccaaaatc agcctggctt
     9961 aaaggcaaac tactgcctaa atcagaatcc agcaggattt tatagatgat atctatttcc
    10021 catgaaagta ttgaggctat ttgccgttct ttttcttctt cggcgttttc cagtaacgag
    10081 aagatacgat agttttcatc ttcttttgag gcactactgt cactaaaggt aaacgcgcgg
    10141 gtcatcactt ccatagcgat atcaatactg ttaatcgaga aatggtagac ctgaagtttt
    10201 tctgcggcag aataatccga agagaagatt accggataga ggatcttttt cagcacccga
    10261 aaccccatct cgacaatttc taccggaatt cctatgcggg catgcacttc cgcgacggta
    10321 tgctggattt gtattagcct ttcgacatcg tcaacctggg cagaaagcac gttaataatc
    10381 cagcgttcca tcgcactttt caactgccgc tcaacttgtt cattactcaa gaattcttcg
    10441 gcatgcgggt caatgcggac aattcgataa aactcaatac tcagataatg agcatgcgca
    10501 acggcaattt ccgcggcttt agctcgaatg ggcggatctg cctgttcgac aagtccggtc
    10561 cactcatctt tcattctttt aaaatacatc tccataattc acacccttat aaggctggga
    10621 aatcagacgg aatcaaaacg aaacgcaacg tgcgagatcg actaactgca ccatattctc
    10681 ctgaaatatg aagatatact gaaaagaaat aagcgactta ggacagtttc aatctacgct
    10741 actgttcttc agaagagtat agcccatcgt aattattttt cggtgacagc gaatatcgta
    10801 tgttttttca tattcataca tttttattag gggatttatg gctgtttaac taagtgtggt
    10861 ta
//
LOCUS       DAZUCE010000176        10801 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZUCE010000176 DAZUCE010000000
VERSION     DAZUCE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.176"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            28..789
                     /gene="adiY"
                     /locus_tag="ACHY03_003781"
     CDS             28..789
                     /gene="adiY"
                     /locus_tag="ACHY03_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFS3906961.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            926..2263
                     /gene="adiC"
                     /locus_tag="ACHY03_003782"
     CDS             926..2263
                     /gene="adiC"
                     /locus_tag="ACHY03_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFS3906962.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2367..4010
                     /gene="eptA"
                     /locus_tag="ACHY03_003783"
     CDS             2367..4010
                     /gene="eptA"
                     /locus_tag="ACHY03_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFS3906963.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4007..4675
                     /gene="basR"
                     /locus_tag="ACHY03_003784"
     CDS             4007..4675
                     /gene="basR"
                     /locus_tag="ACHY03_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFS3906964.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4676..5776
                     /gene="pmrB"
                     /locus_tag="ACHY03_003785"
     CDS             4676..5776
                     /gene="pmrB"
                     /locus_tag="ACHY03_003785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFS3906965.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5752..5841)
                     /gene="pmrR"
                     /locus_tag="ACHY03_003786"
     CDS             complement(5752..5841)
                     /gene="pmrR"
                     /locus_tag="ACHY03_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFS3906966.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5953..7455)
                     /gene="proP"
                     /locus_tag="ACHY03_003787"
     CDS             complement(5953..7455)
                     /gene="proP"
                     /locus_tag="ACHY03_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFS3906967.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7719..8597)
                     /gene="rdcB"
                     /locus_tag="ACHY03_003788"
     CDS             complement(7719..8597)
                     /gene="rdcB"
                     /locus_tag="ACHY03_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFS3906968.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAEAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(8594..>10801)
                     /gene="crfC"
                     /locus_tag="ACHY03_003789"
     CDS             complement(8594..>10801)
                     /gene="crfC"
                     /locus_tag="ACHY03_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFS3906969.1"
                     /translation="ELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQRALPWSQPNI
                     AERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTLI
                     RHTPGQKEPVLHFSHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENG
                     VAFDKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGL
                     ESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAI
                     LAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLA
                     NRARHELANNDKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLF
                     AQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQ
                     IEESLQLLQLNQAQVSGEIKHEIELALTSANLFLRQQQDALKAQLAALFQDDSEPLSE
                     IRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRPG
                     FHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPDWGRGNEK
                     SPTETVDYSVLQGALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQT
                     RLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT         2701 a         2838 c         2670 g         2592 t
ORIGIN      
        1 cttttattcg cactcaggag tacatgtatg aggatttgca gcgaccaacc ttgtattgtt
       61 ttattgactg aaaaagatgt ctggataagg gtgaatggga aagaacctat tagccttaaa
      121 gctaaccata tggcgttatt aaattgtgaa aataatatta tcgacgtctc ctctcttaac
      181 aacactttgg ttgctcatat tagtcacgac atcatcaaag attacctccg gtttctgaat
      241 aaagatctct cgcaaatacc agtatggcaa cgtagcgcta cgcccattct caccctgcca
      301 tgcctgacac cagacgtctt tcgcgttgcc gcgcaacaca gcatgatgcc cgcagaaact
      361 gagtcagaaa aggaacgaac acgcgcatta ttattcacgg tgctatcccg ttttctcgac
      421 agtaaaaaat tcctttcatt aatgatgtat atgttacgta attgtgtaag tgacagcgtt
      481 tatcaaatta ttgaaagcga tattcacaaa gactggaatc ttagtatggt agccagttgt
      541 ttatgtctta gcccaagtct gttaaagaaa aagctgaaaa gcgaaaacac cagttatagc
      601 caaataatca ccacctgccg catgcgttat gccgtaaatg aattaatgat ggacggtaaa
      661 aatatctctc aggtatcaca gtcctgcggc tacaacagta cgtcgtactt tatttctgtc
      721 tttaaagact tctacggtat gacgccgctg cattatgtta gtcagcacag agaacgcact
      781 gtcgcctgat ttttaacctt aacgaagagc tatattaata acggcatcag cgataacccg
      841 gtcgataata attcaactat cgaatgcagg cgtatgatat gacgtaattt attgtcacga
      901 agctcgcctt cgcaggagtt taattatgtc ttcggatgct gatgctcaca aagtgggctt
      961 aatccccgtc accctgatgg tgtcggggaa tattatgggg tcaggtgttt ttctgttacc
     1021 tgcaaacctg gcctccactg gcgggattgc catatatgga tggttggtga cgattatcgg
     1081 tgcgctgggg ctctcgatgg tatacgccaa aatgtcgttc ctcgatccaa gtcctggtgg
     1141 ttcttacgct tacgcccgcc gctgctttgg cccgtttctc ggttatcaaa ccaacgtcct
     1201 ctactggctg gcctgctgga tcggcaatat cgccatggtg gtcattggcg taggatattt
     1261 aagttacttc ttcccgattc tgaaagatcc attggtatta accatcacct gcgtcgtggt
     1321 gctgtggatc ttcgtcctgc tgaacattgt cggtccgaaa atgatcaccc gtgtgcaggc
     1381 cgttgccacc gtgctggcgc tgatccccat cgtcgggatt gccgtatttg gctggttctg
     1441 gttccgtggt gaaacctata tggcggcatg gaacgtcagc ggcctgggca ccttcggtgc
     1501 aattcaaagt acccttaacg ttacgctgtg gtcgttcatc ggtgtggaaa gtgcctccgt
     1561 tgccgcaggt gtggtgaaaa acccgaaacg caatgtccct atcgccacca tcggtggggt
     1621 attgattgcc gccgtttgct atgtgctctc caccaccgcg attatgggga tgatccctaa
     1681 tgccgcactg cgcgtttctg cctcgccatt cggtgatgcc gcacggatgg cgttgggtga
     1741 caccgccggg gccattgttt ccttctgcgc agctgcgggt tgcttaggtt cactgggcgg
     1801 ctggacattg ctggcgggtc aaacggcgaa agccgctgcc gatgacggac tgttcccacc
     1861 gatttttgcc cgtgtaaata aagcgggtac gccagtggcg gggctgatta tcgtcggtat
     1921 tttgatgacc atcttccagc tcagcagcat ttcaccaaac gcgaccaaag aattcggtct
     1981 ggtttcttcc gtgtcggtca tctttacact ggtgccatat ctttacacct gtgcggcgtt
     2041 actgctgctc ggacacggtc actttggtaa agcacgcccg gcatatctgg cagttactac
     2101 cattgccttc ctctactgca tctgggccgt ggtggggtcc ggggcgaaag aggttatgtg
     2161 gtcatttgtc accctgatgg tcatcaccgc catgtatgcg ctgaattaca accggctgca
     2221 taaaaacccg tatcccttag atgcaccaat aagcaaagat taattcccct taatccagca
     2281 aagataaaag ccaaccttaa gaacttaagg ttggcttaat tttgctttgc gagcatatgc
     2341 gcactttgtt cgatggaaac accgtgatgt tgaagcgcct actaaaaaga ccctctttga
     2401 atttactcgc ctggctattg ttggccgctt tttatatctc tatctgcctg aatattgcct
     2461 tttttaaaca ggtgttgcag gcgctgccgc tggactcgct gcataacgta ctggttttct
     2521 tgtcgatgcc ggtcgtcgcc ttcagcgtga ttaatattgt cctgacacta agctctttct
     2581 tatggcttaa tcgaccgctg gcctgcctgt ttattctggt tggcgcggct gcacaatatt
     2641 tcataatgac ttacggcatc gtcatcgacc gctcgatgat tgccaatatt attgatacca
     2701 ctccggcaga aagttatgcg ttgatgacgc cgcaaatgtt attaacgcta ggattcagcg
     2761 gcgtgcttgc tgcgctgatt gcctgctgga ttaaaatcaa acctgccact tcgcgcctgc
     2821 gcagtgttct tttccgtgga gccaatattc tggtttctgt actgctgatt ttgctggtcg
     2881 ccgcactatt ttataaagac tacgcctcgt tgttccgcaa caacaaagag ctggtgaagt
     2941 ccttaagccc ctctaacagc attgttgcca gctggtcatg gtactcccat cagcgactgg
     3001 caaatctgcc gctggtgcga attggtgaag acgcgcaccg caacccgtta atgcagaacg
     3061 aaaaacgtaa aaatttgacc atcctgattg tcggcgaaac ctcgcgggcg gagaacttct
     3121 ccctcaacgg ctacccgcgt gaaactaacc cgcggttggc gaaagataac gtggtctatt
     3181 tccctaatac cgcatcttgc ggcacggcaa cggcagtttc agtaccgtgc atgttctcgg
     3241 atatgccgcg tgagcattac aaagaagagc tggcacagca ccaggaaggc gtgctggata
     3301 tcattcagcg agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc tgtaaaggtg
     3361 cctgcgaccg cgtgcctcac cagaatgtca ccgcgctgaa tctgcctgat cagtgcatca
     3421 acggcgaatg ctatgacgaa gtgctgttcc acgggctgga agagtacatc aataacctgc
     3481 agggtgatgg cgtgattgtc ttacacacca tcggcagcca cggtccgacc tattacaacc
     3541 gctatccgcc gcagttcagg aaatttaccc caacctgcga caccaatgag atccagacct
     3601 gtaccaaaga gcaactggtg aacacttacg acaacacgct ggtttacgtc gactatattg
     3661 ttgataaagc gattaatctg ctgaaagaac atcaggataa atttaccacc agcctggttt
     3721 atctttctga ccacggtgag tcgttaggtg aaaatggcat ctatctgcac ggtttgcctt
     3781 atgccatcgc cccggatagc caaaaacagg tgccgatgct gctgtggctg tcggaggatt
     3841 atcaaaaacg gtatcaggtt gaccagaact gtctgcaaaa acaggcgcaa acgcaacact
     3901 attcacaaga caatttattc tccacgctat tgggattaac tggcgttgag acgaagtatt
     3961 accaggctgc ggatgatatt ctgcaaactt gcaggagagt gagtgaatga aaattctgat
     4021 tgttgaagac gatacgctgt tattgcaggg actgattctg gcggcgcaaa ccgaaggcta
     4081 cgcgtgcgat ggcgtgacaa ccgcgcggat ggcggaacaa agccttgagg caggtcatta
     4141 cagcctggtg gtactggatt tagggttacc cgacgaagat ggactgcatt ttctcgcccg
     4201 tatccggcag aaaaaatata ccctgccggt actgatcctc accgctcgcg atacgctgac
     4261 cgacaaaatc gccgggctgg atgtcggtgc cgacgactat ctggtgaagc cttttgcgct
     4321 ggaagagtta catgcccgta tccgcgccct gctacgacgc cataataatc agggcgaaag
     4381 tgagctgatt gttggcaatc tgacgctgaa catgggtcgc cgtcaggtat ggatgggcgg
     4441 tgaagagttg attctgacgc ccaaagaata tgctctgctg tcacggttaa tgctcaaagc
     4501 aggcagtccg gtgcatcggg aaattctcta caacgacatc tataactggg acaatgaacc
     4561 ctcgaccaac accctggaag tgcatatcca caatctgcgc gacaaagtgg gcaaagcccg
     4621 tatccgcacc gtgcgcggct ttggctatat gcttgtcgcg aatgaggaaa actaattgaa
     4681 tctgatgcgt tttctgcgcc gaccaatatc gctgcgccaa cggctgatat tgaccatcgg
     4741 ggccattttg ttggtgtttg agctgatcag cgtcttctgg ctatggcatg aaagtaccga
     4801 gcagattcag ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc gccacatcat
     4861 gcgtgagatc cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta tggtcagcct
     4921 gacgctattt atctgctatc aggcggtacg ccgcatcacc cgcccgctgg cggagctgca
     4981 aaaagagctg gaagcgcgca ccgccgacaa cttaacgcca attgccattc acagcgccac
     5041 cctcgaaatc gaagcggtgg tttcggcgtt gaacgatctg gtcagtcgcc tgaccagcac
     5101 gctggataac gaaaggttat ttaccgctga cgtcgcgcac gaactgcgaa cgccactggc
     5161 gggggtgcgt ttgcatctgg aactgctggc gaaaacgcat cacattgatg tagcaccgtt
     5221 agtggcacgg cttgatcaga tgatggagag cgtctcccag ctgctgcaac tggcgcgtgc
     5281 cggacagtca ttttcttccg gtaattatca acatgtaaaa ctgctggaag atgtcattct
     5341 cccctcgtat gacgaactca gtaccatgct cgaccagcga cagcaaaccc tgctattgcc
     5401 agagagcgcc gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga tgttattgcg
     5461 aaacctggta gaaaacgccc atcgttacag cccgcaaggc agcaacatta tgattaagct
     5521 gcaagaagat ggcggagcgg tcatggcagt tgaagatgaa ggaccaggta ttgatgagag
     5581 taaatgcggg gagttgagta aagcgtttgt acgtatggac agccgttatg gcgggattgg
     5641 tctggggtta agtattgtca gccgcattac acagttgcat cacgggcagt ttttcctgca
     5701 aaaccggcaa gagacttccg gcacgcgggc ctgggtacgg ctgaagaaag atcagaacgt
     5761 ggcaaaccag atataaagaa agctgctgac caccagcacg ctgaacacgg tagttaaact
     5821 ttcataaaca cggtttttca ttgcactctc ctccgttaac ctggaggaga gtatgcgcgt
     5881 cagagattaa gtgaacctta agagttcaat tggcctgata agacagcgtc acatcaggcc
     5941 atccgtttca gcttattcat caattcgcgg atgttgctgc accaggcggg tacgtttcgc
     6001 ctgcaaatcg gcaatctcgt ggtcaatatc atcgattttc tgctcgatat tatcgtaatg
     6061 ctcgacgaga atttccttcg cttcctgtat atctgacgcc gccggtgtcg cacctttcaa
     6121 cggacgattc gccgtctctt tcatggttac gccagtgatt aaaccaacca ccgccactac
     6181 catcaggtaa taggcaggca tcatcagatt ctgcgagctt tcgaccagcc aggccgccag
     6241 tgttggcgtc agaccggcaa ccagcaccga aatattaaat gccgccgcca gcgcgctgta
     6301 acggatatgc gtcgggaaca tcgctggcaa ggtagaagcc ataacgcccg taaagcagtt
     6361 aaggatcacc gccagcatca gtaacccggc aaaaatcaga ccgatgacgt tactgttaat
     6421 cagaataaac gccgggatcg ccaacacaaa cagggcaaca ctaccaagta gcacaaacgg
     6481 acgacggcca aaacggtcgc tcagcaagcc catcaccggc tggacaaaca gcataccgat
     6541 cataatggcg ataataatca gcaccccgtg gtcttcggag taatgcaggt tatgcgacaa
     6601 ataactcggc atataggtca gcaacatgta gtaagtcacg ttggtggcaa ttaccagacc
     6661 aatacatgtc aacaggctgc gccagtattt agtggcaatc tctttaaacg agactttcgg
     6721 gccatcctgc aaaccttcgc ggtcgccctg ttccagttta tcaacatgct gctggaacgc
     6781 cggagtttct tccagcgcat ggcgcaggta aagcccgata atccctaacg gcagagcgat
     6841 aaagaacgga atacgccagc cccagtcgag gaagttctct tcgccgacaa tggtcgaaat
     6901 taacaccact acgccagctc ccagcacaaa cccggcaata gaaccgaagt ccagccagct
     6961 gcccataaag ccacgtttac ggtcagggga gtattccgca acaaatattg acgccccggt
     7021 atattcaccg ccgaccgaga aaccttgtgc catcttacag atcaacagca gaatcggtgc
     7081 ccaaatacca atcgtgtcgt aggacggtat taagccaata cagaacgtac tgatcgacat
     7141 aatcacaata gtgatagcga ggatcttctg gcgaccatat ttatcgccca acataccaaa
     7201 gaagagtccg ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa gtgcagcaac
     7261 catctgcacg ctggggtcag cccccgggaa aaaaacttta cctaatgcgt aagcaacaaa
     7321 accataaaca ccaaaatcaa accattccat tgcattaccc agtgatgctg cggtaatggc
     7381 tttacgcagt ttaccgtcat caataatggt gacatcacga agggtaattg gttttacttt
     7441 tttccttttc agcatagctt tcctcgcaga gttggcccat ttccgcaaac gccagaaaaa
     7501 tcagcgcgac ggccgcaaaa ggggagcggg aattgcagga tgcaatctct gtaacaagcg
     7561 tagcgggttt gcttacaccc tccggttaag gaatgtacaa tcaaaccagt tgacgcctga
     7621 attgggcagt tagtgacctt tttaccctac cagtgtttaa atttgtgatc aacttcacac
     7681 atggatttcg tttaggactc attgatgtaa ctaattattt acgccaaatt tacagagaag
     7741 atttcctcga ccattgcccc caccaaacgc tgcgctgaac agagtcgtgg catccccagc
     7801 gcgacagttt gccaacgttg ggtcactgac cagcaaacgg gttgtttatc tgccgtggtc
     7861 cactttccca gccaacttaa aaaatcagca tgatccccgc ccgtcggaac ggtcgaagag
     7921 gcaaaccact gatggtaata gtgacgcgtg gtcggggccg cattgatact ctctgccaga
     7981 tgttgtgtcg ctttcacgcg caaatcaccc agcaacaacg ccaccgcttg cgggcttccc
     8041 agacgacagg catcgccaaa tgccccccaa cgcaactctt cgagaacgat aaaacagcgc
     8101 ccaggcaacg accaggcgtt atacgcctca gcacgccagc gggtaaaaat ttgctcgctc
     8161 tgttcacccg cctgcaccag taggcctcgt tgcgttaacg ccttttcctt ccagcgacaa
     8221 cgggctgaaa accactcata caaggcggca atttgctggg ggagctccgg gaaagcagag
     8281 tgctggcggt gatccatttt ttgcagcagc gccagatgct gcgcgatctg cgtcagcgcc
     8341 agatgaccgg gatccagcat cgacgccagt ttttccacca atgtcaaacg caggctaagc
     8401 ggcgcattta acatgccgcc gttcgtccat gtctgaagct gcacgcgcgc aagaagttcc
     8461 tgcatcagtc gttcacgaaa ctgctgctga tgggcttgtg gaaggcgggc atcatcgccc
     8521 tgcgccagat ccactaaaaa gcggggataa accgactcca gcacgcgacc ggggccgtcc
     8581 agtaacgtct tggtcaatat cgttctgccg tgaaaagtgt ttgaatatca tcgcgtaaca
     8641 gctgggcgtc ggtgtaaatc cagccgtgag tcatcacagt ctgctgcaat tgctgctgca
     8701 tcagcctgac caccgattca ttttgttgac gcagagccag actttcgcgt aaacgcgtct
     8761 gtaattccgt caaacatgaa gcgaactcag cgaaaaaagt attcatgcct gccgtaacag
     8821 attcatcgac ctgctctgcc agaactttag ccatttgttg gcaataaaga tcgacttctg
     8881 cgcttaatgc tccttgcaac acactgtaat caaccgtttc tgtcggggat ttctcattcc
     8941 cccgtcccca gtcgggctga ttcaaccagc gcgaaaaagt ctcacgcaca acgcctaaac
     9001 gcgtgctctg ctcgtccgtt gcttcctggc gcgaaatgac ttcactgaac agctggcgag
     9061 tgttgaagtg gggaactgcg ccgtgaaaaa caggaaaatg aaacccggga cgaaaccctg
     9121 actcgctcaa ttccattttg acttgttgtt caatggggcg aataacatcg gttaacaccc
     9181 ggcaaagagt ggattcaagc tcggcaaaac gcagcgtaaa gtcgcgactg atggtgttct
     9241 gcgccgtctg taacagtgtc tcacagcggg tacgaatctc gcttaacggc tccgaatcat
     9301 cctgaaacaa ggcggctaac tgcgccttca gcgcatcttg ctgttgacgc agaaagaggt
     9361 tggcggaggt cagggccagc tcgatttcat gtttaatctc gccgctcacc tgcgcctgat
     9421 tgagttgcaa tagctgcaaa ctttcttcga tctggtggat attttgccgc aattgttcac
     9481 aagcgacgtt taacccgtgc gcacgaaaat ccaggtattc ccgcgcctgc tgcgcgtaat
     9541 tcaacagttt atgcgcggca gatcgcagag catacaacga ggcgttagca taagcggcat
     9601 gaagcaacgc ctgaattggc tgggcgaaca gcgaatcttc ccacaactga tcggcagcat
     9661 gacgaatatg ttcgaggtcc gccagatcgg catgacgcca gcgcctgccg agcgcggcat
     9721 gggcaaaatc ttccacccag cgttgttgct ctggcgctgg taacttatcg ttgttggcta
     9781 actcatggcg cgcccgattc gccaggtagc cccacatcga cgacaccgga aatatctgct
     9841 gtggcgtaat acagcctttc atcagcgtcc cggaaatcag tgcccgcacc tggtcggcgt
     9901 cgtcactgtt acgatcctgt tgatcgaact tattgaccag cacatacagc ggcaccgatt
     9961 gccccaccgc caaaatcgcc tcacggacct cttcatcgga gatcgatttc agttgcgtat
    10021 aatccagcac cgccagtacc gccgaggcgc gtgccagctg ctggttaagc attttttgca
    10081 gatgcggttg cccggcttca tttggcccgg gggtatccag taacgtcaac tgaccgggat
    10141 aactctccag ccccgccaga tggacaaact ccacttcaat cacgggaata tgctcaatgg
    10201 cggcgtaagc agaaaaagga aaatcgacgt ccagcgcctt cgccagtcgc accaaatcat
    10261 tcaaactttt cagacaatga aaaataggct gggcacccag ataatattta tcgaaagcga
    10321 cgccattttc gatccgctgc ataagcgcac gcatatcttt atctatttcc agcacatcgg
    10381 tcagatgctt aatatcgcaa tcacgcaggc gctgttgtaa tttttgaatt aaacaatcga
    10441 ttggtgcaac atgtgaaaaa tgcagtaccg gttctttttg cccgggcgta tggcgaataa
    10501 gcgtcggcag cgcagtcatt gggcgattac gattaggcag aacctccgta ccaacaatgg
    10561 cattaatggt ggtggatttc cctgctttca tagtaccgac aatcgccagc accatttcca
    10621 gtcgggaaat tttacgcaac tcattattca gcatcgcgtg acgctcggcg atattaggct
    10681 gactccaggg taaagcccgt tgtggcgcat cgtctccggg tacagagaga ggcatttttt
    10741 ccagtaactg caactgttgg cgagaaagct gtaacaggcg ttcagcctcc tgacttaact
    10801 c
//
LOCUS       DAZUCE010000177        10727 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZUCE010000177 DAZUCE010000000
VERSION     DAZUCE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.177"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            109..822
                     /gene="yghS"
                     /locus_tag="ACHY03_003790"
     CDS             109..822
                     /gene="yghS"
                     /locus_tag="ACHY03_003790"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS3906970.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCRSQQGHLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            854..1612
                     /locus_tag="ACHY03_003791"
     CDS             854..1612
                     /locus_tag="ACHY03_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906971.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1658..3007
                     /locus_tag="ACHY03_003792"
     CDS             1658..3007
                     /locus_tag="ACHY03_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFS3906972.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAAHELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            3007..3831
                     /locus_tag="ACHY03_003793"
     CDS             3007..3831
                     /locus_tag="ACHY03_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906973.1"
                     /translation="MMRKYFPLEASEHLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            3843..4403
                     /locus_tag="ACHY03_003794"
     CDS             3843..4403
                     /locus_tag="ACHY03_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFS3906974.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4434..5504
                     /gene="lptF"
                     /locus_tag="ACHY03_003795"
     CDS             4434..5504
                     /gene="lptF"
                     /locus_tag="ACHY03_003795"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFS3906975.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFTLLQKFSR"
     gene            5501..6580
                     /gene="lptG"
                     /locus_tag="ACHY03_003796"
     CDS             5501..6580
                     /gene="lptG"
                     /locus_tag="ACHY03_003796"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFS3906976.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLVALSLVYWYDRQH"
     gene            complement(6615..7787)
                     /locus_tag="ACHY03_003797"
     CDS             complement(6615..7787)
                     /locus_tag="ACHY03_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS3906977.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAAFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFAILKQ"
     gene            complement(7787..8035)
                     /locus_tag="ACHY03_003798"
     CDS             complement(7787..8035)
                     /locus_tag="ACHY03_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFS3906978.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8067..8981)
                     /locus_tag="ACHY03_003799"
     CDS             complement(8067..8981)
                     /locus_tag="ACHY03_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFS3906979.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGIAGGYDW
                     QRVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8978..10666)
                     /locus_tag="ACHY03_003800"
     CDS             complement(8978..10666)
                     /locus_tag="ACHY03_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFS3906980.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFSCQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PDDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRIAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
BASE COUNT         2581 a         2682 c         2809 g         2655 t
ORIGIN      
        1 cgtaattcag gtcaatgtca caattaacca ttgtcacaat aaggttgaac ggatattctg
       61 agcacggacc acgtaatatt caatacatta tttactgccg ggtttttcgt gaatcaacgt
      121 aatatgtcaa taattaattc cacgccagtg cgtgttattg ccattgtagg atgtgatggt
      181 tcaggtaaat cgaccctcac ggcaagcctg gtaaatgaac tggcagcaag aatgccaaca
      241 gaacgcattt atctcgggca atcgtccggg cgaattggcg aatggatttc acagctccct
      301 gttattggcg caccttttgg gcgttatctg cgaagtaaag cggcacatgt gcacgaaaag
      361 ccctcaacac cgcctggcaa tattactgcg ctggttatct atctgctttc ctgctggcgg
      421 gcgtacaagt ttcgcaaaat gttgtgtaga agccagcaag gccatctgct catcaccgac
      481 cgctacccgc aggttgaagt gccgggtttt cgctttgatg gcccgcaatt ggcaaaaacc
      541 acgggcggta acggttggat aaaaatgtta aggcagcgcg agttgaagct gtaccaatgg
      601 atggcgtctt atttacccgt attgttgatt cgtcttggca ttgatgaaca aaccgcgttt
      661 gcgcgtaaac ctgaccacca actggcagcg ttacaggaaa aaatcgccgt tacgccgcaa
      721 ctgacattca atggcgcaaa gatccttgaa ctggatggtc ggcaaccagc cgatgaaatt
      781 ctgcaagcgt cactacgcgc aattcacgcc gcgctttcct gatcaccaaa atggataggg
      841 gatgatttaa cgaatggatg cactacaaac acaaactgtt aatagtacaa ccgcgccgca
      901 gcccaactac attccggggc tgattgcggt ggtcgggtgt gatggcaccg gtaaatccac
      961 actgaccacc gacctggtga aatcgctgca acaacactgg caaaccgagc ggcgctattt
     1021 ggggctgctc tcgggcgaag acggcgacaa aataaaacga ttgccgttgg ttggcgtctg
     1081 gctggaacgg cgactggcgg ccaaatcctc gaaaacccaa agcatgaaaa ccaaatctcc
     1141 ggcgctatgg gcggcggtga ttatgtactg cttctcgctg cgaagaatgg cgaatctacg
     1201 caaggttcag cgactggcgc aaagtggcgt tctggtggtc agcgatcgct tcccgcaggc
     1261 tgaaatttcg ggcttttatt atgatggacc ggggattggc gtcgaacgtg cgaccgggaa
     1321 aataagcatg tttctggcgc agcgcgaacg gcgtttatac caacaaatgg cgcaatatcg
     1381 cccggaatta attattcgcc tgggcattga tattgagact gccatctccc gcaagcctga
     1441 ccatgactat gccgagctgc aggacaaaat cggtgttatg tcgacgattg gctataacgg
     1501 cacaaaaatt cttgagattg attccagagc gccctacagc gaagtgctgg aacaggcaca
     1561 gaaggcggtt tctctggttg ccatcgtttc tgaccgccga agtttaactt aaggctggcg
     1621 ggatactttt tactatcaac gataaggtca ggctgaattg gcgggtttta acatcaaaca
     1681 ttggtttgca gatggcgcgt ttcgcaccat tattcgcaat agcgcctggt taggctccag
     1741 taatgtcgtg agcgccttgc tgggtctgtt ggcgctctcg tgtgccggta aagggatgac
     1801 gcccgccatg tttggcgtac tggtgattgt acaatcgtac gccaagtcga tcagcgattt
     1861 tattaagttt cagacatggc agctggtggt tcagtacgga acaccagcat taaccaacaa
     1921 taatccgcag caattccgca atgtcgtctc attttccttc tcgctggata tcgtcagcgg
     1981 cgcggtggcg attgtcggcg gcattgcctt actgccattc ctttcccatt cattaggtct
     2041 ggatgaccaa agcttttggc tggcggcgct gtattgcacg ctcattcctt caatggcttc
     2101 ctccacgccg accggcattc tgcgtgcggt agatcgcttt gatttgattg ctgtacagca
     2161 ggcgacgaaa ccttttctgc gcgcagcggg gagcgtcgca gcctggtatt ttgactttgg
     2221 ttttgcgggt tttgttattg cctggtacgt gtcgaatctg gttggcggca ccatgtactg
     2281 gtggtttgcc gcgcacgaat tacgccgcag aaatatccat aacgccttca aattgaatct
     2341 gtttgagtct gcccgacaca ttaaaggcgc gtggagtttt gtctggtcaa ccaacattgc
     2401 ccactccatc tggtcggcgc gtaactcgtg cagcacagtg ttggtgggga tcgtgttagg
     2461 acccgctgcc gccgggttat ttaaaatcgc catgacattc ttcgacgccg ccggaacgcc
     2521 agcgggtttg ctgggtaaaa gtttttaccc agaggtgatg cgtttagatc cgcgcaccac
     2581 cagaccgtgg ttgttaggcg tgaagtccgg tttactggcg ggcggaatcg gtattctggt
     2641 ggcgctcgcg gtgttgatcg tcggcaagcc gctcatttcg ctggtgtttg gcgttaagta
     2701 tctcgaagcg tatgacctga ttcaggtgat gctgggcgca attgtgatct cgatgctggg
     2761 cttccctcag gagtcattgc tattgatggc gggaaaacag cgcgcttttc tcgtggcgca
     2821 aaccatcgcc tcaatcggct acatcgtatt gctgttcatg ttctgtcatc tgtttggcgt
     2881 gctgggagca gcgtttgctt atttcggcgg tcaatgtctg gatgtggtgc tttcgctaat
     2941 tccgacctta aaggcatttt ttcagcgcca ttccttgctt tataacgcag ccggagagaa
     3001 atcctgatga tgagaaaata tttcccgctt gaggcgagcg agcatctatt tgttgcgatt
     3061 gaggaagatg atgttgttga tgcccaggtc agtttgccgc cgactatcgc actaagttgt
     3121 accaccgaaa tcatccatga taattacgcg ttatgcctgc aattctggct caatggcgtt
     3181 gatcgtcagg aattacttcg tttaattcgc aaacaagcca aaggcgatga gctgacgaca
     3241 gacgagcgta aacagtttaa atacatgcga gcgcgctaca agcacctgcg ttttgcgcaa
     3301 cggctgtatt taaagaagca tcaggccgga tttttattcg gcaaaacgac cgtttttctg
     3361 ggacgttttc aggacggatt tcgtaacggt aagaaaaata ttgtgtcgta ttacggcaac
     3421 ctgttacgaa tttatttaag ctctccggtc tggtcgttgg tgaactattc attacgccat
     3481 agccagttag aaagcgtaag cggttttatt gcctatcggc aaaagcaaat gcataccctg
     3541 aaagagatta ttgccaaacc gcgactaacc ggcagggaat ttcatgatgt gcgaaaaatc
     3601 atcagccagc aggtttctta ttacgacacc ttaagatcgc ttgatcctga aaataaagag
     3661 gcgttacaga tttcccgttt cctcgccgcc attaatggcc tgatggggga taaacacgac
     3721 gacatggtgg cggatgatat ggaaaaccgc cagtcgtatg atgcaccggt ggcgctggat
     3781 agcgatattc gtcagcgctt agagctgctg atttcgcgtt ttccgctata accggagcaa
     3841 tgatgtcatc tcgtttgatt attgcgctga ttatcatgct cttagccccg ggtgtgcagg
     3901 cacacaattt cgtcactggc aaaacggtta cccctgttta tattcaggaa ggcggcgagc
     3961 tgcttctgaa tagcgacgac gaaattcact accaaaagtg gaacagcaca caattggcgg
     4021 gaaaagttcg cattattcag tatatcgccg ggcgtaagtc ggcgaagaaa aagaactcac
     4081 tgttaattaa agcggttgaa gcggcaaact ttccgcagga tcgttttcag cccaccacca
     4141 ttgtgaacac tgatgatgcc atctttggta ccggctattt tgtcgttggc aaaatcgaaa
     4201 aaaataaacg ccgctatccg tgggcgcaat tcgttattga cggcaatgga cagggacgcg
     4261 ttgcctggcg cttgccggag cagagttcaa ccattctggt gctgaataaa gccgggcaaa
     4321 ttcagtgggc aaaggacggc agcctgacgc cggaagaggt agatcatgtc attgcgctgg
     4381 cgcagaaact gatcaatgaa taaaaaattc aaaggattga gggcgttagt gagatgaagc
     4441 tggttgaaca ctacatcatg cgtggtacgc gccgtctggt gctgattatc gtcggttttc
     4501 tgatctttat tttcgccagc tactccgcac agcgttatct gaccgaagcg gcaaatggga
     4561 cattagctct tgatgttgtg ctggatatcg ttttctacaa agtgctgatt gcactggaga
     4621 tgttgttacc tgttggtctg tatgtgtcag ttggcgtgac gctagggcag atgtataccg
     4681 actcggaaat taccgctatc tctgcggcgg ggggcagtcc gggacgcttg tacaaagccg
     4741 ttctttatct ggcgataccg ctaagtattt ttgtcaccct tctgtcgatg tatggtcgtc
     4801 cgtgggctta tgcgcagatt tatcaactgg agcagcagtc gcagtcggag ctggatgttc
     4861 gccagttgcg ggcaaagaaa tttaacacta acgataacgg acgaatgatc ctttcgcaga
     4921 cggttgatca ggataataat cgcctgactg acgcgctgat ttatacttct actgccaatc
     4981 gaacccgcat tttccgcgcc cgttcggttg atgtggttga cccatcaccc gagaaaccga
     5041 ccgttatgtt gcacaacggc actgcctatc ttctcgatca tcaggggcgt gacgacaacg
     5101 aacagatcta ccgtaatctg caattacatc tgaatccgct ggatcaaagc cctaacgtca
     5161 aacgcaaagc gaaatcggtc acggagctgg cgcgctccgt ctttccagcc gatcatgccg
     5221 aactgcaatg gcgacaaagc cgtggcctga ccgcattgtt gatggcgctg ctggccattt
     5281 cattaagtcg ggtaaaaccg cggcaggggc gattttcaac gttattgcca ctgacgttgc
     5341 tgtttattgc cattttttat ggcggcgacg tctgccgtac gctggtggct aacggcgcga
     5401 ttcctctcat tcctggtttg tggttagtgc ccggactcat gctaatgggc ctgctgatgc
     5461 tggttgcacg cgacttcact ttgctgcaga aattttcccg atgaatgttt tcagtcgcta
     5521 tttaatccgt catctctttc tcggttttgc cgccgccgca gggctattgc tgccgctttt
     5581 taccaccttc aacctgatta acgaactgga tgatgtcagc ccgggcggtt atcgctggac
     5641 tcaggcggtg ctggtggtgc taatgacctt accgcgcaca ctggtcgaac tttcgccgtt
     5701 tatcgcctta ttgggaggga ttgttggcct ggggcagtta tcgaaaaaca gtgagcttac
     5761 cgccattcgc agcacggggt tttctatctt ccgtattgca ttggtggcgc tggttgccgg
     5821 gatattgtgg actgtttcgt taggcgcgat agatgagtgg gtagcgtcgc cattgcagca
     5881 acaggcgctg caaatcaaat cgaccgccac tgcgttgggg gaggacgatg atattaccgg
     5941 caatatgctg tgggccaggc gcggcaatga atttgtgacg gtgaaatcgc tgaatgaaca
     6001 gggccagcct gtgggcgtgg agatatttca ttatcgcgac gatctctcgc tcgaatccta
     6061 catttatgca cgcagtgcca ccattgaaga cgacaaaacg tggatcctgc atggtgtgaa
     6121 tcataaaaaa tggctgaatg gcaaagaaac gctggaaaca ttagataatc ttgcctggca
     6181 atcggccttc accagtatgg atcttgaaga gttatcaatg ccgggcaata ctttttctgt
     6241 ccgtcagctt aatcattaca tccattattt gcaggaaacc gggcaaccca gcagcgaata
     6301 ccgccttgca ctgtgggaaa aactggggca acccatcctg accctggcga tgattttgct
     6361 ggctgtgccg tttaccttta gcgccccgcg ctcgccaggg atgggtagcc gtctcgctgt
     6421 aggtgtcatc gttggcttac tcacctggat cagctatcaa atcatggtca atctgggatt
     6481 gctatttgcg ttgagcgcac ctgttaccgc actcggttta ccgatagcgt ttgtgctggt
     6541 ggcgttgagc ctggtgtatt ggtatgacag acaacattaa acccgccgtg gcgcaacacc
     6601 acggcgggct cacattactg cttcagaata gcaaatgcct gaatgatggc atcgatttgc
     6661 tcacgactgt gggcagcgtt tacgctacag cgcagcagcg tgatgcccgc cggcgcggct
     6721 ggtggcagga taaggttcac atacacgccc agcgaaataa gctcacgcca gatgcgtaaa
     6781 ccttcctctt tcgaaccgat tattaccggc actaccggac taatatgtga gcttaactca
     6841 taacccagct tttgcagccc gtcatacaga tggttggcat tatccatcag cttttgccgc
     6901 aactctggtt gagtggcaat agttcttaac gacgagcgca cggttgcaat gcaggaagga
     6961 gaaggggagg cggtaaaaat atacggacgg ctgccgtagc gcagcacttc catcgcttcc
     7021 gagcctacag caaaaccgcc aatcgaggcc agacttttac tgaaggtgcc aacgataata
     7081 tccacatcat cttcaacgcc gacagcttca gccagtccgc gccccgttgc gcctaacacg
     7141 ccaaacgaat gcgcttcatc gacaatcaaa taaccaccga gacgacgttt gatatcaaca
     7201 atttccgcta acggcgcaac gtcaccgagc atgctgtaga taccctcaac gatgataatc
     7261 gcctctttcg cccgttcacc gaggcgaacc atgcgccgtt ccagatcttt agcatcgtta
     7321 tggcggaaac gaataatttc cgcgccaccg agagaacagg cgtcataaat actggcgtgg
     7381 ctgtcggcat ccagtaacac cacggcgtta tggtcggcaa gtgcgctgat gacgcctaaa
     7441 ttagcggtat aaccggttga aaaaacgata gcggtagggc gattgaaaaa cgcggcaatc
     7501 tctttctcta acgccaggtg cggcgcataa ctgccgtttg ccatacgaga accggtggtg
     7561 ccggtgcctt gggccgctaa cgctgcctga ccttcggcga tggcatcatg attaaaggtt
     7621 aaaccgagat aattattggt cccggccaga atcacttttt tattgccgat ccggccttcg
     7681 gtggcggaat acacttcatc aatacaggtg ccaaacggtg ttaacccgtt gtcactaaat
     7741 tgcagtcgct caccagcaag gcgcgcatat ttatcgtata gccccattat ttattctcca
     7801 gccatggaag tagagtttcc attaattgcg caggggtttt gacatccagc agaatattaa
     7861 taggaatgga aatatcgaat ctgtcttcca gcatcattaa gagatccatc actttgatcg
     7921 actccaggcc aagatcgttg acgagatcgc tgtcgggctt aatttccaca ccgttttcga
     7981 ccaaatcctg taaacaggaa aggatgtaat ccattactat ttcacgattt accatagaaa
     8041 cgttaacaca cctttaaaca agacaatcaa aatagcccgt tgcgtaatgc gtgttccagg
     8101 ctaatcccgg gaaaccaatt aatatcttca gaaatacggt tattacttgc cgaccagtcg
     8161 gcgtgggtta attcacgaat tttcgagcgg gtcagcatag gttctttacc tgccaggcgg
     8221 ctcaacgcgg tactaatatc cgcaaggcag gtgaggaccg gcagaggaat accaaccatt
     8281 cgcacggaac cacagcgggc gtcggcggca agctgtcgta cgcgttgcca gtcatagccg
     8341 ccagcgatgc catcgcataa ttcataggtt tgcgtctgta cagtttcggc gcttaaccac
     8401 tgacccactg cttgcgcaaa atcggtgaca tgcaggaaag agagctgtgt atctggtgca
     8461 ccaagtcgtg gcagcaggcc gcgcagcatc cagtcaaaca gcggttttaa ctctttatcg
     8521 ccgggaccat acacggctgt cgggcgaaaa acgcccagcg taatttcgtc agccattgcc
     8581 gtcagccgtt gttcggcgac gtgtttggaa tttgcgtacc aggagagctc tggatggcgc
     8641 gccgccagcg aagagataaa cagaaaacgt tggcaaaagc cgctctcttt tgctgcttgc
     8701 atcaggcgca ggctgccgtc aacgttacag tgggtaaaaa tctcttcttt gtgtccgcgc
     8761 acttgcccgg cgcaatggac caccgcactg gctccggcaa ccagctcgct aagtgaatga
     8821 gtatcttcca gcgaaccgcg cacccaagtg agattgtcat tgacgtgagc gcgggcagta
     8881 cgcgtcaatg cgcgaacgtg aaagccgcgg gcgagcaggt tatcgataat atatttaccg
     8941 ataaaaccgg tagcgcccgt caccgcgaca gtttgattca tgccagggat tcctgcacat
     9001 gaaggcttgc agcataagcc ttctgataac gttttttcgc ttccgcacgg gcaggcttgc
     9061 cggaggacgt tcggggaatg ctgtggggcg gcaacagatc gatagccgct gttacgccaa
     9121 attcgctttg gatccgtgct gccagcgcgt ggataagctg tccgcgacgt tcttcatcgc
     9181 tgatccgaca ctggatctgc aaaatgattt tttcctgggc ggtaacaaaa gcaatcgcat
     9241 cgccagaatg aatttccggt tcctgttccg ctatatattc aatgtcctgc ggccagatat
     9301 tacggccacg aataataatc agatctttaa tgcgtccggt gacatacaga taaccgtcca
     9361 gcagataacc gaggtcgccg gtgtctaacc agcccgtcgc ggcaatctcg tcttgcgaag
     9421 cctggtcgcc aaagtaaccg ctcatcagac tgggaccgga gatgcaaata tggcctacca
     9481 cacgttccgc gaccggcata cctgcttcat tgcggatttc aatgccatgt tccggcaacg
     9541 ctttaccgca gttgacgaaa gtcgatacgg cgcgtgtctc tgcacccggc gcaacggctt
     9601 taccctgata ttcgaggatg tcgcgatcca cttcgttaac cacaaccccg gaggcttcat
     9661 cagagaaact gacagccagc gcattttctg ccagtccgta gcacggcatg aaagttttat
     9721 tgtcaaagtt aacctgacgg aaacattcag cgaattgatg gagttgttct gcggagatgg
     9781 gttctgcacc aataccagcg atgcgccagc aggaaagatc cagttcagcg agatcttttt
     9841 cattcacgcg gcgctggcac aattcatagc caaacggcgg cgcaacggaa acggtgccgc
     9901 gatttttact gatcaattta agccattgca gaggacgcat ggcaaaatcc tgagtgcgca
     9961 aataatctac tgaaagttgc gtggcgacgg gggtcaggag aaagccgact agtcccatat
    10021 catggtagaa aggcagccag gagacgcagc ggtcgccagg gcgtaattta atgccgtcgt
    10081 ggcttatagc acgtagatta gccatcactt cgcgatgggt gataatgacg ccacggggaa
    10141 aacgggtgct gccggaggtg tactggaggt aggcgatatc gtccggaact gggcgctgga
    10201 gcgcaacatc agcttccggt aatgccttaa accaggcatg gcttaataca tgcaattcgg
    10261 ggttgttatg cgtggcggca ttgaccagcg gcaaccactc atcaccagtg ataatggcag
    10321 cgggctggca acttgccagt aaaccctgca gtttggcgct ccaggaatcc cgctgaccga
    10381 cgcccattgg aatcgccaac gggacggcga ctaagccggc atactggcag gaaaaaaaag
    10441 cctctacgaa cccgctactg gtttcggcaa tcagcgccac gcgatcgcct tttttcaggt
    10501 ttagcgataa caaccgcttc gcaccagctt cggcacgcgc ttttaacgtc tgatattcca
    10561 gttgatcttc aagttggcaa cgtctgtcat aaaaattcat tccagcgcta ctcagagcgg
    10621 cgtagtccaa agcatcaacc agcgttggaa aatcggcata gcgcataggt agggaatgcg
    10681 taaagatttt attagacata tacaccacga aaaaattgga tatccaa
//
LOCUS       DAZUCE010000178        10659 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZUCE010000178 DAZUCE010000000
VERSION     DAZUCE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.178"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            127..1035
                     /locus_tag="ACHY03_003801"
     CDS             127..1035
                     /locus_tag="ACHY03_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFS3906981.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1254..3245)
                     /gene="tkt"
                     /locus_tag="ACHY03_003802"
     CDS             complement(1254..3245)
                     /gene="tkt"
                     /locus_tag="ACHY03_003802"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFS3906982.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3523..4281
                     /gene="loiP"
                     /locus_tag="ACHY03_003803"
                     /gene_synonym="yggG"
     CDS             3523..4281
                     /gene="loiP"
                     /locus_tag="ACHY03_003803"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFS3906983.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4487..5407)
                     /gene="speB"
                     /locus_tag="ACHY03_003804"
     CDS             complement(4487..5407)
                     /gene="speB"
                     /locus_tag="ACHY03_003804"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFS3906984.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5543..6274)
                     /locus_tag="ACHY03_003805"
     CDS             complement(5543..6274)
                     /locus_tag="ACHY03_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS3906985.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6420..8396)
                     /gene="speA"
                     /locus_tag="ACHY03_003806"
     CDS             complement(6420..8396)
                     /gene="speA"
                     /locus_tag="ACHY03_003806"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFS3906986.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAERLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8405..8536)
                     /gene="yqgB"
                     /locus_tag="ACHY03_003807"
     CDS             complement(8405..8536)
                     /gene="yqgB"
                     /locus_tag="ACHY03_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFS3906987.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8630..8773)
                     /gene="yqgG"
                     /locus_tag="ACHY03_003808"
     CDS             complement(8630..8773)
                     /gene="yqgG"
                     /locus_tag="ACHY03_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFS3906988.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8884..9135)
                     /gene="yqgD"
                     /locus_tag="ACHY03_003809"
     CDS             complement(8884..9135)
                     /gene="yqgD"
                     /locus_tag="ACHY03_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFS3906989.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            9191..10345
                     /gene="metK"
                     /locus_tag="ACHY03_003810"
     CDS             9191..10345
                     /gene="metK"
                     /locus_tag="ACHY03_003810"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFS3906990.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2667 a         2837 c         2665 g         2490 t
ORIGIN      
        1 aaacatactt ttctaataca atgttttttc gctatacccc tttatagatt ctgaattctc
       61 acctgtcggc tgttttctcc tcatctcatg tgacatattc ccacacttta ctcaaggaag
      121 attgtcatga aagcacctgt ttcattaacc tcatttcgcc cacaaaaatc tcttgctatc
      181 gccattggcg tgcttgccgt ggttgtgctg ccatttctct cgtattacac cgttaatgaa
      241 ggtgaacgcg gcattttgct gcgctacgga aaaatcgtaa aagttgccga gccgggtctg
      301 ggttttaaga tcccgtttat ggaaagcgtg gagaaaattt ccacgcgtaa tcaggctgtg
      361 gtctatcagg gattacaggc ctacagccgc gaccagcaac cggcacaaat gacagtatca
      421 gtcagcttcc acattaaacc ttcagaagca ggcgcggttt ataccaccta caacaccatt
      481 gaagcattaa aagatcgtct gattgtccgt cagttgccaa ctcaactgga gaacgttttc
      541 ggccaatata ccgccatcag cgctgtacag gatcgtacaa aactggttca ggatctgcaa
      601 aatgccatgc gcaaagccgt tgttgggcca gtggtaattg acggggtcca gattgaaaat
      661 attgattttt ctgacgctta tgaaaagtcg attgaagacc gaatgaaagc agaagtcgcc
      721 attgccacgc gtaagcaaaa tctggaaaca gagaaaatcc aggcgcaaat agcggtaact
      781 caggcccagg cagaagcaga cagtaaactg gcggcagcca aagcagaagc ggaaactatt
      841 cgcgtaagag gcgcggcaga ggctgaaact attcgtttaa aaagtgcggc tgaagcagaa
      901 gcaatccgcc tgcgcggtga agctctgcgg gataatcccg gacttgtagc cctcaccact
      961 gccgaacgtt gggacggtaa actgcccgat accatgatcc ccggcagcac agttccgttt
     1021 atctctacta agtaaaaaag ggccgctttc gcggcccttg agagtgatgg caaaagcaaa
     1081 actgcctgat gcgctacgct tatcaggcct acatacttcc tgcaatatat tgaatttgca
     1141 cgatcttgta ggtcggataa ggcgttcacg ccgcatccga catgaacaac gcgcacttta
     1201 ccaataatct aaagggccgc tttcgcggcc cttctcacaa aacgtcttac tgattacagc
     1261 agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc aaacagcagc
     1321 tctgccggag cagattcacc gaaggtggtc ataccgacga tagcaccgtt caggccaaca
     1381 tacttgtacc agtagtcagc aatacccgct tccaccgcaa cgcgtgcagt aacagctttc
     1441 ggcaggacag actcacggta agccgcgtcc tgcttgtcga atgcgtcggt agacggcatg
     1501 gaaaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc aaccgccagt
     1561 tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca gtctttcagc
     1621 acataaccac cgcgcgcgat gtttgccaac tgctcttcag tacgttcctg ctgcgccagg
     1681 ttctgacggg agaggatcag cgcggtcggg ccgtcctgac gctcaacacc gtatttccac
     1741 gctaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg ggtcacacgc
     1801 agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag accgatggag
     1861 tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg tacggcgtta
     1921 cgtgcgtatt ccacgaacat caggaaggtg gaagtgtacg gcaggaagcc accgtgcagg
     1981 gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg gatgtagtta
     2041 cccgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt agacggtgcc
     2101 aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc gatagcattc
     2161 tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc gataaactct
     2221 ttcgctttcg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt aaattcagct
     2281 gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc agattctttc
     2341 gcctggcctg cttctttcgc atcccactgc gcatagattt cagacgggat ttcgaacggt
     2401 gcgtatttcc agcccagctg ttcgcgggtc agagcgattt ctgcatcacc cagcggcgca
     2461 ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat ggttttgcac
     2521 atcagcaggg acggtttgtc agtcactgcg cgcgcttctt ctactgcgcg tttgatggat
     2581 gctgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt
     2641 gcggtgtcgt cggtgaacca gccttcaacg tgaccatcaa tagagatacc gttgtcatcg
     2701 tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac ttcgtgggag
     2761 atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg
     2821 tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat acctactgcg
     2881 ttggcaatac cctgccccag cggaccggtg gtggtttcca caccagcggt gtaacccact
     2941 tccgggtggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc ttccatcggc
     3001 agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg gccgttggac
     3061 agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt caggaaatca
     3121 cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg accggatttg
     3181 gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc tttacgtgag
     3241 gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg acgacagcgc
     3301 gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg tcatgtacat
     3361 ggaacatcct tttgctgctt cagaaatctc tggatcatgc tcgcatgttg cgcaatctac
     3421 tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcggttcac ctgcaaacgg
     3481 cgcattttta gaataatcct gaccttgtgc ggaagaaaaa acatgaaaat tcgcgcctta
     3541 ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga ctccaacgga
     3601 ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa
     3661 accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc gccagccaat
     3721 agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa tatcaacggt
     3781 cagccggtaa attacaaagt gtatatggcg aaggatgtga acgcctttgc aatggctaac
     3841 ggctgtatcc gtgtctatag cgggctgatg gatatgatga cggataacga agtcgaagcg
     3901 gtgatcggtc acgagatggg gcatgtagcg ttaggccatg tgaagaaagg gatgcaggta
     3961 gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat tgtcggaagt
     4021 ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca attctcccag
     4081 cgccaggaag cagaagccga tgattattct tacgatcttc tgcgccaacg cggcatcagc
     4141 ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaaac tggaagaagg ccgccaaagc
     4201 tcaatgtttg acgaccatcc tgcatccgcc gaacgcgccc agcatattcg cgatcgcatg
     4261 agcgcggatg ggattaagta aagcctggtg gtgtcgaacg taggtcagat aaggcgttca
     4321 cgccgcatct gacatgaatg gcacatttgt taccttgtgc gcattgccgg atgcgatgct
     4381 ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt tcacgccgca
     4441 tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggcgcttac tcgccctttt
     4501 tcgccgcctg gatatacagc atctccagcg ccagcgttgc cgctgccaga gcggtgattt
     4561 ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacaatg ttgagatctt
     4621 tcaggccgcg taccagttta atggcgcgat cggaggtcag accgccaatt accggcgtac
     4681 cggttcccgg cgcaaatgca ggatccaggc agtcgatatc aaaggtcagg taaaccggca
     4741 tatcacccac aatttgtttc acctgggcga taacgtcatc cacgctgcga tcgttcacct
     4801 ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ttcggtacga ataccaatct
     4861 gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac atggtgccgt
     4921 ggtcaaattc acaaccgttc gcgtaggtat cggtgtgggc gtcaaagtgt accagcgcca
     4981 ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag tggtcaccac
     5041 cgaaggagag catacgctta ccggcagcca gcagcttctc tgcgtgtgcc tgcagcttct
     5101 cgctcatctc acgggcatcg ccgaaagcat ataccagatc accacagtcc acgacgttca
     5161 gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc agattcgtcg
     5221 aaacctgacg gatagctgcc ggaccgtggc gtccccccgc acgaccagaa gtggccatat
     5281 cgaacggcac accagtaatc acccagtcag catcgctgtc atacggctgg aagttcatcg
     5341 gcaggcgtaa aaaaccaaag gcgttggaaa ccagtgagtt atcgtattga tgacctaagg
     5401 tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg cgtcgttagg
     5461 cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc gccttaaatg
     5521 ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag ccattgttat
     5581 ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgcctgg ccttgtaatt
     5641 caaaggcggc aaagcctatt acaccggtat tacgcacatc gccttgcgcc gaattggctg
     5701 cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc ggtatgttta tctttgcgga
     5761 atcctttaat cgctaacgaa tctccggcat tcacaatata gccgttgtta ttcagcgaac
     5821 cttgcttgcc gttcagcaca tccaggccat caaccgtagc aacgatctcg tagttggtgt
     5881 tacgactgta attacggaca tataattgat aattcattcc gactctggca tggacaatat
     5941 agcgtggttc attattggat gaatcataaa cacgcgtaat aggtagagaa ttaaaattac
     6001 catcgcgaat agcatattcc agttcgttaa tacgaatgct ataaacgcgg tcgtagccgg
     6061 aaggaacatt agtcgaataa ctaataacga tcatctccgc cggatcgcgc caggctcgct
     6121 cagcagatac gccctgaacc gttgagtgca catccccacc ccaggcggtg ccgacattgc
     6181 ccgatgcact gctattatac tgcgcattac tggaacaacc ggccagcagc acgattaatg
     6241 ccaccaacgc gctacgtact ttccattttt tcataaccgt tccctctgca tttaattaat
     6301 gcagcgattg tagggagatt atttaataag aaaagcagct aaattatttt taagaatata
     6361 taaaaagaaa gcggaccgat ttaagtaaat aaatcgaccc gccagcagaa taatcttaat
     6421 tattcatctt caagataggt ataaccgtac aaacctgcct cgaactcttc aaggaactgt
     6481 tgttgcagtt cagcatcaag atcggttttc ttcacttgat cacggaactg agttaacagc
     6541 gttttcggat cgagctgtac atattgcagc atgtccgcca cggtatcacc ttcgtcagac
     6601 agttccactt cgacactacc gtcagggaac acgaacacgt caaccgcttc ggtatcaccg
     6661 aacaggttgt gcatgttgcc gaggatctcc tgatatgcgc cgaccataaa gaaaccgagc
     6721 atcggcggat tctctggatc gtactccggc attggcattg tcgtggcaat accgtcacca
     6781 tcaatatagt ggtcgatagc accgtcggag tcacaggtaa tatccagcag cacagcgcgg
     6841 cgttccggca cttgatctaa cccttccagc ggcagaaccg ggaacaactg gtcgatcccc
     6901 catgcgtccg gcattgactg gaacagcgag aagttgacgt acattttgtc cgccatacgt
     6961 tcctgcagct cgtcgataat cggacgatga gcacggtttt gcggatccag ctgcttttgc
     7021 acttcatggc acatgctcaa ataaagctgc tcagcccatg cacgttcttg caggctaaag
     7081 gtgccggaag agtagccgat atgaatgtcg tgcagatcca tctgactgtc gtgtaaccat
     7141 tcacgcagag aacggcgagt tcccggttcg tgcatctcct gccaggtttc ccacatgctt
     7201 tgcagcgcgc gcggcgcatc ttctgcaggc gcggtcggca ccgtgtattc gttacgttcc
     7261 acgccgatga tattagacac cagcacggtg tgatgcgcag tcactgcacg acccgattca
     7321 gtgattaccg tcggatgcgg cagaccgttt tcttcacacg catcaccaat cgcccagatg
     7381 atgttgttgg catattcatt gaggccgtag tttaccgaac agtcagactg cgaacgggtg
     7441 ccttcatagt ccacgcccag accgccgccg acgtcgaagc actgaatatt gacgcccagc
     7501 ttgtgcagtt ccacatagaa acgcgcggat tcacgaacgc ccgtggcgat atcgcgaata
     7561 ttcgccatct gcgaaccgag gtggaagtgc agtagttgca ggctgtcgag acgctcagct
     7621 tcacgcagcg tttcaaccag ttgcagtacc tgagtcgcag ccaggccgaa cttcgatttt
     7681 tccccgccgg aagactgcca tttacccgag ccctgcgaag ccagacgtgc acgcacgccc
     7741 agacgaggaa cgacattcag acgttctgct tcatccagca caatggcgat ttctgacatc
     7801 ttctcaatga ccagatagac cttgtgcccc atcttctcgc caattaatgc caggcggata
     7861 tattcgcggt ctttataacc gttgcagacg atgacgctac gggtcatgcc agcatgtgcc
     7921 agcactgcca tcaactcggc tttggaaccg gcttccagac ccagcggttc gcccgaatga
     7981 atcagggact caatcacgcg acggtgctgg ttaactttga tcggataaac aaggaagtaa
     8041 tcgccgttat agccgtagga ttcccgcgca cgtttgaacg cggcgttaat ggaacgcaaa
     8101 cggtgctgca ggatctgtgg gaaacagaac agtgcaggca gacgctggcc ctgtgcttca
     8161 cgagttttca ctaactgcgc gagatcgacg cgagcttccg ggacgtccgg gtccgggcac
     8221 acgctgatgt ggcccagctc gttaacgtca tagtagttat tgccccacca ggcaatattg
     8281 taagtacgca gcatcttgct ggcttcctgg gagctcattg caacctcctg catggagcgt
     8341 agtacaccgt gttcgcccgc tgacgaaggc aaacccatag acatgtcgtc agacatagcg
     8401 aacctcaaat tattttatta agtgtaaaac agttaacgac tatcgcagcc tggaactgcg
     8461 ataacaaccc ataagcacat ggattttcca gcagtgaatg ctgacgctcc aactgcgcaa
     8521 ccggtttctt tttcataaca ttattaagca cataaccgaa cgtaagtgtg acagttcggc
     8581 gaaaccacga gaaaactctt gtttttacaa gagcgccctt gttcagtcct cagtaactgt
     8641 aaccagctct tgaatcctga gaagcgccga gatgggtata acatcggcag gtatgcaaag
     8701 cagagatgca gagtgcgggg aacgaatctt caccagaacg gtgagacagg ttaagcagca
     8761 gacaacggtt cattatttcg tatcacctcc acggccgcct gttaagacga acccacaagc
     8821 caaaactctg atttcaaccc ggctggaagt ggcaacacga aagaaacgtc gtgtgctttt
     8881 tatttaagcc gcgcgccgcg ttttataccc cacaatggca gaaaattgca aaagataaat
     8941 acgcagaatg ccggcattgt caggaaaaat ttccagccac gtttttaaca gaatgagaca
     9001 cgattcaaaa aaaagtggaa atcgggtgaa gaattgacct aaaatagcca tccagatgtt
     9061 aatccatcca taccgattaa cactcagact gccagtgttt ttaacctgca gagtcgtggt
     9121 aggatccgct accacagaaa atccacacaa cagtttgagc taaccaaatt ctctttaggt
     9181 gatattaaat atggcaaaac acctttttac gtccgagtcc gtctctgaag ggcatcctga
     9241 caaaattgct gaccaaatct ctgatgccgt tttagacgcg atcctcgaac aggatccgaa
     9301 agcacgcgtt gcttgcgaaa cctacgtaaa aaccggcatg gttttagttg gcggcgaaat
     9361 caccaccagc gcctgggtag acatcgaaga gatcacccgt aacaccgttc gcgaaattgg
     9421 ctatgtgcat tccgacatgg gctttgacgc taactcctgt gcggttctga gcgctatcgg
     9481 caaacagtct cctgacatca accagggcgt tgaccgtgcc gatccgctgg aacagggcgc
     9541 gggtgaccag ggtctgatgt ttggctacgc aactaatgaa actgacgtgc tgatgccagc
     9601 acctatcacc tatgcacacc gtctggtaca gcgtcaggct gaagtgcgta aaaacggcac
     9661 tctgccgtgg ctgcgcccgg acgcgaaaag ccaggtgact ttccagtatg acgacggcaa
     9721 aatcgttggt atcgatgctg tcgtgctttc cactcagcac tctgaagaga tcgaccagaa
     9781 atcgctgcaa gaagcggtaa tggaagagat catcaagccg attctgcccg ctgaatggct
     9841 gacttctgcc accaaattct tcatcaaccc gaccggtcgt tttgttatcg gtggcccgat
     9901 gggtgactgc ggtctgactg gtcgtaaaat tatcgttgat acctacggcg gcatggcacg
     9961 tcacggtggc ggagcattct ctggtaaaga tccatcaaaa gtggaccgtt ccgctgcgta
    10021 cgcagcacgt tatgttgcaa aaaacatcgt tgctgctggc ctggccgatc gttgtgaaat
    10081 tcaggtttcc tacgcaatcg gcgtggctga accgacttcc atcatggtag aaactttcgg
    10141 tactgagaaa gtgccttctg aacaactgac tctgctggta cgtgagttct tcgacctgcg
    10201 cccatacggt ctgattcaga tgctggatct gctgcacccg atctacaaag aaaccgcagc
    10261 atacggtcac tttggtcgtg aacatttccc gtgggaaaaa accgacaaag cgcagctgct
    10321 gcgcgatgct gccggcctga agtaatcttt cttcacctgc tttcaaaggc cagcctcgcg
    10381 ctggcctttt tcttttacct caccgcggtg gataggcgtt cacgccgcat ccggcaaaaa
    10441 aaccgcccgc acaataacat cattcttcct gatcacgttt caccgcaaat tatcatcaca
    10501 actgaaaccg attacaccaa ccacaacaga caaagatttg taatattttc atattattat
    10561 tcggttttca cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt
    10621 taatctatga tgatataact caattatttt catgcactt
//
LOCUS       DAZUCE010000179        10588 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZUCE010000179 DAZUCE010000000
VERSION     DAZUCE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.179"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(315..464)
                     /gene="ydfW"
                     /locus_tag="ACHY03_003811"
     CDS             complement(315..464)
                     /gene="ydfW"
                     /locus_tag="ACHY03_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFS3906991.1"
                     /translation="MDNARIDLRSKYYVKSKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKID"
     gene            complement(537..1655)
                     /locus_tag="ACHY03_003812"
                     /pseudo
     CDS             complement(537..1655)
                     /locus_tag="ACHY03_003812"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
     gene            complement(1689..2108)
                     /locus_tag="ACHY03_003813"
     CDS             complement(1689..2108)
                     /locus_tag="ACHY03_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFS3906992.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKARRSRLLLTPSCNVPFEPF"
     gene            complement(2149..3114)
                     /locus_tag="ACHY03_003814"
     CDS             complement(2149..3114)
                     /locus_tag="ACHY03_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3906993.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTAEQDFLTRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDQFWV
                     KNIMSPSKLREKWDELVIRLGRSPVKRCVNHISEPDTEIPPGFRG"
     gene            complement(3095..3616)
                     /locus_tag="ACHY03_003815"
     CDS             complement(3095..3616)
                     /locus_tag="ACHY03_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS3906994.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(3600..3827)
                     /locus_tag="ACHY03_003816"
     CDS             complement(3600..3827)
                     /locus_tag="ACHY03_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFS3906995.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            3905..4312
                     /locus_tag="ACHY03_003817"
     CDS             3905..4312
                     /locus_tag="ACHY03_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3906996.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            4505..4660
                     /gene="ydfA"
                     /locus_tag="ACHY03_003818"
     CDS             4505..4660
                     /gene="ydfA"
                     /locus_tag="ACHY03_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFS3906997.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            4662..4763
                     /gene="ydfB"
                     /locus_tag="ACHY03_003819"
                     /pseudo
     CDS             4662..4763
                     /gene="ydfB"
                     /locus_tag="ACHY03_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            5724..5912
                     /gene="dicB"
                     /locus_tag="ACHY03_003820"
                     /gene_synonym="ftsT"
     CDS             5724..5912
                     /gene="dicB"
                     /locus_tag="ACHY03_003820"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFS3906998.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            5909..6100
                     /gene="ydfD"
                     /locus_tag="ACHY03_003821"
     CDS             5909..6100
                     /gene="ydfD"
                     /locus_tag="ACHY03_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFS3906999.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            6194..8665
                     /locus_tag="ACHY03_003822"
     CDS             6194..8665
                     /locus_tag="ACHY03_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFS3907000.1"
                     /translation="MSKVFICAAIPGEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPIALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAHQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEKMEGAEHPHKENAGGNQHHDSDNETGETADHS
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            8738..8989
                     /locus_tag="ACHY03_003823"
     CDS             8738..8989
                     /locus_tag="ACHY03_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFS3907001.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            9024..10304
                     /locus_tag="ACHY03_003824"
     CDS             9024..10304
                     /locus_tag="ACHY03_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3907002.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(10492..>10588)
                     /locus_tag="ACHY03_003825"
     CDS             complement(10492..>10588)
                     /locus_tag="ACHY03_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907003.1"
                     /translation="HTFDFQHVADAISLFEQDQKHCCKVLLTFSE"
BASE COUNT         3024 a         2505 c         2377 g         2682 t
ORIGIN      
        1 cccaattagt ctatttaaat cgccacgagt ttaactgaca acccatgatc aattatgaat
       61 tgcaactatt tctgtagtca cttttgtggg gacagtccac aaaactgcca acttccgctt
      121 cttgctctta gcggacatta gcataggcta tttaccataa cgcctcatta cgcgcaccgc
      181 ccagactgac tcagcgcgtt tctggcatat ccccggtaaa acaagtaaca aaccacccga
      241 aaatgaacac cagaaacgcg acttaagaat ctaccctatg aatggatatg cactcaaccg
      301 aatcgatctt ggtttcaatc tattttatcg ggatcaggct tctttttagg taacttcggg
      361 ggcttaactt gctgatgact ttgcgttcgg cgcgtaagcc agggatggtc agctttagat
      421 ttaacatagt attttgagcg taaatcaata cgggcattat ccactcgttc atggacactc
      481 ttttcatcat ccagtggtat aggctccggg ccatcaacat acttttccca gcccagcgct
      541 ttcccgtcat acagaacgtt aatttcaccg tcaaagttct cacatacagt aacaaccgaa
      601 tgcctaagtc gatatccccg gccctcactg cgtacctgaa acgcactgct tttgtactgg
      661 aaagtgagat ttttagacag aacgcgcttc gcctgtaggc tgaagatata acccagttcc
      721 tcttcagaat ggtgcacatc aagatgagca ttatcagtag tatgaggcga cgtagcgaac
      781 cggttgttat aggcttcaat aaaggtcggc aaccatgcat ttgctgtttc aatatcactg
      841 atattctgaa gccgcatttc tttgaccagc ctgtcctgta gtgtctgatt ggcgcgctct
      901 acccgccctt ttgcctgcgg gctgttggca tggattggct cgatgcccag tgtctttatc
      961 gcacgagtga actgggtcag ctcaccttcc cgctctgggt tatttaccct gaatatactg
     1021 tgtctatcag agtagagagc gagcggtacg ccatgatcat taaggtaacc ccggagggtt
     1081 tccatgtaag cccgggttgt ttcagcaggc acaaaacgca acgccatcaa cgcactggtg
     1141 gcatcatcaa tgaaaacgat cagtgtacat ctggggcctc gattttcaaa ccagtcatga
     1201 ggtgagccat caatctggat cagttcaccg taagatggtc gtcgcatgcg gcgttgatat
     1261 atacgggcaa ttttacgacg gcgttcacgc cataacccct cttctatcat ccactttctg
     1321 agagtttcaa cggataaaga taaaccgtgt atctcgcgca atttttcgca cgcaaacgta
     1381 ggtccaaaat cagcgtaacg gcctttgagg agtgaaatta ctgttgctct gaattcagta
     1441 gaaaaggaat tattaggacg ctttccacgt cggtgggaaa ccagaccaga aggcccttca
     1501 tttctatacc gttgcaccag acgtttaacc tgccgaatag aaatgccaat gcgtgccgca
     1561 gcttgttcct gagtaatatg tcgattaatt gactcctgaa taatctgaag tcggtggagt
     1621 tccttatgac tcatcgtaac agtctctttg atcatgaaaa atcccccaga gaattgtctg
     1681 gggacatttt agaatggttc aaaggggaca ttacagcttg gtgttaacaa tagcctgctt
     1741 ctccttgcct tccggcactc gcgacagatt atgtttagac gactgtcgta gcgacgtatt
     1801 tctccgtcag gtaatgacca gataaggtcc ggatcaacca cagcaggttt cttcaccttt
     1861 gccctcgaga gttttttgcg ggcgttttgc cagtccttac gcgcctgttc agacgggaat
     1921 aacccgtaac cagagctgta tacatcgcca ctggcaacca gctctctggc cagaacgctc
     1981 atcagatatc ttgttgcccc agttttagct tccagttgtc gtaacgtctc gcgcccactc
     2041 tggcgtacga gttccaccac ctgcccttta attttttccc gctcttcttg tgtaaaaact
     2101 tttgccacaa gtcctcctga acattacttt ataccctaaa atcaacactt atcccctgaa
     2161 accaggcgga atttctgtat ccggttcaga aatatgattc acacaacgct ttacaggtga
     2221 acgtcccagg cggatgacca gttcgtccca tttttcgcgg agttttgacg ggctcatgat
     2281 gttttttacc cagaattgat ctcgctgtac ccgaccaaac atttcgcaaa tttgtctgtg
     2341 gcttctgcca tccagcatcc gcattgtgcg cacgtcattg gcccaggctg tccagttagg
     2401 ctcttttggt cgcatgatct ctccatcgtc gctggcagcc tgttcgtaaa ggttcacgat
     2461 ccgcccccaa atccactgcg cacacgccaa atcctccctg ctaccccact ggcgtttttt
     2521 cgcactaaac acaaccgcgt cggggttccg ggttaaaaaa tcctgttcag cggtctgcgg
     2581 gtccggttgc gaagcttccg gacgaaaagt gtttttattc tctgtagtaa tctctgttgt
     2641 attctctgta agatcatcag gccattttga cccgatgaca ttgggtcgtt ttgaaccaat
     2701 ggagcgtttc attttgacct cttccatcgt gtcattttga cctgatggag cggcgcattt
     2761 taaaccgatg gattcgctca atttgccacc atctaaaagc tcgctcccgt agttgatcgt
     2821 gtagaaattg gtcatatcgc gctttgattt attgagcttt tcacaacgca aaagccccag
     2881 cgttttcaga cttgcaaacg cgcgctttaa cgttgactct gaccagaatg ggaactgttc
     2941 cagccattgt tccgttgtgt tgtaaatcca gcgaacacca tcacattcca tgccggaatt
     3001 ggtatctctc aaccagtaat gcaactgctg caacacaatg gcttcattta agccaatctt
     3061 catcgcaagc tgtgtgttta taaccagtgg gcgttcagca aaaagaaggc tcataattcc
     3121 atccagcttt ttgttggtat tgctgtcgat acgcaagttt gaaagcaatt gctttttcta
     3181 taagttcgtc agtttcacga tccactacgg caggatcagc aaaaagcagt ccggactcca
     3241 ccacatcgcc atattctttg tttaacccgg cgatcatgta cgtgatgctt tttccgtcac
     3301 taatttcacg atacaacctg aaatcattaa tccgaatagc ctccataatt gcaggcacta
     3361 gcgccgtgaa cttttcacgc ttatccctgg tgtcgatagc cttccagcgt tcgaatatct
     3421 tcactcgatt aacgccaagt gctcgctgat caaccgcgcc accttcatct gtgacacgct
     3481 gaacatcgat gttcgggcgc tctttcaaag cccagaatgc ttcagtgatt aatatcgtcg
     3541 cctgctcctg tgtcattcct ggtcgacata tccaggcatc cagagcctca cgagcctgtt
     3601 caggagtgat tttcattgtt caaccgcccc gcccgcttcg tcttacgata ttcgtcataa
     3661 accttgggat catacagaag ctcgccgcca gatgcctcct gtagacgcat cgcgcgacct
     3721 tcaggaacca gctcttgcca ttgagaaaca gcagatgggt caacgccagc agctttcgct
     3781 actttggctt tcgtaccgta aaaattaatt acgtctgatt taaacatcgc acctccaata
     3841 ttgagttttc tcaatgctaa tcactcaagg aatctcaagt caagggttat taagatatct
     3901 aaatatgaac gagaaaactt taggtcaacg cattagagaa agacgtaagc aggtaggctt
     3961 aagtcaaaac ggtttaagca aagctgctgg cgtatctggc tcatcaattt cattatggga
     4021 aagtgaccat acagccccgc gcgggcaaaa tttgcatcgc ctcgccgagg tattgcaatg
     4081 ttcaccaact tggatactgt ttggcgacga ggacaaaaca ccagctcccc ctgtttcact
     4141 cgatagtgcc ttagacttat cggaagatga gttagagatg ctgcgtttgt atcgcgcact
     4201 tcctaaatca gagcaacaag cacaactcag cgaactccgc gcccgcgttg agaattttaa
     4261 tcgcctgttc accgagctat tagaggctcg caaacgcaac aagcatcaat aatcatccct
     4321 tcacaaaatt ttaaagcctt acatttcaat gtattggctt tatcttgcgc caacacttga
     4381 gttttctcat caaaaaacct tgacgaaaaa taatgagaaa actaaattac ctccatcaag
     4441 acaccgcacg gtgttctcag caaacagttc cgctaccccg gcgttaaggg gaaatgaggt
     4501 cagcatggat actatcgatc ttggcaacag cgaatctctg gtatgtggcg tgttccccaa
     4561 ccaggacggc acgttcaccg cgatgacgta taccaaaagc aaaacgttta aaaccgaaaa
     4621 tggtgcccgt cgctggctgg aaagaaactc aggtgagtga tatggatttc gacacaatca
     4681 tggaaaaggc ttacgaagaa tacttcgaag gccttgccga aggcgaagaa gctctcagct
     4741 tcagtgagtt taaacaggca ctcagaataa gaatgtgctc tcacaatgac gcggagcaca
     4801 aatatgagaa gcaaaatcag accgcagaaa attttgttct ggaacccgga gaaacgcttt
     4861 tcaaaattcc cgttacgtgc cccatttgcg gttttacatc agaagaactt gacgactcct
     4921 gtaacaatca ggaaacaacc aagtatgtcg aagatgatac cgagtgcgca cgaagaacga
     4981 ttatatccac gagtccaaac tccaggacca ataaatctca ctatgagagg gtgattaatc
     5041 cactccccca aaccaataaa aaagatgccg gaggacaaaa aacccaatgg aacaacggga
     5101 tatctgtcaa aaaaagacgt tccattaaag acaaacaacg ctgcgccaac aactgtaaga
     5161 gctttatacc agtaatcaat tttcatgttc ttaagcggat ttattggtgg ttgcgacatt
     5221 gcttaatgaa tccttaaaac tgtggtgatt ttaaggatac cacctcgcct gacgtggtta
     5281 aaagcaggca cacaacacga aagcgcacgg cgaagttcgt ctcactgtac ggtgtcgtta
     5341 aatttaattc gaccgtgcgc ttccggttgt ggcaacccgc aaaatggcgc ggcggtaagt
     5401 atggcggggt tattccttcc ccgttgagga caccgggttg tcaggttgac catacgctta
     5461 agtgacaacc ccgctgcaac gccctctgtt atcaattttc tggtgacgtt tggcggtatc
     5521 agttttactc cgtgactgct ctgccgccct ttttaaagtg aattttgtga tgcggtgaat
     5581 acggctaagc gcacgcggaa cagttaaaac caaaaacagt gttatgggtg gattctctgt
     5641 atccgacgtt aattgttaac tggttaacgt cacctggagg caccaggcac tgcatcacaa
     5701 aattcattgt tgaggacgcg ataatgaaaa cgttattacc aaacgttaat acgtctgaag
     5761 gttgttttga aattggtgtc actatcagta accctgtatt tactgaagat gccattaaca
     5821 agagaaaaca agaacgggag ctattaaata aaatatgcat tgtttcaatg ctggctcgtt
     5881 tacgtctgat gccaaaagga tgtgcacaat gaattcagca tttgcgcttg ttctgacagt
     5941 ttttcttgtt tccggagtgc cagttgatat tgcagtcagt gttcacagga caatgcagga
     6001 gtgtatgact gcagcaaccg aacagaaaat tcccggtaac tgttacccgg tcgataaagt
     6061 tattcaccag gataatatcg aaatcccggc aggtctttaa aacagttccg taataaatat
     6121 ccggtttcat tcttatatgc cagcaatggc agggatttgt tcatccttaa atctgtcatg
     6181 aggttaaaac aaaatgagta aagtctttat ttgcgccgcc attccgggcg aacaggcaat
     6241 aaaggaagaa ggtgccgtcg ctgtagccac tgccattgaa gccggtgatg aacgtcgcgc
     6301 ccgcgcaaaa tttcactggc aattcctgga acattatccg gctgctcagg actgcgctta
     6361 taaatttatt gtctgcgagg ataaacctgg cataccccgc cctgccctcg attcatggga
     6421 tgctgaatat atgcaggaaa accgctggga tgaggagtct gcttcttttg tcccggttga
     6481 gactgaatcc gatccgatga acgtcacttt tgacaagctg gcccctgaag tacagaacgc
     6541 tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt gatatggtta ttagcgcaca
     6601 ggaattgttg caggaagaca tggcaacatt cgacggacat atcgttgaag cgttgatgaa
     6661 aatgccagaa gttaacgcca tgtatccgga gcttaagttg cacgccattg ggtgggttaa
     6721 gcataaatgt attcctggtg ctaaatggcc cgaaattcag gcagagatgc gcatctggaa
     6781 aaaacgtcgc gaaggtgaac gcaaggaaac cggaaaatac acgtctgttg ttgatctcgc
     6841 ccgcgccaga gccaatcaac agtacactga aaattcaaca ggaaaaatca gcccggtcat
     6901 tgctgccatc catcgcgaat acaagcagac atggaaaaca ctggatgacg aactggccta
     6961 cgctctctgg cctggtgatg tggatgccgg aaacattgat ggcagcatcc atcgctgggc
     7021 aaaaaatgaa gttatcgaca acgaccgcga agactggaag cgtatctcgg catcaatgcg
     7081 caaacagcct gatgcccttc gctacgaccg ccaaactatt tttggccttg tccgtgagcg
     7141 tccgatcgac attcacaaag atcccatagc actgaacaaa tatatctgcg aatacctgac
     7201 gacaaagggc gtgtttgaga atgaagaaac agacctgggc actgttgatg ttctccagtc
     7261 atcagaaaca caaactgatg cagtggaaac tgaggtatct gatatcccaa aaaatgaaac
     7321 cgcgccggaa gctgaaccat ctgtagagcg tgaggggccg ttctatttcc tcttcgcaga
     7381 taaggacgga gaaaaatacg gtcgcgcaaa caaactttct ggtctggata aggcactggc
     7441 tgctggcgcc actgaaatca caaaagaaga atattttgcc cgaaaaaatg gcacatacac
     7501 gggcttaccg caaaatgtag ataccgctga agattcagaa caaccagagc cgataaaagt
     7561 taccgctgac gaagtaaaca aaattatgca ggcagccaat atcagccagc ctgacgccga
     7621 taagttgctt gctgcatcac gtggtgaatt tgttgaaggg attagtgacc cgaatgatcc
     7681 gaaatgggtt aaggggatcc agacccgcga ttctgtgaac cagaaccagc atgaatcgga
     7741 acggaactac caaaaagcgg aacaaaacag cccaaatgcg ttacaaaacg agccagaaac
     7801 gaaacagcct gagccagtgg cgcatcagga agtggaaaaa gtctgcaccg cctgcggtca
     7861 gaccggcggt ggcaactgcc ctgattgtgg cgcggtgatg ggcgacgcaa cataccaaga
     7921 aacattcgat gaagagtatc aggttgaagt tcaggaagat gatccggaga aaatggaagg
     7981 cgctgaacat ccacacaagg agaatgctgg cggcaatcag catcacgata gcgataatga
     8041 aactggcgag acggcagatc actcaattaa ggtgaacggt catcacgaaa tcacatccac
     8101 cagcaggacg tgtgaccatc taatgataga ccttgaaacc atgggaaaaa atcctgatgc
     8161 cccgatcatc tcaataggtg caatattttt cgatccgcaa accggagata tgggaccgga
     8221 atttagtaag actatcgatc tggaaactgc tggcggagtc attgatcggg acaccattaa
     8281 atggtggctt aagcaatcac gcgaagcgca atctgccatt atgaccgatg aaatcccgtt
     8341 agatgatgca ctgttacaat tgcgggaatt tatcgacgaa aactccggtg aattttttgt
     8401 tcaggtctgg ggaaatggag ccaacttcga caacacgatt ttgcgccgtt catacgaacg
     8461 gcaggggatc ccctgcccgt ggcgttacta caacgatcgc gatgtacgca caatcgttga
     8521 gctggggaaa gccatagact tcgatgccag aacggctatt ccattcgaag gtgagcgcca
     8581 taatgcactt gatgacgccc gttaccaggc aaaatacgtt tcagttatct ggcaaaaact
     8641 gatcccgagt caggctgatt cttaatgttc aactgtcgcc ggttgtgact ggtattctgc
     8701 aaccggcgct cgtctaatgt aagagataaa gaaatcgatg agcgaagtaa tcatgattgt
     8761 ctctcccggc aaatgggtat ccgaagagca gttaattgcg ctgaaaggaa taaaaaaagg
     8821 tacgttaaaa aaggcccggg aaaaatcgtt tatggaagga agggaatata agcatgtcgc
     8881 tcatgacggt atgccatggg ataacagccc atgcttttac aacctggaag aaattgatcg
     8941 ctggattgag cgccaggcat cagcgagacc aagacgtcat cttacttgac taaaagccac
     9001 actaactaat gagagaagtt gaaatgaaat atccgacagg cgtggaaaac catggaggga
     9061 aattacgtat ctggtttgtt tataaaggcg taagagtcag ggaaaatctg ggggttcctg
     9121 acacagcaaa aaacaggcgc gttgcaggtg aactacgcgc ctctgtttgt tacgcaataa
     9181 aaactggtgt tttcgactat gcaaaacagt ttccctcctc acgcaatctg gaaaaatttg
     9241 gtgaggcccg acaagattta accataaaag aactggctga aaaatttctg gcactgaaag
     9301 aaactgaagt cgcaaaaaca tcactcaaca cataccgtgc cgtcatcaaa aatatcctga
     9361 gcataatcgg tgaaaaaaat cttgcctcat cgattaataa agaaaaatta ctggaggttc
     9421 gtaaagagct actgactgga taccagatcc ccaaaagtaa ctatattgtt acacaaccag
     9481 ggagatcggc tgtaactgta aataattaca tgacaaatct taacgccgtg ttccagtttg
     9541 gtgttgataa cggttacttg gcagataacc cgtttaaggg gatctcgcca ttaaaggaat
     9601 caagaaccat tccggatcct ctttcgcggg aggaattcat ccgtcttatc gatgcgtgca
     9661 gaaatcagca agccaaaaat ttatggtgtg tttctgttta tactggcgtt cgccctggtg
     9721 agctgtgtgc acttggatgg gaggacatag atctgaaaaa tggaacaatg atgatcagga
     9781 gaaatttagc aaaagaccgt ttcacggtac caaaaacaca ggcgggaacc aatcgggtca
     9841 ttcatcttat taagccagca atcgacgctc tccggagtca gatgacatta acgagactga
     9901 gcaaagagca tatcattgat gttcacctca gagagtatgg cagaacagaa aaacaaaaat
     9961 gcacctttgt ttttcaacct gaagtgtcag cgagagtaaa aaattatggt gaccatttta
    10021 ccgttgactc aataaggcag atgtgggacg cagcgataaa acgtgccgga ctccgccatc
    10081 gaaaatcata tcagtcgaga catacttatg cctgctggtc gctgacagct ggtgctaacc
    10141 cggcatttat agcaaaccag atgggccatg cagatgcgca aatggtattt caggtatacg
    10201 gaaaatggat gtctgaaaac aataatgcac aggtagcttt gttaaataca cagttaagcg
    10261 agtttgcccc aaccatgccc cataacgaag caatgaaaaa ttaatttaat atttatcaaa
    10321 tagttaacac gtatgactct tgaaatccat aaattcaagc gcagtgccca gccatcccga
    10381 tactgctgct ttcaccaaat ccttagtgct tctttcgtgt ttttctattg tcataatggt
    10441 tatctctaaa aaagaggtaa gatgcgtact acttactcgc cgttattggt attattcaga
    10501 aaaagtgagt aagactttgc agcaatgttt ttgatcctgt tcaaataaac taatggcatc
    10561 agcaacatgc tggaaatcaa acgtatgg
//
LOCUS       DAZUCE010000180        10553 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZUCE010000180 DAZUCE010000000
VERSION     DAZUCE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.180"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(223..645)
                     /locus_tag="ACHY03_003826"
     CDS             complement(223..645)
                     /locus_tag="ACHY03_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFS3907004.1"
                     /translation="MAKVFTPEEREKIKGQVVELVRQSGRETLRQLEVKTGATRYLMS
                     VLARELVASGDVCNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDRRQNIICRECRKSEVMQRVLAFYRGNFQEVLL"
     gene            complement(661..1422)
                     /locus_tag="ACHY03_003827"
     CDS             complement(661..1422)
                     /locus_tag="ACHY03_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS3907005.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPAQAELPEEITTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQGGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     TAGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQITDSSRHVASEK"
     gene            complement(1445..2191)
                     /gene="ydaV"
                     /locus_tag="ACHY03_003828"
     CDS             complement(1445..2191)
                     /gene="ydaV"
                     /locus_tag="ACHY03_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFS3907006.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIAK"
     gene            complement(2198..3055)
                     /gene="ydaU"
                     /locus_tag="ACHY03_003829"
     CDS             complement(2198..3055)
                     /gene="ydaU"
                     /locus_tag="ACHY03_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFS3907007.1"
                     /translation="MLFVLILSHRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWADVEPSLQEFFCDNGEEWVHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVEVVFEHDVNTKAT
                     NKDTDKDLKTDPPLNPPRGNRGVKKFDPLDIALPNWISVSLWREWVEFRQALRKPIRT
                     EQGANGAIRELEKFRQQGFSPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPD
                     SAIPPGFRG"
     gene            complement(3068..3490)
                     /gene="ydaT"
                     /locus_tag="ACHY03_003830"
     CDS             complement(3068..3490)
                     /gene="ydaT"
                     /locus_tag="ACHY03_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS3907008.1"
                     /translation="MKIKHEHIESVLFALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKTQREKIQKLIPAILAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(3513..3809)
                     /gene="ydaS"
                     /locus_tag="ACHY03_003831"
     CDS             complement(3513..3809)
                     /gene="ydaS"
                     /locus_tag="ACHY03_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaS"
                     /protein_id="HFS3907009.1"
                     /translation="MKKENYSFKQACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATRGEVLCEELRPDIDWSYLRRSACCSQNMSVKQLNDSNKSSFDHT"
     gene            3933..4409
                     /gene="racR"
                     /locus_tag="ACHY03_003832"
                     /gene_synonym="rarC"
     CDS             3933..4409
                     /gene="racR"
                     /locus_tag="ACHY03_003832"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFS3907010.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGSVKSKAEVARHFKVQPPSIYDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPTNSDTKSELLDINNLYQAASD
                     EIRAIVAFLLSGNATEPDWVDHDVRAYIAAMEMKVGKYLKALESERKSQNITKTGT"
     gene            4718..4852
                     /locus_tag="ACHY03_003833"
     CDS             4718..4852
                     /locus_tag="ACHY03_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907011.1"
                     /translation="MVHYEVVQYLMDCCGITYNQAVQALRSNDWDLWQAEVAIRSNKM
                     "
     gene            4863..5018
                     /gene="ydaF"
                     /locus_tag="ACHY03_003834"
     CDS             4863..5018
                     /gene="ydaF"
                     /locus_tag="ACHY03_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaF family protein"
                     /protein_id="HFS3907012.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LEKHTVS"
     gene            complement(5015..5503)
                     /gene="sieB"
                     /locus_tag="ACHY03_003835"
     CDS             complement(5015..5503)
                     /gene="sieB"
                     /locus_tag="ACHY03_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion B family protein"
                     /protein_id="HFS3907013.1"
                     /translation="MLDVFTPLLKLFANEPLEILMYTIIIFGLTLWLIPKEFTVAFNA
                     YTEIPWLFQIIVFAFSFVVAISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFL
                     KTGNLIITSPCRNPVMKKLERKGIIQHQSDSANCSYYLVTEKYSHFMKLFWNSRSRRF
                     NR"
     gene            5945..6166
                     /gene="kilR"
                     /locus_tag="ACHY03_003836"
     CDS             5945..6166
                     /gene="kilR"
                     /locus_tag="ACHY03_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFS3907014.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            6166..6336
                     /gene="ydaE"
                     /locus_tag="ACHY03_003837"
     CDS             6166..6336
                     /gene="ydaE"
                     /locus_tag="ACHY03_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFS3907015.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            6411..6686
                     /gene="racC"
                     /locus_tag="ACHY03_003838"
     CDS             6411..6686
                     /gene="racC"
                     /locus_tag="ACHY03_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HFS3907016.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            6788..9388
                     /gene="recE"
                     /locus_tag="ACHY03_003839"
     CDS             6788..9388
                     /gene="recE"
                     /locus_tag="ACHY03_003839"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFS3907017.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLEPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELWAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGISKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETG
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQQEYHRNLRTLSDCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            9381..10190
                     /gene="recT"
                     /locus_tag="ACHY03_003840"
     CDS             9381..10190
                     /gene="recT"
                     /locus_tag="ACHY03_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFS3907018.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNSDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSLSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            10247..10441
                     /gene="ralR"
                     /locus_tag="ACHY03_003841"
     CDS             10247..10441
                     /gene="ralR"
                     /locus_tag="ACHY03_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFS3907019.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            10434..>10553
                     /gene="rcbA"
                     /locus_tag="ACHY03_003842"
     CDS             10434..>10553
                     /gene="rcbA"
                     /locus_tag="ACHY03_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HFS3907020.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKE"
BASE COUNT         2945 a         2523 c         2407 g         2678 t
ORIGIN      
        1 tcacttaatg ccccatcact aagacttgct atttccaata atcgttcttt agttatttcc
       61 attattatct ccaccgcctt ttcgggcggc ctcctgatga tttgagagtg caggaattcc
      121 tccggttaag gactctattt tattcacagt gctgaattta attattcaga tttggattat
      181 gttttctctt tcacttcacc aagttccgat tattaatttg gctcacaaca gcacctcttg
      241 aaagtttccc cgataaaacg ccagtacacg ctgcataact tcactcttcc ggcactcgcg
      301 acagattatg ttctgacgcc tgtcgtagcg acgtatttct ccgtcaggta atgaccagat
      361 aaggtccgga tcaaccgcag atggtttctt cagctttgcc cttgagagct ttttacgggc
      421 attttgccag tccttacgcg cctgttcaga cgggaataac ccgtaaccag agttgcatac
      481 atcgccgctg gcaaccagct ctcttgcgag aacgctcatc agatatcttg tcgcacctgt
      541 cttgacttcc agttgccgta acgtctcacg cccactctgg cgtacgagtt caacaacctg
      601 tcctttaatt ttttctcgct cttctggtgt aaatactttt gccataggtg cctccggcaa
      661 tcacttttcc gatgcaacat ggcgggaaga atcagtaatc tgtcgtacaa tatccctgtg
      721 cttgttcaac tcacgcagcg cggcgcagac acgctcccac ttctggacat gatttttcgc
      781 ccgacgcagt tcgcggtttg ccatatgcag tgatggtaaa accaggtcat ccgctcgcgt
      841 ttcagtaaac gatggcagcg actgcacaat gtccgccaca gtttctgttt taatatcttc
      901 ctgtgttgca gcctcctgta ctggtaacgc aacacctgcg gtctgaggaa aggccttacc
      961 atcagtttcc gctaccgatg ctgctttcgg ctctgctggt aaattatcgc ccggtatgca
     1021 gtaacgaaat ttaccgccct gatttacgcg aatcagacga cctttgctga ttgccattgc
     1081 cagcgttgaa gccactttgc gtgatgtggt accaaacaat gtagccagct catcagccgt
     1141 ttgtggtcct cgttgttcaa tcgtcgcggt taaatcgcac tctgagattt tcgctactgt
     1201 tgctgtggtg atttcttccg gcagttctgc ctgcgctggc tgttcctgct gaacgttgtt
     1261 atcagccaca cgccaggtgt acgcgctttt atcaacaaaa ccagcctttt tcagttccca
     1321 tagttcgttc agcacttctt cacgactgat atcaagtcgc gcagcaagtt ctatggatgt
     1381 ggcttttccc attgctttca gtgcgtcaaa aacagtctcc attaaatttt tctcccggta
     1441 aaaattactt cgcaattcct ggctggacga cattcggacg ccagctctcc cagttaaaat
     1501 tcacccatcg cccgccgttc atggtcatgc gatccataat ccgctcgccg agcaatgttt
     1561 tcatggcctc atagttcagg tttgtcagca tccccacgct gcgcatcgac gctgtccggc
     1621 gatcaacaat ctggtgcagt accacctgct cgtttttcgt ctcgcgctga atgccaattt
     1681 catcaagaac cagcagatcc acttcgcaca gttcccgcaa aaatttttcg cctgactgcc
     1741 catcgtcata gctggcgtgc agggcactca taacatcagc cacggtaacc acaatcactg
     1801 tctgaccgtc tttcagcagg cgattcccga tagctgccgc taagtggttc ttcccggtac
     1861 caggttttcc gctgaacgca aaatttgtac acccggtcat cagttcatca gcgatggatt
     1921 tcgcctgact caacgcgtat cgctgccctt cgttctgcac ctggtaattc gaaaacgagc
     1981 atttgcggtg caatggctgg atgccagagc gattcagaat tttttccacc cgcaactgac
     2041 gattctgacg attgatctcc tcacaacgtt tctggccttc ggaaagttgc cactcgcgcc
     2101 actccgctac cgtcttgaat ggcgcggtta catgtgacgg ggccagtctg cggatacgtt
     2161 caagaacatc gcctgtcgca atatttttca tggtcagtta ccccctgaag cctggcggga
     2221 tcgcactatc cggtaacgag acggtgttaa cctgtcggag taacgtctca ggtcgaacac
     2281 ctttcggcgc gaacaagccc tggtattcat tggcgatgct gtgtcgaatc acctgctcag
     2341 gtgaaaaacc ctgctggcgg aatttttcca gctcccgtat cgccccgtta gcgccctgct
     2401 ccgttcgaat cggttttcgc aatgcctggc gaaattcaac ccactcacgc caaagcgaga
     2461 cagaaatcca gttcggcaaa gcaatatcca gagggtcaaa ctttttgaca cctcgattcc
     2521 cccggggggg atttaggggg ggatctgttt ttagatcttt atctgtatct ttattagttg
     2581 cctttgtgtt gacatcatgt tcaaacacca cttcaacatc tgtttgaaca cctgttaaat
     2641 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
     2701 cttttcctgc ggctgatttt ttggttaatt tttccctgac tgatgccaga tcttcctcaa
     2761 tccgaagatg cacccattcc tcgccgttat cgcaaaaaaa ctcctgcaag gatggttcaa
     2821 catcagccca tcgctcgtta gtcagacggg caatttttgc cagcctgttt ttaggtattg
     2881 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcaccatgc tcctctgctg
     2941 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
     3001 ccataattgc cccgtatgat gctgcccggt ggcttagaat aagcacaaac agcatggaaa
     3061 cttttgctta atgaacaatg acagaatcgt cggaagaacc gccgccgctg aaatgcgctt
     3121 tccggtaaac ggcttggact gcatcatcat gcgcatcaat tgccgtactt aacgcttcct
     3181 gcgccgccag taatgcacgg cgttccaggg tatcgaagat gcagagtcgg tgacgcagct
     3241 cgcgcggaag gattgccaga attgctggga tcagcttctg aattttttct ctttgcgttt
     3301 tcgtttcacc tttcaaccaa cggtgataga tattctgctg attgttccag tccttgcctg
     3361 gaaccagggg caattcgccg cccccctggc gcagatattc ttcagtaatt gcattggcta
     3421 cccatgcctg ccctttttcg gctgctaggg caaacaacac tgattcgatg tgctcatgct
     3481 tgattttcat gaatcatttg cctcttgatg tttcaggtat gatcaaatga ggatttgtta
     3541 ctgtcattta gttgcttcac tgacatattc tgcgaacaac atgccgaacg tcgtaaatat
     3601 gaccagtcaa tatcaggacg aagttcttcg cacagaacct cacctcttgt tgcacgttca
     3661 attgctggac atctctcggc aggcaattga cgtacccctt tgatccattg atttacgctt
     3721 ggaggtgata cacctaaaag cctagccatt gctgattgcc caccgacaac agcacaagct
     3781 tgcttgaatg aatagttctc ttttttcatc gaatgaactc caaaaacaca cagaaatatt
     3841 aggcgacgcc taacgcaatt gtcaataggc tgtgcctaat gcggtaagga tagggattgc
     3901 ctaatgtaat gcgcatagga gaatattaag caatgcttag tggtaaagac ttaggccgag
     3961 cgatagagca ggccattaac aaaaaaatcg catcgggatc cgtcaaatca aaggcggagg
     4021 tcgcacgcca cttcaaagtc caaccaccat caatttatga ctggattaag aaaggctcta
     4081 taagtaaaga taaacttcca gaattatggc gtttcttttc tgatgttgtt ggtccagagc
     4141 attgggggct taacgaatac cccataccaa cccccaccaa ttcagataca aaaagtgaac
     4201 ttttagatat aaacaacctt tatcaagcag cctctgatga aataagagcg attgtagctt
     4261 tcctgttatc tggaaatgct acagaaccag attgggttga ccacgatgtt cgcgcctaca
     4321 tagcagcgat ggaaatgaaa gtgggtaagt atctgaaagc tctagaatct gaacggaaaa
     4381 gccagaacat cacaaaaact ggaacttaaa cttatatggt ctgacggaaa actcctggat
     4441 tccgttattt aaccccccca tcactttctg ctgtcgccat cacctattag gttacgctca
     4501 aaacattagg catagcctat tgacaatcaa ttaggcatta cctatagttc cagcatacca
     4561 cccaccccgc cccacagaac gccgggcaat acttcgagtt accaggcagt ggtaaggggt
     4621 taagtagcca gcccgaggcg tatgaacatg acggcgggat tcaaattttg cagtgcagca
     4681 gttagttccg ccacccggcg ttaaggggag agataagatg gtgcattacg aagtagttca
     4741 gtatttgatg gattgttgcg gtatcactta caaccaggct gtgcaggctt tacgcagcaa
     4801 cgactgggat ctctggcagg cagaagtcgc tatacgtagc aacaagatgt gagattcgca
     4861 aaatgcaaaa aatcgacctc ggcaacaacg aatccctggt gtgcggcgtg ttccccaacc
     4921 aggatggaac gttcactgcc atgacgtata ccaaaagcaa aacatttaaa accgaaactg
     4981 gtgcgcgccg atggttggag aagcacacag taagctaacg attaaaacgt ctactcctgc
     5041 tgttccagaa taacttcata aaatgggagt atttttcggt gacgagataa taagaacagt
     5101 ttgcgctatc actctgatgt tgaatgatgc ccttccgttc taattttttc ataaccgggt
     5161 tacggcaagg agaagtgata ataagatttc ctgttttaag gaaatcttta aatacagcga
     5221 tttctttctc agataaacga agcaatactc gttgctctgg tagtaatgaa taatgctttt
     5281 gaatatgtgc tcgcaatctt gagaaggaaa tggcgaccac gaaagaaaag gcaaaaacga
     5341 taatctgaaa gagccaaggt atttcagtat aagcattgaa tgcgacagta aactctttcg
     5401 gtatcagcca gagagtgaga ccaaaaatga taatcgtata cataagtatt tcgagtggct
     5461 cgttagcaaa aagtttcaac aatggagtaa atacatccaa catatcaata actctcaact
     5521 gtaagggtat tgaaatgtta acacaagctc tcgctgtagg ggtatagccg agaccaccga
     5581 agcccggagg tggtgaaata aaaccgggca caacacgaag gcgcatttcc gatatccata
     5641 aagagtcggt cttgtctgtt aaatttaaat ggtgggagtg cgcctccggt tgtaaataac
     5701 gacattgctg tgtgtagtcc tggcggcatc agtttttttc ttgaagttcg gctgatgtcc
     5761 gcccttttta aagtgaattt tgtgatgcgg tgaatgcggc taagcgcacg tggcacagtt
     5821 aaaagtcatg ttagtcctta ttggtttggg tgggaaagcc gactgtaatt gttaactggt
     5881 tgcagtcacc tggaggcacc aggcaccgca tcaacaaagt tcatttgtaa aaatggagat
     5941 aattatgatt gcacatcact tcggaactga tgaaatacca cgtcagtgtg tgactcctgg
     6001 cgattatgtt cttcatgaag gccggacata tattgcctcg gcaaacaata ttaaaaagcg
     6061 aaaactatat attcgtaacc tgaccacaaa aacatgcatt actgaccgca tgattaaagt
     6121 cttcctcggt cgtgatggtt tacctgtaaa ggcggagtca tggtgatgac taagaaaata
     6181 aaatgtgctt accacctttg caaaaaagac gttgaagaaa gcaaagctat tgaaagaatg
     6241 cttcacttca tgcacgggat tttatcaaaa gacgaaccga gaaaatattg cagtgaagct
     6301 tgtgccgaaa aagaccagat ggcacatgaa ctttaattaa ttgactattc gaaactgaat
     6361 ttatgccaga aatggcaggt attcgctcaa ccttaattaa ggagaaaaac atgattacca
     6421 attatgaagc cactgttgta actaccgatg acattgttca cgaggtgaat ctggaaggaa
     6481 agcgcattgg ctacgtaatt aaaacagaaa ataaagaaac cccattcact gtggttgata
     6541 tcgatggtcc atcaggcaac gtaaaaacac ttgatgaagg tgtcaaaaaa atgtgcctgg
     6601 tgcatatcgg aaagaatctg cccgcagaaa aaaaagccga atttctggca actctaattg
     6661 caatgaaatt aaaaggtgaa atctgaaaga aatagcctgc gtatggcgca ggctatgaac
     6721 agtgtgtatc cggcaagatc attcactgaa caaaacgaat tttaatctga gttgaggtta
     6781 aaaaacaatg agcacaaaac cactcttcct gttacggaaa gcgaaaaaat catccggtga
     6841 acctgacgtc gtcctgtggg caagcaacga ttttgaatcg acctgtgcca ctctggacta
     6901 cctgatcgtt aagtcaggta aaaaactgag cagctatttt aaagctgttg ccacgaattt
     6961 tcctgtcgtt aatgacctgc ccgctgaagg tgagatcgat tttacctgga gtgaacgcta
     7021 tcaactcagc aaagactcca tgacatggga actaaaaccg ggagcagcac cagacaacgc
     7081 tcactatcaa ggcaatacca acgtcaacgg cgaagacatg actgagattg aggagaatat
     7141 gctactccca atttctggcc aggaactgcc cattcgttgg cttgctcaac acggcagcga
     7201 aaaaccggta acgcacgttt cacgcgacgg actccaggca ttacacattg ctcgggctga
     7261 agaactaccg gctgttactg ccctggctgt ttcccacaaa accagcctgc tcgaaccgct
     7321 ggaaattcgc gaactccaca aactggttcg tgacactgac aaagttttcc ctaatcctgg
     7381 taattcaaac ctgggactga taactgcttt tttcgaagca tacctgaacg ctgactacac
     7441 cgatcgagga ctgctgacaa aagagtggat gaagggtaat cgtgtttcac acatcactcg
     7501 cacggcttcc ggtgctaatg ctggcggcgg aaacctcacc gatcgcggcg aaggtttcgt
     7561 acacgatctg acgtcactgg cgcgcgacgt agccactggc gtactggccc gttcaatgga
     7621 tctggacatc tataaccttc atccggcaca cgctaaacgc attgaggaaa ttatcgctga
     7681 aaataaaccg cccttttctg ttttccgcga caaattcatc accatgcctg gcgggctgga
     7741 ttattcccgc gccatcgtgg ttgcgtccgt aaaagaagca ccaattggga tcgaggtcat
     7801 ccccgcgcac gtcactgaat atctgaacaa agtactgact gaaaccgatc atgccaaccc
     7861 tgatccggaa atcgtggata ttgcctgcgg tcgctcctct gccccgatgc cgcagcgagt
     7921 aacagaagaa ggaaaacagg atgatgaaga aaaaccgcaa ccatctggaa caacggcagt
     7981 tgaacaggga gaggctgaaa caatggaacc ggacgcaact gaacatcatc aggacacgca
     8041 gccgctggat gctcagtcac aggtaaattc tgttgatgcg aaatatcagg aactgtgggc
     8101 agaactccat gaagcccgga aaaacattcc atcaaaaaat cctgtcgatg ccgataaatt
     8161 gcttgctgca tcacgtggtg aatttgttga cggaattagc gacccgaacg atccgaaatg
     8221 ggtaaagggg atccagactc gcgattgtgt gtaccagaac cagccagaaa cggaaaaaac
     8281 cagcccggat atgaatcaac ctgagccagt agtgcaacag gaaccggaaa tagcctgcaa
     8341 tgcctgcggc cagactggcg gggataactg ccctgactgt ggtgcggtga tgggcgacgc
     8401 aacataccag gaaacattcg atgaagagag tcaggttgaa gctaaggaaa atgatccgga
     8461 ggaaatggaa ggcgctgaac atccgcacaa tgagaatgct ggcagcgatc cgcatcgcga
     8521 ttgcagtgat gaaactggcg aagtcgcaga tcccgtaatc gtagaagaca tagagccagg
     8581 tatttattac ggaatttcga atgagaatta ccacgcgggt cccggtatca gtaagtctca
     8641 gctcgatgac attgctgata ctccggcact atatttgtgg cgtaaaaatg cccccgtgga
     8701 caccacaaag acaaaaacgc tcgatttagg aaccgctttc cactgccggg tacttgagcc
     8761 ggaagaattc agtaaccgct ttatcgtagc acctgaattt aaccgccgga caaactccgg
     8821 aaaagaagaa gagaaagcgt ttctgaggga atgcgcaagc acaggaaaaa cggttatcac
     8881 tgccgaagaa ggccggaaaa ttgaactcat gtatcagagc gttatggctt tgccgctggg
     8941 gcaatggctt gttgaaagcg ccggacacgc tgaatcatca atttactggg aagatcctga
     9001 aacaggaatt ttgtgtcggt gccgtccgga caaaattatc cctgaatttc actggatcat
     9061 ggacgtgaaa actacggcgg atattcaacg attcaaaacc gcttattacg actaccgcta
     9121 tcacgttcag gatgcattct acagtgacgg ttatgaagca cagtttggag tgcagccaac
     9181 tttcgttttt ctggttgcca gcacaactat tgaatgcgga cgttatccgg ttgaaatttt
     9241 catgatgggc gaagaagcaa aactggcagg tcaacaggaa tatcaccgca atctgcgaac
     9301 cctgtctgac tgcctgaata ccgatgaatg gccagctatt aagacattat cactgccccg
     9361 ctgggctaag gaatatgcaa atgactaagc aaccaccaat cgcaaaagcc gatctgcaaa
     9421 aaactcaggg aaaccgtgca ccagcagcag ttaaaaatag cgacgtgatt agttttatta
     9481 accagccatc aatgaaagag caactggcag cagctcttcc acgccatatg acggctgaac
     9541 gtatgatccg tatcgccacc acagaaattc gtaaagttcc ggcgttagga aactgtgaca
     9601 ctatgagttt tgtcagtgcg atcgtacagt gttcacagct cggacttgag ccaggtagcg
     9661 ccctcggtca tgcatattta ctgccttttg gtaataaaaa cgaaaagagc ggtaaaaaga
     9721 acgttcagct aatcattggc tatcgcggca tgattgatct ggctcgccgt tctggtcaaa
     9781 tcgccagcct gtcagcccgt gttgtccgtg aaggtgacga gtttagcttc gaatttggcc
     9841 ttgatgaaaa gttaatacac cgcccgggag aaaacgaaga tgccccggtt acccacgtct
     9901 atgctgtcgc aagactgaaa gacggaggta ctcagtttga agttatgacg cgcaaacaga
     9961 ttgagctggt gcgcagcctg agtaaagctg gtaataacgg gccgtgggta actcactggg
    10021 aagaaatggc aaagaaaacg gctattcgtc gcctgttcaa atatctgccc gtatcaattg
    10081 agatccagcg tgcagtatca atggatgaaa aggaaccact gacaatcgat cctgcagatt
    10141 cctctgtatt aaccggggaa tacagtgtaa tcgataattc agaggaataa ttcagcctgg
    10201 cggtgtaatg caccgccaac ttgaaatatt ttttatgaga aaaattatga gatatgacaa
    10261 tgttaaacca tgtccatttt gtggttgtcc atcagtaacg gtgaaagcca tttcaggata
    10321 ttaccgagcg aagtgtaacg gatgcgaatc ccgaaccggt tatggtggaa gtgaaaaaga
    10381 agcactcgaa agatggaata aacgaaccac tggaaataat aatggaggtg ttcatgtata
    10441 aaattaccgc cactattgaa aaggaaggtg gcactcctac taactggaca agatattcaa
    10501 aatctaaact aacgaaatca gaatgcgaaa aaatgctctc aggtaaaaaa gaa
//
LOCUS       DAZUCE010000181        10545 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZUCE010000181 DAZUCE010000000
VERSION     DAZUCE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.181"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            121..1488
                     /gene="dcuC"
                     /locus_tag="ACHY03_003843"
     CDS             121..1488
                     /gene="dcuC"
                     /locus_tag="ACHY03_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFS3907021.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHKLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1531..2028)
                     /gene="sspB"
                     /locus_tag="ACHY03_003844"
     CDS             complement(1531..2028)
                     /gene="sspB"
                     /locus_tag="ACHY03_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFS3907022.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2034..2672)
                     /gene="sspA"
                     /locus_tag="ACHY03_003845"
     CDS             complement(2034..2672)
                     /gene="sspA"
                     /locus_tag="ACHY03_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFS3907023.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3067..3459)
                     /gene="rpsI"
                     /locus_tag="ACHY03_003846"
     CDS             complement(3067..3459)
                     /gene="rpsI"
                     /locus_tag="ACHY03_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFS3907024.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3475..3903)
                     /gene="rplM"
                     /locus_tag="ACHY03_003847"
     CDS             complement(3475..3903)
                     /gene="rplM"
                     /locus_tag="ACHY03_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFS3907025.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4122..5249)
                     /gene="zapE"
                     /locus_tag="ACHY03_003848"
     CDS             complement(4122..5249)
                     /gene="zapE"
                     /locus_tag="ACHY03_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFS3907026.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5443..5841
                     /gene="zapG"
                     /locus_tag="ACHY03_003849"
     CDS             5443..5841
                     /gene="zapG"
                     /locus_tag="ACHY03_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFS3907027.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5995..7362
                     /gene="degQ"
                     /locus_tag="ACHY03_003850"
     CDS             5995..7362
                     /gene="degQ"
                     /locus_tag="ACHY03_003850"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFS3907028.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7452..8519
                     /gene="degS"
                     /locus_tag="ACHY03_003851"
     CDS             7452..8519
                     /gene="degS"
                     /locus_tag="ACHY03_003851"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFS3907029.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8582..9520)
                     /gene="mdh"
                     /locus_tag="ACHY03_003852"
     CDS             complement(8582..9520)
                     /gene="mdh"
                     /locus_tag="ACHY03_003852"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFS3907030.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9955..10425
                     /gene="argR"
                     /locus_tag="ACHY03_003853"
     CDS             9955..10425
                     /gene="argR"
                     /locus_tag="ACHY03_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFS3907031.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2584 a         2687 c         2637 g         2637 t
ORIGIN      
        1 tcacctacaa ttaaaaacac gacatcagca ccataaatag acttgcaaaa aatataacat
       61 cattgttttc aatctgccat ttatgggatt gaccgttttc ttttgacacg gagttcaaca
      121 atgttcggca taattatatc tgtcatcgta ttaattacga tgggctattt gatcctgaaa
      181 aactacaaac ctcaggtggt gctggctgcc gcaggtatct tcctgatgat gtgcggtgtc
      241 tggttagggt tcggtggtgt actcgatccc accaaaagca gcggctactt gatcgtcgat
      301 atttataatg aaatcctgcg catgctgtcc aaccgcattg ccggattggg gctgtcgatt
      361 atggcggtgg gcggttatgc ccgctacatg gagcgcatag gggccagtcg cgcgatggtg
      421 agcttgttaa gccgcccgtt aaaactcatt cgctcgccgt atattattct gtcggcaact
      481 tacgtcatcg gccaaatcat ggcgcagttt attaccagcg cctccggtct gggtatgttg
      541 ctgatggtca ccttatttcc gacgctggtg agtctgggag taagtcgcct ctctgcggtg
      601 gcggttatcg caaccacgat gtccattgag tgggggattc tggaaacgaa ctccattttt
      661 gcagcccagg tcgcgggaat gaaaattgcc acttacttct tccactacca gcttccggtc
      721 gcctcttgcg tcattatctc ggtggcgatc tcccactttt tcgtgcaacg cgcttttgac
      781 aaaaaagata aaaatatcaa tcacgaacag gcagagcaaa aaactctcga taatgtcccg
      841 ccgctctatt acgccatttt acctgtgatg ccgttaatcc tgatgctcgg ctcgctattc
      901 cttgcccaca tcgggctgat gcagtcagaa ctgcatctgg tggtggtgat gttactgagt
      961 ttgacggtga cgatgtttgt tgagttcttc cgcaagcata aattacgcga aacaatggac
     1021 gatgtgcagg cgttttttga cggcatgggt acgcagtttg ccaacgtggt aacgctggtg
     1081 gtcgcgggtg aaatatttgc gaaaggctta acgacgattg gcactgtcga tgcggttatc
     1141 aggggtgcgg agcattctgg tctgggcggt attggcgtga tgattattat ggcgctggtc
     1201 attgccattt gtgccattgt gatgggctct ggcaatgcgc cgtttatgtc atttgccagt
     1261 cttattccga atatcgcagc cggactacat gtaccagcgg ttgtaatgat tatgccgatg
     1321 cattttgcca cgacgctagc gcgcgcggtt tcgccgatta ctgcggtggt agtcgttacg
     1381 tcaggaattg caggcgtttc gccttttgcg gtggtgaagc ggacagcgat ccccatggca
     1441 gtcggtttcg tggtgaatat gattgccaca atcacgctat tttattaagt cattaaaaga
     1501 caaaacaggc cgccagggcc tgtttttgta ttacttcaca acgcgtaatg ccggtcgacc
     1561 accgcgtggt ggctgcggtg gttcatcgtc aggatgagtg tcatcatcgt gatctggctt
     1621 atcgccatca ataaccgaca taacggtttc gttgtctgcc gatgcctctt catcattcat
     1681 gatgctggca tcttcatcgt aggcagcttc aggctcaaac atcgtgccag cgccattttc
     1741 acgggcgtag atagccagca cggcagccag cggcacagaa acctgacgcg gaatgccacc
     1801 aaagcgcgcg ttaaagcgca cctcatcatt cgccagttcc agattgccga cagcacgcgg
     1861 cgcaatgttg agtacgattt gcccgtcacg cgcatattcc ataggaacct gcacgccagg
     1921 gagcgtcaca tccaccacca ggtgcggcgt gagctggtta tccagcaacc actcatagaa
     1981 tgcacgcagc agatagggac gacgtggtgt tagctgtgac aaatccatac agattaactc
     2041 cggcccagac gcatttcacg ttctgcttca gttaaagaag caaggaaaga gtcacgctca
     2101 aagacgcggg tcatatagcc tttcagctct ttcgcacccg ggccgctgaa ctcgataccc
     2161 agttgcggca gacgccacag cagcggagca aggtagcaat cgaccaggct gaactcatcg
     2221 ctcaggaagt acggcttctg accgaagacc ggcgcaatcg ccagcagttc ttcgcgcagt
     2281 tgcttacgtg cggcatctgc ttcagaagca gaaccgttga tgatggtgtt catcagcgtg
     2341 taccagtctt tttcgatgcg atgcatgtac agacggcttt caccgcgagc taccgggtaa
     2401 acaggcatca gtggcggatg cgggaaacgc tcatccagat attccataat gatgcgagat
     2461 tcccacaggg tcagctcacg atccaccagg gtcggaacgc tctgattcgg gttgaggtca
     2521 atcagatcct gaggcggatt gtccttttcc acgtgttcga tctcgaaact tacacctttc
     2581 tcagccagca caatgcggac ctgatggcta tagatgtcag taggaccgga aaacagcgtc
     2641 attaccgaac gtttgttggc agcgacagcc atgaaaacct ccaggtatag tcagaatttt
     2701 tactgctacc agccaccagg tggccagtca gaagttgtgt tacccagtaa ggaacgactc
     2761 tctttgttcg aaaatcaaac aaaaaatgag caatacccga catttgggca gaaaattgga
     2821 tgatagttta ccagattttg cgacctttgt ggtgagtcga tgccggaaat ggggaaaaag
     2881 agatgcgctt tagtctgaaa tagttgactt agtcccttat tggcgatgtg gtttttgttt
     2941 tgcctgtctg tcagacggca gcaaaaagca actttccatt ttttacgctg attcagattt
     3001 tagccataaa aaaacccgcc gaagcgggtt ttttcgcaaa ttgttttctg ccggagcaga
     3061 agccaattaa cgtttggaga actgcggacg acgacgtgct ttacgcagac cgactttctt
     3121 acgttcaacc tgacgagcgt cacgagtaac gaagccagct ttacgcagtt cagaacgcag
     3181 ggactcgtcg tattccatca gagcgcgggt gataccgtga cggatcgcac cagcctgacc
     3241 agagatacca ccacctttaa cggtgatgta caggtccagt ttctcaacca tgtcgaccag
     3301 ttccagcggc tgacgaacta ccatgcgagc agtttcacga ccgaagtact gttccagaga
     3361 acgttggttg attacgattt taccgttgcc cggtttgatg aaaacgcgag ctgcggaact
     3421 tttgcggcga ccagtgccgt agtattgatt ttcagccatt gcctataatc ccgattagat
     3481 gtcaagaact tgcggttgct gtgccgcgtg gttgtgctcg ttacccgcgt aaactttcag
     3541 tttacggaac atagcacgac ccagcgggcc ttttggcaac atgcctttaa ccgcgatttc
     3601 aatcacacgc tcaggacggc gagcaatcat ctcttcaaag gtcgcttgtt tgataccacc
     3661 gatgtggccg gtgtggtgat agtacacttt gtcagtacgc ttgttgccgg ttacagcaac
     3721 tttgtcagcg ttcagaacga tgatgtaatc accggtatct acgtgcggag tgtattccgc
     3781 tttgtgctta ccgcgcaggc gacgagccag ttcagtagcc agacggccca gagttttacc
     3841 ggtcgcgtca acaacatacc agtcgcgttt tacggtttct ggtttagctg taaaagtttt
     3901 cattaaaagc ttacccaata aatagttaca cgttggtgaa cacccaaacg tcttcaattg
     3961 ttgaggttca cacgacaaag tccggcaaac ctaccccttc gaatagccta tgccagcaca
     4021 caaaaagttt tgggaaaaaa actttcttgt aacgtggggt cgcaggatta tagagaagtc
     4081 ggggtcaaag atcgacccct ttttgtgatt tgtgacaggt tttaacccgc caaatgctcg
     4141 cgcttcagat actcttcgct ttgcatctct tgcagacgtg acaggcaacg ctggaactca
     4201 aacttcagcc gatcgccctg ataaatttca tacaacggca cttctgcact caccactaat
     4261 ttgacatggc gctcgtaaaa ctcatccacc agcgcaataa agcgccgcgc ttcactctcc
     4321 atcaaccgcg tcataactgg tacatcaaac aacatgaccg tatgaaagag acgtgagagc
     4381 gcaatatagt catgctgact gcgggcgtcg acgcacagcg tagtaaaaga gaccgccagc
     4441 gtctggttct cgacgcccat tgtcgctaat ggccgatggt tgatttctaa cgtcggtgaa
     4501 ttttctcgtt tcccccccgc cagcgccaac catagtttat ccatttgcgc ccgggtttca
     4561 tcgtggagtg gcgaaagcca cagatgcgcc tgagtgagtg tacgcagtcg ataatcaaca
     4621 ccagcgtcca cgttcattac atcacaatgc tgtttaatgg catcgattgc aggcagaaaa
     4681 cgcgcacgtt gcaggccatt tcgataaagt tcatccggcg gaatatttga cgtcgctacc
     4741 agggtaatac cgcgggcgaa cagggctttc atcagaccgc caagtagcat ggcgtcggta
     4801 atatcagaaa caaaaaattc gtcaaaacag agcacgtcag tttcggcttt aaagcgatcg
     4861 gcaataattt ccagcggatc ggtctgcccc tgtaaggcag ttagctcttc gtgcacccgc
     4921 agcataaaac ggtgaaagtg caggcgctgt ttccgctctc ccggcaggct ttgatagaaa
     4981 aggtccatca gccaggtttt cccgcgtcct acaccgcccc acatatataa gccacgcact
     5041 ggcgtatgct ttgtgtcttc gcgtttaccc cacagcttac cgacccgcgc cattagccca
     5101 ctcgtcctgg gggctggtgg cgtgctattg atgagttcct gataaataat ttccaggcgg
     5161 ctgacggcct ctttttgaac gtcgtcgggt tgatggctgc cttcattgag cgccttcagg
     5221 tattgcgatg ttggggtaac gctttgcatg atcttattgt tattccttga ataatcggtg
     5281 cgccgttgtt cacggttgac gaaaaaaagg ccgttctaca ctacgcgata tgcagtcggg
     5341 attccacttc tgtggaatta acggttatag tggcataatc agccgcaggc atggagcctg
     5401 aagccaacac cctacggaaa caaaagacaa cgggagatgt tcatgacctg ggaatatgcg
     5461 ctaattgggt tagtcgtcgg catcattatt ggtgctgtgg ccatgcgttt tggtaatcgt
     5521 aaactacgcc agcaacaggc gttgcagtac gaactggaaa agaataaagc tgaactggac
     5581 gagtatcgcg aagagctggt tagccacttt gcccgcagcg cggaattact ggataccatg
     5641 gcgcacgact atcgccagct gtatcagcac atggcgaaaa gctccagcag cctgctgccg
     5701 gaactgtctg ctgaagcaaa cccgttccgt aatcgtctgg cagagtctga agccagcaac
     5761 gatcaggcac cggtgcagat gcctcgcgac tattctgaag gcgcatccgg cctgctgcgt
     5821 actggcgcga agcgcgacta atttattttt cgggcgcagc cattgcgccc tcctcttctc
     5881 tccctccccg actatcattt aatctggtgt ctcattgtta gccgtctgaa aattcaataa
     5941 catcaaactg ttttgaatct cttttcttat cattcaggta cgagagcagg aataatgaaa
     6001 aaacaaaccc agctgttgag tgcattagcg ttaagtgtcg ggttaactct ctcggcgtca
     6061 tttcaggccg tcgcgtcgat tccaggccag gttgccgatc aggcccccct ccccagtctg
     6121 gctccaatgc tggaaaaagt gcttccggca gtggtgagcg tacgggtgga aggaacggcc
     6181 agtcagggac agaaaatccc ggaagaattc aaaaagtttt ttggtgatga tttaccggat
     6241 caacctgcac aacccttcga aggtttaggc tccggtgtca tcatcaacgc cagtaaaggc
     6301 tatgtgctga ccaacaacca tgtgattaat caggcacaga aaatcagtat tcagctcaat
     6361 gatgggcgcg agtttgatgc aaaactgatt ggtagcgatg accagagcga tatcgccctg
     6421 ttgcaaattc aaaacccgag caaattaacg caaatcgcta ttgccgactc cgataaattg
     6481 cgcgtcggtg attttgccgt ggcggtcggt aacccatttg gccttgggca aaccgccacc
     6541 tctggcattg tttccgcatt aggacgcagc gggttgaatc ttgaaggtct ggaaaacttt
     6601 atccagacag atgcttccat taaccgcggt aactccggcg gtgcactgtt aaaccttaac
     6661 ggtgagttaa ttggcatcaa cactgcaatc cttgcgcctg gcggcgggag cgtcggaatt
     6721 ggatttgcca tccccagtaa tatggcgcga acactggcgc agcagcttat cgactttggt
     6781 gaaatcaaac gcggtttgtt aggcatcaaa ggcaccgaga tgagtgccga tatcgccaaa
     6841 gccttcaacc ttgacgtgca gcgtggcgcg tttgtcagcg aagtgttgcc aggttctggc
     6901 tcggcgaaag cgggcgtcaa agcgggcgat attattacca gcctcaacgg caaaccgctg
     6961 aatagctttg ctgagttgcg ctctcgtatc gcgaccaccg agccgggcac gaaagtgaag
     7021 cttggcctgc tgcgtaacgg caaaccactg gaagtagaag tgacgctcga taccagcacc
     7081 tcttcgtcgg ccagcgctga aatgatcacg ccagcgctgg aaggtgcaac gttgagcgat
     7141 ggtcagctaa aagatggcgg caaaggtatt aaaatcgatg aagttgtcaa aggaagccca
     7201 gctgctcagg ctggcttgca aaaagacgat gtgatcattg gcgtcaaccg cgatcgggtg
     7261 aactcgattg ctgaaatgcg taaagtgctg gcggcaaaac cggccatcat cgccctgcaa
     7321 attgtacgcg gcaatgaaag catctatctg ctgatgcgtt aatgtcgtaa accgggcatc
     7381 aggctgacgt gtgatgtccg gttaactcgt ggtatgctgc tgccgttccc ttttttaatg
     7441 acgcctccat catgtttgtg aagctcttac gttccgttgc gattggatta attgtcggcg
     7501 ctattctgct ggttgccatg ccttcgctgc gcagccttaa cccgctttcc actccgcaat
     7561 ttgacagtac cgatgagacg cctgccagtt ataatctggc ggttcgccgc gccgcgccag
     7621 cggtggttaa cgtttacaac cgtggtttga acaccaactc tcacaaccag cttgagatcc
     7681 gcaccctggg atccggtgta atcatggatc aacgcggtta tatcatcacc aataaacacg
     7741 tcatcaacga cgccgatcag atcatcgtcg ccttacagga tggacgtgta tttgaagcat
     7801 tgctggtggg atctgactct ctaaccgatc tggcggtact taaaattaat gccactggcg
     7861 gtttacctac cattccgatc aatgcacgtc gcgtaccgca cattggcgac gtggtactgg
     7921 cgatcggtaa cccgtacaac ctcgggcaga ccattaccca ggggattatt agtgccacgg
     7981 gtcgaatcgg tctgaacccg accgggcggc aaaacttcct acaaaccgat gcttccatta
     8041 accacggtaa ctctggcggc gcgctggtga actcgctggg tgaactgatg ggcattaaca
     8101 cgctgtcgtt tgataagagt aacgatggcg aaacgccgga aggtatcggc tttgcgattc
     8161 ctttccagtt agcaaccaaa attatggata agctgatccg cgatggtcgc gtgatccgcg
     8221 gctacattgg tatcggcgga cgcgagatcg caccactgca cgcgcagggc ggtggtatag
     8281 atcaactgca agggatcgtg gttaatgaag tgtcacctga cggcccggcg gcgaatgcgg
     8341 gtattcaggt caacgatctg attatttcgg tggataacaa accggccatc tctgctctgg
     8401 agacgatgga tcaggtggca gaaattcgcc ctggttcggt gatcccggtt gtagtgatgc
     8461 gtgatgataa gcagttaacg ctgcaggtca ccattcagga atatccggca accaattgag
     8521 tcgtgcgctc aaaacaaaaa accggagtct gtgctccggt tttttattat ccgctaatca
     8581 attacttatt aacgaactct tcgcccaggg cgatatcttt cttgagcgta tcaagcatac
     8641 cttccagcgc gttctgttca aatgcgctca gggtaccgat agatttacgc tcttccacgc
     8701 cgtttttacc cagcagcagc ggttgagaga agaaacgggc gtactgaccg tcgccttcaa
     8761 cgtaggcaca ttcgacaacg ccttgttcgc cctgcagtgc acgaaccaga gacagaccaa
     8821 aacgtgcagc tgcctggccc atagacaggg ttgcagaccc gccaccggct ttcgcttcaa
     8881 ccacctcagt acccgcgttc tggatacgtt tggtcagatc agccacttcc tgctcggtaa
     8941 aactaacgcc aggaacctgt gacagcagcg gcagaatggt aacaccagag tgaccgccaa
     9001 taaccggcac ttcaacttcg cctggctgtt tgcctttcag ttccgcaaca aaggtgttgg
     9061 aacgaatgat atccagcgtg gtaacgccga acagtttgtt tttgtcataa acaccggctt
     9121 ttttcagcac ttcagcagca attgcaactg tggtgttaac cgggttagtg ataataccaa
     9181 tgcacgcttt cgggcaggtt ttcgcaactt gctgtaccag gtttttcacg atgccggcgt
     9241 taacgttaaa caggtcggaa cgatccatac ccggtttacg cgctacacct gcagagataa
     9301 gaacgacatc tgcgccttcc agcgccggag tcgcatcttc accagaaaaa cctttgattt
     9361 tcacagcagt agggatatgg ctcagatcga cggccacacc gggagtcact ggagcgatat
     9421 catacagaga gagttctgaa cctgaaggca gttgggtttt taacagtagt gcaagcgcct
     9481 ggccaatacc gccagcagcg ccgaggactg cgactttcat cctaaactcc ttattatatt
     9541 gataaactaa gatatgttgc tccgctgccg cgaccttaat ccacaaaatt gccgtttaca
     9601 atgaccacat ctcaagaatg tgtagtcacg caagtttagc gtttatgcat ttaattgccg
     9661 taatcaggaa cctaacttac gtaattaaca gtcgcttatg cattagcgca acattccaac
     9721 aggtggtgac aatataccct accgttcagc caaaacaaca tcaatttgat aacaattaat
     9781 ttacttttaa gcagaatttg catgccgtga cgcaggcatg tttctcaata acgaaatttg
     9841 ataaaatccc gctctttcat aacattattt cagccttctt cagggctgac tgtttgcata
     9901 aaaattcatc tgtatgcaca ataatgttgt atcaaccacc atatcgggtg acttatgcga
     9961 agctcggcta agcaagaaga actagttaaa gcatttaaag cattacttaa agaagagaaa
    10021 tttagctccc agggcgaaat cgtcgccgcg ttgcaggagc aaggctttga caatattaat
    10081 cagtctaaag tctcgcggat gttgaccaag tttggtgctg tacgtacacg caatgccaaa
    10141 atggaaatgg tttactgcct gccagctgaa ctgggtgtac caaccacctc cagtccattg
    10201 aagaatctgg tgctggatat cgactacaac gatgcagttg tcgtgattca taccagccct
    10261 ggcgcggcgc agttaattgc tcgcctgctg gactcactgg gcaaagcaga aggtattctg
    10321 ggcaccatcg ctggcgatga caccatcttt accacccctg ctaacggttt cactgtcaaa
    10381 gacctgtacg aagcgatttt agagctgttc gaccaggagc tttaatctct gccccgtcgc
    10441 ttctggcggc ggggaaaatg ttgcttatcc ctctcaaccc cctgctttcc cctgcgatta
    10501 atttaacgaa tagtgcgttt tactgcgaca tgtcattcac acaat
//
LOCUS       DAZUCE010000182        10451 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZUCE010000182 DAZUCE010000000
VERSION     DAZUCE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.182"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            46..2931
                     /locus_tag="ACHY03_003854"
     CDS             46..2931
                     /locus_tag="ACHY03_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS3907032.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNGVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVASGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVVMNNSVWNVTSNSNLDTLTLSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPTPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDEMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGKSQLNIYVKTGAIHEFSGDTEYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3031..4950)
                     /gene="dhaR"
                     /locus_tag="ACHY03_003855"
     CDS             complement(3031..4950)
                     /gene="dhaR"
                     /locus_tag="ACHY03_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFS3907033.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSNGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAVL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVCDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5178..6248
                     /gene="dhaK"
                     /locus_tag="ACHY03_003856"
     CDS             5178..6248
                     /gene="dhaK"
                     /locus_tag="ACHY03_003856"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFS3907034.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVTG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6259..6891
                     /gene="dhaL"
                     /locus_tag="ACHY03_003857"
     CDS             6259..6891
                     /gene="dhaL"
                     /locus_tag="ACHY03_003857"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFS3907035.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            6902..8320
                     /gene="dhaM"
                     /locus_tag="ACHY03_003858"
     CDS             6902..8320
                     /gene="dhaM"
                     /locus_tag="ACHY03_003858"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFS3907036.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDSCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSAVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            8640..10337
                     /gene="treA"
                     /locus_tag="ACHY03_003859"
     CDS             8640..10337
                     /gene="treA"
                     /locus_tag="ACHY03_003859"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFS3907037.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPT
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTIRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQKEAEPTP"
BASE COUNT         2732 a         2648 c         2617 g         2454 t
ORIGIN      
        1 gacaaaatcc atagacccat agcctgggta ctatatagga gtggtatggg catcaaacaa
       61 cacaatggga ataccaaagc cgatcgtctc gctgaattaa aaatccgttc gccctcaatt
      121 caactgataa aatttggcgc tattggtttg aatgcaatta tcttttcccc cctgctgata
      181 gctgctgata caggaagtca atatggcacc aatattacta ttaatgatgg tgacagaatt
      241 acaggagata ccgccgatcc atcaggaaac ctctatggtg taatgacccc agcaggaaac
      301 acgcctggca atatcaacct gggtaatggt gtcaccgtca atgtcaacga cgcctctgga
      361 tatgcaaaag gaatcattat tcagggcaaa aacagctccc tgacagctaa ccgactcaca
      421 gtagatgttg ttggtcaaac ctctgccatc ggcattaatt taattggtga ctatacccat
      481 gctgacttag gcacaggcag caccattaag agtaacgatg acggcatcat tattgggcat
      541 agctcaacac taacagccac tcaattcacc attgaaaact cgaacggtat aggcctaacc
      601 atcaatgact atggcaccag tgtcgatctt ggaagcggaa gtaaaatcaa gaccgatgga
      661 agtacaggtg tttatatcgg tggtctcaac ggcaataacg ccaatggtgc tgcgcgtttt
      721 acggcgacag acctgacaat cgatgttcag ggctacagcg ccatggggat aaacgtacag
      781 aaaaactctg ttgtcgatct cggaacaaac agtaccatta aaaccaatgg cgataatgct
      841 cacggcctct ggagctttgg ccaggttagc gcgaatgcac tcactgttga tgtaactgga
      901 gccgcggcca atggcgtcga agttcgtggt ggtacaacca ctatcggtgc agatagccat
      961 atttcttccg cgcagggcgg tggtctcgta gccagtggtt cagacgcgac aatcaatttt
     1021 tctggcacgg cagcgcaacg aaacagcatc ttttccggcg gttcttatgg tgcctcggcc
     1081 cagacggcaa cggctgttat caacatgcaa aatacggata ttacggttga tcgtaatggc
     1141 agtctggcgc tgggtttgtg ggcgctcagc ggcggtagaa taaccggaga cagtttggct
     1201 atcaccggcg cggcaggagc cagggggatt tatgccatga ccaacagcca gatcgacctc
     1261 acgagcgatc tggtcattga tatgagtaca cccgaccaga tggccatcgc aacgcaacat
     1321 gacgatggtt atgccgccag ccgcatcaac gcctcgggtc gtatgcttat caacggtagc
     1381 gttctttcca aaggtgggct aatcaatctg gatatgcacc ctgggtcggt ttggacaggt
     1441 tcctccctca gcgataatgt caatggcggg aaactggacg ttgtaatgaa taacagcgtc
     1501 tggaacgtaa caagtaattc taatctcgac acgctgacgc tgagccattc aactgtcgat
     1561 tttgccagcc acgggtcaac tgccggcaca tttaccacat taaacgtaga gaacctgagc
     1621 ggtaacagta cctttattat gcgtgctgat gttgttggcg agggtaatgg cgttaataat
     1681 aaaggggatt tattgaatat cagcgggagt agtgctggta atcacgtatt ggctatccgc
     1741 aaccagggca gcgaggccac aacgggaaat gaagttctga cagtggtaaa aaccactgac
     1801 ggtgcggcct cgttcagcgc gtcttctcag gttgagttgg ggggatatct gtacgatgtg
     1861 cgtaaaaatg gcactaactg ggagctttac gcttccggga cagttccaga accgactcct
     1921 aatcctgaac caacaccagc tcccgctcag cctcccatag tcaaccccga tcctacgcct
     1981 gaacccgatc ctacgcctac ccccactccc acacctaaac ccaccacgac cgcagatgct
     2041 ggcggcaatt atctcaatgt tggttactta ttgaactatg ttgaaaaccg tacgctgatg
     2101 caacggatgg gtgacctgcg aaatcagagt aaagacggta atatctggct gcgcagttat
     2161 gggggaagcc tggactcctt cgccagtggc aaactgagcg gctttgacat gggttacagc
     2221 ggtatccagt ttggtgggga taaacgtctc tctgatgaaa tgccgttgta tgtcggtctg
     2281 tatattggct caacacatgc atcgccggac tatagcggtg gcgacggtac cgcacgttca
     2341 gactacatgg gaatgtacgc cagttacatg gcacacaacg gtttttacag cgatctcgtt
     2401 gtaaaagcat cgcgccagaa aaatagtttc cacgtactgg acagtcagaa caacggcgtt
     2461 aacgccaacg gcactgcgaa tggattgagc atctccctgg aagccgggca gaggttcaac
     2521 ctgaccccta ctggttatgg gttctatata gagccgcaaa cccagctcac atacagccac
     2581 cagaatgaga tggctatgaa ggcgagtaat ggtctcaata tacatctgaa tcactacgaa
     2641 tcgctgctgg ggcgtgccag catgatactg ggatatgaca tcaccgcagg taaaagccag
     2701 ctgaatatct atgtgaagac tggcgctatc cacgagtttt caggggatac agaatatctg
     2761 ttgaacaact cccgggagaa gtacagtttc aaaggtaatg gctggaataa cggcgtggga
     2821 gtcagtgcac agtataacaa acagcacaca ttctacctcg aagcggatta cacgcagggt
     2881 aacctctttg atcagaagca agtcaacgga ggatatcgct tcagctttta agtgcgggat
     2941 agacaagccc ttaaacgatg ctgaaaaatg ttagcgtgcg ccggaatgaa atatagatca
     3001 ctctaatgta tcagaaatcg tctctgtctt tcatacccgg cgcttaaact gccctgcatc
     3061 aatgccatgt tgcttcattt tccgccacag cgtagtgcgg ccgattccaa gtaaagccga
     3121 catttcctga atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct ctttttcaac
     3181 ttccgcaaat gacagactgg tggaaaggcg tgtggcgctg acatcatctg tcgcctgctc
     3241 ggtaaacaga tgttccggca aatcacagac gcgaatgcgc ccattatcac tactcagagc
     3301 aagattctcg atgacgctgt aaagttcaaa atcgttgcca ggccatgcac aagaaaccag
     3361 gcgagcgagg gcatcgtcat caattttcag ccgcgtagag aagcgttttt caagactgcg
     3421 taatttgtta ttcaccaacg ccggaatgct gccacgccgc atacgcagag gcgggatggt
     3481 aatttcaaat gcatgcagcg cgtaatacag ctggcgacta aaacgatttt gttccaccag
     3541 cattgcgagg tccgcggtgg ttgtagcaat cactttgaca tcaattggta ttaaacgccg
     3601 cgcatccagt cgcgtgataa caccctgctt gataacctga agcaaagcag actgtaactc
     3661 caccgccaga tattcaatct tttcaagaaa cagcgtgccg ccgtgcgcca gttccagccg
     3721 actcagacgg ccattttcat tgtccgtgcg atcgccgcca ataaattctt ccgccagcac
     3781 cgcatcacca tataactcac aattgacggc gatataagga cctgcagcac gctcgctttc
     3841 attatgaatt gcctgactta gcagtgcctt gcccacgcct tcttctccac aaagcaggac
     3901 aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg tttgcggatc
     3961 gtcctgtggc atatgagcga aggtatggct gacttttcct aattgactgg tcattaactg
     4021 ccgcatctgt tccacaggat ggagcaacaa aataaagctg gttccctgcg tttctattat
     4081 cggtttaagg gttatcaccg catcaataaa ctggtgctgg ctttcaaagg ttgcttctac
     4141 gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat acggcgggta acgttaagag
     4201 ttcagtgatt gcccgcccct gacttgccgt cgcgtcaagg cgcaagaccc gcgccgcctg
     4261 ggcattaata aattgcaaat taccctgctc gtcccagcta atcacgccat catccatact
     4321 ttctaacagg gcattaagtt gatttaaatg acggttagtt tcagcgagca aactgtccgt
     4381 cagaagtaaa ttaccgacct cgcgggcgat tgccaacgtc aacggcaaat cagctgcggt
     4441 agtttgctca accggacacg ccagcgctat tgttcccgtc aatcggccgt tgctgtcaaa
     4501 caacggcgtt gcgcaaaagg cccagttcca gagtgcctgt ttgaaatgtt gatcggccat
     4561 cgttttcacg gcctgaccag agatagccgc taacgatagc gcacaagtac caataattcc
     4621 ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt agctgctgca aggtttgcgg
     4681 atcgccatta cggctgagaa tgcaggcggt ttcatcgagg ataaacagcg cacactctcg
     4741 cggtgccata tattcccagg catcttccag agcagcctgc ccgagcgtca gcatcgcttt
     4801 cttacgccga taaatagaag caaatgtcac gccctgggcc tggtgcggta cgctccatgt
     4861 ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt gcaattaagg gagatatgcc
     4921 ccgaccatcg ttgttaaaag cgccactcat atcccgcatc ccttatgaca aaaggtgttc
     4981 cggctcgcaa ttttaagtat taatacacgt aaaagtttca atatggaaca ttcattaaag
     5041 catatgtttc actatgaaac ataatttggg ctaattttct tttcccttgc ctgatgcaca
     5101 atggattcat cggcagtcag tggtcgccgt gtcgttgaac atcatccatg ccctaccgta
     5161 attgctggag caaaataatg aaaaaattga tcaatgatgt gcaagacgta ctggacgaac
     5221 aactggcagg actggcgaaa gcgcatccat cgctgacact gcatcaggat ccggtgtatg
     5281 tcacccgagc tgatgccccc gttacaggaa aagtcgccct gctgtcgggt ggcggcagcg
     5341 gacacgagcc gatgcactgt ggttatatcg gtcaggggat gctttcgggg gcctgtccgg
     5401 gcgaaatttt cacctcaccg acgcccgata aaatctttga atgcgccatg caagttgatg
     5461 gcggcgaagg tgtactgttg attatcaaaa attacaccgg cgatattctt aacttcgaaa
     5521 cagcaaccga gttactgcac gatagtggcg taaaagtgac cactgtggtc attgatgacg
     5581 acgttgcagt aaaagacagt ctttataccg ccgggcgacg cggcgttgcc aacaccgtat
     5641 taattgaaaa actcgtaggc gcagcggcgg agcgtggcga ctcactggac gcctgtgcgg
     5701 aactggggcg taagttgaat aatcaaggcc actcaatagg tatcgctctc ggtgcctgta
     5761 ccgttcctgc cgcgggcaaa ccttctttta ccctggcgga caatgagatg gagtttggcg
     5821 tcggcattca tggtgagccg ggtattgacc gtcgcccctt ctcttccctt gatcaaaccg
     5881 tcgatgaaat gttcgacacc ctgctggaaa atggctcata ccatcgcact ttgcgtttct
     5941 gggattatca acaaggcagc tggcaggaag aaccacaaac caaacaaccg ctccagtctg
     6001 gcgacagggt gattgcgctg gttaacaatc ttggcgcaac tccactttct gagctgtacg
     6061 gcgtctataa ccgcctgacc acacgttgcc agcaagcggg attgactatc gagcgtaatt
     6121 taattggcgc gtactgcacc tcactggata tgaccggttt ctcaatcacc ttactgaaag
     6181 ttgatgacga aacgctggca ctctgggacg ccccggtcca caccccggcc cttaactggg
     6241 gtaaataagg agaaagcaat gtcactgagc agaactcaaa ttgttaactg gctcactcgt
     6301 tgtggcgata ttttcaacac cgagagcgag tatcttaccg gactggatcg cgagattggc
     6361 gacgctgacc acgggctaaa tatgaaccga ggctttagca aagtggtgga aaaactccct
     6421 gctattgcag ataaagatat cggtttcatt ctcaagaata ccggtatgac gctgctttcc
     6481 agcgtcggtg gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc agcacaggcg
     6541 acccaggcac ggcaaagcct gacgctggaa gagctttatc agatgttccg cgatggcgcg
     6601 gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg tgatgtgtgg
     6661 gtgccggtgg tggaatcatt acgtcagtcc agcgagcaaa atctctctgt tccggcggcg
     6721 ctcgaagctg ccagtagcat cgccgaatcc gctgcacaaa gtacgattac gatgcaagcc
     6781 cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg gtcaccagga tcccggcgca
     6841 acctcggtga tgtttatgat gcaaatgttg gcgttagccg caaaagagta aggaattggt
     6901 gatggtaaac ctggtcatag tttcacatag cagccgactg ggagaaggtg tcggtgaatt
     6961 agcccgtcag atgttaatga gtgatagttg taaaatcgcc attgccgcgg gaattgacga
     7021 tccacaaaat cccattggta ccgatgccgt caaagtgatg gaggccatcg aatctgttgc
     7081 tgatgccgac catgtgctgg tcatgatgga tatgggtagc gcattattga gtgctgaaac
     7141 tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta cgtttgtgtg ctgcgccgtt
     7201 ggtcgaaggt acactggcag caacggtcag cgcggcctcg ggggcggata tcgacaaagt
     7261 tatctttgac gccatgcatg cgctggaagc caaacgtgaa caactgggtt taccgtcctc
     7321 cgacactgaa atctctgact catgtcctgc gtacgatgaa gaagcccgtt ctctggcggt
     7381 ggtcattaaa aaccgtaacg gcctgcacgt ccgtccggcc tcccggctgg tttatacctt
     7441 atcgacattt aatgccgata tgttgctgga aaaaaacggc aaatgcgtca caccggagag
     7501 tattaaccag attgcgttac tacaagttcg ctataacgat acgctgcgcc tgattgcgaa
     7561 agggccagaa gctgaagagg cactgatcgc tttccgtcag ctggctgaag ataactttgg
     7621 tgaaacggag gaagttgctc cacctactct gcgtcccgtt ccgcctgttt cgggtaaagc
     7681 cttttattat caaccggttt tatgtacggt acaggccaaa tcaaccctga ccgtggaaga
     7741 agaacaagaa cgattacgcc aggccattga cttcacgtta ttagatctga tgacgttaac
     7801 agcgaaagca gaagccagcg ggcttgacga tattgccgca atcttttctg gtcaccatac
     7861 actgttagat gatccggaac tgctggcggc ggcaagcgaa ctccttcagc acgaacattg
     7921 cacggcagaa tatgcctggc agcaagttct taaagaactt agccagcaat accagcaact
     7981 ggatgatgaa tatctacaag ctcgctatat tgatgtggac gatcttctgc atcgcaccct
     8041 ggtccacctg acccaaacga aagaagaact cccgcagttt aactcgccaa ctattctact
     8101 ggcggagaac atttatccct ccgcagtact gcaactggat ccggcggttg taaaaggtat
     8161 ctgccttagc gccggaagtc cggtatccca cagcgccctg atcgcccgtg aactggggat
     8221 tggctggatt tgccagcagg gtgagaaact gtatgcgata caaccagaag aaaagctaac
     8281 gctggacgtt aaaacgcaac gtttcaaccg tcagggttaa gtcaccgttc ggatggcatc
     8341 gttctgatgt catccggcaa tttactcagc actaatgttt tttaactacc tgttatattt
     8401 ttcaccaatt tatgatgatt tttcttcgct aatcgcgaac caaacgccgc tggctggcta
     8461 ataaacagac gttatttgat cactctattt cgccgttatt tatcccttta attccctttt
     8521 ctaaaatgcc tgacagttcg cagaatgaga tttcgatcat gcagctagtg cgatcctgaa
     8581 ctaaggtttt ctgatacttg aataccgttt tattcggttt cgcctaagga gaatgattga
     8641 tgaaatcccc cgcaccttct cgcccgcaaa aaatggcgtt aattccagcc tgcatctttt
     8701 tgtgtttcgc tgcgctatcg gtgcaggcag aagaaacacc ggtaacgcca cagccgcctg
     8761 atattttatt agggccgctg tttaatgatg tgcaaaacgc caaacttttt ccggaccaaa
     8821 aaacctttgc cgatgccgtg ccgaacagcg atccgctgat gatccttgct gattatcgga
     8881 tgcagcaaaa ccagagcgga tttgatctgc gccatttcgt taacgtcaat ttcaccctgc
     8941 cgacagaggg cgagaaatat gttccgccag agggacagtc actgcgcgaa catattgacg
     9001 gactttggcc ggtattaacg cgttctaccg aaaacaccga aaaatgggat tctctgttac
     9061 cgctgccgga accttatgtc gtgccgggcg gacgttttcg cgaggtatat tactgggaca
     9121 gttacttcac catgttagga cttgccgaaa gcggtcactg ggataaagtc gcggatatgg
     9181 tggccaattt tgctcatgaa atagacactt acggtcatat tcccaacggc aaccgcagtt
     9241 actatttaag ccgctcgcag ccgcccttct ttgccctgat ggtagagtta ctggcgcagc
     9301 atgaaggcga tgccgcattg aagcaatacc tgccgcaaat gcaaaaagaa tatgcttact
     9361 ggatggacgg tgttgaaaac ctgcaagccg gacaacagga aaaacgcgtt gtaaaacttc
     9421 aggatggtac ccttctcaac cgctactggg acgatcgtga tacgccacga ccagagtcat
     9481 gggtggaaga tattgccacc gccaaaagca atccgaatcg acctgccact gaaatttacc
     9541 gcgacctgcg ctctgccgct gcgtctggct gggatttcag ctcgcgctgg atggacaatc
     9601 cgcagcagtt aaataccata cgcaccacca gcatcgtacc ggtcgatctg aacagcctga
     9661 tgtttaaaat ggaaaaaatc ctcgcccgcg ccagcaaggc tgccggagat aacgcgatgg
     9721 caaaccagta cgaaacgctg gcaaatgccc gtcaaaaagg gatcgaaaaa tacctgtgga
     9781 acgatcaaca aggctggtat gccgattacg acctgaaaag tcataaagtg cgcaatcagt
     9841 taaccgctgc cgccctgttc ccgctgtacg tcaatgcggc agcgaaagat cgcgccaaca
     9901 aaatggcgac ggcgacgaaa acacatctgc tgcaaccagg cggcctgaac accacgtcgg
     9961 tgaaaagtgg gcaacaatgg gatgcgccaa atggctgggc accgttgcag tgggtcgcga
    10021 cagaaggatt acaaaactac ggtcaaaaag aggtggcgat ggacattagc tggcacttcc
    10081 tgaccaatgt tcagcacacc tatgaccggg agaaaaagct ggtggaaaaa tatgatgtca
    10141 gcaccaccgg aacggggggc ggcggtggcg aatatccatt acaggatggc tttggctgga
    10201 ccaatggcgt gacgctgaaa atgctggatt tgatctgccc gaaagagcaa ccgtgtgaca
    10261 atgttccggc gacgcgtcca ctcagtgaat caacaacaca gccggtaaaa caaaaagagg
    10321 cggaacctac gccttaactt tgctgtatat cgagcaatgc cattatgtgc ataaaattgg
    10381 cattgctctt ttttcgccac gaaatgcccc ccattctacg gtctatgcct ttattttctt
    10441 ttatcagaga a
//
LOCUS       DAZUCE010000183        10375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZUCE010000183 DAZUCE010000000
VERSION     DAZUCE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.183"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(214..1020)
                     /gene="trpA"
                     /locus_tag="ACHY03_003860"
     CDS             complement(214..1020)
                     /gene="trpA"
                     /locus_tag="ACHY03_003860"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFS3907038.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(1020..2213)
                     /gene="trpB"
                     /locus_tag="ACHY03_003861"
     CDS             complement(1020..2213)
                     /gene="trpB"
                     /locus_tag="ACHY03_003861"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFS3907039.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2225..3586)
                     /gene="trpCF"
                     /locus_tag="ACHY03_003862"
     CDS             complement(2225..3586)
                     /gene="trpCF"
                     /locus_tag="ACHY03_003862"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFS3907040.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSSRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDGG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGKTLPARELQHVDKYVLDNGLGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3587..5182)
                     /gene="trpD"
                     /locus_tag="ACHY03_003863"
     CDS             complement(3587..5182)
                     /gene="trpD"
                     /locus_tag="ACHY03_003863"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFS3907041.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5182..6744)
                     /gene="trpE"
                     /locus_tag="ACHY03_003864"
     CDS             complement(5182..6744)
                     /gene="trpE"
                     /locus_tag="ACHY03_003864"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFS3907042.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRI
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLAAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LAEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETSNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6766..6859)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6836..6880)
                     /gene="trpL"
                     /locus_tag="ACHY03_003865"
     CDS             complement(6836..6880)
                     /gene="trpL"
                     /locus_tag="ACHY03_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFS3907043.1"
                     /translation="MKAIFVLKAWWRTS"
     gene            7018..7899
                     /gene="rnm"
                     /locus_tag="ACHY03_003866"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             7018..7899
                     /gene="rnm"
                     /locus_tag="ACHY03_003866"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFS3907044.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7896..8516
                     /gene="yciO"
                     /locus_tag="ACHY03_003867"
     CDS             7896..8516
                     /gene="yciO"
                     /locus_tag="ACHY03_003867"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFS3907045.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8544..>10375
                     /gene="yciQ"
                     /locus_tag="ACHY03_003868"
     CDS             8544..>10375
                     /gene="yciQ"
                     /locus_tag="ACHY03_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFS3907046.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENKEMPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQSDKNQQILIRV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALPGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQMHLSLGANSSLLG"
BASE COUNT         2442 a         2674 c         2734 g         2525 t
ORIGIN      
        1 taagtgactt agagaaaatt attgatttta ctggtgttat gttgcgggat taatttgttt
       61 gattatatta ctgttgggcg gaaaaatgac gtaagttgac gttcgacagg ggtaagcgaa
      121 acggcaaaaa gataaatatt aaatgaattt aggatttttc cggcttcgtt aaagaaagtt
      181 aaaatgccgc cagcggaact ggcggctgtg ggattaactg cgcgtcgccg ctttcatcgg
      241 ttgtacaaaa actttcagtg ccgccagcat tttctctggc tcattaatat gttgctcgat
      301 gattttaaca atggccgaac cagaaatcgc gcccgcagct cctgcatcaa tcgctgcttt
      361 tacctgatcc ggggcggaaa taccaaatcc ctgcaatgga ggtgcagcgt tgtactcttt
      421 cagcttcgca accagatgat tgaggggtaa cgcggcgcgg ttttctgcgc cggtcacgcc
      481 cgctcgtgac agcaaatagg tgtaaccacg accgtaagag gctatctggc gcagcaggtc
      541 gtcatcggca tttggcgggc agatgaagat aggtgcgaca ttatgacgca acgcggcctg
      601 gcggaagggc gcggactctt caactggcac atcggcaaca agcaccgaat cgacgccgac
      661 tttttcgcac tgggcataaa actcatcaat gcctttgtta aacaccagat tggcatacat
      721 caacaggcca atgggaatgg tcgggtgttt ctggcgaatc agtgccagca tttcaaaaca
      781 ttgtgccgga gtcacacctg ccgcaaaggc gcgcagagtg gcgttttgaa tggtcgggcc
      841 atccgccagt gggtcggaga aggggatacc taactccagc gcgtcagcac cggcttcaat
      901 tagcgtatcg ataattttca aggactgctc aatgcccgga tccccgagcg tgacgaaagg
      961 aacgaatgcg ccttctttgc gctccttcaa ctgggcaaac agagattcgt agcgttccat
     1021 cagatttccc ctcgtgcttt caaaatatcg tgaacggtga agatgtcttt atcgccgcga
     1081 ccggaaaggt taaccaccag tagctgctct ttttccgggt tttcgcgcat cattttcagc
     1141 gcatgggcca gggcgtggga ggattccagc gccgggatga tcccttcgtg caggcacagc
     1201 gttttgaagg cttcaagggc ttcatcatcg gtaatagaca cgtaatcagc gcgtccagtg
     1261 ctgttaagat acgcgtgttg tgggccgaca gacgggaaat ccagcccggc ggagatggag
     1321 taagattctt caatctgccc gtcttcggtt tgcatcatcg gcgctttcat accgaaatag
     1381 atgcccacgc gaccatgttt taacggtgcg ccgtgctcgc cggtttctat accgtgaccg
     1441 ccaggctcca caccaatcag gccgacgtcg gtttcgttga tgaaatctgc aaacatgccg
     1501 atggcattcg aaccgccgcc aacacaggcg ataacggcat ccggcaggcg accttctctt
     1561 tccagaatct gtgctttggt ttcttcgcca atcatccgct gaaactcacg gacaatggtc
     1621 ggataaggat gtgggccagc tgcggtgccc agcatatagt gcgcggtttc ataactgccg
     1681 gaccagtcgc gcagcgcctc gttacaggca tctttcagcg tcgcggaacc gctatgcacc
     1741 gggatcactt ccgcacccat taaacgcata cgaaaaacgt taggcgactg gcgttcaacg
     1801 tctttggcac ccatataaat acggcatttc aggccgagca gggcgctggc aagggccgac
     1861 gccacgccat gctgaccggc tccggtttcg gcgatgattt cagttttacc catccgcttc
     1921 gccagcaacg cctgccccag cacctggttg gttttatgcg cgccgccgtg cagcaaatct
     1981 tcgcgcttca gatacagcgt ggtgttcgtc ccagccgtaa tgttctggca tttggtcagc
     2041 gctgttggac gcccggcata gtttttcagc aggtcgttga actgagcctg aaattcagga
     2101 tctttttgcg cgctgacaaa agcttcttcc agctggcgca gagcaggcat caggatttgt
     2161 ggcacgtaca tgccgccaaa ctcaccaaaa taggggttaa gtaatgttgt cattgttcct
     2221 ttccttaata tgcgcgcagc gtctggaaaa ccgaggccaa aagacgtgcg tctttgatgc
     2281 ccggttgcga ctctacagca gaattaaaat caagtccggc gcagccggtt tgtgccgctt
     2341 ccacgcagtt atctgcgcct aagccccccg ccagcagaac gttgccaagg gattgaccgt
     2401 ttaacagtga ccagtcgaaa cgttgcccgc ttccacccag gccgttgtct aaaacatatt
     2461 tatcaacgtg ctgaagttcg cgggcgggca gggttttacc gacgcttaat gctttccaga
     2521 tggcaacgtg tgctggcaga gcttcacgca gcgtatcgat atacagctga tcttcattac
     2581 catgcagttg cactgccgcc agcgataaca ccttagcttt gtccaacaca tcggcaatat
     2641 cgtgattgcg gaacacgcca acatactgca acggtgccgc agccatcact tcctgcgcct
     2701 gttcaacgtt gacgcaacgc ggtgatgtcg caacaaaaat caacccaccg taaatcgcgc
     2761 ccccgtcata agctgcttta gcatcttgcc cacgcgtcag gccacatact ttattctcac
     2821 ccagcaatac ccggcgcacg gcggcgtgca aatcgtcatg ggccatcaac gccgaaccaa
     2881 tcagaaaacc gttagcgaag tggcttaact cgcgcacctg agcgtaagta ttgatgccgg
     2941 attcgctgat taccgtcacg ttgtgcccca gtttcggcgc aagctcgcgg gtacggttga
     3001 gatcaatcga caaatcacgc agatcgcggt tgttgatgcc aacgaccttt gcccccaatg
     3061 caatggcgcg ctccagttcc tcttcattac tgacttcagt cagcacaccc atctccagac
     3121 tgtgggcgac ggctgcaagc tggcgatatt ggtcgtcatc cagtactgaa agcattaata
     3181 agcaggcatc ggcctggtaa tagcgcgcca gatagatctg gtaagggtcg ataatgaagt
     3241 ctttacataa aatcggctgc ggggcgattt ggctgacgat ggggaggaaa tcaaagctcc
     3301 cctgaaaata tttctcatca gtcagcaccg aaattgccga agcgtaatgt ttataaatgg
     3361 cggcaatgcg tgctggatcg aaatcatcac ggatcacgcc ttttgacggc gacgctttct
     3421 tgcactccag aataaacgcc gtgcgcgcac cctgtagcgc atcataaaaa tgtcgcgagc
     3481 taggctgaac ctcattctga aaactggcca gcggttgctg ctgtttgcgg gcttctaccc
     3541 aaatcgcctt gtctgcgacg attttcgcta aaacggtttg catcatttac cctcgtgccg
     3601 ccagtgcggt gactctgtcg taagcggaac cactgcgcag tacctcaaga acggtttgcg
     3661 cattggcttg cagatcttca tggccatgca ggcgcattaa catggcgacg ttcgcagcga
     3721 cggctgcttc atgggcggcg tcgcctttac cttgtaacaa acgtgttaaa atgtcacggt
     3781 tttcttccgg tgttccgcct gccagttgct cctggtggta gggtgtcagg ccaaaatctt
     3841 cagcggtcaa ttgatagctc ttaatttcgc cgtcatgcag ctcggcaacg attgtcggcg
     3901 cgtgtaatga aacttcatcc atcccgccgc tgtgcaccac cgccgcgcgt tgatacccca
     3961 gcacgcgcaa ggtttcggca atcggcagca ccagttccgg actataaaca ccaattaacg
     4021 ccagcggcgg atgcgccggg ttaatcaatg gtcccagcac gttgaacaag gtgcgggttt
     4081 tcagttgctg gcgaaccggc atcgcgtggc ggaatccggt gtgatacttc ggcgcaaaga
     4141 ggaaacatac gcctaactca tccagcgcct ggcgcgattt atcggcgttc atatcaagat
     4201 taataccgaa cgccgccagc agatccgacg agccagattt actggagacg ctgcggttgc
     4261 cgtgttttgc gactttcagc ccacaggccg cggcgacaaa cgcactggcg gtagaaatat
     4321 tgatgctgtt gctgccgtca ccgccggtgc cgacgatatc ggcaaacaga taatccgggc
     4381 gcgggaacgg cgcggcattt tccagtagcg cggttgctgc cccggcgatt tcgtttgggt
     4441 gctcgccgcg aattttcatg ctcaccagcg ccgccgccag ttgttccggc ttcagctcgc
     4501 cacgtaccac cgctgaaaac agctggtggc tttcttgttg gctaagcgtc tgcgcctgat
     4561 acagtttttc cagaatcggt tgcagcgtgt tggttggctc tagtttctgc tgcgcccagg
     4621 ccagcgtttg ttccagcagg cgagcgccct gggtagtaag aatggattcc ggatggaact
     4681 ggaatccaca aacgcgatcc gcatcgtgac gtactgccat caccatgcca ttaaaatggg
     4741 cgttgatggt taaaccggcc ggaatattac tgccaaccag cgagtgataa cgcgccaccg
     4801 gcaatgggtt tgttaatccg gcaaacatcg cctgaccgtc atgttcaatg ctggaggctt
     4861 taccgtgaag aatttcgccc gcctgaccga catacccccc gtaggcttcg acaatcgcct
     4921 gatgaccgag gcaaatgcca ataatcggca gcttaccgcg caagcgggtg aggagttctg
     4981 gcatacaacc ggcttcactc ggcacacctg gccccggaga aagcatcagc accggattgc
     5041 tcatcgtcgc caggcgttca attaaggttt gcgccggaat atggttgcgg taaatcacca
     5101 cgttatgccc attgctgcgc aactgatctg ccaggttgta agtaaaagag tcgatattat
     5161 cgagcagcag aatgtcagcc atcaaaaagt ctcctgtgca tgatgcgcgg tggcgatagc
     5221 gcgcagtaca gcgcgggctt tattactggt ttcgtcggct tccgactgcg gaacagaatc
     5281 aaggactacg ccagcaccgg cttgcacggt ggcgataccg ttttccacca gcgccgagcg
     5341 gatcacaatg caggtgtcga gatcgccatg cgcggtgaaa taacctaccg cgccgccgta
     5401 gctgccgcgg cgacgacctt ccgcctcggc aattaactgc atagcacgca ctttcggcgc
     5461 accgcttaac gttcccatat tcatacaggc gcggtaagcg tgcagggcgt cgagatcgtg
     5521 gcgcagttcc ccgaccactc gggagaccag gtgcatcacg taagagtaac ggtcaacttt
     5581 ggtgagatcg gcgacgtagc ggctgccggg ggtgcaaatg cgtgccagat cattacgggc
     5641 gagatccacc agcatcagat gttcagaaag ctctttatga tcggtacgca tttccagttc
     5701 gatgcggctg tcgaggtctc tgtccagtga accatcggca cgacgaccgc gtggacgtgt
     5761 tccggcaatc gggtagatct caatctggcg gctggtggcg tcatacttga gcgaactttc
     5821 cggcgacgcg ccaaacaggg tgaaatcatt atcctgcata aaaaacatgt acgggctggg
     5881 attactcttt ttcaacacgt aataggccgc cagcggtgac gggcagggca gcgagaaacg
     5941 gcgagatggc acgacctgga aaatttctcc ggcgcgaatc gctttttgca acaaacgcac
     6001 tacgccaccg aactcttcat cgctctggtt acattcacaa cgcatatgcg gcacggaaac
     6061 caccggcagc ggcggcgcgg cttcggccag ttgctgacgt agttcgttca ggcgagcagt
     6121 gagacgttgt ttttcttctt cattcggagc aaacagactg gcctgaatac gggtgctttt
     6181 tttctgatgg tcaatcacca tcagagtttc agcgagataa aaacagaaat cagggcagtt
     6241 attttccgct gccagttgcg gtaaatcttc aaatcccgcc accagatcat aagagaacag
     6301 gccaccaaag aacatcgctt cccgttcttc cttcggtaca ttcaacagat tctgcaataa
     6361 gcggaaagcg tcaaaaaccg aaagagagca taaacgggca tcttcatcca gcagtggact
     6421 gaccgggggg aagcgcagta tacggcagtt tggtgattgt tcattttcca cacccgcagg
     6481 aagggcgtta tccagtagtg tcagcagggc ttcgccattg ccggaaagcg cctggattgt
     6541 gacagtgtca cctaatgctg taatgcgcag cgcactgtct accagcagca ggctttttaa
     6601 atcatccttg ctgtcgatat ctgcggattc cagcagcagc gtcgcagggc gatccccaca
     6661 caactggtga aaaagcgcgg tcgggttgtc gcgataagcg ccttcgcagg ttagcagttc
     6721 gagagtcggt ttttgtgttt gcattgttat tctctaattt tgttcaaaaa aaagcccgct
     6781 cattaggcgg gctgggtatc tgattgcttt acgcatggtg aatacactgc ccgtttcagg
     6841 aagtgcgcca ccaagctttc agtacgaaaa ttgctttcat tgtcgatacc ctttttacgt
     6901 gaacttgcgt actagttaac tagttcgatg attaattgtc aacagctcat ttcagaatat
     6961 ttgccagaac cgttatgatg tcggcgcaaa aaacattatc cagaacggga gtgcgccttg
     7021 agcgacacga attacgcagt gatttacgac ctgcacagcc ataccacagc ttccgatggc
     7081 tgcctgacgc cagaagcatt ggtgcaccgt gcagtcgaga tgcgcgtcgg caccctggcg
     7141 atcaccgacc atgacaccac agctgcaatc gcgccagcaa gagaggaaat ttcacgttcg
     7201 ggactggcgc tgaatcttat tcccggcgtg gaaatttcca cggtctggga aaatcatgaa
     7261 attcatattg tcgggctgaa tattgatatt actcatccgc taatgtgtga gttcctcgca
     7321 cagcagacag aacggcgcaa tcagcgggcg cagcaaattg ccgaaagact tgaaaaagcg
     7381 caaatacctg gggcgctgga aggcgcacaa cgactggcgc agggcggcgc agtgacgcgc
     7441 ggtcattttg cgcgtttcct tgttgagtgc ggcaaagcca gttcaatggc ggatgtcttt
     7501 aaaaagtatc tggcgcgcgg gaaaaccgga tacgttccgc cacagtggtg tacaatagaa
     7561 caagctattg atgtcattca tcattctggc ggtaaggcgg tgctagctca tcctgggcgg
     7621 tacaatctta gtgctaaatg gctgaaaaga ttggttgcgc attttgccga acacgacggc
     7681 gacgcgatgg aagtcgcgca gtgccagcaa tcgcccaatg aacgcaccca gctggcgaca
     7741 cttgcgcgtc agcatcattt atgggcatca caaggatctg attttcatca gccatgcccg
     7801 tggatcgaac tgggtcgtaa actctggctg cccgcaggcg ttgaaggcgt ctggcagcta
     7861 tgggaacagc cgcagaacac cacagagagg gaattatgag ccagtttttt tatattcatc
     7921 ctgataaccc acagcaacgt ctgatcaacc aggcggtgga gatcgtgcgc aaaggcgggg
     7981 tgattgttta tccaactgat tccggctatg cgctcggctg taaaattgaa gataaaaacg
     8041 ccatggagcg tatttgtcgt attcgccagc tgccggacgg tcacaacttt accctgatgt
     8101 gtcgcgatct ttctgaactc tcgacctatt catttgtcga taacgttgcg tttcgtttaa
     8161 tgaaaaacaa cacgccgggc aactatacct tcatcctgaa ggggacgaag gaagtgccgc
     8221 gccgtctgtt gcaggaaaaa cgcaaaacca tcggtatgcg tgtgccttct aacccgattg
     8281 cgcaggcttt gcttgaagca ctgggcgaac cgatgctttc cacttcgcta atgctgccag
     8341 gcagcgaatt taccgaatcc gatcccgaag aaattaaaga ccgtctggaa aaacaggtag
     8401 atttgattat tcacggcggc tatctcgggc agaaaccaac aacggttatt gatcttaccg
     8461 atgatacgcc ggtcgtggtg cgtgaaggcg taggtgatgt gaagcctttc ttataacaaa
     8521 ttgcgttcca cggatggaag actatggcag ggaagtttcg ctgcattttg ctgttgatag
     8581 cagggctgtt tgtatcatct ctaagttatg cagaaaacaa ggagatgcct tcttatgaag
     8641 aagggatctc gctctttgat gttgaagcca ctctgcaacc ggatggggtg ctcgacatca
     8701 aagagaacat tcattttcag gcgcgaaatc agcagattaa gcacgggttt tatcgtgatt
     8761 taccacgact atggatgcag cctgatgggg acgctgcact gctgaactat catattgttg
     8821 gcgtcacccg tgatggtatt cctgaacctt ggcatcttga ctggcatatc gggttaatga
     8881 gtattgtcgt gggcgataaa caacgtttct tgcctcaagg cgactatcat tatcaaattc
     8941 attatcaggt taaaaatgct ttcctgcgtg agggagattc agatctgtta atctggaacg
     9001 tgactggtaa ccactggccg tttgaaatct ataagacccg attttcactc aagttccctg
     9061 atatcgcggg taatccattt agcgaaatcg atctctttac tggagaagag ggcgacacat
     9121 atcgaaatgg ccgcatcctt gaggacggaa gaattgaatc cagcgatccg ttttatcgtg
     9181 aagatttcac ggtactctac cgttggcctc actcgttact tagcaatgcc ccggctccac
     9241 aaacgacgaa tattttcagc catcttcttt taccctccac gtcatcgttg ttaatttggt
     9301 ttccgtgtct attcctggtt tgtggatggt tatatctctg gaagcgcagg ccgcaattta
     9361 cgccggtaga tgtgattgaa accgatgtca ttccgccaga ttacacgccc ggcatgttac
     9421 gtctcgatgc gaagctggtt tacgacgata aagggttttg tgccgatatc gtaaatctga
     9481 ttgtgaaagg aaaaattcat ctggaagatc agtctgacaa gaaccagcaa atcctgattc
     9541 gtgttaatga aggcgcgacc agaaataatg aggtattact gcccgcagag cagttattac
     9601 tggaagcgtt atttcgtaaa ggcgataagg tcgttcttac ggggagacgc aacagagtct
     9661 tacgcagggc atttttacgg atgcagaaat tttatctgcc gcgtaaaaag tcttcgtttt
     9721 accgacctga tgcgtttttg caatggggcg gaatggcaat attggcggtc attctctacg
     9781 gtaacctgag tcccgtaggt tgggcaggaa tgagtctggt gggcgatatg tttattatga
     9841 tctgctggct tcttcctttt ttattttgtt cccttgagct tttgtttgcc cgcgatgatg
     9901 acaagccttg cgttaatcgt gtaatcatca ctttgttttt accactgatt tgttcaggcg
     9961 tagccttcta ttctctctat atcaatgtcg gagatgtatt cttttactgg tatatgccag
    10021 cgggttattt tagcgctgtt ttcctgaccg gttatctcac tggcatgggg tatatttttc
    10081 tgccaaagtt tacccaaact ggacagcaac gttatgccca cggtgaagct atcgttaact
    10141 atcttgcgcg taaagaggcg gcaacgcaca gtgggcgtcg gcggaaaggg gaaacacgga
    10201 aactggatta cgcgttgcca ggttgggcgg tctcggcaaa ccttggcaga gaatgggcag
    10261 cacgtatcac cccatcactc acagcggctg ttcgcgcccc ggaaattgcc cgtagtggcg
    10321 ttttgttctc attgcagatg cacctgagtc tgggggccaa tagcagtttg ttggg
//
LOCUS       DAZUCE010000184        10244 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZUCE010000184 DAZUCE010000000
VERSION     DAZUCE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.184"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..117)
                     /gene="yeaR"
                     /locus_tag="ACHY03_003869"
     CDS             complement(<1..117)
                     /gene="yeaR"
                     /locus_tag="ACHY03_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFS3907047.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGV"
     gene            complement(290..928)
                     /gene="leuE"
                     /locus_tag="ACHY03_003870"
     CDS             complement(290..928)
                     /gene="leuE"
                     /locus_tag="ACHY03_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFS3907048.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1055..1978)
                     /gene="dmlR"
                     /locus_tag="ACHY03_003871"
     CDS             complement(1055..1978)
                     /gene="dmlR"
                     /locus_tag="ACHY03_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFS3907049.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2081..3166
                     /gene="dmlA"
                     /locus_tag="ACHY03_003872"
     CDS             2081..3166
                     /gene="dmlA"
                     /locus_tag="ACHY03_003872"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFS3907050.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGIPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            3417..5027
                     /gene="yeaV"
                     /locus_tag="ACHY03_003873"
     CDS             3417..5027
                     /gene="yeaV"
                     /locus_tag="ACHY03_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFS3907051.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5059..6183
                     /gene="yeaW"
                     /locus_tag="ACHY03_003874"
     CDS             5059..6183
                     /gene="yeaW"
                     /locus_tag="ACHY03_003874"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFS3907052.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            6239..7204
                     /gene="yeaX"
                     /locus_tag="ACHY03_003875"
     CDS             6239..7204
                     /gene="yeaX"
                     /locus_tag="ACHY03_003875"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFS3907053.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALISSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7258..8373)
                     /gene="rnd"
                     /locus_tag="ACHY03_003876"
     CDS             complement(7258..8373)
                     /gene="rnd"
                     /locus_tag="ACHY03_003876"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFS3907054.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHNISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(8455..10140)
                     /gene="fadD"
                     /locus_tag="ACHY03_003877"
     CDS             complement(8455..10140)
                     /gene="fadD"
                     /locus_tag="ACHY03_003877"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFS3907055.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
BASE COUNT         2532 a         2665 c         2502 g         2545 t
ORIGIN      
        1 aacccccggg cgcgttcctt tatcaagatg acgttcgaat attccggcag gcgcagtttg
       61 tttattccag aaaggcgttg agcgcgtatg aatataattc tgtgggattt gcagcatcct
      121 tttccctcct tcggtgaatg cgctgaaaac ggtttattcc agccgtttca gggtacgcct
      181 gataatttgc attttaaata ccatttattg gttacttttt agcaccatat cagagaagaa
      241 tcagggagga ttatagatgg gaagcccgcg acaacgcggg ctgaaagcat caggattgca
      301 gcgtcgccag tcgggcagcg aaacccacga acatcaaacc aatcagtgag ttgcccactt
      361 tagccagttt ctttttggta cgtatgtact gcgtgacaaa agcaccagaa ataatcagga
      421 agctcaaata gcagaaactc accagttcca gcgtcgtcgc cagaataaag aatgaaattc
      481 ccgtatgtgg ggcattaaca tcgataaact gtacgaaaaa cgacacatag aacaaaatgg
      541 ctttcggatt agtcaggctc aaaattaacg cgcgtttaaa aatagcaccg tattggggct
      601 catcggattt ggcctcgcta tttttaccct tcagggtcgc gtaaagaatt ttactcccca
      661 gatagagcaa ataaaacgca ccaagataac gtacaatgtt gaataatatc ggggtggtct
      721 taattaatgt cgccactcca gcccatgcca gaaacatcaa taccgcatcg ccaataaata
      781 caccgcaggc cgcaagataa ccgcctttca taccgctact gacgctattt ttgagtacaa
      841 acagggtatt tggccctggc accaacacaa taaaaatggc cccaaccaga taggtccagt
      901 aattcagaac cccgtattca gcgaacacat taacctcttt aattatcttt cgatcatgcg
      961 cgattaaagg tgaatatgct aaccaatctg tagcggctta gaaaggagaa aatcaggttt
     1021 taacctgata tcaacccgat aattgaatca tgaatcaggc atgctccagt gaaaaattcg
     1081 ggtagtgctc ggcaaaatac tgccgtaaaa attctaccgt tatccgcact ttcgctgacg
     1141 tcgccagcct tgaaacataa acggcccaga cgttcgctgg ctggtaatat tccggtagca
     1201 cttgcactaa atgaccactg gcaatgtttt cgctaacatc ccaccaggaa cgcagagcaa
     1261 tcccctgccc atcgagacac cactgatgga caatttctcc atgattagac gacagtgggc
     1321 cggtgacttt aatcgcatgg tggccttctt tattgcgtaa ttgccagaca ccaaaaggat
     1381 gatcgcgctc tttaatgacc aggcacggca atgcagaaag atcggttaaa tgtttcggcg
     1441 cgccatgttg cgcaataaat tccggcgacg cgcagagaat gcggtaattg gtcgccagtt
     1501 tgcgggcgat taaattaggg gcgatgtcat cgccaatgcg aatatcgaga tcgacacctt
     1561 cattgaccaa atcgaccagc cggtcttcca catcaaaacg taattccagt tgcggatacg
     1621 ccttcgccag cgctgataac gccggagcca ccacctgtcg tccaaaacca aagctgctga
     1681 taatacgcag catcccctgt ggcacctgac gcacgtcaga aagttcgtcc atcatctgac
     1741 cgacatcctg caaaatccgc tgcgcccatt cataaatccg ctctccttct tcggtaatgg
     1801 tgacgcggcg ggtggtgcgg tgtaacaaca ccacgtttag cgtttgctcc agcaaggcga
     1861 tgcgcttgct gacgaacgcc ggtgaaacgc ccagttcttc cgccacggcg gcaaaaccag
     1921 cccggcgagc caccagcata aagacgcgca aatcattcag cagcggtaaa ttattcatga
     1981 ttcgtgtttt atgtttcacc agttacaggg attaattcct tttcagtcaa ttataggatg
     2041 gtgatgttgt caattttgat ggtcaggaag tgagaaccca atgatgaaaa cgatgcgtat
     2101 tgctgcgatc ccgggagacg ggattggcaa agaagtcctt cctgaaggga ttcgcgtgtt
     2161 acaggctgcc gctgagcgct ggggcttcgc cttgagtttt gagcaaatgg agtgggcgag
     2221 ctgcgagtat tacagccatc acggtaaaat gatgccggac gactggcatg agcaacttag
     2281 ccgtttcgac gccatctatt ttggtgccgt cggctggccg gacaccgttc cggaccatat
     2341 ttcgttgtgg ggttcgctgc tgaaatttcg tcgtgaattc gaccagtacg tcaacctgcg
     2401 cccggttcgt ctctttcctg gcattccctg cccgctggcg ggaaaacagc ctggcgacat
     2461 cgatttttac gtggtcaggg aaaacaccga aggcgaatat tcctcgctcg gcggtagagt
     2521 gaatgaaggt acagagcatg aagtcgtcat tcaggaatcg gtatttacgc gtcgtggtgt
     2581 cgatcgcatt ttgcgttatg ccttcgaact tgcgcaaagc cgcccacgta agacgctaac
     2641 ttctgccact aaatcaaacg gtttagccat cagcatgccg tactgggatg agcgagtgga
     2701 agcaatggcc gagaattacc cggagatccg ctgggacaag cagcatattg atattctctg
     2761 cgcgcgtttt gtgatgcagc cggaacgctt cgatgtggtg gtggcgtcca atttgtttgg
     2821 cgatatcctt tccgatcttg gcccggcctg caccggcacc attggcattg ccccatccgc
     2881 caacctgaat ccggaacgca ctttcccgtc gctcttcgag cctgtccacg gttccgcgcc
     2941 ggatatctac gggaaaaata ttgctaaccc tatcgccaca atttgggccg gtgcaatgat
     3001 gctcgatttt ctcggcaatg gcgatgagcg tttccagcaa gcgcataacg gtattctggc
     3061 agcgattgaa gaagtgattg ctcacgggcc gaaaacaccg gatatgaaag gcagtgccac
     3121 cacgccacag gttgccgacg cgatttgcaa aattattttg cgttaaggtc aaaccagttt
     3181 atttgaaccg cgtcactgac gcggtttttt tcttcgttct ttgcagttaa taacctgcgt
     3241 catttcacct tttattgttt ccgtttcgtg ttttatggct ttccgtattc ttaattgttt
     3301 aatttatgta acatgcaaat aatcaatttg ttaagcacaa catacaactc attaccctgc
     3361 aaataattac aactggcaaa agccagagac gggcttttat gcccaggaga tgactcatga
     3421 tgagcaatgt taagaaaaaa gatgtgccgc tgataagcat cagcctggtg gccattcttt
     3481 tcatcgcagc tgcattaagc cttttcccac aacaatcggc cgacgcggcc aacgcaatat
     3541 acacttttgt tacgcgtacg ttaggttccg ccgtacaggt attggttttg ctggcaatgg
     3601 gactggtgat ttatttagcc accagtaaat acggcaatat tcgtcttggc gaaggaaaac
     3661 cggaatacag cacgctctcc tggctgttta tgtttatttg tgccggttta ggttcttcta
     3721 cgctttattg gggggttgct gaatgggcct attattatca aacgcctgga ttaaatatcg
     3781 caccgcgttc acaacaggca ctcgaattta gcgtccctta ctccttcttc cactggggca
     3841 tcagcgcttg ggcaacttat acgctggcct cattaatcat ggcttatcac tttcatgtgc
     3901 gtaaaaacaa aggtctgagt ctttccggca ttattgccgc gattaccggc gttcgcccgc
     3961 aaggcccatg gggaaaactg gttgatttga tgttcctgat cgccactgtc ggcgcactga
     4021 ctatttccct tgttgtcacc gcagcaacct ttactcgtgg actttccgca ctgaccggtt
     4081 tacccgataa cttcaccgtg caggcatttg tgatcctgct ttccggcggc attttttgcc
     4141 taagctcatg gattggtatc aacaacggtt tgcaacgtct gagcaaaatg gttggctggg
     4201 gcgcgttcct gctgccatta ctggtgctga ttgtcggtcc aaccgaattt attaccaaca
     4261 gcatcatcaa tgccatcggc ctgaccacgc aaaacttcct gcaaatgagc ttattcaccg
     4321 atccgcttgg cgatggttca tttacccgca actggaccgt tttctactgg ctgtggtgga
     4381 tctcatacac ccctggcgta gcaatgtttg tcacccgcgt ttcccgcggt cgtaagatta
     4441 aagaagttat ctggggactg atcctcggca gcaccgtcgg ttgctggttc ttcttcggcg
     4501 taatggaaag ctatgccatt catcagttta tcaatggcgt aatcaacgtc ccacaggtgc
     4561 tggaaacact gggcggcgaa acagctgtac agcaagttct gatgtcgttg ccagccggta
     4621 aattgttcct cgccgcatac ctgggcgtga tgattatttt ccttgcctcg catatggatg
     4681 cagtggccta caccatggcg gcgaccagta cgcgtaatct ccaggaaggt gacgatcctg
     4741 accgtgggct gcgtcttttc tggtgcgtgg tgatcactct gatcccgctt tccatcttgt
     4801 ttaccggtgc ttcgctggaa acgatgaaaa ccaccgtcgt gctcacagcc cttcccttcc
     4861 tcgtcatttt actggtgaaa gtcggcggat ttattcgctg gctgaaacag gattacgccg
     4921 acattccggc tcatcaagtt gaacattatc tcccgcagac accggttgaa gccctggaaa
     4981 aaacaccagt gctccctgcg ggaaccgtat tcaaaggcga caactgagcg ccatcatcct
     5041 aacgataaag gtatccctat gagcaatctg agccctgact tcgtactacc cgaaaatttt
     5101 tgcgctaacc cgcaagaggc gtggaccatt cctgcccgtt tttataccga tcagaacgcc
     5161 tttgaacacg aaaaagagaa cgtcttcgcc aaaagctgga tttgcgtcgc tcacagcagc
     5221 gaactggcga atgccaatga ttatgtgacg cgtgagatca ttggcgaaag catcgtgctg
     5281 gtacgcggtc gtgataaggt tttgcgcgcg ttctataacg tgtgtccgca ccgtggtcat
     5341 cagttgttga gcggtgaagg aaaagcaaaa aatgtgatta cctgcccata tcacgcatgg
     5401 gcattcaaac tcgatggcaa tctggcccat gcacgtaact gcgaaaacgt cgccaatttc
     5461 gatagcgaca aagcgcaact ggttccggtg cgtctggaag aatatgccgg attcgtcttc
     5521 atcaacatgg accccaacgc caccagcgta gaagatcagt tacccggtct gggcgcgaaa
     5581 gtgctggaag cctgcccgga agtccacgat ctgaaactgg cggcccgctt taccacccgc
     5641 acgcctgcca actggaagaa cattgtcgat aactatctcg agtgctatca ctgtggtccg
     5701 gcgcatccag gtttctccga ctccgtacag gttgatcgtt actggcacac catgcacggt
     5761 aactggacgc tgcaatacgg tttcgccaaa ccgtccgaac agtcgtttaa atttgaagag
     5821 ggtacggatg cggcattcca cggtttctgg ctgtggccgt gcacgatgct gaatgtcacc
     5881 ccgatcaaag ggatgatgac ggtcatttat gaattcccgg tggattctga aactaccctg
     5941 caaaactacg atatttactt caccaatgaa gagttaaccg acgagcaaaa atcgctgatt
     6001 gagtggtatc gcgatgtgtt ccgtccggaa gatttacgtc tggttgaaag cgtacagaaa
     6061 gggctgaaat cgcgtggcta tcgtggtcag gggcgcatca tggccgacag tagcggtagc
     6121 ggcatttccg aacatggtat cgcccatttc cataatctgc tggcgcaggt atttaaggac
     6181 taatgacatc ggcggcggta ttttccgccg ccgggctgat ttttgatgga gtacagcaat
     6241 gtcagactat caaatgtttg aagtacaggt gagtcaggtg gaaccgctta ccgaacaggt
     6301 gaaacgcttc acgctggtgg caaccgatgg caaaccgctg cctgcgttca ccggaggaag
     6361 tcacatcata gtgcagatga gcgatggtga taaccagtac agcaatgcgt attcactact
     6421 gagttcaccg cataacacct ctagttatca gattgccgtt cgactggaag aaaactcgcg
     6481 cggcggttcc cgctttctgc atcagcaggt aaaagtgggc gatcggttaa cgatctcaac
     6541 gcctaataac ctgtttgcgc taatttcctc agccagaaag catctgttta tcgcgggcgg
     6601 tattggtatc acccctttcc tgtcgcacat ggcagagttg caacacagcg acgtcgactg
     6661 gcagctacat tactgctcgc gaaatccaga aagttgcgca tttcgtgatg agctagtcca
     6721 gcatccgcag gctgagaaag tccatttgca tcattcatca accggaacac gactggaatt
     6781 agcgcgatta ttggcggata tcgaacctgg cacacacgtt tatacctgtg gccccgaggc
     6841 gctaattgaa gcggtaagaa gtgaagctgc gcgtctggac atcgctgccg atacgctgca
     6901 ctttgagcaa tttgctatcg aagacaaaac cggcgatgca tttaccctgg tgcttgcccg
     6961 ttccggaaaa gagtttgtgg tgccggaaga gatgactatt ttgcaggtta ttgaaaataa
     7021 taaagccgcg aaagtggaat gtttatgtcg tgaaggggta tgcggaacct gcgaaacggc
     7081 aatactggaa ggtgaagctg accatcggga tcagtatttt agcgatgaag agcgtgccag
     7141 ccagcaaagt atgttgatct gttgttcgcg tgcgaagggt aaacgcctgg tgttggattt
     7201 gtagtttgcc gaagccggat gtggcgctga gcgcgcccag tccggcttcg gaaaatttta
     7261 ctgcggatat tcctgcaata aattgtgtaa tgcttccgcc atcagctcac cacgccagcc
     7321 ggaaatcagc tccggcaaat tgttctgcgg tttcagtttc cagtgccagt tcagcagttg
     7381 gttgatttgc cgacgcgatg ccagcaattc ggcgctgata ttatgcgttt cgctcacgtc
     7441 agtaatcagc gacttaatcg ctttaaacgc tttacgataa cccggcatgt ccatcaggtt
     7501 aagcatcggc tgcggtaagg catcttccgg caatgtctgc gctttttcca ccagcgccag
     7561 cagcgtttta ccgtgaaagc ggatttcgct accggataaa cccaggctgt ccagttcgcc
     7621 taaacttccc ggcatataac gcgctaccga ccacaaatgc tcttcacgca cgacaaagtt
     7681 caccgccaga tcgcgctctc gcgccttgcg cagtcgccag tcggctaaca gttgcagaca
     7741 ggccagttgg cgtgtgcgta attgccaggc attggtgata tcacgccagg catcttccgg
     7801 cgcaacgact tcctgacgac gcatttgcat caggcgacat tcatccagcg ccgctggtag
     7861 ccagccggag gcctccgttt ctaccataag cttggcggtg atcggtaaca gataccagac
     7921 atccgccgct gcatattcac actgacgttc ggtcagcggt ctggccagcc agtcggtgcg
     7981 cgattcactc ttgtccagcg taacgccgga atactcttcc accatggaag cgaaccccca
     8041 tgacatcggg cgtccgcaga aggcagcaag gatttgcgtg tcaatcaggg gttgtggtaa
     8101 ttcgccaaag acattgagga acacttccag atcttcactg cctgcatgga gaaattttgt
     8161 gatggacgga tcgcgcagga tcgctttcag cggtgaccag tcggtgatcc cgagaggatc
     8221 aattagcgcc agatgctcgc catcgaaaag ttgaatcaac cccagctgcg ggtaataagt
     8281 gcgcgtacga acaaattcag tatccagggc tatcgccgga aaggcacgga cggcttcaca
     8341 caaagaagcc agcgcatcgt ccgtggtaat catttggtaa ttcaaattgt attctcttta
     8401 gtgggcgtca aaaaaaacgc cggattaacc ggcgtctgac gactgactta acgctcaggc
     8461 tttattgtcc actttgccgc gcgcttcgtc acgtaattct cgtcgcaaaa tttttccgac
     8521 gttagatttc ggtaactcat cacgaaactc caccagcttc ggtactttgt agcccgtgag
     8581 ctgacggcgg caaaaagtca ccagtgactc ttcggtaagc gatggatctt ttttcactac
     8641 aaagattttt accgcttcac cactggagcc ggaaggtacg ccaacagccg cgacttcctg
     8701 tacgccagga tgctgcatga cgacatcttc aatctcgttt ggatagacgt taaaaccgga
     8761 aaccagaatc atgtcttttt tacgatcgac aatgcgcagg aacccttctt catccatcac
     8821 cgcgatgtcg ccggtgtgta accagccatt tttgatgatc tcatcggtag catccggacg
     8881 ctgccagtaa cccagcatca cctgcggtcc tttgacacaa agctcacccg gttgacccgg
     8941 tggtacttca ttatcatcat catccaccag tttggcttcc gtcgacggca ccggcaaacc
     9001 gatgctacca ctatgataat caatatcata tgggttaacg ctgaccagcg gcgcacactc
     9061 ggtaaggcca tagccttcca gcagatactg tcctgtcagt ttcacccaac gctctgccac
     9121 cacttgctgc actggcatcc caccgcctgc ggaaagatgc agactggaga aatccagctg
     9181 ctggaactct ttattgttca gcaacgcatt gaacaaggtg ttaacgccag tgatagcggt
     9241 aaacggatat ttcgctaact cttttaccaa ccctggaata tcacgcgggt tagtgataag
     9301 caggttctgc ccacccagtt cgataaacag caggcagtta atggtcaggg caaaaatgtg
     9361 atacagcggc agtgccgtta ctaccagttc tttacctgga tgcagcaacg gcccataggt
     9421 ggcgttgacc tgttccaggt tcgccagcat attgcggtga gtcagcatcg cgcctttcgc
     9481 cacacctgtg gtgccgccgg tgtattgcag gaaggctaaa tcttccggca ccagttcggg
     9541 tttgacgtac tgcatccggt agccgttatg cagtgcgcta cgaaatgaaa tggcatctgg
     9601 cagatggtat ttcggcacca aacgcttgat gtatttaaca acgaaattga ctaccgtgcc
     9661 ttttgccgta gatagctgat cgcccatacg ggtcagaatt acgtgctgaa cggcggtttt
     9721 atcaaccact ttttccagtg tgtgagcaaa gttagacacg ataacaatcg ccgatgcgcc
     9781 gctatcgtta agctgatgct caagctcacg cggggtatac aacgggttaa cgtttacgac
     9841 gatcatcccg gcacgcaaaa tgccaaacag cgccaccgga tattgcaata aattaggcat
     9901 catcaacgca acgcgatcgc ctttcttcag ccccaaccct tgttgcaaat aagcggcaaa
     9961 cgcgcgactg cgttcttcca gcttgcggaa ggtcattacc tcccccatat tcacaaacgc
    10021 aggctgatcg gcgtagcgcg cgaccgactg ctcaaacata tctaccagag attgataacg
    10081 gtcagggttg atctccgtcg gaacgtccgc gggataacgg ttaagccaaa ccttcttcaa
    10141 ttcttcacct ctaaaatgcg tgttcgtcgt catcgcaacc ccaaatgata tacatgccgt
    10201 taacataata ttaactcatc ataccagctt gataattacc caac
//
LOCUS       DAZUCE010000185        10214 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZUCE010000185 DAZUCE010000000
VERSION     DAZUCE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.185"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            46..621
                     /gene="ssuE"
                     /locus_tag="ACHY03_003878"
     CDS             46..621
                     /gene="ssuE"
                     /locus_tag="ACHY03_003878"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFS3907056.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALHTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            614..1573
                     /gene="ssuA"
                     /locus_tag="ACHY03_003879"
     CDS             614..1573
                     /gene="ssuA"
                     /locus_tag="ACHY03_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFS3907057.1"
                     /translation="MRNIIKLALVGLLSVSTLAVAAESSPEALRIGYQKSNISSVLAK
                     NYQMLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1570..2715
                     /gene="ssuD"
                     /locus_tag="ACHY03_003880"
     CDS             1570..2715
                     /gene="ssuD"
                     /locus_tag="ACHY03_003880"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFS3907058.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSHPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVNLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2727..3518
                     /gene="ssuC"
                     /locus_tag="ACHY03_003881"
     CDS             2727..3518
                     /gene="ssuC"
                     /locus_tag="ACHY03_003881"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFS3907059.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3515..4282
                     /gene="ssuB"
                     /locus_tag="ACHY03_003882"
     CDS             3515..4282
                     /gene="ssuB"
                     /locus_tag="ACHY03_003882"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFS3907060.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGESETRLRKQG"
     gene            complement(4325..6937)
                     /gene="pepN"
                     /locus_tag="ACHY03_003883"
     CDS             complement(4325..6937)
                     /gene="pepN"
                     /locus_tag="ACHY03_003883"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFS3907061.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDLQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7203..8405
                     /gene="pncB"
                     /locus_tag="ACHY03_003884"
     CDS             7203..8405
                     /gene="pncB"
                     /locus_tag="ACHY03_003884"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFS3907062.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQANVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAITHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8575..9975
                     /gene="asnS"
                     /locus_tag="ACHY03_003885"
     CDS             8575..9975
                     /gene="asnS"
                     /locus_tag="ACHY03_003885"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFS3907063.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2285 a         2644 c         2768 g         2517 t
ORIGIN      
        1 tatcaatgtt aacaaaacaa gaacaattgg ttataaggag agagtatgcg tgtcatcacc
       61 ctggcgggta gtcctcgctt tccttctcgc tccagctcct tgctggaata tgcgcgggaa
      121 aaactaaatg gcctggatgt agaggtttat cactggaatc tgcaaaactt cgccgcggaa
      181 gatctgcttt atgctcgttt cgatagtccg gcactccata ccttcaccga acagttgcaa
      241 caggctgacg ggctgattgt cgccacgcct gtgtataaag ccgcttattc cggtgcgctg
      301 aaaaccctgc tcgacctgct gccagaacgt gcgttgcaag gcaaagtggt gctaccgctg
      361 gcgacgggcg gtacggtggc ccatctgctg gcggtcgatt atgcccttaa gccagtttta
      421 agtgcgctga aagcccagga gatcctgcac ggcgtgtttg ccgatgactc acaagtaatt
      481 gattaccatc acaaacccca gttcacgccg aatttacaaa cccgtcttga taccgcgcta
      541 gaaactttct ggcaggcatt gcaccgccgc gatgttcagg ttcctgatct tcattctctg
      601 cgaggtaatg cccatgcgta acatcattaa actggcgctg gtgggattgc ttagcgtctc
      661 tacactcgcg gttgccgcag agtcttcgcc agaggcgtta aggattggct atcagaaaag
      721 caacatcagt tcggtgctgg cgaaaaatta ccaaatgctg gaaaaacgct atccgcaaac
      781 aaaaatctcg tgggtggagt tccccgctgg cccgcaaatg cttgaggcgt taaacgttgg
      841 cagtattgat ctcggcagta ccggggatat tccgccaatc ttcgcccagg ctgccggggc
      901 tgatttgctg tacgtgggcg tcgagccgcc gaagccaaaa gccgaggtga ttctggtggc
      961 agaaaacagc ccgatcaaaa ccgtagccga tcttaaaggc cacaaagttg cgttccagaa
     1021 aggttccagt tcacacaatc ttttactgcg cgcactacgc caggccggac ttaagtttac
     1081 tgacatccag cccacttatc tgacgccagc tgatgcccgc gccgcgttcc agcaaggtaa
     1141 cgttgacgcc tgggctatct gggatcccta ctactccgct gcattattac agggcggcgt
     1201 gcgggtgttg aaagacggca ccgatctcaa tcaaaccgga tcgttttatc tggcagctcg
     1261 cccctatgca gaaaaaaacg gcgcttttat tcagggtgta ctggcaacct ttagtgaggc
     1321 cgatgcgtta acccgcagcc agcgcgagca aagcatcgct ttactggcaa aaacgatggg
     1381 cttaccggca ccggtgattg cctcttactt agatcatcgc cctcctacca ccatcaaacc
     1441 ggttaacgcc gaggttgccg ccttacagca gcaaacggca gatctgtttt atgaaaaccg
     1501 tctggtgccg aaaaaagtcg atattcgcca acgcatctgg cagcccactc aactggaagg
     1561 aaaacaatta tgagtctgaa tatgttctgg tttttaccga cccatggtga cgggcattat
     1621 ctgggaacgg aagaaggttc acacccggtt gatcacggtt atctgcaaca aattgcgcaa
     1681 gcggcggatc gtcttggcta taccggtgtg ctgatcccaa cggggcgctc ctgcgaagat
     1741 gcgtggctgg ttgccgcatc gatgatcccg gtgacgcagc ggctgaagtt tcttgtcgcc
     1801 ctgcgtccca gcgtaacctc acctaccgtt gccgcccgcc aggccgccac gcttgaccgt
     1861 ctctcaaatg gacgtgcgtt gtttaacctg gtcacaggca gcgatccaca agaactggca
     1921 ggcgacggcg tgttccttga tcatagtgag cgctacgaag cctcggcgga atttacccag
     1981 gtctggcggc gcttattgct gggggaaacc gtcgatttca acggtaaaca tattcatgtg
     2041 cggggagcca aactgctctt cccgccgatt caacagccgt atcctccgct ttactttggc
     2101 ggatcgtcag acgtcgccca ggagctggcg gcagaacagg ttaatctcta cctcacctgg
     2161 ggcgaaccgc cggaactggt taaagagaaa atcgaacaag tgcgggcgaa agctgccgcg
     2221 catggacgca aaattcgttt cggcattcgt ctgcatgtga ttgttcgtga aactaacgac
     2281 gaagcgtggc aggccgccga gcggttaatc tcgcatcttg atgatgagac tatcgccaaa
     2341 gcacaagccg cattcgcccg cacggattcc gtggggcaac agcgaatggc agcgctacat
     2401 aacggcaagc gcgacaatct ggagatcagc cccaatttat gggcgggcgt tggcttagtg
     2461 cgcggcggtg ccgggacggc gctggtgggc gatggtccta cagtcgctgc gcgaatcaac
     2521 gaatatgccg cgcttggcat cgacagcttt gtgctttcgg gctatccgca tctggaagaa
     2581 gcgtatcggg tcggcgagtt gctgttccca catctggatg tcgccattcc ggaaattccc
     2641 cagccgcagc cgctgaatcc gcaaggcgaa gcggtggcga atgattttat cccccgtaaa
     2701 gtcgcgcaaa gctaaggaac acgatgatgg caacgccagt gaagaagtgg ttattgcgcg
     2761 ttgccccctg gtttttaccg gtgggcatcg tggcggtgtg gcaactggcc tcctcggttg
     2821 gctggctgtc gacgcgtatt ttgccttcac cggaaggagt ggtgacggcg ttctggacgc
     2881 tctccgccag cggcgaactg tggcagcatc tggcgatcag ctcctggcgg gcgctgattg
     2941 gcttttcaat tggcggatcg ctggggttga ttttgggact gattagcggg ctgtcacgct
     3001 ggggtgagcg actgctggat acctcaattc agatgttgcg caacgtgccg catctggcgc
     3061 tgatcccgct ggtgattttg tggtttggca ttgatgaatc cgcaaaaatc tttctggtgg
     3121 cgctcggtac gctattcccc atttatatca acacctggca tggcatccgt aatatcgatc
     3181 gcgggttggt ggagatggcg cgtagctatg gattatccgg cataccgctg tttatccatg
     3241 tgatcctgcc tggtgccctg ccctcaatta tggtcggcgt gcgttttgcg ttgggcctga
     3301 tgtggctgac gctgattgtt gccgaaacca tttctgccaa ttcaggcatt ggttatctgg
     3361 cgatgaatgc gcgggagttt ttgcaaacgg acgtggtggt ggtcgccatt attctttacg
     3421 ccctgctcgg caaactggct gacgtcagcg cgcagctgtt agagcgcctc tggttgcgct
     3481 ggaacccggc ttatcatttg aaggaggcca cggtatgaat actgctcgtc tgaaccaggg
     3541 cacgccattg ttgctcaatg cagtaagcaa acattacgcg gaaaatatcg tcctgaacca
     3601 actggattta catattcctg caggtcagtt tgtggcggtg gtgggccgca gcggtggtgg
     3661 caaaagtacc ctgctgcgcc tgctggcagg tctggaaacg ccaaccgctg gcgatgtgtt
     3721 agcaggcacc acaccgctgg ctgaaattca ggaagacacg cgaatgatgt ttcaggatgc
     3781 gcgtctgcta ccgtggaaat cggtgattga taacgttggg ttaggcctta aaggccagtg
     3841 gcgcgatgcc gcgcgtcggg cgttggctgc ggtaggactg gagaatcgcg ccggggaatg
     3901 gcctgccgca ctttcaggcg ggcagaaaca gcgagtggcg ctggcaagag cgttgattca
     3961 tcgaccgggt ttattgttgc ttgatgaacc gctcggtgcg ctggatgcct taacgcgact
     4021 cgagatgcag gatttgattg tgtcactttg gcaggagcac ggctttaccg tactgttggt
     4081 gacgcatgat gtgagcgaag cggtagcaat ggctgaccgg gtgttgttaa ttgaagaggg
     4141 aaaaattggt ctggatttga cggtggatat cccccgtccg cgccggttag ggtcggtgcg
     4201 attagcggag ctggaagcgg aagtgttgca acgggtaatg cagcgtggtg agtctgaaac
     4261 tcgcctgaga aaacaggggt aaaatccccg aatggcggcg ctattgccgc cattcggtta
     4321 tttattaagc cagtgctttg gtgatcttct cgtacagatc gccagagaga ttttccagcc
     4381 ctttcaactg ttccagcgcc gcgcgcattt tctcctgacg tttagcatca taacgtttca
     4441 ggcgaatcag cggttcaatc aggcgtgaag ctacctgcgg gttacggctg ttgaggtcgg
     4501 taagcatttc caccaggaac tggtaaccgc tcccatcttc ggcatggaac gctgccgggt
     4561 tgctgcccgc aaacgcacca atcaacgaac gaatacggtt cgggttgctc atggtaaatg
     4621 agcgatgctg caacaggccg cgcaccgtct ccagcacatt cgccgccggg ctggtggctt
     4681 gcaggataaa ccatttatcc atcaccagac cgtcctgatg ccacttgtcg tcgtactcct
     4741 gcatcagtgc gtcacggcat ggcagctgtg cggcaaccgc cgcggaaagc gccgccagcg
     4801 catcggtcat attgtttgct tcgtggaact gcttgctcac cagcacatca gccagatgcg
     4861 tttcaccaaa agcaaggaag cgcaggcagg cattacgcag agtgcgtttt gcaatatctt
     4921 catgctcaac gcggtactcg ctctggtaat tcgcgttgta aatagccagc agctcatccg
     4981 ccagttcagt cgccagagta cgagtgagtg cttcgcgtac ttcggcaata gcaatcgggt
     5041 cgatgatatc gaacagttca gccatttcat tgacggatgg cagagtcaga atttctgccg
     5101 ccaatgccgg gtcaatcttc tcatcaagca gcaccgcgcg gaaggcgtcg gccacatgca
     5161 ccggcagaga cagcggctgc ccttgctgat gacgggcgac gttcagcttg atgtaggttg
     5221 ccagcaaact ttgcgccgca tcccaacggg agaaatcatt acgcgcatga cgcatcagga
     5281 aggtcagttg cagatcgctc cacttatatt ccagtttcac tggcgcagag aattcgcaca
     5341 gcagcgcagg caccggctgg aagtagacat tatcaaagac aaaggtctgt tccgcctgag
     5401 tgacgttcag cacggaattc accggatgac cgcctttctg caacgggatc actttgcctt
     5461 cgttatcata cagttcgatg gcaaacggaa tatgcagcgg ctgtttttct gcctgatccg
     5521 gcgtggctgg cgtgcgctgg ctgatggtca gggtgtactg ctcggtttcc ggattgtagt
     5581 cgtctttgac ggtcacaacc ggcgtaccgg actggctgta ccaacggcgg aaatgggaga
     5641 gatcgacatt cgacgcatct tccatcgcct gcacaaaatc gtcacaggtc gctgcgctac
     5701 catcatgacg ctcgaaatag agctgcatcc ctttctggaa attttcttcg ccaagcaggg
     5761 tgtggatcat gcgaatcacc tccgcgccct tctcgtaaac ggtcagggtg tagaagttgt
     5821 tcatctcaat gaccatatcc gggcggattg ggtgcgccat cgggctggcg tcttccgcaa
     5881 actgcaatcc gcgcatggtg cgcacattat tgatacggtt taccgcgcgg gaaccaaggt
     5941 cagagctgaa ttcctgatcg cggaagacgg ttaaaccttc tttcaggctg agctggaacc
     6001 agtcgcggca ggtcactcgg ttaccggtcc agttatggaa atattcatgg ccgataacgc
     6061 gttcaatatc gaggtaatct ttgtcggtgg cggtatcggt gcgggccagc acatatttgg
     6121 agttaaagat attcagcccc ttattctcca ttgcgcccat attgaagaaa tccaccgcga
     6181 cgatcatata gatgtcgagg tcatactcca ggccgaagcg ttcttcatcc catttcattg
     6241 agtttttcag cgaggtcatc gcccacggcg cgcgatcaag gttaccgcga tcgacgtaca
     6301 gctccagcgc tacttcgcga ccagaacgcg tggtgaaggt gtcgcgcagt acatcaaagt
     6361 cgcctgccac cagcgcaaac aggtagcacg gtttcgggaa cgggtcctgc cactgtaccc
     6421 aatgacgtcc gttttccagt tcgccttgcg caacgcggtt accgttggaa agcaggaaag
     6481 gatatttggt tttatcggca ataattttgg tggtaaaacg cgccagcacg tccgggcggt
     6541 cgagataata cgtaatatgg cggaaacctt cggcttcaca ctgggtgcaa agcgcatcgc
     6601 ctgactgata aagcccttcc agcgccgtat tcgccgccgg gcttatttca ttaatgatct
     6661 taagcgtaaa acgctccggc aaattactga tgaccagtgc gccctcttct tctttccagg
     6721 cggtccacgg ctcatcatta atatgaacag aaaccagttt gaggtcttcg ccgttgagac
     6781 gaagcggagc atctgatgca ccatgacgga cagcctggct gaccgcggta acgaccgtct
     6841 tttgcgcgtc gaggtcaaag gtcaagtcaa tatcagtaat ctggtaatcc ggcgcacgat
     6901 aatcgtgacg gtatttggct tgtggctgtt gagtcataaa taacctttag catcttttat
     6961 agagtctggt gttcagtcta ttcctgttgc gtaaatcgcg ctatgcagaa tcttcatctt
     7021 ttcaggtaca aacgccttta ttgctacatt tttataacat acaccgcgta atgccatcga
     7081 ccagaaaggt ggcatatggt gtgatcgggg ttcaataaat tgcgaaacaa ggtatactcc
     7141 agcagttcct gaagatgttt attgtactaa acgctcctgt acgaggacgc tactgcgcac
     7201 ctatgacaca attcgcttct cctgttctgc actcgttgct ggatacagat gcttataagt
     7261 tgcatatgca gcaagccgtg tttcatcact attacgatgt gcatgtcgcg gcggagtttc
     7321 gttgccgagg tgacgatctg ctgggtattt atgccgatgc tattcgtgaa caggttcagg
     7381 cgatgcagca cctgcgcctg caggatgatg aatatcagtg gctttctgcc ctgcctttct
     7441 ttaaggccga ctatcttaac tggctacgcg agttccgctt taacccggaa caagtcaccg
     7501 tgtccaacga taatggcaag ctggatattc gtttaagcgg cccgtggcgt gaagtcatcc
     7561 tctgggaagt tcctttgctg gcggttatca gtgaaatggt acatcgctat cgctcaccgc
     7621 aggccaacgt tgcgcaagcc ctcgacacgc tggaaagcaa attagtcgac ttctcggcgt
     7681 taaccgccgg tcttgatatg tcgcgcttcc atctgatgga ttttggcacc cgccgccgtt
     7741 tttctcgcga agtacaagaa accatcgtta agcgtctgca acaggaatcc tggttcgtgg
     7801 gcaccagcaa ctacgatctg gcgcgtcggc tttccctcac gccgatggga acacaggcac
     7861 acgaatggtt ccaggcacat cagcaaatca gcccggatct agccaacagc cagcgagctg
     7921 cacttgctgc ctggctggaa gagtatcccg accaacttgg cattgcatta accgactgca
     7981 tcactatgga tgctttcctg cgtgatttcg gtgtcgagtt cgctagtcgg tatcagggcc
     8041 tgcgtcatga ctctggcgac ccggttgaat ggggtgaaaa agccattaca cattatgaaa
     8101 agctgggaat tgatccacag agtaaaacgc tggttttctc tgacaatctg gatttacgca
     8161 aagcggttga gctataccgc cacttctctt cccgcgtgca attaagtttt ggtattggga
     8221 ctcgcctgac ctgcgatatc ccccaggtaa aacccctgaa tattgtgatt aagttggtag
     8281 agtgtaacgg taaaccggtg gcgaaacttt ctgacagccc tggcaaaact atctgccatg
     8341 ataaagcgtt tgttcgggcg ctgcgcaaag cgttcgacct tccgcatatt aaaaaagcca
     8401 gttaatatca tcagggagct aatcggctcc ctttttttac ctttaattcc gaaatctttc
     8461 gctgcatttg cgaattctgc ttgtctgatt gcagatgcca ggtaacatag gtatcccccc
     8521 catttacggg tggacaagtg tttatttttt ccgactatta acagagagaa tattatgagc
     8581 gttgtgcctg tagccgacgt actccagggc cgcgtagccg ttgacagcga agtcaccgta
     8641 cgcggatggg tacgtacccg ccgagattca aaagctggca tctccttcct cgccgtttat
     8701 gacggttcct gctttgatcc tgtacaggct gtcatcaata attctctgcc caattacaat
     8761 gaagacgtcc tgcgtctgac caccggttgc tcggtcattg tgacgggtaa agtcgtggcg
     8821 tcgccgggcc aggggcaaca atttgaaatt caggccagca aggttgaagt tgctggttgg
     8881 gttgaagatc cagacactta cccgatggcg gcaaaacgcc acagcattga gtatctgcgt
     8941 gaagtcgctc acctgcgtcc gcgcacaaac ctgattggtg ccgtcgcgcg cgttcgccat
     9001 acgctggcgc aggcgctgca tcgcttcttt aacgagcagg gattcttctg ggtttcaacg
     9061 ccactgatta ctgcgtctga taccgaaggg gctggcgaaa tgttccgcgt ttctacgctg
     9121 gatctggaaa atctgccgcg taacgatcag ggcaaagtgg atttcgacaa agacttcttt
     9181 ggtaaagagt ctttcctgac cgtatctggc cagttgaacg gcgaaaccta cgcttgcgca
     9241 ttatccaaaa tttatacctt cggcccaact ttccgtgctg aaaactccaa caccagccgt
     9301 cacctggcgg aattctggat gctggagccg gaagtggcgt ttgctaacct gaacgatatc
     9361 gcaggtctgg ctgaagccat gctgaaatat gtcttcaaag cggttctcga agaacgcgct
     9421 gacgacatga aattcttcgc tgaacgcgta gataaagatg ccgtttcacg tctggaacgc
     9481 ttcattgaag ccgattttgc gcaggtggat tacaccgacg cagtgaccat tctcgaaaac
     9541 tgcggcagga agtttgaaaa cccggtttac tggggcgttg atctctcttc tgagcatgag
     9601 cgttatctgg cggaagaaca ctttaaagca ccggtagtgg ttaaaaacta tccgaaagat
     9661 attaaagcgt tctatatgcg ccttaacgaa gacggtaaaa ccgttgcggc tatggacgtt
     9721 ctggctccgg gcatcggtga gatcattggt ggctcccagc gtgaagaacg tctggacgtg
     9781 ctggacgagc gtatgctgga aatgggcctg aataaagaag attactggtg gtatcgcgat
     9841 ctgcgtcgct acggtactgt tccgcattca ggtttcggtc ttggttttga acgcctgatt
     9901 gcttatgtaa ctggtgtgca aaacgtgcgt gatgtgattc cgttcccacg tactccgcgt
     9961 aacgccagct tctaatttag cgtctacaag agccagcacc cgctggctct tttttatcct
    10021 ttctgtcaag ttatctgttt gttaagtcaa tcaatctatt tgcaaccccg ccataacgat
    10081 catcctgtta cggaatatta cattgcaaca tttatgcaca aaaaataacc cgcattctta
    10141 ttgcggatta gttttttctt agctgatagc acaattttca tactattttt tggcattctg
    10201 gatgtctgaa agag
//
LOCUS       DAZUCE010000186        10089 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZUCE010000186 DAZUCE010000000
VERSION     DAZUCE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.186"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            419..700
                     /gene="drpB"
                     /locus_tag="ACHY03_003886"
     CDS             419..700
                     /gene="drpB"
                     /locus_tag="ACHY03_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFS3907064.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVISRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            740..1435
                     /gene="yedJ"
                     /locus_tag="ACHY03_003887"
     CDS             740..1435
                     /gene="yedJ"
                     /locus_tag="ACHY03_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFS3907065.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1502..2920
                     /gene="dcm"
                     /locus_tag="ACHY03_003888"
     CDS             1502..2920
                     /gene="dcm"
                     /locus_tag="ACHY03_003888"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFS3907066.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISGCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2901..3371
                     /gene="vsr"
                     /locus_tag="ACHY03_003889"
     CDS             2901..3371
                     /gene="vsr"
                     /locus_tag="ACHY03_003889"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFS3907067.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFHVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3360..4280)
                     /gene="yedA"
                     /locus_tag="ACHY03_003890"
     CDS             complement(3360..4280)
                     /gene="yedA"
                     /locus_tag="ACHY03_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFS3907068.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLFNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4453..5370
                     /gene="yedI"
                     /locus_tag="ACHY03_003891"
     CDS             4453..5370
                     /gene="yedI"
                     /locus_tag="ACHY03_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFS3907069.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHREDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            5449..5631
                     /gene="yodC"
                     /locus_tag="ACHY03_003892"
     CDS             5449..5631
                     /gene="yodC"
                     /locus_tag="ACHY03_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFS3907070.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            5802..7496
                     /gene="dgcQ"
                     /locus_tag="ACHY03_003893"
     CDS             5802..7496
                     /gene="dgcQ"
                     /locus_tag="ACHY03_003893"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFS3907071.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHIVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELAMLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(7493..8308)
                     /gene="yedP"
                     /locus_tag="ACHY03_003894"
     CDS             complement(7493..8308)
                     /gene="yedP"
                     /locus_tag="ACHY03_003894"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFS3907072.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8607..8834)
                     /gene="yodD"
                     /locus_tag="ACHY03_003895"
     CDS             complement(8607..8834)
                     /gene="yodD"
                     /locus_tag="ACHY03_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFS3907073.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            8997..9185
                     /gene="dsrB"
                     /locus_tag="ACHY03_003896"
     CDS             8997..9185
                     /gene="dsrB"
                     /locus_tag="ACHY03_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFS3907074.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9229..9852)
                     /gene="rcsA"
                     /locus_tag="ACHY03_003897"
     CDS             complement(9229..9852)
                     /gene="rcsA"
                     /locus_tag="ACHY03_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFS3907075.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
BASE COUNT         2538 a         2403 c         2671 g         2477 t
ORIGIN      
        1 gttgaacgga aagataggga gtatcaatga ttgttgcgct aaacggctgg tgtgatcacg
       61 agcacattaa tgaaggcata aaaaaaagcc ggcatcatgc cggcccaaaa aatataaaca
      121 agacgataaa aggctaataa cggaggcatc atgacacaga aaaataaaaa ggtataattg
      181 tattcagtgc taatatattg attaaatgaa ttaataaagc cgatatcaaa gataaataaa
      241 atcgctcaaa acgatttttc ctgtattcac tgccgttgcg cataatttat ctatttgttc
      301 aaaaaatgat tgagtcttga ctggctcatt caatgtggaa aaatgtgact tttatcacat
      361 aatagtacta agtctgaatt ttccgggtta tctcaaaatg gaatacggtt cgacaaagat
      421 ggaagagaga ctctctcgca gccctggcgg caaactggct ttgtgggctt tctatacatg
      481 gtgtggctat ttcgtctggg caatggcgcg ttacatctgg gtgattagcc ggatccccga
      541 tgcccccgtg tcgggatttg aaagcgattt aggctctacc gcgggaaagt ggttaggggc
      601 actggtcgga tttttattta tggctctggt cggggcattg ttaggcagca ttgcctggta
      661 cacccgacct cgtcccgcac gtagcagacg ctatgaataa aaactgctaa ggtggcgact
      721 ctttggcaac ggagcgcgta tggacttaca acactggcag gcacagttcg aaaactggtt
      781 gaaaaaccat catcagcacc aggacgcggc ccatgacgtg tgtcattttc gccgcgtctg
      841 ggctacggca cagaagctgg cggcagatga cgatgtcgat atgctggtga ttttaacagc
      901 ctgttatttc cacgatattg tgagcctggc gaaaaatcat ccgcaacggc agcgttcttc
      961 aatcctggcg gcagaagaga cgcgtcgcct gctccgtgaa gagttcgtgc aatttccggc
     1021 ggagaaaatt gaggccgttt gtcatgccat tgccgctcac agtttcagcg cgcaaatcgc
     1081 ccccttaacc acggaagcta aaattgtcca ggatgcggat cggctggaag ccctgggggc
     1141 gattggcctg gcacgtgtgt ttgccgtttc aggggcattg ggcgtggcgc tgtttgatgc
     1201 cgaagatccg ctcgcacagc atcgcccgct tgatgataaa cgttacgcgc tggatcattt
     1261 ccagactaag ttgctgaagc taccgcaaac catgcaaacc gtaagaggca agcagctggc
     1321 gcagcacaac gcgcattttt tagtcgagtt tatggcgaag ctcagtgccg aactggcggg
     1381 ggagaatgaa ggtgtcgatc acaaggtgat agatgcgttt tcacccgcgg gctgagcgcg
     1441 tggccctaaa tggctgtaat tatgttaacc tgtcggccat ctcagatggc cggttaaatc
     1501 tatgcaggaa aatatatcag taaccgattc atacagcacc gggaatgccg cacaggcaat
     1561 gctggagaaa ctgctgcaaa tttatgatgt taaaacgctg gtggcgcagc ttaatggtgt
     1621 gggtgagaat cactggagcg cggcaatttt aaaacgtgcg ctggcgaatg actcggcatg
     1681 gcaccgttta agtgagaaag agttcgccca tctgcaaacg ttgttaccca aaccaccggc
     1741 acatcatccg cattatgcgt ttcgctttat cgatctattt gccggaattg gcggtatccg
     1801 tcgcggtttt gaatcgattg gcggacagtg cgtgtttacc agcgaatgga acaaacatgc
     1861 ggtacgcact tataaagcca accattattg cgacccggcg acgcatcatt ttaatgaaga
     1921 tatccgcgac atcaccctca gccataaaga aggcgtgagt gatgaggcgg cggcggaaca
     1981 tattcgtcaa cacattcctg aacacgatgt tttactggcc ggtttcccat gtcagccatt
     2041 ttcgctggct ggcgtatcga aaaagaactc gctcgggcgg gcgcacggtt ttgcctgcga
     2101 tactcagggc acgctgttct ttgatgtggt gcgcattatc gacgcgcgtc gtccggcgat
     2161 gtttgtgctc gaaaacgtca aaaacctgaa aagccacgac cagggtaaaa cgttccgcat
     2221 catcatgcag acgctggacg aactgggcta tgacgtggct gatgcagaag ataacgggcc
     2281 ggacgatccg aaaatcatcg atggtaaaca ttttctgccg cagcaccgtg aacgcatcgt
     2341 gctggtgggt tttcgtcgcg atcttaatct gaaagccgat tttactctgc gtgatatcag
     2401 cggatgtttc cctgcgcagc gagtgacgct ggcgcagctg ctggacccga tggtcgaggc
     2461 gaaatatatc ctgacgccgg tactgtggaa gtacctctat cgatatgcga aaaaacatca
     2521 ggcgcgcggt aacggcttcg gttatggaat ggtttatccg aacaatccgc aaagcgtcac
     2581 ccgtacgctg tctgcgcgtt attacaaaga tggcgcggaa attttaatcg atcgcggatg
     2641 ggatatggcc acgggtgaga aagactttga cgatccgctg aatcagcaac atcgtccacg
     2701 tcggttaacg cctcgggaat gcgcgcgctt aatgggtttt gaagcgccgg gagaagcgaa
     2761 attccgcatt ccggtttcgg acactcaggc ctatcgccag ttcggtaact cggtggtcgt
     2821 gccggtcttt gccgcggtgg caaaactgct tgagccaaaa atcaaacagg cggtggcgtt
     2881 gcgtcagcaa gaggcacaac atggccgacg ttcacgataa ggccactcgc agcaaaaata
     2941 tgcgcgcgat tgccacgcgt gatacggcga ttgagaagcg cctcgccagt ctgttaaccg
     3001 ggcagggcct ggcgtttcac gttcaggacg ccagtctgcc cggacgcccg gattttgtcg
     3061 ttgatgaata tcgctgcgtg atatttaccc atggctgctt ctggcatcat catcactgct
     3121 atctgtttaa agtgcctgcg actcgaaccg agttctggct ggagaagata ggtaaaaatg
     3181 ttgagcgcga tcgccgcgat atcagtcgct tgcaggaact cggctggcgc gtattgattg
     3241 tctgggagtg cgcgttacgt gggcgcgaga agctgacgga tgaagcgctt agcgagcgtc
     3301 tggaagagtg gatctgcggc gaaggtgcca gcgcgcagat cgacacgcag gggattcatt
     3361 tactcgcttg atgcgtcctg aataactggc gcaactacgg gttttgccgg gaagagatat
     3421 tttcccaacg tgaccagtac caccgcgaag acaattacgc cgagcgccag ccattcaatc
     3481 ttcgacagcg tctctccacc cagtcccgta cccagcaaga ccgcgaccac cgggttaacg
     3541 taagcgtagc tggtggcgag agccggactg acgttgcgga ttaaatacat ataagcgttg
     3601 atggcgataa tcgaaccaaa cagcgccaga tagccgaccg caaggaagcc tgaaagggaa
     3661 gggagcgccg tcagtttttc acctgcaatc atcgacgcga tcattaacac cacgccagcc
     3721 gccagcatct caatcgcacc cgccatcatc cctacaggta aggtaatgcg cgagccataa
     3781 actgagccaa acgcccagct aatcgagccg attaaaatca gaatcgcgcc ccacggattg
     3841 ccgcttaaat ttccaccgct attgagcatg atgattccgg caagcccaat ggcaataccc
     3901 acccattcca gtttgcgcgt tttaatgcca aacaggcggc tgaagcacag ggtaaagagg
     3961 ggcacggttg caaccactac ggcggcgatg ccggaaggaa cattttgatg ttcggcaacc
     4021 gtcaccatgc cattaccgac agccagcaat aacaggccaa tcagcgcggc attgaacagc
     4081 ggacgtagcg gggggagctt gtgtccgcgc agtagcaaaa atgccagcaa taaaataccg
     4141 gctgccagga atcgaacgcc cgccatcatt aacggaggcc agctttccac gccaatccga
     4201 atgacaaaat aggttgagcc ccaaatgata tacaacgcaa acagcgcgcc aaaaagcggt
     4261 aacaactggc ggaaacgcat aatccctcac ggtggaaata aaaaggtggt tcatagtaaa
     4321 cgtgaaaatc attctgctgg cgagagatat aattgcactt gattgttaaa aaaatgttga
     4381 cctgtgaagc aagtatcaga gctgccgttt ttgcttcata cttacaccct tcaacaataa
     4441 aaatgagagg gaatgctttt ggccgggagt agtttactga cgttgctcga tgatatcgcc
     4501 acactgctgg acgatatctc cgtgatgggc aaactggcgg cgaagaaaac cgccggtgta
     4561 ttaggggatg acttatcgct caatgcgcaa caagtttcag gcgtgcgggc caaccgggaa
     4621 cttcccgtgg tctggggcgt ggcgaaagga tcgctgatca ataaagtgat tctggtgcca
     4681 ctggcgctga ttatcagtgc gtttatcccg tgggcgatta cgcctctgtt gatgattggt
     4741 ggcgcgtttc tctgctttga aggggtagag aaagtgctgc atatgctgga ggcgcgtaaa
     4801 catagagaag atccggcgca gagccagcag cgtctggaga agctggcggc gcaggatccg
     4861 ctgaagtttg aaaaggacaa aataaaaggg gcgattcgta ccgattttat attgtctgcg
     4921 gaaatcgtcg ccatcacgct ggggattgtg gccgaagcgc cgttgcttaa tcaggtgctg
     4981 gtgctttcag gcatagcgct ggtagtgacc gtgggcgtct acggtctggt aggggttatc
     5041 gttaagattg atgacctggg gtattggctg gcggaaaaat ccagcgcgct gatgcaggca
     5101 ttaggtaaag gattattgat tatcgcgccc tggctgatga aagcgttatc gattgtcggc
     5161 acgctggcga tgttcctcgt cggcggcggg attgtggtac atggtattgc gccgctgcat
     5221 cacgccattg aacatttcgc cgggcagcaa agtgcagtgg tggcgatgat attaccgact
     5281 gttttaaatc tgattcttgg atttatcatc ggcggcatcg tggtgctggg agtgaaagcc
     5341 gtagcgaaaa tgcgcggtca ggcacattaa tactcgcaca ggctattatg caaaattcgc
     5401 catcttcaac taaggtgaac aagtttcact cgaaaaaagg aggcgagtat gagctttatg
     5461 gttagtgagg aagttacggt taaagagggc ggcccgcgga tgattgtcac tggatactcc
     5521 agcggtatgg ttgagtgtcg ttggtatgac ggttacgggg tcaagcggga agcttttcat
     5581 gaaaccgagc ttgttccggg ggaggggagt cgtcctgcgg aagaagtttg aaagggtatc
     5641 attaagacca ggccggatca catccggcct ggtgtgccaa tgcctgcaat gaatgatagt
     5701 cgcgtcttat catgccacca ttataaaatt atcgctgttt tgcggtacgc tatcgccagg
     5761 ttatgccaga atcataaaaa agcaggtggg gagtcgtcag ggtgcagcac gagacaaaaa
     5821 tggaaaacca gagctggttg aaaaaactcg cacgccgcct ggggcctggt catatcgtta
     5881 atctctgctt tatcgtggta ttgctttttt ccaccttgct gacctggcgt gaagtggtag
     5941 tgctggaaga tgcctatatc tccagccagc gtaatcatct ggaaaacgta gccaacgcgc
     6001 tcgataagca tttgcagtat aacgtcgaca aactgatctt tttgcgtaat ggcatgcgcg
     6061 aagctctcgt agcgccactg gatttcacct ctctgcgtga tgctgtaacc gagttcgaac
     6121 agcatcgcga cgagcacgcc tggaaaatcg aactcaaccg acggcgcacc ctgccagtca
     6181 atggcgtgtc ggatgcatta gtcagcgagg ggaatctcct gtctcgcgaa aatgaaagcc
     6241 tcgacaatga aattaccgct gcactggaag ttggttactt gctgcgacta gcgcacaact
     6301 cctcgtcgat ggttgaacag gcgatgtatg tctcgcgtgc cggattttac gtttcgacgc
     6361 agccgacctt gtttacgcgc aatgtcccga cgcgttatta cggctatgtc acccaaccct
     6421 ggtttatcgg ccattcacaa cgagaaaatc gtcaccgcgc ggtacgctgg ttcacttcgc
     6481 aaccggaaca cgccagcaat actgaaccgc aggttaccgt cagtgttccg gtagacagta
     6541 ataactactg gtatggcgtg ctggggatga gtattcccgt gcgtactatg cagcaatttt
     6601 taagaaacgc catcgataaa aacctcgatg gtgagtatca gctctatgac agtaagctga
     6661 gatttttgac ctcttccaat cctgaccatc caacagggaa tatttttgat cctcgtgaac
     6721 tggccatgct ggcgcaggca atggaacatg acacgcgggg cggcattcgt atggacagtc
     6781 gctatgttag ctgggaacgt ctggaccatt tcgacggtgt gctggtgcgc gtccatacgt
     6841 taagcgaagg cgtgcgcggc gatttcggca gtatcagcat tgcattaacc ctgctgtggg
     6901 cgctctttac caccatgtta ctcatctcct ggtatgtgat tcgccggatg gtcagcaaca
     6961 tgtatgttct gcaaagctcg ttgcagtggc aggcgtggca cgacacctta acgcgtttat
     7021 ataaccgtgg cgcactgttc gaaaaagccc gtccgctcgc gaaattgtgt cagacgcacc
     7081 aacatccttt ttctgtcatt caggtcgatc ttgaccattt taaagcgatt aatgaccgct
     7141 ttggtcatca ggcgggcgac cgtgttcttt ctcatgctgc cggattaatt agcagttcct
     7201 tgcgtgcgca ggacgttgcc gggcgggtcg gtggtgagga gttttgtgtg attctgccag
     7261 gcgcgagtct gacggaggct gcggaagtcg cagaacgtat tcgcctgaag ttaaatgaaa
     7321 aagagatgtt gattgctaag agtacgacga tacgcatcag tgcctcgttg ggggtaagta
     7381 gcagcgagga aaccggtgat tatgattttg aacaactcca gtcactggcc gaccgtcggc
     7441 tttatctcgc taaacaggcc gggcgtaatc gggtattcgc gagcgataac gcttaacggg
     7501 cggagaaaaa atggtccagc ccttcacgcc atccttccgg tccttcacgc tgcgttcgcc
     7561 agacgcgggc cggatcctca tcatgcagat gcaccccttc acggtttagc cctttcacaa
     7621 tcaccgcgta atccattacc tccagtaagg gcgcatcgtt tggcccatcg cccaggccaa
     7681 gtgtggttgg gcgtttgcct gacaattgtt gataggtcgc gataatccag ttggcagcct
     7741 gatcttttcc ggcagaggca tccaggacgt gccagaagcg cgcaccttgc ataaactgta
     7801 agcccagttc gttcagacga gcggtaaatt gtgccatacg ctcgtcactg tcgcgccaga
     7861 ttagcgttac cgacgcctca tgaagctgcg tcagcgccgc ctggctacgg cttaatcccg
     7921 tccattcggc gatggttgca tcgtcgacat catcaaaagt cgtgaattta aaatgttctt
     7981 tctcgcgtag cgtatttaaa acctggctga tttcgccatg gctaatacct gagatgatgc
     8041 gtggaaaacc gtctatctcc tgccattgct cagcaagctg gatcactgcg ccattttcgg
     8101 caatcagcgg taaaccttgt agccccaacg ttttttgcaa gtacagcatt tccgctgatg
     8161 ttttactgct acagagaatg acgggaacat ttgcttcgcg taaacgggtg agccaggggg
     8221 ctgccggttg ccagtcataa ctatgactgt ccagcagggt gccatcaaga tcgctaaaaa
     8281 ccagtagtgg ttgttgaatt gaaaacatag tcgcgcagta ctccccttac caggatgatt
     8341 ctcaaaacag cacaaattat caaagatgat ttttttcggg agcgatcccg ctgacgccag
     8401 agccaccaag gggtgacgtg cgtcacattt ctattcataa gtagcgttaa tcattcatat
     8461 ggcgaatatt ttcttgtcag cgaaaaaaat tgcggataag gtgatgaaca catcagattt
     8521 cctggtgtaa cgaatttttt aagtgcttct tgcttaagca agtttcatcc cgaccccctc
     8581 agggtcggga tttttttatt gtgcattcaa cgattcactt cagagacgct gaagtcatga
     8641 atatccagtt cgaaggcatc cgccaattca cgccatgtat gatattcacg tccatcgacg
     8701 ctaacgtgtt cagaatcgtt ctggctgcga tgaacttcgc tttcgctaat ttcattgatc
     8761 gccgccagca gggcatcgac atcgacgctg acctcacgtt ttgcggtatc gctgtactct
     8821 tttgcggttt tcatcatctt tcctcgcaac cgttactctg ttgttgccgc cagtcgttaa
     8881 gtccgcctgg catctcttaa gtatagaccg tcgagaaaat cagcactcgc gcgcgcctgg
     8941 cgctgccaga aaaacgaaat tgttctacac tggcacaaag ccacaggagg aaaacgatga
     9001 aggtgaatga tcgggtaaca gtcaaaacgg atggcggtcc gcgtcgtcct ggcgtggtac
     9061 tggcagttga ggagtttagt gaaggcacaa tgtacctggt ttcgctggaa gactacccgc
     9121 tcggcatctg gttctttaat gaagcagggc atcaggacgg tatctttgtg gagaaagcag
     9181 agtaatctaa ttcagcctga ctggtgggaa accaccagtc agaatgtgtt agcgcatgtt
     9241 gacaaaaata ccattagtca cattatccgt cagtcggacg acatggtaga taacctgttt
     9301 attatgcgtt ttgatcttac gtttaatatt acctttatgc gatgaaacgg tcttggcttt
     9361 gatattcatt tggtcagaga tttgaatggt tccctgacct gccatccaca ttcgcaacat
     9421 actcgattcg gttcggctca atgataacgt cggcatattt aaaaaagagg ttatcgttgt
     9481 ctcttttttc agaatatcgc caaggatatc gtcgagagat tccggtttaa tcgatttaga
     9541 actgatcaat aaattttttc tgaccaatag atattcatca aaatgaacat tggcaattgc
     9601 cataaaaacg ataaataacg tattgggatg ttgattaatg atgtgcttga tgcgctgact
     9661 gttagaagca tcatggatga aacagtcctc attaataaac accactgaag ggcgctgtga
     9721 atcacaagct atggcaaggt catcaacggt ttcaatgtcg ttgatttctc tttttttaac
     9781 ccctctactc aacagatacc cggttaaacc tagtcgggtg taactacata aatccataat
     9841 aatcgttgac atggcatacc ctcactcaat gcgtaacgat aattcccctt acctgaatat
     9901 ttcatcatga ctaaacggaa caacatgggt cacctaatgc gccactctcg cgatttttca
     9961 ggcggactta ctatcccgta aagtgttgta taatttgcct ggaattgtct taaagtaaag
    10021 taaatgttgc gatatgtgag tgagcttaaa acaaatattt cgccgcagga gtatcctgga
    10081 agatgttcg
//
LOCUS       DAZUCE010000187        10072 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZUCE010000187 DAZUCE010000000
VERSION     DAZUCE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.187"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            170..385
                     /gene="bdm"
                     /locus_tag="ACHY03_003898"
     CDS             170..385
                     /gene="bdm"
                     /locus_tag="ACHY03_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFS3907076.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            487..624
                     /gene="sra"
                     /locus_tag="ACHY03_003899"
     CDS             487..624
                     /gene="sra"
                     /locus_tag="ACHY03_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFS3907077.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            780..2477
                     /gene="maeA"
                     /locus_tag="ACHY03_003900"
     CDS             780..2477
                     /gene="maeA"
                     /locus_tag="ACHY03_003900"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFS3907078.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIEDNFWQAEYRDYRRTSI"
     gene            2611..3621
                     /gene="adhP"
                     /locus_tag="ACHY03_003901"
     CDS             2611..3621
                     /gene="adhP"
                     /locus_tag="ACHY03_003901"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFS3907079.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3767..4051
                     /locus_tag="ACHY03_003902"
     CDS             3767..4051
                     /locus_tag="ACHY03_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFS3907080.1"
                     /translation="MKMANHPRPGDIIQESLYELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4280..4933)
                     /gene="fdnI"
                     /locus_tag="ACHY03_003903"
     CDS             complement(4280..4933)
                     /gene="fdnI"
                     /locus_tag="ACHY03_003903"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFS3907081.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4926..5810)
                     /gene="fdnH"
                     /locus_tag="ACHY03_003904"
     CDS             complement(4926..5810)
                     /gene="fdnH"
                     /locus_tag="ACHY03_003904"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFS3907082.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5823..8870)
                     /gene="fdnG"
                     /locus_tag="ACHY03_003905"
     CDS             complement(5823..8870)
                     /gene="fdnG"
                     /locus_tag="ACHY03_003905"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8283..8285),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFS3907083.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9102..9983
                     /gene="yddG"
                     /locus_tag="ACHY03_003906"
     CDS             9102..9983
                     /gene="yddG"
                     /locus_tag="ACHY03_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFS3907084.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSRPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2467 a         2641 c         2532 g         2432 t
ORIGIN      
        1 caactcacca caataataat acctatcaat atcatggggt taaaatataa aaatcatcaa
       61 ccaggactaa tcttaacaac gaagcggcaa atatttgcca tgctgaatgt gctttataaa
      121 tctgcgatcc gtagcagaca ccataaatac acagacacgg agaatcacta tgtttactta
      181 ttatcaggca gaaaattcaa cagcagaacc cgctctggtt aatgccattg agcaaggcct
      241 tcgggcagag cacggtgttg tcactgaaga tgacattctc atggagctga ccaaatgggt
      301 ggaagcttct gataacgaca tcctcagtga tatctaccag caaacgatca attatgtggt
      361 cagtggccag caccctacgc tttaaggtgc tatgcttgat cggcaaccta atttaggggt
      421 ttagcacgtg tttcttcgct acggcgatgt tgtccttaaa actagcaaca ggattgagga
      481 gttaaaatga aatcgaaccg tcaggcacgt catattcttg gactggacca taaaatatct
      541 aaccagcgca aaatagttac cgaaggtgac aaatctagcg tggtaaataa cccaaccggc
      601 agaaaacgcc ccgctgaaaa gtaattcata accatcagtc ctcaatgacg attaaacacc
      661 attgcctgcg caatggtgtt tttgttttta tctgctttat acttggggcc gacgccctgg
      721 cggtaaagca aagacgataa aagcccccag ggatggatat tcaaaaaaga gtgagtgaca
      781 tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc ttacgctggc cctgtactgc
      841 tggaatttcc gttgttgaat aaaggcagtg ccttcagcat ggaagaacgc cgtaacttca
      901 acctgctggg gttactgccg gaagtggtcg aaaccatcga agaacaagcg gaacgagcat
      961 ggatccagta tcagggattc aaaaccgaaa tcgacaaaca catctacctg cgtaacatcc
     1021 aggacaccaa cgaaaccctc ttctaccgtc tggtaaacaa tcatcttgat gagatgatgc
     1081 ctgttattta taccccaacc gtcggcgcag cctgtgagcg tttttctgag atctaccgcc
     1141 gttcacgcgg cgtgtttatc tcttaccaga accgccacaa tatggacgat attctgcaaa
     1201 acgtgccgaa ccataatatt aaagtgattg tggtgactga cggtgaacgt attctggggc
     1261 ttggtgacca gggcatcggc gggatgggca ttccgatcgg taaactgtcg ctctataccg
     1321 cctgtggcgg tatcagcccg gcgtataccc ttccggtggt gctggatgtc ggcaccaaca
     1381 accaacagct gcttaacgat ccgctgtata tgggctggcg taatccgcgt atcactgacg
     1441 acgaatacta tgaattcgtt gatgaattta tccaggctgt gaaacaacgc tggccggacg
     1501 tgctgttgca gtttgaagac ttcgcacaaa aaaatgcgat gccgttactt aaccgctatc
     1561 gcaatgaaat ttgttctttt aacgatgaca ttcagggcac tgcggcggta acagtcggca
     1621 cactgatcgc agcaagccgc gcggcaggtg gtcagttaag cgagaaaaaa atcgtcttcc
     1681 ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat catcgcccag acccagcgcg
     1741 aaggattaag cgaggaagca gcacggcaga aagtctttat ggtcgatcgc tttggcctgc
     1801 tgaccgacaa gatgccgaac ctgctgcctt tccagaccaa actggtgcag aagcgcgaaa
     1861 acctcagtga ctgggatacc gacagcgatg tgttgtcact gctggatgtg gtgcgcaatg
     1921 taaaaccaga tattctgatt ggcgtctcag gacagaccgg gctgtttacg gaagagatca
     1981 tccgtgagat gcataaacac tgtccgcgtc cgatcgtgat gccgctgtct aacccgacgt
     2041 cacgcgtgga agccacaccg caggacatta tcgcctggac tgaaggtaac gcgctggtcg
     2101 ccacgggcag tccgtttaat ccagtggtat ggaaagataa aatctaccct atcgcccagt
     2161 gtaacaacgc ctttattttc ccaggcatcg ggctgggtgt tattgcttcc ggcgcgtcac
     2221 gtatcaccga tgagatgctg atgtcggcaa gtgaaacgct tgctcagtat tcgccgctgg
     2281 tcctgaacgg cgaaggtctg gtactgccgg aactgaaaga tattcagaaa gtctcccgcg
     2341 caattgcgtt tgcggttggc aaaatggcgc agcagcaagg cgtggcggtg aaaacctctg
     2401 ccgaagccct gcaacaggcc attgaagata atttctggca agccgaatat cgcgactacc
     2461 gccgtacctc catctaagcc tgcgcccggt agtgaaggct accgggctat ttccctctcc
     2521 ctttttcaga tctcatccat actgggtagt ggcgaataaa tctcatttgc ctcacctgct
     2581 atgcagaaca tcatccgaaa aggaggaact atgaaggctg cagttgttac gaaggatcat
     2641 catgttgacg ttacggataa aacactgcgc tcactgaaac atggcgaagc cctgctgaaa
     2701 atggagtgtt gtggtgtatg tcataccgat cttcatgtta agaatggcga ttttggtgac
     2761 aaaaccggcg taattctggg ccatgaaggt atcggtgtgg tggcagaagt gggtccaggt
     2821 gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt ggttttacga aggatgcggt
     2881 cattgcgaat actgtaacag tggtaacgaa acgctctgcc gttcagttaa aaatgccgga
     2941 tacagcgttg atggcgggat ggcggaagag tgcatcgtgg tcgccgatta cgcggtaaaa
     3001 gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg ggtcaccacc
     3061 tacaaagccg ttaagctgtc aaaaattcgt cccgggcagt ggattgctat ctacggtctt
     3121 ggcggtctgg gtaacctcgc cctgcaatac gcgaagaatg tctttaacgc caaagtgatc
     3181 gccattgatg tcaatgatga gcagttaaaa ctggcaaccg aaatgggtgc agatttagcg
     3241 attaactcac gcaccgaaga cgccgccaaa attgtgcagg agaaaaccgg tggcgctcac
     3301 gctgcggtgg taacagcagt agctaaagct gcgtttaact cggcagttga tgctgtccgt
     3361 gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt ctatgagcct ggatatccca
     3421 cgtcttgtgc tggatggcat tgaagtggtc ggttcgctgg tcggcacgcg ccaggatcta
     3481 actgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc cctgcgtccg
     3541 ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg tggccgtatg
     3601 gtgattgatt tccgtcgcta agaggccttt gctgcgactg ccatgttcgg gtcgcagcat
     3661 cgcacactct ccaacatgaa atggctaaat ggattattgt tgcgtaggaa ggaacaataa
     3721 tgatagtgtt cccagagaag gaacgctact gagagtaaag acactaatga aaatggccaa
     3781 tcatccccgc ccgggggaca ttattcagga atcactgtac gaacttaatg tcagcctgcg
     3841 cgagtttgcc agagcaatgg aaattgctcc ctcaacggca agccgattgc tgacaggaaa
     3901 agcagctttg acgccagaaa tggcaattaa actttccgtg gtgatcggca gttcgccgca
     3961 aatgtggctg aatctgcaaa acgcctggag tctggcagag gcagaaaaaa cggtggatgt
     4021 gtcgcgatta cgccgtctgg ttacgcaata aaaaaatggc accttataaa gcgccatttg
     4081 aaactgataa caaacgcaac attgcccgat gcgctacgct tatcaggcct acacgttcac
     4141 tgcaatctat tggatttgaa cggtttgtag gccggataag gcgttcacgc cgcatccggc
     4201 atgaacaaag cctacgttgt ctacaatatg aaaatggcgc cttttacagc gccattttca
     4261 gcttaaagtt caaaagagat tatatccctt cttcactctc ttttttcgct tctgccttct
     4321 cgatttcacg ataccagcgc ggatggtgtt tcttcgccca gcgacggctc accttccctt
     4381 cgatcatccc tttaatcgat cctttcaccc agaatgccat atacatatgg atcaggatgg
     4441 cgtggatcag gatgataccc gcagccgcgt ggatcagcag gctataacgg acaacctgca
     4501 tcgggaagta ctgcgcaaag tacggacgcc agataatcac cccggtcacc aacagcacga
     4561 aaatcatgct catgatcgac cagaacatca tcttttgccc ggcgttgtac ttaccaacat
     4621 ccgccacttt atgctcattg cctttcaata cttcgacaat gttcaacagc cacggaatat
     4681 ctttcttatc cgggatgttg tgatgcacaa aacgcacaaa cataaacatc agtgcgacga
     4741 aaatcgcaat gccgaagaac gggtgcaaaa tgcgtcccat ctgcggcgtg ccgaaggttt
     4801 gcgtcagcca ttgcagcgtc gggaagaaga acgaaatccc ggacagcgcc accaggaaga
     4861 agcaaatcac cacggtccag tgacaggcgc gatcaataaa tttggtgcgc acaatcattt
     4921 tcgacttact catgatgatc ctcctcgtca tcgtccactt ccttattcgg gccaataccg
     4981 atgtagtgga aaatcaaccc ggcaaaagtg gcaataaagc cagccgctgc cagcggtttc
     5041 aacgcgcctt tccacaggct taccgaggtg tcgatcttcg gatctttcgg cagaccgtga
     5101 tacagctccg gctgatcggc gtgatgcagc acgtacataa cgtgcgtacc accgacccct
     5161 tccgggttgt agacgccagc atgttcgtaa ccacgcgctt tcagtttcgc cacgcgctgt
     5221 tccgccagct ccagcatctc cttcttggtg ccgaagtgga tagccccggt cggacaggtt
     5281 ttcacacaag ccggttcctg gccgacgctg acgcgatcga cgcagagcgt gcatttatat
     5341 acccggttat cctctttgtt gaggcgcgga atattaaacg gacacccggc aatgcagtaa
     5401 ccacagccga tgcagttttc cgactggaaa tcgacaatcc cgttagcgta ctgaatgatt
     5461 gcaccagcag acgggcacgc cttcaggcag cccggatctt cacagtgcat acagccgtct
     5521 ttacggatca gccactccag cttgccgttc tgttcggttt cgctaaagcg catcaccgtc
     5581 caggacttgg cgctcagatc ggcggggtta tcgtaaaccc cgacgcagtg ccccacttca
     5641 tcacggatgt cgttccactc cgaacacgcc acctgacagg ctttacaacc gatacaggtg
     5701 gaaacgtcga taagtttcgc gacttctgct ttgtaatcac gcacctgaga aggcggcgtg
     5761 atggagttag ttgcggacct tttgataatg tcctgcgttt ccatagccat ctgttcgccc
     5821 ccttacgcct tctcgatgtt gactaagaac gctttatatt ccggcgtttg cgagtttgca
     5881 tcaccgacat tcggcgtcag agtgttagcg atataacctt tacgcgcgac accctcaaag
     5941 ccccagtgga ttggaatacc caccgtttca acctgctgac cgtttacatt cagcggtttc
     6001 agacggcgcg tgaccacagc caccgcgcgg ataaagccac gcttgcttga gacagtgaca
     6061 cgatcgccat tattaatgcc tttcgccgcc gccagcgttt cgctgatttc cacaaactgt
     6121 tccggctgag caattgcgtt gagcaatgcg tgcttggtcc aggtgtggaa gtgctcggtc
     6181 agacgatagg tcgtacccac atacgggaac tgctcttttt tacccatccg cagtgcgtct
     6241 tgttcataca gacgaacaac cgggttagac accacgttcg gatgcagcgg gttagtgccc
     6301 agcggcgttt caatcggctc gtagtgttcc gggaacggac cttccgccat tttgttgatg
     6361 gcaaacaggc gtcccatccc ttccggctgc atgataaacg gcccggttgg cgtacccggt
     6421 gcggcattgc cgaagtccgg aatatcgtta cccgtccact tgctgccgtt ccactggatc
     6481 agcatccgtt tcggatccca cggtttaccg ttgatatccg ccgaagcacg gttgtacagc
     6541 acgcgacggt tgagcggcca cgcccaggcc catcccagcg tattccccag accggacggg
     6601 tcggagttat cgcggttagc catctggttg ccctgctctg tccagctacc ggtgtagatc
     6661 cagcaagaag atgcggttgt accgtcatca cgcagatgcg caaagctact cagcaactga
     6721 cctttcttcg caatcagcac gccattagca tcatagagat cttccagcgc atagccgttg
     6781 ttctcttttg ccacttcgtc agattgcggt tcgtgcggct gcttgtagtt ccagctcatc
     6841 ttcatcagcg gttctacgcc tttaccacct tcggcctggt acagctcgcg cagatgatgg
     6901 tagatacccg ccagaatttc gccgtcgtta cgcgcttcgc ccggcgcatc ctgacctttc
     6961 cagtgccact gcagccagcg accggagtta gcaatagaac catcttcttc agcaaagcag
     7021 gtcgaaggca gacggaatac ttcagtctga atagacgccg gatcgacatc gttcgactcg
     7081 ccgtggttct gccagaaggt tgaggtttca gtcaccagcg gatcgataac caccatgtac
     7141 ttcagcttgc tcaggcagct caccactttg tttttgtccg ggaaggacgc aaccgggtta
     7201 aagccctggc agaaataacc ggtgactttg ccttcatcca tcatgttgaa atacttgatg
     7261 acgtcgtagg tctggtccca cttcggcagc cagtcatagc cccagttgtt ctctttctgc
     7321 gcggcatcgc catagaaaga tttcatcagg ctaacgaaga acttcggata gttgctccag
     7381 tagttcacct gaccagccag cgtcgctttc ggcgtgttcg cttccagata cgactgcaaa
     7441 tcaacctgtt tttctgacgg cagcgtcaga taacctggca ggctggtaga gagcagacct
     7501 aagtcagtca gaccctgaat gttggagtga ccacgcaatg cattcacgcc gccaccggcc
     7561 atacccatgt taccgagcag caactggatc atcgccatag tacggatgtt ctgcgcaccc
     7621 acggtgtgct gcgtccagcc cagcgcgtac aggaaggtgg ttgtgcgatc cggtgcgctg
     7681 gtggaggcca gcacttcaca cactttcagg aagtcggctt ttggcgtacc acagatgttt
     7741 tcaacaacgt ccggcgtgta gcgggaaacg tgcgctttca gcaggttcca cacacagcgc
     7801 ggatgagtca gtgtttcatc gcgtttcgca tagccgtttt catcgaactg atagttccag
     7861 gacgatttat cgtattgccg tttttcagcg tcgtagccgc tgaacagacc gtcttcgaaa
     7921 gcaaaatcat cacgcaccag caggctggcg ttggtgtaat gcttaacgta ttcggcgttg
     7981 attttgttgt tttcgatcag gtagcgcaaa acgccagaca ggaacgtaat gtccgtaccg
     8041 gaacgaatag gtgcgtaaat atccgccaca gaagcggtac gcgtaaaacg gggatcgaca
     8101 acgatcaagg ttgcatcgtt gttgtttttc gcttccatcg cccagcggaa accgacggga
     8161 tgcgcttcag cagcgttacc gcccattacc attacgacgt tagcgttttt gatatccacc
     8221 cagtggttgg tcatcgcacc gcgaccaaat gttggagcaa gacttgctac cgttggtccg
     8281 tgtcagacgc gcgcctggtt gtctaccgcc agcatcccga gggagcgggc aaatttctgg
     8341 gtcagcatcc cggtttcgtt gctggcaccg gaagcacaca gcataccggt agaaagccaa
     8401 cggtttaccg ttacgccctg ctcgttcttt tcaataaagt tagcgtcacg gtcagctttc
     8461 atcagcttcg caatacggga gaatgcttct tcccagctaa tgcgctgcca tttgtcagaa
     8521 cctggcgcac gatattccgg gtagcgcaga cggttttcgc tgttgacgta atccagcaaa
     8581 ccggcccctt tggggcacag cgcaccacgg cttaccggat gatccgggtc accttcaatg
     8641 tgataaatcg cttctctggc gttttttgcg ccatcaccca ggctatacat caatagcccg
     8701 caacctacgg aacagtatgt gcaggtgtta cggatctctt tagcgcgtaa taatttgtag
     8761 tttcgcgcct gagccagtgc ttgcttcggg gcaaagccca atgccgctac tgttgttcca
     8821 gccataccgc ccgcgcagat tttaaaaaat tgtctgcgac tgacgtccat tgctttcctc
     8881 ttttttcagg ggtattactg cgcgaggaaa ctaacagtaa aattctgaat ctttttgcgc
     8941 gaaatcaaaa agaacacgtg tttgccacaa aataatccgc ctgcgcaaat tgacctacct
     9001 caatagcggt agaaaaacgc accactgcct gacaggccag ttaaaaaaat gctataaaat
     9061 tcagcttaat ttttaacggc aagagagaca aaacagcgag catgacacga caaaaagcaa
     9121 cgctcatagg actaatagcg atcgtcctgt ggagcacgat ggtaggattg attcgcggtg
     9181 tcagtgaggg gctcggcccg gtcggcggcg cagctgctat ctattcatta agcgggctgc
     9241 tgttaatctt cacggttgga tttccgcgaa ttcggcaaat cccgaaaggc tatttactcg
     9301 ccgggagtct gttattcgtc agctatgaaa tctgtctggc gctttcctta gggtatgcgg
     9361 cgacccgtca tcaggcgatt gaagtgggta tggtgaacta tctatggccc agcctgacaa
     9421 ttctctttgc cattctgttt aatggtcaga aaaccaactg gttgattgta cctggattat
     9481 tattagccct cgtcggcgtc tgctgggtgt taggcggtga caatgggtta cattatgatg
     9541 aaatcatcaa taatatcacc accagcccat tgagttattt cctggcgttc attggtgcgt
     9601 ttatctgggc agcctactgc acagtaacga ataaatacgc acgcggattt aatggaatta
     9661 ccgtttttgt cctgctaacg ggagcaagtc tgtgggttta ctattttctc acgccacaac
     9721 cagaaatggt atttagcagg ccagtcatga ttaaactcat ctctgcggca tttaccttag
     9781 gatttgctta tgctgcatgg aatgtcggta tactgcatgg caacgtcacc ataatggcgg
     9841 taggttcgta ttttacgccc gtactttcct cagcgcttgc agccgtgctg ctcagcgccc
     9901 cgctgtcgtt ctcgttctgg caaggcgcgc tgatggtctg cggcggttcc ctgctctgct
     9961 ggctggcgac acgtcgtggc taaaataatt gccgggtcta cggcccggca attaatcaga
    10021 taaactccga caatattaaa aaataaattt caaataccag taattcacct ta
//
LOCUS       DAZUCE010000188        10042 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZUCE010000188 DAZUCE010000000
VERSION     DAZUCE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.188"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..536)
                     /locus_tag="ACHY03_003907"
     CDS             complement(<1..536)
                     /locus_tag="ACHY03_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFS3907085.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVS"
     gene            complement(755..1732)
                     /gene="epmA"
                     /locus_tag="ACHY03_003908"
     CDS             complement(755..1732)
                     /gene="epmA"
                     /locus_tag="ACHY03_003908"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFS3907086.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2057..3865
                     /gene="frdA"
                     /locus_tag="ACHY03_003909"
     CDS             2057..3865
                     /gene="frdA"
                     /locus_tag="ACHY03_003909"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFS3907087.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3858..4592
                     /gene="frdB"
                     /locus_tag="ACHY03_003910"
     CDS             3858..4592
                     /gene="frdB"
                     /locus_tag="ACHY03_003910"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFS3907088.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4603..4998
                     /gene="frdC"
                     /locus_tag="ACHY03_003911"
     CDS             4603..4998
                     /gene="frdC"
                     /locus_tag="ACHY03_003911"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFS3907089.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5009..5368
                     /gene="frdD"
                     /locus_tag="ACHY03_003912"
     CDS             5009..5368
                     /gene="frdD"
                     /locus_tag="ACHY03_003912"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFS3907090.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5431..6564
                     /locus_tag="ACHY03_003913"
     CDS             5431..6564
                     /locus_tag="ACHY03_003913"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFS3907091.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELIAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6653..7186
                     /gene="blc"
                     /locus_tag="ACHY03_003914"
     CDS             6653..7186
                     /gene="blc"
                     /locus_tag="ACHY03_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFS3907092.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7183..7500)
                     /gene="sugE"
                     /locus_tag="ACHY03_003915"
     CDS             complement(7183..7500)
                     /gene="sugE"
                     /locus_tag="ACHY03_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFS3907093.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7681..7827)
                     /gene="ecnB"
                     /locus_tag="ACHY03_003916"
     CDS             complement(7681..7827)
                     /gene="ecnB"
                     /locus_tag="ACHY03_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFS3907094.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7938..8063)
                     /gene="ecnA"
                     /locus_tag="ACHY03_003917"
     CDS             complement(7938..8063)
                     /gene="ecnA"
                     /locus_tag="ACHY03_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFS3907095.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8115..8681)
                     /gene="efp"
                     /locus_tag="ACHY03_003918"
     CDS             complement(8115..8681)
                     /gene="efp"
                     /locus_tag="ACHY03_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS3907096.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8723..9751
                     /gene="epmB"
                     /locus_tag="ACHY03_003919"
     CDS             8723..9751
                     /gene="epmB"
                     /locus_tag="ACHY03_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFS3907097.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2576 a         2586 c         2538 g         2342 t
ORIGIN      
        1 atgcttacta acagcaatac taaattcaga cacattactg caataccgcc caccgcagta
       61 atgcgggcaa ttttattaat ccccttagaa gcaacgacgg tgaccagaat catccatgcc
      121 actgccagca gaccaaccac ctgcgtaggc tccagtccgg caatacgcca gtgctgggtc
      181 atgtcgctac catagaggaa tgttgagaac ggtacccaaa ctttcgcgga ggtactcacc
      241 atccagatga tataagagga aaaccacata aacgtaccaa tgaaggcaaa acgtggtccg
      301 acactattat tcatccagga atagatacca ccttcttctt tgcgataagc agctcccatt
      361 tcagccatca ttaaggcgaa tggaataaag aataacaatg cagaaaatat ataaaaggga
      421 atcgcactat aacccattaa gtaataagcc gatgggctat tggcaaatcc aaatacggaa
      481 gtaaagatca tcaatatgag tcctatcaga ctcatctttt ttatcgtgtg aggcatgaaa
      541 ccatccttca tttcagcgcg tgtataaaac gcgcccactg aactgcactg cccaacattg
      601 agcgggcagc gtaaaaacca atacagaatt gatacctgga tgatatcaaa tatccagtct
      661 taaattgtgg cctttagcgg aaaaatgaaa acatatgcta cagaatggcg cttatcacgc
      721 cattcttcgc tgttaattca gtaatttttc agaattatgc ccggtcaacg ctaaaggcga
      781 tgacttcagc cagtgtctcc gcacccagcg ccaacatcac cagacgatca acgcctaatg
      841 ccacgccgga acagtcaggc ataccgactt tcaaggcttc aatcagattc tggtcaatgg
      901 ggtgctgcgg caaaccgcgc gccgcgcgct tacggttatc ttgttcaaag cgttgttgct
      961 gctcacgggc atccgtcaat tcatggaaac cattcgccag ctcaatacct ttataataaa
     1021 cctcaaagcg ttcagcgacc cgatgatctt cggtactgat ttgcgccagt gatgcctggc
     1081 tggctggaaa gtggtacaca aaggtcggtt tttctttacc aatatttggc tctacgccaa
     1141 aggtaaacag caattgtagc aacgtgtcgc ggtcttcttc ggtatcagca acattgctca
     1201 aatccagttt cgctgccact tcccgcagtt gcgttttgtc ggcagagagt gggtcaattt
     1261 ccagataacg caagaaagct tgttgataag aaaggctttc tgctgccggg cagtccagca
     1321 cctgttgtaa gagatcgtcc acctcgttca tcaaccggta catatcatag tgcggtcgat
     1381 accactcaag catagtgaac tcagggttgt gataacgccc catctcttca ttacggaagc
     1441 tgcggcacag ctggaatacc ggcccacaac cagcaaccag caggcgtttc atatggtatt
     1501 cagggctggt cattaaccag agattcatcc cctgcgaatg cccggggcca acgaaacgtg
     1561 tctcaaacgg gaccaaatga atatcggtta ccgtcgcctg gctcatacaa ggcgtctcca
     1621 cctccagcac tccacgatcg gcaaagaaac gacggatctc cgccataatc gccgcgcgtt
     1681 ttaataagtt aggaatggat gcgctcggct gccaggatgc cgtttcgctc atagttaaat
     1741 ctccagtttt tgacaagggc acgaagtcta ctcgcaacgc gacggcgaga caaattttac
     1801 gcaggaatca aacaacggtg ggcagtgact aaaaaaagca cgatctgatg gtttagtaat
     1861 taaattaatc atcttcagtg ataatttagc cctcttgcgc actaaaaaaa tcgatctcgt
     1921 caaatttcag acttatccat cagactatac tgttgtacct ataaaggagc agtggaatag
     1981 cgttcgcaga ccgtaacttt caggcactta ccctgaagta cggggctgtg ggataaaaac
     2041 aatctggagg aatgtcgtgc aaacctttca agccgatctt gccattgtag gcgccggtgg
     2101 cgcgggatta cgtgctgcaa ttgctgccgc gcaggcaaat ccaaatgcaa aaatcgcact
     2161 aatctcaaaa gtatacccga tgcgtagcca taccgttgct gcagaagggg gctccgccgc
     2221 tgtcgcgcag gatcatgaca gcttcgaata tcactttcac gatacagtag cgggtggcga
     2281 ctggttgtgt gagcaggatg tcgtggatta tttcgtccac cactgcccaa ccgaaatgac
     2341 ccaactggaa ctgtgggggt gcccatggag ccgtcgcccg gatggtagcg tcaacgtacg
     2401 tcgcttcggc ggcatgaaaa tcgagcgtac ctggttcgcc gccgataaga ccggcttcca
     2461 tatgctgcac acgctgttcc agacctctct gcaattcccg cagatccagc gttttgacga
     2521 acatttcgtg ctggatattc tggttgatga tggtcatgtt cgcggcctgg tagcaatgaa
     2581 catgatggaa ggcacgctgg tgcagatccg tgctaacgcg gtcgttatgg ctactggcgg
     2641 tgcgggtcgc gtttatcgtt acaacaccaa cggtggcatc gttaccggtg acggtatggg
     2701 tatggcgcta agccacggcg ttccgctgcg tgacatggaa ttcgttcagt atcacccaac
     2761 cggtctgcca ggttccggta tcctgatgac cgaaggttgc cgtggcgaag gcggtattct
     2821 ggtcaacaaa aatggctacc gttatctgca agattacggc atgggcccgg aaactccgct
     2881 gggcgagccg aaaaacaaat atatggaact gggtccacgc gacaaagttt ctcaggcctt
     2941 ctggcacgaa tggcgtaaag gcaacaccat ctccacgccg cgtggcgatg tggtttatct
     3001 cgacttgcgt cacctcggcg agaaaaaact gcatgaacgt ctgccgttca tctgcgaact
     3061 ggcgaaagcg tacgttggcg tcgatccggt taaagaacca attccggtac gtccgaccgc
     3121 acactacacc atgggcggta tcgaaaccga tcagaactgt gaaacccgca ttaaaggtct
     3181 gttcgccgtg ggtgaatgtt cctctgttgg tctgcacggt gcaaaccgtc tgggttctaa
     3241 ctccctggcg gaactggtgg tcttcggccg tctggccggt gaacaagcga cagagcgtgc
     3301 agcaactgcc ggtaatggca acgaagcggc aattgaagca caggcagctg gcgttgaaca
     3361 acgtctgaaa gatctggtta accaggatgg cggcgaaaac tgggcgaaga tccgcgacga
     3421 aatgggcctg gctatggaag aaggctgcgg tatctaccgt acgccggaac tgatgcagaa
     3481 aaccatcgac aagctggcag agctgcagga acgcttcaag cgcgtgcgca tcaccgacac
     3541 ttccagcgtg ttcaacaccg acctgctcta caccattgaa ctgggccacg gtctgaacgt
     3601 tgctgaatgt atggcgcact ccgcaatggc acgtaaagag tcccgcggcg cacaccagcg
     3661 tctggacgaa ggttgcaccg agcgtgacga cgtcaacttc ctcaaacaca ccctcgcctt
     3721 ccgcgatgct gatggcacga ctcgcctgga gtacagcgac gtgaagatta ctacgctgcc
     3781 gccagctaaa cgcgtttacg gtggcgaagc ggatgcagcc gataaggcgg aagcagccaa
     3841 taagaaggag aaggcgaatg gctgagatga aaaacctgaa aattgaggtg gtgcgctata
     3901 acccggaagt cgataccgca ccgcatagcg cattctatga agtgccttat gacgcaacta
     3961 cctcattact ggatgcgctg ggctacatca aagacaacct ggcaccggac ctgagctacc
     4021 gctggtcctg ccgtatggcg atttgtggtt cctgcggcat gatggttaac aacgtgccaa
     4081 aactggcatg taaaaccttc ctgcgtgatt acaccgacgg tatgaaggtt gaagcgctag
     4141 ctaacttccc gattgaacgc gatctggtgg tcgatatgac ccacttcatc gaaagtctgg
     4201 aagcgatcaa accgtacatc atcggcaact cccgcaccgc ggatcagggt actaacatcc
     4261 agaccccggc gcagatggcc aagtatcacc agttctccgg ttgcatcaac tgtggtctgt
     4321 gctacgccgc gtgcccgcag tttggcctga acccagagtt catcggtccg gctgccatta
     4381 cgctggcgca tcgttataac gaagatagcc gcgaccacgg taagaaggag cgtatggcgc
     4441 agttgaacag ccagaacggc gtatggagct gtactttcgt gggctactgc tccgaagtct
     4501 gcccgaaaca cgtcgatccg gctgcggcca ttcagcaggg caaagtagaa agttcgaaag
     4561 actttcttat cgcgaccctg aaaccacgct aaggagtgca acatgacgac taaacgtaaa
     4621 ccgtatgtac ggccaatgac gtccacctgg tggaaaaaat tgccgtttta tcgcttttac
     4681 atgctgcgcg aaggcacggc ggttccggct gtgtggttca gcattgaact gattttcggg
     4741 ctgtttgccc tgaaaaatgg cccggaagcc tgggcgggat tcgtcgactt tttacaaaac
     4801 ccggttattg tgatcattaa cctgatcact ctggcggcag ctctgctgca caccaaaacc
     4861 tggtttgaac tggcaccgaa agcggccaat atcattgtaa aagacgaaaa aatgggacca
     4921 gagccaatta tcaaaagtct ctgggcggta actgtggttg ccaccatcgt aatcctgttt
     4981 gttgccctgt actggtaagg agcctgagat gattaatcca aatccaaagc gttctgacga
     5041 accggtattc tggggcctct tcggggccgg tggtatgtgg agcgcaatta ttgcgccggt
     5101 gatgatcctg ctggtgggta ttctgctgcc gctggggctg tttccgggtg atgcactgag
     5161 ctacgagcgc gttctggcgt tcgcgcagag cttcattggt cgcgtattcc tgttcctgat
     5221 gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc acgatctgaa
     5281 aatccacgta cctgcgggca aatgggtttt ctacggtctg gctgctatcc tgacagttgt
     5341 cacgctgatt ggtgtcgtta caatctaacg catcgccaat gtaaacccgg cccgcctatg
     5401 gcgggccgtt ttgtatggaa accagaccct atgttcaaaa cgacgctctg cgccttatta
     5461 attaccgcct cttgctccac atttgctgcc cctcaacaaa tcaacgatat tgtgcatcgc
     5521 acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc ggtaatttat
     5581 cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa gcagcccgtc
     5641 acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg cgtgcttggt
     5701 ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccgcaacaaa atactggcct
     5761 gaacttatcg ctaaacagtg gaatgggatc acactattac atctcgcaac ctacactgct
     5821 ggcggcctgc cattgcaagt gccggatgaa gtgaaatcct caagcgactt gctgcgcttc
     5881 tatcaaaact ggcagcctgc atgggctcca ggaacacaac gtctgtatgc caactccagt
     5941 atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga gcaggcgatg
     6001 caaactcgtg tcttccagcc actaaaactc aaccatacgt ggattaatgt accgtccgca
     6061 gaagaaaaga attacgcctg gggatatcgc gaaggcaagg cagttcatgt ttcgccaggg
     6121 gcgttagatg ctgaaactta tggtgtgaag tcgaccattg aagatatggc ctgctgggta
     6181 cgaagcaata tgaatccccg tgatatcaac gacaaaacac ttcagcaagg gatacaactg
     6241 gcacaatctc gctactggca aaccggcgat atgtatcagg gtctgggctg ggaaatgctg
     6301 gactggccgg taaatcctga catcatcgtt aacggcagtg acaataaaat tgcactggca
     6361 gcacgccccg taaaagcgat tacgcctcca actcctgcag tacgcgcatc atgggtgcat
     6421 aaaacggggg cgaccggcgg atttggtagc tatgtcgcgt ttattccaga aaaagagctg
     6481 ggtatcgtga tgctggctaa caaaaactat cccaatccag cgagagtcgc cgccgcctgg
     6541 cagattctca acgctctgca gtaaaatttc atcgggtccg aattttcgga ccttttctcc
     6601 gcttttcctt gctgtcatct acacttagaa aaaaaccagt aaggaaacat ttatgcgcct
     6661 gctccctctc gttgccgcag cgacagctgc atttctggtc gttgcctgca gttctcctac
     6721 gcccccgcgt ggcgtgaccg tagtgaataa tttcgacgct aaacgctatc tcggtacctg
     6781 gtatgagatt gcccgttttg atcaccgctt tgaacgtgga ctggaaaaag tcaccgcaac
     6841 atacagcctg cgtgatgacg gcggcctgaa tgtcattaat aaaggctata accctgacag
     6901 aggaatgtgg cagcagagtg aagggaaagc gtactttacc ggcgcaccaa ctcgcgctgc
     6961 gctgaaagtg tcattctttg gtcctttcta tggcggttat aacgttattg cactcgatcg
     7021 ggaataccgc catgcgctgg tttgcggccc ggaccgcgac tacctgtgga tactctcccg
     7081 cacgccaacc atttctgacg aagtgaaaca ggagatgctg gcagtcgcga cccgggaagg
     7141 gtttgatgtc agtaagttta tttgggtaca gcagcccggt agttagtgag tgctgagttt
     7201 cagaccaata atccccaata cgattaacgc cagactcgcc agacgcatcg ggttagcgga
     7261 ctcaccgagc agcacaatgc cggtgatggc cgcgccgact gcgccaatac ccgtccacac
     7321 ggcataagcc gtccctactg gtaacgattt catcgcccag gcaagtagcg ccatactgac
     7381 aatcatcgcc gtcacggtaa taacactcgg cgtcaaacga ctaaagccgt gggtatattt
     7441 caggccaacg gcccatacca cttccagcag accagcaata actaagataa tccaggacat
     7501 atcaggctcc ggagaattgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg
     7561 caaagctgat ggttgaacgg atattttgcc aggtggcaga ggaatttcaa ctattacctt
     7621 tgtgactatc gcaaaaaaac aaacggcacg acacagctgt cgtaccgtta ttgcttattg
     7681 ctattgctgc gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc
     7741 accaacgcca cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa
     7801 gatcgctgca attgtcttct tcaccataac gtcttccttt taggtaatgt tatttatgtt
     7861 tgcctatagc aaattaataa tagacaacat cgccaaaaat gacggaaatc tgatgatttt
     7921 tcggaagaga agaaaaatta gctggcagcg cgggagatgg agttgccgag atgtttgatg
     7981 tcttcgccga aaccgcgagc ggtgttacag cccgtgagca gcgtgctggc aagcaaaaca
     8041 agaacgataa ggcgtttcat catctctgac ctgaataagt gatggtgcag cctgcaggcc
     8101 gcaccacaac cgcattactt cacgcgagag acgtattcac cagagcgggt atccactttg
     8161 atgacttcgc cgatttgtac gaacagcgga actttaacca cagcgccagt agacagggta
     8221 gccggtttgc cgccagtacc tgcggtatca cctttcaggc ccggatcggt atcaacgatt
     8281 tccagttcaa cgaagttcgg cggagtaacg gagatcggct gaccattcca cagagttacg
     8341 atacactctg cctgatccag cagccattta gcgttgtcgc caattgcttt tgcgtcagca
     8401 gacagctgct cgaaagtttc gttgttcatg aagtgccaga actcaccgtc gttgtacagg
     8461 taagtcaggt tcatatcgac aacatcagcg ccttcagcgg aatcagtaga tttgaaggtt
     8521 ttttctacgc gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct
     8581 ttacccggtt ttacgaattc actcgcttca accgcgtaag gttcgccgtc taacatgatt
     8641 ttaagaccag cacgaaaatc gttgctatag tacgttgcca taaggccctc tgaaatttgt
     8701 taattggtag ctaagccaca aaatggcgca tattgtaacc ctaaataccc catccagaga
     8761 agattggtta acgcaacttg ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt
     8821 gaatatagac gcggacgaaa aactgttggc cggacgcaac gccaaaaagc tgtttgccct
     8881 gcgtgtgccc cgctcattta tcgatcgcat ggagaaaggc aatccggacg atccgctttt
     8941 gcgtcaggta cttacctcgc aagatgagtt tgccatcgcg cccggattct ccaccgaccc
     9001 gctggaagaa cagcacagcg tagtgcctgg tttgttgcat aaataccaca accgggcgct
     9061 tttgctggtc aaaggcggct gcgcggtaaa ttgccgctat tgcttccgcc gccacttccc
     9121 ctatgccgaa aatcagggca acaagcgtaa ctggcaaact gcacttgagt atgttgctgc
     9181 gcatccggaa ctggacgaga tgattttttc cggcggcgat ccgctgatgg cgaaagatca
     9241 cgagctggac tggttgctca cacaactgga agccatcccg catatcaaac gtctgcggat
     9301 tcacagtcgt ctgccgattg tgatcccggc acgcatcaca gatgcgctgg ttgaacgctt
     9361 ttcacattct acgctgcaaa tcttgctggt gaatcacatc aaccatgcca atgagataga
     9421 tgaaacattc cgtcaggcga tggctaagtt gcgccgggcg ggcgttactt tgctgaacca
     9481 gagcgttctg ttacgtggtg tgaacgataa cgcacaaacg ctggcaaacc taagtaatgc
     9541 gttgttcgat gccggcgtga tgccgtatta cctgcatgtg ctcgataaag tacagggcgc
     9601 ggcgcatttt atggtgagtg atgacgaagc acggcagatt atgcgtgagt tgctgacact
     9661 ggtgtcggga tatctggtgc cgaaactggc gcgagaaatt ggcggcgaac ccagcaaaac
     9721 gccgctggat ctccagctac gccagcagta actgattaaa cagggaagtc aaacattaaa
     9781 attgcctgat gcgctacgct tatcaggcct acatcgtgca ttgaaattga atggatttgc
     9841 aatgaactgt aggccggata aggcgtttac gccgcatccg gcaagagcaa caaacgcaag
     9901 tacagcaacc tgtcattaaa ctgggaaatt taatctttgc ccgttgcata acctttaatt
     9961 attcatatgc attgctgttg ttgctcttta tataactaac atcaataatt aacctttctt
    10021 ttctgtttat atagagtgag ta
//
LOCUS       DAZUCE010000189         9915 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZUCE010000189 DAZUCE010000000
VERSION     DAZUCE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.189"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            226..1875
                     /locus_tag="ACHY03_003920"
     CDS             226..1875
                     /locus_tag="ACHY03_003920"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFS3907098.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(1926..2528)
                     /locus_tag="ACHY03_003921"
     CDS             complement(1926..2528)
                     /locus_tag="ACHY03_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFS3907099.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            3048..3947
                     /locus_tag="ACHY03_003922"
     CDS             3048..3947
                     /locus_tag="ACHY03_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3907100.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQNPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            3996..5009
                     /gene="yhjD"
                     /locus_tag="ACHY03_003923"
     CDS             3996..5009
                     /gene="yhjD"
                     /locus_tag="ACHY03_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFS3907101.1"
                     /translation="MTQENEIKRPTQDLEHEPIKPLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            5421..6743
                     /locus_tag="ACHY03_003924"
     CDS             5421..6743
                     /locus_tag="ACHY03_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3907102.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(6925..8985)
                     /locus_tag="ACHY03_003925"
     CDS             complement(6925..8985)
                     /locus_tag="ACHY03_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFS3907103.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGKGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDSPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(9055..9822)
                     /gene="pdeH"
                     /locus_tag="ACHY03_003926"
     CDS             complement(9055..9822)
                     /gene="pdeH"
                     /locus_tag="ACHY03_003926"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFS3907104.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQINLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
BASE COUNT         2348 a         2676 c         2428 g         2463 t
ORIGIN      
        1 aatggcatat catgattgcg gcgagagcag gattgaatgt tctgaaaatg aaaaacaaca
       61 caaaatctgt atttttataa atttcatgga tattagtgcg ttatattttt tgacaggtta
      121 ataaacgtga tccaccgcac gctttgtcgc ccaccaggcg gagcgaatga ctacccttaa
      181 agaaaagccc gataattagc gacgaatttc ggaggttgga tccttatgct caatcagaaa
      241 attcaaaacc ctaatccaga cgaactgatg atcgaggtcg atctctgcta tgaactggac
      301 ccgtatgaat taaaactgga tgagatgatc gaggcagaac cggaacccga gatgattgaa
      361 gggctgcccg cctctgatgc gctgacgcct gccgatcgct atctcgaact gttcgagcat
      421 gttcagtcgg cgaaaatttt ccccgacagt aaaacctttc ccgactgcgc acccaaaatg
      481 gacccgctgg atattttaat ccgctaccgt aaagtgcgcc gtcatcgtga ttttgacttg
      541 cgcaagtttg ttgaaaatca cttctggctg ccggaggtct actccagcga gtatgtatcg
      601 gacccgcaaa attccctgaa agagcatatc gaccagttgt ggccggtgct aacccgcgaa
      661 ccacaggatc acattccgtg gtcttctctg ctggcgctac cgcagtcata tattgtcccg
      721 ggcggccgtt ttagcgaaac ctactattgg gattcctatt tcaccatgct ggggctggcg
      781 gaaagtggtc gggaagattt gctgaaatgc atggccgata acttcgcctg gatgatcgaa
      841 aactatggtc acatccccaa cggcaaccgc acctattatt tgagccgatc gcaaccaccg
      901 gtttttgcgc tgatggtgga gttgtttgaa gaagatggtg tacgcggtgc gcgccgctat
      961 ctcgaccacc tgaaaatgga atatgccttc tggatggacg gtgcagaatc gttgatccct
     1021 aatcaggcct atcgccatgt tgtgcggatg ccggacggat cgctgctcaa ccgttactgg
     1081 gacgatcgcg acacgccgcg tgacgaatcc tggcttgagg acgttgaaac cgcgaaacat
     1141 tctggtcgcc cgcccaacga ggtgtaccgc gatttacgcg cgggggcggc ctccggttgg
     1201 gattactctt cccgttggct gcgtgatact ggtcgtctgg cgagcattcg taccacccag
     1261 ttcatcccca tcgatctgaa tgccttcctg tttaaactgg agagcgccat cgccaacatc
     1321 tcggcgctga aaggtgagaa agagacagaa gcactgttcc gccagaaggc cagtgcccgt
     1381 cgcgatgcgg taaaccgtta cctctgggat gatgaaaacg gcatctaccg cgattacgac
     1441 tggcgacgcg aacaactggc gctgttttcc gctgccgcca ttgtgccact ctatgtcggt
     1501 atggcgaacc atgaacaggc cgatcgtctg gcaaacgccg tgcgcagccg gttactgaca
     1561 cctggcggga ttctggcaag cgagtacgaa accggtgaac agtgggataa acccaacggc
     1621 tgggcaccgt tacagtggat ggcaattcag ggatttaaaa tgtatggcga tgaccttctg
     1681 ggtgatgaaa tcgcgcgaag ctggctgaag acggtgaatc agttctatct ggaacagcac
     1741 aaactgatcg aaaaatacca tattgccgat ggtgttcccc gcgaaggcgg cggtggcgag
     1801 tatccgttgc aggatgggtt tggctggact aacggtgtgg tacgccgttt aattggtttg
     1861 tacggcgaac cataatattt ttacagccag ccgctaactt cctgctggct gtaaaattat
     1921 cctcttcagg aggagatatt taacatcatt gccgcctggg tgcgattttt cacttccaga
     1981 cggcgataca gggattccag atgcgctttt accgttccgg tactgatatt cagcgctctg
     2041 ccgatctcct tgtttgattc gcccgccgct aacatggtta aaatctcccg ctggcgggcg
     2101 cttaacgatt tgagatcttt aatgtccttt tccggcgtcg tccgccagtc tccaggcaga
     2161 aacatcatcc ccatcgccgc actatttacc gccaacgcaa atgtctcgac ggttgaatca
     2221 cgaggcacaa tggccagcac attaaaatgg ataacttcct gtaaccaccg tttattgcaa
     2281 tccgtcgccg taattaacac cttaacctca ggaaattgca ccacggtttt ttgcagcagc
     2341 cagtagcaaa actcaccatc ctgatcgcca tcgagcataa ctaaggcttc agggtaactt
     2401 tccagctttt gccataactc gtctgcctga ctggccccct gaatactcac tcctggaata
     2461 cgctgctgta aactgatttt cattccatga ataaatattg actgcctgtc aaacatgact
     2521 atttgcataa ctgaatctcc acctgaatat gttaaaaaga cttaagtagt ggaagggtat
     2581 tacccgcgag aaaaaataag aattcgccat ttggcggtgg ccattctaca gagatgacgc
     2641 gtagaaaata gttaccgata taaatagtta cagctaaacg cctgaaatta catgtcgagg
     2701 gcactattta aaacaatttt gaggatttcc ttatattgat ggttagtacg catgcaatta
     2761 aaaataaaat tccgcgacca caagccaaaa taacaaacgg caatgagaca aaaataagca
     2821 caaatagcca atacgtcctc tattcacttt aaagggaatc gctgaaaaat acgctctgtt
     2881 taaggggatt cacctttctc agaaagctat tccgcccttt tcctgctgag aaatcgccac
     2941 attcgtcatg acaacattgt gaaacccggc attagatgtt agaaaaaaca ataaacaatg
     3001 cgatatgcgc gttactgttt cgctgtgaac aacaatcggt caaagaaatg gataaaattc
     3061 acgcaatgca gttgttcatc aaagtcgcgg agctggaaag tttttcccgc gcagcggatt
     3121 tctttgcttt gccaaaggga agtgtttcgc gccagataca ggcactggaa catcaacttg
     3181 gcacccagct tctccagcgc accacgcgac gggtcaaact cacgccagaa ggcatgacct
     3241 attatcaacg ggcaaaagat gtgttgagta atctcaacga actggacggt ctgtttcaac
     3301 aggatgccac cagtatcagc ggtaaattac gcatcgacat cccgccagga atcgcgaaaa
     3361 gcctgttact gccgcgcctg tcggaatttc tctatctgca tccgggaatt gagctggaac
     3421 tgagtagcca tgaccgtccg gtagatattc ttcatgatgg ttttgattgc gtgatacgca
     3481 ctggcgcgtt accggaagat ggcgttatcg cccgtcccct cggcaaactg accatggtca
     3541 actgtgccag tccgcactat ctgacgcgct ttggttatcc gcaaaacccc gatgatctga
     3601 cttcacacgc aatagtgcgt tatacaccgc acctgggtgt acatccgtta ggttttgagg
     3661 ttgccagcgt taatggcgtc cagtggttta agtctggcgg catgttgacg gtaaacagta
     3721 gcgaaaacta tctcgccgcc ggtcttgccg gtctggggat tattcagatc ccgcgcattg
     3781 ccgtgcgcga agccctgcgt gccgggcggc ttattgaagt gttgccaggc taccgtgccg
     3841 agccgctctc cctttcgctg gtttatccgc agcgtcggga gctttcccgg cgtgtaaacc
     3901 tgtttatgca gtggctggct ggcgtaatga aagagtacct ggactgaccg actatacttt
     3961 ttaagaatga ccacgacaga aggacaaaag agcggatgac gcaggaaaac gagatcaaac
     4021 gtcccaccca ggatctggag cacgagccga ttaagccgtt ggataatagc gagaaaggcg
     4081 gcaaagttag ccaggcgctg gaaaccgtca ccaccactgc cgaaaaagtc caacgccaac
     4141 cggttattgc gcacctgatt cgcgcgacag aacgctttaa cgatcggctg ggtaaccagt
     4201 ttggtgcggc tatcacctat ttctcatttt tatcgatgat cccgattttg atggtgtcgt
     4261 ttgccgcagg gggctttgtg ctggcctccc atccgatgtt gctacaggat atcttcgaca
     4321 aaattctgca aaacatcagc gatccgacgc tagccgctac gttgaaaaac accatcaaca
     4381 ccgccgttca gcagcgtacg actgtagggc ttgtcggcct ggcggtggcg ctttattccg
     4441 gcatcaactg gatgggtaac ctgcgtgaag cgattcgcgc ccagtcgcgc gatgtctggg
     4501 aacgctcgcc gcaagatcag gagaagttct gggtaaaata tctgcgtgat tttatttcgc
     4561 tgattggttt gttgattgcg ctgattgtga cgctttcgat cacctcggtt gccggttcgg
     4621 cgcagcaaat gattattagc gccctgcacc tgaacagcat tgagtggctg aaaccgacgt
     4681 ggcggttgat tggcctggcg atttccatct tcgccaacta tctgcttttc ttctggatct
     4741 tctggcggtt gccgcgccac cgtccgcgca aaaaagccct gattcgcgga acatttctgg
     4801 ctgctattgg ttttgaagtg attaaaatcg tgatgaccta caccctgcca tcgttgatga
     4861 aatccccctc tggtgcagca tttggttccg tgctggggct gatggcattt ttctacttct
     4921 tcgcccgttt gacgctgttt tgcgcggcgt ggattgccac cgccgaatat aaagacgacc
     4981 cgcgaatgcc ggggaaaacg cagccttaaa acaggccggg tgcagttcaa aacgccttat
     5041 ccggcatacg aatttattcg gcctggctcc ccgtaggccg aataagatgc gccagcatcg
     5101 catccggcta taatgcgcac ataacctctt gaaactcttc cccagagcct cttcagccat
     5161 ctatttggga gcaaacaatt tcatgccaac tcataacccc agcatataaa tccagctggt
     5221 aacttttatt taacctgaaa ccagttttat ccaccattta taaaattatg tgaagcattt
     5281 catagaagaa aaatcactgg cctaaacatt atcccctttt tgccttattt ttgaccactt
     5341 ccgcgatttg ttacacattg aaatatcact tttgctgtgc gtaatatggc tattcgttag
     5401 ccaaaaaata agaaaagatt atgcaagcaa cagccacaac actcgaccac gagcaagaat
     5461 acacgccgat caactcgcgt aataaagtcc ttgtcgcctc tctcattggc acggccattg
     5521 agttcttcga cttttacatt tacgccactg cggccgttat tgtgtttccg catatcttct
     5581 tcccgcaggg cgatccaacg gcagcaacgc tacagtcgct cgccaccttc gccatcgcct
     5641 tcgtcgcgcg ccccattggc tctgccgtat ttggtcattt tggcgatcgc gttgggcgta
     5701 aagcgacgct ggtcgcctcg ttgctaacga tggggatttc gaccgtggtg attggtctgc
     5761 tgccgggcta tgccacgatt ggtattttcg ccccgctgct gctggcgctg gctcgatttg
     5821 gtcagggtct gggcttaggc ggtgaatggg gcggcgcggc gctgctggcg actgaaaacg
     5881 ccccaccgcg caaacgtgct ctgtatggct ccttcccaca gctgggcgca ccgattggct
     5941 tcttctttgc caacggcact ttcttgctgc tttcctggct actgaccgac gagcagttta
     6001 tgagctgggg ctggcgtgtg ccatttatct tctcggcggt gctggtcatt atcggcctgt
     6061 atgttcgcgt gtcgctgcat gagtcgccgg tgtttgagaa agtcgctaaa gcgaaaaaac
     6121 aggtgaagat cccgctgggt acgctgctga ccaaacatgt tcgcgtcacg gtgctcggta
     6181 ccttcatcat gctggcaacc tatacgctgt tttacatcat gacggtctac tctatgacct
     6241 tcagtaccgc cgccgcgcca gttgggcttg gcctgccgcg taacgaagtg ttgtggatgc
     6301 tgatgatggc agttattggt tttggcgtga tggtgccagt cgccggatta ctggctgatg
     6361 cctttggtcg ccgtaaaagc atggtaatta tcaccacgct gatcatcctg ttcgcgctgt
     6421 tcgcctttaa cccgctgctc ggttctggca acccgattct ggtttttgcc ttcctgctgc
     6481 tggggttaag tctgatgggg ctgaccttcg ggccgatggg cgcgctgtta ccagagctgt
     6541 tcccaacaga agtgcgttac accggagcat cattctctta caacgtagcg tcgattctcg
     6601 gggcttccgt tgcgccgtat atcgcagcct ggttgcagac taactacggg ttaggtgcgg
     6661 tggggttata tctggcggcg atggctggct tgacgttaat cgccctgctg ctgacccatg
     6721 agacgcgaca tcagtcgttg taataagagt gaaaagcctg atgcgctacg cttatcaggc
     6781 ctgaatttcc atacaatata ttgaattcac aaggatttgt aggccggata aggcgttaac
     6841 gccgcatccg gcatgaacaa agcacacttt gccaacaatc taaatctgtt taattgccgg
     6901 atgtcagaca tccggcaatt atcatcactt cttcatctgc gacaaaatcg tccgacactg
     6961 attagcctcc ccttcggaag gtgagatcag cgccagtaac gccgccgccg gtgttaccag
     7021 cgttgccagt gccgcagcaa cagcaccacg ggcaatcaac ggcccggctt tcaccccagc
     7081 ctgaggattt ttaaacgtcc cacgcacata cagcggcgaa cgcagtgtga taatccgaat
     7141 tcctttactc tccggatcaa tagtcaaatc cagctgttcc gaagcaaaac ttgccgtgcc
     7201 ggtaacgtta atcaacgcgt tctcagtatc gaaagcaaag atctgcgggc gcgccacgcc
     7261 gttggcaata tccagattcg ccgccgcgca gttcacccgc acctcatcat caccaaatat
     7321 cgcaccgaca atgtagttgc cgacattcag cccaacaatc tccatcaggt tgcggctcac
     7381 cagcccgtca ttcatcaaca gtttcagatt gccgttactg ttgcctaaaa gtgccgccac
     7441 cgagttaccg ctgccgcgca gttccgcgtc accgttcatt tcccccagcg tcttctgcat
     7501 cagttccaca tcgggcatca gttctttcag tttcagtcga cgagcctgaa tatctgcccg
     7561 cccctgcatc ggctttttat cgccttccag atgaatattc gccgcaatgc tgccgcccgc
     7621 catgccaaat ttcagcggtt gcaggcgcag gtcagcattt ttgaggatga tatgagtaga
     7681 aagatcgcta atcggcaggc tactgccatg ctcgatacgt cggcctttga aacgaacatc
     7741 ggcatccatg acgtcccatt tatcggtttc gaagcggtca taaggcagca ctttacccgc
     7801 aggctgaacg cttttttcgc ccttcttctg ttcagaccgt ttcgactttt ctgccccttt
     7861 cccggaatca acgccaatca acggtcccag gtccgccagc cgcaattgcc gcgactcgac
     7921 atcaccttcc agttttggtc gtggcttgcc ggtggtgtag accagagaac cgtggatatc
     7981 gctatcgcca attcgcccat taaagccgcg ataatcaaag accgacgatt tttcagtgtc
     8041 gattttcgct accagccgac catccgtttc aaacggcggg gaatcgggca gcagaacgcc
     8101 cgtcagttca tagagatcac ccagtgaatc gccagaaaat ttaagccgta aatcgacacc
     8161 gcccatcttc attgggtcat tcacgacgcc atcaaaagca acacgggtat tacctgaacg
     8221 gaaatcagcc tgtaccggaa acggcgtccc ttcgccacgc agcgccagca taccgccaat
     8281 tttcccctta cccgtgagcg gttcaccgtt ataacgtccc tgcgccttca ggccaaaaac
     8341 gtaatcgccg accttttctt tatccgcttt acctttcgat ccagtaactt cgctgaacgg
     8401 cagcggcttg cctaacggat cgacaaaaat ttccagatcc gctttgctta ctttgtcatc
     8461 aatagcgatc cgcccttgat cgaacagaat attatccagc cgaaacgacc atgccgacgg
     8521 ctttgcattc gcgtctttgt tgtcatcgtt ggcaagatta aacgtccagt tattgttctt
     8581 ttcagagagg cgaatcaggc gtgcgtcggg cttttccagt ttgatccacg gtagccagac
     8641 cgttttggtc agcagcgcca acggtgccag cgttgcctct actcgcggca aatgcaccat
     8701 cgtgacttcg ggaatatccg gtggattgcc aagaatgatg tcttccgcat gcacatgagg
     8761 ccacggcacc cagctgcgcc agccagtttc ttgtttttgc cgctcccaca ccacgcccag
     8821 atcgccacgg atagcgaacg gacgattcaa ctccgcagag actttctggt tgatggtcgg
     8881 tttgagtcga ttccagtcaa aagttgcaat caaaatgatc gccacgacaa tcaacaacaa
     8941 gaaagcccct gaaatcgcag cggttatttt gcctgccttg ctcatcgttc gcttcctcat
     9001 attcttcctg tgccagtcct aaagatagtc cagccaggcg gaaaatgagg cagcttatag
     9061 cgccagaacc gccgtattca gcgtttctat cggtgccggg cgtgaaagaa accagccttg
     9121 tgcggcgaat gcgggcgagt tctgaacatc acgccactct tccggcgttt ctacgccctc
     9181 aacaatcacc ccgcgacaat agcgattcat tagatgtaaa agctgagaaa agagtgtgcg
     9241 tccttccggt gactgacgca gcatcacaaa cagttctcgc gcgattttga tgtagtcata
     9301 acgcacttca cttagtgcag agaaatttgc catcccggta ccaaaatcat ccagccacag
     9361 cgggccaaat tcacacatcg aggcaaaggt tgaatctttc ggcagacgga tatgctccac
     9421 cagttcgaaa cgcagccagg gaagacgctc aatctggcgc aggatttttg gttgctgacg
     9481 cagggcgatg agcgtagggc catcaatatt gaccgatgcc agcaggccgt gctctacaaa
     9541 gaagtcggct ttttgcgcca gcaaattaat ctgctctttc acaacctcca tccgatggct
     9601 gacggtgatt tcagtaaaat agcgatccgg cggcaggcgt tgcgaagggt tcaagggatg
     9661 cgtgaccacc gttaatagct ccacggccat taaccgcccg catgtttgat agatcggctg
     9721 ccaggtgtaa gcacgctcac actgcaacca aaaacgccgt tcctgcaagc tctcgatgct
     9781 tgcttcaggg ttgcttattc gctggataac ctgccttatc atcaaagatg tcctgtgttt
     9841 aaaagtgatg ctgcccggac tcgacaaaga ttatcggcgc gtaagggcag aactttaccg
     9901 cctggccggg gtaaa
//
LOCUS       DAZUCE010000190         9866 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZUCE010000190 DAZUCE010000000
VERSION     DAZUCE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.190"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            186..689
                     /gene="dps"
                     /locus_tag="ACHY03_003927"
                     /gene_synonym="pexB"
     CDS             186..689
                     /gene="dps"
                     /locus_tag="ACHY03_003927"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFS3907105.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1093..1839
                     /gene="glnH"
                     /locus_tag="ACHY03_003928"
     CDS             1093..1839
                     /gene="glnH"
                     /locus_tag="ACHY03_003928"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFS3907106.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            1978..2637
                     /gene="glnP"
                     /locus_tag="ACHY03_003929"
     CDS             1978..2637
                     /gene="glnP"
                     /locus_tag="ACHY03_003929"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFS3907107.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2634..3356
                     /gene="glnQ"
                     /locus_tag="ACHY03_003930"
     CDS             2634..3356
                     /gene="glnQ"
                     /locus_tag="ACHY03_003930"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFS3907108.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKIPPSQRLQEFLQHVS"
     gene            3617..5842
                     /gene="ybiO"
                     /locus_tag="ACHY03_003931"
     CDS             3617..5842
                     /gene="ybiO"
                     /locus_tag="ACHY03_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFS3907109.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(5839..6765)
                     /gene="rlmF"
                     /locus_tag="ACHY03_003932"
     CDS             complement(5839..6765)
                     /gene="rlmF"
                     /locus_tag="ACHY03_003932"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFS3907110.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     VNASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            7041..7301
                     /gene="mcbA"
                     /locus_tag="ACHY03_003933"
     CDS             7041..7301
                     /gene="mcbA"
                     /locus_tag="ACHY03_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFS3907111.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7566..9848
                     /locus_tag="ACHY03_003934"
     CDS             7566..9848
                     /locus_tag="ACHY03_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFS3907112.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGIAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTATLNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTASIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     SPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
BASE COUNT         2411 a         2573 c         2481 g         2401 t
ORIGIN      
        1 gcgagcaaat aaataagaat tgttcttatc aatatatcta actcattgaa tctttattag
       61 ttttgttttt cacgcttgtt accactatta gtgtgatagg aacagccaga atagcggaac
      121 acatagccgg tgctatactt agtctcgtta attactggga cataacatca agaggatatg
      181 aaattatgag taccgctaaa ttagttaaat caaaagcgac caatctgctt tatacccgca
      241 acgatgtctc tgacagcgag aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc
      301 agtttattga tctttctttg attaccaaac aagcgcactg gaacatgcgc ggcgctaact
      361 tcattgccgt acatgaaatg ctggatggct tccgcactgc actgatcgat catctggata
      421 ccatggcaga acgtgccgtg cagctaggcg gtgtagctct ggggaccact caagttatca
      481 acagcaaaac cccgctgaaa agttacccgc tggacatcca caacgttcag gatcacctga
      541 aagaactggc tgaccgttac gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag
      601 cgaaagatga cgacaccgca gatatcctga ccgccgcgtc tcgcgacctg gataaattcc
      661 tgtggtttat cgagtctaac atcgaataaa tccatcgctg atggtgcaga actttagtac
      721 acggtaaaag cggcttcctg acaggaggcc gttttgtttt gcagcccgcc tcaacgcact
      781 tatttagtgc atccatctgc tatccccagc tgattaagta aattttttgt atccacatca
      841 tcacacaatc gttacataaa gattgttttt tcatcaggtt ttacgctaaa taatcactgt
      901 gttgagtgca caattttagc gcaccagttt ggtgccccag aatggtgcat cttcagggta
      961 ttgccctata aatcgtgcat cacgtttttg ccgcatctcg aaaaatcaat gagttgcaaa
     1021 actggcacga ttttttcata tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa
     1081 aaaggaaatg ctatgaagtc tgtattaaaa gtttcactgg ctgcactgac cctggctttt
     1141 gcggtttctt ctcatgccgc ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt
     1201 ccgtttgaat ttaaacaggg cgataaatat gtgggctttg acgttgatct gtgggctgcc
     1261 atcgctaaag agctgaagct ggattacgaa ctgaagccga tggatttcag tgggatcatt
     1321 ccggcactgc aaaccaaaaa cgtcgatctg gcgctggcgg gcattaccat caccgacgag
     1381 cgtaaaaaag cgatcgattt ctctgacggc tactacaaaa gcggcctgtt agtgatggtg
     1441 aaagctaaca ataacgatgt gaaaagcgtg aaagatctcg acgggaaagt ggttgctgtg
     1501 aagagcggta ctggctccgt tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt
     1561 cagttcccga acatcgataa cgcctatatg gaactgggca ccaaccgcgc agacgccgtt
     1621 ctgcacgata cgccaaacat tctgtacttc atcaaaaccg ccggtaacgg tcagttcaaa
     1681 gcggtaggtg actctctgga agcgcagcaa tacggtattg cgttcccgaa aggtagcgac
     1741 gagctgcgtg acaaagtcaa cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac
     1801 gaaatctaca aaaaatggtt cggtactgaa ccgaaataat aacgctacac ctgtaaagcg
     1861 cactggcagt tccctctccc ctctggggag aggattaggg tgaggggcgc aaacccgctc
     1921 cggggccatt aattaccctg aatttgatta tttacaccac ggtaacagga acaacatatg
     1981 cagtttgact ggagtgccat ctggcctgcc attccgcttc tgattgaagg tgccaaaatg
     2041 accctgtgga tttcggtcct cggtctggct ggcggtctgg taatcggatt gctggcaggt
     2101 tttgcacgca ccttcggtgg ttggatagcc aaccacgtcg cgctggtctt tattgaagtg
     2161 atccgcggca cacctatcgt cgtccaggtg atgtttatct attttgccct gccgatggcg
     2221 tttaacgact tacgcatcga cccatttact gcggcggtgg tcaccatcat gatcaactcc
     2281 ggcgcgtaca ttgcggaaat cacgcgtggt gcggtgctgt ctatccacaa aggttttcgt
     2341 gaagcaggac tggcgctcgg tctttcacgt tgggaaacca ttcgctacgt cattttaccg
     2401 ctggcactgc gtcgtatgct gccgccgctg ggtaaccagt ggatcatcag cattaaagac
     2461 acctcgctgt ttattgttat cggcgtggcg gaactgaccc gtcaggggca agaaattatt
     2521 gccggtaact tccgcgccct tgagatctgg agcgccgtgg cggtgttcta tctgattatt
     2581 accctggtgc tgagctttat tctgcgtcgt ctggaaagaa ggatgaaaat cctgtgattg
     2641 aatttaaaaa cgtctccaag cactttggcc caacccaggt gctgcacaat atcgatttga
     2701 acattgccca gggcgaagtc gtggtgatta tcgggccgtc cggttccggt aaatcgaccc
     2761 tgctgcgctg catcaacaaa ctggaagaaa tcacctccgg cgatctgatt gtcgatggcc
     2821 tgaaggttaa cgatccgaaa gttgacgagc gcctgattcg ccaggaagca ggtatggtgt
     2881 tccagcagtt ttacctcttc ccgcatctga cggcgctgga aaacgtcatg tttggcccgc
     2941 tacgcgtgcg tggcgcgaac aaagaagagg cggaaaaact ggcgcgtgag ctgctggcga
     3001 aagtcggtct ggcagaacgt gcacatcact acccttccga actttctggt ggtcaacagc
     3061 agcgtgtggc gattgcccgc gcgctggcgg tgaagccgaa aatgatgctg tttgatgaac
     3121 cgacttccgc tcttgacccg gaactgcgcc atgaagtgct gaaggttatg caagatctgg
     3181 ctgaagaagg gatgacgatg gtgatcgtga cccacgaaat cggttttgcc gagaaagtag
     3241 cttcgcgcct gatctttatc gacaaaggcc ggattgcgga agatggcgat ccacaagtgt
     3301 tgatcaaaat tccgccgagc cagcgcttgc aggaattttt acagcacgtc tcttaataag
     3361 acacattgcc tgatgcgcta cgcttatcag gcctacagga tatctggcaa cttattaaaa
     3421 ttgcatgaac ttgtaggacg gataaggcgt tcacgccgca tccggcaaaa aagcccgcac
     3481 gttgtcagca acctgcttaa tatcccttcc tccctttcac ccgaaaggga ggcacaccag
     3541 attcctctca tttaaaatcg ccccttcccc agcatctata cttatctttt tgctctgttt
     3601 tctcactgga ggagtcatgc ggtggatcct gttcatcctc ttctgcctgc tgggcgcacc
     3661 tgcccacgcg gtatccatac ccggcgttac aaccacaacg acaacggact caacgactga
     3721 accggccccg gaaccggata tcgaacaaaa aaaagcggcc tatggcgcac tggcggatgt
     3781 gctggataat gacacctcgc gtaaagagtt gatcgaccag ttgcgcaccg tcgccgcgac
     3841 gccacctgct gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt
     3901 gctgcaaaag gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg
     3961 gcagctttat cgcaatatca ccggctcccc gcataagccg tttaatccac aaaccttcag
     4021 caatgcgctg acccattttt caatgttagc ggttttagtg tttggttttt actggctgat
     4081 tcgcctgtgc gcactgccgc tgtatcgcaa aatgggtcag tgggcgcggc aaaaaaatcg
     4141 tgagcgcagt aactggctgc agcttccggc gatgattatc ggggcgttta ttatcgacct
     4201 gctgttactg gcactgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg
     4261 cagtcgcacc atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt
     4321 tttcaaagct gtactacgcc tgattttttg cccaaatgtg gcggagctgc gcccgttcac
     4381 gatacaggac gagagcgccc gttactggag tcgtcgcctg agctggttaa gcagtttgat
     4441 aggttatggc ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg
     4501 tgcgctggcg aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt
     4561 tcgtaataaa aaagagatta cccagcattt gctcaacttc gcggagcatt cgctggcctt
     4621 tttcagcctg tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt
     4681 tatcgtgctg tttttctttt cgttgttcga tccgggcaac agcctgaaat ttatgatggg
     4741 tgcaacagtg cgcagcctgg cgattattgg tatcgcagca tttgtttccg gtatgttttc
     4801 ccgctggctg gcaaaaacca tcactctctc gccacatact cagcgtaact atccggagtt
     4861 gcaaaaacgg ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt
     4921 ctgcgtggct gtaatgctgc tgttgagcgc atggggattg ttcgatttct ggaactggct
     4981 gcaaaacggc gcggggcaga aaaccgtaga tatcctgatc cgtatcgcac tcattctttt
     5041 cttctcggcg gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga
     5101 tattcatggc cgcccgctac ccagcgcccg cacgcgtacc ctgcttacgc tgtttcgtaa
     5161 cgcgctggcg gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt
     5221 caatatcgcg ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc
     5281 gcaaacgctg gtgaaagata ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa
     5341 cactggagat ttggtgacta tcgggccgtt gaccggcact gtggaacgga tgtcgattcg
     5401 ctccgtgggc gtacgccagg ataccggggc gtaccacatc attccgtggt cttcgattac
     5461 cacctttgcc aactttgtcc gcggcattgg ttcggtagtg gcaaactacg atgttgatcg
     5521 ccatgaagat gctgataaag ccaatcaggc actgaaagat gcggtagcgg aattaatgga
     5581 aaacgaagaa attcgcgggc tgattattgg tgaaccgaac tttgccggga ttgtcggctt
     5641 aagcaatacc gcgtttacac tgcgtgtttc gttcaccacg ctgccactca aacagtggac
     5701 ggtacgcttt gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc
     5761 gccagtgcag acttatcagg tgctgcctgc tccgggcgcg accccggctg aaccgttacc
     5821 gccgggggag ccaacgcttt aacgctggcg attgacaaaa cggcggcgct gctcgtcgtt
     5881 cataaaggtc caggcaataa agcgactctg cttttgcccc tgggccatct cttttttaac
     5941 caccttcacc gcgcccacgt ccgtcagggc acgatacaac ggcggtaagt tttcaccacg
     6001 agataccagt gaagtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat
     6061 ctttttgata aaggtgactt caccgccttc acaccacaac tcctgttgct ggccaccaaa
     6121 gttcagtgca tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg
     6181 cgccgcagcg gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt
     6241 tttatggatg attccgttaa aaatcgcccc gctctctttt tgccgacgca ggcgaatggc
     6301 gcgattaagc cccggattag cactgatgat cgcctgcgcg ctgcttaacg cctggctgct
     6361 ggtttcacta ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca
     6421 gttcgcacca acgccgatat ccagaatgct ggcattaact ggaattgttc cgcttgcttc
     6481 tgccagtaaa tcggcaaggt gatgaatata atccgcccgg cccggtactg gcggacagag
     6541 aaaaccgtcc gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt
     6601 gagcgccttc accgccagcg gattggcaaa gtctacgctt tgctccccgg cgggtgtaag
     6661 cgtgaggaat tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct
     6721 gtgatgacgg ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa
     6781 gcagcggcgt aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt
     6841 tctaaaaacc ttaacatggc tatggtttat ggcaattcat cataaaaatc agcatcatgc
     6901 atgtcattga tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag cgtatctctg
     6961 tcaatttttt gcgtcatttt catcgcccag gcgacctaca cttatcaggc actacccgtt
     7021 gtatttatga ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga
     7081 gcggaatctc tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc
     7141 tacggcccgc cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata
     7201 aactggcaga aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg
     7261 gaaatgacca gatgttcggt actgcaacca tctacaaata accccaccaa cgtaactgca
     7321 ttcgcctctg gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaacgca
     7381 atgtaaataa atgtatttct ttttcgcgca atgggtgata gaaaatcgcg ccaagtgata
     7441 atgcttatca aaattattat cacttgcacg aacactatca cgagattaac agtggcatcg
     7501 catccgcaga gaggctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct
     7561 gcaaaatgga aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttctttg
     7621 ccggtctttg tattggcatc acgcctgtgg ctcaggcact cgccgccgaa gggcaaacta
     7681 acgcggatga cacgctggtt gtcgaagcat caacgccttc gctttatgcg ccacaacaat
     7741 ctgcagatcc gaaattctcg cgtccggtag cggatactac ccgcacgatg acggtgattt
     7801 ctgaacaagt gattaaagat cagggcgcaa ccaaccttac cgacgcactc aaaaacgtcc
     7861 ccggcgtggg tgcgtttttt gcgggtgaga acggtaactc caccactggc gacgccattt
     7921 atatgcgcgg tgctgataca tctaacagta tttatattga tggcattcgc gatatcggca
     7981 gcgtctcgcg cgacaccttc aataccgagc aggtcgaagt gattaaaggg ccttccggca
     8041 ccgactacgg gcgcagcgcg ccgacgggct cgatcaatat gatcagcaag cagccgcgca
     8101 atgattccgg cattgacgcc tccgccagta ttggcagcgc ctggttccgc cgcggcacgc
     8161 tggacgtcaa tcaggtcatt ggtgatacca ccgcggtgcg cctgaatgtg atgggcgaaa
     8221 aaacgcacga tgccggacgc gacaaagtca aaaatgagcg ttacggcatc gccccttctg
     8281 tcgcttttgg ccttggtaca gcgaatcgtt tgtatcttaa ttatctgcat gtcacccagc
     8341 acaacacgcc agacggcggc attccaacca tcggtttgcc gggctattct gccccatctg
     8401 caggaacggc gaccctgaat cattccggaa aagttgatac gcataacttt tacggcacgg
     8461 attccgatta cgacgattcg accaccgaca ccgctaccat gcgttttgag cacgacatca
     8521 acgataacac caccattcgc aacactaccc gttggtcgcg cgtgaagcag gattacctga
     8581 tgacggcgat tatgggtggg gcgtcgaata ttacccagcc caccagcgat gtgaatagct
     8641 ggacctggtc acgcacggcg aatactaaag atgtgagtaa taaaattctc accaaccaga
     8701 ccaacctgac ctcgacgttc tataccgctt ctatcggtca tgatgtcagt accggcgtgg
     8761 aatttacccg tgaaacgcag actaactacg gcgttaatcc ggtgacgtta cctgcggtaa
     8821 atatttatca tcctgacagc agcattcatc ccggcggcct gacgcgcaac ggcgcaaacg
     8881 ccaatggtca gacggatacc ttcgcaattt acgccttcga tacgctgcaa atcacccgtg
     8941 attttgagct gaacggcggg atccgtctgg ataattatca tactgaatat gacagtgcca
     9001 ccgcctgcgg cggcagcgga cgcggtgcca tcacctgccc agctggtgtg gcaaaaggtt
     9061 ctccggtcac caccgtcgac accgccaagt cgggcaatct ggtgaactgg aaagccgggg
     9121 cgctgtatca cctgacggaa aatggcaatg tctatattaa ctatgccgtt tcccagcagc
     9181 ctccgggcgg caacaacttc gcccttgcgc agtctggcag cggtaacagt gccaaccgca
     9241 ccgattttaa accgcaaaaa gccaacacca gcgagattgg caccaaatgg caggttctgg
     9301 ataaacgcct gttgctcacc gccgcgctgt tccgtactga tatcgaaaat gaagttgagc
     9361 aaaatgatga cggaacttac tcgcaatatg gtaagaaacg cgtcgaaggc tatgagatat
     9421 ccgtggccgg gaatatcagt cccgcgtggc aggtgattgg cggctatacc cagcaaaaag
     9481 caaccatcaa aaacggcaaa gatgttgccc aggatggttc ctcatcgctg ccctataccc
     9541 cggagcacgc ctttacctta tggagccagt atcaggcaac cgatgatatt tccgttggtg
     9601 cgggcgcacg gtatatcggc agtatgcata aaggttcaga cggcgcggtg ggaacgccag
     9661 catttactga aggttactgg gtcgccgacg ccaaactggg gtatcgggtt aatcgcaatc
     9721 tcgacttcca gctaaacgtc tacaacctgt ttgataccga ttacgtcgcc tcaatcaaca
     9781 agagcggcta ccgttatcac ccgggcgagc caagaacctt cttgctcaca gccaatatgc
     9841 atttctgatt cagatgtggg gcgcag
//
LOCUS       DAZUCE010000191         9756 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZUCE010000191 DAZUCE010000000
VERSION     DAZUCE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.191"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..564)
                     /gene="mocA"
                     /locus_tag="ACHY03_003935"
     CDS             complement(<1..564)
                     /gene="mocA"
                     /locus_tag="ACHY03_003935"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFS3907113.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAFQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKKR"
     gene            667..1437
                     /gene="yqeC"
                     /locus_tag="ACHY03_003936"
     CDS             667..1437
                     /gene="yqeC"
                     /locus_tag="ACHY03_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFS3907114.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQT
                     RGRRVLITTTTHMFMPKSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1485..3110
                     /gene="yqeB"
                     /locus_tag="ACHY03_003937"
     CDS             1485..3110
                     /gene="yqeB"
                     /locus_tag="ACHY03_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFS3907115.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3331..4263)
                     /gene="arcC"
                     /locus_tag="ACHY03_003938"
     CDS             complement(3331..4263)
                     /gene="arcC"
                     /locus_tag="ACHY03_003938"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFS3907116.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVEASQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4311..5696)
                     /gene="hyuA"
                     /locus_tag="ACHY03_003939"
     CDS             complement(4311..5696)
                     /gene="hyuA"
                     /locus_tag="ACHY03_003939"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFS3907117.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLIAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5749..6960)
                     /locus_tag="ACHY03_003940"
     CDS             complement(5749..6960)
                     /locus_tag="ACHY03_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFS3907118.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKTLVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7018..8214)
                     /gene="dpaL"
                     /locus_tag="ACHY03_003941"
     CDS             complement(7018..8214)
                     /gene="dpaL"
                     /locus_tag="ACHY03_003941"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFS3907119.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDRVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8272..9459)
                     /gene="ygeW"
                     /locus_tag="ACHY03_003942"
     CDS             complement(8272..9459)
                     /gene="ygeW"
                     /locus_tag="ACHY03_003942"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFS3907120.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         2286 a         2375 c         2548 g         2547 t
ORIGIN      
        1 ccttttcttc gctgtaataa agtcgtccgg agtatcaata tctaaaatta tttctgcatt
       61 ttctatttcg acggaataat gatctcccat aaggagtgcc tgacgcatat tggtgacatt
      121 gggtcgctgg aatgcctgca tcaagcatgg ttttgacact aaaatcggat ggccggggat
      181 gccattatgg agtggcagta ttgcgccatc atttcgtaac gaccagattt ttctaaaaat
      241 atcaatggtg agggttggca tgtcaccgtg ggtgagaaaa caatgttctg tttgcactgc
      301 gggtactgcg gccttgactg atgttagtaa accctgggca taatctgggt tgtgaataat
      361 agtgatatta ctctggttcg cataacgttc gtgcagttca ttaccacgat agccggtgac
      421 taaaataatt cggctacaaa actgcaacgc atttttgata cttgtatcaa gaattgttcc
      481 ctgttgccag ggtaacatca ttttccattg ccccattctt gatgataatc cggcagcggt
      541 aattatacag tcgatggctg acatagtggt tccattattt ataggggcta ataacgcctg
      601 ataattgcgt tacttcctga tggattaagt tgggggtaat ttatcccaga gaggtcataa
      661 agactcgtga aaagtatagt tgacccatcg gcattattca ttgatttagg tgcacagaaa
      721 cgtcccactg tgatttctgt tgttggtgct ggggggaaaa ccagcttgct tttttggctg
      781 gcagaactgc tccagactcg cggcaggcgt gtgttaatca ctacgacgac acatatgttt
      841 atgccaaagt cacactggcc cgtggttttc tgtcgtgatc ccgccatgct tcctcatgcg
      901 tctcttacat cccccatttc attttgtttt cacagctgga aagcgaacca gggaaaagtg
      961 cagggattta cgccagaagc gattgatgca ctggtgcaac gaccagagtg tgacgtaatt
     1021 ctcattgaag cagatggctc gcgtggaatg ccgttaaaag cgcctgatga gcacgaacct
     1081 tgcataccca aaagcagttg ctgcgtgatt gctgtgatgg gaggacatac tttgggcgcg
     1141 aaagtgagca cagaaaatgt ccatcgctgg tcgcagtttg ctgatattac cgggttaaca
     1201 cctgatgcaa ccttgcaact gagcgatctc gttgcgctgg ttcgccaccc tcagggggcg
     1261 tttaaaaacg taccgcaagg ttgtcggcga gtctggttca ttaaccgttt ttctcaatgt
     1321 gagaatgcga ttgcacaaag cgagctcctt caaccgctgc aacaacatga cgtagaggca
     1381 atctggctgg gcgatataca agaacatcct gcaatcgcgc gcagatttgt gaattagcgg
     1441 cccgggtatt ccgggttact gaaaggatat ccattgaggt tggtatgaat attttcacag
     1501 aggctgcaaa actcgaagag caaaattgtc cgtttgcgat ggcgcaaatt gttgatagcc
     1561 gaggctcgac tccccgccat tctgcacaaa tgttagtgcg cgccgatggt tctatcgtcg
     1621 gtacaattgg tggcggaatg gttgagcgga aggtgattga agagtcgctt caggcattgc
     1681 aggaacgtaa gccgcgatta ttccatggac gtatggctcg taacggtgcg gatgctgtcg
     1741 ggtcagattg tggaggtgca atgtcagtat ttatcagcgt ccatggtatg cgtccacgtc
     1801 tggtgttgat tggcgcgggg catgtcaacc gggcgatagc ccagagtgcg gcgctattag
     1861 gatttgatat tgccgttgct gatatttatc gcgaaagcct caatcctgaa ctattcccac
     1921 catcaaccac gcttctccat gctgagtcgt ttggtgcggc agtggaagca ctggatattc
     1981 gccctgataa ttttgtcctg attgccacga ataatcagga tcgtgaagcc ctcgacaaac
     2041 tcattgaaca gcccattgca tggttggggt tgctggcaag tcgtcgcaag gttcagcttt
     2101 tcctgcgtca attgcgtgag aaaggcgtgg ctgaagaaca tattgcccgt ttacatgcgc
     2161 ccgttggtta caacataggt gcggaaacgc cgcaggagat cgccatcagc gtgctggcag
     2221 aaatattaca ggtgaaaaat aacgcgccgg gtgggctgat gatgaaacct tctcatcctt
     2281 ccggacacca gctggtggtg attcgcggtg cgggggatat cgccagtggt gtggcgctac
     2341 gtctgtatca tgcgggtttt aaagtgatca tgctggaagt ggaaaaaccg acagtgattc
     2401 gttgtaccgt ggcgtttgcc caggccgtgt tcgatggcga aatgacggtc gaaggcgtca
     2461 ctgcccgcct ggcaaccagc tctgcggaag cgatgaaact taccgaacgc ggattcatcc
     2521 ctgtgatggt agatcccgcc tgttcattgc ttgatgaact gaaaccgctt tgcgtggtgg
     2581 acgctattct ggcgaaacag aatttgggaa cgcgggcaga tatggcacca gtaacaatcg
     2641 cgcttgggcc tggctttact gctgggaagg attgtcatgc ggtaattgaa acaaatcgcg
     2701 ggcactggct cggtcaggtg atttactctg gttgtgcgca ggagaatacc ggtgttcctg
     2761 gcaatattat ggggcatacc acccgacggg tgatccgtgc tcctgctgca ggcattatgc
     2821 gatccaacgt gaaattaggc gatctggtga aagagggcga tgtgattgcc tggattggtg
     2881 agcatgaaat taaagcaccg ttgacgggga tggtgcgtgg cttgttgaac gacggactgg
     2941 cagtggtcgg tggttttaaa attggtgata tcgatcctcg tggtgaaacg gctgatttca
     3001 ccagcgtttc tgataaagcc cgggcgattg gcggcggcgt acttgaagcg ttaatgatgt
     3061 tgatgcatca gggcgttaaa gcgacgaaag aagtgctgga agtggcttaa atgaaaatgg
     3121 ccggatggta ttacccaccc ggcctgagac tgatgacaaa cgccaaattg cctgatgcgc
     3181 tacgcttatc aggcctacaa gtgcattgca atctattgaa tttgcgatgt tttgtaggac
     3241 ggataaggcg ctcgcgccgc atccggcatc aacaaagcgc acttacttga caatctttgg
     3301 ccagatggta ttacccaccc ggcctttaaa ttactgacaa atcaccgttc cggttttgcc
     3361 ttcaatccct tctttcgctt tgctcaatac ggtaatcagt gcttcacgac ctgcgcgaga
     3421 gcgagcaaac gaggcggcag cttccacttt aggcagcata gaacctttcg caaaatgacc
     3481 ttcttcgata aagcgttcag catcacttaa tgacaggcga tcgagccact gttcattctc
     3541 tttaccgaag ttaatggcca ctttttctac cgcagtcaga atgatcagca tatcggcatc
     3601 aatcatttct gctaaacggg cgctggccca gtctttatcg ataaccgcac tggcaccacg
     3661 caggtggtta ccttcacgaa taacagggat accaccaccg ccaacggtaa tcactacctg
     3721 gcttgcttct accaacgctt taaccgtttc tttttcaatg atatcaaccg gttttggcga
     3781 ggcaacaaca cggcgatagc cgcgaccagc atcttctttc agagtgtaac cctgttttgt
     3841 cagttgctct gcttcttgct cggtaaagaa cgaaccgatc ggcttggtcg ggttgaggaa
     3901 tgccgggtcg ttagcatcga cttcgacctg ggtaaccagc gttgctacag gtttattaat
     3961 gccacgagaa agcagttctt ctcttaatgc gttttgtaga tcgtaaccaa tataaccctg
     4021 gcttaaggca acacagacag acatcggcag cattggcgag tgtgcttcag tttttgcggc
     4081 agcttcaaag gcctggttaa tcatgcccac ctgtggcccg ttaccgtgag tgacgatgac
     4141 ctcatgtccc tgggcaatta aatcaacaat tgcctgagaa gtaattttta ccgctttcat
     4201 ttgtccggcc aggtcatcgc ccagcgcatt cccgcccagg gcgagaacaa ttttcttact
     4261 catttttcct cactcatcat tcttgattgt gtatagatcc ctggcagtgg ttagagcacg
     4321 ggagggacaa acggtttgcg tcgcaggaaa cgacctcgcc cggctttgcc tgtaaaggtg
     4381 ccgtcacaga aaatcgtttc accacgggat aaggttctga caatcgcgcc ctgacaggta
     4441 aaaccctccc atggcgagta gtcggcgttg tcgtggagat ggcgatgctg aatttgttgg
     4501 ctctgacgtg ggtcgataat caccacgtcg ccatcggaac cgggcgctaa taatcctttt
     4561 tgcggccaca ggccaaacag cctggcgggc atcgcgctgg ttaattcaac aaaacgttcc
     4621 ggtgttatac gtcccgtcat cacgccactg gaaaacagta actgcatgcg gttttccaca
     4681 ccgggtaagc catttgggca gcgactgaaa tcgcctttag aaatttgcag gcgttgagcc
     4741 attgagaacg tgcagtgatc ggtcgccacc acgtcaatcg caccatcgct gatgccacac
     4801 cacagtttgt cctgctcgcg tacgttacgc agcggtgggc taagaatgaa cttcatgcca
     4861 tcttctgtat cgtagctgcg ttcgtccaac aggagatatt gtgggcaggt ttcaacccag
     4921 actggctggt gattcgcacg ggcaagacgc agataatcca gacctaagcc gttagacagg
     4981 tgcacgatat agagcggggc gttaccggca atttgtgcca ggttaatcat gcgggcgatg
     5041 gcttccgctt cgcattccag agggcgactc aaggcatgat agcgcggtgc gattaaccct
     5101 gcggcgataa attccgcccg cttgctggcg atagcagcat cattttccgg gtgcacggtg
     5161 gtcagcgcgc cggattcatg cagacggcgt aatgcctgca aaacctcgtc atcgttgagt
     5221 ttgtattgat aggttaaata gagtttaaaa ctgctcagtc cttcctcgac catcatcggg
     5281 atttcgtcga ggattgcgtg attaatgtgc tggatcacac cgtgaaagct gtaatcgatg
     5341 accgctttat gggcggcata accacgataa acctccagtt gatggcgtaa ccgacagccg
     5401 tttgggccaa atcccatatg gtcaataatg gttgttgtac cgccacacgc agctgcgcgg
     5461 gtaccggtaa aaaaatcatc acaactgcgc gcgatgccga catcaatatt gaaatgcgta
     5521 tggacatcca cgccgccagg gaaaacgtaa cagccagtgg catcaatttc ttcatacggg
     5581 agctgcggcg aaatattgtt gcccaactgg cgaacaatcc cgctttcaat cagcaaatcc
     5641 tgtttggctt gtccatctgc gttaacgaca gtgccgtttt tgatcaatac gcgcatagca
     5701 aactccaaaa accctccggc gaaccggagg ggaataagtg aagagtaatt attcggttgc
     5761 cagccagctt aacgggattg cagcgtacat cgcggcacag gtcaccaggt gagatttcca
     5821 ggttttttcg ttaggtgcat gcgcttcagg ttctttaccc gggccaaaac cgatgaccgg
     5881 aatgccatga cggcccatga tggatacgcc gttagttgag aaggtccact tatcaacaac
     5941 cggagctttg ccaaacagac cttcgtaggc attcaccagc gttttaacgg tgaaatgatc
     6001 ttcttccact ttccaggtcg ggaagtagca ttcggttggg taaaccaggc cagtccagga
     6061 cggacggtcg tagttgtaca tagaaacgac agcattagct ttttgtacgg ctggcagagc
     6121 gcggatttca tccagcgcgc cttcccaggt ttcgccccag gtcagacggc ggtcaataga
     6181 gacggcgcag ctatctgcta cagcgcaacg gcttggggat gtgaagaaga tttcagaaac
     6241 ggtgagggtg cctttgccga ggaattcgtc ataacccaga cgttgggaga gttcttgtaa
     6301 ttcgccaaga atcggaccca ttttgaaaat ggcgttgtca ccgcgttctg gcgcagaacc
     6361 gtggcagcta acaccctgaa catcaatacg aatttccata cgaccgcgct gaccacggta
     6421 tacctggcag tcggttggtt cggtactgac cacaaattcc gggcgaatgc cggattgttc
     6481 aataatgtac tgccagcaca gaccgtcgca gtcttcttcc tgcacagtac cggtaaccag
     6541 cagggtatat tcatcttcca gaccgaggtc tttaatgatt ttaccggcat aaaccataga
     6601 tgccatgccg ccttcctggt cggaagtccc gcgaccaccg atcagctcat cagtttccat
     6661 gccttcgtac ggatcgaagt cccagttttt gatgttgcca atgccgacgg tatcgatatg
     6721 agcgtccatt gccaccagac gcgggccgtg gccgatataa ccgagaacgt tacccatcgg
     6781 gtcgatttca actttatcga agccgacttt ttccatctct tctttaatac gatgtactac
     6841 gcgtttctcg tcgcagcttt cactgggaat agcaaccatg tcgcgcagga agcgagtcat
     6901 atccgcctgg taatcttttg ctttttcaag aatcagtttg aatggaatat tcttagccat
     6961 tatttttctc cagatttttt actccccgtt ttgcagggag cagttccaat agggtaatta
     7021 aggtgctaca gcgtgtttgc cttcccagac aacttcgcgg tagtgcttca cgtcggtgtc
     7081 gccttcagtg ctgataacca gcactacggc atctttgttc agcgccagtt tttccatcag
     7141 gctttgacgt tgcgggtgat aatgaaccgc cgcgagaacg cccaaaccga cagcgccgga
     7201 ttcaccggag atgatgcgcg ggtcgttgcc gtacggatta cccagcacgc gcatacctaa
     7261 tgcggcaact ctgtcctggc aggagatgaa ttgggtggcg cagttacgta ggatttccca
     7321 gcccagcggg ttaggttcgc cacaggccag gcccgccatg atggtggcca tatcaccgcc
     7381 aacgttgacg atgtcgcctt tgacgccgga gcgataaata cagtcagctt tgtcaggttc
     7441 aacaataatg ctgtgcagat tttgcgggct atagacgtcg accagataac ccagcacacc
     7501 accggccatt gctccgacac cggcttgcag cagaacgtgc gtcggggtta cgcccatttc
     7561 acgcatttgc tcgacggctt catctgccag ggttgcgtag ccttgcatga tccaggttgg
     7621 gattttggtg taaccttccc atgccgtgtc ctgtaccact tcccagccgt gctgctgcgc
     7681 gtgttgcatg gtcaggcgaa cggtatcgtc atagttcata tccgtgacga tgcactcggc
     7741 accgaggttc agaatggcgt caacgcgttc ctgagcagaa cctttcggca tgtaaatcac
     7801 cgcattctgt ccgagttgct gcgctgccca cgccacaccg cgcccgtggt tgccgtcggt
     7861 ggtggtcgcg aaagtcattt tttcgccgat ggcatttttc aggtgctcaa atgacagcgt
     7921 ttcgatatca agatgatatt tttcacacaa taattgagcg atagcgtacg caccgccgag
     7981 cattttgaag gcgttcagac cgaatcgttt tgattcgtct ttgacgagaa ttttcttcac
     8041 accaaaaagg tttgcgagat cgtccagcgc acaaagcggt gttgggcgat aaccagctat
     8101 tttctggtgg aactggcgcg ccagtttggc ctggctttgc gaaaagagcg gtgatgtttc
     8161 gccgttgaaa aatttgttat cggcgatatc aatcttcaat gagaaaacgg acatatccta
     8221 tccttttggc ctctggaaat agagggccga ccagctgatc ggcccgaggg gttatttcac
     8281 gcgttcttgc gcttctttca gcagttgctc gagcagtgca cctggtttgg cgtatttacg
     8341 ggacaggatc atcgcggcga tgatataagg cttccagctg gcttctttgt aggtagcgat
     8401 acggtatttt tcgaatacgc cttcagtcac ttcaccttct ttgcaggata cgccgctgat
     8461 atcagctggc aggcagtgca tgtacagggc ttcgccatca cgggtcagtt ccatcatctc
     8521 ttcagtacaa tgccagtctt tgtgttgcgc gttctgtgcc agacactgtt tttccagtgc
     8581 ttttaagcct tcgtgatcgt tcgcacgcag caattcagta cgctcttcca tcactttgta
     8641 aggtgcccat gacttcggat aaacgatgtc tgcgtctttg aaggcttctt ccatgctggt
     8701 gacctgacgg aagctaccac cggaggcttt agcattgttt ttcgccactt caaccacatc
     8761 cgggatcagg tcgtagcctt ccggatgggc cagggtgaca tccataccga agcgagtcat
     8821 cagaccgatg atgccttgtg gtacagagag cggtttgcca tagcttggag agtaggccca
     8881 ggtcatggcg atttttttac ctttcaggtt ttccagtgaa ccaaagtgtt cacgtaacca
     8941 cgcgaggtca gccattgact gagtcgggtg gtcaatatcg cattgcaggt tcactaaagc
     9001 cggacgctgt ggcagtacac cctgcttgta accgtcatca agtgcagcgc caacttcacg
     9061 catataggcg ttgcctgcgc ccagatacat atcgtcgcga ataccaatag cgtcggcgca
     9121 gaaggagatc atattggcgg tttcacgcac ggtttcgccg tgagcgattt gtgatttgcc
     9181 ttcatcgaga tcttgctgtg caaggccgag caggttaagc gcggaagcat aagagaagcg
     9241 ggtacgagtg gagttgtcgc ggaatacgga aatacctaat ccactattaa agactttggt
     9301 tgagatgttt tcagcacgca gtgcttttaa tgctgcggca acgtccagta cttgtttcag
     9361 ttcatctggc gtctgttccc acgttaacaa aaaatctttc tcgtgaaggt gagaggtcag
     9421 cgaattgata tccttaatca gctcattaac agttttcatc ataaagtccc ggtagtggaa
     9481 tgacaggcaa agtggtttat ttcactgtga cttgccaaag tgattggtgt tttcacagct
     9541 atacctggga ctaacaataa gtgtgccaga attatccacg gggctgcaaa agactaattt
     9601 gacggataat gtgtggggtt gatcacaaat tgatgccagc ttccggttgt ttgtaaaata
     9661 cgaaaacgtt tggctttttt tattgcgtta tttatttgct gctaagtatc agaaataaga
     9721 taaaggggca ggctatccct cgtcagtttg ataaac
//
LOCUS       DAZUCE010000192         9591 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZUCE010000192 DAZUCE010000000
VERSION     DAZUCE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.192"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(138..599)
                     /locus_tag="ACHY03_003943"
     CDS             complement(138..599)
                     /locus_tag="ACHY03_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFS3907121.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQDYGRWSEKIMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQEEARQQKRGLWADSAPVPPWVWRSRK"
     gene            complement(1341..1544)
                     /locus_tag="ACHY03_003944"
     CDS             complement(1341..1544)
                     /locus_tag="ACHY03_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907122.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            complement(1698..2255)
                     /locus_tag="ACHY03_003945"
     CDS             complement(1698..2255)
                     /locus_tag="ACHY03_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFS3907123.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            complement(2358..3218)
                     /locus_tag="ACHY03_003946"
     CDS             complement(2358..3218)
                     /locus_tag="ACHY03_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3907124.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(3277..4023)
                     /gene="traX"
                     /locus_tag="ACHY03_003947"
     CDS             complement(3277..4023)
                     /gene="traX"
                     /locus_tag="ACHY03_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFS3907125.1"
                     /translation="MTTDNTNTTRNDSLAARTGTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNLSRHANIRQSAINRLWGWAVIAQF
                     AYYVAGFPWYEGNILFAFAVAAQVLTWCETRSLWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALLACLLAVIPALNLASSDVAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWPGDFFPVFYACHLTVLGVLAV"
     gene            complement(4043..9313)
                     /gene="traI"
                     /locus_tag="ACHY03_003948"
     CDS             complement(4043..9313)
                     /gene="traI"
                     /locus_tag="ACHY03_003948"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFS3907126.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMLTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVSRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKISLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKIPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVMAVSGDSVTLSDGQ
                     QTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVLEPGADREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVWIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAEEKPDQPDGKTEQAVRAIAGQEQDRASSSERETALPESVLRESQRVQDAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            complement(9313..>9591)
                     /locus_tag="ACHY03_003949"
     CDS             complement(9313..>9591)
                     /locus_tag="ACHY03_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFS3907127.1"
                     /translation="VSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISES
                     GEVVDMTAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         2014 a         3008 c         2264 g         2305 t
ORIGIN      
        1 gatgtgatta attctgatac tgcctgatag ttcatttgtt cacctttcat taacagagaa
       61 ataagttaat aatgtaatat gcttcatgct tttaaatatc gcatttcaat gagaactcaa
      121 tagccacaga aatatcatta tttccgtgag cgccataccc agggcggaac aggagcgctg
      181 tctgcccaca gaccacgttt ctgctgtcgt gcttcttcct gcaggacggg aagaacagga
      241 tcggtgttgt actgctcata aacccatgca gcgcctgcac ggaccataaa ctgattaaca
      301 ttcataccgt ctggtgcata aacctgcccg agaatacgac cataacgatc tcgctgaaaa
      361 tatgtgacag taaccgtttt tcccgcaacc agagatttca ttattttttc agaccaccgg
      421 ccatagtcct gtttcttttc aggtgcatca atattaacaa gacggacacg caccggatgc
      481 tgagataaca tgacgtcaat ggtatcgcca tccagaacac gcacaacacg tccatgaata
      541 tcagcagaaa atacctgaca tgaaaaaaca aataatacca ctggtatata tttcttcatt
      601 ttactctccg tcactgatta cttttcgtga ttgcatgaat tccatgccgg gatgaataac
      661 ggctcacgca tcacgccaaa aataaaccgt tgaattgtga tcaattcgac aaaataagat
      721 gaggtggctt aatacacata tggctatgtg ttactaaaaa cgctggtcac cccgcctgac
      781 cagacacata agaaagaaag tctcatgcat tattcagcct tctgatacct ggagtaacgg
      841 aggctgcttt ttctgtcacg tatttcccgt gacggcgtta ccggtttccg ggggtaacat
      901 gtgacggaga ttatattttt aatctccgtg tattcatcat acctgctctt actgatagga
      961 taactgtgtt atatcgcagg ctaccggatg tggcgctgaa tataacggtc atagataagt
     1021 catacatgcc ctgctcaggg gattagcgat aagacggacc gggagagtca tctcccggtt
     1081 tttctttatc ctgccgcgaa tacagcccgg ctgtctgtca gattcaccgg acataaccgt
     1141 actgttatgc atgccactgt caccttctgc ttcttgaaat tcatcacatt ttttatccct
     1201 gttggcttac ccgggatgtg aaatatgtta attctgtgat gtccgtttga aagacagggc
     1261 aactccttct tacaccacta taataataac gatctgccat ataagtctga tcccccggcg
     1321 ctccgcaacg ggggttattt ttatcggcac ttacaccggc atgcagatgc tctcttcagg
     1381 gcaaaatccc gccatgcttc acagggtgcc agccgggccg cattctgcca ggcctcagcg
     1441 gcttccctga aaaaaccacg aatctcctga cggacggcat gacgccccac gcgctgatag
     1501 cgataaatac cgctgtgctc agataatcca ctggatacag ccatttttta tcccccctct
     1561 tgacccggct cccggattat gtcagaaaca ttatgaaaca tccctgaacg gttgggtgtt
     1621 tttctccatc aaaactctgg atgtctgttt tcccgatttt ttcgctgttg ccctctgtca
     1681 tacagagggc gtttttatta ttctgccagg atcgcccgca gctcatcctt gatccggttc
     1741 tgtctgcgca ccttttccag gcgcgcctgc gcatactgtt cttcttcctg tgtgatatgt
     1801 tctgtcacat agccttccgt atcatagcgg caggcgccgg ctttcattgc acacagataa
     1861 ctttccgaac gggtgatggc tttcagcgcc cggctgagtt ttttatgaga gagcgggata
     1921 ttccgctgaa acacctcatc cagtaaaacc tcccggatac cacaggcaag aagccggggc
     1981 gtgttaccgt caaacaatcc cggccaccag ggtttcagag tgttcaccgc ctcatccagc
     2041 gagggcagcg tcagataaat ggacagcgcc tgtctggcct gtgctttctt cgccgccagc
     2101 tctgcttccc gggcagcctt ctccgccagt ttctgctttt tcaccttcca ttttggtggc
     2161 gtggtgacat tgatgatggt tttccggctc cgggtcggtg tttccccttc cgttttccgt
     2221 ttcagtgtca gtaccggtcg tttctgctct gtcatgattg ccctgacctt tgtgaaaata
     2281 tgtcccccag gtcgttgaaa ctactcacta tgatattaag ctgacctcaa cgacctctga
     2341 catattaata ccgacaatta aagatgtttt ttaaaccatt gcgtctgtac tgccgccaca
     2401 cgttcaaaaa acgcgccttc ataaatatca taatggcatg cgtcagcttc ctcgtataat
     2461 tccttagtac cagaagcaac agcatcatat aaggctcttc cctgttctgg tggattaaca
     2521 ctatcctggc cggcaataac aaccaaaact gggcactgta ctttggctgc gctctctgcc
     2581 ggtttatatt gcaacgtctc cataaccgta aggaaaggta tttttatatc catttcagga
     2641 tactgacctt ttaccttctc gaagaatacc tttgattcat tatcactcag tacccgggta
     2701 acccccacaa acatctcttt cccggtattt ttcttctttt ctgccatttt attcagcgtg
     2761 gacagaaaag aagctttctc cagttcattc atttctccgg taaccagaac ctcaccgtca
     2821 gcaaaagcca gctggctgac aatacttttc actcttttat cctgcgcagc tgcattgaat
     2881 acatgacacc cacccagcga tgttccccat aatcctatac gctgattatc aatacatacc
     2941 tgcttttctg cccaattgat aactgaaata atatcttcgg tctgcattgc cggaaccaga
     3001 cgacctcgtt ccccttcact ctcaccgaat ccacgataat caaaagtaat cgttgcaaac
     3061 ccggcttctg taaacgcatt cgcaaaggaa ggcagcagaa cattccggat accgcaaaat
     3121 ccatggcata agagaatcaa aggatgtttt atatttcctt ccgggacacg aaatgtcaga
     3181 gcaatacctt cagggagttt gtgatccgta attttcataa atatcctgtg ttatgttgtt
     3241 tattaataag cagaatcagc gccagcatca taccagtcac acagccagaa cgcccagcac
     3301 agtaagatga caggcgtaga ataccgggaa aaaatctccg ggccagaaac ggggtaatga
     3361 tttccctgta cacgacacca gaccaacggt cagcaccgtc attacaagac cagctacggc
     3421 cgccacatca ctggaggcaa ggttaagtgc cggaataacg gccagcaggc atgcaagcag
     3481 cgccagacgt tccgctctgt cttccgcccg gtacagccgg tgactgactg ccagcatcag
     3541 cagcccggca atgccgtaac tggtgccgga caaaggcccc cacagtgcca tcaggagtat
     3601 ggccgcggca gtacgccaca ggctgcgcgt ctcacaccac gtcagcacct gtgccgccac
     3661 tgcaaaggca aacaggatat tcccctcata ccagggaaag ccggcaacgt aatacgcgaa
     3721 ctgggcaatc acagcccatc cccacagccg gttaatagcc gactgccgga tattcgcatg
     3781 acgggacaga ttcagtcccc agaccagcgc aaacagcgga aacgcgcccc gcccggccag
     3841 aaacatccac gtctgctcca gatgcagaat gcggtttgca tggtccagca ccatcagcac
     3901 cagtgccacc gttttgatga tatcccgctg tccgggtgac cagaccagca acgactgcag
     3961 ccaggtaccg gtccgagcag ccagtgaatc gttacgtgtc gtgttcgtgt tatccgttgt
     4021 catcagcgtt tgtcttcctg tatcagtctc cgcccagggt tttctctttc tgcaggtcac
     4081 ggaccatatc ccgctccatc tgctgaagtc gctcctgcag cagattttcc cgggcaacct
     4141 cccggacagc gtcctgcacc cgttgagact cacgcagcac actttccggc agcgcagttt
     4201 cccgttctga tgacgaagcc ctgtcctgct cctgcccggc aatagccctg acagcctgct
     4261 ctgttttacc atccggctgg tcaggttttt cctctgccat ttttctgaca atctcatccg
     4321 cacggcgttc cgtttcacga cggatggcct cttcagcctg ccgctgtgcc agcacctctg
     4381 ccgtgaccgg ctcaccgttt ccggcccccg gctggacgct gttgtccggg atatcccccc
     4441 acacgcggcc accggtgatg gcaccgggat tccacggacg accttcaccg gcgattctca
     4501 ccaccacacc gctgtcgggg ttatcacggg caatccagac accatcctgc atattatcag
     4561 ccagcaggct ctcgccgtta cggctgccct gcagggccac aaactgcgca tccccgctgc
     4621 ctttcacccg gccttcacca ctgaatcccc gcagcccgtt tccgtcatcc gtggtcagtg
     4681 ggttcagcca gataccggcg gacttgccgt tacggtcaaa agccggcagg gccacatacg
     4741 gctgcggata tttgcgcccc ggtgcaataa accgtgccgg actgtcgccc ccggccagtc
     4801 ccgcctggcg gagaacagca cggccagctg ccacatcccg cagctcccgt gccgtactga
     4861 acaaccgctc tgcattcatg acctcccggt cagcccccgg ctccagcaca tcatgggccg
     4921 ttcctttctg tacggctttg ctgatggcat ccgtccagcc ctgacggtta tcggtgtaca
     4981 cctgcacatg ctgcttcata cgcgacaggg ccacataggc tgactcaaag ccggccatct
     5041 gtttccggtt accttccgtg ccttcaagcg cgatggcaaa ggtttcactc gccccctgcg
     5101 caccgtgagc ggtgatggca taggccaggt caatatgttg ctctgcctgc tcctggccgg
     5161 ggcgaatcac ccgggtctgc tgtccgtcag aaagcgtgac actgtcaccg gaaaccgcca
     5221 tcaccgtcca gacgctgttg gccacatagc cgcgctcccg gtcactcttc gtgaagcgca
     5281 tccggtcacc ggtccccacc cggatggtgt ccggggtgta cagtgtgaca ccttcagcca
     5341 ccgcctcccg gggcgaaatc agccgcgtgt taccttccgc atcctccagg gttatcagcc
     5401 cgtcatcctt actgataccg gcaatccggt gatacacatt atccaccagg gcaagtgcgt
     5461 cccgatgtgt ctcccaggtg gagagacgac gcagctctcc gtcacgtata ttcgctgtgt
     5521 tcaggacagg caccatgacc tgctctttcc ccagctcacc ggccttttcc cgtacatcat
     5581 gaatcatgct gttcagtacg cgccggtcct cattcaggtg cgtgacaatc agcgtctgct
     5641 cccgtgcttc cggtgttctg ccggtatagt cgcggacaat ggcttcatac agtgtcatgg
     5701 ggacatccgg aaaagcctcg cctttcagca tcgccttctg ctgcgcttct gccagtttcg
     5761 cttcctggct gtgactgaac tccgtcacgg agtgctccgg tgcccatgcg ccctcctgac
     5821 gtggcacctg agacggtttc acactctcaa gcccggacag tgccctttcc acatcccggt
     5881 taatcaggct gtataccgcc tcccgcagtt ccggcgtctg acgcacaatc tccttcatga
     5941 tgaccacatc ggcagcactg cgcgtctgct ggagacggaa aggctgaccg ggcgcgatag
     6001 cctgcagctg gtccgtgtca ccgctggcca cggcacgacc accaccggcc gcaatcaggg
     6061 cgtatgcccg tgccatgtcg gtattgccca ccatagagct ctcatcgagc aggaacagcg
     6121 tgttgctgaa atccggcgtt tctccgctgc gctgctgcag ctgcgtgtca tgcagaaagg
     6181 atgccagtgt ctgtgcatcc acgccggcgc tgcgcatctc accgaccgca cggtgcgtgg
     6241 ggcccagccc cacgacacgg ggacgctcac tctccggcag catcttcacg gctgacatca
     6301 ccgcccggaa ctgtgtggtc ttacccacac cggcataacc ctgcaccacc gtgaaacggt
     6361 cggacgtttc cagtatcatg cgggtggcgg cacgctgccc cgacgtcagt gtctccatga
     6421 gttcgccggg taccctctcc atcagcggcg tgaccgcctc cttaccttcg agaatatggc
     6481 gaagaatgct cttttctgcc tcatacgacg cacgggaaac cagcaggcct gtgccatagc
     6541 cttttgccac atccacatac agcaaatcac cgcgttttat ctgcgcattg atttcccctc
     6601 ccagttcagt aaaactggtt ccttctgcag caaacgactt cgcctctgtc agcaggtcca
     6661 ccatgctgaa ggccaggttt ttactttcca gcacaggaag ggccagatga atggcctgct
     6721 gtgccggcgt gtgaagcccg gctttctgca gactgatagc ggtttccagc aatgtctcac
     6781 cggcacgcgc ctttatctgc tcagaaacca ccgtaaagga gggatggcgg gcaagttttt
     6841 ccgcagtacg ggtttcatcc agtgaggaat acagccggac atcacgaccg ctgcgggcca
     6901 gaccgttcag ggtggcgttg tccatcgcca tctgtgtgac ggaggcaaaa accttcgcac
     6961 tgtcgctgac ggaatgcccg ggcgtttcca cccagccgtt ctccagcttc agtgccgtga
     7021 acggtgaatc gctcacaggc agggtggccg gttcagcacg tcccggcaca acaaccgtca
     7081 tcgcatcttc actgacggat gccacctgca ggcggtcacc gccggagacg cggagtccgg
     7141 gaattttccc tgtcaccctc agacgctcac cgtctgccac cggtattttt tccggccgga
     7201 acagcgacca gctgctgtcc agggaggaaa tacgcaccac ctgcgtttcg ccctgtgcat
     7261 cccgcagggt caggctgtga ctctgcgctg tcacccggtc gataacatag cggtcatgac
     7321 tgcgtgtctc cgggttccac tgctccatca ccatccccgg acggtacata tcccgcagat
     7381 aacggctccg gctgtccagc cagaccggtg acagtgcagt catggtcacc tcagggtgtc
     7441 cgagcacgcc ctgcgttttc agctcactgc gaatggcctg tgtcagtatg gcctgttccc
     7501 gtaccccgct gacctgtgcc acgctctctt ctccggcttt cacgctggcc gcaaaatctc
     7561 cggccagccg ggcatagcgg acattacggt ccggttcact gatgatggtg gccggtcgct
     7621 gttctccccc ctgccagcga tatgtgttca ccccggcatc cttcatggcc atcagtgcac
     7681 tgcctgtacc ggttcgctgc ccgctgtcgg ttatcaggac ctgtacgtta tgacgtgcgg
     7741 caccgtccag cagggttaac gtctctttca gggagatttt ttcgccctgg tcaacgataa
     7801 cggtactgcc cggtgtgaag gccatgcctt ccagcaactg acgacgtccg gttatcagct
     7861 caccggacaa ccgttcatcc tgcttcaggt tcatctgcga gcgacggtca gcagcgataa
     7921 tctgcacctc ccgcccctgc tcccgggcca tcatgaccag ttcagccacc cgctcacgct
     7981 gcccggctgc accgccctgc ccggacacaa tggccagcga cggacggtcc tgtgccagaa
     8041 cactgaccgc atcgctgtaa ccagccgtcc gggagacact tttctccgga tgtacggtca
     8101 cccggttctg tttcatgatg tcacgactga gtgcccggac ggacagttca tcgagcacat
     8161 gaattcctga tgtgaacagc cccttctcac ggtcgagggg gataagctgc tcacgactga
     8221 tggcctcatc gataccggcg cgtgcccgtt caatcacacc attttccggc ggcagtatac
     8281 cgaccgttct ggccagcacg tccgtgtacg taaactgcac ttttcgttca cttaatccgg
     8341 caatcgcctg tgtcaccgcc tgctgcacat ccggcccgtc ctgtgaagcg ggtccgggtg
     8401 tctgcgtgcg ggtctccgca cgctgatccg ccgcgtcacg atatgcccgg atgtcgaacc
     8461 cggtttcctt cagcgtcagc atccattcag ccattctgac ttcaggatcg acgtgctgtt
     8521 tggattttcg cgtatccagt gccgccacat cccgggattt cagcgaggca tcctccccga
     8581 cggcctcccg gatagcctgt gagcgtccgg aaaaggcctc caccggtacg cccggcatct
     8641 cccacatacc gtgcttaccc accacttcgg tttcatagcc cagcgcctca acctgctctt
     8701 tcagtttttc ccggtagagc ctgccaaagg caatctgatt agcgtacaca ttctcaatga
     8761 accccgtttt ccccactttg tcactgctca gtgtcttcca ctcaccgtta tgctgcgtga
     8821 cattagccac caccgcatgc gtgtgtaact gtggttcctg atcgcgactg gtgtcgtggt
     8881 taaacagtgc catcaccaga ttaccggtca gtaccgtttc tgactgtccg tccgtcatca
     8941 cccgtgtgga ggccagcgcc tccacctgac ggacagcaaa gtccacggcc tggttgtgtg
     9001 catcaatcag gcgcttatcg ccacctaaca tggccatcat ggagacactt ttgggggcgg
     9061 agaaggtcag gtcgtagccg ggacgatgcc tgttactgcc atcctgcatg cggcttagat
     9121 ccgctccgtc cggcagcctt ccctccagaa gacgggtaaa aacatcctta tcgacactgc
     9181 cctgcagtcc cagctgttca gcccccctgc cggcccagcg ttctcccatg ctgcccagta
     9241 catagtaatt atccttgtcg gtataatagt taccggcact tccggccgat ctgacctgcg
     9301 cgatactcat catcagaaat catctcccgg ctcaacatcc tccccacgct cccggtgcac
     9361 attaatgttc acctcttcac gacgctgcat ctgctgctgg atgtccggat gattttcctg
     9421 ttgccatgcc tcataagcgg tcatatccac cacttcaccg gattcactga tcccgggtgg
     9481 cagttgctgt tccatctctt cttccggttt cattttcagc tcctgctcga tcccccctgc
     9541 cggaacattc acacctgaat ctgacttctt atcgttgatg gcaggagaca c
//
LOCUS       DAZUCE010000193         9547 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZUCE010000193 DAZUCE010000000
VERSION     DAZUCE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.193"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(48..260)
                     /gene="cspA"
                     /locus_tag="ACHY03_003950"
     CDS             complement(48..260)
                     /gene="cspA"
                     /locus_tag="ACHY03_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFS3907128.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(541..831)
                     /locus_tag="ACHY03_003951"
     CDS             complement(541..831)
                     /locus_tag="ACHY03_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFS3907129.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1265..1975
                     /locus_tag="ACHY03_003952"
     CDS             1265..1975
                     /locus_tag="ACHY03_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFS3907130.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2025..2999)
                     /gene="ghrB"
                     /locus_tag="ACHY03_003953"
     CDS             complement(2025..2999)
                     /gene="ghrB"
                     /locus_tag="ACHY03_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFS3907131.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVDQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3103..3762)
                     /locus_tag="ACHY03_003954"
     CDS             complement(3103..3762)
                     /locus_tag="ACHY03_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFS3907132.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCATNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3915..6248
                     /gene="bisC"
                     /locus_tag="ACHY03_003955"
     CDS             3915..6248
                     /gene="bisC"
                     /locus_tag="ACHY03_003955"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFS3907133.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVNAEKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLVP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTVLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6217..6657)
                     /locus_tag="ACHY03_003956"
     CDS             complement(6217..6657)
                     /locus_tag="ACHY03_003956"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFS3907134.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRECIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6654..7217)
                     /gene="tag"
                     /locus_tag="ACHY03_003957"
     CDS             complement(6654..7217)
                     /gene="tag"
                     /locus_tag="ACHY03_003957"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFS3907135.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7375..8073
                     /locus_tag="ACHY03_003958"
     CDS             7375..8073
                     /locus_tag="ACHY03_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFS3907136.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8302..9504
                     /locus_tag="ACHY03_003959"
     CDS             8302..9504
                     /locus_tag="ACHY03_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3907137.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2236 a         2348 c         2491 g         2472 t
ORIGIN      
        1 ccccgccaaa tggcagggat cttagattct gtgcttttaa gcagagatta caggctggtt
       61 acgttaccag ctgccgggcc tttagcgccg ctttcgatgg tgaaggacac tttctgacct
      121 tcgtccagag atttgtaacc atcgttctgg atagcagaga agtgtacgaa cacgtcttta
      181 gagccatcgt caggagtgat gaagccgaag cctttgtcag cgttgaacca ttttacgata
      241 ccagtcattt taccggacat agtgtattac ctttaataat taagtgtgcc tttcggcgat
      301 atggcgtgct ttacagattt tgaagcgtta aaggaatgtg cactacgagg ggtatcaacg
      361 ataactcttg aagggacttg ccttactaca ctgctttaat ggtctgtacg tcaaaccgtt
      421 gatgtgcatt aagccacgca ttggcgggtg atgcaacaat tatttttcat atttatgatt
      481 aatcggttgg aaaacggtcc tgtcatcagg accgtaaaca gcaataaagt ggataaaagt
      541 ctattccatc aactgcttac ttaatgccgg gttggcttga atcaaacgca tcaattttag
      601 ttcggcactt gaaggcttca cgcgtctgga ttcccattcc tttaccatgg cgactgatac
      661 gcccaaaacc cgggcgaaat catcgatttt taatcctgtc ccttttcgta actgctcaat
      721 ttcggtacag gacgttgttc tgtgcgtcag ggtaattttc tgcgtttcat ctttaaaaac
      781 aatctgttcc aggctgctca acagctcatg cattggatct ttatattcca ttgaaaactc
      841 ctcaaatcac actgcgggat cgtgaattgc atcgaagctc attaagaata gtcgggaaaa
      901 gcagacacag cggggtgaag tcagggcatg ctctggctat gattcgattc agccagtcat
      961 ttagcgtgac ttatagccac ctaacgatct gataaatgcc gatatatctc ttaccactcc
     1021 tggtttgtaa agatttcgaa aatctaacct tctgcgaagg cgtaaatctc tggctacaaa
     1081 aaaggcaacg gcaatatcat tttttaacaa tgaaacaaca aattaacctt gtttcatttt
     1141 ctccatgatt ttaccaggaa gattacgtag aaagggtatc ctgcaagcct gtcctggact
     1201 atccttgtca cgtcagacac gcgtgtgtcg ttgtgcgctt tttttgggtg aaaggagtaa
     1261 gaaaatggcg acaggaaagt cctgctctcg ctggtttgcg cctcttgcgg cgttattaat
     1321 ggtagttagc ctgagtgggt gttttgataa agaaggcgat cagcgtaaag cgtttatcga
     1381 cttcctgcag aatacagtga tgcgtagcgg tgaacgtcta ccaaccctga ctgccgatca
     1441 gaaaaaacag tttggtcctt ttgtctctga ttacgcgatt ctgtatggtt attctcagca
     1501 ggtgaatcag gcgatggatt ccggtctgcg cccggttgta gacagcgtta acgcaattcg
     1561 cgtaccgcag gattacgtta cgcaaagcgg tccgctgcgt gaaatgaatg gttcgctggg
     1621 tgtgctggcg caacaactgc aaaatgcgaa gttgcaggcc gatgctgcgc actctgcgct
     1681 aaaacagagt gatgacctga aaccggtctt tgatcaggca ttcaccaaag tggtaacgac
     1741 gccagctgat gcattgcaac cgttgattcc ggcggcacaa actttcacgc aacaactggt
     1801 gatggttgga gactacattg ctcagcaagg tactcaggtg agctttgtag caaatggcat
     1861 ccagttcccg acttcacagc aggcaagtga atataacaaa ctgattgcgc cattaccggc
     1921 acagcatcag gcgtttaatc aggcctggac tacagcagtt actgcaaccc aataaagagt
     1981 aaaagcccga gcgattatcg ggctttactc cacgcagtcg cggcttagtc cgcgacgtgc
     2041 ggattcacac agttcttctc aacctttcct tgtaacgcat caatcaaatt atccacggca
     2101 caggcggcca tgccataacg cgtctcatgg gtggcagatc caatatgcgg tactgcgacg
     2161 acgttggcca ttgagagcaa cggcgaatct acggacagtg gctcttgttc gaagacatcc
     2221 agcccggcgg cgtgaatttc ccctttctgc aatgctgcga tcagtgcatt ttcgtcaacc
     2281 accgggccac gtccggcatt aatgaaaatg gcggaggatt tcattttggc gaattgttct
     2341 gcgccaaaca gatgatgcgt ctcatcagtt aacggcagga tcaggcaaac gaaatctgac
     2401 tcttgtaaca gagtatccaa atcgcagtag cgggcgttga agcgttcttc tgcttcttta
     2461 tggtggcggc gcgcgttata gaggatgggc atgttgaagc caaagtgcgc acgttgtgcc
     2521 agcgccatgc cgatccgtcc catcccgaca atgcccagtg ttttatggtg aacgtcagtg
     2581 ccgtaccagt ccgggcctat gctcgcggtc cattcgcctg cttttacccg ttctgctacc
     2641 tccacaaccc gacgagcggt agacaacacc agcgccatca gcgtatcggc gacggtttct
     2701 gttaatacgg ttggcgtgtg catcagcaga atttttcggg cggtaagcgc atcgacatca
     2761 aaattgtcat agccgacgga gatcgttgat gtggcacgca gtttcggcat tttttccagc
     2821 aatgcggcat ctacattctc gtttgaaccc agtaaacctt cagcttctgc aaaaattgct
     2881 gcattttgat cgacggtttg tgggctgagg tttgccacct ggtgaacggt gaaatgctct
     2941 tgcaggcgtt gcagtaaatc atcaggtaag gctttgtaga ggataacgga cggcttcatg
     3001 cttctctcca ttcactgata aggcgatcac cttagcctga aatatgatga aggaaaactg
     3061 aagaatgcca ggtgatttct gcatcacctg gcatgaaagg gattacagcg ggcttaaggt
     3121 aatttctaca cggcggtttt gcgccttacc ttctgcggtg ctgttgctgg cgattgggtt
     3181 agccgggcca aggccctgag tacggatgcg gctggcgtcc acgccctggg tgatcaacgc
     3241 gctggcaacg gaatccgcac gttgctggga gagacgcatg ttcaggtcgt gaccacccgt
     3301 gctgtcggta taaccaatca cgttaaccgc cgttttcgga tactctttca gtaccattgc
     3361 cacgccggtc agggtgttag cgcccgccgg tttcagggtc gcgctgctgc tgtcgaaggt
     3421 cacattgttc ggcatattga ggataatgtt atccccgctg cgggttacgc taacaccagt
     3481 gccgcgcatt ttgtcacgca gcttcgcttc ctgcacatcc atgtaataac ccacgccgcc
     3541 gcccagagct gcgcctgctg ctgcgccaat cagcgcgcct ttaccgcgat ctttcttcga
     3601 agaagagagc gcaccaatac ccgcgcccac gagagagccc agacctgcgc cgatagcaga
     3661 tttacctgct tcgcgttcgc cggtgtaagg gttagttgcg cagccagata ccgccagagc
     3721 gccactcact acggcggcaa taagataaac acgtttcttc attgttaatc cttaataacc
     3781 tttttattct ttgccacggg ttccgtggcg ggagattatg ccgcgtgaac atgaagatta
     3841 ttcctgggaa tactcggaaa tttgtaagta atatttaact gctcaataca tctaaccttt
     3901 caggagtctt cggtttggcc aactcatcct cacgatattc cgttctgact gccgcccatt
     3961 gggggcccat gctggttgaa accgacggcg aaaccgtgtt tagctcgcgt ggcgcgttag
     4021 ccacaggaat ggaaaactcc ttgcagagcg cggttcgcga ccaggttcac agcaatacgc
     4081 gggtacgatt tccaatggtg cgcaaaggct ttctcgcgtc accggaaaac ccgcaaggca
     4141 ttcgtgggca ggatgaattt gttcgcgtga gttgggatga ggcgctggat cttattcacc
     4201 aacaacataa acgcattcgt gaggcttatg gtccggcatc gatttttgct ggttcctacg
     4261 gctggcgttc aaacggcgtg ctgcataagg cctcgacatt attacaacgc tatatggcgc
     4321 tggcaggcgg ttataccggg catctggggg attattcgac cggcgcggca caggcgatca
     4381 tgccgtatgt cgtgggtggt agtgaagttt atcaacagca gaccagttgg ccgctggtgc
     4441 tggaacatag cgatgtcgtg gtgctgtgga gtgctaaccc actcaatacg ctgaaaattg
     4501 cgtggaatgc atccgatgag caggggcttt cttacttttc tgcactgcgt gacagcggga
     4561 aaaagctgat ctgcattgat ccaatgcgat cggaaaccgt cgatttcttt ggcgataaaa
     4621 tggagtgggt ggcaccgcac atgggcactg atgttgcgct gatgctgggg atcgcccata
     4681 cgctggtgga aaatggttgg cacgacgaag cgtttctggc gcgttgcacc accggttatg
     4741 ccgtcttcgc ctcttatttg ctgggcgaga gtgacggaat agcgaaaacc gccgaatggg
     4801 cagcagagat ttgtggtgtt aacgcagaga aaatccgcga gctggcggct attttccacc
     4861 aaaataccac catgctgatg gctggctggg gaatgcagcg ccaacagttt ggtgagcaaa
     4921 aacactggat gatcgtcacg ctggcagcaa tgttggggca aatcggcaca cccggcggcg
     4981 gttttggtct ttcttaccat tttgccaatg gtggtaaccc cacgcggcgc gctgcggtac
     5041 tctcttccat gcagggcagc ttgccgggtg gctgcgatgc ggtagataaa atccctgtcg
     5101 cccgcattgt tgaagcactg gaaaaccccg gtggcgcata tcaacacaac ggtatggacc
     5161 gacatttccc ggatattcgt tttatctggt gggcgggcgg tgccaacttt actcatcatc
     5221 aggataccaa tcgcctgatc cgtgcctggc aaaaaccgga gctggtggtg atctctgaat
     5281 gcttctggac ggcggcggcg aaacacgcgg atatcgttct gcctgcgact acctcttttg
     5341 agcgtaatga tctcaccatg accggtgatt acagtaatca gcatctggtg ccgatgaagc
     5401 aagtggtgcc gccacgctat gaagcgcgta atgattttga tgtttttgcc gagttaagtg
     5461 aacgctggga gaagggcggt tatgcacgtt ttacggaagg aaaaagtgag ctgcaatggc
     5521 tggaaacgtt ttataacgtt gcccgacagc gcggggcaag ccagcaggtt gaattgccgc
     5581 catttgctga gttctggcaa gccaatcagt taattgagat gccggaaaac ccggacagcg
     5641 agcggtttat tcgcttcgct gatttttgcc gcgatccgca ggcgcatcca ttaaaaaccg
     5701 ccagcggtaa gattgaaatc ttctcgcagc gtattgccga ttacgcttat ccggattgcc
     5761 cggggcatcc aatgtggctg gtgccggacg aatggcaggg caatgccgaa ccggaacagt
     5821 tacaggtact ttctgctcat ccggcgcatc gtctgcacag ccagctgaat tacagttctc
     5881 tgcgcgaatt gtacgcggtg gcaaaccgtg agcctgtcac cattcatcct gacgatgccc
     5941 aggcgcgcgg catacaagat ggcgatactg ttcggctgtg gaactcacgc gggcaaattc
     6001 ttgccggagc ggtcattagc gagggaatta aacctggcgt gatttgcatt cacgaagggg
     6061 catggccgga tctggattta accgctgacg gtatttgtaa aaacggcgca gtgaacgtgc
     6121 tgaccaaaga tctccccagc tcgcggctgg ggaatggctg tgcgggtaat acggtgctgg
     6181 catggctaga aaaatacaac ggtccggaac tgacacttac agcgtttgaa ccaccggcca
     6241 gctcataatc caggtgggta gttgggtttc atcctgccat gcgcaatcga caatgtgaaa
     6301 accctgcgcc tggtaaaaat ctatcgccgg ttgatttttt tgataaacct ccagcatcag
     6361 atggggatgg cgctgttgca catactgcat cagcgcctta ccaataccgc gcctgacggc
     6421 cttcggtgcg acaaacatcg ctgccagaaa tcggccttcc ataatgctga caaaaccaag
     6481 aagcttactg tcttcttccc agacccagtt ttgtgcgttg gcaagatagg catcccgcac
     6541 cagcggaatg cactcgcgcc agtaattcgc ttttataaag ggatggcccc aggttgtact
     6601 ttccagccac agttcgagga tcgcggggag ttctgaacgt tgcgcttccc taatcatggt
     6661 ttatttcccg gatagcaaca gcagccaacc acatgatcat tcaccagccc acatgcctgc
     6721 ataaaggagt aacagattgt ggtaccgaca aacttaaaac cacgtttttt cagtgcctta
     6781 gatagggcgt cggaggcggg cgtagatgtg gggatttcgc tcaacgttgt ggcttgtgtc
     6841 acctgtggct gatgatttac aaacgaccag acaaagtcgg caaacggttc gccgttctgt
     6901 tccatttgca ggtacgcccg cgcattacca ataattgcct gaattttccc gcgatggcgg
     6961 ataatcccgg cgtcctgtac cagtctttcg acatcctctt cctgcattgc ggcaaccttc
     7021 accggatcga actgatgaaa gcaggcgcga tagttttcgc gttttttgag gacggtgatc
     7081 cacgataacc cagcctgctg cccttccagg cagatcattt cgaacagttt tttactgtca
     7141 gtttcaggca cgccccactc attatcatgg taggcaatat aaagcgggtc ctgactcacc
     7201 cagccgcaac gttccatact ttccctcgct atgtacccga tcgtaaaaat ttcagccgct
     7261 tttcttgacg catcggtgaa aaagtcaata tttaatacag ggctactatg cccgatatcc
     7321 ttcatacaat gacaataata ttgccgaatt cggctcttcc ctggagtcgt gttaatgatc
     7381 ataaaaaaaa gcggtggtcg ctggcagcta agcctgctgg cgagtgtggt aatcagtgcc
     7441 ttttttctca acacagctta cgcctggcaa caagaatata tcgttgatac gcaacccggg
     7501 cattccacag agcgttacac ctgggatagt gatcatcaac ctgattacaa cgatattttg
     7561 tcgcaacgta ttcaaagtag ccaaagggcg ctgggactgg aagtcaatct ggcggaagaa
     7621 acccctgtgg atgtgaccag cagtatgagt atgggctgga attttccttt atatgaacag
     7681 gttacaactg gcccggtcgc ggcattacat tacgatggca caaccacctc gatgtataac
     7741 gagtttggcg acagtactac cactctgacc gatccgttat ggcatgccag cgtgagtacc
     7801 ttaggctggc gtgttgactc ccggcttggc gatctccgac cctgggcgca aatcagctat
     7861 aaccagcaat ttggcgagaa tatctggaag gcgcaatcag gcctgagccg gatgacggcg
     7921 acaaaccaga acggcaactg gctggatgtc accgtaggcg ctgatatgtt gctcaatcaa
     7981 aatattgccg cctatgccgc gctatctcag gcagaaaata ccactaacaa tagcgattat
     8041 ctgtatacga tgggggttag tgccagattt taacgtaaca atcacaatta aaaccattga
     8101 ataacaatag ttgtggcgat agtgggtgct aacttaccaa ataataaatt tggtgaataa
     8161 ttgtcgcgtc attcattcct gaactaaggc atttcattcc gttctgatgg catttcatgc
     8221 cgtttttccc caggcataaa gtgcacttcg ttatggttgt cggcagagat ttttcctttt
     8281 tattactgca ggaatactgc catgacacct tcaaattatc agcgtacccg ctggttgaca
     8341 ctcatcggta ctatcattac ccagtttgcg ctggggtcgg tttatacctg gagcctgttt
     8401 aacggcgcgc tttccgccaa gctggatgcg ccggtaagcc aggtcgcttt ctctttcggc
     8461 ttgttaagtc tggggctggc aatatcgtct tctgttgcgg gcaaattgca ggaacgtttt
     8521 ggtgttaaac gcgtcaccat cgcttccggc attttgctgg gattagggtt cttcctgaca
     8581 gcgcattctg acaacctgat gatgctgtgg ttaagcgccg gtgtgctggt cggtctggcg
     8641 gatggcgcgg gttacctgct gacgctctct aactgcgtga aatggttccc ggagcgtaaa
     8701 ggtctgatct ccgcgttcgc tatcggttct tatggtctgg gtagcctggg tttcaaattt
     8761 atcgacacgc agctgctgga aacggtcggt ctggaaaaaa cctttgtgat ttggggagcg
     8821 attgcgctgg tgatgattgt tttcggcgca acgttaatga aagacgcacc aaaacaggaa
     8881 gtgaaaacca gcaatggtgt ggtggagaaa gattacacgc tggcagagtc gatgcgtaaa
     8941 ccgcagtact ggatgttagc ggtaatgttc ctgactgcct gcatgagcgg cctgtatgtg
     9001 attggggtag cgaaagatat cgcccaaagt ctggcacacc ttgatgtggt ttctgcagcc
     9061 aatgcagtca ctgttatttc catcgccaac ctttcaggtc gtctggtgct gggcattctg
     9121 tctgacaaaa tcgcccgtat ccgtgttatc accattggtc aggtgatttc gctggtgggt
     9181 atggcggccc tgctgtttgc accattgaat gcagtgacgt tctttgcagc gattgcctgt
     9241 gtggcattta actttggcgg cactattacc gtctttccgt cactggtcag tgagttcttc
     9301 ggcctcaata acctggcgaa aaactacggt gtgatttacc tcggtttcgg tatcggtagc
     9361 atttgtggtt cgattatcgc ctcactgttt ggcggcttct atgtgacgtt ctacgtcatc
     9421 tttgctctgc tgattctgtc attggcgctt tctacgacga ttcgtcagcc agagcagaaa
     9481 gtattgcgtg aggcgcatat ttagccagaa actacagctt ttgaacgaaa gggggaggaa
     9541 atcctcc
//
LOCUS       DAZUCE010000194         9417 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZUCE010000194 DAZUCE010000000
VERSION     DAZUCE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.194"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            132..1196
                     /gene="ulaG"
                     /locus_tag="ACHY03_003960"
     CDS             132..1196
                     /gene="ulaG"
                     /locus_tag="ACHY03_003960"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFS3907138.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSVDMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1304..2059
                     /gene="ulaR"
                     /locus_tag="ACHY03_003961"
     CDS             1304..2059
                     /gene="ulaR"
                     /locus_tag="ACHY03_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFS3907139.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2056..2805)
                     /gene="yjfP"
                     /locus_tag="ACHY03_003962"
     CDS             complement(2056..2805)
                     /gene="yjfP"
                     /locus_tag="ACHY03_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFS3907140.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2987..3316
                     /gene="bsmA"
                     /locus_tag="ACHY03_003963"
     CDS             2987..3316
                     /gene="bsmA"
                     /locus_tag="ACHY03_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFS3907141.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3465..3740
                     /gene="yjfN"
                     /locus_tag="ACHY03_003964"
     CDS             3465..3740
                     /gene="yjfN"
                     /locus_tag="ACHY03_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFS3907142.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3857..5482)
                     /locus_tag="ACHY03_003965"
     CDS             complement(3857..5482)
                     /locus_tag="ACHY03_003965"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFS3907143.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5566..6729)
                     /locus_tag="ACHY03_003966"
     CDS             complement(5566..6729)
                     /locus_tag="ACHY03_003966"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFS3907144.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPQFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6732..7370)
                     /locus_tag="ACHY03_003967"
     CDS             complement(6732..7370)
                     /locus_tag="ACHY03_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFS3907145.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7380..7778)
                     /locus_tag="ACHY03_003968"
     CDS             complement(7380..7778)
                     /locus_tag="ACHY03_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFS3907146.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7796..8455)
                     /locus_tag="ACHY03_003969"
     CDS             complement(7796..8455)
                     /locus_tag="ACHY03_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFS3907147.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8506..9204)
                     /locus_tag="ACHY03_003970"
     CDS             complement(8506..9204)
                     /locus_tag="ACHY03_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFS3907148.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9223..>9417)
                     /locus_tag="ACHY03_003971"
     CDS             complement(9223..>9417)
                     /locus_tag="ACHY03_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFS3907149.1"
                     /translation="TFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLE
                     ITSLVDNALDLAEITEEYSH"
BASE COUNT         2438 a         2491 c         2259 g         2229 t
ORIGIN      
        1 tcaaaatcaa caacaacttc cctggcaaaa agtcaaaaaa cggtcattgc cttcctgaaa
       61 aaatagtgtc aggattaatc aaaaccaatc acatattgat tcgaattgaa ctatcatgaa
      121 ggtaaatggc gatgagtaaa gtgaaaagta ttacccgtga atcctggatc ctgagcactt
      181 tcccggagtg gggtagctgg ctgaatgaag aaattgaaca agagcaggta gctcctggta
      241 cattcgcgat gtggtggctc ggttgtaccg ggatctggct gaaatcggaa ggcggtacca
      301 acgtctgcgt cgatttttgg tgcggtactg gcaaacaaag tcacggtaac ccgctgatga
      361 aacagggtca ccagatgcag cgtatggctg gcgtgaaaaa actgcagccg aacctgcgta
      421 ccaccccgtt tgttcttgat ccgtttgcga ttcgccagat cgacgcggta ctggcgactc
      481 acgatcacaa cgatcatatc gacgttaacg tcgctgctgc cgtgatgcag aactgtgctg
      541 atgacgtacc gtttatcgga ccgaaaacct gtgtggattt gtggattggc tggggcgtac
      601 cgaaagagcg ttgcatcgtg gtcaaaccgg gcgatgtagt aaaagtgaaa gacattgaaa
      661 ttcatgcact tgatgctttc gaccgtactg cactgatcac cctgcctgcc gatcaaaaag
      721 cggctggcgt actgccagat ggcatggacg atcgcgcggt gaactacctg ttcaaaacgc
      781 ctggcggctc cctgtatcac agcggcgact cccactactc taactattat gcgaagcacg
      841 gtaacgaaca tcagatcgac gtggcgttag gatcgtacgg cgaaaacccg cgcggtatca
      901 ccgacaaaat gaccagcgtc gatatgctgc gtatgggcga agcgctgaat gcgaaagtag
      961 tgatcccgtt ccaccacgat atctggtcaa acttccaggc cgatccgcaa gagatccgcg
     1021 tgctgtggga gatgaaaaaa gatcgcctga aatatggctt taagccgttt atctggcagg
     1081 tgggcggcaa atttacctgg ccgctggata aagacaactt cgagtaccac tatccgcgcg
     1141 gtttcgatga ttgcttcact attgaaccgg atctgccgtt caagtcattc ctgtaatctg
     1201 tcgtttatac cggatagcgt ggagaagttc gcgctatccg gcgtagtatt tcctggctat
     1261 ttcaaatatc atctaaaaaa atcaaatttt atcggaatag ctcatgactg aagcacaaag
     1321 acatcaaatc ctcctggaaa tgctcgcaca attgggcttt gtgaccgttg agaaagtcgt
     1381 tgagcgtctg ggaatttcgc ctgccactgc gcgacgcgat atcaataaac ttgacgaaag
     1441 cggcaaactg aaaaaagtgc gcaatggcgc agaagctatt acccaacagc gcccgcgctg
     1501 gacgccgatg aatctgcatc aggcgcagaa tcacgatgaa aaagtacgta tcgccaaagc
     1561 ggcatcacag ctggttaatc cgggcgaaag cgtagtcatc aactgcggct ccaccgcgtt
     1621 tctgcttggg cgggaaatgt gtggcaagcc agtgcaaatc atcactaatt atctaccgct
     1681 ggcaaattac ctgatcgatc aagaacatga cagcgtgatc attatgggcg gacagtacaa
     1741 caaaagtcag tccatcactt taagcccgca gggcagcgaa aacagtctct atgccgggca
     1801 ctggatgttt accagcggaa aagggctgac cgcagaaggg ttgtataaaa ccgatatgct
     1861 gacagcaatg gcagagcaga agatgctgag cgtggtaggg aaactggtgg tactggttga
     1921 tagcagtaag attggcgaac gcgcgggaat gctttttagc cgtgccgatc aaatcgatat
     1981 gcttatcacc ggcaaaaatg ctaacccgga aatcctgcaa caactggaag cgcaaggggt
     2041 cagcattctg cgtgtttaaa gatgctggcg gaaaaatgtc acggcagcat ctaacgcctc
     2101 aggcgtaatg cggtgacgca cgcctggctg ccatgaacag gttagcagtt tatcccgtcc
     2161 cgtctcgctt aaggcctgct gcaaacgtag tgattcgtcg gcaggcacaa cgtcatcgtc
     2221 gaggccatgc cacagcagta gaggtctgtc actaagttgt tccaggtggt ttgtcgcttc
     2281 ccactctgcc agcggcgcga caatgttatt gaattcattc tgctgtgctg tcgtttcagg
     2341 tatcagcggt ggaaacagtg aacgggcgag tgatgtaaaa tagcccgatc ccatcatgct
     2401 ggcggtacat ttcaccgtgg ggtggcgagc ggtaattccc agtgccgtca tcgcgcccat
     2461 cgacgcgcca ccgactgcca gacggtcatc aagcagccag ttttcttcgg ctattgccgc
     2521 acgtaaagta gtgaattcct gcatactttg tagcaagatt tgccagaatt gatttaaccg
     2581 ccgcgctgcg tcaccactaa aacgactacc gtgatcgggc gcatccggca tgatcacccg
     2641 caaaccagcc tgcgccagcg caacggcaaa atagctatac accagactgg acgaagtaaa
     2701 gccgtgataa aaaatcacgc acggtaacgg ggtatctttt tgtccgacag gataagcatg
     2761 aagaacggga atatctgcca gctcgcgtga ttctatttca atcatctgtc tctccttttt
     2821 gtctggtatg acatgaagaa atccttagca caagtttctg ctggcggtat tagttcataa
     2881 tgttgagatg tgggttacgc tttcgtcacg ttttcattcg aaaatcgcgt accaggtaac
     2941 aattcgggaa cattccccca aagttaaatt aataaggcac tacactatgg ttagcaggaa
     3001 acgtaatagc gttatttacc ggtttgccag tttattattg gtgttgatgt taagtgcctg
     3061 tagcgcactg caaggtacgc cacagccagc accgccagta acggaccatc cgcaagagat
     3121 tcgccgcgac cagacgcaag ggttacagcg aataggtagc gtaagcacga tggttcgggg
     3181 ttctccggat gacgcattag cagaaatcag agcgaaagct gtcgctgcaa aagctgatta
     3241 ttacgttgtc gtcatggtgg acgaaaccat cgtgacagga cagtggtatt cacaagccat
     3301 tttgtaccgt aaataaatca gacaacttac atagtcagct ttacattgct ttgcgtccct
     3361 atgcgtttac ctgtgcacaa taaattaccg gctggaagcc tgggcgttta ttaccgcgat
     3421 ggaatgccct gcaacatgtg gggaaatgaa aaatggagct gacgatgaaa caattacttg
     3481 cctcaccctc gctgcaatta gtgacttatc ctgcgagcgc cacggcacag tctgcagaat
     3541 tcgccagtgc tgactgcgtg acgggcctga atgaaatagg tcagatctca gttagcaata
     3601 tttcagggga tccgcaggac gtggaacgta tcgtggcgtt aaaggccgat gaacaaggtg
     3661 cgtcatggta ccgcattatt accatgtacg aagaccagca gcctgataac tggcgtgtac
     3721 aggcgatact gtatgcgtga gtgtcatcta cagcaaatga acaatgccgg atgcggcgcg
     3781 agcgccttgt ccggcctgca attttcacat atttcattga gttaatcgaa accagcgtcg
     3841 catcagtcgt atacgcttac acacacactc cccccgtcgc ccgcagcaat aaatcattct
     3901 ggatctgctc tgacaaccgt acgccgccgc gcgtatctaa catcacctga caccacgcct
     3961 gcgccattgg cggagaggca tatttcaaca tttgcgcacc gcagcccagc aggaatagct
     4021 gatgagtaat ctctcgcccc agttcttcag ctggcttacg cagctgctgc tgtaaacgac
     4081 gaaccgcgcg atcaaaatag cgatcctgcc ctttcacttc cacaaatgct tccgacaata
     4141 agtcgtatac gcccgcttgc ttattgagaa cgcgcagcac atccaggcac ataatattgc
     4201 cggaaccttc ccaaatactg tttaccggca tctcccggta aagccgcggc agctcgctct
     4261 cctcgcaata accaatgccg cccagcacct ccatcgcttc ggccacaaac ggcatacctc
     4321 gtttgcagat cacaaatttt gccgcaggcg taaataaacg cgcccacagg gcttctttgg
     4381 catcggcacg ccggtcccac gctcgcgcaa gacgaaacag caacgccgtt tgcccttcaa
     4441 gctgaagtgc catgcgactt aagacatgac gcataagggg ctgttggatc aatggattac
     4501 caaaaacatg gcgttgatgt gcatgataaa tcgccagcga aaatgcacgg cgcatcatgg
     4561 catggctacc cagggcgcaa tcaaaacgcg tcatcccccc cattttcagg atcagacgaa
     4621 ttccttcccc ttcctgcccc aataaccagc caatggcatc ctgaaactcc acttcgcaac
     4681 tggcgttaga gcgattaccc agcttatctt tcagccgctc gaggcgaatc gcgttgcgtt
     4741 gcccgtcagg caaaaagcgc ggcacaaaaa agcaggacag accgcccgcg gtctgcgcca
     4801 gcaccagatg cgcatcgctt tgcggcaccg agaaaaacca tttatgcccc accagccgat
     4861 aagagccatc ttccaaacgc tctgcacggg tggtgttgct catgacatcg gaaccgccct
     4921 gcttttccgt cattcccatg ccaatcaaca aaccgcgttt ttgcccacca ggcaataagt
     4981 gagaatcgta gcgatcgctc agcagcggcg tggtccagtc ctgaaacggc gtgggtaaca
     5041 tctgtaacaa caatggcgtg gcggcaaagg tcatggttat cggacataac gaccccgcct
     5101 caacctgtgc gtgcaacata aaacgcgccg cgcgcgccac aaatgcgccg gagcgagcgt
     5161 cttcttccca ggcaagattg tgcacccgat tggtacatag cgcctgcatc agcaggtgcc
     5221 aggcggggtg aaaacgcaca tcgtccagac ggcgtccttg cgcatcgtag cgcaataatt
     5281 caggcggatt cacattcgcc agccgcccca gttcaaggga ttcagccgtt cctaactgct
     5341 gaccaatact ggctagaaaa tcgctatccc agccagcacc ttcacgcgtt accgcttcgc
     5401 aaagcgcacc atcagacagg tataagttgc tgttatttaa tggtataggt tgattaaaaa
     5461 cggtgtgagt ttgccagtgc actgtgtctc cctccatcaa tggcagtcac cattagtatg
     5521 gtcactgcca tggattcatg acagaaagag attgctaaaa cattcttatc cagcaatgta
     5581 atgcggaacg aaacgtgagg tgtctttggt gatcagtgtg ttatcttcac ggatccccat
     5641 tccgcacgct tcatcatcga caatccagct accgatgagt gtgtagctat cgccaaattg
     5701 cggcagaggt tgaaacgcct ggtagatcat cggttcatcg gcgtaatcac catcagcgtg
     5761 gtcaacgacg ttattctggc cgtcgaaaat ggtgacattt ccgccttcgc gcgagtagat
     5821 cggtttacgc acatagcttt cgccagcggc aatctgcggt ttttcaccct caaaccagga
     5881 tgcaagaagg ttaggatgac cagggaagaa gcgccaaagc agcggcatta gccctttatt
     5941 actcaagata cttttccata acggctccac ccattgctcg cgacgcttgc gcagcagcgg
     6001 accattatcg tcacgcatca tccactccag cggatacagc ttaaatgcac gctggatgac
     6061 attatcatca agatcggtca gtacgccgcc gacgcccaaa ccgagatctt caatgtagat
     6121 aaaccgcgac tcctgccctg cctgctgggc gcagtcctgc aagtacagca cggtactcct
     6181 gtcttcatcg gtgtcctgac agcagcaaaa ataaaacggc tcccgactgt aaagctcact
     6241 aaagcgcgaa atcaggcgtt cctgaatggc attgtactga tcggcatcac gcggaataat
     6301 gccgctgcgc cgggcatcct ctagccacaa ccactggaaa taagccgact catacaacga
     6361 ggtcggcgta tcggcgttgt actccagcag cttcaccggc gcattgccac accaggcaaa
     6421 atccatccgg ccatacagcg aaggatcgcg tgcgcgccag ctttcagcga tcacatccca
     6481 gtacaacggc gggatcgcca gttgcgtcag gatctcttcg tctttaaccg cgcgatccac
     6541 cacctcaagg cacatctgat gcagttccgc agtcggtttt tcgatctgct cttcaatctg
     6601 gcgcagagta aaacggtaag cccgactctc gtcccaatag atttcattgt cgatgatatg
     6661 aaagtcgaaa ccgttgtcag cggcgatttg gtccagatcc cgccgcacag gaacgttgtg
     6721 tctcagcatg attagcctcc ccaatgccca cgggcgctgg aagaacgacc atagccgccg
     6781 cgcgaaacgg tcgacgcttt tctggtggta aagccgcctg aagagtaaga ttcttttttg
     6841 ccagaaccgg agcgccagga gtaatcacca gaagtgttgc gccagacggg gcgggaagca
     6901 aatgaggatc cgccgctgtt atagacgaac ggctcatcgc gatctttacg aataacccgg
     6961 cttaacaaaa atccagaaac gaccgggatc caactctgtt caacattgtc gtagtagcaa
     7021 ttttcgtact tagactgaca gttctgctga gtcatattct tcggaacatc ggcataaaat
     7081 gccgctttgg cgttgttcca gccacgcgcg caaatgtcgg cattattacc gtcatcaata
     7141 caatcctgca cagtcgagta aaacgttccg tcgccgtcgt tatcgacatc gctactttca
     7201 ctccatcctt tgactattaa aaaagcggca ccgcccatta gcgccaacgt taagtatttt
     7261 tgtgcgtagc gattgcgacg cggttcaaaa ggattattcg gtctaccaat gcgactgatc
     7321 gctccgtgac caattttact gttatttctt gatttgcgtt ttctggccat gatcgtccat
     7381 taccatgtca tacaagctgc gttaaaaata ccgccagcca gcgcggcggt tcccataaac
     7441 attcctgctg cggtattgtg attaataatt ttttcgctta atgcgggcat ataaagtctg
     7501 acaccagcaa aaacgagtaa ctgaatcacc agtgcaatcc cgccccaggc gaaatagtct
     7561 ggaatactca ccgcattgat tgccgcactg gataagggga tcacgtaacc caacaatgta
     7621 ccgctgaacg ccagtgacgc tgcggtatta ttgtttttga ttaattgcca ttcattgtgc
     7681 ggtgtaattt tggagtagat aaacagaaaa ataatcacca tcgccacgcc aataaaaaaa
     7741 taggcactaa aggcaagaag tgaatccagt atatgcattg taaacgtcct tgtaattaac
     7801 caataatatg aagtgatgtc agcgggatat cgacacccaa cgcgcgcgaa aataaccact
     7861 ctggctcacc gagatcgtta aaagattcct caccatttag cagcaaatat tcgtaagtat
     7921 cttcggtcac ttgccgctgg tagcccatgg taaaattatg gacttcccat ttagcatgat
     7981 tttgattttc tactttttcc agcatgtaga cgggttcgat atttcccggt tcttcgctgt
     8041 taaaaaatcg ctgccagcgt ttttcctgcc agtttaaggt cattgccccc atcgttttgg
     8101 cgttgatcgc ctcgcgccag tgactctctt tactgatacc gtagctctct tcatagacaa
     8161 agagcttgat atcatcaatg tcatcaatat cttcgccgcc ggtggtattg atttgcagaa
     8221 actcatcgcc tgaagtgtag tagcggaaaa tctggctacc gccgcccaga tcgatgtgac
     8281 tgacagcggc taccgtaaat tcctcacccg gcagcgcaat cagcaattca tcttccagca
     8341 aacgaaacgc taacgtgtcg agcgtaaagc cactattgag atgaagtccc agcggaccgc
     8401 gagcgatagc aggcttatta tccttgccaa acagacgctg gaaaaaacca gacatatttt
     8461 atccttaaaa tagttgccag ccttgcagtg aggctggcaa aaaaattact cgccctgttg
     8521 gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc caattccggc
     8581 ttccgccagc ttttcgtcaa ggtcgcgacc gtctgcgact ttctccaact gtgcggcagc
     8641 atccaggcga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg attctgccgc
     8701 tgtcgaaacg ctggaagatg cgccaacggt agaggttgtt acagcctgtt gtgcacgctg
     8761 catggcttca gtggctttaa cgacttccat ttgctgctca aactgagcga tacgctgcgc
     8821 tgtcgctgta actgcttttt ccacgccatc acgggagact tccagattcg acaacacttg
     8881 ctcttcagcg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt cgttaatcaa
     8941 cgacggatta acgttcttgc ttaacgcttc cagcgcacgg gcttccagac tggctttgcg
     9001 ctcacgtaaa tctttcagct tatcgtgact taatttcacc cgcgccagca gatcaacgcg
     9061 agattttccg gctttatcga gttcagcttt agcgtcacga atatgctgtt ccagcatgcg
     9121 cacaccctgg gtttcttcaa tggactcttc cgcctgggag ataaacgatt tccccagcgt
     9181 aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg aatattcttc
     9241 tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa gcaggatatc
     9301 ttcaagagaa gatttaagcg ataacgcccc gaaaacaatg taatactctt cctgttgcac
     9361 gctggagatt cctaccgatg acaacggcat catcttctga tttcttaata agaaggt
//
LOCUS       DAZUCE010000195         9249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZUCE010000195 DAZUCE010000000
VERSION     DAZUCE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.195"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            59..1474
                     /gene="tnaA"
                     /locus_tag="ACHY03_003972"
     CDS             59..1474
                     /gene="tnaA"
                     /locus_tag="ACHY03_003972"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFS3907150.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1565..2812
                     /gene="tnaB"
                     /locus_tag="ACHY03_003973"
     CDS             1565..2812
                     /gene="tnaB"
                     /locus_tag="ACHY03_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFS3907151.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2944..4119
                     /gene="mdtL"
                     /locus_tag="ACHY03_003974"
     CDS             2944..4119
                     /gene="mdtL"
                     /locus_tag="ACHY03_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFS3907152.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQM
                     LFLAAGITLAVSPSHAISLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4094..5053
                     /gene="yidZ"
                     /locus_tag="ACHY03_003975"
     CDS             4094..5053
                     /gene="yidZ"
                     /locus_tag="ACHY03_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFS3907153.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRDTIKNLYASMA"
     gene            5210..5959
                     /locus_tag="ACHY03_003976"
     CDS             5210..5959
                     /locus_tag="ACHY03_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFS3907154.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            5981..6547
                     /gene="yieF"
                     /locus_tag="ACHY03_003977"
                     /gene_synonym="chrR"
     CDS             5981..6547
                     /gene="yieF"
                     /locus_tag="ACHY03_003977"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFS3907155.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6601..7938)
                     /gene="adeP"
                     /locus_tag="ACHY03_003978"
     CDS             complement(6601..7938)
                     /gene="adeP"
                     /locus_tag="ACHY03_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFS3907156.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8105..8770
                     /gene="yieH"
                     /locus_tag="ACHY03_003979"
     CDS             8105..8770
                     /gene="yieH"
                     /locus_tag="ACHY03_003979"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFS3907157.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMIQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPINKMQHSLGKLKMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELAQLPALWKARGWN
                     ITR"
     gene            complement(8907..>9249)
                     /locus_tag="ACHY03_003980"
     CDS             complement(8907..>9249)
                     /locus_tag="ACHY03_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFS3907158.1"
                     /translation="NYQEINTSIKDYDDLLREIKELTISRNKSLEDKRTLFDNKRDNW
                     NSIEVQDEVNALNAKIVDCDDKIRSKLTIVRNNRLENQYKDDKNISNAMRNILDWFER
                     YPDIVQNITQA"
BASE COUNT         2276 a         2316 c         2291 g         2366 t
ORIGIN      
        1 attgcaaatg taatattcac agggatcact gtaattaaaa taaatgaagg attatgtaat
       61 ggaaaacttt aaacatctcc ctgaaccgtt ccgcattcgt gttattgagc ctgtaaaacg
      121 taccactcgc gcttatcgtg aagaagcaat tattaaatcc ggtatgaacc cgttcctgct
      181 ggatagcgaa gatgtgttta tcgatttact gaccgacagc ggcaccgggg cggtgacgca
      241 gagtatgcag gccgcgatga tgcgcggcga cgaagcttac agcggcagcc gcagctacta
      301 tgcgttagcc gagtcagtga aaaatatctt tggttatcaa tacaccattc cgactcacca
      361 gggccgtggt gcagagcaaa tctatattcc ggtactgatt aaaaagcgcg agcaggaaaa
      421 aggcctggat cgcagcaaaa tggtggcgtt ctccaactat ttctttgata ccacgcaggg
      481 ccatagccag attaacggct gtaccgtgcg taacgtctat atcaaagaag ccttcgatac
      541 gggcgtgcgt tacgacttta aaggcaactt tgaccttgag ggattagaac gcggtattga
      601 agaagttggt ccgaataacg tgccgtatat cgttgcaacc atcaccagta actccgcagg
      661 cggtcagccg gtttcactgg caaacttaaa agcgatgtac agcatcgcga agaaatacga
      721 tattccggtg gtaatggact ctgcacgctt tgctgaaaac gcctatttca tcaagcagcg
      781 tgaagcagaa tacaaagact ggaccatcga gcagatcacc cgcgaaacct acaaatatgc
      841 cgatatgctg gcgatgtccg ccaagaaaga tgcgatggtg ccgatgggcg gcttgctgtg
      901 catgaaagac gacagtttct ttgatgtgta caccgagtgc agaacccttt gcgtagttca
      961 ggaaggcttc ccgacgtatg gcggcctgga aggcggcgcg atggagcgtc tggcggtagg
     1021 tctgtatgac ggcatgaatc tggactggct ggcttatcgt attgcgcagg tgcagtatct
     1081 ggtcgatggt ctggaagaga ttggcgttgt ctgccagcag gcgggcggtc acgcggcatt
     1141 cgttgatgcc ggtaaactgt tgccgcatat cccggctgat caattcccgg cacaggcgct
     1201 ggcctgcgag ctgtataaag tcgccggtat ccgtgcggta gaaattggct ctttcctgtt
     1261 aggccgcgat ccgaaaaccg gtaaacaact gccatgcccg gcagaactac tgcgtttaac
     1321 cattccgcgc gcaacatata ctcaaacaca tatggacttc attattgaag cctttaaaca
     1381 tgtgaaagag aacgcggcga atattaaagg gttaaccttt acctacgaac cgaaagtatt
     1441 gcgtcacttc accgcaaaac tgaaagaagt ttaattgaca ctacagagtg gctataagga
     1501 tgttagccac tctcttaccc tacatcctca ataacaaaaa tagccttcct ctaaaggtgg
     1561 catcatgact gatcaagctg aaaaaaaaca ctctgcattt tggggtgtta tggttatagc
     1621 aggtacagta attggtggag gtatgtttgc tttacctgtt gatcttgccg gtgcctggtt
     1681 tttctggggt gcctttatcc ttatcattgc ctggttttca atgcttcatt ccgggttatt
     1741 gttattagaa gcaaatttaa attatcccgt cggctccagt tttaacacca tcaccaaaga
     1801 tttaatcggt aacacctgga acattatcag cggtattacc gttgccttcg ttctctatat
     1861 cctcacttat gcctatatct ctgctaatgg tgcgatcatt agtgaaacga tatcaatgaa
     1921 tttgggttat cacgctaatc cacgtattgt cgggatctgc acagccattt tcgttgccag
     1981 cgtattgtgg ataagctcgt tagccgccag tcgtattacc tcattgttcc tcgggctgaa
     2041 gattatctcc tttgtgatcg tgtttggttc tttcttcttc caggtcgatt actccattct
     2101 gcgcgatgcc accagctcca ctgcgggaac gtcttacttc ccgtatatct ttatggcttt
     2161 gccggtgtgt ctggcgtcat ttggtttcca cggcaatatt cccagcctga ttatttgcta
     2221 tggcaaacgc aaagataagc taatcaaaag cgtggtcttc ggttcgctgc tggcgctggt
     2281 gatttatctc ttctggctct attgcaccat ggggaatatt ccgcgagaaa gctttaaggc
     2341 gattatttcc tcaggcggca acgttgattc actggtgaaa tcgttcctcg gcaccaaaca
     2401 gcacggcatt atcgagtttt gcctgctggt gttctccaac ttagctgtcg ccagctcgtt
     2461 ttttggtgtc acgctgggat tgttcgatta tctggcggac ctgtttaaga ttgataactc
     2521 ccacggcggg cgtttcaaaa ccgtgctgtt aaccttcctg ccacccgcgt tgttgtatct
     2581 gatcttcccg aacggcttta tttacgggat cggcggtgcc ggactgtgcg ccactatctg
     2641 ggcggtcatt atccccgcgg tgctggcaat caaagctcgc aaaaagtttc ccaatcagat
     2701 gttcacggtc tggggcggca atcttattcc ggcgattgtc attctctttg gtataaccgt
     2761 aattttgtgc tggttcggca acgtctttaa cgtgttacct aaatttggct aaatccttca
     2821 agaagccagc cattcgctgg cttcttgcct ctcaggaaat cacttatgtc caaatggcaa
     2881 ctcgcctgat cctccttcac cacgtatgct ttgcgtcacc ttactatcag gacgctttag
     2941 cccatgtccc gctttttgat ttgtagtttt gccctggttt tactttatcc cgctgggatt
     3001 gatatgtacc tcgttggttt accgcgcatc gccgccgatc tcaatgccag cgaagcgcag
     3061 ttacatattg cgttctccgt ttatctggca ggtatggcag ctgcgatgtt atttgccggt
     3121 aaagtggccg atcgttcagg gagaaaaccg gtcgccatcc ccggcgcggc gctatttatt
     3181 atcgcctcgg tgttctgttc actggctgaa accagcacgt tatttcttgc agggcgcttt
     3241 ctacaggggt tgggcgcggg ctgttgttac gtggtggcat tcgccatttt gcgcgacacg
     3301 ctggatgatc gacgtcgggc aaaagtgctg tcgctactca acggcattac ctgcatcatt
     3361 ccggtgttag cgccggtgct cggtcatctg attatgctta aattcccgtg gcagagtctg
     3421 ttctggacga tggcaataat gggcatcgcg gtactgatgt tgtctttgtt tattttaaaa
     3481 gaaacgcgcc ctgccgcccc cgcagcttcg gacaaatcac gagaaaatag cgagtcgctg
     3541 cttaatcggt ttttcctcag ccgtgttgtt atcaccaccc tcagcgtttc ggtgatcctc
     3601 accttcgtca acacgtcgcc agtattgctg atggaaatca tgggttttga gcgcggagaa
     3661 tacgccacca ttatggcgtt gaccgctggc gtcagcatga ccgtttcatt ctcgacgcca
     3721 tttgcgctgg gaatctttaa gccacgtacg ttgatgatca cttcgcagat gttattcctg
     3781 gcggcgggga tcacccttgc cgtttcacct tcccatgcga tttctctgtt tggcatcacg
     3841 ctgatttgcg ccggtttctc ggttggtttt ggtgtggcga tgagtcaggc gttagggccg
     3901 ttttcattac gcgcgggcgt agccagctcg accttaggta ttgcgcaggt ttgcggttcg
     3961 tcactgtgga tttggctggc agcagtggtt ggtatcggcg catggaatat gctgatcggg
     4021 attctgattg cctgtagcat agtgagcctg ttgctgatta tgttcgtcgc gcctggacgc
     4081 cccgttgccg ctcatgaaga aatccatcac cacgcttgat ctcaatctgc tgctttgtct
     4141 gcaactgctg atgcaggagc gcagcgtaac caaagcggcg aagcggatga acgtgacccc
     4201 ttcggcggtg agtaagtcgc tggcaaagtt aagagcgtgg tttgacgacc cgctctttgt
     4261 gaactcaccg ctggggctgt cgcccacacc gctaatggtc agcatggagc aaaatctggc
     4321 ggagtggatg caaatgagca acctgctgct ggataaaccg catcaccaga caccgcgcgg
     4381 cctgaagttt gagctggcgg cagaatcacc gctgatgatg attatgctta atgcgctgtc
     4441 gaaacggatc taccaacgtt acccgcaggc gaccatcaaa ctacgtaact gggattacga
     4501 ttccttagat gccattactc gtggtgaagt ggatatcggt ttttccggtc gcgaaagcca
     4561 tcctcgctcg cgggagctat taagctcgct accgttagcc attgattatg aagtgttgtt
     4621 tagtgatgtg ccctgcgtct ggttacgcaa agatcatccg gcactgcatg aaacgtggaa
     4681 tctggacacc tttttacgtt atccgcatat cagcatttgc tgggaacaga gcgacacctg
     4741 ggcgctggac aatgtgttac aggagctggg gcgcgaacgc acgattgcta tgagcctgcc
     4801 ggaattcgag cagtcactgt ttatggcagc gcagcccgac aatctgctac tggcgaccgc
     4861 gccgcgctac tgtcagtact acaatcaact ccaccaactg ccgttggttg ctcttcctct
     4921 cccgtttgac gaaagccagc aaaaaaagct ggaagtccct tttaccctgc tgtggcataa
     4981 acggaacagc cataatccga agatcgtctg gttacgggat accattaaaa atctttacgc
     5041 gtcgatggca taaccgaatc gtatgaaacg cgacccgatt tcacaataaa atgtaaaaaa
     5101 gttgtaataa gcttgtctga atcgaacttt tagccgcttt agtctgtcca tcattccagt
     5161 aaatgattac tcttgtattc ataatggacc attaagcatg gagcgaaaaa tggcgactca
     5221 ctttgcccga gggattttaa cggaaggaca tctgatttct gttcgtctcc cctcccagtg
     5281 tcatcaagaa gcccgaaaca ttccccctca tcgtcaaagc cgttttctgg cgtccagagg
     5341 tttactcgca gaactgatgt tcatgctgta tggcattggc gaattgccgg aaatcgtcac
     5401 cctgccgaaa ggtaaaccgg ttttcagtga taaaaatttg ccttcgtttt ctatttccta
     5461 tgccgggaat atggttggcg tggcgttaac aaccgaaggt gaatgtggcc tcgatatgga
     5521 actacagcgt gcgacgcgcg ggtttcatag cccacacgcg cccgataacc acaccttttc
     5581 cagcaatgaa tcgctatgga tcagtaaaca aaacgatcct aacgaagcgc gggcgcagct
     5641 catcacgctg cgccgaagcg tgctaaaact aaccggtgat gttttgaatg acgatccgcg
     5701 cgatctgcaa ctactgccca ttgccggacg cctgaaatgt gctcatgtaa atcatgtaga
     5761 agcgttatgc gacgcggagg acgtgctggt gtggtccgtg gcggtcacgc ccacgattga
     5821 aaagctcagt gtctgggagt tagatggcaa acacggctgg aaaagcctgc ccgatattca
     5881 cagccgcgcc aacaatccta ccagccggat gatgcgtttt gcccagctct ctaccgtgaa
     5941 ggctttttcg ccaaattgat agacaaccac aggagtcatc atgtctgaaa aattgcaggt
     6001 ggttacgtta ctggggagcc tgcgcaaagg ctcatttaat ggcatggttg cacgtaccct
     6061 gccgaaaatt gctccggcga gcatggaagt caatgcgtta ccttccattg ccgacattcc
     6121 cctgtatgac gctgacgtac agcaggaaga aggttttcca gcaacggttg aagctctggc
     6181 ggaacagatc cgtcaggctg acggtgtggt gatcgtcacg ccggaatata actattcggt
     6241 accaggtggg ctgaaaaatg ccatcgactg gctttcccgc ctgccggatc aaccgctggc
     6301 cggtaaaccg gtgttgattc agaccagctc aatgggcgtg attggcggcg cgcgctgtca
     6361 gtatcacctg cgtcagattc tggttttcct cgatgcaatg gtgatgaaca agccggaatt
     6421 tatgggcggc gtgattcaga acaaagttga tccgcaaacc ggagaagtga ttgatcaggg
     6481 tacgctggac cacctgaccg ggcaattgac cgcatttggt gagtttattc agcgagttaa
     6541 gatctaaata aaaaacccgc cagcaatcat gcatggcggg tttttaatgc gctatcgatt
     6601 ttaatgagcg tcgataaata caatcttcag gataaacagc agcgcaacga tgattacgca
     6661 cgggctaagg tcacggagac gcccggtacc aatcttcatc acgcagtagg agataaagcc
     6721 cagcgcaata ccttcggtaa tcgagaagct gaacggcatc atcacagcgg taataaacgc
     6781 cggaacagat tcagtaagat cctgccagtt cacgcgagca agacttgagg tcatcaacac
     6841 gccaacgtaa atcagcgcgc cagctgcagc gtagcctggc accattcccg ccagcggcga
     6901 cagaaagata accagcagga acagcagacc aacaaccact gccgtcagac cggtacgacc
     6961 gccaaccgat acgccggaag aggactcaat ataagccgta acggaagaag taccgataaa
     7021 cgaaccggtc acggaagaga tactgtcgac atacagcgcc tgcttcatgc gcgggaattt
     7081 ccccttctca tctgccagac ctgctttatc ggtcacgcca atcaacgtac cggaggagtc
     7141 aaacaggttg accagcatga aagagaaaat cacccctgcc agcccgaggt taaacgaccc
     7201 ggctaaatct acatgaccca cgacagtcat tacgctcggc ggcgcagaaa cgatgccatt
     7261 gtagtggaca tcacccagca tccagcccag cagcgttgtc accacgatag aaaccagcac
     7321 cgctgcgtga atgttgcgtg aggccagaat agcaatgatg aagaagccga ggatccccag
     7381 cagcacgctg tgagaagtca gattaccgat gctgaccagc gtttccgggt tagcgacaat
     7441 cacacctgcg tttttcagcc ccatcatgcc aatgaacaga ccgataccgc tggtaatgcc
     7501 cacacgcaga ctcaccggaa tgttggcaat catccagtag cgaacgcgga aaatcgtcag
     7561 taatagcaga ccaatcgcgc cccagaagat tgcgcccatc ccgacttgcc acggcaagcc
     7621 catcgcctgc acaacgacaa aagcgaagaa cgcattcagg cccatagcgg gtgccagtgc
     7681 aactggcagg ttagcaaaca gccccatcat aatactgccg aatgcagcaa tcagacaggt
     7741 agtaacgaag acggcgctgg tatccatgcc agcaacgcca agaatttgcg ggttaacaaa
     7801 aacgatgtaa accatcgtca ggaaggtggt aaaaccggcg atcacttcgg tccgtgccgt
     7861 cgtgccatgt tcgcgcagtt taaacacgcg ttccagcatc ccctggccag aagcctgggt
     7921 tgtgtgttgt tgactcatta tctatttccg aacaaggagg gaaaatccgt cgctatcgta
     7981 taccaaaatg agacaatatg gacggctgaa gataactttt tttcattgta tttgcttata
     8041 cggcaacgat tgcgtcatgc gaattggcat gacgaaaacg ttaaactaac gataaaggaa
     8101 agacatgtcc cagatagaag cggtattttt cgactgcgac ggtacgctgg tcgatagcga
     8161 ggtcatttgc tctcgcgcct atgtcacgat gttcagggaa tttggcattc acgttgacct
     8221 ggaggagatt tttacccgtt ttaaaggcgt aaaactctac gaaatcatcg acatcatcag
     8281 taaagaacag ggcgtgacga tgatccaggc cgatgccgag cacgtttacc gcgcagaagt
     8341 ggcgcgtctg tttgataccg aacttgagga catcgcagga gcgaacgaat tgctggcgag
     8401 cattaacgtg ccgatgtgcg tcgtctctaa cggtccgatc aacaaaatgc agcattcgct
     8461 gggtaagctc aagatgctgc actacttccc ggaaaaactg ttcagcggct acgatatcca
     8521 gcgttggaag cccgatccgg cattaatgtt ccatgcggca aaagcgatga atgtgaacgt
     8581 tgaaaactgc attctggtcg atgactccag cgccggcgcg caatcaggga tagatgccgg
     8641 aatggaagtg ttttacttct gcgctgaccc gcacaataaa ccgatcgttc acccgaaagt
     8701 caccaccttt accgagctgg cgcagctgcc tgcactctgg aaggcacggg gctggaatat
     8761 aacgcgataa tcgcctcctt acttcaacgc ttacacatca gtcacgctgg tttgagataa
     8821 aaaataccag gtgtctgatg agtaagcgtc tattccctat aaataattta tgtcgttatt
     8881 cgtcctgtca tccaccctct gtatggttaa gcctgggtta tattttgtac aatatcggga
     8941 taccgttcga accaatccag aatattacgc atcgcgttac tgatgttttt atcgtcttta
     9001 tattgattct caagtctatt atttcgaaca atagtaagct tagaacgtat tttatcatca
     9061 caatcgacaa ttttcgcatt caatgcattt acttcatcct gcacttctat actattccaa
     9121 ttgtcccttt tgttatcaaa taaagtcctt ttatcctcta atgatttatt acgacttatg
     9181 gttaactctt taatttctcg caataagtca tcgtaatctt taatggaagt atttatttcc
     9241 tgatagttt
//
LOCUS       DAZUCE010000196         9232 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZUCE010000196 DAZUCE010000000
VERSION     DAZUCE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.196"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            70..432
                     /locus_tag="ACHY03_003981"
     CDS             70..432
                     /locus_tag="ACHY03_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907159.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKGMGKTTEE"
     gene            complement(713..1171)
                     /gene="ybbJ"
                     /locus_tag="ACHY03_003982"
     CDS             complement(713..1171)
                     /gene="ybbJ"
                     /locus_tag="ACHY03_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFS3907160.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1168..2085)
                     /gene="qmcA"
                     /locus_tag="ACHY03_003983"
     CDS             complement(1168..2085)
                     /gene="qmcA"
                     /locus_tag="ACHY03_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFS3907161.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2231..2908
                     /gene="fetA"
                     /locus_tag="ACHY03_003984"
     CDS             2231..2908
                     /gene="fetA"
                     /locus_tag="ACHY03_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFS3907162.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2895..3674
                     /gene="fetB"
                     /locus_tag="ACHY03_003985"
     CDS             2895..3674
                     /gene="fetB"
                     /locus_tag="ACHY03_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFS3907163.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3737..4591)
                     /gene="cnoX"
                     /locus_tag="ACHY03_003986"
     CDS             complement(3737..4591)
                     /gene="cnoX"
                     /locus_tag="ACHY03_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFS3907164.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4652..5461)
                     /gene="ybbO"
                     /locus_tag="ACHY03_003987"
     CDS             complement(4652..5461)
                     /gene="ybbO"
                     /locus_tag="ACHY03_003987"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFS3907165.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5451..6074)
                     /gene="tesA"
                     /locus_tag="ACHY03_003988"
     CDS             complement(5451..6074)
                     /gene="tesA"
                     /locus_tag="ACHY03_003988"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFS3907166.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6045..6731
                     /gene="ybbA"
                     /locus_tag="ACHY03_003989"
     CDS             6045..6731
                     /gene="ybbA"
                     /locus_tag="ACHY03_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFS3907167.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6728..9142
                     /gene="ybbP"
                     /locus_tag="ACHY03_003990"
     CDS             6728..9142
                     /gene="ybbP"
                     /locus_tag="ACHY03_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFS3907168.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVNWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2095 a         2391 c         2542 g         2204 t
ORIGIN      
        1 gtgtgctaaa atgcgtttct ttgataaatc tcaattttta agaggtttac gccaggcagg
       61 aaaatttaaa tgaatgaatt tgagaagatt tttaatgaaa tgaatctgga ccgggcattg
      121 ctaccaatcc tttttcgttc gaaccggtcg acagtgtgga agtatttaag cggcgatagt
      181 acggctcctg cctctgccat gtcgctgatt atgctgttgc agttgatcca gaaacgaaac
      241 cccgatctgt tagccgaatg gttaacgctt tctgatttca ccattccgcc agaagtttac
      301 ctggaccagc ctgattactg gaaagggtgg gtgtataccc aacacaaggt caataaaaac
      361 gtcctggaat atctaaaaaa acactatccg gatgaagacc aaaaaggtat gggtaaaacc
      421 acagaagagt aaattgtcat tacccgccca catgccggat gcgcacagac tggcagtgtt
      481 agtaggcatg ataatacgcg tcagcgtcgc atcaggcatc ggcgcataac cgccggatgc
      541 ggcgtaaacg ccttatccgg cctacgattc gggcgcagac ttccaggcat gataatacgc
      601 gcccgcctcg catcaggtgt cggtgcacga ctgccggatg cggcgcaagc gccttatctg
      661 gcctacgatt cgggcatatt ttgtaggcat gataagacgc gtcagcgtcg catcaggcga
      721 tgaccgcacg gatgatcagc gttatccctt ctatcgcaat gacttcaaca tgcgtacctg
      781 caccgagatc ctcgctggcg ctgacaggcc atgaactgtc accgacgcgc atatgaccgc
      841 gcccgttgac cagcggagat tccagcacaa aacgtcggcc aatcagctgc tgcccgcgct
      901 ggtttaaatg actgtcgctg tgcttttgtt cgcgcacccg ccgcgacaac catttccacc
      961 acagccaggc ggcgagcagc gtcaggatgg caaacatcac cccttgccac tcccaaccca
     1021 gcggcaccag ccagaccacc aggccggtaa tcaccgctgc cacgccgctc cacaacaaat
     1081 aaccatttcc gcccagcatc tcggctgcca gcagcaaacc gccgagactg agccagaaaa
     1141 tatgtggatg aacgaccatt aactccatca tggctgagtc cgcttgttgg cgctgtcttt
     1201 caccagctcg gcaatcccgg caatcgaccc catcaggctg ctggcctcta atggcatcat
     1261 caccactttg ctgttactgg acgaaccgat ctgctgtaac gcttcggtgt atttctgcgc
     1321 tacgaagtag ttcaccgcct gaatatcgcc ggaggcaatg gcttcagaca ccattttggt
     1381 ggcgcgggct tctgcttcag cggaacgttc acgcgcttca gcctgtaaaa acgccgactg
     1441 acgttcgcct tccgctttca ggatttgcga ctgtttttca ccttcggctt tgaggatttc
     1501 cgcctgacgg atcccttccg cttcgaggat gtaagcgcgt ttggtacgtt ccgctttcat
     1561 ctgcgcgttc attgaagaga taagctctgc cggtgggcgc acgtcgcgaa tttcaatacg
     1621 ggtgacttta atcccccacg ggttggtggc ttcatcgaca atacgcagca ggcgtgagtt
     1681 gatgctgtcg cgctgagaga gcatttcgtc aagttccatt gaacccaaca cggtacggat
     1741 gttagtcatg gtcaggttga tgattgccag ctccagattg ctgacttcat aagccgcgcg
     1801 tggcgcgtca atcacctgaa taaaacagac tgcgtcgatg gtaacgttgg cgttatcttt
     1861 cgagataact tcctgggaag ggatatcgag cacttgctcc atcatattga tcttgcgacc
     1921 aatgcgatcc ataaacggca ccaccagact gagccccggc tgtaacgttt tggtatagcg
     1981 accaaaacgt tctaccgtcc attgatagcc ctgcggcacg attttgacgc ccgcgccgac
     2041 aatgaccagc gcgacaaaaa tgagaatcgg gataaagata agcatcggaa aaacctcctg
     2101 ttgtaccgtc cataatcagc aaaattgctg cttgattaaa caaattatac ctgattactg
     2161 aaagagagtt cccccttatt cctgcgaagg ataaactgtt tttagtaaaa atcagaaaaa
     2221 gggaacagcg atgcaggaaa atagtccttt gcttcagcta caaaacgtag gatatctggc
     2281 gggtgatacg aagattctta ataacatcaa tttttcgctg cgtgctggcg aatttaagtt
     2341 aattaccggt ccttctggtt gtggcaaaag tacgctgcta aaaatagttg cttcattgat
     2401 cagcccaacc agcggaacgt tactgtttga aggtgaggat gtcagcacac taaagccaga
     2461 aatctaccgc caacaagtct cttactgcgc ccagacaccg acgctgtttg gcgatacggt
     2521 atacgataat ctgatctttc cctggcagat ccgtaaccgg cagcctgacc cagccatttt
     2581 tctcgatttt ctcgaacgct tcgccttgcc ggacagcatt ttgacgaaga atatcgccga
     2641 gctatctggt ggtgaaaaac aacgcatctc attgattcgt aacctgcaat ttatgccgaa
     2701 ggttttattg ctggatgaaa taaccagtgc gctggatgaa agtaataaac ataacgtcaa
     2761 tgagatgatc catcgttatg tgcgcgagca aaatattgcc gtgctatggg tgacacacga
     2821 taaagacgaa attaatcatg cggataaagt gattacactg caaccgcatg ccggagaaat
     2881 gcaggaagca cgctatgaac tcgcataata ttactaacga atcattagca ctggcattaa
     2941 tgctggtggt ggtggcaatc ttaattagcc ataaagaaaa actggcgctg gagaaagata
     3001 ttctctggag cgtcgggcgc gcgataattc agctgattat tgtcggctat gtgctgaagt
     3061 atattttcag cgtggatgat gccagcctga cattattgat ggtgttattt atctgcttta
     3121 atgcggcgtg gaacgcgcaa aaacgcagta aatatattgc taaagctttt atctcatcgt
     3181 ttattgccat tacggtcggg gcgggaatta ccctggcggt gctgattctc tcagggtcga
     3241 ttgaatttat cccgatgcag gtgatcccta tcgccgggat gattgccggt aacgccatgg
     3301 tagcggtggg gttgtgttac aacaatttag ggcaacgggt cattagcgaa cagcaacaga
     3361 ttcaggagaa actgagtctt ggcgcgacgc cgaagcaggc ttcagcgata ttgattcgcg
     3421 acagtattcg cgcggcttta attccgacgg tcgattcagc aaaaacggtt ggcttagtga
     3481 gtttaccagg aatgatgtcc gggctgatat ttgccgggat tgatccggtg aaggcgatta
     3541 aatatcagat tatggtgacc tttatgctgc tctcaaccgc cagcttgtcg accattattg
     3601 cctgctattt aacctatcgt aagttttata attcgcgcca ccagttggtg gtgacgcaat
     3661 tgaagaagaa atgatgcgat gccggatgcg cagcatcgca tccggcgttg tggttcatgt
     3721 gccgatcaac ggaatatcaa tacaacaatg catacagctg gcggcgatac ttcgacgcca
     3781 gtgcatcacc cgtacccagc gcagcgagga tctcctggaa cgttttacgc gtctgaccgt
     3841 ctgcggcggt gagatcttta cgcagatgcc cgaacagcaa ctccagtgcc tcttcattgc
     3901 gcccaacctg atgcagttgc agcgccagtt gcgtcgccag tgcggcatct tctggattct
     3961 ccgccacctg ctgttgcaac tgttgaattt ccggcgtatc agccgcctgc ttcagcagtt
     4021 cgatttgcgc caccagcccc tggtagcggg tgtcctgatc ctgcaacgga atggttttca
     4081 gcaccgcttc cgcatcttca gaacggttca gcgcaatcag cgtttctgcc agcagcaggc
     4141 cgatctcccc gttctgattc gacaactgcc aggcgtcttt cagcaatggc agggcatcgg
     4201 tgtaattgcc ttcctgcatc agttgcatcg cctgctgcgc tttcagctct tcttcgcgcg
     4261 gcagcacttt atccagcagg gcgcggatcg cctcttccgg ttgcggcccc tggaagccgt
     4321 ctaccggttg cccgttctgg aacagataca cggtcggaat cgcacgcaga ccaaactgcg
     4381 cggcaatcat ctgctccgcg tcgcagtcca gcttcgccag aataaattgc ccgttgtact
     4441 gcgccgcgag gctttccaga attggggtta actgcaaaca gtgctggcta cgttcagacc
     4501 aaaaatagaa cagcaccgga gtggtcatcg actgttcaag aacctgttgc aggttagatt
     4561 cgttaatgtt gacaatattt tctacggaca tggagtcgct ctctgttgtc gatattttct
     4621 ttgacatggg ggcttaagcg cgcgcttcaa ctcacccctg caatattttg tccatcacgc
     4681 gccccggcag caggcgctta agcaccatta ccgcccaggt caccagcgtc accggatagc
     4741 gcatcttcgg cttctcgcta ataaaagcat ggcgtacttt gtccaccacc gcttccggtc
     4801 ccaacgtaaa gcgggcggcg atgccgggat tttcgactgg tttatcactt tgcgtctggt
     4861 tgacgttgtc ggtgaagcga gtacgaatgg gaccgggttc gatcaggctg actttaattc
     4921 cgctgtggcg tagctccatg cgcagcgcgt ctgaccacgc ctccagcgca tatttactgg
     4981 ccgcgtaagc gccacgaccc ggcgtggaga ttaatcccat caccgatgat gtcatcacaa
     5041 tacgcccttc accgtgcggt aacatcgcgg gtaacaggcg catggtgagc tggtgtgcgc
     5101 cgaaaaagtt ggcggaaaac tgctgttcca tctgcgcacg gctgatggtg gaaagggggc
     5161 catacatgcc gaatccggca ttgttaaaga tcccatacag acaattatcg gtcagggcga
     5221 tcacctcgtc ggctgcgcga tcaacacttt ctggtgaatc cagatcgatc aacacgccgg
     5281 taaatcccat gctgttcatg cgctcaacat catccggttt ccggcaacct gccagcacat
     5341 gaaaaccctg gcgttttaat tcgagcgcgc tttccaggcc aattccactg gaacatccgg
     5401 taattaagac cgatttttgc ataactttac ctgtcaggat ctccgttgct ttatgagtca
     5461 tgatttacta aaggctgcaa ctgcttcgcc atccagtcgg caataaacgg ctgggcgtcg
     5521 cggttgggat gaataccgtc atcctgcatc cattgtggct tcaggtagac ctcttccata
     5581 aaaaagggca gcagcggaac atcaaactct ttggcgagtt tggggtaaat ggcgctaaag
     5641 gcttcattat aacggcgacc atagtttgca ggcagacgta tttgcattaa caatggttca
     5701 gcgttggcgg ctttgacatc ctgcaaaatc tggcgcagcg tttgctcggt ttgctgtggc
     5761 tgaaaaccac gcaaaccgtc attgccgccc agttcaacca gcacccagcg cggctgatgc
     5821 tgtttcagca gagccggaag gcgcgccagc ccttgttgcg aggtgtcgcc gctgatgctg
     5881 gcattaacta ccgacgtttt actctgccac ttatcattca acaaggcagg ccaggccgcg
     5941 ctggcagaca ttcgataccc ggcgctcagg ctatcaccca gaatcaataa cgtgtccgct
     6001 gcggcggcac ggaaggttaa caggaccagg aacaggaagg gcaaatgcca gcggaaaaca
     6061 ttgttgaagt tcatcatctt aagaagtccg tcggtcaggg ggagcatgaa ctctccatcc
     6121 tcaccggagt tgagttggtt gtcaaacgcg gcgagaccat cgcgctggtg ggcgagtcgg
     6181 gatcgggtaa gtcaaccttg ctggcgatcc tcgccgggct ggatgacggc agcagtggcg
     6241 aagtgagttt ggtgggacaa ccgctacata atatggacga agaagcgcgg gcaaagttgc
     6301 gcgcgaagca cgtcggcttt gtttttcagt catttatgtt aattcctacc cttaacgcgc
     6361 tggaaaacgt cgagcttccg gcgctgctgc gcggtgagag tagcgcggaa agtcgtaacg
     6421 gagcgaaagc gttgctcgaa cagttagggc tgggtaaacg tctggatcat cttccggcac
     6481 agctttccgg cggtgaacag caacgagtgg cgctggcacg agcctttaac ggtcgacctg
     6541 atgtgctgtt tgccgacgaa cccaccggca accttgaccg ccagaccggc gataaaattg
     6601 ccgacctgct gttttccctc aaccgtgaac atggcaccac gttgattatg gtgacccacg
     6661 acctgcaact ggcggcacgc tgcgaccgct gcttacggct ggtgaacggg cagttgcagg
     6721 aggaagcatg attgcacgtt ggttctggcg cgaatggcgt tcgccgtcgc tattaattgt
     6781 ctggctggcg ctaagcctgg cggtggcctg cgtgctggcg ctgggcaata tcagcgatcg
     6841 catggagaag ggcttaagcc aacaaagccg ggagtttatg gcgggcgatc gggcgttgcg
     6901 cagttcgcgc gaagtgccgc aagcatggct ggaggaagcg caaaagcgcg gcctgaaagt
     6961 cggcaagcag ctgactttcg ccaccatgac atttgcaggc gacacaccgc agctggcgaa
     7021 cgtcaaagcg gtggatgata tctacccgat gtatggcgat ctgcaaacca atccccctgg
     7081 cctgaaaccg caggcgggca gcgtattgct ggccccacgc ctgatggcac tgcttaacct
     7141 gaaaacgggc gacaccattg acgtgggcga tgccactttg cggattgccg gagaagtgat
     7201 tcaggaaccg gattccggtt ttaacccctt ccagatggct ccgcgtctga tgatgaatct
     7261 ggcggatgtc gataaaaccg gagccgtgca gccggggagt cgggtcaact ggcgctataa
     7321 attcggcggc aacgagaacc aactcgacgg ctatgagaaa tggttgttac ctcagcttaa
     7381 acccgaacaa cgctggtacg gtctggaaca ggacgaaggc gcgctggggc gatcgatgga
     7441 acgctcgcaa cagttcctgc tgctttcggc gcttctgacc ttgctgctgg cagtggcagc
     7501 agtggcggta gcgatgaatc attactgtcg cagtcgctac gatctggtgg cgatcctcaa
     7561 aacgctgggg gcagggcgtg cgcaactgcg taagctaatc gtcggtcagt ggttgatggt
     7621 gctggcgctt tcagccgtta ccggtggggc gataggcctg ttgttcgaaa acgtgttgat
     7681 ggtgctgctc aagcccgttc tgcctgctgc actaccgcct gccagcctct ggccgtggct
     7741 gtgggcgctt ggcaccatga cagtcatctc gctgctggtg gggctacgac cataccgctt
     7801 gttgctggca acgcagcctt tacgcgtatt acgtaatgat gtggttgcga acgtctggcc
     7861 gctgaagttt tatctgccga ttgtcagtgt ggtggttgtg ctgctgctcg ccggattaat
     7921 gggtggcagc atgctgcttt gggcggtgct ggcgggcgcg gtagtactgg ctttgctgtg
     7981 cggtgtgctg ggctggatgc tgctgaatgt acttcgccgc atgacgctga aatcgctgcc
     8041 tctgcgcctg gcggttagcc gcctgttacg tcagccgtgg tcaacgttaa gccagctttc
     8101 ggcattttcg ctctccttta tgctgctggc actgctgctg gtgttgcgtg gcgatctgct
     8161 cgaccgctgg cagcagcagc tacctccaga aagcccgaac tactttttaa tcaacatcgc
     8221 cacagaacag gtagcaccgc taaaagcgtt cctcgcggaa catcagatag tcccggaatc
     8281 gttttatccg gtggtgcggg cgagactgac ggcgattaac ggcaagccga cagaaggcaa
     8341 tgaagatgag gcgctcaatc gcgaactcaa tcttacctgg caaaatacgc ggcccgatca
     8401 taatccgatt gtcgccggta actggccgcc aaaagccgat gaagtgtcga tggaagaggg
     8461 gctggcgaaa cgcttaaacg ttgccctcgg tgataccgtg acttttatgg gcgataccca
     8521 ggagttccgc gctaaagtga ccagcctgcg caaagtggac tgggaaagtc tgcggcctaa
     8581 tttctatttt attttccctg aaggggcatt agacgggcaa ccgcagagct ggcttaccag
     8641 tttccgctgg gagaatggca acggcatgtt aacgcaactc aaccgccagt tccctaccat
     8701 tagcctgtta gacattggcg cgattttaaa acaggtcggt caggtgctgg agcaggtaag
     8761 tcgggcgctg gaggtgatgg tggtgctggt caccgcctgc ggtatgttgc tgttgctggc
     8821 acaggtgcag gtgggaatgc gtcagcgtca tcaggagctg gtggtgtggc gcacactcgg
     8881 tgcggggaaa aaactgctgc gtaccacgtt gtggtgtgag ttcgccatgc ttgggtttgt
     8941 ttccggcctg gtggccgcaa ttggtgcgga aacggcactg gcagtgttgc aggcgaaagt
     9001 gtttgatttt ccgtgggagc cagactggcg attgtggatt gttctgccgt gcagcggagc
     9061 gctgctgctg tcgcttttcg gcggctggct gggtgcgaga ctggttaagg gtaaggcgct
     9121 gttcaggcag tttgcggggt gatgagagta aataaggggc gttctggttg aatggaacgc
     9181 cttgtttaaa ttaattctac aatatgtttc attgatgatg ttattgaatt gg
//
LOCUS       DAZUCE010000197         9186 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZUCE010000197 DAZUCE010000000
VERSION     DAZUCE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.197"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..449)
                     /locus_tag="ACHY03_003991"
     CDS             complement(<1..449)
                     /locus_tag="ACHY03_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS3907169.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSNETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSP"
     gene            complement(446..2272)
                     /gene="atoS"
                     /locus_tag="ACHY03_003992"
     CDS             complement(446..2272)
                     /gene="atoS"
                     /locus_tag="ACHY03_003992"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFS3907170.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTVRKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELNPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            2439..5288
                     /gene="rcsC"
                     /locus_tag="ACHY03_003993"
     CDS             2439..5288
                     /gene="rcsC"
                     /locus_tag="ACHY03_003993"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFS3907171.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLENSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(5488..6138)
                     /gene="rcsB"
                     /locus_tag="ACHY03_003994"
     CDS             complement(5488..6138)
                     /gene="rcsB"
                     /locus_tag="ACHY03_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFS3907172.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(6155..8827)
                     /gene="rcsD"
                     /locus_tag="ACHY03_003995"
     CDS             complement(6155..8827)
                     /gene="rcsD"
                     /locus_tag="ACHY03_003995"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFS3907173.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2243 a         2152 c         2351 g         2440 t
ORIGIN      
        1 gggctgttgg tgagaatgtg cccccattgc cagctggtgc tcagtgcctg gtgcagatga
       61 cggatctctt ttttcattga ttggagttgt aaagcgcgct gaacgattaa attcaactca
      121 tcgagatcaa acggtttaat aacatagtcg aaggccccgc agcgtagcgc ttcgacggcg
      181 gtttccactt cagcataggc cgtcatcaga ataacgggtg tccgggtctc attactgcgc
      241 atctccttta gtgccttgat gccgtccatc tctggcatgc ggatatccat caacaccacg
      301 tcagggtgaa tatcagcaaa caggtgtaat gctgtgcgtc cgttgttcgc acaatgtgtt
      361 tcgaaacctt gtagtgcaaa agcggtgctc agcatacggc gaacattatc ttcatcatcc
      421 acaataagga tgcgattaat agcagtcata cagtctgatt tccctgcggg ttgattggta
      481 aaataagcgt gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat
      541 gggcattaat gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg
      601 tgaaaaaggg atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct
      661 ctatcgaaat ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc
      721 gtgcgctgat agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt
      781 cacgatcggc gttaatcggg tttaattcat tatccagttc gcttatgaag tcgacccgcg
      841 cttgtacgcc ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgagactga
      901 cttgttgcca ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt
      961 taattgaatc tatttctttg agtactacgg acagatattc ctgatgtatt aggtcgctgg
     1021 tttgttggcg caagatctgt acataaccac gaatagccgt tagcggatta cgtacttcat
     1081 gcgcgacgcc agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc
     1141 gctgggtttc tttgcggaca gttaaatcag agaaaatcac caaagcacct atcatttcac
     1201 cgtgcgtgtt atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacggcctg
     1261 gaaaactgat ctccagcgcc acatgttcgg tgccatgttc cagtgtatcc agtactggac
     1321 tgtagaactg agtattgtcg aacaacatgg aataaggctg ccctaccagt tcatggcgtt
     1381 gatagccagt gataacttct gctgccgggt tcatggtggt tacatcaccc tggcggtcaa
     1441 tggcaatgac gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac
     1501 gcagtgcttg ggcgaggttg ttaacactct gactgatttg ccccatttca ccgggcaatt
     1561 gtggtaatcg agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa
     1621 tattggcgct cagacgacgg gagaaaagga caatcaacag caggcttatc agcaaaccgg
     1681 cggtgagcac aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg
     1741 ttaattcatt ggcccagata tagccgagga tttcaccatt acgctcaatg gggatcattg
     1801 aattcaaaat atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca
     1861 tgacttcgcg accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg
     1921 ctgaaggcgc gtaggtgatt atcgcatcca gcgttttgtt gtaataacca gcgccgatgc
     1981 cagggaaggc gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga
     2041 tacgctcctc acgtggtaag tcgatgtaga gatcatagcg atcgcctagc gcctgattaa
     2101 gcaggttgac cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt
     2161 ctgtttctac gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca
     2221 gaatcatttg attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat
     2281 ccactagtct tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg
     2341 tgtatcatat tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac
     2401 cacactccat cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc
     2461 gtacaaccct gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt
     2521 tgattgcttt ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa
     2581 ttcgtcagga atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg
     2641 atgtgatgaa agagctgaag tacatcgctg aaaatcgctt atcggcagaa aacggtgtgc
     2701 tttccccgcg tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg
     2761 actctgattg ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt
     2821 ttatgcgcta ctggcgcgat aatttttctg cggcttacga tctcaaccgg gtatttttaa
     2881 tcggcagcga taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg
     2941 ataccgcgtt gaaagcgttg catgaacgca tcaataaata tcgaaatgca ccacaagatg
     3001 atagcggcag taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt
     3061 acgcgttgac gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga
     3121 ccatccggat ggagaatttt ttcttacctg gtacgttgcc gatgggggtt accattcttg
     3181 atgaaaatgg tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc
     3241 ctcgctggat gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc
     3301 tgaagaaaaa tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg
     3361 tgctggaacg cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag
     3421 cagcattgtt tactctcgcg cggatgtacg agcgacgtat tttcattccg gcggaaagcg
     3481 acgccctgcg actggaagaa catgagcagt ttaatcgcaa gattgtcgcc tccgcgccag
     3541 tgggtatctg cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc
     3601 atacctatct caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg
     3661 ggcagcaggt caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct
     3721 tcgtccattc gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt
     3781 cgcgcgtgaa gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc
     3841 agtcaaaatc gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca
     3901 ttatcggtaa cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcgtctgg
     3961 tgacggcaat gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt
     4021 tctcgaagat tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag
     4081 tgatgaacca catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt
     4141 actgctttat tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc
     4201 aggtcatctc caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt
     4261 tgcatgtccg cgctgatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga
     4321 ttccggcgaa agaagtggtg cgcttatttg atcccttctt ccaggtcgga acgggcgtac
     4381 agcgaaactt ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga
     4441 tggacggcga tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta
     4501 ttccgttgta cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac
     4561 gctgctggct ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaaac agtttgcagc
     4621 gcagcggcat cgtcgtaaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa
     4681 tcactgacga ggtagtgagt aaaaaatggc agggcagagc ggtagtgacc ttctgtcgtc
     4741 gccatattgg tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgcac
     4801 cgcatgagct accggcattg ttggcgcgta tttatttgat cgagatggag agcgatgatc
     4861 ctgctaacgc tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc
     4921 tggtcgtgga tgatcatccg attaaccggc gtttgctggc agatcagttg ggatcgttgg
     4981 gctatcaatg taaaaccgcg aatgatggcg tcgatgcgct taatgtactt agcaagaatc
     5041 atattgatat tgtgcttagc gacgtcaaca tgcccaatat ggatggttac cgcttgacgc
     5101 aacgcattcg tcagttggga ctgacgttgc cggtaatcgg agtaacagct aatgcgttgg
     5161 ctgaagagaa gcagcggtgt ctggagtccg gtatggacag ctgtctgtcg aagccagtaa
     5221 cgctggatgt gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg
     5281 aatcgtagga cggataaggt gtttatgccg catccggcaa tcggtgcaaa tgccagataa
     5341 gacactaacg cgtcttatct ggcctacttt aatgtaatct gttgaaataa tgggaatcgt
     5401 aggccggata aggcgtttac gccgcatccg gcaatcggtg caaatgccag ataagacact
     5461 aacgcgtctt atctggccta caggtgatta gtctttatct gccggactta aggtcacaga
     5521 agagagataa ttcagcaggg cgatatcgtt ctcgacaccc agcttcatca tcgcagattt
     5581 cttctggcta ctgatggttt taatactgcg gttcagcttt ttagcgatct cggtcaccag
     5641 gaagccttcc gcaaacaggc gcagaacttc actctctttt ggcgagagac gcttgtcacc
     5701 gtaaccacca gcactgattt tttccaacag gcgagaaacg ctttccgggg taaatttctt
     5761 ccctttctgc agcgcggcga gagctttcgg cagatcggtc ggtgcacctt gtttcagcac
     5821 gatcccttcg atatccagat ccaataccgc actaagaatc gccgggttgt tgttcatggt
     5881 cagaacaata atcgacaggc ttgggaaatg gcgcttgatg tacttgatta aggtaatgcc
     5941 atcgccgtac ttatcgccag gcatggagag atcggtaatc aacacatgcg catccagttt
     6001 cggcaggttg ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat tcacccactc
     6061 aatttgctca agtgatttgc gaataccgaa caagactatc ggatggtcat cggcaataat
     6121 tacgttcata ttgttcatgt aataggctac cttgctacag caagctcttg acataactgt
     6181 caatgtcgct gatgtatttt tctattcctg gaacatcctt ctcacgaatc agatgttcca
     6241 gcgtttcaca taactgcttg ccgggtacca gatttagcat ggcaaatacg cctttaagac
     6301 gatgagccgt ttgggctaac gcagcaaagt cactggttgc tgcttcagta tacagcctct
     6361 taacatcatc cggtactgtg tctacaaaga gcgcataata gccgctggca tggagttgcg
     6421 cattttcatc tccgcccaga ggcgattctg tcacctcttc ctgcgccagt tgcacttcaa
     6481 ttaattgtaa gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg cacaattgac
     6541 caggcccaat ttcccgtacg ccagactcat catcgcttaa aagcaagcca gaggcagtaa
     6601 gattagacgg attatccgtt aaaaagatat cataatcttg actaattaat ctttcatcgg
     6661 gtgtgataca ggttgcaccc caattttcta actggcgagt gacaatattc cgaatttctg
     6721 ccgaagtaac atccaccatt acgcagacat catccagtaa acgctcttct tcctcttcaa
     6781 cttccgggtc agctgcgagc attttgatat gcacagagta gcgtgtacca agcccatccc
     6841 gcgttttgat gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc agccagaatg
     6901 ccagcgggtc cgccttgcca tagcgatcgt tttgggtctg gttgataaac gggaagtgca
     6961 aattatccat ttcatgaatg cttacgcctt ctccggtgtc cagaatgcgg aacgtcaggc
     7021 ggtcttcgga ggactcatcc tgatcaacct caagggtgat ttttcccaat tgcgttgagg
     7081 tcacggcata ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga tcgccgcggc
     7141 gcatatcgtg tgctttcaga tgattgttaa tcagcagttg cagaccttta cgcttgatgg
     7201 caggcaacac tgaaggcaca acttcatcaa ttaaatcctg cacggagaac agcaccgtct
     7261 cacttttcca gctatcgtcc gcaagcatgt tcgctaactg tatttcatcg accagccgca
     7321 ccagcacatc tgcctgattc gccagttgtt tgctttccgg ggcgttgagt ttagccgcgc
     7381 tctccgccag ggactgtgca ggttctttca gcgcatcgcc aatgtttttc ataaaggtca
     7441 tccgcccctg ctggtttttc tcatacagac gctgcgcctg cttgagtttc ttgtttacca
     7501 gcacttcccg atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg acctggctgc
     7561 ggaacatgcg gatctcatac agctcgttat tgatcgtcgc ctgaataatc ccctgatgct
     7621 gttccgccat ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga tcggcaattt
     7681 tgttacttat gactgtgcgg ttcgattcct gatcgtgaac caacaggccg agcggcagca
     7741 gtgagactat ctcttcattg attgcccgta aaatgcgcaa ttcgttattg accgccgtgt
     7801 tgggtagact ttctgtactg cgactggaga aatggcggaa tgtggtatag ccaaataacg
     7861 ccagcgccag caaaccgatg ttcagcagca gtggtagcag aatgttttgc aacgtatcca
     7921 gcaataaggt gccataagga acctgccaga ccagtcgcat atcggtagag ttgagtgccg
     7981 aggagatttc aatcttcgta ctgttaaagt ggatactgac gctatccgtc ccttctttct
     8041 cattatcatt gtctcccgtt gccgtcgcgt caggctcaag gcggaaactg tccagcggca
     8101 tacccggtgg gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt gccagatgtc
     8161 ccggctggtt aaaggtggta cgcaaggtaa agtaatgacc gttctgccag gccaggcggc
     8221 gcatgttaga aaagctttcg cgttcatcga gggcgttggc ctgttgcaac atctctgcac
     8281 gacgtgaatc aacaatgtcg ctgacggtcg attctttaaa tccggaggtg agatctttga
     8341 ggggcagggt tgagatcagc accagactgt tatcctgacc attcaggtaa tacatcgacc
     8401 acggtacatt ttctgcaccc cacaatgtat ccagataagt ggacatccgc tgagtcatct
     8461 caagcgttga gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg cgtggtttct
     8521 ccagatagta gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg gaggaggggg
     8581 aggtcgtcgc ggcaatgttg tcgtagatct gccaggtcac gtaacgccag gtatcgacgc
     8641 gcttttggat tgcgtgggta atgtcgacaa tctggtaact tttatctttc agccaggcgt
     8701 taacggcgct ttgtaccatt acacccatcg tcaccagtaa cacaatgatc aacagtaaaa
     8761 agaagcgggt aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt gtctctttct
     8821 gacgcattcg tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag tataaagggt
     8881 acagtgtgaa attccagact cttacctgat tgatgattct ctattctccc gtgatattta
     8941 tcatcctgca ttgggggaaa tagatgtaca agcgccattt tgctgtacaa ataacctacg
     9001 aaaagcccaa cgaaattaat tgcaaataaa cgaagattgc tggaaattat gctgatgtta
     9061 attatttgtg aaatagttaa caagcgttat agtttttctg tggtagcaca gaataatgaa
     9121 aagtgtgtaa agaagggtaa aaaaaaccgg atgcgaggca tccggttgaa ataggggtaa
     9181 acagac
//
LOCUS       DAZUCE010000198         9175 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZUCE010000198 DAZUCE010000000
VERSION     DAZUCE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.198"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(27..929)
                     /gene="yihR"
                     /locus_tag="ACHY03_003996"
     CDS             complement(27..929)
                     /gene="yihR"
                     /locus_tag="ACHY03_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFS3907174.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAVHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            complement(996..2237)
                     /gene="yihS"
                     /locus_tag="ACHY03_003997"
     CDS             complement(996..2237)
                     /gene="yihS"
                     /locus_tag="ACHY03_003997"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFS3907175.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            complement(2253..3131)
                     /gene="yihT"
                     /locus_tag="ACHY03_003998"
     CDS             complement(2253..3131)
                     /gene="yihT"
                     /locus_tag="ACHY03_003998"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFS3907176.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(3155..4051)
                     /gene="yihU"
                     /locus_tag="ACHY03_003999"
     CDS             complement(3155..4051)
                     /gene="yihU"
                     /locus_tag="ACHY03_003999"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFS3907177.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            4219..5115
                     /gene="yihV"
                     /locus_tag="ACHY03_004000"
     CDS             4219..5115
                     /gene="yihV"
                     /locus_tag="ACHY03_004000"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFS3907178.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDYTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAT
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5149..5934
                     /gene="csqR"
                     /locus_tag="ACHY03_004001"
     CDS             5149..5934
                     /gene="csqR"
                     /locus_tag="ACHY03_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFS3907179.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNSRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            6033..6632
                     /gene="yihX"
                     /locus_tag="ACHY03_004002"
     CDS             6033..6632
                     /gene="yihX"
                     /locus_tag="ACHY03_004002"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFS3907180.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            6626..7498
                     /gene="yihY"
                     /locus_tag="ACHY03_004003"
     CDS             6626..7498
                     /gene="yihY"
                     /locus_tag="ACHY03_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFS3907181.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            7495..7932
                     /gene="dtd"
                     /locus_tag="ACHY03_004004"
     CDS             7495..7932
                     /gene="dtd"
                     /locus_tag="ACHY03_004004"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFS3907182.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            7977..8918
                     /gene="fabY"
                     /locus_tag="ACHY03_004005"
                     /gene_synonym="yiiD"
     CDS             7977..8918
                     /gene="fabY"
                     /locus_tag="ACHY03_004005"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFS3907183.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGYETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2133 a         2306 c         2455 g         2281 t
ORIGIN      
        1 atgatctcgt tcttttctta ataaaattaa ttgtgttgcc cgtaaatatt aaaaaacagg
       61 cggtgcgttt tccctggttc cagtaataat aagtcgagac ctgaattaaa ggcgttaggc
      121 gggcaactca taggttcaac tgccagtccc tgacgctgta atttttcgcc gctataaatt
      181 tgtacccagg gttgatcgga acaaaggcta accgaaagcg cctgttgtgg atgggtaatg
      241 gttatttccc acagatcatt agcggtttta aaggtgtggt cgatttttgt cgcggctatt
      301 tttttcgcct gcgtgaaatt taaatccagc tcatcaacgt ggtgaagcgt tgtcggatta
      361 gcgtgctcat cgacggcata aacctggttt gccggaagct ggaacagata ttcatcaaca
      421 gatgttaagt tgcaggttaa ataaggatga atacccacac catagggtgc ggtcacagta
      481 ccaatattct gacttgcgat ctcaacggaa agacccgtgc gtgcattgag cgaataaaca
      541 acctgcgaag ccagcataaa cggatagccg taactgggcg gcagaaaagc tgtcagggta
      601 acgctggtgg cggttaattc gctgatttgc cagtcacgcc aggcgagcag cccatgaaca
      661 gcggcttttg aaccatgttc attaatgggt aactgatatt cctgcccctg atagcgataa
      721 cagccgttgg cgatccggtt cggccacgga ataaggactt tgcccagatg tgccagcggc
      781 atctcttcgg gtttatgtgg aataactaaa tgacagccct gaaaggttaa ttcagccaga
      841 cctgcaccga cggtcacaat ggtcgcgtga taatcccccg cggcaagtga aacggtctgt
      901 ccactgcaat gcatgttggt gatttgcata cgaggtacct ttattgaata acgacatacc
      961 cggcgtgcag cctgatatgt cgttttttta gtcagttatt tcgcgttaat atccagcaga
     1021 cccgccgcaa ctgccggagc caggcctggt gctaacggga tacgcgggat caccaggcaa
     1081 tgcagcaggt gataaatatc ctgtttgccg tcccagactt tggtggtgac cttattgtcc
     1141 gcatccagct cctgccacca tgaaccattt tcgtagtcca tcaggtattt gatgcagtag
     1201 tcccaccatg tctgatacca ggtttcgtac tggcgatcgc cggtgacggt gtagagcgcg
     1261 taggccgtac ccatcgcttc gacgataggc caacgcacac gttcgcggac caccggtttt
     1321 ccttcccagt caacggtata aacaatcccg tccgcaccat cgggtgccca ggcatcacgc
     1381 acggtggcgt taaacagacc tttggcatct tctagcagcc atgctggcgg ttgttcgcaa
     1441 cgggcttcca gggcagcatg gatgtgcagc attaaacggc cccattcgat ccagtggcct
     1501 ggtgtacctc cgaacgcgcg gaagcggtgc gccgggttat ctttgttgta atccggcagc
     1561 ggattccact gggtatcgaa gtgttcgtta acgcgataat gattatttct tgcgacgtcg
     1621 tggataatca cggaagccac gcgaatcgcg cgatccagcc attttttgtc atgcgttacg
     1681 tcataaacaa ttaagaaagc ttccactgcg tgcatattgg cattgccgcc gcggtactct
     1741 tcggttttgc tgaaggcttc gtcccaggac tccaggcaca tctgctcttc ttcgctccag
     1801 aagtatttct cgataatttc aatggtgtaa tcgagcagct tgcgcgcttc cggatgaccg
     1861 gtagtgacgg cgctggcagc acccagcaga gcaaagaaat gttgatagcc ctgtttggag
     1921 gcatccacca cgccctcgtc attcacacag gcataccagc cgccatattt tttatcgcgc
     1981 agtgcgccgt tcatggcttt aataccgtga tcaaccaacg agtaagcgcc aggtcgaccc
     2041 atcgccgcag caacggaata aacgtgcaac atacgagctg tgatccacag atgggtgccc
     2101 atctcttctt tgatttgccc tttattgcct aaccagccaa atccggtcgg tactacggaa
     2161 tttttaccaa aatcaaagat gcgatcggtt tcctgttcca gccaacggtt gtgacttaaa
     2221 gtgttaaacc atttcatttt tgtatcccca ttttaacggc gtttagccat catttcgtcg
     2281 acgatatcgc caagttgttg taatttcggt gcgcaaacgt cacgcagcat cagctcgtta
     2341 tctggtaaac cgacgaccga tgcccagacg gcacgaccag ccaggaagcc cgatgcaccc
     2401 gctgtcattg ccacgcgtac ggcacgcggg aacagttttt cgtcgacacc ggaagagagg
     2461 atcacccacg gcatattgat atggtcattc agacgttgtg aagcactgag aagctcttgt
     2521 tgcggacctt taccgtaaag cggcatttca actttgtaga ggtcagcgcc actgtcgccc
     2581 agctctttgg cggcatcgat gatcgcttgt tcgcgatcga atttatcgcc acgacgtggt
     2641 gggcggacga ctggctcaat aatgcttacc aggccgtgtg agtggcacag ttcgttaaac
     2701 tctttcacca tatccaggcg ttgctgcgca tcttcatcac tacgccacag caccagcagt
     2761 tttaaggctt tgccaccgtc ttgtttgatc tgtagcggat tgattttgcg gtcaatgacc
     2821 acgctatcta ccggaatacc gttgccagga atgaactcat cggcggcgac aatcatggcg
     2881 caacttttgg caatcgcgtt ttgctcaacc acctggcggt aacagaattg ttgatctacc
     2941 agaatcgccg aggcataagg cgagagggtc ttcgccgcgt taactttgaa atcagttaaa
     3001 acgctatcgg ctaccggagc gggtgcccca gccgcggcaa acatcatgcg catggcttcg
     3061 cgctgatcga ccgccagcat ggcaaaaccg cccgaggcgc gcgtaatgtc gttgatggtg
     3121 tacttattca ttcatttatt cctttatcca gtcgttacat ttttactttg gcagtcatcc
     3181 cggcactgac acggacctgt tccagaatgg cggaccagtc ctggcgaccg cgacccgctg
     3241 cgcgcgcctg gctataaacc tcccgtgagg cggcccccag cggcattggc acatgcagct
     3301 ggttggcgac atcaagggcg atgccaagat ccttatgggc aagatcgatc atgaaggcgg
     3361 gagaaagatc tccgctgagg actttgtttg gccaggaagt ggtgaagtgg cctttaccgg
     3421 cggcggtacc gctcatcact ttgacggcaa catcgaaggg aagattcagg gcttcgcaca
     3481 aaacggcagc ttctgccgaa agcgcattga gtgcgatgct catatagttg ttgatgagct
     3541 taacgcggat ccccatgccc ggaccgccag cgttgatcaa ctcactgccc atcgccatca
     3601 ggatcggcgt ggcacgttca acttgttcag cggtgccgcc agccagcagt aacagagtac
     3661 cggtaatggc atttgcagaa gtacggccta ccggaacatc catcatgctg aagcctttgg
     3721 cttgcatatc ggcaatcaat ttatcggttt gcagcggatg gatggtcgac atatcaatga
     3781 ccagcgcatc ggtagataag ctttcgcaaa cgccgttttc accgaacaac acgctgcgca
     3841 ccagatcgcc attcggcaac atggtaatga taaattcggc atcttttgcg gcctgcgccg
     3901 gattggcggc gggagtcgcg cctttgtcta ccagatgccg cacagcctcg gcattcacat
     3961 caaagacgcg aagttggtgc ccttgctgca ataaattgct cgccattggc gaacccattt
     4021 gtcctaaacc gataaacgcg attgctgcca taaccttctc ctgaatacag tgatgtcact
     4081 ttttgtcatt tatgacacgc tttgattgtc tgtttttgat cgtatttgta atttatagtc
     4141 aaaaaattga cagccatcac tttttaaaca attggtgaaa ttaaaatgaa cgcatcccaa
     4201 aatgtttaag gaatgaccat gattcgtgtt gcttgtgtag gtataaccgt gatggatcgc
     4261 atctattacg tggaagggtt accgacggag agcggtaaat acgtggcgag aaattatacg
     4321 gaagttggtg gcgggccagc ggcgacggca gcggttgcgg cggcaaggct gggggcgcag
     4381 gttgatttta ttggtcgcgt aggtgatgac tacacgggca acagcctgct ggcagagctg
     4441 gaatcctggg gcgttaacac ccgttacacc aaacggtata accaggcgaa atcatcgcaa
     4501 tccgccatca tggtggatgc cacaggcgag cggataatca ttaactaccc cagcccggac
     4561 ctgctgcctg acgcagagtg gttggaggaa attgatttct ctcagtggga tgttgtgctg
     4621 gcagatgtac gctggcacga cggtgctaaa aaagccttca ctctggcccg gcaggcgggc
     4681 gtgatgaccg ttctggacgg tgatattacg ccgcaggata tcagcgagct ggtggcatta
     4741 agcgatcatg cggccttttc agaaccgggt ctggcgcgct taacgggtgt gaaagagatg
     4801 gccagtgcgc taaaacaggc acaaacgctc acaaatggtc atgtctatgt gacccagggc
     4861 agcgcaggct gtgactggct ggaaaatggc gggcgtcaac atcaaccggc cttcaaagtt
     4921 gatgtggtag ataccacagg tgcgggtgat gtttttcacg gcgcattggc ggtggcgctg
     4981 gcaacaggtg gggatttagc ggagtcagtc cgcttcgcca gcggtgtagc ggcgttgaaa
     5041 tgcacgcgtc ccggtggacg agccgggatc cctgactgtg atcaaacccg atcttttttg
     5101 tcactttttg tataaaatgc cagggtgatg gtttttcgag gaattttcat gagccttacc
     5161 gaactaaccg gtaacccgcg gcacgaccaa ctcctcatgc tgatcgccga gcgtgggtat
     5221 atgaatattg atgagctggc aaatctgctg gatgtctcca cgcagacggt gcgccgggat
     5281 attcgtaaat taagcgagca aggcctgatt acgcgccatc acggtggcgc gggtcgggct
     5341 tccagcgtcg ttaatacggc gttcgagcag cgtgaggttt cgcaaaccga ggaaaaaaaa
     5401 gcgattgccg aagcagtggc agactatatt cctgatggtt caacaatatt tatcaccatt
     5461 ggtacgaccg ttgagcatgt cgcccgggcg ttacttaacc ataatcattt gcggataatc
     5521 accaacagcc tgcgtgtggc gcatattctt taccacaact cgcgctttga agtgatggta
     5581 cccggcggta cgttgcgctc tcataatagc gggatcattg gcccttcagc ggcgtccttt
     5641 gtggccgatt ttcgtgctga ttatctggta acaagcgttg gggcgattga gagcgatggc
     5701 gcgttgatgg agtttgatgt aaacgaagct aacgtggtga aaacgatgat ggcgcacgcg
     5761 agaaatatcc tgctggtcgc cgatcacact aagtatcatg cttcggcggc ggttgaaatt
     5821 ggtaacgtgg cacaggtcac cgcgctcttt accgacgagc tgccgcccgc tgcgctaaaa
     5881 tcacgcttac aagacagcca aattgaaatt attcttcccc aggaagacgc gtagattttt
     5941 gtgaccgttg acctggcttc ataccttgcc acatagccaa acccatcctt tcccgctaca
     6001 gttaatttct tgtggcgcga aaggaggcaa aaatgctcta tatctttgat ttaggtaatg
     6061 tgattgtcga tatcgacttt aaccgtgtgc tgggagcctg gagcgattta acgcgtattc
     6121 cgctggcatc gcttaagaag agttttcata tgggggaggc gtttcatcag catgagcgtg
     6181 gggaaattag cgacgaagcg ttcgcagagg cgctgtgtca tgagatggct ctaccgctaa
     6241 gctacgagca gttctctcac ggctggcagg cggtgtttgt tgcgctgcgc ccggaagtga
     6301 tcgccatcat gcataaactg cgtgagcagg ggcatcgcgt ggtggtgctt tccaatacca
     6361 accgcctgca taccaccttc tggccggaag aatacccgga aattcgtgat gctgctgacc
     6421 atatctatct gtcgcaagat ctggggatgc gcaaacctga agctcgaatt taccagcatg
     6481 ttttgcaggc ggaaggtttt tcacccagcg atacggtctt tttcgacgat aacgccgata
     6541 atatagaagg agctaatcag ctgggcatta ccagtattct ggtgaaagat aaaaccacca
     6601 tcccggacta tttcgcgaag gtgttatgct aaaaaccatt caggacaaag ccaggcaccg
     6661 tacccgtcca ctatgggcct ggctaaaact actctggcaa cgcattgatg aggacaacat
     6721 gacaaccctg gcaggtaacc ttgcctatgt gtcgttactc tcattagtgc cgctggttgc
     6781 cgttgttttt gcgcttttcg ccgcttttcc catgttttcc gacgtcagca ttcagttacg
     6841 tcactttatt tttgccaact ttctgcctgc tactggcgat gttattcagc ggtatatcga
     6901 acaatttgtt gccaattcca acaagatgac cgccgttggc gcgtgcgggc tgatcgtcac
     6961 cgcgttattg ttgatgtact ccatcgatag cgcgttgaat accatctggc gcagtaaacg
     7021 agcgcgaccc aaaatttact cgtttgccgt gtactggatg attttaacgc tggggccgct
     7081 gctggcaggg gccagtctgg cgatcagttc ctatttgctc tccctgcgct gggcgagcga
     7141 tctcaatact gtcatcgaca acgtgctgcg tatttttccg ctgctgttgt cgtggatctc
     7201 cttctggttg ctgtacagca ttgttcctac catccgcgta cctaaccgcg acgcgattgt
     7261 cggcgcgttt gtcgctgcac tcctgttcga agcaggaaag aaaggtttcg cgctttatat
     7321 caccatgttc ccgtcatatc agctcattta cggcgtgctg gcggtgatcc ccattctctt
     7381 tgtttgggtc tactggacat ggtgtatcgt cttgcttggc gcggaaatta ctgtcactct
     7441 cggggaatac cgcaaactaa aacaagcagc tgaacaagaa gaagacgacg aaccatgatt
     7501 gcattaattc aacgcgtaac ccgtgccagc gtcaccgtgg agggagaagt gacgggcgaa
     7561 attggcgcgg gacttttggt gttattgggt gtcgaaaagg atgacgacga acagaaagca
     7621 aaccgtctgt gcgagcgtgt gctcggctat cgcatcttta gcgatgccga aggcaagatg
     7681 aacctcaacg tgcaacaggc gggcggcagt gtgctggtgg tttcccagtt taccctcgcc
     7741 gcagataccg aacgggggat gcgtccaagt ttctccaaag gtgcatcacc ggatcgcgca
     7801 gaggcgttat atgactattt cgtcgaacgc tgccgtcagc aagagatgaa cacgcaaaca
     7861 ggacgcttcg ctgcggatat gcaggtatcg ctggtcaatg atggccccgt gacattctgg
     7921 ttgcaggtat gagccagctt ccagggttgt cacgggaaac aagagagagt atcgctatgt
     7981 atcaccttcg ggttccacaa acagaagaag aattagagcg ttactatcag tttcgctggg
     8041 aaatgttgcg taagcccctg catcaaccaa aaggttcgga acgcgacgcg tgggatgcga
     8101 tggcgcatca ccagatggtc gtcgacgagc agggtaatct ggtggcggta ggccgactgt
     8161 atattaatgc cgacaatgaa gcgtccattc gctttatggc cgttcatccc gacgtgcagg
     8221 acaaagggtt aggcacgctg atggcgatga ccctggagtc ggtggcgcgt caggaaggcg
     8281 ttaagcgcgt gacctgtagc gcccgtgaag acgcggtgga gtttttcgcc aagctggggt
     8341 ttgttaatca gggagaaatc accacgccaa ccaccacgcc gattcgccat tttttgatga
     8401 ttaagcccgt cgccactctg gatgacattc tgcatcgcgg cgactggtgc gcgcagctgc
     8461 aacaggcgtg gtacgaacat atcccgctta gtgaaaaaat gggcgtgcgc attcagcaat
     8521 ataccgggca aaaatttatc actaccatgc cagaaaccgg caatcagaat ccgcaccata
     8581 cgctgtttgc cgggagttta ttctcactgg cgacgctcac cggttgggga cttatctggc
     8641 tgatgctgcg tgaacgccac ctcggcggaa cgattattct tgcggatgcg catatccgct
     8701 acagcaagcc gattagcggt aaacctcatg cggtagccga cctcggtgcc ttaagcggcg
     8761 atctcgaccg tctggcgcgc ggacgaaaag cacgggtgca gatgcaggtc gaaatctttg
     8821 gctacgagac gccgggtgca gtgtttgaag gcacgtatat cgttctgccc gcgaagccat
     8881 ttggcccgta tgaagagggc gggaacgaag aagagtagct gatggagagg ggcggtgctg
     8941 cctctctcat tcaggtcatt tatattagtc cttggctatt tcggttatat tgaataagaa
     9001 ctctataaat ttttattctt cgttggctcc atacgtgtct tgctacatca ctgttcagga
     9061 gcatttaaat attatttgta gagctgtatg gcaattatcg cagataatgc ttaattcatc
     9121 atcttagttc atgtttgagc gatttttgat agtcaaaacc ccctctggtg ccata
//
LOCUS       DAZUCE010000199         8963 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZUCE010000199 DAZUCE010000000
VERSION     DAZUCE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.199"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            39..1181
                     /gene="yghO"
                     /locus_tag="ACHY03_004006"
     CDS             39..1181
                     /gene="yghO"
                     /locus_tag="ACHY03_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFS3907184.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKIAGQIVGRITAQIDTLHRERYGKDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1257..2027)
                     /gene="glcC"
                     /locus_tag="ACHY03_004007"
     CDS             complement(1257..2027)
                     /gene="glcC"
                     /locus_tag="ACHY03_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFS3907185.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            2278..3777
                     /gene="glcD"
                     /locus_tag="ACHY03_004008"
     CDS             2278..3777
                     /gene="glcD"
                     /locus_tag="ACHY03_004008"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFS3907186.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTSILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3777..4829
                     /gene="glcE"
                     /locus_tag="ACHY03_004009"
     CDS             3777..4829
                     /gene="glcE"
                     /locus_tag="ACHY03_004009"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFS3907187.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4840..6063
                     /gene="glcF"
                     /locus_tag="ACHY03_004010"
     CDS             4840..6063
                     /gene="glcF"
                     /locus_tag="ACHY03_004010"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFS3907188.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6068..6472
                     /locus_tag="ACHY03_004011"
     CDS             6068..6472
                     /locus_tag="ACHY03_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFS3907189.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGVQDAQVAKAAAAVLAK"
     gene            6494..8665
                     /gene="glcB"
                     /locus_tag="ACHY03_004012"
     CDS             6494..8665
                     /gene="glcB"
                     /locus_tag="ACHY03_004012"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFS3907190.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTLAQFVG
                     YRGNADAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
BASE COUNT         2139 a         2279 c         2550 g         1995 t
ORIGIN      
        1 ttgtttgtca ccgttaatta tttgttaccc acagaactat ggaatgcgat ctgctgatga
       61 ttaaaattga aaaagtaatt aataaaaatg accttaaggc atttatcgct tttccgtcat
      121 cactttatcc cgacgatcca aactggatac cccctttatt cattgaacgc aacgagcatt
      181 tgtctgcgaa aaatccgggg acggaccata tcatctggca ggcgtgggtg gcaaaaatag
      241 cggggcagat agttgggcgt attaccgcgc aaatagatac cttgcaccgc gagcgttacg
      301 gcaaagatac cggtcatttc ggcatgattg acgccattga tgatccgcag gtttttgccg
      361 cgttgtttgg cgcagcggaa gcgtggttga agtcacaagg tgcaagtaag atcagcggtc
      421 ctttcagcct gaatatcaat caggaaagcg gattactgat tgaaggtttc gatacaccac
      481 cctgtgcgat gatgccacac ggcaaaccgt ggtatgccgc gcatattgaa caactgggtt
      541 atcacaaagg cattgattta ctggcgtggt ggatgcagcg aaccgatctc actttctctc
      601 cggcgctaaa aaaactgatg gatcaggtgc gcaaaaaggt gaccattcgc tgcatcaatc
      661 gtcagcggtt tgccgaagag atgcagatcc tgcgtgagat tttcaactct ggctggcagc
      721 acaactgggg atttgtgccg tttaccgaac atgaattcgc gaccatgggc gaccaactta
      781 aatatctggt gccggacgat atgatctaca tcgctgagat tgattctgca ccctgcgcgt
      841 ttattgtcgg cttgccgaac atcaacgagg cgattgccga tctgaacgga tcgctctttc
      901 ccttcggctg ggcaaaattg ctgtggcgtt tgaaagtcag cggtgtgcga actgcgcgag
      961 tgccgctgat gggcgtgcgg gacgagtatc agttcagccg catcggcccg gtgattgcgc
     1021 tgttattgat tgaagcctta cgcgatccgt ttgcccgccg gaagatcgat gcgctggaga
     1081 tgtcatggat cctcgaaacc aatacaggca tgaataacat gctggaacgc attggggcag
     1141 agccgtacaa gcgttaccga ttgtatgaaa agcagattta ataccagatc ctgtttagcg
     1201 caggggcgat ccctcgcccc tcgaggaaag ggttagggtg agggtgaaaa ctcggctcac
     1261 attcccacct tgttcatctc cagcggcacc gccgagcgaa tcaaatggtg gccttccagc
     1321 tcgatatcgt ggagattctt tttcacggtc cgcacatgat cgcgtgctgc gcgctgggcg
     1381 acgtgcggca gccgctgcaa caccgcgttg tagatccgcg catgctggcg atcgatctgc
     1441 tttttttgct gtggtcgatg gtagagatta tttacgctgg caaacactga attaaacatc
     1501 agatcggtca atgattgcag cgtaaacacc agcacctgat tgtgagaagc ctgacaaatg
     1561 gcgagatgga aagcgtgatc caactgcgca tgttcaatca gcgaaatctc tttgttgttc
     1621 tcactggcgg cgagcatttt ttcataacag cgggttatca caacaaaatc agcctgcgtt
     1681 cccagcgttg ccgccagcct tgccgattcg ccctccagta atgcgcgaac atcgagcaga
     1741 tcgtacagcg ttcgcggttg cgtactgaac agatggatca gcggactggt gtcctgcacc
     1801 cgattaagcc gtgcgacacg agaatcgcga ccctgcgccg tttcaataat cccgcgcccg
     1861 cgcagcacgg tcagcccttc acgcagtgcg gagcgtgaga agccgagctt ttcacacagt
     1921 cgacgttccg agggaagcgg ctgaccgacc ttcagtacgc cgtcgataat taaccgttcg
     1981 atactctctg caaccacttc gcaaataggg cgacgttcat ctttcattcc cggacctcgt
     2041 gcacaggtag gaccaatttt tctgttctta actcgcaaaa cacgcacatc acgtaagtga
     2101 ttatgttaca tcaatttaac attaagttaa ccaagacaag gtcacagagc tggaaaaaaa
     2161 atggtctgac cggtagctaa agagatagac gaaaacgaaa agcccgctta ataactgttc
     2221 acagaagcag cgcgcaaaaa tcagctgcca cacaacataa caaagcgaag cctactcatg
     2281 agcatcttgt acgaagagcg tcttgatggc gctttacccg atgtcgaccg cacatcggta
     2341 ctgatggcac tgcgtgagca tgtccctgga ctggagatcc tgcataccga tgaggagatc
     2401 attccttacg agtgtgacgg gttgagcgcg tatcgcacgc gtccattact ggttgttctg
     2461 cctaagcaga tggaacaggt gacatcgatt ctggctgtct gccaccgcct gcgtgtaccg
     2521 gtggtgaccc gtggtgcagg caccgggctt tctggtggcg cgctgccgct ggaaaaaggt
     2581 gtgttgttgg tgatggcgcg ctttaaagag atcctcgaca ttaaccccgt tggtcgccgc
     2641 gcgcgcgtgc agccaggcgt gcgtaacctg gcgatctccc aggccgttgc accgcataat
     2701 ctctactacg caccggaccc ttcctcacaa atcgcctgtt ccattggcgg caacgtggca
     2761 gaaaatgccg gcggcgtcca ctgcctgaaa tatggtctga ccgtacataa cctgctgaaa
     2821 attgaagtgc aaacgctgga tggcgaggca ctgacgctgg gatcggatgc gctggattcg
     2881 cctggttttg acctgctggc gttgttcacc ggatcggaag gtatgctcgg cgtgaccacc
     2941 gaagtgacgg taaaactgct gccgaagccg cccgtggcgc gggttctgtt agcaagcttt
     3001 gactcggtag aaaaagccgg acttgcggtt ggtgacatca tcgccaatgg cattatcccc
     3061 ggcgggctgg agatgatgga taacctgtcg atccgcgcgg cggaagattt tattcatgcc
     3121 ggttatcccg tcgacgccga agcgattttg ttatgcgagc tggacggcgt ggagtctgac
     3181 gtacaggagg actgcgagcg ggttaacgac atcttgttga aagcgggcgc gactgacgtc
     3241 cgtctggcac aggacgaagc agagcgcgta cgtttctggg ccggtcgcaa aaatgcgttc
     3301 ccggcggtag gacgtatctc cccggattac tactgcatgg atggcaccat cccgcgtcgc
     3361 gccctgcctg gcgtactgga aggcattgcc cgtttatcgc agcaatatga tttacgtgtt
     3421 gccaacgtct ttcatgccgg agatggcaac atgcacccgt taatcctttt cgatgccaac
     3481 gaacccggtg aatttgcccg cgcggaagag ttgggcggga agatcctcga actctgcgtt
     3541 gaagttggcg gcagcatcag tggggaacat ggcatcgggc gagaaaaaat caatcaaatg
     3601 tgcgcccagt tcaatagcga tgaaatcacg accttccatg cggtcaaggc ggcgtttgac
     3661 cccgatggtt tgctgaaccc tgggaaaaac attcccacgc tacaccgctg tgctgaattt
     3721 ggtgccatgc atgtgcatca cggtcattta ccttttcctg aactggagcg cttctgatgc
     3781 tacgcgagtg tgattacagt caggcgctgc tggagcaggt gaatcaggca attagcgata
     3841 aaacgccgct ggtgattcag ggcagcaata gcaaagcctt tttaggtcgc ccagtcaccg
     3901 ggcaaacgct ggatgtgcgt tgtcatcgcg gcattgttaa ttacgacccg accgagctgg
     3961 tgataaccgc tcgtgccgga acgccgctgg tggcgattga agcggcgctg gagagcgcag
     4021 ggcaaatgct tccctgcgag ccgccgcatt atggtgaaga agccacctgg ggcgggatgg
     4081 tcgcctgcgg gctggcgggg ccgcgtcgcc cgtggagcgg ttcggtccgc gattttgtcc
     4141 tcggcacgcg catcattacc ggcactggaa aacatctgcg ttttggtggc gaagtgatga
     4201 aaaacgttgc cggatacgat ctctcacggt taatggccgg aagctacggt tgtcttggcg
     4261 tgctcactga aatctcaatg aaagtgttac cgcgaccgcg cgcctccctg agcctgcgtc
     4321 gggaaatcag cctgcaagaa gccatgagtg aaatcgccga gtggcaactc cagccattac
     4381 ccattagtgg cttatgttac ttcgacaatg cgttgtggat ccgccttgag ggcggcgaag
     4441 gatcggtaaa agcagcgcgt gaactgctgg gtggcgaaga ggttgcgggt cagttctggc
     4501 agcaattgcg tgaacaacaa ctgccgttct tctcgttacc aggtacctta tggcgcattt
     4561 cattacccag cgatgcgccg atgatggatt tacccggcga gcaactgatc gactggggcg
     4621 gggcgttacg ctggctgaaa tcgacagccg aggacaatca aatccatcgc atcgcccgca
     4681 acgctggcgg tcatgcgacc cgctttagtg ccggagatgg tggctttgcc ccgctatcgg
     4741 ctcctttatt ccgctatcac cagcagctta aacagcagct cgacccttgc ggcgtgttta
     4801 accccggtcg catgtacgcg gaactttgag gagcaggcta tgcaaaccca attaactgaa
     4861 gagatgcggc agaacgcgcg cgcgctggaa gccgacagca tcctgcgcgc ctgtgttcac
     4921 tgcggatttt gtaccgcaac ctgcccaacc tatcagcttc tgggcgatga actggacggg
     4981 ccgcgcgggc gcatctatct gattaaacag gtgctggaag gcaacgaagt cacgcttaaa
     5041 acacaggagc atctcgatcg ctgcctcact tgccgcaatt gtgaaaccac ctgtccttct
     5101 ggtgtgcgct atcacaattt gctggatatc gggcgtgata ttgtcgagca gaaagtgaaa
     5161 cgcccactgc cggagcgaat actgcgcgaa ggattgcgcc aggtagtgcc gcgtccggcg
     5221 gtcttccgtg cgctgacgca ggtagggctg gtgctgcgac cgtttttacc ggaacaggtc
     5281 agagcaaaac tgcctgctga aacggtgaaa gctaaaccgc gtccgccgct gcgccataag
     5341 cgtcgggttt taatgctgga aggctgcgcc cagcctacgc tttcgcccaa caccaacgcg
     5401 gcaactgcgc gagtgctgga tcgtctgggg atcagcgtca tgccagctaa cgaagcaggc
     5461 tgttgtggcg cggtggacta tcatcttaat gcgcaggaga aagggctggc gcgggcgcgc
     5521 aataatattg atgcctggtg gcccgcgatt gaagcaggtg ccgaggcaat tttgcaaacc
     5581 gccagcggct gcggtgcgtt tgtcaaagag tacgggcaga tgctgaaaaa cgatgcgtta
     5641 tatgccgata aagcgcgtca ggtcagtgaa ctggcggtcg atttagtcga gcttctgcgc
     5701 gaggaaccgc tggaaaaact ggcaattcgc ggcgataaaa aactggcctt ccactgcccg
     5761 tgtaccctac aacatgcgca aaagctgaac ggcgaagtgg aaaaagtgtt gcttcgtctt
     5821 ggatttacct taacggacgt tcccgacagc catctgtgct gcggttcagc gggaacatat
     5881 gcgttaacgc atcccgatct ggcacgccag ctgcgggata acaaaatgaa tgcgctggaa
     5941 agcggcaaac cggaaatgat cgtcaccgcc aacattggtt gccagacgca tctggcgagc
     6001 gccggtcgta cctctgtgcg tcactggatt gaaattgtag aacaagccct tgaaaaggaa
     6061 taacaaaatg aaaactaaag tcattcttag ccagcaaatg gcgagtgcaa ttattgccgc
     6121 aggtcaggaa gaggcgcaga aaaataactg gtctgtttcc attgctgttg ccgatgacgg
     6181 cggtcatctg cttgcgttaa gtcgcatgga cgattgcgcg ccgattgcgg cttacatctc
     6241 ccaggagaaa gcgcgtaccg ccgcgctggg gcgtcgtgaa actaagggct atgaagagat
     6301 ggtgaacaac ggacgtaccg cgttcgtgac tgcgccgtta ttaacgtcgc tggaaggcgg
     6361 cgtaccggtt gttgttgatg ggcaaattat tggtgccgtg ggcgtttctg gtttaaccgg
     6421 agtacaggat gcgcaggtcg cgaaagcggc agcagcggtg ttggcgaaat aagcgaaaac
     6481 gaggagagaa acaatgagtc aaaccataac ccagggccgt ttacgcattg acgccaattt
     6541 taaacgtttt gtggatgaag aagttttacc gggaacaggg ctggacgctg cggcgttctg
     6601 gcgcaatttt gatgagatcg ttcacgatct ggcaccagaa aatcgtcagt tgctggcaga
     6661 acgcgatcgc attcaggcgg cgcttgatga gtggcatcgc agcaatccgg ggccggtaaa
     6721 agataaagcg gcctataaat ctttcctgcg tgaactgggc tacctggtgc cgcaaccgga
     6781 gcgcgtgacg gtggaaacca cgggcattga cagcgaaatc accagtcagg cggggccaca
     6841 gctggtagtt ccggcaatga acgcccgcta cgcgctgaac gcggcaaacg ctcgctgggg
     6901 ctcactgtac gatgcgttat acggcagcga catcatcccg caggaagggg cgatggtcag
     6961 cggctacgat ccgcaacgcg gtgagcaggt tatcgcctgg gttcggcgtt tcctcgatga
     7021 atctctaccg ctggaaaacg gcagctatca ggatgtggtg gcgtttaagg tggtcgataa
     7081 acaattacgc atccagttga aaaatggtaa agaaaccacg ttacgtaccc tggcgcagtt
     7141 tgtcggttac cgtggcaatg ccgatgcgcc gacctgcatt ttgctgaaaa ataacggcct
     7201 gcatattgag ctacaaatcg atgccaacgg gcgtattggc aaagacgatc cggcgcacat
     7261 caacgatgtt atcgtcgaag ctgctatcag taccattctc gactgcgaag attcggtcgc
     7321 ggcggttgat gcggaagata aaatcctgct gtaccgcaat ctgctgggcc tgatgcaggg
     7381 gactctgcaa gagaaaatgg agaaaaacgg tcgacaaatc gtgcgtaaac tgaatgacga
     7441 tcgtcattac accgccgccg atggctctga aatttctctg cacggacgct cgctgctgtt
     7501 tatccgcaac gtgggtcatt tgatgaccat tcctgtgatt tgggacagcg aaggcaatga
     7561 aatcccggaa ggcattcttg atggcgtcat gactggcgcg attgccctct atgatttaaa
     7621 agtgcagaaa aactcgcgca ctggcagcgt ctatattgtg aaaccgaaaa tgcacggtcc
     7681 gcaggaagtg gcgttcgcca acaaactgtt tacccgcatt gagacaatgc tcggtatggc
     7741 accgaatacc ctgaaaatgg gcattatgga tgaagaacgt cggacctcgc tgaacttgcg
     7801 tagctgtatc gctcaggcgc gcaaccgcgt ggcgttcatc aataccggtt tcctcgaccg
     7861 taccggcgat gaaatgcatt cggtgatgga agctggcccg atgctgcgta aaaatcagat
     7921 gaaatcgacg ccttggatca aagcctacga gcgtaataac gtgctttccg gtctgttctg
     7981 tgggctgcgc ggtaaagcgc aaattggtaa aggcatgtgg gcaatgccgg acctgatggc
     8041 agacatgtac agccagaagg gcgaccaact gcgtgccggg gcaaacacag cctgggttcc
     8101 gtcaccaacc gctgctacgc tccatgcgct gcactaccac caaaccaacg tacagagcgt
     8161 gcaagccaac attgcccaga ccgagttcaa tgctgaattt gaaccgctgc tggacgatct
     8221 gctgactatt ccggttgctg aaaacgctaa ctggtcggcg caagagatcc aacaagagct
     8281 ggataacaac gtgcagggga ttctggggta cgtggtgcgc tgggtggagc aggggattgg
     8341 ttgttcaaaa gtgccggata ttcacaatgt ggcgctgatg gaagaccgcg caacgctgcg
     8401 tatctccagc cagcatatcg ccaactggtt acgtcacggt attctgacca aagaacaggt
     8461 gcaggcgtcg ctggagaata tggcgaaagt ggttgatcag caaaacgctg gcgatccggc
     8521 ttatcgtccg atggcgggga atttcgctaa ctcgtgtgct tttaaagctg ccagcgattt
     8581 gatcttcctc ggcgtgaaac agccaaacgg ctataccgaa ccgttattac atgcctggcg
     8641 tttgcgcgaa aaagaaagtc attaatagcg tcaaaaaaag ccccggcaag ttttattgtc
     8701 ggggctatga tagtaactgc ggtagtacat ttatcccgga aataaccgga gcgattatca
     8761 gggtttaaca atattaaata cccgtagcgc attactagcg ggcctaaaac aacaatatgg
     8821 acatcttaac gatactctgc gcctgcgtgt gcggaacaga aacccttacc cgatatactt
     8881 gttatgccag aggtagcaat tgcgttagcg gcgtactgtt gctgtaaagt ataacgccta
     8941 tattttacag ttcagtcctc ttt
//
LOCUS       DAZUCE010000200         8922 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZUCE010000200 DAZUCE010000000
VERSION     DAZUCE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.200"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..345
                     /gene="trbF"
                     /locus_tag="ACHY03_004013"
     CDS             <1..345
                     /gene="trbF"
                     /locus_tag="ACHY03_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFS3907191.1"
                     /translation="RDIERSCDNAIKYFWLFVVFFAAGLIVRNVMHDFFSAGIDSWKA
                     DPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEIIFGGVRIFLLKRRMRRE
                     NTLREGSNNASH"
     gene            332..1705
                     /gene="traH"
                     /locus_tag="ACHY03_004014"
     CDS             332..1705
                     /gene="traH"
                     /locus_tag="ACHY03_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFS3907192.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFATNTTQ
                     AGVWQGQAAGYAYGGSLYARNQVRNIQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMANDINSMNLSSCQVAQGIVGG
                     LFPQTQESQQKVCQDIAGESNIFADWAASRQGCTVGGQSSAVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDIVRAIMEGG
                     TAKIHHCNDSDKCLKVVADANVTISKDRALKSQIAKLLTSIQNKALSDSPLAENEKTF
                     IQSTTIPVFRYLVDPLMLGVSNSVLYQLTDYIGYDIMLQYIQELLQQARAMISTGNYP
                     QAVLDSVLDNLNQAQQQIAIFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGGVQ"
     gene            1702..4524
                     /gene="traG"
                     /locus_tag="ACHY03_004015"
     CDS             1702..4524
                     /gene="traG"
                     /locus_tag="ACHY03_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFS3907193.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMVADDPYMVIFSRPSPLRGVYDSNNNFVSCKDASVTLK
                     DKLNLDTKTGGKTWHYYVQQLFGGRPDPDLLFRELLSDSYSYFYGASQSASQIMRKNV
                     TINALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSVGHVIMRNLPMVQTILTG
                     ITIGIFPLLVLAAAFNKMTLPVLKGYVFALMWLQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSEVSQIQLKYSDLASTAGYLSAMIPPLSWMMVRGLGAGFSSVYSHFASSSITPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGSMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMARESMNKAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISDEQATQELASRSTRASAGMY
                     GDAHAEWGIKPKILGVGGGAGVRGGGRAGIDWSDDDAHQASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSILGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKHKQ"
     gene            4540..5037
                     /locus_tag="ACHY03_004016"
     CDS             4540..5037
                     /locus_tag="ACHY03_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HFS3907194.1"
                     /translation="MMMKNSAHIALITVIQLIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSTGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCV
                     SVREWGGNQLKNQYDGGEKLEFGFVEYTPTTYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YDDRR"
     gene            5069..5800
                     /gene="traT"
                     /locus_tag="ACHY03_004017"
     CDS             5069..5800
                     /gene="traT"
                     /locus_tag="ACHY03_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFS3907195.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQSWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSKVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            5860..6120
                     /locus_tag="ACHY03_004018"
     CDS             5860..6120
                     /locus_tag="ACHY03_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907196.1"
                     /translation="MNRAESRLSQVLFLLGYDSEKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            6288..7019
                     /locus_tag="ACHY03_004019"
     CDS             6288..7019
                     /locus_tag="ACHY03_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907197.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTATTKDSLYSNNI
                     DTDLLVVIGGLVVILFLMMLVMYFINSLRHKELTEILTKISFPEKSGSNPLKPEEGRC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAQEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDLINKTSRVSPDSGHFYEPGTALDYRERVRLFIQIQFSHESDWSEILRLTDIV
                     LEKVSSGRSAGGYHHRYTGYTWFVL"
     gene            7075..>8922
                     /gene="traD"
                     /locus_tag="ACHY03_004020"
     CDS             7075..>8922
                     /gene="traD"
                     /locus_tag="ACHY03_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFS3907198.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHSGESFTIRDWMRGVREDKQNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
BASE COUNT         2283 a         2056 c         2403 g         2180 t
ORIGIN      
        1 cgggatattg agcgaagttg tgataacgcc ataaaatact tctggctttt tgtggttttc
       61 tttgctgccg gattgattgt aaggaacgtt atgcatgatt ttttcagtgc cggtattgat
      121 tcgtggaaag ctgacccgga attaaataat tttcgttata tgtggaacac cctgatgtat
      181 gtcattccga ttatgttata tgcgcttgca gctggttttc ttgctgcggc ttctctgtcg
      241 ccgttgtgcg aaataatatt tgggggtgtc cggatattcc tgctgaaacg ccgtatgcgc
      301 cgggagaaca cattaaggga gggcagcaat aatgcttcgc attaagcctc tttttattct
      361 ttgtgccgcc ttactgacgg tcacacctgc agcgtcagcg gatgtgaaca gcgacatgaa
      421 ccagttcttt aacaagctgg gctttgccac caataccacc caggccggag tctggcaggg
      481 gcaggctgcc gggtatgcct atggcggttc tctgtatgcc cgtaatcagg tcagaaatat
      541 ccagcttatc tccatgacac tgcccgacat caatgcaggg tgtggtggca tcgatgccta
      601 tctcggctca ttcagtttta ttaatggcga gcagctgcag cgttttgtta agcagattat
      661 gagtaacgcg atggggtact tttttgacct tgccctgcag acaacggtgc ccgaaatcaa
      721 aaccgcgaaa gacttcctgc agaagatggc gaatgacatt aacagtatga acctcagtag
      781 ctgtcaggtg gcccagggga tagttggcgg gttgtttcct cagacgcagg agtcgcagca
      841 gaaggtctgc caggatattg caggtgagag caatatcttc gctgactggg ccgcttcccg
      901 tcaggggtgc acggtcggag ggcagtcttc tgcggtgcgt gacaaggcca gcgataagga
      961 caaggagcgg gtactgaaaa acatcaatat catctgggac tcgctgtcca aaaacaggat
     1021 gcttgatggt aacaaagagc tgaaagagtt cattatgacg ctcacaggta cgctgatttt
     1081 tggtgaagac agtgagatta cccctcttcc tgcacgaacc actgaccgcg atattgtccg
     1141 agcgattatg gaggggggaa cggcaaaaat tcaccactgc aacgattcgg acaagtgtct
     1201 gaaggtggtg gcggatgcaa atgtgaccat cagcaaggac agggcgctca agtcacagat
     1261 agcgaaactg ctcaccagta ttcagaacaa ggcgctcagt gattctccgc tggctgagaa
     1321 cgaaaaaacc ttcattcaga gcaccaccat tcctgttttc agatatctgg ttgacccact
     1381 gatgctgggc gtatccaaca gtgttctcta ccagctgacg gactatatcg gttacgacat
     1441 catgctccag tacattcagg aactgcttca gcaggcccgg gcgatgattt ccaccgggaa
     1501 ctacccgcag gcggtcctgg acagtgtgct cgataacctt aatcaggcgc agcaacagat
     1561 tgccattttc cagagccagg tgcaggtcca gcaggatgca ttgctggtcg ttgaccgtca
     1621 gatgagctac atgcgccagc agttgtctgc ccgtatgctc agccgttacc agaataatta
     1681 tcacttcggg ggaggcgtac agtgaacgag gtctatgtca tcgccggtgg ggaatggtta
     1741 cggaacaacc tgaatgccat cgctgccttt atgagtaccc ggacgtggga ttccatcgaa
     1801 aaaattgcgc tcacattatc tgttctcgca gtggctgtga tgtgggtaca acggcacaac
     1861 gtgatggatt tgctgggatg ggttgccgtg tttgtactta tcagtctgct ggttaatgtc
     1921 cgcacatcgg tgcagattat tgataacagt gacctggttc aggtccatcg tgtggataat
     1981 gtaccggtcg ggctggcgat gccactttca ctgacgaccc gtatcgggca tgcgatggtg
     2041 gccggctatg agatggtttt cgctcagccg gacagcgcca cctacagcaa gacaggcatg
     2101 ctgttcgggg cgaacctgat tgtgaaaagc accgatttcc tgtcccggaa tccggaaatc
     2161 atcaatctgt ttcaggacta cgtccagaac tgcgtgctgg gtgatattta cctgaaccac
     2221 aaatacacgc tggaagacct gatggtggct gatgacccct atatggtgat tttttcccgg
     2281 cccagcccgc tgcggggggt atatgacagt aataataatt ttgtcagctg caaggatgcg
     2341 tcggtcacgc tgaaagacaa gctgaatctt gatacaaaga ccggaggcaa gacctggcat
     2401 tattatgtgc agcagttatt tggtggcaga ccggaccctg acctgttatt cagggaactg
     2461 ctcagtgaca gttacagcta tttctacggg gccagccagt cggccagtca gattatgcgt
     2521 aagaatgtga caataaatgc actgaaagag ggtatcacca gtaatgcggc ccgtaacggt
     2581 gatacggcca gcctggtcag tctggccacc acctcatcaa tggagaaaca gagactggcg
     2641 cacgtctctg tcggacatgt aataatgcga aacctgccga tggtccagac cattctgacc
     2701 ggaataacca ttggtatttt cccgcttctg gtactggctg ctgcatttaa taaaatgaca
     2761 ctgccggtgc tgaagggcta tgtgtttgcc cttatgtggc tgcagacatg gcctctgctg
     2821 tacgccattc tgaacagtgc catgacattc tatgcgaagc agaacggtgc cccggtggtg
     2881 ttgtcggaag tctctcagat acagctgaaa tactccgacc tggcatcaac cgcaggttat
     2941 ctctcagcga tgatcccccc tctttcgtgg atgatggtac gaggccttgg tgccggattt
     3001 tccagcgtat acagccactt tgcgtcttct tccatcacgc caaccgccag tgcggcaggc
     3061 agtgtggttg acggtaatta ctcctacggc aacatgcaga cggaaaacgt gaacggcttc
     3121 agctggagca ccaacagcac cacgtcgttt ggtcagatga tgtaccagac tggcagtggc
     3181 gcaaccgcca cacagacccg tgacggcagt atggtgatgg acgccagtgg ggcaatgtcc
     3241 cgtttgccgg ttggtattaa tgcaacgcgt cagattgcag cagcgcaaca ggaaatggcc
     3301 cgggagtcga tgaataaggc ggaaagtgcc ctgcatggtt tcagcagcag tattgccagt
     3361 gcctggaaca cactcagcca gtttggctct aaccggggaa gcagtgattc agtcaccagt
     3421 ggcgctgaca gcacgatgag cgcgcaggac tccatgatgg ccagccgtat gcgcagcgca
     3481 gtggaaagct atgcgaaggc gcataatatc agtgatgagc aggcgactca ggagctggca
     3541 tccagaagta cgagagcttc tgccgggatg tatggagatg ctcatgctga atgggggatt
     3601 aaacctaaaa ttcttggtgt tggtggtggc gcaggtgtca gaggtggtgg tcgggcaggt
     3661 attgactgga gcgatgatga tgcgcatcag gccagcagtg gttcacgggc cagtcatgat
     3721 gcccgtcatg atatcgatgc cagggctaca caagacttta aagaggcaag cgattacttt
     3781 accagtcgca aggtcagtga atccggcagc catactgaca ataatgccga ttccagggtg
     3841 gaccaactgt ctgcagccct gaactcagcg aaacagagtt acgaccagta cacgacgaat
     3901 atgacccgca gccatgaata tgctgaaatg gcctcccgta ctgaaagcat gagtgggcag
     3961 atgagtgaag acctgtcaca acagtttgca cagtatgtga tgaaacacgc gccgcaggat
     4021 gcagaagcta ttctgacaaa caccagttca ccggagattg cagaacgtcg tcgggcaatg
     4081 gcctggtctt ttgttcagga acaggtacag cctggtgttg ataacgcctg gcgtgaatcc
     4141 cgtggcgata tcggtaaagg aatggagagc gtaccttcgg gcggtggcag ccaggatatt
     4201 attgcagacc atcaggggca tcagggtatt attgagcaaa gaacgcagga cagtaatatc
     4261 cgtaatgatg taaaacatca ggttgataat atggtcacag aataccgtgg caatatcgga
     4321 gatactcaga acagcatcct cggcgaggaa aatattgtaa gagggcagta ttctgaactg
     4381 caaaatcatc ataaaacaga ggcattatct cagaataata aatataatga agaaaagtcg
     4441 gctcaggaaa gaatgcctgg tgctgacagc ccacaggaac tgatgaaaag agcaaaggag
     4501 tatcaggata agcacaagca ataatattgt atgggggaga tgatgatgaa aaattcagct
     4561 catattgctc tgataacggt gatacagctc attgcctgtt atctggctga ttgggggagc
     4621 gctgaaacag tttttatcct gtgctttatt gtactatggc agggattgtt tatatggttg
     4681 ttttctcaaa tacgaaaaaa acggcatgtg tcggatgagt ttaagtttag tacaggcgtt
     4741 tggtatataa cgatgccagt atcttcctta ctctctccgt tattaagcct gatggttttt
     4801 atcattggta cactatacga attaaggcga gttagtggtt gtgtaagtgt aagagaatgg
     4861 ggcgggaatc aacttaaaaa ccaatatgat gggggtgaaa agttagagtt cgggttcgtt
     4921 gaatacaccc caactaccta ctataaccct tcaaccggat atcctatgca tggaggattt
     4981 gattcagcag gaaatacttt tgggactcgt tggcaggact atgatgatag gcgataagaa
     5041 aactgtagct tgtaataaag gtaatttgat gaaaacaaaa aaattgatga tggttgcact
     5101 ggtcagttcc actctggccc tttcagggtg tggtgcgatg agcacagcaa tcaagaagcg
     5161 taatcttgaa gtcaaaactc agatgagtga aaccatctgg ctggagcctt ccagtgaacg
     5221 caccgttttc ctgcaaatca aaaacacatc tgacaaggat atgagcggat tgcaggcaaa
     5281 aatcagtgat gccgttaaag ccaaaggcta ccaggttgtg acctcacctg acaaggcata
     5341 ctactggatc caggccaatg tcctgaaggc agacaaaatg gatttgcggg agtctcagag
     5401 ctggctgaac agcggttatg aaggtgccat tgccggtgca gcattgggca ccggcatcac
     5461 aggatataac tccagctctg ccggtgcggc gctgggcgta gggattgcca cagggttgat
     5521 tggtatggct gccgatgcaa tggtggagga tatcaactac accatgatca cggatgtgca
     5581 gattgcagag cggacaacat cgaaagtcag aactgataat gttgcagctc tccgtcaggg
     5641 aacctccggt gcaaaagtgc agaccagcac atcaaccgga aatcaacata aataccagac
     5701 ccgtgtggta tccaatgcca acaaggttaa cctgaaattt gaagaagcga agccagtact
     5761 ggaagaccag ctggctaaat ccatcgcgaa catcctgtaa attacccatc cggcccggac
     5821 agattgtacc gggccgatat tcctgccccg gatgaataca tgaaccgtgc agaatctcgt
     5881 ctctcgcagg tactctttct actgggctat gacagtgaaa agtatcctga gcatgtatgt
     5941 cgggagttat taaaagaact ttaccggttt gctgatgcaa ccggagattc tgaactggcc
     6001 atagcctgcc agagatacct gacagcactg gaaccgttct ctgcatatga gatcggcgta
     6061 agcgcaacag aatatcttcc tgtgagtgaa tatccgaccg gacgtcgcag aagaaaatag
     6121 ctgacgcctc gcgttgctcg ttgtccaacc ccggaaagta acaaaataaa ttttgtcact
     6181 tcccggcgtt tccgaaataa atatgagaac actgaacggt tgtgcaaatt ctttgtgcga
     6241 ccgtgaagtg ttgccggcga agccggaagc ttccggagaa agtcattatg acatcagaca
     6301 atatcgctga atatgttggt gagaataatc aatttactga gaagcctgta actgaatacc
     6361 ccccggtgat acaggataat acagcgacca ctaaagactc tctgtacagc aataatattg
     6421 acactgacct tttagtggtt atcggagggc ttgttgttat attgtttctc atgatgcttg
     6481 tcatgtactt tattaattct ctcaggcata aggaattaac tgaaatactg acaaaaatat
     6541 cttttcctga gaaatccggg agcaatccgc ttaagccgga agagggccgc tgtaatttgt
     6601 tgtccctgtg tgtgctggcc ccggtattct gtacccccaa aaagctggca gaagtctcca
     6661 gacagatcat cgtgaccagt gcgcaggaaa ttgcctggac gcccgggagt agccgtattg
     6721 atggtgagca ggaagaaaat attacctggt ctctgtcgtc acagactcat gccgataaat
     6781 acgatctcat caataaaacg tcccgtgttt ctcctgacag tggccacttt tatgaaccgg
     6841 gcacggctct ggattatcgt gaacgggtac gcctttttat tcagatccag ttttcacacg
     6901 agagtgactg gtctgaaata ttgcgcctga ctgatatcgt gctggaaaaa gtttcttccg
     6961 gcaggtcagc aggcggctat caccaccggt atacagggta tacatggttt gttctgtgaa
     7021 tgtattccac accgctcttt tctgctctgt acctgacttt accggagttt catcatgagt
     7081 tttaacgcaa aggatatgac ccagggcggt cagattgcgt ccatgcgtat ccgcatgttc
     7141 agccagatcg ccaatatcat gctttactgc ctgtttattt ttttctggat actcgttggt
     7201 ctggttttat gggtaaaaat aagctggcag acgtttgtga acggctgtat ttactggtgg
     7261 tgtaccacgc tggaaggcat gcgggattta atcaggtccc agccggtcta tgagatccag
     7321 tattacggca aaaccttccg gatgaacgcg gctcaggtac tgcatgataa atacatgatc
     7381 tggtgcggtg agcagctatg gtctgcattc gttctggcgt ctgtcgtggc cctggttatc
     7441 tgcctgatca ccttctttgt tgtctcctgg attctggggc gtcagggtaa acagcagagc
     7501 gaaaatgaag tcacaggtgg tcgtcagctg actgacaatc cgaaagacgt tgcccggatg
     7561 ctgaaaaaag acggcaagga ctccgatatc cggattggcg acctgccgat tatccgggat
     7621 tctgaaatcc agaacttttg tctgcacgga acagtatcga cgggtaagtc agaggttatc
     7681 cgacgtcttg ccaactatgc ccgtaaacgt ggtgatatgg tggtgattta tgaccgttcg
     7741 tgtgagtttg tcaaaagtta ctatgatccc tccattgata agatcctgaa tccgctggat
     7801 gcacgctgtg ccgcctggga tttatggaag gagtgtctga cacagccgga ttttgataat
     7861 gtcgcgaaca cgctgatccc gatgggcaca aaagaggacc cgttctggca gggctccggg
     7921 cgcaccatct ttgcggaagc ggcgtacctg atgcgtaatg accccaaccg cagctacagc
     7981 aaactggtgg atacactgct ttccatcaaa attgaaaaac tgcgtaccta tctgcgtaac
     8041 tcaccggcgg ctaacctagt ggaagagaaa atcgagaaaa cggcgatttc catccgtgct
     8101 gtgctgacaa actacgtgaa agccatccgt tacctgcagg ggattgagca cagcggtgag
     8161 tccttcacta ttcgtgactg gatgcgtggt gtccgggaag ataagcaaaa cggctggttg
     8221 tttatctcgt cgaatgccga cacccatgcc tccctgaagc cggtggtttc catgtggctg
     8281 tccattgcca ttcgtggtct gctggcaatg ggagaaaacc gtaaccgtcg tgtgtggttt
     8341 ttctgtgatg agttacccac gctgcataaa ctgccggatc tggtggagat cctgccggaa
     8401 gcccgtaagt tcggtggctg ttatgtgttt ggtattcagt cctatgccca gctggaagat
     8461 atctacggtg agaaagcggc ggccacgctg tttgatgtgc tgaatacccg agcctttttc
     8521 cgctctccga gtcataagat tgcagagttc gctgccggtg aaattggtga aaaagagcac
     8581 ctgaaagcca gcgagcagta ttcctacggt gcagacccgg tacgtgacgg ggtatcaacc
     8641 ggtaaggaga tggagcgcca gacgctggtc agttattccg acattcagtc tctgccggat
     8701 ctgacctgtt atgtcaccct gcccggaccg tatccggcag taaaactctc cctgaaatat
     8761 caggcacgac cgaaggttgc tccggagttt attccgcgtg acatcaatcc ggaaatggag
     8821 aaccgtctga gtgccgtact tgctgcacgg gaagcagaag ggcgccagat ggccagcctc
     8881 ttcgaaccgg aggttgcctc cggagaagac gtgactcagg ct
//
LOCUS       DAZUCE010000201         8887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZUCE010000201 DAZUCE010000000
VERSION     DAZUCE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.201"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            224..1327
                     /gene="ompC"
                     /locus_tag="ACHY03_004021"
     CDS             224..1327
                     /gene="ompC"
                     /locus_tag="ACHY03_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFS3907199.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDIGSIDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIGTRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1439..2494
                     /gene="apbE"
                     /locus_tag="ACHY03_004022"
     CDS             1439..2494
                     /gene="apbE"
                     /locus_tag="ACHY03_004022"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFS3907200.1"
                     /translation="MEISFTRVALLAATLFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2568..3632
                     /gene="ada"
                     /locus_tag="ACHY03_004023"
     CDS             2568..3632
                     /gene="ada"
                     /locus_tag="ACHY03_004023"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFS3907201.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSIVNAGFP
                     DSSSYYRKANETLGMTAKQFRHGGENLAVRYALADCALGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3632..4282
                     /gene="alkB"
                     /locus_tag="ACHY03_004024"
     CDS             3632..4282
                     /gene="alkB"
                     /locus_tag="ACHY03_004024"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFS3907202.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDYRYNLTFRQAGKKE"
     gene            4358..6001
                     /gene="yojI"
                     /locus_tag="ACHY03_004025"
     CDS             4358..6001
                     /gene="yojI"
                     /locus_tag="ACHY03_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFS3907203.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKSVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6219..7865
                     /gene="mqo"
                     /locus_tag="ACHY03_004026"
     CDS             6219..7865
                     /gene="mqo"
                     /locus_tag="ACHY03_004026"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFS3907204.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8354..8842)
                     /gene="eco"
                     /locus_tag="ACHY03_004027"
     CDS             complement(8354..8842)
                     /gene="eco"
                     /locus_tag="ACHY03_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFS3907205.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         2117 a         2243 c         2421 g         2106 t
ORIGIN      
        1 gcttatttcg ccattccgca ataatcttaa aaagttccct tgcatttaca ttttgaaaca
       61 tctatagcga taaatgaaac atcttaaaag ttttagtatc atattcgtgt tggattattc
      121 tgcatttttg gggagaatgg acttgccgac tgattaatga gggttaatca gtatgcagtg
      181 gcataaaaaa gcaaataaag gcatataaca gagggttaat aacatgaaag ttaaagtact
      241 gtccctcctg gtcccagctc tgctggtagc aggcgcagca aacgctgctg aagtttacaa
      301 caaagacggc aacaaattag atctgtacgg taaagtagac ggcctgcact atttctctga
      361 caacaaagat gtagatggcg accagaccta catgcgtctt ggcttcaaag gtgaaactca
      421 ggttactgac cagctgaccg gttacggcca gtgggaatat cagatccagg gcaacagcgc
      481 tgaaaacgaa aacaactcct ggacccgtgt ggcatttgct ggtctgaaat tccaggacat
      541 tggttctatc gactatggtc gtaactacgg cgtagtttac gacgtaactt cctggaccga
      601 cgtcctgcca gaattcggtg gcgacaccta cggttctgac aacttcatgc agcagcgtgg
      661 taacggcttt gcgacctacc gtaacactga cttcttcggt ctggttgatg gcctgaactt
      721 tgctgttcag taccagggca aaaacggtag cgtaagcggc gaaggcatga ccaacaacgg
      781 tcgtgatgct ctgcgtcaga acggcgacgg cgttggcggt tctatcactt atgattacga
      841 aggcttcggt atcggtgctg cagtttccag ctccaaacgt actgatgctc agaacaccgc
      901 tgcttacatc ggtaacggcg accgtgctga aacctacact ggtggtctga aatacgatgc
      961 taacaacatc tacttggctg ctcagtacac ccagacctac aacgcaactc gcgtaggttc
     1021 cctgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc agttcgactt
     1081 cggtctgcgc ccgtctgtag catacctgca gtctaaaggt aaaaacttgg gcaccatcgg
     1141 tactcgtaac tacgacgacg aagatatcct gaaatatgtt gatgttggtg caacctacta
     1201 cttcaacaaa aacatgtcca cctacgttga ctacaaaatc aacctgctgg acgacaacca
     1261 gttcactcgt gacgctggca tcaacactga taacatcgta gctctgggtc tggtttacca
     1321 gttctaatct cgattgatat cgaacaaagg gcctgcgggc ccttttttca ttgttttcag
     1381 cgtacaaaca cagttttttg gtgtactctt gcgaccgttc gcatgaggat aatcacgtat
     1441 ggaaataagc tttacccgcg tggcactgct ggctgccacg ctcttctttg ttggttgcga
     1501 tcaaaaacca caacccgcca aaacccacgc tactgaagtt accgttcttg aaggcaaaac
     1561 catgggtacc ttctggcgtg ccagcatccc gggcattgat gccaaacgca gtgccgaact
     1621 taaagaaaag attcagaccc agctggacgc cgacgatcag ctgctttcga cctataaaaa
     1681 agattccgcg ctgatgcgct ttaacgactc gcaaagtttg tcgccgtggc cggtaagtga
     1741 agcgatggcc gatatcgtca ccacctcgct gcgcattggc gcaaggaccg atggtgcgat
     1801 ggatataacc gtcgggccgc tggtgaatct gtggggcttt ggcccggaac aacagccggt
     1861 tcaaattccg agccaggaac agatcgatgc gatgaaagcc aaaaccggct tacagcacct
     1921 gacggtcatt aatcagtcgc atcagcaata tctgcaaaaa gacctaccgg atttatatgt
     1981 cgatctctcc accgtcggtg aaggttatgc agcggatcac ctggcacgct tgatggagca
     2041 ggaagggatt tcccgctatc tggtgtcggt gggcggcgcg ctgaacagcc gtggtatgaa
     2101 cggtgaaggc cagccgtggc gggtagcgat tcaaaaacca accgataaag aaaacgcggt
     2161 tcaggctgtg gtggatatca acggtcatgg gattagcacc tctggcagct accgtaacta
     2221 ttacgaactg gacggcaaac gtctttccca tgttatcgat ccgcaaaccg ggcgtcccat
     2281 cgaacacaat ctggtatccg tgacggtgat tgctccgacg gcgctggaag ccgatgcctg
     2341 ggatactggc ttgatggtac tcgggccgga gaaagccaaa gaggttgttc gccgggaagg
     2401 gctggcggtt tatatgatca ccaaagaagg cgatagcttt aaaacctgga tgtcaccaca
     2461 gtttaaaagc ttccttgtca gcgaaaaaaa ttaaagcgca agattgttgg tttttgcgtg
     2521 atggtgaccg ggcagactaa aggctatcct taaccaggga gctgattatg aaaaacgcca
     2581 catgcttaac tgacgatcaa cgctggcaat ctgtcttagc ccgcgacccg aatgccgacg
     2641 gcgaattcgt tttcgccgtg cgcaccacag gcatcttttg ccgtccgtct tgccgcgcca
     2701 gacatgcctt gcgggaaaat gtctccttct acgcaaatgc cagcgaggca ctcgccgccg
     2761 gatttcgccc ctgcaaacgc tgccaacctg ataaagccaa tgcccagcaa catcggttgg
     2821 ataaaatcac ccacgcgtgt cgactgctgg aacaggaaac gcctgtaacg ttggaagcct
     2881 tagccgacca ggtggcgatg agtccatttc atctacatag gttgtttaaa gcgactaccg
     2941 gaatgacgcc taaagcctgg caacaggcct ggcgcgctcg ccgtttgcgc gaatcgctgg
     3001 cgaaagggga gagcgtgacg acgtctattg ttaacgccgg attccccgac agcagcagtt
     3061 actaccgcaa agccaatgaa acgctgggca tgacggctaa acaattccgt cacggtggcg
     3121 aaaatctggc ggtgcgttac gcgctggctg attgtgcgct ggggcgttgc ctggtggcag
     3181 aaagcgagcg ggggatttgc gcgatattgc tgggcgatga tgacgcgaca ctaatcagcg
     3241 agttgcagca gatgtttccc gctgccgaca acgcgcctgc cgatctgacg tttcagcaac
     3301 atgtgcgtga agtgatcgcc agcctcaatc aacgcgatac gccgctgacg ttaccgctgg
     3361 atattcgcgg cactgctttt cagcaacaag tctggcaggc actgcgcacg ataccttgcg
     3421 gtgaaaccgt cagttatcag caactggcta acgccatcgg caaaccgaaa gcggtacggg
     3481 ccgttgccag cgcctgtgcc gccaacaagc tggctatcgt aataccctgt catcgagtgg
     3541 tccgtggtga tggcacactt tccggttacc gctggggcgt gtcgcgtaaa gcgcaactgc
     3601 tgcgccgcga agctgaaaat gaggagaggt aatgttggat ctgtttgccg atgctgaacc
     3661 gtggcaagag ccactggcgg ctggtgcggt aattttacgg cgttttgctt ttaacgctgc
     3721 ggagcaacta atccgcgata ttaatgacgt tgccagccag tcgccgtttc gccagatggt
     3781 cacccccggg ggatatacca tgtcggtggc gatgaccaac tgtgggcatc tgggctggac
     3841 gacccatcgg caaggttatc tctattcgcc cattgatccg caaacaaata aaccgtggcc
     3901 cgccatgccg cagagttttc ataatttatg tcaacgtgcg gctacggcgg cgggctatcc
     3961 agatttccag ccagatgctt gtcttattaa ccgctacgct cctggcgcga aactgtcgct
     4021 gcatcaggat aaagacgaac cggatctgcg cgcgccgatt gtttctgttt ccctgggctt
     4081 acctgcgatt tttcaatttg gtggtctgaa acgaaatgat ccgctcaaac gattgttgtt
     4141 ggaacatggc gatgtggtgg tatggggcgg tgagtcgcgg ctgttttatc acggcattca
     4201 accgctgaaa gcggggtttc atccactcac cggcgactac cgctacaacc tgacattccg
     4261 tcaggcaggt aaaaaagaat aaaaataaga attattattg ctgtgcgcgc gaagatgttt
     4321 aaactgcggg ctgtaattca ttgtccgggt tttctgcatg gaacttcttg tacttgtctg
     4381 gcggcagtat cgctggccat ttatcagtgt gatggcgcta agcctcgcca gtgcggcttt
     4441 aggcattggc ttaattgctt ttatcaatca gcgccttatc gaaacggcgg ataccagtct
     4501 gctggtgttg ccggagtttc tgggattatt gctgctgttg atggcagtca ctctcggatc
     4561 gcaactggcg ctcaccactt tggggcatca cttcgtttac cgactgcgta gtgaatttat
     4621 caagcggatt ctggataccc acgtcgagcg cattgaacaa ctcggtagcg cctcgttgct
     4681 ggcgggatta accagcgatg tgcgcaatat caccattgct tttgtgcgtc tgccggaact
     4741 ggtgcagggg atcattctca ctatcggttc cgcggcgtat ctgtggatgc tgtcgggcaa
     4801 aatgttgctg gtaacggcta tctggatggc agtcaccatc tggggcggtt ttgtgctggt
     4861 ggcgcgggtg tacaaacata tggcgaccct gcgtgaaacc gaagacaagc tgtacacgga
     4921 ttttcaaacc gtactcgaag ggcgcaaaga gctgactctg aaccgggaac gcgccgagta
     4981 tgtgtttaac aacctctaca ttcctgatgc gcaagagtat cgccatcata ttatccgcgc
     5041 agacaccttc catcttagtg ccgtgaactg gtcaaacatc atgatgctgg gcgctatcgg
     5101 cctggtgttc tggatggcga acagtctcgg ttgggctgat accaacgttg ccgcgaccta
     5161 ttcgttgacg cttttattcc tgcgtacgcc gctgctttcg gcggttggcg cattgccgac
     5221 gctgctgacg gcgcaggtgg cgtttaacaa gctgaacaaa ttcgcgctcg cgcctttcaa
     5281 agcagagttt ccgcgcccgc aggcgtttcc caactggcaa acgctggagc tgcgtaacgt
     5341 gacgtttgct tatcaggata acgcgttttc cgttggtccg attaatctca ccatcaaacg
     5401 tggcgagctg ctgtttctga ttggcggcaa cggtagcgga aaatcgacgc tggcgatgtt
     5461 gttgacgggc ttgtatcagc cacaaagcgg cgaaatcttg ctggatggca aatctgtcag
     5521 cggcgaacaa ccggaagatt atcgcaaact gttttcggca gtgtttaccg atgtctggct
     5581 gtttgatcaa ctgctggggc cggagggtaa acccgctaac ccgcaactgg ttgagaagtg
     5641 gctggcgcag ctgaaaatgg ctcataagct tgagttaagc aacgggcgta ttgttaacct
     5701 gaagttatca aaagggcaga aaaaacgcgt ggcgctgttg ctggcgctgg cagaagaacg
     5761 cgatattatc ctgctggatg aatgggcggc ggatcaggat ccacacttcc gtcgtgagtt
     5821 ttatcaggtg ttgctgccgc tgatgcagga gatgggtaaa actattttcg ctatcagtca
     5881 tgatgatcat tactttatcc acgctgaccg cctgctggaa atgcgcaacg ggcaacttag
     5941 cgagctgacg ggcgaagagc gcgatgccgc ttcgcgtgat gccgttgccc ggacggcata
     6001 atattacgac gctgacctgg cggactgctg ccgccaggtc aatatgctaa aaatcctttc
     6061 atttctaata accctataat taatcaacga aattataatg tttctaaaat tagaatataa
     6121 tttataaaca ttatttaaat gttgttactt aagtgttacc gttgatgccg cgcaaatctc
     6181 acctgcaata aagcgactaa aagtaaggca ttaacaagat gaaaaaagtg actgccatgc
     6241 tcttctcgat ggccgtgggg cttaatgccg tttcgatggc ggcaaaagcg aaagcgtccg
     6301 aggagcagga aactgatgta ctgttgattg gcggcggcat tatgagcgcc acgttgggga
     6361 cctatttacg cgagctggag cctgaatggt cgatgaccat ggtggagcgc ctggagggtg
     6421 tcgcgcagga gagttcgaac ggctggaata acgccggaac cgggcattct gcactgatgg
     6481 aactgaacta caccccgcaa aacgccgatg gcagcatcag tattgaaaaa gcagtcgcca
     6541 ttaacgaagc atttcagatt tcccgccagt tctgggcgca ccaggtcgag cgcggcgtgc
     6601 tgcgtactcc gcgttcattt atcaataccg ttccgcatat gagctttgtc tggggcgagg
     6661 ataacgtcaa tttcctgcgc gcccgttacg ccgcgttgca acaaagctcg ctgtttcgcg
     6721 gtatgcgtta ctctgaagat cacgcgcaga tcaaagagtg ggcaccgtta gtgatggaag
     6781 ggcgcgatcc gcaacagaaa gtggcagcca cgcgtacgga aattggtacc gatgtgaact
     6841 acggcgagat cacccgccag ttaattgctt ccttgcagaa gaaatctaac ttctcgctgc
     6901 aactcagcag cgaagtccgc gccctaaagc gtaatgacga taacacctgg accgtcaccg
     6961 ttgccgatct gaaaaatggc actgcacaga acatccgtgc gaaatttgtc tttatcggcg
     7021 cgggcggtgc ggcactgaag ctgttacagg aatcggggat tccggaagcg aaagactacg
     7081 ccggtttccc ggtgggcgga cagttccttg tttcggaaaa cccggacgtg gttaatcacc
     7141 atctggcgaa ggtttacggt aaagcatccg ttggcgcacc accgatgtcg gttccgcata
     7201 tcgatacccg cgttctggac ggtaaacgcg tagtgctgtt tgggccattt gccaccttct
     7261 caaccaaatt cctcaaaaac ggttcattgt gggatctgat gagttccacc accacctcta
     7321 acgtgatgcc gatgatgcac gtcgggctgg ataatttcga tctggtgaaa tatctggtga
     7381 gtcaggtgat gttgagtgaa gaggatcgtt ttgaagcgtt gaaagagtac tatccgcaag
     7441 cgaaaaaaga ggactggcgt ttgtggcaag cggggcagcg tgtgcagatt atcaagcgtg
     7501 atgccgataa aggtggtgta ctgcgtctgg gtactgaagt ggtcagtgac cagcaaggaa
     7561 ccattgccgc gctcctgggg gcatcgccag gggcgtcaac cgccgcgccg attatgttgg
     7621 atctgctgga aaaagtattt ggcgatcgcg tttccagccc gcaatggcag gctacgttga
     7681 aagcgatcgt tccgtcttat ggacgcaagc tgaacggtga tgtagcggca acagaacgcg
     7741 agttgcagta caccagcgaa gtgctgggcc tgaaatacga caagccgcag gcagcagata
     7801 gtacgccgaa accgcagttg aaaccgcaac ccgttcaaaa agaagtggcg gatattgcgt
     7861 tgtaatgatg tgccacatcc ggcatggtat gccggatgtg gcgtatgctg ataagacgcg
     7921 ccagcgtcgc atcaggcaac cggctcgggc gttagatgtt ggatgcggcg taaccgcctt
     7981 atccaaccta tggggtggag cacttcacag cggtgtaggc ctgataagac gcgtcagcgt
     8041 cgcatcaggc aaccggctcg gacgttggat gtcggatgcg gcgtaaccgc cttatccaac
     8101 ctacggggtg ccgcacttca cagcggtgta ggcctgataa gacgcgtcag cgtcgcatca
     8161 ggcaaccggc tcggacgttg gatgtcggat gcggcgtaac cgccttatcc gacctacggg
     8221 gtggagcact tcacagcggt gtaggcctga taagacgcgc cagcgtcgca tcaggcaacc
     8281 ggctcgggcg ttagatgttg gatgcggcgt aaccgcctta tccaacctat ggggtgccgc
     8341 acttcacagc agtttagcga actaccgcgt tgtcaatttt ctcttccgcc ttccagacgc
     8401 ggtacttcac atctacattg tctggcgtat acaccacgat cggcagcttg ctgttgtaac
     8461 gcagcattcc agcatcgccc agatacgcgg tgacaaattt cttctctttc ttgccatccg
     8521 ggcaggccat catcgtggaa accggcgagc taactttatc aaagacataa taatcatagc
     8581 cccagccttc cagcgttttg ctttccagct tcccgccgag acgatgcaaa ttgcaatcga
     8641 cttccagcgt ctgaccgatt aacagttcta ctttcagggt agattcatct tcttgcgggg
     8701 ttaactgaat cacctgacgc ttcatgcctt tttcagcttg tggataaggc gcgatttttt
     8761 ccagtggctg gacgctctct gccgcccagg cggaagtggt ggcgaaagcg gcaaacaata
     8821 ctgcaggtag aatggtcttc attttttgct ttccttcttc atttataggt ttgcaccgat
     8881 gatgtcg
//
LOCUS       DAZUCE010000202         8842 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZUCE010000202 DAZUCE010000000
VERSION     DAZUCE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.202"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            425..1402
                     /gene="glaH"
                     /locus_tag="ACHY03_004028"
     CDS             425..1402
                     /gene="glaH"
                     /locus_tag="ACHY03_004028"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFS3907206.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLAQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1422..2690
                     /gene="lhgO"
                     /locus_tag="ACHY03_004029"
     CDS             1422..2690
                     /gene="lhgO"
                     /locus_tag="ACHY03_004029"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFS3907207.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2713..4161
                     /gene="gabD"
                     /locus_tag="ACHY03_004030"
     CDS             2713..4161
                     /gene="gabD"
                     /locus_tag="ACHY03_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFS3907208.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4175..5455
                     /gene="gabT"
                     /locus_tag="ACHY03_004031"
     CDS             4175..5455
                     /gene="gabT"
                     /locus_tag="ACHY03_004031"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFS3907209.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAEKHTEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5964..7364
                     /gene="gabP"
                     /locus_tag="ACHY03_004032"
     CDS             5964..7364
                     /gene="gabP"
                     /locus_tag="ACHY03_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFS3907210.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7385..8047
                     /gene="csiR"
                     /locus_tag="ACHY03_004033"
     CDS             7385..8047
                     /gene="csiR"
                     /locus_tag="ACHY03_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFS3907211.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAEQLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(8048..8497)
                     /gene="kbp"
                     /locus_tag="ACHY03_004034"
     CDS             complement(8048..8497)
                     /gene="kbp"
                     /locus_tag="ACHY03_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFS3907212.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQLYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8581..8739)
                     /gene="yqaE"
                     /locus_tag="ACHY03_004035"
     CDS             complement(8581..8739)
                     /gene="yqaE"
                     /locus_tag="ACHY03_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFS3907213.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1975 a         2299 c         2485 g         2083 t
ORIGIN      
        1 aggcggaact ctaaatatca atattcctgg gcgtagcgct cttcttttag ggaaaacagg
       61 agaaccgccg aactatctct atttgtaatt taatgttata tctgccccgc taaaacgggg
      121 cagataatat gtttagttta ctagcggtca ttttgcagtg aagccattta ccgtttttta
      181 tcgaccagat aatctgttct ctgatggtaa tcccccccta acctgttgct ttagttattc
      241 atttcctgtc tcactttgcc ttaataccct acgttaaatg ttactaattt gttgtttttg
      301 atcacaataa gaaaacaata tgtcgcattt gtgcgcattt ttcagaaatg tagatatttt
      361 tagattatgg ctacgaaatg agcatcgcca tgtcacccta catatcataa gaggatcgct
      421 tctgatgaat gcactgaccg ccgtacaaaa caacgctgtc gattcaggcc aggactatag
      481 cggattcact ctcatcccgt cggcgcaatc cccgcgtctg ctggaactca cctttaccga
      541 acagacgacc aaacagttcc tcgcgcaggt tgccgagtgg cccgtgcagg cgctggagta
      601 caaatcgttt ctgcgttttc gggtaggcaa aattcttgat gatctgtgtg cgaatcagct
      661 gcaaccgttg ctgttgaaga ctctgctaaa ccgcgctgaa ggtgcgttgt tgatcaatgc
      721 agtgggggtc gatgatgtga agcaagcgga tgagatggtg aagctggcaa cggcggtggc
      781 gcatctgatt ggtcgctcca atttcgacgc gatgagcggt cagtattacg cgcgttttgt
      841 agtgaaaaat gtcgataact cagacagcta tctgcgtcag ccgcaccgcg taatggagct
      901 gcataacgac ggcacctacg tggaagagat caccgactat gtgctgatga tgaaaatcga
      961 cgagcagaac atgcagggcg gaaattcgct gctgctgcat ctcgatgact gggaacatct
     1021 cgaccactat ttccgccacc cgctggcgcg ccgcccaatg cgctttgccg cgccgccgag
     1081 taaaaacgtc agcaaagatg ttttccatcc ggtgttcgac gttgatcaac agggtcgccc
     1141 ggtgatgcgt tatatcgacc aattcgtcca gccaaaagat tttgaagaag gcgtgtggtt
     1201 gagcgagctt tcggacgcca ttgaaaccag taaaggcatt ctttctgtac ccgttcccgt
     1261 tggcaaattc ctgttgatta acaacctgtt ctggctgcac ggtcgcgacc gctttactcc
     1321 gcacccggat ctgcgccgtg aactgatgcg tcagcgtggc tatttcgctt acgccactca
     1381 ccactaccag acgcatcagt aagcgcaaag gaattgagcg gatgtatgat tttgtgatta
     1441 ttggcggcgg catcatcggc atgtcgaccg ccatgcaact gattgatgtc tatccggacg
     1501 cacgcattgc gttgctggaa aaagagtccg gcccggcctg tcaccaaacg ggccacaaca
     1561 gtggcgtgat ccatgccggg gtttattaca cgcccggtag cctgaaggca cagttttgcc
     1621 tggcgggaaa ccgcgccact aaagcctttt gcgatcaaaa cggcattcgc tatgacaact
     1681 gcggcaagat gctggtcgcc acctctgaac tcgaaatgga acggatgcgc gcgttgtggg
     1741 aacgcacggc ggcaaacggt atcgagcgcg agtggttaaa cgccgtggaa ctgcgcgagc
     1801 gcgaaccgaa tatcaccggg cttggcggca tttttgtgcc gtccagcggc attgtcagct
     1861 accgcgaagt cacagcggcg atggcaaaaa ttttccaggc cagaggcggt gagattatct
     1921 ataacgccga agtcagcggc cttagtgagc ataaaaacgg cgtggtgata cgtacccgtc
     1981 agggcggtga atatgaagca tcgacgctga ttagctgttc cgggctgatg gctgaccggc
     2041 tggtgaaaat gctcggcctc gaaccgggct ttatcatctg cccgttccgc ggcgagtatt
     2101 tccgcctggc gccggagcat aaccagattg ttaaccatct gatttacccc attcccgatc
     2161 cggcaatgcc gtttttgggc gttcatctca cccgcatgat cgacggtagc gtcaccgttg
     2221 ggccaaacgc agtgctggcg ttcaaacgcg aaggctatcg caagcgcgac ttctcattta
     2281 gcgacacgct ggagatttta ggctcgtcgg ggattcgccg ggtgctgcaa aaccatctac
     2341 gctcaggact gggcgagatg aaaaactcgc tgtgcaaaag cggctatctg cggctggtgc
     2401 aaaagtattg tccccggctt tcgttaagcg atctccagcc ctggcccgcc ggtgtgcggg
     2461 cgcaggcggt atcgccggac ggcaagctga ttgacgattt tctgtttgtc accaccccgc
     2521 gcacgatcca cacctgcaat gcgccctccc cggcagcgac atcagcaatt cctattggtg
     2581 cgcatattgt cagcaaggta caaacgctgt tggcaagcca gagtaacccc ggacgcacgc
     2641 tgcgagcggc acgtagtgtg gatgccttac acgccgcatt taatcaataa cctttgaaaa
     2701 caggatgtag cgatgaaact gaacgacagt aacttattcc gccagcaggc gttgattaac
     2761 ggggaatggc tggacgccaa caatggtgaa gtcatcgacg tcaccaatcc ggcgaacggc
     2821 gacaagctgg gtagcgtgcc gaaaatgggc gctgatgaaa ctcgcgccgc tatcgacgcc
     2881 gccaaccgcg ccctgcccgc ctggcgtgcg ctcaccgcca aagaacgcgc caacattctg
     2941 cgcaactggt tcaatttgat gatggagcat caggacgatt tagcgcgcct gatgaccctc
     3001 gaacagggca aaccactggc tgaagcgaaa ggtgaaatca gctacgccgc ctcctttatt
     3061 gagtggtttg ccgaagaagg caaacgcatt tatggcgaca ccattcccgg tcatcaggcc
     3121 gataaacgcc tgattgttat caagcagccg attggcgtca ccgcggctat cacgccgtgg
     3181 aacttcccgg cggcgatgat tacccgtaaa gccggcccgg cgctggcggc tggctgcacg
     3241 atggtgctga agcccgccag tcagacaccg ttctctgcgt tggcgctggc agagctggcg
     3301 atccgcgcgg gcattccggc tggcgtgttt aacgtggtca ccggttcggc gggcgcggtg
     3361 ggtaatgaac tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg ctcgaccgaa
     3421 attggccgcc agttaatgga acagtgcgcg aaagacatca aaaaagtgtc gctggagctg
     3481 ggcggcaacg cgccgtttat cgtctttgac gatgccgatc tcgataaagc cgtggaaggc
     3541 gcactggcct cgaaattccg caacgccggg caaacctgcg tctgcgccaa ccgtttatac
     3601 gtgcaggacg gcgtgtatga ccgctttgcc gaaaaattgc agcaggcggt gagcaaactg
     3661 cacatcggtg acgggctgga gaaaggcgtc actatcgggc cgctgattga tgaaaaagca
     3721 gtagcaaaag tagaagagca tattgccgat gcgctggaga aaggcgcacg cgtggtttgc
     3781 ggtggtaaag cacacgaacg cggcggtaac ttcttccagc cgaccattct ggtggacgtt
     3841 ccagccaacg ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc gctgttccgt
     3901 tttaaagatg aagccgatgt gatcgcgcaa gccaatgaca ccgagtttgg tcttgccgcc
     3961 tatttctacg cccgtgattt aagccgcgtc ttccgcgtgg gcgaagcgct ggagtacggc
     4021 atcgtcggca tcaataccgg gattatttcc aatgaagtgg ccccgttcgg cggcataaaa
     4081 gcctcggggc tgggtcgtga aggttcgaag tatggcatcg aagattactt agaaatcaaa
     4141 tatatgtgca tcggtcttta actggagaat gcgaatgagc agcaataaag agttaatgca
     4201 gcgccgcagt caggcgattc cccgtggcgt tgggcaaatt caccccattt tcgctgatcg
     4261 cgcggaaaac tgccgggtgt gggacgtaga aggccgtgag tatcttgatt tcgcgggcgg
     4321 cattgcggtg ctcaataccg ggcacctgca tccgaaagtg gttgcggcgg tggaagcgca
     4381 gttgaaaaaa ctgtcgcaca cctgcttcca ggtgctggct tacgagccgt atctggagct
     4441 gtgcgagatt atgaatcaga aggtgccggg cgattttgcc aagaaaacgc tgctggttac
     4501 gaccggttcc gaagcggtgg aaaacgcggt gaagatcgcc cgcgctgcca ccaaacgtag
     4561 cggcaccatc gcctttagcg gcgcgtatca cgggcgcacg cactacacgc tggcgctgac
     4621 cggtaaggtg aatccgtact ctgcgggcat gggcctgatg ccgggccatg tttatcgcgc
     4681 actttatcct tgcccgctgc acggcatcag tgaagatgat gctatcgcca gtatccaccg
     4741 aatttttaaa aatgatgcgg cgccggaaga tattgccgcc atcgtgattg agccggttca
     4801 gggcgaaggc ggtttctacg ccgctacgcc agcttttatg cagcgtttac gcgcgctgtg
     4861 tgatgagcac gggatcatgc tgattgccga tgaagtgcag agcggcgcgg ggcgtaccgg
     4921 cacgctgttt gcgatggagc aaatgggcgt ggcaccagat ctcaccacct ttgcgaaatc
     4981 gatcgcaggc ggcttcccgc tggcgggcgt caccgggcgc gccgaagtga tggatgccgt
     5041 cgctccaggc gggctgggcg gcacctatgc cggtaatccg attgcctgcg tggcggcgct
     5101 ggaagtgttg aaggtgttcg agcaggaaaa tctgctgcaa aaagccaacg ttctggggca
     5161 gaagctgaaa gacggattgt tggcgatcgc cgaaaaacac acggagatcg gcgacgtacg
     5221 cggtctgggg gcgatgatcg ccatcgagct gtttgaagat ggcgatcaca gcaaaccgga
     5281 cgccaaactc accgccgaga tcgtagctcg cgcccgcgat aagggcctga ttcttctctc
     5341 ctgcggcccg tattacaacg tgctgcgcat cctggtaccg ctcaccattg aagatgctca
     5401 gattcgtcag ggtctggaga tcatcagcca gtgttttgct gaggcgaagc agtagcaccg
     5461 cccccatgcc ggaggcgacg cttacgcgtc ttgtccggcc tacgagagtg taggtcggat
     5521 aaggcgttaa cgccgcatcc gacattcggc gcacccatgc cggaggcgac gctgcgcgtc
     5581 ttgtccggcc tacggggatc caggtcggat aaggtgttta cgccgcatcc gacattcggt
     5641 gcaccatgcc ggaggcgacg cttacgcgtc ttgtccggcc tacggaaatg taggtcggat
     5701 aaggcgtttg cgccgcatcc gacaatcggc gcacccatgc cggaggcgac gctttgcgtc
     5761 ttgtccggcc tacggggatt ctcgtcggat aaggcgttta cgccgcatcc gacaatctgt
     5821 acgtgaacag gaagaaatct atgttggccg ggtaaggcgg agccgctctc cggcaaaaag
     5881 aatcaataac aattatacgc gtgacccgac gcgggaaatg tcggggcgct ctcccaagtg
     5941 acacactttc gagaggattc aggatggggc aatcatcgca accacatgag ttaggcggcg
     6001 ggctgaagtc acgccatgtc accatgttgt ctattgccgg tgttatcggc gcaagtctgt
     6061 ttgtcggttc cagcgtcgcc atcgccgaag cgggcccggc ggtattactg gcctatctgt
     6121 tcgccgggct actggtggtt atgattatgc ggatgttggc ggaaatggcg gttgccacgc
     6181 ccgataccgg ttcgttttcc acctatgccg ataaagctat tggtcgttgg gcgggttata
     6241 ccatcggctg gttgtactgg tggttttggg tactggttat cccgctggaa gccaacatcg
     6301 ccgccatgat cctgcactca tgggttccag gcattcccat ctggttattt tccctcgtca
     6361 ttacccttgc cttaactggc agtaatttat taagcgttaa aaactacggc gaatttgagt
     6421 tctggctggc gctgtgcaaa gtcatcgcta tcctggcctt tattttcctt ggtgcagtcg
     6481 caattagcgg tttttacccg tatgccgacg tgagcgggat ctcaagattg tgggatagcg
     6541 gcggctttat gcccaacggc ttcggtgcgg tattaagcgc gatgttgatc accatgttct
     6601 cgtttatggg cgcagaaatt gtcaccattg ccgccgcgga atccgacacg ccggaaaaac
     6661 atattgtccg cgctaccaac tcggttatct ggcgtatttc tatcttctat ttgtgctcta
     6721 tttttgtcgt agtggcgtta ataccgtgga atatgccggg gctgaaagcc gttggatctt
     6781 atcgctcggt tctggaattg ctcaatatac cccatgcgaa attaatcatg gactgcgtga
     6841 tattactttc cgtaaccagc tgtctgaact cggcgctgta taccgcgtca aggatgctct
     6901 actccttaag ccgtcgcggt gatgcgcccg cggtaatggg caaaatcaac cgcagtaaaa
     6961 ccccgtatgt ggcggtgtta ctctccaccg gagcggcatt tttaacggtg gtggtgaact
     7021 attacgcacc tgcgaaagtg tttaaattcc tgatcgacag ctccggtgct atcgccctgc
     7081 tggtttattt agtcatcgcc gtttcacagt tgcggatgcg taaaattctg cgagcagaag
     7141 gaagcgaaat tcgcttgcgc atgtggcttt acccgtggct cacctggctg gtcatcggct
     7201 ttattacctt tgtgttggtg gtgatgttat tccgcccggc gcaacagtta gaagtgatct
     7261 ctaccggctt attagcgata gggattatct gtaccgtgcc gattatggcg cgctggaaaa
     7321 agctggtatt gtggcaaaaa acacccgttc ataatacgcg ctgataatga tcaggagtca
     7381 caccatgacc attacgtctc tggatggcta tcgctggctg aagaacgata ttattcgcgg
     7441 taattttcaa ccggatgaaa aattacgaat gagtttgctg acatcgcgtt atgcacttgg
     7501 cgttgggccg ttacgggaag ctctttcgca actggtggcg gaacagctgg tcacggtggt
     7561 gaatcaaaaa gggtatcggg tggcgtctat gtcagagcag gagctgctcg atattttcga
     7621 cgcccgcgcc aatatggaag cgatgttagt gagtctggcg attgcccgcg gtggcgatga
     7681 gtgggaggca gacgttctcg caaaagcgca tctgctgagt aagcttgagg cctgtgacgc
     7741 cagcgagaaa atgcttgatg agtgggatct gcgtcatcag gcgtttcata cggcaattgt
     7801 ggcgggctgt ggttctcact atttgctgca aatgcgtgaa cggttgtttg atctggcggc
     7861 gcgttatcga tttatctggc tgcggcgaac ggtgctttcg gtggaaatgc tggaggataa
     7921 acacgatcag caccagaccc tgactgcggc ggtactggcg cgagataccg cgcgcgccag
     7981 tgagttaatg cgccagcatt tactgacgcc aattcccatt atccagcagg cgatggctgg
     8041 caattaacta ctcttccgga atacgcaaca cttgccccgg ataaatttta tccgggcttt
     8101 ttagcatcgg tttattcgct tcgaagattt tattgtacag attagcgtta ccgtagacct
     8161 gtttggaaat ggcactcaga gtgtcgccag acttaacggt ataaagctgg ctggcagtgg
     8221 ctggtgtcgc cgttttcacc tgatcatcaa cactggcaat gccggaaata ttccccaccg
     8281 caacaaggat tttctctttc gcctcctgac taagaccgtc accagtgacc gtcgctttgc
     8341 cgtcggcaat ttgaatattc actttatcgg catcaggtat accggttttg ttcagatgct
     8401 cctgcacctt cttcgcctga tcgtctttat cgtgctgacc tgtaaccgcg tcccagagtt
     8461 tttctccggc atctttcaca aaattgaaca gacccatagc tacctcattc gttaacggaa
     8521 acactcagaa agtgtagcag aggaacgcgg cgcgttaacg ccgcgggggt gagcacaggt
     8581 ttaatcgcgg gtttgcaccc agaacgcgtg aatcaaaccg gggatatacc caagcagtgt
     8641 cagcagaata ttaataatga acgcccaacc gaacccttta ccgagcagca cgccgagcgg
     8701 cggcagaatg atggtgatga cgattctcca gaaacccata tgtactccct ataagaaaat
     8761 tactcattgt ttaaaaagag attttatctc ttaagcgtag taattttagc ggagcctgcc
     8821 agtttcagct tacgctttta ct
//
LOCUS       DAZUCE010000203         8797 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZUCE010000203 DAZUCE010000000
VERSION     DAZUCE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.203"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..81)
                     /gene="timP"
                     /locus_tag="ACHY03_004036"
     CDS             complement(<1..81)
                     /gene="timP"
                     /locus_tag="ACHY03_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFS3907214.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNLSLS"
     gene            complement(441..1286)
                     /gene="sseA"
                     /locus_tag="ACHY03_004037"
     CDS             complement(441..1286)
                     /gene="sseA"
                     /locus_tag="ACHY03_004037"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFS3907215.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVALEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            1493..6454
                     /gene="yfhM"
                     /locus_tag="ACHY03_004038"
     CDS             1493..6454
                     /gene="yfhM"
                     /locus_tag="ACHY03_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFS3907216.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDLPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSTEPLTGEKAQNSNLNSDQLATLQVTNSGEQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            6455..8767
                     /gene="pbpC"
                     /locus_tag="ACHY03_004039"
     CDS             6455..8767
                     /gene="pbpC"
                     /locus_tag="ACHY03_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFS3907217.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGTLPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIASVHFSFQ"
BASE COUNT         2079 a         2222 c         2464 g         2032 t
ORIGIN      
        1 ggagagactc aggttattat ttacctcggt taacagcgca ccagaaacaa taagtacgat
       61 acagaagcac cgtatcttca ttgtgcggtc catgcgaaga ccgcatcacg acagcaccac
      121 tggcgatatt gccgcgatag caagcactgt ggggtgcctg tatttacggt catggaacat
      181 cctttcctaa agccagcgcc cgtttgcgag acggcgaaaa gggccattaa gaataatact
      241 cttatgtttg aataattact ctgtttatta tcttgacaca gccaggcata aaagagagta
      301 tttcaacact gtggggtgcc tgcgttgctc cggcaacaca agtcaacctg ctggaaaggc
      361 cgcgaaccag accagcaata aaaaaccgcc aaattcggcg gttttttatt ttcggcggtt
      421 ttttattgcc tgtaaaaagc ttatttcaat ggctcaaccg gtaaatctgc ccgcgcgccc
      481 cattcactcc atgcgccgtc gtacagtttc acgtttggca catccagcgt cgcgagtgcc
      541 aacaaaacca cggctgctgt tacaccagag ccgcagctga cgataatcgg tttgtcatag
      601 ctgacgccgc gaccaaaaaa tatcgcatcc agttcatcgg tcgtttttag ttcgccttcg
      661 cgcaccagtt ccgtccacgg aacgttcaat gcaccgggaa tatgtccgcg acgtaaacct
      721 gggcgaggtt catcaacttc tgcgttaaaa cgtgcagccg ggcgggcatc aataatttgc
      781 gccgtatttt catggcttgc caataacaca tcggttactt tcaccactgc ttcaggatta
      841 aacgcggcgt taaactctcc ttccggcagc tctactgcac cttcttctaa caacagatca
      901 tcgcgctgcc agcctgcaag tccacccccc agaatcgaca ctttctctac accaaaggtg
      961 cgcagcatcc accatgctcg tggggctgag aaaagattac cttcgtcata gacaatcagg
     1021 tgcttatcct ggttaacgcc taattcacgc atcgccacgg cgaacgtttc cgggcgcggc
     1081 agcatgtgcg gaagcgggga agtgtgatca gaaagcgctt cgatatcaaa aaacactgcg
     1141 ccgggaatat gtccattcag atactccaga gcaacgttac gatcctcctg tccaggcgac
     1201 gccatgcggg catcgataat ctgaatttcc gggtcatcaa tatgttcggc gagccagtcg
     1261 gctcctacaa accatgtcgt ggacataggc atctccattt ttgccggggt taggctaaat
     1321 tgtcggatgt ttagtgaaaa gcgacaagca aaagaggctg atttggctgt ctgagccaca
     1381 attctcacta aaattcggat tgtatgggcg tgatactgaa ttacataacg taaattctca
     1441 cgcataataa ttattcgacg ttgctaacgg gccattgtgg cagggatgaa aaatgaaaaa
     1501 gttacgcgta gccgcctgca tgctaatgct ggcgctggca gggtgcgaca acaacgataa
     1561 cgcgccaaca gcggtgaaaa aagatgcgcc ttctgaagtt actaaagcgg cctcttcaga
     1621 aaacgcgagt tcagcaaaac tctccgtgcc ggagcgacaa aaactggccc aacagagtgc
     1681 cggtaaggtg ctgacattgc tggatctctc tgaagtccaa cttgatggtg cagccacgct
     1741 ggtgctgacg ttctccatcc ctctcgaccc ggatcaggat ttctcacgcg ttattcatgt
     1801 cgtcgataaa aaaagcggca aagtggatgg tgcctgggag ctgtcagata atcttaaaga
     1861 gctgcgttta cgccacctcg aaccgaaacg tgatttgatc gttactattg gcaaggaggt
     1921 caaagcactc aacaacgcaa ccttcagtaa agattacgaa aaaactataa ctacccgcga
     1981 catccaaccc agcgtcggtt ttgccagccg tggttcgctg ctgcctggta aagtcgttga
     2041 agggctgccg gtaatggcgc tcaacgttaa taatgtcgat gttaacttct tccgcgttaa
     2101 gccagaatct ctgccagcat tcattagcca atgggaatac cgcaattcgc tggcgaactg
     2161 gcagtcagac aaactgctgc agatggcgga tctggtctac accggacggt ttgatctcaa
     2221 tcctgcgcgt aacacccgtg aaaaattatt gctgccgctg ggcgatatca aaccgcttca
     2281 gcaggcgggc gtgtatctgg ctgtgatgaa tcaggctgga cgttacgatt acagtaatcc
     2341 cgcgacgctg tttacgttaa gtgatatcgg cgtttcagct caccgttatc acaatcgtct
     2401 ggatatcttt acccaaagtc tggaaaacgg cgcggcccag caaggaattg aagtctcttt
     2461 attaaatgag aaagggcaga ctctgactca ggcaaccagt gacgctcagg ggcatgtgca
     2521 gctggaaaat gataaaaacg ccgcattatt gctggcacgt aaagacggtc agacaacgct
     2581 actcgattta aaacttccgg cgctggactt agcagaattt aacattgctg gcgcgccagg
     2641 ctatagcaaa cagtttttca tgtttggccc gcgcgatctt tatcgcccgg gtgaaacggt
     2701 aatcctcaat ggtttgctgc gtgatgcaga cggtaaagcg ttgcccaatc aacccatcaa
     2761 gttagacgtg attaaacccg atgggcaggt actcaggagc gtcgttagtc agccggaaaa
     2821 tggcctctac cactttacct ggccactcga tagcaatgcg gcaaccggta tgtggcatat
     2881 tcgcgctaac acgggcgata accagtatcg gatgtgggat ttccacgtcg aagattttat
     2941 gccagagcgt atggcgctga atctgaccgg tgagaaaacc ccgcttacgc cgaatgatga
     3001 agtgaaattc tcggtggtgg gatactacct gtacggtgcg cctgctaatg gtaatacttt
     3061 gcaagggcaa cttttcctgc gcccactgcg tgaggctgtg tcagccttac ctggttttga
     3121 attcggcgat atagctgccg aaaacctttc ccgcacgctg gatgaagttc agttgacgct
     3181 ggatgataaa gggcgcggcg aagtttctac agaaagccag tggaaggaaa cgcattcccc
     3241 attacaggtt attttccagg gtagtttgct ggaatcgggc ggtcgcccag tgacgcgccg
     3301 cgctgagcag gctatctggc ctgccgatgc attgccgggg ctccgtccgc agttcgcctc
     3361 gaaatcggtt tacgattatc gcactgacag cacggtgaaa cagcccattg ttgatgaagg
     3421 cagtaacgcc gcttttgaca tcgtttatag cgatgcgcaa ggcgtgaaaa aagccgtgtc
     3481 gggcttgcag gtgcgcctga ttcgcgaacg ccgcgattac tactggaact ggtcagaaga
     3541 tgaaggctgg caatcacagt ttgatcaaaa agatctgatt gaaaatgaac aaactctgga
     3601 tctgaaagcg gacgaaaccg gcaaggtcag tttcccggta gagtggggcg cttatcgtct
     3661 ggaagtcaaa gcgccgaatg aagcggtcag tagtgtgcgt ttctgggctg gctatagctg
     3721 gcaggacaac agcgacggga gcggtgccgt gcgacccgac cgtgtcacgc tgaaactaga
     3781 taaagccagt tatcgccctg gcgataccat taagttgcat attgccgcgc caacggcggg
     3841 taaaggttat gcgatggtcg agtccagtga agggccgctg tggtggcaag agattgatgt
     3901 tccggctcaa gggctggatc tgacgattcc ggtcgataaa acctggaatc gtcatgatct
     3961 gtatttaagt acgctggtag tacgtcctgg cgataaatct cgctccgcga cgccaaaacg
     4021 cgcggttggt gtgttgcatc tgccgcttgg tgatgaaaac cgtcgcctcg atctggcgct
     4081 ggaaacacca gcaaaaatgc gtcccaatca accattaacc gtgaaaatta aagccagcac
     4141 taaaaatggc gagaagccta aacaggtgaa tgtgctggtg tctgccgttg atagtggtgt
     4201 gctgaatatt actgactacg tcacgccaga tccgtggcag gcgttctttg gtcagaaacg
     4261 ctatggcgca gacatttacg atatttacgg tcaggttatt gaaggtcagg ggcgtctggc
     4321 agctctgcgt ttcggtggcg atggtgatga gctgaaacgt ggtggtaaac cgccggtcaa
     4381 tcacgtcaat attgtcgcgc agcaggcgct gccggtaacg ctcaacgaac agggcgaagg
     4441 ctcggttaca ctgccgattg gcgattttaa cggtgaattg cgcgtcatgg cgcaagcctg
     4501 gacggcagat gacttcggta gcaacgaaag caaagtgata gttgccgcac cggtgattgc
     4561 tgaactgaac atgccgcgct ttatggcgag tggcgatacc tcgcgtctga cgctggatat
     4621 cactaatctt accgataaac cgcaaaaact gaacgttgcc ctgaccgcca gtggtttgct
     4681 tgaactggtc agcgatttac ccgcagccgt tgaattagcg ccaggtgtgc gtactacgct
     4741 gtttatcccg gtgcgagcat tgccgggtta tggcgatgga gaaattcagg ccaccattag
     4801 cgggttagcg ttaccgggtg aaaccgttgc cgatcagcat aagcagtgga aaatcggcgt
     4861 ccgtccggcg ttcccggcac aaacggttaa ttacggtacg gcgttacagc ctggtgagac
     4921 atgggcgatt ccggcggatg gattgcaaaa cttctcgcct gttacgctgg aagggcaatt
     4981 gttgttgagc ggcaaaccac cgctgaacat cgcacgttat atcaaagagt taaaagcgta
     5041 tccgtacggc tgtcttgagc aaaccgccag cggcctgttc ccgtcacttt ataccaacgc
     5101 agcccaactg caggcgttgg gcatcaaagg cgacagtgat gagaaacgcc gtgcatcggt
     5161 cgatatcggc atttcccgtt tgctgcaaat gcaacgtgat aacggcggct ttgcgctgtg
     5221 ggataaaaac ggtgacgaag agtactggct gacggcttac gtgatggatt tcctggtccg
     5281 cgcaggcgaa cagggttaca gcgtgccgac agacgccatt aaccggggta atgagcgtct
     5341 gctgcgctat ttacaagatc cgggcatgat gtcgatcccg tacgcggata atctcaaagc
     5401 cagtaaattc gccgtacagt catacgctgc gctggtgttg gcccgtcagc aaaaggctcc
     5461 gctgggtgcg ctgcgtgaaa tctgggagca tcgtgcagat gccgcttctg gtttaccgct
     5521 gctgcaactt ggcgttgcgc tgaaaaccat gggtgatgcg acgcgtggtg aagaagcgat
     5581 tgcgctggcg ctaaaaacgc cgcgtaatag tgatgagcgg atatggctgg gtgattacgg
     5641 tagtccactg cgcgacaacg cgttgatgct ctccttgctg gaagagaaca aactgctacc
     5701 cgatgagcag tactccctgc tgaacacact ttcgcagcag gcgtttggtg aacgctggct
     5761 atcgacgcag gaaagtaacg cgttgttcct ggctgcccgt acgattcagg atttaccagg
     5821 taaatggcag gcgcaaacct ctttctcaac tgagccgctg acaggcgaga aagcgcaaaa
     5881 cagcaatctg aatagcgatc aacttgccac cttgcaggtg accaacagtg gcgagcagcc
     5941 gttatggctg cgtgtggatg ccagcggtta tccgcaatcc gcacctttac cggcgaacaa
     6001 tgtgctgcaa attgagcgtc atattcttgg tactgatggt aagagcaaat cgctggactc
     6061 gttacgtagc ggcgatctgg tgctggtctg gttgcaggta aaagccagta acagcgtgcc
     6121 ggatgcgtta gtcgtggatc tgctgcctgc gggtctggaa ctggaaaacc agaatctggc
     6181 gaacggtagc gccagcctgg agcaaagtgg tggcgaagtg cagaacttac tgaaccagat
     6241 gcagcaggcg agtattaagc acattgagtt ccgtgacgat cgctttgtgg cggcggttgc
     6301 cgttgatgaa taccaaccgg taacgctggt gtatctggcg cgggcggtga cgccgggaac
     6361 gtatcaggta ccgcaaccga tggtggaatc aatgtatgtt ccccaatggc gggcgaccgg
     6421 cgcggctgaa gatttgctga ttgtcagacc gtaaatgcct cgcttgttaa ccaaacgcgg
     6481 ctgctggata acgctggcag ctgcgccatt tctccttttt cttgcagcgt ggggagcaga
     6541 taaactctgg cctctaccgc tgcatgaagt caatcccgca cgagtggtcg tggcgcagga
     6601 tggtacgccg ctctggcgct tcgccgatgc tgacggcatc tggcgttatc cggtaacaat
     6661 cgaagatgtt tctccacgtt accttgaagc gctgatcaat tatgaagatc gctggttctg
     6721 gaagcatccg ggggtgaatc cattctcggt ggcgcgcgca gcatggcaag atctcacttc
     6781 gggacgggtt atttccggtg gcagcacgct cactatgcag gttgctcgtc tgcttgatcc
     6841 tcaccccaaa acatttggcg gcaaaattcg ccagctctgg cgcgcgttgc aactggaatg
     6901 gcatctgtct aagcgtgaaa ttctgacctt gtatcttaac cgcgctccgt ttggcggtac
     6961 gttgcagggg atcggtgcgg caagttgggc ttatctcgga aaatcgcctg cgaatttaag
     7021 ctattccgag gcggcaatgc tggcggtgtt accgcaagcg cccagccgac tgcgcccgga
     7081 tcgttggccg gagcgtgccg aagccgcgcg taataaagtg ctcgaacgga tggccgtgca
     7141 aggtgtgtgg tcccgtgagc aggtaaaaga gtcaagggaa gaacccatct ggctggcacc
     7201 ccgacaaatg ccgcaacttg caccgctgtt ttcgcgcatg atgctcggta aaagcaaaag
     7261 cgacaaaatc gttactacgc tggatgccgg tcttcaacga cgtctggaag aactggcgca
     7321 aaactggaaa ggacgtttac caccgcgcag ctcactggcg atgatcgtgg ttgatcatac
     7381 cgatatgcgt gttcgcggct gggtgggttc ggttgatctc aacgatgatt cacgctttgg
     7441 tcatgttgat atggtcaatg cgatccgatc gccaggatcg gtgctcaaac cgtttgttta
     7501 cggtctggcg ctggatgaag gattgatcca cccggcatca ctgctgcaag acgtcccccg
     7561 gcgtaccggt gattaccgac ctggcaactt tgacagcggt tttcatggcc cgatcagcat
     7621 gagcgaggcg ctggtgcgct cgctgaattt acctgctgtg caggtgctgg aagcctatgg
     7681 accgaaacgg tttgcggcaa agttacgcaa tgttggattg ccgttatatt tgcccaacgg
     7741 tgctgcgccg aatctttcgc tcattctcgg cggcgctggt gcgaaactgg aagatatggc
     7801 ggcagcgtat accgcgtttg ctcgccacgg caaggcaggc aaattgcgct tacagcctga
     7861 cgatccgctg cttgaacggc ctttaatgtc gtcgggggcg gcgtggatca ttcgccggat
     7921 tatggctgat gaagcgcaac ccttgccgga tggtaccttg ccgcgcgtcg ccccactggc
     7981 atggaaaacg ggcaccagct atggctatcg tgacgcctgg gcgattgggg ttaatgctcg
     8041 ctatgtcatt gggatctgga ctggcagacc ggatggcacg ccggttgttg gtcagtttgg
     8101 ctttgccagt gctgtaccgc tgttgaatca ggtcaataat attttactgt cgcgcagtgc
     8161 gaatctgccg gaagacccgc gcccgaactc agtcactcgt ggcgttatct gctggccggg
     8221 cggacagtct ttgccggaag gtgacggtaa ctgtcgccgc cgcctggcaa cctggttgct
     8281 ggacgggagc cagccgccaa ctctattact gccggagcag gaaggcatta atggcattcg
     8341 tttccccatc tggctggatg aaaatggcaa acgtgttgcc gctgattgcc cgcaagcgcg
     8401 acaagaaatg ataaatgtct ggccgctacc gctggaaccc tggttgcccg catcagaacg
     8461 tcgcgctgtg cgcttaccac cggcctcgac aatttgcccg ccatacggtc acgatgccca
     8521 gctcccgctg cagctgacag gcgttcgcga tggcgagatt attaaacgtt taccgggcgc
     8581 agcggaagcg actttgccgt tgcaatcaag tggaggggca ggtgaacgct ggtggtttct
     8641 gaatggcgaa ccgttaactg aacgcgggcg caacgtgacc ctgcatttga cggataaagg
     8701 tgattatcaa ttgctggtga tggatgacgt agggcaaatc gcgtcagtac atttttcatt
     8761 tcagtgaaat atacaaaata tttattaatc taattat
//
LOCUS       DAZUCE010000204         8787 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZUCE010000204 DAZUCE010000000
VERSION     DAZUCE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.204"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(236..312)
                     /locus_tag="ACHY03_004040"
     tRNA            complement(236..312)
                     /locus_tag="ACHY03_004040"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(276..278),aa:Asp,seq:gtc)
     gene            complement(445..1176)
                     /gene="dnaQ"
                     /locus_tag="ACHY03_004041"
     CDS             complement(445..1176)
                     /gene="dnaQ"
                     /locus_tag="ACHY03_004041"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFS3907218.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1241..1708
                     /gene="rnhA"
                     /locus_tag="ACHY03_004042"
     CDS             1241..1708
                     /gene="rnhA"
                     /locus_tag="ACHY03_004042"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFS3907219.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1705..2427)
                     /locus_tag="ACHY03_004043"
     CDS             complement(1705..2427)
                     /locus_tag="ACHY03_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS3907220.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2461..3216
                     /gene="gloB"
                     /locus_tag="ACHY03_004044"
     CDS             2461..3216
                     /gene="gloB"
                     /locus_tag="ACHY03_004044"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFS3907221.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPDERFAWLRSKKDRF"
     gene            3288..4646
                     /gene="mltD"
                     /locus_tag="ACHY03_004045"
     CDS             3288..4646
                     /gene="mltD"
                     /locus_tag="ACHY03_004045"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFS3907222.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4694..5464)
                     /locus_tag="ACHY03_004046"
     CDS             complement(4694..5464)
                     /locus_tag="ACHY03_004046"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS3907223.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVDAHKAA"
     gene            complement(5542..6342)
                     /locus_tag="ACHY03_004047"
     CDS             complement(5542..6342)
                     /locus_tag="ACHY03_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS3907224.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6583..7497
                     /gene="yafC"
                     /locus_tag="ACHY03_004048"
     CDS             6583..7497
                     /gene="yafC"
                     /locus_tag="ACHY03_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFS3907225.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGALTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7494..8297)
                     /gene="dkgB"
                     /locus_tag="ACHY03_004049"
     CDS             complement(7494..8297)
                     /gene="dkgB"
                     /locus_tag="ACHY03_004049"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFS3907226.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKEGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8460..8536)
                     /locus_tag="ACHY03_004050"
     tRNA            complement(8460..8536)
                     /locus_tag="ACHY03_004050"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8500..8502),aa:Asp,seq:gtc)
     gene            complement(8591..8706)
                     /gene="rrf"
                     /locus_tag="ACHY03_004051"
     rRNA            complement(8591..8706)
                     /gene="rrf"
                     /locus_tag="ACHY03_004051"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2335 a         2116 c         2123 g         2213 t
ORIGIN      
        1 aaatcaattt gaaatataca ttcatttaat caatataaat ttattctctg gaaatatatt
       61 taagctgaat ggttcgatat cattattgaa ttttaatgaa tgagacaaca agcgagaatc
      121 acgcacttac gccagagtaa aatcattgaa cagagaaatg gtggaattca aatacaaaaa
      181 aaccgcaaaa ttaaaaatct tgcggttctc tgaactcatt ttcatgagtg aatattggcg
      241 gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg
      301 aactaccgct ccgcgttgtg ttccgttggg aacgaggcga atattacgga ttgcctcgac
      361 cttcgtcaac ggtttttctc atcttttgaa tcgtttgctg caaaaatcgc ccaagtcgct
      421 atttttagcg ccttttacat gtatttatgc ccgccagagg cagcttccgc ccttcttctg
      481 caccagatca agacgggctt catgagctgc aagctcttca tctgtcgcaa aaacaacgcg
      541 taacttactt gcctgacgta caatgcgctg aattgttgct tcaccttgtt gctgttgtgt
      601 ctctccttcc atcgcaaaag ccatcgacgt ttgaccaccg gtcatcgcca gataaacttc
      661 cgcaaggatc tgggcatcga gtaatgcccc gtgcagcgtt cgtttactgt tatctatttc
      721 gtagcgggca cataacgcat cgaggctgtt gcgtttaccg ggaaacattt tcctcgccac
      781 cgcaaggcta tcggtgacct tacagaaggt attagtcttc ggaatatcgc gcttgagcaa
      841 cgagaactcg tagtccataa agccgatatc gaacgctgcg ttatggatca ccaactccgc
      901 gccgcggata tagtccatga actcatcggc tacttcggca aacgtgggct tatcgagcaa
      961 aaattcatcg gcaataccat gtacgccaaa ggcttccgga tccaccagcc gatcgggttt
     1021 gagataaaca tggaagttat tgcccgtcag gcgacggttc accacttcaa cggcaccaat
     1081 ctcaatgatc ttgtggcctt catagtgcgc accaatctgg ttcataccgg tggtttcggt
     1141 atcgagaacg atctggcgtg taattgcagt gctcatagcg gtcatttatg tcagacttgt
     1201 cgttttacag ttcgattcaa ttacaggaag tctaccagag atgcttaaac aggtagaaat
     1261 tttcaccgat ggttcgtgtc tgggtaatcc tggacctggg ggttacggcg ctattttacg
     1321 ctatcgcgga cgcgagaaaa cctttagcgc aggctacacc cgcaccacca acaaccgtat
     1381 ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa gaacattgcg aagtcatttt
     1441 gagtaccgac agccagtatg tccgccaggg tatcacccag tggatccata actggaaaaa
     1501 acgtggctgg aaaacagcag acaaaaaacc agttaaaaat gtcgatctct ggcaacgtct
     1561 tgatgctgcg ctggggcagc atcaaatcaa atgggaatgg gttaaaggcc atgccggaca
     1621 cccggaaaac gaacgctgtg atgaactagc tcgtgccgcg gcgatgaatc ccacactgga
     1681 agatacgggc taccaagtcg aagtttaagc ctgtggttta cgacattgcc gggtggctcc
     1741 aaccgcctgg cgaattcgtg gcttgttttt actctgtttc atcggattta gcgttaaagg
     1801 aatagtccgt ttccgggcaa caataagttg taagcaacca agcgcaggaa tatgcgcatt
     1861 caaaagtttt cctccgtgtt tgttccacgg gagaacgtgg aaacggctgg cgtgtagcac
     1921 ttcaaaattc aacaaagaga gccagtccag ctgccgcatc agagtaaaca tccggctgtt
     1981 atagggcgag gttttgcgca ataccggcac aagtttgcgt aatcccatta aactaatggg
     2041 attgaagcca ctaatcacca gccagccatc atcaatcaat acccgatcag cttcacgcaa
     2101 taaacgatgc ggatcggtgc accacggcaa tgtatgtgcc agtagacaaa catcaacgga
     2161 tttatcggca aaaggaagat gaagtggatc cgcctgcacc tggacgggca ttccttgcgc
     2221 agaaacattc acttgatgag aaaccgcgca cgcttcgcaa ttgatttctg cgcttaaatt
     2281 gccaatctta agcagatgaa aaccatacat tttagtgaac cacgggttga gctggcgctc
     2341 cagcgccttg cgataaagct ctccccaggg caaatcgccc cagcgatcag gagccacgac
     2401 agtttgaggg actcttgccg gtttcatcac aaccttccgt ttcacactga gaggtaatct
     2461 atgaatctta acagtattcc cgcctttgat gacaattaca tctgggtttt gaatgatgaa
     2521 gcaggtcgct gcctgattgt cgatcccgga gacgcagagc cagtattaaa cgccattgcc
     2581 gccaataact ggcaaccgga ggccatattt ctcacccacc atcatcacga tcacgttggc
     2641 ggcgtaaaag aactggtgga aaagtttccg caaattgtgg tgtatggtcc acaagagaca
     2701 caagataagg gaacaacaca ggtagtcaaa gatggcgaaa ctgccttcgt tttggggcat
     2761 gaatttagtg taattgccac gccgggtcac actttaggac atatctgtta cttcagtaaa
     2821 ccttatctat tttgcggcga cacgctgttt tctggtgggt gtggtcggtt gtttgaaggg
     2881 acagcatcac aaatgtatca atcacttaaa aagttaagtg cgttacctga cgatacattg
     2941 gtatgttgtg ctcatgaata tacgttatca aatatgaagt ttgctttgag tattcttccg
     3001 cacgatttgt ccataaatga ttattatcgt aaagttaagg agttacgggc aaaaaatcaa
     3061 ataacactac ccgtaattct gaaaaatgag cggcaaatta atgttttttt aagaacagaa
     3121 gatattgatt taattaatgt aattaatgaa gaaacattat tgcaacaacc tgatgaacgt
     3181 tttgcatggt taaggtcaaa gaaagatagg ttctgataaa actttcttgt catcggctcc
     3241 gttcgccgtt atgatcggtc gtcttttaag caactattga cacacacatg aaggcaaaag
     3301 cgatattact cgcctctgtc ctgctcgtgg gttgccagag taccggcaac gttcaacagc
     3361 acgcacagag cctttctgca gctggtcaag gggaagcagc aaagtttaca agtcaggcac
     3421 gatggatgga cgatgggacg tctatcgcgc cagatggtga cttgtgggct ttcattggcg
     3481 acgagctaaa gatgggaatt ccggaaaatg accggattcg cgaacagaaa cagaaatatt
     3541 tacgcaataa gagctatctc cacgatgtaa ctttacgggc agagccgtat atgtactgga
     3601 tagccgggca agttaaaaaa cgtaacatgc ctatggaact ggtactacta cccatagtgg
     3661 agagcgcttt tgatcctcac gcaacgtctg gcgccaatgc cgcaggcatc tggcagatca
     3721 ttccgagcac ggggcgcaat tatggtttga aacagacccg caattatgac gcgcgtcgcg
     3781 atgttgttgc ttccacaact gccgcgctga acatgatgca gcgtctgaac aagatgtttg
     3841 acggcgactg gcttctgacc gtagcggctt ataacagcgg cgaaggtcgg gtcatgaagg
     3901 caattaaaac gaacaaagcg cgtgggaaat ccacggactt ctggtcgtta cctttgccgc
     3961 aggaaacgaa gcagtacgtg cctaaaatgc tggcattgag tgatattctc aaaaacagca
     4021 agcgttatgg cgtacgtctg ccaacgaccg atgaaagccg tgctctggcg cgtgtgcacc
     4081 tgagcagccc ggttgaaatg gcgaaggttg cagatatggc ggggatttcc gtcagcaagc
     4141 tgaagacatt caacgctggc gtgaaaggct ccacgctggg cgcaagtggt ccgcaatacg
     4201 tgatggtgcc aaagaagcat gcagatcaac tgcgtgaatc tctggcttca ggcgaaattg
     4261 ctgctgtaca gtcgacgctg gttgccgaca atacgccgct taacagccgt gtttacaccg
     4321 tacgctctgg cgacacgctt tcaagtatcg cttcacgtct cggcgtaagc accaaagatt
     4381 tgcagcagtg gaacaaactg cgcggatcta agctgaagcc aggccaaagt ttgacgattg
     4441 gtgcaggcag tagcgcacag cgactggcaa acaacagcga tagcattacg tatcgtgtgc
     4501 gcaaaggcga ttcgctttca agcattgcta aacgccacgg cgtgaacatc aaagatgtga
     4561 tgcgctggaa cagcgacact gcgaatctgc aaccaggcga taagctgacg ttgtttgtga
     4621 aaaacaacaa catgccagat tcctgacaaa ccagataata aaaaggcacc gattcccccg
     4681 gtgccttttt tatttatgcc gctttatgtg catctaccat gatgatatca ctggtgaaag
     4741 agccatcatt ctgcaaggca aaatacgttc tcacctctgt cgatgcgctc tgttggtaaa
     4801 tgcgaatagc gtctactaac gcttctggcg tacgcattct cgcgacccat gaagaaaatt
     4861 ccagcggtaa cttatctgta attaaattat caactatcag attggcttca ttgattaacc
     4921 tcaaccactc accgctggcg tagtttcgta cgtgagaggt atcgcgtaat gcttctaccg
     4981 tctgtaacca gatgtcgcgc actgggtggc ccggagacat tacgtccatc acaatcagcc
     5041 tcccgccagg tttcaatatc cggttcactt cacgcagtgc tgcaccaaca tcatgccagt
     5101 gatgggcaga ataacggctg ataacaatat caaatgcgtt atcggcaaat ggcagacttt
     5161 cggcataccc ctggcgggtg gtgatatttt tcagttgccg gacttcggca gcttgtgcca
     5221 cgacatccag catttgggca gataagtcat acgccaccac cgtgctcacg ttttgcgcag
     5281 cgacaaagct ggcatgccct gctccgcagc ccatatcaag tacacttgca tcaggataat
     5341 cagccagaca caccgccagg cgctgtaaat ctcgtccgga tgcatgtacg gtactggtca
     5401 gatattcgct ggcttgagag ctaaactgct tttctacgtg gtcatggtgg gattgtgttg
     5461 tcactgcact gtccttttgt tgtttgagta taaaagggca gctccaaacg ttggtctgcc
     5521 ctgtggcaga cctgacatac cttatttatc aggcttgccg ggactgaatt caacgagtag
     5581 cggattgtga tcggacgcgc gcgtaaccag tacagaagct tcgctgacgt tcagaccacg
     5641 gtagaaaaca aaatcgagcg ggcgaccaaa cgcccgacgg cgctgatcat cggtaaaacg
     5701 cacctgacgc agcgacattt cccgcgcaaa acgatataac gcgttcatcc ttctacggct
     5761 ccaggcattg aaatctcccg ccataatgac cgggccgctg tggtgagcta tctgatcacc
     5821 aataggaagt aactgcttac tataaacatc cacgcccaga ctgaagttga cggcgtgtat
     5881 attaaccacc attaacaggc gggtgtcagg taatggatag accgtcacca gtgccgactt
     5941 cgccagacgc aaaatgggtt ctcgttcacg taacgggcag caatacactg gatgtgccgc
     6001 cgaaagggtc attacgccag aaggatgttg tggcagcacg aaagcgggta cctgatcggc
     6061 ggcaagatag ttagcagtcg caaactgtac caactctggc gttgtttgcg cttcctgcaa
     6121 taacaccaga tgtgcgtctt tgccgtaatt ctttaatacc gacaaccatt cagcgcgttg
     6181 ctgtttgtat atgttccaca ccaggatccg aatacgctct tcggtactta acggttctcc
     6241 gggtggtaat gcctggccga tgctcgcaaa agaccccggc ggtaagatcc tttccgcagg
     6301 ttgtccggca acatagcgca tggcataggt gttttttcgc acttttgact ttgaaacctc
     6361 tattacgtca acccttccgc actcgggagg gttctactgt tatagggatt ttagctcact
     6421 ctttcaacga gcaattgtat attgttatgt aagcaagtgc ttacaccggt cagaattttg
     6481 ctcattattg agaacattaa atcggatgac atatcacgca tcctgatggt ttaatgacaa
     6541 ataaattcaa aatatctctt ctattttgca acaggagcaa aaatgaaagc cacgtcggaa
     6601 gaactcgcca tttttgtttc ggtcgtagaa agcggcagct ttagccgggc agcggaacaa
     6661 ttagggcaag caaactctgc ggtaagccgg gcggtgaaaa agctggagat gaaacttggc
     6721 gttagcctgc ttaatcggac cacgcgacaa cttagcctga cggaagaagg cgagcgttat
     6781 tttcgtcgcg tacagtcaat tttgcaggag atggcagcgg cagaatcaga aattatggag
     6841 acgcgtaata caccgcgtgg actgttgcgc atcgatgccg caactccagt ggtgctgcac
     6901 tttctgatgc cgttaattaa gcctttccgt gaacgctatc cggaagtcac tttatcgcta
     6961 gtctcctccg aaacgattat taatttgatc gaaagaaaag tggatgtcgc gatacgcgct
     7021 ggtgcgttaa cggattccag cttacgtgcc aggccgttat ttaacagtta tcgaaaaatt
     7081 atcgcctccc ccgattatat ttcccgctac gggaagccag aaacgatcga cgatttaaag
     7141 caacatgttt gcctgggatt cactgaaccc gcttccctca atacctggcc gatagcctgt
     7201 agtgatggac aattacatga ggtgaagtac ggtttgtcat ccaatagtgg ggaaacactg
     7261 aaacagcttt gcctgagtgg caacgggatt gcgtgtttgt ccgactatat gatcgacaga
     7321 gaaatcgctc gcggagaatt ggtggagtta atggcagata aagtgttgcc agtggaaatg
     7381 ccattcagtg ccgtctatta cagcgaccgt gcggtaagta cgcgcatccg ggcttttatc
     7441 gatttcctta gcgagcatgt aaaaacagct cccggaggag ctgtcagaga ggcttaatcc
     7501 cattcaggag ccagaccttc cgggctaacc aggcggtcgt tgcaatccag tgcggcgatc
     7561 gcttttttat cttcggcatc aagctgtaaa ttttgtgcct taagattact ctccaggttt
     7621 tcacgtttag tagaagaagg aattactgag taaccttccc ccatagccca cgccagaatc
     7681 acttgtgccg gagtcgcatt gtgtttagct gcgatacgag caataacctc atctttcagg
     7741 gccttaccat acgccagcgt catataggaa gtaatatgga tgccgtgctg tttagcccag
     7801 gcaaccactt tacggttttg cagataagga gagagttcaa tctggttagt agcgatgttt
     7861 tcagcgccaa cagcagcaat cgccttttcc atcaatggga tcgtgaagtt ggaaataccg
     7921 atctcacgcg tcaacccttc ttttttggct tccagcagcg cctgcataaa ctcttcaaca
     7981 gagacttcat cgtttggtga cggccagtgg attagcgtca gatcaacata atcggtacgc
     8041 aatttttgca ggctctcttt cagacttggg atcaatttgt ctttgctgag attttcaatc
     8101 cagattttag tggtgatgta gagttcatga cgtggcacgc cactttctgc aatcgcctga
     8161 cctactgcgg cttcgttatc atagatttgt gcggtatcaa ttgcgcgata accaagttca
     8221 agcgccgttt tcacagatga aataacaacg tcgtctttca gacggaaagt acctaaacca
     8281 aatgcaggga tagccataat atgcctcttt taatgctatt ttcgttaact gaggtgatta
     8341 tgggcttcag actgttgagg aaaaagagcg taaaatgcag aggatttttg cgattctggc
     8401 aataatagat atacagacga aaaaaaacct cgagcgttaa ctcgaggctt cttaataagt
     8461 ggcggaacgg acgggactcg aacccgcgac cccctgcgtg acaggcaggt attctaaccg
     8521 actgaactac cgctccaccg aattctttta caaccaccgg ttttatgacc ggcttactgc
     8581 taaatttgat gcctggcagt tccctactct cgcatgggga gaccccacac taccatcggc
     8641 gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctacggcc
     8701 gccaggcaaa ttctgtttta tcagaccgct tctgcgttct gatttaatct gtatcaggcc
     8761 gaaaatcttc tctcatccgc caaaaca
//
LOCUS       DAZUCE010000205         8717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZUCE010000205 DAZUCE010000000
VERSION     DAZUCE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.205"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(105..725)
                     /gene="entD"
                     /locus_tag="ACHY03_004052"
     CDS             complement(105..725)
                     /gene="entD"
                     /locus_tag="ACHY03_004052"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFS3907227.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(900..3140)
                     /gene="fepA"
                     /locus_tag="ACHY03_004053"
     CDS             complement(900..3140)
                     /gene="fepA"
                     /locus_tag="ACHY03_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFS3907228.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3383..4585
                     /gene="fes"
                     /locus_tag="ACHY03_004054"
     CDS             3383..4585
                     /gene="fes"
                     /locus_tag="ACHY03_004054"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFS3907229.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4588..4806
                     /gene="ybdZ"
                     /locus_tag="ACHY03_004055"
     CDS             4588..4806
                     /gene="ybdZ"
                     /locus_tag="ACHY03_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFS3907230.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            4803..8684
                     /gene="entF"
                     /locus_tag="ACHY03_004056"
     CDS             4803..8684
                     /gene="entF"
                     /locus_tag="ACHY03_004056"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFS3907231.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQITNIYCAL
                     LRGEPTPASPFTPFADVVGEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFSDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLREHGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPHPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
BASE COUNT         1898 a         2335 c         2386 g         2098 t
ORIGIN      
        1 atccccgcat atccggttgt caggtcagag tggtaaggca cctgctttac actttcgccc
       61 gcagtcagtg atggctgcgg gcgaatcgta ccagatgttg tcaattaatc gtgttggcac
      121 agagttatga ctattttttc ttttatctgc cagtgcacag caaacatctc attctcacga
      181 tgaatgatga cctgctgttt attccagcta attatctgat agtccagaaa acctgcatca
      241 gtttggatct cacttgcctt aaatgcgctc tctttggcgg aaaatgccag tgtcagcgcc
      301 agagaaaagg ctaaaccgca gtctgcgagt cgctcgtgtt ctgctggtgt aataatgttg
      361 tctgtcaatt cggtcgctgt ttgcgcagaa aaaatttctt ctatatcaat gccaatcggt
      421 tgacgagata ccacggctaa tgccgtagtc ccacaatggc taatactgcc gtatacctcc
      481 gcaggccaca caggttggcg tagctcgccg attgcgggca cacatttata gccatattcc
      541 cgcaaggcat aaacagcagc gatccgtccg gctaaatgct ctgttttacg tttacgtcca
      601 gcgtgttgca gttgtgcgta gtgcggcagc cagagtaaat cctgctcaca aaaattcgcc
      661 ggatcgaact caacaaaatg cagcgtatgt ccggcaaagg ggagggaggt atgcgtagtt
      721 ttcatatcga ccatattcga gactgatgac aaacgcaaaa ctgcctgatg cgctacgctt
      781 atcaggccta catggtcctg caatatattg aattggcaag atttttgtag gccggataag
      841 gcgttcacgc cgcatccggc atgaacgacg cgcactttgt caacaatctg acgttagcat
      901 cagaagtgag tgtttacgct catataccac gtacgtcccg gctcgttata ggtatacgcc
      961 ccggcaccgg cgatatagtt ggcccctgcc aaatcgcccg tggtctgggc attacccgca
     1021 cgccacaaac gtttgtcgaa cagattgtcc acgccgccgg tcagactgac attcttcgtc
     1081 acatcccacg tcgcgctcag cccaacaatg ctgtaaggac taatttcttt ggtttccggt
     1141 ccaaccgctg gctgaccttt atagttgtac ttcttcggct gctgcttacc gtaccaggtg
     1201 aaggtcgttt gcatcgacaa atcttctcgt gcctgccagc tcagcgttga gttcaacgta
     1261 tactccggga tgatcgacaa acggtcgccc gtggttttgt tttcactctt cagcatataa
     1321 gtgatgttat tagtccacat caccgtttcg ctaaccggta cgtttaatga tccttccaga
     1381 ccttcaacca ccgctttcgg cacgttatcc cactgataga ggtcggtgcc gactgcgttt
     1441 tgccctacag caacatagcc tgcttcaatc ttattgcgat aatcgttacg gaaccaggtg
     1501 atgcccgcca gccagccgtc gcgtttgaac tccagaccaa tctctttgtt gatgctggtt
     1561 tctgctttca ggtcatcgtt accttgcaga tagcagccgc ccgcgctggc atagcagccc
     1621 tgacctttac tgtagagaat gtagttcggg ttagtctggt acaggctcgg cgctttatag
     1681 gcgcgggcaa tgcccatttt cagcgtgaag tcatcgccta aaccttgcga tatgttcagc
     1741 gccgggctcc agttattgcc gacaatactg tgatgatcga aacgcagccc cggcgttacg
     1801 atggtgctgt cagtcagctc catgttgttt tcggcaaaca gcgagaaaat ttctgctttt
     1861 gaatacgggc tacggtcggt ggcactcacg ccatcaatag cgccaccggt attcgttccg
     1921 gtcagtgcct gggtgttgga acttaagtcc ttcatccgtt gctgattcca ctccgtaccc
     1981 agcgtcagcg tctggttaac gaggaaatca atcggcaggt taacttcgct atgcagcatc
     2041 acgtcatcaa gatcgatatc gacgaaatcc tgtgtcgctt tttcgttaaa tttcccttcg
     2101 gtaccgcccg ccagaccttc cggaatacgc gagttacggg tgtgttcgta ctgcacccag
     2161 ttgctggtgg tcacgccgtt atcccagcca ccgttccagg tcagcgagta gttctggcga
     2221 tacaggcggt tggtttcatc gccatatttc gagcgagtgt aagcgtcaga gttggtgttc
     2281 tgggtatcgc ccgcatacag gttaccctgg cggctgtaac ccgcttccag ttccagcgat
     2341 tgcaatggtg cgaagtccca gcgcaccacg ccattaatat ctttattgat caccccttcg
     2401 cgcccggctg gtaacgtcgt ggcatacgtt ccggcacgcg cggactgatg accctggttg
     2461 atatcccacg cgtcagcctg ggttttgtcg aggttgccat acaagcggaa gctgaattcg
     2521 tcgcccagcg gaccggtcag gctaaagtta gtgcgtttgg tggcaccttc ctctttatgt
     2581 tctggcgcat tgaaatatgc gtcccaggag ccgtgccact cgccgctgcc ttttttggta
     2641 atgatgttaa ccacgccgcc cgccgcgccg ttgccataac gcgcagctgc cggaccacgc
     2701 agaacttcaa tacgttcaat catttcaggt ggcacccagg aggtatcacc acgggtatcg
     2761 cgctcgccac gccagccctg acgcaccgag ttacggctgc ttaccggctt gccgtcaatc
     2821 aaaatcagcg tgttttccgg acccataccg cgaatatcaa tctgtcggtt attccctcgc
     2881 tgaccactgg tggagttacc ggtcaggtta acgcctggca tggtacggat gatctccgaa
     2941 acatcgcggg caacggggtt tttgcggatt tcatctgcgg tgatggtcga aacgccaggc
     3001 gcctgtaagt tctgctcggc ggcggtaacg acaatagtat cgtcatgtga aacaggagta
     3061 tcggtcggct cttgtgcctg cgctacccca taaatcccca gattgaccaa caaggccagg
     3121 gaatgaatct tcttgttcat tgttttattc ctgcattttt gccacgaatt gcaactgtcg
     3181 ggcatggtcg tcatcaacac gacgcatccc gctaccgcga aaacctttga tcctgaaaga
     3241 cacgcagtgc agttggttaa ttaatgtccg cgcttcccac ggcgcgccat tacgctattg
     3301 caaatgcaaa tagttatcaa taatattatc aatatatttc tgcaatcaat gaaaaattgc
     3361 acagtaaaca tggggttatg gtgtgacggc gttaaaagta ggaagtgaga gctggtggca
     3421 gtcgaaacat ggcccggaat ggcagcgtct gaatgacgaa atgtttgagg tcactttctg
     3481 gtggcgtgat ccccaaggtt ctgaagaata ctcgacgata aagcgcgtat ggatctacat
     3541 cactggtgtg accgatcatc atcagaacag ccagccccag tcgatgcagc gaattgcagg
     3601 tactgatgtc tggcagtgga cgacacaact caatgccaac tggcgcggca gctactgctt
     3661 tattcccacc gaacgcgatg acattttttc tgcaccatcc cccgatcgcc tcgaattacg
     3721 tgaaggctgg cgaaaactat taccccaggc aatagccgat ccgctgaatc cacaaagctg
     3781 gaaaggcggg cgagggcacg ctgtttctgc actcgaaatg ccgcaagcgc ctctgcaacc
     3841 gggatgggat tgtccgcaag cgccagaaat accagccaaa gaaattatct ggaaaagtga
     3901 acggttgaaa aattcacgtc gtgtatggat ttttaccacc ggcgatgtaa cagcagaaga
     3961 acgcccgctg gcagttttgc tcgatggcga attttgggcg caaagtatgc ccgtctggcc
     4021 tgtgctgact tcgctgaccc atcgtcagca acttcctccc gccgtgtatg tgttgatcga
     4081 cgctatcgac accacgcacc gcgcccacga actgccgtgt aatgcggatt tctggctggc
     4141 agtacagcaa gagttattac ccctggtgaa agctattgcc ccttttagcg atcgcgccga
     4201 tcgcaccgtg gtcgccgggc agagttttgg tgggctgtcc gcgctgtatg ccggactgca
     4261 ctggcctgaa cgctttggct gtgtattaag ccagtcagga tcgtactggt ggccgcatcg
     4321 gggcgggcag caagagggcg tgttacttga aaagctaaaa gctggtgaag ttagcgccga
     4381 aggtctgcgc attgtgctgg aagcgggtat tcgcgagccg atgatcatgc gggccaatca
     4441 ggcgctgtat gcgcaattgc accctataaa agaatccatt ttctggcgtc aggttgacgg
     4501 cggacatgat gcgctttgtt ggcgcggtgg cttgatgcag gggctaatcg acctctggca
     4561 accacttttc catgacagga gttgaatatg gcattcagta atcccttcga tgatccacag
     4621 ggagtgtttt acattttgcg caatgcgcag gggcaattca gtctgtggcc gcaacaatgc
     4681 gccttaccgg caggctggga cattgtgtgt cagccgcagt cacaagagtc ctgccagcag
     4741 tggcttgaag cccactggcg tactctgaca ccggcgaatt tcacccagtt gcaggaggca
     4801 caatgagcca gcatttacct ttggtcgccg cacagcccgg catctggatg gcagaaaaac
     4861 tgtcggattt accctccgcc tggagcgtgg cgcattacgt tgagttaacc ggagaggtag
     4921 atgcgccatt actggcccgc gcggtggttg ccggattagc gcaagcagat acgttgcgga
     4981 tgcgttttac ggaagataac ggcgaagtct ggcaatgggt cgatgatgcg ctgatattcg
     5041 aactgccaga aattatcgac ctgcgaacca atattgatcc gcacggtact gcgcaggcat
     5101 taatgcaggc ggatttgcaa caagatttgc gcgtcgatag cggtaaacca ctggtctttc
     5161 accagctgat acaggtggca gataaccgct ggtactggta tcagcgttat caccatttgc
     5221 tggtcgatgg cttcagtttc ccggccatta cccgccagat caccaacatt tactgcgcat
     5281 tactgcgtgg cgaaccaacg cctgcttcgc cgtttacgcc tttcgctgat gtagtgggag
     5341 agtaccagca ataccgcgaa agcgaagcct ggcagcgtga tgcagcattc tgggcggaac
     5401 agcgtcgtca actgccaccg cccgcgtcac tttctccggc acctttaccg gggcgcagcg
     5461 cctcggcaga tattctgcgc ctgaaactgg aattttccga cggggaattc cgccagctgg
     5521 ctacgcaact ttcaggtgtg cagcgtaccg atttagccct tgcgctggca gccttgtggc
     5581 tggggcgatt gtgcaatcgc atggactacg ccgccggatt tatctttatg cgtcgactgg
     5641 gctcggcggc gctgacggct accggacccg tgctcaacgt tttaccgttg ggtattcaca
     5701 ttgcggcgca agaaacgctg ccggaactgg caacccgact ggcagcacaa ctgaaaaaaa
     5761 tgcgtcgcca tcaacgttac gatgccgaac aaattgtccg tgacagtggg cgagcggcag
     5821 gtgatgaacc gctgtttggt ccggtactca atatcaaggt atttgattac caactggata
     5881 ttcctggtgt tcaggcgcaa acccataccc tggcaaccgg tccggttaat gaccttgaac
     5941 tggccctgtt cccggatgaa cacggtgatt tgagtattga gatcctcgcc aataaacagc
     6001 gttacgatga gccaacgtta atccagcatg ctgaacgcct gaaaatgctg attgcccagt
     6061 tcgccgcgga tccggcgctg ttgtgcggcg atgtcgatat tatgctgcca ggtgagtatg
     6121 cgcagctggc gcagatcaac gccactcagg ttgagattcc agaaaccacg cttagcgcgc
     6181 tggtggcaga acaagcggca aaaacaccgg atgctccggc gctggcagat gcgcgttacc
     6241 agttcagcta tcgggaaatg cgcgagcagg tggtggcgct ggcgaatctg ctgcgtgagc
     6301 acggcgttaa accaggggac agcgtggcgg tggcattacc gcgctcggtc tttttgaccc
     6361 tggcgctaca tgcgattgtt gaagcaggtg cggcctggtt accgctggat accggttatc
     6421 cggacgatcg cctgaaaatg atgctggaag atgcgcgtcc gtcgctgtta atcaccaccg
     6481 acgatcaact gccgcgcttt gccgatgttc cagatttaac caacctttgc tataacgccc
     6541 cgcttacacc gcagggcagt gcgccgctgc aactttcaca accgcatcac acggcttata
     6601 tcatctttac ctctggctcc accggcaggc cgaaaggggt aatggtcggg cagacggcta
     6661 tcgtcaaccg cctgttgtgg atgcaaaatc attatccgct cacaggcgaa gatgtcgttg
     6721 cccaaaaaac gccgtgcagt tttgatgtct cggtgtggga gtttttctgg ccgtttattg
     6781 ccggggctaa actggtgatg gctgaaccgg aagcgcaccg cgacccgctc gctatgcagc
     6841 aattctttgc cgaatatggc gtaacgacca cgcactttgt gccgtcgatg ctggcggcat
     6901 ttgttgcctc gctgacgccg caaaccgctc gccagagttg cgcgacgttg aaacaggttt
     6961 tctgtagtgg tgaggcctta ccggctgatt tatgccgcga atggcaacag ttaacgggcg
     7021 cgccgttgca taatctatat ggcccgacgg aagcggcggt agatgtcagc tggtatccgg
     7081 cttttggcga ggaattggca caggtgcgcg gcagcagtgt gccgattggt tatccggtat
     7141 ggaatacggg tctgcgtatt cttgatgcga tgatgcatcc ggtgccgccg ggtgtggcgg
     7201 gtgatctcta tctcaccggc attcaactgg cgcagggata tcttggacgc cccgatctga
     7261 ccgccagccg ctttattgcc gatccttttg ccccaggtga acggatgtac cgtaccggag
     7321 acgttgcccg ctggctggat aacggcgcgg tggagtacct cgggcgcagt gacgatcagc
     7381 taaaaattcg cgggcagcgt attgaactgg gcgaaatcga tcgcgtgatg caggcgctgc
     7441 cggatgtcga acaagccgtt acccacgcct gtgtgattaa ccaggcggca gccaccggtg
     7501 gtgatgcgcg tcagttggtg ggctatctgg tgtcgcaatc gggcctgccg ttggatacca
     7561 gcgcattgca ggcgcagctt cgtgaaacat tgccaccaca tatggtaccg gtggttctgc
     7621 tgcaacttcc acagttacca cttagcgcca acggcaagct ggatcgcaaa gccttaccgt
     7681 tgcctgaact aaaggcacaa gcgccagggc gtgcgccgaa agcgggcagt gaaacgatta
     7741 tcgccgcggc attctcgtcg ttactgggtt gtgacgtgca ggatgccgat gctgatttct
     7801 tcgcgcttgg cggtcattcg ctactggcaa tgaaactggc agcgcagtta agtcggcagg
     7861 ttgcccgcca ggtgacgccg gggcaggtga tggtcgcgtc aaccgtcgcc aaactggcaa
     7921 cgattattga tggtgaagag gacagctccc ggcgcatggg attcgaaacc attctgccgt
     7981 tgcgtgaagg taatggcccg acgctgtttt gtttccatcc ggcatccggt tttgcctggc
     8041 agttcagcgt gctctcgcgt tatctcgatc cacaatggtc gattatcggc attcagtcgc
     8101 cgcaccccaa tggccccatg cataccgcgg caaatctgga tgaagtctgt gaagcgcatc
     8161 tggcaacgtt acttgaacaa caaccgcacg gcccttatta cctgctgggt tattcacttg
     8221 gcggtacgct ggcgcagggt attgcggcgc ggctgcgtgc ccgtggcgaa caggtggcat
     8281 ttcttggctt gctggatacc tggccgccag aaacgcaaaa ctggcaggaa aaagaagcta
     8341 atggtctgga cccggaagtg ctggcggaga ttaaccgcga gcgcgaggcc ttcctggcgg
     8401 cacagcaggg aagtacttca acggagttgt ttaccaccat tgaaggcaac tacgctgatg
     8461 ctgtgcgcct gctgacgact gctcatagcg taccgtttga cggtaaagcg acgttgtttg
     8521 ttgctgaacg cacgcttcag gaaggtatga gtcctgaacg cgcctggtcg ccgtggattg
     8581 cggagctgga tatctatcgt caggattgtg cgcatgtgga tattatctct ccaggggcat
     8641 ttgaaaaaat tgggccgatt attcgcgcaa cgctaaacag ataaattaac attatctata
     8701 aacccataat tgcagga
//
LOCUS       DAZUCE010000206         8678 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZUCE010000206 DAZUCE010000000
VERSION     DAZUCE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.206"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            122..556
                     /gene="slyA"
                     /locus_tag="ACHY03_004057"
     CDS             122..556
                     /gene="slyA"
                     /locus_tag="ACHY03_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFS3907232.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(602..1069)
                     /gene="slyB"
                     /locus_tag="ACHY03_004058"
     CDS             complement(602..1069)
                     /gene="slyB"
                     /locus_tag="ACHY03_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFS3907233.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1343..2452
                     /gene="anmK"
                     /locus_tag="ACHY03_004059"
     CDS             1343..2452
                     /gene="anmK"
                     /locus_tag="ACHY03_004059"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFS3907234.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            2550..2879
                     /gene="mliC"
                     /locus_tag="ACHY03_004060"
     CDS             2550..2879
                     /gene="mliC"
                     /locus_tag="ACHY03_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFS3907235.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNSQR"
     gene            2938..3594
                     /gene="pdxH"
                     /locus_tag="ACHY03_004061"
     CDS             2938..3594
                     /gene="pdxH"
                     /locus_tag="ACHY03_004061"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFS3907236.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3723..4997
                     /gene="tyrS"
                     /locus_tag="ACHY03_004062"
     CDS             3723..4997
                     /gene="tyrS"
                     /locus_tag="ACHY03_004062"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFS3907237.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            5056..5919
                     /gene="pdxY"
                     /locus_tag="ACHY03_004063"
     CDS             5056..5919
                     /gene="pdxY"
                     /locus_tag="ACHY03_004063"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFS3907238.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5963..6568)
                     /gene="gstA"
                     /locus_tag="ACHY03_004064"
     CDS             complement(5963..6568)
                     /gene="gstA"
                     /locus_tag="ACHY03_004064"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFS3907239.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAESLK"
     gene            complement(6674..8176)
                     /gene="dtpA"
                     /locus_tag="ACHY03_004065"
     CDS             complement(6674..8176)
                     /gene="dtpA"
                     /locus_tag="ACHY03_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFS3907240.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         2264 a         2125 c         2186 g         2103 t
ORIGIN      
        1 tctgaaaaat atgtctgacg gtaaccaaat gcagcaatac atttgtttta gcaatacaat
       61 tgctgcacac tattctaaaa gccgcataat atcttagcaa gctaattata aggagatgaa
      121 attggaatcg ccactaggtt ctgatctggc acggttggtg cgcatatggc gtgctctgat
      181 agaccatcgc ctgaaaccgc tggagttaac acaaacccat tgggttacgt tacacaatat
      241 ccatcagtta cctccagacc agtcgcaaat tcaactggca aaagcgattg gcatcgagca
      301 gccatcactg gtccgtactc tggaccaact ggaagaaaaa gggttaattt cgcgtcaaac
      361 ttgtgccagc gatcgtcggg ctaaacgtat taaactgacg gaaaaggcag agccgctgat
      421 cagcgaaatg gaagctgtta ttaacaaaac ccgcgcggaa attttacatg gcatctccgc
      481 agaggaactg gaacaactga ttacgctcat cgcaaaactt gagcataata tcattgagtt
      541 acaggccaaa gggtgaaatg aagggggcgt gtggtcagta acctgaccac acgcaaacta
      601 tttagcgcgg agaaacggtc acctgactgc cattgctggc cagtacgaca cgttggcccg
      661 gagagaaacg agtgttgcct tgtttctgta ccaccatgat ggtattacca tcgtctttac
      721 gaatttccag ctcgacacct tgcgttttgt tcattgcact ctgtacgccc tgaccagcta
      781 cgccacctgc aacagcgcct gctgcagtag ccagagaacg cccggttccg ccaccaacag
      841 tatttcccag gaaaccacca agaacagcac cgccaattgc accgatcacg ttggaatcat
      901 caccgccctg aatctgtacc ggacgtacgt taacgatggt tccatagctg acattctgta
      961 cttgtttcgc ttcagaagcg gtataaacat cccctgacag ggtgtcgtta ttaacacaac
     1021 cgacaagaga cagacctacc attgaaacaa ccaatacgcg tttaatcatt gaaaaatctc
     1081 ctgttcacca tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg gcatatgtga
     1141 atccatggtt acatgttcta ccaaatatga gcaaatcata atgaaagtag gatcaaccaa
     1201 ttataaacaa aacattcatg atgattattg atttgaagat aatgttttac taccaacggt
     1261 aaaaatcggt cattccgcat ggtattcacg aaaagtttat gtttgagtgg tgcgcaaacg
     1321 tcagattgaa aaaggatgaa ctatgaaatc gggccgcttt attggcgtta tgtcaggcac
     1381 cagccttgat ggtgttgatg ttgtgttggc gacaattgat gaacaccggg tcgcacagct
     1441 ggcaagtttg agctggccga tcccggtgtc tctgaaacag gctgtactgg atatttgcca
     1501 gggccagcag cttacacttt cgcagtttgg acagcttgat actcaactcg ggcgactttt
     1561 tgctgatgcg gtcaatgcct tgcttaagga acaaaacctg caggcgagag atatagttgc
     1621 gatcggctgt cacggtcaaa ccgtctggca tgaaccgacg ggcgtggcac cacacacttt
     1681 acagattggc gataacaatc aaattgtggc acgcaccgga attacggttg tcggtgattt
     1741 tcgccgtcgc gatattgcct tgggaggaca aggcgcaccg ctggtacctg cgttccatca
     1801 tgccttgctg gctcacccaa ccgagcgacg aatggtgctc aatattggcg gcatcgccaa
     1861 tctgtcactg ctcattcctg ggcagccggt tggcggctac gataccggtc ctggtaacat
     1921 gctgatggat gcctggatct ggcgtcaggc cggtaaacct tacgataaag atgccgagtg
     1981 ggcacgggcg ggtaaagtta ttctcccact gctgcaaaat atgctcagcg acccatattt
     2041 ctcgcaacct gcaccgaaaa gcaccggacg cgaatacttt aactacggct ggctggagcg
     2101 ccatttacgc cattttccgg gtgttgatcc ccgtgatgtg caggccacac tggcagaact
     2161 caccgccgtg accatttctg aacaagtttt gttgagcggt ggctgcgaac gattgatggt
     2221 atgtggtgga ggtagtcgta atccgctact catggcgcgt ctggcggcat tactgccagg
     2281 cacagaagtc accaccaccg atgccgttgg cattagtggc gatgacatgg aagcattggc
     2341 tttcgcctgg cttgcctggc ggacgctggc gggattacca ggaaatctgc cttccgtcac
     2401 tggcgcaagc caggagacgg tactgggggc tattttcccc gctaacccgt gatatcagag
     2461 ttaactgaat ttttcttacc gcgtctgccg ttacacttgg aacgttaggg agggcgtatt
     2521 gccctccaga ccaggaaagt cttcgggaaa tgtctatgaa aaaactgtta ctgatctgtt
     2581 tgccggtgct tctcaccggc tgtagcgcgt tcaatcaact ggttgagcgc atgcaaaccg
     2641 atacgctgga ataccagtgt gatgaaaaac cgttgacggt aaaactgaat aatccgcgcc
     2701 aggaagtcag ttttgtttac gataatcaac tactgcatct caaacagggc atttcagcct
     2761 ctggtgcacg ttacactgac ggaatctatg ttttctggtc gaaaggcgag gaagcgactg
     2821 tctataaacg cgaccgcatc gtcttgaata actgtcagtt acaaaattca cagcgttgag
     2881 atttttccag gggcggcgca caatagcgtc acccactgac aatccgtaaa gaaaaccatg
     2941 tctgataacg acgaattgca gcaaatcgcg catctgcgcc gtgaatacac caaaggcggg
     3001 ttacgccgcc gcgatcttcc cgccgatcca ttaacccttt ttgaacgttg gctctctcag
     3061 gcttgtgaag ccaaactggc ggaccctacc gcgatggtgg tcgctaccgt ggatgaacat
     3121 ggtcagcctt atcagcgcat cgttttactc aaacattacg acgaaaaagg catggtgttt
     3181 tacaccaacc tcggtagccg taaagcacat caaattgaaa ataatccgcg cgttagcctg
     3241 ctgttcccgt ggcataccct tgaacgccag gtgatggtga tcggtaaagc agaacgactt
     3301 tcgactctcg aagtgatgaa atattttcat agccgcccgc gtgatagcca gattggtgca
     3361 tgggtttcga agcagtccag tcgcatttct gcccgcggta tccttgaaag taaattcctg
     3421 gagctgaagc agaagtttca acagggcgaa gtgccattgc cgagcttttg gggcggtttt
     3481 cgcgtcagcc ttgaacagat tgagttctgg cagggtggtg agcatcgcct gcatgaccgc
     3541 tttttgtacc agcgtgaaaa tgatgcgtgg aagattgatc gtcttgcacc ctgaaaagat
     3601 gcaaaaatct tgctttaatc gctggtactc ctgattctgg cactttattc tatgtctctt
     3661 tcgcatctgg cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac atggagattt
     3721 tgatggcaag cagtaacttg attaaacaat tgcaagagcg ggggctggta gcccaggtga
     3781 cggacgagga agcgttagca gagcgactgg cgcaaggccc gatcgcgctc tattgcggct
     3841 tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt tccattgtta tgcctgaaac
     3901 gcttccagca ggcgggccat aagccggttg cgctggtagg cggcgcgacg ggtctaattg
     3961 gcgacccgag cttcaaagct gccgagcgta agctgaacac tgaagaaact gttcaggagt
     4021 gggtggacaa aatccgtaag caggttgccc cgttcctcga tttcgactgt ggagaaaact
     4081 ctgctatcgc ggcgaacaac tatgactggt tcggcaatat gaatgtgctg accttcctgc
     4141 gcgatattgg caaacacttc tccgttaacc agatgatcaa caaagaagcg gttaagcagc
     4201 gtctcaaccg tgaagatcag gggatttcgt tcactgagtt ttcctacaac ctgttgcagg
     4261 gttatgactt cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt ggtggttctg
     4321 accagtgggg taacatcact tctggtatcg acctgacccg tcgtcttcat cagaatcagg
     4381 tgtttggcct gaccgttccg ctgatcacca aagcagacgg caccaaattt ggtaaaactg
     4441 aaggcggcgc agtctggttg gatccgaaga aaaccagccc gtacaaattc taccagttct
     4501 ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc tttatgagca
     4561 ttgaagagat caacgccctg gaagaagaag ataaaaacag cggtaaagca ccgcgcgccc
     4621 agtatgtact ggcggagcag gtgacccgtc tggttcacgg tgaagaaggt ttgcaggcgg
     4681 caaaacgtat taccgaatgc ctgttcagcg gttccttgag tgcgctgagt gaagcggact
     4741 tcgaacagct ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc gcagacctga
     4801 tgcaggcgct ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt aaaactatcg
     4861 cctccaatgc catcaccatt aacggtgaaa aacagtccga tcctgaatac ttctttaaag
     4921 aagaagatcg tctgtttggt cgttttacct tactgcgtcg cggcaaaaag aattactgtc
     4981 tgatttgctg gaaataatgc attaagtgga aagggggagt gagaattcac tccccctggt
     5041 ttttatacag ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt tgtttatggt
     5101 catgcgggta acagtgcggc agagtttccg atgcgccgcc tgggcgcgaa cgtctggccg
     5161 ctgaacaccg ttcaattttc taatcacacc caatacggca aatggactgg ctgcgtgatg
     5221 ccgcccagcc atttaaccga aattgtgcaa ggcattgccg ccattgataa attacacacc
     5281 tgtgatgccg tattaagtgg ctatctggga tcggcggagc agggtgaaca tatcctcggt
     5341 atcgtccgtc aggtgaaagc cgcgaatccg caggcgaaat atttttgcga tccggtaatg
     5401 ggtcatccgg aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca tgtgcggcac
     5461 ggtttgcctg ccagcgatat cattgcgccg aatcttgttg agctggaaat actctgtgag
     5521 catgcggtaa ataacgtcga agaagcggtt ctggcagcgc gcgaactcat tgcgcaaggg
     5581 ccacaaattg tgttggttaa acacctggcg cgagctggct acagccgtga ccgttttgaa
     5641 atgctgctgg tcaccgccga tgaagcctgg catatcagcc gtccactggt ggattttggt
     5701 atgcgccagc cggtaggtgt tggtgatgtg acgagcggtt tactgctggt gaaactgctt
     5761 cagggggcaa cgctgcagga ggccctggaa catgtgaccg ctgcagtcta cgaaatcatg
     5821 gtgaccacca aagcaatgca ggaatatgag ctgcaagtgg tggctgctca ggatcgtatt
     5881 gccaaaccag aacattactt cagcgcaaca aagctctgaa atatctcatt tcggcccaca
     5941 acgacctgtg ggccgatggc tattacttta agctttccgc tgacagcgcg tcttgtactt
     6001 ccggacgttc agccatacgt tgcataaatg ccgcaatgtg ctctaaccct tccagattca
     6061 gtttcaccgc gtatgcccag cgcagaaccg taaacagata ggcatcagca attgtaaatc
     6121 tttgcccgca gatccagtgc tcatccttca gtgcctcgtt cacatattgc agcttcttct
     6181 ccagttgcgc gcgaactgtc ggtttgtact cttccggtgt atcagggcga aacagaggtg
     6241 tgaaaccttt atgcagctcg gtggcgatgt aattcagcca ttcgatggtt ttatagcggg
     6301 aaatactgtt taccggtgcc agcaactggc ggtcggggac gctgtcggca agatactgca
     6361 taatcgctac accttccgtc agcaaagtac cgtcatccag cagcaatgca ggtacctgtc
     6421 ccttagggtt aacggcaaag taatcgtcac cgttttcgag acgttttttc attaaatcca
     6481 cactgacgag cgtaaaatcc tttccgctct cacgcagggt gatatgggaa gcgagagagc
     6541 aggcgcccgg tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg tttaaagtat
     6601 ggtagtgcgt tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc ttgtaaaaga
     6661 gtttccctga attttacgct acagcagctt tcgccgcttt gtcagcagcg tcatcctgcg
     6721 tcatgcggtg cagtttcggc gcggtaaaca gcatcagtac tgcaataacg gcagtagcaa
     6781 caccaatctg caagaatacg cgaccataga cttccagtga catcagcgga tcggtaacgt
     6841 tatccggcac agccatcata cccgcaacat aaccaccaat caggtttgca ccggcagtgg
     6901 tcaggaacca gctacccata atgaagccca tcagacgctg cggaacgagt tgtgcaacca
     6961 ttgccagacc cagaccagag atcatcagtt ccccgatact ctgcaggcca tagctcgcga
     7021 ccagccagct tacagacacg atacctgcgt cagacgcgaa tttcgcaccc agcggcagaa
     7081 tcaggaacgc accagaacac atcaccatac cgattgcaaa cttggttggc atcggcaggg
     7141 tatcgcccat cttgttatag atagcggcca gaatcgggct accaatgatg atccagaacg
     7201 ggttcagtgc ctgatactgc tcaggttcta cggccaggcc cagaatggtg tgttcaacgt
     7261 tacgaatcgc aaagaagttc agtgacgttg gcatctggct gtacagcacg aagaagataa
     7321 tggcttcgag catcaggatg aaggcaacga tcattttacg acgcgcagca cctttcatcg
     7381 cgaaggcttc tttaccgaag atcaccacga taccgaaggc aacaacgccc agcgccatac
     7441 gcgcaacttc ctgattgtgc agcagccagg tggcgatagc gatcagtgcc acaacaccaa
     7501 taatggtcag cagcaggtta cggtagttga taggctcgaa gtctggtttt gaaccgtact
     7561 gtttaaccca gcgttggcag aaggcgaagt taacgatagt gatcagcagg cctacaacgc
     7621 tcaacgcaaa cgcaacactc cagccgtatt tcgcggccag ccacggggtg gcgatcatag
     7681 agaagaaaga gccgatgttg acggacatgt agtacatggt gaatgcaccg tccagacgtg
     7741 ggtcgttttt ctcatagcat gtagaaagca gagaagacgg gttagcctta aacaggccgt
     7801 taccgaccgc aatagccgcc atacccatat aaacgatacc ggcgtcgtga ccagaccagg
     7861 caaccagagc ataaccaatc gccagcacaa tagcgccaag cataattacg cgtttagtac
     7921 ccagtacctt gtcacctaac cagccgccga tagcgaccag accataaacc agggcactaa
     7981 aggaagagaa aagggtgatt gaatccgctt cagacatacc cagttgttta accaggtaaa
     8041 cagccataat tccttgtagg ccgtaataac caaaacgttc ccataactca atcgagaaga
     8101 tgagatagaa cgccttcggt tgtttgaaag cgttcaaact gacgctttca gttggttttt
     8161 ggtttgcagt ggacactttt acctcttttt tacatcccat attaacgggg gtgctttctc
     8221 gcgttatgtc taaaaaacat acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat
     8281 gttcactatc ttgcactttc agacaagtcc tttgtaataa tctgttacat ataactgagg
     8341 cggggaattc aaccattccg ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac
     8401 ggcagatttt ttcactgcat tgagaggggg atgatggctt gacggcgata tgttctgcta
     8461 tttagcgttt ttcctagtga gatagtccag tttctgaaaa atagccagtg taatgttttg
     8521 taggtcaata tttgttgcgt gtgtaaattt aaatgatttt aacctgattc ttgcaatgtt
     8581 tttacattgc cacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa
     8641 aaaacaatta acctgcattt ttggttatcc gcaaacgg
//
LOCUS       DAZUCE010000207         8580 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZUCE010000207 DAZUCE010000000
VERSION     DAZUCE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.207"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..211)
                     /locus_tag="ACHY03_004066"
     CDS             complement(<1..211)
                     /locus_tag="ACHY03_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907241.1"
                     /translation="MCNNIRPDAAAEAIITLMHALIDISVIADMAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIA"
     gene            complement(201..434)
                     /locus_tag="ACHY03_004067"
     CDS             complement(201..434)
                     /locus_tag="ACHY03_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907242.1"
                     /translation="MSQEITLQQAAERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLSDEQERRTLISNEKKCGGVHHVQ"
     gene            complement(431..904)
                     /locus_tag="ACHY03_004068"
     CDS             complement(431..904)
                     /locus_tag="ACHY03_004068"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFS3907243.1"
                     /translation="MAFTISCGTRAFRACDFAFVVPVAISIPLLDWWGTVYTEKAQIK
                     PLTWATPKAYSGPHLEIIKVHKCKAPQVSLPLAGLLTTNDSESIEVAMLNHTTHPQGR
                     DSHNLNKYIWRFIALSTAQPRVITIEATSEQEARQQSPAGCVMVFAARIRQGVGL"
     gene            831..1010
                     /locus_tag="ACHY03_004069"
     CDS             831..1010
                     /locus_tag="ACHY03_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFS3907244.1"
                     /translation="MATGTTNAKSQALKARVPHEIVNAMESVKESGESTSQFIIASMQ
                     GEIKRRQRRKAKEQE"
     gene            complement(1770..1958)
                     /locus_tag="ACHY03_004070"
     CDS             complement(1770..1958)
                     /locus_tag="ACHY03_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3907245.1"
                     /translation="MLSTDRFIREKECQQLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCEAVH"
     gene            complement(2324..3553)
                     /locus_tag="ACHY03_004071"
     CDS             complement(2324..3553)
                     /locus_tag="ACHY03_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3907246.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGARPERIKLGNYPDLSLKAAREKAAQCRAWLAEGKNPRHEMNYTVQK
                     ALKPVTVGDALTYWLESYVKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLKHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRDALDMWTERLGI
                     LAGTHENVTTLPIAREI"
     gene            3802..4923
                     /gene="roxA"
                     /locus_tag="ACHY03_004072"
                     /gene_synonym="ycfD"
     CDS             3802..4923
                     /gene="roxA"
                     /locus_tag="ACHY03_004072"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFS3907247.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDMLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(5069..6298)
                     /gene="pepT"
                     /locus_tag="ACHY03_004073"
     CDS             complement(5069..6298)
                     /gene="pepT"
                     /locus_tag="ACHY03_004073"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFS3907248.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            6548..7684
                     /gene="potA"
                     /locus_tag="ACHY03_004074"
     CDS             6548..7684
                     /gene="potA"
                     /locus_tag="ACHY03_004074"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFS3907249.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            7668..8531
                     /gene="potB"
                     /locus_tag="ACHY03_004075"
     CDS             7668..8531
                     /gene="potB"
                     /locus_tag="ACHY03_004075"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFS3907250.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDD"
BASE COUNT         2055 a         2161 c         2089 g         2275 t
ORIGIN      
        1 cagcaatcag gatggctttg gcctcattca gcgccatatc cgcactaagt tgcataaccg
       61 ccagcgaatg aggaacgaaa gccccggcat attctgtttc actggtggcg tgcttatgcg
      121 ccatgtctgc gataacagaa atatcaatca gcgcgtgcat tagcgttatg atggcttcgg
      181 cggctgcgtc cggacggatg ttattgcaca tggtgtaccc ctccgcattt tttttcgtta
      241 gaaataagcg ttcttctttc ctgttcatcg ctcaggaata cgcagacctc accgctaagg
      301 cgtttaagta agccgatgat tgcccctgat tcgctgtcgg tcatcatgcc agggtaatcc
      361 tctgccagtg cgcaaataac ttcgatttgg tgggcgcgtt ctgctgcctg ttgtagtgtg
      421 atttcctggc tcataagcct accccctggc gaatacgggc agcaaatacc atcacgcagc
      481 cagccggaga ttgctggcgt gcttcctgtt cgctggtggc ctcaatggta atcacgcgcg
      541 gttgtgccgt gctcagggcg ataaaacgcc agatgtattt attcaggttg tgcgagtccc
      601 gcccttgcgg gtgtgtggta tgatttaaca tagctacctc gatactttcg ctatcgttgg
      661 tggttagaag ccctgcgagt ggtaacgaca cttgcggggc tttgcattta tgcaccttga
      721 taatctcaag gtgtggccca ctatatgctt taggtgtggc ccacgtcaag ggttttattt
      781 gtgctttttc tgtgtatact gtcccccacc aatccaacag aggaatagaa atggcaacgg
      841 gtacaacaaa cgcaaaatca caagctctaa aggctcgtgt accacacgaa atagtaaacg
      901 ccatggaatc agtgaaagaa tcaggcgaaa gcacatcaca attcatcatt gcgtcaatgc
      961 aaggcgagat caaacgccgc cagcgccgca aggccaaaga gcaggagtaa ccatcaccag
     1021 cgccgtggtg tgagtaacta cggcgcattg ctatgcagga caacacaatg accgataaag
     1081 aattgaccaa aacattatca ccggcacgga aaagacggcg cagaaagata gagcatgaat
     1141 cagaaagatt cgcgccatgt gcttttgccc ttgagcaatt ccttaaagag tacagggaaa
     1201 agcgctcatt gcaggtatgg caacgaactg aacctgactg attgcattgc ccaccagccg
     1261 caaatgtggc attgttggca atgctcatgc gttggggata acgtgtagct tgtgtcgagg
     1321 ggccaccgta gcgggtggcc tttgttttgc ccgttattcg gcaattgtgg cgcttctcca
     1381 catggttgat ataatcccac tgctcagatt cattttttgc gcagtaggtt aattgttcac
     1441 aaaggcgctc cggcaacggg gcgctttttg tttttattag tttgtagaaa cctgactcag
     1501 agataatgct catattctga ttgccgtcag gggtgtaagt taaatttact ccctttttat
     1561 catcaaacat ctgcaacgct ctggcgttaa caatattggt ttcactcccg ccagtaaggc
     1621 cggaaataac cggaataatt tcggcaaaat tttgcagatt ctgtttttca ggacgaacga
     1681 agccccgccc ttgttcggga gaatatccag tattcatggt tagatctctg tattagtgga
     1741 tgggtggcgg ctgtgtgccg ccagcctgat tagtgaactg cctcgcagct gtccttccat
     1801 gccagaactt cggataaaga ccagccaacg gaacgaccgc ccagcttacg ccgtgatggg
     1861 aattgtccgg ctttttccag gcggtaccga catgagcggc tcaggcctgt aagttgctga
     1921 cattcttttt cacgtataaa gcgatctgtg cttaacacaa ttcccccttt gttgtttctt
     1981 aaagagtcat tgggtggatt attgggttgg gttgtgtcgg attgtgtcag ggtgtttgtg
     2041 gaatggcaaa cactggcggc ggtcgtttta cagaagcagg ggaaatgaga ataaaaactc
     2101 ttttaattca ttatgatgca aaggcataaa attttacttt tacgcctttt ttacatattt
     2161 ttaagagtga ttcgccagtg tataaaaaaa cagtacacct ataaaaatca aatggttatt
     2221 acgatggctg atttttaacc cgttgttcca ttttgttcct tgttgttcgt cgttgtgtct
     2281 cgttgtttca cgttgtccgc atcctgaaaa atcgcaaaaa aaattatatt tctctggcta
     2341 ttggcaatgt ggttacgttt tcatgtgttc ccgccagtat ccctaaccgc tccgtccaca
     2401 tatccagcgc gtcacgtttc gcatccagat aacgggaatg gttatagact cgttgcatcc
     2461 ctggcatctg atgacctgta agctgctcca cgacgtgagg atccacgcct aaatcgttca
     2521 gcatcgttgt aaaggtgcgc cggatgtcat gcagtgacca gtgagggtgt tttagcctcc
     2581 tgtgcgctaa tctgccgtac tgcgacacgc ttgtttcctg tttcacttcc cccagcaata
     2641 agcccgtgtg cctgttctgc tccaccagct gcgtgacgaa cggcagtatt gcttccggta
     2701 tgggccggaa tattgccacc ttcgttttgc tgtgttcttt cggcacggtc cagagcattt
     2761 cggtaaaatc ccactcgctg atctccgata acctcagttc taccgtccgg catccgaaaa
     2821 caatcaggag gcggattagt gcgacgtagt aaggggaaaa tatttttttg tccagtgcct
     2881 gcaataattc gccaagttct ttggtgctta agacacgctc gcttatatcc ggttttttcc
     2941 caacgtccgc cacactcata tcatcaagaa cgttgctgat tgcatagcgc cgcctccggc
     3001 agaacttaag cgcctgtttg cacgtctgta gcaagaatcc ggcagtaaca ggcgttcgct
     3061 ttgccacctg gtcaaaacag gccagccagt gccgtagctc gcatttatcc agcggcatag
     3121 caccaatgtg ctgtattacg tgattattaa ggcgcttttt cagggcggca taatccacgc
     3181 ggttttcctt tacgtaagac tcaagccagt aggtaagcgc atcgccaacc gttacgggct
     3241 ttaacgcttt ctgtacggtg taattcatct catgacgtgg atttttcccc tcagcaagcc
     3301 aagcgcgaca ctgtgccgct ttttccctgg cagctttcag actcagatca ggataatttc
     3361 ccagcttaat ccgttccggt cgtgctcccc ttcccgttcc ggccctgtag gtgaaatacc
     3421 aggttaaaag gccgctggtt gaatgcctga cgctcaggtt tccaccgtca ttaagaaagg
     3481 ctgttttttg ggcgggtgtg ccgttgattt tcctcagctg tgtatcgctc agtttgttaa
     3541 gtgctctgct cataattttg attccggtca tcacaattaa ctacaccatt aactacaccg
     3601 atcgttgcac aaagggctac aacgtgaaac aagctggaac aaggaaaatc ataaatctgt
     3661 ttataatcaa aaacatagtg aacaatctga aacattatga aacggcaaaa aacactaatt
     3721 gataaaatat gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat
     3781 accagcctgt ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt
     3841 gaacgtcact ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac
     3901 ccgatctctc cagacgagtt ggcgggtctg gcgatggaaa gcgaagtcga cagtcgactg
     3961 gtcagtcacc aggatggcaa atggcaggtc agccacggtc cgttcgaaag ctacgatcat
     4021 ctcggtgaaa ctaactggtc actgttagtg caagcagtga accactggca tgagccgacc
     4081 gccgcgctga tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatc
     4141 tctttttctg tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc
     4201 attcagggta ccggacgtcg tcgctggcga gtgggcgaaa agttgcaaat gaaacagcac
     4261 tgcccacatc cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg
     4321 gagcctggcg atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa
     4381 aatgcgatga actattccgt gggttttcgc gcgccaaata cgcgggaatt aattagcgga
     4441 tttgccgatt atgtgctgca acgtgaactg ggcggcaact actacagcga tccggatgtt
     4501 ccacctcgcg ctcatcctgc ggacgttctg ccgcaagaga tggataaact gcgtgagatg
     4561 atgctcgaat tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc
     4621 cagtcacgtc atgaactgga tatcgcgccg ccagagccgc cttatcagcc agatgaaatc
     4681 tacgatgcgc tgaaacaagg tgatatgctg gtgcgcctgg gtggtctgcg cgtattgcgc
     4741 attggcgacg acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg
     4801 gatgcactcg ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat
     4861 ccgtcattcc tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg
     4921 taagtttgga tttaagccgg atgtggcgct gattgcgcct tatcgtgcct gcatgcactg
     4981 aacgcctgta ggcacgataa gtatgtggac cagtgtcaat ataacggttt catcccggta
     5041 attggcagtc cacatacgca tcgggcaatc acgacttccg ttgcgccgtt aactcggcaa
     5101 tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac tcatgcttac
     5161 catgatagtt gtaaccgcca gtgaacaggt tcgggcacgg taatcccata aacgacaact
     5221 gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg cagtcacgca
     5281 tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctcg cgcatattgt
     5341 agtaactgtc ttcaatcacc aattcaatgt agcaatcagg atgtaaccct ttgcccactt
     5401 ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg tcgaaatcac
     5461 ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc aggtgataga
     5521 aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc gcatgaatac
     5581 gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga tgaacattgt
     5641 taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc agttcgccta
     5701 cgccaccacc atcaacagtg taagcccagc gggcatcgaa ggcgtcaaca tcaaaatgtt
     5761 tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca ccatgcggaa
     5821 tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaataccg gctttgtcat
     5881 cggcacctaa caaggtttta ccatcggtag taatcagcgt ctgacccagt agctgatgca
     5941 gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca atatcgccac
     6001 cgcgatagtt ttcaacaatt tgcggattca catttttgcc gctgcaatcc ggtgaggtat
     6061 ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc ggtaacgtcg
     6121 ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatctct tcgagctgct
     6181 ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt ctcacccctg
     6241 cttttgattg ggtatccaga gacacgtagt tcaaaaatcg ctcaagtagt ttatccatgt
     6301 agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa atattgcgtc
     6361 aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat caatataaag
     6421 ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta gaattaccgc
     6481 cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag ccatccgttg
     6541 cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt caccgctggt
     6601 gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc agctggatct
     6661 gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg gtaaaacaac
     6721 cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca tgttggataa
     6781 cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt tccaaagcta
     6841 cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc gcatgcaaaa
     6901 aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg tgcagctgga
     6961 aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac gcgtcgccat
     7021 tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc tctcagcgct
     7081 ggattacaaa ctgcgcaagc aaatgcagaa cgagctgaaa gcgttacagc gtaagcttgg
     7141 cattacattc gtcttcgtga ctcacgacca ggaagaagca ctcaccatgt cagacaggat
     7201 tgtggtgatg cgcgatggtc gcattgagca agacggcacg ccgcgtgaaa tctacgaaga
     7261 gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta acgccactgt
     7321 catcgaacgt ctagacgagc agcgcgtacg cgccaacgtt gaaggccgcg aatgtaatat
     7381 ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc gcccggaaga
     7441 cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg gttacgttcg
     7501 cgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa atggcaagat
     7561 ggtgatggtc agcgaattct tcaatgaaga cgatcctgac tttgaccact ctctcgacca
     7621 aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg aagaacacaa
     7681 gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg tttgtctttc
     7741 tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc agttttgtca
     7801 aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat tttgaagtgc
     7861 tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg ctgggctacc
     7921 cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg ctgtttctgc
     7981 tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa attttcctca
     8041 gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac acaccaatcc
     8101 gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg ctaccgttta
     8161 tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt gaggcggcgc
     8221 gcgatctcgg tgccagcaag ttacagactt ttatccgcat cattattcca ctgacgatgc
     8281 cggggattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg ttctatgtgt
     8341 ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag gtacagttcc
     8401 tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact atcgtaatgg
     8461 gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataagaag gcaaccgaaa
     8521 tagatgatta gtgctgaata ttcaaagagt taatagcaaa tttggcatgc tttttacttt
//
LOCUS       DAZUCE010000208         8573 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZUCE010000208 DAZUCE010000000
VERSION     DAZUCE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.208"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(250..513)
                     /gene="rpsT"
                     /locus_tag="ACHY03_004076"
     CDS             complement(250..513)
                     /gene="rpsT"
                     /locus_tag="ACHY03_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFS3907251.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            616..834
                     /locus_tag="ACHY03_004077"
     CDS             616..834
                     /locus_tag="ACHY03_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFS3907252.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            842..1783
                     /gene="ribF"
                     /locus_tag="ACHY03_004078"
     CDS             842..1783
                     /gene="ribF"
                     /locus_tag="ACHY03_004078"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFS3907253.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFAIDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1826..4642
                     /gene="ileS"
                     /locus_tag="ACHY03_004079"
     CDS             1826..4642
                     /gene="ileS"
                     /locus_tag="ACHY03_004079"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFS3907254.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4642..5136
                     /gene="lspA"
                     /locus_tag="ACHY03_004080"
     CDS             4642..5136
                     /gene="lspA"
                     /locus_tag="ACHY03_004080"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFS3907255.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5224..5673
                     /gene="fkpB"
                     /locus_tag="ACHY03_004081"
     CDS             5224..5673
                     /gene="fkpB"
                     /locus_tag="ACHY03_004081"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS3907256.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5675..6625
                     /gene="ispH"
                     /locus_tag="ACHY03_004082"
                     /gene_synonym="lytB"
     CDS             5675..6625
                     /gene="ispH"
                     /locus_tag="ACHY03_004082"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFS3907257.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6691..7605
                     /gene="rihC"
                     /locus_tag="ACHY03_004083"
     CDS             6691..7605
                     /gene="rihC"
                     /locus_tag="ACHY03_004083"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFS3907258.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7772..>8573
                     /gene="dapB"
                     /locus_tag="ACHY03_004084"
     CDS             7772..>8573
                     /gene="dapB"
                     /locus_tag="ACHY03_004084"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFS3907259.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDV"
BASE COUNT         1943 a         2143 c         2449 g         2038 t
ORIGIN      
        1 cctcaatgtg tatcacaata tccatattct ttgtggggga gtctggagat tgagtagata
       61 ttcttgttca gaatgtatca gccgatggtt ctacgattct taagccacga agagttcaga
      121 tagtacaacg gcatgtctct tttgactatc tggcaaccgg cagtgtgttc tctcacgcat
      181 cacaaaagca gcaggcataa aaaaacccgc ttgcgcgggc tttttcacaa agcttcagca
      241 aattggcgat taagccagtt tgttgatctg tgcagtcagg ttagccttat gacgtgcagc
      301 tttgtttttg tggatcagac ctttagcagc ctgacggtcc acgatcggtt gcatttcgtt
      361 aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata gctgcgtata ctttcttgat
      421 gaaagtacgc atcatagagc gacggcttgc gttgtgctta cgagcctttt cagactgaat
      481 ggcgcgcttc ttagctgatt tgatattagc caaggtccaa ctcccaaatg tgttctatat
      541 ggacaattca aaggccgagg aatatgccct tttagccttc ttttgtcaat ggatttgtgc
      601 aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg atggcgcagg attctaccag
      661 cttgcggggt gtgaatacag cttttccgcg ataaaaattg cagcaggcgg tcagtttctt
      721 cccgtgattt gcgccatggc aatgaaaagc cacttctttc tgatttcggt actcaatcgc
      781 cggttaacct tgaccgctgt acaaggtata ctcggacgat tttcactgtt ttgagccaga
      841 catgaagctg atacgcggca tacataatct cagccaggcc ccgcaagaag ggtgtgtgct
      901 gactattggt aatttcgacg gcgtgcatcg cggtcatcgc gcgctgttac agggcttgca
      961 ggaagaaggg cgcaagcgca acttaccggt gatggtgatg ctttttgaac ctcaaccact
     1021 ggaactgttt gctatcgata aagccccggc acggctgacc cggctgcggg aaaaactgcg
     1081 ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg cgtttcgaca ggcgttttgc
     1141 ggcgttaacc gcgcaaaatt tcatcagcga tcttttggtg aagcatttgc gcgtaaaatt
     1201 tcttgccgta ggtgatgatt tccgctttgg cgctggtcgt gaaggcgatt tcttgttatt
     1261 acagaaagct ggcatggaat acggcttcga tatcaccagt acgcaaactt tttgcgaagg
     1321 tggtgtgcgt atcagcagca ccgccgtgcg tcaggcgctg gctgatgaca atctggctct
     1381 ggcagaaagt ttactggggc acccgtttgc catctccggg cgtgtagtcc acggtgatga
     1441 attagggcgc actataggtt tcccgacggc gaatgtaccg ctacgccgtc aggtttcccc
     1501 ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt gaaaagccgt tacccggcgt
     1561 ggcaaacatc ggaacacgcc caacggttgc cggtattcgc cagcagctgg aagtgcattt
     1621 gttagatgtt gcaatggacc tttacggtcg ccatatacaa gtagtgctgc gtaaaaaaat
     1681 acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa gcgcagattg cgcgtgatga
     1741 attaaccgcc cgcgaatttt ttgggctaac aaaaccggct taagcctgtt atgtaatcaa
     1801 accgaaacac ggaaccgaga atctgatgag tgactataaa tcaaccctga atttgccgga
     1861 aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa ccgggaatgc tggcgcgttg
     1921 gactgatgat gatctgtacg gcatcatccg tgcggctaaa aaaggcaaaa aaaccttcat
     1981 tctgcatgat ggccctcctt atgcgaatgg cagcattcat attggtcact cggttaacaa
     2041 gattctgaaa gacattatcg tgaagtccaa agggctttcc ggttatgact cgccgtatgt
     2101 gcctggctgg gactgccacg gtctgccgat cgagctgaaa gtagagcaag aatacggtaa
     2161 gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag tgccgcgaat acgcggcgac
     2221 ccaggttgac ggtcaacgca aagactttat ccgtctgggc gtgctgggcg attggtcgca
     2281 cccgtacctg accatggact tcaaaactga agccaatatc atccgcgcgc tgggcaaaat
     2341 catcggcaat ggtcacctgc acaaaggcgc gaagccggtg cactggtgcg tagactgccg
     2401 ttctgcactg gcagaagcgg aagttgagta ttacgacaaa acttctccgt ccattgacgt
     2461 cgctttccag gcggtcgatc aggatgcgct gaaagcgaaa tttggcgtca gcaacgttaa
     2521 cggcccaatc tcgctggtga tctggaccac tacgccgtgg actctgcctg cgaaccgcgc
     2581 aatctctatt gcacctgatt tcgactatgc gctggtgcag atcgacggtc aggccgtgat
     2641 tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc ggcgtgaccg attacaccat
     2701 tctcggcacg gtaaaaggtg cagagcttga gctgctgcgc tttacccatc cgtttatggg
     2761 cttcgacgtt ccggcaatcc tcggcgatca cgttaccctg gatgccggta ccggtgccgt
     2821 tcacaccgcg cctggccacg gcccggacga ctatgtgatc ggtcagaaat acggcctgga
     2881 aaccgctaac ccggttggcc cggacggcac ttatctgccg ggcacttatc cgacgctgga
     2941 tggtgtgaac gtcttcaaag cgaacgacat cgtcgttgcg ctgctgcagg aaaaaggcgc
     3001 gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc tgctggcgtc acaaaacgcc
     3061 gatcatcttc cgcgcgacac cgcagtggtt cgtcagcatg gatcagaaag gtctgcgtgc
     3121 gcagtcactg aaagagatca aaggcgtgca gtggatcccg gactggggcc aggcgcgtat
     3181 cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc cgtcagcgta cctggggcgt
     3241 accgatgtca ctgttcgtgc acaaagacac ggaagagctg catccgcgta ccctcgaact
     3301 aatggaagaa gtggcaaaac gcgttgaagt tgacggcatc caggcgtggt gggatcttga
     3361 tgcgaaagag atcctcggcg atgaagctga tcagtacgtg aaagtgccgg acacattgga
     3421 tgtatggttt gactccggat ctacccactc ttctgttgtt gacgtgcgtc cggaatttgc
     3481 cggtcacgca gcggacatgt atctggaagg ttctgaccag caccgtggtt ggttcatgtc
     3541 ttccctaatg atctccaccg cgatgaaggg caaagcgccg tatcgtcagg tactgaccca
     3601 cggctttacc gtggatggtc agggtcgcaa gatgtctaaa tccatcggca acaccgtttc
     3661 gccgcaggat gtgatgaaca aactgggcgc ggatattctg cgtctgtggg tggcatcaac
     3721 cgactacacc ggtgaaatgg ccgtttctga cgagatcctg aaacgtgcag ccgacagcta
     3781 tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac ctgaacggtt ttgatccggc
     3841 aaaagatatg gtgaaaccgg aagagatggt ggtactggat cgctgggccg taggttgtgc
     3901 gaaagcggca caggaagaca tcctcaaggc gtacgaagca tacgatttcc acgaagtggt
     3961 acagcgtctg atgcgcttct gctccgttga gatgggttcc ttctacctcg acatcatcaa
     4021 agaccgtcag tacaccgcca aagcggatag cgtggcgcgt cgtagctgcc agactgcgct
     4081 gtatcacatc gctgaagcgc tggtgcgctg gatggcacca atcctctcct tcaccgctga
     4141 tgaagtgtgg ggctatctgc cgggcgaacg tgaaaaatac gtcttcaccg gtgagtggta
     4201 cgaaggcctg tttggtctgg cagacagtga agcaatgaac gatgcgttct gggacgagct
     4261 gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa gcgcgtgccg acaagaaagt
     4321 gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa ccggaactgg cggcgaaact
     4381 gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc tccggcgcta ccgttgcaga
     4441 ctataacgac gcacctgctg atgctcagca gagcgaagta ctcaaaggac tgaaagtcgc
     4501 gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg cactacaccc aggatgtcgg
     4561 caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc agcaacgtcg ccggtgacgg
     4621 tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt caacagggct acgctggctg
     4681 tggctggtgg tagtcgtgct gattatcgat ctgggcagca aatacctgat cctccagaac
     4741 tttgctctgg gggatacggt cccgctgttc ccgtcgctta atctgcatta tgcgcgtaac
     4801 tatggcgcgg cgtttagttt ccttgccgat agcggcggct ggcagcgttg gttctttgcc
     4861 ggtattgcga ttggtattag cgtgatcctg gcagtgatga tgtatcgctc gaaggccacg
     4921 cagaagctaa acaatatcgc ttatgcgctg attattggcg gcgcgctggg caacctgttc
     4981 gaccgcctgt ggcacggctt cgttgtcgat atgatcgact tctacgtcgg cgactggcac
     5041 ttcgccacct tcaaccttgc cgatactgcc atttgtgtcg gtgcggcact gattgtgctg
     5101 gaaggttttt tgccttctaa agcgaaaaaa caataataaa ccctgccgga taaggcctac
     5161 agattgctgc gaaatcgtag gccgcatccg gcaaaaatcc tcaaaatata agagcaaacc
     5221 tgcatgtctg aatctgtaca gagcaatagc gccgtcctgg tgcacttcac gctaaaactc
     5281 gacgatggca ccaccgccga gtctacccgc aacaacggta aaccggcgct gttccgcctg
     5341 ggcgatgctt ctctttctga agggctggag caacacctgc tggggctgaa agtgggcgat
     5401 aaaaccacct tctcgctgga gccagatgcg gcgtttggcg tgccgtcacc ggacctgatt
     5461 cagtacttct cccgccgtga atttatggat gcaggcgagc cagaaattgg cgcaattatg
     5521 ctttttaccg caatggatgg cagtgagatg cctggcgtga tccgcgaaat taacggcgac
     5581 tccattaccg ttgatttcaa ccatccgctg gccgggcaga ccgttcattt tgatattgaa
     5641 gtgctggaaa tcgatccggc actggaggcg taacatgcag atcctgttgg ccaacccgcg
     5701 tggtttttgt gccggggtag accgcgctat cagcattgtt gaaaacgcgc tggccattta
     5761 cggcgcaccg atatatgtcc gtcacgaagt ggtacataac cgctatgtgg tcgatagctt
     5821 gcgtgagcgt ggggctatct ttattgagca gattagcgaa gtaccggacg gcgcgatcct
     5881 gattttctcc gcacacggtg tttctcaggc ggtacgtaac gaagcaaaaa gccgcgattt
     5941 gacggtgttt gatgccacct gtccgctggt gaccaaagtg catatggaag tcgcccgcgc
     6001 cagccgtcgt ggcgaagaat ctattctcat cggtcacgcc gggcacccgg aagtggaagg
     6061 gacaatgggt cagtacagca acccggaagg gggaatgtat ctggtcgaat cgccggacga
     6121 tgtgtggaaa ctgacggtca aaaacgaaga gaaactctcc tttatgaccc agaccacgct
     6181 gtcggtggat gacacgtctg atgtgatcga cgcgctgcgt aaacgcttcc cgaaaattgt
     6241 cggtccgcgc aaagatgaca tctgctacgc cacgactaac cgacaggaag cggtacgtgc
     6301 cctggcagaa caggcggaag ttgtgctggt ggtcggttcg aaaaactcct ccaactccaa
     6361 ccgtctggcg gagctggccc agcgtatggg caaacgcgcg tttttgattg acgatgcgaa
     6421 agatatccag gaagagtggg tgaaagaggt taaatgcgtc ggcgtgactg cgggcgcatc
     6481 ggctccggat attctggtgc agaatgtggt ggcacgtttg cagcagctgg gtggtggtga
     6541 agccattccg ctggaaggcc gtgaagaaaa tattgttttc gaagtgccga aagagctgcg
     6601 tgtcgatatt cgtgaagtcg attaagtcat tagcagccta agttatgcga aaatgccggt
     6661 cttgttatcg gcatttttta tggagaaaac atgcgtttac ctatcttcct cgatactgac
     6721 cccggcattg acgatgccgt cgccattgcc gccgcgattt ttgcccccga actcgacctg
     6781 caactgatga ccaccgtcgc gggtaatgtc tcggttgaga aaaccacccg caacgccctg
     6841 caactgctgc atttctggaa tgcggagatt ccgctcgccc agggagccgc tgtgccgctg
     6901 gtacgcgcac cgcgtgatgc agcatctgtg cacggcgaat cgggaatggc tggctacgac
     6961 tttgttgagc acaaccgaaa gccactcggg ataccggcgt ttctggcgat tcgggatgcc
     7021 ctgatgcgtg caccagagcc cgttaccctg gtggccatcg gcccgttaac caatattgcg
     7081 ctgttacttt cacaatgccc ggaatgtaaa ccgtatattc gccgtctggt gatcatgggt
     7141 ggttctgccg gacgcggaaa ctgtacgcca aacgccgagt ttaatattgc tgccgatcca
     7201 gaagcggctg cctgtgtctt ccgtagcggt attgaaatcg tcatgtgcgg tttggatgtc
     7261 accaatcagg caatattaac tcctgactat ctctctacac tgccgcagtt aaaccgtacc
     7321 gggaaaatgc ttcacgccct gtttagccac taccgtagcg gcagtatgca aagcggcttg
     7381 cgaatgcacg atctctgcgc catcgcctgg ctggtgcgcc cggacctgtt cactctcaaa
     7441 ccctgttttg tggcagtgga aactcagggc gaatttacct caggcacgac ggtggttgat
     7501 atcgacggtt gcctgggcaa gccagccaat gtacaggtgg cattggatct ggatgtgaaa
     7561 ggcttccagc agtgggtggc tgaggtgctg gctctggcgt cgtaacctgt cacatgttat
     7621 tggcatgcag tcattcatcg actcatgcct ttcactgata tccctcccta tttatcatta
     7681 atttctaatt atcagcgttt ttggctggcg gcgtagcgat gcgctggtta ctctgaaaac
     7741 ggtctatgca aattaacaaa agagaatagc tatgcatgat gcaaacatcc gcgttgccat
     7801 cgcgggagcc ggggggcgta tgggccgcca gttgattcag gcggcgctgg cattagaagg
     7861 cgtgcagctg ggcgcggcgc tggagcgcga aggatcttct ttactgggca gcgacgccgg
     7921 tgagctggct ggtgcaggga aaacaggcgt taccgtgcaa agcagccttg atgcgataaa
     7981 agatgatttt gatgtgttta tcgattttac ccgtccggaa ggtacgctga accatctcgc
     8041 tttttgtcgc cagcatggca aagggatggt gatcggcact acggggtttg acgaagccgg
     8101 taaacaggca attcgtgacg ccgctgccga tattgcgatt gtctttgccg ccaattttag
     8161 cgttggcgtt aacgtcatgc ttaagctgct ggagaaagca gccaaagtga tgggtgacta
     8221 caccgatatc gaaattattg aagcacatca tagacataaa gttgatgcgc cgtcaggcac
     8281 cgcactggca atgggagagg cgatcgccca cgcccttgat aaagatctga aagattgcgc
     8341 ggtctacagt cgtgaaggcc ataccggtga acgtgtgcct ggtaccattg gttttgccac
     8401 cgtgcgtgca ggtgacatcg ttggcgaaca taccgcgatg tttgccgata ttggcgagcg
     8461 tctggagatc acccataagg cgtccagccg catgacattt gctaacggtg cggtaagatc
     8521 ggctttgtgg ttgagtggta aggaaagtgg tctttttgat atgcgagatg tgc
//
LOCUS       DAZUCE010000209         8436 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZUCE010000209 DAZUCE010000000
VERSION     DAZUCE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.209"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..737
                     /gene="yneK"
                     /locus_tag="ACHY03_004085"
     CDS             <1..737
                     /gene="yneK"
                     /locus_tag="ACHY03_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFS3907260.1"
                     /translation="CAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQFPMINPGVPIKR
                     AHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDPN
                     LITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDLFLFF
                     ASMLLHYHQFMNPNESDLNNVLMPASLSDDKIIKHMARKTLKLFVKNETPPKVTHEDL
                     VKNRPRSPVRPPIPATSKTPDIPDRH"
     gene            888..2078
                     /gene="ydeA"
                     /locus_tag="ACHY03_004086"
     CDS             888..2078
                     /gene="ydeA"
                     /locus_tag="ACHY03_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFS3907261.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2103..2768)
                     /gene="marC"
                     /locus_tag="ACHY03_004087"
     CDS             complement(2103..2768)
                     /gene="marC"
                     /locus_tag="ACHY03_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFS3907262.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            2980..3414
                     /gene="marR"
                     /locus_tag="ACHY03_004088"
     CDS             2980..3414
                     /gene="marR"
                     /locus_tag="ACHY03_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFS3907263.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3434..3817
                     /gene="marA"
                     /locus_tag="ACHY03_004089"
     CDS             3434..3817
                     /gene="marA"
                     /locus_tag="ACHY03_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFS3907264.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3849..4067
                     /gene="marB"
                     /locus_tag="ACHY03_004090"
     CDS             3849..4067
                     /gene="marB"
                     /locus_tag="ACHY03_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFS3907265.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(4098..4997)
                     /gene="eamA"
                     /locus_tag="ACHY03_004091"
     CDS             complement(4098..4997)
                     /gene="eamA"
                     /locus_tag="ACHY03_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFS3907266.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSFNGQHVAMLGFILTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            5192..6379
                     /gene="ydeE"
                     /locus_tag="ACHY03_004092"
     CDS             5192..6379
                     /gene="ydeE"
                     /locus_tag="ACHY03_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFS3907267.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVDSVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGLVLTNLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            6506..6601
                     /gene="mgtS"
                     /locus_tag="ACHY03_004093"
     CDS             6506..6601
                     /gene="mgtS"
                     /locus_tag="ACHY03_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFS3907268.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            6601..6705
                     /gene="mgtT"
                     /locus_tag="ACHY03_004094"
     CDS             6601..6705
                     /gene="mgtT"
                     /locus_tag="ACHY03_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFS3907269.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(6820..7710)
                     /gene="dgcZ"
                     /locus_tag="ACHY03_004095"
     CDS             complement(6820..7710)
                     /gene="dgcZ"
                     /locus_tag="ACHY03_004095"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFS3907270.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAISHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(7965..8357)
                     /gene="ydeI"
                     /locus_tag="ACHY03_004096"
     CDS             complement(7965..8357)
                     /gene="ydeI"
                     /locus_tag="ACHY03_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFS3907271.1"
                     /translation="MKFQPIVLASFLVMPYALADDQGGLKQDAAPPPSHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         2216 a         2076 c         1874 g         2270 t
ORIGIN      
        1 tttgcgctcc caacccaaag cctgtcacta tggggcgcgt atttcagacc ggcgtcgagc
       61 gtgtgctctt tctgtttctg aatgatttta ttgaacaatt tccaatgata aaccctggcg
      121 ttcccatcaa aagagcgcat acaccacata ttgaacccct gcctccggat caccataccg
      181 ctgcagatta cttacgccag tttgatttgc ttgtcctgaa ttttatctct cgcggtaatt
      241 ttgttatact cccccgatta tggaataact ctgaggttca cagatggttc gtcaataaag
      301 atcctaactt gatcaccgcc attctcgaca taacggacag cgaattaaaa gaggatttat
      361 tgcaaagcct gatggattca ttaggttcta acaaacatgt actacccgaa gtctgcatct
      421 gctttttatc ccttttagca gaacaagaat ctcctcattt tcaggacttg tttttatttt
      481 tcgccagtat gttattgcac tatcaccaat ttatgaatcc caatgaaagt gatttgaata
      541 acgtgttgat gccagcatca ttaagtgatg ataaaattat caaacatatg gcgcgcaaga
      601 ccctcaaact ttttgtaaaa aatgaaacac cgccaaaagt tactcatgaa gacctggtga
      661 aaaacaggcc tcgctcccct gtcagaccac ctatacccgc aacctccaaa acgccagaca
      721 ttcctgatcg tcattaaacc gtgatgttta ccgtctctct gacgcgtgaa agaatcagcg
      781 tcagagaaac ggaaaacgcg atccagatca caaatgcatt gtattcacat cattaaccgt
      841 tttaagatca tttcatcact ttttcgcaac tcacccgata atctgttatg acaacaaaca
      901 ctgtttcccg caaagtggcg tggctacggg tcgttacgct ggcagtcgcc gccttcatct
      961 tcaacaccac cgaatttgtc cctgttggcc tactctctga cattgcgcaa agttttcaca
     1021 tgcaaaccgc tcaggtcggc attatgttga ccatttacgc atgggtagta gcgctaatgt
     1081 cattgccttt tatgctaatg accagccagg ttgaacggcg caaattactg atctgcctgt
     1141 ttgtggtgtt tattgccagc cacgtactgt cgtttttatc gtggagcttt accgttctgg
     1201 tgatcagtcg cattggtgtg gcttttgcac atgcgatttt ctggtcgatt acggcgtccc
     1261 tggcgatccg tatggctccg gccgggaagc gagcacaggc attgagttta attgccacag
     1321 gtacagcact ggcaatggta ttaggattac ctctcgggcg cattgtgggg cagtatttcg
     1381 gttggcgaat gaccttcttc gcgattggta tcggggcgct tgttaccctt ttgtgcctga
     1441 ttaagttact tcccttactg cccagtgagc attccggttc attgaaaagc ctcccgctat
     1501 tgttccgccg cccggcattg atgagcattt atttgttaac tgtggtggtt gtcaccgccc
     1561 attacacggc atacagctat attgagcctt ttgtgcaaaa cattgcggga ttcagcgcca
     1621 actttgccac ggcattactg ttattactcg gtggtgcggg cattattggc agcgtgattt
     1681 tcgggaaact gggtaatcag tatgcgtctg cgttggtgag tacggcgatt gcgctgttgc
     1741 tggtgtgtct ggcactgctg ctacctgcgg cgaacagtga aatacacctc ggggtgctga
     1801 gtattttctg ggggatcgcg atgatgatca tcgggcttgg tatgcaggtt aaagtgctgg
     1861 cgctggcacc agatgccacc gacgtcgcga tggcgctatt ctccgggata tttaatattg
     1921 gaatcggggc gggtgcgttg gtaggtaatc aggtgagtct gcactggtca atgtcgatga
     1981 ttggttatgt gggcgcggtg cctgcttttg ccgcgttaat atggtcaatc attatatttc
     2041 gccgctggcc agtgacactc gaagaacaga cgcaatagtt gaaaggccca ttcgggcctt
     2101 ttttaatgat acgttttaat gatttccagg atgccgttaa taataaactg cacacccata
     2161 cataccagca ggaaccccat caggcgggag atcgcttcaa tgccaccctt gcccaccagc
     2221 cgcataattg cgccggagct gcgtaggctt ccccacaaaa taaccgccac caggaaaaag
     2281 atcagcggcg gcgcaaccat cagtacccaa tcagcgaagg ttgaactctg acgcactgtg
     2341 gacgccgagc taataatcat cgcaatggtt cccggaccgg cggtacttgg cattgccagt
     2401 ggtacaaaag caatattggc actgggttca tcttccagct cttccgactt gcttttcgcc
     2461 tccggtgaat caatcgcttt ctgttgcgga aagagcatcc gaaaaccgat aaacgcaacg
     2521 attaagccgc ctgcaattcg cagaccggga atcgaaatgc caaatgtatc catcaccagt
     2581 tgcccggcgt aatacgccac catcatgatg gcaaatacgt acaccgaggc catcaacgac
     2641 tgacgattac gttcggcact gttcatgttg cctgccaggc caagaaataa cgcgacagtt
     2701 gttaatgggt tagctaacgg cagtaacacc accagcccca ggccaattgc tttaaacaaa
     2761 tctaacattg gtggttgtta tcctgtgtat ctgggttatc agcgaaaagt ataaggggta
     2821 aacaaggata aagtgtcact ctttagctaa ccttgcatcg cattgaccaa aacttgaacc
     2881 gatttagcaa aacgtggcat cggtcaattc attcatttga cttatacttg cctgggcaat
     2941 attatcccct gcaactaatt acttgccagg gcaactaatg tgaaaagtac cagcgatctg
     3001 ttcaatgaaa ttattccatt gggtcgctta atccatatgg ttaatcagaa gaaagatcgt
     3061 ctgcttaacg agtatctgtc tccgctggat attaccgcgg cacagtttaa ggtgctctgc
     3121 tctatccgct gcgcggcgtg tattactccg gttgaactga aaaaagtgtt gtcggtcgac
     3181 ctgggagcac tgacccgtat gctggatcgc ctggtctgta aaggctgggt agaaaggttg
     3241 ccgaacccga atgataagcg cggcgtactg gtaaaactta ccaccagcgg cgcggcaata
     3301 tgtgaacaat gccatcaatt agttggccag gacctgcatc aagaattaac aaaaaacctg
     3361 acggcggacg aagtggcaac acttgagcat ttgcttaaga aagtcctgcc gtaaacaaaa
     3421 aagaggtatg acgatgtcca gacgcaatac tgacgctatt accattcata gcattttgga
     3481 ctggatcgag gacaacctgg aatcgccact gtcactggag aaagtgtcag agcgttcggg
     3541 ttactctaaa tggcacctgc aacggatgtt taaaaaagaa accggtcatt cattaggcca
     3601 atacatccgc agccgtaaga tgacggaaat cgcgcaaaag ctgaaggaaa gtaacgagcc
     3661 gatactctat ctggcagaac gatatggctt cgagtcgcaa caaactctga cccgaacctt
     3721 caaaaattac tttgatgttc cgccgcataa ataccggatg accaatatgc agggcgaatc
     3781 gcgcttttta catccattaa accattacaa cagctagttg aaaacgtgac aacgtcactg
     3841 aggcaattat gaaaccgctt ttatccgcaa tagcagctgc gcttattctc ttttccgcgc
     3901 agggcgttgc ggaacaaacc cagcaaccgc tcgttacttc ctgtggcgat gtggtggttg
     3961 ttcccccatc gcaggaacaa ccaccgttcg atttaaatca catgggtaca ggcagtgaca
     4021 aatcggatgc gctgggcgtg ccctattata accaacaagc catgtagtat attcaggccc
     4081 cggcatatcg gggcttgtta acctcttacc ttcaccgctt tacgccactg caaaccaaat
     4141 acattgatat acagtccggc cataatgagc acagccccta agaattgcag acctgttaag
     4201 cgttcatcca gcagtaacgc tgcacttgcc agaccgacta ccggcaccag taatgataat
     4261 ggtgcgacac gccatgtttc atagcgtcca agcagcgtac cccaaatccc gtagccaact
     4321 atcgtcgcta caaatgccag atacatcaac gccagaatgg tggtcatgtc gatagtgacc
     4381 agactgtgaa tcatttttcc ggagccatcg agaatgagcg aggcgacaaa aaacggaatt
     4441 atcgggatta atgcgctcca gacgaccaga gacattaccg ccggacggga tgaatgagac
     4501 atgatctttt tgttgaagat attgccactc gcccaactaa atgccgctgc cagagtcaaa
     4561 ataaaaccga gcatcgccac atgctgaccg ttaaagctat cttcgattaa caccagtacg
     4621 ccaaaaatcg ctaacgcaat ccccgccagt tgcttaccgt gcagccgttc gccaaaagta
     4681 aaagcaccga gcacgatagt aaaaaacgcc tgagcctgta agaccagcga ggccagtcca
     4741 gccggcatac cgaagttaat ggcgcagaag agaaaggcaa actgtgcaaa actgatggtt
     4801 aaaccatacc ccagcagtaa attcagcggt acttttggtc gtgccacgaa aaagattgcc
     4861 gggaaggcga ccagcataaa gcgcaaaccg gccagcatca gcggtggcat gttatgaagc
     4921 cccactttga tgaccacaaa atttagcccc catacgacca ctaccagtag cgccaacacc
     4981 ccatcttttc gcgacattct accgcctctg aatttcatct tttgtaagca atcaacttag
     5041 ctgaatttac ttttctttaa cagttgattc gttagtcgcc ggttacgacg gcattaatgc
     5101 gcaaataagt cgctatactt cggatttttg ccatgctatt tctttacatc tctaaaacaa
     5161 aacataacga aacgcactgc cggacagaca aatgaactta tccctacgac gctctaccag
     5221 cgcccttctt gcctcgtcgt tgttattaac catcggacgc ggcgctacgc tgccatttat
     5281 gaccatttac ttaagtcgtc aatacagcct gagtgtcgat ctcattggtt atgcgatgac
     5341 aattgcgctc actattggcg tcgtttttag cctcggtttt ggtatcctgg cggataagtt
     5401 cgacaagaaa cgctatatgt tactggcaat tacggccttc gccagcggtt ttattgctat
     5461 tcccctggtt gacagcgtca cgctggtggt actttttttc gcactcatca actgcgccta
     5521 ttccgttttt gccacagtgt tgaaagcctg gtttgccgac aatctttcat ccaccagcaa
     5581 aacgaagatc ttctcgatca actacaccat gctcaatatt ggctggaccg tcggcccgcc
     5641 actcggcacg ctgttggtca tgcagagcat caatctgccc ttttggctgg ccgctatctg
     5701 ctctgcgttt cccatgtttt tcattcaaat ttgggtaaag cgcagcgaga aaatcatcgc
     5761 cacggaaaca ggcagtgtct ggtcgccgaa agttttatta caagataagg cactgttgtg
     5821 gtttacctgc tctggctttc tggcttcttt tgttagtggc gcatttgctt catgcatttc
     5881 acaatatgtg atggtgattg ctgatggtga ttttgccgaa aaggtggtcg cggttgttct
     5941 tccggtgaat gccgccatgg tagttacgtt gcaatattcc gtgggccgcc gacttaaccc
     6001 ggctaacatc cgcgcgctga tgacagcagg caccctctgt ttcgtcatcg gtctggtcgg
     6061 ttttattttt tccggaaaca gcctgctatt gtggggtatg tcagctgcgg tatttactgt
     6121 cggtgaaata atttatgcgc cgggcgagta tatgttgatt gaccatattg cgccgccagg
     6181 aatgaaagcc agctattttt ccgcccagtc tttaggctgg cttggtgccg caattaaccc
     6241 attagtgagt ggcctagtgc taaccaacct gccgccttcc tcgctgttta tcatcttagc
     6301 gttggtgatc attgctgcgt gggtgctgat gttaaaaggg attcgtgcaa aaccgtgggg
     6361 gcagcccgcg ctttgttgat ttaagtcgaa cacaataaaa atttaattca gccttcgttt
     6421 aggttacctc tgctaatatc tttctcattg agatgaaaat taaggtaagc gaggaaacac
     6481 accacaccat aaacggaggc aaataatgct gggtaatatg aatgttttta tggccgtact
     6541 gggaataatt ttattttctg gttttctggc cgcgtatttc agccacaaat gggatgacta
     6601 atgaacggag ataatccctc acctaaccgg ccccttgtta cagttgtgta caaggggcct
     6661 gatttttatg acggcgaaaa aaaaccgcca gtaaaccggc ggtgaatgct tgcatggata
     6721 gatttgtgtt ttgcttttac gctaacaggc attttcctgc actgataacg gatcgttgac
     6781 acagtagcat cagttttctc aatgaatgtt aaacggagct taaactcggt taatcacatt
     6841 ttgttcgtca ataaacatgc agcgatttct tccggtttgc ttaccctcat acattgcccg
     6901 gtctgctctt ccaatgacca catccagagg ctcttcagga aatgcgcgac tcacacctgc
     6961 tgtcacggta atgttgatat gcccttcaga atgtgagatg gcatggttat cgactaactg
     7021 gcaaattctg acacctgcac gacatgcttc ttcatcatta gccgctttga caataatgat
     7081 aaattcttcg cccccgtagc gataaaccgt ttcgtaatca cgcgtccaac tggctaagta
     7141 agttgccagg gtgcgtaata ctacatcgcc gattaaatgc ccgtaggtat cattaaccaa
     7201 tttaaatcgg tcaatatcca acaacattaa ataaagattc agaggctcag cgttgcgtaa
     7261 ctgatgatca aaggattcat caagaacccg acgccccggc aatcccgtca aaacatccat
     7321 attgctacgg atcgtcagca aataaatttt gtaatcggtt aatgccgcag taaaagaaag
     7381 caacccctcc tgaaaggcgt cgaaatgcgc gtcctgccag tgattttcaa caatagccag
     7441 cattaattcc cgaccacagt tatgcatatg ttgatgggca gaatccatta gccgaacgta
     7501 aggtaattca tcgttatcga gtggccccag atgatcaatc caccgaccaa actggcacag
     7561 tccataagaa tggttatccg ttatttctgg cttactggca tctctcgcga ccacgctgtg
     7621 aaacatactc accagccact ggtagtgggc atcgatagcc ttattgagat ttaacaagat
     7681 ggcatcaatt tccgttgtct tcttgatcat tgccactcct ttttcacagt tccttgtgcg
     7741 cgctattcta acgagagaaa agcaaaatta cgtcaatatt ttcatagaaa tccgaagtta
     7801 tgattcatct ctgagataac attgtgattt aaaacaaaat cagcggataa aaaagtgttt
     7861 aattctgtaa attacctctg cattatcgta aataaaagga tgacaaaaag cataacccaa
     7921 taccctaatg gcccagtagt tcaggccatc gggctaattt atttttattt ctgcaaatga
     7981 gtgacccgaa cgacggccgg cgcgcttttc ttatccagac tgccgctaat gttgatcatc
     8041 tggtccggct gaacttctcg tccatcaaaa acggccgcag gaataacaac attaatttca
     8101 ccgctcttat cgcgaaaaac gtaacggtct tctcctttgt gagaaatcaa attaccgcgt
     8161 agtgaaaccg aagcgccatc gtgcatggtt tttgcgaaat caacggtcat tttttttgca
     8221 tcatcggttc cgcgatagcc atcttctatt gcatgagacg gcggtggcgc tgcatcctgt
     8281 tttaaaccgc cctggtcatc tgccaacgca taaggcatga caagaaaact tgctaataca
     8341 atgggctgaa atttcatact gactccttaa ttgcgtttgg tttgacttat taagtctggt
     8401 tgctattttt ataattgcca aataagaata ctgcca
//
LOCUS       DAZUCE010000210         8429 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZUCE010000210 DAZUCE010000000
VERSION     DAZUCE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.210"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..169)
                     /locus_tag="ACHY03_004097"
     CDS             complement(<1..169)
                     /locus_tag="ACHY03_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS3907272.1"
                     /translation="MAFKHYDVVRAASPSDLADTLAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDV"
     gene            complement(684..1742)
                     /locus_tag="ACHY03_004098"
     CDS             complement(684..1742)
                     /locus_tag="ACHY03_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS3907273.1"
                     /translation="MANTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYAELQSEYKYLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1893..2090)
                     /locus_tag="ACHY03_004099"
     CDS             complement(1893..2090)
                     /locus_tag="ACHY03_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS3907274.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(2307..2867)
                     /locus_tag="ACHY03_004100"
     CDS             complement(2307..2867)
                     /locus_tag="ACHY03_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFS3907275.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRRMKKSGLDKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKLKMAERLNADHPEWTLVTCRRRIDQWL
                     GISEFMLHAPMRMAFVTEKKMLQTDQ"
     gene            complement(2876..3235)
                     /locus_tag="ACHY03_004101"
     CDS             complement(2876..3235)
                     /locus_tag="ACHY03_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS3907276.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVRGQ
                     LVLGGRLGVKIYKIESE"
     gene            complement(3248..4297)
                     /locus_tag="ACHY03_004102"
     CDS             complement(3248..4297)
                     /locus_tag="ACHY03_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS3907277.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWSHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            complement(4299..4577)
                     /locus_tag="ACHY03_004103"
     CDS             complement(4299..4577)
                     /locus_tag="ACHY03_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907278.1"
                     /translation="MAQDTKLHNSDNSTAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            4713..4970
                     /locus_tag="ACHY03_004104"
     CDS             4713..4970
                     /locus_tag="ACHY03_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFS3907279.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRVAIRSGK"
     gene            4976..5275
                     /locus_tag="ACHY03_004105"
     CDS             4976..5275
                     /locus_tag="ACHY03_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3907280.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNIIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGRKVFIVGIYHGGQSYETDFL"
     gene            complement(5478..5831)
                     /locus_tag="ACHY03_004106"
     CDS             complement(5478..5831)
                     /locus_tag="ACHY03_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFS3907281.1"
                     /translation="MINRIKLEHILEYARQQKHTGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPPVGVDVLLFVSSTGEQFTGFNLDNTGDFQYAQCFGAPIVCEA
                     THWMPLPEPPKEVRQ"
     gene            complement(5828..6181)
                     /locus_tag="ACHY03_004107"
     CDS             complement(5828..6181)
                     /locus_tag="ACHY03_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907282.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAKP
                     VASCIIEDGGMCVDGFGEYVGHSLPDGTHQLYAAPPVPSKLPREYRNGWPLAYSDYAE
                     GWNDCREAMLQGDKS"
     gene            complement(6183..6527)
                     /locus_tag="ACHY03_004108"
     CDS             complement(6183..6527)
                     /locus_tag="ACHY03_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS3907283.1"
                     /translation="MSKIDYQALREAAQNYRSMLAWYQEKPDSPNAEQDCDAALAAFK
                     REIRHREVDIIADLLDELEEAKQRINEQESRIVKLPEPFKLAKSSSGLTYYYADEVNA
                     ALTAAGIRIEGE"
     gene            complement(6514..6843)
                     /locus_tag="ACHY03_004109"
     CDS             complement(6514..6843)
                     /locus_tag="ACHY03_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907284.1"
                     /translation="MRASISYVDDCHLSVRVDEIVSSVPTFPTKNAAVNAGAPFGWRT
                     AVRIERRFENVWVVGKKCFQSDRSAGLNFEAYRFPFLRWEKEGGITKCPILSVRRFKQ
                     EATSEQD"
     gene            complement(6840..7490)
                     /locus_tag="ACHY03_004110"
     CDS             complement(6840..7490)
                     /locus_tag="ACHY03_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFS3907285.1"
                     /translation="MRKINYQTLREIAKLATQGEWVAFISPGTDTYAVHTPGDERCGD
                     IIKWPGFDDQKNAENNAEFIAAFNPEVVQALLDEREAQSKRIAELEANLVALAAENAL
                     ARKAVQAFCDVVGDNTEVICEEVGRDGVLVILEAMKATGNMPATDAFLSEVRALGVDA
                     AIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLIGKVEMTEWLVDFAAQLRKGVQS"
     gene            complement(7477..7782)
                     /locus_tag="ACHY03_004111"
     CDS             complement(7477..7782)
                     /locus_tag="ACHY03_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFS3907286.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSEKN"
     gene            complement(7779..8060)
                     /locus_tag="ACHY03_004112"
     CDS             complement(7779..8060)
                     /locus_tag="ACHY03_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3907287.1"
                     /translation="MILRQCAGTMKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGVPRREIAEKFGMKLRAVNNYVYFDRRIQA"
     gene            complement(8093..>8429)
                     /locus_tag="ACHY03_004113"
     CDS             complement(8093..>8429)
                     /locus_tag="ACHY03_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS3907288.1"
                     /translation="DLPAEPEAASVAETDGKAFPQPAGVALPVREAETQEEIKTESVA
                     VTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALRELNKCRDILRD
                     ITATREQQR"
BASE COUNT         1963 a         2395 c         1897 g         2174 t
ORIGIN      
        1 tcacatcacc ttctgcgaca atcgcctgaa taagtgctgc gccatcatcc gtatacgaag
       61 aaaacggccc gccgtatggc tgccatcctt cacgaatttt ttgagcaagt gtatcagcaa
      121 ggtctgacgg cgacgccgcc ctgaccacgt catagtgttt aaatgccatg aatcctcccg
      181 gccgggataa tgttgtgagt cagataaggg gcgggctgaa gtccggaagt tacaggacaa
      241 tgacagaagg aagactacag cccgcaatac gaaaaaggcc gcgcagttgc gcagagtgat
      301 tactgtcggg tattattcgc cagctgaaaa attgattact tcacgttttg ttgtttattc
      361 cttgctgccc gcgtctcccg gcgcgggctt tttttgcatg taaaaaggct cctgcgatga
      421 ggagcctgga tatatgccta atctctgtat acagcatgat gccgggtgcc tcccggtgaa
      481 ttctgcaatg accagacaga atccgcaact tgcctataca atacgcaacc aaacatctgt
      541 cattatgccc ctccgctcag ggggattcac catgcctgtt ccttttaaca aacgccccgc
      601 aaaacagaca actgtcaacc gtctgaattg tgagacattt aaaattttcg gggcgtaact
      661 gatacccggt taattctgca atatcatccg ttctgactga ctaaatcctg aacttccctg
      721 acagtctgct caaaacgttc agtctccagc tcaacgccag ttgcacgacg tcccagcgcc
      781 atcgctgctt tgactgtcga acccgacccc atgaagaaat ctgcaaccag gtcacccgga
      841 cgactgctcg cgctgattat ctgctgcagc atttctgctg gtttttcgca cggatgtttc
      901 ccgggatagt actgcaccgg tttatgcgtc cacacatcgg tgtacggcac ctgcgccgtc
      961 acgccaaaat accgccgcag atacttatat tcactctgca gttccgcata ctgccggttc
     1021 agttcactgt atgtgctgac cagttggtga tgtggctttt ccagttctcc gcgctgatgc
     1081 ttctcttctg ccacccgggc aaacagcgcc tgtaatttca gataatcgct ttcgtccggt
     1141 agctgccact ggctggcact gaaccagtgc gacaccatgt ttttctttcc tgtggcatca
     1201 gcaatctgtt ttgccgttat ccccagggca gcacgtgcat cacgaaagta agaaatcagc
     1261 ggggccatca catgctgttt cagtgccctg cccttcgcct catacccggc atctttcggg
     1321 cgatacggcc cctgataatg ttccgcgaac agaatgcgct ctgtggcggg gaaatacgcc
     1381 cgcaggcttt ccttgttgca cccgttccag cgtccggacg gcttcgccca gataatatgg
     1441 ttcagcacac tgaagcgttc acgcatcatg atttcaatgt cagacgccag gcgatgacca
     1501 cagaacaggt aaagacttcc ggcaggtttc agcacccgcc agaactgcgc aagacactgg
     1561 tccagccatt tcaggtaatc ctcatccccc gtccactggt tatcccagcc ctcgggcttc
     1621 actttaaagt atggcgggtc tgtgactatc agatcgacag agttttccgg taaggtctgg
     1681 ataaattcca ggcaatcagc gttgattagc tcacaactgg atatttttac agtattagcc
     1741 atagatcaat aagcacttct ctgataggct cattctgctt ttgcgcaaag cagtgggcct
     1801 gaggtttgct tgtgatccgg acgcatgagc agatggctgg tgagtgcccc taacacccac
     1861 cagccgccca tttaccacaa ataaaaaagc cttcaggact gaaggcgtct gtaacaaccg
     1921 aactgatagt ctgccagacc cgccataaca agctgggtca gtattaactg acagcgttcg
     1981 cgtgaaaggt acgtattctg tgcaatctcc ccgactgtcg ccggttcggt gacgcttaat
     2041 tcattaaaca ccactctggc ggtttctgtc atatcctgct gtttcagcat gtcttttccc
     2101 cttttccggt taacgtgaca caccaataac tcttgtcgaa aaagccagca agctgaaaga
     2161 acggtattaa taaccaccag cgaatttatt gcgctgctgt atattacgga cacaaaaaaa
     2221 ccaccttccg gtggcttcct tgtgcgaaaa aacttgcgtt tcgcctcgcg atacagcctt
     2281 gcgaagctta tacggattga agcagtttat tgatcagttt gcaacatttt tttctctgta
     2341 acaaaagcca tacgcatggg ggcatgtaac ataaattcag atatccccaa ccactgatca
     2401 attcggcgtc tgcacgtaac caacgtccac tcaggatgat ctgcatttaa cctttcggcc
     2461 attttcagtt tgctcattcc ccgtccttcg tacctttgcc ggagaatatt gattagtccg
     2521 ggatattccc caagcacctc actgataacg cgatcaataa tcaacgcctc tgtgtctgta
     2581 caatgtgaca accagctctt ctgcttccct ctggtcatat cccgaaaaaa tgcctcaagt
     2641 tccggtttat ccagcccgga tttcttcatt ctgcgtaaaa cttcattaac tgctgttttc
     2701 gtcagctttt tcgaaaccag taaccggtta aacatatttc cggatttacc cccaccgata
     2761 tacgaccacc gcccccacat ccgtaatttc ccctggatcc agactgcttc cagcgtgttc
     2821 aggcgtaaat gttcgccgct tttgcctgta atttccggat atatcatatt tacgctcact
     2881 cactctcaat tttgtaaatc ttcacaccca gccgaccacc aagaacaagc tgaccacgta
     2941 caatattgat ttcatcaaac tgctcgtcat caatgagcac tcccgcatgc gtcagcgcat
     3001 ccagcggtgc tttcagaatg ttgtccaggt cacgacgacg cttatccggt ggctctgcaa
     3061 taatttttat tgccagccgt ccggacaggc ttaatttcag ccgctgctgg cgaacaataa
     3121 gcgccactgc acggcgataa cgctccccgg cttttgatac aaaatatgtg ctgccacggc
     3181 gtcgccagta agtgttcacc gtcggcgggt aaggtaaaac caaatctatg agcatcagtc
     3241 acctctttta cccgagcacg ccagtcgcaa aggcgtgatc aagaaagcga aaaattaact
     3301 caatctgaga gccgtacttt ttctcaaact ccagcgggtc tgcatgaagt tcgttgtggt
     3361 gctcccggca caacggtagc gtgaaaatat cgtgggcctt tgtccccatt cccccctgac
     3421 catgaccaat caggtgatgc ggatcgtcag caggcttacc acaacacgca cacggctgtg
     3481 tctttaccca gcgcgtgtat ttctcattaa cccaacggcg acgtttaggc cgcctcatga
     3541 acgattcagg agactccgga tcaacggcga tactgacaac cgtttttttc tgtggtggat
     3601 tttgttgctg gtggacgtga agtggcagcg caatattttt tgtgcgctgc ttcagtatgc
     3661 tgatggctgt ctgttctccc ggtacgatgt cactctcacg gtatacggag cggatttttt
     3721 ccgctggtaa tcccagcgaa cgacgcgcta ctgcctcagg tagtgcatcc accacctgat
     3781 tgcaggccgc ccaccaggat aattcggcca gcgataactc cctctcctgc gtgccgctta
     3841 ttgcgtgacg gatgacgtca atcatccagg caaccagatt ctgctgagca agttgatcga
     3901 gtgattctga tgtctggtcg cgcagctggt tgtcacagtg ccagcacaac accatcgcgc
     3961 cggtaccgta acggtgaatg actgtttcgc tgtgatgata atcgccgtgt gaccactggc
     4021 aggatttcac gtgacgtaac aaccagtcag acagtgcacc tgcaccacct gctgcacgaa
     4081 taacccgctc atcgctgaaa aatggcagta atgttttatc ctctgccagc ggctggcgaa
     4141 cggcaggaac gactccggac ggcagaccgc gcatgttttt cggttccggt tccaccaata
     4201 ttctgccgtt atggaatgct gacattgatt cacggcccgg cttaaggagc accatcccga
     4261 gttccggtac cagaacaggt cgaagtaata cccgcacgtt acctccagat gcgctgctgg
     4321 aatgtgcggg acggacgcgg tgggcgttcg gaataaggga gcctgacgga gattatccag
     4381 tgacgacgat cgaagctgag atctttctga aactcgtaac cacgtctgcg gtagcactgg
     4441 atcagccatt cggcctgttc ttcagtgcat gggtcatgct ggaaccagtc agatttgaat
     4501 gcatgagaac gccgcccgtg cctgctggca ggggcggtgg agttatccga attgtgtaat
     4561 ttggtatctt gcgccatcgg ttgtctctgc tggcgcagca ggtgccagtt gttcaggctg
     4621 gcgtgcggca atattgtctc tgatttctgt tgtcgtcaac aggcagcgtg ctatcatcga
     4681 atagtgttct atcctactcc gtgaggttta ccatgcgtac aacccaacaa ttcagcatta
     4741 cattaactaa cgagatggct gacatggtgc gcgcccgtgt ggcttccggc gcctatgctt
     4801 cagaaagcga ggtcattcgt gaagggcttc gcgcactgaa tgagcgcgat aaagcaatcg
     4861 aagcgtggtt aacgcattca gccgccccct ctcttgattc tatccgcgaa aacccaaaca
     4921 acggacgctc catttcacag gttcgcgtcg cgattcgatc cgggaagtaa tctgcatgac
     4981 atatgaagtc atcattactc ctgaggccga acaacaaata atcaacctgc acagatatat
     5041 aacggagaaa gcagggaaca tcattgctga caattatgcc aatgcgcttc ttgattatct
     5101 tgatgggttt tctacattcc cgcatcgggg caataaacgc gatgatattc gccaggggat
     5161 gcgggtaact catttccgcc acagaacgat tattgctttt gccgttgatg gcagaaaagt
     5221 ctttattgtc ggtatctatc atggtgggca aagttatgaa accgatttct tataaacttt
     5281 tacccacatc attccggtgt tagaataaac cgtctgcccc tctcttactg cggattcgta
     5341 ggctatataa atcaaagatc ccggctcatg tttgtggcgg gatctttttt cggcgattta
     5401 tccccagcgg caaatcgaat acaccaccag cgccaccgcc attgcaatac caacatttga
     5461 gaaggcctca ggccagctca ttggcgcacc tccttcggcg gttctggtag cggcatccag
     5521 tgtgtggctt cacacacgat aggcgcaccg aaacactgcg catactggaa gtcacccgta
     5581 ttatccaggt taaaacctgt aaactgttca cccgtgctgg acacaaacag tagtacatca
     5641 acaccaactg gcggcattcg ctcactacag cttatccaac catccggagt taccggagaa
     5701 ttgccagcct tgcgcatggc aatctccatg atttcaacca tatcccctgg tggaatttta
     5761 caatgctgac cagtatgctt ctgctgcctg gcatattcga ggatgtgctc cagcttgata
     5821 cgattaatca tgatttatct ccctgaagca tggcttcgcg gcagtcgttc cagccttcag
     5881 cataatcact atatgcaaga ggccaaccgt ttctgtattc acgcggcaac ttactaggca
     5941 ctggcggggc ggcgtaaagc tggtgcgttc catcaggcag cgagtgacca acatactcac
     6001 cgaacccgtc aacacacatg cccccatctt caataataca tgaagctaca ggcttcgctt
     6061 ccagcgatgc cagcgcgata agcgccagct cacgaatttc accgccgtct atatcgtcaa
     6121 tgtcatcgcg gccagaaatg ttagccagcc attgcagtcg ctctttggta atagtggtca
     6181 tctcactctc cttctatgcg aataccagca gcagtcagtg cggcattgac ctcatcagcg
     6241 tagtagtacg ttaatccact tgatgattta gccagcttga atggttctgg taacttcact
     6301 atgcgggact cctgctcgtt gatacgctgt tttgcttcct ccagttcatc cagcaaatca
     6361 gcgataatat ccacttcacg atgacggatt tcacgcttaa acgcagccaa agctgcatca
     6421 caatcctgct cagcgtttgg actgtctggt ttctcctgat accacgccag catcgaccga
     6481 tagttttgtg ctgcctcacg caatgcctga tagtcaatct tgctcactgg ttgcctcctg
     6541 cttgaatctg cgcacggaaa gaatcgggca ttttgtaatc ccgccctctt tctcccatcg
     6601 gaggaacggg aatcgatatg cttcaaaatt aagccccgca gagcgatcgc tttggaagca
     6661 tttcttacct accacccaaa cgttttcgaa tcgacgctca atacgaactg ccgtcctcca
     6721 tccgaatggc gcgcctgcat taacggctgc attttttgtc ggaaaagttg gcacggatga
     6781 cacaatttca tcaacacgca cgcttaggtg gcaatcgtcg acataactaa ttgaagctct
     6841 catgactgaa ctcctttgcg aagctgggca gcaaagtcaa ctaaccactc agtcatttca
     6901 accttcccta tcaggtctga accagggtgc atacagcaat cactctgcgc cgctttgaaa
     6961 tccttatact catattcttg ggccaccaga ttttttgcag cttctatagc agcatccacc
     7021 cccagcgccc gcacttcaga caggaaagca tcggtggctg gcatatttcc tgttgccttc
     7081 atggcctcca aaataaccag aacgccatct ctcccaacct cctcgcagat aacctcggtg
     7141 ttgtcgccaa caacatcgca gaatgcctga actgctttac gagcaagtgc attctccgcc
     7201 gccagcgcca caagattagc ttccagctct gcaatacgtt tgctttgggc ttcccgttca
     7261 tccagcagtg cctgtactac ttcagggttg aaagctgcaa taaattctgc gttgttctct
     7321 gcatttttct ggtcatcaaa gccaggccat ttgataatgt ctccgcaacg ttcatccccc
     7381 ggcgtatgca ccgcatatgt atcagtgccc ggcgaaatga atgcgaccca ttcgccctgg
     7441 gttgccagtt ttgctatctc acgtagcgtc tgataattaa tttttctcac tggttgcctc
     7501 ctggaaaata accgcatgtc ccagtttctc cgccagtgcc agttctgcct tagcgcctgc
     7561 cgaccgctgc cagcctttca gcatgtaaat tgcatccaca caacgaatca ttgccatgca
     7621 aatatccatg taatgtggct gagtcagccc gtccggaagt actgccgggt ttaaaacggt
     7681 atgcccttcc cgtttcagcg cttcttccgc cctgtgaaac gcctcgcggt tgaaattttc
     7741 atatcccgtc atcggaccgg cgatataaat tctcaccctc acgcctgaat cctcctgtcg
     7801 aaataaacgt agttattcac tgcacgcaac ttcattccga acttttcggc aatttcccgt
     7861 cggggtacac cgcgctgatg cagttgccgc gccagctcaa tatcacgctg tgaatatttt
     7921 gccgacgggt gaaaatcacc acgtaacatc atactgatgc ccagctcccg tgccttcgtt
     7981 ctcactgccg cttcagttcg tccgataagt gcgccaatgc ttttcacctt catagttccc
     8041 gcacactggc ggagtatcag gatttcagcc cggcaccacg tcttccagcc actcaccgct
     8101 gctgttctct ggtggcggta atatcccgga gaatatcccg gcacttgttc agctcccgta
     8161 gcgcggcgca gactcgctcc cacttctgaa cctgaccttt tgcccggcgc agctcgcggt
     8221 tagccacatg cagcgatggt aaaatcagac catccggatg ctttctggtg aacgacggct
     8281 gtgactgcac tgtgaccgcc acactttccg tttttatttc ttcctgtgtt tccgcttccc
     8341 gaactggtaa cgcaacacct gctggctgag gaaaggcttt accatcggtt tccgctacgg
     8401 atgcagcttc cggctctgcc ggtaaatca
//
LOCUS       DAZUCE010000211         8415 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZUCE010000211 DAZUCE010000000
VERSION     DAZUCE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.211"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..375
                     /locus_tag="ACHY03_004114"
     CDS             <1..375
                     /locus_tag="ACHY03_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907289.1"
                     /translation="ENAWHLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRNDNIFIL
                     FSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEILPVNLTPETLFGSKSQD
                     KHALFPIFTESWRANRIKNKGI"
     gene            598..1095
                     /gene="ubiC"
                     /locus_tag="ACHY03_004115"
     CDS             598..1095
                     /gene="ubiC"
                     /locus_tag="ACHY03_004115"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFS3907290.1"
                     /translation="MSHPALTQLRALRYFTEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1108..1980
                     /gene="ubiA"
                     /locus_tag="ACHY03_004116"
     CDS             1108..1980
                     /gene="ubiA"
                     /locus_tag="ACHY03_004116"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFS3907291.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2135..4558)
                     /gene="plsB"
                     /locus_tag="ACHY03_004117"
     CDS             complement(2135..4558)
                     /gene="plsB"
                     /locus_tag="ACHY03_004117"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFS3907292.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4729..5097
                     /gene="dgkA"
                     /locus_tag="ACHY03_004118"
     CDS             4729..5097
                     /gene="dgkA"
                     /locus_tag="ACHY03_004118"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFS3907293.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5207..5815
                     /gene="lexA"
                     /locus_tag="ACHY03_004119"
     CDS             5207..5815
                     /gene="lexA"
                     /locus_tag="ACHY03_004119"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFS3907294.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5888..7213
                     /gene="dinF"
                     /locus_tag="ACHY03_004120"
     CDS             5888..7213
                     /gene="dinF"
                     /locus_tag="ACHY03_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFS3907295.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7329..7538
                     /gene="yjbJ"
                     /locus_tag="ACHY03_004121"
     CDS             7329..7538
                     /gene="yjbJ"
                     /locus_tag="ACHY03_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFS3907296.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7580..8095)
                     /gene="zur"
                     /locus_tag="ACHY03_004122"
     CDS             complement(7580..8095)
                     /gene="zur"
                     /locus_tag="ACHY03_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFS3907297.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1920 a         2048 c         2290 g         2157 t
ORIGIN      
        1 gaaaatgcct ggcatcttta taaagagtgg attcgtagtg atgatgtttc acctttattt
       61 atagaaattg aagataattt aagaaccttt aacacgaatg aattaacacg aaacgataat
      121 atctttatct tgttctcctc tgtcgatgat gggccagtta tggtggtaag ctcccagcgc
      181 ttacatgata tgttgaatcc tacaaaagat accaattgga attccacgta tatctacaaa
      241 tccagacatg agatattgcc tgttaatctt actccggaaa cacttttcgg ctccaaatct
      301 caggataaac atgcactttt ccccattttt actgagagtt ggcgagctaa tcgtataaag
      361 aataaaggta tttaagtaaa ggaaaacatc accgttcctg gcatcctggg cggtgatgcc
      421 cctatggttg ccctcgccag cacgggcatc ggcaaagcgt aaggttcaac agcgttttac
      481 cacttcatgc gattgttgcg tttttgttgc gcattagatc acttaatttg ctttacatct
      541 cccgtaaaca cttttctgcg atacaatgcc tttacgttat gtaacggaga gttcggcatg
      601 tcacaccccg cgttaacgca actgcgtgcg ctgcgctatt ttacagagat ccctgccctg
      661 gatccgcaac tgctcgactg gctgttgctg gaagattcca tgacaaaacg ttttgaacag
      721 cagggaaaaa cggtgagcgt gacgatgatc cgcgaagggt ttgtcgagca gaatgaaatc
      781 cccgaagaac tgccgctgct gccgaaagag tctcgttact ggttacgtga aattttgtta
      841 tgtgccgatg gtgaaccgtg gcttgccggt cgtaccgtcg ttcctgtgtc aacgttaagc
      901 gggccggagc tggcgttaca aaaattgggt aaaacgccgt taggacgcta tctgttcaca
      961 tcatcgacat taacccggga ctttattgag ataggccgtg atgccggact gtgggggcga
     1021 cgttcccgcc tgcgattaag cggtaaaccg ctgttgctaa cagaactgtt tttaccggcg
     1081 tcaccgttgt actaagagga aaaaaatatg gagtggagtc tgacgcagaa taagctgctg
     1141 gcgtttcatc gcttaatgcg tacggataag ccaattggcg cgttactgct gctctggcca
     1201 acattatggg cgttgtgggt ggcgacaccg ggcgttcccc agctctggat cctggcggtg
     1261 tttgtcgcgg gtgtctggct gatgcgcgct gccggatgtg tggtgaatga ttatgctgac
     1321 cgcaagtttg atggtcatgt taagcgcacg gcgaaccgac cacttcccag cggcgcggta
     1381 acagagaaag aggcgcgcgc gctgtttgta gtgctggtac tgatttcgtt tttactggtg
     1441 ctgacgctga atacgatgac cattctgttg tcgattgccg cgctagcgct ggcgtgggtg
     1501 tacccgttta tgaagcggta tacccatcta ccgcaagtgg tgctgggcgc ggcgtttggc
     1561 tggtcgattc caatggcttt tgctgctgtg agtgagtcgg tgccattgag ttgctggtta
     1621 atgttcctcg ccaatattct ctgggcggtg gcttacgaca cgcagtatgc gatggttgac
     1681 cgtgatgatg atgtgaagat tggcattaaa tccaccgcaa tcctgttcgg ccaatacgat
     1741 aaattgatta ttggtatttt gcagattggc gtactggcac tgatggcgat catcggtgag
     1801 ttaaatggct taggctgggg atattactgg tcaattctgg tggctggcgc gctgtttgtt
     1861 tatcaacaaa aactgattgc caaccgcgag cgtgaagcct gctttaaagc atttatgaat
     1921 aataactatg ttggtctggt actgttttta gggctggcaa tgagttactg gcatttctga
     1981 tgatgtaaaa aagccggatg atcatccggc tttcttctgg gctgcctgat gcgttgcgct
     2041 tctcaggcct acacaacaga tcgcaattta ttgaatttgc agattatggt aagcctgata
     2101 aggcgtttgc gccgcatccg gcaattctct ctgattaccc ttcgccctgc gtcgcactct
     2161 caatcgtcaa acgcacgtct gatgtaatca actccgccag caactgataa accttcatcg
     2221 tttctgccgg ttcggcatcg ccgctatcgc tgatataccc ttcatcacgc agcgtcagca
     2281 ccagagaact gaacaccgct ttgtcgaaga actccggcgc gttgatgccg tgcagcacgg
     2341 agagacgttg cgcgacggtg cggctctctt tctccagcgt accgcggttg atcgacgggt
     2401 tggcgctcaa cagccagaag gtgatggcat aacgttgcag cgtttcgcgc gcgcctgcgg
     2461 cgagcagctg tagcgtgcga gaatgcgccg ggttgatatg caactcatca tcttgcaggg
     2521 taatcagccc ctgacgttgc atctcatttg ccagcgcatc aataacgtcc ggcaactcgt
     2581 cgcgatccca gcgcaggaac agctccgctt tcagcattgg gtaaagcaca ttgacgtgct
     2641 ccatcaatac gtcgcgggag atgtggcgat gctgggtgac aattgccgcc atcagcgaag
     2701 gcagcaccaa catatgcgca atgttgttgc gatagtaggt catcagcacc gcttgctcgc
     2761 gcggtagaat gatgatgtcg ccgatagtgt ctttctcgac ttcaaacttg ttcatttgca
     2821 gcgcgtgatc gataagctcg ctggcgctgg ctgaaggaac ggtagagtcc gtggagtaag
     2881 gtacgttgcg catcagatcc aggtagcagt tgagttgctc ggttaactgc tcgcgggtga
     2941 gtgagcgctg acgtgacgcc agtagcgcag tacagcacag gttcatggcg tttgccgcgc
     3001 ctgcgttgtt aatgcgtacc attagatcgg cagcaatatt attgaccgtc ggcgttaacc
     3061 aggccggacg caccgcttcg atgggatcga tagattcacg ccagtctggt acgtgctggt
     3121 taaggtaggt catcaacggc attggttcac cgaagttgac gtaaccctga ccgagattac
     3181 gcagcttgct taaaccgcgc agcatctgcg gcaggctctc tttctctttc gtcgcaccgc
     3241 gcagttcttt ggcgtaagta cccacttcca tgacgtgctc ataaccgata tagatcggaa
     3301 tcagcgtaat cggacgcgtg ccgccacgca gcatcgcctg aatggtcatc gacagcgtac
     3361 cggttttcgg atccagcaaa cgccccgtac gggaacgacc gccttccacg aagtactcga
     3421 cggaataacc acggctgaac agttcgccga gatactcacg gaaaacggtg gaatagagct
     3481 tattgccttt aaacgtacgg cgaataaaga acgcccccag acggcggaaa atcggcccgg
     3541 caggccagaa gttcaggttg atcccggcgg cgatatgcgg cggcaccagc ccctggtgat
     3601 acagcacgta agaaagcagc aggtagtcca tgtggctgcg gtggcaaggc acatatacca
     3661 gctcatgacc gtcgtgggcc agctggcgaa cgcgctcggc gttatggacg ttgatgccct
     3721 ggtaaaggcg gttccaggtg aagcccagaa tacggtcagt caggcggatc atctcgtaag
     3781 agaaattcgc cgcaatctct tccatcagcg caatcgcgtt ctgctgcgct ttttcatggg
     3841 agattttttt gctgcgcgct tcatcttcta ccgctttggc aatggcgcgg gaggcgagca
     3901 gcttattaaa cagatcctga cgagcaggca gacgtggacc cacggcagcc agacgttgac
     3961 gggcaaagtg catacgcgcc acgcgcgcca gtttctgcgc gatagtttta tccgtgccgt
     4021 gttcatccgc catacggcgc agcgaaactg acggcgagaa acgcacaaaa ctgtcgcgac
     4081 cgagccacag tacagcgaaa aatttctgta cgccgttaag catacgcagc ggcgggttca
     4141 cttcgccttt ttcacgccct ggcgcgcgac caaacatcac cgatactggc accatctgca
     4201 catccagatt tgggttgcta cggtgcaaat cgagatagtc gtggaacagc ttaatagact
     4261 cttctttcgg cgtgtaatag gtgaatacac gcggcccgcc gtgaatgaac acatagcgcg
     4321 gcagtagcgt gccgtcgatt tccagcggct ctaacgggtc aggcaggtca tgtgccagac
     4381 actgggcgcg caacgtcagc aagtctgctt tcgagttgta cggtaaaacg tacataattg
     4441 gacgagaggt atccagcccc agttccgggg caggatctgc cggaatagac ttgcttttta
     4501 ccaggatgct taatggtaaa ttcagtaatt tgtagtaaat tcgtggccag ccggacataa
     4561 acgatgtaaa gcctctggtt aataatgcaa atgcgcggca aggatagcag aaagtcatgg
     4621 gaaattctgt ggtatccgct catgtttcgc gcggcgttac gcaaacccga atcatcggat
     4681 ttaacggtac actgatattg acgctcataa tgtaaaaagg ttctttcaat ggccaataat
     4741 accactggat tcacccgaat aatcaaagct gctggctatt cctggaaagg tttacgcgct
     4801 gcatggatca acgaagcggc attccgtcag gaaggcgtag cggtattgtt ggcggtggtc
     4861 atcgcctgct ggctggatgt ggacgcaatt acccgcgtgc tgcttatcag ctccgtgatg
     4921 ctggtgatga ttgtggaaat cctcaatagc gccatcgaag cagtggttga ccgaattggc
     4981 tctgaatacc atgagctttc cggacgcgct aaagatatgg gatccgccgc ggtgctgatt
     5041 gcgattatcg tcgccgtgat tacctggtgc atcctgttat ggtcgcattt tggataaccc
     5101 ttccagattt cgataaatct ctggtttatt gtgcagttta tggttccaaa atcgcctttt
     5161 gctgtatata ctcacagcat aactgtatat acacccaggg ggcggaatga aagcgttaac
     5221 ggccaggcaa caagaggtgt ttgatctcat ccgtgatcac atcagccaga caggtatgcc
     5281 gccgacgcgt gcggaaatcg cgcagcgttt ggggttccgt tccccaaacg cggctgaaga
     5341 acatctgaag gcgctggcac gcaaaggcgt aattgaaatt gtttccggcg catcacgcgg
     5401 gattcgtctg ttgcaggaag aggaagaagg gttgccactg gtaggtcgtg tggctgccgg
     5461 tgaaccgctt ctggcgcaac agcatattga aggtcattat caggtcgatc cttccttgtt
     5521 caagccgaat gctgatttcc tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat
     5581 tatggatggc gacttgctgg cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt
     5641 tgtcgcacgt attgatgacg aagttaccgt taagcgcctg aaaaaacagg gcaataaagt
     5701 cgaactgttg ccagaaaata gcgagtttaa accaattgtc gttgaccttc gtcagcagag
     5761 cttcaccatt gaagggctgg cggttggcgt tattcgcaac ggcgactggc tgtaacatat
     5821 ctctgagacc gcgatgccgc ctggcgtcgc ggtttgtttt tcatctctct tcatcaggct
     5881 tgtctgcatg gcattcctca cttcatctga taaagcactc tggcatctcg ccttacccat
     5941 gattttctcc aatatcaccg ttccgttgct gggactggtc gatacggcgg taattggtca
     6001 tcttgatagc ccggtttatt tgggcggcgt ggcggttggt gcaacggcga ccagctttct
     6061 ctttatgctg ttgctgtttt tacgcatgag caccaccggg ctgactgcgc aggcttatgg
     6121 tgccaaaaat cctcaggcat tagcccgtgc gctggtgcaa ccgttgctgt tggcgttggg
     6181 ggctggggcg ttaattgcgc tgttgcgtac gccgattatc gatctggcgc tgcatattgt
     6241 tggcggtagt gaagcggtcc tggaacaggc gcggcgcttt cttgaaatcc gctggttaag
     6301 cgctccggcg tcgctggcga atctggtatt actcggttgg ttactcggcg tgcaatatgc
     6361 ccgtgcgcca gtaattttgt tagtggtcgg caatatcctc aacattgtgc tggatgtctg
     6421 gctggtgatg gggctgcata tgaacgtgca gggcgcggcg ctggcgacgg ttattgcgga
     6481 atatgcaaca ttgctgattg gtctgctaat ggtgcgtaaa atcctcaaac tacgcggaat
     6541 ttccggcgaa atgctgaaaa ctgcctggcg aggaaacttc cgtcgcttgc tggcgcttaa
     6601 ccgcgatatc atgctgcgtt cgctgttgtt gcaactctgt ttcggcgcga tcaccgtact
     6661 tggcgcgcga ctggggagtg acattatcgc tgttaacgcg gttctgatga cgctactcac
     6721 ctttaccgcc tatgcgctgg acggttttgc ctacgcggtt gaagcgcact ccggtcaggc
     6781 gtacggtgcg cgcgacggta gccagttgct ggatgtctgg cgggcagcgt gccgccagtc
     6841 ggggatcgta gcgttgctgt tttcggtggt ctatttgctg gcaggggaac acatcattgc
     6901 gttgctgacg tcgttaaccc agattcagca gctggccgac cgctatctta tctggcaggt
     6961 gattttgccg ttggttggcg tctggtgtta tctgctggac ggcatgttta taggcgcaac
     7021 gcgtgccgcc gaaatgcgta acagtatggc ggtggccgcc gcaggttttg cgctgacgct
     7081 ccttacgctg ccgtggctgg gcaatcatgg tttgtggctg gcgttaaccg tctttctggc
     7141 gttgcgcggg ctttctctgg cggctatctg gcggcgtcac tggcgcaatg gtacctggtt
     7201 tgccgcaacg tgacggttaa aaattctgaa taaataatcc taagccaaat tgctgactac
     7261 acttaatctc acgttcagaa gaaaagtgaa cgtactctca ttcacaacct aacgatgagg
     7321 tcttgattat gaataaagat gaagccggcg gtaactggaa acagtttaaa ggtaaagtga
     7381 aagagcaatg gggcaaactg accgatgatg atatgacgat cattgaaggt aaacgtgatc
     7441 aactggtcgg taaaatccag gaacgttatg gttatcagaa agatcaggca gaaaaagagg
     7501 tcgtggattg ggaaacccgc aatgaatatc gctggtaatt aatccctcct gcccgacgtg
     7561 tacaaggatg tacgccctct taacgcggtt tctttttcac ctgcacagag tgatcatgct
     7621 ggcactgttc aggatgacga cacgcttcca cttccacgca tgccgcacag agcccatgtg
     7681 cttcaatcac attatgccgc agggcaaacc ccatttttgc cgccagcgta tgcatgatgt
     7741 cttccacgcc ttctgcacat tcttctttca ccgcgccgca gcgatcgcaa ataaacatgg
     7801 ctgacgtatg ggtgggctgg tcgaacagat gacagagcac ataactgttg gtggattcca
     7861 ccttatgcac aaaaccttgc tcaagcagaa aatccagcgc gcgataaacc gttggcggct
     7921 tggcttgcgg ttcagcttcg cgcagcaaat caagcagatc gtaagcgctg atagcgccat
     7981 cctgcagact catcaggcgc aacacttcca ggcgctgtgg agtcaggcgc acattacgct
     8041 gcgcgcagat tttttcagcc tgcgctaata actcctgcgt tgtggtcttt tccatcgggc
     8101 acctcaaagt gggggttaag aatgtcctta ctttaccatg ttccaggaaa aacaccgaga
     8161 acccttattg ttgccgtaat gttgcttttc tgttttgtag gtaagatgtt gtgttgcctg
     8221 gcagtaccat tatcaacacg ataataatta ataattcatt tttaaataaa ttgcttcaag
     8281 ttaactcgct atttatttaa ccgtggctaa tcgtataccc gaatttcaga aaattatgag
     8341 cgatttttga tagtgaccgg ttccaaaaac acttataagg aggacacggt acgacaaaaa
     8401 aggtagatga atcat
//
LOCUS       DAZUCE010000212         8412 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZUCE010000212 DAZUCE010000000
VERSION     DAZUCE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.212"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(141..407)
                     /locus_tag="ACHY03_004123"
     CDS             complement(141..407)
                     /locus_tag="ACHY03_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFS3907298.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCSPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(404..1171)
                     /gene="yjiL"
                     /locus_tag="ACHY03_004124"
     CDS             complement(404..1171)
                     /gene="yjiL"
                     /locus_tag="ACHY03_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFS3907299.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            complement(1181..2332)
                     /locus_tag="ACHY03_004125"
     CDS             complement(1181..2332)
                     /locus_tag="ACHY03_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFS3907300.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDGASRALWKAEMLRLQKTVEKRFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARIRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2448..3728)
                     /locus_tag="ACHY03_004126"
     CDS             complement(2448..3728)
                     /locus_tag="ACHY03_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFS3907301.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3769..5001)
                     /gene="mdtM"
                     /locus_tag="ACHY03_004127"
     CDS             complement(3769..5001)
                     /gene="mdtM"
                     /locus_tag="ACHY03_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFS3907302.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPILFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            5468..6412
                     /locus_tag="ACHY03_004128"
     CDS             5468..6412
                     /locus_tag="ACHY03_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFS3907303.1"
                     /translation="MTNFTTSTPHDALFKSFLMHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYNAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEERFNEFISELTSRMPQHRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            complement(6654..8066)
                     /locus_tag="ACHY03_004129"
     CDS             complement(6654..8066)
                     /locus_tag="ACHY03_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFS3907304.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            8243..8407
                     /locus_tag="ACHY03_004130"
     CDS             8243..8407
                     /locus_tag="ACHY03_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFS3907305.1"
                     /translation="MEFYENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
BASE COUNT         2179 a         2233 c         2143 g         1857 t
ORIGIN      
        1 agacgtaaaa tagtaaggtg ctatcaaaaa tcgctcattt tgtggatggg aaatatttca
       61 tggcaggtga gatatttaat aaaacttcct tcatgcagga ttatgcgcag ccaattatcc
      121 ggttggtata tttaaagatc ctattgcctg gttgacgctt ctgcatcgta atgcaccacg
      181 cactgccagt cgctgccctg ctggcaatat atcgcttcgg tttgcccagg gatcacccat
      241 tgtatttcct cgccggggga acaggtcagc caaatgcata acccgcagcc gccgcgtaaa
      301 tcacggggaa tatcgctgac gcgaaaagtc atgcccgctg cctgcaacgc cttgcgggtt
      361 tgtatgacgc cgaccgtgga gtgaaataaa aataaaaact ctttcatcgg cgtgtcctca
      421 ctcgttgacc aattaccgcc gcgccaattg cgccagcaaa ttgcgcatca ggatgggtat
      481 ttaccggcat tcccaggtga gattccagca tccgggcaaa cgtctggcaa tgactaacgc
      541 caccagtaaa cagaatcggc gcttcacagg agagacgagc aatgaaattg gcactccgcc
      601 gcgccatcgc gttaatcact ccggcgagaa tcgcttctgg cgcgacgccc gctgagcgca
      661 ggctgatcac ttctgattcg gcaaacactg tgcacatact cgtgatggcg tgcggcgtga
      721 cattttcggt aatgctgtcg agctgctcga cgctggtgcc aagcgtgcgc gagatcacct
      781 ccaggaaacg cccggtgccc gccgcgcatt tgtcattcat caggaaatcg cacaggttac
      841 cgtcatcatc aagctgaatc actttgctgt cctgaccacc gatgtcgatt accgcgcgcg
      901 ttgctggcgc aagaaaccgt gcgcccagcc cgtgacagga gatttccgtc acttgtttat
      961 cggcaaaatc caccagttgc cgcccgtagc cggtgagcgt cagaaacggc gttgtctcta
     1021 acccttcgcg cagagtttcc caggcttcag taattgctgt tgccggacga aagggtgttg
     1081 gaacgaggaa acggcgcgta atcacgccgt ctgccagtaa gatccctttg gtggcggtag
     1141 aaccagaatc aatgccaatc gaatatgtca ctgctactcc ttacagcatc tcaataaagg
     1201 ccgcgacacg ggtactgagt tgcccaacat ccgaggtgga gtagtctgtt tcaatagcga
     1261 tataaggaat gttgtgctgc tgacgcacat gacgtttaat tgccagcgat tccaccgcgt
     1321 aggtatggca cgcctgcaaa atcacatcaa ctacgccatc gacctgatat tcttccacca
     1381 tctggctgag cattttcagg cgctgatcgt tcggcgaaac acaggagcag ccaatcgcca
     1441 gatatttatc cgccagcgcg tcgtagacat cgcccgtttc tgccacgcat tgctcggtcg
     1501 ctttcgcccc ggtgcagttt tcataaccga caacccagcc gccattctct tcaatcgcgc
     1561 gcaccacttt ctctgctgcg ccgccaatcg ggcagccggt gattaaaatg cgcggacgcg
     1621 ggtccagtcg ctggccttct tcccactgct gacgaatgcg ggcggtcatc gcgtccagtt
     1681 cattgatcaa cgcctcttta tcgaaccgga aggttgcgcc gtaaaccact ttcagaatgt
     1741 cgctgccgct aagcgccgga ggatttaact gcccaagatg ataaaaattg gccagtgcgc
     1801 gacgttcgcg gtttttcagc gcaatggcat cgcgcagagc atcttcgcta atctcgtgcc
     1861 caaaacgttt ttctaccgtt ttttgcaagc gcagcatctc ggctttccat aatgcacgcg
     1921 aggcaccgtc cttaacgctg ttcggcaact gcatcacatg aacgggctta aattccgcca
     1981 tgtattcata cattttcttt ttgccgtcgc aggtggtttc accgaccacc agatccgaaa
     2041 agtagaagta ggggcattta tcggttttgc caaagccgta gctgctttta atcagcgggc
     2101 agaggttgcg cggcagatct ttctccgctt cttcaatggt ttcatcagag gtggaacaga
     2161 gcgaaaccac aaccgcaccg gctgccatcg ggatctcttg cggcataaag gtgcagtaag
     2221 tgccaaccag cggaatgccg cgctccttga gatccatgac ggtgagaaag cctttctggc
     2281 gagcttcaga gaactgatcg aaaatggcgg gtagatcggt gacaagtgac atgattttcc
     2341 ttccccgtac cacgggagat aatgaaaaga ggccacatta ttaccattct tttagtatgg
     2401 tttatttgat ctctctcgcg gttagccact tagtttttca tggatttcta aaaattgccg
     2461 agggggaaca aggccgggag ctgcgacagc aaatataaaa ttagcccaat acaaccgcca
     2521 accagcgtac cgttgacacg gataaactgc agatctttgc cgatatttaa ctctatttgc
     2581 cgcgacatat cccgggcatc ccagcttttt accgtatcgc tgatgtggcg cgtcaggaat
     2641 gcggaaaact caggcgcgac gcggtgcgcg gcttgttcca gatgaccatt taacgacgcc
     2701 cgcaaggcat catcggcaat taacgtttcg ccaaaccatt gacccgcccg cgcgatacgt
     2761 tctttcacac gagaatcttc actgttgata tccgctttca gccattcccg taaatccccc
     2821 cacaattcac tgagatagcg gttaaaggct tcatcctctt tcagatagct ttttacggca
     2881 tcagctcgcg ctgccatttc aggatcgttt ttcaacttgt cgatcagggc aaaagtggcg
     2941 cgatcaaacg catggcggat ctgatgtgca cgatcgcggc tgatatcatc aagcaaagaa
     3001 ttcaccgcgt cagaaaccaa ctccgcgcta tgttcgccca gccattcggt ggggagaatt
     3061 ttggctttca gcggatgctc gctctccagc cagcgaacaa tttgctgggc gataaacttg
     3121 cgcgatttat cgcgctggag aagggcgatc aactgtgcga tcagcgtatc cagcagcacc
     3181 tgatgacgat cgtttttggt catactctcc agcatcaacg cactggtgcc ggaaagatcg
     3241 accttatcaa tcgcccgatg gaccgcgcgc ttaagcaggc gctgaatacg cgcatcatca
     3301 gttaattcga gaaaaccgct catgatctgc aacagatgct gaccaacgcg gcgggcgttt
     3361 tctggctgac taaaccagtt gccaatcaac aacgccggtt cgtggcgtcg aatcaatgcc
     3421 accagggatt gagtatcaag aaatttttcc tgcacgaact ggccgagatt ttcgccaatc
     3481 cggtctttat tacgcgggat aatcgctgta tgacgagaaa tgatcggaat cggcacgcgg
     3541 cgaaacagcg ccaccaccgc aaaccagtcc gccagcgcgc cgaccatcgc cgcttcggca
     3601 atcgccttca caccgctcac ccaaaaattg ggcggcaaaa acagcgtaac gacaaaggta
     3661 gcagcggcga taagcagtaa agagagcgcc aacattttgg cgcgtctgag ttcaatgagt
     3721 ttattcataa cgttaagaat agagcctgac ggcaagatcg cgcaaaaatc accgctcctc
     3781 cgccagctcg gcagcctgat gttggcgcac gcgattgagc aatcctgcca gggtgaaaac
     3841 gacgataacg cccgccacga cggctaacag atgaaacggc aagcgaccgc cgttaaacca
     3901 cagccaccgg ccgatttcga ccgagacaga catcaccatc aggatcacca tattcagcga
     3961 tgcggagacg gtccctttcg gtaagttatt ggaaaacagc gtaaagcgga ataagatcgg
     4021 gaaaatcaaa ccaatcccga aagcatacaa actggtgccc agcaccgacc acagccagac
     4081 gtgcggtgac agcagattgc cgataatcaa cagcgcgagg ccaaccagtt gaatgggtac
     4141 ggcacgccag ataaagcgcg gttcggtcgg atctttaacg aaacgcgcca cgatggcatt
     4201 cgcgacaatc accgcgccga ataccggaac ctgtgtccag gcgaactgcg aggtcgttaa
     4261 gccgcctgcg tcaatcagga tcaccggcga gacagccacc caactcatca tcgggatata
     4321 gcttaaagag atggttgctg cgccaaagag gaacagccga ttgcaaaaga cattgcggaa
     4381 atcgcgcaag acgcttttgg cgctaaatgg aaccgcgccg cgcttcactg tctctggcat
     4441 cgccagcagt aagccaacaa atgagataaa acccataacc gcaatgatgg caaaaaggac
     4501 tttccagtga acaaagtgca tcagagctgc gccggaaagc gggccaataa tcggcgcaat
     4561 cagtacgatg gaggtgataa tcgccatcaa cttgatccct tttgtctgtc cgaacgcctc
     4621 ctgcaccgtg acataaccaa cggtagcaat aaaacagata ctggtgccct gaattgcacg
     4681 cgcgataaga aactgtgtca tagacgttgt gaacattgtc gcagcgcagg caagggtaaa
     4741 aatgagcgcc ccggtaatca gcactggcct gcggccaatc ctgtcggaaa gtggccccag
     4801 cagccactgt aacgccatac cgccagcgag atagagactg acggcagcag gggccagact
     4861 gacatcggca ttaaaatcac ggaccacatt aatgatcccc ggctgaatca ggtccgtcga
     4921 caggtacgcg gcaaaatcat acaaaatcaa cgccatcggg aaaaacagcg tagcggcatg
     4981 gcgggcaaaa aaacgtggca taacaaacaa ctccttgtcc ggagaacccc aacggggaaa
     5041 acgaaattca ataccgtcgg taatgacgaa aggcaggcga gaaggacaaa tgtactgaat
     5101 ttcatgtaat caaccacact gcctgtgaac acacagacag agaaatacct tctgacttcg
     5161 ggtcagaatg tataacgatt acaaatagtc atcagcacat tacacctctt gaattgatgg
     5221 gttgcataga aaatatacac cttaagtgta attaaaattt gcggttaatc aaaagagcgc
     5281 ggtggaaggg ggaatatctg ccgggtacag gacatgtaaa acgcgagtca gtttccggaa
     5341 aaatgtatcg tttgcagacg aacaaaaata gcctggaaag cgcctcggga aacgagaaat
     5401 tgccgggtga gaatggtttt gttagtcgct aaagtcgggc catcttatca acaggtgacg
     5461 gatcgccatg acaaacttca cgaccagcac gccgcatgac gcattattta aatcttttct
     5521 catgcaccct gacaccgcgc gggattttat ggagatccac ttacccaaag atttacgtga
     5581 actgtgcgat ctcgacagct taaaactgga atccgccagc tttgtcgatg aaaaattgcg
     5641 ggcgctacat tccgatattc tgtggtcggt aaagacccgc gaaggcgatg gctatattta
     5701 tgtggtgatt gaacatcaga gccgcgagga tattcatatg gcctttcgcc tgatgcgcta
     5761 ttccatggcg gtgatgcagc gccatatcga gcatgataaa cgccggccgt taccgctggt
     5821 catcccgatg ctgttttatc acggtagccg tagtccttat ccctggtctt tgtgctggtt
     5881 ggacgaattt gccgacccga ctactgcacg gaagctttat aacgccgcgt tcccgctggt
     5941 ggatatcact gtcgtgccgg acgacgagat tgtgcagcac cgcagagtcg ccctgttgga
     6001 gttgatccaa aagcatattc gccagcgcga tctgatgggg cttatcgatc aactggtagt
     6061 attactggtt acagagtgtg ctaatgacag ccagataact gcgctgttaa attacatttt
     6121 actgactggc gatgaagagc gttttaatga gtttatcagt gaactaacca gtcgaatgcc
     6181 tcaacacagg gagcgaataa tgacgattgc agagcgaatt cataatgatg gatggctgtt
     6241 ggggatggaa aaggggaaag aagaagggga acaacgcctc cttagattgc ttttgcagaa
     6301 cggtgcggat cctgaatgga tacaacgata tactggactt tcggcagagc aaatgcaggc
     6361 attagagcaa cccttgcctg aaagcaagcg cgatccatgg atcgagtact aatcagagac
     6421 tgatgacaaa cgcaaagctg cctgatgtgc tacgcttatc agacctacat tacctctgca
     6481 atctattgaa tttgcgcgat gtgcaagccg gataaggcgt tcacgccgca tccggtatga
     6541 acaaagcaca ctttgcctaa gccatgctgg aacgtggcga tgcccctgca aagataaaac
     6601 gagtcacagg cgattcgtga gaggaaatca ggggcctcgc cactaattcg aattcattcc
     6661 attgcctgat acacggcctc gccaatttgc ttcagtgctt cgcgataggt ttcgctgagc
     6721 ggtaaagcgc agttgatacg cagacaatta cggtatttgc cggaagccga gaaaatcgag
     6781 cctgccgcca cctggatttt catgcggcac agctgccgcg caacgcaaac catatcgacc
     6841 tgttcaggca attctatcca cagtaaaaat ccgcctttcg ggcgcgtaat acagatttcg
     6901 cagggaaaat attcccgtat ccagcaggta taaagcgcca aattgcgctg atagatctgc
     6961 cgcatccgcc ggatatggcg atgatagtga ccttccagca caaacgttgc cgccgccatt
     7021 tgcgtggacg gcacattaaa gctgctgatg gcgtatttca tatgcagcag tttatcgtga
     7081 taacgccccg gtgcgaccca acccacgcgc aggcctggag caatactttt actgaacgag
     7141 ctgcacaaca gcactcgccc gtcgatatcc catgaatgaa tggtccgcgg gcgcggatac
     7201 tccgttgcca gttcgccgta gacatcatct tcaaaaatca caatatcatg acgctgagcg
     7261 agagagagaa cggcccgttt gcgcgcgtcc ggcataataa atcccagcgg attattacag
     7321 tttggcacca gaatgatgcc tttaatcggc cactgttcca gcgccagttc cagcgcttca
     7381 acgctgatgc cagtttctgg atcggttggg atttcaatca ctttcacgcc catgccgcgc
     7441 agcatctgca tcgaaccgta ataacaggga gattcgaccg cgacaatatc gcccggttta
     7501 cacaccgcca ttaacgccag cgacatcgag ttatggcagc cgctggtgat gatgatgtca
     7561 tcggcggtga ccaccgagcc gctgtcgagc atcaggcggg caatctgctc tcgcaatacc
     7621 cgctgaccgg ctaacaagtc ataaccgaga acggtttgca gattatgctg caccacccgg
     7681 cttagctcac gccacagcgg tttcaggctg ggcgtttcga catccggcgt gcttttgctt
     7741 aacggaacaa tggaactgtc gctatgcgcc tccagcatat ccagcacctg atcccactgg
     7801 gtaatttcca ccgggcgctg caccggacgc gtcattggtg gcactggcgg ctgggcttta
     7861 cgttgtgcga caaaataccc cgaacgcggc tgcggagtga tgagcttcat cgtttccagc
     7921 gtctgatacg cctgctgcac ggtgctgatg ctgacgccgt gctcctgact taagctgcgc
     7981 accgacggca atttctcccc gtgacgatac agcccttgct caatccgctc ggcaagtaga
     8041 gtcgccagat gttgataacg cgtcatgctg tattccttat atggaccata cagagagaga
     8101 aaccggtaca gatgaggcta aaaaaccgga tgcagatact gttttaacct gtctgtatgt
     8161 taaataaaac taatatttgc atctgtattg tgaactgcca gaaggcagat gatgaagcct
     8221 ctgaaagatg aggaggcagc agatggaatt ttacgaaaac aaagccaaaa cgccgtttat
     8281 cagacttgta caactctggc aggcggtgag gcgttggcgg cggcaaatgc aggcccgacg
     8341 ggtgttacag cagatgagtg atgagcggtt gagggatatt gggttacgca gggaggatgt
     8401 ggagtgaggg gg
//
LOCUS       DAZUCE010000213         8375 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZUCE010000213 DAZUCE010000000
VERSION     DAZUCE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.213"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..625)
                     /gene="upp"
                     /locus_tag="ACHY03_004131"
     CDS             complement(<1..625)
                     /gene="upp"
                     /locus_tag="ACHY03_004131"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFS3907306.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            950..1987
                     /gene="purM"
                     /locus_tag="ACHY03_004132"
     CDS             950..1987
                     /gene="purM"
                     /locus_tag="ACHY03_004132"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFS3907307.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            1987..2625
                     /gene="purN"
                     /locus_tag="ACHY03_004133"
     CDS             1987..2625
                     /gene="purN"
                     /locus_tag="ACHY03_004133"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFS3907308.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2797..4863
                     /gene="ppk1"
                     /locus_tag="ACHY03_004134"
     CDS             2797..4863
                     /gene="ppk1"
                     /locus_tag="ACHY03_004134"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFS3907309.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4868..6409
                     /gene="ppx"
                     /locus_tag="ACHY03_004135"
     CDS             4868..6409
                     /gene="ppx"
                     /locus_tag="ACHY03_004135"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFS3907310.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMELGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6448..>8375)
                     /gene="pdeF"
                     /locus_tag="ACHY03_004136"
     CDS             complement(6448..>8375)
                     /gene="pdeF"
                     /locus_tag="ACHY03_004136"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFS3907311.1"
                     /translation="TPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLA
                     SRENLVGVMPFNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVD
                     VKVTKKEFALWLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLI
                     SLLWAVVLMISIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVR
                     RIQRLAYVDPVVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQK
                     LSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERIAALDSHLKQFRFFWDGMPMQPQ
                     IGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMN
                     RLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSS
                     IDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLI
                     FEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSF
                     IRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKP
                     QPLIDTLNEIEPIRESA"
BASE COUNT         2121 a         2073 c         2099 g         2082 t
ORIGIN      
        1 atttcgtacc aaagattttg tcaccggcat cgccgaggcc cggaataatg tatccgtgct
       61 cgttcagtcc ctgatcaatc gatgcggtat acagttcgac gtccgggtgc gctttttcca
      121 gcgcagcgat accttctggc gcagctacca gcaccagaac tttgatgctg ctgcagcccg
      181 cttttttcag caggtcgatg gtcgcgataa cggaaccacc ggttgccagc attgggtcaa
      241 cgatcagcgc catacgctca tcgatgttag aaaccagttt ctggaagtac ggtaccggct
      301 ccagcgtttc ttcattacgg tacataccga caacgctgat gcgcgcgctc ggaacgtttt
      361 ccagcacacc gtccatcatc cccagacccg cacgcagaat tggcacaacg gtaattttct
      421 tacctttgat ctggtcgatt tctaccgggc cgttccagcc ttcgatagtt actttttccg
      481 tttcgaggtc ggcggtcgct tcgtaagtca gcaggctacc cacttcggaa gcgagttcgc
      541 gaaagcgctt ggtgctgata tcttgctcac gcatcagtcc cagcttgtgt ttgacgagtg
      601 ggtgtttgac ttccacgatc ttcatactct ttctcctttg aggggcagcc acaaaaaaaa
      661 tcgacggatt atacctcctt tcttcaaggc ggcaatatta ttttcgttga ctttagtcaa
      721 aatgataacg gtttgagata aagttatttt atattcaaat ggttatgaaa gaagattatt
      781 ccatccgaaa actaaccttt gccctggcac aagtcttctt tcgccgcgcg cctggggaaa
      841 agacgtgcaa aaaggttgtg taaagcagtc tcgcaaacgt ttgctttccc tgttagaatt
      901 gcgccgaatt ttatttttct accgcaagta acgcgtgggg acccaagcag tgaccgataa
      961 aacctctctt agctacaaag atgccggtgt tgatattgac gcgggtaatg ctctggttgg
     1021 aagaatcaaa ggcgtagtga agaaaacgcg tcgtccggaa gtgatgggcg gtctgggcgg
     1081 cttcggtgcg ctgtgtgcat tgccgcaaaa atatcgtgaa cccgtgctgg tttccggcac
     1141 tgacggcgta ggtaccaagc tgcgtctggc aatggactta aaacgtcacg acaccattgg
     1201 tattgatctg gtcgccatgt gcgttaatga cctggtggtg caaggtgcag aaccgctgtt
     1261 tttcctcgac tattacgcaa ccggaaaact ggatgttgat accgcttcag cggtgatcag
     1321 cggcatcgcg gaaggttgtc tgcaatcggg ctgttctctg gtgggtggcg aaacggcaga
     1381 aatgccgggg atgtatcacg gtgaagatta cgatgtcgcg ggtttctgcg tgggcgtggt
     1441 agaaaaatca gaaatcatcg acggctctaa agtcagcgac ggcgatgtgc tgattgcact
     1501 cggttccagc ggtccgcact cgaacggcta ttcgctggtg cgcaaaattc ttgaagtcag
     1561 cggttgtgat ccgcaaacca ccgaacttga tggtaagcca ttagccgatc atctgctggc
     1621 accgacccgc atttacgtga agtcagtgct ggagttgatt gaaaaggtcg atgtgcatgc
     1681 cattgcgcac ctgaccggcg gcggcttctg ggaaaacatt ccgcgcgtat tgccagataa
     1741 tacccaggca gtgattgatg aatcttcctg gcagtggccg gaagtgttca actggctgca
     1801 aacggctggt aacgttgagc gccatgaaat gtatcgcacc ttcaactgcg gcgtcgggat
     1861 gattatcgcc ctgcctgctc cggaagtgga caaagccctc gccctgctca atgccaacgg
     1921 tgaaaacgcg tggaaaatcg gtatcatcaa agcctctgat tccgaacaac gcgtggttat
     1981 cgaataatga atattgtggt gcttatttcc ggcaacggaa gtaatttgca ggcaattatt
     2041 gacgcctgta aaaccaacaa aattaaaggc accgtacggg cagttttcag caataaggcc
     2101 gacgcgttcg gccttgaacg cgcccgccag gcgggtattg caacgcatac gctcatcgcc
     2161 agcgcgtttg acagtcgtga agcctatgac cgggagttga ttcatgaaat cgacatgtac
     2221 gcacccgatg tggtcgtgct ggctggtttt atgcgcattc tcagcccggc gtttgtctcc
     2281 cactatgccg ggcgtttgct gaacattcac ccttctctgc tgccgaaata tcccggatta
     2341 cacacccatc gtcaggcgct ggaaaatggc gatgaagagc acggtacatc ggtgcatttc
     2401 gtcaccgatg aactggacgg tggcccggtt attttacagg cgaaagtccc ggtatttgct
     2461 ggtgatacgg aagatgacgt caccgcccgc gtgcaaaccc aggaacacgc catttatcca
     2521 ctggtgatta gctggtttgc cgatggtcgt ctgaaaatgc acgaaaacgc cgcgtggctg
     2581 gatggtcaac gtctgccgcc gcagggctac gctgccgacg agtaatgccc ccgtagttaa
     2641 agcgccagct ctgccgctgg cgtttttcaa ttcacctgta aatcgcaagc tccagcagtt
     2701 tttttccccc ttttctggca tagttggaca tctgccaata ttgctcgcca taatatcctg
     2761 gcagtgtccc gtgaataaaa cggagtaaaa gtggtaatgg gtcaggaaaa gctatacatc
     2821 gaaaaagagc tcagttggtt atcgttcaat gaacgcgtgc ttcaggaagc ggcggacaaa
     2881 tctaacccgc tgattgaaag gatgcgtttc ctggggatct attccaataa ccttgatgag
     2941 ttctataaag tccgcttcgc tgaactgaag cgacgcatca ttattagcga agaacaaggc
     3001 tccaactctc attcccgcca tttactgggc aaaattcagt cccgggtgct gaaagccgat
     3061 caggaattcg acggcctcta caacgagcta ttgctggaga tggcgcgcaa ccagatcttc
     3121 ctgattaatg aacgccagct ctccgtcaat caacaaaact ggctgcgtca ttattttaag
     3181 cagtatctgc gtcagcacat tacgccgatt ttaatcaatc ctgacactga cttagtgcag
     3241 ttcctgaaag atgattacac ctatttggcg gtggaaatta tccgtggcga taccatccgt
     3301 tacgcgctgc tggagatccc atcagataaa gtgccacgct ttgtgaattt accgccagaa
     3361 gcgccgcgtc gacgcaaacc gatgattctt ctggataaca ttctgcgtta ctgcctcgat
     3421 gatattttca aaggcttctt tgattatgac gcgctgaatg cctattcaat gaagatgacc
     3481 cgcgatgccg aatacgattt agtgcatgag atggaagcca gcctgatgga gttgatgtct
     3541 tccagtctca agcagcgttt aactgctgag ccggtgcgtt ttgtttatca gcgcgatatg
     3601 cccaatgcac tggttgaagt tttacgcgaa aaactaacta tttcccgcta cgactccatc
     3661 gtccccggcg gtcgttatca taattttaaa gactttatta atttccccaa tgtcggcaaa
     3721 gccaatctgg tgaacaaacc actgccgcgt ttacgccata tttggtttga taaagcccag
     3781 ttccgcaatg gttttgatgc cattcgcgaa cgcgatgtgt tgctctatta tccttatcac
     3841 acctttgagc atgtgctgga actgctgcgt caggcttcgt tcgacccgag cgtactggcg
     3901 attaaaatta acatttaccg cgtggcgaaa gattcacgca tcatcgactc gatgatccac
     3961 gccgcacaca acggtaagaa agtcaccgtg gtggttgagt tacaggcgcg tttcgacgaa
     4021 gaagcaaaca ttcactgggc gaagcgcctg accgaagcag gcgtgcacgt tatcttctct
     4081 gcgccggggc tgaaaattca cgccaaactg ttcctgattt cacgtaaaga aaacggtgaa
     4141 gtggtgcgtt acgcacacat cgggaccgga aactttaacg aaaaaaccgc gcgtctttat
     4201 actgactatt cgttgctgac cgccgatgcg cgcatcacta acgaagtacg gcgggtgttc
     4261 aactttattg aaaacccata ccgtccggtg acatttgatt atttaatggt gtcaccgcaa
     4321 aactctcgcc gtctgttata tgaaatggtg gaccgcgaga tcgccaacgc gcagcaaggg
     4381 ttgcccagtg gtatcaccct gaagctaaat aaccttgtcg ataaaggcct ggttgatcgt
     4441 ctgtatgcgg cctccagctc cggcgtaccg gttaatctgc tggttcgcgg aatgtgttcg
     4501 ctgatcccca atctggaagg cattagcgac aacattcgtg ccatcagtat tgttgaccgt
     4561 taccttgaac atgaccgggt ttatattttt gaaaatggcg gcgataaaaa ggtctacctt
     4621 tcttctgccg actggatgac gcgcaatatt gattatcgta ttgaagtggc gacgccgctg
     4681 ctcgatccgc gcctgaagca gcgggtgctg gacatcatcg acatattgtt cagcgatacg
     4741 gtcaaagcac gttatatcga taaagaactc agtaatcgct acgttccccg cggcaatcgc
     4801 cgcaaagtac gggcgcagtt ggcgatttat gactacatca aatcactcga acaacctgaa
     4861 taaccctatg ccaatacacg ataaatcccc tcgtccgcag gagtttgctg cggtcgatct
     4921 tggttcaaac agttttcaca tggtcatagc ccgtgtggta gatggtgcca tgcagattat
     4981 tggccgcctg aaacagcggg tgcatctggc ggacggcctg gggccagata atatgttgag
     5041 tgaagaggca atgacgcgcg gtttgaactg tctgtcgctg tttgccgaac ggctacaagg
     5101 gttttctcct gccagcgtct gtatagttgg tacccatacg ctgcgtcagg cgctgaacgc
     5161 cactgacttt ctgaaacgtg cggaaaaggt cattccctac ccgattgaaa ttatttccgg
     5221 taatgaagaa gcccgtctga tttttatggg cgtggaacat acccaaccgg aaaaaggtcg
     5281 caaactggtt attgatattg gcggcggttc tacagaactg gtgattggtg aaaatttcga
     5341 accgattctc gttgaaagcc gccggatggg gtgtgtcagc tttgcccagc tttatttccc
     5401 tggcggagtc atcaataaag agaattttca gcgtgctcgc atggcggcag cacaaaaact
     5461 ggaaacttta acctggcaat ttcgaattca aggctggaac gtggcaatgg gcgcttccgg
     5521 taccataaaa gccgcccatg aagtgttaat ggaattgggt gagaaagacg ggataattac
     5581 cccggaacgt ctggaaaaac tggtaaaaga agttttacgg caccgtaatt tcgcatcgct
     5641 gagtttacca ggtctttccg aagagcggaa aacagtcttc gttccgggac tggcgatttt
     5701 atgcggtgtg tttgatgctt tagccatccg tgaactgcgc ctttctgacg gggcgttacg
     5761 cgaaggcgta ctgtatgaaa tggaaggacg tttccgtcat caggatgtgc gtagtcgcac
     5821 cgccagcagc ctcgccaacc agtatcacat cgacagcgaa caggcccgac gagtgctgga
     5881 taccactatg caaatgtacg aacagtggcg ggaacagcaa ccgaagctgg cgcatccgca
     5941 actggaggcg ctactgcgat gggccgccat gctgcatgag gtcgggttga atatcaacca
     6001 cagcggtttg catcgccact ccgcttatat tctgcaaaac agtgacttgc cgggttttaa
     6061 tcaggaacag cagctgatga tggcgacact ggtgcgctat caccgtaaag cgattaaact
     6121 cgacgatctg ccgcgcttta ccttgtttaa gaagaaacag ttcctgccac tgattcagct
     6181 attgcgcctt ggcgtattac tcaacaatca acgtcaggca accaccacac cgccaacatt
     6241 gacactgatt actgatgaca gtcactggac actgcgtttc ccgcatgact ggtttagtca
     6301 gaatgcgctg gtactgcttg atctggaaaa ggagcaagaa tactgggaag gcgtggctgg
     6361 ctggcggttg aaaattgaag aagaaagtac acctgaaatc gccgcttaat gctttgcggg
     6421 ccgacgagaa atgtcggccc gcattattca ggcactttcg cgaatgggtt cgatttcatt
     6481 cagcgtatca attaacggtt gcggcttacc aataagataa ccctgcatat aatcgatccc
     6541 caaagagagc accgcctcgc ggatctcttc gttttcaacg tactctgcca ctacccgcat
     6601 tttcttcatt cgcgccaggt ggcaaatcga tgccactatc tgataatcca gactatttga
     6661 cacaatattg cggataaaac tgccgtcaat tttaagcaga tcggcattca catttttaag
     6721 ccgcgcatag ctggcgtagc cggtgccgaa atcatcaatc gcaatctggc agcctaattc
     6781 ctgaagatgc tgcaaggtga tttgcgcctg cttaacattg gtcagagcat tactttcggt
     6841 gacttcaaaa ataagttgcc acgcttcaat ctgatattta tccagcaact gactgacttc
     6901 aacaggaaaa cgagcctgac ataccgaggt tggagacaga ttaatagcaa aacggtgagc
     6961 gggcatcttc gctctgtttt cagccataaa ttgcagcgta tgctcaatga cccacatgtc
     7021 gatactcgac gataaaccaa attcgtgcgc gaccggcaag aagctatcgg ggccgatcag
     7081 ttcatcattc tcacctttca tgcgcagaag aatttcatgg taaacatcac cacgcatacc
     7141 ggtaatcggc tgggccatca ggaaaaaatg gttgtgttcc agcgcctgct gtagtcgatt
     7201 catcatcgcg actttatctt tcaattcgcg ttgcaaatac attgccccgc gacgctgcat
     7261 attttccggg gcgttggtca cgatggaaag ttcggcgacc gtatttagct ctcccagcag
     7321 caggtagata tgattcactg gcgagcgcac atagcagtaa ctgacgccaa tctgcggttg
     7381 catcggcatg ccatcccaaa agaaacgaaa ttgcttgaga tggctatcca gtgcggcaat
     7441 gcgctcctgg tgcgattctg tattcagtcg cagcgcgaga tcgttacccg aaagctgata
     7501 aacatcttca cccggttcca gcaatggtga cagccagtga gaaagttttt gcttgtattg
     7561 aatccgcagc atgatgccat agtttttaac cagcatttcc atgccaggga tgcgtaaata
     7621 acaaagcgca gaccaggggg catcacgcaa cgcgcgattc agggcgcgaa catttggcag
     7681 atgaaccacc gggtccacat acgcaagccg ttgaatgcgt ctgactaccg ctctctgacg
     7741 ggttgccagt acagccatat aattgacaat aaaagagaat accagataac ttgaggaggt
     7801 tatggtcagc tgcgtggtat agccaggata aatgggaatg taattttgat agctgtggat
     7861 gctgatcatc aacacgaccg cccagagcag tgaaatcagc ttataaccat agcgcatcgc
     7921 tccccacatc atcaggggca gcaataatga caaggtataa ttggtgctaa aaattgtgct
     7981 tttttcattt aacggcatgc ataacagcaa cagtaaagca cctaatgcca gtagccagag
     8041 tgcgaactct tttttggtga ctttgacatc aacctgctgt tttaattgcg aataataact
     8101 acgtaaataa aacggatttc gaactacccg aatgatgaag tagcacagcg ggacaccaat
     8161 cagattgccc accagtaagg cctgataatt gattaaggtc ccgaggttaa aaggcatgac
     8221 gccgaccaga ttttctctgc tcgccagtaa tcctacaaac gcagcaaact ggaaaagtat
     8281 cagaaacagc gttgcaggaa acacaatctg ccagaatata cgctgggaaa ttaaacgggt
     8341 atcaccatgt gaaacgttgt tacgccgggg agtaa
//
LOCUS       DAZUCE010000214         8356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZUCE010000214 DAZUCE010000000
VERSION     DAZUCE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.214"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(194..1084)
                     /gene="malG"
                     /locus_tag="ACHY03_004137"
     CDS             complement(194..1084)
                     /gene="malG"
                     /locus_tag="ACHY03_004137"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFS3907312.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1099..2643)
                     /gene="malF"
                     /locus_tag="ACHY03_004138"
     CDS             complement(1099..2643)
                     /gene="malF"
                     /locus_tag="ACHY03_004138"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFS3907313.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2797..3987)
                     /gene="malE"
                     /locus_tag="ACHY03_004139"
     CDS             complement(2797..3987)
                     /gene="malE"
                     /locus_tag="ACHY03_004139"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFS3907314.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4352..5467
                     /gene="malK"
                     /locus_tag="ACHY03_004140"
     CDS             4352..5467
                     /gene="malK"
                     /locus_tag="ACHY03_004140"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFS3907315.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5539..6879
                     /gene="lamB"
                     /locus_tag="ACHY03_004141"
     CDS             5539..6879
                     /gene="lamB"
                     /locus_tag="ACHY03_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFS3907316.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEVNPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDDWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7122..8042
                     /gene="malM"
                     /locus_tag="ACHY03_004142"
     CDS             7122..8042
                     /gene="malM"
                     /locus_tag="ACHY03_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFS3907317.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            8271..>8356
                     /locus_tag="ACHY03_004143"
     CDS             8271..>8356
                     /locus_tag="ACHY03_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907318.1"
                     /translation="MLGHISKFDGNNSLIKHGVVQGNNIVDF"
BASE COUNT         2061 a         2183 c         2163 g         1949 t
ORIGIN      
        1 aaagagaatt atcgtaagag ggaaaacaaa taacgtaaaa ataataagtt ctatgatgga
       61 aatattaacc ggcgaacgat tcagattgca gacgaaagaa aaaaaggcgc tccgtggagc
      121 gccgaataac agtcacaagt tgggataacg taagttgagg gtgcagcggc ataacattgg
      181 cagaacaaca tctttaacct ttcacaccac ctgccgtcag gccgttgacc agccagcgtt
      241 gagccagcaa gaagacgatg gtgatcggta atgcagacat cacggcagcg gcggcaaagt
      301 caccccacag gtagttttgc ggattgaggt attgctgcat ccccacggcc agggtgtagc
      361 tgtttacgtc acgcagtaac agcgacgcga ccggaacttc agtaatggca gcgataaacg
      421 acaggatgaa taccaccgcc agaatcggta ctgacagcgg caacaggaca aggcggaagg
      481 cctgccacgg tgtcgcacca tccagcgccg ccgcttcttc cagcgaactg tcgatggttt
      541 cgaaatagcc tttgatggtc cagacgtgca gcgcaatccc acccagatac gcgaaaatta
      601 cgccgccgtg agtattcagg ccaatgaatg gaatgtactc acccagacga tcaaacaacg
      661 catacaacgc gaccagtgaa agtactgccg ggaacatctg gaaaatcagc attcctttca
      721 gcagcgtcgc tttgcctgga aagcgcatac gggcgaaagc gtaggcgcag gtggtggaga
      781 gcgccacaat gccaatcgcg gaaatcccgg cgacctttac cgagttccac agccacagca
      841 gtaccgggaa tggcggtggc gtaatgcgac catcagcctg ttccacgcta aaacctaacg
      901 ccagtttcca gtgatcccag gagatttgct ccgggatcag gctaccggtc gcgaagtttc
      961 cctgacgcag cgagatagcg acgaccatca gcagcgggaa cataatcgcc gcgataaaaa
     1021 gtagcagtag caggtgagta ataaacaaac gtgctttttg cgatttcggt tggaccattg
     1081 ccatttttgt tatctccctt aatcaaactt cattcgcgtg gctttcaggt tcactatcgc
     1141 cagcgcaccc accagcagga agatcagcgt ggcaattgct gctgccagac cgaagtcctg
     1201 acccccgccg ccttcaaaag cgatgcggta ggtgtagtta acaagcaggt cggtataacc
     1261 ggctggcgtg gtcgtgccaa gacgatccgg gccgccgttg gttaacagtt gaatcagcac
     1321 gaagttgtta aagttaaagg cgaagctggc gatcatcagc ggcgtcagcg gtttaatcag
     1381 cagcggcagc gtaatcttaa agaagttctg gaacggacct gcaccatcca ttgctgaggc
     1441 ttcatacaaa tcgtccggaa tcgctttcag caagcccatg cagaggatca tcatgtacgg
     1501 atagcccagc caggtattga cgataatcaa catcgttcgg gcggtggtcg gatcgctgaa
     1561 ccaggcgggc ttcacgccaa acagcgcgct caacatcatg ttgatctcac cgaagctctg
     1621 gttaaacaac cctttgaaaa tcaagattga aatgaacgat ggcaccgcgt agggcagaat
     1681 cagcaggacg cgatagaccg ctttgccgcg caacgcttcc cactgcacca gacacgccag
     1741 aaccatgccg actgccaccg ttaaaaagac agtgatcagc gagaacacca cggtccagac
     1801 gaaaatggcg aggaacggtt tctgaatgcc ttcgtcggtg aagacgcgag taaagttttt
     1861 ccagccggtg gtcacggtgt aaccggggct tagcttttca tcaccccagt tgccgtcggc
     1921 ggtaatggac tggtaaaagc caatttggtt attcggacga tatttcacgc cgctctgatt
     1981 attcgtcaac gtgccgtcac cgtcgagtgt gtagagcggc tgcgtgccag aaaactggcg
     2041 cagggagctc atcatcactt tgttgccatc cggcagaatg gcggtaatgt cactcagcgc
     2101 ctgacgattc tgggtaatca cgcgtagatt cgcgcgttcg ccttcgggct gggtggtcgt
     2161 ttctttcagt tgcagttttt gctcgccgcc aaatttaaaa gcgtcggaga ggtaattttt
     2221 gccggtttcg ccgtcgctga gggctagttg ccactcatcg cccgccgggt aaagaccaaa
     2281 attataggtt ttgcctgctt gccaggagcg atctagcaac acttcctgcg cacgttcaaa
     2341 agtcagctgg ttagtgctgc tgtagttggt gaaggcaatg gcgatggtgc agaccagagg
     2401 gaagaggacg aataatccca ttccagccat tcccgggtaa acatagcgcc aggcgtaggc
     2461 tttacgattg gcgaaaatat acagccccgc tgaactcaat atcagcgtgg taatggcgaa
     2521 caggtattcc ccttgtgcgt acattaaaac aacaaggtaa cccaccagca ggccgagcag
     2581 acctaacact gaccatttca gcgcgtcgct ttgccaccaa tgtttctttt taatgacatc
     2641 catggggttc ttcctcattc caggacggat aaggctttca cgccttatcc gacaacaact
     2701 gcctgatgcg acgctgacgc gtcttatcag gcctacatac gtttcggttt tgtaggccgg
     2761 acaaggcgtt cacgccgcat ccggcatttc acagcattac ttggtgatac gagtctgcgc
     2821 gtctttcagg gcttcatcga cagtctgacg accgctggcg gcgttgatca ccgcagtacg
     2881 cacggcatac cagaaagcgg acatctgcgg gatgttcggc atgatttcac ctttctgggc
     2941 gttttccatg gtggcggcaa tacgtggatc tttcgccaac tcttcctcgt aagacttcag
     3001 cgctacggca cccagcggtt tgtctttatt aaccgcttcc agaccttcgt cagtcagcag
     3061 atagttttcg aggaactctt tcgccagctc tttgttcgga ctggcggcgt taataccggc
     3121 gctcagcacg ccaacgaacg gtttagatgg ttgacctttg aaggtcggca gtaccgttac
     3181 accataattc actttgctgg tgtcgatgtt ggaccatgcc cacgggccgt tgatggtcat
     3241 cgctgtttcg cctttattaa aggcagcttc tgcgatggag taatcggtgt ctgcattcat
     3301 gtgtttgttt ttaatcaggt caaccaggaa ggtcagaccc gctttcgcgc cagcgttatc
     3361 cacgcccacg tctttaatgt cgtacttgcc gttttcatac ttgaacgcat aacccccgtc
     3421 agcagcaatc agcggccagg tgaagtacgg ttcttgcagg ttgaacatca gcgcgctctt
     3481 acctttcgct ttcagttctt tatccagcgc cgggatctct tcccaggttt ttggcgggtt
     3541 cggcagcaga tctttgttat aaatcagcga taacgcttca acagcgatcg ggtaagcaat
     3601 cagcttgccg ttgtaacgta cggcatccca ggtaaacgga tacagcttgt cctggaacgc
     3661 tttgtccggg gtgatttcag ccaacaggcc agattgagcg tagccaccaa aacggtcgtg
     3721 cgcccagaag ataatgtcag ggccatcgcc agttgccgca acctgaggga atttctcttc
     3781 cagtttatcc ggatgctcaa cggtgacttt aattccggta tctttctcga atttcttacc
     3841 gacttcagcg agaccgttat agcctttatc gccgttaatc cagattacca gtttaccttc
     3901 ttcgattttg gcgagagccg aggcggaaaa catcatcgtc gttaatgcgg ataatgcgag
     3961 gatgcgtgca cctgttttta ttttcataat ctatggtcct tgttggtgaa gtgctcgtga
     4021 aaacacctaa acggactcta gtttctttat acggcaacct ctttccatcc tccttgcccc
     4081 tacgccccac cgttgctttg tgtgatctct gttacagaat tggcagtaat gtggagatgc
     4141 gcacataaaa tcgccacgat ttttgcaagc aacatcacga aattccttac atgacctcgg
     4201 tttagttcac agaagccgcg ttctcatcct cccgcctcct cccccataaa aaagccaggg
     4261 ggtggaggat ttaagccatc tcctgatgac gcatagtcag cccatcatga atgttgctgt
     4321 cgatgacagg ttgttacaaa gggagaaggg catggcgagc gtacagctgc aaaatgtaac
     4381 gaaagcctgg ggcgaggtcg tggtatcgaa agatatcaat ctcgatatcc atgaaggtga
     4441 attcgtggtg tttgtcggac cgtctggctg cggtaaatcg actttactgc gcatgattgc
     4501 cgggcttgag acgatcacca gcggcgacct gttcatcggt gagaaacgga tgaatgacac
     4561 tccgccagca gaacgcggcg ttggtatggt gtttcagtct tacgcgctct atccccacct
     4621 gtcagtagca gaaaacatgt catttggcct gaaactggca ggcgcaaaaa aagaggtgat
     4681 taaccaacgc gtcaaccagg tggcggaagt gctacaactg gcgcatttgc tggatcgcaa
     4741 accgaaagcg ctctccggtg gtcagcgtca gcgcgtggcg attggccgta cgctggtggc
     4801 cgagccaagc gtatttttgc tcgatgaacc gctctccaac ctcgatgctg cactgcgtgt
     4861 gcaaatgcgt atcgaaatct cccgtctgca taaacgcctg ggccgcacaa tgatttacgt
     4921 cacccacgat caggtcgaag cgatgacgct ggccgacaaa atcgtggtac tggacgccgg
     4981 tcgcgtggcg caggtaggga aaccgctgga gctgtaccac tatccggcag accgttttgt
     5041 cgccggattt atcggttcgc caaagatgaa cttcctgccg gtaaaagtga ccgccaccgc
     5101 aatcgatcaa gtgcaggtgg agctgccgat gccaaatcgt cagcaagtct ggctacctgt
     5161 tgaaagccgt gatgtccagg ttggagccaa tatgtcgctg ggtattcgcc cggaacatct
     5221 actgccgagt gatatcgctg acgtcatcct tgagggtgaa gttcaggtcg tcgagcaact
     5281 cggcaacgaa acccaaatcc atatccagat cccttccatt cgtcaaaacc tggtgtaccg
     5341 ccagaacgac gtggtgttgg tagaagaagg tgccacattc gctatcggcc tgccgccaga
     5401 gcgttgccat ctgttccgtg aggatggcac tgcatgtcgt cgactgcata aggagccggg
     5461 cgtttaagca ccccacaaaa cacacaaagc ctgtcacagg tgatgtgaaa aaagaaaagc
     5521 aatgactcag gagatagaat gatgattact ctgcgcaaac ttcctctggc ggttgccgtc
     5581 gcagcgggcg taatgtctgc tcaggcaatg gctgttgatt tccacggcta tgcacgttcc
     5641 ggtattggct ggacaggtag cggcggtgaa caacagtgtt tccagactac cggtgctcaa
     5701 agtaaatacc gtcttggcaa cgaatgtgaa acttatgctg aattaaaatt gggtcaggaa
     5761 gtgtggaaag agggcgataa gagcttctat ttcgacacta acgtggccta ttccgtcgcg
     5821 caacagaatg actgggaagc taccgacccg gccttccgtg aagcaaacgt gcagggtaaa
     5881 aacctgatcg aatggctgcc aggctccacc atctgggcag gtaagcgctt ctaccaacgt
     5941 catgacgttc atatgatcga cttctactac tgggatattt ctggtcctgg tgccggtctg
     6001 gaaaacatcg atgttggctt cggtaaactc tctctggcag caacccgctc ctctgaagca
     6061 ggtggttctt cctctttcgc cagtaacaat atttatgact ataccaacga aaccgcgaac
     6121 gacgttttcg atgtgcgttt agcgcagatg gaagtcaacc cgggcggcac attagaactg
     6181 ggtgtcgact acggtcgtgc caacctgcgt gataactatc gtctggttga tggcgcatcg
     6241 aaagacggct ggttattcac tgctgaacat actcagagtg tcctgaaggg ctttaacaag
     6301 tttgttgttc agtacgctac tgactcgatg acctcgcagg gtaaaggtct gtcgcagggt
     6361 tctggcgttg catttgataa cgaaaaattt gcctacaata tcaacaacaa cggtcacatg
     6421 ctgcgtatcc tcgaccacgg tgcgatctcc atgggcgacg actgggacat gatgtacgtg
     6481 ggtatgtacc aggatatcaa ctgggataac gacaacggca ccaagtggtg gaccgtcggt
     6541 attcgcccga tgtacaagtg gacgccaatc atgagcaccg tgatggaaat cggctacgac
     6601 aacgtcgaat cccagcgcac cggcgacaag aacaatcagt acaaaattac cctcgcacaa
     6661 caatggcagg ctggcgacag catctggtca cgcccggcta ttcgtgtctt cgcaacctac
     6721 gccaagtggg atgagaaatg gggttacgac tacaccggta acgccaatac caacactaac
     6781 ttcggcaaag ccgttcctgc tgatttcaac ggcggcagct tcggtcgtgg cgacagcgac
     6841 gagtggacct tcggtgccca gatggaaatc tggtggtaat agcaaaacct gggccggata
     6901 aggtgcttgc gccgcatccg gcatccaacg cctgatgcga cgcttgcgcg tcttatcagg
     6961 cctacaacgg ctgtcaaatg taggccggat aaggcgttta cgccgcatcc ggcataaaaa
     7021 caggttgtca ttatctgaaa ggggcgcaag cccctctgat tatcgggttt agcgcgctat
     7081 tgcctggcta ccgctgagct ccagattttg aggtgaaaac aatgaaaatg aataaaagtc
     7141 tcatcgccct ctgtttatca gcagggttgc tggcaagcgc gcctggaatc agccttgccg
     7201 atgttaacta cgtaccgcaa aacaccagcg acgcgccagc cattccatct gccgcgctgc
     7261 aacaactcac ctggacaccg gtcgatcaat ctaaaaccca gaccacccaa ctggcgaccg
     7321 gcggccaaca actgaacgtt cccggcatca gtggtccggt tgctgcgtac agcgtcccgg
     7381 caaacattgg cgaactgacc ctgacgctga ccagcgaagt gaacaaacaa actagcgtat
     7441 ttgcgccgaa cgtgctgatt cttgatcaga acatgacccc atcagccttc ttccccagca
     7501 gttatttcac ctaccaggaa ccaggcgtga tgagtgcaga tcgtctggaa ggcgttatgc
     7561 gcctgacacc ggcgttgggg cagcaaaaac tttatgttct ggtctttacc acggaaaaag
     7621 atctccagca gacgacccaa ctgctcgacc cggctaaagc ctatgccaaa ggcgtcggta
     7681 actcgatccc ggatattccc gatccggttg ctcgtcatac cagcgatggc ttactgaaac
     7741 tgaaagtgaa aacgaactcc agctccagcg tgttggtcgg gcctttattt ggttcttccg
     7801 ctccagctcc ggttacggta ggcaacactg cggcaccagc tgtggctgca cccgctccgg
     7861 caccggtgaa gaaaagcgag ccgatgctca acgacacgga aagttatttt aataccgcga
     7921 tcaaaaacgc tgtcgcgaaa ggtgatgttg ataaggcgtt aaaactgctt gatgaagctg
     7981 aacgcctggg atcgacatct gcccgttcca cctttatcag cagtgtaaaa ggcaaggggt
     8041 aattacgccc cacagtgctg attttgcaac aactggtgcg tctcctggcg cacctttttt
     8101 tatgcttcat tccagggata tgagcgattt tttatagtac ttcacttctt cttcactaag
     8161 aatatccatt atctcaatgc attatcagag attcttttcc tttcgccggt agtgtctgga
     8221 cgctcaggct actttttcca gttatttcat ttctgtaatg cagaggtttt atgttaggtc
     8281 atatctcaaa gtttgacggc aataactctt tgataaaaca tggtgtggtg caaggaaata
     8341 atatagtaga ttttga
//
LOCUS       DAZUCE010000215         8334 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZUCE010000215 DAZUCE010000000
VERSION     DAZUCE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.215"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            729..935
                     /locus_tag="ACHY03_004144"
     CDS             729..935
                     /locus_tag="ACHY03_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFS3907319.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            1011..1307
                     /locus_tag="ACHY03_004145"
     CDS             1011..1307
                     /locus_tag="ACHY03_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFS3907320.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            1313..2098
                     /gene="bet"
                     /locus_tag="ACHY03_004146"
     CDS             1313..2098
                     /gene="bet"
                     /locus_tag="ACHY03_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFS3907321.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWND
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            2095..2775
                     /locus_tag="ACHY03_004147"
     CDS             2095..2775
                     /locus_tag="ACHY03_004147"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFS3907322.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKISYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            2772..2954
                     /locus_tag="ACHY03_004148"
     CDS             2772..2954
                     /locus_tag="ACHY03_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFS3907323.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            2927..3118
                     /locus_tag="ACHY03_004149"
     CDS             2927..3118
                     /locus_tag="ACHY03_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFS3907324.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            3195..3411
                     /locus_tag="ACHY03_004150"
                     /pseudo
     CDS             3195..3411
                     /locus_tag="ACHY03_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
     gene            3510..3731
                     /locus_tag="ACHY03_004151"
     CDS             3510..3731
                     /locus_tag="ACHY03_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFS3907325.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(3942..4544)
                     /locus_tag="ACHY03_004152"
     CDS             complement(3942..4544)
                     /locus_tag="ACHY03_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907326.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWREDIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            4787..5131
                     /locus_tag="ACHY03_004153"
     CDS             4787..5131
                     /locus_tag="ACHY03_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907327.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            5256..5456
                     /gene="torI"
                     /locus_tag="ACHY03_004154"
     CDS             5256..5456
                     /gene="torI"
                     /locus_tag="ACHY03_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFS3907328.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            5724..5906
                     /locus_tag="ACHY03_004155"
     CDS             5724..5906
                     /locus_tag="ACHY03_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS3907329.1"
                     /translation="MWRDSILHSAMHSLMNSQARDTRDRSLNDDNVLEISEPCIRSTF
                     ERLYQNMNEVFGLGDY"
     gene            complement(5986..7233)
                     /locus_tag="ACHY03_004156"
     CDS             complement(5986..7233)
                     /locus_tag="ACHY03_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFS3907330.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(7305..8219)
                     /locus_tag="ACHY03_004157"
     CDS             complement(7305..8219)
                     /locus_tag="ACHY03_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFS3907331.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICAMAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
BASE COUNT         2480 a         2001 c         1849 g         2004 t
ORIGIN      
        1 gaggagattg aagattatgt taaaaaagaa gcgtttgttc aattcaaggc atgcgacgtt
       61 gacaatttaa aaacccagta taggaaacta tttcttgcct acgacttgtt gattcatgga
      121 aatgttgaaa agataaatta aataaacgcc ccgtacaaag cgatggcggg gctaataagt
      181 catttatgta tcatttagca actgactaaa aaaatacaca taagaactca gactaccaat
      241 attcgattaa atttgctgcc aacctaccga gctcaccaca acccgatgcc cctctgtcac
      301 gaggcacatt aaagctgtac aaatttcagc cacaccaata agtcttcata ctctttttta
      361 taaaaattca tttaattatc aatcagaaca agtatatcaa acattatcaa tactaattgc
      421 tattgactac accaatagcc attgctattg tcatgctatt gcatcgatgt tggtatcaca
      481 aaatttataa cttcaccgtt gcgatgaccg cttagatctg cagcttgaat ttcagcaggc
      541 tacggggagt gcgaggggtg aaacggacgt gtgaacgtcg gtgtgaccag ctgaaatcaa
      601 ctcaacacct catacctcag tcgcttcaac gaggcggctt agttatgaca accggcggcc
      661 atccaccgcc tgaatacgcg cagaagtctc tatatgttca gcagcccagc ttacgggcag
      721 gagtttttat ggttcatcaa cattacggaa cgcagaccgt taatcgaggt gcggtcatgc
      781 caggaatgct ggtcaaacac aaagatggta cctggactgc atcagctaat ttacgcggac
      841 ggctatatct gcatcgcggc atcgagcgca cttatacccg tgatttgctc gtggaagttt
      901 ttctcgacgg acgcggtaac ggcctgaatc actaatcccc tttcctgttt tcctaatcag
      961 cctggcattt cgcgggcgat attttcacag ccattttcag gagttcagcc atgaacgctt
     1021 attacattca ggatcgtctt gaggctcaga gctgggcgcg tcactaccag cagatcgccc
     1081 gtgaagagaa agaggcagaa ctggcagacg acatggaaaa aggtctgccc cagcacctgt
     1141 ttgaatcact ctgcatcgat catttgcaac gtcacggggc cagcaaaaaa gccattaccc
     1201 gtgcgtttga tgacgatgtt gagtttcagg agcgcatggc agaacacatc cggtacatgg
     1261 ttgaaaccat tgctcaccac caggttgata ttgattcaga ggtataaaac ggatgagtac
     1321 agcactcgca acgctggcag ggaagctggc tgaacgtgtc ggcatggatt ctgtcgaccc
     1381 acaggaactg atcaccactc ttcgccagac ggcatttaaa ggtgatgcca gcgatgcgca
     1441 gttcatcgca ttgttgatcg tcgccaacca gtacggcctt aatccgtgga cgaaagaaat
     1501 ttacgccttc cctgataagc agaacggcat cgttccggtg gtgggcgttg atggctggtc
     1561 ccgcatcatc aacgaaaacc agcagtttga cggcatggac tttgaacagg acaacgaatc
     1621 ctgcacatgc cggatttacc gcaaagaccg caatcatccg atctgcgtta ccgagtggat
     1681 ggatgaatgc cgccgcgaac cattcaaaac ccgcgaaggc agagaaatca cggggccgtg
     1741 gcagtcgcat cccaaacgga tgttacggca taaagccatg attcagtgtg cccgtctcgc
     1801 cttcggattt gctggtatct atgacaagga tgaagccgag cgcattgtcg aaaataccgc
     1861 atacactgca gaacgtcagc cggaacgcga catcactccg gttaacgatg aaaccatgca
     1921 ggagattaac actctgctga tcgccctgga taaaacatgg aatgacgact tattgccgct
     1981 ctgttcccag atatttcgcc gcgacattcg cgcatcgtca gaactgacac aggccgaagc
     2041 agtgaaagct cttggtttcc tgaaacagaa agccactgag cagaaggtgg cagcatgaca
     2101 ccggacatta tcctgcagcg taccgggatc gacgtgagag ctgtcgaaca gggggatgat
     2161 gcatggcaca aattacggct cggcgtcatc accgcttcag aagttcacaa cgtgatagcc
     2221 aagccccgct caggaaagaa gtggcctgac atgaaaatat cctacttcca caccctgctg
     2281 gctgaggttt gcaccggtgt ggctccggaa gttaatgcta aggcgctggc atggggaaaa
     2341 cagtacgaga acgacgccag aactctgttt gaattcactt ccggcgtgaa tgttactgaa
     2401 tccccgatca tctatcgcga cgaaagtatg cgcaccgcct gctctcccga tggtttatgc
     2461 agtgacggca atggccttga gctgaaatgc ccgtttacct cccgggattt catgaagttc
     2521 cggctcggtg gtttcgaggc cataaagtcg gcttacatgg cccaggtgca gtacagcatg
     2581 tgggtgacac gaaaagatgc ctggtacttt gccaactatg acccgcgtat gaagcgtgaa
     2641 ggactgcatt atgtcgtggt tgagcgggat gaaaagtaca tggcgagttt tgacgagatg
     2701 gtgccggagt tcatcgaaaa aatggacgag gcactggctg aaattggttt tgtatttggg
     2761 gagcaatggc gatgacgcat cctcacgata atatccgggt aggcgcgatc actttcgtct
     2821 actccgttac aaagcgaggc tgggtatttc ccggcctttc tgttatcaga aatccactga
     2881 aagcacagcg gctggctgag aagataaata ataaacggga ggcggtatgc acaaagcatc
     2941 tcctgttgag ttaagaacga gtattgagat ggcacatagc cttgctcaaa ttggagtcag
     3001 gtttgtgcca ataccagtag aaacagacga agaatttcat acgttagcca catccctttc
     3061 acaaaagctg gaaatgatgg tggcgaaagc agaagcagat gagagagacc aggtatgaca
     3121 accactgaat gcatttttct ggcagcgggc ttcatattct gtgtgcttat gcttgccgac
     3181 atggggcttg tttaatgaca cctcagcaag aaaacgccct tcgcagcatt gcccgtcagg
     3241 ctaattctga aatcaaaaaa agccagacag cagtttccgg ataaaaacgt cgatgacatt
     3301 tgccgtagcg tactgaagaa gcaccgcgaa acggtaacgc tgatgggatt cacaccaact
     3361 catttaagcc tggcgatcgg catgttaaac ggcgtcttta aggaacgatg aacatgaaaa
     3421 gcaaaattat cagggagcta caggctcctt ttttattgtt cgcattcacc ctcaagcgta
     3481 ttaaccaaca gttcagggat taatgaaaga tggcagacat cattgattca gcatcagaaa
     3541 tagaagaatt acagcgcaac acagcaataa aaatgcgccg cctgaaccac caggctatat
     3601 ctgccactca ttgttgtgag tgtggcgatc cgatagatga acgaagacgc ctggccgttc
     3661 agggttgtcg gacttgtgca agttgccagg aggatctgga acttatcagt aaacagagag
     3721 gttcgaagtg agcgaaatta actagaagcc aaagataaaa tcatcgctga gcatgagaaa
     3781 atcgctaacg gagaaaagac agtaagtcag tatatgaaaa ccgcatgata tcatcagata
     3841 aaaatcgatc gtaaagcgaa atattaatac cagaataaac gagtcgaggt aaattatatt
     3901 acctcgataa attaactaaa acttgcccgc tatatactat ctcattcagt atcatcacgc
     3961 gcggtctgtg catatgtcac taccgcacct aatgtattaa ttttcttttc aacatagata
     4021 atattatcgt actcataatt gccatacgga tagcaaatgc gaatattctc atgtagatcg
     4081 gggtcatcca cctcagctcc agaacaactt tttgaactac cggaagtata ccgatacggt
     4141 gcaacataag acgatgtctc tccaggcaaa aaataagtta gtgttgtaag gggtataatc
     4201 agaaaaaatc cagaaaatat gcacatccct gcataaacct taaggtatgc tgacagactc
     4261 ttccagccgc tttgttttac tatccccttc ttaacccaaa acagagataa cagaaaagct
     4321 attcccatgc taaacagaat gtaatagtgg gatatactct gattaagaaa cgtgaccctg
     4381 tagatatctt cccgccacca gaagaaaagg aaaataaaga tcagccctga aactgtcatg
     4441 caaatcaaat aaggatacga atcttttttc atgtttagcg cccataaaat ttttcctgcc
     4501 ccggacaaat ttaccatcca ttttttgcgc agaaaatagc tcattactta ctgcacaata
     4561 atacacaaaa ttgcgtaaat tttttgcatg gattttagct ctttcagccg acgtttaagg
     4621 ggtaaatagc atttcctaaa agcaactgca ccaacccaac agaatgggct accgcttacg
     4681 ttgagagcaa aaaagtgtat agcagcaatg aacagcatcc tcgcactgac ggggatttct
     4741 tttatctgag ctcgctacgg cgagttttgt tttatggaga caagaaatgt cagatttggc
     4801 tatgaaggtt ttgaaatggc aatcgactgg cgatgtcggc atcagtagcg caactcttgc
     4861 ctcaatcgca tgtggactga aaaagaatat ctatggtcat agcttcggtg ctccacatga
     4921 cgcagccgat ttccggcgat gcgttgcact tgttgagcag attccagaaa tcagagattc
     4981 attcgacaag gttgcaaagc gcgttccggc attcaaaggc atcctcaacg aatgggattc
     5041 tctcgttgct ctgttgaagt ctgaaatgaa gatacacgga aacaaagcac cagagactta
     5101 cagaagaatc agcgagctac gcaaggacta accacagtct cacactcgat gaggcctgtt
     5161 catttctcaa gatatccaga cctaccattg ctgcatcaat gcggcttttt attgcctgat
     5221 ttgcaggttc gattccctat tcggagatag cactcatgca acacgaacta cagcctgatt
     5281 ccctggttga tttgaaattc atcatggctg atactggctt tggtaaaacc ttcatctacg
     5341 accggattaa gtccggcgac ctgccaaaag ccaaagttat ccacgggcga gcaagatggt
     5401 tatatcgtga ccattgtgaa ttcaaaaata agctcttaag ccgcgccaat gggtaaaata
     5461 gcgggtaaaa tatttctcac acctaaaaaa caccattcca atcaatcccc tgccgcttca
     5521 agtagatgtc tgcaggggac accagatacc cttcaaacga aatctacctt caccccgtaa
     5581 aagatgggtt tggcagcaca cttgccctat atctactcat ttttactgca acaggttgaa
     5641 atctcagcac tgtcagaaag cgctgatgac taaacagcct tgggccgggc gatgtaacca
     5701 tcacacagaa tcctgatagc gaaatatggc gtgactcgat acttcactcc gcaatgcatt
     5761 ccttgatgaa ttcgcaggcc cgtgatacac gggacaggtc gctgaatgac gacaatgtcc
     5821 tggaaatcag cgaaccgtgt atccggagta catttgagcg actgtaccag aacatgaatg
     5881 aggtgtttgg attaggcgat tattagcagg gctaagcatt ttagtattat tattttccgg
     5941 ttgaaggata tggagatatc gacaacgacc ggaaaaagtt tacgtctata ttgctgaagg
     6001 tacaggcgtt tccataacta tttgctcgcg ttttttactc aggaagaaaa tgccaaatag
     6061 caacatcagg cagacaatac ccgaaattgc gaagaaaact gtctggtagc ctgcgtggtc
     6121 aaagagtatc ccagtcggcg ttgaaagcag cacaatccca agcgaactgg caatttgaaa
     6181 accaatcaga aagatcgtcg acgacaggcg cttatcaaag tttgccacgc tgtatttgaa
     6241 gacggatatg acacaaagtg gaacctcaat ggcatgtaac agcttcacta atgaaataat
     6301 ccaggggtta acgaacagtg cgcaggaaag gatacgcaac gccataatca caacaccgat
     6361 aagtaatgca ttttttggcc ctacccgatt cacaaagaac ggaataatcg ccatgcacag
     6421 cgcttcgagt accacctgga atgagttgag ataaccatac aggcgcgttc ctacatcgtg
     6481 tgattcgaat aaacctgcat aaaagacagg aaaaagttgt tgatcaaaaa tgttatagaa
     6541 agaccacgtc ccaacaataa atatgacgaa aacccagaag tttcgatcct tgaaaactgc
     6601 gataaaatcc tcttttttta cccctcccgc atccgccgct acgcactggt gatccttatc
     6661 tttaaaacac atgttgatca tcataaatac agcgccaaat agcgagacca accagaagtt
     6721 gatatgggga ctgatactaa aaaatatgcc ggcaaagaac gcgccaatag catagccaaa
     6781 agatccccag gcgcgcgctg ttccatattc gaaatgaaaa tttcgcgcca ttttttcggt
     6841 gaagctgtca agcaaaccgc atcccgccag ataccccagg ccaaaaaaga gcgcccccag
     6901 aattagacct acagaaaaat tgctttgcag taacggttca taaacgtaaa tcataaacgg
     6961 tccggtcaag accaggatga aactcataca ccagatgagc ggtttcttca gaccgagttt
     7021 atcctgaacg atgccgtaga acatcataaa tagaatgctg gtaaactggt tgaccgaata
     7081 aagtgtacct aattccgtcc ctgtcaaccc tagatgtcct ttcagccaaa tagcgtataa
     7141 cgaccaccac agcgaccagg aaataaaaaa gagaaatgag taactggatg caaaacgata
     7201 gtacgcattt ctgaatggaa tattcagtgc cataattacc tgcctgtcgt taaaaaattc
     7261 acgtcctatt tagagataag agcgacttcg ccgtttactt ctcactattc cagttcttgt
     7321 cgacatggca gcgctgtcat tgcccctttc gccgttactg caagcgctcc gcaacgttga
     7381 gcgagatcga taattcgtcg catttctctc tcatctgtag ataatcccgt agaggacaga
     7441 cctgtgagta acccggcaac gaacgcatct cccgcccccg tgctatcgac acaattcaca
     7501 gacattccag caaaatggtg aacttgtcct cgataacaga ccaccacccc ttctgcacct
     7561 ttagtcacca acagcatggc gatctcatac tcttttgcca tggcgcatat atcccgatcg
     7621 ttctgtgttt ttccactgat aagtcgccat tcttcttccg agagcttgac gacatccgcc
     7681 agttgtagcg cctgccgcaa acacaagcgg agcaaatgct cgtcttgcca tagatcttca
     7741 cgaatattgg gatcgaagct gacaaaacct ccggcatgcc ggatcgccgt catcgcagta
     7801 aatgcgctgg tacgcgaagg ctcggcagac aacgcaattg aacagagatg taaccattcg
     7861 ccatgtcgcc agcagggcaa gtctgtcgtc tctaaaaaaa gatcggcact ggggcggacc
     7921 ataaacgtaa atgaacgttc cccttgatcg ttcagatcga caagcaccgt ggatgtccgg
     7981 tgccattcat cttgcttcag atacgtgata tcgactccct cagttagcag agttctttgc
     8041 attaacgcac caaaaggatc atcaccgacc cgacctataa acccacttat tccgcctaat
     8101 ctggcgattc ccaccgcaac gttagctggc gcgccgccag gacaaggcag taggcgcccg
     8161 tctgattctg gcaagagatc tactaccgca tcccctaaaa cccatacttt ggctgacatt
     8221 tttttccctt aaattcatct gacttacgca tagtgataaa cctctttttc gcaaaatcgt
     8281 catggattta ctaaaacatg catattcgat cacaaaatct catagttaac gtta
//
LOCUS       DAZUCE010000216         8247 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZUCE010000216 DAZUCE010000000
VERSION     DAZUCE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.216"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            178..696
                     /locus_tag="ACHY03_004158"
     CDS             178..696
                     /locus_tag="ACHY03_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFS3907332.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVIHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(741..2786)
                     /gene="dcp"
                     /locus_tag="ACHY03_004159"
     CDS             complement(741..2786)
                     /gene="dcp"
                     /locus_tag="ACHY03_004159"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFS3907333.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMLQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAHADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVENRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2923..3669
                     /gene="ydfG"
                     /locus_tag="ACHY03_004160"
     CDS             2923..3669
                     /gene="ydfG"
                     /locus_tag="ACHY03_004160"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFS3907334.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3758..4444
                     /gene="rspR"
                     /locus_tag="ACHY03_004161"
                     /gene_synonym="ydfH"
     CDS             3758..4444
                     /gene="rspR"
                     /locus_tag="ACHY03_004161"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFS3907335.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4621..4824
                     /gene="ydfZ"
                     /locus_tag="ACHY03_004162"
     CDS             4621..4824
                     /gene="ydfZ"
                     /locus_tag="ACHY03_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFS3907336.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4860..6320)
                     /locus_tag="ACHY03_004163"
     CDS             complement(4860..6320)
                     /locus_tag="ACHY03_004163"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFS3907337.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(6409..7692)
                     /gene="ydfJ"
                     /locus_tag="ACHY03_004164"
                     /pseudo
     CDS             complement(6409..7692)
                     /gene="ydfJ"
                     /locus_tag="ACHY03_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT         2194 a         2079 c         1885 g         2089 t
ORIGIN      
        1 aatcagccaa ctagctgtca aatattcaga gagtttaact cactaaagtt aagaagattg
       61 aaaagtctta aacatatttt cagaataatc ggatttatat gtttggaaat tattatattg
      121 gacgagcata cagaaaaagc aaatcacctt tacatataaa agcgtggata aaaaacagtg
      181 aacattaata gaaataaaat tgtacaactt gcagataccg acactattga aaacctgaca
      241 tccgcgttga gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc
      301 accggcggta agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt
      361 gcaggctttg ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcaa
      421 tctcttgaac gatattctgc ggtgagtgag aaagtggcag cagaaatggc aaccggtgcc
      481 atagagcgtg cggatgctga tgtcagtatt gccattaccg gctacggcgg accggaggga
      541 ggtgaagatg gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac
      601 tacactgcgg ttatacattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg
      661 tttgccctcg cccagctgct gcagttactg ctataaccag gctggcctgg cgatatctcc
      721 aggacagcca ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc
      781 tgaggtgcct taccgcgcca ttgtcgatac aggcgttcca gatcttcgct gttacctctg
      841 gaaaggatcg cctcgcgaaa acgcagccca ttttcacgcg ttaatccgcc ctgctcaaca
      901 aaccactgat aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgcg
      961 gcatatccgc caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca
     1021 ggaagatcca tattttccgc caccagcgcc cgcaattcga aatcatcgac atcctgcatt
     1081 gcttcgtttt cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg
     1141 ctcatctcat accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca
     1201 ggcattgctg ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt
     1261 gcccagtgtt cgttgatctg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg
     1321 gagagcgtgg cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg
     1381 aataaggtta tgacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga
     1441 taattgcaga cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa
     1501 ttgcccatcc atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac
     1561 gccagcccca cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca
     1621 ggaatatcaa aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca
     1681 ccttcattca acaccgtgtt taattcaaaa tatggcttga gctgcgcctc atcaagatca
     1741 aatttctccc gccggacctg ttcggcataa aatgcccagt cccacggctg cgcgctaaac
     1801 ccgccctgct gcttatcaat aaccgcctgt atggaggcta attcatcgct cgcacgttga
     1861 cgcgccgctg gaacaatttc ccgcataaag ttgagtgctg cttcaggtgt ttttgccatc
     1921 tgatcggcga ttttccatgc ggcataatga ggaaaaccga gtaatgtagc ctgttgtgca
     1981 cggttctcca ccagacgttg aatgatagcg cgggtatcat tggcatcatt tttttccgct
     2041 cgcgtccagc ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc
     2101 gccggttgtt gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg
     2161 cgagccgcct ctgccgccag cgcaatctct tgctcactca ttcctgccag ttgcgcgata
     2221 tcgttcacaa ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg
     2281 gtcagggtcg cagcttctgt attcagtact tttaattttg ctttatcagc atgcgcaagc
     2341 ttggctccgg caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta
     2401 tcaagcccca gagattcacg gcgttgccag acagcatcta cccgcgcgaa taattcacca
     2461 ttcagataga tatcattagc cagttccgcc agttcagcgg aaaactgctc gtcaagacgc
     2521 tgtaattcat cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta
     2581 agtaattctc cgctttgttc cagtgccaga atagtattgt tgaaatcagg tgtttgcggg
     2641 ttaagcgcga tggcagcaat ttctgcccgc ttttgcagca ttccctcatc gaatgccggg
     2701 cgatagtgat gattggcaat ttgatcaaaa tggggagcca gatacggcag tgtgctttgc
     2761 acaaggaaag gattcattgt tgtcattttc ttctcctgaa cgcgaggtgt tccatagcgt
     2821 aggcttactg ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac
     2881 gtgctatgtt attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact
     2941 ggagcaacgg caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa
     3001 gttatcgcca ctggccgtcg ccaggaacgg ttgcaggagt taaaagacga actgggagat
     3061 aatctgtaca tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca
     3121 tcgctccctg ccgagtggag caatattgat atcctggtaa ataatgccgg cctggcgttg
     3181 ggcatggagc ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac
     3241 aataaaggcc tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat
     3301 ggtcatatta ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt
     3361 tacggtgcga cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggatctgcat
     3421 ggtacggcgg tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc
     3481 aatgtccgct ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca
     3541 ttgacgccag aggatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc
     3601 aatatcaata ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac
     3661 cgtcagtaat ttttataccc ggcttaactg ccgggttatt gcttgtcaca aaaaagtggt
     3721 agactcatgc agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa
     3781 tcccacacag cctgtcaatc agcagattta tcgtatcctt cgtcgcgaca ttgtccattg
     3841 cctgattgct ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc
     3901 acgccagccg gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg
     3961 tccgcagcgt ggcagctacg tcaacaaaat ttccatggcc caggtgcgca acggcagttt
     4021 tatccgtcag gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag
     4081 ccagtgctac caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca
     4141 actggatgat ttttttgaac ttgatgacaa cttccatcaa ctcttgacgc agattgccga
     4201 ctgtcaactg gcgtgggata ccattgagaa cctgaaagca accgttgatc gcgtgcgcta
     4261 tatgagtttc gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt
     4321 ctctgccctg caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca
     4381 ggaaatcagc gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga
     4441 gtaattcatt tcctctcatc ccatccggga tgagagtctt tacccccgcc ttatggctca
     4501 tgcatgcatc aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga
     4561 gtatttatat tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaacacct
     4621 atgacgacct acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc
     4681 ggcaccggtc acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa
     4741 atccgccgcg gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc accactggac
     4801 ctgattcgtc tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt
     4861 tacttcacgg aatattttgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac
     4921 gcttccgtca cttttgcagt aaacaagcta ttgtctggca aatcaccacc aaaaatcgcc
     4981 ttaatcgcca gcaatgactg gacgcgcgct ttcccttcgg cactactttg tacagccttc
     5041 tgaataacag gtaacagtgg gtcactgatt tctatcggat ttccctgttc atcaacacca
     5101 ccgacataac gcatccaacc cgcgacgccc agcgccagca gatcgaactt gctgtcatgc
     5161 gccagatgcc agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc
     5221 gcaatctgcc aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta
     5281 gcgtaatctt gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc
     5341 aagccatacg ccgcataacg ataatgttca tcttccatac agtcattaat gtgctgatat
     5401 ccggcaagat aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc
     5461 atctcttcat aaggcagtac atcgctaacc agttcggctc ccgctttttc ccattccgga
     5521 cgtccggcaa caaaattatc ttcgattacc cactggcgga aaggttcaca ggcaacgcca
     5581 gcaggatcgc gcacaccggt aagctgttcg attttcgcca gcgtatcctc tgtcactgcg
     5641 ggcacaatac ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc
     5701 agttttacat caacggcttg cgcgtaggaa gtgacaacgt cacgcataac atgaccgttt
     5761 tctggcatgt tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcga
     5821 gccagcgcct caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg
     5881 gcagcgacca tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct
     5941 ttttcggtga ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg
     6001 gtttctaagc catctatctg tacgtgcaag gcttttttaa cgacgccaac gacgcgagcc
     6061 gtccacgcat cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg
     6121 gcaatttgct gttcgccacc gattaagttg acctcataat atccccagtc actgaaatgt
     6181 tctgtagcaa gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct
     6241 aaatgaacaa ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct
     6301 gataacaaat tatttcccat aacaattcct taaatttaaa tatggcaagc tacatgtttt
     6361 gttatatgga taaaaatccc ctctccggta agagaaggga ttgaggattt acagacttct
     6421 ggaaggttgc gcagctctta caacacgcgg ttgatcttcc gcagcgtctt ccagcgcact
     6481 taaatcacgg tctttcactt ctggcatttt cagcgcagag attaaaccaa tcactgaata
     6541 tgccatgatc ataatggcga ttggatacca ggattccgtc atggtgcaga aaatacctgc
     6601 caggatagga ccaaaaccgg aagcgataag accaccaatt tcttttgaaa tagccatccg
     6661 ggtaaagcgg tttttacagc cgaacatttc tgccatggta atgttttcca gagcaaataa
     6721 tcccagcacc gcacagttat gaatcacaat cagtgcaacc ataatggtgc tcggggcata
     6781 gcttttatct acgatgatag aaagcattgg ccatgccagc acaatcgcgg aggtattcat
     6841 aataatatac gggatccggc gaccaatttt atcggataac caaccaagga acggaatggt
     6901 cataaagccg agaatcgaac tgatcatcaa tgcatctgtt ggaattgctt tgttaaacaa
     6961 taacgtctgt actaaatagc ctgcaaggaa agtctgaatt aacccggagt tacctgcctg
     7021 accaaaacgc agtcctgttg ccagccagaa ggatttgctc tggaacatgc taccagcagg
     7081 tgcaggtttt gctgtcggtt ggttgctgtc gttaactttc tcaaagaccg ggctttcttt
     7141 cagattcata cgcaaccaga tagcaaagac catcacgaca acgctcgcca ggaacggtat
     7201 acgccatccc cacgccagca gttcctcttt actgagaatg aagaacataa aggcccagat
     7261 tgccgttgcg ctcaaggttc cgcagttagt tcccatagct acaaatgagg agataattcc
     7321 gcgcttacct tttggcgcat attccgccag catcgtaccg gcaccggaaa tttccgcacc
     7381 tgcacccaac ccctgaataa tacgcagcgt caccagcaag atgggtgcaa aaacaccaat
     7441 ctgtgcatag gtcggtaaca ctccaattaa ggtggtacag atccccatca tggtgatggt
     7501 aataaagagc acttttttac gccctattct gtcgcccatt ttgccgaaaa taaatgctcc
     7561 gacaatacgc gccacataac ctgcaccgta ggttcccatt gccaaaatta acgccattgc
     7621 cgttgatgat tcaggaaaaa atatttcatg aaacactaac gctgcgccga gcgaatataa
     7681 ctggaaatcc ataattcaca ggtgtttttt cccatcctgt ggtttccttg gcgttttcta
     7741 ggttttttca gatagttgca ttttttaaaa agcatcctaa gttcgatctc agtgtctatc
     7801 tggggcctat ttctgtccca tatatgcccc aaaaaaaact ccccaacaga taagtagttt
     7861 tttcatggat ttatgcgtaa aatcaagaac ggctggaaat cattcaatac tcacactatc
     7921 gaaaaattta ccagccaacc gcagcacgtt cttgcataag gcgtgtctgc ggtttttcaa
     7981 ctattcagat acatcactcc catcacattc attcctccgc atcaaaggca tataggctat
     8041 atcaccttga tatttttctt tttcagataa aaactgttat ctatgtatac ttttaaaccc
     8101 aatccgtgta gagtctctgc ataagatagt ttgcagttgc cgcttcagct tgcgccataa
     8161 accgcctgat ttttgctgcc acctgttagc attcctgtat acctgaaacg acaatgttta
     8221 tctacgaact ttaagaacac ccaggat
//
LOCUS       DAZUCE010000217         8242 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZUCE010000217 DAZUCE010000000
VERSION     DAZUCE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.217"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(216..866)
                     /gene="zinT"
                     /locus_tag="ACHY03_004165"
     CDS             complement(216..866)
                     /gene="zinT"
                     /locus_tag="ACHY03_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFS3907338.1"
                     /translation="MAIRLYKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1123..1758)
                     /gene="msrQ"
                     /locus_tag="ACHY03_004166"
     CDS             complement(1123..1758)
                     /gene="msrQ"
                     /locus_tag="ACHY03_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFS3907339.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTLAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(1759..2763)
                     /gene="msrP"
                     /locus_tag="ACHY03_004167"
     CDS             complement(1759..2763)
                     /gene="msrP"
                     /locus_tag="ACHY03_004167"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFS3907340.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTGRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPDQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2872..3285)
                     /gene="hiuH"
                     /locus_tag="ACHY03_004168"
     CDS             complement(2872..3285)
                     /gene="hiuH"
                     /locus_tag="ACHY03_004168"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFS3907341.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3418..4089
                     /gene="hprR"
                     /locus_tag="ACHY03_004169"
     CDS             3418..4089
                     /gene="hprR"
                     /locus_tag="ACHY03_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFS3907342.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4089..5447
                     /gene="hprS"
                     /locus_tag="ACHY03_004170"
     CDS             4089..5447
                     /gene="hprS"
                     /locus_tag="ACHY03_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFS3907343.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(5555..6406)
                     /gene="hchA"
                     /locus_tag="ACHY03_004171"
     CDS             complement(5555..6406)
                     /gene="hchA"
                     /locus_tag="ACHY03_004171"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFS3907344.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(6998..8185)
                     /gene="ompC"
                     /locus_tag="ACHY03_004172"
     CDS             complement(6998..8185)
                     /gene="ompC"
                     /locus_tag="ACHY03_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFS3907345.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVAGLHYF
                     SDDSGSDGDMSYARIGFKGETQIADQFTGYGQWEFNIGANGPESDKGNTATRLAFAGF
                     GFGQNGTFDYGRNYGVVYDVEAWTDMLPEFGGDTYAGADNFMNGRANSVATYRNNGFF
                     GQVDGLNFALQYQGNNEKSGLFDQEGSGNGNGRKLAKENGDGYGMSTSYDFDFGLSLG
                     AAYSNSDRTDNQVHKGTHNTRYGDRFDATAGGETAEAWTVGAKYDANNVYLAAMYAET
                     RNMTGYGDADAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGVNSDNFDSQGN
                     HHYTNKDLVKYVDIGMTYYFNKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVGLV
                     YQF"
BASE COUNT         2258 a         1749 c         1917 g         2318 t
ORIGIN      
        1 gcaatggcgt gagcacaggt atctttctct gttggccgta ttgtgtatgg aatccgttat
       61 tgattaactc aatgcgggtt agcttattaa tatacagaca cgacatagtg atgagcgtgt
      121 ctgtagctag cttgcgttca gtggcgtttg tggatggacc agtggcagtt atgatgactt
      181 actaaagcgg catcgaggcg ttatcatgag aatactcaat gagacatcat ttcctcgacc
      241 acttcttcgc tactcaactg atatggataa tacgttggcc agttttccat ttcatttaat
      301 aaagattgct gtgaatcatt acccataaaa atgtgaaaat gagatgattt tcgtggtgca
      361 attatatgat cgctaaattg tatatattta ggggctttgc tttcaggatc tttacattcg
      421 aataagtaac gaacgccttt cttgcctgat ttataggtga gtattttgta tccatcgtaa
      481 tcatatttac aggatgttgt ttcattattt ctatggaatt caacaatgcc gtcctcaatg
      541 ccaatcatct cgatatctgt tgcataacct ttgtgataat aatctttaat ttcagcaaat
      601 gttttagttt tatctgcatc cgctttcttc tgaaagacgg ggtcaagttt gccactttgc
      661 agtaaaggat aaacggattg ccagactcca tcccagtcac tgagcgttcg gttttgtaca
      721 ttggcatcat caaaaacacc attagcagct ttttgttcga cctctgttaa gggtttgccg
      781 tgtgagtgat gaccatgcga aaaggcagga gcgctaacaa taaagacacc taaagcaaca
      841 gccagtttgt aaagacgaat cgccaaaaca gtttcctcca gagttaatgt atatgatgtg
      901 taatgttata ttgtaacata tagcaaaatg caacaactct taggaaacga gagcatggct
      961 ttctcagttc tacttatcta tcttattgaa ataaagtaaa ttattttggt gatttgaggt
     1021 tgatgacaaa cgaaacataa cctgatgcga tctgtgtatg ccggataggg cgctcgcgcc
     1081 acatccggca gggataacgc gtactttgtc ggcaatatga agttatacag ataatttgtt
     1141 gtgaacctgc ttgcgtaacc ggttaaaaag ggaacgcaac ttcttatacc gtaaggctaa
     1201 aagcagtaca gccagcccag cgtagatgag cggctgcggt gaaataatct tcactgacca
     1261 cagataatgt attggggcca ggatcgcgac aagatagacg aaattatgca actgttgcca
     1321 gtgcttgccg agttttcgct gcatcgccag ggttgaagta aacgctaaag caagcaaaat
     1381 aacccagctg ataattccta acgttaaata aggccgggta attaactcct tgcctaataa
     1441 cgccagatta ttcacgccca gctccagcaa tgcgtaactg gttaaatgca gtgtcgccca
     1501 ggcaaagcac cataagccta acaggcggcg agtgcgtatc aataacggct gttttgcgta
     1561 gcgtgctaga ggtgtgatta acaaggtcgc cagcaaaaat ttcagtgcag tgcgaccggt
     1621 aaaatgctga atatctttca ccggatcggc acccagtcca ccgtgattga tcgcccagac
     1681 cagccagaga aacggcaaca atccggcaag atgcaggcaa actttcagcc atgtcacctg
     1741 ttttgctgtc agacgcattc agaaattctc ccgcaaatcc aggccacgat acagcgatgc
     1801 cacctggtcg gcgtaaccat taaacagtag cgttggctgg cgctgtacat cgaggatgcc
     1861 gcctgaacca ataaatcgtt cggtagcctg tgaccagcgc gggtgatcaa catgcggatt
     1921 aacgttggcg taaaaaccgt attcgtcagg cgctgccaga ttccaggtgg ttggcggacg
     1981 ctcgcgggtc agcttaatac tgactatcga tttaatccct ttaaagccat atttccacgg
     2041 cacaatcagt cgcactggcg cgccattttg tggcggtaac gccttgccat aaacacctac
     2101 ggtcatcagt gtgagcggat gcattgcttc gtcgagacgc aatccttcga cataaggata
     2161 tttcagccca ccgccgataa agcggtcctg ctggtctggc atctgttccg gtgcataaat
     2221 tgtttcgaaa gcgacatact tcgcattgct ggtgggttcg gcaagcgcca gcaatttgtg
     2281 cagcggaaaa ccaatccacg gcaccaccat cgaccacgct tccacgcagc gcatacgata
     2341 aatacgctct tccagcggga agcgaccggt taagtcatca tgatcgaggg tcaatggttt
     2401 tgccacttcg ccgctgattt tcagtgtcca tggatcggtt ttcaggctac cagcattagc
     2461 ggcgggatcg gctttatcca gcccgaattc atagaagttg ttataaccgg aaactttatc
     2521 tgctggcgtc agtggcaggt tattttgcca ggcggcaggc ttgctgaact ccagcgcttt
     2581 tccggcgggg gcgggcgggc gatcgttccc tttaaaccag ctaagcagat cggcatgcgc
     2641 agcgtgaggc aaagaaagtg cagctgcact gatgcccagt gcttttaaca cctgccgacg
     2701 cttcataaag aataccgact cggccgtaac atctgattct tttaaaaatt gattcttttt
     2761 cataagccat cacacttttt tgtcgttagc cagcaagagc cgtttactgt taatctgtca
     2821 gggagtgtcc tccctaacag tagcagagat ctgcttttac atatttatgc attaactgcc
     2881 acgataggtt gaatacccat attggctaag taataaaggc acatgataat gctcgttcac
     2941 tttattaata tgaaattcaa cggggatctc agggaagaaa ctttcaagat tttgtttctt
     3001 gaaatagtcc ccggttttaa atacgacacg gtaatcgccc gtagttgcag tttgctcggg
     3061 ccacagtgcc ttaattcgtc catccttatc tgttttggcg gtattaagtt gtaaccagcc
     3121 gttgtccgct ttcttttcaa gagtgacagt cacgtcggca gcgggttttc ctgtttgctg
     3181 gttcaaaatg tgcacgctaa gaatgttttg ttgtgcagca tagaccaaag aaggtaatga
     3241 aaatgcagcc gttactacgg agagtactaa ataacgcttt aacatgttta tatccttgtc
     3301 atgtgaatga gttttgagga tagcgaaaaa gcgtccaggg taaccttaca gcagcattac
     3361 aattttgtaa tgaaccaggc tgtttctata acatatgatt tatggcatat tattttcatg
     3421 aagattttac ttattgaaga taatcaaagg acccaggagt gggtaacgca ggggctttcc
     3481 gaagcgggtt atgtcattga tgccgtttct gatggcagag atgggcttta tcttgcgctg
     3541 aaggatgatt atgcattgat cattctggat attatgcttc cgggtatgga tggctggcag
     3601 atcttacaaa cgttaagaac agcaaagcaa acccctgtta tttgccttac tgcaagggat
     3661 tctgtcgatg acagagtcag agggctggac agtggggcaa atgattatct ggtaaaacct
     3721 ttttcatttt ctgagttgct ggcaagggtt cgggcacaat taaggcaaca tcacgctttg
     3781 aattcaacat tagaaatcag cggcttaaga atggactctg ttagtcaaag tgtgagcagg
     3841 gacaatatca gtattacact gacgcgcaag gagtttcagt tactttggct actggcctcc
     3901 agagctggcg aaattatacc cagaacggtt attgcgagtg aaatttgggg aatcaacttt
     3961 gatagtgata ccaatacggt ggacgtcgcc attcgcaggc tccgcgcaaa agttgatgat
     4021 ccttttcctg aaaagctaat cgccacaatc cgggggatgg gctattcatt cgtagcggta
     4081 aaaaaataat gaaaagatta tctataaccg tccgtttaac cttgcttttt atattgctgc
     4141 tgtctgttgc tggcgctgga attgtctgga ctctctataa tggcctggca agtgagttga
     4201 aatggcgcga tgatacaaca ctcattaacc ggacagcaca gatcaagcag ttgttaattg
     4261 atggggtaaa tccagatacg ttacctgtat actttaaccg gatgatggat gttagtcagg
     4321 atatcttgat cattcatggt gatggcatca ataaaattgt taaccggaca aatgtcagtg
     4381 atggcatgtt aaataacata cctgctagtg agacaatcag cgcagctggc atttacagaa
     4441 gcattattaa tgacacagag atagatgctt tacgaattaa tattgatgaa gtttcgccat
     4501 cattaacggt tactgtggct aaattggctt cagccagaca taacatgctt gaacagtata
     4561 aaatcaatag cattataatt tgcattgtcg ccattatact gtgctcagta ttaagtccgc
     4621 tgttaatccg aacggggtta cgagagatca aaaagttgag tggtgtaacg gaagcgctga
     4681 attataacga tagccgagag cctgttgagg ttagcgcatt accgagagaa ctaaaacctc
     4741 ttgggcaggc gttgaataaa atgcatcatg ctttagtcaa agattttgag cgtctaagtc
     4801 agtttgctga cgatctcgct catgaactta gaacgccaat taatgcatta ctgggtcaga
     4861 atcaggttac gctcagtcaa accagaagta tcgctgaata tcaaaaaaca attgccggaa
     4921 acattgaaga gctggaaaat atttcgcggt taacagagaa catactgttt cttgccaggg
     4981 cagataaaaa caatgttttg gtgaagctgg actcgctttc tctcaataag gaagtcgaaa
     5041 atttgttgga ctatcttgaa tatctttcag acgagaaaga gatttgcttt aaggtcgagt
     5101 gcaatcagca aatctttgcg gataaaattt tactgcaacg aatgttatcg aatcttattg
     5161 ttaacgctat tcgatattcg ccagaaaaat cgcgtattca tataaccagt tttcttgata
     5221 ccaacggatc tcttaatatt gatatcgcca gccctggaac gaaaattaat gagcctgaaa
     5281 aactcttccg tagattttgg cggggagata attcgcgtca ttccgtaggt cagggacttg
     5341 gtctttcttt agtcaaagcg attgccgaat tacatggggg aagtgctacg tatcactatc
     5401 tcaataagca taatgtgttc cggattacgt tgccgcaaag aaattaacat agggcttcag
     5461 tacgccataa attaaccgtg aaaaaacgca gccacgtcgt gagtactaac gcggccgcga
     5521 ttgattatgc gcttacattc aaacgtaaca gggattaacc cgcgtaagct gccagcattt
     5581 cctgcgccgc cagtttaccc aacgcattcg ctgcaaaagg actgtcgccg gtgagaagtt
     5641 tacggtcctt atgtactcgc ccggtgatgt cgtcattaat gatattcatg cccattttct
     5701 tcagttcttc gccgaagtac caggtgagat gacccggcat atagccaatc tctggcgttt
     5761 gtttgtctgc ggcgtctggg aatgcgcaaa tggaataacc attcagtggg ttatcgccgt
     5821 ggcgaagcgc cagaaaagcc gccgggccgt ggcaaaggga gataacaaaa cggtcatttt
     5881 tgattgccca ctgtaaagcg gcagccacgt cctggctttc aggtaaacca ataagtgcgc
     5941 catgaccacc aggaacaaag attgctgcat attcgctatc agcgttgagg ctggcgacga
     6001 catccgcgag tttcttcgga ttgcggaaca acgatttatg ctgctcaaag aatggcatta
     6061 ctttttcatc tttgtgcggc atagcccagt attcaaactt ggtcatcaga ccggaaatgg
     6121 tcgccacttc gaattcgaaa cctgcagcat ggagatgata caacggcagc aacgtttcaa
     6181 tcggatggtt accggtcgag aacagctttc cgttatcggt cggcaaataa cgttcgtccg
     6241 cggcgatcac cagaatttta tgtttaccgc gatacggttt tggatagtcc acgccatcaa
     6301 gatcagagac aggactggta tattggctaa gcgaatattc tgaagggaag aatgcattat
     6361 cttcagcaat atcgacctgc ggatttttac ttgtttgaac agtcatagtg tattccttat
     6421 tgttgcttag ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag
     6481 gctggaaaat tgagtcaata tatcgaaagc ggtagtcggc gggtgtaagt atgtaatgat
     6541 gatgctgcac tggtgttgaa tattattcac gccaaatttt ccttattgag taacgtcgtc
     6601 gcaagtggca tcaatgattt tagaccatta tcagaggtac ggggcggcgg agagagattt
     6661 agtgctgtgg aatattgtgg tgctgatgaa tatttatatc gcggattggt agagaaaaag
     6721 cttatttcat gcactatttt tgcaaatatt tctatatggg aagcgtgttt gttacggatt
     6781 aattcataaa gaaatgcatt tgggtgttag cttgaagagt agctggttga tgttattgtg
     6841 ccaggagggg caggctaagg tgctgtatcg atatgcttcg gcaattgcgt gtgccgcaga
     6901 tggagatgaa atgccaataa aaaagcctga gttcgatgag ggacgaactc aggctagtgt
     6961 tcagttttac agtaacacgc cgcggcgcgt tggctgatta gaactggtag actaagccca
     7021 cagccacgat atcatcggta gcgatgccgt tttctttgta gaagtcgtcg tcgttgtcca
     7081 gcaggttgat tttgtaatca acataagtgg acatgttttt gttgaagtag taggtcatac
     7141 caatatcaac atatttaacc agatctttgt tggtgtaatg atgattaccc tggctgtcga
     7201 agttatcact atttacaccg cctaaatcct tacctttaga ttgcaggtaa gcgatggacg
     7261 ggcgcagacc gaagtcgaac tgatactgag caacaacttc aaagttttgg gttttgttag
     7321 cgatggcatc agcatcaccg taaccagtca tgttacgggt ttcagcatac atagctgcta
     7381 agtaaacgtt attagcgtcg tatttagcac caacggtcca tgcttctgct gtttcaccgc
     7441 cagcagttgc atcgaagcga tctccataac gagtgttatg agtacctttg tgaacctgat
     7501 tgtcggtacg gtcagagtta gagtaagcag cgcccagact taaaccaaag tcaaagtcat
     7561 aggaagtgga cataccgtaa ccgtcgccgt tctctttagc aagtttacgt ccattaccgt
     7621 tacctgaacc ttcttgatca aataatccgc ttttctcgtt gttaccctga tactggagtg
     7681 caaagttcag accatcaact tgaccaaaga aaccattgtt acgataggtt gctacgctgt
     7741 tagcacgacc gttcatgaag ttgtcagcgc cagcataggt atctccacca aattctggca
     7801 gcatatcggt ccatgcttct acgtcatata cgacaccgta gttacgacca tagtcgaaag
     7861 taccattctg accaaagccg aaacctgcaa atgccagacg cgttgcggta ttacccttgt
     7921 cgctttcagg accgtttgcg ccaatgttaa attcccactg accataacca gtgaactggt
     7981 cagcgatctg ggtttcacct ttgaaaccga tacgggcata tgacatgtcg ccatcactgc
     8041 cagagtcatc agagaagtag tgcagacccg ctacttttcc gtacaaatcc agtttattgc
     8101 catctttatt atagacttca gccgcatttg ctgcgccagc aactaataac gccgggacca
     8161 gcattgccag aacttttctt ttcattatgt attcccttgt gattataatc ttcatgaata
     8221 tatcaataag tgccgttatc ca
//
LOCUS       DAZUCE010000218         8163 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZUCE010000218 DAZUCE010000000
VERSION     DAZUCE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.218"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            134..1117
                     /locus_tag="ACHY03_004173"
     CDS             134..1117
                     /locus_tag="ACHY03_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3907346.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKIVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            1166..2650
                     /locus_tag="ACHY03_004174"
     CDS             1166..2650
                     /locus_tag="ACHY03_004174"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFS3907347.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            2643..3614
                     /locus_tag="ACHY03_004175"
     CDS             2643..3614
                     /locus_tag="ACHY03_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3907348.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            3611..4567
                     /locus_tag="ACHY03_004176"
     CDS             3611..4567
                     /locus_tag="ACHY03_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3907349.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            4654..5703
                     /gene="yahK"
                     /locus_tag="ACHY03_004177"
     CDS             4654..5703
                     /gene="yahK"
                     /locus_tag="ACHY03_004177"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFS3907350.1"
                     /translation="MKINAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHALGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            5946..6761
                     /gene="yahL"
                     /locus_tag="ACHY03_004178"
     CDS             5946..6761
                     /gene="yahL"
                     /locus_tag="ACHY03_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFS3907351.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWITNRVQELGDRYYPVNKHVKIRYTL"
     gene            7174..7419
                     /gene="yahM"
                     /locus_tag="ACHY03_004179"
     CDS             7174..7419
                     /gene="yahM"
                     /locus_tag="ACHY03_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFS3907352.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(7436..8107)
                     /gene="yahN"
                     /locus_tag="ACHY03_004180"
     CDS             complement(7436..8107)
                     /gene="yahN"
                     /locus_tag="ACHY03_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFS3907353.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
BASE COUNT         2045 a         1793 c         2136 g         2189 t
ORIGIN      
        1 tataaccata tttttaatag ctgcctgaaa tagagctgtt tattctgcat aaaaatattc
       61 gaaaattctt attctctcca gcgcgctaat ttgggcgaac acttcctgac taccctgcaa
      121 tgaggctgaa atgatgaata aacgttttgt tatcaatatg gtgtcttcgc tgttattagg
      181 cgctgcgcta atttctgcgc cgctccaggc agcggaaaaa atcgtggtga atatttctaa
      241 agtcgatggt atgccgtggt ttaatcgcat gggcgaaggt gtggttgagg cgggtaaagc
      301 ctttggtgtt aatgcctcac aggtggggcc ttcaagtact gatgctccgc aacaagttaa
      361 aattattgag gatttaatcg cccgtaaggt taatgcaatt actatcgtgc caaacgatgc
      421 caacgtgctg gaaccggtat tcaaaaaagc gcgagatgcc ggaattgtgg ttctgaccaa
      481 cgaatccccc ggtcagccaa gtgctaactg ggatatcgaa atcattgata acgaaaaatt
      541 tgccgctgaa tatgttgagc atatggcgaa acgcatgggt ggcaagggcg gctatgtgat
      601 ttacgtgggt agtttgaccg tgccgcagca taacttatgg gctgacttgc tggtgaaata
      661 ccagaaagag cattacccgg atatgcatga agttacccgc cgcatgccgg ttgccgagag
      721 tgtggatgac tcacgtcgta ctacactgga cctgatgaaa acctatcctg acttgaaagc
      781 ggttgtctcc tttggttcaa acggtccgat tggcgcggga cgtgcggtga aagagaaacg
      841 ggcgaaaaat aaagtcgcag tctatggcat gatgatccct tcacaggcgg catcactgat
      901 taaaagtggt gatatcaccg aagggattac ttacgatccg gcctctgcag gttacgcact
      961 cgcagccgtc gccagcacgt tgctgaaggg tgaagagatc aagcctggat tagaaatgca
     1021 gaatctgggt aaggccgacg tagatatgga taagcgaatt atccgtttcc ataaggtatt
     1081 actggtcaac aaagacaata tcgactcact ttattaatcc atttcttcgg ggcgaaagcc
     1141 ccgattctta ttgccagggg atgttatgga aaccttcctt tcccttcgtc atatcaacaa
     1201 aacgtttcat gccacgcgag cgctgcgcga tgtttctctc gactttatgt cgggtgaagt
     1261 gcactgcctt gccgggcaaa acggctgtgg taaatcgacg ttgattaaaa ttatgtccgg
     1321 tgtttatcgc ccggatgagg gagcggaaat tacgcttggt gggaaaaact ggtcaaagct
     1381 gacacccgcc gcttcggtgg cgcaggggat tcaggtgatt tatcaggacc tctctttatt
     1441 tcctaacctg agtgtctggg agaatatcgc cgtgaatcac tatcaccacg gtctgtttgt
     1501 taaccgccgt cgtctgcgtg aggtggcgca ggcggcaatg actagtatta acgtcacatt
     1561 gccactggat acgctcgttt ctgagctttc aattgcccgc tgtcagctgg tggcaatttg
     1621 ccgtgcactg gcgcaggacg cacggttgat cgtgatggat gaaccaaccg cttcgctgac
     1681 ccatcaggaa gtgcaagggt tattacaggt ggtgcatcaa ttgcgtgaac gcgggatctg
     1741 cgtggtcttt gtcagtcatc gtctggaaga agtgatggaa gtttccgacc gtatttcagt
     1801 gctgaaagat ggtgagctgg tcgggacttt tccggctgca gagatgacca caaaacaact
     1861 cggtttcctg atgaccggcc aggagtttga atatcaggtg cgagagttgt ggcagggaaa
     1921 atccagcacc ccggtgctgg aggtgcgtaa tttaagtcgt catggggaat atctaaatat
     1981 taacttgcgg gtggaagcgg gcgaagtggt atcgattgtt ggcctgcttg gtgcggggcg
     2041 cacggaatta tgtctgagtc tgtttggtat gacccgaccg gatgctggcg agatcctcat
     2101 caacggccag ccggttacac tgcatagtaa ccaggacgcc atccgccacg gtattggtta
     2161 tgtttcggaa gatcgcatgt cgcgcggttt ggtgatggcg caatctattg aagacaacat
     2221 cattagcact gtttttcaca aggtaaaaga tcgcttcggc tttctgagtg aaactaaagt
     2281 gcgtgacctg gtggacaggc taatcaaggc attgaccatc aaagtctctg atcctcatct
     2341 cccggtgaat acgctttctg gtggtaacgc gcagcgagta tcgatcgcca aatggctggc
     2401 gattgggcca cgactgttga ttctcgatag cccgacagtc ggtgttgata tcgccaacaa
     2461 agcaggaata tacggcatta tcagcgatct ggcggctcat ggcattgcgg tattgatgat
     2521 ctgcgatgaa attgaagaag cgtggtatca aagccaccgg attttggtaa tgcaaaaagg
     2581 tcagatcacc catagcttcc tgcctgacag cagttctcag gccaggattg cggaggtggt
     2641 aaatggctga actgaaaaaa cgccatgaat tctggctggc attactgatt gtggtgctgt
     2701 ttgttggcct tgcctggcgc agcgacgagt ttctgacatt cggtaatttg tacgatctcg
     2761 ccaataacta tgccatgttg actattctcg cctgtggttt gtttgtggtg ttgatttccg
     2821 gtggaattga tatttcgttt ccagcaatga ccatcattgc gcaatacggc atggtgctgt
     2881 tgctgcaaaa aattggtggc aacttcgctg tcgcgtttgc actggcgggc tgcatcggca
     2941 ttttacttgg cttaattaac gccttactgg ttaatcgcct acgggtgcct tctatcatca
     3001 tcactatctc gacgctgaat attttctatg gcctgctgtt atggctgagt aaaggtgtgt
     3061 ggctgtacga ctttccgccg tggtttgagc agggggttat gttgttcaag tacaccgatg
     3121 ctgatggcta tgactatggc cttggtctgc cgctgatcgc catgattacg gtggtgctgc
     3181 taacagcgtt tatcatgaat ttcaccagtg tagggcgcaa aatttatgcc cttggcggga
     3241 accgcgaatc agccagtcgc atcggcttta gcgtgctgaa actgcaactt ttcgtctacg
     3301 gctatatggg attgatgtct ggcgctgcgg gtgtagtgca gtcgtggacg gtgatgactg
     3361 tcgcccccga ttctcttctg ggttatgagc tgacagtact ggctgcggtg gtgcttggcg
     3421 gcactagttt gctcggcggg cgcggcacgt taaccggtac tttgctcggc gtggtgttgt
     3481 tggcagtgat gcaaaacggg ctaaatttat tgggagtctc gtcttactgg caaacattga
     3541 tcaccggcat catcatcgtt gccagcatta gtgccacggc gtggagtcag catcagaacc
     3601 ggagtctgct atgaaaaaat cgtggcgtaa taacgttgag ttttacttga tcggcctgct
     3661 ggtgctaact gttgccgcgt tcagcattac tatgccggag attttctggt cgataagtaa
     3721 tttccagtcc gtcgcttcgc aaatgcccgt gctgggcatt ctggcgttgg ctatggcggt
     3781 gaccatgctt tgcggtggca ttaacttgtc aattatagcc actgctaacg cctgttcact
     3841 ggtgatggcg tgggtggcga ctcagtatcc accgggtatt gctacggttg tcgctacgtt
     3901 actggcgggc gctggcgcgg cggtgataat cggtttatgt aacggcgtat tgattgctgg
     3961 tattcgagtg tcgccaattc ttgcaacgct tgggatgatg acgttgctca aagggataaa
     4021 tattctggtg acaggtggta gcgccattgc caattatccc tcatgggtgc tgtggttgaa
     4081 tcatgcgcaa tggttcggta ttccgctacc gatgtggttg tttgccgcag tggcactggg
     4141 gctgtggata ttgctggaga aaacgccgct cggtaaagcc atttacctga ttgggtctaa
     4201 cgaacgagca acactttact ccggcattaa cactcgtcgg gtgctgattt gggtttatgt
     4261 catttccgcc ttgctttgtg cggtagcggc atttttgatg atgtcgaaac tcaactcggc
     4321 taaggcctct tatggcgaat cgtacttgct ggtgtcaatc ctcgcggcag tactcggcgg
     4381 ggttaatccg gacggcggca gcgggcggat aattggtatg gtgctggcgc tgtttcttct
     4441 gcaaattatt gaaagcggct tcaatattct cggtataagc ccctacctga cgatggcctt
     4501 atggggaacg ttgctgctct gtttcattca ggctcgcggc atgttggggc tggatcgggt
     4561 ggtttaaata ttggctatat tcaatggacg cgttttgccg cgatgacata tcaggcatcg
     4621 ccaaatacac atagctaatc aggagtaaac acaatgaaga tcaacgctgt tggtgcatat
     4681 tccgctaaac aaccacttga accgatggat atcacacggc gtgaaccggg accgaatgat
     4741 gtcaaaatcg aaatcgctta ctgtggcgtc tgccattccg atctccacca ggtccgttcc
     4801 gagtgggcgg ggacggttta cccctgcgtg ccgggtcatg aaattgtggg gcgtgtggta
     4861 gccgttggtg atcaggtaga aaaacatgcg ctgggcgatc tggtcggtgt cggctgcatt
     4921 gtcgacagtt gtaaacattg cgaagagtgt gaagacgggc tggaaaacta ctgtgatcac
     4981 atgaccggca cctataactc gccgacgccg gacgaaccgg gccatactct gggcggctac
     5041 tcacaacaga tcgtcgttca tgagcgatat gttctgcgta ttcgtcaccc gcaagagcag
     5101 ctggcggcgg tggctccttt gttgtgtgca gggatcacca cgtattcgcc gctacgtcac
     5161 tggcaggccg gaccgggtaa aaaagtgggc gtggtcggca tcggcggtct gggacatatg
     5221 gggattaagc tggcccacgc gatgggggca catgtggtgg catttaccac ttctgaggca
     5281 aaacgcgaag cggcaaaagc cctgggggcc gatgaagttg ttaactcacg caatgccgat
     5341 gagatggcgg ctcatctgaa gagtttcgat ttcattttga atacagtagc tgcgccacat
     5401 aatctcgacg attttaccac cttgctgaag cgtgatggca ccatgacgct ggttggtgcg
     5461 cctgcgacac cgcataaatc gccggaagtt ttcaacctga tcatgaaacg ccgtgcgata
     5521 gccggttcta tgattggcgg cattccagaa actcaggaga tgctcgattt ttgcgccgaa
     5581 catggcatcg tggctgatat agagatgatt cgggccgatc aaattaatga agcctatgag
     5641 cgaatgctgc gtggtgatgt gaaatatcgt tttgttatcg ataatcgcac actaacagac
     5701 tgaaaaaatt aataaatacc ctgtggttta acatattaac ttcgctctcc acttaacttt
     5761 ttagttaagg agagcgaaat aatatcaaag tagcagtaaa acctataacg taaattcaaa
     5821 ttgttaaatt aactccctcc agtacacaat acttcacacg ttagttatga gcgatttctg
     5881 atagtgcctg gtttaattag agctttatta tctgcgacgt ttatttttat ttaagagaga
     5941 tcgcgatgat atcattaaaa gctccgcaca ataatttgat gccatatacg caacaaagca
     6001 tccttaatac ggttaaaaac aatcagttac cagaggatat caaaagctcc ctggtttcct
     6061 gtgtcgatat attcaaggtt ttgattaaac aatattatga ttatccttat gattgtcgtg
     6121 atgatttagt cgatgacgac aaactcattc atctcatggc tgccgtacga gactgtgagt
     6181 ggtcagacga taacgcactc accataaatg tgcagtttaa tgatttccct ggattttatg
     6241 actggatgga ttaccctgat cacccggtta agtttgtttt tcgcatactt gaaaatcaaa
     6301 aagggacagt ttgggtatat gaccaggatg atgcttttct tgatattaaa gcaaatgtgc
     6361 aggctggacg ctttaccggg cttaagaaat tagtgcagtt tatcgattca gtgcgaacag
     6421 attgcaaatg tattcttctt gaacaccata tgcctttgct gagaattttc cctaaaggga
     6481 aagagtgtat gcatgtggaa aaatggcttc gcgaaatgtc gtctattccg gagacagacg
     6541 cgcccattaa gcaggcgctt gcgcatggat tattattaca cttaaaaaat atctaccctg
     6601 tttttcctga atcactggtg atgttattgc tctctgttct ggacgtaaaa acatacagag
     6661 atgatgcgcg attaaatcaa tggataacta atagagtgca ggaattaggt gacagatatt
     6721 atcctgtaaa taagcacgtt aaaatccgct acactcttta gttcagaaag ggttccacct
     6781 gcaagttgtt attccaccag agagtgaatc gcaatgcagg taatcatttc atctgtatat
     6841 ctgtatgcca actggcattc catctctttt ttcgccgata ttttgttgtg atcggcgaca
     6901 cttcgcttaa aaaaacacca gcagtggttt cgcagctatg cggtgtataa aaaatgatct
     6961 tatgcagatg ttttgtgaat gtgtgggttg acattcatat gaaaaaaatc ataattccat
     7021 catgtttgtg tggggtcttt tctgtacctt acgcatcgca ctcaagcctg acagaaaata
     7081 tgctgtaagg ctcatatcaa aaaccgccat tagctcatca ggaagagcag acgacaacca
     7141 ggattgttgt atggtacggg gttcgaggcc tcgatggcgg tccaactttt caaaactctt
     7201 ctgaatcaga tccccttatt atcatccctg caaagtggaa cattgccatt gtttggctat
     7261 tcaggacggg tcagaccaat gaaaaaagcc cacacagggg agagtgggct gaaatgggaa
     7321 gctaaagact caagtaaact tatcggaaat aaggaccacg cattacgggg gctatcatcg
     7381 ccgatggtcg ggattcggca aatcaggttg attacgtaga gagtaaatta tctgctcacc
     7441 gctgcgtcac cccttcgtaa atcaggcgta gcgcgaatac accaataatt gcaccaataa
     7501 cccgactggc aacgcgttgc atacgcccat aagcacgacg cacagcgggc aaagaaaacg
     7561 cctgactaag aaaaactcgc cagataattg atgcgagcac aatccccgcc caggccatta
     7621 aacgtgccca tgttggtgtt tcggcattta atgttactga gaaaatacta ataaaaaata
     7681 aaacggtttg tgggttagag agatcggtaa ttaatccacg gcgaaaaaag acataccagg
     7741 gggcgctaat cggttgttgt agtgtgctca tttgcggtgt tgactggcgg cgcatgctgc
     7801 accacgcaaa ccataagaga taagcgccgc cgacgattct gataagcgaa aaaatctcct
     7861 cacactgcgt aattagcgtt gcaagaccaa acaaacccaa cccggaataa aatgcatcgc
     7921 ccagcgccac gcccagcccg gtcagcaccc ctgcgcgtcg accggaagcc aggctggttt
     7981 gtactaccac aaagagattg gctcccggat taaaaaaagt aatcacgaac agtcctacgg
     8041 tcaggtaaac ggcgtgcaaa ggatccatag tgatttcatc cataaataag tgaagtaact
     8101 tcatcatatt tctaccaaaa ataatcgggt gcgagagaga tcacaaagtg tcttatttcc
     8161 ggt
//
LOCUS       DAZUCE010000219         8145 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZUCE010000219 DAZUCE010000000
VERSION     DAZUCE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.219"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            255..2492
                     /gene="nrfB"
                     /locus_tag="ACHY03_004181"
     CDS             255..2492
                     /gene="nrfB"
                     /locus_tag="ACHY03_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFS3907354.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDVLIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2479..5451
                     /gene="nfrA"
                     /locus_tag="ACHY03_004182"
     CDS             2479..5451
                     /gene="nfrA"
                     /locus_tag="ACHY03_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFS3907355.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEKRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGIGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5452..6342
                     /locus_tag="ACHY03_004183"
     CDS             5452..6342
                     /locus_tag="ACHY03_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFS3907356.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6525..7286
                     /gene="envY"
                     /locus_tag="ACHY03_004184"
     CDS             6525..7286
                     /gene="envY"
                     /locus_tag="ACHY03_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFS3907357.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(7332..7408)
                     /locus_tag="ACHY03_004185"
     tRNA            complement(7332..7408)
                     /locus_tag="ACHY03_004185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7372..7374),aa:Arg,seq:tct)
     gene            7651..>8145
                     /gene="fimZ"
                     /locus_tag="ACHY03_004186"
     CDS             7651..>8145
                     /gene="fimZ"
                     /locus_tag="ACHY03_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFS3907358.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGL"
BASE COUNT         2031 a         2109 c         2070 g         1935 t
ORIGIN      
        1 ctgctcacct ccaggcactt ccactacatc atgacctgat cggtgccacc actcctcttc
       61 actatgacgt taacctcccc tccttgacac cacgcttttg tctacattct acgcaaacgg
      121 caacttttat aaccagtgta aaaataacgt gccgcaatag tcatacctat taatggtaaa
      181 aagctgtcac aattcataaa aaaccttaat atacgccacc ctaaacataa ccagcgttaa
      241 tgtaaggttt ttgtgtggac tggcttcttg atgtttttgc tacctggctc tacggcttaa
      301 aagtaatcgc gataacgtta gcggtcatca tgttcatcag cgggctggac gattttttta
      361 ttgatgtcgt ctactgggta cgccgcatta aacgcaagtt gagtgtttat cgccgctacc
      421 cgcgaatgag ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg attatggttc
      481 cggcgtggaa tgaaacgggc gtcatcggca atatggccga gctggcggcg accacgctcg
      541 actacgaaaa ctatcatatc tttgttggca cctaccccaa cgacccggac actcagcgtg
      601 atgttgacga agtgtgcgct cgcttcccga atgtgcataa ggtagtctgc gcgcgtcctg
      661 gccccaccag caaagccgac tgtctgaaca acgtgctgga cgccatcacc cagtttgagc
      721 gtagcgccaa tttcgcattt gctggtttta ttctgcatga cgccgaagat gtgatttcac
      781 cgatggaatt gcgtctgttc aactatctgg tcgagcgtaa agatctgatt cagatcccgg
      841 tatacccatt cgaacgcgaa tggacgcact tcaccagcat gacttacatt gatgagtttt
      901 cagaactgca tggcaaagat gttccggtgc gtgaagccct cgccggacaa gtacccagcg
      961 caggcgtcgg cacctgtttc agccgccgcg ccgtgaccgc tctgttagct gatggtgacg
     1021 gtattgcttt cgacgtgcaa agtcttactg aagattacga cattggcttc cgcctgaaag
     1081 aaaaaggtat gacggaaatt tttgtccgtt ttccggtggt ggatgaagcc aaagaacgcg
     1141 agcagcgtaa atttttacag cacgcacgga cgtcaaacat gatctgcgtg cgcgaatatt
     1201 tccccgatac cttttcgact gcggttcgac aaaaatcccg ctggatcatc ggcattgttt
     1261 tccagggctt taaaacccat aaatggacct ccagcctgac gctgaactac tttctctggc
     1321 gcgaccgcaa aggggcaatc agtaactttg tcagcttcct cgcgatgctg gtgatgatcc
     1381 agcttttgct gttgctggcg tatgaaagtt tgtggcccga tgcctggcat ttcctttcta
     1441 tttttagcgg cagcgcatgg ttaatgaccc tgctgtggct aaactttggc ttgatggtta
     1501 accgcatcgt gcagcgggtg attttcgtca ctggctacta cggcctcacg caggggctac
     1561 tatctgtcct gcgtcttttc tggggcaacc tgattaactt catggccaac tggcgcgcgt
     1621 taaaacaggt acttcaacac ggcgatccac gtcgcgtcgc gtgggataaa acaacgcatg
     1681 acttccccag cgtcactggc gatacccgct cgttgcgccc gttaggtcaa attctgctgg
     1741 aaaatcaggt catcactgaa gaacagctcg atacagcact gcgtaatcgc gtcgaaggtc
     1801 tacgcctggg cggttcaatg ctgatgcagg ggctgattag cgccgagcag ctggcacagg
     1861 cgctggcaga gcaaaacggc gtggcgtggg aatccatcga tgcctggcag atcccgtcct
     1921 cgctgattgc cgaaatgcca gcctccgtgg cgctgcatta tgcagtgctg ccgctgcgtc
     1981 tggaaaatga tgtgttaatt gtcggcagtg aagatggtat tgacccggtt tcgctggcgg
     2041 ccctgacgcg taaagtcgga cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg
     2101 tcacggggtt acgtcactgg tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt
     2161 acaatgcggt tcagcatcag tggctcacgg aacagcaggc cggtgaaatc tggcggcaat
     2221 atgtgccgca tcagttcctg ttcgccgaaa tactgaccac gctcggtcat attaatcgtt
     2281 cagcaattaa cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt
     2341 tggtcaccga aggcgttatc agccaggaaa cgttggatcg cgtcctgaca attcaacgcg
     2401 aattacaagt ttcgatgcaa tcactattac tcaaagcagg tttaaacaca gaacaggttg
     2461 cgcaactgga gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg atggtctggt
     2521 ttactgctga cgtctttatt gagtaccagc gcactcgcag acaatatcgg caccagcgca
     2581 gaagagctgg ggctgagcga ttatcgccat tttgttattt atccccgtct cgataaggcg
     2641 ctgaaggcac agaaaaataa cgacgaagca accgccatcc gcgaatttga atatatacac
     2701 cagcaggtgc cggataatat tccgctgact ttataccttg cggaagccta tcgccatttt
     2761 ggtcatgatg accgggcgcg gctgttgctt gaggatcaac tgaaacgtca cccaggagat
     2821 gcccgacttg agcgcagtct ggcggctatt ccagttgaag tgaaaagcgt tacgacagtt
     2881 gaagaactgc ttgcccagca aaaagcgtgc gatgctgcgc cgaccctgcg ttgtcgcagt
     2941 gaagtcgggc agaatgccct gcggctggca cagttacctg tcgccagagc gcaactgaac
     3001 gatgcgacgt ttgctgcatc gccggaagga aaaacgctgc gaaccgatct gctgcaacgg
     3061 gcaatctacc tgaaacaatg gtcccaggca gatacgctat acaatgaagc acgccaacag
     3121 aacacattaa gcgcggcaga acgccgtcag tggtttgacg tgcttcttgc cgggcagctg
     3181 gacgatcgga tcctggcgct gcaatcacag gggatcttca ccgatccgca gtcatatatt
     3241 acttacgcga ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca ttatctcatt
     3301 gaaaataagc cgctgtttac cacagacgca caagagaaaa gttggctcta tctgttatct
     3361 aaatacagcg ccaaccccgt tcaggcattg gcgaattata cggtgcagtt tgccgataac
     3421 cgccagtatg ttgttggcgc gacgctaccg gtgctgttaa aagaaggtca gtacgacgca
     3481 gcgcaaaaac tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg ttatgctgtc
     3541 agcgtggcga cccgtaacaa ggctgaagct ctgcgtctgg cacgcttgct gtatcagcaa
     3601 gaaccggcaa atcttacccg cctggatcaa ctaacctggc aactgatgca gaacgagcag
     3661 tcacgggaag ctgccgattt attgctgcaa cgctatcctt tccagggcga tgcgcgtatc
     3721 agccagactt taatggcgcg actggcgtct ctgctggaaa gtcatcctta cctggcaacg
     3781 ccggcgaagg tggcgatttt atcgaaaccc ttaccgctgg cggcgcaacg tcagtggcaa
     3841 agtcagttgc cgggtattgc agataattgc ccggcaatag ttcgcttgct gggcgatatg
     3901 tcgccttcct acgatgccgc cgcctggaac cgtctggcaa agtgttatcg ggacacgcta
     3961 cccggtgtgg cgttgtatgc atggcttcag gccgaacaac gacaacctag cgcctggcaa
     4021 catcgtgcgg tagcctatca ggcgtatcag gttgaggact acgccaccgc actggcggcc
     4081 tggcagaaaa tcagtcttca cgacatgagc aatgaggatc tgcttgctgc tgccaatacc
     4141 gcccaggcgg caggaaatgg tgcggctcgc gatcgctggc tacaacaggc agaaaaacgt
     4201 ggactgggaa gcaatgcgct ctactggtgg ctgcatgcgc aacgttacat tcctggtcag
     4261 ccggaactcg cactgaacga tctcacgcgc tcaatcaata ttgcgccttc tgccaacgct
     4321 tacgttgcgc gggcgacaat ttatcgccaa cgtcataatg tcccggccgc ggtgagtgat
     4381 ttgcgcgccg cgctggaact ggaaccgaat aatagcaaca cccaggcagc gcttggttac
     4441 gccttgtggg atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa
     4501 gggctaccgg acgatccggc actgatccga caactggcct atgtgaacca gcgtctggat
     4561 gacatgcctg cgacgcagca ctacgcccgg ctggtgattg atgacattga taatcaggcg
     4621 ctgataaccc cactgacccc agagcaaaat cagcaacgct tcaatttccg ccgtctgcat
     4681 gaggaggtcg gtcgccgctg gacgttcagt ttcgattctt ccatcggctt gcgttccggc
     4741 gcaatgagta ccgctaacaa taatgttggc ggcgcagcgc cagggaaaag ctatcgtagc
     4801 tacgggcaac tggaagccga ataccgcatc ggacgcaata tgctgctgga aggcgacctg
     4861 ctttcagttt atagccgcgt ctttgccgat accggagaaa acggggtgat gatgccggtg
     4921 aaaaatccga tgtccggcat cggtctgcgc tggaagccgc tgcgcgatca gatctttttc
     4981 ctcgccgtcg aacagcagtt gccgctgaac gggcaaaatg gcgcatccga taccatgctg
     5041 cgcgccagcg cctcattctt taatggcggc aaatacagcg acgaatggca cccgaacggt
     5101 tcaggctggt ttgcccaaaa cctgtacctc gatgcggcgc aatatatccg ccaggatatt
     5161 caggcgtgga cggcagatta tcgcgtcagc tggcatcaga aggtagctaa cggacagact
     5221 attgagcctt acgctcacgt tcaggacaac ggctatcgtg ataaaggcac tcagggcgcg
     5281 cagcttggcg gtgtcggggt ccgctggaat atttggaccg gcgagacgca ctacgacgcc
     5341 tggccgcaca aagtcagtct cggcgtcgag tatcaacata cctttaaggc gattaatcaa
     5401 cgtaacggag agcgcaacaa cgcgtttctc accattggag tgcactggta aatgcgtaag
     5461 ttcattttcg tattgctgac actgcttttg gtcagccctt tttcctttgc gatgaaaggt
     5521 attatctggc aaccacaaaa ccgagatagt caggttaccg atacccagtg gcaggggctg
     5581 atgagtcagt tacgtttgca aggcttcgat accctggttt tgcaatggac ccgttacggc
     5641 gatgcattta cccagccaga acagcgcacg ttattgttta agcgggccgc agctgcgcaa
     5701 caggctggcc tgaagctgat tgtcgggctg aacgccgatc cggaattttt tatgcaccag
     5761 aaacagtcgt ctgcagcgct ggaaagctat cttaaccgcc tgctggctgc cgatctccag
     5821 caagccagat tatggagtgc cgtacctggc gtaacgccgg atggctggta catcagcgcg
     5881 gaaattgacg acctgaactg gcgcagcgaa gccgcccgtc aacctttgct aacatggtta
     5941 aacaacgcgc agcggctgat tagcgatgtt tcagcaaaac cggtttatat cagtagtttt
     6001 ttcgccggaa acatgtcgcc agatggctat cgccaactgc tggaacacgt aaaagcaacc
     6061 ggcgttaatg tctgggtaca ggatggcagc ggcgtggata aacttaccgc tgaacagcgt
     6121 gaacgttatc tacaggccag cgccgattgc caaagctccg ccccagcaag cggcattgtt
     6181 tatgaactgt ttgtcgccgg caaaggcaaa acctttacag cgaaaccgaa accggacgca
     6241 gaaattgcct cgctgttagc gaaacgttcc tcttgcggta aagacactct ctatttctct
     6301 ctgcgctatt tgcccgtcgc gcacggcatt ctcgagtatt aaatctcctc caggtaagtc
     6361 gggtacgacc tggcttacct ctttcgctct cgtcagaatt ccatcaagat taattgctaa
     6421 aaagcgtgct aaagaaagaa gatcgttatc ggcgaacggg ccgatcactc atgcaggaag
     6481 catagatcct ttgagcggag tttctgatat ctgaggagtg cgaaatgcaa ttgagcagca
     6541 gtgaaccttg cgtggtgatc ctgaccgaaa aagaggtaga ggtaagcgtc aataaccatg
     6601 ctacgtttac ccttccgaaa aactacctgg ccgccttcgc gtgcaacaat aacgtcattg
     6661 aactctcaac gttaaatcac gtattaatca cccacatcaa ccgtaacatc atcaacgatt
     6721 atctgttgtt tttaaataag aacttaacct gtgtaaagcc ctggtcgcgg ctggctaccc
     6781 cggttatcgc ttgtcatagc cgtacaccgg aagtgttccg gctagccgcc aaccacagca
     6841 agcagcaacc cagcaaaccc tgcgaagcgg agttgacgcg cgcattgctt tttaccgtat
     6901 tgtctaactt tcttgagcaa tcgcggttta ttgccctact gatgtatatc ttacgcagca
     6961 gcgtccgcga cagcgtctgc cgcattattc aaagcgatat tcagcattac tggaatctgc
     7021 gaattgtcgc cagttcgcta tgtttaagcc ccagcctgct caaaaagaaa ttaaaaaacg
     7081 aaaataccag ctatagccag attgtcacag agtgtcgtat gcgttacgcc gtacagatgt
     7141 tattgatgga taacaaaaat atcactcagg tggcgcaatt atgtggctat agcagcacgt
     7201 catactttat ctctgttttt aaggcgtttt acggcctgac accgttgaat tatctcgcca
     7261 aacagcgaca aaaagtgatg tggtgaaggg caaagcggaa acggataaga cgggcataaa
     7321 tgaagaagaa atggcgcgcc ctgcaggatt cgaacctgcg gcccacgact tagaaggtcg
     7381 ttgctctatc caactgagct aagggcgcgt tgataccgca atgcggtgta atcgcgtgaa
     7441 ttatacggtc aacccttgct gagtcaatgg cttttgatct ggttgctgaa caagtgaacg
     7501 accgcgtctg attttctgat ttatttcgct atagcggcaa acaaacgcac accgctgcgc
     7561 gtctgaatca agaaaacccg tattttcatg tatcaaagta caatttaccg ccctaacgga
     7621 aaattgtccg ctcctatgag actggtaact atgaaaccaa cgtcggtgat cattatggat
     7681 actcatccta tcatcagaat gtctattgaa gttctgttgc aaaaaaacag tgaattgcag
     7741 attgtcctga aaacggatga ttaccgcata accatcgatt atctccgaac ccgtcctgtt
     7801 gatttaatca ttatggatat agacttgccc ggaacagacg gttttacctt cctgaaaagg
     7861 atcaaacaaa tccagagcac agtgaaagtg ttatttttat catcgaaatc agaatgcttt
     7921 tatgctggca gagcgataca agctggtgct aacggttttg tcagtaaatg caatgatcag
     7981 aatgatattt ttcatgccgt tcagatgatc ctctccggat acactttttt tcccagcgaa
     8041 acgctcaact atataaaaag caataaatgt agtacgaata gttcaacgat cactgtgcta
     8101 tctaatcgtg aagtgaccat attacgttat ctggttagcg gatta
//
LOCUS       DAZUCE010000220         8127 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZUCE010000220 DAZUCE010000000
VERSION     DAZUCE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.220"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(253..2139)
                     /gene="prpE"
                     /locus_tag="ACHY03_004187"
     CDS             complement(253..2139)
                     /gene="prpE"
                     /locus_tag="ACHY03_004187"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFS3907359.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(2179..3630)
                     /gene="prpD"
                     /locus_tag="ACHY03_004188"
     CDS             complement(2179..3630)
                     /gene="prpD"
                     /locus_tag="ACHY03_004188"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFS3907360.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDTLREKINCFEDPSFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(3664..4833)
                     /gene="prpC"
                     /locus_tag="ACHY03_004189"
     CDS             complement(3664..4833)
                     /gene="prpC"
                     /locus_tag="ACHY03_004189"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFS3907361.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTDSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(4989..5879)
                     /gene="prpB"
                     /locus_tag="ACHY03_004190"
     CDS             complement(4989..5879)
                     /gene="prpB"
                     /locus_tag="ACHY03_004190"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFS3907362.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQAK"
     gene            6118..7704
                     /gene="prpR"
                     /locus_tag="ACHY03_004191"
     CDS             6118..7704
                     /gene="prpR"
                     /locus_tag="ACHY03_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFS3907363.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALSKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYALSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLETELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLRYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(7805..8080)
                     /gene="yahO"
                     /locus_tag="ACHY03_004192"
     CDS             complement(7805..8080)
                     /gene="yahO"
                     /locus_tag="ACHY03_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFS3907364.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTADIYKKK"
BASE COUNT         1795 a         2293 c         2177 g         1862 t
ORIGIN      
        1 cctgcgtcgc atcaggcatc ggcgcatggt gtcgaatgcg gcgcgagcgc cttatccgac
       61 ctacggttca ggcctgtagg catgataaga cgcgcctgcg tcgcatcagg cactgagcac
      121 ggtgtcggat gcggtgcgag cgccttatcc gacctacggt tcaggcctgt aggcatgata
      181 agacgcgcca gcgtcgcatc aggcatctgc gcacaactgt cggatgcgcc acgcgacgca
      241 tccgacaata ccttactctt ccatcgcctg gcggatctga tccaacgacg caggatcatc
      301 aatggtcgtc agatccccag gatcgcgtcc ttcgcaaatc gcctggatcg tgcggcgcag
      361 catttttccg gatcgcgttt ttggcaattg cgagacaaac cagacgtgcg ccgggcggcc
      421 aaagttgcca atctggctgt ccaccagcgc cataatcgcc ttctcttgcg aatgcgccac
      481 atcacgatct tccagactgt cgctctcttt cggaatgaca aacgccaccg ccacctgccc
      541 tttcagcgca tctttcaccc caaccaccgc cacttcggca acgcccggat gactggaaat
      601 actctcttca atctcgcgcg tccccagccg atgcccggca acgttaatca catcgtcagt
      661 gcgcccgaga ataaagtgat aaccgtcagc gtcacggatg ccccagtcaa aggtggcgta
      721 caccgggcgg gaaaacagcg accagtacgt cttcacaaag cggtcgtcgt cgccccagat
      781 ggtctgaata caccccggcg gcagcggccc ttccaccacc agcatccctt tctcgttaac
      841 gccacacggt tcgccggtga cttcattaag caactgcacg ttatagccat acatcggcac
      901 gccggggctt cccagacgcg tcggcctgtc gtccagaccg cgagcaatcg ccataatcgg
      961 ccagccggat tcggtctgcc agtagttgtc gatgaccggc acatccagcg tattgctcac
     1021 ccaactggcg gtcggctcgt ccagcggctc ccccgccaga tagagaactt ccagcgacga
     1081 aagatcgtgt ttgcgaattt cagcggtagg gaattttttc agcacgcgaa tggcggtcgg
     1141 cgccgagaac atccggctca cctgatattt ctcgacgatt ttccaccaca cgccgcagtc
     1201 cggccaggtc ggtaatcctt cgtaaacgat agtcgccatc cccgccagca gcggcgcgta
     1261 gacgatatac gaatgcccca ccacccagcc gatatccgaa gcacaaaaga acacaccgcc
     1321 cgctttgccg ccaaaaatgg tgtccatcga ggtcgccagc gccaccgcat atccgccgac
     1381 gtcacgctgc acgcctttag gtttgccggt tgtgccggag gtgtagagaa tgcaggaggt
     1441 ttcgttggat tccagccacg ccaccggtac ccgcgcgccg atgtgttgat ggcgcaacga
     1501 cgcgaaatcg acatcccgcc cgctgacgcg cgccattttc gccagcccgc gatccaccag
     1561 caaaacatgg cgcggctggt gctgcgcctg acttatcgca tcgtcgagca attttttata
     1621 gggaatgatt ttgccaccgc gcgctccggc atcagccgag acaatcagca ccggtttagc
     1681 gtcatcaatt cgcgccgcca cactgtgcga ggcgaatccc ccaaacacca ccgagtgaat
     1741 agcgccaatg cgcgcgcagg ccagcagggt aatatgcgct tcggcaatca tcggcatata
     1801 caccagcacc cgatcgccac gctgcacgcc cagtgagcgc agcattgacg ccaccgcgtt
     1861 cacttcgtca tgcagctggc gaaaggtaaa ggtgcgctct tcttctgttt ccgaagagac
     1921 ggcaatcagc gccagcgcct ctggctgttt ctccagccag cggtcgatag cgttgtgaca
     1981 caagttggtt cggccttcac aaaaccaacg agcaaacgga ggattgctgt gatcgagcgt
     2041 ttgcgtaaag ggcgtctgcc agtcaatacg ccgggcctgc tcggcccaga actgctccgg
     2101 ttcgttaatc gaacgctgat aaaattcgct aaaagacata atgctctcct gttgaactta
     2161 cgccttaccg ctgtttgctt aaatgacgta caggtcgaga tactcattga ccggcatctg
     2221 ttccaggcga gttctgtcga gagaaacctc cagaatgcgc tgctgttggc gagtcgggaa
     2281 ctggcgcgcg agattgattt tgaatttatc gaccagtttc ggaataccat cctgacggcg
     2341 gcgagcatga ccaatggggt actccaccac cacttcttca aagcgcgtgc cgtcggtgaa
     2401 ctcaagggtt atggcattgg caatagcgcg tttttccggg tcgtggtagt cagcggtaaa
     2461 tgacggatct tcaaagcaat tgatcttctc gcgcagggtg tcgatgcgtt tatcttgcgc
     2521 aacgttgtct tcgtaatctg ccgccgttaa gcgcccgaac agcagtggga tcgccaccat
     2581 gtactgaatg cagtggtcgc ggtctgccgg gttattgagc ggcccctttt tgtcgatgat
     2641 gcgaatacag gcttcgtggg tgcgaatggt cactttttcg atatccgccg ccgttttgcc
     2701 tgctgcctgc atctgttcat agagcgtcat cgctgcttca actgccgtct gggagtggaa
     2761 ctccgccggg aaggagattt tgaacagcac attttccatc acgtaagaac cgtacggacg
     2821 ctggaagcgg aacgactcac ctttaaagga gacgtcgtag aagccccaca ccggcgcagt
     2881 cagggctgac gggtaaccca tttcgcccgt tttcgccatc agtgccagac gtaccgcgcg
     2941 ggaagtggca tcgcccgccg cccaggattt acgcgtaccg gtgttcggcg catggcgata
     3001 ggtgcgcagc gactgaccgt ccacccacgc cagcgaaaca gcgttgagaa tttcctcgcg
     3061 ggtcaggccg agcatttcgg cgaccacggc ggtggaagcc actttcacta acagaacgtg
     3121 gtcgaggccg acgcggttaa aggagttttc cagcgcgatg cagccctgaa tttcatgggc
     3181 tttgatcatt gcggtcagca cctgtttcat ggtcaacggc gctttgccgc tggcgaccgc
     3241 gttgcgcgaa agccagtctg ccgttgccag aatgccgcca aggttgtcgg aaggatgccc
     3301 ccactccgcc gccagccagg tatcgttgaa atcgagccaa cggatcatcg cgccgatgtt
     3361 aaatgccgcc tggacggggt cgagctggaa ctgagttccg gggacgcgca cgccgttagg
     3421 tacgacggtg ccggggacaa ttggccccag cagtttttta caggctgggt attcgagagc
     3481 ttcaagaccg cagccgagcg tgtcgagcag gcagtaatgt gcggtgtcgt aggctacttt
     3541 ggagctgatt tcgtagttca tgacgtaatc gacgatatca acgatttcac gatcaaattc
     3601 cgggcggatg ttgttgattt gagctgacat tgggtacgtt tccttattgt tattcgtaga
     3661 ggtttactgg cgcttatcca gcgcgacaaa ctggcggtct tccggtccaa cataattggc
     3721 ggaaggacgg ataattttgt tgtcctgacg ttgttcgata atgtgcgccg cccatccggt
     3781 gacgcgggcg ataacaaaca gtggtgtgaa catctcggtg ggaacgccca tcatgttgta
     3841 ggaaacagcg gagaaccagt cgagattggg gaacatcttt ttgctctccc acatcaccgt
     3901 ttccagacga tcggcgatgt tgtacatctt cagcgagccg ccttcctgcg agagctgctt
     3961 cgccacacgt ttaatcacct ggtggcgcgg gtcggcgatg gtataaaccg gatgcccaaa
     4021 accaatgacc acttctttgt tttccacgcg cttgcggata tctgcttcgg cttcgtctgg
     4081 cgtttcgtag cgttgctgga tctccagcga cacttcattc gccccgccgt gtttcggccc
     4141 gcgcagtgcg ccaatcgcgc caataatcgc ggaatacata tcagagtcag tacccgcaat
     4201 cacccgactg gtaaaggtgg aggcgttaaa ctcgtgttcg gcgtacagca ccagcgaaat
     4261 atgcatcgcc ttttcccagc tttgcgacgg cttttcgccg tgcagcagat gcaggaagtg
     4321 accgccgatg gagtcgtcat cggtttccgg ctggatgcgt tcgccgttat ggctgtagtg
     4381 ataccaataa aggagaatcg agctaagtga cgccagcagt ttgtcggcaa tatcccgcgc
     4441 gccagagacg gtatgcccct ctttttctgg cagcgtacag ccgagcgcgg aaacgccggt
     4501 gcgcataaca tccatcgggt gcgacgccgc cggtaaggct tccagcacag tacgcacatt
     4561 agccggtaaa ccgcgcaggg ctttcagttt cgttttgtaa gcggcgagtt cgtcacgggt
     4621 cggcagtttg ccgtggatca gcagatgcgc cacttcttca aattcgcaat gttccgccag
     4681 atcaagaata tcgtagccac ggtaatgcaa gtcattgcca cttttaccca cggtgcagag
     4741 cgccgtattg cccgccggaa cgccagaaag cgccacagat tttttcggtt taatgacatg
     4801 ggtactgttt tgcaggatcg ttgtgtcgct catattgtcc tcgtcattgt tatcatttgt
     4861 agggtcgaga ttgttgtcgg atgcgacgcc atgcgtctta tccgacctac agttcacccc
     4921 gtaggcctga taagtcgcgt cagcggcgca tcaggcatcg gttgtcaaat acaaccaacg
     4981 ggcgtttttt atttcgcctg gctacgggca aacaggtcgt cgagcttctc ttcgtactgg
     5041 tagtagttga tgctttcgta cagctcgttg cgggtctgca tggtgtcgat gacgcttttc
     5101 tgcgtgcctt cctggcgcag gacgttgtag acatgttcgg cggcgcggtt catggcgcga
     5161 aacgctgaaa gcgggtacag cgccatcgcg acatgagcgc tgcgtaattc gtcggtggta
     5221 aacagcggcg tggcgccaaa ttcggtaatg ttggcgagga ttggcacctg caccgcatcg
     5281 gcaaactggc ggtacatggc gagttcggta atcgcctccg ggaacaacat ctcggcaccc
     5341 gcttcaacat aggcctgcgc acgctcgatc gccgcatcca gcccctctac cgccagcgca
     5401 tcggtgcgcg ccatgatcac aaaatcagga tcggttttcg catccaccgc cgcgcggatc
     5461 cgatccacca tctcctcttt cgagacgatc gctttattcg gacgatgacc gcagcgtttc
     5521 gcaccaacct gatcttcaat atgcaatcct gccgcaccag ctttaatcat cgatttcacg
     5581 gtgcgcgcca cgttaaaggc cgaagaacca aaaccgatat ccgcatccac cagcagcggc
     5641 agcgaacaaa cgtcggtgat acggcgaatg tcggtcagca catcatcaag ggtagaaata
     5701 ccgagatcgg gcagccccag cgaccctgcc gccacgccac cgccggagag ataaattgcc
     5761 tgatatccgg cacgctgcgc caacagcgca tggttggcgt taatggtgcc aacaatctgc
     5821 aatggatttt ctttagacag tgcagcgcga aacgctttac ctggagagtg tagagacata
     5881 gcccatcctt tgttatcaac ttgttatttg cgttgataaa cacaaagcaa ggggtgtgcc
     5941 acaattgcct taataaaatt aaatctttaa tttcaatttg ttagagataa agtcatgaac
     6001 taatccgcaa atatgcgttt cagttaacgt ttcaggcaat gtttcaccgc gtttcattgc
     6061 aacaattatg aaacaaggct aaacccaata ttcggtttct taactttgcg gtgcgctatg
     6121 gcacatccac cacggctgaa tgacgacaaa ccggttatct ggacggtatc tgtaacgcgc
     6181 ctgttcgagc tgtttcgcga tatcagcctc gagtttgatc acctggcgaa cattacccct
     6241 attcagcttg gctttgaaaa agcagtgacc tacatccgca agaaactggc aaacgaacgc
     6301 tgtgacgcca tcatcgctgc tggctctaac ggcgcgtacc tgaaaagccg cctgtcagtg
     6361 ccagttattt tgattaaacc aagcggctac gatgtgttac aggcactgtc aaaagccgga
     6421 aaactcacct cttctatcgg cgttgtcact tatcaggaaa ctattccggc actggtggcg
     6481 tttcaaaaaa cctttaattt gcgcctcgat caacgtagct acattaccga agaagacgcc
     6541 cgcgggcaga ttaacgagct caaagctaac ggcaccgaag cggtggtcgg tgcggggctg
     6601 attaccgatc tggcagaaga agccggaatg accggaattt ttatctattc cgccgccacc
     6661 gtgcgccagg cgttcagcga tgcgctggat atgacgcgca tgtcgttacg ccataacact
     6721 cacgatgcca cccgcaacgc cctgagaact cgttacgtgc tgggcgatat gctcggtcaa
     6781 tcaccacaga tggaacaagt acggcagact attttgctgt atgccctctc cagtgcagcg
     6841 gtgttgattg agggggaaac ggggacgggc aaagagctgg cggcccaggc aattcatcgg
     6901 gaatattttg cccgccacga tgcgcgacag ggcaaaaagt cgcatccgtt tgttgccgtc
     6961 aactgcgggg cgattgccga atcgctgctg gaaacggagc tgtttggcta tgaggaaggg
     7021 gcgtttaccg gctcgcgacg cggaggtcgt gccgggctgt ttgaaatcgc ccacggtggc
     7081 acgctgtttc tggatgagat tggcgaaatg ccgctacctt tgcagacccg gctgctgcgg
     7141 gtgctggaag aaaaagaggt cacccgcgtc ggcgggcatc agcctgttcc ggtagatgta
     7201 cgggtcatta gcgccactca ctgcaatctg gaagaagata tgcggcaagg gcagtttcgc
     7261 cgtgatctgt tttatcggct gagtattttg cgtctgcaat tgccaccact gcgcgagcgg
     7321 gtggcagata ttctgccact ggcggaaagt tttttgaaag tgtctctggc ggcgctctcc
     7381 gccccatttt ctgctgcatt acgccagggg ttacaggcaa gtgaaactgt gctgctgcgc
     7441 tacgactggc cgggcaatat tcgagaactg cgcaatatga tggagcgact ggcgctattt
     7501 ttaagtgtgg aaccgacgcc ggatttaacg ccgcaatttt tgcagctgct actgccggaa
     7561 ctggcgcgcg agtcggcgaa aactcccgct ccacgcttac tgacaccaca acaggcactg
     7621 gagaaattta atggcgataa aacagcagcg gcgaattatt taggcatcag tcggacgacg
     7681 ttctggcggc ggctgaaaag ctgaactgcc gaatgcagcg cgaacgcttt atccggcata
     7741 aatttcaata gatgatgatc accgataaac ttacgcatcg gggatgttta catagggttc
     7801 agaattactt cttcttataa atatccgccg tgccgtggat cttattgtca gtttgaccag
     7861 aggtcagcac caacacatca gccccttttt catcggcttt tttgattaaa tcttcttttg
     7921 catctgcagt cgacatttca ttactggttg aaatatcacc tattttttca tactgcgatt
     7981 caactttttc aaattccgct ttggtcatca attcagcggc atagacattg gtaacggcaa
     8041 acgctaacgc accgactaac attttagaga ttattttcat attacggctc cagatttgtt
     8101 atcacactat cacccaccca cattatt
//
LOCUS       DAZUCE010000221         8111 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZUCE010000221 DAZUCE010000000
VERSION     DAZUCE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.221"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..100
                     /locus_tag="ACHY03_004193"
     CDS             <1..100
                     /locus_tag="ACHY03_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907365.1"
                     /translation="IHKNKMIEHYVIATSNDVIDVLSTVSPQIVYD"
     gene            216..1016
                     /gene="ydcF"
                     /locus_tag="ACHY03_004194"
     CDS             216..1016
                     /gene="ydcF"
                     /locus_tag="ACHY03_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFS3907366.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            1213..2652
                     /gene="aldA"
                     /locus_tag="ACHY03_004195"
     CDS             1213..2652
                     /gene="aldA"
                     /locus_tag="ACHY03_004195"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFS3907367.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2694..3695)
                     /gene="gap"
                     /locus_tag="ACHY03_004196"
     CDS             complement(2694..3695)
                     /gene="gap"
                     /locus_tag="ACHY03_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFS3907368.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVNGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3884..4414
                     /gene="cybB"
                     /locus_tag="ACHY03_004197"
     CDS             3884..4414
                     /gene="cybB"
                     /locus_tag="ACHY03_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFS3907369.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFLPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4659..4832
                     /locus_tag="ACHY03_004198"
     CDS             4659..4832
                     /locus_tag="ACHY03_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907370.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            4858..4926
                     /locus_tag="ACHY03_004199"
     CDS             4858..4926
                     /locus_tag="ACHY03_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907371.1"
                     /translation="MNLLVRCAGKIPALALTWTCKP"
     gene            complement(4904..5053)
                     /gene="hokB"
                     /locus_tag="ACHY03_004200"
     CDS             complement(4904..5053)
                     /gene="hokB"
                     /locus_tag="ACHY03_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFS3907372.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            5443..7083
                     /gene="trg"
                     /locus_tag="ACHY03_004201"
     CDS             5443..7083
                     /gene="trg"
                     /locus_tag="ACHY03_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFS3907373.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRVGGMFMIGAFVLALVMTLIT
                     FMVLRQIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSNEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEQVSFATV"
     gene            complement(7121..8044)
                     /gene="ydcI"
                     /locus_tag="ACHY03_004202"
     CDS             complement(7121..8044)
                     /gene="ydcI"
                     /locus_tag="ACHY03_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFS3907374.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
BASE COUNT         2107 a         1881 c         2053 g         2070 t
ORIGIN      
        1 aattcataaa aataaaatga ttgaacatta tgttattgcc acgtcaaatg atgttattga
       61 tgttttatca acagtttctc cgcaaatagt gtacgactaa aaccagtgtc tggttcgcga
      121 tggcgagata gaaaaagcat tatcctcctt tcagagtgct ccgcttctca ctattatctc
      181 acgcagtatt cttaagggaa cgataaggag gaaccatgaa cattaccccg tttccgacgc
      241 tttcgacggc aactatagat gccataaatg ttatcggaca gtggctggcg caggatgatt
      301 tctccggtga ggtgccgtat caggctgatt gcgtcatact tgcaggaaat gcggttatgc
      361 cgactatcga tgcggcatgt aagattgccc gcgatcagca aattccttta ctgattagtg
      421 gtggtatcgg tcactcgaca acttttttgt atagcgccat cgcacagcat ccgcactaca
      481 acactatccg caccactggc agagcagaag cgaccatcct ggcggatatc gctcatcagt
      541 tctggcatat tccgcatgaa aaaatctgga ttgaagacca gtcaacaaac tgcggtgaaa
      601 acgcacgctt tagcatcgcg ctattgaatc aggccgtaga acgagttcac acagctatcg
      661 ttgtacagga ccccaccatg cagcggcgca cgatggcgac gttccgccgt atgaccgggg
      721 acaatcccga tgcaccacgc tggttaagtt atcccggatt cgttcctcag ttaggaaata
      781 acgcagacag tgtaatcttt attaatcagt tacaaggatt atggccggtt gagcgttatc
      841 tctcactact cactggcgag ctgccgcgtt tacacgatga tagcgatggc tacggtcccc
      901 gcgggcgaga ttttatcgtt cacgttgatt ttccggcaga agtcattcag gcatggcaaa
      961 cgcttaaaca cgatgcggtg ctcatcgagg cgatggaaag tcgctcgtta cgttaaaaat
     1021 tgcccgtttg tgaaccactt gtttgcaaac gggcatgact cctgacttat atttctgcct
     1081 tttattcctt ttacacttgt ttttatgaag cccttcacag aattgtcctt tcacgatgcc
     1141 gtctctctga tgattgatgt taattaacaa tgcatttacc aaaaacaaac atataaatca
     1201 caggagtcgc ccatgtcagt acccgttcaa catcctatgt atattgatgg acagtttgtt
     1261 acctggcgtg gagacgcatg gattgatgtg gtaaaccctg ctacagaggc tgtcatttcc
     1321 cgcattcccg atggtcaggc cgaggatgcc cgtaaggcaa tcgatgcagc agaacgtgca
     1381 caaccagaat gggaagcgtt gcctgcaatt gaacgcgcca gttggttgcg caaaatctcc
     1441 gccgggatcc gcgaacgcgc cagtgaaatc agtgcgctga ttgttgaaga agggggcaag
     1501 atccagcagc tggctgaagt cgaagtggct tttactgccg actatatcga ttacatggcg
     1561 gagtgggcac ggcgttacga gggcgagatt attcaaagcg atcgtccagg agaaaatatt
     1621 cttctgttta aacgtgcgct tggtgtgact accggcattc tgccgtggaa cttcccgttc
     1681 ttcctcattg cccgcaaaat ggctcccgct cttttgaccg gtaataccat cgtcattaaa
     1741 cccagtgaat ttacgccaaa caatgcgatt gcattcgcca aaatcgtcga tgaaataggc
     1801 cttccgcgcg gcgtgtttaa ccttgttctg gggcgtggtg aaaccgttgg gcaagaactg
     1861 gcgggtaacc caaaggtcgc aatggtcagt atgacaggca gcgtctctgc aggtgagaag
     1921 atcatggcga ctgcggcgaa aaacatcacc aaagtgtgtc tggaattggg tggtaaagca
     1981 ccagctatcg taatggacga tgccgatctc gaactggcag tcaaagccat cgttgattca
     2041 cgcgtcatta atagtgggca agtgtgtaac tgtgcagaac gtgtttatgt acagaaaggc
     2101 atttatgatc agttcgtcaa tcggctgggt gaagcgatgc aggcggttca atttggtaac
     2161 cccgctgaac gcaacgacat tgcgatgggg ccgttgatta acgccgcggc gctggaaagg
     2221 gtcgagcaaa aagtggcgcg cgcagtagaa gaaggggcga gagtggcgtt gggtggcaaa
     2281 gcggtagagg ggaaaggata ttattatccg ccgacattgc tgctggatgt tcgccaggaa
     2341 atgtcgatta tgcatgagga aacctttggc ccggttctgc cggtagtcgc atttgacacg
     2401 ctggaagatg ctatctcaat ggctaatgac agtgattacg gcctgacctc atcaatctat
     2461 acccaaaatc tgaacgtcgc gatgaaagcc attaaagggc tgaagtttgg tgaaacttac
     2521 atcaaccgtg aaaacttcga agctatgcaa ggcttccacg ccggatggcg taaatccggt
     2581 attggcggcg cagatggtaa acatggcctg catgaatatc tgcagaccca ggtggtttat
     2641 ttacagtctt aatgagtgaa agaggcggag gttttttcct ccgcctgtgc gcgtcagagt
     2701 ttagcgaatt tttcgagggt gcgaataagc tgcgtgacga agccatattc gttatcgtac
     2761 caggcgaccg ttttcaccag ttgtaaatcg cccacggcgg taatttccgt ttgcgtggca
     2821 tcaaacaccg aaccgaaatg gctgccaatg atatcggaag agactatttc ttcatcggta
     2881 taaccaaatg actcgttatt ggtggttgct tgtttaagtg cgttattcac ctcttcggca
     2941 gtcacttttt ttccgagaat cgataccagt tcagtgaccg aacctgtttt caccggcacg
     3001 cgttgcgcat gacctttcag tttgccgctc agttccggga tcaccagacc aatggctttg
     3061 gccgcgcccg tagtgtgggg aatgatattt tctgccgctg cgcgtgaagc acgtaaatct
     3121 ttaccacgcg ggccatccac cagtgactgg gtgccagtat aggcatgaat ggtcgtcatc
     3181 gtgccgactt ctatcccgaa actgtcatgc aaggctttgg ccatcggcgc aaggcagtta
     3241 gtggtgcatg acgccacgga aacaatggta tcgttgccat ccagagtgtc gtcattgacg
     3301 ttataaacga tagttttcat ttcaccggca ggggcggaaa tcaacacctt cttcgcgcca
     3361 gcatcgagat gcgcctgcga tttctcggcg gaggtataaa agccagtaca ttcgacaatg
     3421 atttctgcac ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg
     3481 atacttttcc cattaacgat aagtgaatct tccgtaaaat caacgctcca ggggaacggt
     3541 ccgtagtttg aatcatgttt cagcaggtag gcgagaattt ttggagaagt gagatcatta
     3601 atagcgacaa cgtctatgtt gcttttgact tcaagtaatc gacgcaacac cagtcgaccg
     3661 atacgaccaa aaccgttaat accaacttta ctcatggttt tctcctgtca ggaacgttcg
     3721 gatgaaaatt gatcctttcc aggcttagac caggatggcg ggatgggcaa tctccattct
     3781 tacagtgaaa cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat tttgagaaaa
     3841 gcccgttatg ttaacggaaa attatgttaa agcaggaaat gttatggaaa ataaatactc
     3901 aaggttacaa atcagcattc actggctggt ctttttgctg gttatcgcag cgtattgcgc
     3961 aatggagttt cggggtttct taccacgtag cgatcggcca ctcatcaaca tgattcatgt
     4021 ttcctgcggc atatcaattc tcgtgctgat ggtcgttcgt cttttgttaa ggctgaaata
     4081 cccaaccccg ccgattatac ctaagccaaa accgatgatg acgggactgg cgcatttggg
     4141 acatttggtg atttatctcc tgtttattgc gctgccagtg attggtttgg tgatgatgta
     4201 taaccgaggc aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt cagaggccaa
     4261 tttcgaacgg gttgatagct taaagtcgtg gcatgaaacg ctggcgaatc tgggatattt
     4321 tgtcatcggt ttgcacgctg cggctgcact ggcacaccac tatttctgga aggacaacac
     4381 acttctacgc atgatgccgc gtaaacgttc ctgaaggata tttaaagaaa acgcctgtac
     4441 taaaaccgac ccgtggtaca ggcgaagaat acgggtctac atcggaagcg cctattatat
     4501 ttatttgtat gacaaataaa accataatcc ttgcccatac gtccatctgg cttattttta
     4561 atcaattcac ccgatctttg atctcatcaa cggtatcaaa ataaaccagc gtataacgtt
     4621 caaatcgaac ataagattcg ataaaccatg gaggttatat gaaaaaactg gcacttattt
     4681 tgtttatggg aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc tgttctggtt
     4741 cgaaaggggg gatctcacac tgtacggcag gcggcaaatt tgtctgtaat gatggttcta
     4801 ttagtgcatc gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca tgagaaaatg
     4861 aatctgctag tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg gacgtgcaag
     4921 ccatgagcgc agcaacctcc ttatcaccgt cccggaaccg cagttcgtag agcgtttgtc
     4981 gggtcaggag tgtgaatgtc agaatcgtaa tgcagataat gagcagacac accaccagag
     5041 ggttgcgctt catagccttt ctccttgccg gatggcgggt aagaggctaa catctgaatt
     5101 gcttggttca ttcgttggcc tcggttgata gaaatatcgg tcggggcctt cgtctttctg
     5161 attcccggtt agcctgaaaa cagaaagtct caggcacccg tgagcatcct atgtggtttc
     5221 cttagggacg aaaataatca cctgacgaaa ttgcgtgctg ttttccagaa tttttcgtca
     5281 ttcggtttag ccattcgtta ctctcttcat tccaatagca ttaatcttct ctgcaataat
     5341 tgttgtaaaa atgtgacgca aagaggcttt tagtcgtaag taattaccgt caaatgccga
     5401 tgactttcta tcaggagtaa acctggacga gagacaacgg taatgaatac aactccctca
     5461 cagcgattag gttttttgca tcacatcagg ttggttccgt tatttgcctg cattctaggc
     5521 ggtatcttag ttctattcgc attaagttca gccctggctg gctatttcct ctggcaggcc
     5581 gatcgcgatc agcgtgatgt tactgcggag attgagattc ggaccgggtt agcgaacagt
     5641 tcagattttt tgcgttcagc ccggatcaat atgattcagg ccggggctgc gagtcgtatt
     5701 gcggaaatgg aagcaatgaa gcgaaatatt gcgcaagccg aatcggagat taaacagtcg
     5761 cagcaaggtt atcgtgctta tcagaatcgg ccggtgaaaa cacctgctga tgaagccctc
     5821 gacactgaat taaatcaacg ctttcaggct tatatcacgg gtatgcaacc tatgttgaaa
     5881 tatgccaaaa atggtatgtt tgaagcaatt atcaatcatg aaagtgagca gatccgaacg
     5941 ctggataatg cttataccga tattttgaac aaagccgtta agatacgtag caccagagcc
     6001 aaccaactgg cggaactggc ccatcagcgc acccgcgtgg gtgggatgtt catgattggc
     6061 gcgtttgtgc ttgccctggt catgacgctg ataacattta tggtgctacg tcagatcgtc
     6121 attcgtccac tgcaacatgc cgcacaacgg attgaaaaaa tcgccagtgg cgatctgacg
     6181 atgaatgatg aaccggcggg tcgtaatgaa atcggtcgct taagtcgtca tttacagcaa
     6241 atgcagcatt cactggggat gacagtaggg actgttcgac agggcgcgga agagatttat
     6301 cgtggcacca gcgaaatttc cgctggcaat gcggaccttt catctcgcac cgaagaacaa
     6361 gcggcggcta tcgaacaaac tgccgccagc atggagcaac tcactgcgac ggtgaaacag
     6421 aatgcggata acgcgcatca tgccagcaaa ctggcgcaag aggcttctat taaagccagc
     6481 gatggcgggc agacggtttc cggggtagta aaaacgatgg gcgctatctc cacgagttcg
     6541 aagaaaattt ctgagatcac cgccgtcatc aacagtattg ctttccagac gaatattctg
     6601 gcactgaatg ctgccgttga agccgctcga gcgggtgagc aaggacgtgg atttgccgtt
     6661 gtcgccagcg aagtacggac actcgcaagc cgcagcgctc aggcggcgaa agagattgaa
     6721 ggcttgatca gtgaatcagt caggttaatt gacctggggt cgaatgaggt ggcaacggca
     6781 gggaaaacca tgagcactat tgttgatgcc gtcgcgagtg tcacacatat catgcaggaa
     6841 atcgccgccg cctcggatga acaaagtaga ggcataacgc aggttagcca ggcgatttct
     6901 gaaatggata aggtgacgca acagaatgct tctctggtag aagaggcctc agcagcggcg
     6961 gtgtcccttg aagaacaggc tgcacgatta actgaggcgg tggacgtatt ccgtctgaac
     7021 aaacattctg tgtcggcaga acctcgcgga gcgggtgaac aagttagttt cgctacggtg
     7081 tgaaaatgtt caaggaggga tcgacagatc ccttcacctt tcagaacggc attgattttc
     7141 gaatagcgtt aatcatcaac tggcaaccag aagagaacgt cgcatctacg cgggtcagta
     7201 ttccaatcgg ttcgcctgca ccatgtcccg gaacaggcag ggccaccagc gtggcatgac
     7261 gcaggtcgtc ttttaccgcg ccagaaggga caaaccacac gtagtcgtat tcaaccgtaa
     7321 gttgacgaga tagcgaagca gacagcgttt cgatacaacc cgaaggaatt ttacatccct
     7381 ggctctgtac taatgcatct gaatgctggc gtggtgcagt gccttctggt gatacaacga
     7441 ccggccattc cagcacccgg cttagcgtta cgttctcctg aagtagcggg tgattagggc
     7501 ggacaaccag cttcagcgat tcaagaaaca gcagttcgta attaagcccg gtcatcagtt
     7561 caggatctga catccgacca atgccgatat cgatttcccc ggtttttaaa cccgccagaa
     7621 tcatagggtt actcattgtc gcaacttgca aggtcgtctc tttttgttgc tgatgaaact
     7681 gacctataac cgaaggtaat atccccagtg ccgcagtagg tagtgcacca accctgacga
     7741 catcattatt aagaccttct ttacgatgaa gcgactgtcc agcagtgttg atggcgtcaa
     7801 gaactctgac tgcatgcgtt aaaaattgtt cgccgggtaa ggtaagttgc gccccctgac
     7861 gaccacgctc aaacaagcga gcgcccgtca gttgctccag ttcattcaat gtcttagaga
     7921 gcgcaggttg actcaaatta agggtttcag ccgcgcgccc caaagttcct tgttgtgcga
     7981 cagctacgaa tgtatgaagg tggcgcaaac ggatgcgctg actaaacaga ctattttttt
     8041 ccataagcga tgttaaaaac gaagcggtgt cgctgacaag tgaagttgtt tgattatgat
     8101 aacttgattg c
//
LOCUS       DAZUCE010000222         8105 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZUCE010000222 DAZUCE010000000
VERSION     DAZUCE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.222"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..477)
                     /locus_tag="ACHY03_004203"
     CDS             complement(<1..477)
                     /locus_tag="ACHY03_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFS3907375.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINE"
     gene            complement(477..884)
                     /locus_tag="ACHY03_004204"
     CDS             complement(477..884)
                     /locus_tag="ACHY03_004204"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFS3907376.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNTNVEAVEFVPGENAGDIV
                     AKLKKLVSIHSQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            complement(894..1700)
                     /locus_tag="ACHY03_004205"
     CDS             complement(894..1700)
                     /locus_tag="ACHY03_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3907377.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(1770..2717)
                     /locus_tag="ACHY03_004206"
     CDS             complement(1770..2717)
                     /locus_tag="ACHY03_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFS3907378.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            3065..3937
                     /gene="rluF"
                     /locus_tag="ACHY03_004207"
     CDS             3065..3937
                     /gene="rluF"
                     /locus_tag="ACHY03_004207"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFS3907379.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(3938..4210)
                     /locus_tag="ACHY03_004208"
     CDS             complement(3938..4210)
                     /locus_tag="ACHY03_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFS3907380.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4463..5812)
                     /gene="lysC"
                     /locus_tag="ACHY03_004209"
     CDS             complement(4463..5812)
                     /gene="lysC"
                     /locus_tag="ACHY03_004209"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFS3907381.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     regulatory      complement(5904..6100)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            6337..7986
                     /gene="pgi"
                     /locus_tag="ACHY03_004210"
     CDS             6337..7986
                     /gene="pgi"
                     /locus_tag="ACHY03_004210"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFS3907382.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1992 a         2110 c         1986 g         2017 t
ORIGIN      
        1 ttcgtttatt ttgtcgataa tattgattga gggatctgac gctacgacgc gtaaatccag
       61 tatgacgcca agattattta actcatgaaa tgcgttaata tcatcatcat ctaatgaaac
      121 cgctttggtt aactgttttt taccgggccg ccaggccatg ccgccaatat ttagggtgcc
      181 tatttttacc ccctggcgaa ccatcgctaa ggcatcctgt gggcgagtga aaagataaaa
      241 aacggtttcg tcctgatatt gcgggttgtg ataaaccgcg acggcttttt caatattgac
      301 cacgttaacc ttcattcccg gtggcgcagc ctgacgcaat aatgtccggc gaacttcatc
      361 gttataaact tcgtcattgc agataataat gcgctgggcg ttagccactt tcgaccagac
      421 ggtagtgacc tgaccgtgga tcaggcggtc atcaatgcgg gcgagcgtaa tgttcatcag
      481 aaatcctcct ctgtcgttgc cagttgtttc cagacaacgc aggtttgctt tgcgatttgc
      541 gtcaggtgtt cacacaattc atcaacattc atgctgtcct ggttatcaac cagttcgagc
      601 gccagcggca gagaaagtcc gctgattacc cgcagacgag gattgcgcat cgcaagcatc
      661 gctgccgcat tccacgggct gccgcactgt aaatccacgg caatcagcca ctcatcttga
      721 ctgtgaatgc ttactaactt tttcagcttt gctacgatgt ctccggcgtt ttcaccgggc
      781 acaaattcaa cggcttcaac attggtgttg ccgtaaacca tctgaaccga ttccagcatg
      841 gcgcaagcta atttgccgtg ggcacaaaaa atggcgttga ccataaagcc tccttatccg
      901 cgcgttttgc cgcccgcaat gttagtggtt actccggtga tgtagctggc gcgttcagac
      961 aacagataac aaacaaaatc agcgacttct gctaatcttc cggcgcgccc aataggaatg
     1021 gcgtttttgg tatagccttc acgcagctgc tcgacggtga tattgcgcgt ccacgccagc
     1081 gcctcttcat attccggcgt gcgtagcccg gttttttcca gaatcccggg cgcgataccg
     1141 accacacgga taccgtgttt acccagctct ttcgaccagg agcgcgtgaa gctattgagc
     1201 gcggctttgg tagcggcgta acagctttgg ccttctgagc cttccagtcc actttctgag
     1261 gaaacattca caatcacgcc atcatgttgt ttgaccatct gtcgcgccac cgcctgcgac
     1321 atcagaaaaa cgcctttctg gttgatattg accatttttt cgaatgcagc ttcgttgagt
     1381 tcatactgcc cggcaggcgc tttctcatcg accagcagac gcgggaaatt gaccccggcg
     1441 ttattgacca gaccgtcgat gcgaccaaaa cgctggataa tttccgctac cgtatgattt
     1501 acctctttgg cgctggaaat atcggtcggc caaaactgat agcctttatg aacttcatat
     1561 tgaccatcgc caccgtgaat atcgaccatc tgtacgtttg cgccttgtgc taataattcc
     1621 tctacaatcg ccagaccaat accggatgcg ccgccggtga cgataatgat tttatcctgc
     1681 aaatttaacc acgtttgcat aacagactcc tgattttaga taatagggac ccgccgtaat
     1741 ttttattacg ataatcatcg ggaagatttt tatttcagta atagttcagc tgtgctgcta
     1801 ttcgttacca gacaattaat atattttcca cgcagtgcac caataattcc gctgtatttt
     1861 tcttcactca tggcaatgcc aatggaatat cgtgcctgct ttaatttatt catttcgata
     1921 gagagtgttt tttcgctcat attcgtttca accattgcgc cgtgaatatc aaaaaagcgc
     1981 gagcaaatat cgccagcaac ctggcgggca ttcaggtcgt cactctcttc accaccataa
     2041 aacgcatgcc agttagcgcc gtcgcgaatg gccggtgagc caattcccac cagggcgaca
     2101 tccagattat cccagtaggc agagatggtt ttaaagtgct gagactgcat gatcccatta
     2161 cgaattaagg ggttatccag cagagccgga aaatccgcga gatgcgattc ccctttcagc
     2221 ttcgcagccg cgctgtaggt taatgtgttt acgtgatagc ggctttcgag tttaccggac
     2281 ggtccgccaa taattggtac gcagattaac tgccgcgatt gccccgcctg cggcaagttt
     2341 tcaaccagtg cgctcaccgc gcgaccccag gaaaaaccga caatatcgcc aggctccagc
     2401 aagcgatcca gcagttgcgc gccgtgtaac cccatcatcg ccagttgagt ctcttcatcc
     2461 tcatcatttc ccgacaccac cacaacgtct ttcaggccaa acttttgctt cagttgctgc
     2521 tccagccaga gattttcgtt gtagtcatag ttgatggcga tggtgacaat tccctgatcg
     2581 cggcctcgtt taagcaagcg gctgatggtg gtgcggtaaa tacccagttc gcgcgcgatt
     2641 tgcgcctgcg tcatatcctg ttcgtaatag agttgggcaa tcttcacaat caaacggata
     2701 tcgtcactgt tttccattgc cgcctcctgg ttctgcacat ttgtgcaaat acgctttctc
     2761 accgcttgat ctgatataac cccatcatga tggcctttca aagcgatttt tcactctttg
     2821 catggggaga aatagcgctt tctcttgcac atttgtttct ctgcgtagta gttatcagca
     2881 caggtgtgca gcgattcgta atatgttgat tatccttatt ttgacaggca aatttgcatt
     2941 gtacttccgg agacactttt gtgataaaag gcgtggcaga tcacatttaa tttcgacgtt
     3001 gccgcataac cctgtttttc cccgctaaga ggtacggtat attccgcgtt ttacaggaga
     3061 atttatgctg cccgactcat cagtccgttt aaataaatac atcagcgaaa gcggaatttg
     3121 ctcacgccgc gaagcggatc gctatatcga gcaaggcaat gtgttcctta atggcaagcg
     3181 agccaccatt ggcgatcagg tgaaacccgg cgacgttgtg aaagtaaacg gtcagttgat
     3241 tgagcctcgg gaagccgaag atttggtact tatcgccctg aacaagcccg tcggtattgt
     3301 cagcaccacc gaagatggcg agcgcgataa cattgtcgat ttcgttaacc acagcaaacg
     3361 cgtgttccca attggccgcc tggataaaga ctcccagggg ctgattttcc tcaccaatca
     3421 cggcgatctg gtgaataaga tcctccgtgc gggtaacgat catgagaaag agtatctggt
     3481 gacggtcgat aaaccgatta ccgatgaatt tattcgtggt atgggggcgg gggtgccgat
     3541 cctcgggacg gtgactaaaa agtgcaaagt caaaaaagaa gcgccgtttg tcttccgcat
     3601 taccctggtg caggggctga accgtcagat ccgtcgcatg tgcgagcatt tcggctatga
     3661 agtgaaaaag ctggaacgta cgcgcattat gaacgtcagc ttaagcggca ttccactggg
     3721 ggagtggcgc gatttaaccg acgatgagtt aatcgacctc tttaagctca ttgaaaattc
     3781 ctcttccgag gcaaaaccta aagcgaaggc taaaccgaaa acggcgggca tcaaacgtcc
     3841 agtcgttaag atggaaaaaa cggcagaaaa aggcggtcgc ccggcgtcca acggtaagcg
     3901 ttttacctcg ccggggcgta aaaagaaggg gcgctaatca gcgcctgccg cgcgtcgacg
     3961 tatttgctga ccagctaaat gacgcttcag gatccggttt ataagcctgc tttttcttta
     4021 actggcgggc ttttttcgct tcagcctcac gctcgaccat taatttatcg atgtactctt
     4081 tcttaatgct gttggtttcg gagttggtca gcacgcgacc atgtgcaatc cgcgcccgat
     4141 ccagcagtgt tttaagttca cgctgctcgc gttcagtcat ctctttttgg gtaatacggg
     4201 ggagtgccat gatggtgccc tcagtgagcc agagagttca gtgtacggga attcgtttgc
     4261 gagcagaact gataatattc attttgcctt ctcttaacta tttatctctt tcgataatta
     4321 attagagatg cagataaaaa aattaaaggc aattattctc cgataaagac gctggttaac
     4381 agagtacagg ctcgtttatt gatgagcata gtgacaagaa aatcaatacg gcccgaaata
     4441 tagcttccag gccatacagt atttactcaa acaaattaaa atgcagcttc tgcaccacct
     4501 gctcggcatc ttcgccgggc accaggaagc acaggttatg gctggatgcg ccataacaaa
     4561 tcatgcgaat gttgaacggt tccagtacgc cgaatacctc tttgccaacg ccgcaggctt
     4621 ttgacaggtc attgccaatc aacgcgacca gcgccagacc ttcttccacc tccacccggc
     4681 acagtgcgga aagctccatc agcagagatt gcgtcagcaa cgtatcgcca gtggaggttg
     4741 aaccggtggt atcaagggtt aatgccacgc tcacttctga cgtggtgatt aagtctaccg
     4801 aaatattatg ccgcgcgagg atgccgaaaa cttccgcgag gaagccgcga gaatgcagca
     4861 tattcaggct gtgcaaagtg agcagagtct gattgcgacg aagcgccagc gcgcggaaca
     4921 gcggcggatt ttcagtttta ttgcacacca gcgtaccacc tgcgcgtggg tctttgctgg
     4981 agccgacaaa gaccgggata tcgctgcgta ctgcgggtag caacgttgcc ggatgcagta
     5041 cttttgcacc aaaagttgcc atctctgccg cttcggcaaa cgcgatttca tcaatgcgtt
     5101 ttgctgcgga aactacgcgt ggatcggtgg tgtagatgcc cgggacgtcg gtccagatat
     5161 caacacgaga tgcgtgtaaa gcctccgcca gcaaggctgc cgtataatcg ctgcctccac
     5221 ggccaagcgt cgttgtacgg cctttatttt cgctaccgat aaatccctgg gtgatcacta
     5281 agccttcatt gagacgtggg agcagctgca gcgcggccag ttccgccagc gcggctatat
     5341 ctggctctgc acgaccaaat cggtcgttgg tacgcatcac tttacgtaca tcaaaccact
     5401 gtgcctgaac atcgcgttcg cgcaggatct caacaaacag cagggtcgac atcagctcgc
     5461 cgtggctgac cagctcatct gtcagcgccg gagacgttgc cagcgccgcc gcttctgcca
     5521 gaacagtaat gttctccagc agacgttcaa tctcttcacg gataacgttc gggtaacgca
     5581 gacgttccag aatggcaaac tggatgttgc ggatagcgtc gagtttttcg aatcgctcgc
     5641 caggttccag tccttcagct aaagcgacca gcagattagt gataccagca gaagccgaga
     5701 ggacaactaa acgcacgttg gcatcagaaa gcacaatatc agcgctgcgg ttcatggcgt
     5761 caaaatcagc tacgctggta ccgccaaatt tggagacaac aatttcagac ataactacct
     5821 cgtgtcaggg gatccatttt cagccttggc acaagggaag agcggaagac gggtgggcgc
     5881 agagcgatac ttcgctacta ttttcaccca gaagtgctcc accacttgcg aaacgcccga
     5941 ctgcgaacgc ttctggtgac aacccagggg attcagcccc tgtagccgat gatgaacgtg
     6001 gccagccgtt caatcacctc ggcgatgcac cccctcaggt gttatcacag gactggctcc
     6061 tccaacaccg ttacttgggc aacgcgcctc ttctggcctg cgctagcgca ggtagtacat
     6121 ttataaataa agggtgagcg gggcggttgt caacgacggg gtcatgcgga tttttcatcc
     6181 actcctggcg gtcagtagtt cagctaataa atgcttcact gcgctaaggg tttacactca
     6241 acattacgct aacggcacta aaaccatcac atttttctgt gactggcgct acaatcttcc
     6301 aaagtcacaa ttctcaaaat cagaagagta ttgctaatga aaaacatcaa tccaacgcag
     6361 accgctgcct ggcaggcact acagaaacac ttcgatgaaa tgaaagacgt tacgatcgcc
     6421 gatctttttg ctaaagacgg cgatcgtttt tctaagttct ccgcaacctt cgacgatcag
     6481 atgctggtgg attactccaa aaaccgcatc actgaagaga cgctggcgaa attacaagat
     6541 ctggcgaaag agtgcgatct ggcgggcgcg attaagtcga tgttctctgg cgagaagatc
     6601 aaccgcactg aaaaccgcgc cgtgctgcac gtagcgctgc gtaatcgtag caataccccg
     6661 attctggttg atggcaaaga tgttatgcca gaagtcaacg cggtgctgga gaagatgaaa
     6721 accttctcgg aagcgattat ctccggtgag tggaagggtt ataccggcaa agcaatcact
     6781 gacgtagtga acatcgggat cggcggttct gacctcggcc cgtacatggt gaccgaagca
     6841 ctgcgtccgt acaaaaatca cctgaacatg cactttgttt ctaacgtcga tgggactcac
     6901 atcgctgaag tgctgaaaaa agtaaacccg gaaaccacgc tgttcttggt agcatctaaa
     6961 accttcacca ctcaggaaac catgaccaac gcccatagcg cgcgtgactg gttcctgaaa
     7021 gcggcaggtg atgaaaaaca cgttgcgaaa cactttgcgg cgctctcaac caatgccaaa
     7081 gccgttggcg agtttggtat tgatactgcc aatatgttcg agttctggga ctgggtcggc
     7141 ggccgttact ctttgtggtc agcgattggc ctgtcgattg ttctctccat cggctttgat
     7201 aacttcgttg aactgctttc tggcgcacac gcgatggaca agcatttctc caccacgcct
     7261 gccgagaaaa acctgcctgt cctgctggcg ctgattggca tctggtacaa caatttcttt
     7321 ggtgcggaaa ctgaagcgat tctgccgtat gaccagtata tgcaccgttt cgcggcgtac
     7381 ttccagcagg gcaatatgga gtccaacggt aagtatgttg accgtaacgg taacgttgtg
     7441 gattaccaga ctggcccgat tatctggggt gaaccaggca ctaacggtca gcacgcgttc
     7501 taccagttga tccaccaggg aaccaaaatg gtaccgtgcg atttcatcgc cccagctatc
     7561 acccataacc cgctctctga tcatcaccag aaactgctgt ctaacttctt cgcccagacc
     7621 gaagcgctgg cgtttggtaa atcccgcgaa gtggttgagc aggaatatcg cgatcagggt
     7681 aaagatccgg caacgcttga ctacgtggtg ccgttcaaag tgttcgaagg taaccgcccg
     7741 accaactcta tcctgttgcg cgaaatcact ccgttcagcc tgggtgcgtt gattgcgctg
     7801 tatgagcaca aaatctttac tcagggcgtg atcctgaaca ttttcacctt cgaccagtgg
     7861 ggcgtggaac tgggtaaaca gctggcgaac cgtattctgc cagagctgaa agatgataaa
     7921 gaaatcagca gccacgatag ctcgaccaat ggtctgatta accgctataa agcgtggcgc
     7981 ggttaatcat cgttcatgcc tgataccgct atgtaggccg gataaggcgt ttacgccgca
     8041 tccggcaact ggtgcctgat gcgacgcttt acgcgtctta tcaggcctac aggtcgatgc
     8101 cgata
//
LOCUS       DAZUCE010000223         8078 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZUCE010000223 DAZUCE010000000
VERSION     DAZUCE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.223"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(22..711)
                     /gene="yjcO"
                     /locus_tag="ACHY03_004211"
     CDS             complement(22..711)
                     /gene="yjcO"
                     /locus_tag="ACHY03_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFS3907383.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(805..2952)
                     /gene="fdhF"
                     /locus_tag="ACHY03_004212"
     CDS             complement(805..2952)
                     /gene="fdhF"
                     /locus_tag="ACHY03_004212"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2533..2535),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFS3907384.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(3150..4616)
                     /gene="mdtP"
                     /locus_tag="ACHY03_004213"
     CDS             complement(3150..4616)
                     /gene="mdtP"
                     /locus_tag="ACHY03_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFS3907385.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAQGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRATSNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(4613..6664)
                     /gene="mdtO"
                     /locus_tag="ACHY03_004214"
     CDS             complement(4613..6664)
                     /gene="mdtO"
                     /locus_tag="ACHY03_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFS3907386.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQVLNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQYAITEGQCWQSDWQISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(6664..7710)
                     /gene="mdtN"
                     /locus_tag="ACHY03_004215"
     CDS             complement(6664..7710)
                     /gene="mdtN"
                     /locus_tag="ACHY03_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFS3907387.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEM
                     FRIGASAVANLEPQ"
     gene            complement(7729..8004)
                     /locus_tag="ACHY03_004216"
     CDS             complement(7729..8004)
                     /locus_tag="ACHY03_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFS3907388.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         1851 a         2208 c         2132 g         1887 t
ORIGIN      
        1 acgcgtctta tcaggcctac cttaaccgtt agttaatttt tcaaactctt cacacccggt
       61 atcaaaccct tccatacagc tcaggttcaa ccagtgcagc gccttttgtt tattcttctc
      121 gataaaacct tcttcaccgt ttaagaacat cattcccgcc cagtactcgg aataaccggt
      181 tcgggaaatt gcagagctgc gtttgaaata ccaggttgcc ttgtcatcgt cggccttaat
      241 gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg gcatcgacgg cggagtcatt
      301 ctccagatct tccgaggcgt tttctaacag cgaaatcgcc tttggataat ccggtttgcc
      361 cgcttgagta tttaccagaa tatgcgccag cgttacttca ccttctttac tgcccgcttg
      421 cgccgctttc tctgcaagaa cttttgcttg tggatagtcc agactgaccg gattggtgat
      481 tttgatctgc gccagcagcg cgcaggcatc agcatcaccg ctgtcggcgg ctttctgtgc
      541 ccaatattcg gctttgctca aatcgccgga gctaaaccag ctgtcggcaa gaaaatattg
      601 tgcgcgtcgg tccccggcct ctgctgcctt taaatactgg ctgccaggct cggagtcgtt
      661 ggcgtgggca aagaatgtca aaaacaacat caatgcgata atttttttca ttttgataat
      721 acgattgggt agggagtaac cagtataaag agttcgaatg gataaaaaaa cagcctccga
      781 aaggaggctg tagaaaggac tgtattacgc cagtgccgct tcgcgcaggc gagttttcaa
      841 cttgttgtac tcgtcaatca cgtactgctc ggcggcacgc tgatcggcga tcggctcgac
      901 gcgcacggcg cagtatttgt attccggcgt tttcgtaatc gggcttaagt tttcggtaac
      961 cagctcgtta caggcaccaa tccaccactg gtaggtcatg taaatcgccc ctttgttcgg
     1021 acgatcgctg acctgcgcac gggtgataat tttgccttta cgcgagtgca cccaaaccaa
     1081 tgcctcatct tcaataccca gacgtttggc gtcttcggta ttgatttgtg cgtagccagg
     1141 ttcatccgcc agcgccgcca gtgccgcaca gttaccggtc atcgaacggc aagagtagtg
     1201 accaacttca cgcaccgttg acagtaccat cgggtactcg tcggtgagtt tgtcgattgg
     1261 cgctacccag tcgcaggtga agaactgcgc cagaccgttc ggggtatcaa acttctcttt
     1321 aaacagataa gaagtcccct gatcggcatc tgaagtatcg cggcaaggcc actgaatgaa
     1381 gcccagttcg cccattttct cgtaagtcgc accgtagaaa tccgggcaca gatgacgcaa
     1441 ctcatcccag atctcctggg tgttgttgta gtgcatcgga taacccatac gggtggcgat
     1501 ttcactgatg atttgccagt ccgttttcag atcccatttc ggttcaaccg ccttgaagaa
     1561 acgctggaag ccacggtcag ccgcagtaaa cacgccttca tgctcgcccc acgacgttga
     1621 cggtaaaata acatccgccg ccgacgcggt tttggtcata aagatgtcct gaacgatgac
     1681 cagttccaga tcttcaaagg ctttacgtac tgccgacagc tccgcgtcag tttgtagcgg
     1741 atcttcgccc ataatgtacg cggcacgcac ttcgccatgc gctgcgcggt gcggcagctc
     1801 gctgatgcga taaccggtat gagcaggcag gctttccacg ccccaggctt tggcgaattt
     1861 ctcgcggtta gccggatctt tcacgtactg gtatcccgga tacgtatccg gcagcgcgcc
     1921 catatcgcag gcaccctgca cgttgttctg accacgaacc gggtttacac ccgcatgcgg
     1981 cttaccgagg ttaccggtca gcatcgcgag gctggtcaga gaacgcacgg tttccacgcc
     2041 ctggtagaac tgggttacac ccatgcccca caggatggcg gcgcttttcg cctgggcata
     2101 catccgtgcc gcctgacgaa tctcactggc gctgacgccg gtgatatctt caaccgactc
     2161 cggcgtgtag ccttcaacga ttttacgata ctcttcaaag ccttctgtac gggaagcgac
     2221 gaacgctttg tcgtacagat tttcttcaat aatgacatgg cccatcgcat tcaacagcgc
     2281 gatgttcgag ccgtttttca gtgcaatgtg catgtcagca atgcgcgcgg tttcaatttt
     2341 gcgcggatcg cagacgataa ttttcgcccc gttacgttta gcgttaatta cgtgattcgc
     2401 cacgattggg tgggaatccg ccgggttgta cccgaaaacg aacactaaat cggtattatc
     2461 aatttcgtta atagcattgc tcattgcgcc attaccgacc gattggtgca gacctgcaac
     2521 cgatgggccg tgtcagacac gagcgcagca gtcaacgtta ttggtaccaa taacggcgcg
     2581 cgcaaatttt tgcattacat agttggtttc gttacccgta ccacgcgagg agccggtcgt
     2641 ctggatggca tccggaccgt acttctcttt gatggcgctc aggcgctcgg caacgtaatt
     2701 cagtgcctca tcccaggaaa caggttcgag tttgccgcca cgctggcgac ggatcatggg
     2761 ggttttcagg cgcggggtca ggatctgggt atcgttaatg aaatcccagc cataataacc
     2821 cttcagacac agggtaccct ggttggtttt cccctgcgct gcctccgccc ggacgatttt
     2881 gccgttatcg acgaccaggt tgattttgca acctgatgcg caataggggc aaaccgtgac
     2941 gacttttttc atcggtctcg ctccagttaa tcaaatcacg catacgcgct ctcgactaca
     3001 gtatgcatct tttatgccac attttatttg gggtcattcc ctgatattac gggcactatt
     3061 tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt gacgtgacga aacgaaatac
     3121 cgcgtgacag ccatcacgcg gcagacattt tattttttct cgacgacggg agccgcctga
     3181 tacccgccgc ccagcgattt catcaactga atgctttgga tcacccggcg gctgtccagc
     3241 atcagtaatg acatctcttc ggcaagtact ggcaaccggg cttcagtggc ctgtaagcgg
     3301 ctggttaagc cgcgctgata ggcggcctcg gcagcgcgct gggtgaagcg cgttgcttcc
     3361 acgcgttcag cctgcatttc tcgctcgtcg ttgagcgttt gcagacgcgt gccgttgacg
     3421 gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac gttcaatcat catgttgctg
     3481 gtggcgcgcg tgccttcgag attggcattc aaccgtccac cgtcaaacag cggcaatttc
     3541 agacccggga taaagttgaa ctggcgactg gtttttttga ataaggtatc caggtggatg
     3601 gcatcaaggc cgaaaaacgc tttgatatca aagctcgggt agaacaacgc ccgcgcggaa
     3661 tccacctgat ctaatgacgc ctgaacatac cagcgcatgg cttgcagatc cgggcgtctg
     3721 gcgagcaact cataagagag tgtcgccgga atgccggtct gaactcgtgg taatgctaca
     3781 ggtttgatct ccggcatatc gctggctccc gcgccaatca atgcacgcag agattctcgc
     3841 gtttcggtga tttgcccttt gacggcagca atttgtttat cgaccgccag aatctgtgcc
     3901 cgcgcgccgt ggaaaggcac ttgcgcttcc agaccttgcg ccactttact ctggtgcgct
     3961 ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca gatcgagcat ctgatagctg
     4021 gcctgcatac tgtaataaag ctgcgctacg cccgtggtca gcgatagctc tactgctgcg
     4081 gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg caaccgctga gcgatgcaca
     4141 ccccacaaat caagatccag tccggcaaac aaacctactg tggcttccgt atagtacggc
     4201 ccgtccatac ccagtgctgg cgcatccatc gcataagggc ttaaaaagcc gttcgccgag
     4261 acgcgttggc ggttgagcat ccctaacgct gcgacctgta attgtgaacc ggcatctaac
     4321 aaatcggcct gggactgcgc tttttcttcc cgcagtttcg cttctgcgag ggtgtgtgaa
     4381 ccacttagcg tccgctggat cagggcatcc aactgcgggt cattgagttg tttccaccac
     4441 tgcgcctgcg gccagccgga gctggcaaga tgaatatcat cggccagttt gatttgttcc
     4501 ggtttgagct gttgatgagg cgcagaatcc ttacgtacca gggcacagcc ggaaatcagc
     4561 gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa gttgacgatt gatcattgcg
     4621 tggctccttg tgcctgttcc gtggcgggtg ttaatgccgg ggctgtccag tctggcaagc
     4681 gggcgaaaag ctggcagacg tgttgctcct gttttaataa ggtgggcagg atggcctgcg
     4741 aggcgggtgt ctcttcaagc gttatttgag gagaacggct gagtgcggtt gccagaccgg
     4801 cagcgtattt ctccagggcg tcggcaaact gacctgctct gtccggctgt agtgcgttat
     4861 ccagcgcctg cgccgagttc caggtggcat cccaggcgtg aagaatatcg cggccctgac
     4921 gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc ttcgctgtcc agttgacgct
     4981 ccagcgccac gcgttggcac atctcttcac aggcattaaa cgccgcatgc agacctatac
     5041 gaatttgcag ataagtgcgc agagccgtga cttcctgctg gcgtgggatc cgcattactt
     5101 tacttagcat acccagcgca ccggccagtt tttgcggcag cgtgcgcgct tcactttcag
     5161 gccagacaaa ggtgtaaatc accgcagaca ccacggtacc aatgatgata cccagggcgc
     5221 gatcgcgaat ttccaccagg tcgtacaccg gaccaaaaac gttttcgagc gtggcgagcg
     5281 cgaaggtgac caccatctgt gtgccgatat aagaagagcg ttcagagctg gtggcaatcc
     5341 atgcgcccaa caggaaaatc ggtgccagca caaacagcaa ttcgacaata ttgtccagcc
     5401 agggcatgac cagtagcgtg aataacagcg ccagaatcgc gccgcaaaag gccccgccaa
     5461 aacgcagcac catcttctgg tacgacgaac cgacatttgg attagcgacg atcacgcagg
     5521 tcagcataca ggtgtgaatg ccttcccagt ccacgccgct gtaaaaggtg taacagatca
     5581 aacaggcgag cagtgttttt accgcgtagc gcatatagtc tggattggta aaagcatcgg
     5641 cggccattga tggcggtttg gctgcgggcg ttggcggcgt attcgggtcc atctgaccca
     5701 gttgtaacag cgtctggcag atattctcca ggttacattc ccgtgccgcc atcgcttcac
     5761 tttcactgat ctgccagtcg ctttgccagc actgaccttc ggtaatggca tactgcagct
     5821 tgttgatttc tgaagctaat ttttgtcgga attcaataat tgcctgagaa tcagcaaaag
     5881 aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt taccgttgcc acgcagcttt
     5941 gccaccatgc actctgggtt cgccagttgg catcgtccgc gaggcaaaag acattgagtt
     6001 tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg tagcggtgcg aggctgtccg
     6061 tcaggtggct aatggcatca tcaagccgat cattaagcac ctgatgcatt tgcgaaatgg
     6121 cacgactggg aaaccacagc acgccgatta acgtcatcag caaggttgga tagaggccaa
     6181 caacgataca ccacagcgtt aagcgcacga ccacttctgg atagtcgagc atggcgggga
     6241 aggtttgccc gtaaatagcg acaatggcga cggcgaaaaa gaccagcccc aaccgatggg
     6301 tgcgcatcaa aaacatgcag cccatcagga tcggtccggc gatgatcaag cggatcaacg
     6361 gttcgccgta tgaccatttg tagatcaaaa acaggctgcc gatctccagc accgtggcaa
     6421 ccacaaacaa gatcgccaca aatttggtgt aaaacgcgtt cgactgaata ccgtaaaaca
     6481 gcactgccag cgataacgcc acgaaaggga tctcaaaggt catcgagatc agaatcacca
     6541 gcaggcagcc tacccagagt tgcacggtct gcggcacgcg gcctggtcgc cgctcgctta
     6601 actcttcatg aaagaacgcc agcagcctga ccaccggtaa tggcagggag ttgagcgcgc
     6661 tcattattgc ggctcaagat tagcgactgc cgaagcgccg atgcggaaca tttccgggtc
     6721 aggtttatca accatgattt tgaccggaaa acgctgggca acgcggaccc agttaataga
     6781 acgagaaact ttcggcaggc cgcccagcac caggccgccc agcaccaggc cgccgtcatc
     6841 cggtagcacg ccgtagccaa ttgaatccac tttaccctcg aaagttttgc cgctgtcact
     6901 catcaggcga atcgttgcgg gtgtacctga gcgaatattt ttcagatcgg tttcgcggaa
     6961 gttggcgatc acgtaccagt gacgagtgtc gattagggta aaaatagggc gcatggcaga
     7021 agcaaattgc ccgacggagg ttttgaggga aatgacccgg ccatcaaacg gcgcgcgaac
     7081 ggtcgccatt tccagatgca gtttggtcag ggcaatatcc gcttcgaccg ccgcacgttg
     7141 cgccaccagc gcatcgacgc cgctgacggc gctggcggct gactgcgcct gtaacaatac
     7201 ggcattaaga tccgcttctg cggcgcgctg cgcagttctt gcacggtcaa cgtcttccgc
     7261 tgagacaaaa ccctctttca gtaatggctc ggtgcggcgt aatgtatctg tggcctgttt
     7321 agcggcggca cgggcttttt ctaccgtggc attaatcgag tcggcaccaa actgttgcgc
     7381 gtcaacgcta cgctgggtga gcataatttg tttatccagc gccgcgaggg aggcttcagc
     7441 tttcgccaga ttggcttcgt acgggcgcgg gtcgatgcgg aacagcaaat cgccctgttt
     7501 caccgcctgg ttgtcggtga ccgccagttc tacaatgcga ccgctgactt ccggcaccac
     7561 atcaatggta tctgctgacg cgtaagcgtc attagttgat ggcgcactgt ctacgcgcca
     7621 gataacgaaa acaagggcaa ccagcgccaa cgcaaccact aacagagcag ggaatttact
     7681 gcgaggagct tttttcggcg tactttccat gagcgcgtct caaatagatt agaaaaatgc
     7741 cagccagaac agcatggcgt agagagcaaa aagggcggta taaataattc cgacaaatgc
     7801 cagattgata tttgtccgct gaataattcg cctcgtgatg agcgtcaaaa taaggctggc
     7861 aatggcgcag aaaaaccagc tgggataata agcgccgatc accgggattg ccggagataa
     7921 tgaacatccc gaaaccatga agacgggaat tatccaggct gtttttttga gttgcatcgc
     7981 catgcgagag agaactgttg gcatattaaa tacatcctgt ctgctgcgaa caatatctgt
     8041 agtgaatatc actatccatt gaaggcgtca gtatggct
//
LOCUS       DAZUCE010000224         8024 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZUCE010000224 DAZUCE010000000
VERSION     DAZUCE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.224"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            3..1169
                     /locus_tag="ACHY03_004217"
     CDS             3..1169
                     /locus_tag="ACHY03_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFS3907389.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEERAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1212..2528
                     /locus_tag="ACHY03_004218"
     CDS             1212..2528
                     /locus_tag="ACHY03_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFS3907390.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYATAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPVGCWLR
                     LSLENDVPEPAHNKKAAASESAFSLLMQHKATIANGILLAIGSTVATYISLFYYGTWA
                     AKYLGMNQNYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            2632..3282
                     /gene="fabR"
                     /locus_tag="ACHY03_004219"
     CDS             2632..3282
                     /gene="fabR"
                     /locus_tag="ACHY03_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFS3907391.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3282..3641
                     /gene="yijD"
                     /locus_tag="ACHY03_004220"
     CDS             3282..3641
                     /gene="yijD"
                     /locus_tag="ACHY03_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFS3907392.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(3681..4781)
                     /gene="trmA"
                     /locus_tag="ACHY03_004221"
     CDS             complement(3681..4781)
                     /gene="trmA"
                     /locus_tag="ACHY03_004221"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFS3907393.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4895..5085
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5150..7033
                     /gene="btuB"
                     /locus_tag="ACHY03_004222"
     CDS             5150..7033
                     /gene="btuB"
                     /locus_tag="ACHY03_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFS3907394.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     gene            6978..7835
                     /gene="murI"
                     /locus_tag="ACHY03_004223"
     CDS             6978..7835
                     /gene="murI"
                     /locus_tag="ACHY03_004223"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFS3907395.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         1864 a         1863 c         2174 g         2123 t
ORIGIN      
        1 cgatgatcga acactatatt cgagggtttg aggaagaact gcgtgagatc cgccatcaga
       61 tccacgaaaa cccggagctg ggattacaag aatttaaaac cagtgctttg gttgcggaaa
      121 agctgcgcca gtggggctat gaagtagagc aaggactggc gacgacgggg atagtggcca
      181 cgttaaaagt gggcgacgga gagaaaagta ttggcctgcg ggcggatatg gatgcgctgc
      241 caatttatga aaatagtggc aagccctggg ccagtaaaca tcctggttta atgcacgcct
      301 gcgggcatga cgggcatacc accatactgc tgggggccgc gcgttatttt gccgaaaccc
      361 gccgttttaa tggcacgtta cgtcttatct tccagcctgc ggaagagatg attaatggcg
      421 gtgagatcat ggttaaagag gggctttttg atcatttccc ctgcgatgtc attttcggca
      481 tgcataacat gcccggtctg ccggtgggta agttttattt ccagccaggg gcactgatgg
      541 cgtcaatgga tcagttccat attacggttc gtggttgtgg cggacacggt gcgatcccgc
      601 acaaagccat cgatccggtg ctggttgccg cacatatcac caccgcatta caaagcattg
      661 tgtcgcgcaa tgtcgatccg ctggaagcgg cggtaattac cgtcggcagc attgttgcgg
      721 gtgaggcggc taacgtcatt cctgacagtg ctgagatgaa aatcagcgtc cgctctctta
      781 gccgcgatac ccgacaactt cttctgaccc gtattcctgc tctggcacaa gcccaggctg
      841 ccagctttgg tgctacggct gaggttacgc atgttaacgg tacaccggta ttagtgaatg
      901 acgaggagat ggcgcgcttc gcctggcagg tggcgtgtaa aacgtttggt gaagagcggg
      961 ccgagtttgg catcaagcct ctgatgggca gtgaagattt ctctttcatg ctggaagccc
     1021 aacccaaagg cggtttcctg ttattcggta atggcgatgt cggggaaggt tcctgcatgg
     1081 tgcataaccc gggttacgac ttcaacgatg caagtctggt cccggcaagc agctactggg
     1141 gcgcactggt agaagcctgg ctgcaataaa taaaacaaca cacaacaaca ctttttggca
     1201 aggaacatgg aatgacggcg atcgtaaatg aaaccgtgag tgtgaagaaa aagcccagcg
     1261 gcaggcgggt gatttttgcc tcggcgtttg gcaatgcgct cgaatttttt gattttggcg
     1321 tttacaactt ttttgtggtc tatatcagca cgctattttt tccgcccagt gcagataaaa
     1381 acgtcgcgct actgctggcg tttgccactt tcggcgtgag cttttttatg cgcccgctcg
     1441 gcggcattat tgtgggggcc tgggccgatc gtttcggacg caaacccgcg atggtgttca
     1501 ccattgcgct gatgagcctc ggtacgctga tgatcggcat cgcgccgaca tatgcgacgg
     1561 cgggctattg gggaaccgcg acgctggtac ttgctcgttt gattcagggt gttgcagcag
     1621 gcggtgaagt tggggcttca atgtcgctac tggtggagtc agctccggca aatcgtcgcg
     1681 gcttttacag cagttggtca ctggcgactc aaggcctggc aacaactttt ggaggtgttg
     1741 tcgctttagg tttaagtgca tggctgccct ttgcgaccgg ttcagaaacc gtcatggctg
     1801 aatggggctg gcgtgtgccg ttttttattg gcgtgttatt agcgcctgtc ggctgttggt
     1861 tgcgtttgag tctggaaaat gatgttcctg agccagcaca taataagaag gccgcagcca
     1921 gcgaaagcgc cttctcctta cttatgcaac ataaagcgac tatcgctaac ggtatcttgc
     1981 tcgctattgg tagtacagtc gcaacttata tctccctctt ttattacggt acatgggcgg
     2041 cgaagtattt aggaatgaac caaaactatt ctcatgcagc gatgttactg gcgggcgtta
     2101 ttacgtttgt gggtgcgctg ctggtgggga tgttgtgcga ttcggtcggg cgtaaaaagc
     2161 tgattttaat ctcccgtgtg atggtgctga tctgtagctg gccgtcattc tggctgttgg
     2221 tgaattaccc aagccctggc atgttgctga cggtggtttt cgtgatggtc agctttacca
     2281 cgcttggcgg tgtgccagtg atgttgttga tctctgaact gttgccgaaa cggattcggg
     2341 cattgggttt tgcgctggtt tatagcattg gtgtggcaat ctttggcggt tttgctcaat
     2401 atttcgctac gcaatccatc gtgttgctgg atagtttgac ggctccggcg tggtatctgg
     2461 gtggaggcac gttgctgtcg atgctggcgt tgttgtacgt aaaagaaccg gccaaagaat
     2521 tgcagtaaat atgttttatt gcgttaccgt tcattcacaa tactggagca atccagtatg
     2581 ttcattctct ggtatagtgc cagcagtact tttggcaagg attcagacat cgtgatgggc
     2641 gtaagagcgc aacaaaaaga aaaaacccgc cgttcgctgg tggaagccgc atttagccaa
     2701 ttaagtgctg aacgcagctt cgccagcctg agtttgcgtg aagtggcgcg tgaagcgggc
     2761 attgctccca cctcttttta tcggcatttc cgcgacgtag acgaactggg tctgaccatg
     2821 gttgatgaga gcggtttaat gctacgccaa ctcatgcgcc aggcgcgtca gcgtatcgcc
     2881 aaaggcggga gtgtgatccg cacctcggtc tccacattta tggagttcat cggtaataat
     2941 cctaacgcct tccggttatt attgcgggaa cgctccggca cctccgctgc gtttcgtgcc
     3001 gccgttgcgc gtgaaattca gcacttcatt gcggaacttg cggactatct ggaactcgaa
     3061 aaccatatgc cgcgtgcgtt tactgaagcg caagccgaag caatggtgac aattgtcttc
     3121 agtgcgggtg ccgaggcgtt ggacgtcggc gtcgaacaac gtcggcaatt agaagagcga
     3181 ctggtactgc aactgcgaat gatttcgaaa ggggcttatt actggtatcg ccgtgaacaa
     3241 gagaaaaccg caattattcc gggaaatgtg aaggacgagt aatgaaacaa gcaaatcaag
     3301 atagaggtac gctgctgctg gcgttggttg ctggcttatc gattaatggt actttcgcag
     3361 cgctgtttag ctccattgtg ccgttttctg tattcccgat tatttccctg gtgctgacgg
     3421 tttactgcct gcatcaacgt tatcttaatc gcaccatgcc ggtaggcttg ccgggtctgg
     3481 cagctgcctg ttttattctc ggcgtactgc tgtacagcac ggtagttcgt gcggaatatc
     3541 cggatatcgg ctctaacttc ttcccggcag tactctccgt cattatggtg ttctggattg
     3601 gcgcgaagat gcgtaaccgt aagcaggaag ttgctgagta atcggtatca tgccggatgc
     3661 tggcgcatcc ggcatgggtt ttacttcgcg gtcagtaata cgccgcactc catatggtgc
     3721 gtgtagggga actgatcaaa cagagccaga cgttcgacct tgtgcgtctg gcttaatgtt
     3781 tccagattct tgcataacgt ttccggattg caggagatat acagaatacg tggatacgcc
     3841 tgcaccattt tctcggtttc actgtccaga ccgctgcgcg gtgggtcgac aaaaatggtt
     3901 tcgcactgat aactctttaa gtcgatccct tgcaggcggt taaactcgcg cacgccattc
     3961 atcgcctgag taaattcttc tgccgccata cgaataattt gtacgttatc aatatggtta
     4021 gcggcaatat tgtattgcgc ggcagcgact gacggcttgg cgatttcggt ggctaatacc
     4081 cgatcaaaat tgcgcgccag ggctaatgag aagttaccgt tgccgcagta cagctccagt
     4141 aaatcgcctt ttgagccttt ggttacgtcc agcgcccatt ccagcatctg aatattcatc
     4201 gccgcgttcg gctgggtaaa gctgttttct acctgacggt agatcatctc tttccctgcg
     4261 accggcagac gttcgtcgat gtaatcttga tccagcgcga ttttggtttt cgttgcccga
     4321 ccaatcagat gcacattcag attctgtgct cgcagtgcat cgcgcagggc ttccgcttcc
     4381 tgacgccact catcatccag ctttttatgg tacagcaggg aaaccaccgc ctgattactc
     4441 agcgtggtga ggtagtcaat ctggaacagc ttgtggcgca gaacgggatt attacgcaca
     4501 cccgcaatca tcgccgtcat caactggttg atgagttcac tggcggcggg gaagctatcc
     4561 acgcggatac ggcttttggt ttgttgatcg aaaatgatgt gatacaggtc atcgccatca
     4621 tgccagatgc ggaactccgc acgcatccgg taatgactga ccggcgagcg aaacacttcc
     4681 ggaaccaggt cagaaaacgg tgccatcata ctttgcaaac gtaccacttt ttcggctaac
     4741 tgcgcttcat actgttctgt tggaaggtgt tcgggggtca tgatgtatcc tgaaaaatta
     4801 aagtacgcgg cgattgtagg gattgctcat cagatgtcca gatcttgatg aattcctatt
     4861 tgtgagctac gtctggacag taacttgtta caacctgtag catccacttg ccggtcctgt
     4921 gagttaatag ggaatccagt gcgaatctgg agctgacgcg cagcggtaag gaaaggtgcg
     4981 atgattgcgt tatgcggaca ctgccattcg gtgggaagtc atcatctctt agtatcttag
     5041 atacccctcc aagcccgaag acctgccggc caacgtcgca tctggttctc atcatcgcgt
     5101 aatattgatg aaacctgcgg catccttctt ctattgtgga tgctttacaa tgattaaaaa
     5161 agcttcgctg ctgacggcgt gttctgtcac agccttttct gcttgggcac aggataccag
     5221 cccggatact ctcgttgtta ctgctaaccg ttttcagcag ccagtaaata ctgtccttgc
     5281 acctactgat attgttaccc gtgaggatat tcaacgctgg cagtcgaaag acctcaacga
     5341 tgttatgagt catctccccg gtgtaaatat ctctcaaact ggtggaatgg ggacgagttc
     5401 ttctctttat gttcgtggca ctgaatcgcg ccatgtttta gtgttgattg acggtgtgcc
     5461 gatggctcgt gccggtatcg acaatgcaat tgatattagc cagttcccgg tatcacttgt
     5521 acagcgtgtt gaatatattc gtgggccgcg ttctgccgtt tatggttctg gtgccatcgg
     5581 aggtgtggtg aacattatta caatgagtaa tgatgagaaa gctcagatca acgcaggaat
     5641 gggtacaaat ggttatcaag cctatgatgg ggtaataaat aaacgttttg gcgatacgat
     5701 ggtaacagca gcaggtgctt accagacgac taaaggtttt aacattcagc ctggctcacc
     5761 atacagtggt gatagcgatc gcgatggata tcgcaacaaa cttttctggg gggggctgca
     5821 gcataagttt aacgataact tctcggggtt cttccgtggt tacagctaca cagctaataa
     5881 tgattacgac cagggctcga tgtatggttc tgctactggc aacgaagaag ctcagaatta
     5941 caaccagtcc tgggatactg gcctacgcta taactccggc atctattcgt ctcagttaat
     6001 tgctaactat caacgactca agaattataa ctacacaaat aatcttggtc gttatgcggg
     6061 agatgccacc ctggatgaca tggaacagcg ttatattcag tggggtaata gcattgaggt
     6121 agggcatggt gcaattagcg gcgggatcga ttggaaacag gaaaaactga cgtccagcag
     6181 cattacaaaa tcagatgatt acgaacgcga taccaccggg ttatacctga ctggacagca
     6241 acagatttcc aatgtgactt tggaagcttc tggccgagaa gatcatgatg aacagtttgg
     6301 ctggcatggt acctggcaga cagctgctgg ctgggagttt attgatggct accaggcaac
     6361 actttcttac ggtaccagtt ttctttcgcc ttctcttgga cagcaatacg gggctgctcg
     6421 ctttgcttca atttatggtc ctggtattgc agccaaccca aatttgaagc cagaagaatc
     6481 taaacagtgg gagattggaa ttgagggggt aactgggact ctggattggc ggctctcagg
     6541 atatcgttat gaaattcaga acctgattga ttataaaagc ataaataatg tcaaccagta
     6601 cattaatgtg aaatcagcca cgattaaagg gctggaatgg acgggaaata ttacaaccgg
     6661 tcctgttgaa catcgcttga cgttacaata tgttgacccg cgcgatgatg aaacagataa
     6721 agtgctgtac cgtcgggcaa aacagcaggt taagtacgag ttgactggac aagcttatgg
     6781 tttcgaatgg gacgtttctt accaatatat aggtcaacga tacgacaacg cttatgacga
     6841 gacctctggc tcatcacata cagttaaatt aggcggtgtg agcttgtggg atcttgcggt
     6901 tgcatatccg gtcacctctc acctgacagt tcgtggtaaa atagccaacc tgttcgacaa
     6961 agattatgag acagtctatg gctaccaaac tgcaggacgg gaatacacct tgtctggcag
     7021 ctacaccttc tgaaccacgt cccaccgtgc tggtgtttga ctccggcgtc ggtgggttgt
     7081 cggtctatga cgagatccgg catctcttac cggatctcca ttacatttat gctttcgata
     7141 acgtcgcttt cccgtatggc gaaaaaagcg aagcgtttat tgttgagcga gtggtggcaa
     7201 ttgtcaccgc ggtgcaagaa cgttatcccc ttgcgctggc tgtggtcgct tgcaacaccg
     7261 ccagtaccgt ttcacttcct gcattacgcg aaaagttcga cttcccggtt gttggtgtcg
     7321 tgccggcgat taaacctgct gcacgcctga cggcaaatgg cattgtcgga ttactggcaa
     7381 cccgcggaac agttaaacgt tcttatactc atgagctgat cgcgcgtttc gctaatgaat
     7441 gccagataga aatgctgggc tcggcagaga tggttgagtt ggctgaagcg aagctgcatg
     7501 gcgaagatgt ttctctggat gcactaaaac gtatcctacg cccgtggtta agaatgaaag
     7561 agccgccaga taccgttgta ttgggttgca cccatttccc tctactacaa gaagaactgt
     7621 tacaagtgct gccagaggga acccggctgg tggattctgg cgcagcgatt gctcgccgaa
     7681 cggcctggtt gttagaacat gaagccccgg atgcaaaatc tgccgatgcg aatattgcct
     7741 tttgtatggc aatgacgcca gaagctgaac aattattgcc cgtcttacag cgttacggct
     7801 tcgaaacgct cgaaaaactg gcagttttag gctgatttgg ttgaatgttg cgcggtcgga
     7861 aaattatttt aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg
     7921 acacggaaca acggcaaaca cgccgccggg tcagcggggt tctcctgaga attccggcag
     7981 agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtat
//
LOCUS       DAZUCE010000225         7925 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZUCE010000225 DAZUCE010000000
VERSION     DAZUCE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.225"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            135..1625
                     /gene="dgcJ"
                     /locus_tag="ACHY03_004224"
     CDS             135..1625
                     /gene="dgcJ"
                     /locus_tag="ACHY03_004224"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFS3907396.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYLDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIASQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1668..2171
                     /gene="yeaK"
                     /locus_tag="ACHY03_004225"
     CDS             1668..2171
                     /gene="yeaK"
                     /locus_tag="ACHY03_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFS3907397.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     INFRRTA"
     gene            complement(2172..2276)
                     /gene="yoaI"
                     /locus_tag="ACHY03_004226"
     CDS             complement(2172..2276)
                     /gene="yoaI"
                     /locus_tag="ACHY03_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFS3907398.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2446..2892
                     /gene="yeaL"
                     /locus_tag="ACHY03_004227"
     CDS             2446..2892
                     /gene="yeaL"
                     /locus_tag="ACHY03_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFS3907399.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2849..3670)
                     /gene="nimR"
                     /locus_tag="ACHY03_004228"
     CDS             complement(2849..3670)
                     /gene="nimR"
                     /locus_tag="ACHY03_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFS3907400.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3767..4948
                     /gene="nimT"
                     /locus_tag="ACHY03_004229"
     CDS             3767..4948
                     /gene="nimT"
                     /locus_tag="ACHY03_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFS3907401.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            5003..5350
                     /gene="yeaO"
                     /locus_tag="ACHY03_004230"
     CDS             5003..5350
                     /gene="yeaO"
                     /locus_tag="ACHY03_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFS3907402.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5372..5626)
                     /gene="yoaF"
                     /locus_tag="ACHY03_004231"
     CDS             complement(5372..5626)
                     /gene="yoaF"
                     /locus_tag="ACHY03_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFS3907403.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5809..6834
                     /gene="dgcP"
                     /locus_tag="ACHY03_004232"
     CDS             5809..6834
                     /gene="dgcP"
                     /locus_tag="ACHY03_004232"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFS3907404.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            6867..6965
                     /gene="yoaK"
                     /locus_tag="ACHY03_004233"
     CDS             6867..6965
                     /gene="yoaK"
                     /locus_tag="ACHY03_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFS3907405.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6968..7042
                     /gene="yoaJ"
                     /locus_tag="ACHY03_004234"
     CDS             6968..7042
                     /gene="yoaJ"
                     /locus_tag="ACHY03_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFS3907406.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(7100..7348)
                     /gene="yeaQ"
                     /locus_tag="ACHY03_004235"
     CDS             complement(7100..7348)
                     /gene="yeaQ"
                     /locus_tag="ACHY03_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFS3907407.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7496..7678)
                     /gene="yoaG"
                     /locus_tag="ACHY03_004236"
     CDS             complement(7496..7678)
                     /gene="yoaG"
                     /locus_tag="ACHY03_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFS3907408.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7682..>7925)
                     /gene="yeaR"
                     /locus_tag="ACHY03_004237"
     CDS             complement(7682..>7925)
                     /gene="yeaR"
                     /locus_tag="ACHY03_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFS3907409.1"
                     /translation="YPRLSVMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWH
                     NIEAMTDDTYFNIDFFVAPEVLMEGAQQRKVIHNGK"
BASE COUNT         1955 a         1950 c         1892 g         2128 t
ORIGIN      
        1 aatttattac tattacccct aaatttcccc tccccagcgg cgcagagatg agtataatta
       61 gcgcccctgt gccaggccgc aatcgaactt tatctggttt tctcgtttca ctaaccgaag
      121 gagtgccatt tatcatgaaa ttgcaccata gaatgctccg gcattttatc gccgcaagtg
      181 tcattgtgct gacatcttcc ttccttattt ttgaacttgt cgccagcgac agagcaatga
      241 gtgcctatct gcgctatatc gtgcagaaag cagactcctc ctttctttat gataagtatc
      301 agaatcagag tattgccgcg catgtgatgc gcgctctcgc tgctgagcag tcggaagtgt
      361 cgccagaaca gcggcgcgcc atctgcgagg cttttgagtc tgccaataat acccatggct
      421 taaacctgac tgcccataaa tacccgggct tacgcggcac actacaaacc gcatccactg
      481 actgcgacac aattgtggaa gctgcagcac tattacccgc ttttgatcag gcagtggaag
      541 gcaaccgcca ccaggatgat tacggttcag gtcttgggat ggccgaagag aaatttcact
      601 attatctcga tctcaatgac cgctatgtct atttttatga gccggttaat gttgaatact
      661 ttgcgatgaa taactggtcc ttcctgcagt caggaagtat tggcatcgat cgcaaagata
      721 ttgaaaaggt atttaccggg cgtaccgtat tatcgagcat ttacctggat cagcgtacta
      781 aacagaacgt gatgagtttg ctgacgccgg tatatgtcgc agggcagcta aaagggattg
      841 tgctgctgga tattaacaaa aacaatctgc ggaatatctt ttacactcat gaccgccctc
      901 tcctctggcg ttttctcaat gtcacgctaa ccgatacaga ttcggggcgc gacattatca
      961 tcaaccagag cgaagataat ctgttccagt atgtcagtta cgtccatgac ttaccgggcg
     1021 gcattcgtgt ctcgttatcc attgatattc tttactttat cacgtcttcg tggaaaagcg
     1081 ttctgttctg gattttgacg gcgttaattt tgctgaatat ggtgcggatg cacttccgct
     1141 tataccaaaa tgtgtcgcga gaaaatatta gtgatgcgat gactggactg tataatcgca
     1201 aaattttaac ccctgaactg gagcagcggt tgcagaaact ggtgcaatcc ggttcttcgg
     1261 tgatgtttat tgcaattgac atggacaagt taaagctaat aaatgacacc ctcggtcatc
     1321 aggaagggga tttagcgatt acgttgttag ctcaggcgat taaacaatcg attcgtaaaa
     1381 gtgattatgc catccgactc ggtggcgatg aattctgcat cattcttgtc gattcgacgc
     1441 cgcaaattgc atcacaactg cctgaacgta tcgaaaaacg tctgcaacat atcgcgccgc
     1501 agaaagagat cggcttctct tccggtattt acgcgatgaa agaaaacgat acgttacatg
     1561 atgcgtataa agcttccgat gagcgtttat atgtcaataa gcagaacaaa aacagccgtt
     1621 catgataacc ttctgtggtt gtttgcttgt aatctcagga gcatgaaatg actgaaatgg
     1681 ctaaaggaag cgtgacgcat cagcggttaa tcgctttatt atcacaagaa ggtgcagact
     1741 tccgcgtcgt tacccatgaa gcggtaggta aatgtgaagc ggtgtcagaa attcgtggca
     1801 ccgctttagg ccagggggct aaggcactgg tatgtaaagt caaaggcaat ggcgtaaatc
     1861 aacatgttct ggcgatcctc gctgcggatc aacaggccga tctgagccaa ctcgccagcc
     1921 atataggcgg attacgcgct tcgttggcca gcccggcgga agtcgatgaa ctcacaggct
     1981 gtgtcttcgg cgcgatcccc cctttcagct tccatccaaa actcaaactg gttgccgacc
     2041 ccctactctt cgaacgattt gatgaaatcg ccttcaatgc aggcatgctg gataaatccg
     2101 ttattttgaa aaccgcggat tatttgcgca ttgcgcaacc agaactgatc aatttccgcc
     2161 gcactgcgta actagcctgt ccgttcaata agcagaacgg acaaaaccag cacaaccgca
     2221 atggcaaaaa acgatgacgt aataatcagt gtctcgacga acatttgatc gttcatagta
     2281 tgccccaggg agatagctgt tttcacgtta ttgttagcgt gcacaaatgg cagtttgatg
     2341 acagttcccc tgatttttta tgcaaaaaac gtaaagattt tctacttcct tcctgcagca
     2401 agcgtaaagt aagcaggctt attatttttt ggcaaggaaa ccacgatgtt tgatgtcact
     2461 ttgctgatcc tgcttgggtt agctgcgctg ggctttatca gtcataacac cactgtcgcc
     2521 gtttcaattc tggtgttaat cattgtccgc gtcacaccgt taagcacctt tttcccgtgg
     2581 attgaaaaac aggggctaag tatcgggata ataattctga cgattggcgt tatggccccc
     2641 atcgccagcg ggacgctacc accctcaacc ctgattcact cgtttctgaa ctggaaatcg
     2701 ctggtggcaa ttgcagtagg cgttattgtc tcctggctgg gtgggcgcgg cgtgacgtta
     2761 atgggcagcc agccgcaact ggtcgccggg ctgctggtag gcactgtttt aggcgtggcg
     2821 ctgtttcgcg gcgtaccggt cggcccgctt attgccgcgg gtctggtttc gctgattgtg
     2881 gggaaacagt agttaatccc gcaatatatc ggcctggcgt ttgccccagg cctttcttaa
     2941 acatagtaat aaaggccgtt gttgaatcat accccagcgt atgagcaact ttctgtacag
     3001 tatcgccttt cactaatccc tgaagcgcca taatcagctg taactgctga cgccactggc
     3061 gaaaactcaa ccccgtctct tttactatca gtcgggcaag attgcgttca ctcatcgcaa
     3121 aaaagccagc ccattgcccc aatgaccccc actcgacggg ccctttcgcc atcatctcca
     3181 ccatcgtgcg gattttagga tgagaagaaa cgggcaggtg cagctgttgt tgtggctgct
     3241 gagggagttc atcaaacagg acctgaatta atctgcgggt catcggctca gcacgctgag
     3301 tcgtcgtcct gtttgccagg gttaaaatca attcccggca taagggcgat attttcaatg
     3361 tgcaacatgt tgtcggcatt gtcactgcgc tgggttcgat aaacagaaaa cagagttcag
     3421 cgttagctgt cacttgattg ctatgttcca ccccgcccgg tatccaaacc gcatattgcg
     3481 gcggcaccat ccacaaagca ttttccaccg tacaggtaat tgcaccatgt agcgccagta
     3541 tcagctgtcc tttgcggtgc tgatgtacag gactggttaa ctcatccgtt ccggcgtgaa
     3601 tacaaaatgc gaccgcggct tcatggtgtc ggtcgggttc atagccattg agattcaacc
     3661 tgtgcatcat tttgtccgaa tttagcgata atttgtcatt ttagcttgat tcaacataac
     3721 aataaaaacg gtaaggtaca gcctcgtttg taacaatgag aagcatatga cctgttcaac
     3781 ttcattaagc ggcaaaaaca ggattgtcct tatcgctggc attctgatga ttgccactac
     3841 attacgcgtc accttcaccg gcgcagcacc gttactggat acgattcgtt ccgcttactc
     3901 gctgacgact gcgcaaaccg gcttattgac caccctgcca ttattggcct ttgcgctaat
     3961 ctcacctctg gctgccccgg tggctcgacg ttttggtatg gaacgtagcc tgtttgccgc
     4021 gttacttttg atctgtgcag gtatcgcaat tcgctctctc ccttcgcctt acttattatt
     4081 tggcggtaca gcggtcattg gcggtgggat tgcattaggt aatgtcttac tgccaggatt
     4141 aattaaacgc gatttccctc attccgtcgc cagacttacc ggcgcatatt ccctgacaat
     4201 gggagctgcg gcggccctgg gatcggctat ggtagtgccg ctggctttga atggttttgg
     4261 ctggcaaggc gcgttgctca tgctgatgtg ttttcctctg ctggctcttt ttttatggct
     4321 gccacagtgg cgaagccaac aacatgcaaa tttgagtacc tcgcgcgcct tacatactcg
     4381 gggtatctgg cgttcgccgc ttgcctggca ggtaacattg tttcttggga tcaactcact
     4441 gatctattac gtaattattg gctggcttcc ggcgatcctc atcagtcacg gctatagcga
     4501 agcacaggcg ggttcactgc atggtttgct gcaactagcc acagcagctc ccggtttgct
     4561 gatcccactt ttcttacatc atgtgaaaga ccagcgtggt attgcagcgt tcgttgcctt
     4621 gatgtgcgca gtaggcgcgg ttgggctctg cttcatgcca gcgcacgcga tcacctggac
     4681 tctgcttttc ggttttggat ccggcgcaac aatgatactg gggttgacgt tcattggtct
     4741 tcgggcaagt tctgcgcatc aggcggcggc actctcgggg atggcacaat ccgtcgggta
     4801 tttgttggca gcctgtgggc cgccgctgat gggtaaaata cacgatgcta acggtgactg
     4861 gtccgtacca ctaatgggtg ttgcaatact ttcactactg atggcgattt tcggactttg
     4921 cgccgggaga gacaaagaaa ttcgctaata tccggtgcta tagtgacgtg acaaatcatg
     4981 cgtgaaaggg agaacaaaca cgatgaatat tcagtgcaaa cgcgtttatg atccggcaga
     5041 acagagcgat ggttatcgca tactggtcga ccgcctctgg ccgcgcggta tcaaaaaaac
     5101 cgatttagcc cttgatgagt gggataaaga aatcacgccg tcaacggaac tgcgcaaagc
     5161 ctttcacggc gaagtcgtcg attttgcaac ctttcgcgag caatatcttg cagaactggc
     5221 acaacacgag caagaaggaa agcggctggc ggacatcgcc aaaaaacagc cgctgaccct
     5281 gctctactca gcaaaaaaca ccacgcagaa ccatgcgctg gtgctggccg actggctacg
     5341 tagcttgtga ttttagtaca gcatccggcg gttatttttc accagccgga tggtcccgtc
     5401 gccacaatgc ccattcatca atcgtttcac cgcccggtaa tttgcaattg ttgctgaccc
     5461 cttgcgctgt ctgcactgga atgagcgtcc cgcccttctg ctggcaatag acagacgccg
     5521 gatttgccat accaatctgc ggcggtttag gtgcttctgg ctgagaaggg gttgaacaac
     5581 cagccaggac cagcaagcaa ggcagaacaa aactgataat tttcatttat tgatctcaca
     5641 tatgtatcca agcttagagt atcgcggtat cgttttgttt tgcagcacta tttttattac
     5701 attcactcaa aacatattac gtcttgtttc atctttgttg atgatgtttt atcatgcctg
     5761 caaagattaa ataatcagca tttacccgcc gtatcctgga gttgttccgt gtcagatcag
     5821 attatcgccc gcgtctcgca atcccttgcc aaagaacagt cactggaaag tctggtccga
     5881 cagcttctgg agatgctgga aatggtcact gatatggaat caacctacct gaccaaagtg
     5941 gatgtcgaag cgcgcctgca gcatattatg tttgcccgta acagccagaa aatgcacatc
     6001 ccggagaatt ttaccgtctc gtgggattac tcgttatgca aacgcgccat tgatgaaaac
     6061 tgctttttca gcgatgaagt ccctgaccgt tggggcgact gtattgcggc acgcaatctt
     6121 ggcatcacca catttctgag cacgccaatt cacttaccgg atggatcatt ctatggcacg
     6181 ctttgcgccg ccagcagtga gaagcgccag tggagtgaac gcgcggaaca ggttttacag
     6241 ttattcgccg gactgattgc acaatatatt caaaaagagg cgttggttga acaactgcgc
     6301 gaagccaatg ccgcgctgat tgcgcaatcg tataccgact cgttaaccgg gctaccgaat
     6361 cggcgggcga tttttgaaaa tctgacgacg ctgttttccc tcgcccggca tcttaaccat
     6421 aagataatga tcgcgtttat cgatctggat aacttcaaat taatcaatga tcgttttggt
     6481 cataatagtg gcgatctgtt tctcattcag gttggcgagc gccttaatac gcttcagcaa
     6541 aatggcgaag ttattggtcg tctcggtggt gatgagtttt tggttgtttc actaaacaac
     6601 gagaatgcgg atatttcgtc gctgcgagaa cgtattcaac agcaaatacg tggagaatat
     6661 cacttaggtg atgttgattt gtattatcct ggtgccagtc ttggcatagt agaagtcgat
     6721 cctgaaacga ccgatgcaga cagtgcccta catgctgccg atatcgcgat gtatcaggag
     6781 aaaaaacaca aacagaaaac accttttgtc gcgcattcag cgccacattc ctgaggcgta
     6841 ttcacatcct tttgattggt gataacatgc gaatcggtat tatttttccg gttgtaatct
     6901 tcattacagc ggtcgtattt ttagcatggt tttttattgg cggctatgct gccccgggag
     6961 cataaagatg aaaaaaacaa cgatcattat gatgggtgtg gcgattattg tcgtactcgg
     7021 cactgagctg ggatggtggt aacgtcacct ctaaaaatag caaaggctgc ctgtgtgcag
     7081 cctttgtgca atttacgcgt taacttttaa tcttcctgta gataaatagc acgacaatcg
     7141 caccaataac ggcaaccacg aagctgccaa aattgaagcc atcgacttta ccaaagccaa
     7201 acagcgtgct gatccaaccg ccgactacgg caccgactat ccccagcagg atagtcataa
     7261 agaatccacc accatcttta cctggcatga tccacttcgc cagaataccg gcaataagcc
     7321 caaaaataat ccatgacaga atgcccattg tttcctcact tatctgtttt gcattagcgg
     7381 gttagtcgct gataaaaagc atagcacaac atcgggaggg caagatttgt gacgagcatc
     7441 acggaggatt tttttgcgat ggcgcagaaa ttgcgccatc aacgatcagt gataattacc
     7501 aaccacaaac atcatgttcg ttttccgtgt cataagaacg cacggtattc accagatctt
     7561 ttatcacttc agccgccact tctggcacca gcaaagtcat cggcgtctct gtttcataat
     7621 cgacagaaac gccattgctg ttattggtga cggtcacggt atacgttgct ttgcccatga
     7681 ttcatttccc gttatgaatg actttccgtt gttgcgcacc ttccatcagg acttcaggag
     7741 ccacgaagaa gtcaatgttg aaataagtat cgtcagtcat ggcttcaatg ttgtgccact
     7801 tttctggagg gaacaccgcg aactgccccg cttcgataag gatcacctga tcaggctctg
     7861 cactgtgttc atcagcgtag ccgagatatt tgaccgcccc atgcataacg gaaaggcgtg
     7921 ggtaa
//
LOCUS       DAZUCE010000226         7748 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZUCE010000226 DAZUCE010000000
VERSION     DAZUCE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.226"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..782
                     /locus_tag="ACHY03_004238"
     CDS             <1..782
                     /locus_tag="ACHY03_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS3907410.1"
                     /translation="SSRFMQSLQQVLNKNEFQLLLGCHEYNQLKETEILMTLLQGNPA
                     ALVIFGSQLADKTHQILEKTNIPTINVVGSPFSPAQITIETAFFEASHKLTEHLLEQG
                     YKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQNTDLVITMPDAASLQLGRYALNEILL
                     RQPELDAVICSHEEIVLGIMFECQRRLLKIPGNIAVACLDGSDNCDQTHPTLTSIRID
                     YKKMGTETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1179..1448
                     /locus_tag="ACHY03_004239"
                     /pseudo
     CDS             1179..1448
                     /locus_tag="ACHY03_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1679..1777)
                     /locus_tag="ACHY03_004240"
                     /pseudo
     CDS             complement(1679..1777)
                     /locus_tag="ACHY03_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase IV"
     regulatory      1898..2086
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2151..3995
                     /gene="btuB"
                     /locus_tag="ACHY03_004241"
     CDS             2151..3995
                     /gene="btuB"
                     /locus_tag="ACHY03_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFS3907411.1"
                     /translation="MIKKYWFLMVYSVTAFSVWAQDTPLDTLVVTANRFQEPLSTVLA
                     PVTIVTREDIDRWQVSSVNDVLRRLPGVAISQHGGEGQLSTIFVRGTNSNHTLVLIDG
                     VRLNLAGVSGAADLSQFPVALVQRIEYIRGPRSAIYGSDAIGGVINIITSRENTGTEI
                     SAGWGSNSYQHYDISTHQQLGENTRVTLLGDYTYTRGFDAVAYGSTGMQPQSDRDGFL
                     SKTFYGKLEHNLSDTWSGFVRGYGYNNRTKYDAWYSPGSPLIDTRKLYSQSWDAGLRY
                     AGETLQSQLVSSYSHSKDYNYDPHYGRYDTSANLDDMKQYNLQWTNSVTVGHGNVGAG
                     IDWQKQSTTPGTGYLPKGYDQRNTGIYLTGLQKLGDFTLEGAVRNDDNSQFERHTTWQ
                     SSAGWEFIEGYRFIASYGTAFKAPNLGQLYGMYGNPNLAPEKSKQWEGAFEGLTGGVN
                     WRISGYRNDISEMINYNPHTLRYYNDGKVHVKGIEATVNFDTGALTHTVSYDYTDARN
                     ALTDEPLERRPKLQVKYQLDWQVFDFDWGITYQYMGSRYDSDYSSWPYKSVKMGGVSL
                     WDVAVAYPVTPHLIVRGKIANLFNKDYETGYGYQAAGREYILSGSYTF"
     gene            4009..4200
                     /locus_tag="ACHY03_004242"
                     /pseudo
     CDS             4009..4200
                     /locus_tag="ACHY03_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4341..6431)
                     /gene="iha"
                     /locus_tag="ACHY03_004243"
     CDS             complement(4341..6431)
                     /gene="iha"
                     /locus_tag="ACHY03_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFS3907412.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITKKN
                     ADKWHSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSL
                     SDTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTRQRYDNQDGQLGSLTGGY
                     DHTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPRE
                     LKESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLH
                     KGISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSY
                     SIDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGTRYRGKTPRFTRNYSSLSAVQKQVYDEKGEYLKAW
                     TVVDAGLSWKVTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            6370..6552
                     /locus_tag="ACHY03_004244"
     CDS             6370..6552
                     /locus_tag="ACHY03_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907413.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTKMRMIY
                     IYLLQKIKIIDKTKYK"
     gene            7211..7711
                     /locus_tag="ACHY03_004245"
                     /pseudo
     CDS             7211..7711
                     /locus_tag="ACHY03_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT         2087 a         1753 c         1774 g         2134 t
ORIGIN      
        1 ccagctcccg ctttatgcag tcgttgcagc aggtgcttaa taaaaatgaa ttccagctac
       61 tgcttgggtg tcatgagtat aatcagctca aagaaactga aattctgatg acgcttcttc
      121 aaggcaaccc ggcggcgtta gttatttttg gttctcagtt ggctgataaa actcaccaga
      181 tactcgagaa aacaaatata cctaccatta acgttgtcgg ttcccctttc agtccggctc
      241 aaattaccat cgaaacagca ttttttgaag catcacataa actgaccgaa catctgctgg
      301 agcagggtta taagaatatc ggatttatcg gtgcgcatat ggacaaccgc ctgcaacgtc
      361 agcaactgaa cggctggcat aaagcaatgc tgagccatta ccagaacact gatcttgtta
      421 tcaccatgcc ggatgccgca agtctgcaat tggggcgtta cgcattaaac gagatattac
      481 tgcgacaacc agagctggat gctgtgattt gcagccacga agagattgtg ctgggtatca
      541 tgtttgagtg ccagcggcgt ttgttaaaaa tccctggaaa tatcgctgta gcttgtcttg
      601 acggttcaga caattgcgat caaacacacc caactttgac atccatccgt attgactata
      661 aaaaaatggg gacagagacg ggtaagctgc tgattggact attgaataat aatcatgatg
      721 aatcagaaga gtcgcgtatc gtgcaattca actaccagat agaattacgt cagagtacat
      781 gagcctaaaa gagcactatt acaaagttag ctattgtggt agggatagcc gtttccggct
      841 attccctgcg cagttccgga tgtatgctgc taacgcatcc attcctactt caggtatttg
      901 gcgtagaagc ggatgctaaa aacttgccgc aaaaccgcaa tatcagcctg cagtatcgct
      961 ctccagatta tccattccta tattgatgac aacgacaggt aagaccgtga cctgataaac
     1021 ctgaataagc tcctggacat aaagtccgta atgggaataa ggcgagagtc agcgaatctc
     1081 atcgaggcca gcagcactgg ctgcaataag gatatagagc agcaatctac cgtcaacgtc
     1141 aaaaacacgg aagccttgca aacgggatgc gttcatataa acaacatgga gcagcaacaa
     1201 tacgatgcct gtcgttgctc agtcaggatc ttttctgtca tgcgcgagcc agcgtcacga
     1261 ttgataaata atgttagcaa ctgattaatt tactgcaaca gacgtggttg gtaataagca
     1321 ctggcggaca atgcaggatg gaaggctgtt ttcaggttgt ccgttcaggt acgttagatc
     1381 agttcgttgc gctaaagaca actgttcagt cccttatgtt acccgttatg gcaaccattg
     1441 cccggtgata accgggcgtg tcatctgtgc atccgttgat tcgtgtggac tgataaagtt
     1501 ttgtctgctg aatctttata cggaatgctc ttcagtatgc gtaagataat ttgttttttg
     1561 gaggattgtc gacaatcaga gggtaaacaa aagaatggtt gaatgtggat gaggttcata
     1621 ttattgattc agattttttg aagaatcacc acatgggatg tccatgtggt tgtataactc
     1681 agggccagcg cttaataatg atggatgtat taatgccgcc aaaggcaaag ttgttactct
     1741 gcaaaaacac acaatcaacc ttgcgggctt catgcaacta attgagcata tatgttttat
     1801 tcttattatg gtcgccgata aaatattatg tgtattagat ccgagtgttc atgcattcct
     1861 atttttgagt catgtatgga cagtaacttg ttacaaccag taatatccag ttgtcggtcc
     1921 tgcgagttaa tagggaatcc agtgcaaatc cgaaactgac acgcagcggt aaggaaaggt
     1981 gcgatgattg cgtaatgtgg acacggtcat ccggtgggtg gtcatctctt tgtatcttca
     2041 gatgcccttc taggtctgaa gacctgtcgg ttaacgtcgc atctggttct catcatcgct
     2101 tactatcgat ggtaactgcg atattcttct tctgttgtgg atgcgttaca atgattaaaa
     2161 aatattggtt tctaatggtg tactccgtca cagccttttc tgtctgggca caggataccc
     2221 ctctggatac tctcgttgtt actgctaacc gttttcagga accattaagt accgtgcttg
     2281 ctcctgtaac tattgtgaca cgagaagata tagatcgctg gcaagtttct tctgtaaatg
     2341 atgttctgcg tcgtttgcct ggcgttgcaa tttctcagca tgggggagaa gggcagctct
     2401 caacaatttt tgttcgtggg acaaactcta accacactct ggtattgatt gatggagtgc
     2461 gcctgaattt agcgggtgtc agtggagctg cagacctgag tcagtttccc gtcgctttag
     2521 tgcagcgtat agaatatatc cgtgggccac gttcagcaat ttatggttca gatgccattg
     2581 gcggagtgat aaatattatt acatcccgtg agaatactgg cactgaaatt tccgcaggat
     2641 ggggaagtaa tagttatcaa cattacgata tatccactca tcagcaactt ggggaaaata
     2701 cccgtgtaac acttcttggc gattatacct atactagggg gtttgatgct gttgcctatg
     2761 gcagtacagg gatgcaacca cagtcagaca gagacggttt tttaagtaaa acattctatg
     2821 gcaaacttga acataactta tctgatacgt ggagcggatt tgttcgtggc tacggctata
     2881 ataaccgaac taaatatgac gcctggtact ctcccggttc tccactgatt gatactcgaa
     2941 aactttatag tcaaagctgg gatgcaggtc ttcgctacgc tggtgaaaca ctgcaatctc
     3001 aacttgtttc cagttatagc catagtaaag attacaacta cgatccacat tatggacgct
     3061 atgacacatc agctaacctg gatgatatga aacaatacaa tctccagtgg acaaatagcg
     3121 tcacagtcgg ccacggtaat gttggcgctg gtatcgactg gcaaaaacaa tccactactc
     3181 ctggaactgg atatctgcca aaaggttacg atcagcgtaa cacggggatt tatctgacag
     3241 gactgcaaaa acttggcgat tttacgttag aaggcgcggt gcgtaatgac gacaactcgc
     3301 aatttgaacg ccacacaaca tggcaatcca gtgcgggatg ggagtttatt gagggttatc
     3361 gctttatcgc ttcctatgga actgctttca aagcgccaaa tctggggcag ctatatggta
     3421 tgtatggtaa tccgaatctg gctccggaaa agagtaaaca gtgggaaggg gcatttgaag
     3481 gtttaactgg gggcgtaaat tggcgtattt caggataccg taatgatatc agtgaaatga
     3541 ttaactataa tcctcatacc cttcgttatt acaacgacgg aaaagtacac gttaaaggaa
     3601 ttgaggctac agtaaacttt gatacaggag cactgacgca tactgttagt tacgattata
     3661 cggatgccag aaatgcctta actgacgaac cattggaacg tcgtccaaaa ctgcaagtga
     3721 agtatcaact tgactggcaa gtattcgatt ttgactgggg gataacttat caatatatgg
     3781 gatcgcgata cgattcggac tactcatcct ggccctataa gtcagtcaaa atgggcggtg
     3841 tgagtttatg ggatgttgcg gttgcgtatc cggtcactcc tcacctgata gttcgcggta
     3901 aaatagctaa tctgttcaac aaagattatg agactggtta tggctatcag gcagcaggac
     3961 gggaatatat attgtctggc agctatactt tctgaaatct gccccaccga gtcgctaaac
     4021 agcgtgatcc gccatgcgat caaaaagcgt aaagtgttcc cgacagacga ggcggtgaaa
     4081 aaagttgtgt ggctggcaat ccagtctgcg tcccagaaat ggacgatgcc gttgaaggac
     4141 tggcgtatgg aaatgagccg ctttattatc aagttcagtg accgcctgga cggtcacttc
     4201 tgggaaaagg catttacaca gaatcctgaa caggctcggt cacttcgacc agacaccgtg
     4261 cgtcatgtaa cgttgccact gttccgccag gagttacagt ggcaacgtat gctaccgtca
     4321 gtgatggcgt tttgttgtta tcagaactga tagttcagcg acatccagta atttcgctca
     4381 ggtatcacat atcctgttgt tgatgatccc gtctggaagt aatcaccggc ataaagcgta
     4441 ctcttaccgg cactgtacag gctcacgtca ctgtaatcct tgttgagcag gttattcacc
     4501 gcagcattca gcgtcagggc atccgtcacc ttccacgaca gacctgcatc caccaccgtc
     4561 caggctttca gatattctcc tttctcatca tacacttgct tctgtacagc gctcagcgac
     4621 gaataattcc gggtgaaacg tggtgttttc ccgcgataac gggttcccag ccatgatgcc
     4681 acctcttcgg tgatctgcca gttcagtttc gcattcacca tgtgttcagg ggtataactc
     4741 agcggtgcac ctttgttatc accatcacgt tgttcacttt gggtccaggt gtaattcagt
     4801 gacagagtga catcctctga ccacagcggc aatgtgccgg cgaattccac accatgcaag
     4861 cgggcctttc cgctgtttgt atagctattg gtgttatcat caatggaata agagacaatc
     4921 ttgttggaga agtctgttat aaagcctgtt atattggcat tcagaccgga gaggttatcg
     4981 taatataccc ccgcctcata acttacactc tcttccggct tcagttcggg gttaccgagt
     5041 agattagtac gtccctgccc ggatacaccg ctgatcccct tatgtaactg tcccatggtg
     5101 ggggctttat atcccgtggt cacaccgcct ttcagcgtcc aggcatcggt cacgtcccag
     5161 accagatatg cacgcggact gaagtgtccc ccgaattggt catgatgttc atagcgactg
     5221 cctgcagtca gcgcaagtac atccgtgaga tgccactcat cctcagcaaa taccgaccag
     5281 cttttctgtc ggaaagtttc acctgtgctg gcaaggacaa ccccatcttt catggacgaa
     5341 ctctgatact ctccccccac agtaagcaga tgagattctc ccagaggggt aagcagcaat
     5401 gaattcagga taaggttaga ttccttaagc tcccgcggtt taccggcaag cccccattta
     5461 tcgctcttca gaacactacg tacaagctca cgacctttat tttctgtctc gttccagttc
     5521 agatacgatt tccatgttcc gaaggtgaaa gtatgatcat agccagctga aattttgttt
     5581 cgctcatagc gcagagtatg gtcatatccc cctgtcaggc tacccagttg accatcctgg
     5641 ttatcgtaac gctgtcgggt ggtatccata tcaaaccaga gcacatcctg ctccgtagcc
     5701 ttccagtcaa tacgcgctcc aagattataa ttctgtgact ccgtgggata aggaatacgc
     5761 gtggctgatg tgtcgctcag tgatgtgacc gatgagccct gacgctgctg tgtgctgccg
     5821 cggatctgca gactgacaga atcatccaca agagggccac tgctccagaa attaaactgg
     5881 ctgctgttgc cccatttgtt actctcctgc agattcagcc cggcgttgac ggaagagtgc
     5941 catttgtctg cattcttttt ggtaatgata ttcaccacac cacccatcgc atcagagcca
     6001 tacagtgtgg acatcggccc cctgataacc tcaatacgct caatcgcggc cagaggaggc
     6061 atgaaccctg tattcatggc agaaaaaccg ttaggcgtca cgtccccgct cccgccctga
     6121 cggacaccat caatcagtat cagcgtgtaa ctggcgggca ttccacggat gctgatttcc
     6181 agcccaccgg ttttacccgt accactttca acatccacac cctctactga tctcagagcc
     6241 tccgccagat cgtggtattg gctggactgc aattcctctt ggctaatcac agaaacactg
     6301 gcggctgcgt tagtcagctt tttctcatag ccggatgccg agacaatcat cacatcctct
     6361 gcagcagcta tgctgctggc tgaaaatccg agacagggaa tgactacgga agccagagtg
     6421 gttattcgca tattactaat ctccgatcat gttaaccggc aagacaatac acaaatacaa
     6481 acaaaaatga gaatgattta tatttacctt ttacaaaaaa taaagattat tgacaaaacc
     6541 aaatataaat aacgactgcc tcggcatgcc agttaacagc tatccgtatg gcgtgttcgg
     6601 ttttgcgagt aaaaaatggt tactgtagaa gcggatttcc accacatgtc aaccatgaat
     6661 ggtcagtggt gcgagacgac tgggatgatt tggtcgatga cttcttccgg cgaatactga
     6721 tagaaacatt gaaatattgt aagagaaagc gttcaaaatt ctgtggcacg tgaaaaattc
     6781 tacagcgtca tcaccttatt cagtcgacct tcaaaataaa cgtttaacag agacaatcac
     6841 catttggatt gtccgctgag gaggcgaacg atttcctaga cagctttatc atgtcagccc
     6901 acattgttgg cgctgacaat actaaggaca aacgatgggg aagtagcttt ttagcgttat
     6961 tttcgggagt aaaaaacgtg gtatctttcc gtatgtcagt tgtatcttgc tggtattcgt
     7021 agtcgtgata gggcatacaa cgatatttat cttgacgtta gtgaagaata agttgttttg
     7081 tgatctattt taatctggga ttacaatccg ggcttgatac atcgcatcac gacttttatt
     7141 ctcttcagaa tagaatatgt tactctccgt tttacaggct caggatttga attacaaatt
     7201 atacttttaa gtgaacaata accaggggta tttcttattt cctgaaacct ggtttggccc
     7261 atttcgggat gagtttgaaa aaactcttga cgcatacgat agtgatgaaa taaacgaaac
     7321 aaattacgtc aacaaactca ggcgtcttgc ctgacatgct cctgacatca ttgatattca
     7381 cactcatctg gcatatgcat ttgcgctctt tatgctgcaa ttctggcgaa tgttcattta
     7441 cagcgtcatc aggatgccgt tatactcatt gataaaatac taacatataa ccctgagaat
     7501 aatcaggggc cacgctggtt actgggggcg gaattgttac gtgctggcaa gcatgacagg
     7561 gctctgtacg tattgacagg acatgtcaat gaattttccc cctactggta cgagcttgct
     7621 cttttacatt ttatgaatga ggcatatatg aatgctgcaa cggcatttcg atatggattt
     7681 ttaacaagca tatattgttg aaatgctttg ttagaatctt catccattcc ctggggctgt
     7741 atagtatg
//
LOCUS       DAZUCE010000227         7746 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZUCE010000227 DAZUCE010000000
VERSION     DAZUCE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.227"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..417)
                     /locus_tag="ACHY03_004246"
     CDS             complement(<1..417)
                     /locus_tag="ACHY03_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS3907414.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQ"
     gene            complement(417..758)
                     /locus_tag="ACHY03_004247"
     CDS             complement(417..758)
                     /locus_tag="ACHY03_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3907415.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVN"
     gene            complement(751..3993)
                     /locus_tag="ACHY03_004248"
     CDS             complement(751..3993)
                     /locus_tag="ACHY03_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS3907416.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVADGITKVENAFMAWVGGANEASG
                     ATKTLSGVLNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIVTARARAAVYRAQQAVAAARGTEMQIAAEARLAVTQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESAHQYALT
                     IDEIAHKTPSMSLPEASDNEGRTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEELAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLVMNGQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLD
                     DKQQGALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSGAEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREEALKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGRMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(4042..4257)
                     /locus_tag="ACHY03_004249"
     CDS             complement(4042..4257)
                     /locus_tag="ACHY03_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFS3907417.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDEDKPEVDPFAALEDALSLAAQS"
     gene            complement(4353..4727)
                     /locus_tag="ACHY03_004250"
     CDS             complement(4353..4727)
                     /locus_tag="ACHY03_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFS3907418.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QDVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDILCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(4742..5458)
                     /locus_tag="ACHY03_004251"
     CDS             complement(4742..5458)
                     /locus_tag="ACHY03_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFS3907419.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(5524..5868)
                     /locus_tag="ACHY03_004252"
     CDS             complement(5524..5868)
                     /locus_tag="ACHY03_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFS3907420.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(5865..6311)
                     /locus_tag="ACHY03_004253"
     CDS             complement(5865..6311)
                     /locus_tag="ACHY03_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFS3907421.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGTENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(6308..6658)
                     /locus_tag="ACHY03_004254"
     CDS             complement(6308..6658)
                     /locus_tag="ACHY03_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFS3907422.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(6668..6994)
                     /locus_tag="ACHY03_004255"
     CDS             complement(6668..6994)
                     /locus_tag="ACHY03_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFS3907423.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMEKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(6991..>7746)
                     /locus_tag="ACHY03_004256"
     CDS             complement(6991..>7746)
                     /locus_tag="ACHY03_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFS3907424.1"
                     /translation="DGKSLTPDDVRPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVN
                     DAVANIPQPADGKSITPDDVRPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVLDAVS
                     ALPAPQDGRDATALEILPAIDDQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGV
                     ADIDVSMTGERLFSVVVRQSSGQRTAKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSL
                     WHCNSMTGDKPGEAHSSAWTLAAKRGRDAGGGK"
BASE COUNT         1569 a         2614 c         1824 g         1739 t
ORIGIN      
        1 ctgctccacc acccaccggt ccgtcagctc ctgctccggg tcggcctcag gattgcctgc
       61 cacaaaattc accgcatcca gaaaacgcgc atacacccgg cggcggacca ccgtggcccc
      121 caccaggctc tgcaaatcct ccgccatccc ggtgacaagg ccgaaaagat tggacactgt
      181 cagcgacggg cgggcactgc tgccctttcc gttcatctca aagccgctgc cctcaatcgg
      241 gtacgcctga tattcacgcc cctgccaggt caccggctcc cctttttcat tcagctcatt
      301 gcagaaaaaa taccgctcac cgccctgcac cgtcaggtcg atttcccaga gcaccacccg
      361 cggtgactgc tctgacttaa ccgactcgtt caggctttct tcgtgaatat cctgcatcag
      421 ttcaccacct gctctatcgt gcaactgaaa tcactgtacc gggcgttatc cgtaatactc
      481 cactcccggc acaccactct gaccgtccgg ttatgtttcg ggggcttcca caaaaaagcc
      541 cggtaaccgc catgccatga cagaaacgcg gacagtgcct cccgctcagc atccgttgtc
      601 acccgaaaaa ccacctgaaa ggtcttcagc tgcgcattca gtcctgccgg acgacgctgc
      661 tgataaccgt cgccaaactt caccgtcacc accgacggtt tctccgtcac ctgcatccct
      721 tcccggggac accagtgaag ggtcttaatc tcatccactc agcattcctc cgtcacgacg
      781 catggataac atcaccgcct gtaccctctg gtctatcagt tgcacaaggg tgcccgccgc
      841 ttccggccct atctgcccgt tagcgccgtc attctgaatg gcgatgtggt acaccgggga
      901 atacaccaga cccgcactgc cgttcatgcg gcccaccgca cgcacgccga gtgaaccatc
      961 cgctgcccgg gtcaggggca tgatggcttc aggtccggcc tcccccatca gccctgcccc
     1021 ttttgcaaag gcaaagtacg tgggcgtgtc cacaatactg ttactgtatg cactcaggtt
     1081 tgccgaggta taaacgccgc cttttgcatt tgccaccgcg ccacccagcc agttgcctat
     1141 actgccgaaa aatcctcctg caccggacat actgtttgcc gccatcttaa tgccgttgac
     1201 aatggccgca ttcataagaa cttttgatat ttcctgcagg attgatgcag cccagctgcg
     1261 ccattccact ttatttccgt tcagcatctc cgtgatgtta ttcaccatcc ctgagatacc
     1321 gtctgtcgca agctgtgctg cctgtgaagc gtaatctgat gcgctgtcca cccagttact
     1381 tagcccttcc tgcagccctt tctgccagtc agcacgctgc gcatccgatt cggcataaaa
     1441 agcctcctgg tctttaaggc gttcactcag atactgcgca ttctgcgcca gcgcctgtct
     1501 gtaaaaatcc tcactgatat cccctgtctg atactgagac tgcaggtccg catccttctg
     1561 gcggtagccg tcacgaatct gctgcagctc ccgcatccgt tcacggaccc gctcaccctg
     1621 cccgtacccc agcagctcag cgtcatttga tgccctttcg tctgcattat catttttcag
     1681 agcctcctcc cgggcccgta actgctcccg gattttcttc tggtcaatca gggcagcatt
     1741 gcgcagcagc tcctgcttct gtatctccgt cagggttttc agctcgccct gcgaggtctg
     1801 gtatttcagt tttgccagtt ctgtattctt cccggccagt gccagttgtt ccttctgctg
     1861 cttcagcaga cgggaaaaac tgtcttccgc tttttccgcc cctgattttc cggcccggga
     1921 tttgggttta ttcgcctcat tattgcgcca ggcttccatc gaattacgga tgtaacgctg
     1981 cctcgcctcc tgatacgtat cccccaccag accaaggtca tccgccgcat accccagccg
     2041 ggcacgctct ttttcttccc ctttcagccg ggacagggca agttgacgct ctgtgttatt
     2101 cagggcgccc tgctgtttat catccagagt ggcctgtggc agtcgcagcg gtacactcgc
     2161 cagtccctga cgctgttgca gcagttcatt acccagcccc agcaggcggt tgaattccgt
     2221 atgctgaccg ttcatgacca gcagtgactg atacgcttta ttctgctctg ctgcctgctg
     2281 acgaattaac acaacacggc gctcttccag tccggcaagc acatcctgaa tggactgcgc
     2341 tttttcctgc atctgtgcca gacgggactg ctcaacagca aactgctctg ttgcctgagc
     2401 aagctcttcc gttacagttt tcaccgatgt cagatggttt atcatgaagc cgttaccggt
     2461 cgtccagccc ggattagcca gcacatactg atacccggcg attttttcct gcagggattt
     2521 cacccggctg gcctgctcat caatcagccg gttctgctct gtcagcgcct cccgtgttcg
     2581 cccttcatta tctgaggctt caggcagaga cattgatggc gttttatgcg cgatttcatc
     2641 aatcgtcagt gcatactggt gcgcagactc cctggcctgt tcctgattct ggtacagcgt
     2701 gtaccatgcg gcagctccca gcatcaccag tccgggtacg cctccaacca gtcccagcgc
     2761 accgctcatc agacgtgagc ccaccgccgt tgtactgttc agcgcattct gggcggcggt
     2821 tctggcagca atatttctgt tcaggcgttc ctgtgtgacc gccagacggg cctctgccgc
     2881 aatctgcatc tcagtcccgc gggctgccgc cacagcctgc tgagcccggt acacggctgc
     2941 ccttgcccgc gccgtgacaa tctgcgttcc ccttaactgg gcctcagcca gtgccacttc
     3001 attacgtgca gcggtcacaa gtcctgccgt ggcagacatc gctccggaag ccagattacc
     3061 aaagtaccgg gccaccccga cagcaaccag cacacccgca gctgttgcca cggtatcaat
     3121 atttccggcc acaccgttca gcacgccgga gagcgttttc gtcgctccgc tggcctcatt
     3181 cgcgcccccc acccaggcca taaaggcgtt ttccaccttc gtgatcccgt cagccaccgt
     3241 ttccggcatg gctgcatatt catcacgcaa tatccccagc tggctgatta acgcgggaac
     3301 gactttatcc gccgtcagtt gaccatcatc cgccatcgcc ttcagatctt tacgggccac
     3361 gcccatgcct gcagccagtg cgcgaatgat ccggtcccca ctttcattga ccgaattaaa
     3421 ttcctcaccg cgtaacacac cctgtgccag cgcctggctg aactgggtga tcaccgaacc
     3481 cgcctctgcc gtactggcac cggaaatttt cagccctgtc gaaatggcct ccgtcacctt
     3541 cagcacatca tcagcactgt aaccatattc acgcatcgag gcagccgaac gggcaaacag
     3601 agccgcatta tccgaaaatg cagtgcctgt ccgctggctg atgtccatca gcactttctg
     3661 tgatgacgca aattcatccg atgactgcga cgcctgtttc agacgggcat tcacggagct
     3721 ccattcatcc gccagcgaaa tcaggtgtcc ggtggcaaag gcaccggcaa acgcaccggt
     3781 cattccgaca gccgaagcgc ggatttccgt caactggctg ttcagctcag ccagagcccg
     3841 gcgctgctcc ctggctgccg cagcagcctg acgtccgcca ttctgcaggg tccggtaata
     3901 ttcactgccc atacgggacg cccgctggat ctccgactga aatgactgtg aatttgccga
     3961 aattttgata atcagttcac gtaacgtcgc cattcacctt tctccgggcg taaaaaaaac
     4021 cgcctcagcg gttctcatca ttcatgactg tgctgcaagg ctcagcgcgt cttccagcgc
     4081 cgcaaacgga tccacctccg gcttatcctc atcctcatcc tcgccccagc agagcatggc
     4141 gtccttcagt gcaacattca tcccctgcgc cccgaaaacc gctttcacga tctgtgcatt
     4201 acggatatcc ccgcgctcat cacccagcgg ggatatcctg tcgaactcca tccacatcat
     4261 cgcctcgctc gcactcaggc tgtggcgcag ttcggataag gtgcgcccca gacggagcgc
     4321 aagtcgcatc agaaagcgaa tttccgggcg ggctactttt ttctggccga ctctgcatca
     4381 gcgatcagtt ccagtgcctg acgcagcaag cgggcatgta ccggaccata gacggccagc
     4441 acctgctcac ggtcgtccgg agtgaacacc cgctgcaggt ccgtatcaca caggatatcg
     4501 cagaacagcg tcacatccgc ttccaggtta cggcgggttt tcgccaccac cgacagggta
     4561 tcgtcatcct ctccatcacc attgagcacg tcctgccaca gataccaggc ctctgccgaa
     4621 ggctcccgca gcaccacgct gacattaccc cattccggca ccttcaccgt tttatgacga
     4681 aaccctgaca gtctggccag cgccagcgtt ttcagatcct ttttcatgat gccccatccc
     4741 cttatccggc ggctgcgctc actgtcacgg tgcattcaac agacgtcaca ctctgtgctt
     4801 tctctgccga atcggtcacc acgcaggtat atttccccgc atcagcggac tgcgcacctg
     4861 gcttactgaa ggtgtctgtc gtctgcccgt caaccggctg accatccttc ttccaggcgt
     4921 atttatacgg cggcgttccc ccgttggcac tgactgacat tgtcagcagc gcaccggtat
     4981 tcacggtaag tgtcttatcc agatttttca caaacgccag cggtaccaca aaggacaccg
     5041 gtttgccttt cagacgcagt gagaacgttg cagccaccac gccgttggta ccggatgacc
     5101 aggtgtgctg acgcacttcc gccaggaatt taaagccctt accggacgga aacagcacct
     5161 taaacgcata caacgcgtca ttgtcatagg catcacgcag ggcgttctgg gcctgattca
     5221 gataaaaatt acccgacatg gaaatctcag acgacgcccc cagaccattg atgttctcct
     5281 gctctgtgga gcagagcgtg gtcacatcaa tatcctgttt ctgaccggcg gtgaactgga
     5341 cttccttgat ggtgcagtcc aggcgcagat attccgcctt atccatagtt tcagcagtcg
     5401 ccggggcaga tgaaatcatc acctgcgtca gctgtgagcg ttcatacaaa gcagacattc
     5461 tgcctcctga taataaaaaa cccgcacgcg gcggggtatg ggttttgtag aaaaaagaaa
     5521 aagtcacacc gtgacctgaa actccagggt tgcacggtaa cagcggtttt ccggaatata
     5581 gtcctgcatt tcactgacgg atcccggggc cagcagcatt atggcttcac gggcgtcctg
     5641 acgtatctga cgcgcctgcg tcacagtccc ggcataaacg tctatctgca ccgacactga
     5701 ggactccgcc tgcccgccca tcacgtccgc tgacaccgat gaaatcaggc tgaaaaccac
     5761 ccacggaagc gccaccgacg gcctgccatc cagcaggggg accacatacg ggtacacctg
     5821 cccgccggca agatgcgcca gatgaggata caaatccgcc tccgtcatcg tctcagtacc
     5881 tcatcaatgg cccggttcat ccgcgcaatc gccacctgcg ctgcctgttc actgcgcaca
     5941 tcaaacgccg ggcgcacaaa cgggtgcgga ggcatattca cggtccccat ttccacaaac
     6001 cgccagtaga aagcattgcg cgggttatcc gccttcatgg tgttatcgct gttaccggtg
     6061 tccggattaa caccacggat atggacaccg gattccatcc cgccatcgcg ggagcgccgg
     6121 gaaaggacca ccacattgcg gcgcagtttt cccctgcgta ccggtgcccg tgacaccact
     6181 tcttctttca gcacattcgc ccccgcacgg gttgcctcac gcagcacccg gttgttttcc
     6241 gtaccactca gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa atccagcagg
     6301 gtttcgatca tttttcccct cccagccgac agagaatttc cagacgtccg ccggtcgcat
     6361 ccggcacggg cagcccgaca acgttcagga tccggtcacg ccagggacca ctcagcacat
     6421 gaagtcgtga cgctgccgtg atttcccgac cggactgacc gcgcacccag atgcggattt
     6481 ccgcctgcgc catttccgca ccggactgca tccgctcccg gctgctcctg ccacggatat
     6541 ccgcatgaat tttcccgcat gacacccatt cttccgtcat ttctccggca gcattacggg
     6601 ttaacaccgg gttcagaaca cttatcatct gtgtcagacg acctgcagat attgccattc
     6661 cccctcctca taacaccgtc ggacaacgca aatcgtaaat cagcacggaa acagaaaacg
     6721 gcagctcccc ctgaagcagt tcttcccgct ccgcaagatc cggattccgg tacagcatcc
     6781 cggtcagtcg catggcagcc cccttcatcc gggttaatgc ctcgcccggg atcagttcac
     6841 cgtcctcacg aatcacttta tcccggctgc cctgaatgta ggccagcagc acggcggtag
     6901 cctgacgaac cttctccatc agcatgtcat catccgcgtc atggtcgaca cgcagatgtg
     6961 ccttgatctc ttccagtgtc agtaatgccg tcattttccg cctcctgcat cccgcccacg
     7021 ttttgcagcc agggtccagg ctgatgaatg agcttctccg ggtttatcac cggtcatact
     7081 gttgcagtgc cacagcgagc ccccccatgt caccgtatcg ccggggtggt aggtttcacc
     7141 ggctctgaac acaccgcggt agagcatcac cggcagggaa aatgtttttg ccgtacgctg
     7201 gccactgctc tgccggacca ccacagagaa caaccgttca cccgtcatgc tgacgtcaat
     7261 atccgccacc ccgtcaacca ggcattccca tccccgcatc ccgtgcgttt tttcatacgc
     7321 ccgccagagt ccgccctggt gtgtggcata cgtgccccgg ggaaaggatt tttgatcgtc
     7381 aatggcaggg agtatttcca gtgccgtggc atcacgcccg tcctgcggag ccggcagggc
     7441 actcaccgca tccagaaccg ccttctgcag aacatccgga tcgtagtcac gaccatcacg
     7501 cggaacagga atatggctca ctgcctcctt caccatctgt tcaagcatcg gacgcacatc
     7561 atccggagtg atacttttac cgtccgccgg ctgcggaata tttgcgaccg catcattcac
     7621 cgccttctgc agaacatcgg gatcatagtc acgaccatca cgcggaacag gaatatggct
     7681 cactgcctct ttcaccatct gttcaagcat cggacgcaca tcatccgggg tgagactttt
     7741 accgtc
//
LOCUS       DAZUCE010000228         7712 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZUCE010000228 DAZUCE010000000
VERSION     DAZUCE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.228"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(183..464)
                     /locus_tag="ACHY03_004257"
     CDS             complement(183..464)
                     /locus_tag="ACHY03_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFS3907425.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            893..2479
                     /gene="pdeC"
                     /locus_tag="ACHY03_004258"
     CDS             893..2479
                     /gene="pdeC"
                     /locus_tag="ACHY03_004258"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFS3907426.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCSPAHQKRMLNIIRGYLYINELIYARDN
                     HFVCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKCKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(2482..2805)
                     /gene="soxS"
                     /locus_tag="ACHY03_004259"
     CDS             complement(2482..2805)
                     /gene="soxS"
                     /locus_tag="ACHY03_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFS3907427.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            2891..3355
                     /gene="soxR"
                     /locus_tag="ACHY03_004260"
     CDS             2891..3355
                     /gene="soxR"
                     /locus_tag="ACHY03_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFS3907428.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSVRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            3901..5250
                     /gene="ghxP"
                     /locus_tag="ACHY03_004261"
                     /gene_synonym="yjcD"
     CDS             3901..5250
                     /gene="ghxP"
                     /locus_tag="ACHY03_004261"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFS3907429.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            5401..7050
                     /locus_tag="ACHY03_004262"
     CDS             5401..7050
                     /locus_tag="ACHY03_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFS3907430.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(7204..>7712)
                     /locus_tag="ACHY03_004263"
     CDS             complement(7204..>7712)
                     /locus_tag="ACHY03_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS3907431.1"
                     /translation="IAPYINLFRADLSFSKLDLINFEHADLSRVNLNKATLQNINLID
                     SKLFFTRLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSVLTKAWMFNIRLYRVNF
                     DEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNIHAVAMKIT
                     ADIMQHAD"
BASE COUNT         1806 a         1763 c         2020 g         2123 t
ORIGIN      
        1 tgaactatca aaaattgctc atttgtataa aattcagcaa gggggtggcg aagatagtga
       61 agatgctaag cgtacaacac cttcaagcgt tgattaaaag aaagtgcgat atgtgatgat
      121 agggcggccg aggaagctat tcatcgaccg cttgtgaagg gataaaagtc aagaaagtga
      181 aatcaggtca aaatcattgg ccattgtggc ggtgtctgat tcatctcttc tatcatcacc
      241 gactcgatgt tgtgccagat agcggtgatg tccgccattg tgatgccaag caaccccata
      301 gcgaaccaac aggcggaaac cacgcccaga ccgctgctga tcactgcaag tccgacataa
      361 tcagaccgac gaaaacggat gttgagcgtg ctggccagaa acatcattac ggcactaaga
      421 agttgccagc gaagaagaac cacgccagtg gtgagggtag ccatcaaaca attcctctga
      481 aaagagccga tgccctggac agcgcggcgt tgttcacggg aggcgtggta cactctggct
      541 atcgcggggc ttgcagaaca caaaaatgaa acactctgtt tgtttcatta attttgtgaa
      601 ctatatcaca attgattgtt tgttagccat attaggacgt gacttttatt gctgtacaga
      661 ttatgtggtt tttcagtggc attaagggta tatcttcccg ccgcctctgt attcctgtag
      721 aaaattaatt ttgaatatca atgaattatt ttcatccagg tgacgattac aaaggtatca
      781 atttcaaatc aggcaaaagt gctatttata ccgtaagatt tatctaaaga cgtcggtagc
      841 cagggtttca ccttgcaatg gccgggtata aacaggcagg aaattgatag caatgagtca
      901 tcgtgcacga caccaattac tggcgttgcc gggcattatc tttttagttc tctttcccat
      961 cattctttcg ctatggattg ccttcctttg ggcaaaatca gaagtgaata atcagctccg
     1021 aacctttgct caactggcac tggataaatc cgagctggtc attcgccagg cagatttagt
     1081 gagcgatgca gcagaacgct atcaggggca agtttgctct ccagcccatc aaaagcgaat
     1141 gttaaatatt attcgtggct atctttatat taatgaattg atctatgccc gtgataacca
     1201 ttttgtatgc tcatcactga tagcgcctgt aaacggctat acgattgcac cggccgatta
     1261 taagcgtgaa cctaacgttt ctatctatta ttaccgcgat acgccttttt tctctggcta
     1321 taaaatgacc tatatgcagc ggggaaatta tgtggcggtt atcaaccctc tcttctggag
     1381 tgaagtgatg tctgatgacc cgacattgca atggggtgtg tatgatacgg tgacgaaaac
     1441 ctttttctcg ttaagcaacg aggcctcggc agcaacgttt tctccgctga ttcatttgaa
     1501 ggatttaacc gtacaaagaa atggctattt atatgcgaca gtttattcga caaaacgccc
     1561 aattgcagcc attgttgcga cttcatatca acgtcttata gcccattttt ataatcatct
     1621 tatttttgcg ttacccgccg gtattttggg gagtcttgtt ctgctattac tctggctacg
     1681 tattcgacaa aactatttgt ctcccaaatg caaattgcaa cgcgccctcg aaaaacatca
     1741 actttgcctt tattaccagc caataatcga tatcaaaaca gaaaaatgta tcggtgctga
     1801 agcgttatta cgttggcctg gtgagcaggg gcaagtaatg aatccggcag agtttattcc
     1861 gctggcagaa aaggagggga tgatcgaaca ggtaactgat tatgttattg ataatgtctt
     1921 ccgcgatctg ggcgcgtact tggcaacaca tgccgatcgc tatgtttcta ttaacctgtc
     1981 ggcctccgat tttcatacgt cacggttgat agcgcgaacc aatcagaaaa cagagcaata
     2041 cgcggtgcgt ccacagcaaa ttaaatttga agtgactgag catgcatttc ttgatgtcga
     2101 aaaaatgacg ccgattattc tggctttccg ccaggccggt tacgaagtgg caattgatga
     2161 ttttggtatt ggctactcta acttgcataa ccttaaatca ttgaatgtcg atattttgaa
     2221 aatcgataaa tcgtttgttg agacgctgac cacccataaa accagtcatt tgattgctga
     2281 acatatcatc gagctggcgc acagcctggg gttaaaaacg atcgctgaag gcgtcgaaac
     2341 tgaggagcag gttaactggc tgcgcaaacg cggtgtgcgc tattgccagg gatggttctt
     2401 tgcgaaggcg atgccgccgc aggtgtttat gcaatggatg gagcaattac ccgcgcggga
     2461 gttaacgcgc gggcaataaa actacaggcg gtggcgataa tcgctgggag tgcgatcaaa
     2521 ctgccgacgg aaaacgcggg agaaggtctg ctgcgagaca taacccaggt ccattgcgat
     2581 atcaaaaatc ggacgctcgg tggtgcgcaa ctcaacggcg gccagtaaca ggcggcgttg
     2641 gcgaatgtaa tcgccaagcg tctgatgcgt caccgtgcgg aacatccgtt gcaggtacca
     2701 ctttgaatag cctgattttt ttgcgacgac atcaatgtta agcggctggt caatatgttc
     2761 gtcaatccat gcaataagat cctgaataat tttctgatgg gacataaatc tgcctctttt
     2821 cagtgttcag ttcgttaatt catctgttgg ggagtataat tcctcaagtt aacttgaggt
     2881 aaagcgattt atggaaaaga aattaccccg cattaaagcg ctgctaaccc ccggcgaagt
     2941 ggcgaaacgc agcggtgtgg cggtatcggc gctgcatttc tatgaaagca aagggttgat
     3001 taccagtgtc cgtaatagcg gcaatcagcg ccgatataaa cgtgatgtgt tgcgatatgt
     3061 cgcaattatc aaaattgctc agcgtattgg cattccgctg gcgaccattg gtgaagcgtt
     3121 tggcgtgttg cccgaagggc atacgttaag tgcgaaagag tggaaacagc tttcgtccca
     3181 atggcgagaa gagttggatc ggcgcattca taccttagtg gcgctgcgtg acgaactgga
     3241 tggatgtatt ggttgtggct gcctttcgcg cagtgattgc ccgttgcgta acccgggcga
     3301 ccgcttagga gaagaaggta ccggcgcacg cttactggaa gatgaacaaa actaaagcgc
     3361 cacaagggcg ctttagtttg ttttccggtc tttgtctttc tctctatccc gctggtacac
     3421 aggagggttt cccccgacgt caacacacct cattcgagca cgtggtggag gttccggttg
     3481 gtgttgatgc tttaattgta tgtcaccgac gtttcttcgc cagtgtaaaa gtatactttt
     3541 taaccgcaat atttttgtca tctcagacga ttttttatcg caatcctgaa cggtatacgg
     3601 ctcgataacg ctgcaatctt gcgcaccgac gataacgttt gcgcatcaat tgcctggttt
     3661 ttcatcgtca agacaataaa agagaaaaaa gcagcaaact tcggttgaaa aagccgctat
     3721 gatcgccgga taatcgtttg ctttttttac cacccgtttt gtatgcgcgg agctaaacgt
     3781 ttgctttttt gcgacgcagc aaattgtcgc aaacctggag caggaagata acgtttcgct
     3841 ggcaggggat tgtccgccac gcatcttgac gaaaattaaa ttctcagggg atgttttctt
     3901 atgtctacgc catcagcgcg taccggcggt tcactcgacg cctggtttaa aatttcacaa
     3961 cgtggaagca ctgtccgtca ggaagtggtt gccgggttaa caacgtttct ggcgatggtc
     4021 tactcggtta tcgtcgttcc aggcatgttg ggtaaagcgg gcttcccgcc tgcggcagtt
     4081 ttcgttgcaa cctgtctggt tgccggactc ggttctatcg tgatgggtct gtgggctaat
     4141 ctgccgttgg cgattggttg cgccatctcc ctgacggcgt ttaccgcatt cagcctggtg
     4201 ctggggcaac atattagcgt acctgtcgcg ctgggtgccg tgttcctgat gggtgtgctg
     4261 tttacggtca tttctgccac gggtatccgt agctggattt tgcgcaactt gccgcacggt
     4321 gtggcgcacg gcacggggat cggtatcggt ctgttcctgc tgctcattgc cgctaatggt
     4381 gtcggtctgg tgattaaaaa cccgcttgat ggtctgcccg ttgcgctggg tgatttcgcg
     4441 accttcccgg tgattatgtc actggtaggt ctggcggtga tcatcggcct ggaaaaactg
     4501 aaagtccctg gtggcattct gctgaccatt atcggtatct caattgtcgg tttgatcttc
     4561 gatcctaacg tccatttctc cggcgttttc gccatgcctt cattgagcga tgaaaacggc
     4621 aattcactga ttggcagcct ggacattatg ggcgcgctga atcctgtagt cctgccaagc
     4681 gttctggcgc tggtgatgac ggcagtattt gatgccaccg gaactatccg cgctgtcgcc
     4741 ggccaggcga acctgctgga taaagatggg cagatcatcg acggcgggaa agcactgacc
     4801 actgactcca tgagcagcgt tttctctggc ctggtgggtg cagctccggc agcggtatac
     4861 atcgagtctg cggcaggtac ggcggcgggc ggtaaaaccg ggttgacggc tatcaccgtt
     4921 ggcgtgctgt tcctgctgat tctgttcctc tctccgcttt cttacctcgt tccggggtat
     4981 gcaacggctc cggcgctgat gtacgttggc ctgctgatgc tgagcaacgt ggcgaaaatc
     5041 gactttgctg attttgttga tgcgatggcg ggtctggtta cggcggtatt catcgtgctg
     5101 acctgtaaca tcgtaacagg catcatgatc ggcttcgcga ctctggtgat tggtcgtctg
     5161 gtttccggtg aatggcgcaa gttgaacatc ggtacggtcg ttatcgccgt ggcgctggtg
     5221 accttctatg cgggtggctg ggctatctaa tctttcatcg gattctgaaa acgggtggca
     5281 atggctgccc gtttttattt tctccgcaca ttgtgtgagc ttttgcgata ttctgaaaaa
     5341 atgagaattc aggcataacg tctttctcag atcataaaaa acatcgcaaa cagggaacgc
     5401 atggaaatct tcttcaccat actgataatg accctcgtgg tctcgctgtc cggggtggtc
     5461 actcgtgtca tgccctttca gatcccgctt ccgcttatgc aaatcgccat cggtgcgcta
     5521 ctggcgtggc cgacgtttgg tttgcatgtg gagtttgatc ctgaactctt tttagtcttg
     5581 tttatcccgc cgttgctgtt cgctgatggc tggaaaacgc cgacccgtga atttctcgaa
     5641 catggtcgag agattttcgg cctcgcactg gcgctggtgg tggtcaccgt ggtcggcatt
     5701 ggcttcctta tttactgggt ggtgccgggc attccgctga tccccgcctt tgcgctggcg
     5761 gcggtacttt ctccgaccga tgctgtggcg ctctccggga ttgttggcga agggcgcatc
     5821 ccgaaaaaaa tcatgggcat tttgcagggc gaagcgttga tgaatgacgc ctccggtctg
     5881 gtgtcgttga agtttgccgt agcagtggcg atggggacga tgatcttcac cgtcggcggt
     5941 gcgacggtcg aatttatgaa agtagccatt ggcggtattc ttgccggttt tgttgtgagc
     6001 tggctgtatg gtcgctcgct gcgattcctc agccgctggg gcggtgatga acccgcgacg
     6061 cagattgttc tgctgttctt gctgccattc gcttcttatc tgattgccga acatattggc
     6121 gtttccggca tcctcgctgc ggttgccgcc gggatgacca tcacccgctc cggtgtgatg
     6181 cgccgtgcgc cactggcaat gcgcctgcgc gcaaacagca cctgggcgat gctggaattt
     6241 gtttttaacg gcatggtgtt cctgctgtta ggtctgcagc taccgggtat tctggagacg
     6301 tcgctgatgg cagcagaaat cgaccctaac gtcgaaatct ggatgctgtt taccgatatt
     6361 attctgatat atgcggcgct gatgctggtc cgtttcggct ggctgtggac gatgaaaaag
     6421 ttcagcaacc gcttcctgaa gaagaagccg atggagtttg gttcgtggac cacacgagaa
     6481 atcctgatcg cgtctttcgc cggggtgcgt ggggcgatca ctctggccgg tgtgctctct
     6541 atcccgctgc tcttgccgga tggtaacgtc ttcccggcgc gctatgagct ggtgtttctg
     6601 gcggctggtg tcattctctt ctcgctgttt gtcggcgtgg tgatgttgcc tattctgcta
     6661 caacacattg aagtcgcgga ccattcgcaa caattgaaag aggaacgtat tgcgcgagcg
     6721 gcaacggcag aagtggcgat tgtggcgatc cagaaaatgg aggagcgtct ggcggcggat
     6781 accgaagaga atatcgataa ccagctgctt acggaggtca gttctcgcgt cattggtaat
     6841 ctgcgtcgtc gcgccgatgg acgtaacgac gttgaaagtt ctgtgcagga agagaacctt
     6901 gagcgtcgct tccgtctggc ggcattgcgt tctgaacgtg ctgaactgta ccacctgcgc
     6961 gccacgcggg agatcagcaa cgaaacgctg caaaaattac tgcacgatct cgatttgctt
     7021 gaagcgttgt tgattgagga aaatcagtaa gccgaaagcg acgtaaccag gcccgaggta
     7081 atttgaaaaa ggtgcaatct tttgcacctt taacaggtta gctcgggcta acattcatga
     7141 ttctaaaaca aataagttat ctctcttgta aagtcaattc attggctgtt ctccgactta
     7201 acctcaatct gcgtgttgca taatatctgc agtaatcttc atagcgactg catgaatatt
     7261 atcctcagtt tgtgacatgc cagtatgaga ggtacaatct tcttcaaaga atttctgtag
     7321 tgttaccagg gtatcactat caatggggat attttcctcc ccacggagaa tggaaatgcc
     7381 cattccctgg acgctagcct catcgaaatt aacacgatag agacggatat taaacatcca
     7441 ggcttttgtt aaaacagaac agttgaaatg gcaattgctt aaattggcat tgttaaaatt
     7501 aacattagcc atattagagc cggtacatat aaccatttcg aggaacgtat ttgttaaccg
     7561 cgtaaaaaag agtttgctat caattaagtt tatattctgg agggttgctt tattcaggtt
     7621 gacacgagac agatcagcat gttcaaagtt aattaaatca agtttagaga agctaaggtc
     7681 ggctctaaat aaattaatat atggcgctat ta
//
LOCUS       DAZUCE010000229         7601 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZUCE010000229 DAZUCE010000000
VERSION     DAZUCE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.229"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..924
                     /gene="yjeM"
                     /locus_tag="ACHY03_004264"
     CDS             <1..924
                     /gene="yjeM"
                     /locus_tag="ACHY03_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFS3907432.1"
                     /translation="NFLASPNPGYQSGLAMLSFVVFAIFAYGGIEAVGGLVDKTENPE
                     KNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGSVNLGNITYVLMKSLGVTLG
                     NALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYSPLKAIIQGTPKALWPEPMT
                     RLNEMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNKLTLMANVSMTLPYLFLALA
                     FPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANVFTIIQPVVEAGDWDSTLWM
                     IGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(1075..4398)
                     /gene="mscM"
                     /locus_tag="ACHY03_004265"
     CDS             complement(1075..4398)
                     /gene="mscM"
                     /locus_tag="ACHY03_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFS3907433.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4420..5388)
                     /gene="asd"
                     /locus_tag="ACHY03_004266"
     CDS             complement(4420..5388)
                     /gene="asd"
                     /locus_tag="ACHY03_004266"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFS3907434.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLENLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5485..6537)
                     /gene="rsgA"
                     /locus_tag="ACHY03_004267"
     CDS             complement(5485..6537)
                     /gene="rsgA"
                     /locus_tag="ACHY03_004267"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFS3907435.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLDPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6632..7177
                     /gene="orn"
                     /locus_tag="ACHY03_004268"
     CDS             6632..7177
                     /gene="orn"
                     /locus_tag="ACHY03_004268"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFS3907436.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7388..7463
                     /locus_tag="ACHY03_004269"
     tRNA            7388..7463
                     /locus_tag="ACHY03_004269"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7421..7423,aa:Gly,seq:gcc)
BASE COUNT         1736 a         2015 c         2037 g         1813 t
ORIGIN      
        1 aatttccttg catcaccgaa cccgggttat cagtccggtc tggcaatgct atcgtttgtg
       61 gtatttgcta tttttgccta tggcggaatt gaagcggttg gtggtctggt cgataaaacg
      121 gaaaatccag aaaagaactt tgccaaaggt attgtttttg ccgctattgt tatttcaatc
      181 ggttattcgc tggcaatatt tttatggggc gtcagcacaa actggcagca ggtattaagt
      241 aatggttccg ttaaccttgg caatattacc tatgtgctga tgaagagcct cggggtgacg
      301 ctgggtaacg cactgcattt gtcacctgaa gcgtcattgt cgctgggagt atggtttgcg
      361 cgtattaccg gactttcgat gttcctcgct tataccggtg cgttctttac gctttgctat
      421 tcaccgctga aagccatcat ccaggggacg ccgaaagcgt tgtggccgga accgatgacg
      481 cgcctgaatg agatggggat gccgtctatc gccatgtgga tgcagtgcgg gttggttact
      541 gtcttcatcc tgctggtttc gtttggtggc ggtaccgcat cggcgttctt taacaagctg
      601 acgctgatgg cgaacgtgtc tatgacgctt ccttacctgt tcctcgcgct ggctttccca
      661 ttctttaaag cacgtcagga tctcgacaga ccatttgtga ttttcaaaac gcgtatgtcg
      721 gcaatgattg cgacggtggt tgtcgttctg gtggtgacat ttgcgaacgt cttcaccatt
      781 attcagcctg tggttgaagc cggagactgg gacagcacat tgtggatgat tggcggccct
      841 gtcttcttct cgctgttagc gatggcgatt taccagaact attgcagtcg catggcgaat
      901 aaacctgagt tagctctcga ctgatcatat gaaatcctgc aactcatcgt ctctccggcg
      961 atgagttgtc aggaagtaat gcgtatggat gatactctcc agggccggtg ataactgaag
     1021 atgatgttaa gctgctaaat gagccacgtt gacatcattt ggcagcttca tgcattacaa
     1081 acttcccgcc tgacgacctt tgcccgcaga cgtcagcgtt ctccccgttt gtttaccgtt
     1141 gaggctttcc agacgcatct ggaagggcgg gaatggcata tcgataccgt gggcatggaa
     1201 gccagccaga atcagctggt ggatctcatg gcgtagcggc atacggtgac ccatctcagc
     1261 ggcgtaaata cgcagctcga aaatctgaat cccctgttgc agatccacca ggaagacttc
     1321 cggtgccggg ttgtcgatca ccagcgagca gcgacgcgct gcggtgagca ggatttccgt
     1381 cacttcttca ctgttggcat cggcaggggc cggaatcgtc aataccacgc gcgtgaccga
     1441 gtcagagagc gaccagttga taaactgctc ggtaataaac gccttgttcg gcacgattat
     1501 ctctttacgg tcccagtcgc tgatggtggt ggcgcgggtg ttaatttttg tcacgctacc
     1561 agtaagatcg cggatcgtta ccgtatcgcc aatgcgaatc ggtttttcga acaggatgat
     1621 caggccagag ataaagttgg cgaaaatttc ctgcaaacca aaaccgagac caacaccgag
     1681 cgcggcaacc agccactgca atttcgacca ctcaatacca atcattgaga agccgaccag
     1741 cccgccaatc agcatcagca gatatttggt gatggtggtg atggcgtaac ccgtacccgg
     1801 cgttaaatcc aggtgctgca aaatcgccag ttccagcagc gcgggcaggt tgcgcaccag
     1861 ttgcgtggtg atgataaaca ccagaatggc aatcagcacc gcaccgaggg taattggctc
     1921 cagactttct acgccctgta ccgtggaggt gacatcccac agcgaaatat tttcgaggaa
     1981 gccgaaagcg gaatggattt ctgaccacag cacaatgacc gaaagcaggg cgatcaacat
     2041 caaaattgag cgcaccagcc gcaaggattg cgcactaatg gcatcgagat cgacttcgct
     2101 ttcatcgact tcaattgccc cttccgggct actgtgatga tgcgcttcct cttcgccacg
     2161 cgcgcgttgc gctaacatct ctgcccggcg atgcttcgcc cggtcaaacg ccagcctgcg
     2221 gcgctggatc agcatccagc ggcggataac gtgataaacc accagtagca ggaaccagat
     2281 ggcaaccgag gtttcaagcc tcgctaacag tgcctgcgcc gttgccagat aacccaccgc
     2341 cgacgccaga atggcaacca gtggcgcgcc aatcatcatg ttccacagca tatggttggt
     2401 aatgttgtcg ccgctgccct ctttgttgag atacagcggt atcccggcct ttttcaggct
     2461 gagggtgacc accgccagcg caccgcaaat gaggataaag caaagccgtc ccagcgaacc
     2521 ggaaaattcg cggtcgtcga ggttatcgaa catcatcagc gccataatca gtggcacaat
     2581 aagcccgatg ctcatcaggt agtagcgcat cccacgggaa acacgttcgc gcggccaacc
     2641 aaaatgagcg ataaacaagc cgttcgggcg ggcaaaggta gcgcaaatca tcactaccca
     2701 cagcagcggc acggtggctg tgacgccatc accaatcgcg accgccagcg gataaggcca
     2761 cgcctcgcgc aagccgtaac ccagcgtcat ccacagcacc ggcagcggtg acgcgacgag
     2821 aatcgaccag aaaagagtgc gtaacgtcag ccagaagtga tcctgagtca ctttaccgac
     2881 tttcgccgcc gaacgttcaa gaaaacgggt gaaatagcgg cgcgagtaaa tactgcaacc
     2941 gaccagaatc aacgcgccaa acagcggcaa aatcgtctct ttgctggtca gcatcatcac
     3001 actggctttg cccaactgac tgaaggtgtc cagcgaaatg agacgacgca gatcctgggc
     3061 gatttccagc ggccaggcga tggtcatcgg gcgcacgtca gaggtccaga acagatagcg
     3121 gtgcgtcgct tcgttcacct ctttcagcgc gtcctccagt tgcccgttag agactttcag
     3181 cttggtcagt tccagcagta gtgtgtcgcc accctgcaac aatgagttca gcaactcacg
     3241 ctgagtgcgt agctgtgctt cgagaatacg gttttgctcg gcagtcagcg gctgaccgtc
     3301 ggcctgatga atttgccgta gcagcggctg tttattaagc agatcctcat aacgtaaccg
     3361 ttgcacacgc aactgcgcca tttcggtatc aagctgttgt ggtttcggca tttccggcag
     3421 ccgtgccacc tgcgcccgca acgcttcacc gagcagattg gacgatccca gccattgcga
     3481 ctgttcacgc agcgtattca acgcctgccg gacctgtaac gtctggctgg cagcctgacg
     3541 ctgttgcgag gcaacgagat ccatccgctg cgcctgttga ttcaaagccg ccgatagttc
     3601 gcggttaatt ttgaattgcg cgacgatatc tttcggcaaa tcggcactgt tttctgctag
     3661 taattcggta ctttccagcg cccgctctgc ctcaagctgg cgttggctgt tcaattgatt
     3721 acgcaaggcc tgcaaatacg catccagttg ctggctctct ttttccgcca gctctgagcg
     3781 caagcgcgct aattcctggc ggttattggc agacagctgc gccagctcca gttcatcaac
     3841 gagcgcctta agacgtgcgg agtcagactg caacgcgaaa ttttgtgcct gattgagcgg
     3901 agtattgccg gtaagcgttc ccaggcggcg ctcgatctcg tttaactgac ggcgggcgtc
     3961 ggtttgctgt tgcggcagtt gattcagcga atcggcaatc tcgcgggcgc gctcctgctc
     4021 ttgctgggcc tgacggcttt tatccagcaa ctgactgctg acctggagaa tttcctgatt
     4081 cagcgcgtcg gtagacatcc ccggcgacac gctgcgcggc tcgtcacgca tgttgtttaa
     4141 ctgcgcgcga agagtcgcgg agagtttcgg ataattatca ataacttctt gatattgctt
     4201 gatgcgctca agggaacctt ttcgttcctc aagcgcattt aaggcagact ggagcgcctc
     4261 tacgacttcc ggctgcgcgg gtttcgccgc ttttgcctgc tccagttcct gggtgatttg
     4321 tttgctatcg ggggccgtcg cggcgtacgc cccccaactg aggcaccagg ccatcagaaa
     4381 agtgataatc aggcgcacgt cagtgtttcc tttgatggat tagacctggt cttttttatc
     4441 gtcaaccaat gggctggcgt cgtgttctgc ttcgatctct tcagcaggaa gcggggcggg
     4501 ttcagcgtct ggcgtaacaa aggtttcggt agatactgcc agcggctggc caattttcgt
     4561 gacagacagg ttttccagtt gctcaaccag attcacttta cccggtgcaa acaggttgat
     4621 aacggtggaa ccgagtttaa agcgacccat ttcctggcct ttcagcagtg ccacagaacc
     4681 gtcgttttcc ccggcaggcc aggtccagcg cttgatgatg ccttcgcgcg gcggcgtaat
     4741 ggtgcccgcc cagacagtct caatgctgcc tacaatcgtc gccccgacca gaatctgcgc
     4801 cattgggcca aattcggtat cgaacaggca gatcacgcgt tcgttacggg caaacagatt
     4861 cggcacgttc tgagccgtga gatggttaac ggagaagaga tcgcccggca cgtagatcat
     4921 ctcacgaaga ataccgttgc acggcatgtg tacgcggtgg tagtcacgcg gggagaggta
     4981 agtggtcaca aacgtaccgt tgcggaacag gtccgccatc aggtagttac ctgccagcag
     5041 ggcttcgagg ctatagttgt ggcctttggc ttgcaggatt ttatcttctt cgattttacc
     5101 caactggctg ataacgccat cggcaggcat aaccaggaca ttcggatcgg tatcgattgg
     5161 gcgtacttcg tcacgcagcg gacggacaaa gaattcgtta aaggtgcggt agctggcggt
     5221 gtccggcttt tgcgcctctt tcatgtcgac cttgtagtat ttaacgaaca gatcgataac
     5281 cagttttgtc agccatcctg cccgcttgct tgcgccccaa cccgccaggc gagtaagcca
     5341 tagtttcggc agaatgtact gtagcgaaag tttaaatgaa tttaacaaag tagcctccag
     5401 gccattgttt tgtcgttcct gatccggcct acatgccgga tcctgaaaaa aaggggacga
     5461 ttctaacgac ggttagctta attgtcagtc atccgtatca gaaaagtttt tacgcgtttt
     5521 tacctgcgcc atgctttcca gaatacggtg atagttttcg aaacgggttt ccgcgatttt
     5581 tccttcctca accgcttccc ggatagcgca gcccggatcg gtatcgtgtt tgcaatcgcg
     5641 atatttacac agacctaagt agtcatggaa ttcgacaaaa ccctgagtga tttgttccgg
     5701 atccaggtgc cagaggccga actcacgcac accaggggaa tcaatcacat caccgccgtg
     5761 cgggaagtga tacagccgag cggcggtcgt ggtgtgctga ccgagccccg agttgtcaga
     5821 gacatcgttg gtcaggatct ctttttgcag ccccagcaac gcgttcagca ggctggattt
     5881 accaacgcca gactgcccgg caaaaatgct gatgcgcccg gtcaacgcct cttccagcgg
     5941 ttttagccca tcctgagtat ggctggaaac catcaatacg cgataaccga tattgcggta
     6001 gatatccatc tgctcgttga cgaactccat accttcgtcg tccagcaggt ctatcttgtt
     6061 gagcacaata atcggctcaa tctgcaaggt ttcgcaggcc accagataac ggtcgataat
     6121 attgagcgac agctccggca aaatggcgga gacaatgaca atctggtcga tgttggcggc
     6181 aataggtttc acgccgtcgt agaaatccgg acgcgtcaac actgaggtgc gctcatgcac
     6241 cgcttccacg atccctttga catttacgcc ttccgccgcc ggtttacccg gacgccagac
     6301 tacgcggtcg ccggtcacca gcgaacggat ggtacggcga atattgcagc ggtgaacgtc
     6361 gccatcggcg gattccacat cagcgtgcat accaaagcgg ctgatgacga taccttcatc
     6421 aggctcgcca aacagattgt cgtcgtagtc gggcttctcc ttagacgttt taagacgacg
     6481 ctggtgattg gcgttcacgc ggcgctgctg gcctttggag agtttatttt tactcaatcg
     6541 tacagactcc tggtcgcccc tgatgggcaa aacatctatg atacacgcaa ttgtggatca
     6601 atatagtcac tgtgaatggg tggaaaatag catgagtgcc aatgaaaaca acctgatttg
     6661 gatcgatctt gagatgaccg gtctggatcc cgagcgcgat cgcattattg agattgccac
     6721 gctggtgacc gatgccaacc tgaatattct ggcagaaggg ccgaccattg cagtacatca
     6781 gtccgatgaa cagctggcgc tgatggatga ctggaacgtg cgcacccata ccgccagcgg
     6841 gctggtggag cgcgtgaaag cgagcacgat gggcgatcgt gaagctgaac tggcaacgct
     6901 cgaattttta aaacagtggg tgcctgcggg taaatcgccg atttgcggta acagcatcgg
     6961 tcaggaccgt cgcttcctgt ttaaatacat gccggagctg gaagcctact tccactaccg
     7021 ttatctcgat gtcagcaccc tgaaagagct ggcgcgccgc tggaagccgg aaattctgga
     7081 tggttttacc aagcagggga cgcatcaggc gatggatgat atccgtgaat cggtggcgga
     7141 gctggcttac taccgcgagc attttatcaa gctgtaaaat ttttcctggt cacgtaagcg
     7201 tcggaatcca cttgcggcgg cgtgattttg acgctaaatt gaacgccttg ctgataattt
     7261 cagcacttga gataaaaact caaaaaaaat tttttagggg ggttgcagag ggaaagattt
     7321 ctcgtataat gcgcctcccg taacgacgca gaaatgcgaa aattacgaaa gcaaaattaa
     7381 gtagtacgcg ggaatagctc agttggtaga gcacgacctt gccaaggtcg gggtcgcgag
     7441 ttcgagtctc gtttcccgct ccaaattctt ctctcaataa aatatccaca gcgacgcgat
     7501 gcgttattgc tggtttttgt tgtctctgac aaactcttgt aaacagagtt atccacagcc
     7561 tcaggccgta atcttaattt caaagaaact tcgcacggtg a
//
LOCUS       DAZUCE010000230         7598 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZUCE010000230 DAZUCE010000000
VERSION     DAZUCE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.230"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(322..1281)
                     /gene="nrdF"
                     /locus_tag="ACHY03_004270"
     CDS             complement(322..1281)
                     /gene="nrdF"
                     /locus_tag="ACHY03_004270"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS3907437.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRSDEPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1291..3435)
                     /gene="nrdE"
                     /locus_tag="ACHY03_004271"
     CDS             complement(1291..3435)
                     /gene="nrdE"
                     /locus_tag="ACHY03_004271"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS3907438.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFDHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYNENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGQTFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3408..3818)
                     /gene="nrdI"
                     /locus_tag="ACHY03_004272"
     CDS             complement(3408..3818)
                     /gene="nrdI"
                     /locus_tag="ACHY03_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFS3907439.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3815..4060)
                     /gene="nrdH"
                     /locus_tag="ACHY03_004273"
     CDS             complement(3815..4060)
                     /gene="nrdH"
                     /locus_tag="ACHY03_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFS3907440.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4308..4637)
                     /locus_tag="ACHY03_004274"
     CDS             complement(4308..4637)
                     /locus_tag="ACHY03_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFS3907441.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4789..5133
                     /locus_tag="ACHY03_004275"
     CDS             4789..5133
                     /locus_tag="ACHY03_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFS3907442.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5170..5619)
                     /gene="alaE"
                     /locus_tag="ACHY03_004276"
     CDS             complement(5170..5619)
                     /gene="alaE"
                     /locus_tag="ACHY03_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFS3907443.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            6287..6691
                     /gene="stpA"
                     /locus_tag="ACHY03_004277"
     CDS             6287..6691
                     /gene="stpA"
                     /locus_tag="ACHY03_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFS3907444.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(6744..7262)
                     /gene="ygaP"
                     /locus_tag="ACHY03_004278"
     CDS             complement(6744..7262)
                     /gene="ygaP"
                     /locus_tag="ACHY03_004278"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFS3907445.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQ"
     gene            complement(7272..7571)
                     /locus_tag="ACHY03_004279"
     CDS             complement(7272..7571)
                     /locus_tag="ACHY03_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFS3907446.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT         1980 a         1815 c         1844 g         1959 t
ORIGIN      
        1 atacattacc caatggaaat agtcactttt ttctacccta acatactgag aatctgaggc
       61 aacccctgag ggcaaatgtg gcatgaatat tgatgtgagc aaatcgccgg aatttcccat
      121 gatataaggg ctgagagcaa atcgagaaaa gttgatattt tttgtcaaag atagcaaaaa
      181 aacgcctgaa tattgtggtt tcaggcgaat aatgcaacag agtatcagtg tagatcacca
      241 caaattattt acgtgatata aagctatttg gcgggaatta tttccctgca gcgggtagtg
      301 atatttttga aaataacccc ctcagaaatt ccagtcttca tcttctgttt cgaccgcttt
      361 tcccatcaca tatgaagagc ctgaaccgga aaagaaatcg tgattttcat cggcattcgg
      421 cgaaagcgcg gcgaggattg ccggattcac ttccgccatt tctgcgggaa atagcggttc
      481 gtagcccaga ttcatcaacg ccttattggc gttgtaacag agaaacgctt tcacatcgtc
      541 agcccacggg gtttcggcgt acagctcatc ggtgtattgc aactcgttgt catagagttc
      601 cagcaacaaa tcgaaggcga aactcttcaa ttcttcacgt cgtgcctggg agatcttttc
      661 caggtttttc tgatatttat agccgatgta gtaaccgtgg actgcttcat cccggataat
      721 cagacggatc aggtctgcgg tattggttag ctttccgcgg ctggaaaaat acatcggcag
      781 ccagaaaccg gaatagaaca aaaaagattc aagaaacaca ctggcgattt tctttttcag
      841 cggttcatca ctgcgataat gttgctgaat aatctgtgct tttcgctgca acggcgcgtt
      901 ttcttcactc caggcgtagg cggcatcgac atctttggtc tggcacagcg tcgagaaaat
      961 tgaactgtaa gagcgagcat gaaccgcttc cataaagctg atattcgata atactgcctc
     1021 ttcatgaggc gtgagcgcat cgggcatcag cgaaggcgcg ccgataacat tttgcagcgt
     1081 gtcgagcagc gtcaggccag taaaaacgcg cattgtcagt tgttgttcta cgacacttaa
     1141 tgtctgccat gcaggtatat cgttcgacag tggcaccttt tctggtagcc agaaattgct
     1201 ggtcaggcga ttccacacct ccagatcttt atcgtcagat atcttgttcc agttgatggc
     1261 gctgatacgt gagagtttca tagatattcc ttaaagtgca caggagacgc agccttcaat
     1321 ttcagtgcct tccagcgcca tctgacgcag gcggatgtaa tagagcgttt tgataccttt
     1381 gcgccaggcg taaatctgcg ctttgttgat atcgcgagtg gtggcggtat cggggaaaaa
     1441 cagcgtcagc gacagcccct gatcgacatg gcgagtcgct tccgcgtagg tgtcgatgat
     1501 cttttctgcg ccgatttcgt aagcgtcctg atacagcgcc agattttcgt tagtcataaa
     1561 tggggcaggg tagtaaacgc gtcctgtttt gccctctttg cgtatctcta ctttcgccac
     1621 aatcggatga atactcgacg tggcatggtt gatataagag atagaaccgg ttggcggcac
     1681 cgcctggaga ttctggttgt aaatgccgta gcgcatcacg tcatcgcgca gttgcgccca
     1741 catctcacgg gtaggtaacg taataccgct gcgggcaaac agttcgccaa ctttcgccgt
     1801 tttcggctgc cagttacctt gcagatattg gctgaaatat tcaccactgg catagcgtga
     1861 ctgtttgaac ccggcgaagg tttgaccgcg ttcgcgcgcc agcaacatcg aggtacgcag
     1921 tgcgtgccag gtgatggtat agaaatagag attggtgaaa tccagtgctt ccggcgaacc
     1981 ataagcgatg ccttctcgcg ccagataacc gtgtaaattc atctgcccca gtccgatggc
     2041 gtgcgaggcg gcatttccgg cttcgatgga cggtacgctg cgaatatgac tcatatctga
     2101 tactgccgtt aaaccgcgca cggcagtctc taccgtgcgg gcaaagtcgg gggaatccat
     2161 ggtgtgggca atattcaacg aacctaaatt gcaggagata tcatggcctg tgcgggcata
     2221 gtcgagattc tcgttatact ctgaggcgct gttaacctgc aaaatctctg agcagagatt
     2281 actcatattt atgcgcccgg cgatagggtt agcacggttt accgtgtctt catacatgat
     2341 gtacggatag ccggactcaa actggatttc tgccagtcgc tggaagaaat cacgggcgtt
     2401 gaggtatttt tttcgaatgc gttcatcggc aaccagttcg tcatagtgtt gactgatggc
     2461 cacatcggca aacggcttgc cataaactcg ctctacgtca taaggcgaaa acagcgccat
     2521 ctgcgcattc tcttttgcca gatggaaagt gatatccggg atcaccacgc caagcgacag
     2581 tgttttaatg cggatttttt cgtcggcatt ttcccgtttc gtgtcgagaa aacgcagaat
     2641 atcgggatga tgagcatgta aatagactgc accagccccc tgacgagcgc cgagttggtt
     2701 ggcataggaa aatgcgtctt ccagcatttt catcacagga attacgccag aagattgatt
     2761 ttcaatacgt ttaattggcg cgcccgcttc ccgcagattc gacagcaaaa atgctacgcc
     2821 gccgccgcgt ttcgacagtt gcagtgcgga atttaccgcc cgaccaatcg actccatatt
     2881 gtcttcaata cgcagcaaaa aacaggaaac cagttcgccg cgctgctgct taccgcagtt
     2941 gaggaatgtt ggcgtggctg gctgaaagcg tcctgacagc atctcatctg tcagttgcaa
     3001 cgccagcgtc tcatcgcctt gtgccagcgt cagcgccacc atcgtgactc gatcggcaaa
     3061 atcttccaga taacgtttac cgtcgaatgt cttcaacgta tagctggtgt aaaacttcca
     3121 tgccccgagg aatgtctgga aacgaaaacc gctggtgtgt gcgtggtcaa acagcgtaat
     3181 gacaaagtcg cgagagtagc gattaagaac gctttcatca tagtaacctt cgttgaccag
     3241 ccagttcagg cgctgctgct ggctactgaa ggtcacactg ttcggacgca catgcgtcgc
     3301 aataaaggcg tcaacggcct ggcggtcttt atcgaactga atgcgacctg cgctatcgta
     3361 gaggttaagc atcgcattca gcgcgtggta atccatcgtt tcctgcgtca ggcattctgc
     3421 ggttgtcgtt gccaaaattc ggttactcct ttacgaacgt tttcgatatc gctttgcgta
     3481 cccatgagtt caaaacggta tagccacggc acgccgcatt tccgggcaat cacatctccg
     3541 gcgcggccat acgcctcacc aaagttgcga ttacccgaag caataacgcc gcgaagcaac
     3601 gcccggttgt gctcgtcgtt taaaaagcga attacctgtc gtggcactgc gccagccgta
     3661 ccgccgccgc cgtaagaggg cacgatcagg atgtaaggct cgtctacctg aatccgttcc
     3721 cgctcattga gcgggatgcg caccgcgggc agacctaaac gttcgataaa acgctgcgtg
     3781 ttttcggagc tgctggagaa gtagacgagc tggctcatgc actggcagcg tgtggcgctg
     3841 gatgcagacg gttaatcatg tccggacgga aaccagacca gctaagatcg ccagcaatca
     3901 ctaccggcaa ctgacgaaag ccctgagcac gcaacgcttc tgccgcttca ggaacgcgat
     3961 cgacattaat catttcaaaa tcaaagcccc ggttttccat cgcccgtttg gtggcgtggc
     4021 actgaacgca atcgttacga gtgtaaatag taatgcgcat gattcgtatt tccgtttaaa
     4081 atgaagatac ggcgcgatga tacgcgtcgg gttgtctctc tgttgataca gagatactag
     4141 atgtagttga aaaaagattc aaccacacaa tatatagcaa atagtggtcg aaattaccct
     4201 ggatatgagc gtgacggggt agggggattt ttgtgattca ccagacaaaa agaaaccccg
     4261 aagccaggct tcggggtcaa agacgcgtat ttattatcat tgttgcacta cgatttacgc
     4321 atgctcaaca acgcaccgat aaaaatacct accgcagcag cagttcccac actacaccag
     4381 ggtctttcac gaacaaaaga atccgcgcag ccaacggcat cacgtgcggc ttgctggacg
     4441 cgagtacgac catgcattcg cgccctggtt tctttcagca atgcctgagc tttactgcgt
     4501 gcggcttcag cttccccttt ggcgtcgctg ccccaggact tcaatacaga ttcgaggctg
     4561 tcagctaatt gattgacatc attctggata tcctgcacgc catcatcaac gtcgttgcgg
     4621 ttcggtcggt taaacatatg atcccccatt agaattcgat gtaattttca gtgtagacca
     4681 taattttctt aactgaggtt catcgcggct ttatggactt ttctgaaagc gttgtgaatg
     4741 cgcaatcagg taaggtaggc ccgcggttga taataacgag gatcaaatat gtatttacga
     4801 ccagacgagg tggcgcgcgt acttgaaaaa gtcggtttta ctgtcgatgt ggtaacccag
     4861 aaagcgtatg gttaccgccg tggggaaaat tatgtctatg ttaatcgcga agcgcgaatg
     4921 ggacgtaccg cactggtaat tcatccaaca ttaaaagaac gtagttcgac actggctgaa
     4981 cccgcttccg atattaaaac ttgcgatcat tatcaacaat ttccgctcta tttagcaggc
     5041 gagcgacacg agcattacgg tatcccgcat ggctttagtt cgcgtgttgc gcttgaacgt
     5101 tatttgaatg gtttatttgg cgaagccagt taaagacgcg actggcgatg ccagtcgcga
     5161 aaagaagagt caggctttta cctgctggta acggctgact ttaaacagtc ggcggcaata
     5221 atcgaggaag tagccataaa ccgcccccat caacatcgaa acaacgatgt ttgaactgac
     5281 cgccgccata atctgatgcc agtctgcgcc caccactaac aagatcgcca catacaccgg
     5341 tgactggaac gtcacataag ccaggatatc cgccagattt tttatccagc ccgacgggct
     5401 aactttgcgt gccgcgcgca taaacagatc gcggtacata ccgtatggcc atgcaattaa
     5461 gatgttgacc ggaatcgcta ccaatctgga ataaaaagac tgttcgaagc tcattccgga
     5521 gaggaaaact tcaatacaca tgttcacgac agaacagtaa acaaccatcg cgaacgtatc
     5581 tgcaactgca tgacgcaagc gtgactgcgg tgagaacatg ctaatgctcc ttaataaaag
     5641 cgtaatggga tgttaatgga gatgaaagca gtggatctca ttgccatctg cattttcgca
     5701 tagcgtatat cgctggctat gactaatcaa ttagtgaaaa atttttattt agtcggtttt
     5761 tgtagataaa atattccgta acggctcgtt ttttgcacat cgtggatttt tacatgccga
     5821 tatgatttat tttcaattaa atcaattaga tatggccttg cctttaattt cactaatgaa
     5881 agtttggggg tgaggtaacg ctataagcgt agagaattcg gtgtatgtcg tgaccgggtc
     5941 atttacagca ttttttatgc cagaaaatgt acattaacag cagaaagcac cagtgagggg
     6001 tgttttcttt tattctatta tattgcattt ttattcactt cggttttgtc aggcttgcgg
     6061 aattagcgag cagagagcgc cactctgttc accaataacc taaattactc atcaaaatgg
     6121 ctaatatata atcttgaaat tattctgaga gtttcagaaa ataacagctg aaataatctc
     6181 gcgcaggact gtaaatagat taaattttgt ggaaatataa taagtgatcg cttacactac
     6241 gcgacgaaat acttttttgt tttggcgtta aaaggttttc tttattatgt ccgtaatgtt
     6301 acaaagttta aataacattc gcaccctccg tgcgatggct cgcgaattct ccattgacgt
     6361 tcttgaagaa atgctcgaaa aattcagggt tgtcactaaa gaaagacgtg aagaagaaga
     6421 acagcagcag cgtgaactgg cagagcgcca ggaaaaaatt agcacctggc tggagctgat
     6481 gaaagctgac ggaattaacc cggaagagtt attgggtaat agctctgctg ctgcaccacg
     6541 tgctggtaaa aaacgccagc cgcgtccggc gaaatataaa ttcaccgatg ttaacggtga
     6601 aactaaaacc tggaccggtc agggccgtac accgaagcca atcgctcagg cgctggcaga
     6661 aggtaaatct ctcgacgatt tcctgatcta attactgagg ccggatgtcg ctgctatggc
     6721 gcgtccggcg tcacttctca agctcattgg ttccacggca tcttatctaa caaccttgcc
     6781 atcccacaaa aaccgctaat tcctgcaaac agtaacccgg caccaacaaa gccgcttaat
     6841 aagaagaaac cgctatttac ggtataaccc agtataacgc cgattaatat taaaccaccc
     6901 gcagcgatct gcacctggcg cattaacggc aagggttgag atttatttac cgctactggc
     6961 aatcctgctt ttttccagcc atcaatccca tcttcgagta aaaagatttc tgcgggggcg
     7021 gcaatcgctg ctaatttatc ggcgttatta ctggtgcgtt tacctgcctg gcagtggaat
     7081 ataatttgct cgcgacgtaa tttagccggg agacctgact gttccagcac ggataatgga
     7141 gccagatctg cttcaggaat atgttcacga agatattcat cagcatcacg aatatcgatt
     7201 aactttgcgc cgcgtgcgat taattcttgt gcatcatgcg gcgaaatggt tgtcaaagcc
     7261 atagtgactc cttacggaca atagacattt ttcagggtgg cgataatggc atttaccgcc
     7321 tcatttttaa tggaatatag aatgcgttgg gcatcccgtt ggctgtcgat aagcccttcg
     7381 tcccgcatac gagcgagatg ctgtgatgtc gcagaggcgc tcagtccggt aatgcgcgtc
     7441 agctctcccg cgctggtgcc gggggaaccg ctaagcatgc acagaatcag caaccgttta
     7501 gggtggctca ttgctttcaa taaggccgct gcctgttcgg cactggcctg taattgcgcg
     7561 agttcagtca tatttattta agtattttct aaattaag
//
LOCUS       DAZUCE010000231         7557 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZUCE010000231 DAZUCE010000000
VERSION     DAZUCE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.231"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            103..1500
                     /gene="ulaA"
                     /locus_tag="ACHY03_004280"
     CDS             103..1500
                     /gene="ulaA"
                     /locus_tag="ACHY03_004280"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS3907447.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1516..1821
                     /gene="ulaB"
                     /locus_tag="ACHY03_004281"
     CDS             1516..1821
                     /gene="ulaB"
                     /locus_tag="ACHY03_004281"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFS3907448.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1831..2295
                     /gene="ulaC"
                     /locus_tag="ACHY03_004282"
     CDS             1831..2295
                     /gene="ulaC"
                     /locus_tag="ACHY03_004282"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFS3907449.1"
                     /translation="MKLRDSLAVNKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2309..2959
                     /gene="ulaD"
                     /locus_tag="ACHY03_004283"
     CDS             2309..2959
                     /gene="ulaD"
                     /locus_tag="ACHY03_004283"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFS3907450.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2969..3823
                     /gene="ulaE"
                     /locus_tag="ACHY03_004284"
     CDS             2969..3823
                     /gene="ulaE"
                     /locus_tag="ACHY03_004284"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFS3907451.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNVIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGMGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3823..4509
                     /gene="ulaF"
                     /locus_tag="ACHY03_004285"
     CDS             3823..4509
                     /gene="ulaF"
                     /locus_tag="ACHY03_004285"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFS3907452.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4638..4913)
                     /gene="yjfY"
                     /locus_tag="ACHY03_004286"
     CDS             complement(4638..4913)
                     /gene="yjfY"
                     /locus_tag="ACHY03_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFS3907453.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5240..5635
                     /gene="rpsF"
                     /locus_tag="ACHY03_004287"
     CDS             5240..5635
                     /gene="rpsF"
                     /locus_tag="ACHY03_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFS3907454.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5642..5956
                     /gene="priB"
                     /locus_tag="ACHY03_004288"
     CDS             5642..5956
                     /gene="priB"
                     /locus_tag="ACHY03_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFS3907455.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5961..6188
                     /gene="rpsR"
                     /locus_tag="ACHY03_004289"
     CDS             5961..6188
                     /gene="rpsR"
                     /locus_tag="ACHY03_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFS3907456.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6230..6679
                     /gene="rplI"
                     /locus_tag="ACHY03_004290"
     CDS             6230..6679
                     /gene="rplI"
                     /locus_tag="ACHY03_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFS3907457.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(6750..7544)
                     /gene="yjfZ"
                     /locus_tag="ACHY03_004291"
     CDS             complement(6750..7544)
                     /gene="yjfZ"
                     /locus_tag="ACHY03_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFS3907458.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATRCNAYDGITMNGDNSFGWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         1800 a         1837 c         2065 g         1855 t
ORIGIN      
        1 aatcataaat aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg
       61 ggtgtgtgga atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac
      121 atctttaccg tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg
      181 acctgtctgg gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt
      241 aaaaccataa ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc
      301 aaaccggtgg tggcgaaaat gtccgaagtc tacggcatta acggcgcaat ttcagatact
      361 tacgcttcga tgatggcaac catcgaccgc atgggcgatg cctatagctg ggtaggttac
      421 gcggtgttgt tagcgctggc actgaacatc tgttacgtgc tgctgcgtcg cattaccggt
      481 attcgtacca tcatgctgac cggccacatt atgtttcagc aggcggggct gattgccgtc
      541 acgctgttca tctttggcta ctccatgtgg accaccatta tctgcaccgc gattctggtt
      601 tcgctctatt ggggcattac ctccaacatg atgtacaagc cgactcagga agtcaccgac
      661 ggctgtggtt tctccatcgg tcaccagcaa cagtttgcat catggattgc ctataaagtt
      721 gcgccgttcc tcggcaaaaa agaggaaagc gttgaagacc tcaaactgcc aggctggctg
      781 aatattttcc acgacaacat cgtctccacg gcgattgtca tgaccatctt ctttggtgcc
      841 attctgctct ccttcggtat cgacaccgtg caggcgatgg cgggcaaagt gcactggacg
      901 gtctacatcc tgcaaaccgg tttctccttc gcggtggcga tcttcatcat cactcagggc
      961 gtgcgcatgt ttgtggcgga actctctgaa gcatttaacg gtatctccca gcgcctgatc
     1021 ccaggtgcgg ttctggcgat tgactgtgcg gctatctata gcttcgcgcc gaacgccgtg
     1081 gtctggggct ttatgtgggg caccatcggt cagctgattg cagttggcat cctggtcgcc
     1141 tgcggctctt ccattctgat tattccaggc tttatcccga tgttcttctc caacgccacc
     1201 atcggcgtgt tcgctaacca cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg
     1261 atggggatga tcgaaatctt tggttgcgtc tgggcggtga aacttaccgg tatgagtgcc
     1321 tggatgggta tggcggactg gtcgattctg gcaccgccga tgatgcaggg cttcttctcc
     1381 atcggtatcg cctttatggc cgtcatcatt gtaattgcac tggcttatat gttcttcgct
     1441 ggccgcgcgc tgcgcgcaga agaagatgca gaaaaacaac tggcagaaca gtctgcttaa
     1501 taaggagttt tgattatgac cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt
     1561 tccatgatca tgaagatgaa agtggaccag tttttaaccc aatcaaacat tgaccacacg
     1621 gtaaacagct gcgcggttgg cgaatacaaa agcgagttga gcggcgcgga tatcatcatc
     1681 gcttctacgc acattgcggg cgaaatcacc gtgaccggca acaaatacgt ggttggcgtg
     1741 cgcaacatgc tctctcctgc tgactttggc ccgaaactgc tggaagtgat caaagagcat
     1801 ttcccgcagg atgtgaagta aggacgcgcc atgaaattac gtgattcgct ggctgtaaat
     1861 aaatccatcc gcctgcaggc tgaagctgaa acctggcagg acgcggtgaa aatcggcgtc
     1921 gatctgctgg tggcggcgga cgttgtcgag ccgcgctatt accaggcgat tctggatggc
     1981 gtggaacagt ttggccctta tttcgttatc gctccggggc tggcgatgcc gcacgggcgg
     2041 ccggaagagg gtgttaagaa aaccggtttc tctctggtaa cgctgaaaaa gccgctggaa
     2101 tttaaccacg acgacaacga tccggtggat atcctcatca ccatggcggc ggtcgatgcc
     2161 aatactcacc aggaagtggg catcatgcag atcgtcaacc tgtttgaaga tgaagagaat
     2221 tttgaccgtt tacgcgcctg ccgtaccgag caggaagtac tggatttaat tgaccgcacc
     2281 aacgcggcag cttaagaagg aattgaacat gtcattaccg atgttgcaag tcgcgctgga
     2341 caaccagact atggatagcg cctacgaaac cactcgcctg attgccgaag aagtcgacat
     2401 tatcgaagtg ggcaccattc tgtgcgtggg cgaaggcgtg cgtgcggttc gtgacctgaa
     2461 agcgctctac ccgcacaaaa tcgtactggc agacgccaaa attgccgatg caggcaaaat
     2521 cctttcgcgt atgtgcttcg aagccaacgc tgactgggtg acggtaattt gctgtgcgga
     2581 tatcaacacc gccaaaggcg cgctggacgt ggcaaaagag tttaacggcg acgtgcagat
     2641 cgaactgacc ggttactgga cctgggaaca ggcgcaacag tggcgcgatg caggcattca
     2701 gcaggtggtt tatcaccgca gccgtgacgc acaggccgca ggtgtggcgt ggggtgaagc
     2761 ggacattacc gcgatcaaac gtctttccga tatgggcttc aaagtcaccg tcaccggagg
     2821 cctggcgctg gaagatctgc cgctgttcaa gggtattccg attcacgtct ttatcgcggg
     2881 ccgtagtatc cgtgatgccg cttctccggt ggaagccgca cgtcagttca aacgttccat
     2941 cgctgaactg tggggctaag gagcggatat gttgtccaaa caaatcccgc ttggcatcta
     3001 tgaaaaagcg ctccccgccg gggagtgctg gctggaacgc ctgcaactgg caaaaacgtt
     3061 gggcttcgat tttgtcgaga tgtcggtaga tgaaacggac gagcgcctgt cgcgcctcga
     3121 ctggagccgc gagcagcgtc tggcgctggt caatgtgatt gttgaaaccg gcgtgcgcgt
     3181 gccgtccatg tgcctttctg ctcatcgtcg tttcccactg ggcagtgaag atgacgcggt
     3241 gcgggcgcag gggctggaga ttatgcgtaa agctatccag ttcgcccagg acgttggcat
     3301 tcgcgtgatc cagctggcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg
     3361 tcgtcgtttc cgtgacggac tgaaagagag cgttgagatg gcaagccgcg cgcaggtgac
     3421 gctggcgatg gagatcatgg attatcctct gatgaactcc atcagcaagg cgctgggcta
     3481 cgcgcactat ctcaacaatc cgtggttcca gctctacccg gatatcggca acctgtcggc
     3541 gtgggacaac gacgtgcaga tggagttgca ggccggaatg gggcatatcg tcgcggtgca
     3601 tgtgaaagac accaaaccgg gtgtctttaa aaacgtgccg tttggcgaag gtgtagtgga
     3661 ttttgaacgt tgctttgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga
     3721 gatgtggagc gaaacggcgg aagacccggc ggcagaagtg gcgaaagcgc gtgattgggt
     3781 gaaagcgcgc atggccaaag cgggcatggt ggaggcggca taatgcaaaa gctaaaacag
     3841 caggtgtttg aagccaacat ggatctgccg cgctatgggc tggtgacctt tacctggggc
     3901 aacgtcagcg ctatcgaccg cgaacgcggg ctggtggtga tcaagcccag cggcgtcgcc
     3961 tacgaaacca tgaaagcaga cgatatggtg gtggtcgata tgagcggtaa ggtggtggaa
     4021 ggggaatatc gtccgtcttc cgacacagcg acgcatcttg aactctaccg tcgttacccg
     4081 tcgcttggcg gcattgtcca tacccactca actcatgcta ccgcatgggc gcaggcgggg
     4141 ctggcgatcc cggcgttagg caccacgcac gccgactact tctttggcga cattccgtgt
     4201 acgcgcgggt taagcgaaga agaggtgcag ggcgagtatg aactgaacac cggcaaagtg
     4261 attatcgaaa cgctgggcaa cgccgagccg ctgcatacgc cgggaattgt ggtgtatcag
     4321 cacgggccgt tcgcctgggg gaaagatgct cacgatgcgg tgcataacgc ggtggtgatg
     4381 gaagaagtgg cgaaaatggc gtggattgcc cgcggcatta acccacaact caatcacatc
     4441 gacagctacc tgatgaataa acacttcatg cgtaaacacg gtcctaacgc ttattacggg
     4501 cagaagtaga acacgcgctg cggaaatttc cttcctcggg agataactgg tctaattccg
     4561 cagccgtttt tcaaaaaaag ccccctgcga agggggcaaa gcaaactatg gcaatgtttc
     4621 gttggttata cctggtgcta gcgataaata tccgcgctgg cgtgcatatt gccgttactc
     4681 cccggttccc gcatcagaat tacgtggtag tacgttgcgc cttgcgcatc tgtttcttca
     4741 tttaatgcct gacgtgcttc gctttcagtg gcgaagttat gattgatata aatcacgcct
     4801 aagctttgca catcgtccat atttctggcc tggtggttat taatttcaat ggctgcccat
     4861 gtatttgcac ttagcaaaag cacagccaga agggctaaaa cacgactgaa catagatacc
     4921 tcctcgacgg ctgactttgt gtgctctcct ctgtgatgat cttctgattt aattttaatc
     4981 aatgataaag aagttgatgg tgaccatttc tgatgcagtt gttcaaaaaa acaccatgat
     5041 gaagtgtgat gaacttcaaa tcagcgtgtt agtccacttt ggtgaaagat gagatttatt
     5101 tgtcggctgg ccttgagttt agcggggtat gtcagtacta taacgcgtca tttttcagcc
     5161 gacctttaac acgttccttg cctccccggg attcggctga cccagacagg aggctgaata
     5221 atccgtaagg agcaattcga tgcgtcatta cgaaatcgtt tttatggtcc atcctgacca
     5281 gagcgaacag gttccgggca tgatcgagcg ctacactgct gccatcactg gtgcagaagg
     5341 caagatccac cgtctggaag actggggccg ccgtcagctg gcttacccga tcaacaaact
     5401 gcacaaagct cactacgttc tgatgaacgt tgaagctccg caggaagtga tcgatgagct
     5461 ggaaactacc ttccgcttca acgatgccgt tatccgcagc atggttatgc gtaccaagca
     5521 cgctgttacc gaagcatctc cgatggttaa agcgaaagac gagcgccgtg agcgtcgcga
     5581 tgatttcgca aacgaaaccg ctgatgatgc tgaagctggg gattctgaag agtaatttct
     5641 gatgaccaac cgtctggtgt tgtccggcac cgtgtgcagg actccccttc gaaaggtcag
     5701 tccatcagga attcctcact gccagttcgt gcttgagcat cgttctgtgc aggaggaagc
     5761 cggttttcac cggcaggcgt ggtgccaaat gcccgttatt gttagcggac acgaaaacca
     5821 ggccattact cacagtataa cggtcggcag tcgcataacc gttcaggggt tcatttcatg
     5881 ccacaaggca aagaacggac tgagcaaaat ggttttgcat gccgagcaga ttgaattgat
     5941 agattctgga gactagccat atggcacgtt atttccgtcg tcgcaagttc tgccgtttca
     6001 ccgcggaagg cgttcaagag atcgactata aagatatcgc tacgctgaaa aactacatca
     6061 ccgaaagcgg taagattgtc ccaagccgta tcaccggtac ccgtgcaaaa taccagcgtc
     6121 agctggctcg cgctatcaaa cgcgctcgct acctgtccct gctgccgtac actgatcgcc
     6181 atcagtaatc ggtcacggtc cattaatacg actttgagag gataaggtaa tgcaagttat
     6241 tctgcttgat aaagtagcaa acctgggtag cctgggtgat caggtaaacg ttaaagcggg
     6301 ctatgctcgt aacttcctgg taccgcaggg taaagctgtt ccagctacca agaaaaacat
     6361 tgaattcttc gaagcacgtc gcgctgaact ggaagctaaa ctggctgagg ttctggcagc
     6421 tgctaatgct cgcgctgaga aaatcaatgc actggaaact gttaccatcg cgtctaaagc
     6481 tggcgacgaa ggtaaactgt tcggttccat cggtactcgc gacatcgctg acgctgtaac
     6541 tgcagctggc gttgaagtgg ctaagagcga agttcgtctg ccgaacggcg ttctgcgtac
     6601 cactggcgaa cacgaagtga gcttccaggt tcacagcgaa gtattcgcga aagtgatcgt
     6661 aaacgtagta gctgaataat tcgttattca acgagacgta aaaacgccga ccagtggtcg
     6721 gcgttttgct ttctattttt cgtcaggtat tagtttcgca agtagatcca ttcaccattc
     6781 tcttgtgtcc ataagtcagg atggtttgct ggcgttagat acatacgagc gcaactgatg
     6841 tctcgctcct gcggcactcg ctcgaaaccc aaacccgagt agattgcggc tgaacttgat
     6901 aaggaacgca gaccaatttt caaatcatgg ggtaaatcag aatacgcgtt tagcaatgca
     6961 gcaatgatga atccgagggc attagaaacg cctgtaacag tgcgaagttg gtcgatataa
     7021 agatcgattg ttggtagttc agatgaccag caattaggca tttgggcagg aagggttaca
     7081 acgtatccta tcgtttggcc ggtatagtct ggagtaccat aaccaggtcc caaaaccact
     7141 tcgtgtattg tgtatttact gttgggttcg aaggattcga catcttgcaa taaatatttc
     7201 agttctgcgg ttaacctcgc acccgcgtaa gctttattgt atttctgggc gtgaatatta
     7261 tctaatgtag cttcatggtt ccatccaaaa gaattatcac cattcattgt gattccatcg
     7321 taagcattac acctcgtcgc taacttattg aatgcttgcc agtctgagag atctagtttg
     7381 tggatctcaa cagtacgtct gccatcgcaa ttatgatctc ttccacgatt tgacagtaat
     7441 gtagcgcgtg cgtattgatg aaattttagt tttgctggtt tgtcggtgga cgaaaaacga
     7501 ggtaaatatg cggggaatga ataaatagtt gttggtaatg tcattttatt gttgcct
//
LOCUS       DAZUCE010000232         7531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZUCE010000232 DAZUCE010000000
VERSION     DAZUCE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.232"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            826..1710
                     /locus_tag="ACHY03_004292"
     CDS             826..1710
                     /locus_tag="ACHY03_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFS3907459.1"
                     /translation="MGMLEREMKNLARQAGGSHKTVHDRIAMAGRFCERLMELNVQIR
                     YVHHLKARHIEAYIQMRLAQGIKKQTLHNETAAIRKILTQAGREKLAQSERISNKSLG
                     LAGVSRNGTRKAITPEYYQQMADTARLKDAGLAAALELARLMGLRSQEAVRCSCSLQT
                     WRQSLERGDTRLTIVFGTKGGRPRDTLIQDTGAVKKSLDNAIAVAGLRNGRLIDAASL
                     KQAMKYWRNQTLRMGLTGKYSPHSLRYAWAQDDIRRYLAQGFSEKEALAMVAMDLGHG
                     DGRGRWVKQVYAHGWTEE"
     gene            2309..2602
                     /locus_tag="ACHY03_004293"
                     /pseudo
     CDS             2309..2602
                     /locus_tag="ACHY03_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728926.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
     gene            2949..4352
                     /locus_tag="ACHY03_004294"
     CDS             2949..4352
                     /locus_tag="ACHY03_004294"
                     /inference="COORDINATES: protein motif:HMM:NF036729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine phosphorylase"
                     /protein_id="HFS3907460.1"
                     /translation="MTNSLMLASSHILLAGSISNATENALIDRAHEFVDAFVEEVLKA
                     GGGFVIYVAAEPVNSDNKPLLFDWTVARAIDKLLPDDSQQVRLKIVASQERLQSKANP
                     EQRRLLLGMIARGVAELVPLEEEVLTGGNVGDEQIEHATAMVALGGGKGVLDRARKMS
                     KRQLPVLPLDLQLGANSEDGKGAIGILKSFQTTPLTYMPNSGNKVIKTLTALSLQDPV
                     MSISDISKRIVKIFYEEELARVQALPPDVLVLTALDVELAAAKQAFGIAEDAEHTTTE
                     NGLHIWKTPVSKRSGKTASCVLACFAGAGNVDAASVTSMLLGELRPANVMMLGIAAGL
                     RDKCALGEVVIAERVVAYEGATFLEEGRVENRPEISRLSMRVRQDVTFPTGRHSNFAL
                     QKVIEHWVLNFPTKWRRVPSSKESCRRQPLLLVVKSFFEIPINSWACVSFTVRLKLQK
                     WKAQDFSLHAPTLANQC"
     gene            4262..4477
                     /locus_tag="ACHY03_004295"
     CDS             4262..4477
                     /locus_tag="ACHY03_004295"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907461.1"
                     /translation="MRELHGKTEVAEMEGAGLFAACANFGKPVLMIRGISDFGDSKKD
                     NRFHLLAAKAAAAVTVDYIAHGMTLQD"
     gene            4531..5478
                     /locus_tag="ACHY03_004296"
     CDS             4531..5478
                     /locus_tag="ACHY03_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CBASS oligonucleotide cyclase"
                     /protein_id="HFS3907462.1"
                     /translation="MSLSNTALEYFDHNVLRLPGEKRKEYHAQVDNLVSELKKRITDK
                     SKLKVKKVVKAGSFAKYTILRKIDDYPTDVDVVFYITGVEENSKSYEVLCNRIYDLLI
                     EIYPTKKVEDFEIQRRAAKVTFVKSGLEVDVVPVLQHSTLADHGWQYDIQSGARNLTC
                     APCHIQFIRTRKDKDKHFRTLVRLAKRWKHFHDIPGLKSFHIELILAHLVDTDGAAEN
                     IEKRFREFLVYIARTKLGERIDFPENEGKTSVSFSDPVVIIDPASPENNVASRITKDE
                     QEQIAKAAEAAWEAATYASTKNDDDLWKEIFGGRFKTKD"
     gene            5484..5972
                     /locus_tag="ACHY03_004297"
     CDS             5484..5972
                     /locus_tag="ACHY03_004297"
                     /inference="COORDINATES: protein motif:HMM:NF037203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907463.1"
                     /translation="MSTYSSTSTYTVADVEEVMCSVKADLIMIAGSTKAMTESDAANY
                     AHDIEVLAKKNYLKFADVTLMNGSTEVRAVKYEFQTEGATGTGRPGGVLWPLTPKDKG
                     GWVRIHLRYTDSSSAEERAKLPLKISWVPASTDVGHKGLASSAGRNYSSNGFGTNRED
                     FS"
     gene            5969..6865
                     /locus_tag="ACHY03_004298"
     CDS             5969..6865
                     /locus_tag="ACHY03_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3907464.1"
                     /translation="MSNSSVFEVKTNLPTAQLDVQAQKLLGFEGRYTRVKKRLQLILQ
                     ANELDRWSQTYHKRKLAVIGLLDDQYPLAIFHGDVGTGKTANAEAIANRLARDAKLED
                     AILFKLSNKVRGTGKVGEMGTLITQAFEDITRSIGPGKGRAFLIIDEGDSLGASRSQD
                     HSHHEDKVGVNTLIQSIDGMRKHSGRIFVILCTNRLRALDAAVIRRASIVEEFTRPSD
                     TERHELFTKDLGDMGFSPIDVLKLVKATAATDTKPAWTYSDIRTRLYPAAVSLAFPNS
                     PLTIEHFYQAVAELGPSPVMAG"
     gene            6891..7442
                     /locus_tag="ACHY03_004299"
     CDS             6891..7442
                     /locus_tag="ACHY03_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFS3907465.1"
                     /translation="MSKKNEVFISVDIETSGPIVGEHSMLTLGACLAYQPEVSFSVML
                     KPISNESVADALEVTGLTMAEAEKKGLPPVEAMSQFAAWISNNLPKDSTAVFVGLNAP
                     FDWGFVNYYFLKYLENNPFGFTALDIKALFMGVMGCSWYETRSSAIDKIVSPSQKGDH
                     DALHDAKYQAELFRLVYELSKKK"
BASE COUNT         2047 a         1722 c         1961 g         1801 t
ORIGIN      
        1 tttctataaa ttcaagggga aactcaattt ccagaaaaat gagtttcccc tttctgcgcc
       61 agaaattacg gtgtaatctc tctcctgcaa taagctgtcg gttgacagcc cctttggctt
      121 catggtctgc aagaagcccc tgatttatca ggacgaccag cccactaccg gtacgacctc
      181 cgcaataccg gaacgcgaaa gcgtttgagg ctaagagtac atacacctgg acggatccat
      241 cgtcatcgct taacggcaac agccttcacc ggctacacgg cttctctttg ccggaatttt
      301 cctggcccac ttataccctg ctgattacca gcgcccttta catgacgggc tgcggcctgc
      361 atttgccgaa ggcatttgct gccgcgtgac cgtcatcctg caccagagtg ttaattttca
      421 gctcctgcgc cagaccaggg cgtcagtcgc tcacgcagag tgagagactg acgcccgcag
      481 aaatctcaat ctgcaccagc catatcaact gctccagcca caggcgagct tctgacgtcg
      541 cttttcagca tttatctcaa ccattcattc acaaaaggag aacatgagcc agtaccggag
      601 gggaccacca ggacggtccg ggggatgacc agctaacgct ggcggctgct ctgagagcac
      661 aaccgatacc ccgcaaaacc tcaacagccg tcgccccggc gctcaggtac tgctcgcagc
      721 cccgacaaaa atcaggggct atcggcctac gcgccgggga aagctgtatc cgttgtctgt
      781 tgtggttttg tgaaaatcag gagcaaaggt aagaggaaaa gcaaaatggg tatgctggaa
      841 agagagatga aaaacctggc gcggcaggct ggcgggtcgc acaagactgt ccacgaccgt
      901 atcgccatgg cggggcgttt ctgcgaacga ctgatggaac tgaacgttca gatacgttat
      961 gtccaccatt taaaggccag gcatattgag gcgtatattc agatgcggct ggcgcagggt
     1021 attaagaaac agacgctgca taacgaaacg gcggctatcc gtaaaatact gacgcaggcc
     1081 gggcgcgaaa aactggcgca gagtgagcgg atatcgaata aatctctggg gctggctggc
     1141 gtatcccgca atggtacgcg gaaagccatc acgccggaat attatcaaca gatggcggac
     1201 accgcccggc tgaaagatgc ggggctggcg gcagcgctgg agctggcgcg gctgatggga
     1261 ctgcgctctc aggaggcggt acgctgttca tgttcattgc agacgtggcg gcagtcgctt
     1321 gagcgtggcg atacccggct aaccatcgtg tttggcacca aaggcgggcg gccccgcgat
     1381 acccttattc aggatactgg cgcagttaaa aagtcgctgg ataatgccat tgcggtggca
     1441 ggactgcgca acggcaggct gattgatgcg gcgagcctta aacaggcaat gaaatactgg
     1501 cgcaaccaga ctttgcgtat ggggctgacg ggcaaatact ccccgcactc actgcgctac
     1561 gcctgggcgc aggacgatat ccgccggtat ctggcgcagg ggttcagcga aaaagaagcg
     1621 ctggcaatgg tggcgatgga cctggggcat ggcgacgggc gcggtcgctg ggtgaagcag
     1681 gtgtatgcgc acggctggac ggaggaatga cggctttcat gaatgacgca gaataaattg
     1741 ttgcgcagta acggtagttg tttttctgat gcaataatca ggaaaataaa atcgggtaac
     1801 accgttactg cataacaata ttacgctgat gcactctttg gcgagcgagt gttgatggtg
     1861 cagattcaga tcagagataa tttatctggt gcaaactcca taacacatat caggataaac
     1921 tcactgtgcc agaaagggaa agggcataaa ggttaaagga gctaagggta acaggtcttg
     1981 ccctgaaagg gccaaaaacc aaagggctca cctcgccgct tcaggtcggg gggccttcgt
     2041 atatctgtcg gcacagagcc ggggattatc agtcagtcgc aactctaagt tccggcccca
     2101 ataaccgggt gatgcggatt tctttttgtt tacagcactt ttcggttcac ctgtcgttgc
     2161 agttacggtg gtatttacgt tgttccctgt gatgctctgg ctgaccagtt ggaaagaagc
     2221 gctccggatc tccgagtcgg tgagtgtctg gcagcgaggc tgtcccaaag caaaccgttg
     2281 ttgatatccc cgcatggctt gccagtggaa aaaagcgctg gagaatgcgt tagctgttgc
     2341 agaaagtcac aataaccggc tgatcgacaa gcccgatctg aaaagcgcga tggactactg
     2401 gcacaatcag gcaaaacgaa tcggcctgac tggcgcatat tctccgcatt cgctgcgtta
     2461 cgcgtgggcg cgggatgcca tacatcatta tctggcgcag ggagtcagca ggaaagaggc
     2521 actggcaatg gtggcgatgg acttggggca tggcgacgga cgtgggcgct gggttaaaca
     2581 ggtgtatggg cgtgaggaat agcattttca tgaatgatac gagataaaat gttacttcgt
     2641 gacaacatcc ttcactgctt ctctttgtgc cgagcatgac gcacaaggta aaggtacttt
     2701 taaggaaggc ataggtgtgt ttatccctca gcgcccctcg ctcctcaatt acagctgtat
     2761 cagacgcgtc tcggctaatt atccagaatc aatgaaaatt tatacagtat gccccttgaa
     2821 atagagttaa tcgttcccac ttaattacca caatgtggcc gtggactaat tcgaccgcac
     2881 taaagagaat ggatcgtctt acgcgtagaa atggcgcgta ggcatactga attcaagtga
     2941 gactactaat gacaaattcc ctgatgctgg cgtcaagcca catccttctc gctggtagca
     3001 tttcaaatgc gacagaaaat gcgcttatcg accgggcgca cgaatttgtc gatgctttcg
     3061 ttgaagaggt acttaaggct ggtggtggct ttgttattta tgtggcagca gaacctgtta
     3121 actcagataa caaacctctg ttgttcgatt ggaccgttgc ccgggccatt gacaaattac
     3181 tgccagatga cagccaacag gttcgcctaa agattgtagc ctcgcaggag cgtctccaat
     3241 ccaaagccaa ccctgagcaa cgcaggttgc tcttaggaat gattgctcga ggtgttgccg
     3301 aactagttcc tctcgaagag gaggtgttga ctggtggtaa tgtcggtgat gaacagattg
     3361 aacatgcaac ggcaatggtg gcacttggtg gcgggaaagg cgttctggac cgtgcgcgta
     3421 agatgtccaa aaggcagtta cctgtactgc cactcgattt gcaactgggt gcaaatagcg
     3481 aagacggcaa aggtgcaatt gggatcttaa agagtttcca aaccactcct ctaacataca
     3541 tgcctaatag cggtaataag gttatcaaga ctctgaccgc gctatccctt caagatcccg
     3601 ttatgagcat atccgatata agtaagcgca tcgtcaaaat tttctacgag gaagaactcg
     3661 ccagggtcca agctttgccc cccgatgttc tagtactgac agctttggat gtggagttgg
     3721 ctgctgccaa acaagcattc ggtatcgcag aagatgctga gcatacaact acggagaacg
     3781 gtctccatat atggaaaact cctgtgtcaa aacgtagtgg caagacggct agctgtgtgt
     3841 tggcctgttt tgctggtgcg ggtaacgtag atgctgcttc ggtgacttct atgctcttgg
     3901 gggaactcag accagcgaac gtaatgatgt tgggaatcgc tgcgggtcta cgggataagt
     3961 gtgctctggg tgaagtcgtc atcgctgagc gagttgtcgc ttatgaaggt gcgacttttc
     4021 ttgaagaggg cagagtcgaa aatcgtccgg aaattagcag attaagtatg cgagttcgcc
     4081 aagatgttac ctttccaaca gggcgacact cgaatttcgc cttacagaaa gttatagaac
     4141 actgggtatt gaatttcccg accaagtgga ggcgggtccc gtcatccaag gagtcatgcc
     4201 gaagacagcc actgttgcta gtggtgaaaa gcttcttcga gatcccgata aattcttggg
     4261 catgcgtgag cttcacggta agactgaagt tgcagaaatg gaaggcgcag gacttttcgc
     4321 tgcatgcgcc aactttggca aaccagtgct gatgattcgg ggtattagcg attttggtga
     4381 ttcgaaaaag gacaaccgat ttcatctctt ggcagcgaaa gctgctgcgg cagttactgt
     4441 cgattatata gcacacggca tgacgttaca ggattaaaag tcaagccatt cctctagttg
     4501 atgtatgcct gtatcaggca tggagaaata atgtctctat caaatacggc tcttgaatat
     4561 tttgaccata acgtgctgcg cctccctgga gagaaacgca aggaatatca cgcgcaggtc
     4621 gacaacctcg taagcgagct aaaaaaacgc attaccgata agtcaaaact caaagtcaag
     4681 aaggtagtga aagctggttc attcgctaaa tacactattc tgcgcaaaat tgacgactat
     4741 ccaacggatg ttgatgttgt tttttacatc accggtgttg aagaaaacag caagtcctac
     4801 gaagttctgt gcaacaggat ctacgatttg ttgatagaga tctacccaac caaaaaagtt
     4861 gaggacttcg aaatccagcg tcgtgcagca aaggtaacat tcgtcaagag tggtcttgaa
     4921 gtagatgttg tccctgttct gcaacacagc actctggcag accacggttg gcaatacgat
     4981 atccaaagcg gagccaggaa ccttacctgc gcaccgtgcc acatccaatt tatccgtaca
     5041 agaaaggata aggataagca ctttcgcacg ttagtgcgtt tggcaaaacg ctggaagcat
     5101 ttccacgata tcccgggtct gaagtccttc cacatcgaac tgattctggc ccatttagtt
     5161 gatacggatg gcgcagcaga aaatatcgag aaacgtttcc gggaattctt agtttatatt
     5221 gcccgtacca aactgggcga acgtatcgac ttcccagaaa acgaaggcaa gacatctgtt
     5281 agcttcagtg accctgttgt gatcattgac ccggctagtc cagaaaacaa tgtggcatct
     5341 cgtataacga aagacgaaca agaacaaata gctaaagctg ctgaagctgc ctgggaagca
     5401 gcaacttatg catctactaa aaacgatgat gatctctgga aagagatttt cggtggccgt
     5461 ttcaagacca aggattaaaa ataatgagca cttattcaag cactagcacc tacacggttg
     5521 ccgatgttga agaggtcatg tgcagtgtca aggctgattt gattatgatt gctggctcga
     5581 ccaaggcgat gactgagtcc gatgcagcaa actatgccca cgatatcgaa gtcctggcca
     5641 agaaaaacta tcttaaattc gctgatgtga cgcttatgaa cggttccact gaagtccgcg
     5701 cagtcaaata tgagtttcag actgaaggtg caacgggaac aggaaggccc ggcggtgtat
     5761 tgtggccgct gacaccaaaa gataaaggcg ggtgggttcg tatccatttg cgctataccg
     5821 actcgtcatc ggcggaagaa cgcgcgaagc ttcctctgaa gatatcttgg gtgccagcct
     5881 caaccgatgt cggtcacaaa ggactagcct cttctgctgg tcgtaactat agcagtaatg
     5941 gttttggcac aaacagagag gatttttcgt gagtaactca tcagtattcg aagtaaaaac
     6001 taaccttcca acagcccaac ttgatgttca agcacagaaa ttattgggtt ttgaggggcg
     6061 ctatacgcga gttaaaaaac gccttcagct cattttacag gctaatgaac tggaccgatg
     6121 gagccagaca tatcacaaaa gaaaactggc agtaattgga ctgctggatg accagtaccc
     6181 tttggctatt ttccatggtg atgtaggcac aggcaaaaca gctaacgccg aagcaatagc
     6241 taaccgttta gcccgtgatg caaaactcga agatgccatt ctttttaagc tgagtaacaa
     6301 agtgcgcgga acaggcaaag taggcgaaat gggaactttg attactcaag ctttcgaaga
     6361 tattaccaga tctatcgggc cgggcaaggg gcgcgctttt ctaatcattg atgaaggtga
     6421 ttctttggga gcatcccgta gccaggatca tagtcatcac gaagataaag taggtgtcaa
     6481 cactcttatt caatctattg atggtatgcg taaacatagt ggacgtattt ttgtcatact
     6541 ttgtacaaat cggctgagag cccttgacgc tgcagttatt cgccgcgctt caattgtgga
     6601 agagttcact cgaccttccg atacagaacg ccatgaattg ttcacaaaag atctaggtga
     6661 tatgggattt tccccaatcg atgtgctaaa actggttaaa gcgactgcag cgacagatac
     6721 taaaccagct tggacgtact ctgatatcag aacgcgtctt tatccagctg ctgtatcttt
     6781 agcatttcct aattcgccac taacaattga acatttttac caagcggttg cggagttagg
     6841 gccatcgccg gtaatggcgg gataaattaa taatgattgc cggaggtcta atgagcaaaa
     6901 aaaatgaagt ttttatctcg gtcgacatcg aaacttcagg tcctattgtc ggtgaacaca
     6961 gcatgttgac gttaggggcg tgcttggcat atcagcccga agtatccttt tcggtaatgt
     7021 tgaaaccgat tagcaacgaa tcggttgctg atgccttaga agtcacaggg ttgacaatgg
     7081 cagaagcaga aaaaaaaggg ttgccgccag ttgaagccat gtcacagttt gctgcatgga
     7141 tctccaataa cttacctaaa gatagcacag cagtgtttgt aggattaaat gcgcctttcg
     7201 actgggggtt tgtgaactac tattttctga aatatctaga aaataaccca tttggtttca
     7261 ctgcgttgga tatcaaggcc cttttcatgg gagtcatggg atgttcatgg tatgaaacca
     7321 gatccagtgc gattgataaa attgtgagtc caagccaaaa gggagatcac gatgctcttc
     7381 acgacgcaaa atatcaagct gaactattca ggttagttta cgaattaagc aaaaaaaaat
     7441 agttaatatt agtcaaatat ctgcgtaggg tatggtcggt tatagcctgc gcagataact
     7501 gacttgaatc agccccagaa taactaaaat t
//
LOCUS       DAZUCE010000233         7397 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZUCE010000233 DAZUCE010000000
VERSION     DAZUCE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.233"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            170..3982
                     /locus_tag="ACHY03_004300"
     CDS             170..3982
                     /locus_tag="ACHY03_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HFS3907466.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESAALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINAFGNIENRDGNSFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRFIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNTL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVNNNNSS
                     IIAENGPLNLLSEGTLDNTRALLSSGADAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDSSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINAQNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4071..5690
                     /locus_tag="ACHY03_004301"
     CDS             4071..5690
                     /locus_tag="ACHY03_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFS3907467.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLSDLPQEKNCFIINRVEVEDNFLGGQLISEVHKAVTGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTAVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGTSEYLSAKATRTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQIKNGYYNTAFFAQYAPRELTLQDQITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGAAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            5706..6074
                     /gene="acpS"
                     /locus_tag="ACHY03_004302"
     CDS             5706..6074
                     /gene="acpS"
                     /locus_tag="ACHY03_004302"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFS3907468.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTAHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIEQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            6088..6849
                     /locus_tag="ACHY03_004303"
     CDS             6088..6849
                     /locus_tag="ACHY03_004303"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFS3907469.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNYDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIEREAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            6853..>7397
                     /locus_tag="ACHY03_004304"
     CDS             6853..>7397
                     /locus_tag="ACHY03_004304"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HFS3907470.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRET"
BASE COUNT         2064 a         1583 c         1896 g         1854 t
ORIGIN      
        1 aaacaataac tcttagttgt ttttttgagt ccttatttca gcttattttt cgtgattttt
       61 tattgcgcca ggtgatagga tttgccgcaa tcatttttgc tgatattccg tgagtcatca
      121 aaagtgatct gcatgtccaa aatgaatagc atcaataagg aaaacgttag tggcgatgat
      181 aaatttaagt aaggaagcaa cggtgggtaa agcattaacc cctattgcta tacttatgat
      241 gttgtctttt cctgtagctt ctcaagcggc gggattagtc ataaaaaatg gaacggtata
      301 taacgccaat ggtgtgccag tcgttgacat caacaaacct aacggtagcg gtttatctca
      361 taatatctgg gataacctaa acgttgataa aaatggtgtc gttttcaata acagcgctaa
      421 tgaatccagt acttcacttg ccggaaatat tcagggaaac agtaatctga cctccgggtc
      481 ggctaaggtg atcctgaatg aggttacttc caaaaatcct tcaaccatta atgggatgat
      541 ggaagttgca ggggataaag cggatctgat tattgccaac ccgaatggta ttactgtaaa
      601 cggtggcggt tcaatcaata caggtaaact gaccttaacc accgggacgc cggatatcca
      661 ggatgacaag ctggccggtt actccgtgaa cggcggtacc attacgctcg gtaaactgga
      721 taacgccagc ccgacagaaa ttctgtcccg taacgtggtg gttaacggca aagtgtctgc
      781 cgatgagctg aacgttgttg ctggcaataa ctatgttaat gccgcaggcc aggtgaccgg
      841 tagcgtatcc gccacgggtt cccgtaacgg ttacagcgta gatgttgcca aactgggcgg
      901 aatgtatgcg aacaaaatca gtctggtcag caccgagaaa ggtgtggggg ttcgcaacct
      961 cggcgttatt gctggggggg ttaatggtgt cagcatcgat tccaaaggta acctgttaaa
     1021 cagtaacgcc cagattcagt ctgcaagcac gatcaacctg acaacaaatg gtactctgga
     1081 taacaccacc ggtacggtga catctgtagg cactatctcg cttaatacca acaagaatac
     1141 tatcgtgaat acccgtgcgg gtaacatctc tacgatgggc gatatctacg ttaacagcgg
     1201 tacgattgac aatactaacg gcaagcttgc ggctgcagga atgctggcgg ttgataccaa
     1261 taacgccacg ctgattaact ctggtaaagg gagttctgtc gggattgaag cggggctcgt
     1321 ggcgctgaaa accggaacgc tcaacaacag caatggtcag attcgcggtg gctatgtggg
     1381 tcttgaatcc gctgcgctga ataacaacaa cggtgatatc cagaccaccg gcgatatcgc
     1441 cattatcagt aacggtaatg tggataacaa caaaggtctg atccgttcgt ccaccgggca
     1501 tatcgttatt ggcgcggcag gtagcgtaaa taatggttca accaaaaccg ccgataccgg
     1561 cagttctgac tctctgggca ttattgcaga taccggcgta gaaattggtg cgaacaacat
     1621 caataacaac ggcggacaga ttgcgtctaa tggcaacgtc tccctgtcaa gttacagcac
     1681 gatcgacgac tatgcgggca aaattctgtc caacagcaaa gtgattatca agggaagctc
     1741 actacgtaac gataccggtg ggatcagcgg taagcagggc attgaagtcg ccgttggcgg
     1801 cagcctgact aataacattg gcgtgattag ctctgaagag ggtgatatct ccctgttagc
     1861 caactcagtg gacaaccacg gcggcttcat gatggggcag aacatcacga tggagtcgat
     1921 gtctggcgtc aataacaaca ccgcgctgat cgtggccagc aaaaaactga agataaacgc
     1981 gttcggtaat attgaaaacc gcgatggcaa tagcttcggt aatgcatatg gtctgtactt
     2041 cggcatgcct cagcaaacgg gtggaatggt cggcaaggaa ggcatcgagc tttccgggca
     2101 gaatatctat aacaacaaca gtcgtttcat cgctgaggat ggtcctctga ctctgcaggc
     2161 acagaatacg ttcgacaaca cgcgtgctct ggtcaccagc ggggcggatg catctattca
     2221 ggttggcgga acgtattaca acaactacgc taccacctgg agcgcgggta acctggatat
     2281 cgacgcgacc acactgcaaa acagcagtag cggtacgatg atcgataaca atgcgaccgg
     2341 gttcatagca tctgataaaa acctgtcact ggaagtggtg aataccctta ccaactatgg
     2401 ctggatcagc ggtaaaggtg atgttgatgt cacggtgaat aacggaaacc tgtacaaccg
     2461 caatactatt gcggctgaaa aggggctgga cattgcagcg ctgaacggta ttgaaaactg
     2521 gaaggatatt tctgctggcg gcgacctgac gatgaacacc aatcgccatg tgaccaacaa
     2581 ctccaacagc aatatggtgg ggcagaatat cgttattaac gcggttaacg atatcaacaa
     2641 ccgtggcaac attgtcagtg acgctgactt gaacgtgacg accaaaggca acctgtataa
     2701 ctatctctat atggtagggt atggggatat cgcattgtcg gcaaatagcg tggcgaacaa
     2761 taacgcgacc atcgaagcga caggcgatct gattatcgat tcgaagggta acgtgggtaa
     2821 caaccgcggt aatctgcatg cgttgaacgg cgtgttgtct gttaaaggca acaatctgaa
     2881 caacgataac ggtgaaattc gtggttatgg cgatgtcacg ctggcactga cgggcaacta
     2941 cgacagctat aagggttcgc tgacctctga aacgggcgac gtgactctga cggcgaacat
     3001 cgtagacaac gcctatggtt tgattgccgg tgagaatgtt tctgtcgatg ctaaatcgac
     3061 gatttacaac aacactgcgc tgatcgcggc gaataaaaag ctagttatta acgctggcgg
     3121 caacctcgaa aaccgcgacg ggaataactt cctgcgtaat aacggcgcgc tgtttgggat
     3181 caccgacaac gttggcggca tcgtaggtaa agaaggtgtc acgctttctg ctcagaatgt
     3241 caacaacaat aacagcagca tcatcgctga aaatggtccg cttaatctgc tgtccgaagg
     3301 aacgctggat aatacccgcg cgctcctcag cagtggggct gatgccatca tccgtgcggc
     3361 agggacgttc tacaacaact atgccaccac gtacagcgcc ggtaatctcg acgtttatgc
     3421 ggcgtcgttg aacaacgcca gcgatggtcg cctggaagac aataccgcca cgggcgtgat
     3481 tgcgtctgac aaaaatctgg atctgagcgt tgatagcagc gtcactaact atggttggat
     3541 cagcggtaaa ggagatgtgc atttcaatgt tctgaaaggc acgctgtata accgtaatgc
     3601 catcgcggcg gataacgcgc tgaccattaa tgcccagaac ggtgttgaga actttaaaga
     3661 catcgtggcg ggaaccgcgc tgactattga tacgcagaag tatgttacca acaacagcaa
     3721 cagtaatatg ttggggcaaa ctatcgcgat caatgccgtg aatgacatta ataaccgtgg
     3781 aaatattgta ggtgattatt ctctgggggt taaaaccacc ggtaatattt ataactacct
     3841 caatatgctg agttatggtg tcgctggcgt atcggcaaat aaggttacga acagcggtaa
     3901 agacgctgtt ctcggtggct tctacggttt agcgttagaa gcaaacgaaa ctgataacac
     3961 cggtactatt gtcggcatgt aagatgtaac caaagacagc agtgactttt aatattaatg
     4021 cgtcatgtca cgcaggggtt gctgctgtct ttatttagtt tgagaagata atgcaattac
     4081 gatttaattt tggtttgctg ctcattcttt gttgttttca ggttgcagcg aaagacagtc
     4141 ttgataattt gatcaagcag caaaaaaatg cagatgaaag cacgttacgc gcgcataaat
     4201 tagaaaaaaa agatgtttac tcaaatgcac aacgtaatgc gtttactctg agtgatctgc
     4261 cgcaggaaaa gaattgcttt atcatcaatc gggttgaagt ggaagataac tttctggggg
     4321 ggcagttgat ttctgaagta cataaagcgg ttaccgggcg ttgtgtcggt agtgctggtg
     4381 tgcaaaaaat tgctgctgcc ttgcaggatt attatattaa tgccggatat atcacaacgc
     4441 gaattagtat cccttcgcag gatatttcta aaggcaagct acgtttccag gttaatgccg
     4501 ggaagataga aaaaacgatt gtggaaggta atgacatcag tgaatggatg ttaccttttc
     4561 agcgtaacga tattctcaat attcgcgata ttgaacaggg gctggaaaat cttcagcgag
     4621 tacctgacgt tgatgtgaaa attaatattg aaccgggaac acgggatggt tacagcatcg
     4681 ttcatattga tacacaccgg gggaaaaact ggagcgttcg ggcaagttac aataattggg
     4741 gcgatgaaga aacgggccgt taccagacaa ccgcggtggg ttatctgttt aatccagcga
     4801 aaatggggga tctgttttat ctcgccggta cgcgtagtac taccggacag tatgaaaacg
     4861 tcagtagtta ttattccatt ccggtggggt actgggagta cggctttttc tatagcaaaa
     4921 gtaaatcgca gcaggtgatt ccgctttcgt acatctcgct ggattatgtt ggcaccagcg
     4981 aatacttgag cgcaaaggcg acgcgtaccg tttatcgcga taaaacaaaa aaattcgccg
     5041 ggagtgcaga actcattcga cgaaagtcga attacacgct taatggtgag gagctgacat
     5101 tacaggcgcg ggatatggga aatatcaagt tggggctaaa ttataagcaa cagttacccg
     5161 gcgcgttctg ggattcaacc ctctcgtggc aacgcttttt aacctggttc ggtggtgaaa
     5221 aaacgccgga tatgcaatat ggcgacgtta gcccggtgag caatatcttt aattttgagg
     5281 gtaattacac ccgacaaata aagaatggat attacaatac cgcttttttt gcccagtacg
     5341 caccgcgtga actgactttg caagatcaaa ttacagttgg cgatcgttgg agtattcgcg
     5401 ggtttgaaaa tagcgtaggt ttgtcaggga atgatggttt ttatataaaa aatacgctgg
     5461 catttccttt gcctggaatg aaagctaact attatgccgg tcttgatttt gggcaagtgt
     5521 atcaggacgc ttcttatggc gatgaatcac tggtgggcgc ggcagttggt attgatggaa
     5581 atattaaaag tcttgagtat aacttttccg tttctacccc attaaagtat ccggcgacat
     5641 tagatattga tcgggtgaat gtaaatttta atttttcgta ccaaatgtaa aggataacga
     5701 gactcatgag aatagggaca gatattgttg aaattgccag aataaccaaa gccattatta
     5761 atggagagat ggctttttat gagcgtgttt atactgctca tgagcgctta ctcattaacc
     5821 ctgcagaacc tgattatgaa cgggcctctg gattctgggc ggcgaaagaa gcgttagtta
     5881 aagcgattgg ttgtggtttt cgtgatggcg tacgtttcca ggatattgaa ataaagcatg
     5941 atgcagccaa agcaccttat ttcgttattt ccggaagact acaagaaatt tttatcgagc
     6001 aggggttgca aaataactcc ctgagtattt cgcattgccg tacccacgcc attgcgactg
     6061 tcatcgttta ttaactcaac atatgtgatg aatatgtaca aatataatga tttaaatgaa
     6121 aaaaccgtac tggtgacagg agccagtggt gatattggac tgggtatttg cgaaaaatat
     6181 ctggagcaga actacgatgt ttatgcgctc tataaatcca acgacgtgca attaacggcg
     6241 ctgaaagcaa gccatcctgc aggcgataaa ctgcatattg tgcagtgcga tctcgcctgc
     6301 ccccagagcg tgagcgcatt gtgcgaacag atagagcgcg aagctggcaa gattgatgtg
     6361 ctggtgaata acgcgggcat cgttaaagac agcctgtttg cctcgatgag ctatgaggat
     6421 ttcacccagg ttatcgaaac caacatgttc agcattttcc gtctgacgaa agacgcgctg
     6481 atgttgcttc gtgcggcaga aaatcccgcc attattaacg ttgcctctat tgcagcgctg
     6541 atcccaagcg tcggtcaggc gaattacagc gcgtcaaaag gggcgatcct aggcttcacc
     6601 cgcacgctgg cagcagaaat ggcaccctgg ggcgttcgcg tcaacgcggt cgcaccggga
     6661 atgattgaat cgaaaatggt gaaaaaagta tcccgcgcag tggtccgtgc ggtaaccagt
     6721 accatcccgt tacgccgtct gggtaaatgt gaagaagtgg cgaacaccat cgtcttctta
     6781 agttcctctg cctccagcta cattgtcggc cagaccatcg ttatcgatgg cggcctggtc
     6841 atgcggtgat ttatgtctaa atacacgatt ccttcgaaaa tcttcctcga aatgggcggc
     6901 tggcgtcagc cgctgctgat ggtcgataaa attgcagatt ataaatacgg tgaaaatggc
     6961 tttgtcagtg tcgtgaaaca cgtcacctat aacgaaccgt atctactggg gcatttcccg
     7021 gaagatccga ttatgcccgg ggtgattatt tcggaaatct tcggccaggc cagcgagtat
     7081 ctctctttcc tgaccgacat ttgcgatatc tggcgcgaac gttttgagga agagctgaag
     7141 tcgttacgtg atattcacgc acgcattcac cgccctgaaa tgctggagat tatccgtacc
     7201 cggcgcagcc aggtacgcgg cgtgctggct gctcagaacc tgaaatttaa agatatcgcc
     7261 tatccgggtg attctattga tgttgtcagc aaattggctt tttcggatgc cagtggcttt
     7321 aaacactatt ccgtgaccgc gtacgtcggt aaaaagctga ttagtcaggg caccattatc
     7381 aattttcgtg aaaccaa
//
LOCUS       DAZUCE010000234         7312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZUCE010000234 DAZUCE010000000
VERSION     DAZUCE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.234"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(148..1038)
                     /gene="metF"
                     /locus_tag="ACHY03_004305"
     CDS             complement(148..1038)
                     /gene="metF"
                     /locus_tag="ACHY03_004305"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFS3907471.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            1114..1359
                     /locus_tag="ACHY03_004306"
     CDS             1114..1359
                     /locus_tag="ACHY03_004306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907472.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     LTNHVLIASCRIRRKRFIRPNPMIIQYISLETIDPKT"
     gene            complement(1387..3819)
                     /gene="metL"
                     /locus_tag="ACHY03_004307"
     CDS             complement(1387..3819)
                     /gene="metL"
                     /locus_tag="ACHY03_004307"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFS3907473.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYDIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3822..4982)
                     /gene="metB"
                     /locus_tag="ACHY03_004308"
     CDS             complement(3822..4982)
                     /gene="metB"
                     /locus_tag="ACHY03_004308"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFS3907474.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            5259..5576
                     /gene="metJ"
                     /locus_tag="ACHY03_004309"
     CDS             5259..5576
                     /gene="metJ"
                     /locus_tag="ACHY03_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFS3907475.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5760..6368
                     /gene="yiiX"
                     /locus_tag="ACHY03_004310"
     CDS             5760..6368
                     /gene="yiiX"
                     /locus_tag="ACHY03_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFS3907476.1"
                     /translation="MKYRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSSPQV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            6513..7188
                     /locus_tag="ACHY03_004311"
                     /pseudo
     CDS             6513..7188
                     /locus_tag="ACHY03_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1798 a         2023 c         1827 g         1664 t
ORIGIN      
        1 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgataacct
       61 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
      121 gtgtgatttt cataaaagcc acactattta taaaccaggt cgaaccccca gcgtatggca
      181 aatcgcgtaa ctcatttcag cacggttaag cgtatagaag tggaaatctt tcactccttc
      241 acggcttaaa atcttcacca tatccatggc aatattcgcg ccaaccagtt tgcgggtttc
      301 ggcatcatca tccaggccgt cgaacatttg cgccatccac gccggaatac gcacgttggt
      361 catatcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaattc ccggaataat
      421 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
      481 gaagaagaac tgagtaatcg cgcggttggc tccggcatcg actttgcgtt tcaggttaag
      541 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggagat
      601 atcgaaatct gccacttctt ttaatagcgt caccaggtca gaagcataca tttctggctt
      661 accacttccc ggcggcagat cgccacgcag cgccacgata tgacgaatac cgttattcca
      721 gtagtcgcgt gcaatggtgc gcaactcgtc gggcgtcgca tcaatgcaag taagatgcgg
      781 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
      841 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
      901 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
      961 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
     1021 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
     1081 cacatctatc cgtttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
     1141 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
     1201 aagaaaaaga aaatccggta tgcaaagcaa accggactgc aaatattaac taaccatgta
     1261 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaatcc tatgataatt
     1321 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
     1381 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
     1441 gtcgcgcccc gcgccaggcc cacggatcac cagcgggttg tcgcgatacc agcggctttc
     1501 gatggcaaag acgttatcgc acggcagcag tgatgccagc ggatggtctt cacgcaccgc
     1561 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
     1621 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccgtt
     1681 ttcaaagaag tggtcgatgc tgccgccttc gcaatgggca ggcaccagcg attccacgcg
     1741 cacctgatcc ggttcgatgt cgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
     1801 cacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
     1861 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
     1921 cgtgccggag aagatcccac tgatcgacaa aatagtatcg ccgctgtcga tcagatcgcg
     1981 cacggtatgg ttgatcggca agcccgcacc gacggtggcg ttgtatagcc agtgacgccc
     2041 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
     2101 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcaagatact ggtcagccag
     2161 ctgctggctg gcggtaacgt ccagcaccac taaatcatca tacggatggg cgcgcatcca
     2221 caggaacaac gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
     2281 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
     2341 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
     2401 ccagcgggaa ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
     2461 gaagacggac tgatgcaggc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
     2521 caccaggctg atgccgtcat cggactgcca ggtaaactcg accggctggc ctttcagttg
     2581 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
     2641 cagcgccagc ccctgacgca ggcgcagttc gccaggtaat cccgcttcgt cgaggatttt
     2701 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc agcaactggc gatcgttatg
     2761 cacgccaacc gccagtgggc gcacctgggc acgtttcagg atttggtcga tctctttatg
     2821 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
     2881 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggagcc
     2941 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctaccgg atactggctg
     3001 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cttcatccag
     3061 acgcagcaat ggcagcagac aggcatcttt cactttacgc ggatcggcac tgtatacccc
     3121 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
     3181 ggaatagtcg gaaccgttac gccccagcag caccgtttca ccggcgttgt tacggctgat
     3241 aaacccggtc accaccagac gtttacccgg atgttgtacc agtagctgtt gcagcaacgg
     3301 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcgcg
     3361 ggcatcaagc caggccgctg gcagcccttg ttgattaagc accgcagaca tcaggcgcgc
     3421 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
     3481 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
     3541 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
     3601 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
     3661 agtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagtact ccgccataat
     3721 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
     3781 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
     3841 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
     3901 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
     3961 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
     4021 aacaacgaca gcccgcccag gaaacgacgc agcgtctgct catcgccatc cagttcaaaa
     4081 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
     4141 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
     4201 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
     4261 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
     4321 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
     4381 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
     4441 aatgccgggc ttaagaaggt gttatccacc acactcaccg ccccgacttc ccttgccaga
     4501 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
     4561 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
     4621 aacaacacgc gatagcaacc gcgtttcgcc aggctgtcga acaggcgata gctaccgccg
     4681 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
     4741 tgaatcgcgg acatgccggt attagtaagt actgcaccag caccgccttc cagttctgcc
     4801 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
     4861 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
     4921 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
     4981 atgtgatgaa gttccctggg cttggtcggt gaaatgtcag gcaccagagt aaacattgtg
     5041 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
     5101 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
     5161 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtgtggt
     5221 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
     5281 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
     5341 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
     5401 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
     5461 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
     5521 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
     5581 gaaaagagcg gcgcggagtg gaatcgcctg atgcgctacg cttatcaggc ctacgtcata
     5641 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
     5701 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacga
     5761 tgaaatacag gctactgatc ctcagtctgc tggtttctgt gcctgcattt gcctggcaac
     5821 cacaaaccgg cgacatcatc tttcagatct ctcgctcatc gcaaagtaaa gccatccaac
     5881 tggcgaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
     5941 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
     6001 gtgaaaaggg caaatacgtt gttcgccgcg ttgaaggcgg actcagtgtt gaacaacagc
     6061 aaaaactggc gcaaacggcg aaacgttatc tcggcaaacc gtatgacttc agcttctcat
     6121 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttatcag aatgcgctgg
     6181 gaatgcgcgt gggcgaacag cagaagttga aagagttcga cctgagcagc ccacaagttc
     6241 aggcaaagct caaagaacgt tacggcaaga atattccgct tgaggaaacg gtggtctcgc
     6301 cacaagccgt tttcgatgcg ccacaactca ccacggtcgc caaagaatgg ccgctgtttt
     6361 cgtggtaatg gatgataaaa aaaataattc ccgccgtagt ctgtttgttg ctttgtagtt
     6421 gcacgacctt tccgcaagct gtcgctccgg taaacggtga tgcctccaat tagttgaatt
     6481 aaggatataa tactcgcttt taaggttgtt tcatggtcac agttaacctt cattgtcccc
     6541 gttgccagtc cgttcaggtt taccgtcacg ggcagaaccc caaaagacat gatcgattcc
     6601 gcggccgtga ctgtcaccgg gtatatcagc tcagttactg ttacgagccc cgtaaaccgg
     6661 gagttaaaga ccaaatcgct gaaatggctt tcaacggtgc cggggttcgc tatactgccc
     6721 gtacgctgaa aatcggcatt aatacagtca tccgaacttt aaaaaactct cgccacgaag
     6781 aataacgtca tccccggtcg ctcatgctga tgtggcactc atctgcgaac ttagtcagca
     6841 atggagcttt gtcggcagta aagcgcgaca gcactggctc tggtacgtgt acaacacgaa
     6901 aactggtggt gttctggcct acacttttgg cccccggact gatgaaacct gtcgtgaatt
     6961 gctggcgctg cttacccttt taccatcggc atgttgacca ctgacgaatg gggtagttac
     7021 gctagagagt taccaaagga gaagcatctg actggtaaga tttttactca gcacattgag
     7081 cgtaacaatc tgaccctgag aacccgaatc aaacgtctgg cccgcaaaac catctgtttc
     7141 tcgagttctg tagaacttca tgaaaaagtc atcggtgctt ttatcgaaaa tatatgttct
     7201 actaattgga gtcatcaccc ttctgatgag ctttccttgg atgattacca gactgaatac
     7261 acagccaaag atttttacct cattctgctc ttttttctca aatgacatat at
//
LOCUS       DAZUCE010000235         7271 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZUCE010000235 DAZUCE010000000
VERSION     DAZUCE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.235"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..970
                     /locus_tag="ACHY03_004312"
     CDS             <1..970
                     /locus_tag="ACHY03_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3907477.1"
                     /translation="KLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADFES
                     KVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPT
                     GIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESL
                     VASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKE
                     VRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLD
                     LVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(967..2103)
                     /locus_tag="ACHY03_004313"
     CDS             complement(967..2103)
                     /locus_tag="ACHY03_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS3907478.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMVANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(2109..2432)
                     /locus_tag="ACHY03_004314"
     CDS             complement(2109..2432)
                     /locus_tag="ACHY03_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFS3907479.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            2615..2758
                     /locus_tag="ACHY03_004315"
     CDS             2615..2758
                     /locus_tag="ACHY03_004315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907480.1"
                     /translation="MNNFSKTSTMQIKRIKPSIIYRLLLIGLGTPMVIYGRVRPLTIE
                     TRD"
     gene            complement(2977..4515)
                     /gene="aldB"
                     /locus_tag="ACHY03_004316"
     CDS             complement(2977..4515)
                     /gene="aldB"
                     /locus_tag="ACHY03_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFS3907481.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4623..5918)
                     /locus_tag="ACHY03_004317"
     CDS             complement(4623..5918)
                     /locus_tag="ACHY03_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFS3907482.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(6048..7199)
                     /gene="yiaY"
                     /locus_tag="ACHY03_004318"
     CDS             complement(6048..7199)
                     /gene="yiaY"
                     /locus_tag="ACHY03_004318"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFS3907483.1"
                     /translation="MAASTFFIPSVNVIGADSLADAMNMMAEYGFRRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSIIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         1826 a         1898 c         1734 g         1813 t
ORIGIN      
        1 aaaattacaa cttaagtacc gggagttaaa aattatctcg gtaatcgctg ccagtgaaaa
       61 tatcagccat gccgcgactg tacttggcat cgcacaggcc aacgtcagca aataccttgc
      121 tgattttgaa tcaaaagtgg gtttaaaagt ctttgaccga accacccggc agctgatgct
      181 cacacctttt ggtaccgcgc tgttaccgta catcaacgac atgctggaca gaaacgagca
      241 gcttaataat tttattgcag attataagca tgagaaacgt ggtcgggtca ccatctacgc
      301 cccaaccggc ataattacct atttatccaa acatgtaatc gataaaatta aagatatcgg
      361 tgatatcacc ttatcgctaa aaacctgcaa tcttgagcgt aatgcctttt atgaaggcgt
      421 cgaatttcct gatgattgtg atgttttaat cagctacgct ccaccaaaag atgaatcgct
      481 ggttgccagt tttattacac aatatgccgt aaccgcttat gccagccagc gctatcttga
      541 gaaacatcct attagccgtc ctgacgaact cgaacatcac tcctgtattt tgatcgactc
      601 gatgatgatc gacgacgcga atatctggcg cttcaacgtc gccgggagta aagaggtgcg
      661 ggattatcgc gtgaagggaa attacgtttg cgataacacg caatcggcgc tggagctggc
      721 acgaaatcac ctggggattg tgtttgcgcc agacaaaagt gtgcagagcg atctgcaaga
      781 cggcacgctg gtgccctgct ttcagcaccc gtatgaatgg tggctggatc tggtggctat
      841 cttccgtaaa cgggaatacc agccctggcg ggtccagtat gtgctggatg aaatgctgcg
      901 tgaaattcgc caccagcttg ctcagtcgca gcaactgcgg cctgaacaag cggcggaaag
      961 cgaagattaa tgatcgagat aaaggtaatg cacccagctg gtcatgcgta ataacacttt
     1021 gcgcatcacc gagacatggc tgaaatgatc agagtaaacc gccacctggg cgtaaatacc
     1081 gtctggtaaa gcgctcaaat cagtgtgctc atccagttca atggtcgcga taacgccatc
     1141 tgaacccggc gctgtgttta acgtctgtaa ggtgccggtc gactgataag ctccgccggg
     1201 aacggctgga cttatggctg ccagcttacc gctgaatacc ttacctggca gagcattaaa
     1261 caccacttcc gcatcgtcgc caggagccag tcgcagcaag gagttctgac ggaactgcgc
     1321 cacgatttgt cgtttctgat cgggtataaa caccatcacc ggacgtagcg gcagcgacgc
     1381 ggcataggta cccggacgaa tcaggacctg agtcacataa ccatcgctcg gcgcacgcac
     1441 tatcgtctgc tcaaggttat attttgcttc cgcgagctgc gctttcaggc tggcgatttg
     1501 agaatgttca cccaacacca ggctatccag ctggctctgg atctgttttt gttccgctgc
     1561 cgatgacttt acggaggctt cctgcgccag ataattttgc cgcgccacat cgatatcgcg
     1621 ttctgaaaac gggtttactt tcgcctggct gccgcgtgcg taacgctgat actctttagc
     1681 gaatttatcc cgcgtggcct ttgcctgctg agtattcgcc accatctcat ctaactctgc
     1741 gcccaacgcc cgctgtttat gttctgcggt aacgatatcc gccatcagcc gatccacccg
     1801 cgcctgataa cgcgtcgggt ccagtcgaaa tagcacttca ccttttttaa tcagcgtatt
     1861 tttcttatcc gtcacttcaa tcaccacacc agtcacctgt gggacaacag gaatagaaat
     1921 aaccgctttt tgcgctttaa aggtatacgg atggttatag ttcattaaca gaattagacc
     1981 gctgacaata aatattccac ccagggccgc tgtgggaatg gtccatttat ttacgggaat
     2041 tttgaagatc ttaaacattg cccatgcaaa agccacatag gtcaaaataa tcagtaaatc
     2101 cataagtatt actcggcaga agaggatttg atgtcggcga gctgtttctc cagcccggca
     2161 atgcgctgtt gcagttgcat aacggatgaa tcatggcttt gcattcccca tccccgctcc
     2221 gggcggtaaa gcgtggccca aatccacaaa aatggccaga taacatgcag cgtaaaaaga
     2281 ctcacccaac cagcaacatg aatggcgtcg gcatgaggat gattacgttt tttagcaatc
     2341 aggtagggaa tatcatgtaa aataataatc ccatagaata tcaccagaaa tacaaaaata
     2401 agcactccca gtgcaaaata gtccaggaac atatttccct cagagaatat aaaaaagaac
     2461 aattaacgca tattatgaaa tgccatgatg caatgcaatt tccttattta tataacgaca
     2521 tattgttttt tatatacttt ctgaagtgtg ctgcgtggct ttttaacagg tcgagtctta
     2581 tcatattgaa ataatggaac tatcagccaa actaatgaat aacttcagta aaacatccac
     2641 aatgcagatt aagcgtatta aaccttcaat tatctaccgt ttattgctga ttggcctcgg
     2701 aacgccaatg gtgatttatg gccgggttcg cccgctcacc atcgaaacgc gagattaaac
     2761 tcctggcaaa acacgcccca gaaacgcact tctgttggcg tagtctgggt tattgcacac
     2821 gtaggtttaa aacgtaaatt gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta
     2881 cgccttatcc ggcctacggt caggtccccg taggcatgat aagacgcgta agcgtcgcat
     2941 caggcaatta atccccgatg cgaccagctt cttacatcag aacagcccca acggtttatc
     3001 cgagtagctc accagcaggc acttggtttg ctggtaatgc tcaagcatca tcttgtgggt
     3061 ttcgcgtcca atgcctgatt gtttgtagcc gccaaacgcc gcatgtgccg ggtaagcgtg
     3121 ataacagttg gtccacacgc gccctgcctg tatgccgcgc cccatcttat aggcaagatt
     3181 accgttgcgg ctccagacgc ccgcgcccag gccatattgc gtatcgttcg ccagctccag
     3241 cgcttcttcc atcgttttga aggtggtcac cgccagcacc gggccaaaaa tctcttcctg
     3301 gaacacccgc atattgttct gaccaaacag aatcgtcggt tcaaggtagt agccgtcttt
     3361 cagttcgcct tccagcagct tgcgccgccc gccggtgagc acgtctgcgc cctctttttt
     3421 accgatatca atgtagttga ggatggtttc cagttgcccg tgagaaacct gcgcacccat
     3481 ttgcgtcacg ctgtcgagcg ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg
     3541 ttccataaag cgttcgtaga ttgactcctg cactaaggca cgactcggac aggtgcaaac
     3601 ttcgccctgg ttaaaggcaa acagtgcaaa gccttccagc gctttatcga aaaaggcatc
     3661 ttcttcatcc atcacatcag caaagaagat atttggcgac ttaccgccca gctccagcgt
     3721 caccggaata atgttttgcg tggcgtattg cataatttgt tggcccactt ccgttgagcc
     3781 ggtaaacgcc actttggcga tgcgtttcga ggtcgccaga tattcgccaa tttccccacc
     3841 agcgccattg accacgttca ccacgcccgg cggcagtaaa tcaccgacga tttccattag
     3901 cagcagtaca gaaagcgggg taagacgtgc gggtttcagc accacacagt tgcccgccgc
     3961 cagcgcggga gccattttcc agctcgccat cagcagcggg aagttccacg ggataatctg
     4021 ccccaccacg cctaacggtt catggaaatg ataggccacg gtttcgctat caacttcact
     4081 gatcccacct tcctgcgccc gaatacacga ggcgaaatag cggaaatggt caatcgccag
     4141 cggtacatcc gcagcactgg tttcgcgaat gggtttgccg ttatcccagg tttcagctgt
     4201 cgctaacagc tcgaggtttt gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg
     4261 atcctgcacc gaggtgtgcg cccatttatc tttcactttg tgcgcagcat ccagcgccag
     4321 atcgatgtct cgtttgcccg aagacgccac ttcgcacagc agctgcccgg tcaccggcgt
     4381 caggttctgg taatactcac catcggcagg ggctacccat tcgccgccaa taaagttgtc
     4441 gtagcgggct tttaacttga tggggaaacc atactcgccg ggcttaatct gtgctgaagg
     4501 gggattattg gtcatgatcg tctccttgcg gtgtgaggta taacaagggt agacgtggct
     4561 ggcgaaatct tcgccagtcg gtaacagctt tacgacggct atcacgaatt tacgggcaag
     4621 ggttacagcg gataatgcgc gaagtacacg gcttgctggt cattaatttt cttctcttgc
     4681 gcaaaccctt ctttaacaat tgtccgtaaa tcagctcgag ccgtattatc agagacgccc
     4741 aggctcgcgc taatttcagc aactgtgtac tctttccccg gattggcgag catcacattc
     4801 aggagcgtta cctggcgcgg attcaggcgt ttaagcacac cagagctaaa cagcacacta
     4861 tcgagttttg cgcttcgttg tgtaatttca tcgatatgct gctgccagtt ttgcagagca
     4921 cgtttaatga cacccgcctg atattcaagg aaataagtca gatccatacc atctgattca
     4981 gtgtactggt agctggcggc atatttgacc ggtgcagcat gaagcaaacg gctgatggaa
     5041 atatatttaa agacatcgta gcccgatttc agcatatacc agtaaaacag cgcccggcta
     5101 gtgcgcccgt tgccatcccg aaacggatgt tcatgggcca gcataaaatg cagaatgcag
     5161 gcacggacca acggatgaat atacccttca tggctgttta accactggca aactttttcc
     5221 agccgttccg gtaacaatgc cgcagcgggc ggttgatgca ctatattgcc atcatagtcg
     5281 gcgatcacaa catcatctgt ttcacgaaat tctcccggac gatatttcgc gtcattgatt
     5341 ccacccatac caatggcatg taactggcga ataagcgcag gtgtcagcgg ttctgcgagt
     5401 aagtgcggaa tttccgccat cagccgggca tttcccgcaa tcatatgctc gtcttcagtg
     5461 cgtggcgacc gtcctgtttc cagcattttt ctcgccacca gcgttgtagt attcgcacct
     5521 tccagctggg agctggtgat cggctcttcc aggcgtaact ggcttaactc ggctccggcc
     5581 ccgcgtaacc gttcagtctg atctttcagc gccagcgcgg tagcacgttt gtcgacaagc
     5641 tcgcacgctt ctgcaatcac cggggtaatg ttgaatccgg cctgttcacc cgcttcattg
     5701 caataattaa ttcgttggat cgccgcgttg cgcgccagac gggtaagcgt ccacgccaca
     5761 tcgacgtttt ctcctttttt gatgcggtac tgcaactcat caaaaggcag ataacgtcct
     5821 ttttcatcgg taacccggtt aaagcgcagg tgggccagga gttcctcacc cgataagtgt
     5881 gataaaggaa taagtccggg aggtgatttg atagccatgt gcttatctca aaaaagcgca
     5941 atattgagat agtgtatagc aggaaagaga aactatctca aaaaaagcaa gttttgagat
     6001 agttgaaaaa gcggctgaca atttgccagc cgttgtggga atgatgatta cattgcggcg
     6061 cgataaatcg ccacaatttc ttcgtgagtt gcctggatcg ggttagtaaa gccacaggca
     6121 tctttcaggg cattagtcgc cagtaccgcg aaatcttctt ctttcacgtt caggtcgcgt
     6181 aggcctgccg ggatatccac tttcttcgcc agttcacgga tggcgttaat gcaggcttca
     6241 gcaccttccg cgtcgttttt acctgtcacg ttcacgccca ttgcagcggc acagtcacgc
     6301 agacgtgcag cggcgacttt gctgttgaat acctgaacat gcggcagcag aacggcgtta
     6361 catacaccgt gtggcaggtt gtagaaaccg cccagctggt gcgccatcgc atgaacataa
     6421 ccaagagaag cattattgaa cgccataccg gcaaggaact gggcataagc cattgcttca
     6481 cgcgctttcg cattactgcc atcttcaacg gctaacggca ggttttcggc aatcatggtc
     6541 acggctttca gtgcacaagc gtcagtgatc ggcgtggcgg caatagaaac atacgcttcg
     6601 atagcgtgcg ttaaggcatc catacccgtt gcggcggtca gtgacttcgg cataccaatc
     6661 atcagagagg agtcattgac agaaagcagc ggagtgacat gtttatcaac aatcgccatt
     6721 ttgatatgac gcgcttcgtc agtgatgatg cagaaacggg tcatttcaga ggccgtaccc
     6781 gccgtggtat tgatggcgat catcggcagc tgcggttttg cagagcggtc aacgccttca
     6841 taatcacgga tatcaccacc attggctgcc accagcgcaa taccttttgc acagtcatgc
     6901 ggagaaccac cgcctaagga gatcacgcta tcgcaattat tttctttaag taatttcaaa
     6961 cctgcggcga cgttttccgt ggttgggtta ggttgggtgc catcataaat aatgctaaaa
     7021 atattgcgtt cttccagtgc tttttgcaca tcgcccgcca tacctaattt cgttaacata
     7081 ctgtcagtga caatcaatgt acggcgaaac ccatattctg ccatcatatt cattgcatca
     7141 gccaatgaat cagcgccgat gacattcaca gaaggaataa aaaaggttga agctgccata
     7201 acacactcct gaaagtgtaa aaaagggcgg gcagcttact gaggattttc attcgaaaat
     7261 atgatcagta t
//
LOCUS       DAZUCE010000236         7249 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZUCE010000236 DAZUCE010000000
VERSION     DAZUCE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.236"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            193..495
                     /locus_tag="ACHY03_004319"
     CDS             193..495
                     /locus_tag="ACHY03_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907484.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPARRQVKSPVATKP
                     VQKKKSAAVRKVSQPLVAEKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            692..1893
                     /locus_tag="ACHY03_004320"
                     /pseudo
     CDS             692..1893
                     /locus_tag="ACHY03_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            2128..3873
                     /locus_tag="ACHY03_004321"
     CDS             2128..3873
                     /locus_tag="ACHY03_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS3907485.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            3939..4244
                     /locus_tag="ACHY03_004322"
     CDS             3939..4244
                     /locus_tag="ACHY03_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907486.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            4299..4613
                     /locus_tag="ACHY03_004323"
                     /pseudo
     CDS             4299..4613
                     /locus_tag="ACHY03_004323"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            4938..5087
                     /locus_tag="ACHY03_004324"
                     /pseudo
     CDS             4938..5087
                     /locus_tag="ACHY03_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5262..5696
                     /locus_tag="ACHY03_004325"
                     /pseudo
     CDS             5262..5696
                     /locus_tag="ACHY03_004325"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            5839..5928
                     /locus_tag="ACHY03_004326"
                     /pseudo
     CDS             5839..5928
                     /locus_tag="ACHY03_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6333..>7249
                     /locus_tag="ACHY03_004327"
     CDS             6333..>7249
                     /locus_tag="ACHY03_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HFS3907487.1"
                     /translation="MAKMIPAFGPIDNNSCGEKTVYTLLKEGLSDDFTVIHSLPWLSA
                     ATRNLGMKLPPSGEIDFLVLHPVYGVLALEVKSGIYRVEGTVFVHIKTKKHVDIVKQT
                     RNNVHGLARWLGGATTLRLRIGYGFIFPDSYFDDKIINAAMVDASVKPFRGIFVDKSQ
                     LPEMAKHTIDIMQYWKHALGNNELGDERVQLIIKSVCPEFDGTPDWGIRIIYDNKLWL
                     RLTHEQIKVIELLSEYQRVVVTGWPGTGKTLIGIEFARRLVKGQKKVLFITFNNLLSE
                     YIRQQTPESLCDVFTWHKLCHQARNKLSLS"
BASE COUNT         1963 a         1557 c         1911 g         1818 t
ORIGIN      
        1 tttttaggat tatcaggtgc gaaactcatc ggggcgcagg catccttgcc tacagagacg
       61 ccggatagat ttaagcaagc ccaccattaa ttaaaaaatc agtgttgcaa aaacgggggc
      121 gaccatagat tattttcggt tcagacagat gctgcgcggg gaagaaaacc ctgctcgggt
      181 gctaaaggtg gtatttcaca ctgcacgtca gacggacgtt ttgtctgcaa tgacggaagt
      241 ctgagtcagt cgaagcgctt ttgttccggt tatggcgtgg cacccgccag acgtcaggtg
      301 aaatcaccag tcgccactaa gccggttcag aagaaaaaat cagcggctgt ccggaaggtg
      361 tcacaaccgt tagtggctga gaaaaatgag gttgtaccgg tcactgaacc ccgtaaaccc
      421 acctgtgcgc cgctttatat ggccagcaaa cccggattta cgcatctgcc catttgttct
      481 ggtaatcagt attaatctgt cactgaaata gcagaggaat ctgtgctcag gctgcagttc
      541 atcctgtact tgcgggcatc ataaagtcgg acaaccttcc ggatataaat atttccggag
      601 agcgttaatc tgagatattc agttactgag aaggaaaata aaggagagtc atggcaggaa
      661 taaaaaagat aataatgcag cgggatttgt cctgatttta gtttgcgtta ttctctgggg
      721 gatttatgtg gcggtcagag cgctgataaa tcttaatgag cgctttattg atgcagtatc
      781 gaatcagaca ggtattattg gtttgttttt gggtttgctt actgtatttg caattttact
      841 tcgttttttt atttaccgga gactcagaaa gaaaacagcc gcctttgagc aggccgtttc
      901 agcgctggtt cagagagaac gggattttaa tgaaactgtc aatgccacga tagcccgggg
      961 aatacgacag gaaaaggagc aacttgccag acgccgtgag gaatttcata ccgccagaca
     1021 gaaggccagc cgtgcaatgc aacggattgt tgattccgcc tggaaattta aggcgaagac
     1081 tctgctggct ggtgtaacga ttaataactg gcaaagcaaa tatgaccagc ttcgtaagga
     1141 aagagaggct tacgctgctg tcagtgagaa aatagccttt cttaacctgg aagataattc
     1201 tgactgggag agtgtcaggc aacagtttct ggacaaagtg gcattgctgg aaaagcgcag
     1261 gaagagaagg aatatcaggc agagcttaag cgtcagatac gtgaagaaaa agagcgtcag
     1321 gatgaacttg accggcgcca gcgggaagcg gaagaggaag aacggcgtct tgctgagcag
     1381 cagaaactga ttgaggaggc gctgcgcgca gcagaagggg ctcacaggga ggagttagag
     1441 aaacaacgcc tggagctgga acagaaaatt caggaagcgc atgcgcagta tgaacgtgct
     1501 aaatccatgg ctcagcttac gaaacagggg catgtttata tcatttcgaa tattggttcg
     1561 tttggtgagg atgtctttaa aattggcatg acccgacggc tggaacccat ggacagggtc
     1621 aaagagttaa gtggtgcttc tgttccgttt gattttgatg ttcacgccat gatttcctgt
     1681 gatgatgcgc ctgcgctgga aaaaacactt catgacagtc tggaaaaata ccgcattaac
     1741 aggattaacc tgcgtaaaga atttttccgc gtgaagcttg agaaaatcat taatgaagtg
     1801 gagcgtcacc acgggcagat ggaatatgtg gctgacccgg cggcgctgca gtacctgcag
     1861 agtctggaat acgcagaaaa tgaagcagcc tgacagtcag tgttctgatt ttaccccggc
     1921 cggatataac agaagggata tctgcagata ccctgcagca tggttcacag tattctggtc
     1981 atgttaatga ttctgttttt tatttctgaa taaagcgaca cccacgatat aacatccgtg
     2041 gatgtcggtg gtgattattt tttgttcaca gattaaggtg cgtttatggc gaaactgata
     2101 aatgtatcgg cctccactga ggagagaatg acgcctgggg agaggagggt ggcttcacga
     2161 ctggagagct ttctgaatga tgactgcctt gtctggtacg atattccggt gggacggaaa
     2221 aaccggcatc cggattttgt cattattgac ccggagaacg gactggtatt tctggaggtc
     2281 aaggactgga cggtttccac gctgcgccag gtaaatcagg agcaggtcac gctggaaacc
     2341 gacggtcttc tgaaatcaga aatcaatccg ctggtacagg ttcgtcgtta tgcctgtgat
     2401 acggttaatg ccttacctgc aaacccgtgc ttacggcaga atgacggtca gtataaggga
     2461 cggctgaacc tggcctgggc gtatggtgtg gtgtttaccc gcattacccg tcagcaactg
     2521 aagacactgg cagggaatga tgagaatgcg gtggaaaaaa tttttccttc agcacagaca
     2581 atctgccagg atgaaatgac gcagtctgtt ttacctgagg tattccagca aaaaattgcc
     2641 gggatgttta ccacgggatt cagaacccgg gtgacacccc ggatgagaga tattctgcgc
     2701 gcccatctgt ttcctgaagt gaccgtgaaa cagaactcac aaatcaaaat catggatatt
     2761 cagcaggaga ttctggccag aaatattggt gacgggcatc gtgtcattca tggtgtggcc
     2821 ggttccggaa aaaccatgat tcttttgttc cgttgccttt acctggctga aaccacaccc
     2881 ggaaagattc tggtgttgtg ctacaacatt accctggcca gctatctgcg ggaatgcatt
     2941 gaagccagag ggttggcgtc acgggttacg gtatcccatt ttcacagctg gtgtgcctca
     3001 atggtaaaac gccacggtat taaggtgacg gccgacagaa aagaatatcc tgataaatgc
     3061 ttcagtgcac tggaagaggc cgtgaacagc ggaaaaatca cccgaaccgg atatgatgct
     3121 gtactggtgg atgaggggca tgattttgac agtcgctggc tggccctgat tgcccgtctg
     3181 tttgataacg catcccgttc actgctcctg atgtacgatg acgcccagtc catttaccgg
     3241 cgggagcggg ccctgaactt ttctctggcc agtgtgggga ttcaggcaca gggacgcact
     3301 tctgtcctgc cggtcaatta tcgtaatccg aaacgcattc tgcactttgc ttatgcattc
     3361 tcgcgcgagt attttgaaaa acatcagaac cgtgaaatac cgctcgttca gccacaggcg
     3421 ggcggtgaag agggtacgga gccggaaatc ctgcgttgtg cttctgaacg tgatgaagcc
     3481 agacaagttg cagactggct ggagaagcgc catgccctgt gtggtcactg gagtgatatg
     3541 gctgtgctgt gtccgacaga gcgttcagcg caacaattgc aggacgttat gacactacgc
     3601 ggggtgcctt ttgccacctg ttttgacagg gaaggcaaga agacatactc gcgacgtaag
     3661 gatgtggtgc atttactgac gtaccagagc agcaaggggc tggagttccc gtatgtggcg
     3721 gttatcaatg cgtctttcgt tcactctggt gcagcggatg agtctgaagt gattccggtg
     3781 ctttatgtgg cgttcacccg ggcaacccgt gaattactgg tgacctgtta ccgtgaaaac
     3841 agtatcagtc gtcaccttga ggattttgca taataccgga ttatttcttt attccggact
     3901 ttcatcatac tgttattatc aggtttattt aattcgccat gaacccgtta tttaatgata
     3961 ttcagatgcg tcttttttat ctgaaccact ctccgtactc ctggcactgg aatgtcagat
     4021 tcagacccca ggaagctgtc tatattggca gcgatacctg tcatatcacc attacctgta
     4081 atcagtccgg ttttcattta accagggacg gacaacggct gttcactgaa cgttatatca
     4141 gaaaccttaa tgagctgtta ccggttctga aacggcggtg ggatgtgaca ccggcaatca
     4201 tccgtgccgt ggaatacctt tcccgggtcc cggtattaca ctgacattct tttcgtcact
     4261 gttaatcatg ctttaataca gtagcaccga atctgccgat gacttctgaa gtgaaactga
     4321 aaaccggtgg tgaccccagg tcattaccgg attatgctgc cctgcgtgat gaaatcagca
     4381 aactgaccca cccggcacgt ccggatgtgg actggcgtta cgtggaaacg ctctgcctgc
     4441 gtctgtatga acacaatggc gtggaattac agacggcatc ctggtacacc atcgcccgga
     4501 tgcatacagc agggttaagt ggactgaatg aagggctggc tctgattgtg gcgctgaccc
     4561 ggcatcactg gtcagtgatg tggccgctga atacccacgc ccggctggaa attattaccg
     4621 gtctgttcaa ccgtctgcaa aaaacgcttc gggccatgcc tccggacgac cgtgacaacc
     4681 tgcccctgct gtatcagacc gaaacattcc tgaaggcact gagtgacaca cttgcccggc
     4741 acgaacttaa acagggcagt aaagtggctt taccggaagc catggtgaag ggatatatca
     4801 cccgcctgga aaatcagcct gtgcagggcg aagcgtcgtc tccggtaacg cttccggctc
     4861 aggcactccg gtctgatgtt ccggatgtgc aggaacatca gaccctgccg cactcccgtc
     4921 tggtgtatgt tgtttctgaa acaaaagcag agcccacacc ctcccttcag aaatccccgc
     4981 ccatgtttct gaagccgttt ttggctggtg tctgtgcggc attgctggct gtcagcgtgg
     5041 ccattccggg atggcagttt ctgacacagc catcaccggc ggagcagcaa ctcagggcgc
     5101 tgctgacacc cccaccggat gtgctttcgg cagaccagat agcacagctc agcagaacac
     5161 gctcactgtt aaatcatacg tcagagtgga tacccctttc cggtcaacag ataacgcgac
     5221 tgacagaatt acccctggca tacaacctgc agcgcagtgc gcagctgctg cagcaactga
     5281 gggtactttt ccccgataat ccccgggtac aggaaatggt ggataactgg cagaagagtg
     5341 taaggagccg tgcgctcccg gaagaggcga tgacgggctg gaacgaaggc atgacccgcc
     5401 tacagcagct ggctgagcgc ctgaaccgtc tggatgaaca gcggggaaaa tacatgacag
     5461 tcagtgaact gaaaacagag gtgtttggca tcatgcaggc ttttaaccgg catatcccgg
     5521 cagaagagca gttacgtcgc tataatgaag tccgcaacca gaatggcagt gaacaacagc
     5581 aaaaactgac gcaggatgcc ctgacggaac tgttgaaccg ctactggttt ctgagacatg
     5641 gagatgcagg taagaagacg ataaataata aacaggataa ccagattaat atctgacaga
     5701 ttttattgcc attatttatc cagaagcgat gatgccatta atgcaaaatg acatctgaat
     5761 tgatcacaga ttggaccaat ctgtgatcaa atagaacaga atacgcagat gataaagcgg
     5821 ctttccctag aagattcaca acgtcatctg gcagaatatg ggtgagagtt gcaggctgat
     5881 gcgtacaaag cttacgatgc gttgtatgaa actggccggg tgaaagatgt catccgtacg
     5941 gtgcgcgcca gactaaacag gcatcatgat ggaaaagtcg ggtatcattc acaggcatat
     6001 ttgtcagaat ggtagcgtca gcgggttttg attacaccag ttttgaagct catacttcct
     6061 atgaggcact gcagaaaacg aaagtgtcgt ttaggcgttg ggggaggggt taaaaaagta
     6121 acggcagaag tcaccctgaa gttgaggcag tcaactactg ttagctggct gctgcatgag
     6181 agtgtggagg gagattgtat atattggtta gtcagagtct gcttcggaat aagtatgagt
     6241 aatttttaaa atgggcggaa gttattttta attactgggc tatgtaacaa atgcagattc
     6301 ctgtcagaaa catgttataa ggaaatagta atatggccaa aatgattccg gcatttggac
     6361 ccattgataa taatagttgt ggggaaaaaa cagtctatac actgttaaaa gaaggtctgt
     6421 ccgatgattt cacagtgatt catagtctcc cgtggttaag tgcggcgaca cgaaatttag
     6481 ggatgaaatt acctccgagt ggagaaattg attttcttgt tctccatcct gtatatggtg
     6541 ttttggcgtt agaggtaaag agtggaatat atcgcgtaga aggtactgtt tttgtacata
     6601 taaaaacaaa aaaacatgta gatatagtga agcaaacacg taacaatgtc catggtttag
     6661 cccgttggtt agggggagca acaacgttac ggttgcgtat tggttatggt tttatttttc
     6721 ctgatagtta tttcgatgat aaaattataa atgcagcaat ggtagatgcg tctgttaaac
     6781 cattcagggg aatatttgtt gataaaagcc aactcccgga aatggcaaag catactattg
     6841 atataatgca atactggaaa catgcattgg gaaataatga gttgggggat gaacgggttc
     6901 aactaataat aaaatctgta tgtcctgagt ttgatggtac tcctgactgg ggaatccgta
     6961 ttatttatga taataaatta tggttaaggc tcacacatga acaaatcaag gtcattgaac
     7021 ttctgtcaga atatcaaaga gttgttgtaa ccgggtggcc aggaacagga aagaccctga
     7081 ttggaattga gtttgcacgg cggcttgtta aaggccaaaa aaaggtttta tttattactt
     7141 tcaataacct tctttcagaa tatattcggc aacaaactcc ggagtcatta tgcgacgttt
     7201 ttacctggca caaactttgt catcaggctc gaaacaaatt atccctgtc
//
LOCUS       DAZUCE010000237         7233 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZUCE010000237 DAZUCE010000000
VERSION     DAZUCE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.237"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..992)
                     /gene="pyrD"
                     /locus_tag="ACHY03_004328"
     CDS             complement(<1..992)
                     /gene="pyrD"
                     /locus_tag="ACHY03_004328"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFS3907488.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIK"
     gene            complement(1103..1840)
                     /locus_tag="ACHY03_004329"
     CDS             complement(1103..1840)
                     /locus_tag="ACHY03_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFS3907489.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEVSTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1806..2321)
                     /locus_tag="ACHY03_004330"
     CDS             complement(1806..2321)
                     /locus_tag="ACHY03_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3907490.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVLLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2329..2871)
                     /locus_tag="ACHY03_004331"
     CDS             complement(2329..2871)
                     /locus_tag="ACHY03_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3907491.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2883..3953)
                     /locus_tag="ACHY03_004332"
     CDS             complement(2883..3953)
                     /locus_tag="ACHY03_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3907492.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3944..6544)
                     /locus_tag="ACHY03_004333"
     CDS             complement(3944..6544)
                     /locus_tag="ACHY03_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFS3907493.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACALLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQERLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPINLLLPRSNDSYTSKKNYAWMTSNTSIDNEGHTTQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGVQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(6569..>7233)
                     /locus_tag="ACHY03_004334"
     CDS             complement(6569..>7233)
                     /locus_tag="ACHY03_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3907494.1"
                     /translation="LTAILLSCSSAWAAGKGGIGLAATRLVYSEGEEQISLGVRNTSP
                     DVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGAPLAKDRETLFFTS
                     VRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLRCTSSADMVTVSNP
                     TPYFITLTDLKIDGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRTINDYGAETPQLNC
                     KS"
BASE COUNT         1839 a         1855 c         1618 g         1921 t
ORIGIN      
        1 tctttaatca gcggcggacc tttaaaaata aaaccagaat aaatttgcac cagtgaggca
       61 cccgcagcaa tcttttcacg cgcagcgata accgagtcga tgccgccaac accgatgatc
      121 ggtaagcgac cgtttaattc cagtgacaag cggcgaataa tttcggtgct ttttaactga
      181 agcggacgac cacttaagcc accggtttga tcgcaatttt tcattccctg aaccagagaa
      241 cgatcgagtg tggtattggt tgcaataacg ccatcaatat tatggcgaac taaactatcg
      301 gcaacctgga tcaattcttc ttcagaaaga tccggcgcga tcttcactgc gatcggcaca
      361 tatttatggt gcatcgcttg caaatcattt tgcttatttt taatcgcggt taagagatca
      421 tccagcgctt caccatattg cagcgtgcgt aatcctgggg tattcggcga tgaaatattg
      481 atggcgatat atcccgcata ggcatagatt ttttccatac aaatcagata gtcatctttg
      541 ccctgctcca ctggcgtatc tttatttttg ccgatgttaa tacccaggac gccgtcataa
      601 tgggcctttt ttacgttctc tacgaggtta tcaacgccaa gattattaaa gcccatacgg
      661 ttgatcaaac cttcggcatc taccagacga aagagacgcg gcttgtcatt acctggctgt
      721 ggacgtggcg tgacggtacc gatctcgatc gatccaaatc ccatcgcgcc taacgcgtca
      781 atgcactccc cgtctttatc aagaccggct gccagaccaa gcggattttt aaacgtcagg
      841 cccatgcagt taacaggttt cgcaggcact ttctgccgca ccagtgcttc aaacggcgtt
      901 cctgtaatac ggcgtaattg ctgaaaagta aactcatgag cgcgctctgg atcgagctgg
      961 aaaagggctt tacgaacgaa ggggtagtac atgaactctc ctggattccc ggtgtgcaaa
     1021 ccgggggcgt attatgtgcg agttcggacc aaaagggaat tgacctgcgg caaaaaaaac
     1081 gcaaacgttt tccgaacctg tttcagggca gattttgctc cttctttgca ctcaacccgc
     1141 cgtaatcatt tatcgcccgc caggcgacat gacacggtgc gattgcctga caccagcttg
     1201 tctgacgctg gcttttaggc ggcacaactc ccgcattact taacgcttta ccatttacgc
     1261 tcacctgaat caggttaatg tagtaaggcg ttgggttagt gagttgtaca ccctgcgaat
     1321 tccgtgtcca gcgtaattgt tgataagctt ccagcgggtc gcccggcaaa ccttcgggtc
     1381 gccagaacag tttaaatacc gagcgcatcg ccacttttac gctctgattt tcaactttgc
     1441 cggatggcac gctggcaatg cttaactcaa atagcgtttc gcgatccaca ggcaagatgt
     1501 cgctttccgt tctcagcaag cgcaacgtgc ctgtagtacc gggcttcagg agaataagtg
     1561 gcggagcggc cagtaatagt gctggcactg tcgacacttc ccccccggcc caacgcgttg
     1621 ggcgattgat tttgctgtta ataagccagg attcctgact ggtattagtc agtaaaatag
     1681 atatcgattc tctgtctgcc ggaaagataa atcgcgttcc gccgaccacc actcctgcct
     1741 gcaattcacc cgccaccagc agcgataaaa cggcgaaaat caagagcgct aatctgttcc
     1801 acgtattagt cataatgaag tacaaatgtc gcgctggcat tcactttacc cgactgcaca
     1861 tcgctattga caggtcgcaa ttgcgcataa aaagttaata gcaccttacc gctatgtagg
     1921 ctgtagtctt tactcgcctg gttaagcgga atcaatatct tcgcatcatc cagaatcgca
     1981 attgccagcc cactggctgc atccgtacca ctttcaatgg acagcatttc gctctgcact
     2041 ccgtcggggg ttcctgaaaa ggtgacgtca accgtgctga catctgttcc gcaatcctgc
     2101 aaattaatga tgaaagattt ctccgggcca taattcccat gaaaaaaacg tgtcagcgag
     2161 acatcgccta actgcacatt gatttgcttg ctatcctgtg ccagcacgca tgtattacgg
     2221 cgaaacgatc cgctaatgtt gaccgtgcca tcatgggcaa atagctcaca gccccccgtt
     2281 aatcccagta agaacagaat ccagattatc cgtttcaaca tcactccttc actggtaaac
     2341 cagatcaaaa tagagaaccg ccgtcgcatt accgcccgtt gctcccgcct ttgtggactt
     2401 ataacgaagt tgatatttga gtgtgttatc cccggctttt aagggcgtaa gaggctggac
     2461 ctgattgagc gggatttctt gctgactttg cgcatcaaga atttgcacgg caatccccgt
     2521 tgccatacca ccgcttccgc ctgtgtcagt tagtttgagc agtgtcggcg caagggtgtc
     2581 agcctcgccg ctaaaaagga cgtccgcgcc agtaatacca gcggcacaac cggtgatatc
     2641 gatattcaaa tcagcagcag tgcttacggt attcgcagcc tgaaaatcac tgatattaaa
     2701 atcaccaata tgaatatttt gtaatgcact tttggtggcg acatcacagc cctgatcgac
     2761 cacattccca taaatatgca tactaacttg ttcaggccca gccatcccga catggatgtt
     2821 aagaatgcta aagaacaaga taacgcactg aaatatcgtt tttttcttca tacgttcacg
     2881 cgttaatcaa aatttattct caccgttgcc gtggcttcaa atcgcccggt ttctggtggt
     2941 acacctgtcg tgctgactgg atagacattc attcgcgcga tactgtctgc atcatcagga
     3001 atagagagcg gtaatgttcc accagcaggg gaaataatgt tgttctggct atcggtcacc
     3061 accacgccca cgccaggacg tgacgtctta atcgcctggg gatagctggg gtctgtggtt
     3121 gccgtcaaac tcaggttgac gttaactaac cccgcgtttc ccgtgttaca cctgactgga
     3181 attgcaagtt ctgccgttct ggccccaaga ggcttatgcc ctacaacacg aaagttagcc
     3241 gcaaatacgt cgcccagatc gacctcgatt gtttctccgg cattgacgga acatgagcca
     3301 agtgaattga tagtaccaga caggtaatac actaaaaccg gtgtacctgt agtcgtgcag
     3361 gaatcacctg cgggtatgtt gtagcattca aataatcgcg ccagcgccat aggaggaatg
     3421 acgaccgaac caacgaacgg ctgcaatatt ttcagggaaa agctaccgct attaccgcta
     3481 gccccctggc cacctaaacg ttgaccgccg ggatcgcaaa tatgccatcg tgtatctgct
     3541 cctgtgccca cgttttcgaa aggaaccgca gtaggagaac tgcctttaac ctgcaataaa
     3601 actttggcgg caagatagtc attaattttg taccagttgt cgcctaaata gactaacggt
     3661 gaatccgcgg cgaatgccca tcggccactt ctgacattgt ctttatcgca atcacaggaa
     3721 acataataat cattcgattc gttccactga taaaatttgt ctttaactac tccagccgta
     3781 ttttcttctt cctccagggt tttaacgcca aaattgacgc caatatacgg attcccctga
     3841 gtggtatgac actgcccgct ataagcagca gtttgagcgc accatagcat taagacggcg
     3901 gcaaaaaata gtcgtagtaa taacacgcat ttttctccaa agattatctg catatggcat
     3961 ccattcgcgt aatcgcagag ttaatttgtt gttcggtaag ctggtaactg atatgacaaa
     4021 tttgatcggg agcttcgccc cagcgtacag ttatcgctcc ctgagcgggc aaaccggaca
     4081 tatagagcga gccatcatca tcaataatgc cgctattagt ctgtacgccg tccagcgtcg
     4141 ctatcgcgcc aaaacgcagc ggtataccct gctttgatgt tttcattaat acccttgctc
     4201 ccgcatgggc attgaactcc gccagaacca acgccccttt ggtgggaacc acgttgacta
     4261 ctgcattttc aagatccacg ttacgtttta acgatgccgc atcaagtgcg attcgatttt
     4321 cccgataaga agtggcatag gggacaacgg tatatccgcg ccagtcagtt ttcagcccgg
     4381 tgctgttcgc cacacgagta ttctctgcgc ctggtgctgc aatcagcacg ttagtttccc
     4441 ccagcgattg ccccagggta atgccctgtg aatgggcaac taaactgcct gaaagagcgt
     4501 agttcagttg ttgttgactg ccgttatcgc tgtagttgta gcccactcgg gcatctgcaa
     4561 acgccccttt gtagtccatg ctggcgctac cattagccgt tttcccctcg ctgttatatc
     4621 cctgttgcac gctgtagctc aggttaccgt catcgagtag tgtctccgtt aaacccaggt
     4681 tttgtgtagt gtgcccttcg ttatcgatac tggtgttaga ggtcatccag gcgtaatttt
     4741 ttttgctggt atagctatca ttacttctgg ggagcaacag attgatcggc agggaaaaat
     4801 taagtgcaaa cacctgatca gcatcaggtt gaccacggga cttactgtaa ttccaggaaa
     4861 cccctagcga gagatcttta atttgcgtgt tgtagccaaa ctgtaatagc cgctcctgtt
     4921 gatcggtatg ccagtaagtt tgctggctac cacttaaata aaacgaaccg tactcgccta
     4981 attgctggga gatattgacc tgcagtttgc cacgtttggt gtaaaacaaa ttgtaataac
     5041 gcgaccacat cggcgtatct tcatcatcac cgtcattaaa ttcgtagcca tccatatgtt
     5101 tatacatggt gtcggaaagg gtgtagaaac ccgacgtcga atagcgatag cccatcaatt
     5161 gaaagttagt acccaattgg ttgagcgttt tggcatacag aaaacgatat gactgacctt
     5221 tatgttcgct ttggtctgcc agcgtactct tcgcctgggt cacatcgaac gaaattgctc
     5281 cgaaatcgcc gaggttaaat cccagaccaa acatggcggc acggtaatat tcagcatatt
     5341 gtccaccacc ataccaagtc gttccccacg gtccgcccca ctgcaacgtg gcctgggcaa
     5401 atttgctctc ctgctgttca ttactattgg ttctgtattt cgccagtgtc accgcgtatt
     5461 tgattcgccc ctgacgctgg agtaatggca cgctggaaaa gggtacgctg tagctattga
     5521 cgctgccgtc cgcttctttg atttcaacta acaaatcacc gctcgacgac gtggaataga
     5581 gatcactaat ttcaaaagca ccaggcgata cataagtttg gtaaatggtg taaccatttt
     5641 gcttaatcgt tatctgggca ttgcttttgg cgataccacg caccacaggc gcaaaccctt
     5701 tcaggctgtc tggcagcatg ttgtcatctg acgccagctg cacaccacga aagctgacac
     5761 tatcgaaaaa atcgcctgca gtataatcat cacctacagt cagttcgcct ttcagaggaa
     5821 taaccgcccg ttgcaaatag ctgctgagat tcttccattc tgcggtttgg ccactactac
     5881 gactccatgt tgaattgttg cgcaatctcc atgggcctaa attaacgcca ctgtttaaat
     5941 tcagaaaata actgtcgcca gaatcactgt ctgcgctgct atgaatactg ttagccccgc
     6001 taaatgaata tcccagtaat agcgcgttga tcccttcatc ccaacgttca ggggggacat
     6061 aaccacgagc attacttttg atggcaattt gcggtactga aatttccaga cggagtttat
     6121 tcacattaaa agttacgctg gcatccggaa taatctccgc caatagcgca cacgcttgtt
     6181 tgtccagttg tttaaaagcc ggaaatgcat cagtattcac gcccattgca tccaggcttt
     6241 cagtcgtcaa acaggcggca atccccttct ctggcgttat ctcaacaaaa ttgacgttgc
     6301 gcgtatctac aattgtctga ttcagaacaa tatcgacacg ataaacccct ggcgttattt
     6361 tttgtccttt ttcgaaacga gacaaatcgg ccaccattga gggatcatct ttgaaaaagg
     6421 ctggatcgaa ccaggtttca gcttgtgcat tgaacgctgc cgacacaaac accaccagcc
     6481 caccaataaa cgatggcacg ccaccagagc ttaacaggct gtgttgtcgg tgagttctat
     6541 acatctcgct ctcttaactg aagacgaatt acgatttaca gttgagttgc ggcgtttccg
     6601 ccccgtagtc attgatggtt cgatacgtta cgctgctatt tttggcaccc tttggcagag
     6661 agaattggta tttatcaaag ggggaaatca tttgattttt aactaccttt ccatctattt
     6721 tcaggtctgt cagggtaatg aaataaggtg ttgggttact gaccgtaacc atgtcagctg
     6781 acgaagtgca acgcagtttc gccggagcct cgcctaaggg atacgctaaa ccttttggtc
     6841 gccagaaaag tttgatgacg ctttgtgtgg caatcttcag ggtattgcct tcttcccgct
     6901 tcgttgttga agggactgcc cgtacgctag tgaagaaaag agtttctctg tctttcgcca
     6961 acggcgcacc aatgtacata atgcgtaaca gattctcatt agccggattc agcacaaata
     7021 gcggtggtgt aataataaag tctgctgatt ttttattatc tggggtcatc acccatgact
     7081 gaataagata aggaacatcc ggactggtgt tacgtacacc cagtgaaatt tgctcttcac
     7141 cttctgaata aacaagacgt gttgctgcaa gtccaatccc gcctttacct gccgcccatg
     7201 cggatgagca gctgagtaat atcgccgtta aac
//
LOCUS       DAZUCE010000238         7230 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZUCE010000238 DAZUCE010000000
VERSION     DAZUCE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.238"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..163
                     /locus_tag="ACHY03_004335"
     CDS             <1..163
                     /locus_tag="ACHY03_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907495.1"
                     /translation="TITLVILFCRRPHCRKVSVRQYYLKRVIFYPTTLQRTAFKKCLF
                     LMRDIIISS"
     gene            124..243
                     /locus_tag="ACHY03_004336"
     CDS             124..243
                     /locus_tag="ACHY03_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFS3907496.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            278..622
                     /locus_tag="ACHY03_004337"
                     /pseudo
     CDS             278..622
                     /locus_tag="ACHY03_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(994..1260)
                     /gene="minE"
                     /locus_tag="ACHY03_004338"
     CDS             complement(994..1260)
                     /gene="minE"
                     /locus_tag="ACHY03_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFS3907497.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1264..2076)
                     /gene="minD"
                     /locus_tag="ACHY03_004339"
     CDS             complement(1264..2076)
                     /gene="minD"
                     /locus_tag="ACHY03_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFS3907498.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2100..2795)
                     /gene="minC"
                     /locus_tag="ACHY03_004340"
     CDS             complement(2100..2795)
                     /gene="minC"
                     /locus_tag="ACHY03_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFS3907499.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3314..3682
                     /gene="ycgJ"
                     /locus_tag="ACHY03_004341"
     CDS             3314..3682
                     /gene="ycgJ"
                     /locus_tag="ACHY03_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFS3907500.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMVSGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3802..4203)
                     /gene="pliG"
                     /locus_tag="ACHY03_004342"
     CDS             complement(3802..4203)
                     /gene="pliG"
                     /locus_tag="ACHY03_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFS3907501.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4445..4738
                     /locus_tag="ACHY03_004343"
     CDS             4445..4738
                     /locus_tag="ACHY03_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFS3907502.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4810..5469
                     /gene="ycgM"
                     /locus_tag="ACHY03_004344"
     CDS             4810..5469
                     /gene="ycgM"
                     /locus_tag="ACHY03_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFS3907503.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5561..6007
                     /gene="ycgN"
                     /locus_tag="ACHY03_004345"
     CDS             5561..6007
                     /gene="ycgN"
                     /locus_tag="ACHY03_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFS3907504.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6213..7124)
                     /gene="hlyE"
                     /locus_tag="ACHY03_004346"
     CDS             complement(6213..7124)
                     /gene="hlyE"
                     /locus_tag="ACHY03_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFS3907505.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         1858 a         1602 c         1701 g         2069 t
ORIGIN      
        1 aacgattaca ttagtcattt tattttgccg acggcctcat tgtcgaaaag tcagcgtacg
       61 acagtattat ctgaaaagag tgatttttta tccaactaca cttcagcgca ctgcgtttaa
      121 aaaatgcctc tttcttatgc gggatatcat catttcatca tgatgtcttt ggtgagcggt
      181 gaacacaata cacctgcgct gtctcttcag gatgaatccc ctggtctggt gcctgcgggc
      241 tgatgttgca gccgagctaa ggtcacttag acgctactat catttatcca atggcatgga
      301 atcgaaatca gtcgataccc gcagtatata tcgtgaactg ggtgcaacgc tgagttacaa
      361 catgcgtctg gggaacggta tggaaattga gccgtggctg aaggcggctg tgcgcaaaga
      421 atttgtcgat gataaccggg tgaaggtgaa taatgacggt aatttcgtca atgatttgtc
      481 gggcagacgt ggaatatacc aggcaggtat taaagcctca ttcagcagta cgttaagcgg
      541 gtatctcggg gtggggtata gccatggtgc tggtgtggaa tccccgtgga atgcggtagc
      601 tggtgtgaac tggtcgttct gaccatcaac gattaaactg cacttcggca cagttttcgt
      661 ttacaggatg ttaaaagaga aaattctggg gtaaaaaaag cccgccagtt acggcgggaa
      721 acctcatcct atgggagaac aatgaataat gaaattgcgg ggttatcatc tcccagcata
      781 tccacactaa caataaggtt atttactcaa ccatgcataa atattttgtt ttgtgcgtgg
      841 gaacagcctt aaggtgtaaa gggggaggtg gaaatagcaa tgaggagtat cagcaagaat
      901 actcgccgct ttaccacaac gtcgatgaga gggatgaaaa actcaaggca gagatgactc
      961 tgccttgaag ataaatgcgc ttttacagcg ggcctatttc agctcttctg cttccggcaa
     1021 ggtcacgttc agctcaagaa tagaaatatc gccatctttt tgctcaagct gtacggttac
     1081 catctcagga tcaatttgta cgtatttaca aatgacctca agaatatctt tacgcaactg
     1141 cggcagataa tgcggttctg catcgctgcg acggcgttca gcaacaataa tctgcagccg
     1201 ttcttttgca atgttggctg tgtttttctt ccgcgagaga aagaaatcga gtaatgccat
     1261 aacttatcct ccgaacaagc gtttgaggaa gcctttcttc tcttcttcaa tgaagcggaa
     1321 aggacgttct tctcccaaca gacgttctac ggtatctgcg taggctttac ccgcatcggc
     1381 gttaatgtcg agaatgaccg gttcaccctg gttagaggcg cgcaatactg attgatcctc
     1441 tgggatcacg ccgacgagtt tgatgcgcag gatctccagc acatcttcca tgctcagcat
     1501 gtcacctctg cttacgcggc ctgggttata gcgcgttaac agcaggtgct ctttaatagg
     1561 ctcttcgcca ttttctgcgc ggcgtgattt cgacgccaga atgcctaaaa tacggtcaga
     1621 gtcgcgtact gaagagactt ccgggttggt ggtaataatg gcttcgtctg caaaatagag
     1681 tgccattaac gcaccggttt caatccctgc cggggagtca caaacgataa attcaaaatc
     1741 catcgctttc agatcatcaa gaactttggc gaccccttca cgggtgaggg catctttatc
     1801 gcgtgtttgc gatgccggca gaatatagag attttcagta cgcttatctt taattaacgc
     1861 ctgatttagc gttgcatcgc cctgaatgac gttgacgaaa tcgtaaacga cccggcgttc
     1921 acaacccata atcaggtcga gattacgcag gccgatatca aaatctatca cgacagtttt
     1981 ctttcccttc tgggccaaac cagtggcgat ggccgcgctg gaggttgtct taccaacacc
     2041 ccctttgccc gaagtaacaa caataatgcg tgccatagaa attccttgtt aaaaagggat
     2101 caatttaacg gttgaacggt caaagcgttt tcgactaact gcagtcgcgc cgctttgcca
     2161 taaaattctg ctgggatttg atcactcagc cagtattcac ctgcgatgga caccagttcc
     2221 gccatcaggt tcgtacaaaa tatttgcgtt tcccggtcac cacttgcccc tgccagcgca
     2281 cgaccgcgca tcatgccata gacatgaatg ttaccatcgg caattaattc ggccccagcg
     2341 ctaacgtggc ttgtaacaat cagatcacat tgtggagcat aaatacgctg accggaacgc
     2401 accggggtat ctattaaacg cgtttttgtg accggcgttg tattttgcgc tggagcctgc
     2461 ggtgcgggag ctggacgtgg cgctttttcc tttccttccg tcaggatagg cagccccatt
     2521 ttttcaattt cggctttaag ttgcgcatct ttgcagccac ttacgccaat aacccgcaaa
     2581 ccggttgccg aaaccgcctt atgcatcgct gaccagttta ccgggtcttc cagtgcactg
     2641 acgttgagta caacgggggc atgttttaaa aatgcggggg cctgagcgat tttgtcttcc
     2701 agcgcctgat ggataacctt aggttctgcc tcatgcagat gaaccacaga taaagtgaag
     2761 ctactgcctt taagctcgat tggcgtgttt gacatcctgg ccttactcaa ttagctatta
     2821 atcatcgcca gcgcgcgatg atgttccgaa gactataagg catgttatag tctggattat
     2881 attgaggcaa gtcaccctcc catttattca gagtaaaagt ctattctgtg ataaatggcg
     2941 ctgattcata gcttaaaaaa tacccttgtc aatcaaccca ttgccgtcgt acttttgatt
     3001 cttcttattt acgcttcctt tttccgcacc ctaatcgtga cagcatcttc agacctgttt
     3061 aaatatccag tattgaggca agtgtatttt ttcaccacgc caggctttct taactttcca
     3121 tttttgcagc atcattaaag aacagatacc cgttatctta atatccgcca cagaattgct
     3181 ctatcataga aaccgcagaa gacgtctgat acttctgtca ggtcatcgta actcgtccag
     3241 gtcagcggtt ttgattacgg attgatgatg aaatgagcat aagtgttgta cggttggatt
     3301 atcaggatgt gctatgaata tgatgcgtat tttttatatc ggattgtcag gtgtggggat
     3361 gatgttctca tcaatggtgt ctggaaatga cgctggcgga cttcaatctc cggcgtgcgg
     3421 cgttgtttgc gacccgtata tttgtgtgaa ctcagatggc atttccccag agttaacaag
     3481 gaaatatctc ggcgaaaaag ccgctgaaaa cttacaatca ttacaaggct acgatcccag
     3541 cgaatttaca ttcgctaacg gtgtattttg cgatgttaaa gaaaaattat gtcgtgatga
     3601 tcgttatttt ggtgtggatg gtaagcggag tggaaaaatc aatcaaacca ccacaaagat
     3661 gttatttatg tgtcgtgagt gatgccttgt ttacattatg ttctattcgg cattgtctgc
     3721 tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtt tatgacgggg
     3781 agagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt ttttagtttt
     3841 gtttttacgg gcgtcgtttc gggtttgcag cactcgcagc tcatatttgc cagaagcagg
     3901 gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat caacagaatc
     3961 atcgattcct ggcccgaaga ggtaagtatc agcgccttca ttagagatac tcacatgcac
     4021 tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct ttatttcgcc
     4081 ggagtactga gcgctgctat ggccttttct gaactcaaca ttgacattct tacccgcagc
     4141 aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc tcttgatttt
     4201 cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa caatccctta
     4261 cgggagaatt ccagtggctt tcctgcgttc ggtaaaatcc cgtcgcggca tttttgccgg
     4321 ggggattact tataaagggg gcatggactc aacaaaccgt agaaatctac agctggctgg
     4381 cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct taaagagtaa
     4441 aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt tatatgtcga
     4501 aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg gtcagcctca
     4561 attagcgatg attctgccgc tggatgggcg gaagaaactg gtcaatgccg atattgaaaa
     4621 ggttaaacag gcattaaccg agcaaggtta ctatttgcag ttaccgccac cacccgaaga
     4681 tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta acaaataacc
     4741 gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg ataaggtgag
     4801 gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta tccggtgagt
     4861 aaagtagttt gtgttggcag taactatgcc aaacatatta aagagatggg cagcgcagtg
     4921 cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct gcggcagcca
     4981 ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc ggtgttgatt
     5041 ggcgcgacgc tgcgtcaggc tacggaagag catgtccgca aagccattgc cggttacggc
     5101 gtggcgctcg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccc
     5161 tgggaaaagg ccaaagcgtt tgataactct tgtccgcttt ccgggtttat tcccgcggcg
     5221 gaattcaccg gcgacccgca aaatacaacg ctgggcctga gcgtaaacgg cgagcaacgc
     5281 cagcaaggta cgactgcgga catgatccat aaaatcgttc cgctgatcgc ttatatgagc
     5341 aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga tggcgtcggc
     5401 ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt gacaactcgc
     5461 gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc tgtgccagac
     5521 tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg tacctttctg
     5581 gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg
     5641 cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct acttcactaa
     5701 cgtcgcctgt cgccagctca atattaaaac ctgtcaatgt cggaactacg aacgtcgttt
     5761 cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct
     5821 gccaatgacc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg cgtggcatcc
     5881 gctgcttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc
     5941 agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac ctgactgggc
     6001 gcagtgataa atttaataga tgttgatggg gctttcttaa tatacgtctg gatgctcgcc
     6061 atttcgtagc cctgtttttg aaagcgctct gtcataatga gagttacccg ataccttatt
     6121 atcaacaggt aactctcaat ctttaaaaaa cgtctgtatt tttaaatgtg ataaacttta
     6181 taccttgagt acatggagag aataatcgct tatcagactt caggtacctc aaagagtgtc
     6241 tttttaccgt gtcttttctg atactcatta caggtgttaa tcattttttt ggccgcttct
     6301 tttagtaagg aaagcattaa atcatcataa tcaacgtaga atctggtggt ttcagtttcc
     6361 gtttttatct ccccgatggc ggctatttcg gtggttaatt tcaatttggc ggcatcgata
     6421 tctttattcg cttgtttaac cgtgttagac agggtggtaa agaaactctg cacagatttt
     6481 aacttgttct tcaattctgg aatcagtttc ccctcaacta cgcccgcagc aatagaatag
     6541 gaaatgatta atccaaatgg accggcgacg acaccggctg cggcaccggc atacgcttcc
     6601 ttcctgattt tatctacctg tgactggaaa tagctgcttt tttctgaaaa atcattggtt
     6661 aactggctat ctaacgccag cagtttcccg gaagcgttgt tgaaactttg tgagcttacc
     6721 agcagggatt tttgcgcttc attcagcttc gtgatgccgt catccagtac cttaatgaga
     6781 atgtctttct gggcggatgc tttcttctca ttgtactcat caaatagcaa aatatacgct
     6841 gcgagcaatt gcgtcgcaac accacaccat tcatacactg tttgggttgc ttcaaaatac
     6901 ttatcctggc tatccataag taaggtttta atatcgccaa ctaaaacgga ggctgcctgt
     6961 gaatactcct gtttaaagcg acttaactct tttatggttt catcaaaggt ctgccagggg
     7021 atgacctgat cgagatattt attataaaga tccaatgctc catctgcggt ttcgattgcg
     7081 tttttaacta cctctaccgt tttatctgca acgatttcag tcataatcat tcgcctcttt
     7141 aaatatataa attgtaatga aactcctgtt ttacaactat taatgaattt tacttcatct
     7201 aattcatagt tagccgggag agatgcgtca
//
LOCUS       DAZUCE010000239         7204 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZUCE010000239 DAZUCE010000000
VERSION     DAZUCE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.239"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            333..1370
                     /gene="dusA"
                     /locus_tag="ACHY03_004347"
     CDS             333..1370
                     /gene="dusA"
                     /locus_tag="ACHY03_004347"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFS3907506.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCNFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1504..1746
                     /gene="pspG"
                     /locus_tag="ACHY03_004348"
     CDS             1504..1746
                     /gene="pspG"
                     /locus_tag="ACHY03_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFS3907507.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLTIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1912..2895)
                     /locus_tag="ACHY03_004349"
     CDS             complement(1912..2895)
                     /locus_tag="ACHY03_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFS3907508.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            2978..4393
                     /gene="dnaB"
                     /locus_tag="ACHY03_004350"
     CDS             2978..4393
                     /gene="dnaB"
                     /locus_tag="ACHY03_004350"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS3907509.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4446..5525
                     /gene="alr"
                     /locus_tag="ACHY03_004351"
     CDS             4446..5525
                     /gene="alr"
                     /locus_tag="ACHY03_004351"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFS3907510.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKVGEPVGYGGTWISERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5778..6971
                     /gene="tyrB"
                     /locus_tag="ACHY03_004352"
     CDS             5778..6971
                     /gene="tyrB"
                     /locus_tag="ACHY03_004352"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFS3907511.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTVNVQRVAKA
                     FAAVM"
BASE COUNT         1694 a         1771 c         1898 g         1841 t
ORIGIN      
        1 gtattacata aaagaaccca atgcttattg ggttcttttt tctctatcag tgctattagc
       61 agggagagat atcaccggag tttaatgtgt gatttttatt tatcgtcgaa tctgtattgt
      121 ttatcattgc ttttcacaac gctaacatct aataagatta gatcttcact tcgtgagagc
      181 ttcatgcctt gagaggatct catttttctt ttgcaatgag acaggcgctt cctgttgtta
      241 tggtatagta ccccgttatt gagcctcctg aacagtgatg ctgaataaca taactccatg
      301 atatatcgat aaaataatct ctacatttga aaatgcacgg taattctgaa atgcaaaaaa
      361 tcaaccaaac cagcgcaatg cctgaaaaaa ctgacgttca ctggagtggt cggtttagcg
      421 ttgcaccaat gctcgactgg acggacagac attgccgcta tttcttgcgt ctgctttccc
      481 gcaatacgct gctgtatacc gaaatggtga ccacaggggc gattattcac ggtaaaggtg
      541 attacctggc gtacagtgaa gaagaacatc cggtagcgtt gcaactcggc ggtagcgatc
      601 cggcggcgct ggcgcagtgt gcgaagctgg cagaagcgcg cggatatgat gagatcaacc
      661 tgaatgtcgg ctgcccgtct gaccgggtgc agaacggcat gtttggtgcg tgtctgatgg
      721 gtaatgcgca gctggttgcc gactgcgtga aagcgatgcg cgatgtggtg tcgattccgg
      781 tgacggtgaa aacgcgtatt ggcatcgatg accaggacag ctatgaattt ctctgcaatt
      841 tcatcaacac cgtttccggc aaaggcgagt gtgagatgtt catcatccac gcacgtaaag
      901 cctggctttc ggggttaagc ccgaaagaaa accgtgaaat cccgccgctc gattatccgc
      961 gtgtgtatca actgaagcgt gactttccgc atctgacgat gtcgattaac ggtggcatca
     1021 agtcgctgga agaagctaaa gcgcatttgc aacatatgga tggcgtgatg gtcgggcgcg
     1081 aggcgtatca gaatccgggt attctggcgg cggtagaccg ggagatcttt ggttcctcgg
     1141 ataccgatgc cgatccggtg gcggtagtgc gcgccatgta tccgtacatt gagcgtgaac
     1201 tcagccaggg gacgtatctc ggccatatta cccggcatat gctgggcttg ttccagggta
     1261 ttcctggcgc gcggcagtgg cggcgttatt taagtgaaaa tgcccataaa gcgggtgctg
     1321 acattaacgt gctggaacac gcgctcaaac tggtggcgga taagcgttaa cttttcacca
     1381 aaaagtagtc aaattcacca cgccctgcgc accgtcgcgg ggcgttttgc tgttaaatca
     1441 atagattatt tttggcatga ttcttgtaat gcctgcaaga gatttcatat ttgggagagc
     1501 atcatgctgg aactactttt tgtgattggc ttttttgtca tgttgatggt caccggcgtt
     1561 tcgttgctgg gcattatcgc cgcgctggtt gtggcgacgg ccattatgtt cctcggcggt
     1621 atgctggcat tgatgattaa gttgctgccg tggttactcc tgacgattgc ggtggtgtgg
     1681 gttattaagg cgattaaagc accaaaagtg ccgaaatatc agcgttatga ccgctggcgt
     1741 tactaaggga ttgtgcggat gatcacaacc taaggtttta tccttagaac aaaataggaa
     1801 ttgataatca aatctgtcac tattgcgcct ctaacagatt catcgtgctg taccctacat
     1861 acagccgaac tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat
     1921 caacaggctg gaaccttgcg tcgcgcggct ttccagaatc tcatgcgcac gctgcgcatc
     1981 cttcagcgga tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa
     2041 agagaacagt tcattactgg cctcggttag ttcctcccgc gtggtgatat agccttgcag
     2101 ggaagggcgt gtcacataca acgagccttt ctgattgaga atgcctaagt tcacaccggt
     2161 aaccgcacct gatgagttgc caaaactcac cattaagccg cggcgttgca ggcaatccag
     2221 cgaccgttcc caggtgtctc tgcccacgga atcgtacacc acgcgcactt ttttaccacc
     2281 ggtgatctct tttaaccgct cgaccagatt ctcttcacga tagttaataa cctgccacgc
     2341 gcccgctttt agcgcgctct gcgctttttg cgcggttcct acggtgccga taagtttcgc
     2401 gcccagggct tttgcccact ggcaggcaat caagccaacg ccgccagctg ctgcgtggaa
     2461 caggaactgt tcatcgggtt taatttcata ggttttgcgc agcagataat aaaccgttaa
     2521 gcctttcagg aaagatgctg cagcttgctc aaaagaaatt gccgcaggca gaatcgccgc
     2581 tttatccgca ttaatgttat gcacagagct gtaagcgcct aacgccgact gcgcatagac
     2641 tacacgatcg cctgccttaa tatgctttac accactgccg accttactca cgatgcctgc
     2701 cgcttcggta cctaatccgc tgggtagcga tggcggcggg taaaggccgc tgcggatata
     2761 cgtgtcgata aaattgatgc cgatggcttt attttcgacc tggatttcat tctccgccgg
     2821 atcggcagga gtgaactcta cggcttgaag tacttccggg ccaccgtgct tgtgaaattc
     2881 aattcgtgtt gccatgtgtc ctccagaacg tatcgtcagg gtctgcttca tatggtaaag
     2941 tttcgaccca ttctttatct cggtaactcc attcactatg gcaggaaata aacccttcaa
     3001 caaacagcag gctgaacccc gcgaacgcga tccacaagtt gccgggctga aagtgcctcc
     3061 gcactcgatc gaagcggagc agtcggtgtt gggcggttta atgctggata acgaacgctg
     3121 ggatgatgta gccgagcgtg tggtagcaga cgatttttac acccgcccac accgtcatat
     3181 ctttactgaa atggcgcgtt tgcaggaaag cggtagtcct atcgatctga ttactcttgc
     3241 ggaatcgctg gaacgccagg ggcaactcga tagcgtcggt ggttttgctt atctggcaga
     3301 gctgtcaaaa aatacgccaa gtgcagcgaa catcagtgct tatgctgaca tcgtgcgtga
     3361 acgtgccgtt gtccgtgaga tgatctcggt tgcgaatgag attgccgaag ccggttttga
     3421 tccgcagggg cgcaccagtg aagatctgct ggacctggct gaatcccgcg tctttaaaat
     3481 tgccgaaagt cgtgcaaaca aagacgaagg gccgaagaac atcgccgatg tgctcgacgc
     3541 aaccgtggcg cgtattgagc agttgtttca gcagccacac gatggcgtta ccggggtaaa
     3601 caccggttat gacgatctca acaaaaaaac cgctggcttg cagccgtcgg atttgatcat
     3661 cgtcgccgcg cgtccgtcga tgggtaaaac aacatttgcg atgaacctcg tcgaaaacgc
     3721 ggcgatgttg caggataaac cggtacttat cttctcgctg gagatgccat cagaacagat
     3781 catgatgcgt tctctggcgt cgctgtcgcg cgttgaccag actaaaatcc gtaccgggca
     3841 gctcgatgat gaagactggg cgcgcatttc cggcaccatg ggtattttgc tcgaaaaacg
     3901 caatatctat atcgatgact cctccggctt gactccaacg gaagtgcgtt cccgcgcacg
     3961 ccgtattgcc cgtgaacacg gcggcatcgg gcttatcatg atcgactacc tgcaactgat
     4021 gcgcgtaccg gcgctttccg ataaccgtac gctggaaatt gcagaaatct cccgctcgct
     4081 gaaagcactg gcgaaagaac tgaacgtgcc ggtggtggcg ctgtcccagt tgaaccgttc
     4141 tctggaacaa cgtgccgaca aacgcccggt caactccgac ctgcgtgaat ctggctctat
     4201 cgagcaggat gcggacttga tcatgtttat ctatcgtgat gaggtgtatc acgaaaacag
     4261 tgatttaaaa ggcatcgcgg aaattattat cggtaaacaa cgtaacggcc caatcgggac
     4321 ggtacgcctg acctttaacg gtcaatggtc gcgcttcgac aactatgcgg ggccgcagta
     4381 cgacgacgaa taataattat tttatgaatt aggtaattaa agcaaacact tatcaaggaa
     4441 cacaaatgca agcggcaact gttgtgatta accgccgcgc tctgcgacac aacctgcaac
     4501 gtcttcgtga actggcacct gccagtaaaa tggttgcggt ggtgaaagcg aacgcttatg
     4561 gtcacggtct tcttgagacc gcgcgaacgc tccccgatgc tgacgccttt ggcgtagccc
     4621 gtctcgaaga agctctgcga ctgcgtgcgg ggggaatcac caaacctgta ctgttactcg
     4681 aaggcttttt tgatgccaga gatctgccga cgatttctgc gcaacatttt cataccgccg
     4741 tgcataacga agaacagctg gctgcgctgg aagaggctag cctggacgag ccggttaccg
     4801 tctggatgaa actcgatacc ggtatgcacc gtctgggcgt aaggccggaa caggctgagg
     4861 cgttttatca tcgcctgacc cagtgcaaaa acgttcgtca gccggtgaat atcgtcagcc
     4921 attttgcgcg cgcggatgaa ccaaaatgtg gcgcaaccga gaaacaactc gctatcttta
     4981 ataccttttg cgaaggcaaa cctggtcaac gttccattgc cgcatcgggt ggcattctac
     5041 tctggccaca gtcgcatttt gactgggtgc gcccgggcat cattctttat ggcgtctcgc
     5101 cgctggaaga tcgctccacc ggtgccgatt ttggctgtca gccagtgatg tcactaacct
     5161 ccagcctgat tgccgtgcgt gagcataaag tcggagagcc tgtcggttat ggtggaacct
     5221 ggataagcga acgtgatact cgtcttggtg tagttgcgat gggctatggc gatggttatc
     5281 cgcgcgccgc gccgtctggt acgccagtgc tggtgaacgg tcgcgaagtg ccgattgtcg
     5341 ggcgagtcgc gatggatatg atctgcgtag acttaggtcc acaggcacag gataaagccg
     5401 gggacccggt cattttgtgg ggtgaaggtt tgcccgtaga acgtatcgct gaaatgacga
     5461 aagtaagcgc ttacgaactt attacgcgcc tgacttcaag ggtcgcgatg aaatacgtgg
     5521 attaatcgtt ctgtaatatt tgattgtctg tgccggatgc ggcgtgaatg ccttatccgg
     5581 ccaataaaat cctaccaatt caataagttg atattctttc atgctcttat aaaggtcgtg
     5641 cctctggcgg atgtacgttt gtcatgagtc tcactctgtt gctaattgcc gttcgcccct
     5701 gaacatccac tcgatcttcg ccttcttccg gtttattgtg ttttaaccac ctgcccgtaa
     5761 acctggagaa ccatcgcgtg tttcaaaaag ttgacgccta cgctggcgac ccgattctta
     5821 cgcttatgga gcgttttaaa gaagaccctc gcagcgacaa agtgaattta agtatcggtc
     5881 tgtactacaa cgaagacgga attattccac aactgaaagc cgtggcggag gcggaagcgc
     5941 gcctgaatgc ggtgcctcat ggcgcttcgc tttatttacc gatggaaggg cttaacagct
     6001 atcgccatgc cattgcgccg ctgctgtttg gtgccgacca tccggtactg caacaacagc
     6061 gcgtagcaac cattcaaacc cttggcggct caggggcatt gaaagtgggc gcggatttcc
     6121 tgaaacgcta ctttccggaa tcaggcgtct gggtcagcga tcctacctgg gaaaaccacg
     6181 tagcaatatt cgccggggct ggattcgaag taagtactta cccctggtat gacgaagcga
     6241 ctaacggcgt gcgctttaat gacctgttgg cgacgctgaa aacattacct gcccgcagta
     6301 ttgtgttgct gcatccatgt tgccacaacc caacgggtgc cgatctcact aatgaccagt
     6361 gggatgcggt gattgaaatt ctcaaagccc gcgagcttat cccattcctt gatattgcct
     6421 atcaaggatt tggtgccggt atggaagagg atgcctacgc cattcgcgcc attgccagcg
     6481 ctggattacc cgctctggtg agcaattcgt tctcgaaaat tttctccctt tacggcgagc
     6541 gcgttggcgg actttctgtt ctgtgtgaag atgccgaagc cgcaggccgc gtactggggc
     6601 aattgaaagc aacagttcgc cgcaactact ccagcccgcc gaattttggt gcgcaggtgg
     6661 tggctgcagt gctgaatgac gaggcattga aagccagctg gctggcggaa gtagaagaga
     6721 tgcgtactcg cattctggca atgcgtcagg aactggtgaa ggtattaagc acagagatgc
     6781 cagaacgcaa tttcgattat ctgcttaatc agcgcggcat gttcagttat accggtttaa
     6841 gtgccgctca ggttgaccga ctacgtgaag aatttggtgt ctatctcatc gccagcggtc
     6901 gcatgtgtgt cgccgggtta aatacggtaa atgtacaacg tgtggcaaag gcgtttgctg
     6961 cggtgatgta atgcaggaaa gcaggctgga attacccagc ctgcagtgaa attaaactgt
     7021 cgtcgctttc actctttctt tatagatgat ttttttgata ccatcgttct acgtgagaga
     7081 taataaacgt tgttagttct tttattgtta atattatcct aattatctgg aattcctttc
     7141 cctgtttttt gtgcggggta ataatttctg catttctaat cattattaaa cttaataata
     7201 agtg
//
LOCUS       DAZUCE010000240         7168 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZUCE010000240 DAZUCE010000000
VERSION     DAZUCE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.240"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            49..291
                     /gene="yiiF"
                     /locus_tag="ACHY03_004353"
     CDS             49..291
                     /gene="yiiF"
                     /locus_tag="ACHY03_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFS3907512.1"
                     /translation="MNSLAGIDMGRIILDLSNEVIKQLDDLEVQRNLPRTELLREAVD
                     QYLVNQSQTVRTSAFGIWQGREEDGVEYQRKLREEW"
     gene            complement(621..1550)
                     /gene="fdhE"
                     /locus_tag="ACHY03_004354"
     CDS             complement(621..1550)
                     /gene="fdhE"
                     /locus_tag="ACHY03_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFS3907513.1"
                     /translation="MSIRIIPQDELGSGEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1547..2182)
                     /gene="fdoI"
                     /locus_tag="ACHY03_004355"
     CDS             complement(1547..2182)
                     /gene="fdoI"
                     /locus_tag="ACHY03_004355"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFS3907514.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2179..3081)
                     /gene="fdoH"
                     /locus_tag="ACHY03_004356"
     CDS             complement(2179..3081)
                     /gene="fdoH"
                     /locus_tag="ACHY03_004356"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFS3907515.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3094..6144)
                     /gene="fdnG"
                     /locus_tag="ACHY03_004357"
     CDS             complement(3094..6144)
                     /gene="fdnG"
                     /locus_tag="ACHY03_004357"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5557..5559),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFS3907516.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6338..>7168
                     /gene="fdhD"
                     /locus_tag="ACHY03_004358"
     CDS             6338..>7168
                     /gene="fdhD"
                     /locus_tag="ACHY03_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFS3907517.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1590 a         1909 c         1938 g         1731 t
ORIGIN      
        1 agggtacttt tctctccata gatatcaaat gatattggtc aaatatttat gaactctctg
       61 gcaggtattg atatgggcag aattatactc gatttatcga atgaggtgat taagcaactt
      121 gatgatcttg aggtgcagcg taatcttcct cgtacggagc tattacggga agcggtagat
      181 caatacttgg taaatcaatc gcaaacagta agaaccagtg cttttggcat atggcaaggg
      241 cgtgaggaag atggtgtcga ataccagcgt aagctgcgcg aggaatggta atgggaaaaa
      301 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
      361 ccttatccgg cctacaaagt cgtataaatt caatctattg cagtttattg gtaggcctga
      421 taagcgcggc gcatcaggca atcttacgct tatcatcagt ctcataccca taaccttatt
      481 gatgtggggg tgggtaaatt gaggcaaaac aggcactggc ggcatagctt gaggtggtaa
      541 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgaccagcgc
      601 cacccggaaa acgtcagaga ttactcccct tcacccggaa acaggaacgg gttaatggaa
      661 ctgcgggcat agccttcttg ctccattcgt gcgtccagta ccagagaggc gaggtcatct
      721 gccacggctt caactttcgg ttctttttcc tgatagagaa ttttcaggta agtgccgcag
      781 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
      841 ttgccgctct gctcacagtt gctgcatttt acgcgcacta cgtgccattc agtttcacac
      901 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
      961 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     1021 ccggggatca gattggccat ctgcgcccag tagagcgaca gtgcagccca gataaacggc
     1081 gctttatcgc tactgacgga tgagaaatca gaggcaaaca gtgcgctggc catatcttcc
     1141 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     1201 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     1261 agaacgtgaa tatccagcgg gggcttgcct tgtgcgctgg cttctttaat gcgcgcagtc
     1321 agatccatct ccagcggatg gtcgtacagc accacttcct gggcgtgggc gataagcgca
     1381 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     1441 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     1501 cgtttctcgc ccgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     1561 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     1621 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     1681 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     1741 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     1801 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     1861 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     1921 aaaagatatc atcccgattg attaggttgt gatgccagta acggaaaaac atgatgatga
     1981 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     2041 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     2101 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     2161 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     2221 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ctgcgaaggt
     2281 agccgcaaag ccaacagctg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     2341 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     2401 cacgtacata acgtgtgtac caccgacgcc tgccggatcg tacaggcccg cattgtcgta
     2461 accgcgggtt ttcagctccg ccacgcgctc gctcgccagc gttttcatcg actctttcgt
     2521 accgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     2581 cacgcggtca acgcacaacg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     2641 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     2701 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcagaca
     2761 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     2821 ctgctccact tccgagaagc gcatcaccgt ccacgatttg gcacttaaat cattggggtt
     2881 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     2941 cacctgacag gctttacagc cgatacaggt ggtgacgtcg atgagtttcg ccacttcttc
     3001 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     3061 atcttgcgat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     3121 ggacttaaac tccggcgtct gcgtgtttgc atcaccaacg aatggcgtta acgtattggc
     3181 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     3241 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     3301 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     3361 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     3421 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     3481 cttatcggct ttacccaatg cttcggcgtc gtctttaaag attcgcgcag ccgggttcga
     3541 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     3601 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     3661 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     3721 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     3781 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     3841 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacggcgtg ccatctggtt
     3901 gccttccggc gtccagctac cggcgaaaat ccagcaacca caggaagttg taccgtcatc
     3961 gcgcagttgg gcaaacgagc taagttgttg gcctttcttg acgataaccg cacctgttgc
     4021 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     4081 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctggtccg ggttcgcgcc
     4141 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcgccatc
     4201 agtcagcgca atccctgggg catccgcacc tttccagtgc cactgcaacc agcgacctga
     4261 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     4321 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     4381 ttcagtgttc agcgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     4441 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     4501 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     4561 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     4621 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     4681 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     4741 catgtaacct ggcaggctct gcgacagcag ccccaggtcc gtcagcccct gaatattgga
     4801 gtgaccgcgc agggcgttaa caccgccgcc agccatcccc atgttaccga gcagcagctg
     4861 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     4921 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     4981 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     5041 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     5101 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     5161 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     5221 ggcgttggta taggcttcag tgtattcgcg gttgaatttt tcattgttca gcaggtacag
     5281 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     5341 caccgcagcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     5401 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     5461 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     5521 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     5581 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     5641 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     5701 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     5761 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     5821 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     5881 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     5941 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     6001 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     6061 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     6121 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     6181 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     6241 aaaggtccag aattggctca atttccctcc atccaggaag ggtttgtaac agaataccat
     6301 aatgttggtg tgtgtgttct tatctggtta agagaaagtg aaaaaaacac agcaaaaaga
     6361 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     6421 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     6481 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     6541 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     6601 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     6661 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gagtggagca
     6721 acttaatgac atcggaaaac cggtgcagcc gctgccgttc acccagacgt ttgatctcaa
     6781 caaactggat gatgcattac gtcatctcaa tgatttccag ccagtggggc aactgaccgg
     6841 ttgtactcac gccgccgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     6901 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     6961 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     7021 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     7081 agtggctgag cgctgtaatt tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     7141 ttatacccat ccgcagcgtt tgagcaat
//
LOCUS       DAZUCE010000241         7160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZUCE010000241 DAZUCE010000000
VERSION     DAZUCE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.241"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..4526)
                     /locus_tag="ACHY03_004359"
     CDS             complement(<1..4526)
                     /locus_tag="ACHY03_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS3907518.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVKINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNASFMDRVSA
                     GASLLFPSGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARQGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLRFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVTVTGAEGYYNATLDYGDDCTTVTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWQ
                     YCYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAGTGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYLYILDNCGRVTEERDWGGVVCRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEIITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQLTSQRAGLFPEEATWGGLLPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSR
                     DQMSGYCSAQGEQWEYRYDASGRRTEKCCRQKAIRITYLWDGDSIAEIREYRDDELYS
                     VRHLVFNGFELISQQFSRVRQPHPSVAPQWVTRTNHAVSDPTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTDDPDPRGELDAEADPGLLYAGQWQDTESGLCYNRFRYYEPE
                     TGMYLVSDPLGLQGGEQTYRYVPNPCGYIDPLGLAICQLARWTKWGSEQSNISDVLNS
                     LGNRALKYANGDWI"
     gene            complement(4546..5007)
                     /locus_tag="ACHY03_004360"
     CDS             complement(4546..5007)
                     /locus_tag="ACHY03_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFS3907519.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5035..6936)
                     /locus_tag="ACHY03_004361"
     CDS             complement(5035..6936)
                     /locus_tag="ACHY03_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFS3907520.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MEGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGAGTALDNHFAVIPADRTWRPQPRLKPLVDGPQSAVVTGPEGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGKEQVYIHAQKDMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLMVEKGDKITGVRKGSLSENVFQDRGTIAGS
                     VHVDAVDNGGEGNGIQAYTAIKEIMLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1319 a         2204 c         1941 g         1696 t
ORIGIN      
        1 tttatccaat caccattagc atatttaagt gctctattcc ctaatgagtt caaaacatca
       61 gatatgttgc tttgctcact cccccatttc gtccagcggg ctaactgaca tatagccagc
      121 cccaaaggat cgatataccc acaaggattc ggcacatacc ggtaagtctg ctcccctccc
      181 tgcaacccca gcggatcact caccaggtac attccggttt ccggctcgta ataccggaac
      241 cggttatagc acagcccgga ttccgtatcc tgccactgtc ctgcatacag caggccgggg
      301 tcggcttccg cgtccagttc cccgcgcggg tccgggtcgt cggtgtctgc gggcaggctg
      361 agtgccagcc cccacaggct ggtctgtccc ggtcgccaga ccgtcttgcc ttcactgtta
      421 aagagcatca gcgggcggcc cgtcgggtcg ctcaccgcgt gattcgtccg cgtcacccac
      481 tgcggggcca cggacgggtg cggctgccgt acccggctga actgctggct tatcagctca
      541 aagccgttaa acaccaggtg ccgtacgctg tacagttcat catcgcggta ttcccggatt
      601 tccgcaatac tgtcgccgtc ccacagatat gtaatgcgga tggctttttg ccggcagcat
      661 ttttccgtcc gcctgccgct ggcgtcgtag cggtattccc actgctcacc ctgtgcgctg
      721 caatacccgg acatctggtc acggctgtcc caccggaacc gctctgtttc cgggcggtag
      781 ccgtcacggt gttttgtccg gctgaccatc cggcctgcgg cgtcatagtc atactgcgtg
      841 tttccggcct gtttcagccg gtggcctccg gcatactggt cagtctcttc acttatccgg
      901 tgcctgcccg cagactgcgc cttcaggtag ccacaactgt catagccata ggactcatcg
      961 ccttcgccat agcccagccc ggtgcctgag gctgtcaccg ccgtgacctg accgttccgg
     1021 tccagccggt attcccgccg tgtttcccgt ccgtaatctt cccggctggt gacagccgac
     1081 acattgcccg cgctgtcgta acggtactcc cgtaccagtg aggggagcag cccgccccac
     1141 gtggcttcct ccgggaaaag tccggcgcgc tggctggtaa gctggccggc tgcattccag
     1201 ccctgctcac aggcaaaacc ccggttactg tgccggagca cttcacgtcc cgcctcatca
     1261 cggctgaacg tcagcgtctc atcccccggc agaatgacct ccgtcagttc tccggcggcg
     1321 ttataacggt acgtggtttt cagcagggat tcatcggggt gtctgtcgcg ttcatcttcc
     1381 ggtggcagga tacagcggat gacggtgtgt tcgtcggggt agtggtattc aatggcccgt
     1441 cggccctgag tggtgacatt cacccgcccg cgttcgtcat agccggtgcg ctgtgatgtg
     1501 ccgtccgggc tgaagatacc cgtcagcctg ccggatttgt catacgcata ctgcgttttg
     1561 ccttccggcg tgatgatttc tgccaggcgg cctgcggcgt cccggctgta gaggatagtt
     1621 tcctccaggc cgttgaccct ggcggtacac aggccatcag cgtcataacg gtaacgacag
     1681 accacgccgc cccagtcacg ttcttccgtc acccggccac agttgtccag aatataaaga
     1741 taagactcgc cctgcgggtt aatgacttct gtcagttgca gcgtgtcttt atcgtagcgg
     1801 tactgccagc gatggccttc accgtccgtt cgcgccaccg gcaaatcaaa cgggccgtat
     1861 tccattgtcc agacaacgcc gttaccgtcc gtgtacgcca gcaggttgcc gtggcggtcg
     1921 tagcggaatc cttctgcgcc ggttcctgcc cgctcaaggc cgctcagttt atggtgctgc
     1981 tgccactgca accgcactga ctgaccgccg gggccgcgca tcttcaccgg caggtcgtga
     2041 ccgctgtatt cggtgtggtg ttcacggccc atcacgtcgg tgagtgtttc cggccggttg
     2101 tggtggtcgt actctatgcc cagacgccgt tcatcctggc ggaatatccc cgacagcagc
     2161 ccctgcggcg tgtacgccag ccgggtctct tcgccctgtg gcgacaccag acttaccggc
     2221 aggccgcgtt catcacactg ctgcgtccac gtctgcccgg cagggtcggt gatgttgctg
     2281 agcaggccgc gctcatcata gcagtactgc cactccgcgc cgtctgccgc cgtgtaacgg
     2341 cttaactgac cgtgtgcggc gttgtattca aacttctcca cccgcccggc aggggagtgg
     2401 cgggccagca ggtgatggaa ttcatcccat tcatacgtgg tggtactgcc gtccggcgct
     2461 tcttcccgca gaatattgcc gtcaggatca tagtaataac ggtgagtgcc cttgccgtcc
     2521 gtcacggtgg tgcagtcgtc cccatagtcc agcgtggcgt tgtaatagcc ctccgccccg
     2581 gtgacggtca cgcaccggcc ctgtgcatcg taggtgaaac ggctccacgt ctggtcgtta
     2641 tcggaccagc ggatgatccg gtccgcagcg tcgtactcat aaaacaggtg gtagtccagc
     2701 cgcgcatccg cttctgtcag ccgcccccgc gcatcctgtt cgtaggtcgc cagcgtctgt
     2761 atgccgttat ccgtgcggtg aatggctttc aggtagccgc tggcgtggta cgtcagttta
     2821 agccggatcc cgtcggtgtg actgacaccc gtgatccggt actggctgtc gcgctcaaac
     2881 gtgatgcggt tgctggcggt gtcgctcatc ccgcccagca tgtacatccg gtctgcgggg
     2941 ctgtgtgcca ggcggtcatc gctggtatcg tcagtcaccg ggtggcgcag acgcattccc
     3001 ggaaaggcgt cataaaagaa gcggctgctc agtgcatccc tgtcgaacag ctccagataa
     3061 tcatcgcggc ggcgcagaat gtactgggcg tgatacgggt taaccgccgt atcactgtca
     3121 ccgtcgaagg caaatctcag cgtggctccc tggctgatga tatccacccg gtttccctgt
     3181 tcacgcaccc aggcgtattc gctccaggag tcgctccagc ccacgcccgt caggccatgc
     3241 agtggcagta ccgaactgtg gaaacgggtg aaggagaggg ggagggtctg gcccagggtg
     3301 aagtcggtac gttcgtcgca gtacgcgccg gttaccggat cgacaggatc tgttacccat
     3361 ctgatgactt tattggcaat actttttaac gacttacctg ttttccccag caacgcgccg
     3421 agctttgccc ccagcagctt cccgcttttc cccagcaggt ttcccagttt gcccagtttc
     3481 gccagattcc ccagttttcc cagcagtcct gctggcggca gaagatagct ggctatcgtt
     3541 attcctccca ggatcattct ctgccatggc gggaactccg gctggatatc cagataggtg
     3601 acctgctcac ccccaataaa gacgtccggc gaaccttcca cgatcgccgc actgcactcc
     3661 agtttgtccc cctgacgcgc ggcaggctga ttgttgataa agacgttgct gctgccctgg
     3721 gctattctcg gtggtttgtt ctccttgtcg cagagcgccg tgtcctcacc ggcttccggg
     3781 aaatccacat cttttttgat ttcgccacgc ccgcccacca tcgtggcaaa ttccattaat
     3841 accgggccgc tgggaaacag aagcgatgcg cctgcactga cgcgatccat aaaactggca
     3901 ttgctgtccg tcagggtgat aacagcgttc ccccaggcct tcgccagtgg tactgctttt
     3961 tccgctgccg attcaagcag gtcagtggca atatcacccc atgagggttc cccctgtggg
     4021 acttcaggta ttgctccagg tgggggagga gggggaaggg tgacggctgc acgggctgct
     4081 ggctttccgt taatttttac gttttcagaa ccggtattga tggcgccgtc cgtgtcacaa
     4141 aagctgttta tccatgattc aagctggttc tgtttctcct ccacataatc actcgcctta
     4201 tagctggcat acatcagcgc aggcaccaga accagcgcat aagggccggt ggcacaggcg
     4261 gccataatca ggcaaccttc tgcgatggtc actatcgccc ccagcacggc ccctgccagc
     4321 gcccccaaaa aactcttgtg ctgaatttca tcgcccagcc tggcggcggg ggggccagac
     4381 ttttttgatg ccagcatctg tgcaatggct ttgtcgttgg ctgcccgttt catctggctg
     4441 gcgaccatcg cctgctgtga cagcattcgc attgccagcg taccgcctgc ggtcgctccc
     4501 tgtggcccgc ctggtccttc actcatgtgt tatccctcgt ttatgtcagc gcggctggtc
     4561 cttttggatg tcattctccg gtggcaacgg ggtgaagctc tttatcacct ccagcagcat
     4621 cgtcttttga ctgttcttca gggataagga ggtgctgaaa gtgaaggtca gcagcgtatc
     4681 tccacgccgc tggaatacgg ctatctggct aatccgcccc tgattccctt catcttccgg
     4741 accgttccag gaataatcca gcagccaggc cggggcctct gccagagtga tttcccggca
     4801 ggcatgttcg gtgtacccat caagtttgcc ctggtacagc gccagcgttt cctggtacag
     4861 ctctggtgtc agggttccct gtggaacggc ttcgcgggta atgaccaggt tgatgccact
     4921 gttatctgtg gacaccagga cctgcattga ctgatcctgc cattgagacg gcagttgaat
     4981 gtggccttca ttgaaagtga actgcataaa atactccctg ataatctgag tgaatcagtt
     5041 gatgaaaaca gaaccgccca cgatggtgat gccatccttc gacagcctga ttaccgttga
     5101 ttccccgacc tgtagctgaa tgccatccgg cgtcagcgca attttgcttt cctccacggc
     5161 cagcataatt tccttaattg ccgtataagc ctgtataccg ttgccttcgc caccattgtc
     5221 tacggcgtca acatgcaccg aaccggcaat cgtgcctctg tcctgaaaga cattctcaga
     5281 taaagaacct tttcgtactc ctgtgatctt gtcgcctttt tccaccataa gaaggcgatt
     5341 accttcctgt atcgtgaccg tctggtcatt ttttacggtt tcgcgatgat cgtgttttac
     5401 ggtggtggta cgatcgttga gtacctcggt atccatgtcc ttctgcgcgt ggatgtagac
     5461 ctgttctttc cctgtcgcat cgtcaaactt cagctcatta aacccgctgc ccatatacgt
     5521 tttggaacgg atggtcatct gcgtcttcgt ccccggcagg ctgccggggg tgcggttttc
     5581 ctggtggtag gtgcgcccca taatgatcgg ctggtccgga tcgccgttga ggaagtccac
     5641 tatcacctcc tgacccacac gcggtatcgc caggttgcca aaaccggtgc ctgcccacgc
     5701 ctgtgccaca cggatccagc acgagctgtc ctggtctgcc gggttataac ggtcccagtt
     5761 gaatttcacc cgcactcggc catgttcatc acagaagatt tcctcaccct ccggccccgt
     5821 cacgacggcg ctctgcggac cgtccaccag cggttttagc cgcggctggg gtcgccatgt
     5881 ccggtctgcc gggatcaccg caaaatggtt gtccagcgcc gtccctgctc cccgccttcc
     5941 cggcaccgcc tgcggctgct cgccgtgcag ttcacttgcc accacctgcc attcccggtt
     6001 caggttcgcc tgcggatgcc ccgtcagcac aattcgccgt cccggccata tctccggcga
     6061 gcgactcatt ccccgcgcgg tttctgcatt gtttcgccag ccctccatct gccagcgggc
     6121 aaagttctgc ccgtgggcgc ccttgaaacg tccggggtag tcatacacct catactgcgt
     6181 gcgctggtaa tcctggtgct ggccttcctg atcaaaacgt cctgcccagc cggggcgttt
     6241 aaaggtgtag tctttggtca ccacggaaga agggcggatt tgtgcgctgt agcgaaactg
     6301 gctgatgcag agggtgctca cctcggtacg ggtgttcggg ttccagggga tttcaaaaga
     6361 ttcgggcaga tggcggaccg tgtcgcacag caccaggctc tggtcggtac ttttgtaagc
     6421 atgctcctca taaaagaaga tgccttcctc cgccgccatc cggcacagga aatcgtaatc
     6481 agtctcaccg tactggacac aaaactcacg ggaaggatgc ggttcactga acagcggact
     6541 ccactcggtc accccgtttt cctgcaatat cgtgccgagg atgcttttga tgtcctcgtt
     6601 ctggaagata cggaagttct ggcgcagacc ggcacgccac agcggcgggt gcaccttcat
     6661 gctgtacagc atctggtttt tgtcgttctc ccccagttca aaccaggtca ccacaccttt
     6721 cacccggcgc tgtacatcat caccctgcca tattgtcagg taggccattt tgtccagcac
     6781 ctgcgcaaat tcaagggaga gaaactgctg gctgaccagg gagagatcga gggaaaagag
     6841 cgaagagagt gactgggtga gatgaaagga aaccaccgca aaagcgtccg gtggcaggcc
     6901 gtccacttcc agtgtgaaac gtaatccggt tgacatagca acctccggta aaatgaataa
     6961 taatccgcac cgtacaactg cggataatga gataagcggg acgacttaag acgttattag
     7021 ggaaggaaca aaaaatgcta tgattatgtg agaattttgt ttgaatatta ctttacagaa
     7081 acgggtgtct gttcagtcaa aaaaataatc acaagctcaa cttattgttt cctgacgtta
     7141 ttcaggacag gtgcgcacat
//
LOCUS       DAZUCE010000242         7157 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZUCE010000242 DAZUCE010000000
VERSION     DAZUCE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.242"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            95..1429
                     /gene="cadB"
                     /locus_tag="ACHY03_004362"
     CDS             95..1429
                     /gene="cadB"
                     /locus_tag="ACHY03_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFS3907521.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            1509..3656
                     /gene="cadA"
                     /locus_tag="ACHY03_004363"
     CDS             1509..3656
                     /gene="cadA"
                     /locus_tag="ACHY03_004363"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFS3907522.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3715..5172
                     /gene="dtpC"
                     /locus_tag="ACHY03_004364"
     CDS             3715..5172
                     /gene="dtpC"
                     /locus_tag="ACHY03_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFS3907523.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMVQESND"
     gene            5409..6926
                     /gene="lysS"
                     /locus_tag="ACHY03_004365"
     CDS             5409..6926
                     /gene="lysS"
                     /locus_tag="ACHY03_004365"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFS3907524.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(7045..>7157)
                     /gene="ghoT"
                     /locus_tag="ACHY03_004366"
     CDS             complement(7045..>7157)
                     /gene="ghoT"
                     /locus_tag="ACHY03_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFS3907525.1"
                     /translation="IWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
BASE COUNT         1761 a         1675 c         1756 g         1965 t
ORIGIN      
        1 cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat agttatacat catgacccgg
       61 actccaaatt caaaaatgaa attaggagaa gagcatgagt tctgccaaga agatcgggct
      121 atttgcctgt accggtgttg ttgccggtaa tatgatgggg agcggtattg cattattacc
      181 tgcgaaccta gcaagtatcg gtggtattgc tatctggggt tggattatct ctattattgg
      241 tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca aaaaacccgc aacaaggtgg
      301 cccaattgct tatgccggag aaatttctcc tgcatttggt tttcagacag gtgttcttta
      361 ttaccatgct aactggattg gtaacctggc gattggtatt accgctgtat cttatctttc
      421 caccttcttc ccagtattaa atgatcctgt tccggcgggt atcgcctgta ttgctatcgt
      481 ctgggtattt acctttgtaa atatgctcgg cggtacttgg gtaagccgtt taaccactat
      541 tggtctggtg ctggttctta ttcctgtggt gatgactgct attgttggct ggcattggtt
      601 tgatgcggca acttatgcag ctaactggaa tactgcggat accactgatg gtcatgcgat
      661 cattaaaagt attctgctct gcctttgggc cttcgtgggt gttgaatccg cagctgtaag
      721 tactggtatg gttaaaaacc cgaaacgtac cgttccgctg gcaaccatgc tgggtactgg
      781 tttagcaggt attgtttaca tcgctgcgac tcaggtgctt tccggtatgt atccgtcttc
      841 tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct tcaactatcc tcggtaactg
      901 ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc ctgacttctc tgggctcctg
      961 gatgatgttg gtaggccagg caggtgtacg tgccgctaac gacggtaact tcccgaaagt
     1021 ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt ctgctgctgg ctgcagtgaa
     1081 aatgactgcc ctgatgatcc tcatcactct gatgaactct gccggtggta aagcatctga
     1141 cctgttcggt gaactgaccg gtatcgcagt actgctgact atgctgccgt acttctactc
     1201 ttgcgttgac ctgattcgtt ttgaaggcgt taacatccgc aactttgtca gcctgatctg
     1261 ctctgtactg ggttgcgtgt tctgcttcat cgcgctgatg ggcgcaagct ccttcgagct
     1321 ggcaggtacc ttcatcgtca gcctgattat cctgatgttc tacgctcgca aaatgcacga
     1381 gcgccagagc cactcaatgg ataaccacac cgcgtctaac gcacattaat taaaagtatt
     1441 ttccgaggct cctcctttca ttttgtccca tgtgttggga ggggcctttt ttacctggag
     1501 atatgactat gaacgttatt gcaatattga atcacatggg ggtttatttt aaagaagaac
     1561 ccatccgtga acttcatcgc gcgcttgaac gtctgaactt ccagattgtt tacccgaacg
     1621 accgtgacga cttattaaaa ctgatcgaaa acaatgcgcg tctgtgcggc gttatttttg
     1681 actgggataa atataatctc gagctgtgcg aagaaattag caaaatgaac gagaacctgc
     1741 cgttgtacgc gttcgctaat acgtattcca ctctcgatgt aagcctgaat gacctgcgtt
     1801 tacagattag cttctttgaa tatgcgctgg gtgctgctga agatattgct aacaagatca
     1861 agcagaccac tgacgaatat atcaacacta ttctgcctcc gctgactaaa gcactgttta
     1921 aatatgttcg tgaaggtaaa tatactttct gtactcctgg tcacatgggc ggtactgcat
     1981 tccagaaaag cccggtaggt agcctgttct atgatttctt tggtccgaat accatgaaat
     2041 ctgatatttc catttcagta tctgaactgg gttctctgct ggatcacagt ggtccacaca
     2101 aagaagcaga acagtatatc gctcgcgtct ttaacgcaga ccgcagctac atggtgacca
     2161 acggtacttc cactgcgaac aaaattgttg gtatgtactc tgctccggca ggcagcacca
     2221 ttctgattga ccgtaactgc cacaaatcgc tgacccacct gatgatgatg agcgatgtta
     2281 cgccaatcta tttccgcccg acccgtaacg cttacggtat tcttggtggt atcccacaga
     2341 gtgaattcca gcacgctacc attgctaagc gcgtgaaaga aacaccaaac gcaacctggc
     2401 cggtacatgc tgtaattacc aactctacct atgatggtct gctgtacaac accgacttca
     2461 tcaagaaaac actggatgtg aaatccatcc actttgactc cgcgtgggtg ccttacacca
     2521 acttctcacc gatttacgaa ggtaaatgtg gtatgagcgg tggccgtgtg gaagggaaag
     2581 tgatttacga aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga
     2641 tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca
     2701 ccacttctcc gcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag
     2761 gcaatgcagg taagcgtctg atcaacggtt ccattgaacg tgcgatcaaa ttccgtaaag
     2821 agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtatgg cagccggatc
     2881 atatcgatac gactgaatgc tggccgctgc gttctgacag cacctggcac ggcttcaaaa
     2941 acatcgataa cgagcacatg tatcttgacc cgatcaaagt caccctgctg actccgggga
     3001 tggaaaaaga cggcaccatg agcgactttg gtattccggc cagcatcgtg gcgaaatacc
     3061 tcgacgaaca tggcatcgtt gttgagaaaa ccggtccgta taacctgctg ttcctgttca
     3121 gcatcggtat cgataagacc aaagcactga gcctgctgcg tgctctgact gacttcaaac
     3181 gtgcgttcga cctgaacctg cgtgtgaaaa acatgctgcc gtctctgtat cgtgaagatc
     3241 ctgaattcta tgaaaacatg cgtattcagg aactggctca gaatatccac aaactgattg
     3301 ttcaccacaa tctgccggat ctgatgtatc gcgcatttga agtgctgccg acaatggtaa
     3361 tgactccgta tgctgcattc cagaaagagc tgcacggtat gaccgaagaa gtttacctcg
     3421 acgaaatggt aggtcgtatt aacgccaata tgatccttcc atatccgccg ggagttcctc
     3481 tggtaatgcc gggtgaaatg atcaccgaag aaagccgtcc ggttctggag ttcctgcaga
     3541 tgctgtgtga aatcggcgct cactatccgg gctttgaaac cgatattcac ggtgcatacc
     3601 gtcaggctga tggccgctat accgttaagg tattgaaaga agaaagcaaa aaataattag
     3661 ctcgtacaag ggaagtggct tgccacttcc cttttttgct cataaggaga acacatgaaa
     3721 acaccctcac agccgcgcgc gatatactat atcgtggcga tccaaatctg ggagtacttc
     3781 agtttttacg gcatgcgtgc cttactcatt ctctatctca cccatcagct tggttttgat
     3841 gataaccatg ccatcagcct gttcagcgca tatgcttctc tggtttacgt tacccctatt
     3901 ctcggcggct ggcttgccga ccgcctgctc ggcaaccgca ctgcagtgat tgccggcgcg
     3961 ctgttaatga cccttggcca tgtggtgctg ggcattgata caaattcaac ctttagcctg
     4021 tatctggcgc tggcaatcat tatttgtggc tacggtttat tcaaatcaaa catcagctgt
     4081 ttgcttggcg agctctacga cgagaacgat catcgacgtg atggcggttt ttcgctgctg
     4141 tatgctgcgg gcaatatcgg ttctatcgcc gcccccatcg cctgcggcct ggctgctcag
     4201 tggtatggat ggcatgttgg ctttgccctt gcgggtggcg gcatgtttat cggtttgttg
     4261 attttcttaa gcggacatcg tcatttccag tccacgcgta gtatggataa aaaagcgctc
     4321 actagcgtca aatttgcctt accagtatgg agctggttag tggtgatgct ctgtttagcc
     4381 ccagtatttt ttaccctgct gctggagaac gactggtcgg gatatttgct ggcgatcgtt
     4441 tgcctcattg ccgcacaaat cattgcccgc atgatgatca aattccccga acaccgccgt
     4501 gctctttggc aaattgtatt gttgatgttt gtcgggacat tgttctgggt actggcacaa
     4561 cagggcggca gtaccatcag cttgtttatc gatcgctttg tgaatcgtca ggcattcaat
     4621 attgaagtac ctacagcact attccagtcg gtgaatgcaa ttgcggtgat gctcgctggg
     4681 gttgtactgg cctggctggc atcgccagaa agccgcggca actcaacatt gcgcgtctgg
     4741 ctgaagtttg cctttggctt acttctgatg gcttgtggct ttatgttgct ggcatttgat
     4801 gcccgacatg cagcggctgg cggtcaagcg tcaatgggcg tgatgatatc cgggctggcg
     4861 ctaatgggct ttgccgaact ctttattgac ccggtggcga ttgcgcaaat cacgcgtctg
     4921 aaaatgtctg gcgtattaac cgggatttat atgctggcaa caggcgcggt cgccaactgg
     4981 ctggcaggcg tagtggcaca gcagacgaca gagtcgcaaa ttagcggtat ggcaattgca
     5041 gcttaccagc gattcttttc tcagatggga gagtggacgt tggcttgtgt cgcgatcatc
     5101 gtcgtattgg cctttgctac ccgtttcctg tttagcacgc cgacgaatat ggtacaggag
     5161 agcaacgatt aactgtcgta aaaacaattg gttatgaata aattaacttt cagtttatca
     5221 cactagtaaa tcgagattaa taagtcattt aaaccatttt gatggttatt tattagtgat
     5281 atcaacttga ggtaagcgtt agtttcgata agataaactg agttattaat agtcgaggca
     5341 gataatacag tgtaccgatc tgtctcttta tctacgctaa attgaaagct ggatttagag
     5401 gaaccaaaat gtctgaacaa gaaacacggg gagccaatga ggctattgat tttaacgatg
     5461 aactgagaaa tcgccgcgaa aaactggcgg cactacgtca gcaaggtgtg gcgtttccca
     5521 atgattttcg ccgcgaccat acctctgacc agttgcacga agagtttgat gcgaagaata
     5581 accaggaact ggaatcctta aacattgaag tctcggttgc tggccgaatg atgacccgtc
     5641 gtatcatggg gaaagcctcc tttgtaacgt tgcaggatgt cggtggccgt attcaactgt
     5701 acgtcgcacg agatagcctg ccagaaggtg tttataacga tcagtttaaa aaatgggatc
     5761 tgggtgacat tatcggtgcc cgcggtacgc tgtttaagac gcaaacgggt gagctttcca
     5821 ttcactgtac tgagttgcgc ctgctgacta aagcactacg tcctttacca gataaattcc
     5881 atggtctgca ggatcaggaa gtccgttatc gtcaacgtta tctggacctc atcgctaacg
     5941 ataaatcccg tcaaacgttt gttgtccgtt caaaaattct ggccgctatc cgtcagttca
     6001 tggtcgcgcg cggctttatg gaagtagaaa ccccgatgat gcaggtaatt ccaggtgggg
     6061 catctgctcg cccatttatt acccatcata atgctctgga tttagatatg tatctgcgta
     6121 tcgcgccgga gctgtatctg aaacgtctgg ttgtaggcgg ttttgaacgg gtattcgaaa
     6181 tcaaccgtaa cttccgtaat gaaggtattt ctgttcgcca taatcctgag ttcacaatga
     6241 tggaactcta catggcgtat gcggattacc acgatttgat tgaactgaca gagtcactgt
     6301 tccgcaccct ggcacaagag gttctgggta ccactaaagt cacttatggc gagcatgtgt
     6361 ttgatttcgg caaaccgttt gaaaaactca ccatgcgcga agcaatcaaa aaatatcgtc
     6421 cagaaaccga tatggccgac ctggataatt ttgatgctgc taaagcatta gctgaatcta
     6481 tcggaattac ggtagagaaa agctgggggc tgggacgtat tgtcacggag atctttgatg
     6541 aagtggcaga agcacatctg attcagccaa cctttattac ggaatatccg gcagaagtgt
     6601 ccccgctggc gcgccgtaat gatgttaacc cggaaatcac cgaccgtttt gaattcttca
     6661 tcggtggtcg tgaaatcggt aatggtttta gcgaattaaa cgacgcagaa gatcaggctg
     6721 aacgtttcca ggaacaggtt aacgctaaag ccgcaggtga cgacgaagcc atgttctatg
     6781 acgaagatta cgtgactgcg ctggaatatg gtctgccgcc aaccgctggt ctgggtattg
     6841 gtatcgaccg aatgattatg ctgtttacaa acagtcatac tattcgtgac gttattctct
     6901 tcccggcgat gcgcccacag aaataaattt cactttaatg aacgaagcag tcaggcgact
     6961 gcttcgttca tcgttccgca aatccagttt tgaggagaga cgcttcgttt caagataacc
     7021 agcgggtcca ctcaactaca gctcctaaaa gagagaaaaa agtaatgcca caggcagact
     7081 cattggccag gttattccga ccaggaatgc actaagtaaa cgaatatgtg ttttctcatg
     7141 tgagataaac cagataa
//
LOCUS       DAZUCE010000243         7140 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZUCE010000243 DAZUCE010000000
VERSION     DAZUCE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.243"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..376)
                     /locus_tag="ACHY03_004367"
     CDS             complement(<1..376)
                     /locus_tag="ACHY03_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFS3907526.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHATLVEN"
     gene            complement(389..2170)
                     /gene="gcl"
                     /locus_tag="ACHY03_004368"
     CDS             complement(389..2170)
                     /gene="gcl"
                     /locus_tag="ACHY03_004368"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFS3907527.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2260..3075)
                     /gene="allR"
                     /locus_tag="ACHY03_004369"
     CDS             complement(2260..3075)
                     /gene="allR"
                     /locus_tag="ACHY03_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFS3907528.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3153..3635)
                     /gene="allA"
                     /locus_tag="ACHY03_004370"
     CDS             complement(3153..3635)
                     /gene="allA"
                     /locus_tag="ACHY03_004370"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFS3907529.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3865..4791
                     /gene="allS"
                     /locus_tag="ACHY03_004371"
     CDS             3865..4791
                     /gene="allS"
                     /locus_tag="ACHY03_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFS3907530.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            4860..5954
                     /gene="mnmH"
                     /locus_tag="ACHY03_004372"
     CDS             4860..5954
                     /gene="mnmH"
                     /locus_tag="ACHY03_004372"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFS3907531.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            6070..6435
                     /gene="ylbG"
                     /locus_tag="ACHY03_004373"
                     /pseudo
     CDS             6070..6435
                     /gene="ylbG"
                     /locus_tag="ACHY03_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(6956..>7140)
                     /locus_tag="ACHY03_004374"
     CDS             complement(6956..>7140)
                     /locus_tag="ACHY03_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907532.1"
                     /translation="REQVAKIVSENDPDTKKVWCKYGKIPGQGDSVNLFFVGEINVTH
                     YFITNIGAGLPDACAE"
BASE COUNT         1806 a         1860 c         1732 g         1742 t
ORIGIN      
        1 ggttttctac aagcgttgcg tgaatctgtt cactgctgaa accagtcggc gttttaccga
       61 ccagacagtt aattttttta ttacccagcg cacgagcata acgaatcgct gctgctacgc
      121 catcccgaaa ctcttcttca cggccaggaa tacaggcaat accgcgctcc cccgccgccc
      181 agtcacccgc cggtaaattg tgcagcgtat gttcgagttt attactcgcc agcacctgtt
      241 ttaattcttc aatgtcgtag tcataaggaa acataaattc aacgccgcga aaaccacact
      301 gcgcagcttt ctcaaaacgg gcgagaaaat catattcacc aaataacatc gataaattag
      361 cagagaaacg taacattatt tatctccctt attcatagtg catgaagcag gtttcagtcg
      421 gtgcatccgc tgcgttatcg gcgatatctt caaattccat gacgttatcc agttcgctgc
      481 ccatcgaaat atttgtcaca cgctcgagaa taacttccac gactaccggt acccgatatt
      541 gcgccattaa ggctttcgcc tgttcaaagg ccggtgcaat atcttccggt ttgaagacgc
      601 gaatagcttt acaacctaaa ccttccgcta cttttacgtg gtcgacgccg taaccgttca
      661 cttcgctgga gttgatattc tcgaaagcga gttgcacgca gtagtccatg tcaaaggcgc
      721 gttgtgactg acgaatcagg ccgagatagg cgttattcac cagcacatgg atgtacggaa
      781 tgttgaactg cgcgccaacg gctaactctt caatcaggaa ctggaagtcg aagtcaccgg
      841 aaatcgccac cacattgcgt ttcggatcag cggcacaaac ccccagcgcc gccggaatcg
      901 tccagcctaa cggaccagcc tgaccacagt tgatccagtg gcggtcttta aagacatgca
      961 gcatttgtgc cgcagcgatt tgtgacagac cgatggtggt gacataacaa acatcgcgac
     1021 caaaggcttt gttcatctct tcatacacgc gctgcggttt caccggcacg ttgtcgaagt
     1081 gggttttgcg cagcaaagtg cgtttgcgct gctggcagtc ggcgacccat tctttacgac
     1141 acggcagacg acccgctttt tgcatctcct gcgccacttc aaccagcagt gtcagcgccg
     1201 ctttagcatc agagacaatg ccgagatccg gacacagcac gcggccaatt tgcgtcggct
     1261 caatatcgat atgaacgatt ttgcgccctt cggtgtattt ctctaccgaa ccggtatgac
     1321 ggttagcaaa acggttaccg ataccaaaca ccatgtcgga cgccagcagt gttgcattac
     1381 cgtaacgatg cgcggtttgc agacccacca tcccggccat cagttcatga tcgtccggga
     1441 tacagcccca gcccatgagc gttgggatca ccggaacgct ggtcagttca gcaaactgtt
     1501 gtaacagtgc agctgcgtca gcattgatta ccccgccccc ggcaacaatc accggacgtt
     1561 cggcctggat taacatttct acagcttttt cgatctgcat acggctggca gcaggtttgt
     1621 agaccggcag cggttcgtac atgtcaggat caaactcgat ttccgcaacc tgaacgtcga
     1681 acggtaaatc caccagtacc ggacccggac gaccagaacg catcaggtga aatgcctgtt
     1741 gcagcacgcg aggcaccagc gccgcttcac gtactgtaac cgccattttg ctgaccggtt
     1801 tagcaattgc ttcaatatca acagcctgaa aatcttcttt atgcagacgg gcgcgcggtg
     1861 cctggccggt aatgcacaga ataggaatgg aatcagcaga agcggaatag agcgcggtga
     1921 tcatgtccgt gcccgcagga ccggaagtcc ccagacatac gccgatattc cctgccgttg
     1981 cgcgggtata accttccgcc atgtgcgaag caccttccac atgacgcgcc agaatgtgac
     2041 gaataccgcc gtgcttacgc atcgctgagt agaacggatt gattgcagct cccggaacac
     2101 cgaaggcggt ggtgatacct tctttctcca gcacatacat tgccgcgtca acggctctca
     2161 tttttgccat tttattccta cctctattaa ttggaaaaat tttccaactt tcaaattttg
     2221 tcaaaaaaac ccgctccgca gagcgggatg cgacagacat tatggatgtg ctttcagtcc
     2281 caacgccgtg ctgatatcgc gggcggtgtc tctgaccagc tcaccctgac tgacaaaacg
     2341 atcttctgtc agtcttgatg aaggcccgga gatggagata gcggcaacaa cactacctac
     2401 atcgtcataa attgctgaag ctatgcaatt caggcctaca acatgctctt ctttatctac
     2461 ggtatagccc agttcacgcg cttgttccag gtccttcagc agggtgggca tatccacaag
     2521 cgtagttggc gtaaactgct gcaaaccggt ttgcagaatg atgctcatca actcctcttc
     2581 cgccagcgga taaagcagcg ctttgcccgc accggaagca tgcagtggca gacgactgcc
     2641 cagtggcgcg cacatcctga ccatcgattt acactctaac tgaccaatta ataccgcttc
     2701 attgccgtta cggatcgcga cattgaccgt ttcgccggaa agtaacatca ggcggcgcat
     2761 aaacggtccg gcgacggaga ggacatcacg gttatggatg tacgctgcac cgacgttaaa
     2821 gacacctaat cctatatgcc accagcctaa ctgactgtcc tgatagacaa aatccgctgc
     2881 ctgtaaaacc ttcagcaagc gaaaggtcgt ggagagcggc aaatccagat tgagagaaat
     2941 atcgctaacc gacgaacttc ccccactttt ttccagatat tgcagaatcg caattccccg
     3001 ctctaatgcc tgtgcgccct tctgtgccac aggctccgcc tgtcctggcc tgccgcgccg
     3061 tctaacttcc gtcataccta cgttaactcc gacctacaag tgagcataaa caatgaacaa
     3121 ttagtttatc ttatgcaagt cggttgcagg cgtcacgcaa aacagagttc ctgttcagga
     3181 atactttcaa catcacagtt gtcactgccg ccgcgatcga tggtcagaaa atcggtgacg
     3241 cgctgccagg cgaaaagtgg gtgatgccag acgttacgat ggtaattcac tccctgttcg
     3301 ccgttggtaa taaacgcccg cagggttgac aggtctggtt tgtcgtcacc taacgccacg
     3361 accaccacaa acacctcacc tttcatcggg ataaaggcct gagtacccag cggatgacgt
     3421 tcgagttcgt gaatggtcag cggcagattc gccggttgcg cgcggttaat gctgataagc
     3481 gtacggtctt gctcaagaat ctcaaccagc gccaaatcgt ggtaacgctc caccaggcca
     3541 ttgttaatat ggaaaaaatc ccgttgctgc gtttcgatta cgtcgccata agcactaaag
     3601 gcttcctgac ttaacggtaa tacctgaagt ttcatctttt tgtccttgta acataattcc
     3661 gttcaaccac cgccgaccgg tcagcttggt ttcaataaca ggcaatctaa aaccttaaga
     3721 gaacaacatc aatacaaaag ttggaatatt tttccaaaac tagatttagt ccacaaattg
     3781 aacagggtta tcatgtaaaa atactgataa atcagaggat aagaaaatca cgcaacttgc
     3841 ggatttaaaa ggggatacct gaagatgttc gatccagaaa ccttgcggac tttcattgcg
     3901 gttgctgaaa caggaagttt ttcaaaagcg gcagaacgat tatgtaaaac cacggcgacg
     3961 atcagttatc gcattaaact tctggaagag aataccggag tagcgctgtt tttccgtacg
     4021 actcgtagcg taacgttgac agcggctggc gagcatctac tttcccaggc cagagactgg
     4081 ctgagctggc tggaaagtat gcccagcgag ctgcaacagg tgaatgatgg cgtggaacgc
     4141 caggtgaata ttgtcatcaa caacctgctc tataaccccc aggccgtcgc ccagttgctg
     4201 gcgtggctga atgagcgtta cccctttacc cagtttcaca tctcccgaca aatctatatg
     4261 ggcgtctggg actcgctatt gtacgaaggt ttttcgctgg ctatcggcgt cacgggaact
     4321 gaggcgctgg caaatacctt tagtcttgat cccttaggat cggtgcaatg gcgatttgtc
     4381 atggcggcgg atcatccgct ggcgaacgtt gaagagccgc taacagaagc gcagttgcgg
     4441 cgctttccgg cggtcaatat tgaagacagc gcccgcacct taaccaaacg cgtcgcctgg
     4501 cgattgccgg gtcaaaaaga gattattgtt cccgatatgg aaacgaaaat cgccgcccat
     4561 ctggcgggcg ttggcattgg ttttttgcca aaatcgcttt gccagtcaat gatcgataat
     4621 caacaactgg tcagccgggt aatcccaacg atgcgccctc cttcgccatt gagtctggca
     4681 tggcgcaaat ttggcagcgg caaagcggta gaagatattg tgaccttgtt tacccagcgc
     4741 aggccggaaa tcagcggatt tttagaaatt ttcggcaacc cacgcagtta aaaatcaagt
     4801 ttccttgcgc gttggcgcgt aaaatagcgc gcaagctgat ttgaggttca cttttaacta
     4861 tgcaagagag acacacggaa caggactatc gtgccctgct gattgctgat acgcccatta
     4921 ttgatgttcg cgcccctata gagtttgagc aaggcgcaat gcccgccgct atcaatctgc
     4981 cgttaatgaa taacgatgaa cgcgccgccg ttggcacctg ctataaacag caaggctcag
     5041 acgcagcgct ggcgctggga cataaactgg ttgcgggtga aattcgtcag cagcgcatgg
     5101 acgcctggcg ggcagcgtgc ctgcaaaatc cgcaaggtat tctctgctgc gcccgtggcg
     5161 gtcagcgctc acatattgtg caaagctggt tgcatgcagc ggggattgat tatccgctgg
     5221 tggaaggcgg ttataaggca ctgcgccaga ccgcgattca ggcgaccatt gaactggcac
     5281 aaaaaccgat agtgctgatt ggcggttgta ccggcagcgg taaaacgctg ttggtgcagc
     5341 aacagccgaa cggtgttgat ctggaagggt tggcgcgtca tcgcggttcg gcgtttggtc
     5401 gcaccttaca accacaactt agccaggcga gttttgaaaa cctgctggct gccgaaatgc
     5461 taaaaaccga cgcccgtcag aatttgcgcc tgtgggtgct ggaagacgaa agccggatga
     5521 tcggttcgaa tcacctgccg gaatgcctgc gcgagcgaat gactcaggcg gcgattgcgg
     5581 tggtagaaga tccgtttgag atccgtcttg agcgcctgaa cgaagagtat ttcttgcgta
     5641 tgcatcatga ttttacccac gcgtacggcg acgaacaggg ctggcaggaa tattgcgaat
     5701 acctgcatca cggactttcg gctattaagc gtcggctggg gctacagcga tataacgaac
     5761 tggctgcaag gctggatgca gcgctgacaa cgcaactcac caccggcagc accgacggtc
     5821 atctggcctg gctggtgccg ttacttgaag agtattacga cccaatgtat cgctatcagc
     5881 ttgagaaaaa agcggaaaaa gttgtctttc gcggtgagtg ggcggaagtg gcggaatggg
     5941 ttaaggcgcg gtaatgaggg acgtatttgt attagtcgcg acttgatctg acacatggcc
     6001 ttgaaaggtt gagagttacc ggttttgata tgagtgtcga atccttatcc aaaacaagag
     6061 gtaactctca tgcttcatac tgccaatccc gtcatcaaac acaaagccgg tttgctcaat
     6121 ctggctgaag aactcagcaa cgtatcaaaa gcctgtaaaa tcatgggcgt ttcgcgtgat
     6181 acgttttatc gttaccgcga actggtcgct gaaggcggtg tggatgcgca gattaatcgt
     6241 agtcgccgcg ctcctaacct taagaaccgt accgatgagg caactgaaca ggctgttgtt
     6301 gattacgccg tcgctttccc gacacatggt cagcaccggg ccagcaacga gctgcgtaaa
     6361 cagggggttt ttatctccga cagtggtgtc cgttctgtct ggttactcca taaccttgag
     6421 aatctcaaaa gacgatactg aacaaatttt atcaggtgac gttccgtaaa aagttgtatg
     6481 gagttctcga tgcagtcggc gcgactatca gtacccccgc tgaaccctgg actatcgggc
     6541 cgccgtactg gggcatgtca ccctggcgtg ccgctggttt tccgctaatg gtttatttcc
     6601 tctttctttc ccggcaagaa tttattttaa tccggaagga ttatttattt tcagataggg
     6661 actcacatta aacacgtaac attttaatta atgcatggct gaatatgtat tacacaatgt
     6721 cagatatcgt aaaagaccag tgcaattcta tgtaaactct ctgactgttt attttatttg
     6781 ttgtttcagg gtcggaactc accctcaata aacaactgaa tacgagtaga tattcatcgt
     6841 ctgagctata tggctttaca caatagccga catgtctttt ctcagtcaga aattttactt
     6901 ttcctcacta cgaagttgac gacttcgata tgggatagac tcttaattca agcaattact
     6961 ctgcacaagc atcaggcaat ccagctccaa tatttgttat aaaataatgc gtaacattaa
     7021 tttcaccaac aaaaaaaagg tttacgctat ccccttgccc tggtatttta ccatatttac
     7081 accacacttt ttttgtatct ggatcattct ctgatacgat tttcgccact tgttccctgg
//
LOCUS       DAZUCE010000244         7121 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZUCE010000244 DAZUCE010000000
VERSION     DAZUCE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.244"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            182..1033
                     /locus_tag="ACHY03_004375"
     CDS             182..1033
                     /locus_tag="ACHY03_004375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS3907533.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINTGYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLHAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGGKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1060..2049
                     /locus_tag="ACHY03_004376"
     CDS             1060..2049
                     /locus_tag="ACHY03_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS3907534.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGSIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTQLHRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2081..2974)
                     /locus_tag="ACHY03_004377"
     CDS             complement(2081..2974)
                     /locus_tag="ACHY03_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3907535.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLRKYPEISVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKTLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3175..4101)
                     /locus_tag="ACHY03_004378"
     CDS             complement(3175..4101)
                     /locus_tag="ACHY03_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3907536.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            4258..5178
                     /locus_tag="ACHY03_004379"
     CDS             4258..5178
                     /locus_tag="ACHY03_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3907537.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            5422..6565
                     /locus_tag="ACHY03_004380"
                     /pseudo
     CDS             5422..6565
                     /locus_tag="ACHY03_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
     gene            7040..>7121
                     /gene="ykgR"
                     /locus_tag="ACHY03_004381"
     CDS             7040..>7121
                     /gene="ykgR"
                     /locus_tag="ACHY03_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFS3907538.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAY"
BASE COUNT         2031 a         1670 c         1585 g         1835 t
ORIGIN      
        1 tatggatatt cattttcctt catcggactg taaattgcgg agatatcgaa cttgcactac
       61 ccatcgcaac actcaaacag atttatgaat ttcgttcata acccctgaca aatcctgccg
      121 tctaatcgag ctcttctctt tacgttatgc ttctcatgaa cgataacgga aggataacac
      181 catgcaaaaa gtaaaactga ataacggtat tgaaatgccc ctgctgggct ttggtgtatt
      241 tcagatgacc gatgccgccg aatgcgaaag agcggtcatt gacgctatta acaccggtta
      301 tcgactgatt gacaccgccg cctcctatca aaatgaaacc caggtcggga acgcactgaa
      361 acaaagcggt attgcgcgta acgagctctt tgtcaccacg aaactctggt tgcaggatac
      421 cagttacgaa ggggccaaag cccagttcga acgctctctg aatcgactgc aacttgatta
      481 cgttgacctg tatttgattc atcagcctta cggtgatgtc cacggtgcct ggcgtgccat
      541 ggaagagctg caacaggccg gtaaaattcg cgctattggc gtcagtaact tccatcctga
      601 ccgactggct gaccttatcg cctttaataa cgtcgttcca gcggtaaacc aggttgaagt
      661 taaccctttc aatcagcagc tacacgccgt tccgtggatg caaagccgtg gtattcagcc
      721 agaagcctgg gcaccgtttg cggaagggaa gaatggtctg ttccagcatc ctgttctcac
      781 tgcaattggc gggaagtatg gcaaaagcgt aggccaggtt gttctgcgct ggatctacca
      841 acgaggcatt gtttcgcttg caaaatcagt acgcaaagaa cgcatggaag agaacatcaa
      901 cattctcgat tttgaactca gtcctgaaga tatgctgcaa attaccgccc tggataccgc
      961 aaccagcgcg ttcttctctc accgcgatcc cgcaatggtg gaatggctga ctggccgcaa
     1021 acttgatgtt taagccgcga taaaagaggt attcattcaa tgcaaaaacg ttatctgggt
     1081 aaatccggac tcgaagtctc cgcccttggg ctcggttgca tgggcttaag ccacggctac
     1141 ggaccggcga ccgatacccg tcaggctatc gaacttattc gtgctgcggt tgaacgtggc
     1201 gtcaccttct tcgatacagc cgaagtgtat ggtccgtatc tgaatgaaga agtggtcggc
     1261 gaagccttaa aaccgtttcg tgaccgcgtg gtaattgcca ctaaattcgg ttttaccttc
     1321 ggcgatgata ataagcagca gattttaaac agccgtccgg aacatatccg tgaagccgtt
     1381 gaaggatcat tacgtcgtct gaagacagat gttattgacc tgctgtacca acaccgtgtt
     1441 gatccggatg ttcctattga agatgttgca ggaacggtga aagacctcat cgcagaaggt
     1501 aaggtcaaac atttcggtct gtccgaggct ggcgcacaaa ccattcgtcg tgcgcatgcg
     1561 gtacaacctg tcaccgccct gcaaagcgaa tattccatgt ggtggcgtga gcctgaacag
     1621 gaaattctgc cgttgctgga ggaactgggg attggttttg tccccttcag cccactaggt
     1681 aaaggtttcc tgaccgggtc gattaagccg ggaaccactt tcggcaaaga tgattaccgc
     1741 agcaccgtac cgcgtttcgc agcacaagcc attgaagcta atgaaaaact ggtcaccttg
     1801 ttaggtgaac tggctgcgga gaaaggggta acgtctgcac aaatcgcact ggcatggctg
     1861 ttagcacaaa agccgtggat tgttcccatc cctggcacca ctcaactgca ccggctggaa
     1921 gaaaacctgg ccgctgctga cattgttctt tcgcagaaag atacgcagca gataaccgag
     1981 gcgctggaaa caattaaaat cgtcggtgaa cgttactcgc ctgaacacca ggcacgcgta
     2041 ggccgctaat acccaaacgg gtccaggaac aaaggacccg ttattcggtg tagcgaagcg
     2101 catcgattaa cagcgcaaaa gcaggcggat gctgcttgcg gctggggtag taaagataat
     2161 atccgggaaa agttggacac cactcctgta acacctgaat aagcttgcct gactttatat
     2221 actcctgaac catgtcttca gggacacaag cgatcccaaa ccctgataac gcggcatcaa
     2281 ttctttctgg caatggacta cacgtcagct gcccatcaac cttaacgcga agagttttcc
     2341 cttctcgctc gaactcccag tggtaaagtc caccggcagt tggcaaacgc atgttgatgc
     2401 agcgatggtt ttgtagctcg tgaggcgttt caggcgcagg gtttgcagca aaatacgccg
     2461 gtgcccccac cacagccatc cgcatatctg gtccaattct gaccgcaacc atatctttat
     2521 caacattttc acccagtcgt atcccggcat caaaacggcc ttcaacaata tcgacaaaac
     2581 cgttatccac caccagttcg acactgattt cgggatactt tctgagaaaa ggttttagct
     2641 tcggccagac cagacttcgc gcggcatgtt ccccggcaga taaacgaata ttcccggagg
     2701 gcgtgccgtt ctgttgaata agtgactcca gttcctgttc aagctcgtca atacgcggtt
     2761 caaggcaggc aatgattctc tcccccgctt ctgtgggagc cacactgcgg gtagtccgtg
     2821 tcaggaggcg gatattgagc ctttcctcca gagccttcat tgcatggcta agtgcagact
     2881 gagagacgcc gagtttgccc gctgctttgg taaaacttcg ctctcttgcc accacaagaa
     2941 agatttgcag ttcgttgaag ttttctttga gcattggcga tttccttgta gaacatcctc
     3001 ttcacagaca ctcatgaagg ggggggcata gatgctatta ttttagccat attattagta
     3061 acaatgataa tctggctgta tcgaaaataa ttctaaactt tcaggagatt tattatgaat
     3121 aaacgtatcc tcacgattgt gccattcttc ttaccgatat tctgacacat atcatcaatt
     3181 ttcgagtttt actcttttct ccaccataaa atcaataaaa gcacgaactt tagagggcat
     3241 tctcacccgt gatgaaacat aaatataaaa tccaggaaat gaaacagccc aatcctcaag
     3301 aatcggctgt aacttgcccg cattgatatg ttcagcgatt gcaaaatcca gatgttgaat
     3361 gatacccgtt ccattcagcg ccattcgtat catgctttcg tcatcattag aaatatactt
     3421 acctgaaacc tctatttcga actcttcatt accccccaca acattagaaa actcccatgc
     3481 agagagtgca ccgctacttt tatgccgcaa ccgaaggcaa ttatggttac tcagttcctg
     3541 aggcgtttca ggaataccgt gttcttttaa ataatctggc gatgccacag taaccagttt
     3601 aataagcgga gaaacaggaa cggcagtcat atgctcatcc agtccttgct caagacgtat
     3661 gcccacatca cagccgtccg caataatgtt agataaaccg tcatccataa tgagttctat
     3721 ctttgttttg gggtaacgag taagaaactc gccaatatga ggctcaacaa gcatccgtgc
     3781 tgcaacacgg gaggaattta ttctcaacag gcctgtaggt tcaagttggg tatcaccaac
     3841 atctttcaaa gcatcatcaa tttgccccaa agccgacacc agcacttcat acagacgctg
     3901 tccttcctca gtaagcgaca tattgcgggt cgtacggtat atcagcttgg tgttaagacg
     3961 ttcctcaaga gattttaaat tctgggacaa tgcggcccgc gtaataccca tttcagaagc
     4021 ggctctggaa aaactgccgg cacgaacaat aagcgcaaac caggcaagag aaggcaaaat
     4081 tgaaggctct attttcatca ttgtaaacct taacttaaca ctgtgataga tattagacca
     4141 ttgatgataa ccaatacaag tcatactatt acgccgttct cgatatgaac acgaatgcca
     4201 gttgcggata atagaaatga tatttaaatc tgtttaggca aatccccgga gcttgaaatg
     4261 aaaactgttt cgataaaaca cacttactgg gatatagctg ctgatatcta tttccctgac
     4321 gattttaata gtgaaaaaaa gtatcctgcc attatcagcg cccatccaat tggaagctgc
     4381 aaagaacaga catcgggtag tgtgtatggt gccgccctgg caaaagctgg ctttatcgtc
     4441 attgcttttg atgccagttt tcagggaagc agcggtgggg aaccgcgtta tctggaagat
     4501 ccaacaatgc gagtaaaaga cttcagcatt gtcgttgatt accttaccag cttaccttac
     4561 gttgatgccg ggcgtattgg tgtactgggt atttgtggtg gaggtggata tgccattaat
     4621 gccgcaatga cagaacgtcg aattaaagct attggtactg tcacgggtgc aaactacggg
     4681 cgtcttatgc gtgagggatt tacagcgttt aatcctatcg gagcactcga agcaatggcg
     4741 caacaaagaa cagacgaagc taacggcgca aaacttcgcg tagatgattt actcccctct
     4801 tctccggatg cagctcttga agcgggatta acagaaatag acctttttga agccacagaa
     4861 tattatcgtt cgccacgagg ctgtgcacct aatggggtaa atcgttcgct attctcacat
     4921 cagactgttg ccgtgggttg ggatgcattt catcttgcag aagtgctgtt aactcaacca
     4981 ataatggttg tggtgggcga tagagtgggc gcatttggtg cctaccgtga cggctgcgaa
     5041 attattggcc gcgctgcctc aaaacataaa gagctggtcg tggttgaagg ttattcacat
     5101 tacgacctgt atgataagcc agaacctgtg aaacaggcac tcgataaatt aattcctttt
     5161 tataaaacac atttgtaata atcttacagg gagatccctt atctccctga catttatcct
     5221 ttattgacta cacaacaata ataaaattac tcaaaaagaa atttcattaa aaaacaaaaa
     5281 agcagcaact caattattaa gattattaga aaccctttaa agggattata aataaagcca
     5341 gaactcttgg ttccaaagct ggcaacaact aaccagttaa aacatacgca tcaaaatcag
     5401 ataaacattt gaggtattgt tatgacaaat aaacacccat cattgttttc tccttttatg
     5461 ctgacagaaa aaaataaagc tacgtaatcg tatcgttatg gctcctatga cgacctggtc
     5521 agcgaatcct gatggaacca tctctgagca agaactcgaa ttttataaac gtcgctcaca
     5581 aaatgttggt ttagtaataa caggatgtac ctatgtcacg ccaagcggca ttgggtttac
     5641 tcatgaattt gccgcgtatg acgaccgttt cattaacagc cttgaaaagt tagcagcggc
     5701 agccaaaagt gggggtgctc cggctatttt gcaggttttc cacgccggta ataaagccat
     5761 cccggagctt gtaccaaata acgatgttat tagtgccagc gcatcaagcg taaaatctgg
     5821 tgactttatg aagagagtgg tacaaagcag ggaaatgact gaaaacgaaa ttcaggaaac
     5881 cattagagca ttcggagatg tcacaaaaag ggcgattaaa gcaggttttg atggcattga
     5941 acttcatggt gcgcacggat tccttctaca gaatttcttc tcccctctat ttaaccagcg
     6001 taatgaccga tggggaggag atctcgaagg acgtatgcgc ttcccgctgg ccgtattaca
     6061 ggaagtaaaa aatgtagtct atgaatatgc gacaaagccc tttgccatcg gttacagaat
     6121 atctcctgaa gaatctgcaa caggggggct gcggatagag gatacttata aactccttga
     6181 tcggctcatt tcatccggta tttcgtatat acatacctcg ctggtcagta ttaacgatag
     6241 ctaccctgtt gagtcgccaa atggcccccg aactattgaa ttgattttaa atcatattgc
     6301 aggcagagtg ccggtaatcg ctgcaggtaa aatacgcaca ccgtctcagg ctcaagaagc
     6361 aatttccact ggcctgccac ttgttgcaat cggaaaaggg ctggtgataa atccagaatg
     6421 ggtgacttta gctgaatcag gtagaggcca tgaaattcaa acaaccttta atccacaact
     6481 tgttcctgaa ttaaccattc cggataaact atgggatcaa attcaggcat cgaaaggaac
     6541 tggctggttt ccgttaatgg attaaatatc tattactctc ccgagagtaa aactatcaaa
     6601 tcaatgagta accgatacag cctcacaaat tctttcagat aattaacccg tcactgcggc
     6661 acgttctctc ataaggcgtg cctgcagtgt aatccagtat gtatccggca tagttgacgg
     6721 tttaatagag ctcaattcct cgcccaggtt accaacaacg atatcttgta taacacccca
     6781 ggagatacga cattatgatg agatgcgata tgctcctcag cgccaatcaa cgccaggact
     6841 gggccagtct tgagatcaga ctcattcccg gaactgactt caggcgacat atatcaatga
     6901 cgttggtgca ataaaagata gccgatgtaa tacatctatt caaatttgtg atacatgtca
     6961 aaaaaattgg ttgaccaaac tcgttattta tataagggca cttacgaagt gcgctctttt
     7021 ttaaagcgag gaagtaccaa tgaaagagaa taaagtacaa caaatcagtc ataaactgat
     7081 taatatcgtt gtttttgtcg caattgtaga atacgcctat t
//
LOCUS       DAZUCE010000245         7079 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZUCE010000245 DAZUCE010000000
VERSION     DAZUCE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.245"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            32..700
                     /gene="ecpB"
                     /locus_tag="ACHY03_004382"
                     /gene_synonym="yagY"
     CDS             32..700
                     /gene="ecpB"
                     /locus_tag="ACHY03_004382"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFS3907539.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            726..3251
                     /gene="ecpC"
                     /locus_tag="ACHY03_004383"
                     /gene_synonym="yagX"
     CDS             726..3251
                     /gene="ecpC"
                     /locus_tag="ACHY03_004383"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFS3907540.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            3241..4884
                     /gene="ecpD"
                     /locus_tag="ACHY03_004384"
     CDS             3241..4884
                     /gene="ecpD"
                     /locus_tag="ACHY03_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFS3907541.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            4853..5563
                     /gene="ecpE"
                     /locus_tag="ACHY03_004385"
     CDS             4853..5563
                     /gene="ecpE"
                     /locus_tag="ACHY03_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFS3907542.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            5876..6205
                     /gene="ykgJ"
                     /locus_tag="ACHY03_004386"
     CDS             5876..6205
                     /gene="ykgJ"
                     /locus_tag="ACHY03_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFS3907543.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(6453..7067)
                     /gene="yagU"
                     /locus_tag="ACHY03_004387"
     CDS             complement(6453..7067)
                     /gene="yagU"
                     /locus_tag="ACHY03_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFS3907544.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
BASE COUNT         1817 a         1855 c         1828 g         1579 t
ORIGIN      
        1 cccccctgct ttattcctgg acggaattgt tatgaaaaag caccttctgc ttctcgctct
       61 gctgttatcc ggaatatctc cggcccaggc gctggatgtc ggcgatatat catcgtttat
      121 gaacagtgac agcagcacgc tgagcaaaac gatcaaaaac agtaccgaca gtggtcgcct
      181 tatcaatatc cgtctcgaac ggctctcttc accgcttgac gacgggcagg ttatctcaat
      241 ggacaagccg gacgagttgc tactcactcc cgccagcttg ctgctacccg cccaagccag
      301 cgaagtgatc cgcttcttct ataagggacc cgcagatgaa aaagagcgct actaccgcat
      361 tgtctggttt gatcaggccc tcagtgatgc gcagcgcgat aatgccaacc gcagcgctgt
      421 ggccactgct tccgcccgca tcggcaccat tctggtcgtc gcccctcgcc aggcgaacta
      481 ccactttcag tacgccaacg gctccctgac aaatacagga aacgcgacgc tgcggatcct
      541 cgcctacggc ccttgcctga aagccgccaa cggtaaggag tgtaaagaga attactacct
      601 gatgccgggc aagtcacgtc gttttacccg cgtggacact gcggataaca aaggacgggt
      661 tgcactttgg cagggtgata agttcattcc cgtgaaatag atagctgtgc agatggataa
      721 cgacaatgcc tttacgacgg ttctccccag gactgaaagc ccagtttgcc ttcggcatgg
      781 tctttttgtt cgttcagccc gatgccagcg ctgctgacat aagtgcgcag caaataggtg
      841 gggtgattat tccgcaggcc ttcagtcagg cgcttcagga cggcatgagc gtcccgctct
      901 atattcatct cgccggtagc cagggtcgcc aggacgatca gcgaatcggc agcgctttta
      961 tctggctgga tgatggacag ctacgcatcc ggaaaataca gctggaagag agtgaagata
     1021 acgccagtgt cagcgaacaa actcgacagc agctgatggc tctggcgaac gccccgttca
     1081 atgaggccct taccatcccc ctgactgaca acgcgcagct ggatctcagc ttgcgccaac
     1141 tgctgctgca gctggtggtc aagcgcgaag cgctgggcac cgtactacgc tcacgtagcg
     1201 aagacatcgg gcagtccagt gttaacaccc tcagcagtaa tctgagctat aacttgggcg
     1261 tctataacaa ccagttgcgt aacggcggga gcaacacatc cagctatctg tcgctgaata
     1321 acgttactgc gctgcgcgaa catcatgtgg tgctcgacgg ctcgctgtac gggatcggta
     1381 gcggtcaaca ggacagtgaa ttatataaag cgatgtatga acgcgatttt gctggtcacc
     1441 gatttgccgg tggaatgctc gacacctgga acttgcagtc cttagggccg atgaccgcca
     1501 tttcagcagg gaagatttac ggcctttcct ggggaaacca ggccagctcc accatcttcg
     1561 acagcagcca gtcagccacg ccagtgatcg cctttttacc ggcggcgggc gaagtacatc
     1621 tcacccgtga tgggcggcta ctaagcgttc agaacttcac tatgggcaat catgaagtgg
     1681 atacccgggg tctaccgtac ggtatttacg atgtggaagt tgaggtgatc gttaacggtc
     1741 gcgtgatcag caaacgtacc cagcgggtca ataagctgtt tagccggggg cgcggcgtcg
     1801 gtgcaccact ggcgtggcag gtatggggcg gtagctttca tatggatcgc tggtcggaaa
     1861 acgggaaaaa gacgcgacca gctaaagaga gttggctagc aggtgcttcg acctccggct
     1921 cactgagtac gcttagctgg gcggcaacgg gatatggata cgataatcag gcggtgggtg
     1981 aaacccgtct gacgctgccg cttggggggg caatcaacgt taacctgcaa aatatgctgg
     2041 ccagtgacag ctcatggagc agcatcggca gcatcagcgc cactctaccg ggaggcttta
     2101 gttcgctgtg ggttaaccag gaaaaaaccc gcattggcaa tcaattgcga cgtagcgatg
     2161 ccgacaaccg tgcaatcggc ggcacactca acctgaactc actgtggtcg aagctgggca
     2221 cattcagcat cagctacaat gatgaccgcc gttacaacag ccattattac acggcagatt
     2281 actatcaaaa tgtctacagc ggtccctttg gttcgcttgg cctgcgggcc ggtattcagc
     2341 gctataacaa cggcgacagc aacgccaata cagggaaata tatcgctctc gatctctcgc
     2401 taccactggg caactggttt agcgcaggga tgactcatca aaacggctac accatggcaa
     2461 acctgtcagc acgcaagcag tttgatgaag ggaccattcg cactgttggt gccaatctgt
     2521 cacgagccat ctccggcgat accggtgatg acaaaactct cagtggtggg gcgtatgcac
     2581 agttcgacgc tcgctacgcc agcggaacgc tgaacgtcaa tagcgcggcg gacggctacg
     2641 tcaataccaa tttaaccgcc aatggcagcg tcggctggca gggtaaaaac attgctgcca
     2701 gcgggcggac tgatggcaac gctggggtga tattcaacac cgggctggag gacgacggtc
     2761 agatcagcgc caaaatcaac gggcggattt tcccgcttaa cggcaagcgt aactatctcc
     2821 cgctctctcc ctatggaaga tatgaggtgg agttacagaa cagcaaaaac tcactcgaca
     2881 gttacgatat cgtcagcggt cgcaaaagtc atctgactct ctatccaggc aatgtcgctg
     2941 tcattgagcc agaggtgaag cagatggtta ccgtctccgg tcgtatccgt gcggaagacg
     3001 gcacactgct ggctaacgca cggattaaca accatatcgg ccgaacccga accgatgaaa
     3061 acggcgagtt tgtcatggac gtggataaga aataccccac tatcgatttt cgctacagtg
     3121 gcaataaaac ctgcgaagtg gcactggaac tcaaccaggc gcgcggtgcc gtctgggtcg
     3181 gtgatgtggt ctgcagcggc ctctcatcgt gggcggcggt gacgcagaca ggagaagaga
     3241 atgagagtta acctactgat agcgatgata atctttgcgc taatctggcc tgcaaccgcg
     3301 ctcagagcgg cagtgagcaa aacaacctgg gcggatgcac cggcacgcga gtttgtgttt
     3361 gtcgaaaaca actcagacga caactttttc gtcactcctg gcggggcgct ggatccgcgc
     3421 ctgaccggtg ccaaccgctg gaccggttta aaatacaatg gttcaggaac catctatcag
     3481 caaagcctcg gctacattga taacggttac aacaccggcc tttataccaa ctggaagttt
     3541 gatatgtggc tggaaaattc accagtttca tctcctttaa ctggcttgcg ctgcatcaac
     3601 tggtacgctg ggtgtaatat gaccaccagt cttatcctgc cgcaaaccac cgacgccagt
     3661 ggattttatg gcgcgacggt gaccagcggc ggcgcgaagt ggatgcacgg catgttgtca
     3721 gacgcgtttt accagtatct gcaacaaatg cccgtcggca gcagctttac aatgaccatc
     3781 aatgcctgcc agacctctgt gaactatgac gccagcagcg gcgcacgctg taaggatcag
     3841 gcctccggca actggtatgt tcgcaacgtc acccatacga aagcagcaaa tctacggttg
     3901 ataaataccc actcgctggc ggaagtattt atcaacagcg acggagtacc gactctgggc
     3961 gaagggaacg ccgactgccg gacgcaaacc atcggcagcc gttcaggatt aagttgtaag
     4021 atggttaact ataccctgca aacaaacgga ctcagcaaca cctcaatcca tatattcccg
     4081 gcgatcgcca actcgtcgtt agcctcggcc gtcggggcgt acgatatgca gttcagtctg
     4141 aatggcagtt catggaaacc ggtgagcaat actgcctatt actacacctt caacgagatg
     4201 aagagcgcag actcgatcta tgttttcttc tcgagcaact tctttaagca gatggtgaac
     4261 ctcgggatca gcgatatcaa caccaaagat ctattcaact ttcgctttca gaacaccaca
     4321 tcaccggagt ctggctggta tgaattttct acctccaaca cgctgattat caaaccccgt
     4381 gatttcagca tcagtattat ctccgatgaa tatactcaga caccgtcgcg ggagggatat
     4441 gttggcagcg gcgagtcggc actcgatttc ggctatatcg taaccaccag cggtaaaaca
     4501 gctgccgacg aagtgctgat caaggtgacc ggacccgcgc aggtgattgg cgggcgctcc
     4561 tattgtgtct tcagctccga tgacggtaag gcgaaagtac cgttcccggc gacgctttcc
     4621 tttattaccc gcaacggagc tacaaaaacc tacgatgccg ggtgcgatga tagctggcgg
     4681 gatatgaccg atgcgctgtg gttgaccaca ccgtggactg atatctctgg cgaagtgggg
     4741 cagatggata agaccacagt caaattttcg attccaatgg ataacgccat ttcgctgcgt
     4801 acggtagatg ataacggctg gtttggcgaa gtcagcgctt caggagagat tcatgttcag
     4861 gcgacgtggc gtaacattaa ctaaggccct gctgacagtg gtctgtatgc tggcggcacc
     4921 tttaacacag gcgatttcgg tcggcaatct gacattttcg ctgccgtccg agactgactt
     4981 tgtcagcaaa cgtgtagtga ataacaacaa aagcgcacgg atataccgta ttgccatcag
     5041 tgctattgat agcccgggca gcagtgaatt gcgcacccga ccggtggatg gtgaactgct
     5101 tttcgccccc cgccagctgg cgttgcaggc tggtgagagc gagtatttta aattttacta
     5161 tcatggtcca caggataacc gcgagcgcta ctaccgggtc tcatttcgcg aggtccccac
     5221 tcgtaacctg acaaaacgca gccctaccgg cggtgaggtc agcacggagc cggtggtggt
     5281 gatggatacc attctggtag tacgaccacg tcaggttcag tttaaatggt ccttcgacaa
     5341 ggtgacagga acggtaagta acactggcaa cacatggttt aagctactga ttaagccagg
     5401 atgtgattcg accgaagagg aaggcgatgc ctggtatcta cgtccgggag acgtggttca
     5461 tcagcctgag ttacgtcagc cggggaatca ttatctggtc tataacgaca aattcattaa
     5521 gattagcgat tcctgtccgg ctaagccccc ttcggcggac tgatacttat ccatcggtcg
     5581 agggaagaat tccacctcag agactcaaaa aacgtttaaa tgatgtggaa gcgatcgtca
     5641 atgtcatctg taacgatggg ctttctcata tgcacaccat agtgttcagg agacacagaa
     5701 aggtatcagg ctgcctgcta acgagtggtt tattcacgaa atagtacgta aaatcggtaa
     5761 cggctggaaa tcattcaata ctcacactat cgaaagtaca ccagccaacc gcggtacgtt
     5821 cttacatacg atgtaccgct gttctcttta cgatttatag ctgtactggt gaattatgag
     5881 caatctgaat ccatgcatga cgtgtggtgc ctgttgtgca tttttccgcg tctcttttta
     5941 ctgggccgaa gccgacgatg ctggcggtac cattcccgcc aggctcactg aacaaatatc
     6001 cccttttcac cgatgcatga gcggtaccaa tcagaaaaac ccccgatgta ttgcccttgc
     6061 aggaaccccg ggcaaaaatg cctgctgcac gatatataaa aatcgatcgt ccacatgcag
     6121 agaattcgcc atgtctggtg agaacggagt cgtcaatgag gcttgcaatc gtgcaagggc
     6181 taaatacggg ctgacaccgt tataaacata caataattaa ttgcactgcc ccgccagcga
     6241 taatagcggg gcttcgtttt tcaggggtaa caaaacccga aacttcttgt atttgccagc
     6301 aacaatgcct ctcttctgta gcgttcttcg gatctacatc atcctgaagt agcgcgatat
     6361 actcactgac tatctgaatc agaatataaa aagcaatgtt tttaacctat aaaaatggcg
     6421 ctgtatttgc gccattttta tcattcaatg cattatctgt ttgagcctaa agggatctca
     6481 gggtctggct catgagtaat tctgtttcgt aaatctctgc gaataatttc aatggaccag
     6541 aaccagacta aatgtccaaa aatttcagaa acattctcat accacgggag atcaaacaga
     6601 ggtggtgtca gtcccatgag agggaatgaa atcatatgaa caaaaagttg ggctaaagca
     6661 cctgccagta agccctgcca gagtttaatt tttggaaata cttcagcgac cacacaataa
     6721 ccgacagcaa acactatcga aaagataatg tgcgtaacac caacccagtt aaagacatgc
     6781 ccggcaaagg tataaacagc cgcattggga tctgtcagcc ccagccagtc tcgaagaaaa
     6841 atatacggag gattgagaaa attacgcgag caatcaattt gcccagcagc cctgattaag
     6901 gattccgggc cacacgctgc attaaacata tccaccgggc tacgtggcgg caatggaact
     6961 tcagcccccc acttcacgaa tgcggaaaca acgccagcaa tcagcccaat gaatgcagca
     7021 agaccataac gtctgcggtt cggtggagtt tgttcaaata tattcatatc taccctgct
//
LOCUS       DAZUCE010000246         7071 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZUCE010000246 DAZUCE010000000
VERSION     DAZUCE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.246"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..2021
                     /locus_tag="ACHY03_004388"
     CDS             <1..2021
                     /locus_tag="ACHY03_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS3907545.1"
                     /translation="AYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSA
                     ERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGT
                     AQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVP
                     VLQRAGYTRYALAMGEYRSGNNLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFN
                     LGIGKDLGLFGAFSFDITQANTTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYS
                     TQGFYNLSDSAYSRMSGYTVKPPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNY
                     GTTFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTL
                     NVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNT
                     HGGNTSSGTSGYSSLNYRGAYGNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPL
                     GDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRVALNANSLADNVELD
                     ETVVTVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENG
                     QVYLTGLPQSGKLQVSWGNDKNSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            2035..2565
                     /gene="ydeS"
                     /locus_tag="ACHY03_004389"
     CDS             2035..2565
                     /gene="ydeS"
                     /locus_tag="ACHY03_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3907546.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSTRQFPTTGSTSPALPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            2578..3081
                     /gene="ydeR"
                     /locus_tag="ACHY03_004390"
     CDS             2578..3081
                     /gene="ydeR"
                     /locus_tag="ACHY03_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3907547.1"
                     /translation="MKRLHKSFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            3141..4055
                     /gene="ydeQ"
                     /locus_tag="ACHY03_004391"
     CDS             3141..4055
                     /gene="ydeQ"
                     /locus_tag="ACHY03_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3907548.1"
                     /translation="MGKAISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            4389..6668
                     /gene="ydeP"
                     /locus_tag="ACHY03_004392"
     CDS             4389..6668
                     /gene="ydeP"
                     /locus_tag="ACHY03_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFS3907549.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLMEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTQSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            6916..>7071
                     /gene="safA"
                     /locus_tag="ACHY03_004393"
     CDS             6916..>7071
                     /gene="safA"
                     /locus_tag="ACHY03_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFS3907550.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIA"
BASE COUNT         1976 a         1564 c         1652 g         1879 t
ORIGIN      
        1 atgcatattt gaatttacag agtggcatca acattggtag ttggcgactg cgcgataact
       61 caacgtggag ttataacagt gggagtagca attcatctga cagcaataaa tggcagcata
      121 tcaatacgtc ggctgaacgt gacattattc ccttacgctc acggttaacg gtaggtgata
      181 gttataccga tggcgatatt tttgatagtg tgaactttcg tggcctcaaa ataaattcaa
      241 cagaagcgat gttgcccgat agccaacatg gtttcgctcc ggtgattcat ggtattgccc
      301 gtggcaccgc acaagtgagt gtaaaacaga acggatacga tgtttatcag actactgttc
      361 cacccggccc ttttactatt gatgatatca actctgcggc caatggtggt gatctgcaag
      421 taaccataaa agaggcagac ggcagtattc agacattata tgttccttat tcgtctgttc
      481 cggttctcca acgtgctgga tatacgcgtt atgcgcttgc catgggggaa tatcgtagtg
      541 gaaataacct gcaaagctcc cccaggttca tacaaggtag cttgatgcat ggactggaag
      601 gaaactggac accttatggc ggaatgcaaa ttgcagaaga ttatcaggcc ttcaaccttg
      661 gtattggtaa agatttagga ctttttggtg ccttttcttt cgatatcacg caggccaata
      721 cgacacttgc agatggcacc cgtcacagcg ggcaatcggt taaatccgtc tacagcaaat
      781 ccttctacca gacaggaacc aatatccagg tcgcaggata tcgctattct acgcaaggtt
      841 tttataactt atccgacagt gcctacagtc gaatgagtgg ttacaccgtc aagcctccta
      901 ccggagacag caatgagcag acacaattta ttgattattt taatctgttc tacagtaagc
      961 gtggtcagga acaaataagc atctctcagc agcttggaaa ttacggtacg acatttttca
     1021 gtgccagtcg ccaaagttac tggaacacgt cacgcagcga ccagcaaata tcatttggat
     1081 taaatgttcc gtttggtgat attacgactt cactgaatta cagctattcc aataatatat
     1141 ggcaaaacga tcgggatcat ttactcgctt ttacgcttaa tgttcccttc agtcattgga
     1201 tgcgtacaga cagtcagtcg gcatttcgta attcaaacgc cagttacagt atgtcaaacg
     1261 atttgaaagg cggcatgacc aatctatcgg gggtttatgg cactctgctg ccggataata
     1321 acctgaatta tagcgttcag gtcggtaaca cccacggagg taatacatcg tctggcacca
     1381 gtggttacag ttctcttaat tatcgtggag cttacggtaa tacgaatgtc ggttacagtc
     1441 ggagtggtga cagcagccag atctattacg gaatgagtgg tgggattatt gctcatgctg
     1501 atggcatcac ctttggacag ccgctgggcg acacaatggt tctggttaag gctcctggcg
     1561 ctgataatgt caaaatagag aaccagaccg gaattcatac cgactggcgt ggctatgcca
     1621 tattaccatt tgcgacagaa tatagagaaa atcgtgtcgc tcttaacgcg aattcccttg
     1681 cagataatgt tgaactggat gaaaccgtgg tcactgtcat cccaactcac ggtgctattg
     1741 ccagagcaac atttaatgca caaatcggcg ggaaagtatt aatgacgttg aagtacggta
     1801 ataaaagcgt tccattcggt gcaattgtca ctcacggaga gaataaaaat ggcagcattg
     1861 tcgcggaaaa tggtcaggtt tatctgactg gacttccaca gtcagggaaa ttacaggttt
     1921 catggggcaa tgataaaaac tcaaactgta ttgtcgatta caagcttcct gaagtctctc
     1981 ctggaacctt gctgaaccag cagacagcaa tctgtcgcta aggaacaata gattatgaaa
     2041 tacaataacg ttattttcct cggtttatgt ctggggttaa ccacctattc tgctttatcc
     2101 gcagatagcg ttattaaaat tagcgggcgc gtcctcgatt atggctgcac agtctcatcg
     2161 gattcgctta attttaccgt agatctccaa aaaaacagta ccagacaatt tccaacgacc
     2221 ggtagcacaa gtccagccct cccttttcag attacgttaa gtgaatgcag caaagggaca
     2281 acgggggttc gggttgcatt taacggtatt gaggatgcag aaaataatac tctgttgaaa
     2341 ctggatgagg gaagcaatac ggcctccggt ttaggtatag aaatactgga cggaaatatg
     2401 cgtccggtga aactgaatga ccttcatgcc gggatgcagt ggatcccact ggtaccagaa
     2461 cagaacaata ttttgcctta ctccgctcgt cttaagtcaa ctcagaagtc cgtcaatccg
     2521 ggactggtga gggcttcggc aacctttacc cttgaatttc aataatcaga gtcagatatg
     2581 aaaagacttc acaagagctt cctgttagct acgttttgcg cgttattcac agcaactctc
     2641 caggccgccg atgtcactat cactgttaat ggtcgggtag tcgctaaacc ctgcactatt
     2701 caaaccaaag aagctaacgt taatctcggg gatctttata cgcgcaatct gcaacaacct
     2761 ggttctgcat ctggctggca caatattact ttgtcattaa ccgattgtcc ggttgaaaca
     2821 agtgcagtga cggcaatcgt gacaggttca actgacaata cgggttatta caaaaatgaa
     2881 ggtactgccg aaaatattca gatagagctg agggatgacc aggatgctgc gttaaaaaac
     2941 ggcgatagca aaacggttat tgttgatgag atcactcgta atgcacagtt tccacttaag
     3001 gcaagagcta tcacggtgaa tggaaacgca agccagggaa cgatcgaggc gctaatcaat
     3061 gtgatctaca cctggcaata acgtcgattc gattttctta gccttctttt taaatcaatt
     3121 ttttaacctt aggatatatc atgggtaaag caatcagtat aaaggtcctg ttcggtattt
     3181 atcttctatt aatggcgggc aaagtttttg ccttcagttg taatgtcgat ggcgggtcaa
     3241 gtattggagc aggtacaacc tctgtttatg ttaatctcga ccctgtaata caaccgggac
     3301 agaatctggt tgtagacttg tctcagcata taagttgctg gaatgattac ggcggctggt
     3361 acgacactga tcatataaac ctggtacaag gctcagcttt cgcaggctct ctacagtcat
     3421 ataaaggatc gctgtactgg aataatgtga cctacccctt cccgttgacg acaaatacca
     3481 atgtattgga tattggtgat aagacaccga tgccattacc gttaaaactc tatattaccc
     3541 cagtcggggc ggccggtgga gttgtcataa aagccgggga agttattgcc cgtattcata
     3601 tgtataaaat tgccacgtta ggaagcggaa atccgcgtaa ttttacgtgg aatattattt
     3661 caaataacag tgttgttatg ccaacaggcg gctgcactgt tgatagtcgt aatgtcaccg
     3721 tgaatctccc ggactttcct ggaagcgcag aaatccccct tggcgtatat tgctcaagtg
     3781 aacaaaagct ctcattttat ctttcgggtg ctaccactga cagtgctaga caagtgtttg
     3841 ctaacacagc accagatgca acaaaagcat cgggagtggg tgtctctctg atgcgtaatg
     3901 gaaaaatact ggctacggga gaaaatgtct ccctggggac agtaaataaa agcaaagtac
     3961 ctctgggttt aagcgcaacc tatggacaaa cagggaacaa agtttcagcc ggaaccgtcc
     4021 agtcagtcat tggtgtcact tttatctatg aataattgtg attttgacct tagtctatgc
     4081 gctccattca cgaaaattgc gcggataatt attttgtgaa ggctattatc ctacacctgt
     4141 aagaaaatcc gcgcacaatt gaagccagag tcttattttt tatattgaaa aacatgttaa
     4201 tttaaacact atgagagatg atgcttacct tatcgtattt aatctattgt ttaacgataa
     4261 tagattaaaa atagaaagag agtagtcccc tactcttata tatccatgtt ggcgataatc
     4321 cctttgtatg tactgtgcat catcgctatt acaaatccta ataattcatt tccacacagg
     4381 ataagtagat gaagaaaaaa attgaatcct accagggagc tgccggtggt tggggtgctg
     4441 ttaaatccgt agcgaatgca gtacgtaagc agatggatat acgccaggat gttattgcca
     4501 tgtttgacat gaataagcca gagggctttg actgtccggg ttgtgcatgg ccagatccta
     4561 agcacagtgc gtcattcgac atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca
     4621 cggataagca ggtaaacgcc tctttctttg ctgagaatac ggttcaatca ttacttacct
     4681 ggggagacca cgagcttgag gctgcggggc gactcactca gcctttgaaa tatgatgccg
     4741 tcagcgactg ttacaagcca ttaagctggc aacaagcttt cgatgaaatt ggcgcacgcc
     4801 ttcaaagcta tagtgatccc aatcaggttg aattctatac ttcgggccgc acttccaatg
     4861 aagctgcctt tctttatcag ctttttgccc gtgaatacgg gagcaataac tttcccgact
     4921 gctccaacat gtgccatgaa ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta
     4981 aagggaccgt gttgctggaa gactttgaga agtgcgattt agtcatttgc attgggcata
     5041 accctggtac aaaccaccct cgcatgctga cttcgttgcg cgctttagtg aaacggggag
     5101 cgaaaatgat cgccatcaat cctctacagg aacgtggcct ggagcgattt accgcaccgc
     5161 aaaacccgtt tgaaatgctg acgaactctg agactcagtt ggccagtgcc tactacaacg
     5221 tgcgcattgg tggcgatatg gcgttgctca aggggatgat gcgcctgtta attgagcgcg
     5281 atgatgctgc aagtgccgca ggtcggccct cattgcttga tgacgaattt attcaaacgc
     5341 ataccgtcgg ctttgacgag ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg
     5401 aacgtatttc tggactaagt cagacacaaa tcgccgaact ggctgatgca tatgccgctg
     5461 ccgaacgcac cattatctgt tacggaatgg ggatcactca gcacgaacat ggtacccaga
     5521 atgtacagca actggtcaat ctgctgttga tgaaaggtaa cattggcaag cctggtgcgg
     5581 gtatctgccc actacgtggc cactctaatg tacagggcga ccgaaccgtc ggtatcaccg
     5641 agaaaccgtc tgcagagttt ctggctcgcc tgggtgagcg ctatggcttc acaccacctc
     5701 aagcacctgg acatgctgca attgccagca tgcaagcaat atgtacaggg caggctcgag
     5761 cattgatctg catggggggc aactttgcac tggcaatgcc agatcgggaa gcgagcgctg
     5821 taccgttaac gcaattagat ctggcggtac acgtagccac taagcttaac cgctctcatc
     5881 tgctgaccgc acggcatagc tatattctgc cggttctggg acgtagcgag attgacatgc
     5941 aaaaaagcgg tgcgcaggcg gtaaccgttg aggattcaat gtcgatgatt catgcctcgc
     6001 gtggcgtgtt aaaacccgcc ggtgtaatgc tgaaatcaga gtgtgcagtg gtcgcgggaa
     6061 tcgcacaggc agcactaccc cagagcgtgg tagcctggga gtatctgatg gaagattatg
     6121 atcgcattcg caatgacatt gaagctgtgc tgccagagtt cgccgactat aaccagcgca
     6181 tccgacatcc tggtggtttt cacctgataa acgctgctgc tgaaaggcgc tggatgactc
     6241 agtcaggtaa ggctaatttc attaccagca aagggctgtt agaagatccc tcttcagcgt
     6301 ttaacagtaa gctggtcatg gcgacagtac gcagccacga tcagtacaac acgacgattt
     6361 atggtatgga tgatcgctat cgaggggtat tcggtcaacg agatgtggtc tttatgagtg
     6421 ctaaacaagc taaaatttgc cgtgtaaaaa acggcgaaag agttaatctt attgcgctta
     6481 cgccagacgg taagcgcagc tcacgccgca tggatagatt aaaagtggtc atttacccta
     6541 tggctgaccg ctcactggtg acctattttc cagaatcgaa tcacatgcta acacttgata
     6601 accacgatcc attaagtggc attcctggct ataaaagtat tccggttgaa ttagaaccat
     6661 caaattaatg cctcttctca tttcttctgc tgtcatccgc acagcagaag aattcctcat
     6721 tgactattat cgcgcaattt gctcacatgg attaaattaa actacatact ataagatata
     6781 aacttctgcc tacagctgta agaaactccg ctcagtactg aagcaccagt cctatttcct
     6841 cttttctcca gcctgttata ttaagcatac tgattaacga tttttaacgt tatccgctaa
     6901 ataaacatat ttgaaatgca tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac
     6961 tataaagaaa tcatgtctgt aattgtggtt gtcttattac tgacccttac gttgatagcc
     7021 attttttcgg caattgatca gctgggcatt tcagaaatgg gtcgcattgc a
//
LOCUS       DAZUCE010000247         7035 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZUCE010000247 DAZUCE010000000
VERSION     DAZUCE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.247"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            24..1457
                     /gene="cscA"
                     /locus_tag="ACHY03_004394"
     CDS             24..1457
                     /gene="cscA"
                     /locus_tag="ACHY03_004394"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFS3907551.1"
                     /translation="MTQSRLHAAQNALAKLHERRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADSWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSILLPQGETLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(1465..2460)
                     /locus_tag="ACHY03_004395"
     CDS             complement(1465..2460)
                     /locus_tag="ACHY03_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS3907552.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGSVTRIPCPLLIRCST"
     gene            2685..2909
                     /locus_tag="ACHY03_004396"
                     /pseudo
     CDS             2685..2909
                     /locus_tag="ACHY03_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2927..4255
                     /gene="dsdA"
                     /locus_tag="ACHY03_004397"
     CDS             2927..4255
                     /gene="dsdA"
                     /locus_tag="ACHY03_004397"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFS3907553.1"
                     /translation="MENAKMNSLIAQYPLVKVLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(4363..5901)
                     /gene="emrY"
                     /locus_tag="ACHY03_004398"
     CDS             complement(4363..5901)
                     /gene="emrY"
                     /locus_tag="ACHY03_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFS3907554.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5901..>7035)
                     /gene="emrK"
                     /locus_tag="ACHY03_004399"
     CDS             complement(5901..>7035)
                     /gene="emrK"
                     /locus_tag="ACHY03_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFS3907555.1"
                     /translation="SNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMISTDDAYVTGNA
                     DPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLY
                     LQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAA
                     IQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGE
                     TVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGIN
                     MGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNED
                     IAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         1924 a         1682 c         1655 g         1774 t
ORIGIN      
        1 tttgcttaac caggatgata aaaatgacgc aatctcgatt gcatgcggcg caaaacgcac
       61 tagcaaaact tcacgagcgc cgaggtaaca ctttctatcc ccattttcac ctcgcgcctc
      121 ctgccgggtg gatgaacgat ccaaacggcc tgatctggtt taacgatcgt tatcacgcgt
      181 tttatcaaca tcacccaatg agtgaacact gggggccaat gcactgggga catgccacca
      241 gcgacgatat gatccactgg cagcatgagc ctattgcgct agcgccagga gacgataatg
      301 acaaagatgg gtgtttttca ggtagtgctg tcgatgacaa tggtgtcctc tcacttatct
      361 acaccggaca cgtctggctc gatggtgcag gtaatgacga tgcaattcgc gaagtacaat
      421 gtctggctac cagtcgggat ggtattcatt tcgagaaaca gggtgtgatc ctcactccac
      481 cagaaggaat catgcacttc cgcgatccta aagtgtggcg tgaagccgac tcatggtgga
      541 tggtagtcgg ggcgaaagac ccaggcaaca cggggcagat cctgctttat cgcggcagtt
      601 cattacgtga atggactttc gatcgcgtac tggcccacgc tgatgcgggt gaaagctata
      661 tgtgggaatg tccggacttt ttcagccttg gcgatcagca ttatctgatg ttttccccgc
      721 agggaatgaa tgccgaggga tacagttacc gaaatcgctt tcaaagtggc gtaatacccg
      781 gaatgtggtc gccaggacga ctttttgcac aatccgggca ttttactgaa cttgataacg
      841 ggcatgactt ttatgcacca caaagctttg tagcgaagga tggtcggcgt attgttatcg
      901 gctggatgga tatgtgggaa tcgccaatgc cctcaaaacg tgaaggctgg gcaggctgca
      961 tgacgctggc gcgcgagcta tcagagagca atggcaaact tctacaacgc ccggtacacg
     1021 aagctgagtc gttacgccag cagcatcaat ctatctctcc ccgcacaatc agcaataaat
     1081 atgttttgca ggaaaacgcg caagcagttg agattcagtt gcagtgggcg ctgaagaaca
     1141 gtgatgccga acattacgga ttacagctcg gcacaggaat gcggctgtat attgataacc
     1201 aatctgagcg acttgttttg tggcggtatt acccacacga gaatttagac ggctaccgta
     1261 gtattctcct cccgcagggt gagacgctcg ccctaaggat atttatcgat acatcatccg
     1321 tggaagtatt tattaacgac ggggaagcgg tgatgagtag tcgaatctat ccgcagccag
     1381 aagaacggga actgtcgctt tatgcctccc acggagtggc tgtggtgcaa catggagcac
     1441 tctggcaact gggttagcat aatatcaggt ggaacaacgg atcaacagcg ggcaagggat
     1501 ccgcgtcaca cttccccctt cacgaccttc aataatatgc aatgcagctt cccgcccgat
     1561 aatgtcatgt ggaagctgaa ttgtggtcag cggcggtaaa aacagatgcc cgacgccaac
     1621 cagattatca aagcccatta cggcgacatc ctgcgggata cgtaccccct tcgccaggag
     1681 aacctgataa gccacaaagg ctgcgcgatc gttaccacat atcagaacat caaaatctgg
     1741 tttgcccgat ttgaagtggt cattgagtaa acttgcgaga tcggtgtaat gatcatcacc
     1801 tgttgccatg tgaaattgtt tcacctcagc cagatctcgt ccagcatcac gccaggcctg
     1861 ctcaaatccc tgtcgacgat accctgttgc caacgcactt tccggtagcc agaagcataa
     1921 cggttgacga tagcccgccg cgagcaaatg ctgtgttgat tcatattgtg cagtgtaatc
     1981 atcagggata taactgggta acgctgggtc atccgccaca cagttcgcca atacaatatt
     2041 ttcaccatac agagactcag gtagcgtgat atgtcgcagc cccattgtag tatagataat
     2101 gccatccgga cggtgggcaa gcagctgacg tgccgcgcgg gcagcgtcat cttcagaaaa
     2161 aatattgatt aaaaaactat tccagccgaa ctcgctggcg gtttgctcaa tggcaagcag
     2221 aatatcaaca gagaaaggag tggtagccgt gtcctgcgcc agcacggcga gagtcgacgg
     2281 cttacgtcct tgagcgcgca tcttacgggc ggaaagatca ggaacataat tcagggtctg
     2341 gattgcctgc aatacgcggt cacgcgttgc aggacgcaca gattctgcat tatgcatcac
     2401 ccgggagact gtcatcatcg acactcccgc caggcgtgcg acatccttta atgaagccat
     2461 atccaagccg tttgccgtaa aacgggcact gtagcagaaa cagacgtcac tggcgagatc
     2521 caacgcccta tcacctgaca cggcaataca ataaaaaata acaataattc ccggacaatt
     2581 gtccccaatt cagcctctgt tctcgccaac gagtaaccat ataggcaagt aagtaaaaca
     2641 tgccaactgg catcgccaac ctggagaaga aaatataaat atggatcatt gtgctgccgc
     2701 tgtatcccga catcagcccg gaaattattg cgattgctat cggttcaggt gctattggct
     2761 gcacgatcgt tacggactcg cttttctggc tggtgaagca atattgcggc gctacgctca
     2821 atgaaacatt taaatactat acgacagcga catttatcgc ttcagtcgtc gctctggcgg
     2881 gcacattcct gctgtcattt atcatctaag cgcaaagaga cgtactatgg aaaacgctaa
     2941 aatgaactcg ctcatcgccc agtatccgtt ggtaaaggtt ctggttgctc ttaaagaaac
     3001 cacctggttt aatcctggca cgacctcatt ggctgaaggt ttaccttatg ttggcctgac
     3061 cgaacaggat gttcaggacg cccatgcgcg cttatcccgt tttgcaccct atctggcaaa
     3121 agcatttcct gaaactgctg ctactggggg gattattgaa tcagaactgg ttgccattcc
     3181 ggctatgcaa aaacggctgg aaaaggaata tcaacaaccg atcagcgggc aactgttact
     3241 gaaaaaagat agccatttgc ccatttccgg ctccataaaa gcacgcggcg ggatttatga
     3301 agtcctggca cacgcagaaa aactggctct ggaagcgggg ttgctgacgc ttgatgatga
     3361 ctacagcaaa ctgctttctc cggagtttaa acagttcttt agccaataca gcattgctgt
     3421 gggctcaacc ggaaatctgg ggttatcaat cggcattatg agcgcccgca ttggctttaa
     3481 ggtgacagtg catatgtctg ctgatgcccg agcatggaaa aaagcgaaac tgcgcagcca
     3541 tggcgttacg gtcgtggaat atgagcaaga ttatggtgtt gccgtcgagg aaggacgtaa
     3601 agcagcgcag tctgacccga actgtttctt tattgatgac gaaaattccc gcacgttgtt
     3661 ccttgggtat tccgtcgctg gccagcgtct taaagcgcaa tttgcccagc aaggccgtat
     3721 cgtcgatgct gataaccctc tgtttgtcta tctgccgtgt ggtgttggcg gtggtcctgg
     3781 tggcgtcgca ttcggtctta aactggcgtt tggcgatcat gttcactgct tttttgccga
     3841 accaacgcac tccccttgta tgctgttagg cgtccataca ggattacacg atcagatttc
     3901 tgttcaggat attggtatcg acaaccttac cgcagcggat ggccttgcag ttggtcgcgc
     3961 atcaggcttt gtcgggcggg caatggagcg tctgctggat ggcttctata cccttagcga
     4021 tcaaaccatg tatgacatgc ttggctggct ggcgcaggaa gaaggtattc gtcttgaacc
     4081 ttcggcactg gcgggtatgg ccggacctca gcgcgtgtgt gcatcagtaa gttaccaaca
     4141 gatgcacggt ttcagcgcag aacaactgcg taataccact catctggtgt gggcgacggg
     4201 aggtggaatg gtgccggaag aagagatgga gcaatatctg gcaaaaggcc gttaataaca
     4261 tttcaacgca gcatcgccat cctttccctg ggtgagcgat gctgccgatg gcgcagactt
     4321 aagatccccg gtcttacccg ctataacccc ctgcttttac catcacccaa cgcctttcgc
     4381 tgtaaacggc ggtttcgcaa accaaaccaa aaccgtaagt aagataaaag caatagccgc
     4441 catacggaaa atctcatttg cagaaataga aagtgactgc tgggttattt cattattaat
     4501 ttcattaaga actcctgaaa gcgaaccata gtatttatcc ataatttgtg acgatgaatt
     4561 aaacacaggg ttaaattgat cgatggttgc tgtcaactga ctatggtgta acgattcgcg
     4621 tcgcccccac agcgtcattg tcaacgacgt accaactgat cctgacaagg tacgaaaaaa
     4681 attactcata ctcgaggcat tggcaaattt attatctggc aaccctgaaa acgaaatcgt
     4741 tgttaagggt aaaaagaaac aggcaacggc gaacccctgg aaaaactgtg gcaaaatgat
     4801 gcctgtaaaa tcaatcgttg gcataaatgt cacagaacgc cagtaatagc aaaccgcata
     4861 catcaaaaaa ctaaatgtca ctaacaaccg catgtctatt ttgttgccat aacgtcctat
     4921 caaaggtgaa attaatagtg gcatgatgcc gatgggcgca taagcaagtc cggcccatat
     4981 cgcattatac cccatcgttt cctggagtaa ctgcggcata aggacgatcg ctccagagta
     5041 aaataaatac gcgcatgtga tactcactat accaatggtg aaattacggg acttaaacaa
     5101 actgagatca agaatcgggt tctctgaggt cgactcccaa atgactaaag agatcagaga
     5161 aataactgat actactgtta atattattat tgtactcgag ttgaaccaat ccagatcgcg
     5221 ccctttatca agcataattt gcaagccacc aacaccgagc actaacaggg tcagtcctgg
     5281 tagattcatt ttgaccggtg aagtctcagt ttctcttcct ttaagtaagg ttaagcataa
     5341 tgtcaggacg ataataccca tagggacatt gattaaaaat atccaacccc agctaaagtt
     5401 atcgcaaata tatccgccta atatcggccc acatatcgga gcgataatca cggtcattga
     5461 ccataatgcc agagcaaatg ttcttttttc tggtgggtaa ttccttaata ataaactctg
     5521 tgacagtgga attaacggcc ctgccattaa cccctgaacg actctaaaaa atatcaacac
     5581 atcaagattg gtcgataaac tacacattaa ggaagacagc gaaaaaaaag tgactgaaag
     5641 taaaaataac cttaattcgc ctattctttg tgccagcctg ccggtaacag ggatcgcaat
     5701 ggcatttgct acgccaaacg aggtgataac ccaggtgcct tcgtctgttg atgctcccag
     5761 aaagccagat attgtcggta ttgcgacgtt agaaatagtg gaatccaaca tttgcataaa
     5821 cgtcgctaat gacaatgcaa tagtgacgca ccataacgtc ccaccggtta atggtgccgg
     5881 agttgattta gtgattgcca ttaaagttgt ccattatgcg aaataatgtt gctaatttct
     5941 ttttctatcg gactggtatc gataactaaa gccttactgg tataagccgg catggacgtc
     6001 acggttgaag ccagctcagg catctcagcg atgtcttcgt tcttcgtatc aatagttgca
     6061 gtcatcgata aaccaatgcg caaggggtgt tccatgagtt cttttggatc aagagaaact
     6121 tcaaccggta cacgctgaac gattttgatc cagttccctg tcgcattttg tgcaggtaat
     6181 aaggagaacg cattgccggt tcccatattg atccctgtca cccggccatg aaacacaaca
     6241 ttctcaccat aaagatcgct gataatattg accgattgac caatccgtac atccgtgagt
     6301 tgtgtttctt taaagttagc attaacccac atttgacgtg ccggtacgac agccattaac
     6361 gattgtccgg gactcactgt ttcgccgacc tgaacacttc tctgggcaat atagccggta
     6421 accggactct taatatccgt acgtttaagc gccaaccagg cttctttggt tgcatccgca
     6481 gcttcaacga cttgtggctg acggtttaat ggtgtgttca ttactaaagc tttattcgct
     6541 ttataagcct ggatagcggc attcaatgcc gctttgctac ttattaacgt atctttggta
     6601 tgctccagcg tttcttttga aataaccccc tgcttcgcta aaggcacacg acggttataa
     6661 tcttctaaag attgttgata ctgaatacgt gctgaagcga cttcggcact gtattgttta
     6721 tcctgtaagt acagtttatt cgtttgccga acaatatttg ccagattatt tttagcttta
     6781 ttgagtgcga tagtggcatc agttttatcc agtgaaacta aaatgtcacc ctgtcgaacg
     6841 tagttcgtat ctttatgatt aacgacagtg acactacctg agacttgtgc agaaatggga
     6901 tctgcattcc ccgtgacata ggcgtcatct gtactaatca tgtcttctaa ttccattgac
     6961 caataggcat aagcacctga aaacgcaata aataaaacta tcgccaataa agaaaagtat
     7021 tttcttctgt tagaa
//
LOCUS       DAZUCE010000248         7031 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZUCE010000248 DAZUCE010000000
VERSION     DAZUCE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.248"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(28..2811)
                     /locus_tag="ACHY03_004400"
     CDS             complement(28..2811)
                     /locus_tag="ACHY03_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFS3907556.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATITPALLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVR
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPTAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPVQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISYLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2868..5240)
                     /locus_tag="ACHY03_004401"
     CDS             complement(2868..5240)
                     /locus_tag="ACHY03_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFS3907557.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5278..6963)
                     /locus_tag="ACHY03_004402"
     CDS             complement(5278..6963)
                     /locus_tag="ACHY03_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFS3907558.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLYQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1816 a         1612 c         1580 g         2023 t
ORIGIN      
        1 gccaacgatg tgttgacggg gctgttatta ttttggcaat aatactccag tataagtatt
       61 taccggatga gaaagatatt gtttaacggc agtgttaaca ttctctaccg tcatttgttt
      121 caacaattgc tcctgctcag tccatgctgc aggatcgtca tattgaataa gactatttac
      181 aatagtgttc gctaattgtt gaacgctacg ctgttggata tcgaggctgc gctgaacgtt
      241 ttgctggtat tcattcagtt cttgctcact gatcccttta gccagacgct taaccatcac
      301 ttcattcgct aacgttaaca gttcatcatg tcgttctggt tgacaagtaa aagccagcaa
      361 ataactgata tctttagcct gaggatcaac cgagaggcga gaagaaacgc tgtatgcccc
      421 agatgcctgt tcacgaatat taacgcgtag atcttttgcc agtgcgacgt taaaagcatc
      481 gagcgccata cgcgtcgcca gattaacagg tgtccgggaa tcataacgct tccactgtga
      541 aacctgtgcc acgggttcat tttgttcctt tacagtaacc gatgcgttgt ccgtcgcgcg
      601 agttaatggt ttacctgcgg ctaatggcga atcagagtgt ttgattgagc ctaagtaacg
      661 cgtaattaac gccacgagtt tgtcttctgc gacattaccg acaatgacaa acgtgatatc
      721 cgctggagat gaaaacaact ggcgatcggc agccagcgca tctgcggcag taaactgtgc
      781 aatctgattt tcttgcagta atttcgtgcg gtcatcagca tagcgcgtct catacatctg
      841 ctgggcgaat ttctccgctg gacgctggtc gagcgttttc aacgcctgaa tttgagcatt
      901 ttgtagcgat gcccaaatat tgtcgttaat cgtgctgtgg gtgattcgct ggttaatcaa
      961 ctgaaaacca ggttcagggt tattagtccg cgcgctaacg gagagcaacg tattcatgcc
     1021 actgacttta ctgctcatgg ttaccgaatt ttccgcactc cagcgtttca ggctggaaga
     1081 ggagagttcg ccaacgccgc ttccgctaac tgctttattt gccagcgcga taagtgattt
     1141 ttgctgcaca gggaaactta aatcgccttt attcgatacg gcaataattt gtagcttttg
     1201 ctcttcaccc gcggattttg ccagaataac ccttgcacca ttggaaagtg ttaatgatgt
     1261 cagattctca gccagcgttt ctttgctgct aatttccgcc tgtgggtcag cgtctactgt
     1321 taacgataaa tttctgcctg ggaagatgta agccgccagc tttttgttgg cgtactcttt
     1381 ttccagcgcc aggatagccg taggagacaa tgcttttttg gcggcaagct cattgtttac
     1441 catttgctcc caaaatgcgt cctggttctt tcttaactgc tgccattttt ccgccagact
     1501 ttgcacggta atttgctgcc acagacgttt cgaaagttga tatgtctctt ccggcgacaa
     1561 gaacggcgta ttatttaatg agcttgatgc caggcgactg gtcagcatac gtaaatcacg
     1621 ttcagcttgc tgatcaaccg catttttcag ccaggtgagg cgggtagatt tgacatcatc
     1681 gagttcttca gcagaaaaac catgctgatc aatggttgcc aactctgcca ttaatgcatt
     1741 cgcagcatcc tgcatattat cgtctcgtgc atttacacgg aaaaacagcg actgataatc
     1801 gggtgcaatt ctaacgctgc gcgcagtgcc gccagaaata gtcttcaact cgcccgactg
     1861 tatgcgttcc tgcagacgtt gattgaacag ctgaactaac atgctccatt cagcttgttc
     1921 gataaagctt tgctcatcgt tcacttgtac cattggcagg cgataataga gtgcgatgcc
     1981 gttcacccgg ttttctttat cattaatgat attaaagcgc aggtgatttt cggcttttgt
     2041 cggccagacg cgattttcag ctgctttgtt agccggaagc ttacttaaat tatcctttat
     2101 cagcgccagc gcttctttac tgtcgatatc gccgaccacg ataaaggtca tattatttgg
     2161 ttgataccag cgttgataaa attggcgcaa tagtgccggt gtgatcgtgg cgacagtatc
     2221 catcaggccg ataggttcac ggtctaaatt acgggtatta gccagcagga aagggcggcg
     2281 cgcctgagag gtgcgccatt tcgcatcctg atgggcacgc cattcctcag taattacgcc
     2341 acgttcagcg tctacttcga gtttttcaaa ggttgcggca ttactccatt cactgaagat
     2401 tgccatcact tgttgcagat tttgtttctg cgtagtcggc aaactcacct gatacaccgt
     2461 ttcgtcatag ctggtatagg cattaacatc gcgaccaaaa cgcaggccca ttgactcaaa
     2521 tgtttcgatg actttattac ccggccatgt ttttgtgccg ttaaacatca tatgttctac
     2581 aaaatgagcc acgccgcgct cattgtcttc ttcctgcaat gaaccggtat gaatttgcag
     2641 ccataaattt acctgatcct ttggttgagc atgcggataa atcatatatc gcaagccatt
     2701 gtccagttgt ccggtaatta acttttcatc ctgcggtaag gcggcggcaa tcagccgccc
     2761 ggggagcaac agagttgcca ctaacgtcag taagaaacag aggtttctca taattatctc
     2821 catgcgaaaa ccgggcgaat ttacccggtt aagtaaaatc cgaactatta aaatttcatg
     2881 ctgacatcaa gccagaaagt acggccactg gcgtaggtcg ccacaccggt attcgttgta
     2941 tcaaccgcgg ttttgctatc cagtacattg agaatatcgg cactgatcgt caggttttgt
     3001 tgtttcagaa attgtggcgt ccaggacaag cgggtgtccc aggtcaggct gctatccagt
     3061 ttttcgtcaa catactgctt gtaatctgaa tattcgctga tgtattgcgc attcgtctta
     3121 cccaggataa tgcgagcttt acgcgcttct tgccaggcca gcgtattcgc ccacaccaac
     3181 ccgctcggtt gatgcgtgaa atccatgttt aaggagatct ttaatgggtt attaaaatcc
     3241 gccactggaa cgctatcgta agaaaccaga ttgccgttat aaaccacctg gttatcaccg
     3301 gtattgctct cctcataacc attgtttaac gacaagttac ccttgctctt gatatagcta
     3361 aagacaattt gcgggttaat atctacctgg cggatatgca ggggttcggc cagttcaaaa
     3421 ctgaggttga acgaatgcgt tttggttttg ccgtcgttgt tatattcagt aatggtggtt
     3481 ttagtcgcgc tgtcggtacg actgcttttg ctgatttgat catgcgcttc acggtaaaca
     3541 tagtttgcgc gcgcaataac gttcttaccg attttttgct gcaatcccat tgccagttca
     3601 tcgttataag gcgttttcaa atcctgataa cgcgtcaggg ttttattacc tgataccgat
     3661 tccgtccagc tattgcggat atcacgtaat cccatatcaa gaatattccc gccatagtaa
     3721 cggttataac ctgctgtaat cattgaggtt tgatcagcaa aaatatccca ttccgtcata
     3781 aagcgcgggg agatattgtg gtttgacaga tagttgtcat agtcataccg cacgccgggc
     3841 attaatgaca cattacgcca gctaatgcga tccgccatat acagtgtgta gttgtcaatt
     3901 cccaggctgc ctttaccttt atggtaaatg gtatggttag ttttctttcc ggcagcatta
     3961 atcacatagg attcagactg gttatggcgt tcagtccacg catcggaata gatgtattcc
     4021 gcgccgaagt agggttgatg cgaaacatta cccacggcga atttttgcca gtccaggcgt
     4081 gttttgaagg tgtaattatc tacagcctgg gaaatgtgtc ctaatccgcc acgggtgcaa
     4141 cgccctgtaa tatcaccata tgtacatgaa agttcggtgt accagatgtc atgatcgtga
     4201 cgggtataat cactaatatg atcccaacca acggtggtac gtagtttggc ccatgcgagc
     4261 tgcgtatcca tatcccatgc cagaccatag gatttattac ccatttcgcg atcagactgc
     4321 gggaaggtgc tggtattata atcacggctg gagccggtat attttaaggt taaatcgtgg
     4381 gtaaagcggt cgctggcaaa ccaggtaaat ttgctcaatg cagtatcgat aacgttttta
     4441 tactgtgccc gaccggcgac aatgccgtca ttcgaaacat aatccgcgcg ggtgatatct
     4501 gactggcggc gcgataaacc ggcggtaacg ccaaagttat cagcgagttc ctgattaaac
     4561 gacaaggtat aaaagttctt tttaaaatct ggggagtaat aagtacttcc tgaagagcct
     4621 tgattaaatg cgctcttgtt attctcatcg atatgcgatg ttaaccagtc cgaacgcgta
     4681 gtgcgataac ccagtttcac gctgctatca tcagcgttga agcgtttgat ctttgcatca
     4741 attaccccgc cattgaagcg accgaattca accggcacaa aactgtcata aagcgtcaca
     4801 ttgtccagta agctgacatc aagataatac ccctgtgaca tcccgctaat attggttgca
     4861 ctactggcat cggactcatt cgctgggttc aggttattag tggcactaat accgtcaatc
     4921 aaataggcat tctgataggg cgacgcaccg tgaatagaga ttttctccgg gcgaatatct
     4981 ccctggttca acgaggtact ttgcgttgaa tccatgcgta ccgcagggtt ggttctcagc
     5041 agatcgctga tattgccatt cccggtcggt aaacgctcga tgctttcact ggtgtaatgc
     5101 gtgttgccgt ttacgggtac agggacagga gcatagaccg tcatctcttc aaaaacatgc
     5161 atgtacatgt ttttgtcatc gacggcctgc gccacatcag cgccacataa aatgacgcca
     5221 ggaataagaa ctcgcttcat ttatttatat ctccgtacag cagggctttt attgttttta
     5281 taaaaccgcg ctaatgtcac aaatatcatc tgccaggttc cagacaccgg gttgatgtgt
     5341 aaccataatg acgccgcttg tgggtagttt ttcacgcact aaacgcagta agcggatagc
     5401 ctcttgttcc tcaagatgag aggtagtttc gtcaagaaat atccatttcg gacgtcgtaa
     5461 aattaatcgt gccagggcga tacgttgttt ttcgccgctg gaaagaatat ctccccagcg
     5521 atcgtggtca tgaatacgcg cagccaattt ccccagacca acctgataca gtacttcgct
     5581 caacgattta tcgtctacgg gcaggggaag tgctttacaa ataatctctt tcagtaagcc
     5641 gcttttgatt aacggtgttt gtgacacata ccaactgtca gcaggagaag aaatatcacc
     5701 tttaaaccac ggccagcagt gggataatgt tttaagcagt gtggtttttc ccgcaccaga
     5761 gtagcctttc agtaatagcc atttgcctgg cgaaacatga aagttcaagt tctctaatat
     5821 gatcttatta tcaggcgtac gaatactcgc atcagccact tgtaccgcat gttggcaatt
     5881 tttaggctta ttcgtagggc gctgttcagt gagttgatgg aactcataca agcgatcgat
     5941 aaccgcagcc agttcagcaa gttcgtcata tttataaata aaccagctta aattattcga
     6001 taccagcata aaggcctggc gcgatttcat cagtccgccc agattgatct gtccgctaat
     6061 aaactgtggc aataacagaa agtagggaag aacgctaagc gagcgcgaat aaatattctg
     6121 ccagtaatca agccaccgct gacgattcat taaacgatgc cagttctctt taatggtatg
     6181 aaaattgtcg ctcaactcct ggcgttgtag agactcagca ttgcttagcg caatcagttc
     6241 tgcctgttta ttatgctgca caagattagt tcgaaatgtc gcttcgctac gttgtttttc
     6301 cacattaagc ggacgaatac gtttacctac cttatgggta aataaagttc caccgatcac
     6361 aatgagcacg acggtataga ccatataccc ctggatattc cattctgttc caccaacagt
     6421 gaatgagagc gtacccgcgc tttgccaaag aataacggta aaggtgatca gcatgctaag
     6481 tgactggatg aagccaaatg aaagactgag cgttttgctg atcagtaaga gaatgtcttc
     6541 agcgatacgt tggtcagggt tatctgtatt tttatgctca ccgtagatct gcgtgaagta
     6601 gtagttttta tctgcaaacc agcgattaag gtagtaatct gttaaccatt cgcgccagcg
     6661 aatagtcagc agtttaatta accatgtttt gtttacggat attaatacaa agatccccag
     6721 cagggcaggg aaccaaagga caagctgcca aagcttatcg gtttctttct ggcttagcgc
     6781 attgaaaaaa tcattattcc agtcgtttaa ccagacctga attttaacca cgccaaggat
     6841 catggcgagg ataataatga ttaacaacac tgaagtttta ttattcttac gcagccaaaa
     6901 tggcttcagc agacagagat actttgctat caacatacaa aacgtaatgg gaatggttat
     6961 cattagcgaa aattgattgt gccatccaat gattatctat ggcaatgatt tactgatgaa
     7021 atttatttaa g
//
LOCUS       DAZUCE010000249         6950 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZUCE010000249 DAZUCE010000000
VERSION     DAZUCE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.249"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(51..443)
                     /locus_tag="ACHY03_004403"
     CDS             complement(51..443)
                     /locus_tag="ACHY03_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3907559.1"
                     /translation="MAFASRLQRILKDLGWSQSELARHIGVTAQSVQAWCNGVTPRKD
                     KLDKLAKVTGYPVHFFFMNEGEYLDESTLHPGNYNQELTPQEQALLQLFRGLPESEKN
                     KLINELKEKREHFDLLLKELLEAKNQTK"
     gene            563..793
                     /locus_tag="ACHY03_004404"
     CDS             563..793
                     /locus_tag="ACHY03_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3907560.1"
                     /translation="MDKQLQDKILSVMSQSELGRRLGKKPQTVSLWFKGRVPGEEVLR
                     TSEALEWRVTPHDLRPDLYPNPDDSLPKTKAA"
     gene            852..1328
                     /locus_tag="ACHY03_004405"
     CDS             852..1328
                     /locus_tag="ACHY03_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS3907561.1"
                     /translation="MENQEELQKEILTWAARAGQELVTIEICRAWFSQGRNDELRLHE
                     FEDADGNVDWRAINNNRQKIFRWLRGETTAARRKTQVLASVMKAVLPAERRARLESPG
                     DPVLLATLAAKEGVEAINAVHLHFAPEITIQEIDEAIAALVATRGAVISTAQGHHA"
     gene            1368..1592
                     /locus_tag="ACHY03_004406"
     CDS             1368..1592
                     /locus_tag="ACHY03_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907562.1"
                     /translation="MLRHIDRITWRNGWHLNGRPAHVAEIRPIFDGRVAAARSVWEKY
                     EEEKAKLREQNLSGAAYEAGCRVLSEALGI"
     gene            1589..2368
                     /locus_tag="ACHY03_004407"
     CDS             1589..2368
                     /locus_tag="ACHY03_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS3907563.1"
                     /translation="MNILPLLDRPIAFQRSFIRLEMGVTAALFLSQLTYWTNRTTDDG
                     WVYKTQDEWEEETGLSRYEQEGARKKLRGLGILLERKQGLPARLYYKIDNDVLCQLLT
                     SAYKDAEKPHTGEGKTTRPVRGKPTNILTENTTEITTEITTEIITDGESADADTQQPV
                     DQRINYQVILDTYHEILPEMPPVKILTDSRKKQLRSFWKKFNFNESRWRAYLEFIGGN
                     CRWMLEDRPNGKGGFWRRKNLDYLITERCYVSVKEERANDK"
     gene            2358..3773
                     /locus_tag="ACHY03_004408"
     CDS             2358..3773
                     /locus_tag="ACHY03_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFS3907564.1"
                     /translation="MINNAIALYSVDAEQAVLGCLMLNTDHDRTNAVFALLKPETFYI
                     NAHQVIYREIRGLFQADKPTDLITLTEQMESKGLYERVGGFAYLAEMSKAAIPASMVN
                     YAKIVREKAILRYAVEKLNSCLEIMTQPADMTATDRITAVQQVIGQVAEHARTGHKGG
                     LRPASEVVDDWINDLDRRFNDPAHAAGLTLGIESLDRLMAPKQALRGSLVVIGARPKM
                     GKTAAFNKIAVHFALNHRLPTLVFSLEMTDRSLIERMVAQEAKVNSEIFYLGPNDESE
                     LSLAIAKAGEIAESNMMIDSTAGVTLAHIVAECRKVKRMRGSVGLVAIDYLTLMKTES
                     AERRDIAYGDITTGLKNLAKELDCVVVLLTQLNRKLEERADKRPNPSDSKDTGQIEQD
                     CDVWIGLYRDAVYNENADPHLMEMLLRLNREGPSGTAYALMKNGSVIDISDEEVYRRT
                     SDRSEKNKRYSRSDNSRTNEF"
     gene            3812..4222
                     /locus_tag="ACHY03_004409"
     CDS             3812..4222
                     /locus_tag="ACHY03_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907565.1"
                     /translation="MTTTPGKLEYPSDKGHIDDGKNYLDVILWNMNVGPRARTRAVFV
                     PRPKAGNFSTPAQTARQASVAAAPAVKRKGKTHTGIAIRRNGERQVKLHETATTWCAS
                     PHETYDKITGQRIGAPGRCRLLLSSITPIAKKGA"
     gene            4224..4460
                     /locus_tag="ACHY03_004410"
     CDS             4224..4460
                     /locus_tag="ACHY03_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907566.1"
                     /translation="MAGQSDYLPPGLPLNRAKWPQEYQLKEHYDMRAAALIRQLFEKR
                     IPRGSVIEQIGMTPDTYQEFFRERLNYWRGVMEQ"
     gene            4457..4768
                     /locus_tag="ACHY03_004411"
     CDS             4457..4768
                     /locus_tag="ACHY03_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3907567.1"
                     /translation="MKYKRWVRAEVVIIKQCAGSMTVERIGQLIGRTGAAVRTKAREL
                     KICMYLRGNYHQSAKYCQEDIELARELHQSGINRQDIAEKLEMPIGAVNQFVYFERRI
                     S"
     gene            4765..4989
                     /locus_tag="ACHY03_004412"
     CDS             4765..4989
                     /locus_tag="ACHY03_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907568.1"
                     /translation="MKELFLAFVPRFINDQIALTDNGEQYEIACSMVDVNPGERYDAM
                     CDLKIFTWLGWAIPCGEPTNIRPFESREAV"
     gene            5153..5410
                     /locus_tag="ACHY03_004413"
     CDS             5153..5410
                     /locus_tag="ACHY03_004413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907569.1"
                     /translation="MQTQLKVKGGYSHFFFANIEVSQRESNGGLVVVRDVNIPITTTT
                     LSLLENYAVGQVLTLEQKNGDFFDVYYVGTDGNNFQFSTHC"
     gene            complement(5623..6546)
                     /locus_tag="ACHY03_004414"
     CDS             complement(5623..6546)
                     /locus_tag="ACHY03_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFS3907570.1"
                     /translation="MRLKYSVLLLAIALTGCDNKKDVIGCSSEITQSALMDLLKKSAY
                     EGLSEQVDKYPDVTNQTKRSTLDKIKLAISEISTTSSDTGSTMKTCEGTVTMTLPANE
                     YIQLSDAYRKNFNRNLDKQMENLFLENNANAFSKRITYTVQATDDQKNVFVKTSSDNP
                     ISVGAALLTSLSIINPIVEQQKIQQAKDAQQSQIEAEQQVQLRAQEQARYEAEKQKER
                     QAQLQPQKSESLDQSRMAFANADSDLNTVWSKLTPAKKKELLPSQRQWIKTKDAMCGK
                     VSMQGTDAEVKKMVDCQTQMTLSRIAFLRNQ"
BASE COUNT         1924 a         1507 c         1783 g         1736 t
ORIGIN      
        1 agggggaaag ttgcatgcca gcagcggctg gcctggaaat ttgaactgaa ctattttgtc
       61 tggtttttgg cttcaagtag ttcttttagc agtagatcga agtgctctct tttttctttt
      121 agctcattga ttaatttatt tttttcgctc tcaggcagtc ctctgaatag ttgcagtaga
      181 gcctgttctt gcggggttag ttcttgattg taattacccg ggtgtagcgt ggattcatcc
      241 aaatattccc cttcattcat gaagaaaaaa tgaacagggt atccggtcac ttttgctagc
      301 ttgtctaatt tatcttttct tggagttacc ccattacacc atgcctgaac tgattgagcc
      361 gtaaccccaa tatggcgagc taactcagat tgagaccagc ctaagtcttt tagaatccgt
      421 tgtagtcggc tagcaaaagc catgtttttc gtttttgtgt tcataccgta aggatacaag
      481 gtttaattgt agggagcatt gcaaatataa tttgtaatta cagttatgat ttgtaatgat
      541 ggcgcggaca caaggagtcc atatggataa acaactgcaa gacaaaattc tttccgtaat
      601 gagtcaatcc gagttagggc ggcgattagg gaaaaaacca caaacggtaa gcctctggtt
      661 caaaggaagg gttcccggtg aagaggtttt gcgcacatct gaagctctag aatggcgcgt
      721 gaccccccat gatcttagac cggatcttta cccaaaccca gatgacagct tgcctaaaac
      781 gaaagcagct taactgttgg tgagccccaa atctgattaa gcgtaatcaa tttttagcga
      841 caggagacgc tatggaaaac caagaggaat tacaaaaaga gattttgacc tgggcggcaa
      901 gggcagggca ggaactcgtc acgattgaaa tctgccgggc ctggttcagt cagggacgca
      961 atgatgagtt gagactacat gaatttgagg acgcggacgg caatgtggac tggagagcca
     1021 tcaacaacaa tcggcagaaa atctttcgct ggttacgtgg cgagacaacg gcggcgcgcc
     1081 gaaaaactca ggtgctggcc agtgtgatga aagccgtgct acccgcagaa cggcgggcgc
     1141 gtctggagtc gccgggcgat cccgttttgc tggcaacgct ggcggcaaaa gaaggggtgg
     1201 aagcgattaa cgctgtacat ctccacttcg cgccagagat aactattcag gaaattgacg
     1261 aagcaatagc ggcgctggta gcgacgcgag gggcagtgat aagcactgcg caaggccatc
     1321 atgcatgagc gcttgaccag cgtttaactt ataccgagga aagaccgatg ttaagacata
     1381 ttgaccgcat tacttggcgt aacggctggc acctgaatgg gcgcccggca catgttgcag
     1441 agatccgccc catattcgat ggccgcgtcg cagccgcacg ttctgtgtgg gaaaagtacg
     1501 aagaagaaaa agcgaaactg cgtgagcaaa atctctctgg tgctgcctat gaggctggct
     1561 gtcgcgttct ctcagaggct ttgggcatat gaacatcctc ccgttacttg atagacccat
     1621 agccttccag agaagtttta tccgtcttga aatgggcgtg actgccgcat tgtttctgtc
     1681 gcagttgacg tactggacaa acagaacaac tgacgacggc tgggtatata aaacccagga
     1741 tgagtgggaa gaggaaacag ggctttcacg atacgagcag gagggcgccc gcaaaaaact
     1801 acgtggtctc ggcatcttgc tggaacgtaa acagggcttg cctgccaggc tgtactacaa
     1861 aatcgataat gatgtgttgt gccagctact tacgtccgca tacaaggatg cggaaaaacc
     1921 acatacaggg gaggggaaaa ccaccaggcc tgtacgtgga aaaccaacaa acattcttac
     1981 agagaatact acagagatta ctacagagat tactacagag attattactg atggtgaatc
     2041 ggctgacgcc gacacccaac aacctgtcga ccagagaata aattatcagg taatactcga
     2101 cacctatcac gagattttgc cagaaatgcc accagtgaaa atcctcactg atagcagaaa
     2161 aaaacaactt cgctctttct ggaaaaaatt caatttcaat gagtcccgtt ggcgggccta
     2221 tctggaattt atcggtggta attgtcgctg gatgctggag gacaggccga atggcaaggg
     2281 agggttctgg cgacgcaaga atcttgatta cctgattacc gaacgctgct acgtttctgt
     2341 caaggaagaa cgcgcaaatg ataaataacg cgattgcact gtacagcgtt gacgcagaac
     2401 aggctgttct gggctgtctg atgctgaata ccgaccatga cagaaccaat gcggtatttg
     2461 cgttactgaa accggaaacg ttttatatca atgctcacca ggttatctac cgggagatta
     2521 gaggattatt ccaggccgat aaaccaactg acctgataac actgaccgaa cagatggaat
     2581 cgaaggggct gtatgagcgc gtggggggat tcgcatacct ggctgaaatg agtaaggcgg
     2641 cgataccggc ctcaatggtc aactacgcga aaattgtacg tgaaaaagcc attcttcgtt
     2701 atgccgtgga aaagttgaat tcttgtctgg agattatgac ccagcctgca gatatgactg
     2761 ctactgacag gatcaccgcc gttcagcaag tgattgggca ggtagctgaa catgcccgta
     2821 ccgggcataa gggcggtttg cgtccggcaa gtgaagtcgt cgatgactgg ataaatgacc
     2881 ttgatcgccg tttcaatgat ccagcacatg cagccggact aacgctcgga atcgaaagcc
     2941 ttgatcgctt aatggcacca aagcaggcgt taagaggttc attggtcgtt attggtgccc
     3001 ggccaaaaat gggcaagaca gccgcgttta ataaaattgc ggtgcatttt gctctgaatc
     3061 atcgtttgcc gacgctggta tttagcctgg aaatgactga cagaagtctc attgaacgca
     3121 tggttgcgca ggaggcaaag gtcaattcgg agatttttta tcttggtcct aacgatgaat
     3181 ctgaattgag tctcgcaata gcaaaggcgg gggagattgc cgaatccaac atgatgattg
     3241 acagcacggc aggtgtaacc cttgcgcata ttgtcgctga atgtcgaaaa gttaaacgga
     3301 tgcgtggctc ggttggcctg gtggcaattg actatttgac gctgatgaag accgaatcgg
     3361 cagagcgtag ggatattgcg tatggcgata tcacaaccgg gctgaagaac ctggcaaaag
     3421 agctggattg tgttgtggtc ctgctgacgc aacttaaccg aaaacttgaa gaacgagcag
     3481 ataagcggcc taacccgagc gacagtaaag ataccgggca gatcgaacaa gattgcgatg
     3541 tctggattgg cctctatcgc gatgctgttt ataacgaaaa tgccgaccca catctcatgg
     3601 aaatgttgct acgtctgaac cgcgaggggc catcgggaac agcctacgcc ctgatgaaaa
     3661 atggctcagt tattgatatc agcgatgagg aggtttatcg ccgaaccagc gaccgctcag
     3721 aaaaaaataa gcgttattcc cgtagcgata attctcgcac aaatgaattt taaccacgtc
     3781 tgaccagcgt gaaataaccg aggaaagacc aatgaccacc actccaggca aattagagta
     3841 cccgtcggac aaaggacaca tagacgacgg taaaaactat ctcgacgtca ttttgtggaa
     3901 tatgaacgtc gggccacgcg cgcgcactcg tgcggttttt gtgccaagac ccaaagcagg
     3961 caatttttca acaccagctc aaactgctcg tcaggcatcc gtagccgcag cgccagcagt
     4021 gaaacgtaag ggcaaaacgc acaccgggat cgcgattcgc agaaatggtg agcggcaagt
     4081 gaaactccac gaaacagcga caacctggtg tgcctcaccg catgaaactt acgacaagat
     4141 aaccggacag cgcattggtg cgcctggtcg ctgtcgcctg ctgctgagtt ccatcactcc
     4201 gatagcaaag aaaggggcgt gatatggccg ggcaatcaga ttacctgccg cccggcttac
     4261 cactcaaccg cgccaaatgg ccccaggaat accagcttaa agagcactac gacatgcgag
     4321 cggcggcgct gatccgccaa ctctttgaga aacgtattcc gaggggaagc gtgattgagc
     4381 aaatcggaat gacgccggat acgtaccagg aatttttcag agagcgtctg aactactgga
     4441 gaggggtgat ggagcaatga aatacaaacg gtgggtgcgt gcggaagtcg taatcatcaa
     4501 acaatgtgca ggcagcatga cagttgaacg tatagggcag cttattggca gaaccggtgc
     4561 agcggtacgc acaaaagcgc gcgagctgaa aatctgtatg tatctgcggg gtaattatca
     4621 ccagtcagca aaatactgcc aggaggatat cgagctggca agggaattac atcagtcggg
     4681 tattaatcgc caggatatcg cagaaaaact cgaaatgccg atcggagcgg tgaatcagtt
     4741 tgtttatttt gagcggagga tttcatgaaa gaactttttc tggcatttgt gccccggttt
     4801 attaatgacc agatcgcact gactgataat ggtgaacaat atgaaattgc ctgcagcatg
     4861 gtggatgtga atccgggcga acggtatgac gcgatgtgtg acctgaaaat attcacctgg
     4921 ctgggttggg ccattccgtg tggagaacca acaaatattc gcccgtttga gagcagggag
     4981 gctgtatgag tgaattgaaa atacgaatga acccaagtcg caaggcgatc aaatttgcgc
     5041 actgtgtctt gcagatggct gaaggtgaat gttatataaa agagtgtcat ggataacaag
     5101 gcactcacta tcaatgatta tctgttaaaa aagcaatcga caaggagttg aaatgcagac
     5161 tcaattaaag gttaaaggcg gttatagtca ttttttcttc gccaatattg aagtctcaca
     5221 aagagagtca aatggcggtt tggttgtcgt tagggatgtt aatataccta ttacaacaac
     5281 aactctctct ctgttagaga attacgctgt agggcaggtg ttgacgttag aacaaaagaa
     5341 tggcgatttt tttgatgtgt attacgttgg aactgatggg aataactttc agttttcaac
     5401 gcactgctaa tttatcaagg ccctgtgagc agggccttcg aaagcctact aaagtctgtt
     5461 tcatagagag agttatttgc gattaaaaag gtatttggtc acaaccagca cgatagagaa
     5521 tacatctttc aaagtaccgc ccatataaaa cagttacata tgcacttgat gactaagctg
     5581 gcaccaggtt aacaccaggc gccagcttga aggagacttc agttattggt ttctgaggaa
     5641 agcgattctt gaaagggtca tttgcgtctg gcagtcgacc attttcttga cttcagcatc
     5701 tgttccctgc attgaaactt taccgcacat agcatcctta gttttgatcc actggcgctg
     5761 agaaggcagc aactcttttt tcttcgccgg ggttaacttg ctccagacgg tattcaaatc
     5821 agagtcagca tttgcaaatg ccattcggga ctgatcaagg ctttcagatt tttgcggctg
     5881 aagttgtgcc tgcctctctt tttgcttctc cgcctcatac ctggcctgtt cctgtgccct
     5941 gagttgcacc tgttgttccg cttcaatttg actctgttgg gcatccttag cctgctggat
     6001 tttctgctgc tcaacgatcg ggttgataat ggaaagcgat gtcagtaaag cggcgcccac
     6061 tgatatcgga ttatcagaag aggtttttac aaaaacgttt ttctgatcgt cggtcgcctg
     6121 cacggtgtag gtgatacgtt ttgaaaaggc gtttgcgttg ttctccaaaa acagattttc
     6181 catttgctta tcgagattac gattaaaatt ctttctgtaa gcatcagaaa gctggatgta
     6241 ctcgttcgca ggtagagtca tcgtcacggt cccttcacaa gttttcattg tgctgcccgt
     6301 gtcacttgat gttgtggaga tttcagagat ggctagtttg atcttgtcca atgtgcttcg
     6361 tttggtctga ttagtgacat cagggtattt atcgacctgt tcagagagtc cttcataagc
     6421 agattttttt aacaaatcca taagtgctga ctgggttatt tccgaagaac agccgatcac
     6481 atcttttttg ttatcacagc ctgtaagggc aatggcgagt aagagtactg agtattttaa
     6541 tctcatgaat tccctttaga gtaaggattg gtttttaaga cgcggtctgt aatcagttcc
     6601 gagagctaca gtaaacaacg ctgagcactg aacgcatcac aggcaaggta aaaggtgata
     6661 tcggcaaagg ctgtgaaacc tttaattcaa cagatgggaa cggtatgcac aggatcctgt
     6721 aacgacgctc ccaaaaccgg aaacctgcag ggcatgtttt cgctgctgaa cttaatatac
     6781 gaacctgcac tatcatgaac atattttata tcgttatacc ccgagtgatt gtgcccggca
     6841 atggatagtg tcctggagga actatttcta ctgtaaaagg tagacattta atttcagtgt
     6901 ggaattattc cctgctgtta ataaccatgg ctgtatatgg caccgagtag
//
LOCUS       DAZUCE010000250         6896 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZUCE010000250 DAZUCE010000000
VERSION     DAZUCE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.250"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..4463
                     /locus_tag="ACHY03_004415"
     CDS             <1..4463
                     /locus_tag="ACHY03_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFS3907571.1"
                     /translation="VQDGSHGVVNVTDKGHWNFLGTGEAFRHIYIGDAGDGELNVSSE
                     GKVDSGIITAGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNQGLVVSNGGS
                     SLGYGETGVGKVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKA
                     SGIGTLNLMDATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASGKGIVNI
                     STDSLWNLKTSSTNAQLLQVGVLGTGELNITTGGIVKARDTQIALNDKSKGDVRVDGQ
                     NSLLETFNMYVGTSGTGTLTLTNSGTLNVEGGEVYLGVFEPAVGTLNIGAAHGEAAAD
                     AGFITNATKVEFGSGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVIHDAGHTVFNAG
                     NTYSGKTLVNDGLLTIASHTADGVTGMGSSEVTIANPGTLDILASTNSAGDYTLTNAL
                     KGDGLMRVQLSSSDKMFGFTHATGTEFAGVAQLKNSTFTLERDNTAALTHAMLQSDIE
                     NTTSVKVGEQSIGGLAMNGGTLIFDTDIPAATLAEGYISVDTLVVGAGDYTWKGRNYQ
                     VNGTGDVLIDVPKPWNDPMANNPLTTLNLLEHDDNHVGVQLVKAQTVIGSGGSLTLRD
                     LQGDEVEADKTLHIAQNGTVVAEGDYGFRLTTAPGDGLYVNYGLKALNIHGGQKLTLA
                     EHGGAYGATADMSAKIGGEGDLAINTVRQVSLSNGQNDYQGATYVQMGTLRTDADGAL
                     GNTRELNISNAAIVDLNGSTQTVETFTGQMGSTVLFKEGSLTVNKGGISQGELTGGGN
                     LNVTGGTLAIEGLNARYNALTSVSPNAEVSLDNTQGLGRGNIANDGLLTLKNVTGELR
                     NSISGKGIVSATARTDVELDGDNSRFVGQFNIDTGSALRVNEQKNLGDASVINNGLLT
                     ISTERSWAMTHSISGSGDVTKLGTGILTLNNDSAAYQGTTDIVGGEIAFGSDSALNMA
                     SQHINIHNSGVMSGNVTTAGDVNVMPGGTLRVAKTTIGGNLENGGTVQMNTEGGKPGN
                     VLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTRVRVDNIGGVGAQTVNG
                     IELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWYLTSKWDGVTPADTPDP
                     INNPPVVDPEGPSVYRPEAGSYISNIAAANSLFSHRLHDRLGEPQYTDSLHSQDSASS
                     MWMRHVGGHERSSAGDGQLNTQANRYVLQLGGDLAQWSSNAQDRWHLGVMAGYANQHS
                     NTQSNRVGYKSDGRISGYSAGLYATWYQNDANKTGAYVDSWALYNWFDNSVSSDNRSA
                     DDYDSRGVTASVEGGYTFEAGTCSGSEGTLNTWYVQPQAQITWMGVKDSDHARKDGTR
                     IETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINNSKVYAVKMNGQTVS
                     RDGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQGMLGVKYSW"
     gene            4674..4940
                     /gene="hipB"
                     /locus_tag="ACHY03_004416"
     CDS             4674..4940
                     /gene="hipB"
                     /locus_tag="ACHY03_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFS3907572.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            4940..6262
                     /gene="hipA"
                     /locus_tag="ACHY03_004417"
     CDS             4940..6262
                     /gene="hipA"
                     /locus_tag="ACHY03_004417"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFS3907573.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LIPEDETVTRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            6533..6802
                     /locus_tag="ACHY03_004418"
                     /pseudo
     CDS             6533..6802
                     /locus_tag="ACHY03_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1878 a         1519 c         1904 g         1595 t
ORIGIN      
        1 gcgttcagga cggttcccac ggtgtcgtca acgtgaccga taaagggcat tggaatttcc
       61 tcggaacggg cgaagctttc cgccatatct atatcggtga tgctggcgac ggtgaactta
      121 atgtctcgag tgaaggcaaa gtagattcgg gaattatcac tgcggggatg aaagaaacag
      181 gcacaggcaa cattactgtt aaggataaga actccgttat cactaatctc ggaactaatc
      241 ttggttatga cggccatggc gaaatgaata tcagtaatca ggggcttgtt gtcagcaacg
      301 gaggaagttc actcggttat ggagaaaccg gcgtcgggaa ggttagcatc accacggggg
      361 ggatgtggga ggtcaataag aatgtctata ccaccattgg tgttgcgggc gtcggaaacc
      421 tcaatattag cgatggcggt aagtttgtat cgcaaaatat tacttttttg ggcgataaag
      481 caagcggtat cggcacactg aacctgatgg atgcgacatc atcgttcgat actgtgggta
      541 tcaatgtcgg taattttggt agcggtatcg taaatgtcag taatggtgcc acccttaatt
      601 caacgggcta tggatttatc ggaggaaatg cctccggtaa ggggatagtt aatatttcaa
      661 cggatagtct ctggaattta aagacatcat ctactaacgc ccaattgcta caggtcggtg
      721 tattaggcac gggtgaactg aatattacca ccggaggtat agttaaagcg cgtgatacac
      781 agatagctct caatgacaaa agcaagggcg acgtgagggt ggatgggcag aactctcttc
      841 ttgaaacatt caatatgtac gtagggacat ctggtacggg tacgttaacc ctgacgaata
      901 gcggcacgct gaatgtcgaa ggtggagaag tttacttagg tgtttttgaa cctgctgtag
      961 gaacgctaaa cattggtgca gctcacggtg aggcggcggc agatgccgga tttatcacca
     1021 acgcgacgaa agtagagttt ggctctggcg aaggtgtttt tgtctttaat catactaata
     1081 acagtgatgc cggctaccag gtcgatatgc tgattacagg tgacgataaa gacggaaaag
     1141 tgatccatga tgcaggccat acggtgttca atgcagggaa tacttatagc ggtaaaacgc
     1201 tggtcaatga cggcctcctg accattgcgt ctcatacggc agatggggta acgggcatgg
     1261 ggtcgagtga agtaaccatt gcaaaccccg gtacgctcga cattctcgca tcaacgaaca
     1321 gtgcaggaga ttacacgctg accaatgcgc tcaaaggcga tggcttgatg cgagtgcagc
     1381 tgtcatcctc cgacaagatg tttggcttta cccatgcaac agggactgaa ttcgccggtg
     1441 ttgcccaact gaaaaacagt accttcactt tggaacgcga caacaccgca gcgcttactc
     1501 acgcgatgtt gcagtctgac attgaaaata ccacatcggt aaaagttgga gagcaatcca
     1561 ttggtggact ggccatgaat ggaggtaccc tcattttcga tacggatatt cctgctgcga
     1621 cgctcgcgga gggatatatc agcgtcgata cgctggttgt cggcgcgggt gactacacct
     1681 ggaaaggccg taactatcag gtaaacggga cgggcgacgt gcttatcgac gtgcctaaac
     1741 cgtggaatga tcctatggcg aataaccctc tgacgacgct caatttgctg gaacacgatg
     1801 ataaccatgt cggcgttcaa ctggtgaagg cgcaaacggt tattgggtcg ggtggctcat
     1861 taacgttacg tgatttacag ggcgacgagg tggaagcgga caaaacgtta cacattgcgc
     1921 aaaacggaac ggtggtcgcc gagggtgatt atggattccg cctcacgacc gcaccaggtg
     1981 atggtttgta cgttaactat gggctgaaag cgctgaacat ccatggaggg caaaagctga
     2041 cgttagccga acatggcgga gcctatggcg caacggccga tatgtcggca aaaatcggtg
     2101 gtgaagggga tctggcaatc aatacggtgc gacaggtttc gctttccaac ggtcagaacg
     2161 actatcaggg ggcaacctac gttcagatgg ggacattacg taccgatgcg gatggcgcgc
     2221 tgggcaacac ccgggaactg aacatcagca acgcagccat cgtcgatctt aatggatcga
     2281 cgcagacggt agagacattc accgggcaga tgggttcgac tgttttgttc aaagaagggt
     2341 cgctgacggt aaataaaggt gggatcagtc agggtgaact gacaggtggc ggaaacctga
     2401 atgttacagg gggaacgctg gctatcgagg ggcttaatgc acgctacaat gcgttaacca
     2461 gcgttagccc aaatgcggaa gtcagcctcg ataatacgca ggggttaggc agaggaaata
     2521 ttgccaatga cggtctgtta acgctaaaaa acgtgactgg cgaactgcgt aatagcataa
     2581 gcgggaaggg tatcgtgagc gcaaccgcca ggacagatgt agagctggat ggcgataata
     2641 gccgctttgt ggggcaattc aacattgata caggcagcgc gctcagagtc aacgagcaga
     2701 aaaacctggg tgatgcttcc gttatcaata atggcctgct caccatctcc actgagcgta
     2761 gctgggcgat gacgcacagt atcagcggta gcggtgatgt gacaaaactg ggtaccggga
     2821 tcctgactct taacaacgat tccgcggcgt atcagggtac gacggatatc gtgggtggtg
     2881 aaattgcttt cggttccgac tctgccctta atatggcaag tcagcacatt aatatccata
     2941 acagcggtgt gatgtcggga aatgtcacca ctgcaggtga tgtgaacgtt atgccagggg
     3001 ggacactgcg tgtcgctaaa accacaatcg gcggcaacct ggagaatggc ggcacggttc
     3061 aaatgaacac tgaaggagga aaaccgggga atgtactgac cgttaacggc aactataccg
     3121 gaaacaatgg cctgatgacg ttcaacgcga cgctgggcgg cgataattca cccaccgata
     3181 agatgaacgt gaaaggcgat acccaaggga acactcgcgt tcgggttgat aacattggcg
     3241 gcgtcggtgc gcaaacggtc aacggtattg aactcattga ggttggcggt aattctgcag
     3301 gtaacttcgc gctgaccacc ggaactgtcg aagctggggc ttacgtctac acgctggcta
     3361 aagggaaggg gaatgacgag aaaaactggt atctgaccag taaatgggac ggcgtaacgc
     3421 cagcggatac acccgatccc atcaataatc cccctgttgt ggatccggaa ggcccatcag
     3481 tttatcgtcc ggaggccgga agctatatca gcaacattgc cgcagccaac tcgctgttta
     3541 gccatcgctt acacgaccgt ctgggtgagc cgcaatatac agattcactg cattctcagg
     3601 attcagcaag cagtatgtgg atgcgtcatg tcggggggca cgaacgttcc agtgccggag
     3661 acggccagct aaatactcag gctaaccgct atgtattgca gctaggcggc gatttggcgc
     3721 agtggagtag caacgcgcag gatcgctggc atcttggcgt gatggcaggc tacgccaatc
     3781 agcacagtaa tactcagagt aatcgtgtgg gttataaatc ggatgggcgc atcagcggtt
     3841 acagcgctgg gctgtacgcg acctggtatc agaacgatgc gaataagacc ggcgcttatg
     3901 ttgacagctg ggcgctgtat aactggtttg ataacagcgt cagttccgat aaccgttctg
     3961 ctgacgacta tgattctcgc ggtgtgacgg cctctgttga gggtgggtat acctttgaag
     4021 cgggaacatg tagcggcagc gaagggacgc tgaatacctg gtacgtccag ccacaggcgc
     4081 aaatcacctg gatgggtgtg aaagattctg accatgcccg gaaagacgga acgcgcattg
     4141 aaacggaagg cgacggaaac gtgcaaacgc gacttggggt gaaaacctac ctgaatagcc
     4201 atcaccagcg tgacgatggt aaacagcgtg agttccagcc ttacattgaa gcgaactgga
     4261 tcaacaatag caaagtctac gccgtgaaga tgaatggtca aaccgtaagc cgtgatggtg
     4321 cgcgtaatct cggtgaagta cgtaccgggg ttgaggcgaa agtaaataac aaccttagcc
     4381 tgtgggggaa tgtcggtgtg caactaggtg ataaaggcta tagcgatact cagggcatgc
     4441 tgggagtgaa atatagctgg taaacagtat aagccgcatg tcgagatggc atgcggctta
     4501 atattgccaa cttcaaaagg cttttaaagc ctccctttta tccgcgatcg cggatatctc
     4561 agcgtttatc ccgtacagcg gataagatgt gtttccagat tgactttatc ctcactaaag
     4621 gataaaactg ataatatccc cttaagcgga taaatttgct gtggacgtat gacatgatga
     4681 gctttcagaa gatctatagc ccaacgcaat tagcgaatgc aatgaaactg gttcgccagc
     4741 aaaatggctg gacgcagagc gagctggcga aaaaaatcgg cattaagcag gcgacgattt
     4801 ccaatttcga aaacaaccct gacaatacct cgctcacgac attttttaag attttacagt
     4861 cgcttgaact ctcaatgacg ctatgcgaca cgaaaaatgc ctcgccagaa tcaacagaac
     4921 agcaagatct ggagtggtaa tgcccaaact agtcacttgg atgaacaacc agcgggtagg
     4981 cgagttaacg aagttagcca acggcgcgca cacctttaag tatgcaccgg agtggttagc
     5041 cagccgttat gccagaccgt tgtcactttc gctgccattg cagaggggga atatcacctc
     5101 tgatgccgta tttaacttct tcgataactt gttacccgat agcccgattg tacgtgaccg
     5161 gatcgttaaa cgttatcatg ccaaatccag acaaccgttt gatttattgt cagaaatagg
     5221 gcgagacagc gttggtgccg tgacgttaat acccgaagac gaaaccgtaa cgcgtccgat
     5281 aatggcatgg gaaaagctta ctgaagccag acttgaagaa gtattaacgg cttataaagc
     5341 agatatcccg ctaggcatga ttagagaaga aaatgacttt cgcatctcgg ttgctggcgc
     5401 gcaggagaag acagcgctgc tcagaatagg caatgactgg tgcattccga aaggaataac
     5461 gccgacgacg cacatcatta aattaccgat tggcgaaatc aggcagccca atgcgacgct
     5521 cgatctcagc caaagcgttg ataatgagta ttactgtctg ctgctggcga aagaacttgg
     5581 gttgaatgtt ccggacgcag aaatcattaa agcgggaaat gtgcgcgcgt tagcggtcga
     5641 acgttttgac aggcgttgga atgctgagcg aacggtttta cttcgcttgc cacaggagga
     5701 tatgtgtcag acattcggtt taccttcatc ggtgaaatat gaatcagatg gaggcccagg
     5761 catcgcgcgg atcatggctt ttttgatggg gtccagcgag gcgctgaaag atcgctatga
     5821 ttttatgaaa ttccaggtct ttcagtggtt gattggcgca acggatggcc atgcaaaaaa
     5881 cttctccgta tttattcagg ctggcggcag ttatcgactc acgccatttt atgacatcat
     5941 ttcagcattt ccggtccttg gcggtacggg gatacacatc agcgatctca aactggcaat
     6001 gggacttaac gcatccaaag gcaaaaaaac ggcaatcgat aaaatttatc cgcgacattt
     6061 tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag atgcatgaaa tcctgagtga
     6121 ctttgccaga atgattccgg cagcactgga taacgtgaag acttcattac cgacagattt
     6181 tccagagaac gtggtgacgg cagttgaaac caatgtgttg aggttgcacg gtcggttaag
     6241 ccgagaatac ggtattaagt aagagtaaga tatggggttt ggtcgttgtt aatgagcagg
     6301 acaatcatga ccgccaacga ctagattctc ccttggttta tgttagtgcg cataatgtat
     6361 attatgttaa atcgcatctg agtgatacga agtgattttc cggtagtcgt tattcgttca
     6421 gggttccaga acttaagcct ctcctctttc ataattggcc tgtgcatgcc ggatataggc
     6481 tggctggtat gcagatttgc cgtttgccgc atcttcccag cgttgaagac tcaagctggt
     6541 aaaccgcgtg caaatgagca cgccctcttt ccatcaacac ttcaggcagc ttgcgggaat
     6601 gagtccactc cggtatcaga aatggctacg tctcaatgaa gtcaggcgaa cgatgctgaa
     6661 tgaacattac gatgtcacca cagcagccta tgctgtcggt tacgaaagct tatcccattt
     6721 cagccgggaa tatttgcgga tgtttggaga gtcacctaag agagatatta ccaggttaag
     6781 aaagtctgtc ggtcagcttt aataaaccct gcacttatct ggttagagca actcggtgtt
     6841 agtttcatca ggcaaacatt aaccatatct aatgattttt agccatataa actatt
//
LOCUS       DAZUCE010000251         6895 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZUCE010000251 DAZUCE010000000
VERSION     DAZUCE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.251"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            28..588
                     /locus_tag="ACHY03_004419"
     CDS             28..588
                     /locus_tag="ACHY03_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3907574.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLLPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     NTGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            674..1375
                     /locus_tag="ACHY03_004420"
     CDS             674..1375
                     /locus_tag="ACHY03_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3907575.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVTIRAAELLNNGKKIPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            1428..3950
                     /locus_tag="ACHY03_004421"
     CDS             1428..3950
                     /locus_tag="ACHY03_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFS3907576.1"
                     /translation="MNKSVVSISAAMLVLLCQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVAATNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDFTWQHGLTNTLTFNSG
                     SRIADGYQALMLGGVYGSTLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERQAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGVTFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSEKQVAP
                     VAGAAVKVVFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            3966..5300
                     /locus_tag="ACHY03_004422"
     CDS             3966..5300
                     /locus_tag="ACHY03_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3907577.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWATCSNTRVGQTEDGRSALI
                     EFGKINLTDTYFAPVGSLLATTVVPSTNYTSGGASGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFHNAGGPDGLSDVYATWFAFVGLKQTMAGVTIGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTAGATSYCGNTNADGDGVGYAKPSGTPYNCTQPNAY
                     IQLSGTSGIPFAHDEVGEDSADHYDFWGGDNGFGYGMRSVNRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTANFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAQPDTEMTLVGQPGIAKLTPGGNTAGW
                     YPVFEGATLEGATHPGYSGYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5297..5986
                     /locus_tag="ACHY03_004423"
     CDS             5297..5986
                     /locus_tag="ACHY03_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3907578.1"
                     /translation="MKSVLLLLTVLFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLVNNGSSS
                     YIELNNPTPLNISFTSISIGATKVEQESDMMIKPFSQRRYWLASKNDSGIVTATYLDD
                     DGAQIKQQLAQ"
     gene            complement(6130..>6895)
                     /locus_tag="ACHY03_004424"
     CDS             complement(6130..>6895)
                     /locus_tag="ACHY03_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFS3907579.1"
                     /translation="DFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANLLLVDIK
                     EPDNKYLKHLTHEGVEVEFMTIDITKPDATSTIINRCLERFGQLDILVNNAGVCNINR
                     PIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKIINMCSVLSFHGGRWSPGYA
                     ATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKGRIPAQR
                     WGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1838 a         1722 c         1622 g         1713 t
ORIGIN      
        1 ttaactctaa atttcaagga tattaatatg ttttctactt tcaaaaaagc agctctgctg
       61 gcagctatcg cattaccttt ttctaccatg gctgcaccga cagtcacttt tcagggtgaa
      121 gtaaccgatc agacctgttc cgttaatatc aacggtcaaa ccaattcagt agtattgctg
      181 ccgaccgtag ccatggctga cttcggtgca actttagctg atggccagag cgcaggccag
      241 acgccgttta cggtttctgt gtctaactgc caggctccga ctggtgcaga tcaggcaatc
      301 aacaccactt tcctgggcta cgacgttgac gctaacacag gtgttatggg aaaccgtgat
      361 accagcagcg acgcggcgaa aggctttggc attcagttaa tggattccag cacttctggt
      421 aacccagtaa ctctggctgg cgcgactaac gtaccgggtt tgaccctgaa agttggcgat
      481 actgaagcca gctacgactt cggtgcgcgt tacttcgtta tcgatagcgc agctgccact
      541 gccggtaaaa ttaccgctgt cgcagaatac accctgagct acctctaatt aatctactgg
      601 ctggcagtaa atgccagcca gtatttcttc gcacaattaa tagtgatttt tcatggacat
      661 ggaaaagata agaatgaaca tttttgcata tttactggta cttgtatttt ccatgagcat
      721 gagcagcagc gcgtttgcca gcgtggtaat gaccggaacc cgtattattt tccctggtga
      781 cgcaaaggaa aaaaccatcc agttgcgaaa taccagcgat cagccctata tcattaatat
      841 ccatgttgag gatgaacgtg gttctgacaa gaatgtaccg tttatgccaa ccccgcagac
      901 atttcgcatg gaagctgccg caggtcaggc gttacgcctg ctctacactg gtaataattt
      961 accgcaggat cgcgagtctg ttttctggtt tagtttcagt caactacctt atctgaataa
     1021 gaatgataaa agtcagaacc agctcatcct ggccctgact aatcgagtca aaattttcta
     1081 tcgtcccagc tcgattgtcg gtaaatccag tgacgcaccc aaaaacctga cttaccaggt
     1141 aaaacagaac cgcattgaag tgacgaatcc cacgggctat tacgtcacaa ttcgcgccgc
     1201 tgaactgctt aataatggta aaaaaatacc cctcgcgaat tcggtaatga ttgctcctca
     1261 aagcacaact gaatggacac taccctctgg catcagtgtc gctcccggtg cgcagatcca
     1321 tttagtgacc gtcaacgact atggcgtaaa tgttacgtct gagcatgcct tataacctct
     1381 ttctcctttt ttgagcggta tcctttttca cgcacgagta cgtcgagatg aataaatccg
     1441 ttgtgtcaat ttctgcggca atgttggttt tactttgcca accggtcatg gggagcgaaa
     1501 tctcacccgc aacaccgtca gatgaagaca actacacctt tgacccgcaa ctcttccgcg
     1561 gcagcagatt tagtcagtcg tcattagcaa aactgacaac acgtgagtct gttgcaccgg
     1621 gcaattataa aatggatatc tacaccaaca ataagttgtc aggcagttgg aatgtcacgt
     1681 ttaaagaagc cgctgatggt cgcgttctgc cctgcctgac gcctgaagtc gcggacgcga
     1741 taggcctcaa aacaggggaa gataaggggg aaaaagatcc tgtctgtacg tttgctaagg
     1801 aactcgctcc cggcatcacc agccagacac agttgtcaca attgcgcctg gacttatcgg
     1861 tgccacagag tcaattgatt agtcgccctc gcggctatgt tccccccagc gagctggata
     1921 ccggagcatc gctggcgttc atgaattata ttgccaacta ttacaacgtt gcctattcag
     1981 ggcagaatgc tcatagccag cgttcgctat gggcatcatt taatggcggc atcaaccttg
     2041 gtgcctggca atatcgtcag ttatccaaca tgacctggga taacgacaaa gggaatcagt
     2101 ggaacaatat tcgtagctat ttgcaacgcc cgctgcccgc cataaatagt caattaatga
     2161 tggggcaact tatcaccagc ggaagatttt tctctggact cagttatcac ggcgttagtc
     2221 tcgcgaccga tgaacgtatg ctgccggact ccatgcgcgg ctatgcgccg actattcgcg
     2281 gggtggcagc aacaaacgcc agagtctcgg taatgcaaaa cggtcatgaa atttatcaga
     2341 ctaccgtggc tcctgggcct ttcgaaataa acgacctata ccccaccagc tacagcggcg
     2401 atctggatgt caccgttacg gaagctaatg gcgcagtcag tcgtttcagt gtcccctttt
     2461 cagccgtacc agaatcgatg cgtccaggaa cttcccgtta taacgttgaa gtaggtaaaa
     2521 cgcaggatag cggtgatgac tcgatgtttg gtgacttcac ctggcagcac gggctgacta
     2581 atacgctaac atttaacagt ggctcgcgta tcgctgatgg ctaccaggcg ctgatgcttg
     2641 gcggagtcta tggcagtacg ctgggggcat ttggggcaaa cctcacttgg tcccatgcgc
     2701 gtgttcccga aagcgaagcg cagagtggtt ggatgtcgca attaacctgg agtaaaactt
     2761 tccagcccac gtctaccacc gtctccctgg caggttatcg atactctacc agcggctatc
     2821 gtgatctggc tgatgtgttg ggagagcgtc aagctgccag taataaacag tcatgggact
     2881 ccagccagtg gcgtcaacag tcgcgcttcg accttacgtt aagtcagagc cttgcgaatt
     2941 acggcaacct gtttgtatca ggttcaacac agaactaccg tggcggcaag agccgtgata
     3001 cacagcttca gttaggttac agcaatagct ttagccacgg catcagtatg aacctttccg
     3061 tcggacgcca aagaatgggc ggctataaag acaattctga tgatatgcag acggtaacgt
     3121 ccctttcatt ctcattccca cttggcggca atggacctcg tgtaccaagt cttagcaaca
     3181 gctggaccca ttcaactgac ggtagctcgc aattacaaag ctcgctaacc ggaatgcttg
     3241 atgaagcaca gaccaccaac tacagcctga acgtcatgcg cgatcaacaa tataagcaga
     3301 cgacgcttag cggaaacatg caaaaacgtt tttcacaaac taccgtcgga ttgaacgcat
     3361 cgaagggcca ggattactgg caggcgtccg gaaacgtaca aggcgcgatg gccgtacatg
     3421 gcggcggcgt cactttcgga ccttatctgg gtgaaacgtt cgccctggtc gaagcaaaag
     3481 gcgcagaagg tgcaaaagtc tataactcca gtcagctgga aattaatgac agtggttatg
     3541 cgcttgttcc ggcagtaacg ccctatcgct acaaccgtat atctctcgat ccacaaggaa
     3601 tggatggcga tgccgagctg gtcgacagtg aaaaacaggt agcaccggtt gcgggtgcgg
     3661 cggtgaaagt tgtcttccgt acccgtcctg gtaaagcgct gctgattaaa tcccgcatgg
     3721 cagatggttc ggaactgcca atgggagccg atgtgctgga tgagaataat actgttgtcg
     3781 gtatagccgg tcaggggggg caaatttacc tccgcacaga acagacaaaa ggccatttgt
     3841 cagttcgctg gggtgaaggc gctaacgata gctgccagtt gccctttgat atcagcggga
     3901 aggacagcaa tagccctatc atccgcctga atgaaacctg tcagtcttga tacagagaaa
     3961 acccgatgtc taaatcgact tttttacaca ttctgattag ctccatcata ttggtggcac
     4021 taatacaatc atccgcctgg gccacttgtt ctaatacacg tgtcggacaa acggaggacg
     4081 gaagatcggc actcattgag ttcggaaaaa ttaacttgac cgacacctat tttgcgccag
     4141 taggctcact actcgcgaca acggtcgtac cttccactaa ctacacatca ggtggcgcgt
     4201 caggaagctc cgtactgtgg gaatgtgacg caacagattt gccaaacatc tattttctgg
     4261 tcgcaaccaa tggcgacgac cgcgtgggtg gctttcacaa tgcaggcggg cctgatggtc
     4321 tgagtgatgt ctatgccacc tggtttgctt ttgtcggtct caagcagact atggcgggcg
     4381 tgacgattgg tcgttactgg aagaaagtgc ccatcaccag ttatgccact cagggaacta
     4441 aaatccagat ccgcttacag gatatccctc ctcttcatgc tgagctttat cgtattagta
     4501 cgctgcctga taccgctggc gcaaccagtt actgcggtaa taccaatgct gatggcgacg
     4561 gtgtggggta tgcgaaaccc tccggcacac cttataactg tacccagcct aatgcctata
     4621 ttcagctttc cggtaccagt ggcattccat ttgctcatga tgaagtgggc gaggacagtg
     4681 cggatcacta tgatttttgg ggcggtgata atggttttgg ttacggaatg cgatcggtga
     4741 atcgacttta caacaatgcc acctgcgttg cccgcagcgc cacgccgtta gtattgctgc
     4801 cgacaattgc agaagcacaa ctgaatgcag gcatggaaag taccgctaat tttaatgtcc
     4861 gcgtcgagtg tagtaactca gttcaatcag ggattagtga tactcagaca gcattaggaa
     4921 tccaggtgtc tgaaggtgca tatacagcag cgcaaaaact ggggattatc aatagcaacg
     4981 gcggcgtcag cgccttagtc tcagataatt atgacgcggc agagatggca aaaggcgttg
     5041 ggatctatat ttctaacagt gctcagcccg atacggagat gacgctggtt ggtcaacctg
     5101 gcatcgcaaa gttaaccccc ggaggaaata ccgcgggatg gtatccagtc tttgaagggg
     5161 caacattaga aggtgcgacg caccccggat actccggcta tagttactct tttatcgccc
     5221 ggttgaagaa actgccaaat cagacagtca gtgcgggaaa agtgcgggca acggcttata
     5281 tattggtgaa aatgcaatga aatccgtttt gctcctctta accgtcttgt tcgtacagtc
     5341 agtggaagcc ggcatcatgc cggcttcttc acggattatt tatcgcgatg gcgatcggga
     5401 aaagacgctg atgctggtca ataccaatga ctatccggta ctggtgcaaa gttgggtaga
     5461 taatggtgaa ggttccccag aagctgcttc tccccccttt gtggtgctgc caccggtttt
     5521 ccgcctggct gcgagacgaa cccaggggtt gcggattatc tataatcatg acccacttcc
     5581 ccaggatcgc gaatctgcat tctggttaaa tctttatgaa atgccaccag aaactcccca
     5641 tcaagatccc acacatttaa cgctggcaat gaatacgcaa cttaaaatct tttacaggcc
     5701 aaaatctgta actatgacgc cccaagaagc tatgcgtaaa ataagttggc atctggttaa
     5761 caacggcagc agcagctata ttgagcttaa taacccaaca ccactgaata tctctttcac
     5821 ctccatcagc attggggcta cgaaggtaga gcaagaaagc gatatgatga taaaaccctt
     5881 cagtcaacga cggtactggt tagcaagtaa aaacgatagt gggatcgtta ctgcgacata
     5941 tctcgatgat gatggtgcac agataaagca acaattagcg caataatacc ccgcgtggat
     6001 atgtaaactc ttcgcgatag ttcaccacta acgaacaaca tctatacccc tgtccccccg
     6061 aagtggagaa atgacaaaat ctctccactc cattttccag ttaaaacacc tcaacgcagc
     6121 caaaaatcgt taacggattg aatatccgcc atcaatgact aatagttgtc cattgacata
     6181 atctgaagcg gcactggcga gaaacaccat tgcgcccata agatcctgtg cccttcccca
     6241 gcgttgagcc ggtatacgcc cttttattaa ctcttttatt ttagggttat tataaataat
     6301 cgctgtcatt tcactcacat aatatcctgg ggcgatacca ttgatctgaa tattatattc
     6361 cgcaaaatca tcggcatacg ctttggtcaa ccccgctaaa gcatgtttag tcgcagcata
     6421 ccctggagac caacgtcccc catgaaatga caatacggaa cacatattaa taattttccc
     6481 ttttctttgc gggacaaata tattcaatgc ggcctggctc atatcgaatg cagcattcag
     6541 gttgagatta atcatcggat cccagtcgtt tcgattgaaa tcgataggac gattgatgtt
     6601 acacacaccc gcgttattga ccagaatatc taactgaccg aaacgttcca gacacctatt
     6661 aataatggtg cttgttgcat caggttttgt tatgtctatt gtcataaact caacttcaac
     6721 gccttcatga gttaaatgct ttaaatattt attatcaggc tctttgatgt ctaccaacaa
     6781 cagattagct cctgctttgg ccagagcaga gcataagcct ctccccagtt cacccgcggc
     6841 cccagttacg atggccacct tgttatgtaa agaaaaaaaa tccaatgaaa aatct
//
LOCUS       DAZUCE010000252         6864 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZUCE010000252 DAZUCE010000000
VERSION     DAZUCE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.252"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            215..1198
                     /locus_tag="ACHY03_004425"
     CDS             215..1198
                     /locus_tag="ACHY03_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFS3907580.1"
                     /translation="MPEKYSPDDQSSTIDPYLITSVRQTLEEQGAALQNLSKQLDSGQ
                     YQCVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PCDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1270..2418
                     /locus_tag="ACHY03_004426"
     CDS             1270..2418
                     /locus_tag="ACHY03_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFS3907581.1"
                     /translation="MLIKVKSAVSWMRAHLSAISLSDIQKHLVKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2442..4118
                     /locus_tag="ACHY03_004427"
     CDS             2442..4118
                     /locus_tag="ACHY03_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFS3907582.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDISADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4128..4868
                     /gene="kdsB"
                     /locus_tag="ACHY03_004428"
     CDS             4128..4868
                     /gene="kdsB"
                     /locus_tag="ACHY03_004428"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFS3907583.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            4865..>6864
                     /locus_tag="ACHY03_004429"
     CDS             4865..>6864
                     /locus_tag="ACHY03_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFS3907584.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLSVNGAPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISKAREAMHTIVTGDLSKYNLAPAFVADESECSDIVLVVDQTFND
                     MSVTYGNAGQNEFSAMLEAAMAENPQATIWVKVHPDVLKGKKTGYFADLRATQRVRLI
                     TENISPQSLLRHISRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSVTLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLLRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLRTATNRLSYSRRCTAATACVVWGVKGEQQWQVEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPSLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKGKKVILVPGQVEDDSSIKTGTISIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAELIAELADYQALDADIIQCIQHADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTFDEHHCGRRERELTLADLLYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTSRKPMFITRKKAGWVIRYYRKL"
BASE COUNT         1742 a         1655 c         1803 g         1664 t
ORIGIN      
        1 tgataaaaat catgtgggac gggcatgtgc ccctcccttt cctttattat gtctgataat
       61 gaaacgctaa aattattttt gtttgtagac tgtgttatat gctttatgga aaagtagtat
      121 tttgatttat gtgaagatga gctgaactcg atttatcaga ggcaagttgt ctttatgtta
      181 taagtgtttt aaccttatcg gaagtataat ttatatgcca gaaaaatatt cacctgatga
      241 tcagagcagt actattgatc catatttaat tacttctgtt cgtcagacct tggaagaaca
      301 aggtgcagca ttacaaaact tgtctaaaca actggattcc gggcagtacc agtgtgtcct
      361 taatttgata atgaactgta aagggcacgt tattctttcg ggaatgggta aatcagggca
      421 tgtcggtcgt aaaatgtcag cgacgctggc ctctaccggt accccgagtt tctttattca
      481 tcctgcagaa gcgttccatg gcgatctggg tatgattacg ccctgcgatc ttttgatcct
      541 tatttctgcc agcggtgaaa cggatgaaat cctcaagcta gttccttcac tgaaaaattt
      601 cggcaaccga attatcgcca ttaccaataa tggaaattcc acgctggcga aaaatgctga
      661 tgccgtgctg gaactccata tggcgaatga aacctgcccg aataatcttg caccaacaac
      721 gtctaccacc ctgaccatgg cgatcggcga tgcgctggcg attgccatga tccaccaacg
      781 caaatttatg ccgaatgatt ttgcgcgcta tcacccgggc ggttcattag gtcgtcgcct
      841 gctgacccgc gttgctgatg tcatgcagca tgatgttcct gcggtacagc tggatgcgtc
      901 atttaaaacc gtgattcaac gtatcaccag cggatgccag ggaatggtga tggtagaaga
      961 cgcagaaggt gggctggcgg gcattataac cgacggtgac ctgcgtcgct ttatggaaaa
     1021 agaggattct ctgacatccg ctacggctgc gcagatgatg acacgtgaac cgctgacgct
     1081 accggaagac accatgatca ttgaagcgga agaaaaaatg caaaagcacc gcgtctcaac
     1141 gttattggtg accaacaagg caaataaagt cactggcctt gtgcgcattt tcgactaatt
     1201 aagcaacggg gtttgagagg ttagtctctc attgagtttg tgccccactg gaactttgat
     1261 aatcgttaca tgttgataaa agtgaagtct gccgtatcct ggatgcgtgc tcatctgtct
     1321 gccatctcac tgtcagatat ccaaaaacac ctggtgaaaa tcatcattct ggcaccgatg
     1381 gcggtgctgc tgatctatct ggctatcttc agccagcctc gctatatgag cgagtcgaaa
     1441 gtcgccatta aacgctcgga tgatttaaac agcggcagcc tgaattttgg tctgcttctg
     1501 ggtgcctcta accccagttc cgcagaagat gcgttgtatc tgaaagagta catcaactcg
     1561 ccggatatgc tggcggcgct ggataaacaa ctaaattttc gtgaagcgtt cagccacagc
     1621 gggctcgatt ttcttaatca tcttagcaag gatgaaaccg cagaaggctt cctgaagtac
     1681 tacaaggacc gtatcaacgt ctcgtatgac gataaaaccg gattactgaa tattcagacg
     1741 cagggcttta gcccggagtt tgcgcttaag tttaaccaga ccgtgctgaa agagtcagag
     1801 cgctttatca atgagatgtc acatcgcatc gcgcgtgacc agcttgcctt tgcagaaacg
     1861 gagatggaaa aggcacgcca gcgtctggac gccagcaaag cggaattgct ctcttatcag
     1921 gacaacaaca acgttctgga tccacaggca caggcacagg cggcgagcac gttagtcaat
     1981 acgctgatgg gccagaagat ccagatggaa gcggatctgc gtaacttgct gacgtatctg
     2041 cgtgaggacg ccccgcaagt ggtgagtgcg cgtaatgcga ttcagtcatt acaggcacaa
     2101 atcgatgaag aaaaaagcaa aatcactgca ccacagggtg acaagctaaa ccgtatggcg
     2161 gtggattttg aagaaatcaa atcaaaagtt gagttcaaca ccgagctcta caaactgacc
     2221 ctgacctcca ttgaaaagac ccgtgtagaa gcagctcgta agctcaaggt gctgtcagtg
     2281 atcagttcgc cacagttgcc gcaggaatcg tcttttccaa atatccctta tttgatcgcc
     2341 tgctggttac tggtgtgctg cctgctgttc ggcaccctga aactgttgct ggctgtcatt
     2401 gaagatcacc gagactaacg ctgtcgctga atgagtttgt gatgaaatta tttaaatcaa
     2461 ttttactgat tgccgcctgt cacgcggcgc aggccagcgc ggccattgat attagcgctg
     2521 acccaaacct gacaggagcc gcgccgctta ccggtattct gaacggacag aagtcggata
     2581 cgcaaaacat gagcggcttc gacaataccc caccgcccgc accgccggtg gtaatgagcc
     2641 gtatgtttgg tgctcaactt ttcaacggca ccagcgcgga tagcggtgcg acggtaggat
     2701 tcaaccctga ctatattctg aatccgggtg atagcattca ggttcgcttg tggggtgcgt
     2761 tcacctttga tggtgcgtta caggttgatc ccaaaggtaa tattttcctg ccgaacgttg
     2821 gtccggtgaa agttgctggc gtcagtaata gtcagctaaa tgccctggtc acatccaaag
     2881 tgaaggaagt ataccagtcc aacgtcaacg tctacgcctc cttattacag gcgcagccag
     2941 taaaagtgta cgtgaccgga tttgtgcgta atcctggtct gtatggcggt gtgacgtctg
     3001 attcgttact caattatctg atcaaggctg gcggcgttga tccagagcgc ggaagttacg
     3061 ttgatattgt ggtcaagcgc ggtaaccgcg tgcgctccaa cgtcaacctg tacgacttcc
     3121 tgctgaacgg caaactgggg ctttcgcagt tcgccgatgg tgacaccatc atcgtcgggc
     3181 cgcgtcagca tactttcagc gttcagggcg atgtctttaa cagctacgac tttgagttcc
     3241 gcgaaagcag cattcccgta acggaagctt taagctgggc gcgccctaag cctggcgcga
     3301 ctcacattac gattatgcgt aaacaggggc tgcaaaaacg cagcgaatac tatccgatca
     3361 gttctgcgcc aggtcgtatg ttgcaaaatg gcgatacctt aatcgtgagc actgaccgct
     3421 atgccggtac cattcaggtt cgggttgaag gcgcgcactc cggtgaacat gccatggtac
     3481 tgccttatgg ttccactatg cgtgcggttc tggaaaaagt ccgcccgaac agcatgtcgc
     3541 agatgaacgc ggttcagctt tatcgcccat cagtggctca gcgtcagaaa gagatgctga
     3601 atctctcgct gcaaaaactg gaggaagcat cactttctgc ccagtcctcc accaaagaag
     3661 aagccagcct gcgaatgcag gaagcacaac tgatcagccg ctttgtggcg aaagcacgca
     3721 ccgtggttcc gaaaggtgaa gtgatcctca acgaatccaa tattgattct gttctgcttg
     3781 aagatggcga cgtcatcaat attccggaga aaacatcgct ggttatggtt catggcgaag
     3841 tgctgttccc gaacgcggtg agctggcaga agggtatgac caccgaggat tacatcgaga
     3901 aatgtggtgg cctgacgcaa aaatcgggta acgccagaat tatcgtcatt cgtcagaacg
     3961 gtgctgccgt caacgcggaa gacgtggatt cactcaagcc gggcgatgag attatggttc
     4021 tgccgaaata tgaatcgaaa aatattgaag ttacccgtgg tatttccaca atcctctatc
     4081 agctggcggt gggtgcaaaa gtgattctgt ctttgtaagg agttgagatg agcaaagcag
     4141 ttattgtcat cccggctcgc tatggctcct cgcgcctgcc gggtaagcca ctgctcgata
     4201 ttgttggtaa accgatgatc cagcatgttt acgagcgtgc gttacaggtg gcgggcgtcg
     4261 cagaagtttg ggtggcaaca gacgatcagc gtgttgaaaa ggccgtgcag gcatttggcg
     4321 ggaaagccat catgacgcgc aacgatcatg aatccggcac cgatcggctg gtcgaggtga
     4381 tgcataaagt cgaggcagat atctacatta acctgcaggg cgacgaacca atgattcgcc
     4441 cgcgggatgt agaaacgctg ttacaaggaa tgcgtgatga ccccgcgttg ccagtggcaa
     4501 cgctatgcca cgcgatttct gccgaagaag cggctgagcc aagcacggta aaagtggtgg
     4561 taaataccca ccaggatgcg ctttatttca gccgttcgcc gattccgtat ccgcgtaatg
     4621 ctgaaaaagc gcgctacctg aaacacgttg gtatttacgc ttatcgtcgc gatgtgctgc
     4681 aaaactacag ccagttaccg gagtccatgc cagagcaggc agaatcactg gagcagctgc
     4741 ggttaatgag cgcggggatc aacatccgca catttgaggt tgccgcaacc ggtccgggcg
     4801 tcgacacccc agcatgcctg gaaaaagtgc gcgccctgat ggcacaggaa ctggctgaaa
     4861 acgcatgatt ggcattttct cgtccggtat ctggcgtatt ccgcatctgg agaaatttct
     4921 ggcgcaaccg tgccagaaac tttctctgct gcgccctgtt ccgcaagaag ttgatgctat
     4981 cgccgtgtgg ggacatcgtc ccagcgcggc aaaaccggtc gccatcgcca aagcagcggg
     5041 aaaacccgtc atccgtctgg aagatggatt tgtgcgttcg ctggatctta gcgtcaatgg
     5101 tgcgccgccg ctttctctcg tggtggatga ttgtggcatt tactacgatg ccagcaagcc
     5161 ttcagcactg gagaaactgg tacaggataa agccggaaat acagctctga taagtaaggc
     5221 cagagaggcg atgcacacca tcgtgaccgg ggatttgtcg aaatataacc tggcgcctgc
     5281 gtttgtggct gatgagtcag aatgttcaga catcgttctg gttgtcgatc agacatttaa
     5341 tgatatgtca gtgacgtatg gtaatgctgg tcagaatgag ttttctgcca tgctggaggc
     5401 cgcgatggcg gaaaatcctc aagcgacaat ttgggtgaag gtgcacccag atgtactgaa
     5461 aggaaagaaa acaggttatt tcgccgatct gcgcgccacg caacgagtac gtttgattac
     5521 ggagaatatc agtccgcagt cgctgttgcg acatatttcc agagtttacg tcgtgacctc
     5581 ccaatacggc tttgaggcct tactggcagg aaaaccagtg acatgcttcg gtcaaccctg
     5641 gtatgcaggc tggggcttaa cggacgatcg tcatccgcag tcggctttgt tatctgcccg
     5701 acgcggttct gtcacgctgg aagaactttt tgccgcagca tacctgcgtt actgtcgcta
     5761 tatcgaccca ctaacggggg cagcaagtga cctgtttacc gtactgcaat ggctgcaatt
     5821 actgcgcaac catcagcaac agcgtaatgg ctatttatgg gcgccaggct taacgctgtg
     5881 gaagtcagca atcctgaaac ctttcttgcg taccgcgaca aacaggctga gttattcacg
     5941 tcgctgtact gctgccaccg cctgcgtggt atggggtgta aaaggagaac agcaatggca
     6001 ggtcgaagcg cagcgaaaat cactgccatt atggcgaatg gaagatggtt ttctgcgttc
     6061 atccggactt ggctcggacc tgctgccatc gctatcgctg gtgctggata aacgcggaat
     6121 ttactatgac gccacgcgcc ccagcgacct ggaagtgctg cttaatcaca gccagttaac
     6181 gctggcgcag cagatgcgag ctgagaagtt gcggcaacga ctggttgaaa gcaaactgag
     6241 taagtacaac ctgggagccg atttctctct gcctgccgaa gccaaaggta aaaaagttat
     6301 cctggtgccg ggtcaggtag aggacgattc ctctattaaa acaggcacta tctcaattaa
     6361 gagcaacctt gagttattac gcacagtacg cgagcgtaat ccgcacgcct acattattta
     6421 taaaccgcac ccggatgtac tggtggggaa tcgcaagggc gatattccgg cagaactgat
     6481 tgctgaactt gctgattatc aggcactgga cgcagatatt attcaatgca ttcagcacgc
     6541 agatgaagtg cataccatga cgtcgctgtc ggggtttgaa gcgttattac atggtaagca
     6601 cgtacattgt tacggcctgc ccttctatgc tggttggggt ttaaccttcg atgaacatca
     6661 ctgcgggcgt cgtgagcgag aattaacgtt agcggatttg ctatatcagg cgctgattgt
     6721 ttatccaact tatatccacc caacacggct acaacctatt actgttgaag aggcagcgga
     6781 atatttgatc cagacgtcgc gcaagccgat gtttattacc cgaaaaaaag cgggatgggt
     6841 aatacgctat taccgcaaat taat
//
LOCUS       DAZUCE010000253         6808 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZUCE010000253 DAZUCE010000000
VERSION     DAZUCE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.253"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(238..723)
                     /gene="sixA"
                     /locus_tag="ACHY03_004430"
     CDS             complement(238..723)
                     /gene="sixA"
                     /locus_tag="ACHY03_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFS3907585.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGEFNWQMSPCNLKMAKA
                     I"
     gene            complement(926..3070)
                     /gene="fadJ"
                     /locus_tag="ACHY03_004431"
     CDS             complement(926..3070)
                     /gene="fadJ"
                     /locus_tag="ACHY03_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFS3907586.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLDGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3070..4380)
                     /gene="fadI"
                     /locus_tag="ACHY03_004432"
     CDS             complement(3070..4380)
                     /gene="fadI"
                     /locus_tag="ACHY03_004432"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFS3907587.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4560..4844)
                     /locus_tag="ACHY03_004433"
     CDS             complement(4560..4844)
                     /locus_tag="ACHY03_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFS3907588.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5216..6556
                     /gene="fadL"
                     /locus_tag="ACHY03_004434"
     CDS             5216..6556
                     /gene="fadL"
                     /locus_tag="ACHY03_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFS3907589.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTQQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1702 a         1832 c         1608 g         1666 t
ORIGIN      
        1 gacacaaaaa tatcatttct tttttactgt taattgttcc attagcttta taatttgtaa
       61 gaatatatta caacttagtc tcatttcatc agaaattaat agggaagcag aggaaagagt
      121 gcgatgaaat catcatcgca gcataccaac tccgtgaagg gtttttagtg aatttatact
      181 attgcaaaaa gacaaatgcc cgatgtacag cttacatcgg gcattttttc aaacgaatca
      241 gatagctttc gccatcttca aattacacgg gctcatctgc cagttgaact cgccttttcc
      301 gctttcatca agcgttacgc tggcaatggc cgaggtggta aacataggcg gcgtttcgcc
      361 cggacataac tcggcgacca gatacccaac taacggtaag tgggagataa ccaacaccga
      421 agcaacacct tcattggtca acgcctgcaa ataggcactg accagaccaa catcgccgca
      481 gggcgttaac tccggcagaa cttctgcact ggaaggcagg ttcagacaat cccctacttc
      541 ctccagtgtt tgctcggctc gcaggaacgg gctcaccaga acgcgttcga tttccacttt
      601 ttgacctttc agccagttcg ccatcaggcg agattcgtca caaccattag tagtcagagg
      661 acgaaccgaa tcactggcgg catcgagggc tgcgtcgccg tgacgcatga taaaaacttg
      721 catattgcac cgcttttgtt aaccagattc gcccgttttc tttcatctga aaccagagaa
      781 agaaaacggt ggccggcatt gtgccttatc cattcaccga atgaaacgct gttttttacc
      841 tcaatggcgt aagtatagtc aatatgcgtt tacattttga ccaacaatcc gccattcagc
      901 gcggattcat atagctttga ccttcttatt gcaggtcagt tgcagttgtt ttccaaaaac
      961 tttccccacg cgcacccatc tcgactaaac gctcgcaagg ggtaaaacgt gaaccatact
     1021 gcgtggccag tcgttgcatt attgcaacca cttcgcccgc gccgagagaa tcgatatagc
     1081 ggaacggtcc accgagaaat ggcggaaaac caatgccaaa taccgcgcca atatccccgt
     1141 cacgcacgct acggataacc tgctcatcaa cacaacgtac tgcttcattc agcatcaaca
     1201 tcacacaccg ttcagcaacc tgcggtgcgg agagccgccc ctgcccttgt gcgccaatga
     1261 gcgggtaaat ggcaggatcg acctgttttt tgcttttacg ccctttctga ccataaagat
     1321 agaaaccccg accatttttt ctgcctttgc gatcgtcgtt caaaattgaa gaaacaacat
     1381 ttgcaggcgc gctaaaacgt tctccataag cggcttccag tacaggaata attttagtcc
     1441 cggtgtcgat tcctacctca tccaaaagtt ggattggacc taccggaaaa ccaaatttca
     1501 ctagcgcggc atcaatgtgc tctacccgtt caccttcggt cagcatgcgg atagcttcat
     1561 taatgtaagg cgctaagatg cgattgacgt aaaacccggc tttgtcacgc acgacaatgg
     1621 gcgttttgcc ctgttttttc gccagtttta ccgtagtggc gatggtttgc gctgatgtac
     1681 ccgcatgagg aataatctcc accagcggca ttttttccac cggactgaag aaatgcaggc
     1741 cgataacttg ctcaggtcgc gtggcgtgag cggcgatatc accaatcggt aaagatgacg
     1801 tattcgaagc aaagatggta tgagcggcgc aattttgctc aacttccgcc accatctgtt
     1861 gtttcaattc gagattttca aacaccgctt caataatcag atcgcgatgg gcaaagccgc
     1921 gatagtccgt cgttccggag attaatgcca gctgtttgtc acgttcgctg gctttgagat
     1981 ggcgacggcg cactttgcca tcaagctgat cccaactgta cttcagtgca tgatttatgc
     2041 cctgcgggtt gatatcttta attctgaccg gaagccccgc tttacaagca gtgacataag
     2101 caataccgcc gcccatcaag ccaccaccta aaattcccac gctgtttaat ggcgcaggcg
     2161 gcgcatcact accgggatct ttcttcactt ccgtactggc aaaaaagata ctacgcaaag
     2221 cttgcgattg tggcgtcatc gccagttcgc caaacgcccg tgcttcagcg tcatagccac
     2281 tgctggtgcc ctgcgctaat cccgtttcaa caacctccag gatgcgttct gtcgccggat
     2341 aattgccttg agttttgtgt tctgttttct tgccgaccat tttgaacagc agcgcacgac
     2401 ctaacggccc cgccagaata cgctcgcgta caggtagagg gcgggaagat gggcgatcct
     2461 tctttgccag ctcaacagcg gcttccagca gaatggagtg cggaacaacg tcatccacca
     2521 gccccagctt taatgcctgt ttcgcccgaa gttgttttcc ggtgaggatc atctctaatg
     2581 ctgtgctgac gcctatcaga cgcggtaaac gctgggtgcc gcctgaaccg ggtaacaatc
     2641 caagttgtac ttcaggcaaa ccgagcaccg ttttaggatc gtcagtacaa acgcgaccgt
     2701 ggcacgccag cgccaactcc agcccaccac ccaggcaagc gccatgaata gccgcgataa
     2761 ccggaatggg caaagcatga atctccgcca tcaactgttg gccctgccgc gccagagctt
     2821 ccgcttcttg cgccgttttg cagttgccga tcatgttgat gtctgcgcca gcaatgaagt
     2881 tgtccggttt agcggagaca aacaccacgc ctcgcaactc tttgttttca cggagttgct
     2941 taataatggc gcgcacctgc gaggcaaact ccgccttcag ggtattcatt ttctcacccg
     3001 gtacgtcgat ggtgatgata gcaatgttgt ccagacgaac attaagagta aacgctgatg
     3061 ccatttccat tattccgcct ccagaaccat tgccgcgcca agcccaccgg cagcacaggc
     3121 ggtaactaaa ccaaatccac cgccgcgacg gcgaagttca tgcaatgtct gggtaatcat
     3181 ccgcgcgccg gtcgccgcga agggatgccc gtatgcaatc gagccaccaa gcacgttaaa
     3241 tttgctatcg tccacttcac cggtggcatg cgcacgcccc agtacgtcgc gagcaaaacg
     3301 ttcgctcccc agtagctgaa tattggccaa cgtctgggcg gcaaaggctt cgtgcatatc
     3361 aatcaatgtc agatcgctca tcgtcaaacc ggcacgctcc agcgccagcg gtgttgacca
     3421 ggctggaccg agcaacatgt cctgccagac atcgatagcg gtaaatgcgt agctgcgcag
     3481 atatcccagc ggtaccagcc ctaattcttt cgcccgggat tcagtcatca ggatcaccgc
     3541 cgctgcgcca tcggtcagcg gcgtactgtt cgccgccgtt accgttccgt gtttacgatc
     3601 aaacgccgga cgcagctttg cgtaatcagc aagcgaggaa ttaccgcgaa tattgttgtc
     3661 ttccgcgagt agttgtttat aaggagggat aaaggcagtc atcacctctt ctttgagttt
     3721 tccgtctgac catgcctgag cggcacgctg atgcgaacgg tgcgctaatg catcttgctg
     3781 ttctcgggtg atgccgtagg ttttcgccat ttgctctgca gtgtcgccca tccgcaagcc
     3841 ggtagaatat tctgctaccg caggtggtac gggcattaag tcgcgcaaac gtaggcgaga
     3901 gaagagtttc agacgctggc tcattgtgcg ggctttgttg acatcaacca gcacgcgcgc
     3961 cagtttttta ctgacgccaa ttggcaatac cgaagaggaa tctgccccac cggcaatccc
     4021 cgctcggata gtccccgcca tcaggctttc tgcgacgttt gcaaccgcct ggaaactggt
     4081 agcgcaagcg cggctgacgc tgtaagcatc ggtatgcaca ttcattccag taccgagaac
     4141 aatttcacgc gcaatgttgg gagcttcagg catttgtacg acctgaccaa agaccagttg
     4201 ttcaatcact tcggcgggga tctcgctgcg tgccagcagt tcgcctacca ccatcttccc
     4261 taaatcaacc gcgggaatgc catgaaaagc cgtcgcctgg cgggcaaaag gcgtacgtaa
     4321 accgctaaca atggcgatac gatcgccctg gcgggtaacc agcggtaaaa cctgacccat
     4381 aacactcccc tgtaaaaaaa taaataaagt ggtctgacct gatcatagtc ttaaccattt
     4441 ttttacattt agccaagcgg agaaaaggga aagtgggagc tatgacacag agagaaaaga
     4501 agaagagaaa agaaaacgcc ctgccagcaa ttgacagggg cgtacgtacc cgagaggaat
     4561 taacgcagac ccagctggaa gatcagcatt tcagcttcgc aggcaaaagt gaaatcgata
     4621 tccaggcgca caccatcaga ctcttcagtg aaagtcgggg agattttgca cggttcggat
     4681 tccacgctac gggctttctc agtcagcgcc gccagcgttt gttctgcttc tgcgcggttt
     4741 gcaaacacgc ggctgtaaga cgcggtgcag tcggagttgt ccataatggt gccaacatcc
     4801 atacagcagc aaaccggggt ttcatcagca ctacatttac tcatcgttga tttcctctgt
     4861 atgtgcaccc aaggtgccag ataaacgttg tggatatttt acgcttccgg aaagtgctgc
     4921 tccagttgtt aattctgcaa aatcggataa gtgaccgaaa tcacacttaa aaatgatcta
     4981 aaacaaaatt cacccgaatc catgagtgcg ccacctccaa attttgccag ctggatcgcg
     5041 tttcttagat catatttgaa aaaagataga aacatacttg caacattcca gctggtccga
     5101 cctatactct cgccactggt ctgatttcta agatgtacct cagaccctac acttcgcgct
     5161 cctgttacag cacgtaacat agtttgtata aaaataaatc attgaggtta tggtcatgag
     5221 ccagaaaacc ctgtttacaa agtctgctct cgcagtcgca gtggcactta tctccaccca
     5281 ggcctggtcg gcaggctttc agttaaacga attttcttcc tctggcctgg gccgggctta
     5341 ttcaggggaa ggcgcaattg ccgatgatgc aggtaacgtc agccgtaacc ccgcattgat
     5401 tactatgttt gaccgcccga cattttctgc gggtgcggtt tatattgacc cggatgtaaa
     5461 tatcagcgga acatctccat ctggtcgtag cctgaaagcc gataatatcg cgcctacggc
     5521 atgggttccg aacatgcact ttgttgcacc gattaacgac caatttggtt ggggcgcttc
     5581 tattacctct aactatggcc tggcaacaga gtttaacgat acttatgcag gcggctctgt
     5641 cgggggtaca accgaccttg aaaccatgaa cctgaactta agcggtgcgt atcgcttaaa
     5701 taatgcatgg agctttggtc ttggtttcaa cgccgtctac gctcgcgcga aaattgaacg
     5761 tttcgcaggc gatctggggc agctggttgc tggtcagatt atgcaatctc ctgctggaca
     5821 aactcagcaa gggcaagcat tggcagctac cgccaacggt atcgacagta ataccaaaat
     5881 cgctcatctg aacggcaacc agtggggctt tggatggaac gccggtatcc tgtatgaact
     5941 ggataaaaat aaccgctatg cactgaccta ccgttctgaa gtgaaaattg acttcaaagg
     6001 taactacagc agcgatctta atcgtgcgtt taataactac ggcttgccaa ttcctactgc
     6061 gacaggtggc gcaacgcaat cgggttatct gacgctgaac ctgcctgaaa tgtgggaagt
     6121 gtcgggttat aaccgtgttg atccgcagtg ggcgattcac tatagcctgg cttacaccag
     6181 ctggagtcag ttccagcagc tgaaagcgac ctcaaccagt ggcgacacgc tgttccagaa
     6241 acatgaaggc tttaaagatg cttaccgcat cgcgttgggt accacttatt actacgatga
     6301 taactggacc ttccgtaccg gtatcgcctt tgatgacagc ccagttccgg cacagaatcg
     6361 ttctatctcc attccggacc aggaccgttt ctggctgagt gcaggtacga cttacgcgtt
     6421 taataaagat gcttcagtcg acgttggtgt ttcttatatg cacggtcaga gcgtgaaaat
     6481 taacgaaggc ccataccagt tcgagtctga aggtaaagcc tggctgttcg gtactaactt
     6541 taactacgcg ttctgataac gcgttcgcct ggataaagtc acctgctatg caggtgactt
     6601 taactccccc acttcaccga aagtagtttc ctcattgctt gcctcctgag ttttgcaaac
     6661 agcctgatgg cagcttgccc tttcaaatca gtaagtggta tccatagaca aacctgaagg
     6721 gaaacgcata ttttcaggcg ttctgctcgt ccttctcaaa gagtttactt ttctgaattt
     6781 ccaggatact caaccccctg gctattgt
//
LOCUS       DAZUCE010000254         6802 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZUCE010000254 DAZUCE010000000
VERSION     DAZUCE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.254"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..579)
                     /locus_tag="ACHY03_004435"
     CDS             complement(<1..579)
                     /locus_tag="ACHY03_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3907590.1"
                     /translation="MTLTQINSLLAVLDYGGFTEASKRLFMTQSAVSQAIAALEQELG
                     VNILLRDRRKAIQLTTAGHRIVQYLRAINREVNAVKEIAEQEKQNPQRTLRLGCFPSV
                     CACILPVVIRYFEIHHPNIKIIPFEENSTAIIDSLQNESIDAGFVHFPVSGMYSVPIY
                     QDKFTVVLPPNHALAKNSTITVEELMGEPLIIS"
     gene            731..1921
                     /locus_tag="ACHY03_004436"
     CDS             731..1921
                     /locus_tag="ACHY03_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3907591.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            2002..2652
                     /gene="inaA"
                     /locus_tag="ACHY03_004437"
     CDS             2002..2652
                     /gene="inaA"
                     /locus_tag="ACHY03_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFS3907592.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(2706..2960)
                     /gene="yfaE"
                     /locus_tag="ACHY03_004438"
     CDS             complement(2706..2960)
                     /gene="yfaE"
                     /locus_tag="ACHY03_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFS3907593.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(2960..4090)
                     /gene="nrdB"
                     /locus_tag="ACHY03_004439"
     CDS             complement(2960..4090)
                     /gene="nrdB"
                     /locus_tag="ACHY03_004439"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFS3907594.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(4324..6609)
                     /gene="nrdA"
                     /locus_tag="ACHY03_004440"
     CDS             complement(4324..6609)
                     /gene="nrdA"
                     /locus_tag="ACHY03_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFS3907595.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         1558 a         1662 c         1814 g         1768 t
ORIGIN      
        1 actaatgatt aatggttcac ccattaattc ttccactgta atagtgctgt tttttgccag
       61 agcatggttg ggcggtaaca ctacggtgaa tttatcctga taaatgggca cggagtacat
      121 tccgctcacc ggaaaatgaa caaaaccggc atcaatggat tcattctgaa gcgaatcaat
      181 aatcgctgtg ctattttcct caaaaggaat aatttttata ttcggatgat gaatttcaaa
      241 gtagcgaatc acaaccggta agatacaggc gcagacgctg gggaaacaac caagtctcag
      301 cgttctttga gggttctgct tttcctgctc ggctatctct tttactgcgt taacttcccg
      361 attaatggcg cgcagatatt gcacaatgcg gtgacctgct gtggtcagtt gtatagcttt
      421 acggcgatcg cgtaacagta tattcacacc aagttcttgt tcgagggcag caattgcctg
      481 acttaccgcc gactgcgtca tgaacaatcg cttgctggct tcagtaaagc caccgtaatc
      541 cagcacggcg agtaaagaat ttatttgggt aagagtcatt agagtttgct aatcaataat
      601 attaaaacga ttaatttctc taatagcatg ccgccattta tacttctgtt ctgcaaagta
      661 catcacaatt tgacgcaacc gcagtaacgg gagtgttgtc tgcgtgcgtc atcctgacag
      721 gcggtagggt atgaaagaga agttatggac gaaggatttt tgggcaataa ccatcatcag
      781 ctttattatt ttcttcgtct tttatgtttt actaacattg ttgccaattt atatctctga
      841 ccgcttgcat gcctctcctg ataaagcagg tttgttggtg actttatttt taattgcggc
      901 gattgttatt cgaccctttg ccgggcaatg ggtgggtaaa tattcgaata aaactattct
      961 ggtgctctct tctctggcct ttttggtggt cactgcgctg tatccttttt gccactcaat
     1021 agaatcactg ctttttatta gggtgcttca tggtattacc ttcggggtta tcacaacggt
     1081 aaagggaacg atttccgcgc ggctgatccc ggcctcccga cgtggggagg gcatcagttt
     1141 tttctctctg gcaatggggc tggcaatggt ggtcgggccg tggattggcc tgaatatggc
     1201 gcgctgggag gcctttaata tggctttctg gttatgtact ggcgttgcgg cggtggggat
     1261 tatcctgtcg ctgattatga ccgtgccgcc ggttatcagc catgccgacg gttcaaagcc
     1321 aaagatgggc ttcgccgcca tgttcgatcg cgcggcattg ccgtttgcca tggttacatt
     1381 ctttatgacc ttttcgtatg ccggggtttc tgcctttctg gcgctttacg cccgcgaact
     1441 taatctgatg tcggcggcca gtaatttcct gctctgctac gccatcttcc tgatgatctg
     1501 ccgtaccttc accggcaatg tttgcgacaa aaaaggcccg aaatatgtgg tttacccctg
     1561 cctgctgttc tttacggttg ggctggtggt tctcggctac acccagggca gcgtaatgat
     1621 ggtcgtttct ggcgcgttga ttggtatcgg gtatggttcc gtgacgccag tttttcagac
     1681 gcagattatc agttcagtgg aaccgcataa aatcggtgtc gcaaactccc tcttcttcaa
     1741 tgcgatggat gcaggcctgg cgctgggagc ctgtgtgatg gggatgatgg ttgcacatac
     1801 tggctaccga atgatttatc tgctgggcgc actattagtg gtagtggctg gtggagtcta
     1861 tgcgctgcaa atgaagggta aaagcggtgt cgcgctagta gtggcaaaag aaattcatta
     1921 atacgacacg tttcattaag atttttcgca ggtaaccacc tatagtcatt gtcgttaata
     1981 aaaaatggca ggcgataggg tatggcagtt tcagcaaagc acgacgaatt taatcactgg
     2041 tgggcaacag agggcgactg ggttgaagag cctaactatc gccgtaatgg tatgagcggg
     2101 gtgcaatgcg tcgagcgcaa cggcaaaaag ctatatgtaa agcgcatgac gcatcatctg
     2161 tttcattccg tacgttatcc tttcggccga ccaacgattg tccgtgaggt tgcggtaatt
     2221 aaagaactgg aacgggccgg tgtcatcgtg ccgaagatcg tttttggtga agcggtgaaa
     2281 attgaggggg aatggcgcgc gttgctggtg actgaagata tggcggggtt catcagcatt
     2341 gctgactggt atgcccagca tgcagtaacg ccctattctg acgaagtacg gcaagccatg
     2401 ttgaaagcag tggcgctggc gtttaagaaa atgcatagcg ttaatcgtca gcatggctgt
     2461 tgttatgttc gccatatcta tgtgaaaaca gaaggtaagg cagaagctgg ttttcttgat
     2521 ctggagaaaa gtcgtcgccg cttgcgtcgc gataaagcga ttaatcatga cttccgccag
     2581 ctggaaaaat atctggagcc gatccccaaa gcggactggg agcaggtaaa agcgtattac
     2641 tacgccatgt aatcgttgta ggccagataa tacgcgtaag cgtcgtatct ggcgctagta
     2701 cccgatcaca actcgatttc aatatcgcct tttgcccggc aacaacaggg caaaatttcc
     2761 cccggctgaa taaaggctaa cggttcggca atccagtcca cctggcctgc aacaaggcgc
     2821 gtgcgacagg agccgcagta accttcgcgg cactggtact caaccgccac attgtgggat
     2881 tccagcgccg ccagaaggga agggtgttca tcctggcaca gcagttgtgt gccagtgatg
     2941 cgcagggtaa cgcgggccat cagagctgga agttactcaa atcgtcggtg tccacttccg
     3001 cgtcaatctg cccgaccaga taagaactga cttccacttc ctgcggagca acctgcacgt
     3061 tatcagacac cagccaggtg ttgatccacg ggatcgggtt ggagcgcgtc tggaacggca
     3121 gatccaaacc gactgcctgc atacggatat tggtgatgta ttcaacgtac tggcagagaa
     3181 tatctttatt cagaccaatc atcgaaccgt cacggaacag ataatccgcc cagtctttct
     3241 cctgttgtgc cgcctggaca aacaggtcat agcactcctg cttacattct tcggcaattt
     3301 ccgccatctc cggatcatcc gcgccgctgc gcagcagatt cagcatatgc tgggtgccgg
     3361 tcaggtgcag ggcttcatcg cgggcaatca ggcgaataat cttggcgtta ccttccatca
     3421 gttcgcgttc tgcaaaggca aaggaacaag caaagctgac gtagaaacga atcgcttcca
     3481 gcgcgttaac gctcatcagg cagagataca gttttttctt cagctcgcgc aggctaacgg
     3541 ttacggtttt accgttaacg gtgtgggtgc cttcgcccag cagatgccag tagctggtca
     3601 tttcgatcag ctcatcgtaa tagctggaga tcccttccgc acgtttctgg atctgctcgt
     3661 tggtgacgat atcgtcaaac acaacagacg gatcgttaac gatattacga atgatatgag
     3721 tataggaacg ggaatgaatc gtttctgaga acgcccaggt ttctacccag gtttccagtt
     3781 ccgggatcga gatcagcggc agcagtgcca cgttcgggct acgaccctga atggaatcca
     3841 gcagcgtctg atatttcagg ttgctgataa agatgtgttt ttcgtgctcc ggcagcgcct
     3901 ggtaatcgat acggtcgcgg gagacgtcaa cttcttccgg acgccagaag aaagagagct
     3961 gcttttcgat cagcttttcg aagatgtcat atttttgctg atcgtagcga gccacgttga
     4021 ccggctgacc aaaaaacatc ggttctttga gctgatcatt tttcgtctgt gaaaaggtgg
     4081 tatatgccat gagtgtgtcc tgttgggagt ttaatgccgg ataaggcatt ttacgccgca
     4141 tcctgtgcct gatgcgacgc tggcgcgtct tatcaggcct acaaatccga gccgtaggcc
     4201 ggataaggcg ttcacgctgc atccggcacc cggagcctga ggcgacgctg gcgcgtctta
     4261 tcaggcctac aaaccgagcc gtaggccgga taaggcgttt acgccgcatc cggcatctca
     4321 atatcagatc ttacatgcgc cgctttcgca gccatcgtcc tggattgacg gcaccagatc
     4381 gtcctgcgcg tcttcagcgc cgtcacgggt gttctgataa tacagcgttt tgaccccgaa
     4441 tttgtaggcg gtgagcaggt ctttcagcaa ttgctgcatc ggcacttttc ctgacgggaa
     4501 gcgtgacgga tcgtagttgg tgttagcaga gatcgactga tcgataaatt tctgcatgat
     4561 acccaccagt tgcaggtagc catcgttacc cggcatttcc cacagcagct cataggcgtc
     4621 gtgcagatgc tcgtagtccg gcaccacctg gcgcagaata ccgtctttcg acgctttgat
     4681 gctgacgtaa ccgcgcggcg gttcaatacc gttagtggcg ttagagatct gcgaagaagt
     4741 ctcggacggc atcagagcag aaagcgtgga gttacgcagg ccgtgcgttt tgattgactc
     4801 acgcagagct tcccagtcgt aatgcagcgg ctcattagcg atggtatcca gatctttctt
     4861 ataggtatcg atcggcagga tccctttcgc gtaagtggtt tcgttaaacc acgggcacgc
     4921 gccttgctct ttcgccagct cattagacgc tttcagcagg taatactgaa tcgcttcgaa
     4981 ggttttatgc gtcaggttgt tggcgctgcc gtcggagtag cgtttaccat gcttcgccag
     5041 gtagtaagcg aagttgatca caccaatacc cagcgtacga cgacccatcg ctccacgttt
     5101 ggcggccggg atcgggtaat cctgataatc cagcagcgcg tcaagtgcac gaaccgccag
     5161 aattgccagc tcttccagtt catccaggct attaattgcg cccaggttga aagcagacag
     5221 cgtacacagc gcgatttcac cgttctcgtc gttgacgtcg ttcagcggtt tggtcggcag
     5281 ggctatctcc aggcacaggt tagactgacg cactggcgcg atggccggat caaacgggct
     5341 atgggtattg cagtggtcaa cgttctgaat atagatacga ccggtagatg cacgttcctg
     5401 catcatcagc gagaacagct caacggcttt cacacgctgc ttgcggatgc tgtcgtcttt
     5461 ctcatatttg gtatacaggc gttcaaactc ttcctgatcg gcgaagaatg cgtcgtacag
     5521 ccccggtacg tcggacgggc tgaacagggt gatatcttca cctttcagca gacgggtata
     5581 catcagtttg ttgatttgta ccccgtagtc catatgacgc acgcggttgc cttccacacc
     5641 acggttgttt ttcaacacca gcaggctttc cacttccaga tgccacatcg ggtagaacag
     5701 cgttgccgca ccgccgcgca caccgccctg agagcaggat ttcaccgctg tctggaaatg
     5761 tttgtagaac ggaatacaac cggtatggaa cgcttcgcca ccgcgaatcg ggctacccag
     5821 cgcacgaata cgcccggcgt tgatgccgat cccggcacgc tgggatacgt atttaacaat
     5881 cgcgctggag gtggcgttga tggagtccag gctgtcgccg cactcgatca gtacgcagga
     5941 gctgaactga cgagtcgggg tacgcacgcc ggacatgatt ggcgtcggca gcgaaatttt
     6001 aaatgtggaa accgcgtcgt aaaaacgctt cacgtattgc aggcgcgttt cacgcgggta
     6061 gttcgagaac aggcacgcag caaccagaat ataaaggaac tgggcgcttt cgtagatttc
     6121 gccggtcacg cggttctgta ccagatattt gccttccagt tgcttaacgg cagcataaga
     6181 gaaggtcata tcacggtcgt gatcgataaa cgtgtccatc tgcttgaact cttcttccgt
     6241 gtagtcttcc agcagatgat tatcgtattt gcccatctcg accattttca ccacgtggtc
     6301 gtacagcgca ggcggctcaa actggccgta ggctttttta cgcaggtgga agatcgccag
     6361 gcgcgcggcg agatactgat aatccggcgc atcacgggag atcaggtctg cggcagcctt
     6421 gataatagtt tcgtggatgt cagaggtctt gataccgtca taaaactgaa tgtgggagcg
     6481 cagctcgacc tgggaaatcg aaacgttatg cagtccttct gccgcccaat ccagaacgcg
     6541 atggattttg tcgagattga tgcgctctgt gctaccgtcg cgctttgtca ccagcagatt
     6601 ctgattcatg tatgtcgtac ctgtttttgg aattctttcc gcaatagttt cgtgcagact
     6661 acctgtagta cgggaagctg cttagaacac tatatatagg gggtatgttt gggattcact
     6721 gcaagatagt gtgaaaatgg ccctcttgca agtgcataac tttgtggata actcaggaag
     6781 gaaaaagtgg ctttcgcgca cc
//
LOCUS       DAZUCE010000255         6597 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZUCE010000255 DAZUCE010000000
VERSION     DAZUCE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.255"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(184..939)
                     /gene="mlaA"
                     /locus_tag="ACHY03_004441"
     CDS             complement(184..939)
                     /gene="mlaA"
                     /locus_tag="ACHY03_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFS3907596.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1233..2165
                     /gene="yfdC"
                     /locus_tag="ACHY03_004442"
     CDS             1233..2165
                     /gene="yfdC"
                     /locus_tag="ACHY03_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFS3907597.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNNKNPA"
     gene            2241..2315
                     /locus_tag="ACHY03_004443"
     tRNA            2241..2315
                     /locus_tag="ACHY03_004443"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2274..2276,aa:Arg,seq:cct)
     gene            2477..3634
                     /gene="intS"
                     /locus_tag="ACHY03_004444"
     CDS             2477..3634
                     /gene="intS"
                     /locus_tag="ACHY03_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFS3907598.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYANELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPEFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENKIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            4007..4960
                     /gene="ompT"
                     /locus_tag="ACHY03_004445"
     CDS             4007..4960
                     /gene="ompT"
                     /locus_tag="ACHY03_004445"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFS3907599.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            5147..>6597
                     /locus_tag="ACHY03_004446"
     CDS             5147..>6597
                     /locus_tag="ACHY03_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3907600.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKTQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKPENI"
BASE COUNT         1984 a         1493 c         1478 g         1642 t
ORIGIN      
        1 aaaattattt ttccagtttt ttgatttttc aacgactgaa tatctgaaat agtcaatgac
       61 gaacatcagt atttttacat cattacgtcc gggtcattgt cggtttatct ccttttactt
      121 gtggtaattg agggataaca acaaaaaagg tgagttttgc gactcacctt ttttatttgt
      181 ttcttattca gaatcgatat cttttaaatc atcctgaatc gcttgcgcgt tcggattttc
      241 ctgcggtttg agttcgccgc cattagcgat gaaatcatga cgctggaagt acgcttcgcg
      301 caccataatg taaggatcgg acgactgacg cagcagaccg tcggaatcca gcagctgtgc
      361 gcgggtttcg ataccttcaa gcgtccattt acccacagac atcggccagg tcaaccagga
      421 aagaaccggg taaagaccat ccgccatatc accaccgtca tcacgcagcg tgaagctacc
      481 gtagaacggt aattgaacgt acgggccata acccacgcca taatgaccaa gcgtactacc
      541 gaagcggtga ggttcagtcc gttgcagttt cgggttcgcc atccctgcaa catcaataaa
      601 accacccatc cccaaaatgg tgttcaggaa aaagcgggta aagtggacca tcccctgata
      661 agggtcgccc tgcaagaagt agttaaccat caccgcaggt tcttcaaggt tgccggtaaa
      721 gttgctcaaa ccgttacgcg ccggttgtgg aacataatca cgccaggcaa cagcgaccgg
      781 tcgaacaata tacgggtcta atacattgaa gttgaagttg tacatggtgc ggttgaaccc
      841 ttctaacggg tcagaacgcc cttgctgatc tgtaccggaa ctcgcacacc ccaccagaag
      901 cgtagttccc agagcaagcg ccgacaggcg aagcttcata aatgtctccc tgttttttta
      961 tggcttatgc agtttgccat ccatgacgga acgataccgt atccgcctgt ttaggtgtgg
     1021 gcgattgtaa cagcacgtca actgatgtcc agacgccctg atttgctgat ttgatcatag
     1081 cctggtaatc gccgccctgt aggctacttg attctatgga aacagaaaaa ggcaaacgcg
     1141 gccttttcta cattttcaga gtaactcccg ccagttgcga gcaaaaaagc cgctacgctt
     1201 tagctatacg tgctaattca agagaagaga ccatggacaa cgacaaaatt gatcaacaca
     1261 gcgacgaaat tgaagttgag agcgaagaaa aagagcgtgg caaaaaaata gaaatagatg
     1321 aagatcgact cccctcccgg gcgatggcaa ttcatgagca tatccgccag gatggtgaaa
     1381 aagagctgga acgcgacgca atggcactac tgtggtcagc cattgcggcg ggtctgtcga
     1441 tgggcgcttc attactggca aaagggatat ttcatgtcga actggaaggt gtgccgggca
     1501 gcttcttgct ggagaatctc ggttatacct ttggttttat tatcgtcatt atggcccgcc
     1561 agcaattatt taccgaaaac accgtgactg cggtactacc cgtcatgcaa aaaccgacaa
     1621 tgagcaacgt tggcttactt atgcggttat ggggcgtcgt gctgctgggt aatattctcg
     1681 ggacaggtat tgcggcgtgg gcatttgaat atatgcctat cttcaatgaa gaaactcgcg
     1741 atgcatttgt caaaatcggc atggatgtga tgaagaacac ccccagcgag atgtttgcca
     1801 acgcgatcat ttccggctgg ctgatcgcca ctatggtatg gatgtttcct gcagcgggtg
     1861 cggcaaagat tgtggtgatt atattgatga cctggcttat tgccctgggt gacaccaccc
     1921 acatcgttgt cggttctgtt gaaatcctct atctggtgtt taacggtacg ctgcactgga
     1981 gcgatttcat ctggcccttc gcactaccta ctttagcggg gaacatctgc ggcggcacct
     2041 ttatcttcgc gttaatgagt catgcacaga tccgtaacga catgagcaac aagcgcaaag
     2101 cagaagcacg ccaaaaagca gaacgtgcgg aaaacattaa gaaaaataat aaaaacccgg
     2161 cataaatggc gagggtttaa gcaatcgagc ggcagcgtac ttaccccgca gtccattagc
     2221 gggtatactc atgccgcatt gtcctcttag ttaaatggat ataacgagcc cctcctaagg
     2281 gctaattgca ggttcgattc ctgcagggga caccatttat cagttcgctc ccatccgtac
     2341 cagtccgcaa aatcccctta atatcaagcc ttccgtagat tcacagttcg tcatggttcg
     2401 cttcagatcg ttgacagccg cactccatga cgggtaaaaa gtggataaaa taattttacc
     2461 caccggattt ttacccatgc tcaccgttaa gcagattgaa gcagcaaagc cgaaagaaaa
     2521 accataccgc cttctcgatg gtaatggcct gtacctttat gtccctgtat ccgggaaaaa
     2581 ggtatggcag cttcgctaca agattgacgg taaggaaaaa atactgaccg taggaaaata
     2641 tccgcttatg actttgcagg aggcaaggga taaagcatgg actgcgagga aagacatctc
     2701 ggttggcatc gatccggtaa aagcgaaaaa ggcttcgtct aacaacaact cctttagcgc
     2761 catttacaag gaatggtacg agcacaagaa gcaagtctgg tcagtaggct atgcaaatga
     2821 acttgcaaaa atgtttgatg acgacatttt acccatcatc ggcggccttg aaattcagga
     2881 tattgagccg atgcaactgt tggaagtaat ccgcagattt gaagatcgcg gcgcaatgga
     2941 gcgagccaac aaagcacgca gaagatgcgg cgaggttttc cgttacgcta ttgtcaccgg
     3001 aagggctaaa tataacccgg cacctgacct tgctgacgcc atgaagggat accgcaagaa
     3061 gaacttcccg tttcttcctg cagaccagat cccggaattc aacaaagcac tggcaacatt
     3121 ttcaggaagt atcgtatcgc tcattgccac caaggtttta cgctacacag ccctaagaac
     3181 gaaagagctt cgttccatgc aatggaagaa cgtcgatttt gaaaacaaga ttatcaccat
     3241 cgacgccagt gtgatgaagg gacgcaagat tcatgtggtc ccgatgtcgg accaggtggt
     3301 tgaacttctc actacgctaa gctccatcac taaaccagta tcagagtttg tttttgccgg
     3361 acgcaacgat aagaagaagc caatctgcga gaacgcggtg ctacttgtga tcaaacaaat
     3421 cggctatgag ggtctggaaa gcggtcacgg attcaggcat gaattcagca cgattatgaa
     3481 cgagcacgaa tggcctgctg atgctattga agtgcaactg gcacatgcca acggcggatc
     3541 tgtgcgcggg atttacaacc atgctcagta tctcgataag cgcagagaaa tgatgcaatg
     3601 gtgggcggac tggcttgatg ggaaggtgga gtaatccgcc ttaaccacta tcgaagagca
     3661 caaagccttg caatccagta caaagctatg tgtgtctcag ttttgtccct cttttttgta
     3721 ctaaaaaaca tagcaattga ggataaacct catgctattt tcgcttatat gcctttaaag
     3781 gcatggcgct taaatagata aaaacaccac aaaagcataa aaaaccacac agtaaaaccg
     3841 caacataaaa caataacaga taattaaacc aaaaacagat agcgcattgt gataatcatt
     3901 caatactaaa caaaatataa acagtggagc aatatgtgat tgactcatta agttagatat
     3961 aaaaaataca tattcaatca ttaaaacgat tgaatggaga acttttatgc gggcgaaact
     4021 tctgggaata gttctgacaa cccctattgc gatcagctct tttgcttcta ccgagactat
     4081 atcgtttact cctgacaaca taaatgcgga cattagtctt ggaactctga gcggaaaaac
     4141 aaaagagcgc gtttatctag ctgaagaagg aggccgaaaa gtcagtcaac tcgactggaa
     4201 attcaataac gctgcaatta ttaaaggtgc aattaattgg gatttgatgc cccagatatc
     4261 tattggggct gcgggctgga ctactctcgg cagccgaggt ggcaatatgg tcgatcagga
     4321 ctggatggat tccagtaacc ccggaacctg gacagatgaa agtaggcacc ctgatacaca
     4381 actcaattat gccaacgaat ttgatctgaa tatcaaaggc tggctcctca acgaacccaa
     4441 ttaccgcctg ggactcatgg ccggatatca ggaaagccgt tatagcttta cagccagagg
     4501 tggttcctat atctacagtt ctgaggaggg attcagagat gatatcggct ccttcccgaa
     4561 tggagaaaga gcaatcggct acaaacaacg ttttaaaatg ccctacattg gcttgactgg
     4621 aagttatcgt tatgaagatt ttgagctagg tggcacattt aaatacagcg gctgggtgga
     4681 agcatctgat aacgatgagc actatgaccc aggaaaaaga atcacttatc gcagtaaagt
     4741 caaagaccaa aattactatt ctgttgcagt caatgcaggt tattacgtaa cacctaacgc
     4801 aaaggtttat gttgaaggcg catggaatcg ggttacgaat aaaaaaggta atacttcact
     4861 ttatgatcac aatgataaca cttcagacta cagcaaaaat ggtgcaggca tagaaaacta
     4921 taacttcatc actactgctg gtcttaagta caccttttaa cgaagttaac cagattttct
     4981 ccccggtggt attgtataac cccggggaat ttgtttacag ttacaagtag gcaatatcca
     5041 ctaattttgt aagcatttaa cctatgtcat aacgtgtaat ctcttaccgc aacacaagac
     5101 ctcaatatat tcaaatctta tctttattcc gatattcaga gacacaatgc gaaaaataat
     5161 tactcatttc aaagttgttt taacgttact tctaccagta accgtatctg cccagcagat
     5221 acagtggcaa tcctgtatgg ccagtcaatt caaccactgg tttggtgagg aaaaaccgtc
     5281 tcctgactta ctatgtggtt atttgtctgt tccattaaaa tatacagaca caggcggaga
     5341 tgcttcttat gaaaaaaaaa cacaagtcaa actagcgttg acaaaattgc cggcaaaaag
     5401 caagcataaa ggaagtatcc tgataataag tggtggtccc gggttaccag gcataaatcc
     5461 ttatattaac tttgactggc cagtcacaaa tcttcgtgag tcatgggata ttattggatt
     5521 tgatcctcga ggcgtcggac agtccactcc gacaataaac tgccggcaat cagatacaga
     5581 gactcaggaa aacataaccg aaaagcaaca agtattaaat aaaattaatg cctgtatcca
     5641 taataccgga gccgaagtca ttcgccatat cggctctaac gaggctgtat acgatattga
     5701 tcgtattagg caagccttgg gggataaaca actgacagcc gtggcgtatt cgtatggaac
     5761 tcaaattgca gccttatatg cagaacgttt tccctacaac gtaagatcta tcgttcttga
     5821 tggagtcgtc gatatcgatg acctggagga caacttcaca tggcaactca aacaggcaca
     5881 gagttatcag gaaacgtttg atcgctttgc atcctggtgt gcgcgtacaa aaagttgccc
     5941 gctttcttca gacagagata aggcaataac tcagttccat gagctattat caaaattaca
     6001 tcacaaacct ttattagaca gtaagggaga aaatatatct tcagatgaac tcatatcatt
     6061 aacaacagac cttctgctat ggcgttcatc atggccaacc cttgcaactg ccatacgcca
     6121 gttctctcag gggattgtca gtaatgaaat tgaaactgcg ctcagtgctc cgatagcctc
     6181 agaagagtca agcgatgctt cgggggtaat cctctgtgta gatcaggggg atgagcaatt
     6241 aacaccagaa gagcgaaaat cccgaaaaga cgctcttgcg aatgccttcc cggctattaa
     6301 ctttgacaat ggacgttccg attcacctga tttttgtgaa ttatggccaa tacatagcga
     6361 cctgaacaaa actcgcctga aaaatactgt tctgccctct ggtttactgt ttgtagcaca
     6421 caaatacgac ccaacaacgc cctggattaa tgcccgtaag atggcagaga aattttccag
     6481 cccgttacta acaataaatg gtgatgggca tacattagct ctcaccggag ttaatttatg
     6541 tgtagataaa gcagttgtac atcacctgat cactccacaa aaaccagaaa atatata
//
LOCUS       DAZUCE010000256         6591 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZUCE010000256 DAZUCE010000000
VERSION     DAZUCE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.256"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1637
                     /gene="espL4"
                     /locus_tag="ACHY03_004447"
     CDS             <1..1637
                     /gene="espL4"
                     /locus_tag="ACHY03_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFS3907601.1"
                     /translation="FLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYFDKME
                     SNGISVMAVILLVDNHALTARLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMSESKEDI
                     KHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDEITPPLSPATM
                     NLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSINLPGLYLAINYGNADIVETIF
                     NSLSETGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSILNALPKLAATH
                     HLDNEQVYKFLSAKNSTSSHVLYHVMANGDADMLKIVLNALPLLIRTCHLTKEQVLDL
                     LKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISASDIVDLLTAKSLARDT
                     GLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQ
                     QNVVETVYLALSDHARLFGFTAEDIMGFWQHKAPQKYSAFELACELGHRVIAELIFNT
                     LNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1606..3342)
                     /gene="aceK"
                     /locus_tag="ACHY03_004448"
     CDS             complement(1606..3342)
                     /gene="aceK"
                     /locus_tag="ACHY03_004448"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFS3907602.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRTLQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3526..4830)
                     /gene="aceA"
                     /locus_tag="ACHY03_004449"
     CDS             complement(3526..4830)
                     /gene="aceA"
                     /locus_tag="ACHY03_004449"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFS3907603.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAEAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4860..6461)
                     /gene="aceB"
                     /locus_tag="ACHY03_004450"
     CDS             complement(4860..6461)
                     /gene="aceB"
                     /locus_tag="ACHY03_004450"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFS3907604.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1652 a         1653 c         1607 g         1679 t
ORIGIN      
        1 aatttctcac tcacttcaac aaaaaatgca aaaaccagac tttggcgttg atttccagcc
       61 gtcctgaggg gcgttgcgtt gctgcctgcg gtgatttcgg gctagttatg aaggcatatt
      121 ttgacaagat ggaatcaaat ggcatcagtg ttatggctgt catattattg gtagataacc
      181 atgctttgac ggcccgacta agaataaaga acacaactga aggatgtacc cattacgtgg
      241 tttcggttta tgatccaaat gtaactaacg ataaaataag aatcatgagc gaaagtaaag
      301 aggatattaa acactattct ctgatggatt ttatgaatgt agattatagc ctcctgaaat
      361 ggtcaaatga tcatgttatt aaccaatctg ttgcaataat tccagcactt ccgaaagaac
      421 agctattgat gttaaaagga tctgtggatg aaataacccc tccattatca cctgcaacga
      481 tgaatttgct aatggcaatt ggtcagaatc accaacttac acaactgatg attcaactcc
      541 agaaaatgcc agaactccat agaacagaaa tgttgactgc ctataatagt attaacctgc
      601 caggtttata tttggctata aattatggta atgcggatat cgttgagact attttcaatt
      661 cattgtcaga aacaggatat gaagggttac tctcgaaaaa aaatctcatg catattctgg
      721 aggcaaaaga taaaaatggt ttttctggat tatttttagc gatatcacgt aaggataaaa
      781 atgttgtaac ctcgattctg aacgccttac ctaaactggc cgcaacacat catttagata
      841 acgaacaagt atataaattc ctgagtgcca aaaatagtac gtccagccat gttttatacc
      901 atgttatggc gaatggtgat gccgacatgc tgaaaattgt tttgaacgcg ttacctttgt
      961 taattcgcac atgtcatttg actaaagaac aggtactcga tctcctgaag gcaaaggatt
     1021 tttatggttg cccaggacta tacctggcga tgcaaaatgg acatagcgat atcgtgaaag
     1081 ttattcttga agcattgccc agcctggccc aggaaattaa catttcagct tccgatattg
     1141 tagatcttct gaccgctaaa agtcttgcgc gcgacacggg tttgtttatg gccatgcagc
     1201 gcggacatat gaacgttatt aatactattt ttaacgcatt acccactctg tttaacacgt
     1261 ttaaattcga taaaaaaaat atgaagcccc tcctcctggc aaataattct aatgaatatc
     1321 ccggtttgtt ttcagcgata cagcataaac aacaaaacgt tgtagagacg gtttatcttg
     1381 ctttatctga ccatgcacgc ctgtttggat ttaccgctga agatattatg ggtttttggc
     1441 aacacaaagc gccacaaaaa tattctgcct ttgagttggc ttgtgaattg ggtcaccggg
     1501 ttattgctga attaatcttt aatacattaa ataagatggc tgaaagcttt ggctttacgg
     1561 ataaccctcg atacattgcg gagaaaaatt atatggaagc tttactcaaa aaagcatctc
     1621 cccataccgt acgctaaatc tttgcctgcg ccgatacgca taaacatctt ccacatgtcc
     1681 ttcacgaatg cggttttgta gtgtgcgcca gtaatcagcg cggaacaggt cggcgtgcat
     1741 ctcttcaaag agtggcccaa tgcgggggtc ggcgcatagc cagtggcgga actcttccgg
     1801 gaaaacatcg cccggcgaga cgctgtacca cggttcgctg gcaagttcgt cttccggata
     1861 gcgcggcggc gggatgtcgc ggaagttcac ttccgtcatg tagcaaattt catcgtaatc
     1921 ataaaaaacc acacgcccgt gacgggtgac accgaagttt ttaaacagca tgtcgccagg
     1981 gaaaatgtta gcggcagcaa gctggcgaat ggcgttgccg tactcttcaa tagcgtcgcg
     2041 cagttgctgg ccctcaacct gttccagcca gatattgagc ggcaccatcc gccgctcaat
     2101 atagagatgg cgaatcacaa tctgttcgcc gagatcggtg attttttccg ctgcttcctg
     2161 gagcagtaat tccattaatg ccggggaaat atgccgcttc tccagcacaa agttttcaaa
     2221 ctcctgggtg tccgccattc ggcccacgcg atcgtgctct ttcaccagtt gatagcaggc
     2281 acgaacgtga gcggcagaca tctctttctg cggcgcgaac ttgtctttga tgactttaaa
     2341 tacacggtca aagcccggca gcgtgaacac caacatcacc atgccacgaa tacccggcgc
     2401 ttcaatgaac tgctcattac agccctgtag ataaacgaga tattcgcggt agctttcggt
     2461 tttggcgtgc ttctggcagc cgatagccat atacaattca gcggtggttt tacctggcag
     2521 aatttcccgt agccactcga ccagcgctgc gggcagcggt gcataaacca taaaataaga
     2581 acgcgcaaag ccaaaaacaa tgctcgcttc ggcggtcgtc gtcaggcagg tatcaataaa
     2641 taactcgccg tcgtccgtct ggtggatcgg cagcaaaaat ggcaatgtgc cggaaggcgt
     2701 gatcagtttg cctaccagcc aggcggcttt attgcggtaa aacagttcgt tcgccacctg
     2761 taaatgactt ttggagaggt tgtctgtccc caacgtttcc gtcagatggc gaataatgta
     2821 atggatatca cggcttttat tctgccagcg caggcgcagc ggcaggtcgc tgataacgcg
     2881 catcagtaga gattcccagc cgtgatcggg gtgaaagtct ttcgccagcg ggcggggaat
     2941 ggtacgaaag cggcgctctg gctgagagct aaagataaaa agccgctcgg gagtaagcga
     3001 gcggtggtca aataaccgac agtacacgga gttaaaaaag ctctccgcaa tctcgaagcg
     3061 cgggtaatcc ggcaacagcc gggtgtaatg ctctttgacg cgcagtaaaa attccgcgtc
     3121 cgtactttgg ccgttagtaa tgcagcgcag ttgctccacg accagaccaa cgtgatgatc
     3181 gtaaagatgg atacggtttt tcatcgcctg ctggacagca tgccagtcgg cctgttcgaa
     3241 acgctgctgc gccccggaag tcacttcgag gaatcgacca tactgagcgt cgaagccttg
     3301 caaaatggtt tgagcaatca gtaattccag gccacgcggc atttagcgcc ctcatcagga
     3361 gcagagaatt gccggatgcg gcgtaaacgc cttatccagc ctacgttcgg caacggttgt
     3421 aggcatgata agacgcgcaa gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc
     3481 gtaaatgcct tatccggcct acagtcagca acgtttgtag gttgcttaga actgcgattc
     3541 ttcagtggag ccggtcagcg cggtgactga agacgtgccg ccctgaataa tagtcgtcac
     3601 tttatcgaag taacctgtac ccacttcctg ctggtgagat acgaaggtgt aaccatcttt
     3661 cgcggcggcg aattccggtt gctgcacttt ctcaacgtag tgcttcatac cctcgccctg
     3721 ggcataagcg tttgccaggt caaacatgtt gaaccacatg ctgtggatac ctgccagggt
     3781 gatgaactgg aacttgtagc ccatatccga cagctgctgc tggaagctgg caatagtgtt
     3841 gtcgtcgagg tttttctgcc agttgaacga cggcgagcag ttataagcca gcagtttgcc
     3901 cggatatttc gcgtggatag cttccgcaaa gcgacgcgcc agttccaggt ctggcgtgga
     3961 ggtttcacac cataccaggt cagcatatgg cgcatacgcc aggccacggc tgatcgcttg
     4021 ctcaatgccc gcatgagtac ggaagaagcc ttcactggta cgctcgccgg taataaattc
     4081 gctgtcatac gggtcgcaat cggaggtaat cagatccgcc gcatcggcat cggtacgagc
     4141 aaccagtagg gttggaacgc ccgtcacgtc agctgccaga cgcgccgcga ccagtttctg
     4201 aatagcttct tgagttggca ccaaaacttt gccgcccatg tgaccgcatt tcttcactga
     4261 cgccagctga tcttcgaagt gaactgccgc tgcaccggct tcaatcatcg ctttcatcag
     4321 ttcaaaggca ttcaggacac cgccaaaacc ggcttccgca tcggcaacga tcggcaggaa
     4381 gtaatcgaca tagcgcggat cgcccggctc aatgcctgcg gaccactgga tctgatccgc
     4441 acgacggaag gtgttgttga tccgctccac cacggctggc accgagtttg ccggataaag
     4501 cgactggtcc gggtacatgc ttgccgccaa gttagcgtcc gccgctacct gccatcccga
     4561 cagatagacc gcttcaatac ccgctttcgc ctgttgcagc gcctgaccgc cagtcagtgc
     4621 accgaggctg ttgatgtagc cttttttcga ctcgccatgc agaagacgcc acattttcgc
     4681 cgcgcccagt tgcgccagcg tgcattcagg attgactgaa ccgcgtaatt tcaccacatc
     4741 ttccgcgctg tatgggcgag taatgccttc ccaacgcggt tgagtccact ctttctgtaa
     4801 ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc tccatagtta tgtggtggtt
     4861 tacgctaaca ggcggtagcc tggcagggtc aggaaatcaa ttaactcatc ggaagtggtg
     4921 atctgttcca tcaagcgtgc ggcatcgtca aaacgcccct gggagaaacg ttcttcgccc
     4981 agttcgctgg caatgacttt catctcttcg cccagcatct ggcggaacaa ggctttggtc
     5041 accggtttgc cattgctcaa cgttttttga tgatggatcc actgccagat tgaggtacgg
     5101 gaaatttcag ccgtcgccgc atcttccatc aggccataaa tcggcacgca gccgttgccg
     5161 gagatccacg cttcaatgta ctgcacagcc acgcgaatgt tggcgcgcat accttcttcg
     5221 gtgcgttcac catcacaagg tgccagcagc tgatcggcag taatcggcgc gtcttgttcg
     5281 cgcatcactt caagctgatt tttacgggag ccgagaatgt cgttgaatac cgccattgcc
     5341 gtgtccgcaa ggcctgggtg agcgatccat gtgccatcgt gaccgttatt ggcttccagc
     5401 gatttatccg cttttacttt gttgagcacc tggttattgc gctcttcatc tttgctcgga
     5461 ataaacgccg ccatgccgcc catcgcaaaa gcaccgcgtt tatggcaggt tttaatcaac
     5521 aggcgtgagt aagcattcag gaatggttta tccatcgtca ctgcctgtct gtctggcagg
     5581 acgcgatcgg gatagttttt caacgttttg atataactga agatgtaatc ccaacgaccg
     5641 cagttcagac caacaatatg gtcacgcagc gcgtgaagga tttcatccat ctggaacacg
     5701 gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc cgcgcggcag attaaagcga
     5761 tcttctgcat agctgaagac ttcgctccac caggccgctt cctgccagga ctgggttttc
     5821 ggcagataga aatagggacc actgcccttt gccaacagtg cctgatagtt gtggaagaaa
     5881 tagagcgcaa aatcaaacag gctgccgggg attgcctcac cacgccaggt gacatgtttt
     5941 tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg ctggattggg cttgagctgg
     6001 taaattttgc ctgcttcatt ggtgtaactg atggtgccgt taaccgcatc acgcaggtta
     6061 atttgcccgt cgatcacttt gttccagtct ggtgccagtg aatcttcgaa atcggccata
     6121 aagactttca cattggcgtt cagtgcgttg atcaccatct tgcgctcaac cgggccagtt
     6181 atctcgactc gacgatcttg taagtccgcg ggaataccac gaattttcca gtctgtatcg
     6241 cgaatggaag ctgtttccga aataaaatca ggcaacgttc cgttatcaat atcttgctgc
     6301 tgctgaatgc gcgctgccag aagtttattg cgttgtggcg taaaatgcgt caccagctca
     6361 gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct cctgctcgcc atacggcctt
     6421 atgaaagcca gttcatcggt tgttgttgcc tgttcagtca tcgtgcagct cctcgtcatg
     6481 gatccgaaaa cttccccact gtgaacgaag gatcgttgtg cgcttttcgt tcagcacaac
     6541 taagactact catttaaaat gcaaaatcaa aaacaatttc cattttaatt a
//
LOCUS       DAZUCE010000257         6580 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZUCE010000257 DAZUCE010000000
VERSION     DAZUCE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.257"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            130..372
                     /gene="yjbE"
                     /locus_tag="ACHY03_004451"
     CDS             130..372
                     /gene="yjbE"
                     /locus_tag="ACHY03_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFS3907605.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            487..1125
                     /locus_tag="ACHY03_004452"
     CDS             487..1125
                     /locus_tag="ACHY03_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFS3907606.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQCMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1122..1859
                     /locus_tag="ACHY03_004453"
     CDS             1122..1859
                     /locus_tag="ACHY03_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFS3907607.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1859..3955
                     /locus_tag="ACHY03_004454"
     CDS             1859..3955
                     /locus_tag="ACHY03_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFS3907608.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIRTIRITENRLNLPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4002..4280)
                     /locus_tag="ACHY03_004455"
     CDS             complement(4002..4280)
                     /locus_tag="ACHY03_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907609.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4281..4424
                     /locus_tag="ACHY03_004456"
     CDS             4281..4424
                     /locus_tag="ACHY03_004456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907610.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4550..4960
                     /gene="psiE"
                     /locus_tag="ACHY03_004457"
     CDS             4550..4960
                     /gene="psiE"
                     /locus_tag="ACHY03_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFS3907611.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5004..6479)
                     /gene="xylE"
                     /locus_tag="ACHY03_004458"
     CDS             complement(5004..6479)
                     /gene="xylE"
                     /locus_tag="ACHY03_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFS3907612.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1630 a         1684 c         1768 g         1498 t
ORIGIN      
        1 attccaatta tcctaaaacc ccatctctaa ttccccgcgc cgtaattcgc atgctttagt
       61 tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat tcaatgaagg
      121 gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct tgcggcgact
      181 tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag ggtcggcagc aacgtcggtt
      241 tcggcgggta gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg
      301 ctcgcggcaa ccggtggcgg tgatggttct aataccggga ccacaacaac cacgaccacc
      361 agtacccagt aataaagtat gtatccccaa aataattcga gtcatcgcat ctgcagcttg
      421 aagtatgaag gggattaacc ataaccacac tccggtgtgg ttattctgcc cctctggaga
      481 agagtcgtga agcgacctgc actcattctt atctgcctgc tattacaggc ctgttcagcc
      541 acgactaaag agctgggcaa ttcactgtgg gacagtctgt tcggtacgcc aggtgtacag
      601 ctgacggacg atgatattca aaatatgccc tacgccagcc agtgcatgca gcttaatggc
      661 gggccgcagt tgtttgtggt gctggccttc gctgaagacg gacaacaaaa atgggtcact
      721 caggatcagg ccactctcgt cacacaacat ggtcgtctgg tgaagactct gcttggcggc
      781 gacaacttga ttgaggtgaa taaccttgcc gccgacccgc tgattaaacc cgcacaaatc
      841 attgatggtg caacctggac gcgcacgatg ggctggaccg aataccagca ggtacgctac
      901 gccaccgcac gctcagtctt caaatgggat ggcaccgata ccgtcaaagt cggcagcgat
      961 gaaaccccgg ttcgcgtgct ggacgaagaa gtctccaccg accaggcgcg ctggcataac
     1021 cgctattgga tcgacagcga agggcaaatt cgccagtcgg aacagtatct cggcgcggat
     1081 tatttcccgg tgaaaaccac cctcatcaag gcggcaaaac aatgatcaaa caaacttttg
     1141 tcgcgttgct tttgagtgtg ggagcgtcat cggtcttcgc ggcaggaacc gtcaaggtgt
     1201 tcagcaatgg cagcagtgag gccaaaacac taacaggcgc agagcattta atcgatctgg
     1261 taggccaacc gcggctggca aacagctggt ggcccggtgc ggtgatcagc gaagagctgg
     1321 caacggcggc agcattgcgt cagcagcagg cgttgctgac acggctggca gaacagggcg
     1381 cagattccag cgccgacgat gccgctgcga ttaacgcctt acgccagcaa attcaggcgt
     1441 tgaaggtgac gggcaggcaa aaaatcaatc ttgatcccga tatcgtccgc gttgctgaac
     1501 gcgctaaccc gccgttgcag ggcaactaca cgctgtgggt cggaccacca ccgtccacgg
     1561 tcacgttgtt cgggcttatc agccgtcctg gcaatcagcc attcactccc ggtcgcgacg
     1621 tggcgagcta tctctccggg caaagtctgc ttagcggtgc ggatcgcagc tacgcgtggg
     1681 tggtttaccc ggacggacgc acacaaaaag cgccggtggc ttactggaat aagcgtcacg
     1741 tagagccgat gcccggcagc attatttatg ttggcctcgc agactccgtc tggagtgaga
     1801 cgcctgatgc ccttaacgcc gacattcttc agactctgac gcagcggata cctcaataat
     1861 gaaaaaaaga catctgctta gcttactggc gctgggcatt agcacagctt gctacggcga
     1921 aacatatcct gcgcccattg gtccgtcgca gtcggatttc ggtggcgtag gattattaca
     1981 aacgcccacc gcgcgtatgg cacgggaagg ggagttgagt ctgaactatc gcgataacga
     2041 tcagtaccgt tattactcag cttcagtgca actcttcccg tggctggaaa caacgctgcg
     2101 ctacaccgac gtgcgcaccc ggcagtacag cagcgtcgaa gcgttctctg gcgatcaaac
     2161 gtataaagat aaagccttcg atctcaaact gcgtttgtgg gaagagagtt attggctgcc
     2221 gcaagtggcg gttggtgcgc gggatatcgg cggtacgggg ctgtttgatg cggaatatct
     2281 tgttgccagc aaagcctggg ggccgttcga ttttacgctc ggcctgggct ggggatattt
     2341 gggcaccagc ggtaatgtga aaaatccgct ctgttcagcc agtgataaat attgctatcg
     2401 cgataacagc tacaaacaag cgggatctat cgacggcagt cagatgttcc acggcccggc
     2461 ctcactgttt ggcggcgtgg aataccagac gccctggcaa ccgctgcgcc tgaaactgga
     2521 gtatgaaggt aataattatc agcaggattt tgccgggaag ctggagcaaa aaagtaagtt
     2581 taacgtcggg gcgatttatc gcgttaccga ttgggccgac gttaacctta gctatgaacg
     2641 tggcaacacc tttatgtttg gcgttacgtt acgcaccaac tttaacgatc tgcgcccgtc
     2701 ttacaacgat aacgcccgcc cgcaatatca accgcagccg caggatgcca ttttgcagca
     2761 ttcggtggtg gcgaatcagt taacgctgtt gaaatacaat gccggacttg ccgatccaca
     2821 gatccaggcg aaaggcgata cgctgtatgt taccggcgag caggtgaaat atcgtgattc
     2881 gcgcgaaggg atcatccgtg ctaatcggat cgtgatgaac gatctgccgg atgggatcag
     2941 aacgatccgc attacggaaa atcgtcttaa cctgccgcag gtgacgacgg aaaccgatgt
     3001 cgccagcctg aaaaatcatc tcgccggaga gccgttgggc cacgaaacga agctggcgca
     3061 aaaacgcgtc gagccagtgg ttccgcagtc caccgagcag ggctggtata tcgacaaatc
     3121 acgctttgat ttccatatcg atccggtgct gaaccagtcg gtcggtggcc cggaaaactt
     3181 ttacatgtat cagctcggcg tgatgggaac ggcagatttg tggctgacgg accatctgct
     3241 gaccaccggc agcctgtttg caaatcttgc caacaactac gacaagttta actacaccaa
     3301 tccgccgcag gactcgcact taccgcgcgt gcgtacccat gtgcgcgagt atgtgcagaa
     3361 tgatgtctat gtgaataacc tgcaagccaa ctacttccag catctgggca acggcttcta
     3421 cggtcaggtc tacggtggtt atctcgaaac catgtttggc ggtgcggggg cagaagtgtt
     3481 gtatcgcccg ctggacagca actgggcgtt tggtctggat gccaactacg ttaaacagcg
     3541 cgactggcgt agtgcaaagg atatgatgaa attcaccgac tacagcgtga aaaccggaca
     3601 tctgaccgcc tactggacgc catctttcgc tcaggatgtg ttagttaaag ccagcgtcgg
     3661 gcagtatctg gcaggggata aaggcggcac gctggagatc gctaaacgct ttgacagcgg
     3721 cgtggtggtg ggcggctatg ccacgatcac caacgtttcg aaagaggagt acggcgaagg
     3781 ggacttcacc aagggcgtgt atgtctcggt accgttggat ctcttctcgt ctggcccgac
     3841 acgcagccgt gcggcgattg gctggacgcc gctgacgcgt gacggtggtc agcaacttgg
     3901 gcgtaagttc cagttgtatg acatgaccag cgaccgtagc gtcaatttcc gctaagtcat
     3961 gggaaaggtg ccagttttcg caatcactgg caccctcatt cttaatggcc tgttgagcca
     4021 tcaggaacgc agttcacccc cggtttgcga tctggtgtgt aaaggcgaaa acattctgtc
     4081 ttcctgtccg tcgatatcgg ttgcaggtct ggttgcctga gcgactgggc catcattggg
     4141 ctggagacat tgctttgcgg taggtcggct ttatcagcag taccgagcgg accagcataa
     4201 gcaggaagaa cagagactga taacatcaaa gcagcaaaat aaggcttcat ttttaccacc
     4261 tttatcaggt tacgtttcat ttgttccaga ggaacattgt cgatttttcg cgcattgctg
     4321 gtggctggga atcacctgaa tgggtgattt ttgaattacc ggctttggtg cggtttgtct
     4381 tgccggatgc gccgccaggc gcggcttatc cggcctacgg gtaggtatat ccggctttgg
     4441 tggaggcgcg ctccaaatcc aggttgaaca aaacatacac aaaaaatata gatctccgtc
     4501 acatttttgc gttatacagg aagctcgcca ctgtgaagga ggtactgcta tgacgtcact
     4561 ctctcgtccg cgcgtggagt ttatctccac cattttgcag accgtactca atcttggcct
     4621 gttgtgcctc ggcctgattt tggttgtctt cctcggcaaa gaaacggtgc atctggctga
     4681 tgtgctgttc gcgccagaac aaaccagcaa atatgagctg gtagaaggac tggtggttta
     4741 ctttctctat ttcgaattta tcgcgctgat tgtgaagtac tttcagtccg gttttcactt
     4801 cccgctacgt tactttgtct atattgggat caccgccatt gtgcgcttga tcatcgtcga
     4861 tcataaatcg ccactggatg tgttgatcta ctcggctgcg atcctgttac tggtgatcac
     4921 cctgtggctg tgtaattcga aacggctgaa gcgggagtaa aaagacagca cgccgaactg
     4981 gcgtggcgtg ctggacagga agattacagc gtagcagttt gttgtgtttt cttcgtttcc
     5041 ggttcccaga gggcttccag ctcctcaagg gttttacctt tggtttccgg gacaaatttc
     5101 cacataaata gtgctgccag aacgcccata cacccgtaaa tccagtagga gaaaccgttg
     5161 tggaaatggg ccaccagcca ggagtttttg tccatcatcg ggaaggtcca ggagacgaag
     5221 tagttcgcca gccactgggc cgccaccgcg attgccagcg ctttaccacg aatggcattc
     5281 gggaagattt ccgacagcag aacccagcat accggacccc aggacatggc aaaggcggca
     5341 acatagaaca gcatcgacag tagcgccaca atacccggtg cctgagtgta aaacgcggta
     5401 ccgaggctaa acataccgat tgccattccg agcgcgccga taatttgcag tggcttacga
     5461 ccaaatttat ccaccgtcat aattgccaga acggtgaagg tgaggttgat aactccgaca
     5521 ataatggtct gcaaaagcgc gatatccgtg ctggccccca gcgttttgaa cacttccggc
     5581 gcgtagtaca acaccacatt gatgccgaca aattgctgga agatggagag cataacgccg
     5641 attacaatca cgcctacgcc aaacatcagc agacgaccac cggttttgcg gccatgatcc
     5701 agagagtgtt taatttcctg tactgcctga gttgcaagcg tgttgcccat aattttgcgc
     5761 aggatacctt ccgcctgttc ttgcttgccg cgtgacatca gccagcgagg actttctggc
     5821 acggtataca gcagcattaa gaacagcagt gcagggatac attccgaggc aaacatataa
     5881 cgccagccgt cagtattcag ccaggaggcg tcgccggaac gggcaataaa atagttcaca
     5941 cagtaaacta aaagttgccc gaaaataatc gcaaactggt taaatgaaac cagtttcccg
     6001 cgaatatgag caggagccag ttccgcaata tacattggtg aaagcattga ggctaaacca
     6061 acgccaatac cgccaataat acgataaata acaaattccg ggacataacc tgccagataa
     6121 acaggcactg tgttgtccgg gtttatagag gtaaaaccaa gttctggcca ggcagaacct
     6181 acaccagaaa taaaaaacag gacagcagca atcttaagtg aatcacgacg accgaagcgg
     6241 ttactgcaat aaccaccgag ggcaccgccg atgatgcaac caatcagagc gctggccacg
     6301 caaaacccta acagggagtt ggcagcggat tcacttaagt tttgtggagc aacaaagacg
     6361 gtattgagtg actcaacagt accggaaata acggcggtgt cgtagccaaa taataaacca
     6421 cctaatgtag cgactaaggt aatcgaaaat atataactgg aattatactg ggtattcatt
     6481 cagacctgcc ttagaccatt ctgatctttt atcagtgcat tgatgttgga attgtcaggc
     6541 agttaaggca attatcattt tttacaatgc gtttacgtaa
//
LOCUS       DAZUCE010000258         6551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZUCE010000258 DAZUCE010000000
VERSION     DAZUCE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.258"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            386..2536
                     /gene="dinG"
                     /locus_tag="ACHY03_004459"
     CDS             386..2536
                     /gene="dinG"
                     /locus_tag="ACHY03_004459"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFS3907613.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLTQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2564..3526
                     /gene="ybiB"
                     /locus_tag="ACHY03_004460"
     CDS             2564..3526
                     /gene="ybiB"
                     /locus_tag="ACHY03_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFS3907614.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3667..4752
                     /gene="hcxB"
                     /locus_tag="ACHY03_004461"
     CDS             3667..4752
                     /gene="hcxB"
                     /locus_tag="ACHY03_004461"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFS3907615.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDGGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            4819..4878
                     /gene="ybiE"
                     /locus_tag="ACHY03_004462"
     CDS             4819..4878
                     /gene="ybiE"
                     /locus_tag="ACHY03_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFS3907616.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(4981..5241)
                     /gene="ybiJ"
                     /locus_tag="ACHY03_004463"
     CDS             complement(4981..5241)
                     /gene="ybiJ"
                     /locus_tag="ACHY03_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFS3907617.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5507..5773)
                     /gene="ybiI"
                     /locus_tag="ACHY03_004464"
     CDS             complement(5507..5773)
                     /gene="ybiI"
                     /locus_tag="ACHY03_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFS3907618.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(5847..6524)
                     /gene="ybiX"
                     /locus_tag="ACHY03_004465"
     CDS             complement(5847..6524)
                     /gene="ybiX"
                     /locus_tag="ACHY03_004465"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFS3907619.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
BASE COUNT         1549 a         1626 c         1826 g         1550 t
ORIGIN      
        1 aaccagttgc gtataagttc gttatatcgt gatatggtcc gctatgcaca atatcgtttt
       61 tctaaagttt tatccgagtg gacgccagac gcatgggtgt aaagattgca gctgaataat
      121 ggcattgatg gtcctggctc aggccatggc gggcaatacc ggttcctgcg aagcgatgac
      181 ggcagcaagt gtaaccacga aatgcggtat cacttgctgt tttctgccct gaagactggc
      241 gctcactgtg ccgtcgaact ttcaatcaaa tatattgcga gagggcgtgt ttgcgagccg
      301 ctttccagaa acagaaaaac cattacccct gaaaaccgaa aaatgccaca atattggctg
      361 tttatacagt atttcaggtt ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc
      421 cgcctggtat aaggcgcttc aggaacagat ccccgacttt attccccgtg cgccgcagcg
      481 gcagatgatt gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc atctggcgat
      541 tgaagccccc accggcgttg ggaaaacgct ctcttatttg atccccggca tcgccattgc
      601 ccgcgaagag caaaaaacgc tggtggtgag taccgccaac gtggcgttgc aggatcagat
      661 ctacagtaaa gatttaccgc tgctgaaaaa gatcattcca gatcttaaat tcactgccgc
      721 ttttgggcgt gggcgctacg tttgcccgcg taatctgacg gcgcttgcca gcactgagcc
      781 cacgcaacag gatctgctgg cgtttcttga cgacgagctg acgccgaaca atcaggaaga
      841 gcaaaaacgt tgtgcgaagc tgaagggcga tctcgacact tataaatggg atggtctgcg
      901 cgatcatacg gatatcgcta tagatgacga tctctggcgt cgtttaagta ccgacaaagc
      961 cagctgcctc aaccgcaact gttactacta tcgcgaatgc ccgttttttg ttgctcgtcg
     1021 ggagattcag gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga
     1081 aagcgaagcg gtattgcctg acccgaaaaa tttactgctg gtgctggacg aaggtcatca
     1141 cctgccagat gtggcgcggg atgcgctgga gatgagtgcc gaaatcaccg cgccatggta
     1201 tcggctacag ctggacttgt tcacgaaact ggtcgctacc tgcatggagc agtttcgccc
     1261 gaagaccatc ccgccgttgg cgatccctga acgtttgaat gcgcattgtg aagagttgta
     1321 tgagcttatc gcctcgttaa acaacattct caatctctac atgcctgccg ggcaggaggc
     1381 agagcaccgt tttgcaatgg gtgaactgcc tgatgaagtg ctggagatct gccagcggct
     1441 ggcaaaactc accgagatgc tgcgtggtct ggcggagtta tttcttaacg atttaagtga
     1501 gaaaaccggc agccatgaca ttgtacgtct gcatcggttg attttgcaga tgaaccgcgc
     1561 gttggggatg ttcgaggcgc aaagcaaact ctggcggctg gcttcgctgg cgcaatcttc
     1621 cggtgcgcct gtcactaaat gggcgacgcg agaagagcgc gaagggcagc tgcatctctg
     1681 gtttcactgc gtgggaatac gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat
     1741 tccgcacatt attgtcacct ccgcaacctt gcgttcgctg aacagttttt cgcgtttgca
     1801 ggagatgagc gggctgaaag agaaagcggg cgaccgtttt gtggcgctgg attccccctt
     1861 taaccactgc gaacagggca aaattgttat tccccggatg cgcgttgagc cttccatcga
     1921 caacgaagaa cagcatattg ctgaaatggc ggcctttttc cgtgagcagg tggagagcaa
     1981 aaaacatctc ggtatgttgg tgctgtttgc cagcgggcgt gcaatgcagc gctttctcga
     2041 ctatgtgacg gatttacgtc tgatgttgct ggtgcagggc gatcagccgc gttaccgttt
     2101 agttgaactg caccgcaaac gcgtcgccaa cggtgagcgt agtgtgctgg tgggcttaca
     2161 gtcatttgcc gaagggcttg atttgaaagg tgatctgctc acccaggtgc atatccacaa
     2221 aatcgctttt ccgcccatcg acagcccggt ggtgatcacc gaaggggaat ggctgaaaag
     2281 cctcaaccgc tatccgtttg aggtgcaaag cctgccgagc gcctcgttta acctgattca
     2341 gcaggttggg cgactgattc gaagccacgg ttgctggggc gaagtggtga tttacgataa
     2401 acgcttgctg accaaaaatt atggcaagcg actactggat gcattaccgg tatttccgat
     2461 agagcaaccg gaagtccctg aaggtatagt taaaaagaaa gaaaaaacga aatccccacg
     2521 ccgtcggcgg cgttaatgat gtgagtcagg taaggagtcg taaatggact atcgcaaaat
     2581 cattaaagag atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg
     2641 cggtctgtat gcccatatgc tcaatggtga agtccctgac cttgagttgg gcggcgtatt
     2701 gattgcgctg cgtatcaaag gggaagggga agcagagatg ctcggctttt acgaagccat
     2761 gcaaaatcac accatcaagc tgacgccgcc tgcaggcaag ccgatgccga ttgtcatccc
     2821 cagttacaac ggcgcgcgta aacaggccaa cctgacgccg ttgttggcga ttctcctgca
     2881 caaactcggt tttcctgtgg tggttcacgg ggttagcgaa gatccaactc gcgtgctgac
     2941 agaaaccatt ttcgaactga tgggtattac gccaacgttg cacggcgggc aggcgcaggc
     3001 gaagctcgac gaacatcaac cggtgtttat gcctgtcggc gcgttttgcc cgccgctgga
     3061 aaaacaactg gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa
     3121 actggcgacg ccatttgccg aaggtgaggc actgcgtctt tccagcgttt cgcatccgga
     3181 atacattgga cgcgtcgcaa agttctttag cgatatcggc gggcgggcgt tactaatgca
     3241 tggtactgaa ggcgaagtgt atgctaatcc gcagcgctgc ccgcagatca atctcattga
     3301 ccgtgaaggg atgcgggtgc tgtatgaaaa acaggacact gctggtagcg agttactgcc
     3361 acaagcaaaa gatccggaaa ccacggcgca gtggattgag cgttgcctgg ctggcagcga
     3421 accgattccc gaatcgctga aaatccagat ggcttgctgc ctggtggcta cgggtgaagc
     3481 ggcaactatc agcgatggcc tggcgcgcgt taatcaggca ttttaatttt tatctcagcc
     3541 ggatgagcca cgtcatccgg cttttacccc accgcaatat gaaattcctg catctttatt
     3601 gaccttctcg cgcccggcgt gcagcataaa aaaacaacaa acacataaca taaacaggag
     3661 ttaaccatgg aaagtggtca tcgctttgat gctcagacgc tgcacagctt tattcaggct
     3721 gtatttcgtc agatgggtag cgaggaacaa gaagcgaaat tagttgccga tcatttaatc
     3781 gcggcaaacc tggcagggca tgattcacat ggtattggca tgatcccaag ctatgtacgc
     3841 tcctggagtc aggggcacct gcaaattaac catcatgcca aaatcgttaa agaggcgggg
     3901 gcggcggtca cgctcgatgg cgatcgcgca tttggtcagg tcgcggcaca cgaagcgatg
     3961 gcgctgggga ttgagaaagc gcatcagcac ggtattgctg ccgtggcgct acataactcg
     4021 catcatatcg gccgtatcgg ttactgggca gaacagtgcg cagcggcggg gtttgtctct
     4081 atccactttg ttagcgtggt cggtattcca atggtcgcgc cgttccacgg tcgcgacagc
     4141 cgctttggca ccaatccgtt ctgtgtggtt ttcccacgta aagatgattt tccgctgttg
     4201 cttgattacg ccaccagcgc cattgcattt ggcaaaaccc gcgtcgcctg gcataaaggc
     4261 gtccccgtgc cgccaggttg cctgattgac gttaacggcg tgccgacgac caatccggcg
     4321 gtaatgcagg agtcgccgtt ggggtcgctg ttgacctttg ccgaacataa aggctacgcc
     4381 cttgccgcga tgtgtgaaat tcttggcggg gcgctttctg gcggtaaaac gacgcatcag
     4441 gaaacgttac aaaccagtcc cgatgccatt cttaactgca tgaccactat catcatcaac
     4501 ccggaactgt tcggcgcgcc ggattgtagc gcgcagactg aagcctttgc cgagtgggtg
     4561 aaagcctcgc cgcatgatga cgataagccg attttgctac cgggcgagtg ggaagtgaac
     4621 acgcgtcgcg aacggcagaa gcaggggatt ccattggatg ggggaagctg gcaggccatt
     4681 tgtgatgcgg cgcggcagat tggtatgtcg gaagagacgt tgcaggcttt ctgtcagcag
     4741 ttagccagct aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt aacataggat
     4801 caatgagggt tagagcatat gcgtctgtcg gcaaacagac agggaaatac ttgtgctgga
     4861 cgtagcgtaa acgcctgatc cgtccaacgg ttcggatttt gtaggcctga taagacgcgg
     4921 tagcgtcgca tcaggcatct gttgccggat gcggcgtaaa cgccttatcc gtcctacgaa
     4981 ttacttataa attaccgcag taccgcttaa tttattgttg ttggtggcgg aagtgatgct
     5041 gtatccactc gccccagctg ccgccgcttt ctcagccagt ttcgcttcca gggcatcgag
     5101 ggtggatgcg ccatcggcag aaaccacgcc gattttattc atgttctgtg cctgggatgc
     5161 cgttaccggt tccgcggcaa acacgccaaa tgacagagtt gaaagagcca tagcagcaac
     5221 aacagtattg atagttttca taattaatct ctcgcaggtg attttttgtt taagtgacgt
     5281 tgtttcgtcg atgtgatgag tatcacgttt ttttacgaga gataaaatcg aagagaattg
     5341 accgccttgt tcaaataaat tgattgatat ctaacatact gataaaccat ttatttgttg
     5401 tacttatgga cccgttttat atcgcgtggc agtcacggca gcgttaaagg cacggaatgc
     5461 cacgaaaaat acaaattata ctcgctatac atcccgccag ttgtagttaa cgtaactggc
     5521 tgtctttcga acctctgcga ttatatcctg tataagctgg tttttgtaaa tctttctcct
     5581 gctgacaatg aatacataag cgcacgccag gaatggcttc ccgacgggct tgcgggatgg
     5641 gggcaccgca ctcttcacat tcatacaggc tttcgccacg cggaatttca ccccgagcgc
     5701 gggcaatcgc atcttcaatt gtactgttga tctgttcgtt gacggcgtca tcgttagccc
     5761 aaccggatgc catatcgtcc tccccatatc aatgcttgta cagttaagtg tagctaatat
     5821 agggacgaac tcgggtagtt caagcgtcag atctccgacc attcccgcag cagattatga
     5881 taaagattga gcagcgacag gatctcttca ctttcgccgt agcggctttt cagcgactga
     5941 atattgttgt ccagttcaaa cagcatggcg cgctttttat catcgcggat catcgactgg
     6001 atccacataa atgatgccac tcgtacgccg cgggttacgg gtgtcacgca atgcaggctg
     6061 ctggagggat acaacaccag atcacctgcc gggagtttta cccgatgttg tccgaaggtg
     6121 tcattaacga ccagttcgcc gccgtcgtag ctttgtggat cgcttaaaaa cagcgtggca
     6181 gaaaggtcag tgcgcatcca gccgttttgt ggatggctgc gtactgcgcc atccacatga
     6241 aaaccatagg tttcattgtt ctgatagcga ttaaacagcg gcgtggaaag ggtacgcggc
     6301 aaggccgcgg caaagaacaa agcatgttgg ttaaccgcgt tcagcacctc attttgcagg
     6361 gcggcgtata acgtgctgcg ggtgtctacc tgttgattgt tcttaacttg cgcaccttgt
     6421 gcgccggtgg tgacgcgtcc atccacccat tcggcttgtt ccagttgttc gcgaaaacgg
     6481 gcgacgtcct gtggcgataa cacgccggga atgtggtaca tcatacaagt tctccaaaaa
     6541 gtggggcctg c
//
LOCUS       DAZUCE010000259         6500 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZUCE010000259 DAZUCE010000000
VERSION     DAZUCE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.259"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(64..2097)
                     /gene="betT"
                     /locus_tag="ACHY03_004466"
     CDS             complement(64..2097)
                     /gene="betT"
                     /locus_tag="ACHY03_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFS3907620.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2226..2813
                     /gene="betI"
                     /locus_tag="ACHY03_004467"
     CDS             2226..2813
                     /gene="betI"
                     /locus_tag="ACHY03_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFS3907621.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2827..4299
                     /gene="betB"
                     /locus_tag="ACHY03_004468"
     CDS             2827..4299
                     /gene="betB"
                     /locus_tag="ACHY03_004468"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFS3907622.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPSLEGRQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTILREEIFGPV
                     MSILTYESEEEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4313..6001
                     /gene="betA"
                     /locus_tag="ACHY03_004469"
     CDS             4313..6001
                     /gene="betA"
                     /locus_tag="ACHY03_004469"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFS3907623.1"
                     /translation="MQFDYIIIGAGSAGNVLASRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNHRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLEHWSYLDCLPYYRKAETRDIGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSTE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQSDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQGALPRSTAGYFVANGMPVRAKKMSRDLN"
BASE COUNT         1597 a         1785 c         1769 g         1349 t
ORIGIN      
        1 ttacagcgag tcaatgattc ccgcgccata tgcagacatg gcgcggtttt atgcaaaaac
       61 aatctacgcg tccgggaaca tcacgctatg gcccggcgct tcacgatgga gatgaataaa
      121 gttaaggtgc cgctcgtact ggtcaagaat atcggtgatc acctgctctt tgctgtagtc
      181 catcaggtcg ttgccctggc tgccttctaa caggaaggtt tccagccggt agtaggtcga
      241 tttcccgcta cgtgcgcggt aggtaaagcc cggcaccgag tattgctgcg gccaaatctg
      301 atagacaaag ttttgctctt cgcccatatg caccaacaaa tccagatgcc ccaactgttg
      361 tccctcttcc ggcggtaggc tttttagctc cacatacgcg ccgcgcaacc gcagctcttg
      421 cgccacttct tccattgccg ggtaacagac cgtctccatc atctgtttag tgtaacgcgt
      481 gcccggataa ttcatcaggc gcgagagacg ttttttccaa ctcaggcgat cctgaagccc
      541 cagcggtcgc ggcgcggtat cgcggttggc actttcacgg cggtaatctt ctactttcag
      601 agatttatac aaccccgcca tcacgaagaa gatcacaaag ctgaacggca gccccataat
      661 caccgtggtg ttttgcagcg cggatatccc gttggtcatc agcatgccga gcgtcagcag
      721 gccaatcgcc accgaccaga agacgcgcag ccagccgggg gcgtcgctgt tgatatcttt
      781 aagctgcgag gtgaaattcc ccagcaccag cgccccggag tccgctgagg tcacataaaa
      841 cagcaggcca gtaatggtgg cgacggaggc gctaaaggta aacgccggat actgcgccag
      901 caggctgtag aagccgcgct ccggatggac catcgcttcc tcggcaaatg ccgcgccgcc
      961 gtggatgatt tcatacagcg cgctattgcc gaacaccgag agccataaca gcgtgaaggt
     1021 aaacggaata atcaacgtgc ccagcacgaa ctggcgaatg gtacgcccac gcgagatacg
     1081 cgccaggaac aagccgacaa acggcgacca tgccacccac catgcccaga agaagagcgt
     1141 ccagttattc atccactcaa ctggacggtc gaaggcaaaa ctgttgagcg tcatgcccat
     1201 aaagcgattc acatagtcgc caacattcag caccagtgca ttaagcagga acgaagtgtc
     1261 gcccataaac aacacgaaca ggatcaatcc cagcgccagc gcgacattaa gctccgataa
     1321 cacgcgaatg cccttatcga caccggatgt caccgagata gtggcgatta tcaccgacaa
     1381 ggcgatcagt gccgcttttg ccgccatcga atcgggaata tcaaacagta cgctcaagcc
     1441 atagttaagc tgcaccacac cgataccgag cgtagtggca ataccgaaga tagtgccgat
     1501 caccgctgca atatccactg agtgacctat cggcccgtta atccgtttac cgaagatcgg
     1561 gtacagcgcc gaacggatgg tgagcggcaa attataacga tagctaaagt atccgagcgc
     1621 catgcccatc agcgcataca tcgaccagcc ggttaagccg tagtgaaaca gcgtccagac
     1681 catcgcctga cgcgcggcct caatcgtctg tcccgcgcct tccggcggct gcatatactg
     1741 cgttaccggt tcggctacgg aaaagaacat cagatcgata ccgatcccgg cagcaaacag
     1801 catcgccgcc caactcagca ggctgaattc cggtttggat tgttctggcc cgagcttcac
     1861 cgaaccaaaa cgcgaacaag cgatacagac cacaaagaca atatagagcg ttgccgccag
     1921 cagatagtac caaccgaagg ttttagaaac ccagtccagc gtgcggccaa tccacagggc
     1981 cgagaagtcg cgaaacagga tcgttgtcag ggaaaacaac aaaatcagtc cggcggaggt
     2041 gtaaaacacc accggattga ttttgtcctt ttccctgctg tgtgaaaggt ctgtcatcca
     2101 gtatccccac tgttattgtt accatttaaa atcaaattcg taacaattaa gacacatttt
     2161 atattgaacg tccaatcaat aaccgcttta atagataaac aacgctgatg aatggagtgg
     2221 cgaaaatgcc caaattgggg atgcagtcga tccggcgcag acaactgatc gacgccacac
     2281 tggaagcaat aaatgaagtg ggcatgcacg atgcaacgat cgcgcagatc gcccgccgtg
     2341 caggcgtttc tacggggatc atcagccact atttcaggga caaaaatggt ctgctggaag
     2401 caaccatgcg cgatatcacc agtcagctac gtgacgcggt tttgaatcga ttacatgcac
     2461 ttccgcaggg cagtgcagaa caacgattac aggcgattgt tggcggaaac ttcgatgaaa
     2521 cgcaggtgag cagtgcggcg atgaaagcct ggctggcgtt ctgggccagc agtatgcatc
     2581 agccgatgct ctatcgttta cagcaggtca gcagccgccg cttgctgtcg aatctggtga
     2641 gcgagtttcg ccgcgaattg ccgcgccacc aggcacagga agcgggctac ggccttgccg
     2701 cgctgattga cggattatgg ctgcgcgcgg cactgagcgg caaaccgctg gataaacctc
     2761 tcgcacactc gctgacccgt cactttatta ctcagcattt acccaccgat taaccgagga
     2821 gacgtgatgt cccgaatggc agaacagcag ctttatatac atggtggtta tacctccgcc
     2881 accagcggtc gcactttcga gaccattaac ccggccaacg gtaacgtgct ggcgaccgtg
     2941 caggccgccg ggcgtgagga tgtcgatcgc gccgtgaaga gtgcccaaca ggggcaaaaa
     3001 atctgggcgg cgatgaccgc tatggaacgc tcgcgtattc tgcgtcgggc cgtcgatatt
     3061 ctgcgcgagc gcaatgatga actcgccaaa ctggaaaccc tcgacaccgg aaaagcgtat
     3121 tcggaaacct caaccgtcga tatcgttacc ggtgcggacg tgctggagta ctacgccggg
     3181 ctgatcccgt cgctggaagg ccgccagatc ccgttgcgtg agacatcatt tgtctacact
     3241 cgccgcgaac cgctgggcgt ggtggcaggg attggcgcat ggaactaccc gatccagatt
     3301 gccctgtgga aatccgcccc cgcgctggcg gcgggtaatg cgatgatttt caaaccgagc
     3361 gaagtcactc cgcttaccgc gttaaagctg gctgaaattt acagcgaagc gggcctgccg
     3421 gacggtgtat ttaacgtgtt gccgggcgtg ggcgcggaga ccgggcaata tctgaccgag
     3481 catccgggca ttgccaaagt gtcgtttacc ggcggcgtcg ccagcggcaa aaaagtgatg
     3541 gctaactcgg cagcctcttc cctgaaagaa gtgaccatgg aactgggcgg taaatcaccg
     3601 ctgatcgttt tcgacgatgc ggatctcgat ctcgccgccg atatcgccat gatggcgaat
     3661 ttcttcagct ccggccaggt gtgtaccaac ggcacccgcg tcttcgttcc ggcgaaatgc
     3721 aaagccgcgt ttgagcaaaa gattctggcg cgcgttgagc gtattcgcgc gggcgacgtt
     3781 ttcgaccctc aaaccaattt tggcccgctg gtcagcttcc cgcatcgcga taacgtgctg
     3841 cgctatatcg ccaaaggcaa agaggaaggc gcgcgcgtac tgtgcggcgg cgatgtactg
     3901 aaaggcgatg gcctcgataa cggcgcatgg gtcgcgccga ctgtgttcac cgattgcagc
     3961 gacgagatga ccatcctgcg tgaagagatc ttcgggccgg tgatgtccat tctgacctac
     4021 gagtcggaag aggaagtcat tcgccgcgct aacgataccg actacggcct ggcggcggga
     4081 atcgtgacgg cggacctgaa ccgcgcgcat cgcgtcattc atcagctgga agcgggtatt
     4141 tgctggatca acacctgggg cgaatccccg gcagagatgc ccgttggcgg ctacaaacac
     4201 tccggcattg gtcgcgagaa cggcgtgatg acgctccaga gttacaccca ggtgaagtcc
     4261 atccaggttg agatggctaa attccagtcc atattctaac caggaggttt atttgcaatt
     4321 tgactacatc attattggtg ccggctcagc cggcaacgtt ctcgctagcc gtctgactga
     4381 agatccgaat acctccgtgc tgctgcttga agcgggcggc ccggactatc gctttgactt
     4441 ccgcacccag atgcccgctg ccctggcatt cccgctacag ggcaaacgct acaactgggc
     4501 ctatgagacg gaacccgaac catttatgaa ccaccgacgc atggagtgcg gacgcggtaa
     4561 aggcctgggt ggatcgtcgc tgatcaacgg catgtgctac atccgtggca acgcgctgga
     4621 tctcgataac tgggcgaaag aacccggcct ggaacactgg agctacctcg attgcctgcc
     4681 ctactatcgc aaggccgaga cgcgcgatat tggtgaaaac gactaccacg gcggcgacgg
     4741 cccggtgagc gtcaccacct ccaaacccgg cgtcaatccg ctgtttgaag cgatgattga
     4801 agcgggcgtg caggcgggct acccgcgcac ggacgatctc aacggttatc agcaggaagg
     4861 ttttggcccg atggatcgca ccgtcacgcc gcagggccgt cgcgccagca ccgcgcgtgg
     4921 ctatctcgat caggccaaat cgcgtcctaa cctgaccatt cgtactcacg ctatgaccga
     4981 tcacatcatt tttgacggca aacgcgcggt gggcgtcgag tggctggaag gcgatagcac
     5041 cattccaacc cgcgcgacag cgaataaaga ggtgctgtta tgtgcaggtg cgatcgcctc
     5101 tccgcagatc ctgcaacgtt ccggcgtggg tagcactgaa ctgctggcgg agtttgatat
     5161 tccgctggtg catgatttac ccggcgtcgg cgaaaatctt caggatcacc tggagatgta
     5221 tctgcaatat gagtgcaaag aaccggtttc cctctaccct gccctgcagt ggtggaatca
     5281 gccgaaaatc ggtgcggagt ggctgtttgg cggcaccggc gttggtgcca gcaaccactt
     5341 tgaagcagga ggatttattc gcagccgaga ggaatttgcg tggccgaata ttcagtacca
     5401 tttcctgcca gtagcgatta actataacgg ctcgaatgca gtgaaagagc acggcttcca
     5461 gtgccacgtc ggatcgatgc gctcgccaag ccgtgggcat gtgcggatta aatcccgcga
     5521 cccgcaccag catccggcga ttctgtttaa ctacatgtcg cacgagcagg actggcagga
     5581 attccgcgac gcaattcgca tcacccgcga gatcatgcat caaccggcgc tggatcaata
     5641 ccgtggccgc gaaatcagtc ctggtgtcga gtgccagtcg gatgagcagc tcgacgagtt
     5701 cgtgcgtaat cacgccgaaa ccgccttcca tccgtgcggt acctgcaaaa tgggctacga
     5761 cgagatgtcc gtggttgacg gcgaaggccg cgtgcatggg ctggaaggcc tgcgcgtggt
     5821 ggatgcgtca attatgccgc agattatcac cgggaatttg aacgccacga caattatgat
     5881 tggcgagaaa atagcggaca tgattcgtgg acagggagcg ttgccgagga gcacggcggg
     5941 atattttgtg gcaaatggga tgccggtgag agcgaaaaaa atgagtcgtg atttgaactg
     6001 actcaagaac cgatcagatt gagagttacc gttccaggta tgggaacaga atcgttatat
     6061 aaaacatagg ggtaactctc atgcttcata gtgacaacct cgccagcaaa ttctaagcgg
     6121 actcgttcaa tctcgctgat gaaggtgcta tgaatgcact gataaaccgc aggtgaaatg
     6181 ccactcagct taagaattac gccactagtg ttcctggcta tggtgacttg ttcactcccg
     6241 ctcacggtta gcatatacga aaagggttct tgtaacgagg acaaaaataa gtcagaccaa
     6301 aacaacgtgg agctttaaac acaattcgta taatttatta gcagtgattt atttttactc
     6361 ttctttaatt aatcatcatg cttaaatatt ttttagcaca agattatcgc ttcgctaaca
     6421 ttttagttta cattgatttc caattgataa taaaaagttg ttaataagtt tatgcgaaaa
     6481 ataaaaattg ttgaaatact
//
LOCUS       DAZUCE010000260         6439 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZUCE010000260 DAZUCE010000000
VERSION     DAZUCE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.260"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(132..980)
                     /locus_tag="ACHY03_004470"
     CDS             complement(132..980)
                     /locus_tag="ACHY03_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS3907624.1"
                     /translation="MSDITASRPEVVNGYTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVMTESIGGKTARDWAMKQDIRCGCWLTEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYSSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGLHLITGQQADRLADLERMLRLFSGKPIPDSR
                     ENIIIRLDDHIRSVQGKACYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKYYPE
                     IIPYNI"
     gene            complement(1065..1262)
                     /locus_tag="ACHY03_004471"
     CDS             complement(1065..1262)
                     /locus_tag="ACHY03_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFS3907625.1"
                     /translation="MKSLTTESALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQLQLPDQNR"
     gene            complement(1274..1762)
                     /locus_tag="ACHY03_004472"
     CDS             complement(1274..1762)
                     /locus_tag="ACHY03_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS3907626.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVELAELINVVCDNGYS
                     LRVVDESDRTSTDSIPPSAALSGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDACAECLPGLPTF
                     NW"
     gene            complement(1759..2136)
                     /locus_tag="ACHY03_004473"
     CDS             complement(1759..2136)
                     /locus_tag="ACHY03_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFS3907627.1"
                     /translation="MKTLPVLPGLAASSHPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAEAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKR"
     gene            complement(2225..2593)
                     /locus_tag="ACHY03_004474"
     CDS             complement(2225..2593)
                     /locus_tag="ACHY03_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFS3907628.1"
                     /translation="MSDILPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
     gene            complement(2672..2893)
                     /locus_tag="ACHY03_004475"
     CDS             complement(2672..2893)
                     /locus_tag="ACHY03_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS3907629.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(2962..3438)
                     /locus_tag="ACHY03_004476"
     CDS             complement(2962..3438)
                     /locus_tag="ACHY03_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFS3907630.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNKLIAGEILFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSRVFSFAEHGLL"
     gene            complement(3454..3927)
                     /locus_tag="ACHY03_004477"
     CDS             complement(3454..3927)
                     /locus_tag="ACHY03_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS3907631.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDNDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(4093..4266)
                     /locus_tag="ACHY03_004478"
     CDS             complement(4093..4266)
                     /locus_tag="ACHY03_004478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907632.1"
                     /translation="MQPVPEGVIQVPESPDAPEALPGCRSISDDDLCTWCTRLLYRSG
                     ELSLCRLSTGNGI"
     gene            complement(4266..5084)
                     /locus_tag="ACHY03_004479"
     CDS             complement(4266..5084)
                     /locus_tag="ACHY03_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS3907633.1"
                     /translation="MRLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(5305..5715)
                     /locus_tag="ACHY03_004480"
     CDS             complement(5305..5715)
                     /locus_tag="ACHY03_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907634.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVALDKLKI"
     gene            complement(5731..6408)
                     /locus_tag="ACHY03_004481"
     CDS             complement(5731..6408)
                     /locus_tag="ACHY03_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907635.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGWK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNAEAATVKQALSCNPEAWT
                     EQPGACPTTYELYEGDAAYKAALDKVLKPVGLSGMFGKGGYMDGPGGSVTPVNINGTT
                     WVEGGGCKAHACGWDFIVTLYNPKTHEVVGYRYYGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
BASE COUNT         1508 a         1631 c         1681 g         1619 t
ORIGIN      
        1 ccggataatg ttattcatat aaaatttggg aacagatgat aaaaatacat gcatggtctg
       61 agataccctg tctactcgac catgcagtat ggttcaacta aatcagtcac aacaagaaat
      121 gattagtttt attagatatt gtatgggatt atttctggat aatacttcgc aatgatgtca
      181 tttagccgat caaccagttc tggttttctg aacgtgatat gcgcggagcc cttcttaaag
      241 tatctgatgc tgaacatctc atcttcatag caggctttgc cctgaacgga gcggatgtga
      301 tcatccagcc gaataataat attttcccgg ctgtcgggga tcggtttgcc gctgaacagg
      361 cgcagcattc tttccaggtc ggcaagtcga tctgcctgtt gcccggtgat aagatgcaat
      421 ccccaccggt cccacctcac cagattgttg acgataattt tactgccaaa tttacagggg
      481 caattggttt tgtaattcca gcttagccct ctgaagacgt tgatcacccc tcgctcaaac
      541 acttcatctt tgttctggtg cagttgttca aatgtgctca gaatgttcgc ttcactgatt
      601 tccggaaaat tatcgtactc cagtgagctg taccatgtat cacgggcctg cgcatccatt
      661 aatgacagca tcccggaacg ttgcatcagg tcacgccaga tttcgcggtc aagattgcgg
      721 gtgatggctt tcattgcggt ttccggtttc tccgtcagcc agcaaccgca acgaatatcc
      781 tgtttcatgg cccagtcccg agcggttttg ccgccaatgc tttccgtcat aactgagatt
      841 tcagccagtt gccggataag tgtgtcgatt tgcagcagtg tactctttcg tacagccaga
      901 atgtggcgga tactggttga gcagataacg tccgtatacc cgttgaccac ttccggacgg
      961 gaggctgtga tatcagacat aatcgatatt cctttttaca gataaatgaa aacgccagcc
     1021 ctgctcaggc tggcgtcagt ggaatgaaga gtggtgaatg agactcaccg gttctggtct
     1081 ggaagctgca gctggcgcag ggtatggata tcctccctgg cctgttcaat acagggcaac
     1141 agtgctgccg aatccgtttt atcttcatcg ttgtcaaaga taattcccga ttcgcagtcg
     1201 atattgtcct gcagccacgc aatcagaata tccagtgcgc tttccgtggt taatgatttc
     1261 atgttgctcc tggttaccag ttgaaagtgg gtaaacccgg caggcattca gcgcaggcat
     1321 ccagatgaat gagactgacg ccataacgtc gggtaagtgt gacaaccaga cggcggaacg
     1381 ttctggacag gcccaggcgt ttaagccgca gaaccgggta tgaccacgca tcggtacgta
     1441 acagatatcc gctgcctgta aaatgaatcc attctgattc gccggtgtca ttcgtctgat
     1501 gcgacagcga gtacagccag gcgttgtctt ttgccgtgat atgcgcggta ctgcagcgta
     1561 taccgctcag ggcagcagag gggggtatgc tgtctgttga ggtccggtca gattcatcaa
     1621 caacacgaag ggagtagcca ttatcgcaga ccacgttaat cagttcggca agctcaacac
     1681 catcgacgtc aacgacaatt cgccccatgt tcagtgcctg tacgttaacg ctgtcagctt
     1741 ccagcgtcag ggccagtttc atcgttttgc ctccggatgc ttgccctggg taatgttatt
     1801 taccattctg taattgttcc gggtcatcag gccggtcgcc ctgcgagccc ggagaatatc
     1861 aatgctgttg attaactgcg acggggcttc tgcgctgaat cccggctggt cggtacgtac
     1921 cagcgcgtat ttttcaacga gaaagttcac cgcgtcacac agtgaaatcc ctgcctcaat
     1981 atgctgctca atcacacgtt catcagcgaa cggtgtgtca ttcagcgtga ggccatagtg
     2041 ctggtccagc agtcgggaca gcagtatctg ccagatttca acaggagacg ggtgagaact
     2101 ggccgccagc ccgggtaata caggtaatgt tttcatactg aagattttcc tgatatgcag
     2161 atataaaaat ggaaaagtgg cgtggtgaaa acaccaggcc ggagcagaag gttattccgg
     2221 tggtttactt tttcgtttcg ggcgtcggat aaacagccag ataaacgtaa ccacaggagc
     2281 cgagggtgtc ggcttcgcag gtcagccctt tcgcatacag tgtgacggta tgctgatggc
     2341 ggggattcag ttcaccgccg gtgagcatga gttccagttg tttcatcagc agcggaaagg
     2401 cctggtccag gtggtacgca tctacgtcgc tgaacctgcc tctgataccg gcacggtcgg
     2461 caaggtaatg caaccggtta ccctcctgca ccagacgtgc cccgaaacag ggcgtcacgg
     2521 tgcagggcag cccccaccag gggcggtcgt gattgtcgtc gggatgcgtt gtcccgggca
     2581 gtatgtctga cacgataaag tcctcacaga aatcagttaa gaatggtccg gtggtggcga
     2641 taattctgct catcagaatg cccttcctga ttcagttcag ggtgacgctc atcagccgga
     2701 catacgggcc aaaactgtct ttacggcgtt ctgcaaacac agccagcaca ccgggaatat
     2761 cctgcacttc acgaccggta tacgcttcag tgctgccgtg ccagcggtat ttgccggtac
     2821 agaacggaaa aagacgggag gccggatgct gttggtgaat acgcatggct tcaccacggg
     2881 tgatgatttt catagtgtga tacctctgaa gacagaagat aaaagcgaaa acaggtatga
     2941 tgtggttgtg acggtgacag gttaaagcag accgtgttcc gcaaaggaga aaacccggct
     3001 gccaccgact atcagatggt ccggcacccg gatatccacc aggcccagtg cctgtaccag
     3061 acgttccgtg ataaggcggt ctgccttgct gggtgtgact tcaccggacg ggtgattgtg
     3121 cgccagcacc acggcagcag cattgtggta cagggcgcgt ttaatcactt cccggggatg
     3181 aacttccgtg cggttgatgg tgccggtaaa gaggatttca ccggcaatca gcttattctg
     3241 gttgttcaga tacagcaccc ggaattcttc acgctccagt cctgccatgt tcagaatcag
     3301 ccattcccgt gccgcacggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc
     3361 cagggttttc agggcccgca gaatgaggct gcgctcgccg ggcgtcatct ctccgggcag
     3421 aaaggaaagt tgctgcattg tccctgcctc cattcagtcg atgatgcgca taatggcgct
     3481 gcattccgga tgctgcaggg cgtaatcccg cagccggtaa taatggaccg tcatggcata
     3541 acactccgta cgacaggcgt gatgactgta cgtcatcaga caggcggcaa tgccggcggc
     3601 ttccgggctc atttcagcgc ggttaccgtt catggcattg aacagtaccc atgtttcgtc
     3661 attatcgtca tccggttcag gggccataaa tgcgccaccg ttgttcaggg tgtacagatt
     3721 ccagatacca ccgcagtagt cttcacacag acggtccatc cagccgaaga tacggggctc
     3781 cagggtcacc cactgtggaa tgaggccaaa atgctgcggc cagaagctga tgcgctgttc
     3841 atcagggact atggtggcaa ccagttgttg caggccagtc tccggtgcac aggccgaaga
     3901 ggtggtgttc tgagacaatg ttttcatgta tgaaatcctt gtaatgtgaa taaatgagag
     3961 ggagtaaagg tcgggagctg gcaacatcac gttcatcact gaccgtgaga cccggtaata
     4021 tcagggatga atgatattcg gctgaaattc cggacagctg tacgcgtaac cgttccggtc
     4081 acagacggat ggtcagatgc cattgcctgt actcagtctg cacaggctga gctctccgga
     4141 acggtaaagc aggcgggtac accaggtgca caggtcatca tcagaaatgg aacgacaccc
     4201 cggcagcgcc tccggggcgt cgggtgattc tggtacctga atcacgcctt cagggacagg
     4261 ctgcatcagc gcaggctctc cagcagcgtt tctgccatca cccacaacgc ccggttgagc
     4321 ttaatatcgg tgtcgatgct gtgaatggca cgggtgtgga tacgttttcc ttttgcactg
     4381 cgaccggaaa tcccgccttt cagcatattc tcctggatgg tctgataagc actccacagg
     4441 tccttaccgt aatcctcccg gcgtcgtggc gtcagaatgt cggcggtagt gacgggctga
     4501 tgttcgtcac cataacggta agtcagtgcc gcctgtgcca gcgcctggcg tgccggtggc
     4561 ggcaggacca gcgactgcat ggcatcacgc ttctcctcta tccggtcaaa cacgcccacc
     4621 acctcgtaag ccccttcaat gactctgtcc accacatttc cccggtgcgg aacacgcact
     4681 tcccccagag actgaccaca gacgcatccg ttctggcaga cgaacctgaa gtaacccggc
     4741 agcatctggt agctggaggt accgtcatgg gagttgagca gaataatttc agggacatgt
     4801 tctccgttta tctctccggc ccgccgcaga cgcagcatgt gttttgtgta tccccggcgg
     4861 cccgggtcgc gcacacgggt ctggcaggcg aagaatggct ggaagccttc ccgctgcaga
     4921 ctttccagta cggtgatggt ggggatgtac gtatagttct ggcttcggga tgtgtgttta
     4981 tcttctccga aaatccccgg cacatggtgc atcagctctt catgtgtcag cggacggtca
     5041 cggcgtatct ggttcgcata accaaaacga ctgactaatc gcatactttg ctccttatcg
     5101 gtggttaaga tttactggtg caataaatga aaaaagccgc atctcccgga gaagacgcgg
     5161 cctgacgggt gaaatgaata aggtttatgg tctgagaagc ctttcactgg cgagccacgc
     5221 attaagcagc tttccggtaa caatggccga ggctgtattt acctttgata ttaataatgt
     5281 gcagagaaac gcatggttta ctcctcagat tttaagtttg tccagagcca caacgacagg
     5341 ttttccggat gtgtcaatga ctcctaccgt cggtacgcca tttttgattt cgaagaacgg
     5401 tttgttgcta cctgagcaca gttgattgtc cccgtattca atcatttgtg ctttcccggt
     5461 tttcaggtta actaccgaga cagtgtagtc gtagcaccag gcaccgccac gttcaagcac
     5521 gaaaacatag ctgttatctt tatagttgaa ctgattcttt gtctggcaat tcataccgtc
     5581 aatttcggta caggaaaacg cttctgtagt attaacgtat ttgatgttat ttggcagttt
     5641 tacttgtttc gcagtagcga agacagaaaa cagaatagtt acagcaacga gtaaagtttt
     5701 gataaacgtt ttcatgatag actccttaaa ttagttaacc gcagctacgt agtttttcac
     5761 cagataggcg aattcatgga cgccaatttc gccgaaccaa accaggtagg ccgggtcatc
     5821 taaaccatag tagcggtagc caacgacttc atgggttttt gggttataga gtgttacgat
     5881 aaagtcccag ccgcaagcgt gggctttgca accgccacct tctacccagg tcgttccgtt
     5941 aatgtttaca ggtgttacac ttccgccagg gccatccata tacccgcctt taccgaacat
     6001 gccgctcagt ccgaccggtt ttaatacttt gtcaagcgca gctttgtagg cagcgtcacc
     6061 ttcgtacaac tcgtacgttg ttggacacgc tccaggttgt tcggtccagg cttccgggtt
     6121 acagctaagg gcttgtttca cggtagccgc ttctgcgtta aagccagtaa acagaaccgc
     6181 cagaaataac atcagggtat gtaaacgttt cattgtaaat attcttccgt tgtaaatggc
     6241 gctggaaaat ttctcatcag tgaaaccttc cggaagtttc caccccttgt atgatgtata
     6301 tcgccagtat tttatttcat cccggctgta atttttatat tccccggcac gtgcgataac
     6361 gctgaccaga gatataaggc tcattaataa ctttacaggt aatttcataa ttaaatttcc
     6421 agtgagtgaa ttgacaggt
//
LOCUS       DAZUCE010000261         6412 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZUCE010000261 DAZUCE010000000
VERSION     DAZUCE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.261"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..209)
                     /locus_tag="ACHY03_004482"
     CDS             complement(<1..209)
                     /locus_tag="ACHY03_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS3907636.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHY03_004483"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHY03_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFS3907637.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHY03_004484"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHY03_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFS3907638.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHY03_004485"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHY03_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFS3907639.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHY03_004486"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHY03_004486"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFS3907640.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHY03_004487"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHY03_004487"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFS3907641.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHY03_004488"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHY03_004488"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFS3907642.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANHLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="ACHY03_004489"
     CDS             complement(4598..5320)
                     /locus_tag="ACHY03_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3907643.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHY03_004490"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHY03_004490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFS3907644.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
BASE COUNT         1562 a         1654 c         1534 g         1662 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccgttt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca agtggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagtggaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaagg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt tt
//
LOCUS       DAZUCE010000262         6399 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZUCE010000262 DAZUCE010000000
VERSION     DAZUCE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.262"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            5..967
                     /gene="iaaA"
                     /locus_tag="ACHY03_004491"
     CDS             5..967
                     /gene="iaaA"
                     /locus_tag="ACHY03_004491"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFS3907645.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDAVAT"
     gene            957..2828
                     /gene="gsiA"
                     /locus_tag="ACHY03_004492"
     CDS             957..2828
                     /gene="gsiA"
                     /locus_tag="ACHY03_004492"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFS3907646.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDQMLLRRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            2848..4386
                     /gene="gsiB"
                     /locus_tag="ACHY03_004493"
     CDS             2848..4386
                     /gene="gsiB"
                     /locus_tag="ACHY03_004493"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFS3907647.1"
                     /translation="MARAVYRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            4404..5324
                     /gene="gsiC"
                     /locus_tag="ACHY03_004494"
     CDS             4404..5324
                     /gene="gsiC"
                     /locus_tag="ACHY03_004494"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFS3907648.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            5327..6238
                     /gene="gsiD"
                     /locus_tag="ACHY03_004495"
     CDS             5327..6238
                     /gene="gsiD"
                     /locus_tag="ACHY03_004495"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFS3907649.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
BASE COUNT         1406 a         1542 c         1882 g         1569 t
ORIGIN      
        1 tactatgggc aaagcagtca ttgcaattca tggtggcgca ggtgcaatta gccgcgcgca
       61 gatgagtctg caacaggaat tacgctacat cgaggcgttg tctgccattg ttgaaaccgg
      121 gcagaaaatg ctggaagcgg gcgaaagtgc gctggatgtg gtgacggaag cggtgcgtct
      181 gctggaagag tgtccactgt ttaacgccgg aattggcgcc gtctttacgc gtgatgaaac
      241 gcatgaactg gacgcctgtg tgatggatgg taacaccctg aaagccggtg cggtggcggg
      301 cgttagtcat ctgcgtaatc cggttcttgc cgcccggctg gtgatggagc aaagcccgca
      361 tgtgatgatg attggcgaag gggcagaaaa ttttgcgttt gctcatggca tggagcgcgt
      421 ctcaccggag attttctcca cgcctttgcg ttatgaacaa ctaatggcag cgcgcgagga
      481 aggggcaaca gtcctcgacc atagcggtgc gccactggat gaaaaacaga aaatgggcac
      541 cgtgggggcc gtggcgttgg atttagacgg caatctggcg gcagccacgt ccacgggcgg
      601 aatgaccaat aaattacccg gacgagttgg cgatagcccc ttagtgggtg ccggatgcta
      661 cgccaataac gccagtgtgg cggtttcttg taccggcacg ggcgaagtct tcatccgcgc
      721 gctggcggca tatgacatcg ccgcgttaat ggattacggc ggattaagtc tcgcggaagc
      781 ctgcgagcgg gtagtaatgg aaaaactccc tgcgcttggc ggtagcggtg gcttaatcgc
      841 tatcgaccat gaagggaatg tcgcgctacc gtttaacacc gaaggaatgt atcgcgcctg
      901 gggctacgca ggcgatacgc caaccaccgg tatctaccgt gaaaaagggg acgccgttgc
      961 cacatagtga tgaacttgat gccggtaatg tgctggcggt tgaaaatctt aatattgcct
     1021 ttatgcagga ccagcagaaa atagctgcgg tccgcaatct ctcttttagc ctgcaacgcg
     1081 gtgagacgct ggcaattgtt ggcgaatccg gctctggtaa gtcagtgact gcgctggcat
     1141 tgatgcgctt gttggaacag gcgggcggtt tagtgcagtg cgatcaaatg ctgttgcggc
     1201 ggcgcagtcg tgaagtgatt gaacttagcg agcagagcgc tgcacaaatg cgccatgtgc
     1261 gcggtgcgga tatggcgatg atatttcagg agccgatgac atcgctgaac ccagtattta
     1321 ctgtgggtga acagattgcc gaatcaattc gtctgcatca aaacgccagt cgtgaagaag
     1381 cgatggtcga ggcgaagcgg atgctggatc aggtacgcat tccggaggca caaaccattc
     1441 tttcacgtta tccgcatcaa ctctctggcg ggatgcgcca gcgagtgatg attgcgatgg
     1501 cgctgtcatg ccgcccggcg gtgctgattg ccgatgagcc aaccaccgcg ctggatgtca
     1561 ctattcaggc gcagatcctg caattaatca aagtattgca aaaagagatg tcgatgggcg
     1621 ttatctttat cactcacgat atgggcgtgg tggcagagat tgccgatcgg gtactggtga
     1681 tgtatcaggg cgaggcggtg gaaacgggta ccgtcgaaca gatttttcat gcaccgcaac
     1741 atccttacac ccgtgcgctg ttagctgctg ttccgcaact tggtgcgatg aaagggttag
     1801 attatccccg acgtttccca ttgatatcgc ttgaacatcc agcgaaacag gcccccccca
     1861 tcgagcagaa aacggtggtg gatggtgaac ctgttttacg ggtgcgtaat ctggtcaccc
     1921 gtttcccttt gcgcagcggt ttgttgaatc gcgtaacgcg ggaagtgcat gccgttgaga
     1981 aagtcagttt tgatctctgg cctggcgaaa cgctatcgct ggtgggcgag tctggcagcg
     2041 gtaaatccac taccgggcgg gcgttgctgc gcctggtcga atcgcagggc ggcgaaatta
     2101 tctttaacgg tcagcgaatc gataccttgt cacccggcaa acttcaggca ttacgccgcg
     2161 atattcagtt tatttttcag gacccttacg cttcgctgga cccacgtcag accatcggtg
     2221 attcgattat cgaaccgctg cgtgtacacg gtttattgcc aggtaaagac gcggctgcac
     2281 gcgttgcgtg gttgctggag cgcgtgggcc tgttacctga acatgcctgg cgttacccgc
     2341 atgagttttc cggcggtcag cgccagcgca tctgcattgc tcgcgcgttg gcattgaatc
     2401 caaaagtgat cattgccgac gaagccgttt cggcgctgga tgtttcaatt cgcgggcaga
     2461 ttatcaactt gttgctcgat ctccagcgtg atttcggcat tgcgtatctg tttatctccc
     2521 acgatatggc tgtggtagag cggattagtc atcgtgtggc ggtgatgtat ctcgggcaaa
     2581 ttgttgaaat tggtccacgg cgcgcggtct tcgaaaaccc gcagcatcct tatacgcgta
     2641 aattactggc ggcagttccg gtcgctgaac cgtcccgaca acgaccgcag cgtgtactgc
     2701 tgtcggacga tcttcccagc aatattcatc tgcgtggcga agaggtggca gccgtctcgt
     2761 tgcaatgcgt cgggccgggg cattacgtcg cacaaccaca atcagaatac gcattcatgc
     2821 gtagataaca ttcaggcgga gaataaaatg gcaagagctg tataccgtag tgggttagtg
     2881 gcgctgggca ttgcgacagc gttgatggca tcttgtgcat tcgctgccaa agatgtggtg
     2941 gtggcggtag gatcgaactt caccacgctc gatccgtatg acgcaaatga cacgttatct
     3001 caggccgtag cgaaatcgtt ttaccagggg ctgttcggtc tggataaaga gatgaaactg
     3061 aaaaacgtgc tggcggagag ttataccgtt tccgatgacg gcattactta caccgtgaaa
     3121 ttgcgggaag gaattaaatt ccaggatggc accgatttca acgccgcagc ggtgaaagcg
     3181 aatctggacc gggccagcga tccggcaaat catcttaaac gctataacct gtataagaat
     3241 attgctaaaa cggaagcgat cgatccgaca acggtaaaga ttaccctcaa acagccgttc
     3301 tcagcgttta ttaatattct tgcccatccg gcgaccgcga tgatttcacc ggcagcgctg
     3361 gaaaaatatg gcaaggagat tggttttcat ccggtgggaa ccggaccgta tgaactggat
     3421 acctggaatc agaccgattt tgtgaaggtg aaaaaattcg cgggttactg gcagccagga
     3481 ttgcccaaac tggacagcat aacctggcgt ccggtggcgg ataacaacac ccgcgcggca
     3541 atgctgcaaa ccggtgaagc gcagtttgct ttccccattc cttacgagca ggccgcactg
     3601 ctggagaaaa acaaaaatat cgagttgatg gccagtccgt caattatgca gcgttatatc
     3661 agtatgaacg tgacgcaaaa gccattcgat aacccgaagg tccgtgaggc gctgaattac
     3721 gccattaacc gtccggcgct ggtgaaagtg gccttcgcgg gctatgcaac gccagctact
     3781 ggtgtggtac cgccgagtat cgcctacgcg caaagttata aaccgtggcc ttacgatcca
     3841 gtgaaagcgc gcgaattact gaaagaggcg ggatatccca acggtttcag taccacgctg
     3901 tggtcgtcac ataaccacag caccgcgcag aaagtgctgc aatttaccca gcagcagtta
     3961 gcgcaggtcg ggattaaagc ccaggtgact gcgatggatg ccggacagcg ggcggcagaa
     4021 gttgaaggta aagggcaaaa agagagcggc gtgcggatgt tctacactgg ctggtcggct
     4081 tcaaccggcg aagccgactg ggcactatcg ccgttgtttg cctcgcaaaa ctggccaccg
     4141 acgctgttta ataccgcgtt ttacagcaat aaacaggtgg atgacttcct ggctcaggca
     4201 ctgaaaacta atgatccggc ggaaaagacc cgcttgtata aggcggcgca ggatatcatc
     4261 tggcaagagt cgccgtggat cccgctggtg gtagaaaaac tggtgtcggc acacagtaaa
     4321 aacctgaccg gtttttggat catgccagac accggcttca gctttgaaga cgcggatttg
     4381 caataagcaa cgcagggagt ggaatgctta attacgttat caaacgatta ctggggttga
     4441 ttccgacgct gtttatcgtc tcggtgctgg tgtttttatt tgtccatatg ctgcccggcg
     4501 atccggcgcg attgattgcc gggcccgaag ctgatgcgca ggttatagaa ctggtgcgtc
     4561 agcagctggg gttggatcag ccgctgtatc accagttctg gcactatatc agcaatgctg
     4621 tgcaggggga ttttggcctg tcgatggtgt cgcgtcgtcc ggttgccgat gagattgcca
     4681 gccgctttat gccaacgctg tggctgacca taaccagtat ggtctgggcg gtgatatttg
     4741 gtatggcggc gggaattatc gccgccgtct ggcgtaaccg ttggccggat cgattgagta
     4801 tgaccattgc ggtgtcgggg atctcgtttc cggcatttgc gctggggatg cttttaattc
     4861 aggtattctc cgttgaactg ggctggctgc ctaccgtggg agcagacagt tggcagcact
     4921 acattttacc ctccctgacg ctcggcgcgg cagtggccgc cgtgatggcg cgctttaccc
     4981 gcgcgtcgtt tgtcgacgtt ttaagcgaag attatatgcg taccgcgagg gcgaaagggg
     5041 tgagcgaaac ctgggttgtc ctcaaacacg ggctacgcaa cgcgatgatc ccggtagtga
     5101 ccatgatggg cttacagttt ggctttttgc tcggtggttc catcgtggtg gagaaagttt
     5161 tcaactggcc cggacttgga cgcttactcg ttgactccgt agaaatgcgc gattacccgg
     5221 tgattcaggc ggaaattctg cttttctcgc tggaatttat tcttatcaac ttagtggtgg
     5281 atgtgcttta cgccgccatt aacccggcta tcaggtacaa gtaaggatgc gactatttaa
     5341 ctggcgacgt caggcggtgt taaacgccat gccactggtc aaacctgacc aggtacgtac
     5401 accgtggcat gaattctggc gacgatttcg ccgtcagcat atggcgatga ccgccgcatt
     5461 gttcgttatt ttattgattg tggtggccat ttttgcacgc tggatcgctc cctatgacgc
     5521 cgaaaattat tttgattatg acaatctgaa taacggacct tctttgcagc actggtttgg
     5581 cgtcgattca ctggggcgcg acattttcag ccgagtcctg gttggtgcgc aaatctcgct
     5641 ggcggcgggc gtgtttgccg tgtttatcgg tgcggcgata gggacgttgc tgggcttgct
     5701 ggctggatat tatgaaggct ggtgggatcg gctgatcatg cgcatttgcg atgtgctgtt
     5761 tgccttcccg ggtattttac tggcgatcgc tgttgttgcg gtgttgggaa gcggcattgc
     5821 taacgtgatt attgcagtcg ccattttttc catccctgcg tttgcccgcc tggtgcgcgg
     5881 caacacgctg gtgttgaaac agcaaacctt tattgagtca gcacgcagta ttggtgccag
     5941 cgatatgacc attttgttgc gtcatatcct gcctgggacc gtctcttcta tcgtggtgtt
     6001 tttcaccatg cgcattggta cctcgattat ctctgccgcc agcctgtcat ttctcggcct
     6061 cggtgcgcag ccgccgacac cagagtgggg agcaatgctc aatgaggctc gagcggatat
     6121 ggttatcgcg ccgcatgtcg ctgtttttcc ggccctggct atttttctga ctgtactggc
     6181 gttcaatttg ttgggcgatg gtttacgcga tgcgctggat ccgaaaatta aaggatagtt
     6241 acgtttgaat attgcttgaa agagtaatca cctcacagga aattattgcc ctaagcaagt
     6301 gttgtaactt tctgctgatt ttgtagaatc gggtaatttg gttaaatagc cgcagcaagg
     6361 gactgttttt gcagcggcac agcgttcaga cagttattt
//
LOCUS       DAZUCE010000263         6393 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZUCE010000263 DAZUCE010000000
VERSION     DAZUCE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.263"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..330
                     /locus_tag="ACHY03_004496"
     CDS             <1..330
                     /locus_tag="ACHY03_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3907650.1"
                     /translation="AKQTITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQI
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(331..1488)
                     /gene="ampH"
                     /locus_tag="ACHY03_004497"
     CDS             complement(331..1488)
                     /gene="ampH"
                     /locus_tag="ACHY03_004497"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFS3907651.1"
                     /translation="MKRSLFFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            1840..3060
                     /gene="sbmA"
                     /locus_tag="ACHY03_004498"
     CDS             1840..3060
                     /gene="sbmA"
                     /locus_tag="ACHY03_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFS3907652.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            3073..4167
                     /gene="yaiW"
                     /locus_tag="ACHY03_004499"
     CDS             3073..4167
                     /gene="yaiW"
                     /locus_tag="ACHY03_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFS3907653.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(4226..4534)
                     /locus_tag="ACHY03_004500"
     CDS             complement(4226..4534)
                     /locus_tag="ACHY03_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFS3907654.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            4794..5006
                     /locus_tag="ACHY03_004501"
     CDS             4794..5006
                     /locus_tag="ACHY03_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFS3907655.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(5030..6124)
                     /gene="ddlA"
                     /locus_tag="ACHY03_004502"
     CDS             complement(5030..6124)
                     /gene="ddlA"
                     /locus_tag="ACHY03_004502"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFS3907656.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            6202..>6393
                     /locus_tag="ACHY03_004503"
     CDS             6202..>6393
                     /locus_tag="ACHY03_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907657.1"
                     /translation="MLQSSADKACNVLMKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIY"
BASE COUNT         1576 a         1563 c         1645 g         1609 t
ORIGIN      
        1 gccaaacaaa ccattacgct tattgaacaa aatcaactct ggcgagacgc tttttactgg
       61 ttagcctggc aaaatagaat tctggaatta cgcgacgtgc agctcattgg gcataattcc
      121 tacgaacaaa tccgcgcaac gttattatca atgattgact ggaatgaaga attgcgatca
      181 cgtattggtg tgatgaatta tatccatcaa cgtacacgca tatcgcgttc tgtcgtcgca
      241 gaagttctcg ctgctttgcg taaaggcggc tatatcgaaa tgaataaagg caaactggtc
      301 gctatcaacc gtttgccttc agagtattaa tcaggacgcg gggataacca acggtttatt
      361 cccgcttagc tcggtcacca ggtcattgat gccatcgctc atatttttaa agcgcgtcaa
      421 cggcgaacga gtgaccacca caaacgcgcc aatattcttc tgcgggatca tcgccatata
      481 ggtaatgaaa ccgccaccac cgcctgtctt ctgaataatc cccggacgac cctctttcgg
      541 ggccatatat acccagccta aaccaagcgc atccgctttg ccggggacat ccatgccaat
      601 cactttcgta aactgcgcgc gctgataaat caacgtctgc atgcgatctg cctggttact
      661 gcgctgataa aaatcagatg acaaatactg ctgcatccag cgcatcatat cgccaggcgt
      721 ggaatagacg ccgccgctgc caattgccgc cagcgtgtta ttgcatggac tggcaccgcg
      781 ctctgcaacc attaaacggc ggcactgatc cggtgagggg gtgtaggtgg tgtctttcat
      841 ccccaacgga cgggtaatct gctcttcaaa caactgggta taaggcttgc cggacgcatt
      901 cgccagtgca tctgccagca ggtcgaacgc aaggttagag tacgccgcct gactgcctgg
      961 cgcggctttc agcttcgccg tagaaaggta tttccagcgt tgctcgcgcg ttggccagac
     1021 aaaaaccgga cgatgtgccg cgccaccggg ctgttcacgg ggcagggcgc tggtatgggt
     1081 tgccagattg accagcgtaa tgggcgttcc attgtaggtt ggcacgcgtg cgcctggcgg
     1141 agcatattta cttagcggat cgtttaactt cacggtcccc tggtcgagca atttcaccag
     1201 catctcactg gtcattagct tggtgaggga agcaatacgc acgaccgaat ccagctgcgg
     1261 gcgaacgtta ttaccaggtc gcgtttcgcc ataactgcga aagacgcgct ggttaccgtc
     1321 gataacaacc agcgccatcc ccgtagcacc gctgccgtaa aaaatatgat cggcataacg
     1381 atcaacaata tcagaagcga actccggttc agtgatcggc tgtgccgcat ggacggaggt
     1441 caatgacgcc gcacacagca cggcagaaaa aaacagacta cgtttcaacg gtggtgtcca
     1501 taaattgaat gaaggtaact gagcgtattt atactactca ttgatggatt gcaaagggcg
     1561 aattagtgca ataatagtgt taaaaacgta acggcgcgta aacacgatct ttttcctgct
     1621 caccaatgcc tgcatttgtg atcctgcgcg tgttaatccg cttatgtaaa caatctttgg
     1681 tttgtgaaat ccgtcgaaac aattcttatg gtcaggctgg cgaactaagc gccttgctat
     1741 gggtcacaat gggcgatttc atttgactga cgctttgtag cggtcatgcg gttaatacac
     1801 agcagttgtt aaaacgataa gaagttagca ggagtgcata tgtttaagtc ttttttccca
     1861 aagccgggaa cgttttttct ctcggccttc gtttgggcat tgattgccgt tatcttctgg
     1921 caagccggtg ggggtgactg ggtggcgcgt atcaccggcg cttccgggca gatcccgatt
     1981 agcgccgcgc gtttctggtc gttggatttc ctgatttttt acgcttacta cattgtttgc
     2041 gtaggacttt ttgcattgtt ctggtttatc tacagcccgc accgttggca atactggtca
     2101 atactcggta ctgcactgat catcttcgtc acctggtttt tggtggaagt cggggttgcc
     2161 gtcaacgcct ggtatgcacc gttctatgat ctgattcaaa ccgcgctaag ttcgccgcat
     2221 aaagtcacta tcgaacaatt ttaccgcgaa gtgggcgtct ttctggggat tgcgctgatc
     2281 gcggtagtga tcagtgtgct gaataacttc tttgtcagtc actacgtgtt ccgctggcgt
     2341 acggcgatga acgaatatta catggcgaac tggcaacaac tgcgtcatat cgaaggggcc
     2401 gcacagcgtg tgcaggaaga caccatgcgt tttgcttcaa cgctggagaa tatgggtgtc
     2461 agttttatca acgccatcat gacgttgatc gccttcctgc cggtgctggt aacgctctcc
     2521 gcgcatgtgc cggagctgcc gattatcggg cacattccgt atggtctggt gattgccgct
     2581 atcgtctggt cgctgatggg gaccggattg ctggcagtgg tagggatcaa actgccgggg
     2641 ctggagttta aaaaccagcg agtagaggct gcctaccgta aagagctggt ttatggtgaa
     2701 gacgatgcca cgcgcgcgac gccgcctacg gtacgcgagc tgtttagcgc cgtacggaaa
     2761 aactatttcc gcctctattt tcactatatg tatttcaaca tcgcccgcat tctctatttg
     2821 caggtcgata acgttttcgg tttgttcttg ctgtttccgt caattgttgc cggtacgatt
     2881 acgctcggcc tgatgacgca gattaccaac gtttttggtc aggttcgcgg tgcttttcag
     2941 tacctgatta actcatggac cacactggtt gagttgatgt ctatctacaa acgtctgcgc
     3001 agctttgaac atgagctgga tggtgacaaa attcaggaag taacccatac cttgagctaa
     3061 aaaggagaag taatgtcgcg cgtcaatcat ctatcttcgc tgtcattgtt ggcggttctg
     3121 gttttagcgg ggtgtagtag tcaggctccg caaccgttga aaaagggcga aaaagcgata
     3181 gatgtagcga gcgtcgtgcg gcagaagatg cctgcgagcg tgaaagaccg cgatgcctgg
     3241 gcgaaagatc tcgccaccac ctttgaaagt caggggctgg caccgacgct ggaaaacgtc
     3301 tgctcggtgc tggcggtggc gcagcaggag tcaaattatc aggcagatcc ggccgttccg
     3361 ggcttaagca agatcgcctg gcaagagatt gaccgtcgtg ctgaacggat gcacattcct
     3421 ccttttctgg ttcataccgc gctaaaaatc aaatcgccaa acggcaaaag ttatagcgaa
     3481 cggttagatt cggtgcgtac agaaaagcaa ttgagcgcga tttttgacga tctgatcagc
     3541 atggtgccaa tggggcagac gctgtttggt tcgcttaatc cggtgcgcac cggtgggccg
     3601 atgcaggtca gcattgcttt tgccgaacag cataccaaag ggtatccgtg gaaaatggac
     3661 ggtacagtcc gtcaggaagt ctttagtcgc cgcggcgggt tgtggtttgg tacttaccat
     3721 ttactgaatt atcccgccag ttatagcgca ccgatatacc gttttgctga ttttaacgct
     3781 ggctggtacg ccagccgtaa tgccgcgttt cagaacgcgg tcagtaaggc cagcggcgtg
     3841 aagctggcac tggacggtga tttaattcgt tacgacagta aagaacccgg gaaaaccgaa
     3901 ctggcaacgc gcaaactggc ggcaaaactg gggatgagcg acagcgaaat tcgtcgtcag
     3961 ctagagaaag gtgacagctt ctcttttgag gagacggcgc tgtacaagaa agtttatcaa
     4021 cttgccgaag cgaaaacggg taaatcactc ccccgcgaaa tgttgcctgg cattcaactg
     4081 gaaagcccga aaatcacgcg caacctgact acggcctggt ttgcgaagcg cgtagacgaa
     4141 cggcgggcgc gttgtatgaa acagtgatca aacaggaatg tcaggccaga taaggcgttt
     4201 taggccgtat ctggcaacgt aaatcttact ggcggcgatg tcgccagtgc agccatagcg
     4261 ccaccactgc aaaaatgagg cagcctacta aaaacgggat cagcccgaaa atggtgccga
     4321 cacctaaacc aatttccacc cgtgggcgac ctgtttcctg gacctgcatt aaacgctcat
     4381 agacgccggg cgcatgcact aacaggttca gcaattgcgc cccagcccaa aagcaaaaca
     4441 gcacaaacaa cgcataggca atgttgccag gcgttgaaga ggcattgcga tattttccgc
     4501 taaacagcga ttttgacagg gtgaaatcag ccataaaaac ctccggaagt catctgcttc
     4561 tcttctggtg tgataccagc ggcttatggc aagtctgaca ggtcatttag attgtcaata
     4621 tcagaatcat ggtaaattga tgttgggaat attcccgaag cgtgaatctt cgttgcgagt
     4681 cacaaaatag caactttaag ttaaatttat taataattgc gaaaatgttg tctacaccac
     4741 agacggttgt tgcagaatat gcaaggatgt tgtttttcgt taacggagca gccatgaatc
     4801 tgcctgtaaa aatccgccgt gactggcact actatgcgtt cgccattggc cttatattca
     4861 ttcttaatgg cgtggtgggg ttactgggat ttgaagcaaa aggttggcag acctatgccg
     4921 tcggtctggt gacgtgggtg attagtttct ggctggcggg gttgattatt cgtcgtcgcg
     4981 atgaagaaac tgaaaacgcc caataagaat attatcgggc gttaaaatat tacattgtgg
     5041 ttttcaatgc gttatccgca gcgtgacgct ccagcgccag ctcaatcaaa cgtgtgatca
     5101 gatcggtgta acccagaccg ctggcttgcc acagcttcgg atacatactg atgttggtga
     5161 agccaggcag tgtgttgatc tcgttgatca ccacttcgtt ctctggggtt aaaaacacgt
     5221 ctacacgcgc catgcctgcg catcccaacg tctgataagc ctgaacggca atcgcccgga
     5281 tcttatcgtt gatttctggc gcaatggctg ccggaactac cactttcgcg ccatcttcgt
     5341 caatgtactt ggtgtcgtag gcatagaaat cgctggtgag tacgatctcg ccacaggtgc
     5401 tggcttgcgg attgtcgttg cccagaactg cgcattcgat ctcacgacct ttgatccctt
     5461 gctcaacgat cactttatga tcgaactcga acgccagatc gacggcaatt gcgtactgtt
     5521 cttcactggt tactttgctg acaccaacag aagagccctg attagccggt tttacaaaca
     5581 gtggtaaccc cagtttagac tccacttcgg caaaactgat gttgtgacga ttagcgcgcg
     5641 tcagggtaat aaatggcgca atgttcagcc cggcatcgcg tagcagacgt ttggtgacat
     5701 ctttatccat acaggctgct gaagccagaa catcagaacc tacaaacggt aaattggcga
     5761 cccgcagcat tccctgcaag gaaccatctt cgcccagcgt accgtggaca atcgggaaaa
     5821 tgacatccac cgtcggcaac ggctgaccgt tttgcgcgtc gataagctga tgctcatgtt
     5881 tacctggcac ctgggcgagg ctggtcgccg aagggcgcaa cgcaatatgg gcaggatcgt
     5941 ctgcatttag cagataattg ctggcatcgc tgacgtgcca ttgcccttgt ttatcaatgc
     6001 ccagcagcac aacgtcgaag cgacttttat caatggcatc gacaatgttt tttgccgatt
     6061 gcagagacac ttcatgttcc gctgatttac caccaaaaac gattcctacc cgcagttttt
     6121 ccatcttaaa aacctatccc gtctaacaca aagtgcatac attaccacga caaaacgggg
     6181 gattcgcggc cttctgaaag attgttgcaa tcttctgctg acaaagcgtg caacgtactg
     6241 atgaagaaag tgcgttatct caaagatgtg cgcaagatca caaaaatgat gaacgggaag
     6301 ctaatttatt cctggcttaa atggccatgc ggtgagtttt tttctcttaa ttataagtta
     6361 acgaagagaa tatatttcat aacttttatt tat
//
LOCUS       DAZUCE010000264         6372 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZUCE010000264 DAZUCE010000000
VERSION     DAZUCE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.264"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            247..462
                     /locus_tag="ACHY03_004504"
     CDS             247..462
                     /locus_tag="ACHY03_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3907658.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            636..893
                     /locus_tag="ACHY03_004505"
     CDS             636..893
                     /locus_tag="ACHY03_004505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907659.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            875..1066
                     /locus_tag="ACHY03_004506"
     CDS             875..1066
                     /locus_tag="ACHY03_004506"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3907660.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            1208..2227
                     /locus_tag="ACHY03_004507"
     CDS             1208..2227
                     /locus_tag="ACHY03_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS3907661.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            2948..3163
                     /locus_tag="ACHY03_004508"
                     /pseudo
     CDS             2948..3163
                     /locus_tag="ACHY03_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3338..5308
                     /locus_tag="ACHY03_004509"
     CDS             3338..5308
                     /locus_tag="ACHY03_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFS3907662.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            5327..6118
                     /locus_tag="ACHY03_004510"
     CDS             5327..6118
                     /locus_tag="ACHY03_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFS3907663.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         1917 a         1404 c         1524 g         1527 t
ORIGIN      
        1 gtaaaaacac tcacaaggga acatagattc aatgcgttta tttagaaggt ctgtcttctt
       61 tttaatatta acttattaat tggtgctatt catataattc tttaaacagc gaacttatag
      121 ccaggattca atagatatag taacaggaac atcagtaagc gatgagatgg tcttccccct
      181 ccttcacgac aacctttaat tacgtcatga tggataaact ttaaccacaa aagtaagagg
      241 gataccatgt ctcaacagaa aatcaccggc atcgatctgg ctaaaacgaa cttttatctt
      301 ttcagtatca atgctcatgg caaacccgca ggaaggatca aactttctcg caatcaactg
      361 cttaactggc tggtgcagca gcctaaaatg actgttgcga tggaggctcg cgggcatccc
      421 atcactgggc cagggaaatc aggaaactcg accatgatgt aatatttctt ccggcgcaac
      481 atgttaaagc ttatcagcga cgccagaaaa atgattatga cgacgctcag gcaatagctg
      541 aggcctgcca acatggaact atacgccccg taccaataaa aactctggag taacaggacg
      601 tccagacttt tttgaaaatg cgccggttgg tttcgatgga aaggacacaa ctgataaatc
      661 acgttcgtgg acttcttgct gaatatggca ttgtgttcag taaaggcgca acagaattac
      721 gccaaaaatt accggcatta cttgaggatg cggaaaacga actcactgat accatgaaga
      781 cgttattgca tcggcagtat atacggttga ttacactaga taatgaactg gagtggtatg
      841 attctgaact gaaaaatatg tccgccaaga tcctatgtgc aaacgcttgt tagccattcc
      901 tagattcagc cctctggtta gtcaggcaat aaaaagctgg atgggggatg gaaaacagtt
      961 tagacgagga agagatgcat ctgcgactct gggactggtt ccccgacagt tcagcacagg
     1021 tgggaagcag gttcttctgg gtataacaaa atttggagaa atgtaatgaa cacatcccac
     1081 cccattaagt caggtgctct gtgatggatg aagggaaata ctggaaggtg tggagcctcg
     1141 cagatttccg gcatcatagt gcccaataca ggatgcataa agctccctca gactggagac
     1201 tccacaaatg aaatatacac cggttggcgt tgatatcgca aaacatctga ttcaggttca
     1261 tttcatcgac gagcacacag gtgaagtgat tgataagcaa ctacgtcgtc aggattttct
     1321 gatgttcttc tgtaatcggg aaccctgcct gattggtatg gaggcctgta gaggctctca
     1381 gcactggggg cgtgaactga caaagctggg gcataaagtc cggttgttac aggcccactt
     1441 tgttaaggca ttcgtcatgg gaaataagaa tgatgtgatg gatgcccggg ctatctggat
     1501 ggctgttcag cagccaggta aggacatcgc ggttaaaaca gaagaacaac agtcagtgct
     1561 ggttttgcac cgttcccgca gacaattggt taagttccgc accatgcaga ttaattctct
     1621 gcacggcacg ctcctagaat ttggtaaaac cattcacaaa ggtcgggcag cgatggagcg
     1681 agagctccct gaagcactgg agcggatgaa agagagactg ccgccgtatc tcattactgt
     1741 gctggaaaac cagtacaacc ggctgaatga gctggactcg ctgatagagg atattgaaaa
     1801 aaagcttacc agcgtggcga gacagaatga aacctgtaag cggttgctgg atattcccgg
     1861 cgttgggcca cttattgcaa cggcagcggt ggccaccatg agggaagcat cagcgtttaa
     1921 atcagggcga gagttcgcgg cttatgttgg tctggtgccc aaacagactg gctcaggagg
     1981 gaaaatccgc ctgctggaga taagcaaacg tggtaataca tatctgcgag ccttgtttat
     2041 tcatagcgca agggcggcag cattactgcc caaagaacct ggtccgtgga taaccgaact
     2101 gaaaaaacgt cgtccaacca gcgtggcaat cgtcgccatg gcaaacaaac tagcacgaac
     2161 agtatgggcg atagcagctc atggaagtaa atacgacaag aatcatgtca gtatcagatc
     2221 atattaatcg ctgataccga taaatattaa cttttaatga aaggatgaat actgaaaggt
     2281 aactctggcg gccatagtga tgacaacgac aggtaagacc gggactcact aaacctgaat
     2341 attgtgttga gctaaaagct cgctggagaa atgaggtgtg agtcagcgaa ttacataggg
     2401 gctcgcagct tcatggctgc aaaaaagccg gatataaagc tgcaacctac ccgtcactgt
     2461 caaggcaatg gatgccttgc aacgggatgt gttcatataa tcaatatgtc agatctatgc
     2521 tgatacaggg tgccagagcg gtattgttca gagcctcaga gaaaactgat tcacttcgtg
     2581 tgtgggttaa ttgtattcgg gaaaagcgtg gcttcaacca ggcagttgtt gctcttgcaa
     2641 ataaactcct tcggatcgcc tgggcaatta tcgcccgcaa tgagatgtat ttgcccgcag
     2701 caaaacaaaa tcaataatct acgtaaaatc agtaggagta aagcttaccc tgaaattgca
     2761 aagaatgcag aatgatgaaa aacaggtcag accgacgtta cgaaaacctg caagatgagc
     2821 caggccagta aggccgagta agcgataagg tcggagcgcg cgaatgtctc atcaaggcca
     2881 gaagcaataa cttcatgaac aaaccggata tacgaaagta aacctgtccc tatcatagtt
     2941 gctgtactct tgcaatacgg gggaagacca tctcatcgct tacgaacaat tcgtgaaaac
     3001 gatgaagaaa gactacatcg cgtgcatgcc gaaatctaat gtaagaacgg cattgcataa
     3061 tcttgcagtg gagatcgggt attacaatga aaaccatccg catagtgcgt taggttatct
     3121 cccaccaagg gaatatcaac gtcagcgaat aacgtcacct tagatgcagt agttgtcagg
     3181 aaatagtgac tcaagggcag gcttaccata tacactctta gtggttcgac tgggtattag
     3241 aatagctatt aacagattag cactgaacac tgcgtaatag tggttgcaca aacaaaatgt
     3301 tatgttataa cattgctgta ttgacatata ttttcgaatg tatatgaacg taatcagaac
     3361 tgtcatttgt acattaatca tacttccggt ggggttacag gcagcgacaa gtcattcttc
     3421 tatggctaaa gatacaatca ccgttgtcgc gacaggaaat cagaacacgg tatttgaaac
     3481 gccgtcgatg gtcagtgtca tcacgaatga cacgccatgg agtcagaatg cggtcacatc
     3541 agccggtatg ctgaaaggtg ttgcgggtct cagccagact ggtgcaggac ggaccaatgg
     3601 gcagaccttt aatctacgcg gctatgacaa aagcggggta cttgttcttg ttgacggcgt
     3661 ccgtcaactc agtgacatgg caaaaagcag tggcacttat ctggatccgg cactcatcaa
     3721 acgcatcgaa gttgtccgcg gaccaaactc cagtctgtac ggcagtggcg ggctgggggg
     3781 ggtggtggac ttcagaactg ccgatgctgc agattttctt ccccccggag agacaaatgg
     3841 tgtaagtctg tggggaaata tcgccagtgg tgaccacagc acaggctcgg ggctcacctg
     3901 gttcggtaaa accggaaaaa cagatgcgct tctttctgtc attatgcgta aaagaggaaa
     3961 tatctatcag agtgatggtg agcgtgcacc taacaaggaa aaacctgcag ccctgtttgc
     4021 gaaaggctct gtcagtataa cagacagtaa caaagcaggt gccagcttgc gtctctacca
     4081 gaataacacc actgaaccgg gcaatcccac tctgacacat ggtgacaacg gcctgcgtga
     4141 cagaaaaaca gcacaaaatg acgtgcagtt ctggtaccag tactcccctg cggataacag
     4201 cctcatcaat gtaaagtcaa cgttatatct cagtgatatc actatcaaga caaacggtca
     4261 caacaaaaca gcagaatggc gaaacaacag aacctccggt gttaatgttg tcaacaggag
     4321 ccatactctg atttttccgg gagcccatca gttaagttat ggcgccgaat attaccgtca
     4381 gcagcagaag ccggaaggct ctgccacact atatccggaa ggaaacattg actttacatc
     4441 gttgtatttc caggatgaaa tgacaatgaa aagcaacccg gttaacatta tcgtcggttc
     4501 ccgttatgac cggtacaaga gcttcaatcc ccgagccgga aaactgaaag ccgaacgcct
     4561 gtcccccagg gcggcgattt cagtctcacc aacagactgg ctgatgatgt acggctccat
     4621 atcctctgca ttccgtgccc ccacaatggc agaaatgtac agggatgatg tacattttta
     4681 ccgcaagggt aaacccaatt actgggtacc taaccttaat ctgaaaccag aaaataacat
     4741 tacccgtgag attggcgcag gtattcaact ggacggcctg cttacagaca atgaccggct
     4801 gcagttgaaa ggcggatatt tcggaacgga tgccagaaac tatattgcca cgcgcgtgga
     4861 tatgaaacgg atgcgttctt attcttataa tgtatcccgg gcccgtatct ggggatggga
     4921 tatgcagggt aattaccagt ctgattattt tgactggatg ctttcttata accgcacgga
     4981 aagtatggat gccagtagca gggaatggct gggctccggc aatcctgaca cactcatcag
     5041 tgacatcagt atacctgttg gtcatggagg agtttctgcc ggatggcgtg ctgaactttc
     5101 ggcagcagcc acgcatgtga aaaaaggcga tccccatcag gctggctatg ccgtacactc
     5161 cttttcactg tcttataagc ctgtaagtgt taaaggcttt gaggcatcag tgactctgga
     5221 taatgccttc aacaagcttg ccatgaatgg caaaggtgtg ccgctttcag gcagaactgt
     5281 cagtctttat acccgttatc agtggtaacc tcgcaggaaa taaatcatga tgaaaaatac
     5341 aggctatatc ttagctcttt gtctgacagc atcggagcat gccctggccc acgatgtctg
     5401 gattaccggt aaacaggcag agaacaacgt taccgcagag attggttatg gtcataattt
     5461 cccctcaaag ggcacaattc ctgacagaag gaatttcttt gaaaatcccc ggctttataa
     5521 cgggaaagag ataataacgc tgaagccaac gtccacggat tatgtctata aaactgagtc
     5581 tgcaggcaac ggtaatagct acgttctgtc aacgaaaatg aaaccgggat actggtcgag
     5641 aacctcgtca ggatggaagc ctgtcagtcg ggagggcaga aatgatgtag catactgtga
     5701 gtttgtgact aaatatgcaa aatcttttat tcctggtgaa cagcagatgc cagcacaact
     5761 ctatcagtct ccaacagggg atgagcttga aatcattcca ttatccgata tcagtcgttt
     5821 cggtgaagat gtgaagctga aagttctgta taaaacatcc ccgcttgccg gagctactct
     5881 ggagcttgac tctgtcagtt atctgaaatc atcccgtcat acccatgcag ctgagcataa
     5941 acattcggct cataaagcag aacttacctt tgtaagtaat gaggacggta tcatcactgt
     6001 accttccctt catgtcgggc aatggctggc taaagtcaaa aataagaaag tatttccgga
     6061 caagagtctg tgtgatgaaa ctgtcgatgt ggcaacctta agcttctccc gaaattaata
     6121 atactattcc tgccctgatt tatgggcagg gtattatcaa tacgagaata gcctaccgac
     6181 atccgtgtaa aaaaccacat aatgattttt catatccatt tccagatata cttatgtaaa
     6241 gatcacctcc aaaaaagcgt aatagtaata aaaatccctg aagcaccctg ttcaaaccag
     6301 aaggaataca taacaagaca ataaaatgtg aaatccatca cagcaaagaa ctctttgttt
     6361 ataatataat ga
//
LOCUS       DAZUCE010000265         6285 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZUCE010000265 DAZUCE010000000
VERSION     DAZUCE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.265"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            67..996
                     /gene="pfkB"
                     /locus_tag="ACHY03_004511"
     CDS             67..996
                     /gene="pfkB"
                     /locus_tag="ACHY03_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFS3907664.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVAAVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNKGTRLCSHDDTQKIYAYLSR"
     gene            1097..1387
                     /gene="ghoS"
                     /locus_tag="ACHY03_004512"
     CDS             1097..1387
                     /gene="ghoS"
                     /locus_tag="ACHY03_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFS3907665.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1493..2353
                     /locus_tag="ACHY03_004513"
     CDS             1493..2353
                     /locus_tag="ACHY03_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFS3907666.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2394..2930)
                     /locus_tag="ACHY03_004514"
     CDS             complement(2394..2930)
                     /locus_tag="ACHY03_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFS3907667.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3077..3745
                     /gene="hxpB"
                     /locus_tag="ACHY03_004515"
     CDS             3077..3745
                     /gene="hxpB"
                     /locus_tag="ACHY03_004515"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFS3907668.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3908..4498
                     /locus_tag="ACHY03_004516"
     CDS             3908..4498
                     /locus_tag="ACHY03_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFS3907669.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4631..6022
                     /gene="tcyP"
                     /locus_tag="ACHY03_004517"
     CDS             4631..6022
                     /gene="tcyP"
                     /locus_tag="ACHY03_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFS3907670.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(6026..>6285)
                     /gene="ydjO"
                     /locus_tag="ACHY03_004518"
     CDS             complement(6026..>6285)
                     /gene="ydjO"
                     /locus_tag="ACHY03_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFS3907671.1"
                     /translation="LNLPGMGSGILEDIMSIPDSSLRKRLGYEVLSFSLQAHSLSQEC
                     IDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1538 a         1554 c         1578 g         1615 t
ORIGIN      
        1 tattgttaat ttcttcactt tccgctgatt cggtgccaga ctgaaatcag cctataggag
       61 gaaatgatgg tacgtatcta tacgttgaca cttgcgccct ctctcgatag cgcaacaatt
      121 accccgcaaa tttatcccga aggaaaactg cgctgtaccg caccggtgtt cgaacccggg
      181 ggcggcggca tcaacgtcgc ccgcgccatt gcccatcttg gaggcagtgc cacagcgatc
      241 ttcccggcgg gtggcgcgac cggcgaacac ctggtttcac tgttggcgga tgaaaatgtc
      301 cccgtcgctg ctgtagaagc caaagactgg acccggcaga atttacacgt acatgtggaa
      361 gcaagcggtg agcagtatcg ttttgttatg ccaggcgcgg cattaaatga agatgagttt
      421 cgccagcttg aagagcaagt tctggaaatt gaatccgggg ccatcctggt cataagcgga
      481 agcctgccgc caggtgtgaa gctggaaaaa ttaacccaac tgatttccgc tgcgcaaaac
      541 caagggatcc gctgcatcat cgacagttct ggcgaagcgt taagtgcagc actggcgatt
      601 ggtaacatcg agttggttaa gcctaaccaa aaagaactca gtgcgctggt gaatcgcgaa
      661 ctcacccagc cagacgatgt ccgcaaagcc gcgcaagaaa tcgttaatag cggcaaggcc
      721 aaacgggttg tcgtttccct gggtccacaa ggagcgctgg gtgttgatag tgaaaactgc
      781 attcaggtgg tgccaccacc ggtgaaaagc cagagtaccg ttggcgctgg tgacagcatg
      841 gtcggcgcga tgacactgaa actggcagaa aatgcctctc ttgaagagat ggttcgtttt
      901 ggcgtagctg cggggagtgc agccacactc aataagggaa cacgtctgtg ctcccatgac
      961 gatacgcaaa aaatttacgc ttacctttcc cgctaacaaa aacattcccc cagcattggg
     1021 ggaatcatca ccaacctgtc ggcaacgcgt ttctccgact atgctcaaaa gtcatgtgat
     1081 aacaaagggg tgaactatgg ccagtggcga tcttgtccgt tatgtcataa ccgtcatgtt
     1141 gcatgaggat acattgactg aaattaacga gttgaataat tacctgactc gcgacggttt
     1201 tttgctcacc atgacggatg atgagggaaa tatccatgag ctggggacta acacttttgg
     1261 acttatcagt acccaaagtg aagaagaaat tagagaactg gtttcggggc ttacccaaag
     1321 tgcaaccggc aaagatcctg aaatcaccat cacgacctgg gaggaatgga atagcaacag
     1381 aaaataaatg gtttttgggc aataatcagt ctgtggtgtg cgttagctcg tgtttttaca
     1441 ccgcattctt gcgctaacct tatgatctgg cagacaacat gggagagaca tcatgtggca
     1501 ggcaatcagt cgtcttttga gcgagcagtt aggtgaaggc gaaatcgaac tgcgtaatga
     1561 actgcctggc ggagaagtcc atgccgcatg gcatttgcgc tatgcaggac atgacttttt
     1621 cgtcaaatgt gatgaaaggg aactgcttcc cggttttacc gcagaagccg accaactgga
     1681 gttactgtcg cgtagtaaaa ccgtcaccgt gcctaaggtt tgggcagtag gcgctgaccg
     1741 tgactacagt tttctggtga tggattatct cccacctcgt ccgctggatg cgcatagcgc
     1801 atttattctt ggtcagcaaa ttgcgcgttt acatcaatgg agtgaccaac cacaatttgg
     1861 cctcgatttt gataactcgc tctccacaac tccacagccc aacacctggc aacgtcgctg
     1921 gtcaacgttt tttgctgaac aacggattgg ctggcagttg gaactggcag cagagaaagg
     1981 gatcgctttc ggcaatatcg acgccatcgt cgagcatatt cagcagcgtc tcgcctcaca
     2041 tcaaccgcag ccttctctgt tgcacggcga tttatggtcc ggcaactgtg cgctgggtcc
     2101 ggatggcccg tacattttcg acccggcctg ctactggggt gaccgagagt gcgacctggc
     2161 gatgttaccg ctgcatactg aacagccgcc acaaatctat gacggctatc agtcagtatc
     2221 cccgctacct gccgatttcc ttgaacgtca accggtttac caactctaca cgctgttaaa
     2281 tcgtgcaagg ttatttggcg gtcagcattt ggttattgct cagcagtcat tggatagatt
     2341 attagcagca tgatatgggt tgaggataat ggccgctccg tgcggccttt tgattaaata
     2401 aacccaagca gagaaaagaa cacataacct gcagcaatac aaataaccgg caggatatac
     2461 agtgagaaaa actgcaggaa aatagtatgg tgcggaacaa caatacggga ctcaatttgt
     2521 tcacgcgtta gcccctcttc ccctttggct ttttccagaa tgagttgatc ttcaacacct
     2581 tcccgcaaga agcgcgcctg gcgactcatc cgggcaccgg aatcctgcaa cgccagtccg
     2641 acaaaaatca ggatgaaaat cacccagaac ataacgttca cgccaccatt aaaattgggc
     2701 gtcggggagt tataccagaa tagattcaaa aaaggcgtat tcgcctgcat catatcgatc
     2761 atgacatgag taaagtcgag catgaccgca ttaatgcctt cctggttttc ctgccgggta
     2821 ttcataaatt tcagcaaaga aatcaatgta gagatcaatg caggtataaa aatcacccaa
     2881 cccaaaatcc ttttcaaaac agcaatgcgt ccagcttgtt gatacgtcat gagttctcct
     2941 tgattaagac gcgtcgtttc acttagttta cctgtagata tctgttttcg cccattcttt
     3001 aaaggcgata tgataggcgc ttaatcataa gcacggctta ataccttaca cataatgctc
     3061 taaaggagag gttgtaatgt caaccccgcg tcagattctt gctgcaattt ttgatatgga
     3121 tggattactt atcgactcag aacctttatg ggatcgagcc gaactggatg tgatggcaag
     3181 cctgggggtg gatatctccc gtcgtaacga gctgccggac accttaggtt tacgcatcga
     3241 tatggtggtc gatctttggt acgcccggca accgtggaat gggccaagcc gtcaggaagt
     3301 agtagaacgg gttattgccc gtgccatttc actggttgaa gagacacgtc cattattacc
     3361 aggcgtgcgc gaagccgttg cgttatgcaa agaacaaggt ttattggtgg gactggcctc
     3421 cgcgtcacca ctacatatgc tggaaaaagt gttgaccatg tttgacttac gcgacagttt
     3481 cgatgccctc gcctcggccg aaaaactgcc ttacagcaag ccgcatccgc aagtatatct
     3541 cgactgcgca gcaaaactgg gcgttgaccc actgacctgc gtagcgctgg aggattcggt
     3601 aaatggcatg atcgcctcta aagcagcccg catgcgttcc atcgtcgttc ctgcgccaga
     3661 agcgcaaaat gatccacgtt ttgtattagc aaacgtcaaa ctctcatcgc tgacagaact
     3721 caccgcaaaa gaccttctcg gttaatgacc aggggcagtg atcacgctgc ccctggttct
     3781 ttatctgaat ttgcattcaa ctgacggatt aatcgtcaat ttaagagaaa gagttacacc
     3841 gtcaccactt ccgtgcactg tataaaaatc ctatactgta cgtatcgaca gtttagtgag
     3901 ttttatcatg acggcggaag gtcaccttct cttttctatt gcttgtgcgg tatttgccaa
     3961 aaatgccgag ctgacgcccg tgctggcaca gggtgactgg tggcatattg tcccttccgc
     4021 aatcctgacg tgtttgttac cggacatcga tcacccaaag tcgtttcttg ggcagcgatt
     4081 aaagtggata tcaaaaccga tcgcccgcgc ttttgggcat cgtggtttta cccacagtct
     4141 gctggcggta tttgcgctgc tggcaacctt ttaccttaag gttcctgaaa gctggttcat
     4201 tccggctgat gcgctacaag gaatggtgct gggttattta agccacatac ttgccgatat
     4261 gctgacaccc gccggtgttc ccctgctctg gccatgccgc tggcgtttcc gcttgcctat
     4321 cctggttccc caaaagggta accaactgga acgttttatc tgcatggcat tatttgtctg
     4381 gtcggtatgg atgccccatt cattacccga gaacagcgct gttcgttggt catcgcaaat
     4441 gatcaatacc ttgcagatcc agtttcatcg gcttattaag catcaggttg aatactaaaa
     4501 aggcaaaaat cacctttctg gaataagcaa ttccatttga atataagagc cagctcacag
     4561 ttctgttaat cttgcgccaa cactatgact gctacgcagt gatagaaata ataagatcag
     4621 gagaacgggg atgaactttc cattaattgc gaacatcgtg gtgttcgttg tactgctgtt
     4681 tgcgctggct cagacccgcc ataaacagtg gagtctggcg aaaaaagtgc tggtgggtct
     4741 ggtgatgggt gtggtttttg gccttgccct gcataccatt tatggttctg acagccaggt
     4801 acttaaagat tctgtacagt ggtttaacat cgttggtaac ggctatgttc aactgctgca
     4861 aatgatcgtt atgccgttag tcttcgcctc tattctgagc gcggttgccc gtctgcataa
     4921 cgcatctcag ttaggcaaaa tcagttttct gaccatcggt acgcttttgt ttaccacgct
     4981 gattgcggcg ctggtcggtg tgctggtcac caacctgttt ggtttgacgg ctgaaggtct
     5041 ggttcagggt ggtgcagaaa ctgcacgtct gaacgccatc gaaagtaact atgttggtaa
     5101 agtctctgac ctgagcgttc cgcagctggt cttgtccttt atcccgaaaa acccgtttgc
     5161 cgatctgacc ggagccaatc cgacgtcaat tatcagcgtg gtaatttttg ccgcattcct
     5221 cggtgtagct gcgctgaaac tgctgaagga tgatgcgccg aaaggtgaac gcgtcttaac
     5281 cgctatcgat accctgcaaa gctgggtgat gaaactagtt cgcctggtca tgcagttgac
     5341 cccttacggc gttctggcac taatgaccaa agtggttgca ggttctaacc tgcaagacat
     5401 catcaaactg ggaagtttcg ttgtcgcgtc ctacctcggt ctgctgatta tgtttgcagt
     5461 gcatggcatt ctgctgggca ttaatggcgt gagtccgctg aagtacttcc gtaaggtatg
     5521 gcctgtgctg acgtttgcct ttaccagccg ttccagtgct gcgtctatcc cactgaatgt
     5581 ggaagcacaa acgcgtcgtc tgggcgttcc tgaatccatc gccagtttcg ccgcctcttt
     5641 cggtgcgacc attggtcaga acggctgcgc cggtttgtat ccggcaatgc tggcggtgat
     5701 ggttgcgcct acggttggca ttaacccgct ggacccgatg tggattgcga cgctggtcgg
     5761 tattgttacc gttagctccg caggcgttgc cggtgtcggt ggtggtgcaa ctttcgccgc
     5821 actgattgtg ctgcctgcga tgggcctgcc agtaaccctg gtggcgctgt taatctccgt
     5881 tgaaccgctt atcgacatgg gccgtacggc gctaaacgtt agtggctcga tgacagctgg
     5941 cacgctgacc agccagtggc tgaagcaaac cgataaagcc attctggata gcgaagacga
     6001 cgccgaactg gcacaccatt aattcttatg ctggcaacgg tccgttttgt ataggggccg
     6061 ttgccttact ttttaaatgt tccatcgcgg cgatgcatac tgactcatat ttaaacagat
     6121 cgtcagcgaa aaaaatgtcc aacttatcaa tacattcctg gctcaatgaa tgtgcctgta
     6181 gcgagaagct taaaacctca tacccgagac gttttctaag tgaactatct ggaatactca
     6241 taatatcttc aagtatacca cttcccattc ccggaagatt tagtg
//
LOCUS       DAZUCE010000266         6099 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZUCE010000266 DAZUCE010000000
VERSION     DAZUCE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.266"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            105..1160
                     /locus_tag="ACHY03_004519"
     CDS             105..1160
                     /locus_tag="ACHY03_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFS3907672.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETLPPANSQPAAPAP
                     EAKPTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADW
                     LKDFKQGPTGKERTVYGIYGISESSLAECEKGVKSAVALTPALQPIDGVAVSYIDAAV
                     ALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKR
                     QLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQDEFDAEAAMKKVSELETLVAQA
                     KEADKGGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEQLQDANSSWMVEDSFP
                     RALREYNEMVDDYNRLR"
     gene            complement(1262..3010)
                     /locus_tag="ACHY03_004520"
     CDS             complement(1262..3010)
                     /locus_tag="ACHY03_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFS3907673.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQSVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAETI
                     VGLTGYEGFMALLRQG"
     gene            complement(3010..4080)
                     /locus_tag="ACHY03_004521"
     CDS             complement(3010..4080)
                     /locus_tag="ACHY03_004521"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFS3907674.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGTNSREEAEKRGVEIGNFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANNGMISKADYDALLTLI
                     RGFLTTLTEEKVNAFSQFRQVD"
     gene            complement(4070..5521)
                     /locus_tag="ACHY03_004522"
     CDS             complement(4070..5521)
                     /locus_tag="ACHY03_004522"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFS3907675.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5532..5978)
                     /locus_tag="ACHY03_004523"
     CDS             complement(5532..5978)
                     /locus_tag="ACHY03_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFS3907676.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
BASE COUNT         1571 a         1540 c         1546 g         1442 t
ORIGIN      
        1 cgtgaatttt attattttat ttataaacaa aatgcgtgta gtggcgtttt cttttttcat
       61 ttattatgtt cactctctta tctatatatt tataaggcaa ttaaatgaaa aggaatttat
      121 tatcttccgc tattatagtc gccatcatgg ccctcggtct gacgggttgt gatgataaaa
      181 aagccgaaac agaaacgctc ccgcctgcca atagtcaacc tgccgcacca gctcctgaag
      241 cgaaacctac tgaagctccc gttgcaaaag cagaagctaa acctgaaacg cctgcgcaac
      301 cggtggtcga tgaacaagcg gttttcgacg aaaaaatgga tgtctatatc aagtgctaca
      361 acaagttaca gatcccggta cagcgcagtc tggcgcgtta tgctgactgg ctgaaagatt
      421 ttaaacaggg gcctaccggt aaagagcgta ctgtttatgg catctacggc attagtgaat
      481 ccagcctcgc tgagtgtgaa aaaggcgtaa aaagtgctgt ggcgttaacg cctgcgctgc
      541 aacctattga tggcgtagcg gtgagttata tcgatgctgc cgtggcgttg ggtaatacca
      601 ttaacgaaat ggataaatat tacacccagg aaaattataa agacgatgcg tttgcgaaag
      661 ggaaaacgct gcaccagaca ttcttaaaaa atctagaagc ctttgaacct gtagccgaat
      721 cttatcatgc ggcgatccag gaaattaatg ataagcgtca gcttgcggaa ctgaaaaata
      781 ttgaagaaag agaagggaaa acattccact actattctct ggcagtcatg atttcagcga
      841 aacaaattaa taacctgata tcgcaagatg agtttgatgc agaagcggca atgaagaaag
      901 tgtctgaact ggaaacgctg gtggcgcagg ccaaagaagc ggataaaggc ggcatgaatt
      961 tctcgttcat taattcggca gaccagtatc aactcgagac taaaaaatat gttcgccgcg
     1021 tcagagataa agtcccgtac tctgactggg ataaagagca acttcaggac gcaaactcaa
     1081 gctggatggt cgaagactct ttcccgagag cattacgcga gtacaacgaa atggtcgatg
     1141 attataatcg cctgcgttaa acgtttttgt ttttgctgag aaatacaagg tcggatgcgg
     1201 cgtaaacgcc ttatccgacc tacacgtcat taagccgcgt cagctgtgga tccgacatca
     1261 ttcacccctg gcgaagcaac gccataaacc cttcataccc cgttagtccg acaatagtct
     1321 ccgccggatg ctgacacaat acgcttgcca gatagctgaa tatttttagc tcatgacgcg
     1381 catcggcggc ggcgcaggcg atcaaaacat ggttaatgac ttcgttattc acctcaactg
     1441 attgggcgag ggtaataaaa aatccacgaa aacgtcgctc ctgttcgctc cagcaatgtg
     1501 ggatggcgag gcgattaaca atcaggtttt caccttcgcc ttcgcgggcg atgatccgtt
     1561 gcgcttcatc ggcggtaata tggtgttgtg cgactaattg cgcacaaatt tgccgggtaa
     1621 tatgttgcca gctttcaccg cgtacattcg aataatgaaa acttccgggg gcagagaaaa
     1681 aacgctccgg ttgctggcga ataaatgccg tcgccagaaa atgttttatt tgttcgatac
     1741 cggcagccgt aatgatattt tttacggtga tgtaattatt taccgcatca tccagtaaat
     1801 aatggctgtt gttaatgatc aataacggct caatctcttc gcgaatggca acaagttcgc
     1861 ttaagctacg ggcaataatc acccgacaat ttaaaacatc gcgctcaatg gcgagctgat
     1921 taatggtggc aatcgcgttc tggtccgaga gcaaaatgat cggctggcgt tcgttttgat
     1981 gccgttccag cgcacaggca aaatacaaac cgatcagatc gctatcgaaa aggggaatat
     2041 cgagctgttc acgcagtagc gtaataaagt gcagactcat atcaaaggcc gcaggccagg
     2101 cggctttcag gttattcatg ctgctttggc ggtgctcagc aatccacact ggtgcggcaa
     2161 ggcaacgtaa taaatgcccg gtaatgttct caaccagctg cgtatcaata atattcaatg
     2221 acgcgatacc cggcaccctt tgcagcaacc cctgcacatt atcggcagaa attatttgcg
     2281 cttgctgatg ttgtttctct atcaaacccg tcagggttct tacagcgtta tcaccaagaa
     2341 acaggccgct ctgttcaacg atttgtttta tctcgttacc gggatattgc ggccactcat
     2401 cggtcagttg attacgcagg gcgtaaatag ccagaatcag gctggagaga taatcaccct
     2461 gcatgagcgg ccagcggtgt tggttgtcgc aggccgtgga taaatgctga aggttgtctc
     2521 gtgttacgcc cgccagcgga attaaaaacg gatctttgcg tagcaagttc gccagcaaga
     2581 taacgcgttt ctcttctgtc tcatcaatga aatggcccaa accagggcgg ctggccaggc
     2641 aacaagtgcg ttcataacgc tgttttaacc ggggcagacg ctctgcgacc cacgtttccg
     2701 gcaaattaag cgccgaggcg agttgcgcac ggggagtaaa ggtattcagt aataacagtg
     2761 ccagcagccg atcgtcgtta tcgtgctttt gcagcaactg aaaaaaactg cggcgctcga
     2821 agatttccag ctgatagccc gcactgccac tggcgaaaat gcgggctttg ccgttaaggg
     2881 tgaagttgag atagtcaata tcacgcagga tggtcctgcc tgaaacgcca gtctgttgcg
     2941 ccagctcgcc aggcgtgcgc ggctgttgct ccagcagatc gacaatcttt aactggcgtt
     3001 cgtttagcat taatccacct gacggaactg gctaaacgcg ttgactttct cctcagttaa
     3061 ggtcgtcaga aaaccccgta tcagcgtgag cagagcatca taatcggctt ttgaaatcat
     3121 accgttgttg gcgtgcagat aacgagttgg cagacacagc gcgacaaccg gacgcccgcc
     3181 gcccatcacg ttgtagcgcc cgccatccgt cgctccggtt ttcatggtgg aaaattgcag
     3241 cggtaaatca ttctgcgtgg cacagttttt taacgctgct accagtttct ggttggggaa
     3301 gtagcgcttg tcaaacagca tcagccccgg cccctggccc agtttcagcg ggtatttaat
     3361 gttatcaatg cccggaacat cgcccgcgac ggcggtatcc agcacgatca ccacatccgg
     3421 tttagtgtgt tcagccgagg tttgtgcccc gcgtagccca acttcttctt ccacgctgcc
     3481 gacgccgtac agcgtaattt ctgggttatt tactgtctgt agtagctccg ccatcatcgc
     3541 gcagccgatg cggttatcca gcgccttgcc gactacttta tcttcgcccc agcaggcaaa
     3601 attggcctcc gggctaataa aattaccaat ttccacgccg cgtttttccg cttcttcgcg
     3661 gctgttcgtg ccaatatcaa tgaacatctc atcaaatgac agcggctgtt gcttttgctt
     3721 ttcggttaac gcatgaggtg cgacggaacc aatcacacca gggattttaa atcccttgtg
     3781 tgtgcgtatg gttacccggt ggttgagcat cgactgattc caccagccac caatggtggt
     3841 aaagcgcaga aaaccgctct cgtcgatgtg ggtgaccata aagccgactt catccatatg
     3901 cccgacaacg gcaacttttg gacctttatt tcccttacgg gcaacaaagc tgcccagacc
     3961 atcaaaggtg atctcattaa cgcaaggttc cagcgtgttt atcagaatgt cgcgaacttc
     4021 ctgttcatcg ccgctgacgg cgctggcttc gcacaactgt tgcagtaact caatgttcat
     4081 ggacaagctc ctgttgcgcg gctttcgctt tgcggcgtaa ccacaatcct ttcaggacga
     4141 taatgaatga aatgttcagc gcgaggccaa tcgccagcac cagataaaac gaacccaccg
     4201 gcgacatcag gccgatcagc gggtcaaaga tacccaaacc aggtgccaga cgtttgattc
     4261 cgaaagcgat caccagcacg ccggtaatgc cgcccgccag cgtattggcg gtaatcatcg
     4321 gcaacggagc tgccagagcg taaggaatag ccggttcggt tgcgactgtg gcaccgacca
     4381 caatggcact actggcagcc tctttttcct gtcgggtaaa cagtttgggc gcaatgaacg
     4441 tcgcaagacc tgccgcgacg ggcggcatca gcgcgacaac gccgacgatg gcgtaccagt
     4501 cataaatgtg tttttccagc agtgagaagc agaagaacca ggcggtttta ttgatggggc
     4561 cgcccatatc gaacgccagc atcgcaccga ccagaaacgc tgccgcaaac ttcattgatg
     4621 gaggaatggt attgaggaag tgcagtagcc cgcccatgag gtcggacatc accggtccga
     4681 taacgtagta agtgagcacg ccgaaaacca gcagagtgac gaacgggatc agcattgaac
     4741 cgagcagcgg ttgtaatgct ttgccaagac ggactttacg gaaccagaag acgaaatagc
     4801 caatcgccag ccccagcacc accgcgccga ggaatcccgc acccgactgg gtgccgagca
     4861 gcgctttgtc gttcgccaga tagcaaacca gaaacgccgg agcgaaagcc ggtttatcgg
     4921 caatcgacga ggcaatataa gcgcccatga tggggatcat aaaggtgaag ccaaggtaac
     4981 cgatggattc aactacccag gtaaacgacg gcgcgccttt cgacatatcg gtatacggca
     5041 aaccgaactg caccagcatg ttggcaagcg ccaccaggat accaccgccg atgacaaacg
     5101 gcagcgccgc agagacgccc gccatcaggt ggctcatcac gctgccgctt tgtacttcct
     5161 gtttgcctag cttcacgccg ctatctgcgg caaaaagctg cgagtttgtt ggtaattcgc
     5221 tgaaaatctg atcgatattt ttcagcgcct gggagatggc aatctcataa acttttttcc
     5281 cggcaaaccg tgcgcgatcg tcaccgctca ggccacgccc ggtggcgaga atcacgtaat
     5341 cggcagcggc aatctcttcg ctggataagc ggttttcaac gccactggac ccttgagttt
     5401 ccacttttat ggtatggccg agagaacggg ctttctgttc cagggcttcc gccaccatgt
     5461 aggtgtgagc gatcccggcg gggcagttgg tgatcgcgac aatacgtaag gatgactcca
     5521 tcgccacttc cttatgagct gagggtttga tttaacaagg caagcacctg gtcggcgtcg
     5581 ccctgttgca gttggtgaat aaaatcctgg tgaataattt tgcgacacag tgtgccgatg
     5641 attttgacct gatcctcttc gccactttgc ggcacgccga ggcagatcca gcaattgacg
     5701 tcttcgccgt cgctggcttt ccagtcaata gcctgcgctt tgcgggcgaa taacacgaac
     5761 ggttgtttaa cgcaggcgct tttaccgtgc ggcacggcga caccagaacc aaaaccggtg
     5821 gagtgcattt tttcgcgcag cagcagggtt tgcaggaact ggtgtgagtc ggtgataaaa
     5881 ccgttttgta acgccattgt cgccaactgc ttcagaacgg aataagcgcc attgccctga
     5941 atattcaggt caatacagct tgcagtaaga gctgccatta atcggactcc atgaaaaaat
     6001 atttcagtta attgtaatag cggggcgggt ttgtgatgag cgcggatatg tcgtgaagtc
     6061 gtgacaatat aaagcagagt gaaggataaa ttttgcggc
//
LOCUS       DAZUCE010000267         6076 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZUCE010000267 DAZUCE010000000
VERSION     DAZUCE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.267"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..431
                     /locus_tag="ACHY03_004524"
     CDS             <1..431
                     /locus_tag="ACHY03_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFS3907677.1"
                     /translation="DISGRWGFDGEKITDLLTAEKARGMKGDEINAWRNAMEAANYTF
                     EHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFWTDAENNDVEVTAEELIALSEAAE
                     QAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRVGWAQE"
     gene            969..2594
                     /locus_tag="ACHY03_004525"
     CDS             969..2594
                     /locus_tag="ACHY03_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907678.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            2591..3859
                     /locus_tag="ACHY03_004526"
     CDS             2591..3859
                     /locus_tag="ACHY03_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS3907679.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            3874..4152
                     /locus_tag="ACHY03_004527"
     CDS             3874..4152
                     /locus_tag="ACHY03_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907680.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            4158..4775
                     /locus_tag="ACHY03_004528"
     CDS             4158..4775
                     /locus_tag="ACHY03_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907681.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            4866..5600
                     /locus_tag="ACHY03_004529"
     CDS             4866..5600
                     /locus_tag="ACHY03_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS3907682.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            5832..5972
                     /locus_tag="ACHY03_004530"
     CDS             5832..5972
                     /locus_tag="ACHY03_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3907683.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
BASE COUNT         1596 a         1249 c         1691 g         1540 t
ORIGIN      
        1 ctgacatctc ggggcgctgg ggttttgacg gggagaaaat cacggacctg ctgaccgcgg
       61 agaaagcgcg cgggatgaag ggcgatgaaa ttaacgcctg gcgtaatgcg atggaagcgg
      121 cgaactacac gtttgagcac aatgggcgta aatgggacta cgggaagtca acgcagacgc
      181 gtcttgagcc gtcggtggct gcagcgaaag cggggaaact gccggaggcg tttttctgga
      241 cggatgcgga aaacaatgat gtggaagtga cagcagaaga gcttatagcg ctgagtgaag
      301 cggcagagca ggcgatgttc accaaaggga tggaaatcca cgtccggcag cgcaccatga
      361 agaaagagct ggaaaagctg accagtgcgg atgagatact ggcatacagg gttggctggg
      421 cacaggaata acagacaaaa agatggggga ccatcacctc ccccatgaac tgagtcaatt
      481 atacacttgt gaagtattta ctatgcgaat aaatactgtc acggaaagtg caggcctgga
      541 tgtgatggaa atcatgtttc cggcgcatcc tgttgatatg gataattttt cagatttccg
      601 gaagtgagag tttttggtgt ttccatacca gccacagttg atgtgtgttg tggcgcttgg
      661 cagtgcgatt aaaggagcag agggaataaa aaacgggggc atgaccaacc cccggtacaa
      721 ggaaggagta actacaatgg aaaaattaat gaagaccttt agtgctacgt tatatggcga
      781 ataaagactg ccacagggaa gtttgcagaa acagtgatgc cgatcgtgtt tctgacgctc
      841 ttctctgata agagtaattt ttcagatttc tggaaatctg tattctgcac aggcgcaatt
      901 gtatgccgcc tttaaaactt caattcagtg actcacctgc cattcaaatt ttcggatacc
      961 agacaaccat gccttatatc gatataacaa ctatgcgcgg gatgatgcca ggcgttattg
     1021 catctatgct gccagatcat tctgctgtac tggcagaaaa ctgtcatttt cgctatggag
     1081 tgatcacgcc tgaacaccag atgtcagagg ctgagaaaac attcgcgatt aagccgaaaa
     1141 ccatttttca ttaccgtgac gatttctggt ttgcatggac ggatgtggtg gatgtgatcc
     1201 gcagtccggt cgctcaggac tcccacgggc gtatttacta cactgacggg cgttttccta
     1261 aagtgacgga tgcgaccatt gccacaaaag gggacgggaa tcacccgaca tcatcgtatc
     1321 gtctggggat ccccgcgccg acgacagcac ctgtctgtac tgttcagcag ggcggtgatg
     1381 tttctgacga taacccgaat gatgacgaaa cccggtttta tacggaaacc tttgtctcag
     1441 attatggtga agaaggtccg ccaggtccgg cgtctctgga ggtaacactc cgtactccgg
     1501 ggactgcggt acagctgacg ctgtctccgg tgccattgca gaatgccagt attaaacgcc
     1561 gccggattta tcgctctgca tcaggtggag gagaagcgga ttttttactt gtggctgaac
     1621 tggatgcatc cgtgctcagt tacacggaca aaataccggg gaaaaacctt ggaccttctc
     1681 tggcgacatg ggattacctg ccgccgccag agaatatgac aggcctttgc ctgatggcta
     1741 atggtattgc cgccgggttt gccggtaatg aagtgatgtt ttcggaagcg tatctgccgt
     1801 atgcatggcc ggaagtgaat cgtcacacga cggcagaaga tattgtggct atctgtccgc
     1861 tgggaacgtc actggtggtg gcgacaaagg gggagcccta tctgttcagt ggggtatcgc
     1921 cttccacaat ttctggctcc agaattcctt ccatgcaggc atgcctgagc cgaagaagta
     1981 tggtggcgat ggagggattc gtactctatg ccgggacaaa cggtctggta tctgttgatg
     2041 taaacggtaa tacagcactg gcaacggaaa agattatttc acctgaacag tggcagagtc
     2101 agtttaaccc ggcgtccatt gtggcttatt cctggcgtgg tgagtacatt gcctgttaca
     2161 cgaaaccgga tggtaagcag gatgtgtttg tattcagtcc ggtgaacatg gatatccgtt
     2221 atctcagtac accgtttgac tgcgcatggg ttgatctcgc gaaagatatg atgcgcgtgg
     2281 tgacaggaga caaaatgtca gtgcttgccg ggggctcgct gccctccacg ataaggtggc
     2341 attcaaaaat tttttcatta cctgaaagaa cctctttttc ctgtatcagg gtgaaatctc
     2401 cggcgcctga gcgggtgggg atcaccatta tggctgatga tgttcctgtg attcattttg
     2461 cgccgggtac gtttaaggga agtgtggtga gacttccggc agcaaccggg caaaactggc
     2521 aggtgatggt atccggattc gggcaggtgg aacgaataac cctgagtaca tcgatgtcgg
     2581 agatgccggt atgaccagaa aaccgtggcg tgcggggaag gatttatcca cagttgtgga
     2641 gaacatggaa attggcaccg ggcagcgtgg tgacggacgc cacgcatttg tgacccgtga
     2701 ggaactggtt ggtcttaaac tcgcccggcg tcgaacatcg ggtggtgcct catatgcact
     2761 gaatccgggt attgagattg acagtacttt aatgactgtt gattttccca caaaaccgct
     2821 gaattttaag gcgacaggag gatttggctc ggttcttctt gaatgggata tgcctaatta
     2881 tcgcggacat tcactgactg aaatctggcg gggtacggag gatgaccttg ctgatgcagt
     2941 gctggttgcc acgacgccgg ggcaggttta cggcgatccg gttgaccctg gctggtcggg
     3001 attttactgg atacgttttg ttaacgcggc aggagtgaaa ggtccatgga atgctgaaaa
     3061 aggcactcag gcacaaacac agatcggcgt gaaggccatc attgaccaga tccgcgatga
     3121 ggctgcaaag tcgccggttg tgtccgagct gcgtaaagaa ataaaaaacg cgcaggggca
     3181 ggctgtaaag gatgctgcaa ttaagacaac cgaagttgtg gggactctca gggaagaaac
     3241 gacaagaacg attggtggta ttgaaacccg cattagcaca ctggattcat caaccagtga
     3301 atcgcttaat gaggtcgaca agcgcatcac taaactggat aaagaaggcg gtgaggcgtt
     3361 tctggcaatg tggtcaaaaa aagcgggagt tgatggtatc actgcgggga tcgggattgt
     3421 cgccggaaaa gacagtgaag gcaggcctgt aagtcaggtt gcaatttctg cgtcgcagtt
     3481 gtttgtcttt gacccgaaca acccggataa caccgcctat ccgtttgcgg tatcaggtgg
     3541 caaggttgtg atcccgaaag cgatgattta tgacgcggtg attgaaacac tggtgtcgcg
     3601 gaaggttgtg gcggatgagg taaaagccgg ggtaagtatc acttcgccag ttatccggag
     3661 tgccgttatt cagaacggaa actttcaggt tgattctcag ggtaacctga atattggagg
     3721 ccttttcagt gttacgtcac aagggcaact gacaattcgt tactctaatc agaatgtagg
     3781 actggtgatc cgcaatgata aaattgaggt ttatgatcag aatggacgac tggctgttcg
     3841 cataggcaga ttacgctgat caggaggtga gtattggaat acggttttgc catttataac
     3901 agaaataacg ttaatgttac gggcgtgctg actccggtat ttttcctgga cagatttaca
     3961 gcggagtctg gctcaaagac gtacacgaat aaacccgacg ggaaatcatt gcaggctgta
     4021 tgttgtttat ttccctggaa taacgtattt gcggatcgga aagtaccgaa gataaccatt
     4081 aatggcaata cggtgacgtg gtcgaatctt gagcagggta tgggatctta tatttataca
     4141 ttctggggat aagtgtcatg tatggtttga gcattatgaa gccggatggc agcgtatgga
     4201 taagtccagg ttttacgccg cagtgtctga tcaacaaagg caccataccg gcgactgaaa
     4261 agtctttttt taaaacatca atcccgtcag gcaaaagttg ttttttcttt atcagaacag
     4321 agaagaaggc cgatgtcatg tacacgcatg aacagattga tggatatcat gcactaaggc
     4381 ttcatgtaat tgtcagggga acgaaccctg gtgttacgac ggtttatgct ttcgcgaata
     4441 tggttactcc accttctgag tatggtatcg ccatgtataa cccggacggt gagatgattt
     4501 atcatggcga aatgatgctg cttgacgcga agttaatacc tgttgatatc aaatttgaaa
     4561 aggaccttgg atatccatgc gcaatcatgc ctgcactggt cgggtattat aactggaaaa
     4621 gaactcctta tgatcgaccg atttatacca catccactgg tgctacagga aataaaatat
     4681 attcctgtga gcattattcc ggtggtgcaa catgggatat tcgaaagccg tatatagata
     4741 aggtcctggt tattaataca tcagtatatg attagttgaa gcgagtcttt aatattcatt
     4801 taaaatgtct aaaaagatgt attattaaaa agtttagcgt gttatctgaa tacaggatat
     4861 cttaaatgaa gagtatagca acactggttg tgtgtgcaat ctccgggatt gcctgtgtaa
     4921 atttatctgc acatgcagca gaaggaaacc atacaatttc tctggggtat gcgcactttc
     4981 agtttccggg actgaaggat tttgtaaagg atgcgactgc tcataacagg gagactttca
     5041 gtcatttcgt caacagaaac tacttttctt cattgggcga atatacagat ggtcgggtca
     5101 gtggatatga aggcaaggat aaaaatccac agggcattaa tatcaggtat cgctacgaga
     5161 taacggatga ttttggcgtt atcacctctt ttacatggac gcgttctctc actaactcac
     5221 agacatttat tgatgtgcag tcagccgatc ataccaggaa gattaagaat ccggcagctt
     5281 ctgccagaac ggatatcagg gcgaattact ggagtctgtt agcggggcct tcatggcggg
     5341 ttaatcagta catgagttta tatgcgatgg cagggatggg cgttgctaaa gttagcgctg
     5401 acctgaaaat taaggacaat attaacagta gtggcggatt ttctgaaagc aacagcacga
     5461 aaaaaacctc ccttgcgtgg gctgcaggtg cacagtttaa cctgaatgag agtgttacac
     5521 tggatgtggc ttacgaaggt tccggctctg gcgactggcg cacgagtggc gttactgctg
     5581 gcattggcct gaaattctga cctgtatccg gtaaccgttt actacccgct gtgatggcgg
     5641 gttttttatt gcccgtacag ggcaaaaacc gtaaattatg cgtgggtgcc tttcggctga
     5701 tggctggagg gagaacctga aggcctgatg tggaaaggcc ccgagtcaac ttaacgttaa
     5761 cccgaggccc taatcatcat accttaagca agtagaaggt tagcgcctct ctgtaaaagg
     5821 agtcaagcgc tatgtcgcaa aaatcgctta tcaccgtcac aatttgtatg acggttatct
     5881 tcactatctg gatgttgcac ggttcgctgt gtgagttccg gctgaatttg tggggagcgg
     5941 agtttgcggc gttcttacag tgtaagcagt aggaaaaccg cgacggggac gaaagtcccc
     6001 gtcaactggt tgctgaggtt cagccgatat ggcacccgtt tgtagtgtat gttttataca
     6061 atgcaggctg acagag
//
LOCUS       DAZUCE010000268         6036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZUCE010000268 DAZUCE010000000
VERSION     DAZUCE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.269"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..560)
                     /locus_tag="ACHY03_004531"
     CDS             complement(<1..560)
                     /locus_tag="ACHY03_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS3907684.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIADATGK"
     gene            complement(711..908)
                     /locus_tag="ACHY03_004532"
     CDS             complement(711..908)
                     /locus_tag="ACHY03_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFS3907685.1"
                     /translation="MLKQQDMTETARVVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(1082..1795)
                     /locus_tag="ACHY03_004533"
     CDS             complement(1082..1795)
                     /locus_tag="ACHY03_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907686.1"
                     /translation="MDDSALLRNSSLFVAYMGCLGWGSAYFYGWGTSFYYGFPWWVVG
                     AGVDDVARSLFYAVTVIVIFLIGWGVGIVFFLGIKQKRNIQNLSFIRLFLAILLLFIP
                     PVLEFSVIHQHVEPDVLIFCVLAAFAITLFVRSGRRLVSVKCFSEMSVIRHHRIEFMM
                     AGFMIYFWAFSLIAGWYKPQFKREYQAVHYENVWYYIIARYDDRLVLSKSYRSGGKKF
                     VIFNSGNINDFEINTVRVR"
     gene            complement(2049..2714)
                     /locus_tag="ACHY03_004534"
     CDS             complement(2049..2714)
                     /locus_tag="ACHY03_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFS3907687.1"
                     /translation="MNNQYLQFVREQLMIATADLSGSTKGQLEVWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEHQIAITQWAWTEFNAQSGTRKIAGKTLVRLKTLIWLAAQAVKAE
                     LFGGEGYEYQELALLVGVTTKNWSKTFTGHWVAMKHIFHRLDSEALLLVEGTRSKQKA
                     AFS"
     gene            complement(2707..3069)
                     /locus_tag="ACHY03_004535"
     CDS             complement(2707..3069)
                     /locus_tag="ACHY03_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFS3907688.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLRLSGRLAIKIIAEPPDKRRRDLDNFLKAPLDALTHAGMLMDDEQFDEINIVRGK
                     PVSGGRLEIRITEVGVHE"
     gene            complement(3082..4131)
                     /locus_tag="ACHY03_004536"
     CDS             complement(3082..4131)
                     /locus_tag="ACHY03_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS3907689.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESRPVFHCGRVLVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARCSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPQEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            complement(4133..4405)
                     /locus_tag="ACHY03_004537"
     CDS             complement(4133..4405)
                     /locus_tag="ACHY03_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907690.1"
                     /translation="MAQRIKSHNSEAVPAIKRGRHSHVFKTDWYQHPPCTEEQAEWLI
                     QCYRRRGYEVKKAISLDYRHWIISVRLPYSEHPPRPSRTFQQRIWR"
     gene            complement(4572..4727)
                     /locus_tag="ACHY03_004538"
     CDS             complement(4572..4727)
                     /locus_tag="ACHY03_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3907691.1"
                     /translation="MKQQKAMLIALIVICITVVVTVLVTRKDLCEVRIRTGQTEVAVF
                     TAYESEK"
     gene            complement(5018..5533)
                     /locus_tag="ACHY03_004539"
     CDS             complement(5018..5533)
                     /locus_tag="ACHY03_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907692.1"
                     /translation="MSEINYQEGHETAGQAKPVAWRYRYVKKDVTDFQGKPWAGDWKY
                     VPTKEDCNDRPNYEIQALFIGPPVPVTSEGLVKAVRFYEQVKRENPPVETGAWKDAVD
                     WVLREACCAAILGKADNPPASGNQVSELAMWVKRLVSQLKKAKPDCKLPDKAMDYLKR
                     NGLINEEDILR"
     gene            complement(5530..5706)
                     /locus_tag="ACHY03_004540"
     CDS             complement(5530..5706)
                     /locus_tag="ACHY03_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907693.1"
                     /translation="MADFGSTKYNVSFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            complement(5699..5881)
                     /locus_tag="ACHY03_004541"
     CDS             complement(5699..5881)
                     /locus_tag="ACHY03_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907694.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
BASE COUNT         1516 a         1632 c         1298 g         1590 t
ORIGIN      
        1 ttctttcctg tggcatcagc aatctgtttt gccgttatcc ccagggcagc acgtgcatca
       61 cgaaagtaag caatcagcgg ggccatcaca tgctgtttca gtgccctgcc cttcgcctca
      121 tacccggcat ctttcggacg atacggcccc tgataatgtt ccgcgaacag aatgcgctct
      181 gtggcgggga aatacgccct caggctttcc ttgttgcacc cgttccagcg tccggacggc
      241 ttcgcccaga taatatggtt cagcacactg aagcgttcac gcatcatgat ttcaatgtca
      301 gatgccaggc gatggccaca gaacaggtaa agacttccgg caggtttcag cacccgccag
      361 aactgcgcca gacactggtc cagccacttc aggtaatcat cgtcgccctt ccactggtta
      421 tcccagccct caggcttcac tttaaagtac ggcgggtccg tgactatcag gtcaacagaa
      481 ttttcgggta acgaccggat aaattccagg cagtcggcgt tgattaactc acaactggat
      541 atttttacag tattaagcat ggatcattaa gcctgtctct gataggctca ttctgctttt
      601 gcgcaaagca gtgggcctga ggtttgcttg tgatccggac gcatgagcag atggctggtg
      661 agtgccccta acacccacca gccgcccatt taccacaaat aaaaaagcct tcaggactga
      721 aggcgtctgt aacaaccgaa ctgatagtct gccagacccg ccataacaag ctgggtcagt
      781 attaactggc agcgttcacg tgaaagataa gtattctgcg caatctcccc gactgtcgcc
      841 ggttcggtgg cacttaattc attaaacacc actctggcgg tttctgtcat atcctgctgt
      901 ttcagcatgt ctttttccct tttccggtta acgtgacaca ccaataactc ttgtcgaaaa
      961 agccagcaag ctgaaagacc ggtattaata accacctgca cattttatgt actgcaccat
     1021 ttttctggca taaaaaaacc gctcaatgcc gggcagtaaa atctttatta ctcaggaaat
     1081 tttaacgtac tctgacagta ttaatttcaa aatcattaat atttccgcta ttaaatataa
     1141 cgaatttctt acccccactc ctgtatgatt tcgataacac cagacgatca tcatagcgcg
     1201 caataatgta ataccataca ttctcatagt ggaccgcctg atattccctc ttaaactgtg
     1261 gtttgtacca accggcaata agagagaatg cccagaaata aatcataaac ccagccatca
     1321 tgaactcaat tcggtgatgg cgaataacag acatttccga aaaacatttg actgaaacaa
     1381 gtcttcttcc agacctgaca aaaagcgtga ttgcaaaggc agcaagaacg cagaaaatca
     1441 gtacatctgg ctcaacatgc tgatgaatta cagaaaactc cagaacaggt ggaataaaaa
     1501 gcagcaatat cgcgagaaaa agccggataa aactcaaatt ttgtatattg cgcttttgtt
     1561 ttatgcccaa aaagaaaaca ataccaactc cccatccaat aaggaatata acaataactg
     1621 tcacagcata aaacaaactt cgtgctacat catcgacacc agccccgaca acccaccatg
     1681 gaaagccata gtaaaatgaa gtaccccatc catagaaata agcgctcccc catccgagac
     1741 agcccatata agcgacaaaa agtgaagagt ttctgagcag agcactgtca tccatagtaa
     1801 caccattaac aactcaaaaa tatcaacaca cattacataa caaattggat tccatgcagt
     1861 caaggggcgt cattgatgga gaaagtattg gcacaatcgt catcacgttt aatgtctatg
     1921 ccattttttg gggggtaaaa aacccgctcg gtcacgggtt ttagtggctt tgccatcacg
     1981 tataataacg gcaaaatatc agatttacac gaaatatatg cctttttgtc tacttttgca
     2041 atactttgct atgaaaatgc cgccttttgt tttgaacgag ttccctccac caacaataaa
     2101 gcttcactat ccagccgatg aaaaatgtgt ttcattgcaa cccagtgacc agtaaatgtc
     2161 ttggaccagt ttttggttgt tactcccacc agtaacgcca gttcctggta ttcgtaacct
     2221 tccccaccaa aaagctcagc ttttaccgcc tgcgccgcca accagatcaa cgtcttcagg
     2281 cgcaccagag tttttcctgc aatttttctg gtaccggatt gagcattaaa ttcagtccac
     2341 gcccactgcg ttatcgcgat ctgatgctcc cagcaaatgc taccgctgta acaccacagc
     2401 agccaggctt tatgatgttc ttcaagagac agaacggcgc gtcgccatga tgatgtcgaa
     2461 aactcaaccg gactgaccag ggcaattgat gagcctttcg ccagtgattg tttacccggg
     2521 atcgggggat tatcccgcgt gatcattttt ccggttacct catcgcggta acgaattttt
     2581 ttgcgtctgt aacgccctgt atcgaacagg gcattttcct gccagacttc cagctggcct
     2641 tttgtcgacc cactaagatc cgcagtggca atcatgagtt gctcacgaac aaactgtaaa
     2701 tactggttat tcatgcacac ccacctctgt aattcttatc tccagccgtc caccagatac
     2761 tggcttgcca cgtacaatat tgatttcatc aaactgctca tcgtccatta acattcctgc
     2821 atgcgtcagc gcatccagcg gcgctttcag aaaattgtcc aggtcacgac gacgcttatc
     2881 cggtggctct gcaataattt ttattgccag ccgtccggac agtcttaatt tcagccgctg
     2941 ctggcgaaca ataagcgcca ctgcccggcg ataacgctcc ccggcttttg atacaaaata
     3001 tgtgctgcca cggcgtcgcc agtaagtgtt caccgtcggc gggtaaggta aaaccaaatc
     3061 tatgagcatc agtcacctct tttacccgag cacgccagtc gcaaaggcgt gatcaagaaa
     3121 acgaaaaatt aactcaatct gagagccgta ctttttctca aactcctgcg ggtctgcatg
     3181 aagttcgttg tggtgctccc ggcacaacgg tagcgtgaaa atatcgtggg cctttgttcc
     3241 cattcccccc tgaccatgac caatcaggtg atgcggatcg tcagctggct taccacaaca
     3301 cgcacacggc tgtgtcttta cccagcgcgt gtatttctca ttaacccaac ggcgacgttt
     3361 aggccgcctc atgaacgatt caggagactc cggatcaacg gcgatactga caaccgtttt
     3421 tttctgtggt ggattttgtt gctggtggac atgaagtggc agcgcaatat tttttgtgcg
     3481 ctgcttcagt atgctgatgg ctgtctgttc tcccggtacg atgtcactct cacggtatac
     3541 ggagcggatt ttttccgctg gtaatcccag cgaacaacgc gctactgcct caggtagtgc
     3601 atccaccacc tgattgcagg ccgcccacca ggataattcg gccagcgata actccctctc
     3661 ctgcgtaccg cttattgcgt gacggatgac gtcaatcatc caggcaacca gattctgctg
     3721 agcaagttga tcgagtgatt ctgatgtctg gtcgcgcagc tggttgtcac agtgccagca
     3781 caacaccatc gcgccggtac cataacggtg aatgacggtt tcgctgtgat gataatcgcc
     3841 gtgtggccac tggcaggatt tcacgtgacg taataaccag tcagacagtg cacctgcacc
     3901 acctgctgca cgaataaccc gctcatcgct gaaaaatggc agtaatgttt tatcctctgc
     3961 cagcggctgg cgaacggcag gaacgactcc ggacggcaga ccgcgcatgt ttttcggttc
     4021 cggctccacc agcaccctgc cgcaatgaaa aacaggccgt gattcgcgtc caggcttaag
     4081 gaccaccagg ccaagttccg gcaccagaac aggtcgaagt aatacccgca cgttacctcc
     4141 agatacgttg ctggaatgtg cgggacggac gcggtggatg ttcggaataa gggagcctga
     4201 cagagattat ccagtgacga taatcgaggc tgatggcttt cttaacctcg tatccgcgcc
     4261 tgcggtaaca ctgaatcagc cattcggcct gttcttcagt gcatggggga tgctggtacc
     4321 agtcggtttt aaatacgtgc gaatgccgcc cccgcttaat ggccgggacg gcttcagaat
     4381 tgtgggattt tatacgttgc gccatcgggt tctccggtga cacagcaggt gccagttgtt
     4441 caggccggcg tgcgaattgt aaaccagaat gccaggaaaa aacaaaaccc gcagaagcgg
     4501 gttaagtgcg ggtgcgttga ggatgcctga cacatcagag gtggcgaggg atttctccct
     4561 cgccgggtct cttacttctc agattcgtaa gctgtgaaga cagcgacctc cgtctggccg
     4621 gttcggattc gtacctcgca gaggtctttc ctcgttacca gtaccgttac aacgacggta
     4681 atacagatga cgatcagggc gattagcatc gccttttgct gcttcatagc ctgctgctcc
     4741 ttgcctttcg gcgcataaga ggctaatcta agtgtgcaag tcatagatat ggcctcagat
     4801 taatgttaag cgtcctgcaa gacgcataat gttaactggg gcttttctct gtctgcctta
     4861 cgtcggcatg cccgaggcag acagcctcaa gcacccgcag caattctact taactcttct
     4921 ttccccgcaa accgttttta tccccagcgg caaatcgaat acaccaccag cgccaccgcc
     4981 attgcaattc ctaccgttat gaacgcttca ggccaggtca tcgtaaaata tcctcctcgt
     5041 ttatcagtcc gtttcgcttc aggtagtcca tcgccttatc cggtaatttg cagtccggct
     5101 tcgctttttt cagttggctg accagccgtt taacccacat tgctaattcg ctaacctgat
     5161 tgccggatgc tggtggattg tcggctttac ccagaatggc agcacagcag gcctctctga
     5221 gcacccagtc aacagcatcc ttccatgctc ctgtttcgac tggcggattc tcacgcttta
     5281 cctgttcata aaagcgcaca gctttaacca gtccttctga tgtcaccggg actggcgggc
     5341 cgatgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct tttgtcggta
     5401 catatttcca gtcaccagcc cacggcttcc cctgaaagtc tgtaacgtct tttttcacgt
     5461 agcgatatcg ccatgcaact ggttttgcct gccctgccgt ttcatgccct tcctgataat
     5521 taatctcgct cattcatcgc cccactcatc acaatatgct tcgaccggag tttttcctgc
     5581 ttcataatca tcacgccatg cttcagcatc agcagcactg ccaccacgta actctgcata
     5641 gtccattaac agttcatgcc attcttcaaa actgacgttg tatttagttg aaccaaaatc
     5701 agccattttg ttcttcctct tcgtctttta tttcgtgata tgagtaattg cagtagttaa
     5761 agaaaatttc ttttgcttcg tcatgaattt catcaggtgt tgcgtcatcg tccacttcga
     5821 atacatcctc aaaatctcca ccggctattc ccgtttcaat aattattttg aactttcgca
     5881 tttcactacc gccctgccgg gcggtctcct gatgttctga gggtgcagaa atccctccgg
     5941 ttaaggatta aattttattt acagtgctga ttttaattat tcagttctgg attatgcttt
     6001 ctctttcacc tgccgtagtt cctggtaatt aatttc
//
LOCUS       DAZUCE010000269         5985 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZUCE010000269 DAZUCE010000000
VERSION     DAZUCE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.270"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            170..898
                     /gene="gadW"
                     /locus_tag="ACHY03_004542"
     CDS             170..898
                     /gene="gadW"
                     /locus_tag="ACHY03_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFS3907695.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1261..4374)
                     /gene="mdtF"
                     /locus_tag="ACHY03_004543"
     CDS             complement(1261..4374)
                     /gene="mdtF"
                     /locus_tag="ACHY03_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFS3907696.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLHCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4399..5508)
                     /gene="mdtE"
                     /locus_tag="ACHY03_004544"
     CDS             complement(4399..5508)
                     /gene="mdtE"
                     /locus_tag="ACHY03_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFS3907697.1"
                     /translation="MLTACDDKSAENTAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQI
                     QGSTPVQLNLENGKRYNQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5616..5894
                     /locus_tag="ACHY03_004545"
     CDS             5616..5894
                     /locus_tag="ACHY03_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907698.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5895..>5985)
                     /locus_tag="ACHY03_004546"
     CDS             complement(5895..>5985)
                     /locus_tag="ACHY03_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFS3907699.1"
                     /translation="LKIQKFNIMYKLKLRRMSDIVTLGITSYF"
BASE COUNT         1469 a         1492 c         1501 g         1523 t
ORIGIN      
        1 tattcgtgtt acatttactt attatcaatt aactgttatg caaaactact ttgtggataa
       61 attttggtcc taccaaatct ggcagttttt gcgctaagaa acagtctggc atcatttcat
      121 tagtatactg aaattgaaat aatcgcagta tgaaatataa gggataatca tgactcatgt
      181 ctgctcggtg atcctcattc gtcgttcatt cgatatttat catgaacagc aaaaaatatc
      241 gctgcataac gagagtatcc tgctgctgga gaaaaatttg gctgacgatt ttgcgttttg
      301 ttcaccagat acgcgacggc tggatatcga tgagctgaca gtttgccatt acttacaaaa
      361 tattcgtcag ctaccacgca atttagggtt acatagcaaa gaccgtctgt taattaacca
      421 gtcacccccc atgccgctgg tgacggcgat ttttgatagt ttcaatgact ccggggtaaa
      481 ttcgccgata ctgagcaaaa tgctctatct ttcctgttta tcgatgtttt ctcataagaa
      541 agaactgatc cccttacttt tcaatagtat cagtactgtt tcaggaaaag ttgaacgcct
      601 tattagcttt gatatcgcca aacgttggta tctgcgcgat attgcggaaa gaatgtatac
      661 cagcgagagt ctaatcaaaa aaaagttgca ggatgaaaat acctgtttca gtaaaatatt
      721 actcgcctcc aggatgtcga tggccagacg attactcgag ttacgtcaaa ttcctctgca
      781 tactattgcg gaaaaatgtg gctatagcag tacgtcctac tttataaaca catttcgaca
      841 atattatggt gtaacgccac atcagtttgc gcaacattcg ccaggtacct tttcctgaca
      901 tatttcgcat ttgaatattg gtcaggatct cacacctgct tcatgtgaaa ctcttccctg
      961 atgatttctg ccgggctacc ggctagttct ctttcgcagt ttgccaggcg gtataggtat
     1021 tttcaggagg aaaagactcg gcatgtttgg gattattaag ctgacaattc atacgattac
     1081 gggaatgtgg gtcagtattg tgttgttcaa attgatgacg aatggctgga gtggtttcta
     1141 ctttcagtgc tgtgtactaa gcctggtatt cctgacggtt agctggttgt tgagtggtga
     1201 atggttagca ggaaagagta aggctgaacc ctcacgttca accttactct catttacacg
     1261 ttacgctttt ttaaaacggg caaagagatg ttccactaca acgaaaaaga ccggaacgaa
     1321 gtaaattgcc agcactgttg cggcaaacat cccgcccatc acgccggtac ctaccgcgtt
     1381 ttgcgcgcca gaaccggcac catgactgat aaccagcggc agcacgccga gaataaaggc
     1441 cagagaggtc atcaggattg ggcgtaaacg cattcgtgcc gcttcgatga ttgcctctat
     1501 cggcgttttc ccttctttct gcatcatctc aacggcaaat tcgacaatca ggatggcgtt
     1561 cttggcggaa agcccgatgg tggtcagcaa accaacctgg aagtagacgt cattacttaa
     1621 gccgcgcaga tcggtggcca gtaatgcgcc aacgacgcct aacggaacaa ccaacatcac
     1681 cgagaacgga attgaccagc tctcataaag tgcggcgagg gcgaggaaca ccacgaccag
     1741 tgaaatcgca tacagcgcag gagcctgatt tgaggataac gcttcctgat acgacagtcc
     1801 ggtccatgag tagccgacgc ctgccggaag tttagcgacc aggtctgcca taaatttcat
     1861 tgcgtcaccg gtacttttcc cggccgccgc ttcacctaaa atctccattg acgggatgcc
     1921 gttgtagcgt tccagtcgcg gtgaaccata ggtccattcg gtagacgagt aggcagaaag
     1981 cggtgccatc gtgccagagg cgttgcgtac ataccattga ttgatgttat ccggcaacat
     2041 acggaacggc gtgcctgcct ggacatacac ttttttcacc cgcccctggt tgaggaagtc
     2101 gttcacgtag ctgctgccga aggcggtgga aattgtctgg ttgatatcag acagcgccac
     2161 acccattgcc tcagctttcg cggcgttgac gttcactttg aacatcggcg tatcttccag
     2221 gccgttcggg cgtaccccgg tgacctgatt cggtgattgc gctgccagtg ataacagctc
     2281 gtttcgtgcc tgggttagtt tttcgtgccc caggttaccg ttgtccagca gttccatatc
     2341 aaaacctgac gcggtaccca gttcagccac cgcgggtaag ttgaacggga agacgacggc
     2401 tttattgata ctgcttagcg caatcattgc ccgctgaatg atcgcggtaa ccgagttttc
     2461 ctcaccgaca cgttcagacc acggcttgag actgataaac gccaggccgt tgttttgccc
     2521 ctggccgctg aagccaaagc cgccaacggt aaataccgac tggacattat ctttctcttt
     2581 agtcagataa taatccgtca cctgttgcag cactttcgtg gtgttaacca tggtggcacc
     2641 ggaaggtaac tgcgcggtgg tcataaatac cccctgatcc tcttctggta agaaagaggt
     2701 cggtgtacgc aggaacagca ccgccatccc ggcgcaaatc agcaggtaga ccaccatgta
     2761 gcgaccggta caatgcaaca gcgagcgggt gctatcggta tagtgctgag ttgatttttc
     2821 aaacagcgtg ttgaagcgtg cgaacagggc gttaggtttg tgaccgcctt ccggcgcggc
     2881 tttcagaatg gtggcgcaca gggcaggggt caggctcatt gccacaaata ctgaaagcag
     2941 catggaggag atcagcgtga tggagaactg gcggtagatc tccccggttg caccgctcat
     3001 aaaggccatc ggcataaaca ctgcggaaag aacaacggca ataccgacca gcgcacgttg
     3061 gatctgcccc atcgatttat gcgtcgcttc cttcggcggt agcttatctt ccgcaatgac
     3121 acgctcgacg ttctccacca ccacgatggc gtcatccacc agtaacccta tcgccagcac
     3181 catcccgaac atagtcaacg tgttaatggt gaaaccgacc gccgacaaga tcgcaaacgt
     3241 cccgagaata accaccggta cggcaatcgt cgggatgatt gtggcacgga aattctgcaa
     3301 aaacagatac atgaccagga agacgaggat gatagcctca accagtgttt tgaaaacttc
     3361 ctgaatagaa atttcgataa acggcgtggt gtcgtaagga taaaccgtct tcagacttgc
     3421 cgggaaatag gctgataagc ggttcagttc ctctttgact gcccgcgagg tatccagggc
     3481 gtttgctccg gtagccagtt tgatggcgat cccggcagca ggtttgccgt tatagcgcgc
     3541 cacggtggaa taatcttccg ccccaagttc gacgcgggcg acatcacgca gcagcacttg
     3601 cgaaccatct tgctgaactt tcaacaggat tttgccaaat tcttccggcg tttgcagacg
     3661 cgtctgcaca atgatcgagg cgtttagttg ctggtctgcc gcctgtggca tgccacccag
     3721 ttgaccacca gaaatctggt tgttttgcac cttaatctgg gaaataacat cggaaggtac
     3781 caggttatat ttattgagtt tttgcgggtc cagccagatc cgcatggcgt actcggaacc
     3841 aaagagctgt acgctaccaa cgcccgcggt acggcttagc gggtctttga tattagacgc
     3901 tacatagtcc gcgatatcgt actggtttag gctgccgtta tcagaaataa acgccgctac
     3961 catcaggata ttactgctcg atttatcgac gctaatcccc tgctgctgca ctgcttcagg
     4021 taatgaaggc atagcgagtt gcagtttatt ttgcacctga acctgtgcga tatcaggaga
     4081 tgtcccagtc tcgaaggtca gagtgataga ggcattaccc gccgcatcac tggttgaaga
     4141 catgtacatc aggccatcaa gcccattcat attttgctca atcacctgag tgaccgagtc
     4201 ttctaccgtt tgcgcatcgg cacccggata ggtagcgctg acggtaatgg tcggtggcgc
     4261 aatctgcgga tactgcgcaa ccggtaagtt catgatcgcc agaccacctg caagcatcat
     4321 aataatggca agtacccagg caaaaaccgg gcgatcaata aaatagttag ccatgaaagt
     4381 ccccttaagc ctgcaacgtt attgtttcga ttcggtgctg gcgttttcct ggctggagga
     4441 aattgctcgt gctttgatac ccggacgaat gcgttgcaaa ccggaaacga tcacccgatc
     4501 gccagcctgc aagccagagg tgacaaccca ctggtcgccg atggctttgc tggcttcaat
     4561 ttcgcgtagc tgcacgacat cgtctttatc cagaatgagc gccgttgctt taccctgggc
     4621 gttgtgggtg acgccttcct gcggcaccag taatacattc tggcggctac cttcatccac
     4681 taatgccgtg acatacatgc caggcagcaa gtcaccattt gggttgggga aaatcgcccg
     4741 taacgtcacg gagcccgtgg tttcatcaac cgtcgggtcg gagaatttca gcgtgccggt
     4801 ctggttgtaa cgtttaccat tttccagatt gagctgtact ggcgtactgc cctgaatctg
     4861 tttgatttgc ccactggcga cctcttcttt catgcgtaag aaatcttgca ccgactgcgt
     4921 gagatcgaca taaatcgggt ccagacgttg tacggtaacc agcgaatctg cctgattagc
     4981 ggtaacgagt gcgccgacgg tcaccgacga tttcccgctg acgcccgtaa tcggcgaggt
     5041 gacattcgcg tattgcagat tgatcgtcgc ctgttcaaca gccgctttgg cgacggtgac
     5101 attggcttct gcttcattca actgggtgcg cgcggtgtcg taatcctgac ggctaacgta
     5161 gttggtcttc agcaacgatg cctggcggtt aaaggtgatg cgggcattgc tggcggtaga
     5221 gagcgctttc gccagcgagc ctttggcgga gtttagctcg gcctgtaaag gtgcaggatc
     5281 aatctgatac agcgaatcgc cctggttcac tttatcgcct tcgataaagt tgcgtttaat
     5341 gataataccg cccacctggg gacgtatctc ggcaacttca taaggaacgg ttctaccggg
     5401 caattcgctc aacacattga ccgacccagg ggagagtgtg acgacaccga cctcaggcgt
     5461 catggcggcg gtgttttccg ccgatttgtc atcgcaggcg gtgagcatcg cgccgcagaa
     5521 taacaacggt attaacagct ttcttcttct gttcatttta gtccctgaaa attcttgaga
     5581 aataacggtt ctttaaattg cgcgcgagga gagatatgag ggatttacag accagcggca
     5641 tagtggggtt gtcagccagt aaggtgggat acaggcatag tgatcgacat ggtgaggtca
     5701 acgacaaaaa aaatgttgcc gttctgccaa cagttcctgc cagcattcgg gcaacgaagg
     5761 gttctactgg tggatacaca taccagggga ataaagtcgt tttagcctct cctttagtaa
     5821 tcaccggggg taatgaaatc agcatctgca tccctcgtca tgccagccat caatttcagt
     5881 tgcttatgtc ctgactaaaa ataagatgtg atacccaggg tgacgatgtc gctcatacgt
     5941 cttagtttca gcttgtacat aatgttgaat ttctgaatct ttaat
//
LOCUS       DAZUCE010000270         5943 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZUCE010000270 DAZUCE010000000
VERSION     DAZUCE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.271"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            1..1037
                     /gene="ygjJ"
                     /locus_tag="ACHY03_004547"
                     /pseudo
     CDS             1..1037
                     /gene="ygjJ"
                     /locus_tag="ACHY03_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1054..3405
                     /gene="ygjK"
                     /locus_tag="ACHY03_004548"
     CDS             1054..3405
                     /gene="ygjK"
                     /locus_tag="ACHY03_004548"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFS3907700.1"
                     /translation="MKIKTILTPVTCALLISFSAHATNADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFIASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGLIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            3930..>5943
                     /gene="fadH"
                     /locus_tag="ACHY03_004549"
     CDS             3930..>5943
                     /gene="fadH"
                     /locus_tag="ACHY03_004549"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFS3907701.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAMQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHLKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT         1420 a         1612 c         1676 g         1235 t
ORIGIN      
        1 ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt ccggcagaac
       61 atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt cgtttttacg
      121 gcgaattagg ggttggtgga tatatggatt tagagggtga gaataaacat aaatacagcg
      181 acggcaccta tattgaaggt ggactggaga tgaagtacgg ctcctagttc ggcctgattt
      241 acggcgaagg ctggaccgta caggccgacc acgacggtaa tgcctgggtg cctgaccata
      301 gctggggtgg cttcgaaggt gggattaacc gtttctatgg cggttatcgt accaatgatg
      361 gcaccgaaat catgctcagt ctgcgtcagg attcctcgct ggatgaccta caatggtggg
      421 gcgacttcac acccgatttg ggctacgtca ttcccaatac ccgcgacatt atgaccgcgc
      481 tgaaggtaca gaacttaagc ggcaactttc gttatagcgt caccgccaca cctgccggac
      541 atcatgacga aagcaaagcc tggctacatt tcggcaagta cgatcgctac gacgacaaat
      601 acacctatcc ggcaatgatg aacggttaca tccagtatga ccttgccgaa ggcatcacct
      661 ggatgaacgg tctggaaatc accgacggca caggacaact ctatctcacg ggcctgctta
      721 cccctaactt tgccgctcgc gcctggcacc ataccggacg tgccgacggg ctggacgtac
      781 cgggaagtga aagcgggatg atggtgagcg ccatgtatga agcgttaaag ggcgtttatc
      841 tctccaccgc ttacacctac gccaaacatc gccctgacca cgctgacgat gaaaccacct
      901 ctttcatgca gtttggtatc tggtacgaat atggcggcgg acgtttcgca acggcttttg
      961 atagccgctt ctacatgaaa aatgcttcta acgatcccag cgaccaaatc ttcctgatgc
     1021 aatatttcta ctggtaataa ggactgtaat ttcatgaaaa tcaaaactat tttaacgcca
     1081 gtaacctgcg ctctgctgat aagtttttcc gcccatgcca ctaacgccga caattataaa
     1141 aacgtgatta accgtactgg tgcgccgcag tacatgaagg attacgatta cgacgatcac
     1201 cagcgtttta atccgttttt cgatctcgga gcctggcatg gtcatctgtt gccagacggc
     1261 cctaacacca tgggcggttt tccaggcgtt gcgctgctga cggaagagta catcaacttt
     1321 atagccagca atttcgaccg actgaccgtc tggcaggacg gcaagaaagt cgacttcacg
     1381 ctggaggcat acagtattcc cggcgcgctg gtgcaaaaac tgacagcaaa agatgtgcag
     1441 gtcgaaatga ctctgcgctt cgccacgccg cgcacgtcac tactggaaac taaaatcatc
     1501 agcaataaac cgctggatct ggtgtgggat ggcgaactgc tggaaaaact ggaagcgaaa
     1561 gaagggaaac cgctttccga taaaaccatt gctggcgaat accctgacta ccagcgcaaa
     1621 atcagcgcca cccgtgatgg cctgaaagtc acctttggca aagtgcgcgc cacttgggat
     1681 ctgctgacct ccggtgaatc agaatatcag gtgcataaat ccctgccggt gcagactgaa
     1741 atcaacggta atcgctttac cagtaaggcg catatcaacg ggtcgacgac gctctatacc
     1801 acctactccc acctgctgac cgctcaggaa gttagcaaag agcaaatgca gatccgcgat
     1861 attctggcgc gtccggcgtt ttacctcacc gcctcgcagc aacgctggga agagtatctg
     1921 aagaaagggt taaccaatcc ggatgcgacg ccggaacaga cgcgcgtcgc ggtgaaagcg
     1981 atcgaaacgc tcaacggtaa ctggcgctcg cctgggggtg cggtgaaata taacaccgtc
     2041 acgccgtcgg tgaccgggcg ctggttctcc ggcaatcaga cctggccgtg ggatacctgg
     2101 aagcaggcgt ttgcgatggc gcatttcaat ccggacatcg ccaaagagaa tatccgcgcg
     2161 gtcttctcct ggcagatcca gcctggcgac agcgtgcgtc cgcaggatgt gggctttgtc
     2221 cccgacctga tagcgtggaa tcttagcccc gagcgtggtg gcgatggtgg caactggaac
     2281 gaacgaaata ccaaacccag ccttgccgcc tggtcggtga tggaagtgta caacgtcacc
     2341 caggataaaa cctggctggc agagatgtac ccgaaactgg tggcctatca cgactggtgg
     2401 ttacgtaacc gcgatcacaa cggcaacggc gtgccggaat atggcgcgac ccgcgacaaa
     2461 gcccacaaca ctgagagcgg cgagatgctg tttacggtga agaaaggcaa caaagaagag
     2521 acgcagtctg ggctgaacaa ctacgcccgc gtggtggaga aaggccagta cgacagtctg
     2581 gaaattccgg cacaggttgc tgcatcgtgg gaatcggggc gtgatgatgc cgccgtcttt
     2641 gggtttatcg acaaagaaca gctggataaa tatgtcgcaa gcggcggcaa acgtagcgac
     2701 tggacggtga aattcgccga aaaccgcagt caggacggaa cgttgctggg gtactcgcta
     2761 ttgcaggagt cggtggatca ggccagctat atgtacagcg ataaccatta tctggcggag
     2821 atggcgacga ttctcggtaa accggaagag gctaagcgct atcgccagtt ggcacagcag
     2881 ctcgcggact acatcaacac ctgtatgttc gacccggcta cgcagtactt ctatgacgtg
     2941 cgtattgaag ataaaccgct ggcgaacggc tgcgcgggca aaccgattgt tgagcgcggt
     3001 aaagggccgg aaggctggtc gccgctgttt aacggtgcgg caacgcaggc caatgccgac
     3061 gcggtggtga aggtgatgct cgatcctaaa gagttcaaca cctttgtccc gctgggaacg
     3121 gcagcgttaa ccaacccggc ctttggcgcg gatatctact ggcgcgggcg cgtatgggtg
     3181 gatcagttct ggtttggtct gaaagggatg gagcgttacg gttatcgcga tgatgccctg
     3241 aagctggcgg atacgttctt ccggcatgcc aaaggattaa ccgccgatgg cctaattcag
     3301 gagaattaca acccgctgac tggcgcgcag caaggcgcac caaatttctc ctggagtgcc
     3361 gcacatttgt atatgttgta taacgatttt ttccgtaagc aataatttgc aacggctggc
     3421 ggatgcggcg taaacgcctt atccgcccta catgtatgtt ccccaggccg gataagacgc
     3481 gacaagcgtc gtatccggca tctgcaccgc gccaccggcg gatgctgcgt gaacgcctta
     3541 tccgccctac atgttcgttc ccgtaggctg gataagacgc gacaagcgtc gcatccggca
     3601 tctgcacctc gccactggcg gatgcggcgt gaacgcctta tccgccctac atgttcgttc
     3661 ccgtaggctg gataagaccc gacaagcgtc gcatccggcg tctgcaccgc gccactggcg
     3721 gatgcggcgt gaacgcctta tccgccctac atgtgcaatc ccccaggtcg gatagggccc
     3781 ggcaagcgtc gcatccggcg ttaccaccgg gcgtattctt tttgaatccc atcacaaacc
     3841 ccgcactccc cttttccctt ttctccggcg acggctaaat tagaactcat ccgaccacat
     3901 aacaattatt ttacatactg gacaatttta tgagctaccc gtcgctgttc gccccgctgg
     3961 atttaggttt taccacgtta aaaaaccgcg tgttgatggg ttcaatgcac accgggctgg
     4021 aggaataccc ggacggtgct gaacggctgg cagcgtttta tgccgaacgc gcccgtcacg
     4081 gcgtggcgct gattgttagc ggcggcattg caccagattt aacaggcgtt ggcatggaag
     4141 gcggcgcaat gctcaatgac gccagccaga tcccacacca tcgcaccatt accgaagccg
     4201 tacatcagga aggcggcaaa atagccctgc aaattttgca taccgggcgc tacagctacc
     4261 aaccgcatct ggtcgctccc tccgcattgc aggcccccat caaccgtttc gttcctcacg
     4321 aactcaccca tgaagagatc ctgcaactga tcgacgattt cgcccactgc gcgcaactgg
     4381 cgcgggaggc aggatacgac ggtgtagagg tgatgggttc cgaagggtat ttgatcaacg
     4441 aatttctgac gctgcgcacc aatcagcgta gtgaccagtg gggcggcgat taccgcaacc
     4501 ggatgcgatt tgccgttgaa gtggtgagtg cggtacgcga acgcgttggc aacgacttca
     4561 ttattatcta ccgactgtcg atgctcgacc tggtcgaaga cggcgggact tttgccgaaa
     4621 cggtagagct ggcgcaggcc attgaagcgg ctggcgcaac cattatcaac accggcattg
     4681 gctggcatga agcgcgtatt ccgaccatcg ccacgcccgt gccacgcggc gcatttagct
     4741 gggtcacgcg caaactgaaa ggtcacgtct cgctgccgct ggtgaccacc aaccggatta
     4801 acgatccgca ggttgccgac gatattctct cgcgcggcga tgccgatatg gtatcgatgg
     4861 cgcgaccgtt tcttgcggat gcggaactgc tgtcaaaagc gcaatcaggg cgagccgatg
     4921 agatcaacac ctgcattggc tgcaaccagg cgtgtctcga tcagatcttc gttggcaagg
     4981 tcacctcgtg tctggtgaat cctcgcgcct gccatgaaac caaaatgccg atccttcccg
     5041 ccatgcagaa aaaaaatctg gcggtggtcg gtgcgggacc agctgggctg gcgtttgcca
     5101 ttaacgcggc ggcgcgtggg catcaggtaa cattgtttga tgcccacagc gagatcggcg
     5161 ggcagtttaa tatcgccaaa cagatccccg gcaaagagga gttttacgaa acgctgcgct
     5221 attaccaccg gatgatcgaa gtgacgggcg tgacgctaaa actcaatcac accgtgacgg
     5281 cggatcagtt acaggcgttc gatgaaacga tcctcgccag tgggatcgtg ccacgcgttc
     5341 cgcctatcga cgggatcgat catctgaaag tattgagtta tctcgatgta ctgcgcgaca
     5401 aagcgccggt gggcaacaaa gttgccatca tcggttgtgg tgggattggc tttgatacgg
     5461 cgatgtattt aagtcagccg ggcgaatcca ccagccagaa tatcgctgag ttttgtaatg
     5521 agtgggggat cgacagtagc ctacaacagg ctggtggctt aagcccgcag ggaatgcaga
     5581 tccctcgtag cccacggcag attgtgatgc tccagcgcaa agccagcaaa ccgggacagg
     5641 ggttaggcaa aaccaccggc tggatccatc gcaccaccct gctctcgcgg ggtgtgaaaa
     5701 tgatcccagg cgtaagttat cagaagattg acgatgacgg gctgcatgtg gtgatcaacg
     5761 gcgaaacgca ggtattagca gtggacaatg tggtgatctg cgcagggcaa gagccaaacc
     5821 gcgcgctggc gcagccgctg attgatagcg ggaaaacggt gcatttaatt ggcggctgcg
     5881 atgtggctat ggagctggac gcacgacggg caattgccca gggaacacgg ctggcgctgg
     5941 aga
//
LOCUS       DAZUCE010000271         5887 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZUCE010000271 DAZUCE010000000
VERSION     DAZUCE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.272"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(94..621)
                     /gene="mobB"
                     /locus_tag="ACHY03_004550"
     CDS             complement(94..621)
                     /gene="mobB"
                     /locus_tag="ACHY03_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFS3907702.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(603..1187)
                     /gene="mobA"
                     /locus_tag="ACHY03_004551"
     CDS             complement(603..1187)
                     /gene="mobA"
                     /locus_tag="ACHY03_004551"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFS3907703.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLATRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1257..1526
                     /locus_tag="ACHY03_004552"
     CDS             1257..1526
                     /locus_tag="ACHY03_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFS3907704.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1603..2589
                     /gene="srkA"
                     /locus_tag="ACHY03_004553"
     CDS             1603..2589
                     /gene="srkA"
                     /locus_tag="ACHY03_004553"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFS3907705.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2606..3232
                     /gene="dsbA"
                     /locus_tag="ACHY03_004554"
     CDS             2606..3232
                     /gene="dsbA"
                     /locus_tag="ACHY03_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFS3907706.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            3387..4817
                     /locus_tag="ACHY03_004555"
     CDS             3387..4817
                     /locus_tag="ACHY03_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFS3907707.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEGIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMHKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(4858..5790)
                     /locus_tag="ACHY03_004556"
     CDS             complement(4858..5790)
                     /locus_tag="ACHY03_004556"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFS3907708.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
BASE COUNT         1642 a         1327 c         1373 g         1545 t
ORIGIN      
        1 gcgaaaggcc atccgtcagg atggcctttt tgcgttggtg cagaaaaaaa tgccggatgt
       61 gacgctggcg cgtcttatcc agcctactct tgcttatccg ttctgctttt gcatccactc
      121 caccacaaaa tcagccagcc cctcaacatc attaatatcc agtaatgcga catcaagatt
      181 aagcggcaca tcactggcta cagcaataac atgcctgtct atcactaatt cttcaggtcg
      241 atgtccggct ccatcgcgaa acagcacaat ctttgcgatc tcttcatgct taaacccttc
      301 gaccagaatc aaatccagct ttgaggtatc cattcgactt gcgagaaact gtagatccag
      361 ctcttcttcg tctggtgttt ccgtcatcaa ggcccatcgt tgctggctgg caacgatggt
      421 ttgtgccgcg ccagccttgc gcagctcata gctatctttg cctggcttat caacatccat
      481 atcatggtgc gtatgcttaa tcagccctgg acggatccct ctggcgcata atgccgggat
      541 cagttttttc aaaagcgtag tttttccggt accactccac gcggcaaagg cgagtaacgg
      601 tatcatcgtt tttcctgcca tcgggcaagc tcctctggcg tattcacgtt aacaaatgca
      661 tctttatgat cgctgaaatc aaccgcatgc ccgccagcca gacgcataaa tgccattacc
      721 cggcgttctc ctgcttgcag atattccagc aataaaggct caatagcgcg gtttaccaga
      781 gcaatagtcg ggtgatcgcg ttcaccgtca tggacccaca cgacaggcgc atctttgcgc
      841 tgatgattaa gccgggtggc taaatcatgg ggaatgtaag gcgtatcgca cggacaaaac
      901 aaaaaccact caccagcttc ctgctgcatt actgaaagca tccctgccag agggcctggg
      961 taatccgcca gtgaatcttc aatcactttc agaccgcctg cctgatagat ttcctgatga
     1021 cgattagcat taaccacgac gtgagagagc tgcgtcataa gcgcgtcagc gacatgttgc
     1081 cacagcggtt tgccatttaa gtccagcaat cctttatcta cgccgcccat tcgtctggct
     1141 ttaccgcctg ccagcacaac gcctgttatc gtcgtcatca gattcaccga tatcgcctct
     1201 tttattgtgg gattgaccct gctaacgtgt ctgtctcaag agtaaggagc attactatga
     1261 aatgtaaacg tctgaatgaa gttattgaac tcctccagcc agcctggcaa aaagagccag
     1321 accttaacct gttgcaattt ttgcagaaac tggcgaaaga gtcaggtttt gacggcgaac
     1381 tggcggattt gacggatgac attctgatct atcacctgaa aatgcgtgat tccgcgaaag
     1441 atgcggtgat cccgggtttg cagaaagatt atgaggaaga cttcaaaacg gcgctgttac
     1501 gcgctcgcgg cgtaattaaa gagtaaaagc ttgtaagcgg cgccaccaaa atcatcgtga
     1561 aatgatatcc ttcatcattc gtaatgtttt ccggatgatg ggatgaacaa cagcgctttt
     1621 actttccaga cactacaccc ggataccatc atggacgctc tgtttgagca agggatccgg
     1681 gtggattccg gtcttacccc gcttaacagc tatgaaaacc gtgtctatca atttcaggac
     1741 gaagatcgtc gacgttttgt cgtcaaattt tatcgccctg aacgttggac agccgatcaa
     1801 atcctcgaag aacatcaatt tgcgttgcag ctggtaaatg atgaagttcc ggtcgcagca
     1861 cctgtggctt ttaacggtca gactttattg aatcatcagg gattttattt cgctgttttt
     1921 ccaagcgtcg gtggtcgcca gttcgaagct gataatatcg atcagatgga agcggttggg
     1981 cgttatttag ggcgtatgca ccagacgggg cgcaaacagc tttttatcca tcgcccgacc
     2041 atcggtctga atgaatatct cattgagcca cgcaagctgt ttgaggacgc tacactgata
     2101 ccttccgggt tgaaagcggc attcctgaaa gcgacagatg agctgatcgc cgccgttaca
     2161 gcacactggc gggaagattt caccgttctg cggctacatg gagactgcca cgccgggaat
     2221 attctctggc gcgatggtcc aatgtttgtt gatctggatg atgcacgtaa tggtccagct
     2281 attcaggatt tgtggatgtt gctcaatggc gataaagccg agcagcggat gcaactggaa
     2341 actattattg aagcttatga agaatttagc gagttcgaca ccgctgaaat cggactgatt
     2401 gaacctttac gcgccatgcg tttggtttat tatcttgcct ggctaatgcg gcgttgggct
     2461 gatcccgcgt tcccgaaaaa tttcccgtgg ttaaccgggg aagattactg gctacgacag
     2521 acggcgactt ttatagaaca ggcaaaagtt ctacaagaac cccctttgca attaacacct
     2581 atgtattaat cggagagagt agatcatgaa aaagatttgg ctggcgctgg ctggtttagt
     2641 tttagcgttt agcgcatcgg cggcgcagta tgaagatggt aaacagtaca ctaccctgga
     2701 aaaaccggta gctggcgcgc cgcaagtgct ggagtttttc tctttcttct gcccgcactg
     2761 ctatcagttt gaagaagttc tgcatatttc tgataatgtg aagaaaaaac tgccggaagg
     2821 cgtgaagatg actaaatacc acgtcaactt catgggtggt gacctgggca aagatctgac
     2881 tcaggcatgg gctgtggcga tggcgctggg cgtggaagac aaagtgactg ttccgctgtt
     2941 tgaaggcgta cagaaaaccc agaccattcg ttctgcttct gatatccgcg atgtatttat
     3001 caacgcaggt attaaaggtg aagagtacga cgcggcgtgg aacagcttcg tggtgaaatc
     3061 tctggtcgct cagcaggaaa aagctgcagc tgacgtgcaa ttgcgtggtg ttccggcgat
     3121 gtttgttaac ggtaaatatc agctgaatcc gcagggtatg gataccagca atatggatgt
     3181 ttttgttcag cagtatgctg atacagtgaa atatctgtcc gagaaaaaat aattcaatgt
     3241 aaattacata aggcccgtga atattcacgg gcttttttta ttatttaata aatataaata
     3301 cattctgata atgcatcctg ccgctggaca ttatttcagc ataagtggat attcaaagtt
     3361 ttgctgtttt atcagggaat atttatatga tacgtaagtc agctacaggt gttattgttg
     3421 cgttagccgt aatctggggt ggtggcacat ggtacacagg tacgcaaatt cagcctggtg
     3481 tcgaaaaatt tattaaagat tttaacgatg ctaaaaagaa aggtgaacat gcctacgata
     3541 tgacgttaag ttatcaaaat tttgacaaag gcttttttaa ttcccgtttt caaatgcaaa
     3601 tgacattcga taacggtgca cctgatctca atatcaagcc aggccagaaa gttgtatttg
     3661 atgtggatgt tgagcacggt ccgttgccca tcacaatgtt aatgcatggt aatgtcatcc
     3721 cagcactggc agcggcaaaa gtgaacttag tgaataatga actgacacaa ccgctattta
     3781 tcgccgcgaa aaataaatcg cccgtggaag cgacattgcg attcgcgttt ggtggctcat
     3841 tctctacgac attagatgtt gcccctgcag agtatggaaa gttttctttt ggtgagggcc
     3901 agtttacttt taatggtgat ggtagttcat tgtctaacct ggatattgaa ggcaaagtcg
     3961 aaggtattgt tctgcaatta tcaccaatga acaaagtaac ggcaaaaagt tttaccattg
     4021 attctctggc gcgattagaa gaaaagaaat ttccggttgg tgaaagcgag tcgaaattta
     4081 atcagattaa cattatcaat cacggggaag acgttgccca aatcgatgct ttcgttgcaa
     4141 aaaccaggct ggatcgcgtt aaagacaaag attatatcaa tgtcaatctg acctacgaac
     4201 ttgataagtt aacaaaaggg aatcagcaac tcggtagtgg tgagtggtca ttgattgctg
     4261 aatctattga tccctcagca gtgcgccaat ttatcatcca gtataacatt gcgatgcata
     4321 agcagcttgc tgcacaccct gagttagcaa acgatgaagt tgctctgcaa gaagtgaatg
     4381 ctgcattgtt caaagagtat ttaccgttat tacaacaaag tgagccgacc attaaacaac
     4441 cggtaagatg gaagaacgca ctcggcgaac taaatgccaa tctggatatc agtattgccg
     4501 acccagccaa atcttcatca tccacaaaca aagatatcaa atcgctcaat tttgatgtga
     4561 agttaccgct taatgtcgtc acagaaaccg caaaacagct taatttatct gaaggaatgg
     4621 atgcggaaaa agcgcaaaag caggctgata aacaaatcag cgggatgatg acattaggtc
     4681 agatgtttca gttaatcacg attgacaaca ataccgcctc gctgcagctg cgttatacac
     4741 cgggtaaagt tgtttttaac ggacaggaga tgagcgaaga agaatttatg tctcgtgccg
     4801 gacgttttgt tcattaaaat actcactcgc ctctgctcat tcagaggcga gtaaaactta
     4861 cttattctga cgctgtgatg acatgagaga cgtcagaagc cggtcttttt cctgccacag
     4921 tgagttcagc cattgctgaa aatggcgctt aaagcttttg tcattgatgt agtcgccatg
     4981 taactcatcg gcaataggct gtaaatccac atgtacgaca atccgcgtca gtttaccgct
     5041 taacatatcg aagaacggct gacggttatt gtccggataa cacagcgtaa cattaagcag
     5101 tttatcgaat tgtttaccca gtacatttag cgccatcgca atgcctgccg cctttggtgg
     5161 caacaagttt tgaaaagtgg agtgggtttg ctgatgtttt tcttgcgtga aacgggagcc
     5221 ttcaacgaaa ttgacaatgg tggtgggatg caggcgaaac ttttcacaag aacgccgagt
     5281 ggtttcaaca tctttaccac gtcgctccgg atgacgtaat aaataagcgc gggaataacg
     5341 cttcataaac ggcatatcca gcgcccagca cgccaggcca agaaatggca cccaggccag
     5401 ctgttgcttg aggaaatatt tattcatcgg aatgtgctta cgaaacagca cgcacagtac
     5461 gacaatatct gcccaactac ggtggttaca aataagcaga taccagttct tcttacttaa
     5521 cccttccagc ccgtgaactt cccattgcag gtgtgggttc agatgcagta ataccgccag
     5581 accttcacac cagcaataca tcataaaatc acaaaaacgc gaaacctttc gccaaatgac
     5641 tggcacaggt agcaatagtt ttacaatccc ggcaatgatg atcgggacgg aacaaaaaat
     5701 agtgaccaaa atggtcaata cgatactcag caaaagggtt atcgcagcga gtattctcgt
     5761 cataatgaat ttattcaaaa ggttagccat agacagtgcg ctaaagaagc gcaaggcgca
     5821 gattttagca gaaaacgacc caaataacgg atgatcctta aggagaaaaa taattcatat
     5881 ctatcca
//
LOCUS       DAZUCE010000272         5882 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZUCE010000272 DAZUCE010000000
VERSION     DAZUCE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.273"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            134..781
                     /gene="fucA"
                     /locus_tag="ACHY03_004557"
     CDS             134..781
                     /gene="fucA"
                     /locus_tag="ACHY03_004557"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFS3907709.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            809..1957
                     /gene="fucO"
                     /locus_tag="ACHY03_004558"
     CDS             809..1957
                     /gene="fucO"
                     /locus_tag="ACHY03_004558"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFS3907710.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTDEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2012..2767)
                     /gene="xni"
                     /locus_tag="ACHY03_004559"
     CDS             complement(2012..2767)
                     /gene="xni"
                     /locus_tag="ACHY03_004559"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFS3907711.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(2879..4246)
                     /gene="sdaB"
                     /locus_tag="ACHY03_004560"
     CDS             complement(2879..4246)
                     /gene="sdaB"
                     /locus_tag="ACHY03_004560"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFS3907712.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4304..5593)
                     /gene="sdaC"
                     /locus_tag="ACHY03_004561"
     CDS             complement(4304..5593)
                     /gene="sdaC"
                     /locus_tag="ACHY03_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFS3907713.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1474 a         1471 c         1572 g         1365 t
ORIGIN      
        1 aggtcacaaa atataagctt gtgaatttct ttcttgtgaa tcaggaagaa gatggtgggt
       61 aattaaacgg ctaattcaat agtgtgaaag gaacaacatt attgccctgt tttgaatcag
      121 agagaggtag ctaatggaac gaaataaact tgctcgtcag attattgaca cttgcctgga
      181 aatgacccgc ctgggactga accaggggac agcggggaac gtcagtgtac gttatcagga
      241 tgggatgctg attacgccta caggcattcc atatgaaaaa ctgacggagt cgcatattgt
      301 ctttattgat ggcaacggta aacatgagga aggaaagctc ccctcaagcg aatggcgttt
      361 ccatatggcg gcctatcaaa gcagaccgga tgccaacgcg gttgttcaca atcatgccgt
      421 tcattgcacg gcagtttcca ttcttaaccg atcgatcccc gctattcact acatgattgc
      481 ggcggctggc ggtaattcta ttccttgcgc gccttatgcg acctttggaa cacgcgaact
      541 ttctgaacat gttgcgctgg ctctcaaaaa tcgtaaggca actttgttac aacatcatgg
      601 gcttatcgct tgtgaggtga atctggaaaa agcgttatgg ctggcgcatg aagttgaagt
      661 gctggcgcaa ctttacctga cgaccctggc gattacggac ccggtgccag tactgagcga
      721 tgaagagatt gccgtagtgc tggagaaatt caaaacctat gggttacgga ttgaagagta
      781 atttcgtaaa gcaacaagga gaaggatgat ggctaacaga atgattctga acgaaacggc
      841 atggtttggt cggggtgctg ttggggcttt aaccgatgag gtgaaacgcc gtggttatca
      901 gaaggcgctg atcgtcaccg ataaaacgct ggtgcaatgc ggcgtggtgg cgaaagtgac
      961 cgataagatg gatgctgcag ggctggcatg ggcgatttac gacggcgtag tgcccaaccc
     1021 aacaattact gtcgtcaaag aagggctcgg tgtattccag aatagcggcg cggattacct
     1081 gatcgctatt ggtggtggtt ctccacagga tacttgtaaa gcgattggca ttatcagcaa
     1141 caacccggag tttgccgatg tgcgtagcct ggaaggactt tccccgacca ataaacccag
     1201 tgtaccgatt ctggcaatcc ccaccacagc aggcactgcg gcagaagtga ccattaacta
     1261 cgtgatcact gacgaagaaa aacggcgcaa gtttgtttgc gttgatccgc atgatatccc
     1321 gcaggtggcg tttattgacg ctgacatgat ggatggtatg cctccagcgc tgaaagctgc
     1381 gacgggtgtc gatgcgctca ctcatgctat tgaggggtat attacccgtg gcgcgtgggc
     1441 gctaaccgat gcactgcaca ttaaagcgat tgaaatcatt gctggggcgc tgcgaggatc
     1501 ggttgctggt gataaggatg ccggagaaga aatggcgctc gggcagtatg ttgcgggtat
     1561 gggcttctcg aatgttgggt tagggttggt gcatggtatg gcgcatccac tgggcgcgtt
     1621 ttacaacact ccacacggtg ttgcgaacgc catcctgtta ccgcatgtca tgcgctataa
     1681 cgctgacttt accgatgaga agtaccgcga tatcgcgcgc gttatgggcg tgaaagtgga
     1741 aggtatgagc ctggaagagg cgcgtaatgc cgctgttgaa gcggtgtttg ctctcaaccg
     1801 tgatgtcggt attccgccac atttgcgtga tgttggggta cgcaaggaag acattccggc
     1861 actggcgcag gcggcactgg atgatgtttg taccggtggc aacccgcgtg aagcaacgct
     1921 tgaggatatt gtagagcttt accataccgc ctggtaaatg cgctgatgtg ataatgccgg
     1981 atacgacgtt tgcggcgtat ccggctcgcc gttaccgtac caatcgcaat tgctgtaaat
     2041 tgccatcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga aacgccatct
     2101 ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga ttctcatata
     2161 tcccttccag actctgaaac tcgaccagca gctgcgtggc gctttttggt ccgattcccg
     2221 caacacccgg tacctttgaa ctactgatcc ccgcaagtcc ccagtaatcg ggcaactgct
     2281 gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt ttctggaagt
     2341 aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct gtcgaaacaa
     2401 tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag cgtggcggct aagtcatcgg
     2461 cttcgttgcc gctggttgac cagcacggga cgccgcgttg ctcaaaggcg gcgcgtaatg
     2521 caggcatctc gtcgtgcaac tcttccggca ttggcggtcg acccgctttg taatctggta
     2581 aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg acagcgtggg
     2641 ttggctggct gtgcataatg agctgatcga gcgcgtgctg gcaggtttcg acacagggcg
     2701 aaccctgaac ggcatgaatg cgacgaataa gattcagtgc atcgacaata agcaaatgaa
     2761 cagccacgat aaccccctta aagatcaggc gataagggta acattcgata ctggaacagg
     2821 ctatggctgg atgagaaatc gggaagaggc ctcgcaaaaa gaggcctctg gagagcgatt
     2881 aatcgcaggc aacgatcttc attgccaggc cgccgcgaga ggtttcgcgg tacttggcgt
     2941 tcatatcttt acctgtttcg tacatggttt cgataacttt atcgaggcag acgcgcggct
     3001 cgctggtacg gcgcagcgcc atacgtgcgg cgttcaccgc ttttactgcc gcaatggcgt
     3061 tacgctcgat gcatggcacc tgtacctgtc cggcgaccgg gtcacacgtc agaccgaggt
     3121 tgtgctccat ggcgatttcc gccgcgatgc acacctgcgc cgggcttgcg cctaatagtt
     3181 ctgccagacc agccgccgcc attgagcacg ccacaccaac ttcaccctgg caacccactt
     3241 cggcaccaga aatcgacgcg ttcatcttat aaagagaacc aatggcgctg gctaccagca
     3301 ggtaacgagc cagtgagtta gcgttcactt cgcggataaa cttatcgtag tacgccagca
     3361 ctgccgggat aatcccgcac gcaccgttag tcggcgcagt caccacgcga ccgccagcag
     3421 cgttctcttc gttcactgcc agtgcaaaca tgttgatcca gtcaacaacc gccatcgggt
     3481 cagtggtggt tttatcctgg ctgaccagca tccggcgtag tgccgcagca cggcgtggaa
     3541 cacgcagttt gccaggtaac acaccttcgg tggaaatacc gcgctcaata ccgccacgca
     3601 tcacttccca gacgttcgcc aggtgctgtt ccagctcttc tttgctgtgc agcgccagct
     3661 cgtttttcat catcaggcca gagagtgaca gcccggtttc ctgacaatgt ttttgcagat
     3721 cggctgctga actgtacgga taaggaactt caaccggtgc gctatcctgc tggccaaaat
     3781 gctcttcatc aacgataaag ccaccgccaa tagagtagta agtctgactg taaacgactt
     3841 tatcacccgc cagcgcggta atgcgcatac cgttttcatg cagagaaagg ttgtctgcat
     3901 ggaagttcat gcactgatca accgggaact ccacttcatg ctgaccgttt gccagcatca
     3961 ggcgaccatg agtattcaca tcctgaataa aaccagggat ggaatcgata tccacggtat
     4021 ccggcaggtt acccgccagg cccataataa tggcgatatc agtgtggtgg cctttaccag
     4081 tcagagagag cgagccgtac acgtcaacca ccacgcgggt cacgtcttta agcaggttac
     4141 gggcaatcag atcgtcggta aattgtttac ccgctttcat tggtccaacg gtatgagaac
     4201 tggaagggcc aatgccgatt ttgaaaatat cgaatacgct aatcatagga aatacatcgc
     4261 gttaaaacgg aggaagcgcc gcccgaaagc ggcgcgaaag gacttagctg aacagagagt
     4321 agaagattgc ggagattgca atcagaccca tcacgacaac gaatacgttg ctgatgtgac
     4381 cgctgtactt acgcattgcc ggtactttct gaattgcgta catcggcatc aggaacagga
     4441 tcatcgcgat gattgggccg cccagggttt caatcatacc caggatgctc gggttcaggg
     4501 tggcaacaat ccaggtcgtt accagcatga acagcgcagt gatacggttc agcttgttga
     4561 tttcgataga cttaccttta ccacgcagag atttaatcac cataccgttg aagccttcac
     4621 gtgcgcccag gtagtggccg aggaaggatt tggtgatagc gataatcgcg ataatcggag
     4681 ccatccacgc gataaccggt gcgttaaagt ggttagccag gtaagacaga atcgagatgt
     4741 tctgctcttt agccgcagcc aggtctgccg gagtcaggct cagtacgcag ctgaagacga
     4801 agaacattac ggtcagcacc atcatgatgt gtgcgaatgc caggatcttc gagcatttct
     4861 gttctgccat atcgccgtac tcttcacgct tcgcaacggc gaaagaagag atgatcggag
     4921 agtggttgaa cgagaacacc attaccggaa ttgccagcca cagggtcatc cacagaccgt
     4981 ttccggttgc agatgcagtg tccagagaca gcgtttccag tgctgcgccg ttccactgcg
     5041 ggatcaggta cagagccagc agcatcaata cgccaacaaa cgggaacacc agaatactca
     5101 tcgctttaac gatcatctgc tcaccgaagc gaacgatggt catcataccc acgatcagga
     5161 tcagcgacag aatcgcacgt ggcggtggcg tcatacccag ctggtgagac atgaagcttt
     5221 caactgtatt ggtgattgcc acgctataaa ccagcaggat cgggtagata gcgaagaagt
     5281 agagcagggt aatcagttta cctgcgccaa taccaaagtg ttcttctaca acctcggtga
     5341 tgtcttcgcc cgggttttta ccagacagta cgaagcgagt caggccgcgg tgagcaaaaa
     5401 acgtcatcgg gaacgcaagg atagccatga tgatcagcgg gatcataccg ccaacaccgg
     5461 cgttgattgg caggaacagc acgcccgcgc cgattgccgt gccgtaaagg cccagcatcc
     5521 acatggtgtc tgtcttgcgc caggcactac gagagtcttt cgacgcaatc gtgctggttt
     5581 gagtcgtttc catctatttc tcctggagga atgcaaataa ttcagctttt agccagttca
     5641 gcccgggaag gtgcctgacc gtaaaaatga aatctgtcta tcggcgatgg cttaatcatt
     5701 taccgccgta ataatttgag ggcggaaaga tacacacaag ttatgtatct atcagtgatc
     5761 ttgatcccaa atacaataat ctacttggaa gatagattat cccaacgctt tactctgtaa
     5821 atcaaaagta aaagagaatt taagggcaat atttgcccat taaacgacat ctgacttctt
     5881 cc
//
LOCUS       DAZUCE010000273         5871 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZUCE010000273 DAZUCE010000000
VERSION     DAZUCE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.274"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(41..232)
                     /gene="yaiA"
                     /locus_tag="ACHY03_004562"
     CDS             complement(41..232)
                     /gene="yaiA"
                     /locus_tag="ACHY03_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFS3907714.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(282..806)
                     /gene="aroL"
                     /locus_tag="ACHY03_004563"
     CDS             complement(282..806)
                     /gene="aroL"
                     /locus_tag="ACHY03_004563"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFS3907715.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(989..1447)
                     /locus_tag="ACHY03_004564"
     CDS             complement(989..1447)
                     /locus_tag="ACHY03_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFS3907716.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1567..2376
                     /gene="proC"
                     /locus_tag="ACHY03_004565"
     CDS             1567..2376
                     /gene="proC"
                     /locus_tag="ACHY03_004565"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFS3907717.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRSQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(2393..3508)
                     /gene="adrA"
                     /locus_tag="ACHY03_004566"
                     /gene_synonym="dgcC"
     CDS             complement(2393..3508)
                     /gene="adrA"
                     /locus_tag="ACHY03_004566"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFS3907718.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(3610..3930)
                     /gene="psiF"
                     /locus_tag="ACHY03_004567"
     CDS             complement(3610..3930)
                     /gene="psiF"
                     /locus_tag="ACHY03_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFS3907719.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(4049..5464)
                     /gene="phoA"
                     /locus_tag="ACHY03_004568"
     CDS             complement(4049..5464)
                     /gene="phoA"
                     /locus_tag="ACHY03_004568"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFS3907720.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKSAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NTRADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKTTYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(5565..5825)
                     /gene="iraP"
                     /locus_tag="ACHY03_004569"
     CDS             complement(5565..5825)
                     /gene="iraP"
                     /locus_tag="ACHY03_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFS3907721.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1368 a         1572 c         1440 g         1491 t
ORIGIN      
        1 gtagtggcac tcagaatccc ttccgagcag tctgatgcgg tcactctttg tcagggtcct
       61 catagcgttt tttcgcatcc atcgcttcct gagcggtcgg atgttcactg atcaacgagt
      121 ctggtttagg atgatcggct ctgagttggt accaggttac cgtctgtcct ggctttcctt
      181 tttcaatcgt cactatatat gcttcacgag gataaggcgg tttcgttggc atcgttcttc
      241 cttatttcac gggatgaacg ttaagtatag gcgctcgaaa atcaacaatt gatcgtctgt
      301 gccagggcgc tgcgaatttc agaaatcacc tggctgggtt cgtttgttgc gtcgatgata
      361 atatgcgcaa cttcgcgata tagcgcatcg cgttcttcca gcacttcctg aacttcttcg
      421 ctcagcggtt ttcccgttaa ggttggccgt aaatcttctt ccggtgcagc ttgcagtcgg
      481 ttaaccagga ctgatactgg cgcacacaaa taaaccacga tcccgttatt ttgcatgaag
      541 tgacgattaa attccgtcag aataatgccg ccgcctgtag cgataacggt ggatggcgca
      601 gttaccgctt ccagcgccgc cgtttctctg gcgcgaaatc ccgcccactc ttccctttcg
      661 acgatctccg cgaccgtcat attgagctgt gattgcaacc actgatcggt atcgacaaac
      721 cgacggttaa gcgaatcggc aagggccatt ccgaccgttg ttttaccaca gccccgaggc
      781 ccaatcagaa aaagaggttg tgtcatcgtg ggttttcccc aataggtcgc aatgcggcga
      841 aagccggtgt catgagaata gcgatcatac catcaaacta gtacaatttc gattgtaaag
      901 aaaaaattcc acttaaagtg aaaatctcaa tacatccctt actataccaa taaatattca
      961 agaatgaagt gtaaataata aattacattt agccacgact acgttgcact tccagccacc
     1021 acttctccag ctccgcggca aatgcctggc ggtcacgttg tgaaaggcta tctggtccgc
     1081 cggtctggat cccactggca cgtaaggtat ccataaaatc gcgcatcgtc aggcgctcac
     1141 gaatggtcgc tggcgtgtaa cgttcaccgc gcggattaag cgccgcagcg cctttctcga
     1201 tggcttcagc agccaaaggt atatctgcgg tgatcaccaa atcgcccgct tcacactgcc
     1261 ggacaatttc gttatcggca acgtcgaaac ctgccgcgac gcgcagcgta cgaataaatc
     1321 gcgatggcgg cacgcgtaaa ctctggtttg ctaccagtac cagcggcatc tgcatacgtt
     1381 ccgccgcgcg atacaaaatc tctttaatta cattgggaca cgcgtcggca tccacccaaa
     1441 ttgtcataaa gtcatccttt gttgggtaat cctctattgt gtcgcgcttt tgccttccgg
     1501 catagttctg tttatgcttc tgccagcgat tatcaaaaca atgaatttca cggcaggagt
     1561 gaggcaatgg aaaagaaaat cggttttatt ggctgtggca atatgggaaa agccattctc
     1621 ggcggtctga ttgccagcgg tcaggtgctt ccagggcaaa tctgggtata caccccctcc
     1681 ccggataaag tcgccgccct gcatgaccag ttcggcatca acgccgcaga atcggcgcaa
     1741 gaagtggcgc aaatcgccga catcattttt gctgccgtta aacctggcat catgattaaa
     1801 gtgcttagcg aaatcacctc cagcctgaat aaagactctc tggtcgtttc tattgctgca
     1861 ggtgtcacgc tcgaccaact tgcccgcgcg ctgggccatg atcggaaaat tatccgcgcc
     1921 atgccgaata ctcccgcgct ggttaatgcc gggatgacct cggtaacgcc aaacgcgctg
     1981 gtaaccccag aagataccgc tgatgtgctg aatattttcc gctgctttgg cgaagcggaa
     2041 gtaattgctg agccgatgat ccacccggtg gtcggtgtga gcggttcttc gccagcctac
     2101 gtatttatgt ttatcgaagc gatggccgac gccgccgtgc tgggcgggat gccacgctcc
     2161 caggcgtata aatttgccgc tcaggcggta atgggttccg caaaaatggt gctggaaacg
     2221 ggagaacatc cgggggcact gaaagatatg gtctgctcac cgggaggcac caccattgaa
     2281 gcggtacgcg tactggaaga gaaaggcttc cgtgctgcag tgatcgaagc gatgacgaag
     2341 tgtatggaaa aatcagaaaa actcagcaaa tcctgatgac tttcgccgga cgtcaggccg
     2401 ccacttcggt gcggttacgt ccggctttct ttgctttgta gagcgccaaa tctgccgatt
     2461 tcaaccactc acgatagtga ctcatttgtg ggttcagcgg cgcaaccccc acactaatcc
     2521 gtaaagttac ctgtggcgta ttcggcaagc gtaatgtgtt tagcccttca tgcacccgta
     2581 acatggcggt aatggcgctc tcagctggcg taccggacat gattaccgca aactcatcgc
     2641 cgccaaaccg accaatcaca tcgctaccgc gcagggtaat ttgtaactgt cgggtaagcg
     2701 ccacaatcgc ttcatcgccc acatcatggc cccaggtatc gttgatgctc ttgaaatggt
     2761 cgatatcgat aatcagtaac gttgcatcgc gattatgccg ccgacagtta tcaaattcat
     2821 tgcgtaacat agtttcccaa tgacgtcggt tatacacgcc cgtcatgccg tcgcgggtac
     2881 tcatgacctg caacctgcgt ttatgttccg ccagtttagt cgccgtctgg tagctgaccc
     2941 agccaaacag cagaggataa atgacaataa tgggaaggga gagccaccat tccagcggcg
     3001 cactattgaa cgacacggtg atgcccgtca gctcgagggt gacaaggcag gaaaccacca
     3061 tcaacaccag acccgcgaca aacagacggg ggccgcctgc ccccatcaaa ttcagacaca
     3121 taatcatcaa catcgcggtg gaaggcagca cgtttacgcc cattacgcct acccacattc
     3181 ccgctaatac tgcatcggtt tttaagttgt aaatttcccg gctaagcgga tcgacggccc
     3241 tgctcgctat ctgccaggct aaatgcggcc agacgaacgc ccagccgacc aacaccagcc
     3301 accaccagcc cggcggcggg tgtgaaacca gcgttgaagc aatcggtaag aacatgccag
     3361 ccaggccaac cgcacggggt agtctgacgc gacgggcgaa gcgcagcccg gaccgctgat
     3421 gttcgttttg aggagttaaa ggaggttcct ccccgtgcgc cgccgctttt ttgaaaaagt
     3481 tttcatcatt cattattttt gggaacattc agaaacaatt ttcccaaatt atagagacgg
     3541 ggctaaaggc agaagtatga aatttcgggt gagccatttt agtagctcac ccgttagcac
     3601 tatcaggtat caggcggctt tcttgaggca ggcgctcata aacttattac gatcatcacc
     3661 tttcagtgat tgttgcgttg cttgattatt acattcgcgc atcttttgct gctgtggcgt
     3721 caaacttttt tcaccaggcg cagacttgct gttcttcagg caatcactca tgtaggtctt
     3781 acgagcatcc cctttcaacg cctgtgccgt cgcctgctga ttacaggagg tcatacgctg
     3841 ttgttgtggg gttaaggttc tctcggcagc gccgacggtg gttaaaaaaa ccagaccgaa
     3901 aagcaaggta accagtaatg taattttcat cgcaccatcc ctcttcatgt tttaaccatg
     3961 agcgtatgcg cccgtgatct gccattaagt ctggttgcta acagcaaaaa aaccacccgg
     4021 cagcgaaaat tcactgccgg gcgcggtttt atttcagccc cagggcggct ttcatggtgt
     4081 agaagagatc ggtctggtcg gtcagtccaa caacattggc ggcatgcggg ccatacgccg
     4141 caatacgcaa ctgactgcct gtatgttctt gtgaatcctc ttcggagttc ccgtaactca
     4201 tcaccatcac tgcgccatct ttggtattta gcgcctgggt gaggcccgga gctttggtat
     4261 ccggcgcaac aatctggctg gcgtgggcgt gatcagcggt gactatgacc agcgtgttac
     4321 catccttttt ggcgaattcc agcgcccgtt gtacggcttc atcgagatcg accgtctcgc
     4381 caatttgccc acaaggattc gcagcatgat cctgtttatc gattgacgca ccttcaactt
     4441 gcaggaaaaa gcctttctca tttttactca acaattcaat ggctttgtcg gtcatctgtg
     4501 ccagggtcgg tacactgtca ttacgttgcg gattaggcgt acaggtgact gcgggcttat
     4561 cgatattacc gtggtacgtt gttttcggtc ctagccagcg cactggcata ttgccgtcag
     4621 caaacaggcc aagcaggggt ttttgctgat tcgcttccgt caccgaattc agtgaggcag
     4681 catcgctcac caactgataa ccacgcgcct gtgcctgttc acgcagcgtt tttccctgcc
     4741 attcaccagc ggttgccgtt tcagcaaagg tttttgcgcc gccgccaagc gtaacgtcgg
     4801 cacgagtgtt aagcagctgt tcggtaatcg atccttttcc gcctttttcc agagcgttac
     4861 ccggacattt ttcactggtc gcgctcggac cgtagcattt gcgcgaggtc acatgtgcca
     4921 ccagcgcagc gggcgtggca tcctgcaact ctgcggtaga aacgttaccg gtcgccagac
     4981 ctgcggcttt tgccatttcc agaatcgttg ggtgatcttt ttcgtgaata tcgacgccca
     5041 gcgcgccgtt ataggttttg acaccggttg accaggcggt tgctgatgca gccgagtcgg
     5101 tgacgtagtc cggtttgccg gtttttttat tcagcgcata gtgagtgtat tgcccggtaa
     5161 gcggtaaggc atcgatacct ttaaaaaagc cgcccgcacc ttcggcataa ttacgtgcgg
     5221 cagtaatttc cgaatccccc atcccatcgc caatcagcaa aataatattt tttgcagatt
     5281 tatcgctaag agaatcacgc agagcggcgg tctgatcagc cgttaagcgg cgagcaccgc
     5341 cgggtgcagt aatatcgccc tgagcagccc ggttttccag aacaggcatt tctggtgtcc
     5401 gggcttttgt cacaggggta aacagtaacg gtaagagtgc cagtgcaata gtgctttgtt
     5461 tcactttatt ttctccatgt acaaatacat taaaaaataa aaacaaagcg actataagtc
     5521 tcggccgtga caactttatg acagctgttg aaaagattaa ctttttactg acgaggatgc
     5581 ttcaataact tctttacgta atcgcgcagc agctccgtat cgtcgtcagg aatgctggca
     5641 tcgggcttta cctcgtacag cgccccctct acctgatcaa tcaaccgctg ttggtcattt
     5701 tgcgccatat tgcgaagcat tgcagtgacg ataatctcca aagcttctac ctgcgcacac
     5761 agttctttcg actcttcttc tttttgggca agcttaaata acaactcagc aatgagattt
     5821 ttcatgtctg tatttcctta tccaaagtat ggagaagtta acattatgtt t
//
LOCUS       DAZUCE010000274         5839 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZUCE010000274 DAZUCE010000000
VERSION     DAZUCE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.275"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1850)
                     /locus_tag="ACHY03_004570"
     CDS             complement(<1..1850)
                     /locus_tag="ACHY03_004570"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HFS3907722.1"
                     /translation="MTTAITSLKHKYPHAPAEEQLPYWVEILLVDEQGDAVSDMPWKV
                     ESHHPGDGIIKQFTYTGRSGSDGLIRIDMPHGLELKLTLDADSLAKEMEKRPLRVGRD
                     AEMDSLIRQLAEDKGYIWHYAVIGELCKVTPSIKLESGEGWPPYHFPHGKSFKGFIIR
                     TNELERRHVIEICPFRAWELVLHHQKDYSIANGINLGVAATLAYADDNALSKSSITNF
                     FINQCQDLSRLPRLYKDKSSWNTLVRDVPYSERYSPPVFMDTSIDSSSKEDKDNQQGT
                     ISTKNDVSNVKADGDTQLYYVYNSDKIIIAWRGTESLFDAGTDIAFNPVKTESCDVNK
                     TQCAALVPAGKVHNGFWSGYLRLGRVFNNELDELLTLIKSRDLFVCGHSLGGALALIH
                     SAALKLEKPLLYTYGMPRTFTRNAIMELSDITHFRHINDNDPIPAVPMEANLDNEWYK
                     LWGLLGGTLGFFWSLGELMAYQLVAWGDCFWHHGNTVAFLTVTQSREWKEYKRNLPSP
                     AGGITIRKRLPVKAKLYLVPVLAEQEMQEAGQKQKEFQASLTKTDLTRFFPQGGNPER
                     GVNINIFEHFMTSYMPYMYNKLLELIDKAGIVEKRTFTEHLYNIDLFKMQ"
     gene            complement(1853..4384)
                     /locus_tag="ACHY03_004571"
     CDS             complement(1853..4384)
                     /locus_tag="ACHY03_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFS3907723.1"
                     /translation="MNLTDSLQNVLSGVGTLNRYRLDIPSCPSSLDVEDFSGTEGISK
                     IYRYDIIFTSTDRYPDAAWFLRKSATLIMGTGLLESLTEQKKVHGVITDFRRISGSED
                     QAQYRITLKPFISLLDKQFRTHRFFVNKSVPEVVEQILTEHGLKGWEYEFSLKRTYPK
                     REQINQYQESDLRFIQRLLAEVGIFYFFTLHPDAQTEVIHFGDAQAALIFDKTLPVNS
                     PSGMSDSGTDSVWALNVEHRVVESRVITNDYNHREAQNILMSVPADMTRGEGYDTSYG
                     EVYHYRPRHTERGDKIDPAPETANFWARLDHERFLVEQTRITGKSTDASLLPAQVLTI
                     TDSTPPVLPAPLQEPVLLTQLLFTGSRKSALQVMLSAVPYSEIVCWRPPLLTRPKITG
                     TMTARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFH
                     FPLIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMED
                     KRGEEHIKLSTEYGGKTQLNLGHNVNASRELRGEGAELRTNDWVSVRGGKGILLTADT
                     QPDVGSKMLEMDAAIEQLTQALTLARNLQAAVKGAKATPSDIDGQKKLNSALGYLKMP
                     GLLAHAPSGIGILSPQTVRLASGEQSIGLMSGRNTDISTGESFTVAATDTISLFARDA
                     GMQLFAGDGKVDIQAQGDELNLSSLRDMTVSSTENSISINAAKELILNCNGAYIKLSG
                     GNIELGCPGNILLKATNVDQTGPASLNTPPVSFPEGYGETFITTSRQSGKIKPFTTYR
                     ITTSEGEVYEGISDSEGKTSSIFTSMPSQIKIEYPRNVSENKEEE"
     gene            complement(4371..4913)
                     /locus_tag="ACHY03_004572"
     CDS             complement(4371..4913)
                     /locus_tag="ACHY03_004572"
                     /inference="COORDINATES: protein motif:HMM:NF045617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative T6SS immunity periplasmic lipoprotein"
                     /protein_id="HFS3907724.1"
                     /translation="MVKIRAFRYRSALLLCLTLLTGCPGRGDYYRFDETVTVSTRGDN
                     VCFSVPEPEDYQPVSVSIAPRGTRFRERQITSEPSLRIVNGQLCIPPSFCLFPDKGQF
                     IVRSVLYSERDKDTPRIMVAGIEIADGCIFDIPLNDMEVVRPYGELKSSNVQTDQSER
                     HGPCKYPFRAVRGVINHESH"
     gene            complement(4907..5473)
                     /locus_tag="ACHY03_004573"
     CDS             complement(4907..5473)
                     /locus_tag="ACHY03_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFS3907725.1"
                     /translation="MTGYGRNQDAGAGKNQREEGSLRLLTPGEIALARLVFRSTIPYE
                     KVWIHHDSFLPFGLQNKQTAMVPDGKIYFREHYRDDYSLSVPFYQHMFIHEMAHVWQR
                     EKGMNVIGRGMFSWAVNYRYVLDGRLLSEYPMEQQAQIIADHFTLQAEGYGTWCDMRR
                     DGDITLDGNMSEYVIRSLYTSTLRGFPW"
     gene            complement(5470..>5839)
                     /locus_tag="ACHY03_004574"
     CDS             complement(5470..>5839)
                     /locus_tag="ACHY03_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFS3907726.1"
                     /translation="LRDHFQPALLARFQTVIYRPLTQSAMRTIVEMKLAQVCERLHCH
                     YGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSHMAAKQKPQAL
                     ALAWSDEDGMVIELRQECAL"
BASE COUNT         1510 a         1499 c         1232 g         1598 t
ORIGIN      
        1 atttgcattt tgaacaagtc aatgttataa agatgttcgg tgaatgtacg tttttcaact
       61 atcccagctt tatcaattaa ttcaagcaac ttattgtaca tataaggcat atatgacgtc
      121 ataaagtgct caaaaatatt tatgttcact cctcgctcag gattaccacc ttgtggaaaa
      181 aaacgagtca aatctgtttt tgttagtgac gcctgaaatt ctttctgttt ctgacctgcc
      241 tcctgcattt cctgttctgc caatacaggt actaagtaaa gcttagcttt aacggggagt
      301 cgttttctga tagtaatccc ccctgcaggg cttggcaggt ttcttttata ttctttccat
      361 tcccgactct gagtgaccgt aagaaaagct actgtattac cgtgatgcca aaaacagtct
      421 ccccaggcca ccaactgata agccatgagt tcacccaatg accaaaagaa acccagggta
      481 ccaccaagca atccccaaag tttgtaccac tcattatcca gattagcttc catgggaact
      541 gctggaatcg gatcattatc attaatatgt cgaaaatgtg taatatccga taactccatt
      601 atggcatttc gagtaaaggt ccgaggcatt ccgtaagtat aaagcagagg tttctctaat
      661 tttaatgctg cagaatgaat taatgccaga gctcccccta aactatgtcc acaaacaaac
      721 aaatcccttg atttaattaa cgttaaaagc tcatcaagtt cattattaaa tactcttcca
      781 agtctgagat atccactcca aaacccatta tgcacttttc ctgcaggcac taatgctgca
      841 cattgtgttt tatttacatc acatgactct gtttttactg gattgaatgc aatgtctgta
      901 cccgcatcaa acaaactttc tgtacctcgc caagcaataa ttatcttatc tgaattataa
      961 acataataaa gctgcgtatc accatccgcc tttacatttg aaacatcatt cttagtagaa
     1021 atagttccct gctgattatc tttatcctct ttagatgagg aatctattga cgtatccata
     1081 aatacgggtg gtgaataacg ttcactataa ggcacatccc ggaccagcgt attccaggat
     1141 gacttatctt tataaaggcg aggcagacgg gaaagatcct gacactggtt aataaaaaaa
     1201 ttcgtaattg aagatttact taaagcatta tcatccgcat aagcgagtgt tgctgcaacg
     1261 ccaagattaa taccatttgc aatggaataa tctttttgat ggtgcagaac aagttcccat
     1321 gccctgaaag ggcatatttc aataacatgt cgcctttcaa gctcatttgt tctgataata
     1381 aatcccttaa atgacttgcc gtgaggaaaa tgataaggag gccagccctc cccactttcg
     1441 agcttaattg atggagtaac cttacaaagc tcaccaataa cagcataatg ccagatatat
     1501 cctttatctt ctgctaattg ccgaatgagt gaatccattt ctgcatccct gccaacccga
     1561 agagggcgtt tctccatctc ttttgccagt gaatcagcgt ccagtgtaag tttcaactca
     1621 agaccatgtg gcatatcaat tcttataaga ccatccgaac cgcttcgccc ggtatatgta
     1681 aactgcttaa ttattccatc accagggtgg tgactctcca ctttccatgg catgtctgat
     1741 acagcatccc cttgttcatc cacaagcagt atttcaaccc aatagggtaa ttgttcttct
     1801 gccggagcat gaggatattt gtgtttaaga gaagtaattg cagtagtcat gattattctt
     1861 cttccttgtt ctctgataca ttacgcgggt attcaatctt tatctgagag ggcattgatg
     1921 taaaaattga tgatgtttta ccttctgaat cagaaatacc ctcatacact tccccttcac
     1981 ttgttgtgat cctgtatgtt gtaaaaggtt taatttttcc actctgacgt gaagtcgtaa
     2041 tgaacgtttc tccatatcct tcaggaaatg aaaccggagg cgtatttaaa gatgcaggac
     2101 ctgtttgatc tacatttgta gccttgagca gaatgttacc gggacatccc agttcaatat
     2161 tgcctccact caatttgata tatgcaccat tacaattcaa tataagttct ttagcagcat
     2221 taattgaaat actgttctcg gtactgctga ccgtcatatc cctgagggat gaaagattga
     2281 gctcatcccc ctgagcctgt atatctactt taccgtcccc cgcgaatagc tgcataccag
     2341 catcacgggc aaataaactt atcgtatctg tagccgcaac agtgaaggat tcgcctgtac
     2401 tgatatctgt atttcttccg gacatcagac cgatactttg ttctcctgac gccagacgaa
     2461 cggtctgtgg gctaagaata cctattccag aaggtgcatg tgccagtaat cctggcattt
     2521 tcaggtatcc cagcgcacta ttaagcttct tctgaccgtc aatatccgat ggcgtagctt
     2581 ttgctccttt tactgccgcc tgcaaatttc gggcaagtgt cagagcttgg gtcaattgct
     2641 caattgccgc atccatttcc agcattttac ttccgacatc aggctgagta tctgcggtca
     2701 gtaatatccc cttccctccc cgcacgctga cccagtcgtt tgtacgcagc tctgcacctt
     2761 ccccccgaag ttcccgggag gcattaacgt tatggccaag attaagttgt gtcttaccgc
     2821 catactcggt actgagcttg atatgctcct caccgcgttt gtcctccatc cgcaacttgt
     2881 tatttgccgg cgtacggata acattgcgcg taccgttctt atcggtaacg tgatcaacat
     2941 gacgtgaatc atgcagggca tgcgctatat agggacgatc cggatcacct tcctcaaaag
     3001 caatggccac ttccgttccc tgaatcagcg gaaaatgaaa accataagca tcaccactgt
     3061 agggtttggc cagacgtacc ggcatacttt cctgtcccgg atttttatca tcacggtcag
     3121 catcaaattt cacccggtac atgcctgaag catcctgcca ggcgtagata tccccttctt
     3181 ttgcactggt cacacgggcc gtcatggtcc cggtgatttt cggacgagta agtaacggtg
     3241 gtcgccagca gacaatctcg ctgtaaggaa cagcactcag catcacctgt agcgcagatt
     3301 tccggctacc ggtgaacaga agctgcgtga gcagaaccgg ctcctgtaac ggtgcaggca
     3361 acactggtgg agtactgtca gtaatagtca gcacctgtgc cggtaacaga ctggcatcgg
     3421 tgcttttacc tgtgatacgt gtttgctcaa ccagaaaacg ctcgtgatcg agccgggccc
     3481 agaagttagc cgtctccggc gcagggtcaa ttttatcgcc cctttctgta tggcgtggcc
     3541 ggtaatgata aacctcgcca taactggtgt cgtaaccttc tccacgggtc atgtccgccg
     3601 gtacggacat cagtatgttc tgggcctcgc ggtggttata gtcgttcgtg ataacacggc
     3661 tttccaccac ccggtgctcc acattcaatg cccatactga gtctgtgcca ctgtcactca
     3721 taccggacgg gctgttaacg ggcagtgttt tatcaaatat caacgccgcc tgggcgtcac
     3781 cgaagtggat cacctctgtc tgcgcatccg ggtgcaacgt aaagaagtaa aaaatgccca
     3841 cttctgccag caggcgctgg ataaatctca gatcgctttc ctgatactgg ttaatctgtt
     3901 cgcgcttcgg atacgtcctt ttgaggctga actcatactc ccagcctttc agcccgtgtt
     3961 ccgtcaggat ttgctccacc acctcaggca ctgatttatt aacgaaaaaa cggtgtgtac
     4021 ggaactgttt atccagcagg gaaatgaaag gtttaagtgt gatccggtat tgtgcctggt
     4081 cctcagagcc agaaatacgt ctgaaatcag taataacacc gtgcactttt ttctgttcag
     4141 tcaggctttc cagtaatccg gttcccatta tcagggttgc ggatttacgc aggaaccagg
     4201 cagcatcagg atacctgtcc gtactggtaa aaatgatgtc ataccggtaa atcttactta
     4261 tgccttcagt tccgctgaaa tcttcaacat ccagcgatga cgggcaggaa ggtatatcca
     4321 gcctgtagcg gttcagggtt cccaccccgg ataaaacatt ttgcagggag tcagtgagat
     4381 tcatgattta tcactccacg aacagctctg aaagggtatt tgcagggacc atgacgttcg
     4441 ctctggtcag tctgaacatt actgcttttc agctcaccgt atggcctcac gacctccatg
     4501 tcattaagcg ggatatcgaa aatacacccg tcggcaatct ctatgccggc aaccatgatg
     4561 cgcggtgtgt ccttatcgcg ctcagagtac agaacagacc tgacaatgaa ctggccttta
     4621 tcaggaaaaa gacagaatga aggcggaatg cacagctggc cgttcacgat acgaagcgag
     4681 ggttcagatg taatttgccg ctccctgaat cgggttcccc gtggagcgat ggaaacgctg
     4741 actggctggt agtcctctgg ctccgggaca ctgaaacaca cattgtcgcc tcttgtgctg
     4801 accgttactg tttcatcaaa ccgataataa tccccgcgtc cggggcatcc tgtcagtaac
     4861 gtcagacaga gcagtaaagc agaacgataa cgaaaggctc ttatttttac catgggaacc
     4921 cccgcaacgt gctggtatac aggctgcgaa taacatactc agacatattt ccgtccagtg
     4981 tgatatcacc gtcccttctc atatcacacc atgtcccgta tccttcagcc tgcaaagtaa
     5041 aatgatcagc aataatttgt gcctgctgct ccatgggata ttcgcttaac agacgcccgt
     5101 ccagaacata acggtaattc accgcccaac tgaacatccc ccgaccgatg acattcattc
     5161 ccttttcacg ctgccagaca tgcgccattt catgaataaa catatgctga taaaagggaa
     5221 cgctgagtga ataatcatcc cgatagtgct cacggaaata aatcttacca tcggggacca
     5281 tggcagtttg cttattctga agaccaaaag gcagaaaact gtcatggtgg atccagacct
     5341 tttcataagg gattgtcgac ctgaatacca gcctcgccag cgcaatctct cccggcgtca
     5401 gtaatcgcaa tgagccttct tccctttgat ttttacctgc gccagcgtcc tgatttctgc
     5461 catagcctgt cataacgcgc attcctgccg cagctcaatc accataccgt cttcgtcact
     5521 ccatgccaga gccagcgcct gtggtttctg cttcgcggcc atatggctca gcaactgccg
     5581 gctcagtacc ggcagcagct gctggttcag cagactctcc acattccggg cccccgtatc
     5641 cggcagcagg caggctgatg tcagggcatc gtacacacgt tcatcaaccg atgtgctcag
     5701 tccataatgg cagtgcaggc gttcgcagac ctgtgcaagc ttcatctcca caatggtgcg
     5761 catggcagac tgcgtcagcg gacggtaaat cacggtctga aagcgagcca gcagggcagg
     5821 ctggaagtgg tcgcgcagc
//
LOCUS       DAZUCE010000275         5759 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZUCE010000275 DAZUCE010000000
VERSION     DAZUCE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.276"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..400
                     /locus_tag="ACHY03_004575"
     CDS             <1..400
                     /locus_tag="ACHY03_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFS3907727.1"
                     /translation="LRDHFQPALLARFRTVIYRPLTQSAMRIIVEMKLAQVCERLHCH
                     YGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYLAARQHPTYL
                     SLTWHDEDGIVIAFDGINSDYPQESIYGQC"
     gene            387..1028
                     /locus_tag="ACHY03_004576"
     CDS             387..1028
                     /locus_tag="ACHY03_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907728.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTGHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSRAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            1025..1252
                     /locus_tag="ACHY03_004577"
     CDS             1025..1252
                     /locus_tag="ACHY03_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907729.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            1331..3850
                     /locus_tag="ACHY03_004578"
     CDS             1331..3850
                     /locus_tag="ACHY03_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFS3907730.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEIVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKITGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            3847..5529
                     /gene="tle1"
                     /locus_tag="ACHY03_004579"
     CDS             3847..5529
                     /gene="tle1"
                     /locus_tag="ACHY03_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFS3907731.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNSISNGSYRGYYSNI
                     HWLNSLYVADQTLLESQVEAQIQTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSVV
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PEFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFEPIRQTNTELQLPSE
                     LIFLADKAIAQGKAVRLGQEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            5549..>5759
                     /gene="tli1"
                     /locus_tag="ACHY03_004580"
     CDS             5549..>5759
                     /gene="tli1"
                     /locus_tag="ACHY03_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFS3907732.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYK"
BASE COUNT         1557 a         1148 c         1530 g         1524 t
ORIGIN      
        1 gctgcgcgac cacttccagc cagcactgct tgcccgtttc cggaccgtga tttaccgtcc
       61 gctgacgcag tctgccatgc gcatcattgt ggagatgaag cttgcacagg tctgtgaacg
      121 cctgcactgc cattatgggc tgagcacatc ggttgatgaa cgtgtgtacg atgccctgac
      181 atccgcctgc ctgctgccgg atacgggcgc ccggaatgtg gagagtctgc tgaaccagca
      241 gctgctgccg gtactgagcc ggcagttgct cagctatctt gctgccagac agcatccgac
      301 gtacctgtct ctgacgtggc atgatgagga tggtattgtg atagcgtttg atggaatcaa
      361 tagcgattat ccgcaggaaa gcatatatgg acagtgctaa agaaattgcc cgggatatgc
      421 tggaggccgt aaatattaat ggggatgggt ttctgggggc cgtcgggaag ggacttatat
      481 ctttgccagt cagtctcggt tatatgggat atgattttat agataccggg cataggcggg
      541 aaaaccagaa tgacaaattc agaatggcca gactggtaga aaaaggcatt ttcagccgtg
      601 atattattgg gaaaattatc agtatttgtc ttgaagactt tgtatcccgg atcaatatgg
      661 aaaaaacagg ctccattgtg aagaacgttt caggagatct ggcaggtaga atggcatttg
      721 ctgaattgac cggggttaaa ctgggagagg ttctggtatc cagagggctg gctgcttttt
      781 ttgcagggag ttttgccggg ttgttattat ctgttggcgc agagacatcg cgtgcgattt
      841 acacatcccg ttttctgaaa gacagaaatc cggtgttgca tgataagctg aaagctcagg
      901 gggatttgga tcttttgtac tttcttgttg aagatattgt gcgcccgttt gaacgagcct
      961 gtattgtcgc agaccgaaat tcggctgagt ttgaccggat atgcgaatat tttttcggtg
     1021 gtttatgaag ataaaaagaa cattacttat aatgctttcc cgatttatcc gtggcgcagg
     1081 tatggggtta ggtgcgtcgg gtattgtatt tacagtctgg ttcttttttc tctcatctgc
     1141 tgaatttaaa tatctgtggg gagggctctc tgttgcgtta ttttttgtcg gttatctgat
     1201 gtatcgattt gcatatgcat atatttacga tgaatgggat gattatcagt aattctctct
     1261 gaaaataaag cttaacggcc atcattatat aattgcagtt caataccggt acagacatgg
     1321 agttaacaat atgagtctga cagatacact ttcaaaaact atctctgctt ccctgaatcg
     1381 ttaccattta attattccct cctgcccgtc accactggat gttgagcatt tcagcggcag
     1441 ggaggagatg agtcagacct attattacat catcaatttt accagtactg acaacgacct
     1501 ggatgccggg ctgctgctgc gtaaatcagc cacatttaca atgggtacgg gagggcttaa
     1561 ggagcaggtg agccagaaag tagtacatgg tgtcattaca gattttcgtc gtatatcggg
     1621 ctccgccgac caggcaaaat accaggtgat actggaacca tttattaaac tactggacag
     1681 gcagcatcgt tctcaccgat ttttcgttaa caaatcggtg ccggaggtgg tgacagaggt
     1741 tctccaggag cacggcctga aaggctggga gtttgagttc cgtctcaaaa aaacgtatcc
     1801 gaagcgtgaa caaattaacc agtatcagga aagtgacctg caatttattc agcgtttgct
     1861 ggctgaagtg gggatttttt attttttcac cctgcaaccg gacacgcaga cggaaatagt
     1921 gcattttggt gacagccagg ctgcgctgac gtttgataag acgctggcga taaacagtcc
     1981 gtccggaatg aatgataaca gagcggattc agtgtggggg ctgaatgtga cgcacaatgt
     2041 ggcagaggcc agtgtcacca caaaggctta taaccatcgt gaagcacaat atctgttgca
     2101 gtccgccccg gcagacatga cgcatggtga tggtgacgga attaactatg gtgaggtgta
     2161 ccactaccat ttccgtcatc tggaacgggg ggataaaatc gacccggtgc cggagacggc
     2221 caatttttat gcccgccttg gtcatgaacg ttatctggcg gagcaggtac ggataaccgg
     2281 caacagcaca gacgccacgc tggcgcctgc tcaggttctg actatcaccg acagtcttcc
     2341 gccgacgttg cctgcgctgc tgcgcaatcc ggtgttgctt acctgtgtcg gtttttctgc
     2401 aagtcgcaag gatgccctgc aggttgtact gaagggcgtt ccttacagtg aagttatctg
     2461 ctggcgacca ccgttacttc ctcgtccgaa aatcaccggt accatgacgg cccgtgtgac
     2521 cagtgcaaaa gaaggggata tctacgcctg gcaggatgca tcaggcatgt accgggtgaa
     2581 atttgatgct gaccgtgatg ataaaaatcc gggacaggaa agcatgccgg tacgtctggc
     2641 caaaccctac agtggtgatg cttatggttt tcattttccg ctgattcagg gaacggaagt
     2701 ggccattgcc tttgaggaag gtgatccgga tcgcccctat atcgcgcatg ccctgcatga
     2761 ttcacgtcat gttgatcacg ttaccgataa gaacggtacg cgcaatgtta tccgtacgcc
     2821 ggcaaataac aagttgcgga tggaggacaa acgcggtgag gagcatatca agatcagtac
     2881 cgagtttggc ggtaagacac aacttaatct tggtcataac gttaatgcct cccggaaact
     2941 caggggagaa ggcgctgagc tgaggactga tgactggata agtatccggg gaggaaaagg
     3001 aatatttatc agtgcagata tgcagcctca ggcacaagga aagatgctgg atatgaatga
     3061 agctatccgc caactggagc aggcattatc tctggcccga agtatggcga aggccgcaac
     3121 tgctgcaaat gctactcagg gagatataag ttgtcagcaa cggttgaatt catcgctaac
     3181 agatctcact gcgccgggaa tgctgttgca tgctcctgat gggattggca tggttagtgc
     3241 acgggcgtta cgaattgctt cgggtagtga aagcgtgggc ataatgtcgg gagataacac
     3301 tgatattagt gccgggcagt catttacagt tgtagcagaa ggtgctgtta gcctgttgag
     3361 cagaaaccag gggatgcaac tgttagcagc aaaagggcgg gtaaatattc aggctcaaag
     3421 tgacgatttg tcaatgagtt cccaacagaa tcttgatatt caaagttctg agggcaaagt
     3481 gactgtttct gctaatcagg aactgatact ggcatgtggt ggagcttata tcaaactaag
     3541 cggtggaaat attgagctgg gatgcccggg acagatcctg ttaaaaagca cgaatatgca
     3601 gaagatggga ccaacaagtc ttgatattgc gtctgttgaa atgccgcgag ggtttggtgg
     3661 cggttttatt cttaccgatg aagcgggagt gccgcaacct tcgacgcctt atcgattaac
     3721 aacggctgaa ggtgatattc tacaagggat caccgatgaa aatggtaaaa cagcgccagt
     3781 caacacctcc attccgtcag tagtaaaagt tgagtttggg aaggtaaaaa ttcatggaga
     3841 aacagaatga caaaatatca gggatatgac gtaacagatg ccacccataa aaccagtatt
     3901 cataatgact ggaaagttgt tgttgcaaag aaaaaaccgg cgcggggtgt gactttaacc
     3961 attggtatat ttttcgatgg taccggaaac aatcgggaga atacagcatc tcgcctgatg
     4021 aaatttaatg agtgtagtgc agccagacag ggggtaaatc aaaaagatgc acaaagctgt
     4081 gaggactttc ttgaagaaat taacaagaac agtatctcga atggcagtta tcgaggatat
     4141 tattccaata ttcactggct aaattcactt tacgtagcag atcagacttt gttggaaagt
     4201 caggttgaag cacaaattca gacttacatt tccgggatag ggactgcggc tggtgaagca
     4261 gattcagtca ttggcatggg attaggcacc agtattctgg atatatttga aggtgtggtg
     4321 acgaaaaccg acgaggcaat ggaacgaatc acccaggcgt tatcagaatt tatggggttc
     4381 aatttaaatc ctgatttttg tatcgcaaaa atccagtttg atgtttttgg ttttagccgg
     4441 ggagcagcgg cggcaaggca ctttgccaat cgggttatgg aacaggatcc tgctatagcc
     4501 agagctattg ctaaagggct cagaggcgat ttttatgatg gtaaaccttc tggtgaagtc
     4561 cgttttctgg ggctttttga tactgtcgca gctattggtg gaatcagtaa tttcttcgat
     4621 attaatggac gtagcaaccc gggagtaaag ctggaattgc gtccttcagt cgtgaagaaa
     4681 gttttccaga ttactgcaat gaatgaatac cgatataatt tcagcctgaa cagtattaaa
     4741 ggtatgtggc ccgaactggc tttaccaggg gcgcattcag atattggagg tggttacaat
     4801 ccggtaggtt cacctctgca ggaaaatgaa agtctgtttc tgagttgccc ggaatttgaa
     4861 attgtcagtg acgataccag agaaacggat acgcgagtat accgtaatgc agagcaagcc
     4921 agaaaaatgt tgatgactct gccagcctta aaacatattt tgcctcatgg aaaactaacc
     4981 accaaaattc gttccgtagg tgtgaacaac agcaatcaac gtcgggctgg tgtgatacaa
     5041 aagcaggtgg gagccgcagt attttttgag cgtatggcag tacctaatga ctgggctaat
     5101 gtttgcctgc gtgtgatgct ggatgctgct caggaagcag gggtattgtt tgaaccaatt
     5161 cgacaaacaa atactgagtt acaacttcca tctgaactaa tatttcttgc tgacaaagcc
     5221 attgctcagg gaaaagcggt acgtttgggg caagagcctc aggcttttac tgaggaagag
     5281 ttgtatatta tcggaaaata cacccattgt tctgccaact ggaatattga gtctgatggt
     5341 aatttatggg tggatcctac aacaggagag atttttatcc atcgctttgg tcctaaaggt
     5401 aataaggcgt tcgtttttcc gaataaacca aatgatcgct ggattcgttc tgtatggtac
     5461 atggatgatc agcagcgatt gaatgataac gcagtaaaaa atactaaagt tatgatgtct
     5521 ggtgtttaac accacagggt ggcaatatat gaccagagat aaaatcattt ttatcgcacc
     5581 attactggca ttgttaactg ggtgtaattc ggttagctca cagtatgctg attcggataa
     5641 gactgaatgg ggttataaaa aattacccta tgatgaatgg aaatttctct ttatttatcc
     5701 aaaggctctg cctgcgctgg ccactcaggc attgatggtg gacggggaag gttataaga
//
LOCUS       DAZUCE010000276         5743 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZUCE010000276 DAZUCE010000000
VERSION     DAZUCE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.277"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            43..585
                     /gene="fimA"
                     /locus_tag="ACHY03_004581"
     CDS             43..585
                     /gene="fimA"
                     /locus_tag="ACHY03_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFS3907733.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            805..1497
                     /gene="fimC"
                     /locus_tag="ACHY03_004582"
     CDS             805..1497
                     /gene="fimC"
                     /locus_tag="ACHY03_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFS3907734.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPVQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1528..4137
                     /gene="sfmD"
                     /locus_tag="ACHY03_004583"
     CDS             1528..4137
                     /gene="sfmD"
                     /locus_tag="ACHY03_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFS3907735.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLISSAFPELAQQQNNKCIDLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNEHIVGLNVSVPFNVLTKRRYTRENALDRAYASLNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4150..5157
                     /gene="fimH"
                     /locus_tag="ACHY03_004584"
     CDS             4150..5157
                     /gene="fimH"
                     /locus_tag="ACHY03_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFS3907736.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5168..5683
                     /gene="sfmF"
                     /locus_tag="ACHY03_004585"
     CDS             5168..5683
                     /gene="sfmF"
                     /locus_tag="ACHY03_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFS3907737.1"
                     /translation="MRRVFFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADKDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDANLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVA
                     KADAIFMINYN"
BASE COUNT         1509 a         1227 c         1386 g         1621 t
ORIGIN      
        1 tatacggatg tggagtcgat aaatgagatt gaaggaatat atatgaaatt aagatttatt
       61 tcgtctgcgc tggctgccgc actattcgcc gctacgggta gttatgctgc cgttgtagat
      121 ggcggtacaa ttcactttga aggcgaactg gtgaatgctg cctgttcagt gaatactgac
      181 tcggcagacc aggttgtcac actcggtcaa tatcgtaccg atattttcaa tgctgttggt
      241 aatacctctg cattaattcc attcactata cagttgaacg actgcgatcc tgttgttgcc
      301 gctaatgctg ccgttgcatt ttctggtcag gctgatgcaa tcaatgataa tttattggcc
      361 attgcatcca gtaccaatac aacaacagca acaggtgtcg gtattgaaat acttgataat
      421 acatccgcaa ttctcaaacc tgatgggaat agcttctcaa ccaaccagaa cttgatcccc
      481 gggaccaacg ttcttcattt ttctgcacgt tataaaggca ccggtacaag tgcatcagca
      541 gggcaagcaa atgctgacgc gacttttatt atgagatacg aataatcaaa accacgttgt
      601 tttgaattat atatcacgtc ttataacaaa gtaatgtacc ggttgtctga agcggtatgg
      661 tggcaatgta aatcgaaatc atgttcactt tgtatcatgc cgctttatta aatgaaaagg
      721 gaatgatgtg ttgtaataaa ccaaagcaat catttcttta tatttcttat ttttgccgtc
      781 aggaatacac aaggcgtatt aactatgatg actaaaataa agttattgat gctcattata
      841 ttttatttaa tcatttcggc cagcgcccat gctgccggag ggatcgcatt aggtgccacg
      901 cgtattattt atcccgctga tgctaaacag actgcggtat ggattagaaa tagccatacc
      961 aatgagcgtt ttctggtcaa ttcgtggatt gaaaacagca gcggtgtaaa agaaaagtca
     1021 ttcatcatta caccgccact atttgttagt gaacccaaaa gcgaaaatac tttgcgtatt
     1081 atttacaccg gtccaccgct ggcggcagat cgtgagtctc tgttctggat gaatgttaag
     1141 acgatccctt cggtagataa aaatgcattg aacggcagga atgttttgca actggcgatt
     1201 ttatcgcgca tgaagttatt tctccgtcca gttcaattac aagaattacc cgcagaagcg
     1261 ccggacacac tcaagttttc gcgatccggt aactatatca atgttcataa tccatcacct
     1321 ttttatgtca ccctggttaa cttacaagtg ggcagccaaa agttggggaa tgctatggct
     1381 gcacccagag ttaattcaca aattccctta ccctcaggag tgcagggaaa actgaaattt
     1441 cagaccgtta atgattatgg ttcagtaacc ccggtcagag aagtgaactt aaactaaccg
     1501 aatcatctga caatatcaga gctaattatg aaaataccca ctactacgga tattccgcag
     1561 aggtatacct ggtgtctggc cggaatttgt tattcatctc ttgccatttt accctccttt
     1621 ttaagctatg cggaaagtta tttcaacccg gcatttttat tagagaatgg cacatccgtt
     1681 gctgatttat cgcgctttga gagaggtaat catcaacctg cgggcgtgta tcgggtggat
     1741 ctctggcgta atgatgagtt cattggttca caggatatcg tatttgaatc gacaacagaa
     1801 aatacaggtg ataaatcagg tgggttaatg ccctgtttta accaggtact ccttgaacga
     1861 attggcctta ttagcagtgc atttcccgag ttagctcagc agcaaaataa taaatgtatc
     1921 gatttactga aagctgtacc tgatgccaca attaactttg attttgcagc gatgcgcctg
     1981 aacatcacta ttcctcagat agcgttgttg agtagcgctc acggttacat tccgcctgaa
     2041 gagtgggatg aaggtattcc tgctttactc ctgaattata atttcactgg taacagaggt
     2101 aatggtaacg atagctattt ttttagtgaa ctcagcggga ttaatattgg cccgtggcgt
     2161 ttacgcaaca atggttcctg gaactatttt cgcggaaatg gatatcattc agagcagtgg
     2221 aataatattg gcacctgggt acagcgtgcc attattccgc tgaaaagtga actggtaatg
     2281 ggagacggca atacaggaag tgatattttc gatggtgttg gatttcgtgg tgtacggctt
     2341 tattcttccg ataatatgta tcctgatagc cagcaagggt ttgccccaac agtacgtggg
     2401 attgcccgta cggcggctca gctaacgatt cggcaaaatg gttttattat ctatcaaagc
     2461 tatgtttccc ccggcgcttt tgaaattact gatttgcacc cgacatcttc aaatggcgat
     2521 ctggacgtca ccatcgacga gcgcgatggt aatcagcaga attacacaat tccgtattca
     2581 acagtgccaa ttttacaacg cgaagggcgt ttcaaatttg acctgacggc gggcgatttt
     2641 cgtagcggta atagtcagca atcatcgcct ttcttttttc agggcacggc actcggcggt
     2701 ttaccacagg aatttactgc ctacggcggg acgcaattat ctgccaatta caccgccttt
     2761 ttattagggc tggggcgcaa cctcgggaac tggggcgcag tgtcgctgga tgtgacccat
     2821 gcgcgcagtc agttagccga cgacagtcgt catgaggggg attccattcg cttcctctat
     2881 gcgaaatcga tgaacacctt cggcaccaat tttcagttaa tgggttaccg ctattcgaca
     2941 caaggttttt atacccttga tgatgttgcg tatcgtcgaa tggaggggta cgaatatgat
     3001 tacgattacg acggtgagca tcgcgatgaa ccgataatcg tgaattacca caatttacgc
     3061 tttagccgta aagaccgttt gcagttaaat atttcacaat cacttaatga ctttggctcg
     3121 ctttatatct ctggtaccca tcaaaaatac tggaatactt cggattcaga tacgtggtat
     3181 caggtggggt ataccagcag ctgggttggc atcagctatt cgctctcatt ttcgtggaat
     3241 gaatctgtag ggatccccga taacgaacat attgtcggac ttaatgtttc agtgcctttc
     3301 aatgttttga ccaaacgtcg ctacacccgg gaaaatgcgc tcgaccgcgc ttatgcctcc
     3361 ttgaacgcta accgtaacag caacgggcaa aatagttggc tggcaggcgt tggtgggacc
     3421 ttactggaag gccacaacct gagttatcac gtaagccagg gcgatacttc gaataatggg
     3481 tacacgggca gcgccacggc aaactggcag gccgcttacg gtacgctggg ggtcgggtat
     3541 aactacgacc gcgatcaaca tgacgttaac tggcagctgt ctggcggtgt ggtcgggcat
     3601 gaaaatggca taacgctgag ccagccttta ggggatacca atgttttgat taaagcgcct
     3661 ggcgcaggcg gtgtacgcat tgaaaatcaa actggcattt taaccgactg gcgcggctat
     3721 gcggtgatgc cgtatgccac ggtttatcgg tataaccgta tcgcgcttga taccaatacg
     3781 atggggaatt ccatcgatgt tgaaaaaaat attagcagcg ttgtgccgac gcaaggcgcg
     3841 ttggttcgtg ccaattttga tacccgcata ggcgtgcggg cgctcattac cgttacccag
     3901 ggcggaaaac cggtgccgtt tggatcactg gtacgggaaa acagtaccgg aataaccagt
     3961 atggtgggtg atgacgggca agtttattta agtggtgcgc cattgtctgg tgaattactg
     4021 gttcagtggg gagacggcgc gaactcacgc tgcattgcgc actatgtatt gccgaagcaa
     4081 agcttacagc aagccgtcac tgttatttcg gcagtttgca cacatcctgg ctcataaagg
     4141 aaattatcaa tgaagataat ctgcagatta ttattggcaa tggcatgttt gtgtctggca
     4201 aacatatcct gggctactgt ttgtgcaaat agtactggcg tagcagaaga tgaacactat
     4261 gatctctcaa atgtctttaa tagcaccaat aaccagccag ggcagattgt tgttttaccg
     4321 gaaaaatccg gctgggtagg tgtgtcagca atttgtccac ccggcacgct ggtgaattat
     4381 acataccgta gttatgtcac caactttatt gttcaggaaa ctatcgataa ttataaatat
     4441 atgcaattaa atgattatct attaggtgcg atgagtctgg ttgatagcgt gatggatatt
     4501 caatttcccc cgcaaaatta tattcgtatg ggaacagatc ctaatgtttc gcaaaacctt
     4561 ccgttcgggg tgatggattc tcgtttaata tttcgtttaa aggttattcg tccctttatt
     4621 aacatggtgg agatccccag acaggtgatg tttaccgtgt atgtgacatc aacgccttac
     4681 gatccgttgg ttacacctgt ttataccatt agttttggtg gccgggttga agtaccgcaa
     4741 aactgcgaat taaatgccgg gcagattgtt gaatttgatt ttggtgatat cggcgcatcg
     4801 ttatttagtg cggcagggcc gggtaatcga cctgctggtg tcatgccgca aaccaagagc
     4861 attgcggtca aatgtacgaa tgttgctgcg caggcttatt taacaatgcg tctggaagcc
     4921 agtgccgttt ctggtcaggc gatggtgtcg gacaatcagg atttaggttt tattgtcgcc
     4981 gatcagaacg atacgccgat cacgcctaac gatctcaata gcgttattcc tttccgtctg
     5041 gatgcagctg cggcagccaa tgtcacactt cgcgcctggc ctatcagtat taccggtcaa
     5101 aaaccgaccg aggggccgtt tagcgcgctg gggtatttac gcgtcgatta tcaatgaggt
     5161 acggagaatg agaagagtat tctttagctg tttctgcggg ttactgtgga gttccagtgg
     5221 atgggcagct gaccctttag gaacgattaa tatcaatttg cacggtaacg tggttgattt
     5281 ctcttgtacc gtaaacacag cggataagga taagacggta gatttaggca gatggcctac
     5341 gacacaatta ctgaacgctg gcgataccac ggcactcgtc ccttttagcc tgcggctgga
     5401 gggatgtcct ccgggttcag ttgcgatttt atttacggga acgccggcat ctgatgccaa
     5461 cctgctggct ctggatgatc ccgcaatggc acaaaccgtt gccatcgaat tacgtaatag
     5521 cgatcgctcc cggctcgcac tgggggaggc gagcccgact gaggaagtag atgcaaatgg
     5581 caatgtcaca ctaaactttt ttgccaatta tcgagcgtta gccagcggtg ttcagccagg
     5641 tgtggcgaaa gcggatgcga tatttatgat caattataat taatattata ttaattcgta
     5701 taatttggcg tagtcgataa gctctacaat tgaatgcaaa cct
//
LOCUS       DAZUCE010000277         5724 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZUCE010000277 DAZUCE010000000
VERSION     DAZUCE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.278"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            145..909
                     /gene="pdhR"
                     /locus_tag="ACHY03_004586"
     CDS             145..909
                     /gene="pdhR"
                     /locus_tag="ACHY03_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFS3907738.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            1070..3733
                     /gene="aceE"
                     /locus_tag="ACHY03_004587"
     CDS             1070..3733
                     /gene="aceE"
                     /locus_tag="ACHY03_004587"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFS3907739.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            3748..5640
                     /gene="aceF"
                     /locus_tag="ACHY03_004588"
     CDS             3748..5640
                     /gene="aceF"
                     /locus_tag="ACHY03_004588"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFS3907740.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
BASE COUNT         1452 a         1456 c         1580 g         1236 t
ORIGIN      
        1 tgtcaagttg ttaaaatgtg cacagtttca tgatttcaat caaaacctgt atggacataa
       61 ggtgaatact ttgttacttt agcgtcacag acatgaaatt ggtaagacca attgacttcg
      121 gcaagtggct taagacagga actcatggcc tacagcaaaa tccgccaacc aaaactctcc
      181 gatgtgattg agcagcaact ggagtttttg atcctcgaag gcactctccg cccgggcgaa
      241 aaactcccac cggaacgcga actggcaaaa cagtttgacg tctcccgtcc ctccttgcgt
      301 gaggcgattc aacgtctcga agcgaagggc ttgttgcttc gtcgccaggg tggcggcact
      361 tttgtccaga gcagcctatg gcaaagcttc agcgatccgc tggtggagct gctctccgac
      421 catcctgaat cacagtatga cttgctcgaa acacgacacg ccctggaagg tatcgccgct
      481 tattacgccg cgctgcgtag taccgatgaa gacaaggaac gcatccgtga actccaccac
      541 gccatagaat tggcgcagca gtctggcgat ctggacgcgg aatcaaacgc cgtcctccag
      601 tatcagattg ccgtcaccga agcggcccac aatgtggttc tgcttcatct gctaaggtgt
      661 atggagccga tgttggccca gaatgtccgc caaaacttcg aattgctcta ttcgcgtcgc
      721 gagatgctgc cgctggtgag tagtcaccgc acccgcatat ttgaagcgat tatggccggt
      781 aagccggaag aagcgcgcga agcatcgcat cgccatctgg cctttatcga agaaattttg
      841 ctcgacagaa gccgtgaaga gagccgccgt gagcgttctc tgcgtcgtct ggagcaacga
      901 aagaattagt gatttttctg gtaaaaatta tccagaagat gttgtaaatc aagcgcatat
      961 aaaagcgcgg caactaaacg tagaacctgt cttattgagc tttccggcga gagttcaatg
     1021 ggacaggttc cagaaaactc aacgttatta gatagataag gaataaccca tgtcagaacg
     1081 tttcccaaat gacgtggatc cgatcgaaac tcgcgactgg ctccaggcga tcgaatcggt
     1141 catccgtgaa gaaggtgttg agcgtgctca gtatctgatc gaccaactgc ttgctgaagc
     1201 ccgcaaaggc ggtgtcaacg tagccgcagg caccggtatc agcaactaca tcaacaccat
     1261 ccccgttgaa gaacaaccgg agtatccggg taatctggaa ctggaacgcc gtattcgttc
     1321 agctatccgc tggaacgcca tcatgacggt tctgcgtgcg tcgaaaaaag acctcgaact
     1381 gggcggccac atggcgtcct tccagtcttc cgcaaccatt tatgatgtgt gctttaacca
     1441 cttcttccgt gcacgcaacg agcaggatgg cggcgacctg gtttacttcc agggccacat
     1501 ctccccgggc gtttacgctc gtgctttcct ggaaggtcgt ctgactcaag agcagctgga
     1561 taacttccgt caggaagttc acggcaatgg cctctcttcc tatccgcacc cgaaactgat
     1621 gccggaattc tggcagttcc cgaccgtatc tatgggtctg ggtccgattg gtgctattta
     1681 ccaggctaaa ttcctgaaat atctggaaca ccgtggcctg aaagatacct ctaaacaaac
     1741 cgtttacgcg ttcctcggtg acggtgaaat ggacgaaccg gaatccaaag gtgcgatcac
     1801 catcgctacc cgtgaaaaac tggataacct ggtcttcgtt atcaactgta acctgcagcg
     1861 tcttgacggc ccggtcaccg gtaacggcaa gatcatcaac gaactggaag gcatcttcga
     1921 aggtgctggc tggaacgtga tcaaagtgat gtggggtagc cgttgggatg aactgctgcg
     1981 taaagatacc agcggtaaac tgatccagct gatgaacgaa accgttgacg gcgactacca
     2041 gaccttcaaa tcgaaagatg gtgcgtacgt tcgtgaacac ttcttcggta aatatcctga
     2101 aaccgcagca ctggttgcag actggactga cgagcagatc tgggcactga accgtggtgg
     2161 tcacgatccg aagaaaatct acgctgcatt caagaaagcg caggaaacca aaggcaaagc
     2221 gacggtaatc cttgctcata ccattaaagg ttacggcatg ggcgacgcgg ctgaaggtaa
     2281 aaacatcgcg caccaggtta agaaaatgaa catggacggt gtgcgtcaca tccgcgaccg
     2341 tttcaatgtg ccggtgtctg atgcagatat cgaaaaactg ccgtacatca ccttcccgga
     2401 aggttctgaa gagcatacct atctgcacgc tcagcgtcag aaactgcacg gttatctgcc
     2461 aagccgtcag ccgaacttca ccgagaagct tgagctgccg agcctgcaag acttcggcgc
     2521 gctgctggaa gagcagagca aagagatctc taccactatc gctttcgttc gtgccctgaa
     2581 cgtgatgctg aagaacaagt cgatcaaaga tcgtctggta ccgattatcg ccgacgaagc
     2641 gcgtactttc ggtatggaag gtctgttccg tcagattggt atttacagcc cgaacggtca
     2701 gcagtacacc ccgcaggacc gcgagcaggt tgcttactat aaagaagacg agaaaggtca
     2761 gattctgcag gaagggatca acgagctggg cgcaggttgt tcctggctgg cagcggcgac
     2821 ctcttacagc accaacaatc tgccgatgat cccgttctac atctattact cgatgttcgg
     2881 cttccagcgt attggcgatc tgtgctgggc ggctggcgac cagcaagcgc gtggcttcct
     2941 gatcggcggt acttccggtc gtaccaccct gaacggtgaa ggtctgcagc acgaagatgg
     3001 tcacagccac attcagtcgc tgactattcc gaactgtatc tcttacgacc cggcttacgc
     3061 ttacgaagtt gctgtcatta tgcatgacgg tctggagcgt atgtacggtg aaaaacaaga
     3121 gaacgtttac tactacatca ccacgctgaa cgaaaactac cacatgccgg caatgccgga
     3181 aggtgctgag gaaggtatcc gtaaaggtat ctacaaactc gaaaccattg aaggtagcaa
     3241 aggtaaagtt cagctgctgg gctccggttc tatcctgcgt cacgtccgtg aagcggcaga
     3301 gatcctggcg aaagattacg gcgtaggttc tgacgtttat agcgtgacct ccttcaccga
     3361 gctggcgcgt gatggtcagg attgtgaacg ctggaacatg ctgcacccgc tggaaactcc
     3421 gcgcgttccg tatatcgctc aggtgatgaa cgacgctccg gcagtggcat ctaccgacta
     3481 tatgaaactg ttcgctgagc aggtccgtac ttacgtaccg gctgacgact accgcgtact
     3541 gggtactgat ggcttcggtc gttccgacag ccgtgagaac ctgcgtcacc acttcgaagt
     3601 tgatgcttct tatgtcgtgg ttgcggcgct gggcgaactg gctaaacgtg gcgaaatcga
     3661 taagaaagtg gttgctgacg caatcgccaa attcaacatc gatgcagata aagttaaccc
     3721 gcgtctggcg taagaggtaa aagaataatg gctatcgaaa tcaaagtacc ggacatcggg
     3781 gctgatgaag ttgaaatcac cgagatcctg gtcaaagtgg gcgacaaagt tgaagccgaa
     3841 cagtcgctga tcaccgtaga aggcgacaaa gcctctatgg aagttccgtc tccgcaggcg
     3901 ggtattgtta aagagatcaa agtctctgtt ggcgataaaa cccagaccgg cgcactgatt
     3961 atgattttcg attccgccga cggtgcagca gacgctgcac ctgctcaggc agaagagaag
     4021 aaagaagcag ctccggcagc agcacccgcg gctgcggcgg caaaagacgt taacgttccg
     4081 gatatcggca gcgacgaagt tgaagtgacc gaaatcctgg tgaaagttgg cgataaagtt
     4141 gaagctgaac agtcgctgat caccgtagaa ggcgacaagg cttctatgga agttccggct
     4201 ccgtttgcag gtaccgtgaa agagatcaaa gtgaacgtgg gtgacaaagt gtctaccggc
     4261 tcgctgatta tggtcttcga agtcgcgggt gaagcaggcg cggcagctcc ggcggctaaa
     4321 caggaagcgg ctccggcagc ggcccctgca ccagcagctg gcgtgaaaga agttaacgtt
     4381 ccggatatcg gcggtgacga agttgaagtg accgaagtga tggtgaaagt gggcgacaaa
     4441 gttgccgctg aacagtcact gattaccgta gaaggcgaca aagcttctat ggaagttccg
     4501 gctccgtttg caggcgtcgt gaaggaactg aaagtcaacg ttggcgataa agtgaaaact
     4561 ggctcgctga ttatgatctt cgaagttgaa ggcgcagcgc ctgcggcagc tcctgcgaaa
     4621 caggaagcgg cagcgccggc tccggcagca aaagctgaag ccccggcagc agcaccggct
     4681 gcgaaagcgg aaggcaaatc tgaatttgct gaaaacgacg cttacgttca cgcgactccg
     4741 ctgatccgcc gtctggcacg cgagtttggc gttaacctgg cgaaagtgaa gggcactggc
     4801 cgtaaaggtc gtatcctgcg cgaagacgtt caggcttacg tgaaagaagc tatcaaacgt
     4861 gcagaagcag ctccggcggc gactggcggc ggtatcccag gcatgctgcc gtggccgaag
     4921 gtggacttca gcaagtttgg tgaaatcgaa gaagtggaac tgggccgtat ccagaaaatt
     4981 tctggtgcga acctgagccg taactgggtg atgatcccgc atgttactca cttcgacaaa
     5041 accgatatca ccgagctgga agcgttccgt aaacagcaga acgaagaagc ggcgaaacgt
     5101 aagctggatg tgaagataac cccggttgtc ttcatcatga aagccgttgc tgcagcgctt
     5161 gagcagatgc ctcgcttcaa cagttcgctg tcggaagacg gtcagcgtct gaccctgaag
     5221 aaatacatca acatcggtgt ggcggtggat accccgaacg gtctggttgt tccggtattc
     5281 aaagacgtca acaagaaagg catcatcgaa ctgtctcgcg agctgatgac tatttctaag
     5341 aaagcgcgtg acggtaagct gactgcgggc gaaatgcagg gcggttgctt caccatctcc
     5401 agcatcggcg gcctgggtac tacccacttc gcgccgattg tgaacgcgcc ggaagtggct
     5461 atcctcggcg tttccaagtc cgcgatggag ccggtgtgga atggtaaaga gttcgtgccg
     5521 cgtctgatgc tgccgatttc tctctccttc gaccaccgcg tgatcgacgg tgctgatggt
     5581 gcccgtttca ttaccatcat taacaacacg ctgtctgaca ttcgccgtct ggtgatgtaa
     5641 gtaaaagagc cggcccaacg gccggctttt ttctggtaat ctcatgaatg tattgaggtt
     5701 attagcgaat agacaaatcg gttg
//
LOCUS       DAZUCE010000278         5719 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZUCE010000278 DAZUCE010000000
VERSION     DAZUCE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.279"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            8..997
                     /gene="ldhA"
                     /locus_tag="ACHY03_004589"
     CDS             8..997
                     /gene="ldhA"
                     /locus_tag="ACHY03_004589"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFS3907741.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1108..1530
                     /gene="hslJ"
                     /locus_tag="ACHY03_004590"
     CDS             1108..1530
                     /gene="hslJ"
                     /locus_tag="ACHY03_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFS3907742.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1527..1793)
                     /gene="ydbJ"
                     /locus_tag="ACHY03_004591"
     CDS             complement(1527..1793)
                     /gene="ydbJ"
                     /locus_tag="ACHY03_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFS3907743.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2067..5591
                     /gene="nifJ"
                     /locus_tag="ACHY03_004592"
     CDS             2067..5591
                     /gene="nifJ"
                     /locus_tag="ACHY03_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFS3907744.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDTNGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1430 a         1475 c         1474 g         1340 t
ORIGIN      
        1 aagtcttatg aaactcgccg tttatagcac aaaacagtac gacaagaagt acctgcaaca
       61 ggtgaacgag tcctttggct ttgagctgga attttttgac tttctgctga cggaaaaaac
      121 cgctaaaact gccaatggct gcgaagcggt atgtattttc gtaaacgatg acggcagccg
      181 cccggtgctg gaagagctga aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg
      241 tttcaacaac gtcgaccttg acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc
      301 agcctatgat ccagaggccg ttgctgaaca cgccatcggt atgatgatga cgctgaaccg
      361 ccgtattcac cgcgcgtatc agcgtacccg tgacgccaac ttctctctag aaggtctgac
      421 tggctttact atgtatggca aaacggcagg cgttatcggt accggtaaaa tcggtgtggc
      481 gatgctgcgc attctgaaag gttttggtat gcgtctgctg gcgttcgatc cgtatccaag
      541 tgcagcggcg ctggaactcg gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc
      601 agacgttatc tctctgcact gcccgctgac accggaaaac taccatctgt tgaacgaagc
      661 cgccttcgat cagatgaaaa atggcgtgat gatcgtcaat accagtcgcg gtgcattgat
      721 tgattctcag gcagcaattg aagcgctgaa aaatcagaaa attggttcgt tgggtatgga
      781 cgtgtatgag aacgaacgcg atctgttctt tgaagataaa tccaacgacg taattcagga
      841 tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg tttaccgggc accaggcatt
      901 cctgacagca gaagctctga ccagtatttc tcagactacg ctgcaaaact taagcaatct
      961 ggaaaaaggc gaaacctgcc cgaacgaact ggtttaatct tgccgctccc ctgcattcca
     1021 ggggagctga ttcagataat ccccaatgac ctttcattct ctattcttaa aatagtcctg
     1081 agtcagaaac tgtaattgag aacaacaatg aagaaagtag ccgcgtttgt tgcgctaagc
     1141 ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg ttacgccaga acagctacag
     1201 catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg
     1261 ccagaaatca gctttggtga aaaaatgatg atttccggca gcatgtgtaa ccgctttagc
     1321 ggtgaaggca aactgtctaa tggtgaactg acagccaaag ggctggcaat gacccgtatg
     1381 atgtgcgcta acccgcagct taatgaactc gataacacca ttagcgaaat gctgaaagaa
     1441 ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta
     1501 acttataagc tggcggattt aatgaattaa tagctgccac agctcccggc ggcaagtgac
     1561 tgttcgctac agcgtttgcc gttgggtaat gcacacatcc caatcgccgt accatccagt
     1621 tgacgggcaa cagaaagcga accgccgatc attgcacaat ttgcttctcc actactggac
     1681 atcgacgctt ttaaacctgg cgctacgtgc gccgctgtcg cctgctgaac aggctcacta
     1741 ctacacgccg acaacaataa agcggcacac cctacccaaa acgctgctcg catctcttct
     1801 tcctctgatc ttcaagccaa acgacaccgc cataaataat aggcagcaca gagggcgacg
     1861 tcgagagctg tcctgcgcgt tgccccgcat ttttactttt ttatggctat ttttttgccc
     1921 tctgtttgat caaaacattc attacgctga tgtgggggac acaaaagcga aaatgcagaa
     1981 gaaagccatt tgctaaaatt gaaagattac tactgggcgc gcagcaattt cgtgcgcccc
     2041 tcatttgcgc aatgtaaggg tgtcatatga ttactattga cggtaatggc gcggttgctt
     2101 cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc tattaccccc agttccacga
     2161 tggcagaaca ggctgatgcc tgggccggaa acggcttgaa gaacgtttgg ggagacacac
     2221 cacgcgtggt tgaaatgcag tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc
     2281 agacgggtgc cctttcaaca tcgtttacgt catcgcaggg tttgctgctg atgatcccga
     2341 cgctgtacaa actggcaggc gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg
     2401 ttgccacaca tgcactctct atttttggcg atcattccga cgtcatggcg gtgcgccaga
     2461 cgggttgcgc gatgttgtgt gcggcaaacg tccaggaagc acaagacttt gctctcattt
     2521 cgcacatcgc gacgctgaaa agccgcgtgc catttattca tttctttgat ggtttccgta
     2581 cgtcccacga aatcaataaa attgtcccgc tggccgatga cactattctt gatctcatgc
     2641 cgcaggccga aattgatgct catcgcgccc gggcgctcaa cccggaacat ccggtgatcc
     2701 gcggtacgtc agccaatcct gacacttatt tccagtctcg cgaagccacc aacccatggt
     2761 acaacgcggt ctatgaccat gttgaacagg cgatgaatga tttctctgcc gcgacaggtc
     2821 gtcagtatca gccgtttgaa tattacgggc atccgcaagc ggaacgggtg attatcctga
     2881 tgggctctgc cattggcacc tgtgaagaag tggttgatga attgctaacc cgtggcgaaa
     2941 aagttggcgt gctgaaagtt cgcctgtacc gtcccttctc cgccaaacac ttgctgcaag
     3001 ctctgccggg atccgtacgc agcgtggcgg tactggacag aaccaaagaa cccggtgccc
     3061 aggcagaacc gctctatctg gatgtaatga ccgcactggc agaagccttt aataatggcg
     3121 agcgcgaaac tctgccccgt gttattggtg ggcgctatgg tctttcatcc aaagaatttg
     3181 gcccggactg tgtactggcg gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct
     3241 ttacggttgg tatttacgat gatgtgacca atctgtcact gccgttgccg gaaaacaccc
     3301 tgccaaactc ggcgaaactg gaagccttgt tttatggcct tggtagtgat ggcagcgttt
     3361 ccgcgaccaa aaacaatatc aagattatcg gtaattccac gccgtggtac gcacagggct
     3421 attttgttta cgactccaaa aaggcgggcg gcctgacggt ttctcacctt cgcgtgagcg
     3481 aacagccgat tcgttccgct tatctcattt cccaggctga ttttgttggc tgccaccaat
     3541 tgcagtttat cgataaatat cagatggctg agcgtttaaa acctggcggc attttcctgc
     3601 tcaacacgcc gtacagcgca gatgaagtgt ggtcgcgctt gccgcaggaa gttcaggccg
     3661 tgctaaacca gaaaaaagcg cgcttctatg tgattaacgc agcgaaaatc gcccgcgaat
     3721 gtggcctggc ggcccgtatt aataccgtca tgcagatggc ttttttccat ctgacgcaaa
     3781 ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc gattgccaaa agttacagta
     3841 gcaaaggcca ggatctggtg gagcgcaact ggcaggctct ggcgctggcg cgtgaatccg
     3901 tagaagaagt tccgttgcaa ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt
     3961 ccgatgccgc ccctgatttc gtgaaaaccg taaccgctgc gatgctcgcc gggcttggtg
     4021 atgccctccc cgtttcggcg ctgccgccag acggcacctg gccgatgggc actacacgct
     4081 gggaaaaacg caatatcgcc gaagagatcc ccatctggaa agaggaactc tgtacccaat
     4141 gtaaccactg tgttgccgct tgcccacact cagctattcg cgcaaaagtg gtaccgcctg
     4201 aagcgatgga aaacgcccct gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc
     4261 gcgggcagaa atatgtcttg caggtggctc cggaagattg caccggttgt aacctgtgcg
     4321 tcgaagtttg cccggcgaaa gaccgtcaga atccagagat taaagccatc aatatgatgt
     4381 ctcgcctgga acatgtcgaa gaagagaaaa ttaattacga tttcttcctc aacctgccag
     4441 aaatcgaccg tagcaaactg gaacgtattg atattcgtac atcgcagctg attacaccgc
     4501 tgtttgaata ttccggtgct tgttccggtt gtggcgagac gccgtatatt aaattgctga
     4561 ctcagctcta tggcgaccgg atgttgatcg ctaacgccac cggctgttct tcaatttatg
     4621 gcggtaacct gccctctaca ccgtatacca ccgataccaa cggtcgcggg ccggcatggg
     4681 cgaactctct atttgaagat aatgccgaat ttggccttgg tttccgcctg acggtcgatc
     4741 aacaccgtgt ccgcgtgctg cgtctgctgg atcaatttgc cgataaaatc ccggcggaat
     4801 tactgacggc gttgaaatca gacgctacgc cagaggttcg tcgagaacag gtcgcagctt
     4861 tacgccagca actcaacgat gttgccgaag cacatgaact gctgcgtgac gcagatgcac
     4921 tggtagaaaa atcaatctgg ctgattggtg gcgacggctg ggcttacgat atcggctttg
     4981 gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa cattctggtg ctggatacgc
     5041 aatgctattc caacaccggt ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta
     5101 agttcggcga gcacggcaaa cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt
     5161 acggtcatgt ttatgtggcg cagatttctc tcggcgcgca actgaaccag acggtgaaag
     5221 cgattcagga agcggaagcg tatccgggac catcgctgat catcgcttat agcccatgtg
     5281 aagagcatgg ttacgatctg gcactcagcc atgaccagat gcgccaactc acagctaccg
     5341 gcttctggcc gctatatcgc ttcgatccgc gtcgtgccga tgaaggcaaa ctgccgctgg
     5401 cgctggattc acgcccgccg tcagaagcac tggaagaaac gttacttcac gagcaacgtt
     5461 tccgtcgtct gaattcgcag cagccagaag tggcagaaca gttatggaaa gatgctgcag
     5521 ctgatttgca aaaacgctat gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca
     5581 acaccgatta aatgctctgg ataagaatta tccaattcta aaaaaaagcc cggacgactg
     5641 ttcgggcttg tctttttata tgttggaaaa tcagtggcaa tgcaatggcc caacaaaaaa
     5701 tgaaatctgt gacaataaa
//
LOCUS       DAZUCE010000279         5536 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZUCE010000279 DAZUCE010000000
VERSION     DAZUCE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.280"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1154
                     /locus_tag="ACHY03_004593"
     CDS             <1..1154
                     /locus_tag="ACHY03_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004172012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFS3907745.1"
                     /translation="DIYHRINNESEYDDSGSVEFLPLPRLNSRSERSEVQDIISVVIE
                     SGFFTSGPYIGKVEETLRLFYGAHTCIATSSGTDALKIALKSVGVEPGDEVIVPLNSF
                     AATENAVMAVGAVPVFANIDSSFNMLPDEVDRLRTSKTKAVLPVCLYGSCMYIDAIHR
                     IARKADIPVIVDAAQCFGIRSLINHCDLLALSFNPFKNIGSLGKSGAVLTLSPELARL
                     ARQYSYHGFAEGKKNIKAQNWGLNSRMDNLQAATLSVKLHHFENNAKKRCLLAARYHL
                     LLSDLSHNIILPTETHQNTWHLFPILLCKGERDSLFAFAREKGVELDIYYPVLSHCGE
                     HPLATGYSRREQFSDSEKIHSALLHLPLHNHMSLQEQNIVIEVLHDYFK"
     gene            1138..1968
                     /locus_tag="ACHY03_004594"
     CDS             1138..1968
                     /locus_tag="ACHY03_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HFS3907746.1"
                     /translation="MTILNKDWPVEFPFPTVIRTVVCSDLDETYIPSDSDKKKLSGIV
                     QLESYITSHAEKKGILAGWITGTNLDSAWRKSRGYISRSPHFICCSLGTEFYWVKNGR
                     LIPSETWAERISRSGYSRQNVNCLVKILMEKEIPLLKQPEDYQGPFKVSYYYPESPSI
                     ASDFATIQELADERRIRVVFTRCNPAAGDPSGCYDVEFIPLCCGKDQAVSFLMEETGL
                     PKEAIIAFGDSANDFAMFAQAGSGYLVGNADPFAVSQYGNRLNSAYCHGILSVLKELQ
                     "
     gene            1968..2984
                     /locus_tag="ACHY03_004595"
     CDS             1968..2984
                     /locus_tag="ACHY03_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFS3907747.1"
                     /translation="MIIGIVGCGGIARAHVNALCLNPLVTGLALYDVNPDKVRQLSEI
                     SSLPVTNCQDLEQLAGVSQGFIICTPNHLHVSVAEEVLRYKQLPFVCEKPLSTDLNSA
                     RYLQEIAPKGSIVSFNYRYNPVIDMIMRLRNERKLGELHFFSAEFNKNSALTRHHLTW
                     RDSAQQSKSSGALGDLSCHLLDLFCFIGESPVVVHGIKTVKGTRVGTKSDGQVEVDDN
                     GYVMGSSEKGAYFRVHASKSETDQNLGLHIQLVFENGEIRYSTHHENRLLLILFNDTN
                     TETIGFDALKRLPDPPRELPFWSDSFIHLHDDWCALIKYGHSSPKLADLSSGLHIQEI
                     IEAF"
     gene            2986..3255
                     /locus_tag="ACHY03_004596"
     CDS             2986..3255
                     /locus_tag="ACHY03_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HFS3907748.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            3252..4784
                     /locus_tag="ACHY03_004597"
     CDS             3252..4784
                     /locus_tag="ACHY03_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFS3907749.1"
                     /translation="MNIAIIGAGPAGIISARNAIKAGHSVVLFEKNTRIGGIWTPWSG
                     GAYRNACMQNSRYTFHYTGFPPGDIDEFPGVEQVFRYLSAVAGEDALRESTRLNTEVV
                     SLRKDAGHWVIRCASEGKDTEDIFDRVIIATGELWQPRRPPLPGEENFSGTLITSRDY
                     QEPEAFKGKNILIIGGGVSGADIASDLVPFARSVSLSVKKMGLYLPRQFPTGPNDMMH
                     SYLGRCLLSQMNYEDFIGYLDTMMPDYMQAYRASGLLPDMANNNAVHVNEKIIPNVAA
                     GLIKVKPLAERFTGEGAIEFVDASQEKYDVIITCTGYEMPDYSFIQGFDRTQLYEHFF
                     WTEDPSLAVINPPVDTAGFGAAFPYFDIISQWVMNVFSGKTSLPEKEAMRKWCAEHMA
                     SLHVKRFYDSWLETIRIGLLSGLLPDPARDFSRYWNIISSMVKPAYLATPPAFPEHGM
                     MDSLFDFRIARIRILSGLGNDALGYLLKKGDITDAEYRAALEIDPRQSISVHLPYSQT
                     YL"
     gene            complement(4817..5269)
                     /locus_tag="ACHY03_004598"
     CDS             complement(4817..5269)
                     /locus_tag="ACHY03_004598"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFS3907750.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISIILGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPVENKGIYAGNPARLIRIL"
BASE COUNT         1468 a         1343 c         1271 g         1454 t
ORIGIN      
        1 aagatatata tcaccgtatc aacaacgaga gtgaatatga tgattctggt tcagtcgaat
       61 ttctgccact tccaagactg aattcccgtt ctgaacgctc agaagtacag gacatcattt
      121 cagttgtaat tgaaagcgga ttttttacca gtggccccta tattggaaaa gtagaagaga
      181 cattaagatt attttatggt gctcatacct gtattgccac cagtagcggc accgatgcgt
      241 taaaaatagc tcttaaatct gttggagtgg aacccggaga tgaggtcatc gttccgctga
      301 actcctttgc agctacggag aatgcagtga tggccgtggg agccgttccg gtcttcgcta
      361 atattgacag ttctttcaat atgcttccgg atgaagtgga tcgcctcagg acttcaaaga
      421 caaaagccgt gttgccggtc tgcctttatg gctcatgcat gtacattgat gccattcacc
      481 ggattgcccg caaagccgac attccagtta tcgtggatgc agcacagtgc tttggtatca
      541 ggtcactcat caaccactgt gatctgctgg cgttgagctt taatccattc aagaatattg
      601 gttctcttgg taaatccggt gcagttctaa ccctttctcc tgaactggca cgcctggcaa
      661 ggcaatattc atatcacgga tttgctgaag gtaaaaagaa tattaaggcc cagaactggg
      721 gattaaacag ccgtatggat aatctgcagg ctgccacgct tagtgtcaaa cttcaccatt
      781 ttgaaaataa tgcaaaaaaa cgctgccttc ttgcagcccg ttaccatctt ttactgtctg
      841 acctgtcaca taacataata cttccgacag agacccatca gaatacatgg caccttttcc
      901 ctattctgct ctgcaaaggc gaacgtgatt ctctgttcgc ctttgctcgt gaaaagggag
      961 tggagttaga tatttactat cccgtccttt cacattgcgg agagcatcca ttggcaaccg
     1021 gatattcccg tcgagagcag ttcagtgatt ctgaaaaaat acattctgca ttacttcatc
     1081 tgccgctgca taaccatatg tcgctccagg agcaaaacat cgttatcgag gtactgcatg
     1141 actattttaa ataaagactg gcccgtcgaa ttcccgttcc cgacagtaat ccgcactgtg
     1201 gtgtgcagcg atcttgatga aacttatatt ccttcggaca gcgataaaaa aaaactgagt
     1261 gggatcgttc agctggaatc ctatattaca tcccatgccg aaaaaaaggg gattctcgct
     1321 ggctggatca caggaacaaa tctggattca gcctggcgaa aatccagggg atatatatcg
     1381 cgaagccctc actttatatg ttgcagtctc ggcaccgaat tttactgggt gaaaaatggt
     1441 cgtctcatcc cctctgaaac ctgggcagaa agaataagcc gttcaggcta cagtcgtcag
     1501 aatgtcaact gtctggttaa gattctgatg gaaaaagaga tcccccttct gaaacaacca
     1561 gaggattatc agggcccctt taaagtgagc tattactacc ctgagagccc atctattgcc
     1621 agcgattttg ccaccatcca ggaactggct gacgagcgac gaattcgtgt tgtgtttacc
     1681 cgctgtaacc cggcggctgg cgatccctct ggctgttatg acgttgaatt tatccccctc
     1741 tgttgtggca aggatcaggc cgtgtctttt ctgatggagg aaaccgggct gccgaaagaa
     1801 gccattattg cctttggtga cagtgcgaat gattttgcca tgtttgcaca ggccggaagt
     1861 ggctatctgg tgggtaatgc cgacccgttt gcagtcagcc agtacggaaa cagactgaac
     1921 agtgcttact gccacgggat actgagcgtt cttaaggagc tacagtgatg attatcggaa
     1981 ttgtcggctg tggggggatt gcccgtgccc atgtcaacgc actttgtctc aatccgctag
     2041 taactggtct ggccctgtac gatgtgaatc cagacaaagt ccgacagctc agcgaaataa
     2101 gctccctgcc ggtaacaaac tgccaggatc tggagcagct tgccggagtc agtcaagggt
     2161 tcattatctg cacaccaaac catctccacg ttagcgtggc agaagaagtg cttcgctaca
     2221 aacagctccc gtttgtgtgt gaaaaacccc tctcaacaga tctcaactct gcacgatatc
     2281 tacaggagat tgctccaaaa ggtagtatcg tgtcttttaa ctaccgatat aaccccgtca
     2341 tcgacatgat aatgcgtctg agaaatgaga ggaaattagg tgaactgcat ttcttctcag
     2401 cagaatttaa taaaaattct gcactgaccc gccatcacct gacctggcga gactccgcac
     2461 aacagagcaa aagcagcggt gctctgggcg atctatcctg tcatctgctt gatttatttt
     2521 gttttatagg ggaaagcccg gtagtggttc atgggatcaa aacggtaaag gggacacggg
     2581 tgggtacaaa atcagatggt caggttgaag tggatgataa cggctatgta atgggcagtt
     2641 cagaaaaggg agcttatttc agagttcatg ccagtaaatc ggaaacagat cagaatttgg
     2701 gactgcatat acagcttgta tttgaaaatg gtgaaatcag atattcaacc caccatgaaa
     2761 atcgcctgct cctgattcta tttaatgata cgaatacgga aacaattggc ttcgacgctc
     2821 tgaaacgctt gcctgatcca ccacgggagc ttccattctg gtctgattcg tttattcatc
     2881 ttcatgacga ctggtgtgct cttattaaat atggtcactc gtctcctaag ctggcggacc
     2941 tgtcatccgg ccttcatata caggaaataa ttgaggcatt ctgatatgga tacaattctt
     3001 ttaaccgggc tgtttgctgc atttttcaca acgtttgcgt tcgctcccca gagcataaaa
     3061 actatcagga cacgcaatac cgaaggtatc tccgttgtta tgtatatcat gttcctgaca
     3121 ggtgtaatct catggatagc gtatggcatt atgcgatctg attttgccgt actgatcgcc
     3181 aatattgtca cactgtttct ggctgcacca gtgcttgtaa taacacttat caaccgcagg
     3241 aaaaaacacg tatgaatatt gcaatcatcg gcgcgggtcc tgccggcatc atttctgccc
     3301 gtaatgcgat taaagcaggt cactccgtgg ttctgtttga gaagaatacc cgaattgggg
     3361 ggatctggac cccctggagt ggtggcgctt accgtaatgc ctgtatgcag aactcccgtt
     3421 atacatttca ttatactggc tttccccctg gcgatatcga tgaatttcca ggagtggaac
     3481 aggtattcag atatctttcg gccgtagccg gagaggatgc cctccgtgag tcgacccgac
     3541 tgaatactga agttgtttca ctcagaaaag acgccggaca ctgggtgatc cgctgcgctt
     3601 ctgaaggaaa agacacggaa gacatttttg accgggtcat cattgcaaca ggtgaactct
     3661 ggcaaccccg ccggcccccc ctgccaggtg aggaaaactt ctccggaacg ttgatcacgt
     3721 cgagagatta tcaggagcca gaggcattta aaggaaaaaa tatcctcatc attggcggcg
     3781 gtgtcagcgg tgcggatatt gcctcagacc ttgttccctt tgccagaagc gtaagtctgt
     3841 ccgtcaaaaa gatgggactt tatctgccga gacaattccc gactggcccg aatgacatga
     3901 tgcactccta tctgggcagg tgtctgctga gccaaatgaa ttatgaagat tttatcggtt
     3961 atctcgacac catgatgcct gactacatgc aggcctaccg agcatccggt ctgttgcccg
     4021 acatggcgaa caataacgcg gtacatgtta acgaaaaaat cataccgaat gtagccgccg
     4081 gtctgataaa agttaaaccc ctggcagagc gtttcaccgg agaaggcgct attgagttcg
     4141 tcgacgcatc tcaggaaaaa tatgatgtaa tcataacctg tacaggctat gaaatgcctg
     4201 attactcgtt cattcaggga tttgacagaa cacagctcta tgagcacttt ttctggacag
     4261 aggacccttc tctggccgtc attaatcccc cggtggatac cgccggtttc ggggctgcgt
     4321 ttccctattt tgatatcatc agccagtggg taatgaacgt attctccggg aagacttccc
     4381 ttccggaaaa agaagctatg cgcaaatggt gtgctgagca catggcttca cttcatgtta
     4441 agcgatttta tgacagctgg ctggaaacca tccggattgg gctgctgtca ggtcttctcc
     4501 cggacccggc ccgcgacttt tcccgatact ggaacatcat cagcagtatg gtcaaacctg
     4561 cctatctggc cactcctccc gcatttccgg agcatggtat gatggacagc ctgtttgatt
     4621 tccggatcgc cagaatacgc atactgtcag ggctgggaaa cgatgctctc ggttatttac
     4681 tgaaaaaagg tgatatcact gacgcagaat accgggcggc cctggaaatc gatccccggc
     4741 agtcgatttc tgtacacctg ccgtattcac agacctacct ctgataaaaa gggccagtaa
     4801 cggccctttt cttttttcat aatattctga tcagtcttgc cgggtttcct gcataaatgc
     4861 ctttgttctc aaccggtttt actacaactg cccccgcacc gataacagct ccgctgcata
     4921 tatacggaga cagaataatg gctccactgc caacagtaac cgaatccccc aggataatgc
     4981 tgatccagtt atccggtgac ggatctggtg ctcctgaacg aaacagatcg ttggcgaacg
     5041 tcacgttatg accaatgaag cagttctccc ccagtgttac gttctcacaa ataaacgtgt
     5101 gcgactgtat ccgacttcca ctaccaatga cacagccttt ctgtatctca acgaaggggc
     5161 cgacaaacac attgtccctg agttcacatt cataaatgtt gaccggtttg acaatacgga
     5221 cattggtccc ataaatcaca tcccggacag cacattccat aaatttcata ctcacacgtc
     5281 ctttaaatac agatgacact tgccggaatt atacagccca gattcagttg ccatgtttca
     5341 gaatagctta taagccgcac ttttaccgat actcagcaac aaatcaggtc tggatccact
     5401 tcagccggaa accggatctg cgcggcggga aacggacagg tgatatgata attaagcaac
     5461 acacataaat tcatagcaga catgatttta atcatcagta atgaaataat atctgatatt
     5521 atcagaaatc gataac
//
LOCUS       DAZUCE010000280         5522 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZUCE010000280 DAZUCE010000000
VERSION     DAZUCE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.281"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            347..961
                     /gene="evgA"
                     /locus_tag="ACHY03_004599"
     CDS             347..961
                     /gene="evgA"
                     /locus_tag="ACHY03_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFS3907751.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            966..4559
                     /gene="evgS"
                     /locus_tag="ACHY03_004600"
     CDS             966..4559
                     /gene="evgS"
                     /locus_tag="ACHY03_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFS3907752.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQNN"
     gene            complement(4615..>5522)
                     /gene="yfdE"
                     /locus_tag="ACHY03_004601"
     CDS             complement(4615..>5522)
                     /gene="yfdE"
                     /locus_tag="ACHY03_004601"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFS3907753.1"
                     /translation="DHDKSIFINMLKQADVLAENFRPGTMEKLGFSWERLQEINPRLI
                     YASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYPDAPPVRVGTSLADLCGGVYLFS
                     GIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMAYIATGKSPQRLGNRHPYMAPFD
                     VFDTQDKPITICCGNDKLFSALCQALELTELVNDPRFSSNILRVQNQAILKQYIERTL
                     KTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQARNMLIEAGGIMMPGNPIKISGC
                     ADPHVMPGAATLDQHGEQIRQEFSS"
BASE COUNT         1690 a         1192 c         1100 g         1540 t
ORIGIN      
        1 acacagtgca tgtgttgcac attatcccag gaacgttgga ggcctaattt aaatgaggtg
       61 caaaaaaaac aacagaggac taaaccgtgg cttttgcaat acaattctta cgcctgtagg
      121 attagtgaga agacttatag tgctaatgtg aaactataaa tcatcggtac aatccctgat
      181 tttattgttg acatttcata tatgccgact atttatatgg tatacttgtc gaattatctt
      241 aaaggaagct caaattttct tatttttatt gagaaaatga gatgatgcct tatgtctgta
      301 ttactacagg gagaagggag atgcttcatt gcaaagggaa taatctatga acgcaataat
      361 tattgatgac catcctcttg ctatcgcagc aattcgtaat ttattgatca aaaacgatat
      421 tgaaatctta gcagagttga ctgaaggcgg aagtgccgtt cagcgggtgg aaacacttaa
      481 gcctgatatc gtcatcattg atgtcgatat ccccggagtt aacggtatcc aggtgttaga
      541 aacgctgagg aagcgccaat atagcggaat tattattatc gtctccgcta aaaatgacca
      601 tttttacggg aaacattgtg ctgatgctgg cgctaatggt ttcgtgagta aaaaagaagg
      661 catgaacaat atcattgcgg ctattgaagc tgcaaaaaat ggctactgct atttcccctt
      721 ctctctcaac cggtttgttg gaagtttaac gtccgaccag caaaaactcg actccttatc
      781 gaaacaagaa attagtgtca tgcgctatat tcttgatggc aaggataata atgacattgc
      841 tgaaaaaatg ttcatcagca acaaaactgt cagcacttat aaaagtcgcc tgatggaaaa
      901 attagaatgt aaatcactga tggatcttta cacattcgca caacgtaaca aaatcggcta
      961 accacatgaa gtttttaccc tatatttttc ttctctgttg tggtctttgg tcgaccataa
     1021 gtttcgcaga cgaagattac atcgaatatc gtggcatcag tagtaacaac cgtgtcacac
     1081 ttgatccact acgtctgagc aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg
     1141 tgattgcagt acataagtcc caaacggcta cgttgttgca taccgattcg cagcaacggg
     1201 ttcgtggtat taatgctgac tatttaaatc ttttaaaaag agcgttaaat atcaaattaa
     1261 cactccggga atacgccgat catcaaaaag caatggacgc gctggaagaa ggtgaagtcg
     1321 atatagtgtt atcacattta gttgcttcgc cgcctcttaa tgatgacatt gctgcaacca
     1381 aaccactgat aattaccttt ccggcgctgg tcaccaccct tcacgattca atgcgaccgc
     1441 ttacctcatc aaaaccagta aatattgctc gagtagcaaa ttatccccct gacgaggtaa
     1501 ttcatcaatc atttccaaaa gcaacaatta tctcttttac aaatttatat caggcattag
     1561 catccgtctc agccggacag aatgattact ttattggtag taacatcatt accagcagta
     1621 tgatttcccg ctatttcact cactccttaa atgtagtgaa atattataac tcaccgcgtc
     1681 aatataactt cttcttaacc agaaaagaat ctgtcattct taatgaagta ctcaatcgat
     1741 ttgttgatgc tttaacaaat gaagttcgct atgaagtatc acaaaattgg cttgatacag
     1801 gaaacctggc ctttctgaac aagccattag aactcactga acatgaaaaa cagtggatta
     1861 agcagcatcc cgatttaaag gtgctggaaa atccttactc gcccccctat tctatgacgg
     1921 atgaaaatgg ctcggttcgg ggcgttatgg gggacattct taatattatt accttgcaaa
     1981 caggtttaaa tttttctccg atcaccgttt cacacaatat ccatgccgga acacagctga
     2041 atcccggcgg atgggatata ttacccggtg ctatttatag tgaagataga gaaaataatg
     2101 ttttatttgc tgaagccttc ataacaacgc cttacgtttt tgtcatgcaa aaagcgcctg
     2161 acagtgaaca aacattaaaa aaaggaatga aagttgccat tccatattat tatgagcttc
     2221 attcgcaatt aaaagagatg tatccggagg tagagtggat aaaagtcgat aacgccagcg
     2281 ctgcatttca caaggtcaag gaaggcgaac ttgatgctct ggtcgcgaca cagttaaatt
     2341 cacgttacat gatcgaccat tactatccta atgaacttta tcattttctt attcccggcg
     2401 ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga accggaactt aaggatatta
     2461 ttaataaagc actgaatgca atccccccaa gcgaagttct acgcctgacc gaaaaatgga
     2521 ttaaaatgcc caatgtgacc attgacacat gggacctcta tagcgagcaa ttttatattg
     2581 ttacgacatt atccgtttta ttagttggca gtagcctttt atggggattc tacctgttac
     2641 gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga aaaccaaata tcattccgga
     2701 aagcgctctc ggactcctta ccgaatccaa cttatgttgt aaactggcaa ggtaatgtca
     2761 ttagtcacaa tagtgcattt gaacattatt tcactgctga ttactacaaa aatgcaatgt
     2821 tgccattaga aaacagtgaa tcacccttta aagatgtttt ttctaatacg catgaagtca
     2881 cagcagagac gaaagaaaac cgaacaatat acacacaggt atttgaaatt gataatggca
     2941 tcgagaaaag atgcattaat cactggcata cattatgtaa tctgccagcc agcgaacatg
     3001 ctgtttatat ttgtggttgg caagatatta ccgagacgcg tgatttaatt catgcactcg
     3061 aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa aagtcagttt ctggcaacaa
     3121 tgagtcacga aataagaaca ccaataagct ccattatggg cttccttgaa cttctgtcgg
     3181 gttctggtct tagcaaggaa caacgggtgg aggcgatttc acttgcctac gccaccggac
     3241 aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact
     3301 atcaacttca accacaatgg gtcgatatcc ctactttagt ccagaacact tgtcactctt
     3361 tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg cagcagtaca tttcctgatc
     3421 attacctggt taagatcgac cctcaggcgt ttaagcaggt cttatcaaat ttactgagta
     3481 atgctctcaa atttaccacc gagggggcag taaaaattac gacctccctg gttcacattg
     3541 atgacaacca cgctgtaatc aaaatgacga ttatggattc tggaagtggg ttatcacagg
     3601 aagaacaaca acaactgttt aaacgttata gccaaacaag tgcaggtcgt cagcaaacag
     3661 gttctggttt aggcttaatg atctgcaaag aattaattaa aaacatgcag ggtgatttgt
     3721 cattagaaag tcatccaggc ataggaacaa catttacgat cacaatcccg gtagaaatta
     3781 cccaacaagt ggcggctgtc gaggcaaaag cagaacaacc tatcacacta cctgaaaagt
     3841 tgagcatatt aatcgcggat gatcatccga ccaacaggct attactcaaa cgccagctaa
     3901 atctattagg atatgatgtt gacgaagcca ctgatggtgt gcaagcgcta cacaaagtca
     3961 gtatgcaaca ttatgatctg cttattactg acgttaatat gccgaatatg gatggttttg
     4021 agttgactcg caaactccgt gagcaaaatt cgtccttacc catctggggg cttacagcca
     4081 acgcacaggc taacgaacgt gaaaaagggt taaattgcgg catgaactta tgtttgttca
     4141 aaccgttgac cctggatgta ctgaaaacac atttaagtca gttacaccag gttgcgcata
     4201 ttgtacctca gtatcgccac cttgatatcg aggccctgaa aaataatacg gcgaacgatc
     4261 tacaactgat gcaggagatt ctcatgactt tccagcatga aacacataaa gatttacccg
     4321 ctgcgtttca tgcactagaa gctggcgata acagaacttt ccatcagtgt attcatcgca
     4381 tccacggtgc ggctaacatc ctgaatttgc aaaagttgat taatattagc catcagttag
     4441 aaataacacc tgtttcagat gacagtaagc ctgaaattct tcagttgctg aactctgtaa
     4501 aagaacacat tgcagagctg gaccaggaga ttgctgtttt ctgtcaacaa aataactaaa
     4561 tagtggctcc cacaatgttc aaatgtggga gctatttacc agcacatctt acatttatga
     4621 tgagaactcc tggcgaattt gttccccatg ctggtcgagc gttgccgctc ccggcataac
     4681 atgcgggtcc gcgcagccgc tgatttttat cggattaccc ggcatcatta ttcctccggc
     4741 ttcaatcaac atatttctcg cctgagtttg tggcaaatta atggcctcag ccacacttaa
     4801 taacggcgct acgggcacac cgacttcatg tattttggct aaccaaactt cagctgcctg
     4861 cgtttttaac gtccgctcaa tatattgttt aagaatagcc tggttttgta cgcgtaaaat
     4921 attgctgcta aatctgggat cattaaccag ttccgtaagc tccagtgcct ggcataacgc
     4981 agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc ttatcctgag tatcgaaaac
     5041 atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt tgtggtgact tccctgtcgc
     5101 aatatatgcc atcagaccat gctccagaaa actcagcgtg gcatcaaaca tcgctatatc
     5161 gacatgcgcc cctctttggc tcttttcgcg gccataaagt gcactcacta ttccgctgaa
     5221 taaataaaca ccgccgcata gatccgcaag agaggtacca acacgcactg gcggagcatc
     5281 agggtaccct gtttccatca ttatcccgct cattgcctga atgatggtat cgtaggcagg
     5341 agcatctttt agcggaccgg tatgtccgaa acctgacgat gaagcatata tgaggcgcgg
     5401 gttgatttct tgtaaccttt cccatgaaaa ccccagtttt tccattgtac ctgggcgaaa
     5461 attctcagct aatacatcag cttgtttaag catatttata aatatacttt tatcgtgatc
     5521 at
//
LOCUS       DAZUCE010000281         5494 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZUCE010000281 DAZUCE010000000
VERSION     DAZUCE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.282"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(123..659)
                     /gene="ssb1"
                     /locus_tag="ACHY03_004602"
     CDS             complement(123..659)
                     /gene="ssb1"
                     /locus_tag="ACHY03_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFS3907754.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            914..3736
                     /gene="uvrA"
                     /locus_tag="ACHY03_004603"
     CDS             914..3736
                     /gene="uvrA"
                     /locus_tag="ACHY03_004603"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFS3907755.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3771..4127)
                     /locus_tag="ACHY03_004604"
     CDS             complement(3771..4127)
                     /locus_tag="ACHY03_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFS3907756.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4131..4547)
                     /locus_tag="ACHY03_004605"
     CDS             complement(4131..4547)
                     /locus_tag="ACHY03_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFS3907757.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4658..5371)
                     /gene="aphA"
                     /locus_tag="ACHY03_004606"
     CDS             complement(4658..5371)
                     /gene="aphA"
                     /locus_tag="ACHY03_004606"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFS3907758.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1296 a         1447 c         1458 g         1293 t
ORIGIN      
        1 atccggtata ataaattaat gagagtaata atccttgaat atttcaggag ccgaaattca
       61 ttttcgactc ctggacgcat atgtttcgcg ttgagagatc tcaacacaaa agatgcagat
      121 tcttagaacg gaatatcgtc atcaaagtcc atcggcggct cgttagacgg cgctgccgga
      181 gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact gattgccacc ctgcggctgc
      241 tgaggctgac cccaaccacc ctgcggctga ccgccaccga tattgccacc tgcaggagcg
      301 ccaccaccct gacgaccacc cagcatctgc atagtgccgc caacgttcac cacaacttct
      361 gtggtgtagc gatcctgacc agattgatcg gtccatttac gggtacgcag ctgaccttcg
      421 atataaacct gagaaccttt acgcagatat tcgctggcca cttctgccag tttgccgaac
      481 agcacaacgc ggtgccattc agtctgctct ttcatctcgc cggtcgcttt atcacgccag
      541 gattcggaag tagccagcgt aatgttggca actgcgccac catttggcat gtagcgtact
      601 tccgggtcct gacccagatt accaacgaga ataaccttgt ttacgcctct gctggccatg
      661 ttcgtgtctc ctgaaaaaaa atcgttctga ataagtgtaa acgcgcgatt gtaccattac
      721 caatagcgct tttactatgt tgtgacctcg gttccgggaa acaaacctgg ccagacattg
      781 ttacacaaca ctccgggtaa tgcattccaa tactgtatat tcattcaggt caatttgtgt
      841 cataattaac cgtttgtgat cgccggtagc accatgccac cgggcaaaaa agcgtttaat
      901 ccgggaaagg tgaatggata agatcgaagt tcggggcgcc cgcacccata atctcaaaaa
      961 catcaacctc gttatccccc gcgacaagct cattgtcgtg accgggcttt cgggttctgg
     1021 caaatcctcg ctcgctttcg acaccttata tgccgaaggg cagcgccgtt acgttgaatc
     1081 cctttccgcc tacgcgcggc agtttctgtc actgatggaa aagccggacg tcgatcatat
     1141 tgaggggctt tctcctgcca tctcaattga gcagaaatcg acgtctcata acccgcgttc
     1201 tacggtgggg acaatcaccg aaatccacga ctatttgcgt ttgttgttcg cccgcgtcgg
     1261 cgaaccgcgc tgtccggacc acgacgtccc gctggcggcg caaaccgtca gccagatggt
     1321 ggataacgtg ctgtcgcagc cggaaggcaa gcgtctgatg ctactcgcgc caatcattaa
     1381 agagcgcaaa ggcgaacaca ccaaaacgct ggagaacctg gcaagccagg gttacatccg
     1441 tgctcgtatt gatggcgaag tctgcgatct ttccgatccg ccgaaactgg aactgcaaaa
     1501 gaaacatacc attgaagtgg tggttgatcg cttcaaggtg cgtgacgatc ttacccaacg
     1561 tctggctgaa tcttttgaaa ccgcgctgga gctttccggt ggtaccgcgg tagtggcgga
     1621 tatggacgac ccgaaagcgg aagagctgct gttctccgcc aacttcgcct gcccaatttg
     1681 cggttacagt atgcgcgagc tggagccgcg actgttttcg tttaacaacc cggcgggggc
     1741 ctgcccgacc tgcgacggcc ttggcgtaca gcaatatttc gatcctgacc gcgtgatcca
     1801 gaatccggaa ctgtcgctgg caggtggtgc gatccgtggc tgggatcgcc gcaacttcta
     1861 ttatttccag atgctgaaat cactggcaga tcactataag ttcgacgtcg aagcgccgtg
     1921 gggcagcttg agcgcgaacg tgcataaagt ggtgttgtac ggttctggca aagaaaacat
     1981 tgaattcaaa tacatgaacg atcgtggcga tacctccatc cgtcgtcatc cgttcgaagg
     2041 cgtgctgcac aatatggagc gccgttataa agagacagaa tccagtgcgg tacgtgaaga
     2101 attagccaag tttatcagca atcgcccatg cgccagctgc gaaggaacgc gtctgcgtcg
     2161 ggaagcgcgc catgtgtatg tcgagaatac gccgctgccc gctatctccg acatgagcat
     2221 cggtcatgcg atggaattct tcaacaatct caaactcgca ggtcagcggg cgaagattgc
     2281 ggaaaaaatt cttaaagaga tcggcgatcg tttgaaattc ctcgttaacg tcggcctgaa
     2341 ttacctgacg ctttcccgct cggcagagac gctttctggc ggtgaagccc agcgtatccg
     2401 tctggcgagc cagattggtg cgggcctggt tggcgtgatg tatgtactgg acgagccgtc
     2461 tatcggcctg caccagcgcg ataacgagcg cctgttgggt acgcttatcc atctgcgcga
     2521 tctcggtaat accgtgattg tggtggagca cgacgaagac gcgattcgcg ccgctgacca
     2581 tgtgatcgac attggcccgg gcgcaggtgt acacggcggt gaagtggtcg cggaaggtcc
     2641 actggaagcg attatggcgg tgccggagtc gttaaccgga cagtacatga gcggtaaacg
     2701 caagattgaa gtgccgaaga aacgcgttcc ggcgaatccg gaaaaagtgc tgaagctgac
     2761 aggcgcacgc ggtaataacc tgaaagacgt gacgctgacg ctgccagtcg gtctgtttac
     2821 ctgcatcaca ggggtttcag gttccggtaa atcgacgctg attaacgaca cactgttccc
     2881 gattgcccaa cgccagttga atggggcaac catcgccgaa ccggcaccgt accgcgatat
     2941 tcagggactg gagcatttcg acaaagtaat cgatatcgac caaagcccaa ttggtcgtac
     3001 tccgcgttct aacccggcga cctataccgg cgtgtttacg cctgtgcgcg aactgtttgc
     3061 gggcgtaccg gaatcccgtg cgcgcggcta tacgccggga cgtttcagct ttaacgtccg
     3121 tggcggacgc tgcgaggcct gtcagggcga tggcgtgatc aaagtggaga tgcacttcct
     3181 gccggacatt tacgtaccgt gcgaccagtg caaaggtaaa cgctataacc gtgaaacgct
     3241 ggagattaag tacaaaggca aaaccatcca cgaagtgctg gatatgacca tcgaagaggc
     3301 gcgtgagttc tttgatgcgg tgccagctct ggcgcgtaag ctgcaaacgt tgatggacgt
     3361 tggcctgacg tacattcgcc tggggcagtc cgcaaccaca ctttctggtg gtgaagccca
     3421 gcgcgtgaag ctggcgcgtg agctgtcaaa acgcggcacc gggcagacgc tgtatattct
     3481 cgacgagccg accaccggtt tgcacttcgc cgatattcag caactgctcg acgtactgca
     3541 taaactgcgc gatcagggca acaccattgt ggtgattgag cacaatctcg acgtgatcaa
     3601 aaccgctgac tggattgtcg atctgggacc agaaggcggc agtggtggcg gcgagatcct
     3661 cgtctccggt acgccagaaa ccgtcgcgga gtgcgaagct tcacatacgg cacgcttcct
     3721 caagccgatg ctgtaatcgt taaggccgct ttccgagcgg ccttttcctt tcagagttgc
     3781 accagcaatt tacgtttttc ttccggcagt aaattcaccg cctgctgata agacgcatcc
     3841 accagataat agatttgcga atccggcagc gaaccgtcga gatacacggt gctccagtgc
     3901 gctttattca gatggcggct tgggcgcaca tcgctgtgct gctgacgtag cagctccgcc
     3961 agctccgggc tggttttcag cgaaacagct gggcgatttt ctacttcttt caccatcgca
     4021 aacagtacat cttccacttt gatctgcgtc gctttccagt cgttatgcac gctctgttct
     4081 gcgcctggtt ttgccatgca atattgtagc aactccgaaa tggtcatttt ttactcccct
     4141 tgtagtgtcg cgatgatgcg acgcgatccg ccgtggatgc gatgttcccc cagccaaatg
     4201 ccttgccagg tgccggtctg aatacgccct ttatgcaccg gcaatacaag cgatgttccc
     4261 agcattgagg atttgatatg agaaggcata tcgtctgctc cctcatagtc atgctcataa
     4321 tttccgttgt cgggaacggt gcggaggaaa aaacgctcca tgtcgtggcg tacggtggga
     4381 tcgcagttct cattaagtgt cagagaggcg gaggtatgtt gcagcaacag atgcagtaag
     4441 ccgatgttaa cgcgcggcat atcagccagc tgattcagaa tttcatccgt taccagatga
     4501 aacccacgag atttggcgct aagcgtgagc gtcttttgat accacatgtg ctgctccttg
     4561 ataaaactct cttaatcagt ttgcagcaag acagcgaaag gataaaggtg tgattaaaaa
     4621 aacagcattc gggagagtgt cacgctctcc cgctctgtca gtattctgaa ttgacgatca
     4681 cctcttcacc aaacgcaccc gcttgtggca agggtttgta ggtagagttg gaggcacgca
     4741 gaatgcggat accacgagcg ccgacatcgc gtgcggcggt aatatcgtta tcggaatcgc
     4801 cataaaaaat tcggatattt ttatcctgca gccattgcga ttttgtattt tgccctggtt
     4861 tatcgcccgc aaagatcacc ggattcatgc tggtggcagg aatatgaaaa ttatccgcca
     4921 gcgtttttga aaccgtttct gttttcgtcg ggctacgacc agtcacaaag aagatcgcgt
     4981 caccgcggcg tacatgcata tcaatcagct ggcgagcgac ctcttttgga atgctgaatt
     5041 catcccagcc attgttcatt ttttcccaga acacaggatt tttcagataa tcttcgcttt
     5101 ctggcgagaa ggtttttttg ccgcgccaga agcccggact ggaaaaaagt accgtgtcat
     5161 cgatatcaaa ccccaccgcc attggcggac gccctgcgag gctattttca atttgtgcga
     5221 ccgaaaccca atgaattggt gcctgttcag caagcctggc aacgttagta ccagggttaa
     5281 gcggtgaagg agatgaggcc agggcaacag cggaactgtt tagagcgaac aataagcaaa
     5341 cggcactgat tgcctgtgtg atcttgcgca tatttttccc taaatagtca gtatgttgaa
     5401 actttttatt gtgagatttg ttgcaaaaac tatctgacca taacgccagc tacaggggaa
     5461 aggaaggatt atctgcgttt tttgtgatga aaag
//
LOCUS       DAZUCE010000282         5470 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZUCE010000282 DAZUCE010000000
VERSION     DAZUCE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.283"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            156..491
                     /gene="eutS"
                     /locus_tag="ACHY03_004607"
     CDS             156..491
                     /gene="eutS"
                     /locus_tag="ACHY03_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFS3907759.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            504..983
                     /gene="eutP"
                     /locus_tag="ACHY03_004608"
     CDS             504..983
                     /gene="eutP"
                     /locus_tag="ACHY03_004608"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFS3907760.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            958..1659
                     /gene="eutQ"
                     /locus_tag="ACHY03_004609"
     CDS             958..1659
                     /gene="eutQ"
                     /locus_tag="ACHY03_004609"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFS3907761.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGHFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1656..2459
                     /gene="eutT"
                     /locus_tag="ACHY03_004610"
     CDS             1656..2459
                     /gene="eutT"
                     /locus_tag="ACHY03_004610"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFS3907762.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGD
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2456..3472
                     /gene="pta"
                     /locus_tag="ACHY03_004611"
     CDS             2456..3472
                     /gene="pta"
                     /locus_tag="ACHY03_004611"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFS3907763.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLVRAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRTITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEMNRESSLQ
                     TLVE"
     gene            3511..3804
                     /gene="eutM"
                     /locus_tag="ACHY03_004612"
     CDS             3511..3804
                     /gene="eutM"
                     /locus_tag="ACHY03_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFS3907764.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3911..4198
                     /gene="eutN"
                     /locus_tag="ACHY03_004613"
     CDS             3911..4198
                     /gene="eutN"
                     /locus_tag="ACHY03_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFS3907765.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4210..>5470
                     /locus_tag="ACHY03_004614"
     CDS             4210..>5470
                     /locus_tag="ACHY03_004614"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFS3907766.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNG"
BASE COUNT         1282 a         1423 c         1620 g         1145 t
ORIGIN      
        1 attcatgcgt tgcatatgaa agtttatgca ccacagcgaa tatctctcca ttcttagtga
       61 tctacctcac cttttaaacg cgcttgccga attttgttat ttactctgac gaaaaattgt
      121 cacgatacac gaaagttttt cacaggcggc gactcatgga taaagaacgc atcattcagg
      181 aatttgtgcc cggcaaacag gtcacgctgg cgcatctcat cgcgcatccc ggcgaggaac
      241 tggcgaagaa aatcggcgtt ccggatgcgg gcgcaattgg cattatgacg ctaactcctg
      301 gcgaaacggc gatgatcgcc ggtgatttag cattgaaagc cgctgatgtg catatcggtt
      361 ttctcgaccg cttcagtggt gcattggtga tttatggctc ggtgggcgca gtagaagaag
      421 cgttatcaca aaccgtcagt ggtttaggtc gcttattaaa ttatacgctt tgtgaaatga
      481 ccaaaagtta atacgaggca ttcatgaaac gaattgcgtt tgtgggtacc gtcggggcag
      541 gaaaaacaac gttatttaat gcgttgcagg ggaattatac cctcgccaga aaaacacagg
      601 ccgtggaatt taatgataag ggcgatattg atacgccggg tgaatatttt agccatcccc
      661 gctggtatca cgccttaatt accacgctac aggatgtgga catgctgatc tatgtccacg
      721 gcgcgaatga cccggaaagt cggttgcctg ccgggttgct ggatattggc gtcagcaaac
      781 ggcaaatcgc cgtcatcagt aaaacggaca tgccggatgc tgacgtcgcc gcaacgcgaa
      841 agttattgct tgagacgggc ttcgaagagc cgattttcga gctaaacagc cacgacccgc
      901 aaagcgtgca gcaactggtg gattatctgg catctctcac caaacaggag gaagcaggtg
      961 aaaaaactca tcacagcgaa tgatattcgt gaagcacacg cgcgcggcga acaggcaatg
     1021 tccgtggttc tgcgcgccag cattattacc ccggaagccc gtgaagtggc ggatttactg
     1081 ggttttacca tcaccgaatg tgatgaatcg atcccggtaa cggcgtccgt gcccgccagc
     1141 gtgccagccg ataaaaccga aagccagcgc atccgcgaaa ccatcattgc ccagctgccg
     1201 gaaggccagt ttaccgaaag cctggtcgcg cagctgatgg aaaaagtgat gaaggaaaaa
     1261 cagtcgctgg agcagggtgc aatgcagccg agcttcaaat cggtgaccgg caaaggcggc
     1321 atcaaagtga ttgatggcag cagcgtcaag tttggtcatt tcgacggcgc agagccgcat
     1381 tgcgtgggct taactgacct tgtgaccgga gacgacggca gcagcatggc cgctggtttt
     1441 atgcagtggg agaacgcatt cttcccgtgg accctgaact acgacgaaat cgatatggtg
     1501 ctggaagggg agctgcatgt tcgccacgaa ggagaaacca tgatcgccaa agcaggcgac
     1561 gtaatgttta tcccgaaagg ctccagcatc gaatttggta cgacatccag cgtgaaattc
     1621 ctgtatgtcg cctggccggc taactggcaa tccctatgaa agatttcatc accgaagcat
     1681 ggctaagagc gaaccatacg ctcagcgaag gcgcagagat ccatctccct gcggacagtc
     1741 gcctgacgcc ctctgcccgg gagttgctgg aaagccgcca tctgcgcatc aagtttattg
     1801 acgagcaggg ccgcctgttt gttgacgatg aacagcagca gccgcagccc gttcatgggc
     1861 tgaccagtag cgatgaacat ccgcaggcgt gctgcgaact gtgccgccag ccggtggcaa
     1921 aaaagccgga tacgctgact cacctgtcgg cggaaaaaat ggtcgccaaa agcgatccgc
     1981 gtctgggttt tcgcgcggtt ctcgacagca ccattgcgct ggcagtgtgg ctgcaaattg
     2041 aactggcgga accgtggcag ccgtggctgg cggatatccg ttcgcgtctt ggcaacatta
     2101 tgcgtgccga tgcgctgggg gaaccgctgg gcgatcaagc gattgtcggg ctttctgacg
     2161 aagatctgca tcggctttct catcagccgc tgcgctatct cgaccacgat catctggtgc
     2221 cggaagccag ccacgggcgt gatgcggcgt tgctcaatct gctacgcacc aaagtgcggg
     2281 aaaccgaaac cgtagcggcg caggtgttta tcacccgcag ctttgaagta ttacgcccgg
     2341 acatcttgca ggcgctgaac cgtctttcca gcacggtcta cgtgatgatg atcctgagcg
     2401 taacgaagca gccgctaacg gtgaagcaga tccaacaacg actgggggaa acgcaatgat
     2461 tattgaacgt tgtcgtgaac tggcgttgcg agcgcccgcc agagtggttt ttccggatgc
     2521 gttagatcaa cgtgtgctga aagctgcgca atatttacat caacaaggtc tggcaacgcc
     2581 cattctggtc gccaatccgt ttgaacttcg tcagtttgcg ctcagtcacg gcgtggcgat
     2641 ggacgggcta caggtgatag atccgcatgg caacctcgca atgcgggaag aatttgctca
     2701 tcgctggctg gtccgcgcgg gcgaaaaaac gccgccggat gcgctggaaa aacttaccga
     2761 tccgctgatg ttcgccgccg caatggtcag cgccggtaaa gcggatgtct gtatcgcggg
     2821 caacctctct tccacggcga atgtgctgcg tgccggatta cgcattatcg gcttgcagcc
     2881 aggctgtaaa acgctctctt ccattttcct gatgctgcca cagtacagcg gtccggcgtt
     2941 gggttttgcg gattgcagcg tggtgccaca gccgacggcg gcgcagctgg cggatatcgc
     3001 acttgccagc gctgaaacct ggcgcaccat caccggagaa gagccgcgcg tggcgatgtt
     3061 gtcgttttcc agcaacggta gcgcccgtca cccctgcgtt gccaatgtcc agcaggcgac
     3121 agaaatcgtc cgtgagcgcg caccaaagct ggtagtcgat ggcgagttgc agtttgacgc
     3181 cgccttcgtg ccggaagtgg cggcgcaaaa agcgcctgcc agcccgctac agggcaaggc
     3241 caatgtgatg gtttttccgt cgctggaagc cggaaatatt ggctacaaaa tcgcacaacg
     3301 actcggcgga tatcgtgccg tcgggccatt gatacaagga cttgccgcgc cgatgcacga
     3361 tctctctcgt ggttgtagcg tgcaggaaat tatcgagctg gcgctggtgg cagctgtgcc
     3421 gcgtcagaca gaaatgaacc gcgaaagcag tttacaaaca ctggttgaat gaacggtccc
     3481 gttctggacc ctgataaaga ggaaagcaca atggaagcat taggaatgat cgaaacccgg
     3541 ggcctggttg cactgattga ggcttctgac gcgatggtga aagctgcgcg cgtcaagctg
     3601 gttggtgtta agcagattgg cggcggcctg tgtactgcca tggttcgtgg tgatgttgca
     3661 gcctgtaaag cggcgaccga tgccggtgcc gcagcggcac agcgtatcgg cgagctggta
     3721 tctgtacacg ttatcccgcg tccgcacggc gacctggaag aagtgttccc gatcggcctg
     3781 aaaggcgata gcagcaacct gtaatcaccc tgtgtgacgt cgattcgggc gtcacgctct
     3841 ctcatttttc attttacctt ccgcggaggg tagggggagc ttttacctga aatacgccac
     3901 ggaggcgggt atgaaactgg cagtcgtcac tggacaaatt gtttgtaccg tacgccatca
     3961 cggactggcg catgacaaat tgctgatggt ggaaatgatt gatccacaag gtaatcctga
     4021 cgggcaatgt gccgtcgcca tcgacaatat tggcgcggga accggggagt gggtgttgct
     4081 ggtgagtggc agttccgccc gccaggcgca taaaagcgaa acgtcaccgg tcgatctgtg
     4141 cgtgattggc atcgtcgatg aggtggtgtc tggcggtcag gtaattttcc acaaataagg
     4201 cagaacatca tgaatcaaca ggatattgaa caggtggtga aagcggtact gctgaaaatg
     4261 caaagcagtg acacgccgcc cgccgccgtt catgagatgg gcgttttcgc gtccctggat
     4321 gacgctgttg cggcagccaa aattgcccag caagggttaa agagcgtggc aatgcgccag
     4381 ttagccattg ctgccattcg tgaagcaggc gaaaaacacg ccagagattt agcggaactt
     4441 gccgtcagtg aaaccggcat ggggcgcgtt gaagataaat ttgcaaaaaa cgtcgctcag
     4501 gcgcgcggca cgccaggcgt tgagtgtctc tctccgcaag tgctgacggg cgacaacggc
     4561 ctgactttaa ttgaaaacgc gccctggggc gtggtggctt cggtgacgcc gtccactaac
     4621 ccggcggcaa ccgtaattaa taacgccatc agcctgattg ccgcgggcaa cagcgtcatt
     4681 tttgccccgc atccggcggc gaaaaaagtc tcccagcggg cgatcacgct gctcaaccag
     4741 gcgattgttg ccgcaggcgg gccggaaaac ttactggtta ctgtggcaaa tccggatatc
     4801 gaaaccgcgc aacgcttgtt caagtttccg ggtatcggcc tgctggtggt gaccggcggc
     4861 gaagcggtag tagaagcggc gcgtaaacac accaataaac gtctgattgc cgcaggcgct
     4921 ggcaacccgc cggtagtggt ggatgaaacc gctgacctcg cccgtgctgc tcagtccatc
     4981 gtcaaaggcg cgtctttcga taacaacatc atttgtgccg acgaaaaggt actgattgtt
     5041 gttgatagcg tagccgatga actgatgcgt ctgatggaag gccagcacgc ggtgaaactg
     5101 accgcagaac aggcgcagca gctgcaaccg gtgttgctga aaaatatcga cgagcgcgga
     5161 aaaggcaccg tcagccgtga ctgggttggt cgcgatgcgg cgaaaatcgc ggcggcaatc
     5221 ggcctgaacg tgccgcaaga aacgcgtttg ctgtttgtgg aaaccactgc agaacatcca
     5281 tttgccgtga ccgaactgat gatgccggtg ctgcccgtcg tgcgcgtcgc caacgtggcg
     5341 gatgccattg cgctggcggt gaaactggaa ggcggttgcc accacacggc ggcaatgcac
     5401 tcgcgcaata ttgagaacat gaaccagatg gcgaatgcca ttgataccag cattttcgtt
     5461 aagaacggac
//
LOCUS       DAZUCE010000283         5463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZUCE010000283 DAZUCE010000000
VERSION     DAZUCE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.284"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..855
                     /locus_tag="ACHY03_004615"
     CDS             <1..855
                     /locus_tag="ACHY03_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFS3907767.1"
                     /translation="IEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGKSGAGKS
                     SLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDAEYEALY
                     RDILPELDLVLWLIKADDRAMSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEWDMA
                     GIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASP
                     LMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDTVVSVAR
                     AVWNRIFF"
     gene            1073..3457
                     /locus_tag="ACHY03_004616"
     CDS             1073..3457
                     /locus_tag="ACHY03_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFS3907768.1"
                     /translation="MHEKNITLLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRREALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVNRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            3454..4359
                     /locus_tag="ACHY03_004617"
     CDS             3454..4359
                     /locus_tag="ACHY03_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFS3907769.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGMTGEGAARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTAMLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            4356..5426
                     /locus_tag="ACHY03_004618"
     CDS             4356..5426
                     /locus_tag="ACHY03_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFS3907770.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGVQISMVSGASIG
                     ALNGAIIAASPDLSEAAARLEALWKHLGENQVLSVNRTVYFSLLKKLFQAMNLCQIPG
                     RAGVLLTTLLRHISTINGFENPMAQPLLSDGPLTGLMDHYLDTDALSDGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNSVFQHIQSLPRGQQKEALLASAALPLLFSPREVQG
                     KMYGDGGMGGWQNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWERRAFPDTTILEIRPR
                     KKLKQTGEEGKSGGLLSFTSAHTDTWRQQGYEDTMLTMEHIRKPLAARQALTRSEAVL
                     QKSLDITEGADSALRNAMARLK"
BASE COUNT         1374 a         1356 c         1559 g         1174 t
ORIGIN      
        1 attgaggcaa ttgaaaaacc gctctcctcc ctgccttact cgctttcccg tcacatcctg
       61 gaacatctgc gcaaactcac ccgtcacgaa cccgtgattg gcattatggg taaaagcggg
      121 gccggtaaat cctcactctg taatgcactg tttcaggggg aggttacccc ggtcagtgat
      181 gttcacgccg gcacccggga agtgcagcgc ttccgtctga gtggccatgg tcacagcatg
      241 gttatcactg acctgcccgg ggtgggcgag agccgggaca gggatgcaga gtatgaagcc
      301 ctgtaccgtg acattctgcc cgaattggac ctggtactgt ggctgattaa agccgatgac
      361 cgtgccatgt ctgtggatga gtatttctgg cgacacatcc tgcaccgggg acatcagcag
      421 gtgctgtttg tggtgacgca ggccgacaaa acggagccct gccatgaatg ggatatggcc
      481 ggcattcagc cttctcccgc acaggcacag aacattcggg agaaaacgga ggcggtattc
      541 cgtctgttcc ggcccgtaca tccggttgtt gccgtatcgg cccgcaccgg ctgggaactg
      601 gatacgctgg tcagtgcgct catgacagca cttcccgacc atgccgccag tcccctgatg
      661 acccgcctgc aggacgagct gcgcacggaa tctgtccgcg ctcaggcccg tgaacagttt
      721 accggtgcgg tggaccggat atttgacaca gcggagagcg tctgtgttgc ctctgttgtc
      781 cgtacggccc tgcgcgctgt tcgtgacacc gtggtctctg ttgcccgcgc ggtatggaac
      841 cggattttct tctgaacccg gtggtgatgc accagactct tgttacagat ttttaacccc
      901 atgaaaacac aatgcaaaca cagccatacc cgggttgtcc ccttgcgttg tgtttctcaa
      961 gttaatagaa tgcatcgatc attactatca tatcgatcgt tataaacgat catttgtggt
     1021 aaatgacctc ctccgtggtt gtcatgaaaa tgcgtctcag gaaggttaaa taatgcacga
     1081 aaagaacatc accctgcttt gtgatgaagc cgaccgactt ttgcaactga acattaatct
     1141 gctccggcaa atggttgatg aaccggatgt gttatctgac agtaagaacg aaaacagact
     1201 gctttttgat aaacagaaag cactgaaaag aattgaggag ctggagggcg aacaaatcaa
     1261 aaccgcccgc agggagatgg tgctggctgt tgtcggcacg atgaaagcag gtaaatcaac
     1321 taccatcaac gccattgtgg ggcaggaaat tctgcctaac cgtaaccgcc ccatgacctc
     1381 tgtaccgacg ctcatccgcc acgttcccgg aaaaactgag ccggtcctcc atctggaaca
     1441 tattcagccc gtccgcaatt tattaagcac actgcaacaa aaactcgaca ccccggcagg
     1501 acagcaagtc gcacagaccc tgcagcaaac cggggatacc tgcgaactgc tggatattct
     1561 ggcaaatgat ggctggctca aaaatgaata ccacggggag gaggaaatct ttaccggact
     1621 ggcatcgtta aacgatctgg ttcgccttgc tgcggcaatg gggagtgagt ttccttttga
     1681 tgaatacgca gaagtgcaga aactgccggt gatcgacgtg gaattcagcc atctggtggg
     1741 gatggatgca tgccagggaa cactcacact gctggatacc cccggcccta atgaggccgg
     1801 acaaccgcag atggaagtga tgatgcggga tcaactgcag aaagcctctg cggtgctggc
     1861 ggtgatggat tacacccaga tgaactcaaa agcggatgaa gacgtccgta aagagcttaa
     1921 tgccattgct gacgtatcag ccggccgcct gtttgtgctg gtcaataaat ttgatgagaa
     1981 agaccgcaat ggcgatgggg cagatgccgt acgccagaaa gttccggcaa tgctgaacag
     2041 cgatgtactg cccgcctccc gcgtttatcc aggctcatca cgtcaggcgt acctggctaa
     2101 ccgtgcgctt catgagttac ggaaaaacgg aacccttcct gttgatgaag cctgggtcga
     2161 tgattttgtc agggaagcct tcggtcgcat gaaaaaagaa tacgtctgta aagacagtga
     2221 aatggcaacc gaaggggcga cagacctgtg ggaaggctca cttatcgatc aactgataac
     2281 ggaagtcata cagagctccc attccagagc ggcggcactg gcggttgatt ctgccgcagc
     2341 aaaactgatg cagaatgcgg aaaatgtcag cgaatatctg tcattacgcc atcaggggct
     2401 acagcaaagc attcagtcac tgcagtcgca tatcaccagc ctgcttgcgg atatccggga
     2461 aatcgcggac tgtcaggagc aggtgaccgc tgatgtcaga atggccatgg aggagatcga
     2521 tgccagaacc cgggaattac tgacgggggt ctgcacctca ctggaagagg agttgaatga
     2581 ctatttcaga agcggtaaac gcaaagaaca gcaaatgctg gaggaagaaa attcagcgca
     2641 gccccgggag agaaacgcat tcgctttctt tcacgatatt ttcggaaccg gaaatcagca
     2701 tgacaggatg cgggactttg atccggacag ccccgaaata aaattcagtg atcgcaggga
     2761 agcccttgaa ctgatgacgc aaatcgaatc gaccgtgacc agcctgcacc gtgaggctga
     2821 agcacagttc cgtcctgaac tggaaaaaat cgtcaggggt attgaaacag gttttcgtgg
     2881 tacggccctg tacgccacag aaaatattgc cggacgcatc aatgcccgtc tggaggatga
     2941 aggtttcact gtaaaaatca gttttccggc agtcagccag ttacagaccc ggctcgcagt
     3001 aaaaacaaat ctgagcgcgc ttatggagga aagaacggag acagtaaccc gtcgctgtcg
     3061 acaggatggt gtctggggta cgctttgtcg atgggcaaac accagtgact ggggctggaa
     3121 agagtacagt gtggatgtga accgcagcgt gatcaatatc aacaaggtca gagaggaagt
     3181 gatgtcactg acccgggcat atttcgggga gctgcaggca tccattgagc aggatattaa
     3241 ccagcccgtc cgccaggaaa tcgatgcctt tttctgtaca ttcagggaga aagttgaaca
     3301 gctgcgtaac acgctgattc agagctctga agatcataaa cgcgatcagc aggcgcagga
     3361 acggcttacc gggcgacttc aggcattaaa tgaaagggtt cctgagctca ttactgacag
     3421 taaggcgtta agggaagagc tggagacaat gctgtgactt caccatttat tcaacaaatt
     3481 gctgataacc gggtatgtca ggtactcacc tgtcttcctg aaaaatttgt ggttgatttc
     3541 gcaaacggta tcgatgttgc acaggagcat atccgcacgg ccggagagcg cacgttcttc
     3601 cggcgtttaa aagaggggat gaccggcgaa ggcgctgcac ggcagaatgc catcaatgct
     3661 tcgcttgcac agggcgttga ggcatccctg cgctggctga cggagctgac aacgtcactt
     3721 gccaccacaa actacgcgat tacccgggta aacgacaggg tcagttcact ggtcagtgat
     3781 actgccatgc tggcgcatta ttccgcagat acgcgggagc agttactcac tctggctgat
     3841 caggttcacc agaaactgaa tcatcttgaa gaaaaactcc accgtgttga cctggttcag
     3901 cgggcacagt tacatcttga gcagatattc tcatggtgga gtgccgggcg atatgcgtcc
     3961 ttttcccctg ccggacgctg ctatgtggcg cttgaagagc ttcgctgggg cgcgtttggt
     4021 gatgtgatac gtcagggcga aacagggcag gttaaccagc tactggatat tctcagacat
     4081 aaagcattaa cgcagatggc acaggagagt ggcggtagtg caaccgtgcg tctgaacacg
     4141 ctggactggc ttggtggtca gggccgggag caggcagata acgagtggca tgacgcgatt
     4201 aactggctgg gggactggtg cagtgaagag cggcatccgg tgatctggtc aaccacgcag
     4261 gctgcagaac acttaccggt tcgtatgccc cgtctctgct ctgcagagcg cctctctgaa
     4321 agtatggttg atgaaatatt tcagaagggg gcggcatgag tacagaaatg aaaacggggc
     4381 tggtgctgtc cggtggcggg gcggtgggcg cttatcaggc aggtgtggtt aaggctctgg
     4441 cagagtgcgg cgtacagatc agtatggtct caggagccag tatcggggct ctcaatggtg
     4501 ccattatcgc ggcctctccc gatctgtcag aagccgccgc acgcctggag gcgctctgga
     4561 agcatctggg agagaatcag gttctgtcgg taaaccgaac ggtttacttt tcattgctga
     4621 aaaaattgtt ccaggcaatg aacctctgcc agatccccgg acgtgcagga gtactgctta
     4681 cgacgcttct tcgccatata tcgacaatca acgggttcga aaatccgatg gctcagccgt
     4741 tgttgtcaga tggacccctg acagggctga tggatcatta tcttgatact gatgctctgt
     4801 cagacgggct accgttgtat gtgtcgttgt accccacgga agggggaatg caggatatta
     4861 tcgactgcat tcgtgctgaa ctgggtgccg gaactacaaa aaactccgtt tttcagcata
     4921 tccagagcct gccccgcggg cagcagaaag aagccttact ggcgtcagca gcactgcccc
     4981 tgctgttcag tccccgtgag gtacagggaa aaatgtacgg tgatggtggt atgggcggat
     5041 ggcaaaatat gcaggggaat acccctgtga cgccactggt ggatgccgga tgcaatatgg
     5101 tgattgtatc gcatctgagt gacggttctt tatgggagcg ccgggctttt ccggacacca
     5161 caatccttga aatccgtccc cggaaaaagc tgaaacaaac aggggaagaa ggcaaaagtg
     5221 gcggtctgct cagttttaca tcggcacata ccgacacctg gcgtcagcag ggctatgaag
     5281 acacgatgct gacgatggag catatccgga aaccactggc ggcacgtcag gcactgaccc
     5341 ggtcagaggc ggtattgcag aaaagcctgg atataactga aggtgcagat tcggcactga
     5401 gaaacgcgat ggcccgactt aaataaagac gctccggaga aacgccaccg cctgtggtgg
     5461 tgt
//
LOCUS       DAZUCE010000284         5444 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZUCE010000284 DAZUCE010000000
VERSION     DAZUCE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.285"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..192
                     /locus_tag="ACHY03_004619"
     CDS             <1..192
                     /locus_tag="ACHY03_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907771.1"
                     /translation="DALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGKQLIVAV
                     REQDMKKMEAEFGRIIKIH"
     gene            472..621
                     /locus_tag="ACHY03_004620"
     CDS             472..621
                     /locus_tag="ACHY03_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3907772.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            905..1165
                     /locus_tag="ACHY03_004621"
     CDS             905..1165
                     /locus_tag="ACHY03_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFS3907773.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1390..1464
                     /gene="tap"
                     /locus_tag="ACHY03_004622"
     CDS             1390..1464
                     /gene="tap"
                     /locus_tag="ACHY03_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFS3907774.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1457..2314
                     /gene="repA"
                     /locus_tag="ACHY03_004623"
     CDS             1457..2314
                     /gene="repA"
                     /locus_tag="ACHY03_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFS3907775.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEDAVAAARRSRVVWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMK
                     RARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            3015..3208
                     /locus_tag="ACHY03_004624"
                     /pseudo
     CDS             3015..3208
                     /locus_tag="ACHY03_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3250..3579
                     /locus_tag="ACHY03_004625"
     CDS             3250..3579
                     /locus_tag="ACHY03_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3907776.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            3560..3841
                     /locus_tag="ACHY03_004626"
     CDS             3560..3841
                     /locus_tag="ACHY03_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3907777.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
     gene            4024..4308
                     /locus_tag="ACHY03_004627"
     CDS             4024..4308
                     /locus_tag="ACHY03_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFS3907778.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNPLPVPR"
     gene            4308..4583
                     /locus_tag="ACHY03_004628"
     CDS             4308..4583
                     /locus_tag="ACHY03_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3907779.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARMVQSLIQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(4849..5345)
                     /locus_tag="ACHY03_004629"
                     /pseudo
     CDS             complement(4849..5345)
                     /locus_tag="ACHY03_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1423 a         1385 c         1360 g         1276 t
ORIGIN      
        1 gatgcactga acgccagtga gcatattatt atttttaatt gtacaggatg gcctgatttg
       61 aataatgaat ctgccgtcat gcagtttgcc agagtggcac gaaaatcagg aaaacaactg
      121 atagtcgcag taagagaaca ggatatgaaa aaaatggaag cagagtttgg aaggatcata
      181 aaaatccact gacgccttcg cgttgctcag ttgtccaacc ccggggacgg gaaaaagcaa
      241 gttttccccg cccccggcgt ttctgtagtg aaaagcggga aatggaggga atggtcagta
      301 acagggaatg tggtattatc gcggcgggtg tctgagcctt tctggttcag gcaagacgca
      361 ggtaccagaa atgcgaagac cccacttgtt aatccattaa ctcgtgaggc ctgcatgaag
      421 tacttgaaca ctactgattg tagcctcttc cttgcagaga ggtcaaagtt tatgacgaaa
      481 tatgccctta tcgggttgct cgccgtgtgc gccacggtgt tgtgtttttc actgatattc
      541 agggaacggt tatgtgagct gaatattcac aggggaaata cagtggtgca ggtaactctg
      601 gcctacgaag cacggaagta agctgccggg cggggacgga agtccccgct ttccggaagt
      661 gtgaggtatt tcaggggcag acacccgaca tgccagaaac cgccggtccc gcccggggcc
      721 ggcacacagg ttaaggcatt tcctgttttt cagccatttc gcaacaaaaa tcacattaaa
      781 caacagtaat caccgttgat ttgtgcgcca acacagatct tcgtcacaat tctcaagtcg
      841 ctgatttcaa aaaactgtag tatcctctgc gaaacgatcc ctgtttgaga attgaggagg
      901 cgagatgtcg cagacagaaa atgcagtgac ttcctcattg agtcaaaagc ggtttgtgcg
      961 cagaggtaag cctatgactg actctgagaa acaaatggcc gctgttgcaa gaaaacgtct
     1021 tacacacaaa gagataaaag tttttgtcaa aaatcctctg aaagatctca tggttgagta
     1081 ctgcgagaga gaggggataa cacaggctca gttcgttgag aaaatcatca aagatgaact
     1141 gcagagactg gacatactaa agtaaagact ttactttgtg gcgtagcatg ctagattact
     1201 gatcgtttaa ggaattttat ggcgggccac gccgtaaggt ggcagggaac tggttctgat
     1261 gtggatgtac aggagccaga aaagcaaaaa ccccgataat cttctttaac tttggcgagt
     1321 cagaaagatt accggggcca tctaaaaacc gtatagccaa caattcagct atgcggggag
     1381 tatagttata tgcccggaaa agttcaagac ttctttctgt gctcgctcct tctgcgcatt
     1441 gtaagtgcag gatggtgtga ctgatcttca acaaacgtat taccgccagg taaagaaccc
     1501 gaatccggtg tttacacccc gtaaaggtgc cggaacgctg aagttctgcg aaaaactgat
     1561 ggaaaaggcg gtgggcttca cctcccgttt tgatttcgcc attcatgtgg cgcatgcccg
     1621 ttcgcgtggt ctgcgtcgac gcatgccacc ggtgctgcgt cgacgggcta ttgatgcgct
     1681 gctgcagggg ctgtgtttcc attatgaccc gctggccaac cgcgtccagt gttccattac
     1741 cacactggcc attgagtgcg gactggcgac agagtccggt gcaggaaaac tctccatcac
     1801 ccgggccacc cgggccctga cgttcctgtc agagctggga ctgattacct accagacgga
     1861 atatgacccg cttatcgggt gctacattcc gaccgatatc acgttcacat cggcgctgtt
     1921 tgccgccctt gatgtgtctg aggatgcagt ggcagctgcg cgccgcagcc gtgtggtatg
     1981 ggaaaacaga cagcgcaaaa agcaggggct ggataccctg ggtatggatg agctgatagc
     2041 gaaagcctgg cgttttgtcc gtgagcgttt ccgcagttac cagacagagc ttaagtccca
     2101 cggcatgaaa cgtgcccgtg cgcgccgcga tgcaggcaga acccgccagg acattgtcac
     2161 cctggtgaaa cggcagctga cgcgtgaaat cgcggaaggg cgtttccggg gaagcctgga
     2221 ggccgtgaag cgcgaaatcg accgtcgtgt gaaggaacgc atgataatgt cgcgtaacaa
     2281 caactacaca cggctggcca ctgcctcccc ctgagtttta cctcatctga ataatccggc
     2341 ctgcgccgga ggcatctgca cgcctgaagc ctgtcagcga acaaaaaaac agcaccacat
     2401 acaaaaaaca atctcatcat cccgcttctg gtgcatccgg cccccctgtt ttcgatacaa
     2461 aacatgcctc actgacgggg aattttgctt atccacattt aactgcaagg gacttcccca
     2521 taaagttaca accgttcatg tcataaagcg ccagccgcca gccttacagg gtgcaatgta
     2581 tcttttaaac acctgtttat atctccttta aaccacttaa ctacattcat ttaaaaagaa
     2641 aacctattca ctgcctgtcc tgtggacaga cagatatgca cctcccaccg caagcggcgg
     2701 gccccgaccg gagccacttt agttacaaca ctcagataca accaccagaa aaacccaagt
     2761 cccgcgcaga actgaaacca caaagcccct cctcataact gaaaagcggc cccgccccgg
     2821 cccaaagggc cggaacagag tcgcttttaa ttatgaatgc tgtaactaat catcttcatc
     2881 gctgtcagtc ttctggctgg aagtcctcag tacacgctcg taagcggccc tgacggcccg
     2941 ctaacgcgga gatacgcccc gacttcgggt aaaccctcgt cgggaccact ccgaccgcgc
     3001 acataagctc tatcatagct gacagcaggt atggctttgc agggtgggga tgggtaaggt
     3061 gaaatctatc aatcagtacc ggcttacgcc gggcttcggc ggttttactc cggtatcaca
     3121 tgtaaccaca gcgtgccgcc ttacatgcgc tgacgcgcat aaacaacaag ttgctacatt
     3181 agcttacagg ttaattcacc ttgcataaga ttagcatata ggttaatgtt tatcgaaatg
     3241 ggaggtcata tgtttagcgt tgtttatcac ccggaagcca gggaagaagc cacagcatta
     3301 cccataaaaa tcagggtgaa gtttgaccgt ctcatcagca aactggaata tgacgcccga
     3361 ttactgcggg aaccggatac aaagcccctg ggggatggac tttttgagat ccgcacgatg
     3421 gggacggaca tagccagagg tatctgggta taccacaaag gcaataccat catcatgctc
     3481 cgggttttca tcaagaagtc tcagaaaacg ccagcaaaag aaatcgatct cgccaaaaag
     3541 cggttagcgg aggtacttaa tgaaactggt aagccataaa gaactgcata acgagtggat
     3601 gcaggacgac gaatatcgcg ctgcatggca ggaagaagaa cgtaaggaga aactcaggga
     3661 gttgcttgca gaatggcgta agcatgacaa tttgacaaaa gcgcaggttg ctgaacgcat
     3721 gggggttact cctcctgtta tttcccgcct ggaaaacaac atcactaaag ccagcatcga
     3781 caccctgact cgctatgccc atgcgtgcgg tatcaaaaaa cctgttatta cgctgtactg
     3841 aactctgctc tatggctgcc agttctggag aaatcagtaa ctaaatttta attagctacg
     3901 ttatggtaaa tctgcctgat gtgtcgattt ctctgaaagt aattcggctt ctgtgggctg
     3961 cgtttgcctt gtgccacctg aatgtaatca ctatgattaa atatgattaa caactaatcg
     4021 gatatacaaa tgaaaaacaa taccgcacag gcaacaaaag taattaccgc gcatgtgcca
     4081 ttacctatgg ctgataaagt cgaccagatg gccgccagac tggaacgctc ccggggctgg
     4141 gttatcaaac aggcactttc tgcatggctt gcccaggagg aggagcgtaa tcgcctgacg
     4201 ctggaagccc tggacgatgt gatatccgga cagattatcg accatcaggc tgtacaggcc
     4261 tgggcagaca gcctcagtac tgacaatccg ttaccggttc cacgctgatg gaactgaagt
     4321 ggaccagtaa ggcgctttct gatttggcgc ggttatatga ttttctggtg ctggccagta
     4381 aacctgccgc cgccagaatg gtacagtccc tgatacaggc accggtcatt ctgttaactc
     4441 atccacgtat gggagaacag ttgtttcagt ttgaacccag ggaggtcaga cggatttttg
     4501 ctggcgagta cgaaatccgt tacgaactta atggccagac tatttatgta ttgcgcctgt
     4561 ggcacacacg agaaaacagg tagattgcgt gtggggacaa cttaacatag cctgcagata
     4621 ttaattcagg aacattatta tcaggccaga gattttgcat tccattaaat aatcttgacg
     4681 tataggaaat tgaaaaactg agcgataccc ctgtccgcgc cttatgccac gtgagctttt
     4741 ctgcttttcc caccactccg gtgttttctc tctatgtatg gtcggttttt ataccttaat
     4801 gggccgtttt ttatactttt cccggattgc aggcgcacct ctcctgcatc agccagggat
     4861 atacctcagc aggcttatat cccgcgcgtt tttcttcagc cctgatacat tcagccccgc
     4921 aatggcccgg cggtatacca tccggcagcc acacagcacg tattcgaacg ggtcacgact
     4981 caggaactgc gcttcaccat ttgtgcatag cacactttcg ccactgcttc cggtttatcc
     5041 atccccagag cacggtacac ctgcggcagc ttctctccac acacccggtt ggcaaggaac
     5101 ccgaagtacc tcaccatctt aaaaaacttc tccgggatgt gctgtttcag cctcgcgacc
     5161 agctcacgct gtgtcagcgt ttccgtcgcc gtttctcccg ttttgtggtc caggtaacgg
     5221 aagctcaggc tcgcccctcc gttgtaatgt gccagccggg aggctgctat cggcggcttc
     5281 ttcagataac gacccaggtg gcgcgccgta ttcctcccgt cggccgtttt cttcgacatg
     5341 tacatcaggc gcggaactca tcggggcgcg ggaatgaaat cccattcaga ctccggatag
     5401 attcaagcaa gcccacttat cgtcaaaatc ggtgttgcaa aaac
//
LOCUS       DAZUCE010000285         5373 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZUCE010000285 DAZUCE010000000
VERSION     DAZUCE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.286"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(45..890)
                     /locus_tag="ACHY03_004630"
     CDS             complement(45..890)
                     /locus_tag="ACHY03_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFS3907780.1"
                     /translation="MSDITVSRPEVVNGHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISKANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     MLPSQ"
     gene            complement(975..1172)
                     /locus_tag="ACHY03_004631"
     CDS             complement(975..1172)
                     /locus_tag="ACHY03_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFS3907781.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(1184..1672)
                     /locus_tag="ACHY03_004632"
     CDS             complement(1184..1672)
                     /locus_tag="ACHY03_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFS3907782.1"
                     /translation="MKLTLTPEADSVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            complement(1669..2046)
                     /locus_tag="ACHY03_004633"
     CDS             complement(1669..2046)
                     /locus_tag="ACHY03_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFS3907783.1"
                     /translation="MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            complement(2093..2470)
                     /gene="cbeA"
                     /locus_tag="ACHY03_004634"
     CDS             complement(2093..2470)
                     /gene="cbeA"
                     /locus_tag="ACHY03_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFS3907784.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPAITV"
     gene            complement(2546..2767)
                     /gene="yeeT"
                     /locus_tag="ACHY03_004635"
     CDS             complement(2546..2767)
                     /gene="yeeT"
                     /locus_tag="ACHY03_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFS3907785.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     NIPGVLAVFAERRKDSFGPYVRLMSITLN"
     gene            complement(2836..3312)
                     /gene="radC"
                     /locus_tag="ACHY03_004636"
     CDS             complement(2836..3312)
                     /gene="radC"
                     /locus_tag="ACHY03_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFS3907786.1"
                     /translation="MQQLSFLPGAMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(3327..3800)
                     /locus_tag="ACHY03_004637"
     CDS             complement(3327..3800)
                     /locus_tag="ACHY03_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFS3907787.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNVMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(3934..4134)
                     /locus_tag="ACHY03_004638"
     CDS             complement(3934..4134)
                     /locus_tag="ACHY03_004638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907788.1"
                     /translation="MKHIPEGVIQAPVSPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            complement(4134..4955)
                     /locus_tag="ACHY03_004639"
     CDS             complement(4134..4955)
                     /locus_tag="ACHY03_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFS3907789.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVQKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(5133..5264)
                     /locus_tag="ACHY03_004640"
     CDS             complement(5133..5264)
                     /locus_tag="ACHY03_004640"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFS3907790.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRR"
BASE COUNT         1208 a         1376 c         1482 g         1307 t
ORIGIN      
        1 aaggaatgaa gctaaaatac ataattgaat caattcttag tactctattg tgaaggtaac
       61 atttccggat agtgtctggc tataatatca tttagcctgt caaccagttc tggtttcctg
      121 aacgtgatgt gtactgagcc tttcttaaag tatttaatac tgaacatctc gtcttcatag
      181 tcctctttac cctgaacaga ctggatgtga tcatccagac gaagggtgat gttttcccgg
      241 ttgtcgggga tcggtttgcc gctgaacagg tgtagcattc tttccagatc ggcaagtcga
      301 tctgcctgtt gcccggtgat aaaatgaaac ccccaccggt cccacctcac taggttgttg
      361 acgataattt tactgccaaa tttacagggt gagttggttt tgtaattcca gctcagtccc
      421 ctgaagacgt tgatcacccc tcgctcaaac acctcatcct tattctggtg cagttgttca
      481 aatgtgctca gaatgttcgc tttactgatt tccggaaaat tatcgtattc cagtgaccgg
      541 taccatgtat cacgggcctg tgcatccatt aaggaaagca tccctgaacg ttgcatcagg
      601 tcacgccaga tttcgcgatc gagattgcgt gtgatggctt tcattgcggt ttcaggtttc
      661 tccatcagcc agcaaccgca gtgaaaattc tgtttcatgg cccagtccag tgcggtttta
      721 ccgccaatgc tttctgtcat tgctgagatt tcagccagtt gccggataag tgtgtcgatt
      781 tgcagcagtg tactctttcg tacagccaga atgtggagga tactggttga gcagataacg
      841 tccgtatgcc cgttgacaac ttccggacgg gagactgtga tatcagacat aatcgatatt
      901 cctttttgca gataaatgaa aacgccagcc ctgttcaggc tggcgtcagt ggaatgaagt
      961 ctggtgagtg agagtcaccg gttctggtgc agaagctgca actggcgcag tgtacggata
     1021 tcttccctgg cctgttcaat gcagggcagc agtgctgccg aatccgtttt atcctcatcg
     1081 ttgtcaaaga taattcccga ttcgcagtcg atattgtcct gcagccacgc aatcagaata
     1141 tccagcgctg tttccgtggt taatgatttc atgttgtttc tggttaccag tcgaaagtgg
     1201 gtaaacccgg caggcattca gcgctggcat ccagatgaat gagactgacg ccataacgtc
     1261 gggtaagtgt gacaaccaga cggcggaacg tttttgacag ccccaggcgt ttaagccgca
     1321 gaaccgggta tgaccacgca tcggtacgta acagatatcc gctacctgtg aaatgaatcc
     1381 attctgattc gccggtgtca ttcgtctgat gtgacagcga gtacagccag gcgttgtcct
     1441 ttgcagtgat atgcgcggta ctgcagcgta tgccggtcag ggcagcaaat ggtggcgtgc
     1501 agtctgttga gacccggtca gattcatcaa caacacgaag ggagtggcca ttgtcgcaga
     1561 ccacgttaat cagttcagcg agattaacac catcgacgtc aacgacaatt cgtcccatgt
     1621 tcagcgcctg cacgttaacg ctgtcagctt ccggcgtcag agtcagtttc attgtttcgc
     1681 ctccggatgc ttcccctggg taatgttatt taccgttctg taattgtcgc gggtcatcag
     1741 gccggtcgcc ctgcgagccc ggaggatatc gatgctgttt attaactgag aacgggtaca
     1801 ggcactgaat tccggctggt cggtacgcac cagtgcgtat ttttcaacga gaaagttcac
     1861 cgcgtcacac agtgaaatcc ctgcctcaat atgctgctca atcacacgtt catcagcgaa
     1921 cggtgtgtca ttcagcgtga gaccatagtg ctggtccagc agtcgggtga gcagtgtctg
     1981 ccagatggtg acgggagacg ggcagcccga tgcctcccgt acgtgagtgt cgggtaatgt
     2041 attcatctta agttctctgt aacaggtggt gataaagggg agatatccgg ggttatacgg
     2101 tgattgcggg cgctgccggt gtcggataaa cagccagata aacgtaacca caggagccta
     2161 gggtgtcggc ttcgcaggtc agccctttcg catacagtgt gacggtatgc tgatggcggg
     2221 gattcagttc accgccggtg agcatgagtt ccagttgttt catcagcagc ggaaaggcct
     2281 ggtccaggtg gtacgcatcc gcgtcgctga acaggcctct gataccggcg cggtcggcaa
     2341 ggtaatgcag gcggttaccc tcctgcacca gacgtgcccc gaaacagggc gtcacggtgc
     2401 agggcagccc ccaccagggg cggtcgtgat tgtcgtcggg atgcgttgtt ccggggagtg
     2461 tgtctgacac gataaagtcc tcacagaaaa tcagttaaga atggttcgat ggtggcgata
     2521 attctgctca tctgaatgcc ctgattcagt tcagggtgat gctcatcagc cggacatacg
     2581 ggccaaaact gtctttacgg cgttctgcaa atacagccag cacaccggga atattctgca
     2641 cttcacgacc ggtatacgct tcagtgctgc cgtgccagcg gtatttgccg gtacagaacg
     2701 gaaaaagacg ggatgccgga tgttgttggt gaatacgcat ggcttcacca cgggtgatga
     2761 ttttcatagt gtgatacctc taaagacaga agataaaagc gaaaacaggt atgatgtggt
     2821 tgtgacggtg acaggttaaa gcagaccgtg ttccgcaaag gagaaaacct ggctgccacc
     2881 gactatcaga tggtccggca cccggatgtc aaccagggcc agcgcctgta ccagacgttc
     2941 ggtgataagc cggtctgcct tactgggtgt gacttcaccg gacgggtgat tgtgtgccag
     3001 caccacggca gcggcattgt ggtacagggc gcgtttaatc acttcccggg gatggacttc
     3061 cgtgcggttg atggtgccgg taaagagggt ttcaccggca atcagctgat tctggttgtt
     3121 cagatacagc acccggaatt cttcacgctc cagtcccgcc atgttcagaa tcagccattc
     3181 ccgtgccgct cgggtggagg tgaaggccac gccgggttca tgaagatggc ggtccagggt
     3241 tttcagggcc cgcagaatga ggctgcgctc gccgggcgtc atcgcgccgg gcagaaagga
     3301 aagttgttgc attgtacttc tcctgctcag tcgatgatgc gcataatggc gctgcattcc
     3361 ggatgctgca gggcgtagtc ccgcagacgg taataatgaa ccgtcatggc ataacactcc
     3421 gtgcgacagg catgatgact gtacgtcatc agacaggcag caatgccggc ggcttccggg
     3481 ctcatttcag cgcggttacc gttcatgaca ttgaacagca cccatgtttc gtcatcatcg
     3541 tcatccggtt caggggccat aaatgccccg ccgttgttca gggtgtacag attccagatg
     3601 cccccgcagt agtcttcgca cagacggtcc atccagccga agacacgggg ctccagggtc
     3661 acccactgtg ggatgaggcc aaaatgctgc ggccagaagc tgatgcgctg ttcatcaggg
     3721 actatggtgg caaccagttg ttgcaggcca gtctccggtg cacaggctga agaggtggtg
     3781 ttctgagaca atgttttcat gtgtgaaatc cttgtaatgt gaataaatga gaggaaataa
     3841 aggccggggg ctgacaacat cacgttcatc actgaccgtg agacccggca atatcaggga
     3901 tgaatgatat tcggctgaaa ttccggacag ccgttaaccg ttccggtcac agacggatgg
     3961 ccagatgcca ttgcctgtac tcagtctgca cagactgagt tctccgggac ggtaaagcag
     4021 acgggtacac caggcgcaca ggtcatcatc agaaatggaa cgacatcccg gcagcgcctc
     4081 cggggcgtcg ggtgatacgg gtgcctgaat cacgccttca gggatatgtt tcatcagcgc
     4141 atgctctcca gcagcgtttc tgccatcacc cacaatgcgc ggttgagctt aatatcggtg
     4201 tcgatgctgt gaatggcacg ggtgtggata cgttttcctt ttgcactgcg accggaaatc
     4261 ccgcctttca gcatattctc ctggatggtc tgatatgcgc tccacaggtc tttaccgtaa
     4321 tcctcccgac gtcgtggcgt cagaatgtcg gcggtggtga cgggctgatg ttcgtcacca
     4381 taacggtaag tcagtgccgc ctgtgccagc gcctggcgtg ccggtggcgg caggaccagc
     4441 gactgcatgg catcacgttt ctcttcaatc cggtcaaaaa cgcccaccac ctcgtaagcc
     4501 ccttcaatga ctttctgcac cacatttccc cggtgtggaa cacgcacttc ccccagagac
     4561 tgaccacaga cgcacccgtt ctggcagacg aaccggaagt aacccggcag catctggtag
     4621 ctggaggtac cgtcatggga gttgagcaga ataatttcag ggacatgttc tccgtttatc
     4681 tctccggccc gccgcagacg cagcatgtgt tttgtgtatc cccggcggcc cgggtcgcgc
     4741 acacgggtct ggcaggcgaa aaagggctgg aagccttccc gctgcagact ttccagtacg
     4801 gtgatggtgg ggatgtacgt atagttctgg cttcgggatg tgtgtttctc ttccccgaaa
     4861 atccccggca catggtgcat cagctcttca tgtgtcagcg gacggtcacg gcgtatctgg
     4921 tttgcagcat aaccaaaacg actgactaat cgcatacttt gctccttatc ggtggttaag
     4981 atttactggt gcaataaatg aaaaagccgc gtctcccgga gaagacgcgg cctgatggac
     5041 gaaatcaatg acgtttattg tctgagaagc cctccactgg cgagccacgc attaagctgt
     5101 tttccggcat cagcttcaga gttccggcag cgtcagcgtc gctgaccttc cgcgttgcgg
     5161 ataacccgac ggaaatgcat tcccgggtca tcctggataa acacattgcg gtaagcggtt
     5221 atgacagcaa ccgcctgccc gtatgagaaa gatccttcag ccaggacata ctctgtgtgt
     5281 aacccggcat atctggtttc tcctgataaa tagcctctgc catacgttgt ggcagaggct
     5341 gaagcatgaa actgacttca gggatcagtt aac
//
LOCUS       DAZUCE010000286         5358 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZUCE010000286 DAZUCE010000000
VERSION     DAZUCE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.287"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            101..1444
                     /locus_tag="ACHY03_004641"
     CDS             101..1444
                     /locus_tag="ACHY03_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFS3907791.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            1669..3324
                     /gene="opgD"
                     /locus_tag="ACHY03_004642"
                     /gene_synonym="mdoD"
     CDS             1669..3324
                     /gene="opgD"
                     /locus_tag="ACHY03_004642"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFS3907792.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            3464..3688
                     /locus_tag="ACHY03_004643"
     CDS             3464..3688
                     /locus_tag="ACHY03_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFS3907793.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            3751..4287
                     /gene="rimL"
                     /locus_tag="ACHY03_004644"
     CDS             3751..4287
                     /gene="rimL"
                     /locus_tag="ACHY03_004644"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFS3907794.1"
                     /translation="MTETIKISELLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4282..5262)
                     /gene="ydcK"
                     /locus_tag="ACHY03_004645"
     CDS             complement(4282..5262)
                     /gene="ydcK"
                     /locus_tag="ACHY03_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFS3907795.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAMSDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARI
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
BASE COUNT         1362 a         1291 c         1327 g         1378 t
ORIGIN      
        1 attgatgttt tcgcggcgag atcacagttt gtaaattctt cccgccagag tgaatgcggt
       61 tacctacact ccagattact gaccactgga ggcagacact atggcgaaca gcatcacggc
      121 ggatgagatt cgggaacagt tttcgcaggc aatgtcagcc atgtaccagc aagaagttcc
      181 gcaatatggc acgctgctgg aactggtagc tgatgtgaat ctggctgtgc tggaaaacaa
      241 tcctcaactg cacgaaaaaa tggtaaatgc agacgagctg gcgcgactga atgttgaacg
      301 tcatggggcg attcgcgttg ggactgcaca agagcttgct actcttcggc ggatgtttgc
      361 cattatgggg atgtacccgg tgagctatta cgatctctcg caggcagggg tgccggtaca
      421 ttcgacagca tttcggccca ttgatgatgc ttctctggcg cgtaatccct tccgcgtttt
      481 tacctcctta ctccgccttg agcttatcga gaacgaaatt ttgcgccaga aagcggcgga
      541 gattctgcgt cagcgcgata tcttcacccc acgttgtcga caactgttag aggagtatga
      601 ccagcggggc ggttttaacg aaacacaggc acaggagttt gtgcaggagg ccctggaaac
      661 gttccgttgg caccagtcag caacggtaga tgaagaaacc tatcgcgcct tgcacaacga
      721 acatcggttg attgctgatg tggtctgttt tcctggatgc catatcaacc atctgacgcc
      781 acgtacgctg gatattgacc gggtgcagtc gatgatgcct gaatgcggaa ttgaaccaaa
      841 aattctgatc gaagggccgc cgcgccgcga ggtaccgatt ttactacgcc agaccagctt
      901 taaagcactg gaagagacgg tgttgtttgc ggggcagaaa cagggcacgc atactgcgcg
      961 ctttggtgaa attgagcagc gtggcgtggc attaacgccg aaagggcgac aactgtatga
     1021 tgatcttctg cgtaacgctg gaaccgggca ggataatctc actcaccaaa tgcatttaca
     1081 ggaaaccttc cgcacttttc ctgacagtga gtttttaatg cgtcagcaag ggttggcatg
     1141 gttccggtac cgtctgacgc cttcgggtga ggcgcatcgt caggcgattc atcccggaga
     1201 cgatccacag cccttaattg aacgtggttg ggtcgcggcg caacccatta cctatgaaga
     1261 tttcttgccc gttagcgcgg cggggatctt ccagtcaaat ctgggtaatg aaacgcaggc
     1321 acgcagccac ggtaatgcca gtcgcgaagc atttgagcag gcgttgggtt gtccggtttt
     1381 ggatgagttc cagctttatc aggaagcgga agaacgcagt aaacgtcgct gtggtttgct
     1441 ttaaattctg accatccgcc tttgcaaaaa tttgcctgat tttacaaacg aatcaggctc
     1501 atcccatcga cataaaaaaa atgccgattt atgcatattc tctcagttca acaattggat
     1561 tattaataaa tattgtctag agtgagcggt cataaataag cactttcttg ccgctgaaaa
     1621 cgaccagcgc gggaccattc acaacaccag aaggactcac tttcaggtat ggatcgtaga
     1681 cgatttatta aaggttcaat ggctatggcc gccgtgtgcg gtaccagcgg cattgcttct
     1741 cttttttctc aggcggcatt cgcggcagat tctgatattg ccgacggaca aacccagcgt
     1801 tttgacttct ccattctaca gtcaatggcg catgacttag cgcaaacagc gtggcgtggt
     1861 gcgcctcgtc cgttacctga cactctggcg acaatgacgc cgcaggctta taacagtatt
     1921 caatacgacg ccgaaaaatc gctctggcat aacgttgaga accgtcaact ggacgctcag
     1981 ttcttccata tgggaatggg attccgtcgc cgcgttcgta tgttttctgt agatccagca
     2041 acacatctgg cgcgtgaaat tcactttcgc ccggagttgt tcaaatacaa cgatgcaggt
     2101 gttgatacca aacaattaga agggcaaagc gatctcggct ttgccggttt tcgcgtgttt
     2161 aaagcccccg aactggcgcg ccgtgatgta gtatcatttc tcggcgcgag ttatttccgc
     2221 gccgttgatg atacatatca atacggtttg tcggcccgcg gcctggcgat tgacacttac
     2281 accgacagta aagaagagtt ccccgacttt accgccttct ggtttgatac ggtaaaaccg
     2341 ggggcaacta cctttaccgt ttatgcgttg ctcgatagcg ccagcattac tggtgcctat
     2401 aagttcacta tccattgtga gaaaagtcag gtgattatgg atgtggaaaa tcacctgtat
     2461 gcgcgcaaag acattaaaca gctgggcatt gcgccgatga ccagtatgtt cagctgcggt
     2521 actaatgaac gtcggatgtg cgacaccatt catccgcaaa ttcatgactc tgatcgtctg
     2581 tccatgtggc ggggcaacgg cgagtggatt tgccgtccgc tgaataatcc gcaaaaattg
     2641 cagttcaatg cttacaccga caacaacccg aaagggtttg gtttattgca actggatcgt
     2701 gatttctccc attatcagga cattatgggc tggtataaca aacgcccaag tctgtgggtg
     2761 gaaccgcgta acaagtgggg taagggcacc atcggcctga tggaaatccc aacaacgggc
     2821 gaaacgctgg ataacattgt ctgcttctgg cagccagaaa aagctgtaaa ggcgggtgat
     2881 gagtttgcat tccagtatcg tctgtactgg agtgcgcaac cgcctgttca ttgcccatta
     2941 gcgcgcgtta tggcgacgcg taccggcatg ggcggtttcc cggaaggttg ggcaccaggt
     3001 gaacactatc ccgaaaaatg ggcgcgtcgt tttgccgtcg atttcgttgg tggtgatctg
     3061 aaagctgccg cgccaaaagg cattgagccg gtgattacgc tttccagtgg ggaagcgaag
     3121 caaatcgaaa ttctctatat tgaacccatt gatggttatc gtattcagtt tgactggtat
     3181 ccgacgtcgg actccactga tccggtcgat atgcggatgt atctgcgttg tcagggcgac
     3241 gctatcagtg aaacgtggct gtatcagtat ttcccgccag cgcccgataa acgtcagtat
     3301 gttgacgacc gcgtgatgag ttaattgttt tttcttcggc accttcttcg ggaggtgccg
     3361 tctggttaaa cacgatcccg ctcgcatttt tccctaagtt aaatgagtaa tctgatggtg
     3421 tgtatttcag atacaccttg tcagccacta acagggagtg cgtatgtttc cagaatatcg
     3481 agatttaata tcccgtctga aaaacgaaaa tcctcgcttt atgtccttgt tcgataaaca
     3541 caataaactt gatcatgaaa ttgccagaaa ggaaggttcc gacggtcgag ggtacaatgc
     3601 ggaagtggtc cgcatgaaaa aacaaaagct acagttaaaa gatgagatgc tcaaaatcct
     3661 gcagcaggag agcgtcaaag aggtgtaaac tttcctaagc cgccagccag gcggcttttt
     3721 taacaactgc atggattgac tggagataag atgactgaaa cgataaaaat aagcgaatta
     3781 cttgaattac atgctgttgc agaaaatcac gtcactcctc tttatcagtt aatctgtaaa
     3841 aataaaacct ggttacagca gtcgctaaac tggccgcagt tcgttcagag tgaagaggac
     3901 acgcgaaaaa cggtgcaggg taatgtgatg ttgcatcaac gcggctatgc caaaatgttc
     3961 atgattttca aagaagatga acttatcggc gttatctcgt ttaatcgtat tgaaccactg
     4021 aataaaaccg ctgaaatagg ctactggctg gacgaatctc atcaggggca ggggatcatt
     4081 tctcaggcgc tgcaggcatt gattcatcat tacgcccagt ctggtgaact tagacgcttc
     4141 gtgatcaaat gtcgggtgga caatccgcaa agcaaccagg tcgctttgcg caatggtttt
     4201 atccttgaag gttgcctgaa acaggctgag ttcctgaatg atgcctatga tgatgtgaac
     4261 ttatacgcgc gtattatcga ttcataataa ccctgccagc ggcgttcgcg taatgcgctc
     4321 ctcgccgtta atgacttttg gaccacggac atgaaccgtg tcgccatcaa aggcttcaac
     4381 gacggcgtgg tcggttaact ccacatggtt ttcaattatc accgcacctg taatacggct
     4441 ctcaccttgt attacaacgt gctcgtcgag cagaatcggt ccaccacgta ccacggcatt
     4501 tccgccaatc aagacatgat gttttaacac acaattacct tccacaatgg catattccgc
     4561 cacctgcgaa ctgtaatgaa tcgttggaat ggcatcttct tctatgccag ctttcacctg
     4621 cgcgtgaccg tagactttag cgcaatcgca tagccagacg ttgttctctt cattaccttc
     4681 aatactggca aaatcaaaaa cttcggcgcg atgttcaata aaagcatacc ggacgacggc
     4741 atcgccataa atttgtgcct ggtggacaat acgcgaggcg ctaatccttg cgcgatcata
     4801 aatttgtagc aggagttgat ggtcgggcgt taagccttgt gctgcgacga tcatagaatg
     4861 ttgattaatt aaggcgtgac caaatattct acattgcccg caaaccagtg agtcatgaat
     4921 ggtgacactg tcacttatat atgcaccctg gctgatttca ctattatcga tccagacatt
     4981 atccgttgcg taaacctctc cccacagcac gctggtgccg gtgatacgcg tattaccgga
     5041 aatcaccgtt ccgccgaacg caatagcatt ctggtcgtaa atccagcaat ttccttcctg
     5101 tgctaacacc gtttcgcgat cgatccagcc gccagcggtt cctgctatca catcgttaaa
     5161 atcgctcatg gcgataatct gccgtaataa cacatttttt ttagtgccat cttcctgata
     5221 actaaaggct cgctgttctt cacttagacg atatttgcgc atagcgtttt cccacaggtg
     5281 acttactata accgtagcaa attctgcggc tctggctatg ctcgagaaaa ttccataaaa
     5341 tgcatttcaa atatactt
//
LOCUS       DAZUCE010000287         5332 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZUCE010000287 DAZUCE010000000
VERSION     DAZUCE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.288"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            1..217
                     /locus_tag="ACHY03_004646"
                     /pseudo
     CDS             1..217
                     /locus_tag="ACHY03_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(204..1706)
                     /locus_tag="ACHY03_004647"
     CDS             complement(204..1706)
                     /locus_tag="ACHY03_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFS3907796.1"
                     /translation="MNIRKLFCPGNTPRILLFLFFFVVSAITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYKQLNQHGADNWKILPGVPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMMGSHPNPCDRLHSWSNNYQERFPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRGK"
     gene            complement(1888..2628)
                     /locus_tag="ACHY03_004648"
                     /pseudo
     CDS             complement(1888..2628)
                     /locus_tag="ACHY03_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
     gene            3061..4887
                     /gene="atoS"
                     /locus_tag="ACHY03_004649"
     CDS             3061..4887
                     /gene="atoS"
                     /locus_tag="ACHY03_004649"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFS3907797.1"
                     /translation="MNYIKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAGKMNVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRQELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     LSFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     HHSQWQQVSLNALVEETLVLVQTAGVQARIDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            4884..>5332
                     /locus_tag="ACHY03_004650"
     CDS             4884..>5332
                     /locus_tag="ACHY03_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFS3907798.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSP"
BASE COUNT         1509 a         1319 c         1182 g         1322 t
ORIGIN      
        1 tggactcatt aacacccttt cataatgctt tattgtcaaa cacgttatgg ctgacatcaa
       61 aaaaaccgga tttcctctgc cagcgggtaa tcacctcccc ggtgttttcg gttggtctgg
      121 ttactccagt ctggtaatta gcaagataat cgatataaac agtggaaaac tcatcgtaca
      181 taatctggtg atgaacatta cgcttatttt cccctgaccg gaagaatcag aggctgcggt
      241 ttcagactgt ctgccggtac attcctctcc ccgttaaaaa ccataacggg ttcattatct
      301 tcgtctgtca gcggattgaa tggcggtata ttttcagtac gaatgccggt cagccactga
      361 aaaatacctg cgaaatgacg ggcactgatt tttctgctga cagactgatg agacgtgatg
      421 tcactggcgg taataatcag gggaacgctg tagcctccct gcacatgacc atcatgatga
      481 acaggattag cactgtcgct gaccgacaga ccatggtcag aaaagtaaag catggcaaaa
      541 tgacgggaat gccggcgaag gataccatca agctgcccga gaaagttatc cagtttactg
      601 atgctggcga ggtaacaggc aatcttccgg gggaaccgct cctggtaatt attcgaccag
      661 gagtgaagcc ggtcacacgg gttcggatga gaccccatca tgtgcaggaa tatcaccttc
      721 ggagaggatt tatccgccag tgcacgttct gtttcctgta acaacaacat gtcatccgtt
      781 ttacgggaag caaagctgcc tttcttgagg aaaacggtat gctccgcatc agaagcaata
      841 acagagatgc gtgtatcatg ctcccccagc tttccctgat tggatatcca ccatgtgctg
      901 tatcctgctt ttgctgccag cgccaccacg ttgttgccgg agtcagggtt ctgctcatag
      961 tcataaatca gtgtccggct cagggaaggt acggtactgg ctgctgccga tgtatagccg
     1021 tcaataaata aaccgggagc tgtattcagc cacggcgtgg ttggcacggg atagccatat
     1081 accgacatat aatccctgcg cacactctca ccagtgacga taacaatcgt gtcatacaac
     1141 ggtacacccg gcaggatttt ccagttgtca gccccgtgct gattcagttg tttataacgc
     1201 tgcatttcac gcaatgtgtc agttgtcccc acaacagttc ctttaaccat ccgcaacggc
     1261 cagctgttta cagagcataa tacgaacagc agcagtgcca gccagttacg gtgaccgcgg
     1321 tggtgtgtcc gccagaaaat caccatgaat accagaatcg cggcactgac cagaaaatga
     1381 taaacaggaa tcatcccggt aaactctgca gcctcatcag ccgtggtctg caacaacgcg
     1441 acaataaaac tgttgttgat tttaccgtac gtcatacccg caggcgcata cagtgcacaa
     1501 cagaacagaa ataacagagc tgtaatggat gtaagggtat ttctgtgtgc aaggagcaga
     1561 agaaggaaca gcagcaacac attcccggtg gcattcttct cagtgtatcc gcatgcaatt
     1621 gtggttattg cagaaacaac aaaaaagaat aaaaacaata aaatccgggg tgtgttgccc
     1681 ggacaaaaca atttcctgat attcatcgga gtatatcgac aacattatta tgaagagaac
     1741 aagataataa aaatcagaaa ttatctgtaa aacagataac agacaaccct gcagtaatag
     1801 actgcagggt atttctgttt aattgcagaa gatatgcaat tatcttaatt acagaatata
     1861 cgcaattatc gttcaggagc agtgtcgtca gaagttataa gtcataccaa gcatgatgtc
     1921 atgactttta acatcaactt ctgacttata tttatcgccc cactcgtcct tataactcac
     1981 actggcatca cctgcatcaa gatagcgata gctgaggtcc agcgcgatat ccggggtgac
     2041 gtcatagcgg acacctgcgc caaggctcca tgcaaagtta tcagcagagc ctgagcgtga
     2101 caacgattcg cgaccactgt ttccgtaccc ataatcccag atactgatac cggttgtttt
     2161 ctggtgaact ctggcgtagc caatccctgc agatatccat ggtgtgaatg cgctgtcatt
     2221 ccggaagtca tagtacgtat tcagcatcag tgtgttgact gacacttcat tcttcaggtc
     2281 atcacgccag taaccacctg accaactgtc tttatctacg ttatacttcg aatcagcttt
     2341 tccacgagcg taaaactcca gttccgtacg aaccggaata ctgaactgcg gataaaaatc
     2401 ataaccagcc gcgataccgc cactgaatac cgtatcatgg tcatcaccac ctttatattt
     2461 gtccgcctac tctccttcac catcgataag acgctggttg gaaagagata aaacggaagg
     2521 ccccgcctta ccggtcatat aaaaacctgt tttgctctca tcagccaagg cctgggctga
     2581 aaacataccc gccattgcaa atactgaaac cacaataacc tttttcattt caatcatctc
     2641 cgttggtttt accctacccg acaaagaggg gcacctattc taaaaccatg caaaagacag
     2701 ggtaaaaaga gaaatttcta tcgaatcgat cgacaaaatc aatcatacat gatgaagatt
     2761 tcttatcgaa tccataaaaa tagtgatagg taacgggtac tgcaggaaca atcagaaatg
     2821 gaagtttggg aaagtgccat agcactacgt cgttgctaac atagaggaac agtgctttgt
     2881 tgataagatc ctttatacga cgaatctatt ggatgaaaaa taaactacgt aatcacctga
     2941 ttctcactga tattgccaat tctggtagga ttgagtgatt cttcacttac aaaacgatgc
     3001 tcaggaatat gataattgtt atatatcgat gtatacagga caatattagt ggatctcagc
     3061 atgaattata taaagtggat ttacccacgc cgcttacgca atcaaatgat cctgatggca
     3121 atcctgatgg tcattgtccc aacgcttact attggttata tcgtagaaac ggaaggacgt
     3181 tcagcagtct tatctgaaaa agagaaaaaa ctttctgctg tggtcaacct gcttaatcag
     3241 gcactaggcg atcgctacga tctctacatc gacttaccac gtgaggagcg tatccgcgca
     3301 ttaaatgcag aacttgcccc cattaccgaa aatatcactc acgccttccc tggcatcggt
     3361 gctggttatt acaacaaaat gctggatgcg ataatcacct acgcgccttc agcgctatat
     3421 cagaataatg tcggcgttac cattgccgca gatcaccctg gtcgcgaagt catgcgtaca
     3481 aatacccctt tggtttattc aggcaggcag gtgcgcggcg atattttgaa ttcaatgctc
     3541 cccattgagc gtaatggtga aatcctcggc tatatctggg ccaatgaatt aaccgaagat
     3601 attcgccgcc aggccgggaa aatgaatgtg aggattatca ttgtgctcac cgccggtttg
     3661 ctgataagcc tgctgttgat tgtccttttc tcccgtcgcc tgagcgccaa tattgatatc
     3721 atcaccgatg gcctctcgac tctggcacaa aatattccca ctcgattacc acaattgccc
     3781 ggtgaaatgg ggcaaatcag tcagagtgtt aataacctcg cccaggcact gcgtgaaacg
     3841 cggacactta acgatctgat tattgaaaac gctgccgatg gcgtcattgc cattgaccgc
     3901 cagggtgatg taaccaccat gaacccggca gcagaagtca tcactggcta tcaacgtcag
     3961 gaactggtag ggcagcctta ctccatgttg ttcgacaata ctcagttcta cagtccagta
     4021 ctggatacgc tggaacatgg caccgaacat gtagcgctgg agctcagttt tccaggtcgt
     4081 gaccgcacca ttgaactcag tgtcactacc agtcgtattc ataacacgca tggtgaaatg
     4141 ataggtgctt tggtgatttt ctctgattta actgcccgca aagaaaccca gcgccgcatg
     4201 gcgcaagcag aacgcctcgc cacactgggt gagctgatgg ctggcgtcgc gcatgaagta
     4261 cgtaatccgc taacggctat tcgtggttat gtacagatct tgcgccaaca aaccagcgac
     4321 ctaatacatc aggaatatct gtccgtagta ctcaaagaaa tcgattcaat taacaaagtt
     4381 attcagcaat tgctcgaatt ttcacgtcca caccacagtc aatggcaaca agtcagcctc
     4441 aatgcattgg ttgaagaaac tctggtactg gtacaaaccg ccggcgtaca agcgcggatc
     4501 gacttcataa gcgaactgga taatgaatta agcccgatta acgccgatcg tgaactgctc
     4561 aaacaggtac tgctgaatat cctgatcaat gccgtccagg ctatcagtgc acgagggaaa
     4621 attcgcattc gaacctggca gtacagcgac tcacaacagg ccatttcgat agaggacaac
     4681 ggctgtggca ttgatctctc gctgcaaaaa aagatcttcg atcccttttt caccaccaaa
     4741 gcctcaggaa ccgggcttgg tctggcgtta agtcaacgca tcattaatgc ccatcagggt
     4801 gatattcgcg tcgccagttt gccgggctac ggcgcaacct tcactcttat tttaccgatc
     4861 aatccgcagg gaaatcagac tgtatgactg ctattaatcg catccttatt gtggatgatg
     4921 aagataatgt tcgccgtatg ctgagcaccg cttttgcact acaaggattc gaaacacatt
     4981 gtgcgaacaa cggacgcaca gcattacacc tgtttgccga tattcaccct gacgtggtgt
     5041 tgatggatat ccgcatgcca gagatggacg gcatcaaggc actcaaggag atgcgcagcc
     5101 atgagacacg gacccccgtt attctgatga cggcctatgc tgaagtggaa accgccgtcg
     5161 aagcgctacg ctgcggggcc ttcgactatg ttattaaacc gtttgatctc gatgagttga
     5221 atttaatcgt tcagcgcgct ttacaactcc agtcaatgaa aaaagagatc cgtcatctgc
     5281 accaggcact gagcaccagc tggcaatggg ggcacattct caccaacagc cc
//
LOCUS       DAZUCE010000288         5317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZUCE010000288 DAZUCE010000000
VERSION     DAZUCE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.289"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            87..1421
                     /gene="garP"
                     /locus_tag="ACHY03_004651"
     CDS             87..1421
                     /gene="garP"
                     /locus_tag="ACHY03_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFS3907799.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1437..2207
                     /gene="garL"
                     /locus_tag="ACHY03_004652"
     CDS             1437..2207
                     /gene="garL"
                     /locus_tag="ACHY03_004652"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFS3907800.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITTLVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2237..3127
                     /gene="garR"
                     /locus_tag="ACHY03_004653"
     CDS             2237..3127
                     /gene="garR"
                     /locus_tag="ACHY03_004653"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFS3907801.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3224..4369
                     /gene="garK"
                     /locus_tag="ACHY03_004654"
     CDS             3224..4369
                     /gene="garK"
                     /locus_tag="ACHY03_004654"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFS3907802.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGYNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4402..4778
                     /gene="rnpB"
                     /locus_tag="ACHY03_004655"
     ncRNA           4402..4778
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHY03_004655"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(5166..>5317)
                     /locus_tag="ACHY03_004656"
     CDS             complement(5166..>5317)
                     /locus_tag="ACHY03_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907803.1"
                     /translation="SDNTLEKNENSPDNGFEVGEYNTYEAYNSENQYLTREDYTYDYD
                     LLNAI"
BASE COUNT         1255 a         1261 c         1497 g         1304 t
ORIGIN      
        1 cattgtcgtt acggcataaa tgaaactcat aaataacacc ttccccataa taataaaaat
       61 cagcataagt acccgaggta aataaaatga ttctggacac cgttgacgaa aaaaagaaag
      121 gcgtgcatac ccgctattta atattactga ttatttttat tgttaccgcc gttaactacg
      181 ccgatcgtgc aacgctgtct attgctggta ccgaagtagc aaaagagttg cagttaagtg
      241 cggtttcgat gggttacatc ttttctgctt ttggctgggc ctacttgctg atgcaaatcc
      301 ccggcggctg gctgcttgat aagtttggct cgaaaaaagt ttacacctac agcctctttt
      361 tctggtcgct attcaccttc ctgcaaggct ttgttgatat gttcccgctg gcctgggcag
      421 ggatctccat gttctttatg cgctttatgc tcggcttctc ggaagcgcca tcattcccgg
      481 cgaacgcccg tattgtcgcc gcctggttcc cgacgaaaga acgtggtact gcctccgcca
      541 tctttaactc ggcacaatat ttctcgctgg cgctcttttc gcctctgctt ggctggctga
      601 ctttcgcctg gggctgggag cacgtcttta ccgttatggg ggtgattggt tttgtgctga
      661 cggcgctgtg gatcaaattg attcataacc cgacagatca cccacgtatg tctgcggaag
      721 agctgaagtt tatctctgaa aatggcgcgg tggtcgatat ggaccacaaa aagccgggca
      781 gtgcggcagc aagcggaccc aaactgcatt acatcaagca attgctctct aaccgcatga
      841 tgttgggcgt atttttcgga caatatttta tcaacaccat cacctggttc ttcctcacct
      901 ggttcccgat ttatctggtg caggaaaaag gcatgtcgat tctgaaagtg ggtctggtcg
      961 cctcgattcc agcactgtgt ggttttgcgg gcggcgtgct gggaggtgtc ttctcggatt
     1021 atctgatcaa acgcggttta tccctgaccc tggcacgtaa gctaccgatt gtgctgggaa
     1081 tgttgttggc ttccaccatc atcttatgta actacaccaa caacaccacg ctggtggtca
     1141 tgctgatggc gctggctttc tttggcaaag gatttggtgc gctgggctgg ccggtaattt
     1201 ctgacaccgc gccgaaagag attgttggcc tctgcggcgg cgtctttaac gtctttggca
     1261 atgttgcctc cattgtcact ccactggtga ttggctacct ggtaagtgaa ctgcactcct
     1321 tcaatgcagc actggttttc gtgggatgtt cagcgctgat ggcgatggtc tgctacctct
     1381 tcgtagttgg cgacattaaa cgtatggaat tgcagaaata agcaaaggta taagcgatga
     1441 ataacgatgt tttcccgaat aaattcaaag ccgcactggc tgcgaaacag gtacaaattg
     1501 gttgctggtc agcactctct aacccgatta gcactgaagt tcttggtttg gctgggtttg
     1561 actggctggt gctggatggc gaacatgcgc caaacgatat ctccacgttt attccgcagt
     1621 taatggccct gaaaggcagc gccagcgcgc cagtagtacg agtgccgacc aacgagccgg
     1681 taattattaa gcgtcttctg gatatcggtt tctataactt cctgattcct tttgtagaaa
     1741 caaaagagga agcagagcag gcggtggcat cgacgcgtta tccgccagaa ggcattcgcg
     1801 gcgtctccgt ttctcaccgc gccaatatgt ttggcaccgt ggcggattat ttcgctcagt
     1861 cgaacaagaa catcactact ctggtccaga tagaaagtca gcagggcgta gataacgtcg
     1921 atgctattgc cgctaccgaa ggcgtagacg gcatcttcgt cggccccagc gatctggctg
     1981 cggcattagg ccatctcggc aatgcctcgc acccggatgt acaaaaagca attcagcaca
     2041 tttttaaccg tgccagcgcg cacggcaaac ccagcggtat cctcgcgccg gtcgaagccg
     2101 atgcgcgtcg ttatctggaa tggggcgcga cgtttgtggc tgtcggcagc gatctcggcg
     2161 tcttccgctc tgccactcag aaactggctg atacctttaa aaaataacca ccaccgcaac
     2221 aagagagatg attgatatga ctatgaaagt tggttttatt ggcctgggga ttatgggtaa
     2281 accaatgagt aaaaaccttc tgaaagcagg ttactcgctg gtggttgctg accgtaaccc
     2341 agaagctatt gctgacgtga ttgctgcagg tgcagaaaca gcgtctacgg ctaaagcgat
     2401 cgctgaacag tgcgacgtca tcataaccat gctgccaaac tcccctcatg tgaaagaggt
     2461 ggcgctgggt gagaatggca ttattgaagg cgcgaagcca ggtacggtat tgatcgatat
     2521 gagttctatc gcaccgctgg caagccgtga aatcagcgaa gcgctgaaag cgaaaggcat
     2581 tgatatgctg gatgctccgg tgagcggcgg tgaaccgaaa gccatcgacg gtacgctgtc
     2641 agtgatggtg ggcggcgaca aggctatttt cgacaaatac tatgatttga tgaaagcgat
     2701 ggcgggttcc gtggtgcata ccggggaaat cggtgcaggt aacgtcacta aactggcaaa
     2761 tcaggtcatt gtggcgctga atattgccgc gatgtcagaa gcgttaacgc tggcaactaa
     2821 agcgggcgtt aacccggacc tggtttatca ggcaattcgt ggtgggctgg cgggcagtac
     2881 cgtgctggat gccaaagcgc cgatggtgat ggaccgcaac ttcaagccgg gcttccgtat
     2941 tgatctgcat attaaggatc tggcgaatgc gctggatact tctcacggcg tcggcgcaca
     3001 actgccgctc acagctgcgg ttatggagat gatgcaggca ctgcgtgcag atggtttagg
     3061 aacggcggat catagcgccc tggcgtgcta ctacgaaaaa ctggcgaaag tcgaagttac
     3121 tcgttaatga cgtcgtgccc ggatggcgta ttgcaatccg ggcctggaat ccaggccgaa
     3181 taagagaaac gccctccggc gtcatgtggt aacaggcata ggtatgaaaa tcgtaatcgc
     3241 cccagactct tataaagaaa gtttatctgc cagcgaggtt gcgcaggcga tagaaaaagg
     3301 atttcgggaa atttttcctg atgcacagta cgtttctgtt cctgttgccg acggtggcga
     3361 aggaacggtg gaagcgatga ttgcagccac ccagggttcc gaacgtcacg cctgggttac
     3421 agggccgctg ggcgagaaag tgaatgccag ttgggggatc tccggcgatg gcaaaaccgc
     3481 gtttattgaa atggcggcgg ccagtgggct ggagctggta cctgcggaaa aacgcgatcc
     3541 actcgtgacc acttcacgcg gcacaggcga gttaatcctg caggcgctgg agagcggcgc
     3601 gacaaacatt attatcggca ttggcggcag cgctacaaat gatggcggcg caggcatggt
     3661 acaggcgctg ggggcgaaat tatgcgacgc caacggcaat gaaattggtt ttggcggcgg
     3721 tagtcttaat actctgaatg atattgatat ttccggcctc gatccgcgct taaaagattg
     3781 cgtcattcgc gtcgcttgtg atgtcaccaa tccgctggtg ggctataacg gcgcatcgcg
     3841 catctttggc ccacaaaagg gagccagtga agcgatgatt gttgagctgg acaataacct
     3901 ctctcactat gccgaggtca ttaaaaaagc gctgcatgtt gatgtgaaag atgtccccgg
     3961 cgcaggagct gctggcggta tgggagcggc gctgatggcg tttcttggtg cggaactgaa
     4021 aagtggtatt gaaatcgtca ctacggcgct gaatctggag gaacatattc acgattgtac
     4081 gctggtgatt accggtgaag ggcgtattga cagccagagt attcacggca aggtaccgat
     4141 tggcgtcgca aacgtggcga agaagtacca taaaccggtg attggcattg cgggtagcct
     4201 gaccgatgat gttggcgttg tacatcagca tggtattgat gcggtcttca gcgtactgac
     4261 cagcattggt acgttggacg aagcattccg cggggcttat gacaatatct gccgtgcttc
     4321 acgtaatatc gccgcgacac tggcgattgg aatgcgcaac gcggggtgac aagggcgcgc
     4381 aaaccctcta tactgcgcgc cgaagctgac cagacagtcg ccgcttcgtc gtcgtcctct
     4441 tcgggggaga cgggcggagg ggaggaaagt ccgggctcca tagggcaggg tgccaggtaa
     4501 cgcctggggg ggaaacccac gaccagtgca acagagagca aaccgccgat ggcccgcgca
     4561 agcgggatca ggtaagggtg aaagggtgcg gtaagagcgc accgcgcggc tggtaacagt
     4621 ccgtggcacg gtaaactcca cccggagcaa ggccaaatag gggttcataa ggtacggccc
     4681 gtactgaacc cgggtaggct gcttgagcca gtgagcgatt gctggcctag atgaatgact
     4741 gtccacgaca gaacccggct tatcggtcag tttcacctga tttacgtaaa aacccgcttc
     4801 ggcgggtttt tgcttttgga ggggcagaaa gatgaatgac tgtccacgac gctataccca
     4861 aaataaagcg gcttatcggt cagtttcacc tgatgtacgt aataaaccgt tccggcgggt
     4921 ttcagattgt tgagtgcgct ttattcatgc cggatgcggc gtgaacgcct tagcctgcct
     4981 acaaaagcac gcaaattcaa tatattgcag agatggcgta ggcctgataa gcgtagcgca
     5041 tcaggcattt ttgtggttgc catcagtttc aaacccgcgt cgataagttt ttgcttttgg
     5101 tgcacagggt aagatgcatg aatatctacg gtattaataa tagactttgt aagaacttat
     5161 taagtctata ttgcgtttaa aaggtcgtaa tcatacgtat agtcctctct ggtaagatat
     5221 tgattctctg agttatatgc ttcatatgtg ttatattccc cgacctcaaa accattgtcc
     5281 ggagaatttt cgtttttctc gagggtgtta tctgaaa
//
LOCUS       DAZUCE010000289         5307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZUCE010000289 DAZUCE010000000
VERSION     DAZUCE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.290"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            99..1196
                     /locus_tag="ACHY03_004657"
     CDS             99..1196
                     /locus_tag="ACHY03_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFS3907804.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(1386..1769)
                     /gene="quuD"
                     /locus_tag="ACHY03_004658"
     CDS             complement(1386..1769)
                     /gene="quuD"
                     /locus_tag="ACHY03_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFS3907805.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1891..1995)
                     /locus_tag="ACHY03_004659"
     CDS             complement(1891..1995)
                     /locus_tag="ACHY03_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFS3907806.1"
                     /translation="MMFEFYMAELLRHRWGRLRLYRFPGSVLTDYRIL"
     gene            complement(1992..2354)
                     /gene="rusA"
                     /locus_tag="ACHY03_004660"
     CDS             complement(1992..2354)
                     /gene="rusA"
                     /locus_tag="ACHY03_004660"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFS3907807.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDTLTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2351..2641)
                     /locus_tag="ACHY03_004661"
     CDS             complement(2351..2641)
                     /locus_tag="ACHY03_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFS3907808.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLTGLCGTGTK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2634..2804)
                     /locus_tag="ACHY03_004662"
     CDS             complement(2634..2804)
                     /locus_tag="ACHY03_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFS3907809.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2804..3259)
                     /gene="ybcN"
                     /locus_tag="ACHY03_004663"
     CDS             complement(2804..3259)
                     /gene="ybcN"
                     /locus_tag="ACHY03_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFS3907810.1"
                     /translation="MNLLQDGIKLHRGNFTAIGQQIQPYLENGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATSAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3256..3357)
                     /locus_tag="ACHY03_004664"
     CDS             complement(3256..3357)
                     /locus_tag="ACHY03_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907811.1"
                     /translation="MRTYNPNSLLPSQMQKCTCDFLHPAFDLCGGES"
     gene            complement(3451..3996)
                     /locus_tag="ACHY03_004665"
     CDS             complement(3451..3996)
                     /locus_tag="ACHY03_004665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907812.1"
                     /translation="MDAGIASVVAAIIAAAAAGVGLVITKENKTSEFRQAWIDGLREE
                     LAELMENFLQLRTTPPEKLPEVAGKIYFLSAKVKLRLSSKNLTNEESQLLKIIEDYIL
                     KMDRSSNITDVVRQYFEYSSSVLKTEWERVKRGEKKYRVAITVSYSILVFLGLYFASR
                     FIPAISEKILEIIEFLNYSLF"
     gene            complement(4136..4462)
                     /locus_tag="ACHY03_004666"
     CDS             complement(4136..4462)
                     /locus_tag="ACHY03_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907813.1"
                     /translation="MKPKKLRAEQQYHLDLELVKKKPANRTEAKAHLAAQLRISKYKT
                     QASSKIRVGSFKGRKKVHFSQAEEDARKAMAKANAVRFSEGEVESVDTERISESNKRW
                     RGRTAD"
     gene            complement(4834..>5307)
                     /locus_tag="ACHY03_004667"
     CDS             complement(4834..>5307)
                     /locus_tag="ACHY03_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HFS3907814.1"
                     /translation="FVISASFTANAGVEKLGPWITKSEINKMTDQTDFVALNLSPDSY
                     NKAGTDRATSLVLRCSDNKTDAYLSFNDYMGSDNPRITVRLDGGKPVKSVWGGGEGGD
                     SAFAPQPIQFIKTLAKHKKAIFGFEPYGSTMQVVEFDLSEIDKVVEKISQSCNWK"
BASE COUNT         1447 a         1268 c         1065 g         1527 t
ORIGIN      
        1 actaaaactt acatcttgaa ataatcacat tgattagatg aatatttatc tcgcagtgac
       61 atcatttttt aataatagtt caaaaaaaag ggctcacgat gaaaaaatta acagtggcaa
      121 tttctgctgt agctgcatca gtactgatgg cgatgtctgc tcaggcagct gaaatttata
      181 ataaagacag taacaagctg gatctgtacg ggaaagttaa tgccaagcac tacttttcct
      241 ctaacgatgc agatgatggt gatactactt atgttcgtct gggcttcaaa ggcgaaaccc
      301 aaatcaacga tcagctgact ggtttcggtc agtgggaata tgaattcaaa ggcaaccgcg
      361 ctgaatctca aggttcctcc aaagacaaaa cccgtcttgc atttgcaggc ctgaaattcg
      421 gtgactacgg ctcaatcgat tacggccgta actacggtgt agcatacgac atcggtgcgt
      481 ggactgacgt tctgccagaa ttcggtggcg atacctggac ccaaacagat gtgttcatga
      541 ctggtcgtac cactggtgtt gcaacctatc gtaacaacga cttctttggt ctggttgatg
      601 gtctgaactt tgctgctcag taccaaggca aaaacgatcg tagcgatttc gataactaca
      661 ccgaaggtaa cggtgatggc ttcggtttct ctgctaccta tgaatacgaa ggattcggta
      721 tcggtgcaac ttatgcgaaa tctgatcgta ccgacactca agttaatgca gggaaagttc
      781 ttcctgaagt atttgcttcc ggtaaaaatg cagaagtttg ggccgcaggt ctgaaatatg
      841 acgctaacaa catttacctg gccactacct attctgaaac ccagaatatg actgtatttg
      901 ctgatcactt cgttgctaat aaagctcaaa acttcgaagc tgttgcacaa tatcagttcg
      961 atttcggtct gcgtccgtcc gttgcttacc tgcaatctaa aggtaaggat cttggagtat
     1021 ggggcgatca ggacttagtc aaatatgttg atgtaggtgc aacctattac ttcaacaaaa
     1081 atatgtctac tttcgttgat tacaaaatca acctgcttga caaaaatgac ttcactaaag
     1141 cactcggtgt aagcactgat gacatcgttg ctgtaggtct ggtttaccag ttctaatctg
     1201 attacgaaaa agatatgttg cgggaggctt tgcctcccca acatataagt ggctccctca
     1261 agccacttcc tttaggagca caaccttgct tctaactata taaaccttct gttatatatt
     1321 accctttatt tttgggggcg ttgcaacgcc ccatttttaa taacttttag taaacaactg
     1381 gcatattaat tagagttatt aacaacgata tccatctcta accggatatc taatgccatt
     1441 aacatccctt caattatacc ctcagccttc tgtaaccttt tcccgatata accatcagag
     1501 cagcaatgct tacctgccag tgacatgaat gtcataccga ctacataata atctactaat
     1561 aaatcgtgca aatcgctgtt gttctttttc agacgggcca tgcacccgca aatgatcatc
     1621 gcgtcatcgt cacaacattg cgggcgagat tttacttttg aaggaattaa tcccttaaaa
     1681 ccggcggcaa tggacgacca agtcacatct tcatgattat tagccgccca cgctccccaa
     1741 cgctcaagaa ccatctgaat atcacgcatc aacttactcc acaaaaatca gaccagaacg
     1801 ccaattacaa gcaaaaatca acaaaacagt attagttgat tgttatctct gacttcatac
     1861 tcctgctcct gtcagggttt tggcgtaact ctacagtatt cggtaatcgg tcaaaacaga
     1921 accggggaaa cgatataagc gcagacgccc ccagcggtgg cgaagaagtt ctgccatata
     1981 aaactcaaac atcattcatt ccccatttcg gtgatggtca gttccagcct cccacctttg
     2041 gtaacaggca tcttcacaac gcggtaatca acgacttgag catcatccag ccagaaacct
     2101 gctttagtga gtgtgtcaaa agcggctttt tgcagattat ccaggtcacg gcgacggcga
     2161 tccggcatgt ggcactcaat gcggattttc acaggcatag ccaggccgat atccagcatt
     2221 gcgtttttaa tgattcgggc gacgttatcg cggtatgcct gcccctctgc gctgacgtgc
     2281 gtgcgcccgc gattatggcg gtaatagcga ttattgctcg gaggccaggg taatgtgatg
     2341 ctgtaggtat tcacgcctta ataaccccct ctttcagcca gataacctgt gttctcgcca
     2401 taccttccag cgcgcattct tttgcatatg cagcatcgac aaaatgtgtg cggcggtcga
     2461 tttcgtcgtg gcaggcagaa catgcaatgg tggcaatcag gtctggcggt ttggtaccgg
     2521 tgccgcacaa tccagtcagc cggatatgtg ccagtacaga cgtttcaggg ttgccattac
     2581 atacgccagg gattcttacc tggcattccc gaccacgcgc tgcttttctc aaatcagcca
     2641 tgattcctcc ttgctgccag tcgcaaccat tttttatcaa ccaggctggc ggtatatccg
     2701 agcagtgttg gtatttcgga aggcttcagc tccggtttac gcttacgacg atttggtact
     2761 ctgtagatgt gtccgttcat gacacgaata agcggtgtag ccattacgcc tcctgcttgt
     2821 cgcgcagcag ctggaactcg cagctctgcg gaatagtcag gtggcagcca atattcaccg
     2881 cccaggcttc aaccttacac aggaagacat acatctctcc ggtatcaaga tcggaggtat
     2941 ggcgtaacga ctggatagta gtgatttcgc cggttacgac atcaaccagg tccttggttt
     3001 cataaccgag gtatgtgtgt ttgagagcat cttttaccca tgctgaagta gcgaacgatt
     3061 tccccctgct gatgaggtat tcactgattt cgctgtacca catgtggctg agtgcattct
     3121 gggaaagact gcgtttctca cgccacggtt taagcaccat gcgaaagcat tttccgttct
     3181 ccagataagg ctggatctgc tggccgatag cggtgaagtt accgcgatgc aatttgatgc
     3241 catcttgtag gaggttcacg attcacctcc gcagaggtca aacgctggat gcaaaaaatc
     3301 gcaggtacat ttctgcatct gtgaagggag aagagagttt ggattgtatg tgcgcataaa
     3361 cgtccccgtt tagcgcagaa gtcaccggag ttgttcaggt tccggtgact taattatgac
     3421 aagttgatta tggaaaatca aagcttgtca tcagaacaac gaataattta aaaactctat
     3481 aatttctaag attttttcac ttatcgctgg aatgaaacgt gaagcgaaat agagccccaa
     3541 aaaaaccaat attgagtacg aaaccgtgat agcgactctg tattttttct cgcctcgctt
     3601 cactctctcc cactcagttt taagaacact ggaactatat tcaaaatact gtctgaccac
     3661 atctgtaatg ttagatgagc gatccatttt taaaatgtaa tcctcaatga ttttcaaaag
     3721 ctgagactct tcattagtta agtttttact ggataaccta agcttcactt ttgcggaaag
     3781 aaaataaatt tttccagcca cctcagggag cttctcaggc ggagtcgtgc gtaattgtaa
     3841 aaagttctcc atcaattcgg ctaactcttc ccttaaacca tctatccacg cttgccgaaa
     3901 ctcagatgtt ttattctcct tagtgataac aagccccact ccagctgcag ctgcggcaat
     3961 aattgcggcg acaactgagg ctatccctgc atccataaat tacccctcaa aacaatatga
     4021 atatgaatat gattcgatgt cttcagcaat tgaaaactaa aactccctgt aatgtcacat
     4081 ctgcccatga atgtgttttc ttcatacagc tttgctgcaa tatctaaatc agacattagt
     4141 cagcggttct cccgcgccag cgtttgttac tttctgatat tctttctgta tcaacggatt
     4201 caacctcacc ttctgaaaac ctaactgcat tagctttagc cattgccttc ctagcatctt
     4261 cttccgcctg gctaaaatgc accttctttc ttcccttgaa actaccaacg cgaattttgg
     4321 aagaggcctg cgtcttgtac ttgctaatcc gtaattgtgc cgccaaatgg gcttttgcct
     4381 cggtgcggtt cgctggcttc ttctttacta attcaaggtc taaatggtac tgctgctcag
     4441 ctcttagttt cttcggtttc ataatatcac tctcaaacaa agtccattta tcatagaata
     4501 aaagccctct aagagctttg atttgtatct atgctaattc ccgtcaattt gttcttcatt
     4561 ttacctccag ctgttgtgct gctgcagtgg cattcaatag ttttccttct aagcatccta
     4621 caaaatcggt gacgagtcat gaccagttca cgtagtgcaa tgaaattact gcaatctatt
     4681 atgacgcaaa tgcgtcaata ctcgaatcaa ctgatcatcc tgccacgact gaaatctcaa
     4741 ataggccgtt tctcaagtaa gagattttga atgcattatt ggctactgaa ataaaaaacc
     4801 cagcgccaac tgggttcata tgaaattttt ttgtcatttc caattgcaag actgtgaaat
     4861 tttttccaca accttatcaa tctcagacaa gtcaaactca actacttgca tagttgatcc
     4921 ataaggttca aacccaaaaa tagctttttt atgcttagcc aaggtcttta taaattgtat
     4981 tggttgtgga gcaaatgcag aatcaccgcc ttccccacct ccccaaacac tcttgaccgg
     5041 ctttccgcca tctaaccgca ctgtaattct tgggttgtcc gaacccatat aatcattgaa
     5101 tgataaatag gcatctgttt tgttatcact acaacgcaac accagtgaag ttgcacgatc
     5161 agtacctgct ttgttatatg aatctggtga taaattaaga gccacaaagt cagtctggtc
     5221 agtcatttta tttatctcag actttgttat ccacggccct aacttctcta caccagcatt
     5281 tgctgtaaac gaggcactta tgacaaa
//
LOCUS       DAZUCE010000290         5269 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZUCE010000290 DAZUCE010000000
VERSION     DAZUCE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.291"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1205
                     /gene="intR"
                     /locus_tag="ACHY03_004668"
     CDS             <1..1205
                     /gene="intR"
                     /locus_tag="ACHY03_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3907815.1"
                     /translation="GRYIRIWFMFRGKRCRETLKGWEITNSNIKKAGNLRALIVHEIN
                     SGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNTMKKTKSQLKTL
                     RIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRTVDNYIALLCSL
                     LRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQSQNLWKFAVYSG
                     LRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQ
                     YKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVSSLGARWNAAVK
                     RAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQI
                     AMLNARLS"
     gene            1257..2192
                     /gene="ttcA"
                     /locus_tag="ACHY03_004669"
     CDS             1257..2192
                     /gene="ttcA"
                     /locus_tag="ACHY03_004669"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFS3907816.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2321..3694)
                     /gene="dbpA"
                     /locus_tag="ACHY03_004670"
     CDS             complement(2321..3694)
                     /gene="dbpA"
                     /locus_tag="ACHY03_004670"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFS3907817.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3724..3897)
                     /gene="ynaL"
                     /locus_tag="ACHY03_004671"
     CDS             complement(3724..3897)
                     /gene="ynaL"
                     /locus_tag="ACHY03_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFS3907818.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4172..5155)
                     /gene="zntB"
                     /locus_tag="ACHY03_004672"
     CDS             complement(4172..5155)
                     /gene="zntB"
                     /locus_tag="ACHY03_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFS3907819.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5169..5216)
                     /gene="ydaN"
                     /locus_tag="ACHY03_004673"
     CDS             complement(5169..5216)
                     /gene="ydaN"
                     /locus_tag="ACHY03_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFS3907820.1"
                     /translation="MLRNRARRLDYKIMR"
BASE COUNT         1475 a         1295 c         1266 g         1233 t
ORIGIN      
        1 gaggtagata cattcgcatc tggttcatgt ttcgaggaaa acgatgtcgg gaaacattaa
       61 aaggctggga gattacaaac agtaatatta aaaaggccgg aaatttaaga gcgctgatag
      121 ttcatgaaat aaactccggt gaatttgagt atttaagacg ttttccccag tccagcactg
      181 gggcaaaaat ggtgacaacg agagtcataa aaacgttcgg agagctttgt gatatctgga
      241 caaaaattaa agagacagag ttaacaacaa acacaatgaa gaaaacgaaa tcacaattaa
      301 aaacactcag aataataatt tgtgaaagta ccccgatatc acatattcgt tatagcgata
      361 tcttaaacta ccggaatgaa ctgctgcatg gagaaacgct ttacctggat aatccaagat
      421 ccaacaaaaa aggaagaacc gtgcgcacag ttgataacta tatcgccctg ctctgttcgc
      481 tgttgcgttt tgcgtatcag tcgggattta tatcaaccaa accatttgaa ggagtaaaaa
      541 aattacagcg aaacagaata aagcctgatc cgttatctaa aacagaattc aatgcattaa
      601 tggaaagtga aaaaggacag agccagaact tgtggaaatt tgccgtttac tcaggacttc
      661 gtcacgggga actggcagct ctggcgtggg aggatgtgga tctcgaaaag ggaatagtga
      721 atgtcagaag aaacctgacg atacttgata tgttcggtcc cccaaaaaca aatgccggga
      781 tccgaacagt aacactactg cagcctgctc ttgaagcact gaaggagcaa tacaaactga
      841 ccgggcatca tcgcaaaagc gaaatcacct tttatcatcg ggagtacggc agaaccgaaa
      901 agcaaaaact gcattttgtt ttcatgccca gggtgtgtaa cggaaaacaa aaaccttatt
      961 actcggtaag cagtttgggg gcaaggtgga atgcagcagt aaaacgtgct ggtattcgcc
     1021 gccgtaatcc gtaccatacg cggcatactt ttgcctgctg gctgttgacg gcaggagcga
     1081 acccggcatt tatagccagc cagatggggc atgaaactgc gcagatggtg tatgaaattt
     1141 acggtatgtg gattgatgac atgaacgacg aacagatagc catgttgaat gcgcggttat
     1201 cgtagttgca aagtttgccc ccaatttgcc ccatttagta ccagagaact gaaataatgc
     1261 aagaaaatca acaaattaca aagaaagaac aatacaatct gaacaaatta caaaaacgtc
     1321 tgcgtcgtaa cgtgggcgaa gccattgctg acttcaatat gattgaagaa ggcgatcgca
     1381 tcatggtttg cctctccggg ggtaaagaca gctataccat gctggagatt ctgcgcaatt
     1441 tgcagcaaag cgcgccaatc aatttttcgc tggtggctgt taacctcgat caaaagcaac
     1501 cgggcttccc ggaacacgtt ctgcccgagt atcttgaaaa gctgggcgtt gagtacaaga
     1561 ttgttgaaga gaatacttac ggtatcgtga aagagaagat tccagagggc aaaaccactt
     1621 gctcactgtg ttctcgcctt cgtcgcggta tcctttatcg taccgcaacg gaactggggg
     1681 cgacgaagat cgcgttgggt caccatcgtg acgatatcct gcaaacgttg ttcttaaata
     1741 tgttctacgg cggcaagatg aaaggtatgc ctccgaaact gatgagcgat gatggcaaac
     1801 atatcgttat tcgtccgctg gcctactgcc gcgagaaaga tattcagcga tttgccgatg
     1861 caaaagcgtt cccgattatt ccgtgcaacc tgtgcggttc acagcctaac ctgcaacgtc
     1921 aggtgattgc tgacatgttg cgtgactggg ataaacgtta tccagggcgt atcgagacga
     1981 tgttcagcgc gatgcagaat gtggtgccgt cgcatctgtg cgataccaac ctgttcgatt
     2041 ttaaaggcat tacccacggt tctgaagtgg ttaacggggg tgatctggcg tttgatcgcg
     2101 aagagatccc actacaaccg gcgggctggc agccagaaga agatgaaaat cagttggatg
     2161 agttacgact gaatgtggtt gaagtgaaat aaccaggata gcgcccgatg cgcaagcgta
     2221 tcgggctact cttatggagg ccggataaga cgcggccagc gtcgcatccg gcaatcccga
     2281 ataagatgtt tactcttgca cccggcaatt caacatttca ttattttaat aaccgcaccc
     2341 ggcacgtttt tcctttaatc ttcccgccct gtaactgttt ccatgcttta tgagcaacag
     2401 cctgacggac cgcgacatag acatgcgccg gatgcacggc gattttgcca atatctgcgc
     2461 catcaagccc gatatctcct gtcagtgcac ctaatacatc acccgggcgc attttggctt
     2521 ttttcccgcc atcgatacac aacgttgcca tttctgcttc cagcggcaca atggaactat
     2581 tagctggcgg cgtttgccag ttaagtttta tctgcaacat gtcagaaatg atattggccc
     2641 gctgtgcttc ttccggagca cagaaactga tcgccagacc gctatttcct gcacgagctg
     2701 tacgaccgat gcgatgtaca tgaacttcag ggtcccacgc cagctcaaag ttcaccacca
     2761 gctcaagcga tttaatatcc agaccacgcg cagcaacatc agtagcaacc agtacacggg
     2821 cgctaccgtt agcaaaacgt accagagtct gatcgcgatc gcgttgctcc agatcgccgt
     2881 gtaacgacaa tgcactttgc cctacttcat tcagcgcgtc gcagacagcc tggcaatctt
     2941 ttttggtatt gcaaaacacc acgcaagagg atggctgatg caagcttaat aaccgttgca
     3001 acagaggaat tttgcctttg ctggatgtct cataaaattg ttgttcaatg ggtggcaaag
     3061 catctgttga gtcaatttca atcgccaaag gatcgcgttg tactcgtccg ctgattgcgg
     3121 cgatggcttc cggccaggtt gccgaaaaca gaagcgtctg tcgagatgca ggcgcaaaac
     3181 ggatgacatc atcaatggca tcgctaaatc ccatatccag catgcggtcg gcctcatcca
     3241 tcaccaaagt attcagcgca tccagtgata ccgtgccttt ttgcaagtga tccagcaaac
     3301 gacccggcgt tgccacgata atatgcggcg catgttgcaa cgaatcacgc tgcataccga
     3361 acggttgacc accgcacaac gtcaaaattt tggtatttgg cagaaaacgc gccagccgac
     3421 gcaattcacc tgccacctga tccgccagtt cacgcgtagg acacagcact aaagcctggg
     3481 tttgaaatag cgacgcatca atttgctgta acaagccgag gccaaaagcc gccgttttgc
     3541 cgctgccggt tttcgcctgc acgcgaacat cttttccggc aaggatcgcc ggaagcgcgg
     3601 cggcctgcac cggcgtcatg gttaaataac ccaactcatt aaggttcgtg agttgggcgg
     3661 gaggcaaaac attcagggta gaaaaagcgg tcacaatcta ttctcgtggt catcgacgca
     3721 aagttagcag gcgcgtatcc tcgcagatct acgctcacga tgcgacaatt taatcggttc
     3781 ttcatcgggt ggtgggtcag gcatgggttg cgggcgaggg atcggatcgg gcactggaac
     3841 aggatcgcca ggaatcggtt cagggacagg aatttgcaaa taaataagtg tcgtcatatt
     3901 tccctccggt cattgggtgg actcttaaag ggtagacgct gataaataac aggcaaaaaa
     3961 aagccgactc atcaaagtcg gcgtcgtacg aatcaattgt gctatgcagt aattcaaaaa
     4021 aggaagtaag acaatatgga gcgcaacgcc catcgcttga cgttgcattc acctgcaatg
     4081 gagatattgc cctgaatggg tagagagttt attgacttcg ctcaaacttt gcggcgtttt
     4141 tgtatacaga cagccggaaa aattgctttt gttacaacca tttactacgg tgcaaccata
     4201 aagcaacacc accaataaga acaactaaca gaatacaaaa aattgaaaat ccgaattgcc
     4261 acccgccgcc agggatccca ccaaggttga cgccaaataa ccctgtcaga aaggtactgg
     4321 gtaaaaagac cattgccatc aatgacattg tataggtacg acgagctaaa ttttcctgca
     4381 tcacctgagc gatttcatcc gccatcacgc cagtccgtgc tatacaggcg tcgatttcgt
     4441 caaggccgcg cccaaggcga tcggcaatat cctgcatccg acggcgttgg tcatcgctca
     4501 tccacggcaa acgttcactg gcaagacgag catagacatc acgttgcggt gccatatagc
     4561 gacgcatcac aattaattgt ttgcgcagca gagccaggaa tccacgcggc ggaatttgct
     4621 gatcaaggag attatcttca aggtcgataa ttttatcgtg cagctgctcg ataaattcgc
     4681 tggaatgatc ggtcaacgca tcgcacacat ccaccagcca ccctccgcaa tcggtcggac
     4741 ccgtgccctc ttccagatcg ctcaccacat cgtccagcgc cagcactttg cgttgtcggg
     4801 tcgaaacaat taaccgcccg tccatatata cacgcatggc gaccagttga tcggggcgtt
     4861 catcggtgct gccgtttata cagcgcaatg taataagcgt gccttcaccg aggcggctga
     4921 ctcggggccg tgtgctctcg cccgccagcg catcacgtac gttattggga agcagcggtg
     4981 ttgtcgccag ccattgggcg ctgtcatggt gtacataatt aaggtggagc cagcagggat
     5041 gcgcttcatc aatcacatct gtattttcca gcggtttaac gccgcctcta ccatccagca
     5101 tccaggcaaa tactgcatcc gggacattaa cgtccgatcc cttaatcgct tccacagtgc
     5161 ctccatcatc aacgcattat tttgtagtct agccttctgg ccctgttacg caacatctca
     5221 tcaccccatt accctgaaat gattaataaa attctgtcta aattgaata
//
LOCUS       DAZUCE010000291         5160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZUCE010000291 DAZUCE010000000
VERSION     DAZUCE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.292"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1266
                     /locus_tag="ACHY03_004674"
     CDS             <1..1266
                     /locus_tag="ACHY03_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFS3907821.1"
                     /translation="MKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESATLPV
                     ITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLTLVL
                     PASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVLSLD
                     MAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAETWF
                     DRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQLYR
                     DYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSPILF
                     TENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTVYTG
                     GHDYAWWRGAIIDELANYNCRKVSNNNSV"
     gene            complement(1408..2532)
                     /locus_tag="ACHY03_004675"
     CDS             complement(1408..2532)
                     /locus_tag="ACHY03_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFS3907822.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKLL"
     gene            complement(3262..3459)
                     /locus_tag="ACHY03_004676"
     CDS             complement(3262..3459)
                     /locus_tag="ACHY03_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3907823.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(3525..3798)
                     /locus_tag="ACHY03_004677"
                     /pseudo
     CDS             complement(3525..3798)
                     /locus_tag="ACHY03_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3845..4411)
                     /locus_tag="ACHY03_004678"
     CDS             complement(3845..4411)
                     /locus_tag="ACHY03_004678"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS3907824.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLSEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            complement(4439..4681)
                     /locus_tag="ACHY03_004679"
     CDS             complement(4439..4681)
                     /locus_tag="ACHY03_004679"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS3907825.1"
                     /translation="MVSINHTRESSPYLSVPQDDFCIVNPGGCIVHFPANGRDIQDIN
                     APDFPEQYQRLLARLDYLTKPDAKASGQRNSGYSGF"
     gene            complement(4883..>5160)
                     /locus_tag="ACHY03_004680"
     CDS             complement(4883..>5160)
                     /locus_tag="ACHY03_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFS3907826.1"
                     /translation="EHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWH
                     CVMCERLFHGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
BASE COUNT         1519 a         1214 c         1057 g         1370 t
ORIGIN      
        1 atgaaaaact atcttttcca gacacccgaa gatatttgtg tacagttaaa aaaaatgaca
       61 catcctgtca caataagaac aacagatatt gctaatttct ggcactatct tgagtcagca
      121 acacttccag tgatcacaaa aagcaccact acagaaaatc gggaggttac atttctgtgg
      181 cgctcagaga aagcactgca aggcgtatat cttcgcctga atcgtgttac agataaaaaa
      241 gacgtcaaaa aaggactaat gactcatatc ccttcgacag atatctggat gctgacactg
      301 gtgttaccag cttcatatcg gggctcatac tcatttatag aaattcccac agatatgaca
      361 caaaaagaca tatttcaact aggaagtcgc ttctctccat tacccggtaa atctgatcca
      421 tttaacaaaa cagcagaaat aaatatacga ggattcggag aatcagtcct ttctcttgat
      481 atggctcctg aacaaaagga atgggatgat acttcccata aatgtacagg tattctttca
      541 acattacatt cctttgttgc aggatatcaa cgccggattc gtttatattt tcccctgaat
      601 ccaacatcag tacctcttgg attacttgtg ttacctgatg ctgaaacatg gtttgaccgg
      661 atagatatta cccgggcatt agatatggcc atcaccactg gtcgtatcac gccaatggca
      721 attatgggga tagacaatat taatgaatct gatcgtatga atatactggg aggcaataaa
      781 gaactcatct ttgatatagc ggaaaatctg ataccccagt tatacagaga ctatccggat
      841 atcgtatggg ctggtcgctc taatactgta ctggccgggc agagcctcgg tggagtgaca
      901 gcactgatgg cagctatata tgcgtcgaca acatttggta caatcattag tcactcacct
      961 tcaatgtggt ggaaccctga ccagggcagc ccgatcttgt ttactgagaa taatacctcc
     1021 tgggtaagtg agcagatact ttcagcgcct ccgaaagatg taaatattcg acttggagtc
     1081 ggttctttag aaggtacaac cgtctcacat gttcagcgat tgcatcattc gttaatcgca
     1141 gctggtttgg aaagtaacct cactgtctat accggtggtc acgattatgc ctggtggcgc
     1201 ggagcaatta ttgatgaatt agcaaattat aattgcagga aggtatcaaa taataactct
     1261 gtgtaaaata tcgttttatc gtccgataac tctttctgca atattattat gggagactat
     1321 ctcatttatt tagtgatgtc cttcatttat gtgatgaagg acattatatt actcataaca
     1381 tttctcgttg aaaatttcag caatacgcta caatagtttc ccgttgtttt ttaattttct
     1441 catcaaaacc tctgcaatct ctgcaggact gggttgttca gccatttcag cggctacctg
     1501 atctgaacag aagcgtagtg aatcatcata aagcaggcga tttaccaaat cactggtcag
     1561 tccatgcttg tccggaataa tcccacaccc acgcttcgca acaatttctg cattaacaga
     1621 gcgatctgca ccttcgccaa acacaatctg tggtattcca ctatacagcg ctgtcagggt
     1681 attacctgcg ccaccgtgat gaataaatcc atcagctcca ttaaggaata cacccatagg
     1741 tatccagtca accagacgta catttgatgg tagttttcgt aaaccagtac gagcatttat
     1801 tgcaagttgc aaaatgatat cagcatcaac ttcatttgca gaatccataa cccatgaaat
     1861 cagctccaga ccatcaacca ttggttttac agtgccaagg ctgataagta aacgtttccg
     1921 atcagaatcc ctgtcccacc atgtttcctt tacagcacct ccgttataag gaatatatct
     1981 cattgagata accggttctc cagcattttt aagaatgctc atacttgggg gagcaacatc
     2041 aatccatgct aaatcacata gtggaccgct gactccatgc cgcctgtaag cattgcttaa
     2101 agaacggttt aacatctgaa tatgggcaga tgtatgcgcg aatccaacag ccagcatcac
     2161 tgaaggaatt ctatatttag cagcaaccaa tgggcctgcc ggaccaagag ggggatagac
     2221 tattaaatct ggcctccatt ttcctgcaaa atcgatgagg ttatctgcca tttcatcgct
     2281 aaaaaaagag aaatgaccaa taatattact ttttttcctc aactcttcct gatggcgata
     2341 tccagcctct gaatctaaac ctggaactgc atcaaaaaca accagtccag cttctgctgc
     2401 tttattcgcg aatttgccag cgctactaat tactacatca tgtccaatta cacgaaaggc
     2461 ctgagccaga gaaatcaatg ggtataacaa accgtacagc ggtgggccaa taaaaagaat
     2521 acgttttcgc attaaaaaag tccgtcttct gcttacctgt ttatttttct atctctaaga
     2581 aaacataatt aatattattt ttatgtgagc ttatgcacaa aaataaccaa gtatattatc
     2641 tcctggtaga gatacaggat acagtttgta ggagctttct tttttgaaca atgatttgca
     2701 ggacagttat gctaaactag cggaatcagt atcacaacaa taaatgactg atcatacctg
     2761 aaatttgaat taacaggcat catgaatgtc aatagtctaa gcggtgacta cttatatcgc
     2821 ttgcataaat actaaacact gactagaaaa gtattggata accccgtcac taaaagtaac
     2881 gcttcggtta tttgctcgga atgtattaac gccaagattg ttagccgtta cgccttatat
     2941 cactggcgct gacaaatgtt tcttttctaa tttactgaac cggcgcagac acgcaggaaa
     3001 gcacgccggc accggtgtaa tgccagttct aatcaataag aatggaatga cgaatcagtc
     3061 aggaagggat aacaacccgc tgctgaacgg aaggtatcag caggaagatg aacgggaata
     3121 tcgccagcac caagggcagg gccgggttcc acaataccag cgctggcatt ttgcacattg
     3181 ccagcgtacc ggcattacaa cacgtttcat gctgctgccc cccagaattc ctcgattcgt
     3241 tgctacatcc actgctctac ttcacttcca ctgctccact tcacttttgt accagcggct
     3301 gctgcgcccc agtttgatgg gtttagggaa atggccttca ctgatcaact tgtaaaacca
     3361 tttgtcggtc ataccggtga aactggtgat atagttcatg tcgatcaggg aattatcggc
     3421 acgcagaaat acgctgtcgt ggcttgttgt agtcaacatg gtcagaatgt cctgtgagat
     3481 ttatgaagat tgtgcgaatg cggggaatct gctgagctgt gctttcagaa ctggcctgtt
     3541 acgggatacc agggattacc ggcggggtaa cgggcttccg gatcatacac acctcgatta
     3601 tcgcggacaa aatcactgaa cgcccatatc acctctttaa gtatgtcttc gcagtccggt
     3661 aaatgacgat ccagcgccac atcccgggtg gtacagcttt gatgcgcccg gtgacacaaa
     3721 gcccggattg ttccatacat cctgaatcaa acgccccaga ttaggggcgt cgaaatatgc
     3781 ctctctgacc attatattcc ggcgtatagg tagcaggtct gaagtgacaa tgcgtcacct
     3841 gacgttaaaa gtcactacac ccaagatgac gttcaacagc accatgcgat tcaatgtaag
     3901 cccgggctgt ctgttccagt acaccaggct cagcgttgta tgtgttagct gcatcaaata
     3961 ccaacgacaa cacttcagga tacacaacca gatgtgtaat ggagttatct tcactcaata
     4021 cttttcccca cgcctgctca atcagatttc ttagaaccac cacctcacga ctcttacacc
     4081 agacatcgtt attaagtagc agcaccataa gataaggagt ggtatcgtta gccacagctt
     4141 ccctactcca gagataatat aaaggggtgg gctcaacaga ttgatcttta cgtcgcttac
     4201 actgcaaata ttcagaaatg agtctatgca gttcaccagt aaaatcagcc atcagagagg
     4261 gaatggcctt attaataccc tgccctggta ttaatttaaa ttgtaataat ttaatttcag
     4321 gatgtgtggc tgcagcccga tacagagttg caaggacaca cttttgccag agggcgttac
     4381 aggaaagctt aacatttgat tctgtataca taataaatca ccttacagtt acaacaggtc
     4441 aaaaaccgct gtagccagag ttacgctggc ctgatgcttt agcatcgggc ttcgtcagat
     4501 aatccagacg tgccaataag cgctgatact gctcagggaa atcaggagca ttaatatcct
     4561 ggatgtcacg tccattagca gggaaatgaa caatgcagcc ccctggatta actatgcaga
     4621 aatcatcctg aggcactgat aaatacggag aggactctcg cgtatggttg attgacacca
     4681 cagtgcgaat tcggcggatg cgagatcacg gtacgattcc gcaccacact accaatcaat
     4741 accccgggtt ttattcaggt aagcaggact gcggatatcc ggtgtcgcgc ccttgtgtca
     4801 cgaacggggt gggtgcgaaa caccggataa aatacaggtt ggcaatacct ctgaacgccc
     4861 tgcgcagagc ggggatttcg gattaagtac tcgcacctcc gcagtcctca aacaagtctg
     4921 gctggtagct gtaaacagac ttcgtacata ccgctctgca ataaatcccc gtaccacagg
     4981 cctcacagaa cttcttccca tgaaaaagcc tctcacacat cacacaatgc cactccagca
     5041 cgaccggtaa tggcgataaa aacatcgcca tatcctcaat gtaagggtgg gacttttccg
     5101 gattcagcac cacacagtcc gccttctgtt gctcactcag ggcatgtaaa tcatgctcaa
//
LOCUS       DAZUCE010000292         5099 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZUCE010000292 DAZUCE010000000
VERSION     DAZUCE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.293"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(117..257)
                     /gene="yjjY"
                     /locus_tag="ACHY03_004681"
     CDS             complement(117..257)
                     /gene="yjjY"
                     /locus_tag="ACHY03_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFS3907827.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            353..1069
                     /gene="arcA"
                     /locus_tag="ACHY03_004682"
     CDS             353..1069
                     /gene="arcA"
                     /locus_tag="ACHY03_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFS3907828.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1129..2481)
                     /gene="creD"
                     /locus_tag="ACHY03_004683"
     CDS             complement(1129..2481)
                     /gene="creD"
                     /locus_tag="ACHY03_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFS3907829.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWSHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKDVTMDDQLRIWK
                     "
     gene            complement(2539..3963)
                     /gene="creC"
                     /locus_tag="ACHY03_004684"
     CDS             complement(2539..3963)
                     /gene="creC"
                     /locus_tag="ACHY03_004684"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFS3907830.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQYRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVIPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDHEHVTLKVLDTGSGIPYYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(3963..4652)
                     /gene="creB"
                     /locus_tag="ACHY03_004685"
     CDS             complement(3963..4652)
                     /gene="creB"
                     /locus_tag="ACHY03_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFS3907831.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4665..>5099)
                     /gene="creA"
                     /locus_tag="ACHY03_004686"
     CDS             complement(4665..>5099)
                     /gene="creA"
                     /locus_tag="ACHY03_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFS3907832.1"
                     /translation="MLGPLAHAEEIGSVDTVFKMIGPDHKIVVEAFDDPDVKNVTCYV
                     SRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGEVVFKKRTSLVFKS
                     LQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
BASE COUNT         1305 a         1412 c         1203 g         1179 t
ORIGIN      
        1 gacgaaagct agcatttaga tacgatgatt tcatcaaact gttaacgtgc tacaattgaa
       61 cttgatatat gtcaacgaag cgtagtttta ttgggtgtcc ggcccctctt agcctgttat
      121 gttgctgtta aaatggttag gatgacagcc gtttttgaca ctgtcgggtc ctgagggaaa
      181 gtacccacga ccaagctaat gatgttgttg acgttgatgg aaagtgcatc aagaacgcaa
      241 ttacgtactt tagtcatgtt acgccgatca tgttaatttg cagcatgcat caggcaggtc
      301 agggactttt gtacttcctg tttcgattta gttggcaatt taggtagcaa acatgcagac
      361 cccgcacatt cttatcgttg aagacgagtt ggtaacacgc aacacgttga aaagtatttt
      421 cgaagcggaa ggctatgatg ttttcgaagc gacagatggc gcggaaatgc atcagatcct
      481 ctctgaatat gacatcaacc tggtgatcat ggatatcaat ctgccgggta agaacggtct
      541 tctgttagcg cgtgaactgc gcgagcaggc gaatgttgcg ttgatgttcc tgactggccg
      601 tgacaacgaa gtcgataaaa ttctcggcct cgaaatcggt gcagatgact acatcaccaa
      661 accgttcaac ccgcgtgaac tgacgattcg tgcacgtaac ctgctgtccc gtaccatgaa
      721 tctgggtact gtcagcgaag aacgtcgtag cgttgaaagc tacaagttca atggttggga
      781 actggacatc aacagccgtt cgttgatcgg ccctgatggc gagcagtaca agctgccgcg
      841 cagcgagttc cgcgccatgc ttcacttctg tgaaaaccca ggcaaaattc agtcccgtgc
      901 tgaactgctg aagaaaatga ccggccgtga gctgaaacca cacgaccgta ctgtagacgt
      961 gacgatccgc cgtattcgta aacatttcga atctacgccg gatacgccgg aaatcatcgc
     1021 caccattcac ggtgaaggtt atcgcttctg cggtgatctg gaagattaat cggctttacc
     1081 accgtcaaaa aaaacggcgc tttttagcgc cgtttttatt tttcaacctt atttccagat
     1141 acgtaattga tcgtccattg taacgtcttt actggctttc attttcggca gtgaaaacgc
     1201 ataccagtcg atattacgag tcacaaacat catgccggcc agcgccacca ccagcacact
     1261 ggttcccaac aacagcgcgc tatcggcaga gttgagcagc ccccacatca caccatccag
     1321 caacaacagc gcgagggtaa acaacatgct gttgcgccag cctttcaata ccgcctgcaa
     1381 ataaataccg ttcattaacg ccccaatcag actggcgatt atccatgcca cggtaaaacc
     1441 ggtatgttca gaaagcgcca gcaagagcaa ataaaacatc accaatgaaa gccccaccag
     1501 caaatattgc attgggtgta aacgttgcgc ggtgagcgtt tcaaaaacaa agaacgccat
     1561 aaaagtcagc gcaatcagca gaatggcata cttagtcgcc cggtcagtta attggtattg
     1621 atcggctggc gtcgtcactg cgacgctaaa cgcggggaag ttttcccagc cggtatcatt
     1681 gcctgaagca aaacgctcac cgagattatt agcaaaccag ctgctttgcc attgcgcctg
     1741 aaaacctgac tcactaactt cccgtttggc tggtagaaaa tcacctaaaa aactgggatg
     1801 cgaccagttg ctggttaagg tcatttcgct attacgcccc gcaggcacca cagaaagatc
     1861 gccggtaccg cttaaattca gggccatatt cagcttcagg ttctgcttgc gccagtcccc
     1921 ttcgggtaaa gggatatgca cgccctgccc gccttgctct aacccggtgc cgggttcaat
     1981 ggtcagcgcc gttccgttaa cttcaggcgc tttcaccaca ccaataccac gcgcatcccc
     2041 gacgctaatc acaataaatg gcttgcctaa ggtgatattt ggcgcgtcga gttcgctaag
     2101 acgcgaaaca tcgaaatcgg cttttagcgt taaatcactg tgccagacct gaccggtata
     2161 aatccctatc ttgcgttctt ccacgttctg attgccatca accatcaatg actcaggtaa
     2221 ccaaaaatgg ataaaacttc gtttccgctc cacggtttta tcctcttcct gcaccgtata
     2281 aagctcggtc acaggaatag cgataagcgg cccaacgagt ttttgcggcc cgctggtact
     2341 ttggcgaatc gcatcttcca catcgctacg gtaatcggca cgttcgacaa tcacctgccg
     2401 aatcagcatt atcggaatca acaacagcaa tactgcacca aacagggtgg tcattttcca
     2461 gaacaggggg gatttcaaca tagtcttctc ctttgcaatg gcagcaggat acgaaggcta
     2521 tgtgggaaga atttgaagct atgtgaagtg acggtgaagt cgaagcgagg ccagcacgcc
     2581 accttcctgc acgttgcgca gcgtgacttc gccgttaaac aaacgggcga cctcactgac
     2641 aaacgccaac cccagaccgc tgcttttttg cccatttgca cgcggcaaag agtaaaagcg
     2701 ttcaaaaata cgtgacagcg cgtagtaagg aataccacta ccggtatcca gcaccttaag
     2761 cgtgacgtgt tcatgatcca cttcggcgct tagcgtgatg caaccgctct cgggagtaaa
     2821 atcgatggcg ttatccagta aattccccag cgcctgctcc agtaacgccg gttcagcagc
     2881 aacgttaacc tcagtaggaa taacatgcaa agtgatgttt ttttctgcca actgcacggt
     2941 gcgcgcttcg ctgacgcggc gaaataatgc cgccacatca acagcagtca gaacgacttc
     3001 ctgacgattc tccagtcttg cctggcgtag taacgtttcc accagcgcct gcattcgcgc
     3061 attttgcgtc agaatgttgt cagtaaaacg agctaccact tccggcggcg gaccttcgcg
     3121 taaaatttcc gccgcgccac gaatcgccgc cagtgggctt tttagctcat gggttaacgc
     3181 ataaacatac tgctcaatat agtttttccc ttccagcttc acgcgcatac tttccagcgc
     3241 ctgcgcaagc ttacgcaact cgctactgcc gagttcagga agaggaacgg gcttattgtc
     3301 agtgacggaa tcggcatagc gagtgagccg ggcaatggag cggttgatcc accaaaccat
     3361 gcctgcgcca atcaccagtg caatccccag caaaatggcg ctggcccata aaattcgccg
     3421 ctcgctgcgc ttaatgaccg gagccatcgc cgcgttcggt ttgcctacgc tcaaaacgcc
     3481 aataagccgc gagccgtcca taatcggcgc ggcaacatac atcaccgaac tttcgggatc
     3541 ggcaggattt tgcaacgtgc tgcgcgcacc atactgacca cgcaacgtta gccagacgtc
     3601 attccagcgc gaataatcct gtccaacggc tttatttgcc gaatcgaaca atactttgcc
     3661 ctgcgcatcg gtcatataga catgatattc attgcgcact ttgttaatgc caccgatatt
     3721 ggcgcgaaac gggcgatatt gtagctgatt aaacgcctgc gccagttgcc catgcgttgg
     3781 gtccccagag agcaaatcgg gacgcgccag ctccgccagc aacgttgcgg tatcgattaa
     3841 cgtcccctcc gttgctcttc gcacgcccgg tttaacttct ttgacaaaaa tagccagtac
     3901 gaaccaggcc gccaccgcca ccagtaaaaa atagcccagc agcaaccgca tgccgatacg
     3961 cattacaggc ccctcaggct atatcccatg ccgcgatgag tattaatcgg agaaagatcg
     4021 gggttgatgg cgcgcagctt ggcacgcaac gttttaatgt gggtatcgac ggtgcgatcg
     4081 taggtgtcct gcgcatcttc ccacacgcta tccattagtt gctggcggga ccagacgcgg
     4141 cctggcgact tgagtaacgt cttcagcaat aaaaactcat accgcgtcag cgttaatggc
     4201 gtgtcaaacc agctgatctg cgccgcgggt tcattcaatt caaaatgacc aatacggatg
     4261 acgggagacg gcgacgagaa cttcttcacc cgacgcagta aggtgcgcac cctggcgcac
     4321 acttcgcggg gtgaaaacgg tttagccaca tagtcgtcag cgccaatttc cagcccaagc
     4381 aggcgatcga cctcttcact tcgggccgtc aggaacagta caggtaacgc cggatggagc
     4441 gccagtaact ggcggcacaa ttcaaagccg ctgatatcag gcaaaccaac atcgagaatc
     4501 atgacgtcgg gaacctgctg gcgagcttta tccagcaccg gcaagcctcg ctcaaagacc
     4561 tcgacggcaa aaccttcctg ctgcaacatg tagaccagcg tgtcggctat cccttgctca
     4621 tcttccacta accagaccgt ttcccgttgc ataaatcgcc tctgttattg ccgccacggc
     4681 atgacaggaa ccgcgctaat cgcgtttttc ggcgaacctt ctacaacttt gtcggagtaa
     4741 gccagataag cgagcgcgtt gcgtttggcg tcataaaagc gcacgacctg taacgactta
     4801 aagaccaggg acgtgcgttt tttgaatact acctcgccct gagctttgcc gtttttaata
     4861 cgatccgaca gttcaatcgg cccgacttgc tgacaagaaa tagccgcatc ggaggtatct
     4921 tccgccagac ccaatccccc tttaatacca cctgttttcg cccggctcac ataacaggtg
     4981 acatttttca catcgggatc gtcaaaggct tccacaacaa ttttgtgatc cgggccgatc
     5041 attttaaata cggtgtcgac cgaaccaatc tcttctgcat gagccaatgg ccccagcat
//
LOCUS       DAZUCE010000293         5098 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZUCE010000293 DAZUCE010000000
VERSION     DAZUCE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.294"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..600
                     /gene="ykgH"
                     /locus_tag="ACHY03_004687"
     CDS             <1..600
                     /gene="ykgH"
                     /locus_tag="ACHY03_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFS3907833.1"
                     /translation="VILFIMAICMAMVLLFLYINKDNIKVSYSLKINQTTPGILVNCD
                     SNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQAS
                     IIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIK
                     HKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(843..1538)
                     /locus_tag="ACHY03_004688"
     CDS             complement(843..1538)
                     /locus_tag="ACHY03_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFS3907834.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTALNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAERLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1531..2958)
                     /locus_tag="ACHY03_004689"
     CDS             complement(1531..2958)
                     /locus_tag="ACHY03_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFS3907835.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2969..3688)
                     /locus_tag="ACHY03_004690"
     CDS             complement(2969..3688)
                     /locus_tag="ACHY03_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFS3907836.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4217..5071)
                     /gene="rclR"
                     /locus_tag="ACHY03_004691"
     CDS             complement(4217..5071)
                     /gene="rclR"
                     /locus_tag="ACHY03_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFS3907837.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRLAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQLAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         1350 a         1330 c         1091 g         1327 t
ORIGIN      
        1 gttatccttt ttattatggc tatctgtatg gctatggtgc tgttgtttct gtatatcaat
       61 aaagataata taaaagtgag ttacagccta aaaataaacc agacaacgcc aggcatactt
      121 gttaactgtg atagcaataa taactttgcc tgtcagacca caatgactga agatgttatt
      181 cagcgaatta ctacattttt tcacaccagc ccagatgtca agaacagaga aataaagctg
      241 gaatggtcag gagataagag agatttacca actgctgaag cggaaatatc tcgcgtgcag
      301 gcgtctatta tcaaatggta tgcgtcagaa taccataatg gcaggcaagt tctcgatgag
      361 atacaaacgc cttcagcaat taacagtgag ctttatacaa aaatgatata cctgaccagg
      421 aactggtcat tgtatccgaa cggtgatggc tgtgtaacta taagctcacc agaaataaaa
      481 cataaatacc ctgctgccat ttgcctggct ctgggatttt ttctaagcat tgtgatttct
      541 gtaatgtttt gccttgtcaa aaaaatggta gatgaatacc aacaaaactc tgggcagtaa
      601 taaacagcat tctgatagag agtgcctgta taaagctgaa gtgacaatga ccgggaaggg
      661 aacccgtttt caggaatgga tgcctgtctt tttcactgta aatatcctgg cattagtaat
      721 gccaggatat ttacagaaga ggagcataac catgacggaa cggggttaag tcaggttgtc
      781 cttaacgcta ttttcaacct cgtatgcctt cttcaggttt atgtccagac ttcatacctc
      841 tctcagcaat cctcaataat cagatacacc gctttcaccg gaccatgaac cccgacgact
      901 ttgataagct caatgtccgc cgttgagctg ggaccactga tgatgttaat acatgacggc
      961 atgcgttcac cggcctgcgc tttctgatgc aatctttctg cgagttgcgc tacacgcggc
     1021 agaatagtgc ttttacgcag gataaaaaga gaagattccg ggagcaggct caatgaacgc
     1081 ccgcgctcgg cggcggaaaa aagaaccacg cctcccgatt cggttaaacc atattcagca
     1141 tacacgacac ccactttagc ctgctctgcc tgcgagatat tctcggcacc tttcgccgga
     1201 tcccaaacaa cggcattgca ttcctgctgc aaacgttcgc taatccccaa ttcctccagc
     1261 ctcgtgtcac cgctaatcac gaccgactga tctcccagct ctttacacag acgtattgca
     1321 gcttctgccg ccttcgcctc gctggtcagc tcacagcgcg tcaacataac atcgctggca
     1381 aactgaataa acgcgtcaca gcgctgctgt tggttaagtg cggtaagcct ctcgttagca
     1441 tagttgttaa gcggcgcatc ttctgcttgc ggttcaagtc gcaacgggcg acccagtgcc
     1501 tgagcaacgt tattcaaaaa ttcgctccga ttatccattc tttttctcct gcgcctgatg
     1561 tttcttaaac caactacgga aactctctcc gtcagcttca ggaagatcgc gtgcttccat
     1621 ccagtcgcta atcgcgccaa atttgagtgg tgttttgccg ccattgataa accagcttgc
     1681 cgcatgagca ccggccatca tcccgacttt ccacaatcct ggatgactat tggcataagc
     1741 gaacattttt atcgcccgtt gctctgcttt tgcggtgatc cctttttcag ccatcacccg
     1801 acgatgacgc aaaatcagtt ttgacagcgg aatacgcacc ggacacacgc tgtcacaagc
     1861 agtgcataaa gagcaggcgt agggtaaatc tttaaaatct ttatagccgc caagtagcgg
     1921 agaaatcacc gcaccaattg gccctggata aatagagcca tatccatgac cgccaatatg
     1981 gcgatatgcc ggacaagtat tcatacaagc cccgcagcga atacagcgca gcacatcccg
     2041 aaattcagag gccagcacct cagaacgccc gttatcgaca ataaccagat gaaactcttc
     2101 aggaccatca acgtgccccg cttcgcgcgg tcctgtcagc caggtgttgt atcccgtcaa
     2161 acgtgcacca acggcactgc gcgccagcat ggtgatcaat acatctacct cggcaaacgt
     2221 gggggcaata cgctccattc ccatcactgc aatatgcgtt ttaggcagcg tggtacacat
     2281 tcgcgcatta ccttcattgg tcaccaggca taccgaacct gtctctgcca ccgcgaaatt
     2341 acagcccgta atacctattt cagcactgag gaaatcttcg cggatttttt gccggatgaa
     2401 taaggtcatc gcttcaggcg tttccggccc ctcatagccc agacgttcgt gtagcactcg
     2461 acggatctga tggcgatctt tatgaattgc cgggaccaca acatgagagg gcggatcttg
     2521 atccagctgg agaatgtatt cacccagatc ggtttcaatc acctgaatgc cagcatcctg
     2581 caacacatga ttgacaccaa tctcttcggt caccatcgat ttagatttca ccaccttccg
     2641 ggcatttttg cgttgggcaa cctgtaaaat gtagcgagtg gcgtcttctt tagtttttgc
     2701 aaaatagacg tgaccgccgt tttgcgtcac tttttctgag agctggtaca gataagcgtc
     2761 gagattactt agaacatgat cacgtatctg ggcggcccga tcgcgccact cctcccagtg
     2821 ccccaattca tcgaccattt tttgccgatt tgccccaata cgctgctgcg cgtttgccac
     2881 cgctttgcgc atgatcggat cttcaatttg ctgacggatg cgtgtcttaa aatctgtatt
     2941 actggttttg atcgacatct ttatatcctc agcggctcat caacacttca gcaatatgca
     3001 tcactttgac tttctgccct tcccgttgta atcgcccact gatgttcagc aggcaactca
     3061 cgtcagcacc aattaaatac tcagggcgga cttccatcag gtgcgcaacc ttttctttca
     3121 ccatctcgcc ggatatttcg gccattttga ccgagaacgt gccgccaaat ccgcagcagg
     3181 tatcctgttc agcaaaggtc aacagctcca gtccacgcac atttttcagc agcgtaagtg
     3241 gctcgtcctt cactcccagc ttacgggcca ggctacaaga tgggtgatac accgctctcc
     3301 cttgcaaact ggcacctaca tcgactaccc ctaatttatt aacaataaaa gaggtgagat
     3361 cctgcatacg cgcggcaacc tttgcggcac gtgatgccca ttcaggttca tccgccagat
     3421 acatcgggta actttttacg gcataggtgc aggagccagc cggtgaaata atgggatcat
     3481 cgttatcctc cagtgcggcg atcagatttt tcatccctgg aatcgcttct ttgatataac
     3541 cgctattgat cgcaggctga ccgcagcatc cctgtttctc cgggaaattt acgcgacagc
     3601 cgagtttttc cagtagcagc acggagtctc gtgccattct tgatttcagg gcgtcaccaa
     3661 tacaggtgac aaagaaattg acattcacaa ctaatactcc attacttcat gcccatttat
     3721 gcccgatgtt tcccccccac cgggggatca atcatcaata aatatgacgt tcttttttaa
     3781 ttggctagca acataccacc cctcatttgt atgacaaatg ataatctttc atccaaacgt
     3841 caaatgcaat aactaatttt atttctttcg gcatcgctca cattttatgg ggaaatcgac
     3901 gatcattttt ggcataagaa atccatttaa aaatggctac atcccttata cctattcaga
     3961 gctacttatt caggggttat tgagatatac actgatggtg tacaaaaaaa tcaccgacaa
     4021 taatcacgta tcgagtaata gtcgggattt tatttaattg aagcaattta gtttgcattt
     4081 ttatttaaaa actgagctag agtccatcag aaaaaaaata tataaagccc aaaccaccgt
     4141 tggaaataga aatagttgaa aggctcacaa ctcccggaat gcggattatc tttgccctga
     4201 tttctggcgg ttttattcag ggtgcaagct gtctgaccct ttcccgatat tctcccggag
     4261 tacaaccaaa ctcgcggaca aacgccttgt gaaaagatga ttcactggca tagcctactg
     4321 actcagcgat caccacaaca gggagcattt cccgggaaaa catctgggcc gctagttgta
     4381 gacgcaactt tgttaataca gccagcggcg tggttccgga aacatcacgg aaaagctggg
     4441 caaaacttgc ccgggacatg tgggcgatgc tggccagcga ttcgacggtc caggcgtgtc
     4501 ctggcatttc cagcatttgc tgtattaccg caccaagacg tggatgcaga agcaaactga
     4561 gaatgttttt ctctgtatta acctgtgcaa tccactcacg caccgcgagg gtaaagaatg
     4621 tagcgcagat ctggctacac agtgcatcca ctcccggcat tgccaatctg gattcctgtt
     4681 gtaaaaacgg gatcgcctcc ctcagccagt tatattctac gctgtggtta accggtgcta
     4741 aaaacagcgt ttccggcaaa gacgttaaaa aataacgcgc cgaatgttgc agccgaagag
     4801 tgccgcagac aatacaggtt gattcattat cgacatgact caggcgatgg gctgaatttt
     4861 gtggtagcag gaccacattt cccgggcgta atgtaaaaat ctcccccgtc ggcatttcca
     4921 gcttcgccgc tccttgcgtt aacgcatgcc aacgaataac cgataattcc ccggcaccat
     4981 gtggaagctg ccagtcactt cctaacacgc aattcttatc gatcgttcct tgtggagcgt
     5041 taagcatcaa caaacggcta agggcatcca tatcagactc ctgagtggtt tttgatta
//
LOCUS       DAZUCE010000294         5071 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZUCE010000294 DAZUCE010000000
VERSION     DAZUCE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.295"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..2038)
                     /locus_tag="ACHY03_004692"
     CDS             complement(<1..2038)
                     /locus_tag="ACHY03_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS3907838.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAVTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNASASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAAQSASAAADSKTAAASSASAASTNAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKAAYDNAAKRLQKDQNGAD
                     IPDKGRFLSNINVYSKGEVDQKKGMRYVVVNAPAGVQEGKYYPLVIKRNDSHRASRVV
                     ISTPSRTANHRMNNCEFNGFVCAGGWTDRGSYACGMFWAYSSSERAIHSILMSNKGDT
                     VDSVFYIEGGAFPVEVFLEEGLSVTAPASDYVVAETTYKFGATDPYSESVAVNLI"
     gene            complement(2073..3286)
                     /locus_tag="ACHY03_004693"
     CDS             complement(join(2073..2992,2992..3286))
                     /locus_tag="ACHY03_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc31 family transposase"
                     /protein_id="HFS3907839.1"
                     /translation="MISSPQHKTGDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGIAPSATPITPDQQRIRELEKQVRRLEEQNTIFKKG
                     YRALDVRLAERFTIVARLSDSHSVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSE
                     IRRAWNQSRGSAGARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQE
                     HTCLPNLLERQFTVPEPDRVWCGDITYIWAGNRWCYLAVVMDLFARRVIGWSLSANAD
                     TALISSALRMAYEVRGQPRDVMFHSDQGSQYTGLKYQQLLWRYRIKQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPTDGYTGKDVARQQISSYILNYYNSVRPHHYNGGLTPEESENR
                     YHFYCKTVASIT"
     gene            complement(3326..4699)
                     /locus_tag="ACHY03_004694"
                     /pseudo
     CDS             complement(3326..4699)
                     /locus_tag="ACHY03_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            complement(4674..>5071)
                     /locus_tag="ACHY03_004695"
     CDS             complement(4674..>5071)
                     /locus_tag="ACHY03_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFS3907840.1"
                     /translation="AERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQA
                     DAQELKNARDSAEVVETAFCTFVLSRIAGEIASILDGLPLSVQRRFPELENRHVDFLK
                     RDIIKAMNKAAALDELIPGLLSEYIEQSG"
BASE COUNT         1010 a         1455 c         1286 g         1320 t
ORIGIN      
        1 aaattaaatt taccgcaact gattcagaat aaggatcagt tgcgccaaat ttataagttg
       61 tttcagcaac aacataatca gaagcaggtg cagtaacgga gagtccttct tctaaaaaaa
      121 cctctacggg aaatgcccca ccttctatat agaatacgct gtctacggta tcgcctttat
      181 tactcatcag aatggaatga atagcgcgtt ccgatgatga ataagcccag aacataccac
      241 aggcatagct accccggtcc gtccagccgc ctgcgcaaac aaaaccatta aactcacagt
      301 tattcattct gtgattagct gtgcgacttg gcgttgaaat gacaacgcgg gatgcccgat
      361 ggctgtcatt cctttttatc actaacggat aatatttacc ttcctggaca cccgccgggg
      421 catttaccac aacgtatcgc atcccctttt tctgatccac ttcacctttg ctgtaaacat
      481 taatgttact caggaagcgt cccttgtcgg gaatatcagc accgttctgg tctttctgca
      541 gacgtttcgc tgcattgtca taggctgctt ttactgcctt tggcgttgcc gccagcgttt
      601 cagacgtgct gttggtcgca ctgctgagct gtactatccc ctttttcgtc gtgctcgcat
      661 cctcaagcgc cacggcggat gcaatatcct ctgcccgttt tgctgctgtc tcggcgcgcg
      721 ttgccgcgga ttccgccgta cttttgctct gagctgccgc cgtcgcactg ccagctgcct
      781 ctgtcgcctt cgtggatgcc gtcgtggcgc tgcccttcgc tgctgacgct tgtctggtcg
      841 cctcatcttt tgaagcagac gccgatgatg ccgatgacgc cgccgaactg gctgacgatg
      901 cggcagccgt ttttgaggat tctgcgctgg tttccgacgc tttcgcgttc gtttcggatg
      961 tcttcgctgc ggaagctgac ctcgctgctg ccgtggcctg ttcagtggct tcgccagcct
     1021 tcgttgtggc tgttgaagcg gatgatgcgg cgctttctgc cgactttccg gcagcggtgg
     1081 cactggctga ggcctgcccg gcatttgttg acgcggcgct ggcagatgag gcagccgctg
     1141 tttttgagtc tgctgctgcg gaggcactct gtgctgctgc cgtctcagaa gacttagcgt
     1201 tcgtctcgga cgtttttgcc gccttcgcgg aattgcctgc cgctgttgcc gaggaggctg
     1261 cactactggc gctcgaggct gcgctcgttt ctgatgattt tgccgcctct tttgaagccg
     1321 acgcatctct ggctgaggtg gcagcttctg acgctttcgt ggtcgcggtg gatgcagaag
     1381 tggcggctga ttgttgtgac gctgaagcat tcgtttctga cgttttcgcc gcaccggcac
     1441 tggtggccgc cgcgcttttt gaggactctg cagcggcagc actttttgat gcttcagtgg
     1501 cctttgttga tgccgttact gcgctagaag acgctgactg agccgacgac gcggcctgtc
     1561 cggctgacgt gctggctgcg cgtgctgagt ccgcagcatc agtcgcatgg gttgccgcct
     1621 cacgggctga tgtgctggca tcgctggctg acttcttcgc ggctgccgtg ttctgtgcca
     1681 ccgcggacgc gttacgcgcc acctcttcca ccatcagctc aaaacggcgc agtgcctccg
     1741 gacggacatc atcctcagtc atggcaccaa gaaaatcatt cagcgtaccg ggttgagaat
     1801 cttcatacac ggtgatggtc ccggcatgtg acggcgggaa cccttccacc aacagaataa
     1861 cgctgtactg accgtactca acgtccatgc tgtaacgccc ggcttcatcc ggattttctg
     1921 aggccagcgt gttcaccacc accgtggtgc tgttacgttt tgctttcagc tggattgtgc
     1981 agttctgtac cggttttcct gtgccgtctt tcagtacacc tgaaatcttt actgccatat
     2041 tcaccccaca aaaaagcccg cctgtgtagt ggtcaagtaa tactggccac ggttttacag
     2101 taaaaatggt atctgttctc tgactcttcc ggcgtcagcc ctccgttata atggtgaggc
     2161 ctgacgctat tgtaataatt cagaatataa ctgctgattt gctgccgggc cacgtctttg
     2221 cctgtgtagc catcggttgg cacccattct gttttcagac tgcggaagaa gcgttccatt
     2281 ggactgttat cccagcaatt tccccgtcgg ctgacacttt gctttatcct gtaacgccag
     2341 agaagttgtt gatatttcag tcctgtatat tgacttccct ggtcgctatg aaacatgacg
     2401 tcccgcggct gaccacgcac ctcatacgcc atccgcaggg cactgcttat cagggcagta
     2461 tcggcattcg ctgacaggct ccagccgata accctgcggg caaaaagatc catgacgacc
     2521 gccagatagc accagcgatt tcctgcccag atatacgtaa tatctccgca ccatacccta
     2581 tctggctcgg gcacagtgaa ctggcgctca agcagattcg gcaggcaggt atgttcctga
     2641 cgagcatttt tgtactgatg ttttccgggc tgacaactgc tcaggttcag atatttcatc
     2701 agacgcccgg cacggtaacg gctcatcggg acgccgtttt gggtcagcat ttcagccagc
     2761 gtgcgcgccc ccgcagagcc cctactttgg ttccacgccc ggcgtatttc gctgcacaac
     2821 ctgactcgcg ccggattaac cgtatcgcgt cgttttcgcc agtaccggta actgctgcgg
     2881 tgtatttcca gagcagaaca gaggctgaca actgagtggc tgtcactcag tctggcaact
     2941 atcgtgaacc gttcagcgag tcggacatca agagcgcggt agcctttttt aatatcgtat
     3001 tttgttcctc caggcggcga acctgctttt ccagttcgcg gatacgttgc tggtctggag
     3061 taataggtgt ggcagagggc gcaatcccct gacgctctcg cctgagctgg cgcacccaac
     3121 tctcaagcgt ggtagaaccg acattcatcg cttcactggc ttgtcgatat gagtagccct
     3181 tatcaacaat tagctgtgca cattccagcc tgaattcagg ggtgaaagta cgtttggttt
     3241 tcttgttcat taagtcacct gttttgtgtt gtggtgagga tatcaccttt aatcaggtgg
     3301 ccaaatttac tgtgccacta catatcgaca tttctgcacc attccggcgg gtataggttt
     3361 tattgatggc ctcatccaca cgcagcagcg tctgttcatc gtcgtggcgg cccataataa
     3421 tctgccggtc aatcagccag ctttcctcac ccggccccca tccccatacg cgcatttcgt
     3481 agcggtccag ctgggagtcg ataccggcgg tcaggtaagc cacactgtca ggaacgggcg
     3541 ctgaataatg ctctttccgc tctgccatca cttcagcatc cggacgttcg ccaattttcg
     3601 cctcccacgt ctcaccgagc gtggtgttta cgaaggtttt acgttttccc gtatcccctt
     3661 tcgttttcat ccagtctttg acaatctgca cccaggtggt gaacgggctg tacgctgtcc
     3721 agatgtgaaa ggtcacactg tcaggtggct caatctcttc accggatgac gaaaaccaga
     3781 gaatgccatc acgggtccag atcccggtct tttcgcagat ataacgggca tcagtaaagt
     3841 caagctcctg ctggcggatg acgcaggcat tatgctcgca gagataaaac acgctggagg
     3901 ggtcatccgg cgtccatttg aggccaaacg gcgtctcttt gtcgccaaat ttaagatact
     3961 gctcctcccc gcaatgcggg caggcaacat gaaaacgcat aaaatgcggg gattcactgg
     4021 ctgcacgctc aatctgacag gtgcctctca cttttggcgt ggagccacgg atggactttg
     4081 gccagaccga gccttcaata cgcttgtcac ccaggaacgt cggagagcct tcctgttcaa
     4141 tatcatcatc aaaagcagca agttcatcat aacccgccac atccaccgac ttttcacggt
     4201 agttttttgc cgctttaccg cccaggcacc agaagccacg cccattagtg aaacgcttca
     4261 tggtgagcgt gttatcccgg tgctttttgc cataccacgg ggccagcgcc agcagcgacg
     4321 gaatatcacg aatagtcggc tcaacgtggg ttttcataaa gttctcggca tcaccatccg
     4381 tcggcaacca gataagggtg ttgcgctgct tatgctctat aaagtaggca taaacaccca
     4441 gcagcatttt ggaataaccg acacgggcag acttcaccac attcacctca cggatgtagt
     4501 cgctgcccat cgcattcatg atggcccgct gaaagggcag tgtttcccag cgcccttcct
     4561 ggtatgcgga ttctttcggg agatagtaat tagcatccgc ccattcaacg gcggtctgtg
     4621 gctccggcct gaacagtgag cgaagcccgg cgcggacaaa atgccgcagc ctgttaacct
     4681 gactgttcga tatattcact cagcaacccc ggtatcagtt catccagcgc ggctgctttg
     4741 ttcatggctt tgatgatatc ccgtttcagg aaatcaacat gtcggttttc cagttccgga
     4801 aaacgccgct gcaccgacag ggggagcccg tcgagaatac tggcaatttc acctgcgatc
     4861 cgcgacaaca cgaaagtaca gaatgcggtt tccaccactt cagcggagtc tctggcattc
     4921 ttcagttcct gtgcgtcggc ctgcgcacgc gtaagtcgat ggcgttcgta ctcaatagtt
     4981 cctggctgga gatctgcctc gctggcctgc cgcagttctt caacctcccg gcgcagcttt
     5041 tcgttctcaa tttcagcatc cctttcggca t
//
LOCUS       DAZUCE010000295         5062 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZUCE010000295 DAZUCE010000000
VERSION     DAZUCE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.296"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(1628..1822)
                     /locus_tag="ACHY03_004696"
     CDS             complement(1628..1822)
                     /locus_tag="ACHY03_004696"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFS3907841.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIGWCIARAEYCKKTGRF"
     gene            complement(2080..2547)
                     /locus_tag="ACHY03_004697"
     CDS             complement(2080..2547)
                     /locus_tag="ACHY03_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907842.1"
                     /translation="MRDKTREAMRFFLGGRCYTAENLEKDYLAEVANYSDDRWEAPQR
                     AARLAAAVKRYKTSEMLRFIFATTAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVF
                     GEKGRQHRSADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRIYQARIKAS"
     gene            2707..2961
                     /locus_tag="ACHY03_004698"
     CDS             2707..2961
                     /locus_tag="ACHY03_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HFS3907843.1"
                     /translation="MKITTLSSRELNQDVTRAKRATNYGPVFITDRGKTAHVLLSIEE
                     YRKLTQQRRTLIDILAMPDADAVEFEPPRITVSLKPAEFL"
     gene            2961..3377
                     /locus_tag="ACHY03_004699"
     CDS             2961..3377
                     /locus_tag="ACHY03_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFS3907844.1"
                     /translation="MYILDTNVISELRKARTGRIDNNVAKWALHTDAADFFISVISIM
                     ELEQGILQVERRDMRQGIMLRSWMDQHVLPAFSDKILPVDTAVALRCSRLHVPDRRSE
                     RDALIAATALVHGMTVVTRNVGDFELTGVSIINPWA"
     gene            4275..4637
                     /locus_tag="ACHY03_004700"
                     /pseudo
     CDS             4275..4637
                     /locus_tag="ACHY03_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            4641..4996
                     /locus_tag="ACHY03_004701"
                     /pseudo
     CDS             4641..4996
                     /locus_tag="ACHY03_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
BASE COUNT         1318 a         1206 c         1189 g         1349 t
ORIGIN      
        1 aaaaaagttg gttttgcgtg gggggtgggc atccggggca tgagaacaga ctcgcgtttt
       61 tctggagaaa ctgcggggat ttttgattaa acaatactca ccgcagagcg gaattttatg
      121 caacgccggc tgtgcggcac agggattttt aatccccagc atgtcagagg gtgcggatga
      181 cggttcccgt ctggcgtttt catgggcgcc ggttgtcaaa aaataccagc gtgatcggtc
      241 gaaggataag cgcagattaa tcagtgatga ttgagcgcga atctgacaac aagtatagcg
      301 acacagcaac aaaaaagacg ccagaaacgc gctcagaggc tttgtaacgc gattttttac
      361 atgaggcaat ggtttcgctt tgaggcgttc agccgtgttc agggcgcgat tctggtacat
      421 acctgactgt aatcatccct acgaactact ctttgcacca gaaacgcgca ggagtgtttt
      481 agcgtgtctc cggcgtgatg tcgagttgtg cggtaatgag tgagcgaaaa aaatcccctg
      541 gattacaggg ggaacccatg atcagacttt gaagtttatt tgttatttga tggaatgagc
      601 acgaatcccg gagatgacgt tcaggtggca ggaaacgcgc tcagaagctt tctaacgcac
      661 ttttttgcat ctggcaatgg gtttcatatg ttttggtggg ttcaggctgt cagagacgtt
      721 ttgagcgcgt ttctggcgct tgctgctact gccaaaccat ccccatggcc agttgatgcc
      781 agaaacgcgc aggaattttg tagcgcgtct ccggtccctg atcaatgaat tccctgtcat
      841 taacatgcgt ttacccggaa acgcgccctg atgttctatc gtatcgcggg aacggtgtcg
      901 agggtgtcgg ggcaacgccc tgaccaggtg gtaatcgtat agccgtgcgt gcgcggttat
      961 acgattacac atcctgtccc ggaatcagga ttgtttttcg cgcacaactg cggcaaattc
     1021 atcccgttgc ggttgcaacg atgggtatat caaaactacg aaaattattc aggcatttgc
     1081 tacccccctt gccctggcct ccaccagtca cgggggattt tttcgcctgt cctgattgtc
     1141 ttttgccgat ggttacggca gattggaaaa gagcgcattt gtggtgctct ctccaaaata
     1201 atatttctca ttactcaaac tctgatggct tccccctgta atccaggggg attttttaat
     1261 ttatcgctat gcttcgtaat tcattcagaa tacagacagt atggttaatc agtttttcat
     1321 cattttttcg gtgtttgccc tgtcctgcat cagaacgcta aaaaaacagg attggaatct
     1381 ctttatgacg aatcctccat ttcaggattt tttatgtaac gtctactaca tgcgatccgt
     1441 tggtgaaccg ttagttatat attggttata tgttagttat atcttagtta tacttttgct
     1501 gcgcgtcagt tacagaggcg ttttgagggt aaattctgga ggcacgtgac cgactgtata
     1561 caaaatgctg gcgcattttg atacgtcgac gtgccaaagg ggacgcccct ttgaaacccc
     1621 gccagaatta aaagcggcct gtctttttac agtattcagc tctggctata caccaaccta
     1681 tattttctcc tttggcataa tgaagcgcat cattccattt tttccatgct ccaatgtaat
     1741 caccttcacg ctccagtctg acagcttcgt ttgcgggtga aaaatattta ttttctaaat
     1801 aaattgcttt gtcctgattc atattcgtaa tttatatttt tatttgaatt aaaaaagagc
     1861 gagcccatcg ggttaataca tattggcatg actgttgata tatcagtcca acagcacgct
     1921 cgtaatcgtt ttaacgtgat tttcgcaaca tggcaatccg ggttcatctg tccggtaatc
     1981 aataaaaacc gtgcgaaaaa cgggacagga tgtggccatt aatgctaatg tatgcataca
     2041 ttagcattaa tggccctgac aaataccagg catccggtct caggatgcct tgattcttgc
     2101 ctggtagatg cgctttaccc gttcaatttc tgccagcgtt gcggaccagt gtagttcggc
     2161 ggcatgacga tatctggcag ccattttttc caccatctcc ggattgctgt ccgcgctgcg
     2221 gtgctgacgc cccttctccc caaaaacttc aacaaccagc cactggcttc ctgttctgcc
     2281 gaacaatgcc ttgcacaaac gtctcacagt cagcggggtt aaatctggat cgggatcgta
     2341 ggcagtggta gcaaaaataa agcgcagcat ttctgatgtc ttgtaacgct ttacggcagc
     2401 tgccagacgt gcggcgcgtt ggggtgcttc ccagcggtcg tcactgtaat tagcaacttc
     2461 agccagataa tccttttcaa ggttttcagc cgtgtaacac ctgccgccta aaaaaaaccg
     2521 catggcctcc ctggttttgt ctctcacata tcatccctca ttaatgctaa tgtatgcata
     2581 cattagcatt aatatctgac agcaacaagc ggcgaagact acccctaatc aagtacattt
     2641 cttgcattgt actatttcat gccctacaat gtagtaacgt acctatatac atcaaaaggc
     2701 aacagcatga agattacaac cctatccagt cgggaactta atcaagatgt aactcgagcc
     2761 aaacgcgcca cgaattatgg tccagtattt attactgatc ggggtaaaac cgcacatgta
     2821 cttcttagta tcgaagaata ccgaaaattg acgcaacaac gacgaactct cattgacatt
     2881 ctggcgatgc ccgatgctga tgcagttgaa tttgaaccgc ctcgtattac ggttagttta
     2941 aaacctgcgg agtttttata gtgtatattc ttgatactaa tgttatctct gagctacgga
     3001 aggctcggac aggacgtata gataataatg ttgcaaaatg ggcattgcat acagatgcgg
     3061 cagatttctt tatttctgtt atttccataa tggaacttga acagggaata ttacaagttg
     3121 aacgtcgaga tatgcgacaa gggattatgc tacgttcttg gatggatcag catgtgttac
     3181 cagcgttttc tgataagata cttcctgtag atactgcggt tgcattacgt tgttctcgtt
     3241 tacatgttcc cgacaggcgc agtgaacgag acgcattgat cgcagcaacg gcgctggtac
     3301 atgggatgac ggttgtaacc agaaatgttg gtgattttga attaaccgga gtatcaatta
     3361 ttaatccctg ggcttgatat cgcttccctt cttctagggg tccgcttgga aaacggaaat
     3421 tttcccacgc taagagaatt gagttgaacg agtggttaca tccaagccag ctaactcact
     3481 gaacgtaaag gctgtacgaa atgttccgac cgtccggatt cctgacagtc atttggctaa
     3541 aaaatagtct taacgtggtt tattttccga attccaggct gaccccataa aagcatcctt
     3601 cagttcagcg tcgattctta aactcatatt aacctgtgcc attcatcacc tcgtcattac
     3661 cacgtatata cacagtatat aattattcag atatcgtcac catgatatgc cgcgccagca
     3721 acatggaagg cggcactcca tgctgtatat ggcagaggag taaaaccgcc gaatcccggc
     3781 gtaagccggt acagtcgtcc gaatgtcagt agttctactc ccccctgcga ggccagtgct
     3841 gacgcgggac atggaacggg tgctggagcg aagtggggaa gctccggtat actggccaaa
     3901 cctacacgcc tccttccgtc tccgggaaga tgctggcttc gtaccctgcg tgcgtctgat
     3961 ctgtagctgg ccgtggcacg gctacggaca gattctgctc cgcaacagta tcggcgtgcc
     4021 tgacctcctg aaagtcccgt aacgcatttt ctcacctccc atgccctgac gctattgagc
     4081 gcgttacaga acaatttaag tcatttaacg agcctccaac ggctcgatcg aaataaatca
     4141 aatcgtaatc gtcaacgata tatgagagga gaccccgctc gccgcaaatc gatgaccgaa
     4201 cgtagtgagc gaatcgacga ggaagcggaa gagaacctgt acgaaaatgg agcaattacg
     4261 ccccaccgtc aagaaatgag ctggctctcc acggcgcgcc atcatagaca gatgtggctc
     4321 tgcaactgcg ccatgcgatc ggagaccgac cagtaattat ctttaatgcc cggttcgaca
     4381 cccgcattct gaaacagact gctgccgcac atagcgatcc ggctgactgg ctggaagaac
     4441 tgacagtata ttgtgcgatg gaactggcag caggatatta tggggccacc aaccgctatg
     4501 gcacgatttc tctggcctgt gctgccagca ggccggactg acatgggaag ggctggcaca
     4561 ctcagctgat cgccgacaca cggatgacgt caggggtggt aaacgctatt actgcatatc
     4621 atcaggaatt gctgcagctt atgtactgcg ataaccctga actcaactat gcaaacctga
     4681 aatccctgca aaaacagctg gatgaaatgt atacctgcat ccgtaataat cagatgatgc
     4741 tcccaaacta cggaaaaatg cgccggtatg gtgaaccggt ttcaacaacg tttgtagagt
     4801 cgacaatcaa cgaagtgatc gccagacgat gacccaaaag caacagatgc agtggggtaa
     4861 ggaaggtacg cattatttgt tacagaccag aaccgctgtc ctgaataatt aactgcagga
     4921 caaattcgcc agttggtacc cgggttgcac gatagacgct cagagtggag gaaagttatc
     4981 cgccatggca gcataactac ttccccagtt ttttcagctc tcccttacca atatcctacg
     5041 acgatcacat cgaaaaaaaa ac
//
LOCUS       DAZUCE010000296         5020 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZUCE010000296 DAZUCE010000000
VERSION     DAZUCE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.298"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            263..4516
                     /gene="fdeC"
                     /locus_tag="ACHY03_004702"
     CDS             263..4516
                     /gene="fdeC"
                     /locus_tag="ACHY03_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFS3907845.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPVYRPGKNNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRSLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAEGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQNAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLALSASLTGTASEGATVSSWTEKGNGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGSWTEKGNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VIGNYALADGKAKQTYKVTVTDANNNLLKDSDVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSADNPVGGNMWVDIQTPEGVTEEDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKGEEAMK"
     gene            complement(4637..>5020)
                     /locus_tag="ACHY03_004703"
     CDS             complement(4637..>5020)
                     /locus_tag="ACHY03_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFS3907846.1"
                     /translation="KLRRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVC
                     KQTDSEMPKEWTINRGLYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERF
                     YYNENFVEIDSNNIVCEIFIPVRPV"
BASE COUNT         1341 a         1161 c         1370 g         1148 t
ORIGIN      
        1 gaaatataac ttaagtatct atttaatgac ttgcacaaaa agctaaattt tcacccataa
       61 ataaaaatat aatcccgcgc ccaaccacct gatgagtggc tacaggcact ggatatatta
      121 ggtggcggtg tactttctta cataaaggta tttccttttc tgcggaaaag gaaaccggga
      181 aatccccggt ttttctgaca agcagacgcc attatttgtg tctgcctatg ttcgttaatt
      241 cgttcatcag gaaattatct caatgtcacg ttataaaaca ggtcataaac aaccacgatt
      301 tcgttattca gttctggccc gctgcgtggc gtgggcaaat atctctgttc aggttctttt
      361 tccactcgct gtcaccttta ccccagtaat ggcggcacgt gcgcagcatg cggttcagcc
      421 acggttgagc atgggaaata ctacggtaac tgctgataat aacgtggaga aaaatgtcgc
      481 gtcgtttgcc gcaaatgccg ggacattttt aagcagtcag ccagatagcg atgcgacacg
      541 taactttatt accggaatgg ccaccgctaa agctaaccag gaaatacagg agtggctcgg
      601 gaaatatggt acagcgcgcg tcaaactgaa tgtcgataaa gatttctcgc tgaaggattc
      661 ttcgctggaa atgctttatc cgatttatga tacgccgaca aatatgttgt tcactcaggg
      721 ggcaatacat cgtacagacg atcgtactca gtcaaatatt ggttttggct ggcgtcattt
      781 ttcaggaaat gactggatgg cgggggtgaa cacctttatc gaccatgatt tatcccgtag
      841 tcatacccgc attggtgttg gtgcggaata ctggcgcgat tatctgaaac tgagcgccaa
      901 tggttatatt cgggcttctg gctggaaaaa atcgccggat attgaggatt atcaggaacg
      961 cccggcgaat ggttgggata tccgcgcaga gggctattta cctgcctggc cgcagcttgg
     1021 cgcaagcctg atgtatgaac agtattatgg cgatgaagtc gggctgtttg gtaaagataa
     1081 gcgccagaaa gacccgcatg cgattaccgc tgaagtgaat tacacgccag tacctcttct
     1141 gaccctgagt gccgggcata agcagggcaa gagtggtgag aatgacactc gctttggcct
     1201 ggaagttaat tatcggattg gcgaacctct ggagaaacaa ctcgatacag acagcattcg
     1261 cgagcgtcga atgctggcag gcagccgcta tgacctggtt gagcgtaata acaacatcgt
     1321 tcttgagtac cgcaaatctg aagtgatccg tattgctctg cctgagcgta ttgaaggtaa
     1381 gggcggtcag acactttccc tggggcttgt ggtcagcaaa gcaactcacg gactgaaaaa
     1441 tgtgcagtgg gaagcgccgt cattactggc tgaaggtggc aaaattaccg gtcagggtag
     1501 tcagtggcaa gtaacgctcc cggtttatcg tccaggtaaa aacaattatt atgcgatttc
     1561 tgcggttgcc tacgataaca aaggcaatgc ctcaaaacgc gtgcagacag aggtggtcat
     1621 taccggagca ggtatgagcg ccgatcgcac ggcgttaacg cttgacggtc agagccgtat
     1681 tcaaatgctt gctaacggta atgagcaaaa accgctggtg ctgtctctgc gcgacgccga
     1741 gggccagcca gtcacgggca tgaaagatca gatcaagact gaactaactt tcaaaccggc
     1801 tggaaatatt gtgactcgtt ccctgaaggc cactaaatca caggcaaagc caacactggg
     1861 tgagttcacc gaaactgaag caggggtgta tcagtctgtc tttactaccg gaacgcagtc
     1921 aggtgaggca acgattactg ttagcgttga tggcatgagc aaaaccgtca ctgcagaact
     1981 gcgggccacg atgatggatg tggcaaactc caccctgagc gctaacgagc cgtcaggtga
     2041 cgtggttgct gatggtcagc aagcctatac gttgacgttg actgcggtgg actccgaggg
     2101 taatccggtg acgggagaag ccagccgctt gcgatttgtt ccgcaagaca ctaatggtgt
     2161 aaccgttggt gccatttcgg aaataaaacc aggcgtttac agcgccacgg tttcttcgac
     2221 ccgtgccgga aacgttgttg tgcgtgcttt cagcgagcag tatcagctgg gcacattaca
     2281 acaaacgctg aagtttgttg ccgggccgct tgatgcagca cattcgtcca tcaccctgaa
     2341 tcctgataaa ccggtggttg ggggtacagt tacggcaatc tggacggtaa aagatgccta
     2401 tgacaaccct gtgaccagcc tcacgccgga agcgccgtca ttagcgggtg ccgctgctga
     2461 aggttctacg gcatcgggct ggacaaataa tggtgatggg acgtggactg cgcagattac
     2521 tctcggctct acggcgggtg aattagaagt tatgccgaag ctaaatggac agaatgcggc
     2581 agcaaatgcg gcaaaagtaa ccgtggtggc tgatgcgtta tcttcaaacc agtcgaaagt
     2641 ctctgtcgca gaagatcacg taaaagccgg cgaaagcaca accgtgacgc tggtggcgaa
     2701 agatgcgcat ggcaacgcta tcagtggtct tgcgttgtcg gcaagtttga cggggaccgc
     2761 ctctgaaggg gcgaccgttt ccagttggac cgaaaaaggt aacggttcct atgttgctac
     2821 gttgactaca ggtggaaaga cgggcgagct tcgcgtcatg cctctcttca acggccagcc
     2881 agcagccacc gaagccgcgc agttgacggt catcgccgga gagatgtcat cagcgaactc
     2941 tacgcttgtt gcggacaata aggctccgac cgtcaaaacg acgacggaac tcaccttcac
     3001 cgtgaaggat gcgtacggga acccggtcac cgggctgaag ccagatgcac cagtgtttag
     3061 cggtgccgcc agcacgggga gtgagcgtcc ttcagcagga agctggacag agaaaggtaa
     3121 tggggtctac gtggcgacct taacgctggg atctgccgcg ggtcagttgt ctgtgatgcc
     3181 gcgagtgaac ggccaaaatg ccgttgctca gccactggtg ctgaatgttg ctggtgacgc
     3241 atctaaggct gagattcgtg atatgacagt gaaggttaat aaccaactgg ctaatggaca
     3301 gtctgctaac cagataaccc tgaccgtcgt ggacagctat ggtaacccgt tgcaggggca
     3361 agaagttacg ctgactttac cgcagggtgt gaccagcaag acggggaata cagtaacaac
     3421 caatgcggca gggaaagtgg acattgagct tatgtcaacg gttgcgggag aacacaatat
     3481 ttccgcttcg gtgaatggtg ctcagaagac ggtcacggtg aaattcaacg cggatgccag
     3541 caccggtcag gcaaacctgc aggtagacac cgccgttcaa aaagtggcaa acggcaaaga
     3601 tgcctttacg ctgacggcga ccgttgagga taaaaatggt aaccctgttc cagggagcct
     3661 ggtgaccttt aatctgcccc ggggtgtcaa gccgcttaca ggcgataatg tctgggtgaa
     3721 agccaacgat gaggggaaag cagagttgca ggtggtttca gtgactgccg gaacgtatga
     3781 gatcacggca tcggcaggga atagccagcc ttcgaatacg cagactataa cgtttgtagc
     3841 cgataaggct accgcaaccg tctccggtat tgaggtaatt ggcaactatg cgctggcgga
     3901 cggcaaagcc aaacaaacgt ataaagttac ggtgactgat gccaataaca acctgttgaa
     3961 agatagcgac gtgacgctga ctgccagccc ggcttcgtta aacctggaac cgaatggcac
     4021 tgcgacaacg aatgagcaag ggcaggctat tttcaccgct actactactg ttgcggcgac
     4081 atacacactc aaggcgcaag tgagccagac caacggtcag gtatcaacga aaactgccga
     4141 atctaaattc gttgcggatg ataaaaatgc ggtactcacc gcatcatctg atatgcaatc
     4201 tctggtggcg gatgggaaat cgactgcgaa gctggaggtg acactgatgt cggcagataa
     4261 ccccgttggc gggaatatgt gggtcgacat tcagacgccg gaaggggtga cggaggagga
     4321 ttatcagttc ctgccgtcga aaaatgacca tttcgtgagc ggaaaaatca cgcgtaaatt
     4381 tagtaccagc aagccaggtg tctatacgtt tacatttaac gccctgacgt atggcgggta
     4441 cgaaatgaag ccagtgacgg tgaccattac cgcggtggat gccgatacgg caaagggcga
     4501 ggaggcgatg aaataatatt aagaatatga aagtaatatt ctaataaaca ataatggcgt
     4561 tgacatcttc aacgccatta tttattgaat tgcctgacag tagtctcgtt ataagttcat
     4621 ttagatatag cgacagctaa accggacgaa cggggataaa aatttcgcaa acaatgttat
     4681 tgctatcaat ttcgacgaag ttttcattgt aataaaaccg ctcaatatca ctgccgttga
     4741 ctctggctaa ccccctggcg ggtaattcca tcagatagag gtttcgcgcc cattcagggt
     4801 agtgctccca ctcaccttca tagcggaaag aggcatacag tcctcggtta attgtccact
     4861 ctttcggcat ctctgaatcg gtttgtttgc ataccgtacc agcaaaagtt tcaaccgtga
     4921 gcaaatcgac cgattttgtt gacggttcaa acgtggagac gaaatagata tccgtcttaa
     4981 aattattcat acatctggct aaatgatgac gtcgcagttt
//
LOCUS       DAZUCE010000297         5004 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZUCE010000297 DAZUCE010000000
VERSION     DAZUCE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.299"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            28..1380
                     /gene="celF"
                     /locus_tag="ACHY03_004704"
                     /gene_synonym="chbF"
     CDS             28..1380
                     /gene="celF"
                     /locus_tag="ACHY03_004704"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFS3907847.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1393..2151
                     /gene="chbG"
                     /locus_tag="ACHY03_004705"
     CDS             1393..2151
                     /gene="chbG"
                     /locus_tag="ACHY03_004705"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFS3907848.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGVVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2198..4459)
                     /gene="katE"
                     /locus_tag="ACHY03_004706"
     CDS             complement(2198..4459)
                     /gene="katE"
                     /locus_tag="ACHY03_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFS3907849.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            4642..4905
                     /gene="cedA"
                     /locus_tag="ACHY03_004707"
     CDS             4642..4905
                     /gene="cedA"
                     /locus_tag="ACHY03_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFS3907850.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1215 a         1154 c         1332 g         1303 t
ORIGIN      
        1 cagtattctg tactgaaggg agaaattatg agccagaaat taaaagtcgt cactattggt
       61 ggcgggagca gctatacccc ggagttactg gaaggattta ttaagcgtta tcacgaattg
      121 ccggtcagcg aattatggct ggtggatgtc gaaggtggca aagctaaact ggatattatt
      181 ttcgatctct gccaacggat gattgataac gctggcgtcc cgatgaagct ttataaaacg
      241 ctggatcgcc gcgaagcatt gaaagatgct gatttcgtta ctacccaact gcgcgtaggc
      301 caattaccgg cgcgcgaact ggatgaacgt attccattaa gtcatggtta tcttggtcag
      361 gaaaccaacg gcgcgggcgg tctgtttaaa ggtctgcgta ccattccggt gatttttgac
      421 atcgtaaaag atgtcgaaga actttgtccg aatgcatggg tgattaactt cactaacccg
      481 gcgggaatgg tcactgaagc cgtttatcgt cataccggat ttaaacgctt tatcggcgtg
      541 tgtaatattc cgatcggcat gaagatgttt attcgcgatg ttctgatgct gaaagacagc
      601 gatgatttat ctatcgatct gttcggcctc aaccatatgg tgttcattaa ggatgtgctg
      661 gtaaatggca aatctcgctt tgccgaatta cttgatggtg tggcatccgg gcagttaaaa
      721 gcatctggcg ttaaaaatat tttcgatctg ccatttagcg aaggcttaat tcgttctctg
      781 aatctgttgc cgtgttctta tttgctttat tacttcaagc aaaaagagat gctggctatt
      841 gaaatgggcg aatactacaa aggcggcgca cgagcgcagg tagtacagaa agtcgagaaa
      901 caactttttg agctgtataa aaacccggag ttgaaagtta agccgaaaga actggaacag
      961 cgcggtgggg cttattactc tgatgcagcg tgcgaagtga tcaacgctat ctacaacgac
     1021 aagcaagcag aacattacgt taatatcccg catcatgggc atattgataa tattccggca
     1081 gactgggcgg tagaaatgac ctgtaagctg gggcgcgatg gcgcgacgcc acatccgcgc
     1141 attacgcatt tcgatgataa agtgatgggg ctgattcaca ccattaaagg cttcgagatt
     1201 gctgccagca acgccgcact tagcggagag tttaacgatg tgttactggc gctaaacctt
     1261 agtccgttgg tgcattccga tcgcgatgct gagctgctgg cacgcgagat gattctggcg
     1321 cacgagaaat ggctgccaaa ttttgccgac tgcatcgcag agcttaaaaa agcacattaa
     1381 ccgaggctga ttatggaacg cttactgatt gttaatgccg atgattttgg cttaagcaaa
     1441 ggtcagaact acggcattat cgaggcctgt cgcaatgggg ttgtcacatc gacgacggcg
     1501 ctggtgaatg ggcaggctat tgaccatgcg gtgcagttga gtcgtgatga accgagtctg
     1561 gccataggga tgcactttgt ccttactatg ggcaagccac tgacagctat gccggggtta
     1621 acccgcgatg gtgtgctggg aaaatggatc tggcagttgg cagaagaaga tgctttaccg
     1681 ctggaagaaa ttactcagga gcttgccagt cagtatttgc gtttcattga gctatttgga
     1741 cgcaaaccta cgcatcttga tagccatcat catgtgcata tgttcccgca gattttccct
     1801 attgttgcca ggtttgcggc tgaagagggg attgcgttgc gcatcgaccg tcagccgcta
     1861 tctaacgctg gtgatttacc ggctaatctg cgcagttcgc acggattcag tagcgcgttt
     1921 tatggtgaag agattagcga ggcactgttc ctgcaagtgc ttgatgattc cagccatcgc
     1981 ggggaacgct cgctggaagt catgtgccat ccggcgtttg tcgataatac gattcgccag
     2041 agtgcttact gtttcccacg gttaacggag ttggaagtgc tgacatcagc gtcgttgaaa
     2101 tatgcaattg ctgagcgtgg ttatcggttg ggtagttatc ttgatgtgta agtaaggtgg
     2161 taaggtcact ccccagggga cgtgacctta tccatattta cgccggaatt ttgtcaatct
     2221 taggaatgcg tgaccacacg cggtgtgctg ccatcagcgt taacagttca tccataaaac
     2281 taccgtcggc gctgtcagct tccacaatcc cttcttcacc ctggtcagcg accttgattg
     2341 ttgctttaaa cttgcgcgcg tctcccgcca gcgcaatcgg tttaaggtgt ttgtaggctt
     2401 ccatcaggta gtagttggca tcgccgttgt cagcgatatc cgcgatattg ccgcaaggga
     2461 caatgaccgc atcgaccgtc agcgaaggcg caccggcaaa ggtagcggct ataggcaaca
     2521 ccgtaccgtc atccgcagtc acttcaccca ttcgggagta gagcagtttg gcatgaacgc
     2581 ctttggcctt cagggccttg agaatggcca gaaggtctgc cgatctcact tcatcattaa
     2641 gtaaaatcgc taccacgcga cctttcacat caccgtcagg aatggcgtac aaacttaagg
     2701 atggatcctt tttcagaccg ttgacgtccg gaggtggggt gatattcagc tggtcgtcag
     2761 tcagttcgat accgagattt ttcgccaccg cctgggccag agtgagatca atatgcgcca
     2821 gctggtcaac aacgcgctca cgaatatacg gacgaacgac tttgcttaac tcaaaactga
     2881 aaccatcgac aatatgacgc tgctcgaatg gcgtctgact tagccagaac agacgcggat
     2941 gggaataata ttcgccaaaa gatggactgc gctcgcgaac tttatttcct tccacgcgtt
     3001 cctgatatga ttcaaaaccg ccgcgtttcg gtcccggcgg tgtttcgcgc ggccagttat
     3061 cgttgatcga gttcggttcg taattcgccg ggttagtgtc gatccccata cgatgcatgc
     3121 catcacgctg gaagttgtgg taagggcagg tcggacggtt aatcggaatc tcatggaaat
     3181 tcggcccacc aagacgactg atttgtgtat cggtatagga gaacaaacgt ccctgcaaca
     3241 gcggatcgtt ggtgaagtcc agtcccggca cgatatgccc aggatggaaa gccgcctgtt
     3301 cgttttcagc aaagaagtta tccgggttgc gattgagcac cattttgccg acacgctgaa
     3361 cgggcaccag ttcttccggg ataagtttgg ttggatcgag aagatcgaag tcgaacttga
     3421 attcatcttc ttcaggaatc aactggaagc ccagttcgta ttccggaaaa tcgcctgctt
     3481 caatggcttc ccacaactcg cggcggtgga agtccgggtc acgtccggtt agtttttgtg
     3541 cttcatccca aacgagtgag gctttacctg ccagtggttt ccagtggaaa cgtacaaacg
     3601 ttgccttccc ttcggcatta atcaggcgga aggtatgaat accaaagcct tccatggtgc
     3661 ggtaactgcg ggggatgccg cgatccgaca tcgcccacat cacgttgtgc agagtttcag
     3721 gttgcagaga aacataatcc cagaaagtat cgtgggcgct ttgcccttgt ggaattgccc
     3781 agtgtggttc tggttttacc gcatgaacaa aatcggggaa tttatgcgca tcctggataa
     3841 agaagattgg cgtgttattg ccaacaaggt caaaaatacc ctcttcggta tagaatttgg
     3901 tggcaaagcc acggatatca cgcacggtat cggcagagcc agcaccaccc tgaacggtag
     3961 agaacctaac aaatactggg gtgattttgt tcggatctga gaggaaatcc gctttggtga
     4021 tatcgcttaa gcttttatat ggctggaaat aaccgtgagc tgctgatcca cgtgcatgaa
     4081 caatacgttc cgggatgcgc tcatggtcaa agtgggtgat tttctcgcgc agaataaaat
     4141 cttccagcag cgttggacca cggctaccgg cacgcagtga gttttgatcg tcggcgatgc
     4201 gcacgccctg attagtggtc agcgcataat tttcactgcc tttgcgtacg tcttccagag
     4261 aattaagttt ttcgttacgc gtatcagggg ctttcaggct ccctggggcg gtaggttgtg
     4321 cacctggcgg tgttggttca gccgctggac gatgagagcc gtcctcaggt gccagtgagt
     4381 ccatccccgg tttcgcttcg ctggaatcgt gtagtggtga ctggtgctga tgtgggttct
     4441 tttcgttatg ttgcgacatt gaactcgtct ccttaattta ttactgaaag ggccgctgtt
     4501 ttggtcaaaa aactctctaa ctatagacca ccagccagat cccacttcgg agactattct
     4561 ggaaacggtg ttatgaaata cacgcaaagc gggacgtaca ggggctaaat cggctaaact
     4621 acagtttttc tgatttttaa tatgcgttta gtgaagccag taatgaagaa accgctccgc
     4681 cagcaaaacc gccagattat tagctatgtc ccacgcacgg aacccgcgcc gccagaacat
     4741 gcgataaaga tggattcgtt tcgtgatgtc tggatgctgc gtggcaaata tgttgcgttt
     4801 gtactgatgg gagagtcatt tctgcgctca ccggcgttta ctgtgcctga atccgcccag
     4861 cgttgggcaa atcagatccg ccaggaaggg gaagtgactg agtaaggatt acgtacctcc
     4921 cggttttgcc gggagttaca tggcctgaaa ttttgtattt cattgctaca ttcattcaaa
     4981 ccacctgtaa caaaaagctg cctt
//
LOCUS       DAZUCE010000298         4993 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZUCE010000298 DAZUCE010000000
VERSION     DAZUCE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.300"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..903
                     /locus_tag="ACHY03_004708"
     CDS             <1..903
                     /locus_tag="ACHY03_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS3907851.1"
                     /translation="QTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRL
                     YIAGIGAGIENTPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYL
                     TAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSAN
                     QIEGDIVKTVGKAFPRDSRAPERWPSGTITVRIYDDQPFDRQIVIPAVAFSGAKHERE
                     HTDIYSSCRLIVKKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNDW
                     YPTASISDLLVVVMKKATAGISIS"
     gene            971..1570
                     /locus_tag="ACHY03_004709"
     CDS             971..1570
                     /locus_tag="ACHY03_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS3907852.1"
                     /translation="MRKVCAAILSTAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSVYAMAGVAYSRVSTFFGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            1635..>4993
                     /locus_tag="ACHY03_004710"
     CDS             1635..>4993
                     /locus_tag="ACHY03_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS3907853.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKASEASKSAAAAESSKSAAATSASAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSATSSATAAGNSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKTGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSRDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTV"
BASE COUNT         1299 a         1230 c         1403 g         1061 t
ORIGIN      
        1 cagaccgtca ataagaaact ggaagaccag agtgcagcga tccagcagat acagaaggtt
       61 caggttgata caaataataa tctgaacagc atgtgggctg tgaagctgca gcagatgcag
      121 gacggacgcc tttatattgc gggtatcggt gccggtattg agaacacccc cgacggcatg
      181 cagagtcagg tactgctggc ggcagacagg attgcgatga ttaatcctgc aaatggcaac
      241 acaaaaccga tgtttgttgg tcagggtgat cagatattca tgaatgaagt gttcctgaaa
      301 tatctgacgg ctcccaccat taccagcggc ggtaatcctc ctgcattttc cctgacaccg
      361 gacggaaagc tgaccgctaa aaatgcagat atcagtggca gtgtgaatgc gaacgccggg
      421 acgctcaaca acgttacgat aaatgaaaac tgtcagatta aagggaaact gtcagccaac
      481 cagattgaag gcgatattgt caaaacagtg ggtaaggctt tcccgcggga ctcccgggca
      541 ccggagcggt ggccatcagg gaccattacc gtcaggattt atgacgatca gccgtttgac
      601 cggcagattg ttattccggc ggtggcattc agcggtgcta aacatgagag agagcatact
      661 gatatttact cctcatgccg cctgatagtg aagaaaaatg gtgctgaaat ttataaccgt
      721 accgcgctgg ataatacgct gatttacagt ggtgttattg atatgccagc tggtcacggc
      781 cacatgacgc tggagttttc ggtgtcagca tggctggtaa atgactggta tcccacagca
      841 agtatcagcg atttgctggt tgtggtgatg aagaaagcca ccgcaggcat cagtatcagc
      901 tgaattttat aacccatata cgggcgccag aaatggcgcc ttttttattg cagaaaagcg
      961 agaggtaatt atgcgtaaag tttgtgcagc cattttgtcc acagccatct gtctggccgt
     1021 atccggtgcg cctgcatggg cgtctgagca gcaggccaca ctgagcgcag ggtatcttca
     1081 tgcccgtacg aacgctcccg gcagcgataa tctgaacggg attaacgtga aataccgtta
     1141 tgagtttacg gacacgctgg ggctgattac gtcattcagt tatgccaacg ctgaagatga
     1201 gcaaaaaacg cattacagcg atacccgctg gcatgaagat tccgtgcgta accgctggtt
     1261 cagcgtaatg gcggggccgt ctgtgcgcgt gaatgaatgg ttcagcgtgt atgcgatggc
     1321 gggtgtggct tacagccgtg tgtcgacttt cttcggggat tatctccgcg taactgacaa
     1381 caaggggaaa acgcacgatg tgctgaccgg aagtgatgac ggtcgccaca gcaacacgtc
     1441 tctggcgtgg ggggctggcg tgcagtttaa cccgaccgaa tccgtggcca ttgatattgc
     1501 ttatgaaggc tccggcagtg gcgactggcg cactgacggt ttcatcgtgg gtgtcggtta
     1561 taaattctga ttagccaggt aacacagtgt tatgacagcc cgccggttca ggcgggcttt
     1621 tttgtggggt gaatatggca gtaaagattt caggtgtact gaaagacggc acaggaaaac
     1681 cggtacagaa ctgcacaatc cagctgaaag caaaacgtaa cagcaccacg gtggtggtga
     1741 acacggtggc ctcagaaaat ccggatgaag ccgggcgtta cagcatggac gttgagtacg
     1801 gtcagtacag cgttattctg ttggtggaag gattcccgcc gtcacatgcc gggaccatca
     1861 ccgtgtatga agactcccga cccggtacgc tgaatgattt tctcggtgcc atgacggagg
     1921 atgatgcccg gccagaggca ctgcgccgtt ttgagctgat ggtggaagag gtggcgcgta
     1981 acgcgtccgc ggtggcacag aacacggcag ccgcgaagaa gtcagccggc gatgccggca
     2041 catcagcccg tgaggcggca acccatgcga ctgatgctgc aggctcagca cgcgcagcca
     2101 gcacgtcagc cggacaggcc gcgtcgtcgg ctcagtcagc gtcttccagc gcaggaacgg
     2161 catcaacaaa ggccagtgaa gcatcgaaaa gtgctgctgc tgcagagtcc tcaaaaagcg
     2221 cggcagctac cagtgccagt gccgcgaaaa cgtcagaaac gaatgccgca gcgtcacaac
     2281 aatcagcagc cacttctgca tccaccgcga ccacgaaagc gtcagaagct gccacctcag
     2341 cccgggatgc ggcggcctca aaagaggcag cgaaatcatc agaaacgaac gcatcctcga
     2401 gcgccagtag cgcaacttcc tcggcaacgg cggcaggaaa ttccgcgaag gcggcaaaaa
     2461 cgtccgagac gaacgctaag tcttctgaga cggcagcggg acagagcgcc tcagctgcgg
     2521 caggctcaaa aacagcggct gcatcatctg ccagcgccgc gtcaacaagt gccgggcagg
     2581 cctcagccag tgccaccgcc gccggaaaat cggcagaaag cgccgcatca tccgcttcaa
     2641 cagccacaac gaaggctggc gaagccactg aacaggccag cgcagcagcg agttctgctt
     2701 ccgcagcgaa gacatccgaa acgaacgcga aagcgtcgga aaccagcgca gaatcctcaa
     2761 aaacggctgc cgcatcgtca gccagttcgg cggcgtcatc ggcatcatcg gcgtctgctt
     2821 caaaagatga ggcgaccaga caggcgtcag cagcgaaggg cagcgccacg acagcatcca
     2881 cgaaggcgac agaggcagct ggcagtgcga cggcggcagc acagagcaaa agtacggcgg
     2941 aatccgcggc aacgcgcgcc gagacagcgg caaaacgggc agaggatatt gcatccgccg
     3001 tggcgcttga ggatgcgagc acgacgaaaa aggggatagt acagctcagc agtgcgacca
     3061 acagtacgtc tgaaacgctg gcggcaacgc caaaggcagt aaaatcagcc tatgacaatg
     3121 cagagaaacg tctgcagaaa gaccagaacg gcgctgatat acccgataag ggacgcttcc
     3181 tgaacaacat taacgcggtc agtaaaacag actttgctga taagcgtggt atgcgttatg
     3241 tgcgggttaa cgctcctgca ggtgcaacat ctggaaaata ttaccctgtt gttgttatgc
     3301 gttctgctgg ctcagtaagc gaactggcat caagggtcat tatcaccacg gcaacgcgaa
     3361 ccgcaggcga tccgatgaat aactgcgagt ttaacggatt tgttatgcct ggtggctgga
     3421 ctgacagggg gcgttatgct tatggaatgt tctggcaata tcaaaacaat gaacgagcca
     3481 tccactcaat aatgatgagt aataagggcg atgatttgcg ctctgtgttc tatgttgatg
     3541 gcgctgcttt ccctgttttt gcgtttatcg aagatggcct gtcaatatcc gcacctggtg
     3601 ctgatctcgt tgttaatgat acgacctata agtttggggc aacaaatcca gcgactgaat
     3661 gtatcgcggc ggacgttatc cttgatttta agactgggcg tggtttttat gagtctcatt
     3721 cgttaatcgt taacgataat ttgtcgtgca aaaaactttt tgccacagac gaaattgtag
     3781 cgcgtggtgg taatcagatt cgaatgatag gtggggagta tggtgcatta tggcgtaatg
     3841 atggcgctaa aacttacctg ctgcttacca atcaaggtga tgtttatggt ggctggaata
     3901 cattaagacc gtttgctatt gataacgcaa ccggcgaact ggttattgga accaaactgt
     3961 ccgcaagtct gaacggtaat gcattaacag caacaaagct gcaaacgcca agactggttt
     4021 ctggtgttga gtttgatggt tccagagata ttactttaac cgccgcgcat gtggctgctt
     4081 ttgccagaag ggcaacggat acatatgccg atgcggatgg tggcgttccc tggaatgccg
     4141 aatcaggtgc ttacaatgtc acccgctctg gcgacagcta tattctggtt aacttctata
     4201 ccggagtcgg aagttgccgg accttgcaga tgaaggcgca ttacagaaat ggtggtctgt
     4261 tctaccgttc ttcaagagac ggttatggtt ttgaggaaga ctgggcagaa gtttatacct
     4321 cgaaaaatct tccaccagaa agctacccag tcggcgcacc aatcccgtgg ccatcagata
     4381 ccgttccgtc tggttatgcc ctgatgcagg ggcagacttt tgacaaatct gcttacccga
     4441 aacttgcagc cgcttatccg tcaggcgtga tccctgatat gcgtggctgg acgattaagg
     4501 gcaaacctgc cagtggtcgg gccgtattgt ctcaggaaca ggacggcatt aaatcgcata
     4561 cccacagcgc cagcgcatcc agtacggatt tggggacgaa aaccacatcg tcgtttgatt
     4621 acggcactaa atccacgaat aacactggtg cgcataccca tagtttaagt ggcagcacga
     4681 atgcagctgg taatcacagc catagagatg gccgtcgatt taaccccagt gtttttaaag
     4741 atacttatca atatggttat acaagctcag gtcaaaatac ctggggtgta caaggctcag
     4801 taggtatgtc tacggggtgg ttagctaata ccagtacaga tggtaatcat agccactcac
     4861 tgtccggcac agcagcatct gcaggtgcac acgcgcatac tgtcggtatt ggtgctcata
     4921 cgcactccgt tgcgattggt tcacatggac acaccatcac cgttaacgct gctggtaacg
     4981 cggaaaacac cgt
//
LOCUS       DAZUCE010000299         4910 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZUCE010000299 DAZUCE010000000
VERSION     DAZUCE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.301"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..782
                     /locus_tag="ACHY03_004711"
     CDS             <1..782
                     /locus_tag="ACHY03_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFS3907854.1"
                     /translation="SSRFMQSLQQVLNKNEFQLLLGCHEYNQLKEAEILMTLLQGNPA
                     ALVIFGSQLADKTHQILEKTNIPTINVVGSPFSPAQITIETAFFEASHKLTEHMLEQG
                     YKNIGFIGAHMDNRLQRQQLNGWHKAMLSYYQNSDLVITMPGAASLQLGRYALNEILL
                     RQPELDAVICSHEEIALGIMFECQRRLLKIPGNIAVACLDGSDSCDQTHPTLTSIRID
                     YKKMGTETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            985..1665
                     /locus_tag="ACHY03_004712"
                     /pseudo
     CDS             985..1665
                     /locus_tag="ACHY03_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803817.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1682..1990
                     /locus_tag="ACHY03_004713"
                     /pseudo
     CDS             1682..1990
                     /locus_tag="ACHY03_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2093..3199)
                     /locus_tag="ACHY03_004714"
                     /pseudo
     CDS             complement(2093..3199)
                     /locus_tag="ACHY03_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3965..>4910
                     /gene="ireA"
                     /locus_tag="ACHY03_004715"
     CDS             3965..>4910
                     /gene="ireA"
                     /locus_tag="ACHY03_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFS3907855.1"
                     /translation="MKNKYIIAPGIAVICSTVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKS"
BASE COUNT         1351 a         1112 c         1140 g         1307 t
ORIGIN      
        1 ccagctcccg ctttatgcag tcgttgcagc aggtgcttaa taaaaatgaa ttccagctac
       61 tgcttgggtg tcatgagtat aatcagctca aagaagctga aatcctgatg acgcttcttc
      121 aaggtaaccc ggcggcgtta gttatttttg gttctcagtt ggctgataaa actcaccaga
      181 tactcgagaa aacaaatata cctaccatta acgttgtcgg ttcccctttc agtccggctc
      241 aaattaccat cgaaacagca ttttttgaag catcacataa actgaccgaa catatgctgg
      301 aacagggtta taagaatatc ggatttatcg gtgcgcatat ggacaaccgc ctgcaacgtc
      361 agcaactgaa cggctggcat aaagcaatgc tgagctatta ccagaactct gatcttgtta
      421 tcaccatgcc gggtgccgca agtttgcaat tggggcgtta cgcattaaac gagatattac
      481 tgcgacaacc agagctggat gctgtgattt gcagccacga agagattgcg ctgggtatca
      541 tgtttgagtg ccagcggcgt ttgttaaaaa tccctggaaa tatcgctgta gcttgtcttg
      601 acggttcaga cagttgcgat caaacacacc caaccttgac atccatccgt attgactaca
      661 aaaaaatggg gacagagacg ggtaagctgc tgattggact attgaataat aatcatgatg
      721 aatcagaaga gtcgcgtatc gtgcaattca actaccagat agaattacgt cagagtacat
      781 gagctcaaaa ggggattatt gcaaagttag ctattgtggt agggatagcc gtttccggct
      841 attccccgcg cagagccgga tgtgtgctgc taacgcatcc tgtcaacgac ggatgaaaag
      901 ttatccactt atatctccac caacggccca atattgtacc gttcgtgaca catccatttc
      961 tacttcaggt atttggcgta gtagcgggtg ctaaaaattt gccgcaaaac cgcaatatca
     1021 gcccgcagag gcgctctcca gattatccat tcctatatta tctctgcacc ggatgaatta
     1081 ctcgaacaac tcagaaatat gacacatatg caacttatca ggactctggg atcctggagg
     1141 cctgatgcca gtgaatacag taatgttacc aacgtttatc gcattacatt gaaatccctt
     1201 gttcgacgct atttcgagtt tcatgatgaa atcaccaact tggatgtcat gattgcggca
     1261 attgttgatg aactggcacc tgaactgatt aaacgtaatg ctgttggata cgaaagcgct
     1321 ttgcagttgt tgatctctgc cggagacaat ccctaacggt taagaaccga atcaggtttt
     1381 gcggcgttgt gcggtgtcag ccagtttccc ctatcttctg gaaaaacgaa ccattaccga
     1441 cttaatcggg gtggggagcg tgttgcaaat agtgcacttt acatcatcac tataggatgt
     1501 ttacaaactg acgataaaac aaaggaatat gtctccaggc gagtagccga aggacataca
     1561 aaaatggaag caatacgctg tctgaagcgc tatatctcac gcgaagttta tacattacta
     1621 cgaaatcaaa acaggcatgt taactgcatc ccgataacgt cttgactctt agaagggcgc
     1681 ccaaggcagt cattattcga gcaggcagtt ccggcagtta ttgtgtcgat accagatcag
     1741 gcagagtatg agccggcgcg gaaaatgctg ggataacagc tcaatggaac gcttcttcag
     1801 gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg aggcagctca
     1861 ctccataacg gacgatatcg ttggatatta cagcgcacta agaccgcacg aatataacag
     1921 tgggttatcc ccaaacgaat cggaaaatcg atactggaaa aactctaact cggtggccag
     1981 tttttgttga ccacttcagc catcgccaga cgctaacatg agttactctt gttctgagct
     2041 acgtcacctt cagggaactg gttatcctca ttctttattt gtgacattgt ggttattgtg
     2101 cgacaaacgt ggctatcccg ccccatatcc cagataaaac cacctgctca cgtgcgacag
     2161 cgacaattgt aatattgaca ttctttcctt tggcctgaag ttttcgagac ctcttgcaaa
     2221 gcctgagttg agcatcccat gctctgtcaa tgacggggcg gggtaaattt tcctgatttt
     2281 ttgtattgcc ggactgatgc gtgtcggatg ccggtaactc cacgctgcct caaccaatag
     2341 ctttctggca tagctgtttc cggctttggt aatgctgccc tgatgtctgc ttccacccga
     2401 tgaatattct gatggcgtga taccaaacca tgacatcagt tgtcgtggat gttcaaaacg
     2461 ggtcagatca cccagttcag aaattaatcc aaccgcagta ataaactgaa tccctcgcat
     2521 ggcttgctgc gcctctacta ccggatacag cctccattcg gtaacggcct ccttcgggcg
     2581 gattccagcc gttcacattg tgcctgccga tcttctattg tacaacggtg ttcgtcaaaa
     2641 gctaattgtc gccagggact ttcgaatgag tatttgctga gccagtgtcg gtgtgcaggg
     2701 ccccaatctg ctcgcccgac ataatggacc ccatgaacca gaagaaacga tttcagacgt
     2761 tgccttgcat gccgtaaatc atcgcgggca gatgcccatg cctgggccaa atcccggaat
     2821 gcctcatctt caacgccgga tacgtagaca gcagaaagat ctcctgcacg cagtgaacgc
     2881 acaagtctga tggcatcacg gtgtcggttt taccccgctc ccccggtttt ttcgggataa
     2941 gtgagggggc gcagaccatg cagtcaaaac cggatttcac cagccggcga taaagtccat
     3001 aaccacaggg tcccgcttta taaacgatac tgacctgcga tgactttgac ctgatacgtt
     3061 gataaagatt atgaatgtca gtaggtgatg tgctaatttt acccatcagt tcaacaggtt
     3121 ctgaattgat agcataggag acagtaatcg attctttgtg aacatctaat tcgacgtaca
     3181 acatgttatt gttgttcatg ctggcctccg tggtggaaat tccagggggg tagcaacagc
     3241 tcacccatgt ggctctgaca acgatgttaa cccgcattaa atatcccacg ttggacagca
     3301 tctgtctcac ggaaagtcat actgccttac gtgcatattt caccttacgc cttcactgtc
     3361 tgtcatcgga agtactgatt tatattgggg ctttgcactt actgtcattc acgggaaaag
     3421 atggtggtga acagtacgat actgtcttgg ctgttgccga gggaagattc aggagtggta
     3481 agggggttag tggtgtaaga tgagtctgga gctctgactg gtcagtgacg cgaaactcaa
     3541 tctgccagga taaactcccc cttgcttttg cgcagaagca gtgggccttg gttcgcttat
     3601 gtcctgcatc tacaagcgaa tgactgaccg gagactccca tatctggtca gtcgcccatt
     3661 tttattttcc accacatcgg acaagcatac cgtaataaac agcaccagtc tcctgttcac
     3721 tcttgcttga tttactggaa tgctgtttat ggagatatcc ggttgaaaga ttttacctct
     3781 tcgacaggac tataaattat acagtaagga gtccctcgta gaaaggggct gattctcatc
     3841 aagtcggaca ggttcaaagt gttacctgaa tgagctaaat gttatttacc ttcctttctg
     3901 ttcattgtaa atttttcttg ctaacgcaaa tcattatcat tacgatttta gaaaagggaa
     3961 atttatgaag aacaaatata tcattgctcc gggcattgcc gtgatatgtt ctacagtcat
     4021 atcatcaggt tatgccagtt ctgataaaaa agaagatacg cttgttgtta ctgcctccgg
     4081 cttcactcag caactcagaa atgcaccggc cagtgtttca gtcattactt cagagcaact
     4141 gcaaaaaaaa ccggtttcag acctggtcga tgcggtaaaa gatgttgaag ggattagtat
     4201 caccggtggg aatgaaaaac cggatatcag tatacgtggg ttaagtggcg attatacatt
     4261 gattctggtc gatggacgac gtcagagtgg tcgggaatcc agaccaaacg gcagcggcgg
     4321 ttttgaagcc ggatttatcc ctcctgtgga agcaattgag cgtattgaag ttatacgtgg
     4381 ccctatgtct tccctgtatg gttccgatgc tatcggagga gttattaata tcataaccaa
     4441 acctgttaat aaccagacat gggatggcgt acttgggctt gggggaatta ttcaggaaca
     4501 tgggaaattt ggtaactcaa ccacgaatga cttctacctg tcaggcccat tggttaagga
     4561 taaacttggt cttcagttat atggaggaat gaactatcgc aaggaagata gtatctctca
     4621 gggaacacca gcaaaagata ataagaatat aacggcaaca ctccagttta ctccgactga
     4681 aagccagaag tttgtttttg aatacggaaa aaataaccag gaacatacat taacacctgg
     4741 tgattctctt gatgcctgga ctatgcgggg aaatcttaaa caaccaaaca gtaaaagaga
     4801 aacacataac tcacgtagtc actgggtagc agcatggaat gcccagggcg aaatactgca
     4861 tcctgaaatt gctgtttatc aggagaaagt tattcgtgag gttaaatctg
//
LOCUS       DAZUCE010000300         4908 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZUCE010000300 DAZUCE010000000
VERSION     DAZUCE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.302"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..872)
                     /locus_tag="ACHY03_004716"
     CDS             complement(<1..872)
                     /locus_tag="ACHY03_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFS3907856.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHVDGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPV"
     gene            complement(884..2098)
                     /gene="rspA"
                     /locus_tag="ACHY03_004717"
     CDS             complement(884..2098)
                     /gene="rspA"
                     /locus_tag="ACHY03_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFS3907857.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2304..2630)
                     /locus_tag="ACHY03_004718"
     CDS             complement(2304..2630)
                     /locus_tag="ACHY03_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFS3907858.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRA"
     gene            2765..3092
                     /locus_tag="ACHY03_004719"
                     /pseudo
     CDS             2765..3092
                     /locus_tag="ACHY03_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
     gene            3127..3687
                     /gene="speG"
                     /locus_tag="ACHY03_004720"
     CDS             3127..3687
                     /gene="speG"
                     /locus_tag="ACHY03_004720"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFS3907859.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3690..4400)
                     /locus_tag="ACHY03_004721"
     CDS             complement(3690..4400)
                     /locus_tag="ACHY03_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFS3907860.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4508..4813
                     /locus_tag="ACHY03_004722"
     CDS             4508..4813
                     /locus_tag="ACHY03_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFS3907861.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSNQDGVPAEPQ"
BASE COUNT         1340 a         1252 c         1075 g         1241 t
ORIGIN      
        1 accggaaatt tatttgcatt taagcgtgaa gagaaaatag agagttcttt tccggtaatt
       61 ccttgctgaa tcacttcaga aggttcactg gagaagccca tcaatacaat acgtgccgct
      121 ggagaagcca gcgttacggc ctctttcagg atagaaggat gacaagccgc atcaataatt
      181 aatgtcggct tgatgccttt ttcagtgaaa atctcgccaa gcggtgtctg gctgttatta
      241 atcgcccagt cagccccgct ctctttcgct ttttccagtc gttcatcaat gcgatcggca
      301 acaataacat ttttaacgtt atagacgcct tttaatacct gaacgatcgt caggccgatt
      361 ggaccggcac cataaaccag aacggtatca ttttctgtcg gttgaccatg accggttacg
      421 ttagccgcaa tggtaaaagg ttcgatcatc accgcatatt gatcggccac tgcttcagga
      481 attttccacg cattttttgc cggaaccaca gcatattcac tgaaaccacc gtcaacgtgc
      541 acacctaata cagcgagtgt cgtacaaacg ttcggcttac ctatagagca cggatagcaa
      601 tgaccacagc tgaccaccgg atcgacagca acacgttcac cgactctggc gctttccaca
      661 ccttcaccca ccgcatcaat gacgccaaag aattcatgac caatgacgcg cggatatttc
      721 gcaaaaggat tatgcccacg ataaatatgg ctatctgaac cacaaattcc ggcaagtttc
      781 acttttactc gtacttcacc cgctgacggg gtgggtattt cacgttcgac aatcgccagt
      841 tgattcggtt tttcaattaa tatgcttttc attatcttac tccttaccag ttccacagcg
      901 tgccatcttc cagacgtgcg actggtagat aagcaggttc ataggggtat ttcgccgcca
      961 gcttttcatc gaattcgatg ccaagacccg gtttgtctcc cggatgcata tagccgttat
     1021 cgaaagtcca gttgtgcggg aagacttcga gcatttgttc ggaataaccc atgtattcct
     1081 ggacaccgaa attggggacc cacagatcaa agtgcagcgc cgcagccatg cagactggtg
     1141 acaaatcgga aggaccgtgt gagccagtac gtacctgata caacgaagca aaatcggcaa
     1201 tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata taatcgataa
     1261 gttgctcttc aatcagttgc ttgcagtccc agatgctgtt gaagacttca cccaccgcga
     1321 tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc gcaggcgtcg
     1381 ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc gccgcttcaa
     1441 taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca aacttgttac
     1501 gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac cacagctgct
     1561 cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct ttcgacatgc
     1621 cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg aatcccagct
     1681 cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg gtatggcaat
     1741 aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc atgttggcag
     1801 ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac atcgtaaccg
     1861 gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg atacggtgcg
     1921 catcgcgacc aataagctgc ggacaaaggt gatcctgcaa ataagaggcc acggaaagct
     1981 cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg gtgattttta
     2041 atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctttacg atcttcatgt
     2101 tcgattcctt gcatcgcttg tcgtgatgca tgaaatctac gcaactgagc tactaccata
     2161 caagtatgaa gatcaaaaat agccggagtg atcacaaaaa aaggcgtata tttgcgctgt
     2221 gaatggttga caaaagatga aatagaatac cttttgtcag ctgacacatc ctcgtatctt
     2281 attgataaaa tagatttatg tccttacgcg cgcccccagc ccgcaacaat gatcaacatg
     2341 ccgcaaagcg caatcaacgc acccgtccag tcataaagag tcagtttcac gccatccaca
     2401 acgcgcagcc acatcaacgc cgtgcagaca taaacgccac cataagccgc gtaaacacgc
     2461 ccactcgccg caggatgcaa cgttaataac cagacaaaca gcgccagtga aatccccgcc
     2521 ggaagcaaca gccagatact ggcgtttcgt tttaaccaca accagggcag aaagcatcca
     2581 ataatttcac acagcgcagt agcaaaaaat agtaacgttg ttttaatcat ctttgtcact
     2641 tattgacatc atgtatagtt atagggcgac ataatatcat caatataaac accctcctgg
     2701 tacgttttgc gttcgcagtg gatggtgtag aatcaccttt aatcattcat acagggaatg
     2761 aattatgaaa atcactctca gcaaacgaat cggcctgctc gcttttctgc tgccttgtgc
     2821 actggcattg agcacaactg ttcatgccga aactaacaaa ctggtgattt gcacaaagcc
     2881 gccagcacgc cgctatggaa aaagagcaat ggaatgacac gcgcaatctg cgccagaaag
     2941 tgaataaacg cactgaaaaa gagtgggata aagccgacgc cgcttttgat aaccgcgata
     3001 aatgcgagca aagcgccaac atcaatgcct actgggagcc aaatactttg cgctgcctgg
     3061 accgtcgaac tggccgcgtt attaccccct aacctgttat tgatttaagg aatgtaagga
     3121 cacgttatgc caagcgccca cagtgttaag ctacgcccgc tggagcgtga agatttacgc
     3181 tatgtacatc aactcgacaa taacgccagt gtgatgcgtt actggtttga ggaaccctac
     3241 gaagcctttg ttgaactctc tgatctgtat gataagcata ttcacgatca gagcgaacgg
     3301 cgctttgtgg tggaatgtga cggcgaaaaa gccggtctgg tggagctggt ggaaattaac
     3361 catgttcatc gccgcgcaga atttcagata attatctccc cggagtatca ggggaaaggt
     3421 ctggcaaccc gtgccgcaaa attagcaatg gactatggct ttaccgttct caatctctat
     3481 aagctgtatc tgatcgttga taaagagaat gaaaaggcga ttcatattta ccgcaagctt
     3541 ggctttacgg ttgaaggtga attgatgcac gagttcttta ttaatggtca atatcgtaat
     3601 gccattcgca tgtgcatatt ccagcatcag tatctggcag agcacaaaac accgggtcag
     3661 actctcctga agccgaccgc acaatagcat taataataat cgatcgtatt tttgatggtg
     3721 taaacccgtt cgacggcggg ttttactcct tcatcaacaa tgattagctg acagtccacc
     3781 ggattagcgt gactgtcata ttcacagctc tgttttacat taccaacccg ttgattgttc
     3841 agtaaagtaa ccgctgtgta atctaatttt ttgatcggat ccgttgatgg cgtggcgctg
     3901 acagataatg ttttgtcgtt acttttcgtg gttttaccca gcggataccc ctgatcatca
     3961 tagcgatatt ccatctgcat ctgtttgctg ctggctttaa tcacgaagcc attatcatcg
     4021 gtttcccaac tcaccccggc ggaaggtaat tccgctagct ggcattttcc ctgtaaacgt
     4081 actctcttct ccagcgtctc ggcatcacgg taataattgg cgtccagtac cagagcgacc
     4141 acggtattat tttccagatc cagtaattcg agtgaatcaa aacagccttc ttccgacaaa
     4201 gtcccagaaa cacgtttcgt cacttcacct tgctcatcca ttaatgtctg agtgaaatct
     4261 tttaccggac cacgcagcgg atcaaaatca aattcattag agaaactggc catctcaggg
     4321 gtaaatgaaa gtgttacctc tgtgcggtca catcctgtga ggaatatcgc gagtaagcat
     4381 ggtagtaatt tgtatttcac aacagtcacc agagagtaga gatgattctc aatcatagta
     4441 gcaaatacag tactttacac gttaaatgct atgcttaaag aagttatctt cgcgtaagga
     4501 gcttatgatg aaactctcaa cctgctgtgc cgcgcttctg ctcgccctcg cctcacccgc
     4561 ggtacttgcc gcgccgggat cctgtgagcg catacagagc gatatatcac aacgcattat
     4621 caataatggc gtacccgaaa gcagcttcac gttaagtatt gtacccaatg accaggttga
     4681 tcagcctgat tctcaggtcg tcgggcattg cgctaatgat acgcataaaa ttctctacac
     4741 ccgcaccacc agcggtaacg tctctgctcc cgcgcagtct aaccaggatg gcgtgcctgc
     4801 cgaaccgcag taatacatta tcgactgaac gccggatatg acacatccgg cgatttgaac
     4861 atacaacata atcccaccat attactcata cccttctatt gatgtgga
//
LOCUS       DAZUCE010000301         4803 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZUCE010000301 DAZUCE010000000
VERSION     DAZUCE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.303"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..804
                     /locus_tag="ACHY03_004723"
     CDS             <1..804
                     /locus_tag="ACHY03_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFS3907862.1"
                     /translation="FPGLSILVFNVLMTNKKYMPLLELINKEKPDILVLLETNLWWEQ
                     KLSPLDEVFPYSLKCPKENLYGMHVYSRMILSDAEIQYLVDKEIPSMHMIVHMPEGQR
                     IRLHCLHPVPPSPTESDESTDRDSELLMVGKSVADSELPVILAGDLNDVAWSATTRLF
                     MKISGLLDPRRGRGFFSTFHSHYFFLRWPLDHVFHSQHFLLKSLRRLPDMGSDHFPVM
                     AELVYAPAHGMQQESLEKEKSYEVLAQKKMDMTDSTAEDVHTPGGQKKS"
     gene            1949..2176
                     /locus_tag="ACHY03_004724"
     CDS             1949..2176
                     /locus_tag="ACHY03_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFS3907863.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
     gene            2526..3101
                     /gene="dolP"
                     /locus_tag="ACHY03_004725"
     CDS             2526..3101
                     /gene="dolP"
                     /locus_tag="ACHY03_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFS3907864.1"
                     /translation="MRLLYSAVVLTSALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVKEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADIASRVSGVKRVTTAFSCIR"
     gene            complement(3531..>4803)
                     /locus_tag="ACHY03_004726"
     CDS             complement(3531..>4803)
                     /locus_tag="ACHY03_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3907865.1"
                     /translation="KISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAAKAN
                     FAVAVGRYSQANAEKSISLGASARAIENNSTALGANATTLKPFSVALGAGSVADRGAG
                     IEAYMPETKRWNLGDVVKSRMSDKQLNRLMEIDKLISAYNSQGTLSAEETKQLEEVKK
                     ERADMFATYIATHDAVSVGVGATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQTV
                     TEKADAALDKRITNNTSAITKNLQVVNSNKEEISNINKTTKILDERVTANTQTAEVHK
                     LLISENSKQISTNTLGIRENTQRIRDEGQARIRGDRETLHAATGYTDMRVNGLERNTN
                     KRFAQLKEDIDKNRKRADAGIAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAGFH
                     FNTSENSAVKVNAAWDTENGAGVSAGMALGW"
BASE COUNT         1268 a         1048 c         1092 g         1395 t
ORIGIN      
        1 tttccgggtt taagtatact ggtattcaat gtccttatga ccaataaaaa atatatgcct
       61 ttgcttgaac ttataaataa agaaaaacct gatatacttg ttcttcttga aacaaactta
      121 tggtgggaac agaaactatc acccctggat gaagtgtttc catactccct gaaatgcccg
      181 aaagaaaatt tatatggcat gcatgtatat tcaaggatga ttttaagtga tgcagaaata
      241 cagtatttag tggacaagga aattccctcc atgcatatga tagttcatat gccggaaggt
      301 cagagaataa gattacattg tcttcatcct gttccaccga gcccgactga aagtgatgag
      361 tcaactgatc gtgactcaga acttttaatg gtcgggaaaa gtgttgcaga ttctgaatta
      421 cctgtaatat tagcggggga tctgaatgat gttgcgtggt ctgcgaccac tcgtttattc
      481 atgaaaataa gtggactgct ggatccacgc cggggacgtg gtttttttag cacgtttcac
      541 agccattatt ttttcctgcg atggccgctt gaccatgtat ttcacagcca gcattttctg
      601 cttaaaagtt tacgccgttt accggatatg ggttcagatc attttccagt gatggctgag
      661 cttgtatatg ctccggctca cgggatgcag caggagagtc tggaaaaaga aaaatcatat
      721 gaagtactgg cacagaaaaa aatggatatg acagactcaa cggcggaaga tgttcatact
      781 cctggcgggc agaaaaaatc ctgacgtaaa tatattaatt acgggacata ttcagatacc
      841 tgacatatga ttaattcttt tttacgtctc tccagaaact ggggggataa gggaaagtcc
      901 ccctgagggc tccccggaaa aagagacagg gattatgagc tgactagttg tactgataat
      961 ttcaggggaa cagccacgat gcatgcctgt ccattattca gggtactcct tacagacata
     1021 cttccccttg tatctttacc gtcaaaaatc aatttccgta tggtgtaata cagaaagtta
     1081 ctgaagatga taattacatt gatttttagg attatcgtta taatgatttt atataaatgt
     1141 gaaaagcacc tcatttcata aataagcatg ttgtccggtt tctgatgaat ttgctaaaaa
     1201 aggcaggcat ggtgaatccc cctgtgcggt ggggtgatac cattaatttc ctatgttgct
     1261 gactggtatc acgaacggtt tgttcaccgg gaggcacccg gcaccatgcg catgatgata
     1321 cagatacgca gcgtcagccc ctctacggag gggcgttttg ttttgtatat gccaacgcca
     1381 cgatttttga ccccattacc gtcttatatt acaggcgctg acatatataa cgcacagtcg
     1441 tgagactgca gtgctttttg tctgcggatt ccgatgtatt cgatcagttg ttcgcccgac
     1501 gttcgattgg caggatgcga gtcagcaatc gggcatgatg tgatttcgta tttttccact
     1561 gcagctgcag ctgcagcttt ttatcggtaa atgttaaata cttcacagtt cagtcagtgg
     1621 attattgtcg gtcttacggg atatttgtta aaaaaataat gcatggtgaa tccccctgag
     1681 cggcggggca tatcagtatc gtaggtgttt ggtcacatcc cttatctgtg cgggttcagg
     1741 tgctgatact gaactcaccg ggaggcaccc ggcaccatgc tacataacac tccattacgc
     1801 tacactcgcc cctcttcgga ggggttttct ctgcagggta tgaatatgag acgctgcatt
     1861 attcactgcg aatactgatg gtgagaatct ttaataatat taatgtttat atttattgtt
     1921 acagtgttgt tgaaaggaga taaaaacaat gttcccggag tacagagaac tgatttcagc
     1981 attgaaagga aatcataaac gctttgattc tttatttgaa aagcataata aacttgatca
     2041 cgaaataaaa agaattgaag atgagtttgg ttcagcgtat tcgacgaaag tggtcaggct
     2101 gaagcttgaa aaacttgaga tcaaaagaga aatccagaaa atccttgaag atgagtcaga
     2161 aaagggcgaa cggtaatcat aaacggtata ttcaaagccg tgttttctct gttttatgaa
     2221 aaaggcaccg ggggctccac ggtgcctttt agcctgtgta taacgaaaaa ggagatcctg
     2281 ttcagcaaac caggtagtta aaaaaacaga atctttacgg cgatatcaaa gttttcgtgc
     2341 aaaaaacatg atcaatatta aatttcagta catccatatc cggcataaac atttccgtct
     2401 ggcagataca gatatgccac gtaacgtatc cgctgtttcc ggctggggcg gaggagaata
     2461 acagtccgta gcttgttttg tttcactgaa ggtaaacttt ttgtacgata aattccgggg
     2521 gagatatgcg tttattatat tctgctgttg tccttacttc agccttattg ttacaggggt
     2581 gcgtggctgc cgctgtggtg ggtacagctg ctgtcgggac aaaggctgca accgacccac
     2641 gcagtgtcgg aacgcaggtg gatgatgaag tgctggagct gagggtcagc agtgcacttt
     2701 caaaagatga acaaatcaga aaagaagcac gcatcagtga gattgcatat cagggacgcg
     2761 tgctgctgat cggtcagtct ccggaccgtg aattatctgc ccgtgcccgt cagatagcac
     2821 tcggtgtcga tggtgttaag gaggtctata atgaaatccg gcaggggact cccgtcagtc
     2881 cggtcataat ggctggtgat gcctggctca cgacaaaaat tcgttctcag ttactcacaa
     2941 gtgcccgtgt caaatcatcc agtgtcaagg tggtgacaga aaacagcgaa gtctttttac
     3001 tgggcctggt tacagagcag gaagggcgag cggcagcaga tattgccagt agggtgagtg
     3061 gcgtgaagcg ggtcacgacg gcattctcct gtatacggtg aaggccgtat ctgagttgcc
     3121 tttagtgcaa atttacgtta aaaatcagaa gagcacgttt ccgggccgtt catgccacca
     3181 cgtgaacatt cgtcgcggaa taccggagac gtgctcatta taataaccaa tatacataat
     3241 tcctctttga cacccccctg aattcggggg atttttttgc ccgggtatgt tcactcacag
     3301 ataaaatccc ggtgcgctcc ggatgtcgct gctggtaaat aatctgagct tttatccata
     3361 caggtcaaag agcgtggcgt tgacacgtgt acgtctggct ccggtgcagg agccagattg
     3421 tccgtattaa ttacatcctg agccgatgcg ataaactgaa tcgcttcctg aatggtggtg
     3481 cagtgtcccc tggataacaa tatctgcgaa cggcttctgg cccggaagca ttaccatccc
     3541 agagccatcc cggctgaaac gccagctcca ttttcagtgt cccaggctgc attcacttta
     3601 acagcactgt tttcacttgt attaaaatgg aagccggcag caacagcacc ttcactccgg
     3661 tatccggcca tggccatacc aaatgagacc tttttcccgg gtacggacgg tatccccgtc
     3721 attgccatcg cacctgcaat acctgcatct gcacgttttc tgtttttgtc tatatcctct
     3781 ttaagctgcg caaaacgttt attcgtatta cgctccagcc cgtttacccg catgtcggta
     3841 taaccggtcg ctgcatgcag tgtttccctg tctccccgga ttcgagcctg tccctcatcc
     3901 cggatccgtt gtgtattctc tctgatacca agagtgttgg tactgatttg tttgctgttc
     3961 tccgaaataa gcagcttatg tacttctgct gtctgtgtat tggctgtcac tctttcatcc
     4021 agaatttttg tcgttttatt aatgttactg atttcctctt tattactgtt aaccacctgc
     4081 agatttttag ttatcgcact ggtgttgttt gttatgcgtt tatcaagcgc tgcatctgcc
     4141 ttttctgtca cagtctgcaa atccctgagc tgtgcaacgt taacagcatc cgtatcgaca
     4201 gaacctgctg caacactagt tatctgtctt gtaacagcat gtcgtcctgt tgtcgttgca
     4261 ccaacaccta cagatacagc atcgtgcgtt gctatatatg tcgcaaacat atcagcccgc
     4321 tcttttttca cttcctccag ttgtttagtc tcttcagcag acagcgtccc ttgtgaatta
     4381 taagcggata ttagcttatc gatttccata agcctgttaa gctgcttgtc agacatcctg
     4441 gattttacta catcccctag attccagcgc tttgtctctg gcatatatgc ttcaatacca
     4501 gcgccacgat cagcaacact tcctgcgccc agagcgacag agaatggttt caatgttgtt
     4561 gcgtttgctc ctaacgcggt agagttattt tcaatagctc ttgctgatgc acctaaagaa
     4621 atactttttt ctgcatttgc ctgtgaatat cgaccaacag caacagcgaa attagctttt
     4681 gccgcactgt tatcaccaag ttccacgatc attttatcag gaataccatt tgcagataag
     4741 acaggtaaag aagctgagca gtagagaaat aatgatttgc atatataact gacactgatt
     4801 tta
//
LOCUS       DAZUCE010000302         4770 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZUCE010000302 DAZUCE010000000
VERSION     DAZUCE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.304"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            6..81
                     /locus_tag="ACHY03_004727"
     tRNA            6..81
                     /locus_tag="ACHY03_004727"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHY03_004728"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHY03_004728"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHY03_004729"
     CDS             complement(299..1057)
                     /locus_tag="ACHY03_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFS3907866.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHY03_004730"
     CDS             complement(1065..2168)
                     /locus_tag="ACHY03_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS3907867.1"
                     /translation="MTKVLLSHPPRPASHNSNRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHY03_004731"
     CDS             complement(2178..3359)
                     /locus_tag="ACHY03_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFS3907868.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3427..4452)
                     /locus_tag="ACHY03_004732"
     CDS             complement(3427..4452)
                     /locus_tag="ACHY03_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFS3907869.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1306 a         1235 c         1150 g         1079 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggactccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttatgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga cttaggatgc gcaaaaaact
      361 catccggtgc agcttgctcc actatttcgc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gatgtgggct
      541 catcaaacaa cataattttc ggcttcatac acagcgagcg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctaccac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaacctgaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaactga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagagc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg acacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagcc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccagttag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggtttga gttatggctc gccgggcgcg ggggatgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctgatt cagcacggtg ccggcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggt
     2401 ggaataatca cccgcaatgc ttggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa ggagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcaaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccaacttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca tttagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagccggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgggattgat ccgaagccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac tggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatcagt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 ataagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aatatcaaca tcaatacctg aaaatttacc gtcagcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgattagcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccagc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aagtgcgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc
//
LOCUS       DAZUCE010000303         4724 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZUCE010000303 DAZUCE010000000
VERSION     DAZUCE010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.305"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..851
                     /locus_tag="ACHY03_004733"
     CDS             <1..851
                     /locus_tag="ACHY03_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFS3907870.1"
                     /translation="IFEAVYVHKGIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRK
                     GQGVTPTAFAMHLHEYISQGLESILGALDIEGSYDKQRTITIATTPSVGALVLPVIYR
                     AIKTHYPQLLLRNPPISDAENQLSQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRE
                     GNPLLSLEDDRETIDNAAHVLLLPEGQNFSGLRQRVQEMFPDRQISFTSYNILTIAAL
                     VANSDMLAIIPSRFYNLFSRCWPLEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVID
                     VIRNAF"
     gene            1060..2025
                     /gene="lipA"
                     /locus_tag="ACHY03_004734"
     CDS             1060..2025
                     /gene="lipA"
                     /locus_tag="ACHY03_004734"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFS3907871.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNLATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2126..2329)
                     /gene="tatE"
                     /locus_tag="ACHY03_004735"
     CDS             complement(2126..2329)
                     /gene="tatE"
                     /locus_tag="ACHY03_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFS3907872.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2458..3246)
                     /locus_tag="ACHY03_004736"
     CDS             complement(2458..3246)
                     /locus_tag="ACHY03_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFS3907873.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMICYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3339..3722
                     /gene="crcB"
                     /locus_tag="ACHY03_004737"
     CDS             3339..3722
                     /gene="crcB"
                     /locus_tag="ACHY03_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFS3907874.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3776..3985)
                     /gene="cspE"
                     /locus_tag="ACHY03_004738"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3776..3985)
                     /gene="cspE"
                     /locus_tag="ACHY03_004738"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFS3907875.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4160..4720)
                     /gene="pagP"
                     /locus_tag="ACHY03_004739"
     CDS             complement(4160..4720)
                     /gene="pagP"
                     /locus_tag="ACHY03_004739"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFS3907876.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKAFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1235 a         1209 c         1070 g         1210 t
ORIGIN      
        1 ctatttttga agctgtatat gtacataaag ggatcgttaa tgcagcgaaa gtgcttaatc
       61 tgaccccctc ggcaatcagt cagtctatac agaaactgcg cgttatattc cctgacccat
      121 tgtttattcg caaaggccag ggtgtcactc ctaccgcatt tgcgatgcat ctacatgagt
      181 atatcagtca gggccttgag tccattcttg gcgcgctgga tatcgaagga agctatgata
      241 agcaacgaac gataactatc gcgaccaccc cctcggtcgg agccctggtc cttcctgtca
      301 tctatcgggc gattaaaact cactacccgc agcttttact gcgcaacccg cccatcagcg
      361 acgcggaaaa ccaactcagt cagtttcaaa ccgatctcat catcgataac atgttttgca
      421 ccaatcgtac ggtgcaacat catgttctgt tcaccgacaa tatggtgtta atttgccgcg
      481 agggaaatcc actactctct ttagaagatg acagagagac tatcgacaac gctgcgcatg
      541 tactcctgtt accagaaggg caaaatttca gcggtctgcg gcagagagtt caagagatgt
      601 ttccggaccg gcaaatcagc ttcaccagct acaatatttt gacaatcgct gcactggttg
      661 ccaacagtga catgttagcg attattccca gccgttttta taacctgttt agccgttgct
      721 ggccgctgga aaaactgcct tttccgtcct taaatgagga gcaaatagat ttctctatcc
      781 actacaacaa attcagcctg cgtgatccca tcctgcacgg agtaatagat gttatccgta
      841 atgcattttg aaaaagtatt gttacagcca gatcacagcg cagaacgata aaatggcaca
      901 atccaacaac aattttacat tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg
      961 atatactgcc agtcgttaat tcaaaaatag ttgataatta caacaatcta ttgaattgaa
     1021 acgctttcct tcgtaattcg caactggaac acgcacgcta tgagtaaacc cattgtgatg
     1081 gaacgcggtg ttaaataccg cgatgccgat aagatggccc ttatcccggt taaaaacctg
     1141 gcaacagagc gcgaagccct gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg
     1201 gactctacac gtatccaggg catcaaagcc gcaatgcgca aaaatggcct gcattctgtc
     1261 tgcgaggaag cctcctgccc taacctggcg gaatgcttca accacggcac agcaacgttt
     1321 atgatcctcg gcgctatttg tacccgccgt tgtccgttct gtgacgttgc ccacggtcgg
     1381 ccggtagctc ctgatgccaa tgagccagtg aaactggcgc agaccattgc cgatatggcg
     1441 ctgcgttatg tggttatcac ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccag
     1501 cactttgcgg attgcattac tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact
     1561 ctggtgccgg atttccgcgg tcgtatggat cgtgctctgg atattctgac tgcaacgcca
     1621 ccagatgtgt tcaaccataa cctggaaaac gtaccgcgta tttaccgtca ggtacggcct
     1681 ggtgcagatt acaactggtc gctgaagctg ctggaacgct ttaaagaagc acatccggaa
     1741 atcccgacca agtctggtct gatggtggga ctgggtgaaa ccaatgaaga aattattgag
     1801 gtaatgcgcg acctgcgccg tcatggtgtg acgatgttaa cgctggggca atatttgcag
     1861 ccaagccgcc atcacctgcc ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg
     1921 aaagccgaag cgctggcgat gggctttacc catgctgcat gcggtccgtt tgtccgctct
     1981 tcttaccacg ccgatttgca ggcgaaaggg atggaagtta agtaatcctg taataattac
     2041 ttacatctgt ctgataaaaa acccgctttt tgaagcgggt tttttgtatc gaacaagata
     2101 ttgagggagc gtcctgctcg ccacgtcact ctttatgaga gagcttttca gcctgaagat
     2161 caacgtctgc gccttttttc gccgcagcat cgtcatcatt catcgccttc ttgaaccctt
     2221 taatggccgc tccaaggtct ccgcccagcg tacgtaactt cttagtccca aacagcagaa
     2281 cgaccagcgc cgcaactacc agcagtttgg taatactaat ctcacccata gataccttct
     2341 tgacatataa ccggcaacag aacgcgcctt aaaaaccaga gaatattaac ggtcattcga
     2401 cgcgcgcaca caatagcaat ctgtaacaag gtgaatcaag cgccgagtaa aaaaacatca
     2461 taataattgc ggcggcgcaa agcgacggtt gtttaagacg ggcagttgcg cgcgcacctg
     2521 acgcacacgt tcgggcgtca cttccgccat aatgagtgca ggcatttctg acgctgccgc
     2581 aatggtgacg ccaaagggat ctataatccg gctttgcccg atatttttgt tcccgcactc
     2641 ccccgccgcc accatataac aggtggtatc cagcgcacga gcggcaagca acgttgacca
     2701 gtgatgctct ttgagcggcc cgcgaaccca ggcggcagga agtaccagga tttcagctcc
     2761 ctgtaatgcc tgtgccaacg ccagctctgg aaagcgtaag tcataacaga tcatcagacc
     2821 gaccttcatc ccttccacct ccagtaacgg agcgatttca ttaccagcat caacatggcg
     2881 tgattcctga atggcaaatg catcatagag atgcagtttg gcataacggg cgacgatgtt
     2941 tcctgcctgt agcgccacca gcatattcca tgcgcgcccc ggcgttgaag gaacatgaat
     3001 cgtcagaatt gtcgtcatca tgttacgttt actttctcgc cgtaaacgtc cgaggaattc
     3061 gccttccagc agctgtgctg atttaaccga tagatctgca tcatgatcat cgcgtgccag
     3121 caatgcttcc ggcaggacaa acagcgatac gtcgttttcc gccgcctgcg ccatcaacga
     3181 ggcacaaatc tcagcgttct tttcccacac agatgtaaca gcaaactgtc ctgcagcaac
     3241 cagcattgcc acctcctgtc agagtcgtaa aattcgccaa cgacgattat tcggcgttac
     3301 acttatcact catacaaatc aaatagcagg attttgcagt gttacaactt cttttagcag
     3361 tttttattgg cggtggtaca ggaagcgtgg cgagatggct gttaagtatg cgatttaacc
     3421 cgctgcatca ggcgattccg ttggggacgc tggcagcaaa cctgattggg gcattcatca
     3481 taggaatggg attcgcctgg ttcagcagga tgacgaacat tgatccagtg tggaaagtat
     3541 taatcaccac cggattttgt ggcggtctta caaccttctc aacattttcg gcagaagtgg
     3601 tgtttttgtt acaagagggc cgctttggct gggcgttact gaacgttttc gtcaaccttc
     3661 tggggtcttt tgccatgacc gcactggctt tctggctgtt ttcggcctca accgcacact
     3721 aaagaccaaa aaaaacccgc tgattaagcg ggttttgaat tcttgctgac gtatcttaca
     3781 gagcgattac gtttgcagca gaagggcctt tggcaccgtt agtgatttcg aactctacgc
     3841 gctgaccttc agcaagagtt ttaaaaccat tagtctggat tgcagagaag tgtacgaaca
     3901 cgtctttgct gccgtcttcc ggagtaatga aaccgaatcc tttggactca ttaaaccact
     3961 taacgttacc tttaatctta gacatcaaaa ttacctttac atgaaaaata gacacaaatg
     4021 ctgtgtcggt taccagtaca ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa
     4081 agtgataaaa gtcgcgttat ttttgatgtt acttatttta gttattgatt ttaaagaagt
     4141 tactaaaact tcatttgtct caaaactgaa agcgcatcca ggcaaagtac acattgccat
     4201 tgttgtaggt acccggaata taggtcatct gaaaagtcac tggaccataa cccacggagg
     4261 ccaatggcag tagaaccggg agagggatgt aattccagtt atcacgtgcc gttacgccag
     4321 cggtgaatcc cagacctaaa tgaaaatttt catccgccag cggtcgccag gtactttccc
     4381 atccgtatcc ggcaatcggt tcccatttgt tccacgagtc cttaaatgcc atggcataca
     4441 ggccatgcca gtttcctttt tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc
     4501 gctcgttata gcgatcggtt ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg
     4561 caggaatata taaatcataa tgttcaggct gttgccaggt ttgtgcaata ttttctctaa
     4621 acgttgtcat ccactcatct gcgttagcaa aagctttacc aacgctgatt aactgaataa
     4681 aaacaaagga aaagatagcg acatatttac tcacgttcat ttgt
//
LOCUS       DAZUCE010000304         4710 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZUCE010000304 DAZUCE010000000
VERSION     DAZUCE010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.306"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..270
                     /locus_tag="ACHY03_004740"
     CDS             <1..270
                     /locus_tag="ACHY03_004740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907877.1"
                     /translation="TTTMRTQKMNREKIEALMSVILKEADPKKFPEKLSEDTIVSFYR
                     ANSSYLHCPTLREWLSGEAEPSDEILEEIMIQNNWRATKWSNRLP"
     gene            602..799
                     /locus_tag="ACHY03_004741"
     CDS             602..799
                     /locus_tag="ACHY03_004741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907878.1"
                     /translation="MTYGKYKITLSKNNGSYIAKYINTETGDRDELSLSSRSLLLEAL
                     QSNLELDAMNISDLREDILAL"
     gene            complement(902..1336)
                     /locus_tag="ACHY03_004742"
     CDS             complement(902..1336)
                     /locus_tag="ACHY03_004742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907879.1"
                     /translation="MSCYRIKAAGENASALESVMDRVCNIAEKKNDKDVIILIAQKNY
                     GKKDKRLEQILGLTMYRTLMQDSAESVGIGDVSVQLKTFSYITNAAYRANNKTIVVVN
                     PSASDLEIKFKNLHKTTDVIAVEIHSDGELDGWVKDNQAVDL"
     gene            complement(1551..2030)
                     /locus_tag="ACHY03_004743"
     CDS             complement(1551..2030)
                     /locus_tag="ACHY03_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HFS3907880.1"
                     /translation="MTDPKWLIEARKNLGIREMKGKQHAAEIVQYWKDIKRGGIKDDE
                     TPWCAAFTGAMLERVGIRSTRFESANSYLDWGNELKEPAYGCIAILSRSGGGHVGFVV
                     GKNAAGDLMILGGNQADAVNIKAFPSSRVTGYRWPAGQTDVPQSLPLVNAEKSISEA"
     gene            complement(2030..2302)
                     /locus_tag="ACHY03_004744"
     CDS             complement(2030..2302)
                     /locus_tag="ACHY03_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFS3907881.1"
                     /translation="MTLHSVLINANAIICLMLALRLMFFQKTGRYRFFISLTAYLAIL
                     SAAWIALRILYGKYTQVDPAEFFLNLTICIAVWRARGNISKITGDR"
     gene            complement(2302..2685)
                     /locus_tag="ACHY03_004745"
     CDS             complement(2302..2685)
                     /locus_tag="ACHY03_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HFS3907882.1"
                     /translation="MSEPLTAGVAAGSAGVTFAALFPEATPAVMICALAGAALYVLSS
                     GQHRFWKQVIFALISFVGGVYCAETASAIITGILNAVLSHLNPPVTVKVSPAIGALVA
                     SVISVTSLLRIMSQARLWKSDKGMK"
     gene            complement(2798..3469)
                     /locus_tag="ACHY03_004746"
     CDS             complement(2798..3469)
                     /locus_tag="ACHY03_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFS3907883.1"
                     /translation="MSNEYLLEYTRIKLRAALRDLSGGSKGQLEALCEHPPADKNAYP
                     RKHIHRVQLEDRTVDALVTPVYALESFSRRRPAPPMNDFEFADSSWRRAVNSLDVSQQ
                     AWLRYCYGGHLAFKHQTAICEAVWSRYKGHIPASTQRKVVKRLLSLVWLSVQAVAAAN
                     KREDFKEMAGSALAGMLSVSRSTWCETYSQHWAGMKEAVRALDEMALLATLHHYQNHL
                     DDVCV"
     gene            complement(3469..3762)
                     /locus_tag="ACHY03_004747"
     CDS             complement(3469..3762)
                     /locus_tag="ACHY03_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFS3907884.1"
                     /translation="MKKIDLRKAARGRACTVRIPGVCNHNPETSLLAHYRLAGTCGTA
                     IKPHDMQGAIACSACHDAIDGRTKTDYEYDSLLLMHAEGVLRTLAIWRDEEFI"
     gene            complement(3759..4355)
                     /locus_tag="ACHY03_004748"
     CDS             complement(3759..4355)
                     /locus_tag="ACHY03_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFS3907885.1"
                     /translation="MAQHSFIRVSGGSLIPATPDTQRWLTERVKPGAVVYADFKQARN
                     PAFHRKFFSLLNLGFDYWHPSGGAISPTERELVHGYVKLLAYYGGHGDVMAELADQYL
                     LDESEKRAGNISAVKSFEAFRAWAIMEAGFYDVHQMPDGSLMRVPRSISFAAMDDLEF
                     GQLYSAVLDVLWNYILFRTFASQEAAENAAAQLLDYTS"
     gene            complement(4433..4612)
                     /locus_tag="ACHY03_004749"
     CDS             complement(4433..4612)
                     /locus_tag="ACHY03_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907886.1"
                     /translation="MNIQESIGELPETCRAVIKRKDGHIVGVRVLTDDERIASLMAFL
                     ELAEIAGYTITPPDA"
BASE COUNT         1315 a         1157 c          995 g         1243 t
ORIGIN      
        1 acaacaacca tgagaacgca aaaaatgaac agagaaaaaa ttgaagcctt aatgagcgta
       61 atattaaaag aagctgaccc aaaaaagttt cctgaaaagt tatcagagga caccatcgta
      121 tctttttatc gtgcgaactc ctcttaccta cattgtccaa ctttaagaga atggttaagc
      181 ggcgaagcag agccttcaga tgagatactt gaggagatta tgattcagaa caactggcgc
      241 gcaactaaat ggtcaaatcg gttaccttaa cctgttgaag gtgtggcgtc ttaaaacaaa
      301 aaggcggccg ctcgtttaag taaaacgtct cttacatcag gtaccagctc tttggcactt
      361 tttccaatga gcatagtaca cgattttttc tgtgcagatt tttacgcacg ctgcaaagtt
      421 gttattttat gtgccgctga acggtgcgta gttcagcttc agcgcttccc accggccttt
      481 aagagagata tctgatttag catgtcatgt gatgaacggt tactgcggag ttctccagtc
      541 cggttttgtc attcgtttgc ttcctataca atcatgcttt tgacactata agggataagc
      601 aatgacttac ggtaagtaca aaataactct atctaaaaat aatggcagtt atatcgcaaa
      661 atacatcaac actgagacgg gagatagaga tgaattatct ttatcatcac gttcgctatt
      721 gcttgaggca ttgcaatcaa accttgagct tgatgcaatg aatatatcag acctaagaga
      781 ggatatttta gccctctaag taaaaacttt tgttgagacg atgattgcca ttactatgct
      841 ccgatagtaa tggcaataaa aacctgctcc aaaaaacagt caaaacacac tttaacaacg
      901 gctataaatc tacagcctga ttatccttaa cccacccatc aagctcacca tcagaatgta
      961 tttccacagc gattacatct gtggttttat gcagattttt aaattttatt tctaaatcag
     1021 atgcgctggg atttacaaca acaatagttt tattgttggc tcgatatgct gcattagtga
     1081 tatacgaaaa ggtttttaat tgaacactaa catcccctat cccaacggac tcagcagagt
     1141 cttgcatcaa ggttctatac attgtcaacc ccagaatttg ctctaatctc ttatcttttt
     1201 tcccatagtt tttctgtgca attaaaataa tcacatcttt atcatttttc ttttctgcta
     1261 tattacaaac tcgatccatc actgattcta acgccgatgc attctctccc gccgctttga
     1321 ttctgtaaca actcatcacc ttctccattt atttatcggt tgacaacgaa ttagctcata
     1381 aataatagcc aaaaatcttt tacaagtcga taaaataaat attacttcac gctctcccta
     1441 tatgtttcac tgtcgtacga ttagcaagag tcgcttcggt gtatgtcgaa tgtgtgcagc
     1501 ctaccagcgt gaagaacgcg acagctaaga ggagtttttt cacattttgc ctacgcttct
     1561 gagatagatt tctcagcatt caccaatgga agtgattgtg gaacatccgt ttgacctgct
     1621 ggccagcgat agccagtaac gcgtgagctc ggaaacgctt tgatattcac tgcgtctgcc
     1681 tgattgccac ccaaaatcat taaatccccg gcggcatttt tcccgacaac aaaaccaacg
     1741 tggccaccgc cagaccgaga cagaatagca atgcatccat aggcaggttc cttcagttca
     1801 ttaccccaat cgagataaga attcgcagac tcaaaacgcg ttgagcgaat accaacacgt
     1861 tccagcattg ctccggtgaa ggcggcgcac catggtgttt cgtcatcctt aataccgccg
     1921 cgtttgatat ctttccagta ctgcacaatt tctgcagcgt gttgcttccc tttcatttcc
     1981 cggatgccga gattttttcg tgcctcaatt agccatttag gatcggtcat tacctgtctc
     2041 ctgttatttt tgaaatattt cctcgtgccc gccagacagc aatacagatg gtgaggttga
     2101 gaaagaactc tgcgggatca acctgcgtat attttccgta taaaattcgt agggctatcc
     2161 aagcagcaga cagaatcgcc aggtaagcag ttagtgagat aaaaaaacga tagcggcctg
     2221 ttttttgaaa aaacatcaac cgtagtgcca gcatcagaca gataattgca ttggcattga
     2281 tgaggacaga gtgcagggtc attatttcat ccccttatct gatttccata aacgagcttg
     2341 tgacatgatc cgcaacagtg atgtaacgct gattactgat gcaaccagtg caccaatggc
     2401 gggagagact tttactgtta cgggagggtt caggtgactc agcaccgcat tcagaatacc
     2461 ggtaatgatg gctgatgctg tctcagcgca atacacaccg ccaacaaacg aaatgagtgc
     2521 gaaaataacc tgcttccaga atcgatgctg cccggacgac aacacataga gagctgcgcc
     2581 agccagcgca cagatcatca ctgcaggtgt tgcctcagga aataacgcag caaacgtcac
     2641 ccccgccgag ccagcagcaa cgccagcggt tagaggttca gacatgattt ttcctgattc
     2701 agaaacgaca aaaccccgcc gtggcgaggt tcttagaaat ttggcaacat accaaattag
     2761 cgttaaatat ggtctatttt gttcggtttt gcaagcatta tacgcaaacg tcgtctaaat
     2821 ggttctgata gtgatgcaaa gttgcaagaa gtgccatttc atcaagtgct ctcaccgcct
     2881 ctttcattcc tgcccagtgc tgggagtatg tttcgcacca ggtggaacga gaaacagaca
     2941 acattccagc tagcgcagac ccggccatct ccttaaaatc ctcgcgttta tttgctgctg
     3001 caaccgcctg cacggacaac cacaccaacg aaagcaggcg cttaactact tttctctgag
     3061 ttgacgcggg gatgtgtcct ttatagcgac tccagacagc ctcacaaata gctgtttggt
     3121 gtttgaacgc caggtgacca ccgtagcaat agcgcaacca cgcctgctgg ctaacgtcga
     3181 gcgaattcac ggcgcggcgc cacgatgaat cagcgaattc aaaatcattc atcggcggcg
     3241 cgggacgacg tctgctgaaa ctttccaggg cataaactgg cgtaaccaga gcatcaacgg
     3301 tccggtcctc cagttgcaca cgatgaatat gtttgcgtgg gtatgcgttt ttatctgctg
     3361 gcgggtgctc acacaatgct tccagttgcc ccttagaacc accagacaaa tcccgtaatg
     3421 cagcgcgcag ttttatgcgg gtatattcca ataaatactc gttactcatc agataaactc
     3481 ctcatcgcgc cagatagcca gtgttcttaa aactccctca gcgtgcatca acaacaatga
     3541 gtcgtactcg taatccgttt ttgtacgtcc gtcgatagca tcgtgacacg cactacaggc
     3601 gatagcgccc tgcatatcgt gaggtttgat ggctgtgccg cacgttccgg cgagacgata
     3661 atgagccagt aagctggttt caggattgtg attgcacaca ccggggatac gcacagtaca
     3721 agcgcgacca cgtgcagctt ttctcaggtc gatttttttc atgatgtgta atccagcagc
     3781 tgcgcggcag cattttcagc ggcctcctga gaggcaaacg tgcggaacaa aatataattc
     3841 cacagcacat ctaaaacggc tgaatacagt tgaccgaact caagatcgtc catcgcagca
     3901 aacgagattg aacgaggtac gcgcatcaaa ctgccgtctg gcatctgatg aacgtcataa
     3961 aacccagctt ccataatcgc ccatgcgcga aacgcctcga acgacttaac cgcactgata
     4021 ttccccgcgc gcttttccga ctcatcgaga agatactgat cagccagctc tgccatgaca
     4081 tcaccgtgtc caccgtaata cgccagtaac ttaacgtagc catggaccag ttcgcgctcc
     4141 gtaggagaaa tagcccctcc ggacggatgc cagtaatcaa agcccaggtt tagaagtgag
     4201 aaaaatttac gatgaaacgc gggattacgc gcctgtttga aatctgcata cacaacagca
     4261 cctggtttga ctcgttcagt caaccagcgt tgcgtgtctg gtgtcgcggg tattagcgaa
     4321 ccgccggata ccctgataaa tgaatgctgc gccatggtta actccaggtg gcgcggcagt
     4381 gttcagaaaa tggtgccggg tgttcaatcc agcaccgata ttataccgtg ttttacgcgt
     4441 cagggggtgt aatagtatat ccagctattt ctgccaactc gagaaacgcc atcaggctgg
     4501 caatccgttc atcatcagtc agaacacgca caccaacgat gtgtccgtcc ttacgcttga
     4561 taacagctcg gcatgtttcc ggtagttcgc ctatagattc ttgaatattc atgtcttccc
     4621 cctaacgcca ttacctgtat atttatacag tattttatgg catttgtcat aacgccagat
     4681 acaatgaggg aataatgaac aattatattc
//
LOCUS       DAZUCE010000305         4603 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZUCE010000305 DAZUCE010000000
VERSION     DAZUCE010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.307"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            229..1386
                     /gene="acrE"
                     /locus_tag="ACHY03_004750"
     CDS             229..1386
                     /gene="acrE"
                     /locus_tag="ACHY03_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFS3907887.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEVR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1398..4502
                     /gene="acrF"
                     /locus_tag="ACHY03_004751"
     CDS             1398..4502
                     /gene="acrF"
                     /locus_tag="ACHY03_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFS3907888.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1118 a         1112 c         1200 g         1173 t
ORIGIN      
        1 ttcaatttac gattttttaa ttctgcctct ttcaacccgc gtcaaaataa aacagtagaa
       61 tattaatctt tttttgtgtt tatgtgcctt gagacgcctg tattcataac tattccttac
      121 atcgacgaat gataatttgt aggatagcga actgtatttt tctttctgcg agttaacgcg
      181 ttgccttttt gggtaaataa cgcgcttttg gttttttgag gaatagtaat gacgaaacat
      241 gccaggtttt tcctcctgcc ctcctttatt ctgatctccg cggctttaat cgccggttgt
      301 aacgataagg gagaagagaa agctcacgtc ggtgaaccgc aggttaccgt tcatattgta
      361 aaaacggccc cgttagaagt taagactgaa ttaccaggcc gcaccaatgc ttatcgtata
      421 gccgaagttc gcccacaggt tagcgggatc gtactgaatc gcaatttcac tgaaggcagc
      481 gatgtgcaag caggccagtc cctgtaccag atcgatcccg cgacctatca ggcaaattat
      541 gacagcgcga aaggcgaact ggcgaaaagt gaagccgccg ccgccatcgc gcatttgacg
      601 gtaaaacgtt acgttccgct cgtgggtacg aaatacatca gccagcagga gtacgaccag
      661 gccattgctg atgctcgcca ggccgatgcc gccgtgattg ccgcaaaagc cacagtcgaa
      721 agcgctcgca tcaatcttgc ttataccaaa gtgactgcgc caattagcgg acgtatcggc
      781 aaatcgactg tgaccgaagg cgctcttgtc actaatgggc aaacgactga actggcaact
      841 gtccagcagc tcgatcctat ctatgttgat gtgacccaat ccagcaacga ttttatgagg
      901 ctgaagcagt ccgtagagca aggaaatttg cataaggaaa acgccaccag caacgtagag
      961 ttggtcatgg aaaacggtca aacctatccc ctgaaaggta cgctgcaatt ctccgatgtg
     1021 accgtcgatg aaagcaccgg ctccataacc ctacgtgctg tcttccctaa cccgcaacat
     1081 acgcttttgc cgggtatgtt tgtgcgtgca cggattgatg aaggcgtcca acctgacgcc
     1141 attctcatcc cgcaacaagg cgttagccgc acaccgcgtg gtgatgcaac cgtgctgatt
     1201 gttaacgata aaagtcaggt ggaagtgcgc cctgtcgttg ccagtcaggc gattggcgat
     1261 aaatggttga ttagcgaagg tctgaaatct ggcgatcaag tcattgtcag cggcctgcaa
     1321 aaagcgcgtc cgggagagca ggttaaagcc actaccgata cccccgcaga tactgcatcg
     1381 aagtaaggta atctgacatg gcaaactttt ttattcgacg accgatattt gcatgggtgc
     1441 tggccattat tctgatgatg gcgggcgcac tggcgatcct acaattgccc gtcgctcagt
     1501 atccaaccat tgcaccgcct gcggtttctg tttcagcaaa ctatccgggc gctgatgcgc
     1561 agaccgtgca ggatacggtg acgcaggtta tcgaacagaa tatgaacggt atcgataacc
     1621 tgatgtatat gtcctccacc agcgattccg ccggtagcgt gacaattacc cttaccttcc
     1681 agtccgggac cgatcctgat atcgcgcaag tgcaggtgca gaacaaactc cagctcgcca
     1741 cgccgttgct gccgcaggag gttcagcagc aggggatcag tgttgaaaag tccagtagca
     1801 gctatttgat ggtggcaggc tttgtctctg ataacccaga caccacacag gacgatatct
     1861 cggactatgt ggcctctaac gttaaagata cgcttagccg tctgaatggc gtcggtgacg
     1921 tacagctttt cggcgcacag tatgcgatgc gtatctggct ggacgccgat ctgctaaaca
     1981 aatataaact gacaccggtt gatgtgatta accagttgaa ggtacagaac gatcagatcg
     2041 ctgccggaca gttgggcgga acgccagcgt taccagggca acagttgaat gcctcgatta
     2101 ttgctcagac gcggttaaaa aatccggaag aattcggcaa agtgaccctg cgcgtaaaca
     2161 gtgacggctc ggtggtgcgt ctgaaagatg tcgcacgggt tgaacttggc ggtgaaaact
     2221 ataacgttat cgctcgcatc aacggaaaac cggcggcggg cctgggtatt aagctggcaa
     2281 ccggcgcgaa tgctctcgat accgcgaaag ccattaaggc aaaactggcg gaattacagc
     2341 cattcttccc gcagggaatg aaggttctct acccttatga caccacgcca ttcgtccagc
     2401 tttctattca cgaagtggta aaaacactgt tcgaagctat tatgctggtg ttcctggtga
     2461 tgtatctgtt cttgcagaat atgcgagcaa cgctgatccc caccattgcg gtacccgtgg
     2521 tgttgttagg gacgtttgcc atcctcgccg cttttggtta ctccatcaac acactaacga
     2581 tgttcgggat ggtgcttgcc atcgggctgc tcgtcgatga tgcgatagtg gtggtggaga
     2641 acgtcgagcg cgtgatgatg gaggataagc tcccgccaaa agaagcgacg gaaaaatcaa
     2701 tgtcgcaaat acagggcgca ctggtgggta tcgcgatggt gctgtcagcg gtatttattc
     2761 cgatggcatt cttcggcggt tctactgggg caatttatcg tcagttctct atcactatcg
     2821 tttcggcaat ggcgctttct gttctggtgg cattgattct tacccctgcg ttatgtgcaa
     2881 cactgcttaa acccgtctct gctgagcatc acgaaaataa gggcggtttc ttcggctggt
     2941 ttaataccac cttcgatcat agcgttaacc actacaccaa cagcgtcggc aaaatcctcg
     3001 gttccacagg acgatattta ctgatctatg cgctgattgt tgcaggaatg gtggtgttgt
     3061 ttttacgtct tccgtcttcc ttcttacctg aagaggatca gggtgtcttt ctgaccatga
     3121 ttcagttacc cgctggcgcg acgcaagagc ggacgcaaaa agtgttggat caagtgacgg
     3181 attactatct gaagaacgag aaagcaaacg ttgaaagtgt ctttacggtt aacggcttta
     3241 gcttcagcgg ccaggcacaa aacgccggta tggccttcgt cagtctgaaa ccgtgggaag
     3301 agcgtaatgg tgacgaaaac agtgcggaag cggtaatcca tcgtgccaaa atggaattgg
     3361 gcaagatccg cgacggtttt gtcattccat tcaacatgcc agccattgtt gaactgggca
     3421 cggcaacggg tttcgacttt gagttaattg atcaggctgg gctgggtcac gatgccctaa
     3481 cccaggcccg taaccagttg cttggtatgg cggcgcaaca tcctgccagc ttagtcagcg
     3541 tgcgtcctaa tggcctggaa gacaccgcgc agtttaaact ggaagttgac caggaaaagg
     3601 cgcaggcatt aggtgtttca ctttctgaca tcaatcagac catttcaacg gcgttgggtg
     3661 ggacttacgt taacgacttc atcgaccgtg gtcgcgtgaa aaaggtgtat gttcaggcgg
     3721 atgccaaatt ccgtatgctg ccagaagatg tcgataaact ttatgtccgc agcgccaacg
     3781 gcgaaatggt gccattctct gcctttacca cttcacattg ggtgtatggc tctccgcgac
     3841 tggaacgcta caacggtctg ccgtcaatgg agattcaggg ggaagccgcg ccaggaacca
     3901 gttccggcga tgccatggcg ttgatggaaa acctagcgtc aaaattacct gcgggcattg
     3961 gttatgactg gacgggtatg tcgtatcagg aacgcttatc gggaaaccag gctcccgctc
     4021 tggtagcaat ttcctttgtg gttgttttcc tgtgccttgc tgcactctat gaaagctggt
     4081 caattcctgt ctcggttatg ttggtcgtgc cgttagggat tgtcggcgtg ctgctggcgg
     4141 cgacactctt taatcaaaaa aatgacgtct actttatggt gggcttgcta acgacaattg
     4201 gcttgtcggc caaaaacgct attttgattg ttgagttcgc taaagatctc atggagaaag
     4261 agggtaaagg tgttgttgaa gcgacactaa tggcagtacg tatgcgtctg cgtcctatcc
     4321 tgatgacctc tctcgctttt attctcggcg tattaccgct ggctatcagt aacggtgccg
     4381 gcagtggcgc gcagaacgca gtgggtatcg gggtaatggg cggaatggtc tctgcaacgt
     4441 tgctggcaat cttcttcgta ccagtgttct ttgtggtgat ccgccgttgc tttaaaggat
     4501 aaatctgaaa cataaaggcg ctttcgggtg cctttattat tttcagtgaa acccataaaa
     4561 attaataaga tattcttttg ctcactcttt aaaagctttc tat
//
LOCUS       DAZUCE010000306         4592 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZUCE010000306 DAZUCE010000000
VERSION     DAZUCE010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.308"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(4..1106)
                     /locus_tag="ACHY03_004752"
                     /pseudo
     CDS             complement(4..1106)
                     /locus_tag="ACHY03_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
     gene            1135..1410
                     /locus_tag="ACHY03_004753"
                     /pseudo
     CDS             1135..1410
                     /locus_tag="ACHY03_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419664.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2183..3160
                     /locus_tag="ACHY03_004754"
     CDS             2183..3160
                     /locus_tag="ACHY03_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFS3907889.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3446..4186)
                     /locus_tag="ACHY03_004755"
     CDS             complement(3446..4186)
                     /locus_tag="ACHY03_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3907890.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGLRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALVGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4307..4537)
                     /locus_tag="ACHY03_004756"
     CDS             complement(4307..4537)
                     /locus_tag="ACHY03_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFS3907891.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWYYPNSP
                     KNDKTALHPLHYWLHEGSEQKKNPFPIWFYPR"
BASE COUNT         1127 a         1146 c         1078 g         1241 t
ORIGIN      
        1 tatttaaatg ccccagaact tattcgcttt ttccacaagc gtttcatcaa tcagtgcacc
       61 ggtgacagcc atcagtaaac catacccgat aatgcctaaa gcgcttccgg taagaatact
      121 gaagaccaga gcaacaagag ccgttgcggc attacctgct atgatggttt ctgttttaac
      181 aaaaaaggac gccagttatc tgtccgggca gccttaccaa actcagtgat ccagtcagca
      241 aaatttgtaa tttttcctgc atatcccagt ccgcgactga acctgttcag attagacgat
      301 atatcagaca gcttcacaga ctcaagggct gcggcaatcg ctgcacgatc ttttgcatta
      361 atttttttgt taatgtcagt ccggtacttt tcatacgttt ttaatgcctc ttcaacatta
      421 cgtattttct tccctttagc ctgcccggcc atctctctgg ctaactgctc agcttttgca
      481 ccatattttt ctgaaactga tttcaggaac tctgttgtga aattaattgc gtctttcgtt
      541 gccttcagtt catcctgttt tcttttctct tccgctatct tctgattgat gccggcaagc
      601 tggttacggg tattctcttt ttccttcaac agagcattaa gagcgtcatt tgcatgcttt
      661 tgctcatttg ttctggcact gaggttattt ctcaccgaat ttaccgcaga ttcagcagag
      721 gtgattttat ttctggcatc aagcaatctt tgccgtaact tatcccattc agtaacttca
      781 gcattataaa ttttcttgtc accttccaca tcctctgtca ccaccggatt ccggtcattc
      841 agaatattgc gtttatagtc cagaccggaa tgagtcagaa gataattcag gtttgcacgg
      901 ttatcgatga ctgcgctggt tgtgattttt atcgtatcgg caaatctcgg atcaccgctc
      961 acaacaatag aatgatttgt tgatgaacgc cccgggaatt tttcagagga aactatcgtt
     1021 ttgccgtcag gcgtgttttt ctgttgcgtc agctgttgtt cagctctggt taatgcatta
     1081 cgggccgtgt taagcgattc cgtcaccgaa ctgagtttat tccgggcctc tgaaatggcc
     1141 ctgtcattcc gtgtctgctg ctcgagcaga cgggcctgtt gtgcatcaag aacggcaagg
     1201 gctgccccgc cctttttttc cagctctgac aaccgcgtat tcagaatacc ttttctgcgc
     1261 tcgtattcag caagcatatc agcggccttc ttgtctgcgt tctgcgcatc cttctgccgt
     1321 ttttcagcct gttcaattaa cgacctggcc tcgcggggag atatacgaag agaatcagca
     1381 tcagcaaaca gcatccggct ctctgtatgt gtcaacgcca cgatgtttga ccgtcttata
     1441 tcactggcgc tgacaacacg acagagttat caggcatggt gtcaatcagt tcacaatttt
     1501 tccatcggag cagcctacca ccggtgataa cctgactttg cctgtagttt gtatacacaa
     1561 tccctggcat tgaaacacgc atatgaggtg tacttcggaa tatgaccgtg cgctcaggaa
     1621 gtacggcaga ttccgtcaaa aattacctgt aaagacagac ttcagaaaaa acagtgctcg
     1681 ccgcagaccg gtgaccgggc gaagcccgcg aaccggcgag gaagcgcaat atatctgtag
     1741 ttacaatgag caggtacgcc ctgaagcctt cataatcggt agtgtatgtt gtttttctgg
     1801 aatgatttat ccacatatct ggaggtccga tccagtaaac agatccatga atgatcaaca
     1861 aaggatccat taaagatccc catatcgctg caaaccttgt cactcatggg ccgggaccac
     1921 gatcacataa gctgtggcat gttactgata aactgtaaca tgctaatgat aagctgtatt
     1981 cagtaatcca tatactgaag taagttaatg acataaacta tggtcagtac gtcagactca
     2041 gttgttaaat acaggctgca ggtttttatt cagtcagtta gcggagttct gacacacgat
     2101 tttctgttta ttcttttact gtccacaggc tggaggcttt ctggaaaacg aaaattcaga
     2161 catcaaaaaa ctgttcggcg aggtggataa gtcgtccggt gagctggtga cactgacacc
     2221 aaacaataac aacaccgtac aacctgtggc gctgatgcgt ctgggcgtct ttgtaccgac
     2281 ccttaaatca ctgaagaaca gtaaaaaaaa tacactgtca cgtactgatg ccacggaaga
     2341 gctgacacgt ctttccctgg cccgtgcaga gggattcgat aaggttgaga tcaccggccc
     2401 ccgcctggat atggataacg atttcaagac ctgggtgggg atcattcatt cctttgcccg
     2461 ccataacgta attggtgaca aagttgaact gccttttgtc gagtttgcaa aactgtgtgg
     2521 tataccttca agccagtcat cccgcaggct gcgtgagcgc atcagtcctt ccctgaagcg
     2581 cattgccggt accgtgattt cgttttcccg taccgatgag aagcacaccc gggaatatat
     2641 cacccatctg gtacagtcag cctactacga tactgagcgg gatattgtgc agttacaggc
     2701 cgatccccgc ctgtttgaac tgtaccagtt tgacagaaaa gtccttctcc agcttaaggc
     2761 gattaatgct ctgaagcgac gggagtccgc ccaggcactc tacaccttta tagagagcct
     2821 gccccgggat ccggcaccga tatcgctggc gcggctgcgt gcacgcctca atctgaagtc
     2881 tcctgtattt tcccagaacc agacggtcag acgggcaatg gagcagttgc gcgagattgg
     2941 atatcttgat tacacggaga tccagcgggg gcggacaaaa ttcttctgta ttcactaccg
     3001 gcgtccccgg ttaaaagcgc cgaatgatga gagtaaggaa aatccgttgc caccttcacc
     3061 tgcggaaaaa gtcagtccgg agatggcgga gaagcttgcc ctgcttgaga aactgggcat
     3121 cacgctggat gacctggaaa aactcttcaa atcccgctga atataaactg tagtcagtga
     3181 agagcgctcc tttactgact acagcttata ttaactggtg cagtgagtgg tctgctcact
     3241 gcagtttata ttcttgttcc ctgcagtgct gtttgtagct gagctgccat ctgcctgtcc
     3301 cttacgtaag tgatcccgta acctgatgct gaggcattgc tcccttcata aaacatgact
     3361 tactcactac agcttatata catgctccag cttatgttat gtctgttctg ctgaccacag
     3421 cttatataag gaagcgcgta tgccgtcacc tcaggggagg cagtgtacgc aggatctccg
     3481 cagcatcccg cccgtcaccg gtgaaaggca ctgccagcgt ggcagccata tccagcgcaa
     3541 acactctggt ataaacctcc atcgaacgtg gatccctgtg accgaccagt gcctggatga
     3601 ctttccgggg ctggcggtga tagagcatgt gcatgatata gctgtgccgg aaggtgtgtg
     3661 gtgtgaccgg aatcgaaaag tgaaccccgt ctgcttcggc ccgtctgaca gcctgcttca
     3721 gccagttgcg catggtttcg tcggtcacgg cccataatgg ttcacgacgc cggggccggg
     3781 tggtgatcat ccagctttcc atctgcctga catagcttat atctgtcagc ggaaccaggc
     3841 ggacttcatc ttttggcggg cgtccgcgtc gcgcacgcac tttttcggac aggatccgca
     3901 caaacggtcg cagaccatcc agatcaaatg attccggcgt cagcatccgg gcttcgccaa
     3961 tacgcattcc ggtattccag agggtggcaa acagcatatg gtgacgctga tccggcatat
     4021 agaagagaag ggcactgact tccggagcca gcaggtaggc cggtgtggcc cccgtggaag
     4081 tggccattct tctcagggag agcgctgttg caaaatccac ccccggcgca atgggtaaca
     4141 gggagacgcg ttctggtgaa ttctgcaggg gaatgacatt gttcatgagt tatttttcac
     4201 gtagcacacg cactgtttat ggatacaggt attctgacct gaacgcccgg atccttccac
     4261 gacaataaat cgcagcgcgc agatcctttt gtttgcttat cccacattat cgcgggtaaa
     4321 accaaattgg aaaaggattc tttttttgct cagatccttc atgaagccag taatgaagcg
     4381 ggtgtagggc tgttttatcg tttttgggag aattggggta ataccaggac gctaatgatc
     4441 tatcaatgga tggatgtaag tgacaattga aaactgaaaa atgcgctatg tagattctga
     4501 cagatttatg cacctgaaat acgttcaaaa tccacatagc gtaactctga ttttccttac
     4561 cagaaaaaag agggggaata accagtatct cc
//
LOCUS       DAZUCE010000307         4579 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZUCE010000307 DAZUCE010000000
VERSION     DAZUCE010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.309"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..348
                     /locus_tag="ACHY03_004757"
     CDS             <1..348
                     /locus_tag="ACHY03_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907892.1"
                     /translation="SVTQRLLPTPLAAVRARRVAFENPYRNKVLSESLPAGNLLYRSA
                     LAFIQALIALVPSKKILDLDKITDALRRRYVHLEQLEYTELRNALACALSTCYNRKYL
                     IKVAKGKYLPVDD"
     gene            430..777
                     /locus_tag="ACHY03_004758"
     CDS             430..777
                     /locus_tag="ACHY03_004758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3907893.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            950..1114
                     /locus_tag="ACHY03_004759"
     CDS             950..1114
                     /locus_tag="ACHY03_004759"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3907894.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(1118..1306)
                     /locus_tag="ACHY03_004760"
                     /pseudo
     CDS             complement(1118..1306)
                     /locus_tag="ACHY03_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1367..2491
                     /locus_tag="ACHY03_004761"
                     /pseudo
     CDS             1367..2491
                     /locus_tag="ACHY03_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
     gene            complement(2488..3644)
                     /locus_tag="ACHY03_004762"
     CDS             complement(join(2488..3395,3395..3644))
                     /locus_tag="ACHY03_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFS3907895.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDIFKKSNSVFCTGVAEKYALI
                     EQWRQQFPIEAMCQVFGVSKSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGT
                     RRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFA
                     PTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALR
                     SQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE
                     SLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            complement(4020..4298)
                     /locus_tag="ACHY03_004763"
                     /pseudo
     CDS             complement(4020..4298)
                     /locus_tag="ACHY03_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4425..4511
                     /locus_tag="ACHY03_004764"
     CDS             4425..4511
                     /locus_tag="ACHY03_004764"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3907896.1"
                     /translation="MVKLALQLGASVARIAQEHDINDNLLFK"
BASE COUNT         1219 a         1076 c         1066 g         1218 t
ORIGIN      
        1 tcagttactc aacgattgtt acccacacca ttagctgcgg taagagcccg aagagttgca
       61 tttgaaaatc catatagaaa taaagtacta agtgaatcat tacctgctgg aaaccttctt
      121 tacagaagtg cattagcttt tatacaggcc ttgattgcat tagtcccatc aaagaaaata
      181 ctggacttgg ataagataac agatgctctg cgtaggcggt atgttcatct tgagcaatta
      241 gaatataccg aattaagaaa tgctttagct tgtgcattgt caacttgtta taatagaaaa
      301 tatctcatta aagttgcaaa aggaaaatat ttacctgttg acgactaggt gcgtattacc
      361 gtgaaggaga tcggtgagta accccctgtg tcagcatagt tgtcgcctca attttttaag
      421 gagataacaa tgccaggaca accaatacca agtgagctca aagagaaagg actgatatgg
      481 cttttaccac catactgctg gtcacatcgt cagatagccc gtaagctaaa gatcagtgcc
      541 tcagttgtat caaaatggag atatgagtta gtggagcaag ggcttttacc tgaaaatgaa
      601 aagattacct ccgttgatgt tgaagactgg acaccggagc gtcgattctc agttgttctt
      661 gagacggcta cgctgtcaga aatggagctt gctgattatt accgacgcat gggattgttt
      721 gttgaacagg ttaaagaatg gcgggctatc tcaattcagg cgcatgaaaa aaattgacgg
      781 aaaatattcg aacagacagg gcattgcgtg acgcaaaaaa taagttacga gaactgtaga
      841 aagagcttaa gcgtaaagat aaagctctgg cggaagctgc ggccctgctt atattgaggg
      901 aaaagttcaa tgccctctgg gacaacagcg aggaagactg acccctctcc tggagcggcg
      961 taaaattatt gcccttgtcc gcgaggccgt ctccaacggc gcccggaaaa gtcaggcttg
     1021 taggtgtatc ggtatttcaa ttcggacact tcagcgctgg actgatggca acgttattac
     1081 cacggatgta agcgtcaacg gagcaccgta ttgacgctta tttattggtg agtactacgt
     1141 tccatggcag gagttcatca actcggttgg aaggccattc cggcagtacg ctcaggataa
     1201 ggcgcagata cgcttccgga tcgataccgt tcagacggca ggtgccgatc agcccgtaca
     1261 acagtgctcc acgctcgccg ccgtgatcgc tgccaaagaa cataaagatg gtatgacctt
     1321 tatatactca ctatagcatt ggtgggatat tccagaggag gtcattatgc attcggaaaa
     1381 tattgcagct tacatcgggc tggatgtaca taaagaaacg ttggcggttg ctattgctgc
     1441 acctgaacgg cttggcgaag ttcgttatta tggtacgatt aacaacgaag cccaggctgt
     1501 acgtcgatta tttcagaaat tgcagggact ttacggaaac attctttcct gctatgaggc
     1561 tggcccctgt ggttttgggc tatatcatca actcacagcg atgaatataa aatgccaggt
     1621 tattgcccct tcccggatac caaaatcacc gacagacaga atcaaaaatg atcatcggga
     1681 cgcaatttct cttgccaggt tactgcgggc tggtgaactt acccctgtct ggatccctga
     1741 cctgactcat gaagctatgc gggatctaat cagagcccgt gcagcagcta aacgagacag
     1801 cagagttgcc cgacagcgaa ttctgagcat gctattgcga acagacaaac gttatgcagg
     1861 gaagcactgg accggcaaac atcgaacctg gttagccaat caatcattta gtcagccatc
     1921 tcaacagatt gcttttcaac actattgtca gagtctggag cagattgaag atcgcatact
     1981 ttaactcgac caggaaatat cccgcctgtt acctgagtgg tctctgtgca atctggtctg
     2041 ccagttacag gcccttaaag gtgttggtca gctaaccgca ataaccctgg ttgctgaact
     2101 gggtgatttt tcacgatttt ccagtcccaa acaactcatg gcttttctgg gactggttcc
     2161 aggggaatat tccagtggaa acagcattcg tcccagggga ataacaaaag ttggaaacag
     2221 cgaactgaga cgtctgcttt acgaagccgc ctggtcttat cgtacacctg caaaagttgg
     2281 tgcatggctt atatattacc gaccggattc tgtaacacaa tattccaaag atattgcatg
     2341 gaaagctcaa caacgattgt gttctcgtta ccgaagtctg acagcaaaag ggaaaaaatc
     2401 acaagtagcc attacggcgg tggctcgtga gttgactgga tttatgaggt gtactggcaa
     2461 tagcggacac taccatttgt tcttttttta agcagccatc tgatgatatt tttccctgaa
     2521 ggctgccggg gagatattcc ccagacgaga gtgacgacgc tgacgattgt agaaaatctc
     2581 aatgtattcc cgtattactg agatggcttc atcccggtta ttaaaacgat agtggctcag
     2641 gctctcattt ttcagcgttc cccagaagct ttccatcgga gcgttgtcgt aacagttacc
     2701 tttacgcgac attgatgttt tcagaccaaa ctgctcctgt atgacccggt aatcgtatgc
     2761 gcagtactgt gaacctcgat cagagtggtg gattagcccg gcaggtgggc gctggctcct
     2821 gagcgccata aacagggctt tacctgtcag ctcttttgtc atgcgctctc ccatggcgta
     2881 gccgacaatt tcgcacgtat aaacatcttt gatgccagcg aggtacaacc atccctcctg
     2941 tgtggcaaca tacgtcaggt ccgccaccca gacctgattt ggtgctgtag gagcgaacgt
     3001 ctggttcagc agatttggcg caactggcag attgtggttc gagttcgtag tcgctctgaa
     3061 cttgcgtttc tgcttacagc gtagccttag ctccttacga agacgtgcca gtcggtcacg
     3121 accaacgatg atgccattct ctgccagctc cgtctggagc cgccgggttc catatgtttc
     3181 gcgagtgcgg atatgtgcca ccttaatctc cagttttagc cgctcatcac tttgttttct
     3241 gtctgagggt tcatgctgta cccagttgta ataaccgctc ttggatacac caaatacctg
     3301 acacatcgct tcaatgggaa attgttgtcg ccattgttcg attaacgcgt atttttcagc
     3361 gactcctgtg caaaatacgc tgttgctttt tttaatatat ctcgctcaag gcgagcttca
     3421 tttaacgcct tacgcagttg cagaatttca gattccagtt cagccaccgt gcgggaacca
     3481 ggagttccga gcccttttct ggcggcggta acccattgtc ctaaagtgcc ttcaggaaga
     3541 gataatcggg aagcgccttc actgatcgaa agttgatttt caagaaccgt tctgacagct
     3601 tcggctttga actctttaga gtaacgttgg gtttttctgc tcattattag ctccttctga
     3661 tgccattcta tttcaggaag gagtgtccgt taaactcagg ctacctcatt atgtgggata
     3721 ttgcacttgc tgcccaatca tcattcagtc agcagaagca aaattaaacc ctgaagcagt
     3781 cagacacgga atgagtgatc ctcgtaaaag aattcaggag gtcagaatgc gccaatacct
     3841 gcatatagaa agaggccagc tcactgcgta aatcgtcatg gggctaaaat ccccgaataa
     3901 gagtttgtaa aatccaagat acagtctgac tgtttcaggt tctttaatcc gcgatataac
     3961 gaagctcccg gagctgcgta aggtcttttt gggctcaata tttgacaaag gtcataagag
     4021 tttttctttc cgagacagac tgcacgaagc gctctttccg cagcattatt atccgcctcc
     4081 gccagaccgt catcactgta ataacagagg gcatcccact gattcagtac atagctgaac
     4141 gcttcgccca gtctggattt tttcgacagc gtaccattct tctccaccat ccattcatgc
     4201 agcgacgtca gtaacacttt gcttcgctgc tgcctgacgg aaagacgctc tgactccggt
     4261 aatccccgta tttcgtaagc gtaaaccgac cgccgtatgt agccattaag ccagtattgg
     4321 taacgtaggt gcccaccttt tcaaactagt ggacgcctgt tatggagcaa aaaacattat
     4381 ccgcagaacc ccgcagatca ttttcaaata attttaaact tcaaatggtt aaactggctt
     4441 tgcaactagg agcctctgtt gcccgtatcg cccaggaaca cgatatcaat gataacctcc
     4501 tgttcaaata gctcagactc tggcaaaacg aagggcgtat atctcggcgt ctttcggtaa
     4561 caacctcttc tgacactgg
//
LOCUS       DAZUCE010000308         4578 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZUCE010000308 DAZUCE010000000
VERSION     DAZUCE010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.310"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(541..1002)
                     /locus_tag="ACHY03_004765"
                     /pseudo
     CDS             complement(541..1002)
                     /locus_tag="ACHY03_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1310..1423
                     /locus_tag="ACHY03_004766"
     CDS             1310..1423
                     /locus_tag="ACHY03_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFS3907897.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
     gene            complement(1667..2371)
                     /locus_tag="ACHY03_004767"
     CDS             complement(1667..2371)
                     /locus_tag="ACHY03_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HFS3907898.1"
                     /translation="MRRLPLMVGVIVGVMLAVGCHDRRAGVKTLLGDGMDQWGQKLPY
                     DHWQFNFFYPKNLPALVTMVYLEDGDVRESIFRQLDAARPSQSSVDTWSQRVGSFSAS
                     FNIGKALPVRMTVCWDSVIDKKAYETEIWFSRDTWQQMLTAYPDTYKPGKTYYRDNMI
                     IGLAPGGAVRIWLEDNGEPVVLQHPARQFTLTGDDMLICKNVPNQIDFSYIKANGYDP
                     FIRDFIKEKPYPYGHW"
     gene            complement(2382..4073)
                     /locus_tag="ACHY03_004768"
     CDS             complement(2382..4073)
                     /locus_tag="ACHY03_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFS3907899.1"
                     /translation="MVEEDEKNVPGDTCYYRYEIIGDSTHRTSIHNSFKTIQCRRVPV
                     RGITLTVGVFFDGTGNNRANADDLRLAYAHCANLVDEERARACEKYEKRARAGLGNAS
                     WQGGHTNISRLFDLYQQSDELNDSETEAQVKAYISGIGTADGESDSILGKALGSSLIR
                     QFEGVVTKTDEALEKISAELTRFIKLNRDKVAIAKVQFDVFGFSRGAAAARHFANRVM
                     NQDAAIAAAIDKGTDLTGHHGKLAGEVRFLGLFDTVAAIGSLLNFYDVNGRSNPGVNL
                     ELRPSVAQKVFQISAMHECRYNFSLNSIAGMWPELALPGAHSDIGGGYNAGEDEISLL
                     TMPDFEVTRDSADEMQTEVYRKAEKMRQVLYSLPALKYLMPHGEVETKMISWPLVNQN
                     KARASIFEKKVGAAVFMTRRDVPNDWEKVSLRVMLDAAQDAGAVFTPILSSNKELALP
                     SELLPLCDKAIAQGRAVRRGAEPEGFTAEDMRTIGRYLHCSANWNIDSDLSLWVSPSS
                     GEVFLRNNYAPTDERSFVWPMAPGKGWVRTVWRMDDQQQWEERARVNADALDGLF"
BASE COUNT         1064 a         1341 c         1010 g         1163 t
ORIGIN      
        1 ttcacttcgc agcaaaaaac gaaattctac aatttttctg attttactga cagaaaagcc
       61 tgagagttaa atttaaataa cttaaaagat tatgtggtat aatcaggttc atacaggcct
      121 gattatttta cggggatgtt aactctttca aatcaacagt ctacggagaa gcaatgtggt
      181 tatggtcaat aaatgttcac tgatttcttt cacggtaata cccggggcac ttcttgtcct
      241 ctggtaacaa aggtaactgt acattcgtct ttgcaactga cagtgcatca cggttttgaa
      301 gtacctgctg ctgttgtttt tttactcaga aagcgcagtt actctttata ttttcccagc
      361 ctatctctta ttttaatata catccactgt ctggcgtttt aaggggctgg ctcactggcc
      421 aagttgacat taatcatcat tcctgtcttt gtcagcggag acttctgctg tcagtttttt
      481 catccataca gagtgacatt aattatccgt aagcgcaccg tgaaggacgt ggggtaaaaa
      541 ttagtttaca gatagagtga cgttccaggg caacaactct ttcacgcggt tggctggcca
      601 ggtgttgatg acactgatca cgtggcgtag ccacgtttcc ggctcgattc cgttaagttt
      661 gcagctaccg atcaggctgt acatcactgc cgcactctcg cctcctccat cagagccgaa
      721 gaacatgtag ttacgccgcc ccagtgcaac tacccggagg gcgttttcac acagattatt
      781 gtcgatctcc acccagccat tgcggcagta ttcgttcagg gcgtcccatt gcttcagcag
      841 ataggtgaac gctttcgccg tatccgagtg acgcgacagc gtgctcatct gcccctggag
      901 ccactcatac aacgactgca ttagcggtac cgttctggct tttctgaccg ccagtcgctc
      961 ttctgccgga ctgccgcgga tctcagactc gatagcgtac agtgcctcga tagggctgcg
     1021 tttgggaaac gtacaatatt gtacggttgc gctattgcgg tagggtgtac tccaacgcta
     1081 tacggtattt ggcagacctt tagggacatg agataaccta aatgcaataa gagaaaagca
     1141 ataacttaaa ggcgatagct gatcacgatg aatcaatcag ttacttatcc gcatcgatta
     1201 aaaaatgctc ctaacgtaca ataattccgt ttcccaaacc gacccctctc atagctcacg
     1261 ctgttggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgta tgcacgaacc
     1321 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1381 aagacacgac ttaacgccac tggcagcagc cattggtaac taaacaaggt ggattccgat
     1441 attagagtct tgaagtgatc acccaactac ggctacactg aaaggacagt attcggtatc
     1501 tgcgctccac caagccagtt accacagtcg attagaagag gtcgaagact tctaatcgga
     1561 cggaaaaagc cgccgcaagg cggctttttc cgtttacaag ccgaagatta cgcgcagctt
     1621 aaaaggatct caagaagatc attttaattt tgctgatcgt tgctccctac cagtgaccat
     1681 acggataggg cttttccttg ataaaatcgc ggataaatgg atcgtaacca ttagccttga
     1741 tgtaactgaa atcaatctgg ttgggaacgt ttttgcatat cagcatatca tctcccgtca
     1801 gagtgaactg ccgggcagga tgttggagta caacaggctc gccattatcc tccagccaga
     1861 tccgcacggc ccctcccggt gccaatccga taatcatatt gtcccgataa tacgtttttc
     1921 ccggcttata ggtatcagga taggcagtca acatctgctg ccacgtatcc cggctgaacc
     1981 agatctccgt ctcatatgcc tttttatcga taaccgaatc ccaacacacc gtcatcctga
     2041 ccggcagtgc cttgccaatg ttaaaactgg ctgaaaaact gcctacgcgc tggctccatg
     2101 tatccacact actctgactt ggtctggctg catcaagctg ccgaaatatg ctttcccgta
     2161 catcaccgtc ctccagatac accattgtca ccagtgctgg caggtttttc gggtaaaaaa
     2221 aattaaactg ccagtggtca taaggcagct tctgccccca ttgatccata ccgtcgccca
     2281 acagcgtctt cacgccagcc ctacggtcat ggcagccgac agccagcatt acgcccacta
     2341 tcacgcccac catcagcggt aatcgtctca ttcctgcctc ctcagaacag tccgtccagc
     2401 gcatccgcgt ttacccgcgc ccgctcttcc cactgctgct ggtcgtccat ccgccacacc
     2461 gtccgcaccc agcctttgcc cggtgccatc ggccacacga acgagcgttc gtcagtcgga
     2521 gcatagttat ttcgcagaaa aacttctccc gaggacggac tcacccacag gctcaagtct
     2581 gagtcgatgt tccagttcgc cgagcaatgc aggtaacgcc cgatggtccg catatcctcc
     2641 gccgtgaagc cctcaggctc cgcgccacgc cggacagcac gcccctgagc tatggctttg
     2701 tcacacaggg gaagcagttc tgacggcaac gccagctctt tgttactgct gagtatcgga
     2761 gtaaacacag ccccggcgtc ttgtgccgca tccagcatca cccgcaaact caccttctcc
     2821 cagtcgttcg gcacatccct gcgcgtcata aacactgccg cgcccacttt cttctcaaaa
     2881 atagacgcac gcgccttgtt ctgattgacc agtggccacg aaatcatttt cgtctccacc
     2941 tcaccgtgcg gcatcaggta cttcagcgcc ggcagtgagt acagcacctg acgcatcttc
     3001 tccgccttac ggtacacctc cgtctgcatt tcatccgcgc tgtcgcgcgt cacctcaaaa
     3061 tccggcatcg tcagcaggga gatttcatcc tctcctgcgt tatagccccc gccaatgtcc
     3121 gagtgcgccc ccggcagcgc cagctccggc cacatccccg cgatgctgtt caggctgaag
     3181 ttataccgac actcatgcat cgcgctaatc tgaaacacct tctgcgccac tgaaggacgc
     3241 agttcgagat tcaccccggg attgctgcgt ccgttaacgt cgtagaagtt caacaggctc
     3301 ccgatagccg ccactgtgtc aaacagccct aaaaagcgca cctctcccgc cagtttcccg
     3361 tggtgcccgg tcaggtctgt ccctttgtct attgccgccg ctatcgccgc atcctggttc
     3421 atcacccggt tggcaaaatg ccgtgcagcc gccgcgccgc ggctgaatcc gaacacgtca
     3481 aactgcacct tcgcaatcgc cactttgtca cgattaagtt tgataaagcg ggtcagttct
     3541 gcgctgatct tctccagtgc ttcatcggtc tttgtcacca ccccttcaaa ctgtcggata
     3601 aggctgctgc ccagcgcttt acccagtatc gaatcacttt ctccgtctgc cgtcccgatg
     3661 ccgctgatat aggctttgac ctgcgcctca gtctcgctgt cgttaagttc atcgctttgc
     3721 tgataaagat cgaacagccg ggaaatatta gtgtggccgc cctgccagct tgcattaccc
     3781 agcccagctc tggcgcgctt ttcatacttc tcacatgccc tagcccgctc ctcgtcaacc
     3841 agattggcac agtgggcata tgccagccgt agatcgtctg cgttcgcccg gttattcccc
     3901 gtcccgtcaa agaacacccc caccgtcagc gttatccccc tgaccggcac ccgccgacac
     3961 tggatggttt taaaactgtt gtgtatgctg gttcgatggg tactgtcccc aataatttca
     4021 taccggtaat aacaggtatc gcccggcaca tttttttcat cttcttcgac cataaaaatc
     4081 cttatttatc tgaaagataa aatcagtagc aaaatccttc gggacttttt tacatctgcc
     4141 tgttagacgt atttaccata atcccttaat tgtacgcacc gcagaaacgc gctcagcgcg
     4201 attctggcgc aaaaaccgca aaaatagatg ttttcccccg ggtaaaccgg aaaaatgcgt
     4261 caggattgtt ttcatggcac tgcatgacta tgcatgaaac tgaatggcga tcggtttggg
     4321 cgcgcctgat gcccataagg cgcattttcg gacgttttca gccctgatga gaagaaatca
     4381 gacagtagtt acagacgagt cgtgagcgat tcaccccggg agtcatcagc gagtcatcca
     4441 gtatttttcc tcgcgactct ctggcgactc gctttttctg aacaccagag agacagtgtg
     4501 ttgagtcatc gataaatcac cgacgactcg ttgccgaatc atccagtagt cgccgacaac
     4561 tcgcttttgt ataaatct
//
LOCUS       DAZUCE010000309         4538 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZUCE010000309 DAZUCE010000000
VERSION     DAZUCE010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.311"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            173..1312
                     /locus_tag="ACHY03_004769"
     CDS             173..1312
                     /locus_tag="ACHY03_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HFS3907900.1"
                     /translation="MNKIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            1432..2700
                     /locus_tag="ACHY03_004770"
     CDS             1432..2700
                     /locus_tag="ACHY03_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFS3907901.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEMISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLAGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            2708..3853
                     /locus_tag="ACHY03_004771"
     CDS             2708..3853
                     /locus_tag="ACHY03_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFS3907902.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            4321..4467
                     /locus_tag="ACHY03_004772"
     CDS             4321..4467
                     /locus_tag="ACHY03_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907903.1"
                     /translation="MAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYR
                     GMSM"
BASE COUNT         1076 a         1162 c         1199 g         1101 t
ORIGIN      
        1 aggaaaaaag aggatacaag ctttttgttg tatttttatt tgtataaacc gacaaaaaag
       61 aggccatact tcccgctttc tttgtctgtt tacacggaac atttcaccac tggatacgct
      121 aatagatcgg atatgagcac atattgcgcc cgcctttcat gcagagcata aaatgaacaa
      181 gattcacgct aattattacc tgcaagattc taccgcacgc cagatagttc aacgcacaat
      241 ggggattatt ccgtactcgg taaatgtgat gaacgagcat gggatgatca tcgcttccgg
      301 ggaagcctca cgtttacatc aacgccatga aggtgcggta ctcgcattga ccgaaaaccg
      361 ggtcgtcgaa attgattccg cgaccgccaa taagcttaaa ggcgttaagc ccggaatcaa
      421 tttaccgatt aattttcgcg accagctgat tggtgtactg ggcattaccg gcgagcctgg
      481 tgaagtgaag gcatatgcac agttggtgaa gatggcttcg gaattaatca ttgaacaaat
      541 ggctctcctc gagcaaaaac agtgggataa gcgttaccgc gaagagctga ttaatcagct
      601 gattttacgc gatccgccac cggactcact gaatgcggtg gtctcatggt tgggaattaa
      661 tctccagctt ccgcgggtgg tggtgattat ggaactgcaa caacctgatc gcgacgcact
      721 gcataatctg atggagtatt ttgaatatcg cgcccgcgac catttggtca cgttttccga
      781 ctttaatgat ctgataatct taaaacctat ctcgctaaaa aacggcgtct gggataaatc
      841 gttggaatta agcgatctgc agagacttaa acattacgcc cagtcctgtg gtttttcccg
      901 actgattgtc ggcggctatt tctctggaga aaacggcctg catcgctctt ttcagaccgc
      961 gaaatccacc cagtccatgg cgaaaagact gaagttgaag catcagttca tttttcatga
     1021 cgactatgcg ctggcatcat tattagatgg gctttctggt acctggcaag cggaagaatt
     1081 atccaaaatc tggcgcaatc tggttgccaa ggacaccaaa ggtatgctgc aaaaaacgct
     1141 gcagaattat tttgatcata attgtgatct ttcgcaaaca gcgactggct tgcatattca
     1201 tgtcaataca ttacgctatc gactgcagag agtggaagat attacaggaa tacaaatcaa
     1261 taacttaaag cagttattct ggctgtatat cggcatggag ttacaacgct gaactgtatt
     1321 tccctccaaa tttttcgccg tctgacgtcg tatttttagc cgatacacac aagtctaacg
     1381 cggctgcttt ctccatactg ccccctgtta ttttcatgga gaaagcagaa gatggtttca
     1441 ataacagcaa ctggcgccat catcgcactc gtggtggcga tcgttttaat tttacgcaaa
     1501 gtctcgccgg cttacggcat gattgtcggt gcgctggtag gcggtctggt cggcggcgcc
     1561 gatctggtgc aaaccgtgac gctaatggtc actggcgcgc aaggcattac caatgccgtg
     1621 ctgcgcattc tcgcagcagg tgtactggcc ggcgtgttga tagaatccgg cgcggcaaac
     1681 accatcgctg aaactgtggt gaaaaaagtg ggggaaagcc gggcgctttt agccttagct
     1741 attgcgacga tgattctgac cgccgtcgga gtatttatcg acgtggcggt tatcactgtt
     1801 gcgccgattg ccctgtctat tgcccgtcgt gccggtttgt cgaaactcgc cattctgctg
     1861 gcgatgattg gcggtggtaa agcgggtaat gttatgtcgc ctaaccccaa tgctatcgcc
     1921 gtttcggaca cctttcatgt tcccctcacc tccgtgatga tgaccgggat aatccccggt
     1981 ctgttcggcc tgatcgtggc ttatttcctg gcaaaatgtc tcagccagaa aggcctgatg
     2041 gtgatgcccg acgaaatgat ttcacacgac aaccacggca agcagccggg atttcttgcc
     2101 gctatcagcg cgccgctcgt cgccattgtg ctgctggcgc tacgacctgt tgcgggtatc
     2161 gtcatcgatc cgctgattgc cctgcccgtc ggtgggttag ccggtgcgat ccttatgggc
     2221 aggtttaagc aaagtaacgc ctttatgatg tccgggctta cgcgcatggc gccggtagcc
     2281 atcatgctgc tcggtaccgg tacgctcgcc gggattatcg ccaactcgga actgaaagat
     2341 gtgctcattc atggactgac ctcttccggt ctgccttctt atcttctggc ccctgtttcc
     2401 ggcgcgatca tgtccatggc aacagcctcc accacggcgg gaaccgctgt tgccgccggg
     2461 gtattcagcc agacgctgct ggggcttggc gtatcagccc ttgccggttc cgccatgatt
     2521 catgctggcg caaccgtact tgatcacctg cctcacggca gcttcttcca cgccacgggc
     2581 ggcagtgtcg gcatgctgat ccatgaacgg ttaaaactgc tgccttatga aacccttgtc
     2641 ggttttgtgg tcgccctgat ttccacactg atgtttggcg tcttcggact tgccgggtaa
     2701 agacacgatg aaaatagtca ttgcaccaga ttcatttaaa gaaagtttat cggccatgca
     2761 ggtggccacc gctattgagc agggatttcg tgaaatttat cccgaggccg agtatgtcaa
     2821 attgccgatg gctgacggcg gtgagggtac cgtcgaatca atggttgaag caaccgaagg
     2881 ccattatctg ttccagaatg tcaccggacc gctggggacg ccagtacagg cgcgctgggg
     2941 tatgctggga gatgggaaaa cggcgatcgt tgaaatggcc tccgcatccg gactacatca
     3001 tgttcctcca gaatgccgta accctatgct taccaccagt tatggcacag gcgaactgat
     3061 ccttgccgca ctggaccacg gcgttgagcg aattattctc gggattggcg gtagcgccac
     3121 caatgacggc ggcgcaggta tgctacaggc acttggtgtg gcgatgcttg atacaaacgg
     3181 gaaatcgctt cctttcggcg gcggcgcgct aagcaacctg gcgctcatcg atgcctcagg
     3241 ctgcgacgct cgactgaaaa acgtttctgt caccgtcgcc tgcgacgtta acaatccact
     3301 ctgtggcgca acaggagcgt cagccatctt cggcccgcaa aaaggagcaa caccagaaat
     3361 ggttgcctcc ttagaagctg cgctgaccaa cttcggtata cgacttcagg acgcaaccgg
     3421 acgaaacgtg atgacgacgc ctggcgcagg tgccgctggc ggcatgggcg cggcgctgtt
     3481 agggattctg aatgcggagt taaagccggg agtggagatt gttattgacg cgctgcatct
     3541 cgatagcctg gtcgcggatg ccagcctggt aatcaccggt gaaggtcgtc ttgatagcca
     3601 gagcatcagt ggtaaaacac cgattggtgt cgcacgctgc gctaagcggt atcagaaacc
     3661 ggtgatcgca ctggcgggcg gcttaacgac cgaccaccag atagtgcatg agcacgggct
     3721 ggacgcggtg ttctccgtgc tgacgcaaat cgccactctg ccggaagcgc tacaggatgc
     3781 ggaatacaac ctccgcgttt cggcacgcaa tattgccagc gtctggaaat tatcacagca
     3841 gcagatgcgc taatcttcag tcaggccggc aagcagtagc tggcctggat attctttctc
     3901 accaggccca tgctaaaaca gcctgcttca gtcatcccac gcaaggcact agcatggcaa
     3961 aaaaagaggg gagtagtgga tcccaaaaga cggttaagcg gttaaattga atggtcaata
     4021 agggggctgt ttgggaaacg aatctatggt cactcccgtt tttgcaacac cgattttgac
     4081 gataagtggg cttgcttgaa tctatccggc gtctgaatgg gattttattc ccgcgcctcg
     4141 atgagttccg cgcctgatga acctcaagaa aatatacggc ttcaatgagc ctttccgttt
     4201 cacaggttcc tcaacaggcc ggtgggccgt tcatatcatc aataccgtga tgctgatact
     4261 ccgtacccac cggaaccgca cctgatgcgg ttccggctta agggctgtca ggactcgctt
     4321 ttggctgcca tcaacaaacc ggaagagatg ccggattatc tcttccgtca actcggtttt
     4381 aatcagacct ggcatgagtg gaagcgggac gaacagcaca ggcaacaaca acgccggccc
     4441 ggacactacc ggggcatgag tatgtaatgt cagggacagc tatacgccat aaaaaaatga
     4501 aatagatggc ctctctataa agaggccatg atatttac
//
LOCUS       DAZUCE010000310         4502 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZUCE010000310 DAZUCE010000000
VERSION     DAZUCE010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.312"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            1..2984
                     /locus_tag="ACHY03_004773"
                     /pseudo
     CDS             1..2984
                     /locus_tag="ACHY03_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            3476..3840
                     /locus_tag="ACHY03_004774"
                     /pseudo
     CDS             3476..3840
                     /locus_tag="ACHY03_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3850..4041
                     /locus_tag="ACHY03_004775"
     CDS             3850..4041
                     /locus_tag="ACHY03_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907904.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFSAL
                     KKENVGEFALRGVCVPGGE"
     gene            4075..4368
                     /locus_tag="ACHY03_004776"
     CDS             4075..4368
                     /locus_tag="ACHY03_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFS3907905.1"
                     /translation="MAYHSPDVTLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMHEQMSRIIRKTGTDEILNKAPSCFP"
BASE COUNT         1264 a         1016 c         1117 g         1105 t
ORIGIN      
        1 tatcttcagt ttgctgatga ttatacagta acaacgagca atgacagttc atggtccgga
       61 ggaggcatta ttgtcaatta tggtacaact gtaaaatggg gtataaacgg ggtttccgga
      121 gatgaccttc ataaagttgg ggacggaacg cttattatta atggaaccgg gaaaaatgaa
      181 gggggactaa agattggggc tggtactgta atccttgaac aaaaagcgaa aaacaacgat
      241 tcaacggctt tttcttccat taacatcagt gggggaaact cgcgcgttaa actttcagga
      301 gataatcaga ttatacctga taatgtttcc tggggattcc ggggggggga tatttagata
      361 taaacggaaa agacactgag ttctcccggt tacaggctgt tgattatggg gctgcaatta
      421 ttaacagcag cacagacaag tcattattga cgttaaatct gtcaccattg aaaaaagatg
      481 aaatagctgt aagcgtgaag gcgttagata tgaacgccat ctttcagggg ggacacggga
      541 cagccggaga tttgtataaa acaacttttt acggtccgac acagtattat ctgctgaaaa
      601 agcccaaatt tggcagtgtg ctgatggggt cactgaaaaa caccagcgaa tggcaatttg
      661 caggaacaga cctgaatcag gccgttgata tggcaaagaa taataaactg acatcaagtg
      721 cacaggcttc atatctttat cacggtaagc tcctggggaa tatggatatt gtcattccgg
      781 agcttacagg caatgatatt ttaacgcttg atggctctgt cagtatatcc ggggatatgt
      841 caaaacagga cggtgctctt atcttccagg gacacccggt tattcatgca gggcaaactg
      901 tttctgcatc gcagagtgac tgggagaaca gggagttctc actcaacaat ctgaatctta
      961 ataatgcgga cttcagtctg tcccgtaatg catttatgaa cgggaatatc agggccgtta
     1021 accagagcac tgttattatc ggcggagata cagtctttac tgataaaaat gacggaacag
     1081 gtaatgatgt catcagtgtt gaagggaaat ctgctgccgc aggaacatcc tcctatacag
     1141 ggcatatcac tctggagcaa aaatcagcac tggatatccg cgataatttt cgtggcgggg
     1201 ttacgtctga agacagtcat atcaatgttt cttcatcttc agtcctgttc tcagatgcat
     1261 cgtcatttat aaacagctcc ctgaatattc ataaaggagg tgcgctgacc gctcagggag
     1321 ggctgtttac aagtggaagc attgatattg gtgacgcttc ccttctgctt accggtacac
     1381 cagtgaattc agatgatgct gcttttttac cgaccatcaa tatggctgat ggcggattta
     1441 aactgatgtc tgattcatca gtactgaaag ccagagacca ggcatctgtt gttggtgata
     1501 ttatttctga taaacaggcc acaatcagct tcggaactga atcaggtaaa gagggcatat
     1561 tgtctgagaa ggcatcccgg ggactcgcgg taggattact gagtggtttt aatacggcat
     1621 accgcggtgc aattcatgcc ccgtcagcat ctgccactat gaacaacacc tggtggcaac
     1681 tgacaggaga ctcctcactt cgctcgttaa aaaataccgg aagcatgcca tattttacag
     1741 gaagtgcagc gaataaagca ttccatacac tgacggttga tgagctgacg acgaatggca
     1801 ctgcgtatgc catgcgtacg gacctgaaaa atgcggataa gctggtagta aaccaaaagc
     1861 tgtcaggtaa ggacaatatt ctgctggttg attttctgaa caaacccacc ggagaaaaac
     1921 tggatattga actggtgagt gcaccgggga acagcagtaa ggatgttttt aaaggaagtg
     1981 aacaggcaat aggtttcagc aatgtcacac ctgttattac agctaaagac gccggagata
     2041 aaacaacatg gaacctgacc gggtacagga tggcagaaaa tcccgccgca acccaaagtg
     2101 cctcaggcct tgcatctgtg gggtacaaat catttttgag tgaggtcaac aacctgaata
     2161 aacgtatggg tgacctgcgt gacatcaatg gtgaagctgg cgcatgggca cgtatcatga
     2221 gcggaaccgg ctctgccggt ggtggtttca gtgacaacca cacacatgtt caggtcggtg
     2281 tcgacaaaaa acatgagctg gacggactgg atttgtttac cggcttcact gtcacacaca
     2341 ctgacagcag tgcctctgct gatgctttca aaggtaaaac aaaatctgtg ggggccggac
     2401 tctatgcttc cgccatgttt gattccggtg cctatatcga cctgattggt aagtatgttc
     2461 atcatgataa tgagtacacc gcaacctttg ccggactcgg aatccgtgat tacagtacgc
     2521 attcatggta tgccggtgct gaagcaggct accgctgtca tgtcactgag gatacctgga
     2581 ttgagccaca ggcagaactg gtttacggtg ctgtatccgg taaacagttt gcatggaagg
     2641 accaggggat gcatctgtcc atgaaggaca gggactacaa tccgctgatt ggtcgtaccg
     2701 gtgtggatgt gggtaaatcc ttctcaggta aggactggaa agtgacagcc cgtgccggtc
     2761 tgggctacca gttcgacctg ctggctaacg gcgaaaccgt attgcgggac gcatcaggtg
     2821 aaaaacgtat caaaggtgaa aaagacagcc gtatgctgat gtccgttggc ctgaatgcag
     2881 aaatcaggga caacgtccgc tttggactgg agtttgagaa atccgccttt ggtaagtaca
     2941 acgttgataa tgctgtcaac gctaacttcc gttactcgtt ctgatactcc gtattcaaaa
     3001 gggcctgcgg gccctttatg ctcaggacct gccgcccagg tatacatttc cggggtatca
     3061 gtcgatttgt ttacttaacc gccatgacca aagccggcag caatgtcggc tttttacgtc
     3121 catatattcc actcatccgg aactgtgaca cagaacctga atacaggatt gatcaccggt
     3181 gcccacaaat ggcacatttt cagtaagatt ctgaatgtta cattcccccg tcatgtgtgg
     3241 cacaactgtc accgggaaag aaacggaaag cacatgaaaa acatcaaaac tcacaccgga
     3301 ctccttatcc acaaagaaca agcgcggcgc gttcgtcttc atgaaactcc ggccgcctgg
     3361 tgccacacgc accgggaatg ctacagcaaa acgaccggtc gccgctgcgg cagccctgac
     3421 agtctcagca ggctgattct gagcagcatt cagcccatag aatgagatga cccgtatgaa
     3481 tgatggcaga cagacgcctc cgtcccctga ggcatggaaa atccggtttc tggcgaacgg
     3541 tacacccggc acatggtttt acaccgatga cgcctccgtc acaaaagagt gggggcggga
     3601 atattgtgac aatgtggaag tgttccctct gtgctatatg catgatggtc ccgttttccg
     3661 ggggcgctat attgatgagc tcagcgccgc acggaacaga atgctccata tgcaccacat
     3721 actgccggaa tgagcccggt acagagaaaa ttttcatgac gcagaatgta ttcgtgaaat
     3781 tcacgaagcc ccctgcaggc tcccggcatt ctgcaaaaat acaaacgcac aaaatcataa
     3841 ggcagttcta tgtggatact gattttctgg ctctcatccc cctttaatgt ctcctccggc
     3901 gatgcccggc aacccacact ggccgttcac agtcaggagt tccgtactga aaaagcctgt
     3961 cgggatgcct tcagcgcgct gaaaaaagaa aacgtgggag aattcgctct ccggggcgtc
     4021 tgcgtccccg gcggagaata aaccggagat gatgattctc cacaggagta ttcaatggca
     4081 taccattccc cggatgtcac actacattat ccgtatctgc gccggatgtc ctgggcgcag
     4141 ataacggcag cagctgaaga tgcggaaata catcatgatt atgcccgggc attaatattg
     4201 tggcagcatg catatcatgc tgccacgctg accctcaaca aaaacctggc agttgctaaa
     4261 atcgacttct gtattaaacg tatccggatg catgaacaaa tgagcaggat aatccggaag
     4321 actggcacag acgaaatact caacaaagcc ccttcatgct ttccttgaga acaataattt
     4381 tatctttatg tgaatctcat tttttataat gatgtcagat gattgaaatg ctaaattata
     4441 tctgaatcaa cttttccttt gctaaaggta tcgaaataac tgcaaggctg tattttgtaa
     4501 tt
//
LOCUS       DAZUCE010000311         4435 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZUCE010000311 DAZUCE010000000
VERSION     DAZUCE010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.313"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            71..811
                     /locus_tag="ACHY03_004777"
     CDS             71..811
                     /locus_tag="ACHY03_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFS3907906.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPTDNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(941..2911)
                     /locus_tag="ACHY03_004778"
     CDS             complement(941..2911)
                     /locus_tag="ACHY03_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFS3907907.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLG
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIVVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(2922..4322)
                     /locus_tag="ACHY03_004779"
     CDS             complement(2922..4322)
                     /locus_tag="ACHY03_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3907908.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNHP
                     MMSRIATDLRQRHVQP"
BASE COUNT         1065 a         1149 c         1192 g         1029 t
ORIGIN      
        1 tgcaccggag aaattgtaag cattactatg ccgactcttt tttatcttat tttaaaatgg
       61 aataccggac atgttaatta accgcaatat tgtggcgtta tttgcgttgc cttttatggc
      121 aagcgcaact gcttctgaat tatccattgg tgctggtgcg gcttataatg aatcgcctta
      181 tcgcggttat aatgaaaata caaaggcaat tccgctgatt agctatgaag gtgatacttt
      241 ttatgttcgt cagaccacgt taggttttat tctgtcgcaa agtgaaaaaa atgaacttag
      301 cctgaccgca tcctggatgc cgctggaatt tgaccctacc gataatgacg attatgccat
      361 gcaacagctt gataagcgtg atagtacggc tatggcgggg gttgcctggt atcaccacga
      421 gcgttgggga accgtgaaag cctctgcagc tgcggacgtt ctggataaca gcaacggctg
      481 ggtgggggag ctatcggtat tccacaaaat gcagataggt cgtctgtcgc tgacacctgc
      541 gctgggcgtt ctctattatg acgagaattt cagtgactat tactatggca tttcagagag
      601 tgagtcccgt cgtagcggtc tggcaagtta ttccgcgcag gatgcctggg tgccctatgt
      661 cagcctgacg gcaaaatacc cgataggaga gcacgtcgta ttgatggcga gcgcaggata
      721 cagcgagctg ccggaagaga ttaccgacag cccgatgatt gatcgtaatg agagttttac
      781 ctttgtcacc ggcgtgagct ggcgttttta attcaccggt ggatatcggt gcggctggcc
      841 tacgctgtcg caccgacttg tatgcctgat aagacgtgtt aagcgtcgca tcaggcaatg
      901 tgcttcttac gttggatgcg gcgcgagtgc cttatccaac ctacggatgg caatgctttt
      961 cctcattcac ccagatccgc atttcgcctt caccccggtt ggcccagcta aaccacggga
     1021 taaaggtcag agtttgcgtc tggcgtttgg ctggcgcgct gtcgtaatgc cacagcggtt
     1081 gctgctctgg attgctctgt tcataccggt aacccggtgc ctggattaag atcttatggc
     1141 taaacaatcc cttgccttca aatgtcgtaa atggcgcatc ggtgggcagc cacagattat
     1201 gcagtgactc gccgttgtcg gcctgttcca ggcaatacac cagcgggccg cgctgaatcg
     1261 ccacttttcc ggcgacgtga cgcaccagcg ggttaccgta aacgcggcgt accggcatcg
     1321 gcaaagtcag attcagcgta tcgccctcct gccattcgcg ggtaatgtgc aaataccctt
     1381 tacgaatatc ctgctcgacc tcttccccat tcaatatgat ctgcggctgt gtgcaccagt
     1441 ccggcagacg taaagccagc gtatgacgta ccggctgggg cgattcgacc acaatcgtca
     1501 cctgctcctg ccacggatag ttcccgctaa cccgcaggcg cagcgtgcca ttttctaccg
     1561 gcacttccat gctgtttcct gcgtagatgt tgatatacaa cgcatcttca cgcggcgtgt
     1621 agagataatg accaatcgag gtcagcacgc gggcgatatt tggcggacaa caagcgcagc
     1681 caaaccaacg ctggcggatc ggtttaacgt gatcgtagat atggttgaat ttcagcgatt
     1741 ttggatgtac ttccagcgga ttcacataga agaaatgttt gccatccagc gccatgccgc
     1801 cgagcacggt gttgtacagt gcgcgctcca tcacatcggc atattgactg tcgccttcca
     1861 tttccagcat tcgtcgggca aacatcatca ggccgatgga ggcgcaactt tcagcgtata
     1921 ccgtgtcatt cggcagatcg taatcgctag tgaacgcttc gccgctgctt tgcgagccaa
     1981 tgccgccggt aatatataac tgacgctggg ccatattgtt ccacagccgc aggcagtcct
     2041 gacgcttgct gtcatcgtga cttaaacgcg cgagatgcgc gacaccggtc atcagataga
     2101 caaaacgtac cgcgtgaccg atggcggttt gctgttgcgc aaggggcaaa tgtgcctggc
     2161 tgtaggcttt atctttcacc atccacgccg ggccgtaggt gtgccagtgc gatgtctgcc
     2221 cgcgcttttc atattcctgg tcgtaatagt gcggttgcgc accacgctgt tcgacaaaat
     2281 agttcgtcag cgccaggtag cgcggctctt cagtcacttc atacaggcgc attagtgcca
     2341 gttcaatttc cgggtgacca ggataaccgt gtaacttact ttcatctgga ccaaatacgc
     2401 tgtcgatatg atcggccagg cggcaaacga cccccagcaa gcgccgtttt cccgtggcct
     2461 ggaagaaggc gactccggct tcaatcagat gacctgcgca gtaaagttca tgacactccg
     2521 ccagattgct ccagcgttct tcgggtgctt ttaccgtaaa gtaagtattg agatagccgt
     2581 cttcgcattg ggcggaggcg atcagttcga ttacctcgtc ggcggttttt tccagttcgg
     2641 cgtccggctt ctggcacagc gaccaggcta ccgcttccag ccatttggcg acgtcgctgt
     2701 cctgaaacac catcccgtaa aattcaccct cctgaagtcc ggcggcaatg cgaaagtttt
     2761 caatcgcatg gctgggttcc gcttctggga tacgatcgtt caaggcatcc cactgataag
     2821 gaatcaccac gtcgcggacc agttgttggt actgaccgag gaacggatcg ctgaccgtca
     2881 gtttatgcag atcgacttcc gaaatgttca tcttgtgctc cttaaggctg tacatgacgc
     2941 tgacgcaggt cggtagctat gcgtgacatc ataggatgat tgagtttgca aaagcggatc
     3001 gcccctgcca gcagcaggtg gaatagcgca ggcagtaatg tttccatcgc cgcgatcccg
     3061 ccaagcgaac tggcggtctg gttttccacg ccaggctggt aggcgacaaa aataaacagc
     3121 aggctgatga tcccggcgct ggaggcccag gccagcttga tgaaaaacag attgaaggcg
     3181 aagttcatgc cggaagaacg tacgcgggtt ttccactcgc cgtagtcatc ggcaaaggcc
     3241 atcaatgaga agtgcagtgg caatgtgaag ccaagaatta cgccattacc gaggatcacc
     3301 gccagccaca gcgtttgata agccgggccg gagggcagga accacatcaa caccgccagc
     3361 gcagcgagga gcaggttggt gtagtagtaa attttgacgg tatcgaaacg ccgcgttaac
     3421 gggctgacaa tcaccgagcc gataatggaa gcgaaggtga ccatggtgaa gaacagcgac
     3481 gtgtagcccg tgctgccttg taagacgtag gtaatgaagt acatataccc accgccgcga
     3541 atattaaaga cgttaatcag caggaaagac atgaccagca tcagcagcag ttggtcgttg
     3601 ttccgcagcc cggcaagatg ttcgcgcagg gtaaatttcc ccattgtgga gagtggcacc
     3661 cgttcacgaa cccagaaaaa acagcacagg aacatcacca cggcaatggc gcacagcacg
     3721 ccgacgccca attgatagcc gcgcgcggca ttaccctgac cgaagagatc taccatccac
     3781 ggtaagccta ctgaaaccag aaatcccgct acaccacaga gaacgaatcg ccaggactgg
     3841 caggagatca cttcattgtg gcgggtggtc atggtgttga tcagcgcgca gtacggtacg
     3901 ttgatggcgg tataaccgac tgaaaggagc aggtaagtac cgaacgccca ggcgattttt
     3961 actcccatgc tggcatctgg cacggtgaac gtcaggatgc cgataatgcc gatgggaatg
     4021 gcaatccata gctgccacgg acgaaagcgc ccccatcggc tttgcgtccg gtcggcaatg
     4081 acgcccatca ccgggtcgga gatagcatca aacacgcgca gtgcaataaa cagcgtgcca
     4141 accagcgccg gagttaatcc aaaaacgtcg gtgtagaaaa aggtgagaaa gttcatgatc
     4201 aggcaagtta ttacggtgcc gcctgcgtca cccaggccgt agcctatttt ttcccgtaca
     4261 gataacgcat cgtcaatttt acctttagcg atatcagcgg tagtaatcgg tgtagaggtc
     4321 atagatattc ctcgtgaata gggagcaatt tttagtttta ttgaaatgac ctgattaatt
     4381 ctgccgtgaa cgggaatatc aacaaggcga gaaaagagta aaaaaaggtg atgat
//
LOCUS       DAZUCE010000312         4387 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZUCE010000312 DAZUCE010000000
VERSION     DAZUCE010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.314"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..207
                     /locus_tag="ACHY03_004780"
     CDS             <1..207
                     /locus_tag="ACHY03_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS3907909.1"
                     /translation="LTLIPSGVNFDADENDPVKLLIVLAATDSTSHIEAISQLAKLFD
                     NEKDIQAILTAKTTQDILSVIARY"
     gene            223..510
                     /locus_tag="ACHY03_004781"
     CDS             223..510
                     /locus_tag="ACHY03_004781"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS3907910.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            523..1779
                     /locus_tag="ACHY03_004782"
     CDS             523..1779
                     /locus_tag="ACHY03_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFS3907911.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLSDFANIFQYAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     NIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(1995..2279)
                     /locus_tag="ACHY03_004783"
     CDS             complement(1995..2279)
                     /locus_tag="ACHY03_004783"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3907912.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQVVKLALQPRVSVARIAREHDIND
                     NLLFKWLRLWLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(2410..>4387)
                     /locus_tag="ACHY03_004784"
     CDS             complement(2410..>4387)
                     /locus_tag="ACHY03_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFS3907913.1"
                     /translation="VYEKMDKQRLGYVVARLGSTENRQQFFSTSISRSEEVLPDSPSA
                     NMIDDVLQQVKKHLNAINQVASLKAEINELSVEYKYLQQWQSQNLRPEELFSHKYRLS
                     SRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLSYYE
                     KSIREKQISLNEYEKVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAETYRD
                     EFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNVIVV
                     GDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCHPKI
                     IQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDEESS
                     RGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQQSRNIAF
                     VDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIRYYADDGEIFESPVISA
                     FDLLYSEYDKSLERLNSRLNSDDSRFKSEQIVACLLRDILSQDSYRSMMFHSQIALNQ
                     LVLLERGDFTHRELLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERDELK
                     NSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLSG"
BASE COUNT         1157 a          986 c          959 g         1285 t
ORIGIN      
        1 ctgaccctga tcccttcagg tgtgaatttt gatgccgatg aaaacgatcc agtgaaatta
       61 ctgattgtgc tggctgcaac cgacagtacc agccacattg aagcgatttc tcaactcgcc
      121 aagttattcg ataacgaaaa agacatacag gctattttga cggccaaaac gacgcaggac
      181 attttgtctg ttattgcgcg ctattaatcc tgaggaacat ttatgaaaat tacagtggtt
      241 tgcggtaacg gcttaggcac cagcctgatg atggaaatga gcattaaaag cattttgaag
      301 gatttgtccg tcagcgctga tgttgatcat gtcgacctgg gctccgcgaa agggacgccg
      361 agtgatatct atgtcggcac caaagacatt gccgagcagt tagtcgcgca gtctgtcgca
      421 ggcaaaatcg tcgcactgga aaatatgatt gataagaaag ccatgagaga acgtttatcc
      481 gtggcattaa ccgaattagg cgcactgtaa gctgggggcg atatggattt ctttcgtttt
      541 ttgatgagcg atgtactttc cgaacccgca gtgctggtcg gtttaatcgc cctgattggc
      601 ctgattgcac agaaaaaacc ggtaaccgaa tgcattaaag gtaccgtcaa gaccattatg
      661 gggtttgtga ttttaggcgc aggggcgggt ttagttgtgt cgtcactgag tgacttcgca
      721 aacattttcc agtatgcctt tggtattcag ggtgtcgtgc cgaacaatga agccattgtt
      781 tccgtagcgc agaaaagctt cgggaaagaa atggcgatga ttatgttctt tgcgatggtt
      841 atcaacatta tgattgcccg tttcacaccg tggaaattta tcttcctgac cggtcatcac
      901 acgttgttta tgtcgatgat ggtagcggta attctggcaa cagcaggcat gaccggcatt
      961 acgcttatag ccgttggctc tttggttgtt ggcgtggcaa tggtcttttt cccggctatt
     1021 gcgcatccgt acatgaagaa agtgaccggt tctgatgacg ttgccatcgg ccacttctca
     1081 acattatctt atgtattggc cggtttcatc ggcagcaagt ttggtaataa agagcactcg
     1141 actgaagaca tgaatgtgcc gaaaagtctg ctgttcttgc gcgacacgcc ggttgctatc
     1201 tcttttacca tgagcataat tttcctggtg acgtgcctgt ttgcgggtgc agatgcggtg
     1261 aaagagctta gcggtggtaa aaactggttc atgttctcta tcatgcaatc cattaccttc
     1321 gcagctggcg tgtacatcat tctgcagggt gtgcgcatgg tgattgcgga gattgtcccg
     1381 gcattcaaag gtatttcaga caagctggtt ccgaatgcca gacctgctct cgattgcccg
     1441 gtcgtcttcc cttacgcacc taacgctgta ctggttggtt tcctgagtag ctttgctgca
     1501 ggtttaatcg gtatgttcac gttgtatctg ctgaacatga tcgtgattat tcctggtgtg
     1561 gtgccgcact tcttcgtagg tgcagctgca ggggtattcg gtaatgcaac gggtggacgt
     1621 cgtggtgcga ttttgggtgc cttcgctcag ggcttgctta ttaccttcct gccagtattc
     1681 ttattgcctg tgctgggtaa cattggtttt gctaacacca ccttcagtga tgctgacttt
     1741 ggcgcactgg gtattttgtt agggattatc gttcgctaat attttgatga cattaatatc
     1801 ctccaggatt tcaggaacgt ggaggatatt aattcctctt attttctcca tttttctttc
     1861 ttccagtcag gactgcatct gattaatcgt aagcgctccg ggaagtacgt cgcgtaagga
     1921 ttattttaca gctgagaagt ttcagtgcag cagttcatgc acacggttcg acggctagtc
     1981 attgatcttc ataattacct cgcgtaaaaa acggggtaag gccgctcatc cagcgtcatc
     2041 tccacaggta ataattcaac gccaatgcca gaagagttcc ttaccggaag acgccgtgat
     2101 atacgccctt tgatcagcca gagcctgagc catttgaaca gaagattatc attgatatca
     2161 tgctcccggg caatacgggc aacagaaacc cttggttgta aagccagttt aaccacttga
     2221 agtttaaact catttgaaaa tgacctgcgg ggttcagctg ataatgtttt ttgttccata
     2281 acaggcatcc actagtttga aaaggtgggc acctacgtta ccaatactgg cttaatggct
     2341 acatacgacg gtcggtttac gcttaccaga aaactaatag tgctatatgc aatttttaat
     2401 attcagactt tagcctgaca ggcctgacaa aaacgcgccc aacttccctt ctatatcact
     2461 gtctatagta cgtaatctta gcaatggcag accacatttc ttcagtatac tgtttttaag
     2521 ttcatcacgt tcagcctgga cactggtcag atgatagcct ccatccactt caataacccc
     2581 taatggagtc ttcccgacct tatagtaaac aacaaagtca caactggccc gattacgcat
     2641 gaataacagt tcacggtgag taaaatctcc ccgctccagc aacaccaact gattcagcgc
     2701 tatttgtgaa tgaaacatca tgctccgata actatcctgc gacaggatat cccgcaacag
     2761 gcaggccaca atctgttctg acttaaagcg tgaatcatct gaattcaatc tactattcag
     2821 tcgttccagt gatttgtcat actcactgta cagcaaatca aatgctgata tcaccgggct
     2881 ctcgaaaatc tctccgtcat cagcgtagta tctgatgtaa cgaatcagtg ccgcaatatg
     2941 tccagcgtgg cgttcaaata catcattacc agtcaccaac gtaaatttgt ttcgggccct
     3001 tgaaacagcg acattcacca gttcaggatt atcaacaaac gcgatattcc gactttgctg
     3061 gctgctgcgt ttcttatcca gcacagtgga aaacacaatt tcatcgcact cccgtccctg
     3121 aaatttatgc accgtcgatt tcacaaaatc agcgggcagc actgtttcag ccagattaac
     3181 ctgtgcatta tatggggcga tatatcccct gctactttcc tcatcccagt aatgtccttc
     3241 cagagattca agctccctga gatttgaaaa attacgggta tggtttcctt ttgccgtgat
     3301 gatcagagat aatgaggctt cacctgaatc aagcgttagc ggtatcagag cattatcata
     3361 aaactgctta ttacaaaatt gaatgatttt aggatgacag cgataatgct ctttaagaag
     3421 agttactggc accatattcc tgaacaacat acaaaccgaa tcaagtagac tatatttctc
     3481 acaattatag tattctgccg gtggagatgg gctgttcggg agtaatacag gaacatgtgg
     3541 aagttgcttt cgatccccta caacgattac attacgggca catcctagtc ccagaatgcc
     3601 cggaacaata tcctgctgtg aggcttcatc aataatcaca taatcaagaa gagccccctt
     3661 accgatagag ttaataatag aatgcgtgct gctaccaata accggaaaac gctttataaa
     3721 acggttaaat tcatccctgt atgtctctgc cgagaatgaa tttcttgttg aaatatttct
     3781 tcgcagatgt tgttttaaat aaagcacaga ccatgatgtc aggcgctcca gtaaaatttt
     3841 aaaatcagat tcctttaata ctttctcata ttcattcaaa gaaatttgtt tttcccggat
     3901 ggatttttca taataagaaa gctgaagaga agtaaatagt gccagccttc tctccggaat
     3961 caccaatggt ttgactttca gtatcctgaa gtttaacaac aaatcaattc tgtttctgaa
     4021 tccaatccgc ctgtcagaaa ggtaatgaat ataagccatc aggtcagttg tttttcgtga
     4081 ggaaagtcgg tatttgtgag aaaaaagctc ctccggtcga agattctgac tctgccattg
     4141 ctgcaggtat ttatactcaa cactcagttc attaatttct gcttttaacg aagcaacctg
     4201 gttgatagca ttcaggtgtt ttttcacttg ttgaagtaca tcatcaatca tatttgcaga
     4261 cggtgagtca ggtaagactt cctcagaacg cgaaatcgac gttgaaaaaa actgttgtct
     4321 gttctccgta cttcccagtc gtgccaccac atatcccagt cgttgtttgt ccattttttc
     4381 ataaaca
//
LOCUS       DAZUCE010000313         4324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZUCE010000313 DAZUCE010000000
VERSION     DAZUCE010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.315"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1069
                     /gene="sufD"
                     /locus_tag="ACHY03_004785"
     CDS             <1..1069
                     /gene="sufD"
                     /locus_tag="ACHY03_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFS3907914.1"
                     /translation="ISPQQRDALALTLDAVRLVFVDGRYVPALSDATEGSGYEVSIND
                     DRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQGVAGEEVNTAH
                     YRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKLAFENPLSHHF
                     AHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSMLRINSLAMPVKNEVCDTRT
                     WLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNNLLMGKLAEVD
                     TKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYAFAAELTEALR
                     DEGLKQQVLARIGQRLPGGAR"
     gene            1066..2286
                     /gene="sufS"
                     /locus_tag="ACHY03_004786"
     CDS             1066..2286
                     /gene="sufS"
                     /locus_tag="ACHY03_004786"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFS3907915.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLSAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            2299..2715
                     /gene="sufE"
                     /locus_tag="ACHY03_004787"
     CDS             2299..2715
                     /gene="sufE"
                     /locus_tag="ACHY03_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFS3907916.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIKLQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            2864..3868
                     /gene="ldtE"
                     /locus_tag="ACHY03_004788"
     CDS             2864..3868
                     /gene="ldtE"
                     /locus_tag="ACHY03_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFS3907917.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYILPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVNSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(3932..4168)
                     /gene="lpp"
                     /locus_tag="ACHY03_004789"
     CDS             complement(3932..4168)
                     /gene="lpp"
                     /locus_tag="ACHY03_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFS3907918.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         1071 a         1057 c         1187 g         1009 t
ORIGIN      
        1 gatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc ggctggtgtt
       61 tgtcgatgga cgttacgtgc ctgcgctgag cgatgcaact gaaggcagcg gatatgaagt
      121 gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag tgtttctgca
      181 tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg gtcaacggcc
      241 ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag aggtgaacac
      301 tgcccattac cgacatcatc tggatctggc ggaaggtgcc gaagcaacgg tgatcgaaca
      361 ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta tcaacgtcgc
      421 agcgaatgcc cacttgcagc atatcaagct ggcgtttgaa aacccgctca gtcaccactt
      481 tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccaca gtttcctgct
      541 gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa acagcatgtt
      601 gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc gtacctggct
      661 ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg tcagcgacaa
      721 aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca aaacggatgg
      781 tcagatgacc aacaataatc tgctgatggg caaactggca gaagtggata cgaaaccgca
      841 gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg ggcgtattga
      901 tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg cccagcagat
      961 gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg ggcttaaaca
     1021 gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac tttttccgtc
     1081 gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt gccgctggct
     1141 tatctcgaca gcgccgccag tgcgcagaaa ccgagccagg tgattgacgc cgaggccgag
     1201 ttttatcgtc atggctacgc ggcggtgcat cgtggtattc ataccttaag cgcccaggcg
     1261 accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc ccgttcggcg
     1321 gaagagctgg tgttcgtccg cggcacgacg gaagggatca atctggtcgc caatagctgg
     1381 ggtaacagca acgtgcgggc gggcgataac atcatcataa gtcagatgga gcaccacgct
     1441 aacattgttc cctggcagat gctttgcgca cgcgttggcg cagagctgcg tgtgatcccg
     1501 ctcaatcccg acggtacgtt gcaactggag acgctgccta cgctgtttga tgagaaaact
     1561 cgcctgctgg caattactca tgtctccaac gtgctgggaa cagaaaatcc gctggcggaa
     1621 atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc tcaggcggtg
     1681 atgcatcatc cggtggatgt tcaggcactg gattgcgatt tttacgtgtt ttccgggcat
     1741 aaactgtatg gccccaccgg gattggcatt ctttatgtga aagaagcctt gttgcaggag
     1801 atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag tgaaggcact
     1861 acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg gggcatcatt
     1921 ggtcttagcg cggcgctgga gtatgtttcg gcgctggggc ttaataacat agccgagtat
     1981 gaacagaatc tgatgcatta cgcgctatca cagctggaat ctgtaccgga tctcactctc
     2041 tatggcccac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca ccacgcctat
     2101 gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca tcactgcgct
     2161 atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct ggccatgtat
     2221 aacacccatg aagaagtgga tcgtctggtg accggattgc aacgtattca ccgtctgctg
     2281 ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct gcgtaatttt
     2341 ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg ccagcgtctg
     2401 ccagaattac gcgacgaaga ccgaagccca caaaatagca ttcagggctg ccagagtcag
     2461 gtgtggattg tcatgcgcca gaatgcgcag ggaattatta aattgcaggg cgacagcgat
     2521 gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca gatgacgccg
     2581 caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct cacccaacat
     2641 ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg cgccaaagcc
     2701 gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat tgccggatgg
     2761 tcaatgttta cactgacctg cacaggagtg tttcggtttc agtcgaacat aagcgccatc
     2821 cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac gcgcgtcttt
     2881 gcttacactc acgcttatcg gcgcttttag tgccatccag gctgcctggg cggttgatta
     2941 tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata cggtgcagga
     3001 aggggataaa aaccttcagg ccatcgcccg acgttttgat actgcggcaa tgttgatcct
     3061 tgaagccaat aacactatcg ccccggtgcc aaaacccggt acgacgataa ctattccttc
     3121 gcaactgtta ttacctgatg cgccgcgtca ggggattatc gttaaccttg cagagctgcg
     3181 cctttattat tatccgccgg gagaaaatat cgtgcaggtt tatccgatag gtattggctt
     3241 gcaggggctg gaaacgccag tgatggaaac gcgtgtgggg cagaaaatcc ctaacccaac
     3301 ctggacgcct acggcaggca ttcgtcaacg ttcgctggag cgtggcatta aattaccgcc
     3361 agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc tcgcgcatgg
     3421 taatggcgaa tacctcattc atggcaccag tgcgccggac agcgtcggtt tgcgcgtcag
     3481 ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca gcgtgcggac
     3541 gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggagc ctaacgggat
     3601 gcgttatgtt gaagtacatc gaccactatc ggcagaagaa cagcagaacg ttcagacaat
     3661 gccatacata ctgccagcag gctttacgca atttaaagac aataaggctg tagatcagaa
     3721 gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gttgcggtga acagtggagc
     3781 aactcctaca gccagtaatg cgccttcagt agagtcagcg cagaatggtg aaccagagca
     3841 agggaatatg ttacgcgcga cgcagtaatg gtaaatagca gacaaaaaaa atggcgcaca
     3901 atgtgcgcca tttttcactt cacaggtact attacttgcg gtatttagta gccatgttgt
     3961 ccagacgctg gttagcacga gctgcgtcat ctttagcagc ctgaacgtcg gaacgcattg
     4021 cgttcacgtc gttgctcagc tggtcaactt tagcgttcag agtctgaacg tcagaagaca
     4081 gctgatcgat tttagcgttg ctggagcaac ctgccagcag agtagaaccc aggattaccg
     4141 cgcccagtac cagtttagta gctttcatta ttaataccct ctagattgag ttaatctcca
     4201 tgtagcgtta caagtattac acaaagtttt ttatgttgag aatatttttt tgatgggaag
     4261 gcacttattt ttgatcgttc gctcaaagaa gcatcgaacg ctgggtttta gataaaaaaa
     4321 ttga
//
LOCUS       DAZUCE010000314         4313 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZUCE010000314 DAZUCE010000000
VERSION     DAZUCE010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.316"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            78..239
                     /gene="rhuM"
                     /locus_tag="ACHY03_004790"
                     /pseudo
     CDS             78..239
                     /gene="rhuM"
                     /locus_tag="ACHY03_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            637..1821
                     /gene="setC"
                     /locus_tag="ACHY03_004791"
     CDS             637..1821
                     /gene="setC"
                     /locus_tag="ACHY03_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFS3907919.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPIEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1932..2855
                     /gene="yicL"
                     /locus_tag="ACHY03_004792"
     CDS             1932..2855
                     /gene="yicL"
                     /locus_tag="ACHY03_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFS3907920.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMVQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(2859..3677)
                     /gene="nlpA"
                     /locus_tag="ACHY03_004793"
     CDS             complement(2859..3677)
                     /gene="nlpA"
                     /locus_tag="ACHY03_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFS3907921.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITENPRHLRIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            3886..4179
                     /gene="yicS"
                     /locus_tag="ACHY03_004794"
     CDS             3886..4179
                     /gene="yicS"
                     /locus_tag="ACHY03_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFS3907922.1"
                     /translation="MKLTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
BASE COUNT         1066 a         1022 c          928 g         1297 t
ORIGIN      
        1 aatgcctctt catacgttct ccaacccctt gtaatactgt caatccagag attttctact
       61 gctcaatatt caatcggctt caggaatacc tgatcaaagg ttttgtgatg gacgatgagc
      121 gactgaagaa tccgcctgtc ggttcttctg tcctcgaacg catccgcgat attcgcgcca
      181 gcatgcgccg tgtttatttg caggtgattt gctcccctgc tgactctcag ccaactgtat
      241 cgccattaag gtgaagatgt tgttttacac gctccagaac accctttttt atacgactat
      301 tctattcctt taacagctgg cgaaaaaaac tgacctataa acctcaccaa taatcagact
      361 gccatcaaga ttaatttgtt aatttaatat atcaaatcaa cttgttttat ttttactatc
      421 aaaaatagct cattgcctta ctataattaa tatataaatt cggtgattaa ttcttaacat
      481 gcttttgcta ataatctcaa ttgctggccc tataatattg cgctagcatt gctttctggt
      541 tgtatcagcg ataccccaaa attcttaata caatactcat tcgactggta cttatttgta
      601 actcagttat attttgtcgc ccggtgattc agaagaatgc aaaaaacggc taccactcca
      661 tcaaaaatac ttgatctcac tgccgcggca tttttacttg tcgcctttct gacgggtatt
      721 gcgggcgctc ttcagactcc taccctaagt atattcctcg cagatgaact gaaagcccgt
      781 cctataatgg taggtttttt cttcaccggt agcgctatta tgggaattct ggtcagtcaa
      841 tttctggcaa ggcactccga taaacaaggc gaccgtaaat tactgattct gctatgttgc
      901 ttatttggag tgctggcctg cacgcttttt gcgtggaatc gcaactactt cattctcctc
      961 tcaacgggcg tacttctgag tagttttgct tccaccgcaa acccgcaaat gttcgccctc
     1021 gcccgtgaac acgccgacag aacaggccgt gagacggtca tgttcagtac atttttacgt
     1081 gctcagatct cgcttgcctg ggttatcggg ccaccgctcg cttatgaact ggcaatggga
     1141 tttagtttta aagtgatgta tctcaccgct gccatcgcat ttgttgtttg cggactgata
     1201 gtctggttgt ttttgccatc aatacaaaga aatattcctg tcgttaccca acccatagaa
     1261 attttaccct ccacccacag gaagcgggat acgcggctac tttttgtggt ctgttcaatg
     1321 atgtgggcgg cgaataatct ctacatgata aatatgccgc tatttattat tgatgaacta
     1381 catctaaccg ataaactggc tggagaaatg attggtatcg ctgccggtct ggaaattccg
     1441 atgatgttaa tcgcaggcta ttacatgaaa cgtattggca agcgactatt aatgctcatt
     1501 gctatcgtga gtggtatgtg tttttacgcc agcgtactca tggcgacgac tccggcggtt
     1561 gagctggaat tgcaaattct taatgccatc ttccttggta ttctctgtgg tatcggcatg
     1621 ctttattttc aggacctgat gcctgaaaaa ataggctctg cgacaacgtt atatgcaaat
     1681 acttcacgcg tcggctggat tatcgccggc tctgttgacg gaattatggt tgaaatctgg
     1741 agctaccatg cgttgttctg gctggcgata gggatgttgg gtattgcgat gatttgcttg
     1801 ctgtttatta aagatattta gctcaccagt ctggactaag aaaaatcggc gtggcgcaaa
     1861 cagagccatc ctcgccattt ttgctatcat gcctgcatac ataaacgaca aaacagtatg
     1921 cagagggaaa aatgggttcc accagaaagg ggatgctgaa cgttctgatt gccgccgtgt
     1981 tgtggggaag ttcaggggtc tgcgcgcaat acatcatggt tcaaagccag atgtcgtcgc
     2041 agtttttgac tatgacgcgt ttgatattcg ccggtttgat tctgctgacg ctgtcatttg
     2101 ttcatggcga taaaatcttt tctattatta acaatcacaa agatgccatt agtctgctca
     2161 ttttttccgt ggttggcgcg ctaaccgtac agctcacctt tttactgacc attgaaaaat
     2221 cgaatgctgc cacggcaacg gtgctgcaat tcctctcacc gacgattatc gtcgcctggt
     2281 tctcactggt gcgtaaatcc cgcccgggca ttctggtttt ttgcgctatt ttgacatcgc
     2341 tggtcgggac ttttttattg gtgacacacg gtaatccgac atcattatcg atctctcctg
     2401 ccgcgttatt ctggggcatt gcctcggcat ttgctgctgc attctatacc acctatccct
     2461 caacgctgat tgcccgctat ggcacgttac cagtcgtcgg ctggagtatg ctgattggcg
     2521 gtctgattct gttgcctttt tatgccagac aaggaacaaa ctttgtcgtt aacggcagtt
     2581 tgattctggc gtttttttat ttggtggtca ttggtacatc tctgacattt agcctgtacc
     2641 tgaaaggagc acaattaatt ggcggtccaa aagccagcat tttaagctgt gcagaaccat
     2701 taagtagcgc gctgctctct ttgctgttgc tggggatcac gttcacattg ccggactggc
     2761 tgggaacgct gctgattctg tcatcggtaa ttttgatttc aatggattcc cgtcgccgcg
     2821 ccagaaaaat aaatcgtcca gcgcggcatg agtgagaatt accagccagg caccgcgcca
     2881 ccgttaaaga tggtttctgc cgcttttgcg acttcgggtg attgataaga ttgcagaaat
     2941 tccttcacgt tttctgcatt cttattatct tcccgtgcca ccaaaatatt cacatacggc
     3001 gaatttttat cttcaataaa tacgctgtcg tgcaccggag aaagcccggt ctgctgaatg
     3061 taagtggtgc tgataatcgc cacatcaact ttgggatcat ccagtacgcg cgggagctgc
     3121 gccccctcga gttccataat ccgcaaatga cgaggattct cggttatatc cagcgcggta
     3181 ggtagtaacc cttttccctc tttcagagta attagcttct ctttttgcaa cagtaaaagc
     3241 gcgcgaccaa ggttggtcgg atcgttgggg atcgccactg tcgcgccttc ttttatttgc
     3301 gccacggttt tgattttttt ggaataaccc gccatgggga agacaaacgt atttcccacc
     3361 gctaccagtt tataaccatg cgcctgatta tcttgttcaa gaaagggtcg atgctggaag
     3421 acgttcgcat caagctcgcc atgatttgtc gcatcgttcg gtagcaacga accgctaaaa
     3481 cctaccagtt caacatcgag gccatatttc tcttttgcca cctttttagc gacttccgcg
     3541 acatcttgtt ctgcgccatt tataacgcca actttaatgt gttttgcatc gctgctgctc
     3601 tggtcgcaac ctgctagcag aattcctgcc agcaataatg cggcccccgc ccgtagatga
     3661 tgtgttgtca gtttcatttt ttatcctttt tttaatcatt aactgactat aatggcaaga
     3721 tcactacgat ttaaaaaacg aaaagctatc gaatagaaga aaaaggtata aaaaactggc
     3781 acttcttact gaccacgcaa acacctttcg tacagcatga tgagagcggc aaaagcaaac
     3841 tacacttatg cttcattcga tgcacatttg cagaaggttg ccgctatgaa gctaacgatg
     3901 ctactcatga ttaccgtatt cctgactttt ccagccattt ctcaggccga gtcgccattc
     3961 agttcgctac agtcggcaaa agagaaaacc acggtattgc aagatttacg taaaatttgc
     4021 acgccacagg cgtcattatc agacgaagcg tgggaaaaat taatgttgtc tgatgagagt
     4081 aacaaacaac atatccgtga agctattgtg gcgatggagc gtaataatca gaataactac
     4141 tgggaagcgc tgggtaaggt ggaatgcccg gatatgtaaa tttcgcacac ctccgcgctc
     4201 aggccagcag ccgctgactt aagctcgcac atcagtaaat cacctgcttt ttcaaattcc
     4261 aatcaaaatt gaaccagttc atcaggattg tggataaatt tccctctaca tta
//
LOCUS       DAZUCE010000315         4282 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZUCE010000315 DAZUCE010000000
VERSION     DAZUCE010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.317"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            55..849
                     /gene="hyxR"
                     /locus_tag="ACHY03_004795"
     CDS             55..849
                     /gene="hyxR"
                     /locus_tag="ACHY03_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HFS3907923.1"
                     /translation="MKKPLVIISACQFTRLALESLIPTDRYLVRVYSNVTTEVEFILK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYYGFP
                     RISTLLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALRKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVKNMVSGILYPEKVAVGESINSDAISGKVLSRQRKNISKKRPGLL"
     gene            1003..1764
                     /gene="hyxA"
                     /locus_tag="ACHY03_004796"
     CDS             1003..1764
                     /gene="hyxA"
                     /locus_tag="ACHY03_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HFS3907924.1"
                     /translation="MRFVLFCPSGIVPAQFVALSTGSVCNITCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANTSGGFSGLKHAEILPVIDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1806..4103
                     /gene="hyxB"
                     /locus_tag="ACHY03_004797"
     CDS             1806..4103
                     /gene="hyxB"
                     /locus_tag="ACHY03_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter HyxB"
                     /protein_id="HFS3907925.1"
                     /translation="MKNNKAFYRSALATAIVMALSAPAFAADNAVSTTSVTLDKNNTT
                     LNQDVVINGDKKITAVTIETSDSDKDLNVTFGGHDITAASTVNQDFVEGVKVSGNKNV
                     VINATDSTITAQGEGTYVRTAMVIDSTGDVVVNGGNFVAKNEKGSATGISLEGTKGNN
                     VTLNGTTINAQGNKSSSNASTAIFAQKGSLLNGFNGDATDNITLAGSNIINGRIETIL
                     IAQENKGTHTVNLNIKDGSVIGAANNKQTIYASASAQGTGSATQNLNLSVADSTIYSD
                     VLALSESENSAATTTNVNMNVARSYWEGNAYTFNSGDKAGSNLDINLSDSSVWKGKVS
                     GAGNASVSLQNESVWNVTGSSTVDALAVKDSTVNITKATVNTGTFASQNGTLIVDASS
                     ENTLDISGKASGDLRVYSAGSLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQG
                     ADGNFYFVKNTHKASNASSVIQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWI
                     QYFGGKQKHTTAGNASYDLDVNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSK
                     GDTEGYSFHAYLSRQYNNGIFIDTAAQFGHYSNTADVRLMNGGGTIKVDFNTNGFGAM
                     VKGGYTWEDGNGLFIQPYAKLSALTLEGVDYQLNGVNVHSDSYNSVLGEAGTRVGYDF
                     AVGNASVKPYLNLAALNEFSDGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAY
                     ASLDYTKGDDIENPLQGVVGINVTW"
BASE COUNT         1176 a          928 c         1072 g         1106 t
ORIGIN      
        1 tgacattttg aatcaaaatg tcaggtttcg tagcaatggc gtggacgagt ctgaatgaag
       61 aagcctctgg ttattatcag tgcctgtcag ttcaccaggc tggcactgga gtctctcatc
      121 ccaacggaca ggtatctcgt gcgggtgtac agcaacgtca ctacagaggt tgaattcatt
      181 ttaaagacct cctgtggcta cctgttagct gatatccctt cgcttccttc gggggagttg
      241 gttcttctgg cttgtcttgt ccgcttgcgg agtgatgccg ggcaatggca ggtctgcctg
      301 accggtgact ccgactggtt cgataatgct cctgctgcaa gtctgtacta tggcttccca
      361 cgcataagca ccctgctgac agcgagtcgt tatcagaatc aaattatccg ctggttacta
      421 cgtcctttgc ccggcgatat gagtgatttc tggctgctga ctgcacggga gttgagcgtg
      481 cttaagattc tgatgcaggg catgagtttc agtgagattg cccgttatga acaacgcagt
      541 tcaaagactc tgcatgcgat tgccacacgg gcattaagga aacttggact gggaacactg
      601 agcgatttca ggctgctcta tacgggatgc ggtgacagca gagtgaaacg gaatatacgg
      661 ctacatgcaa gatatgtcgg acaacattcc gctcatcgac tcactcagta tgtgaagaac
      721 atggtatccg gaatccttta tccagagaaa gttgccgttg gtgaatcaat aaattctgac
      781 gccatttccg gaaaagtact ttccagacaa agaaaaaata tatctaaaaa aagaccagga
      841 ttgttatgac acagcagcgt tgttactacc acccactgcg gcgcatacgg actcgctaca
      901 gtgtaggagc caaaatattt ctactgctct gggctgcagg attgggaggc acgctgtaca
      961 gcctgtacag cgtgctggcg tactgacatc tgtgagggaa ggatgcgttt cgttcttttc
     1021 tgtccctcag gaatcgtacc agcgcagttc gtagctctat caaccggttc tgtctgcaat
     1081 attacctgca tcagaacaga agaggagtta cgcaataaat tgcggcacag gccacagagt
     1141 gtcgtgatat cggcagggcg acctgccgaa tgcgctgaaa tgtggttccg attttatcgg
     1201 gatcactcat tcgtggtggt gttgtgcgtc gcgccctttt ttctgccacc ggatgtgagt
     1261 atttctggcg ttctcaaaaa cctgaggtta ctgaaaccag gaatgtcagt agagcatgtc
     1321 atcagtattg caaatacctc tggtgggttt tcagggctga aacatgcaga aattttgcca
     1381 gttatagaca gttactctgt ctttatgaaa gaggtgaata accgcacaaa gacaatcgtc
     1441 atgtcagaac gcttcccgga aaagcaaaag aaggtgttgt cgttgcttct tgccggacac
     1501 agctgggaat acagtgcgca gtttctcaaa acggggataa gacagatatg gcttgccgag
     1561 cagtcgctaa aaaagcgttg gggaataccg gacagcatgt cattgcggga agccctgctg
     1621 ctgagcagca ataaatttca cggcgatgga aatgcattgg aaaaaacgaa tgccatgaca
     1681 ttgcgtgaaa acggaaatac caacaggtat tccgtggtgg taaatgcagg gacgcaggct
     1741 ttatctctgc ataaatataa ataagtggga aattgaaatt agttaccaga atggagaatt
     1801 tcttcatgaa aaacaataag gcattttacc gcagcgcatt agcgacagct attgttatgg
     1861 ctctttctgc accagcattc gctgctgata acgcggtatc aactacgtcg gttacgttgg
     1921 ataaaaataa cacgactctg aatcaagatg ttgttattaa cggtgataaa aagattacag
     1981 ccgtaacaat tgaaacgtca gattcagata aagaccttaa tgttactttt ggcggtcatg
     2041 atattaccgc cgcatcaacg gtaaaccaag atttcgttga aggtgtaaaa gttagtggta
     2101 acaaaaatgt tgtgattaat gctacagact ccaccatcac agctcaaggt gaaggcacct
     2161 atgtccggac tgcaatggtc attgattcaa ctggcgatgt tgttgttaat ggcggtaatt
     2221 tcgttgcaaa aaatgaaaaa ggtagtgcga cagggatatc tctggaaggg accaagggaa
     2281 ataatgtcac gcttaatggc acaaccataa atgctcaagg taataagagt tccagcaacg
     2341 cctccacggc aatttttgct caaaagggta gcttattgaa tggttttaac ggtgatgcaa
     2401 cagacaacat tacccttgct ggctcaaata ttattaatgg tcggattgaa acaatactta
     2461 ttgctcagga gaataagggt actcatacag tcaacctgaa tattaaggat ggctcagtaa
     2521 ttggggcggc taataataaa caaacaattt atgcctctgc ttcggcacaa ggcacaggtt
     2581 cagcaacgca aaatttaaat ttgtctgttg ctgattcaac catctactct gatgtcctgg
     2641 ccctttctga aagcgagaat tcagccgcta ccacaacaaa tgtaaacatg aacgttgccc
     2701 gctcttactg ggaaggtaat gcttatactt tcaatagcgg cgataaagcg ggtagtaatc
     2761 tggatataaa tctttccgat agttcagtct ggaaaggcaa agtttcaggg gcaggaaatg
     2821 ccagtgtatc tctgcaaaac gagtctgtct ggaatgttac gggatcctca actgttgatg
     2881 ctctggcagt aaaagacagt acggttaata tcacgaaggc tacagtcaat actggcacgt
     2941 ttgcttctca aaacggcacg ctgattgttg atgcctcttc tgaaaacacc ctggatatca
     3001 gcggtaaagc gagcggtgac ttgcgtgttt acagtgcggg ttcattggat cttatcaatg
     3061 aacaaacggc atttatttct accggcaaag acagcactct aaaagccaca ggcacaacgg
     3121 aaggtggtct gtatcaatat gacctgacac agggagctga tggtaacttt tatttcgtaa
     3181 aaaacacgca taaagcatcc aacgccagct ccgtgattca ggcaatggca gctgctccgg
     3241 ctaacgtcgc taatctgcag gctgacacgc tctcagcccg tcaggatgct gtccgtctga
     3301 gcgaaaatga caagggcggc gtatggattc agtactttgg cggtaaacag aaacatacca
     3361 ccgcgggaaa tgcatcctat gacctggatg taaacggtgt aatgctgggt ggtgataccc
     3421 gcttcatgac tgaagatggt agctggctgg ccggtgtggc gatgtcttct gcgaaaggtg
     3481 acatgaccac catgcagagc aaaggcgaca ctgaaggtta cagcttccac gcttacctga
     3541 gccgccagta taacaacggt atcttcattg atactgctgc acagtttggt cactacagca
     3601 acacggcaga tgttcgtctg atgaatggtg gcggtaccat caaagttgac tttaacacca
     3661 atggtttcgg tgcgatggtt aaaggcggtt acacctggga agacggtaat ggcctgttta
     3721 ttcagccata tgccaaactg tctgcgctga cgctggaagg tgtggattat caactcaacg
     3781 gcgtgaacgt tcattctgac agctataact ctgtgctggg tgaggccggt acgcgcgtgg
     3841 gttatgactt cgctgtgggc aacgcgagcg ttaaacctta tctgaatctg gccgcactga
     3901 acgaattctc tgatggcaac aaagtccgtc tgggtgatga gtctgtcaat gccagcattg
     3961 acggtgcagc attccgcgtg ggtgcaggtg tacaggctga tatcaccaaa aacatgggag
     4021 catatgcaag ccttgactac accaaaggtg acgacattga gaatccgcta cagggtgtag
     4081 ttggtatcaa tgtgacctgg taagaataaa gcagtcggtt cccctgtcga ctgttatacc
     4141 ccctgcaggc agcagtatgc tgcctgcagg ctataaatat ccagtattca tgatgctggc
     4201 tttttgctga tatttaaccc gaacattcct gatacagact attaattcat ttatctgcgg
     4261 cagattttat tttctggata ta
//
LOCUS       DAZUCE010000316         4239 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZUCE010000316 DAZUCE010000000
VERSION     DAZUCE010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.318"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(214..882)
                     /gene="artM"
                     /locus_tag="ACHY03_004798"
     CDS             complement(214..882)
                     /gene="artM"
                     /locus_tag="ACHY03_004798"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFS3907926.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(882..1598)
                     /gene="artQ"
                     /locus_tag="ACHY03_004799"
     CDS             complement(882..1598)
                     /gene="artQ"
                     /locus_tag="ACHY03_004799"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFS3907927.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1605..2336)
                     /gene="artI"
                     /locus_tag="ACHY03_004800"
     CDS             complement(1605..2336)
                     /gene="artI"
                     /locus_tag="ACHY03_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFS3907928.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYKTIYNKWFQK"
     gene            complement(2354..3082)
                     /gene="artP"
                     /locus_tag="ACHY03_004801"
     CDS             complement(2354..3082)
                     /gene="artP"
                     /locus_tag="ACHY03_004801"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFS3907929.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3300..3815)
                     /locus_tag="ACHY03_004802"
     CDS             complement(3300..3815)
                     /locus_tag="ACHY03_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFS3907930.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         1091 a         1079 c         1050 g         1019 t
ORIGIN      
        1 aaaaaaagac ggacaactta gtgagttgtc cgtctttatt ataagaattt atgcactatg
       61 taggccggat aaggcgtcca cgccgcatcc ggcacaggca ccgtgctgat gtctgatgcg
      121 acgctggcgc gtcttatcag acctacaaat catccagcta atgtaggccg gataaggcgt
      181 ttacgccgca tccggcatca gcacgcagct acattaattc cgccgttcaa atgccagtgc
      241 tttgcgctcg atcagacgca tcatcagcgt cagcaggccg ttgacgacca ggtaaataat
      301 ccctgccgca ccgaacacca tcacatcgta ggtgcgcccg tacaacaact ggctgtaacc
      361 catcacttcc atcagcgtaa tggtgtaagc cagagaggta cttttgaaca ccagcaccac
      421 ttcgttggaa taagaagaga gtgagcgttt aaaggcatac ggcagcagga tcgccagcgt
      481 atctttttta ctcattccca gagcgctaca ggattgccat tgaccttccg ggatcgcacg
      541 aatcgcaccg taaaacagct gcgtggtgta cgccgcactg ttcagcgata acgcaatcaa
      601 tgcacataac cacggttctg acaacaaatg ccacagtgcc ggatactcct gcaacgtcgg
      661 aaactggccc ggcccgtaat aaatcaggaa gatctgcacc agcagcggcg taccggtaaa
      721 cagcgtgata taaccccgca ccagccacac cagcaccggc gttttcagcg tcaggatgat
      781 ggtaaaaatc aatgccagaa tcagcgccac aatcagcgag gcaaccgtca gcgtcaggct
      841 ggtgtgcagc cctttcatca gttcgggtaa atactcaaac attagctggg cctccgctca
      901 aaacgtgtcg cgcgcaggtc aatgcgtttg agaatgtact gactgagcag ggtgatcacc
      961 aggtaaatcg ccgccgccac aatgtaccag gtaaaaggtt cctgggtacg agtagcaatg
     1021 cttttggttt gtagcattaa atcattcaca ctaatcaaac tgaccagcgc ggtatctttc
     1081 agcagcacca gccactggtt accgaggcca ggcagcgcat gacgccacat ctgcggcatc
     1141 accagacgga aaaagatagc cgatttcgac agccccagcg cctggccgga ttcccactga
     1201 cccatcggca ccgctttcaa cgcgccgcgc agcgtttgcg aggcataggc ggcatacagc
     1261 agtgacagag cgatgacacc acaaaggaac gggctaacgt cgaagttctc aatgtccatc
     1321 tgcactggga tctgcacgaa cccaagattg atagtgaagc catccgaaag cgtcagcagc
     1381 agctgcgagg agccaaaata gataaacagc accaccagaa tttctggcag gccacgcaga
     1441 atggttacca gcgctgaacc tgcccacgcg acaggacgcc attttgccga ctcccatacc
     1501 gcaaagaaca tcgccagcgc cagtccgaca atcaatgcac aaacggcaag gccgacggtc
     1561 atcccggcgg cgcttgctaa aggaaaaaat tcattcatca ggaattactt ctggaaccat
     1621 ttgttgtaga tggttttgta agtgccatct ttcttcactt tttccagcgc agtgttgagt
     1681 ttctgctgca gctcagtgtt gccctgacgt accgcgatgc cgaggccagt accgaagtaa
     1741 tctttatcgg tcactttatc gcccactgcc gccagtttcg ggttatcttt cagccattcg
     1801 gttaccactg cggtgtcacc aaatacgctg tcgatacgac cattttgcag atccagtttt
     1861 gcgttctggt agctgtcata cggaacggtg gtgatttccg ggtgcttatc cataatgaac
     1921 ttctggtgtg tcgtcccgtt ctgaacgccg acttttttgc ctttcagctg atcaacactg
     1981 gtgtatttgc cttgctgacc cacaaacagg gcagagttgt catagtacgg ggtggtaaac
     2041 agcacctgct tttcacgctc cggagtgata tccatgcccg ccatcacggc ttctacgcga
     2101 cggaatttca ggcttgggat caggctgtca aacgcctggt tagagaaagt acaggttgca
     2161 tcaatctctt tacacagtgc ctgtgccagg tcgacgtcaa aaccaacgat ctggttgttt
     2221 gcatcaatcg attcaaacgg aggataggag gcttcggtag caaaacgaat ggtttcggca
     2281 gctgtggcgg aaagactaaa acctgcaatt aacgcggcaa tcagaacttt tttcattgtt
     2341 gttatcccga atcttagtga gagagatagt ttttaaatgc ttcggtttgc ggctcggtaa
     2401 agcagctcgc gtcgccttgt tctacgatat gaccattttc catatacacc actcggctgg
     2461 cggttttacg cgccacttca acttcgtggg tgacgatcac ctgggtaata ttcgtttctg
     2521 ccagctcacg aatgatgctg acgatttgtg cggtaatttc cgggtccagt gcggcggtcg
     2581 gttcatcaaa cagcagtacc tgcggttcca tcattaacgc acgggcaata gcaacacgct
     2641 gctgctgacc accagaaaga tgcagcgggt aacgatcgct ataaggtttg agacgcagac
     2701 gttccagcag tttttctgca cgggccagcg cctgatcttt actcaacccc agtacacggc
     2761 agggcgcttc aatcaggttt tgctgcacgg tcagatgcgg ccacaggttg tattgctgaa
     2821 acaccatgcc aacgttacga cgcaaatcgc gaatcgcttt gtctgagggt gttttggtga
     2881 aatcgaaatg gttgcctgca atgttgagcg taccggagcg cggcatctca agcagattga
     2941 gtacacgcag cagcgagctt ttacccgcgc cgctggggcc aagtaacacc agcgtttcgc
     3001 cctgtgggca atccagcgtg atatcgaaca gcgcctgatg cgcgccgtag aagcaattaa
     3061 tgccgtttaa ttgaatactc attgacactc gtatactggc agtctgatag ctattgaggt
     3121 cgaagatagt acctttgaca gaataattat gcaatatttc tgctttaaaa gttaaaagca
     3181 aagcgcatta ttcaataaac atagcacaaa ataacggggg cggtggtcgg cgagcataaa
     3241 tgtcggcatt cctcacgaaa tgccggacaa tttacggggt ttattggttg atcaaggcgt
     3301 tagcgattct cgatggactg acggagcgta cccgccgtgg catgaacgct accgcctaag
     3361 tagcgcacat cgtcgatcac ccaacactgg ccttcctgaa tcattaacac ttcatcctgc
     3421 cagccctggt caccctgctt gagatccacg cgcaatggaa tgttacgggc atcacgattc
     3481 gggatagtcg atgcactggc aacgtgggcg ctgtctggca aggtggttcg actggagaac
     3541 ggatcactgc ttaacaattc acgatgacta ttatcgcggc tggcatcgct aagtagggtt
     3601 gccagtttgt cactcaaata ggggcgcagg gcggtaatgt cattgctgcg atgttgaatg
     3661 cggtagtcat agaattgttg ggccacgctg tcaggccccc cctggacaca ggaaccggtg
     3721 cggggaccgt tatctttata agctggcgtg acggtggtac aggcactcag cagcagtgca
     3781 catggaataa gtagagatag tgaacggtag cgcataatca tttccttatt agcttgctgt
     3841 ctgcgtcaca ctacgaccaa acgctatcag attatttaat acattgtttt taatacaatt
     3901 ctgaaacaat ctgaacacct gattgggttt gagtccattg ctggctaccc actcaaggtt
     3961 gctcgtagct gacttactta aattacccaa acaatagaca gtgtaaacca tttgtgaggt
     4021 tgagcgtgtt atatggctga aatgatcatt taaggtaaat atgatagttc tgaaatggtg
     4081 gtataaatcc tcctgtagag gttaagagca tctgcaagaa gccagtttca catttataac
     4141 gcgtgataaa tgaacgcggt tattgtctaa tcattacaaa tttttcacat gcgataagag
     4201 atcctgctat taagatgatt gccaaaatta cgttgtatt
//
LOCUS       DAZUCE010000317         4222 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZUCE010000317 DAZUCE010000000
VERSION     DAZUCE010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.319"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..209)
                     /locus_tag="ACHY03_004803"
     CDS             complement(<1..209)
                     /locus_tag="ACHY03_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS3907931.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHY03_004804"
     tRNA            complement(324..399)
                     /locus_tag="ACHY03_004804"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHY03_004805"
     tRNA            complement(406..480)
                     /locus_tag="ACHY03_004805"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHY03_004806"
     tRNA            complement(597..681)
                     /locus_tag="ACHY03_004806"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHY03_004807"
     tRNA            complement(690..765)
                     /locus_tag="ACHY03_004807"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHY03_004808"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHY03_004808"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFS3907932.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHY03_004809"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHY03_004809"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFS3907933.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHY03_004810"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHY03_004810"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFS3907934.1"
                     /translation="MNHSLKSWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1082 a         1047 c          947 g         1146 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggctttccg gaatcgtatg atatgcatcg cctggtcaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaaggattaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccga gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt tcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccgtcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaaacgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcactt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcatcaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttctccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccaggatt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccat
     4201 cctgacggat ggcctttcgc tt
//
LOCUS       DAZUCE010000318         4207 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZUCE010000318 DAZUCE010000000
VERSION     DAZUCE010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.320"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..2572)
                     /locus_tag="ACHY03_004811"
     CDS             complement(<1..2572)
                     /locus_tag="ACHY03_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS3907935.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATRATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSEMNAAASQKSA
                     ATSASTATTKASEAATSARDASASKVAAKSSETSAASSAGSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSAANSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLATAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     N"
     gene            complement(2637..3236)
                     /locus_tag="ACHY03_004812"
     CDS             complement(2637..3236)
                     /locus_tag="ACHY03_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFS3907936.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWYEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(3305..>4207)
                     /locus_tag="ACHY03_004813"
     CDS             complement(3305..>4207)
                     /locus_tag="ACHY03_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS3907937.1"
                     /translation="QTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRL
                     YIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKYL
                     TAPTITSGGNPPAFSLTPDGRLTAKNADISGSVNANAGTLNNVTINENCRVLGKLSAN
                     QIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERE
                     NNDIYSSCRLIVKKNGAEIYNRTALDNTLVYTGVIDMPAGRGHMTLEFSVSAWLVNDW
                     YPTASISDLLVVVMKKSTAGITIS"
BASE COUNT          847 a         1216 c         1063 g         1081 t
ORIGIN      
        1 tgttattcgt ggatttagtg ccgtaatcaa acgacgatgt ggttttcgtc cccaaatccg
       61 tactggatgc gctggcgctg tgggtgtgcg atttaatgcc gtcctgttcc tgagacaata
      121 cggcccgacc actggcaggt ttgcccttaa tcgtccagcc acgcatatca gggatcacgc
      181 ctgacggata agcggttgca agtttcgggt aagcagattt gtcaaaagtc tgcccctgca
      241 tcagggcata accagacgga acggtatctg atggccacgg gattggtgcg ccaggcggat
      301 aaaactgctc tgatggcgta tagagtgaat aaactgtacc gtccgttaac ccttccggct
      361 tattagcaga atatgctggt gacgtatgaa tagtcacgct ggcattactg gtataatccc
      421 attgaatatt tacaccagtc gcataatttc cgattgcaac gtaaatatcg taagtatcac
      481 cagatgtatt gacccaggca aaatttgtaa accctgtcga tgtgcgctgc cataaagcac
      541 cagtaatccc cttcggatta ccattacctg cacgcaaaac aagttcagat atacctgcct
      601 gttgaggtga ccccacgtta aatcccgcgc caccaatcaa cgtaattgaa acaacagaac
      661 tcgcctgtgg catggttacc gttgctaatt tgaaccaacc agcaccaccg ctgaatgaca
      721 ttgttgttga gttaagcgta ccaatatctt tcggcgtcag tgttatatcc gctgaaagcg
      781 ccttaccatt caccttacgg gcagaaggta cccgaccatt cgcattatca ttagctgctt
      841 tcactgcttt cggtgtcgcg gcaagcgttt cagatgcgct gttggttgca ctactgagct
      901 ggacaattcc tttttgtgct gtcgtagcgt cctgagcggt atatttcccg ttagcaaggt
      961 cataggctgc ctttaccgac tttggcgttg ccgccagcat ttcagatgtg ctgttggtcg
     1021 cgctgctgag ctgaactatc cctttctgtg ctgtcgttgc atcctgtgcg gtgtatttcc
     1081 cgttagccag gtcatacgcg gccttaactg cttttggcgt tgccgccagt gactcggaag
     1141 tgctgttagc cgcactgctg agctgtacta tccccttttt cgtcgtgctc gcatcctcaa
     1201 gcgccacggc ggatgcaata tcctctgccc gttttgccgc tgtctcagcg cgcgttgctg
     1261 cagattccgc cgtacttttg ctctgagctg ctgccgtcgc actaccagct gcctctgtcg
     1321 ccttcgtgga tgccgtcgtg gcgctgccct tcgctgctga cgcctgtctg gtcgcctcat
     1381 cttttgaagc agacgccgat gatgccgatg acgccgccga actggcggac gatgccgctg
     1441 ccgtttttga ggattctgca cgggtttccg acgctttcgc gttcgtttcg gatgtcttcg
     1501 ctgcggaagc tgacctcgct gctgccgtgg cctgctcagt ggcttcgcca gccttcgttg
     1561 tggctgttga agcggatgat gcggcgcttt ctgccgattt tccggcggcg gtggcactgg
     1621 ctgaggcctg cccggcactt gttgacgcgg cactggcaga cgacgcagcc gctgtttttg
     1681 agcctgccgc agctgaggcg ctctgtcccg ctgccgtctc agaagacctg gcgttcgtct
     1741 cagacgtttt tgcggccttc gcggaatttc ctgccgccgt tgccgaggaa gctgcactgc
     1801 cggcgctcga ggcagcgctc gtttctgatg attttgccgc cacttttgaa gccgacgcat
     1861 cccgggctga ggtggcagct tctgacgctt tcgtggtcgc ggtggatgca gatgtggccg
     1921 cagatttttg tgatgctgcg gcattcattt ccgacgtttt cgctgcacct gcactggtgg
     1981 ctgccgcgct ttttgaagac tctgcggcgg cagcactttt tgatgcttca gtggcctttg
     2041 ttgatgctgt tcctgcgctg gaagacgctg actgagccga cgacgcggcc tgtccggctg
     2101 acgtgctggc tgcacgtgct gagcctgcag catcagtcgc acgggttgcc gcctcacggg
     2161 ctgatgtgct ggcatcgctg gctgacttct tcgcggctgc cgtgttctgc gccactgcgg
     2221 acgcgttacg cgccacctct tccaccatca gttcaaaacg gcgcagtgcc tccggacggg
     2281 catcatcctc cgtcatggca ccgagaaaat cattcagcgt accgggttga gaatcttcat
     2341 acacggtgat ggtcccggca tgtgacggcg ggaatccttc caccaacaga ataacgctgt
     2401 actgaccgta ctcaacgtcc atgctgtaac gcccggcttc atccggattt tctgaggcca
     2461 gcgtgttcac caccaccgtg gtgctgttac gttttgcttt cagctggatt gtgcagttct
     2521 gtaccggttt tcctgtgccg tctttcagta cacctgaaat ctttactgcc atattcaccc
     2581 cacaaaaaag cccgcctgaa ccggcgggct gtcataacac tgtgttacct ggctaatcag
     2641 aatttataac cgacacccac gatgaaaccg tcagtgcgcc agtcaccact gccggaacct
     2701 tcataagcga catcaatggc cacggactcg gtcgggttaa actgcacgcc agccccccac
     2761 gccagagacg tgttgctgtg gcgaccgtca tcacttccgg tcagcacatc gtgcgttttc
     2821 cccttattgt cagttacgcg gatataatcc ccggaaaaag tcgacacacg gctgtaagcc
     2881 acacccgcca tcgcatacgc gctgaaccat tcattcacgc gtacagacgg ccccgccatc
     2941 acgctgaacc agcggttacg cacggaatct tcataccagc gggtatcgct gtaacgggtc
     3001 agctggcgat tcttgtctcc tgcatagctg aacgacgtca ccatccccag tgtgtccgta
     3061 aactcataac ggtatttcac gttaatcccg ttcagatcat cactgccggg aacgttcgtc
     3121 cgggcatgaa gataccccgc gctcagcgtg gactgatgtt cagacgccca tgcaggcgca
     3181 ccggatacgg ccagacagat ggctgcggac aaaatggctg cacaaacttt acgcataatt
     3241 acctctcgct tttctgcaat aaaaaaggcg ccagaaatgg cgcccgcata tgggttatga
     3301 aaattcagct aatcgtgata cctgctgtgg atttcttcat caccacaacc agcaaatcac
     3361 tgatactggc tgtgggatac cagtcattta ccagccacgc tgataccgaa aactccagcg
     3421 tcatgtgacc gcgaccagca ggcatatcaa taacacctgt ataaaccagc gtattatcca
     3481 gcgcggtacg gttataaatt tcagcaccgt ttttcttcac tatcaggcgg catgacgaat
     3541 aaatatcgtt attctcccgc tcatgtttag cgccacgaaa cgccaccgcg ggaataacaa
     3601 tctgccggtc aaacggctga tcgtcataaa ccctgacggt aatggtccct gatggccacc
     3661 gctccggtgc acgggagtcc cgggggaaag ctttgcccac tgttttaacg agatcgcctt
     3721 caatctggtt cgcagacagt tttcccagaa cccgacagtt ttcatttatc gtgacattgt
     3781 tgagcgtccc ggcgttcgca ttcacactgc cactgatatc cgcatttttc gccgtcagtc
     3841 gcccgtccgg tgtaagggaa aatgcaggag gattaccgcc gctggtaatg gtgggagccg
     3901 tcagatattt caggaacacg tcgttcatga atatctgatc accctgacca acaaacatcg
     3961 gctttgtgtt gccattcgca ggattaatca tcgcaatcct gtctgccgcc agtagcacct
     4021 gactctgcat tcctgctggc gtattctcaa taccggcacc gatacccgca atataaaggc
     4081 gtccgtcctg catctgctgc agtttcacgg cccacatgct gttcaggtta ttatttgtat
     4141 caacctgaac cttctgtatc tgctggatcg ctgcactctg gtcttccagt ttcttattga
     4201 cggtctg
//
LOCUS       DAZUCE010000319         4165 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZUCE010000319 DAZUCE010000000
VERSION     DAZUCE010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.321"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1421
                     /locus_tag="ACHY03_004814"
     CDS             <1..1421
                     /locus_tag="ACHY03_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFS3907938.1"
                     /translation="KFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGS
                     LYTFYKTSAQNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKAN
                     QESIENNPFAPSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGHKPACDSRVICV
                     PQGSYDIDDKVGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNP
                     FLKPERAETWQAGFNIDTRDLVVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCS
                     MAEVIGNDWEGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTD
                     QPTSIASTYFGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQD
                     EHTGAIIKQDLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLG
                     DETHPANSARGRTWIFGGDIRF"
     gene            1490..2665
                     /gene="senB"
                     /locus_tag="ACHY03_004815"
     CDS             1490..2665
                     /gene="senB"
                     /locus_tag="ACHY03_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFS3907939.1"
                     /translation="MNIFTLSKTPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSGDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(3295..3753)
                     /locus_tag="ACHY03_004816"
                     /pseudo
     CDS             complement(3295..3753)
                     /locus_tag="ACHY03_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612075.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         1154 a          981 c          983 g         1047 t
ORIGIN      
        1 ataaatttac acgtcgtgat ataaccagtg acgactatta cattaaatat cattacaccc
       61 ctttttctga attaattgac tttaacgtaa cggccagtac cagtcgcggt aatcaaaagt
      121 atcgtgatgg ctcgctgtat actttctaca aaacctcagc gcaaaatcgt tctgacgcgc
      181 tggatatcaa caataccagc cggttcactg tcgcggacaa tgacctggag tttatgctgg
      241 gcagcaaact gatgcgtacc cgctatgacc ggaccattca ctcagcggcg ggcgacccga
      301 aagcgaatca ggaatcgatc gagaacaatc cgttcgcacc ctccggccag caggatattt
      361 cagcgctgta taccgggctg aaggttacgc gcggcatctg ggaggcagat ttcaatctca
      421 actacacacg taacaggatc acagggcaca agcccgcctg cgattcacgc gttatctgcg
      481 tgccacaggg tagctacgat attgacgata aagtgggtgg cttcaaccct tcagttcagc
      541 tttctgctca ggtaacacca tggcttcagc cgttcattgg ctacagcaaa tccatgcgcg
      601 ccccgaacat ccaggagatg ttcttctcta attcaggagg cgcatccatg aacccattcc
      661 tgaagcctga acgtgcagaa acctggcagg cgggttttaa cattgatacc agagatttag
      721 tggtcgaaca ggatgccctg cgctttaagg ctctggcgta ccgcagcagg atccagaact
      781 acatctacag cgagtcttat ctggtttgtt ctggaggtcg taaatgcagt atggctgagg
      841 tgattggcaa tgactgggag ggcattagcg atgaatacag cgacaatatg tacatctacg
      901 ttaactcggc aagcgacgtt atcgcaaagg gcttcgaact ggagatggat tatgatgcag
      961 gttttgcttt tggccgactc tctttcagcc agcagcaaac agaccagcca acctccatcg
     1021 ccagcaccta ctttggcgca ggggatatga ccgaactgcc cagaaaatac atgacgctgg
     1081 atactggtgt tcgcttcttc gataacgcgt tgaccctggg cactatcata aaatacacag
     1141 gcaaggctcg tcgcctgtcg cctgattttg agcaggacga acataccggc gcaataatca
     1201 aacaggattt gccgcagatc ccaacgatta tcgatctcta tggtacttac gagtacaacc
     1261 gcaacctgac actgaaactt tcggtacaaa acctgatgaa cagagattat tcggaggcgc
     1321 tgaataagct caacatgatg ccaggtcttg gtgacgagac ccacccagcc aattccgcgc
     1381 gtggcagaac atggatattt ggcggggaca ttcgtttctg atgacaattt ccgctaggtt
     1441 cagacaatac gttttctctc ttatgtcaat tttattgcag gaacgaaaaa tgaatatttt
     1501 cactttatcc aaaacaccgc tatacctgtt aatttcacta tttttaccca cgatggccat
     1561 ggctatcgat ccacctgaac gcgaactttc gcgatttgcc ctgaaaacga attaccttca
     1621 gtcccctgat gaaggcgtct atgaactggc gtttgataat gccagtaaaa aggtgtttgc
     1681 agcagtcacc gatcgtgtaa atcgtgaagc caataaaggc tatctgtatt cgtttaattc
     1741 agattcgctg aaagtcgaaa ataaatacac gatgccatac cgggcatttt cgctggcgat
     1801 aaatcaggat aaacatcagc tctatatcgg acacacccag tcagcgtccc tgcgtatcag
     1861 tatgtttgac accccaaccg gcaaactggt aagaaccagc gacaggttaa gttttaaagc
     1921 ggcaaacgct gcagattcgc gttttgagca tttgcgccat atggtttaca gccaggattc
     1981 cgataccctg tttgtgagtt atagcaatat gctgaaaacg gccgagggca tgaagcctct
     2041 gcataagctg ttaatgctcg acgggacgac gcttgcctta aaaggcgagg ttaaggatgc
     2101 ttacaaaggt acagcgtatg gtctgacgat ggatgaaaaa acacagaaaa tctacgttgg
     2161 cggaagagat tacatcaacg aaattgatgc gaaaaatcag acgctgctgc gtaccatccc
     2221 gttgaaagat ccgagaccac aaatcacaag tgtgcagaat ctggcggtgg actccgcttc
     2281 tgaccgtgcc tttgtggtgg tattcgacca tgacgatcgt tccggtacaa aagatggact
     2341 ctatattttt gacttacgcg acggtaaaca gcttggctat gtgcacacag gagccggagc
     2401 taacgcggtg aaatacaatc cgaaatataa cgaactgtat gtcaccaact tcactagcgg
     2461 caccatcagc gtagtggatg ccaccaaata cagcatcacc cgtgaattta acatgccggt
     2521 ctacccaaac cagatggtgt tgtcgggtga tatggatacc ctttacattg gcatcaaaga
     2581 aggctttaac cgcgattggg atcctgatgt gtttgtggaa ggagctaaag aacgtattct
     2641 gagcattgat ttgaaaaagt cgtgatcggg ggaaaacatg ggataaaaac tccgttattc
     2701 cactaaactg gccggactag gaaaagcaaa catctctgtc ctgccggtac gcctgttacg
     2761 ccgctccatg aggctgagga atctccagaa aacgagacag gcacagcatt gtacgatcaa
     2821 ctgatttgtg ccaataattc aatgaaatca cagctatgcc tgttcatggg gttctaggcc
     2881 ggaaccccag atttttccga ttgtttgttt tctaactgat tattcagtag ttttagaggg
     2941 ctgacgctca gtggaacgaa aactcacgtt aagaggtttt gactatgata aaagtcaaag
     3001 taggggtgag tttggatatg gaaattattg tactataagg gcttccccga acagtcaacg
     3061 tcggtattta tggtttgtct gcactacagt taaaaataaa ggggagagac tgcggtacta
     3121 taaacggtca taataaatcg ttatcgattc ggaccctgat gaaagacgcg cgtgaggctc
     3181 ttgttcgtta agcggatacg tatatcctgt acaataatca gatatacctc acggggtcta
     3241 accctttatc atcaacgagt tgcaggtcag atagtcatac ggttgctgcg tgctttagct
     3301 ggctgaatac agtctgaaat catcaccgta atgcaatgat gcccagcaca gccttgcatt
     3361 tttggcggcg atggccacaa cagcgcgcca gtatcctctg cgttcgacca gtgaacagac
     3421 ccaacgactg aatgagtcag tccttttctc agcgccaatc agtactgaac gagcaccctg
     3481 aaccagaagc gttcgcagat acgaatcacc ggcttttgtt atcctgccta gcttcgactt
     3541 tccgccgctg ctgtattgtg atggagtcag tcccagccag gccgccagtt gccgtccatt
     3601 tttaaaatca tgtgcattac cgatacaggc aaccagcgca caggcagttg tgggaccaat
     3661 tccttttagc tccatcagcc gctgactgcg gtgatctgtt cttgctatac gggacaaaat
     3721 tttgtcatat tcagtgatgt tagcttcaat gcggggtcag tttggagatc gtaaaatttt
     3781 gttcgttaag cacattattt aaccaataga tatgcgtaac ggattgattt tgcgtcaacg
     3841 gctatcgctt ttaagttatc gccattgagg tattgcattt aggttatccg atagaggtca
     3901 aatcctctga aaacgccgta tagcacggga gtacacccta ccgcaatagc gcaaccgtac
     3961 aatattgtac gtttcccaaa cgcagcctag gaagcacgtc gtcagtattg gagaaaactt
     4021 acaaacgctc gccgcagccc cagcgaccga acgttagtga gcgagtgggc gaggaagcgg
     4081 aaaagtgtca ggtctgaatt gagcacatac gccagaaatt atatagcaaa aagatatatg
     4141 atttttacat tgctcagtag attgt
//
LOCUS       DAZUCE010000320         4131 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZUCE010000320 DAZUCE010000000
VERSION     DAZUCE010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.322"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            3..1544
                     /gene="mltF"
                     /locus_tag="ACHY03_004817"
     CDS             3..1544
                     /gene="mltF"
                     /locus_tag="ACHY03_004817"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFS3907940.1"
                     /translation="MNYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGELRVST
                     IHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNADLLAA
                     GLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVVVNDLQ
                     TLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELAVALDI
                     TDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVDTRTFL
                     RAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLTKNTAQ
                     SLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHMLDARAL
                     TAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQISLVGYM
                     QEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLPHSPSLLFSRKGS
                     EEKQN"
     gene            complement(1541..2044)
                     /gene="tadA"
                     /locus_tag="ACHY03_004818"
     CDS             complement(1541..2044)
                     /gene="tadA"
                     /locus_tag="ACHY03_004818"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFS3907941.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2102..2737)
                     /gene="yfhb"
                     /locus_tag="ACHY03_004819"
     CDS             complement(2102..2737)
                     /gene="yfhb"
                     /locus_tag="ACHY03_004819"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFS3907942.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2946..3794
                     /locus_tag="ACHY03_004820"
     CDS             2946..3794
                     /locus_tag="ACHY03_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFS3907943.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3850..4110
                     /gene="yfhL"
                     /locus_tag="ACHY03_004821"
     CDS             3850..4110
                     /gene="yfhL"
                     /locus_tag="ACHY03_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFS3907944.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT         1080 a         1058 c         1026 g          967 t
ORIGIN      
        1 agattaatta tctgttcatc ggcattctgg cactgctgct cgcggtcgct ctctggccat
       61 ccattccctg gtttggtaaa gccgacaacc gtatcgccgc cattcaagcg cggggagagt
      121 tgcgtgtgag caccattcat actcccctga cttataacga aatcaacggg aaaccttttg
      181 gcctggatta cgaactggcg aaacagtttg ccgattacct cggtgtaaaa ctgaaagtga
      241 ccgtgcggca gaatatcagc cagctgtttg acgacctcga taatggtaac gccgacctgc
      301 tggcggcagg acttgtctat aacagtgagc gggtaaaaaa ttatcagcct ggccctacct
      361 attattccgt gtcacaacaa ctggtttata aagtgggtca gtatcgccca cgtacgctgg
      421 gcaacctgac ggcggagcaa ctcaccgttg caccgggtca tgtggtggtt aacgatctcc
      481 agaccctgaa agaaacaaaa ttcccggaat taagctggaa ggtagacgac aaaaaaggct
      541 ctgcggaatt aatggaagat gtcatcgaag gaaaactcga ttacaccatt gctgattctg
      601 tcgccatcag cctgtttcag cgcgttcacc cggagctcgc cgtagcgctc gatatcaccg
      661 atgaacaacc ggtgacttgg tttagcccgt tagatggcga taataccctt tccgccgccc
      721 tgctcgactt cttcaacgaa atgaatgaag acggtacgct ggcacgcatt gaagagaaat
      781 acctggggca tggcgatgat tttgattacg tcgatacgcg cacattttta cgcgccgtcg
      841 atgcggtact gccgcagtta aagcccctgt ttgagaaata cgccgaagaa attgactggc
      901 gtttgctggc cgctattgct tatcaggaat cgcactggga tgcacaggcc acttcaccga
      961 cgggtgtgcg cggcatgatg atgttaacca aaaataccgc gcaaagcctc ggcattacgg
     1021 atcgtaccga tgccgaacag agcatcagcg gtggcgtgcg ttatttgcag gatatgatga
     1081 gtaaagtgcc ggaaagtgtg ccggagaacg agcggatctg gtttgccctc gctgcgtaca
     1141 atatgggcta tgcgcatatg ctggatgccc gcgccctgac ggcaaaaacc aaagggaatc
     1201 ctgacagttg ggctgacgta aaacagcgtc tgcctttact tagccagaaa ccctattaca
     1261 gcaagctgac ttacggctac gctcgtgggc atgaagccta cgcttatgtc gaaaatattc
     1321 gtaagtatca gattagcctg gtgggttata tgcaagagaa agagaagcag gctacagaag
     1381 cggcgatgca actggcgcag gattatccgg cggtatcgcc tacggagttg ggcaaagaga
     1441 aatttccttt tctctcgttt ctttcccagt cgtcatcaaa ctatttgccc cattctccct
     1501 ctctgctgtt ttccaggaaa gggagtgaag agaaacaaaa ttaatccgtc gaggattgcg
     1561 cttttttctg cgctttaatt tcctggcggc gcatgcgaaa gaagtcactg agcaacgccg
     1621 cgcactcatc cgccagtatt ccttccgtaa tttccactcg gtgattcata cccggatgat
     1681 gcagcacatc cattaaagat cccgcagcgc cagttttcgc gtcacgcgca ccaaagacca
     1741 cgcgaccaat gcgactgtgg atcatcgctc cggcacacat tacacatggt tcaagcgtga
     1801 catacaacgt ggcgtcgatc agacgataat tttgcatcac cagaccgccc tgcctcaggg
     1861 ccatgatttc tgcatgtgcg gtgggatcat gtcgaccaat aggacggttc cagccttcgc
     1921 caataacacg attgttatgc actaataccg cgccgaccgg tacttcccgc tcatcccagg
     1981 cacgcttcgc cagcgtcatt gcatgacgca tccagtattc gtggctaaat tcgacttcag
     2041 acaaaaagaa aactccggtt ataaaagcgc ggcgcattat acacggacgc tatgctttac
     2101 tctattccag ttgctggagt tcaccgcgcg gggttactcg ccaacgatgc tgacagaaat
     2161 aaagcagcgg attgtcctga ttactgtcgc tatagccact gtacagccgc agcggcgtgc
     2221 cgattttgcg ctccagttgt gcgacctttt catgtcccag acaacgcatc gtcaataccc
     2281 aaccaccata gccacgctga atttggctgg cgataagatt aactcgcggc agccagggcg
     2341 tatcgaaata aaccgcttca accagcggct gcggagagcc ggtaatcaac cagatatcag
     2401 catcagaact taacaggtag gtggttaatc gctcctgaac cagcggaaag gcggtaacat
     2461 tgtcacgaaa ccagcgtacg aaatcggcct gcaacgcctg caaacgcgct tcattgtgac
     2521 caaaagtgca tccccacaga agcagactca tcggccagcg tgccgcacga ccttttatca
     2581 ataacgcaat ggctataatc ggcaacaacg gcaggacaag taacgcattc agcggttggc
     2641 gacgcagtaa atagcgcaga aaactgccga acatatcctg ctgatgcaat gttccatcta
     2701 agtcaaaaaa caccacacga cgctcgtgag ttgccaaacc atactcctct ggactgaaac
     2761 atcctgatta attttcgcta ctacatagcc taacagatag atcatcactt ttccggcaac
     2821 aatcgaccgc atcatggcta actggaattt ttaattcatg gcaattagcg gcaatggaat
     2881 ataaaattca ctcgcgtgtg tctcatattg cctgttgtcg ccactatttc atcgcgagga
     2941 aagatatgaa cggcttactt cgtatccgtc agcgttacca ggggcttgcc cagagtgata
     3001 aaaaactggc ggattatctg ctgctacaac ctgatacggc gcgccattta agctctcagc
     3061 aactggccaa cgaagccgga gtcagtcagt ccagcgtcgt gaagttcgcg caaaaactcg
     3121 gctataaagg ttttccggcg cttaagttgg cgttgagtga agcgctggca agccagccgg
     3181 aatcgccctc cgtgcccatt cataaccaaa tccgtggtga tgatccgtta cggctggtcg
     3241 gcgaaaaact gattaaagaa aatactgccg ctatgtacgc aacgctaaac gttaatagtg
     3301 aagagaaact gcatgaatgc gtaacaatgt tgcgctctgc gcggcggata attctgaccg
     3361 gtattggcgc ttcgggtctg gtggcgcaaa actttgcctg gaagctgatg aagattggct
     3421 tcaatgctgc cgcagtgcgc gatatgcatg cgctactcgc taccgtacag gcgtcgtccc
     3481 ctgacgatct gttattagcc atttcttaca ccggtgtacg acgcgagtta aacctggcgg
     3541 cagatgagat gctgcgagtg ggcggaaaag tgctggcgat taccggcttt actcccaatg
     3601 ccctgcaaca gcgtgcttct cattgcctgt ataccattgc cgaagaacaa gcgacaaaca
     3661 gtgcttcaat ctctgcttgt cacgcgcagg gaatgttaac ggatttgctg ttcattgcgc
     3721 tgattcagca ggatctggaa ctggcaccag aacgtattcg tcatagtgaa gcgctggtga
     3781 aaaaactggt ctgagtaaag aatgcgcgta taatgcccgc ccggtttgtg ttgttttgag
     3841 agtttcctta tggcgctgtt aatcactaaa aaatgcatca attgtgatat gtgtgaaccc
     3901 gaatgcccga atgaggcgat ttcaatggga gatcatatct atgagattaa cagcgataag
     3961 tgtaccgaat gcgtagggca ctacgagaca ccaacctgcc agaaggtgtg cccgatcccc
     4021 aatactattg tgaaagatcc gcaacatgtc gagacagaag aacagttgtg ggataaattt
     4081 gtgctgatgc accacgcgga taaaatttaa tattctgctc ctgaagtaga g
//
LOCUS       DAZUCE010000321         4120 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZUCE010000321 DAZUCE010000000
VERSION     DAZUCE010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.323"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(72..1391)
                     /locus_tag="ACHY03_004822"
     CDS             complement(72..1391)
                     /locus_tag="ACHY03_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFS3907945.1"
                     /translation="MDICSRNEKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAACYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1522..3057)
                     /gene="gadC"
                     /locus_tag="ACHY03_004823"
     CDS             complement(1522..3057)
                     /gene="gadC"
                     /locus_tag="ACHY03_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFS3907946.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSKNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3213..>4120)
                     /locus_tag="ACHY03_004824"
     CDS             complement(3213..>4120)
                     /locus_tag="ACHY03_004824"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFS3907947.1"
                     /translation="WHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTF
                     GVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRV
                     KSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVI
                     AQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLK
                     EGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDY
                     KASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT         1060 a         1041 c         1054 g          965 t
ORIGIN      
        1 agtgggatag ttcaagaggg taacaagccg gtgggtaatg taccggcttg ttacaaagta
       61 agaatgggag tttaactgcc ccaacgactt tgcagatagc tgaccgcttg ttgagtctgc
      121 ggtttattca gatagtcctc acggaacaag atggtgccgc taatttctgg taccgcatcg
      181 ttaagatcga gctgcttttt cagttccggt acgccgccgt taatcatcca gtctggctct
      241 atctttgaag gttcacccac tttatagaag gcgataccga tatacaggcg ggtcctggtc
      301 ggtttaacga catccgccca ccattttgcc aacacgtcat aacacgcggc actccgtgag
      361 aacggccagt aaatttgggg agcaatgtaa tccagcaatc cttgttccac ccatcgacga
      421 gtgtcagcgt aggattcgtc ataggctgcc gcgcctcggg tatcggagcc gagcggatcg
      481 tgtgatcggt tacgccacac gcctgccggg ctaacaccaa attcgactcc cggcttaatg
      541 cttttaatgg tgtgcgatac ttttgcaatt aactgctgag tattgttgcg ccgccagtct
      601 gcttttgacg caaatgcgcc tccgtattta cggtacgttt cgttatcatt tagccgtgaa
      661 cccggtgact cagtatagaa atagtcgtca aactgcacgc catctaccgg atagcgggaa
      721 accacttctg cgactattga tgtgatccag tcctgaacct cagggatgcc cgggtcgagg
      781 acaaagcgat cgccagacgt tctgatccag tcacggtgtt gcacatagac gctcgccggt
      841 tgttgagaca gagtgctatt cagttccctg atagtaccgg gcttcgtatt aaccgatacg
      901 cgataggggt taaaccaggc gtgtactttc atcccacgct tgtgggcttc gtcgagcata
      961 aattgcagcg gatcgtaacc aggattttca ccaatcttac cggtcataag atcggaccat
     1021 ggcaaaattt ttgaaggcca cagggcggta ccgtccggct tgacctggaa aaagaccgtg
     1081 tttataccga gacgttgcaa atgatccagt ttgtcgatca tcgcctgttg ttgtacacgg
     1141 gcccggctgg tggggttact aatgttaacc gaggaaaccg gtggccagtc gaggcgagac
     1201 accgtggcca gccagatgcc acgcatcggt tgtgacgatt gttgcgtcgt ggctggtggc
     1261 tttgaacccg ctggtggtgt caccatggac tctggaggcg tgcttttaca actacacagt
     1321 aaaagtgcca gtgcaaccag tatcgctggt cttctaatcg ttaatttctc gtttcgggag
     1381 cagatatcca tatgtgctgg tttccggtaa acagatgtgc gctcattctc gtaattatcc
     1441 cggttaagtc aatacgacag taagcacgaa aaagggagcg atgaattatc gctcccttgt
     1501 cttataacca ttcagacatg gttagtgttt cttgtcattc atcacaatat agtgtggtga
     1561 acgtgcacgc gggtgcagga agaagtgacc ttttggtgcg ttcttactgt tgattggctc
     1621 cagagtgacg ccggtattcg ctttgccttt acgatcatga acagcataga gaataaaggg
     1681 cagggcaagt accaccagga aactaacgac cagtaattca acatacatat cggtagagtc
     1741 accctggatg ttatccggcg gcaggaagga aacaataaac gccataattg aagtcagcag
     1801 accgacaatt gccacgacca gtttcacccc tttaccacca gggatattaa atgtgcgttt
     1861 taagtcagga tgtttaagaa ccaacacaat gtagccaata aacagcatga aataagcaca
     1921 cagataaatc accaccgtca gcgccagtgc gatcaggaag gacatgttgt taccgccacc
     1981 ggtattggtg aggatgatca acgcgataga agtaatcacc agctgtgaaa tgaccagcgt
     2041 taccggtacg ccatttttgt tcattttagc gaatgccgct ggcagcaggt ttttctgcgc
     2101 tgtgacatac atcccgcgag aaggaccaac aatccaggag gcgatttccg ccagaacacc
     2161 cagcaacagc agtgcggaga tcacgcgaac cgtccactca atttccggtg ccacatggga
     2221 catcagaacg gtaaaggttt gcattacccc tgcggagagg ttgatttcat tacccggaat
     2281 gaccatcgca atagacaaac cgccaacaga gcttaagcag attgccgcca ccatcagcag
     2341 taacatagcc agtggatagt cgcgccctgg gttactcatt tcattgacgt gggttgcgga
     2401 agcttctacg cccatataac tcaaaatgaa ggcaacaaat acaaccaggg tgcccacttt
     2461 agagaagtca gggaagaagg tcttcgaatc catttcgata gcaacggggg caccggagtg
     2521 cagataaata gccgctaatg caatcaaaat aaatgcaggt aacaggatac cggcgaagaa
     2581 gccaacttta gcaattcgcg ccgtgtattt cgtgccacca aactgcgtta atgccagcgc
     2641 ccaaagaatg atgagtgctg caatagtttt ggtaatgggg tcttcattca gcgctggcca
     2701 tttcaggatg taggagagtg cccctaacac gaaatagagc atcggaataa aaccaatggc
     2761 gatttgcaga tagccaaatg agatcgctgc aaatccccat ctcggcccca gagtatttga
     2821 tacccaggcg aagacaccac cttcttccca gccgtcgacg gtggccattt ccgcagcaca
     2881 aagtcccacg ggaataaacc ataaaatccc gcctaatagc aggaagaaga ctaatgaaaa
     2941 gcccgatgtt gcgaaggtag ggtattcata aacagccatt accatcgatg ccgttatggc
     3001 aaaaaagccg agtaatgtga gctgcttagc tttacctgtc tgtactgatg tagccatatt
     3061 atccccctaa aacggtattc ctgtcggaac cgcacctgtt ttgttctgaa gcgtattcag
     3121 aacaatattt tccgttgcta atgccagtga acagactttg gaaattgtcc cgaaacgggt
     3181 tcgtttcggg acaccgttac cgttaaacgt tatcaggtat gtttaaagct gttctgttgg
     3241 gcaataccct gcagtttcgg gtgatcactg agatatttca gggaggcttt gtagtcttcc
     3301 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
     3361 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
     3421 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac cctctttcag tttgaagcaa
     3481 accgccggga tgccttcgtc cgggcgcccc gtacagatga actcatacgg ccccagtttg
     3541 gcgatttcat ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtatag
     3601 ccttcacgac cgaggcgcag gaattcatag tactgtgcaa ttacctgacc cgccgggcgg
     3661 gagaagttga tggcgaaagt accaatctga ccaccgaggt agtcaacgtt gaacaccagt
     3721 tcctgcggca gcgcttcttc atcacgccag ataacccagc cgcagcccag cggagccaga
     3781 ccgaatttat ggcctgaagc actgatcgat ttcacacgcg gcaggcggaa gtcccagacg
     3841 atatccgggg cgacgaacgg tgccaggaag ccaccgctgg cggcgtcgat gtgcatgtcg
     3901 atgtcgatac cggtgtcggc ctggaattta tccagcgcat cgtgcagcgg ttgcgggaac
     3961 tcatagttac cggtgtaggt cacgccgaaa gtcggcacca cgccgatggt attttcgtcg
     4021 caggcttcaa tcatgcgttt cggatccata aacaactgac cggggcgcat agggatctca
     4081 cgcagctcca catcccagta gcgggcgaat ttatgccagc
//
LOCUS       DAZUCE010000322         4053 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZUCE010000322 DAZUCE010000000
VERSION     DAZUCE010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.324"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            201..452
                     /gene="rem"
                     /locus_tag="ACHY03_004825"
     CDS             201..452
                     /gene="rem"
                     /locus_tag="ACHY03_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFS3907948.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            519..797
                     /locus_tag="ACHY03_004826"
     CDS             519..797
                     /locus_tag="ACHY03_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907949.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            799..1848
                     /locus_tag="ACHY03_004827"
     CDS             799..1848
                     /locus_tag="ACHY03_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFS3907950.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGDVGALSDWLLRHVTSCQWPNGDYHH
                     TETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKSPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEDKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            1862..2614
                     /locus_tag="ACHY03_004828"
     CDS             1862..2614
                     /locus_tag="ACHY03_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFS3907951.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEASTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(2892..2981)
                     /locus_tag="ACHY03_004829"
     CDS             complement(2892..2981)
                     /locus_tag="ACHY03_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907952.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(3036..3248)
                     /gene="cspF"
                     /locus_tag="ACHY03_004830"
     CDS             complement(3036..3248)
                     /gene="cspF"
                     /locus_tag="ACHY03_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFS3907953.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            3549..3764
                     /gene="cspB"
                     /locus_tag="ACHY03_004831"
     CDS             3549..3764
                     /gene="cspB"
                     /locus_tag="ACHY03_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFS3907954.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
     gene            complement(3754..>4053)
                     /locus_tag="ACHY03_004832"
     CDS             complement(3754..>4053)
                     /locus_tag="ACHY03_004832"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907955.1"
                     /translation="AGLRLWCFLWESSTYALCFAMPAVSFCCKTIHAANLHFTTMVSI
                     LFLIKILPNMLAIVSCIGPPYFLLKRSNIHYSVRISTQASAFFVIVYRRAMNFNQ"
BASE COUNT         1115 a          850 c         1018 g         1070 t
ORIGIN      
        1 gaaatccctc gccacctctg atgtggcagg catcctcaac gcacccgcac ttaacccgct
       61 tcggcgggtt tttgttttta ttttcaacgc gtttgaagtt ctggacggtg ccggaataga
      121 atcaaaaata cttaagtagc gcgcagggat aagagggatg gtcccttaaa ggggagagct
      181 aattatccgg aaggattctg atgatgaaca tcgaagaact gcgtaaaatt ttttgtgaag
      241 atggcctcta tgctgtgtgc gttgaaaatg gaaatcttgt tagtcattac cgcattatgt
      301 gtttgcgaaa gaatggggct gcgttaatta attttgtgga tgctcgggtc acggacggat
      361 ttatcttgcg cgaaggtgag tttgtcactt cattacaggc attgaaagag atcggaataa
      421 aagctggctt ttctgctttt tcaggagaat aaactcatct acaatcttgc gcggggctga
      481 actcccgctg agtaacaccg tgccaccgga gaaaaccgat ggcacgcaac gcaaaatatt
      541 acaattctga taattcgccc gttcttgcct gcacgcacgg gcggtattct cacgcattca
      601 agtctgaatg gttccagcac cctccatgca ctgcagaaca ggccgaatgg ctgattcatt
      661 cttaccgcag gcgcgggttc gaggttaaga aagctctcag tctcgactat cggcactgga
      721 taatctctgt caggctgcct tattccgaac gcccaccacg tgcgtcccgc actttccagc
      781 aacggatctg gaggtaacgt gcgggtatta cttagacctg ttctggtgcc tgagcttggg
      841 ctggtggtcc ttaagccggg ccgtgaatcc atacagatat ttcataatcc tcgagtgctg
      901 gtggaaccgg aaccaaaaag catgcgtaat ctgccatccg gagtcgttcc tgccgttcgc
      961 cagccgctgg cggaagacaa aacattgctg ccgtttttta gtaacgaacg ggtgattcgt
     1021 gctgctggcg acgttggcgc attgtccgac tggctattac gtcatgttac atcctgccag
     1081 tggcctaatg gcgattacca tcacactgaa acagtcattc accgttatgg taccggcgca
     1141 atggtgttgt gctggcactg cgacaaccaa ctgcgtgacc agacatcgga atcactggag
     1201 ctgcttgctc aacaaaatct gacagcatgg gtgattgacg tcatccgtca cgcaataagc
     1261 ggtacgcagg agcgggaatt atctttggct gaattatcct ggtgggcggt ctgcaatcag
     1321 gtggtggatg cactacctga ggctgtatcg cgtcgttcgc tgggattacc agcggaaaaa
     1381 atctgctcgg tgtaccgcga aagcgacatc gtaccgggag agcagaccgc caccagcata
     1441 ttgaaacaac gcacaaaaaa tcttgcaccg ttgccttacg cccaccagca acaaaaatca
     1501 ccacaggaaa agacggtggt aagcatcacc gttgatccag agtctccgga atctttcatg
     1561 aagctgccta aacgtcgccg ctgggttaag gagaaataca cacgttgggt taagacacag
     1621 ccgtgtgctt gctgcggtat gccagccgac gatccgcatc atctgattgg tcacgggcag
     1681 ggcggaatgg gaacaaaagc acatgatctc tttgtgttgc ctttgtgcag aaagcatcac
     1741 aacgagctgc atacggatac agtggcattt gaagataagt atggctccca actggagctg
     1801 atatttcgtt ttatcgatcg cgcgctggca attggcgtac tggcgtaagt ggagaacgag
     1861 catgaacctt gaagccttac caaaatatta ctccccaaaa tctccaaaat tgagcgatga
     1921 cgctccagcg acaggcaccg gttgtttaac aattacggat gtaatggcag cgcaggggat
     1981 ggtgcagtcg aaagcaccac ttgggttggc cttatttctg gcaaaagttg gtgttcagga
     2041 ccctcagttt gcgattgaag gcctgctaaa ttacgcgatg gcactggata acccgacatt
     2101 gaacaaattg agtgaagaaa tccggttaca gattattcct tacctcgtga gttttgcctt
     2161 tgctgattac tccaggtctg cggcaagtaa ggctcgctgt gagcattgtt caggtacggg
     2221 attttataat gtattgcgcg aagtggtgaa acactacaga cgcggggaat ctgtaatcaa
     2281 ggaagaatgg gtgaaggaac tatgtcagca ttgccatggt aagggcgaag ccagcacagc
     2341 gtgcagaggg tgtaagggta aagggattgt tctggatgaa aaaagaaccc ggtttcatgg
     2401 cgtaccggta tataagattt gtgggcgttg taatggaaac cggtttagtc gtttaccgac
     2461 cacgctggca cgacgtcatg tccagaagct ggtaccagac ctgaccgatt atcagtggta
     2521 taaggggtat gcggacgtca ttggtaaact ggtaacaaag tgctggcagg aagaagcata
     2581 cgcggaagcg caattgagga aggtgacgag ataaatgatt tttgctgaag atggcgacat
     2641 gatgtttgca tttttcaaaa aatatggata aaattttttc aacgatgggc tttgtatacc
     2701 cgacgttaag aaaaagtaga aaacccgctg atgagcgggt tttgtgcttt aaatggggca
     2761 atggtaatgt tgaatctcat cccgggactc atgtctgtta acttattatt tagctggtga
     2821 cttggttatt tgcctgatgt ttaaaatgtt ttcttccagt acaatgtccc taaacacaat
     2881 gagtctgctt attatattat tagcagagct attacggcca aagtacagca taagctttta
     2941 aagccaatca accagtcatc aagacagacg gggttattca taaaaactct ccatgtgtga
     3001 tccgatgggg cctgaaatta aagctttaat atagctcatg aaaggtaaac attggcagct
     3061 gaagggccac gcagaccatt tatccggcaa aattccacgc gtaatccggt ggtaatttct
     3121 tctgcatcgc ggagattgag cgctgaaaca tgaagctgga catcgatacg accatcggat
     3181 ggggtgataa gacccttgcc gcttttgccg tcaaaggttt tgacaattcc tgtcatttta
     3241 cgggacaaaa aaattcctta atactgataa cttggcgcac tatacacacg ttcctgaaga
     3301 aagctatagt tttttgatgg ggttgaagat ggctggatgt ctaaaataaa cattgcttca
     3361 tatgttcaac tatgcgttaa tgattgcgtc ggtttgaaga acagacgata tacgaagtag
     3421 tttactaaag cagttctcat ttcaggtgtt attcacttat tccttctttg agtctctcca
     3481 attaagtacg aagtcgtttc tgttatgcaa accatttatg ccgaaaggct caagttaagg
     3541 aatgtagaat gtcaaataaa atgactggtt tagtaaaatg gtttaacgct gataaaggtt
     3601 tcggctttat ttctcctgtt gatggtagta aagatgtgtt tgtgcatttt tctgcgattc
     3661 agaatgataa ttatcgaacc ttatttgaag gtcaaaaggt taccttctct atagagagtg
     3721 gtgctaaagg tcctgcagca gcaaatgtca tcattactga ttaaaattca tcgctcgtct
     3781 gtatacgata acgaagaagg ctgatgcctg agtagagata cggacagagt agtgaatatt
     3841 ggatctcttt aataaaaagt aaggaggtcc aatacatgaa acaatggcta gcatatttgg
     3901 caaaatctta atcaggaaaa gtatgctaac cattgtggtg aagtgcaggt ttgctgcatg
     3961 aatagtttta cagcagaagc taactgctgg catggcaaaa caaagtgcgt aagtggatga
     4021 ctcccacaaa aagcaccaca atctcaaacc cgc
//
LOCUS       DAZUCE010000323         4035 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZUCE010000323 DAZUCE010000000
VERSION     DAZUCE010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.325"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..77)
                     /locus_tag="ACHY03_004833"
     CDS             complement(<1..77)
                     /locus_tag="ACHY03_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3907956.1"
                     /translation="MTETESAILAHARRCAPAESCGFVV"
     gene            complement(83..781)
                     /locus_tag="ACHY03_004834"
     CDS             complement(83..781)
                     /locus_tag="ACHY03_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS3907957.1"
                     /translation="MQDIQQETLNECIRAEQSASVVLWEIDLTEVGGDRYFFCNEQNE
                     KGESVTWQSRQYQAYPIQGSGFEMNGKGASARPTLKVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSEITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(781..1119)
                     /locus_tag="ACHY03_004835"
     CDS             complement(781..1119)
                     /locus_tag="ACHY03_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3907958.1"
                     /translation="MANTEEFHWLPEDGMKTENKPSIKTVRFGDGYEQRSPNGLNHSL
                     RVFTCDFKVEANERDSFEKFLARHEGYKSFFWRPPGINRKIRVVCRTWSATEHITYTD
                     FSCQFDEVVI"
     gene            complement(1112..>4035)
                     /locus_tag="ACHY03_004836"
     CDS             complement(1112..>4035)
                     /locus_tag="ACHY03_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS3907959.1"
                     /translation="TEELSGVAQAQREAEKAGKLAAAQQEAQAQAFQRMLDKIDPLAA
                     ALRNLEQQQDELNAAFASGKINGSQFENYSRKIQETRRELTGEAQAEREAAKAHDEQV
                     AALQRLIAQLDPVGTAFNRLVEQQKQLNEAKAKGMLSPEMYEELSGKLRAMRSELEVT
                     QSQLSKTGMSAKQTAFAMRMLPAQMTDIVVGLSTGQSPFMVLMQQGGQLKDMFGGIGP
                     AIKGVGSYVLGLINPFTLAAAAVGVLGLAYYKGSQEQDEFNKSLILTGNQLGTTSGQL
                     ADIAQRAGNAADSTTGAAAAVLNQLVRSGKVASSSLEQVTTAIVKTSEVTGISTEQLV
                     NDFNEIAKDPVSAISKLNDQYHFLTIATYNQIKALQDEGNQQEAARIATEAYSSSMIQ
                     RTNQIKENLGYLETAWKAVADSAKWAWDSMLDIGREASLDQKISDVLRQIDEIEKNTR
                     PGVFGLGGIGDGGAQNKRLARLKQQLGVLQAEKIAQDVLNSSINDYNKRQQEGIELRQ
                     RADAFSKQYQTREQQRASELAKLEKLKSQYSKEEYNNLIAQINERYKDPKQPKAKGYS
                     DDAAQRMIDHLNQQNALLSSQTELTVKLSSSEQELVKWRQQIADLESRPSSKLTKDQK
                     SLLLHREEITALMEKNVAIEKNNRLIKESAEIAAWRDSLQASIDNRQQGYDIQIAGYG
                     VGDKNQQRQQELLRIEREYNNQRLQLERDYADKSRGMSNHVFQEKMQALNDALEREKE
                     IVRQKNEQLDIQAGDWVSGASQGFNNWLDDTKDISEQIKSTTTQMFDGMTDALGDFVT
                     TGKANFRSFATSVISDLSRIALKASITRIFDSISNSSSGGILGTIGSAISKFIPNAKG
                     GVYESPSLNTYSNGIYDSPQFFAFAKGAGVFGEAGPEAIMPLTRTSDGSLGVRAINSK
                     SGNGGGDITYAPVYQITIQNDGQNGEIGPQAIKALMGMVDQRVQGNLLNMRRDGGMLS
                     G"
BASE COUNT          951 a         1075 c          828 g         1181 t
ORIGIN      
        1 ctcaccacga agccgcacga ctccgctggc gcacatcgcc gggcgtgcgc cagaatcgct
       61 gattctgtct ctgtcatggg atttactgcg aaagtttgtt aatggaaagg aagccgccaa
      121 agttgccgac attattgcgg aacttacagc cactcaggca tttgctgcat ttatccttcg
      181 tgatttcgga tgtcggctgg tcatattcat ccgcgaccgc cggaccgtga taaccgcact
      241 catcaccgcg ataggtccag gtgcaggtat tagcaagcat gatgcgcccc ggaaaaacag
      301 cgccgtccgt ttccgtcggc gtggacagta caaaggaggc actgaccgcg ctcagttcgc
      361 tgcactgctc aatacgccag cggctgatca cctcctgctc cggatcggcg tcactgtttc
      421 cgttgacgaa gttcaccgca tccagaaaac gggcgtaaac cttacgcctg accaccgttc
      481 cgccgaccag actctgcagg tcttccgcca tcccggtgac catgccgtgc agattagaga
      541 ctttcagcgt tggccttgca ctggcacctt tgccgttcat ctcaaatcca ctcccctgaa
      601 tgggataggc ctgatactgc cgactctgcc aggtgacaga ctctcctttc tcgttctgct
      661 cattgcagaa aaagtagcga tccccgccta cctcggtcag gtcgatttcc cagagcacca
      721 cgctggccga ctgctccgca cgaatgcatt cattcagcgt ttcctgttgt atgtcctgca
      781 tcagatcacc acttcgtcaa actgacacga aaaatcggta taggtgatat gttctgtcgc
      841 tgaccacgtt cgacacacca ctctgatttt tctgttaata ccaggcgggc gccaaaaaaa
      901 agatttatag ccttcatgcc gggctaaaaa tttttcaaat gaatcacgtt cattcgcctc
      961 aactttgaaa tcacaggtga aaacgcgcag agaatgatta agtccatttg gacttcgttg
     1021 ctcataacca tcgccaaatc ttacagtttt tatcgatggt ttattttctg ttttcattcc
     1081 atcctctggt aaccagtgaa attcttccgt attagccact taacattccc ccatcacgtc
     1141 gcatatttaa cagattgccc tgcacccgct gatcaaccat ccccataagt gcttttattg
     1201 cctgagggcc aatctctcca ttctggccgt cattttgaat agtgatttgg tatacagggg
     1261 cataggtaat atctccgcct ccattaccac ttttactatt aatagctctg acaccaagag
     1321 aaccatcgga agttcgtgtt aatggcataa tggcttccgg accagcctca ccaaaaacac
     1381 cagccccttt tgcaaaagca aaaaattgcg gggaatcata aataccgttc gaatacgtgt
     1441 tcaatgacgg agactcataa acaccgccct ttgcattcgg aataaattta ctaatagcac
     1501 tcccgatagt tcctaaaatc ccccccgaag aactgttact aatgctgtcg aaaatccgag
     1561 taattgaagc ctttaatgct attcgactaa gatccgaaat cacggaagta gcgaaagaac
     1621 gaaaatttgc cttgcctgtc gtgacaaaat cacccagcgc atcggtcatt ccatcaaaca
     1681 tctgagtcgt ggttgatttt atctgctcac tgatatcctt agtgtcatcc agccagttat
     1741 tgaatccctg ggaggcacca ctaacccagt ctcctgcctg aatatcgagc tgctcgtttt
     1801 tctgtctgac aatttctttt tctcgttcca gagcatcatt cagagcctgc attttctcct
     1861 gaaaaacatg atttgacatc ccacgggatt tatctgcata gtcacgttca agttgcagac
     1921 gctgattgtt atattcacgt tcaatccgca gtaattcctg ctggcgttgc tgatttttat
     1981 cgccaacccc ataaccagca atctgaatat catacccctg ttgacgatta tcaatcgaag
     2041 cctgcaatga atcacgccat gcagctattt cggcggattc cttgattagc ctgttatttt
     2101 tttcaatcgc aacatttttc tccatcaacg cggttatttc ttcccggtgt aagagaagcg
     2161 atttctgatc cttggttaat ttcgatgacg gtcgtgactc caggtcggca atctgctgac
     2221 gccacttaac cagttcctgt tcagaggaac ttaatttaac ggttaattca gtttgtgaac
     2281 ttagtaacgc attctgttga ttcagatgat caatcattcg ttgggcagca tcatccgaat
     2341 aacctttcgc ctttggttgc tttggatctt tataacgctc atttatttga gcgataagat
     2401 tattatattc ttcctttgaa tactgactct ttagtttttc cagttttgca agttcactag
     2461 ctctctgctg ctcccgggtc tgatattgtt ttgaaaaggc atctgctctc tgtctgagtt
     2521 caattccttc ctgttgtcgc ttgttgtaat cgtttattga tgaatttagt acgtcctgag
     2581 caattttttc tgcttgaagt acgcccaatt gctgctttaa tcgtgccagc cttttatttt
     2641 gagctccgcc atctccaatg ccacctaacc cgaaaactcc gggtcgggta tttttttcta
     2701 tttcatcaat ttgacggaga acatctgaga ttttttgatc aagggaggcc tcacggccaa
     2761 tatccagcat ggaatcccat gcccattttg cggagtctgc gacagctttc catgcagtct
     2821 caagataacc aagattttct ttaatctggt tggtgcgctg gatcattgag gatgagtatg
     2881 cttctgtcgc aatgcgggcg gcctcctgct ggttcccttc atcctgtagc gccttaatct
     2941 ggttataagt cgcaattgtc agaaaatggt actgatcatt aagttttgat atggcactga
     3001 caggatcctt tgcaatttca ttgaagtcat taaccagttg ttcggttgat attcctgtta
     3061 cttcgctcgt ttttactatc gctgtcgtca cttgctccag cgaactgctc gctacctttc
     3121 ccgaacgcac aagctggttt aatactgccg ccgcagcgcc agttgtcgaa tcagccgcat
     3181 tcccggcacg ttgagctata tcggccaatt gcccgctggt tgtccccaac tgatttccgg
     3241 taagaataag agatttatta aattcgtcct gctcctgaga gcctttatag taagccagcc
     3301 ctaagacgcc aacggctgct gcggccaggg taaaaggatt aattaatccc agcacataag
     3361 aaccaacacc tttgatcgcc gggccaatcc caccgaacat atcttttagc tggccgccct
     3421 gctgcattaa caccataaaa ggcgactgac cagtggacaa cccaacaaca atatccgtca
     3481 tttgtgcagg caacatgcgc atagcaaaag ccgtttgttt tgccgacatt ccggttttgc
     3541 ttaattgaga ttgagtaacc tcaagctcac tccgcatagc acgaagtttt ccagaaagtt
     3601 cctcatacat ttcaggagag agcatcccct tagcttttgc ttcattgagc tgtttctgtt
     3661 gttctaccag acgattaaaa gcagttccga cagggtcaag ttgagcaatc agacgttgca
     3721 aagcagcaac ctgttcatca tgcgcttttg ctgcttctcg ctctgcctga gcctctccgg
     3781 taagctctcg ccgtgtttcc tgtatttttc ggctataatt ctcaaactga gaaccattta
     3841 ttttcccgga tgcaaacgca gcattaagtt catcctgctg ttgttcaaga tttcttagcg
     3901 ccgcagccag agggtcgatc ttgtccagca ttctttgaaa ggcctgagcc tgcgcttcct
     3961 gctgggcggc agcaagtttt ccggccttct cggcttctct ctgcgcttgc gcaaccccgc
     4021 tcaattcctc tgtgg
//
LOCUS       DAZUCE010000324         4024 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZUCE010000324 DAZUCE010000000
VERSION     DAZUCE010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.326"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            102..554
                     /locus_tag="ACHY03_004837"
     CDS             102..554
                     /locus_tag="ACHY03_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFS3907960.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            707..1981
                     /locus_tag="ACHY03_004838"
     CDS             707..1981
                     /locus_tag="ACHY03_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFS3907961.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            1974..>4024
                     /gene="mchF"
                     /locus_tag="ACHY03_004839"
     CDS             1974..>4024
                     /gene="mchF"
                     /locus_tag="ACHY03_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFS3907962.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKL
                     TRMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECAKASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     A"
BASE COUNT         1148 a          755 c         1009 g         1112 t
ORIGIN      
        1 tgatatgaaa aattttatgc ttaagttttc cagagatgca tttggatatc ttgaaccgat
       61 aagaaacaca gtgtaaaaaa tatataaggg aaatagtaat catgtcttat ataagggaaa
      121 ccatcagagg aaaagatgaa tggactgttt atgaacagat cggttttgcg gtcagttgta
      181 tgctctacaa tcgtaattac agtctgtatc cggtgttaac cattcaatac tggactgaat
      241 atgcgataca gcataatcag attaaattcc tgtttgattc acgaggtttt ccactggcgt
      301 atattacctg ggcatatctt gaggctgata cagaagcgcg cctgctcagg gatccagaat
      361 tcaggttgca tccgtctgaa tggaatgaag atggaaggat ttggattctg gatttctgtt
      421 gtaaaccagg ctttggtcga aaagttattg actatctcat acagcttcag ccatggggag
      481 aaggagaagt acgatggtta agcaggcgaa agaaaattgt gacatacatc cctgagcggc
      541 tgcataaaac gtagtacctc tgaagataca gagataattg taaattacgg ggtaaatgca
      601 tcgctgatac tattttgaca ggactctgta ttttctggct ttgattatag catttcgctt
      661 aaccagtgaa ggtattacag gaaaagtgtg agtaagagga gctattttgt ttcgtcagga
      721 tgctttagaa aacagaaaaa tgaagtggca gggacgggca atattacttc ccggaatacc
      781 actatggtta atcatgctgg gaagcatagt gtttattacg gcatttctga tgttcattat
      841 tgttggtacc tatagccgcc gtgttaatgt cagtggtgag gtcacaacct ggccaagagc
      901 tgtcaatata tattcaggtg tacagggatt tgttgtcagg cagtttgttc atgaaggaca
      961 gttgataaaa aaaggggacc ctgtttatct gattgacatc agtaaaagta cacgtagtgg
     1021 tattgtcact gataatcatc gtcgggatat agaaaaccag ctggttcgtg tggacaacat
     1081 tatttcccgt ctggaagaaa gtaaaaaaat aacgctagat accctggaaa aacaacgcct
     1141 gcaatacaca gatgcgtttc gtcgctcatc agatattata cagcgtgcag aggaagggat
     1201 aaaaataatg aaaaacaata tggagaatta cagaaactat caggcaaaag ggctgattaa
     1261 taaagatcag ttaactaacc aggtggcatt atattatcag caacaaaaca atcttctcag
     1321 cctgagcgga cagaacgaac agaatgccct gcagataacc actctggaga gtcagattca
     1381 gactcaggct gcagattttg ataaccgtat ctaccagatg gaactgcaac ggtacgagtt
     1441 acagaaagaa ctggttaaca ctgatgtgga gggcgaaatc attatccggg cgttgactga
     1501 cgggaaagtt gactccctga gtgtcactgt cgggcaaatg gtcaataccg gagacagcct
     1561 tctgcaggtt attcctgaga acattgaaaa ctattatctt attctctggg tcccgaatga
     1621 tgctgttcct tatatttcgg ctggtgacaa agtgaatatt cgttatgaag cctttccggc
     1681 agaaaaattt gggcagttct ctgctacggt taaaactata tccaggactc ctgcgtcaac
     1741 acaggaaatg ttgacctata agggagcacc acagaatacg ccgggcgact ctgttccctg
     1801 gtataaagtc attgcgatgc ctgaaaagca gattatcaga tatgacgaaa aatacctccc
     1861 tctggaaaat ggaatgaaag ccgaaagtac actatttctg gaaaaaaggc gtatttacca
     1921 gtggatgctt tctcctttct atgacatgaa acacagtgca acaggaccgc tcaatgacta
     1981 acgggaattt cagacaaatt ataaatcagc ttgatatgcg ctggcgacgt cgtgttccgg
     2041 ttattcatca gacggagacc gctgaatgtg gactggcctg cctggcaatg atatgcggtc
     2101 attttggtaa gaatattgac ctgatatctc ttcgccggaa gtttaatctc tcggcccgtg
     2161 gagcaaacct tgcaggaatc aatggaatag cggagcagct ggggatggtc acccgggctc
     2221 tttcactgga gctggatgaa cttggtgccc tcaaaatgcc gtgtattctc cactgggatt
     2281 tcagtcactt cgtcgtgctg gtcagcgtaa agcgtaaccg ttatgtactg catgatccgg
     2341 ccagaggcag aagatatctc ggtcgggagg aaatgagccg gtattttacg ggcattgcac
     2401 ttgaggtctg gcccggaagt gaattcctgg cggaaaccca gcagacccgc ataagtctcc
     2461 gttcactgat taacagtatt tacggtatta aaagaacact ggcgaaaatt ttctgtctgt
     2521 cagttgtaat tgaagcaatc aatctggtaa tgccggtggg aactcagctg gttatggatc
     2581 atgcgattcc ggcgggggac agagggctgc tgacgcttat ttctgctggc ctgatgttct
     2641 ttatattgct cagggccgcg gtgagtatgc tgcgtgcatg gtcctcactg gttatgagca
     2701 cgctcatcaa tatacagtgg cagtcgggtc tgtttaacca tcttctcaga ctgccgctgg
     2761 cgttttttga acgccgtaaa ttaggtgata tccagtcgcg ttttggctcc cttgacactt
     2821 tgagggccac ctttaccacc tgtgttgttg gggcaatcat ggacagtatt atggttgtgg
     2881 gtgtttttgt gatgatgctg ttatatggag gatatcttac ctggatagtg ctcggtttta
     2941 ccatggttta cgttcttatt cgtctggtga catacggcta ttaccggcaa atatcggaag
     3001 aaactcttgt caggggggcc cgggccagct cctattttat ggaaagcctg tatggtattg
     3061 ccacggtaaa aatccagggt atggctggga ttcggggaac acactggctt aacctgaaaa
     3121 tagatgcgat caattcaggt attaagttaa ccaggatgga tttgctcttc ggggggataa
     3181 atacttttgt tgccgcctgt gatcaggtgg cgattttatg gctgggtgca agccttgtga
     3241 tcgataatca gatgacaata ggaatgtttg tggcatttgg ttcttttcgt gggcagtttt
     3301 cggatcgggt tgcttcgctg accagttttc ttcttcaact gagaataatg agtctgcata
     3361 atgagcgcat tgcagatatt gcactacatg aaaaggaaga aaagaaaccg gaaattgaaa
     3421 tcgttgctga catgagcccg gtttcactgg aaaccactga tttaagctac cggtatgaca
     3481 gccagtcagc acaggtattc agtggtctga atttgtctgt ggctccggga gaaagtgtgg
     3541 ctataactgg tgcctccggt gccggaaaaa ccacattaat gaaagtatta tgtggactgt
     3601 ttgaaccaga tagtggaaaa gtactggtta atggcacgga tatacgtcaa cttggaataa
     3661 ataattatca ccgtatgata gcctgtgtta tgcaggacga ccgactattt tcaggatcaa
     3721 ttcgtgaaaa tatctgtggg tttgcagaag aaacagacga cgaatggatg acagaatgtg
     3781 ccaaagcaag tcatattcat gatgtgataa tgaaaatgcc aatggggtat gaaacgttaa
     3841 taggtgaact gggggaaggt ctttccggcg gtcaaaaaca gcgtatattc attgcccgag
     3901 ctttataccg gaaacctgga atattattta tggatgaggc tacaagttct cttgatacag
     3961 aaagtgaacg tttcgtgaat gctgctataa aaaagatgaa tatcacccgg gtgattattg
     4021 caca
//
LOCUS       DAZUCE010000325         4021 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZUCE010000325 DAZUCE010000000
VERSION     DAZUCE010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.327"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..314
                     /locus_tag="ACHY03_004840"
     CDS             <1..314
                     /locus_tag="ACHY03_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907963.1"
                     /translation="KIDVAVRLVGLKAYDLHEGIPDRPKRKVKDGINEDIKADTSLRL
                     SKTNISDTSLNRYRRTVKEIINNEIDVFLLEQKKKNKRFPYSEDRDVIRPLWGKCLTG
                     Q"
     gene            complement(401..997)
                     /locus_tag="ACHY03_004841"
     CDS             complement(401..997)
                     /locus_tag="ACHY03_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907964.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDIRHKVVATLELQRRSQLEGVFVRKFWEEQDVAQIEGIVVDGAVRDVSL
                     ATFVYETISTKAGVILLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGD
                     RICYDGESIPESEIWSEHPDRNKHCVVLVAESVNGKAA"
     gene            complement(1308..1383)
                     /locus_tag="ACHY03_004842"
     tRNA            complement(1308..1383)
                     /locus_tag="ACHY03_004842"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1348..1350),aa:Thr,seq:cgt)
     gene            complement(1498..2751)
                     /gene="proA"
                     /locus_tag="ACHY03_004843"
     CDS             complement(1498..2751)
                     /gene="proA"
                     /locus_tag="ACHY03_004843"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFS3907965.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2763..3866)
                     /gene="proB"
                     /locus_tag="ACHY03_004844"
     CDS             complement(2763..3866)
                     /gene="proB"
                     /locus_tag="ACHY03_004844"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFS3907966.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         1018 a         1071 c          952 g          980 t
ORIGIN      
        1 agaagattga cgttgccgta cgacttgtag ggctgaaagc ctatgatctg catgaaggga
       61 ttccggatag gccaaaacga aaagttaaag atggcataaa tgaagacatc aaagccgata
      121 ccagtttacg cctgtcaaag acaaatataa gtgatacgtc gctaaaccgt tatcgccgca
      181 ctgttaagga gattatcaat aacgaaatcg acgtattcct gttggaacag aagaaaaaaa
      241 ataaaagatt tccctacagt gaagatagag atgtgattcg acctttatgg ggaaaatgcc
      301 tgaccggaca gtaactccct gaggggataa cagcccggtt atcccctgtt cgttatgcct
      361 cggtataccg gagttccagg gacacgagac aatcggcaaa ttatgcggct tttccattaa
      421 cagattcggc cacaagaaca acacagtgtt tgtttcgatc aggatgttca ctccagattt
      481 ctgattcagg aatactttcc ccatcatagc agatccgatc accatcaaac ggataatacc
      541 gtgcggaatc tgtatctaag ataaacacct taagattcgt tgaacgacgc gctatatgct
      601 gccagagggc tttacctcct tcatattgct cgttatcact taatagtata acgcctgctt
      661 ttgtggatat tgtttcataa acaaacgtag caaggctaac atcacgtaca gcgccatcga
      721 caacaatccc ttcaatctga gcaacgtcct gttcttccca gaatttccgg acaaacaccc
      781 cttcaagctg tgaacgacgc tgaagctcca gagtggcaac aaccttatgg cgaatatcac
      841 catcaataac agaaggtgca gcttcgatta ccgcagcgaa cggagtcttt ggatcccaga
      901 tgatctggta aagcacaccc tgaatagtta ctccatccag tatcgaaaaa cgacgagcta
      961 cagtagcagg ggagtaagac attggaatga cagccatttt atggcctaaa atttgaaaac
     1021 attagtatca atactgacct catcagtctg agcatatatt gataaagttt ttatgtttac
     1081 actcaataaa taccttgcca aacaactaat agcgatgtct acattgccat tcgacatgct
     1141 tacaattttt gtcaacaaga aagtgaattt atatcaacgg taagaagtaa acacgaggac
     1201 accaaaacca aatgtacaca cttttgtgct ttgtgtcccc tgtgtgtacc caaatgacaa
     1261 actgcaagaa aatctaggca cacacttatc taacttattg atttaaatgg tgccgataat
     1321 aggagtcgaa cctacgacct tcgcattacg aatgcgctgc tctaccaact gagctatatc
     1381 ggccctgaaa ggacatgttc acgaacgtga atcacggtgg acaaggttaa aactaaccgg
     1441 gcgatgcgtc aatggccttg tgaatcaaat ggctactttt gcatcacccg gttttattta
     1501 cgcacgaatg gtgtaatcac caatgccgat ccacttgtaa gtggtcagcg cttccagccc
     1561 cattgggcca cgcgcgtgga gtttttgtgt gcttaccgcc acttccgcac ccagtccaaa
     1621 ctggccgccg tcggtaaaac gcgtagaggc gttaacgtaa acagcggacg aatccacttc
     1681 gttaacaaaa cgctgggcgt tgcgcatatc gcgggtcagg atcgcatcgg agtgttgtgt
     1741 gccgtgttca cgaatatggg cgatggcatc gtcaagatcg ctgacgattt tgacgttcaa
     1801 atctaatgac agaaactcat cgtcatactc ttcggcttta accgccacca ccttcgcggg
     1861 gcctgcctgc aactgtgtca gcgctgctgc atctgcgtgt aatgtcacgc cgctttccgc
     1921 catttgtttg cttaatgcgg gcaggaagct atcggcgatg tttttattca ccagcaacgt
     1981 ttctaccgta ttacatgtgc tcggacgctg agttttcgcg ttgacgatca cttttaatgc
     2041 ttcagcgatc tctgcacttt catcaacgta aatatggcat acgcctatac cacctgtgat
     2101 caccgggatc gtcgactgtt cgcggcacag tttatgcaaa ccagccccgc cgcgcgggat
     2161 cagcatgtcg atgtatttat ccatacgcag catttcactg accagcgcac ggtcaggatt
     2221 atcaatcgcc tgcacggcac ccgccggtaa gccacaggat ttcagggcgt cctgaatcac
     2281 cgccaccgtt gccgcgttag tgcgacaggt ttctttgcca ccacgcagga tcaccgcatt
     2341 accggttttc aggcacagtg aagcaacatc aaccgtcacg ttcgggcgcg cttcataaat
     2401 cacgccaata acccccagcg gtacgcgacg acgctcaaga cgcaggccgc tgtccagtac
     2461 gccgccatcg attacctgcc ccaccggatc ggcgaggtta cacacctggc gcacatcgtc
     2521 ggcaatgcct ttcagccgtg cgggcgtcag tgccagacgg tcaagcatcg cttcgctaag
     2581 gccattggct cgcgcgtcag caacatcctg ggcgttagcg ttgaggatga tttcgctttg
     2641 tgcttccagt tcatcggcga ttttttccag cacgcgattt ttttcgcggc tggagagttg
     2701 cgctaattta tacgaggctt gcttcgcggc aatgcccatt tgttccagca tcggccagct
     2761 ccttaacggg taatcatgtc atcacggtga acggcaaccg ggccgtattc atatcccagt
     2821 attgcatcaa tttcttgcga gtggtgtccg gcaatacggc gtaatgcatc gctgttgtaa
     2881 cgactgacgc cgtgggcgat atcgcgacct tcgaggttac aaatgcggat gacttcacca
     2941 cgcgagaagt tgccagtcac gcttttaatg cctttcggca acagggagct gccgcgttca
     3001 agaatggcgg cggttgcccc ttcatctacc gtgatttcac ccgccggcgg cgcaccgaaa
     3061 atccagcgtt tacggttttc aagcggagtc gcctgggcat ggaacagcgt accgacggaa
     3121 atgccttcca tcacatcacc aatgacgccc ggcttgctgc ccgcggcaat aatggtgtcg
     3181 atacccgcac ggcaagccac gtccgcggcc tgcaatttgg tactcatgcc gccagttccg
     3241 aggcctgaaa cgctgtcacc ggcaatcgcg cgcagtgcgt catcaatacc gtaaacatct
     3301 ttaatcaatt ctgcctgcgg attactgcgc gggtcagcgg tatacaaacc tttttgatcg
     3361 gtcagtaaca acagtttatc ggcacccgcc agaatcgccg ccagtgcaga aaggttgtcg
     3421 ttatcgccga ctttaatctc tgccgtagcg acagcatcgt tctcattgat taccggaacg
     3481 atattgttat cgagcaacgc acgcagggtg tcgcgggcgt tcaggaagcg ttcacggtct
     3541 tccatatcag cacgggtcag cagcatttgc ccgacgtgaa tgccataaat cgaaaacagc
     3601 tgttcccaca gttgaatcag tcgactctgc cctaccgccg ccagcagttg tttcgaggcg
     3661 atagtcgctg gcagttccgg gtaacccagg tgctcacgtc cggcggcgat cgcgcccgac
     3721 gtcacaataa caatccgatg cccggcggca tgtaactgcg cgcactggcg aacaagttca
     3781 acgatatggg cacggttcag gcggcgcgat ccgcctgtta gcacactggt gccgagtttt
     3841 accaccagcg tctggctgtc actcatgatt ctctgccatt caattttagg aaaaatgata
     3901 tcaaacgaac gttttagcag gactgtcgtc ggttgccaac catctgcgag caaagcatgg
     3961 cgttttgttg cgcgggatca gcaagcctag cggcagttgt ttacgctttt attacagatt
     4021 t
//
LOCUS       DAZUCE010000326         3921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZUCE010000326 DAZUCE010000000
VERSION     DAZUCE010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.328"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(2..1681)
                     /gene="bcsG"
                     /locus_tag="ACHY03_004845"
     CDS             complement(2..1681)
                     /gene="bcsG"
                     /locus_tag="ACHY03_004845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFS3907967.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDFTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1678..1869)
                     /gene="bcsF"
                     /locus_tag="ACHY03_004846"
     CDS             complement(1678..1869)
                     /gene="bcsF"
                     /locus_tag="ACHY03_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFS3907968.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1866..3437)
                     /gene="bcsE"
                     /locus_tag="ACHY03_004847"
     CDS             complement(1866..3437)
                     /gene="bcsE"
                     /locus_tag="ACHY03_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFS3907969.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3710..3898
                     /gene="bcsR"
                     /locus_tag="ACHY03_004848"
     CDS             3710..3898
                     /gene="bcsR"
                     /locus_tag="ACHY03_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFS3907970.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         1028 a          962 c          980 g          951 t
ORIGIN      
        1 cttactgcgg gtaaggcacc cagtcgccgc cgttcaggcg aacgtacggt ttatcctggt
       61 attgaataac tactgcattt gagttctcgg agaccggtgc tgtttgtggc aacccactgg
      121 tgagtttttt ccagtcaaca ttgtcttcgg tgaaaatctt gccatcaaga acgcgaacca
      181 ccagatcgga gatagccagg aagctgctcg gttggtcgat gacaatcggt gccccctgat
      241 gtggtgcctt catgccgaag aatttcaccc caacggggac gtcggtgata gacgggctag
      301 ggatatcacg taggccagat acctgcattc tgtcgccctt cagcgcgccg ccgtgttccg
      361 gcaccacgac caccatcact ttacgacccg atttctccag ttcagtaaag aaggcgtcca
      421 gttcatcaaa gaatttctgc gcccgcgctt tgtaatccgc tgttttgctg acgcccggat
      481 aatggttgcc gtcatgcagt ggaagcgtgt tgtagaacgt ggcactacgg ctgtttttat
      541 ctttttcggt aaagtccagc cagcggttaa gcacggcggt atcgtcataa accggcgaac
      601 catcaaagcc cagcaaaata accggcagat ttgtttgatc catcaattcg ctctgcatgc
      661 cgccattttc gcgaacttct ttcaaaaaac cgccgaactg gccgttatgt cccatcatca
      721 ggtgctgggt aaagcccagt ttcgaaaggt tatcaaacag atagcagtcg ttatttgccg
      781 gttgatacag attagtgtgc gaagtctgcc cgcagctggc gcgcagtaaa cggatcgccg
      841 ccgggccact gtaggaggtg gcggagttaa agttcttgaa ctcaatatcg aaatgcgacc
      901 acagtggatg cgacatcaac ccagcggctt ctatatccga ccaggaaagc gaacagatgt
      961 taatcaccag tagttcaaat ggctgagcat cagcgggcag cgaagacggg aaggtcgatt
     1021 tacgtttcgc ctccgcgtta tagaaattat tcagccaggc gttaaggttc gccgttgttg
     1081 gcggtgcagt ttgtgcgggc atatcaccca ctaccggtgc gccacccgtc gccgcaacgg
     1141 ttgccgctgc gttaccaccc gtcgttgtta cagtggtcgt cggttgtccg gctggccaca
     1201 aggagaaact tggtcccgcc agggtaagta cgttcagcca tagcagtatg gcaaccacaa
     1261 aaacggtaat gcgaatccat tgtgacagga ataaccaggc cactaataaa acaaaaatgg
     1321 ccccaatcat ctgccagtta ataaagcgtg tgacaaggtc gattaaataa tcggtactga
     1381 accccgccac ctgcgaaccc tggctcatta tgctttccgg gccaggcaac caggtgtcat
     1441 gccagaacaa agcaaagccg atcggcaggg caatccagtg gcgcaagcga tgcaggctgt
     1501 agcgcggaat gggcatcagc agaaacgcgg caaacaccaa attgaggagc ggatggaagt
     1561 taagatatcc cgcccacaac aggccgaact taaccagaaa atagaagttc cagccggaaa
     1621 ggccgcgcca gtattgccag agggaagaag gcatggcggt attttgcgta aattgagtca
     1681 ttttttggtt gccctggctt tttccgtgcg gcgtaacgtc ccagccggtt taacataacg
     1741 aggtttagca aagaacaaac gtaaggtgtc gcgaatgcgt cgcaaagagt gccgcgccag
     1801 atagcccagc gggaaaaata tcagtgcgca aacgacaata atttcaatga tatcgctgat
     1861 ggtcatcatg atgagcgctc cacagcatca tctaacagtc gcatgggttc tggtattcgt
     1921 cgccagtggc gaccatcgtg ctcggcattg atgaccggtt tagaactttg tgttaaaggc
     1981 agcggcatgc cccattgttc tggggcaagc aggcgcatct gcaccagctc ggcactgatt
     2041 tgatcatctt caaaccagac catacggttt gagaaaatgt cgccagtagg caatgggaaa
     2101 atatgattca acgcggtatc cagatcgtta atccgacaga atgagagaaa cagcaccagc
     2161 cgattaccgc caatggtcat gatatcgccg gtacggttag ggcgacacag cgtcagggct
     2221 tgttcaacgc ggatacccgg taccggacgt agggcaacca gaacgccttt accgtgggca
     2281 ggtaatagag ggttattcat catgttgttg acggcattac agaacacatc ccacttctgg
     2341 aaaccacgca gtttgagcgg ctgggtcatt gacagcaagg tagtgatatc ttccggcaca
     2401 tagcgactaa acttctgccc ttgcacgctt tcgatcatcg tcagacaacg ggagagtggc
     2461 gcattccacg gaataaccat atttgcaccg caggccaata acaaacgttc atcggtggcg
     2521 cgcaggctag cggtattttc ccgtacgagg attttcatcg cactaccgcg ctggcgacgc
     2581 agagtatgaa tgctgcgggc cagtggctcg atttgcgcat tttgctgtaa agaaaagacc
     2641 accgtcgccg cctgagcggt acgggcttca ttgaacagga cttcgttatt gttgaacagt
     2701 tgccagtgtt ccgatagcgg cggcgcacct tccagtacag caacattact cagaatgcgt
     2761 ttttcgtcgc tgcgtggttg gatctccgcc tcttcgcttt gaactaatgt ccagatacca
     2821 ttttgttgct gaacgctaag ctgctgacgg gcgctgaccc ctttttcgtt gcaccagaag
     2881 gcaatatcca gcaaatgttg gtcgccctga aaacgcaaac tggcaagacc aaaaagtgaa
     2941 cggtattcct caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc
     3001 aaaagcgaac aatggtttaa cctgctccat ttattcattt tatccaacca tgagcgcagc
     3061 cgctcggcag gaatgttttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta
     3121 tggggatcaa tagaacactg caaatcacgg gccaaatagt atagaccttt ttcatgattt
     3181 agcattgaaa ataattttat tttttccggc ccttgagaat catctaattg aaagattttc
     3241 gccggatcgc tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt
     3301 gtttgattcg ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg
     3361 cctgctggca tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg
     3421 tccacaatgt ccctcatgtc accattttgt ctgattatca gtttactagc gaaagctaag
     3481 aaataaacct accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagcag
     3541 gtcacaagac gtgtgatttt agtcttttcg tttttaactt tttctgttgc gtaacggaaa
     3601 gtcaaaaagt gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg
     3661 ttagaattgt ctcatgaacg gtacggttat ttcataggga tcaagcaaaa tgaataacaa
     3721 tgaaccagat actctgcctg atcccgcgat aggctatatc ttccagaatg atattttggc
     3781 gttaaagcag gcattttcac tgcctgatat tgattatgcc gatatttccc aacgcgaaca
     3841 gttggccgcg gcattaaaac gctggccgtt gctggcagag tttgcgcaac aaaagtaggg
     3901 gattggtgaa tggccgtact g
//
LOCUS       DAZUCE010000327         3897 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZUCE010000327 DAZUCE010000000
VERSION     DAZUCE010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.329"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..225)
                     /locus_tag="ACHY03_004849"
     CDS             complement(<1..225)
                     /locus_tag="ACHY03_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFS3907971.1"
                     /translation="MSRVAAIIYALVICIIVCLSWAVNHYRDNATAYKEQRDKNAREL
                     KLANAAITDMQMRQRDVAALDAKYTKELADA"
     gene            complement(222..755)
                     /locus_tag="ACHY03_004850"
     CDS             complement(222..755)
                     /locus_tag="ACHY03_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS3907972.1"
                     /translation="MNAKIRYGLSAAVLALIAVGAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIEQ"
     gene            complement(819..1169)
                     /locus_tag="ACHY03_004851"
     CDS             complement(819..1169)
                     /locus_tag="ACHY03_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFS3907973.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARMSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            complement(1174..1389)
                     /locus_tag="ACHY03_004852"
     CDS             complement(1174..1389)
                     /locus_tag="ACHY03_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFS3907974.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
     gene            complement(1682..1756)
                     /locus_tag="ACHY03_004853"
     tRNA            complement(1682..1756)
                     /locus_tag="ACHY03_004853"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1721..1723),aa:Gly,seq:tcc)
     gene            complement(1760..1835)
                     /locus_tag="ACHY03_004854"
     tRNA            complement(1760..1835)
                     /locus_tag="ACHY03_004854"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1800..1802),aa:Thr,seq:tgt)
     gene            complement(2201..2738)
                     /locus_tag="ACHY03_004855"
                     /pseudo
     CDS             complement(2201..2738)
                     /locus_tag="ACHY03_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
     gene            complement(2735..3025)
                     /locus_tag="ACHY03_004856"
     CDS             complement(2735..3025)
                     /locus_tag="ACHY03_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFS3907975.1"
                     /translation="MVDLRKAARGQMCAVRIPGYCNHDPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(3025..3624)
                     /locus_tag="ACHY03_004857"
     CDS             complement(3025..3624)
                     /locus_tag="ACHY03_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFS3907976.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSHGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT         1013 a         1024 c          826 g         1034 t
ORIGIN      
        1 cgcatcagct aactccttcg tgtattttgc atcgagcgca gcaacatcac gctgacgcat
       61 ctgcatgtca gtaattgccg cgttcgccag cttcagttct ctggcatttt tgtcgcgctg
      121 ctctttgtag gcggtggcgt tatcacggta atgattgacc gcccacgaca ggcagacgat
      181 gatgcagata accaaagcat aaataatcgc ggcgactctg ctcactgctc tatcccccaa
      241 caggctaatg cgctttcctg gtcacgacga ataacctgtc catagcagtt atttgaacgt
      301 atgcggcaat cgcgcccacc atcttttatc caccagcgaa tcgcctcgca tgcgccctta
      361 cgatcaccgg cattcagccg cttataaaac gtcgacggga aacacttacc ggggccaatg
      421 ttatagggac agaatgacgc tatacccgct ttttgtggct cggtcagtgg tactttaata
      481 ttgcgctcca cccatgccag cgccttatca cgctcaatgg cgttgacctg gtcgcatttt
      541 tccttcgaca gtttcatacc gggaaaaacg ggttttccat ccaccatcgt ggcaccccga
      601 cagatggtcc agatgccgga cccatcgcgg tatgccgttg tgtggttacc ttctttttca
      661 tccagaaact ggtcgagaat atcaggcgcg ggcgcaccga cggcaatcag tgccagaacg
      721 gcagccgaca ggccgtatct gatttttgcg ttcatggata tttatcagga tttatcggtt
      781 tctgcccacg gacaggttta tctgttccgg tcagtgactt aaggttgtga ttccggagga
      841 gtcttcagag aaccagtaat tcttcccggt agctttcctt tgtaggttat ccatacattc
      901 tgcgcctcta aaattacggg gcgcttttcc ggcgactgct catccccttc acataacccg
      961 gcagcaacat ccaggaagac ctgtctgatg ctcattctgg ctgctgcctc ataaaactcc
     1021 agcgcggcac cttcaacacg gtccagcgag atgtccaggt caaaaatttc accgtcaaag
     1081 cgttttttgt cccgtaacgc taaagttacc gtaactttat tctcaaaatt gcggatccct
     1141 ttcacaatca gttcatagtt ttgagtcatt gaattactct ccccgtgcag ccttacgctt
     1201 gtcttctctg attttgaagt acagatttgt cagataagtc aggaagccca gaaccagact
     1261 ccccagcaca ccaatcgcag cccactgtga cggactgacc tgatcaagcc actgtaaaaa
     1321 ccagtagcca gcactgcctg cggaggtgcc ataggcaatg cccgttgaaa ttttgtccat
     1381 ggatttcata gcctcacctc cgcaaataac ggatggcgta gttttacact gagaaatgaa
     1441 agggatttga aaagaaaagc ccgcaaaagc gggcgaaaca atatatacag taaggaaagc
     1501 actctatcca acaaaccacc cacagttaat cggaataaaa gcagagtgct tatgaatgat
     1561 cgcctgcccg aaggttagta tttctgcaca gcaattttgc taaaaaaagc gatcattcat
     1621 aacttaaacg tctttcagtc actccgggat ttcccatcat cgcagactga aagactctaa
     1681 ctggagcggg cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc
     1741 attatacgat acccgcatat ggtgccgact accggaatcg aactggtgac ctactgatta
     1801 caagtcagtt gctctaccta ctgagctaag tcggcattgg ttcttcaggg gagcgatatc
     1861 accgagcgat atcaccgagc aaagaagagt tccccctcag aaccgttttc gatgatacaa
     1921 tttaatattc caatcgcaac aacactttgc gtcaagctat gtaaatttat ttatgtattt
     1981 ttattttatg tgaacatttc accttcactt aaaatatacg cgacaatata taaacaaatt
     2041 tattttgaag gcaatattaa atgtcgtttc ttatatcaca ccacaataac aacaaaaccc
     2101 gcttgatggc gggttctatt aaagtttaat tgcgcttgat tcgcctcgcg atacagcttt
     2161 gcgaagcgta gcaaaattga agcagtttat gcgtaaaaaa tcaagccgtt ttttgagcga
     2221 atgattctcg catgggaatg tatagcgcat actcagcaac ggccaaccaa ttagcaattc
     2281 gcttttcgca tgtgctaaaa caccactctg ggtgtgcatc atttagcaat tcagccattt
     2341 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
     2401 gctctgccag cacctcactt atgactcgat caatacataa cgcctctgca tcagtacaat
     2461 gcgccagcca gctcttttgc ttgccgttga tcatctctcg caaaaacgct tccagctcag
     2521 ctttctctat tcccgctttt ttcattctgc gcagggcttc attgatggct gttttcgtca
     2581 attttttgga tgccaacaac tgattgaaca tatttcctga cctgccaccg ccaatatacg
     2641 accagcgccc tacgcagttt gccctgaatc cagacacttt ccagcgtggc gagacgaagg
     2701 tgttctccgc tttttcctgt attcgttggg taaatcacaa atatccctcc tttctccaga
     2761 tttcttgtgt gcgaaaaaca ccttctgcat gcatcaggcg taattcttct ttggtgtaat
     2821 cgctggtttt tacccgcccg tcgattagat cgtggcatga gctacaggca atcgccgcct
     2881 gcatatcgtg tggttttgtc gctgttccgc acgttcccgc cagtcggtaa tgcgccagca
     2941 cagacgtttc cggatcgtga ttgcagtagc caggaattct gacggcgcac atctgccccc
     3001 gcgccgcttt acgtaaatcc accattacgc aaactccagc agctgcgcgg ccacattttc
     3061 gacttgttcc ggagaggaaa atttacggaa cagaatccag ttccacagca cattcagaac
     3121 agatttataa acctgctgaa actcggtttc atccatattc gcaaaagcga tggatttcgc
     3181 ccgacgccca tggctaccgt ccggataaaa atgttcggtg taaaatccag cctgaatggt
     3241 tacccactcg cgaaaagcct caaacgactt tagcaatgcc gtatcccggg ttctgcgtgt
     3301 cgcaactgta ttcagatatt gctctgcggc ctcactcagg gctggcgtgt gttcccgacc
     3361 aactgattcg cacaggtaat caacgaaacc agacagcagt tctcgttcgc gtggcgtgat
     3421 cgccccaccg accggagtcc agtaatcgaa tcccagttgc aggagtttga aaaaacgctt
     3481 gtggaatgcg tagttacgca cacgcttaaa gtctgcgtgt atccactcgc ctattttgat
     3541 ttgatgcaga aaatcacaac tctccggcgt cgccgggaga agtaatccgg aagaggtttg
     3601 tttgaccagt tgtatatgcg ccatttctta atctctcgat ggcgcagcgc agcagatgcc
     3661 agttgttcag gctgacgtat gaagtataaa taaactggct ccagtgtaaa gccccacctt
     3721 aatggaataa aaaccaaaca acagattgct gggatacaaa caacgcttat tattaaaagc
     3781 ggttaaacaa attaaatttt aatgttatgc aaatctgcca gatcaccata atatctcatt
     3841 tgaaaaccgc tgaaataaca accctatcag ggttaatcat attaaggtga gtaaata
//
LOCUS       DAZUCE010000328         3886 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZUCE010000328 DAZUCE010000000
VERSION     DAZUCE010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.330"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..237
                     /locus_tag="ACHY03_004858"
     CDS             <1..237
                     /locus_tag="ACHY03_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS3907977.1"
                     /translation="DAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAADLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(450..1307)
                     /gene="sitD"
                     /locus_tag="ACHY03_004859"
     CDS             complement(450..1307)
                     /gene="sitD"
                     /locus_tag="ACHY03_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFS3907978.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILRLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGVTIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRCLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLAIGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNT
                     HTQDLSSPN"
     gene            complement(1304..2161)
                     /gene="sitC"
                     /locus_tag="ACHY03_004860"
     CDS             complement(1304..2161)
                     /gene="sitC"
                     /locus_tag="ACHY03_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFS3907979.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2158..2985)
                     /gene="sitB"
                     /locus_tag="ACHY03_004861"
     CDS             complement(2158..2985)
                     /gene="sitB"
                     /locus_tag="ACHY03_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFS3907980.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDEHPFVAHRPSAVQREAR"
     gene            complement(2985..>3886)
                     /gene="sitA"
                     /locus_tag="ACHY03_004862"
     CDS             complement(2985..>3886)
                     /gene="sitA"
                     /locus_tag="ACHY03_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFS3907981.1"
                     /translation="KVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGD
                     AAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVI
                     VSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADT
                     YKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTP
                     QQVRKVVDMVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPT
                     YIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1038 a          999 c          952 g          897 t
ORIGIN      
        1 gatgcagaag cagaaaaact gttccggatc cgggaggcgg aagaaacaaa aaacagcctg
       61 atgcaggtag ccagtgagca tattgcgccg cttcaggatg ctgcagatct ggaaattgca
      121 acggaggaag aaacctcatt gctggaagcc tggaaaaagt atcgggtgtt gctgaaccgt
      181 gttgatacat caactgcacc tgatattgag tggcctacga accctgtcag ggagtaatca
      241 ttgggattat gccgcagaaa cgttgtatgc aataacgttc tgcggttggc tgatgcactt
      301 tcgagagtgc atgtattgaa ttatttccag ctattatcga ttttacgtat tttttgcatg
      361 agagaatttg tacctcctcc caacaacgac cttccatgac tttccggact tctgtgggcc
      421 agcggacgat gctgtcctcg gatgttgtgc taattgggtg atgacaaatc ctgtgtatgg
      481 gtgtttccct ttgtgcgtgc gttgatgctg gtaacggtga acgtcatgat gaacacaacc
      541 gcgaataaga cgacaatcgt cggagctggt gcactgtcga taaaaaagga caaccagaca
      601 ccagtcattg acactattac cgatatgcca atcgccagta ggagcgcaat atgaaagcgt
      661 tgtgtcagga gtaccgcaat ggcgccaggc gcaatgagca gagaaatgga gagaatgata
      721 cctaccgctt tcagcgtcgc cacaatggtc agagagacca tgcacagcag cccataatgc
      781 agacatctcg tatgcaaacc gcttacctgc gcctgttgat aatcaaagct gaacagcaaa
      841 aaatcccgcc attttacgct aataacaagt gtaaccagcc cggcaataat caccgtctgg
      901 attatatcgc cgatggttac cccgagcatg tcgccgaaaa gaatatggtc aagatgtacg
      961 tctggtttga ctgcaatata caagatcaga cctgcggcaa acatgccaga aaaaacaatc
     1021 cccatcactg tatcttgctt gattcggctg ttgtctttca ggtatcccgt cgccaccgcg
     1081 cagaatactc cggcgacgaa tgcccctgta gccagaggta acctcagaat ccaggcaagc
     1141 acaataccag gaaaaaccgc atgactcatc gcgtcaccca tcagtgccca acctttcagg
     1201 accagaaaga ccgacagcag cgcacagggt atggaaacta ccagtgaaat cagcagtgcg
     1261 tgactcataa aggtgaactg cagtggctcc agtaataatg ccatcatgag cgatcctcca
     1321 gcgctttatg cgcacgtcga cgatttgcca aaagcccatg ggtgggcgca aagacaaatg
     1381 ctaaaagaaa cagcagtgtt tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa
     1441 agtaactgac ccacgcgccg aggaaactgg ttacactgcc aatagttaca gcaattatga
     1501 gcaggcgggg gaaacgatcg gttaacaacc atgctgttgc accgggagtc accaccagac
     1561 agatcaccag aaaggcacca accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg
     1621 tgaagaagag gatttttaat ctgcccggat gcaatccaat agcacgggcg tgattctcgt
     1681 cgaaaaaggt caccataaga tctttccatt tgaaaaaaag cacgatgatc gagagaatgc
     1741 caatgatagt gagttgcagg atatccgccg gatcgattgc cagaatattg cccaggacga
     1801 tggtctgaat gttcacggat gtcgggttca atgacaccat aaacagcccc aggccaaaaa
     1861 aggaggagaa gatcaggccg atgatggcat cttcttttaa gcgagtgcgt tggttaagaa
     1921 acagcatgct gcccgctgcc agtccgccgg aaaagaaggc tcccagagaa aacggaagcc
     1981 ccagcatata tgcccccgca acacccggga caatagagtg cgacagcgca tcgccaatca
     2041 gcgaccagcc ttttagcatc agatagcatg acaggaaagc gcagagaccg ccgaccatcg
     2101 ccgagaccca catcgcattg agcatatatt catagctgaa gggttccagt agcacgttca
     2161 tcttgcttcc ctctgcaccg ctgacggtcg atgtgccaca aaagggtgtt catcgtcagt
     2221 aataatgctt tcttccgagc cgttaagtgt gacatggcgc agtacgccgc taaaagccag
     2281 ctccaggttc tcggctgtaa aagtggtgtc tgtcggcccg cttgccagga cggtgccttt
     2341 gaccatgacc gtataatcgc aaaatgtcgt gactgagccg agattgtgcg ttgagacaag
     2401 catcgtcttg ccttccgcgc gtaactccct caacaggctg atgattttcg cttcggtttt
     2461 cacatcaacg ccggtaaaag gttcatccag caggatcaca tcaccctgct gtgcaatcgc
     2521 tctcgccaga aagacgcgtt tcttttgtcc accggaaagc tcgccgattt gtcgatggcg
     2581 aaaatccacc atatcgacac gttccagcgc atcggtgaca atctgacgat ccctcttttt
     2641 ggctatacgc aacataccca tgtgcccata gcggcccatc atcaccacat cttccactag
     2701 tacaggaaat gaccagtcaa cctcttctga ctgagggacg taggcaacca ggtttttctg
     2761 tagcgcctgt cgcgtgggaa tacccagaac agatatcttt ccgctggtca gacgcacaaa
     2821 tcccataatc gctttaaaca acgtcgattt tccggaaccg tttaccccaa ccagagcggc
     2881 aatagatcca cttggtactg tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca
     2941 ggtgacagta acatcattaa cgacaatgcc cgcagattgc atcattattt ctcccttttc
     3001 ccggctttaa ttccctgtac cagcgtactg gtagtaacct tcagaaggtc gatatacgta
     3061 ggtaccgggc cgttttctgt gctcagggaa tcgacataga gtacaccgcc gtagtgcgcg
     3121 ccggtttcac gcgcaacctg acgcgctggt ttatcggaaa tcgtactctc gctaaagact
     3181 gccgggatat gatttttctt caccatatca acaaccttac gtacctgctg cggcgtgcct
     3241 tgttgatcgg cattaatcgg ccacagataa agctctttta gccccaaatc gcgtgcgaga
     3301 taagaaaaag ccccttcact ggtgaccatc catcgctgat tctcagggag ttcagcaatc
     3361 tgcttacgca ggggggcaag ggtttgggta atcttggctt tataagtatc ggcattacgt
     3421 tgataggttt gtgcatttgc cggatcgtat tttatcaacg catcacgaat attatcgacg
     3481 taaatcagag cattatctgg cgacatccag gcatgggggt taggtttgcc ctcatagggg
     3541 ccttcagtga tccctactgg cgtcacaccc gaagagacaa ttacttctgg aaccccatta
     3601 agatgctggt aaaagcgttg gaaccacaat tccagattca taccattggc gagaatcagt
     3661 tgtgccccct gcgcacgttt aatatcgcca ggggtaggct gatactcatg aatttctgca
     3721 ccgggcttgg ttatggatga gacttctgca gcatctccag ccacgttttt ggccatatct
     3781 gcgatgatgg taaatgttgt aatgaccttg aatttttcag ttgcactcgc ctgaaatgcg
     3841 atcgagcagg tgagggcgag gcaccccaag agcatggtta cttttt
//
LOCUS       DAZUCE010000329         3875 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZUCE010000329 DAZUCE010000000
VERSION     DAZUCE010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.331"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            21..1679
                     /gene="dpiB"
                     /locus_tag="ACHY03_004863"
     CDS             21..1679
                     /gene="dpiB"
                     /locus_tag="ACHY03_004863"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFS3907982.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1648..2328
                     /gene="dpiA"
                     /locus_tag="ACHY03_004864"
                     /gene_synonym="citB"
     CDS             1648..2328
                     /gene="dpiA"
                     /locus_tag="ACHY03_004864"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFS3907983.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2368..3753)
                     /gene="dcuC"
                     /locus_tag="ACHY03_004865"
     CDS             complement(2368..3753)
                     /gene="dcuC"
                     /locus_tag="ACHY03_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFS3907984.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1051 a          938 c         1004 g          882 t
ORIGIN      
        1 gacaacccgc aaggaaaaca atgttgcagc ttaacgagaa taaacagttt gcatttttcc
       61 aaagactggc atttccgctg cgtatctttt tgctgattct ggtgttctca atatttgtca
      121 ttgcagccct ggcgcaatat tttacggcca gttttgagga ctatttaacg cttcatgtac
      181 gcgacatggc aatgaatcag gcgaaaatta ttgcctccaa tgacagtgtc atctctgcgg
      241 tgaaaacgcg tgactacaaa cggctggcga ccatcgctaa caaattacaa agagataccg
      301 attttgatta tgtggtgatt ggcgaccggc actcgatccg cctttaccat cctaatccgg
      361 agaaaattgg ttatcctatg cagttcacca aacagggcgc gctggagaaa ggggagagct
      421 acttcattac cgggaaaggg tcaatgggta tggcgatgcg cgccaaaacg ccaatctttg
      481 atgacgatgg aaaagtcatc ggcgtggtgt cgattggcta cctggtgagt aaaatcgata
      541 gctggcgggc tgagttttta ttaccgatgg caggtgtgtt tgtcgtgctg ttagggattc
      601 tgatgttgct gtcgtggttc ctggccgcgc atatccgtcg gcagatgatg ggcatggagc
      661 caaagcaaat cgcacgcgtg gtccgtcagc aagaggcgct gtttagttcg gtttatgaag
      721 ggctgattgc ggtggatccg cacggttaca ttaccgccat caatcgtaac gcaagaaaga
      781 tgctggggct gagttccccc ggacggcaat ggttgggtaa acccattgct gaagtggtca
      841 ggcccgccga tttctttacc gaacagattg atgaaaaacg tcaggatgtg gtagctaact
      901 ttaacggtct gagcgttatt gccaatcggg aagctattcg ttcaggtgat gatttgctgg
      961 gggccattat cagctttcgt agtaaagacg aaatttccac cctcaatgcg caactgacgc
     1021 aaataaaaca atacgtcgag agcctccgta cattgcgaca cgagcatctc aattggatgt
     1081 cgacgctcaa tggtctgttg cagatgaaag agtatgatcg cgtgctggcg atggtgcagg
     1141 gggagtctca ggcccagcaa cagcttattg acagtctgcg cgaggcgttt gccgatcgcc
     1201 aggtggcggg gctgcttttt ggtaaagtgc agcgcgcccg cgaactgggg ctaaaaatga
     1261 tcatcgtccc cggtagccag ctttcgcaac tgccgccagg actggatagc accgagtttg
     1321 ccgccattgt tggcaattta cttgataacg ccttcgaagc cagcctgcgt agcgatgaag
     1381 gaaacaagat cgttgaatta ttcctcagcg atgaaggcga tgatgtggtg attgaagtcg
     1441 ccgatcaggg ctgcggcgtt ccagagtctc tacgagacaa aatatttgag cagggggtca
     1501 gtacgcgtgc tgacgagccc ggtgaacatg gcattgggtt gtacttgatt gccagctacg
     1561 taacgcgctg cggtggtgtt atcactctcg aagataatga tccctgcggt accttatttt
     1621 caatctatat tccgaaagtg aaacctaatg acagctccat taaccctatt gatcgttgag
     1681 gacgaaacgc cgctggcaga gatgcatgcg gaatatattc gtcacattcc cggattcagt
     1741 cagatattac tggcgggaaa cctggcgcag gcccgaatga tgatcgagcg ttttaagccg
     1801 gggctaatct tgctcgataa ctatcttcct gacggtagag ggattaattt actgcatgaa
     1861 ctggtgcagg cgcattatcc cggcgacgtg gtgtttacta ctgcagccag cgatatggaa
     1921 acggtgtctg aagccgtacg ttgtggtgta tttgattatc tcattaagcc cattgcctat
     1981 gaacggctgg ggcaaacgct aacccgtttc cgccagcgta aacatatgct ggaaagtatt
     2041 gatagcgcca gccagaagca aattgatgag atgtttaatg cctatgctcg cggtgaaccc
     2101 aaagacgaac tgccgaccgg cattgacccc ttgacgctaa acgcggtgcg aaaattgttc
     2161 aaagagcctg gtgtgcaaca tacggcagaa acggtggcgc aggcactgac catcagccgc
     2221 accactgcca ggcgttatct tgaatattgc gccagccgcc atctgattat tgctgaaatt
     2281 gttcacggca aagttggcag accgcaacgc atataccaca gtgggtgaca tgataaagcc
     2341 ggaggaaact gcctccggca tgactattta cttgcctgtg accgctgctg ccgttcctgg
     2401 caccatcagc tctgtagcaa cgataacaat caccagccca acaagcaccg gcaccgaggt
     2461 gcgttttacg acttcaaatg gtgagatttt cgccatcccg gcaaccgcaa cgactacgcc
     2521 agaaacgggt gacagggtac gaccgaggtt tgacgcctgc agcatcggga tagtcaaata
     2581 cgccgggtta atgccggaag agtgcgccag tttcgggatc atctcaacaa acgcataaaa
     2641 cggcgcattg cctgaaccgg tcgtgactgc cgccagcatt gtcagaatca ccaataccag
     2701 catcaggatg atactcgccg aaccaaacga ggtagcgata gaaatcagac tttgaataaa
     2761 gccgatggtg ctaagcccct gagcgaatac cccagcggca accagcagca tcaccacgtt
     2821 agcaaacgca tctgccatcc cgcgataagc cacttccaga ccagagaaaa ctttctgggt
     2881 attaaagctg cggaggaact ccagaatgga ggcaatcagc atacaaatca ccagaatagt
     2941 gatgatgtgt aattgcggac cccatttacc gtcaaaaatc agtacaccga tgatcggcgt
     3001 gaacggcaaa atggcataaa acgcaggagc agtggtggtg atttcactga catctaacat
     3061 ttcatgagag atgtgctctt ttttatccag ataacgttgc cagaagaagt gggcgatcgc
     3121 catgccgata attgcagcaa ttgagatagg cagcgtcgtt ttgaaggcga agtcaatcag
     3181 cgacatttcg gaagcttgcg ccgccagcac cacatcccct gaagtcggtg cgagaataat
     3241 cgccgccggg gaggcacaaa tggcagctgc tgccccacga ctgataccaa cgtttaccat
     3301 caccggaaac agggttgcca tcagcaaaac acccagaccg gttgcggaag agacggccag
     3361 tgacatcaga caggcaacaa aataggcggc aatcatcagc aggtaggggg agttaatata
     3421 ctgcaatggt tttgacgcca gcttgaccac catatcattc gcgccgatat gggtcatgta
     3481 agcggcaaag ccacacagca tcataatcat catgccgagg tcgccgccgc ggctcattag
     3541 caatatttta acgtattcaa cgatatccgt ggcgctgtag cctgttgaag cctggctgga
     3601 cggtaacact ttgtgcccca taatggcact aataatcagc aataacaggc caccgacaaa
     3661 taacacgcca gtggcggaat accctttaat gatgtagcga gctacaccca caataaccac
     3721 aaccccaata aggagttcaa tgaatgtcag cataattttt cctgtctcca ggccccaaag
     3781 taaataataa aaaaatccta aagcttaagg aaaaaatatg cccaataaat tggcggtgaa
     3841 tgctgattaa aatcaagaaa aactgccatt aagac
//
LOCUS       DAZUCE010000330         3874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZUCE010000330 DAZUCE010000000
VERSION     DAZUCE010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.332"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            65..781
                     /gene="nanC"
                     /locus_tag="ACHY03_004866"
     CDS             65..781
                     /gene="nanC"
                     /locus_tag="ACHY03_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFS3907985.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWGSMESNTWNTIHENKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            801..1907
                     /gene="nanM"
                     /locus_tag="ACHY03_004867"
     CDS             801..1907
                     /gene="nanM"
                     /locus_tag="ACHY03_004867"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFS3907986.1"
                     /translation="MNKTITALAIMMASFATNASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1971..2951
                     /locus_tag="ACHY03_004868"
     CDS             1971..2951
                     /locus_tag="ACHY03_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFS3907987.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLHFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYSSHPQHFNHMIEAFRRDLIQYHSQLNKIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSSAARRGIISDRFVEAILQFWREK"
     gene            3074..3769
                     /locus_tag="ACHY03_004869"
                     /pseudo
     CDS             3074..3769
                     /locus_tag="ACHY03_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1078 a          888 c          936 g          972 t
ORIGIN      
        1 ataaatgcta attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa
       61 atttatgaaa aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtcaccatt
      121 aatttctcag gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta
      181 tgagactcga ctcaaagtca gtgagggatg gcaaaatgga tggtggggaa gcatggaaag
      241 taatacctgg aataccattc atgaaaataa aaaggaaaat gccgcactca atgatgttca
      301 ggttgaagtt aattacgcga ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt
      361 aacgcatttt agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc
      421 gacaaaagat cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca
      481 agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta
      541 ccatattaat agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaacga
      601 ttatcgctat gcaaaccata agaaatgggc gacggaaaat gcatttgttc tacaatacca
      661 tatgacgccc gatattacgc catacataga atatgactac ctggaccgtc agggtgttta
      721 caacggcaga gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta
      781 gtagacagga gacagtcaca atgaataaaa caataacggc gcttgctatc atgatggctt
      841 catttgccac aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg
      901 gagcaattga taacgacact gtctacattg gtttaggtag cgcaggtacg gcatggtaca
      961 agctggatac acaggccaaa gataaaaaat ggacagcgtt agctgcattc cctggtggac
     1021 caagagatca agcaacctcg gcatttattg atggcaatct gtatgtgttt ggcggcattg
     1081 gcaaaaacag cgagggcttg actcaggtat ttaatgatgt acacaaatac aaccccaaaa
     1141 ccaatagctg ggttaaattg atgtcgcacg cgccgatggg catggcgggc catgtgactt
     1201 ttgtacacaa cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct
     1261 attttgaaga tctcaacgaa gctggaaaag attcaaccgc tgtagataaa atcaatgcct
     1321 actattttga caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc
     1381 cctcaacaca gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg
     1441 cggttgtgaa taaaggtgat aaaacctggc ttattaatgg cgaagccaaa cccggattgc
     1501 gaacggatgc cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg
     1561 ctcccgtctc atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc
     1621 ttatatttgc cggaggggcc ggattcaaag gttcacgaga aaattaccag aacggtaaga
     1681 actatgcgca tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg
     1741 ggaaatggga taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct
     1801 ggaataatag tctattgatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt
     1861 cagttttgat ctctgtgaag gataataaag ttacagtaca aaactaacgc ttcagggccc
     1921 cagtaaaggg ccctgctatc aatttgtcat ttcaattacg cgaaatttat atgaacgcaa
     1981 taatatcgcc tgattattac tatgttctta ccgttgctgg tcagtctaat gccatggcgt
     2041 atggcgaagg actgccatta ccggacaggg aagatgcgcc tcatcccaga attaaacaat
     2101 tagcgagatt tgcgcatacg catcccggag gcccgtcatg tcactttaac gacattattc
     2161 cactgactca ctgcccacac gatgttcagg atatgcaggg ttatcaccat cctctggcaa
     2221 cgaatcatca aacacagtac ggcaccgttg gccaggcact gcatattgca cggaaattac
     2281 tgcactttat tcctgataac gcaggggttc tcatcgttcc atgttgccgt ggcggatcgg
     2341 cttttaccgc gggcagcgag gggacatatt cagaacggca cggagccagc catgatgctt
     2401 gtcgttgggg aacggatact ccgctgtacc aggatttagt cagcagaacg cgagccgcac
     2461 tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg gatgcaaggc gaatttgact
     2521 taatgaccag tgactactcg tcacaccctc aacactttaa tcatatgatt gaagcctttc
     2581 gtagggatct aatacaatac cattcgcagc ttaataaaat tactgacgcg ccgtggtttt
     2641 gcggcgatac cacctggtac tggaaagaaa atttccctca tgcgtatgaa gctatttatg
     2701 gcaattatca aaataatgtt ttagccaata ttattttcgt cgacttccag caacaaggtg
     2761 aaagaggact gacgaacgcg cctgatgaag atccggacga tttaagcacg ggatattacg
     2821 gttcagcgta ccggtcaccg gagaactgga cgacggcact gcgaagcagt catttcagct
     2881 cagcagcccg tcgggggatt atttctgaca ggtttgtaga agcaattttg cagttttggc
     2941 gcgaaaagtg agcgttcatt aatttgattt attcgcatcc atcaacgctt ttaactcctc
     3001 cggcgtgcgg cactataacg acttccacaa caaggagcca ttttcttata ccactttcag
     3061 tgaccatccg cttatgccac caccctctac gcaactgttt gcagagcacc ttcccaccga
     3121 gtggatacag cacttcctga cgctttctgc tcatgcgacc gttcgtcgtc ttaacctgag
     3181 cgtggatggc gaagcgggga tgaacctgct ggcgcccgca gcgctgtcac ccaggcgcgt
     3241 cagcgcgtgg gggccgcccc ggtggaatgg ctcttccgcc agaccacaca gacatggggc
     3301 gcggaacgtt acctgaagga tgactggcac ggcctgcaac tttttgccat tgatggcgca
     3361 cagttcagga cacctgatga acccgagctg cgtgaatatt atggctctgc caacacatcc
     3421 actgagcggc agagcgccta ccccgtcatg cgtctggtgg cgttaatgaa tctgggaatc
     3481 acattctact gaatgccatg accgcgccta ccggcggagc gatacggcgt gaggcaacaa
     3541 aagcggcgtc agagatcagc tttaagttcg cgttccagtt tatcgtgaca gaaatgatag
     3601 tgctgggaaa cacggcgtca ccggggacta tcccgaaacg actggagcat ctgcgggggc
     3661 tctggaagtg gtgttcataa caaaacgccc ccgaccatca agggcgaggg cggttaagat
     3721 atcaaaaacc cgttatcctg tgaaacacag tgccgccccg cttaagtgaa cggcattgtg
     3781 tttctgaggg accttaattt tccctgattc tctgacgttc cttaccattg atatttcctt
     3841 cattcctcac cagagaaata aaaaatcact atac
//
LOCUS       DAZUCE010000331         3874 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZUCE010000331 DAZUCE010000000
VERSION     DAZUCE010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.333"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(1..672)
                     /locus_tag="ACHY03_004870"
                     /pseudo
     CDS             complement(1..672)
                     /locus_tag="ACHY03_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
     gene            complement(1346..1870)
                     /locus_tag="ACHY03_004871"
     CDS             complement(1346..1870)
                     /locus_tag="ACHY03_004871"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFS3907988.1"
                     /translation="MAQLSRTPSLLNHASEWITLSGQQITRLTELPPAYNLQRSAQLL
                     QQLRGLFPDNPRVQEMVDNWQKSVRSRALPEEAMTGWNEGMTRLQQLAERLNRLDEQR
                     GKYMTVSELKTEVFGIMQAFNRHIPAEEQLRRYDEARNQNGSEQQQKQAEMALNQLIN
                     RYQMEHTGNQERQP"
     gene            complement(1922..2071)
                     /locus_tag="ACHY03_004872"
                     /pseudo
     CDS             complement(1922..2071)
                     /locus_tag="ACHY03_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2396..2710)
                     /locus_tag="ACHY03_004873"
                     /pseudo
     CDS             complement(2396..2710)
                     /locus_tag="ACHY03_004873"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            complement(2765..3070)
                     /locus_tag="ACHY03_004874"
     CDS             complement(2765..3070)
                     /locus_tag="ACHY03_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907989.1"
                     /translation="MNPLFNDIQMRLFYLNHAPYSWHWNVRFRPQEAVYIGNDACHIT
                     ITCNQSGFHLTRDGQRLFTERYIRTLSELLAVLKRRWDVTPAIIRAVEYLSRVPVSH"
     gene            complement(3123..3572)
                     /gene="tssE"
                     /locus_tag="ACHY03_004875"
     CDS             complement(3123..3572)
                     /gene="tssE"
                     /locus_tag="ACHY03_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFS3907990.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTSILQGMPGTAHQLMRVLSDVLLKYEPRIKRVGVTMQEQSQPG
                     ELHYVIDAELKDAGLVRYGTTFMPEGRVLLRYLKQQQFVDNVMTFRG"
     gene            complement(3575..>3874)
                     /gene="tssJ"
                     /locus_tag="ACHY03_004876"
     CDS             complement(3575..>3874)
                     /gene="tssJ"
                     /locus_tag="ACHY03_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFS3907991.1"
                     /translation="NRNFDSADYQALFTGDSEVLAGDIIAQKDVWLQPGGSVAVDMPL
                     DDAAKFTGVAAMFLEPDRKKNTWRVVLSLDELEPDTPRLIEVSGNTLTLLPVKDK"
BASE COUNT          956 a         1027 c          856 g         1035 t
ORIGIN      
        1 tgccactgtt ttattctgaa ttaatatatt ggcaacaata ttgagaatag tctgtgtttt
       61 acctgtccca ggtggtcctt caataactga aatctgtgag gaaaatgccc gttcaaccgc
      121 cattaactga ctctcattca ggccaaacgg aaaaatcaac ccccatggcg aatcacgttt
      181 tttactctgt cctgaacagt atgcatgcaa ggcggtatcc ggatgaggaa ctattttctt
      241 tatctgtctg aggatattct catcgatatt tttattatta cctcgagcat aaaaaacacg
      301 ttcgtcagcc accctgcaca agtaattaaa cacggcatgc tctgtaaatg ctgaacactg
      361 ttaaaagtca agtccggtcg ttttcatgat gtagggtcgt tcgctctcac ggtaataaat
      421 agcagcatat ttatcaccat agataataac ccgttcagcc cgggatgtta cagcgttccc
      481 tttcctttcg aggaaaacat ttcgcaaaat tacagttggc gttatctgac atacagaaag
      541 cggacgagta tattttttcc cgctgcgaaa atgacaggtc agcataacct ctccgtcatc
      601 atttccagcc cacagagtcc agtcggtaat tttatgagtc ttattttcac cgtcaacaca
      661 aatcgtgacc atattctcac tcagtaatta tcatttaact tacggaaaat tgttatgaaa
      721 ctcccgatat ttacccggta aaatacttgt cattgtttga acccctccct aaaaaatgac
      781 caggcatttg ataacccggt gtatttctcc cgatatgagc tccggaggat tttccacaac
      841 tccgggtgtg ttaacatatt cccgccggcc atatatacat tgagttcagt cataacagtc
      901 aataccagga ttacaggaac gcgatgctga tacgctggtt attgaatact ggtagcccct
      961 aactactaat tcatcacgat actctgtact tactgttttt tacaaccatt cccccaggta
     1021 aaagagtcca tttatcatgc ccctctttaa cgcctgccag cataatttaa gaatggatac
     1081 cgtgggtgga attaacaata cctgctcagg ccaatgccat gtaaccgcct cactgaaaaa
     1141 gccagatcat ctaaccaggt acagaccaac atcgcaaaaa ttaccatgta gcacgaactg
     1201 ctgtcatatt tctgttcagg tatgccgcta tttcgcgggt tttcttcacg ccataatgcg
     1261 tttcaaggta gcgaacatct tcgattgtcc atggtgtgcg cgctcttccg ggggtatttt
     1321 ccatttccat gttttatctc agccattatg gctgcctctc ctgattgcct gtgtgttcca
     1381 tctgataacg attaatcagc tgattcagtg ccatttcagc ctgtttttgc tgctgttcac
     1441 tgccattctg attacgggct tcatcatagc gacgtaactg ctcttctgcc gggatatgcc
     1501 ggttaaaagc ctgcatgatg ccaaacacct ccgttttcag ttcactgacc gtcatgtatt
     1561 ttccccgctg ttcatccaga cggttcaggc gctcagccag ctgctgtaag cgggtcatgc
     1621 cttcgttcca gcccgtcatc gcctcttccg gaagcgctcg gctccttacg ctcttctgcc
     1681 agttatccac catttcctga acacggggat tgtcggggaa aagtcccctc agttgctgca
     1741 gcagctgcgc actgcgttgc aggttgtatg ccggcggtag ttctgtcagt cgcgttatct
     1801 gctgaccgga aagggttatc cactctgacg catgatttaa cagtgagggt gttctgctga
     1861 gttgcgccat ctggtctgct gaaagcacat ccggtagtgg tgccagcagc gccctgagtt
     1921 gctgctccgc cggtgatggc tgtgtcagaa actgccatcc cggaatgacc acgctgacag
     1981 tcagcaatgc cgcacagaca cccgccacaa acggtttcag aaacgtgggc ggggatttct
     2041 gaagggaggg tgtgggttct gcttttgttt cagaaacaac atacaccaga cgggagtacg
     2101 gcaggggctg atgttcctgt acatccggag catcagaccg gagtgcctga gccggaaggg
     2161 ttaccggcga cgatacttca ccctgcacag gctgattctc cagacgggtg atatatccct
     2221 tcaccatggc ttcaggtaaa gccactttac tgctctgttt aagttcatgc cgggtcagta
     2281 tgtcactcag cgttttcagg catgtttctg tctgatacag caggggcagg ttgtcacgct
     2341 catccggtgg catggcccgg agcgtttttt gcagacgact gaacagaccg gtaataattt
     2401 ccagccgggc gtgggtattc agcggccaca tcactggcca gtgatgccgg gtcagcgcca
     2461 caatcagagc cagcccttca ttcaggccac ttaaccctgt tgtatgcatc cgggcgatgg
     2521 tgtaccagga tgccgtctgt aattccacgc cattgtgttc atacagacgc aggcagagcg
     2581 tctccacata acgccagtcc acatccggac gtgccgggtg agtcagcttg ctgatttcat
     2641 cgcgcagggc agcataatcc ggtaatgacc gggggtcatt acctgttttc agttttactt
     2701 cagaagtcat cggcagattc agtgttgctg tatcaaatca taattaatat tcccggaaag
     2761 agcgtcagtg tgataccggt acccgggaaa ggtattccac cgcacggatg attgccggtg
     2821 tcacatccca ccgccgcttc agaaccgcca ataactcact gagtgttctg atataacgtt
     2881 cagtgaacag tcgctgcccg tccctggtta aatgaaaacc ggactgatta caggtaatgg
     2941 taatatggca ggcatcatta ccaatatata cggcttcctg tggcctgaat ctgacattcc
     3001 agtgccagga atacggtgca tgattcagat aaaaaaggcg catctggatg tcattaaata
     3061 acgggttcat cattcagccc tcgtttatgt ctgttaagca cacggataag gggaaccata
     3121 aatcaccccc tgaaagtcat cacattatcc acaaactgct gttgcttcag atatcgcagt
     3181 aacaccctgc cctccggcat aaaagttgtt ccgtaacgaa ccagtcctgc atctttaagt
     3241 tctgcatcaa tgacataatg caactcacca ggctgactct gttcctgcat cgtcaccccg
     3301 acacgcttaa tacgtggctc atacttcagc agaacatctg aaagcacacg catcagttga
     3361 tgcgccgttc ccggcattcc ctgcagaatg cttgtcatat ccggcagacc ataatccgga
     3421 agatgtttaa ggcttccggc ccgggtattc agaatacgct gcatgttatc gagcaccgaa
     3481 agaataacct gctcttccgc actgacctgg tttaactcca gcccgccagc gaaattgcca
     3541 tagagaattt cataaaggga aggacgcggc atcactattt atccttcacc ggtaacagcg
     3601 tcagggtgtt accggatact tcaataagac ggggcgtgtc cggctcaagc tcatccaggc
     3661 tcagcaccac tcgccaggta tttttcttcc ggtcaggctc cagaaacatt gctgccacac
     3721 cggtaaattt cgccgcatca tccagaggca tatccacggc cactgaacca cctggctgca
     3781 gccatacatc tttctgtgca atgatatcac ctgccagaac ttcactgtcc cccgtaaaaa
     3841 gcgcctgata atctgcactg tcaaaattcc ggtt
//
LOCUS       DAZUCE010000332         3872 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZUCE010000332 DAZUCE010000000
VERSION     DAZUCE010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.334"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(67..411)
                     /gene="yoaB"
                     /locus_tag="ACHY03_004877"
     CDS             complement(67..411)
                     /gene="yoaB"
                     /locus_tag="ACHY03_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFS3907992.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            543..2453
                     /gene="yoaA"
                     /locus_tag="ACHY03_004878"
     CDS             543..2453
                     /gene="yoaA"
                     /locus_tag="ACHY03_004878"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFS3907993.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2511..3206
                     /gene="tsaB"
                     /locus_tag="ACHY03_004879"
     CDS             2511..3206
                     /gene="tsaB"
                     /locus_tag="ACHY03_004879"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFS3907994.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAALPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGVWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3246..3827
                     /gene="yeaY"
                     /locus_tag="ACHY03_004880"
     CDS             3246..3827
                     /gene="yeaY"
                     /locus_tag="ACHY03_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFS3907995.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLAQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
BASE COUNT          888 a         1001 c         1092 g          891 t
ORIGIN      
        1 gatttatgta aaaccgacca gtattatcga taaattatcg tctgacttat tttgcttcga
       61 taaagcttac accgcagcca caatcttaat ttcaacttta tacttcgggt tcatcaaacc
      121 cgcttgtacc gtgcagcgca ccggcgcatg acccgcgaca acccaggcat cccacgcttt
      181 attcatcgcc gcgaagtcgt ttttatcggc caggaaaatg gtggcatcca gaatactcga
      241 tttattgctg ccctgttttt ccagcacggc gtcaatctgc gccagcgtgt tggcggtttg
      301 ctcaaaggca tcggcgtcga ggttttccgg tacaccagtg tagtagagcg tgttgttgtg
      361 gattactaca tcagaccagc gggcttcagc atcgatacga acgatagtca tcattttgtc
      421 ctcattatcc ttccattttc gtgctgcaaa gactgccaca atgtgaggtc gccgtcacca
      481 cttcaactgg cgaaagcgcc ctcatcctga cataatccct gttcaaatca acagggggta
      541 gtgtgacgga cgattttgca ccagacggtc agctggcgaa agcgatacca ggctttaagc
      601 cgcgagaacc acagcgacag atggcggtag ccgtcaccca ggcgatagaa aaaggccagc
      661 cgctggtggt ggaagcagga accggtacgg gcaaaaccta cgcttacctg gctcctgcgc
      721 tgcgggcgaa aaagaaagtc attatctcga ccggctcaaa agcgttgcag gatcagctct
      781 acagccgcga tttgccaaca gtctcaaagg cattgaaata tacgggcaat gtggcgctgc
      841 tgaaagggcg ctcaaactac ctctgcctcg agcgtctcga acagcaggcg ctggcggggg
      901 gcgatctgcc ggtacaaatc ttaagcgatg tgatcctgct gcgctcctgg tctaatcaaa
      961 cagtcgatgg tgatatcagc acctgcgtca gcgtggcgga agattcacag gcgtggccgc
     1021 tggtcaccag caccaacgac aactgtcttg gcagcgactg cccgatgtat aaagattgct
     1081 ttgtggtcaa agcacgtaaa aaagcgatgg acgccgatgt ggtggtggta aaccatcatc
     1141 tctttctggc ggatatggtg gttaaagaga gtggatttgg cgagttgatc ccggaagcgg
     1201 acgtcatgat cttcgacgaa gcccaccagc taccggacat tgccagccag tattttggtc
     1261 agtcactctc cagtcgacaa ctgctcgacc tggcaaaaga catcaccatc gcctaccgca
     1321 ccgaattaaa agacacccag cagttacaaa agtgcgctga tcgtcttgcc cagagtgcgc
     1381 aggattttcg tctgcaactc ggtgagccag gttatcgcgg taacctgcgt gagctgttag
     1441 ctaatccgca aattcagcgg gcatttttac tgctcgatga caccctggaa ctttgttatg
     1501 acgtggcgaa actgtcactg gggcgttccg ccttgctgga tgcggcattt gagcgcgcca
     1561 cgttgtatcg cacacggctg aagcggctaa aagagatcaa tcagccgggc tacagctact
     1621 ggtacgaatg cacttcgcgc cattttactc tggctctcac gccgctcagc gtggcggata
     1681 aattcaaaga gttaatggcg caaaagcccg gtagctggat cttcacctca gcaacgctgt
     1741 cggtgaacga cgatctgcat catttcacct cgcggcttgg gatagaacag gcggagtcgt
     1801 tgctattgcc aagcccgttt gattacagcc gccaggcgtt actctgtgtg ccgcgcaacc
     1861 tgccgcaaac caatcaaccg ggttccgcgc ggcaactggc ggctatgctg cgcccgatca
     1921 tcgaagctaa caacggtcgc tgttttatgc tttgtacctc gcacgccatg atgcgcgatc
     1981 tggccgagca gttccgcgct accatgacgc ttcccgtatt attgcagggg gaaaccagca
     2041 aagggcaact gttgcagcaa tttgtcagtg ccggtaacgc gcttcttgtg gcaaccagta
     2101 gtttctggga gggggtggac gtgcgtggcg atacattgtc attggtgatt atcgacaaat
     2161 tgccgtttac ctcgcctgat gatccattgt taaaagcgcg catggaagat tgccgtttgc
     2221 gcggtggcga cccgttcgat gaagtacaac tgccggatgc ggtgattact ctcaagcagg
     2281 gagtcgggcg actgattcgc gacgccgacg atcgcggggt tttggtaatt tgtgacaatc
     2341 ggctggtgat gcgtccttac ggcgcgacgt ttctcgccag tctgccgccc gcgccacgca
     2401 cccgtgacat tgcccgtgcg gttcgtttcc ttgcgatacc atcctccagg taatttgtta
     2461 cggagtatgg tatgatgcgc gcctttttat tgatctaagc acgaagaccc atgcgaattc
     2521 tggctatcga taccgcgaca gaggcctgct ctgtcgccct gtggaacgac ggtactgtca
     2581 acgctcattt tgagctttgc cctcgtgaac atactcaacg aatcttaccg atggtgcagg
     2641 atatcctgac caccagcgga acttccctga ctgatattaa cgctctggct tacgggcgcg
     2701 gccccggtag ctttaccggc gtgcgcattg gtataggcat tgcgcagggg ctggcacttg
     2761 gcgcggcgct gccgatgatt ggcgtctcca cgctcatgac gatggcgcag ggggcgtggc
     2821 gcaaaaacgg cgcaacgcgc gtgctggctg ccattgatgc gcggatgggc gaagtttact
     2881 gggccgaata tcagcgtgat gaaaacggcg tctggcacgg cgaagaaacc gaagccgtac
     2941 tcaaacccga actcgttcat gaacggatgc aacagctttc cggggaatgg gtgacggtag
     3001 gtacaggctg gcaagcctgg ccggatctcg gtaaagagag cggactggtt ttgcgcgacg
     3061 gagaagtttt actgcccgct gcagaagata tgctgccgat tgcttgtcag atgtttgccg
     3121 agggtaaaac ggtggcggtg gaacatgccg aaccggttta tttacgtaac aacgtcgcat
     3181 ggaagaaact tccgggcaaa gaatgaatct taagagtatg ccctgagaaa aaaaggagtc
     3241 gcatcatggc ggttcaaaag aatgttatca aaggcatact ggcaggtacg tttgcgctaa
     3301 tgctgagcgg ttgtgtcact gtgccggacg ccattaaagg cagcagtccc acgccgcaac
     3361 aagatttagt tcgggtgatg agtgcgccgc agctgtacgt tggtcaggag gcacgctttg
     3421 gtggcaaagt ggttgcggta caaaaccagc aagggaaaac ccgcctggaa attgctaccg
     3481 taccgctgga cagcggagcc agaccgacgc tgggagaacc ttctcgcggt cgcatttatg
     3541 ccgatgtgaa cggttttctg gacccggtgg atttccgtgg acaactggtt acggtagtcg
     3601 ggccaatcac cggtgcggtt gacggcaaaa tcggcaatac gccctataaa tttatggtga
     3661 tgcaagtaac gggttacaaa cgttggcatt tagcccagca ggtgattatg ccgcctcagc
     3721 cgattgatcc atggttttat ggcggtcgtg gctggcccta tggctacggc ggatggggct
     3781 ggtataatcc cggccccgcg agagtacaaa cagttgtaac tgaataattg cttgttttta
     3841 aagaaaaaga aacagcggct ggtccgctgt tt
//
LOCUS       DAZUCE010000333         3869 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZUCE010000333 DAZUCE010000000
VERSION     DAZUCE010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.335"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(111..238)
                     /locus_tag="ACHY03_004881"
                     /pseudo
     CDS             complement(111..238)
                     /locus_tag="ACHY03_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(293..421)
                     /locus_tag="ACHY03_004882"
     CDS             complement(293..421)
                     /locus_tag="ACHY03_004882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907996.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(782..975)
                     /locus_tag="ACHY03_004883"
                     /pseudo
     CDS             complement(782..975)
                     /locus_tag="ACHY03_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1295..1600
                     /locus_tag="ACHY03_004884"
     CDS             1295..1600
                     /locus_tag="ACHY03_004884"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3907997.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVFIVATCVIIQVSLERTV"
     gene            complement(1789..2027)
                     /locus_tag="ACHY03_004885"
                     /pseudo
     CDS             complement(1789..2027)
                     /locus_tag="ACHY03_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            2298..2951
                     /locus_tag="ACHY03_004886"
                     /pseudo
     CDS             2298..2951
                     /locus_tag="ACHY03_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063945310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(3361..>3869)
                     /gene="atoB"
                     /locus_tag="ACHY03_004887"
     CDS             complement(3361..>3869)
                     /gene="atoB"
                     /locus_tag="ACHY03_004887"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFS3907998.1"
                     /translation="EALGALRPAFDKAGTVTAGNASGINDGAAALVIMDESAALAAGL
                     TPLARIKSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVG
                     KTLGFDPEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGI
                     AMVIERLN"
BASE COUNT          955 a          876 c          939 g         1099 t
ORIGIN      
        1 gtatttaaac catcttttca tatttattta acataatgca gattctgatg tattagttca
       61 gttgttcgcc agaatcagcg ctcgatcact atgcgtttta gcagtgtgct gaagaagttc
      121 tccatcactg cgttattcag accgtttcct ttgtgcggca tattttgtgc tactccatgg
      181 ggctttaact ttgcctatag cctgcgattc gatattgcca accctgatcc gtatgcagcg
      241 acgacggggc atcctctggc cgggcactgt gaatgcatcg tatagcatcg tattaacgat
      301 gctccatcac cgacctttct gacaagctgt aggagataat ttcccggtta aacagatcgg
      361 agaactggcg acaggtacca cttttcccct gtaccgagaa ctctgtaaca tccgccccca
      421 tttttcgttg gccttcgtca aagttccggc tcaggatatt gggcgcagat cacttatttg
      481 gtggacacca ctttgtcgga tgcttcagat tatgaccaga tggatttcgc tggacgctta
      541 cgattacatg caccatattg tcaacgatta cgccactgag tgtatgacac acattaaagc
      601 gttaggctca ggccttatta caatgtcatg ctagtgggct tgtacgccgt aaatatctgc
      661 atgataccag aatgaccggc aaacaaaggt gccccggtaa gctcgtcagt ggatataaag
      721 aaaatcaagc gcgctgataa cgatgccaca tctgtggttg gtggcaatat gcagattgtt
      781 gttatttgtc ttcattgcat tctaagaagt gtttcttatc aggcctgatt ttttgccatt
      841 atttttaatg aggtcttcaa gtggaaaata gattaggcgt tcatgtggca gcgcggcacg
      901 tccccattta gtggcaaact cccgttggcg tgcctgtaac agttgctcca gcctttttat
      961 tctgttctca ttcatccggt tttcctcgta tgctgcatca caccactgtg ctaatgagaa
     1021 atgtaatcag tgtggtggac gtattttatc cgcattggaa ttgtcgtcaa ccaaaggaga
     1081 tgagacccgt ttgtatcgca atgggtctat ggcgctcatg cccgtttgct gttcgataat
     1141 ggcttctctg agcatacggt gttgcagttc tcgtaaaaac tttgcgtctt ctggtgataa
     1201 atccattgtt attactcgta aagcaatgcg ctgtagtgcc ttacgctacc gcgctgatgg
     1261 ttcttatccg ttttattgaa taggagaaca aactgtgagc tatcagcctg gtggtctggt
     1321 tttgcctgat ttaagccgcc gtgggtattt gtccaagaat gcttcaagct ccaatcgttt
     1381 tggcctggtg aatggagatg ggttaagcaa agactctcgt tgctggtgta ctttttcgcg
     1441 ctggcgctca agcatggata atgcgattgc ctgtagttct tcgttagtca ttgtctgcac
     1501 ttggaaagca atttaccgga gtacctcaca ctacggcaaa aatgttttta ttgttgcaac
     1561 ttgcgtaatt attcaggtca gtttggaacg cactgtataa acataaataa cctgatactg
     1621 ttgtgtgttt tcaaagaaaa cacaggtatt tttcacataa actttcccct tttcatatcc
     1681 gaccacttaa tcaaaaaacg gttttatact tgttgccccc actggggtta aatgtgagac
     1741 atgtcgcact ttgtgccatg tgtcctcgct gattttaggc gcggttcatt cattctttct
     1801 attggcataa agtatggtat cggttgggcg aatgaaaggg actcgggtca tggtcagaca
     1861 atacctgagg cgttaattcg ttgaaagaga ttgtgaatgg gggaataact gagtcttaac
     1921 tgctgtgcga tagcccggac agataattgc tcgtcgaatc tgagacgtaa tgagtctggc
     1981 aaataagtgc gaaagccgat gatatctagt gttgtttgag agttcatcgt tcgagtccat
     2041 taatacagat ggacgcgatt gttgccagac aggataattt tcacaagaca tcgcagatgg
     2101 agtacttacg ataatttttt attttataca acagatgccc acgaaaagaa ttattttcaa
     2161 tagacacctg cattacacaa agtagataca tacgcagtca gagtacactt actttttatt
     2221 atttcctgca atggcgatta cggctattga cagtaatgaa attattagtg aaccaagacc
     2281 taaagtagtc ccgggaccaa caccttcgtc ccttccgggg tatcctgcag gcggatgcgt
     2341 tcagcggtta cgatcggctg ttcagtgccg aacgtgaagg tggtgcactg acagaagttg
     2401 cgtgctgggc ccatgcccgg cgaaagatcc acgatgtata catcagcagc aaaagtacga
     2461 cggcagaaga agctctgaag cgcatcagtg aactgtacgc catcgaggat gaaatacggg
     2521 gcttaccgga gtctgaacgt cttgcagcca ggcagcagcg aagcaaagcg ttactgacgt
     2581 cgctgcatga atggatggtg gagaagaatg gcacgttgtc gaaaaaatcc agactgggcg
     2641 aagcgttcag ctatgtcctg aatcagtggg acgccctctg ttattacagt gatgacggtc
     2701 tggcggaggc ggacaataac acagcagaaa gagcgcttcg tgccgtctgt ctcgggaaaa
     2761 agaattacat gttcttcggc aacgatcatg gaggcgagcg tggagctctg ctgtacggac
     2821 taatcgggag ctgtcggttg aacggtatcg atccagaagc gtatctgcgc catatcctga
     2881 gcgtactgcc ggaatggccc tccaaccgtg ttgacgaact gctgccatgg aacgtagttt
     2941 atcaccgata aataagtgtc aatacggcgc tctgttgacg cttacgattt tccttcaacc
     3001 agcgcaacgt ttcgtggcct tttgtggatg atataagtta ttgacaatga atgtaatcgt
     3061 tttgcccatc ggtacgttgc tttctgatgt tttaacagag acggataaat aagcctgaac
     3121 ctttgctgtt tcctgcaacg taatggattt tgatgtgagg aatttgagat ggtgtttatg
     3181 aattatacaa ataactggga ggaggcgact ctctctaatt gctgttcgtc tctccaatta
     3241 tatttacaaa tgaacatctt ctgctctaat cctgtttcag taaggactca agttacactc
     3301 tgacgagttt ttatgaggcc tgtgaaacca cctcctatgg actggaggtg gcttctgctg
     3361 ttaattcaat cgttcgatca ccatcgcaat cccctgaccg ccaccaatac acagtgttgc
     3421 gaggcccagg gttttatcgc gtgcctgcat tgcatgtaac agagtgacca gaatacgtgc
     3481 accactggca ccgataggat gcccgagcgc gatggccccg ccgttgacat tcactttctc
     3541 aggatcaaag cccagggttt tccctacagc aaggaactgt gcagcaaatg cttcattagc
     3601 ttcgatgaga tcaatgtctg ccagttgcag ccccgccagt tgtaacgctt tttgcgtggc
     3661 aggtactggc cccataccca tcaatgcggg gggcacgcca ccgctggcat aacttttaat
     3721 gcgagccagg ggggtaaggc ctgctgccag cgccgcagat tcatccataa tcaccagagc
     3781 ggcagcaccg tcgttaatgc cagacgcgtt cccggcggtg actgttcctg ctttatcgaa
     3841 ggccgggcgc aatgcgccta acgcttcag
//
LOCUS       DAZUCE010000334         3827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZUCE010000334 DAZUCE010000000
VERSION     DAZUCE010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.336"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1085)
                     /gene="tssA"
                     /locus_tag="ACHY03_004888"
     CDS             complement(<1..1085)
                     /gene="tssA"
                     /locus_tag="ACHY03_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFS3907999.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKDIRVATWYIWARLRQDGEK
                     GLADGLELLTGLLQRFGEHLHPQRSRARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGALWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMP"
     gene            complement(1085..>3827)
                     /locus_tag="ACHY03_004889"
     CDS             complement(1085..>3827)
                     /locus_tag="ACHY03_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFS3908000.1"
                     /translation="LPLYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLP
                     GLVAQGIQQICCAPRYYFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPAT
                     VGGERSVRHRWRMDNRWEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTG
                     MVVSFLANRSLVAEVQEQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYL
                     RAGMNQNADLLAVVMPLYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAA
                     YDQLRLYLMLTQPQHMEPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITH
                     PQWKLTADEELVSQSRTLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDV
                     SRLFFTDEVVPGMFTRQAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEAL
                     RQRLTTRYFADFGNAWLNFLNSLHLRKAQTLSDVTEQLTLMADVRQSPLVALMNTLAV
                     QGRTGQPREAVTDSLVKSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNS
                     ADMLNLQTYLTRVTQVRLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLT
                     AAGLGQEWYGFGQTVFVRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFK
                     NVSSDASLPLLAKYLNTDTGRIARFLQNNLSGVLHSEGSRWVPDTINTRGLTFNPAFL
                     KAINTLSEIADVAFTTGNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFT
                     WPADTEAPGASLSWVSTQAGTRQYADLPGSWGLIRLLEMARRKAASGVASGWSLSWQA
                     QDGRMLNYTLRTEAGEGPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDSVEETD"
BASE COUNT          865 a         1162 c          975 g          825 t
ORIGIN      
        1 ggcatgccgt cgttaaaagc cagtgtctca agccccggca ggcgcagaag caatcctttc
       61 aggtcatact gaataatggc agcataattt tcttttccgg tctgcaacag cgcctgatga
      121 atataccact gcaggtccag ccacagatga ctggctcccc gggcaaacat atcgtcacac
      181 tgttcaagca gtgacaacca gttctgctgc agatacaacc gtttcagtga cgcacgccgg
      241 tccggccccg gtggggcaat acgggtccgt ccatcagcag ataacggcgg taactgatgc
      301 agcgtgtcat gccggacgct tttcatcagt cgatgagccg ccagccagcc ctcaggctgg
      361 tcacgcagat attttgccag aacacgggcc tgtgacagca gttcctgtcc ggaggtgatg
      421 gcagataatg ccatgacgct gccggatgtt accgtcggtg ccggcgcggc agcctcctga
      481 ggcaccactg catcaacacc accggccttc atcaggcgat tttcaagtgc ctgatataac
      541 ccgttcagca ccggggcgga agcctcatcc gtaaaggtct gttctgccag ccacagcgcc
      601 ccggcaatgc gcagggtatc cgccttcacc acctccggat acagagacag actgtccagt
      661 atgcgggcgc tgcataacca ctccagcgcc gccttcctgg cccgtgaccg ttgtggatgc
      721 aggtgctctc caaaacgctg caacaaaccg gtcagcagct ccagaccgtc ggccagccct
      781 ttttcaccat cctgccgcag acgggcccag atataccagg tcgccacccg gatatctttg
      841 gttctggtgg tcagtaattt ttctgcaagc tggctgacaa ttccggcatc ggctccggag
      901 agtttattga cctcttcacg catctgctga aaatcatcat cgtaccccgc atcatcacct
      961 gcgggattat ctggcgtcac gggagcaagc catttatccc acagcgcaat acgcacctga
     1021 gccggttcag ccacatcccg gggtgtcgtc tggcatgcac tgaggagcga atgaatggaa
     1081 gccatcagtc agtctcctcc acggaatccc cggcatcttc gtcattgcct gtaaacgctg
     1141 acgtaccgga gagttcgaaa accgtttccg gcagaacaaa attgcggagt ttcagcaata
     1201 caagcggccc ttcccccgct tcagttcgca gtgtgtaatt cagcatacga ccgtcctgcg
     1261 cctgccagct caggctccag ccactggcaa cgcccgatgc ggctttccgt cgggccatct
     1321 caagcagacg tatcagcccc cagcttcccg gcaggtctgc atactgacgg gtaccggcct
     1381 gagtgcttac ccagcttaaa ctggcgccag gtgcttccgt atcagccggc caggtaaatc
     1441 gcttccatac cggcatctga ttaacataaa tcagtttctg attatcggtt atcagcgtcg
     1501 tctgcatcac accggcagca gttcccgggc gcagctcaaa atgcagcccc gcgttacctg
     1561 tggtgaacgc cacatccgct atttcactca gggtattgat ggctttcaga aacgcagggt
     1621 tgaatgtcag tccccgggta ttaatggtat caggtaccca gcgactgcct tcactgtgca
     1681 acacaccact caggttattc tgcaggaaac gggcaatacg tcccgtgtcc gtattcaggt
     1741 attttgccag caggggaagc gaagcatcgc tgctgacatt tttaaacgga taacgcccgg
     1801 aaaatgcatt attccagcca tccaccaccg cagtacgcca ccgggcattc agactttctg
     1861 ccgcgggcgt aagaacctgc tgccacgcct gctccatcgg gcgaacaaag accgtctgtc
     1921 cgaagccata ccactcctgt ccgagtccgg cagcggtcag gctaccgtaa tcccgggtat
     1981 cggttaaatc aaccgatttc ccctgcaata ctgtctgtgc cagcatctgc atcatggcct
     2041 gcgggtcaga agagccggca atctgttgca gacgcagacg aacctgtgtc acccgggtca
     2101 gatatgtctg cagattcagc atatccgcac tgttgttctg attatccatc agtgcaagca
     2161 ccggcccgaa agtcgtcgcc agaggaccgt gaagccggct ttccggtact gccactggct
     2221 gtttttcctg tgaaagcagg tttctggcag atttaaccag tgaatcagta accgcttccc
     2281 ggggctgacc agtacgcccc tgaaccgcca gcgtattcat cagtgcaacc aggggggact
     2341 ggcgcacatc agccatcagc gtcagttgct ctgtcacatc agacagcgtc tgagctttac
     2401 gcaagtgcag actgttcaga aaattcagcc aggcattacc aaaatcagca aaatagcgcg
     2461 ttgtcaggcg ctgtctgagt gcttccggcg ataccggtga cggtgccttc tgcctgccat
     2521 ccgtcagcac ccagtccatt tcttcacgac gctcattaat cacggtgtct atcgacggca
     2581 atacggcctc ttcccaggcc tggcgggtaa acataccggg aaccacttca tccgtaaaaa
     2641 agaggcggga gacatcagta tcaccggtca tatccgtcag acgcatatcc gcaaactgat
     2701 gtgccacccg tgccagcatt ttctgataca gcatggcatc gctgttctgt gttcccagat
     2761 ggcgaagcag cagagttctg ctctggctga ccagctcttc atcagccgtc agcttccact
     2821 gaggatgagt aataatcccg gaggcataat acgccagcaa cgtcgggcca ttagcctgcc
     2881 agaaaacggc actcacaccg tcacgctgtg gccattcccg catgagcgta cgactgaacc
     2941 aggcgggctc catatgttgc ggttgcgtca gcatcagata cagtctgagc tggtcatatg
     3001 cggcttttgc cattttcccc cgctgcgggc tgtccggagg aagacgaata aatgtcctga
     3061 gctgctgctc aagatgagca gcagcggcat cacgcagtaa aagatgtgca ttttgtgcat
     3121 aaaggggcat tacgaccgcg agcaggtctg cattctgatt cataccggcc cgcaggtacc
     3181 atggcgcccc gtgctcctcc cggtactgaa ggcgggccag ggatttctgc agattcagca
     3241 acgcctgcag acgttctgca ggagacagtt gctggttctg agccggacga atctgttcct
     3301 gtacttcagc aaccagactc cggtttgcca gaaaggaaac caccattcct gttccctgag
     3361 ccatcatcag tatggccgcc ataacggcca gacttctgcg ccagttatga cctgtgcggg
     3421 aaggctgtag ccggacaggt aattgctgca ctgtctccgg caacgcctcc cagcggttat
     3481 ccattcgcca gcgatgtctg acacttcgtt caccgccgac agttgccgga ctgaaaacaa
     3541 tacccgccag caacaactga cggtatggac ggagtaatac ggataatggc tccaccacag
     3601 catcaatatt tcgtctgaat cgctctgcca gcgacaacaa aaaataatac cgaggggcac
     3661 agcatatctg ctgtatcccc tgagcaacaa gcccggggag catggcctgt agttgtgctt
     3721 tcagtttgtc agaactacat tctgctggca gaaggcatcc cacgggctga gtaatacgcc
     3781 ctcgctcatc cggactctcc tgcagtgacc agacatacag cggcaga
//
LOCUS       DAZUCE010000335         3805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZUCE010000335 DAZUCE010000000
VERSION     DAZUCE010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.337"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1808)
                     /gene="rsxC"
                     /locus_tag="ACHY03_004890"
     CDS             complement(<1..1808)
                     /gene="rsxC"
                     /locus_tag="ACHY03_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFS3908001.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPMAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAADAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVTDPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKL"
     gene            complement(1801..2379)
                     /gene="rsxB"
                     /locus_tag="ACHY03_004891"
     CDS             complement(1801..2379)
                     /gene="rsxB"
                     /locus_tag="ACHY03_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFS3908002.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSELCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2379..2960)
                     /gene="rsxA"
                     /locus_tag="ACHY03_004892"
     CDS             complement(2379..2960)
                     /gene="rsxA"
                     /locus_tag="ACHY03_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFS3908003.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(3037..3477)
                     /locus_tag="ACHY03_004893"
     CDS             complement(3037..3477)
                     /locus_tag="ACHY03_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFS3908004.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(3563..3778)
                     /gene="ydgT"
                     /locus_tag="ACHY03_004894"
     CDS             complement(3563..3778)
                     /gene="ydgT"
                     /locus_tag="ACHY03_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFS3908005.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
BASE COUNT          969 a         1011 c          977 g          848 t
ORIGIN      
        1 agcttgcgcg ctttggcgcg ggcgatagcg gcttcgacgg cggctttgcg cggatcgacc
       61 tgttgttctg gttccgcatt agcttgttgc tgttccagct tgcgtgcttt ggcacgggca
      121 atagcggctt cgacggcagt tttacgtgga tcagtaacgg ttgctgcgtc gttagtttgc
      181 tgcagttctg cctgtttcgc tctggcttgc gctttacggg cttcccgtgc tgcaataatt
      241 gcactgttat ccgggcgttc gcccgcttta atcacaatag gctgtgtagc ttgggcctgt
      301 ttctctttca cccgcgccag agcggcagca atcgcatctt tatctttggc tgcaggttga
      361 acggctgcgc tcttatgtcg ttcaaggcga gccgcttttt cgcgctccag acgagcctgg
      421 cgcgcttcga aacgcgcttt ggcatctgcg gcgcgctttt cttcctgacg aatagccgca
      481 atttcagctt tttcctgacg gaaatattgc accaggggaa tattgctcgg gcaaacccac
      541 gcgcaagccc cacattcaat gcagtcggca atgttatgcg tagtggcttt atcgtgttgc
      601 tgacctttgc tgaaccagta caactgttgc ggcaaaagat ccgcagggca ggcgtcagca
      661 caggcgctac accggatgca gctttgttct tcctgtggtt cgccaagctc attggcagag
      721 ggagccaaca gacagttggt aatctttacg accgggacat ccagccatgg caaggtaaag
      781 cccattagcg ggccgcccat aatcaccatt tgatcggcag aggggcagaa tccggcgtca
      841 ttcaataaat gacgcactgg cgtccccagc cgtgcccaga cgttgcccgg gcgagcgatt
      901 gcttcgccag tcagggttac gacacgctcg gtaatcggct cgccatcaat aacggcacgt
      961 ttcactgcat aagcagtgcc gacgttttgc attaatacgc cgatatcgga tgaacgccca
     1021 ccatgtggaa cctgcttccc ggtcagaatg taggttaatt gtttagcacc gccagaagga
     1081 tatttggttg gaatcacccg cagagaaata tcgtgagagt ccgccagcac cgcgcgcagc
     1141 atggaaatcg cctgcggttt gttatcttca atgccgataa gaatttcgcg tggctgcaga
     1201 atatgcgcaa gaatgcgaat accctctacg acctgagccg cgcaatcctg cattaaacgg
     1261 tcatcagcgg taatgtacgg ctcgcactca gcagcgttga taatcaacgt ctcaatctta
     1321 tctccgccgc cctgcaattt aacgcctgtc gggaatcccg caccgcccag cccggcaaca
     1381 ccaaactgat gaatgcgctc gattaactct tcgcgacggc gagagcgata atcggcccag
     1441 ccgtctcgtg ggatccagca gtcttcacca tcggcatcaa taatcacgct taattcagct
     1501 aaagctgaag gatgagccgt agagtggggc gcaatagccg taacggtacc cgaggtgggc
     1561 gcgtgaacag gcagcatttt gccgcgacca cgggtaagcg gctggccgcg caatacttta
     1621 tcgccgacgc taacgcacaa ctcaccttca gcgccaatat gctgtttcag tggaataaca
     1681 aaacgctgcg ccatgggtac ctggcgcagg ggggtaccgt tggactgggt tttcatctcc
     1741 ggtggatgga tgccgccgtt gaaatcccag attttatttt ttctgaatgc agagaataac
     1801 ttaagcatgg tgttccacgg gaatgatacg cacgggaatg gtgttcagat cccatttcca
     1861 ggagtcaggt gtttctgcga ccggttgcaa cgagatgcag tgcgtcgggc acggatcaac
     1921 acataaattg cagcccgtac agagttcact cattaccgta tgcatggctc gggtagcgcc
     1981 aacgatggcg tctaccggac acgcctgaat acatttagtg cagccaatac agttattttc
     2041 atcaataacc gccaccatcc gcgcaggcgt tagctcttgc gcttcgccat ccagcggctg
     2101 cggttcgaca ttaagcaact cggcaatttt tagcatcaca gcttcgccac caggggcgca
     2161 acggttgatt ttttcaccgt tacagctgat ggcttccgcg taggggcgac agccgggata
     2221 accgcactga ccacactggc tctgcggtaa gatttcgtca attttttcaa cgaccggatc
     2281 gtcttccacc gcaaaacggc gggaggcata acccagaatg gcgccaaacg ccaggcccag
     2341 caggctcacg gcggcaacgg caatccagat agcattcatt acaacttcac caaaccacta
     2401 aagcccataa aggccagaga cataagacct gcggtaatta acgcaatggc attaccgcga
     2461 aaaggagccg ggacatcagc cacagcaagg cgttcgcgga tggctgcgaa gagcaccatc
     2521 accagcgaaa aaccgacagc ggcggaaaaa ccgtacagcg ccgactgcaa gaaattgtgc
     2581 ccgagattga tattcagcaa cgccacgccg agcacggcac agttggtggt gataagcggc
     2641 aaaaaaatcc ccagcaaacg gtaaagcacc gggctggttt tgcgcaccac catctcggtg
     2701 aactgcacga ccacagcaat caccagaata aatgccaggg tgcgcaggta aatcagatta
     2761 agtgggatca aaatccacgt atcgataagc caggcgcaaa tagacgccag cgtcatcaca
     2821 aacgttgttg ccagccccat gcccatcgcg gtttccagct ttttggaaac tcccataaac
     2881 ggacagagac cgagaaactt gaccagtaca aagttattga ccagtacagt tccgacaaac
     2941 agtaacaggt agtcagtcat ttttcggcct gaaataaaaa agccgcctat tatccgttaa
     3001 tccagagcag gcgacaacag gttaactgta aggttattac ggattcacaa acgtcgcttt
     3061 cacccgcgac gagcgtttga aataggggac gatcaatgcg gttgccaaca aagtaaacag
     3121 caactggcga acggctatgc catcttccac gggtgaaaaa gcgaacgctt ttactgccag
     3181 caatacggaa ataagcagcc agataatata gtgtttagga acacaacgcc gacgtttaaa
     3241 gaaagcgatg gtcaaccaca gtgtgtaata ccacatggcg atagcggtaa taaaagaaac
     3301 gccccataag atttgtgtcg tcagtgcttg tgcgccgagc gtttgaaatg tttgcggaga
     3361 agataacgca gcggtataca gcaacaacgc cagcgtcgta ctcaacagag cgactaaaag
     3421 ccaggccaac gggccaagca accagcctcc aatacgttgt ggcgttgtgg tggtcatagg
     3481 ttctccagaa tagcgacgca aacaggtaaa tctaatttta ggggcgttat tataaggctt
     3541 ttataaccga tcgctacttt ctttattgaa catagtgcca gacggacttc ggtacctggc
     3601 cgaggtcaaa caaacgccct ccagaaacca gctctgcgcg acggtgatcg gcagcacgat
     3661 acatattgat cagttcctga tcgtccgtca gggtgtaatt aagatggtcg tagagttttt
     3721 ccagactttc gagtgaactg attttgcgaa attttaataa gtagtcctga acagtcataa
     3781 aaggtccata aaataattaa tacca
//
LOCUS       DAZUCE010000336         3717 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZUCE010000336 DAZUCE010000000
VERSION     DAZUCE010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.338"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..978
                     /locus_tag="ACHY03_004895"
     CDS             <1..978
                     /locus_tag="ACHY03_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFS3908006.1"
                     /translation="YDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRVKQL
                     ARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHIAKK
                     LLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLVARV
                     KVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCVGNS
                     SERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEPEED
                     ADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            complement(1293..1715)
                     /gene="subB"
                     /locus_tag="ACHY03_004896"
     CDS             complement(1293..1715)
                     /gene="subB"
                     /locus_tag="ACHY03_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit B"
                     /protein_id="HFS3908007.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDGMFSGVVIDQFHT
                     GQIDNNPYFCIEGKQPGGSSIRACSMKNSSVWGPSFSTLYNQALYFYTTGQLVRIYYE
                     PGVWTYPPFVKALTSNALVGLSTCATSTECFGPDRKKN"
     gene            complement(1732..2775)
                     /gene="subA"
                     /locus_tag="ACHY03_004897"
     CDS             complement(1732..2775)
                     /gene="subA"
                     /locus_tag="ACHY03_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFS3908008.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWTELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSMNKKRDPVIRV
                     AALAQYRKGETPVLHGGGITGSRFGNGWVDIAAPGQNITFLRPDGKTGTGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEGRVLNAEKAISMFCKKN
                     YIPVRQGRMSEEL"
     gene            complement(3480..>3717)
                     /locus_tag="ACHY03_004898"
     CDS             complement(3480..>3717)
                     /locus_tag="ACHY03_004898"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908009.1"
                     /translation="ISGLFYISPAKVIRHAGYIIRESGSERRYLLPQSYLINPYQQGN
                     SMAKSSNYAPVKVIIYVDIGKDPCHTVIINRSDN"
BASE COUNT         1068 a          827 c          822 g         1000 t
ORIGIN      
        1 tatgatttta atgaaaaaat agatttctgg tatgtgatag ccctcgcagg acagtctaat
       61 ggtatggcct acggtgaagg tattcccctg ccagatacac tcgacaaacc cgagtcccgt
      121 gtaaaacaac tggctcgtcg aaaaacaatc acaccaggag gaaaagagtg taaattcaat
      181 gagattattc cggcagatca ctgtctgcat gatgttcagg atatgagtgg atatcaccat
      241 ccggctgctg atttgcataa aggggaatat ggttgtgtcg gacagggatt gcatatagct
      301 aaaaagttac tgccatacat acctgagcaa gcagggatat tactggttcc ttgttgcaga
      361 ggaggtgccg cctttactgt tggagctgaa ggtatgtatg tacctgatac aggggcaaca
      421 gctgatgcaa tgcggtgggg caccggaaca gcactctatg aggacttagt agcccgggtt
      481 aaggtagctc tggaatataa cagaaagaat aaactgctat ctgtctgctg gatgcagggg
      541 gagtttgatc ttatgtcacc agattatgaa aagcatcctg acttgtttta tcagatggta
      601 acttctttca ggtctgagtt atctgaatac tcatctcagt gtgttggtaa ttcgtcagaa
      661 agagttccat ggttgtgtgg tgatacaacc tggtactgga aggaatctta tcaaaaagaa
      721 tatgatttta tatatggaca ttacagacaa cggactgatg atgaaataca ttttctcagc
      781 tttcaggata gtaaccgaca tgaattaact aatgagccag aagaagatgc cgatgattta
      841 tctgtcgggt atctggggtc atcatggagg acagaattaa gctggacaac gtcacagaga
      901 agtacacact ttaattcaat ggccagaagg ggagttattg ctgaatgtta tgcgcaaaaa
      961 ataagaaatt atttgtagac aagttcagga tatatctaca gctctgacat gattctctga
     1021 gagacatgac ggagtggtat ggagttgtcg gtatcaacac caagatagct ggtcgttacc
     1081 gtttgatatc actggttctg atactatgtg gacggtagtg tgtcacgaag gcacgcacca
     1141 gggacgtgag tccctgtaat atcatcgaca gtcccattat ggcatgtctg cacctacaac
     1201 gtcatttcga ggtcggctca gtgttcactc gagtcttata tcactgacgc cgacactcca
     1261 tccagagtat ctctggacgg aaacatcacc tattagttct tttttctgtc agggccaaaa
     1321 cattcagtcg atgtggcgca ggtcgataac ccaacaaggg cattggacgt taatgccttc
     1381 acaaagggcg gatacgtcca taccccgggt tcataataaa tcctgaccaa ctgacctgtt
     1441 gtgtaaaaat atagtgcctg attatataat gtggaaaacg aaggccccca gacagatgaa
     1501 ttcttcatcg agcaggctct tatggaagaa ccaccaggtt gtttcccttc aatacaaaaa
     1561 taagggttat tatctatttg ccctgtatga aactgatcaa taacaacgcc tgaaaacatg
     1621 ccatcccggg catcgccagt ccactcagcc atggcaggat taagggataa acatcccata
     1681 acacctgcac atacaaaaaa cctcttaatc gtcatattca cctccggaat tttacagttc
     1741 ttcactcatc cttccctgac ggacaggaat ataatttttc ttgcagaaca tgctaattgc
     1801 tttttctgca ttaagaaccc tcccttccgt caccttatct gcaagtgagg ggtatttgtc
     1861 tgcagactcc agcagtgtac gcttcagttc tgtcgctgtt gcacggggat tacatgagac
     1921 catcgctgcc agaacgccgg aaactatagc tgttgcttcc gaggttccac tacctgtccc
     1981 cgtttttcca tctggtctga ggaatgtaat attctgtccc ggtgcagcaa tatcaaccca
     2041 gccgtttccg aaccgacttc cggtgatccc tccaccatgc aatacaggtg tttctccttt
     2101 ccggtactgt gccagagctg ccactcttat cacaggatcc cgctttttat tcatactgct
     2161 gaccggatgg taagccgcag gccatattct ctgctgagca ctcagttttc gtatatctgc
     2221 gccatcatta cccactgccg caacaattaa tcgctcatta ttccggccca tacgggaaag
     2281 cagttcggtc cagaccgatg cggaagatat ccccttctgg cctcccgata tattaatgat
     2341 tttctcttct cctggtgcaa gaaaaacatt tgacataatg ctttcagttg ctctaatcca
     2401 tgagtcctgt acaccgtcag gaataaccct tctgctggat atcagtgcat gaggataaac
     2461 accataaacc ccatgacgtg aggcaatgag tgaagccatg gcagtaccat gaatatataa
     2521 agcttcagac tcttttaccg ggaatggcga accatcctga gtaaaactga atttggcaaa
     2581 ttcgctgtcg ctgaggcctc ctacaaatgc tactccggaa tcaacaacag atacgacaac
     2641 aggggtattc ttgtctgtga gcgacatggt tgtttctgtc aggccaattg catcaaaata
     2701 ccagggtttt tctgccctga cagacacaga tataaaaagc aggaacagaa tatgcggcca
     2761 taaaatctta agcacaatat cctccagtca gtacaaataa acagtattgc aggtcaatat
     2821 cttacaggaa catcacatct gaaatatcct gttttcgaca aaagataaac aatcacaacc
     2881 aataaagcga gtgaagcccg tctcaaaaac attaattttt atatttatgt atccaatgaa
     2941 ttaacgctca ccttcacggt aatatgcatc tggatttctg caacaggtca gcgataagat
     3001 gctctgcctc ctccttcaga gcagaagtaa atacagccat gatggataaa acttaaccag
     3061 aaaaataaga ggaggcaagt aagttgcctg gcatgtttat gacaataaaa agttccgtca
     3121 ggcatcatgg cgaacaggat atcacagcgc cgttttaccg gggcaatcag tgaatggtgg
     3181 tgtggttcat tggtaagccc cggatatcag gtcactgagt atattcatga atgaccgtta
     3241 aaccggggat gcgagctgct ccccgggaaa gtcgaactga ctggtcaata gatatcaata
     3301 cggggcagtc agtccactta caggtatctg acaaggacac cttctgagcg tgcaatgtgg
     3361 acgggtaata caacgatggt agcaggctgg tcagcaatca gcagtatatg cccatatttc
     3421 ctgaggccgg atatctgttt cctgagcttg ttttcgacgt ttgctaatat tttgtcgaac
     3481 taattatcag aacgattaat gataacagtg tggcagggat ctttaccgat atcaacgtaa
     3541 ataatgacct taacaggtgc gtaattgctg gattttgcca tgctgttacc ctgttgatac
     3601 gggttaatta aataactttg gggaagcagg taccggcgtt cactaccgct ctccctgata
     3661 atgtatcctg catgcctgat aacttttgcc gggctaatgt aaaataaccc tgaaata
//
LOCUS       DAZUCE010000337         3695 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZUCE010000337 DAZUCE010000000
VERSION     DAZUCE010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.339"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(91..783)
                     /locus_tag="ACHY03_004899"
     CDS             complement(91..783)
                     /locus_tag="ACHY03_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFS3908010.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQSGKPAVNHSKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSSWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            894..1121
                     /locus_tag="ACHY03_004900"
     CDS             894..1121
                     /locus_tag="ACHY03_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908011.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            1152..1691
                     /locus_tag="ACHY03_004901"
     CDS             1152..1691
                     /locus_tag="ACHY03_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFS3908012.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAEDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGHLVEQDSFMARLAEMEKELCEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            1688..2707
                     /locus_tag="ACHY03_004902"
     CDS             1688..2707
                     /locus_tag="ACHY03_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS3908013.1"
                     /translation="MTGSKMAKVGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     VAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVISEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVEK
                     PDAAIQSGSRWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            2704..3405
                     /locus_tag="ACHY03_004903"
     CDS             2704..3405
                     /locus_tag="ACHY03_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFS3908014.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVTAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGATV"
     gene            3402..3572
                     /locus_tag="ACHY03_004904"
                     /pseudo
     CDS             3402..3572
                     /locus_tag="ACHY03_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
BASE COUNT         1027 a          858 c          933 g          877 t
ORIGIN      
        1 ttgaaacatc cattctataa attatgagga tgtcagcatg tctcaccaaa acatcatctg
       61 gttatcttga tgaaatctgt cattaataat ctacccatgt tttctatatg tctgtggcat
      121 gctcccaata actttcccaa atataaatac ccgattcatt tcgtcttttt cgatcgggtc
      181 ccacgatgag tagctcttgt tatcagagat aaccagcagc ttatccttca tcatttgcag
      241 gcgctttaca tgggctgtgt cgtcgtacag aaacgcatag ataccatcac cgtcgaaaga
      301 tttaacagtg atatcaacga acagaagatc acctggttcg atcgttcctg acatgctgtc
      361 accgcgtacg ttaatgatgc ggatattttc tgccttcctg ccatcgaaca tgtgacgagc
      421 atcgtcaaac gagtactcaa ccgagcgtag gacttctaca aactcacggt tgatgacacc
      481 cggcccagca ctgacttcta tatcaagaac gtcaatctta aagtatttgg aatggttgac
      541 agcaggcttc cctgattgtt gaccgtcatt tctcatcggg cctatgcctg atgagagcca
      601 ctctgttcga acacccaatg cattagctat ttcaacaatt tttgttgagc cgcgtgcgtt
      661 gccgcttgtc agtctccaga ttgtgggttg agctacgcca gacgcctttg caagagcgcc
      721 ttgagacatt ccagattgtt ccatcgctag gtttaagcga tcagcaagag tttctttttt
      781 cataagtttt aatttatacg cttgcgtatt gatggtcaaa acacgttttg ctattgattg
      841 gattaatacg cattgctatt atctattcat tgtaatacca ataggaattg ataatgacaa
      901 atcaaaccat tcaactcgca atcagtatta caggtagtca aaaacgactg gcagatctat
      961 gcggtgtagc ccagcccact gtttggcgtt ggctacacgg tggcggaatt gatgcccgct
     1021 atgtaatgaa aattgtctca gccactggtg gaaagattaa accagcagat attcgtcccg
     1081 accttgcacc attgtttaac gcgagtaatt ctgccgccta aactgcggcg ttaactgata
     1141 aggcaatgac tatgcaacca cttacatacc aacagactag cggatttagc ccgactgcgg
     1201 tgataaatcg ttctcaaaca aaacaggtgc caggccacga aaaaatccgt gatgccgtcc
     1261 gcgcctggtc ggctgaagat aatcaggatg tcgttgccac actcattgtg aatgagtatc
     1321 gggagcaggg cggcggcact atcgatttcc ctgatgatgt cagccgtgca cgccagaagc
     1381 tgttccgctt cctcgataac aaattcgatt ctgaaaaata ccgaaataac gtgcgtgaac
     1441 tgaccccggc aattctggcg gtactaccgc tggaatatcg cggccacctg gttgagcagg
     1501 atagcttcat ggctcggctg gctgaaatgg aaaaggaact ctgtgaggca aagcaggcgg
     1561 tcattctcaa cgcaccacgc caccagaaac tgaaggagat gagtgaaggc attgtgtcga
     1621 tgtttcgagt ggacccggat ctggctggtc cattgatggc gatggtcacc accatgctgg
     1681 gggcaatatg acaggttcaa aaatggcgaa agtcggtctg cgggaacaga accgactttc
     1741 aggtgcaaat cgtaacacac tcattgcggg aggaattatg gcaaacactg ctgagatatt
     1801 caattttcct gtgccggatg tggcacaaaa ggagccgcgc gtggcagatc tcgatgatgg
     1861 ttatacgcgc attgcaaatg agttgctgga agctgtgatg ctggccggat taacacagca
     1921 ccagcttctg gtcttcctgg ctgtcatgcg caaaacatat ggctttaata aaaaactgga
     1981 ttgggtgagc aacgagcaac tttccgagtt gaccgggata ttgccgcaca agtgttctgc
     2041 tgcaaaaagt gttctggtaa agcgtgggat ttttattcag agcgggcgga atatcggcat
     2101 taataatgtg atcagtgaat ggtcaacatt acccgaatca ggtaagaaaa ataaagttta
     2161 cctgaaagag gtaaatttac ctgaatcagg taagaaaagt ttacccaaat caggtaaagg
     2221 cgtttacccg aatcaggtaa acacaaaaga caaactaaca aaagacaata taaaaccttt
     2281 ttcgtccgag aattctggcg aatcctctga ccaaccagaa aacgatcttc ctgtggagaa
     2341 accagatgct gcaattcaga gcggcagcag gtgggggaca gcagaagacc tgaccgccgc
     2401 agagtggatg tttgacatgg tgaagaccat cgcgccatca gccagaaaac cgaattttgc
     2461 agggtgggct aacgatatcc gcctgatgcg tgaacgtgac ggacgtaacc accgcgacat
     2521 gtgcgtgctg ttccgctggg catgccagga caacttctgg tccggtaacg tgctaagtcc
     2581 ggccaaactc cgcgacaagt ggacccaact cgaaatcaac cgtaacaagc aacaggcagg
     2641 cgtgacagct agcaaaccaa aactcgacct gacaaacaca gactggattt acggggtgga
     2701 tctatgaaaa acatcgccgc acagatgatt aactttgacc gtgagcagat gcgtcggatc
     2761 gccaacaaca tgccggaaca gtacgacgaa aagccgcagg tacagcaggt agcgcagatc
     2821 atcaatggtg tgttcagcca gttactggca actttcccgg cgagcctggc taaccgggac
     2881 cagaacgaac tgaacgaaat ccgccgccag tgggttctgg ctttccggga aaacgggatc
     2941 accacaatgg aacaggttaa cgcaggaatg cgcgtagccc gtcggcagaa tcgaccattc
     3001 ctgccatcac ccgggcagtt tgttgcctgg tgccgggaag aagcatccgt tactgccggg
     3061 ctgccaaacg ccagcgagct ggttgatatg gtttacgagt attgccggaa gcgtggcctg
     3121 tatccggatg cagagtctta tccatggaaa tcaaacgcgc actactggtt ggttaccaac
     3181 ctgtatcaga acatgcgggc caatgcgctg actgatgcgg aattacggcg caaggctgcc
     3241 gatgaactga cttgtatgac cgcgcgaatt aaccgtggtg aggcgatacc tgaaccagta
     3301 aaacaacttc ctgtcatggg cggtagacct ctaaatcgtg cacaggctct ggcgaagatc
     3361 gcagaaatca aagcgaagtt cggactgaaa ggagcaactg tatgacgggc aaagaggcaa
     3421 ttattcatta tctggagacg cacaagagct tctgtgcgcc ggacgttgct gcgacaacag
     3481 gtgtgacatt aaccagcata aatcaggctg cggcaaaaat ggcgcgggca ggaatcctgg
     3541 tcattgatgg taaggtctgg cgaacgtttg tttaacggtt agctactcag gatgataggg
     3601 cggggcaagt gagtatgaag cggattttca ggaatgccgt cagagtttgg aaataaagtg
     3661 ggttttctag tggcaagaga cttgataata tttag
//
LOCUS       DAZUCE010000338         3677 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZUCE010000338 DAZUCE010000000
VERSION     DAZUCE010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.340"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..3117
                     /locus_tag="ACHY03_004905"
     CDS             <1..3117
                     /locus_tag="ACHY03_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS3908015.1"
                     /translation="SGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKG
                     RLAAETSRSGETTSYSYDDPASELPTGIEDATGSTKQMAWSRYGQLLAFTDCSGYTTR
                     YEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTATISPD
                     GKRSTIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQ
                     RGFDGRTQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGW
                     LTTLSHTSEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATR
                     QEPDGLPPVEWLTYGSGYLAGMKLGGMPLVEYTRDRLHRETARSFGGEAYELATAWNT
                     SGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDI
                     DIPYATDPAGNRLPDPELHPDSTFTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEG
                     VIRMHDERTHHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGW
                     MSLSRKPEVTWYGWDGDRLTTIQTGTTRIQTVYEPGSFTPLLRIETENGEQAKARHRS
                     LAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAAQMAAQLEAE
                     YIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQ
                     QYDEESGLYYNRNRYYDPLQGRYITQDPIGLKGGWNLYAYPLNPVQRGDPLGLSGETL
                     VLGSATTVTGTEFGGALLSGGAATFDAVTAAMASAAPALGAMAVVAGPMGYDLLANDG
                     RAGKALSDGQLINSQAEMCYWTSSCPLDSPHENDNSSGSQPNVGKDLTDEEKNCLAGG
                     STGSPGGWGPEDENSARDRMQHERYKDELRQKMGKPDVTDSNLKNIIDDLYRPNAKVG
                     SGSTADAVRYELATGEKVGGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQD
                     LQNALGGK"
     gene            3117..3494
                     /locus_tag="ACHY03_004906"
     CDS             3117..3494
                     /locus_tag="ACHY03_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908016.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DVSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          918 a          844 c         1220 g          695 t
ORIGIN      
        1 tcgggggcgg tgacggcggt gacggggccg gacggcagga cggtacggta tggctataac
       61 agccagcggc aggtgacgtc ggtgacgtac ccggacgggc ttcgcagcag ccgggagtat
      121 gatgagaagg gaaggctggc ggcggagacc tcacgcagcg gtgagacgac gagttacagc
      181 tatgatgacc cggcgagtga gctgccgaca gggatagagg atgcgacggg cagtacaaaa
      241 cagatggcat ggagccgtta cggtcaactg ctggccttta cggactgctc ggggtacacg
      301 acgcggtatg agtacgaccg gtacggtcaa caaatcgccg ttcaccggga ggaaggcatc
      361 agcacttaca gcagttataa cccgcgtggg caactggtca gtcagaagga tgcgcagggg
      421 cgggagaccc gttatgagta cagcgccgca ggcgacctga cagcaacgat atcaccggac
      481 ggtaaacgca gcaccatcga atatgataaa cgcgggcgtc cggtgagtgt gacggaaggc
      541 ggtcttacgc gcagcatggg gtatgacgct gccgggcgca tcaccgtgct gaccaacgag
      601 aacggcagcc agtccacgtt ccggtatgac ccggtggaca ggctgactga acagcgcggt
      661 tttgacggcc ggacgcaacg ttaccactat gacctgaccg gaaaactcac gcagagtgaa
      721 gacgaggggc ttatcaccct ctggcactac gatgcgtcgg accgcatcac gcaccggacg
      781 gtgaacggcg acccggcaga gcagtggcag tatgatgagc acgggtggtt aaccaccctc
      841 agccatacca gtgaaggcca ccgcgtgtcg gtccactacg gctatgacga taaaggccgc
      901 ctgacgggtg aacggcagac ggtggagaac ccggagacgg gggagatgct gtgggagcat
      961 gagacggggc atgcgtacag cgaacagggg ctggcgaccc gtcaggagcc ggacggtctg
     1021 ccgccggtgg agtggctgac gtatggcagc ggttatcttg cggggatgaa gctgggcgga
     1081 atgccactgg tcgagtacac gcgggaccgg ctgcaccgtg agacggcccg cagcttcggc
     1141 ggggaggcat atgaacttgc caccgcctgg aataccagcg gccagctccg gagcaggcac
     1201 ctgaaccttc cgcagcttga ccgtgactac gactggaacg acaacggaca gctaatccgc
     1261 atcagcggcc cgcaggagag ccgggagtac cgttacagtg acacgggaag gctgacgggc
     1321 gtccacacca cggcagcgaa cctggatatc gatatcccgt atgcgacgga cccggcagga
     1381 aaccggctgc cggacccgga actgcacccg gacagtacct tcacggcgtg gccggataac
     1441 cgcatcgcgg aagatgcgca ctatgtctat cgctacgatg aatacggcag gctggcggag
     1501 aagacggacc gcatcccgga aggggtgata cggatgcacg acgagcggac ccaccactac
     1561 gacagccagc accgtctggt gttctacacg cggatacagc acggtgagcc gcaggtggag
     1621 agccgctacc tctatgaccc gctgggccgc cggacgggaa aacgggtgtg gcggcgggag
     1681 cgtgacctga cggggtggat gtcgctgtcg cgtaaaccgg aggtgacatg gtacgggtgg
     1741 gacggtgaca ggctgacgac gatacagacc ggcaccacgc gtatccagac ggtatacgag
     1801 ccggggagct tcacgccgct cctgagaatc gaaacagaga atggcgaaca ggcgaaggcg
     1861 cggcaccgga gcctggcgga ggtgttgcag gaggacacgg gtgtgacgct accggcggag
     1921 ctgtcggtga tgctggggag gctggagcgg gaactgcgcg cgggagcggt gagtgcggag
     1981 agtgaggcat ggcttgcgca gtgcgggctg acggcggcgc agatggccgc gcaactggag
     2041 gcggaataca tcccggagag gaaacttcat ctttaccact gtgaccaccg tggcctgccg
     2101 ctggcgctta tcagcccgga aggggaaacg gcgtggcagg gggagtatga cgagtgggga
     2161 aacctgctgg gcgaaaccag tgcgcagcac cttcaacagt cactccgtct gccggggcag
     2221 cagtatgatg aggagtcggg gctgtactat aaccggaacc ggtactacga tccgttgcag
     2281 ggacgctata tcacccagga tcccattggg ctgaaggggg gatggaacct gtatgcgtat
     2341 ccgctgaacc cggtgcagcg gggggatcct cttgggttgt ctggtgagac actggttctt
     2401 ggttctgcca caacagtaac aggaacagag tttggggggg cgttactttc gggtggggcg
     2461 gcgacttttg atgcagtcac ggcggctatg gcgtctgctg cccctgctct tggagcaatg
     2521 gctgtggtcg ctgggccaat gggatatgac cttctcgcta atgatgggcg tgccgggaag
     2581 gctctgagcg atgggcagtt aataaattct caggctgaaa tgtgttactg gaccagtagt
     2641 tgtcctttag atagcccaca tgagaatgat aattcgtcag ggagtcagcc gaatgttggg
     2701 aaggatttga cggatgaaga gaagaactgc ctggccggcg gcagtacggg aagcccggga
     2761 ggatggggac cagaagatga gaatagcgca agggatcgta tgcagcatga acggtataaa
     2821 gatgaattgc gacagaaaat gggaaaaccc gacgttacag attcaaattt gaaaaatatt
     2881 atagacgatc tttatcggcc aaatgcaaaa gtaggaagtg gaagcacagc agatgctgtt
     2941 cgttatgaat tggccacagg tgaaaaagtt ggtggtagag gacatgtatt gaaagcacag
     3001 gattatacaa gggctttaca ggattggttg gataagaatc caacagcatc tccaggggat
     3061 cgagcagcag cagaaaacgt tcttcaggat ttacagaatg ctttgggagg caagtaatga
     3121 accaatatat tgctagttat atagaaaaaa tggcatcatt ttccactgtt ttgtctgaga
     3181 cagtctcatt agctaatgaa tactggcatc cagaaacgcc accggtggtt atgttgttct
     3241 ctcaggttgg aaaaacgatt gtaaaaaatt tttatgaaat gggggatatt gaaagagaaa
     3301 aaatatttaa acatgtcgaa gatggtatgg tctcgcttga tgatgaattg gccacagttg
     3361 tagctacagg gcttatagag gcaatagtca ctgcgaccga tagcaattct ttactttggc
     3421 aagatgtaag taaatttctg ggtgacaaat caaagtctca tgcattagtg tggaaaaatt
     3481 ttggtcagga gtgatattat tcaagaagta ttaagctcgc tattgagatc ttaatgcttc
     3541 ttgagattaa attaaagtta ggacgaagta attgcaggtg gaaataaatc catggtgacg
     3601 tgtggcaatg gctattagat gttatggtgt gatgggtttt ggtgatttaa gagctaagtt
     3661 agattgcctt atgaaaa
//
LOCUS       DAZUCE010000339         3536 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZUCE010000339 DAZUCE010000000
VERSION     DAZUCE010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.341"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..508
                     /locus_tag="ACHY03_004907"
     CDS             <1..508
                     /locus_tag="ACHY03_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFS3908017.1"
                     /translation="TEPVDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQ
                     PVVTLAQKGKYTVIDGFHRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIR
                     HNRARGKHQIDALSDIVRDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEEN
                     FSPAWTIK"
     gene            complement(638..1561)
                     /locus_tag="ACHY03_004908"
                     /pseudo
     CDS             complement(638..1561)
                     /locus_tag="ACHY03_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1662..3407)
                     /locus_tag="ACHY03_004909"
     CDS             complement(1662..3407)
                     /locus_tag="ACHY03_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS3908018.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLVQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
BASE COUNT          966 a          888 c          767 g          915 t
ORIGIN      
        1 cacagaacct gttgattgtg ttatatggat aaaaaacaat ctaattgaag caaacgacta
       61 taatcctaac gtcatggctc cctccgaaaa aaggttatta acacactccc tgttggttga
      121 tggattcact caacctgttg tcaccttagc tcaaaagggg aaatacaccg ttattgacgg
      181 atttcacaga caaatgcttg ggaaaagcac aacaaaacta gagaaaaaac taaaaggata
      241 tttacctgtc acatgcatta agcatgataa taactttaaa gcttactcta tcgcctcgac
      301 catcaggcat aacagggcca gaggaaagca ccagatagac gcgctctccg atatcgtccg
      361 agatctttcc cgaatgggat gggatgatct ccgaataggt agtgaactgg gcatggatac
      421 agatgaggta ctgagattaa aacaaataag tgggttaaca gaactatttg aggaggaaaa
      481 ctttagtccg gcatggacaa taaagtaatc atttaggggg tgactttatc cagtaattac
      541 cgactgatat atgatcaccc catgtaatgg acagctattg acagatcaaa aacatggggg
      601 atattaattc aactctgaat actcgctaat gtagtggtca agtaatattg gccacggttt
      661 tacagtaaaa atggtatctg ttctctgact cttccggcgt cagcccaccg ttataatggt
      721 gaggtctgac gctgttgtag tagttcaata tgtaatcatt aatttgctgc cgggcctcgt
      781 ccttgcctgc gtaaccattc gttggcaccc attctgtttt cagactgcgg aagaagcgtt
      841 ccatggggct gttatcccag cagtttcccc gccgactgac gctcaagcag attcggcagg
      901 ctggtatgct cctgacgggc atttttatac tgatgttttc cgggctgaca actgctcagg
      961 ttcagatatt tcatcagacg tccggcacgg taacggctca tcgggacgcc gttttgagtc
     1021 agcatttcag ccagagtgcg cgcgcccgca gagccccgac tttggttcca cgcccggcgt
     1081 atttcgctgc acaacctgac tcgtgccgga ttaaccgtat cgcgtcgttt tcgccagtac
     1141 cggtaactgc tgcggtgtat ttccagagca gaacacaggc tgacaaccgt gtggctgtca
     1201 cttagtctgg cggctatcgt gaaccgttca gcgagtcgga cattaagagc gcggtagcct
     1261 tttttaatat cgtattttgt tcctccagac ggcgaacttg cttttccagc tcgcggatac
     1321 gttgctggtc tggagtaatg ggtgtggcag agggcgtaat accctggcgc tctcgcctga
     1381 gctggcgtac ccagctctca agcgtggttg aaccgacatt catcgcttca ctggcctgtc
     1441 gatatgagta gcccttatca acaatcagct gtgcacattc cagcctgaac tcaggggtga
     1501 aggttcgttt ggttttcttg ttcattaagt cacctgtttt gtgttgtggt gagaatatca
     1561 cctttcatca ggtggccaaa tttagtgtgc cactacacta aatacccatc ttagattatc
     1621 aaaaaacgaa tgattacata tcagcaataa taaaacataa cttatacaaa atcctcaaga
     1681 tgacgactga tactgttttc acgataacag gtcaccagta attcccgtgt agcccgggtg
     1741 aacgcaacat aaagcgccgg aataacttca gactcatccg cagcacctga gtgaacgaaa
     1801 gaagcattga taaccgcaac atacggaaac tccagtccct tactgctctg gtaggtcagt
     1861 aaatgcacaa catccttgcg tcgcgaatat ttcttcttac cttcactgtc aaagcaggtg
     1921 gcataaggta tcccgcgttg tgtcatgacc tctttcagat gtttcactga aaactctgca
     1981 ggacacaaaa cagccatatc actccagtga ccgcacagtg tatagcgttt ctccagccag
     2041 cccacaacct gcactgcctc atcactctca gaagcacaac gcagaatttc cggctctgtg
     2101 ccctcttcac cgcccgcctg tggctgaaca aacggtattt cacggttctg atgcttctca
     2161 aaatactcac gggagaatgc ataagcaaag tgcagaatgc gtttcgtatt acggtaattt
     2221 accggcagga cggaagtacg tccctgcgcc tgaatcccca cactggcaag cgaaaagttc
     2281 agagcccgtt cccgtctgta aatggactgt gcgtcatcgt acatcaggag cagtgaacgg
     2341 gatgcgttat caaacaggcg ggctatcaga gccagccagc gactctcaaa atcatgcccc
     2401 tcatccacaa gtacggcatc gtatccagta tcagtaattg ttccgctgtt cacggcatct
     2461 tccagcgcac tgaagcattt ctccgggtaa tcctttcctc cagcagtcac ctgaattcca
     2521 tggcgcttta tcattgaggc gcaccaacta tgaaaatgga ataccgtaac ccgtgacttc
     2581 agtcccctgg actcaataca ttcccgcagg tagctggcca gagtaatgtt gtaacatagc
     2641 accagaatct taccaggggt agtttcagcc aagtaaagac aacggaacaa aagaatcatt
     2701 gtttttccag agccggctac cccatgaata acacgatgcc catcaccaat atttctggcc
     2761 agaatctcct gctgaatatc cattattttg atttgtgagt gctgtctgac ggtcatttca
     2821 ggaaacagat gagcacgcag aatatctcta acctgtggtg tcacccgggt tctgaatccc
     2881 atcgtaaaca tcccggcaat tttttggcgg aatatatcag gtaaaacaga ctgagtcata
     2941 tcatcctgac agattgtctg cgctgaaggg aaaatccttt ccacagcatt ttcatcattc
     3001 ccagccagag ccttcagttg ctggcgggta atacgggtaa acacaacacc atacgcccag
     3061 gccagattca gacgcccctt atactgacca tcattctgtc gcaaacgtga gtctgcaggt
     3121 aaagcattta cggtatcaca ggcataacga cgaacctgta ccagcggatt gatttctgat
     3181 ttaagaatac cgttagtttc aagaatgacc tgttcgtgat ttacctgatg caacgtgtga
     3241 acggtccagt ccttgacttc aagaaatacc agaccattct ccgggtcaat aatgacaaaa
     3301 tcaggatgtc ggtttttccg tcccaccgga atatcgtacc agaccagaca ctcattactc
     3361 ataaatgcct caagacggga tgcaacccgc ttttccccgg cagtcatccg ctcctcggta
     3421 acacctgata tatttatcag tttagccata aaacagcccc tgacagatga tgaacattta
     3481 atatataaaa cttatcatat attgtagcac tagtatatcc gttgcttata agcccc
//
LOCUS       DAZUCE010000340         3532 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZUCE010000340 DAZUCE010000000
VERSION     DAZUCE010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.342"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1611
                     /locus_tag="ACHY03_004910"
     CDS             <1..1611
                     /locus_tag="ACHY03_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFS3908019.1"
                     /translation="IIDSVDTEYIEKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLP
                     FAFIIIVLLVVYLLTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIIS
                     IKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEA
                     VEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVN
                     DFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLN
                     EIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKF
                     ITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERF
                     TTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKR
                     FYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKL
                     GCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            1619..2947
                     /locus_tag="ACHY03_004911"
     CDS             1619..2947
                     /locus_tag="ACHY03_004911"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS3908020.1"
                     /translation="MSRINKFVLTVSLLIFIMISAIACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(3017..3346)
                     /locus_tag="ACHY03_004912"
     CDS             complement(3017..3346)
                     /locus_tag="ACHY03_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908021.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(3336..>3532)
                     /locus_tag="ACHY03_004913"
     CDS             complement(3336..>3532)
                     /locus_tag="ACHY03_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3908022.1"
                     /translation="KSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMANAFLAFS
                     NKQIKDLIETGFLIEVKNER"
BASE COUNT          962 a          702 c          816 g         1052 t
ORIGIN      
        1 attattgatt ctgtcgatac tgaatatatc gaaaaaacat cagcggtgcc aaataacgca
       61 ttattcgaaa tctattttta ttatccgggc ggtaatttat tgaacgcatc agataaactt
      121 ttttatctgc cgtttgcgtt cattattatc gtattgctgg tggtttattt actgaccact
      181 cgtgtgttcc gtcggcaatt ttctgaaatg acagagctgg ttaatacgct ggcgtttttg
      241 cctgactcaa cggatcagat cgaggctctg aaaattcgcg aaggcgatgc gaaagagatt
      301 atcagcatca aaaattcgat cgcggaaatg aaagatgccg aaattgaacg gtcaaataaa
      361 ttgctctcac tgatctctta cgatcaggaa agtggtttta ttaaaaatat ggcgattatt
      421 gagtccaaca ataatcagta tctggctgtg gggatcatca aactgtgtgg tctggaagcc
      481 gtggaagcgg tgtttggtgt tgatgaacgc aataaaatcg tcaggaaatt gtgtcagcga
      541 attgccgaga aatatgcaca atgctgcgat atcgtgacat tcaatgccga tctctattta
      601 cttctgtgtc gggaaaatgt acagacattt acccgtaaaa tagcgatggt aaacgatttt
      661 gacagcagct ttggctaccg caatctgcgc atccataagt ctgccatttg tgaacctttg
      721 cagggggaaa acgcctggag ttacgcagaa aaactgaaac tggcgatttc cagtatccgt
      781 gaccatatgt tctccgagtt tattttctgt gatgacgcga aactcaacga aatagaagag
      841 aatatctgga ttgcgcgtaa tattcgccat gcaatggaaa ttggcgaact attcctcgtc
      901 tatcaaccga tcgttgatat taacacccgc gccattctgg gcgcggaggc gttgtgccgt
      961 tgggtgtctg cggagcgggg gatcatttca ccgctgaagt tcattaccat tgctgaagat
     1021 atcgggttta tcaatgagct gggttatcag attattaaaa cggcaatggg tgaattcaga
     1081 cattttagtc agcgtgcatc gctgaaggat gatttcttac tgcatattaa tgtttcgccc
     1141 tggcagttaa acgaaccgca ctttcatgag cgttttacca ccatcatgaa agaaaatggc
     1201 ctgaaggcga acagcctctg tgttgagatc actgaaaccg tgatcgagcg aattaatgaa
     1261 catttttatc tcaatattga acaactgcgt aaacaagggg tacggatatc gattgatgac
     1321 tttggcaccg gtttgtcaaa cctgaaacgt ttttatgaaa ttaatccaga cagcataaag
     1381 gtggactcgc aatttaccgg cgatattttc ggtactgcgg gaaaaattgt gcgcattatt
     1441 ttcgacctgg cacgctataa ccggatcccg gtgattgcgg aaggcgtaga gagcgaagac
     1501 gttgcgcgcg aattaatcaa attaggatgt gttcaggctc aggggtatct gtaccagaaa
     1561 cccatgccat tctccgcctg ggataaaagt ggaaaattag taaaagagta gtttacgtat
     1621 gtccagaatc aataagttcg tacttacagt cagtctgctg atttttatca tgatttcagc
     1681 aattgcctgc gggatctaca ctcaaatggt aaaggaacgg gtgtatagcc tgaaacagtc
     1741 cgttattgat actgcttttg cggtggcaaa tattgctgaa tatcgtcgta gcgtggcaat
     1801 tgatcttatc aacacgctaa atcccacgga ggaacagctg ttggttggct tgcgcacagc
     1861 ttacgccgac tcggtttcac cctcttattt gtacgatgtc ggtccttatc tgatttccag
     1921 tgacgaatgt attcaggtaa aggagttcga gaaaaattat tgtgcagata ttatgcaggt
     1981 tgtgaagtat cgacatgtca aaaatacagg gtttatctct tttgacggta aaaccttcgt
     2041 ctattacctc tatccggtaa ctcacaatcg tagtctgata tttttgcttg gtctggagcg
     2101 tttttcttta ctgtcaaaat cgctggcgat ggacagcgag aacctgatgt tctctctgtt
     2161 taagaacggt aaaccggtga ccggtgatga atataatgct aaaaacgcca tcttcaccgt
     2221 ttcggaagcg atggagcact tcgcctattt gccgaccgga ttgtacgtat ttgcgtataa
     2281 aaaagatgtt tatttgcggg tttgtacatt gattattttc tttgccgcat tggtggcagt
     2341 gatatcgggt accagttgcc tctatctggt acgcagagtg attaatcgtg gtattgtgga
     2401 gaaagaagcc attattaata accattttga acgcgtactg gatggcgggc ttttcttttc
     2461 ggctgccgat gtcaaaaaac tctacagtat gtataactcg gcgttcctgg acgacctgac
     2521 caaagcaatg ggcagaaaat cctttgacga agatttaaaa gcgctgccgg aaaaaggcgg
     2581 ttatttgtgc ctgtttgatg tcgataaatt caaaaacatt aacgacacct tcggtcattt
     2641 gctgggtgat gaagtgttga tgaaagtggt gaaaatcctt aaatcacaga tcccggtaga
     2701 taaaggtaaa gtctaccgct tcggcggtga cgaatttgcg gtgatttata cgggtggaac
     2761 gctggaagag ttgctatcga ttctaaaaga aatcgttcat ttccaggtgg gaagcattaa
     2821 tttaagcacc agtatcggtg tagcgcattc aaatgaatgt actaccgtcg aacgcttgaa
     2881 aatgctggcg gatgagcggc tgtataagag taagaaaaac ggcagggctc agattagctg
     2941 gcagtagaat tagaatagtg ttagtacccc tgaatcaagg ggtactaatg aatgtggtaa
     3001 atcatttgca aacattttac tttgcaaaaa cttccggccc ttttattgcc aatgtattca
     3061 aaatacgtag tgccgggccg ctcggtttta tttcattacg ttcccatttc gagacacttt
     3121 cttttgacat tcccattgta tgtgccagca cggattgtga catgccgtat cgggtgcgga
     3181 tggctttgat ctcttcaccc gtcattgatt tgactttggc tatacgggcg ctgacttcac
     3241 gggctttgac catgacggca atggcatcaa cctcactatc agacatcagg cccagtgcat
     3301 tcagttcctt tgcatcttcc agcatcattt gtaatttatc gctcattttt cacctcaatc
     3361 aaaaagccgg tttcgatcaa atcctttatt tgcttattgc taaaggccag aaatgcattt
     3421 gccatttttt tgtaggccgc cagttcatca tcttcgattt ctttggttcc tttactgctt
     3481 agaccactct tggaccaacc atcataaaaa aacacatgat ttgctgattt aa
//
LOCUS       DAZUCE010000341         3520 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZUCE010000341 DAZUCE010000000
VERSION     DAZUCE010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.343"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..497
                     /locus_tag="ACHY03_004914"
     CDS             <1..497
                     /locus_tag="ACHY03_004914"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908023.1"
                     /translation="YPIDSVEYRNHEKHIPVLQSALTDDIAIRETLLVKNTFWQYEDE
                     KRIFLTKRYLTQSFGRLNFALSNDNSGRIHHHAPPLIAGVIFGPGFDEHKKEPIEFLL
                     RDNRRHSPCAPFYLFDTELTSSGTMKIIRCKEVESSFKTGNSGEISIKDLQKTLIKFG
                     ITKK"
     gene            739..1545
                     /locus_tag="ACHY03_004915"
     CDS             739..1545
                     /locus_tag="ACHY03_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908024.1"
                     /translation="MDINEFPPGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAAVDDDDELLSNKLKQLREIRLAGLEVIHVIHRHGMAEEKTAYEVEAALIDAYP
                     GLTNIMNGAGSNEFGAAHIKELIATYQPETITFQHKTLMISVNRSSKDIDLYDAVRFS
                     WRVSLERARKAEVILATVRGIVRGVYIADEWLKSTRENFPEIPSWDADDEFEATQSSR
                     FGFRGRAAPPEITQLYLGKKIPDDLRKKGAMSPVRYSPGFDFGMELDFRS"
     gene            1626..2390
                     /locus_tag="ACHY03_004916"
     CDS             1626..2390
                     /locus_tag="ACHY03_004916"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908025.1"
                     /translation="MTKRNKTPKFNLDKARKKEIRAALTSVGMTPQHDGWADLFMYAY
                     TDGSNLNGLQLAKALSVHPTLINGLSRGIKLKPEKALSKKRPSKPTLADKRREAKQKV
                     NMGWEALIRTMPMQKPGARKNPETEALWENARRRGTSQASTPAFTPTIIPRKVSAQIN
                     TELPGLQSTQMETPKRYDSVSMTSKVTTKLDNRVLQDFLAKHIPASVVMLSFISTNTS
                     MKPPRPLNWCSGTWMSRKKSKSSEKPRSWPTLFRNW"
     gene            2408..2857
                     /locus_tag="ACHY03_004917"
     CDS             2408..2857
                     /locus_tag="ACHY03_004917"
                     /inference="COORDINATES: protein motif:HMM:NF024783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFS3908026.1"
                     /translation="MSQEVVVVVEEEKPAPALGQPAKLHSTRTAQRIVEQRDATAHMQ
                     FVEWVWNNFAGRCAVTGWQNGERLQAAHIEPATSGNFSADNGLLLTPTIHDLLDAELM
                     AVNPETMTVHFAPGIELGAMFEGKVIEPKLWPLNMAALVKRWKERKF"
     gene            2989..>3520
                     /locus_tag="ACHY03_004918"
     CDS             2989..>3520
                     /locus_tag="ACHY03_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908027.1"
                     /translation="MNGISKIILALTAGFTLSGCVSHTPKNSQPDPAAIEAAKLAQYT
                     DSQLCQAEDNIEPLLNLNPSSHPEFNENYRRAQAIKKVIASRNLSCYCLKMERAEQAT
                     PARPLSPRQKQARASAVCQAYAQRTRFADPTVTFQMCGKGFQSTSAKCKEDLASFERE
                     SQKLRGTARAEYVEISS"
BASE COUNT         1044 a          785 c          834 g          857 t
ORIGIN      
        1 actaccctat tgatagcgtt gaatatagaa accatgaaaa acatataccg gttcttcaaa
       61 gtgctttgac ggatgatatt gcaatcagag aaactctttt agtgaaaaat accttctggc
      121 aatatgagga tgaaaaaaga atttttctca ccaaaaggta tttgacgcaa agttttggca
      181 ggcttaattt tgcattgtct aatgataatt caggacgtat acatcaccat gccccaccat
      241 tgatagctgg tgttattttt ggaccaggct tcgatgaaca taaaaaggaa ccaatcgaat
      301 ttcttcttcg cgataataga agacatagcc cttgcgcccc attttattta tttgacactg
      361 agcttaccag ttctggaact atgaaaataa ttcgatgtaa ggaagttgaa tccagtttca
      421 aaaccggtaa ttcaggtgag atatcaatta aagatttaca aaaaacattg attaaatttg
      481 gtattacaaa aaaatgatgg gttcgtgcgt ttagaaatcg tttctgcgtc caaagccatc
      541 tgccaggaag actggtaaag acagtccgca cttcgctcac agcggacctt cagttgttcg
      601 tccactctgt gccaagagcg gacattgatt attcctctct gtaaaaatct taatgctaac
      661 tagtgggaac tataagttgt caattgacat gattgtgtat attttgcgat gaaaatgtta
      721 aatttcaggt atcgttatat ggatattaat gaatttccac cgggagtaat cgaacatctt
      781 ggctggtatg tctaccgcct gattgacccg agagatggca gtacgttcta cgtagggaaa
      841 gggaaaggta atcgtgtctt tgcccatatg cgtggtgagg ttgctgcagt ggatgatgat
      901 gatgagttac tgagtaacaa actcaaacaa cttcgggaga tcagattagc tggtcttgag
      961 gttatccatg ttatacatcg gcacggtatg gctgaagaaa aaacggctta cgaagttgaa
     1021 gcagctctca tagacgccta tcccggcttg actaacatca tgaacggtgc tggcagtaac
     1081 gagttcggtg ctgcacacat caaagaatta atcgctacgt accaacctga aacaattacg
     1141 ttccaacata agaccctaat gatatcggtt aacaggagct caaaggatat tgacctctat
     1201 gatgctgtac gttttagctg gcgtgtcagc cttgaacgcg ctcgtaaggc tgaggtcata
     1261 ctggcgacag taagaggaat tgtgaggggc gtatatatcg cagatgagtg gctcaagtct
     1321 acccgtgaga atttcccaga gataccctca tgggatgcgg atgatgaatt tgaagctacg
     1381 caaagttccc gctttgggtt ccggggtagg gctgcacctc ctgagattac acagctttat
     1441 ttaggtaaga agatccctga tgatctcaga aaaaaaggtg ctatgtctcc tgtgagatat
     1501 tctcccggat ttgatttcgg aatggaactg gattttagat cgtagccaat atatggcagt
     1561 aatgaatatt tcggggatcg tgtttgctgc ggtcatggtt accattatta gtgtgctgaa
     1621 ctgaaatgac taagcgaaat aaaaccccaa aatttaactt ggacaaagct cgcaaaaaag
     1681 agattcgcgc agccttaacc agcgtgggta tgacccctca acatgatggc tgggcggatt
     1741 tgtttatgta cgcttataca gatggcagca atttaaacgg tttgcaattg gccaaagcct
     1801 tatcagtgca tccaacactg ataaatggtt tatcgcgcgg catcaaatta aaaccagaga
     1861 aagcgctctc caaaaaacga ccatcaaaac ctacgctggc cgataaacgc cgcgaggcca
     1921 aacagaaggt caatatgggc tgggaagcgc tgattcgcac tatgcctatg caaaagccag
     1981 gcgcacgcaa aaaccctgaa acagaagccc tttgggagaa tgcccgtcgc cgtggcacgt
     2041 cccaggccag cacgccagca tttacgccga caatcatccc gcgcaaggtt tccgctcaaa
     2101 tcaacaccga attaccaggc ctgcaatcaa cgcagatgga aacaccgaaa cggtatgatt
     2161 ctgtgagcat gacatcaaaa gttacgacaa agctggataa tcgcgtccta caggattttc
     2221 tggcaaaaca cattccagcc agcgtcgtga tgttgagctt tatatccaca aacaccagca
     2281 tgaagccacc acgaccattg aactggtgca gcggtacctg gatgagcagg aagaaaagca
     2341 aatcatccga gaagccgaga agctggccga cgttgttccg gaactggtag ccgatgatcc
     2401 aatcatcatg tcgcaggaag tagtggtcgt cgtggaggaa gagaaacccg cgcccgcact
     2461 tggccaacct gccaaactcc acagtacccg aaccgcgcag cgcatcgttg agcagcgcga
     2521 cgcgacggcg catatgcagt ttgttgaatg ggtatggaat aattttgctg gccgttgcgc
     2581 ggtgactggc tggcagaacg gcgaacgatt gcaagcggcg cacattgagc cagctacctc
     2641 aggcaatttt agcgctgata atggcctgtt gttgactcca acgatacacg acttactaga
     2701 cgcagagctg atggccgtca accctgaaac aatgaccgta cattttgccc caggtattga
     2761 gctcggcgcg atgtttgaag gcaaagtgat tgagcctaaa ttatggccgc tgaacatggc
     2821 cgcgttggtt aaacgctgga aagaacggaa attctgatta aatattttaa tcaccatcgt
     2881 gctggctgcc atcgcctata tggcgttgcc tcatcactcg aatccgaatg cataatctta
     2941 tccgagtggg gaaagtgcac caccaaaaca tcgaaacagg aatagaaaat gaacggaata
     3001 tcaaaaatca tcttagcact gaccgctgga tttacgctgt ccggatgtgt aagccatacc
     3061 ccaaaaaatt cacaaccaga tccggctgca atcgaggcgg caaaacttgc gcagtacacc
     3121 gattcacagc tttgccaggc agaagataat atagagccat tgctcaacct taatccatca
     3181 tctcatcctg aatttaatga aaattatcgc cgcgcccagg ctattaaaaa ggtgatcgcc
     3241 tctcgtaatt taagctgcta ctgcctgaaa atggagaggg cagaacaggc tacaccagca
     3301 cgcccgttaa gcccacgaca aaaacaagcg agggcatccg ctgtttgcca ggcttacgcc
     3361 cagcgtaccc gatttgcgga tccgacagtc acatttcaga tgtgcgggaa aggattccag
     3421 tctacttcgg caaaatgtaa ggaagatttg gcgagttttg aacgtgagtc tcagaaactt
     3481 agaggtacgg ccagagcgga atatgttgaa attagcagtg
//
LOCUS       DAZUCE010000342         3480 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZUCE010000342 DAZUCE010000000
VERSION     DAZUCE010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.344"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..419
                     /gene="tolA"
                     /locus_tag="ACHY03_004919"
     CDS             <1..419
                     /gene="tolA"
                     /locus_tag="ACHY03_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFS3908028.1"
                     /translation="AVPVLVLILASLTTGCTKDVSTNNYDASLYHSDKLIKSQNLSSS
                     ERSPDASEEIRQYAIQVRKAIEEQLKDASKYSGKECSLRMYMAPNGLLLQVKRESGDP
                     DLCCEAMNAVKNADIPSPPSSGVYKAFRNGVLDFKL"
     gene            886..1050
                     /locus_tag="ACHY03_004920"
                     /pseudo
     CDS             886..1050
                     /locus_tag="ACHY03_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483513.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            1114..1839
                     /locus_tag="ACHY03_004921"
     CDS             1114..1839
                     /locus_tag="ACHY03_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908029.1"
                     /translation="MDVACSVVLIRYPIDIFLEKEKVSLDAGSVMLVARNIRGLFCAY
                     ADRVKMADISNSVVNQYLERVCELTEYTPTKMPLYLMSDIYNTELAEALITQHSESSD
                     TLKDFSVITAFSCISLFATDRRLPLFLSGAVNNISCKVRAIIQTDISAGWMLGVIALQ
                     LHMSESLLKRKLKDEGYSFSRLLLEERMRVAVNLVCFQRECGQAIAEKCGYSSKSYFI
                     SVFHQYYGAPPESYAYLQKADVI"
     gene            2904..>3480
                     /locus_tag="ACHY03_004922"
     CDS             2904..>3480
                     /locus_tag="ACHY03_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFS3908030.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGASA"
BASE COUNT          900 a          688 c          927 g          965 t
ORIGIN      
        1 gtgctgtacc ggttcttgtt ttgatattag ccagtctgac aactggctgc acaaaagatg
       61 tatcgacaaa taattatgat gcttctctgt atcactcaga taaattaata aagtcacaaa
      121 atttgagctc atcggaacgt agccctgatg ccagtgaaga aatccggcag tatgccattc
      181 aggtcaggaa agccatagaa gaacagttaa aggatgcgag taagtattca ggaaaagagt
      241 gctcactgag aatgtatatg gccccgaatg gtcttctgct acaggttaaa agagaaagcg
      301 gtgatcctga tttatgttgt gaagcgatga atgcagtaaa gaatgctgat ataccatcac
      361 caccgtcttc aggagtatat aaagcattta gaaatggcgt gctggatttt aaactctgac
      421 tggggagaag attttctcct gtccagatga ttttggggtg ctggaaagat attgtgctgt
      481 gaagtgttaa attcctcaca attcagtaag ttgacagttg cctgtcagac tgggcatttg
      541 ttaaaaaaat ttcgcatggt gaatccccct gtgcggaggg gcgactggtg aacggtatga
      601 tctctttgat gatcgtaagc gagaatacgc gggtttggtg tcaccgggct gaactcaccg
      661 ggaggcaccc ggcaccatgc tcatggtgat acagaaatgc ggcttcagcc cctctccgga
      721 ggggctttct tatggacaaa aaaagcccgc gctgggagac gcgggcggca aggaataaac
      781 aataaaacgt gaagtaatac ttcaactggc gaataatacc cgacagtaat cactctgcgc
      841 aatagcgtgg cctttttcga attgcgggct gtagtctccc ttctgccggg tgtcctggtt
      901 gcagatttct tcatggggta gggttcggca gttaaagcag cgatggcgct ggggcgtcgt
      961 gcaactggcg ttgagctgga taccgggcgt tttgaacaga ctgcgcagga aatcagggat
     1021 gtatttagcg ggagcggttt gcagaagtaa tctcccaaaa tgccattttt ggaatatact
     1081 ggtattaagt ctttttgctc aggttaattc aaaatggatg ttgcctgctc tgtggtcctg
     1141 attcgctatc cgatagatat ttttcttgaa aaagaaaaag tttcactcga tgccggaagt
     1201 gttatgcttg ttgccagaaa tataagaggg cttttctgtg cttatgccga cagagtgaaa
     1261 atggcagata taagtaacag cgtcgtcaat cagtatctgg aaagggtgtg tgaactgacg
     1321 gagtatacac cgacaaaaat gcctctttat ttgatgtctg atatttacaa tacggaactg
     1381 gcggaggctt taataaccca gcatagtgaa tccagtgata ctttgaagga tttctctgtt
     1441 ataaccgcat tttcatgtat ctccttattt gcaacagaca ggcgtctgcc attatttttg
     1501 tccggtgctg taaataatat cagctgtaaa gtaagggcta taattcagac cgacatatcg
     1561 gcaggctgga tgctaggtgt gattgcatta caattgcata tgagcgaaag cttgttgaag
     1621 cgaaaactta aggatgaagg ttatagcttt agtcggttgt tgctggagga gcgaatgcgt
     1681 gttgctgtta atcttgtatg tttccagcga gagtgtggac aagcgatagc ggaaaaatgt
     1741 ggttactcaa gtaagtccta cttcatttct gtatttcacc agtattatgg tgcaccacct
     1801 gagagctatg catatttgca gaaagcagac gttatctgat gtttttcatc aggcatatat
     1861 tgattgactg tattagatat agtctgaatg tgttcagcat aaaaagttat aatcatttgg
     1921 tgtttgcgtg aatattgatg agaaaatcag atgtagccaa ctgttacagc gtattgaagc
     1981 cgtcaatgtg gaacgaacaa aggtcttcag tcgtctgaca gttttatttt gtacccctga
     2041 tcgacgttca ggtcaggaga tggttcttct ggatgtagat gcacttcgga acgtctgtga
     2101 ggagttcacc gcggctaatt cggagttact gagtcttgtt caggagtaca acagaattgc
     2161 aggtagtaac ggttttgatg aaatcaaaat tatatcccgt ggataaaaca tatcctgtat
     2221 atttatgttg ttacatacag gcgcttttag tatctgccgc acggcagttg tacttcagaa
     2281 taagatgctt ttgtgactgg tggtgttgta tctggggtta gccaggaggc aggttcagag
     2341 agtccggtgg taattatgtg tatgcagtgg ttgttgctgt accgtgggca agttctggaa
     2401 ctggcttcgt ccattgtaga tgattgttag ctggatatca gttacctcat ggaaatgtta
     2461 aatgactcac aattcggttg atggactatt gtctgatcga tggaagtctt gtaaaaacga
     2521 gccagcatag tgaatccccc tgtgcggagg ggtgatatcg tatcatttcc tgtgtggccg
     2581 actgatatca cggacatttg ttcaccggga ggcacccggc accatgcagt atacagagat
     2641 taggcataca tccaggctcc tcatcgcagg agccttttta catgtaaaaa aaaccgctcc
     2701 tgggaagagc ggttggcaag gaataaataa caaaacgtga agtaatttat tcagcaccct
     2761 aataatacct tagagtaatc accttgcgca actgtaaggg cgtatttctt ttttgcgggc
     2821 tgtttttctg tgtgactttt gtgtttccgg aggtcagccc gtgccttctc tgactcagaa
     2881 cattatcccg gccgggagga ttcatggcat ttaaacacta cgatgtggtc agggcggcat
     2941 cgccgtcaga ccttgctgaa cgactgacac aaaaactgaa ggagggctgg cagccatttg
     3001 gcagtccggt ggccatcacg ccttataccc tgatgcaggc cattgcggcg gaaggtgatg
     3061 tcaccacacc agtggcggtg accggtaatg agggtaaggc ggtggctgtc agtgccacca
     3121 gcgacccgga gtattacttt gttgtggttc tggcagggca gtcaaatggc atgtcgtatg
     3181 gtgaaggtct tccgctgccg gagacatatg accgtccgga cccgcgtatt aagcagctgg
     3241 cgcgccgcag tacggtgaca ccgggcggtg tcgcctgtaa atataacgac atcattccgg
     3301 cggaccattg tctgcatgat gtgcaggaca tgagccgtct taaccatccg aaagcggacc
     3361 tgtcaaaggg gcagtacgga accgtggggc aggggctgca tatcgccaaa aaactgctgc
     3421 cgtttatacc ggcgaatgcg ggcattctgc tggttccgtg ctgccgtggt gcttcggcat
//
LOCUS       DAZUCE010000343         3469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZUCE010000343 DAZUCE010000000
VERSION     DAZUCE010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.345"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1659)
                     /locus_tag="ACHY03_004923"
     CDS             complement(<1..1659)
                     /locus_tag="ACHY03_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4851/ECs_5259 family protein"
                     /protein_id="HFS3908031.1"
                     /translation="MLVKTPVNPLLQWLNMFFSRRSLSGADGRALYAYRCTDTEYDSL
                     AELLRTYAPRSYPRTIFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYV
                     HRQKLVNDGIRYWKRKIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQA
                     LRGQSSQYPAAKIAQELGDTIPVTMQNELVYEIAGEFCETLCRLLSEQPPQSSDPVSA
                     LRKLSPDWHLQLPLVLPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDA
                     RFRFPATMRTEQLTSLFECYIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLP
                     IAMQKLSGADAMAEISLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLV
                     GMGSVSSSLNALFISLPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGA
                     VCTIRTQQLYDSAIEYYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWR
                     SIRSGNNAWYSVASEMPKGQIEVRRIVNDEVLFSGKVVVLPADFD"
     gene            1871..>3469
                     /gene="zorA2"
                     /locus_tag="ACHY03_004924"
     CDS             1871..>3469
                     /gene="zorA2"
                     /locus_tag="ACHY03_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorA2"
                     /protein_id="HFS3908032.1"
                     /translation="MLAQLFEQLFQSIDSTLITNIFIWAVIFVFLSAWWCDKKNIHSK
                     FREYAPTLMGALGILGTFIGIIIGLLNFNTESIDTSIPVLLGGLKTAFITSIVGMFFA
                     ILFNGMDAFFFANKRSALAENNPESVTPEHIYHELKEQNQTLTKLVSGINGDSEGSLI
                     AQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQFADLMAKGATEQIIDALRQVIIDF
                     NQNLTEQFGENFKALDASVKKLVEWQGNYKTQIEQMSEQYQQSVESLVETKTAVAGIW
                     EECKEIPLAMSELREVLQVNQHQISELSRHLETFVAIRDKATTVLPEIQNKMAEVGEL
                     LKSGAANVSASLEQTSQQILLNADSMRVALDEGTEGFRQSVTQTQQAFASMAHDVSNS
                     SETLTSTLGETITEMKQSGEEFLKSLESHSKELHRNMEQNTTNVIDMFSKTGEKINHQ
                     LSSNADNMFDSIQTSFDKASAGLTSQVRESIEKFALSINEQLHAFEQATEREMNREMQ
                     SLGNALLSISKGFVGNYEKLIKDYQ"
BASE COUNT         1090 a          722 c          672 g          985 t
ORIGIN      
        1 atcgaagtct gctggtaaaa ctacaacttt accgctaaat aaaacctcat cattaactat
       61 tcgccggact tctatctgtc cttttggcat ttctgatgcg acagaatacc aggcattatt
      121 acctgaacga atggaacgcc aaaaaagttc tttctccggt acaataccat attgcagatc
      181 ctttttccaa ccgatcttcg gccaggctcg atggacagga taatccgatt taaccagttc
      241 aacttctgtc gatttaatat aatattcaat cgcagaatca taaagttgct gggtgcgaat
      301 tgtacaaact gcaccatcat gtaaaacaac gctaaatact ccacttattt tagttaagct
      361 gcgttcactg tttttcagca accttgggat atcaaactca ccttctccac taatatctaa
      421 atggctattt ttcggtagcg aaataaacag agcatttaga ctggaactca cagaccccat
      481 accaacaagt tttagctgcg attcactctc attcatcgct tcaaaaaccc agggaagttc
      541 ttcagtaagt tcataaccgc cttttggaat tgtgtggcct aacagaattg gaccttcatg
      601 aagcgacaat gagatttcgg ccattgcatc tgcaccagag agtttttgca tcgcaatagg
      661 taataactcg acccgccaat cttgctgctc gtaacggctt agcattgcca accttgcccc
      721 gccatttttc cattttcctg aaatgatgag tcgggtctgc tccggctgaa tatagcattc
      781 aaacaaagag gttaactgtt ctgtacgcat tgtggccggg aatcggaacc gggcatcgca
      841 ataccaactg tcatcaacat cgacccagat tctttcaacc tgaagactac ttgcactgcg
      901 tatttcagaa gattgagata gtaatcgcct tacaatctct gcagcattcg cttcggggag
      961 aacgagtgga agttgaagat gccagtctgg agagagctta cgtaatgcag aaacgggatc
     1021 actgctttga ggaggttgct cactaagtaa tcggcagaga gtctcgcaga attcaccggc
     1081 tatttcatag accagttcgt tttgcattgt gacaggaatc gtatcgccta attcttgtgc
     1141 aattttagcg gcaggatatt gagatgattg cccacgaagc gcctgatata tccttctgaa
     1201 ataggtaatc aaataaccgc tctcattttc aatcatgcgg ataggtaaac caccttcaca
     1261 agccagtgta tggagataac ccgaagcttg ccccaggttt cttatctttc ttttccagta
     1321 gcgaatgcca tcattaacca gcttctgtcg atgaacatac ggaactttcc agttaataga
     1381 atctaaaatt gtgtcccatt tagggtgtcc gaccgtatga gtccggcgga taaactctgc
     1441 ggcatatatg ctaaatagaa catcgctata ggaaatgaat atcgttcttg gataactacg
     1501 gggggcatat gtacgcagta gttctgccaa actatcgtac tctgtatcag tacaacggta
     1561 agcatataac gcccgtccat cagctccgga taatgaacgg cgactaaaaa acatgttaag
     1621 ccattgcaaa agtgggttaa ctggagtttt cacaagcaac ctgccttcct ttgatacaat
     1681 tcgtaacagg ttactatcat cataaaaaag ctcaacccga tgaactcgct aaaaatgaga
     1741 caaatcattt atatctcgaa aaaacttgtt acaatcatga gcgctacacc gaacttaacc
     1801 atataaatta tgtgtgtttt gtttattttt taaacgatta caactatcca ttatttacac
     1861 aggtatcaaa atgttagcgc agctttttga gcagttgttt caatcgatag actctacact
     1921 gatcaccaat attttcatct gggctgttat attcgtattt ttatcagcgt ggtggtgtga
     1981 caaaaaaaat atacatagta agtttagaga atatgctcca accttaatgg gggcattagg
     2041 tattctgggt actttcattg gtattattat tggtttactc aattttaata ccgaaagtat
     2101 tgataccagc atccccgtat tattaggtgg cctaaaaaca gcattcatta caagcattgt
     2161 aggtatgttt tttgccattt tatttaatgg aatggatgct ttcttttttg ccaataaacg
     2221 aagtgcgtta gcagaaaata accctgaatc tgttacacct gaacatatct atcatgaatt
     2281 aaaagagcag aaccagactc tgactaaatt agtctcgggt attaacggtg atagtgaagg
     2341 ttctcttatt gctcaaataa aattactacg tactgagatt agcgattcct cgcaggcaca
     2401 attagctaat cacactcatt tcagtaataa gctttgggaa caacttgaac aatttgcaga
     2461 tctaatggca aaaggtgcta cagaacaaat tattgatgct ttgcgacaag tcattattga
     2521 ttttaatcaa aatttaactg aacagtttgg tgaaaacttt aaagctcttg atgcctctgt
     2581 aaaaaaactt gttgagtggc agggaaatta taaaacgcaa attgagcaga tgtcagaaca
     2641 atatcaacaa agtgtcgagt ccctggttga aacaaaaact gcggttgcag ggatttggga
     2701 agaatgtaaa gaaattcctc tggctatgtc tgaactgcgt gaagtgcttc aggtgaacca
     2761 acatcaaatc agcgaactct cccgccattt agaaaccttt gtcgccatcc gcgataaagc
     2821 tacaaccgta ttacctgaaa tacagaacaa aatggctgaa gtgggtgaac tgctgaaatc
     2881 cggagctgca aatgttagtg catctcttga gcaaaccagc cagcaaatac ttcttaatgc
     2941 agattcaatg cgcgttgccc tggatgaagg taccgaagga ttcagacaat cggttaccca
     3001 aacacaacaa gcatttgcct cgatggcaca tgatgtcagc aattcctccg aaaccttaac
     3061 cagcacgtta ggtgaaacaa ttactgaaat gaaacaaagt ggtgaagaat tcctgaaatc
     3121 actagagtcg cactcgaaag aattgcatag aaatatggaa caaaatacga cgaatgtgat
     3181 tgatatgttc agtaagactg gtgaaaagat taaccatcaa ctatccagta atgccgataa
     3241 tatgtttgat tcaatccaga catcatttga taaggcaagt gcagggctga cttctcaagt
     3301 cagagaatca attgaaaaat ttgctctatc catcaacgag cagttacatg cttttgagca
     3361 agcaactgaa cgtgaaatga accgtgaaat gcaatcatta ggtaatgctc tgctttcaat
     3421 cagcaaaggt tttgtcggta actatgaaaa acttattaaa gattaccaa
//
LOCUS       DAZUCE010000344         3465 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZUCE010000344 DAZUCE010000000
VERSION     DAZUCE010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.346"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..158)
                     /locus_tag="ACHY03_004925"
     CDS             complement(<1..158)
                     /locus_tag="ACHY03_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HFS3908033.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALE"
     gene            473..1789
                     /gene="shiA"
                     /locus_tag="ACHY03_004926"
     CDS             473..1789
                     /gene="shiA"
                     /locus_tag="ACHY03_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFS3908034.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            1891..3345
                     /gene="amn"
                     /locus_tag="ACHY03_004927"
     CDS             1891..3345
                     /gene="amn"
                     /locus_tag="ACHY03_004927"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFS3908035.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYIRSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          821 a          843 c          824 g          977 t
ORIGIN      
        1 aattcaagcg cccgaataac aggaaagatg gccatagcat cgccaatctg atcgggaata
       61 tggatgacaa caaagtctgt tttttcaata ttgaaattat aagctttata atcgtagtaa
      121 ctaaatgcaa tacgtctcaa caatgatact aaaaacataa ctaacctcgc ctccctactg
      181 gttataatgc aatgcagtct atcagactca tcagggcgca attttgtgca tatacggact
      241 tttatgtttc atatctcgaa cctgtgggtc ctctgcttaa tccttaaaca acaccagcaa
      301 ctcctgcgct ttcatcttcc atcgaatttt tcatgttgcc gctaatcagc cataaaatca
      361 tttgcagatg cgctctgtcg aggtagtctc ataaggttcg tttatagatc gacggcaatg
      421 tgagttacct tttccatact aattataaaa agacagtaca aacaggatca ttatggactc
      481 cacgctcatc tccactcgtc ccgatgaagg gacgctttcg ttaagtcgcg cccgacgagc
      541 tgcgttaggc agtttcgctg gtgccgtcgt cgactggtat gattttttac tctatggcat
      601 taccgccgca ctggtgttta atcgcgagtt tttcccgcaa gtaagcccgg ctatgggaac
      661 gctcgccgca tttgccacct ttggcgtcgg attccttttc cgtccgctcg gcggtgtcat
      721 tttcggtcac tttggcgacc gactgggacg taagcgcatg ttaatgctga ccgtctggat
      781 gatgggcatc gcgacagcct tgattggtat tcttccttca ttctcgacca ttgggtggtg
      841 ggcacctatt ttgctggtga cactgcgtgc cattcaggga tttgccgtcg gcggcgaatg
      901 gggaggcgcg gcgttgcttt ccgttgaaag tgcaccgaaa aataaaaaag ccttttacag
      961 tagcggtgta caagttggct acggagtagg tttactgctt tcaaccggac tggtttcatt
     1021 gatcagtatg atgacaactg acgaacagtt tttaagctgg ggctggcgca ttcctttcct
     1081 gtttagcatc gtactggtac tgggagcatt gtgggtgcgc aatggcatgg aagagtccgc
     1141 ggaatttgaa caacagcaac attatcaagc tgccgcgaaa aaacgcatcc cggttatcga
     1201 agctctgtta cgacatcccg gtgctttctt gaagattatt gcgctacgac tgtgcgaatt
     1261 gctgacgatg tacatcgtta ctgcctttgc acttaattat tcaacccaga atatggggct
     1321 accgcgcgaa cttttcctta atattggttt gctggtaggt ggattaagct gcctgacaat
     1381 tccctgtttt gcctggcttg ccgatcgttt tggtcgccgc agggtttata tcacaggcgc
     1441 gttaatcgga acgttgagcg catttccttt ctttatggcg cttgaagcac aatctatttt
     1501 ctggatagtt ttcttctcca taatgctggc aaacattgcg catgacatgg tggtgtgtgt
     1561 gcaacaacca atgtttaccg aaatgtttgg tgccagttat cgctatagtg gtgctggagt
     1621 cggttatcag gttgccagtg tggttggcgg tggatttaca ccttttattg ccgctgcgct
     1681 catcacttac tttgccggga actggcatag cgtcgccatt tatttgctgg ctggatgtct
     1741 gatttccgca atgaccgctt tgttgatgaa agacaatcaa cgcgcttgat agcctggcga
     1801 agatcatccg atcttcgcct tacacttttg tttcacattc ctgtgacata ctatcggatg
     1861 tgcggtaatt gtatggaaca ggagacacac atgaataata agggctccgg tctgacccca
     1921 gctcaggcac tggataaact cgacgcgctg tatgagcaat ctgtagtcgc attacgcaac
     1981 gccattggca actatattag aagtggcgaa ttacctgatg aaaacgcccg caaacaaggt
     2041 ctttttgtct atccatcact gaccgtaacc tgggacggta gcacaaccaa tccccccaaa
     2101 acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat tactcgccct
     2161 actctctttc gttcgtatct taatgaacaa cttacgttgc tgtatcagga ttatggtgcg
     2221 catatctcgg tgcaaccctc gcagcatgaa atcccttatc cttatgtcat cgatggctct
     2281 gaattgacac ttgatcgctc aatgagcgct gggttaactc gctatttccc gacaacagaa
     2341 ctggcgcaaa ttggcgatga aactgcagac ggcatttatc atccaactga attctccccg
     2401 ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cgcggttgcg ccattatacc
     2461 ggtacgccag ttgaacattt tcagccgttc gtcttgttta ccaactacac acgttatgtg
     2521 gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc ctacattgcc
     2581 ctttcttgtg ctggcgggaa ctggatcacc gccgaaaccg aagcgccaga agaagccatt
     2641 tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac cgccgatggt
     2701 cagggtatta ctctggtgaa tattggcgtg ggaccgtcaa atgctaaaac catctgcgat
     2761 catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg cggattacgt
     2821 gaaagccagg ccattggcga ttatgtactt gcacatgctt atttacgcga tgatcacgtt
     2881 cttgatgcgg ttctgccgcc cgatattcct attccaagca ttgctgaagt gcaacgtgcg
     2941 ctttatgacg ccaccaagct ggtgagcggc aggcccggtg aggaagtcaa acagcggcta
     3001 cgtactggca ctgtggtaac cacagatgac aggaactggg aattacgtta ctcagcttct
     3061 gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc aaccattgcc
     3121 gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgtgtttc agataaaccg
     3181 ttgcatggcg agattaaact tcccggccag gctaaccgtt tttatgaagg cgctatttcc
     3241 gaacacctgc aaattggcat tcgggcaatc gatttgctgc gcgcagaagg cgaccgactg
     3301 cattcacgta aattacgaac ctttaatgag ccgccgttcc gataataagt tctgaacctt
     3361 cttatcaaaa atagatgtga aggattaagt aagacatctg gcaaatagcc cgcaataaac
     3421 gcgggctgtt tgcttccttg ggcggatacg agttttatta tcgtc
//
LOCUS       DAZUCE010000345         3420 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZUCE010000345 DAZUCE010000000
VERSION     DAZUCE010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.347"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(112..435)
                     /locus_tag="ACHY03_004928"
     CDS             complement(112..435)
                     /locus_tag="ACHY03_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFS3908036.1"
                     /translation="MKRTLLIAVWAIGLMSDSAIALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(443..628)
                     /locus_tag="ACHY03_004929"
     CDS             complement(443..628)
                     /locus_tag="ACHY03_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFS3908037.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(625..3264)
                     /locus_tag="ACHY03_004930"
     CDS             complement(625..3264)
                     /locus_tag="ACHY03_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFS3908038.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNAQLPETGCPSGKECEEKQ"
BASE COUNT          849 a          911 c          780 g          880 t
ORIGIN      
        1 ggaaggatat ataaggtttt tgtagaaata cccacggtaa ttaagcatgc taacgtcagt
       61 ttaaatactg taaaagatca ttaatagata cattttgtag ccaactataa tttatttgcg
      121 caaccattta ccattaactc cctgaacata ttgccccgac ttcgcgcgag caacaagctt
      181 ttgtccagcc agtttggcta tctctttagt ggatacatta ttttgctttg ccagttgttg
      241 gtaactatgg ttacgagctt cattaatatc ttttactaat gcctgtgttt cggcatccgt
      301 ttgtagcgcc actaggtaac cgttaagcgt ttcacctacc cgcccctgac ttctggcttc
      361 gttcagggtt aaagcaattg cactgtcgct catcaggcca atggcccata cagctatgag
      421 taatgttctt ttcatcatca cctcaaaata gatcgctacg ggttttgagc aggtcttcaa
      481 cgtccttatc cgctttaatg atgatttcat gctcaatttt aacgttcata ttaatggtga
      541 ttggctcttt cggcgcagcc acttcaatgc gaggcgtgca accaaccaac ataaaagagg
      601 acgcaaacgc ggctatcact attttcattg tttttcctca cactcttttc ctgatgggca
      661 gccagtttcc ggtagctggg cattctgctc cagccatacc tggagattat cgccaaagcg
      721 taaactgcgc cataacgtaa aaatattctc ttcatggtga taattaaggt ttaccgtacc
      781 gcttttacca tcgacgcgac tggtgcccga aatgttggcc tgcatggtta acacaccgag
      841 attatctaaa ttaatccttg ttgatgagcg actgatttcc atataacgca accagttgat
      901 ggcagttcca gcggtcatat tgtctttggc caccgcatct gcagtgtctt tatccaaacg
      961 cagcgtcatc ggccccgagt ttgccaacca gccatctttg acaatccatt tttcattgtt
     1021 cagccatagt ggcaaggccc cactgaatgc cccagacata gcaaactgtt tcggattaac
     1081 cgcgctaacc agttcgctgg acgatagatt attcaggcgt agcaacgctg ggtcgtgctg
     1141 cggcatacgc aattgtttca ttagtacgtt accgcccaaa acatcaacgc taacatcggt
     1201 cagtagcaat ggttcttctt ctgtccacgg ataacgccct tgcaaatcag cagtgatatt
     1261 tttcgctgtc accaaattaa tgacttcggc aatacgcaaa gtaacaggtc cgcgagtccc
     1321 aagatgccag gcaccatcgg caaaacggaa cggcaataca aaatcgacgc cgttgacctg
     1381 attatctggc atccaggcac taccgccttt caacacgccg tgccctcccg cgcggaatcc
     1441 ttgttcaggc gcagcagaaa atgcaacctg agcatacagt tcaccatcgc gtaagttcat
     1501 cttccagtcg ggtggcacca gcggctggaa tacagtcagt gattgtttag gccaccaggc
     1561 gttaccgcgc agacgaatac cgtcccagcg accatttacc cgaaccgggc caatctcacc
     1621 agcatgtaaa tcgcctttaa agaggaaata cgtcggatcg cgcccatcga cgctaaattt
     1681 taaggttgat ggcggtaaca cactgccgcc agtgaacagc gtttgcccgg cgtccagtga
     1741 cagcgcgccg ctgaaggagg gatgctgtgc gtcacgtacc cagacgacgg gcttgtcgag
     1801 aattaatcgc ggcttttcta ccgtcatcgt accgtattgt aactggtcga aaccggtgga
     1861 gagatcggtc agcgtaatcg tgctgtcatg ccactcacct tttcctgcga catcccaggt
     1921 ggcattcatc ggtgtaaaac tgccttttcc ccagtagcgc cactgccagc ggccagcgtc
     1981 agggagaaaa tcattcgcca gcccatccat atgcagcacg aaatcgccca gttcattttc
     2041 atgcgcctgc aaaatggcct gcaaacgtcc gtcaacacca cgttgggtga cttttacacc
     2101 cgctaaaggc cagcggattt catcgatgtc cagcgaatcg atgactcttc cctttgaacg
     2161 aagtaacgcg cctggctcaa aggtcagcgt tgggtcagac agacttccac ttagctgcgc
     2221 aggtaaacgg gcatataaaa tgaggtccgc ctgtttcgct tcaccggtca gctgcagagg
     2281 cagctggctg ttatccatgc ttaattttcc tgggccaaaa ttaagcaccg cgttgccctt
     2341 acccgcttgc ccctgggtca gcacactcag tcgtccgctg accagagcgt tctcaagccc
     2401 tgcctgccag ttgtcgactt tgacacccag tcggccactc aaaggaaaac ctgcatacgg
     2461 ccagctccag cgaccatcgc ttacggtcaa ttgttgacga gtaatttgcc acggcaaatc
     2521 gagcaacgga tcgccgttat cccgtgccag cacgatcaat tgcccgctat tttcctgcca
     2581 gtccagctcg gcatccacca gtgacggttc ctgcggcaag tttaacgtcg cagtagcatg
     2641 accacttaca ggaagtccat ccggcacgag cggcatagta aattccccca ccagtttaac
     2701 cggcggctga ttttcaaacg cgacgacatc cagttcgctg actgtaagtt gttgcccttt
     2761 cagctggcct tgaaatttaa ctttttcgcc ctgataacgc agttgctgga tatcagaggt
     2821 taatgcgaga gagagttttc cctgccattc ctgccaggga gaaaaaatca gtttatcgat
     2881 attgatccag gtgttaggca gcatggactg ccactgcgcg agggtttttg gagcggctgg
     2941 cgattgctcc gtctgcggca atttcgccag acaagcagaa tcaagttcta ccgtgccgac
     3001 gttcaataac cagcggctgg gatgtgaaag gctggcgttg gtgatatgcg caagctgaca
     3061 atctcccacc agataacgga gatcgggaat gattaaacct ttacgcgtaa tgcgtgggct
     3121 ttcatctaat gcaatacgtg tcccgagcgg tagccagatg cccgccagcg tgggaaccca
     3181 cagcccgagc gtcatcagca gcgtcaacgg cacaagaata atcagtaata acagcgcgag
     3241 aacggcttta tatttaccca gcatgggtag ttaatatcct gatttagcga aaaattaagc
     3301 attcaatacg ggtattgtgg catgtttaac cgttcagttg aaggttgcgc ctacactaag
     3361 catagttgtt gatgaatttt tcaatatcgc catagctttc aattaaattt gaaattttgt
//
LOCUS       DAZUCE010000346         3362 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZUCE010000346 DAZUCE010000000
VERSION     DAZUCE010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.348"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..130
                     /gene="ymgI"
                     /locus_tag="ACHY03_004931"
     CDS             <1..130
                     /gene="ymgI"
                     /locus_tag="ACHY03_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFS3908039.1"
                     /translation="IIPIITVTLILNYLNNSERSLVKQILIEDEIIVCATYLIPDI"
     gene            132..476
                     /gene="ymgG"
                     /locus_tag="ACHY03_004932"
     CDS             132..476
                     /gene="ymgG"
                     /locus_tag="ACHY03_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFS3908040.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            480..815
                     /gene="ymgD"
                     /locus_tag="ACHY03_004933"
     CDS             480..815
                     /gene="ymgD"
                     /locus_tag="ACHY03_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFS3908041.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(871..>3362)
                     /locus_tag="ACHY03_004934"
     CDS             complement(871..>3362)
                     /locus_tag="ACHY03_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ECIAI39_1904 family autotransporter adhesin"
                     /protein_id="HFS3908042.1"
                     /translation="TGNGSYGVSVAGEGSKLTINNGVIVTTGGLYPSDNSSLSTALTA
                     SAIVSEFGGAVILQGKNTIVTTGDYSIGLLSQTNGNLNTDTIIRVNPDGSATPSFSDG
                     GNSFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENSAKAILDMAKGEIT
                     THGTESYAAYANGTVVKADGTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFNQG
                     EIYTTGPDAAIAKIYNGGTVTLKNTFAVAHQGAGVVLESSINGQEATVDILSGSSLRS
                     ANEILYNKNETSNVTITDSEVSSAADVFINNIKGHLAVYATDSKITGSANLSTDDNTH
                     TYLSLSDNSTWDIKADSTVSKLTVDNSTVYIYRADGKDFEPTRLTITENYVGNNGVLH
                     LRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEFIK
                     DSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRPEA
                     GSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQL
                     RTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYS
                     AGLYATWFADDLSKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFA
                     LNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFI
                     RTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGARNLGEIKLGVNGNLN
                     PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT          861 a          816 c          761 g          924 t
ORIGIN      
        1 cataattcct atcatcaccg tcacgctcat tttgaattat ctgaataata gcgagcgttc
       61 tttagttaag cagatcttaa ttgaagacga aattattgtg tgtgcaactt acttaatacc
      121 ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca ttgttctgct
      181 ccacgccagc gatggcggat atgaaccgca ccacgaaagg tgcattgcta ggcgcaggcg
      241 ttgggttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct gttggcgctg
      301 gtgttggtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa ggtgcaaaag
      361 tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc gaaggggcga
      421 ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg aaataaggaa
      481 tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca atgacctggg
      541 cgcaggacta caatattaaa aacggtttgc cgtcagaaac ctatattact tgcgcagaag
      601 ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg gtaatgggta
      661 atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg agcgccaaag
      721 tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg atcacggcaa
      781 ttgatgacac catgcgtgct atcggcaaaa aataatattt catatgttac cgcgtcgatg
      841 aattcgacgc ggtattttag ctaacgggga tcagaactta tatttcaggc ccaccatcac
      901 tgcggtgtca ttgtagccat tatcacccag ctgcacgccc acattacccc agacactggc
      961 cgccggattc agattgccat ttacgccaag tttaatttcc cccaaattac gcgccccgtc
     1021 ttgttcgact ttcacaccgt tcattgagac agcaaaatct ttactgttat ggatccagtt
     1081 catttcaaca aaaggttcaa agtcgtcacc gtgcggaccg ctgtttttct cctgagtacg
     1141 aataaaggtg cggaaaccaa gtcgggtctg gatgttgtta tttgcgtcat tctcaatacg
     1201 tgagccattg gcttccgtat gcgcattatg atcgacgccc atccagatag cctgtgcctg
     1261 tggctggaag atccacgtat atttcgccgc ttccagacca aaggatttat tcagggcaaa
     1321 gccgtaaccc gcttccagcg agacggttgc gcctttcgcg ctataggatt cataggctaa
     1381 ttcatcccct ttcaccgagt ttttaaacca gctatattgc gcccaggcgt caatgtatgc
     1441 gcctttttta ctgaggtcat cggcaaacca ggtggcatac agccctgcgc tatagcctct
     1501 gacactccct ttcgaacgat aatccgacac gctggaatga gttgagttgt agtcgcgggc
     1561 ataaccagcc atcactccca aacgccaact gtcactgtcg gtaaaaccac cggttaacag
     1621 atcggcccct aactgcgaga cgtagcgatg cgaggtcgtt ctcaactgtc cgttgctgtc
     1681 acgccaggca ttatgcccgc caatttgacg tagccaaagt cggcttgaac gctcctgttc
     1741 agttacagga tcgatgtaac gcgtttcacc tgcacgatcg ttcagacgca taacaaaaag
     1801 agtgttagcg gctgccaggt tggcgacgta acttccagct tcggggcgca ggacgggggt
     1861 aggcgcaaca gtaggcgctg aacttcctcc ggagtttgtt tcaccgccgc tcgttgcctg
     1921 gaagttagtc agataccagt ttttattggt cgcttcggta ttacctcggg ttaatgaata
     1981 ttcgtaggca ccggcgaaaa tcctcgaatc cttaataaat tctccatttg attccccctc
     2041 aacgctgata atctctatcc catttaacgt ataagccccg ctgccgccag cattggtaac
     2101 tttaactcgg gttgttccag aggtatttcc attaataacg actttatccg tagctgaatt
     2161 atcatcaccc aattcagttc tgagatgcaa tacgccatta ttaccaacgt aattttcagt
     2221 tattgttaat cttgttggtt cgaaatcctt tccatctgcc cggtaaatat agaccgtact
     2281 attgtcaacc gtgagcttgc tcaccgttga gtcagcttta atatcccagg tactattatc
     2341 tgaaagcgac agataggtat gagtgttatc atctgttgaa agattggctg aacccgttat
     2401 ttttgaatca gttgcataga cggccaaatg acctttaata ttattgataa aaacatctgc
     2461 agccgatgat acctcactat cagtaatggt cacgttactc gtctcatttt tattgtagag
     2521 gatttcattt gctgaccgta gtgagctacc agataaaata tctaccgttg cttcctgacc
     2581 atttatggaa gactccagca caactccagc cccctgatgc gcgactgcaa atgtattttt
     2641 cagcgtcacc gtgccgccat tataaatttt agcaatcgcg gcgtcaggac ccgttgtata
     2701 aatttctccc tggttaaatg aaactgtccc ctggcgagca gcgatggcat gagcattgtc
     2761 cccatgcgtg gtgatatcaa catcggttaa tgtaacgctg gcattggtat aatcaagcgt
     2821 gccatcagcc ttgacgacag tgccattagc gtaagcggca taactttctg ttccgtgggt
     2881 tgttatctcc ccttttgcca tatccagtat tgctttggcg ctattttcat tacttcctgt
     2941 atcggcagta ctttcttcat caagagaaga tacacacgca cgcgcgcttc cgggagatgc
     3001 acacgcaaga acgccgactg cgtgattacc cgcagtaacg ataaaagaat tacctccgtc
     3061 agaaaaagag ggggtagccg aaccgtcagg gttgactctt attatggtat cagtattcag
     3121 gtttccattc gtttgactta gcaaaccaat tgagtaatcc cccgttgtga caatcgtgtt
     3181 tttaccctgc aaaataacag caccgccaaa ttcacttacg attgcgctag ctgttaatgc
     3241 tgtgcttaga gaactattgt cagaaggata taaaccgccc gttgttacaa tgacgccatt
     3301 gttaatggtt aatttgctac cctctcctgc aacagatacc ccgtaacttc cattacccgt
     3361 tg
//
LOCUS       DAZUCE010000347         3324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZUCE010000347 DAZUCE010000000
VERSION     DAZUCE010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.349"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..245
                     /locus_tag="ACHY03_004935"
     CDS             <1..245
                     /locus_tag="ACHY03_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFS3908043.1"
                     /translation="RAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQA
                     NAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
     gene            246..1304
                     /gene="rsxD"
                     /locus_tag="ACHY03_004936"
     CDS             246..1304
                     /gene="rsxD"
                     /locus_tag="ACHY03_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFS3908044.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCAMLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1308..1928
                     /gene="rsxG"
                     /locus_tag="ACHY03_004937"
     CDS             1308..1928
                     /gene="rsxG"
                     /locus_tag="ACHY03_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFS3908045.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1932..2627
                     /gene="rsxE"
                     /locus_tag="ACHY03_004938"
     CDS             1932..2627
                     /gene="rsxE"
                     /locus_tag="ACHY03_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFS3908046.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            2627..3262
                     /gene="nth"
                     /locus_tag="ACHY03_004939"
     CDS             2627..3262
                     /gene="nth"
                     /locus_tag="ACHY03_004939"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFS3908047.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT          784 a          851 c          884 g          805 t
ORIGIN      
        1 cccgtgccaa agcgcgcaaa ctggaacagc aacaggctaa tgcggagcca gaagaacagg
       61 ttgatccgcg caaagctgcc gtcgaagcgg ctattgcacg cgccaaagca cgcaagctgg
      121 aacagcaaca ggctaatgca gaaccagaag aacaggttga tccgcgcaaa gcggcagttg
      181 ccgcggctat tgcccgcgtt caggccaaaa aagccgccca gcaaaaggtt gtaaacgagg
      241 actaaatggt attcagaata gctagctccc cttataccca taaccagcgc cagacatcgc
      301 gcattatgct gttggtgttg ctcgcagccg tgccaggaat cgcagcgcaa ctgtggtttt
      361 ttggttgggg tactctcgtt cagatcctgt tggcgtcggt cagtgctctg ttagccgaag
      421 ctctcgtact caaactacgc aaacagtcgg tagccgccac gttgaaagat aactcagcat
      481 tgctgacagg cttattgctg gcggtaagta ttccccccct cgcgccatgg tggatggtcg
      541 tgctgggtac ggtgtttgcg gtgatcatcg ctaaacagtt gtatggcggt ctgggacaaa
      601 acccgtttaa tccggcaatg attggttatg tggtcttact gatctccttc cccgtgcaga
      661 tgaccagctg gttaccgcca catgaaattg cggtcaacat ccctggtttt atcgacgcca
      721 tccaggttat ttttagcggt cataccgcca gtggtggtga tatgaacaca ctacgcttag
      781 gtattgatgg cattagtcag gcgacaccgc tggatacatt taaaacctct gtccgtgccg
      841 gtcattcggt tgaacagatt atgcaatatc cgatctacag cggtattctg gcgggcgctg
      901 gttggcaatg ggtaaatctc gcctggctgg ctggcggcgt atggttgcta tggcagaaag
      961 cgattcgctg gcatattccc ctcagcttct tagtaacgct ggcgttatgc gcaatgttgg
     1021 gctggttgtt ctcaccagaa acactggcag caccgcaaat tcatctgctg tctggagcga
     1081 ccatgctcgg cgcattcttt attttgactg acccggttac cgcttctacg accaatcgtg
     1141 gtcgtcttat tttcggcgcg cttgcgggct tattagtctg gttgatccgc agtttcggcg
     1201 gctatcctga cggcgtggct tttgccgtcc tgctggcgaa catcacggtt cctctgatcg
     1261 attactacac gcgtccgcgc gtctacggcc atcgcaaagg gtaaaccatg ctgaaaacta
     1321 tccgaaaaca cggcattacg ttagcgctat ttgcagcggg ttcaacaggg ttaactgcgg
     1381 ccatcaacca gatgaccaaa acgacgattg ctgaacaggc cagtctgcaa caaaaggcgt
     1441 tatttgatca ggtgctgcca gccgaacgct ataacaatgc gctggcacag agttgctatc
     1501 tggtaactgc gccagagtta ggtaaaggtg agcatcgggt ttacatcgcc aaacaggatg
     1561 acaaaccggt agccgccgtt ctggaagcaa ccgcgccaga tggctattcc ggtgcgattc
     1621 agctgctggt gggagccgat tttaacggca cggtacttgg cacgcgcgtg acagagcacc
     1681 acgaaacgcc agggcttggc gataaaatcg aactgcgcct ttctgactgg atcacccatt
     1741 ttgcgggtaa aaaaatcagt ggtgcggatg atgcgcactg ggcggtgaag aaagatggtg
     1801 gtgatttcga ccagttcacc ggcgcgacga ttactccccg cgcggtggtt aatgcggtaa
     1861 aacgcgccgg attgtacgct cagacgttac cggcacaact ttctcaactt cctgcctgtg
     1921 gagaataaag cgtgagcgaa attaaagacg ttattgttca ggggttgtgg aaaaacaact
     1981 ctgcgctggt ccagttgctc ggcctttgtc ctctgttggc ggtcacgtcc actgccacta
     2041 acgctctggg tttaggactt gcgactacgc tggtactgac gctgaccaac ctgaccattt
     2101 cgacgctgcg tcactggacg ccagccgaga tccgcattcc catttacgtg atgatcatcg
     2161 cctcggtggt cagcgctgta cagatgctga tcaacgccta cgcctttggc ctgtatcaat
     2221 cattagggat ttttattccg ctgattgtca ctaactgtat cgttgtgggc cgcgctgaag
     2281 ccttcgccgc caaaaaaggt ccggcgcttt cggcactgga cggcttttca attggtatgg
     2341 gcgcaacctg cgccatgttc gtgctgggtt cactacgcga aattatcggc aatggcacat
     2401 tgtttgacgg tgcagatgcg ctgttaggta gctgggcaaa agtattacgc gtggagattt
     2461 tccacaccga ctcccctttc ctgctggcga tgctgccacc aggtgcattt attggcctgg
     2521 gactgatgct ggcaggaaaa tacctgattg atgaaagaat gaaaaagcgc cgtactgaag
     2581 cagctgccga acgtgcattg ccaaacggtg aaacagggaa tgtctgatga acaaagcaaa
     2641 acgcctggag atcctcactc gcctgcgtga gaacaatcct catcccacca ccgagcttaa
     2701 tttcagttcg ccttttgaat tgctgattgc cgtactgctt tccgctcagg cgaccgatgt
     2761 cagtgttaat aaggcgacgg cgaaactcta cccggtggcg aatacgcctg cagcgatgct
     2821 tgaactgggc gttgaagggg tgaaaaccta tatcaaaacg attgggcttt ataacagcaa
     2881 agcagaaaat atcatcaaaa cctgccgtat cttactggag cagcataatg gcgaggttcc
     2941 ggaagatcgt gctgcgcttg aagccctgcc cggcgtaggt cgtaaaacag ccaacgtcgt
     3001 attaaacact gcattcggct ggccgactat tgctgtcgac acgcacattt tccgcgtttg
     3061 taatcgtact caatttgcgc cggggaaaaa cgtcgaacag gtagaagaaa agctactgaa
     3121 agtggttccg gcagagttta aagtcgactg ccaccattgg ttgatcctgc acgggcgtta
     3181 tacctgcatt gcccgcaagc cccgctgtgg ctcttgtatt attgaagatc tttgtgaata
     3241 caaagagaaa gttgacatct gaagaaaagg ggtaacaccg attaccccat tgataacctt
     3301 tctttatcct cttttaaaac atct
//
LOCUS       DAZUCE010000348         3307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZUCE010000348 DAZUCE010000000
VERSION     DAZUCE010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.350"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            512..720
                     /locus_tag="ACHY03_004940"
                     /pseudo
     CDS             512..720
                     /locus_tag="ACHY03_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            707..896
                     /gene="tnpB"
                     /locus_tag="ACHY03_004941"
                     /pseudo
     CDS             707..896
                     /gene="tnpB"
                     /locus_tag="ACHY03_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(899..1087)
                     /locus_tag="ACHY03_004942"
     CDS             complement(899..1087)
                     /locus_tag="ACHY03_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFS3908048.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            1622..1763
                     /locus_tag="ACHY03_004943"
                     /pseudo
     CDS             1622..1763
                     /locus_tag="ACHY03_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            1986..2723
                     /locus_tag="ACHY03_004944"
     CDS             1986..2723
                     /locus_tag="ACHY03_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFS3908049.1"
                     /translation="MIVCYVILVNRPFETEIAGKKIFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRAWRVKDISNSL
                     YMSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAIRCGYASTPYFISV
                     FREYFGITPHQMMINQSGFIESRIAIK"
BASE COUNT          911 a          745 c          674 g          977 t
ORIGIN      
        1 tgttatttct tgacctttat cacagaacca tctccctgga tatccccatc acattgcatg
       61 aaaagcaagt tttaccaatc ttgattttca gtgttaaata agattaataa ctgtaattct
      121 gcaaaaaaaa gaacaccata tgtagtatga ctacgtattc cactctgttc gttcacggac
      181 tggcatcgaa gtctgaggtg tactggcaat agcggataca gtcatttgtt ctttttttaa
      241 gcattcatcc gatgatactt ttcctgaatg ctaccggagt ggtattcccc tgccgtgagg
      301 gagggcgctg acggttataa aaatcctgat atatacccat tcccgtatga cagcgatggc
      361 gtcacatctc tgttctacca tcttcacaga aatatttcgt tcattagcat tccggtattc
      421 gtataaccac cttcggtgga gaaagaataa acaatccctg gaatgcgatg agcgcttgac
      481 cagaaaagca tcatattgct tcatggcgca tcagcgacca caggatgccg caaaagccga
      541 aaaacggacc agagcgcctg caatcattcc tcgcctcagt atctgttgcc catggagtgg
      601 ccgataacag atcattgtcg gatgagcctt atccccttcc ttcccggtgg tatccggatg
      661 tgctgccagc cttctcagtt aaccgatgtt ttccgggctt tttgccatac tgaggactga
      721 taacgtattc ctggtcgtaa aacccgtcaa tatgcgccgg ggcatcgaca aaactgacac
      781 agtacgtccg gaaagaactt aacgctgcat ggcacgacaa tgctgccttt gtctttacca
      841 acaaggctcg ttcccgcata aaagttctac gccgggataa acatggcgta tggctgcgtc
      901 aggccttcac tacttcacac cggaagtctt ccaccctgac acgaatatac cgtccgggct
      961 ctttcgcagg tgcaaacagt ttttcacatt cggcgaatgt catttctgaa tcctggtaac
     1021 gcaaccactg aaccggcata ttaccgggtt tgattattgt cgcagttatt ttatatcgtt
     1081 tccccatatc gcctctcatc ctgacaacaa aattaccggc tgagccatca ctccggtaaa
     1141 gcatgcgaaa aaaagtgagc gtctcagcaa tggctcatgc gatgattctg tacgggcacc
     1201 gatggcacct cagaatacaa caaagatatc agagtaacat gtctctctcg ttatttttca
     1261 gagttcttga caccctcctt ctgccacatt gtcaacgcca gtggagactt ctgctgtcag
     1321 ttttttcatc catacagagt gacattaatt atcctgaaca aaacgaaata tcctgaatat
     1381 atttattttc tcctggtgcc ggagacatga tgatgtacag gctcaggtgc ggtgtgaatg
     1441 taaaatggta tgttaattat tcatttctga atgcaaactg aatgcaaact tattccgaat
     1501 tgaattctcc tgacaccaca tgataaaaat gaggatataa taaaatactt ctcttctgga
     1561 tgtgccatta cctgccgttg ctgtaatacg gcgggtattt tttactgaca tatcgggaag
     1621 ccagcagacg ttcgtagcta catccccgaa agtggcacac tgcaaactat aatacgagta
     1681 aaacatcgat caccgtagct gatgtgttca acgatcgtgc actagcgttc tgcgattctc
     1741 agggactgcc tgttctgaga atcggcgagt ccgcgatcgg atacgatgcg atttcgcagt
     1801 gactcaggga ttgcatctga aaaccctccg gctatgtcgg taaatattta tttctatgaa
     1861 ccgtacgatt cagatagcat tcgcccggcc ccgtaagaat aatctccgga caaataagat
     1921 ttttcctgaa aaagcgtcta gtcttttgat atcgttgttt ttatgctaat cagggagtat
     1981 gtgtgatgat tgtctgttac gttattcttg ttaaccgtcc gtttgaaaca gagattgccg
     2041 ggaaaaagat attttttcag aaaaatgacg tcctgttgac agataacatc agccagtcct
     2101 ttttcctgcg ccatcccggt aatgttactg agctccggat tgatgaatgt attgtcagaa
     2161 aatatatgga aagtgccgac aggatccagc actctgatag tgctgtttct gttccgttca
     2221 ttaaaacaac cttctctcac atcgctctgt tacataatgt catgcagaat ctcagtctgc
     2281 aaaagacatt gccacaacaa ttttctgagc agatgttttt tgcctgcctt tccatttttt
     2341 ccacacacag aggtttcatg tttctgctat tcagatgcat aaactctgtt gcgcttaaag
     2401 tcaggtatat tatctgttcc agtccatcac gggcatggag agttaaagat atcagtaatt
     2461 ctctgtacat gagtgagagt ttactgaaaa gaaaacttca ggaggaaaaa gtttctttct
     2521 cccaaatcct gctggatata cgcatgcata acgcccgaca acttatttcc agtaaactgt
     2581 cagtgaatca aatcgcaata cggtgcggtt atgccagcac accgtatttt atcagcgttt
     2641 tcagagaata ttttggcata acacctcatc agatgatgat taatcagtcc ggatttattg
     2701 agagcagaat cgcaattaag taaagtatgt cagtcagcgc ataatgaaag tattcttatg
     2761 cttcattatc agtttctgtt tatcactccg gagcactgta gtaactacgg ccccggaaaa
     2821 cagatggtaa tgactgactg atgatattgt ggcgacattt ttacggatgc gagcaaagaa
     2881 tgtattggtc cgtttgatta tgcagtgatt aacaaaactc cgggaagatg aatgagtata
     2941 cctctcctgc aatccgtttc ctgagaaaac aggtggtaac acaatattct taatatgtta
     3001 ctgtttcacc acacaaccag gtttacggtt gtgtggtgaa ctatagtaat cccgggtaac
     3061 aatccggagc tgttcctttt atactcttca gactgtgtac gcatccacgc catccgtttt
     3121 acagtctcag tgtgttaata ataaattaca ctttgaagtg aactccagtc aggaccatgt
     3181 cgtatttccg ggtggatgag catatatcag aaactacaat tactacagat aacggttttc
     3241 agtaattctg tcataacttg ccggaatgta atgttttgtg acatggtcct tattttttat
     3301 acacagg
//
LOCUS       DAZUCE010000349         3279 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZUCE010000349 DAZUCE010000000
VERSION     DAZUCE010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.351"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            172..3081
                     /locus_tag="ACHY03_004945"
     CDS             172..3081
                     /locus_tag="ACHY03_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFS3908050.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYVDNAATGLN
                     WTNMDNPGTVDGNYVNYSGFVYYNNTNGDFDQSFNGDTVNGTISTYYLNHDYTDGTNG
                     NANQLDIANSVIHGSITSMLPGGDLWHNGAYDQSYGYIQAGDTVIDTTWYDGDVFTLN
                     IANSTIDDDYEALYFTDYYKDGDVTKSTNGTFDVSEGVAVNLNVESNINISNNSRVAG
                     IALSQGNTYNNTYTTESHTWDNNINVSNSTVTTGSNTVLESSGYGHFGNSGEPSDYAG
                     PGDVALSFTDSTSDYAMKNNVYFSNSTLMGDVAFTSTWNANFDPSGHDSNGDGVKDTN
                     AGWVDDSLNVDELNITLNNGSKWVGQATFNAETISPDTMYDVATNSLTPGGTAEANGW
                     NRIIDNKVFQSGVFNVALNNGSEWDTTGDSLVDTLTVNNGSQVNVSDSSLTSDTIDLT
                     NGSSLNIGESGYVDTDHLTINSYSSVDLTESTGWGIYSNLYANTITVTNGGVLDVNVD
                     QFDTEAFRTDKLELTSGNIADNNGNVVSGVFDIHSSDYVLNADLVNDRTWDTSKSNYG
                     YGIVAMNSDGHLTINGNGDMNNGDELDNSSVDNVVAATGNYKVRIDNATGAGAIADYK
                     DKEIIYVNDVNTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSA
                     NTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGV
                     DTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSY
                     SHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSISGLFQSGD
                     DYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVN
                     GDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            3169..>3279
                     /locus_tag="ACHY03_004946"
     CDS             3169..>3279
                     /locus_tag="ACHY03_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3908051.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSS"
BASE COUNT          900 a          712 c          740 g          927 t
ORIGIN      
        1 tctgtgtgtg tgttcacgtg ctttatatat gtccgcaatc ggacttaggg gaaagaataa
       61 ttgaaccatt cctctataac gccgcataat cgtcaacgcg aaacataata tgttttctat
      121 gcattgataa ttgatggatc aacttattac gtccctgagg agggatgaca aatgcactcc
      181 tggaaaaaga aacttgtagt atcacaatta gcattggctt gcactctggc tatcacctct
      241 caggctaatg caactactta tgacacttgg acttatgtcg ataacgcggc tactggtctt
      301 aactggacca atatggataa tccgggtact gtagatggta actatgtgaa ttatagtggc
      361 tttgtctact ataacaacac caatggcgac ttcgatcagt cttttaacgg tgatactgtt
      421 aacggcacga tttctacata ctatctgaac cacgactaca ctgacggtac aaatggtaat
      481 gctaaccagc ttgatattgc taattcagta attcatggct caatcacttc catgcttcct
      541 gggggtgatc tctggcataa tggtgcttat gatcaaagct atggctacat tcaggctggt
      601 gatacagtta ttgatacaac gtggtatgac ggtgatgtat tcactctgaa tattgctaac
      661 tccaccattg atgatgacta tgaagcactt tacttcactg attactacaa agatggcgat
      721 gtaactaaat caacgaatgg cacctttgac gttagcgaag gcgttgctgt taatcttaat
      781 gtagaaagta acatcaatat ctccaataac tcccgcgttg caggtattgc gttatctcaa
      841 ggtaatactt acaacaatac ctacaccact gaatcccata cttgggataa caatattaat
      901 gtctctaact caaccgtaac taccggttca aataccgtgt tggaaagttc tggttatggt
      961 catttcggta actctggtga gccgagtgat tatgctggcc cgggtgacgt tgcattgtct
     1021 ttcactgata gtacttcaga ctatgcaatg aagaacaatg tatatttcag caattctact
     1081 ctgatgggtg atgttgcgtt taccagcacc tggaatgcta attttgatcc aagtggtcat
     1141 gattctaacg gtgacggcgt aaaagatacc aatgctggtt gggttgatga tagcctgaac
     1201 gttgatgagc tgaatattac tcttaataat ggtagcaagt gggttggtca ggctacattc
     1261 aatgctgaaa ccatttctcc agacacaatg tatgatgttg caactaacag tttgacccct
     1321 ggtggcacag ctgaagctaa tggttggaat cgcatcatcg acaataaagt attccagagt
     1381 ggtgtattta acgtagcgtt gaataacggt tctgaatggg atactacagg tgattcgctt
     1441 gttgataccc tgaccgttaa taatggttct caagtcaatg tttctgattc tagtttaact
     1501 tcagacacta tcgatttaac taatggctct tctctgaaca ttggtgaaag tggttatgtt
     1561 gataccgatc atctgactat taactcctat agttctgttg atttgaccga atcaacaggc
     1621 tggggcatct attccaacct gtatgctaat actatcaccg taactaacgg tggtgtcctg
     1681 gatgtgaacg ttgatcagtt cgataccgaa gctttccgta ccgataaact ggaactgact
     1741 agcggcaaca ttgctgacaa taacggtaat gtagtatctg gtgtattcga tatccatagc
     1801 agcgattatg ttctgaacgc tgatttggtg aacgaccgta cctgggatac ttccaagtct
     1861 aactacggct acggtattgt tgctatgaac tctgatggtc atctgactat caacggtaac
     1921 ggtgacatga acaacggcga cgaactggat aacagctctg ttgacaacgt tgttgctgca
     1981 accggtaact acaaagttcg tatcgacaac gcaacaggcg ctggcgctat cgctgattac
     2041 aaagataaag aaattatcta cgtaaacgac gtcaacacca acgcgacctt ctctgctgct
     2101 aacaaagctg acctgggtgc atacacctat caggctgaac agcgcggtaa caccgttgtt
     2161 ctgcaacaga tggagctgac cgactacgct aacatggcgc tgagcatccc atctgcgaac
     2221 accaatatct ggaacctgga acaagacacc gttggtactc gtttgaccaa ctctcgtcat
     2281 ggcctggctg ataacggcgg cgcatgggta agctacttcg gtggtaactt caacggcgac
     2341 aacggcacca tcaactatga tcaggatgtt aacggcatca tggtcggtgt tgataccaaa
     2401 attgacggta acaacgctaa gtggatcgtc ggtgcggctg caggcttcgc taaaggtgac
     2461 atgaatgacc gttctggtca ggtggatcaa gacagccaga ctgcctacat ctactcttct
     2521 gctcacttcg cgaacaacgt ctttgttgat ggtagcttga gctactctca cttcaacaac
     2581 gacctgtctg caaccatgag caacggtact tacgttgacg gtagcaccaa ctccgacgct
     2641 tggggcttcg gtttgaaagc cggttacgac ttcaaactgg gtgatgctgg ttacgtgact
     2701 ccttacggca gcatttctgg tctgttccag tctggtgatg actaccagct gagcaacgac
     2761 atgaaagttg acggtcagtc ttacgacagc atgcgttatg aactgggtgt agatgcaggt
     2821 tataccttca cttacagcga agaccaggct ctgactccgt acttcaaact ggcttacgtc
     2881 tacgacgact ctaacaacga taacgatgtg aacggtgatt ccatcgataa cggtactgaa
     2941 gggtctgcgg tacgtgttgg tctgggtact cagttcagct tcaccaagaa cttcagcgcc
     3001 tataccgatg ctaactacct cggtggtggt gacgtagatc aagattggtc cgcgaacgtg
     3061 ggtgttaaat atacctggta atattcttca ctccgaagaa attctggtaa tttaatctaa
     3121 ataatgcccg tcaaggattt gacgggcatt actgcaaagg acgcgcaaat gttatctgta
     3181 gttaaacctc ttcaggaatt tggtaagctc gataaatgtt tgtccagata cggtacgcgc
     3241 ttcgagttta ataatgaaaa gcaagttata ttttccagt
//
LOCUS       DAZUCE010000350         3234 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZUCE010000350 DAZUCE010000000
VERSION     DAZUCE010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.352"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..300
                     /gene="tssJ"
                     /locus_tag="ACHY03_004947"
     CDS             <1..300
                     /gene="tssJ"
                     /locus_tag="ACHY03_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFS3908052.1"
                     /translation="NRNFDSADYQALFTGDSEILAGDIIAQKDVWMQPGGSVTVDMPL
                     DDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTPRLIEVSGNTLTLLPVKDK"
     gene            303..734
                     /gene="tssE"
                     /locus_tag="ACHY03_004948"
     CDS             303..734
                     /gene="tssE"
                     /locus_tag="ACHY03_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFS3908053.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            992..1996
                     /locus_tag="ACHY03_004949"
     CDS             992..1996
                     /locus_tag="ACHY03_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS3908054.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            complement(2248..2679)
                     /locus_tag="ACHY03_004950"
     CDS             complement(2248..2679)
                     /locus_tag="ACHY03_004950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908055.1"
                     /translation="MLLEAIDGCQDLEDEYPQGFIEEVMSSLTFIRHDNFQCLERNNI
                     FVASFSNVPDLLGQWRNYGKYCIELDSEFFKNKEVDVLECHYLYDDALEYANTLVETH
                     ILLGLLEIWHKNKAFLTIELSSLIDIYALSFNLWSPPFLQH"
BASE COUNT          911 a          695 c          754 g          874 t
ORIGIN      
        1 aaccggaatt ttgacagcgc ggattatcag gcgcttttta cgggggacag tgaaattctg
       61 gcaggcgata tcattgcaca gaaagatgta tggatgcagc caggtggctc agtaaccgtg
      121 gatatgcctc tggatgatgc ggcgaaattt accggtgtgg cagcaatgtt tctggagcct
      181 gaccggaaga aaaatacctg gcgggtggtg ctgagccggg atgagcttga gccggacacg
      241 ccccgtctta ttgaagtatc cggtaatacc ctgacgctgt taccggtgaa ggataaatag
      301 tgatgccgcg tccttccctt tatgaaattc tctatggcaa tttcgctggc gggctggagt
      361 taaaccaggt cagtgcggaa gagcaggtta ttctttcggt gctcgataac atgcagcgta
      421 ttctgaatac ccgggccgga agccttaaac atctgccgga ttatggtctg ccggatatga
      481 caaccgttct gcagggaatg ccgggaacgg cgcatcaact gatgcgtgtt ctttcagatg
      541 ttctgctgaa gtatgagcca cgtattaagc gtgtcaacgt gacgatgcag gagcagactc
      601 agcctggtga gttgcattat gtcattgatg cagaacttaa agatgccggg ctggttcgtt
      661 acggaacaac atttataccg gagggcaggg tgttgttgcg ccatctgaaa cagcagcggt
      721 acgttcagac gtgagccgga gcagggaggg cagaacagaa ataaacagtg atggatatat
      781 gtcaggtgtg aatgtgacat tattccggct tatgacaacg ctggcggaat gtttcatcat
      841 cctgataact ctggcatatc agcaataaca atgtatgagt ttttaaatga agaaaattat
      901 ctgttttctg ttcgtggggc taatgagatg gtcaccccca ccttaacagc agtacgctgt
      961 taaggtgaat ccctttaacg gagagaccat catgcagaac atcacactta ttggtatcga
     1021 tctcggaaag cactccttcc acattcattg tcaagacaag tctggtaagg ctcttctgcg
     1081 taaaaaattt acccgtacta aactgatgca gtttctggcg acatgtccgc cctcagttgt
     1141 tgtaatggaa gcctgtgcgg gtgcacactt tatggctcgt cgtatctctg attttgggca
     1201 tgaaactaag ctaatttctc cacagttcgt tcgcccgttt gtcaaaagta ataagaatga
     1261 ttttgttgat gcagaagcca tatgcgaagc ggcttcacga ccatcaatgc gttttgtcca
     1321 gccaagaact gagcccctgc aggccatgag ggcgttacac cgggtccgag aatcactcat
     1381 cagagataag gtgaaaacaa ccaaccagat ccacggtttc ttgctggaat ttggaatcag
     1441 cctgccgaca ggtgatgctg taataaaacg gttgtctctc gtgttggccg aacacgaaat
     1501 ccccgaatat ctgagccgcc ttttggtgag gttgcatacc cactaccttt accttgttga
     1561 gcaaatagca gagctggaat cagaattaag tcagtcaatc aacgctgatg ataccgctca
     1621 gcgcattatg acaattcctg gtgttgggcc gattactgcc agcttactat cgtcgcaacc
     1681 tggtgacggg aaacagtttt catgcagcag agattttgcg gcttccacag ggctcgttcc
     1741 ccggcagtat agcacgggag gaaaatcgac tctgttgggt atcagtaagc gaggcgataa
     1801 aaatttgcgc cggttactgg ttcagtgtgc ccgctcattc atgatgcggc ttgaacgcca
     1861 gcatggaaaa cttgccgaat gggttcggga gcaactcaac aagaagcatt cgaatgtggt
     1921 tgcctgcgct ctggcaaata aactggctcg aattgcctgg gtaatcacta ctcagcaaaa
     1981 agaatatcag gcttgatata aatgctgata taactcaatt ggcttattgt aaacaataag
     2041 atacaaagtt gcctgtctgg ttttgcgaag actgattatt gatgacatga acggcaaatc
     2101 gacctgatta aaaacctaac agagaaaatg gctcactgaa gccgagggcc tttttaggat
     2161 tatcaggtgc ggaactcatc gaggcgcggg cctccttgcc cacagagacg ccggatagat
     2221 ttaagcaagc ccaccattaa tcaaaaatca gtgttgcaaa aacgggggtg accatagatt
     2281 aaatgaaagt gcatagatat caatgagtga actcaactca attgttagga atgctttgtt
     2341 tttatgccag atttccagca aacctagtaa tatgtgagtt tcgaccagcg tatttgcata
     2401 ttccagagca tcatcataaa gatagtggca ttctaaaaca tcaacctctt tgtttttaaa
     2461 aaattcagaa tccagctcaa tacaatactt cccgtagttt cgccactggc cgagaaggtc
     2521 gggtacatta ctgaatgagg caacaaaaat attattacgt tctaagcact gaaaattatc
     2581 atggcgaata aacgtaagcg agctcataac ctcctctata aacccttgag gatattcatc
     2641 ttctaagtcc tgacacccat caattgcctc taaaagcact tcatatcccg ttaaaatgtc
     2701 ccgttaaaaa ctcttcttta tcatttagaa aatgataatc ggtcagccag acctcagcac
     2761 tctttgctat ggatattgcc gcattcaaat cagtatagtg ataaatcatt gcgagtcagt
     2821 catctcttcg gattatacac tgatgataac ataaggcccg tgacagtact tactacatac
     2881 ttgtgcatat agcttatttt attcttattt tattctgttt ttattattta gttggtttgt
     2941 atgtctgttt tttctggtaa taaattgaaa atatctgacc agttccacgt tgattagtac
     3001 gcgataaggt taataacttc accatcgact ttgagagcaa ctgcaaactt ccgctcctcg
     3061 ctcatagcgg acctttcgct cagttaacgc atcagtgtcg tgccagaagt agacatggtt
     3121 aacaaaaatg tgagttaatc aacggagagc aggtcaatct aacctgtatc aaaaaaacaa
     3181 atgtgtcaca aagatgccac atgccttaat ttgagtataa ttgagacgga agta
//
LOCUS       DAZUCE010000351         3165 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZUCE010000351 DAZUCE010000000
VERSION     DAZUCE010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.353"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1492)
                     /locus_tag="ACHY03_004951"
     CDS             complement(<1..1492)
                     /locus_tag="ACHY03_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFS3908056.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGS
                     GNGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPEAGGNSDV
                     QGGYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVG
                     SEKAKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVD
                     VEQFHYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNE
                     EPQRQNQAKKAAYDVFYSFSMNRDRIQSDVLNKAAELISDMGNKIGDYLGESYKSLAR
                     EIADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADRDALVNAWRSRDAQDIANK
                     LGNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVESWVLSGMAASVAMTLLS
                     AIIGSFTLPALALTAINIAGIMGISYLASYIDASVAEKINNKLVHFT"
     gene            1731..2021
                     /locus_tag="ACHY03_004952"
     CDS             1731..2021
                     /locus_tag="ACHY03_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908057.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            complement(2013..2291)
                     /locus_tag="ACHY03_004953"
     CDS             complement(2013..2291)
                     /locus_tag="ACHY03_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908058.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRASLAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            2412..2834
                     /locus_tag="ACHY03_004954"
     CDS             2412..2834
                     /locus_tag="ACHY03_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFS3908059.1"
                     /translation="MRSAVARLLLIPLITATIVLTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGVKDKAAGVSAETAQQRIKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
BASE COUNT          938 a          797 c          555 g          875 t
ORIGIN      
        1 gagtaaaatg aaccaattta ttatttattt tttcagccac acttgcatca atatatgatg
       61 caaggtaact aattcccata atacctgcaa tatttattgc agttaatgct aacgccggta
      121 gtgtgaatga tccaattata gcactaagca atgtcatggc aacgcttgcg gccataccac
      181 tgagaaccca agactcaacc tccaacatca gcggccccca gtttccagtt tcatatccct
      241 cgatgctttt ctccttgacc ttctcaatct tcataattac atcggcagct ttgaaagcct
      301 tactgatatt ccccagttta tttgctatat cctgagcatc acgacttcgc caggcgttca
      361 ccagtgcatc acggtctgct ctgttaactt taaaactagg attagacaaa attttattta
      421 atgactccat tgcatcatcg tagctacgga tggttttccc ctggaaattt ttgatatcat
      481 cagcaatttc tcttgctaac gacttataag attcaccaag ataatcaccg atcttgttac
      541 ccatatcaga tataagttca gctgctttat ttagcacatc actctgaatc cggtcacggt
      601 tcatactgaa agaataaaat acatcataag cagctttttt agcttgattc tgtcgttgag
      661 gctcttcatt attaaatcct aatgcctcgt atatctcttc cggggtctta tggtcgactt
      721 tatccataat accagcctta gctttttcac tccctacata aggacgaaca gctgttctag
      781 ttacagataa aacattacca taatcatcaa gcaccactaa atagtggaac tgttcaacat
      841 caacatcaaa ttttctcaca ttaggaggca aacgatccca cgcatcttca gcctgttgtt
      901 ttgctttttc ctgccgctga gattcatctt tattaaaacc taacgcctca tatatctcct
      961 caggtgtttt atgattgatt ttttccataa tacccgcctt agctttttca ctccctacgt
     1021 aaggacggac acctgttcga agaataccga tagctctacc attatcatta agcgttacac
     1081 gatatttaaa accatcgata ctcacatcaa tctctcgcgg taagttgttt ttatcccact
     1141 tacgagcaac ttcatcatta ggattttcag gaatatagcc cccctgaaca tcactatttc
     1201 cgccagcctc aggaactcca tatacaggcg tttttaccag accatttgta atggtaatgc
     1261 gcacctgccc attatcatca acataaggcg gcacatccgg attattggtt ttactccatc
     1321 cccaccccgt ctgacttcca tgttcaccac ctccattacc agagcctgaa ccaccacccc
     1381 agtgaacccc actattacta ttattatttc caccttgctg attcagattc gcccctgttc
     1441 cccccataga ctcaccagca gttgggccat atccacttag ttctttagcc ataaattcct
     1501 ctttgataat taaaacaata aattaaaaac aacacactgt acatataacc actggtttta
     1561 tgtacagaaa aacctactac tcaccattgt ccatgtcaag agcatggatt ttcatttttg
     1621 caataaggat cacactatgg ggaggcaggc attgagaacg tcgaaacaga acaccggagc
     1681 aaatcaggat gagatataaa actgttggat catgaaaaac ggagaacgat gtgagcaaat
     1741 caccccgcca taaactgaac aaaacagaca aacgacttct cgacaccctt gttgctgccg
     1801 gatatgagca tgacaaagcc cgtgacctca tccagaaaca ggtttacacg ctgacactgg
     1861 ctgatcagcg ccatgtggtc agtgaaatca gtaacggtgt gaatcccacc caggcttact
     1921 cggcggtata ccaggcaaga cgcattcgcc tcgcccgtaa atatctgaac ggtaaaaagg
     1981 ttatggaaga aaccggggaa aatacgcccc catcagcgta aggatttctt ttgccgctcc
     2041 agagactcca gttttttacg caaatcctct cttttttggg catctctggt gccagccaga
     2101 gatgccctca actcatcgat ctgaagttgt atcttcagtc tgttactgaa cattttctgt
     2161 ctggcattaa catccgcaac aatgccattt tttgtcttct cggccttttg ttgaaaaaca
     2221 ttgctgtccg catgactggc aaccgaagca gaaagaacac taaaaagcag gactggcaca
     2281 cattttttca cgggattatt cctgactcat tgaccatcaa atcacattgg ggtaaaccga
     2341 cgtatgataa gagatactct tcggaaatat aactccctga gtatcaagat taaaaacgca
     2401 aggagatgtt tatgagatct gccgttgcca ggctgcttct gatacctctg ataacagcaa
     2461 caatcgttct tacaggatgc acaccaaaga ccagcctgga acgacatacc cggcattatg
     2521 tttatgcttc agatgatgga tttgatccta acttctacac ccagaaagca gacaccatac
     2581 gtatgatgct cccgttcttt cagcagttcc gggatatggg ggtgaaagac aaagcagccg
     2641 gagtatcagc agaaacggca cagcaacgta taaaagaatt ccactcagaa aaattttttc
     2701 actcactccg gagcacaaca acctttgctg gcagaaaata cacaaacagc gatatgccgt
     2761 cgccgaaaaa aatgaaacta atggcagaca ccatttctgc ggtttatctc gatggatacg
     2821 agggcagaca gtaaggggat ttaccataat cccttaattg tacgtaccgc tgaaacgctc
     2881 tcatacgcgt tctgacgcaa aaacgcaaaa agacaaggaa accccaggct aaaccgaaaa
     2941 aatgcgtcag aatcgttttc aggtacttgc ataactattc acaaaagcga tgtgcgatcg
     3001 atttcgacta ttccgacgga cgaacaggtt aaaatgataa gtttcctccc tccggatgga
     3061 aggttcagca cggggatttt tacccccttc catcaacctg aatatgcatc tgggagtacc
     3121 cattagtttc tactcaaaca ctatcgtagt gctatctcaa aatat
//
LOCUS       DAZUCE010000352         3160 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZUCE010000352 DAZUCE010000000
VERSION     DAZUCE010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.354"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            111..338
                     /locus_tag="ACHY03_004955"
     CDS             111..338
                     /locus_tag="ACHY03_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3908060.1"
                     /translation="MWLPLTDEQRLVILKAYGIECDRLVREKERYEITSISRTQAWKL
                     EREGTFPPRKSIGKKSCGWLLSDLLCWIQTR"
     gene            444..668
                     /locus_tag="ACHY03_004956"
                     /pseudo
     CDS             444..668
                     /locus_tag="ACHY03_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
     gene            921..2354
                     /locus_tag="ACHY03_004957"
     CDS             921..2354
                     /locus_tag="ACHY03_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFS3908061.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVAISALTA
                     FSIACQNQIDVCRPGNLRGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALAEEYA
                     KLVENYSTEKEIWEQKQKALESKLHKEIRAGKDCKATESELKRHLNKCPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGIIFDGRAMSKLGILNSLWDGGSLFIDRKSS
                     PGIILKDPRLTVSVMIQPDVYQKGFCTRKKELVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEQRINELIDENLAMSGERRCLHFSPQAARIWTDYYNDVESKMGAFGP
                     LRHCREYAAKNAEYMARLAGLLHHLSSEEGDISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT          949 a          535 c          722 g          954 t
ORIGIN      
        1 ggtgttgact ggatgaatga tagtgacttt cactcattgt ttatccggga aactaagtca
       61 tttcatgctc atgccggaaa taaaaagtat tgatatatca ggagggtagt atgtggcttc
      121 cattaacaga tgaacagcgt ctggtcattt tgaaagctta tgggattgaa tgcgacagac
      181 tggtacgtga aaaggaacga tatgagatca cgtcaatttc tcgtacacaa gcgtggaaac
      241 ttgaacggga gggaacattt ccaccacgta agtctatcgg aaagaaatct tgcggatggt
      301 tgttaagtga ccttctctgc tggatacaga ccagatgaac tgtctgtatt ttcagtatat
      361 ccagtctgta tagcagctat tgttgctatt gtagcggaaa tagctgctat aaccgaaagt
      421 tattacgaaa aaaggaaata tttatgtctt acatcaaacc ggatactccg tatccagtat
      481 atgcgcaacc atcaatgact ggaaatgcaa taattgaaac tcaacacaat gaaaaagcat
      541 ctctggctat gactaccaca tcattattaa cagcgatgtc aattgcctgt cagaatcaga
      601 ttgacgtgtg caaccccgga aatttacgtg ggcctgttaa tatttacact atggttctgg
      661 ctgattcctg agaaaggaaa aagaccgggg ggaaggtgtt agtgaaataa ttttaggagg
      721 tgttaagaat gagaaacatg aatgatattt cgtggtaaat aacacagtat gaactgaatt
      781 aaccttatcg gtttattaaa catcagatct acagatattc tgtatttctc agaacattag
      841 gtctgaatag cagctatttt cgctattgta gcgggagttg ctgctatttt actaacccat
      901 taataaaaag agaggctttt atgtgcttac ttgcaccaga aaatccttat ccgatatatg
      961 cactaccacc gctggtgaga aatgcaataa ttgaaactca aaaaaataca caggcacctt
     1021 tggctatggt ggcgatatcc gcattaacag cgttctcaat tgcctgtcag aatcagattg
     1081 acgtgtgcag acctggaaat ttacgtgggc ctgttaatct ttactctctg attctggctg
     1141 attccggtga aaggaaaacg accgtggata aagtgtttat gaaagcattt tatctcaggg
     1201 atgaagccct ggcggaagaa tacgcgaaac tggttgagaa ttatagtaca gaaaaggaaa
     1261 tatgggagca aaagcaaaaa gcactggagt caaaattgca taaagaaatt cgcgccggta
     1321 aagattgtaa ggcaacagag tcagagctta aaagacatct gaataaatgt cctgttccgc
     1381 cgcagatacg ccggacaatt ttcaatgaga caacgataga gggaatgtta aaatattatt
     1441 ccgacagcaa tcgctctttt gctcttgtat ccagtgaagg aggaataatt tttgatggcc
     1501 gggcaatgag taaacttgga atacttaact ctttgtggga tggtggttct cttttcatcg
     1561 acaggaaatc atctcccgga attattttga aggatccaag actgacggta tcggtgatga
     1621 ttcagcctga tgtttaccag aaaggttttt gtacgcgaaa aaaagaactt gtgaaaacgt
     1681 caggacatca tgcaaggttt ttgatgtgtc aaccaacatc aacgcagggg acaaggataa
     1741 taaccggaga taattattca tcacagtatc aggatttgtt tgaacagagg atcaatgagc
     1801 ttattgatga aaatctggcg atgagtggtg aacgacgttg ccttcacttt tctcctcagg
     1861 ctgccagaat ctggacggat tattacaacg atgtggaatc gaagatggga gcgtttgggc
     1921 ctttaagaca ttgtcgggaa tatgctgcca aaaatgcaga gtatatggca agactggcag
     1981 gacttcttca ccatttgagc agtgaggagg gggacatttc cccttacact gcagaaatgg
     2041 cgagagaatt agcaatatgg tacggtaatg agtatgtgcg gttgtctaat ccgttaactt
     2101 ttgacaactc tgccctgacc gtacctgtgc gactcattcc agaggagctt gaacttttca
     2161 actggataaa aagctattgt attgagaagg ggatcctctg tatgaaaaaa aatgacattt
     2221 tacagcgtgg tccgaaccgt ttccggaaaa aggataaaat caactggtta ctggatttac
     2281 tgtatgaaca aaacagagtt gtaccggtta ttgagggaaa aacgttgtgt gtcgcaccta
     2341 actttgacct ctgaatgact taatcgttga gtaacatata atctcctctg ctacttgcgg
     2401 aggagactaa tgcctttacc atgtcagcgt tgtattagaa aactctggta caggtcagtc
     2461 agtaaatata actggtgtaa ccactattta ctattggata tcctgttgct caggatattg
     2521 tcggctttag tctgaggtga ggtgggacct tcattgtttt cgtattcaat aaataattta
     2581 ggttaattag tattattaat gggagttatg ttatctgtta tgttgtctta tctctttaca
     2641 tggtaatgtc actcctcttt atgctaagta ttctgtatgt gcgtaataca tatatctcca
     2701 tacactacgc agtgaagcat atggggggga agctgtatgg tgaattatgt ggtgtaatgt
     2761 tgggaacata aatctgttat gttgtcttgt acgcacttaa cactggttac gtaccggaac
     2821 actattagag tatgttatgg ctccgttaca gtttataggt atgaaaaaat ataatctttc
     2881 ctgcatgaaa taatattctt atggtgtgtg gggtatggtc tgtataccat cattttttat
     2941 ggtgaagtta aatatctgta gaggccttaa gctgggtcgc tgtttttact taatggtaca
     3001 gaagtattaa tagcgatgtt gcctgtggct attcgatagc tgacttttgt gaagatcaga
     3061 ttatttcctg taagtaattg aatggtgctt tcttaaaccc aatcatatga ggtgtgtttt
     3121 ttattgtaat tcgttctggt cctcagttta agagagtctt
//
LOCUS       DAZUCE010000353         3127 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZUCE010000353 DAZUCE010000000
VERSION     DAZUCE010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.355"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..273
                     /locus_tag="ACHY03_004958"
     CDS             <1..273
                     /locus_tag="ACHY03_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFS3908062.1"
                     /translation="DFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQYRTASILKNR
                     RVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            276..635
                     /locus_tag="ACHY03_004959"
     CDS             276..635
                     /locus_tag="ACHY03_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908063.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(682..1074)
                     /locus_tag="ACHY03_004960"
     CDS             complement(682..1074)
                     /locus_tag="ACHY03_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908064.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1201..1461
                     /locus_tag="ACHY03_004961"
     CDS             1201..1461
                     /locus_tag="ACHY03_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3908065.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            1458..1895
                     /locus_tag="ACHY03_004962"
     CDS             1458..1895
                     /locus_tag="ACHY03_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS3908066.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            1982..2992
                     /locus_tag="ACHY03_004963"
     CDS             1982..2992
                     /locus_tag="ACHY03_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFS3908067.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEVYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQKIRAAREQWERENGFISNGNGLEAVGTYGGGVFEPMDPEERGCAVETLDCSDWR
                     DD"
     gene            2892..>3127
                     /locus_tag="ACHY03_004964"
     CDS             2892..>3127
                     /locus_tag="ACHY03_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010917792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS3908068.1"
                     /translation="MWELMGEVYSNRWTQKNGAAPSKLWIAQIGAMTEQQIRQVCRQC
                     MDRCRAGETWPPDLAEFVALISESEANPFGLTVDA"
BASE COUNT          883 a          662 c          811 g          771 t
ORIGIN      
        1 gatttttggg cggagaatcc tgatgcagaa caaccgctaa gggcatgggt ggatgaggcg
       61 gcaaaagctg actggaaaag cccagcagac attaaggcac aataccgaac ggccagtata
      121 ttgaaaaacc ggcgcgtggt attcaacata aaaggtaatc attaccgttt aattgttgca
      181 attgcgtatc agcgaggatg ggtatttgtt aaattcatcg gaaaccacaa ggaatacgat
      241 gctattgatg ctgataccat cgaactggag taagcatgaa catcaaacct attcgtacag
      301 agcaagatta tgaagccgcg ttgcgtgctg ttgaacccat gttcgacaat gagcccgaaa
      361 ttgacacgcc tgagggggat ttttttgagg tgatgtgttt gctaatagag gaatatgaaa
      421 aaaaacatta tcccattgag ccaccatccc caattgaagc tataagattt cgcatggaac
      481 agcaggggct gactgtgaaa gatttggaac ccgcaattgg gaaaaaaaat cgggtttatg
      541 aggtgttgaa tggtaccaga agccttacgt taccaatgat tcgccgtctt cataatcaat
      601 ttggtatccc cctggaaagc ctggttggat tataaaatct gctagtcatt tgcctgatgc
      661 tcgttccaga aaaggaatgc atcaggcagt tttgtttttc tgccgcagta actcttcaag
      721 tttccgttta tagaaatcgc gtttttgctc catatcacga atgatctgct ctgcgtcgct
      781 ttgaggtaac tcatctaaaa gcgatatgat ttttcgttgt tgttctgtaa gttgcggttg
      841 gttgtcatta ctggatacag ccattttatc gccgagagtt tcttcttcca taaagaacca
      901 atggacggga tgttgtgaga gctctgctaa tttttccagt ttatccattc ttggcatcac
      961 gcctttcaac caaccttgca cggattgggg ttttacacca agacgtcttc ccagctctga
     1021 ctggtttata ttcaattcct gcaacacctg ctgaaggcgt tttacaaaga tcatcaccac
     1081 ccctcgtaaa ctagttccgc gatcctacag aaaaaattga taagtggcat tacaaataga
     1141 agttgaaatt taaaattaaa tttgtaatca tcggtgtcat cgtaaagttc ggagggaaac
     1201 atgcaaaaaa gtactcaagt gaaaatcctg tcaataatga gccaatcaga attagggcgt
     1261 cgtcttggta aaacaccgca aaccataagt gggtggttta aaaaacgagt gccagcggag
     1321 gaggttatac cagcatgtga ggcgcttgac tggggagtaa ctccgcatga attgcgccct
     1381 gataaatacc ctaatccaac cgatggttta cctgttgagt atcaggctaa cgcacaagca
     1441 gcggcgggag ttgattcatg aaaatcaagc atgagcacat ccgcatggcg atgaatgcgt
     1501 ggctgcttta tccgagggta gggcgcaaaa aaatcgctga tgatatagcg acagcatatt
     1561 ttgagcttga aatgacttat ccaccaatgc atgacacctc tacgacagag ggtattggat
     1621 tgaatataca aaatattttt cgctggcttg aaaaggatac gcctgatgct gttgaaaaaa
     1681 ttcaggcact aattccggct atcctgactg ttcttccgcg tgaactgcgt tatcacctca
     1741 gtatttttga cactgttgag cgccgtgcat tactggcggc gcaggaagcg ttgagcacgg
     1801 caattgatgc acatgatgat gctgtccagg ccgtttaccg gaaagcgtat ttcagcgacg
     1861 gcgggtcatc cggcgagtct gttgtggtgc attgatattt atgccagacc cctgctgatt
     1921 ctgttgattg gggaatcaca gaatatcacc agaggatggt tcgtcacaag atgaggcaat
     1981 tatggccgca ttaccataca tgcaactgta catagctgat tacctggctg acaccatgca
     2041 tttgtccgca gaggagcacg gtgcgtattt gttgctgatg ttcaattact ggcaaacagg
     2101 gaaaccaata cctaaaaaca ggctggcaaa aatttcccgt ctgactaacg agcgatgggc
     2161 tgatgtggaa ccatccttgc gggagttttt ttgcgataac ggcgatgaat gggtgcatct
     2221 tcggattgag gaagatctgg catcagtcag ggaaaaattg accaaaaaat cagccgccgg
     2281 aaaagcatct gttcaggcca gaagaagcag aaaggaagta tatgttcaaa caaaacaaga
     2341 gagagattta acaggtgttc aaacaaatgt tggtgttgtg tttgaacatg acgcaaacac
     2401 aaaagcaact aataaagata cagatctaaa agaattaaac cccacacata acgtgcgtga
     2461 acgcgagagt attccgacca gtgagtcgca tggtgcgccg ttgcagacag ccgaacctga
     2521 atacctggac ggcctgagcg aaccgatcgg gaaattttcg atgaccactg tctggcagcc
     2581 gtcgccggat tttcgacaac gggcagcagt gtggggtatg gctctgcctg agccggaatt
     2641 tacacctgct gagcttgccg cattccggga ttactggatg gcggagggga aggttttcac
     2701 acaggttcag tgggagcaga aatttgcccg ccacgtgcag cacgtcaggg cacaggtaaa
     2761 accagtcagc aagggggtaa gccatgcagc atcaggtggc acggcatcac gggcagttca
     2821 gaaaatccgg gcagcacgag aacagtggga acgtgaaaac ggatttatca gcaacggaaa
     2881 cggcctggaa gctgtgggaa cttatggggg aggtgtattc gaaccgatgg acccagaaga
     2941 acggggctgc gccgtcgaaa ctctggattg ctcagattgg cgcgatgact gaacagcaaa
     3001 tccggcaggt ctgccgtcag tgcatggacc gctgccgggc gggtgaaacg tggcccccgg
     3061 acctggctga gtttgttgcg ctgatttcgg agagtgaggc aaatccattt ggtcttacgg
     3121 tggatgc
//
LOCUS       DAZUCE010000354         3108 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZUCE010000354 DAZUCE010000000
VERSION     DAZUCE010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.356"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            3..614
                     /locus_tag="ACHY03_004965"
     CDS             3..614
                     /locus_tag="ACHY03_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS3908069.1"
                     /translation="MYGTRIIYPAENKEVMVQLMNQGNRSSLLQAWIDDGDTSLPPEK
                     IQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKESIFYLNVLDIPPNSPEQEGKNA
                     LKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNGLVIKNDSANWVTISDVKANNV
                     KVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGNYISDKI"
     gene            630..>3108
                     /locus_tag="ACHY03_004966"
     CDS             630..>3108
                     /locus_tag="ACHY03_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFS3908070.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEVIVKDNPQETCLSREIIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDKNDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHAEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGNRDQFNYGINLSHQHQGNETTAGANL
                     TWTAPAATVNGSYSQSSTYRQVGASVSGGLVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTSYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQCKPWFIKALRSDGQPLTFGYEVNDMHGHNIGVVGQGSQIFIRTNEIPPAVN
                     VAIDKQQGLSCTITFGKEIDESKNYICR"
BASE COUNT          892 a          597 c          791 g          828 t
ORIGIN      
        1 ttatctacgg gacgcgcatt atttacccgg cagaaaataa agaagtgatg gtgcagttga
       61 tgaaccaggg aaaccgttct tcgctgctgc aggcgtggat tgatgatggc gatacgtcat
      121 taccaccaga aaaaattcag gttcctttca tgctaacgcc accagtggca aaaatagggg
      181 caaattccgg gcagcaagta aaaatcaaaa ttatgccgaa taaactgccc actaataaag
      241 aaagcatttt ttatctgaat gttctggata ttccaccaaa tagtccagag caagaaggta
      301 agaatgcact gaagtttgcg atgcaaaaca gaattaagtt gttttaccgg ccagcgggta
      361 ttgctccggt aaataaagcg acatttaaaa aattgctggt aaatcgcagt ggcaatggtt
      421 tggtgataaa aaatgactca gctaattggg tgacgatttc ggatgtcaaa gctaataatg
      481 tcaaagtcaa ttatgaaact attatgattg cccccttaga aagtcagagt gttaatgtca
      541 aaagtaataa tgcaaataac tggcatctga ccatcattga tgaccacggt aactatatca
      601 gtgacaaaat ttaattgcag gagctgccta tgttgagaat gaccccactt gcatcagcaa
      661 tcgtagcgtt attgctcggc attgaagctt atgcagctga agaaaccttt gatacccatt
      721 ttatgatagg tggaatgaaa gaccagcagg ttgcaaatat tcgtcttgat gataatcaac
      781 ccttgccggg gcagtatgac atcgatattt atgtcaataa gcaatggcgc gggaaatatg
      841 aggttattgt taaagacaac ccgcaagaaa catgtttatc aagggaaatt atcaagcggt
      901 taggcattaa tagcgataac ttcgccagcg gtaagcaatg tttaacattt gagcaacttg
      961 ttcagggtgg gagctatacc tgggatatcg gggtttttcg tctcgatttc agtgtcccgc
     1021 aggcctgggt ggaagaactg gaaagtggct atgttccacc ggaaaactgg gagcggggta
     1081 ttaatgcgtt ttatacctct tattatgtga gtcagtatta cagtgactat aaagcgtcgg
     1141 gtaatagtaa gagtacatat gtacgtttta acagcgggtt aaatttactg gggtggcaac
     1201 tgcattctga tgccagtttc agtaaaacaa ataacaatcc aggggtgtgg aaaagcaata
     1261 ccctgtacct ggaacgtgga tttgcccaac ttctcggcac gcttcgcgtg ggtgatatgt
     1321 acacatcaag cgatattttt gattctgttc gcttcagagg tgtgcggttg tttcgtgata
     1381 tgcagatgtt gcctaactcg aaacaaaatt ttacgccacg ggtgcagggg attgctcaga
     1441 gtaacgcgct ggtaactatt gaacagaacg gttttgtggt ttatcagaaa gaggttcctc
     1501 ctggcccatt tgcgattaca gatttgcagt tggccggtgg tggagcagat cttgatgtca
     1561 gcgtgaaaga ggcggacggt tcggtaacca cctatctggt gccttatgca gcggtgccaa
     1621 atatgctgca acccggcgtg tcgaaatatg attttgcggc gggtcgtagc catattgaag
     1681 gggcgagcaa acaaagtgat tttgtccagg cgggttatca gtatggtttt aataacttat
     1741 tgacgctgta tggtggctcg atggtcgcga ataattatta cgcgttcact ttggggactg
     1801 gctggaatac acgcattggt gccatttccg tcgatgccac gaagtcgcat agtaaacaag
     1861 acaacggcga tgtatttgac gggcaaagtt atcaaattgc ctacaacaaa tttgtgagcc
     1921 aaacgtcgac gcgttttggt ctggcggcct ggcgttattc gtcgcgtgat tatcggacat
     1981 ttaatgatca cgtttgggct aacaataaag ataattatcg ccgtgataaa aacgatgtct
     2041 atgacattgc cgattattac cagaatgatt ttggtcgcaa aaatagcttt tccgccaata
     2101 tgagtcagtc attgccagaa ggctggggat ctgtgtcatt aagtacgtta tggcgagatt
     2161 actgggggcg aagcggcagt agtaaagatt atcaactgag ttattccaat aactggcggc
     2221 ggataagcta taccctcgcg gcaagccagg cgtatgacga gaaccacgcc gaagagaaac
     2281 gctttaatat ttttatatcg attccctttg actggggcga tgacgttacg acgccgcggc
     2341 ggcaaatata tatgtctaac tcaacgacct ttgatgatca gggttttgct tcaaacaata
     2401 ccgggttatc gggaaccgtt gggaaccgcg atcagtttaa ctatggtatc aacttgagcc
     2461 atcaacatca gggaaatgaa acgacagctg gggccaattt gacctggacc gcaccggccg
     2521 caacagtgaa tggcagctat agtcagtcga gtacttatcg acaggtcgga gctagtgttt
     2581 cagggggact ggttgcctgg tctggtggcg ttaatctggc gaaccgtctt tccgaaacgt
     2641 ttgctgtgat gaatgcgcca ggaattaaag atgcttatgt caatgggcaa aaatatcgca
     2701 caacaaaccg taatggagtg gtggtatacg acggaatgac atcttatcgg gaaaatcacc
     2761 tgatgctgga tgtgtcgcaa agcgatagcg aagcagaatt gcgcggtaac cggaaaattg
     2821 ccgcacctta tcgcggcgcg gttgtactgg ttaattttga taccgatcag tgcaagccct
     2881 ggtttataaa agcattaaga tccgatggac agccattaac gtttggttat gaagtcaatg
     2941 atatgcatgg tcataacatt ggtgttgtcg gccagggcag tcagatattt attcgcacca
     3001 atgaaatacc gccagcggtt aatgtggcaa ttgataagca acaagggctt tcatgcacaa
     3061 tcaccttcgg taaagagatt gatgaaagta aaaattatat ctgtcggt
//
LOCUS       DAZUCE010000355         3094 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZUCE010000355 DAZUCE010000000
VERSION     DAZUCE010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.357"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            47..1729
                     /gene="ydeN"
                     /locus_tag="ACHY03_004967"
     CDS             47..1729
                     /gene="ydeN"
                     /locus_tag="ACHY03_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS3908071.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLSWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1781..2938
                     /gene="ydeM"
                     /locus_tag="ACHY03_004968"
     CDS             1781..2938
                     /gene="ydeM"
                     /locus_tag="ACHY03_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFS3908072.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2904..3026)
                     /locus_tag="ACHY03_004969"
     CDS             complement(2904..3026)
                     /locus_tag="ACHY03_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908073.1"
                     /translation="MTKHPTGIYVGCLVKAIRRRLRLEQRWSVINYSPFVLQHP"
BASE COUNT          943 a          667 c          633 g          851 t
ORIGIN      
        1 tcgtaaaatt actttggaat taagtacaat aagaagagga acatttatga agtctgcatt
       61 aaagaaaagt gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt
      121 tgctgctcat gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc
      181 agactttacg ccgacagaat acagtaccaa aggaaagcca aatattatcg tactaaccat
      241 ggatgatctt ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat
      301 ggaaaatcgt gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc
      361 acaaaaatca acgccgacgc tcctttcatt aatggatgaa ggcgtacgtt ttactaacgg
      421 ctatgtggca cacggtgttt ccggcccctc ccgtgccgca ataatgaccg gtcgagcgcc
      481 cgcccgcttt ggtgtctatt ccaataccga tgcacaggat ggtattccgc taacagaaac
      541 tttcttgcct gaattattcc agaatcatgg ttattacact gcagcagtag gtaaatggca
      601 cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa caaacacgtg actatcatga
      661 caacttcacc acattttctg cggaagaatg gcaacctcaa aaccgtggct ttgattactt
      721 tatgggattc cacgctgcag gaacggcata ttacaactcc ccttcactgt tcaaaaatcg
      781 tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt
      841 tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt tacctggctt ataatgctcc
      901 gcacctgcca aatgataatc ctgcaccgga tcaatatcag aagcaattta ataccggtag
      961 tcaaacggca gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat
     1021 tctcgaacaa ctgaagaaaa acggacagta tgacaataca attattctct ttacctccga
     1081 taatggtgcg gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag
     1141 tcagacctat cctggcggta ctcacactcc aatgtttatg tggtggaaag gaaaacttca
     1201 acccggtaat tatgacaagc tgatttccgc aatggatttc tacccgacag ctcttgatgc
     1261 agccgatatc agcattccaa aagaccttaa gctggatggc gtttccttgc tgccctggtt
     1321 gcaagataag aaacaaggcg agccacataa aaatctgtcc tggataacct cttattctca
     1381 ctggtttgat gaggaaaata ttccattctg ggataattac cacaaatttg tccgtcatca
     1441 gtcagacgat tacccgcata accccaacac tgaggactta agccaattct cttatacggt
     1501 gagaaataac gattattcgc ttgtctatac agtagaaaac aatcagttag gtctatacaa
     1561 actgacggat ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga
     1621 gatgcaaggc gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaggtaaa
     1681 tcaggagaag tttaacaata tcaagaaagc actaagtgaa gcgaaataac taaaccttca
     1741 tgcggcggaa ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc
     1801 ctccagtttt caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca
     1861 gtttactcat gaaaaatgga tggatgacag cactctgaaa gagttcatca aacaatatat
     1921 cgcagcgtct ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg
     1981 cctggatttt ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat
     2041 ttttaatgca ttacaaacga atggcatttt attgaataat gaatggtgtg cctttctcaa
     2101 agaacatgaa tttctggttg gtatctcgat cgatggaccc caggagctac atgaccgtta
     2161 cagacgcagt aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct
     2221 gaaatcatat caagtagagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta
     2281 ccctcttgag gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga
     2341 attgctcgaa accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg
     2401 catcattgat ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat
     2461 gcaatgggtt aaaaacgatg tgggtgaaat tttcatccgt cagtttgaaa gctttgtcag
     2521 ccgttttttg gggaatgggc ataccagttg tattttccag gagtcctgca aggataatct
     2581 ggttgttgaa agtaatggag acatttacga atgcgaccat tttgtctatc cacagtacaa
     2641 aattggaaac attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca
     2701 aaaaaaacgg atttcagcga aatgtcagca atgtgcatat aaacctatct gcaatggcgg
     2761 ttgccctaag catcgtatta ctaaagtaaa cgatgagact gtttcctatt tttgcgaagg
     2821 ttataaaatc cttttttcaa ccatggtacc ttatatgaac gccatggtgg agttagccaa
     2881 gaatagagta ccgctttacc acattatgga tgttgcaaaa caaatggaga ataattaata
     2941 acgctccatc tttgctccag tcttagcctc cttcttatgg ccttaacaag acacccgaca
     3001 taaattcctg tagggtgttt cgtcatcatc atcttccctt gcgaaaattc tggcttacgt
     3061 ggtatgcgtc agggcattag caataccctt gatt
//
LOCUS       DAZUCE010000356         3088 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZUCE010000356 DAZUCE010000000
VERSION     DAZUCE010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.358"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            122..355
                     /locus_tag="ACHY03_004970"
     CDS             122..355
                     /locus_tag="ACHY03_004970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908074.1"
                     /translation="MFKDDDFSNIKLPRYSSTSGNSIDVKCDIRGIGSDILFTASKTD
                     STLYGRNLYERALAGEFGDVAEYVAPIIIESGE"
     gene            361..1854
                     /locus_tag="ACHY03_004971"
     CDS             361..1854
                     /locus_tag="ACHY03_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HFS3908075.1"
                     /translation="MSTNFLHGPRTLEYDDGTKEINTVDISVIGIVGTAPDVGLGERA
                     SLTWGSPLANNVVTFFPVYYGDYGNKFAVEIIKKGISGIKYGYSTLPDGTIKLTLGTD
                     STTTPERLAQDVVTHGEGRTAEDCICVGIDEDSKNDGIVYQMPVTSLTGGSDAPFPVN
                     IPTLIAGSQKKAALLGAAGTLPAAITDILNQTDALIVVVRVDENTDATKERENVINGI
                     NVLLTSGQINQVTPRILIAPDYSANDDVAAALEVVTNKLRGVGYIDSPRTASPADVVN
                     RRHKYSARMEILRPRVFSTGDLSDLSRPYSAIAAGLRARIDNEKGFWWSKSNQNIYGI
                     TGLEQVDDFIIGETNCTANLLNASQVSTIIRYDGFRHWGNYLCSLDPQWSFECVRRTA
                     DVIEDSIARAMMTDFIDRPIDLHLGTDVVESINAYLHKLEEQGAINGGRAWLDGELNT
                     KESLAAGNLYINVDFGPKSPAQTITLMYRINNDYTVEALASLFKETA"
     gene            1872..2384
                     /locus_tag="ACHY03_004972"
     CDS             1872..2384
                     /locus_tag="ACHY03_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFS3908076.1"
                     /translation="MSDSNAYRAFALFVQGERVLNCTEYTPVDMKIIEDEFKTGAMDT
                     AITLDGGMEKMSASFKVSGSDSGVMSYFGLIPGVKTRFEIRSAYTDSYGINFERIDTY
                     EGLITAITDDAQGTDSKSSVGQSVTIAPSYYKRVQNGRIIYEIHPAKMKRVINGVDVL
                     AGVARILHVY"
     gene            2397..2678
                     /locus_tag="ACHY03_004973"
     CDS             2397..2678
                     /locus_tag="ACHY03_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFS3908077.1"
                     /translation="MESLLDSMTITLSRPFIIKGESCDTITIREPKLRDRIMFSNDKS
                     GLEERTATMLARLANLEREDLYALPACDYDQMEAAFNELVKPPKDRHQI"
     gene            2787..>3088
                     /locus_tag="ACHY03_004974"
     CDS             2787..>3088
                     /locus_tag="ACHY03_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908078.1"
                     /translation="MAISQNFKTQVVFGGRIDPSFRRGTTELNDAIRQTSSTVGKLTK
                     SQDKLKDKIAAMKLAGKDVSDLSAQYQKLDRRIKATTQDQEALNTQLAKKQRLENWT"
BASE COUNT          917 a          625 c          757 g          789 t
ORIGIN      
        1 tgcctggctt gtgggcgctc tcagggtata tgccgtcgag ttccgaaccg acagacggta
       61 atgccgaagc tatttttaca gcgagttttg caacaaattt cattagagta gcctaaataa
      121 aatgtttaaa gatgatgatt tttcaaatat aaaattaccg cgctattcta gcacatcagg
      181 caatagtatt gatgttaaat gtgatatcag aggtatcggt agtgatattt tatttactgc
      241 atctaaaacc gattccacat tatatggaag gaatttatac gagcgagctt tggccggtga
      301 atttggtgat gtcgctgaat atgtggctcc aattataatc gaatcaggag agtaaaagaa
      361 atgagcacta attttttaca tgggcctcgc acgctcgaat acgacgacgg cacaaaagag
      421 atcaacacag tcgatatttc agttatcggg attgttggta ctgcaccgga tgttgggcta
      481 ggggagaggg catcattaac gtggggatca ccgctggcaa ataatgtagt cacctttttc
      541 ccggtctatt atggggatta cggaaataaa tttgcagttg aaattattaa gaagggtatt
      601 tctggaatta aatacggata tagcacactt ccagacggaa caataaaatt aacgctcggg
      661 accgacagca caacaacgcc ggaacggctg gcgcaagacg tagtgactca cggtgaaggg
      721 cgaaccgctg aggattgtat ttgtgtaggc attgacgagg acagtaaaaa tgacggaatt
      781 gtttatcaga tgcccgtcac gagcctgacg ggcggttctg acgctccatt tccagtcaac
      841 atacccacac taattgcagg tagtcagaaa aaagcggcgc tcctgggtgc agcaggcact
      901 ctccccgcag ccatcactga tattttaaat cagacagatg cgctaattgt tgttgttcgc
      961 gtagacgaaa acacagacgc gacaaaagag cgtgagaacg taatcaacgg aattaacgtt
     1021 ttgttaacat cgggacaaat aaaccaggtg actccacgaa ttttgattgc acctgattac
     1081 agcgctaatg acgacgtcgc tgccgcgctg gaggtagtca caaataaatt acgtggagtc
     1141 ggctatattg attctccacg cacggcatcg cccgcagacg tcgttaaccg ccgccataaa
     1201 tatagcgcgc gcatggaaat actacgcccg cgtgtgtttt ccacgggcga tctcagtgat
     1261 ctgtcgcgtc catattcagc tatcgccgct ggtttacgag ccagaattga caacgaaaaa
     1321 gggttttggt ggagcaaatc taatcaaaat atttatggaa taacagggct tgagcaggtt
     1381 gatgatttca ttattggcga gacaaactgc acggcgaacc tgctgaacgc tagtcaggta
     1441 agcaccatca ttcgctatga tggattccga cattggggta actatctgtg cagcctggat
     1501 ccgcagtggt cgtttgaatg cgttcgtcgg acagctgacg taattgaaga ttctatagcc
     1561 cgggcaatga tgacagattt tattgatcgc ccgatagacc tgcatctcgg aacagacgtt
     1621 gtcgagtcga taaacgcata tctgcacaaa ttagaggagc agggtgcgat taacggaggg
     1681 cgtgcgtggt tagacggtga gttgaataca aaagaaagtc tggccgctgg taatctttat
     1741 attaatgttg attttggtcc gaaatctccg gcacaaacaa ttacgttaat gtaccgcatt
     1801 aataatgact atacagttga agcgctggcc tcccttttta aagaaacagc ctgataattc
     1861 ggagagtaaa tatgtcagat agcaacgctt accgcgcgtt tgcgctattc gtgcagggtg
     1921 aacgggtttt aaattgtact gaatatactc cagtcgatat gaaaataatc gaagacgagt
     1981 ttaaaacagg cgcgatggat acagccatca ccttggatgg tggtatggag aaaatgtcag
     2041 caagttttaa agtttcaggt tctgattctg gtgttatgag ctatttcggg ttaataccgg
     2101 gagtcaagac gcggtttgaa attcgtagtg cgtacacaga ctcatatgga ataaattttg
     2161 agcgtattga tacctacgag ggacttatta cagcaatcac tgatgacgct caggggactg
     2221 attcaaaatc gtcagtgggg caatcagtaa cgattgcgcc gagctattat aaacgagttc
     2281 aaaacggacg aattatttac gaaattcatc ctgcaaaaat gaagcgcgtt attaacggtg
     2341 ttgatgttct tgcgggcgtc gctcgaatcc ttcatgtata ttaaaaggca aataaaatgg
     2401 aatcattatt agacagtatg actattacgc tctctcgccc gtttattatt aaaggcgaga
     2461 gctgcgatac aattactatt cgcgaaccaa aattacgcga tcgtattatg ttcagtaacg
     2521 ataaaagtgg tcttgaagag cggacagcga caatgctggc acgcttggca aatctggagc
     2581 gagaggattt atatgcactg cctgcgtgcg attacgatca aatggaggcc gcgtttaacg
     2641 aactggtaaa gcccccgaaa gaccgacatc agatatagtt attctgattc cgtttattgc
     2701 gaaaaaactt gctatcccgc ccgatacgca aatggacctg ccgtatcggg tttttaattt
     2761 ctatgtaaac gaggtcatga aaaccgatgg ctatttctca aaactttaaa acgcaggttg
     2821 tatttggggg gcgaatagac ccgtcgtttc ggcgcggtac aacagaactc aacgacgcca
     2881 ttcggcagac gtcgtctact gtcggaaaat taacgaaaag ccaggataaa ttaaaagaca
     2941 aaatcgcagc aatgaaactg gcgggtaaag acgtttctga tttatctgct cagtatcaaa
     3001 agctggaccg tcggataaaa gcgacaacac aggaccagga agctctgaat actcagctcg
     3061 ccaaaaaaca gcggctggag aattggac
//
LOCUS       DAZUCE010000357         3076 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZUCE010000357 DAZUCE010000000
VERSION     DAZUCE010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.359"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(272..1750)
                     /gene="ygcE"
                     /locus_tag="ACHY03_004975"
     CDS             complement(272..1750)
                     /gene="ygcE"
                     /locus_tag="ACHY03_004975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFS3908079.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGQITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPETLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYTWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKALTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            complement(1777..3054)
                     /gene="yqcE"
                     /locus_tag="ACHY03_004976"
     CDS             complement(1777..3054)
                     /gene="yqcE"
                     /locus_tag="ACHY03_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFS3908080.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADIAPTTANSK"
BASE COUNT          810 a          786 c          698 g          782 t
ORIGIN      
        1 ataaagatat ccagttcata ctttaagtga aaattgataa agtgcgattc gtattgtctt
       61 ttattctaaa gacatcgagt gtagttaata ttccttgtaa aaacagggat aaaccgaact
      121 agttaaagtt tttattatgt tccctgcatc agcggggata aaccggactt gttatcattt
      181 tttaagttgt cgaacttatg ttccccgcgc cagcggggat aaccattcag gtatgttcag
      241 gccgggctgt ttcccgcccg gccttcctca cttacgcatt cgaccagctc tggatcgaat
      301 ccgcattccc caattcccca tgcatcactt catacgattt tttcaggatc acatcagtat
      361 gtttggttag ggctttgaaa atacctttat tcatcgcgtc gtagcgtttg gcattgctct
      421 caacaggcat aaagatatct ttcacgcgaa ccattttgtc gacagccgtt gcgtaatccg
      481 ggtatagccc cagacctacc gccgtattga tcgctgcccc cagacttgca caaccgttaa
      541 tggcgttacg gcgtgccgga aggttgaaca cgtcggcaaa aatttgcata aatagatcgc
      601 tgttcgaacc gccgccagta atgatcacat gctttgcaaa gtgattcatt tcattacaca
      661 tattgtcgta attgttcttc agcgtcagcg ccacgctttc cagtatcgaa cgatatatcc
      721 atgtgtaatc catgctggaa tcaaagccga tcataatccc acgtttgtag ggttcccacg
      781 ggttggtcag ccagtccagc accgtcatca gaccattaca gccaggcggc acacaagaag
      841 cttttttgtt gagcaaatct tccggtgaaa gatcctgtgc tttggcatcc tgaattaacg
      901 actcgcctaa catgtcgcgt agccagctca ccgtccacat gcctttacgg ataccgtacc
      961 cttcatacag caatgtttcc ggtatagaag acataatcgg ccagtacgcc accggatctt
     1021 tcggcagtgc tttaccgttc atcatcagcg cgatgtaggt gccaagagaa atcaccgccg
     1081 tttcatcatc cagtaatccg gctcccagag cttctaccgg tttatcactg gtggtacaaa
     1141 caaccggcag ccctgccggg aaatgtgtcg ccagcgctgc ttgaggtgtg atctgtccaa
     1201 gaacagtgcc tggcatttgc acatcaaaca gcatatgacg ggggatatta aacttctcca
     1261 tgactgcggc atcttcactc catgcccagc tcttataatc caccggccac tgaccaaaat
     1321 agttggcgat attgtcttta aactcgccgg ttaagcgatg cgtcagataa cccgaaaaag
     1381 aggtgacata tgccacgtca ggattagtgt gttcgtaggg acgcgttacg cgtgcatcct
     1441 gccagctaat caatggcgca gctggcgtgc catcggcttt caataacgca cggcagcaac
     1501 ggatggatcc cagaccaata ccgacaatat cttctttatt cccggcaaac tgactcatca
     1561 aatcgtgacc ggcaaaacat aatgatgccc ataaatcgtc gtcaggatgt tctgcggtat
     1621 cggcatctgg cgtgtgcatc ggctgcaata aacctttgcc ttcgcaaacc acgttacctt
     1681 ccagatcgta catcaccact tttgtgctct gacttccacc atcaatccct atgatgtatt
     1741 tcttcgacat gatttttctc ctgtaatttt tctggcttac ttgctgttag ccgttgtggg
     1801 ggcgatatca gcagtacgaa ttttttggaa cagcaaaaag gtaaagacga tcaccatgcc
     1861 gagcgccgcc atgcccatca gccacatatt gcggtaggct tctgctgcgg gcagggtgtc
     1921 ttgccagtgg ccgataattg ggtaaacgaa gacatccggc aggaatccta tcaccgaaca
     1981 aatacctacc gtggtgccca taatgtaaga cggtgttctt gcttcgccag ggcaggccca
     2041 atacagcccg cgtgaggcgt aacaggtgaa tcccagcagt aaaatcaggc caatccccat
     2101 cgcgaccgat tgcgggttag agttcgtgac gagcagggca gttaacgcca gcaggccgag
     2161 tacggaaagg atttggatca cgcgggtagg ggatttcact ttactgtagg tggtgataat
     2221 gccgccaagt gggccgcaca gcgcgcggaa gattttgttg atcacaatcc ccatgtagct
     2281 cgccgccacc agcgacatgc catacatttc ggtcagatag ttggtggagt aactcagaat
     2341 ggcgtagatg gtgaagacgc caaaaatcac catgctgcaa taccaggtgg tgctgatacg
     2401 caaaacggca aggatgtcgc taagctggaa tgactgtttt tcttcgttat tggcactgcg
     2461 caggttgttg ttatcgctga caaaaaacca gcacagaatc cccaacaaga tgtaaaccac
     2521 actatagatg ataatgaccg ttttcaggct ggtgctgtca tccggtgcaa agcgagaaaa
     2581 aacccacatg gtaaacactg ccagcgacat tacaccgacg ccgcgcagcc cttccatcca
     2641 gcccataatt ttcccttgct cgctatgatc gccaagcaac gatgcggctt taatcgacac
     2701 cgaccacagc attaaaatcg ttgttatcgc aaaggcgacc tgaatacaga gcattaccca
     2761 cagcggtgga tacgttgcca ttaacagacc cagtaatccg gtaatgatca tcgcggaagt
     2821 aatcattttg cggtgtgaaa atttatcggc aataacgccg ctgggggcat aaagaataat
     2881 agccgcaata ccaaaggtac tcattattaa acctatctcg gtattgctga atcccataaa
     2941 tttcgccatg ggaatttgat aaatataacg taaataagcc aggtcgaaac taacgccgcc
     3001 gctaaaacta attatcgcga gggttatcca acggcgatat gagttgtgtt gcatatcgta
     3061 ttcccatttg taatag
//
LOCUS       DAZUCE010000358         3066 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZUCE010000358 DAZUCE010000000
VERSION     DAZUCE010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.360"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..481)
                     /locus_tag="ACHY03_004977"
     CDS             complement(<1..481)
                     /locus_tag="ACHY03_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0046903 - secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrpJ domain-containing protein"
                     /protein_id="HFS3908081.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALTQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     "
     gene            complement(486..2189)
                     /gene="sctC"
                     /locus_tag="ACHY03_004978"
     CDS             complement(486..2189)
                     /gene="sctC"
                     /locus_tag="ACHY03_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system outer membrane ring
                     subunit SctC"
                     /protein_id="HFS3908082.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(2186..2935)
                     /locus_tag="ACHY03_004979"
     CDS             complement(2186..2935)
                     /locus_tag="ACHY03_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908083.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIG
                     NNHQKLMVVEDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHE
                     LFADALPFASEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQS
                     LIQTSLYDLGELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTI
                     AHKYGYSSSSHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          864 a          671 c          508 g         1023 t
ORIGIN      
        1 taatgaattt tttagtagtc tcttgattaa cttttctaag taatgcttca gcgtttagcc
       61 ggacattttc tgatatttgt tttttacgta aaattgcacg taacacaact accagatcgc
      121 tttcatctgg gaataatttt ttagcaaagc tatacacttc atagtcatgg gtagttgcag
      181 ccttaagcgc ctccataact ttgtcggttt tttctattgg ctggtcttcc aggacatatt
      241 cttctccgtc cagaaaattg gccagttgtt cttcgtagat ttttttcttc ataaactgcg
      301 ttaacgctgc tgacatctcg tcagtagctt gtaagaaccg agcttcttta cctgcgggat
      361 catcccccat tttaggcatt tcagcttcaa tatcttcatt atcttctgtg ataatgtcct
      421 ctaagcgaga aacttccctg gctctttcta atacaccgac atgctctaca tgaatcgcca
      481 tttcactatc ctccgacgac ttcacgattc aggtaagttt ttatccattt ctgcagtaat
      541 tcatcgttaa tttctttatt ttttgccatt tgctgtgtaa tcgctctggc atcaaccgca
      601 gcctcattcg cattattcat catgcgctca tcgatctctc ttggctcaat aagaaagaca
      661 cgaacgatgt tattcgcatt tgttccttca taaccaaata atttaccaat aaatggaatg
      721 ctgcctaata ttgggatctt gcgactttca taggtattcg tatcgcgtgt atatccccca
      781 atcaaaagac ttttcccttg gggcacgcgt gcaatagtac ttataagtgt tctaccaacc
      841 tgaggcagat catcaacatt ggtcttgtcg gaattgattt catttccatc ttcaatattc
      901 aagagtaatt caatttgatt tcgcgcagca aatcgtggta gcacgctaat catcgtacca
      961 taggttactt catccaattc cgcagtacgc tcgccgacta atttagtata gaaagtgcgg
     1021 ttattatcaa atatagcagg aatattctcc tgtgttaaaa cgacgggacg agcaacaacg
     1081 gcggctcttc ttttttgtgc aagtgcctgt attgttgcaa taaactgtgt cccatcaagg
     1141 gtactaattg aacctgaatt attaaatgat gcacttaagc tggatccaat ttttatcgtg
     1201 cccgaccagt cagcacctag ctgttctaga tcagtcttat caatatctat tatccataaa
     1261 gataattcaa tgtgtcgctt tggaatatca agcgtagcta ctaacttctc gataaaatca
     1321 acctgagata ctgttccttt aacaagtaaa ctgttggtat caggatatgc aataatttgt
     1381 atatcgtcag tctctggcgc tcctgctgtc gatattattt tctctacttg taggtcatca
     1441 tcttcttcag ccccaggaaa aggaggcata cgtgatacgt tttttagttg ttgacgactt
     1501 gaaatttcag aaagtacgtt cacccctgtg ctttgcttaa tgttattatt taacaacgtc
     1561 gatagtactt ttgccatgcc aggaataact atcttttcgc cacgtaactc ataagtccgg
     1621 tcattaacga atgtgttgac cagatgaatt attcctactt tatttcgacc gatctcaatg
     1681 ccatcgctgt tttgctccat taatttcgca gcattaacga ccaggtcaac ataaaccggt
     1741 ggccccgata catagaaagt accattgccg ccccctttaa tttcataacg agagttatac
     1801 agacccgatt tttttaagaa attattaaac tcattcgtcg atacctttct cagattaatc
     1861 aatgcattac gcatctctga tgagtcgtag atataaatag ctttgccatc atcataccaa
     1921 ataagcccca tctgtgccga caggcgttca atcaaggcat agggttctgt taaatcaaaa
     1981 tttccgctga tctgtttctt catggcaagc ttactaacta ttgtaggctt acccgcatat
     2041 gatgaaaccg tttcaaaaaa actacgtaga ttttctttat ttgctacgta tccattcgct
     2101 gctgcggcat atgcaccagg agtcaataat ccattaaaaa tgcataagac tgaaattaat
     2161 ataatagtaa tgcgtaattt tattttcata actttttaca tagttctctc ggtgtttttc
     2221 ctaaccggct ttttacttcg gctgaaaagt gtgatgagga cgagtaacca tatttatgcg
     2281 caatagttgt catatcactg tttccttcaa tgatttccaa cacggctctg gcaactctcc
     2341 agccacataa ttccgtttta actttaccac ccaatgcata actacataaa cggcgaaaat
     2401 gtgaatacga tacgccgtaa agctcaccaa gatcatagag tgaagtctgt atgagcgatt
     2461 gcgataataa ataacgaacc agtttatagc attctgtttt tcttagatgc tgaaacagga
     2521 tttcattaat attatcgctg ctcagataag tggctaaaaa ccaggcttca cttgcaactt
     2581 tactgaatag cacaacttct gatgcaaagg gcaaggcatc agcaaacaat tcatgatgtt
     2641 ccctttccac actccccgtt gattcatcta taaaagctaa taatttactc aggatatcaa
     2701 tgcgaagtgg atgataaatt actttccctg cagttacctc aatttggctt cggtcaatga
     2761 gcagtaatat atcttccact accattaatt tttgatggtt attaccaatc gtaataacaa
     2821 gggttacatc cttgttttca tttccccgta agaaccacat ttcagggtcc agtagggtta
     2881 acttctgtcc ttgaagcaaa acattcattg gaagtaacag cccttcttca atcatgtttc
     2941 atctccttat tccatattct cattctccat aaacttgcta taaaaacatg attaaaaagt
     3001 gatgattact atttataaaa taatgatttt gttatgagcg aatagctatc agccacttac
     3061 actagt
//
LOCUS       DAZUCE010000359         2979 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZUCE010000359 DAZUCE010000000
VERSION     DAZUCE010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.361"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..139
                     /locus_tag="ACHY03_004980"
     CDS             <1..139
                     /locus_tag="ACHY03_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFS3908084.1"
                     /translation="RVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHESNSYTVGMG
                     V"
     gene            complement(305..1213)
                     /locus_tag="ACHY03_004981"
     CDS             complement(305..1213)
                     /locus_tag="ACHY03_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3908085.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAELVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRTGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            1442..1753
                     /locus_tag="ACHY03_004982"
     CDS             1442..1753
                     /locus_tag="ACHY03_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3908086.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1754..2044
                     /gene="nadS"
                     /locus_tag="ACHY03_004983"
     CDS             1754..2044
                     /gene="nadS"
                     /locus_tag="ACHY03_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFS3908087.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            2628..2846
                     /gene="yiiE"
                     /locus_tag="ACHY03_004984"
     CDS             2628..2846
                     /gene="yiiE"
                     /locus_tag="ACHY03_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFS3908088.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
BASE COUNT          839 a          641 c          723 g          776 t
ORIGIN      
        1 tcgagttctt catgtctcta acaaatttaa tgttgggttg caaggtaata tcaacacgga
       61 tgttttacaa gattcgtttt ttaaaaatgt cgatttcatt catgaatcta actcgtatac
      121 cgtagggatg ggagtctgac gagtaaaact cattaggatt atcagaattc ggccaatgca
      181 ttgcaaaaat gaaatacaga gagaagtgtg caggcgctta gccgcgaggg gcatggcgaa
      241 gccatacaga aagcaccagc attcagaact cctgtgaaag cggcatagtg ccgctgctca
      301 ggagttacct tcccgctctc caggccacat cccgcagcgc cgatcgcgca gcctcactga
      361 taccacctaa ctgaaaaaat ccgtggataa cgccaaggta gcgttggcag gtgcattgta
      421 ctccttgccc tgtcatgcgg tgatacaacg cttcaccttc atcgcaaagc gggtcgtatt
      481 cagcggtaat gatatgtaca ggtggcaagc cattgaaatc atcgcgccaa agagggctgg
      541 cttcaggatg cctgcgttca atgcctggca gatacatctc aaagccgctc agtaaagtgt
      601 cgcgtgtgat gatgtaatcg tgaccattgc gtatgtagct ttcgaaatgc gcagtcgcat
      661 caagcatggg ataaattaag atgagttgtg ccggttgcca ctcgccagta cgctttaatc
      721 gcaaagcagt taccagcgcc agatgtccgc ccgcactgtc accggcaaga gttatccgtt
      781 gtttatcaac gccgagtcgt tcggcgtgtt gccagactaa ttctgcacct cgttgcgcat
      841 catcatgggc ggcggggaaa gtatgttccg gtgccagccg atactggacg gcaataacac
      901 gacattgccc gtaataggct aactggcgca gctggttgtc gtgagttgcg aacccgccgc
      961 taacaaaaca gccaccgtag taataaatta tcgtcgggag taatgtcggg gcattaagcg
     1021 gcgacactat tcgtaaggtc atcccttcaa gttcgatagt ctgaatatct acccgcgttt
     1081 ctgtctcacc cgcaaggaca gcgctggcga tatagccctc cctgcgctgc gcaatacttt
     1141 gttgacgcga tgaggggcga cccgcagcga taaactcttc gactaactct gcaattcctt
     1201 tttccagtgc catagtagaa ctcattttgc tgtatgaatg tacagtttta tacacccgtt
     1261 tttgctggat ggataccggg aaattcacgg gcgtgaaaaa tctggaaagc gcctcgcaaa
     1321 tgtaaagaca aacccggcag agacgtggtt ttgctggagc actatcagta agctagtggt
     1381 ttcaagggta cgtcaatgac gtacaaaaag gattgttact ctaagccaat ggcgtataat
     1441 tatgctcttc atcgaaacgg aaatttttac tgaagatgtt caaaaactgc tgaccgacga
     1501 tgaattcagt cgttttcagt ttttcctggc cttgaatcct gactatggtg aggtaatacc
     1561 tgaaactggt gggttaagaa aggttcgatg ggtgtcagga ggaaagggga aacgtgctgg
     1621 tgtcagggtt atttactttc atcaggttaa acattatgag atcagattac tgcttattta
     1681 tcgtaagggt attaaagatg atctgtctcc ccaggaaaaa gcaatgctcc ggttgttaaa
     1741 tacgaggtgg taaatggata aggcactttt tgagcggttg actcacagca tggcccagat
     1801 gaatgaaatt attgaaggaa cgcgacagcc ttcgcggact tttgaagttg atgctatgaa
     1861 aatcaaagaa atacgccgcg cttcagggtt gtcacaatct aaatttgccg atcttatttc
     1921 tgtaagcgta gacacgttgc gtaactggga gcaggggcga cgttcaccga ccgggcctgc
     1981 aaaggctttg ctgcgagcga ttgccaatga tccgcaacat gtgctgcaag cacttaaccg
     2041 ttagaggcat ggttatgcca tacagaaagc actagcattc agaactcctg tgaaagcggc
     2101 atagtgccgc tgctcaggag ttttgttatg acccgtatcg tgctgagcgt tgccacccta
     2161 tgatgcgatt aaaaaaactc accgtcgagc cgatgattga cgtcaatgct atcggatgat
     2221 gccgggggtg ttccggaagg aattccttgt ttcccgaact gttaccaaca aaattataac
     2281 gatgagcaag taaagaccgg ttggtggcag tactgaccgg tctgtgatgg aagattaatt
     2341 tcaccttaaa tcctggtgct gttaggatgc tgacgttttt cggctacacc attcagatta
     2401 tgcaaataaa tatccctgaa tataatatat tcatagcacc cataactcat cctgggcgat
     2461 aactaaacca tttttgagta acaaatgaaa caactgcaac gctcttatta tttctggaaa
     2521 acgtctcgca aaaacaaaac aacatgcaaa aatccgcttt cgttaattaa ttttccggcg
     2581 taattttcgc acatacatta atgagtaata tatgtaagag ggtggcaatg gcgatgaata
     2641 cggtttttct tcatttatca gaagaggcaa ttaaacggct gaacaagctt cggggctggc
     2701 gtaaggtttc acgatctgca attttacgcg aagcggtaga gcaatatctg gaacgtcagc
     2761 aattcccggt gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg
     2821 atcagtgtaa ggagcataag gaatgacaaa aatttttcat tcttgaatat aaaaaacaga
     2881 tgcccttatt ctggtattaa tacaaggctg ttttacttga acttataata actgcaactg
     2941 ttacatcata tctggaaaac gcctcgcaaa aataaaaaa
//
LOCUS       DAZUCE010000360         2972 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZUCE010000360 DAZUCE010000000
VERSION     DAZUCE010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.362"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            18..266
                     /gene="ymgC"
                     /locus_tag="ACHY03_004985"
     CDS             18..266
                     /gene="ymgC"
                     /locus_tag="ACHY03_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFS3908089.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            598..2121
                     /gene="pdeG"
                     /locus_tag="ACHY03_004986"
     CDS             598..2121
                     /gene="pdeG"
                     /locus_tag="ACHY03_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS3908090.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            2253..2471
                     /gene="ymgF"
                     /locus_tag="ACHY03_004987"
     CDS             2253..2471
                     /gene="ymgF"
                     /locus_tag="ACHY03_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFS3908091.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
BASE COUNT          801 a          600 c          639 g          932 t
ORIGIN      
        1 atttctacga ggctgacatg aataattcaa tcccagagag atttattttt caatgtgctt
       61 tgtttaaaaa tctcgagagg gaggtgttca tgacgcatgg gtatgttgat agtcatatta
      121 ttgatcaggc tttacgcttg cgtttaaaag atgaaaccag tgtgattctt tctgatctct
      181 atctgcaaat attgcagtac attgaaatgc ataagactac actaacggat atcattatta
      241 atgacaggga atccgtgctc tcttagaact tgagtaaggt tttagaataa acatcagctg
      301 tatcaccatg ctgatgcaaa gtgagcattt caggcgttat gctttcttat tatgtccgca
      361 atatcaggtg tcaagaatgg agagttctcg ctctccattc ttgacgcctg atatcccgcc
      421 taacttattt tgtactgcct acattatgct caataattaa tgggttagat caagatggag
      481 aaaattttat cttcggcgtg gatatttctt atgctctcta ccatgctctc tgtagatgcc
      541 agcgcgagcg aatgaatggc attttcatgt attaaaccct ttggccggga caagcaaatg
      601 cgcaatacac tcatacccat cctcgttgcg atatgcttgt ttattaccgg tgtcgctatt
      661 ttaaacatcc agctctggta ttccgcaaaa gcagagtacc tggcgggagc gagatatgcc
      721 gccaacaata tcaatcatat acttgaagaa gcgtcacagg cgactcaaac agcggttaac
      781 attgccggga aggaatgcga cctcgaggag caatatcagc ttggcactga agcagctctg
      841 aaacctcacc tgcgcacaat catcattctc aaacagggaa tagtctggtg tacatccctg
      901 cctgggaatc gggtcctgtt gtctcgtatt cctgttttcc cggacagtaa tttactgttg
      961 gctccggcaa tcgacaccgt taatagatta cctatcctgc tctatcagag ccaatttgca
     1021 gatacgcgca ttttggttac gataagcgat cagcatattc gcggggcgct taatgtaccc
     1081 ttgaaagggg taaggtatgt attacgcgtg gcggatgaca ttattggacc tacgggtgat
     1141 gtgatgacgc ttaatggaca ttatccctat accgagaagg ttcactccac aaaatatcat
     1201 ttcactatta tctttaaccc gccaccactc tttagcttct acagacttat cgataaaggc
     1261 tttgggattt tgatatttat tctgttaatc gcctgcgccg ctgctttcct gcttgatagg
     1321 tatttcaata aaagcgcaac gcctgaagag atcctgcgac gggctataaa taatggggag
     1381 atcgtccctt tttaccaacc tgtggtaaat ggtcgggaag ggacattgcg gggagttgag
     1441 gtgttagccc gctggaaaca acctcacggt ggatatattt cacccgcggc atttattcca
     1501 cttgctgaaa aatccggatt aatcgttccg cttacacaaa gcctgatgaa tcaggttgcc
     1561 agacagatga acgctatcgc gagtaaactg ccggaaggtt ttcatattgg aattaatttt
     1621 agcgcttcgc atattatttc gccgacgttt gtcgacgagt gcttaaattt ccgtgacagt
     1681 tttacccgcc gcgatttaaa ccttgttctg gaagtcaccg agcgtgagcc attgaatgtt
     1741 gatgaaagtc tggttcagcg gttgaacatt ctgcatgaaa atggttttgt catcgcgctg
     1801 gatgatttcg gtactggcta ctcagggctt tcttatcttc atgacctgca tattgattat
     1861 atcaaaattg atcacagttt cgttggccgc gtaaacgcag acccagaatc cacccgaatt
     1921 ctggattgtg tattggatct ggcgcgtaaa ctttcgatca gtatcgtcgc tgaaggtgtc
     1981 gaaacgaaag aacaacttga ctatctgaac caaaataata tcacatttca gcagggttat
     2041 tatttctata aacctgttac atacatcgac ctggtcaaaa ttatcctttc taaaccgaag
     2101 gtgaaggttg tggttgagtg aaaattgatc agtaagacca tagtgcggtg taattataga
     2161 cagctaatta gctcgttgcc tcttgttact attgttcatt attttgtttg ctataattgt
     2221 ttgaaagttt tgacaggatt gccattagta gcatgaacaa tagtaataat ctggattatt
     2281 tcactctcta tatcatcttt tccattgcat ttatgctgat caccctcctg gtcatcctta
     2341 ttgcaaaacc cagtaccggg ctgggagaag tgcttgtgac gataaatttg cttaatgccc
     2401 ttgtttggct ggcgatcaat ctggttaatc gattaagaga aagactcgtc agccacaggg
     2461 atcagcaata atctttcagt ttctcactgt cagtatgcgg ctgaatgggt tgctggcagt
     2521 gaacgcctgg atcattgaag gaaaggcatt attgcgcaaa tagttgtcaa ccctggtgtt
     2581 atcacggttg tttttatata tcaccgaaat aatcctcatc gcaactatta acaattttga
     2641 tgtcgaagag ttatttgtta aacaaaatcg tcaccctaaa gtgatcaatg tcatgaaaat
     2701 aaggtgaaaa atgataatgc cgacttattt atcatttata tattgtcgct gtttatctat
     2761 ttaaattttt tgtgcttttg tttttgtctt taccctccgt tttggtaatc tgaacgcata
     2821 ggttatttga ccaggaatgt ttgttaatcc aatgaatgga tggttaatct atgaaactga
     2881 aaaaactccc cgggtttagt ttgggactta ttgctctggc ggtgggtaat gcatatgctg
     2941 gaccgtttat tcagcatgaa gataaggaac at
//
LOCUS       DAZUCE010000361         2934 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZUCE010000361 DAZUCE010000000
VERSION     DAZUCE010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.363"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            39..>2934
                     /locus_tag="ACHY03_004988"
     CDS             39..>2934
                     /locus_tag="ACHY03_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFS3908092.1"
                     /translation="MNLRKKHICIAISLVLNPASISQVSAQADTNKLTVEVDETSLGH
                     DVYGDDWKSIEIGTTKFTAKDESKGINFDHKSPVAEGRNSIAIGTSAEAGGDISTKEG
                     LYTGESIAIGAKTKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGKTYGREKIENN
                     YLASEAKGDGSIALGSRTFAKGELSVSLGSVSSAEGMASNAIGATAEAKGDMATAVGF
                     NALANAGNALAVGSQSSANKNDALAVGSEANAAQNETVALGHGATVTVAKGNVSIGAD
                     SRDKQAQKVTEAKVRTKRGAEVTYSGFAGNNAASVVSVGDAGKERQIVNVGAGAVSEK
                     STDAINGSQLYAIAKTLKDDLDAINPTWKLKDSNGGSKEVGSKNGGTTGNEVTVTGKG
                     DIDVTVDGNGLSIDGTKLAENIKKDAIKIKTGDGKTLTKKLGEEVEFNDAGPIKTKIN
                     NNKLEITAETASFTPDGNGGNGKITVSNGDDDKLVTAKKIADAINQSGWKIKAGSENG
                     GKVEGDNKEELINPGDTVTLKAGKNLSVKQSNGDFTFSVVDTPSFSSVQLNGKGNNGS
                     VMLSSEDNKLTLSGGNGAGQNGVVISGVASGLNNKDLAGLKADDAEMKNAASVADLKT
                     AIDGVSSSMQGAGFALKDSDDKEVKQSLGQAIKVSGDSNITTTAITNGDKGLKISLNN
                     DLTVGDKAPGSIKVNGADGKEAVSLNGQDGTIKLTSSPAAGGKSASATIAVNAGKGGL
                     TDAAGTTKTRLTYAPDGTLPAEELATMNDGLSFEGDDGKIIHKKLNEKLTIKGGAEKN
                     AEVTDGNIRVDNDNGGLLVRMTRNLRDLASAVFGTGGQITTVNDKGITITTPAAGGGQ
                     GNTVSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAANIGDLKNALRDSSAAL
                     VNTGFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRL"
BASE COUNT          905 a          533 c          842 g          654 t
ORIGIN      
        1 actgagtgga agaaaataaa ggagaaaaga gggtatatat gaacctcaga aaaaaacata
       61 tctgtatagc catatcactt gttcttaatc cggcatccat atcacaggtc agtgctcagg
      121 cagatacaaa taagttaact gttgaagttg atgaaaccag ccttggccat gatgtctatg
      181 gcgatgactg gaagagcatt gaaataggta caacgaaatt tacagcaaaa gatgaatcaa
      241 agggaattaa tttcgaccat aaatcaccgg tggctgaagg acgtaactct atcgcgattg
      301 gtaccagtgc agaggctgga ggtgacatta gcacgaagga aggtttgtac acaggcgagt
      361 ctattgcgat aggagcgaaa acgaaagcaa ctaaagaaca gtcaattgca ataggtggcg
      421 ataccaaagc taccggatgg ggggctattg ttattggtgg ggatgattta actcctttgc
      481 atggtaagac ttatggtcgg gaaaaaatag agaataacta tctggcatcg gaagcgaagg
      541 gggatggcag tattgcgctg ggcagcagaa cttttgctaa aggcgaattg tcagtatcgc
      601 ttggttcggt gagttctgct gaggggatgg cctcgaatgc aattggagcc acggcggaag
      661 ctaaagggga tatggcgact gcggttggtt ttaatgcgct ggctaatgcc ggtaatgctc
      721 tggccgttgg tagtcagtcc tctgcaaata aaaacgatgc tcttgctgtt ggttcagagg
      781 caaatgccgc tcagaatgaa acggttgccc tggggcatgg agcaaccgtt actgtcgcaa
      841 aggggaacgt gtccatcggc gctgactcca gggataagca ggcacaaaaa gtaacagagg
      901 ctaaggtcag aactaaaaga ggcgcggaag tcacatatag tggctttgcc ggaaataacg
      961 cagcatcagt agtatctgtt ggtgatgcgg gtaaagagcg tcagattgtt aacgtggggg
     1021 cgggtgctgt atctgagaaa agtacagatg ccattaacgg tagtcagtta tatgcgatag
     1081 cgaagacact gaaagatgac cttgatgcca ttaatccgac gtggaagtta aaagacagta
     1141 acggtggaag taaagaagtc ggcagtaaaa atggcgggac tacaggtaat gaagtgaccg
     1201 tcaccggtaa aggggatatt gatgttactg ttgatggaaa tgggctgagt attgatggca
     1261 caaagcttgc tgaaaacatc aaaaaagatg ccatcaagat taaaacggga gatggtaaaa
     1321 cactaacgaa aaaacttggt gaagaagttg agtttaatga tgccggacca ataaaaacaa
     1381 aaattaataa taacaaactt gagataactg ccgaaacagc cagttttacg cctgatggaa
     1441 atggtggtaa cgggaagatt acagtaagca atggtgatga cgataagctt gttactgcga
     1501 aaaagattgc tgatgccata aaccagtctg gctggaaaat taaagctggc agtgaaaatg
     1561 gtgggaaagt tgagggggat aataaagagg agctaattaa tcccggtgat acagttacac
     1621 tgaaagccgg taaaaatctc agcgtgaaac aaagtaacgg tgattttaca ttctctgttg
     1681 ttgatactcc gtcattcagc agtgttcagc tgaatggaaa aggaaataac ggaagtgtaa
     1741 tgctctcatc cgaagataat aagctgacat tgtcaggcgg taatggtgcc ggacaaaatg
     1801 gtgttgttat cagtggtgtg gcaagtggat tgaataataa ggacctggca ggcctgaaag
     1861 cagacgatgc ggaaatgaaa aatgccgcca gtgttgcaga cctgaaaacg gcaattgatg
     1921 gtgtgtcctc cagtatgcag ggtgcaggtt tcgcactgaa agactctgat gataaggaag
     1981 tgaaacagtc tcttgggcag gcgattaaag tatccgggga cagcaatatc accaccacag
     2041 ccatcaccaa tggggataaa gggttaaaaa tatccctgaa taatgacctg actgtggggg
     2101 ataaggctcc cggcagcatc aaagttaacg gggctgatgg taaggaggct gtgagtctga
     2161 atggtcagga tggcacgatt aaactgacat cctcgcctgc tgcgggcgga aagtctgcgt
     2221 cagcaaccat tgctgttaat gcaggcaaag gtggattaac agatgctgcc ggtaccacga
     2281 agacacgtct gacttatgct ccagatggaa cattaccggc agaagagctg gcgaccatga
     2341 atgacggtct ttcttttgag ggtgatgacg gaaagattat tcataaaaaa ctgaatgaaa
     2401 aactgaccat caaaggtggt gcagagaaga atgctgaagt gactgacggc aatatccggg
     2461 ttgataatga taatggcggg cttctggtga ggatgaccag aaacctccgt gatttggcca
     2521 gcgcggtttt tggtacaggt gggcagataa caacagttaa tgataaaggc atcacaatca
     2581 caacacctgc tgcgggcggt ggtcagggga atacggtttc ccttaccggc tccggcctga
     2641 ataacggtgg aaatcagata accagtgtgg ccagtggtct gaacggcact gcgcttgctg
     2701 acgccggtgg agatgttctg aataatgcgg ccaatatcgg tgacctgaaa aatgctctcc
     2761 gtgacagcag tgcggcactg gtgaataccg gctttggtct gcaggcggat gacggaagca
     2821 aggtgaacac gtctctcggg aagcatatca cggttggtgg tgacgggcag aatatcagca
     2881 cgactgttca gaacgggagc ctgacggtca gactcgctga taacattcgc ctgg
//
LOCUS       DAZUCE010000362         2934 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZUCE010000362 DAZUCE010000000
VERSION     DAZUCE010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.364"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1827)
                     /locus_tag="ACHY03_004989"
     CDS             complement(<1..1827)
                     /locus_tag="ACHY03_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908093.1"
                     /translation="MGLFNYKSYASTQIRELVSGSYNLAIYSNTGKVLGLGSFLASRL
                     FAKHPDRVDYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPCPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAVSNG
                     LSGEDVIVTGYSLGGGLTNIMARLSESLADGFYANSDYIAWASPLVFDGDGKVLNIGF
                     ENDPVFRVLGDESGFWEAVKAMGPGLTTPDYEYDSSPDNIVLYTDLYGSFLWNIIPFS
                     VLALPLGSWSAHFAGSKTDAFERILGSQFYDYMSPDSTVIVDEQSGWTSWYSWVHDKG
                     DDSRERPVFIIGNDGNNLLAGGQGGDYIEGAGGNDKIEPGKGANRVDGGSGYDTLLLS
                     DVMDDWNIYRVSDGTLFFHSERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDH
                     RYLLKWRNKNVDYGKHTEGTNGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGND
                     YLHGTSGNDELYGAEGNDWLYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKS
                     EEDFLFLSKDIVTSTDEFISCLKQTGNNVEAGIAGGRIYFVNT"
     gene            complement(1891..>2934)
                     /locus_tag="ACHY03_004990"
     CDS             complement(1891..>2934)
                     /locus_tag="ACHY03_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908094.1"
                     /translation="FNNAKVHLEKALNTVIQPNDDLYAVYKLAFGKPVGNPEESTTTW
                     REVLNQNGVSGFSVDKNYGGEFRYTYNGLEGSDIISTGYSIGGTARTMISRNDMVINT
                     NGGDDIIAVGMDVGRSYSAGYTDKKYLTSTGDGDDILIVGLGNNNVGIHLKDDASLRI
                     SDRDDVISDGELLLPSSSYDHGSGGIISSTTITMGDGDDTLLALGYEGFGNAIVNTDI
                     DLGAGNDTIHVNGDVKAVSIKGGSGMDILAISHGTLSTKDFSGFEVIDLGDNNGGVNI
                     IASDLVKSGVDAIEGGTLKIIGSSNSGVNLDGDDWKSDGVQSEDGVNYNVYTHEAAPD
                     IKILIDEHITNVM"
BASE COUNT          900 a          755 c          446 g          833 t
ORIGIN      
        1 tgtattaacg aaatatatcc gtcctcctgc aatacctgcc tcaacattat ttccggtttg
       61 tttaagacat gaaataaact catctgtcga tgtaacaatg tcctttgata aaaacagaaa
      121 atcttcttca ctcttatgtt tattaaaatc catgataaat gaattccctt cacaagcagc
      181 atcaaagacg aagacatcat gaccagtacc accatacaga taatcattac ctcccccacc
      241 atataaccag tcgttacctt ctgccccgta caattcatca ttaccagaag tcccatgaag
      301 ataatcattc ccgcgacctc cgtgtaaaag acttactcct tctccccctg cactatgtaa
      361 tatatcattt ccgtcaagac caaaaataat atttccgaac agactatcat ctccgtttgt
      421 accttctgta tgttttccgt aatcaacatt tttattacgc cacttcagaa gatacctgtg
      481 gtctgtaatc tgctcttctt ctatctgata agggcttagt ttactcctcc agtcaccaga
      541 aaaagagata ccttcaatac ctgttgcctc agtcagaccg gctccccgtt ctgaatggaa
      601 aaacagcgtc ccatcactga ccctgtatat attccagtca tccattacat cgctaagaag
      661 cagcgtgtca tacccactgc caccatcaac cctgtttgct cccttacctg gttcaatctt
      721 atcattccct ccagccccct ctatatagtc accaccctgc ccgcctgcca gcagattatt
      781 gccatcatta ccgatgatga atacaggtct ttcacgactg tcatccccct tgtcatgaac
      841 ccatgaatac caggatgtcc agccactctg ctcatccaca atgactgtac tgtccggtga
      901 catgtagtcg taaaactgag accctaaaat tctttcgaag gcgtcagttt ttgaacccgc
      961 aaaatgcgca ctccagcttc ccagcgggag tgcaagcaca gaaaagggga taatattcca
     1021 gaggaatgaa ccataaaggt ctgtgtataa aacaatatta tcaggagaag agtcatattc
     1081 ataatccggg gttgtcaagc ccggccccat tgctttaact gcctcccaga acccggattc
     1141 atctcccagc acacgaaata ccggatcgtt ttcaaaaccg atatttaata ccttcccatc
     1201 cccgtcaaat accagcgggg aagcccaggc tatatagtca gaattagcat aaaaaccatc
     1261 agccagagac tcactgagtc tggccataat gttagtcaac ccgcccccca ggctatagcc
     1321 tgtgacaata acatcttcac cacttaaacc attactgact gcaaagtctc tgatactttg
     1381 aagcaggttt tctgcttcag acagcgcttt tccattattg agctgtgtat aatcaacaat
     1441 atcgcagata tcatttgttc cacagaatga tattgcaatt ttctgtagat tctgttgagc
     1501 atcaaacaac ccaacaactt tcaactgagg accaaatcct gacgcagggt ttcctgtaac
     1561 cggacaggga aagcgataaa aacctcttga atcgacatat tccgaagata accggagttc
     1621 ttccactgta atatcacgcc agccttcatt aagaacatcg gtagaataat caactctatc
     1681 tgggtgcttt gcaaatagcc tggatgccag aaaacttccc aatccaagaa cctttccagt
     1741 gttggaatat atagcaagat tataagagcc agaaacaagc tctctaattt gtgttgacgc
     1801 ataagatttg taattaaata accccataca aacaccttga tgttgaaaaa aactggatag
     1861 aaagagcccc ataggggctc ttttataaga ttacattaca ttcgtaatgt gttcatcgat
     1921 taatatttta atgtctggcg ctgcctcatg tgtatacaca ttatagttaa caccatcttc
     1981 agattgaact ccatcagact tccagtcatc tccatctaag tttacacccg agttagatga
     2041 cccaataatt ttaagtgtgc ctccctctat agcatcaact ccacttttca caaggtcaga
     2101 tgcaattata ttcacccctc cattattatc accaaggtca ataacttcaa acccagagaa
     2161 atctttagtt gaaagggttc catgactaat ggccagaata tccataccag agccaccttt
     2221 aatacttacc gctttcacat caccattaac atgaatagtg tcatttcctg cacctaagtc
     2281 aatatcggta ttaacaatag cattaccaaa tccctcatag cctaaagcaa gaagtgtatc
     2341 atcaccatca cccatagtta tggtggtaga cgatataata ccgccactac catgatcata
     2401 tgaacttgat ggtaataaca gttctccatc actaatcaca tcatctctat cactaatacg
     2461 taaacttgca tcatctttca gatgtatacc aacattatta ttacctaggc cgacaatcag
     2521 aatgtcatca ccatctcccg ttgatgtcaa atattttttg tcagtatagc cagcactata
     2581 gcttctacca acatccatac caacagcaat aatatcatcg ccaccgttag tattaataac
     2641 catgtcatta cgacttatca ttgttctggc agtgccacca atagaatagc ctgttgatat
     2701 gatatcgctg ccctccaatc cattataagt gtatcggaat tctccaccat aattcttatc
     2761 aacactgaag ccagaaacac cattttgatt aagaacttca cgccatgtag tagtgctttc
     2821 ttctggatta cctacaggct taccaaaagc aagtttatat acagcataaa ggtcatcatt
     2881 tggctgaatg acagtattta gtgccttttc caaatggact tttgcattat taaa
//
LOCUS       DAZUCE010000363         2916 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZUCE010000363 DAZUCE010000000
VERSION     DAZUCE010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.365"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            42..1253
                     /gene="bluF"
                     /locus_tag="ACHY03_004991"
     CDS             42..1253
                     /gene="bluF"
                     /locus_tag="ACHY03_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFS3908095.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQCDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDFYTLSPNISDHFAFHPIVDPLSRRIIAFEAIEQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1458..2189
                     /gene="bluR"
                     /locus_tag="ACHY03_004992"
     CDS             1458..2189
                     /gene="bluR"
                     /locus_tag="ACHY03_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFS3908096.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2410..2814
                     /gene="ycgX"
                     /locus_tag="ACHY03_004993"
     CDS             2410..2814
                     /gene="ycgX"
                     /locus_tag="ACHY03_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFS3908097.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFQEYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
BASE COUNT          864 a          611 c          625 g          816 t
ORIGIN      
        1 taccgtctta ctctcacctc tcccattacc ctggacagtt aatgcttact acccttattt
       61 atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat cgaagaaatg gtttcgatag
      121 caaatcgcag gaacatgcag tgtgacgtaa cagggatctt actgtttaat ggttctcatt
      181 ttttccagct tctggaaggt ccggaagaac aggttaaaat gatatatcgg gctatatgcc
      241 aggatcctcg gcactataat attgttgaac tgctgtgcga ttacgcgcct gctcgccgtt
      301 ttggcaaagc gggaatggaa ttatttgatt tgcgcttgca cgagcgagat gacgttttac
      361 aggccgtatt cgacaaaggc acatcaaaat ttcagctaac ttatgatgac agagcgctac
      421 aattttttcg tacttttgtc cttgcaaccg aacaatcaac ctatttcgag atccctgccg
      481 aagattcctg gctttttatc gctgacggat ctgataaaga acttgatttc tatacccttt
      541 caccaaatat aagcgaccac tttgcctttc atccaattgt cgatccctta tcgcggcgga
      601 taatcgcttt tgaagccatt gagcaaaaaa atgaagatag tccatcagct atagcggttg
      661 ggcagcgtaa agacggggaa atctacacag cggatctcaa aagtaaggcg cttgcattca
      721 cgatggcaca cgcacttgag ctcggtgata aaatgatttc aatcaatcta ttacctatga
      781 ccctggttaa cgaacctgac gcagtctctt ttttacttaa tgaaataaag gccaatgctc
      841 tggtgcctga acaaatcatc gttgaattta ctgaaagtga agtcatatct cggtttgatg
      901 aatttgccga agcgattaaa tcgctaaagg ctgctggtat cagtgtagca attgatcatt
      961 ttggcgcagg ttttgctggt ttgttactcc tgtcacgctt ccagcctgac agaattaaaa
     1021 tcagtcagga attgattacc aatgttcata aaagtgggcc acggcaggca attattcagg
     1081 cgatcataaa atgctgtaca tcacttgaaa ttcaagtcag tgctatgggc gtggcaacac
     1141 cagaagagtg gatgtggctt gaatccgcag gaattgagat gtttcaggga gatctgtttg
     1201 ctaaagctaa attgaatggt atcccttcca tagcgtggcc agagaaaaaa taattttcag
     1261 cacattcttt cacatgattt cagtaaatca tcgtgaaaat gaatacgtta cgtttaaatt
     1321 taatccacat taaaaataat gtttcatcca atttagctga aatcttattt atactttgta
     1381 caattttagc gtatattgta caaagtatta ttgggtcgtg tacaggcgac ggagatttgt
     1441 gatcgcaagg aggaattgtg gcttattaca gcattggtga tgttgctgaa cgttgcggga
     1501 ttaatcctgt cactctccgg gcctggcaac gccgctacgg tttgttaaaa ccacagcgca
     1561 gtgaaggcgg acaccgactc tttgatgaag aagacataca acgcatcgaa gagatcaagc
     1621 gttggataag taatggcgtc cctgtaggca aagttaaagc attactggaa accaccagcc
     1681 aggatacgga agatgactgg agccgcctgc aagaagagat gatgtcaatt cttcgtatgg
     1741 ctaatcctgc caaactacgc gcgagaatta tttcactggg tcgagagtac ccagtagatc
     1801 aattgattaa tcatgtttac cttcctgttc gccagcgtct cgtgcttgat cacaacacct
     1861 cccgcattat gagcagtatg tttgacggcg cattaattga atacgcagca acctcgcttt
     1921 tcgaaatgcg ccgtaagccc ggtaaagaag ccattctgat ggcgtggaat gttgaagaga
     1981 gagcacgatt gtggctggaa gcatggcgtt tatcattgtc aggatggcac atttctgttc
     2041 ttgcagatcc cattgaatcg ccgcgcccgg aactgttccc gacgcaaaca ttgattgtct
     2101 ggacaggcat ggcaccaacg agaaggcaaa acgaactttt acaacactgg ggtgagcaag
     2161 gttataaagt catctttcat gccccctaat ttcaccttta tcccgcccct tagacagaat
     2221 gttataatct gaacccacaa cagcatttgt accggagctg atccgttgca aaatgctcac
     2281 cgggagaatt caggtaatcc ctctatcaca ctctgtacat atcacagcca aaggacagac
     2341 cagattgaag tggtaaaacc gatcgtgcac actacgcagt ctcacaacta acaagaggtt
     2401 attatcctga tggatcaggt cgttgttttt caaaagatgt ttgagcaagt gagaaaagag
     2461 caaaacttct cctggtttta ttcagaatta aaacatcacc gtattgcaca ttacatttat
     2521 tatctggcta cggataacat cagaattatt actcacgatg acacggtttt gttattaaga
     2581 ggaaccagaa acctgttaaa agttagtacg accaagaacc ctgctaaaat aaaagaggcc
     2641 gcattgcttc atatttgcgg aaaatctaca tttcaggaat actgttcaac actggcaggc
     2701 gcgggcgttt tccgttgggt tactgatgtt aatcataaca aacgcagtta ctacgccatt
     2761 gataatacgc ttttatatat tgaagatgta gaaaataata aaccattaat ctagctaaag
     2821 ttggatgctt aagaaatgct tcataattca gtaaggcatt agcataatgg aaataaaagt
     2881 gcagagacta tctctatgga tgattaatac tgtctt
//
LOCUS       DAZUCE010000364         2852 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZUCE010000364 DAZUCE010000000
VERSION     DAZUCE010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.366"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            102..734
                     /gene="comR"
                     /locus_tag="ACHY03_004994"
     CDS             102..734
                     /gene="comR"
                     /locus_tag="ACHY03_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFS3908098.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(796..1335)
                     /locus_tag="ACHY03_004995"
     CDS             complement(796..1335)
                     /locus_tag="ACHY03_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFS3908099.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1562..>2852)
                     /gene="ndh"
                     /locus_tag="ACHY03_004996"
     CDS             complement(1562..>2852)
                     /gene="ndh"
                     /locus_tag="ACHY03_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFS3908100.1"
                     /translation="KKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKP
                     LLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKGELL
                     VPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSANL
                     GANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPA
                     LPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDF
                     LKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMAT
                     CAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVY
                     ISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          681 a          777 c          703 g          691 t
ORIGIN      
        1 ttaaatagaa agcaattatt tttatgggat ctggactggt gaacggaggt gattattttt
       61 ataataacct ttcattcaca gtcaccaggt acaacggcat catggcaact gactcaacac
      121 aatgtgtaaa aaaaagccgt ggccgcccaa aagtgttcga cagggatgcc gcgcttgata
      181 aggccatgaa attgttctgg caacacggtt atgaagcgac ttctcttgcg gacctcgtcg
      241 aagcgaccgg agccaaagcg cccacgctat acgcggaatt taccaacaaa gaggggttat
      301 ttcgtgccgt tctcgaccgc tatatcgatc gttttgccgc taagcatgaa gcacagctgt
      361 tttgtgaaga gaaaagcgtg gagtctgcgc tggctgacta ttttgctgcc atcgccaact
      421 gctttaccag caaagacacc ccggcaggct gcttcatgat caacaactgc accaccctct
      481 ccccagattc aggagatatc gccaatacgt tgaaatcacg ccatgcgatg caagagcgca
      541 ctttgcagca gtttttatgt caacgacaag cgcgcgggga aatcccgacc cactgtgacg
      601 tgacgcatct ggcagaattc cttaattgta ttattcaggg gatgtcgatc agcgcacgcg
      661 aaggtgcatc gctggaaaaa ctgatgcaga ttgcccgaac gactttgcgt ttatggccgg
      721 aactgctgaa ataagtcgca aaaaaagccc ctcagcctga gcgaatgagg ggccaaattg
      781 cagagtacag ccttgttata ctttgttatt caaaatcagt tgcccgttgc tatctagcgg
      841 gatctgcgta cccggatcgc ggtccatgcg gattttgccc tgctgatcgc caatcttata
      901 ggtcacatca taaccgagca ttttttctga cttgtcatac accgttttac aacgctgttg
      961 cgtagtcgtg taagtatcgc tttcctggag agagccctgg atctggttac cggcatatcc
     1021 accacccagc gcccccacaa cagtggcgac atctttacca cgaccaccac caaactgatg
     1081 ccctatcacg ccgccagcaa cagccccgag caccgacccg gtaatgcgat tttcatcctg
     1141 caccggtcga cgatgggtca ctgtgacgtt gcgacactcc tgacgcggtg ttttaaccgt
     1201 ttccttgatt ggggttgcag aaacaacctg agcgtattgc gggccccgtt caaacacgtt
     1261 cagactggcc actgccgcta cgcccagcgc agctgcgaca ccaatcccga tacccgccaa
     1321 cattgattta ttcacgggaa cacctccttc tttgcagtta tgccaatcat gcaagcgaag
     1381 gagaaaagca gctatcagac agaagatgaa aagcgcggga atggctggaa atacttaagg
     1441 attcggagga gtctgaagga atgcctgatg cgacgcttaa cgcgtcttat caggcctacc
     1501 gctcgtacca tttgtccagg gcggatgagg cgtttatgcc acatccgcca gtgtacgtcg
     1561 attaatgcaa cttcaaacgc ggacggataa cgcggttaat actccccacc agcatcatta
     1621 atccggtttt aaagtaacca tgcagcgcaa tctgatgcat tcggtatagc gagatatata
     1681 caaagcgcgc aattcgtcct tcaatcatca ttgagccgcg cgtcaggtta cccatcaggc
     1741 taccgacggt ggagaagttc gacagcgata ccagcgaacc atgatcttta tactgataat
     1801 ttttcagcgg cttaccgttc atctgcgcca gtatgttgtt cattgcgcaa gtcgccatct
     1861 ggtgtgcagc ctgagcacgc ggcggaacaa agcccccttc cggacgcggg catgacgcgc
     1921 agtcgccaat agcgtaaatg tctggatcgc gggtggtttg cagcgtcggt tccaccacca
     1981 gctggttgat acggttagtt tcaagaccac cgatatcttt caggaagtct ggcgctttga
     2041 tcccggctgc ccacaccatc agatcagcct caatatattc gccatcttta gtgtgcaggc
     2101 cgccttcatc agcactggtg accatggttt gcgtcagcac gcgaacgcca agtttcgtta
     2161 gctcgttgtg ggccgcagca gagatacgtg gcggtaatgc aggcaaaata cgttctcccg
     2221 cttctaccag cgttacgttc agggcttcgt tggtcaggcc tttgtaaccg tagctgtgca
     2281 gttgcttgac cgcgttgtgc aattcagcgg agagttctac acccgtcgcg ccgccgccga
     2341 caatcgcaat gttcactttg ccatttgcgc ccaggttggc ggagtatttc aggaagagat
     2401 tcagcatctc ctggtgaaag cgacgcgcct ggtgcgggtt atcgaggaaa atgcagttct
     2461 ctttgacacc tggcgtattg aaatcgttag aggtgctacc cagcgccatt accagggtgt
     2521 cataggcgat tttacgttcc ggaaccagca gttcaccttt ctcatcgcgc agttctgcaa
     2581 tagtgattgt tttcgcttca cgatcaatat caatgacgga acccagctgg aactggaaac
     2641 catgattgcg cgcatgggcc agatagctca acgcatcgac gccttcatca agcgagccag
     2701 tcgccacttc gtgcagcagc ggtttccaca ggtggctgtg gttacgatcg accagcgtaa
     2761 ttttggcttt tttcttgcgt cccagcttat gccccagctg tgttgccatt tccagcccac
     2821 cagcaccgcc gccgacaatc acaatttttt tc
//
LOCUS       DAZUCE010000365         2827 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZUCE010000365 DAZUCE010000000
VERSION     DAZUCE010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.367"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..243
                     /locus_tag="ACHY03_004997"
     CDS             <1..243
                     /locus_tag="ACHY03_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFS3908101.1"
                     /translation="DITHLYRAFMSVEKLTLSGRQVNVLMMLLYGSNGTEGARHLGMS
                     EKTFSTHKQNLIKMLRVHNEVELLYKGMLLDRKGRL"
     gene            318..506
                     /locus_tag="ACHY03_004998"
     CDS             318..506
                     /locus_tag="ACHY03_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFS3908102.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            602..1498
                     /gene="mscS"
                     /locus_tag="ACHY03_004999"
     CDS             602..1498
                     /gene="mscS"
                     /locus_tag="ACHY03_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFS3908103.1"
                     /translation="MEKLDVMDASGEAVSWIVRNQDILLGYAVNLVAAVVIIIIGGIA
                     ARMISGVLNNLMLGRNIDATVAQFLSVLIRHAIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDG
                     KIVVIPNGKIIAGEIVNFSREPERRNEFIISVSYDADIDRVKQVLTDIVMSEERVLKN
                     REITVRLNELGESSMNFVVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIH
                     LVRSGKQAESVTHNSRSVSSRQ"
     gene            1624..>2827
                     /locus_tag="ACHY03_005000"
     CDS             1624..>2827
                     /locus_tag="ACHY03_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFS3908104.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWSDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRK"
BASE COUNT          701 a          528 c          781 g          817 t
ORIGIN      
        1 gatatcactc atctgtaccg cgcattcatg tcggtggaga agctgaccct gtcaggccgg
       61 caggtaaatg tactgatgat gctgctttat ggcagtaacg gaacagaggg ggcacgacat
      121 cttggcatgt cagagaaaac attctccact cataagcaga atctgataaa gatgttacgg
      181 gtacataatg aagttgagtt gttgtacaag gggatgcttc tggacagaaa agggaggttg
      241 tgaagcagga cagcttattt ccggtaatgt taaacgcagc ctctgattcg ttaatttaat
      301 aaacataagg agtttttatg cgtctgttga ttgcttttct tcttccctgg ctgacttttt
      361 tcaccattgg ccggcctctg acggggattt tttgtctggt gttacagata acgctgctgg
      421 gctggatacc ggcaacaata tgggctgtgt atgcgctttc gcagtataag acggaacaga
      481 aaatccgcga agccacgggg aaatgatgac tgcagaagag caggttgtca tggctgtgta
      541 tatgatgcgg gataaaggcc cggagattcc tgaaaataaa atggttcgtg gaatataaaa
      601 tatggaaaaa cttgatgtga tggacgcttc tggcgaggcg gtttcatgga ttgtccgtaa
      661 tcaggatata ttactcggtt acgccgtgaa tcttgtggca gcagtggtta ttattattat
      721 cgggggtatc gctgcacgga tgatttctgg tgtgctgaat aatctgatgc tgggcaggaa
      781 tattgatgcc acagtggcgc agttcctttc cgtgctgata cgtcatgcca ttatagcctt
      841 tacgctgatt gctgccctgg gacgtgtggg ggttcagaca gcttccgtaa ttgcagtcct
      901 gggggctgca ggtcttgctg ttggacttgc cctgcagggg tcattgtcaa accttgctgc
      961 tggtattctt ctcgtgatgt tccgtccttt cagggctggt gaatatgctg acctgggtgg
     1021 tgttgccggg actgtgcaga atgttcatat attctcaacc acgatgcgta cactggatgg
     1081 taaaattgtt gtcattccga acgggaagat tattgccgga gagattgtta atttctccag
     1141 ggagcctgaa cgacgaaatg agtttatcat cagtgtttca tatgatgcag atattgaccg
     1201 tgtgaaacaa gtcctgacag atattgtgat gtcagaagag agggttctga agaacaggga
     1261 aatcactgtt cgcctgaatg agcttgggga gtcatccatg aattttgtag tacgggtatg
     1321 gagcaggcgt gatgaccttc agtctgtata ctgggatgta ctggagagaa ttaagcggga
     1381 gtttgattca gaagggataa gttttccata tccacaaatg gatatccatc ttgttcgctc
     1441 cgggaagcag gcagagtcgg taacccataa cagtcgctca gtcagttcca gacagtaaaa
     1501 tgaaaagcgc attttatttg tgaaagatga acagcctgaa tctgtcaggc tgttttcatt
     1561 gtgtttttat ggtcattttt ggtatatgta ctgtatatga tgtcatctgt ataaggatat
     1621 aaaatgccaa agaaaataat gacagggatt gatgtttttg acgctgccat ggagcgtatt
     1681 gaatgggtgt ttgatacgtt ttcatcagtt tgtctttctt tttccggcgg aaaagactcc
     1741 actgtcctgt ttcacctgat ggcgaatatt gcccgccgaa aaaaacgccg tttttctgta
     1801 ctgtttgttg actgggaggc tcagtatcag tgtaccattg agcatgtcac caggttgagg
     1861 gaaatgtacg atgatatcac agatactttt ttctgggtgg cgctgcctct gacgaccgtt
     1921 aatggagtgt ctcaggtaca gcctgagtgg gtatgttggg aggacggggc tgaatgggtt
     1981 cgccagccac cagaagatgc cataaccaga gcgtcttttt ttccttttta tcagaatgcg
     2041 atgacatttg aagagtttat tccggcattt aatcattggt ttgcccggga aaaagggatg
     2101 gttgctgcga ttcttactgg tgttcgcgct gatgagtcac tgaatcgttt tatgggactg
     2161 gtatccggac gtaagctccg ttatgcggag gataaaccct ggaccacggc atcggctgag
     2221 ggattttact acaccatgta tcctttgtac gactggaaaa caagagatgt atggatttac
     2281 catgcgagaa cacgtgctgc ctataactct ctttatgacc tgatgtacag ggctggagtt
     2341 cctcttcgca atatgcgggt gtgtgagcct tttggaccgg agcaacgtaa ggggttgtgg
     2401 ctttatcatg tactggagcc ggatacatgg ggcagaatgt gtgctcgcgt atcaggtgct
     2461 gtagggggag cgctctatgc aaatgaaagt ggtgcttatt ttgctttgag gaaacagata
     2521 acaaaaccag ttcaccatac atggcagagt tatgcaatgt ttctgctgga gtcaatgcct
     2581 gagaaaacgg cggagcatta caagaataag attgctgttt atcttcggtg gtggcagtct
     2641 catggatggt cggatggcat ccctgatgag caggataatg accagggatg taaagatatt
     2701 ccctcctggc gtcgcatctg taagacgtta ctgaaaaatg atttctggtg tcggatgctg
     2761 gcgtttagtc cgaataagcc ccgacattat gagcgctacc tggccagaat gcgtgaaaaa
     2821 aggaaag
//
LOCUS       DAZUCE010000366         2796 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZUCE010000366 DAZUCE010000000
VERSION     DAZUCE010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.368"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            116..613
                     /locus_tag="ACHY03_005001"
     CDS             116..613
                     /locus_tag="ACHY03_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HFS3908105.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLCIYDFQNYNYLKGYAAVCQLKKDYQKAFDMYHICLML
                     SPDNDFSLVYYMGQCQMGLKNTKMATELFNTVVTYSQNEKVKEMATAYLELLTANTEE
                     EVQTE"
     gene            623..805
                     /locus_tag="ACHY03_005002"
     CDS             623..805
                     /locus_tag="ACHY03_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908106.1"
                     /translation="MNINGKNASFTPEELTRKYHEEAPVDLKNNHGDKINEKNNNKHI
                     TYFPVDAILDGNKDLP"
     gene            861..2642
                     /locus_tag="ACHY03_005003"
     CDS             861..2642
                     /locus_tag="ACHY03_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopB/SseC family type III secretion system
                     translocon subunit"
                     /protein_id="HFS3908107.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSSKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPAMQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     TTRLHNNLDSTLLRHEMAHNKFRELSDAYASSLDSAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVLTQKNAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CDSIKNQSEVMKEINHVRENKLNETARKYTTTTKVLKIVNECVTVVTIAMSAILIVVG
                     LLAAVPSGGSSIAGALALIGGIAGAVVLGVDITCQIALGTTATGWILGKVVEGLSAAI
                     KTVDPTLLAITALLDVIGVDQNTIDLVKNIYASAAASIIMATVMIGAAVICSVAIGAV
                     VSALSKTAAEEVTKEITNTIKSTIESIINSVSKNIIKVLDSVCSVLQASAVVLKLIAK
                     ISNGLEKIGLLICAIATSTMNCFVAGNSADMAILQQDMSNLSKTREQMLSVLQRVDKT
                     VEQEVSQMVRVLQHRTEALKFASHSIV"
BASE COUNT          986 a          526 c          567 g          717 t
ORIGIN      
        1 taatgcagaa aaggaagccc aacggatggg cataaaaatg agcgaatttt gatatcacca
       61 ccctctaaaa aatggtgaag tctgaacaaa gaatgttcga aaggagagag tagagatgga
      121 aagcgcaaat ctggatttag aagaaaataa agaaattgcc agtaagttcg aacgtgcact
      181 aggtatggga gcaacgcttg cagaactgca tggcattact ccggataccc ttgaaggcgt
      241 ttatgcttac gcttataact tttatgaaaa aggaagactt gacgaagctg aactcttttt
      301 taaatttctc tgtatttatg atttccaaaa ttataactac ttaaaaggat atgccgccgt
      361 ttgtcagttg aagaaagatt atcaaaaggc attcgacatg tatcatatct gtttaatgct
      421 atctcccgat aacgatttct ctttggttta ttatatgggc caatgccaga tgggtttaaa
      481 aaacactaaa atggccacgg agttatttaa tacagtcgta acctacagcc aaaacgaaaa
      541 agttaaagag atggctactg cttatcttga acttttgaca gccaataccg aagaggaagt
      601 tcaaacagag tgagtcggca aaatgaacat aaacggaaaa aacgcttctt ttacgcctga
      661 agagttaacc aggaaatatc atgaggaagc cccggttgat ttaaaaaata accatggcga
      721 taaaataaat gagaaaaaca acaataaaca cattacttac tttcccgttg atgccatttt
      781 agatgggaat aaagatctgc catgagttaa ataaaaaaga attaattaca cccgatgata
      841 gcttaaaaag gaggtgaatt atgtcggcac cgatcacggg acaaacgatc acgtttgaac
      901 aaatcagcga aacattgcgt acacagtata gcgatgcgga aaaaagactg caggacagca
      961 gcaaaacgca agttgatccc atgcggctta ataaaaaccc gaaatccctt gataacgata
     1021 ttcgcgctcg tcttgaaaat aaacctatgc ttgctccgcc tgcaatgcag gtttcagatt
     1081 cagacaacgc cactacagca aagacgaatg atgcccgcct gacaatgatt ttgggtaatt
     1141 taacaggcat tgccgatcag gatattacaa cacgattgca taataacctt gacagcaccc
     1201 tgcttcgaca cgaaatggca cataataagt ttcgtgaatt aagcgacgcc tatgcatcct
     1261 cattagattc tgcgcaaaaa gctgacgata tcatgcatca ggccaacaat aattacaatg
     1321 cggtcgataa aaaggtgcaa tcgctggaga aaaaagtcaa tacattgaat caagagctgt
     1381 cgcaactgca acctggcgat ccgcaatata ataaagtact gacgcaaaaa aatgcggctg
     1441 agaaaacact cacgctgtca ttacaaaaaa aatcgttagc tgagaaatcg ttaaatacag
     1501 ccattatgga tgctgatgct gcgatcggcc aaagtatgga aatttttgac gaaattcaac
     1561 agcaagaaca gattaataac tttactacca atatttgcct gacccaggaa aaccagaaaa
     1621 acagaaacgc gacagccaca tttatccttt tgattacctc agtaatggaa gttattggtg
     1681 ataccaattg cgactctatt aaaaatcaat ctgaggtaat gaaagagatt aaccatgtca
     1741 gggaaaataa actcaatgaa acagcgcgta aatatactac cacgactaaa gttttaaaaa
     1801 tcgttaatga gtgtgtaact gtagttacca ttgccatgag cgcaatccta attgttgttg
     1861 gtcttcttgc tgcagttcct agtggtggtt caagtattgc gggtgcactg gcgcttattg
     1921 gtggaatagc gggcgcagtg gtattaggtg ttgatatcac ttgtcaaatc gctctgggta
     1981 ccacggctac cggctggatt ttagggaaag ttgttgaagg tctttcggct gctattaaaa
     2041 cagttgatcc cactctgctc gcaatcactg cgcttctgga cgttattggt gtcgatcaaa
     2101 atacgattga tctggttaaa aatatttacg caagtgcagc cgcttctatt atcatggcga
     2161 cagtaatgat cggtgcagct gtaatatgtt ctgtagcaat aggggccgtt gtttctgcat
     2221 tatcaaaaac agccgccgaa gaagtcacaa aagaaataac aaacactatc aaatcaacca
     2281 ttgaatcaat tattaattca gtttcaaaaa atattataaa ggtattagac agcgtatgca
     2341 gcgtgctaca agcatcagcc gtagtgttga agttgattgc caaaataagt aatggcctgg
     2401 agaaaatagg cttactcatc tgtgcaatag caacatcgac gatgaattgt tttgttgctg
     2461 gaaactctgc cgacatggca attttacaac aggacatgag taatctatca aaaacgcgtg
     2521 aacaaatgct ttcagtattg caaagggtgg ataaaaccgt cgaacaagag gtaagtcaga
     2581 tggtaagagt attacaacac cgaactgaag ccttaaaatt tgcttcccat tctatcgtat
     2641 aagagggttt taacatggcc acctatgaaa ttaaaaatgc gaccagcata cataatatta
     2701 acaatctcga taaaacaacc tttgatagag attatggtaa agaaaacaac ataccaaatg
     2761 ataataaaat tgtgcaatac tataacctta aagata
//
LOCUS       DAZUCE010000367         2765 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZUCE010000367 DAZUCE010000000
VERSION     DAZUCE010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.369"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..275
                     /locus_tag="ACHY03_005004"
     CDS             <1..275
                     /locus_tag="ACHY03_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS3908108.1"
                     /translation="EATGLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHI
                     PEQLSKRERYTDKWQRRTHSLWRHRAWHVSAFTGICNDLAAASTFV"
     gene            173..820
                     /locus_tag="ACHY03_005005"
     CDS             173..820
                     /locus_tag="ACHY03_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS3908109.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGENELSARLNEPLKNGAVIHIVPRLAGAKSGGV
                     FQAVLGAALIAVAWWNPAGWLGAAALSGMYAAGASMILGGVAQMLAPKASTATTTSTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            880..>2765
                     /locus_tag="ACHY03_005006"
     CDS             880..>2765
                     /locus_tag="ACHY03_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFS3908110.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQCCDQSVPDGFGGTEPRITCNAWLTTQRK
                     VWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVT"
BASE COUNT          592 a          712 c          916 g          545 t
ORIGIN      
        1 tggaggcaac ggggctgtat caggtgccgt tgtcagcggc gcagccgggc gatgtgctgc
       61 tgtgctgttt tggttcatcg gtgccgaatc atgccgctat ttactgtggt gacggcgagc
      121 tgctgcacca tattcctgaa caactgagca aacgagagag gtataccgac aaatggcagc
      181 gacgcacaca ctccctctgg cgtcaccggg catggcacgt atctgccttt acggggattt
      241 gcaacgattt ggccgccgca tcgaccttcg tgtgaaaacg ggggccgaag ccatccgggc
      301 gctggccatg cagatcccgg cgtttcgtca gaaactgagc gatggctggt atcaggtgcg
      361 cattgccggg cgtgatgcag gcgaaaacga attatcagcc cgtcttaatg agccgctgaa
      421 aaatggtgcc gtgatccaca tcgttccgcg tcttgcggga gcaaaaagtg gcggtgtgtt
      481 tcaggcggtg ctgggtgcgg cgctgattgc ggtggcgtgg tggaaccctg caggctggct
      541 gggagctgcg gctttatcgg gcatgtatgc tgcgggggcc agtatgatcc ttggcggtgt
      601 ggcgcagatg ctggcaccga aagccagtac cgcaacaacg accagcacgg ataacggtaa
      661 gcagaacacg tatttctcgt cactggataa catggttgcc cagggcaatg ttctgcctgt
      721 tctgtacggt gaaatgcgcg tggggtcgcg ggtggtctct caggagatca gcacggcaga
      781 cgaaggggat ggtggtcagg ttgtggtgat tggtcgctga tgaaaaacgt ttatgtgaaa
      841 ccgcctccgg gcggttttgt cgtttatgga gcgtgaggaa tgggtaaagg cagcagtaag
      901 gggcatactc cgcgcgaagc gaaggacaac ctgaagtcca cgcagttgct gagtgtgatc
      961 gatgccatca gcgaagggcc ggttgaaggt ccggtggatg gattaaaaag cgtgctgctg
     1021 aacagtacgc cggtgctgga cagtgagggg aataccaaca tcgccggtgt cacggtggtg
     1081 ttccgtgccg gtgagcagga gcagacaccg ccggaggggt ttgaatcctc cggctccgag
     1141 acggtgctgg gtacggaagt gaaatatgac acgccgatca cccgcaccat tacgtctgca
     1201 aacatcgacc gtctgcgctt taccttcggt gtgcaggcac tggtggaaac cacctcaaag
     1261 ggggaccgga atccgtcgga agttcgcctg ctggttcaga tacagcgtaa tggtggctgg
     1321 gtgacggaaa aagacatcac cattaagggc aaaaccacct cgcagtatct ggcctcggtg
     1381 gtggtggata acctgccgcc gcgcccgttt aatatccgga tgcgcaggat gacgccggac
     1441 agcaccacag accagctgca gaacaaaacg ctctggtcgt catacaccga aatcatcgat
     1501 gtgaaacagt gctacccgaa cacggcactg gtcggcgtgc aggtggactc ggagcagttc
     1561 ggcagccagc aggtgagccg taattatcat cttcgcggac gcattctgca ggtgccgtcg
     1621 aactataacc cgcagacgcg gcaatacagc ggtatctggg acggaacgtt taagccagca
     1681 tacagcaaca acatggcctg gtgtctgtgg gatatgctga cccacccgcg ctacggcatg
     1741 ggtaaacgtc ttggtgcggc agatgtggat aaatgggcgc tgtatgtcat cggccagtgt
     1801 tgcgaccagt cggtgccgga cggttttggc ggcacggagc cgcgcatcac ctgtaatgcc
     1861 tggctgacca cacagcgtaa ggtgtgggat gttctcagtg atttctgctc ggcgatgcgc
     1921 tgtatgccgg tatggaacgg gcagacgctg acgttcgtgc aggaccgacc atcagataag
     1981 gtgtggacct ataaccgcag taatgtggtg atgccggatg atggcgcgcc gttccgctac
     2041 agcttcagcg ccctgaagga ccgccataat gccgttgagg tgaactggat tgacccggac
     2101 aacggctggg agacggcgac agagcttgtg gaggatacgc aggccattgc ccgttacggt
     2161 cgtaacgtca cgaagatgga tgcctttggc tgtaccagcc gggggcaggc acaccgcgcc
     2221 gggctgtggc tgattaaaac ggagctgctg gaaacgcaga ccgtggactt cagcgtgggt
     2281 gctgaagggc ttcgccatgt accgggcgat gtcattgaaa tctgcgatga tgactatgcc
     2341 ggtatcagca ccggcgggcg cgtgctggcg gtgaacagcc agacccggac gctgacgctc
     2401 gaccgtgaaa tcacgctgcc atcctccggt accacgctga taagcctggt tgacggaagt
     2461 ggcaatccgg tcagcgtgga ggtccagtcc gtcaccgacg gcgtgaaggt gaaagtgagc
     2521 cgtgttcctg acggcgttgc cggatacagc gtatgggggc tgaagctgcc gacgctgcgc
     2581 cagcgcctgt tccgctgcgt gagtatccgt gagaacgatg acggcacgta tgccatcacc
     2641 gccgtgcagc atgtaccgga aaaagaggcc atcgtggata acggggcgca ctttgacggc
     2701 gaccagagcg gcacggtgaa tggtgtcacg ccgccagcag tgcagcatct gaccgcagaa
     2761 gtcac
//
LOCUS       DAZUCE010000368         2760 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZUCE010000368 DAZUCE010000000
VERSION     DAZUCE010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.370"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1886)
                     /locus_tag="ACHY03_005007"
     CDS             complement(<1..1886)
                     /locus_tag="ACHY03_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFS3908111.1"
                     /translation="MGKGSSKGHTPREAKDNLKTTQLLSVIDAISEGPIEGPVEGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSLTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPRL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEVIVDNGAHFDGEQSGTVNGVTPPAVQHL
                     TAEVT"
     gene            complement(1947..2549)
                     /locus_tag="ACHY03_005008"
     CDS             complement(1947..2549)
                     /locus_tag="ACHY03_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS3908112.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPSFRQKLNE
                     GWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(2486..>2760)
                     /locus_tag="ACHY03_005009"
     CDS             complement(2486..>2760)
                     /locus_tag="ACHY03_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS3908113.1"
                     /translation="EATGLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHI
                     PEQLSKRERYTDKWQRRTHSLWRHRAWHASAFTGIYNDLAAASTFV"
BASE COUNT          555 a          905 c          698 g          602 t
ORIGIN      
        1 gtgacttctg cggtcaggtg ctgcaccgct ggcggcgtga caccgttcac cgtgccactc
       61 tgttcgccgt caaagtgcgc cccgttatcc acgatgacct ctttttccgg cacatgctgc
      121 acggcggtga tggcatacgt gccgtcgtcg ttctcacgga tactcacgca gcggaacagt
      181 cgctggcgca gcctcggcag cttcagcccc catacgctgt attcagcaac accgtcagga
      241 acacggctca ctttcacctt cacgccgtcg gtgacggact gaacctccac gctgaccgga
      301 ttgccacttc cgtcaaccag gcttatcagc gtggtaccgg aggatggcag cgtgatttca
      361 cggtcaagcg tcagcgtccg ggtcaggctg ttcaccgcca gcacacgacc accggtgctg
      421 ataccggcat agtcatcatc acagatttca ataacatcgc ccggtacatg gcgaagccct
      481 tctgcgccga cgctgaaatc cacggtctgc gtttccagca gctccgtttt aatcagccac
      541 agcccggctc ggtgtgcctg cccccggctg gtacagccaa aggcatccat cttcgtgaca
      601 ttacgaccgt aacgggcaat ggcctgcgtg tcctccacaa gctctgtcgc cgtctcccag
      661 ccgttatccg ggtcaatcca gttcacctca acggcattat ggcggtcctt cagggcgctg
      721 aagctgtagc ggaacggcgc gccatcatcc ggcatcacca cattactgcg gttataggtc
      781 cacaccttat ccgacggtcg gtcctgcacg aacgtcagcg tctgcccgtt ccataccggc
      841 atacagcgca tcgccgagca gaaatcactg agcacatccc acgccttgcg ctgtgtggtc
      901 agccaggcat tacaggtgat gcgcggctcc gtgctgccaa agccgtccgg caccgactgg
      961 tcgcagtact ggccgatgac atacagcgcc catttatcca catctgccgc accaagacgt
     1021 ttccccatgc cgtagcgcgg gtgggtcagc atatcccaca gacaccaggc catgttgttg
     1081 ctgtatgccg gtttaaacgt tccgtcccag ataccgctgt attgccgcgt ctgcgggtta
     1141 taattcgacg gcacctgcag aatacgcccg cgcagatgat aattacggct cacctgctgg
     1201 ctgccgaact gctccgaatc cacctgtacg ccgaccagtg ccgtgttcgg gtagcactgt
     1261 ttcacatcga tgatttcggt gtatgacgac cagagcgttt tgttctgcag ctggtctgtg
     1321 gtactgtccg gcgtcattct gcgcatccgg atattaaacg ggcgcggcgg caggttatcc
     1381 accaccaccg aggccagata ctgcgaggtg gttttgccct taatggtgat gtctttttcc
     1441 gtcacccagc caccattacg ctgtatctga accagcaggc gaacttccga cggattccgg
     1501 tccccctttg aggtggtttc caccagtgcc tgcacaccga aggtaaagcg cagtcggtcg
     1561 atgtttgccg acgtgatggc gcgggtgatc ggcgtgtcgt atttcacttc cgtacccaac
     1621 accgtctcgg agccggagga ttcaaatccc tccggcggag tctgctcctg ctcaccggcc
     1681 cggaacacca ccgtgacgcc ggagatattg gtattcccct cactgtccag caccggcgta
     1741 ctgttcagca gcacgctttt taatccctcc accggacctt caatcggccc ttcgctgatg
     1801 gcatcgatca cactcagcaa ctgcgtggtc ttcaggttgt ccttcgcttc gcgcggagta
     1861 tgccccttac tgcttccttt acccattcct cacgctccat aaacgacaaa accgcccgca
     1921 ggcggtttca cataaaacat tttgcatcag cgaccaatca ccacaacctg accaccatcc
     1981 ccttcatctg ctgtgctgat ctcctgagat accacccgcg accccacacg catttcaccg
     2041 tacagaacgg gcaggacatt gccctgggca accatattat ccagtgaaga aaaataggtg
     2101 ttctgtttgc cgttatccgt tgtctgtgtg cggggagttc tggctttcgg tgccagcatc
     2161 tgcgccacac caccgagcac catactggca ccgagagaaa acagcagatt actcgccata
     2221 attcctaccc ccggcatcca tatagcaacc gccataacag ccgcccccag cacagcctga
     2281 aacacaccgc cacttttggc tcctgccaga cgcggcacga tatggatcac agcaccattt
     2341 gccagcggtt cattaagacg ggctgataat tcattttcgc ctgcatcacg cccggcaatg
     2401 cgcacctgat accagccctc attcagtttc tggcgaaacg acggaagctg cgtggccagc
     2461 gcccggatgg cttcggcccc cgttttcaca cgaaggtcga tgcggcggcc aaatcgttgt
     2521 aaatccccgt aaaggcagat gcgtgccatg cccggtgacg ccagagggag tgtgtgcgtc
     2581 gctgccattt gtcggtgtac ctctctcgtt tgctcagttg ttcaggaata tggtgcaaca
     2641 gctcgccgtc gccgcagtaa atggcggcat gattcggcac cgatgaacca aaacagcaca
     2701 gcagcacatc gcccggctgc gccgctgaca acggcacctg atacagccct gtggcctcca
//
LOCUS       DAZUCE010000369         2742 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZUCE010000369 DAZUCE010000000
VERSION     DAZUCE010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.371"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..2424)
                     /locus_tag="ACHY03_005010"
     CDS             complement(<1..2424)
                     /locus_tag="ACHY03_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFS3908114.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMRGEKVSEYRF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLPKVLLTKLGVKTGNLNTEDNC
                     ILLDEAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSHTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGKWQSNTLYLERGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYTIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGSVRSFLVPYSSVPNMLQPG
                     ISNFDFIAGRSQIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDTRHEGTSYQVAYNKYLLQTATHFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNNWQHISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NNKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTINFQHVID"
     gene            complement(2437..>2742)
                     /locus_tag="ACHY03_005011"
     CDS             complement(2437..>2742)
                     /locus_tag="ACHY03_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFS3908115.1"
                     /translation="ALQNRIKLLWRPSGIAPVDKKSLSQLNIKKKNNAISINNETANW
                     ITVTTIKAQNVKVNNESILLPPFSNNDITLKNNHASEYELTVVDDYGNNIHSKIAAR"
BASE COUNT          760 a          550 c          555 g          877 t
ORIGIN      
        1 atctataacg tgttgaaaat taatagtaca gaataaattc tgttcatcat tgagagcaac
       61 ttttatgcca gttggaattt catctacgcg aataaaaagg cgactaccct ggccaacgac
      121 tccaatattg ttttcctgga gatccagtac atcatagcca aaagttaatg gcgaaccatc
      181 gggacgcagt gcctgtatat accagggctt acgttggtca gttgtaaact gcacataaga
      241 aactgctcca cggtaaggcg caataatttg acgattgccc tgcaattctg tttcactttc
      301 actgtttgct gtatcaagta ccagatggtt ttcacgatga ggtatcatca ggtcgtaaac
      361 aacctggcct ttgttgtttg ttcggttgta tttttgtcca ttaatatgcg cgccttctaa
      421 cccaggcgca tccagaattg caaatgtatc ggatagctgg ttagtgatat taataccacc
      481 gggccagaca actaatccac tactaatact tccaccactt tgccaggcgt ttttagaatg
      541 gctatagctg ccatctattg tggcgattgg agttctccag cttaaattcg taccaagcga
      601 ggtatcatta ttttgttgtt gctgattaac atatatacca taatttaact gatcatgttc
      661 accggctatg ccagtaattc cagtattgtt ggaggaatag ccatctttgg aaaatgaggt
      721 agaattcgat aagttaattt ggtgacgtgt tttggcaata tcatcacccc agtagaaagg
      781 aatggagata aacagattaa aacgctcctc ttctttatcg ttttcatcat aggattggct
      841 ggcagagaaa gtataactaa tgtgttgcca gttattggag taactgaatt ggtaatcttt
      901 agcatttcca cttcgccccc agtaattccg ccaaagtgca cttaatgata catttcccag
      961 attattggat aaaggttgca ttatattggc agaaaggcta ttttttctgc cgatatcata
     1021 aaaatcgtca tagtcactct gatgatttat tttatcattt tcgtaaagat ggtcgctgaa
     1081 tgtcctgtaa tcctgcgaag cataacgcca ggcggccacg ctaaagtgag tggccgtttg
     1141 caatagatat ttattatagg cgacctgata gctcgtacct tcgtgtctgg tatcattatt
     1201 taacttacta ctcgatcggg tagcgtcaaa agagatggca cccagcggtg tattccaacc
     1261 atttcccaga gttatagcat tatagttatc ggataaaatc gtaccgccat aaagtgtcaa
     1321 aagattgttg aggccatata tataatttgc ctcgagaaag tcctcctgat tcttgacacc
     1381 atatatctga ctacgtccgg ctataaaatc gaaatttgaa ataccaggtt gcagcatatt
     1441 gggcacggaa gagtagggca ccaaaaagga acgaacacta ccatctgctt ccttaatgct
     1501 gacatcgaga tcagagccgc tgcccgaaag ttgcaaatct gcgatagtaa atggtcctgg
     1561 gggaacctct ttttggtaaa tggtatagcc attttgcgaa accgttatca aggcgttact
     1621 ttgcgcaaca ccttgtacta aaggtgtaaa gctttgcata gaatcaggta gcatttgcat
     1681 atcgcgaaat aagcgtattc cgctaaatcg cagagagtcg aatatgagtg aggatgtgta
     1741 attttctcct atctgaactg tgcttaatat ctgcgaccag ccacgttcca ggtaaagagt
     1801 attactttgc catttgcctt tcatatcatc aggtttgcta taactggcat cagagtgtaa
     1861 ctgccaacta aataaattta atccgctgtt aaaccggcca taactggctg tatgactgtt
     1921 attattgctg tcataagagc gatactgact taaattatag gaggtataaa aagcatcaat
     1981 gccgcgatcc caactttctg ggggaacgta gcctctttca agttcattga tatacgcctg
     2041 tggtactgtt aaatttagtc ggtgttcact gatatcccac tgatattgtc caccatgaac
     2101 cgcttcatcc agtaaaatac aattatcttc tgtattgagg ttaccggtct ttacccccag
     2161 tttggttagt agcaccttag gcagacatgg tttgactggt gactctggga tcgtgatatc
     2221 ttgttttcca cgccactggt tatttacata aaaatcaagt tcgtagtttc ctggaagcgg
     2281 ttgtttatta tcgaaacgat attcggaaac tttttctcct ctcatgccgc caatcataaa
     2341 gtgcgtatca aaagtttctt cagcataacc cggaaaagaa tacaaaaaaa taatagcagc
     2401 gacccatcgt gtcgtcctta acatgacttt ttcctgttat ctcgcggcga ttttactatg
     2461 gatgttattc ccgtaatcat caactaccgt tagttcatat tctgatgcgt ggttattttt
     2521 taatgtaatg tcgttatttg aaaatggagg cagtaagata ctttcattat taactttcac
     2581 gttttgtgct ttgatagttg taaccgttat ccagttggct gtttcgttgt ttattgagat
     2641 ggcgttattt ttctttttga tatttaattg agaaaggctt tttttatcta ccggagctat
     2701 tccgcttgga cgccaaagta gctttatacg gttttgcaat gc
//
LOCUS       DAZUCE010000370         2695 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZUCE010000370 DAZUCE010000000
VERSION     DAZUCE010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.372"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            172..1338
                     /gene="nhaA"
                     /locus_tag="ACHY03_005012"
     CDS             172..1338
                     /gene="nhaA"
                     /locus_tag="ACHY03_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFS3908116.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1404..2303
                     /gene="nhaR"
                     /locus_tag="ACHY03_005013"
     CDS             1404..2303
                     /gene="nhaR"
                     /locus_tag="ACHY03_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFS3908117.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2341..>2695)
                     /locus_tag="ACHY03_005014"
     CDS             complement(2341..>2695)
                     /locus_tag="ACHY03_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFS3908118.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMNRVKIVVMYN"
BASE COUNT          628 a          597 c          692 g          778 t
ORIGIN      
        1 gtcacccgtc agcgcttaca gccgtcaaaa acgcatctca ccgctgatgg cacaaattct
       61 tctatagatc gtaaaaaacg aattattcct acactataat ctgattttaa cgatgattcg
      121 tgcggggtaa aatagtaaaa acgatctatt cacctgaaag agaaataaaa agtgaaacat
      181 ctgcatcgat tctttagcag tgatgcctcg ggaggcatta ttcttatcat tgccgctatc
      241 ctggcgatga tgatggccaa cagcggcgca accagtggat ggtatcacga ctttctggag
      301 acgccggttc agctccgggt tggttcactc gaaatcaaca aaaacatgct gttatggata
      361 aatgacgcgc tgatggcggt atttttcctg ttagtcggtc tggaagttaa acgtgaactg
      421 atgcaaggat cgctagccag cttacggcag gccgcatttc cagttatcgc cgctattggt
      481 gggatgattg tgccggcatt actctatctg gcctttaact atgccgatcc gattacccgc
      541 gaagggtggg cgatcccggc ggctactgac attgcctttg cacttggtgt gctggcgcta
      601 ttgggaagtc gtgttccgtt agcgctgaag atctttttga tggctctggc tattatcgac
      661 gatcttgggg ccatcattat catcgcattg ttctacacta atgacttatc gatggcctct
      721 cttggcgtcg cggctgtagc aattgcggta ctcgcggtat tgaatctgtg tggtgtacgc
      781 cgcacgggcg tctatattct ggttggcgta gtgttgtgga ctgcagtgtt gaaatcgggg
      841 gttcacgcaa ccctggcggg ggtaattgtc ggcttcttta ttcctttgaa agagaagcat
      901 gggcgttctc cagcgaagcg actggagcat gtgttgcacc cgtgggtggc gtatctgatt
      961 ttaccactgt ttgcatttgc taatgctggc gtttcactgc aaggcgtcac gctggatggc
     1021 ttgacctcca ttctgccatt ggggatcatc gctggcttgc tgattggcaa accgctgggg
     1081 attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg cgcatctgcc tgagggaacg
     1141 acttatcagc aaattatggc ggtggggatc ctgtgcggta tcggttttac tatgtctatc
     1201 tttattgcca gcctggcctt tggtagcgta gatccagaac tgattaactg ggcgaaactc
     1261 ggtatcctgg tcggttctat ctcttcggcg gtaattggat acagctggtt acgcgttcgt
     1321 ttgcgtccat cagtttgaca ggacggttta ccggggagcc ataaacggct cccttttcat
     1381 tgttatcagg gagagaaatg agcatgtctc atatcaatta caaccacttg tattacttct
     1441 ggcatgttta taaagaaggt tctgtggttg gcgcagcgga ggcgctttat ttaacaccac
     1501 aaaccattac cggacagatt cgggcgctgg aagagcgcct gcaaggcaaa ttatttaagc
     1561 gcaagggacg tggtctcgag cccagcgagc tgggggagct ggtctatcgc tatgccgata
     1621 aaatgttcac cttgagccag gaaatgctgg atatcgtgaa ctatcgcaaa gaatccaatt
     1681 tattgtttga cgttggcgtg gctgatgcac tttccaaacg tctggtcagt agcgtgctga
     1741 atgctgcagt agtagaaggt gagccaattc atcttcgctg cttcgaatcc acccacgaaa
     1801 tgctgctgga gcaattaagc cagcataaac tggatatgat catttccgac tgtccgatag
     1861 attctacgca gcaggaaggc ctgttctccg tgagaattgg cgaatgtggc gtgagtttct
     1921 ggtgtacaaa tccaccgccg gaaaaaccgt tcccagcttg tcttgaagaa cggcggcttt
     1981 tgattcctgg gcgacgttca atgttagggc gcaaattact taactggttt aactcccagg
     2041 gattaaacgt agaaatcctt ggcgagtttg atgatgccgc tttgatgaaa gcttttggtg
     2101 cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc atatgacttt tatgccgata
     2161 aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga agagtaccat gctatttttg
     2221 ctgagcggat gattcagcac ccggcggtac agcgaatctg caatacggat tattctgcgc
     2281 tttttagtcc agcggcgcgt taatcggcac ctcccccaaa gttaaggtgg gggagataga
     2341 ttagttgtac ataaccacga ttttgactcg gttcattatt tgcccgcttg agacattatt
     2401 tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat ggggatgctg
     2461 aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat tggagagttg
     2521 aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta atatgctggt
     2581 tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acgtcactgg gttcaaaaac
     2641 taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact gataa
//
LOCUS       DAZUCE010000371         2657 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZUCE010000371 DAZUCE010000000
VERSION     DAZUCE010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.373"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            29..1423
                     /locus_tag="ACHY03_005015"
     CDS             29..1423
                     /locus_tag="ACHY03_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFS3908119.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQTTAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            complement(1464..1778)
                     /gene="rhaM"
                     /locus_tag="ACHY03_005016"
     CDS             complement(1464..1778)
                     /gene="rhaM"
                     /locus_tag="ACHY03_005016"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFS3908120.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(1788..2612)
                     /gene="rhaD"
                     /locus_tag="ACHY03_005017"
     CDS             complement(1788..2612)
                     /gene="rhaD"
                     /locus_tag="ACHY03_005017"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFS3908121.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLDNDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
BASE COUNT          671 a          602 c          707 g          677 t
ORIGIN      
        1 gcctccttta tttcagggaa cgtaaataat gaaaaaatcg acattatctt tagccatcgg
       61 tttattattg gcatgtagta ccggtatggc aaaaacacag catttaacgc tggaacaacg
      121 catggcattg ctggaagaac gcctggaagc ggcagaaatg cgggcggcaa aagcagagag
      181 ccaggttaaa cagctgcaga cacaacaagc cgctgagatc cgcgaaatta aggctgccca
      241 gggcaatacg ccggtaaatg gacagacaac agcggaatct gcaaagaaaa atgccacctc
      301 acctaatctt ttgctttcag gttatggcga tttaaaaatc tacggcgatg tagaatttaa
      361 tatggatgcg gaaagtaatc atggcctgct ggcaatgacc aacgctgatg tgaatagcga
      421 tcccactaat gagcagtgga atctcaatgg tcgtattctg ttaggttttg atggtatgcg
      481 aaaactggat aatggctatt tcgccgggtt ctccgcacaa ccgctggggg atatgcacgg
      541 ttcagtaaat atcgatgatg cggtattctt ctttggcaaa gaaaacgact ggaaggtcaa
      601 agtaggccgt tttgaagcct acgatatgtt cccgctgaat caggatacct ttgttgaaca
      661 ttccggtaat actgcgaacg atctttatga cgatggcagc ggttatatct atatgatgaa
      721 agagggccgc gggcgctcta acgctggcgg taatttcctc gtcagcaaac aactcgataa
      781 ctggtatttt gaattaaaca cgttacttga agacggaaca tctttatata atgacggtaa
      841 ttatcatgga cgcgatatgg agcagcagaa aaatgttgct tatctgcgcc cagtaattgc
      901 ctggtcgccg acggaagaat tcaccgtttc cgcagcgatg gaagcgaacg tggtaaataa
      961 tgcttatggt tataccgata gcaagggtaa ttttgtcgat cagtccgatc gtaccggcta
     1021 tggtatgagc atgacctgga acggcctgaa aacggatccg gacaatggca ttgtggttaa
     1081 tcttaatacc gcctatttag atgctaataa tgagaaagat ttcactgccg ggattaacgc
     1141 gctgtggaaa cgtttcgagc tcggttatat ctacgcgcac aataagattg atgaattcag
     1201 cggtgtagtt tgtgataacg actgctggat tgatgatgaa gggacgtaca ccattcacac
     1261 cattcatgcg tcttatcagt tcgctaatgt gatggatatg gagaacttta atatttacct
     1321 cgggacgtat tactccattc tggatagcga cggcgataaa aaacacggtg atgatactga
     1381 tgaccgttac ggcgcacgcg ttcgctttaa atacttcttc tgatgaaggc gcggtgctgg
     1441 atattttccg gcaccgcaac tcattaaggc aggtaaaaca cttcttgcag ctcgctactc
     1501 accgggctgt tatccgggtt agcgggcata acatcggtca tatatttcca ccaacgttgg
     1561 cagatttcag tgctggctac cgcattccac cgttcttcag attcaatctc taccgtggca
     1621 aacagcagat tacgcgcttt gtcgaggtaa atggcgtagt tatgcgcacc gtgagatttc
     1681 aacactgctt ccagttccgg ccagatggga ttatgccgac gctgatactc ttcgtgggcg
     1741 tcggggttta cctgcatgac aaaggctttg cggatcatgt ttgctcctta cagcgccagc
     1801 gcactggcga gtggtgtaac gccgaaacgc tggccgagcg ctatcaactc ttcacggctg
     1861 atagtctgtt tcatgccgcc catcgaataa accttcacta atacttgtgc tgatttttct
     1921 gcggtgtcga ttaaaccgaa ggtttcatcc agcgtcggtc cggaaccgaa cacaccgtgg
     1981 aagggccaca acaccagcga atgtttttgc atctcttggg cagttgcctg gccgatttcg
     2041 tccgtgccgg gtaccatcca cggcagaatg ccaacgccat ccgggaatac caccagacac
     2101 tcggtgctgc cttcccacag ttggcgagtg aagaccgcgg tgtcgttgtc cagtacatag
     2161 gtgagggcga tcaggttggt ggcgtggcag tgcatgatca cccgatcttt accgttggtg
     2221 gctttaatgc gctcgcagtg ggaaaggaag tgagccggaa gttcagaagt gggaacggct
     2281 tcgttggtta acccccaaag aatgtggtag cccgcgccgt cgctgtcgac ttttacgacg
     2341 cctaagttag ctgcaggatc gagctggacg ttacggaaga atttgccgga gccggtgaca
     2401 ataaacggcg tatttgccag taaaggcatg ggttggctga gcggaatata gcgcggttgt
     2461 gggtggaagt tgtcgtgata aagcgcgata tcggcgtcat ccaggcgcag cgtcaggttg
     2521 ccgccgttgc gctcgtccca gcctttcagc caggcatcgg tggtggcttt gatcattccc
     2581 tggacaaacc aggactgggt aatgttttgc atgttctgtg ttcctgtaaa ttcggtgttg
     2641 tcagatgcac gacctgt
//
LOCUS       DAZUCE010000372         2596 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZUCE010000372 DAZUCE010000000
VERSION     DAZUCE010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.374"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            148..732
                     /locus_tag="ACHY03_005018"
     CDS             148..732
                     /locus_tag="ACHY03_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFS3908122.1"
                     /translation="MQHNSLNAYYQQAFYDVIRNAVDEFPRTLALRVDLRFPRHYQYG
                     DSNKEVTRFIESLKAKLLVDCQRKNLRWKRNRSNRLRYAWVREVGELNRRKHYHVLLL
                     LNKDFYHGAGNYNSDDSLYALIQQAWCSALGLDTEQYTGLANMTENGCFYLNRKLPNY
                     MQQVKELLKRMEYMAKDHTKSYDDGYRSIGMSRR"
     gene            complement(803..1525)
                     /locus_tag="ACHY03_005019"
     CDS             complement(803..1525)
                     /locus_tag="ACHY03_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908123.1"
                     /translation="MDNFSFNDFIVDLKTMFNNKSASLSNRLMAADREQEKVISDANE
                     YHEFVLSLVESMLTNPVVRRRFDDQVYRMSFDEPDTVSETISELVFLADVNWQLGLGL
                     LNTNKNEAIKCLLLAIEYLDYCRGLEDQELWQQQQTTKLDVPAQGGRSKAARFDPIKA
                     KIIRLLQEACPSDGWDTKSEALKSIENGINELKWPSARDQNNLPKTGAEIFAMKIRQV
                     EVWSSQDQKIKAAFDSVVKPKK"
     gene            1640..1867
                     /locus_tag="ACHY03_005020"
     CDS             1640..1867
                     /locus_tag="ACHY03_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3908124.1"
                     /translation="MEPKSVKILRVPALVKKLGIGRSTVYDWLDPSSDRHDPTFPQRV
                     KLGAKTVGWLESEIDAWILSKVKKPLTPVSP"
     gene            complement(2295..2510)
                     /locus_tag="ACHY03_005021"
                     /pseudo
     CDS             complement(2295..2510)
                     /locus_tag="ACHY03_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          717 a          577 c          563 g          739 t
ORIGIN      
        1 tatattaatc atgtaaaacc atacggttaa tgcttactac ccgataatgt tcaggtgcat
       61 atctgcatgc tacattatca tgatggttaa ttgtatggtt atttattctt actcacctca
      121 atattaatta tttattcagg tattaacatg cagcataatt ccttgaatgc ttactaccag
      181 caagcatttt atgatgttat tcgtaatgca gtagacgagt ttccacgtac gttagcacta
      241 cgtgttgatt tacgttttcc ccgccactat cagtatggtg attcgaacaa agaggttact
      301 cgttttattg aatcactgaa agctaagttg ctggtcgatt gccaacgtaa gaacctacga
      361 tggaaacgta accgatctaa tagattacgg tatgcatggg tacgtgaagt aggtgaactt
      421 aaccgccgta aacattacca tgtattactg ctactgaata aagacttcta ccatggtgcc
      481 gggaactata actccgatga cagtttatac gcattaatcc agcaggcatg gtgtagtgcg
      541 ttaggcttgg atacagagca atatacaggt ctggcgaata tgactgagaa cggttgtttt
      601 tatttaaaca gaaaactccc taattatatg caacaggtta aagaattact gaagcgcatg
      661 gagtatatgg ctaaagacca caccaagagt tatgacgatg gatatcgatc aattggaatg
      721 agccgcaggt aacatgcgcg ttgtgagatg gcagtttggc ttttaaggct taaactgcca
      781 gcatattaga cctcaagtac atctattttt taggctttac gacactatca aatgcagctt
      841 taattttctg atcctgactt gaccagacct caacctggcg tatcttcatt gcaaatattt
      901 cagctccggt tttgggcaga ttgttctgat cacgtgcaga tggccatttc agttcattaa
      961 tcccattctc tatgctcttc aaagcttctg atttagtatc ccatccgtct gaggggcaag
     1021 cttcctgcaa aagcctgata attttggctt taatgggatc aaagcgagca gctttacttc
     1081 tacccccctg cgccggaaca tctaattttg tggtctgttg ctgttgccat aattcttgat
     1141 cttcaagacc acgacagtaa tcaaggtatt caatagctaa gagtaggcac tttatcgctt
     1201 catttttatt cgtattcaga agcccaagcc ctagctgcca gttaacatca gccaggaaaa
     1261 caagctcact tatagtttcg ctaaccgtgt ctggttcatc aaatgacatt ctgtagactt
     1321 gatcatcaaa cctacggcgt actaccgggt tcgtaagcat gctttcaacc agcgatagta
     1381 caaactcatg atattcattt gcatctgaaa taaccttttc ctgctcccgg tcagccgcca
     1441 tgagtctatt ggacaagctt gcgctcttat tattgaacat agttttcaaa tcaacgataa
     1501 aatcgttaaa actaaaatta tccattattc aatcagttaa gctccgtaaa aaatgaagta
     1561 ctgcagccgt atgtcgcaca gttgcatcag tagatagtct gacttccatc ctaagcaaac
     1621 caagagagga agctctttaa tggaaccaaa gtctgtaaaa atccttcgtg tacctgcact
     1681 tgtcaagaag ttaggcattg ggcgttcaac ggtatacgac tggcttgacc ctagttctga
     1741 tcgtcatgat ccgacatttc cccaaagggt gaaactggga gcaaagacgg tggggtggct
     1801 agaatccgag attgatgcct ggattttgag caaagtaaaa aaaccgctaa cgcctgtttc
     1861 gccatagttt ggaagattat atgggagaga gtgagtacca cactcttccc attagctttg
     1921 cagacgtgag ccctgtagga ctaaaaccaa ggggcatcac ctccgcctga cggcgagtat
     1981 cttgagcatt gcggtaatca atgttcagcc aagggtcttg gtaaagcgct tcactgaccg
     2041 cgtcaaacat gccttcctgg atcaccggtg gtaggagcgg ctgactggcc gcgaccaccc
     2101 gcaccttctg cggccattcc cactctctca gcgcccgttt gctgtgccta ggaagtgtgt
     2161 cccgcttgac taaaaaacgg cgaaaatgaa gccatcagat gtgcgggtaa cagatgcttt
     2221 aactgcgcct gcgccacttg catcagcggt gattctgctg gcgatagctg cgccaagctc
     2281 aggcctcgcg aacctagcgg aaccgtatgg tcccggttca tcttcattcg agctgcagga
     2341 attttccatt ctttagcttc aaaatcgatc tcatcccaac gagcttctgc cgcttctgcg
     2401 ggtcgtgaga tagttagaag ttgccacatg aacaagcatc gagtaggcaa aataatgttg
     2461 gcatttcgca ttgtctgcat tagctgtggt aactgatcag ggcggatagc ggaggatgaa
     2521 aatgtgaaag ccgcttttga agaagtgatt attggcaaac agtgatttgc gctaaagtta
     2581 tgtaaaccaa tctgaa
//
LOCUS       DAZUCE010000373         2594 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZUCE010000373 DAZUCE010000000
VERSION     DAZUCE010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.375"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            102..1097
                     /locus_tag="ACHY03_005022"
     CDS             102..1097
                     /locus_tag="ACHY03_005022"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFS3908125.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            1340..2233
                     /gene="galF"
                     /locus_tag="ACHY03_005023"
     CDS             1340..2233
                     /gene="galF"
                     /locus_tag="ACHY03_005023"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFS3908126.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELGRTQPGAWGRIQLTDAIAELAKKQSVDATLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT          746 a          542 c          636 g          670 t
ORIGIN      
        1 acactaactg ttaaagctgg ggctgggcag cattgactac tcagggaagg tactcatctt
       61 tatcttgtgt ttcttacgta acaaccagac taaagtgatt tatgaacgat aacgttttgc
      121 tcataggagc ttccggattc gtaggaaccc gactacttga aacggcaatt gctgacttta
      181 atatcaagaa cctggacaaa cagcagagcc acttttatcc agaaatcaca cagattggcg
      241 atgttcgcga tcaacaggca ctcgaccagg cgttagccgg ttttgacact gttgtactac
      301 tggcagcgga acaccgcgat gacgtcagcc ctacttctct ctattatgat gtcaacgttc
      361 agggtacccg caatgtgctg gcggccatgg aaaaaaatgg cgttaaaaat atcatcttta
      421 ccagttccgt tgctgtttat ggtttgaaca aacacaaccc tgacgaaaac catccacacg
      481 atcctttcaa ccactacggc aaaagtaagt ggcaggcaga ggaagtgctg cgtgaatggt
      541 ataacaaagc accaacagaa cgttcattaa ccatcatccg tcctaccgtt atcttcggtg
      601 aacgcaaccg cggtaacgtc tataacttgc tgaaacagat cgctggcggc aagtttatga
      661 tggtgggcgc agggactaac tataagtcca tggcttatgt tggaaacatt gttgagttca
      721 tcaagtacaa actgaagaat gttgccgcag gttacgaggt ttataactac gttgataagc
      781 cagacctgaa catgaaccag ttggttgctg aagttgaaca aagcctgaac aaaaagatcc
      841 cttctatgca cttgccttac ccactaggaa tgctgggtgg atattgcttt gatatcctga
      901 gcaaaattac gggcaaaaaa tacgctgtaa gctctgtgcg cgtgaaaaaa ttctgtgcaa
      961 caacacagtt tgacgcaacg aaagtgcatt cttcaggttt tgtggcaccg tatacgctgt
     1021 cgcaaggtct ggatcgaact ctgcagtatg aattcgtcca tgccaaaaaa gacgacataa
     1081 cgtttgtttc tgagtaatgc agacgagtca gagaaaaaag gaccaggttg gactacgatt
     1141 attcggaaag aggtactgat taaattatca gtatgttgat ttccgagtaa tccgagtaac
     1201 ccagatgctg atggtgtgta gtgttatcgt aaggtttaat caaagttatg cggaactaag
     1261 ctttggtata tagccataac gtttggctaa cctatggcaa taatttacgc actatcagcc
     1321 acttagaggt aggaaaagaa tgacgaattt aaaagcagtt ataccggtag caggtcttgg
     1381 gatgcatatg ttgcctgcca ctaaggcgat tcccaaagag atgctaccga tcgtcgacaa
     1441 gccaatgatt cagtacattg ttgacgagat tgtggctgca gggatcaaag aaatcctcct
     1501 ggtaactcat gcgtccaaga acgcggtcga aaaccacttc gacacctctt atgaattaga
     1561 atctcttctt gaacaacgtg tgaagcgtca actgttggcg gaagtacaat ctatctgtcc
     1621 gccgggcgtg accattatga acgtgcgtca gggtgaacct ttaggtttag gccattccat
     1681 tttgtgtgca cgccccgcca ttggtgacaa cccatttgtc gtggtacttc cggacgttgt
     1741 gatcgacgac gccagcgccg acccgctgcg ctacaacctt gcagccatga ttgcgcgctt
     1801 caacgaaacg ggacgcagcc aggtgctggc aaaacgtatg ccgggtgacc tctctgaata
     1861 ctccgtcatc cagaccaaag agccgctgga tcgtgaaggc aaagtcagcc gcattgttga
     1921 atttatcgaa aaacctgatc agccgcagac gctggactca gacatcatgg ccgttggtcg
     1981 ctatgtgctt tctgccgata tttggccgga acttggacgc actcagcctg gtgcatgggg
     2041 gcgtattcag ctgactgatg ccattgctga actggcgaaa aaacagtccg ttgatgcaac
     2101 gctgatgact ggggacagct acgactgcgg taaaaaaatg ggttatatgc aggcatttgt
     2161 gaagtatgga ttacgcaacc tgaaagaagg tgcgaagttc cgcaaaggta ttgagaagct
     2221 gttaagcgaa taaagatatg acctagtgta gtggctgata aggcaattat ggcggcggag
     2281 gagctcattt agtgtaaata ttcgtgccgt atttttatat ggatatgcag tataacaacg
     2341 attaataaaa ggattctgat cacaaggttt cgcggaaatg attgttctaa aagttaacaa
     2401 tagctaatca gtaagagaat ctagggataa ttttagagga ataggagtat atacaattcg
     2461 ttggaagtgc gtagcgtgct ggtagcttta aagccagggg cggtagcgtt gtattcttcg
     2521 aataattaat cgcaactgtg tgtaatgagt cgttcgttta cgtttaatga atgttaaaaa
     2581 agttagagtc gttc
//
LOCUS       DAZUCE010000374         2562 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZUCE010000374 DAZUCE010000000
VERSION     DAZUCE010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.376"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..284
                     /locus_tag="ACHY03_005024"
     CDS             <1..284
                     /locus_tag="ACHY03_005024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908127.1"
                     /translation="HFVSNSAPVKYNHSIKAAKNYRIINKHAATTHVFSSISNRPYVF
                     YFFTAKSLAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
     gene            238..1902
                     /gene="asnB"
                     /locus_tag="ACHY03_005025"
     CDS             238..1902
                     /gene="asnB"
                     /locus_tag="ACHY03_005025"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFS3908128.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2282..2358
                     /locus_tag="ACHY03_005026"
     tRNA            2282..2358
                     /locus_tag="ACHY03_005026"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2316..2318,aa:Met,seq:cat)
     gene            2367..2451
                     /locus_tag="ACHY03_005027"
     tRNA            2367..2451
                     /locus_tag="ACHY03_005027"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2401..2403,aa:Leu,seq:tag)
     gene            2475..2549
                     /locus_tag="ACHY03_005028"
     tRNA            2475..2549
                     /locus_tag="ACHY03_005028"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2507..2509,aa:Gln,seq:ttg)
BASE COUNT          670 a          636 c          644 g          612 t
ORIGIN      
        1 cgcactttgt cagcaattcg gcccccgtaa aatataacca ttcaataaaa gccgctaaaa
       61 attatcgaat catcaataaa cacgccgcta caacccacgt tttttcatca atcagcaatc
      121 gcccttacgt tttttatttt ttcaccgcca aatcgcttgc gcacagtgct gctttgcggc
      181 atttttttaa acaagcaaac acaacaagca acaaatacca ggttaacgga gaaggttatg
      241 tgttcaattt ttggcgtatt cgatatcaaa acagacgcag ttgagctgcg taagaaagca
      301 ctcgagctgt cacgcctgat gcgtcatcgt ggcccggact ggtccggtat ttatgccagc
      361 gataacgcca ttctcgccca cgaacgtctg tcaattgttg acgttaacgc gggggcgcaa
      421 cctctctaca accaacaaaa aacccacgta ctggcggtaa acggtgaaat ctacaaccac
      481 caggcattgc gcgctgaata tggcgatcgt tatcagttcc agacagggtc tgactgtgaa
      541 gtgatcctcg cgctgtatca ggaaaaaggg ccggaatttc tcgacgactt gcagggcatg
      601 tttgcctttg ccctgtacga cagcgaaaaa gatgcctacc tgattggtcg cgaccatctg
      661 gggatcatcc cactgtatat gggctatgac gaacacggtc agctgtatgt ggcctcagaa
      721 atgaaagccc tggtgccagt ttgccgcacg attaaagagt tcccggcggg gagctatttg
      781 tggagtcagg acggcgaaat ccgttcttat tatcatcgcg actggttcga ctacgatgcg
      841 gtgaaagata atgtcaccga caaaaacgag ctgcgtcagg ctctggaaga ttccgttaaa
      901 agccatctga tgtctgatgt gccttacggt gtgctgcttt ctggtggtct ggattcctca
      961 attatttccg ctatcaccaa gaaatacgca gcccgtcgcg tggaagatca ggaacgctct
     1021 gaagcctggt ggccgcagtt acactccttt gctgtaggtc tgccgggttc accggatctg
     1081 aaagcagccc aggaagtggc aaaccatctg ggcacggtgc atcacgaaat tcacttcacc
     1141 gtacaggaag gtctggacgc catccgcgat gtgatttacc acatcgaaac ctatgatgtg
     1201 acaactattc gcgcttcaac accgatgtat ttaatgtcgc gtaagatcaa agcgatgggc
     1261 attaaaatgg tgctgtccgg tgaaggttct gatgaagtgt ttggcggtta tctttacttc
     1321 cacaaagcac cgaatgccaa agaactgcat gaagagacgg tgcgtaaact gctggccctg
     1381 catatgtatg actgcgcgcg tgccaacaaa gcgatgtcag cctggggcgt ggaagcacgc
     1441 gttccgttcc tcgacaagaa attcctcgac gtggcgatgc gcattaaccc gcaggataaa
     1501 atgtgcggta acggcaagat ggaaaaacac atcctgcgtg aatgttttga gtcatacctg
     1561 cccgcaagcg tggcctggcg gcagaaagag cagttctccg atggcgtcgg ttacagttgg
     1621 atcgacaccc tgaaagaagt ggcggcgcag caggtttctg atcaacagct ggaaactgcc
     1681 cgcttccgct tcccgtacaa cacaccgacc tcaaaagaag cgtatctgta ccgggagatc
     1741 tttgaagaac tattcccgct tccgagcgcc gctgagtgcg tgccgggcgg tccttccgtc
     1801 gcgtgttctt ccgctaaagc gatcgaatgg gatgaagcgt tcaagaaaat ggacgatccg
     1861 tctggtcgcg cggttggtgt tcaccagtcg gcgtataagt aagaaatgcg aaacagcccc
     1921 ggtgaaattc ggggctgttt cagttattga ggtgcttgtt gattctgata tgcagtaagg
     1981 aaatgttccg ggtttgccac atcactgttt gacaactcat cactgtaggc cagtatttca
     2041 tagattaatt tcccaccact attgttaaat tccgaatttc atcctgtatg acaaaagaat
     2101 ggctgtaggg tgggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg
     2161 ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
     2221 gcaaggctct atacgcataa tgcgccccgc aacgccgata aggtatcgcg aaaaaaaaga
     2281 tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga
     2341 atcccgtcgt agccaccatc ttttttgcgg gagtggcgaa attggtagac gcaccagatt
     2401 taggttctgg cgccgcaagg tgtgcgagtt caagtctcgc ctcccgcacc attcaccaga
     2461 aagcgttgta cggatggggt atcgccaagc ggtaaggcac cggtttttga taccggcatt
     2521 ccctggttcg aatccaggta ccccagccat cgaagaaaca at
//
LOCUS       DAZUCE010000375         2551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZUCE010000375 DAZUCE010000000
VERSION     DAZUCE010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.377"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(51..374)
                     /locus_tag="ACHY03_005029"
     CDS             complement(51..374)
                     /locus_tag="ACHY03_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908129.1"
                     /translation="MDCTILLKIISVKEITKNSPLWSHYLARYVDSGYQAEFECEGTA
                     DKWDIYDEWDIYESIADYFDVVGDFNCDVRWSGRKILIKGTCVFPLGEPVKGIHNIPL
                     WIDVE"
     gene            complement(407..1309)
                     /locus_tag="ACHY03_005030"
     CDS             complement(407..1309)
                     /locus_tag="ACHY03_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFS3908130.1"
                     /translation="MSRREKLLKQDLVAHIRKDKGSFKKLSDYTRIMHRFAESLAKMN
                     VQISSAAQVKVRHIELYMRSRSNVSGRTRENEMSAIRVMLRQAGKYKMADPEHPRLSN
                     KALGISGSSRKGTKKLITDERFREILQRVEAKDKGVAAVVKLSRYLGLRNEEAIQSAK
                     SLRTWKKALQRGDEKIRVIFGTKGGRERMTTVVDRDNVLDAVNYALKYASEHNGRLID
                     RDNLKSAMDYYLNTLRRYGELKGGDETAHSFRYSYAASAMKYHIMNGYSYKEALALTS
                     IDLGHGDGRGRYIEQVYCQLLGYE"
BASE COUNT          745 a          627 c          468 g          711 t
ORIGIN      
        1 agcgacatat aagcctgtat atttttaaat cacaaaagat gaatgtcaag ttattcaaca
       61 tcaatccaaa gaggaatatt atgtattccc ttaacgggtt ctcccagagg aaaaacacag
      121 gtccctttaa tcagtatttt tcttcccgac catctgacat cacagttaaa atctcccacc
      181 acatcaaaat aatccgcaat ggattcatag atatcccatt catcatagat atcccattta
      241 tctgctgttc cttcacattc aaactcggcc tgatatccgg aatcaacata tcgcgccaga
      301 tagtgtgacc acaaaggcga attttttgta atctctttta cagagattat tttcagcaaa
      361 atagtacaat ccataatatt tacccgtacg aaatcgccat gagttattac tcatagccta
      421 acaactgaca ataaacctgt tcaatatatc gtcctctgcc atcaccatga ccaagatcaa
      481 ttgatgtcag tgcaagtgcc tccttataac tgtaaccgtt cataatatga tatttcatgg
      541 cacttgcagc gtaactgtac cgaaaactgt gtgcagtctc atctccgcct ttcagttctc
      601 cataacgacg taatgtatta agataataat ccattgccga cttaagatta tctctgtcta
      661 taagacgtcc attatgttca ctggcatatt tgagtgcata attaacagca tcaagaacgt
      721 tatcccggtc aacaaccgtt gtcattcttt cccgtccccc tttcgtacca aaaatcactc
      781 ttattttttc atctccccgt tgtaacgctt ttttccaggt tcgtaatgat ttggccgact
      841 ggatagcttc ttcatttctc agaccgaggt aacgtgacag tttaacaaca gcagccaccc
      901 ctttatcttt tgcttccaca cgctgaagaa tctcccgaaa acgctcatcc gtgattaatt
      961 tttttgttcc cttacgactg gagccggaaa tgcctaacgc tttattgctg agacgaggat
     1021 gttcgggatc agccatctta tatttacctg cctgacgtaa cataacccgt attgcagaca
     1081 tttcattttc ccttgttcgc ccagaaacat tactccggct tctcatatat agctcaatat
     1141 gccggacttt tacctgcgcg gcagaactga tctgtacatt catttttgcc agactttcag
     1201 caaagcggtg catgatcctg gtgtaatcac tcagcttctt gaagctgcct ttgtcttttc
     1261 gaatgtgagc aaccagatcc tgtttcagta atttttcacg tctggacatc aacttctcct
     1321 gcacataata atttttacct taaacagctg tacgttcggc agatgagttt ttctgttgtg
     1381 ccgtgaacac aacagaaaaa ctcatctgca tttctccctg acgacacggt taaacgttgc
     1441 gatccttcgg gtagtgactg cctgtgcgcc agggtagcgc agattgttta ggtattgagg
     1501 ggtaacaaat cggtgttgca caccgactgc agcatttgct gtacctgtct ccaggtcgcc
     1561 cccgacacca cctctaataa gtggtatcgc cgattcgaca tctcactaca taaaactcct
     1621 taaaaaacaa ttgattctgc aaaaaaccat ttatggacgt tattcacaaa atcgtccgta
     1681 aatttgcaga agaggttgaa aaaacactct cacgccgggt cacttcttca aatgttaaaa
     1741 aacatcacgt gaaacggcag ttcgtgcggg ttcgttaagc agacgcgcgt gcgtcacttt
     1801 gtcatcgtga cacgctgacg aaagtgacgc cgcgcagctt cagacgccag atacacgcgt
     1861 aagcgaccaa atttcaaatg gtcttataca gcgcgcacag tcgcaggaag tgctggcaaa
     1921 caaatgaaaa gaataaaaga agtcaatacc gccattacgc agtaagaaca ttccggcatc
     1981 accggatgga taaggatcac acaaccactg ctgtgtattg cccgtaataa agggcgcggt
     2041 taaaagaaac cttcatcgtc atgactgacg atatctgctc tcaaaggtgg gcagatttac
     2101 caccaccaga aacatcctgg ctgcacatcc cctttcaggt tacgggggtg ttgatcaccc
     2161 gaaggcgttc ctctcagatc aacggaacat tggtgcccac cgaagcggga tgtggtcacc
     2221 cgaaggcgct cctctcagac cacttatgga gcattggtgg tcgccgaagc gacagataat
     2281 gcattctgag catatcagag cgcataaaag gcgtcaatta tccatgctcc ctcacgaaac
     2341 agaagctgaa ctcttaacac ctgctatcgg aactcactga ataaacaact aaaaaggaca
     2401 ttgtgaaaat taacatcaac agtatgtaaa gtgatttgtt ttgtcttaaa tgggtgaatg
     2461 taaaaaaata ttctatacaa tccacacatc cttgtatcat atatcaagta aacatccaca
     2521 gagataacag cttatttcaa ataagcagat t
//
LOCUS       DAZUCE010000376         2541 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZUCE010000376 DAZUCE010000000
VERSION     DAZUCE010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.378"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..217)
                     /locus_tag="ACHY03_005031"
     CDS             complement(<1..217)
                     /locus_tag="ACHY03_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908131.1"
                     /translation="MTLFLFVKCKAACVTSLYEVQQSLFTYIYLIRQDPVHFDSYIKN
                     VHNELKIFHDIDKAMNIASDMVKIIDIK"
     gene            complement(298..>2541)
                     /locus_tag="ACHY03_005032"
     CDS             complement(298..>2541)
                     /locus_tag="ACHY03_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS3908132.1"
                     /translation="HSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTL
                     WHYDESDRITHRTVNGEPAEQWQYDGHGWLTDISHLSEGHQVAVHYGYDDKGRLAGER
                     QTVHNPETGELLWQHETEHAYNEQGLANRVTPDSLPRVEWLTYGSGYLAGMKLGGTPL
                     LEFTRDRLHRETVRSFGNNAYELTSTYTPAGQLQSQHLNSLVYDRDYGWNDNGDLVRI
                     SGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTVWPD
                     NRITEDAHYLYRYDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLVFYTRIQHG
                     EPLVESRYLCDPLGRRMAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTDTT
                     RIQTVYEPGSFTPLIRVETENGERKKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLD
                     RLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHLYHCDHRGLPLALI
                     SEDGNTAWSGEYDEWGNQLNEENPHHVYQPYRLPGQQYDEESGLYYNRNRYYDPLQGR
                     YITQDPIGPVGGWNPYSYVLNNPAQFIDPTGLTQEDNWTYGEVGNSCVTSQRDFTPPP
                     GLADMKASIGAACSRNNASRNCHPVDGSNASAPSDKAAWENIQNATNGKDLSGGGNMF
                     CVGNEDCAVVHSCREIVNARHPAGRVRERVTPLTTSGTATLNGTTVYFYNDPRNGNCP
                     AHEYIPQ"
BASE COUNT          494 a          764 c          641 g          642 t
ORIGIN      
        1 tctttatatc aataattttg accatatctg aggctatatt catggcttta tcaatatcat
       61 ggaatatttt taattcgtta tgcacattct taatatatga atcaaaatga acagggtctt
      121 gtctaatcaa ataaatgtat gtaaagagcg attgctgaac ctcatataag gaagttacac
      181 acgctgcttt acatttaaca aataaaaata atgtcataac gcaacatgtt ataaaaaacc
      241 ttttcatgaa aaggatctcc aaaaaaggtg gctagagctt agtctggcca cctttcttta
      301 ttgaggaata tattcgtgag caggacaatt accattccgg ggatcattat aaaagtaaac
      361 tgttgttcca ttgagcgttg ctgttcctga cgttgttaac ggagttacac gttcacgcac
      421 tcgtcctgcc gggtgtcgag cattaactat ttcacgacag ctatgaacaa ccgcacagtc
      481 ctcatttcct acacaaaaca tatttccacc accagataaa tctttaccat ttgtcgcatt
      541 ttgaatattt tcccatgcgg ctttatccga tggtgcagaa gcattactac catctacggg
      601 gtggcagttt ctggaagcat tgtttcttga gcaggcagcg ccaatgctgg ctttcatatc
      661 cgcaagtccg ggcgggggag taaaatcgcg ttgactcgta acacagctat tacctacctc
      721 accataggtc caattgtcct cttgagttaa acctgttggg tcaataaact gggctggatt
      781 attaagcaca taactatagg gattccaccc ccccaccggc ccaatcggat cctgggtaat
      841 atacctcccc tgcaacggat catagtaccg gttgcggtta tagtacagcc ccgactcctc
      901 atcatactgc tgccccggca ggcggtatgg ctgatacaca tgatgcgggt tctcctcatt
      961 aagctggttg ccccattcat catactcccc gctccacgca gtattgccgt cttcgctgat
     1021 gagcgccagc ggcaggcccc ggtggtcgca gtgataaaga tgaacttttc gcgccggtgt
     1081 gtattccggc tccacctgtc tggccagttg ctccaccgtc agcccgcact gcgcaagcca
     1141 cgcccggctt tcactgctca cgcggtctgc ccggatttct tcctccagcc tgtccagcag
     1201 ccgcaccagt tcagccggaa ataccacgcc gtgcccgtcc tcgctccctt cctgctgcag
     1261 cttctccgcc aggctgcggc gctgcgcttt tttccgttcg ccgttctctg tctcgacccg
     1321 gatgagcggc gtgaagcttc ccggctcgta taccgtctgg atacgtgtgg tgtcagtctg
     1381 caccgtcgtc agcctgtcgc cgtcccatcc gtaccatgtc tcctccggtt tacgcgacag
     1441 cgacatccac cccgtcaggt cacgctcccg ccgccagacc cgttttgcca ttcgccgtcc
     1501 cagcgggtca cagaggtagc ggctctcgac cagtggctca ccatgctgta tccgagtata
     1561 aaacaccagg cggtgctgac tgtcgtagtg gtagtggtgg gtccgctcgt cgtccgtccg
     1621 gataacccct gccgggatgc ggtccgtctt ctccgtcagc ctgccgtact catcgtagcg
     1681 gtacaggtag tgcgcgtctt cggtgatgcg gttatccggc cacaccgtga gcgtgctgtc
     1741 aggatgcagc tccgggtccg gcaggcggtt gcccgccggg tcggtggcat acgggatgcg
     1801 gatgtccagg tccggtgcga gggtgcgcac gctctccagc ctgcccgtgg cactgtagcc
     1861 gtattcccgc gtctgtcgcg ggccgctgat gcgcaccagg tcgccgttgt cattccaccc
     1921 gtagtcacgg tcatacacca ggctgttcag gtgctggctc tgtaactggc ctgcgggggt
     1981 gtaagtgctg gtcagttcat atgcattatt gccgaagctg cgcaccgtct cccggtgcag
     2041 gcggtcgcgc gtgaactcca gcagcggcgt cccgcccagc ttcattcccg ccaggtaacc
     2101 gctgccatac gtcagccact ccacccgcgg caggctgtcc ggcgtgacgc ggtttgccag
     2161 accctgttcg ttgtatgcgt gctctgtctc atgctgccac agcagttccc ccgtctccgg
     2221 gttatgcacc gtctggcgtt cgccggccag gcggccctta tcatcataac cgtaatgcac
     2281 cgccacctga tggccttcgc tcaggtggct gatgtctgtc agccagccgt ggccgtcata
     2341 ctgccactgc tctgccggtt caccgttcac cgtgcggtga gtgatacggt ccgattcgtc
     2401 gtagtgccag agggtgacaa gtccctcatc ctcactctgt gtgagttttc cggtcaggtc
     2461 ataatgataa cgctgcgtcc gcccgtcaaa gccgccctgc tgtaccaggc ggtccagcgc
     2521 atcgtagctg aagtcgctgt g
//
LOCUS       DAZUCE010000377         2540 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZUCE010000377 DAZUCE010000000
VERSION     DAZUCE010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.379"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..445)
                     /locus_tag="ACHY03_005033"
     CDS             complement(<1..445)
                     /locus_tag="ACHY03_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908133.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPEIPIFGNSFKYDIE"
     gene            694..1563
                     /locus_tag="ACHY03_005034"
     CDS             694..1563
                     /locus_tag="ACHY03_005034"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFS3908134.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFLTSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(1723..2316)
                     /gene="rclC"
                     /locus_tag="ACHY03_005035"
     CDS             complement(1723..2316)
                     /gene="rclC"
                     /locus_tag="ACHY03_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFS3908135.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNALGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(2328..>2540)
                     /gene="rclB"
                     /locus_tag="ACHY03_005036"
     CDS             complement(2328..>2540)
                     /gene="rclB"
                     /locus_tag="ACHY03_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFS3908136.1"
                     /translation="ATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDAIADIAR
                     KYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT          737 a          510 c          572 g          721 t
ORIGIN      
        1 cttcaatatc atatttaaat gaattgccaa aaataggtat ctcaggaaag ttgactaatc
       61 tacactccgt cttttgctgt tgacgtatta aaaaagaagg gaagatattt gcgagatccc
      121 aataatcacg gtggcggtat acacggggag agcagccgaa taacttttta aattcgcggc
      181 tgaatgactg ctgtgaatca aaatgcaaag agagtgcaat atcaagcata gatttcgcgg
      241 tgagccggac aagaatggcg gcacgacaaa gccttcgttt gcgaatgtat tctcccaaag
      301 gcacatgcat gaaatttcgg aacagaagct ggatgttgcg tctgctgtag ccagatttct
      361 gtgcgatatc ttcgattgaa atagggtgct caagattgca ctcaatccac tccaggagct
      421 gctgtagaat cttctgcctg atcatcacat tctccgttaa ttctttgtca tatcgcacat
      481 tttcgttatg ccctgggccc taagtgtatc aaaaacagca agggcgaagg caaaagcact
      541 ggtagcgtct gcttcgttgt ccccgaaaat agagaggttt cagtcctaca ttattaatga
      601 attttttgca taagtgatat caaaatccac atactaattt gaggttacgt tttaacgtag
      661 actcattgtt catgcctaat ggagggactg acagtggaat tttccgtatt aagtaacaat
      721 ctgaaaatgc cgatgatggg atttggtgtt tttcaggtta ccgataaaaa cgtgtgcaaa
      781 cagtcagtgt tgaacgctat ccgcacgggg tatcgactca tcgacactgc agcggtatac
      841 ggtaatgaag atgccgtcgg tgaagccgtt cgtgaagcta tatctgaagg tttatgtacc
      901 cgagatgagt tatttttaac gtctaaattg tgggtgcagg atatgttgaa tcaggatacg
      961 gcagcagcag gtattgaagc atcattaaaa aaatcgggac tagaatactt cgacctctat
     1021 ttattgcatc aggctatgcg cgattatttc agtgcgtggc gtgcactcga agatgcctat
     1081 gaagagggca aattaaaagc gattggagtt tccaatttct atcctcatgt tctggcgaac
     1141 ttttgtgaaa cggtaagagt taaaccgatg gtcaaccagg tcgagttgca tccctatttt
     1201 gcccaaccag aggcgctggc aaccatgaag tattataacg tgcagcctga agcatgggct
     1261 ccgttaggtg gtggacgaca taaacccttt gaaaataatc tgcttcagag tattgcagat
     1321 gcccatcaaa aatcgatttc tcaagtcatt ctgcgttgga atattcaacg gggagtggtc
     1381 gttattccga aatcgacaca tcaacagcgt atcgaagaaa attttgctat ctgggatttc
     1441 tcactgacag agaaagaaat ggcacaaatt agttcgcttg atttgggtta tgttggggag
     1501 tcggtaaaac attttaatcc tgaatttgtt cgtggttgtc ttgctgtaaa aatacatgat
     1561 tgatattaat catgtatttt acctgagacg acaagaatct tttaacaggg gagtgatatt
     1621 gatcttcact ctgtcatatc tccggtaata tggcgtcagg cttcatcatc agaaaggggg
     1681 cctgacgcct gaaaaagtga acaacagaca gtgttcggat tatcaatatt cagtttttaa
     1741 agttgaactg gattcattac tgcgttgttt aagaatttcc cgcgccgaat ctgccattat
     1801 catgacagca cctgccagca tcagagtatc tttcaatacc aagcgaccag caccggataa
     1861 ataagggaaa ccatgatgag cgtcacccaa tgcgggtacc catgcctccg gggtggtgat
     1921 taaaaatgag agtgttacca acggtgtggt aaatgccatc agccctccca ataaacctaa
     1981 ccagcgattg acaggattag ccaaaaccag cagagcaata atcacctcca cgacgccaag
     2041 agcgttggaa aaaccatagg tattattggc cgattgccat gcccttgctt ctggtttgta
     2101 ttcgccttcg tgagtcagat actgtttata gtcttccggg tgttcataaa agaacgacat
     2161 tagtggactg tttgcgacga atggtgtaat gctgtctgcc tcgtaaggga caaactttaa
     2221 taacccaatc cacataaaaa caatcgcaat actcagacga atcaatgtca ggccaatttt
     2281 atctccccga cttaacaggt gcaggtattt ttccattgtg aacatcctta tttatacaat
     2341 actgctgttg cggttgaatt attatcaatt cgcatcgatg tgactttcca ggatgaagca
     2401 ttatattttc tggctatatc agcaatggca tcctccagaa ctgtcggtga tgctgatact
     2461 gacgagacgc tgatatgttt cagaattatt ttatcatctg cattggtgga aaatgccata
     2521 acgccagata atagtgttgc
//
LOCUS       DAZUCE010000378         2478 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZUCE010000378 DAZUCE010000000
VERSION     DAZUCE010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.380"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..728
                     /locus_tag="ACHY03_005037"
     CDS             <1..728
                     /locus_tag="ACHY03_005037"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908137.1"
                     /translation="THNPLFVDRQDIKSNIIIDKGAATPAKSIKQIRDLLGIKASDNL
                     TNANFVLVVEGEDDVIALTALFKILSNKLRQMIESHMLIVEPIGGAGNLTYKLSLLKN
                     SLCVYHTFLDNDDAGRNAYAAAEQENLLNIKSNTFVNCNGSRNSEFEDCLDPASYKEK
                     IKEEYGVNLDCKEFRGNDKWSDRVRKAFLAQGKQWNNNVEAKVKYTVANCIKKNPENA
                     LSQHKRNSIDALVESLEQMIGGK"
     gene            complement(778..1965)
                     /locus_tag="ACHY03_005038"
                     /pseudo
     CDS             complement(778..1965)
                     /locus_tag="ACHY03_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          679 a          545 c          633 g          621 t
ORIGIN      
        1 ctacacacaa tcctttattt gtggatagac aagatattaa atcaaacata ataatagata
       61 aaggtgctgc tacacctgca aaaagtatta agcaaatccg tgatctactt ggtataaaag
      121 catctgataa tctgactaat gctaattttg ttctggttgt tgaaggcgaa gatgatgtga
      181 ttgcgcttac agcattgttt aaaattttaa gtaataaact caggcaaatg attgaaagtc
      241 atatgttgat agtcgagcct ataggtggag ccggcaatct aacatataag cttagccttt
      301 taaaaaactc tctgtgtgtc tatcatacgt ttttagataa cgatgatgca gggagaaatg
      361 cttatgccgc agctgaacaa gaaaatcttc ttaatatcaa gtcaaacact tttgttaatt
      421 gcaatggttc aagaaactcc gagttcgaag actgtttaga tccagcttcg tacaaagaga
      481 aaatcaagga agaatatgga gtaaatttag attgcaaaga atttagagga aatgataaat
      541 ggagtgatag agtgagaaaa gcattcttag ctcaaggtaa gcaatggaac aataacgtag
      601 aagcaaaagt gaaatataca gttgcaaatt gtataaagaa gaaccccgag aatgccctaa
      661 gccaacataa acgtaattct atagatgctt tagtggaaag tttggaacaa atgattgggg
      721 gtaagtaaac gattattggg ggggccccta ttaaattgtc aagcatgttt atgatgccta
      781 cggggtataa aaagtcccgt cacgcatcat ggcgaacaaa acgtcacagc gccgtcttgc
      841 gagagcgata agagtctagt tgtgtcgttt cccttggttt attttgcgtg tgtagtaagc
      901 tttagagatc ggctccctga gcgcagcgaa ggctgacagg aacaacgccc ccttgagggt
      961 tttattgccc cgtcttgaca ggtgttcgcc acgaattgac gagccggaac gccgggttat
     1021 cggtgcaagg ccagcataag cagcaagatg tgctgcagag gcgaaggccc gacaggcaac
     1081 ctctgtgagg agcctggctg cggccctgac accgactccg ggcatgctgg tcaggaccgg
     1141 gtaaagaggg tgaacaagca ccctctgttc tacctcaaga gccacctcgt ctctttgctt
     1201 acgcagtgtg atgagctgga gtgccagacg cgggagaacg acgatagcag catttgtgcc
     1261 gggaacgacg acggtttgct cagtcagggc tcgggttatg tcagcagcga gactctttcc
     1321 cagacggggc gcgagtttac tgagttgggc tgccagcttc ttctcactca gcgaagccag
     1381 tttttctggt gagggatatc tctggagaag ttcaagtact gccggatgat ccagacacgg
     1441 gccgagaacg cgctccagag ccggatgtat ctggttcaac aaaccgcgga tacgattgct
     1501 ggcctgcatt gtctgtgcgg caagatcatc atcgaagcca cagagcatgg agagttctgc
     1561 gatttgctcg tcagccacct tcagcgtgcg taatgcatga gctagagttc gggccgcttc
     1621 ggcgatggca gccgcatcgc gggcatcgtt tttagcttca ccggcgtgca agtcggcgat
     1681 gcgtcgcact gccagtccgg gaaggtatcc gacaaggacg ccttcagagc gggcaatggc
     1741 gacgggtaac gctccgatgg tagcagactg gtcaacgacc agcagtatct gtccatgctg
     1801 tttcaggtca gatatcagcg ctctgagttt gttttcgtcg ttgggtaatg ctctgtcgaa
     1861 caaacgttta cctgaacgat taacggcaac ggcgtgatgg gtgtctttac ctacatcaac
     1921 gccaataaag acctggattg attcgtaatc gctggattcg gtcattgtgt ctccctgttt
     1981 atacgggtta accagataac cttgggcagc aggtaccggc atccacgtta cagacggtcc
     2041 cgacatgatg tctgacctgg cccctatcag cggttaccag cgccccacca gacccggtga
     2101 catccccccg gatcatgaac gactgggggc agtaatcatg ccgggtctgg ctggctaaca
     2161 ccccattata aggggtacga ataaagtaac ggggcaattc tttcattgtg caatacattt
     2221 gattgtgcgg ctttgacatc cgctcctcac tcacattgaa ccttcagctt agttaacacg
     2281 tccgctccga gtcagaagcg gatcttagac ttcagtaatt tggttagccc cccattttaa
     2341 taaactacag ctgcagtact acttttataa tgagcgtagt ttcaataagg cacccaaaac
     2401 gttagtcagg aaaagccgac tgaaaggata tatgtgggca agtagtattt atttaatgcc
     2461 atttttcgag aaaatgag
//
LOCUS       DAZUCE010000379         2449 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZUCE010000379 DAZUCE010000000
VERSION     DAZUCE010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.381"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            264..989
                     /locus_tag="ACHY03_005039"
     CDS             264..989
                     /locus_tag="ACHY03_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908138.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            947..1624
                     /gene="bglJ"
                     /locus_tag="ACHY03_005040"
     CDS             947..1624
                     /gene="bglJ"
                     /locus_tag="ACHY03_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFS3908139.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1662..>2449)
                     /gene="fhuF"
                     /locus_tag="ACHY03_005041"
     CDS             complement(1662..>2449)
                     /gene="fhuF"
                     /locus_tag="ACHY03_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFS3908140.1"
                     /translation="AYRSAPLYEDIIWRTHLQPQDARLAQAVRATIAEHREHLLEFIR
                     LDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGLM
                     VPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETLI
                     SQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEKT
                     LTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
BASE COUNT          639 a          539 c          602 g          669 t
ORIGIN      
        1 atgtgatttc tgtttttcat taagtattcc taacaactta atgtagatat tttaaatgtc
       61 tccaggctat ttcaccaggt tatcatttga tgctaacctt ctgacacatg cagtggagtt
      121 gttgtgcagc aggagtatgc tgatatgaaa gtgaatgcta aggataattt attcgcttaa
      181 tctattaatt tgctggaaac atttaaggaa tgaatatcct gaataaacca tcataatttt
      241 tagacagtgg atgtggagga aatatgttgc caggatgctg caaaaatgga attgttatca
      301 gtaaaatacc tgttatgcaa gcagggttaa aagaggtcat gaggactcac ttccctgaat
      361 atgaaataat atccagcgcc tctgcggagg accttacctt attacaatta cgtcgttccg
      421 gattagtcat tgctgattta gccggtgaaa gtgaagatcc acgttctgtt tgtgaacatt
      481 attattcttt aatctcacaa tatcgggaaa ttcactgggt tttcatggtg tcacgttcct
      541 ggtattccca ggcagtagaa ttgctcatgt gccctacggc gacgttattg tctgatgttg
      601 aacccattga gaatctggtc aagaccgtac gttccggcaa tacgcacgca gagcgtatca
      661 gcgccatgct gacctccccg gcaatgactg aaactcatga ttttagctat cgctccgtca
      721 ttctcactct ttcagagcgc aaggtactgc ggctattagg taaaggatgg ggcatcaacc
      781 agatagcttc attgcttaag aaaagtaata aaactatcag cgcccaaaaa aacagtgcga
      841 tgcgtcgact ggcaattcac agcaacgctg aaatgtatgc atggataaat agcgcgcagg
      901 gtgcaagaga acttaacttg ccttctgttt atggagatgc cgcagaatgg aacacagccg
      961 aattaagaag agaaatgtcg cactcataga aaaatgcgtc atgagtagta tcggtattga
     1021 gagtttattc agaaagtttg cgggcaaccc ttataagctc catacctata ccagtcagga
     1081 gtcatttcag gatgccatgt cgcggatctc gtttgcggcg gtcatttttt ctttttctgc
     1141 catgagaagt gagcgcagag agggattatc ttgcctgact gaactggcga ttaagtttcc
     1201 gcgtacccgg cgtttagtta ttgcggatga tgatattgaa gctcggctga ttggttcatt
     1261 gtcgccatca ccgctggacg gtgtattaag taaagcgtca acgctggaga tttttcatca
     1321 ggaactcttt ttgtcattaa atggtgtacg tcaggcgacc gaccgactga acaatcagtg
     1381 gtacattaac caaagccgga cgttaagccc gacggagaga gaaatattgc gctttatgtc
     1441 gcgtggctac tcaatgacac aaattgccga gcagcttaaa cgcaatatca aaacgatccg
     1501 tgcacataaa tttaatgtga tgtcgaaact gggcgtcagt tctgacgcag ggttgttgga
     1561 ggccgcagat attctgttat gtatgcggca ttgcgaaaca agtaatgtgt tgcatcccta
     1621 ttaatccgca tgatgccggg tttacttccc cggcagtgct ttcatttcag cgtacaatcg
     1681 ccacattgct gcacatccgg taagcgataa cgctggcagc aagtgcggcg caccagcagg
     1741 ccgtcgcgta gcaccacggt acgccagagt ggattatctt caccgttcgt gagcgttttc
     1801 tcaaaaaaga gggcatggcg cagcgattca accgtcgcct cgccgagcag ttgcttcatc
     1861 tcagtgagat accagttgat caaataaccg gtattactcc agataagttt gccgttgatc
     1921 tctccggtcg cttctagtgc ttgcacaacc ggaaccagcg cctggctgat taacgtttcc
     1981 attcgctgct gcggcgaatg tggtgttgcg tttttatctt cacacacatc gacccagaaa
     2041 caggcgacgc gtccggtttc gtgaaactca gcatggaaat gttccggcga cacatctaat
     2101 gctttttcct gcgtcagtag cgccagcatt aatggtggca ccatcaggcc gatataccat
     2161 tgtgcccata gtgagatcag cggtttgttc tcgcggatca tcgttggttg gttgcgatag
     2221 atatgatcgg aatagaccgc cagtagagaa cttagcgcat tcggtgatga ccattgcgcc
     2281 agagtcatgg cgttaagcgg agcgggttca tccaggcgga taaactcgag caaatgttca
     2341 cgatgttcgg cgatcgtcgc acgtacggct tgtgcaagcc tagcatcctg tggctggaga
     2401 tgcgttcgcc agatgatatc ttcatagagc ggtgcggaac gataggcca
//
LOCUS       DAZUCE010000380         2416 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZUCE010000380 DAZUCE010000000
VERSION     DAZUCE010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.382"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..2235
                     /locus_tag="ACHY03_005042"
     CDS             <1..2235
                     /locus_tag="ACHY03_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFS3908141.1"
                     /translation="HSVFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTL
                     WYYDESDRITHRTVNGEPAEQWQYDGHGWLTDISHLSEGHRVAAHYGYDDKGRLTGER
                     QTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPL
                     LEFTRDRLHRETVRSFGSMAGSNAAYELTNTYTPAGRLQSQHLNSLVYDRDYGWSDNG
                     DLVRISAPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTL
                     TAWPDNRITKDAHYLYRYDEYGRLTEKTDRIPTGVIRTDDERTHHYHYDSQHRLVFYT
                     RIQHEEPLVESRYLYDPLGRRTGKRVWRRGRDLTGWMSLSRKPEVTWYGWDGDRLTTV
                     QTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQCRSLAEKLQQEGSEDGHGVVFPAEL
                     VGLLDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDYRGL
                     PLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYD
                     PLQGRYITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDI
                     LSDTYEDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFG
                     MYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKKTIKALQDA
                     GYLK"
     gene            2216..>2416
                     /locus_tag="ACHY03_005043"
     CDS             2216..>2416
                     /locus_tag="ACHY03_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908142.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFPHVA"
BASE COUNT          624 a          597 c          735 g          460 t
ORIGIN      
        1 cacagcgtct tcagttacga tgcgctggac cggctggtac agcagggcgg ctttgacggg
       61 cggacgcaac gttatcatta tgacctgaca ggaaaactca cgcagagtga agatgagggg
      121 cttgtcaccc tctggtacta cgatgaatcg gaccgtatca ctcaccgcac ggtaaacggt
      181 gaaccggcag agcagtggca gtatgacggc cacggctggc tgacagacat cagccatctc
      241 agtgaaggcc accgtgtcgc tgcccactac ggctacgacg ataagggccg cctgaccggc
      301 gaacgccaga cggtggagaa cccggagacg ggggaactgc tgtggcagca tgagacgaaa
      361 cacgcataca acgaacaggg gctggcaaac cgcgtcacgc cggacagcct gccgccggtg
      421 gagtggctga cgtatggcag cggttacctg gcgggaatga agctgggcgg gacgccgctg
      481 ctggagttca cgcgcgaccg cctgcaccgg gagacggtgc gcagcttcgg cagcatggca
      541 ggcagtaatg ccgcatatga actgaccaac acatacaccc ccgcaggccg gttacagagc
      601 cagcacctga acagcctggt atatgaccgt gactacgggt ggagtgacaa cggcgacctg
      661 gtgcgcatca gcgccccccg gcagacgcgg gaatacggct acagcgccac gggcaggctg
      721 gagagcgtgc gcaccctcgc accggacctg gacatccgca tcccgtatgc gaccgacccg
      781 gcgggaaacc gcctgccgga cccggagctg cacccggaca gcacgctcac ggcgtggccg
      841 gataaccgca tcaccaaaga cgcgcattac ctgtaccgtt acgatgaata cggcaggctg
      901 acggagaaga cggaccgcat cccgacgggt gtgatacgga cggacgacga gcggacccac
      961 cactaccact acgacagcca gcaccggctg gtgttttaca cgcggataca gcatgaggag
     1021 ccactggtcg agagccgcta cctctacgac ccgctgggcc gccggacggg aaaacgggtg
     1081 tggcgtcgcg ggcgtgacct gacgggatgg atgtcgctgt cgcgtaaacc ggaggtgacg
     1141 tggtacggat gggacggcga ccggctgacg acggtacaga ccgacaccac gcgtatccag
     1201 acggtatacc agccggggag cttcgcgccg ctcatccgca tcgaaacaga caacggtgaa
     1261 cgggagaaag cgcagtgccg cagcctggcg gagaagctgc agcaggaagg gagcgaagac
     1321 ggccacggtg tggtgttccc ggctgaactg gtggggctgc tggacaggct ggagggggaa
     1381 atccgggcaa actgcgtgag cagtgaaagc cgccagtggc ttgcgcagtg cgggctgacg
     1441 gtggagcggc tggcggcaca gatagagccg gtttatttgc ctgagcgaaa aatacatctt
     1501 tatcactgtg actaccgggg cctgccgctg gcgcttatca gtgaagacgg caatacggcg
     1561 tggagcgcgg aatatgatga atggggcaac cagcttaatg aggagaaccc gcatcacctg
     1621 caccagccgt accggctgcc ggggcagcag tatgataagg agtcggggct gtactacaac
     1681 cggaaccggt actacgatcc gttgcagggg cggtatatca ctcaggaccc gatagggctg
     1741 gaggggggat ggagtctgta tgcgtatccg ctgaatccgg tgaatggtat tgatccatta
     1801 gggttaagtc ccgcagatgt agcgctaata agaagaaaag atcaactaaa ccatcaaaga
     1861 gcatgggata tattatctga tacttatgaa gatatgaaga gattaaattt aggtgggact
     1921 gatcaatttt tccattgtat ggcattttgt cgagtgtcta aattaaatga cgctggtgtt
     1981 agccgatcgg cgaaagggct gggttatgaa aaagagatta gagattacgg gttaaatctg
     2041 ttcggtatgt acggcagaaa agtaaagcta tctcattctg aaatgattga agataataaa
     2101 aaagacttgg ctgtaaatga ccatgggttg acatgtccat caacaacaga ttgctcagat
     2161 agatgtagtg attatattaa tccagagcat aaaaaaacga taaaggcttt acaagatgct
     2221 ggctatctca agtaatctat caaagatgat aatatttatt tttgctatta taatcattgt
     2281 tgttttatgc gtaattactt atctttattt atacaaagat gaatctcttg taagtaaaca
     2341 ttacataaac tatatggcaa taccagaaaa tgatggagtt tttacatggc tcccagattt
     2401 ttttccgcac gtagcg
//
LOCUS       DAZUCE010000381         2399 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZUCE010000381 DAZUCE010000000
VERSION     DAZUCE010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.383"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(44..246)
                     /locus_tag="ACHY03_005044"
                     /pseudo
     CDS             complement(44..246)
                     /locus_tag="ACHY03_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(203..395)
                     /locus_tag="ACHY03_005045"
                     /pseudo
     CDS             complement(203..395)
                     /locus_tag="ACHY03_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(392..862)
                     /locus_tag="ACHY03_005046"
     CDS             complement(392..862)
                     /locus_tag="ACHY03_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908143.1"
                     /translation="MATILTPDSDLLDLLTAPFNATTDFTVLADYCARFAETLIETND
                     PALRLALCGRLHAALALLQPTLHDPIPPHLTESLTVDTLPATPEHFISEPDSLCEYCL
                     ALLQLLLERPAEPKMEQTLTGLLCELVWYFTEGLKAPRWLRTADGVKFISEVAA"
     gene            complement(1209..>2399)
                     /locus_tag="ACHY03_005047"
     CDS             complement(1209..>2399)
                     /locus_tag="ACHY03_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908144.1"
                     /translation="FLKSTKNTRLEKVAKGNRKNESYLPYIFPTRPLTPAEISELAPE
                     CVGLPSTSDKKPDERPSTIWAKYTQALRGVWIKPTLLASEQDSDEATKKARPKKFIHI
                     GTDRKHKVVVALTSIKTEEDDWAKMACNKSNLSRSRYQRISELVNATLKLYPKPDYVL
                     FPELSIPLRWVNSIADRLSSAGISLIAGTEYRHLDDNQLKSEAVLVLSDNRLGYPASV
                     KIWQPKLEPAVGEDEALFSTYGKSWDSTLNVKQRKPVYIHHGVNFGVMICSELQNSKA
                     RIRFQGALDALMVLSWNKDLDTFASLIESAALDIHAYTILVNNRKYGDSRVRSPAKEP
                     FMRDIARVKGGDNDFVVAATLDIDSLRAFQSRAKRWPEGGDKFKPLPEGFQLAKNRKK
                     LPPK"
BASE COUNT          567 a          575 c          606 g          651 t
ORIGIN      
        1 cgtaggttcg actcctatta tcggcaccat ttaaatcaag tagttacccc atattcaaat
       61 acaccacgtt tcctcctgtg ccgtatttgt gccattgcga cttatgatcg tgccgatttt
      121 gcttgcgtgc tcggtgagat gcccggctga aaggtgagcg tatctctgaa cctttttgag
      181 agttttccat cctcccatct ctttaagtgc aagaagagag actccggact gaaccagcca
      241 gcttgccttt gctatcaaag taccttaagg accagatgat ttctggcgat atttacagcg
      301 cttccgcaat gagtttttct ctgtgccgcc agagacgttc cccgcttttt cagcaccgca
      361 ataatgtcgg cggaatgcca gtcctgttgc ctcatgcggc cacctcactg ataaatttta
      421 cgccgtcggc ggtacgcagc cagcgcgggg ctttgagtcc ttccgtgaaa taccagacca
      481 gctcacacag cagcccggtc agggtttgct ccatttttgg ctccgccggg cgttccagca
      541 gaagctggag cagtgcgagg cagtattcgc acaaggaatc gggttcggaa ataaagtgct
      601 ctggtgtggc gggaagggta tcaacggtca ggctttcggt aagatgcggc gggatggggt
      661 cgtgtagtgt gggttgcaat agcgcaagag ccgcgtgaag ccgcccgcag agcgccagtc
      721 gtagtgcggg atcgttggtt tctatcaggg tttcggcgaa gcgggcgcag tagtcggcaa
      781 gaacggtgaa atccgtggtg gcattgaagg gggcggtcaa tagatcgagt aaatcgctgt
      841 caggggtgag gattgtagcc atagggcaac ctccgataag tgggggttca agccaccacc
      901 ggagaggcta atctcgcggg tggtggaacc gggcagggtt agccttacca gccttatcgg
      961 acactggcgc gtcttgcgac gcccccgccc ggcccaccat tgagatgtaa ccgtgcgaac
     1021 aacacaaaaa aagacgctgg cgcgtcatgt gtcgccgata agatttcttc aggaggctaa
     1081 tcccgacgcc tgattttgca ggcgcgggag aaagatactg cgattggtca tgagtggcaa
     1141 gggctacatc atgtttaccg acatctcacg caaaaatacc ctgattatta atagcgaaaa
     1201 tcagtttctt attttggcgg tagctttttg cggttctttg ccaactggaa tccttcaggt
     1261 aacggtttga atttatcgcc gccttcaggc cagcgttttg ccctgctctg aaatgccctt
     1321 aacgagtcga tatccagcgt tgcagcgacc acaaagtcat tatcaccgcc cttcacacga
     1381 gcaatatcac gcataaaggg ttcttttgcc ggggaacgta cgcgactatc gccgtatttt
     1441 cggttattca ctaaaatagt ataggcatga atatccagcg ctgctgattc aatcaacgat
     1501 gcaaacgtat ctagatcttt attccagctc aataccatta atgcatcgag tgcgccctga
     1561 aaacggatcc tcgctttact attctggagt tcagagcaaa tcataacgcc aaaattgacg
     1621 ccgtgatgaa tatataccgg cttacgttgt ttaacattaa gtgtcgagtc ccaagactta
     1681 ccataagttg aaaataatgc ctcatcttca cctacggcgg gttccagctt gggttgccat
     1741 attttgacac tcgctggata gccgagtctg ttatctgaaa ggacaagtac ggcctcactc
     1801 ttcagttgat tatcgtctaa gtggcggtat tctgttcccg caattagact gatacccgcc
     1861 gaactcaaac gatcagcaat actgttaacc cagcgtaacg ggattgaaag ctcagggaat
     1921 aaaacataat caggtttagg atatagtttc aatgttgcat taaccagttc agaaatccgc
     1981 tggtaccttg aacgggacaa gttagattta ttgcaggcca ttttagccca gtcgtcctcc
     2041 tctgttttaa tgctggttag cgcaacgaca actttatgtt tcctgtctgt gccaatatga
     2101 atgaatttct taggccgagc tttttttgtc gcttcatctg agtcctgctc cgatgctagc
     2161 aacgtcggtt tgatccatac tccgcgtaat gcttgagtat attttgccca aatggtggac
     2221 ggtctctcat ctggtttttt gtcggatgtg gagggtaatc caacacattc gggggccagt
     2281 tctgatattt cagcgggtgt taaaggacgt gtagggaaaa tgtaaggtag gtaactttcg
     2341 ttcttacgat ttcctttagc cactttttcc agtcgggtat tcttggttga tttcaaaaa
//
LOCUS       DAZUCE010000382         2396 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZUCE010000382 DAZUCE010000000
VERSION     DAZUCE010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.384"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(303..2156)
                     /locus_tag="ACHY03_005048"
     CDS             complement(303..2156)
                     /locus_tag="ACHY03_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013318588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4209 domain-containing protein"
                     /protein_id="HFS3908145.1"
                     /translation="MKNERYPLDLAISVQDFNTSDWKDSIAQATREGYSAMWQALSDA
                     ARDAIEQGRIEHGKVLWLLADACSMMLSPSSPNEPFKPFAVFHDRRSVVPDDISDSDI
                     LFFAEIVDGVDNDWLKARLSDLVWLKSKPRNIEFALKAIDAYRSLPLDTDTWIHGGRD
                     CWSRAISLARMLKGGAGDRLQQIETTIVAAFHASIRDDGFLGLWLADLLKSNRLGGAH
                     RTDVSTKLEALARGFDDEGDLHKAREYFSAAAEWYKLIPDEAKAAEMTVLVAEGWVKE
                     AVARVASESPSHMVAASFYENAIQIYRTIPRAERSTHQVDERIAELRVHLNNSGERAI
                     GEMGLIQTPGVDLTQVIEKARESVTGKSAQNALLAFANLHRGVNAEELRNSALERMRQ
                     HPLQSLFAVTVMSRDGRVIAKRPAMGLGGELTEDDEIAIRAEMIRDYGILVSIVVQAH
                     IWPALEVLLLEHRLRESDFIELARHSPIVPKERVGLFGKGLFAGYERDFVTALHLLIP
                     QIEHLVRVHLKQAGTKTTNLDKDGIENENGMSTLVGLPEAEQVFGKDLVFEFESLFCN
                     AFGPNLRNELAHGLLDEDGCNSPFAIYAWWLALRLTFNTWWNSANPISEPQ"
BASE COUNT          635 a          635 c          507 g          619 t
ORIGIN      
        1 atatctattc ttttgttcta acgtcaacac ctacccttaa aaaaataatt ctctaagaat
       61 gagagaatta ttgtgtgtat tagataagat atgagctgac acatctatgg cgaggagccg
      121 aatttaatct gacagtccac tttgtgccag aagcaggcac tacaaacatc gcggtgtgtg
      181 attgaatggg gtgagcagtg tatacacagc tcccccctac atttaggtta ataaagaatt
      241 ctacttatgc acacgccgct ttttcagagg ggttactagg ccactgcttg ctcgtcgcta
      301 ccttattgag gctcagaaat aggatttgct gagttccacc atgtattgaa tgttagtctt
      361 aaagctagcc accacgcata aatagcgaaa ggggaattgc acccatcctc atccaatagt
      421 ccatgagcca attcgttccg caaatttgga ccaaaggcgt tacagaacaa cgattcgaac
      481 tcaaagacga gatctttacc aaatacttgc tcggcctctg gcaaacccac caacgtgctc
      541 ataccattct cgttctcaat tccatccttg tcgaggttag tggttttggt tcctgcttgc
      601 ttaagatgca cgcgcaccaa gtgctcaatc tgagggatca gtaaatgaag cgcggtaacg
      661 aaatcacgtt cgtaacctgc aaaaagtccc ttgccgaaaa ggccaactcg ctcttttggc
      721 acaatgggtg aatggcgagc taactcgata aaatcgctct cgcgcaaacg atgctcaagc
      781 aaaagtactt caagggctgg ccagatgtgg gcttgaacca caatactaac taagattcca
      841 tagtcacgaa tcatctcggc gcgaattgca atttcgtcgt cctctgtcaa ttctccaccg
      901 agtcccatcg cagggcgctt ggcaatgacc ctgccatcgc gactcatcac ggttacggca
      961 aacagtgatt gcaagggatg ctgacgcatt ctttccaatg cactgttgcg cagttcttct
     1021 gcattcacgc cacgatgtag gttcgcaaat gcgagcaacg cattttgtgc agatttcccg
     1081 gtgacggatt ccctcgcctt ttcgataacc tgagtgagat ctactccagg agtttgaatg
     1141 agccccatct ctcctatagc tctctctccc gaattgttta ggtggactcg caactcggca
     1201 attcgctcat cgacctgatg tgtagagcgt tcggcccgag gaatcgttct gtatatctga
     1261 atggcatttt cgtaaaagct cgctgccacc atgtggctag gactttccga tgcaactcgc
     1321 gcaacggctt ctttgaccca cccttcagct accaatactg tcatttctgc agctttggcc
     1381 tcgtcaggaa taagcttata ccactcggca gcagcagaaa aatactcccg cgccttatgc
     1441 aaatcccctt catcatcaaa cccgcgggcc aacgcttcta gcttcgttga cacatccgtt
     1501 cgatgtgccc cccccaagcg gttcgatttt agaaggtcgg ccaaccaaag cccaagaaaa
     1561 ccgtcgtctc gaattgacgc atgaaacgcg gcaacgattg tcgtctcaat ttgctggagt
     1621 ctatctccag caccaccttt tagcattcga gccaagctta tggcgcgaga ccaacaatcc
     1681 cgccctccat gtatccatgt atcagtgtct agtggaaggc tgcggtaagc gtcgatagcc
     1741 ttcaaagcga attcgatgtt acgaggctta ctcttcaacc agaccaaatc actgagtcgc
     1801 gccttcagcc aatcgttgtc taccccatca acgatctctg caaagaacag aatatctgaa
     1861 tcagaaatgt catcagggac gactgacctg cggtcatgaa agacagcaaa aggcttaaag
     1921 ggttcattag gactcgacgg cgaaagcatc atcgaacagg catctgcaag caaccataat
     1981 accttgccgt gttcaattcg tccttgctca atggcatcac gcgcagcatc cgacagcgct
     2041 tgccacatcg ccgaatatcc ttcgcgggta gcttgggcaa ttgaatcctt ccagtctgac
     2101 gtattgaagt cctgaaccga aatagccaag tccaacgggt aacgttcgtt cttcattatt
     2161 tatcaccttt aaccgatgca acattcattc gtagttcgtc aaaaatgtgt tttgaattgc
     2221 cacaaataat cttgccactt ctgagccatc agaaccaaga agaaagtccg cttctcgctc
     2281 caggtggatt tgccaatcct tttctggctg ctttgtgcaa gagcagactg tggatgcata
     2341 gttgtaccat cacacaaagc tttacattct aatcataaaa gataaggata tctctg
//
LOCUS       DAZUCE010000383         2378 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZUCE010000383 DAZUCE010000000
VERSION     DAZUCE010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.385"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..303
                     /locus_tag="ACHY03_005049"
     CDS             <1..303
                     /locus_tag="ACHY03_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3908146.1"
                     /translation="PDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALE
                     AGLLVTALDGLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYHL"
     gene            392..625
                     /locus_tag="ACHY03_005050"
     CDS             392..625
                     /locus_tag="ACHY03_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908147.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(645..854)
                     /locus_tag="ACHY03_005051"
     CDS             complement(645..854)
                     /locus_tag="ACHY03_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFS3908148.1"
                     /translation="MSEDIPLPKINQRYRDDHGALVTVTSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPEPRKEPPAGNHTA"
     gene            1352..1849
                     /gene="tssB"
                     /locus_tag="ACHY03_005052"
     CDS             1352..1849
                     /gene="tssB"
                     /locus_tag="ACHY03_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFS3908149.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLTFRNMNDFT
                     PEQVARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRDPELSQALRDELSAL
                     VPEQV"
     gene            1870..>2378
                     /locus_tag="ACHY03_005053"
     CDS             1870..>2378
                     /locus_tag="ACHY03_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFS3908150.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVA"
BASE COUNT          600 a          513 c          643 g          622 t
ORIGIN      
        1 ccggatacat accggtatca tctgtctcat cgtattcgcc gggtgattgg gaatgaaaac
       61 acatcatcgc cataccagca gattgcccgg gaagtgaaag cgccccgtga acggctgaag
      121 catgcgctgg aggcggggtt gctggtgact gcactggacg gcctgttctg gttcggcagt
      181 cagcgtattg cggctgatgt cctgaggctg agaaagagcg gaatgccggt ggtgacaacg
      241 accgcggagg tgcatgataa tctgacggga acaacccgca aagtaccggc ctaccatctc
      301 tgattcgacc atcaggggga atattgctct ggcagcagca gtacgacatt ctttatcccc
      361 gagcctgaaa acatgtgcta aggaaacggg aatggcaagg tccgattacg atattatcaa
      421 tctgtctccg gaacatgaac tgaatgagtg gctggcagag agaggttatg ccggacaggc
      481 ggataaccgt aaccgggtgg cagaaattgt tacccgcaaa ttgcaggaca gcttttatat
      541 caacgtttct tgggatgcgc tgaacacggc atacagtgaa cacccggagt ggttttctgg
      601 gcttgtctcc ggggatgaga attaataagt ggattatgct gccatcaggc agtgtgattg
      661 ccggcagggg gttctttccg gggttcgggt ttgaattttc ccaggaagtt gtacatcggc
      721 cgcatgcagg gatgcggata gccgtcccgc ataaacacca cccgggtttc ctcaacactg
      781 gtcacggtga caagcgcgcc atgatcgtct ctgtatcgct gattgatttt tggtaatgga
      841 atatcttctg acatagactt cagaacggta accgttcact aaattttcat gtggtatcac
      901 tgaatcagga gacgacgttt cctgatgtgc gttgttaatt ttctgttttt aaaaatattt
      961 tctctctctt ctttattttc cgtgtctttt attgctctcc tctttcatgt ccttctcttt
     1021 ttctgatatg cccttaaatg ctgccgaatc atcactcctc gtgaatgtca gtgctggtaa
     1081 tcattcggta taaccatctg tatcaaaagc actaaccgga ataattttct gcatttttgt
     1141 attacccctg gatataagtt ttgataaata ctgattttgc gatatgtgtc agaataaaga
     1201 tctggttatt ggcattaaaa cgatcgatat gtctgtcata ttgagaattt gcagatcttc
     1261 gtcctataat gggtgaaatt aaatcagtgc acaaggggag gccttctgtg gcgatgggct
     1321 ccctgggatg caggtttcac aggagaacgt catgagcagt tcgtttcaga atgagatccc
     1381 gaaggcaagg gtgaatctga aattagacct acatacaggt ggtgggcaga agaaagtgga
     1441 gcttccgctc aaactgctgg ttgccggtga tttcagtaac ggacaggaag agcgcccgct
     1501 gtcagagcgc ataaaaatgg atgttaataa aaataacttt aatgctgttc tggctgatat
     1561 ctcgccgtca gtacgcctga atgtggagaa cacacttgcc ggtgatggta ctgaagagag
     1621 catcagcctg actttccgta atatgaacga tttcacgccg gagcaggttg cccgacaaat
     1681 tccccgtctt aaagccatgc tggccatgcg cagccttctg cgggatctga aggcgaatct
     1741 gctggataac gccactttcc gtaaagaact ggaaaaaatt ctgcgggatc ccgaactgag
     1801 tcaggcctta cgtgacgaac tgagtgcact ggtgccggaa caggtgtgat taacggatta
     1861 agagagagca tgctgatgtc tgtacagaaa gaaaataatg tggcgggaaa tgcggtttct
     1921 gaaacgcatt ccggcgacag cgtatatgct tccctgtttg aaaaaattaa tctgaatccg
     1981 gtatcagccc tgagtgcgct ggatatctgg caggatccgc aggccatgtc agaggcatca
     2041 gcagatgaac gtctgaccgc agggatgcag gtgtttatgg aatgcctggc gaaagcggga
     2101 gcaccggtgg agaaactcga taaggcgctg attgaccatc atattgcaga gcttgattat
     2161 cagatcagcc gtcagctgga tgccgtgctt catcatccgg aatttcagaa agtggagtcc
     2221 ctgtggcggg gcgtgaagtc tctggtggat aaaacggact tccgccgtaa tgtgaaaatt
     2281 gaactgctgg atctgtccaa agacgatctg cgtcaggatt ttgaggatgc gccggaaatt
     2341 attcagagtg gtctttatct gcagacttat gtggcgga
//
LOCUS       DAZUCE010000384         2369 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZUCE010000384 DAZUCE010000000
VERSION     DAZUCE010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.386"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..479
                     /locus_tag="ACHY03_005054"
     CDS             <1..479
                     /locus_tag="ACHY03_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFS3908151.1"
                     /translation="DSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAV
                     SFRSIIAEQKKEPKMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDC
                     KIRRGNAVELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            489..1904
                     /locus_tag="ACHY03_005055"
                     /pseudo
     CDS             489..1904
                     /locus_tag="ACHY03_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1908..2348
                     /locus_tag="ACHY03_005056"
     CDS             1908..2348
                     /locus_tag="ACHY03_005056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908152.1"
                     /translation="MIIGNGPVPDNPFGHAAAANRYGLMSSGTGDEMGASVSDYFKKI
                     QSRRDTWIWIIDTTDEEEQCMMNKAIDLNKKLKTINLGPLPVLNNCFSRTNMIFEACG
                     FNNPSMNTNAPISLQVLGELYGYQRYIMPKGPFTGFPFEFQEVK"
BASE COUNT          629 a          564 c          716 g          460 t
ORIGIN      
        1 atgacagtta cgcaatcagt gaaaagagtc acggcagaga agaaatccgt cttcatattg
       61 tttgcgatgt ccctgatgaa cttattgatt tcacgtttga atggaaaggg ctgaagaaat
      121 tatgcgtggc agtctccttt cggtccataa tagcagaaca aaagaaagag ccaaaaatga
      181 cggtcagata ttatatcagt tctgctgatt taaccgcaga gaagttcgcc acagcaatcc
      241 gaaaccactg gcacgtggag aataagctgc actggcgtct ggacgtggta atgaatgaag
      301 acgactgcaa aataagaaga ggaaatgcag tagaattatt ttcagggata cggcacattg
      361 ctattaatat tttgacgaat gataaggtat tcaaggcagg gttaagacgt aagatgcgaa
      421 aagcagccat ggacagaaac tatctggcgt cagtccttgc ggggagcggg ctttcgtaat
      481 cttgccctgg gctggcgacc cgtcaggagc cggacggtct gccgccggtg gagtggctga
      541 cgtatggcag cggttatctt gcggggatga agctgggcgg aacgccgctg gtcgagtaca
      601 cgcgggaccg gctgcaccgt gagatggccc gcagcttcgg cggggaggca tatgaacttg
      661 ccaccgcctg gaataccagc ggccagctcc agagcaggca cctgaacctt ccgcagcttg
      721 accgtgacta cgactggaac gacaacggac agctaatccg catcagcggc ccgcaggaga
      781 gccgggagta ccgttacagt gacacgggaa ggctgacggg cgtccacacc acggcggcga
      841 acctggatat cgatatcccg tatgcaacag acccggcggg aaaccggctg ccggacccgg
      901 aactgcaccc ggacagtacc ttcacggcgt ggccggataa ccgcatcgcg gaagatgcgc
      961 actatgtcta tcgctacgat gaatacggca ggctggcgga gaagacggac cgtatcccgg
     1021 aaggggttat ccggatgcac gacgagcgca cccaccacta tcactacgac agccagcacc
     1081 ggctggtgtt ctacacgcgg atacagcatg gcgagccact ggtcgagagc cgctacctct
     1141 acgacccgct gggacggcga atggcgaaac gggtgtggcg tcgcgggcgt gacctgacgg
     1201 gatggatgtc gctgtcgcgt aaaccggagg agacatggta cgggtgggac ggcgacaggc
     1261 tgacgacggt gcagactgac accacacgta tccagacggt atacgagccg ggaagcttca
     1321 cgccgctcat ccgggtcgag acagagaacg gcgagcggga gaaagcgcag cgccgcagcc
     1381 tggcggagaa gatgcagcag gaagggagcg aggacgggca cggcgtggta tttccggctg
     1441 aactggtgcg gctgctggac agactggagg aagaaatccg ggcagaccgc gtgagcagtg
     1501 caagccgggc gtggcttgcg cagtgcgggc tgacggtgga gcaactggcc agacaggtgg
     1561 agccggaata cacaccggcg cgaaaagttc atttttacca ctgcgaccac cggggcctgc
     1621 cgctggcgct catcagcgaa gacggcaata ctgcgtgaag cggggagtat gatgagtggg
     1681 gaaacctgct gaatgaggaa aacccgcatc acctgcacca gccgtaccgt ctgccagggc
     1741 agcaacacga tgaggagtcg gggctgtatt ataaccgtca ccggtactac gatccgttgc
     1801 aggggcggta tatcatccag gacccgattg ggctgaaggg gggatggaat ctgtaccaat
     1861 atccgctgaa tccagtgcaa cacgttgatc cattagggct tagtactatg ataataggta
     1921 acggccccgt tcctgataat ccttttgggc atgctgcggc ggcaaatcga tatggattaa
     1981 tgagttcggg aactggggat gagatgggag cctctgtctc tgattatttt aaaaagatac
     2041 aatcccgcag agatacctgg atttggataa tagatacaac agatgaagaa gaacaatgta
     2101 tgatgaataa ggcaatagac cttaataaaa aactaaaaac tattaacctc gggcctcttc
     2161 ctgttttaaa taattgtttt agccgaacga acatgatttt tgaggcgtgc gggtttaaca
     2221 acccctctat gaatacaaat gcgcctataa gcctacaagt gctcggtgag ctttatggat
     2281 atcaaagata tattatgcct aaaggtcctt tcactggatt cccatttgaa tttcaagagg
     2341 taaagtaaat ggaaatgctt gggaagata
//
LOCUS       DAZUCE010000385         2337 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZUCE010000385 DAZUCE010000000
VERSION     DAZUCE010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.387"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(458..892)
                     /gene="uspF"
                     /locus_tag="ACHY03_005057"
     CDS             complement(458..892)
                     /gene="uspF"
                     /locus_tag="ACHY03_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFS3908153.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1033..2166)
                     /gene="ompN"
                     /locus_tag="ACHY03_005058"
     CDS             complement(1033..2166)
                     /gene="ompN"
                     /locus_tag="ACHY03_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFS3908154.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNDQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          656 a          494 c          540 g          647 t
ORIGIN      
        1 cctgctggcg acgtggtact gaggaaaaaa gtagggcacc tattaggctt tgaaatgact
       61 tgatgaggaa aattgttgat ggcaaaaatg gggcaaagcg ctgcaaaagg ggcaaaaaag
      121 gggcaaaaaa agagtgggtt atcgtagctt attgttgtcg ctgatgatat ttaacacatt
      181 gaaaaataag taaaatactt atgagtcaga gagttgtgat ttttgccctt acttgttcag
      241 gttgtattgt tctttcttgc taatttcttg attttgcgac atttaaaagc gactcaattc
      301 gttatatggc atcagaagag tatgcgtcat gccggaatgc ccagcataag aaatctgata
      361 taaaaacagt ggcgtgtatg gtacggatta gaggggaaaa tgtcagcaca tttgcgaaat
      421 gaatcaaaaa gcccgcagca atgtgcgggc gttagtgtca gcgcacaacc agcacggagc
      481 actctgcgtg acgcacgaca gctgcggcgt tagaaccgag cagataagtg gtgatatccg
      541 gtcgatggga agcaatgatg atcatatgag cggggatctt cttcgccaat tccagaatgc
      601 ggtctttggg cgaaccttcc tcaacatgga catgcactct gtcggttggc agtttaaatt
      661 ttttaatgat ctcttccagt tgcgatttgg cttccgcttt caggtcatcc attgccggta
      721 attctgcgga atacgctaaa cccagagagg catagtaggg cagtgaaggt attaccgtca
      781 ggaaatgaac ctctgcatca tcaatctttg cctctgcctc aacgtggcta atcacgcgtt
      841 gagttaattc tgaatcggaa atatcgatag ggacaagaat cgttctgttc ataaaacctc
      901 ctgttttagt atccgcataa agtgtaacgc cagatgacac tttttgtgta atgacggagt
      961 tcacattttt aatttagatc aaaggaggaa gaataagcag aaaaagcccg ccataacagc
     1021 gggcaggagg atttagaact gataaaccag acctaaagcg acaatatcat cggtagagat
     1081 gccgttggca gcgtagaaac tgtcatcttc atccaacagg ttgattttat agtcaacata
     1141 agtggacatg tttttattga aatagtaagt cgcgccaaca tcggcgtatt taaccagatc
     1201 tttatcatca acacctgccg ggttgtctgc accaccggca gcgtgcaggt cacggccttt
     1261 agacatcagg aaagagactg ccggacgcag accaaaatca aactggtact gtgcagtgac
     1321 ttcaaagttc tgtgttttgt ttgcaacagc ataatcgctg tcgccaaacg gggtcatatt
     1381 acgcgtttct gaatacatgg ttgccaggta aatattgtta gcatcgtatt ttagcccagc
     1441 agtccacgcg tctgctttat caccacccgc cgcagtatgg ttaacctggt cattggtgcg
     1501 gtcagaagag gtgtatgccg caccagcgct aaagcccatg cctaaatcat atgttgtgga
     1561 aagaccccag ccgtcaccgt tttcatggcg aacatcacgt ccgttgttgg tgccttcctg
     1621 atcattactg gctccttcgt tgttaccttg atactgcacc gcgaagttca gaccatttac
     1681 cagaccgaag aaatcagtat tacgataagt cgcgacgcca ttggctcgac cagtcataaa
     1741 gttgtctgca ttggtataag agtcaccgcc aaattcaggc agcatatcgg tccagccttc
     1801 gatgtcgtac attacgccat aattacgtcc gtaatcgaaa gaaccgtaat ctgcaaattt
     1861 cagtccggca aatgccagac gggtccatga ctggtttttt gaagattcag tgttgtttgc
     1921 ctgaatattg tattcccatt gaccgtagcc agtgagttga tcgttaattt gggtttcgcc
     1981 tttaaaaccc agacgcgcat agctctggtc gccatctttc gctgaattat cagaaaaata
     2041 atgcaggcca tcaactttgc catacagatc taatttgttg ccgtctttat tataaacttc
     2101 ggctgcatgt gcagcacctg cggcgagcag ggcaggaatt aaaagtgcca gtactttgct
     2161 tttcattgaa taaatccttt agttatttta tttgcctttt atccccattt cggggagtga
     2221 aaacatccta aaggagaagt tcagttagcc gataaatatc atttgttacg ccttaagtaa
     2281 aaccttaatc aaataattcc tctgaaatga taataagtct ggtgaatgta tcgaaga
//
LOCUS       DAZUCE010000386         2307 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZUCE010000386 DAZUCE010000000
VERSION     DAZUCE010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.388"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..269)
                     /locus_tag="ACHY03_005059"
     CDS             complement(<1..269)
                     /locus_tag="ACHY03_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFS3908155.1"
                     /translation="MSTDKEEFSLYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     VVAMDEAGYDADDFKKPVRVHFPVVNDLPPEGVFDTEFCNRYEKGG"
     gene            complement(362..550)
                     /locus_tag="ACHY03_005060"
     CDS             complement(362..550)
                     /locus_tag="ACHY03_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFS3908156.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCLRARDEQKISGE
                     CLPLKKVSLYLNNEIPAG"
     gene            complement(547..735)
                     /gene="dicB"
                     /locus_tag="ACHY03_005061"
     CDS             complement(547..735)
                     /gene="dicB"
                     /locus_tag="ACHY03_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFS3908157.1"
                     /translation="METLLPNVNTSEGCFDIGILLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            complement(746..1597)
                     /locus_tag="ACHY03_005062"
     CDS             complement(746..1597)
                     /locus_tag="ACHY03_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS3908158.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGIATPMFIMAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPQGWPGSMMTGCSNPVRLTTHEIATSGGELMKFIMEAAIMATI
                     PALSHPEITIANGRAVTSSLAVAEYFRKPHKDVLAKISRLDCSAEFTERNFSPSEYTD
                     STGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKMI
                     HGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTVE
                     QLLKIHC"
     gene            complement(2116..2268)
                     /locus_tag="ACHY03_005063"
     CDS             complement(2116..2268)
                     /locus_tag="ACHY03_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFS3908159.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
BASE COUNT          593 a          551 c          533 g          630 t
ORIGIN      
        1 ccgccttttt catagcggtt gcagaattcg gtatcaaaca cgccttccgg tggaaggtca
       61 ttcacaacgg ggaaatggac gcggacaggt tttttgaaat catcagcgtc gtagcctgcc
      121 tcgtccattg caacaacgca ccgtgagatg gcaacagata atttttttgc tgtagtccag
      181 taaaaacctc ctttaatccc aaggcgtttt ctgactttgt cattttttgc ttcgcaatat
      241 agcgaaaatt cttctttatc agtgctcatg gataaacctc ataactattt taaggttgta
      301 cgaacccctg ccattgctgg catgcttaat caacgggtat ggcgttaata tggctggtgg
      361 attatccagc cggtatttcg ttattcaggt acagcgatac tttttttaac gggaggcatt
      421 cgccagaaat tttttgctcg tctcttgcac ggaggcatga ttctttactg gcataaattc
      481 cggtaatcac attctgtggc tcacccgtta taagaaaaac cgtcatcacc agtgcaaatg
      541 cagaagtcac tgctgccctc cgaaaatacc aagtttaaga agggcaattc tggagagtat
      601 ggaattatca ttgagaagat aaggctcata ttttctcatc ttaatggcat cttcagtaaa
      661 ctcccggtta ctgagcagaa taccaatatc aaaacaccct tcagacgtat taacgtttgg
      721 taataacgtt tccattatcg cgtcctcaac aatgaatttt tagtaattgc tccacagtca
      781 tatttttaat tgcgctccgg tttacaagag tccagccttg tttctccaga taaaactgga
      841 aggtatccag ggtgcagacc attgcaccat caggaacggt ttcagtgaat ttgatattgc
      901 cgtgttcgtc gagacggata accagggtac gtccgtcccc gtgaatcatt ttgtcgggag
      961 acggggcgtt attctggcgc agttctttct ccatgcgatc gaactcagcg atgtaggctt
     1021 ctttaaatgc agcggctttt ttgccggtga agcccatcac caggaaaacg aagccgtttt
     1081 tggtgatttg gtacataggg agtttgcgcc cggttgagtc ggtatattca ctgggcgaaa
     1141 aatttcgctc agtgaattct gctgaacagt ctagacgtga aatttttgcc agtacatctt
     1201 tgtgtggttt ccggaaatac tcggcaacag ctaaagagga tgtaacagca cgcccattgg
     1261 cgatagtaat ttcagggtga gaaagggcag ggatagtagc catgatggca gcctccatga
     1321 tgaatttcat taactcacca ccggaggtag caatctcatg ggtggtgaga cgcacagggt
     1381 tgctacaacc ggtcatcatg gaacccggcc agccttgcgg ctgccctgca cgccccacca
     1441 taatctgaat gtggctgtgc attacgcata aaaaaaccgc ctgagcgcgg ttatgcgcca
     1501 tgatgaacat cggggtagca atcccggcac ccgctttatg aggtgcaggt gcactataat
     1561 tccacccgtt ctggttttca atagctacat tcaacatttt ctcttacctt tcatcaccga
     1621 agtgaacttt gtcgatgcag tgcctggtgc ctccaggtga cgttaaccag ttaacaatta
     1681 acgccggaat acagaaggat gcccgttacg ccccgtaaaa gaccacttta cggttttaac
     1741 tgttccgcgt gcgcttagcc gcattcaccg catcacaaaa ttcactttaa aaagggcgga
     1801 catcagaaag ctaataaact gatgccgcca aaggtaccac tgccattgaa acatggagat
     1861 gttgtggcgg ggttgtcact caggcatatg gtcaacctga caacccggtg tcctcaatga
     1921 ggggaaagga aataaaaacc cccagccata tttaccgccg cgccatttcg cggattgcca
     1981 caaccggaag cacacggtcg aagaaatcta acgacaagcc ttctaaggaa aagacgcctt
     2041 cgccgtgtgc tttctgttgt gccctgactt ttcagggtaa attaaccagt ggcaacctgt
     2101 ttttactggt gctaatcagt tagtgtttct ggctaaccag cgacgcgcgc cagcttcggt
     2161 tttaaacttt ttacttttgg tatacgtcat cgcggtaaac gtgccgtcct ggttagggaa
     2221 cacaccgcat accagagatt cgttgttgcc aagattgagc gtatccatgt tgacctcatt
     2281 tccccttaac gccggggtag cggaaca
//
LOCUS       DAZUCE010000387         2303 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZUCE010000387 DAZUCE010000000
VERSION     DAZUCE010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.389"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..678
                     /locus_tag="ACHY03_005064"
     CDS             <1..678
                     /locus_tag="ACHY03_005064"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFS3908160.1"
                     /translation="PPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGNVG
                     RAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRG
                     LIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLN
                     VDLDVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLIDAEKAITDF
                     MTARGYH"
     gene            complement(726..2225)
                     /gene="pitB"
                     /locus_tag="ACHY03_005065"
     CDS             complement(726..2225)
                     /gene="pitB"
                     /locus_tag="ACHY03_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFS3908161.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGMQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          618 a          597 c          563 g          525 t
ORIGIN      
        1 cctccattaa ttgccgttat tggtagcgat ggttcaggca agtcaacggt gtgtgaacat
       61 ctcattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg aaaacaagcc
      121 ggaaatgtcg gtcgtgcagt gacaaaatta ccgttgatgg gaaaatcctt acataaaaca
      181 attgaacgaa atcaggtgaa aacagcaaaa aaattgccag gaccagttcc ggcgctggta
      241 attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt
      301 cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg cgcttacgat
      361 ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct ggcaagccaa
      421 gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat caaactcaat
      481 gtcgatctag acgtggcctg cgcgcgtaag cctgaccaca aaagggaatc gctggcaaga
      541 aagatagcca taacgccaca gttaaccttt ggtggtgcac aactggttga tatcgacgcc
      601 aatcagccac tggaacaggt gctgattgat gcagaaaaag cgattacgga ttttatgacc
      661 gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg ggcatttcag
      721 gaaggttaaa tcaactgcaa tgctatccag tacagcccac cagaaagaaa aattgccgcc
      781 ggtaaagtaa acacccatgc catcaggatg ctggttaccg ttttacgctg taacccaccg
      841 ccgtccacca ccatcgtccc tgcaactgca gacgagagga cgtgtgttgt ggagacgggc
      901 atcccaatat aactggcaag accgatagac actgccgccg tcatttgtgc cgccatgcct
      961 tgcgcatacg tcatgccgcg cttaccaatc ttctcaccga tggtcatcgc tacacgacgc
     1021 cagccaatca tggtgccaat gccgagcgcc agtgctaccg ccatgatgat ccacaccgga
     1081 gcgtactcaa tggtgcttaa catatcgctg cgaagttttt tcagcaggtt ctggtcttct
     1141 ttactgacgc ctggcagttt cgccagcttc gcggaggtat cagagatgca cagcataatg
     1201 cggcgcagct ggctgcgctg actcacgctt aacggctcgt aactttccat attgcctggc
     1261 agcatcgttt taacgcgcgc aatcgcatca aaggtatttg ccggatgaca gtgaaactcc
     1321 gttacttgca tgccatcagt cgatgccgca ggcaatggag gttccatcgc aatcaacttc
     1381 tgcggcagtt caggatgctg ttgcaggtag tgttcgaagt tggtgacggc atcgcgggta
     1441 cgggtaattt catagccgga cgcgttcata ttgacgacga agccagcagg ggcaatcccc
     1501 accagtacca gcatcaccag gccgatccct ttctgtccgt cgttcgcgcc gtgcgaaaac
     1561 gccacgcccg cagcggaaac aatcagcgca atacgcgtcc agaatggcgg cttacgtttg
     1621 ccttttttct ttttgcgatc ttccggaatg cggtgaatac ggtcacgctt tttcgtcccg
     1681 ctccagtagc gtcgcagcag gaatatcagg cctcccgcaa tgaccaggcc gacgataggg
     1741 gaaacaatca gcgaggagaa aattttggtc acttcacgca ggtttaacgc atccatcacc
     1801 gatgagccgg ttaacagcgc gttggttaaa ccgatgccga taatcgcgcc aatcaaggta
     1861 tgcgaactgg aggccggtaa accaaagaac cacgttccca agttccagat aatcgccgct
     1921 agcagcatgg aaaagaccat cgccagaccg tgggttgacc ccatattcag caacaaatcg
     1981 gttggtaaca tatggacaat ggcataggca acgctaagtc cgcccaataa cacgccaaaa
     2041 aagttaaaaa atgccgccat caccacagca agttgtggtt gcatggcacg agtataaata
     2101 acggttgcca ccgcattcgc cgtgtcatga aaaccattga ttgcttcgta gaacaacaca
     2161 aatgccagag caagtaataa caaaagccct gtgtatatat caaggccaac aaataaattt
     2221 agcatatatt agattacgcc attttgaatt tacgaacgga cgcattatca gtgactttaa
     2281 cggtgtgggc aaagtgaaat atc
//
LOCUS       DAZUCE010000388         2281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZUCE010000388 DAZUCE010000000
VERSION     DAZUCE010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.390"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..185
                     /locus_tag="ACHY03_005066"
     CDS             <1..185
                     /locus_tag="ACHY03_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908162.1"
                     /translation="RIGRCMGYLQVAAEIEALASGADKDAVVRETVLRDFNTPPFKTE
                     PDDWIQPGLNYLKGRI"
     gene            complement(197..511)
                     /locus_tag="ACHY03_005067"
     CDS             complement(197..511)
                     /locus_tag="ACHY03_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908163.1"
                     /translation="MHNHQHNYDLCLQAINERVKSECLLLLPQEHKAVKAIQAEPYGA
                     LTTATLSIISGALTPTLLLRLKDNIDDWLNEELSYLDCEWDNHYAKSQKDRLFRQLSG
                     NR"
     gene            640..1173
                     /locus_tag="ACHY03_005068"
     CDS             640..1173
                     /locus_tag="ACHY03_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS3908164.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATQVDGKPVIPGMKLSKEKCDQVNAIERDKALTWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1394..1507
                     /locus_tag="ACHY03_005069"
     CDS             1394..1507
                     /locus_tag="ACHY03_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908165.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIIAIAVLIWAIRWW"
     gene            1509..1976
                     /locus_tag="ACHY03_005070"
     CDS             1509..1976
                     /locus_tag="ACHY03_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFS3908166.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENEILRADVAAGRKRLRINATCPGP
                     VREATGTARVDNATGPQLADTVTRDYFTLRERLMTMQKQLEGAQEYIRTQCIK"
     gene            2000..2224
                     /locus_tag="ACHY03_005071"
     CDS             2000..2224
                     /locus_tag="ACHY03_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908167.1"
                     /translation="MKKLLVTVKPFQGTIPFRVLQPGRVLVEGTFSGKCTQSYSRVFR
                     VDASNEELTVECAVNAAKCRMVSAALQPVC"
BASE COUNT          570 a          502 c          632 g          577 t
ORIGIN      
        1 agcgtattgg tcgttgcatg ggatatcttc aggtagctgc tgagattgaa gcgctggcca
       61 gtggtgctga caaggatgca gttgtgcggg agaccgttct tcgtgatttc aatactccac
      121 cctttaagac tgagccagat gattggatcc agccggggct gaattatctc aaagggcgga
      181 tataagttca ggggcgttat ctgttacctg ataattgtct gaacaggcga tctttttgtg
      241 atttggcgta atgattatcc cattcgcaat caaggtaact taattcttca tttaaccagt
      301 catctatatt gtctttcagg cgaaggagca aggttggcgt taatgctccg gatataatac
      361 tgagagttgc agtagtgaga gctccatacg gttcagcctg aatggccttt actgctttgt
      421 gttcttgcgg gaggagtaag aggcattccg attttacccg ctcatttatg gcttgcaggc
      481 ataaatcata attatgttga tggttatgca tggttaatcc tcgactggaa ttgtcagata
      541 tattttcagc cagcagataa aacgccagtg cccaccactg gcgggctgaa gacttaacat
      601 atccagggat tcggaaccga taaatcctga taaatatcca tgaacgcaaa aatcagatac
      661 ggcctgtcgg ctgccgttct ggcgctgatt gccgcagggg cgcctgcgcc tgatattctc
      721 gaccagtttc ttgacgaaaa agaaggcaac cacaccacag cataccgtga tggtgcgggt
      781 atctggacca tctgccgagg tgccacccag gtggatggta agcctgtgat tcctggcatg
      841 aagctgtcga aggaaaaatg cgaccaggtt aacgccattg agcgtgataa agcgctgacg
      901 tgggtggaga aaaacatcag agtgccgctg accgaacccc agaaagcggg gatcgcgtca
      961 ttctgtccgt acaacattgg tcccggtaag tgtttcccgt cgacgtttta cagacgaatt
     1021 aatgcaggtg atcgcagggg agcatgcgaa gcgattcgct ggtggattaa ggacggtggc
     1081 agagactgcc gtattcgctc aaataactgt tacggtcagg tatcccgtcg tgaccaggag
     1141 agcgcgctgg cgtgctgggg aatcgacaga taagcagaat attttgctga aaaatgacgt
     1201 tgaccaacgc ggatggataa cacgaaatcc tgcgaactgg caaaacctaa gtgaataaaa
     1261 gtaaaaaccc cgtttgttgg cagcaagcgg ggttttgtgt ttctgacctt ggataaggca
     1321 agggagaaat tatggtgagg gaggtgcttt ccctgtgagg aagtataaaa gattctttct
     1381 gaggttgtcc attatgaaag gcattgaagt ggagacgcca gccagtctgg atttaacaag
     1441 agcggcagct tttgccattc gtattatagc cattgctgtt ctgatctggg caatccgttg
     1501 gtggtgaaat gaaccgcgtt ctgtgtgtgg tcatcattgc tctgctggtg gcctgtggtg
     1561 cgcttagtct ggggctgaat cattaccgcg ataacgccat cacttacaaa gcgcagcgcg
     1621 ataaaaaagc cagagagctg gagctggcaa acgcaaccat tactgatatg caggtgcgcc
     1681 agcgtgatgt tgctgcgctc gatgcaaaat actcgaggga attagccgat gcgagagctg
     1741 aaaatgaaat tcttcgcgct gacgttgccg ctggtcgtaa gcgcctgcgt atcaacgcca
     1801 cctgtccagg tcccgtgcgt gaagccaccg gcaccgcccg cgtggataat gcaaccggcc
     1861 cccaactggc agacaccgtt acacgggatt atttcaccct cagagagcgg ctgatgacga
     1921 tgcagaagca actggaaggg gcgcaggaat atatccgtac tcagtgcatt aagtagtctt
     1981 tttattatcc ggaggatata tgaagaaatt actggtaacc gtaaagcctt ttcagggaac
     2041 aattccgttc cgtgttttgc agcctggtcg tgttcttgtt gaaggtacgt tcagtggtaa
     2101 atgtacgcaa tcatactccc gggtctttcg ggtggatgcc tcgaatgaag agctaaccgt
     2161 ggagtgtgct gtgaatgccg ctaaatgccg catggtatcg gctgcattac agccagtgtg
     2221 ttgagcgacc ttattatcca tggcgcggta ttgtcgccgt attcccgtat taacagagac
     2281 c
//
LOCUS       DAZUCE010000389         2263 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZUCE010000389 DAZUCE010000000
VERSION     DAZUCE010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.391"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..264)
                     /locus_tag="ACHY03_005072"
     CDS             complement(<1..264)
                     /locus_tag="ACHY03_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFS3908168.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINSNSQRVLQSQPGEL"
     gene            386..2131
                     /gene="ggt"
                     /locus_tag="ACHY03_005073"
     CDS             386..2131
                     /gene="ggt"
                     /locus_tag="ACHY03_005073"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFS3908169.1"
                     /translation="MIKPTFLRRAAIATLLSGCCFSAAAAPPAPPPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALE
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT          549 a          547 c          647 g          520 t
ORIGIN      
        1 tagctccccc ggctgcgact gcaatacccg ctgagagttg ctattaatct gattttctaa
       61 atgctgctgt tgtaattgcg tctgcgtttt cagttgctga ttcagcatcc ctttttgctg
      121 gatttgctga gtctgcatct gggtttgcat ccgctgctgg ctggggatct gatatcccgg
      181 ctggttgggg ttattcagag tattaatggg ctgtgcaaag ccgacaaacg gcaggagtgc
      241 cgtcaaaagc agaagtcgtt tcatcgcgta tcctcctctg aagatatcct ttaagtttac
      301 tctgttcccg gcgaaacgat gattatttca gagttataca ccaggcttaa gtggggaagg
      361 cccctcacat aactggagaa taacgatgat aaaaccgacg tttttacgcc gggcggcgat
      421 tgctactttg ctttcaggat gctgttttag tgccgccgcc gcgccacctg cgccgccgcc
      481 cgtttcgtat ggcgtggagg aagatgtctt ccatccggta cgcgcgaagc agggaatggt
      541 agcgtctgtg gacgccactg ccacccaggt gggggtagat attctaaagg agggcgggaa
      601 tgccgttgat gccgctgtgg cagtggggta tgcgctggcg gtaacacatc cgcaggcagg
      661 gaatctgggc ggcggtggtt ttatgctgat ccgatcgaaa aatggcaata ccaccgctat
      721 cgatttccgc gaaatggcgc ccgccaaagc aacacgcgat atgttcctcg atgaccaggg
      781 caacccggac agcaaaaaat cactcacttc gcatctggct tccggcacac cgggtacggt
      841 cgcgggcttc tcgttggcgc tggagaagta cggcaccatg ccgttgaata aagtggtgca
      901 accggcgttt aaactggcac gcgatggttt tatcgttaac gatgcgctgg ccgacgatct
      961 caaaacctac ggtagcgaag tgttgccgaa tcacgaaaac agcaaagcta tcttctggaa
     1021 agagggggaa ccgctgaaaa agggtgacaa gctggtgcag gcgaacctcg caaagagcct
     1081 ggagatgatt gccgaaaacg gcccggacga attctacaaa ggcacgattg cggaacagat
     1141 cgcccaggag atgcagaaaa acggtggctt gatcactaaa gaagatttag cggcctataa
     1201 agcggtcgaa cgtacaccga ttagcggcga ttatcgcggg tatcaggttt actccatgcc
     1261 accgccatct tccggcggga tccatatcgt gcagatcctc aatattctgg aaaacttcga
     1321 tatgcagaaa tacggctttg gcagtgccga tgcgatgcaa atcatggcag aggctgagaa
     1381 atacgcctac gccgaccgct cggaatatct tggcgatccg gattttgtca aagtcccgtg
     1441 gcaggcgctg accaataaag cctatgccaa atctattgcc gatcaaatcg atatcaataa
     1501 agcgaagcca tccagcgaaa ttcgccctgg caagcttgcg ccttatgaga gtaatcaaac
     1561 tacccattac tcagtggtgg ataaagacgg taacgcggta gcggtgacct atacgctgaa
     1621 caccaccttc ggtacgggga ttgtcgcggg cgagagcggt attttgctca ataaccaaat
     1681 ggatgatttc tccgccaaac cgggcgtacc gaacgtttac gggctggtgg gcggtgatgc
     1741 caacgccgtc gggccgaaca aacgcccgct gtcgtcgatg tcaccgacca ttgtggtgaa
     1801 agacggtaaa acctggctgg tgactggcag cccaggcggg agccggatta tcaccaccgt
     1861 gctgcaaatg gtggtgaata gcatcgatta tggcatgaac gtcgccgaag cgactaatgc
     1921 gccgcgtttc catcaccagt ggttgccgga cgagctgcgt gtcgagaaag gatttagccc
     1981 ggatacgctc aagctgctgg aagcaaaagg tcagaaagtg gcgctgaaag aggcgatggg
     2041 cagtacacaa agcattatgg ttgggccgga cggtgagttg tacggcgcat ccgacccgcg
     2101 ctcggtggat gatttaactg cggggtatta aggttagcgg ccctcttcgt gggaagaggg
     2161 ctgttttctt gaacaagata aattagtgtc tggattgctt ttactattgc tccgtaatat
     2221 ataatcataa ttatcctgaa tgcttttaag gggcttaggt tat
//
LOCUS       DAZUCE010000390         2261 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZUCE010000390 DAZUCE010000000
VERSION     DAZUCE010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.392"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            151..654
                     /locus_tag="ACHY03_005074"
     CDS             151..654
                     /locus_tag="ACHY03_005074"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3908170.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALKAMEEAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(700..1116)
                     /gene="rraB"
                     /locus_tag="ACHY03_005075"
     CDS             complement(700..1116)
                     /gene="rraB"
                     /locus_tag="ACHY03_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFS3908171.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1278..>2261
                     /gene="argF"
                     /locus_tag="ACHY03_005076"
     CDS             1278..>2261
                     /gene="argF"
                     /locus_tag="ACHY03_005076"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFS3908172.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVM"
BASE COUNT          581 a          529 c          569 g          582 t
ORIGIN      
        1 atacagacag taaaatgttc tgccacgtta caatactctt atcagtaata ggctggcaaa
       61 aactttattg ctttataaga tgcttgcgca cgtaagaaga gagtggtatt aaacaaatgt
      121 ataattactt tatttttatt gggttaaact atgaataaca ttgcgccgca atcacctgta
      181 atgcgtcgcc tgacgttgca ggataatcct gctatcgccc gcgtcattcg tcaggtatcc
      241 gccgaatacg gtcttaccgc tgataaaggc tacaccgtcg ccgatccgaa tcttgacgag
      301 ctgtatcaag tgtatagtca gcctggtcat gcatattggg tggttgagta cgagggtgaa
      361 gtggtcggcg gcggtgggat tgcgccatta gcaggtagtg aatcggacat ttgcgaactg
      421 caaaagatgt attttcttcc ggctattcgc ggcaaagggc tggcaaaaaa actggcctta
      481 aaggcgatgg aggaggcaag agaaatgggc ttcaaacgct gctatctgga aacgaccgct
      541 tttttgaaag aagcgattgg tttgtatgaa catttaggtt ttcaacatat cgactatgca
      601 cttggctgca cgggccatgt tgattgtgaa gtgcggatgt tgcgcgaact ctaaaaaaat
      661 gcccgctagt gtgtgctatc gggcatttta atcaattaat tagtggcgaa ccccgtcatc
      721 gtcttcatcg acaaaatctt catcgtcgcc atcttcgccg ttgggatctt caaagtaagt
      781 gccccaaccg tcgtactcaa cgtcaaattt ctccgccagc gtcatcagtt gttcaacctg
      841 ggcatcgatc agatcggcat tcaacgcgca ctcgctgagg atgtcgcagc aaatcacgat
      901 atcaccatct tcaacttcca gctcttctgg atcggtcact tcgtaaccga gtttaaacgc
      961 ttcaactgcg gctttttcca gggtttccag atcgtctgcg gaaagatggt gttcgatggt
     1021 gtacagcgcg tccggatcgc tgccatcttc cagtaattct tcaataatca aacgtgtttc
     1081 ttcacgctgt tcttccagtt gttccgggtt tgccatggct cattcctctt aatgtcctgc
     1141 cgatactctt attgtcacac accactgaca ttgcctccac ctttgtgaca aagatttatg
     1201 ctttagactt gcaaatgaat aatcatccat ataaattgaa ttttaattca ttgaggcgtt
     1261 agccacagga gggatctatg tccgggtttt atcataagca tttcctgaaa ttactcgatt
     1321 tcacgccagc tgaactcaac agcctgctgc agttagccgc gaagctgaaa gccgataaga
     1381 aaagcggcaa agaagaagcc aaactcactg gtaaaaacat cgcgctcatc ttcgaaaaag
     1441 actcgacccg tacccgatgc tctttcgaag ttgccgcata tgaccagggt gctcgcgtga
     1501 cttatctcgg cccaagcggc agtcagattg gtcataaaga gtcgattaaa gacactgccc
     1561 gcgtgctggg tcgcatgtat gacggtattc agtatcgcgg ctatggtcag gagattgtcg
     1621 aaacactggc ggaatacgct ggcgtgccgg tatggaacgg cctgaccaat gagtttcacc
     1681 ccacgcagtt gctggcggat ctcctcacca tgcaggagca tttacccggt aaagcattca
     1741 acgaaatgac gctggtctat gcaggtgacg cacgtaacaa catgggcaat tcgatgctcg
     1801 aagctgcggc gcttaccggt ctggatttgc gtctggtcgc gccacaggca tgctggccgg
     1861 aagctgcgct ggttacggaa tgccgcgctc tggcacagca aaatggcggg aatattacgc
     1921 taacggaaga tgtagccaag ggagtagaag gtgctgactt tatctatacc gatgtgtggg
     1981 tgtcgatggg ggaagcaaaa gaaaaatggg ctgaacgcat tgcattactg cgtgattatc
     2041 aggtgaacag caagatgatg cagttgaccg gtaacccgga ggtcaaattc ctccactgcc
     2101 tgcccgcgtt ccatgatgac caaacgacgc ttggtaagaa aatggcggaa gagtttggcc
     2161 tacatggcgg aatggaagtg actgatgagg tcttcgaatc tgccgccagc attgtttttg
     2221 atcaggcgga aaaccgcatg cataccatca aagcggtgat g
//
LOCUS       DAZUCE010000391         2250 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZUCE010000391 DAZUCE010000000
VERSION     DAZUCE010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.393"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(60..269)
                     /gene="fumD"
                     /locus_tag="ACHY03_005077"
     CDS             complement(60..269)
                     /gene="fumD"
                     /locus_tag="ACHY03_005077"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFS3908173.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            624..818
                     /locus_tag="ACHY03_005078"
     CDS             624..818
                     /locus_tag="ACHY03_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908174.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            826..2238
                     /gene="pykF"
                     /locus_tag="ACHY03_005079"
     CDS             826..2238
                     /gene="pykF"
                     /locus_tag="ACHY03_005079"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFS3908175.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          611 a          562 c          498 g          579 t
ORIGIN      
        1 actggaccgc aactgaagca agaatatcag gatgaagctt accgcctcat cctgaatggt
       61 cattttacca gtgcgttaat gacggtttcc atcgcctgct tttccagctc actgcgctga
      121 atgcgtttat tggctaatgc tgtacgtaat acgcctgcga taacaatatg ctttggttcc
      181 tgccccagat cgcgcatttc cagcacaact ttaccgacaa ctctgcacat ttctcggtac
      241 agttcatcct cttttgtccg atttcccatt ctttatacct atttatcatg ccaactatca
      301 gcatatatca atctaaccaa ttagacaaaa atcaaacaaa atcagacaaa taacgcgata
      361 aattatttta actgtagcaa ttgagcgatg atgtatttat acaccggatg aactttcgct
      421 tatcctcaca ctgacaaccc cggcaccaga cgttgcgcaa acagcgaagt ttttgcgtaa
      481 ccttttccct ggaacgtcaa atctttgata acaatttatt gtctaacaag ttgtatattt
      541 tttgaaacgc tgtttttgtt ttccttttgg attaatttca gcgtataatg cgcgccaatt
      601 gactcttgaa tggtttcagc actttggact gtagaactca acgactcaaa aacaggcact
      661 cacgttgggc tgagacacaa gcacacattc ctctgcacgc tttttcgatg tcacctatcc
      721 ttagagcgag gcaccaccac tttcgtaata ccggattcgc tttccggcag tgcgcccaga
      781 aagcaagttt ctcccatcct tctcaactta aagactaaga ctgtcatgaa aaagaccaaa
      841 attgtttgca ccatcggacc gaaaaccgaa tctgaagaga tgttagctaa aatgctggac
      901 gctggcatga acgttatgcg tctgaacttc tctcatggtg actatgcaga acacggtcag
      961 cgcattcaga atctgcgcaa cgtgatgagc aaaactggta aaaccgccgc tatcctgctt
     1021 gataccaaag gtccggaaat ccgcaccatg aaactggaag gcggtaacga cgtttctctg
     1081 aaagctggtc agacctttac tttcactact gataaatctg ttatcggcaa cagcgaaatg
     1141 gttgcggtaa cgtatgaagg tttcactact gacctgtctg ttggcaacac cgtactggtt
     1201 gacgatggtc tgatcggtat ggaagttact gccattgaag gtaacaaagt tatctgtaaa
     1261 gtgctgaaca acggcgacct gggtgaaaac aaaggtgtga acctgcctgg cgtttccatt
     1321 gccctgccag cactggctga aaaagacaaa caggacctga tctttggttg cgaacaaggc
     1381 gtagactttg ttgctgcttc ctttattcgt aagcgttctg acgttatcga aatccgtgag
     1441 cacctgaaag cgcacggcgg cgaaaacatc cacatcatct ccaaaatcga aaaccaggaa
     1501 ggcctcaaca acttcgacga aatcctcgaa gcctctgacg gcatcatggt tgcgcgtggc
     1561 gacctgggtg tagaaatccc ggtagaagaa gttatcttcg cccagaagat gatgatcgaa
     1621 aaatgtatcc gtgcacgtaa agtcgttatc actgcgaccc agatgctgga ttccatgatc
     1681 aaaaacccac gcccgactcg cgcagaagcc ggtgacgttg caaacgccat cctcgacggt
     1741 actgacgcag tgatgctgtc tggtgaatcc gcaaaaggta aatacccgct ggaagcggtt
     1801 tctatcatgg cgaccatctg cgaacgtacc gaccgcgtga tgaacagccg tctcgagttc
     1861 aacaatgaca atcgtaaact gcgcattacc gaagcggtat gccgtggtgc cgttgaaact
     1921 gctgaaaaac tggatgctcc gctgatcgtg gttgctaccc agggcggtaa atctgctcgc
     1981 gcagtacgta aatacttccc ggatgccacc atcctggcac tgaccaccaa cgaaaaaacg
     2041 gctcatcagt tggtactgag caaaggcgtt gtgccgcagc tggttaaaga gatcacttct
     2101 actgatgatt tctaccgtct gggtaaagaa ctggctctgc agagcggtct ggcacacaaa
     2161 ggtgacgttg tagttatggt ttctggtgca ctggtaccga gcggcactac taacaccgca
     2221 tctgttcacg tcctgtaata ttgcttttgt
//
LOCUS       DAZUCE010000392         2246 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZUCE010000392 DAZUCE010000000
VERSION     DAZUCE010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.394"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            448..741
                     /gene="iss"
                     /locus_tag="ACHY03_005080"
     CDS             448..741
                     /gene="iss"
                     /locus_tag="ACHY03_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFS3908176.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(832..1014)
                     /gene="rzoD"
                     /locus_tag="ACHY03_005081"
     CDS             complement(832..1014)
                     /gene="rzoD"
                     /locus_tag="ACHY03_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFS3908177.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1231..1728)
                     /gene="rrrD"
                     /locus_tag="ACHY03_005082"
     CDS             complement(1231..1728)
                     /gene="rrrD"
                     /locus_tag="ACHY03_005082"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFS3908178.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWEGLMTRREIEREIC
                     LWGQQ"
     gene            complement(1728..1943)
                     /gene="essD"
                     /locus_tag="ACHY03_005083"
     CDS             complement(1728..1943)
                     /gene="essD"
                     /locus_tag="ACHY03_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFS3908179.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          607 a          548 c          495 g          596 t
ORIGIN      
        1 tggccgttct ggtctacttc gtaatgggat ttaatagctg aacgacaaaa gtcttgcgac
       61 cacagtcaca cagacctgaa tacacgtcct gtttcttcca cccccgcaca ggactggcga
      121 gcatgaggga cacccccgcg aaccataaac gcggtaaaaa cccggtgtgc atcgtttttg
      181 attattcccg cacactcacg cagaaggaat tccccgtcgg gctacggtca tggttaatgc
      241 gggaatacgg cgacgataca gcgctgctaa aagggtaatg aacagataga gcggtttatt
      301 tcattccaca ggattctgag tgcccccccc ctcctccaat aggctgagca tccacctata
      361 tagttttaat tttcatcaat ccatttaact atcgtttaat tgttgtcaca taggattctg
      421 ccgtttttaa caatgcagga taataagatg aaaaaaatgt tgttttctgc cgctctggca
      481 atgcttatta caggatgtgc tcaacagacg tttactgttg gaaacaaacc gacagcagta
      541 acaccaaagg aaaccatcac ccatcatttc ttcgtttcgg gaattggaca ggagaaaact
      601 gttgatgcag ccaaaatttg tggcggcgca gaaaatgttg ttaaaacaga aacccagcaa
      661 acattcgtaa atggattgct cggttttatt actttaggca tttatactcc gctggaagcg
      721 cgtgtgtatt gctcacaata attgcatgag ttgcccatcg atatgggcag ctctatctgc
      781 actgctcatt aatatacttc tgggttcctt ccagttgttt ttgcatcgtg atcagcctct
      841 ctctgagggt gaaataatcc cgttcagcgg tgtctgccag tcggggggag gctgcattat
      901 ccacgccgga ggcggtggtg gcttcacgca ctgactgaca gactgctttg atgtgcaacc
      961 gacgacgacc agcggcaaca tcatcacgca gagcatcatt ttcagctttc gcatcagcta
     1021 actccttcgt gtattttgca tcgagcgcag caacatcacg ctgacgcatc tgtatgtcag
     1081 taattgccgc gttcgccagc ttcagttctc tggcattttt gtcgcgctgg gctttgtagg
     1141 taatggcgtt atcacggtaa tgattaacag cccatgacag gcagacgatg atgcagataa
     1201 ccagagcgga gataatcgcg gttactctgt tcattgctga ccccacaaac agatttcacg
     1261 ctcaatctca cgacgagtca tgagaccttc ccattgctta ccgccagcat atgtccagcg
     1321 acgtagctga tcacatgcgc ctttgatatc gccctggttt attttgcgaa gaagcgtcga
     1381 tgttctgaaa ttgccagcac ccacgttgta aacgaatgag taaagagcgc cgcgcgttgt
     1441 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     1501 attcaagagt gctttgcatt ctgctttggt atacgtttta ccgagcatga tgtcttttcc
     1561 tgtatgcccg tgacatacag tccatacacc aacaatatct ttgtatggta tgtagctgac
     1621 accttccaga ccatcgttac cacttgggcc agtgattaac actgatgcta tagcaattgc
     1681 tccgccacca atagcagcag caacggcttt tcgtaatgat ggaggcatta ttcacctctc
     1741 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     1801 ccaaatacca ggctacccag cacacctatt gctgcccact gtgagggcgt gactttatcg
     1861 agcagctgta aaaaccagta accggcacta cctgctgagg tgccataggc gacacccgtt
     1921 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     1981 gaatacaacg tatcgcaaaa aagcagaaac gtaacagact cggagtcagt gaataactta
     2041 ggtattgagt tatcagctaa tatcgagact caaaaaatgg aaaaaccagc tcgacggcgg
     2101 gtttaagctg tgtgacgaag taaccactct taacagcata accaattttt tacgtacgta
     2161 aaccactgaa tgatatttat gagaatgcta ccgagtgttc aaaacaccac cacaaataca
     2221 taagaaaacc tcaacaaata accaat
//
LOCUS       DAZUCE010000393         2241 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZUCE010000393 DAZUCE010000000
VERSION     DAZUCE010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.395"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(129..954)
                     /locus_tag="ACHY03_005084"
                     /pseudo
     CDS             complement(129..954)
                     /locus_tag="ACHY03_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1105..1878)
                     /locus_tag="ACHY03_005085"
     CDS             complement(1105..1878)
                     /locus_tag="ACHY03_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3908180.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEALKECLRPNRRVEITGQSVR"
     gene            complement(1903..>2241)
                     /locus_tag="ACHY03_005086"
     CDS             complement(1903..>2241)
                     /locus_tag="ACHY03_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3908181.1"
                     /translation="SQLHATNRQVAGNTQAINTLGNKFSQLSYRVEELNRDIRGVGAS
                     AAAMSGIPQAYLPGKSLMGLGVGGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNF
                     SVGAGVGWQW"
BASE COUNT          517 a          647 c          474 g          603 t
ORIGIN      
        1 tattccagag atttcacatt agtctccaat ttttcagaag aatctattta cctggcagga
       61 aaagtcatct ggtgtttcgc cggagtgagg ggtacaggca atagcggaca taaccatttg
      121 ttctttttct aagcagccat ctgataatat tttatcctga aggctgtcgg ggagatattc
      181 cccagacgag agtgacgacg ctgacgattg tagaaaatct caatgtattc ccgtattact
      241 gagatggcct catcccggct tttaaaacga tagtggctca gactttcatt tttcagcgtt
      301 ccccagaagc tttccatcgg agcattgtcg taacagttaa ctttacgcga cattgatatt
      361 ttcagaccaa actgctcctg tatggccctg taatcgtatg cgcagtactg tgaaccacga
      421 tcggagtggt ggattagccc ggtaggtgag cgctggttcc tgagcgccat aaacagggct
      481 ttacccgtca actatttcgt catgcgctct cccatggcgt aaccgacaat tttgctcgta
      541 taaacatctt tgacgccagc gatatacagc catccttccg gggggcaaca tacgttatat
      601 ctgcaaccaa gacctgatta ggtgctgtag gcgtaaagtt ctgattcagg agattcggcg
      661 caaccggcag attgtgacca gagttcgtgt tcgccctgaa cttgcgtttc tgcttacagt
      721 gcagcctcag ctccttacga agacgtgcca gtctgtcacg accgacgata attccgttat
      781 ctgccagctc cgtctggagc agctaggttc catatgtttc gcgtgtgcgg atatgtgcca
      841 ccttaatctc cagttttagc cgctcatcac tttgttttct gtctgagggt catgctgtac
      901 tcagtcgtaa taaccgatcc tgtatacacc aagtacccga cacatcgctt caatacataa
      961 tcgcatctga atcgttcacc gattcgcggc atttttcaag gtagttgtcg ccattgttcg
     1021 actaactgat cgacattaca gcctagagcc tagctggggg ctgtgcacaa aaaagccccg
     1081 gacattccgg ggctacactg catattaacg aacgctctgt ccggttattt caaccctgcg
     1141 gttggggcgc agacattcct tcagtgcttc gccctccaca tccgggcaga acaccactgg
     1201 tgcatcatat ccacggcccg ttgcatgtat cctttcagcc ctgaatcccc gggcaataaa
     1261 ccatcccctg actgattccg cccgtttctc tgacaggagc cggttatacg cctgcccgcc
     1321 aagatggtcc gtaaatcctt ctatcttcac cccgacaaca tgacccgcct gaaacttctc
     1381 tgccacacgg gaaagtgcat cacgccccct ctctgtcaga gtccatgaat caaaatcaaa
     1441 taatatatca ccggggactg actccctgta ttctgctgtc tctgcctccg gcagacagtt
     1501 ttccggcttc cagtagaaag agcgggcatc catgttccgg tcaaacagta tcttgtactg
     1561 gcaggtataa tccccctccg gtccggtaaa gttgaacagg taatcccatt cccttacacc
     1621 agacatccct tcagcaaagt gtggtctccc cgccagctga taaatctggt ccttattcat
     1681 acccgccttt atctgacgta acttttctgg cagtggccac gaacctttct gtgttccgtc
     1741 atgattaaac atcaccttct ccggagaagg ccataccact tcttccgtcg ttccttcatc
     1801 cgtgacatga ctcagatttc cacatcctgc aagcatcagg gacagcacac ccgtcatcat
     1861 cattcgacta aataacatta catttctcct tgcttttccc tgttaccact gccagcccac
     1921 accggcaccg acggagaaat taccccgggt gttctgacct gcattcagtt tcattatcat
     1981 cttaccgtta tcggagattc gggacacacc tattgcaatg gcgctttcac caccatatcc
     2041 accgacaccc agccccatca gacttttacc cggcagatag gcctgaggta tcccgctcat
     2101 ggccgcagcc gatgcgccaa caccacggat atcccggtta agctcttcca cccggtagct
     2161 cagctgactg aatttattcc cgagagtgtt tatcgcctgt gtatttccgg caacctgacg
     2221 gttagttgca tgcaactgac t
//
LOCUS       DAZUCE010000394         2240 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZUCE010000394 DAZUCE010000000
VERSION     DAZUCE010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.396"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            58..390
                     /locus_tag="ACHY03_005087"
                     /pseudo
     CDS             58..390
                     /locus_tag="ACHY03_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332514.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(681..1154)
                     /locus_tag="ACHY03_005088"
     CDS             complement(681..1154)
                     /locus_tag="ACHY03_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908182.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRVILAREFFIPENRKSVPTHD
                     ESWVIICDAMVSRLMRCMFQGRRYLQLDAEEITGRLETDRKIRNGDWEQNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGIHTKTVNTFLYMGLGKNGLRYRSVKHLVGRA"
     gene            complement(1242..>2240)
                     /locus_tag="ACHY03_005089"
     CDS             complement(1242..>2240)
                     /locus_tag="ACHY03_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFS3908183.1"
                     /translation="NKDDSNIKQMVAQAAGNSGRYTVSLPSGLPSLTTENIFNLKVTD
                     IEKIKSNIDGVNSVITSKTAKDYNIAISNGMSSEAALTAAKDGNGALLQEFNRLGTNV
                     NDLKNATTFALDENGEITYEKGVESIPVKDVVAEVKADTTIRKNKDGSYTLDQTAPDT
                     VRVNAAIVDLDKNLKDRTTVGVNSDGSLTTAEGATKTVAVSEGLVSLSGRTGRIDAAV
                     GGIDTRVTKNTQTIQNHSRQLQEHNARLNSQQRQIRENHEEMKRAAAQSAALAGLFQP
                     YSVGKFNATAALGGYSDKQAMAVGVGYRFNEQTAAKAGIAASDGDVSYNVGVNFEF"
BASE COUNT          548 a          603 c          447 g          642 t
ORIGIN      
        1 tctgataaaa tattgtctgt tatccattat cattactaat acttgcgtag tgaatttatg
       61 aacaatccta atccatgcgt gatgtgtggt gcctgttgtg catttttccg cgtctctttt
      121 tactgagctg aagcagatga tgctggcggt accatccccg taacactgac agaacagatt
      181 tctccgtacc atcgatgcat gcagagcact aaccagaaaa atcctcgctg tgtggtcctg
      241 agcggtcaac cgagagtcaa tgcctgctac agcatttatg aaaatcgctc atccacctgc
      301 agggaatttc ctatgtccgg caacaatggt gtggttaatg aagcatgtaa ccgtgcgcga
      361 gcgaaatacg acctaacccc actttactaa acgcgatcgg tatcgctgtc atgacatatt
      421 ttgtatgcca ttattctgac gacttaacaa ctcacttatt gtaatcaagt ttctttcttc
      481 tggtcgggga gctgtgcagc tccccttttt tattgcatca ttaaggcagc cactgcacag
      541 agcgttacca ccgtacccag ccacacaaaa agcatgaaac acatcgctcc tgctgtgtag
      601 cgcttcttta cgtatttcag atttgcctgt ttcatgacaa tttaccagat tattcttcac
      661 ccccggataa caaaacgcgg ctatgcccgc cccacaagat gcttcacact tctgtacctc
      721 aggccgtttt taccaagccc catatacaga aaggtgttca ctgtcttcgt gtgaattccc
      781 atttcacggg caagatgaca cggctttgac tcccggtata cataaccaaa catcaccacc
      841 atctctgaca tcgtcagcgg acgtgcgtat gtgttctgtt cccagtcacc gttacggatt
      901 ttcctgtccg tttccagacg ccctgtaatc tcctccgcat ccagttgaag ataacggcgt
      961 ccctgaaaca tacagcgcat cagacgactc accatagcat cacagataat cacccaggac
     1021 tcatcatggg tcggtacact cttcctgttc tccgggataa aaaattccct cgccagtatc
     1081 accctgtaac cttccagcat ctgttgcaga ccgtactcca gataacggtt acgggtcgcc
     1141 agaatcactt tcatcgcagc cccctgtttc atgctcacat gagaaaaaaa cggcaggcca
     1201 aatcccctgc ctgccgtcat gcttcatgtc actgtccgca attaaaactc gaagttcaca
     1261 ccaacgttgt aggatacatc accgtcgctg gctgcaatac ctgctttggc tgctgtctgc
     1321 tcgttaaagc ggtaacccac accaacggcc atagcctgtt tgtcactgta accacccagt
     1381 gctgccgtgg cgttgaattt acccacactg tacggctgga acaaacctgc cagtgccgca
     1441 ctctgtgcag cagcacgttt catttcttcg tggttttcac gaatctgacg ctgctgactg
     1501 ttcagccgcg cgttatgctc ctgcagctgg cgggagtgat tctggatagt ctgagtgttt
     1561 ttggtgacac gggtgtcaat gccaccaacc gcagcatcaa tacggccagt tcttcccgac
     1621 agggaaacaa ggccttcact taccgcaaca gtttttgtgg ctccttcagc agtggtcagg
     1681 ctgccgtctg aattcacgcc aacagtggtt ctgtctttca gatttttatc cagatcaaca
     1741 attgcagcgt tcacacgaac agtatcaggc gcggtctgat ccagggtata gctgccatct
     1801 ttgttcttac gaattgttgt atcggctttt acctctgcaa ccacatcctt aacaggaatg
     1861 ctttccactc ctttttcata ggtaatttca ccattttcat caagagcaaa tgtagtggca
     1921 tttttcaggt catttacatt tgtgccgaga cggttgaatt cctgtaacag cgctccatta
     1981 ccatctttag ccgctgttag cgcagcttct gaactcatgc cgttactgat agcaatattg
     2041 taatcttttg cagttttact ggtgataact gaattaacac catcaatatt agattttatt
     2101 ttttcaatat cagtcacttt tagattgaaa atattttcag tcgttaacga agggagccca
     2161 ctgggtaaag agactgtata acgccccgaa ttaccagcag cctgagccac catctgttta
     2221 atgtttgaat catctttatt
//
LOCUS       DAZUCE010000395         2198 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZUCE010000395 DAZUCE010000000
VERSION     DAZUCE010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.397"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..928
                     /locus_tag="ACHY03_005090"
     CDS             <1..928
                     /locus_tag="ACHY03_005090"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFS3908184.1"
                     /translation="CGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENT
                     IGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVW
                     DFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFS
                     RPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIP
                     AVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFA
                     ELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1298..2122
                     /gene="gadX"
                     /locus_tag="ACHY03_005091"
     CDS             1298..2122
                     /gene="gadX"
                     /locus_tag="ACHY03_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFS3908185.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMKRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQRLSNRDSAASIVAQGNFYGADCSAEGIRL"
BASE COUNT          572 a          491 c          520 g          615 t
ORIGIN      
        1 gtgcggtccg gtgcaaatct gctggcataa attcgcccgc tactgggatg tggagctgcg
       61 tgagatccct atgcgccccg gtcagttgtt tatggacccg aaacgcatga ttgaagcctg
      121 cgacgaaaac accatcggcg tggtgccgac tttcggcgtg acctacaccg gtaactatga
      181 gttcccgcaa ccgctgcacg atgcgctgga taaattccag gccgacaccg gtatcgacat
      241 cgacatgcac atcgacgccg ccagcggtgg ctttctggca ccgttcgtcg ccccggatat
      301 cgtctgggac ttccgcctgc cgcgtgtgaa atcgatcagt gcttcaggcc ataaattcgg
      361 tctggctccg ctgggctgcg gctgggttat ctggcgtgac gaagaagcgc tgccgcagga
      421 actggtgttc aacgttgact acctgggtgg tcagattggt acttttgcca tcaacttctc
      481 ccgcccggcg ggtcaggtaa ttgcacagta ctatgaattc ctgcgcctcg gtcgtgaagg
      541 ctataccaaa gtacagaacg cctcttacca ggttgccgct tatctggcgg atgaaatcgc
      601 caaactgggg ccgtatgagt tcatctgtac gggtcgcccg gacgaaggca tcccggcggt
      661 ttgcttcaaa ctgaaagatg gtgaagatcc gggatacacc ctgtacgacc tctctgaacg
      721 tctgcgtctg cgcggctggc aggttccggc cttcactctc ggcggtgaag ccaccgacat
      781 cgtggtgatg cgcattatgt gtcgtcgtgg cttcgaaatg gactttgctg aactgttgct
      841 ggaagactac aaagcctccc tgaaatatct cagcgatcac ccgaaactgc agggtattgc
      901 ccagcagaac agctttaaac acacctgata acataacgtt gtaaaaaccg aatgcccagc
      961 ctttaaaaaa acagctgggc attcggttgc ttattaatcg caataatata ttggctgtct
     1021 attcatcgtg ttaataagat atatgctata tgaatgttta ttaaatgtag cgattatatt
     1081 taatgtcacg aaaaggtatt atttgcttac tattaatttc cctgcgttct taaacacgtt
     1141 ttttgtatta taaatccagt catcctgccc gtagaacatc acacattatc atcctgttct
     1201 cccgcttcgt ttaaattttt tatcaatcaa tttgacttaa gagggcggcg tgctacatta
     1261 ataaacagta atatgtttat gtaatattaa gtcaactatg caatcactac atgggaattg
     1321 tttaattgcg tacgcaagac ataaatatat tctcaccatg gttaatggtg aatatcgcta
     1381 ttttaatggc ggtgacctgg tttttgcgga tgcaagccaa attcgagtag ataagtgtgt
     1441 tgaaaatttt gtattagtat caagggatac gctttcatta tttctcccga tgctcaagga
     1501 ggaggcatta aatcttcatg cacataaaaa agtttcttca ttactcgttc atcactgtag
     1561 cagagatatt cctgtttttc aggaagttgc gcaactatcg cagaataaga atcttcgcta
     1621 tgcagaaatg ctacgtaaaa gagcattaat ctttgcgttg ttatctgttt ttcttgagga
     1681 tgagcacttt ataccgctgc ttctgaacgt tttacaaccg aacatgcgaa cacgagtttg
     1741 tacggttatc aataataata tcgcccatga gtggacacta gcccgaatcg ccagcgagct
     1801 gttgatgagt ccaagtctgt taaagaaaaa attgcgcgaa gaagagacat catattcaca
     1861 gttgcttact gagtgtagaa tgaaacgtgc tttgcaactt attgttatac atggtttttc
     1921 aattaagcga gttgcagtat cctgtggata tcacagcgtg tcgtatttca tttacgtctt
     1981 tcgaaattat tatgggatga cgcccacaga gtatcaggag cgatcggcgc agagattgtc
     2041 gaaccgtgac tcggcggcaa gtattgttgc gcaagggaat ttttacggcg ctgactgttc
     2101 tgcggaagga ataagattat agagttttac tcagacataa aaaaaacccg gcatagggga
     2161 ccgggaagag gatagtctgc cgtctccaga ctaataaa
//
LOCUS       DAZUCE010000396         2183 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZUCE010000396 DAZUCE010000000
VERSION     DAZUCE010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.398"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..195
                     /locus_tag="ACHY03_005092"
     CDS             <1..195
                     /locus_tag="ACHY03_005092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908186.1"
                     /translation="KNRFCDKIANTPRISNRETTAEYAHELPELTNHSALAHASLCLE
                     LIFYIRLKKNKNQKMSMIHY"
     gene            1128..2132
                     /locus_tag="ACHY03_005093"
     CDS             1128..2132
                     /locus_tag="ACHY03_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFS3908187.1"
                     /translation="MQNVALIGIDLGKHFFHIHCQDKSGNALLRKKFTRTKLMQFLVT
                     CPSSVVVMEACAGAHFMARRISDIGHDVKLISPQFVRPFVKSNKNDFIDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLMKLHAHYLYLIEQITELESELNQSIKADATAQRIMTIPGV
                     GLITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFMMRLEHQQGRLAEWVRKQLDNKHSNIVACALANKLARIAWAITVLQKEYQA
                     "
BASE COUNT          658 a          462 c          454 g          609 t
ORIGIN      
        1 aagaatcgat tttgtgataa gattgctaac actccacgca tctctaatcg cgagacaact
       61 gctgaatatg cacatgaatt acctgaactt accaatcact ctgcacttgc gcatgcctct
      121 ctctgccttg agttaatttt ttatattaga ttgaaaaaga ataaaaatca aaagatgtcg
      181 atgattcatt attaattctc accactagag gcgaaggtga tttggcaccg ccgcattatc
      241 gtaaagaact tctaggattg ctcaataccc ttttatcgat taaaaagtga tgcccggaat
      301 tccaagcagc tatagagcct gctattcaag caagtccgaa ttaggtaaat aaaccaccta
      361 catagcgggt ggtttagata tagatctcta agtaagccta aaaaaataga tgtacttctg
      421 aatgttgatc gattgtaatc taggtaacct agtcgagctt aaaattttct cagcgtaaac
      481 aagaagtttt tcaaatattg aatcatactg aacctcccac tttttataag gttatggaaa
      541 tgttccgtac cagtaagttc gataattagt gttgttcata ttagtcgtta tgaaaaattt
      601 tgaactttcg ctcttcgctc acagtgaaaa ttcagttcag tcagttcggc tacttagtgc
      661 caagaacgga cgcaaccaat tagggtatta aggtgcggat ttcaattcac tcagcggaat
      721 cagccttgga actttgcctg acgttcaaat atctcaatag ggtatatccg aagtatcaac
      781 gatcagttga tattcatgaa agttcatttc atcctgaacc ttcctagata ttctttcagc
      841 aaacatacag agagaatcac tggctaagtc ctgattgtta agtggtccta ttttaatgga
      901 tttgagcaaa tgaggctcaa atgacacacg gatatatgga attaaaatat cacctcttgc
      961 tctaaatgag atccgctcat catcaggtgg acaactcaaa acaaacctga cttcactttc
     1021 atcatcgaag gcctgatgtt taaatgaaag tgcatagata atgagatggt gacccccacc
     1081 ctgtgagcgg tacgctgaca gggtggatta ttttagaggg caccatcatg caaaacgttg
     1141 cgcttattgg tatcgatctc ggtaaacatt tttttcatat tcattgtcag gataaatctg
     1201 gcaatgcgct tttgcgtaag aagtttacgc gtacgaagct gatgcagttt ctggtaacat
     1261 gtccatcctc agtagttgtt atggaagcct gtgctggtgc gcattttatg gcccggcgta
     1321 tcagcgacat cggtcatgac gtaaaactta tctctccgca attcgtgcgt ccatttgtca
     1381 aaagcaataa gaatgatttt attgatgcag aagccatatg cgaagcggct tcacgaccat
     1441 caatgcgttt tgtccagccc agaacagaaa cccaacaggc aatgagggca ttacaccggg
     1501 tcagagaatc gctcatcagg gataaggtga aaacaaccaa ccagatccac ggtttcctgc
     1561 tggaatttgg aatcagcctg ccgacagggg atgccgtaat aaaacggttg tctctggtgt
     1621 tggctgaaca cgaaatacct gaatatctga gtcgcttgct gatgaaatta catgctcact
     1681 atctttatct tattgagcaa ataacagaac tggaatcaga gttaaaccag tcaatcaaag
     1741 ctgacgctac tgcgcaacgc attatgacaa tacccggtgt tgggctgatt acggccagcc
     1801 tgctctcatc ccagcttggt gatggtaaac aattttcatg cagcagggat tttgcagcat
     1861 ccacaggact tgttccccga caatatagta ccggtggtaa atcaacgctg ctggggatca
     1921 gcaaacgagg cgacaaaaat ttgcgtcggt tacttgttca gtgcgcccgc tcgttcatga
     1981 tgcggctgga gcatcaacag ggcagactcg ctgaatgggt tcgaaaacaa ctcgacaaca
     2041 agcattcgaa tattgtcgcc tgcgctctgg caaataaact ggcccgaata gcctgggcaa
     2101 tcactgttct gcaaaaagaa tatcaggctt gatatatact gacaaaactc aattagctta
     2161 ttttaaacaa taagatacaa gtt
//
LOCUS       DAZUCE010000397         2147 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZUCE010000397 DAZUCE010000000
VERSION     DAZUCE010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.399"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..850
                     /locus_tag="ACHY03_005094"
     CDS             <1..850
                     /locus_tag="ACHY03_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFS3908188.1"
                     /translation="AEFNKVITDVGPGKLNLGGCAIEVMGTYNAAIVRVTIGEYGGDG
                     FLNGTVCTCTVYGDTQRFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSSDTDSNDET
                     KAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDTGPAGPAGPQGPEGDTGA
                     AGPAGAQGPKGDKGDPGEAGPAGPAGPKGERGEPGPMGPPGKDGATSLVGGLRIGGKK
                     EVAHHEVITFPSLDGAQPTIPGMNIVPDGAVILNIRSQAYKITAVTYGFLQVHNGSQW
                     VTIKTE"
     gene            914..1585
                     /locus_tag="ACHY03_005095"
     CDS             914..1585
                     /locus_tag="ACHY03_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFS3908189.1"
                     /translation="MIIKNLKHYTPEKPDVPGAMYLKSEDGQDWYECQSLFSAETLKL
                     VYDSNGIITSISKDVSMLWPVNQSVAEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     GWAQE"
     gene            complement(1749..2078)
                     /locus_tag="ACHY03_005096"
     CDS             complement(1749..2078)
                     /locus_tag="ACHY03_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFS3908190.1"
                     /translation="MNNPNPCMTCGACCAFFRVSFYWAEADDAGGKVPVSLTEQISPF
                     HRCMRGTSQKNPRCVALAGTPGKNAYCSVYKNRPSTCREFAMSGENGIANEACNRARA
                     KYGLPEI"
BASE COUNT          596 a          463 c          652 g          436 t
ORIGIN      
        1 tgcggaattt aataaggtca tcacggatgt cggaccggga aaactcaatc tgggaggctg
       61 cgccattgag gtgatgggga cgtataacgc agccatagtc cgggtcacca tcggcgaata
      121 cggtggcgat ggttttctga acggcacggt ctgtacctgt acagtttacg gagacacaca
      181 acgttttcac tggcgggtgg attacagcac aaaaaacaaa ccggatacgg tcagccagcg
      241 ggatgccagc acgacgcaga aaggtgtggt gcagttaagc agtgatactg acagtaatga
      301 cgaaacaaag gcagccacgc cgaaggccgt gaaggcggca atggatgtgg caaatgaagc
      361 gaaaacaaag gcagaagagg ctgcagcagg aggtggtgtt cccggtccga aaggagagaa
      421 aggggacacg gggccagcag gtcctgcagg accgcaggga cctgaagggg atacgggagc
      481 tgcaggcccg gcaggcgcac aggggccaaa aggtgacaaa ggcgatccgg gagaagcagg
      541 tccagcaggt ccggcaggtc ccaaaggtga gcgcggtgaa ccgggaccga tggggcctcc
      601 ggggaaagat ggcgcaactt cgctggttgg tgggctgcgt atcggaggga agaaagaagt
      661 cgcacaccat gaggtgatta ctttcccgtc actggatgga gcacaaccaa cgattcccgg
      721 catgaatata gtccctgatg gtgctgtaat tctgaatatc cggtcacagg cttacaaaat
      781 tactgctgta acttatggct ttcttcaggt tcacaatgga agccagtggg taacgattaa
      841 aacggaataa cgtgactcac actggttgtt gcgttccatt atgcccaacc gggcattaaa
      901 tacggagaat aatatgatta ttaaaaacct gaagcactat acccctgaaa aacctgatgt
      961 gccgggtgcg atgtacctga aatcagagga tggtcaggac tggtatgagt gtcagtcgtt
     1021 attttcagcg gagacgctga agctggttta tgacagtaac ggtataatca ccagcataag
     1081 taaggatgtc tctatgctgt ggccggtaaa ccagagtgtg gcagaggttg cagatacgga
     1141 agaaaaccgc aaagctgaca tctcggggcg ctggggtttt gacggggaga aaatcacgga
     1201 cctgctgacc gcggagaaag cgcgagggat gaagggcgat gaaattaacg cctggcgtaa
     1261 tgcgatggaa gcggcgaact acacgtttga gcacaatggg cgtaaatggg actacgggaa
     1321 gtcaacgcag acgcgtcttg agccatcggt ggcggcggcg aaagcgggga aacttccgga
     1381 ggcgtttttc tggacggatg cggaaaacaa tgatgtgccg gtgacagcag aagagcttat
     1441 tgcgctgagt gaagcggcag agcaggcgat gttcaccaaa gggatggaaa tccacgttcg
     1501 ccagcgcacc atgaagaaag agctggaaaa gctgaccagt gcggatgaga tactggcata
     1561 cagggttggc tgggcacagg aataacagac aaaaagatgg gggaccatca cctcccccat
     1621 gaactgcgtt gattatgtca ttattgtgaa gatatacact atgccgggta aatagtgcca
     1681 cggaaaagtt aattccggaa gtgacggaac actcattttt cgcgcatcct gttgatatgg
     1741 atgatttttc agatttccgg aagtccgtat tttgcccggg cgcggttgca tgcttcattt
     1801 gcgatgccat tttctccgga cattgcgaat tcccggcacg tggacggacg gtttttgtaa
     1861 acagagcaat atgcgttttt acccggagtt cccgccagag cgacacatcg gggatttttc
     1921 tgactggtgc cgcgcataca gcgatgaaag ggggatattt gctcagtgag actgaccgga
     1981 acctttccgc cagcatcgtc agcttctgcc cagtaaaaag agacgcggaa aaatgcacaa
     2041 caggcaccac acgtcatgca tggattgggg ttattcataa ttcatcggta tagcgataaa
     2101 tcgtaaagag aacaatgtac ctagtgtgta ttgagctaaa tatacca
//
LOCUS       DAZUCE010000398         2136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZUCE010000398 DAZUCE010000000
VERSION     DAZUCE010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.400"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            67..450
                     /locus_tag="ACHY03_005097"
     CDS             67..450
                     /locus_tag="ACHY03_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaA family stress response protein"
                     /protein_id="HFS3908191.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSAVLSDSAVMAMLKQK"
     gene            complement(514..957)
                     /locus_tag="ACHY03_005098"
     CDS             complement(514..957)
                     /locus_tag="ACHY03_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFS3908192.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGA"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="ACHY03_005099"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="ACHY03_005099"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFS3908193.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          539 a          570 c          478 g          549 t
ORIGIN      
        1 aaaaaccccg gtccataagg ccggggtttt ttgcatatca attacttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagacgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattctcc atgaagtggt cgctggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagcggtact cagtgatagt
      421 gcggtcatgg caatgcttaa gcagaaataa tcgtgtcacc attggtgggt acgaaacctg
      481 aagttcagcc caccgggatg agaaaaaaat cacctacgcc cccacatatg ccagattcag
      541 cagcggatac ggtttcccca gatcgtccac ttcagagcgt cccgtaacct taaaacccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acattggttg tcagttccgg
      661 tgccatcgag agcgcatgct ccaccagcat ccgacccacg ccgcagccgc acacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttagcaac ataaacccaa ccggctgatc
      781 ccgctcatta accgcgaccc acaacggtgc ttccggcaga aaggaacgca ccagctcttc
      841 cagttcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcctccc cttcctcatg ccgtgagcgg cgaatactaa taaccatttt
      961 ctctcctttt agtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggcaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtagtg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtaa tgacaacttc tcgtgcgtct
     1201 ggtcaggaaa ttcgtccact taaggttctg atccttaacc tgatgccgaa gaagattgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctg
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa caacttctac
     1381 tgtaactttg aagatattca ggaacagaac tttgacggtc tgattgtaac cggtgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgtt gggcggtcca ggccgcgctc
     1561 aatatcctct acggcattcc taagcaaacc cgcaccgaca aactctctgg cgtttacgag
     1621 catcacattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattacac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgaatt
     1801 gcctttgtga cgggccatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccggactaga cccggaagta ccgtataact atttcccgca caatgatccg
     1921 caaaacaaac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatac gatctacggc acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcacc ttacctcagg caccttcggg
     2101 tgcctttttt atttccgaaa cgtacctcag caggtg
//
LOCUS       DAZUCE010000399         2063 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZUCE010000399 DAZUCE010000000
VERSION     DAZUCE010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.401"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            62..793
                     /gene="artJ"
                     /locus_tag="ACHY03_005100"
     CDS             62..793
                     /gene="artJ"
                     /locus_tag="ACHY03_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFS3908194.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKISDQWFPQ"
     gene            complement(844..>2063)
                     /locus_tag="ACHY03_005101"
     CDS             complement(844..>2063)
                     /locus_tag="ACHY03_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS3908195.1"
                     /translation="THLISDHLHYWEDGGGNYHNRYSSWEIVRGQEGDHWHASVAQPP
                     IPEVLRVPQKQTGGGISGLWRHDWANREYIQQEADFPQTKVFDAGCAFIHKNHAEDNW
                     LLQVETFDPHEPFHTTEEYLSLYEDNWDGPHYDWPRGRVQESDEAVEHIRCRYRSLVS
                     MCDRNLGRILDLMDEHDLWRDTMLIVGTDHGFLLGEHGWWAKNQMPYYNEVANNPLFI
                     WDPRSGVKGERRQALVQMIDWAPTLYDFFQQPVPPDVQGQPLAKTVSHDEPVRSSAMF
                     GVFSGHANVTDGRYVYMRAALPGREDDIANYTLMSCKMNSRYPVDEMRALSLAPPFRF
                     TKGLQVLRIPAQEKYKGLNQFGHLLFDLQNDPQQLHPIHDDVIESRMIALLIQLMKDN
                     DAPPEQFQRLGLA"
BASE COUNT          538 a          566 c          473 g          486 t
ORIGIN      
        1 ttgttatccc atgccgcaga cacggccaat aatcataaga ttgacagacg ggagttccat
       61 catgaaaaag ttagttcttg ccgcattact ggcatcctgt gcgttcggcg cttctgccgc
      121 agagaaaatc aattttggcg tatcagcgac ctatccgccg ttcgagtcga tggatgccaa
      181 taaccagatc gtcggctttg atatcgatct ggcaaaagcc ttgtgcaaac aaatgcaggc
      241 agaatgtact tttactaatc acgcgttcga cagcctgatc ccgtcactga aattcagaaa
      301 atatgacgcc gtaatctccg gtatggatat caccccggag cgtagcaaac aggtatcgtt
      361 taccacgccc tactatgaaa actcagccgt cgtgattgcc aaaaaagata cctacaaaac
      421 gtttgccgat ctgaaaggca aacgtattgg gatggaaaac ggcaccacgc accagaaata
      481 tattcaggat cagcacccgg aagtgaaaac ggtctcctat gacagttatc aaaatgcctt
      541 tatcgatctg aaaaatggcc gtattgatgg ggtatttggt gacactgcgg tggttaatga
      601 atggctgaaa accaatccgc aactgggcgt tgctactgaa aaagtgaccg atccgcaata
      661 cttcggcacc ggcctgggta tcgctgtacg tccggataac aaagctctgc tggaaaaact
      721 gaataacgcg ctggcagcaa ttaaagctga cggtacatat cagaaaatca gcgatcagtg
      781 gttcccgcaa taattattca tttgccggag tgggcgaaca cccactccgg caaaatcact
      841 tctctacgct aatcccaggc gctgaaactg ctctggcggc gcatcattat ctttcatcaa
      901 ctgaatcagc aacgcgatca tccgggactc gatcacatca tcatgaatcg gatgtagctg
      961 ctgcggatcg ttttgcagat caaacagcaa atgaccaaac tgattcaacc ccttatattt
     1021 ttcctgtgcc gggatgcgta atacctgtaa ccctttggta aaacgaaacg gtggggccag
     1081 cgataaagcc cgcatctcat ccaccggata gcggctgttc attttgcagg acatcaacgt
     1141 gtagttggca atatcatcct cacgccccgg cagcgctgca cgcatataca cataacgccc
     1201 atcagttacg ttagcatgac cactgaaaac accaaacatc gccgagctgc gtactggttc
     1261 atcgtgactg accgttttcg ccagcggttg cccctgcaca tcgggcggca ctggctgttg
     1321 aaaaaagtca tacaacgttg gcgcccagtc gatcatttgt accagtgcct gtcgccgctc
     1381 tccttttaca ccactgcgcg ggtcccagat aaacaacgga ttattggcaa cttcgttgta
     1441 ataaggcatc tggtttttcg cccaccagcc atgctcaccg agtaaaaagc cgtggtcagt
     1501 gccgacaatc agcatggtat ctcgccacag atcgtgctca tccatcaggt cgagaatgcg
     1561 ccccaggttg cgatcgcaca tcgacaccag cgaacgatac cggcaacgga tgtgctccac
     1621 ggcctcgtcg ctttcctgca cccggccacg cggccagtca taatgcggtc catcccagtt
     1681 atcttcatac aaggaaagat actcttccgt tgtgtgaaac ggctcgtgcg gatcaaacgt
     1741 ttcgacctgc aataaccagt tatcttccgc gtgatttttg tggataaagg cacagcctgc
     1801 atcaaacact ttggtttgtg gaaaatccgc ttcctgctga atatattccc ggtttgccca
     1861 gtcatggcgc cacagaccag aaataccgcc accggtctgc ttttgcggca cccgtagcac
     1921 ctcgggaata ggcggttgcg caacgcttgc atgccagtga tcgccttcct gtccgcgcac
     1981 aatctcccac gaactgtagc gattatgata attcccgccg ccatcttccc agtaatgcaa
     2041 atgatcgctt atcaggtggg tat
//
LOCUS       DAZUCE010000400         2058 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZUCE010000400 DAZUCE010000000
VERSION     DAZUCE010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.402"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..634
                     /locus_tag="ACHY03_005102"
     CDS             <1..634
                     /locus_tag="ACHY03_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFS3908196.1"
                     /translation="EQMPTLERFHETFVKKAEAMQKIALIQLDPSGKGGKVIVKDRSK
                     RTNISGYFKGFLNVKRVNSEIELCNKLIDALKITFKRHKELLPESIQKSGVNKIYEVL
                     SRADFEYNPEDASPLLTSIFGPLDEKSPILNTFKTQTKNAGIEGESFTIKADGIHKPP
                     RRKIETAESVVISYNEDDRPEIRNLDDGRKQIIVTTARIITDDIDTSKNR"
     gene            606..2039
                     /locus_tag="ACHY03_005103"
     CDS             606..2039
                     /locus_tag="ACHY03_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908197.1"
                     /translation="MTSILQKIDESIQASRKDPEFLASESIKNDSARVLISAKMDKDA
                     LILWQYVYENVPNWIEITVKDDCNDEVKPSSCSEGDKLNITLKCIAFENNRYIFTLEG
                     WNVFLHNDDLVSITNKVNILGLKHGFETLGYSVEPWNHAPEKIEPNETVRTSINLQSY
                     VKFYSSEFMPASDIAPWVLYKMPEEEDNFFKKWLQVSCNMLCRTLVNELLADEKKSIC
                     LTGKPPKKLIYGDPDILLSDYSVLQTVINWIFIEGNEIELKHTFFTSELAREWPEYVS
                     FCEGLPKKLPMAFESAKLLYKAHIRASSRETIKSLSDLRKTLAEDTQKIISQSKDITS
                     ALWKDLALVISLFAIKYALDASKINITNNIFPYMFFALSIYIFISQVTTLFINKNYFK
                     ILDNTRLVWRDKLYGFLDDTDYENLAVTPLREAYKAYQIIATFVVILTLLISFIMIIL
                     GFSMLDEHATNAITSWLSSHLINSYCS"
BASE COUNT          739 a          349 c          364 g          606 t
ORIGIN      
        1 agaacaaatg cctactttag aaagatttca tgaaactttc gtaaaaaaag ctgaggcaat
       61 gcaaaaaata gcgttaattc aacttgatcc aagtgggaaa ggcgggaaag ttattgtaaa
      121 agacaggagt aaaagaacca atatttcagg atattttaaa ggcttcttaa atgttaaaag
      181 agtcaactct gaaattgaac tttgcaataa gcttattgat gcgctgaaaa taaccttcaa
      241 aaggcataaa gaattattgc cagagtcaat tcaaaaaagc ggcgtaaata aaatatatga
      301 agttttaagc agagctgatt tcgaatataa tcctgaagat gcttctccgt tattgaccag
      361 catttttggg ccgttagatg aaaaatcacc aattctcaac acattcaaaa ctcaaacaaa
      421 aaatgctggt attgaaggcg aatcctttac tataaaagca gatggcattc ataaaccacc
      481 aagacgaaaa attgaaactg cagaaagtgt agtaattagt tataatgaag atgaccgtcc
      541 agaaataaga aacttagatg atgggcgaaa acaaattatt gttactactg cgaggattat
      601 tactgatgac atcgatactt caaaaaatcg atgagtctat acaggcgtca aggaaagacc
      661 ctgaattttt ggcatcagaa tcaataaaaa atgattctgc gcgcgttcta atttctgcca
      721 aaatggacaa agatgcactc attctttggc agtacgttta cgaaaacgta ccaaattgga
      781 ttgaaattac tgttaaagat gactgtaatg atgaagtaaa accatcgtca tgcagtgaag
      841 gtgataaact taacattacg ctaaaatgca ttgcattcga aaataacagg tatattttta
      901 cgctcgaagg gtggaatgtt tttctacaca atgatgattt agtatcaata accaataaag
      961 tgaatattct cggtttgaaa catggatttg aaactttagg atattctgtt gagccatgga
     1021 accatgctcc agaaaaaatt gaacctaatg aaaccgtacg aacttcaatc aatttacaga
     1081 gctacgttaa attctattct agtgagttca tgcctgcatc tgatatagct ccttgggtac
     1141 tttataaaat gcctgaggaa gaggacaact ttttcaaaaa atggttacaa gtttcctgca
     1201 atatgctttg tcgcactctc gttaatgaat tattagcaga tgaaaagaaa tcaatttgtt
     1261 taactggaaa gccaccaaaa aaattaattt atggagatcc agacattctg ctttccgact
     1321 attcagtttt acaaactgta ataaactgga tctttattga gggcaatgag attgaactca
     1381 aacatacttt cttcacaagt gagttagcac gagaatggcc agagtatgta tccttctgtg
     1441 agggtttacc aaaaaaactc cctatggcct ttgagtcagc aaaattacta tataaggccc
     1501 acattcgcgc tagcagcaga gagactatta aatctctgtc cgatctaagg aaaaccttag
     1561 ctgaagacac gcaaaaaata ataagtcagt ctaaagacat cacatccgct ctttggaaag
     1621 atctggctct ggttataagt ctatttgcaa taaaatatgc gctagacgca tcaaagatta
     1681 atataaccaa taacatattc ccatatatgt tcttcgcgtt atctatatat atttttatat
     1741 ctcaggttac aactctcttc ataaataaaa attactttaa gatacttgat aatactaggc
     1801 tagtctggag agataaacta tatggttttc ttgatgatac tgactatgag aatcttgcag
     1861 tcacaccatt aagagaggca tacaaagctt atcagattat agccacattc gttgtgattc
     1921 tgactttgct gatttcattt ataatgatca tattaggatt ctcaatgctt gatgaacatg
     1981 ctacgaatgc aattacctct tggctcagtt ctcacttaat caatagttac tgtagttaat
     2041 ttaaggcgag agaaactc
//
LOCUS       DAZUCE010000401         2036 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZUCE010000401 DAZUCE010000000
VERSION     DAZUCE010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.403"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            97..1329
                     /gene="dgcM"
                     /locus_tag="ACHY03_005104"
     CDS             97..1329
                     /gene="dgcM"
                     /locus_tag="ACHY03_005104"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFS3908198.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(1350..1913)
                     /gene="smrA"
                     /locus_tag="ACHY03_005105"
     CDS             complement(1350..1913)
                     /gene="smrA"
                     /locus_tag="ACHY03_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFS3908199.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT          534 a          508 c          481 g          513 t
ORIGIN      
        1 aaatagcgtc tatcattatc agaattatct gatcatatga cgtggctttt ttgcgatcgg
       61 atagcaacaa aaattgataa aaataatggg atctcaatga ttacgcacaa cttcaatacc
      121 ctggacttac tcaccagtcc tgtctggatc gtttcgccct ttgaggaaca gttaatttat
      181 gccaatagcg cggcgcgact gttgatgcaa gacctcacgt ttagtcagct acgaaccggt
      241 ccctattccg tctcctcaca aaaagaactg ccgaaatacc tctccgatct gcaaaaccaa
      301 cacgatatta tcgaaatcct cactgttcag cgtaaagaag aggaaacagc attaagctgt
      361 cggcttgttt tgcgagagct gacagaaaca gaaccggtga ttattttcga aggtatcgaa
      421 gcgccggcaa cgctggggtt aaaagccagt cgctcggcaa attatcagcg caaaaaacaa
      481 ggtttttatg cgcgcttttt tctgactaac tctgcaccaa tgttgttgat tgacccgtca
      541 cgagatggac aaatcgtcga tgctaacctc gccgcgctca atttctatgg ttataaccat
      601 gaaacgatgt gccagaaaca tacctgggaa ataaatatgc tcgggcgtcg cgtcatgcct
      661 atcatgcatg aaatttcgca tttacccggc ggtcataaac ctttgaattt tgttcataaa
      721 ctggcggatg gatctactcg tcatgtgcag acctatgccg gaccgattga aatttatggc
      781 gacaagctca tgttatgtat tgtgcatgat attactgagc aaaaacggct ggaggagcag
      841 ctggaacatg ctgctcacca tgacgcgatg accggattac tgaatcggcg acagttttat
      901 cacattacgg aaccaggcca aatgcagcat ctcgccatcg ctcaggatta cagcttgttg
      961 ctcatcgaca ccgatcgttt taaacacatt aacgatctct atgggcattc taaaggtgat
     1021 gaggtgttat gcgccctcgc ccgcaccctc gaaagttgcg ctcgcaaagg cgatttggtg
     1081 tttcgttggg gaggcgaaga gtttgtctta ttgctaccaa gaaccccact ggataccgcg
     1141 ctttcgctgg ctgaaactat ccgcgtaagc gtggcaaaag tgagtatttc gggcttacca
     1201 cgctttaccg tcagcattgg tgtggcgcat cacgaaggaa atgaaagcat cgatgaactg
     1261 tttaaacgcg ttgatgatgc tttgtatcgg gcgaaaaatg atggacgcaa ccgcgtgctg
     1321 gcggcataag ccgcggatgc gtctcgagat caacgactgc gcttagcgtg gcgctcccag
     1381 ttttcttgct tcgcctgcgc cgttttacgt agtgcgacgt aacacgcccc gctgccgcca
     1441 tgatgcggta gcgcggtgca atatgcctga acatcatcaa attcggtcag ccagcgcgcc
     1501 acatagctgc ggacaatatt ggcatgcgat ttatcatccc gccctttacc atgaataatc
     1561 agcacgttac gcaaaccatc cgccagggct tgttgaataa aactgaacac cattttgcgg
     1621 cactcttcca ccggctggcg caaaagattc aggctcgcct gttgcggata tttaccactg
     1681 cgcagcttat ccagcacccc atgttgcaac ccttcccgcc gaaactccag cggctgactt
     1741 agtgggataa tgtcgagaaa tccggtggtg aggaaattat caagctgcag cgtgtcgata
     1801 cgctgcggcg cacgttggtt acgcgttgga tgccagtgga catcggtagc acgtttcagc
     1861 ggctggacat cttccatggc gtcaagaaac agcgatttgt cgtcaaggtt catgttacat
     1921 cctcccgcaa ttaagagcgc gatatgataa ccagaccggg tcggtccaac aacgtattac
     1981 ccaaaattcc agtaataagt tccaaatatt gccgatattt taagcaaaat gcttat
//
LOCUS       DAZUCE010000402         2013 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZUCE010000402 DAZUCE010000000
VERSION     DAZUCE010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.405"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            730..1506
                     /locus_tag="ACHY03_005106"
     CDS             730..1506
                     /locus_tag="ACHY03_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFS3908200.1"
                     /translation="MKRSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPAAHLL
                     VLLGIFGYIMHRTMPGISFPVFLLNGLIPFFIFNSISNRSVEAIDANQGLFNYRPVKP
                     MDTIIARTLLETLIYIIVYVLLMLIVWMSGEYFEIISLLQLVLVWTLLIIISCSVGLI
                     FMVVGKTFPEMQKILPIFLKPLYFISCIMFPLHSIPQQYWTYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            1503..>2013
                     /locus_tag="ACHY03_005107"
     CDS             1503..>2013
                     /locus_tag="ACHY03_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3908201.1"
                     /translation="MIKIENLTKSYRTSTGRHYVFKDLNIELPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDRGKIITNKTISWPIGLAGGFQGSLTGRENVKFVARLYAKPEELK
                     DKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAHYFKER"
BASE COUNT          586 a          356 c          444 g          627 t
ORIGIN      
        1 gttgactgtg ttgtactata ttaatatagg cgtttatatt catatcttta agagtaagct
       61 attagatgta aattactttg gtcaggtcct tcacagcaga aacagaatgt catgaaagat
      121 aaatttacgc agtcacttga tcaactgctt agttttgcgt cttaaaggat tgtaactatg
      181 gcaactgtgg agcgaaaaat cattgggcac gggaaaagtg cggtcatata gaaaagtgct
      241 ctctattcag catctcatgg tttatcagcg aagacagaaa aaaattataa cgatgcgcag
      301 gaatttgtgg aggtttgcta atatagagcg attcgagcat ccctcgttaa atctctcgaa
      361 ccgcagtata ttcagacatt tttaaaatac gccagatgat tacgacagaa agaacacaac
      421 atataattca tgttcgctgt cctcttgctg aatacggaat tgtcttcaat aaaggtgcta
      481 cacattatgg tgagagctac cacggtttct tgaggatgca gaaaatggat ttaccgatat
      541 cgtgcaattt tgtatcggta gtatgtccga ctcatgtctc atggataatg aaccatatat
      601 tataactgcg ggtagaaaaa tgcgcatcta acgactttgc ctggacgtgt cgattaactg
      661 ttcctgggtt tgctactctt gtcagtcaga cgataaaagt gagcataaat atataggatt
      721 acatatcaag tgaaaagaag tggatttgaa gttcagaagg tgacagtaga ggcattattt
      781 ctacgagaaa tacgaacacg ttttggtaag tttcgtctgg gatatctgtg ggcaatcctg
      841 gaacccgctg ctcatttgct ggtgctgtta ggtatttttg gctatattat gcatcgtacg
      901 atgccaggta tctcgttccc ggtattttta cttaatggtt tgattccctt ttttatcttt
      961 aatagtatca gtaatcggtc tgtggaagct attgatgcaa accaggggct ttttaattat
     1021 cggccagtaa agcccatgga tacaataata gccagaaccc tgcttgagac actaatttac
     1081 attatagttt atgtgttgct tatgcttatt gtctggatgt caggagaata ttttgaaata
     1141 ataagtttat tacaactggt acttgtatgg actcttctga ttattatttc atgtagtgtc
     1201 ggattaatat ttatggtcgt tggtaaaact ttccctgaaa tgcaaaagat tctaccgata
     1261 tttcttaaac cgctgtattt catttcctgt atcatgttcc ctctacattc gatcccacag
     1321 caatactgga catatctact ctggaaccca ttagtccatg tcgtagagtt aagtcgtgag
     1381 gcagttatgc cgggctatat cagcgaaggc gtgagtttga actatctggc aatgttcact
     1441 ctggtcaccc tgttcatcgg tctggcgcta taccgaacgc gtgaagaggc aatgctgaca
     1501 tcatgattaa gattgagaat ctgacgaagt catatcgcac gtcaacaggt cgacactatg
     1561 tgtttaagga tttaaatatc gaactccctt caggaaaaag tgttgccttt attggtcgta
     1621 atggtgcagg taaatcaact ttactgagaa tgattggcgg tattgaccgc cccgatagag
     1681 gaaagatcat caccaataaa acgatatcat ggcccattgg gctcgccggt ggattccagg
     1741 gcagtttaac cgggcgagaa aacgtaaaat ttgttgcacg gttatatgcg aagccagaag
     1801 aactgaaaga taaaattgag tttgtcgaag aatttgccga actcggtaag tactttgata
     1861 tgccgatcaa aacttactca tctggtatgc gatctcgcct gggttttggt ttaagcatgg
     1921 catttaagtt tgattattat attgtcgatg aagtaacggc agtcggtgat gccaggttta
     1981 aagaaaaatg tgcacattac tttaaagaaa ggc
//
LOCUS       DAZUCE010000403         2011 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZUCE010000403 DAZUCE010000000
VERSION     DAZUCE010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.406"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            245..>2011
                     /locus_tag="ACHY03_005108"
     CDS             245..>2011
                     /locus_tag="ACHY03_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HFS3908202.1"
                     /translation="MTYQAIFTGWDDLTIEDLLVAYRKAKADSFFENTFPVAIKFAEY
                     EQELLENLQKLLDLLQSEDGFSSNKKLIGKFRLLPKKLTTKKKHESQNGHVHFSNPKR
                     AADHLFNNFDLIPEFRIIGDFPVDSHIISALWINMVGHKFDASLDNCCYGARLKRIRN
                     DELFSNEQDNPFHISAVGSFSPYFQPYQKWRGDGLKAIRDELEKDRDIIAASLDLKSY
                     YHFIDPLAITSDDLYNTLNIKLTEDEKAFTAQLAVFLKHWSDGAAAFGKKIAYKTPVI
                     NGGLVIGLTASRIISNILLHHWDKLVIEKLSPIHYGRYVDDMFLVIRDTGKITNNHEF
                     MLLLQDRLGNDCVYLKNEQKQIWQIQQGEHFQGKTTIQLQSDKQKLFVLQGRAGIDLL
                     DSIEKEIYELSSEHRLMPSPDQLEHSTAAKVLTAAGSVGENADTLRRADGLTIRRLGW
                     SLQLRYVETLARDLPPSEWKEQREEFYQFAYNHILRADNLFAHFSYLPRLLGFAISMN
                     EWQHAEKIVLKAYESINLLASVITSGKEVNINGCKTRAVNDLWRCIKGTLSWLFVDAA
                     TRYYSPDRLFLDKRSKKEECLAD"
BASE COUNT          624 a          398 c          427 g          562 t
ORIGIN      
        1 aatagtttac ttattcatca ttttgtctac ttaaactcga catttaacaa aaaattcaaa
       61 cctacccatt ttcgggaaaa atgtgacaaa tatatgctcg gactggttgc ggggagcgtg
      121 taacatggat acaaatcaag attattgcca gcctcactga tggattactt gtgtcaagag
      181 caccccttca ggcatgaaac ggctggctaa ttctgtacag actgtaatct aaggacgata
      241 acgcatgaca tatcaggcaa ttttcactgg ctgggatgat ctgacgattg aagaccttct
      301 ggtcgcttac cggaaagcaa aagccgatag cttctttgag aatacatttc ctgttgctat
      361 caaatttgcc gagtatgagc aggaattact tgaaaacctg caaaaactct tagatctttt
      421 gcagagcgaa gatggattca gtagcaataa gaagttgatt ggcaaatttc gtttgttacc
      481 gaaaaaatta accacaaaga aaaaacatga atcccaaaat ggacacgtcc acttttctaa
      541 tcctaaacga gcagccgacc atttatttaa taattttgat ctgataccag agtttcgtat
      601 tattggtgac ttcccggttg atagtcacat tatctctgca ctatggatta atatggtcgg
      661 gcataaattt gatgccagct tagataactg ttgctatggc gcgcggctaa agcgcattcg
      721 taatgatgaa ttatttagca atgagcagga taatccattc catatcagtg ccgtgggttc
      781 ttttagcccc tacttccagc cctaccaaaa atggcgtggt gatggcttaa aagctatacg
      841 tgacgagttg gaaaaagatc gtgacattat cgccgcctca ctggatttaa aaagttacta
      901 tcattttatt gatccactgg ctataacctc tgatgatctc tataacacac taaacataaa
      961 actgactgag gatgaaaaag cgtttactgc acagttagca gtattcttaa agcactggtc
     1021 tgacggcgca gcggcatttg gaaagaaaat agcgtacaaa acacctgtta ttaatggtgg
     1081 tctggtcatt ggattaacag ccagtcggat catttcaaat atattgctac accattggga
     1141 taaattagtc attgaaaaac tatcaccaat tcactacggt cgttatgtcg atgatatgtt
     1201 tcttgtaata cgcgatacag ggaaaattac taataatcac gaatttatgt tattgctgca
     1261 agataggctt ggcaatgatt gcgtatattt gaaaaacgag caaaaacaaa tatggcaaat
     1321 acagcagggc gagcatttcc agggtaagac caccatccag ttacaatccg ataagcaaaa
     1381 acttttcgtg cttcaaggga gggctggaat agacctgctc gacagtatcg aaaaggagat
     1441 ctacgagctt tctagtgaac accgcttgat gccttcaccg gatcaactgg aacactcaac
     1501 cgcagctaaa gtccttaccg ctgccggtag tgtaggtgaa aatgccgata ctctgcgccg
     1561 tgcggatgga ttaaccattc gtcgtttggg ctggtcactg caattacgct acgttgaaac
     1621 actggcacga gatctgcctc caagtgaatg gaaagaacag cgggaagagt tttatcagtt
     1681 tgcctacaac catattctta gggctgataa tctatttgca cattttagtt atctgccaag
     1741 gctgcttggc tttgctatca gtatgaatga atggcagcac gcggaaaaaa ttgtacttaa
     1801 agcttacgaa tccatcaacc tgttggcatc ggtgattact tcaggtaagg aagtgaatat
     1861 aaatggttgc aaaactcgag cagtaaatga tctttggcgc tgtataaaag gcacattaag
     1921 ctggctattt gttgatgcag cgacacgata ttacagtcct gacagattat ttcttgataa
     1981 acgttcaaag aaagaagagt gccttgcgga t
//
LOCUS       DAZUCE010000404         2001 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZUCE010000404 DAZUCE010000000
VERSION     DAZUCE010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.407"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(304..1986)
                     /gene="glcA"
                     /locus_tag="ACHY03_005109"
     CDS             complement(304..1986)
                     /gene="glcA"
                     /locus_tag="ACHY03_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFS3908203.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT          589 a          518 c          475 g          419 t
ORIGIN      
        1 ataaacaaaa aattaacaac atttcacaac gtaactatat aaattcgttg gtagttttaa
       61 cgattaagat tgcaaaatca acagatagaa cggtgtttgc tcatcaagcg aacataatta
      121 atacagactt gttttgcgtc ataaaaaaat agatattctc acgctcttaa ttatttagca
      181 ggttatctga acgcaaaaca ttattgctgc atggataata aagcgagaaa taattttcaa
      241 ttaataagac agaatgatgt aaatgaagcc ggatgatatt aacgatcatc cggctttatt
      301 gatttacgag actaacatcc cggtaaacac atacgcctgc aacagcgtga taatgccgat
      361 aacgctggca aaaatcagac tgtgcttcac ggtgtagcgg aacagttcag attctcggcc
      421 caccatgccc gtcgcggcgc aggccacggc gatagattgc ggggagatca tcttgccagt
      481 tacgccgccg ctggtatttg ctgccaccag cagtgtgtca gagacgttga tttgctgcgc
      541 cgtggtcgat tgcagtgaac caaacagggc gtttgaagaa gtgtccgagc cggtaaggaa
      601 tacccccagc cagccgagga acggtgagaa gaacgggaac atcacgcctg tacctgccag
      661 taccagcgcc agcgtggtgg acatgccgga atagttggtg acgaaggcga atgccagcac
      721 catgccaatc gacaatatcg gccacttcag actaattagc gtttcggcaa agacgccaat
      781 ccctttcttg atgcccacgc cgaggatgaa gatagagata atcgccgcaa taaaaatagc
      841 ggtgccgcca gccgagaggg ggtcgaattt aaacaccgca tccattggcg ttggttgggc
      901 gacaatgggt gccgctttca acacttgttg atgcaaatga gggatctgga aattaatcac
      961 cagtgaataa aacgcgccgc ccggagcaaa taacgcttta aacggcttca tggtccagat
     1021 ggtgaccagc accgttaaga ttaaaaacgg tgaccacgct cgaatgattt gccccagact
     1081 atattctgaa ggcacgggac cgccagaaga tggcttattt accaccatcg cacctgcgga
     1141 ttgtcccatg ctgattgccg tttcggtatt tttcggccgc cagactttaa ggaataaagc
     1201 gagtgagacg atactcacca gcgccgaagt aatatccggc agttccggac caatatagtt
     1261 agaggtaaag aactgagtga cagcgaagct tcccccagca accagcgccg ctggccacgt
     1321 ctctttcacc cctttccagc cgtccatcat tgctaccagc cagaacggca caagaaccga
     1381 caggaacggt aattgacgcc ccgccattgc gccaatgtgg aacggatcga ttcccgttac
     1441 ctgaccggcg accagaatcg gcacgcccaa cgcaccaaac gccaccggcg cagtattggc
     1501 aatcagacac agccccgccg cgtataacgg tttgaagccc aggcccacca gcagcgcacc
     1561 ggtaatcgcc accggggcac caaagccagc cgctccttcc agcaacgcac caaaggagaa
     1621 accaatcagt aacacctgca aacgctgatc gtcggtgatg gagataaccg agctgcggat
     1681 aatatcgaac tgcccgctgg caacggttaa tttatacagg aacaccgccg cgacaataat
     1741 ccacgcaatt ggccataatc cataaataaa gccatagccc gcagcagcaa atgccatatc
     1801 aatcggcatt ttaaaggcga atattgcaat caggatagat aatataaggg ttattgctcc
     1861 agcgacatgt cctttcagac gtaataccgc gagtgcaacg aagaagaata ttatcgggat
     1921 cagggcgacc agagcggata gccccagtcc tcccatcggc atatacattt gggtccaggt
     1981 aaccatattg ttcagtctct t
//
LOCUS       DAZUCE010000405         1995 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZUCE010000405 DAZUCE010000000
VERSION     DAZUCE010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.408"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..609
                     /locus_tag="ACHY03_005110"
     CDS             <1..609
                     /locus_tag="ACHY03_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908204.1"
                     /translation="DVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGS
                     NQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGY
                     SVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHL
                     EGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            685..>1995
                     /locus_tag="ACHY03_005111"
     CDS             685..>1995
                     /locus_tag="ACHY03_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFS3908205.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQVHERELILNDNGGRSIHFEPLFPGEISYSRSESFWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHMYLATNSLQGPWWILNWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRATSLSSPAGPRSV
                     SSSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLACYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKGHADGSITRSEYDEA"
BASE COUNT          423 a          518 c          693 g          361 t
ORIGIN      
        1 gatgtgataa acaaccatgc cgaaaaaata ggtaacaacc aggcgatcac cgttaccaat
       61 aaccagatcc agaacattgg cgttaatcag atacagacgg ttggtgtcaa ccaggtggaa
      121 acggtaggca gtaaccagat tatcaaagtg ggatcaaacc aggttgaaaa ggtggggatc
      181 attcgtgcgc tgacggtggg tgtggcttac cagacgacgg taggcggcat tatgaatacc
      241 tcggtggcgt tgttgcagtc ctcacaggta gggctgcata aatcactgat ggtgggaatg
      301 ggctacagcg tcaatgtggg gaataacgtc accttctcgg tgggcaagac gatgaaggaa
      361 aacaccggac aaacagcagt ttattctgcc ggtgagcatc ttgaactctg ctgtggtaag
      421 gcaaggctgg tgctgacgaa ggacggaagc atatttctca atggtacgca cattcatctg
      481 gaaggggagt cggatgtgaa cggtgatgcg ccagtgatta actggaactg tggtgccaca
      541 caacctgtac cggatgcgcc tgtgccgaaa gatttacccc ccggaatgcc ggatatgcgg
      601 caattttgag tcaggtgcaa aaaattaaaa cggaactggt gcagtgacca gatgaaccat
      661 attaaaagca gaagggattg attcatgggc ggaaaaccgg cggcgcggca gggtgacatg
      721 acccgcaagg gactggatat tgtgcagggt tcagcggggg tgctgatagg tgcgccgacg
      781 ggcgtggcct gctcggtgtg tccgggaggg attacctatg ctaacccggt gaacccggtg
      841 ctgggtgcga aggtgctgcc gggcgagacg gaccttgcgc tgcccggccc gctgccgttc
      901 atcctttccc gcgcctacag cagttaccgg accaggacgc ccgcgccggt gggggtgttc
      961 ggtcccggct ggaaagcacc cttcgatatc cgcttacagg ttcatgagcg ggaactgata
     1021 ctcaacgaca acggcggcag gagcattcac tttgagccgc tgtttcccgg cgagataagc
     1081 tacagccgca gcgagtcgtt ctggctggcg cggggcgggg tggcggagca gcacagttcg
     1141 cagccgctaa gcgcgctctg gcaggtgctg ccggaagatg ttcgcctgag tccgcatatg
     1201 tacctggcga caaacagcct tcaggggccg tggtggatac tcaactggcc ggagcgggtg
     1261 ccgggggcgg acgaggtgct gccgccggag ccgcccgcat accgggtgct gacgggcgtg
     1321 gtggacggct ttggtcggac gctggccttc caccgggcgg cggagggtga cgtggcgggc
     1381 gcggtgacgg gggtgacgga cggcgcgggc cgccgttttc acctggcgct gaccacacag
     1441 gcgcagcggg cggaagcctt ccgtaaacag cgcgccacgt ctttatcttc ccctgccggt
     1501 ccccgttctg tttcctcttc ttcagctttc cccgacacgc tgcccgccgg tacagaatac
     1561 ggtgccgata acggtatccg gctggaggcg gtgtggctga cacacgaccc ggcatacccg
     1621 gatgaacagc cgactgcgcc gctggcgtgc tacacgtaca cggccagcgg agaactgcgg
     1681 gcggtgtatg accgcagcgg gacgcaggtg cgcgggttta cttatgatgc ggagcacgcc
     1741 gggcggatgg tggcgcacca ttatgcgggg aggccggaga gccgctaccg gtatgatgac
     1801 accggtcggg tgacagaaca ggtcaacccg gaggggctgg actaccgctt tgagtacggg
     1861 caggaccgtg tgaccatcac ggacagcctg aaccggcggg aggtgctgta cacggagggc
     1921 gaaggcggcc tgaagcgggt ggtgaagaag gggcatgcgg acgggagcat cacccgcagc
     1981 gagtatgatg aggcg
//
LOCUS       DAZUCE010000406         1948 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZUCE010000406 DAZUCE010000000
VERSION     DAZUCE010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.409"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..700
                     /gene="bcsQ"
                     /locus_tag="ACHY03_005112"
     CDS             <1..700
                     /gene="bcsQ"
                     /locus_tag="ACHY03_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFS3908206.1"
                     /translation="TAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARAM
                     LDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRYQW
                     ILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIGSQ
                     VQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANWCL
                     LNYSGLKTPVGSAS"
     gene            697..>1948
                     /locus_tag="ACHY03_005113"
     CDS             697..>1948
                     /locus_tag="ACHY03_005113"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS3908207.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYG"
BASE COUNT          416 a          515 c          542 g          475 t
ORIGIN      
        1 caccgccgca ttagcctggt cattacaaat gttgggagaa aatgtcctgg tggtcgatgc
       61 ctgcccggac aacttgttgc gcctgtcatt taacgttgat tttacccacc gtcagggctg
      121 ggccagagct atgctggatg gccaggactg gcgtgacgct gggttgcgct acacctcgca
      181 gctcgatttg ctaccttttg gtcagttatc cattgaagaa caagaaaatc cacagcactg
      241 gcaaacccgg ctgagcgata tttgctccgg cttacagcaa ctaaaagcca gcgggcgtta
      301 ccagtggatt ttaatcgact taccgcgtga tgcctcgcag ataacccacc agctgctgag
      361 tttgtgcgat cactcgctgg caatcgtcaa tgtggatgcc aactgccata tccgactgca
      421 tcagcaagcg ctgccggatg gcgcacatat tttgattaat gacttccgta ttggcagtca
      481 ggttcaggac gatatttacc agctttggtt gcaaagccag cgccgattac tgccgatgct
      541 cattcatcgt gatgaagcga tggctgaatg cctggcagct aagcaaccag tcggtgaata
      601 tcgcagtgat gcgctggcgg ctgaagagat actgacgctg gcgaactggt gcctgttgaa
      661 ctattccggg ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac ccggtggttg
      721 cttatcccgc cggtcaacgc gcggcttatc gggcgttatc gcgattatcg tcgtcacggt
      781 gcgtcggctt tcagcgcgac gctcggctgt ttctggatga tcctggcctg gatttttatt
      841 ccgctggagc acccgcgctg gcagcgtatt cgcgcagaac ataaaaacct gtatccgcat
      901 atcaacgcct cgcgtccgcg tccgctggac ccggtccgtt atctcattca aacatgctgg
      961 ttactgatcg gtgcatcgcg caaagaaacg ccgaaaccgc gcaggcgggc attttcaggt
     1021 ctgcagaata ttcgtggacg ttaccatcaa tggatgaacg agctgcctga gcgcgttagc
     1081 cataaaacac agcatcttga tgagaaaaaa gagctcggtc atttgagtgc cggggcgcgg
     1141 cggttgatcc tcggtatcat cgtcaccttc tcgctgattc tggcgttaat ctgcgttact
     1201 cagccgttta acccgctggc gcagtttatc ttcctgatgc tgctgtgggg ggtagcgctg
     1261 atcgtacggc ggatgccggg gcgcttctcg gcgctaatgt tgattgtgct gtcgctgacc
     1321 gtttcttgcc gttatatctg gtggcgttac acctctacgc tgaactggga cgatccggtc
     1381 agcctggtgt gcgggcttat tctgctcttc gctgaaacgt acgcgtggat tgtgctggtg
     1441 ctcggctact tccaggtagt atggccgctg aatcgtcagc cggtgccatt gccgaaagat
     1501 atgtcgctgt ggccgtcggt ggatatcttt gtcccgactt acaacgaaga tctcaacgtg
     1561 gtgaaaaata ccatttacgc ctcgctgggt atcgactggc cgaaagacaa actgaacatc
     1621 tggatcctcg atgacggcgg cagggaagag tttcgccagt ttgcgcaaaa cgtgggggtg
     1681 aaatatatcg cccgcaccac tcatgaacat gcgaaagcgg gcaacatcaa caatgcgctg
     1741 aaatatgcca aaggcgagtt cgtgtcgatt ttcgactgcg accacgtacc aacgcgatcg
     1801 ttcctgcaaa tgaccatggg ctggttcctg aaagagaaac agctggcgat gatgcagacg
     1861 ccgcaccact tcttctcgcc ggacccgttt gaacgcaacc tggggcgttt tcgtaaaacg
     1921 ccgaacgaag gcacgctgtt ttatggtc
//
LOCUS       DAZUCE010000407         1946 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZUCE010000407 DAZUCE010000000
VERSION     DAZUCE010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.410"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>1946
                     /locus_tag="ACHY03_005114"
     CDS             <1..>1946
                     /locus_tag="ACHY03_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFS3908208.1"
                     /translation="IISTFHAEKIINDLLKNDYEISLFPDTTYRELNKAAQRSITAPD
                     RIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDYE
                     LAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEAI
                     LHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPEDL
                     CTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKIS
                     KDIESGIPGYWQGQVLIPFTIDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWENL
                     HALKEKRLNGMPKTTPKSNQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSIT
                     PPLKNYNERLHNTNLFNPKYHEKAVMNYMEYLVTCQLDEEQMSSLLYERDDLSAIDFS
                     LLMKTIKAFSFGGDLQTLASKPGSTISSIPSKRRILININHDFPNNGNLFNDFLFNHQ
                     QDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQL
                     YNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEG
                     AWPNKLQPGKYKNMGSNSVNIIIN"
BASE COUNT          681 a          378 c          363 g          524 t
ORIGIN      
        1 aattatatca acttttcacg cagaaaaaat cattaatgat ctgttaaaaa acgattatga
       61 aatttccctt tttccagata caacttaccg tgaactgaat aaagctgcac agcgtagtat
      121 tacagcgcca gacaggatag gagaagggaa aacatgggtt tatcaacgag atacaatggt
      181 tgaaagaggt gataacagcg gtgttcatca gtatggtcct gctgaacatt tcacccacat
      241 tatatctgac aaaccctccc caaaagataa atatgttgca tatgctatta acattcctga
      301 ctatgagctg gcagccgatg tatataatat taacgtgacg tcaccttccg gacagcaaga
      361 aacatttaaa atattaatca atccagaaca tctacggcaa acacttgagc gtaaatctct
      421 tactgctgtt cagaaatcac aatgtgaaat catcaccccc aaaaaacctg gcgaagcgat
      481 tcttcatgct tttaatgcca cctaccagca aatcagggaa aatatgtctg agtttgcacg
      541 ttgccattat gggtatatac aaatccctcc agtgacaact ttccgcgccg acggaccaga
      601 aactcccgaa gaagaaaaag gttactggtt tcacgcttat caacccgaag atctttgtac
      661 catccataat ccaatgggag atttgcagga ttttatcgca ttggttaaag atgctaaaaa
      721 atttggtatc gatatcattc ctgattatac ctttaacttt atgggaatcg ggggtagtgg
      781 taaaaatgac ctggattatc cctctgctga tatacgagcg aagatcagta aagatataga
      841 aagtggtatc cctggctatt ggcaaggtca ggttttgatt ccatttacta tagatccagt
      901 aacaaaagaa cgtaaacaaa tccatccaga agatatacat ctcactgcaa aagacttcga
      961 agcaagtaaa gataatattt ctaaggatga atgggaaaac ctccatgcat taaaagaaaa
     1021 gcgtttaaat ggaatgccta aaacaacccc caaaagtaac caggttatta tgctgcaaaa
     1081 tcaatacgtt cgtgaaatgc gaaaatatgg cgtacgaggt ttacgttacg atgcagcaaa
     1141 acactcaaaa catgaacaaa tagaaagatc aataacccca ccgcttaaaa attataatga
     1201 gcgattacac aatactaact tatttaaccc aaaatatcat gaaaaagccg ttatgaatta
     1261 catggaatat ctggtaactt gtcagttgga tgaagaacaa atgtcatcgc tgctttatga
     1321 aagagatgat ttaagcgcca ttgatttttc attgctcatg aagacgataa aagctttttc
     1381 atttggtggc gatctccaaa cccttgcatc aaaaccgggt tccacaatct caagcatccc
     1441 atcaaaaaga cggatattga ttaacattaa ccacgatttt cctaataatg gcaatctttt
     1501 caatgacttt ctatttaacc atcaacaaga tgaacaatta gcaatggcat atatggccgc
     1561 tctcccgttc agcaggcctt tagtttactg ggatggccaa gtattaaaat caacgactga
     1621 aattaaaaat tatgatgggt cgacgcgtgt cggcggtgag gcgtggctta ataaaggttg
     1681 ctctacctat cagcagctct acaatgaatt ccacgcatta tatatagata aagcaggaat
     1741 atggagtgca tttgagggtg tatttgcaac taaaaacgtt ctggccttta gtcgtgggga
     1801 ttctgtgaac attaatcact ctcctcatga tggactagtt ataataaata aaggaaacga
     1861 agaagttgaa ggtgcctggc ctaacaaatt gcaacctgga aaatataaaa acatggggag
     1921 taatagcgtt aacattatta ttaata
//
LOCUS       DAZUCE010000408         1905 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZUCE010000408 DAZUCE010000000
VERSION     DAZUCE010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.411"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..434
                     /locus_tag="ACHY03_005115"
     CDS             <1..434
                     /locus_tag="ACHY03_005115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFS3908209.1"
                     /translation="TPYCVMIMDIDHFKKVNDTWGHPIGDQVIKTVVNIIGKSIRPDD
                     LFARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVT
                     QGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
     gene            532..>1905
                     /locus_tag="ACHY03_005116"
     CDS             532..>1905
                     /locus_tag="ACHY03_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFS3908210.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSIFQMNRDTTITAGILLKQLDNVTQIVQHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMTAFSIVESRYIYDLMDVLDDENDDSFSLSFTEGPAI
                     ISGVNNNDKLYMLKKDFNSAISQASLTVTTPMISLYPYVISNVFYILPLSILLSFILY
                     FLWQRWISRKMSLAEEIKKGMSSGEFSVNYQPVCDTTTKACLGVEVLMRWQREDGKNI
                     SPVVFIRAAEEANLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIAAEHLAHPDFVAD
                     VLHIKNEISDKFNIVLEITERNLVEDTDLALQKINELRIHGCEFAVDDFGTGYCSLGL
                     LQKLSVDY"
BASE COUNT          574 a          326 c          414 g          591 t
ORIGIN      
        1 aaacgcctta ttgcgtcatg attatggata tcgaccactt caaaaaagtc aacgacacct
       61 gggggcatcc gattggcgat caggtgataa aaacagtggt gaatatcatt gggaaaagca
      121 tacggccaga tgaccttttt gcgcgcgtcg gcggcgaaga gtttggcgtc ttgctgacgg
      181 acatcgatac tgaacgtgcg aaagctttag cggaaaggat tcgggaaaat gttgagcgtt
      241 taactggtga taatcctgag tacgccatac cacaaaaagt gacgattagt attggcgctg
      301 ttgtgactca gggaaatgcg ttaaacccga atgagatcta tcgactggct gataacgcac
      361 tatacgaggc gaaagagacc gggcgtaata aggtggttgt gagggatgtg gtgaattttt
      421 gtgagtcacc ataaagcggc attttagcgg gtgattttgg taagcgctaa atagctattc
      481 gcagacatgc gtaacaattt tcagaaatat catagtacta tatgatctta aatgcttatc
      541 attgatcagt ctgctatcac ccgcttttac tggcaattgt actataattt cttaggttgc
      601 atcaccctga tgcgtgggga aacatgtatg gtattcaaca aaaaaatgtt tgtactcatt
      661 ataataccag gcattctggg ggtgttacta tcgtttgcta tgtcaatatt tcaaatgaat
      721 agagatacga caattaccgc cggtattctg ttaaaacagc ttgataatgt tacgcaaatt
      781 gtccagcata cgacaaagct tacctctatt ctggtgatga agccctgcaa agatatcctg
      841 gaacagttga ttgctaacgg agcattgact ccctatgttc gaacaacggg tcttatagaa
      901 aataatttcc aaatatgttc ttcagttagc ggtttcaaaa aaatgaatgt taacgatgtt
      961 tacggtacga gctttcacaa taaaaataaa gaatcacgca tagtatccat tagcggtaca
     1021 agttttgtac caggtaaaac agctatagta ttccttatgc caatagggaa tgatatgaca
     1081 gcttttagta ttgtagagtc acgatatatc tatgatctaa tggatgttct tgatgatgag
     1141 aatgatgatt cattttctct tagctttact gaagggcctg caattattag tggtgtaaat
     1201 aataatgaca agttatatat gttaaaaaaa gattttaatt ctgcgatcag tcaggctagt
     1261 cttacagtta ctacgccgat gatttcatta tatccctatg tcattagtaa tgtattttac
     1321 attcttccat tatctattct tctttctttt attctgtact tcttatggca gcgctggata
     1381 tctcggaaaa tgtccctggc agaagaaatt aagaaaggaa tgtcatccgg agaattttct
     1441 gtgaattatc aaccagtatg tgacacgaca accaaagcgt gtctgggggt tgaggtgtta
     1501 atgcgttggc aacgcgaaga tggtaaaaat atatctccgg tagtttttat ccgcgctgca
     1561 gaagaggcaa atttaattat accgttaact aagcatctct ttgaactgat tattcaggat
     1621 gtacaaagct ggaaagtgaa aaagccgttt caccttggga taaatattgc cgcggagcat
     1681 cttgcacatc ctgattttgt tgctgatgtt ttacatatta aaaatgaaat atctgataaa
     1741 tttaacattg ttcttgagat taccgaacgc aatctggttg aagatactga tcttgcatta
     1801 cagaaaataa atgaattaag gatccatggc tgtgaatttg cagttgatga ttttggcacg
     1861 ggatattgtt cccttggcct tctgcaaaag ttgtctgttg attat
//
LOCUS       DAZUCE010000409         1892 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZUCE010000409 DAZUCE010000000
VERSION     DAZUCE010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.412"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            191..>1892
                     /locus_tag="ACHY03_005117"
     CDS             191..>1892
                     /locus_tag="ACHY03_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFS3908211.1"
                     /translation="MKSVKLSLLAVVVATIVSPSAFADDTFEAAATELTAIHPGMSHD
                     EIDQKINRFMIRTGNTEAAQDYLFSHGYMDPQTGDNIYPASTAVQTDSNITDQNQADR
                     DAAQDTAIQNAHNIASIAAANADDAKKALTTAQSAIDANKGAIADTQKDVADVKASAA
                     HANTNANTALMNGLKLSGAVSDNKNQIEKNSSSIAEQQEQMALSEQNQIHRDAAQDSS
                     IQDAQHSANWASLKADDAHHAIMVAQTDIDANKSAISDTQKDVAAVRTDVSAMKDDVA
                     DVKASADHANANANTALMNGQKLSGVVSDNKNQIEKNSTAITEQQEQITATQKTVAAT
                     GDVKTAARYQSMINALQTATADAQQSQSEAQQTQITEQQKQIAATEKTVAAIGDPQST
                     AHYQKMINAKLTAQNEANERSTADQEQRINELASNVATQQQIDSVQYGEQIRNLAQDS
                     AQAHEQIESLTQDSAQTHQQLTNTQKRVADNSQQINSLNNNFSSLKHEVEDNRKEANA
                     GIASAVAIASQPQVKTGDFMMVSAGAGTFNNESAVSVGASFNAGIHTVIKAGVSADTQ
                     S"
BASE COUNT          621 a          460 c          418 g          393 t
ORIGIN      
        1 tatttactta agaaaccaat ttagtttctt atagacagtg tttacgctta cacaagacaa
       61 aaacgcgctt tacatcacac aaatggcggc gtagatttcg attaaattgc aacgcaaatt
      121 aactccaata gaaatattat tagtttctat tagaaaccaa aataaactta atttgatcga
      181 gaaaaaataa atgaaatctg taaagttgtc tttactggca gtcgttgttg ctaccattgt
      241 gagcccatct gcttttgcag atgatacgtt tgaagcggca gcgacagaac tgacagcgat
      301 tcacccaggt atgtctcatg atgaaatcga tcaaaaaatc aatcgcttca tgattcggac
      361 aggtaataca gaagccgcgc aagattacct gttttctcat ggatatatgg acccacagac
      421 aggcgataat atttaccctg ccagtacagc cgtacagact gacagcaaca tcaccgacca
      481 aaaccaggct gatcgcgacg cggcgcaaga taccgcaatt cagaacgcac acaatatcgc
      541 aagcattgcg gcggcaaatg cagatgacgc caaaaaagcg cttaccactg cgcaatctgc
      601 aattgacgcg aacaagggag ctattgctga tactcagaaa gatgtcgcag atgttaaagc
      661 aagcgccgct catgccaaca caaacgccaa taccgccctg atgaatggcc tgaaactctc
      721 cggtgccgtg agcgacaaca aaaaccagat tgaaaaaaac agctcctcta ttgctgaaca
      781 gcaggaacag atggcgttaa gtgagcagaa ccaaatacat cgcgatgccg cacaggactc
      841 gtcaattcag gatgcccagc actctgctaa ctgggcttcg cttaaagccg atgacgcgca
      901 ccatgccatt atggtggcgc aaacggatat cgacgccaat aagtcggcca tttccgacac
      961 acaaaaagat gttgccgctg tccgcaccga tgtgagtgca atgaaagatg atgtcgcaga
     1021 tgttaaagca agtgccgatc atgccaacgc gaatgctaat accgcgctga tgaatggcca
     1081 gaaactttcc ggtgtcgtca gcgacaacaa aaaccagatt gaaaaaaaca gtacggcgat
     1141 taccgaacag caggaacaaa tcactgccac gcaaaaaacc gttgccgcca cgggtgatgt
     1201 aaagaccgct gctcgttatc agagcatgat taacgctctt cagacagcca ctgctgatgc
     1261 gcaacaatca cagtctgaag cccaacaaac gcagattaca gagcaacaga agcagatcgc
     1321 agccacagaa aaaacggtcg ctgcaatcgg cgatccccag tcaactgcgc attatcaaaa
     1381 gatgattaat gccaaactga cggcgcaaaa tgaagccaat gaacgttcaa ccgctgatca
     1441 ggagcagaga attaatgaac tggcttccaa tgtcgcgacg caacagcaaa tcgatagcgt
     1501 gcaatacggt gaacaaattc gaaaccttgc gcaagactcg gcccaggcac atgagcaaat
     1561 cgaaagtctg acacaagatt ctgcccaaac gcaccagcag ttaacaaaca cgcaaaaacg
     1621 tgttgcagat aacagccagc aaatcaattc gctcaataac aacttcagtt cgttgaaaca
     1681 cgaagtggaa gataaccgta aagaggctaa tgcgggtatt gcgtcagccg tcgctattgc
     1741 ttcacagcct caagttaaga caggtgattt tatgatggtg tctgccgggg cgggtacatt
     1801 caacaatgaa tctgcggtat ctgtcggtgc ttcgtttaat gctggaattc atacggtaat
     1861 taaagcggga gtctctgcgg atacacaatc tg
//
LOCUS       DAZUCE010000410         1849 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZUCE010000410 DAZUCE010000000
VERSION     DAZUCE010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.413"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            7..1299
                     /gene="sctN"
                     /locus_tag="ACHY03_005118"
     CDS             7..1299
                     /gene="sctN"
                     /locus_tag="ACHY03_005118"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HFS3908212.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAILKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKQSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1289..1747
                     /locus_tag="ACHY03_005119"
     CDS             1289..1747
                     /locus_tag="ACHY03_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFS3908213.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYERLKTRIKKQLLDQMLYQDELEQEEKYNGRSQEN"
     gene            1725..>1849
                     /locus_tag="ACHY03_005120"
     CDS             1725..>1849
                     /locus_tag="ACHY03_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFS3908214.1"
                     /translation="MDEVKKIEHGKILNTDTPDNSASDLDSFLKKSKKNKEMSEIV"
BASE COUNT          612 a          331 c          444 g          462 t
ORIGIN      
        1 gccggtatga aaaaattaaa attattaaat aaatacagct acttacatag cataaacggc
       61 tctctgattg aagcagaact tgacgacgtt tctgtgggtg aagtatgcga aatttatgcg
      121 agctggcaag caaatgaacg tatagccagg gctcaggtcg ttggctttcg caatggaaag
      181 acattattaa accttatagg cagctcagtg gggcttacac gtactgcaat tttgaaacca
      241 acgggagagc agttgaccat acagattagt gatgcctttc ttggttcggt tttgaatgcc
      301 tctggtcaaa ttatggaaag gtttgtcccg gacactccag gcgatagagg aaatttacgc
      361 ctgattgatg aactgccacc gtcgtatcag gaacgtcgag ttattaatac accacttgag
      421 acacggataa gagtcattga tggcgtactg acatgtggca ttggtcaacg tgttgggatt
      481 ttcgcctcgg ctggctgtgg caaaacggtt ttaatgcata tgctcgtaaa caatactgaa
      541 gctgatgtgt ttgttattgg cttgattggc gaacgtggta gggaagttac ggaatgcgcg
      601 gaatcgctga aacaatcagt aaatgcagcg aaatgcgtat tggtatatgc cacctctgat
      661 ttttcgtcag tagaccggtg taatgccgcc ctgatggcga caaccgtggc agagtatttt
      721 agagatcgag gaaagagagt tgtacttttt atcgactcaa tgacgcggta tgcacgagca
      781 ctacgtgata tgaaactggc agcgggtgaa ccacctgcca gacgtggtta tcctgcttct
      841 gtttttgaca gtttgcctcg cttactggag aggccgggac caacactaaa aggaagtatc
      901 accgcgtttt ataccgtgtt actggaaggg gaagatgaat cagaccccct gggtgatgaa
      961 attcgctcaa tccttgatgg ccatatctat ctaagtcgca aactcgcggg tcaaggtcac
     1021 tatccggcaa tagatgttct aaaaagtgtg agtcgagtat ttggtcaggt cactgatgaa
     1081 aaacacagag ataatgctgc acgggtaagg aagatcctca caactttgga agatctacag
     1141 gtgtttattg acttagggga atatcgcgct gggcaaaatg ctgaaaatga ttttgctatg
     1201 aatgcacggc ctaaactcac aaattggttg aaacagtcag ttaatgaaaa aatgccaatg
     1261 agtgaaactc taaaagagct tgaacgaatt gttaagtaaa gttaatcgac ttataagaag
     1321 aacagcgcag agcctggctg catgtgaagc atcattacaa aagttaaatg cagaaaaaga
     1381 aaagttagcc gaaaaggaac gcctgtatga tatgcaactt aaaaacctgc agtcactgtt
     1441 agatatgaaa gagttactcg gcgaagttgt atttcgacag gatatatttt actcgttacg
     1501 taaagttgca gtcattcagc agcagatcgc tgagataaat cttgaaaagc aaaaaatagc
     1561 agagcggaga aaaatactta acaaggaaat agttcaacag caagcacaaa gaaaacactg
     1621 gtggctaaaa ggagaaaaat acgaacgact aaaaacgcgc atcaaaaaac aacttttaga
     1681 tcagatgctt tatcaagacg agcttgaaca ggaggaaaaa tataatggac gaagtcaaga
     1741 aaattgaaca cggtaaaatt ttaaacacag acacacccga taattctgct tcggatttag
     1801 acagtttctt aaagaaaagc aaaaaaaata aagaaatgag tgagattgt
//
LOCUS       DAZUCE010000411         1847 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZUCE010000411 DAZUCE010000000
VERSION     DAZUCE010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.414"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1664)
                     /gene="rhsD"
                     /locus_tag="ACHY03_005121"
     CDS             complement(<1..1664)
                     /gene="rhsD"
                     /locus_tag="ACHY03_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFS3908215.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGIFGPGWKAPSDI
                     RLQLHDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKATQPDGHTLARLWA
                     SLPPDIRLSPHRYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYLYEQDRIT
                     VTDSLNRREVLHTEGEGGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRRAYDELDRLVTETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTR"
BASE COUNT          339 a          622 c          502 g          384 t
ORIGIN      
        1 cgggtctggt agcccgagca gtcggtgaac gccagcaact gaccgtagcg gctccacgtc
       61 atctgccggg tgctgcccgt cgcatctgtt gttgtggccg gtaattcact gtgcgcatca
      121 tcatagcggt agcgtactgt ctccccgctg cgcgatgtct ccgttaccag cctgtccagt
      181 tcatcatatg cccggcggct ctccagcccg tccggggaca ccaccgccgt cagctggttc
      241 ccgtcgttat agtaaaattt cgtctcccgc ccgtccggtg tggtgatgtc cgtgatatcg
      301 ccggacacca cattcagacc gtactctgtc cgccgtcccg ccgcgtccgt ctgcgccgtg
      361 agccttcctg ccgcgtcata cccgctgtgc gtgacgctgc catccgccag ttcttttttc
      421 accacccgtt tcagcccgcc ttcaccttct gtatgcagca cctcacgccg gttcaggctg
      481 tcggtgacgg tgatgcggtc ctgctcataa agatagcggt aacttaaccc tgcagggttc
      541 agttgctcca ccacccgccc cgcatcgtcg tagcggtagc gcatctccgg ccttcccgca
      601 taacggtgcg ccaccatccg gcccgggtgc tgcgcgtcat acgtgaaagc gcgcacctgc
      661 gtattgctgc ggtcatatac cgccagcagt tcaccggctt ccgtatacgt gtaccgcacc
      721 agtggcgcag cgggcaggct ctccgggtat gccgggtcgt gcatcagcca caccgccgaa
      781 aggcggatac ccctgtcggg gccgtattcg gtaccgggca gtgtgtcggg gaacgctgag
      841 gctgagagag ggcgggaact gtcagaagaa gatagcgaag aggtgcgggc ctcttccgca
      901 cgctgcgcct gcgtggtcag caccagacgg aactcccgcc cggcaccgtc cgtcacgccg
      961 gtgatttccc cggtcaggtc accggcggcc tcacgccggt acgtcagcgt ccgcccgaag
     1021 cggtccgcca gccccgtcag ctcccggtac ggcggcagcg gcgctggcag tacgtcctca
     1081 gcacccggca cccgctcaga ccaccccagt atccaccacg gcccctgtgc gctgttggtc
     1141 gccaggtaac gatgcgggct taaccggata tccggcggca gcgacgccca cagccgtgcc
     1201 agcgtatggc cgtccggctg cgttgcctta ccaccgcgca ccagccacat tgactcgctg
     1261 cggctgtaca ccgcctcccc cggcagcagc ggctcaaagt gaatgctccg cccgccgttg
     1321 tcgttgagta tcagtccgtc atcatgtagc tgtaagcgga tatcagaagg cgctttccag
     1381 ccggggccaa aaatccccac cggcgcaggc gtcttcgtcc ggtagctgct gtaggtgcgg
     1441 gagagaatga acggcagcgg gccgggcagc gcaaggtccg tctcgccggg cagcaccttc
     1501 gcccccagca gcggatttac cgggttgccc gaagtcatcc cgcccggaca caccgagcac
     1561 gccacgccag ttggcgcgcc aattcttaca cctgccgaac cctggacaat gggaccgcca
     1621 tactgagtca tatctccctg acgcgccgct ggttttccgc tcatataaag tgccctttta
     1681 gttattgttc atatccctgt gttagcaaca aaaaaaataa aatgacacac aagaccagct
     1741 aatgtattta tatatttttc acataaactt atttgttctc aatcaacaac aactgttatt
     1801 acaggtcact ttcaatattt tattaaacta aaatttttag cacagat
//
LOCUS       DAZUCE010000412         1814 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZUCE010000412 DAZUCE010000000
VERSION     DAZUCE010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.415"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            119..>1814
                     /locus_tag="ACHY03_005122"
     CDS             119..>1814
                     /locus_tag="ACHY03_005122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFS3908216.1"
                     /translation="MTIDGTTLKNEIKNDAIKIKGGDGKTLTKSLGDNVEITGKGPIK
                     TQVAGDKLEISAETASLPVNGGNGKITVNNGDGDKLVTAKNIADAINQSGWKIKADKD
                     TGGVIEGNGQGTETLIKPGDMVTLKAGKNLSVKQSNGDFTFSVIDTPSFSSVQLNGNG
                     KNGNVTLSSEGNKLTLSGGNGAGQNGVVISGVASGLNNKDLAGLKADDAEMKNAASVA
                     DLKTAIDGVSTSMQGAGFALKDSKGQEVKQSLGQAIKVSGDSNITTTAITANGDKGLK
                     ISLNNDLTVGDKAPGSIKVNGADGKEAVSLNGQDGTIKLTSSPAAGGKSASATIAVNA
                     GKGGLTDAAGTTKTRLTYTPDGKSPAEELATMNDGLSFEGDDGKVIHKKLNEKLTIKG
                     GAEKNAEVTDGNIRVDNDNGGLLVRMTRNLRDLASAVFGTENGPTTRIDNAGITITTP
                     AAGGGQGNTVSLTGSGLNNGGNQITSVASGLNGTALADASGDVLNNAANIGDLKNALR
                     DSSAALVNTGFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLTVRLADNIRL"
BASE COUNT          554 a          343 c          521 g          396 t
ORIGIN      
        1 gctaaaggat gataagggag gttctgcgac tattaatggt gggaaggata ataagaatgg
       61 taacacttta actgttaaag gtgatggaga tattgcagtt actgttaatg ataaaggaat
      121 gacgattgac ggaacaacgc tgaaaaatga aataaaaaat gatgccatca aaattaaagg
      181 tggagatggt aagacgctga cgaagagtct tggtgacaat gtcgaaatca ctggcaaagg
      241 cccaataaaa acccaggtgg ctggagataa actggagatt tcagcagaga cagcttcgtt
      301 gcctgtaaat ggtggtaacg ggaagattac agtaaacaat ggtgatggcg ataaacttgt
      361 tactgcgaaa aacattgctg atgccataaa ccagtctggc tggaaaatta aagcggataa
      421 agataccggt ggtgttatcg agggaaatgg tcagggaaca gaaacactga taaaacccgg
      481 tgatatggtt acgctgaaag ccggtaaaaa tctcagcgtg aaacaaagta acggtgattt
      541 tacattctct gttattgata ctccgtcatt cagcagtgtt cagctgaacg gtaatggaaa
      601 aaacggaaat gtaacgctct catccgaagg taataagctg acattgtcag gcggtaatgg
      661 tgccggacaa aatggtgttg ttatcagtgg tgtggcaagt ggattgaata ataaggacct
      721 ggcaggcctg aaagcagacg atgcggaaat gaaaaatgcc gccagtgttg cagacctgaa
      781 aacggcaatt gatggtgtgt ccaccagtat gcagggtgca ggtttcgcac tgaaagattc
      841 taagggacag gaagtgaaac agtctcttgg gcaggcgatt aaagtatccg gggacagcaa
      901 tatcaccacc acagcaatca ctgccaatgg agataagggg ttaaaaatat ccctgaataa
      961 tgacctgact gtgggggata aggctcccgg cagcatcaaa gttaacgggg ctgatggtaa
     1021 ggaggctgtg agtctgaatg gtcaggatgg cacgattaaa ctgacatcct cgcctgctgc
     1081 gggtggaaag tctgcgtcag caaccattgc tgttaatgca ggcaaaggtg gattaacaga
     1141 tgctgccggt accacgaaga cacgtctgac ttataccccg gatggaaaat caccggcaga
     1201 agagctggcg accatgaatg acggtctttc ttttgagggt gatgacggaa aggttattca
     1261 taaaaaactg aatgaaaaac tgaccatcaa aggtggtgca gagaagaatg ctgaagtgac
     1321 tgacggcaat atccgggttg ataatgataa tggcgggctt ctggtgagga tgaccagaaa
     1381 cctccgtgat ttggccagcg cggtttttgg tacagaaaac ggcccgacaa cccggattga
     1441 caatgcaggc atcacaatca caacacctgc tgcgggcggt ggtcagggga atacggtttc
     1501 ccttactggc tccggcctga ataacggtgg aaatcagata accagtgtgg ccagtggtct
     1561 gaacggcact gcgcttgctg acgccagtgg agatgttctg aataatgcgg ccaatatcgg
     1621 tgacctgaaa aatgctctcc gtgacagcag tgcggcactg gtgaataccg gctttggtct
     1681 gcaggcggat gacggaagca aggtgaacac gtctctcggg aagcatatca cggttggtgg
     1741 tgacgggcag aatatcagca cgactgttca gaacgggagc ctgacggtca gactcgctga
     1801 taacattcgc ctgg
//
LOCUS       DAZUCE010000413         1805 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZUCE010000413 DAZUCE010000000
VERSION     DAZUCE010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.416"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            823..1047
                     /gene="hha"
                     /locus_tag="ACHY03_005123"
     CDS             823..1047
                     /gene="hha"
                     /locus_tag="ACHY03_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFS3908217.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVITRTRYKLTPAELEA
                     FNSAVDHRLAELTMNKLYDRVPASVWKYVT"
     gene            complement(1379..1576)
                     /locus_tag="ACHY03_005124"
     CDS             complement(1379..1576)
                     /locus_tag="ACHY03_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908218.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGAVDPFGYYRKTDRLLCLL
                     TLLLVLILVTVSGVSVFVLLQ"
BASE COUNT          481 a          419 c          478 g          427 t
ORIGIN      
        1 ggcgttggtg atgaaaacaa cgcaccacct tcaccggcat aacaaccggg gagaaacgta
       61 aaaaataatc gggaatgtga ccgtcaggag ataaacgggg ggattgcggt ggctgagccg
      121 taccggtatg tcgggtgatt gttgcgaaac gatgcttccg cacgcgcacg ggaggacttt
      181 cgtgagacaa ggggaaaact ggcgttctga taattgcaga gttgtttttg aaggcggttg
      241 tggtgcatca gcctgtgacc tgtgggcagg cgacaggtat cactggtggg ataagactgt
      301 ctgattggta ttataaatat ataagagagc tcaaacacat gagttcatat atgtgaaata
      361 aatctgtgag tgtgatcagc agaccagtct acccagtagc aacagattgc gttgtactca
      421 cagggttatc cctgataact ggcgtataac ctgcgtacat aaaacgtacc tgcaatgcac
      481 ctgaacagag tgcattaagc atccgcctga gcggtaaacg tatccgatct ggtgagtgac
      541 agtctgagag cattctcagt aatcgccccc ttgtatcctg gaaattggtt aagagcgatt
      601 agatttggca aggtgtatgc atccagcagc ttaacacctc agctaactgg ctggttacct
      661 tcaggttgtc gcaatggcat cggacatccg gtgtcggata actaaacgta gcgttaaggc
      721 gatattgctg agagaccggt taagtggtgg cacgggagta atgagtttgg ggagggtacc
      781 agaatgccag tcaggtgcgg tatgctggca gtatcacgtt gattgcagca taaagtaacg
      841 agagacgaat ggaagttaaa accagagcag ttccgtcgtt gttcatcccg ggagacgctg
      901 gaaaaagtga ttacccgcac acgttataaa cttacccctg cggagctgga agcctttaac
      961 tctgcggtcg atcaccggct ggcagaactg acaatgaaca aactttacga tcgcgtgccg
     1021 gcttccgtct ggaaatatgt cacctgaatc ttgaatccgc gctacgatga ctctggtgtt
     1081 atctgtgcac cggaagatcg ggaacattaa gcagtataac ggcaacaaag aattcgaaat
     1141 caaaagggaa tggtgtacag aggggcaggc gttcaggcat taccggcctg tatcttctga
     1201 aggggactcg tatttgtcgg ggctgagtat tccgctcgat tcgatcacgg attggcagaa
     1261 acctgactgt gattgattca ggtaccattt tgcagggatc cggtgcgtta ctgtctggca
     1321 caacaaacgc agcgggtcgc tctgccccgc cgattcctga taattcctcc cctgtaacct
     1381 actgcaacag tacaaataca ctgacgcctg aaacggtaac cagaatcagc accagcagta
     1441 acgtcagtaa acagagcaga cgatcggtct tccggtaata accgaagggg tcgacagcgc
     1501 cacaatacgg gcaacacact gtattatgag taattgcgtt tccacagtcc ctgcacttct
     1561 ttttgcgtat cacgatcgct ccctctccac gccttatccg taacctgttt tttacattaa
     1621 aaaaacaacg ccgggaattc aatcgtcagt ccggagacgg ccgttcgggt tatcacagag
     1681 tgcctgagac agtgtcctgc cggaggtcac ctcaggggct ctgcgcattt tttacgacgt
     1741 ggctattccg tatgaaccat acggagattt aaccatgacc tacaaataca accccttctg
     1801 gcagc
//
LOCUS       DAZUCE010000414         1804 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZUCE010000414 DAZUCE010000000
VERSION     DAZUCE010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.417"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(101..1204)
                     /gene="zorE"
                     /locus_tag="ACHY03_005125"
     CDS             complement(101..1204)
                     /gene="zorE"
                     /locus_tag="ACHY03_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorE"
                     /protein_id="HFS3908219.1"
                     /translation="MKLSIDLSELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLQNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPATL"
     gene            complement(1208..1803)
                     /gene="zorB2"
                     /locus_tag="ACHY03_005126"
                     /pseudo
     CDS             complement(1208..1803)
                     /gene="zorB2"
                     /locus_tag="ACHY03_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001450770.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II Zorya anti-phage system protein ZorB2"
BASE COUNT          518 a          364 c          328 g          594 t
ORIGIN      
        1 gacaagttat gccataaact acttttgtaa tttgagtcac aaatcaggga agttttattt
       61 atcttccgtg aaagtcttaa ctttcacgga gtgttagaat ttacaaagtt gctggcgtaa
      121 aggctccggt ttgtatttct ttaaccagct cacccacggt atatattctc catccttcga
      181 cttcggcggg agtaaggtta actgcaattt taaggcctgg ccatgcagct tctacggttg
      241 caataatttc atgttctgag ttttgaagat ctaacccagg aacaggaggt tgaaagcctt
      301 tatgttgcat catattaata tcaccatgca ctgatggatc agtcaggtca tatatttcat
      361 tccaggattc tgcttttaat aacccttgtt gggaacgtaa acgttgaatg atagccatct
      421 cagcttgggt aacgaatata ccttcgtgga ggggctgttt tcgatggcaa tccttgcaca
      481 gaacaagaag gttttcatgg tgattatcat atttgatgcc atttttatga tggacatggc
      541 acaagttttt ggcggttgat aaattcacgc cacaatcatt acaaacataa ttggcctttt
      601 ctcgtatttc tttagatatt tccttccagt tttcgacata cccactattt gttttgtcat
      661 agataccctt aggcatatga cgaaaacagg tactataatc tgaaaaaaac tcatttaatg
      721 agaatgattt aaaaatctct ctttttcgtt gtttatcaat acttccttta taattaagtc
      781 gttcgaggca attcatacat acccgtaaat caacatcctg gctccgacct gaaccatcat
      841 caatttcgaa taaaccatct atgcggttag ttgcatgata cctttcaaat cgatttttat
      901 ggcgcatttc atcaagagtt ctgcaccagg caatatgaaa acgttttcct ttttcgccat
      961 ctacgatagc cgcatcataa cgccctgaat ggtcccgaat atataaaaga acctggcggc
     1021 catgataaga gataagcccg ctaccagggt caagatcttc gatactaact tctttacccg
     1081 tggataactc aatatctata ggatcaaagt caatagggga ttcatccaga aaaaaatcga
     1141 ctccttctgg taacattttc ttccctaatt gaataagttc tgaaaggtcg atagataatt
     1201 tcatctctta ctcctgaata atctttctaa tctgcaactc ggcatttgtt acaactttga
     1261 aggtgacacg acgagagcga ttaggatcct ccttgccggt tttatcaaga atgggatgtg
     1321 cagatgaata acctactgcg gcaaatttac ttttaaccca ttgttggtgt gtcgcgatat
     1381 tttttatgtc ataaacgtat tgtaatactg cacgtgtacg accttgcgat agtgccatat
     1441 tattaaaata agcaatatca ggattcgttg ttcctgtcca gtcagtactt gtgtgacctt
     1501 caatgcggac ttcagtaata tgttcctgat aattatctag aacttttagg tagcgaggaa
     1561 agaagttgtc aagaatgagt ttaaactttg gttttagctc tgtgcttcct aagccaaata
     1621 aaacatccgg tgatttaaat cgaacctcca gagtctgttt atcgatctct gcatcccagt
     1681 cttgtaaatc ctttgcaaac tcaatatcca gagcattata aagctgtaac tgagcattct
     1741 cgtaggctac ggcaacttct ttaatttttc tttttcaata cgtacgtagt gcatataagc
     1801 aata
//
LOCUS       DAZUCE010000415         1800 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZUCE010000415 DAZUCE010000000
VERSION     DAZUCE010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.418"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            38..1285
                     /locus_tag="ACHY03_005127"
     CDS             38..1285
                     /locus_tag="ACHY03_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFS3908220.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            1571..>1800
                     /locus_tag="ACHY03_005128"
     CDS             1571..>1800
                     /locus_tag="ACHY03_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908221.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQP"
BASE COUNT          524 a          337 c          519 g          420 t
ORIGIN      
        1 gagcagatat gtttaacagt ttgtggaccg cagggatatg acagaaaaaa cggaacggaa
       61 taaagcgtcg tcagaggtgg tcaggcagtc agacctggcg ctgttgacag atgtacaggc
      121 agcccttcag caggaaaagc atcatgccct tttcctgatg gtgatttttc tgctggtact
      181 ggtagtgacg tttgttatct gggcatggaa cagtcctctg gatgaagtga cccgaggtca
      241 ggggagtatt attcccggca gcagggagca ggttatacag acccttgacc cgggtatact
      301 gaaatcactt gaggtcaggg aaggcgatat tgttgagaaa ggtcaggtac tgctgacact
      361 ggatgatacc cggagctctg ccatgctgag ggaaagtgaa gcccgggtga ataacctgga
      421 ggccgtcagg gcaagactcc gtgcagaagc ttattccgaa tcactgacat ttccggataa
      481 cgttcctgag gatttgcggg agcgggaaag tacggtctat tttctccgga agacggagct
      541 ggcgcagagc attgccggtc tgaagcagag taaggcgctg ctggacaggg aaatagcaat
      601 gacgcggcca attgtgcgtg aaggggcaat gtcggaggtc gaactgttac gtatgcagcg
      661 tcagtcagcg gagctgcagt tacagatgga tgagaaacag aataaatacc tgacggaagc
      721 cggtgctgaa ctggtgaaga cagaagcaga gcttgctcag gcaaaagaga atatggctgg
      781 cagagccgac ccggttgagc gttcacgtct cagggccccc ctgagaggta tcgtaaaaaa
      841 tatacgggta aacacactgg gaggggtggt ttctgcagga caggacataa tggaaattat
      901 tccgctggaa gaccagttgc tgattgaagc ttatataaat ccacgggatg ttgcctatgt
      961 cagaacaggg atgccggcac ttgtaaaact gacggcttat gactatgcca tttatggtgg
     1021 tcttgacggc gtggtgacac tggtaagtcc ggatacttta cgtgaccaga aacggccggg
     1081 agatttaaaa ctggacccga atgaagctta ttaccgggta ctggttacca ccagtaataa
     1141 ttatctgaca gacaggaacg gaaaaagatt gccagttatt cctggcatga tagccagcgt
     1201 ggatataaaa accgggcgta aaacggtatt tcagtatctg attaaaccga taaccagaat
     1261 gaagcaggca cttcaggaaa gataataaaa aggtcttccg gggaaagtat tttaaatcaa
     1321 ttttaaacgg gcagaatgat ttcactttat tctgcccggg tcattgcgca tgcctgtatg
     1381 tgatttgggg ctgaagataa tataaatgtt atatctgtca ggggaaaata aatctgaaaa
     1441 aagcggaatg attaaggagg tccggtcaga gaaataaatt atctgtaatc tgattcgttt
     1501 ttatttaatg actcccttta gcggataaaa cataagagca tactggtttt attaacctga
     1561 ggatacttat atgaaaatga ttttcaccgg caaggtcagt ggagaaaaaa cagtactgac
     1621 agccggagcc cgtcatacag taaaggcaca ggccggggaa cagtatggac tggttgatga
     1681 ggtgacaggt ctggtcccgg atggtgtgga agcggaccgt tctggtgatg atttaattct
     1741 gcgtaaaaag gaagatgata cagaaatccg gattgaggga ttctgggaag aatgccagcc
//
LOCUS       DAZUCE010000416         1798 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZUCE010000416 DAZUCE010000000
VERSION     DAZUCE010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.419"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            61..936
                     /locus_tag="ACHY03_005129"
     CDS             61..936
                     /locus_tag="ACHY03_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFS3908222.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLAAGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            944..1720
                     /locus_tag="ACHY03_005130"
     CDS             944..1720
                     /locus_tag="ACHY03_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFS3908223.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKRKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVSFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          517 a          407 c          416 g          458 t
ORIGIN      
        1 tttatcataa ttaatgtttt gtcaataaat atatggaaac atattgtttc agatgggaat
       61 atgagaaagt tcatccttat ttctatgata acgttagcta gtgttagtct gggagcctgc
      121 agacagaatg tcaccataaa gcaggatgcg ccagggcaga aagctttcct gactgacggg
      181 cagatttacg atttacacag tggcaaaatt atttctagca gcgagctttt ggcggatctt
      241 gctaccgctc gacaccttat tattggtgaa aaacacgata atgccgagca tcatcagatt
      301 gagctttggc ttattcaaaa tctgctcatc cagcgaccac aggggagtgt gttgcttgag
      361 atgttgacgt cagagcaaca gccccgagtt aaccaagtta aatgttggct gaaagataat
      421 cctgtggttc gagatagccg tgttcaggaa ctacttaact ggcaaaaagg gtggtcctgg
      481 gagatgtatg gcgacatcgt catgcagcta ttacgtgggc cttacccgct gctcaatgcc
      541 aacattggcc gggagcagat gttggcactg tacaaaaaaa acgaatttcc taaaggaaaa
      601 aaatccactg cacctgtggt acaagaagct ctgcgtgaaa ccatcatagc tatgcatgaa
      661 ggcaaccttg aaagccagca actgacgtca atgctctctg ttcagcagca gcgggatcgc
      721 tacatggccc gccagcttct gagtgccccg gtaccgtcgt tgttgattgc aggtggttat
      781 cacgcgtcaa aaagcatggg cgtaccgtta cacatggaag atcttgccgc cggcacccat
      841 ccagtggtgt tgatgcttgc agaaaaagga atgaacatca cagtagatca tgctgactac
      901 gtgtggtttg tagcgccaga tacaacaaaa aggtaaacca tggatgatga atattctcca
      961 cttcccacag tcggtaaaat ggagcagctg gtttatctgt tctctactgc ttcatggact
     1021 gattttcctt gcgtttatct ggcgtttttc cgaagttcag cccgctatgt ctcctgcacc
     1081 ggcgatcatg ctgcaatggg cagaaattat agaggctcct tcttctccac tttcgcttcc
     1141 ggtgggaatc gctcaacagg agtccgcagt gacagaagaa aagcaacaaa ctgaggacag
     1201 acaacaacgt cctgtcactg aagacagtga tgcaaccatt gaaataacca ggaagaaaaa
     1261 gtcatccgat ggagaaaaga gaaagacacg tccacctcgt aagataaagg ctcaaaccag
     1321 cgacagcaac cccacagccg tttccagtaa tgctgcacct caggctctgg tcgaatcgtc
     1381 ccgtattgct gcgcctttta acagtgactc aacaaagcga gataattctg aggcatcttg
     1441 ggaaagcaga gtaaaaggcc accttaaccg ttataaacgt taccctggcg atgccagaaa
     1501 acgtgcacga accggcacag ccgtcgtgag ttttaccgta aacacggaag gcactattgt
     1561 cagtagtttc ttggaaatct cttcaggaac cctttctctt gaccgcgaag ctattgctgt
     1621 tttagaacgc gctcaaccac ttcccaaacc accgcctgaa atattggagg ggggattatt
     1681 taaagtaaaa atgccgatta cctttaagtt gaaggagtaa agggataatt ggccgttcaa
     1741 gttcatttaa ctgaaattta attttcattt ttatgctgaa cattttatca aacttttc
//
LOCUS       DAZUCE010000417         1795 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZUCE010000417 DAZUCE010000000
VERSION     DAZUCE010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.420"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..99
                     /locus_tag="ACHY03_005131"
     CDS             <1..99
                     /locus_tag="ACHY03_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013035836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFS3908224.1"
                     /translation="MCKGVTKHPDGYVYYGDTPDFIKGKIYPYGNW"
     gene            130..396
                     /locus_tag="ACHY03_005132"
     CDS             130..396
                     /locus_tag="ACHY03_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFS3908225.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            400..1548
                     /locus_tag="ACHY03_005133"
     CDS             400..1548
                     /locus_tag="ACHY03_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFS3908226.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFFKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREITDK
                     FCQLPVVLPVNVTLITDQPDSEIRSAFVSAWEVLFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCLGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            1541..>1795
                     /locus_tag="ACHY03_005134"
     CDS             1541..>1795
                     /locus_tag="ACHY03_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908227.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDD"
BASE COUNT          444 a          341 c          461 g          549 t
ORIGIN      
        1 atgtgtaagg gggttactaa acatccagat ggatatgttt attacggtga tacaccagat
       61 tttatcaaag ggaaaattta cccctacggt aactggtgac tcaaagtaaa atttattttt
      121 gagagtttta tgtctaaagg ttttgtattg cttggtgata ataccacaca tggtggtaaa
      181 gttatttcgg catcttcaac catgattgtc gatggtaaaa aagtagctct tgtcggagat
      241 atggttagtt gtcctgtttc cggacatggt actaatccta ttgtggaggg ctctcctgaa
      301 tggatatctg atggaagagc tgttgtgatt gatggatgta aatgtctgtg tgggtgtaag
      361 gttatatcca gtgcaccgga atgtgcaata ggataaccca tgggatggag cagaacaaaa
      421 tcattatcaa cagaattacc tcataaacca tcaatgatgt tgtggtttat gacaggggca
      481 tttatggctg ttgttggtgc tctgttgttt attatccggg catcagaata tgtgaaagca
      541 ttaaatgatt tcagtatctg gtggcttgca ttgactcctc cggggggctg gtttttcctg
      601 ttttgcctgc gacactggca gtggagcaac cagatggatg aacatttgtt ctttaaaaaa
      661 gagggggagt atgctcagaa acagtgggaa tcctgggccg aaaggtatct tgttattact
      721 gccagttgtg tttatttgcc tgataaaata acggtagcta ctctttgtga tgaattgcct
      781 ctgcagtacg gccttgtaaa gaaaatagat tatctttctg acagtgggca taaggttgaa
      841 gcctctttac gtgtcttgct tagggagata acggataaat tttgtcagtt acctgtcgtt
      901 ctccctgtaa atgtgacgct gataaccgat cagcctgatt ctgaaatcag atctgctttt
      961 gtttctgcat gggaagtgtt atttccccaa cgtgttgttc ctgacaatat cgaggttact
     1021 cccgactttt ccatggggtg ggttgatgag cgtctgaaac aaccagtgct gactgtggat
     1081 ttgatactgg taatacagct taatggggga aatgcatact ctgacggact ggctgcactt
     1141 ttgttgacca gcgatgatgt tgcgcaaaaa tataacctgc cgcatccagc ccgtctgcta
     1201 cgcccgatgt cgctggatat caacaagttt aatgatgaat tcacactttt ccttgaaacc
     1261 cagacagctg cctgtcgtac cgctcgtgta ctgggggact gttatcactg ggaaaagatt
     1321 gctgcaccgc tgatgactat tggaaaccag tatggtgccg gatgggaacc gtccggacgt
     1381 atgttactgg aaaaatggtg cggtatcccg ggacctgctg ctccgtggct gcttactgct
     1441 ctggcagccg atctggtctg tcttggtaat gattctctcc tgactttatt ctcatccgga
     1501 gaagaacatt ttatcagtac tgttacatca ggaaaccaga atgaataaac tggcctgtct
     1561 gtctggtcgc tttggacgac cggggattgt atttattggt gtggctgcgc tctggtggct
     1621 gataacccga tatggagcat ttctgggggc agaaacacgc cgggatcaaa tactactgct
     1681 gatattgctg agtctggggt tgctctttgt ctgttacctg cctgtgatga aaaaatatgt
     1741 tcaggaactg acatatcgac gtcgggcccg taaggagcaa aggttacctg acgat
//
LOCUS       DAZUCE010000418         1757 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZUCE010000418 DAZUCE010000000
VERSION     DAZUCE010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.421"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            135..350
                     /gene="essD"
                     /locus_tag="ACHY03_005135"
     CDS             135..350
                     /gene="essD"
                     /locus_tag="ACHY03_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFS3908228.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            355..666
                     /locus_tag="ACHY03_005136"
     CDS             355..666
                     /locus_tag="ACHY03_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFS3908229.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            663..1196
                     /locus_tag="ACHY03_005137"
     CDS             663..1196
                     /locus_tag="ACHY03_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFS3908230.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1193..1690
                     /locus_tag="ACHY03_005138"
     CDS             1193..1690
                     /locus_tag="ACHY03_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFS3908231.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQM"
BASE COUNT          510 a          360 c          448 g          439 t
ORIGIN      
        1 caaaataatt acccacattt ttaatgtgta tgttttttta gcgccgttga gaacaacgtg
       61 tgctgtcaaa actaccccgt agactccgat cttttcaaac atattgcacc atccatgtac
      121 atcggggtga ggatatgaaa tcaatggata agttaacaac aggtgttgcc tatggcacat
      181 cggcgggtaa tgctggtttc tgggcattgc agttactcga taaagtaact ccgtcacagt
      241 gggctgcaat cggtgtgctg ggtagcctgg tttttggcct gctgacgtat ctgacaaatc
      301 tttatttcaa gattaaagaa gacaggcgta aggctgcgag aggagagtaa tccaatgact
      361 caagactatg aactggttgt gaaaggagtc cgtaattttg agaataaagt tacggtaact
      421 gtagccttac aggacaaaga acgctttgac ggtgaaattt ttgacctgga tgtcgccatg
      481 gaccgtgttg aaggagctgc gctggagttt tatgaggcag cagccagaag gagcgtccgg
      541 caagtcttcc tggaagtagc agaaaaattg tcagaaaaag ttgagtctta tctgcagcat
      601 cagtactcct ttaagattga aaatcctgcc aataagcacg agcgtcctca tcataaatat
      661 ctatgaacac aaaaatcaga tacggcctgt cggctgccgt tctggcgctg attggtgctg
      721 gcgcatctgc tcctcagata cttgaccagt ttctggacga aaaagaaggc aaccacacaa
      781 tggcataccg cgatggttct ggcatatgga ccatctgtcg gggtgccaca gtggtggatg
      841 gaaaaaccgt ttttcccaat atgaaactgt cgaaggaaaa atgcgaccag gtcaacgcca
      901 ttgagcgtga taaggcgctg gcatgggtgg agcgcaatat taaagtacca ctgaccgaac
      961 cacaaaaagc gggtatcgcg tcattttgtc cctataacat tggccccggt aagtgtttcc
     1021 cgtcgacgtt ttataagcgg ctgaatgctg gtgatcgtaa aggtgcatgc gaagcgattc
     1081 gctggtggat taaggatggc ggacgcgatt gccgcattcg ttcaaataac tgttacggtc
     1141 aggttattcg tcgtgaccag gagagcgcat taacctgctg ggggatagaa cagtgaatca
     1201 gatattcatg gtgatttttc tcgtgttgtc aggatttatc gtcggaaatg tctggagcga
     1261 ccgaggatgg caaaaaaaat gggcggaacg tgatgctgcc gcattatcac aagaggtaaa
     1321 tgctcaattt gctgctcgaa taattgaaca ggggcgaact atagcccgtg atgaggctgt
     1381 taaagatgcg caacagaaat ctgctgaaat ttctgccagg gctgcttatc tgtctgatag
     1441 tgttaaccag ttgcgtgccg aagcaaaaaa atatgccata cgccttgacg cagcgaagca
     1501 taccgcagat cttgccgctg ccgtcagagg caaaacaacc aaaaccgccg aaggaatgct
     1561 caccaacatg ctcggagata ttgcagcaga agctcagctt tatgctgaaa ttgctgacga
     1621 acgctacatc gcaggagtga cttgtcaaca gatctatgaa tctttaagag ataaaaagca
     1681 tcaaatgtag ggtaatatta aatcggaaca tttacatcgc ggaatgtaaa atttaaataa
     1741 aaaggactct tccatga
//
LOCUS       DAZUCE010000419         1735 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZUCE010000419 DAZUCE010000000
VERSION     DAZUCE010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.422"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            57..800
                     /locus_tag="ACHY03_005139"
     CDS             57..800
                     /locus_tag="ACHY03_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS3908232.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVISTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFVREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSCAQAGDILLCCFGSSVPNHAAIYCGNGDLLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASVCM"
     gene            797..1378
                     /locus_tag="ACHY03_005140"
     CDS             797..1378
                     /locus_tag="ACHY03_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS3908233.1"
                     /translation="MNTAAEAIRALSQQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRSVIHIVPRLAGAGGNGVFQVVLGAAAIVGSFFTAGATMALWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          386 a          412 c          546 g          391 t
ORIGIN      
        1 ggtcaatttt ggcggtttcc tttccattaa taaactttcg cagtaaatcc cgttttatga
       61 cacagactga atcagcgatt ctggtgcatg cccggcggtg tgcgccagcg gagtcgtgcg
      121 gcttcgtgat aagcaccccg gagggcgaac ggtaccagcc ctgcgtgaat atctccgcag
      181 agccggaggc gtattttcgt attgcgccgg aagactggct gcaggcacag atgcaggggg
      241 agattgtggc gctggtccac agtcatcccg gtggtctgcc ctggctgagc gaggcggacc
      301 ggcggctgca gataaagagt gccctgccct ggtggctggt ctgtcggggt gaaattcacc
      361 gcttccgctg tgtgccgcac ctgaccggac ggcgctttga acacggtgtg acggactgtt
      421 acaccctgtt ccgggatgca taccatctgg cggggattga gatgccggat tttgtgcgtg
      481 aggatgactg gtggcgcaac ggccagaacc tgtacctgga caacctggcg gaaaacggat
      541 tttaccgggt gtctccgtcc tgtgcacagg caggcgatat tctgctgtgc tgctttggtt
      601 catcggtgcc gaatcatgcc gccatttact gtggcaacgg tgacctgctt caccatatac
      661 ctgaacaact gagtaaacgg gagaggtatt ctgaaaaatg gcaacgacga acgcattctg
      721 tctggcgtca ccgccactgg tccgcatctg ccttcacggg gatttacaac gatttggtcg
      781 ccgcatcagt ctgtatgtga acacggcagc ggaggccatc cgtgccctgt cgcagcaggt
      841 gccgggattc cgctgtcaga tgaacgaagg ctggtaccag atacgtattg ccggtgagga
      901 taccgcgccg gaggcggtgt atgcccgtct tcatgaaccg ctgagcggga ggtccgtgat
      961 tcatattgta ccgaggctgg caggggccgg gggaaatggt gtttttcagg tggtgctggg
     1021 ggctgcagcc atcgtgggct ctttcttcac cgccggtgca acgatggcgt tgtggggcgc
     1081 agccctgagt gccggagggc tgactgccac cacgatgctg ttctcactgg gtgccagcat
     1141 gatactgggt ggtgtggccc agatgctggc cccgaaggca aagacgccgg agtacaaaag
     1201 cacggataac ggtaaacaga acacgtattt ttcgtcactg gacaacatga ttgcccaggg
     1261 taacccgatg ccggtgcctt acggtgaaat gctggttggt tcacgacgga tatcccagga
     1321 catcagcacc cgtgatgagg gcggtgacgg gaaagtggtg gttatcgggc ggggatgaaa
     1381 aaaaatcccg cagtgctcgg cgcctgcggg aagagaacga tgttgactaa cctgttggcg
     1441 ttttattttt atttacccgc aaaaaatgta acgcagcgtc attattgtgg ctacaggtaa
     1501 tttgcagaaa tgtgaataaa ttcagaattt ttattcacag gaaggggctg cgggatatcg
     1561 gtggcagagg acagaaaata tcaggccggg tttaagggat aaaaaaatcc cgcagagtca
     1621 gcggagctgc gggagagaac gatgaagatt aacgttatgg agttattttt caggcatcaa
     1681 aaaagtaacg cagcgtcatt attgcggcta caggcaattt gcagaaatgt gaaga
//
LOCUS       DAZUCE010000420         1728 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZUCE010000420 DAZUCE010000000
VERSION     DAZUCE010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.423"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            323..1486
                     /locus_tag="ACHY03_005141"
     CDS             323..1486
                     /locus_tag="ACHY03_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFS3908234.1"
                     /translation="MEARAEATSARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYD
                     SEDILPGIRGEMNLFASGAIEADVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALR
                     EKQSIAVLEQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLK
                     TTLGKLSTWTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKA
                     ALKAQELARYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLD
                     SVARMIDETGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNA
                     ENELYSVGLSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            1491..>1728
                     /locus_tag="ACHY03_005142"
     CDS             1491..>1728
                     /locus_tag="ACHY03_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908235.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAI"
BASE COUNT          430 a          393 c          452 g          453 t
ORIGIN      
        1 gtgaaaattt tcttcataaa ctgcaaggat tgtaagggtt tttcagcttt tcaataaaat
       61 tgaagtatct cacattttta ttatcttctg tcagcgcgga agataatggc ggtagcttgg
      121 atcaattccg gtgtctctgc atttcaattt tactcgatat tttacccggt tgcatcatga
      181 tgcctgatta tatttcccgc gtgtcccgga ggttcgggtg aagagaatat tatctttctg
      241 ttttcttttc tctgtccaca acgtgttcgc ttctccactg ccggccctcc tgagggatgc
      301 tctcattcat gatccatcag tgatggaggc aagggcagaa gccacctctg cccgttcccg
      361 tgtggagcag gctcgttcag cacactggcc ggtcgtcaca gcaacaggca gtaaactcct
      421 ttcccagagt caccgctacg actatgacta cgactctgaa gatattttgc ccggtatccg
      481 cggtgaaatg aatctgtttg cttcgggagc tattgaggcg gatgtcagcc ggagtgagtc
      541 ggaagccgag tattaccgtt ataaagttga tgaggctggc gaggaaacaa ttcgctcctt
      601 tgtttcatta tatcttgatg cgctaaggga aaagcaatcc attgcggtac ttgaacagag
      661 tctctcccgg cataacgcaa ttcttaatga tcttaacacc atcagtattc acgataccgg
      721 gcgggaatct gaatttgttc aggccgaagc ccgcaggctg atggtccggc agcagataaa
      781 tacccgcacc cgagtgctca aaaccacgct gggaaaactt tcgacctgga ccacaaatcc
      841 ggtaaccgaa gccgatcttg aaaatccttt ttcccggatg acagagtcca gattgttaac
      901 tgattttacg cagacgcctc agaaagggaa tccgtcgtgg cttgccagtc aggctgatgt
      961 agagagtaaa aaagcggcgc tgaaagcgca ggagcttgcc cggtatcccc gggtggattt
     1021 aacgggatca gtgacccggg atgacaggca gataggtgtc agtctgtcct gggacctgtt
     1081 caaccgtaat gccagttatg gtgtcagtga aaaggccgcg cagatagctg cagctaccgg
     1141 acgactggac tctgttgccc gaatgattga tgaaaccgga cgattatcgc tgataacggt
     1201 cagacaaagt cggatggaaa tggaaacgct cagacgtcag gagcaggcct ctgccagagt
     1261 tgtggacttt tatcgtcttc agttccgggt ggcaagaaaa acactgattg aattactgaa
     1321 tgcagaaaac gaactgtaca gcgtcgggct ttcccgggtt cagacagagg atcagatgct
     1381 ccacggaatg ctggattatc tgtattccca gggaatgctc ctgaaatgga gcggagtgaa
     1441 tctttctggt gcagaagaaa aagtaacagg gcagaaggac agataaataa gtgaagagta
     1501 tttttgaagg attatcacag gtaacggcat tactgggtac acctgtatct gctgagacgc
     1561 tggctgccgg tacagtccgg acagatgtct ccgggattga ttttcgttct gttggcgagt
     1621 ttcttcgcag tgaagggttt gataatcatc tttcccggcg ggctccggaa gatattcctt
     1681 cacttgcggt tcctgtgctt cttttactga atgcacagga agccattg
//
LOCUS       DAZUCE010000421         1710 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZUCE010000421 DAZUCE010000000
VERSION     DAZUCE010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.424"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            10..1707
                     /locus_tag="ACHY03_005143"
     CDS             10..1707
                     /locus_tag="ACHY03_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFS3908236.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
BASE COUNT          550 a          372 c          342 g          446 t
ORIGIN      
        1 gaataccata tggaacatca gttaaataaa aaactgctta actcatcaat atcacttata
       61 ttagcatctg gtctgatgat gagtactgca aatgcagcag atgatgttaa gctcaaagca
      121 acaaaaacca atgttgcctt tgcggacttc acgccgacgg aatacagcac taaaggtaag
      181 ccaaatatca ttgttttaac aatggatgat ttagggtatg gacaattgcc ttttgatgat
      241 agttcatttg accctaaaac gatggataac cgaaatgttg ttgatacata caaaataggt
      301 attgataaag caattgaagc tgcaaaaaaa tccactccaa cattagcggc cctgatggac
      361 gaaggtgtaa aattcactaa cggctatgtt gcacacggtg tctctggccc ttcacgtgcc
      421 gcaatcctga cggcgcgagc tcctgctcgc ttcggtgtat attcaaattc tgatgcacag
      481 gatggtatcc ctctggaaga aacattcctg tctgaattac ttcagaatca tggttattac
      541 actgcagcaa tcggtaagtg gcacttatca aaaatcagca acgtaggtgt gcctgaagaa
      601 aaacaaaccc gtgactacca cgataacttc accaccttct ccgcagaaga atggcaacct
      661 cagaatcgtg gcttcaacta cttcatgggt tatcacgcat ccggcgtttc actttataac
      721 tccccgtcat tgttccgtaa ccgtgaaaac attaaagctg aagggtacat tagtgatcaa
      781 ttaactgatg aagcaattgg tgtaattgac cgtgcaagca aactggaaca gccatttatg
      841 atttatctgg catacagtgc accacacctg cctaacgatc agcctgcgcc taaaaactat
      901 caggagaaat tcaacactgg tagcaagact gctgataact attatgcgtc aatttactcc
      961 gttgaccagg gggttaaaaa gatcctgaat acacttaagg aaaaaggtca gtacgacaac
     1021 accatcattc tgttcacctc tgataacggt gctgtaattg atggtccact gccactgaat
     1081 ggtgcgcagg ctggttacaa aggccaaccg tatccgggtg gtatgcatac acctatgttc
     1141 atgtggtgga atggcaagct gaaaacgggt gattatgaca aactggtttc atcaatggac
     1201 ttctatccga ccgctttaga tgccgcagat atcaagatcc ctgcttctct gaaactagat
     1261 ggtgtatcat tattgccata tgtacagaaa ggagctgata agaaatctgc agctggcgaa
     1321 cctcacaaat atctgtcctg gattacctct tattctcact ggtgggacga agagaacact
     1381 gcattctgga acggatatca taaattcgtg agaaaagagt ctgatgatta tccgcacaac
     1441 ccacatacag aagacttaag ccagtttgca tacactgtaa gaagcaacga ttattctctg
     1501 gtttacacct atgcagagaa taaactagct ctgtacgata tcaacgatct gcaacaaact
     1561 aaagatctct ctgcatccca tcctgatgta gttaagaata tgaaggctgt tatgattgac
     1621 ttcatcacgt caagtcaaaa accgatcagt gatatcaacc aagccaaatt tgagaatata
     1681 aaaaaatctt taactaacgt gaaatgataa
//
LOCUS       DAZUCE010000422         1706 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZUCE010000422 DAZUCE010000000
VERSION     DAZUCE010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.425"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(266..430)
                     /locus_tag="ACHY03_005144"
     CDS             complement(266..430)
                     /locus_tag="ACHY03_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFS3908237.1"
                     /translation="MTVKLRLAAVALLLFLVVMVDFTSRIMSVLADGVLVVGIVVVLF
                     PLVKKDMPGS"
     gene            complement(427..858)
                     /locus_tag="ACHY03_005145"
     CDS             complement(427..858)
                     /locus_tag="ACHY03_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFS3908238.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATITGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(1028..1104)
                     /locus_tag="ACHY03_005146"
     tRNA            complement(1028..1104)
                     /locus_tag="ACHY03_005146"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1068..1070),aa:Arg,seq:tct)
     gene            complement(1118..1194)
                     /locus_tag="ACHY03_005147"
     tRNA            complement(1118..1194)
                     /locus_tag="ACHY03_005147"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1158..1160),aa:Arg,seq:tcg)
     gene            complement(1204..1279)
                     /locus_tag="ACHY03_005148"
     tRNA            complement(1204..1279)
                     /locus_tag="ACHY03_005148"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1244..1246),aa:Ile,seq:cat)
     gene            complement(1320..>1706)
                     /locus_tag="ACHY03_005149"
     CDS             complement(1320..>1706)
                     /locus_tag="ACHY03_005149"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS3908239.1"
                     /translation="GELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYT
                     DVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRR
                     ATGVELETERFEQTVREVQDLASQNG"
BASE COUNT          407 a          520 c          385 g          394 t
ORIGIN      
        1 cccctccgga gaggggctaa agccgcgtat ctgtatcatc atgcgcatga tgccgggtgc
       61 ctcccggtga gttcagcccg gtgccaccaa acccgcgcgt ttactttcct aggtatcaaa
      121 gataacacct aatgcaccag tcgcccctcc gctcaggggg attcaccatg cctgtttctt
      181 ttaacaaact ccccgcaaaa cagacaactg tcaaccgtct gaattgtgag acatttaaaa
      241 ttttcggggc atgactgata cccggctaac tacctggcat gtcctttttc accaaaggaa
      301 aaagcaccac cacaataccg accaccagca ctccatccgc cagcaccgac atgatcctgc
      361 tggtgaaatc caccatcacc accagaaaca gcaggagtgc cacagcggcc agacgcagtt
      421 ttaccgtcac aggtggttct ccagtcgcag gccaagaaca ccagcaatct cttccagaac
      481 cttacgctct tccggctcaa tttcaccatc tgcttcagca atggccaccg ccacatccag
      541 cacatcttcc gcttcacgcg tatcgtgttt cacatcttca atttcacgca acgccgctcg
      601 acgaccaatt ttaaagttgg tgtcaagctg acctgtgatc gtggcactga tagcattaat
      661 ttctgacgta aacgcggaca acgctggctg gttacgcaag acctgctcga tcttcgcttt
      721 ctctgaagcc tcacattcac catctgcata ggctaccagg taggcagcat taataaccgc
      781 ctgtgccaga tcacgtttct caaacttttt aatttccgct gctgctctgc gggttttctt
      841 tttgaaaata ccaaacatcg tgacgttcct ttaggtgggt gagccaacgc ccgggagcga
      901 tctgcccaca gagaaagtca cactgaccac tccgtaagct cacccccgaa aggctctgtg
      961 gttgatatgc gccgggcgtg gcgcggatac aaaaaaggcc cgcaaaagcg agcctggtaa
     1021 ataaatatgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga aggcaattgc
     1081 tctgtccggc tgagctaaca acgctgaata ccgataatgg accgccatcg aggactcgaa
     1141 ccccgcgcaa ccagcttcga aggctgacgc tctatcccga tgagctaatg gcggtatgtg
     1201 atatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc gctcgctctc
     1261 accactgagc taaagggccg agtgcaggat aataacggta cgtaactaat cctgcaatat
     1321 catccgttct gactggctaa atcctgaact tccctgaccg tctgctcaaa acgttcagtc
     1381 tccagctcaa caccagttgc acgacgcccc agtgccatcg cggctttgac tgtcgaaccc
     1441 gaccccatga aaaaatctgc aaccaggtcc cccggacgac tgctcgcgct gattatctgc
     1501 tgcagcattt ctgccggttt ttcgcacgga tgtttcccgg gatagtactg caccggttta
     1561 tgcgtccaca catccgtgta cggcacctgc gccgtcacgc caaaataccg ccgcagatgc
     1621 ttatattcac tctgcagttc cgcatactgc cggttcagtg aagtatacgt ctccagcagc
     1681 tggtggtggg gcttctccag ttcacc
//
LOCUS       DAZUCE010000423         1690 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZUCE010000423 DAZUCE010000000
VERSION     DAZUCE010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.426"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(248..1120)
                     /locus_tag="ACHY03_005150"
     CDS             complement(248..1120)
                     /locus_tag="ACHY03_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFS3908240.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT          415 a          400 c          446 g          429 t
ORIGIN      
        1 gaggtccggc tgacggaagt ttctgaattg tggttctgtt gttccctgaa tctcatgtct
       61 gtgtgtatct gcgcacgttg ctaacaggag ttattattag cataacgatc gatattaacg
      121 atcggtgaaa taggtatcga tcgttttatt ctattgtcaa gctattgcac ggacaagaga
      181 gacagtaacg aaaacagcgc ttttgtgagc agacccagag gcagggagga catcatccac
      241 gacaggttca gaagaaaatc cagttccata ccgcacgggc aacagagacc accgtgtcac
      301 gaacagcgcg cagggccgta cggacaacag aggcaacaca gacgctctcc gctgtgtcaa
      361 atatccggtc caccgcaccg gtaaactgtt cacgggcctg agagcggaca gattccgtgc
      421 gcagcccgtc ctgcagtcgg gtcatcaggg gactggcggc atggtcggga agcgctgtca
      481 tgagcgcact gaccagcgta tccagttccc agccggtgcg ggccgatacg gccacaaccg
      541 gatgtacggg ccggaacaga cggaataccg cctccgtttt ctcccgaatg ttctgtgcct
      601 gtgcgggaga aggctgaata ccggccatat cccattcatg gcagggctcc gttttatcgg
      661 cctgcgtcac cacaaacagt acccgctgat gtccccggtg cagtatgtgt cgccagaaat
      721 actcatccac agacagggca cggtcatcgg ctttaatcag ccacagtacc aggtccagtt
      781 ctggcagaat gtcacggtac agggcctcat actctgcatc cctgtcccgg ctctcgccca
      841 ccccgggcag gtcagtgata accatgctgt gaccgtgacc actcagacgg aagcgctgca
      901 cttcccgggt gccggcgtga acatcactga ccggtgtgac ctccccctga aacagtgcat
      961 tacagagtga agatttaccg gccccgcttt tacccataat gccaatcacg ggttcgtgac
     1021 tggtgagttt gcgcagatgt tccaggatgt gacgtgaaag cgagtaaggc agggaagaaa
     1081 ggggtttttc aattgcctca atggcatcag acggattcat acatgtattc ccggtgaaaa
     1141 aagacaaaac cccgcgcacc ggaaaggtgg cgggggatcc acggagataa tatctgtctg
     1201 aaattgtttg gaatcgatgg ggatgttgcg tattaacatg aaggagggca ggagttatcc
     1261 gttactgggt gatatccttc catgaaaata cgcggttatt gttaaacagc aggctgatta
     1321 ctccgtaaag gagaaaaata ttcatcttgc agcctgatta tcaggtgtgt ttatgtttac
     1381 tgaggtgagt atttttcagt atattgtcgg ggcagtcagt gggagatatt aaatttacgt
     1441 ttagagttat cttgcttctg ctgtgtattt ttataagagt gcgcacgtaa tgatatctgt
     1501 tgataccatg tggttaagtg tttcccttcc tggtcatgcg taatgattaa atgcttttgt
     1561 gacaccattc ttccggcatg aatatttatc cagttccctt ttttatttgt gtagtgatac
     1621 tgtcggaata gcgttcagta atgtgaagag tcctgttaaa aatctttaat aaatgtgtca
     1681 ccctggtcat
//
LOCUS       DAZUCE010000424         1678 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZUCE010000424 DAZUCE010000000
VERSION     DAZUCE010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.427"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(40..372)
                     /locus_tag="ACHY03_005151"
     CDS             complement(40..372)
                     /locus_tag="ACHY03_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFS3908241.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNKDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(575..880)
                     /gene="ydfV"
                     /locus_tag="ACHY03_005152"
     CDS             complement(575..880)
                     /gene="ydfV"
                     /locus_tag="ACHY03_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFS3908242.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLAFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            905..1144
                     /gene="relB"
                     /locus_tag="ACHY03_005153"
     CDS             905..1144
                     /gene="relB"
                     /locus_tag="ACHY03_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFS3908243.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            1144..1431
                     /gene="relE"
                     /locus_tag="ACHY03_005154"
     CDS             1144..1431
                     /gene="relE"
                     /locus_tag="ACHY03_005154"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFS3908244.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            1503..1658
                     /gene="hokD"
                     /locus_tag="ACHY03_005155"
     CDS             1503..1658
                     /gene="hokD"
                     /locus_tag="ACHY03_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFS3908245.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          428 a          365 c          412 g          473 t
ORIGIN      
        1 cgaaaagtgg cccgatgagg gccacaatac ggctgtcact tagacgtaaa tatcaatggt
       61 gccagcggta tttgtatcgt cttttttctc ttctttttta tcaggctgaa ctgtcgcgtc
      121 tttattcttt ttctctgcct gctgccttaa caactgctcc agttgagccc tgaggctttc
      181 aatttgcttc tgtaccaatg cagccatttc ttttttctgc tgtgtcgtca tcccctcttc
      241 cgatgagatt ttcccaagct tttcagtcaa cacctgaatt tgtcttgtga ttttggctat
      301 ttctgatgtt ccttccgggg cggagttgtt tgaaataacg gttgaggtat ttccctgaat
      361 tgtgacagac atagatttct ccttttaaaa aagcactatc ggcatgcaca aaaaaatctt
      421 taatcgtatt tcttgtgtca ttaattgttt gatgttcaga ttgttttcct cgcgggctgg
      481 cgcgcctcag aaagtaaagc ttgttgacag gggtaaacgt tcggcaataa ttttctgccg
      541 catgcgggtg ttgcataaaa cgtgctacgt tcctttatcg acaggtcagg tcaccgctca
      601 cccgccgacg agaaagcaac actgacatgc taaagcaaaa aatagatgaa taagttgagt
      661 tgtgcatatg tagcctgacc gtcacaaagt atatggtgtc tgtaccagta agatgatggc
      721 cggactcttt aaaagcgagc tgacctgcac aatacaggat ggacttagca atggctgctc
      781 ctggcacaaa gcggacagtg atcaccgttc ttacgactac tttctgactt ccttcgtgac
      841 ttgccctaag catgttgtag tgcgatactt gtaatgacat ttgtaattac aagaggtgta
      901 agacatgggt agcattaacc tgcgtattga cgatgaactt aaagcgcgtt cttacgccgc
      961 gcttgaaaaa atgggtgtaa ctccttctga agcgcttcgt ctcatgctcg agtatatcgc
     1021 tgacaatgaa cgcttgccgt tcaaacagac actcctgagt gatgaagatg ctgaacttgt
     1081 ggagatagtg aaagaacggc ttcgtaatcc taagccagta cgtgtgacgc tggatgaact
     1141 ctgatggcgt attttctgga ttttgacgag cgggcactaa aggaatggcg aaagctgggc
     1201 tcgacggtac gtgaacagtt gaaaaagaag ctggttgaag tacttgagtc accccggatt
     1261 gaagcaaaca agctccgtgg tatgcctgat tgttacaaga ttaagctccg gtcttcaggc
     1321 tatcgccttg tataccaggt tatagacgag aaagttgtcg ttttcgtgat ttctgttggg
     1381 aaaagagaac gctcggaagt atatagcgag gcggtcaaac gcattctctg aaccaaagca
     1441 tgacatctct gtttcgcacc gaaggtgaca cttctgcttt gcgttgacag gagaagcagg
     1501 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag
     1561 tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg
     1621 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gacccggcgg gggagaaa
//
LOCUS       DAZUCE010000425         1676 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZUCE010000425 DAZUCE010000000
VERSION     DAZUCE010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.428"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1452
                     /locus_tag="ACHY03_005156"
     CDS             <1..1452
                     /locus_tag="ACHY03_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFS3908246.1"
                     /translation="LASGATWNIPDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLK
                     VKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKG
                     IQVVEAINGATTEEGAFVQGNMLQAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASM
                     LTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYG
                     FVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLV
                     HTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQ
                     YTWQGLSLDDGQDNTGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAKH
                     SVRELPVNWWVQPSVIHTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVR
                     ENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            1573..>1676
                     /locus_tag="ACHY03_005157"
     CDS             1573..>1676
                     /locus_tag="ACHY03_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908247.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGL"
BASE COUNT          376 a          474 c          491 g          335 t
ORIGIN      
        1 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat
       61 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
      121 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
      181 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggtggcag ggcaaccgga
      241 aaaaccatcc tgaatctggt gaacgccggc aacagtggta cggggctggc gaccaccggt
      301 aagggtattc aggtggttga ggccattaac ggtgccacca cggaggaagg ggcttttgtc
      361 caggggaata tgctgcaggc cggggccttt aactacaccc tcaaccggga cagtgatgag
      421 agctggtatc tgcgcagtga agaacgttat cgtgctgaag tccccctgta cgcctccatg
      481 ctgacacagg caatggacta tgaccggatt ctggcaggct cccgcagcca tcagaccggt
      541 gtaaacggtg aaaataacag cgtccgtctc agcattcagg gcggccatct cggtcacgat
      601 aacaacggcg gtattgcccg tggggccacg ccggaaagca gcggcagcta tggcttcgtc
      661 cgtctggagg gtgacctgct cagaacagag gttgccggta tgtctctgac gacaggggtg
      721 tatggtgctg caggccattc ttccgttgat gttaaggatg atgacggctc ccgcgccggc
      781 acggttcggg atgatgccgg cagtctgggc ggatacctga atctggtaca cacatcctcc
      841 ggcctgtggg ctgacattgt ggcacaagga acccgccaca gcatgaaagc atcatcggac
      901 aataacgact tccgcgcccg gggctggggc tggctgggct cactggaaac cggtctgccc
      961 ttcagtatca ctgacaatct gatgctggaa ccacaactgc agtacacctg gcagggactc
     1021 tccctggatg acggccagga taacaccggt tatgtgaagt tcgggcatgg cagtgcacaa
     1081 catgtgcgtg ccggtttccg tctgggcagc cacaacgata tgaactttgg taaaggcacc
     1141 tcatcccgtg acaccctgcg cgacagtgcc aaacacagtg tgcgtgaatt gccggtgaac
     1201 tggtgggtac agccttctgt tatccacacc ttcagctccc ggggagacat gagcatgggt
     1261 acagccgcag ccggcagtaa catgacgttc tcaccgtccc ggaatggcac gtcactggac
     1321 ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca ccctgggcgt tcaggccggt
     1381 tatgcccaca gtgtcagcgg cagcagcgcc gaaggctata acggtcaggc cacgctgaat
     1441 gtgaccttct gacagcacca tcgcctctct gtggtcccgg tcatcatgat cgggatccgg
     1501 accggcgcaa cggatattca acgccacatt cgctggcatt aacaataaca tgatattcat
     1561 cacggagtga ctatgttaca gatagtcggc gcgctgatcc tgctgatagc aggatttgcc
     1621 attcttcgcc ttttgttcag agcattaatc agcacggctt ctgcgctggc agggct
//
LOCUS       DAZUCE010000426         1676 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZUCE010000426 DAZUCE010000000
VERSION     DAZUCE010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.429"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1452
                     /locus_tag="ACHY03_005158"
     CDS             <1..1452
                     /locus_tag="ACHY03_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFS3908248.1"
                     /translation="LTSGATWNIPDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLK
                     VKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSASGLATSGKG
                     IQVVEAINGATTEEGAFVQGNKLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASM
                     LTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYG
                     FVRLEGDLLRTEVAGMSVTAGIYGAAGHSSVDVKDDDASRAGTVRDDAGSLGGYLNLT
                     HTSSGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQ
                     YTWQGLSLDDGKDNAGYVKFGYGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKH
                     GVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVR
                     ENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            1573..>1676
                     /locus_tag="ACHY03_005159"
     CDS             1573..>1676
                     /locus_tag="ACHY03_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908249.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGL"
BASE COUNT          375 a          471 c          494 g          336 t
ORIGIN      
        1 ctgacatccg gtgccacctg gaatatcccc gataacgcca cggttcagtc ggtggtggat
       61 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
      121 gcaaccctga aagtgaaaaa cctgaacggg cagaatggca ccatcagcct gcgtgtacgc
      181 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggtggcag ggcaaccgga
      241 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
      301 aagggtattc aggtggtgga agccattaac ggtgccacca cggaggaagg ggcctttgtc
      361 caggggaata agctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
      421 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
      481 ctgacacagg caatggacta tgaccggatt ctggcaggct cacgcagcca tcagaccggt
      541 gtaaacggtg aaaataacag cgtccgtctc agcattcagg gcggccatct cggtcacgat
      601 aacaacggcg gtattgcccg tggggccacg ccggaaagca gcggcagcta tggcttcgtc
      661 cgcctggagg gtgacctgct gagaacagag gttgccggta tgtctgtgac cgcagggata
      721 tatggtgctg caggccattc ttccgttgat gttaaggatg atgacgcctc ccgtgccggc
      781 acggtccggg atgatgccgg cagccttggc ggatacctga atctgacaca tacatcctcc
      841 ggcctgtggg ctgacattgt ggccctggga acccgccaca gcatgaaagc gtcaacggac
      901 aataacgact tccgcgcccg ggggtggggc tggctgggtt cactggaaac cggtctgccc
      961 ttcagtatca ctgacaatct gatgctggag ccgcaactgc agtacacctg gcagggactc
     1021 tccctggatg acggtaagga taacgccggt tatgtgaagt tcgggtatgg cagtgcacaa
     1081 catgtgcgtg ccggtttccg tctgggcagc cacaacgata tgacctttgg tgaaggcacc
     1141 tcatcccgtg acaccctgcg cgacagtaca aaacacggtg tgagtgaact gcctgtgaac
     1201 tggtgggtac aaccttctgt tatccgcacc ttcagctccc ggggggacat gagcatgggt
     1261 acagctgcag ccggcagtaa catgacgttc tcaccgtccc ggaatggcac gtcactggac
     1321 ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca ccctgggcgt tcaggccggt
     1381 tatgcccaca gcgtcagcgg cagcagcgct gaagggtata acggtcaggc cacactgaat
     1441 gtgaccttct gacagaacca tcgcctctct gtggtcccgg tcatcatgac cgggacccgg
     1501 accggcgcaa cggatcttca acgccacatt cgctggcatt aacaataaca tgatattcat
     1561 cacggagtga ctatgttaca gatagtcggc gcgctgatcc tgctgatcgc aggatttgcc
     1621 attcttcgcc ttttgttcag agcattaatc agcacggctt ctgcgctggc agggct
//
LOCUS       DAZUCE010000427         1654 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZUCE010000427 DAZUCE010000000
VERSION     DAZUCE010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.430"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..301)
                     /locus_tag="ACHY03_005160"
     CDS             complement(<1..301)
                     /locus_tag="ACHY03_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFS3908250.1"
                     /translation="MNRKYYFNNMWWGWFMGGYTLYMSWDYEFKYRLLFWCISLCGMV
                     LYPVARWCIENTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLTLIYI"
     gene            430..777
                     /locus_tag="ACHY03_005161"
     CDS             430..777
                     /locus_tag="ACHY03_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFS3908251.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLLPLPF
                     MPFWQVTVLMGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(797..1387)
                     /locus_tag="ACHY03_005162"
     CDS             complement(797..1387)
                     /locus_tag="ACHY03_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908252.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQINESAAP"
     gene            complement(1387..1644)
                     /locus_tag="ACHY03_005163"
     CDS             complement(1387..1644)
                     /locus_tag="ACHY03_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908253.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPENDR"
BASE COUNT          371 a          460 c          420 g          403 t
ORIGIN      
        1 gaatatatat cagagttaac ggaagagata atatgaaaac agtacccgta taaaccgcaa
       61 gtaatcccat ttttccggga gtatcagcaa aaaaaccgct gttccagaaa tcaggccggg
      121 taaatttcag ggcagtattt tcaatacacc atctggcaac cggatacaaa accattccgc
      181 agagagaaat acaccagaac agcaatctgt atttaaactc ataatcccat gacatataca
      241 gagtataccc acccataaac catccccacc acatattatt aaaataatat tttctgttca
      301 tctgcctgtt ttcctttctt ttgcactttt gtcaatgtac tgatgcatga caacataacc
      361 acagcatgat aataataatc aataacaata agttgtgtca cgtttacatc atggaacgga
      421 ggtgctcctg tgacctttga gcagaaaaaa gcccgggcca ttgccctgat ggacagcaaa
      481 aagatgtggc ggagcaatta tgcccctcct cttctgcgta ttctctggcg gctgggtatc
      541 cggcttctgc cattaccctt tatgccgttc tggcaggtca cggtgctgat ggggggctta
      601 tggggcattt cctggggatg tgcaatgtgg tttatctact gggggccgtc aggaatggtg
      661 gcaggtgagg ccattattat aagtatcacg gggggttttt tgttcggact gtgcatggcc
      721 tctttccact ggtggcgcag gaaagttaac cggctgccac catgggatga tgtgtaaacc
      781 gtgcacaggc gtgttcttac ggagcggcac tttcatttat ctgtacgcga acttctattc
      841 ctgcgtcatg acaggcctgc agccactgcg ccacttccag cggatcgccc tcccggcgta
      901 ccaccctgcc ttctttattc cataactgca gacaggtgct gccgtcgaga cgcaccacaa
      961 aatccccacg gcaggcctga tacgttatgc cgttcagctc caccgccaga tcagtggcag
     1021 aagtgccgtc tgcgggcaga cctgctgccg ggatcagcgg cagtacgacg acttctgccg
     1081 cacgctgttc tgcccggagg cggtcctgtt ctgccagcag cagaggctgt gccacggccc
     1141 ggccggcgtc cgtcagctcc actgccagct gcaggttcgg agcgcgcagg gtacgcagcc
     1201 agccggcatc ctccagacga cggcaggagg cgcgcaggtt tggcccgtag accggggctt
     1261 ccccgcttcg ctccagcacc cgttctatgt cccgcgttag cacggggcct ggccgttttt
     1321 catcaagggc agccagcaca atcagcactc tccgctgcag tggcgacggt cggcggcttt
     1381 gggtcattac ctgtcattct ccggccagat gaggtttctg aggcgctgaa agtcggcgtc
     1441 aatgagctcc tgtctcttta tggcattcat tgaggcatag ccatttatgg ccagttcatg
     1501 ccagaagatc agcacggctc cggctttggc tgactcgcag agataaatcg cgccctggct
     1561 gtccagattg cattcccgga tgcgtttttc cgcaaaccgg gtcagttgtt catagctgag
     1621 tgaaaacacc ctttcttcat ccatcctgcg cgct
//
LOCUS       DAZUCE010000428         1648 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZUCE010000428 DAZUCE010000000
VERSION     DAZUCE010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.431"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            388..>1648
                     /locus_tag="ACHY03_005164"
     CDS             388..>1648
                     /locus_tag="ACHY03_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908254.1"
                     /translation="MVTRTMQQKYQYDRVLSGELHPFPGIYEAVSEQILLLIFKARQL
                     YQDPEYRPWFEEQHRVLSALRANTELLRIFREQRSAEEPKLTIEESPSPQPTPSALTD
                     ALNVMCHLYNGFDEGNISLKTWGAMQEVQTTSTFISPGPRTWKTPELAKFVGDDLFNI
                     LTECKAWSSPIPAETRQGYCHQDLSVAHSCTSELLAHLNQYHEIDESAILARVIGITV
                     NGFYKKNNTDLPEWLSPFKEEHKIFRDCPEQNTGDVIRRKEQLLKTGRTEFVNILCRV
                     ANWYEIESIYDEYVRLYANEIIDWFNKTMKYFKPTDFIRYTWKFATVTPDKDTETGHT
                     TAWEKDYCQITIQRDELLRLIHTNTLQDAVIYLCHCLQEKYKKSSQNETEFPGIKPPA
                     SESEINITDNFEFHSLLELSKDNEKRLE"
BASE COUNT          576 a          333 c          333 g          406 t
ORIGIN      
        1 ataaattcat gtaaaattca aacaaggcat ggaggagtcg caggaaggtg aaatatttcc
       61 atctaatttg tgataatcat cacaaaaata agctacaaca agcggaggaa aagacaatct
      121 gttgctgaac agacagaagc aggctacgaa tgatcggaaa atgaagcaaa tacgcttata
      181 cagaagactc agaggtaact gacggagaca atcacaatca ggagaaaatt atcgtaaata
      241 atgtataaaa tctcaggata taaacaaatg tacacaagct atccacgtaa tcagcggctg
      301 gctcaggatt aacgtttcat ttcagtggac aacatcgatt ctgtctgaca acggaccaat
      361 atgtagcaag cagaaacttc tgacatcatg gtaactcgga caatgcaaca aaagtatcag
      421 tatgatcgtg ttttatccgg cgaactccac ccattccctg gcatatacga ggcggtttcc
      481 gaacaaatcc ttttgctcat ttttaaagca cgtcaactat atcaggatcc agaatatcgc
      541 ccctggtttg aagaacagca cagagtactc tctgcattac gtgccaatac agagctcctg
      601 agaatcttta gagaacaaag aagcgctgaa gaaccaaagc ttactattga agagtctccc
      661 tccccccaac ccactccttc tgctcttaca gatgcgctta atgtgatgtg ccatttatac
      721 aatggttttg atgaaggaaa tatcagtctg aaaacctggg gggcgatgca agaggtacag
      781 acaacatcaa cctttatcag cccaggaccg agaacctgga aaacacctga actggcaaag
      841 tttgtaggag atgatttatt taatattctg actgaatgta aggcctggag ttcaccaatt
      901 ccagcagaaa cccgacaggg gtattgccat caggatctgt ctgttgcaca ctcctgcacc
      961 tcagagttac tggcacacct gaatcaatat catgaaattg atgaatcagc aatacttgcc
     1021 agagttattg gtataacagt taacggattc tacaagaaaa ataatacaga ccttccagaa
     1081 tggctatccc cctttaaaga ggaacataaa atattcagag attgtccgga gcaaaatacc
     1141 ggggatgtaa tcagacggaa agaacaactg ttgaaaacag gacgtacaga attcgtgaat
     1201 attttgtgtc gggtcgccaa ctggtatgaa attgagtcaa tatacgatga atacgtccgt
     1261 ctttatgcca atgaaatcat tgactggttc aacaaaacca tgaagtattt taaaccaaca
     1321 gattttatcc gatatacatg gaaattcgca acagttactc cggataaaga tacagaaacc
     1381 gggcatacaa ctgcatggga aaaggattat tgccagataa ccatacaacg tgatgaactt
     1441 ctgaggttga ttcacactaa tacccttcag gatgctgtaa tttatctttg tcattgtctg
     1501 caggagaaat acaagaaatc atcccagaat gagacagagt ttccaggaat aaaacctccg
     1561 gcatcagaga gtgaaattaa tataacagat aattttgaat ttcattctct tctggagcta
     1621 agtaaagata atgagaagcg gctggaaa
//
LOCUS       DAZUCE010000429         1639 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZUCE010000429 DAZUCE010000000
VERSION     DAZUCE010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.432"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>1639
                     /locus_tag="ACHY03_005165"
     CDS             <1..>1639
                     /locus_tag="ACHY03_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFS3908255.1"
                     /translation="LAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTAFF
                     EAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTLDTLDVE
                     AAAATLPMDFNIYEIPVSVYRRAKEIVLKKESPFREWSAALRATPGILDYSRAAIFAL
                     IRSAHPEFYHYPGRLQGYINANLTETDHENPTAETLMAARHTPEKDILEEINGGLAAE
                     CKTEEEKNDEENPQPSGAMADEQATAETMAADTVEHHQDPQPLDDKSQVKVTAEEVNK
                     IMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETCDSVNQNQQETEQNDQK
                     AEQNSQNALQDEPETKQSEPVAQQEEEKVCTACGQSGGGNCPDCGAVMGDATYQETFD
                     EKNQAEVQEDDPEEIEGAEHPNNENAGNDQHHTSDSETGEAADPLIAVNGHHEITSTS
                     RLWHHLMIDLETMGKNPDAPINSLAGKFFDPATGEMGPEFSKTIDLETAGGVIDRDTI
                     KWWLKQSREAQSAILTDEIPLDDALLQLREFINENSG"
BASE COUNT          508 a          400 c          414 g          317 t
ORIGIN      
        1 ccctggctat ttcccacaac acaaagctgc tcgacccgct ggagattcgc gaccttcaca
       61 aactggtacg cgacacagac aaagttttcc ctaatcccgt taattccagt ctggggttaa
      121 tgactgcttt tttcgaagca tacctggacg ctgactatac cgatcgaggt ctgctgacaa
      181 aagagtggat gaaaggaaat cgtgtttctc gcatcacccg cacggcttcc ggtgcaaatg
      241 ccggtggcgg aaataaaacc gatcgtaatc ctgacttagt acacaccctc gatactctgg
      301 atgtggaggc tgcagcagcc acacttccga tggattttaa tatttatgaa atacccgtga
      361 gcgtttatcg tcgcgcaaaa gaaatcgtcc tgaaaaaaga aagtccgttc agggaatggt
      421 ccgcagcact tcgcgcaacc cctggtatcc tggactattc ccgcgcagct atttttgcac
      481 ttatccggag cgcccaccct gagttttatc actacccggg acgccttcag gggtatatca
      541 atgccaacct gacagaaact gatcacgaga atcccacagc tgaaacgctc atggctgccc
      601 ggcatacacc ggaaaaagat atcctggaag aaattaacgg cggactggct gctgagtgca
      661 aaacagaaga agaaaaaaat gatgaagaaa acccgcaacc atctggcgca atggcagatg
      721 aacaggcaac ggctgaaaca atggcagcgg atacagttga acatcatcag gacccgcagc
      781 cgctggatga caagtcacag gtaaaagtta ccgctgaaga agtaaataaa attatggagg
      841 cagcagacat cagtcgacca gatgccgaca aattgcttgc cgcttctcgc ggtgagtttg
      901 ttgaagggat tagcgacccg aatgattcga aatgggttaa ggggattgaa acctgcgatt
      961 ctgtgaacca gaaccagcaa gaaacggaac agaacgacca gaaagcagaa caaaacagcc
     1021 aaaatgcgtt acaagacgag ccagaaacga aacaatccga accagtagcg caacaggaag
     1081 aggaaaaagt ctgcaccgcc tgcggtcaga gcggtggcgg caactgtcct gactgtggcg
     1141 cggtgatggg cgacgcaaca taccaggaaa cattcgatga aaaaaatcag gctgaagttc
     1201 aggaagatga tccggaggaa atagaaggcg ctgaacatcc gaacaatgag aatgctggta
     1261 acgatcagca tcacaccagc gatagtgaaa ctggcgaagc ggcagatccc ttaattgcgg
     1321 tgaacggtca tcacgaaatc acatccacca gcaggttgtg gcaccatctg atgattgacc
     1381 ttgaaacaat gggcaaaaat cctgatgcgc caataaactc tctggccggt aagttttttg
     1441 atccggcaac cggagagatg gggccagaat tcagcaaaac tatcgatctg gaaaccgcag
     1501 gcggagtcat cgatcgggac accattaagt ggtggctgaa acagtcacgg gaagcacaat
     1561 cagccattct gaccgatgaa atcccgttgg atgacgcact gttgcaatta cgggaattta
     1621 tcaacgaaaa ctccggtga
//
LOCUS       DAZUCE010000430         1630 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZUCE010000430 DAZUCE010000000
VERSION     DAZUCE010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.433"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..186
                     /locus_tag="ACHY03_005166"
     CDS             <1..186
                     /locus_tag="ACHY03_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908256.1"
                     /translation="TKAFNHYVRIPLYSITVWPNRDRFRCHFASTHDPEGKHLTTWYQ
                     QISLRVHSYKKHTEEVR"
     gene            513..1385
                     /locus_tag="ACHY03_005167"
     CDS             513..1385
                     /locus_tag="ACHY03_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFS3908257.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILPRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT          413 a          437 c          383 g          397 t
ORIGIN      
        1 acaaaagcat ttaatcatta cgtgcgtatt cctctctatt cgatcacggt ttggccaaac
       61 cgtgatcgat tcagatgcca ttttgcaagt acgcatgacc cggaagggaa acacttaaca
      121 acatggtatc aacagatatc attacgtgtg cactcttata aaaaacacac agaagaagta
      181 agataattat aaacgtaaat ttaatatctc ccactgacat ctcccccaca atacactgaa
      241 aaatactcac agcagtaaac acagacacac ctgataatca tgcagccgat aaatattttc
      301 ccctttacgg agaaatcagc ctgctgttta acaataaggg cgtattttca tggaaggaga
      361 tcacccagta acggataagt cctgatctcc ttcatgttaa tacgcaacaa tcccatcgat
      421 tcaaaacaat ttcagacaga tattatctcc gtgaatcccc cgccaccttt ccggtgcgcg
      481 gggttttgtc ttttttcacc gggaatacat gtatgaatcc gtctgatgcc attgaggcaa
      541 ttgaaaaacc gctctcctcc ctgccttact cactttcccg tcacatcctg gaacatctgc
      601 gcaaactcac cagccacgaa cccgtgattg gcattatggg aaaaagcggg gccggtaaat
      661 cttcactctg taatgcactg tttcaggggg aggtcacccc ggtcagtgat gttcacgccg
      721 gcacccggga agtgcggcgc ttccgtctga gtggccacgg tcacagcatg gttatcactg
      781 acctgcccgg ggtgggcgag agccgggaca gggatgcaga gtatgaagcc ctgtaccgtg
      841 acattctgcc agaactggac ctggtactgt ggctgattaa agccgatgac cgtgccctgt
      901 ctgtggatga gtatttctgg cgacacatac tgccccgggg acatcagcgg gtactgtttg
      961 tggtgacgca ggccgataaa acggagccct gccatgaatg ggatatggcc ggtattcagc
     1021 cttctcctgc acaggcacag aatattcgcg aaaaaacgga ggcggtattc cgtctgttcc
     1081 ggcccgtaca tccggttgtg gccgtatcgg cccgcaccgg ctgggaactg gatacgctgg
     1141 tcagtgcgct catgacagcg cttcccgacc atgccgccag tcccctgatg acccgactgc
     1201 aggacgagct gcgcacggaa tctgtccgct ctcaggcccg tgaacagttt accggtgcgg
     1261 tggaccggat atttgacaca gcggagagcg tctgtgttgc ctctgttgtc cgtacggccc
     1321 tgcgcgctgt tcgtgacacg gtggtctctg ttgcccgtgc ggtatggaac tggattttct
     1381 tctgaacctg tcgtgactga tgccctccct gactctgagt ctgctcacaa aagcactgtt
     1441 ttcgttactg tctctcttgt ccgtgcaata gctcaataat agaataaaac gatcgatatc
     1501 tattttatcg atcgtttata tcgatcgata gactaataat aacctttgtc agtaacatgc
     1561 acagatacgt acagaaagac attcagggaa caacagaacc acacgtcaga aactcccaca
     1621 gccggacctc
//
LOCUS       DAZUCE010000431         1625 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZUCE010000431 DAZUCE010000000
VERSION     DAZUCE010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.434"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(196..>1625)
                     /locus_tag="ACHY03_005168"
     CDS             complement(196..>1625)
                     /locus_tag="ACHY03_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS3908258.1"
                     /translation="PATTTFRINAPAAPASIELTPGYYQITAVPVLAVYDPTVQSEFW
                     FSEKRITDMAQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASG
                     RASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQTVS
                     KTLEDQSATIQQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLS
                     NILLQADRISMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGEPPTFSLTP
                     DGKLTARNADISGNINANSGTLNNVVIAENCTIKGTLRAENIIGDVVKTHNVSLPALS
                     AAGDHRHATERTVTVIDDQSFDRNIVIPPVFYSGIVYQGSDGDGATSICTLTVSVNDR
                     VVFSKSSSIGTGRTSGGYPVQKDTMYPGSSTPFSYFMRMASGGGDVRIKFRIEVIYYG
                     TAPGFMTTGIQITTMKATASGFMES"
BASE COUNT          341 a          476 c          382 g          426 t
ORIGIN      
        1 cgcttgctcc ttttacggag aggcgctaac cttcaatttg ctgaagatcg aaagtcaggc
       61 ctcgggttaa acatgaatgt ttgtccgggg ccttcgtctt tcaggccttc aggtgttccc
      121 tccggccatc agccaaaggc acccgcgcat actgtacggt ttttgtctcc ttccggcaat
      181 cccgggcgtc ccggattaac tttccataaa tcctgaggct gtcgccttca tcgttgtgat
      241 ctgtatccct gtggtcataa aacccggtgc tgttccataa tagatgacct caatccggaa
      301 cttgatcctg acatctcctc cccctgacgc catcctcatg aaatagctga acggagtgga
      361 tgacccggga tacatggtgt ctttctgaac ggggtatcca cctgacgtac gcccggtccc
      421 gatggatgag cttttgctga acactacccg atcattcaca cttaccgtca gcgtacagat
      481 tgaagttgca ccatcaccat cacttccctg gtagacaata ccggaataaa aaaccggcgg
      541 gataacaatg ttccggtcaa acgactgatc atcaatgaca gtaaccgttc gttctgtcgc
      601 gtgtctgtga tcccctgctg cactgagagc aggaagactg acattgtgcg ttttcaccac
      661 atcgccaatg atattttccg ctctcagagt acctttgatg gtacagttct ccgctatcac
      721 gacattattg agcgtgcccg agttcgcatt aatattcccg ctgatatccg cattacgggc
      781 tgtcagtttc ccgtccggcg tcagggaaaa cgtcggaggc tcacctgatg aggtaatact
      841 caccgcaaac agtcgtttca ggaacacatc gttcatgaac agctgattcc cctgcgccac
      901 aaacagcggc gtggtgttgc cgttctccgg gttaatcatc gagatacggt ctgcctgcag
      961 cagaatattg ctcaggggct ggccatcagc atcctcaatc cccgcaccaa taccggcaac
     1021 atacggaatg ccgtttttcg ttttctgcac cttcagcatg tacagcgcag ccaggtcgtt
     1081 atttgtgtct gtctgcaccc gctgtatctg ctgtatggtg gcactctggt cttccagggt
     1141 tttactgacc gtctgcgtga tttcattgcg ggtttctgtg atggtggtct tcatctccgc
     1201 catctcatcg tcaagctggc tgttatcaat cagctcccac atcccctgtg ccagatgtgt
     1261 cttcccgatt tctcctctga aaaagtccag atacccttcc gcatcattgc tcgcccggcc
     1321 actggcttcc acaaacgcag atttccccac caggttgacg ctgcgcacat aaaaccagaa
     1381 atccttcccc ggcttaatgt gcgggccgga gacactccac tggctgcctg tccccagata
     1441 acgggcagag gtttccacct gtgccatgtc cgtgatgcgt ttttctgaga accagaattc
     1501 agactgtacc gtcgggtcat acaccgccag caccggtact gctgttatct gataataccc
     1561 cggcgtcagc tcaatgctgg ccggtgctgc aggcgcatta atccggaacg tggtggtggc
     1621 aggtt
//
LOCUS       DAZUCE010000432         1624 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZUCE010000432 DAZUCE010000000
VERSION     DAZUCE010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.435"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..650)
                     /locus_tag="ACHY03_005169"
     CDS             complement(<1..650)
                     /locus_tag="ACHY03_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFS3908259.1"
                     /translation="MLKDGTGKPVPGCTIELKTRRTTETVIVTTVAYGQPGETGSYSM
                     DVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAV
                     AAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGE
                     TGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAATPKAVKA"
     gene            complement(814..954)
                     /locus_tag="ACHY03_005170"
     CDS             complement(814..954)
                     /locus_tag="ACHY03_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3908260.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHTSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            complement(1154..>1624)
                     /locus_tag="ACHY03_005171"
     CDS             complement(1154..>1624)
                     /locus_tag="ACHY03_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3908261.1"
                     /translation="NNVVIAENCTINGTLKAENIIGDLVKCAGVAFPVDGSYLANGTR
                     TLTVYDDHSFDRQIIIPPIIYVGSKQESRTSNDIWTECFLHVDQNGRRIYSGRSVTEP
                     GIFSGIIDMPAGHGHITLSFTVSSRRQNGSFGSSRISNLQAIVVKKNSAGISIR"
BASE COUNT          303 a          524 c          370 g          427 t
ORIGIN      
        1 gctttcaccg ctttcggggt ggctgccttc gtttcatcat cactgtccgt tgcgctgctt
       61 aactgcacaa ttcccttctg tgccgtcgtc gcatccggcc ctcccggctc gcctttttcg
      121 cccttctctc cccgttcacc tttcacaccc tgaagcccct gtggtcctgt ctcaccacgc
      181 tcaccctttg gcccacgctc gccggtatcg ccttttggcc cgggaatacc ctgcggcccg
      241 gtgtccccct tatccccctt cggtccccgc gcattctctg cccgtttttt tgcctcctcc
      301 gcactggctg cggaggcttc cgcacgtttc aggatttccg cggccaccgc ttccagctct
      361 gcaagggctt tcgggtaata ctgtgcgtcc tccaggtcca tcagaaattt attcagcgtt
      421 cccggtgcag agtccgcctt aaccagaatg tcacccacat atgacggcgc gtacccttcc
      481 gtgttcagcg tcacccggta cagacccggc tcaacatcca tactgtaact gccggtttcc
      541 cccggctgac catacgccac cgtggtgaca atcaccgtct ccgttgtacg gcgcgttttc
      601 agctctatcg tgcatcccgg taccggtttt cccgtaccat ccttcagcac accagatatt
      661 ctgactgtca tggatttccc ccataaaaaa accgcagtac cggtttcccg gcctgcggta
      721 aaatttgtgg tttgttggtg ctaaaacggt gccgtccggc tgaccaccct cagcaaccgg
      781 tcggcggggg atattctccc ccgccacggt ttcttactgc ttacactgta agaacgccgc
      841 aatctccgcg cccgctatcc ggaaccggaa ctcgcacagt gaagtgtggg tgatccagat
      901 aatgagcact accgtgatac aaatcacggt ggtttttaac ggtttttgcg acataaacgc
      961 ttgctccttt tacggagagg cgctaacctt ccacttgttg aaggtaggaa cgtcaggcct
     1021 cgggttaaac atgaatgttt gtccggggcc ttcgtctttc cggccttcag gtgttccctc
     1081 cggccatcag ccaaaggcac ccgcgcataa tgtacggttt ttgtctcctt ccggcaatcc
     1141 cggggcgcga tgttcagcgg atgctgatcc ccgcgctgtt tttcttcacc actatcgcct
     1201 gaaggttact gatacgtgaa ctgccaaaac tcccgttctg acgtcgtgaa cttacggtaa
     1261 aactcagggt gatatgacca tgaccggctg gcatatcgat gatcccgctg aaaattcccg
     1321 gctctgtcac tgacctgccg gaataaatcc ggcgtccgtt ctgatcaaca tgcaggaagc
     1381 actctgtcca gatgtcatta ctggtacggg attcctgttt tgacccgaca tagattatcg
     1441 gcgggattat aatctgccgg tcaaagctgt gatcgtcata caccgtcagc gttcgtgtac
     1501 cgttcgcaag gtaactacca tccaccggaa aagccacccc tgcacatttc acaagatcac
     1561 caataatgtt ctccgctttc agcgtgccat ttatcgtaca gttctccgct atcacgacat
     1621 tatt
//
LOCUS       DAZUCE010000433         1603 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZUCE010000433 DAZUCE010000000
VERSION     DAZUCE010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.436"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>1603)
                     /locus_tag="ACHY03_005172"
     CDS             complement(<1..>1603)
                     /locus_tag="ACHY03_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908262.1"
                     /translation="NDPKKMKRIFPLFIVSENINIHAEPDIEYFRVIDRAFDEVANYT
                     GRIFSYLRTHEPLKLTSCVNKQTLLSMGGYLNEWNVFDSLSRVRDFFRLSSAVFTKLD
                     NNIYSLEVDIFCLYRDYEIARNRLMMRASHLYSEVHEFSNKHFHLNSWVKDHMPSYLN
                     SDGVFSSFHLSELENMSPDDLHEEYGNISLFNWVHAYQCLVELSKEEMSKRFSSTKPI
                     PLQLDRWLIIKSRESWLSFFQRKGIAADAAKKLIDYFTFNSKSHDLNDCPFIPCMDGL
                     CLMPALIANSSVTRSLMSLFGSKKISQASKGRFHEQQFIKQVRDAGIKASPIDAHANY
                     QCDCVILLDDCLIFTELKSNGQPIYYGKYYQQVCNIVGDSSLIHDHNNKFMRSYFQQI
                     NRISEHYLNHLDVIIKEFELPSTWQPKGVYKLIVTTTMLGGKYHVDDTYVADKYALSS
                     FFQRIPGVIYQTNENGKMAKNIIDGFECCEGEITIDKFIDYLSSLPSINAVRKNIKKL
                     TYSVRFNEKLVHHPYYDSWAFGPYIR"
BASE COUNT          534 a          289 c          249 g          531 t
ORIGIN      
        1 acgaatatat gggccaaaag cccaagaatc ataataaggg tgatggacta gtttttcatt
       61 aaatcgaaca ctataagtta atttttttat atttttacgt acagcattaa tactgggcaa
      121 agaggaaaga taatctataa acttatcgat agtgatttcc ccttcgcaac actcaaaccc
      181 atcaatgata tttttcgcca tttttccatt ttcatttgtt tgatatatca ctccaggtat
      241 tcgttgaaaa aaactagata atgcatattt gtccgctaca tatgtatcat ccacatgata
      301 tttcccaccg agcatagttg tagtaacaat aagcttatac acacctttgg gttgccatgt
      361 tgatggtagt tcgaattctt taatgatcac atcaagatga ttaagataat gttcagaaat
      421 tcgatttatt tgttgaaaat aagaacgcat aaatttatta ttatgatcat gtattaatga
      481 gctatctcca acgatgttac acacttgctg atagtactta ccataatata ttggttgacc
      541 atttgatttt agttctgtaa aaatcaaaca atcatccagg agaatcacgc aatcgcactg
      601 ataattagca tgagcatcaa ttggagaagc tttaatcccc gcatcacgaa cttgttttat
      661 aaattgttgc tcatgaaatc gccctttact tgcttgagaa atttttttgg aaccaaataa
      721 tgacattaga gagcgagtca ctgagctatt tgctattaat gcaggcatta agcataaacc
      781 gtccatacag ggaataaatg ggcaatcatt aagatcatgt gattttgaat tgaaggtaaa
      841 ataatctatc agtttttttg ctgcatctgc tgcaatcccc ttacgttgaa agaatgataa
      901 ccaactctca cggcttttta tgatcagcca gcgatccaac tgtaggggta taggttttgt
      961 cgatgaaaat cttttgctca tttcttcttt agataactcg actaaacatt gataagcgtg
     1021 cacccaatta aataatgata tattcccata ttcttcatga aggtcatcag gagacatatt
     1081 ttccaactcg cttagatgaa atgagctaaa aacgccatca gaatttaagt aagatggcat
     1141 atgatccttt acccaactat ttagatgaaa atgtttgtta ctaaattcat gaacttcgct
     1201 atatagatgt gacgctctca tcattagtct atttcttgca atctcataat ctcggtataa
     1261 acaaaaaata tcaacctcaa gagagtaaat gttattatcc aattttgtaa atactgcact
     1321 acttagtctg aaaaagtctc ttactctgct taatgagtca aaaacattcc actcattaag
     1381 gtaccctccc attgaaagca aggtttgttt attcacacaa gaagttagtt tcaatggctc
     1441 atgagtccgt aaataagaaa atattcttcc ggtgtaattg gctacctcat caaatgccct
     1501 atcgataact cgaaaatatt cgatatcagg ttcagcatga atattaatat tttcagaaac
     1561 gataaataat ggaaaaatcc tcttcatttt tttaggatca ttg
//
LOCUS       DAZUCE010000434         1580 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZUCE010000434 DAZUCE010000000
VERSION     DAZUCE010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.437"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..350)
                     /locus_tag="ACHY03_005173"
     CDS             complement(<1..350)
                     /locus_tag="ACHY03_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFS3908263.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSL"
     gene            complement(359..1126)
                     /gene="sctT"
                     /locus_tag="ACHY03_005174"
     CDS             complement(359..1126)
                     /gene="sctT"
                     /locus_tag="ACHY03_005174"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFS3908264.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELSNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1128..1388)
                     /locus_tag="ACHY03_005175"
     CDS             complement(1128..1388)
                     /locus_tag="ACHY03_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFS3908265.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1398..>1580)
                     /locus_tag="ACHY03_005176"
     CDS             complement(1398..>1580)
                     /locus_tag="ACHY03_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFS3908266.1"
                     /translation="LPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTM
                     LSKGLILQYFDLSINP"
BASE COUNT          548 a          320 c          278 g          434 t
ORIGIN      
        1 ttcaatgaat caaacttaaa tttaacagct tcagttgcta attgcatttt actttgaaac
       61 caggagacaa gtattgttgt gaacatgcaa accagcaaaa aaggaatgat cgttttcaac
      121 caaccaatcc ccatagcttt aaaatagtcc caaatatctg gtttagcgtt atgatcaagg
      181 atgtattcaa gaatcgacat aatgtgatgc acatcaaaaa catatccgag atataaagta
      241 cccacaagca ttattacaga gacggttaag tctctacttt ttagaatttg ccctttttta
      301 gaagcatcct ggagtttctt ttgtgttggc ttttcagttt tattggccat gaaacactct
      361 atttctcaat gtatagttgc aattgttctg ggaaaaagga taacggcatt actcgttcag
      421 ccagtattgg gccaaaataa ataattaata ttaaaaatgc cagcgcactt ttgacagtca
      481 gtgaaatggc aaaagcattt agttgtgaag cataacgtgc cagcaatccc aggacagcct
      541 caccgcccag cataacggca atcacggggc ttgcgtaaac gatagtatga gtcattatgt
      601 tattaatata tgagaataaa ggctctaatt taggaaaatt aaaattacct gaaggccata
      661 aattataact ttgccaaaga gtttccagaa taaagttcat tccaccgtta gtaagataaa
      721 ccgcagcaga gaaaaggtta aataatcttg ctaattccga ggtatcgaca cctgtagccg
      781 gatccaaagt actacttaat gttgcaccgc gttggttatc gataaagcta cctatagcaa
      841 ggaaaatcca aaacggggat gctaataagc atgcaataaa cagccctatg ataatttctc
      901 tggcagcgaa caaagcaata tcgatttcat ttaagttcga taattcatag tggtaatgct
      961 gaactaatgc acatgatacc actattatta ttggatgacg gactactgtt ggtatattcc
     1021 cactggcgaa aaatggtaac aaatagaaag tgggtgccaa tcggcaaaaa ccgagcgctg
     1081 tagcagccag cagactatgc aactgataga gtatcgcctc acccatgcta tcctctcgcg
     1141 aatgctaaat tcagcatttc aataccaaaa gaatataatt tttcgccata ccatccgctc
     1201 attaaaaaaa agcaaatact tacacataat aatttcacgc caaatggcaa cgtctgttct
     1261 tgcaactgtg ttaccgtttg aaaaagccct actaataaac caacaaatgt cgcaactgcg
     1321 ataggtcctg cagacataac taaaatcaga tacagcgccc tattccctgc aaaaacaata
     1381 tcgtccatat tttttcctca gggattaatg gataggtcaa aatattgtaa aatgagtcct
     1441 tttgataaca ttgtccaacc gtccatagcg acaaacagaa ttaattttat cggtgtagat
     1501 atggttaccg ggctcatcat catcatacct aacgttaaca aaacgctgga tataaccaga
     1561 tccactacaa caaagggtaa
//
LOCUS       DAZUCE010000435         1577 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZUCE010000435 DAZUCE010000000
VERSION     DAZUCE010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.438"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..165
                     /locus_tag="ACHY03_005177"
     CDS             <1..165
                     /locus_tag="ACHY03_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFS3908267.1"
                     /translation="DVRTMVALGLVMDFDARNVTTFEGERHNALHDARYQAKYVSAIW
                     QKLFPNQADF"
     gene            224..427
                     /locus_tag="ACHY03_005178"
     CDS             224..427
                     /locus_tag="ACHY03_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFS3908268.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCRNTLPPREAEPDFGAI"
     gene            427..1452
                     /locus_tag="ACHY03_005179"
     CDS             427..1452
                     /locus_tag="ACHY03_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3908269.1"
                     /translation="MGRRRKNPEHEKLPPKVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNHHDHAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVL
                     ADSVKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPVPVFIAMEIAYLCAARISDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWTPRLQSVIEKAKQLPKSAYVISNQYGNRYMYKGFNEMWVEARNLAGQIS
                     GIATDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKISPTLDVPLPQN
                     IPTKYSK"
BASE COUNT          509 a          327 c          369 g          372 t
ORIGIN      
        1 gatgtaagaa cgatggtcgc tctggggttg gtgatggatt tcgatgcccg caacgtcacc
       61 acatttgagg gtgaacgcca taatgccctg cacgatgcgc gttaccaggc aaaatacgtt
      121 tcagctatct ggcaaaaact gttcccaaat caggctgatt tttaatgttc aaccctgatc
      181 gccgctaacc gcatatagtt agcggcggtt atgagatata gctatgagca gcttattttt
      241 aaccgaagat gaattgctaa tattaacggg ctgcaaatat gcaagccacc agcgcaactg
      301 gttaataaaa aatgggcttc cgttctatac caatcgtagt ggaaaaccga tcgtcagtcg
      361 ggaactgttt acctgtagaa acactttacc accacgtgag gctgaacctg atttcggtgc
      421 gatctaatgg gaagacgaag gaaaaatccc gaacacgaaa aattaccgcc aaaggtatat
      481 cccaataaat atagttatgt atggaaacca acatccagag aatctgtaac cttaactgca
      541 atcaaggatg gtttagccgc attatggaaa aaatatgaag aaacggttaa ccatcatgat
      601 cacgcaatga catttgggcg tttgtgggaa aaatttctcg ccagcgccta ttacagcgat
      661 cttagtccca gaacgcaaaa agattatctg caacatcaaa aaaaactgct ggccgtattc
      721 ggtaaggtgc tggccgattc tgtaaaacca gagcacatca gacgatacat ggacaaaagg
      781 ggcgagcaga gtaaaacgca ggcaaaccat gaaaaaagca gcatgtcacg cgtttacagt
      841 tgggggtatg aacgagggta tgtaaaggct aatccttgtg ccggtgtaag taaattcaag
      901 gccaaaaacc gagagcgcta tgtgactgac aaagaatacc aggccgtatt aagcgtggct
      961 cctgtgcctg tttttatcgc aatggaaatt gcatacctgt gcgcagcaag gatttccgat
     1021 gtgttgtctc tgaaatggga acagatcgga aacgacggga tctttatcca gcaaggtaaa
     1081 acaggaaaga aacagataaa agcctggact ccccgtctgc aatcagttat agaaaaagct
     1141 aaacagctac ccaagtcagc ctatgtaatc agcaatcagt acggcaaccg ttacatgtat
     1201 aaaggattta atgaaatgtg ggttgaggca agaaatttgg ctggacaaat atcagggatc
     1261 gcgacggatt ttacattcca tgaccttaaa gccaagggga tatcagatta cgaaggtagt
     1321 agccgggata agcaactttt ctctggtcac aagaccgaag gacaggtgct aatctatgat
     1381 agaaaagtaa agatctcacc cacactggat gtaccattac cccagaatat tccaacaaaa
     1441 tattccaagt aattccaagt gtgattttca tcattgactt aatgatgtgt aagtgattga
     1501 attctggcgg agagaggggg atttgaaccc ccggtggagt tgcccccact ccggttttcg
     1561 agaccgataa taccgaa
//
LOCUS       DAZUCE010000436         1572 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZUCE010000436 DAZUCE010000000
VERSION     DAZUCE010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.439"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..368)
                     /gene="traM"
                     /locus_tag="ACHY03_005180"
     CDS             complement(<1..368)
                     /gene="traM"
                     /locus_tag="ACHY03_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFS3908270.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSELERFFQ"
     gene            691..1290
                     /locus_tag="ACHY03_005181"
                     /pseudo
     CDS             691..1290
                     /locus_tag="ACHY03_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
BASE COUNT          489 a          334 c          310 g          439 t
ORIGIN      
        1 ttttggaaaa atctttccag ctcagatgac accttctccc tgatatcttc gaccatattt
       61 gcatattcaa actttggatt tcctgataca tgaggactga gtgactcaat acctaaaatc
      121 ttggcaactg ttgactgtgt ttttacaacg cattcaagaa gcaatttatt aaactcagtc
      181 tgattaaatg cagactcttt acgctccatc tgagcctcat atacacgaag ccccagttca
      241 agaagcattg aagctgttcc cgaaacactt atatctttat ctcttgcccc ctcctgacga
      301 cgctgttcaa ctatcgcatt tactttatca tagacatcat tactgatata caaaattact
      361 ctagccataa gataaaacct ttcattatta agcaatgaac ttttcactat aaatatgcat
      421 atagtgttta caagtaaaaa agacactcct agcagcgcct ctaggatcat cctataaaaa
      481 aatgcgatcc ggcgctaggg gcgtccctaa tatatatcaa tgtttttcat gaaaattgtc
      541 agtactgatc ctaataagag tcgctatagg gtcgtaacag gatcgccaac gactctctat
      601 ttaataattc agaattatta aatataaata gcatttatta attacatgat ttaaaacgca
      661 aatcagcaaa aacttgtttt tgcgtggggt gtggtgcttt tggtggtgag aaccaccaac
      721 ctgttgagcc tttttgtgga gtgggttaaa ttatttcctg atgacgtcac cagaggtgag
      781 aaaatgagaa aatggcttct gacgatctgc ctgatgttta taaatgtgac ctgcaaggct
      841 ggtgattgct ttgatctggc tggcagagat tataaaattg accctgactt gctgagagca
      901 atatcatgga aagaatcccg taaccgggtt aatgccatcg gtattaatcc ggttacggga
      961 tatggcagtg gacttatgca ggtagattcc cagcatttta acgaactggc ccgctacgga
     1021 attaaaccgg aacatctgac agcagatccc tgtctgaaca tttataccgg tgcttattat
     1081 ctggctatag cctttaaaaa atggggagtc tcctgggagg ctgttggtgc atacaacgca
     1141 ggattcagga aaaccgaacg tcagaaccag cggcgtcttg cctatgcatc agatgtttac
     1201 cggatttaca gcgggataaa aaacagcaag gggatccagc ttcgtgcatc tgaaaaaccg
     1261 ctttcaaaaa tgaacagctc gcagaagaat tattttgata tacattttaa aaaaaccggt
     1321 tatgccgcaa acgcggcatg gaaggccaca cacaaaaata tcatatgagt gataatgctg
     1381 actggccgaa gccccgcaaa gcgggaaccc atcttatata tatccggtaa ctgcgcagcc
     1441 tcctgccaga ccatttttcg cgggcacaat atttacagac taccgaaaag ccagttgatg
     1501 aaattgaggc atctcagacg acatatcaat gccgcataat gataatataa gtaaagcccc
     1561 gaaaaaattt tc
//
LOCUS       DAZUCE010000437         1570 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZUCE010000437 DAZUCE010000000
VERSION     DAZUCE010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.440"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(53..1450)
                     /gene="ccp"
                     /locus_tag="ACHY03_005182"
     CDS             complement(53..1450)
                     /gene="ccp"
                     /locus_tag="ACHY03_005182"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFS3908271.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT          403 a          422 c          325 g          420 t
ORIGIN      
        1 tattatcgta ataaaaatcg cccaaacatt cctgttcggg cgatcaaatt aattattgtt
       61 tatcctgcat atacggcgtg taaaccccgt tcagactgtg caggaaagct acgatatcat
      121 ccacatcctc ctgcggcagc tctttgccta cctgatagcg cagcatcagt ttcaccgccc
      181 cgtccagcgt cggcacgtca ccgcgatgga agtacggtgc cgttaaagca acgttacgta
      241 aaccgggtac tttctgacgc aatttatcac gctcttcttt agtcacattc atacgaccaa
      301 tatccgccgc cgtaatttcc ccaaagttaa agtctttttt cagccccaac ggttcgaagg
      361 aacgtccgcc gagaataatt ccaccatgac aagttgcaca tttattatct ttaaataatt
      421 gatagccttt tttctgttgc gccgtcagcg cattctcatc tccacgcaac catttatcaa
      481 atggggaatc cggcgtaatt aatgttttct caaattcagc aatggcatca gtaatatttt
      541 cgccactgaa accttgcgga tagacttcga ggaactgcgc tttaagctgc ggatcttttt
      601 ccagcttagc aataatttcg tcccaggatt ttgacgccat ttcaatcggg ttcaacggcg
      661 gtccaccagc ctgatcctgc aatgttgccg cacgaccatc ccagaactgc tcaacgttaa
      721 atactgagtt aaataccgtc ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg
      781 atgtttttct gccatcgacg ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg
      841 tgctatcagc cgataaacgg ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg
      901 catcggtagg cagtttttgc gggatgggct gcaccggttc attgcgatgc tccggagcag
      961 tatcattgct ggcgtaatat tccgcgcgct gttttgcaat ccaggccagt atttccgccc
     1021 gctcttcatc actcacctta cccgcccagt gtagcgcggt ataacgcgtt ggtggcatag
     1081 tttcatactg catcacccat tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag
     1141 ccagcagtgc cgcacgcacg gcctcgaggt taaaagattt atatccaagc ttaatgtcgt
     1201 aatccatcaa ctgtttcgcg ccaggaatat aataataggc gggtaattct gccgaaggcg
     1261 tatggcaata gtcgcatcct ttttcgcgga gaaagcccaa aaccttatta ttttcactaa
     1321 cagcagatgc ctgaacatcg gcctgtttac tgcgtttatt atcgtggtac cacacataac
     1381 cagataaccc taaatagcaa atcgcgacgc cagccaggcc gatcgcggta atacgtgaga
     1441 ccattttcat tattcttccc tcacggtggt gagttattgt gacccagaaa attttccaga
     1501 atgctgcaaa cgtctctgcg agacaaagcc atcataaaaa acagtgagat acgctttttg
     1561 ataggaatca
//
LOCUS       DAZUCE010000438         1563 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZUCE010000438 DAZUCE010000000
VERSION     DAZUCE010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.441"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..732)
                     /locus_tag="ACHY03_005183"
     CDS             complement(<1..732)
                     /locus_tag="ACHY03_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908272.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLTCEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKNRMAKLFNQSDTSVRNKYRPAFDKWNELV
                     GKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LLEKRLADGRKIENLFDQQGYFSCDS"
     gene            complement(745..1563)
                     /locus_tag="ACHY03_005184"
                     /pseudo
     CDS             complement(745..1563)
                     /locus_tag="ACHY03_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          423 a          340 c          283 g          517 t
ORIGIN      
        1 tgaatcgcaa ctaaaatatc cctgttgatc aaaaagattt tctatttttc taccatcggc
       61 gaggcgtttt tcaaggagtg tttgcaacat cgagttgcca aggacaatgg atatcgtgcg
      121 atctttcgca gatttacacc ccatcacaac ggcaacattc gtatccccct caggagtttc
      181 atttatcgct gtcgctaaaa tggtggtcaa acacgcaaac ggaactgtat ttctttcgtg
      241 atttaattta cctaccagtt cattccattt atcaaaagcc gggcgatatt tgttgcgcac
      301 agaagtatcc gactggttaa acaatttagc catgcgattt tttatcatct cgatatccgg
      361 cgaaccagaa agactttgcg cataatacgg tcctgatgat gttgcataca ttaaagccgc
      421 gcccacacct gtcgccatga tgggcgcagc cacggttttg gcaagggcga cgctgcgatg
      481 gctatgcgct tcacaggtta aaggaacata ttccactgac tgcggtaatt ccttacatgc
      541 ctccctcatc attgccgtat gctttacaaa caacggtttt tcactgccca aaaaaacttt
      601 ctgcttaatt gcactaagaa aacgtaaatc aaaaatgatt gtttttggtt tattattttt
      661 tggcacattt cctattatcc cgctgagaac ctttcttgct gcatggagat ttttttgcac
      721 agcacataac acaattgacg aagccttctc tactgcgtca ttgattttaa ttttaaaatc
      781 agcatgatat acgttgacgg gcaatataag tatgtctggc gctacatctt tggctgagta
      841 tttattggtg atcgacggtc gtgaatccac gagaaaactt agctgcaaat tatgctctat
      901 attaccagct gtttttgtgg tgtgattaaa catagagatg ccacctctca ttctttcaca
      961 catatcctca actttttcac ttcgttcttg tggcaatttg gctttaacgt taatttgatg
     1021 atttttaccc gtgcattcct taactacaag gggaatgtta tctaaagtgc catacaggct
     1081 aataacgtca ccaggatttt tatcatgtaa gtaagtttga ttaatactcg aagcattcac
     1141 aaagaggtca atattataac tgtttataac ttcacctaca tcaaatgtgt agtgcttact
     1201 ctggaggctg ccatccttat ttaattgtat atcaatcaat atatccccgg actttgtcac
     1261 catatctttg ccataaacca tttgcatagc catttcgagg agatttttat ctaactgatt
     1321 gcgggcgttg agtaagcgac taccaaataa tttatatact gttcccgtca ttgccagggc
     1381 tgcgctttct ggtactttgc gtttccacag tgaggtttct ctatttttaa acaggtcaca
     1441 ttcattatta ctcgacaaac catatttcag ttcgttaaca caatggaaca gttgttcctc
     1501 accttccatt ttaaggcgat gtgaaaaatc atttaatagc gtttgcttcg gtgattcatt
     1561 act
//
LOCUS       DAZUCE010000439         1549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZUCE010000439 DAZUCE010000000
VERSION     DAZUCE010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.442"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..941
                     /locus_tag="ACHY03_005185"
     CDS             <1..941
                     /locus_tag="ACHY03_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS3908273.1"
                     /translation="KNMFHCFHVFLLKLCSIESIPLPNADYAFFDDEMPFTLTDEQIE
                     NISFLNAYHKEKKGNNDIVTFDFMSADHHYNFSTTIALTNDSFHISSINNHNSQVIFD
                     ENIHLNPYELPESSQWCYQLIKNMISLHCRYNNNFKIKKTFCFDFKNKSFCNARLQYI
                     TFIACLFSHANMKYSTFHDVNLDMCEIKNCNFDNSEMNFISCVGTNFSGSTFNNVKTT
                     TAQLIKTPTKWTNNTLKYWFSSCNKRNIIFTFNTISDRNMKLKGIKDILLSLVDQKVN
                     IYSVRQELLDFLNNDLYKNDGEILSYKESIMLFCAV"
     gene            1135..1239
                     /locus_tag="ACHY03_005186"
                     /pseudo
     CDS             1135..1239
                     /locus_tag="ACHY03_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          521 a          270 c          239 g          519 t
ORIGIN      
        1 ataaaaacat gtttcattgt tttcatgttt ttttattaaa actatgctca attgagagta
       61 ttcctctccc aaatgccgat tacgctttct ttgatgacga aatgccattt acattaactg
      121 atgaacagat cgaaaatatt tcttttttaa acgcatatca caaagaaaag aaaggaaaca
      181 acgacattgt tacatttgat ttcatgagcg ctgatcacca ctataatttt agcacaacca
      241 tagctttaac aaatgatagc ttccatatat cttcaataaa caaccataac tcacaggtta
      301 tattcgatga aaacatccat ttaaatccct atgaattacc agaatcatca caatggtgtt
      361 atcagttaat aaaaaacatg atttctttac attgcagata taataacaat ttcaaaataa
      421 aaaaaacctt ttgttttgat tttaaaaata aatctttttg taatgccagg ttgcaatata
      481 taacgtttat tgcatgttta ttttcacatg caaacatgaa atattcgaca tttcatgatg
      541 ttaaccttga tatgtgtgag ataaagaatt gcaattttga caattcagag atgaatttca
      601 tttcgtgcgt cgggacaaat tttagcggtt ctacatttaa caatgtaaaa acaacaacag
      661 cgcaactaat taagacacca acaaagtgga cgaataatac tctaaaatat tggttctcca
      721 gttgtaataa acgtaatatc atcttcactt ttaatacaat atctgataga aatatgaaat
      781 taaaaggtat caaggatata ttactgtcgt tggttgacca aaaagtcaat atttatagtg
      841 taaggcaaga gcttctggat tttcttaata atgatttata taaaaatgat ggagagattc
      901 tctcttataa agaatcaata atgttgtttt gtgctgtgta gccgaaaaat agatgattca
      961 gattaactac cgcactctat gttgtgttta tatccatgtc aggtaacagg tcatttgtga
     1021 atcctgacac cagactatga ctatcgccca gggataatcc acaaccgctg gaagtagtct
     1081 catttcagca ggataaaacc gtagagtgcg attgcgatga ataataaata caggcttatt
     1141 attccagtct gttttattgc gttggttttt tcaaatggct cggttagtaa aatgcaccat
     1201 tcgctcggaa aactcaacat gttgcactct tatacgccgt ctggaaaagg cgtaggagtg
     1261 gtataaccat ttaatcgtct cccaacgtca acgcttacac atcagcgaca ctggtttaat
     1321 taaagcatac cagatttctg atgagtaagc gtcttttcgt ataacctcat tgtcacagga
     1381 acgacttgat ccgtttattt attcgccacg ttattattcg gtatcaattt tcttaatagt
     1441 ttccaccata acttgatttt cttttagcca acgactgata cctagaatta catcattaag
     1501 gctattttct tgttgaaact ttatcttgaa tctctctgtt tcagaggaa
//
LOCUS       DAZUCE010000440         1544 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZUCE010000440 DAZUCE010000000
VERSION     DAZUCE010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.443"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1014
                     /gene="tssC"
                     /locus_tag="ACHY03_005187"
     CDS             <1..1014
                     /gene="tssC"
                     /locus_tag="ACHY03_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFS3908274.1"
                     /translation="PIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGPAFF
                     RKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDTVPV
                     RSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGHQAGGAVQDLPIH
                     LYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPAQYD
                     TPDATANSRINARLPYIFLLSRIAHYLKMIQRENIGTTKDRRLLELELNTWLRGLVTE
                     MTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPK
                     AKA"
     gene            1030..>1544
                     /gene="tssK"
                     /locus_tag="ACHY03_005188"
     CDS             1030..>1544
                     /gene="tssK"
                     /locus_tag="ACHY03_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3908275.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSDRSLVDIVLA
                     LPLLNANGGNLDDGRESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVR"
BASE COUNT          321 a          373 c          453 g          397 t
ORIGIN      
        1 cccattgcag ccctgatttc tgcctgggag tttgatgcgt ctgcgcagga tgtggcgctg
       61 ctgaaaaata tttcccgggt ggctgcgtcc gcgcatatgc catttattgg ttcagttggc
      121 ccggctttct tccggaagga tacgatggaa gaggtggccg ccattaagga tattggcaat
      181 cactttgagc gtgccgagta catcaagtgg aacgctttcc gtgaaacgga tgatgcccgc
      241 tatattggtc tggtgatgcc acgtgttctg ggacgcctgc cgtatggtcc tgataccgta
      301 cctgttcgca gctttaacta tgttgaagag gtgaaaggtc cggatcatca taagtatctc
      361 tggacaaatg cgtcttttgc ttttgcggcc aatatggtgc gcagttttgt caccaacggc
      421 tggtgtgttc agatccgcgg tcatcaggcc ggtggtgcgg tgcaggattt gccgatccat
      481 ctgtatgatt tggggaccgg taatcaggtc aaaataccgt cagaggtgat gattccggaa
      541 actcgggagt ttgaattcgc taatctgggc tttattccgc tctcttacta caagaaccgg
      601 gattatgcct gcttcttctc cgctaactcc acccagaaac cggcacagta cgatacgccg
      661 gatgcgacag cgaacagccg tataaatgcc cgtcttccct atattttcct gctgtcccgc
      721 attgcgcatt acctgaagat gattcagcgg gagaatatcg gtaccaccaa agatcggcgt
      781 ctgctggagc tggagctgaa tacctggctc cggggcctgg tgacggagat gaccgatccg
      841 ggcgatgagc ttcaggcatc gcatccgctg cgtgacggaa aagtggtggt ggaagatatt
      901 gaagataacc cgggattttt ccgcgtgaaa ctctatgctg taccacattt ccaggtggaa
      961 ggaatggacg tcagtctgtc actggtttcg cagatgccaa aggcaaaagc gtaaggcagg
     1021 cgtcgggaaa tgaagattta tcgtccactg tgggaagacg gggcttttct gatgccccag
     1081 cagtttcagc agcaggctgc ctgggatgtt catctggcag acagcgttgc cagaatgggg
     1141 ctggcacatc cctggggcgt tgtggccgca gagtttgatg actctctgtt acctctttca
     1201 cggttgaatg ccacccgtct gattgttcgt ttcccggacg gaacgcttat tgatacggaa
     1261 cgtgcggata atctgcctcc ggtctgtgat ttatcgacgg tttctgatcg ttccctggtg
     1321 gatattgtgc tggcgctacc gttactgaat gctaacggtg gcaaccttga tgatggccgt
     1381 gagagcgagc gtccgcgtcg ctggaaatca gaaagggtga atgtgcagga gctggccggg
     1441 catgagcaaa gcgaagtggc ggtattgcgg cataacctga ctctgcgcat ggctcatcag
     1501 gaaaatgctg cctggctgac ctgtcccgtt acccgtcttg ttcg
//
LOCUS       DAZUCE010000441         1544 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZUCE010000441 DAZUCE010000000
VERSION     DAZUCE010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.444"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1014
                     /gene="tssC"
                     /locus_tag="ACHY03_005189"
     CDS             <1..1014
                     /gene="tssC"
                     /locus_tag="ACHY03_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFS3908276.1"
                     /translation="PIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGPAFF
                     RKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDTVPV
                     RSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDLPIH
                     LYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPALYD
                     TPDATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGLVTE
                     MTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQMPK
                     AKA"
     gene            1030..>1544
                     /gene="tssK"
                     /locus_tag="ACHY03_005190"
     CDS             1030..>1544
                     /gene="tssK"
                     /locus_tag="ACHY03_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3908277.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVR"
BASE COUNT          322 a          375 c          450 g          397 t
ORIGIN      
        1 cccattgcag ccctgatttc tgcctgggag tttgatgcgt ctgcgcagga tgtggcgctg
       61 ctgaaaaata tttcccgggt ggctgcgtcc gcgcatatgc catttattgg ttcagttggc
      121 ccggctttct tccggaagga tacgatggaa gaggtggccg ccattaagga tattggcaat
      181 cactttgagc gtgccgagta catcaagtgg aatgctttcc gtgaaacgga tgatgcccgc
      241 tatattggtc tggtgatgcc acgtgttctg ggacgcctgc catatggtcc tgataccgta
      301 cctgtccgca gctttaacta tgttgaagag gtgaaaggtc cggatcatca taagtatctc
      361 tggacaaatg cgtcttttgc ttttgcggcc aatatggtgc gcagttttgt caccaacggc
      421 tggtgtgttc agatccgcgg tcctcaggcc ggcggtgcgg tgcaggattt gccgatccat
      481 ctgtatgatc tgggaaccgg taatcaggtc aaaattccgt cagaggtgat gattccggaa
      541 actcgggagt ttgaattcgc taatctgggc tttattccgc tctcttacta caagaaccgg
      601 gattatgcct gcttcttctc cgctaactcc acccagaaac cggcactgta cgatacgccg
      661 gatgcaacgg cgaacagccg tattaatgcc cgtcttccct atatcttcct gctgtcccgc
      721 attgcgcatt acctgaagat cattcagagg gagaatatcg gtaccaccaa agatcgccgt
      781 ctgcttgaac tggaactgaa taactggatc cggggtctgg tgacggagat gaccgatccg
      841 ggtgatgaac tgcaggcctc acatccgctg cgtgacggaa aagtggtggt ggaagacatc
      901 gaagacaacc cgggattttt ccgcgtgaaa ctctatgctg taccacattt ccaggtggaa
      961 ggtatggacg tcagtctgtc actggtttcg cagatgccga aggcaaaagc gtaaggcagg
     1021 cgtcgggaaa tgaagattta tcgtccactg tgggaagacg gggcttttct gatgccccag
     1081 cagtttcagc agcaggctgc ctgggatgtt catctggcag acagcgttgc ccgaatgggg
     1141 ctggcgcatc cttggggcgt ggtggctgca gagtttgatg actctctgtt accgctttca
     1201 cggttgaatg ccacccgtct gattgttcgt ttcccggacg gaacgcttat tgatacggaa
     1261 cgtgcggata atctgcctcc ggtctgtgat ttatcgacgg tttctgagcg ttccctggtg
     1321 gatattgtgc tggcgctacc gttactgaat gctaacggtg gcaaccttga tgatggcagt
     1381 gagagcgagc gtccgcgtcg ctggaaatca gaaagggtga atgtgcagga gctggccggg
     1441 catgagcaaa gcgaagtggc ggtattgcgg cataacctga ctctgcgcat ggctcatcag
     1501 gaaaatgctg cctggctgac ctgtcctgtt acccgtcttg ttcg
//
LOCUS       DAZUCE010000442         1541 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZUCE010000442 DAZUCE010000000
VERSION     DAZUCE010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.445"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..579)
                     /gene="tssG"
                     /locus_tag="ACHY03_005191"
     CDS             complement(<1..579)
                     /gene="tssG"
                     /locus_tag="ACHY03_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFS3908278.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYPGMGFPASEIKDAVIPEESHLPPTVQVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEPGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRT"
     gene            complement(534..>1541)
                     /gene="tssF"
                     /locus_tag="ACHY03_005192"
     CDS             complement(534..>1541)
                     /gene="tssF"
                     /locus_tag="ACHY03_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS3908279.1"
                     /translation="FMFVHLNGLENVSLPAGISGFDLEVVLSQSWPADLPVTDDALCL
                     HCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTEIYSVDAVTGSGRTGSAEYVP
                     FTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLILGGQRWEADRMPERETLSLRI
                     TGTNGQLPRRALQSTLLDRCEQVLQTPVSVHNLCKPTLPVYPPTEDCFHWRVMSHLGT
                     GFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQHHRIQRFEKGFLLRGLDVEV
                     TLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTLIVQPEGKCIRWKENHNPRLP
                     G"
BASE COUNT          395 a          440 c          380 g          326 t
ORIGIN      
        1 tgttcttccg ggtaacaaca tcaggggcaa taacgccaga aaacgcgata ccggcgtggc
       61 aatattctgt gcacatccgg ggatccccag tctggccagg ccaagcagat actgagacgt
      121 tttatcctgc ccacccggct caaaggtggc cgggtacgaa tacttacgcc agatacggta
      181 atactgggtg atcagacgat gactgaatat atcgagaaaa tccgctgcgg cctcatggcc
      241 ttcccgctgc tgtgcaatat cactgatata gtgtgccggc aacggagacg tgaccccgta
      301 aagtcccata aacgtcacct gtacagtggg tggcagatgg gattcttcag gaattacagc
      361 gtcttttatt tcactcgcag gaaaccccat tcccgggtat ggacaaaagc ggaccgcttc
      421 ctgacgggcc tgccagccac tgccaatggg aggctggtct ggctggcttt gctccagcaa
      481 ctgacagaag cggtaaaaat taatatacgg aagctgtttc cggtaacggg taattaaccg
      541 ggcaggcgcg gattgtgatt ttctttccac cggatgcatt ttccttcagg ctgaacaatc
      601 agtgtgagct gattgaactg attcatatcg gcgtacaggg ccaggaaacg gttgagcatt
      661 tcgccaaaca ggtgaatatc tccctctccg gcaaacccgt tgccgtcaag agtcacctcc
      721 acatcaagcc cccggagcag aaaacccttt tcaaaacgct gaatacgatg atgctgtaca
      781 gccagaatgg catcaagccg acggtgatta agttcatcgt cccgccagtt ataaagcgcc
      841 agcgtgccac gcagcacttc cgcactgctc agcatgttaa gaaatcctgt ccccagatga
      901 ctcatcaccc gccagtgaaa acaatcttct gtcggcggat aaaccggtag cgtgggttta
      961 caaagattat gtacactgac cggggtctgc agtacctgtt cacaccgatc aagcagcgta
     1021 ctctgcaacg cacgacgggg aagctgacca ttggtacctg taatacgtaa tgaaagcgtt
     1081 tcccgctccg gcatccgatc cgcctcccac cgctgaccgc caaggataag ccatgtgtca
     1141 tacatccccg tgacaccccg tttcacccgg gtatgataat aacgttcggg tgcatcatgg
     1201 cgcagcattc ccccacggtg acggaagctg gtaaaaggca cgtactctgc actgccggtc
     1261 cgcccggagc ccgtcaccgc atccactgaa tatatctccg tatacccatc ctgcagacgc
     1321 ttcgggcgca gcaggtattc gctctccagc ccattgatga tgagtggatc ggcttcaagc
     1381 gtaaaaaggt taatcaccgg cacacaatgc agacacaggg catcgtctgt cacaggtaaa
     1441 tctgccggcc aggactgact cagcaccacc tcaagatcga atccggaaat acctgccggc
     1501 agagaaacat tctccagtcc gttcagatga acaaacataa a
//
LOCUS       DAZUCE010000443         1536 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZUCE010000443 DAZUCE010000000
VERSION     DAZUCE010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.446"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(165..377)
                     /locus_tag="ACHY03_005193"
                     /pseudo
     CDS             complement(165..377)
                     /locus_tag="ACHY03_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            527..1195
                     /locus_tag="ACHY03_005194"
     CDS             527..1195
                     /locus_tag="ACHY03_005194"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFS3908280.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1140..1277)
                     /locus_tag="ACHY03_005195"
     CDS             complement(1140..1277)
                     /locus_tag="ACHY03_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFS3908281.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1250..>1536)
                     /locus_tag="ACHY03_005196"
     CDS             complement(1250..>1536)
                     /locus_tag="ACHY03_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS3908282.1"
                     /translation="SPEGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHI
                     APLQDAVDMEIATEKETLLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
BASE COUNT          431 a          360 c          290 g          455 t
ORIGIN      
        1 ttgctcccaa tgcaaaacat atattgcatt gggagcatat tcatattttt gttattcttg
       61 tatgaaatcg tctcatgtac acggtccttt tttcgccgaa aactgatatt actttaatca
      121 aaagttgacg tgccagattc gaagtaaatt tataaaatat caaatcaata tacattttgc
      181 accttcgaga tagtaatggc tgttactgta acatgagtta caaacagctt catacccaga
      241 gctactgatg tatcaactcg attcaacaat acccgatatt tattccatgc ctccagcaac
      301 gatctttctt cctccgttgc gatttccaga tctacagcat cctgcagtgg cgcaatatac
      361 tcactgaatt cctggatgta gaactgtgtg gtgacggtct tccagccatt cggctcctgc
      421 tgtatcgaag cataccatgc tatttcaata tcgctatgct gcggcagcat ttaacccctt
      481 gtaattcatc gccataattg atttaattca caaataaaac tataacatgg tgaaattaat
      541 gaaaaaaaac acagatgatg gggctaaaat ttacacacca cttaccctaa agctttatga
      601 ctggtgggtt ttgggagtat caaatcggct tgcatgggga tgtcctacaa aggaacacct
      661 tcttccacac tttctggaac atttaggtaa caaccatctg gatattggtg ttggaactgg
      721 gttttacctt actcacgtac ctgagagtag tctgatatct ttaatggatt tgaacgaagc
      781 tagcctgaac gcggcatcta caagggctgg ggaatcaaaa attaaacata aaattaacca
      841 tgatgttttt gaaccttatc ccgcggcgtt acatggtcaa tttgattcca tttccatgtt
      901 ttaccttctt cactgcctgc ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc
      961 ggcgcaggcc ttaactgacg atggaactct atacggagcc acaattcttg gcgatggagt
     1021 tgtgcacaat agcttcggtc aaaaactgat gcgcatttac aatcagaaag gcatcttttc
     1081 aaacacaaaa gattccgaag aaggcttaac acatatactc tcagagcatt tcgagaatgt
     1141 taaaaccaag gttcaaggta ctgtagtaat gttttccgct tcagggaaaa aatagcatcc
     1201 aaccgcagca cgttcttgct taagacgtgc tgcggcataa tcccaatgat tactccctga
     1261 cagggttcgt aggccactca atatcaggtg cagttgatgt atcaacacgg ttcagcaaca
     1321 cccgatactt tttccaggct tccagcaaca aggtttcttt ctccgttgcg atctccatat
     1381 ccacagcatc ctgaagcggc gctatatgct cactggctac ctgcatcagg ctgttttttg
     1441 tttcttccgc ctcccggatc cggaacagtt tttctgcttc cgtatccttc acccaggctg
     1501 tgccgttcca cttctgaaac tccccttccg gggata
//
LOCUS       DAZUCE010000444         1525 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZUCE010000444 DAZUCE010000000
VERSION     DAZUCE010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.447"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1404
                     /locus_tag="ACHY03_005197"
     CDS             <1..1404
                     /locus_tag="ACHY03_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFS3908283.1"
                     /translation="LLPSLVREYRYDSAGNVSGVTSREDYGRETQREYRLDRNGQVTA
                     VTASGTGLGYGEGDESYGYDSCGYLKAQSAGGHRISEETDQYAGGHRLKQAGNTQYDY
                     DAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEYRHDASGRRTEKRCD
                     RKKIRFTYLWDGDSIAEIREYRDDELYSVRHLVFNGFELISQQFSRVRQPHPSVAPQW
                     VTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPADTGYPDPRGELDPEA
                     DPGLLYAGQWQDTESGLCYNRFRYYEPETGMYLVSDPLGLLGGKQTYRYVPNPLGYID
                     PLGLAKTSVPAEKISLSDKARDLFRQGKVREALDVHYEDLVRRKLGGISQEIAGREYD
                     VVTDKIIAQVKRTYSSIDNPKNFLSKSTRTQIKKTIELAEEQGKEAQFWFKYGVSPKV
                     REYIESKGGKVILGMGN"
BASE COUNT          424 a          346 c          462 g          293 t
ORIGIN      
        1 ctgctcccct cactggtacg ggagtaccgt tacgacagcg cgggtaacgt atcaggcgtc
       61 accagccggg aagattacgg acgggaaaca cagcgggagt accggcttga ccggaacggc
      121 caggtcacgg cggtgacagc ctcaggcacc ggactgggct atggcgaagg cgatgagtcc
      181 tatggctatg acagttgtgg ctacctgaag gcgcagtctg caggcgggca ccggataagt
      241 gaagagactg accagtatgc cggaggccac cggctgaaac aggccggaaa cacgcagtat
      301 gactatgacg ccgcaggccg gatggtcagc cgcaccagac accgtgacgg ctatcgcccg
      361 gaaacagagc ggttccggtg ggacagccgg gaccagctga ccgggtattg cagcgcacag
      421 ggtgagcagt gggaataccg ccacgacgcc agcggcagac gaacagagaa acgctgcgac
      481 cggaagaaaa tccgcttcac gtacctgtgg gacggcgaca gtattgcgga aatccgggaa
      541 taccgcgatg atgaactgta cagcgtacgg cacctggtgt ttaacggctt tgagctgata
      601 agccagcagt tcagccgggt acggcagccg cacccgtccg tggctccgca gtgggtgacg
      661 cggacgaatc atgcggtgag cgacctgacg ggccgcccgc tgatgctctt taacagtgaa
      721 ggtaaaaccg tctggcgacc gggacagacc agcctgtggg ggctggcact cagtctgccc
      781 gcagacaccg gctacccgga cccgcgcggg gaactggacc cggaagccga ccccggcctg
      841 ctgtatgcag gacagtggca ggatacggaa tccgggctgt gctataaccg gttccggtat
      901 tacgagccgg aaaccggaat gtacctggtg agtgatccgc tggggttgct ggggggtaag
      961 cagacttacc ggtatgtgcc gaatccactg gggtatattg atccgctggg gctggcaaaa
     1021 acttctgttc ccgcagaaaa aataagcctt tcggataaag caagagatct ttttagacaa
     1081 ggaaaagtaa gagaagcatt agatgttcat tatgaggatc ttgtacgaag aaagcttggt
     1141 ggtatatctc aagaaattgc cgggcgtgaa tatgatgttg ttactgacaa gatcatagcg
     1201 caggtcaaga ggacttatag ctctatagat aatccaaaaa acttcttaag taagtctact
     1261 cggacacaaa ttaagaagac aattgagctg gctgaagaac aggggaaaga ggctcagttt
     1321 tggtttaaat atggagtaag ccctaaagta agagaatata tagaaagcaa aggtgggaaa
     1381 gttatattgg gtatggggaa ttaacatgtc aagatatata cttattagag gttggtttga
     1441 gtgcgacttt gaacaagtaa aaaaaataaa agaagctaat gaagagtttt tcaaacattc
     1501 tgaaaaatat ttattaccag aaaac
//
LOCUS       DAZUCE010000445         1517 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZUCE010000445 DAZUCE010000000
VERSION     DAZUCE010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.448"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>1517)
                     /gene="ehxA"
                     /locus_tag="ACHY03_005198"
     CDS             complement(<1..>1517)
                     /gene="ehxA"
                     /locus_tag="ACHY03_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFS3908284.1"
                     /translation="VGINELVKAADDLGIEIHRTERDDTAIANQFFGTAEKVVGLTER
                     GVAIFAPQLDKLLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALD
                     ELLRKQREGEDISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPR
                     LSSVGGKLQNLPNLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQV
                     LGNVGKAVSQYILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAIADKFERAKQLE
                     SYSERFKKLNYEGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPI
                     SMLVSALTGTISGILEASKQAMFEHVADKFAARINEWEKEHGKNYFENGYDARHAAFL
                     EDSLSLLADFSRQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGG
                     LLEAQPKEFTQQVFDPQKGTIDLSTGNVSSVLTFVTPTFTPGEEVRERKQSGKYE"
BASE COUNT          410 a          366 c          278 g          463 t
ORIGIN      
        1 tattcatatt taccactctg ttttctttct ctgacttctt ctcctggggt aaatgttggt
       61 gttacaaatg tcaaaacact tgatacatta cctgttgaaa ggtctatagt ccctttttga
      121 ggatcaaaaa cttgttgtgt aaactccttc ggttgagcct ctaaaagccc tccattctcc
      181 agataattaa tatatgcctt accactctga ctgcgatcag cattacgggt tatacctgca
      241 agttcaccga tcttctcatc ccaatgttgc tgggtgattg cgacagctct ttctactgca
      301 tgctgacgag aaaaatcagc aagcaaagac agagaatctt ctaaaaacgc agcatgtctt
      361 gcgtcatagc cattctcaaa ataatttttg ccatgctcct tttcccattc attgatccgg
      421 gcagcgaatt tatctgcaac gtgctcaaac atagcctgtt ttgatgcttc cagaatgcca
      481 gatatcgtac cggttaatgc actcaccagc atgcttatcg gggcccctat gagggatgca
      541 ctggaggcgg cactaactcc cgcagataca gaactcagga cagtatttat tgttgtcagg
      601 gctgcatcta tagctccggt ttctttatga aaggctgcga gtaaagcatc cccttcataa
      661 ttcaattttt taaatcgttc agaatatgat tcaagctgct tagctcgctc aaatttatct
      721 gcaatagcca ggaaagaaag aggactgata gccagcataa cagccgatgt gatcagaccc
      781 gcacttgcag ctgttgtcga taatccctgt gccattctct gagccagaat atattgcgaa
      841 acagctttac caacatttcc aagaacctga gttgtcagtt cgatacccgc tgcagctttt
      901 gttcctgtat gtgcgtcact gtttcccaga ataaagcttg ctgatacagc agaaagaatt
      961 ccggagacaa catccagccc atcccccagg gggcccaggt tcggtaaatt ttgtaatttt
     1021 ccaccaacag aacttaagcg aggtttactg gataaaaatg agccaagctg gttaagctgc
     1081 tcagaaaatg aatcaacggt actgtttata cttgatactg tatctacaag ctgattaata
     1141 agttcaatac tacttttggc aatatcattc tgacttatat cctctccttc ccgttgtttt
     1201 ctcagcaatt catcaagagc catgccagat aaagcaatcc ccgtaaaatt ctgtagtgct
     1261 gagagaactg ttccggcttt tcccagatta ttacctacat tttcagcggt tcctcctatt
     1321 ttactcccaa ctttctgata cttctgcaga agtttgtcaa gttgtggtgc gaatattgca
     1381 acaccacgtt cagttaatcc tacaactttt tctgctgtac caaaaaactg gtttgcaatc
     1441 gctgtatcat ctcgttcagt acggtgtatt tctattccaa gatcatcagc agctttgacc
     1501 aactcattaa tacccac
//
LOCUS       DAZUCE010000446         1516 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZUCE010000446 DAZUCE010000000
VERSION     DAZUCE010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.449"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1462)
                     /locus_tag="ACHY03_005199"
     CDS             complement(<1..1462)
                     /locus_tag="ACHY03_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFS3908285.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPREFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVYFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFL"
BASE COUNT          427 a          360 c          304 g          425 t
ORIGIN      
        1 gtaaaaaaag gggatcatcg ccatgattat ccagcaggat ggcaattttt tcgcacatat
       61 tttctttcct cagctcttcc ctgggttgca gccttttcat ggtatgatgt tcaatttctt
      121 tcaggagtaa tgaggcattt atctgacgtc tggtactgcc ttgcgtcagc cagtggatgg
      181 cttgggaaaa acactgttgt gcaacggtga aataaacatc attcttgatg ctactattca
      241 gtgcctgctt ttgattatgc ctatgatgga ttagcgtcac aaccaacttt tgcaatggtg
      301 aaccatcctg attgacaata tcttgggcat gtgatttctc gcagtattga ttgagtaatg
      361 caacaaaact gttaaccagt tctttcaata tatcaggtat ctctttttgt gttataaatt
      421 ctctaggaat acccaacagt tctagataac agtaataaca tgcttcatgg aaagttttct
      481 gaaatatctc ctcaaccttc aaacgggctg caagagtgaa gtcagggtca aacatatatt
      541 ttccgatata caagcagaga tcagacagcc cttgcataag taccatcggt tcttttgcca
      601 gttcagctaa tcttagttta tacccaaaat cagctgggga atctcccgga aacagacaac
      661 tgagtccgtt aaaatccatt aatctgccat ccattgcaat attagaagga gaagttgtca
      721 atctcaggaa acgtgttcga caaaatgcca tttgccaggc ctcacgacgt gccagttcac
      781 ataacccctc aatgcaataa atccggggat cagttcgtgc ttcagcatca atttcttccg
      841 gtggtacagg tagatatcct ggcagcttgt ggatcacaga tctaacccga caggcatcgt
      901 gaattagcag actggaatac tccggtttta cttggaagta aggtgcccgt tcaaaatgcg
      961 ccggacgaac aacaggctca cgtaccaaca gggctcttcg tgatttccta ccggagcgct
     1021 caaatgcctt ttcagtatag agatctgtaa gtaaaaccgc ccgaacccgc acagcactat
     1081 aaggtaatat ttgagccagt acttctcccc acaaagcctc atatattgca tgaacagcgc
     1141 cgagtgcacc attagaatga cgttcgtcag taccttcgcc aaccaacgga ttacttccta
     1201 ttcctttaac ctggtagtta ccatcaaacc cacaacgtgc accacctcca ttacgctgaa
     1261 taccactgcc accatagcgc tctgcatgga acactttggc agaagctgta ctgccaggat
     1321 cgataatata actgcaggca ttttgcagct cgtctggatt tctgacatcc tctgcacacc
     1381 agattaatga ggccttgatc tgcctggcag tgaaaggcac caggttttca ttcagttcag
     1441 aaagtgaaca ctgatgactc atctggcagt attctccgtt tcaattttca taaaagagtg
     1501 ttcataaaag agcaag
//
LOCUS       DAZUCE010000447         1507 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZUCE010000447 DAZUCE010000000
VERSION     DAZUCE010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.450"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            512..737
                     /gene="ykgL"
                     /locus_tag="ACHY03_005200"
                     /pseudo
     CDS             512..737
                     /gene="ykgL"
                     /locus_tag="ACHY03_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            complement(772..912)
                     /gene="ykgO"
                     /locus_tag="ACHY03_005201"
     CDS             complement(772..912)
                     /gene="ykgO"
                     /locus_tag="ACHY03_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFS3908286.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(912..1175)
                     /gene="ykgM"
                     /locus_tag="ACHY03_005202"
     CDS             complement(912..1175)
                     /gene="ykgM"
                     /locus_tag="ACHY03_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFS3908287.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          447 a          304 c          325 g          431 t
ORIGIN      
        1 gcaatataac gttcaattct tacttgcatt ttatattagc caggaacttt gccctgaggt
       61 gttaggaata gtcttaatgt gttgtgtaat ggactagtaa aaaaacaagt ctaaatgata
      121 agaaatagaa tataaaacta tcatgagtca taattaaaga tgatttgcca atcagtaagg
      181 tgttttaatg gattaacatg ccgcacaaaa tggttatggg tgttcatccc aataagtgat
      241 gaaaaattaa acaggtgtaa gtacgtcatc tgtccggtaa gccccctaag agccagatta
      301 agtgaagtta aacgaagact gccagttgtc accggaacga tggcctcttt gccagggcac
      361 tgcattcagt catctgtgcg cgttactcta actggcactc gaacgtcggc gacggggtta
      421 ccggtggatc aggccgttcc tgtggcgtaa acgtttttat gtcagtacca acagatttac
      481 cacatttatc atcttaacag tttgagtgta aatgcaggct gcgtcgcaaa gggaggaaac
      541 tgagtggcgt gttcagtcta aaagagggat gatgccagca tacagggcga agctggtcaa
      601 caagtcaaca ttaatataat ggaatattcg gagagaaacg tcaggcagtt gaccttaaat
      661 gaacaggaag atacatcccc aggaaaatta atgctggtgt aataaataca ccaacattaa
      721 tccggtctga ttattaatca gacaaatatc gttaattcgt cgaattttaa atcaacgttt
      781 tttcttacga ccctgaacgg ccttaaaacg tggattagat ttacaaatca catacagccg
      841 tccttttcgc ttcacaatct gacagtctgg atggcgttct tttgcggtac gcagagagtt
      901 aaggactttc atcatgcccc ctttttcgtg ctaacaaaac gaccaaaacg ttgggtgaac
      961 cgtgcaacat ttccttctga tgccactgtt ctcagcttcc ctgtatagaa cgggtgcgat
     1021 ttcgaagaga catcaattgt tacgtacgga tacgttacgc catccagctc aatctcacgg
     1081 tctgttttga tagtcgagcc gattttaaag tactcatcaa cactggtgtc gtggaacacc
     1141 acagtacgat actcaggatg gatattgggc ttcatcattt ttacctgtta tgttataaca
     1201 taaccataaa gatacatgct catccggatc atttcaacac ccaatccctg tgaccattag
     1261 tggtatgtgg aatttcctca acggggaact ctggcattgt taacagccat tggcgacgct
     1321 cccataaacg cctggatgag tgattgatga tagtttcgct gagagtatgg atttttcgag
     1381 aatggaggtt atctccactt tactggccga tgatttatcg cagtatgatt gattacctga
     1441 cagcgtgtat tcctctccca cggatataat aattacttac aaaaagggag aggatgcata
     1501 ttttaaa
//
LOCUS       DAZUCE010000448         1501 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZUCE010000448 DAZUCE010000000
VERSION     DAZUCE010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.451"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..938)
                     /locus_tag="ACHY03_005203"
     CDS             complement(<1..938)
                     /locus_tag="ACHY03_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS3908288.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNTFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKSR"
     gene            complement(964..>1501)
                     /gene="rfaY"
                     /locus_tag="ACHY03_005204"
     CDS             complement(964..>1501)
                     /gene="rfaY"
                     /locus_tag="ACHY03_005204"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFS3908289.1"
                     /translation="KYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAG
                     LTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHAL
                     NMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYS
                     VIYRTKLRKFIKKLKGKA"
BASE COUNT          467 a          316 c          259 g          459 t
ORIGIN      
        1 tgtctggatt tcacttgata ctgcttttca ttcgtagctg gaataaacgc cacatcattc
       61 cagcaagaag cctgataagc ctcattaaaa tacttctggc agggataatt cgtccagtcg
      121 ttccagggct tcgttacgcc aacataatga atcagcttag tatcatcggt aatggtcttt
      181 ttataatctt catcgctttt attttttaac tcataatcaa tgccataaaa gcaatcataa
      241 tcgccgctaa taaaaataca atgaccaaag aacagaatat ttaaaatatc ctgatcaagg
      301 catttatact tcgtgtgctg cgggtcactt gccagcatag caatggcttt attcataaaa
      361 tcatttttcg cccatgcttc aagctgcaga tatactacac cggcgttgaa ataattccct
      421 ggaagtccct ctatagccag acgcttcgct ggtttttcct gcataaaagg cacatcttta
      481 ataacagctg cgaacttatc cccatcaaaa ttaatatcaa gcaatccagc taatgagcct
      541 ttacagatga catcagcatc gatatataac agattttcaa tagtttcact caaatactgg
      601 aacgcaacta aacggaagaa tgttgcataa gaccactgtt tcaggctggg gaagatatca
      661 ataaaggcat tggagacttt ataaattatt acttcaacgt tagcattttt gctggtaaaa
      721 gtattaacac gttgtagata atcttcatca atgtagtcgg taaagagatg aaacttaacc
      781 gcaaggtccg ggttatgcat aacaactgat tgcatagata ctgccgcacc aaataaaaag
      841 ttacggtcaa taccgtaagc gatatttaac acacgatcgg tgttagcaat ttccccagag
      901 acagcgactt cagtaatact ctcagctaat aaatccatgt tggttccgtt ttgactgtgt
      961 ggtttacgct ttgcctttta attttttaat aaatttgcgt agtttggttc tgtagattac
     1021 tgaatagtag ccataatctt ttatttcgtt ggcaataccc aggtgacgtt ccatggccag
     1081 acgatcacga gctttacgct ctgccgtaca gcttttaccg gataggtcaa ttatcctgac
     1141 accatcttta ctaacgataa agttaccccg gtgtgggtcg cccgatagca tatttaaggc
     1201 atgcaatttt tccattgaag ccttaatttc tgctttaaca ttttctggaa taatcggcat
     1261 atcattgagc tctacgccct cgacatactc gataagcatg ataaaaacac tggcatagtt
     1321 gaaaatttta cgctcagcca gaaaataaaa atcattagga aaagtaagac ctgcacttct
     1381 gacacgatcg gtttcaacga tcaaattctg atagtagtca ccttttacaa aagattttaa
     1441 aaaacgttca ttccttttcg ttttcggagc aaagacttta aaaacatagc gaccatattt
     1501 c
//
LOCUS       DAZUCE010000449         1498 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZUCE010000449 DAZUCE010000000
VERSION     DAZUCE010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.452"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..118
                     /locus_tag="ACHY03_005205"
     CDS             <1..118
                     /locus_tag="ACHY03_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFS3908290.1"
                     /translation="NANRIIVMDNGFISEDGTHKELISKKDSLYAYLYQLQA"
     gene            122..>1498
                     /gene="ehxD"
                     /locus_tag="ACHY03_005206"
     CDS             122..>1498
                     /gene="ehxD"
                     /locus_tag="ACHY03_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFS3908291.1"
                     /translation="MRFYMKGLWDLICRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVIWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGAFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNARGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRS"
BASE COUNT          529 a          221 c          317 g          431 t
ORIGIN      
        1 aaatgcaaac agaataattg ttatggataa tggatttatt tctgaagatg gcacacataa
       61 agagcttatc tccaaaaaag acagtttata tgcatattta tatcagttgc aggcataacg
      121 tatgcgcttt tacatgaaag gtttatggga tttgatttgc cgttataaga cggttttttc
      181 tgatgtctgg aaaattcgtc acacacttga tgctcctgtg agagaaaaag atgagtatgc
      241 atttcttcct gctcaccttg aactcattga aacaccagta tccagacgtt ctcattttgt
      301 tatatggagt attttattat ttgtaattat atctcttctc ttatctgttc ttgggaaagt
      361 tgaagtggtc tcagtagcaa atggtaagtt tactcatagt ggaagaagca aagaaataaa
      421 accgatagag aatgcgattg tagaaaaaat aatggtgaaa gatggcgctt ttgtaaaaaa
      481 aaatgatcca ttagttgaat tgacggtgcc tggtgttgaa tctgatattt taaaatcaga
      541 agcatctttg ttgtatgaaa aaacagaaca atatcgctac gcaatccttt ctgaatcaat
      601 acaacggaat gagcttcctg aaataaggat aacagatttt cctggcggag aagataatgc
      661 aagaggtgaa cattttcaga gggttagctc attaataaaa gagcaattca tgacatggca
      721 gaacagaaaa aatcaaaagc agctcacatt aaataaaaaa atagttgaac gggatgcagc
      781 gcttgctcgt gttagcctgt atgagcatca ggtatcacag gaaggaagaa aacttaatga
      841 ttttaagtat ttgttgaata aaaaagctgt ttctcaacat tcagttatgg agcaggaaaa
      901 tagctatatt caggcaaaaa atgaacatgc agtctggctt gcacaggttt ctcaacttga
      961 aaaagaaata gaacttgtgc gggaagaact ggcactggag acgaatatct tcagaagtga
     1021 aattatcgag aagcacagaa aatcaacaga taacattgtg ttactggagc atgagcttga
     1081 aaaaaacagg cagagaaaag catcatcttt tattaaagct cctgtgagtg gtactgttca
     1141 ggagttaaat atacatacag aaggtggtgt tgtcacaaca gcagaaacgc tgatgattat
     1201 tgtccctgat aatgatattc tcgaagtaac agcatctgta cttaacaagg atatcggttt
     1261 tatacaacct ggacaggagg tcgttattaa agtagatgca tatccatata cacgtcatgg
     1321 ttaccttaca gggaaagtaa aaaatataac tgcagattct gtttctgttc cggatacagg
     1381 acttgtattt aacgtgatta tatctgttga tcggaatgat atacagggag aaagaaaaaa
     1441 aattcctgtt acagctggaa tgactgttat ggcagaaata aaaacaggag tgcgtagt
//
LOCUS       DAZUCE010000450         1488 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZUCE010000450 DAZUCE010000000
VERSION     DAZUCE010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.453"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            198..908
                     /locus_tag="ACHY03_005207"
     CDS             198..908
                     /locus_tag="ACHY03_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS3908292.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            950..>1488
                     /locus_tag="ACHY03_005208"
     CDS             950..>1488
                     /locus_tag="ACHY03_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFS3908293.1"
                     /translation="MTAFRAAFKAYRLHQVLLLPRFVRLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPNNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDL"
BASE COUNT          424 a          348 c          299 g          417 t
ORIGIN      
        1 acaacagtag ttaaacttac tgttatttaa aatatttact ttgtagcaag gttttcctat
       61 taaaagagaa ccaacattta ttacaagcgc attaagtctg gaaacccggc gactcgccaa
      121 tgtattagca acatttaccg attgtctgat gtaacggttt tcccgcttat cctcttaaca
      181 catggagaat gcatcagatg caaactacgc gtacccccta ctctatttcc tttatggcga
      241 cagtgttact tttattgctc tttgcttgcc atagcaccgt tgccaacgca gctgtcgcac
      301 tgggtgcgac tcgggtaatt tatccggcaa accagaaaca ggttttattg cctgtcacga
      361 ataatgatcc tgccagtgtc tatctcattc agtcatggat agaaaatgca ggtgaccaaa
      421 aagacaccca gtttgtcatt accccaccgc ttttttccat gcaaggtaaa aaagagaata
      481 cgctgcggat aatcaacgcc actaatcatc agttaccagg cgaccgtgaa agcctgttct
      541 gggtgaatgt taaagctatt cctgctatgg aaaaagacca gaagaatgag aatacattac
      601 agctggcaat tattagccgc ataaaaatgt tttatcgccc cacgaacctg gcaatggcac
      661 ctgaagaagc gcctgcaatg cttcggtttc gtcgcagcgg tagcaaactc actcttatta
      721 atccaacgcc ctacttcatt accgtaacaa atatgaaagc aggaaatagc aatctcccaa
      781 acaccatggt gccgccaaaa ggtgaagtat cggtggatat cccgcatgct gcaaccgggg
      841 atatcagttt tcagacgatc aatgattacg gcgcattaac tccgcgtatt aaggcaacca
      901 tgcagtaagc cagtgaccta attaataata aactcaatcg gaattaataa tgacagcctt
      961 tcgtgccgca tttaaggcat atcgtttgca tcaggtgtta ctactgcctc gctttgtacg
     1021 gcttaccatt gcccttggct tagcaactgc ggtatttccc gttgacgcag agtattattt
     1081 taatccacgg tttttaagca atgatcttgc tgaatctgtc gatttatccg cctttactaa
     1141 gggacgcgag gcaccacctg gtacatatcg ggtcgacatt tatttgaacg acgaatttat
     1201 gactagtcgg gatattactt ttattgcaga tgataataat gcagatctca tcccatgcct
     1261 gagtacagat cttcttgtca gtcttggcat aaaaaaatct gcattattag acaataagga
     1321 acattcggca gaaaaacatg tgccgaacaa cagcgcatgc acaccacttc aggatcgttt
     1381 ggccgacgca tcaactgagt ttgatgttgg tcagcaacat ttgtcactct ccgttccgca
     1441 aatttatgtt ggcagaatgg ctcgcggcta tgtttctcct gatctgtg
//
LOCUS       DAZUCE010000451         1487 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZUCE010000451 DAZUCE010000000
VERSION     DAZUCE010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.454"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            83..325
                     /locus_tag="ACHY03_005209"
                     /pseudo
     CDS             83..325
                     /locus_tag="ACHY03_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            399..1271
                     /locus_tag="ACHY03_005210"
     CDS             399..1271
                     /locus_tag="ACHY03_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFS3908294.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSNRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMWVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVEWHRKEVFRG"
     gene            1316..1487
                     /locus_tag="ACHY03_005211"
                     /pseudo
     CDS             1316..1487
                     /locus_tag="ACHY03_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          417 a          320 c          367 g          383 t
ORIGIN      
        1 tcgcctcata cttaaaactt ccctcatacg ggtgaaggtt aaagctttgt agataggaga
       61 cagtcacaat gaataaaaca ataaattgat gtcgcacgcg ccgatgggca tggcgggtaa
      121 tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc agaacatctt
      181 caatggctat tttgaagatc tcaacgaggc tggagaatat ttagcagcta tagacaagat
      241 caatgcctat tatttcgaca aaaaagcaga agattatttc ttcaataagt ttttgttgtc
      301 ttttgatccc tcaacacagc aatggatctt cacttgcagg tttgaacagc agaaaatttg
      361 cgagcatgat tgcaattatt tcactgcctg ggatatcaat ggcttccagt aaatcgctac
      421 agcaggcaat tgccaacata aaaatttggc acaagggtga acagcgcgcg ccgcataaac
      481 cattgttact gctatacgta ttagcgggat acctgaatgg gcatccgcgc cttttcgatt
      541 atggatcgga aatctacgaa cctctgcaca gtttactgga acgttttggg ccacagcgtt
      601 cacagtaccg acctgatatg ccgttctggc gattacaagg cgacggattc tggcaattac
      661 acaatgccga gctttgctca acagcaggga gcaaccgaca accgccagtg aaggaactga
      721 acgaatacca cgttgcaggc ggtttcgatg aacagcacta cgccctggta accggcaata
      781 aaaaactcat caatactctg gcgcaacaga tactcgaagc gcacttcacc gagagtattc
      841 aggaagaaat tgctgacgag ctgggttttg atctccagca aatccgtaaa cagcgcgacc
      901 cgttatttcg caaaaatgta ttacgcgctt ataactacca gtgcgcgata tgtggcttta
      961 atatgcgcca cgacgacacc accgtcgcgc tggaagcggc gcatataaaa tggaaacagc
     1021 acggcggtcc ttgtgaaatt cctaatggcc tggcgctttg cgcaatacac cataaagcct
     1081 ttgataaagg atcgattggc ctggatgaaa atatgtgggt actggtctcg gatgcggtaa
     1141 acggcggagg gatcgttgag agattattct gggattttga cgggaaaact attgcgttgc
     1201 cgcaggtgcg taaaaattac ccttatgagg ggtttgtgga gtggcatcgg aaagaggttt
     1261 ttagggggtg aggttgataa ctatcgcagt atgttcaata ttgctgacat aatcagtgca
     1321 ggatcttgcc gctagtttac aactcgcctt cgatagcggc gcaaaacgag ttctgttgcc
     1381 gatatcctcg gctatggata ttccaacagt tccggcagag ttgtttacca agtttcaggt
     1441 aagtttttac tcagaaccgg ttgatgcggt ttataaggcg ctggatg
//
LOCUS       DAZUCE010000452         1486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZUCE010000452 DAZUCE010000000
VERSION     DAZUCE010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.455"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>1486
                     /locus_tag="ACHY03_005212"
     CDS             <1..>1486
                     /locus_tag="ACHY03_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFS3908295.1"
                     /translation="ETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDIRL
                     QLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKATQPDGHTLARLWASL
                     PPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGRTL
                     TYRREAAGDLAGGITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDRSRPLSASAF
                     PDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLVRYTYTEAGELLAVYDRSNTQ
                     VRAFTYDAQHPGRMVGHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRITVT
                     DSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNV
                     VSGDITDITTPDGRETKFYYNDGNQLTAVVYPDGLESRRAYDERDRLVSETSRSGDVI
                     RYAYDNPHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTR"
BASE COUNT          275 a          447 c          527 g          237 t
ORIGIN      
        1 gcgagacgga ccttgcgctg cccggcccgc tgccgttcat tctctcccgc acctacagca
       61 gctaccggac ccggacgcct gcaccggtgg gcgttttcgg ccccggctgg aaagcgcctt
      121 ctgatatccg cttacagcta cgtgatgacg gactgatact caacgacaac ggcgggcgga
      181 gcattcactt tgagccgctg ctgccggggg aggcggtgta cagccgcagc gagtcaatgt
      241 ggctggtgcg cggtggtaag gcaacgcagc cggacggcca tacgctggca cggctgtggg
      301 cgtcgctgcc gccggatatc cggttaagcc cgcatcttta cctggcgacc aacagcgcac
      361 aggggccgtg gtggatactg gggtggtctg agcgggtgcc gggtgctgag gacgtactgc
      421 cagcgcctct gccgccgtac cgggtgctta ccgggatggc ggaccgcttc gggcggacgc
      481 tgacgtaccg gcgtgaggcc gccggtgacc tggccggggg aatcaccggc gtgacggacg
      541 gtgccgggcg ggagttccgt ctggtgctga ccacgcaggc gcagcgggcg gaagaggccc
      601 gcacctcttc gctatcttct tctgaccgtt cccgccctct ctcagcctca gcgttccccg
      661 acacactgcc cggtaccgaa tacggccccg acaggggcat ccgcctttcg gcggtgtggc
      721 tgatgcacga cccggcatac ccggagagcc tgcccggtgc gccactggtg cggtacacgt
      781 atacggaagc cggtgaactg ctggcggtat atgaccgcag caatacgcag gtgcgcgctt
      841 tcacgtatga cgcgcagcac ccgggccgga tggtggggca ccgttatgcg ggaaggccgg
      901 agatgcgcta ccgctacgac gataccgggc gggtggtgga gcagcttaac ccggcaggcc
      961 tgagttaccg ctaccagtat gagcaggacc gcatcaccgt cacggacagc ctgaaccggc
     1021 gtgaggtgct gcatacagaa ggcggggccg ggctgaagcg ggtggtgaaa aaagaactgg
     1081 cggacggcag cgtcacgcac agcgggtatg acgcggcagg aaggctcacg gcgcagacgg
     1141 acgcggcggg acggcggaca gagtacggtc tgaatgtggt atccggcgat atcacggata
     1201 tcaccacgcc ggacgggcgg gagacgaaat tttactataa cgacgggaac cagttaacgg
     1261 cagtggtgta cccggacggg ctggagagcc gccgggcgta tgatgagcgg gacaggctgg
     1321 tatcggagac atcgcgcagt ggggacgtca tccggtatgc ttatgataat ccgcacagtg
     1381 aattaccggc cacgacaaca gatgcgacgg gcagcacccg gcagatgacg tggagccgct
     1441 acgggcagtt gctggcgttc accgactgct cgggctacca gacccg
//
LOCUS       DAZUCE010000453         1485 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZUCE010000453 DAZUCE010000000
VERSION     DAZUCE010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.456"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(16..1347)
                     /gene="rclA"
                     /locus_tag="ACHY03_005213"
     CDS             complement(16..1347)
                     /gene="rclA"
                     /locus_tag="ACHY03_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFS3908296.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPLIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAI
                     VVDKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSV
                     FMTPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRIL
                     GASLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          399 a          360 c          298 g          428 t
ORIGIN      
        1 cgtctgattt tgagtttatt tgactaatga aaatagatca ttgagtgatt cgctcatcga
       61 cggatgagta aatatctgat cgcgtaatat gctataaggc agcccggcat ccatcaccat
      121 tttcactata ttaatcatct cgtgggagtc aacacacagc agtgatgccc ctaatatacg
      181 ttgggtttta ttatcaacaa tcgcttttaa taccccacgg gtatcgttca tcactctggc
      241 gcgcggaatt gcagctacag gcaatgtcac cacctgaata tcagcaccac tctctctggc
      301 ttgttcttct gtcataccaa ccctggacag gggcggtgtc ataaatacgg aataaggcac
      361 atttttccga tcatcagtac tacgtttgcc ttcacccagt aactcatcac gtacaatgcg
      421 gtaatcatcc agtgatatgt aagtaaattg cagtccgccg gtaacatctc ccatcgccca
      481 aatattgtct gcggtggtat gtaatcgctt gtcaacgaca attgccccgc gctcgtttac
      541 tgcgataccg gcattttctg gatgtaacga agcggtagcc ggttgacgac cggaagctat
      601 taacagtgca tccaccgcca gttgggcgtg ctcgctatgc acttgcactt gcacttgatt
      661 ttcatggtga ctgattcgct ccacatgggc attgaggata atatcgacgc cctgatcgcg
      721 taaaatcgtc gcgatattat cagcaatatc ccgatcttcc cgaggcaaaa acagcgaagc
      781 tgcttctaaa atggttacct tgctgccaaa attagcgaac atagaggcga actcaacgcc
      841 aatatatccg ccgcccaaaa tacctaaatg cccaggcaat tcttttagat taagtaatcc
      901 ggtgctgtca tatactcctg gcgtggtggt aattccagga attagcggaa ccacggtttg
      961 tgcaccggta ttaataaaaa ttttctcgcc atgaatttcc agatttccct caggccgatg
     1021 aacacgcagg ctatgattat tgataaactc cgcctggccg tcgatcacgt cgatattggg
     1081 catatccgca agattatgaa aattcttatt acgtaaaaaa ttaaccactt catttttacg
     1141 ctgtatggca cggacaaaat ctgtgtgctg ctgtgcgtca tgaaccaatg ttttggttgg
     1201 gatgcagccg atattaatac aggtcccgcc atacattgca tttgattgtt cgatgagagc
     1261 cactcgccaa cctgcttttg ccagcgtgac ggctaatgtt tttccagcct tgccaaaacc
     1321 aataatcact gcctgatatt tattcatgat gatttcctgt cgaaacgcgt tcatttgaca
     1381 tcatgactat agacgcaaaa gagcaaataa catttcttca caacgcttga tattttgtct
     1441 tatcgtctgg tattttcttt ttcttaatca gaatcacaaa acaaa
//
LOCUS       DAZUCE010000454         1483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZUCE010000454 DAZUCE010000000
VERSION     DAZUCE010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.457"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(20..1363)
                     /locus_tag="ACHY03_005214"
     CDS             complement(20..1363)
                     /locus_tag="ACHY03_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFS3908297.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSPIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGIGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
BASE COUNT          481 a          356 c          297 g          349 t
ORIGIN      
        1 ttaaaccctt aatatttaat taacccgcgt aaggaccaag atggaatgtt gcagcgataa
       61 atttcagaac ttcacaaata gcaggccata cggttgccca gtcaaatatt cctgtccagc
      121 cgatgccatt ttcagcaagc ccggttaacg gaatagccca caccgtaccg aaggtctgaa
      181 taatacccag caagaacgta cagataatta cggaacggta tccgcccatg cggtttgcta
      241 atacgccaat tggaccgcca gagaaaaaca gaggtacaaa gccaggcaat accataattg
      301 ggctgtgaac cagtaataag atccccatcg aaaaaatggt gccaaaagta cagaagacga
      361 agcctaacgt tgcggcattg ggtgaaaaag gtaacagcgc tgcaacatcg acagcaggaa
      421 tagcgttagg aataaagcga tcttgccagc ctttaaacga accgttaatt tcgcccacca
      481 tcatgcgcac accctgcagc agaatgacca tatacatgga gaactgtaag ccaaggttga
      541 tgatatagat gtaccagggc ttgcctttcg ccattaactg gacattatcg atacccgttg
      601 atagcaagaa accaccgaca aacagcgtca tcacaatggc gatagccgtt acgttatggt
      661 taaaaatcgc cagccacccg ggaagtttca ggttttcagc atcgtgcttt tcagggtcac
      721 caaatttatg ggcgaatttg gagaaaaacc acaatcccaa catctggtta tgagcaatgg
      781 taaaaccggc atctttggta actttggcaa tgggcttaac aatgagcgtg gtggagaacg
      841 cccagaatac acccgttaat attccggcaa taataatgga tgtagtccag ctaaaaccca
      901 gccaaaagac aatcaaccat aataccgcct gagaatgtga aacaccggta ttccctgtca
      961 aaaatatccc tttggcacca gtataccgac caaataatac cagcagcagg ttggtaaaaa
     1021 acgccagtac aaatacatac ccgacataac caaaattatc ccccaatgca atttgcgttg
     1081 ccgctttcat tgcgtaggaa tccattaaat atccgtgaac accataggct ttacttacgg
     1141 cttctgctac cggtttaaat gttgcggaaa actggaaacc accaaataac accatcatta
     1201 atcccaccat ggcgctgacc atgccggtaa taatttttat tggcgatttc ttatcaaggg
     1261 cataaccaat acccaccagc aaaccaatta aaattgccgg ttcagttaaa aacgtttgaa
     1321 taacatctat gaatttggca aacatcgtcg gaacgccctc cataagcgta catttccgtt
     1381 gtcaatgcat gaaccttctg ctgacgttaa taatgtctca ggaaattttg tgtttcgcta
     1441 agcaatgcct ctgtatgtat tcggtaagtg cactgtagta atc
//
LOCUS       DAZUCE010000455         1483 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZUCE010000455 DAZUCE010000000
VERSION     DAZUCE010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.458"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            29..400
                     /locus_tag="ACHY03_005215"
     CDS             29..400
                     /locus_tag="ACHY03_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908298.1"
                     /translation="METKEIRRLRLKEWFKDRTLPPKEKSYLSQLMSGKATFGEKAAR
                     RIEQTYGMPGGYLDIEPEDITESPSSRSIALTPNQLELLQIFSAFPDNEQQEIIKELK
                     NKKEAMEDLVARWLARQGRRA"
     gene            420..881
                     /locus_tag="ACHY03_005216"
     CDS             420..881
                     /locus_tag="ACHY03_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908299.1"
                     /translation="MGVLEISALRIKMLLLAKGWSQAELARRIGIAQQSVQRWVCGIS
                     SPTAANLDKLSEVTGLPQYWFFLPLNEEKKDRAQDILKITPNQKELLQTFEAFPEEDQ
                     QQMLKDMKEKKEAMDRIVARWLAAQQKVNQAEYQHLQNEVHHEHSPFTDGF"
     gene            850..1041
                     /locus_tag="ACHY03_005217"
     CDS             850..1041
                     /locus_tag="ACHY03_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFS3908300.1"
                     /translation="MNTALSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VERRMAERFAKMRKERSEQ"
     gene            1042..1329
                     /locus_tag="ACHY03_005218"
     CDS             1042..1329
                     /locus_tag="ACHY03_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3908301.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWKRLRTCVQPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTISGIEIVNVVHARRQFPVTP"
BASE COUNT          485 a          312 c          325 g          361 t
ORIGIN      
        1 ttttgcgatt actaaagata atgtatgtat ggaaacaaaa gagattcggc gtttacgcct
       61 caaagagtgg ttcaaagaca ggactttgcc gcctaaagag aaaagttact tatctcagtt
      121 aatgagcggt aaagctacgt ttggcgaaaa ggcagctaga cgtattgaac aaacatatgg
      181 gatgcctggg ggatatcttg atattgaacc agaagacata acagagtctc catcatcaag
      241 atctatagca ttaaccccta accaattaga attgctacaa atatttagtg ctttccctga
      301 taatgagcag caggagatca taaaagaact aaaaaacaaa aaagaggcta tggaagatct
      361 tgtcgcacgt tggttggcac gacaaggtcg ccgagcctga cgggatatat agatatcaga
      421 tgggcgtact cgaaattagt gctcttagaa ttaagatgct tctgttggca aaaggctgga
      481 gccaagcaga acttgcgcgc agaattggta ttgctcaaca atccgtgcaa cggtgggttt
      541 gtggtatctc aagccctacg gcagctaatc tagataaact atctgaagta acgggacttc
      601 ctcagtattg gtttttctta ccattaaatg aagaaaaaaa agacagggca caggatatat
      661 taaaaataac ccctaatcaa aaggaactac ttcaaacatt tgaggcattt ccagaggaag
      721 atcaacagca aatgcttaaa gatatgaagg agaaaaaaga agcaatggat cgtatagttg
      781 caaggtggtt agcagcacaa caaaaagtca accaagcaga ataccagcac ttacaaaacg
      841 aggttcacca tgaacacagc cctttcaccg atggtttctg aatttgaaac tgtcgagcaa
      901 gaaaacagtt acaacgaatg gctgcgtgcg aaagtagcag cgagccttgc agatccgcgc
      961 ccagcaattc cccatgacga ggttgagcgt agaatggcag aacgctttgc taaaatgcgc
     1021 aaggaacgga gtgagcagta aatgttaccc gtgttatggc ttgaaagcgc agataccgac
     1081 ttggatgata taaccagcta tattgcacgc tttgacatag atgcggcaga acgtttatgg
     1141 aaacgcttaa ggacttgcgt tcagccatta tctgagcatc cgtatttata cccgccaagt
     1201 gacagagtgc caggtttgcg tgaaattgta gctcacccca attacattat tctgtaccga
     1261 gtgacaattt caggcattga aattgttaac gtagttcacg cccgccgaca gttccctgta
     1321 acaccttaat ccccctaaag caaaaccttc cccctctcaa gagggagggt tattcctgcc
     1381 catcagttaa gttaaatatt gactgtttag tttatttttg tataaactaa tttcaccaac
     1441 ccaccctgcc ccacagaacg ccaggcaata cttcgagtta ccc
//
LOCUS       DAZUCE010000456         1469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZUCE010000456 DAZUCE010000000
VERSION     DAZUCE010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.459"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            56..1309
                     /gene="ynfM"
                     /locus_tag="ACHY03_005219"
     CDS             56..1309
                     /gene="ynfM"
                     /locus_tag="ACHY03_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFS3908302.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(1358..1462)
                     /locus_tag="ACHY03_005220"
     CDS             complement(1358..1462)
                     /locus_tag="ACHY03_005220"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFS3908303.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
BASE COUNT          288 a          380 c          375 g          426 t
ORIGIN      
        1 ttttcctaac ctatgtataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag
       61 ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca
      121 gccaaatcaa tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt
      181 ctctgccgga ctggcaacat ttgcacttct ctattgtgtg cagcctatcc ttccggtgct
      241 ttcgcaggag tttggcttaa cgcccgcgaa cagtagtatt tcactgtcca tttccacggc
      301 gatgttggct attggtttgc tgtttactgg ccccctatcc gatgccattg gtcgcaaacc
      361 agtgatggta acggcgctac tattggcctc catttgtacg ttactttcga caatgatgac
      421 cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc
      481 agctgttggc atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat
      541 ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagtggtgt
      601 cttcacggac tttttcaact ggcgaattgc tctggcggca atcggttgtt tcgcgctagc
      661 ctcggcgttg atgttctgga aaatcctccc ggaatcacgc cattttcgcc cgacttcgct
      721 gcgacctaag tcgttgttta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt
      781 attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg
      841 ctatcggttg atgctctccc cctggcatgt cagtcaggcc gtggttggct tattatcgct
      901 ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca cccgctatgg
      961 gcgtggtcca gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt
     1021 attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagcag gattcttcgc
     1081 agcccactca gttgccagca gctggatcgg cccccgcgca aaacgcgcta aaggccaggc
     1141 ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg
     1201 tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt
     1261 cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagtcc
     1321 ggactgcggt aaatacccgt ccggacttat tgcctgctca aaccaacgtt aatagccatc
     1381 ctaaaataga cgaagcgcca gccaattccc gcagcgcatc tagcgtcatc aggattataa
     1441 gtacccaaat aaacggattc attttgctg
//
LOCUS       DAZUCE010000457         1467 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZUCE010000457 DAZUCE010000000
VERSION     DAZUCE010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.460"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..680)
                     /locus_tag="ACHY03_005221"
     CDS             complement(<1..680)
                     /locus_tag="ACHY03_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS3908304.1"
                     /translation="MQNIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSALTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMA
                     VNFGGFLS"
     gene            complement(680..1009)
                     /locus_tag="ACHY03_005222"
     CDS             complement(680..1009)
                     /locus_tag="ACHY03_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3908305.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRTAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1006..>1467)
                     /locus_tag="ACHY03_005223"
     CDS             complement(1006..>1467)
                     /locus_tag="ACHY03_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS3908306.1"
                     /translation="VGRIGSAIGGAFGGGASASSGTAIEAAAANFHFATGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFN
                     QNNHVVIQNDCINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          308 a          548 c          321 g          290 t
ORIGIN      
        1 atggaaagga aaccgccaaa attgaccgcc atgccgcgca tctcacaccc gcgcatgcat
       61 ttactgcatc tgtccttccg gatatccgtg gtagggttgt cgaactcatc cgccactgcc
      121 ggaccgttat acccgcactc atcgccccgg taatcccaca tacaggtgtt cgccagcatg
      181 atgcgaccgg gaaacagcgc cccgtccgtc tccgtcggtg tcgccagcac aaacgaggcc
      241 gtcatggccg tcagcgctga catctgctcc accacccacc ggtccgtcag ctcctgctcc
      301 gggtctgcct caggattgcc tgccacaaaa ttcaccgcat ccagaaaacg cgcatacacc
      361 cggcgacgga ccaccgtggc acccaccagg ctctgcaaat cctccgccat cccggtgacc
      421 agcccgaaaa gattggacac cgtcagcgac gggcgggcac tgctgccctt tccgttcatc
      481 tcaaagccgc tgccctcaat cgggtacgcc tgatattcac gcccctgcca ggtcaccggc
      541 tccccttttt cattcagctc attgcagaaa aaataccgct caccgccctg caccgtcagg
      601 tcgatttccc agagcaccac ccgcggtgac tgctctgatt taaccgactc gttcaggctt
      661 gcttcgtgaa tattctgcat cagttcacca cctgcttaaa ctccgcgctg aactcaacgc
      721 gcaacatccc gacccgcgca gaccacccgg cacaggtcac ctttatctgc cggtatgcat
      781 agggtggctt ccacaaaaat gccttccagc ccccgtgctc tgccagaaac gcttccagat
      841 gccgggcctc ctcccgggtc acggaaagca tcaccctgta cgtcttcagg tcagcattca
      901 gccctgccgc cgttcgctgc gaatacccgt caccaaaacg cacttcacgc accgacggct
      961 gcgagttcac ctccatatcc ggcttcactt tccagcgaaa ggttttcatc gcccgctccc
     1021 tgataacata ccgccatcac gcaactgcag ccggagttca tcctgcgccc ccttacgggc
     1081 catgtcatac accgccttca tcagctgcgg ccccgcctgt ccgttgatac agtcgttctg
     1141 aatcaccacg tgattgttct gattaaaatt aataccttcc gcccgccgca tctgcgccgg
     1201 acttccggca ccacccacat aaccaccttc cgcatagccg cgcatcagac ggtaaagatt
     1261 ccccacacct atccggctgg ttgcttcttt cgtgaaaaca aactccccgc ggtgaactat
     1321 ccccgcaggc tcatatttgc cgcccgtccc cgtaaatcct ccggtagcga aatggaagtt
     1381 cgccgccgca gcctcaatgg ccgtccccga ggaagcagat gcaccaccac cgaaagcacc
     1441 gccaatggcg ctgccgatac gcccgac
//
LOCUS       DAZUCE010000458         1437 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZUCE010000458 DAZUCE010000000
VERSION     DAZUCE010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.461"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(894..1073)
                     /locus_tag="ACHY03_005224"
                     /pseudo
     CDS             complement(894..1073)
                     /locus_tag="ACHY03_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1059..1217)
                     /locus_tag="ACHY03_005225"
                     /pseudo
     CDS             complement(1059..1217)
                     /locus_tag="ACHY03_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1229..1381)
                     /locus_tag="ACHY03_005226"
     CDS             complement(1229..1381)
                     /locus_tag="ACHY03_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFS3908307.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTD"
BASE COUNT          363 a          335 c          305 g          434 t
ORIGIN      
        1 aataacgttt ccattatcgc gtcctcaaca atgaattttg tgatgtggtg cctggtgcct
       61 ccaggtgacg ttaaccagtt aacaattaac gccggaatac agaatcaccc ataacgctgt
      121 ttttagcttt aactgttccg cgtgcgctta gccgcattca ccgcatcaca aaatacactt
      181 taaaaatggc ggatatccat ttccaccgaa tcaccagaaa agtgataaca gaggttgttg
      241 tggcggtgtt gtcactcagg cgtatggtca acctgacaac ccggtgcatt ttctggagca
      301 atggaggaag ccccagccat acttaccgcc gcgccatttc gcggattgcc acaaccggaa
      361 gcgcacgatc gaattacatt taacgacgac atatacagag agactaactt cgccgagcgc
      421 tttcgtgtta tgccctgact tttcagggat atgttctttc agtaaactgt cagtgccgga
      481 ttcttatccg tgtccggcgc acgaccacac gtgacagcgt gttggtctcc atcattaacc
      541 cggaacctct atggaggata atatgcaagt tgatattatt agccctgtaa tgatccatat
      601 gcttgcgcac atcaggctct tctggcacta atcaacgtcg gaaaaattga aaattccgct
      661 atgtgctttg aagagttgaa acaacttgtg cgcttgtatg aatatattaa aaaacaatca
      721 caagatacgg cgtaatattt aaaatacttt ctgttatatg ggcctgctat aatgctggcc
      781 catatttcta acccctctat aagaaaaaca tattattatg gcaatttctt cggtagctct
      841 tcagaatgct gagttgtttt aattatggtt tttaaataat catcgctttc atatttgagt
      901 gattctatgg caatccagat gcgggctttg gtgcctgcat ttggttccag ttgctgtaga
      961 cgttttgcat cttccagtag taaggcgata acgtgtttca gcttctcatc attcgcttga
     1021 ttcttgtttt caggcgaatt ttgtccgccg aatggccact tttcttcgta cagcccgata
     1081 aaggttcgac gcacgttacc ggatatatta tcgatggttt ctttttctac ggtactcagg
     1141 tcaagagtcg ccagttgaga acgaactata ttcgctgcca tttcctggaa tggtactggt
     1201 aaatctttaa attccatcgt caacctcatc agtcagtgtt tctggctaac cagcgacgcg
     1261 cgccagcttc ggttttaaac tttttacttt tggtatacgt catcgcggta aacgtgccgt
     1321 cctggttggg aaacacgccg cataccagag attcgttgtt gccaagattg agcgtatcca
     1381 tgttgacctc atttaccctt aacgccgggt cgcggaacta aaaacctgct gcgctgt
//
LOCUS       DAZUCE010000459         1428 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZUCE010000459 DAZUCE010000000
VERSION     DAZUCE010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.462"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..489
                     /locus_tag="ACHY03_005227"
     CDS             <1..489
                     /locus_tag="ACHY03_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFS3908308.1"
                     /translation="AYIPPHTGLPANSTDIAPPDIPAGFVAVFNSDESSWHLVEDHRG
                     KTVYDVASGDALFISELGPLPENVTWLSPEGEFQKWNGTAWVKDTEAEKLFRIREAEE
                     TKNNLMQVASEHIAPLQDAADLEIATEEEISLLEAWKKYRVLLNRVDTSTAQDIEWPA
                     LP"
     gene            complement(587..1177)
                     /locus_tag="ACHY03_005228"
     CDS             complement(587..1177)
                     /locus_tag="ACHY03_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFS3908309.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          377 a          296 c          337 g          418 t
ORIGIN      
        1 gcatatattc cgcctcatac aggtctgcca gcaaacagta ccgatattgc accgccagat
       61 attccggctg gcttcgtggc tgttttcaac agtgatgagt catcgtggca tctcgttgaa
      121 gatcatcggg gtaaaacggt ttatgacgtg gcttccggcg acgcgttatt tatttctgaa
      181 ctcggtccgt taccggaaaa tgttacctgg ttatcgccgg aaggggagtt tcagaagtgg
      241 aacggcacag cctgggtgaa ggatacggaa gcagaaaaac tgttccggat ccgggaggcg
      301 gaagaaacaa aaaacaacct gatgcaggta gccagtgagc atattgcgcc gcttcaggat
      361 gctgcagatc tggaaattgc aacggaggaa gaaatctcgt tgctggaagc atggaaaaag
      421 tatcgggtat tgctgaaccg tgttgatacg tcaactgcac aggatattga atggccagca
      481 ctgccgtagg gtaaaacata taaattctat aattagatgt atctttccat ttacggcaag
      541 gaagggggct tggaagacgt aaagcatctc acaccgagat tattttttat atgtcaggtg
      601 tctgaagttt tgctttggct cttaaaatgg tttgccgcga ggttttgaat tcccgggcaa
      661 tggcacttat acttacacct gacttaattc gttcgaatac cacctgtttc tgttcttcat
      721 ttaacacagg tggtcgacca aaacgtttcc ctgcgccgcg ggctcttact atcccggaat
      781 gagtgcgttc aagtaaaagg tctcgttcaa attcagcgac tgctgaaatt acttgcatca
      841 tcatttttcc tgttggactg gtcaggtcaa tgccacccaa tgctaagcaa tgcactctga
      901 tacctgtttc ggtcagttgt tccactgttt tcctgatatc cattgcatta caaccaaggc
      961 gatccagttt tgtcacaatc aattgatcac cacatttcag gcgagcaagc aaccggttaa
     1021 aaccaggacg ctcactggtt gctgctgagc cgctaatgtg ttcttcgatt atttgctgag
     1081 gtttgatttt aaaacctgca ctttcgattt cccggcgttg attttcggtg gtctgatcca
     1141 gcgttgatat ccgacagtaa gcaaaaattc gagacatagt gagactctat acgaaattgg
     1201 tgttcatatc ataatgcatc tcagaaaata attatgatta tttttgtgca tatttgtatg
     1261 tacacgttcg aaaataaacg aatgcgtatg caaccccgta attttggtga gacccaaaat
     1321 cgattttgtg aaaaatggct ttaactcggt ttgtttttcg agttccgggc ggactcaagg
     1381 aagaagaata gtgttgcgtg ttattttaac cagatttcaa gttgtttg
//
LOCUS       DAZUCE010000460         1402 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZUCE010000460 DAZUCE010000000
VERSION     DAZUCE010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.463"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..218
                     /locus_tag="ACHY03_005229"
     CDS             <1..218
                     /locus_tag="ACHY03_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908310.1"
                     /translation="TNPIGMNNLSQSLNIATATGDEVVSLDKHINTSATDTDQIQAFI
                     VSTWMASFQNDMYSEDNPISPYYKIEW"
     gene            302..805
                     /locus_tag="ACHY03_005230"
     CDS             302..805
                     /locus_tag="ACHY03_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFS3908311.1"
                     /translation="MRSPKVKFLTIFTLSILITKMSFASSACFNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKNNNIRSLDYGLMQINQMHIPMLKKRGIIQDERDLLDNP
                     CLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAMDNKKKRLQYAKKIYIVYTRLNELD
                     NRKALAK"
     gene            complement(823..1401)
                     /locus_tag="ACHY03_005231"
     CDS             complement(823..1401)
                     /locus_tag="ACHY03_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFS3908312.1"
                     /translation="MIGFLGHYPDLYEVVGGYKDLKKAIAECNKSAAQIFILGEFAGG
                     MMGSELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAKGCVWKTSHPAKLNRAIDS
                     ISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIADGKTNKEIANFLQLSRKT
                     VETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          478 a          263 c          224 g          437 t
ORIGIN      
        1 tgactaatcc aatcggtatg aataatcttt ctcaaagttt aaatatagcg acagcaacgg
       61 gtgatgaagt cgtttcatta gataaacaca tcaacaccag tgctactgat acagatcaaa
      121 tacaagcatt tatagtttca acatggatgg cgtcttttca aaatgatatg tattcggaag
      181 ataatcctat ctcaccttac tataaaattg aatggtagac aattgaccaa ataaatcatt
      241 agcgagatac tattataata gtatctcgtt cctttcattc aatcctcaca cataaaaata
      301 tatgcgctca ccaaaagtta aattcttaac catcttcaca ctttctatat taattacaaa
      361 aatgtctttc gccagcagcg cttgctttaa tgaagcagga acaatgttca gaatcgaacc
      421 aaatttgatt aaagctatcg ctcttgttga aagcaatcta aaaaaggata gcataggcaa
      481 gaatagagat aagaataaca atataaggag tttagattat gggttaatgc agataaacca
      541 gatgcacatt cccatgttaa aaaaacgtgg aataatacaa gatgaacgag atttgttaga
      601 taacccctgt ctgaacatta aaataggaac cgagatttta tataaccatt tttcacgttg
      661 tggagtaacg tggcaatgcc tggggactta taatgcgggt tttgcaatgg ataacaaaaa
      721 aaagagactg caatatgcca aaaaaatata cattgtatat accaggctta atgaactaga
      781 caatcgtaag gctttagcaa aatgagccta aagcctcttt ttttatatag tgcaaacacc
      841 catacgtaaa gctgttttga tcaactcgat accactgtga acatctaatt tcttcatgat
      901 attgagtcta tgagtttcga ctgttttcct gcttaactgc aagaaattag cgatttcttt
      961 gtttgtttta ccatcagcta tgagttgtaa tatttctgac tcacgatttg taagttgatt
     1021 atcagaggaa tacctggaag atattttttc acaatccata tgtacactat caaaataagt
     1081 gtagccatta ctaatcgaat caatagcgcg atttagtttc gccgggtgac tggttttcca
     1141 tacgcatcct ttcgcacctg cttcaagcaa ttttattgaa tcaatatacg gcatctttgc
     1201 tacaaatgta ataatatgag catctatttt atgcgattta acccatttta cgagttcgga
     1261 ccccatcatt ccacctgcaa attctcctaa aataaatatt tgggctgcag atttgttaca
     1321 ctccgctata gctttcttca gatctttata gcccccaaca acctcatata aatcaggata
     1381 atgtccgagg aatccaatta tc
//
LOCUS       DAZUCE010000461         1399 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZUCE010000461 DAZUCE010000000
VERSION     DAZUCE010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.464"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1323
                     /locus_tag="ACHY03_005232"
     CDS             <1..1323
                     /locus_tag="ACHY03_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HFS3908313.1"
                     /translation="IDILRQQNANIPLKEASSQSEEPHQLVNSKSEGNLHNSASKDNN
                     IGAMLKKDVEKLSTLVSERSPRNEVWKCQHDIQLNFVKNMLRNFPTENLSEKGSALLH
                     ESYLLIKNVLQLTENVNTIDRKLKDPAKIFALHNSVRKTLANVAHNTTRTSAMATTKG
                     TLTHTIASLDSFAPEMRSSATSSTLTASSSSEFKSDKYICSDIADLMSVLGSDYLEIY
                     ANSVEIMSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHFYYIDKYPNG
                     DFHYHYNPDYVLYPPAPADKIGVPLEEAEKWCAALGLPVIPPDPKHRTPSPIVEVEPQ
                     GSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAEYQAWLAGYN
                     SQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
BASE COUNT          472 a          297 c          274 g          356 t
ORIGIN      
        1 atagatattc tacgccagca gaacgcaaac attcctctta aagaggcatc ctcgcaaagt
       61 gaggagcctc atcaactggt aaacagcaaa agcgagggaa atttgcataa cagcgccagt
      121 aaggataata atattggcgc tatgctcaag aaagatgttg agaaactatc aactttagtc
      181 agtgaacgta gcccgcgaaa tgaagtctgg aaatgccagc atgacataca acttaacttc
      241 gttaaaaaca tgctcagaaa tttcccaact gagaacctgt cagagaaagg atccgcgctc
      301 ttacacgaaa gttatttgct gataaaaaac gttctgcagc ttacagaaaa tgttaatact
      361 attgacagaa aattaaagga tcccgctaaa atatttgccc tgcataactc tgtgagaaaa
      421 acgctggcta acgttgctca caacacaacc aggacaagtg caatggcaac gaccaaagga
      481 accttaactc acactatcgc ttctttagat agttttgcgc ctgaaatgag aagttcagca
      541 accagcagta ctttaacggc cagtagttca agcgaattta aaagtgataa atatatatgc
      601 agtgatattg ctgatttgat gagtgtgctc ggtagcgact acctcgaaat ctatgcaaat
      661 agcgttgaga taatgagcgc atactggcag gacttcagtg aacatatcca aagcaatatg
      721 ggaaaatgga ctcactctaa taaaaaaggc gatgcgatag tttttgatgt caatgcattt
      781 caaaaggctt tgatgcattt ttattatatc gataaatatc ctaatggtga ctttcattat
      841 cactacaatc cagactatgt tttataccct ccggcacctg ctgacaaaat tggtgttcca
      901 ctggaagaag cagaaaaatg gtgcgccgct ttaggacttc cggtcattcc acccgatccc
      961 aaacaccgca caccttctcc gattgtcgaa gttgaaccac aaggtagcgg tctgtatgtt
     1021 attattccca accctcagat tattgacagt atgagtcagt cttcagactc aatggtacac
     1081 agggatgata aagggaagga aaaaaatata tctaaagaat tcaccggcta cgaaataagc
     1141 acggctgaat accaggcatg gctggcgggc tataacagcc aggcagaaaa catgaaaacc
     1201 gacgtacagg ttatcacgac taaatacagt acagccaact ctacctacga tacgattatc
     1261 aaattattat cttcaaccat tactgcgttg tttgattcag cgaaagatta tttacgtttc
     1321 tgattaagta acacctacaa ttatcgtcca ctatctggtc ccgtaaaatg ggaccagcgt
     1381 ttcatattat taagcatca
//
LOCUS       DAZUCE010000462         1397 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZUCE010000462 DAZUCE010000000
VERSION     DAZUCE010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.465"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(68..946)
                     /gene="glxR"
                     /locus_tag="ACHY03_005233"
     CDS             complement(68..946)
                     /gene="glxR"
                     /locus_tag="ACHY03_005233"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFS3908314.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETAHQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKTLALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1046..>1397)
                     /locus_tag="ACHY03_005234"
     CDS             complement(1046..>1397)
                     /locus_tag="ACHY03_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFS3908315.1"
                     /translation="EPINHFDIPGFHLTGTRQALKLIDDVGCCNLKIQYDIYHMQRME
                     GELTNTMTQWADKIGHLQIADNPHRGEPGTGEINYDYLFKVIENSDYNGWVGCEYKPQ
                     TTTEAGLRWMDPYR"
BASE COUNT          352 a          359 c          307 g          379 t
ORIGIN      
        1 ggctgctttc ttttttaata ttgctggccg attcattttc tgatatcggc catttttatt
       61 gcgggtatca ggccagttta tggttagcca ttaattccag cgcctgcact aacgcagagt
      121 gatccaactg gctgccaccg tttgccgcac aggtattaaa taactcctgg caggtcgcgg
      181 tgtttggcag gttcagcgcc agtgtcttcg cactttgcag tgccaggttg agatctttct
      241 ggtgcagcgc aattttgaaa cctggattaa aggtgcgttt aatcatgcgc tcaccgtgaa
      301 cttccagaat acgtgaggaa gcaaagccgc ccatcagcgc ctggcgcaca cgtaccgggt
      361 ctgcaccggc ttttgaagca aacagcagag cttcagagac cgcttcaata ttgagcgcca
      421 caataatctg atttgccact ttgcaggttt gaccgtcgcc gttaccgccc acaagggtga
      481 tatttttacc gagcagttca aacagcggtt taacacgttc aaataccgct tcgtcaccgc
      541 caaccataat tgacaacgtc ccttcacgcg cgccgatttc accgccggag actggcgcat
      601 cgagataatc accgcccagt tcattcacct ggcgagcgaa acgtttagtt tcaatcgggg
      661 aaatggagct catatcaaca atggttttgc cctttagcga ggctttggta caaccatttt
      721 caccgaatag aacttcttca acctgtggtg tatccggcac cataataaaa atgatgtccg
      781 atgcttccgt tacctggtgc gcagtttcaa cactgacggc acccagtgac agtaattcat
      841 cagcaaccgg accaatggtt gtgacatgca gttgatgacc ggcacgcgcc agattaatgg
      901 ccatcggtgt acccataatg cctaagccaa taaatcccag tttcataatt aacctctttt
      961 aaattttgct tttcctggat tcagacaaca cgatcccctg ccaacttagc ggcctaaaaa
     1021 agcattgcct gaacagcgtt acgttttaac ggtacggatc catccagcgt aaaccggctt
     1081 ccgtggtggt ttggggttta tattcacaac caacccaacc gttgtagtca gaattttcga
     1141 ttaccttaaa gagataatca taattaattt ctccggtccc cggttcgccg cgatgcggat
     1201 tatcggcaat ttgcaggtga ccaattttat cggcccactg agtcatggtg ttggttaatt
     1261 caccttccat ccgctgcatg tgataaatgt catactgaat tttcaaattg cagcaaccaa
     1321 catcatcaat caatttcagc gcctgccgag ttccggtgag atggaaacca ggaatatcaa
     1381 aatggttaat aggttca
//
LOCUS       DAZUCE010000463         1379 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZUCE010000463 DAZUCE010000000
VERSION     DAZUCE010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.466"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..920)
                     /locus_tag="ACHY03_005235"
     CDS             complement(<1..920)
                     /locus_tag="ACHY03_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3908316.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVVAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSGMHIRLTEAGAIPAEE
                     WLLACDAASRLRCVSELLWLNAMLPWYNRVTDALTDICRQEHLYFRPVAVGFCLTSVA
                     ARPGNIWQQQLTSLTALPPSAGVSSQVLPLP"
     gene            complement(923..>1379)
                     /gene="tssL"
                     /locus_tag="ACHY03_005236"
     CDS             complement(923..>1379)
                     /gene="tssL"
                     /locus_tag="ACHY03_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFS3908317.1"
                     /translation="FCALLDESVLNREKTDDGWHTWQQDPLQAHFFGTLNAGEELWER
                     IREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVPAFTFAQDA
                     PVVVRAPGYRAGRTMYWLSWVAGIMTLAGLWCVLSSVLADQVARLTVQG"
BASE COUNT          335 a          424 c          364 g          256 t
ORIGIN      
        1 ggcaacggca gtacctgtga tgacacgccg gcagacggtg gcagtgcagt gagggacgtc
       61 agttgttgtt gccagatatt accgggtcgg gcagcaaccg acgtcagaca gaatccgacg
      121 gcaaccggcc ggaaataaag gtgctcctgc cggcaaatat ctgtcagggc atcagtaacc
      181 cggttgtacc agggcaacat ggcattcagc cataacagtt cactgacgca ccggagcctg
      241 gacgctgcat cacacgccag cagccattcc tctgccggaa tggctccggc ttccgtcagg
      301 cgtatgtgca ttcccgacag gtccggtgtc agtgtaaacc agtgtgcaga gtgcttattt
      361 aaggatgtgt tttgtggtga cggccctgaa accatcaatg tcaccagcac cggcggcatg
      421 gtattcagcc actgccggca ctgcatgact gcacgctgcc agttccgcaa ccacgacagg
      481 aaagcttcat ctgaggtatg ctgctccggt aaaagtccca gcatgaccac gacctggcca
      541 cacagggccg gacggctgta cgacaaccgc tctgccagta ttctcatctg ctccggcgta
      601 tccacacgaa gataccatcc cgatgctgtt tcccggtatg cctgaccggg ttcgaaccac
      661 gcatctgagt ttccggcaac aagcagcaca tgcccctgaa aatactcagg cggcagaccg
      721 tcatcgccgg cattctccgg gatatcacga cgggaagaca gacaacgcca ctgataccac
      781 agcacaagcc ccgccaccac gactgaaacc agggtcagta ttatctggtt tactgcagac
      841 agtggcagga atcccgaaat cagccacagt gtcaggaaca gactcagtat tgtcagccag
      901 agacgggaac gtacgtgcat ggttatccct gcacggtaag ccgtgccacc tggtctgcga
      961 gcacagaaga aagcacacac cagagaccag ccagcgtcat aatacccgcc acccatgaca
     1021 accagtacat cgttctgcca gcacggtacc ccggtgcacg caccaccacc ggcgcatcct
     1081 gtgcgaaagt aaatgcagga acacgcgctg tcagtgcgcg tattacatct tcgcgacgct
     1141 catcatcctg cgaccggtac tgaccggtaa aaccgagctg aagcgtacgg cagaggcatg
     1201 tcagtaccgc cacatccggc gctggtaatt tcagttgctc acgaatacgc tcccagagct
     1261 cttcaccggc attgagcgta ccaaaaaaat gagcctgcag cgggtcctgc tgccaggtgt
     1321 gccagccatc gtctgttttt tcacggttca gcacactctc gtccagcagg gcgcagaag
//
LOCUS       DAZUCE010000464         1346 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZUCE010000464 DAZUCE010000000
VERSION     DAZUCE010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.467"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            108..1160
                     /gene="yhiM"
                     /locus_tag="ACHY03_005237"
     CDS             108..1160
                     /gene="yhiM"
                     /locus_tag="ACHY03_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFS3908318.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     SSKEHWLWCYWVIFLGSITVLQGIYVLVSSDASTRLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT          303 a          298 c          310 g          435 t
ORIGIN      
        1 gttatgctat gcatttgtct ttaatgatat ctcgtcattg tattaacagg ctgaaattat
       61 cattgatgca catttcaata ttagcaggga tacctgagga gtttaaagtg aacatatata
      121 tcgggtggct tttcaaatta atccctttaa ttatgggctt gatttgtatc gcgttaggtg
      181 gctttgtgct ggaaagttca gggcaaagcg agtatttcgt cgcgggtcat gtgctcattt
      241 ctctggcagc catatgcctg gcattattca ctaccgcatt tattatcatt tcgcagctca
      301 cgcgcggcgt taatacgttt tacaatacat tgttccccat tattggctat gcggggtcaa
      361 ttatcaccat gatatggggt tgggcactgt tagcaggtaa tgatgtgatg gcagacgagt
      421 ttgtcgccgg ccatgttatt ttcggcgttg gtatgattgc cgcctgtgta tcgacggtgg
      481 cagcgtcatc cggtcacttt ctgctcattc ctaaaaacgc agcggggagc aagagcgacg
      541 gaacaccggt acaggcttat tcttcattaa tcggtaattg cctcattgcc gttcccgtgt
      601 tactcaccct gctcggtttc atttggtcta ttacgctgtt acgtagtgcc gacataactc
      661 cgcattatgt cgccggtcac gtattgctcg ggttaaccgc aatctgtgcc tgtctaattg
      721 gccttgttgc cacaatcgtc catcaaacac gtaatacgtt ctcttccaaa gagcactggc
      781 tgtggtgtta ttgggttatt tttctcggct caatcacggt gctgcagggc atatacgtgt
      841 tagtcagttc cgatgcaagc acccgactgg cccccggcat tattctcatt tgcctcggaa
      901 tgatctgtta cagcatattc tcaaaagtct ggctactggc actggtctgg agacgtacct
      961 gttcgttagc caacagaata ccgatgattc ccgtcttcac ctgcctgttt tgccttttcc
     1021 tggcatcgtt tcttgcggaa atggcgcaga ccgatatggg atattttatt ccttcgcggg
     1081 ttctggtcgg tttgggagcg gtatgcttta cgttgttctc aatcgtttca atattagaag
     1141 cgggttctgc taaaaaataa ttgcaacgta ccggataaaa ccagcgttga ccatttgcgt
     1201 aacgctggtt tttcttaggt agaatgaaat aacgcagcat taatgcatag tggttaagta
     1261 taaaaaacag caaagttact gttttttcaa cctgttcata ttttataaag atcagcccac
     1321 tgggtgagtt ttcaatcacc tttccc
//
LOCUS       DAZUCE010000465         1345 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZUCE010000465 DAZUCE010000000
VERSION     DAZUCE010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.468"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1232
                     /gene="ygeH"
                     /locus_tag="ACHY03_005238"
     CDS             <1..1232
                     /gene="ygeH"
                     /locus_tag="ACHY03_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFS3908319.1"
                     /translation="VVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIET
                     IYRKGYRFSGQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKID
                     GLYTYPMAATNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLI
                     ASNHLPVDELHNTSQFIIANILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELY
                     EFTPESIYRAMTIFDGLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALE
                     LLDYVSDITNVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNF
                     HNEKIEEARICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNMKLYYKETESESHRV
                     IIDNILKLKQLTRICMR"
BASE COUNT          491 a          233 c          224 g          397 t
ORIGIN      
        1 atgttgtact gaaagatatg atcatcgaat cagtatggaa aaatattatt gttagtgacg
       61 agtctctgac aagatgtatc tattctttgc gctgcatttt tgaaaaaatt ggctatgatc
      121 gctgcataga aacaatctac cggaaaggtt atcgtttcag cgggcaggtt ttcaaaacta
      181 aaattaatga agataatact tcagactatt ccatagctat attccctttc accacttcat
      241 tgaatacact ggatccatta atacttaatc aggaattagt acaaatcatt tcaaataaaa
      301 aaatcgatgg tctctatacc tatccgatgg ctgcgacaaa tttttgtaat gaccacatat
      361 ctcaaaattc attcttgagc agattcaagc cagattattt cgttacagga agaataaacc
      421 agaataatgc agtgaacact ttatacattg agttgatcga cgctaaaaac cttttcctca
      481 tcgccagtaa tcatctccct gttgatgaac tacataatac atcacaattt attatagcta
      541 atatccttca aacggtacat aaaccagaac gatctgtaag attagctaag caggaccaag
      601 gatataagaa tcattattta tcagatgaaa tgttagccgg aaagaaagaa ctttacgagt
      661 tcacccctga aagcatttac agggccatga ctatatttga tggattacaa aataaaagtg
      721 atatacagac gctaaaaaca gaatgttatt gccttctagc ggaatgccat atgtctttgg
      781 cacttcatgg aaaaagtgaa cttgaacttg ctgctcaaaa agcattagag cttttagatt
      841 atgtatcaga cataaccaat gtcgatggaa aaattttagc tattatggga ctgataactg
      901 gtctgtctgg acaagcaaaa gtatctcata tcttatttga acaggctaag atacactcaa
      961 ctgatatagc ctctctctac tactataggg cacttgtcaa ctttcataat gaaaaaattg
     1021 aagaggcaag gatttgtata gacaaatcac tacaactcga acccagaaga cgaaaagcag
     1081 ttgtgataaa agaatgtgta gatatgtatg tgcctaaccc gctcaaaaac aacatgaaac
     1141 tctactataa agaaactgag agtgaaagcc atcgagttat aattgacaac attttgaaac
     1201 taaagcagtt gacgagaatt tgtatgcgat aaatttttat tgacgcaagt tcattgttta
     1261 aatgtatgca acttgttatt taaacaacag agataaaaac atctaagata tttaccttat
     1321 tgcaagatat ttaaaatact ctaga
//
LOCUS       DAZUCE010000466         1335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZUCE010000466 DAZUCE010000000
VERSION     DAZUCE010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.469"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..137
                     /locus_tag="ACHY03_005239"
     CDS             <1..137
                     /locus_tag="ACHY03_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3908320.1"
                     /translation="GNKTLIDRVSYRPLQVTFDGSQWRTVSVGEYVSSGASGFEYFPL
                     "
     gene            179..844
                     /locus_tag="ACHY03_005240"
     CDS             179..844
                     /locus_tag="ACHY03_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFS3908321.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     AREMKGDEINAWRNAMEAANYTFEHNGHKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVEVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     DWK"
BASE COUNT          395 a          249 c          393 g          298 t
ORIGIN      
        1 acggaaacaa aacccttatt gacagggtat cttacaggcc gcttcaggtg acatttgatg
       61 gttctcagtg gcgtacagtc agcgtgggtg agtatgtgtc gtcaggagct tcggggtttg
      121 agtattttcc attataaagc gcatcatctt tcagacatgt aaccgggagg actgacagat
      181 gcacataaag aatttcagac aatacacgcc ggaaaatccg gatgtgccgg gtgcgatgta
      241 cctgaaatca gaggatggtc aggactggta tgagtgtcag tcgttatttt cagcagagac
      301 gctgaaggtg gtttataaca gtgccggggt cattaccggc atcagcaggg tggcatcagt
      361 tctgtggcca gtgggccaga gtgtggtgga ggtggcggat acggaagaaa accgcaaagc
      421 tgacatctcg gggcgctggg gttttgacgg ggagaaaatc acggacctgc tgaccgcgga
      481 gaaagcgcga gagatgaagg gcgatgaaat taacgcctgg cgtaatgcga tggaagcggc
      541 gaactacacg tttgagcaca atgggcataa atgggactac gggaagtcaa cgcagacgcg
      601 tcttgagccg tcggtggcgg cagcgaaagc ggggaaactg ccggaggcgt ttttctggac
      661 ggatgcggaa aacaatgatg tggaagtgac agcagaagag cttatagcgc tgagtgaagc
      721 ggcagagcag gcgatgttca ccaaagggat ggaaatccac gttcgccagc gcaccatgaa
      781 gaaggagctg gaaaagctga ccagtgcgga tgagatactg gcatacaggg tggactggaa
      841 ataaaaagaa ggggagcgga tgctcccctg atatctgagt agtatgctgt ttatcgttat
      901 cattcatcaa aagaatatta ccgtatggca cggcagcaga acatgaaatt tgtctcagaa
      961 ctgcaaatca gcattacggg tagtcaggca gataacacaa cgggataaaa aaacagcaaa
     1021 atgcattgat aaaaaatttt tacaggtagg ttaccgggcg ttatgtgacg catatcaaaa
     1081 acagaactgt aacgggagat aacagaatga tggattctgg cgtggagtaa cggcgcaatg
     1141 cgttccggga tggtgtttct gagaatgtgg ctgaaaacgc ccacaataca gtaagaaaaa
     1201 tcctaatcga ttatattaat ttttacggaa ctattcggaa tacccttatg aaaggcaacg
     1261 aagtgtcagt atttttaatg tgttctgaca cgcgttgctc tgtttgtata caaatcattt
     1321 catagggtat accag
//
LOCUS       DAZUCE010000467         1324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZUCE010000467 DAZUCE010000000
VERSION     DAZUCE010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.470"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>1324)
                     /locus_tag="ACHY03_005241"
     CDS             complement(<1..>1324)
                     /locus_tag="ACHY03_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFS3908322.1"
                     /translation="AVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLM
                     TAFFEAYLDADYTDRGLLTKEWMKGNRALRISRTPSGANAGGGILTDRGEGFVHDDAS
                     VERDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSR
                     AIVVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVT
                     KEGKQDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMNAQPQINSVDAKYQKLR
                     AELYEARKNIPPKNPVDADKLLAASHGEFVEGISDPNDPKWVKGIETRDSVNHNQQET
                     EQKGHKAEQHSPNAQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDAT
                     YQETFNEESQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETGETAN"
BASE COUNT          252 a          331 c          336 g          405 t
ORIGIN      
        1 gatttgctgt ctcgcctgtt tcattattgc cattggcatg ttgatcgcct tcggtgcact
       61 ccttgttcgg gagtccggca ctttccattt cctccggatc tttttcccgg gcttcatcct
      121 ggctttcttc gttgaatgtc tcctggtatg ttgcgtcgcc catcaccgcg ccacagtcag
      181 gacagttgcc gccgccggtc tgaccgcagg ctgtgcagac tttttccggt tcctgttgcg
      241 ctactggttc aggctgtttc gtttctggct cgttttgttg cgcatttgga ctgtgttgtt
      301 ccgctttatg gcctttctgt tccgtttctt gctggttatg gttcacagaa tcgcgggttt
      361 caatcccctt cacccatttt ggatcattcg ggtcgctaat cccttcaaca aattctccgt
      421 gagaagcagc cagtaattta tctgcatcga caggattttt gggtggaatg tttttcctgg
      481 cttcatagag ttctgcacgc agtttctgat atttcgcatc aacagaattt atctgtggct
      541 gagcattcat cgactgcgtg tcctgattat gttcagttgt atccggttcc actgcttcag
      601 tcgttgcctg ttcatctgcc attgcgccag atggctgcgg tttttcttca tcatcctgtt
      661 ttccttcttt tgttacacgc tgcggcattg gagcagagga gcgaccgcag gcaatatcca
      721 cgatttctgg atcagggttg gcatggtcgg tttcggtcag tactttgttc agatattcgg
      781 taacacggtg gggagtagcc tcgataccaa ttggtgcttc tttcacggac gcaaccacta
      841 tcgcgcggga ataatccagg tgaccaggca tggcgatgaa tttgtcacga aaaacagaaa
      901 agggcggctt attctctgaa acgatttctt caatgcgttt ggcgtgtgcc ggatgtggat
      961 tgtaaatatc gatgtccatt gaacgggcca gaacgccagc ggcaacgtca cgttccactg
     1021 acgcatcatc gtggacaaaa ccttcaccgc gatcggtaag aattcctccg ccagcattag
     1081 cgccggatgg cgtgcggctg atgcgtaaag cacgatttcc tttcatccac tcttttgtca
     1141 gcagacctcg atcggtatag tcagcgtcca ggtatgcttc gaaaaaagca gtcattaacc
     1201 ccagactgga attaacggga ttagggaaaa ctttgtctgt gtcgcgtacc agtttgtgaa
     1261 ggtcgcgaat ctccagcggg tcgagcagct ttgtgttgtg ggaaatagcc agggcagtaa
     1321 cagc
//
LOCUS       DAZUCE010000468         1312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZUCE010000468 DAZUCE010000000
VERSION     DAZUCE010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.471"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>1312
                     /locus_tag="ACHY03_005242"
     CDS             <1..>1312
                     /locus_tag="ACHY03_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFS3908323.1"
                     /translation="APLGLVAASALGAVSLVCQNLIDVCRLNTLRGPVSLFLLTLAES
                     GERKTAVDKLLMEPLYQQEMLLYSRHKNELTTWKNKEELLKAQKKALLSKLNKELRKG
                     ADESETLRQLEALQKNRGEKPVRYKFIFNDATTAAIKDQLCGQWRSVGIMSDEAGIIF
                     DGYTLSELPFINKMWDGSVLSVDRKNEPEQMIENARMTLSLMVQPGLFDRYMERKGSV
                     ARDSGFLARCLISKPATTQGKRFINGAVTPGGSLTAFHERLMELARGSIEKSSKDERY
                     CLHFSPEAQKIFIDHYNVLEQDLSPSGPLSQFRGHVSKKTENIARIAALLQYFSQGEG
                     KISADIMTSAVVISSWYTDEYKKLFALPDESELQLQDARELLDWLIEECRGECPPRVR
                     KNYILQCGPGRFRNRKKLNVLLNILASQLRLSVVQEGKTTYVLLS"
BASE COUNT          374 a          276 c          329 g          333 t
ORIGIN      
        1 ggctcccctg gggcttgtgg cagcatctgc cctgggcgct gtttcacttg tctgccagaa
       61 tctgattgat gtctgcagac tgaatacact gcgggggccg gtttctcttt tcttactgac
      121 gcttgctgag tccggggaac gaaaaaccgc agtggacaaa ttattaatgg agcccctgta
      181 ccagcaggag atgctgttat attccagaca caaaaatgaa ctgacgacat ggaaaaataa
      241 ggaagagctt ttaaaggcgc agaaaaaagc attgttgtca aaactgaata aagaactccg
      301 caaaggcgcg gatgagtcag aaacgctcag acaactggaa gcgcttcaga aaaacagagg
      361 ggaaaaacct gtaagatata aattcatttt taatgatgcc acgacagcag caattaaaga
      421 tcagctttgt ggtcagtggc gttcagtggg tatcatgtct gatgaagccg gtataatatt
      481 tgatgggtat accctgtctg agctgccttt tattaacaaa atgtgggatg gctccgtatt
      541 gtcggtggac agaaagaatg aacccgaaca gatgattgaa aatgccagaa tgacattatc
      601 cctcatggta caacccggtc tttttgaccg atatatggag cgaaaaggtt ctgttgccag
      661 ggacagtggt tttcttgccc gttgtctaat cagtaaacct gccaccacgc aggggaaacg
      721 ctttattaac ggagctgtga ccccgggagg atcattaacg gcatttcatg aacgattaat
      781 ggaactggcc agaggcagta ttgaaaaatc cagtaaggat gaacgttatt gtctgcattt
      841 ttctcctgaa gcgcaaaaaa tatttattga tcactataat gttctggagc aggatttgtc
      901 tccttccgga cccttaagcc agtttcgggg gcatgtttca aaaaaaacag aaaacattgc
      961 gagaattgct gcgttattgc agtatttcag tcagggtgaa gggaaaatct ctgccgatat
     1021 catgacgtct gctgtcgtaa taagttcgtg gtacacagat gaatataaaa aattatttgc
     1081 gttacctgat gaatctgaac tgcagctgca ggacgcccgg gagctgctcg actggctcat
     1141 tgaggagtgc cgcggagaat gcccgccccg tgtcaggaaa aattatattc tccagtgtgg
     1201 cccgggccgg ttcaggaaca ggaaaaagct gaacgtcctg ctgaacattc ttgcatctca
     1261 actcaggctc tctgtcgtgc aggaaggaaa aaccacctat gtcctgcttt cc
//
LOCUS       DAZUCE010000469         1291 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZUCE010000469 DAZUCE010000000
VERSION     DAZUCE010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.472"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            32..1144
                     /locus_tag="ACHY03_005243"
     CDS             32..1144
                     /locus_tag="ACHY03_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HFS3908324.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
BASE COUNT          323 a          314 c          352 g          302 t
ORIGIN      
        1 ggtattacgc ctgatatgat ttaacgtgcc gatgaattac tctcacgata actggtcagc
       61 aattctggcc catattggta agcccgaaga actggatact tcggcacgta atgccggggc
      121 tctaacccgc cgccgcgaaa ttcgtgatgc tgcaactctg ctacgtctgg ggctggctta
      181 cggccccggg gggatgtcat tacgtgaagt cactgcatgg gctcagctcc atgacgttgc
      241 aacattatct gacgtggctc tcctgaagcg gctgcggaat gccgccgact ggtttggcat
      301 acttgccgca caaacacttg ctgtacgcgc cgcagttacg ggttgtacaa gcggaaagag
      361 attgcgtctt gtcgatggaa cagcaatcag tgcgcccggg ggcggcagcg ctgaatggcg
      421 actacatatg ggatatgatc ctcatacctg tcagttcact gattttgagc taaccgacag
      481 cagagacgct gaacggctgg accgatttgc gcaaacggca gacgagatac gcattgctga
      541 ccggggattc ggttcgcgtc ccgaatgtat ccgctcactt gcttttggag aagctgatta
      601 tatcgtccgg gttcactggc gaggattgcg ctggttaact gcagaaggaa tgcgctttga
      661 catgatgggt tttctgcgcg ggctggattg cggtaagaac ggtgaaacca ctgtaatgat
      721 aggcaattca ggtaataaaa aagccggagc tccctttccg gcacgtctca ttgccgtatc
      781 acttcctccc gaaaaagcat taatcagtaa aacccgactg ctcagcgaga atcgtcgaaa
      841 aggacgagta gttcaggcgg aaacgctgga agcagcgggc catgtgctat tgctaacatc
      901 attaccggaa gatgaatatt cagcagagca agtggctgat tgttaccgtc tgcgatggca
      961 aattgaactg gcttttaagc ggctcaaaag tttgctgcac ctggatgctt tgcgtgcaaa
     1021 ggaacctgaa ctcgcgaaag cgtggatatt tgctaatcta ctcgccgcat ttttaattga
     1081 cgacataatc cagccatcgc tggatttccc ccccagaagt gccggatccg aaaagaagaa
     1141 ctaactcgtt gtggagaata acaaaaatgg tcatctggag cttacaggtg gccattcgtg
     1201 ggacagtatc cctgacagcc tacaaaacgc aattgaagaa cgcgaggcat cgtcttaacg
     1261 aggcaccgag gcgtcgcatt cttcagatgg t
//
LOCUS       DAZUCE010000470         1284 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZUCE010000470 DAZUCE010000000
VERSION     DAZUCE010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.473"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            267..785
                     /locus_tag="ACHY03_005244"
     CDS             267..785
                     /locus_tag="ACHY03_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFS3908325.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            995..>1284
                     /locus_tag="ACHY03_005245"
     CDS             995..>1284
                     /locus_tag="ACHY03_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFS3908326.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCP"
BASE COUNT          319 a          313 c          325 g          327 t
ORIGIN      
        1 ttcagctttg gccattttat ggaggcaata cggactttga acagttgggt atggttatcc
       61 agatatatat tattaagtta ttgctgaata agagatttaa tgattttaaa aatagttttt
      121 acgacaaccg taaattctag ctcggtttgt atgtaaaaaa tactgaaaaa tgatgcggtt
      181 ccgtgaagtg agctgcatct gcctgtctgg gattaacggc aggtcattgt aagtagtaat
      241 taaccacgtt aacaaggagt ttacaaatgc caacaccgtg ttatatctcc atcactggtc
      301 agacccaggg aaatattact gcaggtgcat ttaccgctga ttctgtcggc aacatctatg
      361 tgcaggggca cgaagacgag atgctggtgc aggaattcct gcataacgtt actgtcccga
      421 ccgatccaca gtccggtcag cctgcgggtc agcgtgctca caagcctttc atttttaccg
      481 tggcgctgaa caaagcggtt ccgctgatgt acaacgcact ggcctccggc gaaatgctgc
      541 caaccactga acttcactgg tggcgtacct ctgtggaagg caaacaggag cattatttca
      601 ctacccgcct gactgattcg acgattgtcg atatgaaact gcatatgccg cattgccagg
      661 acccggcgaa acgcgagttc acccagttgc tcgaagtttc actggcctac cgcaaaattg
      721 aatgggaaca cgtcaaatct ggcacctccg gtgccgacga ctggcgcgca ccgctggaag
      781 cataagttaa gccaacagca tccggctatc aggccggatg ctgtttttgt attacgccgt
      841 agaaagaaag gttgtcagta aggggttacg ttcacgggag atggcggctg ccggaaactt
      901 cggcggtagc gtgcctgaac gcaagcgtaa cactgtactg tcacagccgc tttaacagcg
      961 aactgtctgt ttttccttaa ccccggaggt tgctatgtca accggattac gtttcacgct
     1021 ggaagtggac ggcctgccac cggatgcttt tgcggtggtc tcctttcatc tgaaccagtc
     1081 actctcttcg cttttttccc tcgatctctc tctggtcagc cagcagtttc tctcccttga
     1141 attccagcag atcctcgaca aaatggccta cctgacgata tggcagggcg atgacgtaca
     1201 gcgccgggtg aaaggtgtgg tgacctggtt tgaactgggg gagaacgaca aaaaccagaa
     1261 gctgtacagc atgaaggtat gccc
//
LOCUS       DAZUCE010000471         1281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZUCE010000471 DAZUCE010000000
VERSION     DAZUCE010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.474"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            50..592
                     /gene="ecpR"
                     /locus_tag="ACHY03_005246"
                     /gene_synonym="matA"
     CDS             50..592
                     /gene="ecpR"
                     /locus_tag="ACHY03_005246"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFS3908327.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            667..1254
                     /gene="ecpA"
                     /locus_tag="ACHY03_005247"
                     /gene_synonym="matB"
     CDS             667..1254
                     /gene="ecpA"
                     /locus_tag="ACHY03_005247"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFS3908328.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
BASE COUNT          389 a          278 c          310 g          304 t
ORIGIN      
        1 agtgacatgg caaaatgatt acagcaggga ctatgaggtt aaaaaccata tggaatgtca
       61 aaaccgttct gataaataca tctggtctcc ccatgacgcc tacttctata aaggactatc
      121 tgaactgatt gtggatatcg acagattaat ttatctatcg ctggagaaaa tcagaaaaga
      181 tttcgtgttt atcaatctca atacggattc tttaactgaa tttataaacc gtgataatga
      241 gtggttatcc gcggtaaagg ggaaacaggt cgtattgatt gcggccagaa agtcagaagc
      301 cttagcaaat tattggtatt acaacagcaa tattaggggc gtggtatacg ctggactgag
      361 tcgtgatatt agaaaagaac tggcctatgt gattaatggc aggttcctga gaaaagatat
      421 taagaaagat aaaatcactg accgggaaat ggaaattatc cgcatgacgg ctcagggaat
      481 gctgcctaaa tcgattgcca gaattgaaaa ttgtagtgta aagacagtgt atacccatcg
      541 gcgtaatgca gaggccaagc tgtactcaaa attatataag ttggttcagt aaactccagg
      601 caagttagtt ttaaaaaatg actcactggg acatcacgtc ctcaattcaa ctcgggaaga
      661 aatacaatga aaaaaaaggt tctggcaata gctctggtaa cggtgtttac cggtatgggt
      721 gtggcgcagg ctgctgacgt aacagctcag gctgtagcga cctggtcggc aacagccaaa
      781 aaagacacca ccagtaagct ggttgtgacg ccactcggta gcctggcgtt ccagtatgcc
      841 gaaggcatta aaggttttaa ctcacagaaa ggtctatttg acgtggctat cgagggtgac
      901 tcaacggcta ccgcctttaa actgacctca cgtcttatca ccaacacatt aacccagttg
      961 gatacctcag gttccacact gaatgtgggc gtggattata acggcgcggc agtcgaaaaa
     1021 actggcgata ccgtgatgat cgataccgcc aacggcgtac tgggcggcaa ccttagcccg
     1081 ctggcaaacg gttacaatgc cagcaatcgt accaccgcac aggatggttt cactttctcc
     1141 atcatcagcg gcaccaccaa tggtaccacc gcagtaaccg attacagcac tctaccggaa
     1201 ggcatctgga gcggcgacgt tagcgtacag ttcgacgcga cctggaccag ttaatctctc
     1261 tgatgtacca gcaggggtag c
//
LOCUS       DAZUCE010000472         1275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZUCE010000472 DAZUCE010000000
VERSION     DAZUCE010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.475"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            21..527
                     /gene="yciE"
                     /locus_tag="ACHY03_005248"
     CDS             21..527
                     /gene="yciE"
                     /locus_tag="ACHY03_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS3908329.1"
                     /translation="MNRIEHYHDWLRDAHAMETQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(587..1225)
                     /gene="ompW"
                     /locus_tag="ACHY03_005249"
     CDS             complement(587..1225)
                     /gene="ompW"
                     /locus_tag="ACHY03_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFS3908330.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          365 a          302 c          273 g          335 t
ORIGIN      
        1 tttataaaga ggacattttc atgaatcgta ttgaacatta tcatgactgg ttacgtgacg
       61 cccatgcaat ggaaacgcaa gccgaatcta tgcttgaatc catggccagc cgtatagata
      121 attatcctga actacgtgct cgtattgaac aacatcttag tgaaaccaaa aaccagattg
      181 ttcaactgga aactattctt gatcgtaatg acatttcacg ttcagtcatt aaagattcca
      241 tgagtaaaat ggctgcgctt gggcagtcaa tcggtggtat attcccttct gatgaaatag
      301 tcaaaggttc tattagcggg tatgtcttcg agcaatttga aatcgcctgt tatacttcac
      361 tattagcagc agcaaaaaat gccggtgata cagcctcaat tccgatcatc gaagcgattt
      421 taaatgagga aaagcaaatg gccgactggc tgattcagca tattccgcaa acaactgaga
      481 aatttttaat tcgctctgaa actgatggcg tagaagcgaa gaaataatac cctttatgcc
      541 atgtccttat tgaccccgta tattacgggg tcgtttttgt gcggaattaa aaacgatatc
      601 ctgctgagaa cataaacacc cacggatcga ggcgtacgct atcatgttgc tgtgcaccgc
      661 ccagtttata attggcggtg gtatcgatat ccatgtacca cactgacatg ttaaccaacc
      721 agtcacggtt aatcagataa tcaaccccca cctgcccggc agctccccag gaatctttca
      781 gactgagatc ggaaagcccc gcctctttgc catgatcgtt aaatccatta tcaaagaagg
      841 tggtgtagtt aatacctgcc ccaacataag gacggaattt gctgctggca tcaccaaaat
      901 accactgcgc catcagtgtt ggcggcagat gatgaacggt tgcaatatcg ccggtcgccc
      961 gggtgccgat tttatggcgg aacggcgtcg ctgcaagtaa ttccacacca atgttgtcgg
     1021 tcgccatata agtaaacgtc aggcccagtt gagtgttatt ggtcacgctg aatccaccca
     1081 gacttcctaa cgtaccacca gcaccttctg ttggacgtac ggttgcagaa cctgcacgca
     1141 taaaaaattc gcctgcttca tgggcaaagg cactgccaga gagaagagtt gttactgcca
     1201 aagccgccac tgttaacttt ttcatatccg ctccgtcgtt atggttataa aaacgtgacg
     1261 aatataccta cattg
//
LOCUS       DAZUCE010000473         1275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZUCE010000473 DAZUCE010000000
VERSION     DAZUCE010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.476"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            200..877
                     /locus_tag="ACHY03_005250"
     CDS             200..877
                     /locus_tag="ACHY03_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFS3908331.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            949..1233
                     /gene="ppnP"
                     /locus_tag="ACHY03_005251"
     CDS             949..1233
                     /gene="ppnP"
                     /locus_tag="ACHY03_005251"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFS3908332.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          331 a          276 c          316 g          352 t
ORIGIN      
        1 ttttcataat tcatgttttt caaataatta gaatattaaa caataacaat ccattactgg
       61 aatcatttgg aatctttaca ttatgccgtg cacgtctgct gctacgcttt ttgtcatttg
      121 tagcacaagt aagtgtcagc agtggtgctt aacacttgcc cggtaattaa cgacgaaata
      181 aaagtaaggt ggaagaacaa tgagtgcgtc gttggcgatt ctaaccatcg gcattgtacc
      241 tatgcaggaa gttttgccgc tcctgacaga atacattgac gaagataata tttcccatca
      301 tagcctgctg gggaagttaa gccgcgaaga agtgatggcg gagtacgcgc cagaagcagg
      361 cgaagacaca attctcacat tattaaatga caaccatctg gcccatgttt cgcgtcgcaa
      421 agtagagcgt gacctgcaag gtgtggttga agtgctcgat aatcagggct acgacgtcat
      481 tatattaatg agtacggcaa acattagtag tatgactgcg cgtaatacga tctttcttga
      541 gccgtcgcgg atattgccac cgctggtttc ctctattgtt gaagatcatc aggtgggggt
      601 tatcgtaccg gttgaggagt tgctgaccgt tcaggcgcaa aaatggcaaa ttttgcagaa
      661 accgccggta ttttcattgg gtaaccccat tcatgattca gaacaaaaaa tcattgatgc
      721 cgggaaagaa ttactggcga aaggcgcaga tgtcatcatg ctggattgtt tgggatttaa
      781 ccagcgtcat cgcgatttac tgcaaaaaca gctcgatgtt cctgtcttgc tctctaacgt
      841 attgattgca cggttggctg cggaattact ggtgtaattt tgcgtgacag ccagcgcctc
      901 tggcccctat agtgaagtag atgttcaact accagacagg gccagtttat gcttcaaagt
      961 aatgagtact tttccggcaa agtgaaatcg atcggctttt ccagcagcag cactggtcgc
     1021 gccagcgtgg gtgttatggt tgaaggcgaa tacaccttca gcaccgctga gccggaagag
     1081 atgacggtaa tcagtggcgc gctgaatgtg ttactgcctg acgcgaccga ctggcaagtg
     1141 tatgaagccg gttcggtgtt taatgttccc ggtcacagtg agtttcatct gcaagttgcc
     1201 gaacccacct cttatctgtg ccgctatctg taattcctcg ccttcccctt gaacgggagg
     1261 gcatttttct gaaat
//
LOCUS       DAZUCE010000474         1266 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZUCE010000474 DAZUCE010000000
VERSION     DAZUCE010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.477"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(42..986)
                     /gene="leuO"
                     /locus_tag="ACHY03_005252"
     CDS             complement(42..986)
                     /gene="leuO"
                     /locus_tag="ACHY03_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFS3908333.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          382 a          289 c          253 g          342 t
ORIGIN      
        1 gaataaacca gacattcatg tctgacctat tctgcattca gttagcgttt gcaaattgag
       61 actaattgct cttccatcca ctgatggcct ttatcgcgcc cggcagcttc atgccaggag
      121 agataacagg ttctgctatt tagttttaac ggcagcggta atacctgtaa ttctaaggat
      181 tcagcgaact cttcagccag ccaacgcggc gcaatagcga ccaaatgcgt ttgcgacacc
      241 acgctaagta cgctcatcat tgccatgccc tgatacgcga tactggcttg cttatctacc
      301 gtgtcatacc aaggttgact aaatgacgcg aaacgatcga gcgaaaccgc cgcatgttgt
      361 tcgttataaa catcatgttt cagtaacggg cccttaattg ttgggtgatt tttgctggct
      421 accagcacca tttcatcttt aaataatggc acgctggtaa attcaggacg atggaagtct
      481 tcataactaa tcacaaactc cgtttcctga taacgcagct gatgttcagt gttctgattt
      541 aatgaagact tgaacataac atgtatattt ggcgcaatct gctcaatgtg attataaatc
      601 tgcgaggtca gaatactgtc taacgggctg caaacacaaa gatgaaatac acgttcactg
      661 ctcgcgggtt caaaacctga accaggcaat tcattttgta ctagttgcaa tgcctgacga
      721 actgaaccaa aaagttgaaa tgcgcgagca gtcggttgaa taccacggcc ataacgaaca
      781 aaaagctcgt cattaaacat caccttcagg cgtgcaacag cgttactgac cgcaggttgc
      841 gacattccca gaacatgagc agcacgggta atgttttgct cctgcatcac ggcatcgaaa
      901 acggttaata agttgagatc gaccatgcgt agctgtggct tgctgaactc cgccgtctct
      961 ggatgatctg tttgtacctc tggcatactt aactccactg tcacacttaa ctccctttcc
     1021 cttattggaa tgcagagaaa taatcctcga gaatatgatt tatcatgcat ataaaataag
     1081 aaaaaggaaa atgagtaaaa ttaggaaaac ataaagatac gggttcttat aaaaacagaa
     1141 tccattgccg tggaaagcgt catttaccac agcataataa tccataatgt tcttgttgcg
     1201 taaacaatct aatcataact accgcgaata ctcaatcatc cacaaaatgg attaaatgtg
     1261 cttttt
//
LOCUS       DAZUCE010000475         1255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZUCE010000475 DAZUCE010000000
VERSION     DAZUCE010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.478"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>1255
                     /locus_tag="ACHY03_005253"
     CDS             <1..>1255
                     /locus_tag="ACHY03_005253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908334.1"
                     /translation="KTERGEEKNIKVVVDDTGKYEINLNGWDIKDADGAEVKIVDSAG
                     NVKSGNLYNIARLYVDMNTNKAITLDSAYNIIGSQKEGTDPAKALIMSDDNDWVYIGG
                     GISPHIGDQNPGSDNNIDMAGGDDILSSVGAVLDGANINMGDGDDKLYTQDGFASSGT
                     RNIIMGDGNDVISVDNSFGGKNTISLGEGNNLFIVGNYVNSIAENDITAGSGDDRIEI
                     GTNLDGKNKIDLGDGDNTIQVGGYITNSHTITGNSGDDIIYVATNIDGSGSFNLGEGN
                     NNFIVGGYIQGKNTIEMGSGDDTVSVSTRIADNVKIQLNAGDDSVYAGGLLNKAIVDL
                     GDGDDVVTLSGISDNGKRNNMEELVSTNAMLTGGEGNDTLKINGSFKLLNMKNISGFE
                     TIDLGESSENHLDVGIKSDMLDISSS"
BASE COUNT          409 a          164 c          312 g          370 t
ORIGIN      
        1 aaaacagaac gtggggagga aaaaaatata aaagtagttg ttgatgacac aggaaagtat
       61 gaaataaatc taaatggctg ggacatcaag gatgcagatg gcgctgaagt aaaaattgtt
      121 gattctgctg gtaacgttaa atcaggaaac ttatataata tcgcgcgttt gtatgtggat
      181 atgaatacaa acaaagctat aacgctggat tcggcttata atataattgg ctctcagaaa
      241 gaaggaaccg atcctgctaa agctcttatc atgtcagatg ataatgactg ggtgtatatt
      301 ggtggaggga tatctcccca tataggggat cagaatccag gctctgacaa caacattgat
      361 atggctggcg gtgatgatat tctgtcatct gttggtgcag ttttggatgg agccaatatt
      421 aatatgggtg atggagatga taaactatat acgcaggatg gtttcgcttc ttcaggtacc
      481 cgaaatatta ttatgggaga cgggaatgac gttatttctg ttgataattc atttggtgga
      541 aaaaatacca tttctttagg agagggaaac aacttattta ttgttggtaa ctatgtaaat
      601 agtattgctg aaaatgatat tacggctggt agtggtgatg acagaataga aattggtaca
      661 aatctggatg gtaaaaacaa aattgatctt ggggatggtg acaacactat tcaggttgga
      721 ggatacataa ccaactcaca taccattact gggaatagtg gtgatgatat tatctatgtc
      781 gctacaaata tagatggttc tggctcattt aatttaggtg aaggtaataa caactttata
      841 gtcggaggat atatccaggg gaaaaacaca attgagatgg gttccggtga tgatacggtt
      901 tctgtttcca ctcgtattgc agataatgtt aaaatccaac ttaatgccgg ggatgacagt
      961 gtgtatgcag gtggattgtt aaacaaggca attgttgacc tcggggatgg ggatgatgtc
     1021 gtcacattga gtggaatatc agataatggt aagcgtaata atatggagga gttagtatcc
     1081 actaatgcaa tgcttacagg aggggaagga aacgatactc tgaagattaa tggttctttc
     1141 aaattgttaa atatgaaaaa tatatctggg tttgaaacta ttgatcttgg ggaaagttca
     1201 gagaatcatc tggatgtcgg tataaaatcg gatatgctgg atatttcatc gtcat
//
LOCUS       DAZUCE010000476         1255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZUCE010000476 DAZUCE010000000
VERSION     DAZUCE010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.479"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..504
                     /locus_tag="ACHY03_005254"
     CDS             <1..504
                     /locus_tag="ACHY03_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3908335.1"
                     /translation="NKVITDVGPGKLNLGGCAIEVMGTYNAAIVRVTIGEYGGDGFLN
                     GTVCTCTVYGDTQRFHWRVDYSTKNKPTTASLTVNGWERDEVTGRLRQWGSIEVSEDD
                     GKLMTQHSIRFPVAFPVAALNAQVSAVGDPHAIKMYTLSNPSLVSVTLITVRGCYGKF
                     SWEAIGY"
     gene            514..738
                     /locus_tag="ACHY03_005255"
     CDS             514..738
                     /locus_tag="ACHY03_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3908336.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            738..>1255
                     /locus_tag="ACHY03_005256"
     CDS             738..>1255
                     /locus_tag="ACHY03_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFS3908337.1"
                     /translation="MHIRNFSYYTPAEPDVAGAMYLKSEDGQDWYECQSQFAEDTLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPENRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTA"
BASE COUNT          309 a          262 c          412 g          272 t
ORIGIN      
        1 aataaggtca tcacggatgt cggaccggga aaactcaatc tgggaggctg cgccattgag
       61 gtgatgggga cgtataacgc agccatagtc cgggtcacca tcggcgaata cggtggcgat
      121 ggttttctga acggcacggt ctgtacctgt acagtttacg gagacacaca acgttttcac
      181 tggcgggtgg attacagcac aaaaaacaag cccacaacag caagcctgac agtaaatggc
      241 tgggagcgtg atgaagtaac cggtcgtttg cggcaatggg gcagtattga ggtaagtgag
      301 gatgatggta agcttatgac acagcactct atccgttttc ctgtcgcttt tccggttgct
      361 gctctgaatg ctcaggtgtc tgcagtggga gacccccacg ccattaagat gtataccctg
      421 tcgaatccgt cattagtgtc tgtaactctg ataacagtca ggggatgcta cggcaagttt
      481 tcatgggaag cgatcggata ctgagggcat tgtatggaac agtatgtttt ttcaccgtca
      541 gagaatatgt tctatcccct gtccctgcgg ccggtttatg aggcagcagg gcgctggccg
      601 gaagacggta ttgtggtgga ttatgtggtg tataaagtct ttgcagcgga tgctgcaccg
      661 gcaggtatga agcggggcgt cggtgcggaa aaaatgccgg tctgggtgcc ggtatcggag
      721 gagggcacgg aaacatgatg catatcagaa atttttcgta ttacaccccg gcggagccgg
      781 atgttgcagg cgcgatgtac ctgaagtctg aggatggtca ggactggtat gaatgtcagt
      841 cacagtttgc agaagacaca ctgaaggtgg tgtatgacag ccggggtgtg attacgggat
      901 acggtaagga cactgcgtta ctgtggccgg tgaaccagag tgtggcagaa gtgccggata
      961 ccccggagaa ccggaaaatt gacctgagcg ggcgctgggg ttttgacggg gagaaaatca
     1021 cggacctgct gaccgcggag aaagcgcgag ggatgaaggg cgatgaaatt aacgcctggc
     1081 gtaatgcgat ggaagcggcg aactacacgt ttgagcacaa tgggcgtaaa tgggactacg
     1141 ggaagtcaac gcagacgcgt cttgagccgt cggtggctgc agcgaaagcg gggaaactgc
     1201 cggaggcgtt tttctggacg gatgcggaaa acaatgatgt gccggtgaca gcaga
//
LOCUS       DAZUCE010000477         1238 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZUCE010000477 DAZUCE010000000
VERSION     DAZUCE010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.480"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..257)
                     /locus_tag="ACHY03_005257"
     CDS             complement(<1..257)
                     /locus_tag="ACHY03_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFS3908338.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKR"
     gene            complement(254..478)
                     /locus_tag="ACHY03_005258"
     CDS             complement(254..478)
                     /locus_tag="ACHY03_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFS3908339.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            544..899
                     /locus_tag="ACHY03_005259"
                     /pseudo
     CDS             544..899
                     /locus_tag="ACHY03_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(1132..1236)
                     /locus_tag="ACHY03_005260"
                     /pseudo
     CDS             complement(1132..1236)
                     /locus_tag="ACHY03_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
BASE COUNT          356 a          315 c          267 g          300 t
ORIGIN      
        1 cgttttaaaa aaacctgtgc aacaaacaag gctgttcgct tatttgcatc gttgaaaatg
       61 tgaccacgag caatagcaac taaatacacg gcagccagat ccataatatc tgtcacacct
      121 tcataatgat acatgttcaa aacacgatga atgatcgcgt cagcgcgacc gggttctggc
      181 atacccgcca cgccaccatc acgcgaaatc aagcggtcat gaaactcaat catttcctgt
      241 gaactaacga atttcatcgc ttagaaagct ccgtataggc cgtcccatga ctttgcatga
      301 tctcagcaaa ctcagcatca agagccgctt tacggtatcg ttcaaaagcg tctttatcaa
      361 ccagtacagc cgcgccacct ttacggcgct gaatctcaac aggctgattt tcatcaaccg
      421 ccagctcgag caacgaggcc aggttatcgc gagcatggct atagttacat gtaaacatag
      481 tgcttcctct ttgagttgta caattaaatt gtacaacagt tatcttcaat atgaaatcac
      541 cgttaccgct ggcgcatcct gacccaactt aaccggggag aaagtcgcca tgccgttgcc
      601 agagctatct gtcacggtca gaaaggtgag ataagaaagc gatataccga tggtcaagaa
      661 gatcaactgg gcgcactggg gctggtgact aacgccgtcg tattatggaa cactatttat
      721 atgcaggctg ccctggacca tttcagggca cagggggaaa tcgtgaatga tgaagatatc
      781 gcacgcctct caccactttg ccacggacac atcaatatgc tcggccatat tccttcacgc
      841 tggcagaact ggtgactaaa gggcaaaaag aggcatcgga ggaagaaaac attgcttaac
      901 gtgagttttc gttccactga gcgccagccc cctaattctc tatatccaaa ctgaccccaa
      961 taggtgacgg gttatgtgac gaaatctgat gtagaaatcg ttgtttcagt gacagtcact
     1021 caatcggtca tttatgtgac accccacgcc gttactggtc gcggaaaaat ccagcggaaa
     1081 aatccaggtt tccggcttgg gaccccatct caagagatcc tttgatcttt tctactgaac
     1141 cgcgatcccc gtcagttcag aagacgaaga tgacgcaacg gttcctcatt gtacatcacg
     1201 aatatagttt gcttgacatc ctgacaagaa taatattt
//
LOCUS       DAZUCE010000478         1230 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZUCE010000478 DAZUCE010000000
VERSION     DAZUCE010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.481"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            1..77
                     /locus_tag="ACHY03_005261"
                     /pseudo
     CDS             1..77
                     /locus_tag="ACHY03_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            375..722
                     /locus_tag="ACHY03_005262"
     CDS             375..722
                     /locus_tag="ACHY03_005262"
                     /inference="COORDINATES: protein motif:HMM:NF014559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     determinant, SpaN/EivJ family"
                     /protein_id="HFS3908340.1"
                     /translation="MFTQHIEKQRNVHTLNQNDINNSVNSVNVPENELTYQFQRWGQN
                     HTVRILESSEGIRLKPSDALVSDRLHEAQNNDVTAQRWVLTEQDERQGQRHQPHEEQE
                     NEGKFENDHKDES"
     gene            682..1230
                     /locus_tag="ACHY03_005263"
                     /pseudo
     CDS             682..1230
                     /locus_tag="ACHY03_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT          448 a          209 c          259 g          314 t
ORIGIN      
        1 agactcagga tagccaattt tctacgttgg ggaaaaaaaa tgggcaaatg aagtctattg
       61 acaacaaatc atctctccag gttgacagga atccatatct ttctttgcaa acaaatggaa
      121 cagagcaatt ttctacaaag aaaattctca atatgacttc tcgaggtcag ggtattagtt
      181 ctgaactatc tgataacacg ataaaaatta aggaaaaaat ccataatggt agccatacag
      241 agtacatcac agatcaaaaa aacagtaata ataaagatcg tgagaaaaaa taccgcgatg
      301 gagacaaaat aaatggaccg caggctcatt cattagacag tactaacgaa agaaggtttg
      361 ctgataacag aaccatgttt actcaacata tcgaaaagca aaggaatgtt catacgctca
      421 atcagaatga tataaataat tccgttaata gtgtcaatgt gccagaaaat gaattgacat
      481 atcagtttca gcgttgggga cagaatcata cagtccggat tctggaaagt agtgaaggga
      541 tacgactaaa accatcagac gcgcttgtct ccgacagact ccatgaagcc caaaacaatg
      601 atgttacagc acaacgttgg gtattgactg agcaagatga acgacaagga caacgacatc
      661 agcctcatga ggagcaagaa aatgaaggca aattcgaaaa cgatcataag gatgaaagtt
      721 aatgtttggt ctgaggaaag taaatcgaaa cactcatacg tttgaaacca cttttcaaaa
      781 ttggaaagaa aatggcgaag atgttgcatt attaatgccc gaatttagtg ctaaatggct
      841 accaatagca gaagagagtg gcagctggtc tggatgggta ttgcttcggg aaatatttcc
      901 cttaatttct tctgagctcg ctgggatggc tttaatgcca gaaatggaaa ggttaatagg
      961 ggaatggctc agtttatcca gttccccgtt aaatctaaag catcctgaac taaaatataa
     1021 tcgtttgtgc gtaggtaaag tatttgatgg agtattgagc ccagctcaac cactaataag
     1081 aatatggaca ggggaattga atctttggtt agataaagtc acagtctgcc aatacggaaa
     1141 cgctccagcg ttagacaaaa aatcattata ttggcccatt cattttgtga ttggatttag
     1201 taaaacatgt tatagaacta ttgttgatat
//
LOCUS       DAZUCE010000479         1214 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZUCE010000479 DAZUCE010000000
VERSION     DAZUCE010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.482"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            136..738
                     /gene="ais"
                     /locus_tag="ACHY03_005264"
     CDS             136..738
                     /gene="ais"
                     /locus_tag="ACHY03_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFS3908341.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(777..1202)
                     /gene="nudI"
                     /locus_tag="ACHY03_005265"
     CDS             complement(777..1202)
                     /gene="nudI"
                     /locus_tag="ACHY03_005265"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFS3908342.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          324 a          278 c          258 g          354 t
ORIGIN      
        1 catttggcaa aattttgagg cattttgccg gcatcgcagg atttttaata ttacaccaac
       61 tgctaattat cgccagaaat gtcgctcaat ttcacggtaa ttgtctggtt gcgcttgtct
      121 ataggtggag tttacgtgtt agctttttgc cgctcttcgt tgaagtcaaa aaaatatttc
      181 atcattttac tggcgctcgc tgcaattgcc ggactgggta ctcatgccgc ctggagtagc
      241 aatggtttgc cacgtatcga caataaaaca ttggccagac tggcacagca gcacccggtt
      301 gtcgttttgt ttcgccatgc tgaacgttgc gatcgttcaa ccaatcaatg cttgtcagat
      361 aaaacaggta ttacggtcaa aggtacccag gatgcccgtg aactgggcaa cgcttttagt
      421 gctgatatcc ctgatttcga tctttattcc agtaataccg tccggaccat tcagtccgcg
      481 acctggtttt cagcgggtaa aaagctcacg gtagataaac gacttcttca atgcggtaat
      541 gagatttata gtgcaattaa agacttacaa agaaaagcac ctgataaaaa tatcgttatt
      601 tttacccata atcattgcct gacatatatt gctaaagata agcgtgatgc gacatttaaa
      661 cctgattatc tggatggttt agtcatgcat gtggaaaaag gcaaagttta tctggatggg
      721 gaattcgtta accactaaat tttcaatctg acagccagtg atggctgtca tcattgttaa
      781 agaagacctt tcaaacgtaa cgtttttcgg gtggcaacat tcaaatcata atgcaccaga
      841 tcttcaggtt ttacccacgc gtagtcctga aactcttcgt ttattttcac ttctcggttg
      901 gcagaaacgc agtcaaaaat caggtaaatc atataaatct cttccttgcg accatctgca
      961 tacgtcttgg tgcgaatatc atcgctgaag gtccatggcg tgatttctgt caaaagcagc
     1021 tgttctccca attcttcgcg aatttcgcgg cgtagtgcct cttcaattcg ttcgccgggc
     1081 tccacgccgc cacccgaaag cgcccattga ccggggaaaa cgccgcgatc gtcggccatt
     1141 ttacacagca aataagcacc atcattttga atcaaagggc atacaatagt ccgttgtcgc
     1201 acaggggtat tcct
//
LOCUS       DAZUCE010000480         1211 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZUCE010000480 DAZUCE010000000
VERSION     DAZUCE010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.483"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(160..>1211)
                     /locus_tag="ACHY03_005266"
     CDS             complement(160..>1211)
                     /locus_tag="ACHY03_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFS3908343.1"
                     /translation="VAHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGDKTYARLGF
                     KGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISEFGSLDYGRNYGV
                     GYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDFFGLIEGLNFAAQYQGKN
                     ERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNAS
                     GKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLR
                     PSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVD
                     IRTDNIVATGITYQF"
BASE COUNT          343 a          265 c          266 g          337 t
ORIGIN      
        1 caataaatat tatagtaaga tgtccccgga gggtatggaa taatattaat tgaggtgaat
       61 gaccagaaat atcaaaaatt gctcattttt acttaaggag tcatacagca gcacttaaat
      121 caaaaaccag cccagagaag ggctggtcag gagaggatgt tagaactgat aggtaatgcc
      181 cgtagcgacg atgttatcgg tgcgaatatc tacggcacgg gtaaagtcgc tttcatcaat
      241 caggttgatt ttataatcaa cataggtcga catatttttg ttgaagaaat acgtcgcacc
      301 aacgtcgata tactcaatca tgtcctggtc gccgtagcgg cccagatctc tgcctttcga
      361 tttcaggtaa gcgagtgagg ggcgcaagcc gaagtcgaat tgatattgcg ccaccacttc
      421 aaaactttgt gctttgttag aaatataacc atcgccatag gtggtcatgt ttaatgtttc
      481 agcgtaatta gctgcaaagt agatgttgtt ggcatcatat tttatacctg tagcccacag
      541 ttctgcattc ttaccggagg cattgagagg attagcagcg gctctttcct gcgcatttgt
      601 ccgatcggat ttggtatata ccgcaccgat accaaagcca tcataaacat aagttgagga
      661 gataccgaaa ccgtcaccgt tggcacgcgt gtagtcagca ccataaagat gactgttgtc
      721 agtacgttca tttttgcctt gatattgcgc ggcaaagttc agcccttcaa ttaagccaaa
      781 gaaatcgtag ttgcgatagg ttgctacacc ggtggtacgg tatgtcatga agacatcttt
      841 ctgactccag gtatcgccac caaattctgg caacatatcg gtccatgaac caatgtcata
      901 gccgacaccg tagttacgac catagtccag agagccaaat tcactaattt ttaaaccagc
      961 ataggccaga cgggtcttgt tgccgcgcga gccgttggct tcatcgttat agcctttaaa
     1021 atcatattcc cagtgaccaa aaccaatcat ttgatcattg atttgcgttt ctcctttaaa
     1081 gccgagacgg gcataagttt tatcaccatc gtcacgctta tcatcagtaa aataacgtag
     1141 agcggtaacc ttgccataaa ggtcgagttt attaccatcc ttgttataga cttcggcagc
     1201 gtgtgctacg t
//
LOCUS       DAZUCE010000481         1210 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZUCE010000481 DAZUCE010000000
VERSION     DAZUCE010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.484"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..423)
                     /locus_tag="ACHY03_005267"
     CDS             complement(<1..423)
                     /locus_tag="ACHY03_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFS3908344.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQLS"
     gene            complement(423..752)
                     /locus_tag="ACHY03_005268"
     CDS             complement(423..752)
                     /locus_tag="ACHY03_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3908345.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKRY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(749..>1210)
                     /locus_tag="ACHY03_005269"
     CDS             complement(749..>1210)
                     /locus_tag="ACHY03_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFS3908346.1"
                     /translation="VGRIGSAIGGAFGGGASASSGTAIEAAAANFHFATGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFN
                     QNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSGSGR"
BASE COUNT          250 a          465 c          256 g          239 t
ORIGIN      
        1 tgataactgc tccaccaccc accggtccgt cagctcctgc tccgggtctg cctccggatt
       61 ccccgccaca aagttcaccg catcaagaaa acgcgcatac acccggcggc ggaccaccgt
      121 ggcacccacc aggctctgca aatcctccgc catcccggtg acaaggccga aaagattgga
      181 caccgtcagc gacgggcggg cactgctgcc ctttccgttc atctcaaagc cgctgccctc
      241 aatcgggtac gcctcatact tccgcccctg ccaggtcacc ggctcccctt tttcattcag
      301 ctcattgcag aaaaaatacc gctcaccgcc ctgcaccgtc aggtcgattt cccagagcac
      361 cacccgcggt gactgctctg acttaaccga ctcgttcagg cttgcttcgt gaatatcctg
      421 catcagttca ccacctgctt aaactccgcg ctgaactcaa cgcgcaacat cccgacccgc
      481 gcagaccacc cggcgcaggt cacctttatc tgccggtatg catagggtgg cttccacaaa
      541 aatgccttcc agcccccgtg ctctgccaga aacgcttcca gatgccgggc ctcctcccgg
      601 gtcacggaaa gcatcaccct gtacctcttc aggtctgcat tcagccctgc cgccatacgc
      661 tgtgagtacc cgtcaccaaa acgcacttca cgcaccgacg gctgcgagtt cacctccata
      721 tccggcttca ctttccagcg aaaggttttc atcgcccgct ccctgataac ataccgccat
      781 cacgcaactg cagccggagt tcatcctgcg cccccttacg ggccatgtca tacaccgcct
      841 tcatcagctg cggccccgcc tgtccgttta taccgtcgtt ctgaatcacc acgtgattgt
      901 tctgattaaa attaatacct tccgcacgcc gcatctgcgc cggacttccg gcaccaccca
      961 catacccccc ttccgcatag ccgcgcatca gacggtaaag attccccaca cctatccggc
     1021 tggttgcctc cttcgtgaaa acaaactccc cgcggtgaac tatccccgca ggctcatatt
     1081 tgccgcccgt ccccgtaaat cctccggtcg cgaaatggaa gttcgccgcc gcagcctcaa
     1141 tggccgtccc cgaggaagca gatgcaccac caccgaaagc accaccaatg gcgctgccga
     1201 tacgcccgac
//
LOCUS       DAZUCE010000482         1187 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZUCE010000482 DAZUCE010000000
VERSION     DAZUCE010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.485"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..204)
                     /gene="mchB"
                     /locus_tag="ACHY03_005270"
     CDS             complement(<1..204)
                     /gene="mchB"
                     /locus_tag="ACHY03_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFS3908347.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALARIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSAS"
     gene            complement(232..441)
                     /gene="mchI"
                     /locus_tag="ACHY03_005271"
     CDS             complement(232..441)
                     /gene="mchI"
                     /locus_tag="ACHY03_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFS3908348.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
     gene            853..1098
                     /locus_tag="ACHY03_005272"
     CDS             853..1098
                     /locus_tag="ACHY03_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908349.1"
                     /translation="MNCDNNHRNEEFIVTFDKGNKQDNSRRKHDNFPIEVESSVEQET
                     HCITNNKSASGIVTHDYDADYICGCGEIMCPGCGHDL"
BASE COUNT          394 a          252 c          219 g          322 t
ORIGIN      
        1 actggcacta ccacttccca cggtcgagcc aattgctgtt gtcaaaccgg cagatacggc
       61 tccaactaca accccaagtg gaccaccagg tattctggcg agagctccaa caatagctgc
      121 agcacctgca gcattagctg aagtcgctgg cgcacctccc gccccggaaa tatatcttaa
      181 ctgtgattct gttatttctc gcatataaac tgcatataaa ctccatcata tttaacttcc
      241 gtttttccag cctgaccaga ccagaatcct tcgaatccca tgaatcagct caataaaacc
      301 aagaaatatt ataaagctta gaaaaacgtc aacataagaa ttcccttcgc caacaatccg
      361 aagggatgaa agtgaaacca ataagatagt aataggtaaa ctgaatatag ccgtcaattg
      421 ggacagtttt ttataactca ttaatcccta cctgttgctt tattttcatt atgaaaagtt
      481 accaaatctt agcacctcag tcaggagagt caggtcatcg ataaagaata ttccagtaga
      541 gtggcgctca tgatcaaaga taacagcaaa gttgatgaca gcgctgaccg actgtcattt
      601 acagaaaccc tgtttgtagc acattccatc ttacctgacc tcacccagac aaagacaaaa
      661 gagaatgatt gttatttact tttcaaaaaa agtgtcatat tgtccatata aagtcaagag
      721 gtattttacg ttatgtcact gatgcaattg ttagtcagga tctcatggtt gtcgctcatg
      781 tataacctga gacttgaacg cacactaata gcactgacgg tcatacatcg ggaaatgtga
      841 ggaggcataa gaatgaattg tgataataat cacagaaatg aagaattcat tgttaccttt
      901 gataaaggca acaagcaaga caattcaaga cgaaaacacg ataattttcc tatagaggta
      961 gaatcctccg tagagcagga gacacactgt atcacaaata ataagtcggc ttccggtata
     1021 gtaacacatg actatgatgc cgattatatt tgtggttgtg gtgaaattat gtgtcctggt
     1081 tgcggtcatg acctataaca aacatttttg caaactgata cctgatcagg tacaagcctg
     1141 ttgattatta tgctgaatat ttcatgttat aaaaccatcg atataaa
//
LOCUS       DAZUCE010000483         1179 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZUCE010000483 DAZUCE010000000
VERSION     DAZUCE010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.486"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..1102)
                     /locus_tag="ACHY03_005273"
     CDS             complement(<1..1102)
                     /locus_tag="ACHY03_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFS3908350.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATD"
BASE COUNT          347 a          285 c          210 g          337 t
ORIGIN      
        1 tatcggttgc aactccagcc tttttggtaa attcgctttc atcaataagg ttaattttgt
       61 aatcaataag aagtgacatg tttttgttca gaaagtagtt tgcgccaaca tcaatatact
      121 caacaagaga ctgatcgcca tagtttacta ttccaccttt aacatccatc cctttggact
      181 gaacgtaagc cagtgaagga cgcaggccga aatcaaacat atactgagcc acaacttcaa
      241 tattccgtgc tttatctgcg atgcctgcgc taccaaatgg agtcatattt cgggtttcag
      301 agtagtcagc tgccagatac aggtcattat cattatactt gaccccaaca cctgcaaact
      361 ccgcatgttt tcccccggca atccagcgag gcgtaataac ttcttttcct tctgaatctt
      421 tcgatgtcat gaaacgttta cggccttctt tttcctgcag actggttctg tcggcattag
      481 tgtaagcggc aacgattcca aaaccttcat agtcatagct tacggagaaa ccatatccat
      541 caccattagc ttcaagtaaa ttatcacggt catttttacc ctgatattga gcagaaaaag
      601 taagtccatc aacaagcccg aagaaatctg tattgcgcca ggtcagaact ccggttgttc
      661 tgccggtcat atagttatca gagtaagtcc agctgtcacc accaaagtcc ggaagtacgt
      721 cagtcagagc ccccacatca taggcaacac catagtttcg cccataatca agtgaaccga
      781 gtatattgtg gcgaatacct gcaaatgcca gacgattttt atcgttgcgt acttcacttt
      841 ctgtcttaaa ccccaggaac tggtattccc actgaccaaa gccagtcagg ctctcattaa
      901 tttgtgtttc gcctttgaaa ccaagacgtg caaatgttct gtcgccatcc atagaaccat
      961 cagagatgta atgacgggct ttaacgcgac cataaaaatc cagtttatta ccgtctttat
     1021 tatagatttc tgctgcctgt gttgcctcac acagcattaa tgcagaaacg acagagcaca
     1081 caaccatact taattttcgc ataaaaaaat cccctgtgca catttttagt atggttttaa
     1141 aaattaaact atggcgaaaa aattatcact atgcaaaga
//
LOCUS       DAZUCE010000484         1174 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZUCE010000484 DAZUCE010000000
VERSION     DAZUCE010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.487"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1160
                     /locus_tag="ACHY03_005274"
     CDS             <1..1160
                     /locus_tag="ACHY03_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFS3908351.1"
                     /translation="FMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGST
                     LIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFP
                     LLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASEL
                     WPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLS
                     SKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDK
                     VLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAEHMRKNVELLTGFSN
                     RYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT          324 a          237 c          232 g          381 t
ORIGIN      
        1 ttttcatgtt aatgaaatat ttcactaaca aacaaatttt accaacactc attttaagcc
       61 ttgcattttt aagtggcctt atctatttag ttgaaaccat tgtaattatc cataaaccaa
      121 ttaacggcag tacactgatc cagacaaagt cgaatgatgt ttctattttc tatattttcc
      181 gccaactcag ttttatttgt ttaacctcgc tggcgctctt ttgttatgga aaagacaaca
      241 tccttgacaa caataagaaa aaaactggca ttctgttgct ggcgctgatt ccttttttag
      301 tttttcccct tctggcacac aatctgagca gttataacgc tgactattct ttgtatgtcg
      361 tcgattactg tccggacaac catactgcga cctggggaat caactataca aaaatattgg
      421 tttgtctgtg ggcattttta ctgttcttta ttatcatgcg tacacgatta gccagcgaac
      481 tatggccgtt aatagcatta ttatgtctgg catcgctatg ctgcaactta cttctactga
      541 ctctggatga gtataattat accatctggt atatcagtcg cgggattgaa gtctccagta
      601 aactgtttgt tgtgtctttt ctgatttata acatttttca ggagctgcaa ctctccagca
      661 aactggcagt tcatgatgtg ctgaccaata tttataatcg gcgctacttt ttcaacagcg
      721 tagagtcatt attgtcgcga cctgttgtta aggacttctg tgtcatgctg gttgatatta
      781 atcagttcaa acgcatcaat gcccaatggg gacatcgtgt gggtgataaa gtgctggttt
      841 caattgtcga tattatccag caaagcatcc gccccgatga tattttagcg cgactggagg
      901 gtgaggtgtt tggcttgcta tttaccgaac tcaatagtgc ccaggcaaaa atcattgcgg
      961 aacatatgcg taaaaatgtc gaactcctga ccggctttag taacagatat gatgttcctg
     1021 aacaaatgac catcagtatt ggcacggttt tttcaacggg tgacacgcgt aatatctcgc
     1081 ttgtcatgac ggaagcagat aaagccttac gcgaagcgaa aagcgagggg ggcaacaaag
     1141 tgattattca tcatatttaa gtgcaaaaat attc
//
LOCUS       DAZUCE010000485         1173 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZUCE010000485 DAZUCE010000000
VERSION     DAZUCE010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.488"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            155..262
                     /gene="yniD"
                     /locus_tag="ACHY03_005275"
     CDS             155..262
                     /gene="yniD"
                     /locus_tag="ACHY03_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFS3908352.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(315..1073)
                     /locus_tag="ACHY03_005276"
     CDS             complement(315..1073)
                     /locus_tag="ACHY03_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFS3908353.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          330 a          284 c          270 g          289 t
ORIGIN      
        1 tatatcgata cataatttta ttttttcata aaacgcagaa acagtgatca ggtgtacaac
       61 gccacccgac ggctgtaata acaaaaaatc ccttgatgcc tgtccctttt gttacactcc
      121 gttatcacgc acaagagata tgcaggacac tggtatgccg actaaacgct ttgataaaaa
      181 acactggaag atggtggtgg tgctactggc aatctgtggc gctatgttgt tgctacgttg
      241 ggcagcaatg atttggggct gagcagtgac aaacgaaagc agccagaccg cataacacga
      301 tccggcagat aattttacat gctgtagccc agcgataagg ttgtccgacg atcggtatgt
      361 tccggtgcgg actctggagg ttcagagttc catgtgacgt tgtaagccac cttcagtcca
      421 aaatgttcat taatagcaac atttaatgca ctttcggagt tcagcgttgt gtcttccgcg
      481 ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag tcaactgcca tgcataagcc
      541 ccggaggcat agcccagcgg ctgggtttca ctggcattgt cggtatattt gtcgtaacgc
      601 acacctggac cgaattcaaa gcggaaactg tgtaccgggc cattcaaaaa ctgacgacca
      661 taacccgcgg tcaacacatc gcgctctcga tagccgttat aacggtctgt cagccagctt
      721 gcctgaccaa ataaatagtc ataatcagtt aaattaaaac ggctacgccc gcccgccgca
      781 tatttttctg aagaacgctc atcattagaa gaagtattac tggcgttccc ccacagcgac
      841 caggcagtgg tttgtccata ccaggtcatg gtggtgtcag ccgtaagtga ggagcttttc
      901 gtattgcctg attgtgcaag atatcctgcg ttcagattac cttcgaaagg ttttttagcg
      961 ctggcaggat cgtccatgac agtaaaaacg gaatcatcgg cagctgcatt cagtgacgca
     1021 aacatgcccc ccgccaacat aacgatggca ggaactgtct tcaaaagctt catttatcaa
     1081 gagtccgtac aacaaaaaaa gagaccatcg cggtcccgga aactttctta aggatcaaag
     1141 attagcgtcc ctggaaaggt aacgaattat aaa
//
LOCUS       DAZUCE010000486         1169 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZUCE010000486 DAZUCE010000000
VERSION     DAZUCE010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.489"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..287)
                     /locus_tag="ACHY03_005277"
     CDS             complement(<1..287)
                     /locus_tag="ACHY03_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908354.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPGGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNA"
     gene            complement(309..848)
                     /gene="yhaB"
                     /locus_tag="ACHY03_005278"
     CDS             complement(309..848)
                     /gene="yhaB"
                     /locus_tag="ACHY03_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFS3908355.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQSDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAYFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1104..>1169)
                     /locus_tag="ACHY03_005279"
     CDS             complement(1104..>1169)
                     /locus_tag="ACHY03_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFS3908356.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          354 a          207 c          225 g          383 t
ORIGIN      
        1 tgggcgttat aacctaaaaa atccagacga agagacgagt cattataaag aattacattt
       61 tgtaagtacg ctaattcttt ttgaaaatca ccagtgataa tgcattttcc accaggttgt
      121 aacgtgttaa gtaaggtgac catttcaata gaggtctgtt ttagtttttc tatacctggt
      181 gctagtgctt cgaggttatt tcctgttggg ctttcaggaa ggtcattcat ttttcgacgg
      241 actaaatcac tactaatatc attaccaatt gatgaaacag ggaacattat tttatccttt
      301 ataaataatc aataaaaatc attattaaaa tgatcgggtg aaaaaaagag aatttgccaa
      361 caatctgaag taatgctggt tttattaaag tacgcagggg gtgttaagat ggcttctttt
      421 cgggtgaatt ttgcacaata cttagaaatc gtctccttaa tacattccag ctctttttta
      481 ttgacggctg agcaagtaca tttttcgcca aaaggtgcaa aagtgcctac atctaatgtt
      541 tgtatgatat aatcgtcatg ttctcctgta gaaatatcta tgatgaatgg actcatattt
      601 tccggttgga ttcggatcat gacagaagga acatcgcttt gatagaaatt caatgcagta
      661 attgcagata atactctccg cagttcatta cttatactgt aaattttatt aacgtcattg
      721 gtggagacaa tttctgcaaa ctttcttttt accgtccaat aatcaatatt tctattgtga
      781 ttgttaacca ctgcgtgcat tggcgggatt gaagggtgaa aaatatgtgc aattgggaag
      841 cctttcattg atgcctgcct tgtcgtcatg acaaacaata atagatgctt attttgctcg
      901 cagggttctt tcgcactatc aaaaaacgct catttacccg gcgttacagt aaacgaagca
      961 acattctgtg gcctacgccg atcaaaagct ctgtgaaaat ttcccttgtt gtctttgtct
     1021 gaccttatcg cggcgatcag caattattcg ttttgtgatc ctcaacacaa aatgctattc
     1081 gtcgcctatg aggggagaag aatttatgga aaacgattgc attcacaaaa tgacgtcctg
     1141 tcagaagaga aaaagttgac gttaatcaa
//
LOCUS       DAZUCE010000487         1157 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZUCE010000487 DAZUCE010000000
VERSION     DAZUCE010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.490"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>1157
                     /locus_tag="ACHY03_005280"
     CDS             <1..>1157
                     /locus_tag="ACHY03_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFS3908357.1"
                     /translation="NWQKKKIERSIKYQTRVFNEHINELVITRRNGKVITVNDGREMI
                     EFVSCSYLGLETHENLKHAIANAIERFGVQLSVARTRVKVDLFEQLEKRLNKIMNGAH
                     TLTFNAVTPCHLAVLPLLASGTLPNYKFNKKPYFILEKTAHATLQINRGLLQQFGETI
                     RVDFQHKNDIEEAFKYASINNMVPISISDSVGSMGGVAPVEFIVRMANRFDGYAYIDD
                     AHGMSIYGKNGSGYVMSCLDNKLDDRIIIAGSLSKAFGSHGGFIACNYPDTINFIKTY
                     GTTYAFGGPPSLPGIAACIAAADLHLDGTVSLRQNDLQQVIKYFDDKFNETEVINKGT
                     TMPIRGLLIGNEEKAITICKKMHDRGFATTVAMYPTVEEGKAILRCAISAI"
BASE COUNT          406 a          189 c          232 g          330 t
ORIGIN      
        1 tgaattggca aaagaaaaag atagaacgtt caatcaaata ccaaacaaga gtatttaacg
       61 aacatataaa tgaactagtg atcaccagaa gaaatggtaa ggtaatcact gttaatgatg
      121 ggagggagat gattgagttt gtgtcatgct cttatcttgg actggaaaca catgaaaatc
      181 taaaacatgc aatcgctaat gcaattgaaa gatttggagt ccaactttcg gtcgccagaa
      241 cgcgcgtaaa agtagattta ttcgagcaat tggaaaagag attaaataaa ataatgaatg
      301 gggcgcacac attaacattt aatgcggtta ctccatgcca tttagctgta ctcccacttc
      361 ttgcatcagg aacactacct aattacaagt ttaataaaaa accatatttt attttagaga
      421 aaacagcgca tgcaacattg caaataaata gaggactatt acaacagttc ggtgaaacaa
      481 tcagggttga ctttcaacat aaaaatgata tagaagaagc gtttaaatat gcttcaataa
      541 ataatatggt accaatatct atatctgata gtgtcggttc gatgggtggg gtggctcctg
      601 tcgaatttat tgttagaatg gctaacagat ttgatgggta tgcttacatt gatgatgctc
      661 atggcatgtc aatatatggg aaaaatggct ccggatatgt aatgtcttgt ctggataaca
      721 aacttgatga cagaattatt atcgctggtt cattatctaa agcgtttggc tctcatggtg
      781 gatttattgc atgcaattat cctgacacca taaactttat aaaaacgtat ggaactacct
      841 atgcttttgg tggtccacca tcattaccag gaattgctgc atgtatcgca gcagcagatt
      901 tacacctcga tggcacagta tccttgcgtc aaaatgactt acaacaagtt ataaaatact
      961 ttgatgacaa atttaatgaa accgaagtca taaacaaagg gactacaatg ccaatacgtg
     1021 gattgttaat tggcaatgaa gaaaaagcta taacgatatg caaaaaaatg catgatcgtg
     1081 gttttgctac aaccgttgct atgtatccta cagtggaaga gggaaaggca attctacgtt
     1141 gtgcaatatc agctatt
//
LOCUS       DAZUCE010000488         1145 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZUCE010000488 DAZUCE010000000
VERSION     DAZUCE010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.491"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            20..>1145
                     /locus_tag="ACHY03_005281"
     CDS             20..>1145
                     /locus_tag="ACHY03_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFS3908358.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDCFYCRHRHYQAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKYWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRSYWRKQLYKVTQRKVLPR
                     LMNELDQLYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGGRVIYVHDLPMPGLLRHAKAVVTINSTVGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFR"
BASE COUNT          314 a          244 c          269 g          318 t
ORIGIN      
        1 actattacgc aaattattta tgcaaggtaa tgcactaacc gttttattat ccggtaaaaa
       61 atatctgcta ttgcagggac cgatgggacc ctttttcagt gatgtagccg agtggctgga
      121 gtcattaggc cgtaacgctg tgaatgttgt attcaatgga ggggattgtt tttactgtcg
      181 ccaccgacat tatcaggctt actaccagac accgaaagag tttcccggtt ggttacggga
      241 tctccaccgg caatatgact ttgataccat cctctgcttt ggtgactgcc gcccattaca
      301 caaggaagca aaatattggg caaagtcgaa aggtatccgc tttctggcat ttgaggaagg
      361 gtatttacgc ccgcagttta ttaccgttga agaaggcgga gttaacgcat attcatcgct
      421 accgcgcgat ccggattttt atcgtaagtt accagatatg cctgcgccgc acgttgagaa
      481 cttaaaacct tcaacgatga aacgtatagg tcatgcgatg tggtattacc tgatgggctg
      541 gcattaccgc catgagttcc ctcgctaccg ccaccataaa tcgttttccc cctggtatga
      601 agcacgttgc tgggttcgtt catactggcg caagcaactt tacaaggtaa cacagcgtaa
      661 ggtattgccg aggttaatga acgagttgga ccaactttat tatcttgccg ttttgcaggt
      721 atataacgat agccagattc gtaaccacag taattataac gatgtgcgtg actatattaa
      781 tgaagtcatg tactcatttt cacgtaaagc gccgaaagaa agttatttgg tgatcaaaca
      841 tcatccgatg gatcgtggtc acagactcta tcgaccatta attaagcggt tgagtaagga
      901 atatggctta ggtgggagag tcatttatgt gcacgatctc ccgatgccgg gactattacg
      961 ccatgcaaaa gcggtggtga caattaacag tacggtggga atctctgcac tgattcataa
     1021 caaaccactt aaagtgatgg gcaatgccct ctacgacatc aagggcttga catatcaagg
     1081 gcatttacac cagttctggc aggctgattt taaaccagat atgaaactgt ttaagaagtt
     1141 tcgtg
//
LOCUS       DAZUCE010000489         1136 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZUCE010000489 DAZUCE010000000
VERSION     DAZUCE010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.492"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..492
                     /locus_tag="ACHY03_005282"
     CDS             <1..492
                     /locus_tag="ACHY03_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system domain-containing
                     protein"
                     /protein_id="HFS3908359.1"
                     /translation="SWIQQQVMECGERWAEEPSVTITAAPGDNDILSLEPEALQIADN
                     EGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTGKNDLALHLLAELDERATRLTL
                     SQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDMEHLLAGLIAIDAARAAVLCNP
                     GSS"
     gene            494..>1136
                     /locus_tag="ACHY03_005283"
     CDS             494..>1136
                     /locus_tag="ACHY03_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFS3908360.1"
                     /translation="MSVMHYLQDKTFQDIVKNLVLPLLSGIIIPLVKWFVQHYGYAPN
                     IRKYRFEKIPVSEKESILARIDKLTKEPTTKNTLVRIKYCYEQMGIYLPIWCCNKLIC
                     FISDRNVSSVDNRLHCFLKYSFVGMFSDGKFTVNTRRVHKGYRMIAVFAVFSLCVQFT
                     GGIFTTMPFLSGGNTVLFMLFSLVYFIMIFLTVIFTCNLINEIRLAVQFGRLFE"
BASE COUNT          296 a          223 c          282 g          335 t
ORIGIN      
        1 agctggatac agcagcaggt gatggagtgt ggtgaacgct gggcggaaga accgtctgta
       61 acgataacgg cagcccccgg agataacgac attttatctc ttgagccgga agccctgcag
      121 attgcggata atgaaggaac agaagccgct ttaagctggt tacaggcacg tcccggtatt
      181 cagtctgacc gcagcaactg gctactgcgc ctgttgatgg cccgtgttgc cgaacagact
      241 ggaaaaaatg accttgccct gcatctgctt gcggagctgg atgaacgtgc aacccggctg
      301 accctgtcac aatgggaacc tgaactggta tttgaggtaa aagcccgccg cctgaaactg
      361 ttacgtatga agtccgcaaa aactgaaagt gacagggtgc gtctgcagcc tgatatggaa
      421 catcttcttg ccgggctcat cgccattgat gctgccagag ctgctgtgtt gtgcaatccg
      481 gggagctcat gagatgtctg tcatgcatta tcttcaggac aaaacatttc aggatatagt
      541 taaaaatctg gtgttaccgc ttctgtcggg cattattatt cctcttgtca aatggtttgt
      601 gcagcattat ggctatgcgc cgaatatcag gaaatacaga tttgagaaaa ttcctgtatc
      661 tgaaaaggag tccattcttg cccgtattga caagctgaca aaagagccaa caacgaaaaa
      721 tacactggta agaataaaat actgttatga acagatgggg atatatcttc cgatatggtg
      781 ttgtaataag ctgatttgtt ttatatccga cagaaatgtc agttcagtgg ataacagact
      841 gcattgtttt cttaaatatt cttttgtcgg tatgttttca gatggcaaat ttactgttaa
      901 tactcgtcgt gtgcataaag gttaccggat gatagcagtg tttgcggtgt tttctttatg
      961 tgttcagttt acgggaggga tatttacaac catgccattt ctgtcggggg gtaatacagt
     1021 tttatttatg ttattttcgc tcgtttattt tatcatgata tttctgacag ttatattcac
     1081 atgtaatctg attaatgaaa tccggcttgc cgttcagttc gggcgattgt ttgagg
//
LOCUS       DAZUCE010000490         1133 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZUCE010000490 DAZUCE010000000
VERSION     DAZUCE010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.493"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(601..>1133)
                     /gene="hdeD"
                     /locus_tag="ACHY03_005284"
     CDS             complement(601..>1133)
                     /gene="hdeD"
                     /locus_tag="ACHY03_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFS3908361.1"
                     /translation="EMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILSTVVGALLI
                     CSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIAAFIAGLFC
                     VAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVSTLVGIELIF
                     SAASLFSFASLFVKQQ"
BASE COUNT          383 a          248 c          226 g          276 t
ORIGIN      
        1 tgttatcagc ttgtaaatag taaacaaaaa gttaacgttt gcggattact gcccaagaat
       61 aagttatcaa taaccagttt gtgatctctg aagaatatta ctaaagttaa aatgtaatcc
      121 gatttaaata tcgagtctcc ttgtttcgac ttaagctggc aattggattg ccagctttct
      181 tttaacgcat tgaaattacc agagtaaata acgcattcat gtcttcaggt ttttatatag
      241 cgtctggaga cacggatatt tatgtaatgt aatcaacgcc agttgcaaaa aaatgaaccc
      301 ggattactta tttaaccgcc catagcatca acgcttacgg aactctttgt tcactttccc
      361 acagcacttt taaaacatta aacctgacta cggaaaatat cagccatgat aatgtttgaa
      421 atggctaatt tgccatggag tgaaaaaaat tagatgaaat tcagtaggtt gaaataatca
      481 ctagcaggta attatttcaa tgatagtgcg caattgatct acaacactgc gtagcggaga
      541 gagtattaat cggatcatag tcacatcaag tgactatgat ccgagtgaca accgggataa
      601 ttattgctgc ttaacgaaca aactggcgaa gctgaacagg ctggcggcgc taaatatcag
      661 ttcaattccc accagtgtgg aaaccagcgt tacagacacc atcggcgttg caccaaggaa
      721 tatccaggca atgacgatat ccagcacacc aataacgagc tgtagccagc tgcctttcat
      781 tgaacgctga cgataccaac tcatcaggcg aataacccct gcaacacaga acaaaccggc
      841 aataaatgcc gcaatggcaa aaatgcccag ctccggtgcg cggatgaaga aatagccgat
      901 caataaatag gcgactgcga cgaggaaacc ggataatacc ggccagaaat tatgactgcg
      961 gttgctgaat aacccgacaa taagcgcaat acccgagcag attaataatg cacccactac
     1021 tgtgcttaaa atatcgccag agacgaacgg gaaactgata cacagcaacc cgacgataaa
     1081 cagcagcacg gcaataaact ggattgctct gcgatgcttt ttcagcatct cca
//
LOCUS       DAZUCE010000491         1130 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZUCE010000491 DAZUCE010000000
VERSION     DAZUCE010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.494"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..517
                     /gene="atoD"
                     /locus_tag="ACHY03_005285"
     CDS             <1..517
                     /gene="atoD"
                     /locus_tag="ACHY03_005285"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFS3908362.1"
                     /translation="IANDTAFVDTGIGPLIVNGQVRKVIASHIGTNPETGRRMISGEM
                     DVVLVPQGTLIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADL
                     ALIRAHRCDTLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAV
                     IDHIIVSQESK"
     gene            517..>1130
                     /gene="atoA"
                     /locus_tag="ACHY03_005286"
     CDS             517..>1130
                     /gene="atoA"
                     /locus_tag="ACHY03_005286"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFS3908363.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGARKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTETRFEV"
BASE COUNT          260 a          302 c          314 g          254 t
ORIGIN      
        1 gatagccaat gataccgcgt ttgttgatac tggcatcggt ccgctcatcg tcaatggtca
       61 agtccgcaaa gtgattgctt cacatatcgg caccaacccg gaaacaggtc ggcgcatgat
      121 atctggtgag atggacgtcg ttctggtgcc gcaaggtacg ctaatcgagc aaattcgctg
      181 tggtggagct ggacttggtg gttttctcac cccaacgggt gtcggcaccg tcgtagagga
      241 aggcaaacag acactgacac tcgacggtaa aacctggctg ctcgaacgcc cactgcgcgc
      301 cgacctggcg ctaattcgcg ctcatcgttg cgacacactt ggcaacctga cctatcaact
      361 tagcgcccgc aactttaacc ccctgatagc ccttgcggct gatatcacgc tggtagagcc
      421 agatgaactg gtcgaaaccg gcgagctgca acctgaccat attgtcaccc ctggtgccgt
      481 tatcgaccac atcatagttt cacaggagag caaataatgg atgcgaaaca acgtattgcg
      541 cgccgtgtgg cgcaagagct tcgtgatggt gacatcgtta acttagggat cggtttaccc
      601 acaatggtcg ccaattattt accggagggt attcatatca ctctgcaatc ggaaaacggc
      661 ttcctcggtt taggcccggt cacgacagcg catccagatc tggtgaacgc tggcggacaa
      721 ccgtgcggca ttttacccgg cgcagccatg tttgatagcg ccatgtcatt tgcgctaatc
      781 cgtggcggtc atattgatgc ctgcgtgctc ggcggtttgc aggtagacga agaagccaac
      841 ctcgcgaact gggtagtgcc tggaaaaatg gtgcccggta tggggggcgc gatggatctg
      901 gtgaccgggg cacgcaaagt gatcattgcc atggaacatt gcgccaaaga tggttcagca
      961 aaaattttgc gccgctgcac catgccgctc actgcgcaac acgcggtgca tatgctggtc
     1021 actgaactgg cagtctttcg tttcattgac ggcaaaatgt ggctcaccga aattgccgac
     1081 gggtgtgatt tagccaccgt gcgtgccaaa accgaaactc ggtttgaagt
//
LOCUS       DAZUCE010000492         1128 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZUCE010000492 DAZUCE010000000
VERSION     DAZUCE010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.495"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..447
                     /locus_tag="ACHY03_005287"
     CDS             <1..447
                     /locus_tag="ACHY03_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS3908364.1"
                     /translation="IHRFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVRED
                     DWWRNGQNLYLDNLAENGFYRVSPSRAQAGDILLCCFGASVPNHAAIYCGNGELLHHI
                     PEQLSKRERYSEKWQRRTHSVWRHRHWSASAFTGIYNDLVVASACM"
     gene            444..1025
                     /locus_tag="ACHY03_005288"
     CDS             444..1025
                     /locus_tag="ACHY03_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS3908365.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRAVIHIVPRLAGAGGNGVFQVVLGAAAIVGSFFTAGATMALWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          245 a          276 c          359 g          248 t
ORIGIN      
        1 attcaccggt tccgctgtgt gccgcacctg accggacggc gctttgaaca cggtgtgacg
       61 gactgttaca ccctgttccg ggatgcatac catctggcgg ggataacgct gccggatttt
      121 gtgcgtgagg atgactggtg gcgcaacggt cagaacctgt acctggacaa cctggcggaa
      181 aacggctttt accgggtgtc tccgtcccgt gcacaggcag gcgatattct gctgtgctgc
      241 tttggcgcat cggtgccgaa tcatgccgcc atttactgtg gcaacggtga actgcttcac
      301 catatacctg aacaactgag taaacgggag aggtattctg aaaaatggca acgacgaacg
      361 cattctgtct ggcgtcaccg ccactggtcc gcatctgcct tcacggggat ttacaacgat
      421 ttggtcgtcg catcagcctg tatgtgaaca cggcagcgga ggccatccgt gccctgtcgc
      481 tgcaggtgcc gggattccgc tgtcagatga acgaaggctg gtatcagata cgtattgccg
      541 gtgaggatac cgcgccggag gcggtgtatg cccgtcttca tgagccactg agcgggaggg
      601 ccgtgattca tattgtaccg aggctggcag gggccggggg aaatggtgtt tttcaggtgg
      661 tgctgggggc tgcagccatc gtgggttctt tcttcaccgc cggtgcaacg atggcgttgt
      721 ggggcgcagc cctgagtgcc ggagggctga ctgccaccac aatgctgttc tcactggggg
      781 ccagcatgat actgggtggt gtggcccaga tgctggcccc gaaggcaaag acgccggaat
      841 acaaaagtac ggataacggt aaacagaaca cgtatttttc gtcactggac aacatgattg
      901 ctcagggtaa cccgatgccg gtgccttacg gtgaaatgct ggttggttca cgacggatat
      961 cccaggacat cagcacccgt gatgagggcg gtgacgggaa agtggtggtt atcgggcggg
     1021 gatgaaaata aaaaaatccc gcagagttag cggagctgcg ggagagaacg atgaagatta
     1081 actttatgga gttatttttc aggcatcgaa aaagtaacgc agcgtcat
//
LOCUS       DAZUCE010000493         1121 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZUCE010000493 DAZUCE010000000
VERSION     DAZUCE010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.496"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..557)
                     /locus_tag="ACHY03_005289"
     CDS             complement(<1..557)
                     /locus_tag="ACHY03_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFS3908366.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGKSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTIRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTEQRFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNASNDPQLRHIDKILVNKNR"
     gene            815..>1121
                     /locus_tag="ACHY03_005290"
     CDS             815..>1121
                     /locus_tag="ACHY03_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFS3908367.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLISNLHTQNVYSTQEPLTHDEID
                     "
BASE COUNT          382 a          208 c          160 g          371 t
ORIGIN      
        1 ttcctgtttt tgtttacaag aattttatcg atatgtctca gctgcggatc attactggca
       61 ttaacaatat aaaaagcaat gaaaatgata gcaaaaagaa aaaaaagcat tacatactta
      121 aacagcttac tgctaaagaa acgttgttct gtagagataa tgttattttc aataatgcct
      181 ggcaatgaat actgccaaac ttcatttttt tcttttacag caaatttgag acctaaaact
      241 tctatttgct gattcaatgt taacgttcta tcttcaggtt cttgtgctgt taattctctg
      301 attgttacaa ccagatcagg gtcggtagta attattatct caaaactacc tgtaagctcg
      361 ccataaggaa cgactattgt attttctggt aactcagaaa aagcatcact ttttcctaac
      421 gaaacatcat taccaatgat aacaagtaga cgtccattaa gtatttcata ctcgcatcca
      481 ttcaatggtc cgctcaacaa acgaatggca taagattcca ataagtcttg tgataagaat
      541 ttatcattat tttccatgaa taccacccgc taattccata agctctagtt tagagtagtg
      601 taacaagacg gaaatttcat gaccaaatta tgatgacaca gagtaaaaaa attataattt
      661 cgaggactat tttaaatgcc tctatgcata actttattta caatttaagg tataaaccct
      721 attccctcaa tgttaagcaa atataacaat tactcttatt ttaactttta cacgtaatat
      781 ttaccctaaa tgattttaga gttcaggttg gcatatgcaa gtcttttcca gtgatgtcta
      841 cttcactgta ggcactaatg cattattagc atcccaaaaa gaatattaca gcgatttagt
      901 tgcgttggtt gatcttgggc attcgttcgt tgttattgat gagcatcaac atcgaaatct
      961 caaaccaaat acagaacctg taaacatcct tttaagcaac aatttcatca ggatcaataa
     1021 gaatattaca ttatctgatt taacgcattt cctgatttcg aatcttcata cacaaaatgt
     1081 gtattccaca caagaaccac ttacccatga tgagatagat a
//
LOCUS       DAZUCE010000494         1117 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZUCE010000494 DAZUCE010000000
VERSION     DAZUCE010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.497"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..827)
                     /gene="tdcA"
                     /locus_tag="ACHY03_005291"
     CDS             complement(<1..827)
                     /gene="tdcA"
                     /locus_tag="ACHY03_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFS3908368.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     "
BASE COUNT          359 a          241 c          210 g          307 t
ORIGIN      
        1 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
       61 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
      121 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
      181 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tggtttgtgg caacacccac
      241 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
      301 accagtacaa actcggactc aaacagcggc tcaacatgta aatcctgaag cttcatttct
      361 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
      421 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
      481 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
      541 accgcctcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
      601 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
      661 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggct
      721 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
      781 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
      841 aattacctca ttgacggcat gaagtgtatc aaaatgaaat gaacaggata tgtgcgacca
      901 ctcacaaatt aactttcaat actttccaga gtatcgtcat gtcaattaaa tacctttaaa
      961 tatcaacaag ttaaagtata aaaatcagca taaaccctga tttttgtcaa aaaaaatgac
     1021 ggggataacc atattttatg gcgataacat cattcgttat gtggttaata caaaaaagag
     1081 gctgagacca tatttcaagc aattaccgga taattac
//
LOCUS       DAZUCE010000495         1117 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZUCE010000495 DAZUCE010000000
VERSION     DAZUCE010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.498"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..1059
                     /gene="ireA"
                     /locus_tag="ACHY03_005292"
     CDS             <1..1059
                     /gene="ireA"
                     /locus_tag="ACHY03_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFS3908369.1"
                     /translation="RKPEITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKT
                     TETQSVSVKQKAVFIENEYAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLT
                     LKGGIAKAFRAPSIREVSPGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGF
                     SASATLFNTDFKNKLTSYDIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWR
                     VSANYTFTDSRRESDDESLNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYT
                     GKQIWAAQRNGAKVPRVRNGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDG
                     NWQVDEGRRYWANVRISF"
BASE COUNT          353 a          225 c          261 g          278 t
ORIGIN      
        1 agaaaaccgg aaataaccaa cacgatcata gatgcaaaag tgacggcatt tctgccggaa
       61 aatgtgctga ccatcggagg tcaatttcag catgcagaac tccgtgatga ctcagccaca
      121 ggtaaaaaaa cgacagaaac acagtctgtt tcagttaaac agaaagctgt ttttatagaa
      181 aatgaatatg ctgcaacgga ttctctcgcc ctgactggag ggctgcgcct cgataaccat
      241 gaaatctatg gcagttactg gaatccaaga ttgtatgctg tttataacct gaccgataat
      301 ctcactctca aaggagggat cgcaaaagca tttcgggccc cttcgattcg tgaggtcagt
      361 cctggatttg gaacactgac gcagggtggt gcctctatta tgtatggaaa cagggacctg
      421 aaaccggaga ccagtgtaac cgaagagatc ggtattattt ataataatga cagtggtttt
      481 tcagcaagcg cgactctgtt taatacagat tttaaaaata agctgaccag ttacgatata
      541 ggcacaaaag atccagtcac tgggctaaac acttttattt atgataatgt gggtgaggca
      601 aatatcagag gtgtggagct tgcaactcag attcctgtgt atgataaatg gcgtgtatct
      661 gcaaactata cattcactga ctcccgtcga gaaagcgatg acgaaagtct caatggcaag
      721 tcgctgaaag gggaacctct ggaaagaact cccagacatg cagccaatgc aaaactggaa
      781 tgggattaca ctcaggatat tacattttat tcatctctga attatacagg aaaacaaatc
      841 tgggcagcac aaagaaatgg tgccaaggtt ccccgtgttc gtaatggatt cacttctatg
      901 gatattggta tgaattacca gttcctgcca ggcacgctga ttaattttgc cgtacttaac
      961 gtcacagaca gaaagagcga agatatcgat accattgatg gtaactggca ggtcgatgaa
     1021 ggacgtcgtt actgggctaa tgtaagaata tccttctgag agaatataga taacctgagg
     1081 ccgtgcgcct caggtaccag tatagtaatc agtaacg
//
LOCUS       DAZUCE010000496         1115 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZUCE010000496 DAZUCE010000000
VERSION     DAZUCE010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.499"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..502
                     /gene="atoD"
                     /locus_tag="ACHY03_005293"
     CDS             <1..502
                     /gene="atoD"
                     /locus_tag="ACHY03_005293"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFS3908370.1"
                     /translation="AFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLV
                     PQGTLIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRA
                     HRCDTLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHII
                     VSQESK"
     gene            502..>1115
                     /gene="atoA"
                     /locus_tag="ACHY03_005294"
     CDS             502..>1115
                     /gene="atoA"
                     /locus_tag="ACHY03_005294"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFS3908371.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVCAKTEARFEV"
BASE COUNT          251 a          295 c          312 g          257 t
ORIGIN      
        1 cgcgtttgtt gataccggca tcggtccgct catcgtcaat ggtcgagtcc gcaaagtgat
       61 tgcttcacat atcggcacca acccggaaac aggtcggcgc atgatatctg gtgagatgga
      121 cgtcgttctg gtgccgcaag gtacgctaat cgagcaaatt cgctgtggtg gagctggact
      181 tggaggtttt ctcaccccaa cgggtgtcgg caccgtcgta gaggaaggca aacagacact
      241 aacactcgac ggtaaaacct ggctgctcga acgcccgctg cgcgccgacc tggcgctaat
      301 tcgcgctcat cgttgcgaca cacttggcaa cctgacctat caacttagcg cccgcaactt
      361 taaccccctg atagcccttg cggctgatat cacgctggta gagccagatg aactggtcga
      421 aaccggcgag ctgcaacctg accatattgt cacccctggt gccgttatcg accacatcat
      481 cgtttcacag gagagcaaat aatggatgcg aaacaacgta ttgcgcgccg tgtggcgcaa
      541 gagcttcgtg atggtgacat cgttaactta gggatcggtt tacccacaat ggtcgccaat
      601 tatttaccgg agggtattca tatcactctg caatcggaaa acggcttcct cggtttaggc
      661 ccggtcacga cagcgcatcc agatctggtg aacgctggcg gacaaccgtg cggtgtttta
      721 cccggtgcag ccatgtttga tagcgccatg tcatttgcgc taatccgcgg cggtcatatt
      781 gatgcctgcg tgctcggcgg tttgcaagta gacgaagaag caaacctcgc gaactgggtt
      841 gtgcctggga aaatggtgcc cggtatgggt ggcgcgatgg atctggtgac cgggtcgcgc
      901 aaagtgatca tcgccatgga acattgcgcc aaagatggtt cagcaaaaat tttgcgccgc
      961 tgcaccatgc cactcactgc gcaacatgcg gtgcatatgc tggttactga actggctgtc
     1021 tttcgtttta ttgacggcaa aatgtggctc accgaaattg ccgacgggtg tgatttagcc
     1081 accgtgtgtg ccaaaacaga agctcggttt gaagt
//
LOCUS       DAZUCE010000497         1109 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZUCE010000497 DAZUCE010000000
VERSION     DAZUCE010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.500"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..513
                     /locus_tag="ACHY03_005295"
     CDS             <1..513
                     /locus_tag="ACHY03_005295"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908372.1"
                     /translation="RIEGMKRKSLSEIDLQAAQRLKEIWTAKKNQLGLTQERAAEILG
                     FSTQGAVSHYLNGQTPLNLEAVIKFAGLLQVPPESIRPDMAELLQIVRMYPQESGEDN
                     VVTISADMEQSENELPFNIDPMERDLLQTFRAFPKEDKEKMLKEMKEKKESIEEIVAR
                     WLAAQKGRRA"
     gene            531..722
                     /locus_tag="ACHY03_005296"
     CDS             531..722
                     /locus_tag="ACHY03_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFS3908373.1"
                     /translation="MSTALSPIISEFETVEQENSYNEWLRAKVAASLSDPRPAIPHDE
                     VMAEMENLIAQLAATNRSE"
     gene            722..1012
                     /locus_tag="ACHY03_005297"
     CDS             722..1012
                     /locus_tag="ACHY03_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFS3908374.1"
                     /translation="MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLT
                     EHPYLYPPSERVPSLREIVTHPNYIILYRVTASNIEIVNVVHSRRQYPNKTC"
BASE COUNT          358 a          243 c          238 g          270 t
ORIGIN      
        1 agaattgaag gtatgaaaag aaaatccctg tcagagatcg acctgcaagc cgcccagaga
       61 ctgaaagaaa tctggacggc gaaaaaaaat caactagggt taacccaaga gcgtgcggca
      121 gaaattctgg gattttcgac acagggagct gtaagccatt atctaaatgg tcagacacct
      181 ttaaatcttg aggctgttat caagttcgca gggttgctgc aagttcctcc cgagtcaatc
      241 agaccagata tggccgagtt gttacaaatt gtaaggatgt atccccaaga atctggggag
      301 gacaatgttg tcactatatc tgcagatatg gaacaatcgg aaaacgaact tccgtttaat
      361 atagacccca tggagcggga tttgctccag acgttcaggg ctttccccaa agaagataaa
      421 gagaaaatgc ttaaggaaat gaaggagaaa aaagaatcaa ttgaagaaat cgttgcgcga
      481 tggctagccg cgcaaaaggg tcgtcgcgcc taatctgagg aggtcaaaac atgagtacag
      541 ccctttcccc gataatttct gaatttgaaa cagtcgaaca agaaaacagc tataacgaat
      601 ggttgcgagc caaagtggca gcaagcctct cagatccccg tcctgcaatt ccacatgacg
      661 aagtaatggc tgaaatggaa aaccttattg ctcaattagc tgcaacgaac aggagtgagt
      721 aatgctgccc attttatggc taccttctgc acgtgatgat ttacgtcaga tcgtagccta
      781 tattgctaag gaaaaccctc ccgctgcacg tagactaaaa atacgcattg aaacatcagt
      841 tttgtcactt actgaacacc cttatctgta cccaccgagc gaaagagttc caagtcttcg
      901 tgagatagtg actcatccta actacataat actttaccga gtaacagcat ctaacatcga
      961 gatcgtaaat gtagttcact cacgaagaca gtatccaaac aaaacctgtt aatccttcct
     1021 gtcaacaacc accttcgggt ggtttttttc ttgccacgat aatagcactg ctatttacat
     1081 aattaaatag tagtggtatt gttcattca
//
LOCUS       DAZUCE010000498         1098 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZUCE010000498 DAZUCE010000000
VERSION     DAZUCE010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.501"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            91..330
                     /locus_tag="ACHY03_005298"
     CDS             91..330
                     /locus_tag="ACHY03_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HFS3908375.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            350..682
                     /gene="sctI"
                     /locus_tag="ACHY03_005299"
     CDS             350..682
                     /gene="sctI"
                     /locus_tag="ACHY03_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFS3908376.1"
                     /translation="MSVSNMPPIDRVEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            679..>1098
                     /gene="sctJ"
                     /locus_tag="ACHY03_005300"
     CDS             679..>1098
                     /gene="sctJ"
                     /locus_tag="ACHY03_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFS3908377.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKT"
BASE COUNT          353 a          194 c          237 g          314 t
ORIGIN      
        1 acaaataata gttcatttag ctatggagat aaccgattcg agaagtctca aggtagcaag
       61 tggacgtttc aggaataatt gggaggactt atggcagatt ggaatggtta tattatggat
      121 atcagcaaac aatttgatca gggcgttgat gatctgaacc agcaagttga aaaagcgttg
      181 gaggatttag caaccaatcc ctccgacccg aaattccttg ccgaatatca aagtgcatta
      241 gctgagtata cattgtatcg aaatgcgcaa tctaacgttg taaaagcgta taaagatctt
      301 gattcggcaa tcatacaaaa cttccgctaa actggataag gattttatta tgtcagtaag
      361 caatatgcca cctattgata gagttgagca atcaactgct catgaaatac agcaagcgaa
      421 agtgattgat ttaaatgacc gggttctcaa tcttgacaac ccggatgata aaatgatctc
      481 cgcttttgcc aactatgctg tacaaactga aaattggcag caaaatgcgt tacaagcatt
      541 aaggtctgat aaaaaagggt taactcctga aaaattatta gtcttgcagg atcatgtgct
      601 taattataat gtcgaggttt cattggtggg aacattagca agaaagattg tcgctgcagt
      661 tgagacgttg acccgctcat gaaatatata tcattattat tgtttatcct gctgttgtgc
      721 ggctgcaagc agcaggaatt actgaaccat cttgatcaac agcaagctaa tgatgtcctt
      781 gctgttttac aaagacataa tattaatgct gaaaagaagg atcaaggcaa aactggattt
      841 tcaatatttg tcgaaccaac ggattttgct tctgcggtgg attggctaaa aatctacaat
      901 ctcccgggta agcctgatat tcaaatatcg cagatgttcc ctgcagatgc actcgtttca
      961 tctccacgag cggaaaaagc aaggctatat tctgctatag agcagcggtt agagcaatct
     1021 ctcaaaatta tggatggcat tgtttcttcg agagtacatg ttagttacga tgttgatact
     1081 ggcgatagtg gtaaaact
//
LOCUS       DAZUCE010000499         1075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZUCE010000499 DAZUCE010000000
VERSION     DAZUCE010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.502"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            59..880
                     /locus_tag="ACHY03_005301"
     CDS             59..880
                     /locus_tag="ACHY03_005301"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFS3908378.1"
                     /translation="MNKIYALKYSSLTGGLIAVSELSKKVTGKTGRRLMTVSLVLSVT
                     LSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEIKDNNGNAVGTLDVPMIDFS
                     SVSRRGSLTLLSQGYGVSAKHGDLGDVNNASFGYDKNNYTVVKNNKHSGLDFSLHRFS
                     KLIAEATPADINISGQLSDSSQYTAFYRAGAGTQYIKERSGKQTHIPGTFLTGGTVGT
                     PWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSGSPLFAYDSLSEKMVSGWSHPA"
BASE COUNT          339 a          203 c          237 g          296 t
ORIGIN      
        1 cactctatcc ggacagacaa tacattttaa taccatataa ttatatagca ggaattatat
       61 gaataaaata tacgcgctta aatatagctc ccttactggt gggcttatag ctgtgtcaga
      121 attaagtaag aaggtcacag gaaaaaccgg cagaagatta atgacggttt ccctggtatt
      181 atcagtgact ctttctgctt taccgggtaa agcatcaacg gtcagtgcag aaataccata
      241 tcagactttt cgtgactttg ctgaaaataa aggtgtgttt acccccggag tgacaggaat
      301 tgaaataaaa gataataatg gaaatgcagt tggtacgctt gatgtgccaa tgattgactt
      361 cagcagcgtg tcacggcggg gctcactgac cctgttgtcg cagggatatg gtgtatcagc
      421 aaaacacgga gaccttggtg atgtaaacaa tgccagcttt ggctatgata aaaataatta
      481 tactgtagta aagaacaaca aacattcggg ccttgatttt tctctgcatc ggttcagcaa
      541 actcatcgca gaggctacac ctgctgatat aaatatatct ggacaactta gtgattcgtc
      601 acagtatact gctttttaca gagcaggcgc tggcacacag tatattaaag aacgctcagg
      661 taaacagact catatcccgg gaacattttt aactggtgga acagtaggca ctccatggta
      721 ttcaggaaat aatttaatat caagctctcc aggtgacacc tataataaat cacagggacc
      781 attagccagt tatgggcaga tgggtgacag cgggtcgcca ttatttgctt atgattctct
      841 cagtgaaaaa atggtctctg gctggagtca ccctgcataa ctatggggtg aacggttaca
      901 gaaataactg gttgttatta cctgaggatt atatcagaaa tattattgta gccgatttcg
      961 atcccattat ttcatttaat aaaaacagta aagaacatat gtcatggaca tatgatgcag
     1021 ccaaaggcgt gggccgtatt cagcaggatg accaacagtt tgtgatgcat ggtaa
//
LOCUS       DAZUCE010000500         1071 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZUCE010000500 DAZUCE010000000
VERSION     DAZUCE010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.503"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(133..363)
                     /gene="ynfN"
                     /locus_tag="ACHY03_005302"
     CDS             complement(133..363)
                     /gene="ynfN"
                     /locus_tag="ACHY03_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFS3908379.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(368..433)
                     /gene="ynfR"
                     /locus_tag="ACHY03_005303"
     CDS             complement(368..433)
                     /gene="ynfR"
                     /locus_tag="ACHY03_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFS3908380.1"
                     /translation="MKAPSGAFWLGVYSMDTHILR"
     gene            complement(436..624)
                     /gene="ynfQ"
                     /locus_tag="ACHY03_005304"
     CDS             complement(436..624)
                     /gene="ynfQ"
                     /locus_tag="ACHY03_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFS3908381.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(635..847)
                     /gene="cspI"
                     /locus_tag="ACHY03_005305"
     CDS             complement(635..847)
                     /gene="cspI"
                     /locus_tag="ACHY03_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFS3908382.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          376 a          210 c          185 g          300 t
ORIGIN      
        1 agtaagtcga ttatatggtg caaacgtgta aaagataaga tgaaacatcg caataatcaa
       61 catacgatag tctaaatttt acacaaacag acaaagagaa ttttcctgaa ttatcaatgc
      121 aatagcatca aatcaactca agagccttat tgctgcttcc agaatttctt ctgaagtaac
      181 atgtcgatcc gcggctacat aaatgacttt atgatctccg gtcagagatg gaaaccctgc
      241 ggccattaca gtaaggtgtg ttttttcgcc atttggatat tcacgcatga tggtgttaac
      301 tccagtcatc gctggcacta ccactgctgg ttcagagtta aaaaaaacta tgattttttt
      361 catgatgtta ccgtagtatg tgagtatcca tcgaatagac accaagccaa aaagctcccg
      421 aaggagcctt cattttcact tttttaaatc caacgacaga cggctggcat ttaagtattg
      481 tgaaatatta tcaaatgtaa tcatcattga tttacaaaag atacattttg ccccgaaagg
      541 attcatgtca gaaacatcaa aagatgatgt tctatactgg gaaccatgac aacacgggca
      601 tctaaagtga atatggtttg taatattgtc tacctcaaag cgccactaca tgaacagcgg
      661 caggaccttt aggtccgttc tcaataccaa attcaacttc ctgattctca gttaatgttt
      721 tgaaatcgtt gctctgaatt gctgagaaat ggacaaacac atctttgctg ccatctttcg
      781 gcgtgatgaa accaaaacct ttttcagggt taaaccattt cactaaacca gtcattttgt
      841 tagacataat tattaccttt tgaagaaatt agcccttggg cagaatggtc cgaaaaaaaa
      901 tatcagagag aaaaaccaac aaggaaatct caagaggtac aaataataaa attataacaa
      961 tgactgcttc agataaattt gtaacaaacc agaacaccat taacgcatga ttaaccaccc
     1021 atagcaagga ttacttttgt aaagaaaaac acagcaatga aagaatagct t
//
LOCUS       DAZUCE010000501         1063 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZUCE010000501 DAZUCE010000000
VERSION     DAZUCE010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.504"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            151..>1063
                     /locus_tag="ACHY03_005306"
     CDS             151..>1063
                     /locus_tag="ACHY03_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFS3908383.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRVGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSVSGASLEVDNGQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGGNEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGTQAAGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     ANSNGKVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGAVN"
BASE COUNT          293 a          190 c          279 g          301 t
ORIGIN      
        1 ttcgaaagca ttagcaaatt agccaaattg acaccttccc acctccaact aacactcaat
       61 acagacctct gacaatttaa taagtttcat ttttaatgtc tggcgactca tctctttcgt
      121 ggctgtattg tttgtatatg gagcgtttat atgaatagaa tctatcgcgt gatatggaat
      181 tgcactctac aggtatttca ggcctgctcg gaattaactc gcagggtagg taaaacatca
      241 acggttaatt tgcgaaaatc ctctggactg acaacgaaat tcagtagatt gacgctgggt
      301 gttttgctgg cactaagcgg ttcagtgtct ggtgcgagtc tggaagttga taatggtcag
      361 attaccaata ttgatactga tgttgcttat gatgcctacc tggttggctg gtatggcact
      421 ggagtgctta atattttggc tggcggtaat gcctccttaa ccactattac taccagcgtc
      481 attggcggta atgaggattc ggagggtacc gttaatgttt tgggtggcac ctggcgattg
      541 tatgatagcg gaaataatgc aaggccttta aatgtgggtc aatccggaac ggggacgctg
      601 aatattaaac agaagggtca cgtcgatgga ggctatttaa gattaggtac tcaagctgca
      661 ggcgtcggga cggttaatgt tgaaggagag gactcggttt tgacgaccga attattcgaa
      721 atagggagtt acggtacggg ctcattaaat attacggata agggttacgt cacgagttca
      781 atagtcgcca ttttaggata tcaagcgaac agtaatggta aggttgtcgt tgaaaagggt
      841 ggcgagtggc taataaaaaa taatgattcc tcaattgaat ttcaaattgg taatcaagga
      901 actggggagg cgactattcg cgagggtggg ctgattacgg ctgaaaatac gattatcggt
      961 ggcaatgcca ccggtgtcgg aaccctgaat gtgcaggatc aagactctgt catcacggta
     1021 cgcagactct ataatggata tttcggtaat ggcgcagtca ata
//
LOCUS       DAZUCE010000502         1020 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZUCE010000502 DAZUCE010000000
VERSION     DAZUCE010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.505"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(740..>1020)
                     /locus_tag="ACHY03_005307"
     CDS             complement(740..>1020)
                     /locus_tag="ACHY03_005307"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908384.1"
                     /translation="VTSKINIKNSAKFNIRSTSRLTYDIISQLHLPQNGYFMEHSNFL
                     VAIIKLILRSLAIIPGWFVSLSHEIVTFLFILEIWGNAGDDFCIYIRW"
BASE COUNT          306 a          191 c          201 g          322 t
ORIGIN      
        1 ggtctcctgt gaaaaatctc ttttcacatt atttaaataa acagagagcc agaataaata
       61 cctgagtata aaatctctgc tgatgtttaa ttgatttgaa ttttcgtaag ctatatcact
      121 tactcaatcc attttaccca gagtcaaaaa atagactgtc tgttgatctc attttcatat
      181 tcctttcaga ctgaagtgcg ttttcatcac ccgatgagaa agaactgaag cgatgtttgc
      241 catagattgc aagatgccct gtctcattgt ctatcgtggt gcacctttat acctgttata
      301 ccagatcaaa aatcacgcaa tccatacaac aaaaccagat ttgcaattcg tgtcacaaaa
      361 tatgtcgatc tttttctaag aagaagatgc catgtgaagc cagacgaaca cttgcggtgg
      421 tcttcaaaaa ctaaagatct tcttttaact atatgtttta taaataattt atttagagtc
      481 taaacaaggg aagctttgca agctaactca gtgggcgtgg tgaaaataag tgtttacccg
      541 ccatcaggct gagcataatt ctcatcatga aatatgtttc ctggtttgtg gcttgtaact
      601 ggtcactcct gaagtcgatc tggcgaggct tgttgatgtt ggtgttttca gggtgatgcc
      661 tcacttagtt tgtttgccgt atcgcccggc gaatggctgt gattgaggaa ggtttaagtc
      721 gcagtgacca aagccatatt taccagcgaa tgtagatgca aaaatcgtct cctgcgttcc
      781 cccatatctc caggataaaa aggaatgtaa caatctcatg gcttaaactg acgaaccagc
      841 caggaataat cgctagggac ctaagaatta gcttgataat agccactaag aaattactgt
      901 gttccatgaa atagccattt tgtggcaaat ggagttgact aataatgtca tatgtgagac
      961 ggctagttga acgaatatta aattttgctg aattttttat gttgatttta cttgttacag
//
LOCUS       DAZUCE010000503         1010 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZUCE010000503 DAZUCE010000000
VERSION     DAZUCE010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.506"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..486
                     /gene="lpfA1"
                     /locus_tag="ACHY03_005308"
     CDS             <1..486
                     /gene="lpfA1"
                     /locus_tag="ACHY03_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HFS3908385.1"
                     /translation="TSGTVFAAESGDGTVKFTGEIVDSPCVLSVDSQNQEVVLGQVQK
                     SVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFTGVGDADKSDLISVSTEAGAAKGV
                     GIGIYDNSNTLVALNGGKASVDLKTGQTVLYFTANYVSTLATVTTGYGNAQVDFNLSY
                     E"
     gene            551..>1010
                     /locus_tag="ACHY03_005309"
     CDS             551..>1010
                     /locus_tag="ACHY03_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS3908386.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSSTRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFKLDPEKNNILRIVNITPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLKGDVKTAPNELRF"
BASE COUNT          276 a          220 c          244 g          270 t
ORIGIN      
        1 acttctggca ctgtattcgc agcagagtct ggtgatggca cggttaaatt tacaggcgag
       61 atcgtggatt caccttgcgt actgtccgtt gactctcaga accaggaagt tgtgttgggt
      121 caggtacaaa aatctgtatt tgctgcggtt ggtgacaaat ccccggcgaa acctttcgaa
      181 atcaaactgg aagattgcga taccacaacg atgaaaaaag ccaatgtgag ctttactggt
      241 gttggtgatg cagataaatc tgatctgatt tctgttagca ctgaagcagg tgcggccaaa
      301 ggtgttggta tcggtattta tgataacagc aacaccctgg tcgctcttaa cggtggtaaa
      361 gcaagtgttg acctgaaaac aggccagacg gttctctact ttaccgccaa ctacgtttca
      421 actcttgcaa cggtaacgac cggttacggc aatgcgcagg tcgatttcaa cctgtcttac
      481 gagtaatcgt ccgttttaca aatggcggcg taattgccgc cattagtcgt caaagttcat
      541 ggatcgaatg atgaaaagta aatttgttgc gttggctctc tctctgttcc tttcacagtc
      601 agttctggcg ggtggcgtcg gacttagctc tactcgcgta atttatgatg gcagtaagaa
      661 agaagcctcg ctgaccgtac agaataaaag taagacggaa gagtttctta tccaatcctg
      721 ggtggatgat gccgcgggca gcaaaaaaac accgtttatt attacgcccc cactattcaa
      781 gcttgatcca gaaaagaaca atattttgcg tattgtgaat attacgcccg gcctgcctca
      841 ggatcgcgaa tcggtctact gggttaatgt gaaagccatt ccatctaaaa gtgacgacag
      901 cgaaaacaaa aatgttttgc agattgccgt tcgcacccga atcaaacttt tttatcgccc
      961 ggcaggttta aagggtgatg taaaaaccgc gccaaatgaa ctgcgattta
//
LOCUS       DAZUCE010000504         1009 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZUCE010000504 DAZUCE010000000
VERSION     DAZUCE010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.507"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..312
                     /locus_tag="ACHY03_005310"
     CDS             <1..312
                     /locus_tag="ACHY03_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908387.1"
                     /translation="EYISRTGSDFFTVNNEGKWEAASEGIPVLADINITPEFVGMNAI
                     VSCRINDDSGYHVDQCFQAEITFPENHFIIFTGTGDSALFMVYKNIYASLKVKDHVLR
                     R"
     gene            429..>1009
                     /locus_tag="ACHY03_005311"
     CDS             429..>1009
                     /locus_tag="ACHY03_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908388.1"
                     /translation="MNKTSLALLTLILFITTHCANANKKALTIPLDSTKITPLVIFYK
                     NITNGITELFLSETEKSQTIPFNQQEITIPVKGENFLSPWIANDTHYYELGQFEDKDN
                     IFKIIIYNTIGESDTPLLNVQLNSYDRKGILLDALLLSTFFGYEDIIRFSHFKISPDY
                     TIAINNYVIHPYEPSEYGTTPHKKNPKPEVYIRA"
BASE COUNT          352 a          206 c          160 g          291 t
ORIGIN      
        1 gaatacatat cacgtacggg aagtgacttt ttcacagtaa acaatgaagg gaaatgggag
       61 gcggcaagcg aaggcattcc cgttttagct gacataaata ttacgccaga atttgttggc
      121 atgaacgcaa tcgtttcatg ccgtattaat gatgattctg gatatcatgt agatcaatgt
      181 tttcaggcag agataacttt cccagaaaat cattttatta tttttacagg cacaggagat
      241 tcagccttat ttatggttta caagaatata tatgcatcgc ttaaagtaaa agatcacgtt
      301 ctgaggagat agcctcttat gatggggtga ctctcttatt cagctttaac agcttgcata
      361 gaaaaaacaa aaatacaact ttcgaaacac atctgataat tcatttattt tatcgcgatc
      421 gtataaacat gaataaaaca tcactcgcat tactgactct tattcttttt attactaccc
      481 actgtgcgaa tgctaataaa aaagcactaa cgataccatt agactctaca aaaataacac
      541 cacttgtgat tttttataaa aacatcacca acggaattac agaactcttc ttatctgaaa
      601 ccgaaaaatc ccaaacaata cctttcaatc agcaagaaat taccatccct gtcaaaggcg
      661 agaatttcct ctcaccctgg atagctaacg atactcatta ttacgaattg ggtcagttcg
      721 aagataaaga taatatcttc aaaattatta tttataacac cataggtgaa agtgataccc
      781 cattactcaa tgtccaactg aatagctatg acaggaaagg cattctccta gatgccctat
      841 tgctcagcac tttctttggc tacgaagata tcatccgctt ttcacatttt aaaattagcc
      901 cggactatac gatagcgata aataactatg ttatccatcc ttacgaacct agtgaatatg
      961 gaacaacacc gcacaagaag aacccaaaac ctgaagtgta tatacgagc
//
LOCUS       DAZUCE010000505         1008 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZUCE010000505 DAZUCE010000000
VERSION     DAZUCE010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.508"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            167..733
                     /gene="slp"
                     /locus_tag="ACHY03_005312"
     CDS             167..733
                     /gene="slp"
                     /locus_tag="ACHY03_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFS3908389.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            889..>1008
                     /locus_tag="ACHY03_005313"
     CDS             889..>1008
                     /locus_tag="ACHY03_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3908390.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQN"
BASE COUNT          315 a          204 c          213 g          276 t
ORIGIN      
        1 gatggatact catgccacgc cccaaaataa cggaattgaa ctaaacagaa gggatataaa
       61 catcagacag gtttacgtta ctatcaggca tatcgcctca gaatcagatg aaaactataa
      121 agaaatatct attatggttt taatatttgt tgataaggat agtaacatga acatgacaaa
      181 aggtgcactc atactcagcc tttcattttt gcttgccgca tgtagttcaa ttccgcaaaa
      241 tatcaaaggc aataaccaac ctgatattca aaaaagtttt gttgctgttc ataaccagcc
      301 ggggttatat gttggtcaac aagcgcgctt tggtgggaag gttatcaacg ttatcaatgg
      361 caaaacggat acgttgttag aaatcgctgt attaccgttg gatagctatg cgaagcctga
      421 tattgaagcc aactatcagg gccgactgct cgccagacaa agcggcttcc ttgacccagt
      481 gaactatcgt aatcactttg ttaccatcct cggtaccatt cagggtgaac aaccaggctt
      541 tatcaataaa gtcccgtata acttcctgga agtgaatatg cagggcatcc aggtgtggca
      601 tttgagagaa gtggttaata ccacctataa cctgtgggat tacggctatg gtgcattctg
      661 gccggaaccg ggctggggtg cgccttacta caccaatgcg gtgagtcagg taacacctga
      721 gctggtcaaa taacaccacc gaaagatgca gatgctttct cagcatctgc atcatgtatt
      781 acataaaatt aatatacagt aagctaacta ttattattat aagccctgtc ctgttaatta
      841 cctttggcaa actgattata aagttaatgt ccgcaccagg agtcggttat gtttcttata
      901 attaccaggg atacgatgtt cttcaccgcg atgaaaaaca ttctgagtaa aggtaatgtc
      961 gttcatatac agaacgaaga agagatcgac gtaatgttgc atcagaat
//
LOCUS       DAZUCE010000506         1007 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZUCE010000506 DAZUCE010000000
VERSION     DAZUCE010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.509"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            412..>1007
                     /locus_tag="ACHY03_005314"
     CDS             412..>1007
                     /locus_tag="ACHY03_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908391.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDPLKTDEESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNRDEEGHYLAFE
                     YNVDTSDRITVSAYNEKKPGCYGQCLVIQDDETDLFNLIKVKIFEARHNYVAQLMSVP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDIL"
BASE COUNT          322 a          186 c          184 g          315 t
ORIGIN      
        1 ggtgatttta ttgttaattt attgtgaaat atttacgcaa cacaattatt acaatgaaaa
       61 ggtatagata aaaaaccagt attttattta acagaaccgc cttatccact tttataatac
      121 caattgatta gagtagccaa tttgttgcta ttttacataa catattttta tcaaagttaa
      181 ttgattgttg cctttatgag caccggccgt aagcccgtta tgttattgtt tcattagtaa
      241 taatcactta aatgtatcga tgaaacaggt atgacttagc gggagtgatt gtttctgagt
      301 gtacagagac cactttcgcc tgataaatga aaaatgagcg atttttgcaa gttagttttt
      361 ctatagccga gtacgctcag cgcctcgctt gattacaatg aggttgctgc tatgagtcct
      421 gataatatcg gttcggctgt caatcgcttc tcaggttggc catctaatga tcctctaaaa
      481 acagatgaag aatcaatgac atcacccgtg actgtgcagg taataaagat gattgccgcc
      541 atccttgatc cacatcggat agtcgttgga attgatacac tttatacaac gcagcgcgaa
      601 acatttacct cagagagtca acagctcatt gaatcagcat tgcaaacact tcatgtgatg
      661 aatttaaata atctggaaac aaatcgcgat gaagaaggtc actatctggc ttttgaatat
      721 aacgttgaca cctctgacag aataaccgtt tctgcttata atgaaaaaaa gcctggctgc
      781 tatggccaat gtcttgtaat acaggatgac gaaacagact tatttaacct gataaaagtt
      841 aaaatttttg aagccaggca taattatgtc gctcaattga tgagcgttcc ggaatttatg
      901 ttcaaattta gttatagcgc tcaacaaaat agattagaac ctcatcttct ggttcccact
      961 tatttccctc taaaaacaaa cgaaccggtt acgcaagagg atatact
//
LOCUS       DAZUCE010000507          982 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZUCE010000507 DAZUCE010000000
VERSION     DAZUCE010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.510"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..714)
                     /locus_tag="ACHY03_005315"
     CDS             complement(<1..714)
                     /locus_tag="ACHY03_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908392.1"
                     /translation="MLTIDASNNVKICKVSYCEVTMVSVTSYTYPTETENDNSQFQSQ
                     SLRESIDKSLEDNLHKFNMIEQSGLLDNYRARFKDDTFRDIFRSFLLYLLDSFHIYRH
                     DISDKLVPYFHVTPESENASYKTWIAVTGMNSYIKIEQTGSNKFKVTDMYREFNDETK
                     CFDKKCRDMDFSGRNVKEFIRLLSDHDQFEKDQISELTVAANALKLEVAKNNYNMKYS
                     FDRQTERRMIELIREQKDLI"
BASE COUNT          315 a          195 c          129 g          343 t
ORIGIN      
        1 aattaaatct ttttgttccc ttataagctc tatcattcgg cgttcggttt gcctatcaaa
       61 agagtatttc atgttatagt tatttttagc aacttccagc ttaagagcat ttgcagcaac
      121 tgttaattct gagatttgat ctttttcaaa ttgatcgtga tctgataata accttataaa
      181 ctcttttacg tttcgaccac taaaatccat atccctacac tttttatcaa aacactttgt
      241 ttcgtcatta aactcacgat acatatcagt gactttaaat ttattacttc cggtctgctc
      301 aatttttata tagctattca tgcctgtaac ggcaatccat gtcttataac tcgcattctc
      361 cgattcaggc gtaacatgaa aataaggaac caatttatca ctaatatcat gccgatagat
      421 atgaaatgaa tctaaaagat ataataaaaa acttcgaaaa atgtcacgaa aagtgtcatc
      481 tttaaacctt gccctataat tatcgagcag gccgctttgt tctatcatat taaatttatg
      541 aagattatcc tcgagacttt tgtcgatact ctcccttaga ctctgactct gaaactggct
      601 attatcattc tctgtttccg ttggatatgt ataactcgtc acactaacca tagtaacctc
      661 gcaatatgac actttgcata ttttgacatt gttagaagca tcaatggtta gcatgataaa
      721 tatctcaata ctaaccgcta tatttcattt atttcaataa taagactata gtgcagttaa
      781 tttttatcga tttccccaat gcaattgcat cactaacact acttattcac tatcaaaaat
      841 cgctcatttt ttcaaaaaga ctgtttacac acagacggtt aagaatacgc gttcaactta
      901 atgataaacg cgctttatca ggaaaaaatt tataaatcag cagttgttgc ggcaccgaaa
      961 aatttaaaaa cggttttatc gc
//
LOCUS       DAZUCE010000508          982 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZUCE010000508 DAZUCE010000000
VERSION     DAZUCE010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.511"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(497..685)
                     /locus_tag="ACHY03_005316"
     CDS             complement(497..685)
                     /locus_tag="ACHY03_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFS3908393.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            complement(760..>982)
                     /locus_tag="ACHY03_005317"
     CDS             complement(760..>982)
                     /locus_tag="ACHY03_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFS3908394.1"
                     /translation="AFMSVEKLTLSGRQVNVLMMLLYGSNGTEGARYLGMSEKTFSTH
                     KQNLIKRLRVHNEVELLYKGMLLVRKGRL"
BASE COUNT          315 a          261 c          169 g          237 t
ORIGIN      
        1 aattatccat accaactggt aagcgacaca aattaaactt attatcacaa caaacaactc
       61 tgcaaatgag tcaagaaaaa caacagacag aaaaacattc aaagtacaca gcaaaagtat
      121 ctgtagtcgg ggaaaatccc ataccctgac aacccatgat gtattaccgg aaacagggat
      181 aaaagttatg actgccagaa ggatagcagt aaaaataaaa acacaagtta tcacaaatca
      241 ctccttgttc tgaaccggca cacaaaactg tcatatacgt ttcaaaagta aaaacacact
      301 gctgccacaa gattcacgac gtaaccgggc agcatatcct gattacggac aatccatgaa
      361 accgcctcgc cagaagcgtc catcacatcc ggtttttccc tgttttatat tccacgaacc
      421 attttatttt caggaatctc cgggccttta tcccgcatca tatacacagc tatgacaacc
      481 cgctcttctg cagtcatcat ttccccgtgg cttcgcggat tttctgttcc gtcttatact
      541 gcgaaagcgc atacacggcc catattgttg ccggtatcca gcccagcagc gttatctgta
      601 acaccagaca aaaaatcccc gtcagaggcc ggccgatggt gaaaaaagtc agccagggaa
      661 gaagaaaagc aatcaacaga cgcataaaaa ctccttatga ttatgaaatt aacgaatcag
      721 acgctgcgtt taacattacc ggaaataagc tgtcctgctt cacaacctcc cttttctgac
      781 cagaagcatc cccttgtaca acaactcaac ttcattatgt acccgtaatc tctttatcag
      841 attctgctta tgagtggaga atgttttctc tgacatgcca agatatcgtg ccccctctgt
      901 tccgttactg ccataaagca gcatcatcag tacatttacc tgccggcctg acagggtcag
      961 cttctccacc gacatgaatg cg
//
LOCUS       DAZUCE010000509          966 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZUCE010000509 DAZUCE010000000
VERSION     DAZUCE010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.512"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..965)
                     /locus_tag="ACHY03_005318"
     CDS             complement(<1..965)
                     /locus_tag="ACHY03_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFS3908395.1"
                     /translation="MILGLMVMIIAMLIIPLPTYLIDFLIGLNLTLAILVFLGSFYVD
                     RILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSFGEFVIGDSLVVGFVI
                     FSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLRAGIIDADLAKERRSV
                     LERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQNGMDFSTALTVYTIL
                     TVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLSNSFVIIVTCVLALSI
                     GLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVERDITVGLEP"
BASE COUNT          348 a          215 c          160 g          243 t
ORIGIN      
        1 ggttccaacc caacggtaat atctctctca acaaccgttt cttcagtccc tttctttttg
       61 aatttaaagt aaaagtaaat tcctaagatc actgccaaac ataaaaacac aagaagggga
      121 aaacctggaa gtaacccaat acttagcgcg aggacacatg tcacaataat gacaaaagaa
      181 ttacttaata actgggacat tatattttgc cccatattat cactatcgcc atttacgcgc
      241 gtaacgataa atcctgcgct aatagcaatt aataaagctg gaatttgaga aacaagacca
      301 tcaccgacag taagaattgt gtagacagtc aacgctgttg agaaatccat tccattttgg
      361 cctactccta ctgatagccc accgataata ttaacaaaga taataataat gtttgcaata
      421 gcatcacctt tgataaattt catcgcacca tcaaaggaac catacagctg actctctcgt
      481 tctaatacgc tacgacgttc ttttgctaaa tctgcatcaa tgatcccagc gcgtagatcg
      541 gcatcaatgc tcatttgttt acctggcata ccgtcaagcg aaaagcgggc agcaacttca
      601 gccacacgtt ccgaaccttt agtaatcacg ataaactgaa caatagtaac aatggagaag
      661 ataacaaaac caactaccag gctatctcca ataacgaact caccaaaact cgttataatt
      721 tctccagcat ccgcctcaag aagtataagt cgactggtac tgatcgccag cgccaaacga
      781 aataacgttg tgataaggag gattgatgga aatgaagaaa agcttaagat cctatcaaca
      841 tagaatgatc ctaaaaatac aagtattgcc aatgttaagt taagcccaat caaaaagtcg
      901 attaaatagg tgggcaatgg aattatgagc atggcgataa tcatcaccat taatccaaga
      961 attatt
//
LOCUS       DAZUCE010000510          949 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZUCE010000510 DAZUCE010000000
VERSION     DAZUCE010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.513"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>949
                     /locus_tag="ACHY03_005319"
     CDS             <1..>949
                     /locus_tag="ACHY03_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFS3908396.1"
                     /translation="TDKSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTL
                     TENTRKLTLTEKPWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVA
                     AKKVLLTLWDYMSTPDQIKRMQDIISNPKRLDGIPGQMNAVHIRFDSNSPVMADVQEE
                     GKPQLWNHKQNDALGLYLDLLIQAINTSTINAEDWQKGDRLKSVALLIAYLDKANFYV
                     MEDSGAWEEDARLNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKVNELDETLST
                     TRLNHLIDKGYERITLQLDLGGESPGYLEKDKHYREADAA"
BASE COUNT          310 a          191 c          210 g          238 t
ORIGIN      
        1 caacagataa aagttattta tcgttaaatc cagataagta tcctaattat cgtcttctga
       61 ttcactcagc caaattacaa aatgaaataa agagccacta taccaaagac gaaattcaag
      121 gattgctaac attaacagaa aacacacgaa agttaacgtt gacggagaaa ccctggggaa
      181 cgtttattct tgcttctact ttcgaagatg acaaaacagc ggcagaaacc cactatgatg
      241 ctgtctggct gagggatagt ttatggggct atatggcatt agtatcagac cagggaaata
      301 gtgtagccgc aaaaaaggtt ctgctcaccc tgtgggatta tatgtctacg cccgaccaga
      361 ttaagcggat gcaggacatc attagcaacc caaaacgact ggatggtatt cccgggcaaa
      421 tgaatgctgt gcatatccga tttgacagca actcccccgt gatggcagat gtgcaggaag
      481 aaggcaagcc acagctgtgg aaccataaac aaaatgatgc gttaggtctt tatcttgatc
      541 tccttataca agcaatcaat actagtacta ttaacgctga agattggcaa aaaggtgacc
      601 gattaaaatc ggttgcgctg ctgattgctt atctggataa agccaatttc tatgttatgg
      661 aagattccgg agcgtgggag gaagatgccc gattaaacac atcttccgtt gcactggtga
      721 cctctggact ggaacgtttg tcaaacctac tctcgaagaa agactctgtt tttgtctctg
      781 atttactacg cgaagctaaa gtcaatgaac tggatgaaac tctttcaaca acgaggttga
      841 accacctcat agataaaggt tatgaaagaa taacgttaca gcttgattta gggggagaat
      901 ccccaggtta tctggaaaaa gataaacatt acagagaagc cgatgcggc
//
LOCUS       DAZUCE010000511          940 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZUCE010000511 DAZUCE010000000
VERSION     DAZUCE010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.514"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            90..761
                     /gene="queE"
                     /locus_tag="ACHY03_005320"
     CDS             90..761
                     /gene="queE"
                     /locus_tag="ACHY03_005320"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFS3908397.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          249 a          206 c          235 g          250 t
ORIGIN      
        1 aaacctgcct gtaacagaat gttaataaga catgattgtt gtggcaggat ctgcaataat
       61 taacaattat ccacttcaca gagaatgcta tgcagtaccc gattaacgag atgttccaga
      121 ccctgcaagg tgagggttac tttaccggcg tccccgccat ttttattcgt ttacagggat
      181 gcccggttgg ctgtgcctgg tgcgacacca aacacacctg ggaaaagctt gaggatcggg
      241 aagtctccct tttcagcatt ctggcgaaga ccaaagagag tgataagtgg ggggcggcga
      301 gcagtgaaga cttgctggct gtcattggtc gccagggata taccgcgcgg catgtggtga
      361 ttacgggtgg tgagccttgc attcatgatt tgctgccact gactgacctg ctcgaaaaga
      421 acggttttag ctgccagatc gaaaccagtg gtactcatga ggtacgctgc acaccgaata
      481 cctgggttac cgtatcgcca aagctgaaca tgcgcggcgg ctatgaagtg ttgtcacagg
      541 cactggagcg agccaacgaa atcaagcatc cagtgggacg cgtacgtgat attgaagcac
      601 tggatgaact tttggcgacg ctgaccgatg ataaaccgcg agtcattgca ctgcagccga
      661 ttagccaaaa ggatgatgcc acacgtttgt gcattgaaac ctgcattgcg cgtaattggc
      721 gtttgtcgat gcaaacccat aaatatttaa atattgcctg attaaacatt tataagcgtt
      781 ataaatgggt ggaacctgta aagacttcta ctcatttata ttctttgtcg tctctgaaaa
      841 acctccattt tgcccatcct gaactaatca ttatcattct ctacaaattc tgtggcgtta
      901 atttttcgct ggagtgaaaa ttattgcggt aaagtttggt
//
LOCUS       DAZUCE010000512          940 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZUCE010000512 DAZUCE010000000
VERSION     DAZUCE010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.515"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            57..209
                     /locus_tag="ACHY03_005321"
     CDS             57..209
                     /locus_tag="ACHY03_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFS3908398.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTRSKTFKTEAAARRW
                     LARNSD"
     gene            complement(323..838)
                     /locus_tag="ACHY03_005322"
     CDS             complement(323..838)
                     /locus_tag="ACHY03_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908399.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQHECVSLVELVSTYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
BASE COUNT          259 a          215 c          205 g          261 t
ORIGIN      
        1 acagcgcagc aggtttttag ttccgcgccc cggcgttaag gggaaatgag gtcaacatgg
       61 atacgctcaa tcttggcaac aacgaatctc tggtatgcgg cgtgtttccc aaccaggacg
      121 gcacgtttac cgcgatgacg tataccagaa gcaaaacgtt taaaactgaa gctgccgcgc
      181 gtcgctggtt agcaagaaac tctgactaat gaagtctggt agttaaggaa tcctccacgg
      241 ggagaactgg tgcacacgcg ccggacacaa gcaagcatcc ggcatgctct ttaacaatct
      301 ggatatccat aacagtaaca atctacagat tgccgttcag ttttctggcc aactcctcaa
      361 tagatggagg cgatacataa tccggatttt tattcataag aaactgattt tcacagtaga
      421 ggcacctgct tttatgaaaa aactcatatt ctctaaccgg gaatggttga agtatcgata
      481 ctatcttttg tccaaaacat tttggacaaa gatgcatgat tatgctgccg ccgttcacgg
      541 ttacctcctt cgagtacaca aaagttcccg actcaagttg gttaaggata taaccttccg
      601 tctgagcctc aaagttttca aattctgcaa ttttagcttt gagagaagca tttatttctt
      661 gataagtgct caccagctca acgagagaca cgcattcgtg ctgaatagat gcaagctttg
      721 agttcagttc accaatagcc gcatttactt cagcttgagt ttttgcctcg ttcattagtt
      781 ttgcaatctg agcggtttca cgaatagccg tcattgctgc cgttaattca gcgatcacat
      841 tcaatactct tattgttgtt ggggatatcc agattaacaa gatccttgtt gttggggaat
      901 aacaggtcca cctcgcctga cgtggttaaa agcaggcaca
//
LOCUS       DAZUCE010000513          933 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZUCE010000513 DAZUCE010000000
VERSION     DAZUCE010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.516"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            30..>933
                     /locus_tag="ACHY03_005323"
     CDS             30..>933
                     /locus_tag="ACHY03_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFS3908400.1"
                     /translation="MKINRHGITLSLISFLIAGAPAFAQDDSSWASPGTASASETSST
                     AVGAWARAGKAGTALGSASYAHAPFSTSVGMSAYVNPKASNGVAVGSWAKADALNSVA
                     LGTGAQATHRYSVALGQQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTR
                     DTDAATVGQLNRKADEVYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNT
                     RGLLSQRDVLETHEERLNSQQQQINTGSTVAVDSHGYVTRGEGTGERITVQEGLVRTQ
                     EMATENRAAVSRNRQVGERNSRAIA"
BASE COUNT          228 a          216 c          291 g          198 t
ORIGIN      
        1 gtatattgtg tatatcaatg aggttgttta tgaaaataaa tcgacacggt attactttat
       61 cgcttatttc ctttctgatt gccggtgctc cggcgtttgc gcaggatgac agttcctggg
      121 cgtctccggg cacagcctct gcatcagaga ccagctccac ggctgtaggt gcatgggcga
      181 gggctggaaa ggcagggaca gctcttggct ctgcatcgta tgcgcatgcg ccgttttcca
      241 catccgtggg gatgtcagct tacgtaaatc cgaaggcgtc taatggtgtg gcagtaggta
      301 gctgggcaaa ggctgatgct ttaaattcgg tggcactggg tacgggtgcg caggcaacgc
      361 acagatattc agtggctctt ggccagcagt ccggcacaac ccgtgaaaat gaagtctaca
      421 tcggatatga ctcaggtgtc acaaacccgg ccagtccgcg tgtaccggat aaaacccggg
      481 ttctcggtgg tgtcagtgat ggtacacggg atacggatgc agccactgtc gggcaactga
      541 accggaaggc ggatgaggtg tacagtgacg tcagtggacg gattgcagcg gaagcactta
      601 aggcgcgtga ccataccgat acggttgctg cagagaacag ggagaatatt atccggaaca
      661 cggtggccat aaaccgtaac accagggggt tgctttccca gagagacgtg ctggagacgc
      721 atgaagagcg cctgaacagc cagcaacagc agataaatac cggaagtacc gttgctgtgg
      781 acagtcatgg ttatgtgacg cgcggtgagg gaaccggtga acgaattact gtacaggaag
      841 ggctggtacg tacacaggaa atggccacag aaaaccgtgc tgcggtaagc cgtaaccgtc
      901 aggtgggtga acgcaacagt cgcgccattg ctt
//
LOCUS       DAZUCE010000514          932 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZUCE010000514 DAZUCE010000000
VERSION     DAZUCE010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.517"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>932
                     /locus_tag="ACHY03_005324"
     CDS             <1..>932
                     /locus_tag="ACHY03_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS3908401.1"
                     /translation="VLQPGGLRKTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDM
                     TGAWFWRVLWRFRPYYGQVILASFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSI
                     GVIAAILFECAAKLIRSYLTDIAGKKADLIISSALLRRVMAVKMSDRPASSGSYASNL
                     REYESVREFMTSATLLAFVDLPFILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQR
                     PLSRHINASMRESSQRQGLLVEALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVK
                     NTSNVIICFSTAVQQLNTVLLVILGTYLIHSDDP"
BASE COUNT          197 a          216 c          260 g          259 t
ORIGIN      
        1 tgtactccag cccgggggac tccgtaagac actttctcac gatgaattaa tggctgttta
       61 ttcaggttac tgctggtttg ttcagccaca ggcaacacag gatcgccgtt cggaactgcc
      121 ggagtatgac atgaccgggg catggttctg gcgggtactg tggcggttca ggccatatta
      181 cggacaggtc attcttgcga gttttatcat taactttctt gcgctggtca gttccctgta
      241 tgtcatgaac gtttatgacc gggtcatacc caatcgctcc tgggaaacgt tatggatgct
      301 gagtatcggg gtgattgcag ccattctgtt tgagtgtgct gcaaagttaa tccggagcta
      361 tctgacggat attgcgggta agaaggcaga cctgattatc agctcagcac tgttaaggcg
      421 ggtgatggca gtgaaaatgt cggacaggcc ggcgtcttcc ggttcatacg ccagcaattt
      481 gcgggaatat gagtctgtca gggagtttat gaccagcgcc acgctgctgg cttttgtgga
      541 tttgccgttt atcctgcttt tcatcggtgt catcgggctt gtggggggct ggcttgctgt
      601 tgttcctctt acactggttc cgctggtgat tctggcagga atattactgc agcgaccgct
      661 ttcgcgtcat attaatgcct ccatgcggga atcatcgcag cgccagggat tacttgtgga
      721 ggcactggac ggaatagaaa ccctgaagct gaataatgca gttaactggg tccagcgccg
      781 ctgggatgga tatacagcca gtgtcgcccg ctcgtccata cgggtaaaaa ataccagtaa
      841 tgtcataatc tgcttttcca cagcagttca gcaactgaat actgttctgc tggtcattct
      901 ggggacgtac cttattcaca gtgatgaccc ac
//
LOCUS       DAZUCE010000515          923 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZUCE010000515 DAZUCE010000000
VERSION     DAZUCE010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.518"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            50..>923
                     /locus_tag="ACHY03_005325"
     CDS             50..>923
                     /locus_tag="ACHY03_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFS3908402.1"
                     /translation="MVRSQNALRMIAGDYGVIWRNDGANTYLLMTDKGDQYGGWNGLR
                     PFAVNNATGEVTINTPLNSPKGIKGNSDTATKLQTAIKISGVSFDGSTDITLTAAHVA
                     AFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRN
                     GGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQTFD
                     KSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGT
                     KTTSSFDYGTKSTNN"
BASE COUNT          249 a          211 c          235 g          228 t
ORIGIN      
        1 gagtaagtca gacattagcg ggaataagat ttacgctaat gatgaagtta ttgtccgtag
       61 tcagaacgcg ttaaggatga ttgcaggtga ttacggtgtc atatggcgaa atgatggagc
      121 aaatacttac ttgctcatga ctgataaagg cgaccagtac ggtggctgga acggtcttcg
      181 accatttgca gtaaataacg caacaggtga agtgacgatc aatacaccac ttaattctcc
      241 taaggggatt aagggaaatt ctgatacagc tacgaaactt cagacagcaa taaaaatatc
      301 tggtgtttcg tttgatggtt ctactgatat cactttaacc gctgcgcatg ttgctgcttt
      361 tgccagaaga gcaacggata cgtatgccga tgcggatggg ggcgttccct ggaatgccga
      421 atcaggcgct tacaatgtca cccgctctgg cgacagctat attctggtta acttctatac
      481 cggagtcgga agttgccgga ccttgcagat gaaggcgcat tacagaaatg gtggtctgtt
      541 ctaccgttct tcaagagacg gttatggttt tgaggaagat tgggcagaag tttatacctc
      601 gaaaaatctt ccaccagaaa gctacccagt cggcgcacca atcccgtggc catcagatac
      661 cgttccgtct ggttatgccc tgatgcaggg gcagactttt gacaaatctg cctacccgaa
      721 acttgcagcc gcttatccgt caggcgtgat ccctgatatg cgtggctgga cgattaaggg
      781 caagcccgcc agtggtcgtg ccgtattgtc tcaggaacag gacggcatta aatcgcacac
      841 ccacagcgcc agcgcatcca gtacggattt gggtacgaaa accacatcgt cgtttgatta
      901 cggcactaaa tccacgaata aca
//
LOCUS       DAZUCE010000516          921 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZUCE010000516 DAZUCE010000000
VERSION     DAZUCE010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.519"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..733)
                     /locus_tag="ACHY03_005326"
     CDS             complement(<1..733)
                     /locus_tag="ACHY03_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3908403.1"
                     /translation="MAENKIYAVLTDRGAQLEAAALASGVPVVLNKFVIGDANGNDDV
                     TPDPARTALIHETYRGDIKSSENSGNQVIFTLYVPPETGGYTIREVGILTDKGELYSV
                     ARSPDILKPTDSNGALISITYKYTLAVSSTSTVNVVIDNSSGMSQADADKRYLQISKN
                     LSEIKDKGAAAQKTARENIGVNLDDYYTKKDVDNLIDDSDDLAPVVPAFAAVGSVVYA
                     GFGFSNETVMHYKPGDTIAGSLIFPA"
     gene            complement(726..>921)
                     /locus_tag="ACHY03_005327"
     CDS             complement(726..>921)
                     /locus_tag="ACHY03_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFS3908404.1"
                     /translation="CRSLLKEINFETGVDGEFFIAGTPYAKIGVNIPADGDGGVKLNG
                     GLYISGSPIVSLNVEIGPNG"
BASE COUNT          238 a          233 c          178 g          272 t
ORIGIN      
        1 acgcaggaaa aatcaatgaa cctgcaatag tatccccggg tttatagtgc ataacagtct
       61 cgttactaaa tccaaatcct gcataaacaa ctgagccgac agctgcaaac gcaggcacca
      121 ccggtgcgag gtcatcgcta tcatcaatta aattgtctac gtcctttttg gtgtaataat
      181 catcaagatt aacgccgata ttttcccgtg ccgttttttg cgccgccgcg cctttgtctt
      241 taatttcaga taaatttttg cttatctgca aatatcgctt atcagcatcg gcctgactca
      301 ttccactact gttatcaata actacgttaa cagtagacgt gctggacacc gcgagcgtgt
      361 atttatacgt aattgaaatc agcgcgccgt tgctgtccgt aggttttaaa atatccggtg
      421 aacgtgcaac tgagtacagt tcgcctttat cggttaatat tcccacctca cggatagtat
      481 agccgccggt ttccggtggt acgtatagtg taaagataac ctggttacca ctattttctg
      541 acgatttaat atccccgcga tacgtctcgt gaattaatgc agtacgcgcc ggatccggtg
      601 ttacgtcgtc gtttccgttc gcgtcaccga taacaaattt gttcagcacc accggcacgc
      661 ctgacgccag cgcagcggct tcgagttgtg cgccgcggtc tgtcagtact gcgtaaattt
      721 tattttcagc catttggccc tatctccaca tttaaactaa caattggtga gcctgatata
      781 tataatccgc catttaattt cacgccgcca tcaccatctg ccggtatatt tacgcctatt
      841 ttcgcgtaag gtgtaccagc tataaaaaac tcaccatcaa caccagtttc gaaatttatt
      901 tcttttaata atgaacgaca a
//
LOCUS       DAZUCE010000517          920 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZUCE010000517 DAZUCE010000000
VERSION     DAZUCE010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.520"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..477)
                     /locus_tag="ACHY03_005328"
     CDS             complement(<1..477)
                     /locus_tag="ACHY03_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFS3908405.1"
                     /translation="MNTAAEAIRALSLQVPGFRGQMNEGWYQIRIAGEDTAPEAVYAC
                     LHEQLGEGAVIHIVPRLAGAGGNGVFQVVLGAAAIVGSFFTAGATMALWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMP"
     gene            complement(474..>920)
                     /locus_tag="ACHY03_005329"
     CDS             complement(474..>920)
                     /locus_tag="ACHY03_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFS3908406.1"
                     /translation="IHRFRCVPHLTGRRFEQGVTDCYTLFRDAYHLAGITLPDFVRED
                     DWWRNGQNLYLDNLADNGFYRVSPSCAQAGDILLCCFGASVPNHAAIYCGNGELLHHI
                     PEQLSKRERYSEKWQRRTHSVWRHRHWSASAFTGIYNDLVAASACM"
BASE COUNT          198 a          293 c          244 g          185 t
ORIGIN      
        1 cggcatcggg ttaccctggg caatcatgtt gtccagtgac gaaaaatacg tgttctgttt
       61 accgttatcc gtacttttgt actccggcgt ctttgccttc ggggccagca tctgggccac
      121 accacccagt atcatgctgg cacccagtga gaacagcatc gtggtggcag tcagccctcc
      181 ggcactcagg gctgcgcccc acaacgccat cgttgcgccg gcggtgaaga aagagcccac
      241 gatggctgct gcccccagca ccacctgaaa aacaccattt cccccggctc cggccagtcg
      301 cggcacaatg tggatgaccg ctccctcacc cagttgttcg tgaagacagg catacaccgc
      361 ctccggcgcg gtatcctcac cggcaatacg tatctgatac cagccttcat tcatctgacc
      421 gcggaatccc ggcacctgca gcgacagggc acggatggcc tccgctgccg tgttcacata
      481 caggctgatg cggcgaccaa atcgttgtaa atccccgtga aggcagatgc ggaccagtgg
      541 cggtgacgcc agacagaatg cgttcgtcgt tgccattttt cagaatacct ctcccgttta
      601 ctcagttgtt caggtatatg gtgaagcagt tcaccgttgc cacagtaaat ggcggcatga
      661 ttcggcaccg atgcgccaaa gcagcacagc aggatatcgc ctgcctgtgc acaggacgga
      721 gacacccggt aaaatccatt gtccgccagg ttgtccaggt acaggttctg gccgttgcgc
      781 caccagtcat cctcacgcac aaaatccggc agcgttatcc ccgccagatg gtatgcatcc
      841 cggaacaggg tgtaacagtc cgtcacaccc tgttcaaagc gccgtccggt caggtgcggc
      901 acacagcgga accggtgaat
//
LOCUS       DAZUCE010000518          902 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZUCE010000518 DAZUCE010000000
VERSION     DAZUCE010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.521"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(82..591)
                     /locus_tag="ACHY03_005330"
     CDS             complement(82..591)
                     /locus_tag="ACHY03_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908407.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGINGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWPVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
BASE COUNT          278 a          225 c          179 g          220 t
ORIGIN      
        1 agtattccgt ataaagattc agctggcaaa actttatcag tctgtaaaaa ctaacggaag
       61 agtcgatatt tctcccgtcc atcaccggat gattgttgca ataccccgtg gcatcagaga
      121 ctgaacagcc gtttttaacg ccacgtattg ccctgatgaa ccaggccggg caacccgaaa
      181 acatctttcc acccggcatt gtccgccagc acttatcacc ggccaggtct gttgcagtaa
      241 atccaccagt tgcgcacatt cttcatcagc catgacgccg actcgcggat ggcaaatact
      301 cgtctggctg agcagcaaca gacatcgtat ctgtgctcct tcatgttgtt cccgcaacca
      361 gcgtaatacc accggcgctg atgggctgac cgtgtgatta tgctccgtaa tacgttcgtg
      421 tacccgtcgg tgaaaggaac tacaaaatgt ctgaatctgt tgaccattga ttccttctgc
      481 cgaatagagc gtgaagtgaa atgccaacag atttggccag tggtccaccg cctgctgaac
      541 aaaacgccgg atttcccccg gctctgaaat taaggcttcg gttatttgca cgaaatgttc
      601 cttttcaacc cctgttgaat tttgtcaagc cggtgcagac acagtaaagc gcgcatcaac
      661 acctgaaagg tacgatacaa acacaagtta caaacaataa gaacggaagg acaattaatc
      721 agtgaggaat aataactaaa ttaaccaaaa gaatgttact gacaatacta ccaatactta
      781 aatagcaata tattaattaa tgtaagaaca gatgcccggt aagacatata cacaatacat
      841 ccacaaacac actcttttca ttaaatcaat cacctgatta aaccatgaaa gtgtgatgta
      901 ag
//
LOCUS       DAZUCE010000519          902 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZUCE010000519 DAZUCE010000000
VERSION     DAZUCE010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.522"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            45..578
                     /locus_tag="ACHY03_005331"
     CDS             45..578
                     /locus_tag="ACHY03_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3908408.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            628..>902
                     /locus_tag="ACHY03_005332"
     CDS             628..>902
                     /locus_tag="ACHY03_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFS3908409.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRK"
BASE COUNT          292 a          172 c          180 g          258 t
ORIGIN      
        1 ttgaaaacga gtgaccttcc ataccagtta attaaggatg atttatgaaa aagtatatta
       61 ttcccttcgt tgcgacaacg ttgatgttta gcgcaacaac cttcgccaag gatgtcgatc
      121 aaggaacact aaccattaga ggagaagttg ttgatacaac gtgttacttt gttaatggtg
      181 attcgacaac agatatcgtt gtcgaagatg ttgtaaccag tgcaatgaat caacttatca
      241 ataatgaaat ctatacttta aaaaccggtt caacctctca tgacttagaa attcagtgtg
      301 acggaaatac agccccaagt atcgaagtac tagcatcaga gtttgatgct aataacttta
      361 cgctgaataa aggaactgct gagaacattg gctttgccat tttcctcgat gatgggactt
      421 tgaccagaat gagaccaggg ttaaaaacac cgctgaaaga taatggctct ggtcaatatt
      481 ttcttaattt gaatgcacaa tatgcccgca caagtggcaa tccagttaca aaaggctccg
      541 tagagtcaac cgtaacgatt aaaatcagtg cagattaacg ttttttgccg gtagctctgc
      601 taccggtatt atttacggag tgattacgtg agaataataa cagcattact gatgtcattt
      661 tttattttac ctgttcatgc cggtgtggtc atctatggta cgcgcattat ttatcctgaa
      721 aaaaacagag aagttctcgt acagttaatg aatcaaagta aaaatgcctc tttaattcag
      781 gcatggatcg atgatgggaa caccactatc gcgccagaaa aaattcaggt cccatttatt
      841 ctcacaccgc cagtaagtcg tgtagcaggt ggttctggcc aacaacttaa aataagaaaa
      901 at
//
LOCUS       DAZUCE010000520          884 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZUCE010000520 DAZUCE010000000
VERSION     DAZUCE010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.523"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..385
                     /locus_tag="ACHY03_005333"
     CDS             <1..385
                     /locus_tag="ACHY03_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908410.1"
                     /translation="TGRVAGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQEQVTWL
                     AGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSIIRQRPV
                     MEYDASHLGKMVNRLEEALLPELPA"
     gene            461..>884
                     /locus_tag="ACHY03_005334"
     CDS             461..>884
                     /locus_tag="ACHY03_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFS3908411.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPL"
BASE COUNT          236 a          218 c          223 g          207 t
ORIGIN      
        1 gacagggcgt gtggctggcg gtgttctggg gggaatgatt gcctccgctg tatcaggaaa
       61 aattgccgga gcgctggttg aagaagatcg cgtcaaaatt ctggcaatga ttcaggagca
      121 ggtgacatgg cttgccggca gtttcctgct gaccggacat gagattgaaa atctgaacgc
      181 gaatctggcc cgtgttatcg atcagaatgc tctggagatc attttcgccg ccggtataca
      241 acaacgggcc gcgaccaata tgttaatcaa accactggtg gtcagtatca tcaggcaacg
      301 ccccgtcatg gaatatgatg catcccatct cggcaagatg gttaaccgac tggaagaagc
      361 attactcccg gagttaccgg cataacggct attctgcgcc ttttacgttt tatcacctga
      421 tttgtcagtg tcatcgtcat gactcagaga ggtaaatacc atgattcacc tgttcaaaac
      481 ctgcatgatt accgccttca ttctggggtt aacgtggtct gccccactcc gggcacagga
      541 tcaacgttac atcagtatac gcaatacaga tacgatatgg ctcccgggaa atatttgtgc
      601 ttaccagttc cggctggata atggcggaaa cgatgaagga tttggccccc tcaccatcac
      661 tctgcaactc aaagacaaat atggtcagac gctggtgacc agaaaaatgg aaacggaagc
      721 ctttggtgac agtaatgcca cgcgaaccac agacgcattt ctggaaacgg agtgcgtgga
      781 aaatgtcgcc acaaccgaaa tcattaaagc aactgaagaa agtaacggcc atcgtgtcag
      841 tctgccgtta tcggttttcg atccccagga ctaccatcca ctgc
//
LOCUS       DAZUCE010000521          872 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZUCE010000521 DAZUCE010000000
VERSION     DAZUCE010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.524"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            242..844
                     /gene="fimB"
                     /locus_tag="ACHY03_005335"
     CDS             242..844
                     /gene="fimB"
                     /locus_tag="ACHY03_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFS3908412.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          253 a          164 c          196 g          259 t
ORIGIN      
        1 tagattcgta tccgattgat aaatatataa aggtacatag catgcaagag catagcgttt
       61 gtatggcaac gttattataa gtaactgttg ctactccatt taagttcact cagaagaact
      121 ggtccactta cgttagttat taagcaaacg ttcgctttta taaacataat caggataaaa
      181 atgttggatt attgctaacc cagcacagct agtgcgcgtc tgtaattata agggaaaaac
      241 gatgaagaat aaggctgata acaaaaaaag gaacttcctg acccatagtg aaatcgaatc
      301 actccttaaa gcagcaaata ccgggcctca tgcagcacgt aattattgtc tgactttgct
      361 ttgttttatt catggtttcc gggcgagtga aatttgtcga ttgaggattt cggatattga
      421 tcttaaggca aagtgtatat atatccatcg attaaaaaaa ggcttttcaa caacgccccc
      481 gctattgaac aaagaagttc aggctttaaa aaactggttg agtatccgta cttcttatcc
      541 gcatgctgag agcgagtggg tatttttatc gcgtaagggg aatccgcttt ctcggcaaca
      601 attttaccat attatctcga cttccggtgg taatgccggg ttgtcactgg agattcatcc
      661 gcacatgtta cgccattcgt gtggttttgc tttggcgaat atgggaatag atacgcgact
      721 tatccaggat tatcttgggc atcgcaatat tcgtcatact gtctggtata ccgccagcaa
      781 tgcagggcgt ttttacggca tctgggatag agccagagga cgacagcgtc acgctgtttt
      841 atagagatag ttgagatacc agggatggtg tt
//
LOCUS       DAZUCE010000522          862 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZUCE010000522 DAZUCE010000000
VERSION     DAZUCE010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.525"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            166..402
                     /gene="ycgZ"
                     /locus_tag="ACHY03_005336"
     CDS             166..402
                     /gene="ycgZ"
                     /locus_tag="ACHY03_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFS3908413.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            445..717
                     /gene="ymgA"
                     /locus_tag="ACHY03_005337"
     CDS             445..717
                     /gene="ymgA"
                     /locus_tag="ACHY03_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFS3908414.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRSDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            746..>862
                     /gene="ariR"
                     /locus_tag="ACHY03_005338"
                     /gene_synonym="ymgB"
     CDS             746..>862
                     /gene="ariR"
                     /locus_tag="ACHY03_005338"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFS3908415.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVT"
BASE COUNT          292 a          139 c          177 g          254 t
ORIGIN      
        1 aggttgtcat ttaaatgatg caataatttt taccataact tgttgtttta tatgcattag
       61 cactaaatgc aaaaaattaa tttatcattc tgtacacata tttcgtacaa gtttgctatt
      121 gttacctcac ttaacattga ttaacatttt taacagaggc gtagcatgca tcaaaattca
      181 gtgactttag attctgcggg agcaatcact cgttactttg caaaagctaa cttgcctact
      241 cagcaggaaa ctctcgggga aattgtgact gagattttga aagatggacg taatctgagt
      301 cgaaagtcgc tttgtgccaa acttctttgc cgactggaac aagcgacagg ggaagaggaa
      361 cagaaacatt ataatgcact aattgggttg ctttttgaat aatgcaacag cctgattatt
      421 taacaggtta gctatgaagt cgttatgaag acatctgata atgaaagtat aaaatatgaa
      481 attactggcc aggcggtgct ccagatactg cgcatgaaga taaatttttc attgcagact
      541 cttattaagc aattactcgt aatgaaatcg gctgaggaag atgctttccg aagcgattta
      601 attgacagca taattcgtga ttttagcaat agtgattcag gagggccaaa ccgaagaaca
      661 gcgacagctg acaataaaag tatgttcaat ggtaagaaaa taaacagaat acattaaaat
      721 ttcataagta agatgagagg ttaccatgct tgaagatact acaattcata atgcaataac
      781 tgataaagcg ttagcaagtt actttcgcag ttcgggtaat ttgttagaag aagagtcagc
      841 agtgttaggg caggctgtca cc
//
LOCUS       DAZUCE010000523          856 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZUCE010000523 DAZUCE010000000
VERSION     DAZUCE010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.526"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..560
                     /gene="ydeO"
                     /locus_tag="ACHY03_005339"
     CDS             <1..560
                     /gene="ydeO"
                     /locus_tag="ACHY03_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFS3908416.1"
                     /translation="TVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLF
                     SCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKK
                     LKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPK
                     RVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT          267 a          159 c          163 g          267 t
ORIGIN      
        1 agacagtgac gatgcctgtt cctgcgaagt ttttacgtgc ttctaatgta ccgactggat
       61 tgcttaatga aatgattgct tatctcaact cggaagaacg caatcatcat aatttttcag
      121 aacttttgct tttttcttgc ctgtctattt ttgccacatg caaaggtttc attacactat
      181 taactaacgg tgtgctatcc gtttcgggga aagtgagaaa tattgtcaac atgaagctgg
      241 cgcacccatg gaagctgaaa gatatttgtg actgcctgta catcagtgaa agcctgttga
      301 agaaaaaact aaagcaagag caaacgacat tctcacagat tcttttagat gccagaatgc
      361 agcacgcaaa aaatttgata cgcgtagaag gttcagtcaa taaaattgcc gaacaatgtg
      421 gttatgccag tacatcttat tttatttatg cgttccgcaa acatttcggc aacagtccga
      481 agagagtttc taaggagtac cgttgtcaaa ggcacacggg tatgaatacg ggcaacacga
      541 tgaatgcttt agctatttga ttatttgcta acgagtagtc aaccacacac gctacgtaaa
      601 aattaaaaat ggggcagtca ttccctgccc cgcgttgttt ttaggcgata tatttattga
      661 aataaataag tgacatccat cacatattta tgcatttgca taacctgttg catgattatt
      721 tatgatctca attcggcgtt ttgtcagcaa aaatacaata atttattaaa tatcaataga
      781 ttagttgttt atcatcgaga aattacttag tataacagaa agtaatgtca acgctttatg
      841 gactgctatt tccctt
//
LOCUS       DAZUCE010000524          856 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZUCE010000524 DAZUCE010000000
VERSION     DAZUCE010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.527"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>856)
                     /locus_tag="ACHY03_005340"
     CDS             complement(<1..>856)
                     /locus_tag="ACHY03_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS3908417.1"
                     /translation="AVILSGRALAPLGQIASLATRFQQARLAMQGVNDIVRRPVERES
                     QTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGSIGCGKSTLL
                     KMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRENLQLSRLPG
                     SLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEGGQGLSGGQRQMVALTRMVLGSPRV
                     VLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVVMNEGRVMMD
                     GPRDEILKK"
BASE COUNT          217 a          268 c          193 g          178 t
ORIGIN      
        1 gttttttcag tatttcatca cggggaccgt ccatcattac ccgcccttca ttcatcacga
       61 caatacggtc aacaatattc agaacctgca gacgatgtgt caccaccacc attgtgcgtc
      121 cacggctcca gtggctcaga cctttcagaa caacagactc agtctgcagg tccagacctg
      181 tcgtgggctc atcaagcagt accacccggg gagaacccag taccattctc gtcagggcaa
      241 ccatctgcct ctgtcctcct gacagtccct gaccgccttc ccctgtctgc atatcaagtc
      301 cgcggggatg atgccggata aaacggtcaa acccgaaacg tccggctgca ttaatcatat
      361 ccacatccga caggcttccc ggcagacgcg aaagctgcag attttcacgc agagttccca
      421 gaaaaagccg gggattctgt tccagaagaa acactttatc ccggacaaaa caggggtcca
      481 gttgctgcat cgcaaccccg tcaagagtaa tttgtcccct gttctgacgg tacagcccca
      541 ccatcatttt cagcagcgtc gatttaccac aacctattga gccaataata cccacccgct
      601 ctccgggccg gatacagagc gacagaccgc acaatgcgtc gggtaatccg ggcttataag
      661 aaaaggagac atcactgaaa cgaaactctc cctggatatc cttcactgtc agataatgtg
      721 tctgactttc ccgctccacc ggacggcgga caatgtcatt caccccctgc atggccaggc
      781 gggcctgctg aaaacgggtg gccagtgatg ctatctgacc aagcggtgcc agcgcacgac
      841 cggaaagaat aactgc
//
LOCUS       DAZUCE010000525          856 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZUCE010000525 DAZUCE010000000
VERSION     DAZUCE010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.528"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>856
                     /locus_tag="ACHY03_005341"
     CDS             <1..>856
                     /locus_tag="ACHY03_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFS3908418.1"
                     /translation="PASLAAQNTNVVEVDETAKGHQVYGDNWKSIEIGTTKEVKKKDS
                     TDLNFDHNSPVAKGHNSIAIGTSAEAGGEIKDEKGFKTGESIAIGAKAKATKEQSIAI
                     GGDTKATGWGAIVIGGDDLTPLHGDRFGDKKIPNDYSSSEASGDGSIVVGSRSTAAGD
                     LSVSLGSISSANGTAANALGATAEANGDMATAVGFNALANASNALAVGSQASAGGEDS
                     LAIGSQSTTGKKSTVALGQGATASAAEGNVAIGADSVDKAATPVSGATIKLKNGGTIE
                     YRGFAGDKA"
BASE COUNT          245 a          174 c          253 g          184 t
ORIGIN      
        1 tccctgcatc actggcagca caaaacacta atgtagttga agttgatgaa acagctaaag
       61 gtcaccaagt ttatggtgac aactggaaaa gtattgaaat aggtacaacg aaggaagtaa
      121 agaaaaagga ttcaaccgac cttaattttg accataactc cccggtagcg aaagggcata
      181 actccattgc gattggtacc agtgcagaag caggaggtga aattaaagac gaaaaaggtt
      241 ttaagacagg agaatccata gctattggtg ccaaggcgaa agcaactaaa gaacagtcaa
      301 ttgcaatagg tggcgatacc aaagctaccg gatggggggc tattgttatt ggtggcgatg
      361 atttaacccc tttgcatggt gacagattcg gtgataaaaa aattccgaat gattactcgt
      421 catctgaagc atcaggagac ggaagtattg ttgtgggctc aaggagcaca gctgcaggag
      481 acctgtctgt gtccctgggc tcaataagct ctgccaacgg gacggctgct aatgcactgg
      541 gagccacggc ggaagctaac ggggatatgg cgactgcggt tggttttaat gcgctggcta
      601 atgccagtaa tgcgctggct gttggtagtc aggcctctgc cggcggagag gattccctgg
      661 ctatagggag tcagagtacg acgggcaaaa agtccacggt tgctctgggg cagggagcta
      721 cagcctctgc cgcggagggg aacgtggcca tcggtgctga ttcagtggac aaggcagcaa
      781 ctccggtttc cggcgctacc attaagttaa agaatggcgg gactattgaa tacaggggct
      841 ttgccggaga taaggc
//
LOCUS       DAZUCE010000526          846 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZUCE010000526 DAZUCE010000000
VERSION     DAZUCE010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.529"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            33..665
                     /gene="yqeH"
                     /locus_tag="ACHY03_005342"
     CDS             33..665
                     /gene="yqeH"
                     /locus_tag="ACHY03_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3908419.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          276 a          143 c          163 g          264 t
ORIGIN      
        1 tgcaattata tacggatagg gaggttctta acatggggct ttgtagtcgt tataaaagtc
       61 ttacatgcaa tagttgctca atgcattgcc aaataatgcc agaagagtca ccgcgtttgc
      121 agtattgcgc aaactcgtgt ttttgtatgt ggcccgaaga aagttcatat tttaatcgtg
      181 gtgtggtaga aggtatctta acaaaaaacc acaatgcgag attaagcgga tatatttttg
      241 tcgatttttc agtaagtttt ttacgtctat tccttgaaaa agactggatt gactatcttg
      301 caagtactga catggggatt gttttagtca gcgaccgcaa tatgcaatcg ttagctaatt
      361 actggcgaaa acacaactct gcaatatctg ctgtcatata taatgatgat ggtcttgatg
      421 ttgccaatga aaagatcaga caactattta ttggtcgtta tttatcattt accagaggta
      481 acacattaac tcagatggaa ttcaccataa tggggtatat ggtttctggt tataatccat
      541 atcagattgc tgaagttctg gatatggata tccgtagcat ctacgcgtac aagcaacgaa
      601 tcgaaaagag aatgggtggt aaaataaacg aattatttat tcgttcacat tcggtccaac
      661 attgatactc aaaaatctgc cagaatcata ctctgtttat acgggcattt attgaaatgc
      721 ccgtatttta taaataggcc gtcccctcaa gagtaaacat catagtattt aaaaccatca
      781 cacgaaaaat tcagaagcat tacgtaatat aaatttacaa acaatagtgg cataaatgtt
      841 aaccat
//
LOCUS       DAZUCE010000527          838 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZUCE010000527 DAZUCE010000000
VERSION     DAZUCE010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.530"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>838
                     /gene="ehxA"
                     /locus_tag="ACHY03_005343"
     CDS             <1..>838
                     /gene="ehxA"
                     /locus_tag="ACHY03_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFS3908420.1"
                     /translation="VYDYSNLIQFVEKDNKHYQARIISELGDKDDIVYSGAGSSEVFA
                     GEGHDTVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRT
                     EKIQYRDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEG
                     NYGNDRLYGDDRDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAY
                     NILSGGTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGD
                     RLH"
BASE COUNT          272 a           99 c          228 g          239 t
ORIGIN      
        1 gtatatgatt attcaaattt gattcagttt gttgaaaagg ataacaaaca ctatcaggcg
       61 agaataattt ctgagctcgg agataaagac gatatagttt attctggggc aggctcatca
      121 gaagtatttg ctggtgaagg tcatgatacc gtatcttata ataagacgga tgttggtaaa
      181 ctaacaattg atgcaacagg agcatcaaaa cctggtgaat atatagtttc aaaaaatatg
      241 tatggtgacg tgaaggtatt gcaggaagtc gttaaggaac aggaggtgtc agtagggaag
      301 cgaacagaga aaatacaata tcgtgatttt gaattcagaa ccggtggaat tccttatgat
      361 gtgatagata atcttcattc tgttgaagaa ctcattggcg gaaaacatga tgatgaattc
      421 aaaggcggta agtttaatga tatattccat ggcgcagatg ggaacgatta tatcgaaggt
      481 aattatggta atgatcgact atacggcgat gatagggatg attatatatc cggaggacag
      541 ggagacgacc agttatttgg tggtagtgga aacgataaat tgagtggagg ggatggtaat
      601 aattatctga caggaggaag cggtaatgat gagcttcagg cacacggagc ttataatatt
      661 ctgtcaggtg gtactggtga tgataaactt tatggtggtg gtggtattga ccttctggat
      721 ggaggggaag gtaatgacta tctgaatggt ggttttggta atgatattta tgtttatagg
      781 caaaactatg gtcatcatac aattgcagat gaaggaggta aaggagatcg tctgcact
//
LOCUS       DAZUCE010000528          836 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZUCE010000528 DAZUCE010000000
VERSION     DAZUCE010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.531"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..335)
                     /locus_tag="ACHY03_005344"
     CDS             complement(<1..335)
                     /locus_tag="ACHY03_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908421.1"
                     /translation="MSFLSEQDVQNLVINKMIFHVVGKNLEAPTLLKEIKPVQHVDFF
                     LERIKSSLKGNLFTFLENSNTERILRIIRDKADAEANCFTEQSKLLASDFQSHHSSGN
                     TSMGCFFLF"
     gene            665..820
                     /locus_tag="ACHY03_005345"
     CDS             665..820
                     /locus_tag="ACHY03_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFS3908422.1"
                     /translation="MKQQKAMLVALIVICLTVIVTVLVTRKDLCEVRIRTDQTEVAVF
                     TAYELGE"
BASE COUNT          252 a          165 c          193 g          226 t
ORIGIN      
        1 tcaaataaga aaaagcatcc catactcgtg ttaccgctag aatggtgtga ctgaaaatca
       61 ctggccagta gcttcgattg ttctgtaaaa caattagctt cagcatctgc tttatctcta
      121 atgatacgca ggattctttc ggtatttgaa ttctccagaa aagtgaaaag atttcctttc
      181 agtgaagatt tgatacgttc aagaaaaaaa tccacatgct gaactggttt tatttctttg
      241 agaagtgtcg gtgcttcaag attcttacct acaacgtgaa agatcatttt attaatgact
      301 aaattttgta cgtcttgttc agaaaggaaa ctcatcctga ctccataatc aagcagatta
      361 gaactatgac tagtctagta tatgcatctt ttggtgcgta cgcaaaagtt cttaaactca
      421 aaatgttatc gcggatagat ctttgtagag tcagtgattg gcgcaggtag gtggtaacag
      481 cagacaaata gctgcggata cttgaggcta tctgcctcgg gcatgaacat caaaggcaga
      541 tagagaaaag ccccagttaa cattacgcat ccggcaagac gcttaacatt aatctgaggc
      601 caatttcatg ctttgcacat gtaggttagc ctcttacgtg ccgaaaggca aggagaagca
      661 ggctatgaag cagcaaaagg cgatgttagt cgccctgatc gtcatctgtt taaccgttat
      721 agtgacggta ctggtaacga ggaaagacct ctgcgaggta cgaatccgaa ccgaccagac
      781 ggaggtcgct gtcttcacag cttacgaact tggggagtaa gaggaccggc ggggag
//
LOCUS       DAZUCE010000529          833 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZUCE010000529 DAZUCE010000000
VERSION     DAZUCE010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.532"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>833
                     /locus_tag="ACHY03_005346"
     CDS             <1..>833
                     /locus_tag="ACHY03_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFS3908423.1"
                     /translation="VFNVVGENGQVTEAVLTQDGPVLENFTAGQSGTLSDDDRGGFIW
                     LGGLAFGGGLAAMALAAGGGGGSKHRHENHEADTTAPSAPELTAGDNGSVSVELPGDA
                     NKGDTVEITFEDENGDEQKVTLEKGDNGWTSSNPALIPDSQGDTATIAPDTVKDNSEV
                     TAVAKDPAGNESTPVTVTSKTDGVADAPVLTIPEVADSVANAAELKDGLQAEVKLPAG
                     TVEGAVITLTVTHPDQSTENVTHNVTGDEVTAGKVSMDIPEDAVVDGQNSVSVSLTQG
                     S"
BASE COUNT          227 a          169 c          250 g          187 t
ORIGIN      
        1 gtatttaatg ttgtgggtga aaatggtcag gtaactgaag ctgtcctgac ccaggatggc
       61 cctgtactgg agaatttcac tgcaggacaa tccgggacct tgtcagatga tgatcgtggt
      121 ggttttatat ggttgggcgg tctggcattt ggtggggggc ttgctgctat ggctttggct
      181 gcaggtggtg gtggcggcag caagcacaga catgaaaacc atgaggcaga tacaacagca
      241 ccgtcagcac cggaattaac ggccggagat aatggttcag tttctgttga actgccgggt
      301 gatgcaaata aaggcgatac tgttgaaatc acctttgaag atgaaaatgg tgatgaacag
      361 aaagtcacac tggagaaagg ggataacggc tggacatcca gcaatcctgc actgatcccg
      421 gacagccagg gtgatacagc cacaatcgcc ccagacactg ttaaagataa cagtgaagtc
      481 acagcagttg ctaaagatcc ggctggtaat gaaagtactc cggtcactgt gacgtcaaaa
      541 actgatggtg tggcagatgc tccggtcctg accattccag aagtggctga tagtgtggcc
      601 aatgcggctg agctgaaaga tggtttacag gcagaagtca agttgccggc aggaacagtg
      661 gaaggtgcgg tcattacact gacagtgact catccggatc aaagtaccga aaatgtgaca
      721 cataacgtca ccggagatga ggttactgcc ggtaaggtat ccatggatat tcctgaggat
      781 gctgttgttg acggtcagaa cagtgtgagt gtcagcctga cccagggcag taa
//
LOCUS       DAZUCE010000530          831 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZUCE010000530 DAZUCE010000000
VERSION     DAZUCE010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.533"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>831
                     /locus_tag="ACHY03_005347"
     CDS             <1..>831
                     /locus_tag="ACHY03_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFS3908424.1"
                     /translation="QALHSSWLETLSCWFENKRIIAFCDETQTFSFEKGTNYADLQRI
                     INPQFSFNLSVVLRCPRAVTDYLIAVSPASYQITSPRNNEPDTLKELVVTDPLSTLLN
                     EILQLKESSVLPDEITVLVPTDLVKNSLQNDMRTLGVNIETVSRFRGMESSVVITLYS
                     ESMEDAQLFCAYSRATTAFIAIYDSEKLAWREKNKFLHKLVNRKEVQRAINEAKHNSS
                     SSVLMAGFFREGELKLSTVKLAWSKHFSSWLVEFDERNSPEEMWIDYLYSYLPYPIIY
                     "
BASE COUNT          255 a          140 c          161 g          275 t
ORIGIN      
        1 acaggcgtta cattcttcgt ggctggagac tttatcttgt tggttcgaaa ataaaaggat
       61 tatcgcattt tgtgatgaaa ctcaaacatt ttcctttgaa aaaggaacaa attacgctga
      121 tcttcagcga ataataaatc cccaattttc ttttaactta tctgttgtgt taagatgtcc
      181 tagagccgtt actgattatt taatagctgt cagccctgcc tcataccaaa ttacttcccc
      241 taggaataac gaaccggata cgctaaaaga attagttgtt acagatccac tctccacatt
      301 gcttaatgaa attttacaat tgaaagaaag ttctgtactg cctgatgaga ttactgtcct
      361 tgttcctacg gatttagtga aaaattccct ccagaatgat atgagaactt tgggggtgaa
      421 tattgaaaca gtatctcgtt ttcgaggtat ggaatcatct gtcgtgatta cattgtattc
      481 cgaaagcatg gaggatgcac agctattttg tgcttattca agagctacaa ctgcttttat
      541 tgcaatttat gattcggaga aattagcatg gagagaaaaa aataaatttt tgcataaact
      601 ggttaacaga aaggaggttc agagagcaat taatgaagca aagcataatt cttcctccag
      661 tgttcttatg gccggttttt tccgcgaagg agagttaaaa cttagtactg taaaattagc
      721 atggtcgaag cacttttctt cgtggcttgt tgaatttgat gaaagaaatt ctccagaaga
      781 aatgtggata gattatttgt attcctattt gccttacccg attatatatt g
//
LOCUS       DAZUCE010000531          829 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZUCE010000531 DAZUCE010000000
VERSION     DAZUCE010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.534"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>829
                     /locus_tag="ACHY03_005348"
     CDS             <1..>829
                     /locus_tag="ACHY03_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFS3908425.1"
                     /translation="RKTSKPISTRTLADYEGTYHRHLEKALGNLRIDELTKSVIFEHL
                     SNVRKTSSEAVRKGLIILNMTLDHANLQGMLEHNPARLLKPAMFGASMNKPRERWLPE
                     NELRMLWKALDDATVGGGSIASGGYGISSSVVLSHSVANALRLIIFTGVRRSEAAQMR
                     WDQINGERWTIPATKNGKSHIVTLHPYMLSLIKTQREFTEGAYVFGSTSKPGFPITND
                     ALTRALERVRSKYLAELTPFSPHDLRRSVATGCAEYLDAPERLIELLLNHVPKDRLIR
                     "
BASE COUNT          243 a          182 c          175 g          229 t
ORIGIN      
        1 cagaaagaca agtaaaccaa ttagcaccag aacccttgca gattatgaag ggacttatca
       61 ccgccacctt gaaaaagcat taggaaacct tcgcattgac gaacttacca aaagcgttat
      121 ttttgaacat ctcagtaatg taagaaaaac cagttctgaa gcagttcgta aaggattaat
      181 tattctaaat atgactcttg atcatgccaa cttacagggt atgcttgagc ataacccagc
      241 acgactgctc aaaccagcaa tgtttggtgc atcgatgaat aaacctcgcg agcgatggct
      301 tcctgagaat gaactccgga tgctatggaa ggctttggat gatgctactg ttggaggagg
      361 ctccattgca tctggtggct atggaatttc ctctagtgtg gtattatctc attctgtcgc
      421 aaatgcactc cgtttgatta ttttcacagg agtgcgccgc tctgaagcgg cacaaatgcg
      481 ctgggatcaa attaatggag agcgttggac cataccagcc actaaaaatg ggaaaagcca
      541 tattgttact ttacatccgt atatgctttc tttaataaaa acacaacgtg aattcactga
      601 aggtgcatat gtttttggtt ctactagcaa acctggcttt cccatcacaa atgacgctct
      661 gactcgcgcg ttagaaaggg ttcgctctaa gtatctagcc gaacttactc cattttctcc
      721 tcatgatctg cgtagaagtg ttgcgactgg atgcgctgaa taccttgatg ctccagagcg
      781 cctaatagag ttactgttaa atcatgtacc caaagaccgt ttaatacga
//
LOCUS       DAZUCE010000532          828 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZUCE010000532 DAZUCE010000000
VERSION     DAZUCE010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.535"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..325)
                     /locus_tag="ACHY03_005349"
     CDS             complement(<1..325)
                     /locus_tag="ACHY03_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908426.1"
                     /translation="MKIGTVAGTNDSITTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     NNSYQF"
     gene            complement(587..>828)
                     /locus_tag="ACHY03_005350"
     CDS             complement(587..>828)
                     /locus_tag="ACHY03_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFS3908427.1"
                     /translation="NNTTDFRPARERIFICGETLKCEDPSKHIRQKYGHHGLGVNQNQ
                     KIDDIIMGLNSKMAVFDENKKIIGSSSGLSCFQPR"
BASE COUNT          249 a          166 c          128 g          285 t
ORIGIN      
        1 taaactgata actattattc ctacgtagaa tatgggcacg catgcccgat agcatactat
       61 atttttctga atgatccgtt tgtatggtaa atgatatttt gtcatcttga atagattgtt
      121 ggaaccgttc tatttgccaa tcatctattt tttccacaac ctcaccgagc attgtcctgg
      181 caatacttga tatatcatca cctataccat ttgcaatata cccaaacagt tctttagtga
      241 agttggtaac atgctcttgc accatatcat ttgttgcgat tgtcgttata ctgtcgttag
      301 tacctgccac agttccaatt ttcatctttt acctcatata attcaccgcg aggaatacat
      361 agttatcatt taatcttatg acatacacct catgttcgat ggaaaaataa ttatattttg
      421 cacaaatcat tcagccttaa agcataatat gaaatttctt atctcatagt agagaaagtc
      481 cctaaagatt gtagaggcgt catcagtaca tacctctaca atcgattaag aaaacctatg
      541 aaatactggc aggattaaaa aaacatacca cagcactcat ggagttttaa cgaggttgaa
      601 aacaactaag ccctgaggaa gaacctatta tttttttatt ttcatcaaat actgccatct
      661 tggagtttag ccccatgata atatcatcaa ttttttgatt ctgatttacc cctaacccat
      721 gatggccata tttttgtcta atgtgtttgc tggggtcttc acatttgagt gtctccccac
      781 atatgaagat acgttcacgt gctggtctga aatcagtagt gttattta
//
LOCUS       DAZUCE010000533          826 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZUCE010000533 DAZUCE010000000
VERSION     DAZUCE010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.536"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>826)
                     /locus_tag="ACHY03_005351"
     CDS             complement(<1..>826)
                     /locus_tag="ACHY03_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS3908428.1"
                     /translation="FMFVHLNGLENISLPAGISGFDIEVVLSQSWPADLPVTDDALCL
                     HCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTEIYSVDAVAGSGRTGSAEYVP
                     FTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLILGGQRWEVDRMPERETLSLRI
                     TGTNGQLPRRALQSTLLDRCEQVLQTPVSVCNLCKPTLPVYPPTEDRFHWRVMSHLGT
                     GFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQHHRIQRFEKGFLLRGLDVE"
BASE COUNT          223 a          254 c          184 g          165 t
ORIGIN      
        1 cctccacatc aagcccccgg agcagaaaac ccttttcaaa acgctgaata cgatgatgct
       61 gtacagccag aatggcatca agccgacggt gattaagttc atcgtcccgc cagttataaa
      121 gcgccagcgt gccacgcagc acttccgcac tgctcagcat gttaagaaat cctgttccca
      181 gatgactcat cacccgccag tgaaaacgat cttctgtcgg cggataaacc ggcagcgtgg
      241 gtttacaaag attacataca ctgaccgggg tctgcagtac ctgttcacac cgatcaagca
      301 gcgtactctg caacgcacga cggggaagct gaccattggt acctgtaata cgtaatgaaa
      361 gcgtttcccg ctccggcatc cgatccacct cccaccgctg accgccaagg ataagccatg
      421 tgtcatacat ccccgtgaca ccccgcttca cccgggtatg ataataacgt tcgggtgcat
      481 catggcgcag cattccccca cgatgacgga agctggtaaa aggcacgtac tctgcactgc
      541 cggttcgccc ggagcccgcc accgcatcca ctgaatatat ctccgtatat ccatcctgca
      601 gacgcttcgg gcgcagcaga tattcgctct ccagcccatt gatgatgagt ggatcggctt
      661 caagcgtaaa aaggttaatc accggcacac aatgcagaca cagggcatca tctgtcacag
      721 gtaaatctgc cggccaggac tgactcagca ccacctcaat atcaaatccg gaaatacctg
      781 ccggcaaaga aatattctcc agtccgttca gatgaacaaa cataaa
//
LOCUS       DAZUCE010000534          825 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZUCE010000534 DAZUCE010000000
VERSION     DAZUCE010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.537"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            124..>825
                     /locus_tag="ACHY03_005352"
     CDS             124..>825
                     /locus_tag="ACHY03_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HFS3908429.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAPSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGVWPLLTLSQSQLQRLSPLLDAQSDECPERLQ
                     DAEPVLPQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSR"
BASE COUNT          172 a          221 c          262 g          170 t
ORIGIN      
        1 cttcagtatc tgttggcggg tgctgaagaa agaattctca atcctgaaca cagacctgat
       61 gcgcccgggt tatggcgaag ggcggtagcg tgtctgaagg tcttctgaca gtgagtgaat
      121 aacatggaaa atccggcagc cctgttacgt cgtcttaatc attactgtgc ccgtgcactg
      181 gaaggcgcag cctccctttg ccagacccgg gcccatgcag aaatcacccc ggagcactgg
      241 ttactgaaac tgctggagca gggggaagga gaccttaccg tgctgggccg gcgttatgac
      301 tgggatatgg acgcgatatg gcagtcgctg ctcggctggc tggataacca gccccgtagc
      361 gtacgcagcc gcccgcagct tgcgccgtcc ctgaatgccc tgctgaaaca ggcctggatg
      421 gtggcctcat tacagggaga agaacatatc cgcagcgtgc atctgctggg tgccctgacg
      481 gaaaatccgc acctggttcg ctgtgacggg gtgtggcccc tgctgacact gagtcagagc
      541 cagttgcagc gtctgtcccc gctgctggat gcgcagtctg atgagtgtcc ggagagatta
      601 caggatgcag agcctgtgct gcctcaggga gacaatgtga cctttatcgg gcgtccagtc
      661 ggtgcggata cggcgggtat accgtcaggt gacctgccgc ctgtgttaca gagtgcgctg
      721 gataaattca cccgggacat cacggccagc gcgagagagg ggaaaattga cccggtatcg
      781 ggacgcgata cggaaatccg tcagatggtg gatattctct cccgc
//
LOCUS       DAZUCE010000535          824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZUCE010000535 DAZUCE010000000
VERSION     DAZUCE010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.538"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>824
                     /locus_tag="ACHY03_005353"
     CDS             <1..>824
                     /locus_tag="ACHY03_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFS3908430.1"
                     /translation="GEKGDPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPG
                     PAGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKAATPK
                     AVKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWKTREFVAWL
                     KEKGAFDQPYWMMKASLLASMNKVITDVGPGKLNLGGCVIEVMGTYEAAIVRVTIGEY
                     GATGFINGTVCTCTVYGDTRYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSSDT"
BASE COUNT          226 a          179 c          283 g          136 t
ORIGIN      
        1 aggcgagaaa ggtgatccgg gaggtcctgc aggcccgcaa ggtcctaaag gtgaacgagg
       61 agaagccgga ccacagggtc cgatgggagc acgaggtgag cgtggggaga ctggcccccg
      121 aggtgaacct ggtcctgcag gtccgagagg cgaacgagga gagaccggac ctcagggacc
      181 tcgtggagag ccaggtccgg caggcagcgc tgcaaatgtg gctgatgcga cgacggcaca
      241 gaagggaatt gtgcagttaa gcagcgcaac ggacagtgat gatgaaacga aggcagccac
      301 cccgaaagcg gtgaaagcgg caatggacaa agcggacgga tgcctggaga aagcgaaaaa
      361 cggtgacgat atcccggata aggtgaagtt tctgaacacc gtaggagcag ccagagtata
      421 cgggcgggac attcatacgg gggccggtga atggaaaaca cgcgagtttg ttgcctggct
      481 gaaagaaaaa ggggcatttg accagcctta ctggatgatg aaggcatcac tgcttgcgtc
      541 aatgaataaa gtcatcacag atgtcggacc gggaaaactc aatctgggtg gctgtgtcat
      601 tgaggtgatg ggaacgtatg aggctgccat agtccgggtc accattggcg aatacggtgc
      661 aacggggttt attaatggca cggtctgtac ctgtacggtt tacggcgaca cacggtattt
      721 ccactggcgg gtggattaca gcacaaaaaa caaaccggat acggtcagcc agcgggatgc
      781 cagtacgacg cagaaaggtg tggtgcagtt aagcagtgat actg
//
LOCUS       DAZUCE010000536          824 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZUCE010000536 DAZUCE010000000
VERSION     DAZUCE010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.539"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            123..>824
                     /locus_tag="ACHY03_005354"
     CDS             123..>824
                     /locus_tag="ACHY03_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HFS3908431.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRRRPQLAPSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGLWPLLTLSHSQLQRLSPLLDAQSDECPERLQ
                     DAEPVLPQGDNVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSR"
BASE COUNT          170 a          221 c          261 g          172 t
ORIGIN      
        1 ttcagtatct gttggcgggt gctgaagaaa gaattctcaa tcctgaacac agacctgatg
       61 cgcccgggtt atggcgaagg gcggtagcgt gtctgaaggt cttctgacag tgagtgaata
      121 acatggaaaa tccggcagct ctgttacgcc gtcttaatca ttactgtgcc cgtgcactgg
      181 aaggcgcagc ctccctttgc cagacccggg cccatgcgga aatcaccccg gagcactggt
      241 tactgaaact gctggagcag ggcgaaggag accttaccgt gctgggccgg cgttatgact
      301 gggatatgga cgcgatatgg cagtcgctgc tcggctggct ggataaccag ccccgtagcg
      361 tacgcaggcg cccgcagctt gcgccgtccc tgaatgccct gctgaaacag gcctggatgg
      421 tggcctcatt acagggagaa gaacatatcc gcagcgtgca tctgctgggt gccctgacgg
      481 aaaatccgca cctggttcgc tgtgacgggc tgtggccgct gctgacactg agtcatagcc
      541 agttgcagcg tctgtccccg ctgctggatg cgcagtctga tgagtgtccg gagagattac
      601 aggatgcaga gcctgtgctg cctcagggag acaatgtgac ctttatcggg cgtcccgtcg
      661 gtgcggatac ggcgggtata ccgtcaggtg acctgccgcc tgtgttacag agtgctctgg
      721 ataaattcac ccgggacatc acggccagcg cgagagaggg gaaaattgac ccggtatcgg
      781 gacgcgatac ggaaatccgt cagatggtgg atattctctc ccgc
//
LOCUS       DAZUCE010000537          813 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZUCE010000537 DAZUCE010000000
VERSION     DAZUCE010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.540"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>813)
                     /locus_tag="ACHY03_005355"
     CDS             complement(<1..>813)
                     /locus_tag="ACHY03_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS3908432.1"
                     /translation="LERQKFLFPLDYENKLQHQLDKHFTIESHPLLYRHTMGSKIANV
                     IFEKLYSRIDFNKEQYISFIKDAFLHFYDYSRRYAINENIAKDAVTNNIALMSTFYDS
                     DNTSGEVLNNDFTEESFETALDVEHTIVLGFADDNFETKPVHYQDLLIRFSAFQDTVF
                     NLFPEMRSSHYNDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLVN
                     LKTLLNDGCFIKYSHFNDVALIKQNINNLHLSHTVVNESILKKCCFENGSLG"
BASE COUNT          265 a          152 c          120 g          276 t
ORIGIN      
        1 tcacctaaac tgccgttttc aaagcaacac ttcttcaata tactttcatt cacaacagta
       61 tgtgataaat ggagattgtt tatgttttgc tttattaatg ccacgtcatt aaaatgacta
      121 tatttaataa agcaaccatc attgagtagt gttttaagat tgactaaatt acttgcatct
      181 tcacgtacca tcgtggcaac tggaacaggc agagacatga aaacttcttc attcaccatc
      241 aagtgaatca tgcattgcgt tccctttgtc atatccacac ttacgctaca aatatcgttg
      301 taatgcgaag agcgcatttc agggaacaga ttaaacacgg tatcttgaaa ggcactaaat
      361 cgtattaata aatcctgata atgaacaggt tttgtttcaa aattatcatc agcaaatcca
      421 agcactattg tatgctctac atctaacgca gtttcaaatg actcttctgt aaaatcatta
      481 tttaaaacct cacctgacgt attatcactg tcataaaaag tgctcatcaa tgcgatattg
      541 tttgttacag cgtcttttgc aatattttca ttaattgcat atcgcctgct ataatcataa
      601 aaatgaagaa acgcgtcctt tataaaggat atatattgtt ctttgttaaa gtcaatacga
      661 ctgtataatt tttcaaaaat cacattcgca atcttactac ccatagtatg cctgtataat
      721 aatgggtgcg attcaatcgt aaaatgcttg tctaattgat gttgtaattt attctcatag
      781 tccagtggga aaagaaactt ttgtctttca agt
//
LOCUS       DAZUCE010000538          811 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZUCE010000538 DAZUCE010000000
VERSION     DAZUCE010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.541"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>811)
                     /locus_tag="ACHY03_005356"
     CDS             complement(<1..>811)
                     /locus_tag="ACHY03_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS3908433.1"
                     /translation="KFDNLSPKQKQTTLCCFWNKIASSLPEPYNSTIKHNIIFYKVGE
                     NLMMRGTISIVNEVVKTYSLPIEKDDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGID
                     LGNCNCSNVNFEHTYFDSVKFTNTNCTNANFNSCRFIKCDLTNMNCTGAILDNAVIYG
                     KEKEPEMQYPEADQIIQRITYQKSHGNETKGMILTNCSCVKTTFNWADLSESDCQYVD
                     FSEANLSNTILPDIVRMKGTKLYRTDLFNPILKTEAESTEEKDISPLAKIIL"
BASE COUNT          223 a          141 c          149 g          298 t
ORIGIN      
        1 aagaataatt tttgctaatg ggctaatgtc tttttcttct gttgattctg cttctgtttt
       61 taatatcgga ttaaaaaggt cggtacggta aagtttcgtg cctttcatcc tgacgatgtc
      121 aggaagaata gtgtttgaaa gattagcctc gctaaagtcc acatattggc aatcactttc
      181 tgataaatct gcccaattaa aagtggtttt aacacaggag cagttagtta gaatcatccc
      241 tttcgtttca ttcccatgtg atttctgata agttatcctt tgtattattt gatctgcttc
      301 tggatattgc atctcaggtt ctttttcttt accatatatc accgcattat caagaattgc
      361 gccagtgcaa ttcatatttg ttagatcgca tttgataaat ctacaactgt taaaattcgc
      421 atttgtacaa ttcgtatttg taaacttgac gctatcaaaa taagtatgtt cgaagttgac
      481 atttgagcag ttacagtttc ccagatctat accgaaatct atatcgggat ctttattggg
      541 atctttaccg gaaatattgc tgtgtgctag atatagaccg ctgaaatcgt aatatccatt
      601 gtcatccttt tctattggga gtgaataagt ctttactacc tcgttaacaa tagagatagt
      661 tccacgcatc atcagatttt ctccgacttt ataaaaaata atgttatgtt tgatggtgct
      721 gttgtatggc tctggcaatg aggatgcaat tttattccag aaacaacaaa gcgtggtctg
      781 cttttgtttg ggcgagaggt tatcaaattt c
//
LOCUS       DAZUCE010000539          809 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZUCE010000539 DAZUCE010000000
VERSION     DAZUCE010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.542"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..742)
                     /locus_tag="ACHY03_005357"
     CDS             complement(<1..742)
                     /locus_tag="ACHY03_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908434.1"
                     /translation="MATAKIIDHLSFGVTLIASHERVKQELCNATYSILGAKDSIPID
                     QLVWTKLSYIFGDYHPYDTSFDAAEELIIQKSFFDHMWDISLVEMMNHINYESWEQFD
                     WQKTAEMLNLANKEHTNELRSYQHAYRIEFDGVLSLFNEQLIQIFKEAYKAGYNNDEI
                     NNKKKSKNEKLKQFAQLVRTLHIGASCHAAVRWDQKRQLNGNDLLDFHHAEAALGYCD
                     LFLTEKPLKVQVSQEHLGLRELFSCSVES"
BASE COUNT          244 a          155 c          132 g          278 t
ORIGIN      
        1 atgactcaac agagcatgag aataattctc ttaatcccaa atgttcttga ctcacttgaa
       61 ctttcaaagg tttttcagtc aaaaataaat cacaatatcc taaagctgct tcggcatgat
      121 gaaaatctaa aagatcatta ccattgagct ggcgtttctg atcccatctt actgcagcat
      181 gacaagaggc accaatatgt aaagtacgga ctaattgtgc aaattgttta agtttttcat
      241 tttttgattt tttcttatta ttaatttcat cattgttata tccggccttg taagcctctt
      301 tgaatatctg gattaattgc tcgttgaaaa ggcttaacac tccgtcgaac tcaatacgat
      361 atgcgtgttg atagcttcta agctcattgg tatgctcttt attagccaag ttaagcattt
      421 ctgctgtttt ctgccaatca aattgttccc agctttcata gttgatatgg ttcatcattt
      481 caacgagtga aatatcccac atatgatcaa aaaaagattt ctgaatgatc agttcttcag
      541 ctgcatcgaa agatgtatcg tatggatggt aatcgccgaa aatataactt agtttggtcc
      601 atactaattg atcaattgga atgctgtcct tagctcccaa aatcgaatac gtagcattac
      661 ataattcttg tttgactctc tcatgactag ctataagtgt aactccaaaa ctcagatgat
      721 caataatttt ggctgtggcc atccttgttt cgatatcgtt ctgtttcatt agttctaaaa
      781 aaacagtagc agatataggg caaactacc
//
LOCUS       DAZUCE010000540          802 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZUCE010000540 DAZUCE010000000
VERSION     DAZUCE010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.543"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(216..292)
                     /locus_tag="ACHY03_005358"
     tRNA            complement(216..292)
                     /locus_tag="ACHY03_005358"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(256..258),aa:Arg,seq:tcg)
     gene            complement(300..375)
                     /locus_tag="ACHY03_005359"
     tRNA            complement(300..375)
                     /locus_tag="ACHY03_005359"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(340..342),aa:Ile,seq:cat)
     gene            complement(416..>802)
                     /locus_tag="ACHY03_005360"
     CDS             complement(416..>802)
                     /locus_tag="ACHY03_005360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFS3908435.1"
                     /translation="GELEKPHHQLVSTYSELNRHYTELQSEYKHLRRYFGVTAQVPYT
                     DVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRR
                     ATGVELETERFEQTVREVQDLASQNG"
BASE COUNT          192 a          224 c          185 g          201 t
ORIGIN      
        1 gaggacttgt aactatcatc tccggatcag catgtagttt ttatttttcc ggatgatata
       61 tgccgcaata atacccctgc atacagatgc ctgcaaatat ctacgaagca tccggcgaga
      121 ataaacaagg aagtctgagg ctatcttata tgatagcctg ttgctcaaaa gacaatgatt
      181 cactcatcag aaccaacaac gcataatgca gataatggac cgccatcgag gactcgaacc
      241 ccgcgcagcc agcttcgaag gctggcgctc tatcccgatg agctaatggc ggtatgtgat
      301 ggtggccctt gctggatttg aaccagcgac ctggcgatta tgagtcgctc gctctcacca
      361 ctgagctaaa gggccgggcg caggataata acggtacgta actaatcctg caatatcatc
      421 cgttctgact ggctaaatcc tgaacttccc tgaccgtctg ctcaaaacgt tcagtctcca
      481 gctcaacgcc agttgcacga cgccccagcg ccatcgctgc tttgactgtc gaacccgacc
      541 ccatgaaaaa atctgcaacc aggtcacccg gacgactgct cgcgctgatt atctgctgca
      601 gcatttctgc tggtttttcg cacggatgtt tcccgggata gtactgcacc ggtttatgcg
      661 tccacacatc ggtgtacggc acctgcgccg tcacaccgaa ataccgccgc agatgcttat
      721 attcactctg cagttccgta tagtgtcggt tcagttcact gtatgtgctg accagttggt
      781 gatgtggctt ttccagttct cc
//
LOCUS       DAZUCE010000541          792 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZUCE010000541 DAZUCE010000000
VERSION     DAZUCE010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.544"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            70..249
                     /locus_tag="ACHY03_005361"
     CDS             70..249
                     /locus_tag="ACHY03_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFS3908436.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQNSGNKSGKS"
     gene            335..>792
                     /locus_tag="ACHY03_005362"
     CDS             335..>792
                     /locus_tag="ACHY03_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFS3908437.1"
                     /translation="MNIKTMEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDL"
BASE COUNT          280 a          158 c          188 g          166 t
ORIGIN      
        1 taattttaat gagtgtcgac acacaacact catattaatg aaacaatgca acgcaacggg
       61 agaaataaca tggccgaaca tcgtggtggt tcaggaaatt tcgccgaaga ccgtgagaag
      121 gcatccgacg caggccgtaa aggcggtcag catagcggcg gtaattttaa aaatgatccg
      181 caacgcgcat ctgaagcggg taaaaaaggc ggtcaacaaa acagtggtaa taaatcaggt
      241 aaatcctgat tcaccgttaa ttattaatat cagctgataa agataattgc atctgcgggc
      301 cgtatggctc gcagtgattc cactggagaa aaatatgaat atcaagacca tggaagatgt
      361 atttattcac ctactttcag atacctacag cgcagaaaaa caattaaccc gggcactggc
      421 aaaactcgca agagcaacat caaacgaaaa attaagtcag gcttttcatg cgcacctcga
      481 ggaaacacat ggacagattg aacgtattga tcaagttgtg gaatcagaat cgaatctgaa
      541 aattaagcgc atgaaatgtg tggcaatgga aggtcttatt gaagaagcta atgaggtcat
      601 cgaaagtacc gagaaaaacg aagtgcgtga tgccgcactg attgccgcag cacagaaagt
      661 cgagcattat gagattgcca gttacgggac attagcgacg ctggctgaac aattaggtta
      721 ccgtaaagca gcgaagcttc tgaaagaaac cctggaagaa gaaaaggcca ccgacatcaa
      781 actgactgat ct
//
LOCUS       DAZUCE010000542          780 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZUCE010000542 DAZUCE010000000
VERSION     DAZUCE010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.545"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            382..>780
                     /locus_tag="ACHY03_005363"
     CDS             382..>780
                     /locus_tag="ACHY03_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HFS3908438.1"
                     /translation="MVDNVTVSRVCIQSPSFEPDLDGEKNKSQLFVDDIVAYLKSPSV
                     YSLEKEGPLNHFVNHCSEVELGFYSDGAYSILVSRSKQQPEGMILTVSDADAINIVHI
                     SVSPVLIKFLDDIFTCLHTYPDDESFTKEQI"
BASE COUNT          261 a          116 c          143 g          260 t
ORIGIN      
        1 agacacgaag ctacagtata atactcaaga aatttgtcta ttatgaaatt gtttaaatgt
       61 gaatcgaatc acatttagtt attggctgtg tttatgagag ctgacacata aagaatatta
      121 acaaaaatac gtgaatggat aattttttta atttttatca ttgttgttga aaatgatgat
      181 attgattata gggataccat tacaagaagc tgttcccctc gttgacgtcg cggtttaccc
      241 acctcattac caggttgatg acctgatggg gaaatgacgt aaaaagacaa ataactctat
      301 aaatgagcga aatttgatag ttgatattta aatagtaacc ttattattac tacaactttc
      361 atatgaatga ggagaaagaa aatggttgat aacgtaactg ttagcagagt atgtatacaa
      421 tctccttctt tcgagcctga tttggatgga gaaaaaaata aatctcaatt attcgttgac
      481 gatatagttg cgtatcttaa aagtccttca gtttattcac ttgaaaaaga ggggccgtta
      541 aatcattttg ttaatcattg ttctgaagtt gagttaggtt tctatagcga tggtgcatat
      601 tcaattcttg tctccagatc taagcaacaa cctgaaggta tgattttaac cgtaagcgat
      661 gcagacgcaa tcaatatagt acatatttcc gtatctccag tgcttataaa attcctggat
      721 gatattttta cttgccttca tacgtaccct gatgatgaga gttttacaaa agagcagata
//
LOCUS       DAZUCE010000543          780 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZUCE010000543 DAZUCE010000000
VERSION     DAZUCE010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.546"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            44..640
                     /locus_tag="ACHY03_005364"
     CDS             44..640
                     /locus_tag="ACHY03_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HFS3908439.1"
                     /translation="MLWTENDAENTSQWNGYPLQIGRFRKDKAMPALISGEKSTALVT
                     PPQWRNKAFNGLKDPERNYWAKEQITGSPEENIKAAITYLMMKLSNTKEESTIDQYDS
                     TLYSAIVQKGDLADNIRKERKTTIPNLTKNNPGKNLDKIHPGDILYYQKASMKVIITG
                     WKPITIKNVAMNYNGGGDPKYAIKLQFVYTLLTKNRVL"
     gene            643..>780
                     /locus_tag="ACHY03_005365"
     CDS             643..>780
                     /locus_tag="ACHY03_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908440.1"
                     /translation="MRKITIILLLTFSCTKGLAFDISMSKECSGVDELEFIDCVKNSF
                     IM"
BASE COUNT          301 a          137 c          135 g          207 t
ORIGIN      
        1 cactccatac tttcagccgt tggattggta tataataaaa gccatgctct ggactgaaaa
       61 cgatgcggaa aatacttctc agtggaatgg ttatccatta caaattggtc gttttcggaa
      121 agataaagcg atgccagctt taatctctgg agaaaaatct acagcccttg ttaccccacc
      181 acaatggaga aacaaagcat tcaatgggtt aaaagatcca gaaagaaatt actgggcaaa
      241 agaacaaata actggctctc cagaagagaa tatcaaagca gcaattactt acttaatgat
      301 gaaactttct aatacaaagg aagaatcaac tatcgatcaa tatgactcaa ctctatatag
      361 cgccattgtc caaaaaggcg atttggctga caacatcaga aaagagagaa aaacgaccat
      421 acctaactta acgaaaaaca atcctggaaa aaatctcgat aaaatacacc cgggagatat
      481 tttatattat caaaaagcat ccatgaaagt aataattact ggatggaaac ctatcacaat
      541 aaaaaatgta gcgatgaatt ataatggtgg tggcgatccc aagtatgcaa taaaacttca
      601 gttcgtttat acattactca caaaaaacag agtattatga gaatgcgaaa aataacaatt
      661 attctactgc tgacattctc atgtacaaaa ggtctggcat ttgacatttc catgtctaaa
      721 gaatgtagtg gtgttgatga acttgaattt attgattgtg ttaaaaactc atttattatg
//
LOCUS       DAZUCE010000544          780 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZUCE010000544 DAZUCE010000000
VERSION     DAZUCE010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.547"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            86..649
                     /gene="fimA"
                     /locus_tag="ACHY03_005366"
     CDS             86..649
                     /gene="fimA"
                     /locus_tag="ACHY03_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFS3908441.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          201 a          151 c          189 g          239 t
ORIGIN      
        1 tgattcttgt tttataagca ataacagaat gacttaattg ttaactctct ttattcagct
       61 acttaaatat aaattttgga gaattatgaa actcaaacat gttggtatga ttgtcgtttc
      121 tgtgttggcg atgtcgtctg ctgcggtaag cgcagccgag ggtgatgaat cagtaacgac
      181 cactgttaat ggcggtgtta ttcattttaa aggtgaagtg gtaaatgccg cttgtgcgat
      241 tgattccgaa tcaatgaacc aaacggttga gctgggtcag gttcgttctt ctcgcctggc
      301 taaagcgggt gacctcagct ccgccgttgg cttcaatatc aagctgaatg attgtgatac
      361 caatgtttcc agtaatgcag ctgttgcatt cctgggtact actgtcacca gtaatgacga
      421 tacgttagcg ctgcagagtt cagcggcagg ctctgcccaa aatgtcggta tccaaatttt
      481 ggaccgtacg ggtgaggtat taatacttga tggtgccact tttagcgcta aaactgactt
      541 gattgatggc acgaatatac tgccgttcca ggctcgttat attgcactcg ggcagtccgt
      601 agctggtact gcaaacgcag atgcgacctt caaagttcaa tatctataat tcaatattaa
      661 ggtggctata cgtcatctga aattgaaaca gggtgtgtgt ggtatggcta cgcacacccg
      721 agcaattcac caatgtgaat ctcattatta atgaggagtg gtttgtattt ggagcgctat
//
LOCUS       DAZUCE010000545          778 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZUCE010000545 DAZUCE010000000
VERSION     DAZUCE010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.548"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(166..729)
                     /gene="ipbA"
                     /locus_tag="ACHY03_005367"
                     /gene_synonym="fimX"
     CDS             complement(166..729)
                     /gene="ipbA"
                     /locus_tag="ACHY03_005367"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HFS3908442.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          232 a          152 c          160 g          234 t
ORIGIN      
        1 cactggtatg acctttgtgt ttaatcattt caaatcatgg ggttatttta ctttagcgag
       61 aaatatcaac atgttatgtg ttttatttaa ttgaatgggc tgttttttta tggaagaatt
      121 gaggaaaacg taggaatctg acattttgaa tcagaaggta ctgttttaac gaggcttttt
      181 tttccacacc cctttaaatc gagcagcatt actggctgtg tatctgacag tatgctggat
      241 atttcgatga cccagataat cctgaagtag tcgggtatcg acaccattgt cagccagagc
      301 aaaaccacag gcatgacgta acatatgagg atgtgctttt acagctaatc ctgcacgtct
      361 tccagcctca cgaatgatag aaaaaaaatg ttgtctgctg atagggcggc gtttccggga
      421 gagaaaaagc cagtctcctt caactccgtt ttcaattaac ttcctctgct taagccacaa
      481 cttgatcaaa ttatattcat caggaagaag aggatgtgtt gtcgagaaac catttttaat
      541 acgccgaata ttaagttgtt ttcctgatgc atcaatatcg gataaacgta aatcaagaag
      601 ttcactggcc ctaaaaccgt gaatgaaagc catcataatt agacagcgat ttctttcagg
      661 aaaggacatg ctctgggctg catccatcag cctgtagact tcatcctgtg tgagatattt
      721 tctcgtcata cgccaccgtg tacagttgta aaaaagagag gcacacccag ataagtat
//
LOCUS       DAZUCE010000546          777 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZUCE010000546 DAZUCE010000000
VERSION     DAZUCE010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.549"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..641)
                     /locus_tag="ACHY03_005368"
     CDS             complement(<1..641)
                     /locus_tag="ACHY03_005368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908443.1"
                     /translation="MKTFYEDWPETFVSRLDMLRALDDRGSTRRLYLERTGAIFDALA
                     EEIRTAVAGHPEIDASELDIGPLYRYYKRGEKGDPLADLLIELAPPTCERVRISPEVY
                     TIPYLFFALLIAQGADNDARDFFNMMMRPLIIAYRFKQLARYLGTKGGGRPQHRLKSE
                     AIELADRFFTENPTAPLSRGVQYISGIFVAKYSDPPAASTIRKWLISIYRSDK"
BASE COUNT          218 a          186 c          167 g          206 t
ORIGIN      
        1 tatttatcac tcctgtaaat tgaaattaac cattttctaa tcgtcgacgc tgcaggtggg
       61 tcagagtatt tcgccacaaa aataccggat atgtattgca caccacgcga taatggcgct
      121 gtcgggtttt cagtaaaaaa acgatcagcc agttcaatgg cttcgctttt taatctgtgc
      181 tgtggtcgtc ctccgccctt agtgccaagg taacgcgcca gttgtttgaa acggtaggcg
      241 atgatcaacg gtcgcatcat catattgaaa aaatcgcgag catcgttgtc agcgccttgc
      301 gctatcaaca gtgcaaaaaa cagatacggg attgtgtaca cttcaggaga tatacgaacc
      361 cgttcacagg ttggtggagc taactcaata agtaaatcag ccagcggatc ccccttttct
      421 ccacgtttgt aatagcgata cagcggcccg atatcaagtt cactcgcgtc gatttcagga
      481 tgcccggcaa cggctgtacg tatctcttct gccagagcgt caaatattgc tccggtccgc
      541 tcaagataga gcctccgggt cgagccgcga tcatccagag ctcgtaacat atctaaccgg
      601 ctaacgaaag tttcaggcca gtcctcatag aaggttttca taattacctc gaataataaa
      661 aactgcatta tttgcacaat ttattattcg ttttcacatc gtgaagtcta taaaaaagaa
      721 aacccgccga agcgggttag taaattattt gcgctaagtt ataaggccac accgtaa
//
LOCUS       DAZUCE010000547          776 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZUCE010000547 DAZUCE010000000
VERSION     DAZUCE010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.550"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..259)
                     /locus_tag="ACHY03_005369"
     CDS             complement(<1..259)
                     /locus_tag="ACHY03_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFS3908444.1"
                     /translation="MRFICDNSYFLEGVKEYINPGKFIISGMQTDNVVIISSSRISEI
                     VGFLYKNNISDMNTLYFSSPEALRFLWGVVDSPVYDIRHLTE"
     gene            complement(383..>776)
                     /locus_tag="ACHY03_005370"
     CDS             complement(383..>776)
                     /locus_tag="ACHY03_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFS3908445.1"
                     /translation="RELFDAGKRRLPLPEDSYVILCDRNLERLTYCMFCGRRFLVIPV
                     SSVRCLTDIRQAIRRGAWLFGHTARPLTRTEMVVVFGVIFHEYGCTFLADQLGISMKT
                     VCAHLYNAMEKSGLRGVSVKYLCSTADR"
BASE COUNT          234 a          211 c          156 g          175 t
ORIGIN      
        1 gctcagtaag atgtctgata tcataaaccg gactgtcaac cactccccag agaaaacgga
       61 gcgcttccgg agaagaaaaa taaagggtgt tcatatcact tatattattc ttatacagaa
      121 agccgacaat ttcagatatc cgggaagaag atattatcac cacattatct gtctgcatgc
      181 cgctgataat gaatttaccg ggattaatat actccttcac tccctccaga aaataactgt
      241 tatcacaaat aaaacgcata cccgttccac atgaaattta tcacgcctga ccacataaca
      301 ttaattatcc ggagcaccgg gatgcccgtg acacagcatg cacagaaatt cccgtcgcaa
      361 cagccttcat cagaccggct ttttaccggt ctgcggtgct gcagagatat ttaacactga
      421 cacctcgcag accgcttttc tccatcgcat tgtaaagatg cgcacatacc gtcttcatgc
      481 ttatccccag ctggtctgcc agaaaggtac agccgtactc atggaaaata accccgaaga
      541 ccaccaccat ctcggtacgg gtcagtggtc ttgccgtatg tccgaacagc cacgcaccac
      601 gacggatggc ctgcctgata tctgtcaggc atctcacaga ggaaacagga atgacaagaa
      661 aacgacgccc acagaacata cagtatgtaa gcctttccag attacggtca cagagaatca
      721 cataactgtc ttcgggaagt ggcagacggc gctttccggc atcaaacagt tcacgg
//
LOCUS       DAZUCE010000548          774 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZUCE010000548 DAZUCE010000000
VERSION     DAZUCE010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.551"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..90
                     /locus_tag="ACHY03_005371"
     CDS             <1..90
                     /locus_tag="ACHY03_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFS3908446.1"
                     /translation="GLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            121..>774
                     /gene="atoB"
                     /locus_tag="ACHY03_005372"
     CDS             121..>774
                     /gene="atoB"
                     /locus_tag="ACHY03_005372"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFS3908447.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     "
BASE COUNT          196 a          180 c          215 g          183 t
ORIGIN      
        1 ggactcggtg tccgcgacat catgggctat tgcatcactg ccctgctctt ctccggcgtc
       61 attttcgtca ttggtttaac gctgttctga cggcacccct acagacagaa ggaataaaaa
      121 atgaaaaatt gtgtcatcgt cagtgcggta cgtactgcta tcggtagttt taacggttca
      181 ctcgcttcca ccagcgccat cgacctgggg gcgacagtaa ttaaagccgc cattgaacgt
      241 gcaaaaatcg attcactaca cattgatgaa gtgattatgg gtaacgtgtt gcaagccggg
      301 ttggggcaaa atccggcacg tcaggcactg ttaaaaagcg ggctggcaga aacggtgtgc
      361 ggattcacgg tcaataaagt gtgtggttcg ggtcttaaaa gtgtggcgct tgccgcccag
      421 gcaattcagg caggtcaggc gcaaagcatt gtggcggggg gtatggaaaa tatgagttta
      481 gcgccctact tactcgatgc aaaagcacgc tctggttatc gtcttggaga cggacaggtt
      541 tatgacgtaa tcctgcgcga tggtctgatg tgcgccaccc atggttatca tatggggatt
      601 accgccgaga acgtggccaa agagtacgga attacccgag aaatgcagga tgaactggcg
      661 ctacattcac agcgtaaagc ggcagccgca attgagtccg gtgcttttac agccgaaatc
      721 gtcccggtaa atgttgtcac tcggaagaaa accttcgtct tcagtcaaga cgaa
//
LOCUS       DAZUCE010000549          771 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZUCE010000549 DAZUCE010000000
VERSION     DAZUCE010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.552"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>771
                     /locus_tag="ACHY03_005373"
     CDS             <1..>771
                     /locus_tag="ACHY03_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFS3908448.1"
                     /translation="DVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDE
                     KSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKA
                     FEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFAWKGLKKLCVA
                     VSFRLIIAEQKKEPKMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDD
                     CKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMR"
BASE COUNT          269 a          134 c          187 g          181 t
ORIGIN      
        1 agacgtcatt gcaattgatg gaaaaacgct ccggcactct tatgacaaga gtcgccgcag
       61 gggagcgatt catgtcatta gtgcgttctc aacaatgcac agtctggtca tcggacagat
      121 caagacggat gagaaatcta atgagattac agctatccca gaacttctta acatgctgga
      181 tattaaagga aaaatcatca caactgatgc gatgggttgc cagaaagata ttgcagagaa
      241 gatacaaaaa cagggaggtg attatttatt cgcggtaaaa ggaaaccagg ggcggctaaa
      301 taaagccttt gaggaaaaat ttccgctgaa agaattaaat aatccagagc atgacagtta
      361 cgcaattagt gaaaagagtc acggcagaga agaaatccgt cttcatattg tttgcgatgt
      421 ccctgatgaa cttattgatt tcacgtttgc atggaaagga ctgaagaaat tatgcgtggc
      481 agtctccttt cggttaataa tagcagaaca aaagaaagag ccaaaaatga cggtcagata
      541 ttatatcagt tctgctgatt taaccgcaga gaagttcgcc acagcaatcc gaaaccactg
      601 gcacgtggag aataagctgc actggcgtct ggacgtggta atgaatgaag acgactgcaa
      661 aataagaaga ggaaatgcag cagaattatt ttcagggata cggcacattg ctattaatat
      721 tttgacgaat gataaggtat tcaaggcagg gttaagacgt aagatgcgaa a
//
LOCUS       DAZUCE010000550          765 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZUCE010000550 DAZUCE010000000
VERSION     DAZUCE010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.553"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..678
                     /locus_tag="ACHY03_005374"
     CDS             <1..678
                     /locus_tag="ACHY03_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS3908449.1"
                     /translation="KRAQREREKLRKYDADCHDESRRVTHLSRQVTTDKDTDTELNPT
                     HNARMRESAPTGESNGASLQTAEPEFLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGM
                     ALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAAS
                     GGTASRAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDTEERGRTFEALD
                     CPDWRDD"
     gene            671..765
                     /locus_tag="ACHY03_005375"
                     /pseudo
     CDS             671..765
                     /locus_tag="ACHY03_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT          190 a          191 c          248 g          136 t
ORIGIN      
        1 aagagagcgc agcgggagcg cgaaaagctg cggaaatatg atgctgattg tcacgatgag
       61 tcacgacgtg tcacgcatct gtcacgacaa gtcacgacag ataaagatac agatacagaa
      121 ttaaacccca cacataacgc gcgcatgcgc gagagtgctc caaccggtga gtcgaatggt
      181 gcgtcgttgc agacagccga acctgaattc ctggacggcc tgagcgaacc gatcgggaaa
      241 ttttcgatga ccactgtctg gcagccgtcg ccggattttc gacaacgggc agcagtgtgg
      301 ggtatggctc tgcctgagcc ggaatttaca cctgcagagc ttgccgcatt ccgggattac
      361 tggatggcgg aggggaaggt tttcacgcag gttcagtggg agcagaaatt tgcccgccac
      421 gtgcagcacg tcagggcaca ggtaaaacca gtcagcaagg gggtaagcca tgcagcatca
      481 ggtggcacgg catcacgggc agttcaggaa atccgggcag cacgcgaaca gtgggaacgt
      541 gaaaacggat ttatcagcaa cggaaacggc ctggaagctg tgggagctta tgggggaggt
      601 gtattcgaac cgctggacac agaagaacgg ggccgcacct tcgaagctct ggattgccca
      661 gattggcgcg atgactgaac agcaaatccg gctggtctgc cgtcagtgca tggaccgctg
      721 ccgggcgggt gaaacgtggc ccccggacct ggctgagttt gttgc
//
LOCUS       DAZUCE010000551          759 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZUCE010000551 DAZUCE010000000
VERSION     DAZUCE010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.554"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..672
                     /locus_tag="ACHY03_005376"
     CDS             <1..672
                     /locus_tag="ACHY03_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFS3908450.1"
                     /translation="KRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDTELNPT
                     HNARESIPTSESNGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMAL
                     PEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGG
                     TASRAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCP
                     DWCDD"
     gene            665..759
                     /locus_tag="ACHY03_005377"
                     /pseudo
     CDS             665..759
                     /locus_tag="ACHY03_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
BASE COUNT          189 a          187 c          243 g          140 t
ORIGIN      
        1 aagagagcgc agcgggagcg ggaaaagctg cggaaacata atgctgattg tcacgatgag
       61 tcacgacgtg tcacgcatct gtcacgacaa gtcacgacag ataaagatac agatacagaa
      121 ttaaacccca cacataacgc gcgcgagagt attccgacca gtgagtcgaa tggtgcgccg
      181 ttgcagacag ccgaacctga atacctggac ggcctgagcg aacccatcgg gaaattttcg
      241 atgactactg tctggcagcc gtcgccggat tttcgacaac gggcagcagt gtggggtatg
      301 gctctgcctg agccggaatt tacacctgct gagcttgccg cattccggga ttactggatg
      361 gcggagggga aggttttcac gcaggttcag tgggagcaga aatttgcccg tcacgtgcag
      421 cacgtcaggg cacaggtaaa accagtcagc aagggggtaa gccatgcagc atcaggtggc
      481 acggcatcac gggcagttca ggaaatccgg gcagcacgcg aacagtggga acgtgacaac
      541 ggatttatca gcaatggaaa cggcctggaa gctgtgggag cttatggggg aggtgtattc
      601 gaaccgctgg actcagaaga acggggccgc accttcgaag ctctggattg cccagattgg
      661 tgcgatgact gaacagcaaa tccgtctggt ctgccgtcag tgcatggacc gctgccgggc
      721 gggtgaaacg tggcccccgg acctggctga gtttgttgc
//
LOCUS       DAZUCE010000552          750 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZUCE010000552 DAZUCE010000000
VERSION     DAZUCE010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.555"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..552
                     /locus_tag="ACHY03_005378"
     CDS             <1..552
                     /locus_tag="ACHY03_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HFS3908451.1"
                     /translation="NNVTIAENCVVKGTISADRIIGDIVKGYSFEPRDGTLHITNHKY
                     GEGYVIFKAEVSLADDVSFDRKLILLPFTFYSGTLTIEINGRVVIKADPRVANTGTAG
                     YFDRYDNPVYTRTNLGNTVSGVWDVAAGGGDTRITVTMRVEGGPGNMGDVEGRKRELV
                     RWRYDAFTLMVAKSTDSGFTRRS"
BASE COUNT          209 a          157 c          215 g          169 t
ORIGIN      
        1 aataacgtga ccattgcaga aaactgcgtt gttaaaggaa ccattagcgc agacagaatc
       61 atcggtgata tcgtcaaggg ctattccttc gaacctcgtg atggcacgct ccatattacg
      121 aaccacaagt atggagaggg ttatgtcatc tttaaggcgg aggtatctct tgctgatgat
      181 gtttcgtttg atcgcaagct gatactcctt cccttcactt tctattcagg aacactgacg
      241 atagagatta acggaagagt tgtcattaag gcagacccaa gagttgctaa tacgggaacg
      301 gctggttatt ttgacagata cgacaatcct gtttacacgc gcaccaacct cgggaacacc
      361 gtttcaggag tatgggatgt ggcggcagga gggggagata cacggattac agtgacaatg
      421 agagttgaag gtggtcctgg caatatgggg gatgtggaag ggagaaagag agagctggtg
      481 cgatggagat atgacgcctt tactctcatg gtggcgaaat cgacagattc cggttttacc
      541 cggagatcgt gacaggcagc cggaatacgg attgccggaa ggagacaaaa accgtacatt
      601 atgcgcgggt gcctttggct gatggccgga gggaacacct gaaggccgga tgtgaaaagg
      661 ccccggacaa aacattcatg tttaacccga ggcctgacca ttcatcctaa gcaagtgaaa
      721 ggttagcgcc tctccgtaaa aggggcaagc
//
LOCUS       DAZUCE010000553          739 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZUCE010000553 DAZUCE010000000
VERSION     DAZUCE010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.556"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            74..>739
                     /locus_tag="ACHY03_005379"
     CDS             74..>739
                     /locus_tag="ACHY03_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908452.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPEQYDLARQHLPQIYACRTNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVIFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQN"
BASE COUNT          284 a          149 c          122 g          184 t
ORIGIN      
        1 agaaaatcca ttcctgctct aattatccaa tttaaaaatc ttagcataaa acagatgatg
       61 aataaggaat gttatgtcaa ttgactttac cccaggtatg ataaatacat atcacggcga
      121 tatttataac cgcacaacag ataccgacaa tgttaaaaca ccagacacac cgatgtggcc
      181 ttgtgataat cgggaagaac aacaacccat taattccaca ttctctggag aaggatacaa
      241 ccctgaacaa tatgatttag cgcggcaaca cctcccacaa atttatgcgt gccgcacgaa
      301 caccaaatat acagatgcag actactcaaa agttgtggct caacttgtaa gtcttattac
      361 taacattgaa tcgataagtt cgacaccact tacgcggcag gcacaggaaa tacttaatca
      421 aattaataac attagatacg aaaagaacaa aagtgctgaa tgtcgaatca ttgttgtcgc
      481 taatcctaaa cccgacaagg caatcataac taaaattagc gtagaagagg gaataccagt
      541 aatattcagt gtccaaacca tgttttcaga tactaatttt attgctgaac aacgagctga
      601 cttaccgact aatataaaag atattcaaag cctgtatcaa aaaatgacga agttatatat
      661 tgagcacagt gaaaataaaa acaggatgaa agtctttgcc ggaaccaact ttattgattt
      721 caatatgaca ggacagaac
//
LOCUS       DAZUCE010000554          736 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZUCE010000554 DAZUCE010000000
VERSION     DAZUCE010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.557"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>736
                     /locus_tag="ACHY03_005380"
     CDS             <1..>736
                     /locus_tag="ACHY03_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3908453.1"
                     /translation="GFESSGAETALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVE
                     TTSKGDRNPSSVRLLIQLERNGHWVTEKDVTINGKTTSQYLTSVILNNLPERPFNIRM
                     VRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQLTVNYHIR
                     GRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAPDVD
                     KWALYAIGQYCDQTVPDGFGGTEPRMT"
BASE COUNT          171 a          202 c          233 g          130 t
ORIGIN      
        1 aaggctttga gtcctccggg gcggaaaccg cactgggcgt ggaggtgacg aaggcaaagc
       61 cggtgacgcg caccatcacg tcagcgaaca ttgaccgtct gcgggtcacc ttcggggtac
      121 agtcactggt ggagaccacc tcaaagggtg accgtaatcc ctcttctgtc cgcctgctga
      181 ttcagcttga gcgtaacggg cactgggtga cggagaagga tgtaaccatt aacggcaaga
      241 ccacctcgca gtacctgacg tcggtgattc tgaataatct ccctgagcgc ccctttaaca
      301 tccggatggt cagggagacg gcggacagca ccacggacca gctgcagaac agaacgctgt
      361 ggtcgtcata caccgaaatc atcgatgtga aacagtgcta cccgaacacg gcgattgtgg
      421 ggctgcaggt ggatgcggag cagtttggtg gccagcagct gacggtgaac taccatatcc
      481 gtggtcgcat cattcaggtg ccgtcaaact atgacccgga aaaacgcacc tacagcggta
      541 tctgggacgg gagtctgaaa ccggcataca gcaataaccc ggcctggtgc ctgtgggaca
      601 tgctgaccca cccgcgctac gggatgggaa aacgcctggg ggccccggac gtggacaagt
      661 gggcactgta tgccatcggg cagtactgtg accagacggt cccggatggt ttcgggggaa
      721 cagagccgcg gatgac
//
LOCUS       DAZUCE010000555          734 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZUCE010000555 DAZUCE010000000
VERSION     DAZUCE010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.558"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            45..>734
                     /locus_tag="ACHY03_005381"
     CDS             45..>734
                     /locus_tag="ACHY03_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS3908454.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQK"
BASE COUNT          244 a          129 c          162 g          199 t
ORIGIN      
        1 cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga cgtaaatggg
       61 ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat
      121 tcggtgtaga aatctatgct gctgccaaac aaggtgaacc cgatccagaa ttaaacacat
      181 ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac gttattgata
      241 aagcaattga taaagccaaa ggcggcggag atgaaacatt cgtgcagggc cgttatgaag
      301 gctttggtcc taatggctca atgattatcg ccgagacatt gacttcaaat gttaaccgta
      361 cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt
      421 ctgtcagcta tatgtttgac aatacgggtg tgattgtatt taaaggaaca gaccctgacc
      481 atatttttga aattttactt gaagctgaag ttgatgttcg tgatgtgact gaagaagaag
      541 gtaacattgt tatttatact gaacctactg accttcataa aggaatcgcg gctctaaaag
      601 cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg
      661 agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt gaagatgacg
      721 acgatgtaca aaaa
//
LOCUS       DAZUCE010000556          728 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZUCE010000556 DAZUCE010000000
VERSION     DAZUCE010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.559"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>728)
                     /locus_tag="ACHY03_005382"
     CDS             complement(<1..>728)
                     /locus_tag="ACHY03_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFS3908455.1"
                     /translation="KSQGEGTLPIRHKLEFISTNIAELLDKLTKITDARLCKGFSDWA
                     SSVKEGASNDLKENVDRALVRMFKCVKLHSNELNLSYLFLGSVPPLPEWIEMLSLIHN
                     KLDSIQVPESCKELEVDFNNLTEFPQVPDGITLISVNNNLISHIDSFPPKVKKIFIGH
                     NKLSEIPAIPDTAKVFDCSENNIKEIRWFPKNLKEVHIEYNKIEVIPAIPGNLKLLFM
                     ECNPIKEAFLMPWTLTGICYEISQ"
BASE COUNT          216 a          142 c          124 g          246 t
ORIGIN      
        1 ctgcgatatt tcatagcaaa tccctgtaag agtccatggc attaaaaatg cctctttaat
       61 aggattacat tccataaaaa gtaattttaa attgccaggt atcgcaggaa taacctcaat
      121 cttattatat tcaatatgca cttctttcag atttttgggg aaccacctaa tttcttttat
      181 attattctca ctacaatcaa aaaccttagc ggtgtctggt attgctggta tttccgatag
      241 cttattgtga ccaataaaaa ttttcttaac ctttggcgga aatgagtcaa tatgcgatat
      301 caggttatta tttacggaga tcagggtaat tccatcaggt acttgtggaa attctgtaag
      361 gttattgaaa tcgacttcta attctttgca ggattcgggc acctgtattg aatcaagttt
      421 attatgaata aggctaagca tttcaatcca ctctggaaga ggcggcacag aacccaaaaa
      481 aaggtatgat aagttaagtt cattactgtg aagcttaaca catttaaaca ttctcaccaa
      541 tgctctatcc acattttctt tgaggtcatt cgatgcgcct tctttgactg aactagccca
      601 gtcactgaaa cctttgcaaa gtctggcatc tgtaattttg gttaacttgt ccagcaactc
      661 tgcaatattg gtcgatataa actctaattt atgtcgaatg ggtaaagtcc cctccccctg
      721 actttttt
//
LOCUS       DAZUCE010000557          726 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZUCE010000557 DAZUCE010000000
VERSION     DAZUCE010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.560"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(124..330)
                     /locus_tag="ACHY03_005383"
     CDS             complement(124..330)
                     /locus_tag="ACHY03_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908456.1"
                     /translation="MLNYRISSVQVFTVNGRADRVQVRLECDDAAVFECCLLAEGEGE
                     LKELSLSELEERALMYAADSFRYE"
     gene            complement(458..>726)
                     /locus_tag="ACHY03_005384"
     CDS             complement(458..>726)
                     /locus_tag="ACHY03_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFS3908457.1"
                     /translation="RMNSQQRQINENHEEMKRAAAQSAALAGLFQPYSVGKFNATAAV
                     GGYSDRQAVAVGMGYRFNEQAAAKAGTAFSDGDVSWNVGVNWEF"
BASE COUNT          180 a          244 c          139 g          163 t
ORIGIN      
        1 tcagcatacg catcaggcca tattccagat atctgtcacg ggtcataata ataatctgca
       61 tactctcaga cccttatctc ccggataccg gctccgttca ccattaccgg tataactgac
      121 tcatcattca taacggaaac tgtctgccgc atacatcagt gcccgctctt ccagctctga
      181 cagactcagc tctttcagtt ccccttctcc ttcagcaaga agacaacatt caaaaacagc
      241 tgcatcgtcg cattccagac gaacctgcac cctgtcagcc cgaccgttta cagtaaatac
      301 ctgcactgaa cttatgcgat agttcagcat atacactctc cctctcagct cctcaggaat
      361 accccggaac cgtcagtcat gccaccgggg agaaaagcag gcagcgaatc atgaccgcat
      421 acagaacgct gcccgccccg gtgactcaga cccgggttca gaactcccag ttgacaccca
      481 cattccagga aacatcaccg tcactgaacg cagtacctgc ctttgccgca gcctgctcgt
      541 taaagcgata tcccatgccc accgcaacag cctgcctgtc actgtacccg cccacagccg
      601 ctgtggcgtt aaatttaccc acactgtatg gctggaaaag accggccagc gcagctgact
      661 gggctgctgc ccgcttcatt tcctcatgat tctcattaat ctgacgctgc tgactgttca
      721 tacgac
//
LOCUS       DAZUCE010000558          722 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZUCE010000558 DAZUCE010000000
VERSION     DAZUCE010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.561"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..589
                     /gene="espX1"
                     /locus_tag="ACHY03_005385"
     CDS             <1..589
                     /gene="espX1"
                     /locus_tag="ACHY03_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFS3908458.1"
                     /translation="LPYNKSLEMYELNEQKIQYVTTPNININKLLLSNITIKKSNLSH
                     GYYFGCVLSNISCFESDLSNTIFSNGEINNLFIKKSNIFGTSFTNTMIKNLRCEDIMP
                     GRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKEL
                     KKFMHDYDKTSPEMRKYLQSINESMFLMKKISHQD"
BASE COUNT          281 a          132 c          107 g          202 t
ORIGIN      
        1 attaccctat aataaatcgc tggagatgta tgaacttaac gagcaaaaga tccagtatgt
       61 gactacccct aatattaata taaataaact gttgctatca aatataacaa taaaaaagag
      121 caacctttca catgggtatt attttggttg tgtattatcc aatatatcat gttttgaaag
      181 cgacttatca aacaccatct tttcaaatgg agaaataaac aacctcttca ttaaaaaaag
      241 caacatattt ggaacatcgt tcactaatac catgataaaa aatttgcgct gtgaagatat
      301 tatgcctggc cgatggacca ctcaacttgt caataagcac ttaggttaca gatacacagg
      361 agtattcaaa accctggcaa gtattgatga caaaccgtcc cgtttcgaaa ttttgatacc
      421 attggtacaa actttagttc gcgacaatgt aaagctaaat aatgatgtgt ataaagaact
      481 gaaaaagttt atgcacgatt acgacaaaac atccccagaa atgagaaaat accttcagag
      541 catcaacgag tctatgttcc ttatgaaaaa aatatctcat caagattaaa ataccccaaa
      601 aaagaagtaa tttcagaatg aaattacctg tgataattat taattaactg cagagaaata
      661 aaaaataatc tgaagcatgc tctgaaacat cctactctgt cactctaatg atggcacctt
      721 gc
//
LOCUS       DAZUCE010000559          722 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZUCE010000559 DAZUCE010000000
VERSION     DAZUCE010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.562"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>722
                     /locus_tag="ACHY03_005386"
     CDS             <1..>722
                     /locus_tag="ACHY03_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFS3908459.1"
                     /translation="AIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSVGKTTS
                     EQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMSGFGRVN
                     TMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEGINALAG
                     SVNMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSIGVMAA
                     VSGSSVYSNFSNGSGINSKEFG"
BASE COUNT          194 a          156 c          189 g          183 t
ORIGIN      
        1 gctatcggct caggcatatt attgctcagc tgcggtgctt actcacaatc catcagtgaa
       61 aaaactaatt ccgacaaaaa aggagcggca gaattcagtc cgctcagcgt ttctgtcggg
      121 aagacgacca gtgagcagga agctctcgag aaaacaggcg cgaccagttc ccggacaacg
      181 gacaaaaacc tgcaatcact tgacgcaaca gtgcgtagta tgcccggtac ttatactcaa
      241 atagatcctg gtcagggagc aatcagtgtg aatattcgag gcatgagcgg atttggtcgt
      301 gtaaacacta tggtcgatgg tattacccag agtttttacg gaacctctac ctccggaaca
      361 acgacgcatg gttcaactaa caatatggct ggcgtactta tagatcctaa cttactggta
      421 gcagttgatg ttacacgcgg tgacagcagt ggctctgaag ggatcaacgc ccttgccggt
      481 agtgtaaata tgcgtactat tggcgttgac gatgtaatat ttaacggtaa tacatatggc
      541 cttcgttcac gtttctctgt cggtagtaat gggctgggac gcagcggaat gatcgccctt
      601 ggtggaaaaa gcgacgcttt tacggatacg ggaagcattg gcgttatggc tgctgtgagc
      661 ggcagttctg tgtactctaa tttctcaaat ggttctggaa ttaacagcaa agagtttggt
      721 ta
//
LOCUS       DAZUCE010000560          721 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZUCE010000560 DAZUCE010000000
VERSION     DAZUCE010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.563"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            201..389
                     /gene="dicB"
                     /locus_tag="ACHY03_005387"
     CDS             201..389
                     /gene="dicB"
                     /locus_tag="ACHY03_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFS3908460.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
     gene            386..574
                     /locus_tag="ACHY03_005388"
     CDS             386..574
                     /locus_tag="ACHY03_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFS3908461.1"
                     /translation="MSSAFALMMTVFLITGEPQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSQYLNNEIPAG"
BASE COUNT          227 a          148 c          162 g          184 t
ORIGIN      
        1 ggaaatggat atccgccatt tttaaagtgt attttgtgat gcggtgaatg cggctaagcg
       61 cacgcggaac agttaaaaca agcggtcttt tactggcgta acagacatca actaacaatc
      121 cggcgttaat tgttaactgg ttaacgtcac ctggaggcac caggcaccac atcacaaaat
      181 tcattgttga ggacgcgata atggaaacgt cactaccaaa cgttaatacg tctgaagggt
      241 gttttaatat tggtattctg ctcagtaacc gggagtttac tgaagacgcc atcaggatga
      301 gaaaatatga gccttatctt ctcaatgata attccatact ctccagaatt gcccttcttg
      361 aacttggcat tttcggaggg cagcagtgag ttcagcgttt gcactgatga tgacggtttt
      421 tctgataaca ggtgagccac agaatgtgat taccggaatt tatgccagta aagaatcctg
      481 ccatcaggca agagacgagc aaaaaatttc cggtgaatgt ctcccgttaa acaaagtatc
      541 gcagtacctg aataacgaaa taccggctgg ataacccgcc agccgtatta acgccatacc
      601 cgttgattaa gcatgccagc aatggcaggg attcgtacaa ccttaaaata gttatgaggt
      661 ttatcaatga gcactgataa agaagaaatt gcactgtatt acgaagccaa aaatgacaaa
      721 g
//
LOCUS       DAZUCE010000561          720 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZUCE010000561 DAZUCE010000000
VERSION     DAZUCE010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.564"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>720)
                     /locus_tag="ACHY03_005389"
     CDS             complement(<1..>720)
                     /locus_tag="ACHY03_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFS3908462.1"
                     /translation="LIEQITNLKEWVNKYIADCTDEELNDRDFIASVVDRAIFHFAVN
                     SICNTEGNKDATPIERYTFDVETKNGLPSTVQLFYEESKDNEPLANIHLQAIGSGFLT
                     FVNACQKYDDNSLKLFASLLISLSYSSAYTDLAGAEKVNINDHNGNYLTAQFEELSQR
                     DMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLAPDILSKMRDAAPKLIDFAKSF
                     DPNSKEKIKIYTGTSKLIYDLF"
BASE COUNT          221 a          149 c          111 g          239 t
ORIGIN      
        1 gaataaatca taaattaatt tagaagtacc tgtatatatt ttaatctttt cctttgagtt
       61 tgggtcaaac gacttagcaa agtcaattaa tttcggtgca gcatctcgca ttttgcttaa
      121 aatatcaggg gcgagcgttc cttcattcac cagatgtgac atcttatcca gatagccatc
      181 aaaattcatt tctcccccat ctgccagacg cttcatttct cccaggtact tcttcatatc
      241 acgttgagat aactcttcaa actgagctgt cagatagttc ccattatgat cattaatatt
      301 cactttttct gctcctgcta aatctgtgta ggcactagag tatgaaagtg aaatcaacag
      361 cgaagcgaat aattttaagc tgttgtcatc atatttctga catgcattaa caaacgttaa
      421 aaaaccagaa cctattgctt gcaaatgtat attcgctaaa ggttcattat ctttagattc
      481 ctcataaaat agctgaaccg tggagggaag gccattcttg gtttctacat caaaagtata
      541 tcgttctatg ggcgttgcat ctttattacc ctcagtatta catatactat taaccgcgaa
      601 atgaaaaata gcccggtcaa caactgatgc aataaagtca cgatcgttta attcctcatc
      661 agtgcaatct gcaatatact tattaaccca ctctttgaga ttagttattt gctcaattaa
//
LOCUS       DAZUCE010000562          716 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZUCE010000562 DAZUCE010000000
VERSION     DAZUCE010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.565"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..698
                     /locus_tag="ACHY03_005390"
     CDS             <1..698
                     /locus_tag="ACHY03_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFS3908463.1"
                     /translation="SYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKD
                     EFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGE
                     TKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLI
                     HYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYL
                     KGITSLIKYKLKK"
BASE COUNT          235 a          132 c          137 g          212 t
ORIGIN      
        1 ggtcgtacgc gacatacttc agggtattgt cttttgaata tctgagtgaa agtatttcca
       61 cactgctgta tctggatgcc gatgttgttt gtaaaggaag cctgaaacct ctcacagaaa
      121 ttatatttaa agatgagttt gctgcggtca ttcctgacaa tgatagtact caggcggcat
      181 gtgcaaaacg cctcaacatt cccgaaatga atggacgtta tttcaatgca ggcgttatct
      241 atgtcaatct taaaaaatgg catgaagcaa atttgacacc gtatttactc aaacttttac
      301 gaggggaaac taaatatggc tctcttaaat atttagatca ggatgcgttg aatatcgcat
      361 ttaatatgaa taatatctac cttgcgaagg attttgatac tatttatacc ctgaaaaacg
      421 aactttatga tcgtagtcat cgaaagtatc agcaaaccat taccgataaa acagtgttga
      481 ttcactatac agggataact aaaccatggc atagctgggc tggatatccg tctgcatcat
      541 actttaatat cgcgcgtgaa caatctccct ggaagaaata tcctcttaaa gaggcgcgga
      601 ctgttgcaga aatgcagaaa caatataagc atctgtttgc ccatggtgag tatctaaaag
      661 gcataacttc attaattaag tacaagctta agaaataaat tactcttatg attaca
//
LOCUS       DAZUCE010000563          713 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZUCE010000563 DAZUCE010000000
VERSION     DAZUCE010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.566"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>713
                     /locus_tag="ACHY03_005391"
     CDS             <1..>713
                     /locus_tag="ACHY03_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HFS3908464.1"
                     /translation="VQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGSGENS
                     RLVTTAITADTEHRFSGLPPGEYTLTVRAINSYGRQGEPATTTFRINAPVAPASIELT
                     PGYYQITAVPVLAVYDPTVQYEFWFSEKRITDMAQVETSARYLGTGSQWSVSGPHIKP
                     GKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGMWELIDNSQ
                     LDDEMAEMKTTITETRNEIT"
BASE COUNT          167 a          186 c          231 g          129 t
ORIGIN      
        1 gtgcagcacc tcacggtgga ggtaagtgcc tcagacggcc agtatctggc gctggcgaaa
       61 tgggacacgc cgcgggtggt gaagggcgtg cgcttcagtc tgcgcctgac cagtggcagt
      121 ggtgaaaaca gccgcctggt gaccaccgcc atcactgccg acacggagca ccgtttcagt
      181 ggcctgccgc ccggggaata caccctgacg gtcagggcga taaacagcta cggccggcag
      241 ggcgaacctg ccaccaccac attccggatt aatgcgcctg tagcaccggc cagcattgag
      301 ctgacgccgg ggtattatca gataacagca gtaccggtgc tggcggtgta tgacccgacg
      361 gtacagtatg aattctggtt ctcagaaaaa cgcatcacgg acatggcaca ggtggaaacc
      421 tctgcccgtt atctggggac aggcagccag tggagtgtct ccggcccgca cattaagccg
      481 gggaaggatt tctggtttta tgtgcgcagc gtcaacctgg tggggaaatc tgcgtttgtg
      541 gaagccagtg gccgggccag caatgatgcg gaagggtatc tggggctgtt tcgggaaaag
      601 ataggaaaac tgcatctggc acaggggatg tgggagctga ttgataacag ccagcttgac
      661 gatgagatgg cggagatgaa gaccaccatc accgaaaccc gcaatgaaat cac
//
LOCUS       DAZUCE010000564          711 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZUCE010000564 DAZUCE010000000
VERSION     DAZUCE010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.567"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>711
                     /locus_tag="ACHY03_005392"
     CDS             <1..>711
                     /locus_tag="ACHY03_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFS3908465.1"
                     /translation="EEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIF
                     IAPSPSVQDKPASSDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVS
                     REELQALHIARAEELPAVTGLAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLG
                     LMTAFFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPDLVHTL
                     DTLDVEAAAATLPMDFNIY"
BASE COUNT          192 a          182 c          168 g          169 t
ORIGIN      
        1 gaagaggatt tcaaaaaacc tgttcgcgtg aatttgccca ttgttaatga cctgccgcct
       61 gaaggtgtgt tcgatactga attctgcaac cgctatgaaa aaggcgggga agatggcatc
      121 acaatgatat ttatagcgcc ttccccctca gttcaggaca aaccagccag ctctgacaat
      181 accaacgtca atggcgaaga catggctgag attgaggata atatgctcct gccgatttcc
      241 ggtcaggaac tgcccattcg ctggcttgcg caacatggca gcgaaaaacc ggtaacgcac
      301 gtttcacggg aagaacttca ggcattacat atcgcacgag ctgaagaact accggctgtt
      361 actggcctgg ctatttccca caacacaaag ctgctcgacc cgctggagat tcgcgacctt
      421 cacaaactgg tacgcgacac tgacaaagtt ttccctaatc ccgttaattc cagtctgggg
      481 ttaatgactg cttttttcga agcataccta gacgctgact ataccgatcg aggtctgctg
      541 acaaaagagt ggatgaaagg aaatcgtgtt tctcgcatca cccgcacggc ttccggtgca
      601 aatgccggtg gcggaaataa aaccgatcgt aatcctgact tagtacacac cctcgatact
      661 ctggatgtgg aggctgcagc agccacgctt ccgatggatt ttaatattta t
//
LOCUS       DAZUCE010000565          710 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZUCE010000565 DAZUCE010000000
VERSION     DAZUCE010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.568"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(71..250)
                     /locus_tag="ACHY03_005393"
     CDS             complement(71..250)
                     /locus_tag="ACHY03_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HFS3908466.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
     gene            complement(317..>710)
                     /locus_tag="ACHY03_005394"
     CDS             complement(317..>710)
                     /locus_tag="ACHY03_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908467.1"
                     /translation="FNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
BASE COUNT          188 a          142 c          121 g          259 t
ORIGIN      
        1 gttactcccc gcatatattt tattctcttg ttcgattcta tttttcttaa tcgatcatat
       61 aattttcttg ttagtcggat ttacgaatca tgagacttat aatggaagga atgagactaa
      121 gcggccaggt taaacctgta attattgcag caaacaaccg atatgttact ttttcttccg
      181 agaagataaa tattaaagtc gatgtcacaa ccagacctat caggtaagct aacagtaatt
      241 tattgatcac ttttacctcc cctttcgatt tctctttgag cggtcctttt aagccaggac
      301 cgcctcatta tgaaaattat ttaattccct catcattgat aaggttggac atctctttca
      361 acatgagagt cattgaagct ttcgaccaat ttagcggaat gatagggctt ggcgcttcat
      421 gcaaagttcc cgacttgtgg atatagttat agctttcggg aagtgccatt tctggtacgc
      481 tcctgccatt tgcaccaatc atattctcgc ctgttatttg ggccaatgat ctattcataa
      541 attgtactga gtcattcaaa tactcttctt ttcttgattc tttataaaca attgctgctg
      601 attttgcata ccatgagtca aaaaaccatt ctgcttctgt tgatggaatg aagctctttt
      661 ctctttttgc atgcgaattt tgatcggtat cggttttaat atcattgaac
//
LOCUS       DAZUCE010000566          705 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZUCE010000566 DAZUCE010000000
VERSION     DAZUCE010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.569"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..663
                     /locus_tag="ACHY03_005395"
     CDS             <1..663
                     /locus_tag="ACHY03_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908468.1"
                     /translation="LTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGN
                     RAPETIMQEEMRKYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRF
                     VAAKGHQKAIIVYQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVAT
                     GEWATWSPVNYESRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSR
                     FQ"
BASE COUNT          216 a          143 c          169 g          177 t
ORIGIN      
        1 ctgacagaca aagatttatt cggtaatgaa accactctgg ccgtatccga agaggatatt
       61 caagcagcgc ttgatggaga tgagttccgc gttcccctta attctcctgt aatacttgtt
      121 caatccggca accgcgcacc ggaaaccatt atgcaggagg agatgcgaaa atattatact
      181 gtttccacat tctctggtat cccggacagg caaaagcctc tgacttgtaa caaaaacaaa
      241 gataaaaatg agaacgagga tgttgcccgt gctgagaata tgaactggat gcaggcactg
      301 cgttttgtgg ctgcaaaagg acatcagaaa gcgattattg tttaccagga tatgttgcag
      361 acaggaaaat atgactctgc gctgaaatca acagtatggt ccgactataa aaatgaaaaa
      421 ctcactgacg ctatctccct gcgctacctg gtacgtttca cgctggtgga tgtggcaaca
      481 ggtgagtggg ctacctggtc gccggtgaat tatgaatcga gagtaatttt tccgccggct
      541 ggaataacaa aaacgagcaa taaagaacta tcaaatgacc atgttacgga agcgcaactg
      601 tttaatttga aacaaaaaac atatgtatcg atggtgaaag acttggtgag tcgattccag
      661 taatcgttat tgttatgctg ccgcctgtag tttagggtgg cagca
//
LOCUS       DAZUCE010000567          686 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZUCE010000567 DAZUCE010000000
VERSION     DAZUCE010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.570"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..520)
                     /locus_tag="ACHY03_005396"
     CDS             complement(<1..520)
                     /locus_tag="ACHY03_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS3908469.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDL"
     gene            complement(528..644)
                     /locus_tag="ACHY03_005397"
                     /pseudo
     CDS             complement(528..644)
                     /locus_tag="ACHY03_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
BASE COUNT          182 a          192 c          164 g          148 t
ORIGIN      
        1 gcaaatcgac ctgagaccag tcagccagtt cactgcaggc aaaacctatt ttcagcactg
       61 agcgcccgtc cggctccgta gtcataacga ccttctccac tgccagtgga ttcagtggta
      121 tggcccgggt ggtacggtaa cgacagactg tatttccggg gccaacaggg cgggaagtca
      181 cttccagtcc ggctggtacg gtttcagcca ttttcatgac ggataaccgg ggagtcagtg
      241 ccaccaccga cagggacggg atagtacgca ggtaatgagg ccagagcata ctgaccagac
      301 tttcagtaag ctccggcaaa tcatcatcga ttttctggcg cagccgtccc atggaaaagg
      361 cgaaaccttc aaacaggcgt tccacgcagg gatcaggcgt acccgcttta tcgagatcca
      421 gcatcgccgc ccgatcggga tgagcctgtg caaacgcctt tccggcttca cgcagataac
      481 gcatttcagc gtcaaaataa cgcagggtta aatcgtccat tatcttctca gaataaagct
      541 aactgttccg gagactcgcg ctctgacttc agccatattt caaacaatcg tccgaactga
      601 actgcaggca ggatttcatt aatcagatta cacatgaata taattcagac atctcatgaa
      661 cttcccgaat tgcacaacac agcagc
//
LOCUS       DAZUCE010000568          680 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZUCE010000568 DAZUCE010000000
VERSION     DAZUCE010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.571"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..576
                     /locus_tag="ACHY03_005398"
     CDS             <1..576
                     /locus_tag="ACHY03_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VgrG"
                     /protein_id="HFS3908470.1"
                     /translation="DVINNHAETIGNNQIIAVTNNQIQTVGVNQIETVGSNQIINVGS
                     VQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNV
                     TFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNG
                     DSLLINWNCAASKSPPKTPDEKQDTPDMREY"
BASE COUNT          199 a          140 c          193 g          148 t
ORIGIN      
        1 gatgtgataa ataaccatgc tgagaccatc ggcaacaatc agataattgc ggttaccaac
       61 aatcagatac agacggtggg cgttaaccag atagagacgg tgggcagtaa ccagatcatt
      121 aacgtgggtt ctgttcaggt ggaaacgatt ggacttgttc gtgcgctgac cgtgggcgtg
      181 gcgtatcaga cgacggtagg tggcattatg aacacctcgg tggctctgat gcagtcctcg
      241 cagatcggtt tgcataaatc gttgagggtc gggctgggtt atgacgtcaa agtcggaaat
      301 aacgttacct tcaccgttgg taaaacgaaa aaggatgata ccgggcagac cgcgatttac
      361 tccgccggtg agcatctgga gctctgctgt ggtaaggcaa ggctggtgct gacgaaggac
      421 ggacaaattt ttctcaacgg cacaaaaatt catttgcagg gtaaggagca ggttaatggt
      481 gactcactgt tgattaactg gaactgtgca gcctcgaaat ccccaccgaa gacccctgat
      541 gaaaagcagg atacgccgga tatgagagag tactgatccc gtacctgttc ggtattcagt
      601 aacaaacaag cattaaaaat aaacagaaga aggaattgaa tcatgagcgg aaaaccagcg
      661 gcgcgtcagg gagatatgac
//
LOCUS       DAZUCE010000569          677 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZUCE010000569 DAZUCE010000000
VERSION     DAZUCE010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.572"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..579)
                     /gene="tssG"
                     /locus_tag="ACHY03_005399"
     CDS             complement(<1..579)
                     /gene="tssG"
                     /locus_tag="ACHY03_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFS3908471.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYPGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRT"
     gene            complement(534..>677)
                     /locus_tag="ACHY03_005400"
     CDS             complement(534..>677)
                     /locus_tag="ACHY03_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFS3908472.1"
                     /translation="IHLFGEMLNRFLALYADMNQFNQLTLIVQPEGKCIRWKENHNPR
                     LPG"
BASE COUNT          172 a          167 c          180 g          158 t
ORIGIN      
        1 tgttcttccg ggtaacaaca tcaggggcaa taacgccaga aaacgcgata ccggcgtggc
       61 aatattctgt gcacatccgg ggatccccag tctggccaga ccgagcagat actgggatgt
      121 tttatcctgc ccccccgcct caaaggtggc cgggtacgaa tatttgcgcc agatacggta
      181 atactgggtg atcagacgat gactgaatat atcgagaaaa tccgctgcgg cttcatggcc
      241 ttcccgctgc tgtgcaatat cactgatata gtgagccggc aacggagacg tgaccccgta
      301 aagtcccata aacgtcacat gtacagtggg tggcagatgg gattcttcag gaattacagc
      361 atcttttatt tcactcgcag gaaaccccat tcccgggtat ggacaaaagc gaaccgcttc
      421 ctgacgggcc tgccagccac tgccaatggg aggctgatct ggctggcttt gttccagtaa
      481 ctgacagaag cggtaaaaat taatatacgg aagctgtttc cggtaacggg taattaaccg
      541 ggcaggcgcg gattgtgatt ttctttccac cggatgcatt ttccttcagg ctgaacaatc
      601 agtgtgagct gattgaactg attcatatcg gcgtacaggg ccaggaaacg gttgagcatt
      661 tcgccaaaca ggtgaat
//
LOCUS       DAZUCE010000570          675 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZUCE010000570 DAZUCE010000000
VERSION     DAZUCE010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.573"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>675
                     /locus_tag="ACHY03_005401"
     CDS             <1..>675
                     /locus_tag="ACHY03_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFS3908473.1"
                     /translation="TGLELGPDSDENTGGQWINAGGTGRNTTVTANGRQIVQAGGTAS
                     DTVIRDGGGQSLNGLAVNTTLDNRGEQWVHGGGKAAGTIINQDGYQTIKHGGLATGTI
                     VNTGAEGGPESENVSSGQMVGGTAESTTINKNGRQVIWSSGMARDTLIYAGGDQTVHG
                     EAHNTRLEGGNQYVHNGGTATETLINRDGWQVIKEGGTAAHTTINQKGKLQVNAGGKA
                     SDVTQNT"
BASE COUNT          188 a          161 c          223 g          103 t
ORIGIN      
        1 accgggcttg agctggggcc ggacagtgac gaaaacaccg gcgggcaatg gataaacgcg
       61 ggtggcacag gcagaaacac cactgtcacc gcaaatggtc gtcagattgt gcaggcagga
      121 ggaactgcca gtgatacggt tattcgtgat ggcggagggc agagccttaa cggactggcg
      181 gtgaacacca cgctggataa cagaggtgag cagtgggtac acgggggagg gaaagctgcc
      241 ggtacaatta ttaaccagga tggttaccag accataaaac atggcggact ggcaaccgga
      301 accatcgtca acaccggtgc agaaggtggt ccggagtctg aaaatgtgtc cagcggtcag
      361 atggtcggag ggacggctga atccaccacc atcaacaaaa atggccggca ggttatctgg
      421 tcttcgggga tggcacggga caccctcatt tacgccggtg gtgaccagac ggtacacgga
      481 gaggcacata acacccggct ggaggggggt aaccagtatg tacacaacgg tggcacggca
      541 acagagacgc tgataaaccg tgatggctgg caggtgatta aggaaggagg aactgccgcg
      601 cataccacca tcaaccagaa aggaaagctg caggtgaatg ccggcggtaa agcgtctgat
      661 gtcacccaga acacg
//
LOCUS       DAZUCE010000571          673 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZUCE010000571 DAZUCE010000000
VERSION     DAZUCE010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.574"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            32..562
                     /gene="yodB"
                     /locus_tag="ACHY03_005402"
     CDS             32..562
                     /gene="yodB"
                     /locus_tag="ACHY03_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFS3908474.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
BASE COUNT          195 a          155 c          132 g          191 t
ORIGIN      
        1 cttgactgtg aaaattacag gagctacaaa aatgaaccga ttctcaaaaa ctcaaattta
       61 tttacattgg ataacgctgc ttttcgttgc aataacctat gccgcgatgg aactccgtgg
      121 ctggtttcct aaaggtagta gtacttatct gctgatgcga gaaacacatt acaatgcggg
      181 tatattcgtt tgggtgttaa tgttttcacg cctgattata aaacaccgtt atagtgatcc
      241 ttctattgtg ccaccgccac ctgcctggca aatgaaagcg gcttcgctaa tgcacatcat
      301 gctttatata accttccttg cacttcctct gctggggatt gctttgatgg cttacagtgg
      361 aaaatcgtgg agtttccttg gtttcaatgt gtctcccttt gttaccccaa acagcgaaat
      421 taaagcactg ataaaaaata ttcacgaaac ctgggcaaat ataggctact ttttaatcgc
      481 agctcacgct ggcgcagcac tctttcatca ctacattcag aaagataata ctctgttacg
      541 aatgatgcct cgccgcaaat aagtcgatcc cctgacaagg aaatgtgaac catggacatc
      601 aacgcgtcat aaatgtttac gcaagtgcac aatgcaccct ttccactatc aacaaggagg
      661 agcagaaact gtg
//
LOCUS       DAZUCE010000572          667 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZUCE010000572 DAZUCE010000000
VERSION     DAZUCE010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.575"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..558)
                     /gene="fimE"
                     /locus_tag="ACHY03_005403"
     CDS             complement(<1..558)
                     /gene="fimE"
                     /locus_tag="ACHY03_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFS3908475.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERN"
BASE COUNT          200 a          170 c          138 g          159 t
ORIGIN      
        1 atttctttcc cataatccgg caaaacgagc agcattactg gcggtataac gtacagtatg
       61 gcgaatattt cgatgcccga gataatcctg aattaaacga gtatctgcac cacgctccgc
      121 cagttcataa ccgcaagcat gccttaacat atgaggatga gtctgcgtta cggttccagc
      181 ttcaataccg gcatcacgaa taatgcgata ggcctgctgg cgagaaagcc gactcccgcg
      241 gcgagaaata aatatggcgt cggtccggtc agcgcctttc cagttagcac gttccagggt
      301 ccagcgttcc acggcttcac gctcatcaaa acgtaacggg tgaacggtag aaaatccgtt
      361 cttcagtcgg cgaatattta ttctaccttc attaaggtca agatcctgat aatgcagatc
      421 aagcagttca ctaatacgca tcccatgccg atatgccaac agaataagac aataatctct
      481 ggctcccgtt gccccgtaac aaaccgcctg catcatggcc tgaacttctt taccggtaag
      541 ataacgacgt ttactcacaa tagtagtact cctgactgag atatatttga atgaatacct
      601 ataggaaacc tcaatcggtc aaaattagcc caatagataa aaagataacc acacacgaag
      661 tgatgtg
//
LOCUS       DAZUCE010000573          664 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZUCE010000573 DAZUCE010000000
VERSION     DAZUCE010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.576"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>664
                     /locus_tag="ACHY03_005404"
     CDS             <1..>664
                     /locus_tag="ACHY03_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFS3908476.1"
                     /translation="STSEIIECASPEIRSSVLNDLYSIANFLPDNNLTPNESWKRFCD
                     TCERFYVAQKSITGDKSERLTRKLSISDAGITMTFTIGDVVINTISTAIPEDSTGQRC
                     IEGLNLAEMDLTGIDLSKMVLRNVNFNGSILRKADFFGTICEGVDFTDCDLRYVTFID
                     ASLEKIDFRKARHLLNVNFTNANLRNCNFSGKILTGVNFTGSDLSNAYLAYIDFTKVI
                     FFP"
BASE COUNT          196 a          128 c          135 g          205 t
ORIGIN      
        1 tccacctctg aaattattga gtgcgcctct cctgaaataa gaagtagcgt cctgaacgat
       61 ctttatagca ttgctaattt cctcccggac aataatctga ccccaaatga gagctggaaa
      121 agattttgtg acacatgcga gcgcttttac gttgctcaga agagtatcac tggagataag
      181 agtgaacgtc ttacgcgaaa actctctatc tctgatgcag gaattacaat gaccttcacg
      241 ataggtgatg ttgtcatcaa tactattagc actgctattc cagaagattc aacggggcaa
      301 cggtgtatcg aagggttgaa tttagcagaa atggatttaa ccggtataga cttgtctaaa
      361 atggttctaa ggaatgtcaa ttttaatggc agcattctta gaaaagcgga tttcttcggt
      421 acgatctgtg aaggcgtgga ttttactgat tgtgacctcc gttatgtaac ctttattgac
      481 gcctcattag aaaagatcga ttttcgtaaa gctcgccact tgcttaatgt aaattttaca
      541 aatgcaaatc tacgaaactg taacttcagc ggtaaaattc tcactggcgt taattttact
      601 ggaagtgacc ttagtaacgc ttatcttgca tacatagatt tcacaaaagt gattttcttt
      661 ccgt
//
LOCUS       DAZUCE010000574          663 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZUCE010000574 DAZUCE010000000
VERSION     DAZUCE010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.577"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>663
                     /locus_tag="ACHY03_005405"
     rRNA            <1..>663
                     /locus_tag="ACHY03_005405"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          154 a          146 c          222 g          141 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ccctttaagg gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 agg
//
LOCUS       DAZUCE010000575          661 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZUCE010000575 DAZUCE010000000
VERSION     DAZUCE010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.578"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>661
                     /gene="espX1"
                     /locus_tag="ACHY03_005406"
     CDS             <1..>661
                     /gene="espX1"
                     /locus_tag="ACHY03_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFS3908477.1"
                     /translation="LILFDDFFRSQDLPKPSPVLHHFFQYTHLRDAHFYRCKLIEHTV
                     QFSFFKHKGITLLRLDVFDDRTSECLSEEIKIYQECYEKFIKFLKANFNQEIYPELYT
                     PGIFYEACRNLQSFYAHQETSQNAKYSALDKKKSHLNQEIKKLIKKNIFPELYNEQCN
                     KIPSSSTDDNQQITWQNFKTSNSAYSQLCEKLSLFKSVPSRLIEKSAYCSTENMITNK
                     F"
BASE COUNT          248 a          122 c           93 g          198 t
ORIGIN      
        1 tcttaatttt gtttgatgac tttttcagaa gccaggattt gcctaaacca tctccagtcc
       61 ttcatcattt ttttcaatat actcatctga gagatgcaca tttttataga tgtaaactca
      121 tagaacatac agttcaattt tcttttttta aacacaaagg catcacatta cttagattgg
      181 atgtttttga tgacagaaca agcgaatgtc tttctgaaga gattaagata tatcaagaat
      241 gctatgaaaa atttattaaa tttttaaagg caaacttcaa ccaggaaata tacccagagc
      301 tatacactcc tggaatattt tatgaggcct gcagaaatct ccagtcattt tatgctcatc
      361 aggagacgtc acaaaacgct aaatatagtg cactagacaa gaaaaaaagt catcttaacc
      421 aggaaatcaa aaagctaatt aaaaaaaata ttttccctga gctatacaat gagcaatgca
      481 ataaaatccc ctcctcatcg acagatgata accagcaaat aacatggcag aattttaaaa
      541 ctagcaattc ggcatattca caactatgtg aaaaattatc tttatttaaa agcgtgccca
      601 gtcggttaat agaaaaatca gcttactgct caactgaaaa catgatcacc aataaatttg
      661 a
//
LOCUS       DAZUCE010000576          658 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZUCE010000576 DAZUCE010000000
VERSION     DAZUCE010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.579"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>658
                     /locus_tag="ACHY03_005407"
     CDS             <1..>658
                     /locus_tag="ACHY03_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908478.1"
                     /translation="NGLILMKLPETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKN
                     ARILGDNNARNGADRLVSGTEIQTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEV
                     PRDQYAGAVETMRNKIREGKVPGVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTY
                     DIAEGSVVSLAAGGISFALTASVFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQ
                     L"
BASE COUNT          169 a          172 c          183 g          134 t
ORIGIN      
        1 aatggtctga ttctcatgaa actgccggaa acctggagta caaatgagaa aatgtttgct
       61 tccggagggc agggacatgg gtttgccgct gaacggggaa accatattgt cgacagagtc
      121 cgtctgaaaa acgcacggat cctcggtgat aataatgcca gaaatggagc agacagactg
      181 gtcagcggaa cagaaatcca gacgaaatat tgttcaactg cagcccgtag cgtcggtgcg
      241 gcattcgacg gacaaaacgg acagtatcgt tacatgggaa ataatggccc catgcaactg
      301 gaagtccccc gtgatcagta tgccggcgct gttgaaacca tgaggaataa gatccgcgaa
      361 ggtaaagtac ccggtgtaac cgatcccgca gaagcgtccc ggctgattcg tcggggacat
      421 ctgacttata ctcaggcccg taatatcacc cggttcggga ccatcgaatc ggtcacttat
      481 gatattgccg agggttcggt tgtcagtctg gcggccggag ggatcagttt tgccctgacg
      541 gcatcggtct tctggctcag caccggcgat cgcgatgctg ccctgcagac agctgctgtc
      601 caggcaggaa aaaccttcac ccgcacactg gctgtctacg tcacaaccca gcaactcc
//
LOCUS       DAZUCE010000577          658 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZUCE010000577 DAZUCE010000000
VERSION     DAZUCE010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.580"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>658
                     /locus_tag="ACHY03_005408"
     CDS             <1..>658
                     /locus_tag="ACHY03_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908479.1"
                     /translation="QGIIMLSGITDTRIDKYSNNEKVTLKSDYSQNECAIYYNSSLML
                     ENTVDLRVSWKDGSNLKLLLPKPVSGGRFVTNDGSVHFDGVASIAHLHGIDAELLTIS
                     CAGRGYLNIELLDENPVAEKFRYLHADLPLLSGRNDKLQQISLYENYNLLNAMLACAW
                     NSNSTLCVDFYSDRFGKDKATLNIKRYDGSFIEHDQGLLVDIKNSVVFPANRIDELVV
                     "
BASE COUNT          211 a          102 c          129 g          216 t
ORIGIN      
        1 agcaaggcat tattatgctt tctggtataa cggatactag aattgataaa tattcaaaca
       61 atgaaaaagt aacacttaag tccgattatt cacaaaacga gtgtgctatt tattataatt
      121 catcgctgat gctggaaaat accgttgatt tacgggtctc ctggaaagat ggttctaatc
      181 ttaaactatt attacctaag ccggttagcg gtggccgatt tgtaactaat gatggttctg
      241 ttcattttga tggtgtggca tctatagcac atttgcatgg aatagatgct gagttattaa
      301 ccatatcatg tgctggaaga ggatatctta atatcgagtt attggatgaa aatccagtag
      361 ctgaaaaatt ccgctattta catgccgacc ttcctctttt atcaggacgc aatgacaaat
      421 tacaacaaat ttcactttat gagaactata atttgctaaa tgctatgctg gcatgtgcat
      481 ggaacagcaa tagtacatta tgtgttgatt tttactctga cagatttgga aaggataaag
      541 caacactcaa tattaaacgc tacgatggta gttttattga acacgatcaa gggttactgg
      601 ttgatataaa aaattctgtt gtttttcctg caaatagaat agacgaactg gttgttga
//
LOCUS       DAZUCE010000578          650 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZUCE010000578 DAZUCE010000000
VERSION     DAZUCE010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.581"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            76..>650
                     /locus_tag="ACHY03_005409"
     CDS             76..>650
                     /locus_tag="ACHY03_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908480.1"
                     /translation="MHRSSNLLFLSLMLFSVTGCSFNQLPGTIISRSPDKKLSPPGDT
                     KWVNIEIKNPSPYTKPFPLSVRYISYECQKKRISGFDGSVVSEPGYNVKEIPMQQERG
                     DIWKAKVAMTGGGSCNWTLSAVTLGIEYTDATHLGKGLVPGSAVGVRLAFDGYASRNG
                     QFDFIGGNQFDYYPKYYPFIKKWSDSSQGLM"
BASE COUNT          191 a          112 c          148 g          199 t
ORIGIN      
        1 catctacatt atccatgcct tccggtaacg atacactttt acgatttgct aagtatagtg
       61 aagaggtaat ggaaaatgca tagaagctca aacttgctgt ttctttcctt gatgttattt
      121 tctgttacag ggtgtagttt taatcaactt cccggtacaa taattagccg gtcgcctgat
      181 aaaaagctat cacctccggg agatacaaaa tgggttaata tcgaaataaa aaatccatca
      241 ccttatacaa aacctttccc gttaagcgtc agatatatct cttatgaatg tcagaaaaag
      301 cgaataagtg gatttgatgg ctctgttgtt tcggaacccg gctataatgt taaggaaatt
      361 ccgatgcagc aagagagagg agatatctgg aaagcgaaag tagcgatgac tggtggtggt
      421 tcatgcaatt ggacattaag tgccgttacg ctaggtattg aatataccga tgccactcat
      481 cttggtaaag gtcttgtgcc gggtagtgca gttggtgtga ggctcgcttt tgatggttat
      541 gcttcacgaa atgggcaatt tgattttatt ggtgggaatc agtttgatta ctatcctaaa
      601 tactatccgt tcataaagaa atggagcgat agttcacagg gtttaatgca
//
LOCUS       DAZUCE010000579          647 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZUCE010000579 DAZUCE010000000
VERSION     DAZUCE010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.582"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>647)
                     /locus_tag="ACHY03_005410"
     CDS             complement(<1..>647)
                     /locus_tag="ACHY03_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFS3908481.1"
                     /translation="VLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRF
                     TQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVY
                     DPTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHNYYFYVRSVNTVG
                     KSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSIT"
BASE COUNT          141 a          203 c          166 g          137 t
ORIGIN      
        1 gtaatggatg tcctgatttc agccaggtca ggcgcaagct gaccgttatc aatctgcgtc
       61 cacagctcct gagccagatg ggttttccct atctcgcctt tgaaaaaatc cagatagccg
      121 gatgcgtcat cactcggctg accaacagcc tccacgaatg ccgatttgcc aacggtgttc
      181 acactgcgaa cgtaaaaata ataattatgg cccggtttga tattgatact ggcggctatc
      241 cagtacaacg ccgtaccaag atagcgtgct gtggtttcaa cctgcctgat atccgcaatc
      301 cgcttttccg agaaccagaa ctcaaactgt accgtcggat cataaaccgc aagatgcggc
      361 gtggcggtta tctgaaaata gcccggcgtc agctcaatcc gcgacggcgc tgccggtgcg
      421 gcaatccgga acgataccga cgccggatcg ccctgctgcc cccacgcatt taccgcccgg
      481 actgtcagcc tgtagttccc cagagccagt tgtgtgaagc ggtaagtggt ttccgtcgtc
      541 cgggccgtgc tgaccagccg ctcactgccg tcatccgctg ccacggtcag gcgaagcagg
      601 aagctcacgc ccttcaccac cttcggcgtg tcccagcggg ccagcac
//
LOCUS       DAZUCE010000580          639 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZUCE010000580 DAZUCE010000000
VERSION     DAZUCE010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.583"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>639
                     /locus_tag="ACHY03_005411"
     CDS             <1..>639
                     /locus_tag="ACHY03_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFS3908482.1"
                     /translation="LNFNQQQGLLDKDNKCYILLSSDNSGRVMRLSQQALISMLEPEV
                     KKKTIWNNYSIYPSLQDTHEIVIDDPETICMRAFPLFAKGWEYAQKNKKHQLILNALG
                     FKGYIRDIFMSAIMRKTDFVPECNNQPTELNSSFSSLINDSDQWQQHTLKDKHYASLL
                     TMLDLNEASERDKSKIFFCLSAVFANISHSSVFNGIPDASKTLKRYAFALLV"
BASE COUNT          214 a          111 c          113 g          201 t
ORIGIN      
        1 aattgaattt caatcagcag caaggattgt tagataaaga taataaatgt tatatcttgt
       61 tatcatccga taactctggg cgagtaatgc gcttatcgca acaggcatta atctcaatgc
      121 ttgagccaga ggttaagaaa aagactatct ggaataatta ctctatttat ccatctttgc
      181 aggataccca tgaaattgtc atagatgatc cagaaacgat ttgtatgcgt gcatttcctt
      241 tatttgcaaa aggatgggaa tatgcgcaga aaaataagaa acatcaattg atcctcaatg
      301 ctcttggatt caaaggttat attcgtgata tatttatgtc agcaataatg cgaaaaacag
      361 attttgtgcc cgaatgcaat aatcaaccaa cagaacttaa ttcatcattt tcctctttaa
      421 taaacgattc agatcaatgg caacagcata ctctgaaaga taaacattac gctagtctat
      481 taacgatgct ggatctcaac gaggccagtg aaagggataa gtctaaaata tttttctgcc
      541 tgtctgccgt atttgcaaat atttctcaca gtagtgtctt taacggtatt cctgatgcgt
      601 caaaaacttt aaaacgttat gcatttgctt tattggtga
//
LOCUS       DAZUCE010000581          636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZUCE010000581 DAZUCE010000000
VERSION     DAZUCE010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.584"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>636
                     /locus_tag="ACHY03_005412"
     CDS             <1..>636
                     /locus_tag="ACHY03_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFS3908483.1"
                     /translation="TIAATTWSEYKQYLERDAALERRFQMVRVDEPDDETACLMLRSL
                     KSRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQ
                     LTRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYAREC
                     ELTGELLESRSDISRQSETLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADW"
BASE COUNT          140 a          176 c          187 g          133 t
ORIGIN      
        1 accattgccg ccaccacctg gtctgagtac aaacaatacc tggaacgtga cgcggctctg
       61 gagcggcgtt ttcagatggt cagagtggac gagccggatg atgagacggc ctgtctgatg
      121 ctgcgctccc tgaaatcccg ttatgcggaa catcataacg tgcatatcac cgatgaggcg
      181 gtacgtgctg ccgtcacgct gtcgcgccgt tatctgacag aacgccagtt accggacaag
      241 gccgttgacc tgctcgatac tgctgctgcc cgtgtgcgta tgagcctcga cacggtgccg
      301 gaacagctga cccgtatccg ttcacagctc gcttccctcg gtatggaaaa acaggcactg
      361 ctggaagata ttgccgttgg tcatcagaat cacggcgaac gtctgtctgc cattgagcag
      421 gaagagaatg tgctgatgac agcacgtgat gacctggagc agcagtacgc ccgggagtgc
      481 gaactcaccg gtgagttact tgaaagtcgc agcgatattt cccgtcagag tgagacactg
      541 catctgcagc aggcactgca cgacattcag cagaatcagc ccctgctcag tgtggatgtt
      601 gacgtacgca ccgttgccgg tgtggtcgcg gactgg
//
LOCUS       DAZUCE010000582          636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZUCE010000582 DAZUCE010000000
VERSION     DAZUCE010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.585"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>636
                     /locus_tag="ACHY03_005413"
     CDS             <1..>636
                     /locus_tag="ACHY03_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFS3908484.1"
                     /translation="TIAATTWSEYRQYLERDAALERRFQMVRVDEPDDETACLMLRSL
                     KSRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQ
                     LTRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYARER
                     ELTGELLESRSDISRQSETLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADW"
BASE COUNT          141 a          178 c          188 g          129 t
ORIGIN      
        1 accattgccg ccaccacctg gtctgagtac agacaatacc tggaacgtga cgcagctctg
       61 gagcggcgtt ttcagatggt cagagtggac gagccggatg atgagacggc ctgtctgatg
      121 ctgcgctccc tgaaatcccg ttatgcggaa catcataacg tgcatatcac cgatgaggcg
      181 gtacgtgctg ccgtcacgct gtcgcgccgt tatctgacag aacgtcagtt accggacaag
      241 gccgttgacc tgcttgatac cgctgccgcc cgtgtgcgga tgagcctcga tacggtgccg
      301 gaacagctga cccgtatccg ttcacagctc gcttccctcg gtatggaaaa acaggcactg
      361 ctggaagata ttgccgttgg tcatcagaat cacggcgaac gcctgtctgc cattgagcag
      421 gaagagaacg tgctgatgac agcacgtgat gacctggagc agcagtacgc ccgggagcgc
      481 gaactcacag gcgagttact tgaaagccgc agcgatattt cccgtcagag tgagacactg
      541 catctgcagc aggcactgca cgacattcag cagaatcagc ccctgctcag tgtggatgtt
      601 gacgtacgca ccgttgccgg tgtggttgcg gactgg
//
LOCUS       DAZUCE010000583          636 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZUCE010000583 DAZUCE010000000
VERSION     DAZUCE010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.586"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..250)
                     /locus_tag="ACHY03_005414"
     CDS             complement(<1..250)
                     /locus_tag="ACHY03_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908485.1"
                     /translation="MNVRNVKFDNLSFYEEKIRWRMRRQISNAEAIFYENQEEHIQRF
                     LGRYTCRKPVLVYWESEQRWTSVNGLEVMSVFDEHCHHI"
     gene            complement(237..575)
                     /locus_tag="ACHY03_005415"
     CDS             complement(237..575)
                     /locus_tag="ACHY03_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908486.1"
                     /translation="MKNVENIIWHDGVLVETRMECHHTEYNFILTAELYVDEFTAGRE
                     RKRFEFTGVEHLSQVMDIAELIDNQNAGNIKDATIVYKAKKYKISLHLFGGFLSFTFK
                     TMRVIDNECA"
BASE COUNT          191 a          141 c           94 g          210 t
ORIGIN      
        1 agatgtgatg acaatgttca tcaaacacac tcatcacttc cagaccgttt acagatgtcc
       61 atctttgttc actttcccaa taaacaagaa ccggtttgcg acaggtatat ctgcctaaaa
      121 agcgctgaat gtgttcttcc tgattctcgt aaaatattgc ctctgcattc gagatttgcc
      181 gacgcattcg ccagcggatt ttttcttcat aaaaagacaa gttgtcgaat ttgacattac
      241 gcacattcat tgtcaataac tcgcatggtt ttaaatgtaa aacttaaaaa accgccaaac
      301 aaatgaagtg aaattttata cttctttgcc ttataaacaa ttgtcgcatc tttgatatta
      361 cctgcatttt ggttatctat aagttcagca atatccataa cctgcgataa atgttccact
      421 cctgtaaact cgaacctttt tcgttccctt ccggctgtaa attcatcaac ataaagctcc
      481 gccgtcaata taaaattata ctcggtgtgg tgacattcca ttctggtttc tactaaaaca
      541 ccatcatgcc agataatatt ttctacattt ttcatgctat taactattct caggtaagta
      601 accattagag tttctaaact ggttcgatga cgcaga
//
LOCUS       DAZUCE010000584          634 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZUCE010000584 DAZUCE010000000
VERSION     DAZUCE010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.587"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..599)
                     /locus_tag="ACHY03_005416"
     CDS             complement(<1..599)
                     /locus_tag="ACHY03_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3908487.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPDIEVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVT"
BASE COUNT          183 a          121 c          128 g          202 t
ORIGIN      
        1 gttacattaa aatggcagcc attaatttcc gcaacagtct tgtaatcctt gaaaaatggc
       61 ttcggttgat tattattcca tagccaatat gcagaatatg cgcctatgac aaatgcgctc
      121 aacataatta gataactttt tatatgtttg aggattttgt tattgaaatt ctttgcgcca
      181 ccaatgctct ctttgtatcc cgcattaact tctatgtcag gtggggcatt gtgtgtatgt
      241 gcgtctgttg acgagtttat tacatgattt tgtatgttaa tgtcattatg gatcttgaat
      301 cctcttctcg gcacggttgc aataagcgag tgggtagtat caccaacagc gcgaaatccg
      361 cgtctgataa tggatatgtt ctgatataac gtgttagtag gcacacgcat accttcttct
      421 tcccagactc tggtgaaaaa atcttgttgt gcaaccacat caggaaaagc ttcaagtaga
      481 agtgataaac aacgactggc tggcgttgtc agaacgacgt taagatcggc gttatgtacc
      541 gatattaatt ttcggtgctc aggccagaac tcgatattat cgttaataat ccagtacata
      601 cgtcaataaa cactaaatca atcgaaatgg aaat
//
LOCUS       DAZUCE010000585          633 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZUCE010000585 DAZUCE010000000
VERSION     DAZUCE010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.588"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>633)
                     /locus_tag="ACHY03_005417"
     CDS             complement(<1..>633)
                     /locus_tag="ACHY03_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFS3908488.1"
                     /translation="KVPLKAYYSSPEDIQKHIPFELEQQFDNLQKNPPPGTCVVACDK
                     FGEALSVFFHRMEKEKLTHMAAIVQSQTHAMAVRLRIKKTPVGETEYVVSFYDPNATN
                     TAVRYKANNCDSFGSLRSFMNIKQAQKEWVMTEICSDCVGITPYLPREQAHLLSGINN
                     ELQPPLSPLALHLLIQMGMHENIVLFFDKFKNSQEMTASKVLAAKSITGT"
BASE COUNT          179 a          113 c          124 g          217 t
ORIGIN      
        1 atgttcctgt aattgatttc gcagcaagaa cctttgatgc tgtcatttct tgggagtttt
       61 taaatttatc aaaaaaaaga acaatgtttt catgcatccc catttgtatt aatagatgta
      121 atgctaatgg tgataatggt ggttgcaact cattatttat accacttaat aaatgggctt
      181 gttctcgagg gagataagga gttattccta cacagtcgga gcaaatttct gtcattaccc
      241 attctttttg tgcctgttta atattcataa aggatcgcaa tgatccaaaa gaatcacagt
      301 tgtttgcttt atagcgtact gcagtattgg ttgcattagg atcataaaag gatacaacat
      361 attctgtttc accaacaggc gtttttttga tcctcaagcg gacggccata gcgtgagttt
      421 gagattgaac gattgccgcc atatgcgtta acttctcttt ttccattctg tgaaaaaaga
      481 ctgataatgc ctccccaaac ttatcacagg caacaacgca tgtaccgggt ggtggatttt
      541 tttgtagatt gtcaaattgc tgctcaagct cgaatggaat atgtttttgt atatcttccg
      601 gagatgaata gtaagccttt aatggaactt tcc
//
LOCUS       DAZUCE010000586          624 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZUCE010000586 DAZUCE010000000
VERSION     DAZUCE010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.589"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>624
                     /locus_tag="ACHY03_005418"
     CDS             <1..>624
                     /locus_tag="ACHY03_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908489.1"
                     /translation="KEMIFSAIKEIYNGDPDFIFDKISRNLIHTVTKSDENGISKPTD
                     LFTWHGKDKKYNTLAIVIKNTNGNDYLSLGYYDQDNYHIKRGIRINGDSLTQYCSENA
                     RNASASFESNKALMAELFATGINHQVVNELNGERLKEPNEVFKRYGRAIRYNFQVDNA
                     KYRRNNVKEIVSTLFDNEVNTDHSQNIYVHYKKLEGKIEERLQNCQT"
BASE COUNT          252 a          106 c          110 g          156 t
ORIGIN      
        1 caaggaaatg atcttcagcg caataaaaga aatatataat ggagatcctg atttcatttt
       61 cgataaaata tcacgtaacc tcatacatac ggtaacgaaa tccgatgaga acgggataag
      121 caaaccaacg gacttattta cctggcacgg taaagataaa aaatacaaca ctctcgccat
      181 tgtaattaaa aatacaaacg gaaacgatta tttatctctc ggttactacg atcaggacaa
      241 ctaccacatt aaaagaggaa ttcgtattaa tggtgatagt ctcacacaat attgtagtga
      301 aaacgccagg aatgcttcag cgtcatttga aagcaataaa gctctcatgg cagaattatt
      361 tgcaacaggt ataaatcatc aagttgtaaa cgaactcaac ggggaaagac tgaaagaacc
      421 aaacgaagtt tttaaacgtt atggtcgagc aataagatat aattttcaag tggacaatgc
      481 aaaatataga cgcaataatg taaaagaaat agtttctact ttattcgata acgaagttaa
      541 cactgaccat tctcaaaata tatatgtgca ctataagaaa ctcgaaggta aaattgaaga
      601 aaggcttcaa aattgccaga cgga
//
LOCUS       DAZUCE010000587          615 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZUCE010000587 DAZUCE010000000
VERSION     DAZUCE010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.590"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..175)
                     /locus_tag="ACHY03_005419"
     CDS             complement(<1..175)
                     /locus_tag="ACHY03_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HFS3908490.1"
                     /translation="MNKPVISRAEQIFYPGWLMVCQLRSGQPVEDGKALYRRACQLVK
                     QAREELAEAGFSQE"
     gene            complement(172..>615)
                     /gene="tssK"
                     /locus_tag="ACHY03_005420"
     CDS             complement(172..>615)
                     /gene="tssK"
                     /locus_tag="ACHY03_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3908491.1"
                     /translation="FPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGALHDARLREG
                     ADFWLSVRSSMPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTHVPAAIPLR
                     LENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
BASE COUNT          147 a          197 c          153 g          118 t
ORIGIN      
        1 tttcctgact gaatcctgct tcggccagct cttcacgcgc ctgtttcacc agctgacagg
       61 cacggcgata cagggcctta ccatcctcaa ccggttgtcc gctgcgcagc tggcagacca
      121 tcagccagcc gggataaaaa atctgttcag cccgggagat aacaggttta ttcatgtccg
      181 cagtaccgca aaaagttcca gtttcacatc ccccagcgat gccggggtgt agaaagtaca
      241 acgccccgcg tccagcatcg cccgggccgc atccgtgctt aaatccagcg cgaaatactg
      301 gttttccagc cggagcggaa tggccgccgg tacatgcgta accggacgga taatgacccc
      361 gctcagcgcc acattcacca cctcagacac atcatcgggg ctcccggctt tgcataactg
      421 cggaaacttc gtctgcagtt catgcccggg catggaagag cgcactgaaa gccagaaatc
      481 cgccccttcg cgcagacgcg catcgtgtaa tgccccctcc cacatcacac cattttgttt
      541 cagttcaatg aaaaccacac gggaaggcag gcttgcctcc agcagctggt tcagcaaaga
      601 aagcaaaggt gggaa
//
LOCUS       DAZUCE010000588          615 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZUCE010000588 DAZUCE010000000
VERSION     DAZUCE010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.591"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..175)
                     /locus_tag="ACHY03_005421"
     CDS             complement(<1..175)
                     /locus_tag="ACHY03_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HFS3908492.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQE"
     gene            complement(172..>615)
                     /gene="tssK"
                     /locus_tag="ACHY03_005422"
     CDS             complement(172..>615)
                     /gene="tssK"
                     /locus_tag="ACHY03_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3908493.1"
                     /translation="FPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGALHDARLREG
                     ADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTHVPAAIPLR
                     LENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
BASE COUNT          150 a          194 c          151 g          120 t
ORIGIN      
        1 tttcctgact gaattctgct tcagccagct cttcacgcgc ctgcgtcacc agctgacagg
       61 cacggcgata cagggcctta ccatcctcaa ccggttgtcc gcagcgcagc tggctgacca
      121 tcagccagcc gggataaaaa atctgttcag cccgggagat aacaggttta ttcatgtccg
      181 cagtaccgca aaaagttcca gtttcacatc ccccagcgat gccggggtgt agaaagtaca
      241 acgccccgcg tccagcatcg cccgggctgc atccgtactt aaatccagcg cgaaatactg
      301 gttttccagc cggagcggaa tggccgccgg tacatgcgta accggacgga taatgacccc
      361 gctcagcgcc acattcacca cctcagacac atcatcgggg cttccggctt tgcatagctg
      421 cggaaacttc gtctgcagtt catgcccggg tatggaagag cgcactgaaa gccagaaatc
      481 cgccccttcg cgcaaacgcg catcatgtaa tgccccctcc cacatcacac cattttgttt
      541 cagttcaatg aaaaccacac gggaaggcag gcttgcctcc agcagctggt tcagcaaaga
      601 aagcaagggt ggaaa
//
LOCUS       DAZUCE010000589          607 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZUCE010000589 DAZUCE010000000
VERSION     DAZUCE010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.592"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..531
                     /locus_tag="ACHY03_005423"
     CDS             <1..531
                     /locus_tag="ACHY03_005423"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFS3908494.1"
                     /translation="DEDVLLLAVGRLTDAKNYKNMLKALQIVIENKRVHLAIIGNGPD
                     RNKLIDYASRLGITQNVHWLGVRFDVYEWMSAMDLYVMSSSWEGMPLVICEAMSCGGI
                     VVATDCGGVKEIVGNSGFIVPKNDSKALSKSISDALALTEEERVSITSSARDRIERYY
                     SLNIISGKWLSLYDEF"
BASE COUNT          193 a           84 c          135 g          195 t
ORIGIN      
        1 gatgaggatg tattattact agccgttggt cgattaactg atgcgaaaaa ttataaaaat
       61 atgctaaagg cactgcaaat agttattgaa aataaacgtg tgcatctcgc cattataggt
      121 aatggtcctg atagaaataa actcatagat tatgcttctc gattaggaat aacccaaaat
      181 gtgcattggt taggagttcg ttttgatgtt tatgaatgga tgtcagcgat ggacttgtat
      241 gttatgtctt cttcatggga aggtatgcct ttagttatat gtgaggctat gtcttgtggc
      301 ggaattgttg tagcgacaga ttgcggagga gttaaagaaa tagttggcaa ttctggcttt
      361 attgtgccaa aaaatgattc caaagcatta tcaaaatcaa tatccgatgc attagctctt
      421 acagaggaag agagagtttc aatcacatct tctgcaagag ataggattga acggtactat
      481 tccttaaata ttatttcggg aaaatggtta agtttatatg acgaattctg atactagcga
      541 cctgtacaat aatcaaaaaa atattgtgtt tgttattact aagtctgaag tcggtggggc
      601 ccaaact
//
LOCUS       DAZUCE010000590          604 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZUCE010000590 DAZUCE010000000
VERSION     DAZUCE010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.593"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>604
                     /locus_tag="ACHY03_005424"
     CDS             <1..>604
                     /locus_tag="ACHY03_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908495.1"
                     /translation="LNYSRNTLDNLRGDLDQATISSSQSSSSAEQSSIKISKYNILDM
                     LDNLTMRQQAVMEEFLRVFTSMYKASAMSEAIMSNVFEQLVQYQKTETVHATMASSAG
                     VLAGSTVGAAGAAASVVANFRSHPEKMQTLTPDQKHTISGTDDKTQRGLKREVQDQQA
                     YLKQIDEEIKTTTKDTTLNEEQKEEILKYYDKEKETTEEKI"
BASE COUNT          232 a          111 c          118 g          143 t
ORIGIN      
        1 tcttaattac tcaaggaata cgctagataa tttgaggggt gaccttgacc aagctacaat
       61 ttcatccagt caatcaagca gttctgcaga acagagttct attaaaatat ctaaatataa
      121 tatccttgat atgctagata atttgaccat gcgacaacag gctgttatgg aggaattttt
      181 aagggtattc acatcaatgt ataaagcctc tgctatgtct gaggctatta tgagcaacgt
      241 ttttgaacaa ctagtccagt atcagaaaac agaaaccgta cacgccacaa tggcaagttc
      301 ggctggtgtt cttgccgggt caacagttgg cgcagctgga gctgcagcat ctgttgtagc
      361 aaatttcaga agccatcccg aaaagatgca aacccttaca cctgatcaga agcacactat
      421 cagtggaact gatgataaaa cacagcgagg attaaaaagg gaagtacaag atcaacaagc
      481 ttatctcaag caaatagatg aagaaataaa aacaacaacg aaagatacaa ctcttaatga
      541 agaacaaaaa gaagaaattc tgaaatatta cgataaagaa aaagaaacta cggaagaaaa
      601 gatt
//
LOCUS       DAZUCE010000591          602 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZUCE010000591 DAZUCE010000000
VERSION     DAZUCE010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.594"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
BASE COUNT          164 a          142 c          145 g          151 t
ORIGIN      
        1 aatcaatggg ttaagtgctc tttaacatga tggatgtgtt gtttgtgtga tactgtaaag
       61 ttggtagatt gtgactggct taaaaaatca ttaattaata ataggttatg tttagagagt
      121 tccccgcgct agcggggata aaccgttatc gcggcattga accggaggat attttatgag
      181 ttccccgcgc tagcggggat aaaccggttt tctccaccgg gtgccgttat cgttgatcga
      241 gttccccgcg ctagcgggga taaaccgagc gtaataattt ccgcaaaggc aacttcaaag
      301 agttccccgc gctagcgggg ataaaccgac cacatccacg ccgccgatag tcagagtgat
      361 gagttccccg cgccagcggg gataaaccga cgaaaaacag gccgcgagtc tcgccgatca
      421 agagttcacc gcgccagcgg ggataaaccg ccgcaaaaga tattattttt atccgtcagt
      481 acgagttccc cgcgccagtg gggataaacc accatataac ccgttatctc tttctcaagt
      541 ttttatatta gcagtacttg taataagcaa catatccacg taacacctca tgttcaaaat
      601 ag
//
LOCUS       DAZUCE010000592          600 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZUCE010000592 DAZUCE010000000
VERSION     DAZUCE010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.595"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(210..476)
                     /locus_tag="ACHY03_005425"
     CDS             complement(210..476)
                     /locus_tag="ACHY03_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFS3908496.1"
                     /translation="MVKGVVLFGDKTTHGGQVISASSNITVNGKKVALVGDMVSCPVS
                     GHGVNPILEGMPQRTCNGRSVVADGCKCQCGCQVISSAINSTIG"
BASE COUNT          217 a          148 c           84 g          151 t
ORIGIN      
        1 aagaaataac aaaaaccaga ttccaggtgg agttaatgaa aaccaccaga tacttaacat
       61 attcaacgtt ttcacatgcc cagaagcatg aataataaac aataggatac caacaataat
      121 cactaatata ccgctcagtg accaccatat cagggaagga gcagatggtg atattaaaag
      181 agatatccga tttcttttcc agcccataat taacctatcg tgctattgat agcactggaa
      241 ataacctgac aaccgcactg acacttacag ccatcagcaa caacagatcg tccattgcag
      301 gtcctctgtg gcattccttc cagtatagga ttaacgccat gtccggatac aggacagcta
      361 accatgtccc ctacaagggc aaccttctta ccattaacag taatatttga tgaagcagaa
      421 attacctgtc cgccatgtgt tgttttatca ccaaacaata caaccccctt aaccatacac
      481 cctcctatat tttcatcgaa caaatttatt acaaaaagct tccgcacatc tctatttaag
      541 acaaactttt tcttgcttaa tgtaactaaa accacaacca gcaacagaaa ataatatcaa
//
LOCUS       DAZUCE010000593          594 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZUCE010000593 DAZUCE010000000
VERSION     DAZUCE010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.596"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            2..>594
                     /locus_tag="ACHY03_005426"
     CDS             2..>594
                     /locus_tag="ACHY03_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HFS3908497.1"
                     /translation="MIYFVKTVIIIQQMIEGRLTSSVVQNDIAIYYLFRQMSLCILIF
                     LALVNKVSENTKQRNLFSKKMTLCISLFFVLGGPIVAHILSSHYESYDLHIAELTNEN
                     GQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNVSLLFMSRY
                     SVSIWYISRTIEVVSKLTVMVIFMCHIFSALRVTKDIA"
BASE COUNT          164 a           88 c          123 g          219 t
ORIGIN      
        1 tttgatttac tttgttaaaa ccgtcattat cattcagcaa atgattgagg ggcgtttgac
       61 aagcagtgtt gttcaaaatg acattgcaat ttattatttg tttcgtcaga tgagtttgtg
      121 catattaata tttctggcat tggtgaataa agttagtgaa aacacaaaac agcgcaattt
      181 attttccaaa aaaatgactt tgtgcataag tttgtttttt gttcttgggg ggccgatagt
      241 tgctcacata ctttctagcc attatgagag ctacgattta cacattgccg agttaaccaa
      301 tgaaaatggt caagtggttt ggaaagcttc atatgttacc ataatgattt tcatgtggtt
      361 aaccttgctt tcggttaatc tatactttaa tggtttacgc tatgatatct ggaatggagt
      421 gacggtgatt gctttttgtg ccgtactgta caatgtctct cttttattta tgagcaggta
      481 tagcgtgtcc atttggtata ttagtcgcac tattgaagta gtcagtaagt taaccgttat
      541 ggtaatattt atgtgccata tattcagtgc gctacgagta acaaaggaca ttgc
//
LOCUS       DAZUCE010000594          592 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZUCE010000594 DAZUCE010000000
VERSION     DAZUCE010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.597"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            2..>592
                     /locus_tag="ACHY03_005427"
     CDS             2..>592
                     /locus_tag="ACHY03_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFS3908498.1"
                     /translation="MINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSL
                     YLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPL
                     VDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL
                     LAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEI"
BASE COUNT          177 a          112 c          133 g          170 t
ORIGIN      
        1 catcattaac tatcaactgg tatcggaacg cgcggtaaaa cgtgccgata gccgctttga
       61 acttattcag aaaaacgttg gctatttctt taaagatatt gaacgttcgg ccctgacatt
      121 aaaggactca ctgtatttgt taaaaaatac agaggagatt caacgcgctg tgatcctgaa
      181 aatggaaatg atgccatttt tagactcggt gggactggta cttgatgata ataaatatta
      241 tctcttttcg cggagggcga acgataaaat cgttgtttat catcaggaac aagtaaatgg
      301 accgcttgtc gacgagtcag ggcgggttat ttttgccgat tttaacccat cgaaacgacc
      361 gtggtcggtg gcttcagatg actctaacaa cagctggaat ccggcataca attgctttga
      421 tcgtccgggt aaaaaatgta tctcttttac gctacgcatc aacggcaaag atcacgattt
      481 gttagcggtg gataaaattc atgtcgattt aaactggcga tatctgaacg agtatcttga
      541 tcaaatcagc gctaatgatg aagttctatt tttgaaacaa ggccatgaga tc
//
LOCUS       DAZUCE010000595          591 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZUCE010000595 DAZUCE010000000
VERSION     DAZUCE010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.598"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>591
                     /locus_tag="ACHY03_005428"
     CDS             <1..>591
                     /locus_tag="ACHY03_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908499.1"
                     /translation="GLLGGAAAGALAMNAETSEKLGLAKSYGVGIEKYAAWENIGKAA
                     GLNGENVGDLSEELTNKIGEVGNEKNLNPMLFQIGLTKKRMAKWDREKQFNEVMRRIS
                     EMKDDQQAASLADQLMGGEANKIMTYMRATGKSWEQTMSDAQKSNLLTKEGAEGAARA
                     HVSVTNLWGAITSGLADTLGKIGGELAPTFDAMRDEL"
BASE COUNT          170 a          114 c          195 g          112 t
ORIGIN      
        1 gggctgctcg gcggcgcggc tgctggcgcg ctggcaatga atgcagagac gtcagaaaag
       61 ctcggtctgg cgaagtccta cggtgttggc atcgaaaaat acgctgcgtg ggaaaatatt
      121 ggtaaagctg cagggctgaa tggcgaaaac gtaggagatc tgtcggagga gttgactaat
      181 aaaatcgggg aggttggaaa cgaaaaaaac ctgaatccca tgttgtttca aattggcctg
      241 acaaaaaaac ggatggctaa atgggatcga gaaaaacagt tcaatgaagt tatgcggcgt
      301 atctctgaaa tgaaggatga tcaacaggcg gcgagtctgg ctgatcagct catgggggga
      361 gaggctaata aaataatgac ctatatgcgg gcgactggta aaagctggga gcagactatg
      421 tcggacgctc aaaaatcgaa cctgctaacg aaagaagggg ctgagggggc agcgcgcgcg
      481 cacgtatccg tcacgaatct atggggggca attacatcgg ggttggcaga tacgctcggt
      541 aaaatcgggg gggaactcgc gccgacgttt gacgctatgc gagacgagct g
//
LOCUS       DAZUCE010000596          589 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZUCE010000596 DAZUCE010000000
VERSION     DAZUCE010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.599"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>589)
                     /gene="trkG"
                     /locus_tag="ACHY03_005429"
     CDS             complement(<1..>589)
                     /gene="trkG"
                     /locus_tag="ACHY03_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Trk system potassium transporter TrkG"
                     /protein_id="HFS3908500.1"
                     /translation="GAWYTTKKSGIQLRTRDGFIIIVMFWILFSVISAFPLWIDSELN
                     LTFIDALFEGVSGITTTGATVIDDVSSLPRAYLYYRSQLNFIGGLGVIVLAVAVLPLL
                     GIGGAKLYQSEMPGPFKDDKLTPRLADTSRTLWITYSLLGIACIVCYRLAGMPLFDAI
                     CHGISTVSLGGFSTHSESIGYFNNYLVELVAGSFSL"
BASE COUNT          219 a          138 c           97 g          135 t
ORIGIN      
        1 agggaaaaag aaccagccac cagctcaacc aaatagttat taaaatatcc gatactctcg
       61 ctatgagttg agaaaccacc aagcgaaact gtggaaatcc cgtgacaaat agcatcaaac
      121 aaaggcattc ctgcaagtct ataacagaca atacaagcaa tacctaataa agaataagtt
      181 atccacagtg tccgtgacgt atcggccagg cggggagtga gtttgtcatc cttaaatggc
      241 cccggcattt ctgactgata aagctttgca ccaccaatac ccaataatgg caatacagca
      301 accgccagaa caataactcc taaaccacct ataaaattta actgtgaccg atagtacaaa
      361 tatgcccgag gtaatgaact aacatcatca attacagttg ctcctgttgt tgttattcca
      421 gaaacccctt caaacagagc atcaatgaac gttaaattaa gttctgagtc aatccataaa
      481 gggaatgcac taataacaga aaacaaaatc caaaacatta caattataat aaacccatca
      541 cgggtacgta attgaatgcc agatttctta gttgtatacc acgctccgc
//
LOCUS       DAZUCE010000597          581 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZUCE010000597 DAZUCE010000000
VERSION     DAZUCE010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.600"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..290)
                     /locus_tag="ACHY03_005430"
     CDS             complement(<1..290)
                     /locus_tag="ACHY03_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFS3908501.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCP"
BASE COUNT          177 a          134 c          138 g          132 t
ORIGIN      
        1 gggcacacct tcatgctgta cagcatctgg tttttgtcgt tctcccccag ttcaaaccag
       61 gtcaccacac ctttcacccg gcgctgtacg tcatcgccct gccataccgt caggtaggcc
      121 attttgtcca gcacctgcgc aaattcaagg gaaagaaact gctgactgac cagagaaagg
      181 tcgagggaaa aaagtgaaga gagtgactgg gtgagatgaa aggaaaccac cgcaaaagca
      241 tccggtggca ggccgtccac ttccagcgtg aaacgtaatc cggttgacat agcaacctcc
      301 gtgaaaatga ataataatcc gcacatattg catgtacaac atgtcgggcg gatgagataa
      361 gcgagtcgac atctggcgtt accggggaat atcagaaaaa tgtcatggtt atgtgatgat
      421 ttagtctggg attttacttc agagaaacgg gtgtctgttt agtcaacaaa ataatcacaa
      481 acgcaacgga tgggttctcg acgttattca cgacaggctc gtacattaca gcgagtaata
      541 aaaaacctga tatgcaaagt caaaccaata ttcataatgt c
//
LOCUS       DAZUCE010000598          579 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZUCE010000598 DAZUCE010000000
VERSION     DAZUCE010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.601"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>579
                     /locus_tag="ACHY03_005431"
     rRNA            <1..>579
                     /locus_tag="ACHY03_005431"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          163 a          127 c          177 g          112 t
ORIGIN      
        1 aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa
       61 ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca
      121 tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa
      181 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
      241 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      301 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      361 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      421 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      481 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      541 cggcctgcgc ggaagatgta acggggctaa accatgcac
//
LOCUS       DAZUCE010000599          569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZUCE010000599 DAZUCE010000000
VERSION     DAZUCE010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.602"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>569
                     /locus_tag="ACHY03_005432"
     CDS             <1..>569
                     /locus_tag="ACHY03_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908502.1"
                     /translation="IQALKKLTCAISQTLEEQPVLNSKSWLTSLQNDYSLPDSLTERI
                     WLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKENLSFTPDELKTLFR
                     NRLNLSPEANDDFLDRCLNGGDWYPFSEGRRFVSFHHVDELRICASCGLTEVHHAPEN
                     HKPDPEWYCSSLCRETETLCQEIYERPYN"
BASE COUNT          156 a          142 c          139 g          132 t
ORIGIN      
        1 atccaggcgc tgaaaaaact gacctgtgcc atcagccaga cactggaaga acaaccggtg
       61 ctcaacagta aatcgtggct gaccagtttg caaaacgatt acagccttcc tgactcactg
      121 acggagcgca tctggctgac gctcatttct caacggattt cccggggaga gctgagggaa
      181 tttgaactgg cagacggaaa ctggttactg aacaatgcct ggtatgaaag aaacatggca
      241 gggtttaacg aacagttgaa agagaacctg tcattcacac ctgatgaact gaaaacgctc
      301 ttccggaacc gcctgaattt atcaccggaa gcgaatgacg attttctcga tcgttgcctg
      361 aacggcggtg actggtatcc cttttcagaa ggtcgccgtt ttgtatcatt ccatcacgtg
      421 gatgagcttc gtatctgtgc ctcctgcggg ctgacagaag tacatcatgc cccggaaaat
      481 cataagccgg atccggaatg gtactgctcc tctctttgtc gcgaaacaga aacactgtgt
      541 caggaaattt atgaacgccc ttacaacag
//
LOCUS       DAZUCE010000600          569 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZUCE010000600 DAZUCE010000000
VERSION     DAZUCE010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.603"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>569
                     /locus_tag="ACHY03_005433"
     CDS             <1..>569
                     /locus_tag="ACHY03_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908503.1"
                     /translation="IQALKKLTCAISQTLEEQPVLNSKSWLTSLQNDYSLPDSLTERI
                     WLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKENLSFTPDELKTLFR
                     NRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRVCASCGLTEVHHAPEN
                     HKPDPEWYCSSLCRETETLCQEIYERPYN"
BASE COUNT          155 a          142 c          140 g          132 t
ORIGIN      
        1 atccaggcgc tgaaaaaact gacctgtgcc atcagccaga cactggaaga acaaccggtg
       61 ctcaacagta aatcgtggct gaccagtttg caaaacgatt acagccttcc tgactcactg
      121 acggagcgca tctggctgac gcttatttct caacggattt cccggggaga gctgagggaa
      181 tttgaactgg cagacggaaa ctggttactg aacaatgcct ggtatgaaag aaacatggca
      241 gggtttaacg aacagttgaa agagaacctg tcattcacac ctgatgaact gaaaacactc
      301 ttccggaacc gcctgaattt atcaccggaa gcgaatgacg attttctcga tcgttgcctg
      361 gacggcggtg actggtatcc cttttcagaa ggtcgccgtt ttgtatcatt ccatcacgtg
      421 gatgagcttc gtgtctgtgc ctcctgcggg ctgacagaag tacatcatgc cccggaaaat
      481 cataagccgg atccggaatg gtactgctcc tctctttgtc gcgaaacaga aacactgtgt
      541 caggaaattt atgaacgccc ctacaacag
//
LOCUS       DAZUCE010000601          567 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZUCE010000601 DAZUCE010000000
VERSION     DAZUCE010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.604"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>567
                     /locus_tag="ACHY03_005434"
     CDS             <1..>567
                     /locus_tag="ACHY03_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3908504.1"
                     /translation="MKDEQTELLHLEKDIGRRVVGQDVALGSIAQRLRAAKTGLTSGN
                     GPQGVFLLVGPSGVGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGY
                     VGYGQGGILTEAVRKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRN
                     TVILMTSNLGSDLLMQQLSEKPETTESE"
BASE COUNT          132 a          133 c          188 g          114 t
ORIGIN      
        1 tgatgaagga cgaacagacg gaactgctgc atctggaaaa ggatatcggc agacgggtgg
       61 tcggacagga cgtggcactg ggatccattg cgcagcgtct gcgtgcggcg aaaacgggtc
      121 tgacatccgg taacggcccc cagggcgtgt tcctgctggt cggtcccagt ggtgtgggaa
      181 aaaccgaaac ggcactggcg ctggcagatg tgatgtacgg cggcgaaaaa tcactgatta
      241 ccatcaatct gtcggaatac caggaacccc atacggtttc ccagcttaag ggttcaccgc
      301 ccgggtacgt gggatacggt caggggggta ttctgacgga agctgtacgt aagcgtccgt
      361 acagtgtggt gctgctggat gaagtggaaa aggcccaccg ggatgtgctg aatctgttct
      421 accaggtgtt tgaccggggc tttatgcgcg acggtgaggg gcgtgaaatt gacttccgta
      481 atacggtcat tctgatgacc tccaatctgg gcagtgacct tctgatgcag caactgagcg
      541 aaaagccgga gacaacggaa tcggagc
//
LOCUS       DAZUCE010000602          564 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZUCE010000602 DAZUCE010000000
VERSION     DAZUCE010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.605"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(114..293)
                     /locus_tag="ACHY03_005435"
     CDS             complement(114..293)
                     /locus_tag="ACHY03_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908505.1"
                     /translation="MQHNCLITPDTPLPDPDVVRRFVLRTVVQWLCLLMLLFMRHSDR
                     GDIDKHHLRANSYTE"
     gene            complement(299..>564)
                     /locus_tag="ACHY03_005436"
     CDS             complement(299..>564)
                     /locus_tag="ACHY03_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFS3908506.1"
                     /translation="EHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWH
                     CVMCERLFHGKKFCEACGTGIYCRAVCTKSVYRYQPDLLRNPE"
BASE COUNT          161 a          160 c          111 g          132 t
ORIGIN      
        1 agaattccgt ataaagattc agctgacaaa actttatcag tccataagaa tcaacgaaag
       61 caccgatggt tcacctggtc atcaccaatt tttgccataa ccggtagcaa tgatcattct
      121 gtgtaactat ttgcccgcag atgatgttta tcgatgtctc ccctgtctga atgtcgcata
      181 aagaggagca tcagcagaca cagccattga accaccgtcc gcagaacaaa acgccggacc
      241 acatccggat ccggcagggg cgtgtctggc gtgattaaac aattatgttg catcctcctc
      301 actccgggtt ccggagcaaa tctggctggt acctgtaaac agactttgta cataccgctc
      361 tgcaataaat ccccgtacca caggcctcac agaacttctt cccatgaaaa agcctctcac
      421 acatcacaca atgccactcc agcacgaccg gtaatggcga taaaaacatc gccatatcct
      481 caatgtaagg gtgggacttt tccggattca gcaccacaca gtccgccttc tgttgctcac
      541 tcagggcatg taaatcatgc tcaa
//
LOCUS       DAZUCE010000603          558 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZUCE010000603 DAZUCE010000000
VERSION     DAZUCE010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.606"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            74..>558
                     /gene="ehxC"
                     /locus_tag="ACHY03_005437"
                     /gene_synonym="hlyC"
     CDS             74..>558
                     /gene="ehxC"
                     /locus_tag="ACHY03_005437"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFS3908507.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWLSGERKWFIDWIAPFGHNMEL
                     YKHMRKKYPYELFRAIRLDEYSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     Q"
BASE COUNT          186 a           76 c          115 g          181 t
ORIGIN      
        1 taaagaatat aatttttatg cttttatatt gatttcttta catttactga gttttcttat
       61 ttatttaaac attatgaaat ctaatgcttt tgacgtcatg gggaaggttg catggctatg
      121 ggcctgttct cctctgcata aaaaatggcc attgtctgtt tttgctatta atgtcatacc
      181 tgctattcag acaaatcaat ttgctctctt aataaaggat gagcttcctg tagctttttg
      241 tagctgggcc agcttagatc tggagtgtga ggtaaaatat ataaatgatg taacatcttt
      301 gtatgcaaaa gactggttgt cgggagaaag aaagtggttt attgactgga tagctccatt
      361 tggacacaac atggagttat ataaacacat gaggaaaaag tatccttatg agttattcag
      421 agcaataagg ttagatgagt acagtaaaac agggaaaata gctgaatttc atggaggggg
      481 gatagataaa aaactggcat caaaaatatt caggcaatac catcatgaac ttatgtctga
      541 agtaaaaaac agacaaga
//
LOCUS       DAZUCE010000604          555 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZUCE010000604 DAZUCE010000000
VERSION     DAZUCE010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.607"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
BASE COUNT          168 a          110 c          119 g          158 t
ORIGIN      
        1 tccagcaggg gccaccaaat catcattcat gtatcaggaa gagttaaggg tacaaggggt
       61 actcgatggc ggtctatatt tcatgactaa cgccatttaa aggaagaagt gcggtgggtg
      121 gtttcgaatg agggctggtg ttttggtacg tggcacgcca ctgcaatgta tttatccatc
      181 agtactcatt caaaagtacc tggaaaaaat gcccgctatg ccaagcgggc aaattgctta
      241 catgtggaat taagtaatta acgataaagc cagagtatta tcatttatta tacccattca
      301 tttaatcagg ttaattactc aaatcactga actattctct gttcgctcac tgatgatgag
      361 tgagtaccac aaccaatgta tgtagaacaa tgccgtagag taaggatgaa atgtctctgc
      421 cacgccttat acatctacta ctgactagaa cacccgccgc aggtagatga gttttgtgag
      481 cgttacagga gtctggcttt gctcattaat caacgctatt tagatataaa tttctaacac
      541 tcgaaaaacg atttt
//
LOCUS       DAZUCE010000605          553 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZUCE010000605 DAZUCE010000000
VERSION     DAZUCE010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.608"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>553
                     /locus_tag="ACHY03_005438"
     CDS             <1..>553
                     /locus_tag="ACHY03_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFS3908508.1"
                     /translation="YYQGIYLHADRIPLSAMNGNIGQMGAATIAAYVITFTTLYSSIV
                     FIKMQSKYKWFLFYANFALSFAAVMMTGTRAAIFTFPLMIMVILFLQHRDQKVFLFKG
                     LSGVFILLLACGLIFNKEIERRINSLKADVISYATKNNSQSSVGARFAMVNAGIKGSP
                     DGFNWQSLEQRAEKIKALSADNN"
BASE COUNT          167 a           85 c          112 g          189 t
ORIGIN      
        1 catattatca agggatatat ttgcatgcag accgcattcc cctctccgca atgaatggga
       61 atattggtca gatgggggca gcgactattg cggcttatgt gattacattc actactttat
      121 attcttcaat tgtattcatc aaaatgcaat cgaaatataa atggtttcta ttttatgcta
      181 actttgcttt aagctttgca gcagtgatga tgacaggaac gcgtgcggca atttttacat
      241 tcccacttat gattatggtc atattatttc ttcagcatcg tgatcaaaaa gtgtttttat
      301 ttaaaggatt gagtggtgtt tttatattgc tacttgcttg tggtttgata tttaataagg
      361 aaattgagag gcgaattaat tcattaaaag ctgatgttat ttcatatgct acaaaaaata
      421 acagtcaatc ctcggtcgga gcacgttttg caatggttaa cgcaggaatt aaaggaagtc
      481 ctgatggttt taattggcaa tctcttgagc agcgtgctga aaagataaaa gcattaagtg
      541 cagacaataa tat
//
LOCUS       DAZUCE010000606          551 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZUCE010000606 DAZUCE010000000
VERSION     DAZUCE010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.609"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>551)
                     /locus_tag="ACHY03_005439"
     CDS             complement(<1..>551)
                     /locus_tag="ACHY03_005439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HFS3908509.1"
                     /translation="CCVYLFKYYRPDFFFEKAIRYNELYFSSTEQLNDPNDLKTSYFF
                     EDRPDLWQSLLESEVILGVKNLKDFLDLGDGRLAKQLCRIFLGSPIDGSIEGLQSVFT
                     KYDSEVKAALNQHIKQVDLIDFGVLEDSDKSISRLESICSSGIRERVLKGLRVGVFSV
                     SFSSNALEPMMWAHYAAGFKGCV"
BASE COUNT          180 a          123 c           92 g          156 t
ORIGIN      
        1 taacacaacc cttgaaacca gctgcgtaat gtgcccacat cattggttct aaagcattgc
       61 tcgaaaatga aacgctaaag acacctactc gcaatccttt gagaaccctc tctctaatgc
      121 cgctggagca aatagattcc agtcgagata ttgacttatc tgaatcctcg agcacaccaa
      181 aatcaattaa atcgacctgt ttaatgtgtt gatttaaagc agcctttact tcagagtcgt
      241 attttgtgaa aacactttga agcccttcta tggagccatc aataggagag cctaaaaata
      301 tgcgacacag ttgctttgca agcctaccat cgcccaaatc cagaaagtct tttaagtttt
      361 tgacacctaa aatcacctcg ctttctagaa gactttgcca gagatcaggt ctatcctcaa
      421 agaaataaga agttttcaag tcattagggt catttagttg ctctgtagac gaaaaatata
      481 attcgttgta tctgatggct ttttcaaaga aaaaatccgg cctgtaatat ttaaatagat
      541 acacacaaca c
//
LOCUS       DAZUCE010000607          549 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZUCE010000607 DAZUCE010000000
VERSION     DAZUCE010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.610"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..274
                     /locus_tag="ACHY03_005440"
     CDS             <1..274
                     /locus_tag="ACHY03_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFS3908510.1"
                     /translation="SSVMLQTVVKKALTKYDFKVEMEHTAAGEVGGFTDWADIFAISK
                     KLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHYPHLINQ"
     gene            286..>549
                     /locus_tag="ACHY03_005441"
     CDS             286..>549
                     /locus_tag="ACHY03_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFS3908511.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIY"
BASE COUNT          150 a          114 c          140 g          145 t
ORIGIN      
        1 cagtagcgtt atgttacaaa cggtcgttaa aaaagcgctg accaaatatg atttcaaagt
       61 tgaaatggaa cataccgccg caggtgaagt cggcggattt accgactggg cggatatttt
      121 tgctatttcc aaaaaattac tcgatgtggt tagtcttgac ccgaaacaag ggcaatatct
      181 gatcccgata gagaatatta tggacggtga aactattggt cagcaaattt atgaagtggt
      241 tcagcaacat tatccgcatt taattaatca ataaggtagg gcgtaatgag agcgattatt
      301 ctgctgttcg atagtctgaa taaacgttat ctgccacctt atggcgatgc gttaaccaaa
      361 gcgcctaatt tccaacgtct ggcggctcat gccgccacct ttgaaaacag ttacgtcggc
      421 agtatgccgt gtatgcctgc ccggcgggag ttgcataccg ggcgatgtaa cttcctgcat
      481 cgggagtggg ggccgttaga accgtttgac gattccatgc cggagctgct taaaaaggcg
      541 gggatctat
//
LOCUS       DAZUCE010000608          548 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZUCE010000608 DAZUCE010000000
VERSION     DAZUCE010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.611"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            1..548
                     /locus_tag="ACHY03_005442"
                     /pseudo
     CDS             1..548
                     /locus_tag="ACHY03_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          144 a          132 c          119 g          153 t
ORIGIN      
        1 caaccctggg tcactgcatt atcgccaacg ccccgacacg taattcctga gcaattagaa
       61 aaaacagttc gttatcttac acaaactgtg catccacgta atgccgacaa tattgataat
      121 ctgaataggt ccgtagagta tataaaagag gtctttgtca gtagcggtgc cagagttacc
      181 gcgcagggcg tccccgattg gctattacga ctctgcgcct ggtagccagg attaccttta
      241 cccgcaatgt cctggcttta tcccgatcgg ggggacttta ttgcctggtc ggcagaatac
      301 aggatatcaa cgccgttcgt caggtaaaaa cggcattgtt atcatcccgg gatttatctg
      361 tttattctat gaatgcccca tggtttattc acggtattga tttctctgac caccttaatt
      421 attggcaaca cgatattccc gccgtaatga ttactgacac cgctttttat cgtaataaac
      481 aataccactt gcccggtgat accgcagaca gattgaatta tcagaaaatg gctcagatgg
      541 tggatggt
//
LOCUS       DAZUCE010000609          547 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZUCE010000609 DAZUCE010000000
VERSION     DAZUCE010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.612"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>547)
                     /gene="trkG"
                     /locus_tag="ACHY03_005443"
     CDS             complement(<1..>547)
                     /gene="trkG"
                     /locus_tag="ACHY03_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkG"
                     /protein_id="HFS3908512.1"
                     /translation="RKTIKPLIRDIELRFFLLIALGVIIVTSFQVWHIGMYDLHGSFI
                     HSFFLASSMLTDNGLATQDYASWPTHTIVFLLLSSFFGGCIGSTCGGIKSLRFLILFK
                     QSKHEINQLSHPRALLSVNVGGKIVTDRVMRSVWSFFFLYTLFTVFFILVLNGMGYDF
                     LTSFATVAACINNMGLGFGATA"
BASE COUNT          203 a          122 c           86 g          136 t
ORIGIN      
        1 atgcagtagc cccaaaacct aatcccatat tattaataca tgcagccact gttgcaaatg
       61 atgtaagaaa atcatatccc ataccattta acaccagtat aaaaaacacc gtgaagagag
      121 tataaagaaa aaagaaactc catacagacc tcattacacg atctgtaact atcttccctc
      181 ctacatttac actcaacaac gctctgggat gagaaagctg atttatctcg tgtttgcttt
      241 gtttgaaaag tataagaaat cgaagtgact taattccacc acaagttgaa cctatacatc
      301 ccccaaagaa acttgacaac agcaaaaaca ctatcgtgtg cgtgggccaa cttgcataat
      361 cctgcgtagc taaaccatta tcagtgagca tggagctggc aagaaaaaac gaatgaataa
      421 aacttccatg caagtcatac atacctatat gccagacctg gaaagaggta acaatgatca
      481 cccctaaggc tattaacaga aagaaacgaa gttcaatatc tctgattaaa ggttttatcg
      541 ttttcct
//
LOCUS       DAZUCE010000610          544 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZUCE010000610 DAZUCE010000000
VERSION     DAZUCE010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.613"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            49..432
                     /locus_tag="ACHY03_005444"
     CDS             49..432
                     /locus_tag="ACHY03_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFS3908513.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGHECPYVRPERREIQLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGERYCTHCQTGRFSEEGVAE"
     gene            429..>544
                     /locus_tag="ACHY03_005445"
     CDS             429..>544
                     /locus_tag="ACHY03_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908514.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGR"
BASE COUNT          125 a          151 c          155 g          113 t
ORIGIN      
        1 gggacggctg ttgcctgcgt gtcgtctggc ggctgaggaa catatactat gtacgcaaaa
       61 tcctttctcg ctcttgatgg caacggacgt ctgacggggg cccgtaccgc acagactgcg
      121 ccttacgacc gctacacctg ccacctttgc ggcagtgcac tcagatacca cccgcaatac
      181 gacactgaac gtccctggtt tgaacacact gacgacgggc tgacggagca cggtcatgag
      241 tgcccttatg tcagaccgga gcgcagggag atacaactga ttaaacgtct gcagcaattc
      301 gtaccggatg ccttacccgt agtacgtaaa gccagctggt actgcagaca atgtcaccac
      361 gattattatg gggagcggta ctgcacacac tgccagaccg ggcgcttcag tgaggagggg
      421 gtagcagaat gaacggtata aaaacggggc tggggatcac gccgggtgag cacattatca
      481 gtgccgactc tgcgctcagc agaaatatcc ggcattgttt ctgcctttcc tgccatggcc
      541 ggtt
//
LOCUS       DAZUCE010000611          540 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZUCE010000611 DAZUCE010000000
VERSION     DAZUCE010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.614"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(342..>540)
                     /locus_tag="ACHY03_005446"
     CDS             complement(342..>540)
                     /locus_tag="ACHY03_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3908515.1"
                     /translation="IRQGLGIALLPELTLKTIADELCSVPLEPTFYRQISLLAKEKPV
                     EGSPLFLLQMCTEQLVVSGKI"
BASE COUNT          160 a          115 c          109 g          156 t
ORIGIN      
        1 ttaataaata cggtaataag taacaaacag atattgataa cttccctgac aagttattag
       61 gtatcccact tgaagattga gaatggctcc agagtaaact gtgtactgtt cgccggagtg
      121 tcattactct caacaatcgg atttgacagt gagtcgggcg cttcataccg gtttctacct
      181 ggagtcattg tcacttctcc caactggata aatatatcgc caattcacaa agtgggccaa
      241 aatcaggtga aaacgaatta ttacgcctgg taaaggctat agtacaacta agtgtggcga
      301 atcaggcagt cttgcatttg tcatcaatct catgtcttac atcaaatttt tccactcacc
      361 actagttgtt ccgtacacat ttgtagtaaa aacagtggac tgccttctac cggcttttct
      421 ttagccagca acgaaatctg tcgatagaag gttggttcga gaggaacgga acacaattca
      481 tctgcaatgg ttttcagcgt taactctggt aataaggcga tgcctaatcc ctgtcgaata
//
LOCUS       DAZUCE010000612          539 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZUCE010000612 DAZUCE010000000
VERSION     DAZUCE010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.615"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..334
                     /locus_tag="ACHY03_005447"
     CDS             <1..334
                     /locus_tag="ACHY03_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFS3908516.1"
                     /translation="TEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEKMESNSDSAPY
                     TLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDANINHYYRNLEIKHSQPKN
                     RASSKRVS"
BASE COUNT          182 a          106 c          100 g          151 t
ORIGIN      
        1 aactgaacca gagataatat ttttacatgc cgatcttcag caatacttaa gccaaagttg
       61 cggtgcattt gtgtgcatgg cagcccagga agtgattgaa aaaatggaaa gcaattctga
      121 cagcgccccc tatacgttat taaaaaacta tgctgacaga tttaaaaaat attcagcaga
      181 agagcagtac gaaattgatt ttcaacatcg actggtaaac agaaattgtt atttagataa
      241 atatggcgat gcaaatatca atcattatta tagaaactta gaaataaaac actcacaacc
      301 caaaaataga gcatcaagca aaagagtaag ttaacatcat tgttaattaa ttcgaccagt
      361 cagcaaatat gctgactggt cgaacatagt ttgctaaaaa gtcatcactg actgatgtca
      421 gtcacaccac ccgccagttc aatcttcggg tatttccagc gtatctcggc acgcgattgc
      481 tggtctgttg atgtagtgga ttgttgttgc tgacttttct taccctcaga tttctgccc
//
LOCUS       DAZUCE010000613          539 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZUCE010000613 DAZUCE010000000
VERSION     DAZUCE010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.616"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>539
                     /gene="eilA"
                     /locus_tag="ACHY03_005448"
     CDS             <1..>539
                     /gene="eilA"
                     /locus_tag="ACHY03_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HFS3908517.1"
                     /translation="ILLLNADGEIVSKEEIIDKVWSASVASDESLTRCIYALRKLLHE
                     NKQCKYIETVYGRGYRFTVPIVIVTDNEPVKSTTTLAVFPFRTEGSINVLKLHYELVQ
                     GLSKYAFCGLDILPASVTNEASDFSTIHQFINKTGPEYYIMGQVVHYGKNWRLFIELI
                     HARTHKLIDHQSIDFNPDN"
BASE COUNT          156 a          102 c          106 g          175 t
ORIGIN      
        1 aattctatta cttaatgctg acggtgaaat tgttagcaaa gaagagatta tcgataaggt
       61 ctggagcgcg agtgttgcta gtgatgaatc tttaacacgg tgtatttatg cattacgtaa
      121 acttttgcat gagaataaac agtgtaagta tatcgaaacg gtctatggtc gtggataccg
      181 ttttactgtc cccattgtga ttgtaaccga taatgaaccg gtaaagtcga ccaccactct
      241 tgccgttttt ccctttcgta ctgaaggttc gatcaatgtt ctaaaactcc attacgaact
      301 ggtccagggc ctttcaaaat atgctttttg tggtttagat attttacccg cttcggtaac
      361 caatgaggcc agtgatttct ccactatcca tcagtttatc aataaaacag gtccagaata
      421 ttatattatg ggtcaggtcg ttcactatgg taaaaactgg cgtttattta ttgagttaat
      481 ccatgccaga acgcataaat tgattgatca tcaaagtatt gatttcaacc ccgacaatc
//
LOCUS       DAZUCE010000614          539 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZUCE010000614 DAZUCE010000000
VERSION     DAZUCE010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.617"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            44..>539
                     /locus_tag="ACHY03_005449"
     CDS             44..>539
                     /locus_tag="ACHY03_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3908518.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADA
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATS"
BASE COUNT          162 a          101 c          129 g          147 t
ORIGIN      
        1 ttaaaggcat tatcatattt acgaagagat caggatgata acgatgaaaa aaagtgtatt
       61 gacggcgttt ataactgtgg tatgtgcaac gtccagtgtt atggcagctg atgataatgc
      121 tatcacggat ggttcagtaa catttaatgg taaagttatt gctccagctt gtaccctggt
      181 agctgcgacg aaagattccg tggtgacttt gccagatgtt agtgccacga agttgcaaac
      241 caatggtcag gtttctggcg tgcaaactga tgtgccaatt gaattaaaag attgtgatac
      301 taccgtaaca aaaaatgcaa cgttcacctt taatggcact gcggatgcta ctcagattac
      361 agcgtttgct aaccaggcct catctgatgc tgctacaaac gtggccctgc aaatgtatat
      421 gaatgatggt acaacggcca tcaagccaga cacagaaacc gggaacattt tgttgcaaga
      481 tggagatcag acgttgactt ttaaagttga ttatatcgct acggggaaag cgacttctg
//
LOCUS       DAZUCE010000615          531 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZUCE010000615 DAZUCE010000000
VERSION     DAZUCE010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.618"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>531
                     /gene="ehxB"
                     /locus_tag="ACHY03_005450"
                     /gene_synonym="hlyB"
     CDS             <1..>531
                     /gene="ehxB"
                     /locus_tag="ACHY03_005450"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFS3908519.1"
                     /translation="VIWSLFISPLLRRRLDDKFLRNAENQAFLVETVTAINTIKSMAV
                     SPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVISLWMGAHLVISGEIS
                     IGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNTPVEKKSGRNILPEI
                     QGDIEFKNVRFRYSSDG"
BASE COUNT          153 a           90 c          132 g          156 t
ORIGIN      
        1 tgttatatgg tcattgttta tatcaccctt attacgtcga cgtcttgatg ataagtttct
       61 caggaatgca gaaaatcaag cttttcttgt cgaaacggta acagcaataa atacaattaa
      121 atccatggca gtatcgccac agatgattgc tacatgggat aaacaactgg ccgggtatgt
      181 tgcttccagt ttcagggtaa atctggttgc aatgacaggg cagcagggga tacagctgat
      241 acagaaaagt gtaatggtaa ttagtctatg gatgggagca catcttgtca tatcgggaga
      301 gataagcatc gggcagttaa tcgcgtttaa tatgcttgca ggtcaggtca ttgctcctgt
      361 tatcagactg gctcatcttt ggcaggattt tcagcaagtt ggtatttctg ttgagcgcct
      421 tggggatgta ttaaatacac cggtagagaa aaagtcaggc agaaatatac tgccggaaat
      481 tcagggggat atcgaattta aaaatgtcag atttcggtat tcttctgacg g
//
LOCUS       DAZUCE010000616          526 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZUCE010000616 DAZUCE010000000
VERSION     DAZUCE010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.619"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            23..>526
                     /locus_tag="ACHY03_005451"
     CDS             23..>526
                     /locus_tag="ACHY03_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908520.1"
                     /translation="MCDFIKSCYAAPRINKMADFFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTSPTKENEPVFFSHGSHIHSYFICSPFFLVHEEIKNN
                     NPELFPHG"
BASE COUNT          180 a           98 c           85 g          163 t
ORIGIN      
        1 ataagttata caggatagtt aaatgtgtga ttttataaaa tcatgttatg cagcaccaag
       61 aattaacaaa atggctgatt ttttctcatc aacagcatct ggagtaaaac ataaaccttt
      121 gaagcatgta aaaattgata acaggctttt cccatctcct gagtatataa ttttttctga
      181 ctatcttacg aaaaacagtg gtccatttgt taaccactac ttttgcagta ttccatacag
      241 caaagaagaa gaatgcaggc tgggtgtgac tattatgaaa tatgcaagcc atcgaaataa
      301 gccattgagc ttatggtgtc ttggaatggc agaaggagcc atggccagaa caatttctca
      361 actatcaaaa ggacaggttt tatcattaac aaccagccca acaaaagaaa atgaacctgt
      421 attctttagt catggttctc atattcactc ttactttatt tgctccccat tctttcttgt
      481 tcatgaagaa ataaaaaata acaaccctga actatttcct catggt
//
LOCUS       DAZUCE010000617          524 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZUCE010000617 DAZUCE010000000
VERSION     DAZUCE010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.620"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>524
                     /locus_tag="ACHY03_005452"
     CDS             <1..>524
                     /locus_tag="ACHY03_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3908521.1"
                     /translation="PRHRGLSHWHRLCLQTGAIVGVFLLLAMLASFMNNQRLVRSVGE
                     HLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQQRGVPQRYGLGLYQGMWLIPPVER
                     ATGEWIAPLPPRPVIQKVTQTPKMVRLNSLALFDAGKWTLKPGATKWLVNALVDIKAK
                     AGWLIVVSGHTDNTG"
BASE COUNT          116 a          130 c          161 g          117 t
ORIGIN      
        1 ccccgtcatc gtggactcag tcactggcac agactttgtc tccagacggg ggcgattgtc
       61 ggcgtttttc ttctgctggc tatgctggcg tcttttatga ataaccagcg actggttcgc
      121 agcgtggggg aacacctgac ggcctggcac cggatgcgcg gatattcatc tgaatcgaaa
      181 aaaactgcac tgcaacgtct tcaggctgac agccagttac tgaacgactg gcagcaacgt
      241 ggtgtgccgc agcgttacgg actgggattg tatcagggaa tgtggctgat accgccggtt
      301 gagcgagcga ctggtgagtg gattgcacca ctgcctcccc gtccggtaat acagaaagtt
      361 actcagacgc cgaaaatggt tcgccttaac agcctggcgc tgtttgatgc cggtaaatgg
      421 acgctgaaac cgggcgccac aaaatggctg gtgaatgccc tggtggatat taaggcaaaa
      481 gcgggctggc tgattgtggt cagtggtcac acggacaata cggg
//
LOCUS       DAZUCE010000618          524 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZUCE010000618 DAZUCE010000000
VERSION     DAZUCE010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.621"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>524)
                     /locus_tag="ACHY03_005453"
     CDS             complement(<1..>524)
                     /locus_tag="ACHY03_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3908522.1"
                     /translation="PRHRGLSHWHRLCRQTGLIVGVFLLLAMLASFMNNQRLVRSVGE
                     HLTAWHRMRGYSSELKTTALQRLQADSQLLNDWQQHGVPQRYGLGLYQGMWLIPPVER
                     ATGEWVAPLPPRPVIQKVTQTPKTVRLNSLALFDAGKWTLKPGATKWLVNALVDIKAK
                     AGWLIVVSGHTDNTG"
BASE COUNT          119 a          162 c          128 g          115 t
ORIGIN      
        1 cccgtattgt ccgtgtgacc actgaccaca atcagccagc ccgcttttgc cttaatatcc
       61 accagggcat tcaccagcca ttttgtggcg cccggtttca gcgtccattt accggcatca
      121 aacagcgcca ggctgttaag gcgaaccgtt ttcggcgtct gagtaacttt ctgtatcacc
      181 ggacggggag gcagtggtgc aacccactca ccagtcgctc gctcaaccgg cggtatcagc
      241 cacattccct gatacaatcc cagtccgtaa cgctgcggca caccatgttg ctgccagtcg
      301 ttcagtaact ggctgtcagc ctgaagacgt tgcagtgcag ttgttttcaa ttcagatgaa
      361 tatccgcgca tccggtgcca ggccgtcagg tgttccccca cactgcgaac cagtcgctgg
      421 ttattcataa aagacgccag catagccagc agaagaaaaa caccgacaat caaccccgtc
      481 tggcgacaaa gtctgtgcca gtgactgagt cctcgatgac gggg
//
LOCUS       DAZUCE010000619          513 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZUCE010000619 DAZUCE010000000
VERSION     DAZUCE010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.622"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..494
                     /locus_tag="ACHY03_005454"
     CDS             <1..494
                     /locus_tag="ACHY03_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFS3908523.1"
                     /translation="QPKKTTLRDKAMVNFAFDYLSSPGSLPFTTAATELSAIHGHSTS
                     QYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKA
                     ILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAERLLAGL
                     PAH"
BASE COUNT          156 a          122 c          115 g          120 t
ORIGIN      
        1 gccaacccaa gaaaacgacg ttgcgtgata aagccatggt gaattttgcc tttgattatt
       61 taagctcacc gggcagtctt ccattcacca cggcagccac ggagctttcc gcgattcatg
      121 gtcactcaac gtcgcaatat cgccttggag aattttatct tcatggtagc gacggtaaac
      181 cactggatta tacacaggcg agatactggt atgagcaatc agcggaacag gaaaatccac
      241 gcgcgcaaag taaactgggg tggatctacc tcaaaggtct gggggtcaaa cccgacaccc
      301 gtaaagcaat tctctggtat aaggaagcag ctgaacaagg gtatgctcat gctcaatata
      361 ctttaggttt gatctacaga aatggctcag gtattaatgt taaccattat gaatctcaaa
      421 aatggttaaa actggccgcc aaacaacatt acaaaaatgc ggaaagatta cttgccgggc
      481 ttcccgcaca ttaacgtcga ctgtcacccg aac
//
LOCUS       DAZUCE010000620          510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZUCE010000620 DAZUCE010000000
VERSION     DAZUCE010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.623"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            88..>510
                     /locus_tag="ACHY03_005455"
     CDS             88..>510
                     /locus_tag="ACHY03_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HFS3908524.1"
                     /translation="MSIIVNHVMSSDIVSGIFTDLLSYYKRYCDRDISIVESSNAIDN
                     ADVYHYHRPHLESRLKENSVVTIHHDLYDTDPWLDYDKFHPRYMEAKKILCLNSSQQN
                     YLNNKGLMNTEIIPHGYNSDIFCSVGAQKKIKNKVCIGF"
BASE COUNT          186 a           80 c           83 g          161 t
ORIGIN      
        1 actacagtgt agtaactgat gcgctggcag aatatattat cgacactaaa atacaagtat
       61 aaactttcca agtaacaagg cgttattatg tctataattg taaatcatgt tatgtcttct
      121 gatatagtaa gtggaatatt tacagatctt ctctcttatt acaaaagata ttgtgataga
      181 gatatatcaa ttgttgaaag tagcaacgct attgataatg ctgatgtcta tcactatcat
      241 cggcctcatt tagaaagcag acttaaggaa aactcagtag taacaataca tcatgacttg
      301 tatgatacag acccatggtt ggattatgat aagtttcatc cccgttatat ggaagcaaaa
      361 aaaatactgt gccttaactc ctctcagcaa aattatttaa acaataaagg cttaatgaat
      421 actgaaatta taccacatgg ttacaattct gacatctttt gctcagtagg ggcgcaaaaa
      481 aaaataaaaa ataaagtttg cattggtttt
//
LOCUS       DAZUCE010000621          510 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZUCE010000621 DAZUCE010000000
VERSION     DAZUCE010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.624"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
BASE COUNT          165 a          102 c          114 g          129 t
ORIGIN      
        1 gaagtgtacc acgctgagga aatcatcata tcgctgtttt ccgcccctgc gtggcgcatc
       61 acaggtcagg gaaataacac aacatatagt gtcatgagca gccgaatgaa gcactacatg
      121 ttgtacttac aggaggtggc gttgtgtggg ggagaggaag atccgtgacc aggggttttc
      181 ggatctctgg agcagaaaag aaaattattg ccagataatc acaaaaaatg gtggattatt
      241 gtgatgaagt gattattgca atcagaacaa taactggaat tgtaaagatc atattcatca
      301 ataaatagca gagtcacaga tctgctgaaa gccaaaccat gaagatacta cccgagtccg
      361 cttctggaag atccatttca aatatccgga tacacaggca actgtggcga cgattgtaat
      421 gacattaacc atatcggctt ccctgaagca ttggttatgg caaccacgtt atcaatatct
      481 ttccatcatt ttcagtaaat atcactaaca
//
LOCUS       DAZUCE010000622          504 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZUCE010000622 DAZUCE010000000
VERSION     DAZUCE010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.625"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>504)
                     /locus_tag="ACHY03_005456"
     CDS             complement(<1..>504)
                     /locus_tag="ACHY03_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFS3908525.1"
                     /translation="CVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGG
                     GFSGSMPLLAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPS
                     DVVSIDPKLLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFN
                     ITINTVNL"
BASE COUNT          137 a          131 c          134 g          102 t
ORIGIN      
        1 caggttgacg gtattgatgg tgatgttgaa gccatgttcg ctgaagtaca tcgccaggta
       61 agcgataccg agtgcgccga tgatcaacgt cagaatgcgg ctttcttcca gtcgctcgga
      121 tggtggggca tctttcggta gctgcttttg aaaatcagcc tcttccatga gtagtttcgg
      181 atcgatactc accacgtcag acggttttgg catcatcatg cgggtgataa atggcatcac
      241 cacaatcaac gccacagtga tgaaaatgtt aaaaccactg aacagagtat cgcccaccgg
      301 gatcagcccg gcgatatgct caaccgggtt gcccggtgtt gcagccaaca gaggcattga
      361 gccagagaaa ccgccacccc aggtgagaaa accaatgtag gcgcaggcaa tgagcaacgg
      421 ataatcagaa ccgggtactc ggcgggcgac ttcacgggca aacattgcgc cgacaaccaa
      481 accaaatccc cagttgatga caca
//
LOCUS       DAZUCE010000623          501 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZUCE010000623 DAZUCE010000000
VERSION     DAZUCE010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.626"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>501)
                     /locus_tag="ACHY03_005457"
     CDS             complement(<1..>501)
                     /locus_tag="ACHY03_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFS3908526.1"
                     /translation="AVAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSV
                     SINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDK
                     AIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDSSVNS
                     GDVLSSL"
BASE COUNT          155 a          111 c           87 g          148 t
ORIGIN      
        1 cagagaagat aacacatcac ctgaattcac tgagctatct gtcaatttca ggtattttgc
       61 tttaaacgga atagcatatt gaccttccgc tgttttataa ctctcaatat taaaaggtgc
      121 agaaacatcc aggacatcgc tgttatcacc taaatagaga tagtaaccca cagtatcgtt
      181 gctactggct atcgccttat cgttaccagt tattttatcg ttggtaatca acgagagttt
      241 tacaccagca acttctttcg ggcagctaat ttttaacatg ctgctggtta caccgactgc
      301 atcatcatag gtagaatatt tagctttacc ttcaaagaca gtggttggta cagtcggcat
      361 attaatactt actgaatcac cacccgcttc gaatttacac ggagattctg caatattccc
      421 tttaatagtt aatgttccgg tatcaacgtc tgcagcaaat actccagcgc tcataaaaaa
      481 agaagaaaag acagcgacag c
//
LOCUS       DAZUCE010000624          500 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZUCE010000624 DAZUCE010000000
VERSION     DAZUCE010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.627"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>500)
                     /locus_tag="ACHY03_005458"
     CDS             complement(<1..>500)
                     /locus_tag="ACHY03_005458"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3908527.1"
                     /translation="VSKKGKGSKTLNSKSGQIANFIANKIIFNYIPAVRTDQEAIDVV
                     GRMLSQRLRVLEDDETYLTALKTIKDLQQPILDSLSERIKEPLEQFLPGINSVSIEIP
                     DDTRRNSLRRDFEIIVDDGTPTSLAFKGDGVKSLAALGLLKDRVRTDGASIIAIEEPE
                     SHLHPAA"
BASE COUNT          142 a          101 c           83 g          174 t
ORIGIN      
        1 gctgctggat ggagatgtga ttcgggttct tcaatagcaa taattgaagc tccgtctgtt
       61 ctcacacgat cctttaataa gccaagtgca gctaaacttt ttaccccatc acctttaaat
      121 gctaaacttg ttggggttcc atcatcaaca atgatctcaa aatctcggcg taatgaattt
      181 cgtctggtat cgtcaggaat ctctatactt acagagttta ttccaggcaa aaattgttcc
      241 aaaggttctt tgatacgttc actcaatgaa tcaagaattg gttgctgcaa atcttttatt
      301 gttttaagag ccgtcaaata cgtttcatca tcctccagta ctcttaatct ttgagataac
      361 attctaccca caacatctat agcttcttga tcagtacgaa cggcgggtat atagttaaat
      421 attattttat tagcaataaa attagctatc tgtcctgatt tggaatttag agttttagaa
      481 cctttgcctt tcttcgatac
//
LOCUS       DAZUCE010000625          495 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZUCE010000625 DAZUCE010000000
VERSION     DAZUCE010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.628"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..394)
                     /locus_tag="ACHY03_005459"
     CDS             complement(<1..394)
                     /locus_tag="ACHY03_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS3908528.1"
                     /translation="METVFDALKAMGKASSVGLAERLDISREEVLNELWELKRNGVVD
                     KAGHIWFLAGEGESGVTEEQPEQSEVPDVLTGEVEQKVTADMMIEFIGQEGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQ"
     gene            complement(428..>495)
                     /locus_tag="ACHY03_005460"
     CDS             complement(428..>495)
                     /locus_tag="ACHY03_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS3908529.1"
                     /translation="GPTPIEWLKQEYERRKAAGFI"
BASE COUNT          108 a          157 c           91 g          139 t
ORIGIN      
        1 tctgatttac gcgtgccagg cgccccgttg ctgttacgac cgccaacgtg gaagcaacct
       61 tgcgaatgct aacaccgaac ttatccgcca gttcctcaca cgttttagcc ccctcctgac
      121 cgataaactc aatcatcatg tctgcggtaa ctttttgttc gacctccccg gtcagcacat
      181 ccggtacttc agactgttct ggctgctctt cggttacccc ggattcacct tcgccagcca
      241 gaaaccagat gtgacccgct ttatcaacaa cgccatttct tttgagttcc cacagttcgt
      301 tgagaacctc ttcacgactg atatcaagcc gctcagccag tccaacagag ctggcttttc
      361 ccattgcttt cagtgcgtca aaaacggttt ccattaaaat ttcctcctac aaaatcgctt
      421 ctcagattca aataaaacca gctgccttcc ggcgttcgta ttcctgtttc agccattcaa
      481 ttggcgttgg ccctt
//
LOCUS       DAZUCE010000626          492 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZUCE010000626 DAZUCE010000000
VERSION     DAZUCE010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.629"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..258
                     /locus_tag="ACHY03_005461"
     CDS             <1..258
                     /locus_tag="ACHY03_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908530.1"
                     /translation="SGPIIFSEDLPPQIRQPVCNAGEAKTAPVGERNLKEEIKRVEKR
                     IIMEVLEQQEGNRTRTALMLGISRRALMYKLQEYGIDPADV"
BASE COUNT          151 a          118 c           97 g          126 t
ORIGIN      
        1 tcaggcccga tcattttttc tgaggatctt ccgccgcaga ttcgtcagcc agtctgtaat
       61 gctggtgagg caaaaacagc ccctgtcggt gagcgtaatt taaaagagga aattaaacgc
      121 gtcgaaaaac gcatcattat ggaagtgctg gaacaacaag aaggaaaccg aacccgcact
      181 gctttaatgc tgggcatcag tcgccgtgca ttgatgtata aactccagga atacggtatc
      241 gatccggcgg atgtataacg ccaaaacttg ctatgcagaa atttgcacag tgcgcaattt
      301 tctgcatagc cgcttattct ccttatgaat ccccatctaa tttatccttt tctattcaat
      361 tatttaaata aaaacaccca ataatctcat tctggcactc cccttgctat tccctgactg
      421 tacccacaac ggtgtatgca agagggataa aaaatgaaaa caaaattgat gacattacaa
      481 gacgccaccg gc
//
LOCUS       DAZUCE010000627          486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZUCE010000627 DAZUCE010000000
VERSION     DAZUCE010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.630"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>486
                     /locus_tag="ACHY03_005462"
     CDS             <1..>486
                     /locus_tag="ACHY03_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908531.1"
                     /translation="RDFDHLSTLLHLFFEREDICQKLNIIDQNNITGWEVWFQVEFAN
                     MLCSTDHEWWREQALSCDMRKKPERPTLRTDFLLRKKGWAQDSYIALEIKQNRDATSC
                     VKNMIADLEKSAKIKRSELDLRSFWTLGITPMIDRERLDSLIDCYLDEKFYLTKSRKK
                     H"
BASE COUNT          160 a          102 c           97 g          127 t
ORIGIN      
        1 accgtgactt cgatcatctc tcaacgctat tacatctctt ttttgaacgt gaggatattt
       61 gccaaaagct taatattatt gaccaaaaca atatcaccgg ttgggaagtg tggttccagg
      121 tagagtttgc taatatgctc tgctcaacag accatgaatg gtggcgtgaa caagcacttt
      181 cctgtgatat gcgcaaaaaa cctgagcgcc cgacactacg cacagatttc ttactacgta
      241 aaaaaggctg ggcacaagat agctatatcg cgcttgaaat aaaacaaaac agagacgcga
      301 ccagttgtgt taaaaatatg atcgcagact tagaaaaaag cgcaaaaata aaacgttccg
      361 aactcgattt acgcagtttc tggaccttgg gtatcacccc tatgatagac agggaaaggc
      421 tggactctct catcgattgc tatttagatg agaaatttta tcttactaaa tctcgtaaaa
      481 aacacg
//
LOCUS       DAZUCE010000628          486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZUCE010000628 DAZUCE010000000
VERSION     DAZUCE010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.631"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..391)
                     /locus_tag="ACHY03_005463"
     CDS             complement(<1..391)
                     /locus_tag="ACHY03_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbJ"
                     /protein_id="HFS3908532.1"
                     /translation="MYPTDRRQLNTERQIYLDKQFFVDVFSIPACIRNTNGDFIGYNE
                     KFSKEFIGSLDIKEWFYSLPVQVATSFLREELDAMSLPSSMNKIQSVAIGDKLWLVQF
                     IPLIYGEVVNVLWLFFCKNSNVIVDYCR"
BASE COUNT          158 a          100 c           82 g          146 t
ORIGIN      
        1 ctctacaata atccactatc acattagaat ttttgcagaa aaaaagccat aaaacattaa
       61 ccacctcccc atatattaat ggtataaatt gtaccagcca gagtttatca cctatagcca
      121 cgctctgtat tttattcatg gacgaaggca aagacatagc atccagttct tctcttaaga
      181 acgatgtagc tacttgtaca ggaagggagt aaaaccactc tttgatatca agactaccta
      241 taaattcttt agagaatttt tcattgtagc cgataaaatc tccattagta tttcgaatgc
      301 aagcagggat tgaaaaaaca tcgacaaaaa actgtttatc caaatatatt tgacgttcag
      361 tattaagttg tctacgatcc gtcggataca taggaacctc ctctcaaagg attctatgga
      421 cagtcgatgc ggggaggtcg ctctccctgc atcggcgatt tttaatatct gttttaacgt
      481 ggcatt
//
LOCUS       DAZUCE010000629          486 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZUCE010000629 DAZUCE010000000
VERSION     DAZUCE010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.632"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>486
                     /locus_tag="ACHY03_005464"
     CDS             <1..>486
                     /locus_tag="ACHY03_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFS3908533.1"
                     /translation="NKIMEAADISRPDADKLLAASRGEFVEGISDPNDSKWVKGIETC
                     DSVNQNQQETEQNGQKAEQNSPNALQNEPETKQPEPVVQQEEEKVCTACNQTGGGNCP
                     DCGAVMGDATYQETFNEESQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETGETA
                     N"
BASE COUNT          172 a          102 c          136 g           76 t
ORIGIN      
        1 taaataaaat tatggaggca gcagacatca gtcgaccaga tgccgacaaa ttgcttgccg
       61 cttctcgtgg tgagtttgtt gaagggatta gcgacccgaa tgattcgaaa tgggttaagg
      121 ggattgaaac ctgcgattct gtgaaccaga accagcaaga aacggaacag aacggtcaga
      181 aagcggaaca aaacagccca aatgcgttac aaaacgagcc agaaacgaaa caacctgaac
      241 cagtagtgca acaggaagag gaaaaagttt gtaccgcatg caatcagact ggtggtggta
      301 actgccctga ttgtggcgca gtgatggggg acgcaaccta ccaggagaca ttcaacgaag
      361 aaagccagga tgaagcccgg gaaaaagatc cggaggaaat ggaaagtgcc ggactcccga
      421 acaaggagtg caccgaaggc gatcaacatg ccaatggcaa taatgaaaca ggcgagacag
      481 caaatc
//
LOCUS       DAZUCE010000630          479 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZUCE010000630 DAZUCE010000000
VERSION     DAZUCE010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.633"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..130)
                     /locus_tag="ACHY03_005465"
     CDS             complement(<1..130)
                     /locus_tag="ACHY03_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908534.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYN"
     gene            148..339
                     /gene="yfgG"
                     /locus_tag="ACHY03_005466"
     CDS             148..339
                     /gene="yfgG"
                     /locus_tag="ACHY03_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFS3908535.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          124 a          118 c           86 g          151 t
ORIGIN      
        1 cattgtaaat aattaaatta acttccataa cattaaaata tgtatccact gacgcttttt
       61 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
      121 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
      181 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
      241 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
      301 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc tccactttca
      361 cagaacataa cggcacttcg ctgtcggatg cttttgctct ttgggattat caaagcggca
      421 gatattcttt catcttaaat tttacgtctt tgtcctgact gatgtttatc ctgtttggc
//
LOCUS       DAZUCE010000631          477 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZUCE010000631 DAZUCE010000000
VERSION     DAZUCE010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.634"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>477)
                     /locus_tag="ACHY03_005467"
     CDS             complement(<1..>477)
                     /locus_tag="ACHY03_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908536.1"
                     /translation="NNKLQPAEIYKESQQTAKLEFFCDEFLKISQSRYGLSTSTDSIA
                     NLLAFFTKASDAIDSIKTQKIDVHSYGFVPIRHFVEHVITYKNEFAADYEPYTLTFTR
                     GENNEGILSVESKEGFISQRTINLNEYETAINIINEHVIKENIHNTVQSPTEKDIS"
BASE COUNT          124 a           74 c           88 g          191 t
ORIGIN      
        1 tttgaaatgt ctttttctgt cggtgactgg accgtgttgt ggatattttc ttttataaca
       61 tgttcattga tgatgttaat tgcagtttca tattcattta agtttattgt tctttgtgaa
      121 ataaaccctt ctttgctttc gacactcaaa ataccttcgt tgttttcacc tcgggtaaag
      181 gtaagggtat agggttcata atccgctgca aattcatttt tatacgttat gacatgttcg
      241 acaaaatgtc ttattggtac gaaaccataa ctgtgaacgt caattttttg ggtcttgata
      301 ctatcaattg catctgaggc tttcgtgaaa aaagccaata aattagctat gctgtctgtt
      361 gaggttgata acccgtatct actttgagat atttttagaa actcatcgca aaaaaactca
      421 agtttggcgg tttgttgtga ttctttgtaa atttctgctg gttgcaattt attattt
//
LOCUS       DAZUCE010000632          473 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZUCE010000632 DAZUCE010000000
VERSION     DAZUCE010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.635"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..313
                     /locus_tag="ACHY03_005468"
     CDS             <1..313
                     /locus_tag="ACHY03_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFS3908537.1"
                     /translation="FPELYDDSHPEALARNTQKIFRWVEKDTSDAVEKIQALLPAIEK
                     AMPPLLVARMRSHSSEYYREIVERRDRLVKDVDDFVAVAIAWGTLTNSGGQPGNAVVV
                     H"
     gene            382..>473
                     /locus_tag="ACHY03_005469"
     CDS             382..>473
                     /locus_tag="ACHY03_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908538.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIAT"
BASE COUNT          115 a          107 c          140 g          111 t
ORIGIN      
        1 gttcccggaa ctgtacgacg acagccatcc ggaagccctg gctcgtaata cccagaaaat
       61 tttccgctgg gtggagaagg acacgtctga tgctgttgaa aaaattcagg cgctgttacc
      121 ggcgatcgaa aaggcgatgc cgcctttgct ggtggcccgt atgcgcagtc acagttctga
      181 atattaccgg gagatcgtcg aacgacggga tcggctggtg aaagatgtgg atgattttgt
      241 cgctgtagcg atcgcctggg gcaccctgac taacagtggt ggtcagcctg gtaatgcagt
      301 tgttgtgcat tgaccaacaa tatttatacc ggatttcttc cggaagttcg tgggtaaagt
      361 tcggtatcag aagaggtgag tatggctaat gcctggctca gattgtggca tgacatgcca
      421 aatgacccca agtggcgaac gattgccagg gtatcaggac agccaatcgc aac
//
LOCUS       DAZUCE010000633          472 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZUCE010000633 DAZUCE010000000
VERSION     DAZUCE010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.636"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>472
                     /locus_tag="ACHY03_005470"
     rRNA            <1..>472
                     /locus_tag="ACHY03_005470"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          118 a          113 c          142 g           99 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg ga
//
LOCUS       DAZUCE010000634          471 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZUCE010000634 DAZUCE010000000
VERSION     DAZUCE010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.637"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            15..>471
                     /locus_tag="ACHY03_005471"
     CDS             15..>471
                     /locus_tag="ACHY03_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFS3908539.1"
                     /translation="MLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREGEYLPSER
                     ELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGIVKDFLST
                     ADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEV"
BASE COUNT          157 a          100 c          102 g          112 t
ORIGIN      
        1 acaacaaaaa aaccgtgtta cctgggctaa caacgaccag aaagcccatt gttacaaaaa
       61 aactttcaga aatcgttgcc gaagagcttg aaagaatgat ccgccaggag gagataaggg
      121 aaggtgaata cctgccttct gaacgtgaac ttatggcatt ctttggcgtt ggaagaccat
      181 ctgtcagaga ggctttatca tctctcagac ataaagggct aatccggtat aacaagggag
      241 aaaaagccag tgtctgccgt ccatcaccag aaaccatcat taatgggtta tcaggcattg
      301 ttaaagattt tttatccaca gctgatggca tcaaggattt tgaaagacta cgcatctttt
      361 ttgaaaccaa ccttgtaaga tatgcagcaa aaaatgcaac gcctgaggat attcacaggc
      421 ttgagaaggt gctcagtatc catcataact cactgagaac ccccgaagtg t
//
LOCUS       DAZUCE010000635          470 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZUCE010000635 DAZUCE010000000
VERSION     DAZUCE010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.638"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>470
                     /locus_tag="ACHY03_005472"
     CDS             <1..>470
                     /locus_tag="ACHY03_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3908540.1"
                     /translation="SGAETALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVETSSK
                     GDRNPSSVRLLIQLERNGNWVTEKDVTINGKTTSQYLTSVILNNLPERPFNIRMVRVT
                     ADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMVVNYHIRG"
BASE COUNT          109 a          126 c          147 g           88 t
ORIGIN      
        1 tccggggcag aaaccgcact gggcgtggag gtgacgaagg caaagccggt gacgcgcacc
       61 atcacgtcag cgaacattga ccgtctgcgg gtcaccttcg gggtgcagtc actggtggag
      121 accagctcaa agggtgaccg taatccctct tctgtccggc tgctgattca gcttgagcgt
      181 aacggtaact gggtgacgga gaaggatgtc accattaacg gcaagaccac ctcgcagtac
      241 ctgacgtcgg tgattctgaa taatctccct gagcgcccct ttaacatccg gatggtcagg
      301 gtgacggcgg acagtaccac ggaccagctg cagaacagaa cgctgtggtc gtcatacacc
      361 gaaatcatcg atgtgaaaca gtgctacccg aacacggcca ttgtggggct gcaggtggat
      421 gcggagcagt tcggtggcca gcagatggtg gtgaactacc atatccgcgg
//
LOCUS       DAZUCE010000636          469 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZUCE010000636 DAZUCE010000000
VERSION     DAZUCE010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.639"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            301..453
                     /gene="mokC"
                     /locus_tag="ACHY03_005473"
     CDS             301..453
                     /gene="mokC"
                     /locus_tag="ACHY03_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFS3908541.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT          146 a           95 c          124 g          104 t
ORIGIN      
        1 aatccagctt gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc
       61 attaaaaaat tacgccataa aaatgcgagg cgtcttccgg attagaatcg ataaaacaga
      121 atgggttaaa gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt
      181 agctgaacgg cagatagaga aaagccccga gtgatatttt accatcaacc cgaggccccc
      241 tatatgctga acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca
      301 atgaagcagc ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg
      361 gcggcgctgg taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag
      421 gttgctgttt tcacggctta cgaatccgag taagagcaac ggcggggag
//
LOCUS       DAZUCE010000637          465 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZUCE010000637 DAZUCE010000000
VERSION     DAZUCE010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.640"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..161)
                     /locus_tag="ACHY03_005474"
     CDS             complement(<1..161)
                     /locus_tag="ACHY03_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908542.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGT"
     gene            complement(247..453)
                     /locus_tag="ACHY03_005475"
     CDS             complement(247..453)
                     /locus_tag="ACHY03_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3908543.1"
                     /translation="MNTPVSLMADQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
BASE COUNT          109 a          114 c          126 g          116 t
ORIGIN      
        1 gtgccttttt ctggcagaaa aaagtgtgcc agggtcaggc ggtgataacc atgaagatgc
       61 ggagacccca ttccatgagc gacgataagc ctcgtcacgc ttgtcatcca tttctgatgc
      121 tggatgagca gcagctcaca ccggttttca agtttttgca taatattctc tcctttagcg
      181 cgcagcaaaa tgcctccggg ggccggatca tgcctttgct gcggttgaac ggataacggt
      241 caggaattac ggacgggact gtgcaatacg cgcctgcagc caggtttcca cttcactttt
      301 cagccagcgg gaactgcggc ccagtttgat gggggcagga aaggcaccat ccttgatgag
      361 cttgtaaaac catttatcgg tcaggccggt cagttgagtg ataaatgcca tgtcgaccat
      421 ctggtcagcc atcagtgaaa ctggggtatt catgaaaatg ttcct
//
LOCUS       DAZUCE010000638          465 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZUCE010000638 DAZUCE010000000
VERSION     DAZUCE010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.641"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..161)
                     /locus_tag="ACHY03_005476"
     CDS             complement(<1..161)
                     /locus_tag="ACHY03_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908544.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGT"
     gene            complement(247..453)
                     /locus_tag="ACHY03_005477"
     CDS             complement(247..453)
                     /locus_tag="ACHY03_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFS3908545.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          107 a          114 c          129 g          115 t
ORIGIN      
        1 gtgccttttt ctggcagaaa aaagtgtgcc agggtcaggc ggtgataacc atgaagatgc
       61 ggagacccca ttccatgagc gacgataagc ctcgtcacgc ttgtcatcca tttctgatgc
      121 tggatgagca gcaggtcaca cctgttttca agttgttgca taatattctc tcctttagcg
      181 cgcagcaaaa tgcctccggg ggccggatca tgcctttgct gcggtggaac ggataacggt
      241 caggaattac ggacgggact gtgcaatacg cgcctgcagc caggcttcca cttcgctttt
      301 cagccagcgg gagctacggc ccagtttgat gggggcagga aaggcaccat ccttgatgag
      361 cttgtaaaac catttatcgg tcaggccggt cagttgagtg ataaatgcca tgtcgaccat
      421 ctggtcatcc atcagtgaaa ctggggtatt catgagaatg ttcct
//
LOCUS       DAZUCE010000639          463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZUCE010000639 DAZUCE010000000
VERSION     DAZUCE010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.642"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            1..463
                     /locus_tag="ACHY03_005478"
                     /pseudo
     CDS             1..463
                     /locus_tag="ACHY03_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          147 a           61 c           96 g          159 t
ORIGIN      
        1 aatacgccgg gaatatctga tttagattct ggtattgatt tgtctggtat atcttttatt
       61 cagagtgaaa ttaataaaac ttatggatta aaatatgctc ctgtagatgg cgatggatat
      121 tgtctgttaa gagctatact ggttttaaaa caacatgatt attcatgggc gctgggcagt
      181 cataagacac aaaaggaagt atacgaagag tttattaaaa ttgttgataa acaaacgatc
      241 gagctcttgt tgatgcggca ttctatgatc tcagggaaga tgttaagaca ttatttggcg
      301 ttgatctgca atctgacaac aaaattcagg gtgtgggtag tcatatgtca tggagctttc
      361 tgttttttaa aaaacaattc attgatagtt gcttgaataa cgaaaaatgt atcctgcatt
      421 tacccgagtt tatatttaat gataacaaga atttgcttgc ttt
//
LOCUS       DAZUCE010000640          463 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZUCE010000640 DAZUCE010000000
VERSION     DAZUCE010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.643"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            164..>463
                     /locus_tag="ACHY03_005479"
     CDS             164..>463
                     /locus_tag="ACHY03_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFS3908546.1"
                     /translation="MTFTITQDRQLASLTVTKEEKEILFAVKSKAGGGLFIRVRYGSD
                     NKSWIYRYRIAQKPAKLTLGSYPAMGLAQARKAHADAIELVRKGIDPRHIRKNEKQ"
BASE COUNT          170 a           81 c           86 g          126 t
ORIGIN      
        1 agcgagacca gtgattcaag atgttcacag ctgtttccgc atacaataaa aaaatatata
       61 aatcaacaaa atacacataa atgcagtatc aagatgtttc gccttgtttc tgatgatatc
      121 atgttaaatg tgtaccccac tgtgtaccca aaaggctgaa gatatgactt ttactattac
      181 acaagacaga caattggctt cattgaccgt tactaaagaa gaaaaagaaa tcttatttgc
      241 tgtaaaatct aaagctgggg gaggactctt tatacgcgtt cgttatggta gcgacaataa
      301 aagctggatt tatcgttatc gaatagctca aaagccagct aaattaacac taggtagtta
      361 ccctgccatg ggattagctc aagctcgtaa agctcatgcg gatgcaattg aattagtaag
      421 aaaaggtata gatcctcgtc atattagaaa aaatgaaaaa caa
//
LOCUS       DAZUCE010000641          459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZUCE010000641 DAZUCE010000000
VERSION     DAZUCE010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.644"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>459
                     /locus_tag="ACHY03_005480"
     CDS             <1..>459
                     /locus_tag="ACHY03_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HFS3908547.1"
                     /translation="KYVNLSFDIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLVAH
                     GANNFYESLSPLFFNEYDSGPIRELYRVIAKKMGGDINSADYAYSIVDFSITAYGGYS
                     GMKIVPKYNRLIRPSLGNRPGTGRLFHYTSVDFKNKFSLKPTPLKIIQISS"
BASE COUNT          150 a           70 c           92 g          147 t
ORIGIN      
        1 aaaatatgtg aacttaagtt tcgatattgt tgcatctgta ggacaggtcg taggcggggt
       61 tggcgcgttg aaatttggta aggtagtgca ttcaaaccga ataaaaggta taggcgtcac
      121 attagttgct catggggcga ataattttta tgaaagctta tcacctttat tttttaacga
      181 gtatgattca ggtccaatac gtgaactcta cagagtaata gcaaaaaaaa tgggaggtga
      241 tattaattct gctgattatg catacagcat cgtggacttt tcgattactg catatggtgg
      301 atattcaggc atgaaaatcg tacctaagta taatagattg attcgtcctt cattaggaaa
      361 tagacctggt acaggacgat tatttcatta cacatcagtt gattttaaaa ataaattctc
      421 attaaaacct acccctttaa aaatcataca aattagttc
//
LOCUS       DAZUCE010000642          459 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZUCE010000642 DAZUCE010000000
VERSION     DAZUCE010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.645"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            207..>459
                     /locus_tag="ACHY03_005481"
     CDS             207..>459
                     /locus_tag="ACHY03_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFS3908548.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDT"
BASE COUNT          139 a           73 c           97 g          150 t
ORIGIN      
        1 tcacttaatg tgattccgga taattcgaaa ggcgctgtgc ttttcggatt attattatct
       61 gattaagata tttgtcacgc cagccttact ggtttataaa tgtcaatcat gattcttata
      121 aattatttat tgctgctttg gttaccatta cttcgtaata agtaatgtat ttttgatggt
      181 ttaaactatt tttaagggac ggcattatgc gagggaaaat acctaaaacg gaattgctgg
      241 tgacttttga ggtcgtcgca cgtcatgaaa gttataccag agcggcagag gagttagcgt
      301 taacccaaag tgcagtattc agacaagtaa gtgcgctgga ggaattttta cgtactccgt
      361 tattttcaca ctctaaaaaa cgcatttttc ttaatgatgc agggaagtat tacctgggga
      421 tagtaaaaga aacattaaat aagctggaac gcgatacca
//
LOCUS       DAZUCE010000643          453 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZUCE010000643 DAZUCE010000000
VERSION     DAZUCE010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.646"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            2..>453
                     /locus_tag="ACHY03_005482"
     CDS             2..>453
                     /locus_tag="ACHY03_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3908549.1"
                     /translation="MNDEVRFNSSSNKLTSLLTSEEVILTYPAGKCLKLLLDNAYSVV
                     SHNQFFEKVWSDSSAEVATNTLYQNISMIRRSFREISSATIFNDVIRTVPRKGFKINE
                     EISITVLDNNHNENESVESEENGTHTENINKNHIAKGIFTTKNTLLIV"
BASE COUNT          170 a           79 c           79 g          125 t
ORIGIN      
        1 tataaatgat gaagtaagat tcaactcatc atctaataag ttaacatcac tccttacatc
       61 agaggaagtg atactcactt atcctgcggg aaaatgctta aagttacttt tagataacgc
      121 ttatagtgtc gtgtcacaca atcaattctt tgaaaaagtc tggagcgata gcagtgccga
      181 agtcgcaacc aacactttat atcaaaatat atcgatgata agacgttctt ttcgtgaaat
      241 cagctcagcg acgatattta atgatgttat cagaacggtg ccccgtaaag gcttcaaaat
      301 caatgaagaa atcagcataa ctgttctcga caataatcac aatgagaatg aaagtgttga
      361 gagtgaagag aatggtaccc acactgaaaa tataaataaa aatcacattg caaaaggtat
      421 atttaccaca aagaatacat tgcttatagt ttg
//
LOCUS       DAZUCE010000644          450 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZUCE010000644 DAZUCE010000000
VERSION     DAZUCE010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.647"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..68
                     /locus_tag="ACHY03_005483"
     CDS             <1..68
                     /locus_tag="ACHY03_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFS3908550.1"
                     /translation="GPTPIERLKQEYERRKAAGFI"
     gene            102..>450
                     /locus_tag="ACHY03_005484"
     CDS             102..>450
                     /locus_tag="ACHY03_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFS3908551.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVTELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQ"
BASE COUNT          127 a           98 c          133 g           92 t
ORIGIN      
        1 aagggccaac gccaattgaa cggctgaaac aggaatacga acgccggaag gcagctggtt
       61 ttatttgaat ctgagaaacg attttgtcgg aggaaatttt aatggaaacc gtatttgacg
      121 cactgaaagc actgaaaaaa gcctcttcac aggtagtggc atcgcgcctt ggaatcagcc
      181 gtgaagatgc tgttaccgaa ctctggaaac tgaagcgccg tggtgaagcg gataacaagg
      241 gttcgatgtg gtggctgatt caggctggtg aaagtgaacc agtgtcaccg gtaccgaaag
      301 taacagcgca aatgctgact gaggcgattg aacaacatgg cccacaaacg gcggatgagc
      361 tggcactgat gttcgggatt acctcccgcc gggcgaattc atcgctggcc atggcaatca
      421 gcaaagggcg tctgattcgc gtgaatcagg
//
LOCUS       DAZUCE010000645          443 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZUCE010000645 DAZUCE010000000
VERSION     DAZUCE010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.648"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            94..369
                     /locus_tag="ACHY03_005485"
     CDS             94..369
                     /locus_tag="ACHY03_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFS3908552.1"
                     /translation="MNLGEYLHHSGITQKHFAEIVGVTQGMVSHVITGRAKLTGEKIL
                     RWCEATGWIVTPHEIDSSTYPNPTDGLPVEYQANTQPEAGVIHENQA"
     gene            353..>443
                     /locus_tag="ACHY03_005486"
     CDS             353..>443
                     /locus_tag="ACHY03_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS3908553.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIAR"
BASE COUNT          142 a           89 c          106 g          106 t
ORIGIN      
        1 taagattact aatctctttg aaatagtgat gctatttact tttaaaaata acaatgctat
       61 taatgatcgt gatgacatca catgaggtga acaatgaatc ttggagaata tttgcatcat
      121 tccggtataa ctcagaagca ttttgctgaa attgttgggg taacccaagg gatggtaagc
      181 catgtcatta ccggacgggc gaaacttacg ggggaaaaaa ttttacgctg gtgcgaagca
      241 acaggctgga tagtaactcc gcacgagatt gatagcagta cttatcccaa cccaaccgac
      301 ggcttacctg tcgagtatca ggctaacaca caaccagagg cgggggtgat tcatgaaaat
      361 caagcatgaa cacatccgca tggcaatgaa tgcctgggcg tatccagacg gtgagaaagt
      421 tccagcagct gaaatagccc gga
//
LOCUS       DAZUCE010000646          443 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZUCE010000646 DAZUCE010000000
VERSION     DAZUCE010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.649"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
BASE COUNT          136 a           85 c           75 g          147 t
ORIGIN      
        1 tctgttcgac atcatttcag aaatccgaat aaaacaacaa agttggtgag ttacaattaa
       61 tttaaatctt gttaattgat taacaaagca tcatcattac tgcatcttat tctctatatc
      121 ttttacgccg ggatagattc tttatggtca ttacgggcag gcaccatttc attatattac
      181 catttacatt cactcttgta acccttaaga gttatataaa aggcaatagt tcttgctcat
      241 ggtaatactg agatcggatt aaccgtgagg ttagctatat agagagagct ctttggcttt
      301 gcgacaatta gcttaagcag ccacattatg tttggcttac cgttgaaagg tagaaagaag
      361 agcaaaatgg tttacaaggt ttcattccca tctaatagca attaccccta tacatcatcc
      421 cttaacgtac tctgggcttc atg
//
LOCUS       DAZUCE010000647          443 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZUCE010000647 DAZUCE010000000
VERSION     DAZUCE010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.650"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..211
                     /locus_tag="ACHY03_005487"
     CDS             <1..211
                     /locus_tag="ACHY03_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFS3908554.1"
                     /translation="FIERVNDYIERAGYNMIPVGECSNGHITLLYGENNVIYGGLDDY
                     LWKYGDNLSSSIDILINGKEAVEIP"
     gene            273..>443
                     /locus_tag="ACHY03_005488"
     CDS             273..>443
                     /locus_tag="ACHY03_005488"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HFS3908555.1"
                     /translation="MSDTSSNRITFERDSQYRITGVSHTDGIRLKLTYHASGYLKAIH
                     RTGNGIQTQGKII"
BASE COUNT          130 a           92 c          113 g          108 t
ORIGIN      
        1 ttttatagaa agagtcaatg attatattga gagagctggt tacaatatga ttcctgttgg
       61 cgagtgcagt aatggtcata tcacattatt atatggggaa aataatgtga tatatggcgg
      121 cttggatgat tatctttgga agtatgggga taatttatct tcatcaattg atatattgat
      181 taatggcaag gaggcagttg aaattccgta aggccaactt gatatgacac ctggcacaca
      241 accccgacga ccggatgtat atgctgggcg ggatgagtga cacctccagc aaccgcatca
      301 cgtttgagcg cgacagccag taccggatca caggtgtcag tcacaccgac gggatccggc
      361 ttaaactgac gtaccacgcc agcggctacc tgaaagccat tcaccgcacg ggtaacggca
      421 tacagacgca gggaaagatc atc
//
LOCUS       DAZUCE010000648          438 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZUCE010000648 DAZUCE010000000
VERSION     DAZUCE010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.651"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            159..>438
                     /gene="gadE"
                     /locus_tag="ACHY03_005489"
     CDS             159..>438
                     /gene="gadE"
                     /locus_tag="ACHY03_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFS3908556.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKE"
BASE COUNT          158 a           65 c           85 g          130 t
ORIGIN      
        1 tggataaatc tgaagtattg attagtgtta atagacgtat tagttcacga agggcaaagt
       61 tcttataggc gtttactata ttgaacaacg attcggacaa ggatgtaaat aatgaaaagg
      121 atgacatgtt cgaaacgata acggctaagg agcaagttat gatttttctc atgacgaaag
      181 attcttttct tttacagggc ttttggcagt tgaaagataa tcacgaaatg ataaaaatca
      241 attccctgtc agagatcaaa aaagtaggca ataaaccctt caaggttatc attgatacct
      301 atcacaatca tatccttgat gaagaagcga ttaaatttct ggagaaatta gatgccgaga
      361 gaattattgt tttggcacct tatcacatca gtaaactaaa agctaaagcg cctatttatt
      421 ttgttagccg caaagaaa
//
LOCUS       DAZUCE010000649          435 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZUCE010000649 DAZUCE010000000
VERSION     DAZUCE010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.652"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            32..163
                     /locus_tag="ACHY03_005490"
     CDS             32..163
                     /locus_tag="ACHY03_005490"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFS3908557.1"
                     /translation="MTYENNKKTHQQHIEILTAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(199..>435)
                     /locus_tag="ACHY03_005491"
     CDS             complement(199..>435)
                     /locus_tag="ACHY03_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFS3908558.1"
                     /translation="NVTSCFPPVFLLHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLY
                     EKGGHGFSVQWATDLPVGGWIKLFMDWMYSHGFK"
BASE COUNT          166 a           94 c           70 g          105 t
ORIGIN      
        1 gataatggcc aaacctctgg aaaagaataa aatgacatat gaaaataaca aaaaaaccca
       61 tcagcagcat attgaaatac ttacggccat aaaagagcat gattgtgata aagctgaaca
      121 gctaatgtat aatcaccttt ataaacgaat tgaagcatca tagttactcg acgcttcacc
      181 aggtagtcac cataaatatt atttaaatcc atgagaatac atccagtcca taaacagttt
      241 tatccatcct ccaacaggaa gatctgtggc ccactgaaca ctaaagccat gccctccttt
      301 ctcatagaga tgcatttcag cagaaacgcc tttctctatt aggccacggt acatgagcag
      361 actgttttca attgacacaa gatcatcatc acaacaatgg agtaaaaaca caggaggaaa
      421 acatgatgtg acatt
//
LOCUS       DAZUCE010000650          435 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZUCE010000650 DAZUCE010000000
VERSION     DAZUCE010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.653"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..390
                     /locus_tag="ACHY03_005492"
     CDS             <1..390
                     /locus_tag="ACHY03_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908559.1"
                     /translation="GLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGVLLS
                     ELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKSHAE
                     LENWAVARKREKRELELMIDEYLLYAC"
BASE COUNT          105 a          117 c          108 g          105 t
ORIGIN      
        1 ggactctatc aggtcgtcaa cccgggaaca ccaccgttca ttcctgccat tcaggaaggg
       61 ttaatgacca gcattcagac ccatgaaatc atgctgctta cacattttaa tctgggcggc
      121 gttttactca gtgagctaca tcgtctgggt gaaaaccggc tggcaaacag gctgaacagc
      181 ctgctccggc gtttcgacga tcgtgatctc taccacacgc tgatatggct gtgctggtat
      241 gatctgatgt gtgcccactc catgcagccc tggactgaag agttaaagca taaatcacat
      301 gcagagcttg aaaactgggc tgtggcgcgc aaacgggaaa agcgcgagct ggaactgatg
      361 attgacgagt acctgttata cgcctgttga ttcataacgc tcccgggcct ccgcacactt
      421 acttttcctc cgtca
//
LOCUS       DAZUCE010000651          434 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZUCE010000651 DAZUCE010000000
VERSION     DAZUCE010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.654"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>434
                     /locus_tag="ACHY03_005493"
     CDS             <1..>434
                     /locus_tag="ACHY03_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908560.1"
                     /translation="PALLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHI
                     ALEAHTFDSVKFILTTALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFF
                     LMACSACAPLIALQYDISPSLMWWGGLLYWLAALVTLLWAAS"
BASE COUNT           90 a          108 c          113 g          123 t
ORIGIN      
        1 ccggccttac tggctggcta tatcaccgaa cgcataaccc ggttgttcca tattcgctgg
       61 ctggcaggcg tatttctgac gattgccgga atgatcatca gcttcatgtg gggacttgat
      121 ggtaaacata tcgcgctgga ggctcacacc tttgactctg tgaaatttat tctgaccacc
      181 gctctcgccg gtggtctgct ggctgttccc ctgcagatca aaaacattca gcagaacggg
      241 atcacaccag aagatatcag caaggaaatt aacgggtatt actgctgttt ttatactgcc
      301 tttttcctta tggcgtgttc tgcatgcgca ccattgatcg cgttacagta cgatatttca
      361 ccgtcactga tgtggtgggg cgggttgttg tactggctgg ctgcattagt gacgctgcta
      421 tgggcggcca gcca
//
LOCUS       DAZUCE010000652          433 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZUCE010000652 DAZUCE010000000
VERSION     DAZUCE010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.655"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
BASE COUNT          138 a           65 c           96 g          134 t
ORIGIN      
        1 ttgcatttga ttatataaaa gacatgattg taaatgtttc aagaactgaa agattggatg
       61 gttattttcg tgtgaatgat gatgaagata atttattctt agaatggatt gtaaaagatg
      121 gttatttaga ggaaataatt ttgtctggtt cataaattca tcttctttgt tgagaagatt
      181 aactgcctag gtgaaattat cctctatgac cgggacgctg caggccacct ggcagaagtc
      241 atcacgcagg aagacaagtc gctgtatccc tttgaaaaat ccggcaggct aatgggcatc
      301 ttcagcccag gcggcacatc acagcgaatt tgaaggcaaa tgcgaaaatt agtgatcttg
      361 aagttcatac agtattggta aaaacaggcg aagcttttcc aatgtgtaag aattttcaaa
      421 tttcaatatt tgg
//
LOCUS       DAZUCE010000653          425 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZUCE010000653 DAZUCE010000000
VERSION     DAZUCE010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.656"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>425)
                     /locus_tag="ACHY03_005494"
     CDS             complement(<1..>425)
                     /locus_tag="ACHY03_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFS3908561.1"
                     /translation="IVGRSGSGKSTLTKLLQRFYIPETGQILIDGHDLSLADPEWLRR
                     QIGVVLQENILLNRSIIDNITLASPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGE
                     QGVGLSGGQRQRIAIARALVTNPRILIFDEATSALDYES"
BASE COUNT          126 a           98 c           78 g          123 t
ORIGIN      
        1 cgactcataa tcaagagcac tggttgcttc atcaaaaata agaattcgag gatttgtaac
       61 aagagcccgg gctattgcga tccgttggcg ctgccctcct gaaagaccaa cgccctgttc
      121 tccaacaata gtattgtacc cttcttttag ttctgtaata aaatcatggg cacctgcaag
      181 tctggctgcc tcaatagcct gttccataga tacagccgga gaagctaatg taatattatc
      241 gataatacta cgatttaata gtatattttc ctgtaataca acaccaatct ggcgtcgtaa
      301 ccattctgga tctgcaagtg ataaatcatg cccatcaatt aaaatctgtc cggtctctgg
      361 tatataaaag cgctgaagca gttttgttaa tgtacttttt cctgaaccag aacggccaac
      421 tatac
//
LOCUS       DAZUCE010000654          424 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZUCE010000654 DAZUCE010000000
VERSION     DAZUCE010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.657"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>424
                     /locus_tag="ACHY03_005495"
     CDS             <1..>424
                     /locus_tag="ACHY03_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3908562.1"
                     /translation="QGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVT
                     LPETGTSTVNLINGSGKPVRVDITAHPAPDRIQVSALPDGVETYGVWGLSLPSLRRRL
                     FRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPLS"
BASE COUNT           86 a          137 c          130 g           71 t
ORIGIN      
        1 acaggggctg cggcacacgc ccggtgacat cattgaaatc tgtgataacg actacgccgg
       61 gaccctgacc ggcggacgca tcctgtccat cgatgccgcc agccgcacac tgacgctgga
      121 ccgtgaggtg acactgccgg agacaggtac atcgacggtg aacctgatta acggcagcgg
      181 taagccggtg cgcgtggaca tcactgcaca ccccgccccg gaccggatac aggtcagcgc
      241 cctgccggat ggcgtggaga catacggtgt gtggggactc tccctgccgt cactgcgtcg
      301 tcgcctgttc cgctgtgttt ccatccggga aaacacggac ggcacctttg ccatcacggc
      361 agtgcagcac gtaccggaaa aagaagccat cgtggataac ggggcccgct ttgagccgct
      421 ctcc
//
LOCUS       DAZUCE010000655          421 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZUCE010000655 DAZUCE010000000
VERSION     DAZUCE010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.658"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>421
                     /locus_tag="ACHY03_005496"
     CDS             <1..>421
                     /locus_tag="ACHY03_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFS3908563.1"
                     /translation="SAPCGLTNVGEQRGTYILKPLSMKGNLTAEKIENTGNIIECADL
                     QETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIELPGKSSYNLEEMLSAINF
                     TIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGN"
BASE COUNT          136 a           82 c           80 g          123 t
ORIGIN      
        1 agtgctccct gtggattgac caatgtcggg gaacaacgtg gaacatatat actcaagcca
       61 ctgagtatga aaggtaatct caccgccgaa aaaattgaaa acaccggaaa tatcattgag
      121 tgtgcagact tgcaagagac gactaacgcc attaaagttc attttttaac acagaacgct
      181 ttaaggttta aagtcggaag tcgtcattat attattaatt tcccctttga atctgacggt
      241 aaaaaaatag agcttccagg gaagtcatct tataatttag aagagatgct atctgcgata
      301 aatttcacca ttccatactc tatcgcatct gtatcagata gttccaacac cgcagatatt
      361 tttccaggaa ctccttttcc tttgttgttc gaaattgaag tgagttcatg tacagggaat
      421 a
//
LOCUS       DAZUCE010000656          420 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZUCE010000656 DAZUCE010000000
VERSION     DAZUCE010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.659"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            107..420
                     /locus_tag="ACHY03_005497"
                     /pseudo
     CDS             107..420
                     /locus_tag="ACHY03_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          142 a           76 c           94 g          108 t
ORIGIN      
        1 tcagaaacag aaaatgctga tgatgaattg tgaataatca aactgacctc taattatcct
       61 gtatggagta gtgcttattt tcgactcaat ttctactgat tagattatgg ttatcagtac
      121 agtgggttat gaaaaacgga ctggatctgc cagcagtgct tctattatta ttcaaaaaga
      181 acttcagccg gacaatgcta taatccaggg aaaggctata agctctgttg gacggaatgt
      241 ggaaatcagc actggtaaaa aggggaactg acattaacac aagaaggatt cctgcaagca
      301 actgcttatc tgcccggaac gcagaattaa agcatgacca cacaggagcc ggaatactgt
      361 ggtcgtacgc attcagtatt gtgacaacta atagaaataa aaatctggcc ggtgcgaata
//
LOCUS       DAZUCE010000657          418 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZUCE010000657 DAZUCE010000000
VERSION     DAZUCE010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.660"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>418)
                     /locus_tag="ACHY03_005498"
     CDS             complement(<1..>418)
                     /locus_tag="ACHY03_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3908564.1"
                     /translation="DSAVMYMNGRMEMYRYTPESLKRALVIFRDCVSTQPQNTLPYCC
                     LAECHISLALLGISEQKQAITTAVTSIEKALEINPSNSQALGLLGLISGLKDEHSVSN
                     VLFKQAHLLKPNSPDVYYYQSLLCFLNGDLARAFNL"
BASE COUNT          127 a           82 c           78 g          131 t
ORIGIN      
        1 attagattaa atgctcttgc aaggtcacca ttcaaaaaac atagcagaga ctgataataa
       61 taaacgtctg gcgaattagg tttgagtaaa tgtgcttgtt taaatagcac attagacact
      121 gaatgttcat cttttagccc actgattaaa cccagtaaac ccaatgcctg agaatttgaa
      181 gggttaattt ccagagcctt ttcaatggag gttactgccg tagttattgc ttgtttttgt
      241 tcactgattc caaggagtgc taatgatata tggcattccg ccaggcaaca gtagggcaag
      301 gtattttgcg gttgcgtact cacacaatct ctaaatatta ctaatgctcg tttcaaactt
      361 tctggtgtat aacggtacat ttccatcctg ccattcatat acataacagc agaatcca
//
LOCUS       DAZUCE010000658          409 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZUCE010000658 DAZUCE010000000
VERSION     DAZUCE010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.661"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            74..181
                     /locus_tag="ACHY03_005499"
     CDS             74..181
                     /locus_tag="ACHY03_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFS3908565.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(196..>409)
                     /locus_tag="ACHY03_005500"
     CDS             complement(196..>409)
                     /locus_tag="ACHY03_005500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908566.1"
                     /translation="SIHLHFIPEMFAMKSAFRFFNSCYSRFMSALDDASSSGCQYLLV
                     EVIKTKMAAGCYPCGRFRAHIAITAA"
BASE COUNT          114 a           98 c          109 g           88 t
ORIGIN      
        1 aacgggggct aatcttgaaa ccagacactt caagaatagc cgcaaaatgt tgtcattaca
       61 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcggc
      121 accgatcctg gcgggaatta ttaccgcagc gattgtcggc tggtggcgta accggaagta
      181 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
      241 gtaacagcct gcggccattt ttgtctttat cacttccaca agtaaatatt ggcaacccga
      301 gctggaagca tcatccagag cactcatgaa acgactgtaa cagctgttga aaaaccggaa
      361 tgcactcttc attgcaaaca tttcagggat gaagtgaaga tgaattgag
//
LOCUS       DAZUCE010000659          409 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZUCE010000659 DAZUCE010000000
VERSION     DAZUCE010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.662"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>409
                     /locus_tag="ACHY03_005501"
     CDS             <1..>409
                     /locus_tag="ACHY03_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFS3908567.1"
                     /translation="ALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLAGTTTLNNG
                     ATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVEKSGSGTLTVSNTTLIQKTVNLN
                     EGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGA"
BASE COUNT          107 a          123 c          114 g           65 t
ORIGIN      
        1 atgccctgat gctggaaaaa ggcagttcct tcacactgaa cgccggtgat acggccacgg
       61 ataccacggt aaatggcgga ctgttcaccg ccagaggggg ctcgctggcg ggcaccacca
      121 cgctgaataa cggtgccacg cttacccttt ccgggaaaac ggtgaataac gataccctga
      181 ccatccatga aggtgatgca ctcctgcagg gaggcgctct taccggtaac ggcagggtgg
      241 aaaaatcagg aagtggcaca ctcactgtca gcaacaccac actcatccag aaaaccgtca
      301 acctgaatga aggcacgctg acgctgaacg acagtaccgt caccacggat gtcatcgctc
      361 agcgcggcac ggccctgaag ctgaccggca gcacagtgct gaacggtgc
//
LOCUS       DAZUCE010000660          408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZUCE010000660 DAZUCE010000000
VERSION     DAZUCE010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.663"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(160..>408)
                     /locus_tag="ACHY03_005502"
     CDS             complement(160..>408)
                     /locus_tag="ACHY03_005502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908568.1"
                     /translation="QEVFKMREISDHMLDFVKGGMNLNGLPSSTNVIDLRGKDMGTYI
                     DANGACWAPGTPSIIMYPGGSGPSYSMSSSTSSANSGS"
BASE COUNT          121 a           90 c           76 g          121 t
ORIGIN      
        1 tatactgtag agttcacgta tgtaagtaat atatgaaaag gctctcgtta agatgtctgg
       61 tacaaaatga cagatcatat gcctttgtaa agagacgaca gaagtagagt cttacaggca
      121 catcataaat accctccggc catgccggag ggtatttatt taactgccgc tgtttgcact
      181 ggatgtggaa ctactcatac tataagaagg tccacttccc cccggataca tgatgatgga
      241 tggagtaccc ggagcccagc atgctccatt agcatcaata tatgttccca tatcttttcc
      301 tcgtagatct attacattag tagaactagg taatccatta agattcatcc ctcctttcac
      361 gaaatcaagc atgtgatctg atatttctct cattttaaaa acctcctg
//
LOCUS       DAZUCE010000661          408 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZUCE010000661 DAZUCE010000000
VERSION     DAZUCE010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.664"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>408
                     /locus_tag="ACHY03_005503"
     CDS             <1..>408
                     /locus_tag="ACHY03_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFS3908569.1"
                     /translation="SEVIQRLIQERISVRNMRLVMEALALWSPREKDIITLVEHVRGA
                     LGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEMILDNFKLA
                     LSGINIPIKDIILLGSVDIRRFIKKLIESSYRDL"
BASE COUNT          117 a           73 c           92 g          126 t
ORIGIN      
        1 ttcagaggta attcagcgtc tgatccagga gcgaatttca gtgcgcaata tgcggttagt
       61 tatggaggcc ttagcattat ggagcccacg agagaaagat ataatcacac tcgtcgagca
      121 tgtacgaggt gcattaggtc gctacatttg ccataagttt tcatattctg gagaaattaa
      181 agcaattgtg atttcgcctg aaatagagga cagaatcaga gatggtgtca ggcctaccgc
      241 tggcggtact tttcttaatc tcgatgcatc cgaagctgag atgattctgg ataattttaa
      301 actggcactt tctggaatca atattcctat taaagacatt attttactgg gatctgtgga
      361 tattcgtcgt tttatcaaaa aacttattga atctagttat cgagacct
//
LOCUS       DAZUCE010000662          404 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZUCE010000662 DAZUCE010000000
VERSION     DAZUCE010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.665"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
BASE COUNT          127 a           81 c           73 g          123 t
ORIGIN      
        1 agtgtcattt ggtgtagtcg caggtaccgg gtttattctt ttaatagccc ccattgttgc
       61 attactcctc aagtaatact catcaaacaa gaacgtgagg ggctctctcc ccaatctaat
      121 tatatatttg aggtaagtta caggaaaatg cgtaaagatg gataattgca cttgacctga
      181 cagcactgta aaaaaggact aaaactgtca aagctcaaga ctatccggcc ctgctctaaa
      241 tttttatagt ggtgaatctt gagctaatta accaacctgc taaaggctaa tgtaggtttt
      301 tgtccttgta tacctagatc acttccatca ggaatcctca gctttactgt gattaaaaaa
      361 taaccaaata attgctgcaa aacgtgattt acatcgcgat ttaa
//
LOCUS       DAZUCE010000663          402 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZUCE010000663 DAZUCE010000000
VERSION     DAZUCE010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.666"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..308
                     /locus_tag="ACHY03_005504"
     CDS             <1..308
                     /locus_tag="ACHY03_005504"
                     /inference="COORDINATES: protein motif:HMM:NF027273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFS3908570.1"
                     /translation="SKCAEIKIMTLKEFIKSLKTGEAKKFATNLGVSPSFLSQMASGT
                     SPVSPVRALMIEAETGGHVTRRELRPNDWELIWPEYTGITSPSRHKYELNTKKADGG"
     gene            312..>402
                     /locus_tag="ACHY03_005505"
     CDS             312..>402
                     /locus_tag="ACHY03_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFS3908571.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAEIAR"
BASE COUNT          129 a           84 c           91 g           98 t
ORIGIN      
        1 gttctaaatg tgctgagata aaaattatga cgctaaagga attcatcaaa tcattaaaaa
       61 ctggagaagc aaaaaaattt gcgaccaatc ttggtgtgtc tccgtctttc ttgtcccaaa
      121 tggcatctgg aacttcacct gtgtctcctg ttcgtgcgtt gatgatagaa gcagaaactg
      181 gaggacatgt tacaagacga gaattgagac ctaatgactg ggagcttata tggcccgaat
      241 acactggaat cacgtcccct tcacgtcaca aatatgaact taacaccaaa aaggctgatg
      301 gaggttgatt catgaaaatc aagcatgaac acatccgcat ggcgatgaat gcctgggcac
      361 atcctgatgg tgagaaagtt cccgcagctg aaatagcccg ga
//
LOCUS       DAZUCE010000664          388 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZUCE010000664 DAZUCE010000000
VERSION     DAZUCE010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.667"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>388
                     /locus_tag="ACHY03_005506"
     CDS             <1..>388
                     /locus_tag="ACHY03_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFS3908572.1"
                     /translation="VKLICKEAASETYRGALETLQKMMSECKYKEGNAFVIMGAGGQL
                     KRIKYDVDENKLKIFNIYFDNNYEVVTDGEPDVVCLSKQVWENLLIKLKLENKENTAS
                     EVHKSANKGEVEQLVECSKRNEKTLL"
BASE COUNT          147 a           48 c           84 g          109 t
ORIGIN      
        1 ttgtaaaatt aatttgtaag gaagctgcat cagagacgta tcgcggtgct cttgaaactt
       61 tacaaaaaat gatgtctgaa tgtaaatata aagaaggcaa cgcctttgtc attatgggag
      121 ctggagggca attaaaacgt attaaatatg atgttgatga aaataaatta aaaatattca
      181 acatatactt tgataataat tacgaggtag ttactgatgg tgagcctgac gtagtatgtt
      241 taagcaagca ggtctgggaa aatcttctca ttaaactaaa gctggagaac aaagaaaata
      301 cggcttctga agttcataaa tcagcgaata aaggtgaggt tgagcaatta gttgaatgct
      361 ctaagagaaa tgaaaaaacg cttcttga
//
LOCUS       DAZUCE010000665          385 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZUCE010000665 DAZUCE010000000
VERSION     DAZUCE010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.668"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            268..>385
                     /locus_tag="ACHY03_005507"
     CDS             268..>385
                     /locus_tag="ACHY03_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFS3908573.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDFWRRQIDKR"
BASE COUNT          109 a           78 c          111 g           87 t
ORIGIN      
        1 ccggcatcag gggcggcgga caaactgcaa ctgacagaca tatcaaaggc ggcgacatat
       61 acgttgctga ttgacagtgt gaaggtggaa gtgaacaacg cggatgccgc ggcatgataa
      121 aaaaggccgc cagcaatgaa ggaaagggat gaactttgtc cactacatga atgctggcgg
      181 ctggtaaaac gaccatgatg acaccgaagt ctgcggtgtt tttctacaaa aataagtaaa
      241 tcatgtcaac tatcacggtg agaaaccatg acatttctga accagttaat gctgtacttc
      301 tgtacggtgg tctgtgtgct gtatcttctt tcgggtgggt acagggccgt gcgcgatttc
      361 tggcgcaggc agattgataa aaggg
//
LOCUS       DAZUCE010000666          385 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZUCE010000666 DAZUCE010000000
VERSION     DAZUCE010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.669"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..305)
                     /locus_tag="ACHY03_005508"
     CDS             complement(<1..305)
                     /locus_tag="ACHY03_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFS3908574.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT          104 a           79 c           89 g          113 t
ORIGIN      
        1 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
       61 ttattgatcg acaaatccat caatttatgg acgttttcgt cgtcccaggt ctggcagaaa
      121 gtggccaggt tctgacgagc gttgccatca agatataatt catcattgat aatctggaat
      181 gcgacatcat cgcgcatttc gtgcagcgga aatcgttttg actccgcgat agtagaaatg
      241 gcctttgcgc caaaacgtga atcgagtagt tctgagcgga aatccgttaa cagcttctgg
      301 tccatttcga actccttaaa tttatttgaa ggcaataaaa aagtaggatt tatccgcaat
      361 ggaagcaagg cattacaggc ttaat
//
LOCUS       DAZUCE010000667          381 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZUCE010000667 DAZUCE010000000
VERSION     DAZUCE010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.670"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>381
                     /locus_tag="ACHY03_005509"
     CDS             <1..>381
                     /locus_tag="ACHY03_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908575.1"
                     /translation="GGTRMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQDAVN
                     VSQLNREISQATAATTWALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGNYSY
                     EIDVTGIPVEYTDSQGNPLVNIGGR"
BASE COUNT           98 a           87 c          120 g           76 t
ORIGIN      
        1 cggcggaacc cggatggata aaaacgggct gaccatcaag gatggcccgt cagtgactgt
       61 gaacggtatt gatgcggctg gccgggttat ttccggcgtg aaagacgggg tgaatgacca
      121 ggacgccgtt aacgtttcac aactgaaccg tgaaatcagt caggcaacgg cagcaacgac
      181 ctgggcactg aagactgaat ccggtgatga ggctgttgct gtttcttccc agacagttga
      241 agtccgccac ggacagaata cccgggtttc agccatcagt aaggatgaca agggaaatta
      301 cagctatgag attgatgtga cggggattcc ggtggagtac accgacagcc aggggaaccc
      361 gcttgtcaat attggtggtc g
//
LOCUS       DAZUCE010000668          378 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZUCE010000668 DAZUCE010000000
VERSION     DAZUCE010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.671"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..370
                     /locus_tag="ACHY03_005510"
     CDS             <1..370
                     /locus_tag="ACHY03_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908576.1"
                     /translation="YTNIDDFKNLLKDLINTMTNGKVEFNSEQLSKLMAEITSFESNL
                     DGTPKDINFISQNEHLRQTVSIRQDGLCTKITLIVEETHFDIDHGDILHGKKWSETRV
                     LPEELNMAIVVENLRNLPHS"
BASE COUNT          146 a           69 c           60 g          103 t
ORIGIN      
        1 ttatacaaat attgatgatt ttaaaaacct cttaaaggac cttattaata ccatgacgaa
       61 tggaaaagtc gagtttaatt ccgagcaatt atctaaactc atggcagaaa taacctcttt
      121 cgagtccaat cttgatggaa cccccaaaga tataaatttt atctctcaaa atgaacatct
      181 acgccaaact gtatccatca gacaagatgg gctatgcacg aaaataacac tcattgtaga
      241 agaaacccat tttgatatag accatggtga tatcttacat ggaaaaaaat ggagtgaaac
      301 aagagtgctg cctgaagaat taaacatggc tattgttgtt gaaaacctta gaaacttacc
      361 acacagttaa acaaagtt
//
LOCUS       DAZUCE010000669          377 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZUCE010000669 DAZUCE010000000
VERSION     DAZUCE010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.672"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>377)
                     /locus_tag="ACHY03_005511"
     CDS             complement(<1..>377)
                     /locus_tag="ACHY03_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HFS3908577.1"
                     /translation="HFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPEQHQS
                     LTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTVSAFI
                     QFLALITPLFFQVVMDKVLVHRG"
BASE COUNT          139 a           79 c           62 g           97 t
ORIGIN      
        1 cccccggtga actaaaacct tatccattac aacctgaaaa aaaagaggtg ttattaacgc
       61 aagaaactga ataaaagcag aaacagttaa cacctcaagt aaaatcctcc tgtatttcac
      121 aacagagggg ataaaccagg aaaaatcaaa tttagctaac tctccgacta ctgttgctct
      181 tgacgtaacc agaatgactt ttccctgata cagtttttcg aactcatccc gactaaaagt
      241 tagtgactga tgctgttctg gatcataaac aagatagcgt gatgaatctt tagtaacacg
      301 agataatata taatgcttac cgtcatcccg ccatatcaac gcaggtaaag aaattattga
      361 caatctggaa aaatgtt
//
LOCUS       DAZUCE010000670          377 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZUCE010000670 DAZUCE010000000
VERSION     DAZUCE010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.673"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>377)
                     /locus_tag="ACHY03_005512"
     rRNA            complement(<1..>377)
                     /locus_tag="ACHY03_005512"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           78 a          128 c           75 g           96 t
ORIGIN      
        1 cttttcctgg aagcagggca tttgttgctt cagcaccgta gtgcctcgtc atcacgcctc
       61 agccttgatt ttccggattt gcctggaaaa ccagcctaca cgcttaaacc gggacaaccg
      121 tcgcccggcc aacatagcct tctccgtccc cccttcgcag taacaccaag tacaggaata
      181 ttaacctgtt tcccatcgac tacgcctttc ggcctcgcct taggggtcga ctcaccctgc
      241 cccgattaac gttggacagg aacccttggt cttccggcga gcgggctttt cacccgcttt
      301 atcgttactt atgtcagcat tcgcacttct gatacctcca gcatacctca cagtacacct
      361 tcacaggctt acagaac
//
LOCUS       DAZUCE010000671          374 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZUCE010000671 DAZUCE010000000
VERSION     DAZUCE010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.674"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
BASE COUNT          129 a           82 c           78 g           85 t
ORIGIN      
        1 taacggcatc acgaatgagt ggaggcagcg agctcagggg aaactcatca ggatactgaa
       61 agtatggcat aatgacctca caacacacag aacataaagt accgcaacta ttgctactaa
      121 tagcaaaaat agcagcagaa tcaatacagg ataaaaattt ctgtcgattt tcaggtcaaa
      181 taataaccac tcgatatttt gtcgctaaag aatgtcagaa tgaatctgtg cgtctacggg
      241 cgctctccgg agcacacatg ttgattaatt ttcacacaac aacgtaccag caaaaagcat
      301 catccagggt attgcaggta cgggtttcct gccgggaata aacataaaca gtttccccgg
      361 agtaacgctt tcag
//
LOCUS       DAZUCE010000672          372 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZUCE010000672 DAZUCE010000000
VERSION     DAZUCE010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.675"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
BASE COUNT          128 a           61 c           93 g           90 t
ORIGIN      
        1 gacgggaaag tggtggttat cggacgggga taaaaataaa aaaatcccgc agagttagcg
       61 gagctgcggg aagagatacg aagattaact gtaaggagtt attattatgt cacagcaaaa
      121 aaaaacggta acgcagagaa attataagcg ccactgggat tttgtgaaaa tgtgaagaat
      181 ttcagaaatt ttctgttgcg gagctgaact aaaaaacgct cccgcaatac acctggatat
      241 taacgggagg aacgaatagt caccttaagg agttgcgatt aattgctttt attgaccgca
      301 ggtaatctac ccatccggca aggtttcctc cacgggaatt tgccggaaat gtgaagaatt
      361 tcagaaattt ta
//
LOCUS       DAZUCE010000673          359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZUCE010000673 DAZUCE010000000
VERSION     DAZUCE010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.676"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>359)
                     /locus_tag="ACHY03_005513"
     rRNA            complement(<1..>359)
                     /locus_tag="ACHY03_005513"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           80 a          104 c           86 g           89 t
ORIGIN      
        1 aaacattaaa gggtggtatt tcaaggtcgg ctccatgcag actggcgtcc acacttctaa
       61 gcctcccacc tatcctacac atcaaggctc aatgttcagt gtcaagctat agtaaaggtt
      121 cacggggtct ttccgtcttg ccgcgggtac actgcatctt cacagcgagt tcaatttcac
      181 tgagtctcgg gtggagacag cctggccatc attacgccat tcgtgcaggt cggaacttac
      241 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt taccggggct
      301 tcgatcaaga gcttcgcttg cgctgacccc atcaattaac cttccggcac cgggcaggc
//
LOCUS       DAZUCE010000674          359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZUCE010000674 DAZUCE010000000
VERSION     DAZUCE010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.677"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..353
                     /locus_tag="ACHY03_005514"
     CDS             <1..353
                     /locus_tag="ACHY03_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFS3908578.1"
                     /translation="HFYRERFAERGFFYKVPAVLRDYLSAIPVEINEKARYKPGIANY
                     HNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHIQLPLPLASTLLERITFYAKKN
                     RDELDKISCKWCCD"
BASE COUNT          117 a           64 c           66 g          112 t
ORIGIN      
        1 gtcactttta tcgagaaagg tttgcagaac gcggattttt ttataaggtt cctgctgtgc
       61 taagggatta tttatcagct ataccggtgg aaataaatga aaaggcacga tacaaacccg
      121 gcatcgctaa ctatcacaat atcatcacat gtggtttttc aacgttgtta ccctatataa
      181 ggcaacaacc attggcaatg cagcaacgat ttaatttatt atttcctgat tttgttgacc
      241 acatacaatt gccgttacca cttgcgtcaa ctcttttaga gagaattaca ttttatgcga
      301 aaaaaaatag agatgagctt gataaaatct cctgcaaatg gtgttgtgat taaaagaaa
//
LOCUS       DAZUCE010000675          359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZUCE010000675 DAZUCE010000000
VERSION     DAZUCE010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.678"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(65..358)
                     /locus_tag="ACHY03_005515"
     CDS             complement(65..358)
                     /locus_tag="ACHY03_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFS3908579.1"
                     /translation="MSLVGYNSKIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFALVTTVPAYKEMSMYQLLLISQDRLTH"
BASE COUNT          106 a           63 c           62 g          128 t
ORIGIN      
        1 taatttttat cgtagtcaga tatttaaaaa taatctacac gcaaaaagcg tgtagattca
       61 ttatttaatg agttaaacga tcttgcgaaa tgagaagcaa ttgatacata ctcatctctt
      121 tataagctgg aactgtagta accagtgcaa attcgcgata tagcttttgc tccagttctg
      181 ctgaagatat atattccggg tgttcgctaa agaactgtct tgcatctact atcgcttggg
      241 attcgccact tcttttattt tgattctcct ttaatgaatt ttctctggtg catgcagaga
      301 gcaacgatat cagcgcgatg gttattatga ttttactatt atatcctact aatgacata
//
LOCUS       DAZUCE010000676          359 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZUCE010000676 DAZUCE010000000
VERSION     DAZUCE010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.679"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(191..>359)
                     /locus_tag="ACHY03_005516"
     CDS             complement(191..>359)
                     /locus_tag="ACHY03_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS3908580.1"
                     /translation="GRYDTLAHFPDKPAVWLERDDETGFQQVLERSGYLAKEHTKARG
                     TGERNFGCSRS"
BASE COUNT           79 a           94 c           98 g           88 t
ORIGIN      
        1 tgtgaatggc ggttcagtta aaatctggac tggagtttgg gtaaagcagg ctggttgctg
       61 ttacagcact gtggttgtca cacaatggcc cactgacgta aaaaacagtc agtgggcgag
      121 tggatacatc gttgccgcca gacaacaacc catacttttg gtttgccggg tattggtgcg
      181 cctgtcgtgt tcaactccgg ctacagccaa aattgcgctc accggtgccg cgagctttgg
      241 tatgctcctt cgccagatag ccagaacgtt ccagcacctg ctgaaagccg gtttcatcat
      301 cgcgttccag ccacaccgct ggcttgtcag gaaaatgtgc cagcgtgtca taacgtccg
//
LOCUS       DAZUCE010000677          356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZUCE010000677 DAZUCE010000000
VERSION     DAZUCE010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.680"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
BASE COUNT           98 a           64 c           65 g          129 t
ORIGIN      
        1 gtctacttac catatttgtc gttattgctg ctgtacgagc ccttattacg tcaaacaccc
       61 gactacgttt aaggttttac ctagcgtagt tgaattgggt aattattgtg tttgtgttct
      121 tactaagcaa ttatttggtg tttgcactag ctttaaaatc gctagactta tattttagct
      181 ttggcccctg aaaatggaga ttagatagtt caagtgctgt atacctgata tcactcgcag
      241 ttgctattta ccttttattt cgcttaaaaa actaaaatct caattggaag aacaggaatc
      301 tttctagtgt tagttgaaaa tgtgcacctt actaaacgat acgatgatct ggcaca
//
LOCUS       DAZUCE010000678          356 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZUCE010000678 DAZUCE010000000
VERSION     DAZUCE010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.681"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(197..>356)
                     /locus_tag="ACHY03_005517"
     CDS             complement(197..>356)
                     /locus_tag="ACHY03_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908581.1"
                     /translation="QCSFTLTWGARVGRLAFSRLYPCYTELNKIRAMQLNNAYISDFQ
                     NPDEISSK"
BASE COUNT          113 a           56 c           71 g          116 t
ORIGIN      
        1 tcagtttcga tatcgaatgc taaatctaca aagcaataat ctacgtagaa cataaatcta
       61 cgtatatctt ccggtatagc agtggaaaaa taacggtatt gcgtgtaagt ggtttgatca
      121 tcgaattcgt ctgattgtac tgaatatgta aatgtggcca gtataaatag taatgttaat
      181 cttaaattta taataatcat ttgctggata tctcatctgg attttgaaag tcagaaatgt
      241 aagcattgtt tagttgcatg gctcttattt tgtttaactc agtatagcat ggatatagcc
      301 gagagaatgc cagtcgccct actctggccc cccaggttag agtaaaagag cattga
//
LOCUS       DAZUCE010000679          352 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZUCE010000679 DAZUCE010000000
VERSION     DAZUCE010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.682"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..225)
                     /locus_tag="ACHY03_005518"
     CDS             complement(<1..225)
                     /locus_tag="ACHY03_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFS3908582.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVR"
BASE COUNT          121 a           78 c           75 g           78 t
ORIGIN      
        1 gcggacaaaa atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat
       61 gctgatcatc agtgccaaag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc
      121 gaaagtatta atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc
      181 ccaccattta tcacgtattt ttatataagt tgcattcagt ttcatagatg ctcagcagaa
      241 tcccccacat cctgaaggag gtgtattcag acaggcatcc cacctgactt cgaatgatga
      301 ttattcatca ctatagagag cattgattct aagtgtcata tgaaagttcc aa
//
LOCUS       DAZUCE010000680          347 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZUCE010000680 DAZUCE010000000
VERSION     DAZUCE010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.683"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            133..>347
                     /locus_tag="ACHY03_005519"
     CDS             133..>347
                     /locus_tag="ACHY03_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFS3908583.1"
                     /translation="MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDA
                     IYRSHDTIGPYYVVGPGIAMPHARPEEG"
BASE COUNT           95 a           75 c           87 g           90 t
ORIGIN      
        1 agagaaatga tagcgctatc acaaagatcg gtagcgttat cagatagcgc tatcaaatgc
       61 gacaatcatc acattaatta atgcgtaaaa tatctactct cctgttaatt atcatcggga
      121 gttatcagtc gcatgttaaa aaatctatta aatacagagg tcgttcaggt cgtggaacag
      181 gccaaagact ggcgggaagc cgtggcgatt tcatgccggc cgttgattga aaacggttct
      241 atcgaaccgc gttatgttga tgcgatctat cgctcgcacg acactatcgg tccgtactat
      301 gtggttggcc ctggtattgc gatgcctcat gcgcgcccgg aagaggg
//
LOCUS       DAZUCE010000681          343 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZUCE010000681 DAZUCE010000000
VERSION     DAZUCE010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.684"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..330
                     /locus_tag="ACHY03_005520"
     CDS             <1..330
                     /locus_tag="ACHY03_005520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908584.1"
                     /translation="TKNRQNQAVICCRFGSRPLQQIDLCCMSNAGASQQSNIRQQLSF
                     CQAKESNYCLFSVLSTPALSAVSQTGAGFRKGCSVAVNAGVSQRLLSRQYRQWCCRDA
                     VFAWTAQ"
BASE COUNT           78 a           83 c           90 g           92 t
ORIGIN      
        1 acaaaaaacc gccaaaatca ggcggttatt tgttgccggt ttggttcgcg gcctctccag
       61 cagattgact tgtgttgcat gagcaacgca ggtgcttcac agcagagcaa tattcgccag
      121 caactctctt tttgtcaagc aaaagagagt aattattgtt tatttagcgt attatcgaca
      181 ccggcccttt ccgccgtctc gcaaacgggc gctggcttta ggaaaggatg ttccgtggcc
      241 gtaaatgcag gtgtttcaca gcgcttgcta tcgcggcaat atcgccagtg gtgctgtcgt
      301 gatgcggtct tcgcatggac cgcacaatga agatacggtg ctt
//
LOCUS       DAZUCE010000682          341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZUCE010000682 DAZUCE010000000
VERSION     DAZUCE010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.685"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>341
                     /locus_tag="ACHY03_005521"
     CDS             <1..>341
                     /locus_tag="ACHY03_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFS3908585.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKIL"
BASE COUNT           76 a           87 c          105 g           73 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg a
//
LOCUS       DAZUCE010000683          341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZUCE010000683 DAZUCE010000000
VERSION     DAZUCE010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.686"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            88..309
                     /locus_tag="ACHY03_005522"
     CDS             88..309
                     /locus_tag="ACHY03_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908586.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT           81 a           76 c           94 g           90 t
ORIGIN      
        1 tataacctcc ggtgataaaa tggcattcag ctcgttaata agagagttaa cttattaagc
       61 gttagcgttt attactgagg taacaccatg aaaagattaa ttcctgtggc attgctcacc
      121 gcattgctgg cgggctgtgc tcacgattcg ccctgcgttc cggtttatga cgatcagggg
      181 cgtctggttc acaccaatac ctgtatgaaa ggcacaactc aggataactg ggaaacggct
      241 ggggctattg ctggcggagc agcagctgtt gctggcctga cgatggggat catcgctctg
      301 tcgaaataac tgaacagggc gcggagtttc gcgctctgtt t
//
LOCUS       DAZUCE010000684          341 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZUCE010000684 DAZUCE010000000
VERSION     DAZUCE010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.687"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>341)
                     /locus_tag="ACHY03_005523"
     CDS             complement(<1..>341)
                     /locus_tag="ACHY03_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFS3908587.1"
                     /translation="DFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVG
                     FRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMDAFGCTSRG
                     QAHRAGLWVIK"
BASE COUNT           65 a          119 c           91 g           66 t
ORIGIN      
        1 tctttatcac ccacagtccg gcacggtggg cctgaccgcg gctggtacag ccaaacgcat
       61 ccatcttcag cagattgcgc ccgtagcgca ggatggcgtc cgggtcttcc accagttccg
      121 tggaagtctg ccagccgttc tgcgggtcgg tgtaattcac ctccaccgcc gtgtgccggt
      181 ccttcagggc actgaagctg tagcggaacc ccacgccgtt atcatccacc accacatcgc
      241 tgttggtgta cggccacacc acatccgacg gacggtcctg aacgaacgtc agcgtctggc
      301 cgttccatac cggcatacag cgcatcgccg agcagaaatc c
//
LOCUS       DAZUCE010000685          340 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZUCE010000685 DAZUCE010000000
VERSION     DAZUCE010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.688"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>340)
                     /locus_tag="ACHY03_005524"
     CDS             complement(<1..>340)
                     /locus_tag="ACHY03_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFS3908588.1"
                     /translation="FPCVNISGEPEAYFRMAPEDWLRAEMQGEIVALVHSHPGALPWL
                     SEADRRLQVQSDLPWWLVCRGAIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIE
                     MPDFHREDDWW"
BASE COUNT           80 a          124 c           85 g           51 t
ORIGIN      
        1 gccaccagtc atcctcacga tgaaaatcag gcatctcaat tcccgccaga tggtaagcat
       61 cccggaacag cgtgtaacag tccgtcaccc cgtgctcaaa gcgccgcccg gtaagatgtg
      121 gcacacagcg gaacttgtga atcgcccccc ggcagaccag ccaccacggc aaatcactct
      181 gcacctgcag ccgccggtcg gcctcactca gccagggcag agcaccgggg tgactgtgga
      241 ccagcgccac aatctcaccc tgcatttctg cccgcagcca gtcctccgga gccatccgga
      301 aatacgcctc cggctcaccg gagatattca cgcagggaaa
//
LOCUS       DAZUCE010000686          340 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZUCE010000686 DAZUCE010000000
VERSION     DAZUCE010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.689"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>340)
                     /locus_tag="ACHY03_005525"
     CDS             complement(<1..>340)
                     /locus_tag="ACHY03_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFS3908589.1"
                     /translation="YMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFIN
                     VANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYGE
                     QWMNMAQPFWA"
BASE COUNT           88 a           90 c           93 g           69 t
ORIGIN      
        1 gcccagaatg gttgtgccat gttcatccat tgctcgccgt aggcgatcgc cattaccgat
       61 ttaccgagat cagcgcccag cgcctgggct gcgggtatca cgaaaggtcc ctgaataacc
      121 cagtgaccgc cgccagacgg aacggcgaag ttaatcagtg cagaactaaa aaaggtcatt
      181 accgggaagg tgtctttgtt cgcaacattg atgaagaatt cggtaatgag tccgcccaga
      241 ccggaatgct ccatcatcag ttggatccca gcgtagaagg ggaattgcac cagaataccg
      301 gcagtactgc gtgctgccgc gctgatagca cgcatataag
//
LOCUS       DAZUCE010000687          339 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZUCE010000687 DAZUCE010000000
VERSION     DAZUCE010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.690"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>339)
                     /locus_tag="ACHY03_005526"
     CDS             complement(<1..>339)
                     /locus_tag="ACHY03_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III effector protein"
                     /protein_id="HFS3908590.1"
                     /translation="LVGFDKVTCESYDVLCNVKPNNKIPIYGMFKGCFLYQCEMKNFK
                     IETSKIYSINQDPQRGDKKVGAYLFMQSFVYASNLQDGVCKEASVIASSLLSCNIAKL
                     NGVGMDFIETS"
BASE COUNT          107 a           70 c           43 g          119 t
ORIGIN      
        1 cgaagtttct atgaaatcca ttccaacacc attaagcttt gctatattgc aagataataa
       61 actgcttgca ataacacttg cttctttaca cactccatct tgaagattac ttgcatatac
      121 aaatgactgc ataaacagat atgcaccgac tttcttatcc cctctttggg gatcttgatt
      181 tatagagtat atttttgatg tctctatctt gaaatttttc atttcgcatt gatacaaaaa
      241 acacccttta aacataccgt aaatcggtat tttattattt ggttttacgt tacaaagaac
      301 gtcatagctt tcacaggtca ctttatcaaa gccaacaag
//
LOCUS       DAZUCE010000688          339 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZUCE010000688 DAZUCE010000000
VERSION     DAZUCE010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.691"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..279)
                     /locus_tag="ACHY03_005527"
     CDS             complement(<1..279)
                     /locus_tag="ACHY03_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFS3908591.1"
                     /translation="MKKASKLLLALIPALSLSVFSAGVYAADASGINGTSYYGVTVDQ
                     LKGHEYKNLIDFWKDLGFSGKAYLDGKVVNAGTKQPDSVNIQNTAGKYL"
BASE COUNT           95 a           72 c           53 g          119 t
ORIGIN      
        1 taaatatttc cctgccgtat tttgtatatt tacactatct ggttgttttg tgcctgcatt
       61 taccactttt ccatccagat atgcttttcc tgagaagccg aggtctttcc aaaaatcaat
      121 taaattttta tattcatgtc ctttcaactg atcaacagtc actccataat atgaagtgcc
      181 attaattcca gaggcgtctg ctgcatatac gccagcagaa aataccgaca ggcttaatgc
      241 tggtattaaa gccaacaata gcttgcttgc ctttttcatg atgtgtaccc ttatgttaaa
      301 tcatgttata aatatgttga cattcggaac gcaaatgtc
//
LOCUS       DAZUCE010000689          338 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZUCE010000689 DAZUCE010000000
VERSION     DAZUCE010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.692"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>338)
                     /locus_tag="ACHY03_005528"
     CDS             complement(<1..>338)
                     /locus_tag="ACHY03_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFS3908592.1"
                     /translation="LIFDFIAEYFLFMKDMKMDKQEVKREYKEQEGNPEIKSKRRERH
                     QEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAK
                     EIGIPIITDK"
BASE COUNT           98 a           62 c           50 g          128 t
ORIGIN      
        1 tttatctgta ataattggta tcccgatttc ctttgcatat ttccttacgg ctaatgctac
       61 ctcattagtt tctcttacag aaatcaatgg aataggtgaa agatgtggct taaagtatat
      121 ccctattgca atgtgagtag ggttggcaat catcaaacga ctattactga catcagactt
      181 caattgctca gaaagaattt cctgatggcg ctctctgcgt ttagacttaa tttctggatt
      241 tccttcttgt tccttgtatt ctcttttaac ttcttgttta tccattttca tatctttcat
      301 aaataaaaaa tattcagcga taaaatcaaa aattaaga
//
LOCUS       DAZUCE010000690          335 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZUCE010000690 DAZUCE010000000
VERSION     DAZUCE010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.693"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>335)
                     /gene="tssK"
                     /locus_tag="ACHY03_005529"
     CDS             complement(<1..>335)
                     /gene="tssK"
                     /locus_tag="ACHY03_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFS3908593.1"
                     /translation="RDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSMRRENN
                     ARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLLTFSLE
                     HNVDAVPAYR"
BASE COUNT           87 a           99 c           96 g           53 t
ORIGIN      
        1 cggtatgccg gaaccgcatc cacattatgc tcaagcgaga atgtgagcag ggagcctgcc
       61 agccgggcaa gctccctgta caacagttcc gggtggcggt aaggcatgtc aagcaactct
      121 ttcagaacag gctccgcact gttcagggca ttcagcaacc agaacaacga cacgtccgcc
      181 actgcaaaat ccgcaagacg ggcattgttc tcccgacgca tggacatcag tcgctggcga
      241 cgcgcctgca ggtgatgaag caactccgca agctcagtca tcagcgaagg actggcagac
      301 agcgtcagca gagggggaat aaaacgcggg tccct
//
LOCUS       DAZUCE010000691          334 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZUCE010000691 DAZUCE010000000
VERSION     DAZUCE010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.694"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>334
                     /locus_tag="ACHY03_005530"
     CDS             <1..>334
                     /locus_tag="ACHY03_005530"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3908594.1"
                     /translation="TNFRSITKAHKVSISDTTILIGRNNEGKSNLLRALDVAMSSLQR
                     HALEYRYGKLGVRSISRRDEKTFYWERDFPINLQSRKSGTQTIIRLEFLLNDDEVTEF
                     KNEIKSNVN"
BASE COUNT          125 a           49 c           63 g           97 t
ORIGIN      
        1 aacaaatttt agaagtataa cgaaagcgca taaagtatca atatctgaca ctactatatt
       61 aattggtaga aataatgaag gcaagtctaa tttattaaga gcacttgatg ttgcaatgag
      121 ttctctgcaa cgccatgcct tagaatatcg ctatggaaaa cttggtgtaa ggagtatatc
      181 tcgcagagat gagaaaacct tttattggga acgtgacttc cctattaact tacaatctag
      241 aaaatccgga actcaaacaa ttattaggtt agagtttctg ttaaatgatg atgaggtaac
      301 tgagtttaag aacgaaatca agtcaaatgt aaac
//
LOCUS       DAZUCE010000692          333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZUCE010000692 DAZUCE010000000
VERSION     DAZUCE010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.695"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..309
                     /gene="hcp"
                     /locus_tag="ACHY03_005531"
     CDS             <1..309
                     /gene="hcp"
                     /locus_tag="ACHY03_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFS3908595.1"
                     /translation="TKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYFNTK
                     LENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNERTTA
                     "
BASE COUNT           96 a           80 c           88 g           69 t
ORIGIN      
        1 accaaggaaa ttgattcctc cagcccctac ctttacaagg cggtgaccac ggggcagacc
       61 ctgaagtctg ctgaatttaa gtggtacaga atcaatgatg ccggtcagga agtggagtat
      121 ttcaatacga agcttgagaa cgtgaaggtg gtgaaggtta acccggtcat gcacgacatc
      181 aagaatcccg cctacgagaa gcataaccac cttgagcagg tggagctgcg ctacgagaaa
      241 atcacctgga cttataagga cggtaacatc attcattctg acgcgtggaa tgaacgtacc
      301 actgcgtaat tctcccggac gggcagggta tcc
//
LOCUS       DAZUCE010000693          333 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZUCE010000693 DAZUCE010000000
VERSION     DAZUCE010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.696"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>333
                     /locus_tag="ACHY03_005532"
     rRNA            <1..>333
                     /locus_tag="ACHY03_005532"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           84 a           76 c          110 g           63 t
ORIGIN      
        1 gagtaatgtc tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata
       61 ccgcataacg tcgcaagacc aaagaggggg accttcgggc ctcttgccat cggatgtgcc
      121 cagatgggat tagctagtag gtggggtaac ggctcaccta ggcgacgatc cctagctggt
      181 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
      241 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      301 aggccttcgg gttgtaaagt actttcagcg ggg
//
LOCUS       DAZUCE010000694          331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZUCE010000694 DAZUCE010000000
VERSION     DAZUCE010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.697"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            24..100
                     /locus_tag="ACHY03_005533"
     tRNA            24..100
                     /locus_tag="ACHY03_005533"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="ACHY03_005534"
     tRNA            143..218
                     /locus_tag="ACHY03_005534"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           96 a           71 c           81 g           83 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg caaaggttca ctgcgaagtt ttgctcttta aaaatctgga
      301 tcaagctgaa aattgaaaca ctgaacaacg a
//
LOCUS       DAZUCE010000695          331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZUCE010000695 DAZUCE010000000
VERSION     DAZUCE010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.698"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>331
                     /locus_tag="ACHY03_005535"
     rRNA            <1..>331
                     /locus_tag="ACHY03_005535"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           85 a           75 c          111 g           60 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga c
//
LOCUS       DAZUCE010000696          331 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZUCE010000696 DAZUCE010000000
VERSION     DAZUCE010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.699"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            112..247
                     /locus_tag="ACHY03_005536"
                     /pseudo
     CDS             112..247
                     /locus_tag="ACHY03_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_307391.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          117 a           49 c           60 g          105 t
ORIGIN      
        1 ataggtggtt tagccataat cgtctcaaac caacacccag gtatgctttt aagacaaatc
       61 ggctatgtgt tgtgcttggg cgaataaata ttgtgttcag gagaaatgat tatggctata
      121 tcatcatata gtacagttac agataaattt gctaatctga ctaaaatgcc agatgaaaat
      181 aatggattgc aaaaaaaatc agaaaaattt tgtttgtctg ttggtaatga ggagaagtat
      241 tcaataaccc ataaagatat aacaaacctt atagctggtg atagacaatc ttttcttaaa
      301 ctaactcttt gggaatttat actcgatctc t
//
LOCUS       DAZUCE010000697          328 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZUCE010000697 DAZUCE010000000
VERSION     DAZUCE010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.701"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>328
                     /locus_tag="ACHY03_005537"
     CDS             <1..>328
                     /locus_tag="ACHY03_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908596.1"
                     /translation="QITDQQLQITYTSVKTDNYKQIYDEISAVDEGLSPYDVLRYQRA
                     IKTLIINEGEKGHLDTLLVSPSDLDRLEESVKQGKNLVVALGDKKYVFTQVKEINYLE
                     DYLFDKN"
BASE COUNT          126 a           48 c           57 g           97 t
ORIGIN      
        1 cagattaccg atcaacagct acaaattaca tatacatctg ttaaaacaga taactataaa
       61 cagatctatg atgagatcag tgctgttgat gaagggctct caccttatga tgttttaagg
      121 tatcaaagag ccattaaaac acttatcatt aatgagggtg aaaaagggca cttagatact
      181 ctattggttt caccttctga cttagacaga cttgaagaaa gtgttaaaca aggaaaaaat
      241 ttagttgttg ctctaggcga taaaaagtat gtattcacac aagttaaaga aataaattat
      301 cttgaagact acttatttga taaaaatg
//
LOCUS       DAZUCE010000698          328 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZUCE010000698 DAZUCE010000000
VERSION     DAZUCE010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.702"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(31..>328)
                     /locus_tag="ACHY03_005538"
     CDS             complement(31..>328)
                     /locus_tag="ACHY03_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908597.1"
                     /translation="ALIHGAGLDKDASVAIGTAAGGFLGKATKIPSADIAGSAIGGYI
                     GGVIADGSNRTITIPSNGINYNPSIGLGSFNPNYNGGLLNSGFNSSTSSSSSGS"
BASE COUNT           99 a           72 c           48 g          109 t
ORIGIN      
        1 cataataagc tatgctgctt tagaaattaa tcaacttcca ctactagaag aggatgttga
       61 tgagttaaat cctgagttta ataaacctcc attgtagttt ggattaaaac ttccaagtcc
      121 tatactcgga ttatagttaa taccgtttga tggtattgtt attgtcctat ttgaaccatc
      181 agcaataact cctccaatat atcctccaat tgcacttcct gctatatccg cactagggat
      241 ttttgttgct ttacctaaaa aaccaccagc cgcagtacct attgcaacgg atgcatcttt
      301 atctaatcct gcaccatgta ttaaagca
//
LOCUS       DAZUCE010000699          326 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZUCE010000699 DAZUCE010000000
VERSION     DAZUCE010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.703"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>326
                     /locus_tag="ACHY03_005539"
     CDS             <1..>326
                     /locus_tag="ACHY03_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908598.1"
                     /translation="VFNVVGENGQVTEAVLTQDGPVLENFTAGQSGTLAEGINNGIIW
                     GGIAFGAGLAAIALAAGGGGGSSRHKRATEGEQKPVQPEGGQEAGKTDVLSTGSTEAG
                     SGSSSD"
BASE COUNT           77 a           62 c          114 g           73 t
ORIGIN      
        1 gtgtttaatg ttgtgggtga aaatggtcag gttactgaag ctgtcctgac ccaggatggt
       61 cctgtactgg agaatttcac tgcagggcaa agtggcacac tggctgaagg tatcaataac
      121 gggattatct ggggggggat tgcttttggt gcagggctgg cggctattgc actggctgcc
      181 ggaggcggtg gtggaagcag tcgtcataag cgtgcaactg aaggtgagca gaagccggtt
      241 cagcctgaag gcggtcagga ggctggaaaa acagatgtat tatccacagg ctcaactgag
      301 gcgggaagtg gaagctcttc cgacca
//
LOCUS       DAZUCE010000700          325 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZUCE010000700 DAZUCE010000000
VERSION     DAZUCE010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.704"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>325
                     /locus_tag="ACHY03_005540"
     CDS             <1..>325
                     /locus_tag="ACHY03_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFS3908599.1"
                     /translation="GMSYGEGLPLPETYDRPDPRIKQLARRSTVTPGGAACKYNDIIP
                     ADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFIPANAGILLVPCCRGA
                     SAFTTG"
BASE COUNT           67 a           89 c          103 g           66 t
ORIGIN      
        1 acggcatgtc gtatggtgaa ggccttccgc tgccggagac atatgaccgt ccggacccgc
       61 gtattaagca gctggcgcgc cgcagtacgg tgacaccggg cggtgccgcc tgtaagtata
      121 acgacattat tccggcggac cattgtctgc atgatgtgca ggacatgagc cgtcttaacc
      181 atccgaaagc ggacctgtca aaggggcagt acggaaccgt ggggcagggg ctgcatatcg
      241 ccaaaaaact gctgccgttt ataccggcga atgcgggcat tctgctggtt ccgtgctgcc
      301 gtggtgcttc ggcattcaca acggg
//
LOCUS       DAZUCE010000701          324 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZUCE010000701 DAZUCE010000000
VERSION     DAZUCE010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.705"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>324)
                     /gene="tli1"
                     /locus_tag="ACHY03_005541"
     CDS             complement(<1..>324)
                     /gene="tli1"
                     /locus_tag="ACHY03_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFS3908600.1"
                     /translation="TRPSQVSVGRWNDHLGATESYYNKGKALPLMLRFCWDSIIDKKS
                     YETLIFFKKGTWEQMVMPYTDNRWNETYYRSTMIIGLAPEGKVRVWLGDRGNPVVPQL
                     DAKITT"
BASE COUNT           85 a           87 c           58 g           94 t
ORIGIN      
        1 gtagtaattt tagcatccaa ctgaggtacc accggattac cgcgatctcc cagccagact
       61 cttaccttcc cttcaggtgc cagaccaata atcatcgttg aacgataata ggtttcgttc
      121 cagcggttgt ctgtgtaagg cataaccatc tgctcccagg tccctttttt aaaaaaaatc
      181 agagtttcat aactcttttt atcaattatt gaatcccagc aaaaacgtaa catcagaggt
      241 aatgctttcc ccttgttata atacgactca gtcgcgccca gatggtcatt ccagcgccct
      301 acactgacct gactcggtct tgtg
//
LOCUS       DAZUCE010000702          319 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZUCE010000702 DAZUCE010000000
VERSION     DAZUCE010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.706"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            14..>319
                     /locus_tag="ACHY03_005542"
     CDS             14..>319
                     /locus_tag="ACHY03_005542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908601.1"
                     /translation="MAKAQKGSVINSITFTDSLTDETDDKHDFTNTGDLKGSTPSIMT
                     FPYGESDDRYTTNYVGNVQSSKTKFINLATGLTNDTTPTILISLDKELNNNQHIEITR
                     "
BASE COUNT          113 a           56 c           57 g           93 t
ORIGIN      
        1 cccagtcagt gtgatggcaa aagcacagaa aggtagcgtc attaattcca ttaccttcac
       61 tgatagttta actgatgaaa ccgatgataa acatgatttt actaatacag gtgatctgaa
      121 gggcagtacg ccttctatca tgacatttcc gtatggtgaa tcagatgata ggtatacgac
      181 taactatgtt ggtaatgttc agtcctcaaa aacaaaattt ataaatcttg caacagggtt
      241 aacgaatgat actacgccaa ctatattaat atctcttgat aaggagctga ataataatca
      301 acatattgaa attacacgt
//
LOCUS       DAZUCE010000703          318 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZUCE010000703 DAZUCE010000000
VERSION     DAZUCE010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.707"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>318
                     /locus_tag="ACHY03_005543"
     CDS             <1..>318
                     /locus_tag="ACHY03_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS3908602.1"
                     /translation="HALDVHPRLTALRVDLRLPDVPAATDAAVISRFINALKARIDAY
                     QKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVNRDTWCRAGDYRAPGSLAG
                     MIK"
BASE COUNT           67 a           95 c           92 g           64 t
ORIGIN      
        1 gcacgcactg gatgttcatc cccgcctgac ggcacttcgg gttgatctgc gtctcccgga
       61 tgtgccggca gcaacggacg cggctgtgat atcccgcttc atcaatgccc tgaaagcccg
      121 aatcgacgct tatcagaaac gtaagcatcg ggaaggtaaa cgcgtgcatt cctcgaccct
      181 gcattacgcc tgggcccggg agttcgggga gcttaaaggc aaaaaacact atcacctgct
      241 gctgctggtc aaccgggata cctggtgtcg tgccggcgat taccgcgctc cggggtcact
      301 ggccgggatg attaaaca
//
LOCUS       DAZUCE010000704          317 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZUCE010000704 DAZUCE010000000
VERSION     DAZUCE010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.708"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..240)
                     /locus_tag="ACHY03_005544"
     CDS             complement(<1..240)
                     /locus_tag="ACHY03_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908603.1"
                     /translation="MDERFINNTPTSLSFKHADLSGLSYKNARLELTLICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYIVAN"
BASE COUNT           95 a           68 c           55 g           99 t
ORIGIN      
        1 attggctact atatagatcc ccgtttggtt aattccctgc tttcctgaca tacctaacaa
       61 atcaccttcg tcagtaacac gaaatgagtg tatccagtca aatgatatct taacttctga
      121 cttgatcgcc gggcatatca gggtaagttc aagcctggca ttcttatagc ttaaacctga
      181 cagatcggcg tgtttaaaag acaacgaagt cggcgtgtta tttataaaac gttcatccat
      241 gttaacatcc ttaatatatg agactgtact caatttaacc ttctacaaaa atggtgcgag
      301 tactgtttaa gtaccat
//
LOCUS       DAZUCE010000705          315 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZUCE010000705 DAZUCE010000000
VERSION     DAZUCE010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.709"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..120
                     /locus_tag="ACHY03_005545"
     CDS             <1..120
                     /locus_tag="ACHY03_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFS3908604.1"
                     /translation="QAKITTSAARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            101..>315
                     /locus_tag="ACHY03_005546"
     CDS             101..>315
                     /locus_tag="ACHY03_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFS3908605.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVV"
BASE COUNT           74 a           73 c           88 g           80 t
ORIGIN      
        1 caggcaaaga taacgacttc agcggcacgc gtaatgacca tccgtctggg gcgttatcag
       61 cgggttcagg aacattatca acgtaaggag gctcaggaaa atggcgatta tcgctggtaa
      121 ggctggttac ggtctgatta ttgcgttgtt ttccctgtcg ttatcagggt gtggcctgac
      181 gcaaagagtg gcagacggta cggtatctgc cacgaaatcc ctgttttacc gacagataaa
      241 aacacttcac cttgatattc gtgccaggga ggccattaac accagtgctg ccggtattcc
      301 cctgtcggtt gtggt
//
LOCUS       DAZUCE010000706          314 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZUCE010000706 DAZUCE010000000
VERSION     DAZUCE010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.710"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>314
                     /locus_tag="ACHY03_005547"
     CDS             <1..>314
                     /locus_tag="ACHY03_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFS3908606.1"
                     /translation="SEKSHGREEIRLHIVCDVPDELIDFTFELKGLKKLCMAVSFRSI
                     IAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCRIRRG
                     NAA"
BASE COUNT          109 a           58 c           75 g           72 t
ORIGIN      
        1 agtgaaaaga gtcacggcag agaagaaatc cgtcttcata ttgtttgcga tgtccctgat
       61 gaacttattg atttcacgtt tgaattgaaa ggactaaaaa aattatgcat ggcagtctcc
      121 tttcggtcaa taatagcaga acaaaagaaa gaacccgaaa tgacggtcag atattatatc
      181 agctctgctg atttaactgc agagaagttc gccacagcga tccgaaatca ctggcacgtg
      241 gaaaataagc tgcactggcg cctggacgtg gtaatgaatg aagacgactg cagaataaga
      301 agaggaaatg cagc
//
LOCUS       DAZUCE010000707          314 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZUCE010000707 DAZUCE010000000
VERSION     DAZUCE010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.711"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..141)
                     /locus_tag="ACHY03_005548"
     CDS             complement(<1..141)
                     /locus_tag="ACHY03_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_302948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908607.1"
                     /translation="MANNILHSSTSDSESKNTSTDSTLFNEKFPFQLRQDFASRITED
                     ESD"
     gene            complement(162..>314)
                     /locus_tag="ACHY03_005549"
     CDS             complement(162..>314)
                     /locus_tag="ACHY03_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFS3908608.1"
                     /translation="ISDIHVAISHIVVDNSVQPEESVSFSYESIIWEHCSAGTSAYSL
                     WEDRLF"
BASE COUNT           95 a           55 c           53 g          111 t
ORIGIN      
        1 atcactttca tcttctgtta ttcttgatgc gaaatcttgt cttagttgaa atgggaattt
       61 ttcattaaat aacgtactgt cagtgcttgt atttttgctt tcactgtctg atgttgaaga
      121 gtgtaaaata ttgttagcca ttataataat cccatatgtt tttaaaaaag acgatcttcc
      181 cataaactgt atgcactagt gccagcggaa caatgctccc atataattga ctcataactg
      241 aaagaaactg actcttcggg ttgaacgctg ttatcaacaa caatatgtga tatagcgaca
      301 tgaatgtcgc ttat
//
LOCUS       DAZUCE010000708          312 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZUCE010000708 DAZUCE010000000
VERSION     DAZUCE010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.712"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>312)
                     /locus_tag="ACHY03_005550"
     CDS             complement(<1..>312)
                     /locus_tag="ACHY03_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908609.1"
                     /translation="GVSFISMMILSRVYDFLLPTQDTFPGDIIRSLCVVCPSILIWFQ
                     FSVYVMRKYYRHILLRGKETETISERLIREADTREYELWRPVRRFFSIIFLIVLLVCI
                     HS"
BASE COUNT          112 a           60 c           60 g           80 t
ORIGIN      
        1 tgaatgaatg caaactaata gaacaatgag aaaaataatg gagaaaaagc gacggaccgg
       61 acgccatagt tcatattctc gtgtatcagc ctcacgtatc aacctttctg aaatggtttc
      121 ggtttctttt cctcgcagca gaatatgtct gtaatatttc ctcatgacat ataccgaaaa
      181 ctgaaaccag attaatatgg atggacacac aacacataaa cttctgatga tatcacccgg
      241 gaaagtatct tgggtgggta aaagaaagtc atatactctg gataaaatca tcatcgagat
      301 aaatgagacg cc
//
LOCUS       DAZUCE010000709          305 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZUCE010000709 DAZUCE010000000
VERSION     DAZUCE010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.713"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            108..229
                     /locus_tag="ACHY03_005551"
                     /pseudo
     CDS             108..229
                     /locus_tag="ACHY03_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           73 a           62 c           79 g           91 t
ORIGIN      
        1 aggtctttga atttgttagc cagatttctg ttctaatttt ggatagcaga atatgcaatg
       61 ttatttttcg aatcctccat aactgatata tgtgctgtta gtggagtaca agaataagct
      121 cgcgttagca gtctgtcagt actgcgacag cgttgtctgg gagggcttta cccggtcaaa
      181 acagcattat tgtgcacccc atgtgacgga tgaactgtgt gctcaggggg cattttactg
      241 taaaaaccgt gctgcggtca gccgtaaccg tcaggtgggt gaacgcaaca gtcgcgccat
      301 tgctt
//
LOCUS       DAZUCE010000710          305 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZUCE010000710 DAZUCE010000000
VERSION     DAZUCE010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.714"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>305
                     /locus_tag="ACHY03_005552"
     CDS             <1..>305
                     /locus_tag="ACHY03_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908610.1"
                     /translation="VTSKTDGVADAPALTIPEVADSVANAAELKDGLQAEVKLPAGTV
                     EGAVITLTVTHPDKTTRTETHTVSKDEAADGTVSMVVPKGSVVDGQNSVSVSLTQGS"
BASE COUNT           83 a           62 c           95 g           65 t
ORIGIN      
        1 gtgacgtcaa aaactgatgg tgtggcagat gctccggccc tgaccattcc agaagtggct
       61 gatagtgtgg ccaatgcggc tgagctgaaa gatggtttac aggcagaagt caagttgccg
      121 gcaggaacag tggaaggtgc tgtcattaca ctgacagtga ctcatccgga taaaaccacc
      181 agaaccgaga cgcatactgt tagcaaagat gaggctgcag acggtactgt gtcaatggtt
      241 gttccgaagg gaagtgttgt tgacggtcag aacagtgtga gtgtcagcct gactcagggc
      301 agtaa
//
LOCUS       DAZUCE010000711          300 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZUCE010000711 DAZUCE010000000
VERSION     DAZUCE010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.715"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>300
                     /locus_tag="ACHY03_005553"
     CDS             <1..>300
                     /locus_tag="ACHY03_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908611.1"
                     /translation="KEAEAALTQAKADNKITQQEHDDLAAKNDAVTAAKADAAKAVEG
                     LPAGDAKDGLNGRLAKVDGIDVPAVDENGNGKPDAEEAAEAVNAATAKVAEAEAK"
BASE COUNT           91 a           62 c          113 g           34 t
ORIGIN      
        1 aaaaagaagc agaagcggcg ctgactcagg cgaaggctga caataagatc acccagcagg
       61 agcatgatga tcttgcggcg aagaacgatg cggtaacggc ggcgaaagca gatgcggcga
      121 aagcggtgga aggtctgccg gccggagatg cgaaggacgg tctgaacggg cgtctggcta
      181 aagttgacgg tatcgatgta ccggcagtgg atgaaaacgg caacggcaag ccggatgcag
      241 aggaagcagc agaggcggtg aatgcagcga cagccaaggt agcagaagcg gaagcgaagg
//
LOCUS       DAZUCE010000712          298 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZUCE010000712 DAZUCE010000000
VERSION     DAZUCE010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.716"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>298
                     /locus_tag="ACHY03_005554"
     CDS             <1..>298
                     /locus_tag="ACHY03_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFS3908612.1"
                     /translation="KVLLNDKNNELSDGTNFWDKNRQLTTDEIACYLKKIAANAKNTQ
                     VNYPTDFYLPNSNSTYLEVALNDNIKSDPSWPKEVQLFPINTGGHWILVSLQKIV"
BASE COUNT          108 a           64 c           50 g           76 t
ORIGIN      
        1 caaaggtact tttaaatgat aagaataatg aactgagcga tgggacaaac ttctgggata
       61 aaaatcgtca attaaccaca gatgaaatag cttgctatct taaaaagatc gccgccaatg
      121 caaaaaatac tcaagtcaat tatcctactg atttctacct ccccaattcc aacagcactt
      181 acctggaagt cgctctcaat gataatatta agagcgatcc atcatggccg aaagaagtcc
      241 agttattccc cataaatact ggcggacact ggatattagt ttcgctacag aaaatagt
//
LOCUS       DAZUCE010000713          295 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZUCE010000713 DAZUCE010000000
VERSION     DAZUCE010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.717"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>295
                     /locus_tag="ACHY03_005555"
     CDS             <1..>295
                     /locus_tag="ACHY03_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFS3908613.1"
                     /translation="VGDPGVGKTALVEGLALRIAEGNVPESLRPVTLRTLDLGLLQAG
                     AGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAANLLKPA"
BASE COUNT           56 a           83 c           98 g           58 t
ORIGIN      
        1 tggtggggga ccctggtgtg gggaaaacgg ctctggtgga agggctcgcc ctgcgtattg
       61 cggaaggtaa cgtaccggaa tcactcagac ccgtcaccct gcgcaccctt gacctcggcc
      121 tgctgcaggc cggtgccggc gtgaagggtg aatttgaaca gcgcctgaaa aacgtgattg
      181 atgccgtgca gcagtcaccg gctcctgtac tgttgtttat agatgaagcc cataccctta
      241 tcggggccgg taatcaggcc ggcggcgcgg atgcggccaa cctgctgaag cctgc
//
LOCUS       DAZUCE010000714          293 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZUCE010000714 DAZUCE010000000
VERSION     DAZUCE010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.718"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>293
                     /locus_tag="ACHY03_005556"
     CDS             <1..>293
                     /locus_tag="ACHY03_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908614.1"
                     /translation="WAERYLVISASSVMLPGGVTAGAILKSLADTLPSGYLLTKRLKN
                     INTPVTSALASLQLSICQLPAALPVNVTLITDQPDSEIRSAFVSAWEALFPQRV"
BASE COUNT           70 a           71 c           65 g           87 t
ORIGIN      
        1 tgggcagaga ggtatctggt tatctccgca agcagtgtta tgcttcctgg tggtgtgaca
       61 gccggagcaa ttctgaaatc actggcagat acattacctt ccgggtactt gctgacaaaa
      121 cgcttaaaga atattaatac acctgtaact tctgccctgg caagcttgca actatctatt
      181 tgtcagttac ctgccgctct ccctgtaaat gtgacgctga taaccgatca gcctgattct
      241 gaaatcagat ctgcttttgt ttctgcatgg gaagcgttat ttccccaacg cgt
//
LOCUS       DAZUCE010000715          287 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZUCE010000715 DAZUCE010000000
VERSION     DAZUCE010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.719"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>287)
                     /locus_tag="ACHY03_005557"
     CDS             complement(<1..>287)
                     /locus_tag="ACHY03_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908615.1"
                     /translation="ANVGIPADKVTQDGEYTVKAEITDPAGNTSGQGKATQFGVDTVA
                     PSEPALKAENDGSVSATLPDGANKGDKVEVTFTDEEGNEQKVTLEKGDGNW"
BASE COUNT           52 a           95 c           55 g           85 t
ORIGIN      
        1 ctccagttgc catccccttt ctccagcgtg actttctgct cattaccctc ttcatccgta
       61 aaggtcactt ccaccttgtc acctttgttc gcaccatctg gcagagtcgc tgatacagaa
      121 ccatcattct cagccttcag tgctggttct gacggagcta cagtatccac gccaaactgg
      181 gtagctttac cctgaccact ggtgttgcct gccgggtctg tgatttcagc cttaactgtg
      241 tattccccat cctgcgtcac tttatccgca ggaatgccca cattcgc
//
LOCUS       DAZUCE010000716          286 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZUCE010000716 DAZUCE010000000
VERSION     DAZUCE010000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.720"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>286
                     /locus_tag="ACHY03_005558"
     CDS             <1..>286
                     /locus_tag="ACHY03_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908616.1"
                     /translation="KLSTLMPSEDEKKRRDEQFAAFDDYCRKVMSRNLAECFSIHNDN
                     FSELEWECNRPSFVVSGDGVKITISENGKVTPPSHQHSEELIEFAIDYLKN"
BASE COUNT           87 a           54 c           71 g           74 t
ORIGIN      
        1 aaaactgtcg acgctgatgc cttctgaaga tgaaaaaaaa cgccgcgatg agcagtttgc
       61 tgcgtttgac gattattgtc ggaaagtgat gagcagaaat ctcgcagagt gtttcagtat
      121 tcataatgat aatttcagtg agctggaatg ggagtgtaac cggccatcct ttgttgtatc
      181 cggtgatggt gtgaaaataa ccatctcaga aaatgggaaa gtaacacctc catcgcacca
      241 gcacagtgag gagctcattg aatttgccat tgattacctg aagaac
//
LOCUS       DAZUCE010000717          284 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZUCE010000717 DAZUCE010000000
VERSION     DAZUCE010000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.721"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>284)
                     /locus_tag="ACHY03_005559"
     CDS             complement(<1..>284)
                     /locus_tag="ACHY03_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFS3908617.1"
                     /translation="GRVSVRFKVPGALVPNQFALGIYWQLSSLPEGVTLSEEGNDMLM
                     SFFLQTDATNLNAMYHKKQNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPV"
BASE COUNT           63 a           93 c           66 g           62 t
ORIGIN      
        1 accggcgtca cctgaatgct gttgtttccg gcaaactcaa aagccagtgt gtgccatccg
       61 ttatcaaaga ccccgaacgt atccagcttc gcattctgct tcttgtggta catcgcgttc
      121 aggttcgtcg catccgtctg caggaagaag gacatcagca tgtcgttgcc ttcctctgac
      181 agcgtcactc cctccggcag ggacgacaac tgccagtaaa tacccagggc aaactgattc
      241 ggcaccagtg cgcccggcac cttaaaccgc acgctcacac gtcc
//
LOCUS       DAZUCE010000718          284 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZUCE010000718 DAZUCE010000000
VERSION     DAZUCE010000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.722"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>284
                     /locus_tag="ACHY03_005560"
     CDS             <1..>284
                     /locus_tag="ACHY03_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFS3908618.1"
                     /translation="DYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEERLRIAL
                     LNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSL"
BASE COUNT           69 a           66 c           79 g           70 t
ORIGIN      
        1 gactatcttg cggataaata cagttttact gaactgaatg aacttccacg ccttcaccag
       61 cagtggcagg atgttctgga ggagtgccgg cagacagagg ccgggccgga agaacggctg
      121 cgtattgcac tgctgaatgt ggattacgtc accagttttg aactgccttt tcgcttgttg
      181 cttacccgga caccacaact gattgccgcg ctgcgtgaag aatggggcat cagccagaaa
      241 aatgtggtgt tcaatgataa acgatttggc tgcgtgtaca gcct
//
LOCUS       DAZUCE010000719          281 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZUCE010000719 DAZUCE010000000
VERSION     DAZUCE010000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.723"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
BASE COUNT           94 a           58 c           53 g           76 t
ORIGIN      
        1 tagatataac ctcatatcaa ggtgtcactg gtaaataatc ggataaaaga cagtatcggt
       61 cggcgcatag agcttcttct atttcaccag aagtgcacag caggaactac caatcacacg
      121 gttagtgtca tgggtggagg atgcggcccg ttctttccct ataacagggt tacgcagttt
      181 caatcagtac atgcagaaaa acccctaatt cacatcactg cagaaagcat ttaatagaaa
      241 tatacatcgt ttaagaattt caattaaatc aagattattc t
//
LOCUS       DAZUCE010000720          279 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZUCE010000720 DAZUCE010000000
VERSION     DAZUCE010000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.724"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..215)
                     /locus_tag="ACHY03_005561"
     CDS             complement(<1..215)
                     /locus_tag="ACHY03_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HFS3908619.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNH"
BASE COUNT           69 a           79 c           74 g           57 t
ORIGIN      
        1 tcatggttta ccagtgttcc gccattcact gtttcacccg ggtgcacaac gatgtcagca
       61 gccagcaccg ggagtgacgt gaccgcggca agagacagtg caaccgccac accgccacgt
      121 ttaccccgtg cgcgggccag ttcggaggca accacgaaag cgcccgtaat gtgattccat
      181 accagcctgt agcaggtatt cagatgtcgt ttcatcagca tttccttaat cagagtgagg
      241 gtgaataaaa aagccggtat ccacagatgt ggataccgg
//
LOCUS       DAZUCE010000721          278 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZUCE010000721 DAZUCE010000000
VERSION     DAZUCE010000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.725"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>278)
                     /locus_tag="ACHY03_005562"
     CDS             complement(<1..>278)
                     /locus_tag="ACHY03_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFS3908620.1"
                     /translation="ELPNKETISSNFCERLEQWGNKSLNNGEERAIAVERIKEAYNSN
                     MASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDFTDNASLVNINLS"
BASE COUNT           81 a           51 c           56 g           90 t
ORIGIN      
        1 agctgaggtt gatattaacg agactggcat tgtcagtaaa gtcaagacaa gttagacaat
       61 tgttttccag atttaacgta ttaactgtcg aggggatagg cggtaactca ctcaaatcaa
      121 gatatgacaa atcaagagat gccatgttcg aattgtaagc ttctttaatt cgctccacgg
      181 caattgctct ttcttcacca ttattaagcg acttattacc ccattgttct agtcgttcac
      241 aaaaattgct ggagatggtt tctttgttag gtaattcg
//
LOCUS       DAZUCE010000722          275 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZUCE010000722 DAZUCE010000000
VERSION     DAZUCE010000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.726"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>275)
                     /locus_tag="ACHY03_005563"
     CDS             complement(<1..>275)
                     /locus_tag="ACHY03_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908621.1"
                     /translation="DGDFNILTSDEYMSIMTPLMKCERSIHDHNRYVTGKDTLLLDYP
                     PSGNQIYFHIFPDESATLVLYFSNNDIECFVFERDIPSQYRFFKMFT"
BASE COUNT           87 a           48 c           58 g           82 t
ORIGIN      
        1 tggtaaacat tttgaaaaag cgatactggc tcggaatatc tctttcaaag acgaaacatt
       61 caatgtcatt gtttgaaaaa taaaggacta gtgttgctga ttcgtcagga aaaatatgga
      121 aatatatctg gttaccagaa ggaggatagt ccagaagaag ggtatctttg cccgtcacat
      181 acctgttgtg gtcgtggatg ctgcgctcac acttcatcag aggtgtcatg atactcatgt
      241 attcatctga tgttaatata ttaaaatcac catcc
//
LOCUS       DAZUCE010000723          272 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZUCE010000723 DAZUCE010000000
VERSION     DAZUCE010000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.727"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>272)
                     /locus_tag="ACHY03_005564"
     CDS             complement(<1..>272)
                     /locus_tag="ACHY03_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFS3908622.1"
                     /translation="RWAVSKETFTKEKFDSYVESVFSKIDFYKQCGVINPQNANTAYF
                     GDTDGRVGAVLYALLVSGHIGIREKGWSLLCDLLKHEDMASFAYEN"
BASE COUNT           84 a           66 c           39 g           83 t
ORIGIN      
        1 attttcatat gcgaatgaag ccatgtcttc gtgttttaat aaatcacata ataaactcca
       61 ccccttttcc ctgatgccta tatgccctga aacgagcaat gcataaagta cagcgcctac
      121 tcttccatct gtatcgccaa aataagccgt gttggcattt tgtgggttaa tcacaccaca
      181 ctgtttataa aagtctattt ttgaaaaaac agattcaaca tagctatcaa acttttcctt
      241 agtaaacgtc tctttagaga cagcccaacg cc
//
LOCUS       DAZUCE010000724          268 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZUCE010000724 DAZUCE010000000
VERSION     DAZUCE010000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.728"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            22..>268
                     /locus_tag="ACHY03_005565"
     CDS             22..>268
                     /locus_tag="ACHY03_005565"
                     /inference="COORDINATES: protein motif:HMM:NF017942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFS3908623.1"
                     /translation="MSAGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRA
                     RNDQHQFDGYVTYKVNDSWLFAWQTTVYTKVNNFKYGN"
BASE COUNT           82 a           50 c           63 g           73 t
ORIGIN      
        1 ccagggattc agacacaatg gatgtcagca ggaagtcagt ataaccctta tctgaaaatt
       61 tcgtatcagg caacgccaga tcttaatctg gccttacgct atcgctatga ctggaaagct
      121 ttccgacaga cggatttaga tggcaacagg gcgcgtaatg atcagcatca gtttgatggt
      181 tatgtgacat acaaagttaa tgattcatgg ttgtttgcat ggcagaccac ggtatatacg
      241 aaggttaata atttcaaata tgggaatc
//
LOCUS       DAZUCE010000725          266 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZUCE010000725 DAZUCE010000000
VERSION     DAZUCE010000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.729"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(98..>266)
                     /locus_tag="ACHY03_005566"
     CDS             complement(98..>266)
                     /locus_tag="ACHY03_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFS3908624.1"
                     /translation="GCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKESTKAYG
                     TGERNFGCSRG"
BASE COUNT           60 a           79 c           73 g           54 t
ORIGIN      
        1 gtgaatggcg gttttcagtt cagtctcaga agggaaaatc agtgtggtcg ctgtgaacac
       61 gtcatcacac taaggtcaga taagtcgtct gtcgggctca gccacgactg cagccaaagt
      121 tgcgttcacc ggtgccgtaa gctttggtac tctccttcgc cagatagtca gcacgctcca
      181 gcacctgctg aaagccggtg tcatcattac gcgccagcca caccgccggc caggccggaa
      241 aatgcaccag cgtggcatgg cacccg
//
LOCUS       DAZUCE010000726          260 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZUCE010000726 DAZUCE010000000
VERSION     DAZUCE010000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.730"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>260
                     /locus_tag="ACHY03_005567"
     CDS             <1..>260
                     /locus_tag="ACHY03_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908625.1"
                     /translation="QMTAVHREEGISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDL
                     TAVITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRVI"
BASE COUNT           62 a           76 c           87 g           35 t
ORIGIN      
        1 cagatgacgg cggtccaccg cgaggaaggc atcagccttt accgccgcta tgacaaccgt
       61 ggccggttaa cctcggtgaa agacgcacag ggccgtgaaa cgcggtatga atacaacgcc
      121 gcaggcgacc tgactgccgt tatcaccccg gacggcaacc ggagcgagac acagtacgat
      181 gcgtggggaa aggcggtcag caccacgcag ggcgggctga cgcgcagtat ggagtacgat
      241 gctgccggac gtgtcatcag
//
LOCUS       DAZUCE010000727          260 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.731, whole genome shotgun sequence.
ACCESSION   DAZUCE010000727 DAZUCE010000000
VERSION     DAZUCE010000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.731"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>260
                     /locus_tag="ACHY03_005568"
     CDS             <1..>260
                     /locus_tag="ACHY03_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFS3908626.1"
                     /translation="DTAKIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVN
                     CAALPESLLESELFGHEKGAFTGAQTLRQGLFERANEGTLLL"
BASE COUNT           65 a           63 c           73 g           59 t
ORIGIN      
        1 gacaccgcca aaattgccct ttctcaggcc agcgtcttga ttagcggtga aagcggcacc
       61 gggaaagagt tgattgccag agcgattcac tacaattcgc ggcgggcaaa ggggccgttc
      121 attaaagtca actgcgcggc gctgccggaa tcgttgctcg aaagtgaact gtttggtcat
      181 gaaaaaggtg catttactgg tgcacaaacc ttgcgtcagg gattatttga acgtgccaac
      241 gaaggtactc tgctcctcga
//
LOCUS       DAZUCE010000728          258 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZUCE010000728 DAZUCE010000000
VERSION     DAZUCE010000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.732"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            38..>258
                     /locus_tag="ACHY03_005569"
     CDS             38..>258
                     /locus_tag="ACHY03_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HFS3908627.1"
                     /translation="MSGAYASVIDGNIFLAGGAYFPGAAEKYSNGEYWSHKGLDKAYS
                     KEIYQLIKNDWKKVGSLPEGLAYGVSLPW"
BASE COUNT           74 a           48 c           68 g           68 t
ORIGIN      
        1 atggtctgaa cttccaccag tagcttcacc cgagggggtg tcaggagcat atgcctctgt
       61 tattgatgga aatatctttc tggccggtgg tgcatatttc ccaggggcgg cagaaaagta
      121 tagcaatggt gaatactggt ctcataaagg actggataaa gcctattcaa aagagatata
      181 tcagctaata aaaaatgact ggaaaaaagt tggttctttg ccggaaggac ttgcttatgg
      241 tgtaagcctg ccctggca
//
LOCUS       DAZUCE010000729          257 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZUCE010000729 DAZUCE010000000
VERSION     DAZUCE010000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.733"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>257)
                     /locus_tag="ACHY03_005570"
     CDS             complement(<1..>257)
                     /locus_tag="ACHY03_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFS3908628.1"
                     /translation="GAGIEDADGQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLF
                     MNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNADISGH"
BASE COUNT           51 a           80 c           72 g           54 t
ORIGIN      
        1 atatgtccgc tgatgtcagc attgcgggcc gtcagcctgc cctccggcgt cagggagaac
       61 gtcgggggat tgccggacga ggtgatgctc actgcaaaca gtcgcttcag gaacacatcg
      121 ttcatgaaca gctgattccc ctgcgccaca aacagcggcg tggtgttgcc gttctccggg
      181 ttaatcatcg cgatacggtc cgcctgcagc agtatattgc tcaggggctg accatcagca
      241 tcctcaatcc ccgcacc
//
LOCUS       DAZUCE010000730          256 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.734, whole genome shotgun sequence.
ACCESSION   DAZUCE010000730 DAZUCE010000000
VERSION     DAZUCE010000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.734"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..195
                     /locus_tag="ACHY03_005571"
     CDS             <1..195
                     /locus_tag="ACHY03_005571"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908629.1"
                     /translation="LHIIGGAGENTILGTMHQVLLPLKQDHPGTRWWCSALEMVPSGE
                     LSLFSMLFAYDIHQVNLINS"
BASE COUNT           71 a           47 c           61 g           77 t
ORIGIN      
        1 ctgcacatta ttggaggggc aggggaaaac accattttgg gaacaatgca tcaggtactc
       61 ttaccactga agcaagatca tccgggcacc aggtggtggt gcagtgcttt ggaaatggtc
      121 ccctcggggg agttgtcatt attttctatg ctatttgctt atgatattca tcaggtgaat
      181 ttaataaatt cctaatttga aatatccact attaagccag tgtttaacga cgttaagatg
      241 gcaatgtgca gatcat
//
LOCUS       DAZUCE010000731          255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.735, whole genome shotgun sequence.
ACCESSION   DAZUCE010000731 DAZUCE010000000
VERSION     DAZUCE010000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.735"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>255
                     /locus_tag="ACHY03_005572"
     CDS             <1..>255
                     /locus_tag="ACHY03_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HFS3908630.1"
                     /translation="INGKKNNSNLSDVFIPYVNTFSDLKNKILRMKIELTEGSGFSKS
                     LSDSQIEMHILRTVNSLNVGEKLNDDNLADHSIFTNEFSV"
BASE COUNT           87 a           37 c           44 g           87 t
ORIGIN      
        1 atattaatgg gaagaaaaac aactctaatt tgtcagatgt ttttattccc tatgtaaata
       61 cattttccga cctcaaaaat aaaatactca ggatgaaaat tgaactgact gaaggtagtg
      121 gtttttctaa aagtctttct gacagtcaga ttgagatgca tattctgagg actgttaatt
      181 cgctaaatgt tggcgagaaa ttgaatgatg ataatcttgc agatcattct atattcacta
      241 acgaattctc tgtta
//
LOCUS       DAZUCE010000732          255 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.736, whole genome shotgun sequence.
ACCESSION   DAZUCE010000732 DAZUCE010000000
VERSION     DAZUCE010000732.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.736"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..251)
                     /locus_tag="ACHY03_005573"
     CDS             complement(<1..251)
                     /locus_tag="ACHY03_005573"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFS3908631.1"
                     /translation="MGKNPDAPIASIGAVFFDPQTGEQGPEFSKIIDMGTCGGTVDIS
                     TIEWWLQRSGEARAAILADRIPLDDALLQLREFIDENSG"
BASE COUNT           59 a           62 c           59 g           75 t
ORIGIN      
        1 tcaccggagt tttcgtctat aaattcccgt aattgtaaaa gcgcatcatc aagcggtatt
       61 cgatcagcta aaatggcggc acgggcttcg ccagagcgtt gaagccacca ttcgatggtg
      121 cttatgtcta cagtgccgcc acatgtaccc atatcaatta ttttgctgaa ttcaggcccc
      181 tgctctccgg tttgtggatc gaaaaataca gcgcctattg aggctattgg tgcgtcagga
      241 tttttgccca ttgtt
//
LOCUS       DAZUCE010000733          252 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.737, whole genome shotgun sequence.
ACCESSION   DAZUCE010000733 DAZUCE010000000
VERSION     DAZUCE010000733.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.737"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            25..>252
                     /locus_tag="ACHY03_005574"
     CDS             25..>252
                     /locus_tag="ACHY03_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFS3908632.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVL"
BASE COUNT           67 a           59 c           74 g           52 t
ORIGIN      
        1 gttgtaatta tccatggagt cgaaatggaa aaaacacgta agcgccgtgg taccggacgg
       61 gtgacgctgc aggaggtcgc taactttgca ggtgtcggaa ccatgaccgt ttcccgcgcg
      121 ctgagaacac cggaacaggt ttctgacaag ctgagggaaa agatagaaca ggccgtagaa
      181 gagttgggtt atattcccaa cagaactgct ggcgcccttg catctggtca cagtcataca
      241 gtcgccgtac tg
//
LOCUS       DAZUCE010000734          247 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.738, whole genome shotgun sequence.
ACCESSION   DAZUCE010000734 DAZUCE010000000
VERSION     DAZUCE010000734.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.738"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
BASE COUNT           93 a           60 c           36 g           58 t
ORIGIN      
        1 caaggagaat ccctcctaca aaaaaacaac attagaaaca gtaatacatt agctatgtat
       61 tcttatgcac acctagatag gttatattat aacattcaga tgaatcaccc tgcgacaaac
      121 ttctcatcaa agaaatatca aacaggatgc agcaaagtaa aacccaccta ttaataaaaa
      181 agccccggac attccggggc tacactgcat attaacgaac gctctgtccg gttatttcaa
      241 ccctgcg
//
LOCUS       DAZUCE010000735          246 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.739, whole genome shotgun sequence.
ACCESSION   DAZUCE010000735 DAZUCE010000000
VERSION     DAZUCE010000735.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.739"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>246)
                     /locus_tag="ACHY03_005575"
     CDS             complement(<1..>246)
                     /locus_tag="ACHY03_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure C-terminal
                     domain-containing protein"
                     /protein_id="HFS3908633.1"
                     /translation="AQVTGALKQTWADEDMLRGDWLAGLKSGWGEWAESATDSFSQVK
                     SAATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTE"
BASE COUNT           44 a           92 c           56 g           54 t
ORIGIN      
        1 ttctgtcatc atggacagca ccgaacgggt gaatccccgc cagtctgcct ctgcaccggt
       61 cagcatcgcc gccatattct gtgcaatacc gtcaaaggtc tgcgtggcag cacttttaac
      121 ctgcgaaaaa ctgtccgtcg cactttccgc ccactccccc cagccggact tcagcccggc
      181 cagccagtca ccgcgcagca tgtcttcatc cgcccatgtc tgtttcagtg ccccggtgac
      241 ctgtgc
//
LOCUS       DAZUCE010000736          245 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.740, whole genome shotgun sequence.
ACCESSION   DAZUCE010000736 DAZUCE010000000
VERSION     DAZUCE010000736.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.740"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(<1..>245)
                     /locus_tag="ACHY03_005576"
     CDS             complement(<1..>245)
                     /locus_tag="ACHY03_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFS3908634.1"
                     /translation="FEVILTGARTYIFSHTTSRIDVELGAKLFRHLLALPVSYFENRR
                     VGETVARVRELEQIRNFLTGQALTSVLDLFFSVIFFC"
BASE COUNT           90 a           52 c           41 g           62 t
ORIGIN      
        1 acaaaaaaat attacagaaa aaaacagatc aagaactgat gtcaacgctt gtccggttaa
       61 aaaattacga atttgctcca gttcccttac tctggcaacg gtctctccga ccctgcgatt
      121 ttcaaaatat gaaacaggca atgcaagcaa atgtctgaat aacttagcac ccagttcgac
      181 gtcaatacga cttgtagtat gagagaaaat ataagttctg gctccggtta atatcacttc
      241 aaaaa
//
LOCUS       DAZUCE010000737          243 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.741, whole genome shotgun sequence.
ACCESSION   DAZUCE010000737 DAZUCE010000000
VERSION     DAZUCE010000737.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.741"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>243
                     /locus_tag="ACHY03_005577"
     CDS             <1..>243
                     /locus_tag="ACHY03_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFS3908635.1"
                     /translation="QPAIKNGERVPATTNGPFKVNGKWTSGTHYTGGSKRLDAGIIDI
                     TSPTNQYGLHPVIEGFDITLNKTKPSAVDIYSDVFG"
BASE COUNT           85 a           45 c           47 g           66 t
ORIGIN      
        1 ttcaaccagc cataaaaaat ggagagcgag ttcctgcgac aacgaatgga ccatttaaag
       61 taaatggtaa gtggacatcc ggcactcatt atacaggtgg ttccaaacgt ctagatgccg
      121 gtattattga tatcacatct cctacaaacc aatatggatt acatccagtt attgaaggat
      181 ttgatataac acttaataaa acaaaaccat cagcagtgga tatatattca gatgtgtttg
      241 gtg
//
LOCUS       DAZUCE010000738          237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.742, whole genome shotgun sequence.
ACCESSION   DAZUCE010000738 DAZUCE010000000
VERSION     DAZUCE010000738.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.742"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            complement(25..>237)
                     /locus_tag="ACHY03_005578"
     CDS             complement(25..>237)
                     /locus_tag="ACHY03_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFS3908636.1"
                     /translation="LSLKRAEAVRDWMRDTGDIPQSCFAVQGYGESRPVAPNETAEGR
                     ARNRRVEISLVPQADACRLPDITRAP"
BASE COUNT           41 a           83 c           64 g           49 t
ORIGIN      
        1 cctctcgttg tcttacgcgc cctgttacgg cgcccgggtg atgtccggca gacgacaggc
       61 atcggcctgc ggtaccagac tgatttcaac acggcggtta cgcgctcgcc cttccgccgt
      121 ctcattcgga gccacgggac gactctcacc atatccctgt acggcaaaac agctttgagg
      181 gatatcgccc gtatcccgca tccagtcgcg aacggcctct gcgcgcttca gtgacag
//
LOCUS       DAZUCE010000739          237 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.denovo.743, whole genome shotgun sequence.
ACCESSION   DAZUCE010000739 DAZUCE010000000
VERSION     DAZUCE010000739.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.denovo.743"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..>237
                     /locus_tag="ACHY03_005579"
     CDS             <1..>237
                     /locus_tag="ACHY03_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFS3908637.1"
                     /translation="DAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYWAGY
                     PSAQPFINAKEASPWKNEPFMRPINSNYARYCAK"
BASE COUNT           80 a           49 c           40 g           68 t
ORIGIN      
        1 tgatgctaaa tacaatacgc aatttagttt aaattatgaa ttaaaaaaat catttgtttg
       61 tccaattaat gatgaaaccg tattaattca ttatgtcggc ccgacaaaac catggcatta
      121 ctgggccggt tatccaagtg cgcaaccttt tatcaatgcc aaagaagcat cgccctggaa
      181 aaatgaaccg tttatgcggc caattaactc aaactatgct cgttattgcg ccaagca
//
LOCUS       DAZUCE010000740         6075 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.guided.268, whole genome shotgun sequence.
ACCESSION   DAZUCE010000740 DAZUCE010000000
VERSION     DAZUCE010000740.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.guided.268"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..194
                     /locus_tag="ACHY03_005580"
     CDS             <1..194
                     /locus_tag="ACHY03_005580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFS3908638.1"
                     /translation="TRRGVASALLKPWIKSESELTVDASITAKPFFERYGFQTVKQQR
                     VECRGAWFTNFYMRYKPQH"
     gene            440..643
                     /locus_tag="ACHY03_005581"
                     /pseudo
     CDS             440..643
                     /locus_tag="ACHY03_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            642..1163
                     /gene="prfH"
                     /locus_tag="ACHY03_005582"
                     /pseudo
     CDS             642..1163
                     /gene="prfH"
                     /locus_tag="ACHY03_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1220..2677)
                     /gene="pepD"
                     /locus_tag="ACHY03_005583"
     CDS             complement(1220..2677)
                     /gene="pepD"
                     /locus_tag="ACHY03_005583"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFS3908639.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREEVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPTK"
     gene            2938..3396
                     /gene="gpt"
                     /locus_tag="ACHY03_005584"
     CDS             2938..3396
                     /gene="gpt"
                     /locus_tag="ACHY03_005584"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFS3908640.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3488..4732
                     /gene="frsA"
                     /locus_tag="ACHY03_005585"
     CDS             3488..4732
                     /gene="frsA"
                     /locus_tag="ACHY03_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFS3908641.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            4790..5191
                     /gene="crl"
                     /locus_tag="ACHY03_005586"
     CDS             4790..5191
                     /gene="crl"
                     /locus_tag="ACHY03_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFS3908642.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5230..>6075)
                     /gene="phoE"
                     /locus_tag="ACHY03_005587"
     CDS             complement(5230..>6075)
                     /gene="phoE"
                     /locus_tag="ACHY03_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFS3908643.1"
                     /translation="LTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKYKDLGSFDYGR
                     NLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTLQY
                     QGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEA
                     WATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVL
                     SKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVAVG
                     MTYQF"
BASE COUNT         1475 a         1499 c         1542 g         1559 t
ORIGIN      
        1 acacccgccg tggggttgcc agtgctttgt taaaaccttg gattaagtct gaatccgaac
       61 ttacggtgga cgccagcata accgcaaaac ccttttttga acgttatggt tttcagacag
      121 ttaagcagca gcgcgttgaa tgccggggag cgtggtttac taatttttat atgcgatata
      181 aaccgcaaca ttaaatccag cttgcaatga aaataacgcc cgcctggtat gtgcgcgttt
      241 tcttattcca caaactgcaa ggaggtaaat catgacaaat cctttatcaa tgactctttg
      301 cagacctttc caggattaat tctttttttc ttgccctgga ttcgtctgcc atttcctgat
      361 ttttatattt atatactcta aataattcga gtcgcagttc atgcaacttg aagtatgacg
      421 agtataaatg gaatggtaca tgggcaaata tattcgtccc ttatccgatg cggtatttac
      481 catcgcatct gatgacctgt ggatcgagag tttagcgatc caacaattac acaccacggc
      541 aaatttaccc aacatgcagc gcgtagtggg gatgccagat ttacaccccg gacgcggcta
      601 cccgattggc gcagcgttct tctctgttgg tcgtttttac ccgacaagac gtcgcggtaa
      661 cggtgctgga aacagaaacg ggccgctact ctgacacgct gcgttcggcg ctgatttctc
      721 tggatggcga caacgcatgg gcgttaagcg aaagttggtg cggcactatt cagtggattt
      781 gtccgagtcc gtatcggcct catcatggac gcaaaaactg gtttctgggc attgggcgtt
      841 ttactgctga tgagcaggag caatccgacg cggtccgtta tgagacgctg cgttcgtcgg
      901 ggccgggcgg tcaacatgtc aataaaaccg actcggcggt acgcgccacg catctggcat
      961 ccggtattag tgtgaaggtt cagtcagagc gcagccagca tgctaacaaa cggctggcgc
     1021 gattgctgat tgcctggagg ctggagcagc agcaacagga aaatagcgcg gcgctgaaat
     1081 cacagcggcg aatgttccat caccagattg aacgtggcaa cccgcgacgg acatttacag
     1141 ggatggcttt tatcgaagga taatgaagga tgaaactgcc ggaaggtgat tgaacgccat
     1201 ccggcagcaa atcaaataat tacttcgtcg gaatttcttt cagcagttca gtcagcagtg
     1261 tccagtaatg acctacgctt tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga
     1321 tagttggccc gatagaaacc atgtccattt ccggatacgg ttttttgaac agaccacatt
     1381 ccaggcccgc gtggataatc tggatgttcg gcgtcttgtt gaacaggcgc tgataggttt
     1441 cacgtaccag atgcatcacc ggagaattag cgtccggctg ccagccagga tatgcgcctt
     1501 ttgcttcggt tttcgcgcca gccagtttac ccagcgaatc cagcatgctc accacgtagt
     1561 ctttaccgct gtcgatcaga gaacggatca ggcagtgaat ttctacgtta ttgtcagtca
     1621 tggtcaccac accgacgttc agggaggttt cgaccacacc ttttgccacg tcggaattgc
     1681 ggatcacacc gttcggggtg gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa
     1741 tcagggcagc tttatcgttc gctacagagt ccagcaacaa ggccagattt ttctctttct
     1801 ctgccagctc gtttttcagg atctcctgat aggtattcac cagagatttc agggcgtcga
     1861 ctttatcagc tgcgacagca atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg
     1921 tgccgccgtt gaaatcaata agacgcagat ccagttcttc tgcatgaccc gccaggaagc
     1981 gcaccagcag tttgttggca ttaccgaggc caacgtggat ttcaccgccg gagtgaccgc
     2041 ctttcagacc ttttaaggtt aacttgaagg tttcaaaacc agctggaacc tcttcacgat
     2101 ctaaatgcag gttggaggtg aagtcgatac cccccgcgca acccatgtag atttcacctt
     2161 cttcttcgga gtcggtgtta atcagaatat cagcctgcaa ccagttgctc tgtaagccaa
     2221 aagcaccgtc cataccggct tcttcggtca tggtcagcag cacttccagc ggaccgtgaa
     2281 ccacgttttc gtcagccaga accgccagtg cagaggccat accaatgccg ttatccgcac
     2341 ccagcgtggt gccgcgcgct ttaacccatt cgccatcaat ataaggctgg ataggatctt
     2401 tcgtgaagtc atgcacggtg tcgttatttt tctgcggcac catatcgagg tgggcctgca
     2461 agacgaccgg tttacgattt tccatacctg ctgtagcagg tttacgaatc aggatattac
     2521 ctacctgatc gcgttcgaca tggaaacctt tctcttttgc ccaaccaaca atgtattcag
     2581 cgagttgctc ttcatgatag gacgggtgag gaatagaaca gattttggca aaaatatccc
     2641 acagcggctg tggagataat tgagacagtt cagacacgtt aagtctcctt gtcgatcacc
     2701 cgcaaaacag tattgcaggt cacagggtta gcagaaaatg ttgtcaacac aagacaggct
     2761 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     2821 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     2881 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     2941 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     3001 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta
     3061 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     3121 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     3181 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     3241 gaaatgtatc caaaagcgca ctttgtcacc atctttgcaa aaccggctgg tcgtccgctg
     3301 gttgatgatt atgttgttga tatcccgcaa gatacctgga tcgaacagcc gtgggatatg
     3361 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     3421 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     3481 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     3541 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     3601 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     3661 gcgcggcatt gacccacgtg aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc
     3721 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggtg gcaactggat
     3781 ttatgagtgg gccacccagg cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc
     3841 gcaactcagt gggcgtcact ggctgcatgc ggctacgttg tacaacattg ccgcctatcc
     3901 tcatctgaaa ggagatgacc tggccgagca agcgcaggct ttgtcaaacc gcgcctatga
     3961 agaggccgct cagcgtctgc cgggcacgat gcggcagatg gagtttaccg tacccggcgg
     4021 tgcgcccatc accggctttt tgcatatgcc gaaaggcgat ggcccgttcc cgacggtatt
     4081 aatgtgtggt ggtctggatg cgatgcagac ggactattac agcctgtatg aacgttattt
     4141 tgcgccgcgc ggcattgcga tgctgactat tgatatgccg tcggtgggct tttcttcaaa
     4201 atggaagctc acccaggact ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa
     4261 cgtaccgtgg gtggatcaca ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt
     4321 tgccgtgcgt ctggcatacc ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc
     4381 ggtagttcat accctgttga gtgattttaa gtgccagcaa caggtgccgg aaatgtatct
     4441 tgacgttctg gccagtcgtt tggggatgca tgatgcttcc gatgaagcgt tgcgcgtgga
     4501 gctgaatcgc tattcattaa aagtgcaagg attgctggga cgtcgctgcc caacgccaat
     4561 gttatcaggc tactggaaga acgatccgtt cagcccggaa gaggactcac gcttaatcac
     4621 ctcatcatct gctgacggta aattattaga gatcccattt aacccggtgt atcggaattt
     4681 tgacaaaggt cttcaggaaa tcaccgactg gatcgaaaaa cgcttgtgtt aaaaatttgc
     4741 taaattttgc caatttggta aaacagttgc atcacaacag gagatagcaa tgacgttacc
     4801 gagtggacac ccgaagagca gattgatcaa aaaatttacc gcactaggcc cgtatattcg
     4861 tgaaggtaag tgcgaagata atcgattctt tttcgattgt ctggctgtat gcgtcaacgt
     4921 gaaaccggca ccggaagtgc gtgagttctg gggctggtgg atggagcttg aagcgcagga
     4981 atcccgtttt acctacagtt accagtttgg tctgttcgat aaagcaggcg actggaagag
     5041 tgttccggta aaagatactg aagtggttga acgactggag cacaccctgc gtgagtttca
     5101 cgagaagctg cgtgaactgc tgacgacgct gaatctgaag ctggaaccgg cggatgattt
     5161 tcgtgacgag ccggtgaagt taacggcgtg agtgaaatgt gccggatgca tcacatccgg
     5221 caataatcat taaaactgat aggtcatgcc aaccgcgaca atatcatcat tattaatatt
     5281 caatttgtta tcgctatcca gttggttgat tttataatca acaaacgctg acatattttt
     5341 gttgaaataa tatgtagccc cgacatcgat ataattgacc agatcttcat caccgatacc
     5401 ttcaatatct ttccctttcg ataagacata acccagcgat ggacgcagac caaagtcaaa
     5461 ctggtattga gcgaccgctt caaagttctg tgtcttattg gcaaagccgc cagttattgg
     5521 cgtcattttg cgtgtttcag aataaaaagt tgccagataa atattattgg catcgtattt
     5581 cagacctgta gcccatgctt ctgcacgctt gcctgtgcca cggctttgca ggttctgctc
     5641 gttggtgcga tctgagttgg tataggcccc actaatggcg aaatcgctgc cgccaaagtc
     5701 atatgtcaat gacgtgccga agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt
     5761 tttcccttga tattgcaggg ttaagttcag gccatcgata acgccgaaga agtcggtgtt
     5821 ccgatacgtc gccagaccgc tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga
     5881 gtcgccacca aattccggga acatatcggt ccaggcttcc acgtcataca acgcgcccag
     5941 gttacgacca taatcgaaag aacccaaatc tttatatttc aacccggcaa aagcgagacg
     6001 cgttttttgc tgtgcagtat cactctctgc tttattaccg gcaaactctg cttcccaacg
     6061 accataacca gtcag
//
LOCUS       DAZUCE010000741         5032 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.guided.297, whole genome shotgun sequence.
ACCESSION   DAZUCE010000741 DAZUCE010000000
VERSION     DAZUCE010000741.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.guided.297"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..327
                     /gene="frdD"
                     /locus_tag="ACHY03_005588"
     CDS             <1..327
                     /gene="frdD"
                     /locus_tag="ACHY03_005588"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFS3908644.1"
                     /translation="PVFWGLFGAGGMWSAIIAPVMILLVGILLPLGLFPGDALSYERV
                     LAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFYGLAAILTVVTL
                     IGVVTI"
     gene            390..1523
                     /locus_tag="ACHY03_005589"
     CDS             390..1523
                     /locus_tag="ACHY03_005589"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFS3908645.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELIAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1612..2145
                     /gene="blc"
                     /locus_tag="ACHY03_005590"
     CDS             1612..2145
                     /gene="blc"
                     /locus_tag="ACHY03_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFS3908646.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(2142..2459)
                     /gene="sugE"
                     /locus_tag="ACHY03_005591"
     CDS             complement(2142..2459)
                     /gene="sugE"
                     /locus_tag="ACHY03_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFS3908647.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2640..2786)
                     /gene="ecnB"
                     /locus_tag="ACHY03_005592"
     CDS             complement(2640..2786)
                     /gene="ecnB"
                     /locus_tag="ACHY03_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFS3908648.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2897..3022)
                     /gene="ecnA"
                     /locus_tag="ACHY03_005593"
     CDS             complement(2897..3022)
                     /gene="ecnA"
                     /locus_tag="ACHY03_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFS3908649.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(3074..3640)
                     /gene="efp"
                     /locus_tag="ACHY03_005594"
     CDS             complement(3074..3640)
                     /gene="efp"
                     /locus_tag="ACHY03_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFS3908650.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3682..4710
                     /gene="epmB"
                     /locus_tag="ACHY03_005595"
     CDS             3682..4710
                     /gene="epmB"
                     /locus_tag="ACHY03_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFS3908651.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRAGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1262 a         1276 c         1263 g         1231 t
ORIGIN      
        1 ccggtattct ggggcctctt cggggccggt ggtatgtgga gcgcaattat tgcgccggtg
       61 atgatcctgc tggtgggtat tctgctgccg ctggggctgt ttccgggtga tgcactgagc
      121 tacgagcgcg ttctggcgtt cgcgcagagc ttcattggtc gcgtattcct gttcctgatg
      181 atcgttctgc cgctgtggtg tggtttacac cgtatgcacc acgcgatgca cgatctgaaa
      241 atccacgtac ctgcgggcaa atgggttttc tacggtctgg ctgctatcct gacagttgtc
      301 acgctgattg gtgtcgttac aatctaacgc atcgccaatg taaacccggc ccgcctatgg
      361 cgggccgttt tgtatggaaa ccagacccta tgttcaaaac gacgctctgc gccttattaa
      421 ttaccgcctc ttgctccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca
      481 caattacccc gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc
      541 agggtaaacc ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca
      601 cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg
      661 gcgacgctat tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg
      721 aacttatcgc taaacagtgg aatgggatca cactattaca tctcgcaacc tacactgctg
      781 gcggcctgcc attgcaagtg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct
      841 atcaaaactg gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta
      901 tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc
      961 aaactcgtgt cttccagcca ctaaaactca accatacgtg gattaatgta ccgtccgcag
     1021 aagaaaagaa ttacgcctgg ggatatcgcg aaggcaaggc agttcatgtt tcgccagggg
     1081 cgttagatgc tgaaacttat ggtgtgaagt cgaccattga agatatggcc tgctgggtac
     1141 gaagcaatat gaatccccgt gatatcaacg acaaaacact tcagcaaggg atacaactgg
     1201 cacaatctcg ctactggcaa accggcgata tgtatcaggg tctgggctgg gaaatgctgg
     1261 actggccggt aaatcctgac atcatcgtta acggcagtga caataaaatt gcactggcag
     1321 cacgccccgt aaaagcgatt acgcctccaa ctcctgcagt acgcgcatca tgggtgcata
     1381 aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg
     1441 gtatcgtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctggc
     1501 agattctcaa cgctctgcag taaaatttca tcgggtccga attttcggac cttttctccg
     1561 cttttccttg ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg
     1621 ctccctctcg ttgccgcagc gacagctgca tttctggtcg ttgcctgcag ttctcctacg
     1681 cccccgcgtg gcgtgaccgt agtgaataat ttcgacgcta aacgctatct cggtacctgg
     1741 tatgagattg cccgttttga tcaccgcttt gaacgtggac tggaaaaagt caccgcaaca
     1801 tacagcctgc gtgatgacgg cggcctgaat gtcattaata aaggctataa ccctgacaga
     1861 ggaatgtggc agcagagtga agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg
     1921 ctgaaagtgt cattctttgg tcctttctat ggcggttata acgttattgc actcgatcgg
     1981 gaataccgcc atgcgctggt ttgcggcccg gaccgcgact acctgtggat actctcccgc
     2041 acgccaacca tttctgacga agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg
     2101 tttgatgtca gtaagtttat ttgggtacag cagcccggta gttagtgagt gctgagtttc
     2161 agaccaataa tccccaatac gattaacgcc agactcgcca gacgcatcgg gttagcggac
     2221 tcaccgagca gcacaatgcc ggtgatggcc gcgccgactg cgccaatacc cgtccacacg
     2281 gcataagccg tccctactgg taacgatttc atcgcccagg caagtagcgc catactgaca
     2341 atcatcgccg tcacggtaat aacactcggc gtcaaacgac taaagccgtg ggtatatttc
     2401 aggccaacgg cccataccac ttccagcaga ccagcaataa ctaagataat ccaggacata
     2461 tcaggctccg gagaattggg gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc
     2521 aaagctgatg gttgaacgga tattttgcca ggtggcagag gaatttcaac tattaccttt
     2581 gtgactatcg caaaaaaaca aacggcacga cacagctgtc gtaccgttat tgcttattgc
     2641 tattgctgcg ctttcgttgc tgcgccagaa atcgcgttac cgccatcaga aatgtcttca
     2701 ccaacgccac gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag aacagaaaag
     2761 atcgctgcaa ttgtcttctt caccataacg tcttcctttt aggtaatgtt atttatgttt
     2821 gcctatagca aattaataat agacaacatc gccaaaaatg acggaaatct gatgattttt
     2881 cggaagagaa gaaaaattag ctggcagcgc gggagatgga gttgccgaga tgtttgatgt
     2941 cttcgccgaa accgcgagcg gtgttacagc ccgtgagcag cgtgctggca agcaaaacaa
     3001 gaacgataag gcgtttcatc atctctgacc tgaataagtg atggtgcagc ctgcaggccg
     3061 caccacaacc gcattacttc acgcgagaga cgtattcacc agagcgggta tccactttga
     3121 tgacttcgcc gatttgtacg aacagcggaa ctttaaccac agcgccagta gacagggtag
     3181 ccggtttgcc gccagtacct gcggtatcac ctttcaggcc cggatcggta tcaacgattt
     3241 ccagttcaac gaagttcggc ggagtaacgg agatcggctg accattccac agagttacga
     3301 tacactctgc ctgatccagc agccatttag cgttgtcgcc aattgctttt gcgtcagcag
     3361 acagctgctc gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg ttgtacaggt
     3421 aagtcaggtt catatcgaca acatcagcgc cttcagcgga atcagtagat ttgaaggttt
     3481 tttctacgcg agtaccggtc agcagacgac gcagtttaac gcgagcaaat gcctggcctt
     3541 tacccggttt tacgaattca ctcgcttcaa ccgcgtaagg ttcgccgtct aacatgattt
     3601 taagaccagc acgaaaatcg ttgctatagt acgttgccat aaggccctct gaaatttgtt
     3661 aattggtagc taagccacaa aatggcgcat attgtaaccc taaatacccc atccagagaa
     3721 gattggttaa cgcaacttgc cgatgttgtg accgatcctg atgaacttct gcgtcttttg
     3781 aatatagacg cggacgaaaa actgttggcc ggacgcaacg ccaaaaagct gtttgccctg
     3841 cgtgtgcccc gctcatttat cgatcgcatg gagaaaggca atccggacga tccgcttttg
     3901 cgtcaggtac ttacctcgca agatgagttt gccatcgcgc ccggattctc caccgacccg
     3961 ctggaagaac agcacagcgt agtgcctggt ttgttgcata aataccacaa ccgggcgctt
     4021 ttgctggtca aaggcggctg cgcggtaaat tgccgctatt gcttccgccg ccacttcccc
     4081 tatgccgaaa atcagggcaa caagcgtaac tggcaaactg cacttgagta tgttgctgcg
     4141 catccggaac tggacgagat gattttttcc ggcggcgatc cgctgatggc gaaagatcac
     4201 gagctggact ggttgctcac acaactggaa gccatcccgc atatcaaacg tctgcggatt
     4261 cacagtcgtc tgccgattgt gatcccggca cgcatcacag atgcgctggt tgaacgcttt
     4321 tcacattcta cgctgcaaat cttgctggtg aatcacatca accatgccaa tgagatagat
     4381 gaaacattcc gtcaggcgat ggctaagttg cgccgggcgg gcgttacttt gctgaaccag
     4441 agcgttctgt tacgtggtgt gaacgataac gcacaaacgc tggcaaacct aagtaatgcg
     4501 ttgttcgatg ccggcgtgat gccgtattac ctgcatgtgc tcgataaagt acagggcgcg
     4561 gcgcatttta tggtgagtga tgacgaagca cggcagatta tgcgtgagtt gctgacactg
     4621 gtgtcgggat atctggtgcc gaaactggcg cgagaaattg gcggcgaacc cagcaaaacg
     4681 ccgctggatc tccagctacg ccagcagtaa ctgattaaac agggaagtca aacattaaaa
     4741 ttgcctgatg cgctacgctt atcaggccta catcgtgcat tgaaattgaa tggatttgca
     4801 atgaactgta ggccggataa ggcgtttacg ccgcatccgg caagagcaac aaacgcaagt
     4861 acagcaacct gtcattaaac tgggaaattt aatctttgcc cgttgcataa cctttaatta
     4921 ttcatatgca ttgctgttgt tgctctttat ataactaaca tcaataatta acctttcttt
     4981 tctgtttata tagagtgagt acggctattt atgttatttt aacaactatt ac
//
LOCUS       DAZUCE010000742         2027 bp    DNA     linear   BCT 26-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 1802M77273
            SAMN43251508-rid23080193.guided.404, whole genome shotgun sequence.
ACCESSION   DAZUCE010000742 DAZUCE010000000
VERSION     DAZUCE010000742.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43251508
            Sequence Read Archive: SRR30292943
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002349794.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002349794.1
            Genome Coverage       :: 100x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:57:27
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,598
            CDSs (total)                      :: 5,513
            Genes (coding)                    :: 5,360
            CDSs (with protein)               :: 5,360
            Genes (RNA)                       :: 85
            rRNAs                             :: 3, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 60 of 153
            Pseudo Genes (incomplete)         :: 100 of 153
            Pseudo Genes (internal stop)      :: 34 of 153
            Pseudo Genes (multiple problems)  :: 31 of 153
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43251508-rid23080193.guided.404"
                     /serotype="O166:H28"
                     /isolate="1802M77273"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2018-02"
                     /collected_by="SSI"
                     /note="subtype: stx1c"
     gene            <1..163
                     /locus_tag="ACHY03_005596"
     CDS             <1..163
                     /locus_tag="ACHY03_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFS3908652.1"
                     /translation="DTCIGKRLHKAEGIVEGMLMMLGVRLEMDRYVERELPGGRTSVF
                     YQRKNSLRS"
     gene            671..1618
                     /gene="stxA1c"
                     /locus_tag="ACHY03_005597"
     CDS             671..1618
                     /gene="stxA1c"
                     /locus_tag="ACHY03_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1c subunit A"
                     /protein_id="HFS3908653.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGIPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSVNAILGSVALILNCHHHASRVARIVP
                     NEFPSMCPVDGRVRGITHNKILWDSSTLGAILIRRAISS"
     gene            1629..1898
                     /gene="stxB1c"
                     /locus_tag="ACHY03_005598"
     CDS             1629..1898
                     /gene="stxB1c"
                     /locus_tag="ACHY03_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1c subunit B"
                     /protein_id="HFS3908654.1"
                     /translation="MKKILLIAASLSFFSASVLAAPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          549 a          344 c          491 g          643 t
ORIGIN      
        1 tgacacctgt ataggtaaac gccttcacaa agcggagggg attgttgaag gcatgctgat
       61 gatgctggga gtgaggcttg agatggatcg gtatgttgag cgtgaattgc cgggagggag
      121 aacctctgta ttttatcagc gaaaaaatag tttacgatcg taaaaatctg catatcatga
      181 taagagtggt tacattgcta cgctgcttaa cccgccgatg cgcgggtttt tttgtaccca
      241 gaatcctgtg agctatacgg aaagtacaca gaaaggaagg tgcgaccgta attaataaca
      301 aaatcttaaa aatcgcatat agcactatta gttttctaaa tattgtatat tttaagtatt
      361 gcaggatgac cctgtaacga agtttgcgta acagcatttt gctctacgag tttgccagcc
      421 tcccccagtg gctggctttt tttatgtccg taacatcctg tgtatcaata aatgttgttg
      481 tctacgtacg tcaagtagtc gcatgagatc tgaccagata tgttaaggtt gcagctctct
      541 ttgaatatga ttatcatttt cattacgtta ttgttacgtt tatccggtgc gccgtaaaac
      601 gccgtccttc agggcgtgga ggatgtcaag aatatagtta tcgtatggcg ctcaaggagt
      661 attgtgtaat atgaaaataa taatttttag agtgctaact tttttctttg ttatcttttc
      721 tgttaatgtg gttgcgaagg aatttacctt agatttctcg acagcaaaga cgtatgtaga
      781 ttcgctgaat gtcattcgct ctgcaatagg tattccatta cagactattt catcaggagg
      841 tacgtcttta ctgatgattg atagtggcac aggggataat ttgtttgcag ttgatgtcag
      901 agggatagat ccagaggaag ggcggtttaa taatctacgg cttattgttg aacgaaataa
      961 tttatatgtg acaggatttg ttaacaggac aaataatgtt ttttatcgct ttgctgattt
     1021 ttcacatgtt acctttcctg gtacaactgc ggttacattg tctggtgaca gtagctatac
     1081 cacgttacag cgtgttgcgg ggatcagtcg tacggggatg cagataaatc gccattcgtt
     1141 gactacttct tatctggatt taatgtcgca tagcggaacc tcactgacgc agtctgtggc
     1201 aagagcgatg ttacggtttg ttactgtgac agctgaagct ttacgttttc ggcaaattca
     1261 gaggggattt cgtacaacac ttgatgatct cagtgggcgt tcttatgtaa tgactgctga
     1321 agatgttgat cttacgttga actggggaag gttgagtagt gtcctgcctg actatcatgg
     1381 acaagactct gttcgtgttg gaagaatttc ttttggaagt gttaatgcaa ttctgggtag
     1441 cgtggcatta atactgaatt gtcatcatca tgcatcgcga gttgccagaa ttgtacctaa
     1501 tgagtttcct tctatgtgcc cggtagatgg aagagtgcgt gggattacgc acaataaaat
     1561 attgtgggac tcatccactc tgggggcaat tttgatacgc agggctatta gcagttgagg
     1621 ggggtaaaat gaaaaaaata ttattaatag ctgcatcact ttcatttttt tcagcaagtg
     1681 tgctggctgc gccagattgt gtaactggga aggtggagta tacaaaatat aatgatgacg
     1741 atacctttac agttaaagtg ggagataaag aattatttac taacagatgg aatcttcagt
     1801 ctcttcttct cagtgcacaa attacgggga tgacggtaac cattaaaact aatgcctgtc
     1861 ataatggagg gggattcagc gaggttattt tccgttgact cagaatagcc cggtgaagag
     1921 aacaggcgga gatttatcaa tgttaaatac atctcaattc agtcagttga ttgttgcctg
     1981 tctgataata gatgtgctac aaaatttctg catggtgaat ccccctg
//
